{ "genome": { "genus": "Mycoplasmopsis", "species": "fermentans", "strain": "JER", "taxon": "Mycoplasmopsis fermentans JER", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 977524, "gc": 0.2694606674216361, "n_ratio": 1.0229927858548741e-06, "n50": 977524, "n90": 977524, "coding_ratio": 0.6519897741536517 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 100, "stop": 270, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAGAATAAAAATATAAAAGATAGTATCACAATGAAAGCAAATACATTAACATTTTTAAAATCTTTAAGTTCAGATGTTAATGACCAAATGTTGTTCAAAAATTTCATTTCAAACTTCACAATTGTAAGCATAGATCAGAACAATCATGTCAAAATTCAAACATGA", "aa": "MAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVSIDQNNHVKIQT", "aa_hexdigest": "c745473b2fcc072dd05335d200d90a3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6392.251699999999, "isoelectric_point": 8.12064723968506 }, "id": "IGIGPGHMMC_46", "locus": "GCA000148625_00001" }, { "type": "cds", "sequence": "contig_1", "start": 304, "stop": 1044, "strand": "+", "frame": 1, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A168RAH0", "UniRef:UniRef90_C4XFM3" ], "nt": "ATGCTAGATGGTAATATTTTAAAAGCTTTAGAAGAAACTTTTGAAAGAGAATGCACATATGAATTTGTTCATATTCCTAAACCTATTGTTAAATTAACAAAAGCCTCAAGCAACAAAACAATTTCTTCTAAAACAATTGAAGAAGAAGTGAAATTAGGCGTTGATTTAAATAAAGAATTAACATTTGACAACTATGTTGAAAGTGATTTTAATAAAGATGCAATCAAATTAGCTAACTATTTAGTTAAAGGTGGAAAAGAATATAATCCTATTTTTATTTATGCTAAAAGTGGACTAGGAAAAACACACTTATTACATGCAATTGGTAACAAATTACAAGAGCAAGGCAAAGTTGTTAAATACATTAATCCAAGCATTTATGCTAGAGATATATCATTCTATTTGCAAGAAAATGATCAAGCAAAATTAAAGAAAATAAAAGATGAATTTGATAATGCTGATTACTTAATGTTTGATGATTTTCAAAGTTATGGAGTTGGAAATAAAAAAGCAACAATTCAAACAATTTATAATATTTTAGACTATCGTTTGAACACAAATAAAACAACCATATTTTGTTCGGATAAGCCTATAAATAACTTGAATGCGATGTTTGATGAAAGATTAATTTCTCGTCTTTATATGGGTTTACAACTTGAAATTAAAATTCCACAACAAAGTGATTTATTAAGAGTTTTAGATTACATGCTAGATATTAATGATATGAATCCAGAGGCTTGA", "aa": "MLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLMFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEA", "aa_hexdigest": "e56ec54b55463e5b8c9c77adb01a33f5", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "both", "pseudogene": { "start": 97, "stop": 1347, "observations": { "mutation": [ 268, 1042 ], "stop": [ 268, 1042 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM3", "query_cov": 1.0, "subject_cov": 0.5222929936305732, "identity": 0.996, "score": 485.0, "evalue": 1.04e-169, "gene_start": 1, "gene_end": 246, "reference_start": 70, "reference_end": 315, "reference_sequence": "MMAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVGIDQNNHVKIQTWLQKPSLEILIKMLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLIFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEAWEKEAKNFVTRNYSTSIRTLIGAITRLKFYKMEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK" }, "paralog": false, "identity": 0.9879807692307693, "score": 804.0, "evalue": 6.7e-298, "description": "Internal stop codon at 268, 1042. Nonsense mutation around 268, 1042.", "psc": { "uniref90_id": "UniRef90_C4XFM3", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A168RAH0", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM3", "UniRef:UniRef50_A0A168RAH0", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A168RAH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RAH0" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "IGIGPGHMMC_47", "locus": "GCA000148625_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1135, "stop": 1512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGGAAATGGCCCGGACTAATTCTAAATATACTGTTGCTGTTGTTAATTCAATATTAAAAGACATGCAACAAACAAAAGAAAATATTACACCAGATTCAATAATAGAATATGTAGCAAAATATTACAAAATTCCTAAAAAAGAAATCTTAGGCAAAAGCAGACAAAAAGATGTCGTTCTAGCAAGACACATAGCTATTTATGTCATAAGAACACAACTGGAAGTTCCATTAGAACAAATTGGTCATATATTTGGTAATAGAGATCACTCAACTATTATTAATGCAATTAGAAAAATAGAAACTGAAAGTGATATGCCAGATAAAACAATTAAAAGAACCATATCAATGATTAGTGATGATATCTACAAATTAAAATAG", "aa": "MEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK", "aa_hexdigest": "367f31c02b7669217b6976ba446b42c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 69, "aa_cov": 0.552, "hmm_cov": 1.0, "evalue": 8.134020177625593e-28, "score": 92.33539581298828, "start": 31, "stop": 99 } ], "seq_stats": { "molecular_weight": 14478.732099999987, "isoelectric_point": 9.396283149719238 }, "id": "IGIGPGHMMC_48", "locus": "GCA000148625_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1643, "stop": 2461, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_C4XFM2", "UniRef:UniRef90_C4XFM2" ], "nt": "ATGAAATTACACATTAAAAAACAAATTTTAGATGAAACATTGGAAATTGTTTCAAAGTATACAGATCCTATTAATTCATTGTATGGGTTCAGATGTATATTAATTAAAGTAGATAATGAAAAAGTAACATTTTCAGCATCTAATGGAGTTATAAGTATAGTTAAATCACTAGATGTTGAAGATGTGGATATTAAAGTAGAAGAAACAGGAACATTTTTAATTCAAGCAAATATATTTAAAGCTATTATTAAAAAATTAAGTGAAACAATTACTTTAAGTCAAGAATATTCAACAACATTAGAAATATCACAAGGCAATTCAAATTATTCATTAACTACAAACCCAATCAATTCATTTCCACAAATAGAAGAAAATATTAATTTAAAAAGAATTGAAATCGATACAAATGAATTTAAAAAAGCTATTAGATGCACAGCTTTTGCTGTTAGTCAAGACGCTTCATTAGTATATAAATGTATTAATTTTAAATTTAAAAATAATAAATTAAATCTAACAGCTACAGATGCAGCAAGACTTGCATATTACACAATGGATCTTAAAAGTTCGCAAGTAGATAATGAAGAATTTTCAGTAAACAATAAAGATGTTAAAGATTTAATACCTACAGATGCATCTAAAAAAGTAACATTATTCTTTAATAGAATAAAAATAGGAATTGAATATAAAAATACAGTTATCACTTCAAGAATAGTTGATTTGCCTTACCCAGATGTTGAAAGTTTATTTGCTTCAATAGATATTGAAAATAAAATATTTATTAAAAAAGAAGAAATAAATGATTTAATTAACAAAGTTTGA", "aa": "MKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKRIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPTDASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKV", "aa_hexdigest": "e9cbcf16a5ba0a6042b74d523205a9f4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaN" ], "truncated": "3-prime", "pseudogene": { "start": 1640, "stop": 2749, "observations": { "mutation": [ 2459 ], "stop": [ 2459 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM2", "query_cov": 1.0, "subject_cov": 0.7371273712737128, "identity": 0.993, "score": 513.0, "evalue": 6.49e-182, "gene_start": 1, "gene_end": 272, "reference_start": 2, "reference_end": 273, "reference_sequence": "MMKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKKIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPADASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKVWLGTSDKQNRLEFTINKNEITVLNRLNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR" }, "paralog": false, "identity": 0.986449864498645, "score": 704.0, "evalue": 1.62e-259, "description": "Internal stop codon at 2459. Nonsense mutation around 2459.", "psc": { "uniref90_id": "UniRef90_C4XFM2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_C4XFM2", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM2", "EC:2.7.7.7", "UniRef:UniRef50_C4XFM2", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM2" ], "product": "DNA polymerase III subunit beta" } }, "id": "IGIGPGHMMC_49", "locus": "GCA000148625_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2516, "stop": 2749, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTTTTAAATAGATTGAATGAAATCGGAACATCTATTGCAAAAACAGAAAATTTTAAATTTGAAGGAAAAGCTTTTGAATTTGATATTAACTATCATTTCTTAAAAGATGCTTTAAGTGTGTTTGAAGGTGACGTTTTAATTCTATTTGATAAAAGTGTACAAAAAATTTTAGTAGTTTCAGATTCAAATAAAGAATCTAAACAACTAGTAACTCCTATGAGAAGATAA", "aa": "MTVLNRLNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR", "aa_hexdigest": "a0261825713e0b51492888d3406e7557", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8855.112299999997, "isoelectric_point": 6.561231803894043 }, "id": "IGIGPGHMMC_50", "locus": "GCA000148625_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2751, "stop": 2972, "strand": "+", "frame": 3, "gene": null, "product": "RNA-binding S4 domain-containing protein", "db_xrefs": [ "KEGG:K14761", "RefSeq:WP_013354278.1", "SO:0001217", "UniParc:UPI0001E32ECF", "UniRef:UniRef100_UPI0001E32ECF", "UniRef:UniRef50_A0A3P8LAR7", "UniRef:UniRef90_C4XFM1" ], "nt": "ATGAATAATGAAATTTATTTAAAAGATGATGTAATTACACTAGGTCAATTATTAAAGAAAATAGGACTTATTGACACAGGCGGATCAGCTAAAGGTTATTTACTTTCTAATAAAATTTTAATAAATAATAGAACACCAAATGGACGTAGTGCAAAAATTAAAATTGGTGACATTGTGTGGATAAATAATAAAGTTTTTATGATTAAAAAACTTGAAAAATAA", "aa": "MNNEIYLKDDVITLGQLLKKIGLIDTGGSAKGYLLSNKILINNRTPNGRSAKIKIGDIVWINNKVFMIKKLEK", "aa_hexdigest": "7b8119034ea6eb927f1798f34e65f052", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001E32ECF", "ncbi_nrp_id": "WP_013354278.1", "uniref100_id": "UniRef100_UPI0001E32ECF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32ECF", "RefSeq:WP_013354278.1", "UniRef:UniRef100_UPI0001E32ECF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32ECF", "product": "RNA-binding S4 domain-containing protein", "uniref90_id": "UniRef90_C4XFM1" }, "psc": { "uniref90_id": "UniRef90_C4XFM1", "uniref50_id": "UniRef50_A0A3P8LAR7", "kegg_orthology_id": "K14761" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LAR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LAR7" ], "product": "S4-like RNA-binding protein" }, "genes": [], "id": "IGIGPGHMMC_51", "locus": "GCA000148625_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3128, "stop": 6160, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0", "UniRef:UniRef90_C4XFM0" ], "nt": "ATGAAAAAAATAAAGAAAATAAGTTTGGCTATTGTTCCTGCAATTGCAACAGTAACAACTTTACCGTTGACTACTATTTCATGTCATAAAGATCCTAAGGAAACTCCAAAATCTCTTACTGAAACAGAACTAAATAGTTTATTAGAAAAAATCAAAGTAACTTTATTAGATAAAAATTTAACTGCTGATGAAGTTATAGCTAAAGGCAAAGACATTTTTAAATTTACAGATGTAGATACTAAAGTTAATTTTAAAATTGTATCAATTAAAAAAGGTGCAAATAAAGGTGAAGTTGAATTAACACTTAAAGTTGTTGATAAACAAAACAAAGACAAAATGTCTAAAGAAAAAACTTTTACATTTAAATTAGAGGCTAAAGAAAACTTAGATGATCTTTTAGAAAAAGTACAAGTTACTTTAAAAAACAATTTATATAGTAGCGAAGTGGTTTCACAAGGAAAAGATGCCTTCGAATTTGCTAGCATTGATGCTAGAGTAAATTGTGAAGTAGTATCATTAGAAGAAAATACCGATAAAACAGGAGTTATTTTAAAAATTAAAGTTGTTGATAAAAAAGATTCAAATAAATTCTCTAAAGAAAAAACATTTGAAATAAAAACTTTATTAACACCTGATATGGTATTGAATCAAATTAAAGCTGAATATCCAGATCACTTAAATGTGTTTGTTACAGATGCTAATATAAATAAAATTGTTTACTCTAATTTTGACCAAAACACCTTTGAAATAGTTGAATCTAAACTTTTGAGTAAATTTTATTCAACATTAAAATTTGAATTTAAAATTAAAAACAAAATTAGAAATGAAGTTTCTACAAATAAAAGAACTATACAAATTGATGGTTTTAAATTAACAGACAAATATTTAAATTATCAACTCGGAAATATTGAAATTTATTCAGATGGATCTAAAACTTTAAAAGAAACAAAAACTGAACTTTTAAATCCAAATACAACAACATACAAAACAATATTTATTAATAACTTACCATCAACAGTAAAAATTGTTTTTGAATCTGTTGAAGTATATAAAGAAAATGCTGATCAGTTAGAAATTAAATTTCATCTTACAAATATTCACAATGAAAACAATAAATCTAATACTAGAACTGAATACATATATCACAAAAGTGATCATTATGATGCTTTAGTTCAAGAATTTGAAAAAGCTAAATTATCATATGAAGGCGGTACTAACAAAGTAAATGCTTTTGATATTTTTGAAAGCAAAATTAAAATAGAAGGACTTAAAACACCATCAGATTTTTCAGATTTTGTTTTTAAATTCACAAATTCTAGCCCTAGCTCTACTGAATACAAAAGTGGTAAAAGAAAAATTGATTTTAGTTTTGTTTATAAAGGAAACAAATTCTTCAGAAGTGAACAAGAACTTCAAGTAAGACCAACAACGAATGAAATAATTACATCATTAAAAAAACTTAATAAAATTAAACTTGTTGAAACAACTGAAGCTTTAAATAAATTAGAAAGCTTAGTTGATGGTACAACTTTAGATGCATTTAACAATTCTATTTCAATAGATGGGGTTGAATTATTTAGTTACAATTTTACTGCATATCTATTTTCTGAAGATAATGATACTTCAGCTAAAAAAGTATTACTAAATAAAGATAATGAAAACTATTATTTAAGTTTCTACTTAGGTGGAAAAGTATATGGCAAATATGTAAGACACTATGAAGAAAGTAGCACTACTATAGCTAAAATCCAAACACCTGCTGAATTGACAGCAATGGTAAATTCAAAAATTAATGAATTTGATTATAAAAACAAAACAACAACAAAAGCTAAAAATGCCATTCAAGAAAACATTGTTAGCCCAGATTTTGGCTCAGGTTACAGCGTTAAAATTTCAGACTTCACAACTTTAAATGACAAAATTACATTTAATGTTCAATTAAGTAATTCTCAACATCCAGATGTTTTCTCTAATAAAGTTCCAATTACAATTTCAGGTTTCTACCGTAGCGAATTAGCAGAAGAAATCACTAACATGGAAGTTACAATTGCTGATTCATTTAAAGCTAAAGAAGCTAAAGAATTCACAGATGTAAACAACATTACAATCACAGACAAAAGAACAAGTACTGCTTATGATTTTGCAACTAAAGGTGTTTCTCTTGAAACAACTATTGTTGAAGTTAAAAATTTAGAAGGTATTATTAAATTCAACTTAAAATTCACAAAAGAAACTGAAGTTATTAATAAAGAATACATCATTAATGATTTCAAGAAAGAAACTTTAACAGCTGAAAAAATTGTTAAGCTATTAAATATCCAATTAATTAAAGAAAATGTTCAATTAGACAAAAAATACTTCTTGTCTTCATATATTACAAAAAATGAAGTATCTATTACAAACTTAGATGATCTCAAAAAAATAAATTCAACAGTTGATGTTGTTATTAATAAAATAACACCTAATGTTGCTGAAGGTAAATTAGACATTGAATTAAACATTTCGTTTGATTCAAATTCAAATACTAAAACAATTTCAGTCAGTGGCTTTAACAAAAACGTTAAATCTTCAACTGAAAATATGATATTTAAAGACAAAATGAAGTTAATTGTTTACTTGGCAAAAAATAATATTTTGTTCAAACAAGTAGATCAAAACACAATTAATGAAATGAATGTTGCAGGTCCATTTGGCATGACAACATCTATTAAACAAGGCTTAACAAAATATGGTTTTAACCAAATGACAATGGAAAACAACTTCAATTTTGAATATGTATTTAACTTTGTTTTAGCTGATACTGAAACAGTTCAAGATGAAAAATTTGATACTTTAATTAGACATCATATTGTTGGTAATAAATATAATAAAGATCAACCGATGTCTCGCCACATAAAAGTTTTTAGAATAAATGGTAATACACAATTAGCTGCATCATTTAGAATTGTTGCTAGAGATCATGTTAAATTTGATTTAACTAAATTTGAATATAAAACATCAGTTGACGATGATGAATATAAAGTATTTAAATGA", "aa": "MKKIKKISLAIVPAIATVTTLPLTTISCHKDPKETPKSLTETELNSLLEKIKVTLLDKNLTADEVIAKGKDIFKFTDVDTKVNFKIVSIKKGANKGEVELTLKVVDKQNKDKMSKEKTFTFKLEAKENLDDLLEKVQVTLKNNLYSSEVVSQGKDAFEFASIDARVNCEVVSLEENTDKTGVILKIKVVDKKDSNKFSKEKTFEIKTLLTPDMVLNQIKAEYPDHLNVFVTDANINKIVYSNFDQNTFEIVESKLLSKFYSTLKFEFKIKNKIRNEVSTNKRTIQIDGFKLTDKYLNYQLGNIEIYSDGSKTLKETKTELLNPNTTTYKTIFINNLPSTVKIVFESVEVYKENADQLEIKFHLTNIHNENNKSNTRTEYIYHKSDHYDALVQEFEKAKLSYEGGTNKVNAFDIFESKIKIEGLKTPSDFSDFVFKFTNSSPSSTEYKSGKRKIDFSFVYKGNKFFRSEQELQVRPTTNEIITSLKKLNKIKLVETTEALNKLESLVDGTTLDAFNNSISIDGVELFSYNFTAYLFSEDNDTSAKKVLLNKDNENYYLSFYLGGKVYGKYVRHYEESSTTIAKIQTPAELTAMVNSKINEFDYKNKTTTKAKNAIQENIVSPDFGSGYSVKISDFTTLNDKITFNVQLSNSQHPDVFSNKVPITISGFYRSELAEEITNMEVTIADSFKAKEAKEFTDVNNITITDKRTSTAYDFATKGVSLETTIVEVKNLEGIIKFNLKFTKETEVINKEYIINDFKKETLTAEKIVKLLNIQLIKENVQLDKKYFLSSYITKNEVSITNLDDLKKINSTVDVVINKITPNVAEGKLDIELNISFDSNSNTKTISVSGFNKNVKSSTENMIFKDKMKLIVYLAKNNILFKQVDQNTINEMNVAGPFGMTTSIKQGLTKYGFNQMTMENNFNFEYVFNFVLADTETVQDEKFDTLIRHHIVGNKYNKDQPMSRHIKVFRINGNTQLAASFRIVARDHVKFDLTKFEYKTSVDDDEYKVFK", "aa_hexdigest": "b65b535c482ee43764eebd8713bc81d8", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XFM0", "query_cov": 1.0, "subject_cov": 0.9940944881889764, "identity": 0.9990000000000001, "score": 1905.0, "evalue": 0.0, "valid": true, "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XFM0" }, "pscc": { "uniref50_id": "UniRef50_C4XFM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0" ], "product": "Lipoprotein-associated type-17 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_52", "locus": "GCA000148625_00007" }, { "type": "cds", "sequence": "contig_1", "start": 7049, "stop": 7261, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354280.1", "SO:0001217", "UniParc:UPI0001E32ED1", "UniRef:UniRef100_C4XFL9", "UniRef:UniRef50_C4XFL9", "UniRef:UniRef90_C4XFL9" ], "nt": "ATGCTATGTCAAACAAGTTATACACAAAGCGTCCAATATTATGAAAAAGCAAATTTTGTTTATATTGTTTTTTCTCTTAAATATGTTTATAGTGATGGCGAAAAAGTATCATTAAAAAATATGACTATTAAAAAATACAAAAAAATCACAAATTATAAAAAAGAACAATTCATTAAAGATAACTTAGAATTGGCAGTTTCGGAGAAATTTTAA", "aa": "MLCQTSYTQSVQYYEKANFVYIVFSLKYVYSDGEKVSLKNMTIKKYKKITNYKKEQFIKDNLELAVSEKF", "aa_hexdigest": "2624e3ad5ee27e47fe5d8d3229bb11f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32ED1", "ncbi_nrp_id": "WP_013354280.1", "uniref100_id": "UniRef100_C4XFL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32ED1", "RefSeq:WP_013354280.1", "UniRef:UniRef100_C4XFL9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFL9", "uniref90_id": "UniRef90_C4XFL9" }, "psc": { "uniref90_id": "UniRef90_C4XFL9", "uniref50_id": "UniRef50_C4XFL9" }, "pscc": { "uniref50_id": "UniRef50_C4XFL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8399.661499999998, "isoelectric_point": 9.301772499084471 }, "id": "IGIGPGHMMC_53", "locus": "GCA000148625_00008" }, { "type": "cds", "sequence": "contig_1", "start": 7485, "stop": 7742, "strand": "-", "frame": 3, "gene": null, "product": "CMP/dCMP-type deaminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7", "UniRef:UniRef90_C4XFL7" ], "nt": "ATGAATGCAATTCTAAATACAAACAATAATCTTCAAGGTTGCACAATCTTCACTTCTTTATATCCATGCTCAAGTTGTGCTAAGACCATAGCTCAATCAGGCATTAGTGAAGTAGTTTATGAAGATGACAAATATCATGACACTGAAGACGCAGAAATAGCAAGGCACATTTTAAATGCAAGCAATATTAAAACAAGAAAAATTGAAATAGATACAAATGTTGAAGTTCAAGTGGGTAAAAACAAATACAAAAATTAG", "aa": "MNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN", "aa_hexdigest": "804a9da7e38b9b189bcd6b75ea1f94ac", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 7485, "stop": 7976, "observations": { "mutation": [ 7802, 7955 ], "stop": [ 7802, 7955 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL7", "query_cov": 1.0, "subject_cov": 0.5214723926380368, "identity": 1.0, "score": 167.0, "evalue": 2e-51, "gene_start": 1, "gene_end": 85, "reference_start": 79, "reference_end": 163, "reference_sequence": "MKNKSLYWDGYFMALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFPWDREGESPKETKYAYVVHAEMNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN" }, "paralog": false, "identity": 0.9877300613496932, "score": 324.0, "evalue": 6.05e-116, "description": "Internal stop codon at 7802, 7955. Nonsense mutation around 7802, 7955.", "psc": { "uniref90_id": "UniRef90_C4XFL7", "product": "CMP/dCMP-type deaminase domain-containing protein", "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL7", "UniRef:UniRef50_C4XFL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7" ], "product": "CMP/dCMP-type deaminase domain-containing protein" } }, "id": "IGIGPGHMMC_54", "locus": "GCA000148625_00009" }, { "type": "cds", "sequence": "contig_1", "start": 7800, "stop": 7940, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00383.29" ], "nt": "ATGGCTTTAGCTAAAGTATCTGCATTAAGATCTAAAGATCCTTCAACAAAGGTCGGTGCTTGCGTAGTTAATGAAAACAAAAGAATTATTGCTCTAGGTTACAATGGAATGCCAAAAGGCATAGATGACGAATTCCCTTGA", "aa": "MALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFP", "aa_hexdigest": "d7330ea7e3051064131807cc2b734702", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00383.29", "name": "dCMP_cyt_deam_1", "length": 42, "aa_cov": 0.8913043478260869, "hmm_cov": 1.0, "evalue": 1.715598336722577e-08, "score": 30.32900047302246, "start": 1, "stop": 41 } ], "seq_stats": { "molecular_weight": 4905.6738000000005, "isoelectric_point": 8.978270149230958 }, "id": "IGIGPGHMMC_55", "locus": "GCA000148625_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8192, "stop": 8572, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6", "UniRef:UniRef90_C4XFL6" ], "nt": "ATGGCTAAAGATCCTTCAAAAATGGATGAATATCTTAAATACATTGAAGACGGGGGGTTCCACAAATCAATTACCCTTATACCTAAATACAATGATTATTTAGTTAAAGGTGGAAGACTGCATCCTGCTGCCGCAGCCATAGCAAAATTATTTAAAATTGTTCCTTTAATTGCTGTTGATGGTGGTCAACTTAAAAAAGAAGGCGTCGGTCATGTTTTTTCAAAAACCACTATTAAAAATATAGTATCTAAAGCTAAAAACTTCAAAATCAATGACAAGAAAAATGTACTTCTTACTGCTTATATACACACAGGTGCTTCAGAAGAAGAACAAAAAACTTTTATTGAAGAATTCAAAAAACAATACCACCAAGACCCTTGA", "aa": "MAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDP", "aa_hexdigest": "6913da68583222c0c0779a18b0f73455", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "both", "pseudogene": { "start": 8060, "stop": 8872, "observations": { "mutation": [ 8095, 8185, 8194, 8587 ], "stop": [ 8095, 8185, 8194, 8587 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL6", "query_cov": 1.0, "subject_cov": 0.42, "identity": 1.0, "score": 250.0, "evalue": 8.65e-82, "gene_start": 1, "gene_end": 126, "reference_start": 131, "reference_end": 256, "reference_sequence": "MKYAIIVDSSCALTKKQAEALGWFYTPLHINIDEKEFEDGVDINTSNLFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLIWFEQQMAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDPWVNWLAPVVAIHTGPEAFSNVAVECPKETKQIFWEKMDKIKDLL" }, "paralog": false, "identity": 0.9851851851851852, "score": 521.0, "evalue": 9.01e-191, "description": "Internal stop codon at 8095, 8185, 8194, 8587. Nonsense mutation around 8095, 8185, 8194, 8587.", "psc": { "uniref90_id": "UniRef90_C4XFL6", "product": "DegV family protein", "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL6", "UniRef:UniRef50_C4XFL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6" ], "product": "DegV family protein" } }, "id": "IGIGPGHMMC_56", "locus": "GCA000148625_00011" }, { "type": "cds", "sequence": "contig_1", "start": 8585, "stop": 8821, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTTTTACACTAAAAAAGCTAAAGTAAAAACATCGGCTATCAATATAGCTAAAACTACAGAATTATTTGAAAAATTGTCAAAAGAGTATGACAAAATTATTTTCTATCCAATCTCTCATCATTTGTCAAGTACTTATCAAACTTCAAAAACAATGGAAGAAGAGTTTCCAAAATTAAGAACAATTAAATCAGTTCAAATTGTTCAATTAATTGTTTTAGATTTAATTTGA", "aa": "MFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLI", "aa_hexdigest": "3284f02f1e1b235258535b2266f4a381", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9159.662299999996, "isoelectric_point": 9.398346138000488 }, "id": "IGIGPGHMMC_57", "locus": "GCA000148625_00012" }, { "type": "cds", "sequence": "contig_1", "start": 8932, "stop": 9627, "strand": "+", "frame": 1, "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEK0", "UniRef:UniRef90_C4XFL5" ], "nt": "ATGATGAGTCGACAATTATTGCATATTTCATATTTTATATTATATAATAAAAATATTATGGGTATATTTTTTAATTTAAATTCAAAATTTAAGAACACAAAAATCATATATTTTGATACAACAATAAACAAAAAAACTATGGATATTTATGAAGATATGGTTGTTTTTAGAGATGAAAATTTTAATGTAAATTCGGTTAATTTACTGAATGCAAAAAGTAAAAAAAGAGATGAAAAAATATTTGGTTTTTTAAATGAAAATGAAACTAAAAAACTAGTCAATAAATTGCACAAATATAACAAAAAATATTTACTAAAATCAATTAAATACTTTAGAATTGGTAAAATTGTAGAAAGAAAAAAACACCCAAAAAGCGATAGATTATTTGTATTAAAAATAGATTTTGGAAATGAAGTAAAACAAATAATAACTAACACAACATATACACTTGAAAATAAATATTTTACATTCTGTTTACCAGGATCAATTACAGCTAATGGATTAGAAATTTTAGATGGTAAAATTATGAATGTAGAAAGTGATGGAATGCTTTGCTCAGCAGAATCTTTAGGTTTAGAAGCAAAAAGCTATAAAGAATTTGAAGAAGGCTTTTTCAATAATGTTTTAGAAGAAGAAAAACAAAAATATTTAGGCAAAGAAATACAAGAATTATTGCCAGAATTAATAAGCAAATAG", "aa": "MMSRQLLHISYFILYNKNIMGIFFNLNSKFKNTKIIYFDTTINKKTMDIYEDMVVFRDENFNVNSVNLLNAKSKKRDEKIFGFLNENETKKLVNKLHKYNKKYLLKSIKYFRIGKIVERKKHPKSDRLFVLKIDFGNEVKQIITNTTYTLENKYFTFCLPGSITANGLEILDGKIMNVESDGMLCSAESLGLEAKSYKEFEEGFFNNVLEEEKQKYLGKEIQELLPELISK", "aa_hexdigest": "91e2ef31e4acc90fdf58ededd32cd344", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFL5", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.996, "score": 441.0, "evalue": 1.84e-156, "valid": true, "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "uniref50_id": "UniRef50_A0A3P8MEK0" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEK0" ], "product": "Phenylalanyl-tRNA synthetase subunit beta" }, "genes": [ "tapR" ], "id": "IGIGPGHMMC_58", "locus": "GCA000148625_00013" }, { "type": "cds", "sequence": "contig_1", "start": 9636, "stop": 10133, "strand": "+", "frame": 3, "gene": null, "product": "Tyrosine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "nt": "ATGACAATATTAGAAGATTTAAAATTAAGAGGAATATTAAAGCAAATTAGTAATGAAGATAAGTTTAAAAAACTTGATCCTAAGAAAGTTGCTGTATATTGTGGTTTTGATCCAACTGCGCAAAGTTTGCATTTAGGAAACTATGTTTTAATTTCAGTTTTAAAAAGATTTAAGCAATATGGTTTTAAAGTTTATGGACTTATTGGAGGGGCGACAGGAATGATTGGCGACCCTTCATTTAAAGATAGTGAAAGAGTTCTTTTAGATAATAAAACTTTGAAATTAAATAAAAGTAAAATTAGAAAACAATTAGAAGCTCAAGGCTTAGAAGTAATTGACAACATTCAATTTTATGAAAAAATGAATGTGCTTGAATTCTTAAGAGATGCCGGGAAACTTGTTAATGTTTCATATATGATGACTAAGGATTCAGTTCAAAAAAGAATTGAAAGAGGTTTGTCATTTACTGAATTTTCATATCAAGTTTTACAAGGTTGA", "aa": "MTILEDLKLRGILKQISNEDKFKKLDPKKVAVYCGFDPTAQSLHLGNYVLISVLKRFKQYGFKVYGLIGGATGMIGDPSFKDSERVLLDNKTLKLNKSKIRKQLEAQGLEVIDNIQFYEKMNVLEFLRDAGKLVNVSYMMTKDSVQKRIERGLSFTEFSYQVLQG", "aa_hexdigest": "32e2d6135d2d90e58316d6cf03a8d0e8", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FDW9", "query_cov": 0.9878787878787879, "subject_cov": 0.9878787878787879, "identity": 0.589, "score": 189.0, "evalue": 1.11e-58, "valid": false, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_A0A432FDW9", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FDW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "product": "Tyrosine--tRNA ligase" }, "genes": [], "id": "IGIGPGHMMC_59", "locus": "GCA000148625_00014" }, { "type": "cds", "sequence": "contig_1", "start": 10221, "stop": 10334, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTAAAGTTGAGGGTGATGATCATAAAGCTGTTGTAGTTACTGCAGACTTATTAACTGATGAAAATGGAAATAAAATTGGTAAATCAACAGGTGGTGGCAAACTTTGA", "aa": "MISKVEGDDHKAVVVTADLLTDENGNKIGKSTGGGKL", "aa_hexdigest": "c08784d71ee5ffecd1a387dce222ba13", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3798.2368000000006, "isoelectric_point": 5.527671623229979 }, "id": "IGIGPGHMMC_60", "locus": "GCA000148625_00015" }, { "type": "cds", "sequence": "contig_1", "start": 10458, "stop": 10871, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGGAAGAACATAATCAAGCACCGTTTAAACATGTAGCTCAAAGAGTAGTAGCTAGTGAAGTAGTTAGAGATATTTTTGGTCAAGATGAATTAGATTCAGCTATTCAAATTACAGAAGTTTTGTTTAACAAAAATTTTGATACAAGCAAACTAAATTTAAAAGATCTTAAATCAATTGAGCAATATTTACCAGTAGTTGAAATTAAGCAAAATCAAAATATTATTGAAACTTTAATTGAAAAAGGTTTTATTGCTTCAAAACGTGAAGCTAGAGAATTTATGGCAACTAAAGCTTTAAAAATAGATGAAGATATAGTTGAAGAAAACTCAGTTTATAAACCAAAAAACTTTTCTAAAAAGTATGCATTCTTTAGAAAAGGAAAGAAACAAACTATACTATTAAAAACTATTTAG", "aa": "MEEHNQAPFKHVAQRVVASEVVRDIFGQDELDSAIQITEVLFNKNFDTSKLNLKDLKSIEQYLPVVEIKQNQNIIETLIEKGFIASKREAREFMATKALKIDEDIVEENSVYKPKNFSKKYAFFRKGKKQTILLKTI", "aa_hexdigest": "1262a29a1076fc2da7cc82ff67a5a347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 81, "aa_cov": 0.5693430656934306, "hmm_cov": 1.0, "evalue": 4.803462205101432e-13, "score": 44.889930725097656, "start": 56, "stop": 133 } ], "seq_stats": { "molecular_weight": 15920.161699999993, "isoelectric_point": 8.70859889984131 }, "id": "IGIGPGHMMC_61", "locus": "GCA000148625_00016" }, { "type": "cds", "sequence": "contig_1", "start": 10940, "stop": 11086, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTAGTAAACCTGCTGCAAAAGGTGTAGCACCAGGAACAATTGAACCATATACTCAATTTGTTGAAGGAGCTTTAACCACTTCTCCTGTTTTTTCATCTTTTATTCCATCTTTAAAGAAGAATAATACTGTCATTTGGTAA", "aa": "MPTSKPAAKGVAPGTIEPYTQFVEGALTTSPVFSSFIPSLKKNNTVIW", "aa_hexdigest": "8b93f0389c4a074cdac943cd22818b91", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5109.8460000000005, "isoelectric_point": 9.400086784362792 }, "id": "IGIGPGHMMC_62", "locus": "GCA000148625_00017" }, { "type": "cds", "sequence": "contig_1", "start": 11101, "stop": 11277, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAATGTTCTTTATGAATTTGTTGGTACAGCTGTTTTAATTCTTATGATTTACTTAATAAACAAATCAGGATTTACTGGTTTAGGTCCTCTACCTGTAGCATTAGTTATTATGTCTATTGGTATGTCACTTGGTGGAGTAACAGGTTTTGCACTTAATCCAGCAAGAGACTGA", "aa": "MQNVLYEFVGTAVLILMIYLINKSGFTGLGPLPVALVIMSIGMSLGGVTGFALNPARD", "aa_hexdigest": "1ed2451576c322e23fea7072eb0daad2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6085.267100000003, "isoelectric_point": 5.947939109802246 }, "id": "IGIGPGHMMC_63", "locus": "GCA000148625_00018" }, { "type": "tRNA", "sequence": "contig_1", "start": 11831, "stop": 11904, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 71.4, "nt": "GCCAATTTCGCTTAGTGGTAAAGCAGCTGACTCGTAATTAGCCGCCATGAGTTCGATTCTCATAATTGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 11863, 11865 ], "id": "IGIGPGHMMC_1", "locus": "GCA000148625_00019" }, { "type": "cds", "sequence": "contig_1", "start": 12164, "stop": 12304, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAATACTCAGAATATTCATTTGAAGTTCTTTTAGGTTTTAATGTAAATGCTACAAATAATATTGTGAAGAGAATTGTTAAATCAAATTGTACTGTTGTTGTAAAGCAGTTGATTTGTATTCTTTTTTCATAA", "aa": "MVIKYSEYSFEVLLGFNVNATNNIVKRIVKSNCTVVVKQLICILFS", "aa_hexdigest": "dd281f42168a48598a9de0744da22848", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5239.2226, "isoelectric_point": 9.184440040588381 }, "id": "IGIGPGHMMC_64", "locus": "GCA000148625_00020" }, { "type": "cds", "sequence": "contig_1", "start": 12871, "stop": 12966, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATATCGGATTAAGAAAAGAAGCTAAATATGATGCAGATTTTGATGATAAAAAAATTAATGTTTATATTGACTATTTAGTTTCTTGA", "aa": "MKKHIGLRKEAKYDADFDDKKINVYIDYLVS", "aa_hexdigest": "a78ad7bcd018ab2059e676d76011e2cb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3717.251500000001, "isoelectric_point": 8.028779792785645 }, "id": "IGIGPGHMMC_65", "locus": "GCA000148625_00021" }, { "type": "cds", "sequence": "contig_1", "start": 12967, "stop": 13482, "strand": "+", "frame": 1, "gene": null, "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0", "UniRef:UniRef90_C4XFL0" ], "nt": "ATGAAAGAAAGAGTTGCTGATGCTTGTGCTAAAGGAATTGTTTTAGGTATTAGTGGAGGTATAGATTCTGCTGTAGTTGTCGCCTTAGCAAAAAAAGCTTTTGGCAAAAATGTTCTTGGTGTAGTAATGCCAGTTGATAGTATGCAAAATGATTTAAAAGATATCAAAGAATTAGAAAAAGCGGTTGATTTAAAATTTATAACTATTGATCTAAAAAATACCTATGATACTATAAAACAGGCTTTACCGTCAATTAACAACAATTTAGCTTTAAGTAATATTAAACCAAGATTGCGTATGGCTGTCCTATATGCATTAGCTCAAGAAAACAATTATTTAGTAACAGGAACAGGCAATAAATGCGAAACTCACATTGGTTATTTTACAAAATATGGCGATGGTGGAAGTGACATTTTGCCTTTAAGCAAACTGTTAAAAAGTGAAGTAAAAATTATTGCAAAGCACTTAAAAGTGCCAGACTCAATTATTAATAAAAAACCTTCTGCCGGTCTTTGA", "aa": "MKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVVMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPSINNNLALSNIKPRLRMAVLYALAQENNYLVTGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGL", "aa_hexdigest": "103814d690d5a93d60b286871960667b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 12853, "stop": 13665, "observations": { "mutation": [ 12964, 13480 ], "stop": [ 12964, 13480 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL0", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 0.971, "score": 320.0, "evalue": 4.38e-109, "gene_start": 1, "gene_end": 171, "reference_start": 39, "reference_end": 209, "reference_sequence": "MKKRGLMKKHIGLRKEAKYEADFDDKKIKAYIDYLISWMKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVIMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPLINSNLALSNIKPRLRMAVIYALAQENNYLVAGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGLWEGQNDEDELGFSYKDLDSYLMDSNAKISQEVKNKIDKMYKNSEHKRNVSLQPQEIDTILK" }, "paralog": false, "identity": 0.9481481481481482, "score": 506.0, "evalue": 1.06e-184, "description": "Internal stop codon at 12964, 13480. Nonsense mutation around 12964, 13480.", "psc": { "uniref90_id": "UniRef90_C4XFL0", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL0", "UniRef:UniRef50_C4XFL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "IGIGPGHMMC_66", "locus": "GCA000148625_00022" }, { "type": "cds", "sequence": "contig_1", "start": 13543, "stop": 13665, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTCTAATGCAAAAATTAGTCGAGAGGTCAAAAATAAAATAGACAAAATGTACAAAAATAGTGAACACAAAAGAAATGTTTCATTACAACCTCAAGAAATTGATACAATATTAAAATAA", "aa": "MDSNAKISREVKNKIDKMYKNSEHKRNVSLQPQEIDTILK", "aa_hexdigest": "2c132cb76f16e4efd08af3f0c5510199", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4730.3841, "isoelectric_point": 9.52193202972412 }, "id": "IGIGPGHMMC_67", "locus": "GCA000148625_00023" }, { "type": "cds", "sequence": "contig_1", "start": 13769, "stop": 14425, "strand": "+", "frame": 2, "gene": null, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636", "UniRef:UniRef90_A0AB32XA88" ], "nt": "ATGTTTAAAGAAATTTATGTAGCAGCAACAACAGCTGGCGGCCCAGATCCAGATATGAATCCAGCACTTAAATTAGCTATTGCAAAAGCTAAAAGTAAAAACATGCCTAAAGCAAATATTGAAAGAGCTTTAGCAAAAGTAAAAGGTGATGGTAAAGATGCATCAGCTTATACAGAAGTTTTATTCAATGCAACAATTGCAGGGGGAGCTACATTTTTAGTTGTTGCCTTAACAGATAATATGAATAGAACAACAAGTTCTATGAAAGCACACTTTAATAGACAAAATGCAGCTTTAGGTAAAACAGGTCAAGTACCTTTTGCTTTCGATCATAAAGGTGTTTTAGAAGTTTCAAAAAACTTAGTTGGAGAAGACGATCTAACTATGGTTGCACTTGAAAATGGTGCCGAAGATTTTGAAGTAACTGAAGATTCTTATATAATTACAACAGCACCAGAAAACTTTTCAAAATGTAAAAGCGCTGTTGAAAGTAACTTAAATATTAGTGATTTTATGCAATGTGAAGTTACATATATTCCTAACACAACAGTTGCTTTTGATAAAGAAAAAAGTGAAAAACTTCAAGAATTTATCGCTCGTTTAGAAGATGACGATGATGTTCAAGAAGTATTTCACAATATTGAATTTGAAGAATAA", "aa": "MFKEIYVAATTAGGPDPDMNPALKLAIAKAKSKNMPKANIERALAKVKGDGKDASAYTEVLFNATIAGGATFLVVALTDNMNRTTSSMKAHFNRQNAALGKTGQVPFAFDHKGVLEVSKNLVGEDDLTMVALENGAEDFEVTEDSYIITTAPENFSKCKSAVESNLNISDFMQCEVTYIPNTTVAFDKEKSEKLQEFIARLEDDDDVQEVFHNIEFEE", "aa_hexdigest": "d3aa5982c4ec319196bfac535b483fa3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XA88", "query_cov": 1.0, "subject_cov": 0.8685258964143426, "identity": 0.995, "score": 413.0, "evalue": 4.24e-145, "valid": true, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q4A636" }, "pscc": { "uniref50_id": "UniRef50_Q4A636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636" ], "product": "Probable transcriptional regulatory protein MS53_0373" }, "genes": [], "id": "IGIGPGHMMC_68", "locus": "GCA000148625_00024" }, { "type": "cds", "sequence": "contig_1", "start": 14458, "stop": 14766, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATTTATTAGAGCTCTTGAAAATGGTGCTTTAACAATAGGCACAAGAGAAATAGCAGCTAAATTCTATTTGATCTTAATTGCATTTGCAATTATATTTTTAGTGCCAGTTTTAGTGGCATTAATTTTTCCTTTATTTAAGAAAAGCTTAAGTAAAAAAGGAAGTATATTGCTTTATGCTTTTGTAACTGGTTTCTTTTTAACGATGGCTTTATTTGGATTTTTAAAAGAAGCTTTGGAAATAAGTTCAATTAGTGCTCCGGCATCAATGCCACCAGAAGCTTCGTCAGCGCAGATTAAATGA", "aa": "MRKFIRALENGALTIGTREIAAKFYLILIAFAIIFLVPVLVALIFPLFKKSLSKKGSILLYAFVTGFFLTMALFGFLKEALEISSISAPASMPPEASSAQIK", "aa_hexdigest": "89fbce365ff8e912c1f7d7670d63a162", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11144.412399999987, "isoelectric_point": 9.995065498352051 }, "id": "IGIGPGHMMC_69", "locus": "GCA000148625_00025" }, { "type": "cds", "sequence": "contig_1", "start": 14842, "stop": 15213, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGTTAAAAAGATAAGTGAAAAACATTTAGCTAAAAGTAAAGCAAGTGTATTTGTGCATACACATGATATGGTTCATAACCATGAACATGGTGAAAATGAAGAACATTCTCATGATCATGTGGCACAAGAACATAAAATATCAACAGACGAAAAAAATGCTAATAACAAACTAAAAGTAGTTGCATTATTGTTGTTATTAACTCACAGAATACCAGAAGGTTTCTTAATTGGTTATTCATTAAATAACTTATATAATCAGAATATTGGCTCATTAAGTATTGCATTCTTTGTTTCATTTATAATGCACTTAATACCTGAAGAAATGGTTTTTTATTATAGACAACGTGAAATGGGTGTAGGTCGTTGA", "aa": "MTVKKISEKHLAKSKASVFVHTHDMVHNHEHGENEEHSHDHVAQEHKISTDEKNANNKLKVVALLLLLTHRIPEGFLIGYSLNNLYNQNIGSLSIAFFVSFIMHLIPEEMVFYYRQREMGVGR", "aa_hexdigest": "9c001a56dc7c38a9eb214bd163165308", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14216.102099999991, "isoelectric_point": 6.864524650573731 }, "id": "IGIGPGHMMC_70", "locus": "GCA000148625_00026" }, { "type": "cds", "sequence": "contig_1", "start": 15331, "stop": 15498, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGCTGGTATATTCTTCTTTACAGCTGTGATGGAATTTCTACCTGAGTTTTATCATGCACATCATGACAAAGATTTGTTCAAAAAAGTTATGATTACATTTATGGCTGGACTTGTGGTTTGTGCCTTGATATTAGCATTGCATCAACATGGGACATATAATTAA", "aa": "MIAGIFFFTAVMEFLPEFYHAHHDKDLFKKVMITFMAGLVVCALILALHQHGTYN", "aa_hexdigest": "a8919743e0d3618869803bc5ad767110", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.501399999999, "isoelectric_point": 6.476882743835449 }, "id": "IGIGPGHMMC_71", "locus": "GCA000148625_00027" }, { "type": "cds", "sequence": "contig_1", "start": 15692, "stop": 16096, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAGATTTGTTAACGTTGTAGATTTACTTAAATTAAGACATCCAAAAATTGACCCACGCGGTTTAAGTGATGAAGAATTTGATGAAATCTTTACAAAAGACAAACCAGTATTATTTGCTTTCCATGGTTTTGAAGGTATTTTAAGAGACATCTTCTTTGACCGTCACAACCACAATTTAATTGCTCACGGTTATAGAGAAAATGGTGACATTACAACATCATTCGACATTCGTCAATTAAGTCACATGGACAGATTCCACATGGCTAGCGATGCTGCTGCAGCTGTATTCGGATCATCAAAAGCAAAAGAATTCATGGACAAAATGGAAGAAACAATCCAATTCCACAACAAATACATTCGTGAAGTTGGTACAGATATTCCTGAAGTTAAAAACTGA", "aa": "MKIRFVNVVDLLKLRHPKIDPRGLSDEEFDEIFTKDKPVLFAFHGFEGILRDIFFDRHNHNLIAHGYRENGDITTSFDIRQLSHMDRFHMASDAAAAVFGSSKAKEFMDKMEETIQFHNKYIREVGTDIPEVKN", "aa_hexdigest": "841dfb96fb36c35c9497b9abd1ba2ec1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 1.0, "subject_cov": 0.8072289156626506, "identity": 0.657, "score": 192.0, "evalue": 2.08e-60, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15654.597799999987, "isoelectric_point": 6.0416666030883786 }, "id": "IGIGPGHMMC_72", "locus": "GCA000148625_00028" }, { "type": "cds", "sequence": "contig_1", "start": 16211, "stop": 16480, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACTTCAACTGCTTTCCAACAAGACCACAATGGTTATACACACCAAGACCCAGGTATTATTGGACACATGGCAGATAAAAGACCAGAACTAATTAGAGAATACTTACCAGCTGATACAAACACATTATTAGCCGTTATGGATAAAGCATTTACAGAAAGAAATGTAATTAACTTAATCGTTTCTTCAAAACAACCAAGACACCAATTCTACTCAGTAGAAGAAGCTGAAACATTGGTTGAAAAAGGTTTAGACATTATTGATTGA", "aa": "MATSTAFQQDHNGYTHQDPGIIGHMADKRPELIREYLPADTNTLLAVMDKAFTERNVINLIVSSKQPRHQFYSVEEAETLVEKGLDIID", "aa_hexdigest": "74af9989c280f6b35af6fe415926b4db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10085.208899999996, "isoelectric_point": 5.021747779846191 }, "id": "IGIGPGHMMC_73", "locus": "GCA000148625_00029" }, { "type": "cds", "sequence": "contig_1", "start": 16535, "stop": 16726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAATTGATTCTAAATTAGATGAAGCTCTTAGTCCAGTAGGTAGAATCATTGACTCACAACTTTCAGAACACCAAGCTCAAGGTTTCTTAGAAGGTTATGTATTAACAGGTCGTCATGGTATCTTTGCTTCATATGAATCATTCTTAAGAGTTGTTGACTCAATGGTTACACAACACATGAAATGA", "aa": "MERIDSKLDEALSPVGRIIDSQLSEHQAQGFLEGYVLTGRHGIFASYESFLRVVDSMVTQHMK", "aa_hexdigest": "956d56023b3219d2e85c34ae7d915f9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7124.998099999999, "isoelectric_point": 5.415187263488768 }, "id": "IGIGPGHMMC_74", "locus": "GCA000148625_00030" }, { "type": "cds", "sequence": "contig_1", "start": 16727, "stop": 16855, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAATTAGGAACATACTTTGCTGACTTAGTAAAAAGAAATCCAGAAAACTTTAGAATTTTTGGACCAGATGAAACTAAATCAAACCGTCTTTACAACTTATTCAAAGTTACAAACCGTCAATGA", "aa": "MVELGTYFADLVKRNPENFRIFGPDETKSNRLYNLFKVTNRQ", "aa_hexdigest": "214a769cc8436e2b93a27ee96b92b44a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5022.652500000001, "isoelectric_point": 9.394735908508299 }, "id": "IGIGPGHMMC_75", "locus": "GCA000148625_00031" }, { "type": "cds", "sequence": "contig_1", "start": 16962, "stop": 17258, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCATTCTTCTTTTGCCTTTAGGAGCAAGAGCAGCGATTTCTTTTACCAATTTACCTTTTTTATCAAATAATTCTTCAGGACGATATGATCTTAATCATTTTTCAAGTGCATCTGCATGTTCCATGTTTTCTGCATTAATTGGAAGTGGAACTTGGTGTGCACGGAAGCTTCCTTCAATAGCTTCTTTGTTTCATGATTTAGGACCTGTTCAACCTTTAGGTGTTCTAAGAACGATCATTGGTCATACTGGTCTTTTAGCTTTGTCAGCTGGTTTTTTACGAGCTTTTGTTTGA", "aa": "MPILLLPLGARAAISFTNLPFLSNNSSGRYDLNHFSSASACSMFSALIGSGTWCARKLPSIASLFHDLGPVQPLGVLRTIIGHTGLLALSAGFLRAFV", "aa_hexdigest": "7abd13c71dac5d51f953662d79936b1d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10315.97399999999, "isoelectric_point": 10.082097816467282 }, "id": "IGIGPGHMMC_76", "locus": "GCA000148625_00032" }, { "type": "cds", "sequence": "contig_1", "start": 17207, "stop": 17314, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTAATGGCAAAAACTTTAGACTCAGTTATTGAAGAAATTCAAAGCATTCAAACAAAAGCTCGTAAAAAACCAGCTGACAAAGCTAAAAGACCAGTATGA", "aa": "MHELMAKTLDSVIEEIQSIQTKARKKPADKAKRPV", "aa_hexdigest": "03a2515dbd7b44d45134cf5fd3f995f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3991.6814000000013, "isoelectric_point": 9.696447944641115 }, "id": "IGIGPGHMMC_77", "locus": "GCA000148625_00033" }, { "type": "cds", "sequence": "contig_1", "start": 17495, "stop": 17812, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTACATTGAAGGACCAGGACATGGTGGCCAAGTTATGACATCTAACTCATACTTAGATGGAAGTTACACAGAATTATTCCCACATGTAACACAAGATGAAGCCGGAATGCAACATTTATTTAAATACTTCTCATTCCCTGGTGGAACAGCTTCACATGCTGCTCCTGAAACCCCTGGATCAATTCATGAAGGTGGAGAATTAGGTTACTCAATTTCACACGCAACAGGTGCAATTTTAGATAACCCTGATGTTATTGCTGCTACTATCGTTGGTGATGGTGAAGCTGAAACAGGACCTCTTGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMTSNSYLDGSYTELFPHVTQDEAGMQHLFKYFSFPGGTASHAAPETPGSIHEGGELGYSISHATGAILDNPDVIAATIVGDGEAETGPLATS", "aa_hexdigest": "abaf62c10f830fc60a38ce8589f21cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10886.74039999999, "isoelectric_point": 4.366280555725097 }, "id": "IGIGPGHMMC_78", "locus": "GCA000148625_00034" }, { "type": "cds", "sequence": "contig_1", "start": 18283, "stop": 18600, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACAAACCAAATTTCAGTTGTTCCTAAACCTGCTAATGTTGCAGTTAGTGACAAACTGTACATGCTTGAGCCTTTAGCTCAAATTACTGAAAAGGATTTAGAATTCTTTAACTCAAAAGATTTTAGACACTTTTATCAAATAGCCACAAACTTTTCATCAAGATCAAGCTCAGTTGATACATCAAGTGTATTCTACTTCGGTTTGCTTCAAGAAGATGATGTTGAAACAAACAAAAAACAAAGCAAAACTCAAGAATACGATTTCTCAGATGACTTTGATATTGACACCAAAGAATACGAAAGCAGTTTCTAA", "aa": "MNTNQISVVPKPANVAVSDKLYMLEPLAQITEKDLEFFNSKDFRHFYQIATNFSSRSSSVDTSSVFYFGLLQEDDVETNKKQSKTQEYDFSDDFDIDTKEYESSF", "aa_hexdigest": "8231ea5d23ae32567c3a0fec4bbcdbb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12180.175599999991, "isoelectric_point": 4.3731580734252935 }, "id": "IGIGPGHMMC_79", "locus": "GCA000148625_00035" }, { "type": "cds", "sequence": "contig_1", "start": 19135, "stop": 19725, "strand": "-", "frame": 2, "gene": null, "product": "DNA methylase adenine-specific domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "nt": "ATGTTTGGTTCACTAACTGAAAGAAACATAAAAGATATCATCAAAAAAGAAAAATTAACAGATGTTAAATATTTAATTAAATATATTGAAAAAAATCCAAAACTTATGGATGTTAGATCAATGTACCAAGTAGCTCAATATGCAAACTGCTTGAGATTAGGTCAAAAACCTTTTTATACAGATGAAAGTTTACTAAACTCAATTTACAAAAGACTTCCAAATATTAGAGGCAAAGAATTAACGATTGTTGATCCTTTATGTGGTGTTGGTATTTTCTTACCTTTAATTATTGAAAAATATGGAGACAAAAACAAACTAAGTATTACTTTTTATGATGTAGATAATGAAACAATAAATATTGCTCGCGCTTTATCTAAAATGATAAAACTTAAAGTTACTTTTGGTGTTAGTTTTCAAAATGCTGATTTTCTAATGAAAAGAACTAATGAACCAATTGACTTATTAATTGGTGATTTCCCTAACATAAGAATGTCTCATCCAGCTTTAAGAAAAAAATATCCTTCATTAACTAAAGAAAACAAATTATTACAAAGTTTTGCTCCATTATACTTAATGCAAAGTAAAAAATGA", "aa": "MFGSLTERNIKDIIKKEKLTDVKYLIKYIEKNPKLMDVRSMYQVAQYANCLRLGQKPFYTDESLLNSIYKRLPNIRGKELTIVDPLCGVGIFLPLIIEKYGDKNKLSITFYDVDNETINIARALSKMIKLKVTFGVSFQNADFLMKRTNEPIDLLIGDFPNIRMSHPALRKKYPSLTKENKLLQSFAPLYLMQSKK", "aa_hexdigest": "fd069417c629aba541226571130edc6a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A3P8L726", "query_cov": 0.8775510204081632, "subject_cov": 0.9398907103825137, "identity": 0.8490000000000001, "score": 295.0, "evalue": 6.72e-100, "valid": false, "product": "DNA methylase adenine-specific domain-containing protein", "uniref50_id": "UniRef50_A0A3P8L726" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L726", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "product": "DNA methylase adenine-specific domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_80", "locus": "GCA000148625_00036" }, { "type": "tRNA", "sequence": "contig_1", "start": 20016, "stop": 20099, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 20022, 20024 ], "id": "IGIGPGHMMC_2", "locus": "GCA000148625_00037" }, { "type": "tRNA", "sequence": "contig_1", "start": 20103, "stop": 20177, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 64.1, "nt": "TGCCCCATAGCCAAGCGGTAAGGCAACGGGTTTTGGTTCCGTCATGCGTTAGTTCGAATCTAACTGGGGCAACCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 20135, 20137 ], "id": "IGIGPGHMMC_3", "locus": "GCA000148625_00038" }, { "type": "cds", "sequence": "contig_1", "start": 20475, "stop": 20597, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAGGACCTAGAATGCATATAGGTGTATCCGTACCTATGGTTATGATCATAATGATGGGATTTGGTCTATTTGCGCTTTCTATTCCTCTTTACTATGCAAAAGAAACCATGGTTTGA", "aa": "MFLGPRMHIGVSVPMVMIIMMGFGLFALSIPLYYAKETMV", "aa_hexdigest": "833f39d305f37793f8aeacf3ad227b79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4493.616300000001, "isoelectric_point": 8.274855613708496 }, "id": "IGIGPGHMMC_81", "locus": "GCA000148625_00039" }, { "type": "cds", "sequence": "contig_1", "start": 20868, "stop": 20993, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATGAAGAAACTAAAAAAGAAATGATAAAATTATCTCTTTTCTTTGGTTTCAAATTAGCATATGATTATATTTATTGTGTACTTTATTTAATTAGATTATTCTCAAATATGAAAAATTAA", "aa": "MDDEETKKEMIKLSLFFGFKLAYDYIYCVLYLIRLFSNMKN", "aa_hexdigest": "fbbbccb6dc9e5d3d3ffbe843ee3a51bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5034.9279, "isoelectric_point": 6.057411003112793 }, "id": "IGIGPGHMMC_82", "locus": "GCA000148625_00040" }, { "type": "cds", "sequence": "contig_1", "start": 21308, "stop": 21787, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4", "UniRef:UniRef90_C4XFK4" ], "nt": "ATGAATAAAAAATTTTTAAAATTAGGCTCAATTGCAGGAATTTTATCTTTTGCACCAGTAGCAATTAGTGCTGGATGTGGTTCTAAAGGTGGAGAAACACCTACTGATTCACTTGCTAATAAGAAGGCATTAGTTGAACAAGCATTAAAAGATTTAAATGCTAAAATTGAAACTGTTGCTGATGGAACTAAAAAAGCTGAACTTAAAAAGGAAGTAGAAGCTATTAAAAAAGATTTTGATGCCGCTAAAACAATTAAAGATTTTGAAGCTGTAGATGCAAAAATTAAAAAAGTTGCTGCTAAAGTTGAAAGCGGAAGTACATCTGAACAAGACAAATATAAAACATGCTTTACTTTAGGAGAAGGCAAAGATAGAATTAAAGAAGCTATTGAAGCCATAAAAAAAGATTCTAAATCATATTCATTTGCATATGATCGTAAAGGCCACAACATTGTTTGCTTTAAAGGACAAATTAATTGA", "aa": "MNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDSLANKKALVEQALKDLNAKIETVADGTKKAELKKEVEAIKKDFDAAKTIKDFEAVDAKIKKVAAKVESGSTSEQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKGHNIVCFKGQIN", "aa_hexdigest": "5009ea853b8a4dbd6acb292b86550ed1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 21053, "stop": 21814, "observations": { "mutation": [ 21310 ], "stop": [ 21310 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK4", "query_cov": 1.0, "subject_cov": 0.6284584980237155, "identity": 0.9309999999999999, "score": 280.0, "evalue": 1.44e-93, "gene_start": 1, "gene_end": 159, "reference_start": 10, "reference_end": 168, "reference_sequence": "MVKIMRRKSMNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDPVAAKKALVEQALKDLNAKIETVTDETKKAELKKEAEAIKKDFDAAKTVKDFEAVDAKIKKVVAKVESKSTSDQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKGHNIVCFKGRINWSNGDKVIALNITGALKEHIEKNSVQLANAKKPTYNSKKGESISSILDFKLEGNTVKFSFKLAKFENNKPVLDDPIVYNAEVSLA" }, "paralog": false, "identity": 0.924901185770751, "score": 457.0, "evalue": 1.1e-165, "description": "Internal stop codon at 21310. Nonsense mutation around 21310.", "psc": { "uniref90_id": "UniRef90_C4XFK4", "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK4", "UniRef:UniRef50_C4XFK4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17151.55669999999, "isoelectric_point": 9.27237491607666 }, "id": "IGIGPGHMMC_83", "locus": "GCA000148625_00041" }, { "type": "tRNA", "sequence": "contig_1", "start": 21870, "stop": 21947, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 70.4, "nt": "GTCCTTGTAGCTCAGATGGATCAGAGCACAAGCCTCCTAAGCTTGGGGTGGGTGGTTCAATTCCACTCAAGGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 21943, 21945 ], "id": "IGIGPGHMMC_36", "locus": "GCA000148625_00042" }, { "type": "tRNA", "sequence": "contig_1", "start": 21986, "stop": 22062, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 64.3, "nt": "GGTCGCGTAGCTCAGTAGGATAGAGCACGAGCCTTCTAAGCCCGTGGTCAGAGGTTCGAATCCTCTCGTGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 22026, 22028 ], "id": "IGIGPGHMMC_35", "locus": "GCA000148625_00043" }, { "type": "cds", "sequence": "contig_1", "start": 22782, "stop": 22913, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCR3", "UniRef:UniRef90_A0AB32XCR3" ], "nt": "ATGAAAAGAAAATCAATAAATCCTATTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAATGA", "aa": "MKRKSINPIIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQK", "aa_hexdigest": "5c85b0624755f6876ef8a01f872abd74", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0AB32XCR3", "query_cov": 1.0, "subject_cov": 0.86, "identity": 0.953, "score": 85.1, "evalue": 3.73e-21, "valid": true, "product": "Transposase", "uniref50_id": "UniRef50_A0AB32XCR3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCR3" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_84", "locus": "GCA000148625_00044" }, { "type": "cds", "sequence": "contig_1", "start": 22910, "stop": 23023, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTTAA", "aa": "MMNKEFDNFMGYEKNDNKVQKNYRNGFSKKNVNSQYG", "aa_hexdigest": "c956e06b3ccc286abb0834ef151e527c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4468.916300000003, "isoelectric_point": 9.456109809875489 }, "id": "IGIGPGHMMC_85", "locus": "GCA000148625_00045" }, { "type": "cds", "sequence": "contig_1", "start": 23024, "stop": 23209, "strand": "+", "frame": 2, "gene": null, "product": "transposase", "db_xrefs": [ "RefSeq:WP_258408853.1", "SO:0001217", "UniParc:UPI00030AFE14", "UniRef:UniRef100_UPI00030AFE14", "UniRef:UniRef50_UPI00030AFE14", "UniRef:UniRef90_UPI00030AFE14" ], "nt": "ATGGAAATTGACATTCCAAGAGATCATGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAAAGATGTAAGTGATTTTTGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAA", "aa": "MEIDIPRDHEAKFEPIIIKKYERDISELVDMVFALYSRGMSTKDVSDFCLANMALIILQHK", "aa_hexdigest": "17dbf00b1b0ba7d9088f81bac88fe08e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00030AFE14", "ncbi_nrp_id": "WP_258408853.1", "uniref100_id": "UniRef100_UPI00030AFE14", "db_xrefs": [ "SO:0001217", "UniParc:UPI00030AFE14", "RefSeq:WP_258408853.1", "UniRef:UniRef100_UPI00030AFE14" ] }, "ips": { "uniref100_id": "UniRef100_UPI00030AFE14", "uniref90_id": "UniRef90_UPI00030AFE14" }, "psc": { "uniref90_id": "UniRef90_UPI00030AFE14", "product": "transposase", "uniref50_id": "UniRef50_UPI00030AFE14" }, "pscc": { "uniref50_id": "UniRef50_UPI00030AFE14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030AFE14" ], "product": "transposase" }, "genes": [], "id": "IGIGPGHMMC_86", "locus": "GCA000148625_00046" }, { "type": "cds", "sequence": "contig_1", "start": 23431, "stop": 23697, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATACCCTTAAAAATATAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCCATTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATTTGCATATCAGTCTAATGATACAAAAAATGCAATGGATAATTTGGATATTTTTGAAAATAAATGA", "aa": "MSVLNTLKNIGISKINIICSDNLKGIQQSIEAIFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKFAYQSNDTKNAMDNLDIFENK", "aa_hexdigest": "f29ccf44a123fccbb720f2bed0b0998e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 43, "aa_cov": 0.4772727272727273, "hmm_cov": 1.0, "evalue": 1.178687639634669e-07, "score": 28.167224884033203, "start": 8, "stop": 49 } ], "seq_stats": { "molecular_weight": 10207.720599999991, "isoelectric_point": 8.80704212188721 }, "id": "IGIGPGHMMC_87", "locus": "GCA000148625_00047" }, { "type": "cds", "sequence": "contig_1", "start": 24407, "stop": 24757, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACTGTTAAAACTTCTTACCCTGATTTATTAATAAAATATAAAAATCACCAAATACTTCTCGAAATAAAAGGCCTAAAAGACATAGATAAAGATAAAACCCTTAAAATGTTAAGAAATTATAAACAATACACAAGTGATGATATTATCGAAAAATTGGATAAAAACAGCGATTCCTTAACTATTGGTGTTGTTCATGTATCAAATAATAGACAAACATTAATCGCGTATAGTACACTAAAACATGTAAATGATTATTATTCTAAAACAGATGATCCTGACAAAGCAAAAGATTTTGATTTTGGAAAATTTTTTGATATTTTAGAGTATTACGATAAAAACGATTAG", "aa": "MNTVKTSYPDLLIKYKNHQILLEIKGLKDIDKDKTLKMLRNYKQYTSDDIIEKLDKNSDSLTIGVVHVSNNRQTLIAYSTLKHVNDYYSKTDDPDKAKDFDFGKFFDILEYYDKND", "aa_hexdigest": "d0d9b65a383ae0a1464de14f25799123", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13679.292899999982, "isoelectric_point": 6.27913990020752 }, "id": "IGIGPGHMMC_88", "locus": "GCA000148625_00048" }, { "type": "cds", "sequence": "contig_1", "start": 24809, "stop": 25036, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTTGACGATTATCAATATGTTTCTAATTCAAATAACTGCTTAAAATTTAATCAATCGCCATCATTTGATGAATATTTTTTAAAATATGCGTATTTAAATACAAACAAAAAAAATGAAGGTATTCACCATTTGGATAGTAAAAATGAAGTTCAAGTTTTAGGGAATTTCAAAAATTTATTTTTTAATAGTCAGCTTAATTTAAAAGATAAAATCAATATTTGA", "aa": "MPFDDYQYVSNSNNCLKFNQSPSFDEYFLKYAYLNTNKKNEGIHHLDSKNEVQVLGNFKNLFFNSQLNLKDKINI", "aa_hexdigest": "8dceaa218baf819f87ab5c320d583f0c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8901.828599999995, "isoelectric_point": 6.702760887145996 }, "id": "IGIGPGHMMC_89", "locus": "GCA000148625_00049" }, { "type": "cds", "sequence": "contig_1", "start": 25136, "stop": 25420, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTATTATTAGACAATAAAAATTATAAAAACATCTATATCGAAAAAGGATTCATCGAAAATAAAATACAAGAACTTTATAATGATAATTACAACTTCAAAATAATTGAAAGTATAATACAAAACAAAATTGAACTAAGAATAGAAATAATGAAACTTAAAAATAGTTTAAAAAGATTTCTAAACAAAAAAGTTAATGAATTAATTCAATCAAAATTTGACGAATTTAACAAGAGCATAATTGATGACTTAAAAACTAAAATAGATAATGATATTTTATGA", "aa": "MDLLLDNKNYKNIYIEKGFIENKIQELYNDNYNFKIIESIIQNKIELRIEIMKLKNSLKRFLNKKVNELIQSKFDEFNKSIIDDLKTKIDNDIL", "aa_hexdigest": "deafd5cd832d7385d2f9594b3ab6701a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11386.108199999982, "isoelectric_point": 7.840403175354004 }, "id": "IGIGPGHMMC_90", "locus": "GCA000148625_00050" }, { "type": "cds", "sequence": "contig_1", "start": 25577, "stop": 25741, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTTCAATTAAGAAATGTTTCATCAGATAGTTTAAATGTTCAAACAGTTGGTAGAATAAAAAGAAATCCTGCACCAGTTGAAAAATCTTTTGAAAATATAAATTCTATTAAATTTAATTATTATTTATATTCAAACTTCAAAGATGAAAAACGTTTATGA", "aa": "MLVQLRNVSSDSLNVQTVGRIKRNPAPVEKSFENINSIKFNYYLYSNFKDEKRL", "aa_hexdigest": "b3107ac9ac0bbea399ebc5344c216ed7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6365.193099999999, "isoelectric_point": 9.773100852966305 }, "id": "IGIGPGHMMC_91", "locus": "GCA000148625_00051" }, { "type": "cds", "sequence": "contig_1", "start": 25895, "stop": 26803, "strand": "-", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C1293D2" ], "nt": "ATGCAATTAACACAAAGTCAACAAAAAGCTGTTAATGAATTGTTAGAATCTTTTAATCCAAACAAAAAAATACAAGTATATTTTAGCGCTCCAACAGGAAGTGGAAAGACTTTTATGATTTCTAATTTTATTAATGATTTAGCTAAAAATTATTATAATGAAAAACTTATATTTATTATTGCTACACTCTCTTCAAGTGAATTGCCTAAACAAATGGAAAATAATTTAAATGAATATAAAATGTTTTTAGATTATTCTTCAAATTTAAAAATAGAAAGAATAATTTCCCCAAGTGTTAAAAATTTAAAAAGTAAAGATCAAGATGTTTCTATGATTGCTGAAAAAAATAAAGTTATAATCTTGGGAAATGCAAGTTTCAAAAAAAATTGTATTCTGGTTGAAAGAGGAAAATTGGAAAGTTTTTTACAACAAATAAAAAACGAAAATTATAAATTGATTTATATCAGAGATGAAGCTCATCATGGTGGAAAAGTTGAAAAAAACAAAGATGATAGTGGATTTGAAACAAAAATGCAAAAAATTGCACATTATATTATTAAAATGACTGCAACCCCTAAAGAATACAACATAAAACAAATAAGAATCGATGAAGACGATTTGAAAAAAGATAACATATATTTATTGAAACAATGTCCTGTTTTTAATGATTTGAAAGACTATGAAAAAGAAGAATACACAAATGATGAAATTTTAGATATTGCATGTAAAAAATTTAAAGCAATTAAAAAAGAATATATGGATTCTGAAAAAGAACCTTCTTTAATCGGAATTAACCCTGCTATGTTAATACAAGTAAAAGACGAACCTAAAGATCCACATTTGAAAAAAGAATTTAAAAACAATATTGATTCAATAATAAAAATACTAGAGAATAATGGTCTTACGTGA", "aa": "MQLTQSQQKAVNELLESFNPNKKIQVYFSAPTGSGKTFMISNFINDLAKNYYNEKLIFIIATLSSSELPKQMENNLNEYKMFLDYSSNLKIERIISPSVKNLKSKDQDVSMIAEKNKVIILGNASFKKNCILVERGKLESFLQQIKNENYKLIYIRDEAHHGGKVEKNKDDSGFETKMQKIAHYIIKMTATPKEYNIKQIRIDEDDLKKDNIYLLKQCPVFNDLKDYEKEEYTNDEILDIACKKFKAIKKEYMDSEKEPSLIGINPAMLIQVKDEPKDPHLKKEFKNNIDSIIKILENNGLT", "aa_hexdigest": "b7f248da2f135b0b0ad063795cc5a9ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001C1293D2", "query_cov": 0.9470198675496688, "subject_cov": 0.8854489164086687, "identity": 0.524, "score": 238.0, "evalue": 6.67e-74, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001C1293D2" }, "pscc": { "uniref50_id": "UniRef50_UPI001C1293D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C1293D2" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "IGIGPGHMMC_92", "locus": "GCA000148625_00052" }, { "type": "cds", "sequence": "contig_1", "start": 26803, "stop": 26925, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAATTTATGAAAAAGAATTAAAAGATTTTAATGTAAAAATTAACAAAAATACTGATATAAGTACATTCTACTATGATTTACTTGCTTTAAAACCAATAGAAAAAAGTGAAGGTAAATAA", "aa": "MEIYEKELKDFNVKINKNTDISTFYYDLLALKPIEKSEGK", "aa_hexdigest": "b97dd3b0cc24b5914f4ae632fc57d21d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4769.4232, "isoelectric_point": 5.179873847961424 }, "id": "IGIGPGHMMC_93", "locus": "GCA000148625_00053" }, { "type": "cds", "sequence": "contig_1", "start": 27025, "stop": 27372, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01555.25" ], "nt": "ATGGGCCCAAAATTAAATACAAAAAATGGTAATGATCTTTTAGAAAATATTTGTGAAAATATAGAATTAGATGAAAATACCAAATTTAACACTTGTAAAAATATAGATTTAATAAATTATTTAATAAATATTAAATTGAATAATAAAGAAGCCAGAATTTTAGATTTTTTTGCTGGCTCAGGAACTACAGGACATGCAATTTTAGAAATGAACAAAAAGGATGGCGGTAAAAGACAGTTTACGCTTGTTACAAATGACGAAAACAATGTAGGAACAAAAATTTGCTATGAGAGATTATATAGAATCAATAATGGCAAAGGCACTGATAATAGTACTTCTTTTGCATGA", "aa": "MGPKLNTKNGNDLLENICENIELDENTKFNTCKNIDLINYLINIKLNNKEARILDFFAGSGTTGHAILEMNKKDGGKRQFTLVTNDENNVGTKICYERLYRINNGKGTDNSTSFA", "aa_hexdigest": "dc4af8748f6097a03b2d9e12322d4753", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01555.25", "name": "N6_N4_Mtase", "length": 53, "aa_cov": 0.43478260869565216, "hmm_cov": 0.9811320754716981, "evalue": 4.8839555827283665e-11, "score": 38.99933624267578, "start": 24, "stop": 73 } ], "seq_stats": { "molecular_weight": 12920.386699999981, "isoelectric_point": 6.569530296325683 }, "id": "IGIGPGHMMC_94", "locus": "GCA000148625_00054" }, { "type": "cds", "sequence": "contig_1", "start": 27493, "stop": 28011, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01555.25" ], "nt": "ATGCTTAATGAAAGACTAATAATGGCTCGCGATCTTTTAAAAGAAGATGGTGTTATTTTTGTGTCAATTGATGACATTGAACAAGCTTATTTAAAAATCTTAATGGATCAAATATTTGGAGAAGAGAATTTTATAGCTTGTATGCCATACATTTCAAACTTAAAAGGAAGACAAGCAAACACAAATTTTGCATTAACACATGAATATATCTTATGTTATTCAAAAAGTAATTCTTTTGAATTCCAAGAATTAGAAGACAAATATGCAACAGAATTAATGCCTCAAATATATAAAGAAAGAAATAAAAAAATCTTTAAAGATACATATGGCTACTTTATATTACAAAATAGGTTAGAAAATACAAACAGCAAGTTTAACAAACAAACAAGAAGCAACTTGTATTATCCTTTATACATATATAAGGATAGGAACGAATATAGAGTTGATGTTGTAAAAACCGAAAAAACTATTGCAGAAATTTTTCCTAAAAAAATTAAAAATATTTATGACGGTGTTTGA", "aa": "MLNERLIMARDLLKEDGVIFVSIDDIEQAYLKILMDQIFGEENFIACMPYISNLKGRQANTNFALTHEYILCYSKSNSFEFQELEDKYATELMPQIYKERNKKIFKDTYGYFILQNRLENTNSKFNKQTRSNLYYPLYIYKDRNEYRVDVVKTEKTIAEIFPKKIKNIYDGV", "aa_hexdigest": "817a9dad3e913891079b995dea7ffc0c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01555.25", "name": "N6_N4_Mtase", "length": 127, "aa_cov": 0.6976744186046512, "hmm_cov": 0.9921259842519685, "evalue": 9.00250098076612e-15, "score": 51.210235595703125, "start": 2, "stop": 121 } ], "seq_stats": { "molecular_weight": 20589.38680000001, "isoelectric_point": 6.6194349288940435 }, "id": "IGIGPGHMMC_95", "locus": "GCA000148625_00055" }, { "type": "cds", "sequence": "contig_1", "start": 28160, "stop": 28528, "strand": "-", "frame": 1, "gene": null, "product": "Type III restriction-modification system: methylase", "db_xrefs": [ "RefSeq:WP_013354297.1", "SO:0001217", "UniParc:UPI0001E32EDF", "UniRef:UniRef100_UPI0001E32EDF", "UniRef:UniRef50_A0A2C9DYR5", "UniRef:UniRef90_UPI0001E32EDF" ], "nt": "ATGAAAGAACATAAAAGTAAAAATGAAGAATTAAGAGCTTCATACAATAAAAAAATAGATGAACTTGCGAAAGCTGAAGTTAATCAAGATCAAAAAGATTTATTTAAAAAAATATTAGAAAAAGCAGATGATGCAGATTTACAAAATATATATCAAATGTTAATTCAAAGAGTTAAAGTTGGATTTACTTTTGATGCAGCACCATCTGTAGATCAAAAAGCTGTTGCTTTACTTGTTAAAGATGAAGAAAAATCATTTATTAATGACAAAAAGAATATCGATAATCAAAACATTTTAATCATAGGTGAAAATTATGACGCTTTAAAGAATCTTTTAATAGTCGAGAGAGAGAGAGAAGAATGGGACTAA", "aa": "MKEHKSKNEELRASYNKKIDELAKAEVNQDQKDLFKKILEKADDADLQNIYQMLIQRVKVGFTFDAAPSVDQKAVALLVKDEEKSFINDKKNIDNQNILIIGENYDALKNLLIVEREREEWD", "aa_hexdigest": "8e5b8581af542d263a70647b963b1df6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32EDF", "ncbi_nrp_id": "WP_013354297.1", "uniref100_id": "UniRef100_UPI0001E32EDF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EDF", "RefSeq:WP_013354297.1", "UniRef:UniRef100_UPI0001E32EDF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32EDF", "uniref90_id": "UniRef90_UPI0001E32EDF" }, "psc": { "uniref90_id": "UniRef90_UPI0001E32EDF", "uniref50_id": "UniRef50_A0A2C9DYR5" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYR5" ], "product": "Type III restriction-modification system: methylase" }, "genes": [], "id": "IGIGPGHMMC_96", "locus": "GCA000148625_00056" }, { "type": "cds", "sequence": "contig_1", "start": 28663, "stop": 28989, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATACACAGAACAAGAACAAATTCGTCGTGATAAACTAGAATTTTATAAAAAATTTGATGTAGTACCTTTTAAAAAAGCTTATGGTCTAGGTAAATTGAGTACAAGTGAAGAACTCAATAAAAAATATGGTCAGTTTACTAGAGAAGAATTAGAAGTAAAAAAAGTTAAGAAAAATATTAGTGGCAGATTAATTACAGCTAGGGGGCCTTTCTTAGTATTAAAAGATCGTAAAGGAACAATTCAAGTTTATTTCAATAAAAAAGAAAATCCTGAATTAACTAAAATAGTTGAAACATTTGACTTAGGAGACATTTTATGA", "aa": "MAKYTEQEQIRRDKLEFYKKFDVVPFKKAYGLGKLSTSEELNKKYGQFTREELEVKKVKKNISGRLITARGPFLVLKDRKGTIQVYFNKKENPELTKIVETFDLGDIL", "aa_hexdigest": "3c40e144bbe5a5270bccf78b66890bb8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.621499999983, "isoelectric_point": 9.638877677917481 }, "id": "IGIGPGHMMC_97", "locus": "GCA000148625_00057" }, { "type": "cds", "sequence": "contig_1", "start": 29005, "stop": 29199, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "nt": "ATGAAAACTCATACAGGTGAAATGACAGTTAAGGCTCAAGATATTCAATTGTTAACTAAAGCTCTTAAACCTCTTCCAGATAAATTTCATGGATTAACTGATACTGAAGAAAGATATCGTCACAGATACTTAGATTTAATTACTAATCCAGAATCAAGAAATACATTCATTATGAGAACAAAAATTGTTCAATGA", "aa": "MKTHTGEMTVKAQDIQLLTKALKPLPDKFHGLTDTEERYRHRYLDLITNPESRNTFIMRTKIVQ", "aa_hexdigest": "a05bcde9c623552004515b4da0aca41b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00257604EB", "query_cov": 0.96875, "subject_cov": 0.8378378378378378, "identity": 0.629, "score": 85.5, "evalue": 1.17e-20, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00257604EB" }, "pscc": { "uniref50_id": "UniRef50_UPI00257604EB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "IGIGPGHMMC_98", "locus": "GCA000148625_00058" }, { "type": "cds", "sequence": "contig_1", "start": 29308, "stop": 30129, "strand": "+", "frame": 1, "gene": null, "product": "Lysine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "nt": "TTGAACCAAGATTTTGTTTTAAGAATAGCAACAGAAATTCCACTTAAAAAATTAGTTGTTGGTGGATTTGAAAGAGTTTACGAAATGGGTCGTATATTCAGAAATGAAGGATATGACACAACACATAACCCTGAATTTACAACTATTGAATTCTATGAAGCTTATTCAAATGTTGAAGGTATGATGAATAGAACTGAAACATTAATTAAAGAACTTTGCAAAAAAATTGGTAAAAGTAAATTTGTAACAAATGGTGTTGAAGTTGATTTATCAAAACCATTTAAACGTGTAAATATGATTGATGAAGTAAGCAAACAAACAGGTAAAAACTTTAGAAAAATTACTTTAGAAGAAGCTATTGAAGTAGCTAAAGCTTACAAAATTAAAATCGAAAAATTCTTTACCATTGGACACATTATTAATGCTTTATATGAAGAATTGGTTGAACCAACATTAATTCAACCTACATTCTTATATGGGCATCCAATTGAAATTTCTCCTTTAAGTGCTAAGAGCGATGATCCTAGATTTACTGAACGTGCTGAATTATTTATTAACACAAAAGAATATGCCAACATGTATACAGAATTAAGTGACCCAATTGATCAGTTAGAAAGATTTGAAAGTCAATTAGAAGAAAAGAATAAAGGTAATGATGAAGCAAGTGATATTGACTATGACTTTGTTGATGCTTTAGAATATGGCATGCCTCCAACAGGTGGTTGTGGAATTGGAATTGACCGTTTAGTTATGTTATTAACTGAAACAGATTCTATTAGAGATGTACTTTTATTTCCCACACTAAAAAGAATCAAAAAATAA", "aa": "MNQDFVLRIATEIPLKKLVVGGFERVYEMGRIFRNEGYDTTHNPEFTTIEFYEAYSNVEGMMNRTETLIKELCKKIGKSKFVTNGVEVDLSKPFKRVNMIDEVSKQTGKNFRKITLEEAIEVAKAYKIKIEKFFTIGHIINALYEELVEPTLIQPTFLYGHPIEISPLSAKSDDPRFTERAELFINTKEYANMYTELSDPIDQLERFESQLEEKNKGNDEASDIDYDFVDALEYGMPPTGGCGIGIDRLVMLLTETDSIRDVLLFPTLKRIKK", "aa_hexdigest": "5febca90a4d91fd2a0fa7446834a5834", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030049D44", "query_cov": 0.9926739926739927, "subject_cov": 0.8741935483870967, "identity": 0.664, "score": 362.0, "evalue": 3.68e-123, "valid": false, "gene": "lysS", "product": "lysine--tRNA ligase", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A1V5HK23", "kegg_orthology_id": "K04567", "go_ids": [ "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HK23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "product": "Lysine--tRNA ligase" }, "genes": [], "id": "IGIGPGHMMC_99", "locus": "GCA000148625_00059" }, { "type": "cds", "sequence": "contig_1", "start": 31469, "stop": 31801, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02126.24" ], "nt": "ATGAAGAAAGAAAAATTTGTTAGGACTGTGCTAGGCGATGTGCCTGCTAGTTCTATCGGTATTACTGACTGTCACGACCACCTTATAAAGAACGGTGGCCCTGAAATGGAAGAACACATTGACTTTTTAATGATCGATGTTGAAGCTGCTAAAAAAGAACTTCAAGAATTCGTTGATCACGGTGGAAAAACAATGGTTACAATGGATCCACCTAATGTTGGACGTGATGTATTTAGAATGATGGAAATTGCTAATGCATTCAAAGGCAAAGCAAACATTATTATGTCTACAGGTTTCCACAAAGCTAAATTCTATGACAAATACTGTTCATGA", "aa": "MKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCS", "aa_hexdigest": "14467491478e05d57b812bd0a5b99b68", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02126.24", "name": "PTE", "length": 92, "aa_cov": 0.8090909090909091, "hmm_cov": 0.9782608695652174, "evalue": 2.725283166638027e-07, "score": 26.286685943603516, "start": 17, "stop": 105 } ], "seq_stats": { "molecular_weight": 12380.227199999987, "isoelectric_point": 6.1835935592651365 }, "id": "IGIGPGHMMC_100", "locus": "GCA000148625_00060" }, { "type": "cds", "sequence": "contig_1", "start": 31835, "stop": 32530, "strand": "+", "frame": 2, "gene": null, "product": "phospho-furanose lactonase", "db_xrefs": [ "EC:3.1.1.25", "GO:0050490", "SO:0001217", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_C4XFK1" ], "nt": "ATGTGTGTCGCAGAAATTGAAGAAGGTATGGACGTTTACAACTATAATGGACCAGTTGTAAAACGTGGTAAAGCTAAAGCTGGTATTATTAAAGCTGGTACAGGTTATGCTGCTATTGACCGTCTTGAATTAAAAGCACTTGAAGTTGCTGCTAGAACCTCAATTACAACAGGTTGCCCAATTTTAGTACATACTCAATTAGGTACAATGGCTTTAGAAGTTGCACAACACTTAATTGACTTTGGTGCAAACCCTCGTAAAATTCAAATTTCTCACTTAAATAAAAACCCAGACCCATACTACTATGAAAAAGTTATTAAAGATACAGGCGTTACACTTTGTCTTGATGGACCAGACAGAGTTAAATACTACCCTGACTCAACTCTTGCAGATAACATTAAATACTTAGTAGACCAAGGTCTTCAAAAACATATAACATTAAGCCTTGATGCTGGTAGAATTCTATACCAACGTAACTATGGTTTAACAAAAGGTAAAGAAACATTTGGATTCTCATACTTATTTGATAGATTTATTCCTTTACTTAAAAAAGTTGGTGTTCCTCAAGATGCTATTGATGACATTTTGATCAACAACCCACGTGATATTCTTGCATTTGATGAACCTCGTAAATATGAACCATCAAAAGTATCAAACGAATTAAAACAACTTAAAAAAGATCTAAAACTAGATTAA", "aa": "MCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPRDILAFDEPRKYEPSKVSNELKQLKKDLKLD", "aa_hexdigest": "f3648094a87c89003d81640afa78d411", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 31535, "stop": 32530, "observations": { "mutation": [ 31799 ], "stop": [ 31799 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK1", "query_cov": 1.0, "subject_cov": 0.6434540389972145, "identity": 0.991, "score": 461.0, "evalue": 4.38e-162, "gene_start": 1, "gene_end": 231, "reference_start": 129, "reference_end": 359, "reference_sequence": "MQKRRFMKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCSWLANVPTDDIVKMCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPREILAFDEPRKYEPSKVSNELKQLKKNLKLD" }, "paralog": false, "identity": 0.9909365558912386, "score": 666.0, "evalue": 1.89e-245, "description": "Internal stop codon at 31799. Nonsense mutation around 31799.", "psc": { "uniref90_id": "UniRef90_C4XFK1", "product": "phospho-furanose lactonase", "ec_ids": [ "3.1.1.25" ], "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0050490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK1", "EC:3.1.1.25", "UniRef:UniRef50_Q4A724", "GO:0050490" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" } }, "id": "IGIGPGHMMC_101", "locus": "GCA000148625_00061" }, { "type": "cds", "sequence": "contig_1", "start": 32949, "stop": 33122, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTGTCTTAATGATGCAAATCGGTTCAGGAATGATTGTTGGTTTAGCTAAACCAGTGTTCCTTGCATTCTCTAAATTCGGTAAAGGGCTTACACCACTTGATACATACTTAGGACAAGTTTCAGTAGAAAACTTCCTTAACTCAGTTGGTCAAGGTTTTGCTTCATGA", "aa": "MIGVLMMQIGSGMIVGLAKPVFLAFSKFGKGLTPLDTYLGQVSVENFLNSVGQGFAS", "aa_hexdigest": "bf903f80e1dca179388b132ae892eb90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.034700000002, "isoelectric_point": 8.189692878723147 }, "id": "IGIGPGHMMC_102", "locus": "GCA000148625_00062" }, { "type": "cds", "sequence": "contig_1", "start": 33204, "stop": 33368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGGACACGTTATGTTCCAACAAGCAGCTGTTGTTGTTCCAGTTGTACTTGTATTAATGTTCTCAGGACAACAATTTAGAACAGCAGAAGGTGCTATTAATGTAGGCGGCCAAGTCGGTACAATCTTCTTCTCAGGTTTATTACTTGGTGCTTACTGA", "aa": "MITGHVMFQQAAVVVPVVLVLMFSGQQFRTAEGAINVGGQVGTIFFSGLLLGAY", "aa_hexdigest": "9438c055b1504e89d8b17de825ea8316", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5671.654299999998, "isoelectric_point": 6.50155086517334 }, "id": "IGIGPGHMMC_103", "locus": "GCA000148625_00063" }, { "type": "cds", "sequence": "contig_1", "start": 33447, "stop": 33689, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ], "nt": "ATGTTCGGTATTGCTATTGCATACCAAATTGGTAAATTCTTCGGTAAAAAAGAAGATTCAGCAGAAACTAAAAAACTTTCAAACAAAGTTAAATTATTAGAAGACAATATCTTCACACAAAGTGTATTGATCTTATTAATTTTCTTAATTCTTATCTTAATTATTCAATTCGCACCTGCAGGTGCATCAGTAAGATTTGGTTTAAGCAATGGACTTGTAAACAAAGCAATTTACGGTTCATGA", "aa": "MFGIAIAYQIGKFFGKKEDSAETKKLSNKVKLLEDNIFTQSVLILLIFLILILIIQFAPAGASVRFGLSNGLVNKAIYGS", "aa_hexdigest": "febae287c0e4d5a8ae6e901578a2b714", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B4D71", "query_cov": 0.8125, "subject_cov": 0.9848484848484849, "identity": 0.585, "score": 75.1, "evalue": 2.35e-16, "valid": false, "product": "PTS transporter subunit IIC", "uniref50_id": "UniRef50_A0AAX3F0H9" }, "pscc": { "uniref50_id": "UniRef50_A0AAX3F0H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8750.335599999991, "isoelectric_point": 9.631657218933103 }, "id": "IGIGPGHMMC_104", "locus": "GCA000148625_00064" }, { "type": "cds", "sequence": "contig_1", "start": 33768, "stop": 34391, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCTGGTATTCGTATGTTCGTTTCAGAACTTCAACAAGCTTTTCAAGGAATTAGTGAAAAATTAGTTCCAGGATCAGTTGTTGCTGTTGACGTTGCTGCCACATATGGTTTCAGCCAAAACTCAGTTACCTATGGTTTCTTATTTGGTTCAATTGGCCAATTCTTAGCTATGGGTCTTGTAATTGGATTAGCTCAAATTCCAAACTCAAGATTTGAAATTGTTATGGTAGTTCCATTATTCATTACATTGTTCTTTAACTCAGGTTCAATTGGAGTATTTGCAAATGCTTCAGGTGGATATAAAGCTACTATTGCAGTACCAATGATCTTTGGTTTTGTTGAAATCATTGTTGTTGCTCTTGCTTTAAGTATGATTAAAAACTTTGGTGTAATGCACCAAGTAGATCTTGACTTCCAATACTTTGAAAAAACTCTCACAAAAGCAGATGCATTCAAATCATTTACAGATTCATTAAATGCAAATTCTGCTAACTTGTTTATTCAAGGTGATGCTACTAAAGCTGTTTCAGCAGCACAATTAAATGATGCTATTACAAACATTAACAGTCAAGATTCATTCAAAATCTTAAGAAGCTTAAGTGAAGTTACAACAAACTGA", "aa": "MQAGIRMFVSELQQAFQGISEKLVPGSVVAVDVAATYGFSQNSVTYGFLFGSIGQFLAMGLVIGLAQIPNSRFEIVMVVPLFITLFFNSGSIGVFANASGGYKATIAVPMIFGFVEIIVVALALSMIKNFGVMHQVDLDFQYFEKTLTKADAFKSFTDSLNANSANLFIQGDATKAVSAAQLNDAITNINSQDSFKILRSLSEVTTN", "aa_hexdigest": "8e9c33c58f6d1edb99f60c3092190dc2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22227.381100000002, "isoelectric_point": 5.01293773651123 }, "id": "IGIGPGHMMC_105", "locus": "GCA000148625_00065" }, { "type": "cds", "sequence": "contig_1", "start": 34437, "stop": 34562, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATACTTATCAAGATATGTTTCAGACCAAATAGCTAAAGCTAAAGATATGAAAGAAATCTTGAAATTATCTTCATTCCAAAGAACACCATTCTTAACAGGATTTAATGGTATGTTTGACTGA", "aa": "MQYLSRYVSDQIAKAKDMKEILKLSSFQRTPFLTGFNGMFD", "aa_hexdigest": "8a8e49f17da4a40e9d73196cca569844", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4806.539099999999, "isoelectric_point": 9.301579093933103 }, "id": "IGIGPGHMMC_106", "locus": "GCA000148625_00066" }, { "type": "cds", "sequence": "contig_1", "start": 34647, "stop": 34820, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGCTGCTCAATTCTTTGACTCACACAGACAACACAAACCTACAGTATTCCAAAGAATGCTTAAAGTTAAAGTTGAAACTTTAGAAGATCAAAAGAAAGCTGAAGAAGTTGCTAATGCACCAGTTGAAGAAGTTCCTCAACCAGAAGTTGCAGAACAAGCTGCTTAA", "aa": "MIFAAQFFDSHRQHKPTVFQRMLKVKVETLEDQKKAEEVANAPVEEVPQPEVAEQAA", "aa_hexdigest": "9ef2f2516a6357660ae7dc9281ae3a0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6522.337500000003, "isoelectric_point": 5.218922233581542 }, "id": "IGIGPGHMMC_107", "locus": "GCA000148625_00067" }, { "type": "cds", "sequence": "contig_1", "start": 34919, "stop": 35197, "strand": "+", "frame": 2, "gene": null, "product": "PTS EIIB type-2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354302.1", "SO:0001217", "UniParc:UPI0001E32F02", "UniRef:UniRef100_UPI0001E32F02", "UniRef:UniRef50_A0A0D5ZIZ4", "UniRef:UniRef90_C4XFJ9" ], "nt": "ATGAAAGTATTATGTGTTTGTGGCTCAGGTATGGGCACAAGTATGATTATTAAAATGAAAGTTCAACAAGCTATGAAAGAACTTTCAATTCCAGGAACAGTTGAAGCTTTAGGTTTAGGTCAAGGTAAAACAGTTGTAAACAACTTTGATATTGTTTTATGTACCTCAAACTTTGTTTCAGAAGTATCAAAAGGCAAAGCAAAAGCTTATGGTTTAAAAAACATCATGGATGTTAATGAAATCAAAGCTGCTCTAAAAGATGCATCAAGCAAGATGTAA", "aa": "MKVLCVCGSGMGTSMIIKMKVQQAMKELSIPGTVEALGLGQGKTVVNNFDIVLCTSNFVSEVSKGKAKAYGLKNIMDVNEIKAALKDASSKM", "aa_hexdigest": "24690da3cdab7b54bc599a16779ceb06", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F02", "ncbi_nrp_id": "WP_013354302.1", "uniref100_id": "UniRef100_UPI0001E32F02", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F02", "RefSeq:WP_013354302.1", "UniRef:UniRef100_UPI0001E32F02" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F02", "uniref90_id": "UniRef90_C4XFJ9" }, "psc": { "uniref90_id": "UniRef90_C4XFJ9", "product": "PTS EIIB type-2 domain-containing protein", "uniref50_id": "UniRef50_A0A0D5ZIZ4" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZIZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZIZ4" ], "product": "PTS sucrose transporter subunit IIABC" }, "genes": [], "id": "IGIGPGHMMC_108", "locus": "GCA000148625_00068" }, { "type": "cds", "sequence": "contig_1", "start": 35407, "stop": 35685, "strand": "+", "frame": 1, "gene": null, "product": "PTS sugar transporter subunit IIA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "nt": "ATGCCACATGCTTCAGCAACAAAAGACGCTGTTTTTGGAAACGGATTCTCACTTATTACTCTTAAAAAACCTATCAAATTTGAAGGAGATGAAAGAGAAGTAAAAATCCTTATGGGACTAGCCGCAAAAGATGGTGAAACTCATACATCTGTTGCTATTCCACAAATTATTGCTGTTTTTGAAGATCCACAAAATATTGAAAAAATAGCACAAGCTAAAACAAAAGAAGAAATTATAAATATCATAAAAGGTGTTGATTATACTAAATACCTTAGTTAG", "aa": "MPHASATKDAVFGNGFSLITLKKPIKFEGDEREVKILMGLAAKDGETHTSVAIPQIIAVFEDPQNIEKIAQAKTKEEIINIIKGVDYTKYLS", "aa_hexdigest": "78542f5ce103e681644f869f4883fd5a", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFC0FB", "query_cov": 0.967391304347826, "subject_cov": 0.8557692307692307, "identity": 0.596, "score": 103.0, "evalue": 8.23e-27, "valid": false, "product": "PTS sugar transporter subunit IIA", "uniref50_id": "UniRef50_UPI002FEFC0FB" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEFC0FB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "product": "PTS sugar transporter subunit IIA" }, "genes": [], "id": "IGIGPGHMMC_109", "locus": "GCA000148625_00069" }, { "type": "cds", "sequence": "contig_1", "start": 35694, "stop": 36053, "strand": "+", "frame": 3, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGAAACCAATGTTACAAATTGCTTTAGATAACTTAACAATTGAAGATGCAATTGCAAGTGCCAAAAAGGTTGAAAAATACATAGACATTATTGAAGTAGGAACTATTCTTATTGCTTCAGAAGGTAAAAAAGCTATTAAAGCTTTGAGAGAAGCATTTCCTACAAAAATCATTGTTGCTGATGGCAAAATTGCAGATGCTGGGAAAGTTTTTGGAAAAATGTTTTTCGAAAATGGTGCTGACTTTACAACATGCATTTGTGCAGCTGAAGTGCCTACAATTGTTGAAACAATGAATATAGCCAAAGAATATGGCAAAAATAAAGAAGTTCAAATTGAATTAACTTCAAACTTTACTTGA", "aa": "MKPMLQIALDNLTIEDAIASAKKVEKYIDIIEVGTILIASEGKKAIKALREAFPTKIIVADGKIADAGKVFGKMFFENGADFTTCICAAEVPTIVETMNIAKEYGKNKEVQIELTSNFT", "aa_hexdigest": "c95f416a38be741e8cd606a348554817", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.865546218487395, "subject_cov": 1.0, "identity": 0.728, "score": 146.0, "evalue": 1.45e-43, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "IGIGPGHMMC_110", "locus": "GCA000148625_00070" }, { "type": "cds", "sequence": "contig_1", "start": 36328, "stop": 36492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAAAATAAAAAAAGACTTCTAGGCATCTATGAAAAAGCTATAAACAACAAATTTAGCTTAGAAGAAAAAATTATAATTGCTAAAGAAGCTGGTTATGACTTTATGGAATTTTCAGTTGATGAAAGTCCTCAAAGATTAGCTCGCTTAGACTGA", "aa": "MPNIENKKRLLGIYEKAINNKFSLEEKIIIAKEAGYDFMEFSVDESPQRLARLD", "aa_hexdigest": "89188bb3d15a1fee9f6ef502f4c23430", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.206900000001, "isoelectric_point": 5.368465614318848 }, "id": "IGIGPGHMMC_111", "locus": "GCA000148625_00071" }, { "type": "cds", "sequence": "contig_1", "start": 36556, "stop": 36756, "strand": "+", "frame": 1, "gene": null, "product": "Sugar phosphate isomerase/epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "nt": "ATGACCCTTAGTGGTCATCGTAAATATCCTTTTGGTTCTAAAGATCCTAAAATCAGAGCTAGAGCCAAAGAAATAATGACTAAAGCAATTATTTTAGCCAAAAAATTAGGTATTAGAACTGTTCAACTTGCTGGTTATGATGTTTATTATGAACCAGGTGATGAAGAAACAAGAAAAAACTTTATTGAAGGAATGAAATGA", "aa": "MTLSGHRKYPFGSKDPKIRARAKEIMTKAIILAKKLGIRTVQLAGYDVYYEPGDEETRKNFIEGMK", "aa_hexdigest": "2f1942fdec59377ad574e7a8d649bca4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A936AG02", "query_cov": 1.0, "subject_cov": 0.8048780487804879, "identity": 0.606, "score": 85.9, "evalue": 1.11e-20, "valid": false, "product": "Sugar phosphate isomerase/epimerase", "uniref50_id": "UniRef50_A0A936AG02" }, "pscc": { "uniref50_id": "UniRef50_A0A936AG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "product": "Sugar phosphate isomerase/epimerase" }, "genes": [], "id": "IGIGPGHMMC_112", "locus": "GCA000148625_00072" }, { "type": "cds", "sequence": "contig_1", "start": 36787, "stop": 36912, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTTGAAATTATGGATACTCAATTCATGGGAACTAATTCAAGAGCAATGACTTATATCAACATGATTAATAGTCCATATTTACAAATCTATCCTGATTTAGGTAATATTTATCAATGA", "aa": "MLAFEIMDTQFMGTNSRAMTYINMINSPYLQIYPDLGNIYQ", "aa_hexdigest": "78a88673ce127e65dc7852d2fbfdc0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.524100000001, "isoelectric_point": 4.0500284194946286 }, "id": "IGIGPGHMMC_113", "locus": "GCA000148625_00073" }, { "type": "cds", "sequence": "contig_1", "start": 36928, "stop": 37101, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGTATAACGAATTTGAACTTGCTAGAAATCATATAGTAGCTTTCCACTTTAAAGATACTGTACCTGGTAAATTTAGAGATACACCTTTTGGAACTGGCACTGTAGATTTCCCTTATCTTTTAAAAATATTAAAAGATTTAAAAATGAACCAACCTATTATGATAGAAATGTGA", "aa": "MYNEFELARNHIVAFHFKDTVPGKFRDTPFGTGTVDFPYLLKILKDLKMNQPIMIEM", "aa_hexdigest": "4ec6181250e55cf9a0c8a574a353f138", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 58, "aa_cov": 0.9473684210526315, "hmm_cov": 1.0, "evalue": 1.165957790500148e-10, "score": 37.551326751708984, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 6719.8261, "isoelectric_point": 6.707649040222168 }, "id": "IGIGPGHMMC_114", "locus": "GCA000148625_00074" }, { "type": "cds", "sequence": "contig_1", "start": 37206, "stop": 37310, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATAAAAAATATGAATCTGAAATCAAAAAACTTAAACAAGAAGTTTATGAAGCTAATATGTTATTAGTTAAATATGGACTTGTTATTCATACTTGA", "aa": "MAIDKKYESEIKKLKQEVYEANMLLVKYGLVIHT", "aa_hexdigest": "5dc66d56658d75c69e823906de281374", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4026.7597000000005, "isoelectric_point": 8.029940223693849 }, "id": "IGIGPGHMMC_115", "locus": "GCA000148625_00075" }, { "type": "cds", "sequence": "contig_1", "start": 37347, "stop": 37709, "strand": "+", "frame": 3, "gene": null, "product": "L-ribulose-5-phosphate 4-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "nt": "ATGGTTATTAAACCAAGTGGTGTTTCTTATGACACACTTAAAGCTGATGACATGGTCATAACTGATTTAGATAACAATGTATTTGATTCTAAATATCGCCCTTCTTCAGACACACCTACTCATACCTTACTTTATAAAGAAAATCCTGAAATAAAAGGTATTGTTCATACTCACAGTCCTCATGCAGTAGCTTTTGCACAAGCAGGTAAAGATATTCCTTGCTTTGGTACAACACATGCAGATAATTTTTATGGACCAGTGCCATGTGCTAGAGCTCTAACTCCAAAAGAAATTGATGGAGAATATGAACACAATACAGGACTTGTAATAATAGAAACATTTAAAAAACGCAAGATTGACTGA", "aa": "MVIKPSGVSYDTLKADDMVITDLDNNVFDSKYRPSSDTPTHTLLYKENPEIKGIVHTHSPHAVAFAQAGKDIPCFGTTHADNFYGPVPCARALTPKEIDGEYEHNTGLVIIETFKKRKID", "aa_hexdigest": "bb6b4f2a7f0b646380327b2d7ceac778", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6LVT1", "query_cov": 0.8416666666666667, "subject_cov": 0.9901960784313726, "identity": 0.594, "score": 128.0, "evalue": 2.97e-36, "valid": false, "product": "L-ribulose-5-phosphate 4-epimerase", "uniref50_id": "UniRef50_R6LVT1" }, "pscc": { "uniref50_id": "UniRef50_R6LVT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "product": "L-ribulose-5-phosphate 4-epimerase" }, "genes": [], "id": "IGIGPGHMMC_116", "locus": "GCA000148625_00076" }, { "type": "cds", "sequence": "contig_1", "start": 37818, "stop": 37937, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTAATACCTTAATAGTTTCAAATAATAGTGCTCCAACTGCGCAAGAAGCATTGATTGAAAAGCACTATAACCGTAAGCACGGAAAGAATGCTTATTATGGTCAAAATAAATAA", "aa": "MAINTLIVSNNSAPTAQEALIEKHYNRKHGKNAYYGQNK", "aa_hexdigest": "0f6872b9d7517e1b12f6900b7304aad9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4388.875100000002, "isoelectric_point": 9.60174388885498 }, "id": "IGIGPGHMMC_117", "locus": "GCA000148625_00077" }, { "type": "cds", "sequence": "contig_1", "start": 37912, "stop": 38298, "strand": "+", "frame": 1, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1", "UniRef:UniRef90_C4XFJ4" ], "nt": "ATGCTTATTATGGTCAAAATAAATAATATAGACTACTTCATTTTTGATTTAGATGGTACACTCTATGATGATAATAAAAACATCTTAGAATCAACCAAAAACACTTTAAAACAGCTTCAAAAGGAAAATAAAAATATAATAATTGCTACTGGTAGACCTTATTATATGAACTATAAATTAATTAAGGATTTAGATATTAAACTACCAATAATAGCAGTTAATGGGGCTATGATTTATGATCCTATCACTAAAGAAGTGCCTTATTTTCAAGGACTAGACAAAAAAGTTGTTCAAGAATTAATTAACTTTTTAGAAGAGAATAGAATTGATTTTTTAGCTTACGATATTAATGGTATGAAAGGTAATAATTTCTCGAACCCTTATTGA", "aa": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPY", "aa_hexdigest": "7c82ae5ddbbb9a93d9a8c8ed992b41db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 37912, "stop": 38601, "observations": { "mutation": [ 38296, 38347 ], "stop": [ 38296, 38347 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ4", "query_cov": 1.0, "subject_cov": 0.44912280701754387, "identity": 1.0, "score": 257.0, "evalue": 1.08e-84, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPYWFEFMIYKNVVETNPYRWDYQEGSLKETCNQFNFAKILIITQNVPQEKIDSLLTIASKFPKQIYTVSSQKGVIDIMPIGISKGETLKKIAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND" }, "paralog": false, "identity": 0.9868995633187773, "score": 448.0, "evalue": 2.38e-162, "description": "Internal stop codon at 38296, 38347. Nonsense mutation around 38296, 38347.", "psc": { "uniref90_id": "UniRef90_C4XFJ4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ4", "UniRef:UniRef50_A0A3P8MEN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1" ], "product": "Cof-like hydrolase" } }, "id": "IGIGPGHMMC_118", "locus": "GCA000148625_00078" }, { "type": "cds", "sequence": "contig_1", "start": 38521, "stop": 38769, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCCAATAGGAATATCAAAAGGTGAAACATTAAAAAAATTAGCTAAGATTAAAAATATTGATTTATCAAAAGCAATGGCTTTTGGTGATGCTGATAATGACATAAGTATGTTAAAATCTGTTAAAATTTCAGTTGCAATGGGTAATGCAACAGAGAATTTAAAGAAAGTTGCAACTTATGTAACAGATACAAATAATAATGATGGAATTGCCAAAATTATTAAAGAATTAGAGTTAGTAAATGACTAA", "aa": "MPIGISKGETLKKLAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND", "aa_hexdigest": "144f71b35865e30948b9073dc9fedc4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 74, "aa_cov": 0.9024390243902439, "hmm_cov": 1.0, "evalue": 1.459282633802837e-30, "score": 102.80609893798828, "start": 1, "stop": 74 } ], "seq_stats": { "molecular_weight": 8824.179999999997, "isoelectric_point": 7.9259527206420906 }, "id": "IGIGPGHMMC_119", "locus": "GCA000148625_00079" }, { "type": "cds", "sequence": "contig_1", "start": 38885, "stop": 39199, "strand": "+", "frame": 2, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3", "UniRef:UniRef50_UPI001F306D39", "UniRef:UniRef90_C4XFJ3" ], "nt": "ATGAGACATTTAGAAATTAAATATAATCCAGGGTATTTTAATTTCAATGGTGATCTAAGATTTAAAGCATTCAATGAGTTACAACGAGTACATAAAGTAGGTATCTCAATTAATCCTTCAGCGGTTAAAGATAAAGACAATTATTTCAAGTTTCATATTAGTGAAAATGTTGAAGTAGTGTTAGATGATTTATCACTAAAATTAATTAGTAAAGGTAGATCGTTTGCCTGTCCAATGCATTTCTTTGATGATTTATACTTAGAATATTTTGATGAGAAAAAAGTAACTAAAAATCAAAAATTGAGATTAACTTGA", "aa": "MRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKDKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLT", "aa_hexdigest": "42ee6cf8daede0351ca1ce8cbe4c8d1a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F306D39", "query_cov": 0.8269230769230769, "subject_cov": 0.8269230769230769, "identity": 0.7559999999999999, "score": 140.0, "evalue": 1.59e-41, "valid": false, "uniref50_id": "UniRef50_UPI001F306D39" }, "pscc": { "uniref50_id": "UniRef50_UPI001F306D39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F306D39" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 38762, "stop": 39250, "observations": { "mutation": [ 38810, 38849, 39197 ], "stop": [ 38810, 38849, 39197 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ3", "query_cov": 1.0, "subject_cov": 0.6419753086419753, "identity": 1.0, "score": 218.0, "evalue": 2.36e-71, "gene_start": 1, "gene_end": 104, "reference_start": 42, "reference_end": 145, "reference_sequence": "MTKIKVYKVNIIENRYWYCPSLFTFSRRLWASRPFATLEELMRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKDKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLTWRKYFFNIEVVEKEQTE" }, "paralog": false, "identity": 0.9814814814814815, "score": 320.0, "evalue": 5.58e-114, "description": "Internal stop codon at 38810, 38849, 39197. Nonsense mutation around 38810, 38849, 39197.", "psc": { "uniref90_id": "UniRef90_C4XFJ3", "product": "MPN499 family protein", "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ3", "UniRef:UniRef50_C4XFJ3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3" ] } }, "id": "IGIGPGHMMC_120", "locus": "GCA000148625_00080" }, { "type": "cds", "sequence": "contig_1", "start": 39362, "stop": 39766, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGATTAAATTAGGTGATGGTGTTTTTTATGGTTGTAGAAGTCTAACAAAAATTATCTTCAACAGTCAACTTCAAATAATTGGAAAAGAAACTTTTAGCCATTGTAATTCAATAGAAGAAATAAACATTCCAGATACTGTAAATTTAAACACTGAAGGTGCTTTTGAAATGTGTTTTAATTTAAAAACCGTTCATATCCCAAATAACACAACAATAATTAAAACTAATGCCTTTAAACAGTGCGAATCATTAGAAACATTAGACTTAACAAATACCAACGTGAATCAAATACAAGCAAATACATTTAAAGGATGTACAAACCTAAAAGAAGTTAAGGTCAAAAAAGACACTCAAATAGACCCTCAAGCAAGCGATAATAATTCAATTTTTGTTTTTAATATTTAA", "aa": "MIKLGDGVFYGCRSLTKIIFNSQLQIIGKETFSHCNSIEEINIPDTVNLNTEGAFEMCFNLKTVHIPNNTTIIKTNAFKQCESLETLDLTNTNVNQIQANTFKGCTNLKEVKVKKDTQIDPQASDNNSIFVFNI", "aa_hexdigest": "c764be6894559c8a073df3ec6f6d9980", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 125, "aa_cov": 0.9253731343283582, "hmm_cov": 0.968, "evalue": 3.110392332384639e-29, "score": 97.4892349243164, "start": 3, "stop": 126 }, { "id": "PF13855.12", "name": "LRR_8", "length": 51, "aa_cov": 0.373134328358209, "hmm_cov": 1.0, "evalue": 2.870343302386457e-13, "score": 45.46135711669922, "start": 61, "stop": 110 } ], "seq_stats": { "molecular_weight": 15036.963899999975, "isoelectric_point": 5.628674507141112 }, "id": "IGIGPGHMMC_121", "locus": "GCA000148625_00081" }, { "type": "cds", "sequence": "contig_1", "start": 40180, "stop": 40338, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTACAGTAGATGTTGAATACTTATCAAACCAAAATAAAGTAAGTGATGTTATCGTTGTAATTCTTAATGAATCAGAACACAAACACATTATTGATACAGCTCTTGGCGAAAAAATGACTAAACGTGCTGACAAATTAATGCTTGTTAAATAA", "aa": "MLCTVDVEYLSNQNKVSDVIVVILNESEHKHIIDTALGEKMTKRADKLMLVK", "aa_hexdigest": "0245acafaf42e63c52f2da9e2ed48dbf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5913.903799999999, "isoelectric_point": 6.024785423278809 }, "id": "IGIGPGHMMC_122", "locus": "GCA000148625_00082" }, { "type": "cds", "sequence": "contig_1", "start": 40672, "stop": 40863, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAACATTAGAAAAATTTTAGCTTCAGGTGAATTAGATGACATTGTTGTTGAAGCAAAAAGCGAACCAACACAACCAGTTGTAGTAGTTGAAGCTCCAACACAAGAACAACCTACTGTTCAAGAAACTCCAGCAGTTACACCTGAACCTCAAGTTGAAACATCATCAATGACAGAAACTAAATGA", "aa": "MNANIRKILASGELDDIVVEAKSEPTQPVVVVEAPTQEQPTVQETPAVTPEPQVETSSMTETK", "aa_hexdigest": "88e98fbed809d246d02c73f7a196e5b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6789.516500000002, "isoelectric_point": 4.16302433013916 }, "id": "IGIGPGHMMC_123", "locus": "GCA000148625_00083" }, { "type": "cds", "sequence": "contig_1", "start": 40912, "stop": 41178, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGGCAACCCCAGGTCGTGGTGAAACAGTTAGATTATTCACCAAAAAAGACTTCCAAGCTAAACAAGATGGAAATGATGAAAATTCTTCAAGTAAAGATACTTCAGCAAGAAACGCAAGAGAATATGCAATGATTCTTGCTTTAGGCGGTCCAAAAAACATCGTTAACACAAATAACTGTGCTTCAAGATTAAGATATGATATTAACGATAGAAACTTAGTAAATGAAGTTGAATGTAAGGCAGCAGGTGCAGTTGCACTTAAATGA", "aa": "MATPGRGETVRLFTKKDFQAKQDGNDENSSSKDTSARNAREYAMILALGGPKNIVNTNNCASRLRYDINDRNLVNEVECKAAGAVALK", "aa_hexdigest": "85e4901081aca55cc3e0ad07257329e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 31, "aa_cov": 0.3522727272727273, "hmm_cov": 1.0, "evalue": 1.750571817855501e-12, "score": 42.57704544067383, "start": 45, "stop": 75 } ], "seq_stats": { "molecular_weight": 9604.646399999994, "isoelectric_point": 9.061563301086426 }, "id": "IGIGPGHMMC_124", "locus": "GCA000148625_00084" }, { "type": "cds", "sequence": "contig_1", "start": 41404, "stop": 41586, "strand": "-", "frame": 2, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607", "UniRef:UniRef90_UPI002FF38607" ], "nt": "ATGCAAGGCAAATACTCATTCATGATTCTAGGATTACCGGGTGCAGCTGTTGCTATGATTTTAGCGGCTCCTAAAGAAAATAGAAAAGTGGCAGCTGGTACAGTTATTCCTGCAGCTCTTACTTCAGTTATTACTGGTGTTACAGAACCTATTGAATTTACATTCTTATTCTTAGCACCATGA", "aa": "MQGKYSFMILGLPGAAVAMILAAPKENRKVAAGTVIPAALTSVITGVTEPIEFTFLFLAP", "aa_hexdigest": "a333f9de734758970e8316a67670feb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 41356, "stop": 41604, "observations": { "mutation": [ 41406 ], "stop": [ 41406 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FF38607", "query_cov": 0.9833333333333333, "subject_cov": 0.7108433734939759, "identity": 0.831, "score": 98.2, "evalue": 1.18e-25, "gene_start": 2, "gene_end": 60, "reference_start": 8, "reference_end": 66, "reference_sequence": "MKIGRFADGKFSFMIFGLPAAAAAMILAAPKENRKLALGTVVPAAFTSIITGVTEPIEFTFLFLAPLLFWGFHALICIFIYVC" }, "paralog": false, "identity": 0.7439024390243902, "score": 130.0, "evalue": 4.76e-41, "description": "Internal stop codon at 41406. Nonsense mutation around 41406.", "psc": { "uniref90_id": "UniRef90_UPI002FF38607", "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FF38607", "UniRef:UniRef50_UPI002FF38607" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607" ], "product": "PTS transporter subunit EIIC" } }, "id": "IGIGPGHMMC_125", "locus": "GCA000148625_00085" }, { "type": "cds", "sequence": "contig_1", "start": 42013, "stop": 42252, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGGTAAATTTGGTGCTGAAGTTAAACAAGTCGTTGGTTCTTCATTAGGATTCAAAACTCTTCAAACTTCAGTTTTCGGTGGTATTTTTGTTGGTTTAATAGTTTCATACTTATACAACAAATTACACACAATTAAACTTCCAACTGTTATTTCATTCTTTGGAGGAAAACGTTTTGTTTCATTAATAACAATAGTTGCTATGATTCCATTAGCATTCTTATTCCTCCTTATATGA", "aa": "MTGKFGAEVKQVVGSSLGFKTLQTSVFGGIFVGLIVSYLYNKLHTIKLPTVISFFGGKRFVSLITIVAMIPLAFLFLLI", "aa_hexdigest": "6fb69cb74b120360696917bdbe8af925", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8585.338099999994, "isoelectric_point": 10.218255043029785 }, "id": "IGIGPGHMMC_126", "locus": "GCA000148625_00086" }, { "type": "cds", "sequence": "contig_1", "start": 42253, "stop": 42780, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTACAGCACAAACTACAGGTATAGAAAACAAAAAATCTAAACCAAAAGACAATAGCCGTAAGGGAAATTTTCGTAAGATTCTAAGTAAAATCTCTGGTGCTTTCATGTTACCAATTTCCGTTATGTCTATAGCTGGTCTATTTCTAGGGGTTGGTGCAACAATATCAAGTCAATCAGCGATTGTTCTTAACAACAATCCATTATCATCTGCTGCTAAAGCTGGTATTGTAATTGGTGACTTTATTAAGTTATTAGGTGACCCAATATTTGGAGCAATGCCGCTATTGTTTGCCGCTGCCTTTGTTGTGGCATTTACCGACGAAGCCGGAGTCGGTGTCTTCGCCGCAATAGTTGGATACTTAGTATTCTCAGCAATACAAGCTCCATTTATTCAAAGTGTTATAGAAAAAACTAATATTGATGGCACTAAACAAGCTAGTGAATTAATAGGTAAAACAATACTTAAAGATGATAAAACAGGACAACTTTACTATGAAACATTAAAAGGATATAACATTCTTTGA", "aa": "MTTAQTTGIENKKSKPKDNSRKGNFRKILSKISGAFMLPISVMSIAGLFLGVGATISSQSAIVLNNNPLSSAAKAGIVIGDFIKLLGDPIFGAMPLLFAAAFVVAFTDEAGVGVFAAIVGYLVFSAIQAPFIQSVIEKTNIDGTKQASELIGKTILKDDKTGQLYYETLKGYNIL", "aa_hexdigest": "a215c937b1056fca8d3d7aade24d8e93", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18467.3631, "isoelectric_point": 9.42574520111084 }, "id": "IGIGPGHMMC_127", "locus": "GCA000148625_00087" }, { "type": "cds", "sequence": "contig_1", "start": 43463, "stop": 44167, "strand": "+", "frame": 2, "gene": null, "product": "BPL/LPL catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "nt": "ATGAAAATTTTTAGAATTAAGGAAACTTCACCTTATGTTACTTTAGCTTTAGAAAATATTATTATGCATGATCATGATTTAAAAGGTGATGTACTTTTAATTTATCAACATGATAATGCTATTATTATTGGTAACAATCAAAATGCATATGAAGAAATTAACCGTTCTTTTGTAAAAGAAAATAATATTAAATTAGCTCGTAGAATGTCTGGCGGTGGAGCTGTGTATCATGATTTAGGCAATATTAATTTTAGTTTCATAACTGACTATGATAAAAAAGGCGGTTATGAAAGATTTCTAACTCCAATTATTGCTTTTCTTAAAAGCTTAGGTCTAGATGCTCAATTTAAAGGTCGCAATGATGTTGTGGTTAATGGCTGCAAAATAAGTGGTAACGCTCAATATATTAGTAATAATCGAATAGTAAGCCATGGAACATTATTATTTGATGTTGATTTAACAAAATTATCGAAAGCACTTAATCCTGCAAGAATTAAGTATGAATCTAAAGGCATTCAATCAGTTAGATCTAGAGTAGTTAATATTTATGATGAATTATTAAACAAAATGACAGCTGAAGAATTTATTAGTCGTCTAATTAATTATTTTGTTAAGAATTTTAAAGGCGAATTTATTGATATTGATTATGCAAAATATCAAGAACAATTAAACTCTTTACAACAAAAATTTGCTTCTGAAGAATGA", "aa": "MKIFRIKETSPYVTLALENIIMHDHDLKGDVLLIYQHDNAIIIGNNQNAYEEINRSFVKENNIKLARRMSGGGAVYHDLGNINFSFITDYDKKGGYERFLTPIIAFLKSLGLDAQFKGRNDVVVNGCKISGNAQYISNNRIVSHGTLLFDVDLTKLSKALNPARIKYESKGIQSVRSRVVNIYDELLNKMTAEEFISRLINYFVKNFKGEFIDIDYAKYQEQLNSLQQKFASEE", "aa_hexdigest": "15eb3472949c422187a5f6ed7da5e547", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00111975EB", "query_cov": 0.9658119658119658, "subject_cov": 0.9576271186440678, "identity": 0.6729999999999999, "score": 311.0, "evalue": 7.48e-105, "valid": false, "product": "lipoate--protein ligase", "uniref50_id": "UniRef50_A0A432FB30" }, "pscc": { "uniref50_id": "UniRef50_A0A432FB30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "product": "BPL/LPL catalytic domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_128", "locus": "GCA000148625_00088" }, { "type": "cds", "sequence": "contig_1", "start": 44456, "stop": 44944, "strand": "+", "frame": 2, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "ATGTATAAAAGACAATCAATTGAACTTTTAGGCGAAACAATTTATTATGAAAGAATAGATTGTTTTCAAAAAGAAAAAAGACCAGCAACTGTTTTACTTGTGCACGGTTTTAATTCTGATTCAAATACCTTTACTAGATTTATTGATTTTGCTGAAGTAGTGGCCGAAAATACACCCGATAATGACGAATTAAGAGAAGAATATAGAAACTTTCATCGTTTCAATTTATATGCTTTAGATTTGCCTGGTTGTGGAAAATCTGGAAAAAATAGTCAAATAACACTTGATTTATATAACAAAATAGTAGTTGAATTTATTAACACAGTTTTAAAAGACCACGAAGTAATTTTAATTTCGCATTCAATGGGTTCAGTACCTTGTTTGTATGCTAAAAAAGAATGCAAAAACGTAACTAAGTTATTTATGGTAGCTCCTTTAAATTATGCTTTAGTTGACGATAAGCAAAAACTAATTAATCTTAAAAAATGA", "aa": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTKLFMVAPLNYALVDDKQKLINLKK", "aa_hexdigest": "e1716fda80ef0091d6c175668e074e62", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 44456, "stop": 45247, "observations": { "mutation": [ 44942 ], "stop": [ 44942 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.552901023890785, "identity": 0.9940000000000001, "score": 329.0, "evalue": 2.1e-112, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.9923954372623575, "score": 531.0, "evalue": 2.21e-194, "description": "Internal stop codon at 44942. Nonsense mutation around 44942.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "IGIGPGHMMC_129", "locus": "GCA000148625_00089" }, { "type": "cds", "sequence": "contig_1", "start": 44945, "stop": 45307, "strand": "+", "frame": 2, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "TTGATTCCTGCTAATGTTGAAGATGCTAGAAATTCATTAAAAGCATTGGTTTATAATCCTGAAGATTATTATTTAAGAAACTTGGATTATAATGCTGAAAAATTAGCGAGCAAATATAATGAAAACAAAGAAACTTTTTCTTATCTATTAAATGAACAAATTTTAAATAAAGATTATTTATTAAAACATGTAAAGCCTTTATTTGCAAATCCAAATGGTTGTGTAATTATTTATGGTGAAAATGATGAATTTGTGACACCAAAAGAAATTAAAAAAATAGAAGAAGATTATGGTTATAAAATTGTGCAATTAAATGAATGCGGACATGCTGCATTTTATCAAAAATTAAATACCATTTATTGA", "aa": "MIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIY", "aa_hexdigest": "30b36ee128c2e7586cf6ca2c83cdd545", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 44645, "stop": 45337, "observations": { "mutation": [ 44942, 45305 ], "stop": [ 44942, 45305 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.40955631399317405, "identity": 0.992, "score": 248.0, "evalue": 4.41e-81, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.9869565217391304, "score": 461.0, "evalue": 1.55e-167, "description": "Internal stop codon at 44942, 45305. Nonsense mutation around 44942, 45305.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "IGIGPGHMMC_130", "locus": "GCA000148625_00090" }, { "type": "cds", "sequence": "contig_1", "start": 45487, "stop": 45981, "strand": "+", "frame": 1, "gene": null, "product": "Glycosyltransferase 2-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6", "UniRef:UniRef90_C4XFI6" ], "nt": "ATGAATAGGCAAAAAACTTTATATCAACCTAAAATTTCAATTTTAATTCCCTTGCATAATAGTGCTAAATATTTTAATTCCACTATTAAAACTATTCTTAATCAAAGTTATGAAAATTTAGAAATTTTATTCCATGATGATGCCTCTAATGATGAAACAAGAAAAATCTTAGAAGATTTGACCATTAAAGATTCTAGAGTTAAATATATCTTTTCTGATACTAATATTGGTTCAGGATATTCAAGAAACAAATTAATTGAAGCTTCTACCGGAGATTTCATTTTTTTCATCGATGATGATGATGAATTTTTAAGTCCTAAAATAATAGAACGTTGTGTTAAATATTTAAAGCCTAATTTAGAAATACTTTCAACACAATTTAAATATAAATTTGATGATTTAGGTATTAAAATACCTATCAATAATATTGTTAAAACATTGAAAATCAATCCTTCACCAATGCATTATTATTTGAATACCACAACTTTTGCTTGA", "aa": "MNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFA", "aa_hexdigest": "819d4df325371f604d7a18fecc4fcf32", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "both", "pseudogene": { "start": 45415, "stop": 46284, "observations": { "mutation": [ 45439, 45979 ], "stop": [ 45439, 45979 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI6", "query_cov": 1.0, "subject_cov": 0.4568245125348189, "identity": 1.0, "score": 327.0, "evalue": 1.19e-110, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MFIFIDNYWMFSKISNIYYIKRKNMNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFAWGHFFNKKFLYDIDVNFSKVKKYEDIAEMGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDVSFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKIPKVKK" }, "paralog": false, "identity": 0.986159169550173, "score": 574.0, "evalue": 3.38e-210, "description": "Internal stop codon at 45439, 45979. Nonsense mutation around 45439, 45979.", "psc": { "uniref90_id": "UniRef90_C4XFI6", "product": "Glycosyltransferase 2-like domain-containing protein", "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI6", "UniRef:UniRef50_C4XFI6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6" ], "product": "Glycosyltransferase 2-like domain-containing protein" } }, "id": "IGIGPGHMMC_131", "locus": "GCA000148625_00091" }, { "type": "cds", "sequence": "contig_1", "start": 46063, "stop": 46494, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAACATTTTTTCCAAATGTAAGAATTTTAAAAGAACTTTGATTTATTCTATAATTTATCATAGAAAAACCAATTCATTAACTTCATTTGATAAAAACTATATCAATAAAATTGAATGTATCAACCAAGCCTACAAATTTAATTTAGAAAACATAGTATCAAATTTAAAAAATGATATAAGTTTTAAAATTCTTAAAAAAACTGTAAATTCCTTATTTATGGAACATTTAAGTTTGATTAGTGTTTACTATATTAATTTAAAAAATCGAAATTTAAAAAAAGAATTTAAAAAATTCATCAAAGATATTTATCCTTCAATTTTAGAAATATATAATGGTTTTGATGTAAAAATCAAATATCGAAGAATTTCTGATTTACCAGTTTTTGTTACTAAATTTTTATATAAAAAACCAAAAGTTAAAAAATAG", "aa": "MGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDISFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKKPKVKK", "aa_hexdigest": "0011f1245bccb0ab8b2a0570ffbbfe99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17199.225199999993, "isoelectric_point": 9.982236289978026 }, "id": "IGIGPGHMMC_132", "locus": "GCA000148625_00092" }, { "type": "cds", "sequence": "contig_1", "start": 46507, "stop": 47499, "strand": "+", "frame": 1, "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "db_xrefs": [ "EC:5.1.3.2", "GO:0003978", "GO:0006012", "RefSeq:WP_013354314.1", "SO:0001217", "UniParc:UPI0001E32F0E", "UniRef:UniRef100_UPI0001E32F0E", "UniRef:UniRef50_A0A507SK18", "UniRef:UniRef90_C4XFI5" ], "nt": "ATGACTTATTTATTAATTGGTGGAGCAGGTTATATCGGAAGCCATGTAGCTGAAGTTATAAATAAAGAAAATCAAAATAAAGTAATTATTTATGATAATCTTTCAACAGGATTTAAAGAATTTATTGAAGAAAAAAGTAAATTCATTCATGGAGATATTTTAGATATAAAAACATTAGATTTAACTTTTAAAAAAAATAAAATAGATGTAGTAATTTATTTAGCAGGTTTAATTAAAGTTGGTGAAAGTGTACAAAAACCACTTGACTATTATGAAACAAATATTCAAGGCTTAGTAAATGTTTTAAAAACAATGAAGAAGTATAATGTTAATAATTTGGTATTTTCTTCATCAGCAGCAGTATATGGTAATAACTCATTACACGATGGTTATTTTTTTGAAGAAGACCCAAAAGAACCATGTAGTCCTTATGGTAGAACCAAATATTTTGGAGAACAAATTATCAAAGACTTTGCTAATACAAATAGTGATTTCAAATATACTTTTTTAAGATACTTCAATGTTGCCGGTGCTTCAAAAAGTAAAAGAATTGGTTATTTAACCAAAAATGGTGTTGCACCGACTCATCTTATACCTGCTATTAGTTATTATGCTTTTGGTTTAATTCCCGAATTCAAAATTTTTGGTAATGACTATAACACACCTGATGGGACATGCATAAGAGATTATGTATATGTATATGAATTAGCAGAATTACATTTATTAACTGCAAAAAAAATGATTAAAGAAAATACTTCATTATATTACAACATTGGTAGCTCAAAAGGCTTTTCAAATCTTGAAGTTGTAAATGCTTTTGAAAAAAATTTAAGCAAGAAATTAAATATTGAATATGGTCTAAGAAGAGCAGGAGACCCTGATAAATTAATAGCTTCAAATAAAAAAATATGTAAAGAATTAAATTATGAAGTAAAAACTAATTTAGATGAAATAATTTCTAGTGAGATTGAATTTAGAAAAGAACATTTATAA", "aa": "MTYLLIGGAGYIGSHVAEVINKENQNKVIIYDNLSTGFKEFIEEKSKFIHGDILDIKTLDLTFKKNKIDVVIYLAGLIKVGESVQKPLDYYETNIQGLVNVLKTMKKYNVNNLVFSSSAAVYGNNSLHDGYFFEEDPKEPCSPYGRTKYFGEQIIKDFANTNSDFKYTFLRYFNVAGASKSKRIGYLTKNGVAPTHLIPAISYYAFGLIPEFKIFGNDYNTPDGTCIRDYVYVYELAELHLLTAKKMIKENTSLYYNIGSSKGFSNLEVVNAFEKNLSKKLNIEYGLRRAGDPDKLIASNKKICKELNYEVKTNLDEIISSEIEFRKEHL", "aa_hexdigest": "ca8372d824175dc554f136a3347dbe55", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F0E", "ncbi_nrp_id": "WP_013354314.1", "uniref100_id": "UniRef100_UPI0001E32F0E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F0E", "RefSeq:WP_013354314.1", "UniRef:UniRef100_UPI0001E32F0E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F0E", "uniref90_id": "UniRef90_C4XFI5" }, "psc": { "uniref90_id": "UniRef90_C4XFI5", "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "ec_ids": [ "5.1.3.2" ], "uniref50_id": "UniRef50_A0A507SK18", "go_ids": [ "GO:0003978", "GO:0006012" ] }, "pscc": { "uniref50_id": "UniRef50_A0A507SK18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A507SK18" ], "product": "UDP-glucose 4-epimerase" }, "genes": [ "galE" ], "id": "IGIGPGHMMC_133", "locus": "GCA000148625_00093" }, { "type": "cds", "sequence": "contig_1", "start": 47538, "stop": 48014, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4", "UniRef:UniRef90_C4XFI4" ], "nt": "GTGAATCTCACTTTTTTTGTTTTGTCCTTGTATAATATAAATTGCATCAAAAGAATTAAAAATAAAAATAATGAATTTATAACTGAATATATACATGTTTTTGTCGAAAAATACGATCTTTTAACATTTATGAATTCGTTAAATAGAAATAAATTACATCCCAATAATGTAAGAATTGAAACAGTTATTAAAGCACTAGAAATGCTTAAAGAATTAAATAACTTTAAGAATATTCATAAATCTTTAAATTTAAAACGCGAAACATTTCAAAAACTATTTGATGACTTATCAGATGATAGAAAAATGATTTCCAAAACCGATAAATTTTTTAAAGGTTTGACGTGCCCACATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTTCAGTTTTAAATAAATACAGAAATTGTAGAAGAATAATAGTTAATCAAAAAATTAAAAATAAACACAAAGATTGA", "aa": "MNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMLKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKD", "aa_hexdigest": "4fdb62d96da015879b563475b9c0576a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 47496, "stop": 48317, "observations": { "mutation": [ 48012 ], "stop": [ 48012 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI4", "query_cov": 1.0, "subject_cov": 0.4906832298136646, "identity": 0.987, "score": 311.0, "evalue": 9.38e-105, "gene_start": 1, "gene_end": 158, "reference_start": 15, "reference_end": 172, "reference_sequence": "MKCVKVIKARPTILVNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMFKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKDWIKYINQNSNVYDRLCNENRKNSLRTFCQKSVRTLIDEAKEKCLKIKFPHFTTIYKNINILIFDINISILPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLMNRATRNFICDYANEMQIQLKKR" }, "paralog": false, "identity": 0.9853479853479854, "score": 544.0, "evalue": 3.64e-199, "description": "Internal stop codon at 48012. Nonsense mutation around 48012.", "psc": { "uniref90_id": "UniRef90_C4XFI4", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI4", "UniRef:UniRef50_C4XFI4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4" ], "product": "Transposase" } }, "id": "IGIGPGHMMC_134", "locus": "GCA000148625_00094" }, { "type": "cds", "sequence": "contig_1", "start": 48219, "stop": 48464, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAAGTCTGTAGGAAAGGGAGAAAGGCATAAAAAAGACCCTTCAATTCCAAAGAAAAGAAGAGTCGGATTACATATAAGTTTAAGACCAGAAATTACAACAATGTTCGATTTTGAATTTGATACAGTACATGGCAAAAGCGATGATTTATATCATATTGCTGTTTTAATAAATAGAGCAACACGAAATTTTATATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAA", "aa": "MPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLINRATRNFICDYANEMQIQLKKR", "aa_hexdigest": "0ca13fbe60b74ce2007dcef2fd3f9eac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9481.884499999993, "isoelectric_point": 9.774712562561035 }, "id": "IGIGPGHMMC_135", "locus": "GCA000148625_00095" }, { "type": "cds", "sequence": "contig_1", "start": 48419, "stop": 48679, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3", "UniRef:UniRef90_C4XFI3" ], "nt": "ATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAATACATATGATTGATGAACATGAATTAAATATTAATACTTTAACCATTGACAATGGTTCAGAAAATTACACTTTAGATCAGATCAACACTATTAAGCAAATATATCATTGCGATGCTTTCAATTCAAGTCAAAAAGGTAGAATTGAAAATTGTAATAAAATGATAAGAAAATATAGACCAAAATCAATTACTTTTGATAATTTAACACATGAATGA", "aa": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIENCNKMIRKYRPKSITFDNLTHE", "aa_hexdigest": "5972fdd74903af043be0378b27a8c95f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 48419, "stop": 48826, "observations": { "mutation": [ 48677 ], "stop": [ 48677 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI3", "query_cov": 1.0, "subject_cov": 0.6370370370370371, "identity": 0.988, "score": 175.0, "evalue": 5.46e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIKNCNKMIRKYRPKSITFDNLTHEWIEIINEKINNFVRHEVDDFPFAMSANQFEKFIQENKHLYPSFYTNLYA" }, "paralog": false, "identity": 0.9851851851851852, "score": 276.0, "evalue": 2.46e-97, "description": "Internal stop codon at 48677. Nonsense mutation around 48677.", "psc": { "uniref90_id": "UniRef90_C4XFI3", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI3", "UniRef:UniRef50_C4XFI3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3" ], "product": "Transposase" } }, "id": "IGIGPGHMMC_136", "locus": "GCA000148625_00096" }, { "type": "cds", "sequence": "contig_1", "start": 49343, "stop": 49906, "strand": "+", "frame": 2, "gene": null, "product": "Glycosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2", "UniRef:UniRef90_C4XFI2" ], "nt": "GTGAAATTTAAGACTCAAACTCAAAAGCTAAAATATGGTATAGTCATTTGTGCTAGAAATGAAGAAAAAGTTATTGCTGAGTTAGTAAAAAGCATAAAAAATTCTCAATATGATCAAGATAAACTCCAAACATTTGTGATAGCACATAATTGTACAGATAAAACAGCGGAAAAAGCAAGAAATGCTGGTGCTATTGTTTATGAATATTCTAATTCTAGCGAAAGAACTAAGGGTTATGCATTAAAAAAAATATTTGAATTCATAGAACAAGACTATGGTATACAAAGTTTTGATGGATATCATATATTTGATGCGGACAATATAGTTGATAGTCTATATTTTGAAAAAATGAATGATGCTTTTTTACACTATGATAAAAAAAATGTAATTACTTCATTTAGAAATACTAAAAATTTTGGTAAAAGCATTCAAACAATTAATTATGGACTTATGTTTATGTTCCATACAACCATTGAATCAAACGCTAGAATGAAGTTTAAGATGTCTTCAAAAATTTTGGGTTCTGGTTTTTTAATTAGCAATGAAATGGTTAAAGATGGTTGA", "aa": "MKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDG", "aa_hexdigest": "bd160d29b4c432524c9c95793c6169ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 49226, "stop": 50209, "observations": { "mutation": [ 49307, 49904, 50030, 50048, 50072 ], "stop": [ 49307, 49904, 50030, 50048, 50072 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI2", "query_cov": 1.0, "subject_cov": 0.43488372093023253, "identity": 0.995, "score": 367.0, "evalue": 7.11e-125, "gene_start": 1, "gene_end": 187, "reference_start": 40, "reference_end": 226, "reference_sequence": "MVMVYQNVVYIVMPIIVAIFLIFSFQYWFVLFFGLFAKPVKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDGWNITIPSDDSDFTVEQISEGKNVKYCDEAMFYDEQPTKFKDMWRQRLRWVFGNRIVWRKRFKSLIKNIFSSKKEDDKKISKSSSIDILFMISPISFIGFLILLLNILLVSLGPIFGANATQMWIDWAISFSISHAIIYLILFVIGIITYARGRKRVQRVPWWKMIISLFIWPLFLALYFPLQIVAIFKNINKFNWTQIEHNKNI" }, "paralog": false, "identity": 0.981651376146789, "score": 628.0, "evalue": 3.86e-230, "description": "Internal stop codon at 49307, 49904, 50030, 50048, 50072. Nonsense mutation around 49307, 49904, 50030, 50048, 50072.", "psc": { "uniref90_id": "UniRef90_C4XFI2", "product": "Glycosyltransferase", "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI2", "UniRef:UniRef50_C4XFI2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2" ], "product": "Glycosyltransferase" } }, "id": "IGIGPGHMMC_137", "locus": "GCA000148625_00097" }, { "type": "cds", "sequence": "contig_1", "start": 50550, "stop": 50861, "strand": "+", "frame": 3, "gene": "cofD", "product": "2-phospho-L-lactate transferase CofD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1", "UniRef:UniRef90_C4XFI1" ], "nt": "ATGAAAATAACTATTATTTCAGGTGGATCAGGTAATGATGCACTAGTAAAAGGATTAAAAGCTTTTTACAAAGATAAAAATTTAAGCATTAAAGTTATTGTAAATGCTTATGATAATGGAAAATCTACTGGTGTATGTAGAAAAGTTACTGATACTTTAGGAGTATCAGATATAAGAAAAAATCACTCAAGAATGTATGTGAGTGAATATGGCGATAAAGTGGATAAAAACCTTTTAGAGTTTTATGAAAAAAGATTTGATTTCACTAAAAATAAAGAAGAATTAGAAATTTCTAAACTTTTAGATAAATGA", "aa": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDK", "aa_hexdigest": "04bbf442d71f3436bf8ce0bed53fd935", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "cofD" ], "truncated": "3-prime", "pseudogene": { "start": 50550, "stop": 51045, "observations": { "insertion": [ 51006 ], "mutation": [ 50859 ], "stop": [ 50859 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI1", "query_cov": 1.0, "subject_cov": 0.6280487804878049, "identity": 1.0, "score": 202.0, "evalue": 3.01e-65, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDKWNLKEYKKYVSNFFKNPKANEFNYKDFSVSNIVYSQMYKELGYEKTNKHFAIKWELMISLF" }, "paralog": false, "identity": 0.9939024390243902, "score": 323.0, "evalue": 4.96e-115, "description": "Internal stop codon at 50859. Frameshift due to insertion around 51006. Nonsense mutation around 50859.", "psc": { "uniref90_id": "UniRef90_C4XFI1", "product": "2-phospho-L-lactate transferase CofD", "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI1", "UniRef:UniRef50_C4XFI1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1" ] } }, "id": "IGIGPGHMMC_138", "locus": "GCA000148625_00098" }, { "type": "cds", "sequence": "contig_1", "start": 50967, "stop": 51125, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAGAATTAGGTTATGAAAAAACAAATAAACATTTTTGCGATAAAATGGGAATTGATGATTTCGTTGTTTTAAATTCATTTGATAATATCTTTATCAAGGCATTAAAGGAATCTGGAACAATAATAGATGATGAAGGAAAAACTGTCTTTTGA", "aa": "MYKELGYEKTNKHFCDKMGIDDFVVLNSFDNIFIKALKESGTIIDDEGKTVF", "aa_hexdigest": "22472f0ddd429be777e9cfe594aa1272", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6008.802399999999, "isoelectric_point": 4.832019233703612 }, "id": "IGIGPGHMMC_139", "locus": "GCA000148625_00099" }, { "type": "cds", "sequence": "contig_1", "start": 51390, "stop": 51572, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAACCGGTCTTGACTTATCAAATGTAAAAATTTTACTTAATAAAAGTGCAAAAGAAACCTTAAAATTAACCATACCAAACTATAATTTCATAGTCAAAGATATGGGAAATATTAAGGGTAAACATGAACCAGAAAAAATGGTTAAATCTGTTATTGATACCTATTATGATAAATAA", "aa": "MERTGLDLSNVKILLNKSAKETLKLTIPNYNFIVKDMGNIKGKHEPEKMVKSVIDTYYDK", "aa_hexdigest": "9528d9b5f1ca56da7fc1f06b823dfa37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6914.030600000004, "isoelectric_point": 9.26070613861084 }, "id": "IGIGPGHMMC_140", "locus": "GCA000148625_00100" }, { "type": "cds", "sequence": "contig_1", "start": 51585, "stop": 51881, "strand": "+", "frame": 3, "gene": null, "product": "MobA-like NTP transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9", "UniRef:UniRef90_C4XFH9" ], "nt": "ATGAAATTCGGAATTATACTATCTGCTGGTAATCAAACTAGATTTAAAAGCGCAACTCCTAAAGCCTTGGTTAATGTAAATGGAAAAATTTTACTTGAGGAAAATATTTTGCGAATGTCAAAATATTGTGATCATATTTTTACGATATGTTCTTCCCAAAATGAGCATTTTTTTGAAAAATATAATCATATTGCTATCTCATCAGGTAAGGGTAGTGGAGATGCTGTACTAAAAGCATTATTGGAAATCAAACAAAAATATGGTTTTTCTTCAAATGATACTTGTTTTATATTATGA", "aa": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFIL", "aa_hexdigest": "e22e22e646e501c11daa6d2383647b4f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 51585, "stop": 52184, "observations": { "mutation": [ 51879 ], "stop": [ 51879 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH9", "query_cov": 1.0, "subject_cov": 0.4224137931034483, "identity": 1.0, "score": 199.0, "evalue": 7.13e-63, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFILWGDCLIEDLIYKKVVENYKKVGIVPCVFEEKPYVQIFQKQDNKIDVLFSKFGENASSGFHDLSIFYFNCEEILKYLLDFQNKIIGRDGNYVHKHGNEMEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK" }, "paralog": false, "identity": 0.9949748743718593, "score": 404.0, "evalue": 3.55e-146, "description": "Internal stop codon at 51879. Nonsense mutation around 51879.", "psc": { "uniref90_id": "UniRef90_C4XFH9", "product": "MobA-like NTP transferase domain-containing protein", "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH9", "UniRef:UniRef50_C4XFH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9" ], "product": "MobA-like NTP transferase domain-containing protein" } }, "id": "IGIGPGHMMC_141", "locus": "GCA000148625_00101" }, { "type": "cds", "sequence": "contig_1", "start": 52170, "stop": 52283, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTTTAGATGTTTTTAATGAAACAGATATAAAAGCTCAAATATTAGAAATCAAAAATTATCAAGATATGTCATTTAACACTATAGAACAATTAAAAAATATAAAATAG", "aa": "MEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK", "aa_hexdigest": "416431f6fb9cd22a558de94e679412d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4465.0621, "isoelectric_point": 4.440682792663575 }, "id": "IGIGPGHMMC_142", "locus": "GCA000148625_00102" }, { "type": "cds", "sequence": "contig_1", "start": 52590, "stop": 52835, "strand": "-", "frame": 3, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011B1B315" ], "nt": "TTGATTAAATATAAACTAAAAATTTTTATATATAAAGCTGGTCCATATGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCATCCAAGAGAAATTTTTGGATATTTATTTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGA", "aa": "MIKYKLKIFIYKAGPYASFQRGSNENFNGLVRRFFKKKTNFDDITEEEILKVQNEINNHPREIFGYLFALEMYEKLNAENP", "aa_hexdigest": "455307731160c72d5cf6ebbddd1af5d9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011B1B315", "query_cov": 0.9876543209876543, "subject_cov": 0.8602150537634409, "identity": 0.563, "score": 92.4, "evalue": 7.12e-23, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI0011B1B315" }, "pscc": { "uniref50_id": "UniRef50_UPI0011B1B315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011B1B315" ], "product": "IS30 family transposase" }, "genes": [], "id": "IGIGPGHMMC_143", "locus": "GCA000148625_00103" }, { "type": "cds", "sequence": "contig_1", "start": 53747, "stop": 54493, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE52", "UniRef:UniRef90_C4XE52" ], "nt": "ATGTTTTGGTTTCTTATTGAAAGATTTTTATATATAATTACATTTATGATAGAAATAGTTAATTTATCAAAAAAAGATAAAAAAAATCGATTGATTTTAAACAACTTAAATTTCAATATTCATGAAAATGAATTGCATGTTTTTTTGGGCAAAAATGGTGCTGGAAAAACAGTTACTTTAGGAATAATTTGTGGATTAGATTCAGAATTTTCTGGAAAAATTTTAAAAGATAATGTAGATATTTACAAATCAAATAAAACTAATAATTATTTGTTTGTTCCAACAGATATTAGATTTCCAAATCATTTAAAAGTTATTGATTTAATTAAATTTTATGTGAATGAAATTTTTGAATTAGAAACAAATGAATTATTTATAAATGAAACATTAAAAGAATTCCATTTAGATGAAAGAAAGGACTATCTTCCTAAAAAATTATCTTCTGGAGAAAAAAGAAAATTAATGCTATTAATATCTAAATTAGTAAAACTAAAATATTTATTCTTAGATGAACCAGAAGCTAATCTAGATCCTTATTCAAGAAATGAATTATTTAAACTTTTGAATATGTTAAAAAAAGAAGGAACTTCAATTTTCACATCATCTCACTTAATATCTGAAGAATTGCAAGATATAGATAGAATAACAATTTTGCATAACAAAACTATCGTTCTAAGTGAAAAAGTAAAATCAGCAAAAAATTTAAAAATTATTTTTGATAATTTTAAGAGAGATGGTTATGACTAA", "aa": "MFWFLIERFLYIITFMIEIVNLSKKDKKNRLILNNLNFNIHENELHVFLGKNGAGKTVTLGIICGLDSEFSGKILKDNVDIYKSNKTNNYLFVPTDIRFPNHLKVIDLIKFYVNEIFELETNELFINETLKEFHLDERKDYLPKKLSSGEKRKLMLLISKLVKLKYLFLDEPEANLDPYSRNELFKLLNMLKKEGTSIFTSSHLISEELQDIDRITILHNKTIVLSEKVKSAKNLKIIFDNFKRDGYD", "aa_hexdigest": "a216b756b1bd8e6d0ebff3a24e9723f9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XE52", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9840000000000001, "score": 472.0, "evalue": 6.14e-168, "valid": true, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE52" }, "pscc": { "uniref50_id": "UniRef50_C4XE52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE52" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_144", "locus": "GCA000148625_00104" }, { "type": "cds", "sequence": "contig_1", "start": 54633, "stop": 55460, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51", "UniRef:UniRef90_C4XE51" ], "nt": "ATGATTTTTCTATTGGATTTTATTATAGTTTGTGTGTCAACATTATTTATTCTGCATTTAACATTTGTCTCAAACCAAAAATCGATATATGAAAACACATTAAATTGTAGATATTTTAATAAACCTAAAATTGTTTTAGCTTGGTCAATATATTCATTTATTTTATTATCCGCATTTTCATTATTAAAGTCTTTTGCAATAAGTTCATTGCATTTATATAGAGACACGAATATAAAAAGTTTTCTACTTTATTTTATAGGCTATTTTCTAAGTAATTTATTGATAATTATTTTTATTATTTTAGTTTTTGCAAGTTTTATATTTATAACAAAATCATATAAAAAGTCTCTCGTCATTGCAACTTGTATATTACCTTTTTTTACTGTTGTGTCTTTATCATCAAAAACAATAGAAGCGCGAGCAAATAAAAAGGTAGAAATAAACAAGGATATAATAGTTTCTGATGACAAGTATTACTTTTTATCAGATGATGAGATTAAAAGTAAATATAATTTGAACTTAATTTCATCTATATATTTTTTACCCAAAAATACTATAAATAAGTACTTTGATGAGAATTTCTTGACTTATAAAAATAATTCAGATACAAAAAATTATATATTCTCAACATTCAATTTGTCTATTCTAAAAAATAATAAAAAATTTGACAAAGATAAATTTAGAATTTATCATAACGATGGTGAGTTTTTTATAAAAATGAGTACAAATGAATTGGCTAATTATTTATATAGCAAATTAAAAAAATATTTTGAATCAGGTGGAGTTAATTTAGATAAATTACTTAAAAAAATAAAATCTAATATATGA", "aa": "MIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSDDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNI", "aa_hexdigest": "54ed4f5fa57825de10277d35de54a141", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 54486, "stop": 55745, "observations": { "mutation": [ 54537, 54630, 55458, 55551, 55566 ], "stop": [ 54537, 54630, 55458, 55551, 55566 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE51", "query_cov": 1.0, "subject_cov": 0.48672566371681414, "identity": 0.996, "score": 508.0, "evalue": 6.22e-177, "gene_start": 1, "gene_end": 275, "reference_start": 50, "reference_end": 324, "reference_sequence": "MTKKILSLTFKFNKKNKWVYIGLSLSFIISFIFCSLFLFDNYLFYNYFWMIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSGDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNIWIKNNFTGNEYNLVMHLLGKDDFNFQYLQRDWNDNLWNAAKLKSNLNSMFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSNSKITDSDLNFISEKMVNFVGDKVIIDFNKYAQSELENSITTTITKFNLYFPEIDSKEKWKNLVYSSSNHLFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE" }, "paralog": false, "identity": 0.9809069212410502, "score": 808.0, "evalue": 1.04e-297, "description": "Internal stop codon at 54537, 54630, 55458, 55551, 55566. Nonsense mutation around 54537, 54630, 55458, 55551, 55566.", "psc": { "uniref90_id": "UniRef90_C4XE51", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE51", "UniRef:UniRef50_C4XE51" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51" ], "product": "ABC transporter permease" } }, "id": "IGIGPGHMMC_145", "locus": "GCA000148625_00105" }, { "type": "cds", "sequence": "contig_1", "start": 55602, "stop": 55745, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAATTAATTTCTTTGATTTTTTAAATCATTTATACAGATCAAATCAAACAAAATTTAACACCACAATTAACATGGCTGAATTTTATATTGTTAAAGATAATAATTTAATAAAATACTTAGATTCAAAAGAAAAATGA", "aa": "MFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSKEK", "aa_hexdigest": "59e021d0cdcde63613b1a50658faf01c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5798.602100000001, "isoelectric_point": 9.10056667327881 }, "id": "IGIGPGHMMC_146", "locus": "GCA000148625_00106" }, { "type": "cds", "sequence": "contig_1", "start": 55776, "stop": 56015, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCATTATTAAACAAACAATTGATAAAATGAATGAACTTGTATCTAATTTAGGAAATTATCAAGCGTTTAAATTTGAAGAAAACGTAAATCCCAAAAACTTTTATGACAATACAAAAATAATAAAACTAAATAATTTGACATATGACCTGATAAACTTATTTTTAATAACATTTGTTATTTTAACACTCAGCATTTTGCCAATAATATTTACTTTTAAAAGGAGAAAACATGAATAA", "aa": "MFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE", "aa_hexdigest": "093021895d27c9b3ac1f070b46c997bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9458.04919999999, "isoelectric_point": 9.346964836120605 }, "id": "IGIGPGHMMC_147", "locus": "GCA000148625_00107" }, { "type": "cds", "sequence": "contig_1", "start": 56008, "stop": 56970, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAGAAATTAAAAATTAATTTACTATCTATTTCAACAATAATGATGGGGGCATCATTTGTTGTCGCATCATCTTGTGGTTCTAAAGAACAAGAGCAAGAAAAAGTTTTAAGTGATAATGAATATGTTGAGAAACACTCTATAACAAGAGACACAAATATTAAGGAAACAAGATATATTAGTCAAAATGTAAACAAAGTTTTTGACAACCACATACAATTAGAAAATTTTGTTAAAAATAATATAAAATCTGTTGATTTGAAATTTTTAAAAAACTCAAAAAAATTTGATGATATAAATAAAAATAATCCACAATTTATGTCCAATGAAGAGCTTTCATATTTTGATTTTAGCAAGTCAACAAGTAACGAAAAAATGCCGATATTTTTAGGACGAAACGGGAAAGTTTTTACTTCATTAGAAAAAGCAAGAGATTCATATTTTAATATAAATCAAGTTTACAAATTAGGCAATATTTATGCAAACAGTAAAGAGGAACTTTACAATTTCATTTTAAATAAATCAGTAAATAATAAAGATTTTTTGATAGAAGTATTAAATGAAATAAAAAGCAAATTGAATTTTAATTCACCTAACGCAACTATTACAGAATTTTTCAAAAGTCCAAGCGGACAAATTACAAGTTTTAAAAAAGAAGACCTTAAAGATGCAGACAAAAAAAGCAGAATTGAAGATTACATTAAAAATGTTGCAAAGAAATATGTAATCTATTATGATGAAGAAAATAATCCTCACTTAAAAGCGGTTGAAGATTATATATTTGATAGAATATCAAACTCTGAATTTATTAAGAGTAAAATCACTAGAATTAATTCTAATAAAGGATATAACAAATATTATGTAGACAATTCCAAAGATGATGAATTTACTTTGTATGGGCCAAGAATTTTAGAGACTCCTTTTGAAAATATTGAAGAAATAACTGATACAACTAAATGA", "aa": "MNKKLKINLLSISTIMMGASFVVASSCGSKEQEQEKVLSDNEYVEKHSITRDTNIKETRYISQNVNKVFDNHIQLENFVKNNIKSVDLKFLKNSKKFDDINKNNPQFMSNEELSYFDFSKSTSNEKMPIFLGRNGKVFTSLEKARDSYFNINQVYKLGNIYANSKEELYNFILNKSVNNKDFLIEVLNEIKSKLNFNSPNATITEFFKSPSGQITSFKKEDLKDADKKSRIEDYIKNVAKKYVIYYDEENNPHLKAVEDYIFDRISNSEFIKSKITRINSNKGYNKYYVDNSKDDEFTLYGPRILETPFENIEEITDTTK", "aa_hexdigest": "6697426d5bd46219397135d13fa442ae", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37353.61180000001, "isoelectric_point": 8.22224941253662 }, "id": "IGIGPGHMMC_148", "locus": "GCA000148625_00108" }, { "type": "cds", "sequence": "contig_1", "start": 57028, "stop": 57468, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAGTATTGTCACATCACTTTTCAAAATTGTGTATTTTAATGAAAACATAAAGAAAAATGTTGAAATTACACCATTTACACCTATAACAATAAATGTTGAAAAAGATCATTTAAACAATTTCAAAAAATGTACCAAAAACTTTTGTTTTGAAGGCACTAACCTATACGATTATTTATATAACAAATATGATCTTTTAGCAAAAGGAAAAGAATCTAATTTTCTTTCTAGTTTTTATATATTATATGTTGCGGGTTTAAACAAAATTATAGAATATAAAGCAAATCAAAAAGTGGTTGATGAATATAAAACATATTTTAAAATGATCATAAATGACATTAGCAAAATTTTTGCATCATTATTACCAAAATATTTCGTTCATGAAGGAATTAGTGAATCAGAAAACTCAGATAATCAAGTAAATATATCAAAAATTTGA", "aa": "MNSIVTSLFKIVYFNENIKKNVEITPFTPITINVEKDHLNNFKKCTKNFCFEGTNLYDYLYNKYDLLAKGKESNFLSSFYILYVAGLNKIIEYKANQKVVDEYKTYFKMIINDISKIFASLLPKYFVHEGISESENSDNQVNISKI", "aa_hexdigest": "63d72098871a81715a0f67735920f3b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17076.431299999997, "isoelectric_point": 8.372783088684084 }, "id": "IGIGPGHMMC_149", "locus": "GCA000148625_00109" }, { "type": "cds", "sequence": "contig_1", "start": 57514, "stop": 57777, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCTAAATTATTGAAATATAAAATAGCATGCAATTTTTTGGATGTAATTGCAAGTGGCATATTTAATGCAGTATATAGATTTTCAAGTGAAGATTATGATCCAGAATCTCTCATTTTTCCTGAATATGCAATATATGATAATTATGAACATTATTTTGAAATTTATAGTTTAAAGCGATTTAAAAAACCAGTTTTTTCAAAAAATTATGACAAATTTACTAAACAATCATATGATACTAACAACAGCTTACAAGAATGA", "aa": "MIAKLLKYKIACNFLDVIASGIFNAVYRFSSEDYDPESLIFPEYAIYDNYEHYFEIYSLKRFKKPVFSKNYDKFTKQSYDTNNSLQE", "aa_hexdigest": "58bb8d6021ab725a8297f91dfee73712", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10441.674799999988, "isoelectric_point": 5.790495109558106 }, "id": "IGIGPGHMMC_150", "locus": "GCA000148625_00110" }, { "type": "cds", "sequence": "contig_1", "start": 57793, "stop": 57891, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGACAGTAAAAAACAACTTTGATAATGTGAATGAATTAGTAAAAGATATTCTTCTAAATAATGAAGATTTATTAAACAAAGATAAAAGTTTTTGA", "aa": "MSTVKNNFDNVNELVKDILLNNEDLLNKDKSF", "aa_hexdigest": "16e0d69176099dc4f8a134ae5fe80175", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3725.1410000000005, "isoelectric_point": 4.528214836120605 }, "id": "IGIGPGHMMC_151", "locus": "GCA000148625_00111" }, { "type": "cds", "sequence": "contig_1", "start": 57988, "stop": 58359, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATTTGTTGCCAGTAGAAAAAAAATCATTTGCTAAATATTTATTATATGCTTTCATAGAAATAGATAATATAGTTAAAAAAGGTAGTTGGTCAAGCAAAGTTAATCTTTTAGTAGAAACTACAAAATATATTCATTCAATAATAGATGCAAAAGCAGCAGCATTAGAAATAGCTTTAAATGTTGTCGGAGGTCCAGTTTCTGCTTTTGGTGGTTTATTGTCTAACTTTTATGTTAAATTTTACAAAGCATTATTTGGTGAATTTAGCCAATTTAACTATAAATACGTTGTCAATGCAGGAGATAAAGGTAATGTCGATTATATATGGAATGGTGGATTGCGTTATGTTGGTTTATTTCATTGA", "aa": "MKKDLLPVEKKSFAKYLLYAFIEIDNIVKKGSWSSKVNLLVETTKYIHSIIDAKAAALEIALNVVGGPVSAFGGLLSNFYVKFYKALFGEFSQFNYKYVVNAGDKGNVDYIWNGGLRYVGLFH", "aa_hexdigest": "c9ad9b5a9f148db23e3d8e95c12b079b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13798.869099999989, "isoelectric_point": 9.323756217956543 }, "id": "IGIGPGHMMC_152", "locus": "GCA000148625_00112" }, { "type": "cds", "sequence": "contig_1", "start": 58414, "stop": 58590, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGGTTATACTGAAAGTTTTTATTACTTCAATGGTAAAAAATATAACTTTGTTGATATAGATTTATTAAAAAGAGATGTTGTAAATTACTATTTAAAAGGAAACAACAAAATTATAAACAACTTATTAGAAAAAGATGAAGCTGAAAAGAACATAAAAATTATTTACTCATGA", "aa": "MDGYTESFYYFNGKKYNFVDIDLLKRDVVNYYLKGNNKIINNLLEKDEAEKNIKIIYS", "aa_hexdigest": "5f30dddf43e072cd30bfbcd985bdd02e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6978.839899999999, "isoelectric_point": 6.139145469665528 }, "id": "IGIGPGHMMC_153", "locus": "GCA000148625_00113" }, { "type": "cds", "sequence": "contig_1", "start": 58831, "stop": 59337, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTATTTATTATTCAAAAAAACCAATAGAAACAGAAGATGGTTACACCGACAAGCTATTTCCTATTGATCAATTTGATCCAACAAAAAAAGCTTTTGAAGACAAAAAAATACTAGCAAATATTTATAATGAAGATCCATTAAAAAATTGCTTTTTATATTCAGAAATAGGAAACTTTAATATTCAAAGCGATTTGTTAAAAAATAATAGAAACAAATTTATTCCATTTTTAAATTCATTGAATATAAGAACAAAAGAAGTTTCAAATATATCATTTTTTAATAATTCAAATTATCAAGATATGGAATTGTTGCAAACAAAAACACTTTATTATTTTGATTATAAAAACAATAAATACTTCTTTACAAATTTAGACAAAGCGAGAAATTATTTTATCAATGTTATTATTAACCCAAAAATGGAATTATATAATTTAACCAAAAGAACCAAAATTATATTTATGAATAAAGAGTTCTGTACATTGAATGAAGCAGTATCTTGA", "aa": "MNPIYYSKKPIETEDGYTDKLFPIDQFDPTKKAFEDKKILANIYNEDPLKNCFLYSEIGNFNIQSDLLKNNRNKFIPFLNSLNIRTKEVSNISFFNNSNYQDMELLQTKTLYYFDYKNNKYFFTNLDKARNYFINVIINPKMELYNLTKRTKIIFMNKEFCTLNEAVS", "aa_hexdigest": "7e129a86dc9ae178808e7d802a2d0e76", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.779200000008, "isoelectric_point": 8.669337654113772 }, "id": "IGIGPGHMMC_154", "locus": "GCA000148625_00114" }, { "type": "cds", "sequence": "contig_1", "start": 59321, "stop": 60043, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E45A26", "UniRef:UniRef90_UPI0002E45A26" ], "nt": "ATGAAGCAGTATCTTGATACAAAAAGAATAATAAAAGAGGCGAAAATAAAAATTAAAAAATTATTTTTATCTTTAATTCCAACAGTTGTTTTGATTCCATCATGCAATAATTCAGCCTCTATGAATTATTATATGCAAAACAATAATTTAAAAAACGCAAGCAAATATGAAATTAATTTAGAAGGACAAAAATACCAATATACTGATATTGAATCATTTATGTCTAATTTAATAAATAGTAATAAAATAAAAGTTGAAAACAAATTAGTAATAGGCAAAAAGAAAAACAATAGTTCAAATAAAATTCTAGATTCTACTATTTTTTATAATTTCGACCCAAATAAACTTAAAAAAGTTTATCAAAATAAATTCCAAAAAACGGTTTATTCAGTTGATGAAGTAATAGAATCGTTTAGAGAAGAAAGCTCATATATTAAAAAATATCTTTTTGCCAATAAAGAATTTGATTCAGAAGAAAAAGTAAAACAGTATATAAAAACTCAACTTAAAAACGGTTTTAATAAATATGTTTATTATTCTAATAATGGGAAAAAATATAATGCTATGAATTGGCAAGATTCATTAGAATTGCTAAAAGATAAATATAATAAAGATAACAATGTGTTTTATCCTATTTTTGATAACAACAATAATGTAATTTATAATAATTTACTTAATTATGAAACAATGAATTCTATTAGTAAAAAATTCTTGAAAAGTTAA", "aa": "MKQYLDTKRIIKEAKIKIKKLFLSLIPTVVLIPSCNNSASMNYYMQNNNLKNASKYEINLEGQKYQYTDIESFMSNLINSNKIKVENKLVIGKKKNNSSNKILDSTIFYNFDPNKLKKVYQNKFQKTVYSVDEVIESFREESSYIKKYLFANKEFDSEEKVKQYIKTQLKNGFNKYVYYSNNGKKYNAMNWQDSLELLKDKYNKDNNVFYPIFDNNNNVIYNNLLNYETMNSISKKFLKS", "aa_hexdigest": "67b000d118a41a1a6c15b70a6223343e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002E45A26", "query_cov": 0.8166666666666667, "subject_cov": 1.0, "identity": 1.0, "score": 377.0, "evalue": 2.14e-131, "valid": true, "uniref50_id": "UniRef50_UPI0002E45A26" }, "pscc": { "uniref50_id": "UniRef50_UPI0002E45A26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E45A26" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28524.30580000004, "isoelectric_point": 9.512132835388183 }, "id": "IGIGPGHMMC_155", "locus": "GCA000148625_00115" }, { "type": "cds", "sequence": "contig_1", "start": 60043, "stop": 60153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTTAAAGTAGAAATAAAATTTGGTTTAGATCTCAATGATGAAAAGTCTTATGACTACAATGAAAAGGATTTAGGTTTTAAAGTAAATGTAAAAAATAATAAATGA", "aa": "MKFKVEIKFGLDLNDEKSYDYNEKDLGFKVNVKNNK", "aa_hexdigest": "3fc8f501b02a59d211ef3b7c02b2b1b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4311.865400000003, "isoelectric_point": 7.983780860900879 }, "id": "IGIGPGHMMC_156", "locus": "GCA000148625_00116" }, { "type": "cds", "sequence": "contig_1", "start": 61045, "stop": 61386, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTCAAAGAAAAACATGTATAGAATTTTTAAACAAAATAATTTCTTCTAAATCATTTCCAAGTGCTTCTGAACCCGAAGACAAACAAAAACTTTTAAAAGAAATTATTGAAGAACATAAGAATAAAAACAAATTAAATAATAGCTTTTTTGAAAATAATTTGCCCTTACTTATTAATGATAAAAACATTGAATTATTTTCTTTCTATTCAAATAATAATGTTGACATCTTATCAAAAAGATATTCAATACCTTCTAACATACTACTTGAGTTTCCAACTTTTTATTCTAAAGATGATTATGAAAATAACATAGCTCTTTATTTTGACGAAAATTGA", "aa": "MIFQRKTCIEFLNKIISSKSFPSASEPEDKQKLLKEIIEEHKNKNKLNNSFFENNLPLLINDKNIELFSFYSNNNVDILSKRYSIPSNILLEFPTFYSKDDYENNIALYFDEN", "aa_hexdigest": "847c1d7cacac38fcc8b6662a8cebaf44", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13409.995499999979, "isoelectric_point": 5.182545280456542 }, "id": "IGIGPGHMMC_157", "locus": "GCA000148625_00117" }, { "type": "cds", "sequence": "contig_1", "start": 61471, "stop": 61674, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_259637299.1", "SO:0001217", "UniParc:UPI0021806470", "UniRef:UniRef100_UPI0021806470", "UniRef:UniRef50_UPI0021806470", "UniRef:UniRef90_UPI0021806470" ], "nt": "TTGAGATATTTACTTAGTAATCAATTAGGGTATAAAGAAGATTTAGTTGCATATGATAATAAAGGTGTAGTAGAAATAATTAACAATGACATTTTCAATGTGTTTGTATTGAAATTAGAAGGCAATGAGTTTTCATTCCTGAGTTATAATGATGCAATTTCATTTTTAAAAGATTACATAATAACAAGATCAAAATTAATATAA", "aa": "MRYLLSNQLGYKEDLVAYDNKGVVEIINNDIFNVFVLKLEGNEFSFLSYNDAISFLKDYIITRSKLI", "aa_hexdigest": "5157b607e73f61726553e1640d9390ce", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0021806470", "ncbi_nrp_id": "WP_259637299.1", "uniref100_id": "UniRef100_UPI0021806470", "db_xrefs": [ "SO:0001217", "UniParc:UPI0021806470", "RefSeq:WP_259637299.1", "UniRef:UniRef100_UPI0021806470" ] }, "ips": { "uniref100_id": "UniRef100_UPI0021806470", "uniref90_id": "UniRef90_UPI0021806470" }, "psc": { "uniref90_id": "UniRef90_UPI0021806470", "uniref50_id": "UniRef50_UPI0021806470" }, "pscc": { "uniref50_id": "UniRef50_UPI0021806470", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806470" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7828.896299999995, "isoelectric_point": 4.810363578796387 }, "id": "IGIGPGHMMC_158", "locus": "GCA000148625_00118" }, { "type": "cds", "sequence": "contig_1", "start": 62022, "stop": 62351, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "ATGAGTGATATTAATGCTAAATTAGTTTATGCAATTATCACACACATTAAAAACGAAGTTAGAGCTAGAGGCTTAAAAGATACTAAAGATATAGGTGAATTAGTTGCTGATCAAATGTTTGTTGTTTATACAAACAAAAGTATTGTTGATACAACTTTAAATTATCAAGATGGAAGATTAAATATCTTTATTTTTGTTGGAGTTAATGGTAGTGGTAAAACAACTTCAATTGCTAAAATAGCCTATAAATATATAAAAGAAGGAAAAAAAGTTTTAATTGCTGCGGGCGATACTTTTAGAGCTGGCGCAGTAGACCAATTAGGTGTGTGA", "aa": "MSDINAKLVYAIITHIKNEVRARGLKDTKDIGELVADQMFVVYTNKSIVDTTLNYQDGRLNIFIFVGVNGSGKTTSIAKIAYKYIKEGKKVLIAAGDTFRAGAVDQLGV", "aa_hexdigest": "54fa3717e7fe33575a41645ca6c556b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.44036697247706424, "hmm_cov": 1.0, "evalue": 7.441250306837859e-20, "score": 67.41764831542969, "start": 61, "stop": 108 } ], "seq_stats": { "molecular_weight": 11912.65809999999, "isoelectric_point": 9.362372779846194 }, "id": "IGIGPGHMMC_159", "locus": "GCA000148625_00119" }, { "type": "cds", "sequence": "contig_1", "start": 62505, "stop": 62828, "strand": "+", "frame": 3, "gene": "ftsY", "product": "Cell division protein ftsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "nt": "ATGAAAGAACTTGAAAAAATGTATGGTATTATTGCTAGATTCCAAGACAAAGCGCCACACGAATGTTTATTAGTTTTAGATGCAACAACTGGTCAAAATGGAGTTAGCCAAGCTAGAAGTTTTAAAGAAGTTGCTGCCCCAACGGGAATTATATTAACCAAAATGGATGGAACTTCTAAAGGTGGAATTGTACTTTCAATTAAAGACGAATTTGATTTGAATGTTAAATATATTGGACTTGGCGAAGGGCTAGATGATCTTCAAGAATTTGATTTAGATAACTTCATTTATGAAATGACAAAGGACTTAATTGTAAATGAATAA", "aa": "MKELEKMYGIIARFQDKAPHECLLVLDATTGQNGVSQARSFKEVAAPTGIILTKMDGTSKGGIVLSIKDEFDLNVKYIGLGEGLDDLQEFDLDNFIYEMTKDLIVNE", "aa_hexdigest": "9c8e085c190240b900b91241932ea558", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PPJ9", "query_cov": 0.9719626168224299, "subject_cov": 0.9203539823008849, "identity": 0.76, "score": 157.0, "evalue": 9.43e-48, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_E4QS11" }, "pscc": { "uniref50_id": "UniRef50_E4QS11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "product": "Cell division protein ftsY" }, "genes": [ "ftsY" ], "id": "IGIGPGHMMC_160", "locus": "GCA000148625_00120" }, { "type": "cds", "sequence": "contig_1", "start": 62821, "stop": 63051, "strand": "+", "frame": 1, "gene": null, "product": "Sigma-70, region 4", "db_xrefs": [ "KEGG:K09787", "RefSeq:WP_013354325.1", "SO:0001217", "UniParc:UPI0001A8C3BB", "UniRef:UniRef100_C4XE46", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_C4XE46" ], "nt": "ATGAATAAAGATATTAAAGATATAGAAAATAGAGAGCACTTATTAGAACTATTTGATAAATTTAATTCATTTTTAACTCAAACACAAAAACAAGCTTTTCAATTATATTTCATTGAAGATCTTTCTTATTCTGAAGTTGCCAACATTACAGCAACCACCCGTTCTGGAGCTTATGATAGTGTGAATAAAGCAATTAAAAAATTACTAGATATTGAAGCAAAAATGCAATAA", "aa": "MNKDIKDIENREHLLELFDKFNSFLTQTQKQAFQLYFIEDLSYSEVANITATTRSGAYDSVNKAIKKLLDIEAKMQ", "aa_hexdigest": "ca972cf6120e9f43c803240e82563c66", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C3BB", "ncbi_nrp_id": "WP_013354325.1", "uniref100_id": "UniRef100_C4XE46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3BB", "RefSeq:WP_013354325.1", "UniRef:UniRef100_C4XE46" ] }, "ips": { "uniref100_id": "UniRef100_C4XE46", "uniref90_id": "UniRef90_C4XE46" }, "psc": { "uniref90_id": "UniRef90_C4XE46", "product": "Sigma-70, region 4", "uniref50_id": "UniRef50_C4XE46", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [], "id": "IGIGPGHMMC_161", "locus": "GCA000148625_00121" }, { "type": "cds", "sequence": "contig_1", "start": 63719, "stop": 64045, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAACTTAAACCTTTCAGCGAAAATGAATTAAAAGTGTTATTTGAAAAATTCAATGACTATATTGTTGTCGGAGGAATGCCAGAGATTGTTAAAAATTTCATAAAGCAAAAAAATTTCTCAAATATTTTAAATCAACAAAAAGAATTACTTGAAGTCTATAAGCAAGACATTGGTAAATATGCAGAAAGCTTGGATAGATACAAAATTTTAAAAGTTTATGAAAGTATTTCACCATTTTTAGGTAAAGAAAACAAAAGATTTATGTTTTCAAAAGTTGAACATAATGCAAGGTTTAGAGATTATTTTTCAACAATGGAATGA", "aa": "MKELKPFSENELKVLFEKFNDYIVVGGMPEIVKNFIKQKNFSNILNQQKELLEVYKQDIGKYAESLDRYKILKVYESISPFLGKENKRFMFSKVEHNARFRDYFSTME", "aa_hexdigest": "4c92bc2b2be452189879150d33d44091", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12989.88849999999, "isoelectric_point": 9.023849296569825 }, "id": "IGIGPGHMMC_162", "locus": "GCA000148625_00122" }, { "type": "cds", "sequence": "contig_1", "start": 64795, "stop": 64959, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGATATAAAAGAAATTGACAAATTTGGAAAAACTATAACTAGGTTTGAAGATGATGGTCTTTTATATAGTGAAATTTGTATATTTAGAGATTCATTAAGGGAATTCTATAGAAAAAATATAGAAAATAAATTATTCGAATATGAATTATTGAAATAA", "aa": "MKEDIKEIDKFGKTITRFEDDGLLYSEICIFRDSLREFYRKNIENKLFEYELLK", "aa_hexdigest": "031b07ec500201c8b37c84d568d7b608", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6684.602099999996, "isoelectric_point": 5.070288276672362 }, "id": "IGIGPGHMMC_163", "locus": "GCA000148625_00123" }, { "type": "cds", "sequence": "contig_1", "start": 65290, "stop": 65409, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTGTTCCACATGGAAACATTTTAACAAATACAGACAATGGCTTATCTAATAGAAAAATATATGTTTCGAACAAAAATATTTTAAAAGTCGCAGTTCTTAATTGTTTAAATGCTTGA", "aa": "MCVPHGNILTNTDNGLSNRKIYVSNKNILKVAVLNCLNA", "aa_hexdigest": "d0c4bf63fa435b6f899ca1c801fd4865", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4255.9412, "isoelectric_point": 9.30254611968994 }, "id": "IGIGPGHMMC_164", "locus": "GCA000148625_00124" }, { "type": "cds", "sequence": "contig_1", "start": 66046, "stop": 66204, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAATATCTTAATAATTTTGTTAATGAATGCAAAAAAGATAGTGTTAATTACTTAATAATAGATAGATGTGCTGGATCTGGTAATTTAGAAAAAGGACTAAGCACAGAAATACTTAAAAACACTATTTTAAATACGTTTGAATTCTGTGAGTGA", "aa": "MQQYLNNFVNECKKDSVNYLIIDRCAGSGNLEKGLSTEILKNTILNTFEFCE", "aa_hexdigest": "c49098cb6dda08c32d57f160bd22d38c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5962.717499999996, "isoelectric_point": 4.888971900939941 }, "id": "IGIGPGHMMC_165", "locus": "GCA000148625_00125" }, { "type": "cds", "sequence": "contig_1", "start": 66730, "stop": 66888, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTATTTGAATTCAAAATGAACTTTGGTAGGGCAGGCAGCACACTTTATAGTGCATTTGCTGAAGCTTTATCTTATTTAAAGAAATTTAATCAAGATGGTATTAAAAAAATTCCTAGTTCAATATGTATTGCTACTTTTGAAACAAAAAAATATTGA", "aa": "MLFEFKMNFGRAGSTLYSAFAEALSYLKKFNQDGIKKIPSSICIATFETKKY", "aa_hexdigest": "70b2fd4ab214df4f54a05a8c1faa2c79", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5935.885799999999, "isoelectric_point": 9.46442623138428 }, "id": "IGIGPGHMMC_166", "locus": "GCA000148625_00126" }, { "type": "cds", "sequence": "contig_1", "start": 67506, "stop": 68045, "strand": "-", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8LI57", "UniRef:UniRef50_C4XE44", "UniRef:UniRef90_C4XE44" ], "nt": "TTGCATACCTTAAATAAAAAGAATGAATTAATGAAAATAAGTTATTTAAAAAATGAACAAAATGAAAAATCATCTAAAGCTTGGTATGTTTTAACAACAAACAATTCATCTTATGGTCTTTTTGGCTTAAAAAACTATAAAACAGATGTTTTGGAAAAAAATTGTTTTAAAAAATTAAATTTAAAAAATAAATCTTTTATTAATTTTAATAATGACTTTGTACACTCTGCTTATACTTCAGACGATGAATTAAGGTTATTAGAAATATCGGTTAATACTAAAGAAACTGAAATAAAAAATTTTAAAGATTTAAATAAAATTAACAATGAAAATTCGTCCACATCAATGAATAGGTATTACAAAGAAGACTGCGCTGTTTTTTACAATAAAAAAACTAGAATTACATTATTTAATGATAATAAAGAATATATCGCAAAAAGAAAATGTTTAGTTTTTGATTGCATCAGTGAAACACTTTATAGAGCAAATAAAGGTGATGAACTAAACTTTCATAAAATGTTAATAATCGAAAAAAGATAA", "aa": "MHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKLNLKNKSFINFNNDFVHSAYTSDDELRLLEISVNTKETEIKNFKDLNKINNENSSTSMNRYYKEDCAVFYNKKTRITLFNDNKEYIAKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR", "aa_hexdigest": "66af5c6218579b19dc82b1c92f38d462", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8LI57", "query_cov": 0.9497206703910615, "subject_cov": 1.0059171597633136, "identity": 0.7, "score": 234.0, "evalue": 1.72e-76, "valid": false, "uniref50_id": "UniRef50_A0A3P8LI57" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI57" ] }, "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 67506, "stop": 68345, "observations": { "mutation": [ 68240 ], "stop": [ 68240 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE44", "query_cov": 1.0, "subject_cov": 0.5343283582089552, "identity": 0.961, "score": 343.0, "evalue": 5.82e-117, "gene_start": 1, "gene_end": 179, "reference_start": 157, "reference_end": 335, "reference_sequence": "MKLIANKGDIMPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLNWRKVPELHFYIDEVSKKCLSIDRILYDIKDEKNFEANNKISYNEHLTNFGNSKIFELDKYEPQIVLHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKINLKNRSFINFNNDFVHSAYTSDDELRLLEISVNTKKTEVKNFKDLNKINNENSSTSMNRYYKEDCIVFYNKKTRITLFNDNKEYIVKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR" }, "paralog": false, "identity": 0.967741935483871, "score": 548.0, "evalue": 4e-200, "description": "Internal stop codon at 68240. Nonsense mutation around 68240.", "psc": { "uniref90_id": "UniRef90_C4XE44", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_C4XE44", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE44", "UniRef:UniRef50_C4XE44", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE44" ], "product": "Ribosome-binding factor A" } }, "id": "IGIGPGHMMC_167", "locus": "GCA000148625_00127" }, { "type": "cds", "sequence": "contig_1", "start": 68238, "stop": 68483, "strand": "-", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8L789", "UniRef:UniRef90_A0A3P8L789" ], "nt": "ATGCCTAAAAGTATAAACACATTAAGAAGAGAATCACAAATTAGAACATTATTAGCAGAAATAATAACAAATGATTTAACAAATACAAATATTATTAATCCTACATTGGTTGATTGCGAATTATCAGGTGATTTATCACATGTAAAAATCTATATGTCATTTAGCGAAAATGAAAAGAGAGGATTAGAAGCTTTACAAAATGCTTCTGGATACATTAGAACAATTTTGGCTAAAAGTTTAAATTGA", "aa": "MPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLN", "aa_hexdigest": "5888db5fd4d08b4f705c51a66dd6f053", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rbfA" ], "truncated": "5-prime", "pseudogene": { "start": 68085, "stop": 68483, "observations": { "mutation": [ 68240 ], "stop": [ 68240 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8L789", "query_cov": 1.0, "subject_cov": 0.5664335664335665, "identity": 0.889, "score": 142.0, "evalue": 5.04e-42, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MAKNINVLRRESQIRTLLAQIITNDLTNTNIINPTLVDCELSADLSHVKVYMSFSENEKRGLEALQNASGYIRSSLSKSLNWRKVPELHFYIDEVSKKGLSIDKILYNIRDKENFETNNKVSFEQKETNYGNDVNPKTCTKKL" }, "paralog": false, "identity": 0.8333333333333334, "score": 224.0, "evalue": 1.2e-76, "description": "Internal stop codon at 68240. Nonsense mutation around 68240.", "psc": { "uniref90_id": "UniRef90_A0A3P8L789", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A3P8L789", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8L789", "UniRef:UniRef50_A0A3P8L789", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L789" ], "product": "Ribosome-binding factor A" } }, "id": "IGIGPGHMMC_168", "locus": "GCA000148625_00128" }, { "type": "cds", "sequence": "contig_1", "start": 68524, "stop": 68916, "strand": "-", "frame": 2, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43", "UniRef:UniRef90_C4XE43" ], "nt": "ATGAATTTAGTTTCTAATTTATTAAAAATTGGTAAACCTGTTATAGCTCATAGTGACTTTCATATTTTCTTTCCATCCTTAGCTCATGTTACTGAAAATGATGTTGTAATTGTTTACTCAAATAATTTACAAACAATGGAAGCTCACTTTGTTATCGAGCAAGCTAAAAAACAAAAAGCGCCAATTATTGTTTTAACTTCTAGCCAAGAAGAAGATAAATTAATTGCAGTTAAATTAAGATATCACAAAATTCAAAGTTCAACTATGTTAGTTCCTTCAAGTTCAAAAATAGCTCAAATGTTAATTACCGATTTATTATTTGAAGCTGTTTTAGAGCATAATAAGGAAAATACAAGTAAACTTAAAAAAGCAAGAATACTTATTAAAAATTGA", "aa": "MNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKN", "aa_hexdigest": "dcf30baf8efb0cff998da307d29276ff", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpiR" ], "truncated": "both", "pseudogene": { "start": 68494, "stop": 69216, "observations": { "mutation": [ 68526, 69090 ], "stop": [ 68526, 69090 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE43", "query_cov": 1.0, "subject_cov": 0.4377104377104377, "identity": 1.0, "score": 246.0, "evalue": 4.33e-80, "gene_start": 1, "gene_end": 130, "reference_start": 158, "reference_end": 287, "reference_sequence": "MKKFNIITIMKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTIWNDNEVDNDYKFIAASHRYAIDNVYDEDLLKDINKAAEIINKSKNIYIHGCGSSQRISMNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKNWSLIDKAHSE" }, "paralog": false, "identity": 0.9875, "score": 461.0, "evalue": 2.65e-167, "description": "Internal stop codon at 68526, 69090. Nonsense mutation around 68526, 69090.", "psc": { "uniref90_id": "UniRef90_C4XE43", "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE43", "UniRef:UniRef50_C4XE43" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43" ], "product": "RpiR family transcriptional regulator" } }, "id": "IGIGPGHMMC_169", "locus": "GCA000148625_00129" }, { "type": "cds", "sequence": "contig_1", "start": 69088, "stop": 69360, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01418.23" ], "nt": "ATGAAAAATAATGACAAAAGAATAATAAGCCGTCCATTAATGGATGATACAGTTGAATTAACTAAAAGTGAAAAAAGAATTCGAGATTTTATTAATAATTTTGAAGGTAATTATTTTAACTTTTCACAAAATAAGTTAGCTAAAAATACTGAATCAAGTGATGCGGGAGTTTCTAGATTTATTCGTAAATATGGTTTTAAAGACTATCGCACTTTTATTGCTTCAATAAATAACAAATTAACAGAATTTGAAAAAACTTATCCTACTATTTGA", "aa": "MKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTI", "aa_hexdigest": "82241fe3d94061dd2cd7df428f06b7f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01418.23", "name": "HTH_6", "length": 60, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.106326603621472e-11, "score": 39.7202033996582, "start": 18, "stop": 77 } ], "seq_stats": { "molecular_weight": 10671.916599999988, "isoelectric_point": 9.598262596130368 }, "id": "IGIGPGHMMC_170", "locus": "GCA000148625_00130" }, { "type": "cds", "sequence": "contig_1", "start": 69599, "stop": 69766, "strand": "+", "frame": 2, "gene": null, "product": "30S ribosomal protein S16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "nt": "ATGGTTAAAATTAGATTAAAGAGATTAGGAAGCAAATTTAATGCTTGCTACAAAATTGTGGCTGCTGACTCACACGCTCCTAGAGATGGGAAATATATTGAAGCATTAGGCCAATATAATCCACACTCAAAGGAATTTGTTTTAAATGAAGAAGCAACACAAAGATGA", "aa": "MVKIRLKRLGSKFNACYKIVAADSHAPRDGKYIEALGQYNPHSKEFVLNEEATQR", "aa_hexdigest": "78461022c437652e957a87f8934ac54f", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FB42", "query_cov": 0.8727272727272727, "subject_cov": 0.8727272727272727, "identity": 0.688, "score": 75.5, "evalue": 4.6e-17, "valid": false, "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A0A432FB42", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FB42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "product": "30S ribosomal protein S16" }, "genes": [], "id": "IGIGPGHMMC_171", "locus": "GCA000148625_00131" }, { "type": "cds", "sequence": "contig_1", "start": 69839, "stop": 70045, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACGCACACAAAGAAGCTACAAAAGCACAAGAAGTTAAAAAACCTGCAGCTAAACCAGCTACAAAACCTGCTTCAAAATCAACTGCTCCTAAAAAAGTAGTTGCTGACAAAAAAGTTGCAACAAAAGCAGCAACAACTAAAACTACAAAAGCTGCTGCTAAACCTTCAGCTAAAACTGTTCGTAAAACTACAAAAAAATAG", "aa": "MNNAHKEATKAQEVKKPAAKPATKPASKSTAPKKVVADKKVATKAATTKTTKAAAKPSAKTVRKTTKK", "aa_hexdigest": "b9da4fcc3bd7422cb30f67541f4e230e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7099.312000000006, "isoelectric_point": 10.71852970123291 }, "id": "IGIGPGHMMC_172", "locus": "GCA000148625_00132" }, { "type": "cds", "sequence": "contig_1", "start": 70059, "stop": 70760, "strand": "+", "frame": 3, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "RefSeq:WP_013354331.1", "SO:0001217", "UniParc:UPI0001E32F1D", "UniRef:UniRef100_C4XE41", "UniRef:UniRef50_Q98Q98", "UniRef:UniRef90_C4XE41" ], "nt": "ATGAAAATAAATTTTTTAACTCTTTTTTCAAATTACTACAAACCATTTGTAGAAGAATCAATTATTTCAAAAGCTATTGAAAAGAAATTGATTGAAATTAATGTAGTTGATTTTAGACTTTTCAGCAAAGATAAACATCACAAAGTTGATGATGAAATTTTTGGTGGAGGTCATGGAATGCTCCTGCAAATAGAACCTATTGATTTAGCACTTGATTCTCTTAAAGAAAGAGGAGGTTACAAAATTCTTGTAACTCCTCAAGGAAAGAAATTTGATCAACAAATTGCTAATGAATTAGCTAAGAAAAAAGAAATAACTTTTATTTCTGGTCGCTATGAAGGCTTTGATGAAAGAATAGTAGACCTTGTTGATGAAGAAATTTCAATTGGTGATTATGTTTTAACCGGAGGCGAATTACCTTCAATGGTAATGGCAGACAGTATCATAAGATTAGTTCCTGGCGTAATAAGAGAAGAAAGTTATGCTAATGATTCATTTCAAAATGAAGGTTTGTTAGATTATCCTCAATACACTAGACCAAGAGTGTACAAGAGCAAAGCTAAAAAAGAAGCTTTAATAGTGCCTGAAGTTTTAGTTAATGGAAATCATGCAGAAATTGAAAAATGGAAAAAAGAAGCTCAATTAAAAAAGACAAAAAAAAATCGTCCAGATATAATCGAAAGGATTAAAAATGAGAAATAA", "aa": "MKINFLTLFSNYYKPFVEESIISKAIEKKLIEINVVDFRLFSKDKHHKVDDEIFGGGHGMLLQIEPIDLALDSLKERGGYKILVTPQGKKFDQQIANELAKKKEITFISGRYEGFDERIVDLVDEEISIGDYVLTGGELPSMVMADSIIRLVPGVIREESYANDSFQNEGLLDYPQYTRPRVYKSKAKKEALIVPEVLVNGNHAEIEKWKKEAQLKKTKKNRPDIIERIKNEK", "aa_hexdigest": "cf67f528c10da360f56575dc0b732524", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F1D", "ncbi_nrp_id": "WP_013354331.1", "uniref100_id": "UniRef100_C4XE41", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F1D", "RefSeq:WP_013354331.1", "UniRef:UniRef100_C4XE41" ] }, "ips": { "uniref100_id": "UniRef100_C4XE41", "uniref90_id": "UniRef90_C4XE41" }, "psc": { "uniref90_id": "UniRef90_C4XE41", "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_Q98Q98", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q98" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "IGIGPGHMMC_173", "locus": "GCA000148625_00133" }, { "type": "cds", "sequence": "contig_1", "start": 70750, "stop": 71106, "strand": "+", "frame": 1, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_013354332.1", "SO:0001217", "UniParc:UPI0001A8C3B5", "UniRef:UniRef100_C4XE40", "UniRef:UniRef50_O31742", "UniRef:UniRef90_A5IZ68" ], "nt": "ATGAGAAATAAATTAATTGAATTAGTAGAATCAAACCAATTACGTACAGACTTCCCAGAATTCAGAGTTGGAGATAATGTTAAAGTTCACGTTCGTATTCGTGAAGGTGAAAAAGAACGTATCCAAATTTTTGAAGGTTTAGTTATTAGCAAAAAAGAATCAGGTACACGTGAAACATTTACAGTTAGAAAAATTTCTTTTGGTATAGGTGTTAATAGAACATTTCCATTACACTCACCTATGATCGCTTCAATTGAAGTTGTTCGTTCAAATAAAGTACGTCGTGCAAAACTTTACTACATGAAAAACAGAAGTGGTAAAAGTGCTCGTCTTAAGGAAATTAAACGTACAAAATAA", "aa": "MRNKLIELVESNQLRTDFPEFRVGDNVKVHVRIREGEKERIQIFEGLVISKKESGTRETFTVRKISFGIGVNRTFPLHSPMIASIEVVRSNKVRRAKLYYMKNRSGKSARLKEIKRTK", "aa_hexdigest": "1dd843e14e47f79b7c4df38568a503bd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C3B5", "ncbi_nrp_id": "WP_013354332.1", "uniref100_id": "UniRef100_C4XE40", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3B5", "RefSeq:WP_013354332.1", "UniRef:UniRef100_C4XE40" ] }, "ips": { "uniref100_id": "UniRef100_C4XE40", "uniref90_id": "UniRef90_A5IZ68" }, "psc": { "uniref90_id": "UniRef90_A5IZ68", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_O31742", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O31742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O31742" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "IGIGPGHMMC_174", "locus": "GCA000148625_00134" }, { "type": "cds", "sequence": "contig_1", "start": 71269, "stop": 71403, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGGAGTTTCATATATTGAACTTTTAGTTGATGGTAAAACTGTAGATAAATTAAATGTTTTTAAGAAAAGAAATGCTTCATTAAGTCATAATTTAGATGGCGACCTAATTCATGTTAGAATTGATGGCTGA", "aa": "MVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDG", "aa_hexdigest": "0cd091e3115818f9704f451dec542a25", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4880.5796, "isoelectric_point": 6.700998878479004 }, "id": "IGIGPGHMMC_175", "locus": "GCA000148625_00135" }, { "type": "cds", "sequence": "contig_1", "start": 71470, "stop": 71664, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39", "UniRef:UniRef90_C4XE39" ], "nt": "ATGTCTGAAAAAGGAAAAAAATTTAAAAAGAAACGTACAGGAATGATAATTGCTAGTTTAATATTTCTTTTTATAAGTATTGGATTAGCTGGAGCATTATTTCCAATAATTCATATAGTTGTTACAACTAAAAATTCAATTGGTTCAATAATAGCCATTATCATATCAGCTGTTTTTTTCATAATAACAATTTGA", "aa": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITI", "aa_hexdigest": "9d77ab5e6f8731718071735c0a82944e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 71224, "stop": 71664, "observations": { "mutation": [ 71271, 71472 ], "stop": [ 71271, 71472 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE39", "query_cov": 1.0, "subject_cov": 0.4383561643835616, "identity": 1.0, "score": 108.0, "evalue": 6.46e-29, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITIWFINFSIGTNYKVVNYKDKTIEVLVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDGWVFYQVNIKINNELL" }, "paralog": false, "identity": 0.9794520547945206, "score": 272.0, "evalue": 5.3e-96, "description": "Internal stop codon at 71271, 71472. Nonsense mutation around 71271, 71472.", "psc": { "uniref90_id": "UniRef90_C4XE39", "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE39", "UniRef:UniRef50_C4XE39" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6935.540999999996, "isoelectric_point": 10.751730918884277 }, "id": "IGIGPGHMMC_176", "locus": "GCA000148625_00136" }, { "type": "cds", "sequence": "contig_1", "start": 71699, "stop": 72145, "strand": "-", "frame": 1, "gene": null, "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_013354334.1", "SO:0001217", "UniParc:UPI0001E32F40", "UniRef:UniRef100_C4XE38", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_C4XE38" ], "nt": "ATGCATAAGATTAAAATAATTGCAGTTGGTTCACTTAGTCCAGAATTTAAAGTTTTATTTAATCAATATGCTAAAAACATAAGTCATTTTGCTGAATTAAATGTTGTTGAAGTAAAAGAATTGAGTGAGGAAAAAAATATCGAAGTCAAGAAAGACAAGGAAACAAAATTAATTAATTCTTATGTTGATAATAATAGTTTTGTTATTTATGCTTCATTAAAAGGAAAAAGTTTAGATTCAGTTGAATTAAGCGAAATAGTTAATAACAATGTTAATTTAACCTTTATAATTGGCGGCAGTGATGGAGTTAATGAAGAATTAATTAAATGCAATATGAAAATTAATTCTTCAAAATTAACTTGGCCACATCAATTGTTTAGAGTTATGTTAGCTGAACAAATCTATCGTTCATTTATGATCATTAATAATAAAAAGTATCATAAATAA", "aa": "MHKIKIIAVGSLSPEFKVLFNQYAKNISHFAELNVVEVKELSEEKNIEVKKDKETKLINSYVDNNSFVIYASLKGKSLDSVELSEIVNNNVNLTFIIGGSDGVNEELIKCNMKINSSKLTWPHQLFRVMLAEQIYRSFMIINNKKYHK", "aa_hexdigest": "5a24f8abde1e52ce51a1ab84206930eb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F40", "ncbi_nrp_id": "WP_013354334.1", "uniref100_id": "UniRef100_C4XE38", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F40", "RefSeq:WP_013354334.1", "UniRef:UniRef100_C4XE38" ] }, "ips": { "uniref100_id": "UniRef100_C4XE38", "uniref90_id": "UniRef90_C4XE38" }, "psc": { "uniref90_id": "UniRef90_C4XE38", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [], "id": "IGIGPGHMMC_177", "locus": "GCA000148625_00137" }, { "type": "cds", "sequence": "contig_1", "start": 72138, "stop": 73991, "strand": "-", "frame": 3, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "GO:0002098", "GO:0008033", "KEGG:K03495", "RefSeq:WP_013354335.1", "SO:0001217", "UniParc:UPI0001E32F41", "UniRef:UniRef100_UPI0001E32F41", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_C4XE37" ], "nt": "ATGAATAAGAAAAGTAATAATGTACATAAATATGATGCTGTTGTTATAGGTGGAGGGCACGCTGGTTTAGAAGCTGCTTTTGCCTTAGCACACAAAAATCATAAAACTGTTTTGATATCTTTAAATAAGGAACGTTTAGGTATGATGCCATGTAATCCTTCAATTGGTGGACCAGCCAAAGGAATCATAACTAGAGAAATAGATGCCTTAGGCGGAATGCAAGGACTTTGGGCTGATTTAAGCATGATCCAATTGAAAATGCTTAATCAATCAAAAGGTCCTGCAGTTAGAGCACTTAGAGCTCAAATTGACAAAGAAAAATACATGAAGATAGCTTTAGAATACGTGTTAAAAGAACCTAACTTAGATCTTATTGAAGATATAGCTGAAGAAATATTAGTAAATAGTAAAAAAGAATTTAAAGGAATTAAACTAGCTAATGGCGATATAGTTGAAGCTGAGGTCTGTGTTGTAACTACTGGAACATATATGGACAGTAGAATTCTTAGAGGAGATGTTATTAAAATCAGTGGACCTGATAATGAAAAAACTACACCTAAATTAAGTGCTTCATTAAAAGAACATGGTTTTATAATTCAAAGACTTAAAACCGGAACACCTCCAAGAATCTTTTCAGATTCAATTGACTACTCGCAAGTAGAACAAGAAGTATTAAGTGATACTAACTTATCATTTTCATCTAGATCAAATGTAAAATTACCTCGTCAAATTGCTTGTTACTTAACTTATACAACTCCTGAAACTCACAAAATAATAGAAGAAAATTTAACTAAAAGTGCTATGTATGGAGGAATAATCAAAGGTATTGGCCCTCGTTATTGTCCTTCAGTTGAAGACAAAATTGTTAAGTTTCCTTCAAAAGAAAGACATCAAATTTTCTTCGAACCTGAAACTGCTGATGGTTCAATTACTTATGTTAATGGGCTCTCTACTTCAATGCCAGTTGAAGTCCAAGAACTAATGATTAGAAGTATTCCTGGTCTTCAAAAATGTAAGATTCAAAAGTATGCTTATGCTATTGAATATGATGCAATTGATCCTTTGCAATTAAAACCTACTTTAGAAACTAAAATCATTAAAAATTTTTATACTGCAGGTCAAATTAATGGAACTAGTGGTTACGAAGAGGCTGCTGCTCAAGGTTTAATTGCAGGTATTAATGCAGCCTTAAACTTAGAAAAAAAAGATGGTATAGTTATTTTAAGAAACCATGCTTATATAGGAGTTTTAATTGATGATTTGGTTACTAAAGGAACTAAAGAACCTTATAGAATGCTAACTTCAAGAGCTGAATATAGATTACTTTTAAGAAATGATAATGCTGATATTCGTTTAGCCGAATATGGCTATAAAATAGGCTTAATTGACAAACATCAATATCAAAAGGTTATTAAAAAATATGAATTAATTGACCAAGAAATTCAAAGACTTCATACTACTTATCTTTCTTCAAAAGATCCTGTGGCTATAAAATATGGAATCACAACAGGTATCTCATTATTACAAACTTTAGCTCGTCCTGCAGTTGATCCTTATGATATCATTCATGATTTTCCATACCTAGAAGAATTAACAACAATGGTTAGACTTCATGGTTATATTGAAAAACAAAAAAGTGATGCTAATAAAGCTGTAAGACTAGAAAATTACAAAATACCAGAAGACTTGGACTACAACAAAATTAATAATATTGCTACAGAAGCTAAACAAAAATTAATCAAAGTTAAACCAACAACAATTGGCCAAGCAAGTAGAATCAGTGGCATTAATCCAGCTGATATTCAAATGCTTATGTTTTATTTAGAAACTACAAGAAAAAAGAATAATGCATAA", "aa": "MNKKSNNVHKYDAVVIGGGHAGLEAAFALAHKNHKTVLISLNKERLGMMPCNPSIGGPAKGIITREIDALGGMQGLWADLSMIQLKMLNQSKGPAVRALRAQIDKEKYMKIALEYVLKEPNLDLIEDIAEEILVNSKKEFKGIKLANGDIVEAEVCVVTTGTYMDSRILRGDVIKISGPDNEKTTPKLSASLKEHGFIIQRLKTGTPPRIFSDSIDYSQVEQEVLSDTNLSFSSRSNVKLPRQIACYLTYTTPETHKIIEENLTKSAMYGGIIKGIGPRYCPSVEDKIVKFPSKERHQIFFEPETADGSITYVNGLSTSMPVEVQELMIRSIPGLQKCKIQKYAYAIEYDAIDPLQLKPTLETKIIKNFYTAGQINGTSGYEEAAAQGLIAGINAALNLEKKDGIVILRNHAYIGVLIDDLVTKGTKEPYRMLTSRAEYRLLLRNDNADIRLAEYGYKIGLIDKHQYQKVIKKYELIDQEIQRLHTTYLSSKDPVAIKYGITTGISLLQTLARPAVDPYDIIHDFPYLEELTTMVRLHGYIEKQKSDANKAVRLENYKIPEDLDYNKINNIATEAKQKLIKVKPTTIGQASRISGINPADIQMLMFYLETTRKKNNA", "aa_hexdigest": "c7771d98800428902df6e0b356ed9086", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F41", "ncbi_nrp_id": "WP_013354335.1", "uniref100_id": "UniRef100_UPI0001E32F41", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F41", "RefSeq:WP_013354335.1", "UniRef:UniRef100_UPI0001E32F41" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F41", "uniref90_id": "UniRef90_C4XE37" }, "psc": { "uniref90_id": "UniRef90_C4XE37", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "IGIGPGHMMC_178", "locus": "GCA000148625_00138" }, { "type": "tRNA", "sequence": "contig_1", "start": 74054, "stop": 74130, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.4, "nt": "GCGGATGTGGTGAAGTGGTCTAACACAGCGGGTTGTGGTCCCGTCATTCGAGGGTTCGAATCCCTTCATCCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 74060, 74062 ], "id": "IGIGPGHMMC_4", "locus": "GCA000148625_00139" }, { "type": "cds", "sequence": "contig_1", "start": 74314, "stop": 74748, "strand": "+", "frame": 1, "gene": null, "product": "DHH family phosphoesterase", "db_xrefs": [ "EC:3.1.13.3", "EC:3.1.3.7", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_A0A449B7A5", "UniRef:UniRef90_C4XE36" ], "nt": "ATGAAAATAGGAAATTATAAAGTTGCATTACAAGCAATCGAAAAATATAAGAATATTATTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATAAAAAAGAATTATCCAAAGAAAAAAGTTTTTACAGTTGGAGATAATGTTCATACTTTTGATTTTATGAGTTACCACTATGACCCAATTGAAACGGTTGATTTTAAAAATTCATTAGGTATTGTAGTTGATGCTTCAAGTAGTGATCGGATTCAAGATGGACATTTATTAGTAGAAAAAAAATGTACAGCTGCTTTAAGAATTGATCACCATCCAAATGGTTCAGATATCGAATATGATTATTTATGGATCGATGAACACTATGTAGCTGCGGCTGAAATGGTGGCTATGTTAGCTAAAAGTGCAAAATGA", "aa": "MKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAK", "aa_hexdigest": "323886bd708bbedc1b7ebd8def08d762", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 74287, "stop": 75051, "observations": { "mutation": [ 74746, 74887 ], "stop": [ 74746, 74887 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE36", "query_cov": 1.0, "subject_cov": 0.4350453172205438, "identity": 1.0, "score": 297.0, "evalue": 2.09e-99, "gene_start": 1, "gene_end": 144, "reference_start": 10, "reference_end": 153, "reference_sequence": "MNIKLKEKNMKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAKWEITKKAAEHIYLGINTDSGRFLYPDTSARTYKLVAHLIEKGGLDSQWILRELNKRSSRDIKYTGEILSGFKQSGRVLYFEVTKEIMDKYGMNDLEAAIFVNELANIDDNSCWAFFIQLADGTVRCRLRSNGPLVNNVARLHNGGGHDNAAGCSINSWDEVPVVLKELNDCIKEWEAK" }, "paralog": false, "identity": 0.9881889763779528, "score": 503.0, "evalue": 8.08e-183, "description": "Internal stop codon at 74746, 74887. Nonsense mutation around 74746, 74887.", "psc": { "uniref90_id": "UniRef90_C4XE36", "product": "DHH family phosphoesterase", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_A0A449B7A5", "kegg_orthology_id": "K06881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE36", "EC:3.1.13.3", "EC:3.1.3.7", "UniRef:UniRef50_A0A449B7A5", "KEGG:K06881" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7A5" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase nrnA" } }, "id": "IGIGPGHMMC_179", "locus": "GCA000148625_00140" }, { "type": "cds", "sequence": "contig_1", "start": 75282, "stop": 75665, "strand": "+", "frame": 3, "gene": "nrnA", "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "nt": "ATGGGACACATGAAAATTGGTAGCTTAAAAGAAGCAACAAAATTAATTAAAAAATATGACTCAATAGTTATTTTTCATCACATTCGTCCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATTAGAGCTAATTTTCCAGAAAAGAAAGTTTACACAGTTGGCGATAATGAAGGTATGTTTCCATTTTTAAATCTTAAATATGATAAATGTCCTTCAGATAATGTACTTAAAAAATCTTTAGGCATTATTCCGGATGCAAATGGTAAAGAAAGAATTCAATGTCGAGAAATTTTAGATAAAAACTTGTTTGCTGAAACACTTAGAATTGATCATCATCCAAATGGTGATGACTTAGATAAATGTACCAGATGA", "aa": "MGHMKIGSLKEATKLIKKYDSIVIFHHIRPDGDCLGSQAGLAELIRANFPEKKVYTVGDNEGMFPFLNLKYDKCPSDNVLKKSLGIIPDANGKERIQCREILDKNLFAETLRIDHHPNGDDLDKCTR", "aa_hexdigest": "1ef3fc7e774a6e89631548d43958c6ca", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024C6B900", "query_cov": 0.984251968503937, "subject_cov": 0.8741258741258742, "identity": 0.6559999999999999, "score": 175.0, "evalue": 3.75e-54, "valid": false, "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "uniref50_id": "UniRef50_UPI0024C6B900" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C6B900", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "IGIGPGHMMC_180", "locus": "GCA000148625_00141" }, { "type": "cds", "sequence": "contig_1", "start": 75921, "stop": 76145, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATAATTTAAAAACAGTTGGTAAAGTTGCTTATACAACTATTGATTTAAAAACAACAAAAGCTTTAGGCAAAAAACCAAATCAAGTTGTTAGAGTAAATACAATTGGGAATATTATTGGTTATCCATTTTGGGTTCAATTCAATGAAGAAGAAAATGGCAATGTTAGAATTGAATTTAGATCAAGTGGTCCTTGTGTTAGAAATGTTGCAGTTAAATGA", "aa": "MLNNLKTVGKVAYTTIDLKTTKALGKKPNQVVRVNTIGNIIGYPFWVQFNEEENGNVRIEFRSSGPCVRNVAVK", "aa_hexdigest": "2970673e9479e747f725cac31bca1e1e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8280.499499999998, "isoelectric_point": 9.917961311340331 }, "id": "IGIGPGHMMC_181", "locus": "GCA000148625_00142" }, { "type": "cds", "sequence": "contig_1", "start": 76483, "stop": 76755, "strand": "+", "frame": 1, "gene": "rimP", "product": "Ribosome maturation factor RimP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34", "UniRef:UniRef90_C4XE34" ], "nt": "TTGTTAGACATTAGTGTCAATTATCATGACTTAAATAAGGTTGAAGAAATATCAAAACAACTTAATGAATTTATAGATTCTAATTATCCATCTTTGGATATTGATTCATTATCAATACATTCTCCAGGTGTCGAATTAACCTATAAAACAGATGAATTAGGAAAACACATTGGAGAAATGTTAACTATAAAATTAAATAAAAGTATTGAAAAACAAGACAAATTTAATGGTAAATTATTAGAAGTTTTAGAAGGCAAAATTCAAATAAGTTGA", "aa": "MLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQIS", "aa_hexdigest": "91f3260c4d36865ff043a97367d2ffa2", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "rimP" ], "truncated": "both", "pseudogene": { "start": 76204, "stop": 76836, "observations": { "mutation": [ 76327, 76348, 76408, 76753 ], "stop": [ 76327, 76348, 76408, 76753 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE34", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.9890000000000001, "score": 173.0, "evalue": 3.89e-53, "gene_start": 1, "gene_end": 90, "reference_start": 94, "reference_end": 183, "reference_sequence": "MQLLKTVMRKLNVEKEKATTTKKNNFKTCTSASFFVDLKILWYNKTIKWILQSCNLQLFVIFVGGFMNWKEILEKQFGSQILEAKIIEEDDLKLLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQISWNNKGQFRKVFIDKNNIVKVEKYIKFN" }, "paralog": false, "identity": 0.9809523809523809, "score": 397.0, "evalue": 4.59e-144, "description": "Internal stop codon at 76327, 76348, 76408, 76753. Nonsense mutation around 76327, 76348, 76408, 76753.", "psc": { "uniref90_id": "UniRef90_C4XE34", "product": "Ribosome maturation factor RimP", "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE34", "UniRef:UniRef50_C4XE34" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34" ], "product": "Ribosome maturation factor RimP" } }, "id": "IGIGPGHMMC_182", "locus": "GCA000148625_00143" }, { "type": "cds", "sequence": "contig_1", "start": 76912, "stop": 77193, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "TTGATAGGTTATTTTGAAAAAGAAAAACTTCCTATTGAAGAAGTTATCAAAATTTTTTCAGAAGAAACTACAAGAATTATTAACAAAATCTTAGATCCAAATGCTGAAATAGTTTTTGAATTAGACAAAGAAAAACAAGAAGTTCACATTTTCAACAATAAAACATGTGTAACTAAAGATGAAGAATTATTGCTTGATCTTGAAAATCAAATAAGTTTTGTTGGATATAAAGAAGCAAAAGTAAGAATGTCTAAAGTTAAAGAAGATGATGAAATTAAATGA", "aa": "MIGYFEKEKLPIEEVIKIFSEETTRIINKILDPNAEIVFELDKEKQEVHIFNNKTCVTKDEELLLDLENQISFVGYKEAKVRMSKVKEDDEIK", "aa_hexdigest": "6e02169728fa5d45391cde47d19b6e10", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 88, "aa_cov": 0.9354838709677419, "hmm_cov": 0.9204545454545454, "evalue": 1.046652200254861e-06, "score": 24.97865867614746, "start": 6, "stop": 92 } ], "seq_stats": { "molecular_weight": 10991.526099999995, "isoelectric_point": 4.7830808639526365 }, "id": "IGIGPGHMMC_183", "locus": "GCA000148625_00144" }, { "type": "cds", "sequence": "contig_1", "start": 77395, "stop": 77553, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAATGTTAATGCATATTTACCAGCAGGATTAATTAGTACAAAAAGAAAAGCAAACCCAGGTCAATATTTTGATGTTGTTATTGAAAAAGTTTCAGAAGAAACAAAATTGAGTCAAATTGAAGTTTCATTAGACTCACCTAAAATTGTTTGA", "aa": "MNSNVNAYLPAGLISTKRKANPGQYFDVVIEKVSEETKLSQIEVSLDSPKIV", "aa_hexdigest": "2af832be75027ba1af4df14ab9309e20", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5722.4785999999995, "isoelectric_point": 6.090263938903808 }, "id": "IGIGPGHMMC_184", "locus": "GCA000148625_00145" }, { "type": "cds", "sequence": "contig_1", "start": 78196, "stop": 78462, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCATTACAAGAAGATGACTCAAACTTCGAAGAATTAGTTAAGAAAATTGAAAGCGAATATGCAGTTGAAAATGAAGTAGAAAACATTAAAAATGTTGAAGTAACAAAACCAGTTGCTCAAGATAACAAAATTTCCGCATCTGACTATAAAAAAGCTAAAGAAGTCGCAAAAGACTTTGTTGTTGATGATGATTTAAAGAATTTTGGAATTGACGATTTTGATTTAAGTGACATTGATTTTGATGAAGAAGAAAACAAATAA", "aa": "MDSLQEDDSNFEELVKKIESEYAVENEVENIKNVEVTKPVAQDNKISASDYKKAKEVAKDFVVDDDLKNFGIDDFDLSDIDFDEEENK", "aa_hexdigest": "1994556343f9dcef3bd3cc6ad8fea579", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10124.812699999997, "isoelectric_point": 4.052472496032714 }, "id": "IGIGPGHMMC_185", "locus": "GCA000148625_00146" }, { "type": "cds", "sequence": "contig_1", "start": 78736, "stop": 80541, "strand": "+", "frame": 1, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_A0A3P8LI10" ], "nt": "ATGCCTAGAAAGAACAGAATAAGTAACGTTGATGAAGTCAAGAACCAACTTTCAGATATTAAAACTGAAGTTAAGAATGGTGTTTTCATTTTTACAAACAAAATGCCATTAGGAGAATTTGCACAAAAAATTGGTATGAATGCCAATGATTTGATCAAGCGATTTTTGATGAAAGGAAAGCTTTATCAAATTAACTACGTACTTGATGAAGAAGAAATTGCTGAAGTATGTATGGAAAGAGGACTTGACTTTAGAAAAGAACAAAACATTGATGCTGCAAATTTTTTGCATGCAGTTAAATTTGAAGATGATGAAAAATCATTAGTAAAAAGACCTCCAATTATTACAGTTATGGGTCATGTTGATCACGGAAAAACTACATTAATTGACTTTATTAGAAAAACTAAAGTTGCTGAAACTGAAAGTAGTGGTATTACTCAACATACTGGTGCTTACCAAGTAGTTTACAAAGGCAACAAAATTACATTTATTGACACTCCTGGACATGAAGCATTTACCGAAATGCGTTCACGTGGAGCTAAAATTACAGACATTATTATTTTAGTAGTAGCTGCTGATGATGGTGTTATGCCACAAACTAGAGAAGCTATTGACCACGCAAAAGCAGCTGATGTTCCTATCGTTGTTTTTGTAAATAAAATAGATAAACCAAATAAGGATCTTGAAAGAATTAAAAGAGAATTAGCTGAATGCAACGTTATTGTTGAAGAATATGGTGGTGACGTTCAAATGGTAACTGGTTCTGCTTTAAAAGGTCAAGGTATTGAAGATTTATTTGAAGCAATTAGTTTACTTGCTCAATTATTGGATTTAAAAGCCAACCGTTCAAGATATCCAGTTGGTACTGTTATTGAAAGTAAAATAGATAAAGGTGTTGGTGTTGTTTCAACTTTAATTGTTGAAAACGGTAGCTTATATAAAGGAGACTTTATTGTAGCTGGCTCAAGTTATGGGCGTGTGCGTTCATTAAAAGATGCTACTGGAAAACAAATGGAACATGCTCACCCTGGAACACCAGTTGTTGTTACTGGATTAAATAATTCGCCACTTGCAGGTGATAGATTTATTGGTTTTCAAGATGAAAAATTTGCTAAAAAATTAGCTGAAGAAAAAGCTCAAATCGATAAGAACAGAGCTTTATATGACAAGAGCCAAACTTCAGAAATTGAAATTGGCAAAAAGGTATTTAATGTAATCATTAGATCAGATGTTCAAGGAACAGCTGAAGCTATCAAAGGCAAAATAGATGGCATGTGTAATGATGAAGCAATGATTAAAGTTATTGGCGCTCAAGCGGGACAAATAACAAATGCTGACTTATTATTAGCTCAAGCCTCAGATGCAAGGATTATAACTTTCAATGTTAAGCCATCTGCAGCTATTAAGCAAAGTGCAAAACATTCGGGAATTACAATTGTTAATTATTCTGTTATTTACAAAATTATTGAAGATATGGAAGCTCACCTTAAGGGTCAAAAAGCAGTTGTTTATGAAGAAAAGAAAATCGGATCAGCTCATATTATCAAAGTTTTCTTCTATTCAAAAGTTGGAGCAATTGCTGGATGTATGATGGATGAAGGCGTTGTAAAAGCTAATTGTAAAGTTAAAGTTTTTAGAGGCAGAAAAATGATTCATGATGGTGTAATTGAAACGCTTAAACGTGAATTAAATGATTCAAAAGAAGTTGAAAAAGGTAAAGATTTTGGTACTCACATTAAGAAGTTTGATGATATCAAGGAAGATGATATTTTAGAATTCTTTGAAGATGTTCCAGTCTCTTTATAA", "aa": "MPRKNRISNVDEVKNQLSDIKTEVKNGVFIFTNKMPLGEFAQKIGMNANDLIKRFLMKGKLYQINYVLDEEEIAEVCMERGLDFRKEQNIDAANFLHAVKFEDDEKSLVKRPPIITVMGHVDHGKTTLIDFIRKTKVAETESSGITQHTGAYQVVYKGNKITFIDTPGHEAFTEMRSRGAKITDIIILVVAADDGVMPQTREAIDHAKAADVPIVVFVNKIDKPNKDLERIKRELAECNVIVEEYGGDVQMVTGSALKGQGIEDLFEAISLLAQLLDLKANRSRYPVGTVIESKIDKGVGVVSTLIVENGSLYKGDFIVAGSSYGRVRSLKDATGKQMEHAHPGTPVVVTGLNNSPLAGDRFIGFQDEKFAKKLAEEKAQIDKNRALYDKSQTSEIEIGKKVFNVIIRSDVQGTAEAIKGKIDGMCNDEAMIKVIGAQAGQITNADLLLAQASDARIITFNVKPSAAIKQSAKHSGITIVNYSVIYKIIEDMEAHLKGQKAVVYEEKKIGSAHIIKVFFYSKVGAIAGCMMDEGVVKANCKVKVFRGRKMIHDGVIETLKRELNDSKEVEKGKDFGTHIKKFDDIKEDDILEFFEDVPVSL", "aa_hexdigest": "60cac5f5d911a94572a605c4c1f27ba7", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LI10", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.998, "score": 1160.0, "evalue": 0.0, "valid": true, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "IGIGPGHMMC_186", "locus": "GCA000148625_00147" }, { "type": "cds", "sequence": "contig_1", "start": 80571, "stop": 81086, "strand": "+", "frame": 3, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_013354341.1", "SO:0001217", "UniParc:UPI0001E32F47", "UniRef:UniRef100_UPI0001E32F47", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_Q9RFQ2" ], "nt": "ATGGATTTAAAAAAATATATTCGAGATATTGAAAATTTTCCTAAAACAGGCATAATGTTTAAAGATATTTCACCATTATTAGCAAATGGTAAAGCATTAAATTATACAATCATAGAAATGTCAAAATTAGCTCAAGATGTTGATGTTATTGTTGGACCAGACGCGAGAGGATTTTTATTTGGCACGCCTACTGCAGCATTTTTGAAGAAACCATTTATTATGGTTAGAAAACCAGGAAAATTACCTGGTGATGTTATTTCAAAAAGTTACGATTTGGAATATGGAAATAATGTTTTACAAATTCAAAAAGGGTTTATTAAAGAAGGTCAAACAGTTGCAATTATTGATGATGTTTTAGCAACAGGTGGAACAACTAAAGCTATTATAAAACTATTAGAAGAACAAGGTGCGATTGTTAAAAAAGTTATTTTACTTCTTGAATTAGTAGATCTTAATGGTAGACAATTAATCACTAAAGATAATGATATTGAAATAGTTTCCTTAGTTAAATTTTAA", "aa": "MDLKKYIRDIENFPKTGIMFKDISPLLANGKALNYTIIEMSKLAQDVDVIVGPDARGFLFGTPTAAFLKKPFIMVRKPGKLPGDVISKSYDLEYGNNVLQIQKGFIKEGQTVAIIDDVLATGGTTKAIIKLLEEQGAIVKKVILLLELVDLNGRQLITKDNDIEIVSLVKF", "aa_hexdigest": "12e467ec72fbf73e069eb76e4c9e5fbe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32F47", "ncbi_nrp_id": "WP_013354341.1", "uniref100_id": "UniRef100_UPI0001E32F47", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F47", "RefSeq:WP_013354341.1", "UniRef:UniRef100_UPI0001E32F47" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F47", "uniref90_id": "UniRef90_Q9RFQ2" }, "psc": { "uniref90_id": "UniRef90_Q9RFQ2", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "IGIGPGHMMC_187", "locus": "GCA000148625_00148" }, { "type": "cds", "sequence": "contig_1", "start": 81156, "stop": 81341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGATAATCGAAAGCAGAATCGCAAATAATAAAATTACATTTTTCTTCAGTGGCATGAACAAAAAAGAATTTGAATCTTACTACCATGATCAACTTAAAAGAGAGCCTTACAAAGTTAAAACTTTTACTTCTCGAATTCTTAATAACACACTTGAATTTTGCATTGATGAAATTGAAAAATAA", "aa": "MEIIESRIANNKITFFFSGMNKKEFESYYHDQLKREPYKVKTFTSRILNNTLEFCIDEIEK", "aa_hexdigest": "af2a623284fc58496cd29cefca5b5ed6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.4203, "isoelectric_point": 6.563391685485839 }, "id": "IGIGPGHMMC_188", "locus": "GCA000148625_00149" }, { "type": "cds", "sequence": "contig_1", "start": 81360, "stop": 81668, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF00308.24", "PFAM:PF01695.23" ], "nt": "ATGTTTGAATATATTAATCATCTAAAAGTTGCAATAGCTAAAAAGAAACAAAAAGGACTAAAAGGCATTTATGTCTATGGAGCTAACAACACTGGCAAAACTTACATTGCTAGTGCAATAGCCAACGAATTTGCAGCTGAAGGTATTTCATCAGTTTATTTAACAACCAGTGCTTTATATTCATTCTTAATTTCAAACATGGGTAGCAATGCTACAAATGCTAATGCAGTTATTGACATAATTAACAAACTCAAAAAAGTTAATGCATTAATTATTGACGAAATTGGTTTAGAAAAAAATAACTACTGA", "aa": "MFEYINHLKVAIAKKKQKGLKGIYVYGANNTGKTYIASAIANEFAAEGISSVYLTTSALYSFLISNMGSNATNANAVIDIINKLKKVNALIIDEIGLEKNNY", "aa_hexdigest": "cdc3287e94fa236ed8c6793449701b29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 73, "aa_cov": 0.7156862745098039, "hmm_cov": 0.9041095890410958, "evalue": 4.404579407573133e-08, "score": 29.68245506286621, "start": 23, "stop": 95 }, { "id": "PF00308.24", "name": "Bac_DnaA", "length": 89, "aa_cov": 0.8529411764705882, "hmm_cov": 0.9438202247191011, "evalue": 7.965021322386773e-07, "score": 25.41262435913086, "start": 9, "stop": 95 }, { "id": "PF01695.23", "name": "IstB_IS21", "length": 76, "aa_cov": 0.7450980392156863, "hmm_cov": 0.9342105263157895, "evalue": 2.771254462148612e-08, "score": 29.561506271362305, "start": 23, "stop": 98 } ], "seq_stats": { "molecular_weight": 11094.677899999986, "isoelectric_point": 9.30196590423584 }, "id": "IGIGPGHMMC_189", "locus": "GCA000148625_00150" }, { "type": "cds", "sequence": "contig_1", "start": 81744, "stop": 82049, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAAAAGAAGAAATTAAAAATGAGTTATTTTTTAATTCCAAGGCTGAATTTGAAAACAAAGCACTTGAAATAATGCATGGCCATCCGCAACTAGAAGCATTAATTCAAGCTAACAATATTACAAATGAAGAAATTTTGGAAAACTTATTTACATTTATTAATATTAAAGAATCATTGGATAATTCTCAAATTTATCCATGGATTTTTTCTATTTCAAGAAAAAATGAAAAATTAGTTTTTAAAAAGATTGCTGCAAAAAATGAATACAAATCAGTTGTTACACGACACATCAATTTATGA", "aa": "MAAKEEIKNELFFNSKAEFENKALEIMHGHPQLEALIQANNITNEEILENLFTFINIKESLDNSQIYPWIFSISRKNEKLVFKKIAAKNEYKSVVTRHINL", "aa_hexdigest": "389e0559edf23678371fb16b54db587f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11823.435999999983, "isoelectric_point": 6.295850563049316 }, "id": "IGIGPGHMMC_190", "locus": "GCA000148625_00151" }, { "type": "cds", "sequence": "contig_1", "start": 82063, "stop": 83025, "strand": "-", "frame": 2, "gene": "dnaD", "product": "DnaD domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354343.1", "SO:0001217", "UniParc:UPI0001E32F48", "UniRef:UniRef100_UPI0001E32F48", "UniRef:UniRef50_N9V249", "UniRef:UniRef90_C4XE27" ], "nt": "ATGCTAAAAAATATTCCTTACGCATTCTTCAGAATCAAACATTCTTCATCAGTTAGTGGAGAAGATTTAAAAAACTTACGCAAGTTTTACGCTCCTATTTTAGGTAGTGATGCAATTTTGCTTTATGAATACCTTAGAGATCTATCATTTTCTCAATCTCCAGAAACTGGTTTTTATGACTATGATAGCCTTACTTATCTATTAAATATGGATGTAAATAAACTAAATCAAGCACGTATAAAATTAGAATCTGTATCTTTAATTTCTACTTTTATAGATGAGTTTAATCGCAAAACACTTTTTGTTATTGAAAAACCACTTGATAATACAGGTTTCAAAAAGAATTTAATTCTAGCAAACAAACTCATTAGTATTATTGGTAGCAAAAACTTTGAAAGATTAGTTGGACGTGAAAGAAATATTTATTTAAGTAAAGCTTCAAATCTTTTGGATGCTTCAGCTTCTTTTGAAGAAGTTTTTCCTGAACCAAGTGAAGAATTAACTATTACTTTTTCACATGAAGTTGACTCAAGTATGATTAAAACTCAAGAAATAGTTGAAGAAATTCAAACCAAATTGTCACTTAATACTTTTGATTATCCTAATCCTTATGAAGCAATACTTAAAACTGATTCAAGATACTTCTATTCTCAAATTAGTCACAAAATACCACGTCAAAATGTTTTAGATTTAATTAAAGAAAGTAGAGCAGCTGGCTTAGGTGATCCTTGCTTAAACTTAGTATTCTTTTATTCAAATGAAATAAATAATTGCATAAATACCAATTATGTGGGTAAAATTATTAAGGATTTAATTAAAAAAGAAATCTTCTCATTTGAAGCAATTGAAAATTATTTAGATGACATACTTAAAGTTAAAAACAAAATTGCAGTTTCAAAAATTAATTTATATAAAGCTACCTATTTAGAATCACTTCAAAGAGAAGACAACAACAATATTTAA", "aa": "MLKNIPYAFFRIKHSSSVSGEDLKNLRKFYAPILGSDAILLYEYLRDLSFSQSPETGFYDYDSLTYLLNMDVNKLNQARIKLESVSLISTFIDEFNRKTLFVIEKPLDNTGFKKNLILANKLISIIGSKNFERLVGRERNIYLSKASNLLDASASFEEVFPEPSEELTITFSHEVDSSMIKTQEIVEEIQTKLSLNTFDYPNPYEAILKTDSRYFYSQISHKIPRQNVLDLIKESRAAGLGDPCLNLVFFYSNEINNCINTNYVGKIIKDLIKKEIFSFEAIENYLDDILKVKNKIAVSKINLYKATYLESLQREDNNNI", "aa_hexdigest": "081aade39ee62706cd60cd7f9acc6927", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32F48", "ncbi_nrp_id": "WP_013354343.1", "uniref100_id": "UniRef100_UPI0001E32F48", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F48", "RefSeq:WP_013354343.1", "UniRef:UniRef100_UPI0001E32F48" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F48", "uniref90_id": "UniRef90_C4XE27" }, "psc": { "uniref90_id": "UniRef90_C4XE27", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_N9V249" }, "pscc": { "uniref50_id": "UniRef50_N9V249", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V249" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaD" ], "id": "IGIGPGHMMC_191", "locus": "GCA000148625_00152" }, { "type": "cds", "sequence": "contig_1", "start": 83075, "stop": 83419, "strand": "-", "frame": 1, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_A0A3P8KM71", "UniRef:UniRef90_C4XE26" ], "nt": "TTGGGAGAATTGGAAAAAATCATTTATCCTTTAATTTTTGAAGAAATAAAAGATGGAAATTTTTTCATTGTTGAGATACCAAATTTATTGCCAAAAAATTTTAAATTTATTTCTTTGTTTTCTGCAGTTTTGTGCTTAGAAACTAGCCAAAAAAATAGGTTAAAAAATTTTAAAAAACGTAATGTGGATAAAACTAAAATTAAGGCAATAGATGAAAAAAACGACCCTTTTTCGATAAAAAATCAACTTTTTGGGTCTATACCAATTGTGGATTTCTATGGGCATAACTTCACTAATAATGAAAATATTTTAGATTTTTTAAAACTTCTATTTTTTATCTCATAA", "aa": "MGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNDPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS", "aa_hexdigest": "d27a05e031156e772cd0eea87426fa65", "start_type": "TTG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "3-prime", "pseudogene": { "start": 83075, "stop": 83647, "observations": { "mutation": [ 83443 ], "stop": [ 83443 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE26", "query_cov": 1.0, "subject_cov": 0.6, "identity": 0.982, "score": 223.0, "evalue": 6.64e-73, "gene_start": 1, "gene_end": 114, "reference_start": 77, "reference_end": 190, "reference_sequence": "MIALIGQIAVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKLWIDQKAHNLGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNNPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS" }, "paralog": false, "identity": 0.9842105263157894, "score": 375.0, "evalue": 2.68e-135, "description": "Internal stop codon at 83443. Nonsense mutation around 83443.", "psc": { "uniref90_id": "UniRef90_C4XE26", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_A0A3P8KM71", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE26", "EC:2.7.1.24", "UniRef:UniRef50_A0A3P8KM71", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM71" ], "product": "Dephospho-CoA kinase" } }, "id": "IGIGPGHMMC_192", "locus": "GCA000148625_00153" }, { "type": "cds", "sequence": "contig_1", "start": 83441, "stop": 83647, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTAATTGGTCAAATTGGCGTGGGAAAGAGTACTTTTTTAGAAAATTTTAAGAAATTAGGTCTTAAAACTTTTAATTGTGACGAGTTTATTAATCGTGAATATCAAAAAAATGGCAAAATTTACCACAAAATTAATCAAGAATTAGGTGATTTTTTAAATGATAAAAATGGTATAAGTAAGGAAAAAATTAAACTTTGA", "aa": "MIALIGQIGVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKL", "aa_hexdigest": "68a2277d2a8dfa07d0dcdd79d9c55696", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7835.0223, "isoelectric_point": 9.336520957946778 }, "id": "IGIGPGHMMC_193", "locus": "GCA000148625_00154" }, { "type": "cds", "sequence": "contig_1", "start": 83649, "stop": 84023, "strand": "-", "frame": 3, "gene": null, "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354345.1", "SO:0001217", "UniParc:UPI0001E32F4A", "UniRef:UniRef100_C4XE25", "UniRef:UniRef50_D5E655", "UniRef:UniRef90_C4XE25" ], "nt": "ATGGAAAAAGCACTTTTATATGAAATCAAAAAATCAAAGTTTTATTCTTTAGCTTTTAATGTGACTAGTAAGGAACAAATAAAAGAAGTAGAAGCTAATTTAAGAAAAGAATACAAGAAAGCAACTCATATTTGTCATGGTTATAGCTTTTTAAATAATGGAGTGATCAATGCAGGCTTTAGCGATGATGGCGAACCTAATGGTACAGCCGGCAGACCAATTAGAGATCTTTTAATTATGCGCAAAATTAATAATGTTGTTGTTTTTGTAATTCGCTATTTTGGTGGAATTAAGTTGGGAGCCAACGGTTTAGCTAGAGCTTATATTAAAAGTGCAAACTTAGCTATAGAAGAATATATAAAGGAGGGAGCCTAA", "aa": "MEKALLYEIKKSKFYSLAFNVTSKEQIKEVEANLRKEYKKATHICHGYSFLNNGVINAGFSDDGEPNGTAGRPIRDLLIMRKINNVVVFVIRYFGGIKLGANGLARAYIKSANLAIEEYIKEGA", "aa_hexdigest": "28f4398ba41d5b95b867a2e497c60f17", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F4A", "ncbi_nrp_id": "WP_013354345.1", "uniref100_id": "UniRef100_C4XE25", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F4A", "RefSeq:WP_013354345.1", "UniRef:UniRef100_C4XE25" ] }, "ips": { "uniref100_id": "UniRef100_C4XE25", "uniref90_id": "UniRef90_C4XE25" }, "psc": { "uniref90_id": "UniRef90_C4XE25", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D5E655" }, "pscc": { "uniref50_id": "UniRef50_D5E655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E655" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_194", "locus": "GCA000148625_00155" }, { "type": "cds", "sequence": "contig_1", "start": 84090, "stop": 84536, "strand": "-", "frame": 3, "gene": null, "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "RefSeq:WP_013526638.1", "SO:0001217", "UniParc:UPI0001F59BD7", "UniRef:UniRef100_C4XE24", "UniRef:UniRef50_A0A449BAM0", "UniRef:UniRef90_C4XE24" ], "nt": "ATGGCTAAAAAAAATATTATTGCTCTTGCTAGCGACCATGGTGGTTGCGAATTAAAAAATGAAATGCGCGATTATGTAAAAAGTTTAGGTTATGAAGTTGTTGACTTAGGACCTGCGGATGGCTCAAAAAGTATTTCATATGCTGAACAAGGGCATAACTTAGCTAAATATATTGATGAACACAAAAACACAATTGGTTTAGGTTTTTGTGGTACAGGTTTAGGTATTTCATATGCCTTAAACCGTCATAAACACATTCGCGCAGCTAGAGTAGTTACAGTTGAAGATGCAAGGCTTGCTAAATTACATAATAATGCTAATGTTTTAGTTATGGGTGGTAGATATGTACCTTTTGCTGAAGCACAAGCTATGTTTAACGAATACATTAAAACTGAATATGAAGGTGGCAGACACCAAGCAAGAATCGATCAAATCGATGAATTCTAA", "aa": "MAKKNIIALASDHGGCELKNEMRDYVKSLGYEVVDLGPADGSKSISYAEQGHNLAKYIDEHKNTIGLGFCGTGLGISYALNRHKHIRAARVVTVEDARLAKLHNNANVLVMGGRYVPFAEAQAMFNEYIKTEYEGGRHQARIDQIDEF", "aa_hexdigest": "4f5c7f5bd1fa86bfcb99770a47fce7af", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59BD7", "ncbi_nrp_id": "WP_013526638.1", "uniref100_id": "UniRef100_C4XE24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BD7", "RefSeq:WP_013526638.1", "UniRef:UniRef100_C4XE24" ] }, "ips": { "uniref100_id": "UniRef100_C4XE24", "uniref90_id": "UniRef90_C4XE24" }, "psc": { "uniref90_id": "UniRef90_C4XE24", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_A0A449BAM0" }, "pscc": { "uniref50_id": "UniRef50_A0A449BAM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAM0" ], "product": "Ribose-5-phosphate isomerase B" }, "genes": [], "id": "IGIGPGHMMC_195", "locus": "GCA000148625_00156" }, { "type": "cds", "sequence": "contig_1", "start": 84669, "stop": 85295, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAAAAAAACAAGAAGATATAAAAGACTGTGGTTTATATGTTTTGCAGTATTTTGTGGCAAAACATAATGACGACTACATTGATATTAATTATTTTAAAAATAAGGCAACTTACTCTTCAGAAGGAGTAAATATTTCAACACTTAAAAAATTAGCTTCTGAACACGATATCGATTTAAATAGTTATACAGGTGATTTTGAATCTCTTGAATCTTTGGACAATTCAAATATGCCTATAGCTTTATTATTAAATAGAAAAGGATATAGCCATTATGTAATTTTAGAAAAAATGAAGGACAATTATTTTATTGTTCAAGATCCAGCTTATGGTCGCAAGATGAAAGTTAAAAAGAAAGACTTGGCTAAGGAATTTGCCAATGTGATACTATTTTTTGATCCAAAAGAAGAAAAATCAAAAAATAAAAATAAAAACTATTCACTTGATAATAAAATAAGCACATTATTTTCATTTAAAAAATATACTTATCCTTTATTCATTTCAGCACTTTTAGGATTGTTTTTATCATTTGGATCAACATTTTTTATAAAAATAGTTTTTGATTTTATATTGCCTAATTATCTTAAAAATAATTTGATTATTATTTTTATATTTTTCCTATGA", "aa": "MRIKKQEDIKDCGLYVLQYFVAKHNDDYIDINYFKNKATYSSEGVNISTLKKLASEHDIDLNSYTGDFESLESLDNSNMPIALLLNRKGYSHYVILEKMKDNYFIVQDPAYGRKMKVKKKDLAKEFANVILFFDPKEEKSKNKNKNYSLDNKISTLFSFKKYTYPLFISALLGLFLSFGSTFFIKIVFDFILPNYLKNNLIIIFIFFL", "aa_hexdigest": "dcb3408152b0a80b8f939adfac24937e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24392.008400000028, "isoelectric_point": 9.086319160461429 }, "id": "IGIGPGHMMC_196", "locus": "GCA000148625_00157" }, { "type": "cds", "sequence": "contig_1", "start": 85308, "stop": 85559, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAATCGGTTGTTTAAAAATCATGTTATCAAAAAAATAAAAAACGATTTAGAGTTTACTTTAACAAAAGCATTTTTTGACAAAATAAAAAGAGTGCCATCAAATTATTTATCTAAATTAACAAGCAATGATTACTTTAAAAGACTTACTTATATAAAAGAAATATGTGAATTTCAATCTAATTTTGTCTTTACTTTTTTAAGTGAAATATTTTCTTTTATAGGTTCAACAATCTTATTGATTTGA", "aa": "MFINRLFKNHVIKKIKNDLEFTLTKAFFDKIKRVPSNYLSKLTSNDYFKRLTYIKEICEFQSNFVFTFLSEIFSFIGSTILLI", "aa_hexdigest": "ada8443b97f9896a4645d090619f7959", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9958.638099999993, "isoelectric_point": 9.673174858093262 }, "id": "IGIGPGHMMC_197", "locus": "GCA000148625_00158" }, { "type": "cds", "sequence": "contig_1", "start": 85722, "stop": 85835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTAGATTATTCAAGATTTATTTCTAAAAACCAATTTAAAAACATGTTGGAATTTAAGAAAAAAGAATATTCATTTGCTTCTAAAGATGATATTAAAAACTTTTGA", "aa": "MDNVDYSRFISKNQFKNMLEFKKKEYSFASKDDIKNF", "aa_hexdigest": "5e6c384aeaedc959b54a9a48799bcbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4557.1226000000015, "isoelectric_point": 9.100179862976074 }, "id": "IGIGPGHMMC_198", "locus": "GCA000148625_00159" }, { "type": "cds", "sequence": "contig_1", "start": 85926, "stop": 86732, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATGTTCTTAAATGGAATAAATTTCTTTGTTAATCCAATGCTAAATATGAGCTCATTATTACTTTCTCAAACAATGATCAAAAGAAATGTTAATTTAGTAAATTTTGTTTTAAATTTAGACGAAAAAAGCAATTTAGATACTGGTTTATTCATCGATAAAATTAAAAAAATTGAATTAGAAAATGTTAATTTTTATTACGAAAACGGAAAAAATATTTTAAATATTCCTTCATTTACGATTGACCAAAATATTCAATTAAATGGTAAAAATGGTAGTGGTAAAACCACTATGCTAAGTTTTATAAACGGCTGTTTTAAAGGTTATTTAGGAAAAATGAAGATTAATGATATTGATTTTGATTCAATAAATTTGATTAATTATCAAAGTAAAAATATATTGATAAATTCTTCCAGCCATTTACCAGATGTTTCTTTTCTCGAATATATTACAAGTAACAATGAAAATTATAAAAATGAATTGAATAATAATATTCAAAAATATGATCTATACAAAATATTTGAAGAAATGAATTTTAATTTAAATAGCAGCATAATTAGTAATGGCAGCAATTTGTCAAGTGGACAAAAACAACTTTTATTATTACTAAAATTATTTGTAAGAAAATATGACTTAATCATGCTTGATGAAGCCTTTGAAAATATTAATCCAAGTAATTGTGAATTATTGAAAAAGGCTATAAAGAAATATCAAGAGGAAGCATTATTTATTGAAATAAGTCACAATAGGAACTATTTATTTAAAACAAAGGAGGTGTGCTTTGAAAAAATTAACAACTGTTTCTAA", "aa": "MMFLNGINFFVNPMLNMSSLLLSQTMIKRNVNLVNFVLNLDEKSNLDTGLFIDKIKKIELENVNFYYENGKNILNIPSFTIDQNIQLNGKNGSGKTTMLSFINGCFKGYLGKMKINDIDFDSINLINYQSKNILINSSSHLPDVSFLEYITSNNENYKNELNNNIQKYDLYKIFEEMNFNLNSSIISNGSNLSSGQKQLLLLLKLFVRKYDLIMLDEAFENINPSNCELLKKAIKKYQEEALFIEISHNRNYLFKTKEVCFEKINNCF", "aa_hexdigest": "3a0182d10fee4aac0d796f3979712fcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 140, "aa_cov": 0.5149253731343284, "hmm_cov": 0.9, "evalue": 2.8224854324502077e-18, "score": 62.863162994384766, "start": 82, "stop": 219 } ], "seq_stats": { "molecular_weight": 31089.449600000033, "isoelectric_point": 6.746640586853029 }, "id": "IGIGPGHMMC_199", "locus": "GCA000148625_00160" }, { "type": "cds", "sequence": "contig_1", "start": 86755, "stop": 87093, "strand": "+", "frame": 1, "gene": null, "product": "MAG1140 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05", "UniRef:UniRef90_A0AB32XAW0" ], "nt": "TTGTTAGCTATTGCAACAGTAACTTTAATTGTTTTTATTTGTTTATATCATGTTGACAAAACAATAAGAGTTAAGTTACAAGTTAATGACAAAAAAGACTTAACTTTGTTAGCAAATGATAAGACTGCTTATTTATTAAAGAAAAATCAAAAAATAATAATTAATGTAAATGACCAAATTTTCAATTTAAAAATCCGCAACTTAAAAATTGAAAATCAAACGATTAAAGTAACCTTCTATAATTTGCCTAATAGTTTGAAACTTTTACCTAATACAACTTTAGATGCCACGATATTTGTGGATCGAGCCACTCTATTAAATTTAATATTAAATTCATAA", "aa": "MLAIATVTLIVFICLYHVDKTIRVKLQVNDKKDLTLLANDKTAYLLKKNQKIIINVNDQIFNLKIRNLKIENQTIKVTFYNLPNSLKLLPNTTLDATIFVDRATLLNLILNS", "aa_hexdigest": "4928e7b648ebfbc2b2b36daba65916e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XAW0", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.991, "score": 202.0, "evalue": 1.92e-65, "valid": true, "product": "MAG1140 family protein", "uniref50_id": "UniRef50_A0A3P8LI05" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05" ] }, "genes": [], "id": "IGIGPGHMMC_200", "locus": "GCA000148625_00161" }, { "type": "cds", "sequence": "contig_1", "start": 87114, "stop": 87332, "strand": "+", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "GO:0004540", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_L0RUR5", "UniRef:UniRef90_C4XE22" ], "nt": "ATGGTTGAATTAAATGTTGTAAACAAGACTAAAAATAAATTTTTGTTTGAAAAAGAATACAAGCAAATATTAGAAAATTTAGCTAAAGAATTTGGGCTTAATCAAACTGTTTCAGTTGATGTAACTATTACAAATAATGATGAAATCCAAAAACTGAATAAAGAATATAGAAACAAAGATTATCCAACAGATATCTTGTCTTTTGGTTTAGAAGATTGA", "aa": "MVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLED", "aa_hexdigest": "9720586fa9bf70882e50697d09f9bd76", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "3-prime", "pseudogene": { "start": 87048, "stop": 87569, "observations": { "mutation": [ 87330 ], "stop": [ 87330 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE22", "query_cov": 1.0, "subject_cov": 0.4161849710982659, "identity": 1.0, "score": 141.0, "evalue": 2.43e-41, "gene_start": 1, "gene_end": 72, "reference_start": 23, "reference_end": 94, "reference_sequence": "MCGSSHSIKFNIKFIINNIIRIMVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLEDWELFENMPIYPLGELVISHEKVEAQAQEFVHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE" }, "paralog": false, "identity": 0.9826589595375722, "score": 340.0, "evalue": 3.01e-122, "description": "Internal stop codon at 87330. Nonsense mutation around 87330.", "psc": { "uniref90_id": "UniRef90_C4XE22", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_L0RUR5", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE22", "UniRef:UniRef50_L0RUR5", "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUR5" ], "product": "Endoribonuclease YbeY" } }, "id": "IGIGPGHMMC_201", "locus": "GCA000148625_00162" }, { "type": "cds", "sequence": "contig_1", "start": 87348, "stop": 87569, "strand": "+", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "nt": "ATGCCAATTTATCCTTTAGGTGAATTAGTAATAAGCCATGAAAAAGTTGAAGCACAAGCTCAAGAGTTTGGTCATTCAATTCGTAGAGAATATTGTTATTTATTTGCTCATGGATTAATTCACTTGATGGGATATGATCATGAAACAGAAGTCGAAAGAGCTGAAATGAATAAACTTGTTGATTTAATATTTGAACCATTAAAAATAAGTAGAGAGGAATAA", "aa": "MPIYPLGELVISHEKVEAQAQEFGHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE", "aa_hexdigest": "4069d2eaaa4daa1d06dcff116782426f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024C77EC7", "query_cov": 0.8904109589041096, "subject_cov": 1.0, "identity": 0.631, "score": 90.9, "evalue": 9.81e-23, "valid": false, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_UPI0024C77EC7", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024C77EC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "product": "rRNA maturation RNase YbeY" }, "genes": [ "ybeY" ], "id": "IGIGPGHMMC_202", "locus": "GCA000148625_00163" }, { "type": "cds", "sequence": "contig_1", "start": 87843, "stop": 87965, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAATTTATGCCCGATGATGGTTTAATTTACCAATACAATAAAAATGGCGACAAATGTAGAGTAAATAAAGTAATTGAAATGGTCCCATTTCCTATTAGAACAGAGCAAATTAAATAA", "aa": "MTEFMPDDGLIYQYNKNGDKCRVNKVIEMVPFPIRTEQIK", "aa_hexdigest": "2888be777c1f96d3b037da4c5c27f0e2", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4762.5082, "isoelectric_point": 6.058149909973144 }, "id": "IGIGPGHMMC_203", "locus": "GCA000148625_00164" }, { "type": "cds", "sequence": "contig_1", "start": 87965, "stop": 88792, "strand": "+", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q98QI1", "UniRef:UniRef90_C4XE20" ], "nt": "ATGAAAGTATGTTTAGCTAGTATTATCGGCAGACCTAATGTAGGAAAATCATCATTAATAAATCAAATTGTTAAATATGATGTTGCAATTGTAACTAATGTTCCGCAAACAACAAGAGATCAAATTGTCGGCGTTTATAACGAAGATGATTTTCAATTAGTTTTTGTTGATACACCAGGTATCCACAAACCATTAAATTTATTAGGTGAAGCCTTAAATAAAAGTGCTTTTGAATCTACAAAAGATGTTGATTGTTTAATATTTCTTTCTCCTGTTAATGAAAAAGTTGGACAAGGAGATATTTTAATTTTAGATAAGATCAAAAATACCAAGAATAAAATTGCAGTCATTTCTAAAATTGATTTAGCCAAAAAACCAGAAGAAATACAAGAAAAAATTGAAGAATTAAGCAAATACAGTTTTGACAAAATATTGTCTGTTTCAAGAAATAATGAAAACTCAATTGAAGAATTAATTAAAGTTATTAAGCAATATGCCTATGAAGGTGAGCCATTTTATGATGAAGACTATATAACAGATAAATCAATGAGATTTTTAGCAAAAGAGATAATAAGAGAAAGTGCGATTAATCTTTTAAGAGAAGAGTTGCCACACTCAATTGCTGTAGAAGTTATTGATTTTATTGAAGAAGATGATATTAAAATAAATGCCATCATTTATGTCAAAAAAGCTTCTCAAAAAGGAATGGTAATTGGCAAAAATGGTCAAATGATAAAACAAATAGGAACCAACGCAAGAAAGAAAATGATGAGTCAATTTGATTCAAAAGTGGAATTAAATTTAAAAGTTAAAATAGCAAACAAATGA", "aa": "MKVCLASIIGRPNVGKSSLINQIVKYDVAIVTNVPQTTRDQIVGVYNEDDFQLVFVDTPGIHKPLNLLGEALNKSAFESTKDVDCLIFLSPVNEKVGQGDILILDKIKNTKNKIAVISKIDLAKKPEEIQEKIEELSKYSFDKILSVSRNNENSIEELIKVIKQYAYEGEPFYDEDYITDKSMRFLAKEIIRESAINLLREELPHSIAVEVIDFIEEDDIKINAIIYVKKASQKGMVIGKNGQMIKQIGTNARKKMMSQFDSKVELNLKVKIANK", "aa_hexdigest": "fd3af8ade559fc7fb09080e965318b26", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XE20", "query_cov": 1.0, "subject_cov": 0.9482758620689655, "identity": 1.0, "score": 518.0, "evalue": 3.06e-185, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_Q98QI1", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QI1" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "IGIGPGHMMC_204", "locus": "GCA000148625_00165" }, { "type": "cds", "sequence": "contig_1", "start": 89323, "stop": 89916, "strand": "+", "frame": 1, "gene": null, "product": "M24 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "nt": "ATGACAGAAAAAGAAGTAGCTGCATACTTAAATTATTTAATGAAAAAACATGGATCAGATAAAGAAAGTTTTGATGAAATAGTTGCTACAGGGCCTTCATCAGCTGAACCTCACCACCATCCAACAGATAGAAAATTAAAAGAAGGGGATCTTCTTAAAATCGACTTTGGTGCTCTTTACAAAGGATACAGTGCAGATATTACTAGAACATGTATTTTAGGTGGAGACAAAAAAGCGAATGATCCTAAACAATTAGAAATTTTACAAATAGTTATGGAAGCTGCAAAAGCTGGTCGTGATGCAGTAAGACCTGGAATTAAAGCAAGTGAAATTGATAAAATTTGTCGTGATTACATAACTAAAAAAGGATATGGTAAATACTTTGTTCACTCAACTGGTCATGGGCTTGGAATTGATGTACATGAATTGCCTAATGTTAGATCAACAAGTGACTATATTTTAGAACCTGGAATGATAATTACTGTTGAACCTGGTATTTATATTGAAGGTTTAGGTGGAGCAAGAAATGAAGATGATGTTCTTGTTACAGAAAAAGGTCGTTATGTATTCAGTAGACCTGAAGAAAAATCTTAG", "aa": "MTEKEVAAYLNYLMKKHGSDKESFDEIVATGPSSAEPHHHPTDRKLKEGDLLKIDFGALYKGYSADITRTCILGGDKKANDPKQLEILQIVMEAAKAGRDAVRPGIKASEIDKICRDYITKKGYGKYFVHSTGHGLGIDVHELPNVRSTSDYILEPGMIITVEPGIYIEGLGGARNEDDVLVTEKGRYVFSRPEEKS", "aa_hexdigest": "bbf05903b3e9a818f78b4c01a085a7bf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00313C6812", "query_cov": 0.8883248730964467, "subject_cov": 0.8663366336633663, "identity": 0.514, "score": 177.0, "evalue": 4.17e-53, "valid": false, "product": "M24 family metallopeptidase", "uniref50_id": "UniRef50_UPI00313C6812" }, "pscc": { "uniref50_id": "UniRef50_UPI00313C6812", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "product": "M24 family metallopeptidase" }, "genes": [], "id": "IGIGPGHMMC_205", "locus": "GCA000148625_00166" }, { "type": "cds", "sequence": "contig_1", "start": 89990, "stop": 90361, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01938.26" ], "nt": "ATGAAATATGAATTAGGCCAAATAATAAAAGGAGTGGAAGCAATAAATTTTACTTATGAAGCATTAGGCTTAGTTAAAGTAGATGATTATTCTATTTTTGTTGAGAATTTATTGCCACAAGAAAAAGCAGATATACAAATTAAAAAATCGAACACAAAATTTGCTTTTGCTATAGTTGTAAAAAGATATAACGATAGTCCAAAAAGAATAAAAGTTTTAAATCAAAAATTAATGCTAAGTGGTTCAACTCCATTAGCAATTTTAAACTATGCAGATCAATTAGAATTTAAAGAAAATGTTGTTAAATATTTATTTCAAAGAAATATCCATTTTAATGAAACACAAAAAATTTTGCCGTGTGAAAAACAATGA", "aa": "MKYELGQIIKGVEAINFTYEALGLVKVDDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQ", "aa_hexdigest": "353222845af787e8047fd9c04e2314e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01938.26", "name": "TRAM", "length": 45, "aa_cov": 0.34146341463414637, "hmm_cov": 1.0, "evalue": 1.4776428474677156e-07, "score": 27.26605224609375, "start": 19, "stop": 60 } ], "seq_stats": { "molecular_weight": 14272.48169999998, "isoelectric_point": 9.118231010437015 }, "id": "IGIGPGHMMC_206", "locus": "GCA000148625_00167" }, { "type": "cds", "sequence": "contig_1", "start": 90500, "stop": 91306, "strand": "+", "frame": 2, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_C4XE18", "UniRef:UniRef90_C4XE18" ], "nt": "ATGTATTTAAACAAAAATATTAATAATTACAAATGTTTTATAGAAAACGGACAATTTTTAAAACAAATTGTTGTTAGATATTCAGAATCATATGATCAAATGATGTTAGCTTTTGTTCAAGACAAACCATTTGAATTATCTGAAGATTTATTAAAAGATTTAACCTCAAATTTTAAAAATTTAAAAGTTATATTAAAAGAAGAAAAAGTTAAAAAAACAACTTATACTTATTATTCAGAAGCTAAAAACATACAAGACAAAATTAGCAATTTAACTTTTAATATTGATTACAATAGTTTCTTTCAAGTTAACTCGTATCAAACCAATAATTTATATAACTTGCTAGTTGATAATTTGCACCTTGATAAAACTATGAATGTAGTTGATGCATATTCTGGAATTGGAACAATAAGTTTAAAAATTGCAGAAAAAGTTAAAAAAGTTTATGGACTTGAAATAGTAAAAGAAGCAGTTATAAATGCTAAGCAAAATGCAAAATTAAATAACATAAATAATGTAGAATTTTATGAAGGCGATGTTTTAAAAACTATTGATAACATTAAAGAAAAAATAGACACAATAATAATAGATCCTCCAAGAGCAGGAGTATCGAAAGAAGTTTTAGAAAAAATATTAAAAATTGAACCAAAACAAATTGGTTACATAAGCTGTAATCCTCACACACTTTGCAGAGATATAGATTTATTAACATTATCAAAAAAATACAAATTAGAATTTTTAAAACCATGTGATATGTTTTGTCAAACACACCATGTGGAAATAGTTGCAATTTTAAAATTAAAATAA", "aa": "MYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVDNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNVEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVEIVAILKLK", "aa_hexdigest": "5ab8fedd3afab1fad799e8d422a2a46b", "start_type": "ATG", "rbs_motif": null, "genes": [ "rlmD" ], "truncated": "5-prime", "pseudogene": { "start": 90200, "stop": 91306, "observations": { "mutation": [ 90359 ], "stop": [ 90359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE18", "query_cov": 1.0, "subject_cov": 0.6077097505668935, "identity": 0.9890000000000001, "score": 520.0, "evalue": 1.3e-183, "gene_start": 1, "gene_end": 268, "reference_start": 174, "reference_end": 441, "reference_sequence": "MRNMKYELGQIIKGVEAINFTYEALGLVKADDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQWNYRNKITVFVESSKGKIKFGLYEKNTHKLVEQDSYDLANESINKLLMYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVNNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNTEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVETVAILKLK" }, "paralog": false, "identity": 0.9891304347826086, "score": 723.0, "evalue": 7.07e-266, "description": "Internal stop codon at 90359. Nonsense mutation around 90359.", "psc": { "uniref90_id": "UniRef90_C4XE18", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_C4XE18", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE18", "EC:2.1.1.190", "UniRef:UniRef50_C4XE18", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE18" ], "product": "TRAM domain-containing protein" } }, "id": "IGIGPGHMMC_207", "locus": "GCA000148625_00168" }, { "type": "cds", "sequence": "contig_1", "start": 91734, "stop": 92210, "strand": "+", "frame": 3, "gene": null, "product": "DUF3137 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16", "UniRef:UniRef90_C4XE16" ], "nt": "ATGGGAGTTACAATTAAACCAAAGAGTTATTTAGATTTTGTGAATGAAAACTTACAACCATTTAAAGAAATAGTTTCAAACAGTTTTGATTCAGTTATTAAAAATAAAAAGAACATTTTGTCAAAATTAAAAATATTACTAATTGTTTCAATAGTACTATTAGTTTTAGGTGGAATTGGATTAATATTATCTTTCTTTTTCTTCATAAAAACTAATTCAAAAAATACAGCAATATTTGCTGGATTAATTATTTCTGCAATAGTAACAGGAATTGGTTCAATTGTATTATCAGTATTTTTAGGAAAAAGGAAAAAACTTTTTAAAGAACTCAAACACGTTCTTGATAAAGAAAAATTATTTGACAAAGCTTTTGAAAAATTATCATACAAAGCAATCGATCCAAATGACCCAGAACAAGCACAATTATTAGAAAAATTTAAAAAAGGTTATGATAAAAATAGAGATTGTTTAGACTGA", "aa": "MGVTIKPKSYLDFVNENLQPFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKKGYDKNRDCLD", "aa_hexdigest": "b18619d4b491a2e7b5c361c80ed44e80", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 91734, "stop": 92513, "observations": { "mutation": [ 92208, 92235, 92307, 92334 ], "stop": [ 92208, 92235, 92307, 92334 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE16", "query_cov": 1.0, "subject_cov": 0.4328767123287671, "identity": 0.981, "score": 284.0, "evalue": 1.11e-93, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MGVTTKPKSYLDFVNENLQSFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKQGYDKNRDCLDWVDHIPGAYWIPSDANVDSTGKIYFVMDKNENPWVIQTVRYHWVREERDLKGNITIRHIYDYATLFEGIFYSLPKSKNFTLAIGNRCGLTNNLKQNIKYENQEFDKLFKAYCSDSLKGHNIYQPYPMEITLEHCKQNKSYVKSFGMLLDDQNIKCWVRPRGSILEIDMPSNTINKDKIVKTVCKDILEDTYSVYWLFSLLTIPGYFN" }, "paralog": false, "identity": 0.9613899613899614, "score": 486.0, "evalue": 1.16e-175, "description": "Internal stop codon at 92208, 92235, 92307, 92334. Nonsense mutation around 92208, 92235, 92307, 92334.", "psc": { "uniref90_id": "UniRef90_C4XE16", "product": "DUF3137 domain-containing protein", "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE16", "UniRef:UniRef50_C4XE16" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16" ], "product": "DUF3137 domain-containing protein" } }, "id": "IGIGPGHMMC_208", "locus": "GCA000148625_00169" }, { "type": "cds", "sequence": "contig_1", "start": 92851, "stop": 93084, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTTCATTTTATTTTTTAATTTTTTAGTAAAATTTATTTATAAAATTGGAAAGGAATTTATGGCTAAGATTTTTGTACCAAAGACTTTTGCGGAATTTAAAGATACAAATAAACAGTTTATAAATGACAAAATACAATTAATTATAGACAATAAAAATAAAGAAATTAAGACAAGCTTTTTATATTTAATTACATCAATTGTTTTAATATTTCTAGGAATAATTTTTTGA", "aa": "MYFILFFNFLVKFIYKIGKEFMAKIFVPKTFAEFKDTNKQFINDKIQLIIDNKNKEIKTSFLYLITSIVLIFLGIIF", "aa_hexdigest": "0320ef62dc26c55377a5c7d40f2699be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9258.103599999995, "isoelectric_point": 9.60245304107666 }, "id": "IGIGPGHMMC_209", "locus": "GCA000148625_00170" }, { "type": "cds", "sequence": "contig_1", "start": 93208, "stop": 93453, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAAACAAAATTCGATTGTTACAAATATTGACAAAGGTCTAAACGAAGAAGATATTTACAAGCAAATTTTTAACCAAATTAACTATAAAATAGTTGAGTCGGAATATTTAGAAAATGTAAGTGAACAAGAAGAAAAGAATTTTTTATCAATAGAATTAAAGAAAAAAAGTAATATAGAAGAAAGAAAAGAATTGACTAAAAAAATAAATGATATTTTACTTAATGAAGTACCTTTTAACTGA", "aa": "MRKQNSIVTNIDKGLNEEDIYKQIFNQINYKIVESEYLENVSEQEEKNFLSIELKKKSNIEERKELTKKINDILLNEVPFN", "aa_hexdigest": "718784954ef5a7cb8ea629b90ebfb16d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9705.872599999992, "isoelectric_point": 5.032319831848144 }, "id": "IGIGPGHMMC_210", "locus": "GCA000148625_00171" }, { "type": "cds", "sequence": "contig_1", "start": 93835, "stop": 93924, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTTCAGTTAATCACTTTGAACAATATTTTGATAAAAACAAAGATTTTGGATTATTTTTAAAAAGAGATTTAATTAGAGGCTGA", "aa": "MEVSVNHFEQYFDKNKDFGLFLKRDLIRG", "aa_hexdigest": "a0119ad52b43e4144f5ae7d993f6585b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3547.004200000001, "isoelectric_point": 6.52206974029541 }, "id": "IGIGPGHMMC_211", "locus": "GCA000148625_00172" }, { "type": "cds", "sequence": "contig_1", "start": 94088, "stop": 94207, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACATTTAATCAATTTTTATCTCGTTCATATGTTCCTTTGTCTAAACATTTAAACGAACAATTAAATAAAGAAGATTCAAAAAACAAAAAGCTTAGTAAATCTTTCTTATGA", "aa": "MKETFNQFLSRSYVPLSKHLNEQLNKEDSKNKKLSKSFL", "aa_hexdigest": "e97974e3b92c057c38c66fc505ddbb18", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4658.2947, "isoelectric_point": 9.777226829528807 }, "id": "IGIGPGHMMC_212", "locus": "GCA000148625_00173" }, { "type": "cds", "sequence": "contig_1", "start": 94217, "stop": 94501, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTGTGTCTATTAATTATATTGGCTTCAATTTCAATAGTTCTGATGTTGTCATTTTTTGAAAAGTCAGATAAGATTGAAATTTTAAAATGGGTTATTGTTGCAATTTTGATTATAGCAAATTTGTTTTGTGGAATAAGTTTTACATTTTCAAGAAAGGCAGAAGTTATTGAAAAAAGTCGAAATTTGGCAAAAATTAATCTTAATAATTCATTAAATTATTTATTTAAATTACTTAATTTTGAATACAAAAATGAAGAAAAAAATAACAATATTCCATGA", "aa": "MGLCLLIILASISIVLMLSFFEKSDKIEILKWVIVAILIIANLFCGISFTFSRKAEVIEKSRNLAKINLNNSLNYLFKLLNFEYKNEEKNNNIP", "aa_hexdigest": "9712f8cb468c1eabd03fb1fb83e9f894", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10811.826099999986, "isoelectric_point": 9.002445793151857 }, "id": "IGIGPGHMMC_213", "locus": "GCA000148625_00174" }, { "type": "cds", "sequence": "contig_1", "start": 94679, "stop": 94909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAAATTCATATATTTCATGTGGAAGCAAAAATGAAATTTATGATTATTTAAAAAAGAAAAATAAAGTTAAATTTATTCATCAAAATGATCAAATTCATTTCAAAACTTCAAGTAAAGAAACATATGAACTAACTGACACAATTAAGACTTTTGAAGAATTTAAATTGAATGAAAAAGATTATGGATTTTTAATTGATCCAAAAAATAAAGTGTCTTGCAGTTGA", "aa": "MTENSYISCGSKNEIYDYLKKKNKVKFIHQNDQIHFKTSSKETYELTDTIKTFEEFKLNEKDYGFLIDPKNKVSCS", "aa_hexdigest": "bbf2c3e1021dab5c70bb896f1552d79b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9025.079899999993, "isoelectric_point": 7.728363609313964 }, "id": "IGIGPGHMMC_214", "locus": "GCA000148625_00175" }, { "type": "cds", "sequence": "contig_1", "start": 95049, "stop": 95597, "strand": "-", "frame": 3, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "RefSeq:WP_013354357.1", "SO:0001217", "UniParc:UPI0001E32F54", "UniRef:UniRef100_UPI0001E32F54", "UniRef:UniRef50_A0A3P8MDG1", "UniRef:UniRef90_C4XE55" ], "nt": "ATGTTAAGAATTATCAGTGGAAAATATCGTCATTTAAATATAGAACAACCTAAAACTTTGGAAACAAGACCAACTATGGATCGTGTTCGTGAAGCTATTTTTTCATCAATAAGATTTAACTTAGAAAGTGCTTGCATTTTAGATCTTTTTGCAGGTAGTGGTGCTTTTAGTTTTGAAGCATTAAGCAACTATGCGGTAAAAGCAATAGCTGTTGATAATAATAATGAAGCAATTAAAACAATAAGAAATAATGCTTTAAAACTAAAAGCTGAAAATATCGATATTGTAAAAGATGATGTTTTGAATTATTTACAAAAGAATTCGGGACGTAAATTTGATTTTATTTTTATTGATGCTCCATACAAGGAATATCAATCGGTTAATAAATCTCTTGAATTGATAGTTGATAATAAATTTTTAACTAAAAATGGTTTAGTTATTTTAGAAACTGACAATTTCTTAATGATTGATATTCCAAAAAAGCTTACAATACAAAAACAAAGACAATATGGAAAAGTAAATATTTTGTTTATCTCAAATAAAAATTAA", "aa": "MLRIISGKYRHLNIEQPKTLETRPTMDRVREAIFSSIRFNLESACILDLFAGSGAFSFEALSNYAVKAIAVDNNNEAIKTIRNNALKLKAENIDIVKDDVLNYLQKNSGRKFDFIFIDAPYKEYQSVNKSLELIVDNKFLTKNGLVILETDNFLMIDIPKKLTIQKQRQYGKVNILFISNKN", "aa_hexdigest": "9dc0fefb82c882d5f5e693b19266af1c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F54", "ncbi_nrp_id": "WP_013354357.1", "uniref100_id": "UniRef100_UPI0001E32F54", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F54", "RefSeq:WP_013354357.1", "UniRef:UniRef100_UPI0001E32F54" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F54", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "uniref90_id": "UniRef90_C4XE55" }, "psc": { "uniref90_id": "UniRef90_C4XE55", "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A3P8MDG1", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDG1" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "IGIGPGHMMC_215", "locus": "GCA000148625_00176" }, { "type": "cds", "sequence": "contig_1", "start": 95668, "stop": 96237, "strand": "+", "frame": 1, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "RefSeq:WP_013354358.1", "SO:0001217", "UniParc:UPI0001E32F55", "UniRef:UniRef100_UPI0001E32F55", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_C4XE56" ], "nt": "ATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTACATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAATCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAA", "aa": "MKYEIKLVELPEKILRKKSKEVKIPLSKEDEELAKKMIYHIDESQKEDSKLGFRPGVGVAAIQYGIPKRMFYININNETINGAKIEDFRDVLINPKVLAISEYEVSLPGEGCLSVGDNIKNQEGYVYRKKRIVLEAYSYFEKKVKHYDLVDYAAIVAQHEFDHLEGKLFIDRINKKDPFVIKDDSEILE", "aa_hexdigest": "e6968db4fa5ef8e264534fb16d0de725", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F55", "ncbi_nrp_id": "WP_013354358.1", "uniref100_id": "UniRef100_UPI0001E32F55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F55", "RefSeq:WP_013354358.1", "UniRef:UniRef100_UPI0001E32F55" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F55", "uniref90_id": "UniRef90_C4XE56" }, "psc": { "uniref90_id": "UniRef90_C4XE56", "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "IGIGPGHMMC_216", "locus": "GCA000148625_00177" }, { "type": "cds", "sequence": "contig_1", "start": 96327, "stop": 97037, "strand": "+", "frame": 3, "gene": "cvpA", "product": "CvpA family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57", "UniRef:UniRef90_C4XE57" ], "nt": "TTGATAGTCCTTTTGGTTGCAATTGCTATTGGGGCTAAGAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTCTGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGA", "aa": "MIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNSAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLAS", "aa_hexdigest": "91c3aba2a0cf73cb27aa852bf5b278e7", "start_type": "TTG", "rbs_motif": null, "genes": [ "cvpA" ], "truncated": "both", "pseudogene": { "start": 96234, "stop": 97340, "observations": { "mutation": [ 96273, 96297, 97035, 97077, 97206 ], "stop": [ 96273, 96297, 97035, 97077, 97206 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE57", "query_cov": 1.0, "subject_cov": 0.48459958932238195, "identity": 0.996, "score": 439.0, "evalue": 1.41e-151, "gene_start": 1, "gene_end": 236, "reference_start": 32, "reference_end": 267, "reference_sequence": "MINEVFMRTYEDNWKALATNGWCVLISISVFLIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNSAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLASWSDEEIERLPKVIDWLNYCFNDEASFDLLNNSYNYPTGETIKYYFKGVYSNDPSKATWNTKVLKYGNVYYYKKMNFGGPDSWSPATTTYAESLENKFDFKTMFSWNIFKWSQTLRTLSKIDLDSKFEVSDSKVEDFTKIKKIKNKDIQFKLNVENEVVRNKIKSIFYKLISLENEISNTKIDESKVLGFSTDSANKNQINDNTIKRFQLYFDNLLDTIMG" }, "paralog": false, "identity": 0.9836956521739131, "score": 706.0, "evalue": 5.49e-259, "description": "Internal stop codon at 96273, 96297, 97035, 97077, 97206. Nonsense mutation around 96273, 96297, 97035, 97077, 97206.", "psc": { "uniref90_id": "UniRef90_C4XE57", "product": "CvpA family protein", "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE57", "UniRef:UniRef50_C4XE57" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57" ], "product": "CvpA family protein" } }, "id": "IGIGPGHMMC_217", "locus": "GCA000148625_00178" }, { "type": "cds", "sequence": "contig_1", "start": 97539, "stop": 97697, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTTAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAA", "aa": "MISLENEISNTKIDESKVLGFLTDSANKNQINDNTIKRFQLYFDNLLDTIMG", "aa_hexdigest": "6010e7dd24163d5c008708b929fcdc5f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5966.6599, "isoelectric_point": 4.4576208114624025 }, "id": "IGIGPGHMMC_218", "locus": "GCA000148625_00179" }, { "type": "cds", "sequence": "contig_1", "start": 97848, "stop": 97964, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2", "UniRef:UniRef90_UPI0020BDD874" ], "nt": "ATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGA", "aa": "MQKYDESSIQQLKGLEAVRKRPGMYIGSTDVNGLHHLI", "aa_hexdigest": "b449758f9a54df4fdf5a724069df0dba", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 97857, "stop": 97988, "observations": { "mutation": [ 97962 ], "stop": [ 97962 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0020BDD874", "query_cov": 0.9210526315789473, "subject_cov": 0.660377358490566, "identity": 0.8, "score": 60.8, "evalue": 1.33e-11, "gene_start": 4, "gene_end": 38, "reference_start": 11, "reference_end": 45, "reference_sequence": "MEQKQMPENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSI" }, "paralog": false, "identity": 0.813953488372093, "score": 72.0, "evalue": 4.92e-19, "description": "Internal stop codon at 97962. Nonsense mutation around 97962.", "psc": { "uniref90_id": "UniRef90_UPI0020BDD874", "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0020BDD874", "UniRef:UniRef50_A0A829GPU2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "IGIGPGHMMC_219", "locus": "GCA000148625_00180" }, { "type": "cds", "sequence": "contig_1", "start": 98055, "stop": 98351, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "nt": "ATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATCGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGA", "aa": "MDNGRGIPVGKTPSGKSAVELVFTELHAGGKFNEGAYKTSGGLHGVGSSVVNALSTKLIVTVYRDKKIYETIFENGDNIVQRTLEVGNTNKHGTMVEF", "aa_hexdigest": "a1a737656f6194c0eeefed80e0d6070d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920EUL8", "query_cov": 0.8163265306122449, "subject_cov": 0.8333333333333334, "identity": 0.588, "score": 102.0, "evalue": 1.67e-26, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920EUL8" }, "pscc": { "uniref50_id": "UniRef50_A0A920EUL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "IGIGPGHMMC_220", "locus": "GCA000148625_00181" }, { "type": "cds", "sequence": "contig_1", "start": 98487, "stop": 98918, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00204.31" ], "nt": "ATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGA", "aa": "MYEDGLHAFVDFINDSKNALGDIKTFKETKRDIEVECGFQYTDSYNETVLSFVNNVKTKDGGTHEIGLKSALTKTFNEFALEEKALKGKNTFDGDDIREGLTIILSVKIPEKLLEFVSQTKEKLGTPEAKGVVEEIVSKNFKI", "aa_hexdigest": "d3567811b9e0fe67cd93cb1d3264be85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00204.31", "name": "DNA_gyraseB", "length": 142, "aa_cov": 0.965034965034965, "hmm_cov": 0.9859154929577465, "evalue": 3.955465774455243e-46, "score": 152.67640686035156, "start": 5, "stop": 142 } ], "seq_stats": { "molecular_weight": 16044.96759999999, "isoelectric_point": 5.041698265075683 }, "id": "IGIGPGHMMC_221", "locus": "GCA000148625_00182" }, { "type": "cds", "sequence": "contig_1", "start": 99327, "stop": 99491, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGA", "aa": "MTDADTDGAHIQILLLTFFFRHMRQLVENGMVYIALPPLFKLTSTRGKKEVLYA", "aa_hexdigest": "278818c68beb375cd535309d5b841535", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6212.268, "isoelectric_point": 8.18040943145752 }, "id": "IGIGPGHMMC_222", "locus": "GCA000148625_00183" }, { "type": "cds", "sequence": "contig_1", "start": 99603, "stop": 99713, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGA", "aa": "MNPITRTLVQVKIEDAALAERRVSTLMGDNVEPRKE", "aa_hexdigest": "8cc26119281ffd9c9602e0f30e1db464", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4081.676299999999, "isoelectric_point": 6.16506404876709 }, "id": "IGIGPGHMMC_223", "locus": "GCA000148625_00184" }, { "type": "cds", "sequence": "contig_1", "start": 99774, "stop": 99974, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGA", "aa": "MNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQ", "aa_hexdigest": "488e542d0a74fbc41414112f39c48729", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 24, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 2.1637170098401663e-12, "score": 42.56110763549805, "start": 40, "stop": 63 } ], "seq_stats": { "molecular_weight": 7887.083800000003, "isoelectric_point": 9.62727336883545 }, "id": "IGIGPGHMMC_224", "locus": "GCA000148625_00185" }, { "type": "cds", "sequence": "contig_1", "start": 100089, "stop": 101519, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2", "UniRef:UniRef90_C4XE59" ], "nt": "ATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAACAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGA", "aa": "MGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDNKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESK", "aa_hexdigest": "9c5f5778615b5d8b3c7ede33755da640", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 99789, "stop": 101822, "observations": { "mutation": [ 99972, 100083, 101517 ], "stop": [ 99972, 100083, 101517 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE59", "query_cov": 1.0, "subject_cov": 0.5554259043173863, "identity": 0.998, "score": 921.0, "evalue": 0.0, "gene_start": 1, "gene_end": 476, "reference_start": 107, "reference_end": 582, "reference_sequence": "MMNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQWNKPFKKSARVVGDVIGKYHPHGDSSIYEAMVRMAQDWKMGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDKKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESKWKDFGVHLSDFVELVSGEELVGAINVTDFNIQNYVCLFTKEAQGKRVVLKEFEVSRPNKTFTAMKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK" }, "paralog": false, "identity": 0.9940915805022157, "score": 1314.0, "evalue": 0.0, "description": "Internal stop codon at 99972, 100083, 101517. Nonsense mutation around 99972, 100083, 101517.", "psc": { "uniref90_id": "UniRef90_C4XE59", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE59", "UniRef:UniRef50_I5D5N2" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "IGIGPGHMMC_225", "locus": "GCA000148625_00186" }, { "type": "cds", "sequence": "contig_1", "start": 101706, "stop": 102344, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAA", "aa": "MKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK", "aa_hexdigest": "db991e1d924fe66d1fc9ba1eb9d19c86", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23818.17920000002, "isoelectric_point": 8.704215049743652 }, "id": "IGIGPGHMMC_226", "locus": "GCA000148625_00187" }, { "type": "cds", "sequence": "contig_1", "start": 102443, "stop": 102718, "strand": "+", "frame": 2, "gene": null, "product": "Phosphoesterase", "db_xrefs": [ "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_C4XE60", "UniRef:UniRef90_C4XE60" ], "nt": "ATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGA", "aa": "MEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDY", "aa_hexdigest": "afec43428a0c5d56c07ac79c75d19c96", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 102401, "stop": 102967, "observations": { "mutation": [ 102410, 102716, 102869 ], "stop": [ 102410, 102716, 102869 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE60", "query_cov": 1.0, "subject_cov": 0.4789473684210526, "identity": 1.0, "score": 194.0, "evalue": 6.67e-62, "gene_start": 1, "gene_end": 91, "reference_start": 17, "reference_end": 107, "reference_sequence": "MWIIFWFYFIILKEKIMEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDYWSLRTPNVLFDETKNIDCDFLICGHSHFPIYYKDVKTHKILLNPGSPSLPRWNSKPSYAIITIKNEKKYENVEVIFKEVNEKS" }, "paralog": false, "identity": 0.9840425531914894, "score": 384.0, "evalue": 7.09e-139, "description": "Internal stop codon at 102410, 102716, 102869. Nonsense mutation around 102410, 102716, 102869.", "psc": { "uniref90_id": "UniRef90_C4XE60", "product": "Phosphoesterase", "uniref50_id": "UniRef50_C4XE60", "kegg_orthology_id": "K07095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE60", "UniRef:UniRef50_C4XE60", "KEGG:K07095" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE60" ], "product": "Phosphoesterase" } }, "id": "IGIGPGHMMC_227", "locus": "GCA000148625_00188" }, { "type": "cds", "sequence": "contig_1", "start": 103039, "stop": 103203, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGA", "aa": "MSNKKNKPQYLFAIDLDGTLLASSATGEVHNTTAEAIARAKKEGHIVCALTGRP", "aa_hexdigest": "c214cadee41183746f14a728cbb58d16", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7962962962962963, "hmm_cov": 0.9534883720930233, "evalue": 3.325056565659222e-10, "score": 36.10066223144531, "start": 12, "stop": 54 } ], "seq_stats": { "molecular_weight": 5739.4995, "isoelectric_point": 8.979430580139162 }, "id": "IGIGPGHMMC_228", "locus": "GCA000148625_00189" }, { "type": "cds", "sequence": "contig_1", "start": 103753, "stop": 103971, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTGGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAA", "aa": "MFKVANISVAVGNAPDAVKKYATIKLKKTNRQGAVGEYINKFLDNPEKEIEKSNKVMAKLIKRRDEEADLED", "aa_hexdigest": "0a12320d2db7049ce71bfbd74952a03b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 39, "aa_cov": 0.5416666666666666, "hmm_cov": 0.9743589743589743, "evalue": 1.662043812343905e-07, "score": 27.257429122924805, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 8123.280099999999, "isoelectric_point": 9.222154045104979 }, "id": "IGIGPGHMMC_229", "locus": "GCA000148625_00190" }, { "type": "cds", "sequence": "contig_1", "start": 103974, "stop": 104405, "strand": "+", "frame": 3, "gene": null, "product": "Ribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7", "UniRef:UniRef90_C4XE62" ], "nt": "ATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGA", "aa": "MKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNN", "aa_hexdigest": "90c5062305b19d17c4a57a36634ae7b4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 103971, "stop": 104708, "observations": { "mutation": [ 104403 ], "stop": [ 104403 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE62", "query_cov": 1.0, "subject_cov": 0.572, "identity": 1.0, "score": 285.0, "evalue": 7.11e-96, "gene_start": 1, "gene_end": 143, "reference_start": 2, "reference_end": 144, "reference_sequence": "MMKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNNWADFNQIQGDILLAYKAESISLEVACASIIARDYFLYFMQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKNFKMDLEDQNTTTINLFATDFENKK" }, "paralog": false, "identity": 0.9877551020408163, "score": 489.0, "evalue": 1.23e-178, "description": "Internal stop codon at 104403. Nonsense mutation around 104403.", "psc": { "uniref90_id": "UniRef90_C4XE62", "product": "Ribonuclease", "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE62", "UniRef:UniRef50_A0A6M4JBT7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7" ], "product": "Ribonuclease" } }, "id": "IGIGPGHMMC_230", "locus": "GCA000148625_00191" }, { "type": "cds", "sequence": "contig_1", "start": 104517, "stop": 104723, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAGTTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAA", "aa": "MQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKSFKMDLEDQNTTTINLFATDFENKK", "aa_hexdigest": "182dd0e17efbd0fa4e7af0d630d1a865", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8019.967899999997, "isoelectric_point": 8.599067115783694 }, "id": "IGIGPGHMMC_231", "locus": "GCA000148625_00192" }, { "type": "cds", "sequence": "contig_1", "start": 104993, "stop": 105604, "strand": "+", "frame": 2, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "nt": "ATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAATTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAAAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGATGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGA", "aa": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEKAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDD", "aa_hexdigest": "1c4064bcd07e3e7fa96728bdaa5d15b0", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A7Y0YRM2", "query_cov": 0.9753694581280788, "subject_cov": 0.8181818181818182, "identity": 0.7020000000000001, "score": 273.0, "evalue": 4.43e-90, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_W7C0L0" }, "pscc": { "uniref50_id": "UniRef50_W7C0L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "IGIGPGHMMC_232", "locus": "GCA000148625_00193" }, { "type": "cds", "sequence": "contig_1", "start": 105677, "stop": 106750, "strand": "+", "frame": 2, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "GO:0005524", "GO:0006457", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_C4XE63" ], "nt": "ATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGTGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAG", "aa": "MAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE", "aa_hexdigest": "f932c8163746fec5f053c5d8492b0d77", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PNF8", "query_cov": 0.8991596638655462, "subject_cov": 0.9250720461095101, "identity": 0.579, "score": 346.0, "evalue": 4.94e-115, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_A0A8S2D270" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 105377, "stop": 106750, "observations": { "mutation": [ 105602, 105623 ], "stop": [ 105602, 105623 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE63", "query_cov": 1.0, "subject_cov": 0.6102564102564103, "identity": 1.0, "score": 645.0, "evalue": 2.81e-229, "gene_start": 1, "gene_end": 357, "reference_start": 229, "reference_end": 585, "reference_sequence": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEKAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDDWDHEIVDWLVAKIKNDHKIDIRENKMAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE" }, "paralog": false, "identity": 0.9956236323851203, "score": 861.0, "evalue": 1.11e-316, "description": "Internal stop codon at 105602, 105623. Nonsense mutation around 105602, 105623.", "psc": { "uniref90_id": "UniRef90_C4XE63", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE63", "UniRef:UniRef50_Q49539", "GO:0005524", "GO:0006457", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "IGIGPGHMMC_233", "locus": "GCA000148625_00194" }, { "type": "cds", "sequence": "contig_1", "start": 106939, "stop": 108153, "strand": "+", "frame": 1, "gene": null, "product": "AAA+ ATPase domain-containing protein", "db_xrefs": [ "KEGG:K07478", "RefSeq:WP_013354366.1", "SO:0001217", "UniParc:UPI0001E32F5C", "UniRef:UniRef100_C4XE64", "UniRef:UniRef50_A0A449A4D0", "UniRef:UniRef90_C4XE64" ], "nt": "ATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATCAGCATTTCATAAATCATTAAGAGGAAGTGATGTTGATGCAGCACTTTATTATGGAGCTTTAATCTTAAAAACAGGCGACTACATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAA", "aa": "MKNLANEIRPRTLDDIVGQKHVVSLLKEIVKNKASTSFIFFGESGTGKSSAAVALANDLKLKYGYFNATINNKDELVNTIQTNDIIIIDEIHRLNKDKQDILLSYLEYDKIIVYATTTENPYFKVNPALRSRMQILQFNKLSEEEILFGLKKIIKTYYPKFKIKDDILKVLIKNSTGDYRSCINNLQILTLLNKDKEVTEESIKTLIPNVNFYSDKESNAHYNNLSAFHKSLRGSDVDAALYYGALILKTGDYMGLFRRLTAATYEDVGLADPTLPMRVEAAFNAVERLGFPEAYLPLYYIIISVAIAPKSNTVYKAIQKVEPFIEQGNIYEIPKHLRDAHYKSASKLGDGLDYKYPHDFPNHWVKQEYLPKRIASKKFFEPDNNDSPSLLKYYNFIKNIKGDK", "aa_hexdigest": "8c9719697d297e25499b5530e32b7687", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F5C", "ncbi_nrp_id": "WP_013354366.1", "uniref100_id": "UniRef100_C4XE64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F5C", "RefSeq:WP_013354366.1", "UniRef:UniRef100_C4XE64" ] }, "ips": { "uniref100_id": "UniRef100_C4XE64", "uniref90_id": "UniRef90_C4XE64" }, "psc": { "uniref90_id": "UniRef90_C4XE64", "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_A0A449A4D0", "kegg_orthology_id": "K07478" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4D0" ], "product": "ATPase" }, "genes": [], "id": "IGIGPGHMMC_234", "locus": "GCA000148625_00195" }, { "type": "cds", "sequence": "contig_1", "start": 108581, "stop": 108802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGTAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCGACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGA", "aa": "MHDSLYLNPTTLLRTHNTGISALELERNVNKEMNNFAIGKVYRNDEDDATHSHQFTQLDFVSVGKSVSFNNLI", "aa_hexdigest": "a660cc66fa1783a9801ed2bf87d242d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 79, "aa_cov": 0.9863013698630136, "hmm_cov": 1.0, "evalue": 9.890964320981138e-21, "score": 70.31529998779297, "start": 1, "stop": 72 } ], "seq_stats": { "molecular_weight": 8323.1335, "isoelectric_point": 5.667268180847168 }, "id": "IGIGPGHMMC_235", "locus": "GCA000148625_00196" }, { "type": "cds", "sequence": "contig_1", "start": 108944, "stop": 109117, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATATAACATCAAGGATGTTAGAGAATTTTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAG", "aa": "MLHPNVMELAGYDSKEFNGFAAGIGIERLTMIKYNIKDVREFYKNDLRTLNQFNYEE", "aa_hexdigest": "e6961cf0370b84c54345c6dea50dd53e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 53, "aa_cov": 0.9298245614035088, "hmm_cov": 0.9811320754716981, "evalue": 1.417878401140318e-19, "score": 66.52803802490234, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 6748.5875, "isoelectric_point": 5.2735445022583 }, "id": "IGIGPGHMMC_236", "locus": "GCA000148625_00197" }, { "type": "cds", "sequence": "contig_1", "start": 109107, "stop": 109433, "strand": "+", "frame": 3, "gene": null, "product": "uracil-DNA glycosylase", "db_xrefs": [ "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_N9V498", "UniRef:UniRef90_C4XE67" ], "nt": "ATGAAGAATAGCTTCCAAATATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCCTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGA", "aa": "MKNSFQIFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDPYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKS", "aa_hexdigest": "804beaecb836d24eb14aace213d02f8c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 109095, "stop": 109736, "observations": { "mutation": [ 109431, 109512, 109581 ], "stop": [ 109431, 109512, 109581 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE67", "query_cov": 1.0, "subject_cov": 0.47577092511013214, "identity": 0.991, "score": 212.0, "evalue": 6.3e-68, "gene_start": 1, "gene_end": 108, "reference_start": 5, "reference_end": 112, "reference_sequence": "MNLTMKNSFQTFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDPYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKSWAKQGVLLINTCLSVNENQPLSHKNFGWEIFVINLIKYILKKNKNVIFGIWGNDAKRILQIVQNDYKIDENKIIFTSHPSPLAYSKTTNSFKDSKFFIRVNSLLKNPINWDLRKD" }, "paralog": false, "identity": 0.9765258215962441, "score": 413.0, "evalue": 9.68e-150, "description": "Internal stop codon at 109431, 109512, 109581. Nonsense mutation around 109431, 109512, 109581.", "psc": { "uniref90_id": "UniRef90_C4XE67", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_N9V498", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE67", "EC:3.2.2.27", "UniRef:UniRef50_N9V498", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_N9V498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V498" ], "product": "Uracil-DNA glycosylase" } }, "id": "IGIGPGHMMC_237", "locus": "GCA000148625_00198" }, { "type": "cds", "sequence": "contig_1", "start": 109780, "stop": 110358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATCAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAAAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGTGACTTTGTATTTCAAGATAAAGAAATGGCAGGTGTTGTTTCTCAAGGCATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGA", "aa": "MIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFK", "aa_hexdigest": "0ebbddf26e49b7727938c6bf77eb5f09", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 84, "aa_cov": 0.40625, "hmm_cov": 0.9047619047619048, "evalue": 3.9214501364195594e-08, "score": 29.353538513183594, "start": 44, "stop": 121 } ], "seq_stats": { "molecular_weight": 21730.671700000006, "isoelectric_point": 6.372071647644043 }, "id": "IGIGPGHMMC_238", "locus": "GCA000148625_00199" }, { "type": "cds", "sequence": "contig_1", "start": 110725, "stop": 111657, "strand": "+", "frame": 1, "gene": null, "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A3P8L797", "UniRef:UniRef90_C4XE68" ], "nt": "ATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGA", "aa": "MGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIG", "aa_hexdigest": "24897d433d68fab6222ca4e15ece78af", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 110425, "stop": 111960, "observations": { "mutation": [ 110527, 111655, 111697 ], "stop": [ 110527, 111655, 111697 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE68", "query_cov": 1.0, "subject_cov": 0.4246575342465753, "identity": 1.0, "score": 612.0, "evalue": 4.72e-215, "gene_start": 1, "gene_end": 310, "reference_start": 318, "reference_end": 627, "reference_sequence": "MNMIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFKWFDYKKNYKTGIHKQIQKVDNGEANALSFLECRFKHKDTKIEDMLFLAKHNVEAKNVWAIDVTNLNLIYTGAPTHVYDRSEIGEELTCKKYSGKVTILGNKEIEVKNALAIYSKDKVVSLASVMGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIGWLGKVNPSYNSVDAWVCEVHKEVNKGYKLFENYDSTPFKSIDLTFELKPHQSIDDKIIEIKKVAFIFEIYQIDEFAKPESRNVTLRIIGSNEEIQKINNEFNN" }, "paralog": false, "identity": 0.9882583170254403, "score": 996.0, "evalue": 0.0, "description": "Internal stop codon at 110527, 111655, 111697. Nonsense mutation around 110527, 111655, 111697.", "psc": { "uniref90_id": "UniRef90_C4XE68", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A3P8L797", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE68", "EC:6.1.1.20", "UniRef:UniRef50_A0A3P8L797", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L797", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L797" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "IGIGPGHMMC_239", "locus": "GCA000148625_00200" }, { "type": "cds", "sequence": "contig_1", "start": 111975, "stop": 112514, "strand": "+", "frame": 3, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "EC:2.1.2.1", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P56089", "UniRef:UniRef90_C4XE69" ], "nt": "ATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCAGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAGCCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGA", "aa": "MYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIID", "aa_hexdigest": "d9f4a5bd7785b51daa98e8967244b4ad", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002618815A", "query_cov": 0.9776536312849162, "subject_cov": 0.8293838862559242, "identity": 0.5770000000000001, "score": 197.0, "evalue": 3.74e-61, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A533YJM1", "kegg_orthology_id": "K00600" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 111963, "stop": 112817, "observations": { "mutation": [ 112512, 112722 ], "stop": [ 112512, 112722 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE69", "query_cov": 1.0, "subject_cov": 0.4221698113207547, "identity": 1.0, "score": 345.0, "evalue": 1.87e-116, "gene_start": 1, "gene_end": 179, "reference_start": 5, "reference_end": 183, "reference_sequence": "MGGIMYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIIDWAKFREIADACGAYLMADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDRWVFPGYQGGPLFHTIAGKAVAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTKWRELADIIDTILKNHETILKPESEKLFNSLKKKVRDFTKKFPIITVYR" }, "paralog": false, "identity": 0.9894366197183099, "score": 554.0, "evalue": 5.25e-201, "description": "Internal stop codon at 112512, 112722. Nonsense mutation around 112512, 112722.", "psc": { "uniref90_id": "UniRef90_C4XE69", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "kegg_orthology_id": "K00600", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE69", "EC:2.1.2.1", "UniRef:UniRef50_P56089", "KEGG:K00600" ] }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" } }, "id": "IGIGPGHMMC_240", "locus": "GCA000148625_00201" }, { "type": "cds", "sequence": "contig_1", "start": 112557, "stop": 112724, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTACGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGA", "aa": "MADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDR", "aa_hexdigest": "345e31943a1700467e5aca3128ae9edf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 46, "aa_cov": 0.8363636363636363, "hmm_cov": 1.0, "evalue": 2.272054697711628e-22, "score": 75.16732025146484, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 5839.704599999999, "isoelectric_point": 8.247972297668458 }, "id": "IGIGPGHMMC_241", "locus": "GCA000148625_00202" }, { "type": "cds", "sequence": "contig_1", "start": 112779, "stop": 113093, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGA", "aa": "MAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTK", "aa_hexdigest": "11dd0db7be7e1ddd133574db06e7805b", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 102, "aa_cov": 0.9807692307692307, "hmm_cov": 0.9803921568627451, "evalue": 2.6560019120755105e-34, "score": 114.4564437866211, "start": 2, "stop": 103 } ], "seq_stats": { "molecular_weight": 11428.000799999985, "isoelectric_point": 9.435479927062985 }, "id": "IGIGPGHMMC_242", "locus": "GCA000148625_00203" }, { "type": "cds", "sequence": "contig_1", "start": 113513, "stop": 113770, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGA", "aa": "MNAATIIQLAIYLLPNLLKIPMAIPNILGIITAKVIKFSIIEAIIDLPISFVPWKTRSELIDKLKAITLSVTILINKVVPAALSG", "aa_hexdigest": "38101b8e94f8a3d0d2697a829e0a3953", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.25609999999, "isoelectric_point": 9.777162361145017 }, "id": "IGIGPGHMMC_243", "locus": "GCA000148625_00204" }, { "type": "cds", "sequence": "contig_1", "start": 113860, "stop": 114120, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGCCTACTTTTGCCTTAGGTTTTAGTGGTTTCATAAGATCATTTGGTGTTGCTATTTCATTTGCTGTTATGAACATTTTCTTAACCAAAACCCCTTTTGCAGAACCACTTTCATTCCAATATTATTGGTCAAAAGGTATGCCGATTGTTTCATTATTCTTAGTTTCTATTTTTGGTATTAATGATGGAGCTAGAAGCTTAATGTCATATAACTATACGCGAAGAAACTTTAAAAGATGCAAAGAAGTTTATCTTTGA", "aa": "MLPTFALGFSGFIRSFGVAISFAVMNIFLTKTPFAEPLSFQYYWSKGMPIVSLFLVSIFGINDGARSLMSYNYTRRNFKRCKEVYL", "aa_hexdigest": "9d5d739d0015717808804b47359bbafe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9872.533399999991, "isoelectric_point": 9.916414070129392 }, "id": "IGIGPGHMMC_244", "locus": "GCA000148625_00205" }, { "type": "cds", "sequence": "contig_1", "start": 114166, "stop": 114270, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTATTAGGTGGTGTTGTTGCTACTATTATTGGTTGGTCAATTAACTTAACTGCCTACTTAGCTTATGTGGTAATTAATAGCAAGCGGAATAAAACCTGA", "aa": "MGVLGGVVATIIGWSINLTAYLAYVVINSKRNKT", "aa_hexdigest": "3d1d62a2394cb888ef13294ae4f8eb2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3623.2691000000004, "isoelectric_point": 9.996161460876465 }, "id": "IGIGPGHMMC_245", "locus": "GCA000148625_00206" }, { "type": "cds", "sequence": "contig_1", "start": 114634, "stop": 114900, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCAATTTTTACCAGATACTTTAAACTCTTTAACTTCAAACGAATTGAAGCAGAAATACTTGGATATCATAAATAACTCCAGTTATAAAGATCTGAATTTAGGAAAAATTGATGCTAATGGGGTTGTTTCAATGACCACAGCTGCAATTCAACCTTTAATTATTTTTTCTAACTCAATAGTATTTTTAGTACCTGTTGGTGCAAGTATTTATTACACAAAATGTATTTCTAAAAAATTAGAAAAAAGCGGAAGAGACTTGTGA", "aa": "MIQFLPDTLNSLTSNELKQKYLDIINNSSYKDLNLGKIDANGVVSMTTAAIQPLIIFSNSIVFLVPVGASIYYTKCISKKLEKSGRDL", "aa_hexdigest": "203bcc40dc06ae7e616f1f5464935e5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9728.218799999995, "isoelectric_point": 8.759271049499514 }, "id": "IGIGPGHMMC_246", "locus": "GCA000148625_00207" }, { "type": "tRNA", "sequence": "contig_1", "start": 115182, "stop": 115257, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 75.0, "nt": "GCCGTCATAGCTCAGTCGGTAGAGCACATCCATGGTAAGGATGGGGTCGCTGGTTCAAGTCCAGTTGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 115238, 115240 ], "id": "IGIGPGHMMC_34", "locus": "GCA000148625_00208" }, { "type": "cds", "sequence": "contig_1", "start": 115507, "stop": 116628, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "RefSeq:WP_013354372.1", "SO:0001217", "UniParc:UPI0001E32F81", "UniRef:UniRef100_UPI0001E32F81", "UniRef:UniRef50_C4XE71", "UniRef:UniRef90_C4XE71" ], "nt": "ATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAACTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTCAGCAAGTTTACTCAAAACATTTTTGAAACAGTATTCAATTTTGGAGGCGGAACCTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGTGATGATATTTACACTGAAATCGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTCGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATATTAGAACTTGTAATGTTTGTAAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAA", "aa": "MPKKNYYDVLGVPKTASEREIKTAYRKLAMKYHPDKLKDGTSDQKMQELNEAYEVLSDPTKKSNYDRFGSPDGPRPGQGFGMNFGGEMRDFSKFTQNIFETVFNFGGGTSKQKSSKVEKARGDDIYTEIVVDFIEAVKGKEFKEKLNKFELCSRCKGTGADNPSDIRTCNVCKGKGQKETQTRSVLGYMKMVSTCDKCNGTGKLIGKTCRECNGSLYTKKLKNVTFKIPEGSDSGDKIKIDGYGEKGRNGGESGDLYIVIIVKEHKYFKRNGLDLFLELPVSFLDIVKENSVLVPTPYGFERIKMKNTYQFGKVLNLTGKGIRTKKGTGNLKVALQVVMPNFTSDQYNEMAKILEPYEDNVNAEFSELVKSEK", "aa_hexdigest": "ededb739b4dc8dc166cc85e0f0b26b70", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F81", "ncbi_nrp_id": "WP_013354372.1", "uniref100_id": "UniRef100_UPI0001E32F81", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F81", "RefSeq:WP_013354372.1", "UniRef:UniRef100_UPI0001E32F81" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F81", "uniref90_id": "UniRef90_C4XE71" }, "psc": { "uniref90_id": "UniRef90_C4XE71", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_C4XE71" }, "pscc": { "uniref50_id": "UniRef50_C4XE71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE71" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "IGIGPGHMMC_247", "locus": "GCA000148625_00209" }, { "type": "cds", "sequence": "contig_1", "start": 116665, "stop": 116901, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72", "UniRef:UniRef90_C4XE72" ], "nt": "ATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGA", "aa": "MKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKK", "aa_hexdigest": "7eb248a538f98237d26455d7d21a1f52", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 116662, "stop": 117141, "observations": { "mutation": [ 116899 ], "stop": [ 116899 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE72", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 1.0, "score": 152.0, "evalue": 8.01e-46, "gene_start": 1, "gene_end": 78, "reference_start": 2, "reference_end": 79, "reference_sequence": "MMKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKKWIDLKNKVDSYDTQNFDLSNNFFKKMSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL" }, "paralog": false, "identity": 0.9874213836477987, "score": 315.0, "evalue": 1.28e-112, "description": "Internal stop codon at 116899. Nonsense mutation around 116899.", "psc": { "uniref90_id": "UniRef90_C4XE72", "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE72", "UniRef:UniRef50_C4XE72" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9479.60649999999, "isoelectric_point": 5.366135215759278 }, "id": "IGIGPGHMMC_248", "locus": "GCA000148625_00210" }, { "type": "cds", "sequence": "contig_1", "start": 116974, "stop": 117141, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAA", "aa": "MSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL", "aa_hexdigest": "4d58b6faf28baa5f09a76ea62af54572", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6282.857500000002, "isoelectric_point": 4.286308097839356 }, "id": "IGIGPGHMMC_249", "locus": "GCA000148625_00211" }, { "type": "cds", "sequence": "contig_1", "start": 117260, "stop": 117406, "strand": "+", "frame": 2, "gene": null, "product": "4'-phosphopantetheinyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8", "UniRef:UniRef90_C4XE73" ], "nt": "ATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGA", "aa": "MIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAI", "aa_hexdigest": "d87b1d34bf5650a801bdbba8e7e3782f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 117248, "stop": 117568, "observations": { "mutation": [ 117404, 117488 ], "stop": [ 117404, 117488 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE73", "query_cov": 1.0, "subject_cov": 0.4528301886792453, "identity": 1.0, "score": 95.9, "evalue": 1.1e-24, "gene_start": 1, "gene_end": 48, "reference_start": 5, "reference_end": 52, "reference_sequence": "MIRTMIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAIWAIKEAMFKVDNSLFEFKNIKLQKENESWQHKNFDISISHEDDYLVAVVLKKKE" }, "paralog": false, "identity": 0.9716981132075472, "score": 199.0, "evalue": 4.12e-68, "description": "Internal stop codon at 117404, 117488. Nonsense mutation around 117404, 117488.", "psc": { "uniref90_id": "UniRef90_C4XE73", "product": "4'-phosphopantetheinyl transferase domain-containing protein", "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE73", "UniRef:UniRef50_D5E5U8" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "IGIGPGHMMC_250", "locus": "GCA000148625_00212" }, { "type": "cds", "sequence": "contig_1", "start": 117789, "stop": 118187, "strand": "+", "frame": 3, "gene": null, "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74", "UniRef:UniRef90_C4XE74" ], "nt": "TTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGA", "aa": "MIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNR", "aa_hexdigest": "23c9f92da5bd43a2c14388764de830ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 117543, "stop": 118316, "observations": { "mutation": [ 117552, 117603, 117609, 117615, 117696, 118185 ], "stop": [ 117552, 117603, 117609, 117615, 117696, 118185 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE74", "query_cov": 1.0, "subject_cov": 0.5136186770428015, "identity": 0.992, "score": 254.0, "evalue": 1.2e-83, "gene_start": 1, "gene_end": 132, "reference_start": 83, "reference_end": 214, "reference_sequence": "MWFWKRRSNMNYHLKMFLTWWIWLWRIYRISVYARKYRHTPDYYTPQQRYDWLLKKVKKYLKLYNIKVTVEGYDNLPKAPCLLIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNRWKKQEFKVKFLNPIKPSTFMSMDPGALADRVKSLMERELENES" }, "paralog": false, "identity": 0.9727626459143969, "score": 491.0, "evalue": 1.26e-179, "description": "Internal stop codon at 117552, 117603, 117609, 117615, 117696, 118185. Nonsense mutation around 117552, 117603, 117609, 117615, 117696, 118185.", "psc": { "uniref90_id": "UniRef90_C4XE74", "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE74", "UniRef:UniRef50_C4XE74" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74" ], "product": "Phospholipid/glycerol acyltransferase domain-containing protein" } }, "id": "IGIGPGHMMC_251", "locus": "GCA000148625_00213" }, { "type": "cds", "sequence": "contig_1", "start": 118306, "stop": 119064, "strand": "+", "frame": 1, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "RefSeq:WP_013354376.1", "SO:0001217", "UniParc:UPI0001A8C090", "UniRef:UniRef100_C4XE75", "UniRef:UniRef50_A0A449B1R9", "UniRef:UniRef90_C4XE75" ], "nt": "ATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGACATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAA", "aa": "MKASLYATDDHKYDIKLENFDGPLDLLLALVQEKNVDIMDVDVSELATAYLKIIQNLQEHEIDIAGDYLVMAATLLALKTKMLLFTPEEKPEIEEDKREILRRLYEYQQFKEISQTLRQQEALRHEIFIKSPSDVEEFMVDDDSTALDGASNPLKLITTLRKMFERTYAMQLRRTKLETFNLTPKDQIPFILNLFDHCDTVTFEMIFKQPSLNHFVITLLAVLDLSRRQIIILKQNEQFGTITFEKGPEYEK", "aa_hexdigest": "74a996ae10002adb3c97e3bb0b11aa93", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C090", "ncbi_nrp_id": "WP_013354376.1", "uniref100_id": "UniRef100_C4XE75", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C090", "RefSeq:WP_013354376.1", "UniRef:UniRef100_C4XE75" ] }, "ips": { "uniref100_id": "UniRef100_C4XE75", "uniref90_id": "UniRef90_C4XE75" }, "psc": { "uniref90_id": "UniRef90_C4XE75", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_A0A449B1R9", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_A0A449B1R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B1R9" ], "product": "Segregation and condensation protein A" }, "genes": [], "id": "IGIGPGHMMC_252", "locus": "GCA000148625_00214" }, { "type": "cds", "sequence": "contig_1", "start": 119054, "stop": 119638, "strand": "+", "frame": 2, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_013354377.1", "SO:0001217", "UniParc:UPI0001E32F85", "UniRef:UniRef100_UPI0001E32F85", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_C4XE76" ], "nt": "ATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACAAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAA", "aa": "MKNKILEALLYVQGDEGLTLIQVKEIFGLNTIAEARKVMNDFIKSYNDADGALKIVNFNEVYKLATRETYKEYITKMVQVVKKHRLSNAAIEVAGITAYKQPVTRSMINNIRGVQSDQVLNSLLTKGVIEEVGISPTPGNPILYGITNKFYDYFKIKSLAELQKLTEFNFVDGVENEAEDFNLFDSQRPENYDN", "aa_hexdigest": "4745bcf06f78214e3430924d40072b68", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32F85", "ncbi_nrp_id": "WP_013354377.1", "uniref100_id": "UniRef100_UPI0001E32F85", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F85", "RefSeq:WP_013354377.1", "UniRef:UniRef100_UPI0001E32F85" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F85", "uniref90_id": "UniRef90_C4XE76" }, "psc": { "uniref90_id": "UniRef90_C4XE76", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "IGIGPGHMMC_253", "locus": "GCA000148625_00215" }, { "type": "cds", "sequence": "contig_1", "start": 119692, "stop": 120531, "strand": "+", "frame": 1, "gene": null, "product": "RNA-binding S4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96", "UniRef:UniRef90_C4XE77" ], "nt": "ATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTATCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGCAACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACTAGCTTTTTTGAAAAAATATATAAACTAAAAAGTGATTTTAAAGAAGCTAAAAAACTTGTAGAAATTAATATTCAACACGATGAAAAATTATATAAAATGGTTGTTACAAATAATACTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATAAAATTTTTGCATCTATTTTAACAGGCAAAAAACATCAAATTAGATTAAGTATGGCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGGGGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGA", "aa": "MLEFIATKNDDGRKLISYLKRILINTPTSHIYKILRNKDIKINGVRINDPQYIINEKDIIQIYGIEANDYQNKKFENSAKKDFKIIYEDDNILIVSKPINLSVHSENNCLDKQVLSYLKYNQKDSFKPSHVGRLDKATSGLMIYAKNYATLVELNYKTSFFEKIYKLKSDFKEAKKLVEINIQHDEKLYKMVVTNNTKDPLAKTLFYTKNNKIFASILTGKKHQIRLSMAHLKTPIYGDIKYGGKKADRLYLHCYSITFKNLDNSLKYLNNKEFNDPIN", "aa_hexdigest": "99787d6d2e79440a6d6d127ca1373b21", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XE77", "query_cov": 1.0, "subject_cov": 0.9425675675675675, "identity": 0.9640000000000001, "score": 535.0, "evalue": 8.82e-192, "valid": true, "product": "RNA-binding S4 domain-containing protein", "uniref50_id": "UniRef50_A0A449AI96" }, "pscc": { "uniref50_id": "UniRef50_A0A449AI96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [], "id": "IGIGPGHMMC_254", "locus": "GCA000148625_00216" }, { "type": "cds", "sequence": "contig_1", "start": 120543, "stop": 120638, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGATGGAATTATGGCTGAATGA", "aa": "MKKVTKEELKKIVASLMLEPTNEVLDGIMAE", "aa_hexdigest": "fe47d054d042cecdc3277f9a14879ee8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3489.1708000000003, "isoelectric_point": 5.232790946960448 }, "id": "IGIGPGHMMC_255", "locus": "GCA000148625_00217" }, { "type": "cds", "sequence": "contig_1", "start": 120663, "stop": 120845, "strand": "+", "frame": 3, "gene": null, "product": "Glutamyl-tRNA amidotransferase subunit C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61", "UniRef:UniRef50_UPI0021AE6C6E", "UniRef:UniRef90_C4XE78" ], "nt": "ATGCTTAAAAAATTAGATACAACAAATGTTAAACCATTAAGCCACATTAATGAAGAAACTTTAGTTGACTTTTTAAGAGAAGATGTTGAAGACACTTCTTTTAGTATCAATAAACAAACTGCATTAAACAATGCTAAGGACAAAGATCAAGATTATATTATTACAACAAAGGTGGTGAAATAA", "aa": "MLKKLDTTNVKPLSHINEETLVDFLREDVEDTSFSINKQTALNNAKDKDQDYIITTKVVK", "aa_hexdigest": "a2c62d782b2ff7fb87c150ea1ba7c0ab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AE6C6E", "query_cov": 0.9333333333333333, "subject_cov": 1.0, "identity": 0.536, "score": 58.5, "evalue": 2.98e-10, "valid": false, "product": "Glu-tRNAGln amidotransferase C subunit", "uniref50_id": "UniRef50_UPI0021AE6C6E" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AE6C6E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AE6C6E" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 120528, "stop": 120845, "observations": { "mutation": [ 120636 ], "stop": [ 120636 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE78", "query_cov": 1.0, "subject_cov": 0.5714285714285714, "identity": 0.9329999999999999, "score": 108.0, "evalue": 2.4e-29, "gene_start": 1, "gene_end": 60, "reference_start": 46, "reference_end": 105, "reference_sequence": "MIRRIMKKVTKEELKKIVASLMLEPTNEVLDQIMTEWKVLNKNLNMLKKLNTTNVKPLSHINEEALVDFLREDVEDTSYSINKQTALNNAMDKDQDYIITTKVVK" }, "paralog": false, "identity": 0.9142857142857143, "score": 185.0, "evalue": 3.45e-62, "description": "Internal stop codon at 120636. Nonsense mutation around 120636.", "psc": { "uniref90_id": "UniRef90_C4XE78", "product": "Glutamyl-tRNA amidotransferase subunit C", "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE78", "UniRef:UniRef50_D4XV61" ] }, "pscc": { "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61" ], "product": "Glutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amidotransferase, C subunit" } }, "id": "IGIGPGHMMC_256", "locus": "GCA000148625_00218" }, { "type": "cds", "sequence": "contig_1", "start": 120845, "stop": 121747, "strand": "+", "frame": 2, "gene": null, "product": "amidase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "nt": "ATGAAACTACAAGTTTTAGGTGATTTTGATTTAGCTCAAGATGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGTTTTAAAAGAAGGCAATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCCACTAAAGATGCAAAAACAACAGCTTCAAGCTTGATTTTAGAAAATTTCAAACCTGGTTACAATGCAACTTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAAAAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGA", "aa": "MKLQVLGDFDLAQDELKKNKNNAVAYVYDKVLKEGNGLLNNAVFTIKEVFATKDAKTTASSLILENFKPGYNATCVQKLIDAGALKVAKVYCDELALGGTGTFSAFGLIKNPLDKERLAGGSSSGSVATFTDNISFALGSDTGDSVRLPASYNGNVGFKPSYGAISRYGMFAYASSLDTVSYFAHNVNDIAVISQVLFGVDNKDFTSKKVKIDNVQKLQPKKIGVLNVSKNLSNFVSNNFQELIVKLKANKNIKVEVIEPQEDLLNAVKAIYDIISYSEASSNLANLNGIAFGSRKEGES", "aa_hexdigest": "7b6b40dfba8b6e6421865d0ce1103798", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D5FF4", "query_cov": 0.83, "subject_cov": 0.9841897233201581, "identity": 0.51, "score": 230.0, "evalue": 1.47e-71, "valid": false, "product": "amidase family protein", "uniref50_id": "UniRef50_UPI00215D5FF4" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5FF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "product": "amidase family protein" }, "genes": [], "id": "IGIGPGHMMC_257", "locus": "GCA000148625_00219" }, { "type": "cds", "sequence": "contig_1", "start": 121757, "stop": 121897, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGA", "aa": "MTNTRSQGFGKMVQRRLTLGTFFLHSENQKDLFLKAQQGRRLFKEY", "aa_hexdigest": "c4269d1ec5cc4fa248b92c99039a2fde", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5508.303, "isoelectric_point": 11.000063133239745 }, "id": "IGIGPGHMMC_258", "locus": "GCA000148625_00220" }, { "type": "cds", "sequence": "contig_1", "start": 121904, "stop": 122050, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGA", "aa": "MHKQYDIVIYPAYADVAPYINKKNKNYKYMEYILTGSNLAGNPSITIP", "aa_hexdigest": "42b402ce2ced37fc65c407ffb3b23ffb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.311700000001, "isoelectric_point": 8.964216041564942 }, "id": "IGIGPGHMMC_259", "locus": "GCA000148625_00221" }, { "type": "cds", "sequence": "contig_1", "start": 122160, "stop": 122534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00202A5FF6", "PFAM:PF02934.20" ], "nt": "ATGAATAATTTTGAAGTAATAATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTTCACCTTGA", "aa": "MNNFEVIIGIEIHLELNTKTKMFSPARIDFNAEPNTTMNQIDLGYPGTLPLVNKEAVLSGIKLAKALNMKIDDELHFDRKNYFYPDLPKGYQITQFLRPIGSEGYVTINTDKGPKKIQIERISP", "aa_hexdigest": "7f58c138d33037010529fc58bcc12be6", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI00202A5FF6", "query_cov": 0.9919354838709677, "subject_cov": 0.8978102189781022, "identity": 0.585, "score": 145.0, "evalue": 2.03e-42, "valid": false, "uniref50_id": "UniRef50_UPI00202A5FF6" }, "pscc": { "uniref50_id": "UniRef50_UPI00202A5FF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00202A5FF6" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 117, "aa_cov": 0.9435483870967742, "hmm_cov": 1.0, "evalue": 9.351879987737662e-48, "score": 158.75425720214844, "start": 6, "stop": 122 } ], "seq_stats": { "molecular_weight": 14111.13249999998, "isoelectric_point": 6.730782508850097 }, "id": "IGIGPGHMMC_260", "locus": "GCA000148625_00222" }, { "type": "cds", "sequence": "contig_1", "start": 122473, "stop": 123048, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATGATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGA", "aa": "MSQLTLIKDLKKSKLKEFHLEEDTARQHHDEEGTKLDYNRAGVPLIEIVTHPVLRSADEAVAYVDMIRKIALSLNISDAKMEQGSLRADVNVSLRPYGYKGFGTKVEIKNMNSFRAIKNALEFEIDFQRKQILTNKPILQQTKRYDEATLSTVVMRTKTGEVDYKYFPEPNIPFIKLSKKLIDSVKLNELP", "aa_hexdigest": "799516d01d9ebca8befb543c78f8c635", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 175, "aa_cov": 0.9057591623036649, "hmm_cov": 0.96, "evalue": 3.503473006840241e-61, "score": 202.79583740234375, "start": 10, "stop": 182 } ], "seq_stats": { "molecular_weight": 21955.10460000001, "isoelectric_point": 9.079163169860841 }, "id": "IGIGPGHMMC_261", "locus": "GCA000148625_00223" }, { "type": "cds", "sequence": "contig_1", "start": 123274, "stop": 123411, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGA", "aa": "MDQDIISGRSFKKLVPLLVNFDGDFDKLVVDNNLKQISDEATITK", "aa_hexdigest": "61620e15f46e48a5677bbd62617ae68e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 44, "aa_cov": 0.9777777777777777, "hmm_cov": 1.0, "evalue": 1.3852485132327843e-07, "score": 27.535066604614258, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5081.7497, "isoelectric_point": 4.609039878845214 }, "id": "IGIGPGHMMC_262", "locus": "GCA000148625_00224" }, { "type": "cds", "sequence": "contig_1", "start": 123712, "stop": 124299, "strand": "+", "frame": 1, "gene": null, "product": "Abortive infection protein AbiGI", "db_xrefs": [ "RefSeq:WP_013354381.1", "SO:0001217", "UniParc:UPI0001A8C096", "UniRef:UniRef100_C4XE81", "UniRef:UniRef50_C4XE81", "UniRef:UniRef90_C4XE81" ], "nt": "ATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAA", "aa": "MQEEKVIKIGKKHNGVITTKECAMQRIHNQTLHRLLSKKIIEKISHGIYLLTEYLEDDHYLFQLRYPKTIFSYETALYLIHETDFLPINFDVSVTKGYKFNDKENIRVNYVSDELADLGKIKVKTMFNNDVYVYCYERVLCDLIKNRKKANLETYVNAIKRYAQYKNKNLDLLYEIAKKMNILDEMKNIMIGIIY", "aa_hexdigest": "0f07e925844bbe441d709750411fb93b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C096", "ncbi_nrp_id": "WP_013354381.1", "uniref100_id": "UniRef100_C4XE81", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C096", "RefSeq:WP_013354381.1", "UniRef:UniRef100_C4XE81" ] }, "ips": { "uniref100_id": "UniRef100_C4XE81", "uniref90_id": "UniRef90_C4XE81" }, "psc": { "uniref90_id": "UniRef90_C4XE81", "product": "Abortive infection protein AbiGI", "uniref50_id": "UniRef50_C4XE81" }, "pscc": { "uniref50_id": "UniRef50_C4XE81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE81" ], "product": "Abortive infection protein AbiGI" }, "genes": [], "id": "IGIGPGHMMC_263", "locus": "GCA000148625_00225" }, { "type": "cds", "sequence": "contig_1", "start": 124301, "stop": 124486, "strand": "+", "frame": 2, "gene": null, "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82", "UniRef:UniRef90_C4XE82" ], "nt": "ATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGA", "aa": "MTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYK", "aa_hexdigest": "ce0a3ea49624b32cb75c3919e102d53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 124295, "stop": 124618, "observations": { "mutation": [ 124484 ], "stop": [ 124484 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE82", "query_cov": 1.0, "subject_cov": 0.5700934579439252, "identity": 1.0, "score": 120.0, "evalue": 5.02e-34, "gene_start": 1, "gene_end": 61, "reference_start": 3, "reference_end": 63, "reference_sequence": "MNMTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYKWRTTVDLDFMSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKT" }, "paralog": false, "identity": 0.9813084112149533, "score": 209.0, "evalue": 7.57e-72, "description": "Internal stop codon at 124484. Nonsense mutation around 124484.", "psc": { "uniref90_id": "UniRef90_C4XE82", "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE82", "UniRef:UniRef50_C4XE82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82" ], "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein" } }, "id": "IGIGPGHMMC_264", "locus": "GCA000148625_00226" }, { "type": "cds", "sequence": "contig_1", "start": 124511, "stop": 124849, "strand": "+", "frame": 2, "gene": null, "product": "nucleotidyl transferase AbiEii/AbiGii toxin family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F89", "UniRef:UniRef90_UPI0001E32F89" ], "nt": "ATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACAGAACCAATAAATGTTAATAATGAAAAGAAAGGAATTAGATTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGA", "aa": "MSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKTEPINVNNEKKGIRLYVKVKIKNITEIVPLDIVEESKIDPTYISKEFNSIYGENFTFLIYNIENVIAEKVFAIYTNRF", "aa_hexdigest": "b265dee9949ba5f0c4a06fa0ea557e52", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 124301, "stop": 125140, "observations": { "mutation": [ 124484, 124847, 125036, 125063 ], "stop": [ 124484, 124847, 125036, 125063 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32F89", "query_cov": 1.0, "subject_cov": 0.4014336917562724, "identity": 1.0, "score": 216.0, "evalue": 8.03e-69, "gene_start": 1, "gene_end": 112, "reference_start": 71, "reference_end": 182, "reference_sequence": "MTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYKWRTTVDLDFMSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKTEPINVNNEKKGIRLYVKVKIKNITEIVPLDIVEESKIDPTYISKEFNSIYGENFTFLIYNIENVIAEKVFAIYTNRFWNSRLKDFFDIYYLFENQKKFINKEILKKAIDNAFKVRNASFDKTIFIDLLNALKISELQNLQWEKLVLSKNWQTSKSFGEIVEVIIKHFENDIENV" }, "paralog": false, "identity": 0.985663082437276, "score": 533.0, "evalue": 4.53e-196, "description": "Internal stop codon at 124484, 124847, 125036, 125063. Nonsense mutation around 124484, 124847, 125036, 125063.", "psc": { "uniref90_id": "UniRef90_UPI0001E32F89", "product": "nucleotidyl transferase AbiEii/AbiGii toxin family protein", "uniref50_id": "UniRef50_UPI0001E32F89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32F89", "UniRef:UniRef50_UPI0001E32F89" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F89" ], "product": "nucleotidyl transferase AbiEii/AbiGii toxin family protein" } }, "id": "IGIGPGHMMC_265", "locus": "GCA000148625_00227" }, { "type": "cds", "sequence": "contig_1", "start": 125378, "stop": 125671, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTAGTTGAAAAATATATTCAACAAGCTGGATATGTTGAAAACAAAACATATTTTAAACAAATATATTCAAGTGAGATCTATGAAAAGTTTGGAATTAATTTAAATAATATTCAAGAAACAACTGCAAATAAAAGATTTGATTTTGGAATCATTAAAAATCATAAATTTTATGCAATTGAAGTAAATTTTTATACGGCTCAAGGTTCTAAACTTAATGAAGTTTCAAGAAGCTATGAAAAAATTTATTTATCTACAAAAACAATTACTAATTTTGAATTCATTTGA", "aa": "MEKLVEKYIQQAGYVENKTYFKQIYSSEIYEKFGINLNNIQETTANKRFDFGIIKNHKFYAIEVNFYTAQGSKLNEVSRSYEKIYLSTKTITNFEFI", "aa_hexdigest": "f4fe23e10362a9884fb675aa0d21918a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11564.959199999988, "isoelectric_point": 8.730066871643068 }, "id": "IGIGPGHMMC_266", "locus": "GCA000148625_00228" }, { "type": "cds", "sequence": "contig_1", "start": 126101, "stop": 127150, "strand": "-", "frame": 1, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "RefSeq:WP_013354384.1", "SO:0001217", "UniParc:UPI0001E32F8B", "UniRef:UniRef100_UPI0001E32F8B", "UniRef:UniRef50_A0A1V5HP79", "UniRef:UniRef90_C4XE85" ], "nt": "ATGGAAACACTTTTTAAAACAATTGGCGACAATATTAAAATGGAAAGACTTGCAAAAAATATGACACAACAAGAATTAGCAGAATTAAGTAGCACCGAAAGATCTCAATTAACTAAAATTGAAAAAGGTCAAATTAAGGGCGTTCAGTTTTTTACAATTTGCAAAATATATAATGCTTTGAATTTGAATAATATTTATATTGGAAAATTAAACACAAAACTTTCACCATTTGTAAAATGGGCTGGTGACAAAACTCAATTATTAGATAAACTTAAAAGTTTAATGCCAAAAACATATAACAATTATTTGGAACCTTTTGTTGGTGGCGGAGCATTATTACTAAATCTAAAGCCGCAAAATTTCATAATAAATGATTTTAATAAAGAATTAATTAATGTTTTTAATTGCTTTAAAAATGATAAAGATTTTAAATTATTAAAAAAAGAATTAATTAATCATGAAAACAATCATAATGATGGGTATTACTATAAAATAAGAGATTTGGACAAATTAGATAAATACGATAAACTGCCAAATTACAAAAAAGCTGCCCGAACAATATACTTAAATAAAGCATGTTTTAATGGTTTATACCGAGTAAATTCTAAAGGACAATTTAATGTACCTTCTGGCAAGAAAAACTCAGTTAATTGCTTTGATAGAGACAATTTTGAAAATTTAAAAAACTTTTTAAAAACTACAAATAATCAAATTTTCTGTGAAGATTTTGAAGTATTTTGTGAAAAAGCTCAAAAAGGAGATTTTGTTTATTTTGATCCTCCTTATGATGTCATTGAAAACAAAAATACTTTCACTTCATATAATTCAGAAAGTTTTGGAAAAGAAGAACAAAAACGATTATCCGAAGTTTTTAAAAAACTTGACAAAAAAGGTGTCAAAGTAATGTTAAGTAATCACAACACAAAATATATTAATGAATTATATGCAGACTTTAATATTAATGTTGTTAAAGCAAAAAGAATGATAAATTCAAATGGAAGTGGACGAGGAGAAGTTGAAGAAGTAATAATTACAAATTATAGGGAGTAA", "aa": "METLFKTIGDNIKMERLAKNMTQQELAELSSTERSQLTKIEKGQIKGVQFFTICKIYNALNLNNIYIGKLNTKLSPFVKWAGDKTQLLDKLKSLMPKTYNNYLEPFVGGGALLLNLKPQNFIINDFNKELINVFNCFKNDKDFKLLKKELINHENNHNDGYYYKIRDLDKLDKYDKLPNYKKAARTIYLNKACFNGLYRVNSKGQFNVPSGKKNSVNCFDRDNFENLKNFLKTTNNQIFCEDFEVFCEKAQKGDFVYFDPPYDVIENKNTFTSYNSESFGKEEQKRLSEVFKKLDKKGVKVMLSNHNTKYINELYADFNINVVKAKRMINSNGSGRGEVEEVIITNYRE", "aa_hexdigest": "5460fc339a8161cd11c29baeeb2f4c79", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F8B", "ncbi_nrp_id": "WP_013354384.1", "uniref100_id": "UniRef100_UPI0001E32F8B", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F8B", "RefSeq:WP_013354384.1", "UniRef:UniRef100_UPI0001E32F8B" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F8B", "uniref90_id": "UniRef90_C4XE85" }, "psc": { "uniref90_id": "UniRef90_C4XE85", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A1V5HP79", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HP79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HP79" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "IGIGPGHMMC_267", "locus": "GCA000148625_00229" }, { "type": "cds", "sequence": "contig_1", "start": 127237, "stop": 127407, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13412.12", "PFAM:PF13730.12", "PFAM:PF01978.25" ], "nt": "TTGCCAGATTCTGCTTATTATGTATATATGGCTTTAAAAAATAATCCAAAAGAAACAGCTGGTTCACTAGCTAAAAAAATAAAAAAATCTTCGAGAACTATTCAAAGAATTTTAGATCTTTTAAAAGACAAGGGCTACATAGCTAGAATTGGTAAGACAAGAGGATATTGA", "aa": "MPDSAYYVYMALKNNPKETAGSLAKKIKKSSRTIQRILDLLKDKGYIARIGKTRGY", "aa_hexdigest": "7ef3bf57f3b285762e7fce32fe31178b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13412.12", "name": "HTH_24", "length": 37, "aa_cov": 0.6607142857142857, "hmm_cov": 1.0, "evalue": 1.4952611150948825e-06, "score": 23.758155822753906, "start": 11, "stop": 47 }, { "id": "PF13730.12", "name": "HTH_36", "length": 35, "aa_cov": 0.5714285714285714, "hmm_cov": 1.0, "evalue": 2.611823807109874e-07, "score": 26.415742874145508, "start": 16, "stop": 47 }, { "id": "PF01978.25", "name": "TrmB", "length": 45, "aa_cov": 0.8035714285714286, "hmm_cov": 1.0, "evalue": 4.169631639142471e-07, "score": 25.81563377380371, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6365.430400000001, "isoelectric_point": 10.118457984924316 }, "id": "IGIGPGHMMC_268", "locus": "GCA000148625_00230" }, { "type": "cds", "sequence": "contig_1", "start": 127408, "stop": 127587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTAGTTGATGAAGCTAAAATTTGTAATAATGGTTTCAAGAGAGTATATGAAGAATGTGAAAAAACTAATGTTAAAATTACTTATCAAAAAGGCAATTATGGTTTTGCATTTATATTTTTAAGAGAAAACAAAAGTTTAATTAGTAAAGATGAAGAATTTAAAGAATACTGA", "aa": "MINLVDEAKICNNGFKRVYEECEKTNVKITYQKGNYGFAFIFLRENKSLISKDEEFKEY", "aa_hexdigest": "483147132869ca9d8d56b1229ce78947", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7049.966199999999, "isoelectric_point": 6.222414588928221 }, "id": "IGIGPGHMMC_269", "locus": "GCA000148625_00231" }, { "type": "cds", "sequence": "contig_1", "start": 127831, "stop": 128016, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGAAAAACTAACAAATGCTGCTCATTATCTTTTTTCGCATAGAGAACCTATAGTTTTAAAAATGGCTGTTTTTGCAACAGATCAAAAATTAACATTTTTAGATATCAATAGAATTTCTGGTAACATCTTTCAATTAATTGAGAAAGCTAATGAATATGTTAAATCTAAAATCAATTGA", "aa": "MKNEKLTNAAHYLFSHREPIVLKMAVFATDQKLTFLDINRISGNIFQLIEKANEYVKSKIN", "aa_hexdigest": "f8413889cba5f0f0837b8715b90f8b0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7096.193599999999, "isoelectric_point": 9.455529594421389 }, "id": "IGIGPGHMMC_270", "locus": "GCA000148625_00232" }, { "type": "cds", "sequence": "contig_1", "start": 128155, "stop": 128553, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04326.19" ], "nt": "ATGAATTTTGGAATCGAAAGCGAAACACTTGAATTTAAGAAAAATACAAATGAAATAAATGAGGCTATGAAATCAATTTGTGCCATGTTAAATAAACACGGAAAAGGCACATTATATTTTGGCATCGGAGATGATGGATTAGTATATGATCAAAAAATCAACATAAATACTTTAAGAGATATTTGCAGAAAAATTTATGAAAATATTGAACCTGTAATTAGTCCTATTATTGAGAAAAAAATTGTTGATAATAAACAAATAATAGAGGTAACTTTTTGTGGGAATGATGCTCCATATTCAGTTGAAGGCATCTATTATATTAGAAATGATGATGAGGATAAAATACTCAGTCCTAATCAATTAAGACAGCTTTTTGAATTCAATAAAAACAAAAGCTGA", "aa": "MNFGIESETLEFKKNTNEINEAMKSICAMLNKHGKGTLYFGIGDDGLVYDQKININTLRDICRKIYENIEPVISPIIEKKIVDNKQIIEVTFCGNDAPYSVEGIYYIRNDDEDKILSPNQLRQLFEFNKNKS", "aa_hexdigest": "c41e6403b0996c4f92f8cefef0c78054", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04326.19", "name": "SLFN_AlbA_2", "length": 117, "aa_cov": 0.8333333333333334, "hmm_cov": 0.9914529914529915, "evalue": 3.6285220003771065e-21, "score": 71.92584991455078, "start": 6, "stop": 115 } ], "seq_stats": { "molecular_weight": 15230.18459999999, "isoelectric_point": 5.072220802307128 }, "id": "IGIGPGHMMC_271", "locus": "GCA000148625_00233" }, { "type": "cds", "sequence": "contig_1", "start": 128702, "stop": 128989, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTGATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGA", "aa": "MKQYKNIKEVEEKYTFDLEDILKGQTIDDLFNEFIELSKIALKTKDTKYENVENYIKSVELGKDMGVLSNRIHNYLSNHSNQNLVDSKWTDLSTK", "aa_hexdigest": "6086e5c4cb7b3c7aebf9800ec85ae62a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11192.475499999988, "isoelectric_point": 5.350447654724121 }, "id": "IGIGPGHMMC_272", "locus": "GCA000148625_00234" }, { "type": "cds", "sequence": "contig_1", "start": 129167, "stop": 129745, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACATGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGA", "aa": "MNQSSSANPDYESIFNIISDSELDYGFATDSKGKKYKLSPAIKAKFMKKDDFKIRKSTHDSFLKAHMKHKDSMANLLYQHFKGLTVTAKIRKYKNTIDMLTHSDKVDDSMLQFLFDKVSTLKHTIKNRNKYYKKFYEAKFKEKYHAKYDSYRELVKVKSTYTVEQMQNIVSEALKPFGSEYHKMITKAINER", "aa_hexdigest": "4eef21baae5d3a2e4ec55da617d30c49", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22633.778300000013, "isoelectric_point": 9.640167045593262 }, "id": "IGIGPGHMMC_273", "locus": "GCA000148625_00235" }, { "type": "cds", "sequence": "contig_1", "start": 129755, "stop": 130093, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGATGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGA", "aa": "MTVDNKLSGAYSIGNTYGLDKKYILMNFDGELGSVETLAHELGHSMHSYFSDKNNDISNASYPIILAEIASIFNELMLYNYLLKTSKNDLFKFKILDNMIDGFVGTVFRQIL", "aa_hexdigest": "4883482d946ef3c0ce0354fe6dd4bc16", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12672.344699999985, "isoelectric_point": 5.1619695663452125 }, "id": "IGIGPGHMMC_274", "locus": "GCA000148625_00236" }, { "type": "cds", "sequence": "contig_1", "start": 130187, "stop": 130492, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGA", "aa": "MKYATKKIKYKKEKNIMSVYVPHYYYGFYVYKYAIGQLVANYFYTRVKNEGEKYLQVYINDFLSAGDRDYPLETLKKVGADLKDPQFYEIGFSYFKEIVKE", "aa_hexdigest": "4d2b96b4d73b5534a500c5b01b53f353", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12170.87519999999, "isoelectric_point": 9.17128849029541 }, "id": "IGIGPGHMMC_275", "locus": "GCA000148625_00237" }, { "type": "cds", "sequence": "contig_1", "start": 130835, "stop": 130975, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGA", "aa": "MNLIFAFRNQKRYKRDFADFILKKYKKATKAAFLPFLISTLAFYLA", "aa_hexdigest": "e553bb910996a9e17b526de4f0921ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.583899999999, "isoelectric_point": 10.354412269592284 }, "id": "IGIGPGHMMC_276", "locus": "GCA000148625_00238" }, { "type": "cds", "sequence": "contig_1", "start": 131270, "stop": 131380, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATCAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGA", "aa": "MISIIAAILNIVLTLSFQHLHNQIFGDLKNLLPIKE", "aa_hexdigest": "4ba0ee04945a45c1b7f24c9baa578c93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4071.8679, "isoelectric_point": 6.703556632995605 }, "id": "IGIGPGHMMC_277", "locus": "GCA000148625_00239" }, { "type": "cds", "sequence": "contig_1", "start": 131906, "stop": 132103, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGA", "aa": "MKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIED", "aa_hexdigest": "378f1d9c066f4018d6a3a668c29f5142", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7653.0238999999965, "isoelectric_point": 7.911253929138184 }, "id": "IGIGPGHMMC_278", "locus": "GCA000148625_00240" }, { "type": "cds", "sequence": "contig_1", "start": 132470, "stop": 133183, "strand": "+", "frame": 2, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_C4XE89", "UniRef:UniRef90_C4XE89" ], "nt": "ATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTGTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAA", "aa": "MSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEALSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN", "aa_hexdigest": "fd79a4319fcf278f90f1744197601759", "start_type": "ATG", "rbs_motif": null, "genes": [ "tig" ], "truncated": "5-prime", "pseudogene": { "start": 132170, "stop": 133183, "observations": { "mutation": [ 132314 ], "stop": [ 132314 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE89", "query_cov": 1.0, "subject_cov": 0.4637964774951076, "identity": 0.996, "score": 421.0, "evalue": 4.34e-144, "gene_start": 1, "gene_end": 237, "reference_start": 275, "reference_end": 511, "reference_sequence": "MRRIMAENNKKFTIDEKKSELVYTLEINGKEWEKLYADAKAKEVKNIKIPGFRPGKAPQHEIEKRVNPLSVASEVVDQYWRNNHDRLKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIEDWIAQQLEKNALLLPLKKDEKTKDGDTVTIDYKGFIKDEPFDGGEAQGFDLKLGSHTFIDTFEDQLIGKKIGWKGEVKVTFPKEYAVEKLKGQPATFEVEIKDAKRREKVELTDKNFGTVRLAKMSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEAFSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN" }, "paralog": false, "identity": 0.9940652818991098, "score": 651.0, "evalue": 3.85e-237, "description": "Internal stop codon at 132314. Nonsense mutation around 132314.", "psc": { "uniref90_id": "UniRef90_C4XE89", "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_C4XE89", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE89", "EC:5.2.1.8", "UniRef:UniRef50_C4XE89", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE89" ], "product": "Trigger factor" } }, "id": "IGIGPGHMMC_279", "locus": "GCA000148625_00241" }, { "type": "cds", "sequence": "contig_1", "start": 133310, "stop": 133618, "strand": "+", "frame": 2, "gene": null, "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "nt": "ATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCATATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGA", "aa": "MLNIVLFEPEISPNTGNIIRTCFALGAKLHIIKPIGFDLDPKYLSRPAAGRLLSDIQHEVHASYKDFYEKYKNKNIFYLTRYGLKTYTDVDFKKEYKKNKEV", "aa_hexdigest": "22b6a188d1e5174ba9a3513e0f65bdfc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A449AH69", "query_cov": 0.9607843137254902, "subject_cov": 0.9514563106796117, "identity": 0.7240000000000001, "score": 150.0, "evalue": 1.55e-45, "valid": false, "product": "tRNA/rRNA methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A449AH69", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [], "id": "IGIGPGHMMC_280", "locus": "GCA000148625_00242" }, { "type": "cds", "sequence": "contig_1", "start": 133622, "stop": 133843, "strand": "+", "frame": 2, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGCTAAGTCTATATGAAGTTCAAAAAGGAAAGGATTTCTTAATCAAATGGAAAAAACAGTAA", "aa": "MFGKESTGIDKEILQTHLENCLRIPMMSEMRSINLANTVAIVGFEIMRQLDFKELSLYEVQKGKDFLIKWKKQ", "aa_hexdigest": "57d40f84f2e8ab4431ba3a570ef850f3", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 0.958904109589041, "subject_cov": 0.8433734939759037, "identity": 0.643, "score": 94.7, "evalue": 4.76e-24, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "IGIGPGHMMC_281", "locus": "GCA000148625_00243" }, { "type": "cds", "sequence": "contig_1", "start": 133828, "stop": 134541, "strand": "+", "frame": 1, "gene": "spoU", "product": "SpoU rRNA Methylase family", "db_xrefs": [ "RefSeq:WP_013354390.1", "SO:0001217", "UniParc:UPI0001E32F90", "UniRef:UniRef100_C4XE91", "UniRef:UniRef50_A0A3P8KX10", "UniRef:UniRef90_C4XE91" ], "nt": "ATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAA", "aa": "MEKTVISSVSNPKIKYLKEVQKDNKSDYFLVSGYHLVQEALAKNLVIEIFEEESKSTYKTATLVKSNVLKSISSTMHPDGVVALCLKPKSQIKNKVILLDNVQDPGNVGTIIRNAACFGFDTVISNVNFYNDKVVRATQGALFQVSLIKENNNEDLIKVLNNEGYFIVATSLDKDSIKLDELKINKEKVVLILGNEGNGINKNLYKYCDQKVYIPIEFESLNVAVASGIILNKIYNK", "aa_hexdigest": "49962aab1b0023aa589850a65d2a39c5", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F90", "ncbi_nrp_id": "WP_013354390.1", "uniref100_id": "UniRef100_C4XE91", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F90", "RefSeq:WP_013354390.1", "UniRef:UniRef100_C4XE91" ] }, "ips": { "uniref100_id": "UniRef100_C4XE91", "uniref90_id": "UniRef90_C4XE91" }, "psc": { "uniref90_id": "UniRef90_C4XE91", "product": "SpoU rRNA Methylase family", "uniref50_id": "UniRef50_A0A3P8KX10" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KX10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KX10" ], "product": "tRNA/rRNA methylase" }, "genes": [ "spoU" ], "id": "IGIGPGHMMC_282", "locus": "GCA000148625_00244" }, { "type": "cds", "sequence": "contig_1", "start": 134553, "stop": 134654, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGA", "aa": "MKKTNNEKFFNKNFSRELNGYSVPEVDNFIDQL", "aa_hexdigest": "8611b7902e270cc7485b45ba406ab0c1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3967.376000000001, "isoelectric_point": 6.038369941711425 }, "id": "IGIGPGHMMC_283", "locus": "GCA000148625_00245" }, { "type": "cds", "sequence": "contig_1", "start": 134851, "stop": 135069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGA", "aa": "MAKAMREIKENATLADIFLIVLDARIPISSYNEDFDNIAPQKPRLFVITKSDLMDKSKKEKIQKRFQNEKLL", "aa_hexdigest": "8eca640255274886bdac0eb287ae0541", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8410.785899999999, "isoelectric_point": 9.345675468444824 }, "id": "IGIGPGHMMC_284", "locus": "GCA000148625_00246" }, { "type": "cds", "sequence": "contig_1", "start": 135322, "stop": 135627, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGA", "aa": "MDTPGILLPKHEDQSAAIKLAMIGSIETNIFPQRFLAENFLKVIVKYYPNKIKEEFNIDIESKDDLSETEAYNIVAQIADNKNFKVDKKIDFDRTYKYILN", "aa_hexdigest": "4bd537d463d1bbe23bd18d8bac485910", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11745.310899999982, "isoelectric_point": 5.1215002059936525 }, "id": "IGIGPGHMMC_285", "locus": "GCA000148625_00247" }, { "type": "cds", "sequence": "contig_1", "start": 135670, "stop": 135858, "strand": "+", "frame": 1, "gene": null, "product": "Phage protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94", "UniRef:UniRef90_C4XE94" ], "nt": "ATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTAAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGA", "aa": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYA", "aa_hexdigest": "d1022672db1a9e878e1e3842c3b10eff", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 135670, "stop": 136101, "observations": { "mutation": [ 135856, 135901 ], "stop": [ 135856, 135901 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE94", "query_cov": 1.0, "subject_cov": 0.43356643356643354, "identity": 1.0, "score": 111.0, "evalue": 4.72e-30, "gene_start": 1, "gene_end": 62, "reference_start": 1, "reference_end": 62, "reference_sequence": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYAWQNKAAPSITYTTLLWILICVFSILTILSLYLLILFFIEYSLIKKIGLKKSEQEIEASIRKFVKFGFKKYPKKQMEMLEKF" }, "paralog": false, "identity": 0.972027972027972, "score": 267.0, "evalue": 4.22e-94, "description": "Internal stop codon at 135856, 135901. Nonsense mutation around 135856, 135901.", "psc": { "uniref90_id": "UniRef90_C4XE94", "product": "Phage protein", "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE94", "UniRef:UniRef50_C4XE94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94" ], "product": "Phage protein" } }, "id": "IGIGPGHMMC_286", "locus": "GCA000148625_00248" }, { "type": "cds", "sequence": "contig_1", "start": 136227, "stop": 137078, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter related protein", "db_xrefs": [ "RefSeq:WP_013354394.1", "SO:0001217", "UniParc:UPI0001E32F93", "UniRef:UniRef100_UPI0001E32F93", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_A0AB32XAQ9" ], "nt": "ATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTCAAAACATTGCATTATTAGGATCAAATGGAGCTGGTAAAACCACAACAGTTGAAATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAATTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGCCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATCAACTTTATTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTTGCAGCTTGCTTAGAGTACTACATTTAA", "aa": "MPKLTETQETRMSNSIKEFVENLEKHENRILNHPAIEPREYDGLEKLIEIKGLCKEYKIKGKKFRAVDDMNLTLYKSQNIALLGSNGAGKTTTVEMLVGILKPSAGNIKYFFGDSMDKGKIDATKIGIQFQDSSYPQGLIVRDVIKSMNKIYGNKMKGLELDYLIKIFGVDEYIDNKAASLSGGQHQRLNALLALIHKPELVILDELSTGLDIKIKTRLVEFINNFVKDNKSTLLIVSHDINEINTLADRIVIMNKGKVIFDKTKDEAVKEFKTLAACLEYYI", "aa_hexdigest": "376598ab21d2fa9946693068419096a8", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001E32F93", "ncbi_nrp_id": "WP_013354394.1", "uniref100_id": "UniRef100_UPI0001E32F93", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F93", "RefSeq:WP_013354394.1", "UniRef:UniRef100_UPI0001E32F93" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F93", "uniref90_id": "UniRef90_A0AB32XAQ9" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAQ9", "product": "ABC transporter related protein", "uniref50_id": "UniRef50_D1J8M4" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_287", "locus": "GCA000148625_00249" }, { "type": "cds", "sequence": "contig_1", "start": 137087, "stop": 137221, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGA", "aa": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHF", "aa_hexdigest": "524f818323dd96e7ee2b338dfc14b92b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5298.046899999999, "isoelectric_point": 10.118586921691893 }, "id": "IGIGPGHMMC_288", "locus": "GCA000148625_00250" }, { "type": "cds", "sequence": "contig_1", "start": 137303, "stop": 137491, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTTGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTGGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGGTATTTGA", "aa": "MILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSGI", "aa_hexdigest": "3a4e36049cfcafd05f64f7b6acaf5562", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6708.0404, "isoelectric_point": 9.655059242248537 }, "id": "IGIGPGHMMC_289", "locus": "GCA000148625_00251" }, { "type": "cds", "sequence": "contig_1", "start": 137510, "stop": 137629, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGA", "aa": "MVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIH", "aa_hexdigest": "89a559746d51c1689a36966f10a29b8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4509.1675, "isoelectric_point": 8.274984550476077 }, "id": "IGIGPGHMMC_290", "locus": "GCA000148625_00252" }, { "type": "cds", "sequence": "contig_1", "start": 137864, "stop": 138673, "strand": "+", "frame": 2, "gene": null, "product": "ABC-2 type transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97", "UniRef:UniRef50_UPI000FF82EAB", "UniRef:UniRef90_C4XE97", "UniRef:UniRef90_UPI000FF82EAB" ], "nt": "ATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGGAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGA", "aa": "MAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENGNDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILP", "aa_hexdigest": "873cf0d2aa0f33e4a05c8df6b2858c1c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82EAB", "query_cov": 0.9330855018587361, "subject_cov": 0.9061371841155235, "identity": 0.996, "score": 479.0, "evalue": 7.43e-170, "valid": true, "uniref50_id": "UniRef50_UPI000FF82EAB" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82EAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82EAB" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 137564, "stop": 138733, "observations": { "mutation": [ 137627, 137813, 138671, 138677, 138719 ], "stop": [ 137627, 137813, 138671, 138677, 138719 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE97", "query_cov": 1.0, "subject_cov": 0.4908759124087591, "identity": 0.996, "score": 513.0, "evalue": 2.07e-179, "gene_start": 1, "gene_end": 269, "reference_start": 260, "reference_end": 528, "reference_sequence": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHFWKAFVGPFFAFGYPIVFVIILGAVFNYEMILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSAIWTALVALMVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIHWAGYIYSAIVLTLVSIAVGLFIVSVSKSILTIQAIGSSLLIISMFLTGQVLPISQIIGQKVMWYLSYITPFKSPITQNTMAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENENDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILPWLWIVVLVGLSQKSFTWNTR" }, "paralog": false, "identity": 0.9845758354755784, "score": 733.0, "evalue": 2.07e-268, "description": "Internal stop codon at 137627, 137813, 138671, 138677, 138719. Nonsense mutation around 137627, 137813, 138671, 138677, 138719.", "psc": { "uniref90_id": "UniRef90_C4XE97", "product": "ABC-2 type transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE97", "UniRef:UniRef50_C4XE97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97" ], "product": "ABC-2 type transporter domain-containing protein" } }, "id": "IGIGPGHMMC_291", "locus": "GCA000148625_00253" }, { "type": "cds", "sequence": "contig_1", "start": 138835, "stop": 139005, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98", "UniRef:UniRef90_C4XE98" ], "nt": "TTGTTTAAAAAATATAAACTTGAAATTCAAATAGATTTAACTAATGATAAAGAAGATCTTGAAAAAAGAAACCATACAACAGCTAATAAATATATGGATTTAGTCTATAAAGTCAAAATTGCTGAAAAAAAGATTAAAGAAGGTTTTGCTGCATATTTAAAAATGCTATGA", "aa": "MFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKML", "aa_hexdigest": "c98de4fdb68c65f42dc564070b0ffdba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 138826, "stop": 139092, "observations": { "mutation": [ 138837, 139038 ], "stop": [ 138837, 139038 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE98", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.982, "score": 108.0, "evalue": 8.74e-30, "gene_start": 1, "gene_end": 56, "reference_start": 30, "reference_end": 85, "reference_sequence": "MYNNKKVFPFIKQEFNENWKLLTKEIVQNLFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKMLWNV" }, "paralog": false, "identity": 0.9659090909090909, "score": 169.0, "evalue": 2.03e-56, "description": "Internal stop codon at 138837, 139038. Nonsense mutation around 138837, 139038.", "psc": { "uniref90_id": "UniRef90_C4XE98", "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE98", "UniRef:UniRef50_C4XE98" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6740.905500000001, "isoelectric_point": 9.363597679138184 }, "id": "IGIGPGHMMC_292", "locus": "GCA000148625_00254" }, { "type": "cds", "sequence": "contig_1", "start": 139105, "stop": 139221, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43", "UniRef:UniRef50_A0AB32XB43", "UniRef:UniRef90_A0AB32XB43" ], "nt": "ATGAATAGTGCATTAAGTACTTTAGCAACCTTACTTTTAACCAGTGCTTCATGCATTAAAGAAGACAGAATGTATGACAAGCCTGCTAAAGAAATTGATAAATATAAGCAGCTTTAA", "aa": "MNSALSTLATLLLTSASCIKEDRMYDKPAKEIDKYKQL", "aa_hexdigest": "ed80be0d1b049de55233c5bf81ab5965", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59E84", "uniref100_id": "UniRef100_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB43", "uniref90_id": "UniRef90_A0AB32XB43" }, "psc": { "uniref90_id": "UniRef90_A0AB32XB43", "uniref50_id": "UniRef50_A0AB32XB43" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XB43" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4290.974300000002, "isoelectric_point": 7.869091606140137 }, "id": "IGIGPGHMMC_293", "locus": "GCA000148625_00255" }, { "type": "cds", "sequence": "contig_1", "start": 139342, "stop": 140049, "strand": "+", "frame": 1, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013354397.1", "SO:0001217", "UniParc:UPI0001E32F95", "UniRef:UniRef100_UPI0001E32F95", "UniRef:UniRef50_C5J6Z6", "UniRef:UniRef90_C4XE99" ], "nt": "ATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAAGCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAA", "aa": "MPTPHISAQKGEIAKIVLMPGDPLRAEYMAKKFLKNPKLVSSVRNVLFYTGKYNGKDVTIGASGMGSASIGIYAYELFTVYEVETIVRVGSTGSYIKELNVKQPVLVKRAYADGTGFVELMVGNKKQENYPDLETYEKLKQTSKEMNVELKEVSCHSTDVFYGLRSLEDTIKITKCQTVDNECFALFATAQRCKKKAAALLSVSENIVTGASLSSDERLKEFSTMFLVALNALTK", "aa_hexdigest": "2090d26d5ba17a389319d1f13e48f92f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32F95", "ncbi_nrp_id": "WP_013354397.1", "uniref100_id": "UniRef100_UPI0001E32F95", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F95", "RefSeq:WP_013354397.1", "UniRef:UniRef100_UPI0001E32F95" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F95", "uniref90_id": "UniRef90_C4XE99" }, "psc": { "uniref90_id": "UniRef90_C4XE99", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_C5J6Z6", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_C5J6Z6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6Z6" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "IGIGPGHMMC_294", "locus": "GCA000148625_00256" }, { "type": "cds", "sequence": "contig_1", "start": 140060, "stop": 140569, "strand": "+", "frame": 2, "gene": "tpx", "product": "thiol peroxidase", "db_xrefs": [ "EC:1.11.1.-", "EC:1.11.1.24", "GO:0008379", "GO:0016684", "KEGG:K11065", "RefSeq:WP_013354398.1", "SO:0001217", "UniParc:UPI0001A8C0CB", "UniRef:UniRef100_C4XEA0", "UniRef:UniRef50_A8MM68", "UniRef:UniRef90_C4XEA0" ], "nt": "ATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGGCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGCGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAG", "aa": "MNKRQETTFFKNKVTLLGDKIEVGQKAPNFTALNNELKSVQLKDFKDKVILISSVPSLDTGVCQMQTVRFNKEVEQIKGLKVITISCDLPFAQSRFCTSFNTNNVVTLSDHKNLDFGLKYGCVIEELRLLNRAIFVIDKNGIIQYVEYVKENTDHPNYDKALKCVKNII", "aa_hexdigest": "2cd7c50fc38f1dd5528e8b82e42a6e94", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C0CB", "ncbi_nrp_id": "WP_013354398.1", "uniref100_id": "UniRef100_C4XEA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0CB", "RefSeq:WP_013354398.1", "UniRef:UniRef100_C4XEA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEA0", "uniref90_id": "UniRef90_C4XEA0" }, "psc": { "uniref90_id": "UniRef90_C4XEA0", "gene": "tpx", "product": "thiol peroxidase", "ec_ids": [ "1.11.1.-", "1.11.1.24" ], "uniref50_id": "UniRef50_A8MM68", "kegg_orthology_id": "K11065", "go_ids": [ "GO:0008379", "GO:0016684" ] }, "pscc": { "uniref50_id": "UniRef50_A8MM68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A8MM68" ], "product": "Thiol peroxidase" }, "genes": [ "tpx" ], "id": "IGIGPGHMMC_295", "locus": "GCA000148625_00257" }, { "type": "cds", "sequence": "contig_1", "start": 140902, "stop": 141147, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1", "UniRef:UniRef90_C4XEA1" ], "nt": "ATGGAGGAAAATTATGAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGA", "aa": "MEENYEKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKD", "aa_hexdigest": "efc697ec3bdc51e271486e769886cd1e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 140917, "stop": 141265, "observations": { "insertion": [ 141193 ], "mutation": [ 141145, 141172 ], "stop": [ 141145, 141172, 141233 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA1", "query_cov": 0.9382716049382716, "subject_cov": 0.6495726495726496, "identity": 0.987, "score": 144.0, "evalue": 3.93e-43, "gene_start": 6, "gene_end": 81, "reference_start": 3, "reference_end": 78, "reference_sequence": "MMKKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKDWAKEFLSKDWDKNKNTKKVIIFNLKRFLKWTTEELNKYQ" }, "paralog": false, "identity": 0.9565217391304348, "score": 209.0, "evalue": 2.07e-71, "description": "Internal stop codon at 141145, 141172, 141233. Frameshift due to insertion around 141193. Nonsense mutation around 141145, 141172.", "psc": { "uniref90_id": "UniRef90_C4XEA1", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA1", "UniRef:UniRef50_C4XEA1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1" ], "product": "Lipoprotein" } }, "id": "IGIGPGHMMC_296", "locus": "GCA000148625_00258" }, { "type": "cds", "sequence": "contig_1", "start": 141310, "stop": 141519, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTATCACTATTTTTGGATTTTTAACATCATTAATTAACTATGACAAATATGGTATTATAGGCTCATTTATGTATATTGCATTATTTGTTTCATTCTTAACATATTTTATGTTTTTAGGAATTATAGTTAATGAAGCATATTACAAAACTTATGTGTCTTCATACACTGTTTCTAAACGTAGTATATTTGGCAAAAAAATTTAA", "aa": "MIFITIFGFLTSLINYDKYGIIGSFMYIALFVSFLTYFMFLGIIVNEAYYKTYVSSYTVSKRSIFGKKI", "aa_hexdigest": "8cfc59599130835e1b6c69564676bfe4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8060.525399999997, "isoelectric_point": 9.477190971374515 }, "id": "IGIGPGHMMC_297", "locus": "GCA000148625_00259" }, { "type": "cds", "sequence": "contig_1", "start": 141862, "stop": 142026, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTACTTTCGTTAATTCCTGGTTATCATACTTTATTTATTAATGAAGTTTTAACTCGAATTATTCCTGGTGTTACTTCTATTATTAAGATCCCTGACACCACAAAAAAAGGCGCTCAATTAACTTCTTTAATTATTCTTTGTTTGGCTTCTTTATGA", "aa": "MIVLLSLIPGYHTLFINEVLTRIIPGVTSIIKIPDTTKKGAQLTSLIILCLASL", "aa_hexdigest": "f908270ace9a5d457ffeac582491fca2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5847.109199999999, "isoelectric_point": 9.10185604095459 }, "id": "IGIGPGHMMC_298", "locus": "GCA000148625_00260" }, { "type": "cds", "sequence": "contig_1", "start": 142409, "stop": 142813, "strand": "-", "frame": 1, "gene": "engA", "product": "GTP-binding protein EngA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "nt": "ATGGTTGAATTCGAAAAGAAAATGCGTAACAAATTTCATTTTGTACCTTGGGTTCCTATTTGTTTTGTTTCAGTTAAAACAAACTCAAGAATGGACAAATTAATTGACACAATAATTAAAGTTAAACAAAATCTTGAACGTGATGTTAAACCTTCAGTTCTTTCTAACTTAATCAGAGAAACACAAATGATTCAGCCAGCTCCTCCTTATAATGGTGGCCGTTTAAATATTTATTTTGCTCGTAAAACTCAAGATCCAGTTCCTACTTTTGTTTTCTTTGTTAACAATAAAAAATATGTCCACTTTAGCTATGAAAGATTTTTAGAAAAGCAAATTAGACAAGTTTTAGACTACACTGGTTGTCCAATTAATTTAGTTTTCAAAAACAAAAGTGGTTTAGAATAA", "aa": "MVEFEKKMRNKFHFVPWVPICFVSVKTNSRMDKLIDTIIKVKQNLERDVKPSVLSNLIRETQMIQPAPPYNGGRLNIYFARKTQDPVPTFVFFVNNKKYVHFSYERFLEKQIRQVLDYTGCPINLVFKNKSGLE", "aa_hexdigest": "368c090a2b6a3155c58b38da933c87c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0QDV4", "query_cov": 0.8432835820895522, "subject_cov": 0.9912280701754386, "identity": 0.6459999999999999, "score": 159.0, "evalue": 3.45e-48, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A3B0QDV4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0QDV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "product": "GTP-binding protein EngA" }, "genes": [ "engA" ], "id": "IGIGPGHMMC_299", "locus": "GCA000148625_00261" }, { "type": "cds", "sequence": "contig_1", "start": 142841, "stop": 143311, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00350.29", "PFAM:PF02421.24" ], "nt": "GTGGATCATATTTTTCCTATTTCAGCTCAACATGGTAATGGTGTTGGTGATGTCTTAGACCAATGTCTTAAACATCTTAGACTTGATGATGAAAAGAGTGACAAACTATTCAAACTTTCAATTATTGGACGTCCTAATTCAGGTAAAAGTTCAATGTTAAATAGATTAGCAAATGAAAACCGTTCAATAGTTTCTGATATTGCGGGAACTACTAGAGATAGTGTTAAAAGTATTGTAAAAATCAAAGATGAAGTTTTTGAAATCATTGATACAGCTGGTATTACTAGAAAAAGTAAAATTGAAGATAGCGTTGACCATTATGCTTTAAAACGCGCTATGAGTTCACTTGACGAATCTAACTTATCTTTAGTTTTAATTGACTCAACTCAAGAATTATCACACTTCGATTCTAGAATTATAGGCTATGCTTTAGACAACAATAAACCAATAATTATTGTGGTTAATAAATGA", "aa": "MDHIFPISAQHGNGVGDVLDQCLKHLRLDDEKSDKLFKLSIIGRPNSGKSSMLNRLANENRSIVSDIAGTTRDSVKSIVKIKDEVFEIIDTAGITRKSKIEDSVDHYALKRAMSSLDESNLSLVLIDSTQELSHFDSRIIGYALDNNKPIIIVVNK", "aa_hexdigest": "0d3731e8754831daa3dbc867dd11e066", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00350.29", "name": "Dynamin_N", "length": 78, "aa_cov": 0.5, "hmm_cov": 0.9102564102564102, "evalue": 3.8177255660058795e-09, "score": 32.799156188964844, "start": 78, "stop": 155 }, { "id": "PF02421.24", "name": "FeoB_N", "length": 118, "aa_cov": 0.7564102564102564, "hmm_cov": 0.9491525423728814, "evalue": 3.498792190292454e-10, "score": 35.63899230957031, "start": 38, "stop": 155 } ], "seq_stats": { "molecular_weight": 17318.51749999999, "isoelectric_point": 6.193256187438965 }, "id": "IGIGPGHMMC_300", "locus": "GCA000148625_00262" }, { "type": "cds", "sequence": "contig_1", "start": 143324, "stop": 143452, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATGGTGCTTCTGACATCACTAATGACGATTTAATGATTTACAGTATGCTTCAAAAATCTGGCAAACCTATTGTCGTAGTTGCAAACAAATTAGATAATATTTCACAGTTTAATTATTCATGA", "aa": "MVDGASDITNDDLMIYSMLQKSGKPIVVVANKLDNISQFNYS", "aa_hexdigest": "20814215acf8a8b0796e5cd1a8cbeede", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4636.2374, "isoelectric_point": 4.282727241516113 }, "id": "IGIGPGHMMC_301", "locus": "GCA000148625_00263" }, { "type": "cds", "sequence": "contig_1", "start": 143573, "stop": 143716, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24" ], "nt": "ATGAGAAACAACGTTATAGCCATTATTGGAAAACCAAATGTTGGTAAAAGTACCCTATTTAATAGGCTAATTGGTAAAAGAAGTTCAATAATTTATGACCGTCCTGGAGTAACAAGGGACCGTCTTTATGAAACCTTTTCTTGA", "aa": "MRNNVIAIIGKPNVGKSTLFNRLIGKRSSIIYDRPGVTRDRLYETFS", "aa_hexdigest": "34ef83a718c1b72f31f2c9c8bf5c3e78", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3S4P1B8", "query_cov": 0.8297872340425532, "subject_cov": 0.8297872340425532, "identity": 0.615, "score": 56.6, "evalue": 8.25e-10, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A4Z0KSI3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 37, "aa_cov": 0.7872340425531915, "hmm_cov": 0.972972972972973, "evalue": 5.1546769562204115e-09, "score": 31.843690872192383, "start": 5, "stop": 41 } ], "seq_stats": { "molecular_weight": 5368.181400000001, "isoelectric_point": 10.956482505798338 }, "id": "IGIGPGHMMC_302", "locus": "GCA000148625_00264" }, { "type": "cds", "sequence": "contig_1", "start": 143720, "stop": 144028, "strand": "-", "frame": 1, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0015949", "SO:0001217", "UniRef:UniRef50_A5IZG6", "UniRef:UniRef90_C4XEA4" ], "nt": "ATGGATGGAAGAGATACTACATTCAAAATTATGCCTTATGCAGAATTAAAAATCTTTTTAACAGCAACACCAGAAGAAAGAGCAATGAGAAGATTAAACCAAAACATTGCTTTAGGATTTAATATGGACTACAACAAAGTTCTTGAAGAAGTTAAATTAAGAGACTATCAAGACATGAATCGTGAAGTAGACCCTCTTCATAAAGTTAAAGAAGCAAAAGAAATAGACTGTACAAATATGGGTTTTGAAGCAGTTGTTGTTGAAATTATTGAACTAGTTAAAAAAACAATAGAAAAACTTAATAAATAA", "aa": "MDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK", "aa_hexdigest": "d55537de57d95860022538db926cbe00", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 143720, "stop": 144328, "observations": { "mutation": [ 144244 ], "stop": [ 144244 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA4", "query_cov": 1.0, "subject_cov": 0.4473684210526316, "identity": 1.0, "score": 202.0, "evalue": 3.19e-64, "gene_start": 1, "gene_end": 102, "reference_start": 127, "reference_end": 228, "reference_sequence": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVDWNNEEEINKTLEKDIITLDERENIFLREKNVSVEIREDSISYGSSTIATYKKVRQYVVNLIQNITKKGKGYIMDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK" }, "paralog": false, "identity": 0.995049504950495, "score": 391.0, "evalue": 3.79e-141, "description": "Internal stop codon at 144244. Nonsense mutation around 144244.", "psc": { "uniref90_id": "UniRef90_C4XEA4", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_A5IZG6", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0015949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA4", "EC:2.7.4.25", "UniRef:UniRef50_A5IZG6", "GO:0004127", "GO:0005524", "GO:0015949" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZG6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZG6" ], "product": "Cytidylate kinase" } }, "id": "IGIGPGHMMC_303", "locus": "GCA000148625_00265" }, { "type": "cds", "sequence": "contig_1", "start": 144242, "stop": 144406, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13207.12", "PFAM:PF02224.24" ], "nt": "ATGACAAAAATTACAAAGATTAATATTGCAATTGATGGACCTTGCGGTGCAGGTAAATCAACTGTTTCACAAGAAGTAGCAAGAAGGCTTGGATACACATTTATTAATAGTGGAAGTGTGTATAGAGCATTAGCTTTAAATGCAAAATTATTAGGTGTTGATTGA", "aa": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVD", "aa_hexdigest": "2701fd7a256c8bd6ee28a59ae86263a8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13207.12", "name": "AAA_17", "length": 38, "aa_cov": 0.7037037037037037, "hmm_cov": 1.0, "evalue": 7.699598769995602e-09, "score": 31.983428955078125, "start": 13, "stop": 50 }, { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 40, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 1.907383583167571e-16, "score": 56.42131042480469, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5683.5617, "isoelectric_point": 9.743251991271972 }, "id": "IGIGPGHMMC_304", "locus": "GCA000148625_00266" }, { "type": "cds", "sequence": "contig_1", "start": 144495, "stop": 144725, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTAACTCAAATTATAAACTTCAAAGTGTTGATTTATATACCTTTGTAGATAGATTAGAAAAATTAATCAAAAATTCCCAATTGCCCTATGAAAAAGACAAAAAAGGAACTGAAAAAGCTGAAGAAAAATATAAAAATTTTAAAAGAAATTTTCTTGAACCTATTGACAATATGAAGCAAATTATAAATGATACAGTGCTATTAATTAATGAATACAAATAA", "aa": "MIVFNSNYKLQSVDLYTFVDRLEKLIKNSQLPYEKDKKGTEKAEEKYKNFKRNFLEPIDNMKQIINDTVLLINEYK", "aa_hexdigest": "6ac33935e39f8d85c25834f75ffd5f4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.484499999995, "isoelectric_point": 8.802593803405763 }, "id": "IGIGPGHMMC_305", "locus": "GCA000148625_00267" }, { "type": "cds", "sequence": "contig_1", "start": 145134, "stop": 145517, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAATATGAAAAAAATAATTTTGTTAGCAATCTAAAACAAAATGTCTCTATTTATACAAATAACTTTAATAAATTAGTTAAATTTATGGACAAGGATAACCACAAATTGTCATATCAATCATTTTATTATAAGGAATATGAAAAAACTAAAGAAATAACTGATTTCACATTTGAAAACTTTTTATTAAAAAACTCAAAAGCTTTTGAAGAAAAAGCATATAAATTTATGGTTAATAATGTTGATGAAAGTACTAAATTCAAAAAATATTTTGAAGACTTTACATCTACTCCTAGTGATAATACTAATTTTAATTTTTATAAAAATGTAACCTATTTGAAGAAAATAAATGATGAAATAAATTCAATTTTAAATGACTGA", "aa": "MGEYEKNNFVSNLKQNVSIYTNNFNKLVKFMDKDNHKLSYQSFYYKEYEKTKEITDFTFENFLLKNSKAFEEKAYKFMVNNVDESTKFKKYFEDFTSTPSDNTNFNFYKNVTYLKKINDEINSILND", "aa_hexdigest": "d57bb2f903f709e1ed7fd177013c66c6", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15383.972799999987, "isoelectric_point": 6.605282020568848 }, "id": "IGIGPGHMMC_306", "locus": "GCA000148625_00268" }, { "type": "cds", "sequence": "contig_1", "start": 145595, "stop": 145978, "strand": "-", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.22", "KEGG:K06178", "SO:0001217", "UniRef:UniRef50_A0A454AP59", "UniRef:UniRef90_C4XEA6" ], "nt": "ATGATTAATAAACTAACACATCCTAAATACGAAATTTTACGTACATATCGTGTTAGAATTGATTCTCCTTTAAGCTTAAAAGAATTCAAATTAATTAACGCTGGTTTGATTGTTAATGGTAAGTTTTCAAAACAAATAGTTGACCAAGTTGATACTAAAAGTTATTTAGTAACTCTTCATGTCGGCTCATACCATCACATCAAAGAATTATTCAAAGCATTAAATCATGAAGTTATTAATCTTAAAAGAGTGTCATTTGCTAACTTAACAGTTGAAAAAATGCCTGAAGGCTCATATCGAACTTTAACTCTTAAAGAAATAAAAGATCTTAAAGTTTTAATTTCATTGCAAGAAAAGAAATTATGTCAAAAAGAAGAAAATTAA", "aa": "MINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN", "aa_hexdigest": "55c819be69260b4bad3d8a552b4cfb79", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 145595, "stop": 146278, "observations": { "mutation": [ 145984 ], "stop": [ 145984 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA6", "query_cov": 1.0, "subject_cov": 0.4721189591078067, "identity": 1.0, "score": 248.0, "evalue": 3.89e-81, "gene_start": 1, "gene_end": 127, "reference_start": 143, "reference_end": 269, "reference_sequence": "MYTRTTKNLWQMNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTNDWEMINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN" }, "paralog": false, "identity": 0.9955947136563876, "score": 441.0, "evalue": 1e-159, "description": "Internal stop codon at 145984. Nonsense mutation around 145984.", "psc": { "uniref90_id": "UniRef90_C4XEA6", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.22" ], "uniref50_id": "UniRef50_A0A454AP59", "kegg_orthology_id": "K06178", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA6", "EC:5.4.99.22", "UniRef:UniRef50_A0A454AP59", "KEGG:K06178" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454AP59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454AP59" ], "product": "Pseudouridine synthase" } }, "id": "IGIGPGHMMC_307", "locus": "GCA000148625_00269" }, { "type": "cds", "sequence": "contig_1", "start": 145982, "stop": 146371, "strand": "-", "frame": 1, "gene": null, "product": "pseudouridine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "nt": "ATGAATGAAATAAATTTTTTGGTAGAAAGGTTTGCAATGAAAGAATTGATTAGGGTTCAAAAACTAATCGCACAAACAGGATATTGTTCAAGAAGAAAAGCTGAAGAATATATTGAAAAAAAACTAGTTAAAGTTAATGGAAAAATTGTTCAATTAGGTGACAAAGCAAGTGAAAACGATGAGATAAAAATTAACAATCAACTAATCTCATTACAAAAACCAAATTTTGTATATTTCCTATTAAATAAGCCTAAAAAGACAATTTCAACAGTTAAAGATCCTAAGGGCAGAAAAACAGTTGTTGATTTAATTAATTACTCAGAAAGAATTGCTCCTGTAGGTCGTTTAGACTATGATACAACTGGCGCTTTGTTATTAACAAATGATTGA", "aa": "MNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTND", "aa_hexdigest": "67f54b5998efcf146e26742f1cbe475a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FC9400C", "query_cov": 0.9147286821705426, "subject_cov": 0.9147286821705426, "identity": 0.508, "score": 112.0, "evalue": 1.1e-29, "valid": false, "product": "pseudouridine synthase", "uniref50_id": "UniRef50_UPI002FC9400C" }, "pscc": { "uniref50_id": "UniRef50_UPI002FC9400C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "product": "pseudouridine synthase" }, "genes": [], "id": "IGIGPGHMMC_308", "locus": "GCA000148625_00270" }, { "type": "cds", "sequence": "contig_1", "start": 146644, "stop": 146949, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08668.18" ], "nt": "ATGGTACCTGAAAAAGTTAACCAATATATAGGAAGTCACTTTTTATATGCTTTTGAAATAGTTCACGCTTTGCTTAGTGCAAAAAGAGCAAAACATTCAGTTTTTGCAGCAACTTTTGCTGCTGAGTTAGCTAAAACAATAAAATATGATGCAAAAGTGGCTTACTATGCTGGACTATTTCATGATATTTGTAAAGAATTAAGTGAAAATGAAAGTAGAAAATTTATTGCTCAATATGATAAAAAAGCTAACAACAAATCTATTTATCCAAATCATGTTTTACATCAAGTTTGTGGAGCTTTATGA", "aa": "MVPEKVNQYIGSHFLYAFEIVHALLSAKRAKHSVFAATFAAELAKTIKYDAKVAYYAGLFHDICKELSENESRKFIAQYDKKANNKSIYPNHVLHQVCGAL", "aa_hexdigest": "d91cccf887183e7bb44afaeda972a71e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08668.18", "name": "HDOD", "length": 62, "aa_cov": 0.6039603960396039, "hmm_cov": 1.0, "evalue": 1.1260184837425378e-06, "score": 24.255308151245117, "start": 29, "stop": 89 } ], "seq_stats": { "molecular_weight": 11435.049399999989, "isoelectric_point": 9.077486991882324 }, "id": "IGIGPGHMMC_309", "locus": "GCA000148625_00271" }, { "type": "cds", "sequence": "contig_1", "start": 147106, "stop": 147453, "strand": "-", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAAATAGGATTATTTGGTGGATCATTCAATCCAATCCATAGTGGACATATCAAAATTGCTGAATATGCCTATAAAACACTTAAATTAGACAAAATGTTTTTTATACCAACAGCGATAAGCCCATTTAAAAAGAATAATAAAGTAGCCCCTAATAAAGACAGAGTTAATATGATTAATATAGCTATTGAAAATCTAGAAGGCAATTATGCTGTGCATGATTTTGAAATCAAAAAAGGTGGCGTTAGTTATACTTTTGAAACAATTAGATATTTCAAACAACAATATCCAAATGATGAATTATATTTCTTAATAGGCAGCGATTTATTGCCAAAATTAAATAAATGA", "aa": "MKIGLFGGSFNPIHSGHIKIAEYAYKTLKLDKMFFIPTAISPFKKNNKVAPNKDRVNMINIAIENLEGNYAVHDFEIKKGGVSYTFETIRYFKQQYPNDELYFLIGSDLLPKLNK", "aa_hexdigest": "b1dec7b34b8b3b87832ee5bfd24113d9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.0, "subject_cov": 1.0267857142857142, "identity": 0.5479999999999999, "score": 123.0, "evalue": 3.13e-34, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "IGIGPGHMMC_310", "locus": "GCA000148625_00272" }, { "type": "cds", "sequence": "contig_1", "start": 148100, "stop": 148426, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11798.14" ], "nt": "GTGTTTTCAATTGATGAATGTTGGCTTGATGTAACTGATATTATCAAAAATAGAGATCCAAAAGATTTGGCTAAAAGTATCCAAAGTGACATATTAAATACTTTTGACATTCCTGCAACAATTGGAATTTCATACAACAAATTTTTAGCAAAATTATCAACTAATTTAGGTAAACCATTTGGAATTCTAGAAACAAAAGAGGAAGACATAGAAAAAAGGATTTGGCCTTTACCAATTATTGAATTTTTCGGTATTGGTGCTCCAACAGCTGAGAGATTAATTAGAATGCATATTGATACTATTGGCAAATTAGCAAAATGTAATTGA", "aa": "MFSIDECWLDVTDIIKNRDPKDLAKSIQSDILNTFDIPATIGISYNKFLAKLSTNLGKPFGILETKEEDIEKRIWPLPIIEFFGIGAPTAERLIRMHIDTIGKLAKCN", "aa_hexdigest": "6a332b7d0156303f109f8fd90ed536aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11798.14", "name": "IMS_HHH", "length": 31, "aa_cov": 0.28703703703703703, "hmm_cov": 1.0, "evalue": 6.969094496685233e-07, "score": 25.29746437072754, "start": 68, "stop": 98 } ], "seq_stats": { "molecular_weight": 12236.127199999983, "isoelectric_point": 5.35516529083252 }, "id": "IGIGPGHMMC_311", "locus": "GCA000148625_00273" }, { "type": "cds", "sequence": "contig_1", "start": 148553, "stop": 148729, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAAAACAATTTTTCATATGGATTTTGATTCATATTTTGTAAGTGCTCATCGCTCAATTAATCCTGAATTAAAAGGCAAATCAGTAGCTATTGGTAGAAGACTGAAAAGGTCAATTGTTTCTTCAGTTAGCTATGAGTTAAAAAGAAAAGGTGCTAAGGTTGGCTGA", "aa": "MENKKTIFHMDFDSYFVSAHRSINPELKGKSVAIGRRLKRSIVSSVSYELKRKGAKVG", "aa_hexdigest": "a21af93680acec1a8e2947b9dc725e8f", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6598.617200000001, "isoelectric_point": 10.345902442932129 }, "id": "IGIGPGHMMC_312", "locus": "GCA000148625_00274" }, { "type": "cds", "sequence": "contig_1", "start": 148719, "stop": 149147, "strand": "-", "frame": 3, "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "db_xrefs": [ "GO:0005198", "GO:0016226", "SO:0001217", "UniRef:UniRef50_C4XEA9", "UniRef:UniRef90_C4XEA9" ], "nt": "TTGTCTTATAATAAAGATCAAAAACGTCAAATAATAATGGAACATTATTTGAAACCTAGACACAAAAGTTCAAGTTTAAAAAACAAAATTATTAAGCACGGAGAAAGCTGTGCTGATTATTTAGAATGTGAACTAAATATAAAAAATAATCAAATTGAAGACATTAAATTTGATGGTCAAGGTTGTGCATTTTTTATAGCTTCAACTGATTTATTAATCGACTTATTAAAGGGCAAAAAAATTGACGAAAGTATCTGTTTGATAGAACTTTACGAAAAATTTATACTAGGTGAAAAATTAAATGAAAATGATATTGAAAAGCTAGGAACTTTAGCAATTTTTGATAATGTCAAAACACAATTTAATCGTGTTAATTGTGCTTTAATGTTAGCTAGAGCAATAAAGGAAAAATCTCAAAATGGAAAATAA", "aa": "MSYNKDQKRQIIMEHYLKPRHKSSSLKNKIIKHGESCADYLECELNIKNNQIEDIKFDGQGCAFFIASTDLLIDLLKGKKIDESICLIELYEKFILGEKLNENDIEKLGTLAIFDNVKTQFNRVNCALMLARAIKEKSQNGK", "aa_hexdigest": "63003fcc2aaba287440525fddb0f910f", "start_type": "TTG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_C4XEA9", "query_cov": 1.0, "subject_cov": 0.9793103448275862, "identity": 0.965, "score": 269.0, "evalue": 4.81e-91, "valid": true, "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "uniref50_id": "UniRef50_C4XEA9", "go_ids": [ "GO:0005198", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA9" ], "product": "NIF system FeS cluster assembly NifU N-terminal domain-containing protein" }, "genes": [ "sufU" ], "id": "IGIGPGHMMC_313", "locus": "GCA000148625_00275" }, { "type": "cds", "sequence": "contig_1", "start": 149137, "stop": 149559, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGTCAACAGCAGCTGATCGCTTTGAAGCTGGAACACCTAATTTAGCAGCAATTAATATGTTTAGTGTTTCAATAAACTTTTTTAATCAAATTGGTTATTGTGAAACTCAAAAAATTTTAGACGAATTAAGCTTTTATGCTTATCAAGAATTAAGCAAAATTGAAGGCATAAAACTATATAGCAATCCTAAAAATCACATTATAATATTCAATATAAAAAACTATAATAGTCATGATGTTTCACATTATTTAGGAACAAAAAATATTTATGTAAGAGCAGGTGTATTTTGTGATCATTATCTTAAAAAAATCACCAAAGAAAAGTCTTTTGTAAGAGTTTCTTTGGGTATTTATAACAACAAAAAAGATATTGATAAATTAGTTAAAGCATTAAAAGAAGGAGGTGATTTCCTTGTCTTATAA", "aa": "MSTAADRFEAGTPNLAAINMFSVSINFFNQIGYCETQKILDELSFYAYQELSKIEGIKLYSNPKNHIIIFNIKNYNSHDVSHYLGTKNIYVRAGVFCDHYLKKITKEKSFVRVSLGIYNNKKDIDKLVKALKEGGDFLVL", "aa_hexdigest": "48527182c7783e9eefff1e0812182e5b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 128, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 4.334975019441399e-29, "score": 97.58616638183594, "start": 4, "stop": 127 } ], "seq_stats": { "molecular_weight": 16013.245599999984, "isoelectric_point": 8.969631385803225 }, "id": "IGIGPGHMMC_314", "locus": "GCA000148625_00276" }, { "type": "cds", "sequence": "contig_1", "start": 149932, "stop": 150297, "strand": "-", "frame": 2, "gene": null, "product": "aminotransferase class V-fold PLP-dependent enzyme", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "nt": "ATGAAAACATTAGACAAATTAATTCAAGAACAATTCCCTTTAGCAAAAAAGATAACTTATTTGGATAGTAGTGCATTAGTTTTAAAACCAAAAATAGCTATTGATGCAGTTAACAATTTTTATTTAAATGAGTCAATTAGTACCCGTACTATTAACACTAAATTAGGAATTGAAAGCGCGCAAAAAATAAACAACTTACGTAAAAAAGTTGCTACTTTGTTGTCTTGTCAAGAACAAGAAGTTATATTTACTTCAGGAGCAACTGATTCACTAAACAAGTTTGCTTTAATGATCAAAAATCAAATTAAAAAAGGGGATATTATCCTTTTAGATGTTTATAATCATTCTTCAAATATGGCGCCTTGA", "aa": "MKTLDKLIQEQFPLAKKITYLDSSALVLKPKIAIDAVNNFYLNESISTRTINTKLGIESAQKINNLRKKVATLLSCQEQEVIFTSGATDSLNKFALMIKNQIKKGDIILLDVYNHSSNMAP", "aa_hexdigest": "937e4f7d2cd2d6c0e6e96c75bbf70f05", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028BE3002", "query_cov": 0.9421487603305785, "subject_cov": 0.8837209302325582, "identity": 0.535, "score": 118.0, "evalue": 6.14e-32, "valid": false, "product": "aminotransferase class V-fold PLP-dependent enzyme", "uniref50_id": "UniRef50_UPI0024C98C43" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C98C43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "product": "aminotransferase class V-fold PLP-dependent enzyme" }, "genes": [], "id": "IGIGPGHMMC_315", "locus": "GCA000148625_00277" }, { "type": "cds", "sequence": "contig_1", "start": 150528, "stop": 150983, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13692.12" ], "nt": "TTGGTTTGGGAAAAAAATATTAAAAAAGTTCTATTAACTTATAAAAAACTTGTTGAAGAAAATTCTAATTACCATTTAACAATGGTTGGTGGTGATATCAATTACAAGGAAATTGTTAAATATGCCTATAAAATAGGTTTAAAAGATAAAATATTGTTTACTGGCCCTATAGAAAATCAAGAAGAATTAAAAGGCATTTATTTATCTCATGATCTTTTTTTCTATCCAAGTTTATATGAAACTTATGGACTTGTTGTTAGAGAATCTGCAGCTTTAAAGTTGCCTCTCTTAGTTGTTGAAAACACAGCATGTGCCGAAAATGTGATAGATGGACAAAATAGTTTTATTTGCCAAGACGATGTTGACAATATGTACGAAAAAATTAAACAAATTTTTAAAGATAAAAATAATCTAATTAATGTGAAAAATAATATGAACAATTTATTTAATTCATGA", "aa": "MVWEKNIKKVLLTYKKLVEENSNYHLTMVGGDINYKEIVKYAYKIGLKDKILFTGPIENQEELKGIYLSHDLFFYPSLYETYGLVVRESAALKLPLLVVENTACAENVIDGQNSFICQDDVDNMYEKIKQIFKDKNNLINVKNNMNNLFNS", "aa_hexdigest": "0b68726ac7b29655a1b4aa37cd2c1a18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13692.12", "name": "Glyco_trans_1_4", "length": 135, "aa_cov": 0.8675496688741722, "hmm_cov": 0.9555555555555556, "evalue": 1.1481692660626245e-19, "score": 67.07129669189453, "start": 4, "stop": 134 } ], "seq_stats": { "molecular_weight": 17527.011300000006, "isoelectric_point": 5.728370094299317 }, "id": "IGIGPGHMMC_316", "locus": "GCA000148625_00278" }, { "type": "cds", "sequence": "contig_1", "start": 151026, "stop": 151157, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAAAAGTTGCGCAAAAAGCTTTAATTGAATATGGATATAAAAAAGAAAGTGAAATTGTACAATTAGGGACAAATTTCTTTTATCCAGAAAATGCAGATGAATTAAAAAATAAAGTTATAAAAAAATGA", "aa": "MSKVAQKALIEYGYKKESEIVQLGTNFFYPENADELKNKVIKK", "aa_hexdigest": "e5d117b08f961fae864ee84be65e4df8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4993.729100000001, "isoelectric_point": 9.004702186584474 }, "id": "IGIGPGHMMC_317", "locus": "GCA000148625_00279" }, { "type": "cds", "sequence": "contig_1", "start": 151383, "stop": 151643, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAGTGAAATTAGAGTTATTTTAATTAATGATAGTTTTACTCCTGTAATAGATGGAGTTGTCAGGGTAATGCAAAATTATGCTTCTAATTTTATTAAAAAAAATGTTAAAGTTCTGGTTTTGGCTCCAGAATACAAAAAAATGAAATTAGATGAAGATAAAGGTCTTAATTATTCTGTCATTAGAATACCTAGACAATTAGCTAAAATTGGTGGTTATGAAGTAATGAACACTATTTTGAGTAGAAAAATTTGA", "aa": "MTNSEIRVILINDSFTPVIDGVVRVMQNYASNFIKKNVKVLVLAPEYKKMKLDEDKGLNYSVIRIPRQLAKIGGYEVMNTILSRKI", "aa_hexdigest": "91b8d0591ea5591d0999f147bee4d1b0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9842.572999999995, "isoelectric_point": 9.791796684265137 }, "id": "IGIGPGHMMC_318", "locus": "GCA000148625_00280" }, { "type": "cds", "sequence": "contig_1", "start": 151729, "stop": 151950, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAAAACCAAATTATCAAAAAGCTCTTGAGACTTTGTATGAACCAAATTCAAATATTCATCATGTAATTAATTGGCCTTTTAAAAAGGCTACAAAAATTAACATATATGAAAATATCAATTTTTTTGATACTATTGATGTTAAATTTGAAGATATAAAAATTCGGGTGCCAAAAAATTATGAAACAATATTTTTACAAAACTATGGTCCAAATTGA", "aa": "MFKKPNYQKALETLYEPNSNIHHVINWPFKKATKINIYENINFFDTIDVKFEDIKIRVPKNYETIFLQNYGPN", "aa_hexdigest": "0595a260e0fd2fc1e42b5d7c9cb9dc8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8838.021799999995, "isoelectric_point": 8.89278507232666 }, "id": "IGIGPGHMMC_319", "locus": "GCA000148625_00281" }, { "type": "cds", "sequence": "contig_1", "start": 151990, "stop": 152160, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCCCTAAATTTTCAAAAGATAAAGATTCTCAAAAAGATGCAACTTTTTTAGATCTTTTTATCGATATACCAACAACTAAAAAAAGAATAAAAAAATATTTATCTCTTAAAAATAAATTGGGTTATTTGAACGCTTATTCTCAAAGAAAGTTTTTTGATATTCAATGA", "aa": "MFPKFSKDKDSQKDATFLDLFIDIPTTKKRIKKYLSLKNKLGYLNAYSQRKFFDIQ", "aa_hexdigest": "eaa71bb4324b8f9b5be28cbbbe10b908", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6723.792000000001, "isoelectric_point": 9.8733491897583 }, "id": "IGIGPGHMMC_320", "locus": "GCA000148625_00282" }, { "type": "cds", "sequence": "contig_1", "start": 152263, "stop": 152397, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04991.19" ], "nt": "ATGAATGAAAAACAAACTAAAGTTTATGAACTGTTGAAAGAGTTTATTGAAATTTGTAATAAACACAATTTAGATTATTGTGCTATGTATGGAACAGCATTAGGAGCAATAAGACACAATGGATTTATACCTTGA", "aa": "MNEKQTKVYELLKEFIEICNKHNLDYCAMYGTALGAIRHNGFIP", "aa_hexdigest": "d5025df6f2ee5b016ad3728226234bea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04991.19", "name": "LicD", "length": 26, "aa_cov": 0.5909090909090909, "hmm_cov": 1.0, "evalue": 2.9577843295062217e-09, "score": 33.315032958984375, "start": 19, "stop": 44 } ], "seq_stats": { "molecular_weight": 5117.8992, "isoelectric_point": 6.6983842849731445 }, "id": "IGIGPGHMMC_321", "locus": "GCA000148625_00283" }, { "type": "cds", "sequence": "contig_1", "start": 152474, "stop": 153463, "strand": "-", "frame": 1, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "EC:1.1.1.399", "KEGG:K25634", "RefSeq:WP_013354411.1", "SO:0001217", "UniParc:UPI0001E32FBF", "UniRef:UniRef100_UPI0001E32FBF", "UniRef:UniRef50_A0A3P8K9L4", "UniRef:UniRef90_C4XEB3" ], "nt": "ATGAAAATTATTACTTTTAAAGTTAGAAATGTTGAAAAACCAATATTTGAAGAAATTAACAAAAAATTTGGTTATAAACTTGAAATTCATAGTGAAAGTTTAACTATGGAAAATGTCAATTTAACAAAAGGTTTTGACGCTGTTATTGTTAGAGTTGATGGTTCAATAAATAAAGAAATGTTAGATGCATTAAAAGCAAATGGCATTAAATATCTTTTAACAAGAACTGTCGGAACAGACCATATTGATTTAGAATATGCTCATAAATTAGGCTTTAAAATGGCTAGAGTTCCTTCATATTCACCAACAGCAATTGCTGAATTAGCTTTTGCTCATGCTTTAATGTTATCAAGACAAACACTTCATTTTGGACATAAAGGTTTTAACAAAGACTTTACTATTGATCCTGAAGGTTTTGCGACTGAATTAAAAAATAAAACAGTTGGAATAGTTGGGGTTGGAAAAATAGGACTTGCAGCTGCTAAAATGTTTAAAGTTATGACAAACAATGTTTTAGGCTTCGATTTATATCCTTCAGAAGAAGGCAAAAAAGTTGTTAAATATACAAAAACTCTAGAAGAATTAGTTAAAAAATCAGATATCATTTCTTTCCATTGCCCCTATATTAAAGGTAAAAACGACAAAATGATTAATGATGCATTAATTAAGCAAATGAAAAAAGGTGTAATTATTGTTAACACAGCTAGAGGCCAAATTCAAGATGAAGAAGCATTATTAAAAGGTCTAAAAAGTGGCAAAATTCAAGCTATTGCTACAGATGTTTTGAACAATGAAAAATCAATTTTCTTTAAAAAGCATGCAGAAATAGAAAACAAAACATTTCGCCAATTATTAGAATTCTATCCTCGTTTTGTTTTAACACCTCACATTGGTTCTTATACTGATGAAGCTGTTAAAAACATGGTTGAATACACTTATGACAATCTAAAAGAATATATCGAAACTGATCAATGCAAAAATGCAATTTAA", "aa": "MKIITFKVRNVEKPIFEEINKKFGYKLEIHSESLTMENVNLTKGFDAVIVRVDGSINKEMLDALKANGIKYLLTRTVGTDHIDLEYAHKLGFKMARVPSYSPTAIAELAFAHALMLSRQTLHFGHKGFNKDFTIDPEGFATELKNKTVGIVGVGKIGLAAAKMFKVMTNNVLGFDLYPSEEGKKVVKYTKTLEELVKKSDIISFHCPYIKGKNDKMINDALIKQMKKGVIIVNTARGQIQDEEALLKGLKSGKIQAIATDVLNNEKSIFFKKHAEIENKTFRQLLEFYPRFVLTPHIGSYTDEAVKNMVEYTYDNLKEYIETDQCKNAI", "aa_hexdigest": "7a2e1acf6a3f6a9e3d10d8bf0a46daa1", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32FBF", "ncbi_nrp_id": "WP_013354411.1", "uniref100_id": "UniRef100_UPI0001E32FBF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FBF", "RefSeq:WP_013354411.1", "UniRef:UniRef100_UPI0001E32FBF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FBF", "uniref90_id": "UniRef90_C4XEB3" }, "psc": { "uniref90_id": "UniRef90_C4XEB3", "product": "D-lactate dehydrogenase", "ec_ids": [ "1.1.1.399" ], "uniref50_id": "UniRef50_A0A3P8K9L4", "kegg_orthology_id": "K25634" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K9L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K9L4" ], "product": "D-lactate dehydrogenase" }, "genes": [], "id": "IGIGPGHMMC_322", "locus": "GCA000148625_00284" }, { "type": "cds", "sequence": "contig_1", "start": 153502, "stop": 153636, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTTTTGCAGCTACACCACCTGCTACAGTTGCTGTTGGTTTTGCAATTAGTGAAAATAAATGTGCTAGATTAGCAAGCTCTGCTTCAGCATTTAGTACATTACTTGCAGTTATCTTTTTACCTATTTGA", "aa": "MVIFAATPPATVAVGFAISENKCARLASSASAFSTLLAVIFLPI", "aa_hexdigest": "c24c58320307d048a84b3244beda3903", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4468.282899999997, "isoelectric_point": 7.975980186462403 }, "id": "IGIGPGHMMC_323", "locus": "GCA000148625_00285" }, { "type": "cds", "sequence": "contig_1", "start": 153883, "stop": 154083, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTCAATTAATACCAGGTGCGGGAGCAACATATAAAGATTTTAGTCAAACTATTAAAGTTAATGGAAGTGATGTAGTTATTAAATATTCAGCTACTTTTGGTGAAAACTTTAAAACAGTTTTAACACCAGACAAAAAAGTATTCTATATGTTAAATGCAAATAATGAATATATTAAAACCACTAAACCAGTTGGTTGA", "aa": "MTQLIPGAGATYKDFSQTIKVNGSDVVIKYSATFGENFKTVLTPDKKVFYMLNANNEYIKTTKPVG", "aa_hexdigest": "50ed93513abc707265b99383acb57bab", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7295.301300000002, "isoelectric_point": 9.302352714538571 }, "id": "IGIGPGHMMC_324", "locus": "GCA000148625_00286" }, { "type": "cds", "sequence": "contig_1", "start": 154084, "stop": 154191, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATGAAAGGTTTAAAATTCAACAAAGAAAATATTAAAGCTTCAGCAAAATCAATGTTTTTAAATCCAATTATTATTGCAACTTTTGCTGGATTAATTCTTTGA", "aa": "MQMKGLKFNKENIKASAKSMFLNPIIIATFAGLIL", "aa_hexdigest": "78cfbf956a516b43818a46747f703483", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3882.7434999999987, "isoelectric_point": 10.176350593566895 }, "id": "IGIGPGHMMC_325", "locus": "GCA000148625_00287" }, { "type": "cds", "sequence": "contig_1", "start": 154327, "stop": 154440, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCATAAAACACCAGAATCAGTGGAAAATGCTCAAGAAAACACTATTATTGGTGAAATGAGTGAAACAAAAACAGTTGATGAATCTCGTCATCGTGCAATGGTTGTTTGA", "aa": "MPHKTPESVENAQENTIIGEMSETKTVDESRHRAMVV", "aa_hexdigest": "10755b970deafaacb484efd14149e65c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4182.627900000001, "isoelectric_point": 5.086316871643065 }, "id": "IGIGPGHMMC_326", "locus": "GCA000148625_00288" }, { "type": "cds", "sequence": "contig_1", "start": 154456, "stop": 154587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTGCTTTACCAGCTTTAGCACTCAAAGGCTTTATGAGCGATATTAGTCTTGGACTTCTTCAAGAACAAGGTATTATCTTAGGAATTGCATTTGGATTCTATATCTTGCTTTCATTAGTGGCTTGA", "aa": "MKVALPALALKGFMSDISLGLLQEQGIILGIAFGFYILLSLVA", "aa_hexdigest": "68ad2e2e4a7845b60a4de1f7e9b59846", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4536.525399999999, "isoelectric_point": 5.8228364944458 }, "id": "IGIGPGHMMC_327", "locus": "GCA000148625_00289" }, { "type": "cds", "sequence": "contig_1", "start": 155143, "stop": 155394, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGCTATTGATCCTTTTGTTTCTATATTCTTTAGATATGTTGTTTCATTGATTGTTTTAGGTAGCATTGTTTTACCGTCAGCAAAAATCTATGGAAAAATGGGTGAATTATTTGCTAAAGCAAACATTTTATTAGTTGTTGCTTCTGGAATTGTGGGTTGCATTTCATATATGACATTCTATTATGCACTTGATAAAATAGGTGTTCCAATGGCTACAGGATTAAATATTTCCTATGTTGGTTGA", "aa": "MKNAIDPFVSIFFRYVVSLIVLGSIVLPSAKIYGKMGELFAKANILLVVASGIVGCISYMTFYYALDKIGVPMATGLNISYVG", "aa_hexdigest": "49ffab95b7eb90d8aa65fdaaf05462af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8944.691199999996, "isoelectric_point": 9.16742038726807 }, "id": "IGIGPGHMMC_328", "locus": "GCA000148625_00290" }, { "type": "cds", "sequence": "contig_1", "start": 155461, "stop": 155679, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTATGACAATGTATGTCCTTGGTATTGTTTATGTCGGTACTGGAATAAGTAGTGCAATTACAGTTTGTTATCCTATTGTTGGCGCAATGCTTGCTTTTATCTTTTTGAAACAAAAAAGTACAAAAAATGGTTTATTCGGATCAATAATTTCACTTTTATGTATTTTAACTTTAGGAATTTTACAATTTAATAAAGACGATATTAAAAATGGATGA", "aa": "MGMTMYVLGIVYVGTGISSAITVCYPIVGAMLAFIFLKQKSTKNGLFGSIISLLCILTLGILQFNKDDIKNG", "aa_hexdigest": "70bea4cea61b95a6e60019c829bdbc86", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7707.268399999993, "isoelectric_point": 9.099599647521973 }, "id": "IGIGPGHMMC_329", "locus": "GCA000148625_00291" }, { "type": "cds", "sequence": "contig_1", "start": 155920, "stop": 156030, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATAAAAATTCAAATGTAGAAGTTAATGAAATTCAAAACGATAAAAAAGTTAAAAATAAAAATTTATTTAATAAATTTGTTGCTTTATTTTCAGGGATTATGTGA", "aa": "MNDKNSNVEVNEIQNDKKVKNKNLFNKFVALFSGIM", "aa_hexdigest": "f5070d8a45990f166b5e2f6e68fd5c90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4171.753500000001, "isoelectric_point": 9.303642082214353 }, "id": "IGIGPGHMMC_330", "locus": "GCA000148625_00292" }, { "type": "cds", "sequence": "contig_1", "start": 156159, "stop": 156368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGA", "aa": "MYNLFFSSLVGFLCKYVSKSQLASLTPYSLSMHIPAKSKNGSLLAAYSQSINLILLSLFIKIFPEVESI", "aa_hexdigest": "1399650964bfb56fc1c382f6599735c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7664.005399999998, "isoelectric_point": 9.25226078033447 }, "id": "IGIGPGHMMC_331", "locus": "GCA000148625_00293" }, { "type": "cds", "sequence": "contig_1", "start": 156302, "stop": 156577, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAGCTTTAAATAGTTATCGCAAACTACATAATCAAAATTTATCAGTTGATTTTGAATTTGATATTCTAAAAATTTTGGACTATTACATTTCAATAATTGGAGAAAAATACAAAACATTTCCAAAACATTTATTAGACAATGAAGCTAAAATTAGAAAAATGCTTGCTTATCATAAAAGTAAAAATTATCCAAAAGTTTTATGTCATATTGATTCAACTTCAGGAAATATCTTAATAAATAAGGATAATAAAATTAAATTAATTGATTGA", "aa": "MELALNSYRKLHNQNLSVDFEFDILKILDYYISIIGEKYKTFPKHLLDNEAKIRKMLAYHKSKNYPKVLCHIDSTSGNILINKDNKIKLID", "aa_hexdigest": "bb817008b4542126b4d277adeea13be7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10741.422199999985, "isoelectric_point": 9.162972068786619 }, "id": "IGIGPGHMMC_332", "locus": "GCA000148625_00294" }, { "type": "cds", "sequence": "contig_1", "start": 156620, "stop": 157027, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATCTTAAAAGATATAGAGCTTAAGAAATACAATTGTGACCAAAAGGTCTATGAAATAGACGATTTCAAAGATTTATTGACATTTAATAGAAAAGCTCCTTGCTTTATAAACGATGTTTATTTAAACAAAATTAAAGAAGTTTTTAATGCCAAATTTGAAGATATTTTAGATATTCAACCAGTTAAAAAAGGAATGACTAATAACTCATTTTCTTTTAAAATAAAAGATAAAAAATATATTGCTAGAATACCAAAAGATGGCTCAAAGAGTTTAATTAATAGATACCAAGAAGAAAAAATTTATCAAAAACTAAAAGGCAATGAATTAGTTGAAAATTTAATTTATTTTGAAACCAAAAATAATATTGATGATCCTTTACATGGTTTTAAAATAACTGAATGA", "aa": "MDILKDIELKKYNCDQKVYEIDDFKDLLTFNRKAPCFINDVYLNKIKEVFNAKFEDILDIQPVKKGMTNNSFSFKIKDKKYIARIPKDGSKSLINRYQEEKIYQKLKGNELVENLIYFETKNNIDDPLHGFKITE", "aa_hexdigest": "5b961e929c2f8c8d42b547d59ec90c57", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16068.357999999991, "isoelectric_point": 8.37097797393799 }, "id": "IGIGPGHMMC_333", "locus": "GCA000148625_00295" }, { "type": "cds", "sequence": "contig_1", "start": 157451, "stop": 157639, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAAATACTCAAATAATAATTTTAGCTGCTGGCTTTGGTTCAAGACTAACGCCTCTTTCACTTAAGACGCCAAAAGGATTATTAGAAATTAAAAAAGATTTAAAAATTGTTCCTCATCTTTTATCTTATTTAAAAGATTATAAAAATAAGACAATAGTAACTGGCTACAATTATCAATCTTGA", "aa": "MDKNTQIIILAAGFGSRLTPLSLKTPKGLLEIKKDLKIVPHLLSYLKDYKNKTIVTGYNYQS", "aa_hexdigest": "e210dcd2e2b6ed5bc32040c1362c3f38", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6993.214900000004, "isoelectric_point": 9.795277976989745 }, "id": "IGIGPGHMMC_334", "locus": "GCA000148625_00296" }, { "type": "cds", "sequence": "contig_1", "start": 157983, "stop": 158267, "strand": "+", "frame": 3, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAA", "aa": "MGDYVIVVNAEKVILTAKKEEDKVYYHHSGYPGGLKSITAAKLRAKKPTALVEKAIYGMLPHTKLGNKQRKNLSVVAGPDHKHQAQKPESLEVR", "aa_hexdigest": "029cc29247d3ecb5cd02735fdeba0c5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.755, "score": 145.0, "evalue": 2.95e-43, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "IGIGPGHMMC_335", "locus": "GCA000148625_00297" }, { "type": "cds", "sequence": "contig_1", "start": 158269, "stop": 158688, "strand": "+", "frame": 1, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_013354416.1", "SO:0001217", "UniParc:UPI0001A8C0DC", "UniRef:UniRef100_C4XEB8", "UniRef:UniRef50_Q8R7Y9", "UniRef:UniRef90_C4XEB8" ], "nt": "ATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAA", "aa": "MATAKKVTKKEVVEYRGLGRRKSSTARVILRPGKGKFTINKREALAYLTSNLYIQDAEQPLVLTETKGQFDITVNVSGGGLSGQAGAIRLGIARALLAASDTYREKLKPAGMLTRDARVTERKKPGLNKARRARQFSKR", "aa_hexdigest": "6865a3611ec67d39576e69dc1c2da313", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C0DC", "ncbi_nrp_id": "WP_013354416.1", "uniref100_id": "UniRef100_C4XEB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0DC", "RefSeq:WP_013354416.1", "UniRef:UniRef100_C4XEB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEB8", "uniref90_id": "UniRef90_C4XEB8" }, "psc": { "uniref90_id": "UniRef90_C4XEB8", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q8R7Y9", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q8R7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8R7Y9" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "IGIGPGHMMC_336", "locus": "GCA000148625_00298" }, { "type": "tRNA", "sequence": "contig_1", "start": 158745, "stop": 158821, "strand": "+", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 82.2, "nt": "GCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 158779, 158781 ], "id": "IGIGPGHMMC_5", "locus": "GCA000148625_00299" }, { "type": "cds", "sequence": "contig_1", "start": 158973, "stop": 159083, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGA", "aa": "MKKDQLPYLPKNLKRRQQKSFELLKVLDKICRENNL", "aa_hexdigest": "00fb306c34e80ce74b54c09c2d458fbd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4429.2621, "isoelectric_point": 10.01279430389404 }, "id": "IGIGPGHMMC_337", "locus": "GCA000148625_00300" }, { "type": "cds", "sequence": "contig_1", "start": 159531, "stop": 159680, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGA", "aa": "MYYITNWPNKRIFHQVTLTLEQIENGMQDIEMNGHYFKSLKENESVLER", "aa_hexdigest": "6b8a7f6965eeab0e6562a144fc821096", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5989.726199999998, "isoelectric_point": 5.503856086730956 }, "id": "IGIGPGHMMC_338", "locus": "GCA000148625_00301" }, { "type": "tRNA", "sequence": "contig_1", "start": 159863, "stop": 159946, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 67.2, "nt": "GCCCAAGTGGTGGAATGGTAGACGCTGTGGACTCAAAATCCACTGGAGAGATCCGTGCAGGTTCAAGTCCTGTCTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 159941, 159943 ], "id": "IGIGPGHMMC_33", "locus": "GCA000148625_00302" }, { "type": "rRNA", "sequence": "contig_1", "start": 160020, "stop": 160126, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8991596638655462, "score": 69.1, "evalue": 4.3e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TCCAGTGGTTATAGCGAAGGGGACCCACCTGATTCCATTCCGAACTCAGTAGTTAAGCCCTTTAGTGCCGACGATACCAGAGATGGGAAAATAGGGAGCTGCTATTT", "id": "IGIGPGHMMC_40", "locus": "GCA000148625_00303" }, { "type": "cds", "sequence": "contig_1", "start": 160487, "stop": 160720, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGA", "aa": "MPIKISGFIFGPLIGGIVGLISDLLSFLFVPSFYNPIYTVAAMVDGIISGLVGLLFIKLFGYYFGGEFQDNVHENRI", "aa_hexdigest": "82836e21f88382467fca2d6588f01146", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8433.870399999996, "isoelectric_point": 4.8263353347778315 }, "id": "IGIGPGHMMC_339", "locus": "GCA000148625_00304" }, { "type": "cds", "sequence": "contig_1", "start": 160994, "stop": 161224, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGA", "aa": "MVSGYTLMFFFIIIARFKMKPSNYLVIVPIVIFSALIELINVPLLSFADQQSLQKGEDNNIFVFVFNHILFSPVKI", "aa_hexdigest": "004f4485996cecc998465410b5c0913e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8741.412499999995, "isoelectric_point": 8.120389366149901 }, "id": "IGIGPGHMMC_340", "locus": "GCA000148625_00305" }, { "type": "cds", "sequence": "contig_1", "start": 162468, "stop": 162584, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGA", "aa": "MKTMLSIALEYISEKSKDGKNIKFNDIFKEIKANLNDK", "aa_hexdigest": "15155089558628a0506e499f424d0955", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4419.124800000002, "isoelectric_point": 9.100760078430177 }, "id": "IGIGPGHMMC_341", "locus": "GCA000148625_00306" }, { "type": "cds", "sequence": "contig_1", "start": 162761, "stop": 162850, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGA", "aa": "MKLVNASSMLKKAKKGKYAVPHININNLE", "aa_hexdigest": "63c020b77579d9ad77c92bd5bc852cc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3240.8828000000003, "isoelectric_point": 10.12516269683838 }, "id": "IGIGPGHMMC_342", "locus": "GCA000148625_00307" }, { "type": "cds", "sequence": "contig_1", "start": 162929, "stop": 163315, "strand": "+", "frame": 2, "gene": null, "product": "class II fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "nt": "ATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGA", "aa": "MGGMNIIVGMINGMMKDYNITVPVALHLDHGTYEGAKKAIKAGYTSLMFDGSHLPYEENLAKTKELVKLAKKVNMSMEAEVGTIGGEEDGIIGAGEFADPAEAKEMAETGIDVLAAGIGNIHGPYPIS", "aa_hexdigest": "b8ece84c05e30ae815d3fcafb7083b00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0015C42C51", "query_cov": 0.9921875, "subject_cov": 0.8523489932885906, "identity": 0.504, "score": 132.0, "evalue": 4.97e-37, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_UPI0035C6842C", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_UPI0035C6842C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "product": "class II fructose-bisphosphate aldolase" }, "genes": [], "id": "IGIGPGHMMC_343", "locus": "GCA000148625_00308" }, { "type": "cds", "sequence": "contig_1", "start": 163851, "stop": 164054, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "TTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGA", "aa": "MYFTNDTIKQGVRFFGIDTPEIFKGKNNQHLALLENYYGQKAKNYLYNLTYQKNIKIKYITKDKYNR", "aa_hexdigest": "39ea9fe130d16a562402197c5ffa17dd", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 56, "aa_cov": 0.835820895522388, "hmm_cov": 0.8928571428571429, "evalue": 1.1491612692966802e-09, "score": 34.69647979736328, "start": 12, "stop": 67 } ], "seq_stats": { "molecular_weight": 8120.213899999999, "isoelectric_point": 9.698124122619632 }, "id": "IGIGPGHMMC_344", "locus": "GCA000148625_00309" }, { "type": "cds", "sequence": "contig_1", "start": 164469, "stop": 165404, "strand": "-", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_S5M1G4", "UniRef:UniRef90_A0A3P8K9P9" ], "nt": "ATGGCTCAAGACTTAGTTGTTGGAAACCAAGTTCTCTTTGACGATGGTAAATTATCAACAATTGTAACTAAAGTTGATAGAAAAGCTGGTTATGTTGTTGTTAAAACACTTAACAAGCACTTATTAAAAACTAACAAACGTATTAATTTACCAGGTGTTGACTTTTCTCTTCCATTTTTAGCTCAAAAAGACATTGATGATGTTAAATTTGGTATTAAAAACGGAATTAACTATGTAGCAGCATCATTTGTTAACTCATCTAAAAATGTCCAAGAATTACGTAAATTATTAGATGACAATGGTGGAAAACACGTTAAAATCATTTCTAAAATTGAAAGCCACTTAGGAACCAAAAACATTGATGAAATCATTCAAGCATCAGATGGTGTTATGGTTGCTCGTGGGGACTTAGGTCTTGAAATTCCTTACTATGATGTTCCTTATTTTGAAAAAATAATGATCAGAAAATGTCGTGAAGCGGGTAAACCAGTTGTTGTTGCAACTCAAATGTTAGACTCAATGGAAAACTCACCACAACCTACACGTGCTGAAGTTACTGACGTTTACTATGCAACAGAAATGGGTGCTGACTCAACAATGCTTTCAGGTGAAAGTGCATCAGGCAACTATCCTTTAGAAGCAGTTACAGTTATGTCAAAAATTAACAAACGTGCTGAAAGTGAATTTTATGACAAAAATTACTATGATGTTCAATTAGAAGAAATTAGAAAAAATTCTTCAAATTCAAAACGTTCAAGAATTGCTTATGACGTTGCAAACAGAACACGTTATGGTGACTATAGATTTGCTGTTGTTCTTTCAAGAACAGGTCAATTATTAAGTGAAGTTGCTAAATTTAGACCAAATACATTAATCGTTGGTATTTTAAATGATGAAAACCTAATCGGTGGATTTGGTGTTACTTCTAGTGTTTGA", "aa": "MAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSV", "aa_hexdigest": "1f4f1a05519381d88e406f276f439efe", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 164325, "stop": 165704, "observations": { "mutation": [ 164471, 165470 ], "stop": [ 164471, 165470 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K9P9", "query_cov": 1.0, "subject_cov": 0.6519916142557652, "identity": 1.0, "score": 601.0, "evalue": 2.09e-214, "gene_start": 1, "gene_end": 311, "reference_start": 119, "reference_end": 429, "reference_sequence": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKVWTTKEKYETVEGTEKELTVAYDMAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSVWVSLDSIELFAKIKKDKSKALNALKPYGVKKGDRFLVVENESITEFVA" }, "paralog": false, "identity": 0.9956427015250545, "score": 888.0, "evalue": 0.0, "description": "Internal stop codon at 164471, 165470. Nonsense mutation around 164471, 165470.", "psc": { "uniref90_id": "UniRef90_A0A3P8K9P9", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_S5M1G4", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K9P9", "EC:2.7.1.40", "UniRef:UniRef50_S5M1G4", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_S5M1G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5M1G4" ], "product": "Pyruvate kinase" } }, "id": "IGIGPGHMMC_345", "locus": "GCA000148625_00310" }, { "type": "cds", "sequence": "contig_1", "start": 165468, "stop": 165758, "strand": "-", "frame": 3, "gene": null, "product": "pyruvate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "nt": "ATGAACATTACTGACAAAAGAAGTAAGTTAGTTATTACAGTTGGTCCATCAAGTGATAACTACAAAATGCTTCGTTCATTAATTGAAGCAGGTGCAACCTGTGTTCGTGCTAACTTTAGCCACGGAGACCATGCAGAACAATTAAACAAATTTGTTCTTGCACAAAAAATTTCTAAAGATTTACATCTTCCTATATCTCTTATGTTAGATACTAAGGGTCCGGAAATTCGTTTAGGAAAAATGAAAGATGGAAAACAAGAAATTCCAGCAAACAAAGAAATTAAAGTTTGA", "aa": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKV", "aa_hexdigest": "d00046a4307e35aa0ac1a254290185b4", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387B60A9", "query_cov": 0.8645833333333334, "subject_cov": 0.9540229885057471, "identity": 0.711, "score": 125.0, "evalue": 8.67e-36, "valid": false, "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_UPI00387B60A9", "kegg_orthology_id": "K00873" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B60A9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "product": "pyruvate kinase" }, "genes": [], "id": "IGIGPGHMMC_346", "locus": "GCA000148625_00311" }, { "type": "cds", "sequence": "contig_1", "start": 165942, "stop": 166190, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTAAAGATGATTTTAAAAATAGTCACCTTTACCAATTATTCTATTGTACAAAACAAACAGAAGATTATGATAAAGACAAAACAAAATATAAAAATGAAGGTTTAGTTTCATTTGATCCAGCAAAAGGCGAAGCTACCTTTGCAATTGGTATTAGAGTTAAAAATTCAAATAATAATTATAAGTCTTCTTCAATTCCTGAATATATTGGACATATAACTTTAGGTGGTTTTAAAAAATCATAA", "aa": "MKFKDDFKNSHLYQLFYCTKQTEDYDKDKTKYKNEGLVSFDPAKGEATFAIGIRVKNSNNNYKSSSIPEYIGHITLGGFKKS", "aa_hexdigest": "ba99a65ba617ad9e85d4b70fba226503", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9440.507699999998, "isoelectric_point": 9.16303653717041 }, "id": "IGIGPGHMMC_347", "locus": "GCA000148625_00312" }, { "type": "cds", "sequence": "contig_1", "start": 166260, "stop": 166457, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAGGACAAAAATGATAAAACATCTTTAATAAAATCAAGAATGATTGACATAAAAAATCCTATTGTTCAAGAAATAGATATTAATTATTTTGAACTTGAAAAAGTATTGAAAAATCATGTTGAAATATCTATTTTAGGTAACGATGAAATTAAAAATATTGACTTTAAAACACCTTCAACTTCACTTGAATGA", "aa": "MPKDKNDKTSLIKSRMIDIKNPIVQEIDINYFELEKVLKNHVEISILGNDEIKNIDFKTPSTSLE", "aa_hexdigest": "6b95fea2f551b33ff42349dbf176ccfc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7544.609099999998, "isoelectric_point": 5.330781364440917 }, "id": "IGIGPGHMMC_348", "locus": "GCA000148625_00313" }, { "type": "cds", "sequence": "contig_1", "start": 166602, "stop": 166757, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTAGAGATAAAATAAGACAAACTTTTTACAAGAAAGAAAAGAATAGTCTTGATGAATTACTTGCTGCATTACCACAAAAAGATCTTTCAAGTGATTTAAACAAAAATAAAGAAGAAATTTTAAAAAATAATTCAACAATTGAAAGTTGA", "aa": "MDIRDKIRQTFYKKEKNSLDELLAALPQKDLSSDLNKNKEEILKNNSTIES", "aa_hexdigest": "cc208832ae65abf5bcf0d1b621151380", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5966.6853999999985, "isoelectric_point": 6.175920295715332 }, "id": "IGIGPGHMMC_349", "locus": "GCA000148625_00314" }, { "type": "cds", "sequence": "contig_1", "start": 166857, "stop": 167057, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAATCAATTATTTTCATTAAGAAATAATCGTTTTGATAGAATTGACAAATTTTATAAAGATTACAATATTTTCTACCATTCATATGCAAATGATTTTACAGGTACACTCTATTTGACTGTTTCATTAGAAGATAAAGCTAATGAAAAAGATAAAGATAAAAAAACAAGAAACATTAGTGATAGAATAAAAAACTGA", "aa": "MRNQLFSLRNNRFDRIDKFYKDYNIFYHSYANDFTGTLYLTVSLEDKANEKDKDKKTRNISDRIKN", "aa_hexdigest": "70cab7cf96435a1a43b11fa1e643cbbd", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.936699999999, "isoelectric_point": 9.474483299255372 }, "id": "IGIGPGHMMC_350", "locus": "GCA000148625_00315" }, { "type": "cds", "sequence": "contig_1", "start": 167127, "stop": 167423, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCGTAAATTATCATTAACATTAGGAACATTAGCAACAGGTGTTGTTGCAGTTGGAATTGGTACGGCTATTTATGCTTTAGTTAAAACTAATAAAGACAATAAAAGTATTGAAAGACAGCTAAGCTCTTTTACTTTTTCTCTTGCAAAACCTAAAGACAAATTATACCAAACTAATATTGCAAATCACTTAGCAAGTGAATTTACTTACTCAAATTATTACTATTTTGAAACATACAATAGCAACAAAGAACCAACTTTGAAAAACACAAAAGAATATGAAAAAAATTATTGA", "aa": "MNRKLSLTLGTLATGVVAVGIGTAIYALVKTNKDNKSIERQLSSFTFSLAKPKDKLYQTNIANHLASEFTYSNYYYFETYNSNKEPTLKNTKEYEKNY", "aa_hexdigest": "a5046db0ae9ce25ddb62516bcc737bcb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11177.487099999991, "isoelectric_point": 9.397894859313965 }, "id": "IGIGPGHMMC_351", "locus": "GCA000148625_00316" }, { "type": "cds", "sequence": "contig_1", "start": 167678, "stop": 167809, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTTGCGGTTAAAGATTTTTTAAAAAATGAACCTTCTCCAAAACATATCTTTTTGCTTATAGATAGTCGAACAAGATTTACTAAAAATGATTTAGAAACCTTGACATTTTTAAATTCATTAAACTGA", "aa": "MNLAVKDFLKNEPSPKHIFLLIDSRTRFTKNDLETLTFLNSLN", "aa_hexdigest": "9a7b4fa6c0f92d811c625176db386597", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5035.772399999999, "isoelectric_point": 8.211225318908692 }, "id": "IGIGPGHMMC_352", "locus": "GCA000148625_00317" }, { "type": "cds", "sequence": "contig_1", "start": 168070, "stop": 168498, "strand": "-", "frame": 2, "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "db_xrefs": [ "EC:2.7.7.3", "GO:0004595", "GO:0015937", "KEGG:K00954", "RefSeq:WP_013354425.1", "SO:0001217", "UniParc:UPI0001E32FCA", "UniRef:UniRef100_UPI0001E32FCA", "UniRef:UniRef50_A0A449A8S0", "UniRef:UniRef90_C4XEC9" ], "nt": "ATGATTAAAAAAGCAATTTTTCCAGGATCTTTTGATCCTATACACAAAGGTCATATATCAGTAATTGAAAAAGCACTAAAGCTTTTTGATGAGCTTATAGTAATAGTTTCAATTAACCCTGATAAAGACAATTTAGGTAATTTAGAAGAAAGATATCAATCAGTCAAAAAAACCTTAAAAGACTATAAAACAGTCACTGTGGTAAAAAATGAAAATGATTTGATAGCTCAAATTGCTAAAGAAAAAAATGTAAAGTTCCTAGTTAGATCAGCCAGAAATAATTTAGATTATGACTATGAGCTAGTACTAGCAGCTGCTAATCATCACTTAAATTCTGAGCTTGAAACTATACTTATAGTACCAGACTATGAAAACATAGACTATTCTTCAACTCTTTTAAGACATAAACAAAAATTAGGTAAAAAATAA", "aa": "MIKKAIFPGSFDPIHKGHISVIEKALKLFDELIVIVSINPDKDNLGNLEERYQSVKKTLKDYKTVTVVKNENDLIAQIAKEKNVKFLVRSARNNLDYDYELVLAAANHHLNSELETILIVPDYENIDYSSTLLRHKQKLGKK", "aa_hexdigest": "d4bfea261e1c0e579ccd19d4eea76d5c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32FCA", "ncbi_nrp_id": "WP_013354425.1", "uniref100_id": "UniRef100_UPI0001E32FCA", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FCA", "RefSeq:WP_013354425.1", "UniRef:UniRef100_UPI0001E32FCA" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FCA", "uniref90_id": "UniRef90_C4XEC9" }, "psc": { "uniref90_id": "UniRef90_C4XEC9", "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "ec_ids": [ "2.7.7.3" ], "uniref50_id": "UniRef50_A0A449A8S0", "kegg_orthology_id": "K00954", "go_ids": [ "GO:0004595", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A8S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A8S0" ], "product": "Phosphopantetheine adenylyltransferase" }, "genes": [ "coaD" ], "id": "IGIGPGHMMC_353", "locus": "GCA000148625_00318" }, { "type": "cds", "sequence": "contig_1", "start": 168501, "stop": 169019, "strand": "-", "frame": 3, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef90_C4XED0" ], "nt": "ATGGGACTTACACCTTTAGCAGGAATTATGATGGGATCTCGTTCAGGCGATATTGATCCTTCAATTCATGAATTTATTAATGAACAACTTGGTTTAGATATTCACCAAATCACAGAAGCTCTTAACAAGAAAAGTGGGCTTTTAGGAGTTTCAGGTGTTTCAAGCGATATGCGTGATGTATGTAAAGCAGCAGACGAAGGCAATAAACGTGCTATTTTCACACTTGAATTGTATGCACAAAAAATAGCTGATTTTACCGCTAATTATGCAAACAAAATTGGCAACAAATTAGATGCTATTGTTTTCACTGCTGGTGTTGGTGAAAACACTCCTTCAATGCGTCAAATGGTAGTTGATAAACTACATTTTGCAAAGATTAAATTAGATGACAAAAAGAATAATTCTAAAATTGAAGATTATGCTTTAATTTCTACAAAAGATAGCGAAATCAAAGTATATGTAATTAGAACAAATGAAGAATTATTAATTGCAAAACATGCAATAAATTTGTATAAATAA", "aa": "MGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK", "aa_hexdigest": "43a499d540fc49d4941e831af02a5074", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6YLS3", "query_cov": 0.9651162790697675, "subject_cov": 0.8383838383838383, "identity": 0.518, "score": 145.0, "evalue": 5.34e-41, "valid": false, "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_R6YLS3", "kegg_orthology_id": "K00925" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 168501, "stop": 169319, "observations": { "mutation": [ 169193 ], "stop": [ 169193 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.4321608040201005, "identity": 1.0, "score": 333.0, "evalue": 2.17e-112, "gene_start": 1, "gene_end": 172, "reference_start": 227, "reference_end": 398, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9926470588235294, "score": 531.0, "evalue": 2.1e-192, "description": "Internal stop codon at 169193. Nonsense mutation around 169193.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "IGIGPGHMMC_354", "locus": "GCA000148625_00319" }, { "type": "cds", "sequence": "contig_1", "start": 169191, "stop": 169694, "strand": "-", "frame": 3, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef50_UPI0028BDD23C", "UniRef:UniRef90_C4XED0" ], "nt": "ATGAGTAAGCAAAAAGTATTAGTTATTAATGCGGGAAGTAGTTCAATAAAGTTATCACTTTTTGATAAAGCTTCTTTAGAAATTATTGCAAGTGGTATTGCAGAGAGGATTACCTTATTAGAAGGTAAAATAACAATCAAATATAACGGTCAAAAATTTAACCGTGATGTAACAATGCCTAACCACACTGTGGCTGTTGAAAACATTTTAGAATTAATGTCACAAATTGGTTTAATTGACAATCCAAAAGAAATCGAATTAATTGGCTTTAGAGTTGTTCATGGTGGACCTTACTTTACAACAACAGTTAAATTGAATACTAAAGAAATTGATTTAATTGAAAAAGCTAAAATTTATGCACCACTTCACAATCCAGGTGCAGTTCAAGCCATGAAAGCATTTAAAAAAGTTATGCCACATGCCAAAATGAGTGTTGACTTTGATACAGCATTCCACACAACAATTCCAGATGTCAATGCAATTTATCCAATTCCATATCAATGA", "aa": "MSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQ", "aa_hexdigest": "b3d35d81c65fe847b2a9740e420e5e5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021AE5BBA", "query_cov": 0.9640718562874252, "subject_cov": 0.8256410256410256, "identity": 0.609, "score": 192.0, "evalue": 9.47e-60, "valid": false, "product": "Acetokinase family", "uniref50_id": "UniRef50_UPI0028BDD23C" }, "pscc": { "uniref50_id": "UniRef50_UPI0028BDD23C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028BDD23C" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 168888, "stop": 169697, "observations": { "mutation": [ 169193 ], "stop": [ 169193 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.41959798994974873, "identity": 1.0, "score": 327.0, "evalue": 9.51e-110, "gene_start": 1, "gene_end": 167, "reference_start": 2, "reference_end": 168, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9888475836431226, "score": 522.0, "evalue": 3.84e-189, "description": "Internal stop codon at 169193. Nonsense mutation around 169193.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "IGIGPGHMMC_355", "locus": "GCA000148625_00320" }, { "type": "cds", "sequence": "contig_1", "start": 169704, "stop": 170657, "strand": "-", "frame": 3, "gene": null, "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354427.1", "SO:0001217", "UniParc:UPI0001E32FCC", "UniRef:UniRef100_UPI0001E32FCC", "UniRef:UniRef50_Q4A5B1", "UniRef:UniRef90_C4XED1" ], "nt": "ATGAAATTTACATCTTTAATTGAAAAAAGAATTAAAGCAATGAAAACTAAAAAAAGAATTGCTTTTATAGATGGTGATGATAGTAGAGCACTAGATACCATTAAACAATTAGAACAATTTCCTAATGTTGAATTAACTTTATTAGTTGAAACTGATGTTAAGTCAAAAACCAATACTAAGTTTATAAATATTAATAAAGATAAACAAAAACAAAAAGATTTAGCTCAAAAATATTTTGAATTAAGAAAAGGCAAAGAAACAATTGAACAAGCTCAAAGAAACCTAAAAACAAGACCTTTCTATGCTATGATGCTTTTAGCTACAGGTGAAGTTGATGGAGTAGTTGGCGGACTTAATTATGCAACTGCAGATATCTTAAGAGCAGCTTTTAAAGCTATAGGCGCTAGAGAAGGAGTAAAAACTATTTCTTCAGTTATGGTAGTACATAACGAAAAAAATACTTACTTCTTCAGTGACATTTCAGTAAATGTTAAGCCTGATCAAAAAGCTTTAGTAGAAATTGGACTTAATGCTGCTCAATTTGCTCAAAGCATGGGGATAGAACCTAAAATAGCCTTCTTAAGTTTCTCAACTAACTATTCAGCAAAAACACCTGAAAGTGAATTAGTACACAATGCTGTTGAAGAATTCAATACCATTTATCAAGGTACTAAAGCTATTGGAGAAATTCAATTAGATGCTGCTTTAGATTTAGAAGTTAGAAAAGCTAAATATAAATTAGATTACTATAAAGAACCAGCAAATACTTTAATTTTCCCTGATTTAGATGCTGGAAATATAGGCTATAAATTAGCTCAAAGATTAGGAAATTATGGGGCAATTGGACCTATTGTTGTTGGTACTAAAAAACCAGTCAATGATCTTTCAAGAGGTTCAAGTGTAAAAGATGTAATTAATACAATTTTAATCACTGCATTGCAGGCAGAGAAATAG", "aa": "MKFTSLIEKRIKAMKTKKRIAFIDGDDSRALDTIKQLEQFPNVELTLLVETDVKSKTNTKFININKDKQKQKDLAQKYFELRKGKETIEQAQRNLKTRPFYAMMLLATGEVDGVVGGLNYATADILRAAFKAIGAREGVKTISSVMVVHNEKNTYFFSDISVNVKPDQKALVEIGLNAAQFAQSMGIEPKIAFLSFSTNYSAKTPESELVHNAVEEFNTIYQGTKAIGEIQLDAALDLEVRKAKYKLDYYKEPANTLIFPDLDAGNIGYKLAQRLGNYGAIGPIVVGTKKPVNDLSRGSSVKDVINTILITALQAEK", "aa_hexdigest": "839b9eb02ae2dc440297c139a5a524a6", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32FCC", "ncbi_nrp_id": "WP_013354427.1", "uniref100_id": "UniRef100_UPI0001E32FCC", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FCC", "RefSeq:WP_013354427.1", "UniRef:UniRef100_UPI0001E32FCC" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FCC", "uniref90_id": "UniRef90_C4XED1" }, "psc": { "uniref90_id": "UniRef90_C4XED1", "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "uniref50_id": "UniRef50_Q4A5B1" }, "pscc": { "uniref50_id": "UniRef50_Q4A5B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5B1" ], "product": "Phosphate acetyltransferase" }, "genes": [], "id": "IGIGPGHMMC_356", "locus": "GCA000148625_00321" }, { "type": "cds", "sequence": "contig_1", "start": 171166, "stop": 171372, "strand": "+", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013354428.1", "SO:0001217", "UniParc:UPI0001E32FCD", "UniRef:UniRef100_UPI0001E32FCD", "UniRef:UniRef50_C4XED3", "UniRef:UniRef90_C4XED3" ], "nt": "ATGACAAGAGTTCAACTTGAAATCTTAATAACTGTTATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCGATTAAAACAAAGAAAAAAAGATTAA", "aa": "MTRVQLEILITVISYKEILERNNSKDLDEAIEKFKKRYGFSVTIKQIIAILGIKKSTYYYRLKQRKKD", "aa_hexdigest": "aaa72a4f088b52c89bc2fc1bdd9a3160", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001E32FCD", "ncbi_nrp_id": "WP_013354428.1", "uniref100_id": "UniRef100_UPI0001E32FCD", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FCD", "RefSeq:WP_013354428.1", "UniRef:UniRef100_UPI0001E32FCD" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FCD", "uniref90_id": "UniRef90_C4XED3" }, "psc": { "uniref90_id": "UniRef90_C4XED3", "product": "Transposase", "uniref50_id": "UniRef50_C4XED3" }, "pscc": { "uniref50_id": "UniRef50_C4XED3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED3" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_357", "locus": "GCA000148625_00322" }, { "type": "cds", "sequence": "contig_1", "start": 171773, "stop": 171922, "strand": "+", "frame": 2, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "nt": "ATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGA", "aa": "MSRVGNSLYNREIEYFFSILKTEIFPNFCKRVKLLTFNELKNEIKEFID", "aa_hexdigest": "80df6f3186f2c7c96547632095695c59", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFF27A6", "query_cov": 1.0, "subject_cov": 0.8448275862068966, "identity": 0.6940000000000001, "score": 70.5, "evalue": 3.7e-15, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0009993DF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0009993DF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "product": "IS3 family transposase" }, "genes": [], "id": "IGIGPGHMMC_358", "locus": "GCA000148625_00323" }, { "type": "cds", "sequence": "contig_1", "start": 172272, "stop": 172961, "strand": "-", "frame": 3, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "nt": "ATGAAAATCTTAGATGGAAAAACTCTTGCTCAAAAAAGAACTAAAGAACTCATTGCACAATTTAAGGATATAAAGGAAAAGTTAGGCAGAAAACCAATTTTATCAATTATTCAAGTTGGCGATAATCCCGCATCTACTCACTACATCAAAAACAAATTAAAGAAGGCTGAAGAATTAGGAGTAGAAGCAATTCATCATCATTTTCCAGCTGATATTAGACAAAAAGATTTGCTTAAGAAATTAGACATTATTAATGAAAAATCTGATGGTATTGTTATTCAACTTCCTTTACCTAAAGAATTTGCCACACAAGTTATTTTAGATAGCGTTCGCATTGAAAAGGATATTGATGGTTTATGTACCAGAAATGCATTCAATTTCTACAATCAAGCGGATGACTTTACCTTAGTTCCAGCTACTGCTATTGGTATTTTAGATTTATTAAATAGCTACAATATCAAAATGATTGACAAAAAAGCTGCTGTTATTGGTCGTTCAAGTTTAGTTGGAAAACCTACAGCATTTCTATTAAAACAAGAAGGCTGCAATGTTTCTACCTACAATAGAAACACAGGCATTAAAGGTGTTGAAAATGCTGATATCATTGTAGTTGCTGCTGGTGAAGCCAAACTTCTTAAAAAAGAAAACATTAAAGAAGGTGCTGTTGTAGTTGATGTTGGATCAAACTGA", "aa": "MKILDGKTLAQKRTKELIAQFKDIKEKLGRKPILSIIQVGDNPASTHYIKNKLKKAEELGVEAIHHHFPADIRQKDLLKKLDIINEKSDGIVIQLPLPKEFATQVILDSVRIEKDIDGLCTRNAFNFYNQADDFTLVPATAIGILDLLNSYNIKMIDKKAAVIGRSSLVGKPTAFLLKQEGCNVSTYNRNTGIKGVENADIIVVAAGEAKLLKKENIKEGAVVVDVGSN", "aa_hexdigest": "b0ba4cc88f8b47363debf5a24bf6dbee", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8L7E0", "query_cov": 1.0, "subject_cov": 0.8063380281690141, "identity": 0.873, "score": 403.0, "evalue": 1.38e-140, "valid": false, "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_L0RXG1" }, "pscc": { "uniref50_id": "UniRef50_L0RXG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "IGIGPGHMMC_359", "locus": "GCA000148625_00324" }, { "type": "cds", "sequence": "contig_1", "start": 172958, "stop": 173533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19", "PFAM:PF10458.14" ], "nt": "ATGCCAAATAAAAAAGCTGCTTTAGATAACCTAAAATCATTACTTTTAATAGCTCATCCATTTTTACCTTTTATTACTGATCACTTATATAAAGTACTATTTAACAAAGAATTATTAGCTCAAGAATTTCCAAACTTAAAGAATCAAAAAGTTGAAAATCTTGAACAAATTGAACAAATAATTTCTATTGTAAATATACTTAGAAAATATCGTGAAGACAATAATATTAGTAAAAAAGAAGTGCTTTATTATGACACTAATATTAAACAAAATGAGTTTGTTAAAAATTCACTAAAATCACTATCAAACAGTGAAATTAGTGAAAATAAAGACACTTTATTTGTTGATGGAAATATTAATGTTTATATTAAAGAATCAGCCGAGCAAAAAAACACTTACATTGCTAAATTAAAAGAGAAAATAGCTAATTTAGAAAGCGAAGTTAAACGTTCAAACAATATTTTAAGTAATCAAAACTTTATAGCTAAAGCACCTACATCTAAAGTTGAAGAAGAAAAACAAAAACTAGCTAAATATCAAGAAGAATTAGACAAATACAAGGAGGAGTTAAAATGA", "aa": "MPNKKAALDNLKSLLLIAHPFLPFITDHLYKVLFNKELLAQEFPNLKNQKVENLEQIEQIISIVNILRKYREDNNISKKEVLYYDTNIKQNEFVKNSLKSLSNSEISENKDTLFVDGNINVYIKESAEQKNTYIAKLKEKIANLESEVKRSNNILSNQNFIAKAPTSKVEEEKQKLAKYQEELDKYKEELK", "aa_hexdigest": "11acec314f31c8c1d10b522c436ddeb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 78, "aa_cov": 0.3769633507853403, "hmm_cov": 0.9871794871794872, "evalue": 2.6068824487928415e-14, "score": 49.35884475708008, "start": 9, "stop": 80 }, { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 58, "aa_cov": 0.3036649214659686, "hmm_cov": 1.0, "evalue": 7.292507715250108e-13, "score": 44.672828674316406, "start": 134, "stop": 191 } ], "seq_stats": { "molecular_weight": 22322.269500000017, "isoelectric_point": 8.473611640930176 }, "id": "IGIGPGHMMC_360", "locus": "GCA000148625_00325" }, { "type": "cds", "sequence": "contig_1", "start": 173831, "stop": 173995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTTTTTGGATTAGAATTCATGAAACAAAAACCTTTTGATAGAGTATTAATTCATGGTCTTGTTAGAGACCAATTTGGTAAAAAAATGTCTAAATCATCAAACAATGGTATTGATCCAATTCAAGTTATAAATGAAAATGGTTCTGATGCTTTAAGATGA", "aa": "MYFFGLEFMKQKPFDRVLIHGLVRDQFGKKMSKSSNNGIDPIQVINENGSDALR", "aa_hexdigest": "75a39d5d0be6991b7f97c1c8ac695a9c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.5784339334092615e-18, "score": 62.66581726074219, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 6218.106800000001, "isoelectric_point": 9.300805473327635 }, "id": "IGIGPGHMMC_361", "locus": "GCA000148625_00326" }, { "type": "cds", "sequence": "contig_1", "start": 174269, "stop": 174364, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAATTAGCTCAAGATATTTTAGACCACTTAAAATCAAAAGATGCAGTTAACTTTTATCCAAAAAGATTTGTTAATGTTATCAAAAAATGA", "aa": "MDKLAQDILDHLKSKDAVNFYPKRFVNVIKK", "aa_hexdigest": "76a673df5861ddef108e6deeff302e9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3675.3042000000005, "isoelectric_point": 9.601679420471193 }, "id": "IGIGPGHMMC_362", "locus": "GCA000148625_00327" }, { "type": "cds", "sequence": "contig_1", "start": 174374, "stop": 174856, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ], "nt": "ATGTACTATATCAAGTATCCAATCTTGAATTCTAATAAATATTTAACAGTTGCCACAGTTAGAACTGAAACTATGCTTAGTGACGTTGCTGTTATTTACAATCCAAAAGACAAAAGATATAAAGATTTTAAAGACATTCAAATTGTTCATCCTTTAACTAAACAAATAATTCCATTTGTGCCTGATGAATATGCAGACATGAAATTTGGTAGTGGCTTAATGAAATTATCAGCTCATGCTGAAGTTGATATTGACATTATCAAAAAACACAAATTACAAATTAATGAAACTATTGATAAAGACGGTTTAATTAACTGTCCAAAAAGTCAATTCCACAAATTAACTCGTCAAGAAGCAAGAGAAAAAATTGGTCAATATTTAGCTGATAATAATTTATTAGTTAAAGTAGAAACCATTACTTCAAATGTTGGTTATTCAGAACGTTCAAATACTCCAGTTGAAACTCTTGTTATGCCACAATGA", "aa": "MYYIKYPILNSNKYLTVATVRTETMLSDVAVIYNPKDKRYKDFKDIQIVHPLTKQIIPFVPDEYADMKFGSGLMKLSAHAEVDIDIIKKHKLQINETIDKDGLINCPKSQFHKLTRQEAREKIGQYLADNNLLVKVETITSNVGYSERSNTPVETLVMPQ", "aa_hexdigest": "4df4a5302e1b8becaf4a3335513dd746", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF093D7", "query_cov": 0.84375, "subject_cov": 0.8709677419354839, "identity": 0.593, "score": 161.0, "evalue": 5.81e-48, "valid": false, "uniref50_id": "UniRef50_UPI002FF093D7" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF093D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18421.091699999994, "isoelectric_point": 8.502171134948732 }, "id": "IGIGPGHMMC_363", "locus": "GCA000148625_00328" }, { "type": "cds", "sequence": "contig_1", "start": 175100, "stop": 175210, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGATCATGCCGGTATCGCAACTCAAAGCAAAGTTGAAAATACAATTTATCAAAAAGAAGGATTAAACAGACATGATTTAGGAAGAGAGAAATTTCTTAAACGTGTTTGA", "aa": "MDHAGIATQSKVENTIYQKEGLNRHDLGREKFLKRV", "aa_hexdigest": "4580192047fb739f6a76c75b47c368e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 1.1177478084207314e-07, "score": 26.926347732543945, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 4183.7084, "isoelectric_point": 9.396476554870606 }, "id": "IGIGPGHMMC_364", "locus": "GCA000148625_00329" }, { "type": "cds", "sequence": "contig_1", "start": 175630, "stop": 175833, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTTCTGATTCATTGGTTGAATCACAAGAGTTTGTGAAAACAAATACAAAAAATCAAAAGCATTTAAGACGGAACTTAATATGCTTATTGAACTTAAGAAATAGACCATTTTTAAAGTTGGTTTATTTTTTAAAACAATTGTTTTATAGTGATTTTGAAAAATTACAGGGATTAAAAATGGATGATAAAAAGATCTAA", "aa": "MEFSDSLVESQEFVKTNTKNQKHLRRNLICLLNLRNRPFLKLVYFLKQLFYSDFEKLQGLKMDDKKI", "aa_hexdigest": "b9db24f72d10da17a8ecc2e9be35428a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8154.490499999994, "isoelectric_point": 9.609931373596194 }, "id": "IGIGPGHMMC_365", "locus": "GCA000148625_00330" }, { "type": "cds", "sequence": "contig_1", "start": 175820, "stop": 175963, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCATCGTCAAGAGATTTCATTTGTTAAAATGTTTGAAAATATTTTACATATTAACGGAAGCAAAAATTATTATGTAGCTACATTGTTATATTTAATATTTAATTTTGTTTTAGGTTATGCAATTCATGGAATTTTCTGA", "aa": "MSHRQEISFVKMFENILHINGSKNYYVATLLYLIFNFVLGYAIHGIF", "aa_hexdigest": "5c39bea7bddd000749e6b6aa04520755", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5553.4569999999985, "isoelectric_point": 8.157845497131351 }, "id": "IGIGPGHMMC_366", "locus": "GCA000148625_00331" }, { "type": "cds", "sequence": "contig_1", "start": 176498, "stop": 176689, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACACAAATTCAATATTTTTTGTATAATTTTAAGCATGGATACAATGTCAAAATTTTATTTAAAATTTAATACTGAATTTGCAAATGGAAGACCAGAAGCTTTTAGCGCATTCCACTTGACATTTTTAGCAACATTTCTTATTTTAGGCATCATTTTATGTGTTGCTTTAAGAAATAGAAAAGAATGA", "aa": "MGHKFNIFCIILSMDTMSKFYLKFNTEFANGRPEAFSAFHLTFLATFLILGIILCVALRNRKE", "aa_hexdigest": "73c5e07f6cf408f0ac09f2c2f8fe65fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7292.634699999997, "isoelectric_point": 9.236852836608886 }, "id": "IGIGPGHMMC_367", "locus": "GCA000148625_00332" }, { "type": "cds", "sequence": "contig_1", "start": 176767, "stop": 177075, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGTTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATACTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCGAGAAAATATGATGAATTTATTGATGTTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGACACAAGAGTTTGA", "aa": "MAEAKKKTTAANKAKKPVATKTTKKVTAAKTIKKPVATKAKVAKKPTLDKYYISEKPIKKTGDIEFNFKKAKQKTSRKYDEFIDVLKEFIFVSETSKNDTRV", "aa_hexdigest": "e3fcd1a82c91d0fd2700836e4941cb7f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11528.465999999991, "isoelectric_point": 10.115041160583491 }, "id": "IGIGPGHMMC_368", "locus": "GCA000148625_00333" }, { "type": "cds", "sequence": "contig_1", "start": 177322, "stop": 177672, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTGTTGAAGAAATAATTACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGA", "aa": "MLASEVQKDDIVASTPTRGCEVFVTNIYPSNGQMEVEYVVKNENNQSDSQIKTLYGFKQNPVVEEIITISDEEEKKLESNIVEDTEPAEVVDVESDENKNTSVENDKKSKMSKELL", "aa_hexdigest": "0704c7a5a60cbe477a5bb608d0a88a15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13075.268799999994, "isoelectric_point": 4.27033634185791 }, "id": "IGIGPGHMMC_369", "locus": "GCA000148625_00334" }, { "type": "cds", "sequence": "contig_1", "start": 177900, "stop": 178322, "strand": "-", "frame": 3, "gene": null, "product": "Integral membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9", "UniRef:UniRef90_C4XED9" ], "nt": "TTGATTAATCAAAACAAAAATCTAAATAAAATTTTGATCGATTTTGTTTCATTTACTAGCCTTTATGGCGGAATTTCAATAATGATATTTATTCCAAATGAAGTCTTAAATAAATCGATATTTTTTGATTGTCACTCAATGATTCAACATGGAATTTTAATGTTAATTAGTATTGTTTTAATTTTTAATAATTATTCAAAATTCGACAAAGGAAATTACATTATTCATAATCTTTTTATGTTTCTAATAATGTTTAGTGTAGTAGTAATATTGAATGAAATTTTTTATCAAACAGCACACAAAGACTTAAATAAATTACAAGAAAATTATCCAAATTTATTAGCAATTAGTCATCGTCTTAATAATCATTTGACACTATTATTTGAGAAAATTTTTAGTGTTAAATTAAATGGCAACTACTGA", "aa": "MINQNKNLNKILIDFVSFTSLYGGISIMIFIPNEVLNKSIFFDCHSMIQHGILMLISIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHRLNNHLTLLFEKIFSVKLNGNY", "aa_hexdigest": "04df91a03a19c793fa6b38ef8730a5c9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 177774, "stop": 178415, "observations": { "mutation": [ 177902, 178379, 178397 ], "stop": [ 177902, 178379, 178397 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED9", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 0.971, "score": 267.0, "evalue": 2.5e-89, "gene_start": 1, "gene_end": 140, "reference_start": 32, "reference_end": 171, "reference_sequence": "MDKDNVWYSKYKWSYFPLFFCSIPYFLFPIYLINQNKNLNKILIDFVSFTSLYGGISIMIFIPNEVLNKSIFFDCHSMIHHGILMLIGIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHHLNNHLTLLFEKIFSVKLNGNYWVITLLYPVINFVLALAVYSLIIIIKVSNKIPENKNKMSFQ" }, "paralog": false, "identity": 0.9624413145539906, "score": 403.0, "evalue": 2.46e-145, "description": "Internal stop codon at 177902, 178379, 178397. Nonsense mutation around 177902, 178379, 178397.", "psc": { "uniref90_id": "UniRef90_C4XED9", "product": "Integral membrane protein", "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED9", "UniRef:UniRef50_C4XED9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9" ], "product": "Integral membrane protein" } }, "id": "IGIGPGHMMC_370", "locus": "GCA000148625_00335" }, { "type": "cds", "sequence": "contig_1", "start": 178704, "stop": 178979, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGCCATAAAAATGAAAATAATTCTTTATTAGTAACTGATGAATTGAGTCAAGAAAAAACTATTGAAACACAAATAAATGATTTAGAGAATCCTTTATTAGAAAAGACAATTATGATAGCTGCTTTCCAGCCTAATAAAATTAAAAATAACAAAAATTTGGATAAAACATTTACTCAAGAATTAGAAGCTATTCAAAAAGTAAATAATAGTAATCAAAAAGTTGAAACTGACTTGACAATGACTCAAGTATTATCAGATGACATATATTAA", "aa": "MQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY", "aa_hexdigest": "f1d9a50958cafc651587f3115446cffd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10449.592199999994, "isoelectric_point": 4.6114839553833 }, "id": "IGIGPGHMMC_371", "locus": "GCA000148625_00336" }, { "type": "cds", "sequence": "contig_1", "start": 178980, "stop": 179573, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGACTTTGTATTTAATAATGTTATTGTTCTTAAAAATAGTTTAGTTTTAGCTAAAAAAGTTTCTAATAAAATTCTTCACAAATTAGACCAATTTGACAGATTAATTGAAATTTTTGAAAAATCATCAATCAATACAAAGAGCTTAATTAGACCTTATGAAAATATTTCAAAAATTAACAAACTAAAAATGAACTTTATTTCTTCTATTATTAAGAAACCAAAACTAATTATTATTGCTGATGATCCTGATATTAAAGATATAAAACTTAAATTTGAATTTTTAAGAGCTGCTTTTGAATTATGTGAACAAAGTAAAATAAATATTATTTTTGTTACTCAAGATAAAGAATTAGTTGAAGACAATGAGTTTGACTATATCTATTTGATTGCTAATAATAAAGAAGTTGAATTTGGAGATTTAAAAGAAGTTTTAAATAACACAATTAACCCTGAAATTAAAAGTAAAATTAATGACCAAGCCATTTCACAAGAAATTATTAGAAGTCAAAAAGAATTTGTTTTTAGTGATATTTATGATGTAGATGAAGAGCACTACATTATTACACCTTCAAAATATTTCAAATTATGA", "aa": "MFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDIKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKL", "aa_hexdigest": "3cc4ca22abeebbd16493bd85491266c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23190.631800000006, "isoelectric_point": 5.581043434143066 }, "id": "IGIGPGHMMC_372", "locus": "GCA000148625_00337" }, { "type": "cds", "sequence": "contig_1", "start": 179805, "stop": 180887, "strand": "-", "frame": 3, "gene": null, "product": "MAG1360 family OppF-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97", "UniRef:UniRef90_C4XEE0" ], "nt": "ATGGCTGAAAAAGAAGAACAAAATTTACAATATCCTTTAGTTTACAAATATGAAGAACTTAAAAAAAATTACGGTGAAAACAAGGAACGTATTAACCAATTAAATAAGACAGTTAATAAATACTATTATCCTGTTCGTCATGTTATTTTGAATATTTTAGTAGAACAAGCTAACTCATTAATGGCGATTTTTCGTGATTTTGAAAGAAACTTCAATAGTTTTTTAAATAAAACAACAAAAAATATTGCTGAATATAACTTTGATGAATTACAAACAAGATTTAAAGAAATTGAAAGCTACCAAGACTTATTTTTTGGTGAACAAATTACTTTAATATTAGACTTGTTTTCTCAAGTATTTAATAAATTTGAATTCTTTTATAATGCATCTAAATCAAATATTGACTACTTGGGTGATGAACAAATAAATCACTTAAAAAAGAGACTTTACTACTTAACCAAAATGAGAAAAACTTCTAAAAAAAGAGTGGAAGACAACTTAAAAATTAGAGACTTAAAACATGATATTTGTGCTTTAGATGAAATAGCAAAAGAAATTTGTCACAACTCAGATCAAAAAATTAAATACTTTGCTTTTAGATATAAAAGAAGTGCCGAAATTGATTTGTATAAATCAACTTTTTTTGCTAAAGAAAGCTCACAAAGACAAATTTTAAACAAAAGATATTTTGTTAAAATGTTTGCTCACAAGTTACTTAAAAAATATAAAAAATATTTTAAATATTTAAAAATTGATGAACTTGATGAAATGAGAAGAACACTAGATACCACCATCAAATTATTTATTAGCAATAGTTTAGGAAATTTAACTTACAGCAATAAAAACTTTTCAATTAAAAAAATTAAAAAGATTCTTAAAGAAGAATTCTACTTTAATGTCGAAAGTTATAAAATCAAATCAAAAAGCAATTTTGACTTAATTAAAGATTCAAAAGATCAAATTCAAAGTCAAATAATGTCTATTAAAAATAGATCATATGGAACATACTTCAATACTGTCCCTGAAAGATCTATCAAAGTTGCAAAAGAAATTTTAACTAATGCTCAAAGTGAACATAATTGA", "aa": "MAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHN", "aa_hexdigest": "60d9e0ab51f4738d19f5480ecea7d40e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 179502, "stop": 181187, "observations": { "mutation": [ 179672, 179807, 180944 ], "stop": [ 179672, 179807, 180944 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE0", "query_cov": 1.0, "subject_cov": 0.43373493975903615, "identity": 1.0, "score": 659.0, "evalue": 6.07e-231, "gene_start": 1, "gene_end": 360, "reference_start": 104, "reference_end": 463, "reference_sequence": "MLFINMKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNYWNIEFNKNNNAFSEINFFNMAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHNWKFNKIQSEFYNTLKSNQKLLNSYDTMTLINSKIKRKIMKLKTSLWFKRIFNRSINEKDKLNIKNFSANLKKIIEKFEMFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDTKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKLWMQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY" }, "paralog": false, "identity": 0.9875222816399287, "score": 1049.0, "evalue": 0.0, "description": "Internal stop codon at 179672, 179807, 180944. Nonsense mutation around 179672, 179807, 180944.", "psc": { "uniref90_id": "UniRef90_C4XEE0", "product": "MAG1360 family OppF-related protein", "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE0", "UniRef:UniRef50_A0A3P8LI97" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97" ], "product": "ABC transporter ATP-binding protein" } }, "id": "IGIGPGHMMC_373", "locus": "GCA000148625_00338" }, { "type": "cds", "sequence": "contig_1", "start": 180942, "stop": 181181, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAATAAAATATTTGGAATTGAAAATTGTTTTAAGAATCTAAATTTTGAAGATGATTATGTAGATTTACAAAAAATTGAGATTTACGATCAACTTAAAACTGCAATTTTAGTTACTGATAAAAACAGAAACCTAATTGGTTCCAATTTTTACAATACAATTTTCAATAACAATTTGAACATTTTATCTTTCTTTTCTAAGAATAATAAAAAAGGTTCAGGAACAAATTATTGA", "aa": "MKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNY", "aa_hexdigest": "7fde6c4fb76b82243f2d789bc966aacc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9251.382599999994, "isoelectric_point": 9.068719291687014 }, "id": "IGIGPGHMMC_374", "locus": "GCA000148625_00339" }, { "type": "tmRNA", "sequence": "contig_1", "start": 181248, "stop": 181625, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 181331, "stop": 181420, "aa": "AEDKKAEEVNISSLMIAQKMQSQSNLAFA", "nt": "GCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAG" }, "nt": "GGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGATATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCA", "id": "IGIGPGHMMC_37", "locus": "GCA000148625_00340" }, { "type": "cds", "sequence": "contig_1", "start": 181754, "stop": 181855, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGA", "aa": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNF", "aa_hexdigest": "6d44cb23e3ab1d5550544ec4465c6797", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3692.2652000000007, "isoelectric_point": 8.140632438659669 }, "id": "IGIGPGHMMC_375", "locus": "GCA000148625_00341" }, { "type": "cds", "sequence": "contig_1", "start": 182030, "stop": 183130, "strand": "+", "frame": 2, "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "KEGG:K03601", "SO:0001217", "UniRef:UniRef50_C4XEE1", "UniRef:UniRef90_C4XEE1" ], "nt": "ATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGCTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAAGAATTAGTTAAAAGCAGCAAAGAATTATTCTTACAAAAAATACAAGCAAAATTTGAAATGACTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAA", "aa": "MNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIAESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFKELVKSSKELFLQKIQAKFEMTKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK", "aa_hexdigest": "5c79344b4f866d19eff90eb93da2ab4e", "start_type": "ATG", "rbs_motif": null, "genes": [ "xseA" ], "truncated": "5-prime", "pseudogene": { "start": 181754, "stop": 183130, "observations": { "mutation": [ 181853, 181865, 181955 ], "stop": [ 181853, 181865, 181955 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE1", "query_cov": 1.0, "subject_cov": 0.7991266375545851, "identity": 0.995, "score": 622.0, "evalue": 5.79e-222, "gene_start": 1, "gene_end": 366, "reference_start": 93, "reference_end": 458, "reference_sequence": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNFWQTYWIKASISKLSIKNHAYLNIVDGEYSSNAAIWASKIPNIGIDITSNNKDLYSHAYFMNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIVESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFKELVKSIKELFLQKIQAKFEMAKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK" }, "paralog": false, "identity": 0.9890829694323144, "score": 805.0, "evalue": 1.6e-296, "description": "Internal stop codon at 181853, 181865, 181955. Nonsense mutation around 181853, 181865, 181955.", "psc": { "uniref90_id": "UniRef90_C4XEE1", "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE1", "kegg_orthology_id": "K03601", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE1", "EC:3.1.11.6", "UniRef:UniRef50_C4XEE1", "KEGG:K03601", "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE1" ], "product": "Exodeoxyribonuclease VII large subunit" } }, "id": "IGIGPGHMMC_376", "locus": "GCA000148625_00342" }, { "type": "cds", "sequence": "contig_1", "start": 183140, "stop": 183328, "strand": "+", "frame": 2, "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "RefSeq:WP_013354436.1", "SO:0001217", "UniParc:UPI0001E32FD5", "UniRef:UniRef100_C4XEE3", "UniRef:UniRef50_C4XEE3", "UniRef:UniRef90_C4XEE3" ], "nt": "ATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAA", "aa": "MSEKLKFNQIMNQIDEISNKLGSNIDLEDSINLYKQAEKLIEEAETKLVEVRKIVAKESIKQ", "aa_hexdigest": "bb796ea96a719634f36991ffb4c5135f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32FD5", "ncbi_nrp_id": "WP_013354436.1", "uniref100_id": "UniRef100_C4XEE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FD5", "RefSeq:WP_013354436.1", "UniRef:UniRef100_C4XEE3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEE3", "uniref90_id": "UniRef90_C4XEE3" }, "psc": { "uniref90_id": "UniRef90_C4XEE3", "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE3", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE3" ] }, "genes": [ "xseB" ], "id": "IGIGPGHMMC_377", "locus": "GCA000148625_00343" }, { "type": "cds", "sequence": "contig_1", "start": 183503, "stop": 183802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGTGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGA", "aa": "MKQIFANDTNSIKRIDVQKMYASKHKKLVNNLLDIKSKGDNATFFKTSNLSLDLKKLIGIKNLREILNNVEFKIALKNSFDSKKLNELKNINTVSQFEV", "aa_hexdigest": "2eef6b667d819f30d1c02bf12a9934cd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.177699999987, "isoelectric_point": 9.901650810241698 }, "id": "IGIGPGHMMC_378", "locus": "GCA000148625_00344" }, { "type": "cds", "sequence": "contig_1", "start": 184199, "stop": 185149, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTACTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTGTTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGA", "aa": "MTEALNSIKNIDSKYLNNLDYKETIFQEPFTEYSKVQLENNGCVELFPGTVLIEANPGGTKLREAMINLILNNKIDNLIEIDDLKDYTKTAYEKALGKGNIARVTPSDFSQEKAIIGTLNNSAIIIGPPGTGKSQTIANILVNIAKENKKALFISQKKVALDVVLKRLGKYQNMMFQFSETAKVNQSEKEYFYKPIIEYFKEINEVQENHDISKYNSSFFSKGEFKYLDAKSEIGEIFSKDLNCYFNLKNKGKIEQNIPKFIQFYKLYNELFSKNKFLKIFNNYKTLSKREIAYKTGYLKKYATIFTKLPAEFKRL", "aa_hexdigest": "8ca95db95325e990581f19c629de16e8", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 74, "aa_cov": 0.20253164556962025, "hmm_cov": 1.0, "evalue": 4.5308958845077945e-10, "score": 35.70896530151367, "start": 109, "stop": 172 } ], "seq_stats": { "molecular_weight": 36452.54659999996, "isoelectric_point": 9.223701286315915 }, "id": "IGIGPGHMMC_379", "locus": "GCA000148625_00345" }, { "type": "cds", "sequence": "contig_1", "start": 185222, "stop": 185689, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "ATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCAGCCAAGTAACTGA", "aa": "MSNFIRLNSFIEFDKNEKDYNKFLETKKSDNTTPELLEMIYENNAFEAKKRLDDYRQKSEAQRQKLNKFFGRLERKITPPYIISSLFKDIIKNVYNIYVGTPEILGNFIDFENDHYDYVIFDEASQIYIEKAIPFISIADKVIVAGDNQQMQPSN", "aa_hexdigest": "945dd55853591f3ee62037c74490ae5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 139, "aa_cov": 0.8903225806451613, "hmm_cov": 0.8920863309352518, "evalue": 5.372401436193887e-10, "score": 35.46651840209961, "start": 16, "stop": 153 } ], "seq_stats": { "molecular_weight": 18345.52439999998, "isoelectric_point": 5.138608741760252 }, "id": "IGIGPGHMMC_380", "locus": "GCA000148625_00346" }, { "type": "cds", "sequence": "contig_1", "start": 185771, "stop": 185929, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGAGGGTCAATGA", "aa": "MPKYLLEMNYRSNASELVLFSSKEFYESGLKGLDNYPRKEKTSVEVIDVEGQ", "aa_hexdigest": "1747e62b2a8fd188a931bb4f8e573a63", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6033.747500000001, "isoelectric_point": 4.9316011428832995 }, "id": "IGIGPGHMMC_381", "locus": "GCA000148625_00347" }, { "type": "cds", "sequence": "contig_1", "start": 185963, "stop": 186334, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGA", "aa": "MIQVCEKNIKKYNSIILLAFNRKQQDYIREQIALNSPKIFAELEDKVILRNIENIQGDEAELVIASVGYTGKTALHSTYIGSKKGRNALNVAITRAKDKMIIIKSIKTSEVDAKSENLMTFKR", "aa_hexdigest": "224945089dfb186540a8e58bbdf3938f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.8969072164948454, "evalue": 2.323008305264526e-12, "score": 42.88240432739258, "start": 7, "stop": 103 } ], "seq_stats": { "molecular_weight": 13944.106899999977, "isoelectric_point": 9.575053977966306 }, "id": "IGIGPGHMMC_382", "locus": "GCA000148625_00348" }, { "type": "cds", "sequence": "contig_1", "start": 186524, "stop": 186730, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAA", "aa": "MDNKSQFVVGLNVDSSSDDIEIDDFIEKRIKTDFMSVKGYPIYRVSNFRFAENQPSIYRFLNTLISKA", "aa_hexdigest": "6cac69579f4fd64bfaa62e3c79a728c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7914.845199999997, "isoelectric_point": 5.161571693420409 }, "id": "IGIGPGHMMC_383", "locus": "GCA000148625_00349" }, { "type": "cds", "sequence": "contig_1", "start": 187137, "stop": 187505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGTTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGA", "aa": "MLVAATAITLGSLYGCAKNSDEKLGKAHTGNPKDFKNYFVSVDKAESNFVTTDKSKKVAKFDFAKNQVIKENGQAISVDEFLDQYYREHHNLPFLNIKYGSFNFYNEYIEAVSPLEFQKFTN", "aa_hexdigest": "d0c87da07d27ae6434335fce4d9022c7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13893.434399999991, "isoelectric_point": 6.824737358093262 }, "id": "IGIGPGHMMC_384", "locus": "GCA000148625_00350" }, { "type": "cds", "sequence": "contig_1", "start": 187578, "stop": 187886, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGA", "aa": "MHGNSITLGAHSSKNKELTTIKFYPDAFFGTLPIYSELSGAGNAQDSLVYKVNQKLLTSEQLKDFLANISNYNAISNYSKTTLDNYGFRNLLDYRTLINQKV", "aa_hexdigest": "d236ab7c6e2c75a156e90a3fe3e31119", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11459.742699999986, "isoelectric_point": 8.860808753967284 }, "id": "IGIGPGHMMC_385", "locus": "GCA000148625_00351" }, { "type": "cds", "sequence": "contig_1", "start": 188160, "stop": 188258, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGA", "aa": "MDSYLNITFEDGTSFDVFNNFEDITHKNPKNE", "aa_hexdigest": "cb32795d8e0fccf327f9b3ecb853fab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3783.0048000000006, "isoelectric_point": 4.279487419128418 }, "id": "IGIGPGHMMC_386", "locus": "GCA000148625_00352" }, { "type": "cds", "sequence": "contig_1", "start": 189516, "stop": 189941, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAACAATGA", "aa": "MISKRIKGLFTPFITKNETLPENYLILGNHGDYPLTKFNIIEKFDQYDSKNILEYIDIVYNGLSTATNPHHKEGYKKYYTRIEEKIKAGDATAFNLYSQLKALQVKRFEIIEKQNDPKEDKTLLQAQLLSITYQFDQLKKQ", "aa_hexdigest": "e3e5c48b1305e074f841d7fa8220dd06", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16570.770799999984, "isoelectric_point": 8.889432716369633 }, "id": "IGIGPGHMMC_387", "locus": "GCA000148625_00353" }, { "type": "cds", "sequence": "contig_1", "start": 190002, "stop": 190496, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGCTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGA", "aa": "MLCKLDLNLQTAKKIAELHQSLINEYKQKRLELETIERKIELENFKILYMTQTANFADPVVIEQYNRILHKFEENINTHMPTYIAKVVDEQFKAADLKGKQVLEILKNPTTYNQVSIFISEIEKTNRYFKDFKNKVLLSQAELVAKIQVEANSDENLYNLNKKF", "aa_hexdigest": "ac3c2c0667ba131edcf6be81b7eeccb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19343.1264, "isoelectric_point": 7.757180976867676 }, "id": "IGIGPGHMMC_388", "locus": "GCA000148625_00354" }, { "type": "cds", "sequence": "contig_1", "start": 190560, "stop": 190982, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGA", "aa": "MTYEIEDNNNKKISVTKKAKERMFDSYAQEEDELNQVATHLTNRISTHQTVLDNINAKINQLQVEYKDPKITAERKVEIIELLKQIAKLYDNTKTAIYHINDVLREIKLAISNLLEIKQGITNGTHTYLDAYDLDSNLLS", "aa_hexdigest": "80eaa76e9341eaea77431378e92d201e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16194.129399999987, "isoelectric_point": 5.568140983581542 }, "id": "IGIGPGHMMC_389", "locus": "GCA000148625_00355" }, { "type": "cds", "sequence": "contig_1", "start": 191220, "stop": 191762, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTCTAGCTAAAAATTCAGAAATTGGTAATATGATTCTTGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGA", "aa": "MDVANKIETSLYKEAINKEIESTLAYKASENSIKNQIEFFSNLMPDYSKIINGLAKNSEIGNMILGLNIEVEKSCANDPLIKKLNNVLKDLDDPNSEINKFKEKAQNAYKTAGLKDQLDNIESEKSKYGKMTDSFIKLLNDTIKVKILSNYIKNESIDIESYVKNTNNEARLADTKDAVT", "aa_hexdigest": "5e3c6fe1d93397057903f4bfbcf77360", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20293.77760000001, "isoelectric_point": 5.243760871887206 }, "id": "IGIGPGHMMC_390", "locus": "GCA000148625_00356" }, { "type": "cds", "sequence": "contig_1", "start": 192018, "stop": 192248, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGA", "aa": "MEIIDKSRIEAIEKSMPKLNQDEKLVKYEAIINEGFQKTIDKLYNIDENLKTINLFDNAFERMAISKDFIETRTYD", "aa_hexdigest": "795d40a9bd9227430a9d7dbb8564dfd2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9034.215899999996, "isoelectric_point": 4.827301597595214 }, "id": "IGIGPGHMMC_391", "locus": "GCA000148625_00357" }, { "type": "cds", "sequence": "contig_1", "start": 192678, "stop": 193403, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGA", "aa": "MFNKAVDDHMKQTEKILKYQAPISNFFNEFNREYLTLLNPNIVAFEKIANSKIASIKTAIGELINARIREILISGREFPLYDSKNNGFEYKDADSIIVAKSRSELIDILSKKKLVNANDSVEKINKDIYFVRLSHLKKDGTKLVVTLRNILTDEKEMFANGYSDKYIKFNIDASVNDKRLSTLNALFTAANYKKQVTPTLIREEGNIRDENGKSVKGYSVFVDAYQDMTEKLLHEVPYAGE", "aa_hexdigest": "333bc44d9304527628d94babca1320b2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27578.191400000025, "isoelectric_point": 8.748891639709473 }, "id": "IGIGPGHMMC_392", "locus": "GCA000148625_00358" }, { "type": "cds", "sequence": "contig_1", "start": 193527, "stop": 193892, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGA", "aa": "MTDPNFKGISTDFLKFVGAHEYGHHITLNGAHDLGNKGNNPIYVSALTPGATPHIDNYYSKDALELYLKARTHLELNTTRLLKENNVVIDYGEYITFGIPKIEIVNGKPNLIYKNENEENI", "aa_hexdigest": "e3d3f63e6fd1a4e7ad53e1f909ebee3d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13631.155199999985, "isoelectric_point": 5.810559272766113 }, "id": "IGIGPGHMMC_393", "locus": "GCA000148625_00359" }, { "type": "cds", "sequence": "contig_1", "start": 193953, "stop": 194132, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGGCGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGA", "aa": "MQNFKGLLEAVIARRQANGLDGDYEKYLTAFDLWLMNSLDHHSGTINPSLRGGTAKYMV", "aa_hexdigest": "46ebd71bf313206d3e8c8b263a05b240", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6629.4735, "isoelectric_point": 6.703386116027832 }, "id": "IGIGPGHMMC_394", "locus": "GCA000148625_00360" }, { "type": "cds", "sequence": "contig_1", "start": 194313, "stop": 194576, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGA", "aa": "MFNEDGTPVINVPLHVDFNDKSSPYYDANAIKYVNSKIKAIIETIKSLIVEKYSINGWDTTATDLSLEPKFILSNLYLSTLLGKNDD", "aa_hexdigest": "40c2b22ba963ea8f116b9e1fcfc22e45", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9784.96219999999, "isoelectric_point": 4.748409080505371 }, "id": "IGIGPGHMMC_395", "locus": "GCA000148625_00361" }, { "type": "cds", "sequence": "contig_1", "start": 194643, "stop": 194987, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGA", "aa": "MIDDSKPLPYFDNSGKLIYPSNKKIPQASIYANIKKYALGIKRPEDLTSLMFAMNTAGDSFNNVVNSGEGHILYVDKDHQYLPNINLAQTFENNFLYNYVADMADVLQLKKIMK", "aa_hexdigest": "fc7fa4955eac2188585ff404244219ba", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12968.71679999999, "isoelectric_point": 6.711570930480956 }, "id": "IGIGPGHMMC_396", "locus": "GCA000148625_00362" }, { "type": "cds", "sequence": "contig_1", "start": 195114, "stop": 195305, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGA", "aa": "MSINEKIIEKDPVLSLFNAFYSIEGSQKLFGKDVAFTDYKKFLEFVSVDLKQAKLDKNTKVVN", "aa_hexdigest": "57091bbb1cd30df09ed7768297cc6004", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.293100000003, "isoelectric_point": 7.942972373962403 }, "id": "IGIGPGHMMC_397", "locus": "GCA000148625_00363" }, { "type": "cds", "sequence": "contig_1", "start": 195768, "stop": 196070, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGA", "aa": "MLESKNSVYGLLLTLGKGIFKKAKPSQDVLDYYGSKTERKFNEFFTDYTYNFAEVINRDNLQITYSPSNSNFGNMPSYLSNLSESNTGLEYVVDGAATSR", "aa_hexdigest": "95284cf6269465cfaa8a247f74256388", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.379299999991, "isoelectric_point": 5.302987098693847 }, "id": "IGIGPGHMMC_398", "locus": "GCA000148625_00364" }, { "type": "cds", "sequence": "contig_1", "start": 196146, "stop": 196292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGA", "aa": "MLNEEARRRSQILNTRFIERVFANQDNMQYGQNYKNNYFGQFKTINNG", "aa_hexdigest": "1dfe9e9dded48c15f5990b26ac6cfcd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.407800000001, "isoelectric_point": 9.812748908996582 }, "id": "IGIGPGHMMC_399", "locus": "GCA000148625_00365" }, { "type": "cds", "sequence": "contig_1", "start": 196509, "stop": 196763, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGA", "aa": "MFGYLSTAIADKANYLAFKNVRTGEVKTLRITKQNASNMFYYKEQQVNNEIRHQNGEKVRHYLIDEPFDFTDKNGHQKGTGFVA", "aa_hexdigest": "87fccb8041248ebca426ed11b6fc2cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9747.825799999997, "isoelectric_point": 9.259610176086426 }, "id": "IGIGPGHMMC_400", "locus": "GCA000148625_00366" }, { "type": "cds", "sequence": "contig_1", "start": 196782, "stop": 196889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGA", "aa": "MSRYRNRLLDTNEEYYVYFASDEQGNKALEADLGT", "aa_hexdigest": "45c3ce5d1dbd9e45a270f0d42f4d2ea9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4133.422600000001, "isoelectric_point": 4.440682792663575 }, "id": "IGIGPGHMMC_401", "locus": "GCA000148625_00367" }, { "type": "cds", "sequence": "contig_1", "start": 197036, "stop": 197521, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGTGAAAGTAGATGA", "aa": "MKRNKLILSLASLSTISTASIVAVSCGNKAYKTNYDLGLVTEPINSLNYLKFNSVSKVLPSIVESPLKTGPNEALKRILSLPEIPMGVYGNDDKSNSMDDYIANNRAPKEASGRFYPLDQFGSAPGTINTDRSEYQPVSVVMTKNNKILSLHITLNRGESR", "aa_hexdigest": "00fcbe3ecaa3d9c41544572d195dd2dd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17610.908699999993, "isoelectric_point": 9.324529838562015 }, "id": "IGIGPGHMMC_402", "locus": "GCA000148625_00368" }, { "type": "cds", "sequence": "contig_1", "start": 197564, "stop": 197740, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGA", "aa": "MHYILDINTGSQKQTNILQRKFRSSSSLIDAQQEYIKKHKKAYSNPFAYPKLIKENGQ", "aa_hexdigest": "c4826ade9e167ede28aa5312607bf347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6799.682800000002, "isoelectric_point": 9.85201015472412 }, "id": "IGIGPGHMMC_403", "locus": "GCA000148625_00369" }, { "type": "cds", "sequence": "contig_1", "start": 197951, "stop": 198634, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTACTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGA", "aa": "MLPNPEYSLSRHTAEELKLIPQRIATKIRKYLYFDPRQSYSETKFKPLVKKAKKLKSRMNKNVSFEKDINEYNQEVNKLYGKENTLNNNSIDSREFMENRVLALDEFSLRVEYDSNEPTSLSNAYSDIQSTLIPVNRKFVESIGGIREFGLDKSKFLTNGPFYIKNIVLGPQGYMELVKNNTYYSSTKTITDSIKIYFSSDANINSAMYDDGYIAATKIPAVQQINY", "aa_hexdigest": "347a906022a771669176a6cf46ed732e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26232.408200000016, "isoelectric_point": 9.070331001281737 }, "id": "IGIGPGHMMC_404", "locus": "GCA000148625_00370" }, { "type": "cds", "sequence": "contig_1", "start": 198659, "stop": 198808, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGA", "aa": "MKKSSGFGTIALAFNLDQETNKNSLINDVDLRNALYFGINRNEMLNIVG", "aa_hexdigest": "3d45a261e11820698e48f301f580578c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5461.146999999999, "isoelectric_point": 6.0008562088012685 }, "id": "IGIGPGHMMC_405", "locus": "GCA000148625_00371" }, { "type": "cds", "sequence": "contig_1", "start": 198902, "stop": 199369, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGA", "aa": "MKTKVSDKKIPIQNYNHVDHLAKQYNNEHVDRTDLGYNLEIARAYMQRFKDKHPDVKQVTLKYISNSTDEQQNAGIALKDFIKKAFGDYLIIDVQGLPENVYEDFRTTGKYDLIYRNFDTFGSDSYSYVKVFFKPDEIDRKNQKTTGFRNNPSGS", "aa_hexdigest": "10a1e4ba01d5dafbc5d51422879e05b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18135.033199999983, "isoelectric_point": 8.583530235290528 }, "id": "IGIGPGHMMC_406", "locus": "GCA000148625_00372" }, { "type": "cds", "sequence": "contig_1", "start": 199856, "stop": 199969, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGA", "aa": "MQKRNDQNYFTGTVNKDQYNLYNREVDFSGKAKKDNR", "aa_hexdigest": "4d5062402735ed610fcae5a083d11ceb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.849100000002, "isoelectric_point": 9.520900535583493 }, "id": "IGIGPGHMMC_407", "locus": "GCA000148625_00373" }, { "type": "cds", "sequence": "contig_1", "start": 200303, "stop": 200662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCAAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGA", "aa": "MIGIFSVFLTLIIGIPVGVYVGMNPDKLPDHIATLIVSVFSSIPSLVFALLLLLIGRSLNMPYLFNELDIATYVLPGLALSLGSIIVYIKYIRTELNQELNSQHAKFCYLKGLSKRRFV", "aa_hexdigest": "f0adee19b5fe0287a406edbaa0f3eb3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13244.76619999998, "isoelectric_point": 9.166517829895021 }, "id": "IGIGPGHMMC_408", "locus": "GCA000148625_00374" }, { "type": "cds", "sequence": "contig_1", "start": 200741, "stop": 200923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAG", "aa": "MFVEQIFFITGSGGLLIQAITSKDYNIILFMVTLFSLITILSYACRDALYQIIDPRVRRR", "aa_hexdigest": "a44c8e6b125770220820a8f7800758e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6946.2058, "isoelectric_point": 9.098052406311037 }, "id": "IGIGPGHMMC_409", "locus": "GCA000148625_00375" }, { "type": "cds", "sequence": "contig_1", "start": 201098, "stop": 201319, "strand": "+", "frame": 2, "gene": null, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "nt": "ATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGA", "aa": "MHKQKKPFLEFFYRFSRSTAGVFGFVALALLIILAIFLPFTTKDPNTVNTEKRYLIFFTDGHIFGTDALGRDI", "aa_hexdigest": "76472b4940abb58951f87b0170df7f8a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAE2EIN1", "query_cov": 1.0, "subject_cov": 0.9733333333333334, "identity": 0.6579999999999999, "score": 102.0, "evalue": 2.4e-27, "valid": false, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "uniref50_id": "UniRef50_A0AAE2EIN1" }, "pscc": { "uniref50_id": "UniRef50_A0AAE2EIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "product": "N-terminal TM domain of oligopeptide transport permease C family protein" }, "genes": [], "id": "IGIGPGHMMC_410", "locus": "GCA000148625_00376" }, { "type": "cds", "sequence": "contig_1", "start": 201710, "stop": 201934, "strand": "+", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "nt": "GTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAA", "aa": "MFHIPGVILSETSLAFIGLSITDIPTLGNLINDGSKIFTTFPRYVLIPSTMLIIVTTSIQLMSAAIQDSLLRQR", "aa_hexdigest": "ff062bc2a790fe596087ebfb724b0aab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGD7", "query_cov": 1.0, "subject_cov": 0.8809523809523809, "identity": 0.662, "score": 100.0, "evalue": 2.64e-26, "valid": false, "uniref50_id": "UniRef50_A0A3B0PSU9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PSU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "product": "ABC transmembrane type-1 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_411", "locus": "GCA000148625_00377" }, { "type": "cds", "sequence": "contig_1", "start": 202227, "stop": 202994, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3", "UniRef:UniRef90_A0A3P8MDR3" ], "nt": "GTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAGCCTAAAATTTTAGTCATGGATGAGCCTACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCGATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGA", "aa": "MAAEIEDVYLTFRNPADPKEKNLVLRGPSLKIYEGKVHAIIGESGSGKSVITSLLYGLTGSNAIIESGKIKLYGLEVQDYTMYNWEKSKLRGRVVSAVFQNPMSILDPTMKVGKQIIEGMLINKIVKTKKEAKAEAIKYLEMTKINNPQKVMDLYPHELSGGMIQRIAIAAIVSLKPKILVMDEPTTALDPTVQALVLDIIRELQERFHIAIAFITHDLGVVASISDFINIMYAGQIIESGTKEEILQNPQHPYT", "aa_hexdigest": "fccfa6d2da0b76016ecd842b85b4407c", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR3", "query_cov": 0.8117647058823529, "subject_cov": 1.0, "identity": 0.9520000000000001, "score": 376.0, "evalue": 1.63e-130, "valid": true, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MDR3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_412", "locus": "GCA000148625_00378" }, { "type": "cds", "sequence": "contig_1", "start": 203010, "stop": 203243, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07", "UniRef:UniRef90_A0A3P8LB07" ], "nt": "ATGCCTGACTTTAACAAAGGCACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCGGTTAGAAATGACTATGCTTTAGGAATAGATTTTGAAGAAGAATCGCCTTTCTATCAAATTTCTCCTACACACTTTGTTAAAAGTAATTTATTTAATGAACAAGCTCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGA", "aa": "MPDFNKGTKLQVIRGAVPSNLNNIDGDAFAVRNDYALGIDFEEESPFYQISPTHFVKSNLFNEQAPKYEPPKIIQNL", "aa_hexdigest": "a8630675bfe4f39b70141d75366b63ff", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 203010, "stop": 203435, "observations": { "mutation": [ 203241 ], "stop": [ 203241 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LB07", "query_cov": 1.0, "subject_cov": 0.5460992907801419, "identity": 0.8440000000000001, "score": 135.0, "evalue": 1.55e-39, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MPDFNKDSKLQVIRGAVPSNLNNIKGDAFAVRNDYALGIDFEEESPYYQISTTHFVKSNLLNKNVSKYEPPKIIKRLWDKYENQMNSIYGDDKYVSQKVYDEFVQKAYENNELVKKLIAKREEEKAIKQSTEVKKPGEKNA" }, "paralog": false, "identity": 0.7163120567375887, "score": 203.0, "evalue": 1.05e-68, "description": "Internal stop codon at 203241. Nonsense mutation around 203241.", "psc": { "uniref90_id": "UniRef90_A0A3P8LB07", "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LB07", "UniRef:UniRef50_A0A3P8LB07" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8713.666199999994, "isoelectric_point": 4.870215034484862 }, "id": "IGIGPGHMMC_413", "locus": "GCA000148625_00379" }, { "type": "cds", "sequence": "contig_1", "start": 203428, "stop": 203769, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGCACAGAGGAAATGCTTAATGTATATCCAGATAAAAAACCTTTAGTAGAAATAAGACATTTAGATATTGATTATGGTTTTGGAACTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGTGAAGTTTTAGGACTTGTTGGTGAAAGTGGTAGTGGTAAAAGTACTACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGA", "aa": "MPKYYYEKRKFGKPLAREIRQGTEEMLNVYPDKKPLVEIRHLDIDYGFGTKKFSAIKDLNLNIYDGEVLGLVGESGSGKSTTGRAIIGLTPHTYGYIKILDRIIPKNLDKGFK", "aa_hexdigest": "85f922bdf7999cc2f69d5e64ada44b48", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 46, "aa_cov": 0.40707964601769914, "hmm_cov": 1.0, "evalue": 4.3537582468940035e-12, "score": 42.82134246826172, "start": 57, "stop": 102 } ], "seq_stats": { "molecular_weight": 12852.758099999985, "isoelectric_point": 9.467004966735843 }, "id": "IGIGPGHMMC_414", "locus": "GCA000148625_00380" }, { "type": "cds", "sequence": "contig_1", "start": 203800, "stop": 204666, "strand": "+", "frame": 1, "gene": null, "product": "Oligopeptide ABC transporter, ATP-binding component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "nt": "ATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAACTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACAGGCTTTGATCAAACAGTTTTAAAAGAAGTAAATAGATTGGTAAATAAAATTGATCCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATGACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAAATAATTTAAGTGAAAAAGAATTTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTATACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATTGCCGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAGGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCTGATAGAATAGCTGTTATTAATAAAGGCGTCTTACTTGAAGTAGGTCCAACCGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCTGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGA", "aa": "MVNKVQMIFQDPTNSLNPYKNVETVVGEGLTNLKNSKYIFLTGFDQTVLKEVNRLVNKIDPTNSLISNLLEKCREIEVSIEKSYDFVFKELIDEINRRYTTNANYKDALEYLESQKVERDSLNNLSEKEFKKRLIIDILKQVGLDETILGRFPLEFSGGQQQRLGICRSVVLQPKLLIADEPISALDVSIQAQVINIFNELKEKYNLTILFIAHDLRMVEYISDRIAVINKGVLLEVGPTDEIIHNAHHPYTRSLLDAVPSIESEKGSLLGYIYDPSMHKYDENNQPV", "aa_hexdigest": "10b264329330af2e433048340621f488", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q6MRZ9", "query_cov": 1.0034722222222223, "subject_cov": 0.903125, "identity": 0.64, "score": 355.0, "evalue": 7.09e-120, "valid": false, "product": "Oligopeptide ABC transporter, ATP-binding component", "uniref50_id": "UniRef50_Q6MRZ9" }, "pscc": { "uniref50_id": "UniRef50_Q6MRZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "product": "Oligopeptide ABC transporter, ATP-binding component" }, "genes": [], "id": "IGIGPGHMMC_415", "locus": "GCA000148625_00381" }, { "type": "cds", "sequence": "contig_1", "start": 204691, "stop": 205524, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAGCTACCAATGAAGAATTAGCTGAATACAAAAGAGATAGAGCTAAACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTAAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGATGTTAAAGAACCTAAACCAAAGGTTGAAAAATCTAAAGTCAAAATGCCAAAAGACAGACCAAGTGTTCGTGTAACTCCTTCTTATATAGAAGTAGCTAAAGTTATTGAAGATGAAGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAATCATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAATTAGTAGAAAAACTTGCTAAAGAAAATGAATAA", "aa": "MLATNEELAEYKRDRAKLNEIEAKRERNEKITLDDFIIKENVINEQEKYDLVTKIKEAQIDLNNYNEGSEVKITPSYVKTATVIQEEVSTYDSLKETTKLIKEDDLPEVKVTPSYIEVAKVIDVKEPKPKVEKSKVKMPKDRPSVRVTPSYIEVAKVIEDEEIKKTEKSSTKKVEKITIISNVHKDSTVELIPENDKITVEEKPKKVRKTRKVSATLKKPTKKSTTKKQTKSSTITVDLSKKIKPNKGVTKAELRAQARKEKSANKKLVEKLAKENE", "aa_hexdigest": "8c17eb84b775cec9647a6399f58ccab0", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31707.203100000053, "isoelectric_point": 9.431740760803223 }, "id": "IGIGPGHMMC_416", "locus": "GCA000148625_00382" }, { "type": "cds", "sequence": "contig_1", "start": 205562, "stop": 205966, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354444.1", "SO:0001217", "UniParc:UPI0001A8C120", "UniRef:UniRef100_C4XEF3", "UniRef:UniRef50_C4XEF3", "UniRef:UniRef90_C4XEF3" ], "nt": "ATGGATAAGGCTAAGAAAAAGAAAATTTTAATCCCACTCTTAAGTACTGTTGGTGTTTTAGCTGTTGTTACTCCAATTGTTGTTGTATCTGCAGCTAAAAGTAAACATAAGGAAATTGAAAAACAAGAAACAATAAATTCTTTTGAAGAATTTCCTAAATTAGAATATAACAACTATTACAAGTACATTAGAATAGGTGAAAAAGGCGAAGAATATATTGATGAAAATGTTGTGCCTTTAATAGTGAAAGATGTTTTTCAAAAACTAAACGCAGATTATCAAAGTAAAATTTCTTTTGATTATCAATTTGTAAGCAAAAATCATTTAGAACTTAAATTTAAATATGTTTCAGAAGACAAAACACTTTTCAAAACTTATGTTTTAAAAACTAAAAATATGGTTTAA", "aa": "MDKAKKKKILIPLLSTVGVLAVVTPIVVVSAAKSKHKEIEKQETINSFEEFPKLEYNNYYKYIRIGEKGEEYIDENVVPLIVKDVFQKLNADYQSKISFDYQFVSKNHLELKFKYVSEDKTLFKTYVLKTKNMV", "aa_hexdigest": "b103e33d00b36b030ab5faad621b356a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C120", "ncbi_nrp_id": "WP_013354444.1", "uniref100_id": "UniRef100_C4XEF3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C120", "RefSeq:WP_013354444.1", "UniRef:UniRef100_C4XEF3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEF3", "uniref90_id": "UniRef90_C4XEF3" }, "psc": { "uniref90_id": "UniRef90_C4XEF3", "uniref50_id": "UniRef50_C4XEF3" }, "pscc": { "uniref50_id": "UniRef50_C4XEF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15662.085599999993, "isoelectric_point": 9.171546363830569 }, "id": "IGIGPGHMMC_417", "locus": "GCA000148625_00383" }, { "type": "cds", "sequence": "contig_1", "start": 206829, "stop": 207299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTGCATGATATTTCGCAAGAACGTTATGTATACAAATCAGAATATTTAGATGCTCCATCTTATTTAACTAAAGATCACTATGATGTAAATTTTAAAGATGCATGCAATATTGTAAATGGTAATATTGAAATAAAAAGATATTTTACAGAAGATAGATTTGCAATTAAAATATTTTTTATTCAACCCAAAAATTTACATAAAAAATTCTTTGAAGGTTTTGATATTGAAATAAATGGAAAAAGAATTGATGGAAACATAATTAGAAGCTGCACAAAATTGGAAACAATGGATAGCAGTTTACTAAATGAAAGACTTAGTGTTAAAAAATGGTATTCGAAATTTCCTGAAGAAACAAGTGCATATGGATTAACAATCCTTTCACCTAAAATTATAAATTTACCTTACGAAAAATTCCGTATTTTAAGAGTTTACTTTAGAAGTAAAGAAAAAACAGAAAAACAATATTGA", "aa": "MVHDISQERYVYKSEYLDAPSYLTKDHYDVNFKDACNIVNGNIEIKRYFTEDRFAIKIFFIQPKNLHKKFFEGFDIEINGKRIDGNIIRSCTKLETMDSSLLNERLSVKKWYSKFPEETSAYGLTILSPKIINLPYEKFRILRVYFRSKEKTEKQY", "aa_hexdigest": "27390ae8b7cd008f0b3fb964e94cf1f7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18707.2531, "isoelectric_point": 9.052537727355958 }, "id": "IGIGPGHMMC_418", "locus": "GCA000148625_00384" }, { "type": "cds", "sequence": "contig_1", "start": 207409, "stop": 207666, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5", "UniRef:UniRef90_C4XEF5" ], "nt": "ATGAATCTAACTTTTAATGATTATTTTATGGGCTTAATTTCACATAAAGATCAAAATAGTGTTTTACACAATATCTTTAAAATGGAAAAAGTCAATGAGCAAGCTTACAAAAAGACAATTGAAGGAGGCAACAAATCAAACATACTTAAAAACATCTTTAAACCAAAAAATAAAAGTCAGCATATTTTATCTATTATGAAACCTGAACTTGCGCAAATTATCAAAGAGGATTTCTTAAAAAGTCAATCTAAAAATTGA", "aa": "MNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIEGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKN", "aa_hexdigest": "da5eca6da596cc1400111a33b0c0e939", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 207322, "stop": 207681, "observations": { "mutation": [ 207411 ], "stop": [ 207411 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF5", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 0.988, "score": 163.0, "evalue": 1.18e-50, "gene_start": 1, "gene_end": 85, "reference_start": 6, "reference_end": 90, "reference_sequence": "MERRKMNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIGGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKNWFKDYYSKNTYYKYKKQAVEEFLYHYFNE" }, "paralog": false, "identity": 0.9747899159663865, "score": 237.0, "evalue": 3.05e-82, "description": "Internal stop codon at 207411. Nonsense mutation around 207411.", "psc": { "uniref90_id": "UniRef90_C4XEF5", "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF5", "UniRef:UniRef50_C4XEF5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9911.421599999996, "isoelectric_point": 9.657960319519042 }, "id": "IGIGPGHMMC_419", "locus": "GCA000148625_00385" }, { "type": "cds", "sequence": "contig_1", "start": 207849, "stop": 208049, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAGAGTTGATAAAGATTCCTTATTAAAACAAGCTGAATTTAGTCATGTTTCTCAAGATGAATTTAAAGACTATCATAAAAATATTATTAATAATACTTTCCCTTTAACTATTGGCGGTGGTATAGGGCAAAGTAGACTTTCAATGTTCTTGTTAGAGAAGAAACACATTGGTGAAGTTCAAGTAGGTATTTGA", "aa": "MGIRVDKDSLLKQAEFSHVSQDEFKDYHKNIINNTFPLTIGGGIGQSRLSMFLLEKKHIGEVQVGI", "aa_hexdigest": "28e07f933ce3c23cef55071f2989b38e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.448299999997, "isoelectric_point": 6.820531272888183 }, "id": "IGIGPGHMMC_420", "locus": "GCA000148625_00386" }, { "type": "cds", "sequence": "contig_1", "start": 208113, "stop": 208382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAAAAAAATTGTCAATACAATTTTTAAATGTGTTCGTCATGTTGAATACAAACTTTTAGTTGAATTTCCACAACTAAGTCAAAAATTACCTGAAAATGTAACTTTTATTAGTAGTAGTGAACTTTACAACTTATATCCAAAATTAAGCCCTGAAGAAAGAGAAACAAAATTTGCTAAAAAACACAAGGCTATATTTATTTATGAAATTGGTTATCCTTTAGCAGATAATAAGCCTCAATCAAAACGTGCTTTTGACTACGATGACTGA", "aa": "MKKIVNTIFKCVRHVEYKLLVEFPQLSQKLPENVTFISSSELYNLYPKLSPEERETKFAKKHKAIFIYEIGYPLADNKPQSKRAFDYDD", "aa_hexdigest": "2a94330fbbd337e73f2d7c2d41acbb51", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 89, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.4909297263456703e-29, "score": 98.67347717285156, "start": 1, "stop": 89 } ], "seq_stats": { "molecular_weight": 10559.090099999987, "isoelectric_point": 8.967632865905763 }, "id": "IGIGPGHMMC_421", "locus": "GCA000148625_00387" }, { "type": "cds", "sequence": "contig_1", "start": 208548, "stop": 208778, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGTACAAGAGTAAATTAAACATTAAAGAAACTCAATTAGCAATTCAAGATTTGAAGTTTGCATTTACTAAAAAATTAAACAAGGAATTACATTTAACTAGAGTTTCAGCCCCTTTATTTGTTGCTAGAAATACCAAAATAAATGATGGCTTAAATGGTGAAGAACCTGTGTCTTTTGTTCCTAAAAATTTTGATGAAACTGTTGAAATAGTTCACTCTCTTGCTAAATGA", "aa": "MYKSKLNIKETQLAIQDLKFAFTKKLNKELHLTRVSAPLFVARNTKINDGLNGEEPVSFVPKNFDETVEIVHSLAK", "aa_hexdigest": "fd57b4d2e512df91a6ada4ac12db9f79", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 2.254619603068207e-19, "score": 66.10094451904297, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8657.9479, "isoelectric_point": 9.347029304504396 }, "id": "IGIGPGHMMC_422", "locus": "GCA000148625_00388" }, { "type": "cds", "sequence": "contig_1", "start": 208765, "stop": 208926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCATTGTTAATTTACATATCACAATTATCAATGATGTTACACATCTTGAAAACAACAAAAAAAGCTATGCAAGAAGCTAAAAATAATCAAGAAAATTCAATTCCGCAAAATGACCAAAACAACTATAAAACAGAGGCTAACGAAAATGTACAAGAGTAA", "aa": "MSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE", "aa_hexdigest": "d104dafb46ec731f98f5447d9804ae5b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6168.919100000002, "isoelectric_point": 5.697790718078613 }, "id": "IGIGPGHMMC_423", "locus": "GCA000148625_00389" }, { "type": "cds", "sequence": "contig_1", "start": 209005, "stop": 209364, "strand": "-", "frame": 2, "gene": null, "product": "PQ loop repeat", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7", "UniRef:UniRef90_C4XEF7" ], "nt": "ATGGCGGCTGTAGTTTATGCCAATATTTTGTGTGCTTATGTCTATTGTGTACTTTTATTCTTTATGTACAAATATGATGAAAAAGTAAAAAGAAACAAAAGAAAATTTGTAGTTTTAGGAATAACATTAACTTTAACTACAATTTGTGCAATTGTTGGTTTTGTCGGTTTATATCACAAAAACAGTGAAGGCAATGGCCTTAATTTTGATCCTAATACAACAGCTATTTTGTCTCAAATTATTCCAGTTATATCAACTTTTGCATTCTTACCACAAGTATTAAAAACATTTGAAAAGAGAGATATTGAAGGCTTATCAGTTGGTTTAATTTTAATGTTTGTATTAACAAATATTTTCTGA", "aa": "MAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIF", "aa_hexdigest": "c7f5c9bfba956729557ae0903bac8929", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 208765, "stop": 209604, "observations": { "mutation": [ 208935, 208995, 209007, 209403, 209430 ], "stop": [ 208935, 208995, 209007, 209403, 209430 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF7", "query_cov": 1.0, "subject_cov": 0.4265232974910394, "identity": 1.0, "score": 233.0, "evalue": 1.6e-75, "gene_start": 1, "gene_end": 119, "reference_start": 81, "reference_end": 199, "reference_sequence": "MDFIRSSFAINKDVNDGIAAFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSFWIFFAALCSWIIFGSFTGEEAKMAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIFWIAYWVSLIIAEGKLTPALTSTLLWQTLSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE" }, "paralog": false, "identity": 0.978494623655914, "score": 521.0, "evalue": 1.57e-191, "description": "Internal stop codon at 208935, 208995, 209007, 209403, 209430. Nonsense mutation around 208935, 208995, 209007, 209403, 209430.", "psc": { "uniref90_id": "UniRef90_C4XEF7", "product": "PQ loop repeat", "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF7", "UniRef:UniRef50_C4XEF7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7" ], "product": "PQ loop repeat" } }, "id": "IGIGPGHMMC_424", "locus": "GCA000148625_00390" }, { "type": "cds", "sequence": "contig_1", "start": 209428, "stop": 209604, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTATACGTAGTTCTTTTGCTATAAATAAAGATGTTAATGATGGCATAGCAGTGTTTGTAGCAATAGTAGGAATGATAGCATGTATTGCAACAATAAGTGTATCAATTCCTCAATTAGTTTATTTATTAAAAAGAAAGAAAACTGGGGAGGTTAAATTCTATTCATTCTGA", "aa": "MDFIRSSFAINKDVNDGIAVFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSF", "aa_hexdigest": "a89000615785da4ec2988b7de5414003", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6429.612599999999, "isoelectric_point": 9.35966510772705 }, "id": "IGIGPGHMMC_425", "locus": "GCA000148625_00391" }, { "type": "cds", "sequence": "contig_1", "start": 209810, "stop": 210235, "strand": "+", "frame": 2, "gene": null, "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354449.1", "SO:0001217", "UniParc:UPI0001E33001", "UniRef:UniRef100_UPI0001E33001", "UniRef:UniRef50_C4XEF8", "UniRef:UniRef90_C4XEF8" ], "nt": "ATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTCTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAACTTAAAGCTTTTTATTATTATATGGATTGTGAATGTAGTCCTTGCAAGCCAAACAAAGAATATGTTGAAATGTGTTTAAAAGGATACCAAGACTGCAATATTAAAAAAGAACAACTTAGAGAAATTTTATACAAGTAA", "aa": "MKKYYIAYGSNLNSGHMENLCPSAKFLGKVILNNFSLSFEGEKDRCYLNIRKVKDKNIEVGVFELNDKDIFKLDEYEDYPFLYKKEDIEIELNSKKLKAFYYYMDCECSPCKPNKEYVEMCLKGYQDCNIKKEQLREILYK", "aa_hexdigest": "bb1a48c9b6cc88534006ae7f156d4c1b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33001", "ncbi_nrp_id": "WP_013354449.1", "uniref100_id": "UniRef100_UPI0001E33001", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33001", "RefSeq:WP_013354449.1", "UniRef:UniRef100_UPI0001E33001" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33001", "uniref90_id": "UniRef90_C4XEF8" }, "psc": { "uniref90_id": "UniRef90_C4XEF8", "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "uniref50_id": "UniRef50_C4XEF8" }, "pscc": { "uniref50_id": "UniRef50_C4XEF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF8" ], "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_426", "locus": "GCA000148625_00392" }, { "type": "cds", "sequence": "contig_1", "start": 210273, "stop": 210737, "strand": "+", "frame": 3, "gene": null, "product": "Fic family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F02C33E", "UniRef:UniRef90_UPI000F02C33E" ], "nt": "ATGATCAACAAGGAATATAAACCTGTGTTCAAGGTTACAAACAAAATAATAGAATTGGTTGGCAAGATTAAAGAAAAATTGTCAATCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAATGCTTTAAAAGCCTATAAAACAATTGAAGAAGTTAAAAATAACAATGTAGAAAATTTTTTAAAAATACATAAAATTCTTATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGA", "aa": "MINKEYKPVFKVTNKIIELVGKIKEKLSIVFLCKDFLILNRKIKIKSIYSSVKIEGNPYDFQTFYKVINNEEKEQKDKYKLEFKNALKAYKTIEEVKNNNVENFLKIHKILMNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFE", "aa_hexdigest": "02f8816d370db03305567905d2097f9c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 210273, "stop": 211040, "observations": { "mutation": [ 210735 ], "stop": [ 210735 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F02C33E", "query_cov": 1.0, "subject_cov": 0.47384615384615386, "identity": 0.9940000000000001, "score": 293.0, "evalue": 8.54e-98, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MINKEYKPVFKVTNKIIELVGKIKEKLSIVFLCKDFLILNKKIKIKSIYSSVKIEGNPYDFQTFYKVINNEEKEQKDKYKLEFKNALKAYKTIEEVKNNNVENFLKIHKILMNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFEWYNNDISDVLIKSCAFHYELEFIHPFEDGNGRIGRLWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEAYDFDSLNEVEKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKIMIIGKLNSPKVKYKLVENNKE" }, "paralog": false, "identity": 0.984313725490196, "score": 511.0, "evalue": 6.11e-186, "description": "Internal stop codon at 210735. Nonsense mutation around 210735.", "psc": { "uniref90_id": "UniRef90_UPI000F02C33E", "product": "Fic family protein", "uniref50_id": "UniRef50_UPI000F02C33E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F02C33E", "UniRef:UniRef50_UPI000F02C33E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F02C33E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F02C33E" ], "product": "Fic family protein" } }, "id": "IGIGPGHMMC_427", "locus": "GCA000148625_00393" }, { "type": "cds", "sequence": "contig_1", "start": 210840, "stop": 211250, "strand": "+", "frame": 3, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9", "UniRef:UniRef90_C4XEH9" ], "nt": "TTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTCATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATAAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGTCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTGGAACAGCAAAAAATAATGATTATTGGTAAATTAAATTCACCTAAAGTAAAATATAAATTAGTAGAAAACAATAAAGAGTAA", "aa": "MWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNKIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKIMIIGKLNSPKVKYKLVENNKE", "aa_hexdigest": "ecc16ac9e2bbbbb20e9d57d36cac7453", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 210585, "stop": 211250, "observations": { "mutation": [ 210735 ], "stop": [ 210735 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH9", "query_cov": 1.0, "subject_cov": 0.6153846153846154, "identity": 0.971, "score": 264.0, "evalue": 3.34e-88, "gene_start": 1, "gene_end": 136, "reference_start": 86, "reference_end": 221, "reference_sequence": "MKIHKILMNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFEWYNNDISDVLIKSCAFHYELEFIHPFEDGNGRIGRLWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKVIIIGKLNSPKVKYKLVENNKE" }, "paralog": false, "identity": 0.9773755656108597, "score": 438.0, "evalue": 2.9e-159, "description": "Internal stop codon at 210735. Nonsense mutation around 210735.", "psc": { "uniref90_id": "UniRef90_C4XEH9", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH9", "UniRef:UniRef50_C4XEH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9" ], "product": "Fido domain-containing protein" } }, "id": "IGIGPGHMMC_428", "locus": "GCA000148625_00394" }, { "type": "cds", "sequence": "contig_1", "start": 212088, "stop": 212675, "strand": "+", "frame": 3, "gene": null, "product": "Abortive phage infection protein", "db_xrefs": [ "RefSeq:WP_013526752.1", "SO:0001217", "UniParc:UPI0001F5A11B", "UniRef:UniRef100_C4XEI1", "UniRef:UniRef50_C4XEI1", "UniRef:UniRef90_C4XEI1" ], "nt": "ATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAA", "aa": "MNYNEKLEKVIEECNGVVTTKECNKRNIPNSIISRAAKKGRIEKYSEGIYVAEEIPMDPHFLFQLRFPRSIFSYESALQLHGVSERFEKALEVTVNTGYKFNDTKKIFPHYVKDEYLELGVEEIKTSQGNKVRVYSLERTFCDMVANRNKVDAETFVKFMITFQGIKINYSQLNSIAKKMGVINKVVDVMGIIRE", "aa_hexdigest": "939390d8773cf80f17abb4e440145976", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F5A11B", "ncbi_nrp_id": "WP_013526752.1", "uniref100_id": "UniRef100_C4XEI1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A11B", "RefSeq:WP_013526752.1", "UniRef:UniRef100_C4XEI1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI1", "uniref90_id": "UniRef90_C4XEI1" }, "psc": { "uniref90_id": "UniRef90_C4XEI1", "product": "Abortive phage infection protein", "uniref50_id": "UniRef50_C4XEI1" }, "pscc": { "uniref50_id": "UniRef50_C4XEI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI1" ], "product": "Abortive phage infection protein" }, "genes": [], "id": "IGIGPGHMMC_429", "locus": "GCA000148625_00395" }, { "type": "cds", "sequence": "contig_1", "start": 212701, "stop": 213405, "strand": "+", "frame": 1, "gene": null, "product": "Abortive infection protein AbiGII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2", "UniRef:UniRef90_C4XEI2" ], "nt": "TTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGA", "aa": "MVSKKFDIDYNSIRKLFYLEEILKRIAKSKYRNNFIFKGGMLFSNIIGIENRRTVDIDFSLKGIESNEKNLKIVFNEILLIKNDEINFIFNDIQPIKTDLNYPCYRFKFRASLENEKEDIVLDIGVGDKIFPKPIEYSYKSFFKDESFQVLSYTLETYFSEKLFAIISKGEANTRLKDYYDLHFLILNCKKMIDKSNLGKAISITFDNRNEKFDIKTAKLKLLELSNFELLDNK", "aa_hexdigest": "9028a0862dc82d4cacd999c7fbaffc0f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEI2", "query_cov": 1.0, "subject_cov": 0.8447653429602888, "identity": 0.996, "score": 459.0, "evalue": 1.52e-162, "valid": true, "product": "Abortive infection protein AbiGII", "uniref50_id": "UniRef50_C4XEI2" }, "pscc": { "uniref50_id": "UniRef50_C4XEI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2" ], "product": "Abortive infection protein AbiGII" }, "genes": [], "id": "IGIGPGHMMC_430", "locus": "GCA000148625_00396" }, { "type": "cds", "sequence": "contig_1", "start": 213550, "stop": 213939, "strand": "-", "frame": 2, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGACAACTAAATACGGGCACCAAATTCAAATCGTTGGTGATGACTTAACAGTTACAAACCCTAAATTATTAGCGCGTGCTATTAAAGAAAAAGCAATGAATGCAATTTTAATTAAATTAAACCAAATTGGTTCATTAACAGAAACAATTGATGCTATTAACATGGCTCAAAAAGCTGGAATGGCTTGTGTTGTTTCACACCGTTCAGGTGAAACAGAAGACACAACATTAGCTGATGTTGCTGTTGCATTAAACACAGGACAAATCAAAACTGGATCAATGTCACGTACAGATAGAATTGCAAAATACAATAGATTATTAGCTATTGAAGAAGAATTAGATGGTGCTGATGAATTTGAAGGTCGTGAAGCATTCTACAACATTAAATAA", "aa": "MTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK", "aa_hexdigest": "5b4f7b6538a069aadc8a2cdbe95c8244", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 1.0, "subject_cov": 0.8376623376623377, "identity": 0.628, "score": 164.0, "evalue": 1.48e-49, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "IGIGPGHMMC_431", "locus": "GCA000148625_00397" }, { "type": "cds", "sequence": "contig_1", "start": 213955, "stop": 214710, "strand": "-", "frame": 2, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_C4XEI3" ], "nt": "ATGACCGCAGTTGATCATGTTAACAAAGATATTGCACCTAAATTATTAGGAATGGAAGTTACAGATCAACGTGCAATCGACATGAAAATGATTAAATTAGATGGCACAGCAACAAAATCAAAATTTGGTGCTAACGCTATCTTAGGAGTTTCATTAGCAGTAGCTAGAGCAGCCGCAGATGAATTACAACTTCCTTTATATAGATACATTGGTGGTACAAATGCCCACGTATTACCATTACCTATGCTAAATGTTATGAATGGTGGAGCTCACGCATCAAACACAGTTGACTTCCAAGAATTCATGATTATGCCAGTTGGTGCTAAAAGCATTCGTGAAGCATTACAAATGGCAAACAAAGTATTCCATAACTTAGCAAAATTATTAAAAGCTGCAGGACACGGAACACAAGTTGGTGATGAAGGTGGATTTGCACCTGACTGCAAAAGTCATGAAGAAGTTCTTGACTACTTAGTTCAAGCTATTAAGGCTGCAGGTTACAAACCAGCTACAAGCGGTAAAGATGCTGTTGCTATTGCAATGGATGCTGCTTCAAGTGAAATTTACGACGAAAAAAGCAAAAAATATGTATTCAAAAAACTTAAAAAAGCTATTGAAGCTAAACACCCAGGTTTTGAAAAACTTACAAATGTTAAACTTGAATACACAACAGATGAAATGATTGAATACTATGGTACTTTAATTGCTAAATACCCAATTATCTCAATCGAAGATGGTTTAGCAGAAGATGACTGA", "aa": "MTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDD", "aa_hexdigest": "7db3fce6604d83ecc25876a608756bf6", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 213652, "stop": 214932, "observations": { "mutation": [ 213957, 214731, 214839 ], "stop": [ 213957, 214731, 214839 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI3", "query_cov": 1.0, "subject_cov": 0.5456521739130434, "identity": 1.0, "score": 487.0, "evalue": 1.52e-170, "gene_start": 1, "gene_end": 251, "reference_start": 75, "reference_end": 325, "reference_sequence": "MKLRRKMSAIYKIHSREILDSRGNPTVQVEVWTEAGGYGLANVPSGASTGSKEALELRDQGTKYEKNWFGGKGVMTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDDWAGFTKMTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK" }, "paralog": false, "identity": 0.9906103286384976, "score": 819.0, "evalue": 6.19e-304, "description": "Internal stop codon at 213957, 214731, 214839. Nonsense mutation around 213957, 214731, 214839.", "psc": { "uniref90_id": "UniRef90_C4XEI3", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI3", "EC:4.2.1.11", "UniRef:UniRef50_P75189", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" } }, "id": "IGIGPGHMMC_432", "locus": "GCA000148625_00398" }, { "type": "cds", "sequence": "contig_1", "start": 215209, "stop": 215637, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGA", "aa": "MKKLLTFGMKKENQDIDSAFSRPLQFISNKVIAKLGVGSHLINDYTVTALGHAFCDSIVESEKNNKEFKIFLSNDNTFHALLYTNILARIFDEKGFKSYIFEKNSSYPSVLKNLAAKDDDCDVIISVESFKGLKNIMQISFN", "aa_hexdigest": "2b03d5f235cc455860a869c3ad8112e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16085.282099999982, "isoelectric_point": 7.737003135681152 }, "id": "IGIGPGHMMC_433", "locus": "GCA000148625_00399" }, { "type": "cds", "sequence": "contig_1", "start": 215689, "stop": 215982, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGA", "aa": "MNSVNYLSIDIPNQGISFKYNHSPLNYNREILKRYSPLISTFKKQNLKFGVDISQLSTANFYYDTLNRLQIDYFTHNRKKDAYIINANNPDCLKKIF", "aa_hexdigest": "c189ee9498c31e6d549ab6f99fd898df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11473.931199999983, "isoelectric_point": 9.519159889221193 }, "id": "IGIGPGHMMC_434", "locus": "GCA000148625_00400" }, { "type": "cds", "sequence": "contig_1", "start": 216379, "stop": 216822, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACAAATGTTGTAATTTTGGAAAGAGAACTTATGGATGGAATTTTAGAGTTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGA", "aa": "MLELMRMIAFYKEKSLTLYEVLQQIYDKYGLHHVITKTFNLDDEMASRFIKRILKSETIGNHKIVNFKEYKNATIVNNNIHLKLSFEGGNSITVKYSIIEKEITIDCEAIAKTNSQEDRTNVVILERELMDGILELKEDFKIRKITP", "aa_hexdigest": "1f67790d191061df217ae854bdf05d66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17229.84289999999, "isoelectric_point": 6.914940834045409 }, "id": "IGIGPGHMMC_435", "locus": "GCA000148625_00401" }, { "type": "cds", "sequence": "contig_1", "start": 216850, "stop": 217092, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTTAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGA", "aa": "MTFIGVIIFLYFSIYNFKDDSFFGANGDMGEMFKRMGIVIFKDHKTQFAFLSIVLSFFIWTLFNAIIFKRLLDFQGQKVK", "aa_hexdigest": "be23b2457f79a1601612a08498fcf622", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9455.13379999999, "isoelectric_point": 9.455400657653808 }, "id": "IGIGPGHMMC_436", "locus": "GCA000148625_00402" }, { "type": "cds", "sequence": "contig_1", "start": 217945, "stop": 218145, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAG", "aa": "MLVFIGEKRVDLYQIKEKNLRLIQNDNMSATTRTKTRFYKISFTIYTIIIIALAITILCPLMGSYK", "aa_hexdigest": "a44c1c53148b18455fec6649fea04e5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7718.2154999999975, "isoelectric_point": 9.841888618469238 }, "id": "IGIGPGHMMC_437", "locus": "GCA000148625_00403" }, { "type": "cds", "sequence": "contig_1", "start": 218376, "stop": 218468, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGCTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAAATATTACATGGCAAAACAAGATTTTAA", "aa": "MKGCTSVGFSAKIQFINKMKGKILHGKTRF", "aa_hexdigest": "9a75cfd299697de377da51b0b6085d36", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3357.0691000000015, "isoelectric_point": 10.685650825500488 }, "id": "IGIGPGHMMC_438", "locus": "GCA000148625_00404" }, { "type": "cds", "sequence": "contig_1", "start": 218449, "stop": 219048, "strand": "+", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "nt": "ATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGA", "aa": "MAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDT", "aa_hexdigest": "f272dd38a5d740934d6073baeb4b73d8", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AF59E5", "query_cov": 0.9798994974874372, "subject_cov": 0.8297872340425532, "identity": 0.836, "score": 335.0, "evalue": 9.45e-115, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI0021AF59E5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF59E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "product": "GTP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_439", "locus": "GCA000148625_00405" }, { "type": "cds", "sequence": "contig_1", "start": 219088, "stop": 219636, "strand": "+", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "GO:0003746", "GO:0005525", "GO:0006414", "SO:0001217", "UniRef:UniRef50_O83217", "UniRef:UniRef90_C4XEI5" ], "nt": "ATGGCTGTTGAAGACGTTTTCACAATTACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACAGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGCACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAA", "aa": "MAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK", "aa_hexdigest": "ae0171f1ba10dc3f8e3ac9906b75c5a6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00211B1E30", "query_cov": 1.0, "subject_cov": 0.9479166666666666, "identity": 0.7090000000000001, "score": 270.0, "evalue": 2.93e-90, "valid": false, "product": "EF-Tu/IF-2/RF-3 family GTPase", "uniref50_id": "UniRef50_A0A0A8KZ15" }, "genes": [ "tuf" ], "truncated": "5-prime", "pseudogene": { "start": 218788, "stop": 219636, "observations": { "mutation": [ 219046 ], "stop": [ 219046 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI5", "query_cov": 1.0, "subject_cov": 0.41935483870967744, "identity": 1.0, "score": 365.0, "evalue": 3.78e-124, "gene_start": 1, "gene_end": 182, "reference_start": 253, "reference_end": 434, "reference_sequence": "MFIKVILVNSMDRMQMKDCTSVGFSAKIQFINKMKGKYYMAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDTWIQTPVKEFDKPFLMAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK" }, "paralog": false, "identity": 0.9964539007092199, "score": 556.0, "evalue": 7.28e-202, "description": "Internal stop codon at 219046. Nonsense mutation around 219046.", "psc": { "uniref90_id": "UniRef90_C4XEI5", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_O83217", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI5", "UniRef:UniRef50_O83217", "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_O83217", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O83217" ], "product": "Elongation factor Tu" } }, "id": "IGIGPGHMMC_440", "locus": "GCA000148625_00406" }, { "type": "cds", "sequence": "contig_1", "start": 219761, "stop": 220162, "strand": "+", "frame": 2, "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "db_xrefs": [ "GO:0002949", "RefSeq:WP_013354457.1", "SO:0001217", "UniParc:UPI0001E33007", "UniRef:UniRef100_UPI0001E33007", "UniRef:UniRef50_A0A449B9H2", "UniRef:UniRef90_C4XEI6" ], "nt": "ATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGAAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAACATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCACATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAA", "aa": "MKNLKTFITRDVKEVKKVAQYVLDHLTKSKLVLMNGDLGAGKTTLTKEIAKLLNIDEVITSPTFNYMKVYDGLVHIDAYNLKGDISEFEDYFEDNVVVIEWANRIKHYYKNYLDINITLDKDNNHVFTIKEAK", "aa_hexdigest": "ef46bdf1649c7750053d85c95f8b8c4c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E33007", "ncbi_nrp_id": "WP_013354457.1", "uniref100_id": "UniRef100_UPI0001E33007", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33007", "RefSeq:WP_013354457.1", "UniRef:UniRef100_UPI0001E33007" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33007", "uniref90_id": "UniRef90_C4XEI6" }, "psc": { "uniref90_id": "UniRef90_C4XEI6", "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "uniref50_id": "UniRef50_A0A449B9H2", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B9H2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B9H2" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" }, "genes": [ "tsaE" ], "id": "IGIGPGHMMC_441", "locus": "GCA000148625_00407" }, { "type": "cds", "sequence": "contig_1", "start": 220162, "stop": 220722, "strand": "+", "frame": 1, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_013354458.1", "SO:0001217", "UniParc:UPI0001E33008", "UniRef:UniRef100_C4XEI7", "UniRef:UniRef50_N9VF08", "UniRef:UniRef90_C4XEI7" ], "nt": "ATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAA", "aa": "MKLYLDTANEDFVLALLNDKHQVIAQKVLESYPKKVELIPQLTEEILKENNFKIQDFDTFYINIGPGFFTGVRIALVYLRTIVLITKANIKTISTMQILAKQNRRKRSFKINAQGNKYYLYETNKNSAFNYKNIKVETGTLKAYDKVNYFDFLSCFIDYLDEFKSYKDVMDIEPYYIKMPQIGAKK", "aa_hexdigest": "7a8f44009b96a1347d13d10bc328d8dc", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33008", "ncbi_nrp_id": "WP_013354458.1", "uniref100_id": "UniRef100_C4XEI7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33008", "RefSeq:WP_013354458.1", "UniRef:UniRef100_C4XEI7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI7", "uniref90_id": "UniRef90_C4XEI7" }, "psc": { "uniref90_id": "UniRef90_C4XEI7", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_N9VF08", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF08" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "IGIGPGHMMC_442", "locus": "GCA000148625_00408" }, { "type": "cds", "sequence": "contig_1", "start": 220722, "stop": 221648, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q4A734", "UniRef:UniRef90_C4XEI8" ], "nt": "ATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTATTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCCTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCGGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACATTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGCTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGCTAATCATTCTGAAGTTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAAATTAAATGA", "aa": "MRILGIETSHDDTSIALLEDGKVLALKTISQIDIFKEFGGTIPEISSREHVKNINLIEKIFLQEFDLNTIDYIAYTQKPGLIGTLQIGFLFASALALALNKPLLPINHLEGHFYSAAIKQEIKYPALSLLVSGGHTQLMLVKSPFDIQIIGQTLDDAVGEAYDKVASRLGFGFPGGPIIDKMYQDYKGDFVALTLPHTDGLYDFSFSGLKSQVLNHYNNALQRKKALNKEQLAVSFQETAVTYLINKTKLALANHSEVKTLVLAGGVSANSELRKRFLKLSTKAIVPNLKYSTDNGAMIAMCAYCKIK", "aa_hexdigest": "c4dd8a6ecb76ab5326075a7439d3adc9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEI8", "query_cov": 1.0, "subject_cov": 0.9903536977491961, "identity": 0.99, "score": 600.0, "evalue": 1.74e-216, "valid": true, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q4A734", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A734", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A734" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" }, "genes": [ "tsaD" ], "id": "IGIGPGHMMC_443", "locus": "GCA000148625_00409" }, { "type": "cds", "sequence": "contig_1", "start": 221923, "stop": 222102, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCATTCAATCTCTCCATTAACCCTTGATGAACATATTGAAAAAATATATGATGCATTTATAAAATTTATAAATGAAAAGTACAACAATGACACCACTACTTTTAGTGCTGAAAATTTCGCAAAAGAATTTAATGAAGTAAATGATGGTAATGGAGTTAAATTAGAGAATGTTTAG", "aa": "MAHSISPLTLDEHIEKIYDAFIKFINEKYNNDTTTFSAENFAKEFNEVNDGNGVKLENV", "aa_hexdigest": "469031b184769c8c4abd8857365a8739", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6767.3659000000025, "isoelectric_point": 4.562602424621583 }, "id": "IGIGPGHMMC_444", "locus": "GCA000148625_00410" }, { "type": "cds", "sequence": "contig_1", "start": 222316, "stop": 222462, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAAACTTATCTTTAAAGAACCGCGTTATTTTGAAGATGCAACTCTAAGTGTAGATAATAATAACAAATATAAAATAAGTTTTGGAAATACCAAAATGCATGCTGCTGATTTTGAAAAAATAATTAACATTAATGATATTTGA", "aa": "MPKLIFKEPRYFEDATLSVDNNNKYKISFGNTKMHAADFEKIININDI", "aa_hexdigest": "57638aadb113e50bf9359a2750029b05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5635.3845, "isoelectric_point": 6.533323860168457 }, "id": "IGIGPGHMMC_445", "locus": "GCA000148625_00411" }, { "type": "cds", "sequence": "contig_1", "start": 222613, "stop": 222951, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAATGAATTCTTGAATAAATCAATATTTGAATCTACTTTATCAAACGAAAACTTTGAATTTGAAGTTTCAAATCAAAATAATAAACCACAAATTACTTTAAAAATCAAGAAATTTAGACATTTTTCAAAAAAATATTTCAATTGGGACGAAATTGAAAACAATAATTACAACAATGTGTTATATGCAATCACAAAGAAATCAGAAATTATTTTTGATAATGTAGATAATGAATGTGAAACGTTGCTTGACTTAGCAATCAACGATTATATTGAAAAAAGAAAAAATGAAATACTTTTATACTCAGAACGTATGAAAACAACTGATTCATGA", "aa": "MFDNEFLNKSIFESTLSNENFEFEVSNQNNKPQITLKIKKFRHFSKKYFNWDEIENNNYNNVLYAITKKSEIIFDNVDNECETLLDLAINDYIEKRKNEILLYSERMKTTDS", "aa_hexdigest": "8d4c4933ad0dc1aac0b5485f225cf56c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13523.950899999982, "isoelectric_point": 4.929839134216308 }, "id": "IGIGPGHMMC_446", "locus": "GCA000148625_00412" }, { "type": "cds", "sequence": "contig_1", "start": 223273, "stop": 223545, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAGAAAAATCATTTGGCTAAAAATATTAGTAATACAGTACCAAATCTATCAAGTGAAACTAATATATCAACTTATGAAAAAAATGATGAAAAAATTATAAGAGTTGAAGATATTAAATTAAATGATACAGGAGCAGAAGATCCAAATACTCATGAAAAAGAATTTGAAAGATTTTTCTTGATTTCTTTAAATGAAAGAAAAATTCGTTCTAATGGTGAAAAAACTGGAATTAAAGAATTAAAATATGATGAAAAAATTACTAAATGA", "aa": "MIKKNHLAKNISNTVPNLSSETNISTYEKNDEKIIRVEDIKLNDTGAEDPNTHEKEFERFFLISLNERKIRSNGEKTGIKELKYDEKITK", "aa_hexdigest": "c4a771f85a435bd438e01046707582df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10494.68619999999, "isoelectric_point": 6.744082832336426 }, "id": "IGIGPGHMMC_447", "locus": "GCA000148625_00413" }, { "type": "cds", "sequence": "contig_1", "start": 224401, "stop": 225195, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGGATTTAAACAATGCCAATACTTTAAATATAGTTAAGGAATATTGTGGAATAAAAGATGAAAAAAATATAAATATTGTATTTCAAACTTTTATTAAAAAATACATTTCAAATTCAAAATTAGAATTCATAAGTCGTTTTGAAAATATACTTTCAAATGCGGATTACAACAAAAAAACATTTTATTATGATTCAGAAGTTGCAAAATTATCAGTTGATAAGAAATTCGATAAAATAACAAATTACTTTTACGGTACTAATTCAAATTTTTTTGATTCATTTATAAATGATAAATATACATTTAATATTTCATTAAAAAATATTGGTTTTAAAAAAGATAATACAACATATCTCGACATTAATAATTTAAATAAAGAAATAAGCGTAACATATGATATTGTAAACAAAATTTATGAATTTAATACAATAGGTTTTTTTGCTACAAACAATATCAACAAAATAGTTGATGAATTATTTAAAGAAAAAGATAATATCTTTAAATATCCTTTACAAGATTTAGATTATGAAAAATGTAGAAAAGAAATAATTGAAAAAATAAAAAATGATATTCAAAGTAAATTGACTAATGCTTATATTTCTGAAATTGATAATGAATGTGATTTATCAAAATTGAATATAAAAGCTGGTGTAAATTATCCTTCAGAATACAAAGATAAAAATATAGAAATTGTAACTCTTGAATCAAAAACATTAAAACCATCAGGTGTTTCTGACTTTGATAGTTTTAAAAAATATAACGAATTAAAAAATTATTTAAATAGTTCGATTTGA", "aa": "MPDLNNANTLNIVKEYCGIKDEKNINIVFQTFIKKYISNSKLEFISRFENILSNADYNKKTFYYDSEVAKLSVDKKFDKITNYFYGTNSNFFDSFINDKYTFNISLKNIGFKKDNTTYLDINNLNKEISVTYDIVNKIYEFNTIGFFATNNINKIVDELFKEKDNIFKYPLQDLDYEKCRKEIIEKIKNDIQSKLTNAYISEIDNECDLSKLNIKAGVNYPSEYKDKNIEIVTLESKTLKPSGVSDFDSFKKYNELKNYLNSSI", "aa_hexdigest": "8e6119a6635980c69c115b71b2737a3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30974.611400000045, "isoelectric_point": 5.829770851135254 }, "id": "IGIGPGHMMC_448", "locus": "GCA000148625_00414" }, { "type": "cds", "sequence": "contig_1", "start": 225349, "stop": 225570, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTGCAAAATTTTTAAGTATTAACTTTAATGGTCAAACACCATTTACAAAAGAAATATTTAAAAAAAATAATGAAGAATTTAAATTAAATTATTATGACAAACTAGGATACTTAACAAATAAACTTCAAAAAGATGATAATTATTTTAGTGATTTTATTTGTAACCTAAATAGTTTTGGTCTATTCTTTGTAGATTCAAATAAAGCAACTAATTGA", "aa": "MSFAKFLSINFNGQTPFTKEIFKKNNEEFKLNYYDKLGYLTNKLQKDDNYFSDFICNLNSFGLFFVDSNKATN", "aa_hexdigest": "800390bf71359bb1de6d63477cda288a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8632.609499999997, "isoelectric_point": 7.7970159530639656 }, "id": "IGIGPGHMMC_449", "locus": "GCA000148625_00415" }, { "type": "cds", "sequence": "contig_1", "start": 226216, "stop": 226740, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAATAAAAAAACAAACATTGATTATCCATTTTATTGTAGTGAACTATTAAAAAATTCTGATTTTAATAATTCGACAGTTTTTGGCAATGAATCAAATAAAGCTGAATTAAAAAATACCAACAATACTTTTAAAATTAGATATGATATTTCTCCAAGAATTAAGATTTTTGATATTTCAATCGATAAAACTAATAAAGAACATGCCGAAGAAGTTGAGTCATTATTAAAGAATAATAAATTAGATGACATTTTCAATAGAAAAATATTTTCAAATAATATTAAAAATGAATTATTACCTCCTGAAAAATTTAGTACTATTAAAGGTGAACCAGGTTGTGATGAGTTTATTGAATCTTACTCAGAAAATAAAAAATATAATACTAAACTAATTTCTTCTAATCCAAATACACCATTGATTAACTTATATTTCATTTCTGAAGACCAAAAAACTTTATACAAAGTACCTGAAAAAGTAGATAAAAATGACCTTGTTTATAATTTGTTTAAAGAATATTGA", "aa": "MENNKKTNIDYPFYCSELLKNSDFNNSTVFGNESNKAELKNTNNTFKIRYDISPRIKIFDISIDKTNKEHAEEVESLLKNNKLDDIFNRKIFSNNIKNELLPPEKFSTIKGEPGCDEFIESYSENKKYNTKLISSNPNTPLINLYFISEDQKTLYKVPEKVDKNDLVYNLFKEY", "aa_hexdigest": "9c710547700f4c05a9c03d0a7c309ff0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20434.69460000001, "isoelectric_point": 5.627708244323729 }, "id": "IGIGPGHMMC_450", "locus": "GCA000148625_00416" }, { "type": "cds", "sequence": "contig_1", "start": 226801, "stop": 226929, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAATTGAGCTTGTTAAAGGTCAATGCAATTCAGAATATGATGTGGTTATTTTAAAAATAAACAACATTTATATAAGTTCAAATGATAAATCAATAGTTGAAAAAATAAAAAATGACATTTGA", "aa": "MPRIELVKGQCNSEYDVVILKINNIYISSNDKSIVEKIKNDI", "aa_hexdigest": "46fdcb4130c5689acf9d8b4fbef4e0ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4837.5699, "isoelectric_point": 6.057922554016113 }, "id": "IGIGPGHMMC_451", "locus": "GCA000148625_00417" }, { "type": "cds", "sequence": "contig_1", "start": 227335, "stop": 227442, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACAAAAGAAAAAAAGTTATAGCGACAGTGGCAACTGCACTCTCGTTAACAACACTTGGAGTAGTTAGTGCTGCTGCTTTTTACAATGTAAATAGCTGA", "aa": "MIKNKRKKVIATVATALSLTTLGVVSAAAFYNVNS", "aa_hexdigest": "9a5e590a97f90a0d09366438282a78dd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3681.3502, "isoelectric_point": 10.582501411437985 }, "id": "IGIGPGHMMC_452", "locus": "GCA000148625_00418" }, { "type": "cds", "sequence": "contig_1", "start": 227794, "stop": 227898, "strand": "+", "frame": 1, "gene": "ydbC", "product": "YdbC family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1", "UniRef:UniRef90_UPI000FF2E50B" ], "nt": "ATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGA", "aa": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVS", "aa_hexdigest": "172154335c0cd7455d67729fa2d6f02c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [ "ydbC" ], "truncated": "3-prime", "pseudogene": { "start": 227794, "stop": 228032, "observations": { "deletion": [ 227986 ], "mutation": [ 227896, 227932 ], "stop": [ 227896, 227932, 228010 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF2E50B", "query_cov": 1.0, "subject_cov": 0.43037974683544306, "identity": 1.0, "score": 70.5, "evalue": 3.25e-15, "gene_start": 1, "gene_end": 34, "reference_start": 1, "reference_end": 34, "reference_sequence": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVSWNEGEAKYDIRDWSEDHLRSSKGITLSLEEFKTIKSFDWQRASKN" }, "paralog": false, "identity": 0.9493670886075949, "score": 139.0, "evalue": 6.73e-45, "description": "Internal stop codon at 227896, 227932, 228010. Frameshift due to deletion around 227986. Nonsense mutation around 227896, 227932.", "psc": { "uniref90_id": "UniRef90_UPI000FF2E50B", "product": "YdbC family protein", "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF2E50B", "UniRef:UniRef50_A0A4P6MMX1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1" ], "product": "Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein" } }, "id": "IGIGPGHMMC_453", "locus": "GCA000148625_00419" }, { "type": "cds", "sequence": "contig_1", "start": 228039, "stop": 228836, "strand": "+", "frame": 3, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354463.1", "SO:0001217", "UniParc:UPI0001E33029", "UniRef:UniRef100_C4XEJ2", "UniRef:UniRef50_C4XEJ2", "UniRef:UniRef90_C4XEJ2" ], "nt": "ATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAA", "aa": "MILATKDDKEVILKFLNQEPIYNLLIVGDIMTYGVRTPYLFTYIIKEEEQIKAVILIFNKTVLIYDPEFKVSLKDLKSLIKKHSLINVNLSEKMYQHYQDEFENNGDKYKVHRQTIAILENRYTGDTSLAKKAIFTDSEPIVRSRFLIDEFVDFRGTYQQEVKSYKNALIKKVSTPFIIKDKQNRVASHANILISSDKASMIGGVFTLKEYRQKGYAIQVVGALCNHIFANKRVPMLFFDNPAAAQLYHKIGFKDFGKVFTIKIN", "aa_hexdigest": "811f442de7ab516ae9258b3f107138e7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33029", "ncbi_nrp_id": "WP_013354463.1", "uniref100_id": "UniRef100_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33029", "RefSeq:WP_013354463.1", "UniRef:UniRef100_C4XEJ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ2", "uniref90_id": "UniRef90_C4XEJ2" }, "psc": { "uniref90_id": "UniRef90_C4XEJ2", "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XEJ2" }, "pscc": { "uniref50_id": "UniRef50_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ2" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_454", "locus": "GCA000148625_00420" }, { "type": "cds", "sequence": "contig_1", "start": 228850, "stop": 229467, "strand": "+", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_A0A449BAE8", "UniRef:UniRef90_A0A3P8MDR8" ], "nt": "ATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGA", "aa": "MLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQEL", "aa_hexdigest": "53b37c338906263ae741a9d209ed6956", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ffh" ], "truncated": "3-prime", "pseudogene": { "start": 228832, "stop": 229770, "observations": { "mutation": [ 229465 ], "stop": [ 229465 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MDR8", "query_cov": 1.0, "subject_cov": 0.4466230936819172, "identity": 1.0, "score": 383.0, "evalue": 2.28e-130, "gene_start": 1, "gene_end": 205, "reference_start": 7, "reference_end": 211, "reference_sequence": "MNKGDNMLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQELWDLKKIANPSEIFFVADALSGQDILNVAETFNNKLKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA" }, "paralog": false, "identity": 0.9935897435897436, "score": 587.0, "evalue": 4.23e-213, "description": "Internal stop codon at 229465. Nonsense mutation around 229465.", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR8", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A449BAE8", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MDR8", "EC:3.6.5.4", "UniRef:UniRef50_A0A449BAE8", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAE8" ], "product": "Signal recognition particle protein" } }, "id": "IGIGPGHMMC_455", "locus": "GCA000148625_00421" }, { "type": "cds", "sequence": "contig_1", "start": 229567, "stop": 230211, "strand": "+", "frame": 1, "gene": null, "product": "Signal recognition particle protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "nt": "TTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAA", "aa": "MKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA", "aa_hexdigest": "3c1974398956a2d55a7827bd71c8d0d4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PFZ2", "query_cov": 0.9719626168224299, "subject_cov": 0.9285714285714286, "identity": 0.635, "score": 249.0, "evalue": 4.86e-81, "valid": false, "product": "Signal recognition particle protein", "uniref50_id": "UniRef50_A0A3B0PFZ2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PFZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "product": "Signal recognition particle protein" }, "genes": [], "id": "IGIGPGHMMC_456", "locus": "GCA000148625_00422" }, { "type": "cds", "sequence": "contig_1", "start": 230538, "stop": 230702, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGA", "aa": "MLLIALSLIFNKLHYVKYYGFLAIISGLLALVKVDFNFTQEEGKDIIFPIGIDN", "aa_hexdigest": "11aa76552efaa29230bbb8baa593fc89", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6133.240400000001, "isoelectric_point": 5.498115348815917 }, "id": "IGIGPGHMMC_457", "locus": "GCA000148625_00423" }, { "type": "cds", "sequence": "contig_1", "start": 231895, "stop": 232383, "strand": "+", "frame": 1, "gene": null, "product": "F0F1 ATP synthase subunit A", "db_xrefs": [ "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_C4XEJ6", "UniRef:UniRef90_C4XEJ6" ], "nt": "ATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGA", "aa": "MPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTG", "aa_hexdigest": "5f677a454876725bd33c4273e99f211d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 231814, "stop": 232653, "observations": { "mutation": [ 231883, 231889, 232381, 232387, 232417, 232522, 232627 ], "stop": [ 231883, 231889, 232381, 232387, 232417, 232522, 232627 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEJ6", "query_cov": 1.0, "subject_cov": 0.5806451612903226, "identity": 1.0, "score": 299.0, "evalue": 9.62e-101, "gene_start": 1, "gene_end": 162, "reference_start": 28, "reference_end": 189, "reference_sequence": "MKKTKKFKITRKGGQVEKLTQNLWKWNMPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTGWIWTQIPGLLGEWFFIGSLITPFLHMYFDMFGAIIQALVFCTLTTVYWSKEAVTNENKKQQDAQEKAESEKSVANVENKAVAWSNSSSVY" }, "paralog": false, "identity": 0.9713261648745519, "score": 512.0, "evalue": 3.82e-187, "description": "Internal stop codon at 231883, 231889, 232381, 232387, 232417, 232522, 232627. Nonsense mutation around 231883, 231889, 232381, 232387, 232417, 232522, 232627.", "psc": { "uniref90_id": "UniRef90_C4XEJ6", "product": "F0F1 ATP synthase subunit A", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEJ6", "EC:7.1.2.2", "UniRef:UniRef50_C4XEJ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ6" ], "product": "ATP synthase subunit a" } }, "id": "IGIGPGHMMC_458", "locus": "GCA000148625_00424" }, { "type": "cds", "sequence": "contig_1", "start": 232673, "stop": 232909, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354468.1", "SO:0001217", "UniParc:UPI0001E3302E", "UniRef:UniRef100_C4XEJ7", "UniRef:UniRef50_C4XEJ7", "UniRef:UniRef90_C4XEJ7" ], "nt": "ATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAA", "aa": "MTQGGGLVAIGIGIAMLGSFGTGLGQGLAAGKATEAVGRNPEAAAKIRSMLLIGQGVAESSAIYCLVVAFILAFAFTK", "aa_hexdigest": "c899121cf9fca3778600cf0a4ba77d9a", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3302E", "ncbi_nrp_id": "WP_013354468.1", "uniref100_id": "UniRef100_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302E", "RefSeq:WP_013354468.1", "UniRef:UniRef100_C4XEJ7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ7", "uniref90_id": "UniRef90_C4XEJ7" }, "psc": { "uniref90_id": "UniRef90_C4XEJ7", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ7", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ7" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "IGIGPGHMMC_459", "locus": "GCA000148625_00425" }, { "type": "cds", "sequence": "contig_1", "start": 232917, "stop": 233441, "strand": "+", "frame": 3, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354469.1", "SO:0001217", "UniParc:UPI0001E3302F", "UniRef:UniRef100_C4XEJ8", "UniRef:UniRef50_C4XEJ8", "UniRef:UniRef90_C4XEJ8" ], "nt": "ATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAA", "aa": "MHTINKSLGTKIEEELNGLFPSVPMLVATLVALAIIILILFFLLYKPIKKSIKARQDYIQSNIDQAKATNDLSKNKLKEANDKLILAHEEANNLVKNAKIRAEKVIISYTAKAKMESKRIIEEAELDIEQQKDSLMEDSKNQIAKAATELSRQILKKEVSKKTESEIIDKFLES", "aa_hexdigest": "36b825b4af6c6c4ced67b7de4879d183", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3302F", "ncbi_nrp_id": "WP_013354469.1", "uniref100_id": "UniRef100_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302F", "RefSeq:WP_013354469.1", "UniRef:UniRef100_C4XEJ8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ8", "uniref90_id": "UniRef90_C4XEJ8" }, "psc": { "uniref90_id": "UniRef90_C4XEJ8", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ8", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ8" ], "product": "ATP synthase subunit b" }, "genes": [ "atpF" ], "id": "IGIGPGHMMC_460", "locus": "GCA000148625_00426" }, { "type": "cds", "sequence": "contig_1", "start": 233483, "stop": 234040, "strand": "+", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354470.1", "SO:0001217", "UniParc:UPI0001A8C1A4", "UniRef:UniRef100_C4XEJ9", "UniRef:UniRef50_C4XEJ9", "UniRef:UniRef90_C4XEJ9" ], "nt": "ATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAG", "aa": "MYSKANPEGYALALFELTKEEKDMKAINEVVVSLYNTVVESDKLVMILNDQALEKNKKFDYIDKIFKNISSSKLIVNFLKVLVERNAATLLRRSLEHYLKLSNEFLNIRFALITSAFEISEDKLKKIKQKLEKKYNTEIILKHQIDESLISGFKIKMDSLVIEQNFKKDLQELNTILTKKGGLDG", "aa_hexdigest": "cfc6a7b40b325693d6bb4832d6c95216", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A4", "ncbi_nrp_id": "WP_013354470.1", "uniref100_id": "UniRef100_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A4", "RefSeq:WP_013354470.1", "UniRef:UniRef100_C4XEJ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ9", "uniref90_id": "UniRef90_C4XEJ9" }, "psc": { "uniref90_id": "UniRef90_C4XEJ9", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ9" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "IGIGPGHMMC_461", "locus": "GCA000148625_00427" }, { "type": "cds", "sequence": "contig_1", "start": 234033, "stop": 234779, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGA", "aa": "MASRTDDISAIIKDRIRNFDSKVDYSEVGTIITIGDGIALVKGLDNAKNGEIILFKNNIYGLVLNLEEEVVGVTIFGNANSLSEGDTCKRSGEVISIPVGDKLLGRVVNALGQPIDGKGKINTTVKKEIFKVAPGVMTRQEVNQPLETGIIAIDSMIPIGKGQRELIIGDRQTGKTAIAIDTIINQKGKNVNCIYVAIGQKNSTVAQIVKKLEDTGAMEYTTVVVSGASELAPQQYIAPYSGVTIAEE", "aa_hexdigest": "af621f36fcb4a40cce4bae0cd82dd14c", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.967741935483871, "subject_cov": 0.9411764705882353, "identity": 0.604, "score": 302.0, "evalue": 1.09e-100, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "IGIGPGHMMC_462", "locus": "GCA000148625_00428" }, { "type": "cds", "sequence": "contig_1", "start": 234780, "stop": 235619, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "ATGGCCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAA", "aa": "MAKGKDCLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSQLLERAARVTKEYGGGSITALPIIETQQGDISAYIPTNVISITDGQIFTKENLFNSGQRPAVDVGFSVSRVGSAAQIKAMKKVAGSLKLELAQYNEMLAFAQFGSDLDDSTKAILQHGAKVYEILKQEQYAPISQIAQVAILLGVKERIINPLPAKYIALYREEVIKYIESDPEGVSIALEIHNNNNEFSDVHFETITKAILKIVKRIIASIPNYDPSDYLPMPEKYSEKSKK", "aa_hexdigest": "638d954f06212560349a21f44ec448d5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.8853046594982079, "subject_cov": 0.8487972508591065, "identity": 0.5870000000000001, "score": 277.0, "evalue": 5.93e-90, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "IGIGPGHMMC_463", "locus": "GCA000148625_00429" }, { "type": "cds", "sequence": "contig_1", "start": 235619, "stop": 236491, "strand": "+", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "RefSeq:WP_013354472.1", "SO:0001217", "UniParc:UPI0001E33031", "UniRef:UniRef100_C4XEK1", "UniRef:UniRef50_F9UJQ6", "UniRef:UniRef90_C4XEK1" ], "nt": "ATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAG", "aa": "MAGTQAIKSRISTVESIRKITHAMELVAASKLRRARIEFENVKAYNEVIKETFRSILNHLSPTEIKHLFPIHNIESRMFIIVTSDLGLAGSYNSNIIKFAKNTIKKTDKVILIGRKAISSLASLYENQIVTQFNCDSSEKDYNIVHDIMTIVFELYNRKQVNSINVIYNKYMNNLVQQETCDQVFPFKFDEDKKQKHAEKHKESLNSIIEFEPSAQQVLSESIPLFIDAELYLAYTSAKLSEMAARRSAMESATDNADELIDNLNLEFNRKRQSSITQELNEIVAGADAV", "aa_hexdigest": "bf8dd84f316fdf6e87e135e633de846f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E33031", "ncbi_nrp_id": "WP_013354472.1", "uniref100_id": "UniRef100_C4XEK1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33031", "RefSeq:WP_013354472.1", "UniRef:UniRef100_C4XEK1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK1", "uniref90_id": "UniRef90_C4XEK1" }, "psc": { "uniref90_id": "UniRef90_C4XEK1", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_F9UJQ6", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_F9UJQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UJQ6" ], "product": "ATP synthase gamma chain" }, "genes": [ "atpG" ], "id": "IGIGPGHMMC_464", "locus": "GCA000148625_00430" }, { "type": "cds", "sequence": "contig_1", "start": 236501, "stop": 238009, "strand": "+", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354473.1", "SO:0001217", "UniParc:UPI0001A8C1A7", "UniRef:UniRef100_C4XEK2", "UniRef:UniRef50_Q1AVH9", "UniRef:UniRef90_C4XEK2" ], "nt": "ATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAA", "aa": "MAVKKTTTKKVTSAKKSTTKVTITKKLTEKINKNNVGKIIQVLGPVVDVKFNKGFLPSLLNALKVYSNDEVYTLEVAQHIGDDTVRTISMVSTNGLARGLDVIDTGAPISVPVGDKVLGRMFDVLGQPIDLQEAPKAPLMPIHASAPSYEEQKTVSEVLETGIKVIDLLIPYAKGGKIGLFGGAGVGKTVLVQELINNIATEHGGLSVFAGVGERTREGNDLYHEMKAAGVLDKTALVFGQMNEPPGARMRVALTGLTMAEYFRDKENQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERITSTRRGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLDRNIAALGIYPAVDPLESNSRLLDPLVVGQEHYEVAHGVVNILQRFKELQDIIAILGMGELSEEDKLLVARARRIRNFLSQPFNVAEKFSGKKGKYVPISDTIRSFKDILEGKYDDCPEEFFLYVGSIDEVEAKYKEYLAKKEANKN", "aa_hexdigest": "8a9106edcfa70595edde033d65814494", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1A7", "ncbi_nrp_id": "WP_013354473.1", "uniref100_id": "UniRef100_C4XEK2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A7", "RefSeq:WP_013354473.1", "UniRef:UniRef100_C4XEK2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK2", "uniref90_id": "UniRef90_C4XEK2" }, "psc": { "uniref90_id": "UniRef90_C4XEK2", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1AVH9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1AVH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1AVH9" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "IGIGPGHMMC_465", "locus": "GCA000148625_00431" }, { "type": "cds", "sequence": "contig_1", "start": 238053, "stop": 238478, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase epsilon chain", "db_xrefs": [ "RefSeq:WP_013354474.1", "SO:0001217", "UniParc:UPI0001A8C1A8", "UniRef:UniRef100_C4XEK3", "UniRef:UniRef50_A0A3P8MDS1", "UniRef:UniRef90_C4XEK3" ], "nt": "ATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAA", "aa": "MLNKKTTHLKITTLEEIFFEGDVRSVTLRTKTGGAICLQPSRTPFFSTIDICELTINSPKDNDFRVCSIGGGLVYADATNINIITDDIIFGKNIDIKRAKIDRDFAMAQLEKFRDTKEEVQYEIKLRKALNRIDVYNRNIK", "aa_hexdigest": "a1ab310e0a3c7964d2ecff8d80619e81", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A8", "ncbi_nrp_id": "WP_013354474.1", "uniref100_id": "UniRef100_C4XEK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A8", "RefSeq:WP_013354474.1", "UniRef:UniRef100_C4XEK3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK3", "uniref90_id": "UniRef90_C4XEK3" }, "psc": { "uniref90_id": "UniRef90_C4XEK3", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A3P8MDS1" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDS1" ], "product": "ATP synthase epsilon chain" }, "genes": [], "id": "IGIGPGHMMC_466", "locus": "GCA000148625_00432" }, { "type": "tRNA", "sequence": "contig_1", "start": 238583, "stop": 238656, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "pseudogene": true, "score": 44.9, "nt": "GCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 238615, 238617 ], "id": "IGIGPGHMMC_6", "locus": "GCA000148625_00433" }, { "type": "cds", "sequence": "contig_1", "start": 239439, "stop": 239531, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGA", "aa": "MNRNNNLNNYMELVTLGKEYEDRSNRANLA", "aa_hexdigest": "4fce2eaae5b6407c283ff1ba7c0d9ef4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.8830000000007, "isoelectric_point": 6.013872337341308 }, "id": "IGIGPGHMMC_467", "locus": "GCA000148625_00434" }, { "type": "cds", "sequence": "contig_1", "start": 239820, "stop": 240107, "strand": "-", "frame": 3, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354476.1", "SO:0001217", "UniParc:UPI0001E33033", "UniRef:UniRef100_C4XEK6", "UniRef:UniRef50_C4XEK6", "UniRef:UniRef90_C4XEK6" ], "nt": "ATGAGTAGAAAAAATAAGAGAAACTATAAAAAAATAATCAATTATGATAAAGCAGAATCAAATGTCATCGACTTACATGGGTTAAATATTTATGAAGCAGTAGCAGAAATTCAATTTGCAATTGCTAATGCTAAAGATAATTCAGAATTTAGCAATAATTACATATTAGCCATTATCGGCAAAGGAACTGAAACTATAAAAGCATATATTGAAGAATTTTTAAGAGATTCAAACTATGAATATATGTTCAATGAGGACTATACCACTATCAAAATATATATTGCTTAG", "aa": "MSRKNKRNYKKIINYDKAESNVIDLHGLNIYEAVAEIQFAIANAKDNSEFSNNYILAIIGKGTETIKAYIEEFLRDSNYEYMFNEDYTTIKIYIA", "aa_hexdigest": "ffc184f34b9af43a1aa7957a0ec31ef5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33033", "ncbi_nrp_id": "WP_013354476.1", "uniref100_id": "UniRef100_C4XEK6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33033", "RefSeq:WP_013354476.1", "UniRef:UniRef100_C4XEK6" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK6", "uniref90_id": "UniRef90_C4XEK6" }, "psc": { "uniref90_id": "UniRef90_C4XEK6", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEK6" }, "pscc": { "uniref50_id": "UniRef50_C4XEK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK6" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_468", "locus": "GCA000148625_00435" }, { "type": "cds", "sequence": "contig_1", "start": 240461, "stop": 240742, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013354477.1", "SO:0001217", "UniParc:UPI0001E33034", "UniRef:UniRef100_UPI0001E33034", "UniRef:UniRef50_C4XEK7", "UniRef:UniRef90_C4XEK7" ], "nt": "TTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MYNEPQIPDFLERGSFLCNSNLKKKKGRKSKINLDAQKTTNFKINIENKIEDKDLLIKQLKEENKILKLENAIAKKMSALVQLKDSLTKKNSK", "aa_hexdigest": "1d2463fd289f62c22a2d77c16b95063d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33034", "ncbi_nrp_id": "WP_013354477.1", "uniref100_id": "UniRef100_UPI0001E33034", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33034", "RefSeq:WP_013354477.1", "UniRef:UniRef100_UPI0001E33034" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33034", "uniref90_id": "UniRef90_C4XEK7" }, "psc": { "uniref90_id": "UniRef90_C4XEK7", "product": "Transposase", "uniref50_id": "UniRef50_C4XEK7" }, "pscc": { "uniref50_id": "UniRef50_C4XEK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK7" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_469", "locus": "GCA000148625_00436" }, { "type": "cds", "sequence": "contig_1", "start": 240808, "stop": 241164, "strand": "+", "frame": 1, "gene": null, "product": "Transposase, IS861", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAACAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFGLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKSLNLFGKTLRRKNKYSSFKGDAHKNIPNLLLEKENITKDFFRHIRNFSNEKYLDI", "aa_hexdigest": "fa97fb12a5fd9cecf486eca2567fab25", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AEN5", "query_cov": 0.8135593220338984, "subject_cov": 0.8727272727272727, "identity": 0.51, "score": 82.8, "evalue": 2.81e-18, "valid": false, "product": "Transposase, IS861", "uniref50_id": "UniRef50_A0A449AEP7" }, "pscc": { "uniref50_id": "UniRef50_A0A449AEP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "product": "Transposase, IS861" }, "genes": [], "id": "IGIGPGHMMC_470", "locus": "GCA000148625_00437" }, { "type": "cds", "sequence": "contig_1", "start": 241396, "stop": 241644, "strand": "+", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "nt": "TTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCAAAACATAATTAA", "aa": "MKEKNAIQSMSRKGNCLDNSPTECLFSVIKREFWFGEEKKFNSFKEFKTALEEYISYYNNDRIVNKLKGLSPVQYRNKSKHN", "aa_hexdigest": "2886c266660afe5f4866afcaa139d6a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0003B3129C", "query_cov": 0.9878048780487805, "subject_cov": 1.0, "identity": 0.877, "score": 143.0, "evalue": 3.96e-43, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0003B3129C" }, "pscc": { "uniref50_id": "UniRef50_UPI0003B3129C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "product": "IS3 family transposase" }, "genes": [], "id": "IGIGPGHMMC_471", "locus": "GCA000148625_00438" }, { "type": "tRNA", "sequence": "contig_1", "start": 241781, "stop": 241854, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 79.5, "nt": "GCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 241813, 241815 ], "id": "IGIGPGHMMC_7", "locus": "GCA000148625_00439" }, { "type": "cds", "sequence": "contig_1", "start": 241954, "stop": 242703, "strand": "-", "frame": 2, "gene": null, "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9", "UniRef:UniRef90_C4XEK9" ], "nt": "ATGGGTGGAATTGAAAATATTACAGGTTTTAATAACTGTGCTGTTCGTCTTAGATATGATGTTAAAGATACTTCATTAGTAAATGAAGAAGCTCTTAAAAAAGTTGGGGCAAGTGAAGTTATTTTTATTGGAAAAAGACATGTGCAAGCTAAATTTGGTGAAATTAGTGAAAAATTAAATTTAGATATTCAAGAAGCTGCACCATTCTTAGAAGAAGAAGCACTTAAAAATAAAAAAATTGAAGTTAAAAGTGATGTCAACTTATTTGAAGAAAAACCGGTTGAAGTTAAAAAAACTGTTGATGAAAATATAGTTTATACACCTTGCAAAGGTACAAGAAAATCAATTGAAGATTTAAGTGATCAAACCTTTAGTTTGCTAGGTTGTGGTTATGCAATTGAAATTGATAAAAAATTAAACAAATTAGAAGTATATGCACCAATTAATGGAAAATTAATTATGTCATATCCAACTAAACATGCATATGGTATAGCAAATGATAATGGTCTTGAAGTTTTAGTACATATTGGTATTGATACAGTTAAATTAAATGGTTTAGGCTTTGAAACAAATCTTAAACAAAATGATAAAGTTGAAAGAGGACAACTCTTAGCAACTGTTGATCTTAAGAAATTAAGAAATAGTAAAATTTGTAGTGATGTTATTGTTTTAATTACAGATAATGTTAATAAAAACAAACAAACAAAATTGCTTTGTCCTTTAGAAATCAAAGAAGTAAATGTTCCTTGA", "aa": "MGGIENITGFNNCAVRLRYDVKDTSLVNEEALKKVGASEVIFIGKRHVQAKFGEISEKLNLDIQEAAPFLEEEALKNKKIEVKSDVNLFEEKPVEVKKTVDENIVYTPCKGTRKSIEDLSDQTFSLLGCGYAIEIDKKLNKLEVYAPINGKLIMSYPTKHAYGIANDNGLEVLVHIGIDTVKLNGLGFETNLKQNDKVERGQLLATVDLKKLRNSKICSDVIVLITDNVNKNKQTKLLCPLEIKEVNVP", "aa_hexdigest": "c2732983f364223a965ba0d7fe98c794", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEK9", "query_cov": 1.0, "subject_cov": 0.8469387755102041, "identity": 1.0, "score": 483.0, "evalue": 1.84e-171, "valid": true, "uniref50_id": "UniRef50_A0A449B3Q9" }, "pscc": { "uniref50_id": "UniRef50_A0A449B3Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9" ], "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component" }, "genes": [], "id": "IGIGPGHMMC_472", "locus": "GCA000148625_00440" }, { "type": "cds", "sequence": "contig_1", "start": 243062, "stop": 243274, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00389.36" ], "nt": "ATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGCCGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGA", "aa": "MKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKV", "aa_hexdigest": "24160e7e57cd710d2fea81ee33e9c68b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00389.36", "name": "2-Hacid_dh", "length": 63, "aa_cov": 0.8857142857142857, "hmm_cov": 0.9523809523809523, "evalue": 1.9920412764854152e-07, "score": 26.80187225341797, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 8092.2386, "isoelectric_point": 7.8017866134643565 }, "id": "IGIGPGHMMC_473", "locus": "GCA000148625_00441" }, { "type": "cds", "sequence": "contig_1", "start": 243287, "stop": 243925, "strand": "+", "frame": 2, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0", "UniRef:UniRef90_C4XEL0" ], "nt": "ATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGA", "aa": "MGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPE", "aa_hexdigest": "861682a6d127cd52b70f2926599b40dd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 243047, "stop": 244099, "observations": { "mutation": [ 243272, 243923 ], "stop": [ 243272, 243923 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL0", "query_cov": 1.0, "subject_cov": 0.6057142857142858, "identity": 1.0, "score": 411.0, "evalue": 6.34e-143, "gene_start": 1, "gene_end": 212, "reference_start": 81, "reference_end": 292, "reference_sequence": "MLGEKMKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKVWLQRSMGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPEWEELISNENVIITSHQAFLTDVALTQIAKITLENADMAQNKDFSKALVIMENGKVKNG" }, "paralog": false, "identity": 0.9914285714285714, "score": 677.0, "evalue": 1.72e-250, "description": "Internal stop codon at 243272, 243923. Nonsense mutation around 243272, 243923.", "psc": { "uniref90_id": "UniRef90_C4XEL0", "product": "D-lactate dehydrogenase", "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL0", "UniRef:UniRef50_C4XEL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0" ], "product": "D-lactate dehydrogenase" } }, "id": "IGIGPGHMMC_474", "locus": "GCA000148625_00442" }, { "type": "cds", "sequence": "contig_1", "start": 244500, "stop": 244736, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGA", "aa": "MAKLAMQIVGGILAGLIIYGIGHNASGIKFEANYLANATDTAHKANHYISAHNVSAKSFMPNFDSAFAKEAVITKKTI", "aa_hexdigest": "5de9688470557a7fcbe4adac6461d93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8279.486499999999, "isoelectric_point": 9.399442100524901 }, "id": "IGIGPGHMMC_475", "locus": "GCA000148625_00443" }, { "type": "cds", "sequence": "contig_1", "start": 244767, "stop": 244868, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGA", "aa": "MVLGAILLFPIFSPRLDNKYRDTFICFIISFDV", "aa_hexdigest": "2db4f4ce743334ed205d39dad0d1a35c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3865.603400000001, "isoelectric_point": 5.791972923278809 }, "id": "IGIGPGHMMC_476", "locus": "GCA000148625_00444" }, { "type": "cds", "sequence": "contig_1", "start": 244869, "stop": 244997, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": 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246566, "stop": 246847, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGA", "aa": "MLSKATKNFTNALRYPNQYLYGLYGVDSNKFKNRSEFLTEKDGREFINFTQLAGKEDITSFQDLLYNVATSKDFVAAPSQYIKETMNSPKISV", "aa_hexdigest": "eae2c119c5f30dca23c210a897b02a19", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10662.89459999999, "isoelectric_point": 8.859325981140138 }, "id": "IGIGPGHMMC_478", "locus": "GCA000148625_00446" }, { "type": "cds", "sequence": "contig_1", "start": 246965, "stop": 247498, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTCCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGA", "aa": "MLYAGRYFSAGYYPEASEYRFYKNRFYFDKDFVESEKNFGRVVTKYYSTTEDVFVAQIFDLYQKGKLHTLSFAQLNQQQQLDVTKNPQKYGLEYRQSLNKLNSPFKLLFNTIPYGKNFNADTKNRFLNVHAAKLFYGLTDNQVHNGTTQEETNEDLYVNMLSGRSVAFRSLLTAAVN", "aa_hexdigest": "10f3d075eefbfa456539ad7b720d677d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20701.935900000004, "isoelectric_point": 9.079421043395996 }, "id": "IGIGPGHMMC_479", "locus": "GCA000148625_00447" }, { "type": "cds", "sequence": "contig_1", "start": 247880, "stop": 247987, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGA", "aa": "MEKVFKSLESLIKSIDPRLKPSYKKFESSESDKAK", "aa_hexdigest": "c55eec0a479edb08ab97ae7dc1f732df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4074.6954000000023, "isoelectric_point": 9.456109809875489 }, "id": "IGIGPGHMMC_480", "locus": "GCA000148625_00448" }, { "type": "cds", "sequence": "contig_1", "start": 248351, "stop": 248557, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTGACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACATTAATTAATAGCGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGA", "aa": "MSSVISSQFFVSYVKKHTNAQVLTLTKEIMNYMGVVVDPAKTIANENFGPTLINSEYYEVPFAADNNL", "aa_hexdigest": "061079b51cb3d0d182a058b0fd5d5ede", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7591.557, "isoelectric_point": 5.004696083068848 }, "id": "IGIGPGHMMC_481", "locus": "GCA000148625_00449" }, { "type": "cds", "sequence": "contig_1", "start": 248620, "stop": 248928, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGA", "aa": "MVKYIFKRIAFAILTLFIIIIFSYSIIIAFQQNPYRIAALEEGLSKLEVQQKLQQAEEFDQTPGIVKLFNYLINIFQFNYGEVYVYKSEYETIPEMFFEPLQ", "aa_hexdigest": "3b6c2ef26f2827f064866588a9387984", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12151.050899999995, "isoelectric_point": 4.854925346374511 }, "id": "IGIGPGHMMC_482", "locus": "GCA000148625_00450" }, { "type": "cds", "sequence": "contig_1", "start": 248874, "stop": 249044, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTTTATCAACGTATCTCAAATTGTTCCTCTTTTATATCCAGCTAAAACGCCAATTGCCACACCTAAAATGGCACTAATAACGAAAGCTGGTAATGAAATTAAAATAGTTCATTGTAAGGGTTCAAAAAACATTTCTGGAATTGTTTCATATTCTGATTTATAA", "aa": "MKMFINVSQIVPLLYPAKTPIATPKMALITKAGNEIKIVHCKGSKNISGIVSYSDL", "aa_hexdigest": "5724190ec6b6b673e91cf2efc5536061", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6074.2899, "isoelectric_point": 9.732872581481931 }, "id": "IGIGPGHMMC_483", "locus": "GCA000148625_00451" }, { "type": "cds", "sequence": "contig_1", "start": 249199, "stop": 249420, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTTTATTAAGTGGAAGTATTATTATTGAAAGATTCTGA", "aa": "MTLGSLSVYTLYSRNQVITVLTSNYILIAKTKGLSGSKIFFKYVFRNISIPLAAIIIPSYIGLLSGSIIIERF", "aa_hexdigest": "7287c065c528190c491d732c78dba455", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.574099999996, "isoelectric_point": 10.08996295928955 }, "id": "IGIGPGHMMC_484", "locus": "GCA000148625_00452" }, { "type": "cds", "sequence": "contig_1", "start": 249421, "stop": 249600, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGA", "aa": "MVKGTSDLIVNAFPKGETNIVMFNILFFTTLSLFTEVLVDVTFTLIDPRIKYSKSGGIN", "aa_hexdigest": "b90260ee76ab9263feebfa26e750f73e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6557.626, "isoelectric_point": 6.000572013854979 }, "id": "IGIGPGHMMC_485", "locus": "GCA000148625_00453" }, { "type": "cds", "sequence": "contig_1", "start": 249700, "stop": 250047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12911.12" ], "nt": "ATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGA", "aa": "MDSRTFNDKYNISDELIQKIKLAEKEKKSTNIVGKPKILFYEICKRFFKNPVVLISFTAFIALLLTGIIVTLTSPYSPTGSILKGGDELTQNLPPVFSPVITNASNYETFQRMKE", "aa_hexdigest": "83bdb90f2be798f2de7b7e896720e0d8", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12911.12", "name": "OppC_N", "length": 59, "aa_cov": 0.4782608695652174, "hmm_cov": 0.847457627118644, "evalue": 1.1691731344135396e-08, "score": 30.81315803527832, "start": 41, "stop": 95 } ], "seq_stats": { "molecular_weight": 13009.031499999983, "isoelectric_point": 9.239431571960449 }, "id": "IGIGPGHMMC_486", "locus": "GCA000148625_00454" }, { "type": "cds", "sequence": "contig_1", "start": 250146, "stop": 250460, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGTAATAATCAAATAACTGAAGGCGGTGCTTGGAAGATTTCAATAACCCTCATCATTACAGTGTCAAGTCATTTTCCAGCATGGAAACCAAGAATAGCACCAACAGCAACACCAATTACAACCTCAATAGTTGCAACAATTAAAGCAACTTTAATTGAGCTAATTGCACCAAATCAAGCGCCTGTTCAAATATCTGCTTCTTTAACTGTTGTACCAAAAAGAGTATTTAAATGTGGCATTGAAGCTTTGATGTTTTCTATATCTTCTGGTCTTAAAGGATTAATTTTTAAAGCACGTGATCAAATATAA", "aa": "MNSNNQITEGGAWKISITLIITVSSHFPAWKPRIAPTATPITTSIVATIKATLIELIAPNQAPVQISASLTVVPKRVFKCGIEALMFSISSGLKGLIFKARDQI", "aa_hexdigest": "6491be8bf45c702e1f23eb3de7b5af6f", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11145.06689999999, "isoelectric_point": 10.132383155822755 }, "id": "IGIGPGHMMC_487", "locus": "GCA000148625_00455" }, { "type": "cds", "sequence": "contig_1", "start": 250543, "stop": 250800, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGA", "aa": "MFIITVKDEEYIIAAKSVGASTSRQIFFHALPAILGKIAMNYVRRIPSIILSIASLAFLGFYNKDTDINLGKFIYDNINDAKTNP", "aa_hexdigest": "b1dc4ed1cb0aa22119e4a5794d206c2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9483.959199999985, "isoelectric_point": 9.048863029479982 }, "id": "IGIGPGHMMC_488", "locus": "GCA000148625_00456" }, { "type": "cds", "sequence": "contig_1", "start": 250916, "stop": 251239, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "nt": "ATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAACTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATACTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGA", "aa": "MTSVKEKKNIKKRNEDIKAEKLANDLLLKVRNLRVDFKVGRNKYLRIVRGIDLDIFKGQIVGLVGESGSGKSVTSKSLINVNEGATTTADFMQIEDINLNKFKKERQ", "aa_hexdigest": "f9cf7bbf4a1e88555798cfcb2bb8ff90", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FDFF16B", "query_cov": 0.8411214953271028, "subject_cov": 0.8108108108108109, "identity": 0.611, "score": 101.0, "evalue": 7.27e-26, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002FDFF16B" }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFF16B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_489", "locus": "GCA000148625_00457" }, { "type": "cds", "sequence": "contig_1", "start": 251390, "stop": 251752, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9", "UniRef:UniRef90_UPI002FE1EF1F" ], "nt": "TTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCATCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGA", "aa": "MVGLLDKFGIRDSEQVFDRYPHTLSGGMKQRVVITMVVALKPSLIIADEPTTALDPTVQASVLALFEKIHQEMNISIILISHNISVVAKFCDYIYVMYAGRIVEKGTRKEIFTDPCHPYT", "aa_hexdigest": "ecbdf3121e12b634aef6e3a9148d895e", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0022487FDE", "query_cov": 0.9916666666666667, "subject_cov": 0.8623188405797102, "identity": 0.504, "score": 117.0, "evalue": 1.09e-31, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A838SM00" }, "genes": [], "truncated": "both", "pseudogene": { "start": 251312, "stop": 252037, "observations": { "mutation": [ 251354, 251750, 251939, 252029 ], "stop": [ 251354, 251750, 251939, 252029 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "query_cov": 1.0, "subject_cov": 0.4979253112033195, "identity": 0.8, "score": 195.0, "evalue": 5.41e-61, "gene_start": 1, "gene_end": 120, "reference_start": 27, "reference_end": 146, "reference_sequence": "MGKQLLDALNNNEDWKDKTYSEKKNYLLDLLKTFGLRNVEKIFMSYPHTLSGGMKQRIVITMVVALKPDVIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVERGTKQEIFTDPAHPYTWALISAIPESKEEKLYNIKGTPPDMANLPIGDPFAVRSDYALEIDFIKEPPLIPITQTHAAATWLLSPEAPKIEKSDDLKKRLELFKKAFNHEQK" }, "paralog": false, "identity": 0.7178423236514523, "score": 353.0, "evalue": 9.53e-126, "description": "Internal stop codon at 251354, 251750, 251939, 252029. Nonsense mutation around 251354, 251750, 251939, 252029.", "psc": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE1EF1F", "UniRef:UniRef50_A0A223MAZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "IGIGPGHMMC_490", "locus": "GCA000148625_00458" }, { "type": "cds", "sequence": "contig_1", "start": 251822, "stop": 251941, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGA", "aa": "MANLPLGDPFAPRNDYALEIDFYKEPPLIEVSETHAAAT", "aa_hexdigest": "67c325ea9b5612a2a08640ce93aaf67e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4317.782400000001, "isoelectric_point": 4.192523765563965 }, "id": "IGIGPGHMMC_491", "locus": "GCA000148625_00459" }, { "type": "cds", "sequence": "contig_1", "start": 252024, "stop": 252422, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGA", "aa": "MNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKD", "aa_hexdigest": "2a23d78d0acce62e0e3ffc4d1872fe20", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9242424242424242, "subject_cov": 0.953125, "identity": 0.779, "score": 193.0, "evalue": 1.38e-61, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_492", "locus": "GCA000148625_00460" }, { "type": "cds", "sequence": "contig_1", "start": 252552, "stop": 254375, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6", "UniRef:UniRef90_C4XEL6" ], "nt": "ATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCATAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGA", "aa": "MNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSIIDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEE", "aa_hexdigest": "1b9cf14da128e063efd0ff28d277cd44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 252252, "stop": 254675, "observations": { "mutation": [ 252420, 252531, 254373 ], "stop": [ 252420, 252531, 254373 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL6", "query_cov": 1.0, "subject_cov": 0.6827896512935883, "identity": 0.997, "score": 990.0, "evalue": 0.0, "gene_start": 1, "gene_end": 607, "reference_start": 183, "reference_end": 789, "reference_sequence": "MERYLKMNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKDWFDIKENFFYVFKQRALELKLKNLESINLLAEKFFPYWIEKFKKMNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSITDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEEWVKPFNINSFSLATNKSIDEEYNSKIDHKPYDGHSEIPNNIEYTDEIKIDEPTCEIKISSNFDFSKPIVEVVETKVSTTKTTKKRSTSRKASSAKKTTKK" }, "paralog": false, "identity": 0.9938042131350682, "score": 1395.0, "evalue": 0.0, "description": "Internal stop codon at 252420, 252531, 254373. Nonsense mutation around 252420, 252531, 254373.", "psc": { "uniref90_id": "UniRef90_C4XEL6", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL6", "UniRef:UniRef50_C4XEL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_493", "locus": "GCA000148625_00461" }, { "type": "cds", "sequence": "contig_1", "start": 254803, "stop": 255174, "strand": "+", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7", "UniRef:UniRef90_C4XEL7" ], "nt": "TTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAA", "aa": "MYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM", "aa_hexdigest": "4f93706ad97d0fb4bb6cc06fbf692075", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 254692, "stop": 255174, "observations": { "mutation": [ 254737 ], "stop": [ 254737 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL7", "query_cov": 1.0, "subject_cov": 0.76875, "identity": 0.992, "score": 214.0, "evalue": 1.62e-69, "gene_start": 1, "gene_end": 123, "reference_start": 38, "reference_end": 160, "reference_sequence": "MTMNSTREYFREAFTWKKLLHLFIILLISLIAGVSLYLYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM" }, "paralog": false, "identity": 0.9875, "score": 298.0, "evalue": 6.77e-105, "description": "Internal stop codon at 254737. Nonsense mutation around 254737.", "psc": { "uniref90_id": "UniRef90_C4XEL7", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL7", "UniRef:UniRef50_C4XEL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7" ], "product": "DUF3899 domain-containing protein" } }, "id": "IGIGPGHMMC_494", "locus": "GCA000148625_00462" }, { "type": "cds", "sequence": "contig_1", "start": 255266, "stop": 256351, "strand": "+", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "nt": "ATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGAAGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGA", "aa": "MSNLYQTKRDDNILLKAYYEANKNKEIKIDQLNYKTGKITEFLNKKEAYIFLDKNFKNYDQLVDLIDTKIVTKNRNYQIDLESFVNKDFPIESVLKAFYSRIIFYTYNYFNKKKEAPKKNQYTFLLKDNSHKELANKFELIANNRNLVRNLQVMPENFCNSEQLAQYIVNDFKNEKDLKVTVLNKKEIEKLNMGLIISVNKGSTHEPRVVIVEYNGDPNSKEKIVYVGKGITFDTGGMNTKGYHMEGMKYDMSGSVIVAYTVKTLAQLKAKKNVAAIMCITDNRQDGDASLPENVYESMSGITVEVTDTDAEGRLVLADGLYYGAKKLNATTLVDVATLTGAVLRALGTVYSGIYSTDEKN", "aa_hexdigest": "169972629da878c86c80e4f8864036e4", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00068F9161", "query_cov": 1.005540166204986, "subject_cov": 0.9784366576819407, "identity": 0.6940000000000001, "score": 482.0, "evalue": 4.91e-168, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_A0A449ADP4" }, "pscc": { "uniref50_id": "UniRef50_A0A449ADP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "product": "Cytosol aminopeptidase" }, "genes": [], "id": "IGIGPGHMMC_495", "locus": "GCA000148625_00463" }, { "type": "cds", "sequence": "contig_1", "start": 256400, "stop": 256630, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAA", "aa": "MPLHEDFHEPNTASIVADLNNYNNDEKSDCNTAAMFLKQFTNDVPYIHCDVAGTADLSGKPQGVLVDTLVEFALLK", "aa_hexdigest": "2d8095404762afb20e369da7dd37587b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.9078947368421053, "hmm_cov": 0.974025974025974, "evalue": 3.680884346949086e-14, "score": 48.7059440612793, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 8368.247, "isoelectric_point": 4.345022773742676 }, "id": "IGIGPGHMMC_496", "locus": "GCA000148625_00464" }, { "type": "cds", "sequence": "contig_1", "start": 256842, "stop": 257333, "strand": "+", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "nt": "ATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACCGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGA", "aa": "MEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQL", "aa_hexdigest": "cd0eed35ea2c69e80d192ebeb0188957", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q6MUD9", "query_cov": 1.0552147239263803, "subject_cov": 0.8349514563106796, "identity": 0.512, "score": 173.0, "evalue": 4.4e-52, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_Q6MUD9", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_Q6MUD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_497", "locus": "GCA000148625_00465" }, { "type": "cds", "sequence": "contig_1", "start": 257358, "stop": 258092, "strand": "+", "frame": 3, "gene": null, "product": "ATPase", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEL9", "UniRef:UniRef90_C4XEL9" ], "nt": "ATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGACTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGCTGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGGCTTTTTATTAAATGATAACATTTTTTAA", "aa": "MPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLDYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQSCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFGFLLNDNIF", "aa_hexdigest": "e04d8b2844997834992a1db54229929d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 257058, "stop": 258092, "observations": { "mutation": [ 257331 ], "stop": [ 257331 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL9", "query_cov": 1.0, "subject_cov": 0.5795724465558195, "identity": 0.988, "score": 463.0, "evalue": 5.6e-162, "gene_start": 1, "gene_end": 244, "reference_start": 178, "reference_end": 421, "reference_sequence": "MHEVFMEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQLWKEYCEFGGMPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLVYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQRCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFDFLLNDNIF" }, "paralog": false, "identity": 0.9883720930232558, "score": 674.0, "evalue": 2.84e-247, "description": "Internal stop codon at 257331. Nonsense mutation around 257331.", "psc": { "uniref90_id": "UniRef90_C4XEL9", "product": "ATPase", "uniref50_id": "UniRef50_C4XEL9", "kegg_orthology_id": "K07133", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL9", "UniRef:UniRef50_C4XEL9", "KEGG:K07133" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL9" ], "product": "ATPase" } }, "id": "IGIGPGHMMC_498", "locus": "GCA000148625_00466" }, { "type": "cds", "sequence": "contig_1", "start": 259214, "stop": 259378, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTGAACAAGGTGATTATGCTGCTATTGAAATCAAACTTGGCTCAATGCAAGAAGCTGGTGCTATAGAAAACTTAAGAAAATTTAGAGATAGTATGATTAAAGAAGCTTCTTTAGCTAAAAACGAAAGTGAAAAACAATATAAAAAAGTTCCTAAATGA", "aa": "MKTEQGDYAAIEIKLGSMQEAGAIENLRKFRDSMIKEASLAKNESEKQYKKVPK", "aa_hexdigest": "f6417c4fdaebfb0477ecf45c42866c93", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6132.995000000001, "isoelectric_point": 9.003799629211429 }, "id": "IGIGPGHMMC_499", "locus": "GCA000148625_00467" }, { "type": "cds", "sequence": "contig_1", "start": 259400, "stop": 259780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "ATGTTTCTAAAATCTTTGGCTCGTAATGAGGGCCAAACTTGCAAAAAAACAACTATTGCTAAAGACATTATGGAATATGAAAATGAAAATATCAACATTAAAACAATTAATAATTATTTTAAAACTGCAGAAAGTTTATATTTAATAGAAAATCAAACCTACTTTAAACCTAACTTTTTAAGTAGTAATTATGTTTTAAATTCTGAAAAAATAAGATTTATTGATCCTTCAATTCCTGCAGGAATATTAAAATTAAGTCCTCAACTATTAAATGAAACAAAAAATACACTAGGTGTTTATTTTGAATCTTTAGTAATAAGAGACTTAAGAATTTATAGTCAATTAATTGATGTCGAAGTCCTTTTTTATCGTAACAAATGA", "aa": "MFLKSLARNEGQTCKKTTIAKDIMEYENENINIKTINNYFKTAESLYLIENQTYFKPNFLSSNYVLNSEKIRFIDPSIPAGILKLSPQLLNETKNTLGVYFESLVIRDLRIYSQLIDVEVLFYRNK", "aa_hexdigest": "c7f201f3e3e77d06afd077688bf061d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 126, "aa_cov": 0.9920634920634921, "hmm_cov": 0.9365079365079365, "evalue": 3.0762296352097354e-18, "score": 62.05604553222656, "start": 2, "stop": 126 } ], "seq_stats": { "molecular_weight": 14757.837399999984, "isoelectric_point": 8.605191612243654 }, "id": "IGIGPGHMMC_500", "locus": "GCA000148625_00468" }, { "type": "cds", "sequence": "contig_1", "start": 259904, "stop": 260170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTACCAAAATTAATGAAACAGGTAAACCAGGTCAATTTATTTTAACTGGTTCTTTTCAACCTGTTGTCAAAAAACCTAAACACTCAGGTTCTGGAAGAATTGGTAGATTAAAAATGTATCCAATGAGTTTAAGTGAAATGAATATTTCTTCAAAACAAATTTCATTAAGTAAATTAATCAATGAAAATAAATTTGAACAAGTTAAAGTTAAAAAACCTTTTGATACTTTTGAATTAGCTCAAATTATAATCAAAGGCGGATGA", "aa": "MITKINETGKPGQFILTGSFQPVVKKPKHSGSGRIGRLKMYPMSLSEMNISSKQISLSKLINENKFEQVKVKKPFDTFELAQIIIKGG", "aa_hexdigest": "1d2f08e3f2e06eb41c42a3e700b276e2", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9823.527099999992, "isoelectric_point": 10.121745872497556 }, "id": "IGIGPGHMMC_501", "locus": "GCA000148625_00469" }, { "type": "cds", "sequence": "contig_1", "start": 260324, "stop": 260452, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCAAAAATTAATTATGATAAAAATAATTATCAAACAAGAATAGTTGACTCTTTACTTGAAAAACAATTAAAAGTTATGGGAGCTGTTTTAGTTTATGGTCCTAGATATTGTGGCAAAACATGA", "aa": "MTAKINYDKNNYQTRIVDSLLEKQLKVMGAVLVYGPRYCGKT", "aa_hexdigest": "7746af4701a4db7cd71d75dd6170b8e0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4823.6144, "isoelectric_point": 9.517548179626463 }, "id": "IGIGPGHMMC_502", "locus": "GCA000148625_00470" }, { "type": "cds", "sequence": "contig_1", "start": 260823, "stop": 261302, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0", "UniRef:UniRef90_C4XEM0" ], "nt": "ATGGCTAAAGCAGTTTATTTTTCAGATTTAAATTTAACTAAAGTAAAATTTCCAAACAATATTAATAAAGATTTAATTGCGAGAGTTGCACATAAATTAGACGATATTTGTGAAGAAATGTGCAATCAAAAAAGCGAAATTAGTTTTTATGTTGAAAAAACTTTTCCATTTGGTCAAAAAATGTTAGCTATTGTTGATGACACACTTGCATATGACTTCAACATTATTTTAAAACCTAAAAAAACTATTAATTTAGAAAGTGTTGATTTCAACAAATATGTACTTGAAAAGCTACAAAAACATATTTGTCCATTAGGTTTTAGTACAAAAATTGTTGAAAATTCAAAAAGTTTCAATTTAAAATTTACTGATTTCAAAAATTTTTCAATTGATATTGCTTTATACAGCGAAAATAAAAATAATGAAAGATTTGTTCTAGTTAGATCTAAAGATAAAGCTAATAAAATAGTTTACAATTGA", "aa": "MAKAVYFSDLNLTKVKFPNNINKDLIARVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESVDFNKYVLEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYN", "aa_hexdigest": "f0563182be5344f40626f803f1c0e929", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 260622, "stop": 261338, "observations": { "mutation": [ 260756, 260825 ], "stop": [ 260756, 260825 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM0", "query_cov": 1.0, "subject_cov": 0.6680672268907563, "identity": 0.975, "score": 311.0, "evalue": 4.04e-106, "gene_start": 1, "gene_end": 159, "reference_start": 13, "reference_end": 171, "reference_sequence": "MILWKKLEKRKIMAKAVYFSDLNLIKVKFPNNINKDLITRVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESADFNKYILEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYNWTKVYDYEYTGDKVAEIKKYDRIWKETKELFLKKSEKNPDIDNFSHKTAFNLAVDAVYSVFKKYNNF" }, "paralog": false, "identity": 0.957983193277311, "score": 456.0, "evalue": 1.29e-165, "description": "Internal stop codon at 260756, 260825. Nonsense mutation around 260756, 260825.", "psc": { "uniref90_id": "UniRef90_C4XEM0", "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM0", "UniRef:UniRef50_C4XEM0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18508.252699999997, "isoelectric_point": 8.994838523864747 }, "id": "IGIGPGHMMC_503", "locus": "GCA000148625_00471" }, { "type": "cds", "sequence": "contig_1", "start": 261805, "stop": 262248, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGCCTACTTCAAAAGTAAAAATATCAAACAATGAAAATCAAAATTGCTTGCAAATTGGTGACGTAATTTTCTCGGGTTCTTCAGAAAATTTTGAAGAAACAGGTTATGCTTCAGTTTTTAATTTAATTTCAGAAAACAAAATTTATTTAAATAGTTTTTGCTTTGCAATAAGATTTAACAACAAAAATTTATTTTTGCCAAAGTTTTCAAAATACTTGTTTAATTCTGAAATATTTAGAAATCAATTAGTAAAGTGTATTAATGGAGTTACAAGATTTAACTTATCAAAAGTAAAATTTGCCTCAATTAAAGTTCCTTTAATTCCTCTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATCCTAGAAGCGGAGCTGAAAGCGGAGCTGAAAGCGGAGTTGGAAGCGAGGGGGAAGCAATTTAATTTTTGA", "aa": "MPTSKVKISNNENQNCLQIGDVIFSGSSENFEETGYASVFNLISENKIYLNSFCFAIRFNNKNLFLPKFSKYLFNSEIFRNQLVKCINGVTRFNLSKVKFASIKVPLIPLKIQEKIVEILERFRILEAELKAELKAELEARGKQFNF", "aa_hexdigest": "e19e7ea4423bff15b82295ad4e2ef1fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 133, "aa_cov": 0.9047619047619048, "hmm_cov": 0.9097744360902256, "evalue": 1.3991264924621278e-13, "score": 47.41664123535156, "start": 7, "stop": 139 } ], "seq_stats": { "molecular_weight": 16998.55659999999, "isoelectric_point": 9.300096321105958 }, "id": "IGIGPGHMMC_504", "locus": "GCA000148625_00472" }, { "type": "cds", "sequence": "contig_1", "start": 262294, "stop": 262812, "strand": "+", "frame": 1, "gene": null, "product": "DNA specificity protein of restriction modification system", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377HVD6" ], "nt": "TTGGAAAACCTAGATATTAATATTGAAACTGGAAAATTAAATGCAAATAAAAAAAATGAAAATGGAAAGTATTTATTTTTTACATGTGATGAAAAACCTTATCGTATTAATGAATATGCATTTGATGCAGAATCAATATTAATATCTGGTAATGGTTCTAAATTAGGACATATTAGTTATTATGAAGGAAAATTTAATGCTTATCAAAGGACTTATGTTTTAACTTCTAAAGATGTAAATATAAACCTAAAGTATTTATATTATTTCTTAAAACATAATTTTAAAGATTACATTTCTTCAATTCATTTTGGATCTTCATCTGTTCCATATATTACACTTCCTATATTACAAGAATATAAATTAAAATTACCCCCTCTTGAAATCCAAAACAAGATAGTTTCAATTCTTGACAAATTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAA", "aa": "MENLDINIETGKLNANKKNENGKYLFFTCDEKPYRINEYAFDAESILISGNGSKLGHISYYEGKFNAYQRTYVLTSKDVNINLKYLYYFLKHNFKDYISSIHFGSSSVPYITLPILQEYKLKLPPLEIQNKIVSILDKLSEYSQEINSGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "242d69627a372ed6763235be5443a862", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034E4CEE4", "query_cov": 0.9941860465116279, "subject_cov": 0.8300970873786407, "identity": 0.532, "score": 177.0, "evalue": 1.88e-53, "valid": false, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A377HVD6", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0A377HVD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377HVD6" ], "product": "DNA specificity protein of restriction modification system" }, "genes": [], "id": "IGIGPGHMMC_505", "locus": "GCA000148625_00473" }, { "type": "cds", "sequence": "contig_1", "start": 263061, "stop": 264035, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2", "UniRef:UniRef90_C4XEM2", "PFAM:PF04200.17", "PFAM:PF13306.12" ], "nt": "ATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAACTTAACCTCACTCGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAGGCTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGA", "aa": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTGLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVS", "aa_hexdigest": "fda7eb5f61dc6e573c2e5ce9db2c74d5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 263061, "stop": 264302, "observations": { "mutation": [ 264033 ], "stop": [ 264033 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM2", "query_cov": 1.0, "subject_cov": 0.542713567839196, "identity": 0.997, "score": 462.0, "evalue": 1.33e-157, "gene_start": 1, "gene_end": 324, "reference_start": 1, "reference_end": 324, "reference_sequence": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTSLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVSWAAFKGCTSLTEINLPENVKFIGKHAFLNCSNLAKVEMKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTTITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTGWDNKDIKSKAFYGCDVLTTVTFASKVKNILKEAFFACPLLKEVSIHKGTDWKTGLMNKNKAFDETITTVKVIN" }, "paralog": false, "identity": 0.9927360774818402, "score": 491.0, "evalue": 1.58e-171, "description": "Internal stop codon at 264033. Nonsense mutation around 264033.", "psc": { "uniref90_id": "UniRef90_C4XEM2", "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM2", "UniRef:UniRef50_C4XEM2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 81, "aa_cov": 0.22530864197530864, "hmm_cov": 0.9259259259259259, "evalue": 1.6799322824780658e-06, "score": 24.336694717407227, "start": 50, "stop": 122 }, { "id": "PF13306.12", "name": "LRR_5", "length": 86, "aa_cov": 0.2654320987654321, "hmm_cov": 0.9883720930232558, "evalue": 8.442599094047296e-35, "score": 115.48124694824219, "start": 189, "stop": 274 } ], "seq_stats": { "molecular_weight": 34686.49300000004, "isoelectric_point": 8.186598396301271 }, "id": "IGIGPGHMMC_506", "locus": "GCA000148625_00474" }, { "type": "cds", "sequence": "contig_1", "start": 264144, "stop": 264635, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "ATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAACAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGA", "aa": "MKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTTITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTG", "aa_hexdigest": "ee906a2dee62d00aa63961c2a8eb06c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 105, "aa_cov": 0.6441717791411042, "hmm_cov": 0.9904761904761905, "evalue": 5.635792625335252e-48, "score": 158.06832885742188, "start": 2, "stop": 106 }, { "id": "PF13855.12", "name": "LRR_8", "length": 46, "aa_cov": 0.27607361963190186, "hmm_cov": 1.0, "evalue": 8.995637397449253e-16, "score": 53.48106384277344, "start": 49, "stop": 93 } ], "seq_stats": { "molecular_weight": 17336.678299999985, "isoelectric_point": 8.446212577819825 }, "id": "IGIGPGHMMC_507", "locus": "GCA000148625_00475" }, { "type": "cds", "sequence": "contig_1", "start": 264992, "stop": 266242, "strand": "+", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "EC:5.4.2.12", "GO:0006096", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_C4XEM3" ], "nt": "ATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGA", "aa": "MKRTILVVIDGLGLREEKQGNGFKLAKTPTFDKLFKEYPNSILQASGEYVGLPKGQMGNSEVGHLNIGAGTVVYTGLSLINKAMKDGEFEKNKTFNEVFADVKKKNSTLHLMGLLSPGGVHSLEDHLFKLIDMAHAYGLKKVSVHAFGDGRDVAPKSIITSLRKLQTQVDKYGYKIASIGGRFYGMDRDKMFDRVEKHYEALLGNSDHTYESVIDYVNSQYDEDITDEFFIPAIDKGANFVKDNDSIIFFNFRPDRARQLTHLFIGSKLYDNKPKHPVKISKFCSMMKYEGINTLVAFSEMEVKMPIGRVLEKAGKSQLRVAETQKYAHVTYFMDGGNDIEFKNSKRIMVPSLKVESYANAPHMSAEGITDELTKNVLDYDVTIMNYANPDMVGHTGNLKSTIEAVSFLDTQIKRL", "aa_hexdigest": "44271c866e7b4d78b026fe7ca9c32834", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEM3", "query_cov": 1.0, "subject_cov": 0.8205128205128205, "identity": 1.0, "score": 832.0, "evalue": 2.68e-303, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0006096", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "IGIGPGHMMC_508", "locus": "GCA000148625_00476" }, { "type": "cds", "sequence": "contig_1", "start": 266357, "stop": 266491, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAA", "aa": "MFICTDKNIKLANGKLANVAPTILDYIGVKQPKEMDEKSLLIRK", "aa_hexdigest": "ea90937aacb199a5afd32d3c427db213", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.905999999999, "isoelectric_point": 9.30203037261963 }, "id": "IGIGPGHMMC_509", "locus": "GCA000148625_00477" }, { "type": "cds", "sequence": "contig_1", "start": 266624, "stop": 267055, "strand": "+", "frame": 2, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9", "UniRef:UniRef90_C4XEM4" ], "nt": "ATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGA", "aa": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYD", "aa_hexdigest": "f630fc66a06dddf3e2a4bf7ded7145ae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 266624, "stop": 267358, "observations": { "mutation": [ 267053, 267233 ], "stop": [ 267053, 267233 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM4", "query_cov": 1.0, "subject_cov": 0.4847457627118644, "identity": 1.0, "score": 291.0, "evalue": 1.67e-97, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYDWNKSGEINQDLHKIELFGTSDEIKKAYEELKKLKLKLKVIYFGTHIEITPKGVDKGYGLKWICENIFNCNFDYVMSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY" }, "paralog": false, "identity": 0.9918032786885246, "score": 493.0, "evalue": 1.91e-179, "description": "Internal stop codon at 267053, 267233. Nonsense mutation around 267053, 267233.", "psc": { "uniref90_id": "UniRef90_C4XEM4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM4", "UniRef:UniRef50_A0A3P8L7G9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9" ], "product": "Cof-like hydrolase" } }, "id": "IGIGPGHMMC_510", "locus": "GCA000148625_00478" }, { "type": "cds", "sequence": "contig_1", "start": 267275, "stop": 267511, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAG", "aa": "MSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY", "aa_hexdigest": "8c425ea810c83680e259a29abca0aa1d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 49, "aa_cov": 0.6282051282051282, "hmm_cov": 1.0, "evalue": 3.088152063267398e-17, "score": 59.14257049560547, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8942.941199999994, "isoelectric_point": 5.769976234436036 }, "id": "IGIGPGHMMC_511", "locus": "GCA000148625_00479" }, { "type": "cds", "sequence": "contig_1", "start": 267805, "stop": 267966, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGA", "aa": "MALMLVMIILGSYFIAQPDNGSGKNVETYICLIFFGVYGFIITLLLMIYFLIL", "aa_hexdigest": "683c983f6bacf6df334e5565c1924d1f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5998.331799999996, "isoelectric_point": 4.3690088272094725 }, "id": "IGIGPGHMMC_512", "locus": "GCA000148625_00480" }, { "type": "cds", "sequence": "contig_1", "start": 268264, "stop": 268362, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVQQRP", "aa_hexdigest": "4cad5b797311cc167a306173bb16a06e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3644.063, "isoelectric_point": 10.834379386901855 }, "id": "IGIGPGHMMC_513", "locus": "GCA000148625_00481" }, { "type": "cds", "sequence": "contig_1", "start": 268363, "stop": 268776, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNAQPQNPQPNPYGQPNPQDSGMFQDPNGQDFGGFDPQGN", "aa_hexdigest": "da8c5cdf6bab30041c78fac129313762", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14979.199099999987, "isoelectric_point": 5.853131675720214 }, "id": "IGIGPGHMMC_514", "locus": "GCA000148625_00482" }, { "type": "cds", "sequence": "contig_1", "start": 268940, "stop": 270181, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q4AAP5", "UniRef:UniRef90_C4XEM6" ], "nt": "ATGGGTTTTAAATTTAAATCAACAGAAATGCGCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGTTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGA", "aa": "MGFKFKSTEMRIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKVQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAK", "aa_hexdigest": "e77ea70855ac1246e7b32bcc90dd0d22", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9782082324455206, "subject_cov": 0.9688249400479616, "identity": 0.557, "score": 438.0, "evalue": 3.51e-149, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 268937, "stop": 270484, "observations": { "mutation": [ 270179 ], "stop": [ 270179 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM6", "query_cov": 1.0, "subject_cov": 0.49108204518430437, "identity": 0.995, "score": 798.0, "evalue": 6.62e-285, "gene_start": 1, "gene_end": 413, "reference_start": 2, "reference_end": 414, "reference_sequence": "MMGFKFKSTEMLIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKAQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAKWKAVTEKIKELYEIGQPVLVGTAQIEDSEILHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKKWREQAIRNSNDDTDYINWYEKDLVLEIVDIYWQNHIDTMDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN" }, "paralog": false, "identity": 0.9922330097087378, "score": 998.0, "evalue": 0.0, "description": "Internal stop codon at 270179. Nonsense mutation around 270179.", "psc": { "uniref90_id": "UniRef90_C4XEM6", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q4AAP5", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM6", "EC:7.4.2.8", "UniRef:UniRef50_Q4AAP5", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q4AAP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4AAP5" ], "product": "Protein translocase subunit SecA" } }, "id": "IGIGPGHMMC_515", "locus": "GCA000148625_00483" }, { "type": "cds", "sequence": "contig_1", "start": 270269, "stop": 271033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "TTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGA", "aa": "MHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKK", "aa_hexdigest": "9aa2252219956fa6b5019b8302c8044b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 54, "aa_cov": 0.2125984251968504, "hmm_cov": 1.0, "evalue": 4.1814975679508627e-48, "score": 159.6897430419922, "start": 54, "stop": 107 } ], "seq_stats": { "molecular_weight": 29412.01260000001, "isoelectric_point": 7.811456871032715 }, "id": "IGIGPGHMMC_516", "locus": "GCA000148625_00484" }, { "type": "cds", "sequence": "contig_1", "start": 271145, "stop": 271462, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAA", "aa": "MDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN", "aa_hexdigest": "966fb897797673cecf218e001389645b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12499.864199999993, "isoelectric_point": 4.600741386413574 }, "id": "IGIGPGHMMC_517", "locus": "GCA000148625_00485" }, { "type": "cds", "sequence": "contig_1", "start": 271630, "stop": 271755, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGA", "aa": "MNTPQDNKNHEDKRVQVEIKRSKVKQSFLHFGSLYRVKKLR", "aa_hexdigest": "3f65eaa5943d982a099af793f7aab63e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4983.7145, "isoelectric_point": 10.446279716491699 }, "id": "IGIGPGHMMC_518", "locus": "GCA000148625_00486" }, { "type": "cds", "sequence": "contig_1", "start": 272434, "stop": 272832, "strand": "+", "frame": 1, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE", "UniRef:UniRef90_UPI000FF5F3CE" ], "nt": "ATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAG", "aa": "MVLINGVVLNVLFPKYKIVKVEIYSSKIPEIQEKINTLKDVRFATSHLSVKGGYSGQEQEVLVTMCLYLDAAHLLEIVREFDENALFTVTDLKKVDGYFYVSVENHKSLFNKKIENKEQKKTKENVEDNSKK", "aa_hexdigest": "d0b89e52f80b83486cc237f32fe05585", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF5F3CE", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.992, "score": 246.0, "evalue": 2.92e-82, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI000FF5F3CE" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5F3CE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_519", "locus": "GCA000148625_00487" }, { "type": "cds", "sequence": "contig_1", "start": 272894, "stop": 273157, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGA", "aa": "MENKNNQNELTNQNEIEIQRSKIKQSFLHFGTLYRVRSFKKQIIYTIIIGVLFGIVQHLLISITGLYNMGLDGLCQSIARIVRHFLR", "aa_hexdigest": "a108be0960c4f784d15c099e102f04d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10177.83499999999, "isoelectric_point": 10.006669807434083 }, "id": "IGIGPGHMMC_520", "locus": "GCA000148625_00488" }, { "type": "cds", "sequence": "contig_1", "start": 273605, "stop": 274129, "strand": "+", "frame": 2, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD", "UniRef:UniRef90_UPI00217FD9AD" ], "nt": "TTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAA", "aa": "MLFLFACNLIGSYIPFAVEDPIAASFSKHPGNRYELENFFNPSFVAGFIMVVVNGIVLNVLFPKFKMVKVEIISNQVEMIQQKLNTLKDRRYATSTLTVIGGYSHKEQKILLTTCLYIDAAKLLETVRGIDQNALFTVMDLKKADGYIYTTHEHQNHLFKHKKRDEINDQQKQN", "aa_hexdigest": "a5363b2b2ebc89e51b58f1a7dc4717ad", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00217FD9AD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 337.0, "evalue": 7.01e-117, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI00217FD9AD" }, "pscc": { "uniref50_id": "UniRef50_UPI00217FD9AD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_521", "locus": "GCA000148625_00489" }, { "type": "cds", "sequence": "contig_1", "start": 274550, "stop": 274876, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACAAAAACATGAGAATTGATATTAATCCAAAAAATTATACTTTTACTTTAAAAAAATTAGGTAATAATAAAAAGGAAAAACCAATTAATCTAACTTCGCTTAAATTAACAAAAGAAGCAATTGAAGCTTACAAAAAAACTGGATTGAAAAAACCATCTCAAGTTATAACACATCCTTCAAAACAAATTACAGGTAAAAATGCAGGTTCTCATGGATTTTTATATACAGTTCTTGATAAAAATGGCAAAGTCAACATTATATTTTCAATTAAAGAAAATAATGTAAAATCTAAAGACTTCTTAGCTAAAATTGAAGACTAG", "aa": "MNNKNMRIDINPKNYTFTLKKLGNNKKEKPINLTSLKLTKEAIEAYKKTGLKKPSQVITHPSKQITGKNAGSHGFLYTVLDKNGKVNIIFSIKENNVKSKDFLAKIED", "aa_hexdigest": "81037d5d29170d4cc97526bc406084bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12219.110099999985, "isoelectric_point": 9.999384880065918 }, "id": "IGIGPGHMMC_522", "locus": "GCA000148625_00490" }, { "type": "cds", "sequence": "contig_1", "start": 275111, "stop": 275887, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAACAAATGTTGACTTAAATGAAAAAGCAAGTAAAACAATTGAAGAATATATTGCAGCTCACACAAGTGATTTATTGGCTCAAGTTACAGCCACAACTCCAAGTGGTAAAACCTTAAGCGAATATCTAAGTGAAGAAAAGATTACAATTAATAAAACAGAATTAATTGTTGCTAATAATAATTTAGGTCAAGCAAAATTGAAATTAACTTTAAGTTTTGAAGATAAAAGTCAAGAAAATGTTGAAGTTGAATATGAAATTAATGATTTCAAAAAACCAGCTCCATATTCAGAAGACAAATTTGAAAACTTAATCGGTACTTTAAAAACTAAAATTAGTGAAAAGAACTATTATTCAGAAATTAAAGAAGAACAAAATTTAGTAAAACTTTACTACACTGATCCAGATACAAATGAAGAAAAAGAATTCAATAAAGAAAATCTTGAAAAATTGAATATTCAAGCCACAACAATTAATTTAAATAATTTAATGACATTAAATTTTGATGATGCAACACGTAAATTAACTATTACGGTTTCATACACAACTAAATCAACTCCAAAAGAAAAGAAATTTGAATTAACTTTTGCAGAAAAATCATTTGTAGAATTAAAAGATACATTTTTAGCTGAAATTAATCTTTCATTACCTAACCCTTGTGATGTTACAAAGGCTACAGATAATGACATCATCTATGAAAAAACTAAATTAAATGTACCTACAACAACAAATGACATTTTCACATTAACTGGCGTAACTGTTGATTGA", "aa": "MAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKISEKNYYSEIKEEQNLVKLYYTDPDTNEEKEFNKENLEKLNIQATTINLNNLMTLNFDDATRKLTITVSYTTKSTPKEKKFELTFAEKSFVELKDTFLAEINLSLPNPCDVTKATDNDIIYEKTKLNVPTTTNDIFTLTGVTVD", "aa_hexdigest": "658942cfc30296747c60896b1b1d6c01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29300.57820000006, "isoelectric_point": 4.775009727478028 }, "id": "IGIGPGHMMC_523", "locus": "GCA000148625_00491" }, { "type": "cds", "sequence": "contig_1", "start": 275966, "stop": 276184, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAAAAGATAAATCAACTAAAACTGCACAAGAATATGTTGAAGAATTTAATGCACTAACTGATCAAGAAAAAGAAGAAAAAATTACATTCACAAATGCAGATTATCAAAATGATTTAGCTGTATTTAAAAACATGGAAGGTTTAAATTACATTAAATATCAATTAGAAGTTGTTGACAATTTTGAAGGAAAAGTTAAATTAGTTGCAAAATGA", "aa": "MDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLAVFKNMEGLNYIKYQLEVVDNFEGKVKLVAK", "aa_hexdigest": "85e0bf3c5a4074136566475b1825720b", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8415.336800000001, "isoelectric_point": 4.61694049835205 }, "id": "IGIGPGHMMC_524", "locus": "GCA000148625_00492" }, { "type": "cds", "sequence": "contig_1", "start": 276308, "stop": 277225, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1", "UniRef:UniRef90_C4XEN1" ], "nt": "ATGAAGAAGAAAATTAAATTAAGTCTTATTTTTTCAAGCGTAACAGCAGGTGTTTTACCTTTAATTTCATCTCAATGTCAAAACACAACAAAAGAAGAAATTAAAGCAGATGAAGACTTTAAACTTTTGTCTATTGCAGATATTAAGAATGCTGATTATGACTCTACAAACAAAGTATATAAGATTAGCACAACTACAAAAGAAGCTGATCAAATTAAAAAAGAAGATATTAAATTTGAAAGTACAACAAAGAAATATACATATATAATTGACAACTTAAAAGCAGATAAAGCTAAGGGTGAATTAGAAATTTTCCTTACTCAAAAAGAAGGAAATAAAGAAATTAAAAAATTCACAATTAAGGTTGTAGGATTTTATGTAGATACTGACAAATTTGACGAAAACATCAAATTTATAACTGGTTTGGACTTAGCAGAAAAAGACAAATACACAGTTAAAGAATACAAAAATCAATTTAATGATCTAGCTAAATTACAAGCTAAATTGTCAATAGCAACTTCAAATAGCAAAGATTTAGCTACTTTCTTAAAAGAATATGAAATATCAATTAAAAGCATAGAAATAGAAGAAGTAAGTGCTTCAAAAGGTGAAGCAAATATTAAAGCAACTTTTGAAAATAAAGCAAAAACTAAATCAAAAGAAATTACAATCAAATTAACTGGATTCAAAAAAGAAACTGAATCGTTACAAGCAACATTAGAAGGTATTTTTGATACTATTGATGTTTTAGAAAACAAAAAAGATTTCAATGTTTTCAACTATACAATTACAGATCTAAGATACTTCAAAAATAAAACACAAGTTGATGTAACAAAAGTATTTAATGAAAAAGGTATTGAAATTAAAAATAAAGAAGCTGATACCTCATCAGGTGAAACTGAACCTTATTCAGGATGA", "aa": "MKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSG", "aa_hexdigest": "ae850df99fd388f214e58ee19d300b84", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 276005, "stop": 277246, "observations": { "mutation": [ 276250, 276283, 276310 ], "stop": [ 276250, 276283, 276310 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN1", "query_cov": 1.0, "subject_cov": 0.5360281195079086, "identity": 1.0, "score": 558.0, "evalue": 3.09e-196, "gene_start": 1, "gene_end": 305, "reference_start": 8, "reference_end": 312, "reference_sequence": "MLMKEKYMKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSGWLSINITFGWKDATKTETYVWSQVVAGFKEIKVNSELEAAFNVDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLSVFKNMEGLNYIKYQLEVVDNFEGKVKLVAKWQQWNQPEYAYNKEITGFVTKETIQQKMAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKIS" }, "paralog": false, "identity": 0.9878934624697336, "score": 785.0, "evalue": 5.32e-288, "description": "Internal stop codon at 276250, 276283, 276310. Nonsense mutation around 276250, 276283, 276310.", "psc": { "uniref90_id": "UniRef90_C4XEN1", "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN1", "UniRef:UniRef50_C4XEN1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1" ], "product": "Lipoprotein-associated type-17 domain-containing protein" } }, "id": "IGIGPGHMMC_525", "locus": "GCA000148625_00493" }, { "type": "cds", "sequence": "contig_1", "start": 277510, "stop": 277728, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGA", "aa": "MTRKTKIGILIGTNIIVTAGLITALAIAPTRNNSNHSNSGTTTSETRKIEVKAKVSVDLSKKIDQLSNPSTE", "aa_hexdigest": "f7a31823f30009d5ceca04e301345f8a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7649.6922, "isoelectric_point": 10.11536350250244 }, "id": "IGIGPGHMMC_526", "locus": "GCA000148625_00494" }, { "type": "cds", "sequence": "contig_1", "start": 279162, "stop": 281171, "strand": "+", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_013354505.1", "SO:0001217", "UniParc:UPI0001A8C1E6", "UniRef:UniRef100_C4XEN3", "UniRef:UniRef50_Q8EUN2", "UniRef:UniRef90_C4XEN3" ], "nt": "ATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAA", "aa": "MSIFKLISPYKPAGDQPEAIKELVENIKDNMQHQVLKGVTGSGKTFTIANVIKEFDRPVLVLSHNKTLASQLYSELKGFFPHNRVEYYVSYFDYYRPEAYIPSSDTYIDKQSQNNKDLDAMRMSAMNAVLTRNDTIIVASVSAIYGALNPSEYYASVFPIEVGMQIKRQEFFKKLVQRNYKRNQIDLELGTFSAKGDAVYIQPADSDEYQIRVDFFGDEIDSIATCDPITKEVIERFSHYTIFPGDAYTVDRSVIDEACDKILVELDERIKYFKANNQLIEAQRIQERVEKDVDSLREFGMCPGIENYARYMDRREAGERPYTLLDYFKGKKPLLFIDESHMMIPQLNGMYHGDYSRKKNLVDYGFRLPSALDNRPLLFKEFENEFDFQTVYISATPDDYELDKTHGLLTKLFVRPTGLLDPKIEIRLSKNQIEDIYDELQRQKDKNERTLILTTTKRLAEELSRYFLEKNEKIAYIHSEHNTFERNEILRKLRKGVYDTVIGINLLREGVDLPEVSLIMVLDADKEGFMRSTKSLIQISGRAARNANGRVIFYADKTTKSMQECIEDNEIKRKMQIAYNKKHNITPKTIIKPIAEPIHGEGIEGEIEFFFKNEKRNQTQSGKKAKEELIKRLREQMNQAAKELDYERAIELRDIILELQAGKDKKVEE", "aa_hexdigest": "dbfa3505caad1d745101853c8b8ae6bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1E6", "ncbi_nrp_id": "WP_013354505.1", "uniref100_id": "UniRef100_C4XEN3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1E6", "RefSeq:WP_013354505.1", "UniRef:UniRef100_C4XEN3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEN3", "uniref90_id": "UniRef90_C4XEN3" }, "psc": { "uniref90_id": "UniRef90_C4XEN3", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_Q8EUN2", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUN2" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "IGIGPGHMMC_527", "locus": "GCA000148625_00495" }, { "type": "cds", "sequence": "contig_1", "start": 281174, "stop": 281416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4", "UniRef:UniRef90_C4XEN4" ], "nt": "ATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGA", "aa": "MRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVN", "aa_hexdigest": "da113e026a68a00f28314dbbeadff8ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 281168, "stop": 281680, "observations": { "mutation": [ 281414 ], "stop": [ 281414 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN4", "query_cov": 1.0, "subject_cov": 0.47058823529411764, "identity": 1.0, "score": 156.0, "evalue": 3.67e-47, "gene_start": 1, "gene_end": 80, "reference_start": 3, "reference_end": 82, "reference_sequence": "MKMRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVNWAVNFIKDLKLELNKVNDIEGFLDLVLTQKNQKLIKQKLSQIFKFVNLDGNVKDIIIIYDLICFIGLLVNLTNTEFELLPYDACNIIS" }, "paralog": false, "identity": 0.9882352941176471, "score": 329.0, "evalue": 9.55e-118, "description": "Internal stop codon at 281414. Nonsense mutation around 281414.", "psc": { "uniref90_id": "UniRef90_C4XEN4", "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN4", "UniRef:UniRef50_C4XEN4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9398.959099999995, "isoelectric_point": 9.380746269226073 }, "id": "IGIGPGHMMC_528", "locus": "GCA000148625_00496" }, { "type": "cds", "sequence": "contig_1", "start": 281696, "stop": 282190, "strand": "+", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "nt": "ATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGA", "aa": "MSAKEEIIVKGAREHNLKNVSVTLPKNKLIVFTGLSGSGKSSLAFNTIYEEGRRKYVDSLSNYARLFLGGTSKPDVDSIDGLSPSISIEQKTTHNNPRSTVGTVTEIYDYFRLLFARIGKPFCPHHNIEITSQTNKDIIDSVFNLKDGSKLYILAPVVDGEKGT", "aa_hexdigest": "9d7e153b83907ee5c6a8c1cceb991b4e", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI0021D31D26", "query_cov": 0.9817073170731707, "subject_cov": 0.8944444444444445, "identity": 0.6459999999999999, "score": 218.0, "evalue": 6.86e-70, "valid": false, "uniref50_id": "UniRef50_A0A848YIN1" }, "pscc": { "uniref50_id": "UniRef50_A0A848YIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "product": "UvrABC system protein A" }, "genes": [], "id": "IGIGPGHMMC_529", "locus": "GCA000148625_00497" }, { "type": "cds", "sequence": "contig_1", "start": 282323, "stop": 282592, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGA", "aa": "MVLNEENRNRISEALSVAVEYGKGIVKVETTDDEVKTFSKLHSCVYKDFEMPKIETKLFSFNSPYGMCQNCKGLGVDLKADFDALTPEP", "aa_hexdigest": "fc551f295422ab42edea8ee30980c0e5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10011.31079999999, "isoelectric_point": 4.910002326965332 }, "id": "IGIGPGHMMC_530", "locus": "GCA000148625_00498" }, { "type": "cds", "sequence": "contig_1", "start": 282902, "stop": 284518, "strand": "+", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "ATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATCAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAA", "aa": "MGTFECSTCHGSRLNEYALAVRINGYNIEDFSRMSVEDALITIENLKLNEEETKISTLIINELINRLSFLKNVGLNYLTLNRNAETLSGGESQRIKLATQIGSNLTGVLYVLDEPSIGLHQKDNDRLIKTLKSMVEIGNTLIVVEHDEETIRSADYIVDIGPKAGIHGGEIIAQGTLEDIIKEPKSITGKYLSGEMKIEVPRVRRSGNGKVVTIFQAAENNLKNIDVKFPLGKFIGVTGLSGSGKSSLVNEILVKGIESVLGHKTGKIGKFKSISGLNQIDKIVPISQSPIGRTPRSNPATYTSVFDDIREIFASVEESRARAYSRSRFSFNVPGGRCEKCQGDGSIKIEMHFLPDVYVPCDECDGQRYNRETLEIKYKNKNIAQVLDMTADEALEFFSTRANIRIKLETLVAVGLGYIKLGQSSTTLSGGEAQRVKLATYLQKKATGKTIYVLDEPTTGLHSYDVANLLKVLNKIVDNGDTVVVIEHNLDVIKCCDYIIDLGPEGGVNGGRVVATGTPEQVAENENSYTGQYLKMIL", "aa_hexdigest": "c9f1e1ab50166988e5f6cd6d899b9299", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XVF5", "query_cov": 1.0111524163568772, "subject_cov": 0.9963369963369964, "identity": 0.691, "score": 757.0, "evalue": 4.79e-271, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "IGIGPGHMMC_531", "locus": "GCA000148625_00499" }, { "type": "cds", "sequence": "contig_1", "start": 284825, "stop": 284929, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGA", "aa": "MDVATKKYVNKYPYKVRKYGPITERRDYSVTKIT", "aa_hexdigest": "5c7091208219028974a42cfdee7e2a1e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4112.751300000001, "isoelectric_point": 9.917123222351073 }, "id": "IGIGPGHMMC_532", "locus": "GCA000148625_00500" }, { "type": "cds", "sequence": "contig_1", "start": 285074, "stop": 286027, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "TTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTGGAAGTTTTATCATCAATGGTAGCGAAAAAGTTATCGTTAGTCAATTAATTAGATCACCTGGAGCTTACTTTGGACGTGGCGTTAGAAATAAACAATCTGATGACCTTTTCAACAAGGTAGAAATTTTACCTCGTATTGGTTCTTGGGTTGAAATCTCTCACAAGGTTACAACTAAAACACCAGATAGCGTAAAAATTAAAATCGATAAAAACAAAAATGTTAACTTAGTAACTTTCTTAGGTTCACTTGGTTTAGATCGCCAAATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGA", "aa": "MEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSGGSFIINGSEKVIVSQLIRSPGAYFGRGVRNKQSDDLFNKVEILPRIGSWVEISHKVTTKTPDSVKIKIDKNKNVNLVTFLGSLGLDRQMIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKK", "aa_hexdigest": "7fc93c917cd2c8d1eea12740016aa66a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 130, "aa_cov": 0.38801261829652994, "hmm_cov": 0.9230769230769231, "evalue": 1.7433282692476252e-07, "score": 27.07325553894043, "start": 108, "stop": 230 } ], "seq_stats": { "molecular_weight": 35709.0328, "isoelectric_point": 9.555520057678223 }, "id": "IGIGPGHMMC_533", "locus": "GCA000148625_00501" }, { "type": "cds", "sequence": "contig_1", "start": 286028, "stop": 287986, "strand": "+", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_Q98Q23", "UniRef:UniRef90_C4XEN6" ], "nt": "ATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGACCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGA", "aa": "MDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQ", "aa_hexdigest": "f4a833893f9c61d3741c0dda25f49b89", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028FF9953", "query_cov": 0.8634969325153374, "subject_cov": 0.9825479930191972, "identity": 0.51, "score": 563.0, "evalue": 1.63e-192, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A7V5ZEA3", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 285728, "stop": 288289, "observations": { "mutation": [ 286025, 287984, 288179 ], "stop": [ 286025, 287984, 288179 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN6", "query_cov": 1.0, "subject_cov": 0.5370675453047776, "identity": 1.0, "score": 1278.0, "evalue": 0.0, "gene_start": 1, "gene_end": 652, "reference_start": 411, "reference_end": 1062, "reference_sequence": "MMRKKEYYCMDVATKKYVNKYPYKVRKYGPITERRDYSVTKITWNVPDFLVTSKESFEWFKKTGIEESLREHYPITATNKKVSLEYIPNSADLEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSGWSFIINGSEKVIVSQLIRSPGAYFGRGVRNKQSDDLFNKVEILPRIGSWVEISHKVTTKTPDSVKIKIDKNKNVNLVTFLGSLGLDRQMIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKKWMDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQWLIDPITGNKLDKPVSVGVMYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMETWAIESYGATNVLQEILTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE" }, "paralog": false, "identity": 0.9964830011723329, "score": 1669.0, "evalue": 0.0, "description": "Internal stop codon at 286025, 287984, 288179. Nonsense mutation around 286025, 287984, 288179.", "psc": { "uniref90_id": "UniRef90_C4XEN6", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q98Q23", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN6", "EC:2.7.7.6", "UniRef:UniRef50_Q98Q23", "KEGG:K03043", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q23" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "IGIGPGHMMC_534", "locus": "GCA000148625_00502" }, { "type": "cds", "sequence": "contig_1", "start": 288041, "stop": 288181, "strand": "+", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79", "UniRef:UniRef90_A0A920JZ79" ], "nt": "ATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGA", "aa": "MYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMET", "aa_hexdigest": "6afa928b5687044909329d0b39d090c4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 288041, "stop": 288231, "observations": { "deletion": [ 288212 ], "mutation": [ 288179 ], "stop": [ 288179 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A920JZ79", "query_cov": 0.9782608695652174, "subject_cov": 0.703125, "identity": 0.8220000000000001, "score": 84.0, "evalue": 1.72e-20, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MYIIKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGATNVPSRSVDS" }, "paralog": false, "identity": 0.746031746031746, "score": 100.0, "evalue": 1.28e-29, "description": "Internal stop codon at 288179. Frameshift due to deletion around 288212. Nonsense mutation around 288179.", "psc": { "uniref90_id": "UniRef90_A0A920JZ79", "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A920JZ79", "UniRef:UniRef50_A0A920JZ79" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79" ], "product": "DNA-directed RNA polymerase" } }, "id": "IGIGPGHMMC_535", "locus": "GCA000148625_00503" }, { "type": "cds", "sequence": "contig_1", "start": 288224, "stop": 288442, "strand": "+", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "nt": "TTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAA", "aa": "MTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE", "aa_hexdigest": "43872484d4cbd0d223949c4907053db8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8L4", "query_cov": 0.9305555555555556, "subject_cov": 0.8933333333333333, "identity": 0.537, "score": 74.7, "evalue": 3.08e-16, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "uniref50_id": "UniRef50_A0A3B0P8L4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "product": "DNA-directed RNA polymerase subunit beta" }, "genes": [], "id": "IGIGPGHMMC_536", "locus": "GCA000148625_00504" }, { "type": "cds", "sequence": "contig_1", "start": 288435, "stop": 288530, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGA", "aa": "MSNFIDKNKLNKDREIEKITLSLATKDDVLQ", "aa_hexdigest": "14a851522361cc5d273f42f8abbf6053", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3621.1214, "isoelectric_point": 6.026888465881346 }, "id": "IGIGPGHMMC_537", "locus": "GCA000148625_00505" }, { "type": "cds", "sequence": "contig_1", "start": 289095, "stop": 289511, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGCCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGA", "aa": "MGKDYGIDFYEYNEIIHEYSDAIISTGSKAIEYLLEHVDLQKEAQLIEDEIKKINRSIKDKNHTGTRGQERSKLYKRLAVINAFINSGQDPKSMLIYDLPVIPADLRPLVQLDGGRHSTSDINELYRRIIIRNNRLKK", "aa_hexdigest": "c74c7a2c8f2075b913fff079790609c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 130, "aa_cov": 0.8623188405797102, "hmm_cov": 0.9615384615384616, "evalue": 1.0041259821580977e-22, "score": 76.9033432006836, "start": 20, "stop": 138 } ], "seq_stats": { "molecular_weight": 16003.04999999999, "isoelectric_point": 7.908546257019044 }, "id": "IGIGPGHMMC_538", "locus": "GCA000148625_00506" }, { "type": "cds", "sequence": "contig_1", "start": 289530, "stop": 289808, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "ATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGATGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGA", "aa": "MLIKQNEYRMIQEAVDSLIDNARKKPNPVTSKDSRPLKSISDALTGKKGRFRQNLLGKRVDYSGRSVIVVGPELKMHQVGIPREMAAKLFEP", "aa_hexdigest": "cb37aaaa2b3672a596c9a0d2891c39d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030FE3613", "query_cov": 0.8913043478260869, "subject_cov": 0.9647058823529412, "identity": 0.6459999999999999, "score": 109.0, "evalue": 1.75e-29, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "IGIGPGHMMC_539", "locus": "GCA000148625_00507" }, { "type": "cds", "sequence": "contig_1", "start": 289932, "stop": 290789, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25", "PFAM:PF04983.23" ], "nt": "GTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGCAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAACGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGTGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGA", "aa": "MLLNRAPTLHRLSIQAYEPVLIRGKAIKLHPLSCTPFNADFDGDQMAVHVPISDEAVREAKELMLASRNILGPKDGEPIINPGQDIILGLYYLTQEATDDKVIGEGRYFGSYDEMMTAYENKLVSLHARVVLPISEINKRVIPADGQDRYIVSTVGKFIFNSIFPDSFEFIFGKYVERNIKEVDGKKVISEKEVLHTSGDKNQLKNYLLPYGMNFPEIIKNMPLNLPLGKKDIAKIVRRIFDRYVAIVTMEDLAAIISDLNSETINNLFDNCANLVDYNKNTIPV", "aa_hexdigest": "4491e408f55da7532f23bf48b0a78893", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 64, "aa_cov": 0.22456140350877193, "hmm_cov": 1.0, "evalue": 1.9154379014215938e-26, "score": 89.2982177734375, "start": 2, "stop": 65 }, { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 183, "aa_cov": 0.631578947368421, "hmm_cov": 0.73224043715847, "evalue": 4.6921725367470556e-23, "score": 77.87889862060547, "start": 69, "stop": 248 } ], "seq_stats": { "molecular_weight": 32178.772000000023, "isoelectric_point": 5.638109779357909 }, "id": "IGIGPGHMMC_540", "locus": "GCA000148625_00508" }, { "type": "cds", "sequence": "contig_1", "start": 290946, "stop": 291155, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGA", "aa": "MADVLDKLKALGYHYSTISGTTISISDVTTLPSTKDKIKEGDKYIEQLRDYFEQGLLTDDERYALTIQK", "aa_hexdigest": "748e7fd156653e0795e49424036d87ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7845.750899999997, "isoelectric_point": 4.898918724060058 }, "id": "IGIGPGHMMC_541", "locus": "GCA000148625_00509" }, { "type": "cds", "sequence": "contig_1", "start": 291228, "stop": 291995, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCATGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTGTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGA", "aa": "MMFTSGARGNSSNFVQLAGMRGLMNNNTKILKADAENERVVRSTVEIPVKSSFLDGLTAYEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQGIVVREDDCGTDYGFEVKDILDTKTNTIIESLGERIEGRFSNVPIYHPRTKEMILDADQLITPQIAEEIVKAGIKSVEIRSVLSCYTKNGVCKKCYGKDLALNRVVNIGEAVGVVAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFGRLMELIDAYDSP", "aa_hexdigest": "b214c0c8d4d605aa3f50b5788d288fe7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20784313725490197, "hmm_cov": 0.9821428571428571, "evalue": 4.443521869939427e-12, "score": 41.91604232788086, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7647058823529411, "hmm_cov": 0.5948717948717949, "evalue": 1.1826871794851718e-46, "score": 155.34707641601562, "start": 56, "stop": 250 } ], "seq_stats": { "molecular_weight": 27669.165000000005, "isoelectric_point": 5.736384391784668 }, "id": "IGIGPGHMMC_542", "locus": "GCA000148625_00510" }, { "type": "cds", "sequence": "contig_1", "start": 292275, "stop": 292910, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAA", "aa": "MKEVQRLYRLQGITISDKYIEIIIRQMLSKIVITDPGDSKFFSGNLVDIFAYQRESARLISQGKKPPFGEVKIKGAKQTPLLSDSFLAAASYQETPKILVNASIAAQSDKLEGLKENIILGHKIPAGTNCNFEEKGKYDIRDPRSYFQSKFAENSDFPNPNFEDNLNNLQSQLDEFNSVDPEELAIEEEMGIEEVYEDDYYSDLDENNDNN", "aa_hexdigest": "a21de706efa36cc86eee2f4bf5c11a92", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23991.430699999997, "isoelectric_point": 4.520939445495605 }, "id": "IGIGPGHMMC_543", "locus": "GCA000148625_00511" }, { "type": "cds", "sequence": "contig_1", "start": 293577, "stop": 294047, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGA", "aa": "MKIKLKQTLIILSTTLVGTIALATGLSFLPTKDKTIPRGKQSFNDDHKNYNDPNIPNLPGNNVPTKNPKPTLVLKNRPKSANAITKENSIKYLALGDSISAGFNAQLDKDYPGQLVNSKIEGLSFPTYLASFFQQLADKKLESFDNKAVSGTTFKQ", "aa_hexdigest": "ff68db457853b9f942c62f73e6c18645", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17097.40229999998, "isoelectric_point": 9.726296806335448 }, "id": "IGIGPGHMMC_544", "locus": "GCA000148625_00512" }, { "type": "cds", "sequence": "contig_1", "start": 294096, "stop": 294596, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGCGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGA", "aa": "MAELNKYFGTDLVNIKNDLIAKIKNANLITVTLGANDFLKDLLFKMVSNMPILDLIKQIKNKNLDYNMLVSHFDQVFRTLFEQLENRQKQFIQTIKSLNDKVNINFIAYPLPLTFIMDLIDKAINKGDTNNALSIGQTILDLLNKKLKYTAISQKTYYINPYQSSF", "aa_hexdigest": "24568c701aac3ed95bab71f0e0a0a1c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19174.1777, "isoelectric_point": 9.280111122131348 }, "id": "IGIGPGHMMC_545", "locus": "GCA000148625_00513" }, { "type": "cds", "sequence": "contig_1", "start": 294975, "stop": 295076, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGA", "aa": "MFKSKIYETIDPNRKLEKFMHRDDYANQKSLRD", "aa_hexdigest": "d0f0b5888402d395d3ba5483dc28b33d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4118.6532, "isoelectric_point": 9.222669792175292 }, "id": "IGIGPGHMMC_546", "locus": "GCA000148625_00514" }, { "type": "cds", "sequence": "contig_1", "start": 295356, "stop": 295901, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTCGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGA", "aa": "MLKLVKSFYNENISKYISQNDLASLLKLVLNTKSIKSAVGDLVVELINSAPEYAKCKSINELFNVFISNKNNQNTIKKFIESIVNDLLSQNEFKQIVSRLASKIIKQYPELFKDIDENNLTNLIYESLSLGGEINNALGLVTKFVNSLLNELTTTNIKEFKFDNVIKNTLTEIKTLFDKDN", "aa_hexdigest": "10cc769436aa87017ce8b826c6c254c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20575.448400000016, "isoelectric_point": 7.697386360168458 }, "id": "IGIGPGHMMC_547", "locus": "GCA000148625_00515" }, { "type": "cds", "sequence": "contig_1", "start": 296133, "stop": 296516, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGA", "aa": "MSNLISQLVSDLLKTNQTQLMDVTNFFDAIKVIFKNFVNTSAYDYISPLIKKVFEFPEAKDLFKKAMAKIPAQEAKNLNDRDVADIVSFVLDNNEFKDILQDFLVKGIFGDAITIEKLKDANNIIKS", "aa_hexdigest": "26b8481b3cb62cff42b8bd457e13f4a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14425.519299999976, "isoelectric_point": 5.098309898376464 }, "id": "IGIGPGHMMC_548", "locus": "GCA000148625_00516" }, { "type": "cds", "sequence": "contig_1", "start": 296676, "stop": 296864, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGA", "aa": "MENFIGDFLTIIPSLDEEFRFIKDVLTKIFNELKATGKAIDFKKVMSEFAKCIKNKLFNKND", "aa_hexdigest": "0255e34fa769216ded9bbcf6fc28bc14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7248.440299999999, "isoelectric_point": 7.8331182479858406 }, "id": "IGIGPGHMMC_549", "locus": "GCA000148625_00517" }, { "type": "cds", "sequence": "contig_1", "start": 296913, "stop": 297815, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09204.15" ], "nt": "ATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGA", "aa": "MNKHQAFLRKFVKNILVYVSEDIALGKKLFKSLSEKAHKIIDKHIDENDFNNMFIDLFQRTEGLQRIIDNILDSLFSDIDSYQNANSLIDILKIYVMPQDRADRLAKNLEDVLKEFLKNEALHKLLRGLFEENVAPYGIDPKSPGNKALVDDLLANLHTFFVDLKILPNLANSMVDSFKKYNNFNEIIKNIEPILVNGLKLKEYSFVATILKNNIVNKHKESIRDDINKVIAGITSKINLVNKFIDDFNLIKPIKDMGLSETKARDFLTQVIQSQNLKTIIDVFVKELLDKNLEYAQLNS", "aa_hexdigest": "43ea93ea515c6d8e67721c724751e3c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09204.15", "name": "Colicin_immun", "length": 54, "aa_cov": 0.16, "hmm_cov": 1.0, "evalue": 1.4689558335623433e-06, "score": 24.79534912109375, "start": 37, "stop": 84 } ], "seq_stats": { "molecular_weight": 34552.618900000045, "isoelectric_point": 7.029585075378416 }, "id": "IGIGPGHMMC_550", "locus": "GCA000148625_00518" }, { "type": "cds", "sequence": "contig_1", "start": 297837, "stop": 297953, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGA", "aa": "MSSPSALIIKGTIKQYITELFEKNEEVAFTIGKLIANG", "aa_hexdigest": "02765bf2b02be693f886f10db481de01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4155.807500000001, "isoelectric_point": 6.049339866638183 }, "id": "IGIGPGHMMC_551", "locus": "GCA000148625_00519" }, { "type": "cds", "sequence": "contig_1", "start": 298080, "stop": 298526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGA", "aa": "MKRINTYGFEKLIDNLRDAAKEGALKFISEGGKVRLSKIFDQIPLIRDLLAKVPQKEFSDFLNLAFSSTPKNDTEGLFKVMFNSDKGTSKFEVGIGASGIIDILKGKVGDLVSAIASPLVKEFFKQASEQNYSDHNTLRKTNTGFQAL", "aa_hexdigest": "53a8667c8547f80e4d9fd11db7de92c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16379.613899999986, "isoelectric_point": 9.272439384460448 }, "id": "IGIGPGHMMC_552", "locus": "GCA000148625_00520" }, { "type": "cds", "sequence": "contig_1", "start": 298599, "stop": 298772, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGCTGAAGCTTACTTAATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGA", "aa": "MTAEAYLMDGFVKAFKDNVKDYKTALLAKYGDKSLIGFNNSFDPLDYYLSGMTTVNY", "aa_hexdigest": "9a9d5de8cad1f81d6b402a827c8a7781", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6460.273900000002, "isoelectric_point": 4.919437599182127 }, "id": "IGIGPGHMMC_553", "locus": "GCA000148625_00521" }, { "type": "cds", "sequence": "contig_1", "start": 298962, "stop": 299243, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGA", "aa": "MNKKEFKKRLIEYCETEGLSRYEENVAHALVKNINSKNFEVNYDNFGSLILHKKSKVKDAPKFMIAAHMDEVGYLVRQIHEDGQILLSSVGGI", "aa_hexdigest": "9358c8ae01ae0baa5ada4f3e8ff7af1a", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10670.157199999998, "isoelectric_point": 7.862451362609863 }, "id": "IGIGPGHMMC_554", "locus": "GCA000148625_00522" }, { "type": "cds", "sequence": "contig_1", "start": 299334, "stop": 300047, "strand": "+", "frame": 3, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAA", "aa": "MEAEARNRAVTNKEIYADFGFKNKEEALKKNVQIGDRVLMSGETINFDNENLVGAKAMDNRAGVTTLEYIAHQLKDLDLAVDLYLVGTVQEEVGTRGAKTSVTVIDPQIAIALDTTSTHDTIGTIAGTTALGKGAAIRIMDGAMMANPRLVELMCKIGRKEKIKHYKFVAMGGGTDASALQYAKGGAATLTISLAQRYLHSPIGVCDIEDLMAAGDLIVETVKAINPKVYDQEIKYH", "aa_hexdigest": "2bf573c54280bf9338c038ebe2726a29", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8649789029535865, "subject_cov": 0.9715639810426541, "identity": 0.649, "score": 255.0, "evalue": 3.94e-83, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "IGIGPGHMMC_555", "locus": "GCA000148625_00523" }, { "type": "cds", "sequence": "contig_1", "start": 300150, "stop": 300350, "strand": "+", "frame": 3, "gene": null, "product": "putative protein family (UPF0154)", "db_xrefs": [ "KEGG:K09976", "SO:0001217", "UniRef:UniRef50_Q98R64", "UniRef:UniRef90_A0A3P8KMY9" ], "nt": "ATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAA", "aa": "MAILFFLMGGFIAFIVSKKMFEKQIKENPPITEKMIRAMFLQMGRKASESQIKAVMRSMTNAKDEK", "aa_hexdigest": "6a8e6265ae53ebfde947e8b0bc752ba5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3P8KMY9", "query_cov": 0.9848484848484849, "subject_cov": 0.8125, "identity": 0.9229999999999999, "score": 114.0, "evalue": 7.78e-32, "valid": true, "product": "Uncharacterised protein family (UPF0154)", "uniref50_id": "UniRef50_Q98R64", "kegg_orthology_id": "K09976" }, "pscc": { "uniref50_id": "UniRef50_Q98R64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R64" ], "product": "UPF0154 protein MYPU_1460" }, "genes": [], "id": "IGIGPGHMMC_556", "locus": "GCA000148625_00524" }, { "type": "cds", "sequence": "contig_1", "start": 300652, "stop": 301398, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAGTTATCCTAGAACTGATAGTACTAGATTGAGTCAACATTTCTTAAATATTGCTAGAAGTTATATTAAAAATAAATTCGGTGAAGAATATATTCTTCAAGAAATCAAAGGCTTTGCAGGCGATCAAGATGCCCATGAAGCTATTAGACCAACTGATATAAACTTACTGCCTGAAGTTGCTAAAGCTAAATTTAATTTAGATAATAGTGAATACATTGTTTATAAGCTAATTTATGAACACACACTTATGTCTTTAATTAATCCACCAATTAAAGCTAATAAAACTTATACCTTTAAAAAAAATAAGTTAACTTTTAAAGCTAATGTAAGTAAAACCATATTCCCAGGTTACTATCTTGTTAAAAATGAAAATGAAGATATTGAAGATCCTAATTATCAAATACAACAAAAAGTGGATGTTTTAGAATATCAAATAACAAATCATGAAACTAAACCACCAGCTCGTTACACTGAAGGTTCATTAATTGAAGCTTTAGATAATATTAAAGTTGGACGTCCTTCAACTTTTGCAACTACTGTTGCAGTAAACTTGGAAAGAAAATATTTTGAAAATATTGATAAAAGTTTACATCCTACAGAATTTGGCCGTTTAGTTTTAAGTAAATTATTAGATGGTTTTCCAGATATTATTAATGAAGGCTATACAGCTGAAGTTGAAGAACAATTAGACTTAATTGCTTCAAATAAAACTACTGTTCAACCTATTATGTATGATTTTTGA", "aa": "MISYPRTDSTRLSQHFLNIARSYIKNKFGEEYILQEIKGFAGDQDAHEAIRPTDINLLPEVAKAKFNLDNSEYIVYKLIYEHTLMSLINPPIKANKTYTFKKNKLTFKANVSKTIFPGYYLVKNENEDIEDPNYQIQQKVDVLEYQITNHETKPPARYTEGSLIEALDNIKVGRPSTFATTVAVNLERKYFENIDKSLHPTEFGRLVLSKLLDGFPDIINEGYTAEVEEQLDLIASNKTTVQPIMYDF", "aa_hexdigest": "ff4d88f72bc9d24d9bff51216862bea4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28586.06860000002, "isoelectric_point": 5.490498924255371 }, "id": "IGIGPGHMMC_557", "locus": "GCA000148625_00525" }, { "type": "cds", "sequence": "contig_1", "start": 301609, "stop": 301848, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAGAATTATTGGTTTCAAATTAAGCCAATTAATGCAAAAGAAAATTTCAAATAGTCCTCGTACTCCAAGTGCAGGACGTGTACAATCAATTGCTCTTAAATTAGTAGTTGATCGTGAAGATGAAATTAATGCATTTGTGCCTCGTGGTTATCACAAAGTTGAAGCTAAATTAAATGAAACATTAAGTGCTTCTATTTATATAGATAAACATCCAGCAGCAGAAAAAAACTGA", "aa": "MLDRIIGFKLSQLMQKKISNSPRTPSAGRVQSIALKLVVDREDEINAFVPRGYHKVEAKLNETLSASIYIDKHPAAEKN", "aa_hexdigest": "3c96d2e6c57863e33521765f8e9558d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8877.174699999994, "isoelectric_point": 9.628240394592286 }, "id": "IGIGPGHMMC_558", "locus": "GCA000148625_00526" }, { "type": "cds", "sequence": "contig_1", "start": 302134, "stop": 302289, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTAGTTATAGTGGAATCTCCAAACAAAGTTGAGACAATTAAAAAATATTTAGGAAGCGATTATGATGTGATTGCTTCTGTTGGTCATATCGCTATTATGAGTACTAGGAAAGGCGAACAAGGTTTAGGAATTGATTTAGAAAATTGA", "aa": "MNNLVIVESPNKVETIKKYLGSDYDVIASVGHIAIMSTRKGEQGLGIDLEN", "aa_hexdigest": "9faf956af32f225ce53d04ce4d2af35a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5561.301199999999, "isoelectric_point": 5.045847511291503 }, "id": "IGIGPGHMMC_559", "locus": "GCA000148625_00527" }, { "type": "cds", "sequence": "contig_1", "start": 302653, "stop": 302895, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "SO:0001217", "UniRef:UniRef50_C4XEP2", "UniRef:UniRef90_C4XEP2" ], "nt": "ATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGA", "aa": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDI", "aa_hexdigest": "acdce77b6e5843fd69c6a76237c7d384", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 302653, "stop": 303126, "observations": { "mutation": [ 302893 ], "stop": [ 302893 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP2", "query_cov": 1.0, "subject_cov": 0.5095541401273885, "identity": 1.0, "score": 152.0, "evalue": 1.16e-45, "gene_start": 1, "gene_end": 80, "reference_start": 1, "reference_end": 80, "reference_sequence": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDIWRHLAINLDSERGYGKVKKESHRAKYEEKKEFKKSNKSAEACEKVAEEKAAKKPRSAKKESQEKEVKPRQSKAKKVE" }, "paralog": false, "identity": 0.9681528662420382, "score": 247.0, "evalue": 1.46e-85, "description": "Internal stop codon at 302893. Nonsense mutation around 302893.", "psc": { "uniref90_id": "UniRef90_C4XEP2", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_C4XEP2", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP2", "UniRef:UniRef50_C4XEP2", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP2" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "IGIGPGHMMC_560", "locus": "GCA000148625_00528" }, { "type": "cds", "sequence": "contig_1", "start": 303154, "stop": 303303, "strand": "+", "frame": 1, "gene": null, "product": "Single strand binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7", "UniRef:UniRef90_A0A3P8MEY7" ], "nt": "ATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGA", "aa": "MNKVLLVGRISNDIRMYRTNSGISYARTSIAVNRRSSNDKITDFIPIVA", "aa_hexdigest": "624a0c9ac922e317e98f68c319adc8e4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 303154, "stop": 303329, "observations": { "deletion": [ 303289 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEY7", "query_cov": 0.9183673469387755, "subject_cov": 0.7758620689655172, "identity": 0.9109999999999999, "score": 85.1, "evalue": 5.86e-21, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MNKVLLVGRISNEIRMYRTNSGIAYARTSVAVNRRSSNDKITDFVSYCRMKKFSRISK" }, "paralog": false, "identity": 0.896551724137931, "score": 101.0, "evalue": 4.22e-30, "description": "Frameshift due to deletion around 303289.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEY7", "product": "Single strand binding protein", "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEY7", "UniRef:UniRef50_A0A3P8MEY7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7" ], "product": "Single strand binding protein" } }, "id": "IGIGPGHMMC_561", "locus": "GCA000148625_00529" }, { "type": "cds", "sequence": "contig_1", "start": 303330, "stop": 303482, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGTTCTTTGTTCTCTTACTGAACGAGGTTCAAGTAATGAAATATTGTCAACTGTAACTTCATATGATCAAACTGTTCCATTTTGTCCTTCATATTGTGATGAAGTAAATGAACCTTCAATTGAAACAAGAGCTCCTTTTGAAGCATAG", "aa": "MPVLCSLTERGSSNEILSTVTSYDQTVPFCPSYCDEVNEPSIETRAPFEA", "aa_hexdigest": "b4499a9fb398002494902aaefd897972", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5515.054299999999, "isoelectric_point": 4.0500284194946286 }, "id": "IGIGPGHMMC_562", "locus": "GCA000148625_00530" }, { "type": "cds", "sequence": "contig_1", "start": 303705, "stop": 303959, "strand": "+", "frame": 3, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02963", "RefSeq:WP_013354516.1", "SO:0001217", "UniParc:UPI0001E3308C", "UniRef:UniRef100_C4XEP4", "UniRef:UniRef50_A5IY67", "UniRef:UniRef90_C4XEP4" ], "nt": "ATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAA", "aa": "MVFNNKKKNNFRRRRCETCDQKLTYVDYKDVEFLSKFITATGQIKPHSATGACAKDQRKIANAIKRARFMALIPYSKDRVRLVK", "aa_hexdigest": "bb4e0704f05956295c561802130d9245", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3308C", "ncbi_nrp_id": "WP_013354516.1", "uniref100_id": "UniRef100_C4XEP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3308C", "RefSeq:WP_013354516.1", "UniRef:UniRef100_C4XEP4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEP4", "uniref90_id": "UniRef90_C4XEP4" }, "psc": { "uniref90_id": "UniRef90_C4XEP4", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_A5IY67", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY67" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "IGIGPGHMMC_563", "locus": "GCA000148625_00531" }, { "type": "cds", "sequence": "contig_1", "start": 304052, "stop": 304222, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGA", "aa": "MNFEKIFICTTDTVTGIGGPICQETLDALYYLKKRPKSKKIMILVASLKQAQSFKE", "aa_hexdigest": "b85bc97ab39d8c249103dfa7341fc723", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6340.497000000001, "isoelectric_point": 9.301772499084471 }, "id": "IGIGPGHMMC_564", "locus": "GCA000148625_00532" }, { "type": "cds", "sequence": "contig_1", "start": 304301, "stop": 304498, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGA", "aa": "MPNNKKLLEFLEKNGPMYVTSANVSTQEPIDIKDAHKVFPEVKNVYNFGKGKGVASHIIDAQSNE", "aa_hexdigest": "21ce2223b919c584756e23f5caaa5add", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7232.124700000004, "isoelectric_point": 6.7218019485473635 }, "id": "IGIGPGHMMC_565", "locus": "GCA000148625_00533" }, { "type": "cds", "sequence": "contig_1", "start": 304532, "stop": 305674, "strand": "-", "frame": 1, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6", "UniRef:UniRef90_C4XEP6" ], "nt": "ATGGAAAGCATTAAAGAAGGCAAGAAAATTTCACCTGACCAATTAATTGAAGAAGCTTTTAAATCAGATACAAATAAAATAGTTGAATCAATGGGTAAAGAATTAAAAGAAAAATATCCAAATTGTTTAGCTTTTTCGCCTATTGTTGTTGTCGAAGATATTACTAAAGATAAAGCTAAAGCTGTTGTTACAATTGTAATTTATCCAAAAGCTGAATTCGATAAAATTGACATAAATATTAAACCAGATGGTAAATATGAAATGGTGACAAAAGGTGCTATTGATGAAATTTACAAAAAATTTGTTGATGATTATCCTTTACTTAAAGAAGTTGAAGACCAATCAAAAGAAAATGATTATGTTAGATATAATATTTCAGTTTCTAAAGACAAAAAAGAAATATTGTCACGTAAAGATTTAGATAAAAAAATTGTAAAAGATGAAAATCCTAACTCAATTAATAGTCAATTAATGAATCGTAAAGTTAATGAATCATTTTCAGTAAATACACCAGAAAATGAAAACATTAAAATTACAGTTACTAAAGTTTTTCGTCCAATTAAAACTAAATTAACAAATGACAACATAAAAGAAATCAAAATTGAAGGCATTAATACTCTTGATGATCTTTATAATCAATTGTCAATGAATTTGAAAAATGAAATGGCTTCAAGTTATTTATTAGATTACATGAAAAAAGCAATTGAAACAGTTGGAAATAAATATGGTTTTGATTTACCACCTATTTTATTAGATACACAAGTAAATGATTTTATTAAAAGTAAATTAGCAATGTTACCTCCAGATAAAGCTAATCTATTAAGAAATGCAATAGAAAATAAACAAAAAGATGGTTATGAAATTTTTGATTTAGCAAAAACAAATGTTCGTAATTCTCTTTTAGACGCAATTATTGAATTTACTTTTTCATTGTTAATTAAAAACAAAATTACAGAAAAAGAAGCAGAAGAATTTTACAAATCAATTAAAGAAAAAACTTTAATTTCAACTGGCTCAAATGAATTAACAAAAGCTCAATCTGATGTTATTTTAGCTAAACAAAAAGCAGCTCTTAACTTGCTTTATCTTAATGATAAAGAAAGTTATGAAGCTGTTTCAAAAGATTTAGAATTAACTATTTAA", "aa": "MESIKEGKKISPDQLIEEAFKSDTNKIVESMGKELKEKYPNCLAFSPIVVVEDITKDKAKAVVTIVIYPKAEFDKIDINIKPDGKYEMVTKGAIDEIYKKFVDDYPLLKEVEDQSKENDYVRYNISVSKDKKEILSRKDLDKKIVKDENPNSINSQLMNRKVNESFSVNTPENENIKITVTKVFRPIKTKLTNDNIKEIKIEGINTLDDLYNQLSMNLKNEMASSYLLDYMKKAIETVGNKYGFDLPPILLDTQVNDFIKSKLAMLPPDKANLLRNAIENKQKDGYEIFDLAKTNVRNSLLDAIIEFTFSLLIKNKITEKEAEEFYKSIKEKTLISTGSNELTKAQSDVILAKQKAALNLLYLNDKESYEAVSKDLELTI", "aa_hexdigest": "b3cd4cf4494135e4138bc6e4039a8be5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEP6", "query_cov": 1.0, "subject_cov": 0.9223300970873787, "identity": 0.997, "score": 712.0, "evalue": 6.16e-258, "valid": true, "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP6" }, "pscc": { "uniref50_id": "UniRef50_C4XEP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6" ], "product": "Trigger factor" }, "genes": [], "id": "IGIGPGHMMC_566", "locus": "GCA000148625_00534" }, { "type": "cds", "sequence": "contig_1", "start": 305780, "stop": 306118, "strand": "-", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C", "UniRef:UniRef90_UPI0024C76683" ], "nt": "ATGAGAGGCTTCTTAGGAAGAATGTTATTTTCAAATGATTCAGTATTTAAAAATGTTAAAGTAACTTCAGGTGGAGAAAAAGCGAGATTGATGTTTTCAAGAATGATGCTTTTAGAATCTAACTTCTTGATTCTAGATCAACCTTTAGATCACCTTGATGCTGAAAGCATTGACAGTGTCATTGCAGGATTAAAAGCTTATAAGGGTGGTGTTATTGTAACAACATATAATAGAGCTTTAGTTAACCAAGTAGCTAATGTTATTGTTGAAATTGCTCCTGATAAAAGTTTTATTTATCATGTAACACTAGATGAATATGAAAAAATCATGAACTATTAA", "aa": "MRGFLGRMLFSNDSVFKNVKVTSGGEKARLMFSRMMLLESNFLILDQPLDHLDAESIDSVIAGLKAYKGGVIVTTYNRALVNQVANVIVEIAPDKSFIYHVTLDEYEKIMNY", "aa_hexdigest": "131c50310a6c256771237e36d0e6796e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 305786, "stop": 306418, "observations": { "mutation": [ 306169, 306181 ], "stop": [ 306169, 306181 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024C76683", "query_cov": 0.9910714285714286, "subject_cov": 0.4847161572052402, "identity": 0.802, "score": 170.0, "evalue": 2.77e-51, "gene_start": 1, "gene_end": 110, "reference_start": 116, "reference_end": 226, "reference_sequence": "MNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKMVIVGEDDIAKTRLLECLYGIKKPTSGTIEWGQTITPSYFPNDNSKFFETDETILEWISKWPLENKEKVNQENDDARMRGFLGRMLFSNDSVFKKVNVTSGGEKFRLMFSRMMLLESNFLILDQPLDHLDTESIDSVIEGVNNYKGGAIFTTYNRAFVNQCANVILELQSPEKSFIFRGTLEEYEQIMLDE" }, "paralog": false, "identity": 0.6919431279620853, "score": 288.0, "evalue": 3.05e-100, "description": "Internal stop codon at 306169, 306181. Nonsense mutation around 306169, 306181.", "psc": { "uniref90_id": "UniRef90_UPI0024C76683", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024C76683", "UniRef:UniRef50_UPI0030841F7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "IGIGPGHMMC_567", "locus": "GCA000148625_00535" }, { "type": "cds", "sequence": "contig_1", "start": 306179, "stop": 306421, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGACTTATGTTGATGAAAAAGGAAATACACTATTCCAAAATGTTTCATTCACTTTAGCTCGTGGTGAAAAAATGGTTTTAATTGGTGAAGATGATATTGCTAAAACTAGATTTTTAGAATGCTTAATTGGTAAGATAAAACCAACAAAAGGCACTATAAAATGGGGCCAAACAATAAAACACACTTATTATCCAAATGAAAATAAAGAATATTTTAAAGAAGATATAACAATTCTAGAATGA", "aa": "MTYVDEKGNTLFQNVSFTLARGEKMVLIGEDDIAKTRFLECLIGKIKPTKGTIKWGQTIKHTYYPNENKEYFKEDITILE", "aa_hexdigest": "fb132871bd886214785d80abd84b6037", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.7, "hmm_cov": 0.9122807017543859, "evalue": 6.050450603910203e-09, "score": 32.64236068725586, "start": 13, "stop": 68 } ], "seq_stats": { "molecular_weight": 9305.617999999995, "isoelectric_point": 6.581352806091309 }, "id": "IGIGPGHMMC_568", "locus": "GCA000148625_00536" }, { "type": "cds", "sequence": "contig_1", "start": 306467, "stop": 306643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3", "UniRef:UniRef90_UPI0021FF81A7", "PFAM:PF12848.13" ], "nt": "ATGAAACAATCAAATGCTAAAAAAGAAGTACAAATTGAAAAACTTAAAGCATTTATTGCTCGTTTTAGCGCCAACGCTTCTAAAAGTAAACAAGCAACCAGTAGAAAAAAATCTTTAGAAAAAATTACTCTTGATGAAATTAAACCTTCTAATCGTAAATATCCTTATATTAATTGA", "aa": "MKQSNAKKEVQIEKLKAFIARFSANASKSKQATSRKKSLEKITLDEIKPSNRKYPYIN", "aa_hexdigest": "bf4955c82b17bcc239a7c115be587dbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 306347, "stop": 306700, "observations": { "mutation": [ 306469, 306676 ], "stop": [ 306469, 306676 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF81A7", "query_cov": 0.9827586206896551, "subject_cov": 0.4830508474576271, "identity": 0.877, "score": 94.0, "evalue": 1.36e-23, "gene_start": 1, "gene_end": 57, "reference_start": 20, "reference_end": 76, "reference_sequence": "MYTGNYSFWKQSSELARELMKQSNVKKEAQMEKLKEFIARFSANASKSKQYTSRKKALEKITLDEIKPSNRKYPFIRWDMNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKW" }, "paralog": false, "identity": 0.7606837606837606, "score": 176.0, "evalue": 1.48e-58, "description": "Internal stop codon at 306469, 306676. Nonsense mutation around 306469, 306676.", "psc": { "uniref90_id": "UniRef90_UPI0021FF81A7", "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF81A7", "UniRef:UniRef50_UPI00220019C3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3" ] } }, "hypothetical": true, "pfams": [ { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 54, "aa_cov": 0.9310344827586207, "hmm_cov": 0.9629629629629629, "evalue": 3.349356885970797e-14, "score": 48.78986740112305, "start": 3, "stop": 56 } ], "seq_stats": { "molecular_weight": 6685.7099, "isoelectric_point": 10.248297309875486 }, "id": "IGIGPGHMMC_569", "locus": "GCA000148625_00537" }, { "type": "cds", "sequence": "contig_1", "start": 306815, "stop": 306937, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGTCAGAATTAACTGCTAATCAAAAAATTAAAGTTTTATTAGCCAAAGCATTATTTGGTAATCCTGATATTTTAATCATGGACGAACCTACTAACCATCTTGACTTGCGTGCAATTAAATGA", "aa": "MSELTANQKIKVLLAKALFGNPDILIMDEPTNHLDLRAIK", "aa_hexdigest": "c70fc17a17af7f3cfe2e7719ba2ec16a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 31, "aa_cov": 0.775, "hmm_cov": 1.0, "evalue": 1.207330154857563e-06, "score": 25.19322967529297, "start": 2, "stop": 32 } ], "seq_stats": { "molecular_weight": 4476.2655, "isoelectric_point": 6.52206974029541 }, "id": "IGIGPGHMMC_570", "locus": "GCA000148625_00538" }, { "type": "cds", "sequence": "contig_1", "start": 307007, "stop": 307393, "strand": "-", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "nt": "ATGATAGAAGTAAATAATCTAAGTAAAATTTTTAGCGACAAAAAATTATTTGAAAATGTTAATTTAAAATTTACAGCTGGAAATACATATGGAATAATTGGAGCCAATGGAGCTGGCAAAAGTACATTTTTAAAAATTTTAGCTGGTGAAATAGAAGCTACCGGCGGCCAAATAATAATTGAAAAAAGCCGAAGACTTAGTGTTTTAAGCCAAGATCATAATGCTTATGACAATATGAATGTAACCGATGTTGTAGTTATGGGAAATACTGATTTATATAAAATCAAACAAGAAAAAGATGCTATTTATGCAAATCCTGAAGCAACAATGGAAGACTATACAAGGGCAGGAGAATTAGAAGAAAAATTCGGCGAATTAGGTGGTTGA", "aa": "MIEVNNLSKIFSDKKLFENVNLKFTAGNTYGIIGANGAGKSTFLKILAGEIEATGGQIIIEKSRRLSVLSQDHNAYDNMNVTDVVVMGNTDLYKIKQEKDAIYANPEATMEDYTRAGELEEKFGELGG", "aa_hexdigest": "f387d22bab52c37a23d576cc4bd3c611", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002614D0AA", "query_cov": 1.0, "subject_cov": 0.9343065693430657, "identity": 0.57, "score": 138.0, "evalue": 9.1e-40, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002614D0AA" }, "pscc": { "uniref50_id": "UniRef50_UPI002614D0AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_571", "locus": "GCA000148625_00539" }, { "type": "cds", "sequence": "contig_1", "start": 307476, "stop": 308099, "strand": "-", "frame": 3, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGCAAGTAATTGAAATCAAAAGGACTGTTTCGATGCCACTAACCAATCAAAATATTTATTTTCTTAAAATACCTTATGTTACAAATCTAATTACTTTAAAAAACTTTTTCTTTTCAATGACTAAAAAACAAAATGCCATTAAAGAAGTTATGAGATATTTTGACAGCTTAATTAGTGAATTAGAAAATACTAATACAATTATTATTCCTGATGAATTTATAAATAGCAAAATATCAGATTATACTCATCAATATTTAGAAAAAGTAAATCCAAATAATCGAGCAGCTATTGAAAAAAGTATTCAAGAAAAAGATCAATATGGTAAACAAATAATAGATAATGCGATTAATAGTGCTAGAAGAGAAATTGTTGAAGACATTGTTAAAAAATTAGTTTACACATTAGAAAAAATAAAAGTCCATGACACAGAAGTAAATCAAGAATTAAATCTAAGCAAAAATATTTTATTTCAATCAAACAACTATTACAATAGAAATGAAAACAAAAATGTTAACACTGTAAAAGAAGATTTAATTAATATAAAAATAGCTTTAAAACTTTTACAACTAAATGCACCTCAATTATTTTTAGTAATTGCTAGAGATGCAGCTATTGCAGTATAG", "aa": "MQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSKNILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV", "aa_hexdigest": "f2c3d184c023391bde0e6e435e225cb8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 307476, "stop": 308399, "observations": { "mutation": [ 308105 ], "stop": [ 308105 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4928571428571429, "identity": 0.995, "score": 382.0, "evalue": 1.39e-130, "gene_start": 1, "gene_end": 207, "reference_start": 214, "reference_end": 420, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.993485342019544, "score": 600.0, "evalue": 1.07e-218, "description": "Internal stop codon at 308105. Nonsense mutation around 308105.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "IGIGPGHMMC_572", "locus": "GCA000148625_00540" }, { "type": "cds", "sequence": "contig_1", "start": 308103, "stop": 308708, "strand": "-", "frame": 3, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGATCAATTATTTATTAAAAAGAGAAAATATTAAAATGAATAAAGATGAAATTGAGGCAATGATAAATAATACTGTTAGAGATTTAAAAATTGACAGAACTAAAATTGATTCAATGTCTTTATTAGAAAAATCTTTTGATTATAAATGCAAAAAAATATATAACTTAATTTCAAATGAAATTTTAAAAAGTGATGCTTTAGCCTATATTGCTAGTCCAGCAATTAGAAAAGATATTATTTCAAATAACACTATTAGTGCGGAAATTGTGGTTGCTTATTATCCTTTTGAAGAGCTTAAAAAAATTAACTATAAAAATACTCCAGTAAATTATATTTACAACTTTTTTGATGAAAATTCAATTGGTGCAATTTTTGAAAATACAATTAAAAATTACACATTTAAAAATTCAGTAAATGATTTTATTAAACAAAACGACTTAGTTAAGTTAAACATTAAATTATTTCAAAATAATATCTTAATGAAAGATGATAAAAATGTTCTTATAATAGCTAACCACACAAACAACTTTACTATTAATAATTCAATTCTTAATCATAGATATGGTGAAATTTTTAGCATCAATGATCCTTTAGGTAGAAAATGA", "aa": "MINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRK", "aa_hexdigest": "0a93f1f061d39da5c95b12f4aa85f321", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 307800, "stop": 308738, "observations": { "mutation": [ 308105, 308723 ], "stop": [ 308105, 308723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4785714285714286, "identity": 1.0, "score": 389.0, "evalue": 2.86e-133, "gene_start": 1, "gene_end": 201, "reference_start": 11, "reference_end": 211, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.9903846153846154, "score": 610.0, "evalue": 6.6e-223, "description": "Internal stop codon at 308105, 308723. Nonsense mutation around 308105, 308723.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "IGIGPGHMMC_573", "locus": "GCA000148625_00541" }, { "type": "tRNA", "sequence": "contig_1", "start": 309006, "stop": 309080, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 80.8, "nt": "GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 309038, 309040 ], "id": "IGIGPGHMMC_8", "locus": "GCA000148625_00542" }, { "type": "tRNA", "sequence": "contig_1", "start": 309084, "stop": 309159, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.4, "nt": "GGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 309117, 309119 ], "id": "IGIGPGHMMC_9", "locus": "GCA000148625_00543" }, { "type": "tRNA", "sequence": "contig_1", "start": 309164, "stop": 309239, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 79.7, "nt": "GGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 309197, 309199 ], "id": "IGIGPGHMMC_10", "locus": "GCA000148625_00544" }, { "type": "tRNA", "sequence": "contig_1", "start": 309241, "stop": 309316, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 91.8, "nt": "GCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 309274, 309276 ], "id": "IGIGPGHMMC_11", "locus": "GCA000148625_00545" }, { "type": "tRNA", "sequence": "contig_1", "start": 309408, "stop": 309492, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 59.4, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 309436, 309438 ], "id": "IGIGPGHMMC_12", "locus": "GCA000148625_00546" }, { "type": "cds", "sequence": "contig_1", "start": 309654, "stop": 309791, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGATAATTAATTTTAAAATGTTTGAAAATGCAGCAATTAGACACAACTTGAAAATTTTGCAAGTTGATTATTTTGAAAAAAATATAACAATTGATATTTTTTGTGGCAACAATTCTTATATTAAATGA", "aa": "MKNKIINFKMFENAAIRHNLKILQVDYFEKNITIDIFCGNNSYIK", "aa_hexdigest": "babf2593f658da377b49c0a53648ce13", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.2961, "isoelectric_point": 9.302159309387207 }, "id": "IGIGPGHMMC_574", "locus": "GCA000148625_00547" }, { "type": "cds", "sequence": "contig_1", "start": 309940, "stop": 310104, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01330.27" ], "nt": "ATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGA", "aa": "MVLYRIGEIVYKNNQNLIFESQGVGYSIIFPDHARVEAKQKMKLYLYEIRNDYT", "aa_hexdigest": "48038776229e059bd268cfe29ed1aa7b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01330.27", "name": "RuvA_N", "length": 51, "aa_cov": 0.9074074074074074, "hmm_cov": 1.0, "evalue": 7.378759393935326e-08, "score": 28.46644401550293, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 6473.391900000002, "isoelectric_point": 8.005506706237792 }, "id": "IGIGPGHMMC_575", "locus": "GCA000148625_00548" }, { "type": "cds", "sequence": "contig_1", "start": 310405, "stop": 310542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAG", "aa": "MLGFKAAQIDSALTKVELNQDVESMIEEAIKFISQSGAQNEQLNA", "aa_hexdigest": "7e7e499e2935b65702a950444eae9e99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 35, "aa_cov": 0.7333333333333333, "hmm_cov": 1.0, "evalue": 2.5360522419180546e-08, "score": 30.333208084106445, "start": 2, "stop": 34 } ], "seq_stats": { "molecular_weight": 4911.4763, "isoelectric_point": 4.250954246520997 }, "id": "IGIGPGHMMC_576", "locus": "GCA000148625_00549" }, { "type": "cds", "sequence": "contig_1", "start": 310535, "stop": 310804, "strand": "+", "frame": 2, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244E5E3C", "UniRef:UniRef90_UPI0002D51167" ], "nt": "ATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGAGGTGCTGTAA", "aa": "MLRPANFFEFVGQEKLIYTLKTMIEGSRYRKEPLDHILFYGPPGTGKTSLASILANELDTKIHYLQGALLEKKSDILTVFANVKENEVL", "aa_hexdigest": "1306c8b664f7ad8756f0ec6c732c8140", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D51167", "query_cov": 1.0, "subject_cov": 0.956989247311828, "identity": 1.0, "score": 181.0, "evalue": 8.46e-58, "valid": true, "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI00244E5E3C" }, "pscc": { "uniref50_id": "UniRef50_UPI00244E5E3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244E5E3C" ], "product": "AAA family ATPase" }, "genes": [], "id": "IGIGPGHMMC_577", "locus": "GCA000148625_00550" }, { "type": "cds", "sequence": "contig_1", "start": 310899, "stop": 311105, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTTTTTCAAAACTATGGATTAAATTATGACAAATTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTATAAAATTCCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANYKIPETKTESLFKKISSKSLFEKM", "aa_hexdigest": "a9fa74e5e4f7a5d33cacb2c6b702f010", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 310899, "stop": 311208, "observations": { "insertion": [ 311178 ], "mutation": [ 311103 ], "stop": [ 311103 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.971, "score": 125.0, "evalue": 4.27e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9411764705882353, "score": 181.0, "evalue": 1.33e-60, "description": "Internal stop codon at 311103. Frameshift due to insertion around 311178. Nonsense mutation around 311103.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8026.149699999999, "isoelectric_point": 9.363984489440917 }, "id": "IGIGPGHMMC_578", "locus": "GCA000148625_00551" }, { "type": "cds", "sequence": "contig_1", "start": 311169, "stop": 311393, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATCAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGCAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDQLVSMNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "989476376e6950332198b2b96c0fbd3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8703.398399999991, "isoelectric_point": 10.220898246765138 }, "id": "IGIGPGHMMC_579", "locus": "GCA000148625_00552" }, { "type": "cds", "sequence": "contig_1", "start": 311505, "stop": 311777, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTAATAATAAAGTTTTAATTAACAATAAGTTTTTAAATTTATCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTNNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "2ad996294a11539a5db3f8419765547e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10697.456499999998, "isoelectric_point": 9.855878257751463 }, "id": "IGIGPGHMMC_580", "locus": "GCA000148625_00553" }, { "type": "cds", "sequence": "contig_1", "start": 312616, "stop": 313116, "strand": "+", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACACAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATTAATAAGCCTTCTAATTTTGTTTCACTAAGCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGCGCTAAATGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACTAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETQLKRALTNDATSKLIKEIINKPSNFVSLSNPFDVKTKIKQSFLKHQERIYEKFIQYCAKCLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "5b52e27ba7fdd5661ef452fc92677ef4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 312616, "stop": 313419, "observations": { "mutation": [ 313114, 313237, 313363 ], "stop": [ 313114, 313237, 313363 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9279999999999999, "score": 308.0, "evalue": 3.42e-104, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9250936329588015, "score": 494.0, "evalue": 8.86e-180, "description": "Internal stop codon at 313114, 313237, 313363. Nonsense mutation around 313114, 313237, 313363.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "IGIGPGHMMC_581", "locus": "GCA000148625_00554" }, { "type": "cds", "sequence": "contig_1", "start": 314608, "stop": 314736, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGTAAAAATATTGTTATATATTTTAAAGACAAGGAAGAGTATAAAACTTTTAAGCAAGTATGGAAACAAATCCCAAACAAAAGCGAATATATTAAACAAATTCTCAAAGAATTCAAAAAATAA", "aa": "MKRKNIVIYFKDKEEYKTFKQVWKQIPNKSEYIKQILKEFKK", "aa_hexdigest": "05dcb36654f62ce7fabb2c76f913bd1e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5367.355699999999, "isoelectric_point": 9.941105461120607 }, "id": "IGIGPGHMMC_582", "locus": "GCA000148625_00555" }, { "type": "cds", "sequence": "contig_1", "start": 315255, "stop": 315725, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354528.1", "SO:0001217", "UniParc:UPI0001E33097", "UniRef:UniRef100_UPI0001E33097", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTATCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAATATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAAAAATTTACTTGAACTAAATATATATTATGAATTACTTAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESIIKLIDSKTIINFDIAKLQNINKYFGFGISDINLLKINQVLFDYELLKTLRLEKNLLELNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEEKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "885e4bc60f8592ed9865b47a876efa7c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33097", "ncbi_nrp_id": "WP_013354528.1", "uniref100_id": "UniRef100_UPI0001E33097", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33097", "RefSeq:WP_013354528.1", "UniRef:UniRef100_UPI0001E33097" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33097", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18935.841799999995, "isoelectric_point": 8.911029624938966 }, "id": "IGIGPGHMMC_583", "locus": "GCA000148625_00556" }, { "type": "cds", "sequence": "contig_1", "start": 315739, "stop": 315903, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01330.27" ], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAAATAATGTTGGATACTATGTTATGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTATTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESNNVGYYVMFPDNKRIKLNQELKMYLYEIKNDFT", "aa_hexdigest": "a3c382ae05ee79a9a43caeaa675937f5", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01330.27", "name": "RuvA_N", "length": 51, "aa_cov": 0.9074074074074074, "hmm_cov": 1.0, "evalue": 2.475576452031578e-07, "score": 26.782733917236328, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 6586.6554000000015, "isoelectric_point": 9.435415458679199 }, "id": "IGIGPGHMMC_584", "locus": "GCA000148625_00557" }, { "type": "cds", "sequence": "contig_1", "start": 315855, "stop": 316046, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAACCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAACTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAATACATTTTTAATTCTTGA", "aa": "MLPSLIKFIALSNPTFKTLNATLGPMPFKLTKSANNFLSSNSLKPYKDQVKSFLISYKYIFNS", "aa_hexdigest": "f7b5e09c891e22a6d17cadae6a7fc1b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7120.332400000003, "isoelectric_point": 10.079325675964352 }, "id": "IGIGPGHMMC_585", "locus": "GCA000148625_00558" }, { "type": "cds", "sequence": "contig_1", "start": 316158, "stop": 316340, "strand": "+", "frame": 3, "gene": "ruvA", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354530.1", "SO:0001217", "UniParc:UPI0001E33099", "UniRef:UniRef100_UPI0001E33099", "UniRef:UniRef50_C4XEG5", "UniRef:UniRef90_C4XEG5" ], "nt": "ATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGAAGCAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCAATAGAAGAAGCAATTAAATTAATGCCCGATAAACTAAAAACACTATAA", "aa": "MFENYVTTYKSSKEINETLKMLGFKDSLIDEAVRKINLNQDIEFSIEEAIKLMPDKLKTL", "aa_hexdigest": "ae1ac31aae8cf1ac520d377692d7a3f4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33099", "ncbi_nrp_id": "WP_013354530.1", "uniref100_id": "UniRef100_UPI0001E33099", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33099", "RefSeq:WP_013354530.1", "UniRef:UniRef100_UPI0001E33099" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33099", "uniref90_id": "UniRef90_C4XEG5" }, "psc": { "uniref90_id": "UniRef90_C4XEG5", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEG5" }, "pscc": { "uniref50_id": "UniRef50_C4XEG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG5" ], "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein" }, "genes": [ "ruvA" ], "id": "IGIGPGHMMC_586", "locus": "GCA000148625_00559" }, { "type": "cds", "sequence": "contig_1", "start": 316359, "stop": 316799, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTACATCCTATAACTAGTAATAATATTATTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTCTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCTAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLHPITSNNIIDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRLKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "91bd263e811d26539df0ec5a95d8d0b5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 0.986, "score": 278.0, "evalue": 1.69e-94, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "IGIGPGHMMC_587", "locus": "GCA000148625_00560" }, { "type": "cds", "sequence": "contig_1", "start": 317050, "stop": 317523, "strand": "+", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAACTCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCGATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTGCCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACCTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAATCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGTCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGTGTTAATTGA", "aa": "MRNSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNLSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLSRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGVN", "aa_hexdigest": "1e643d05ed7f5542887b9192d5500dc4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 317050, "stop": 317721, "observations": { "mutation": [ 317521 ], "stop": [ 317521 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32E9F", "query_cov": 1.0, "subject_cov": 0.7040358744394619, "identity": 0.981, "score": 304.0, "evalue": 1.74e-103, "gene_start": 1, "gene_end": 157, "reference_start": 1, "reference_end": 157, "reference_sequence": "MRNSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISLSTNYVKKYSEINKLTKPNVDIFDN" }, "paralog": false, "identity": 0.9641255605381166, "score": 429.0, "evalue": 4.42e-155, "description": "Internal stop codon at 317521. Nonsense mutation around 317521.", "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32E9F", "UniRef:UniRef50_UPI0001E32E9F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" } }, "id": "IGIGPGHMMC_588", "locus": "GCA000148625_00561" }, { "type": "cds", "sequence": "contig_1", "start": 317575, "stop": 317745, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGACCTATGAAAAAGAATGGCACAACAATTTATTTTTCATTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAATTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGA", "aa": "MHGPMKKNGTTIYFSLNMPNTISMSTNYVKKYSEINKLIKPNVDIFDNLIKRLKIK", "aa_hexdigest": "ec81a4166611dee24c4bc6022bd61a99", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.7178, "isoelectric_point": 9.998740196228027 }, "id": "IGIGPGHMMC_589", "locus": "GCA000148625_00562" }, { "type": "cds", "sequence": "contig_1", "start": 318264, "stop": 318395, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATTTTATCAACCATACCATTAAAAATTAAAGGTCGAGCTTTTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTACAGCAGAAGATGATGAATGA", "aa": "MNTPNNKINILSTIPLKIKGRAFIEDYLKLKVNSILFTAEDDE", "aa_hexdigest": "77642c8f526b6aaa6b25cc0a2ebbd9a8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4921.6649, "isoelectric_point": 6.142328453063963 }, "id": "IGIGPGHMMC_590", "locus": "GCA000148625_00563" }, { "type": "cds", "sequence": "contig_1", "start": 318396, "stop": 318764, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_UPI0001E3309C" ], "nt": "TTGTTTTTTCTTGCATGTTATCCCAAAAATGCAAAGGTAAAAAATAATAAAAAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGGTGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATTAGCGCCAGATGAAAGTTATCCTTTTTTGAATTTTAAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACCAATGGTGAATTATCTAAAGCACCGAAAATAGTAATAATAAACAAAAAAGGCGACAAGTTTTTAGATATAAAGAATAAGGAGTTAATAAAAAATGACATACAATAA", "aa": "MFFLACYPKNAKVKNNKKNLIDIRNNQVNSKGELDDVKEISSYIDLSHIFKIHVKNLKDFVKLAPDESYPFLNFKKQLEYVEKITSLTNGELSKAPKIVIINKKGDKFLDIKNKELIKNDIQ", "aa_hexdigest": "ed7362982b16c3f5b7fb40a6794091a3", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 318264, "stop": 318764, "observations": { "mutation": [ 318393 ], "stop": [ 318393 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E3309C", "query_cov": 1.0, "subject_cov": 0.7349397590361446, "identity": 0.992, "score": 236.0, "evalue": 2.75e-78, "gene_start": 1, "gene_end": 122, "reference_start": 45, "reference_end": 166, "reference_sequence": "MNTPNNKINILSTIPLKIKGRAFIEDYLKLKVNSILFTAEDDEWLFFLACYPKNAKVKNNKKNLIDIRNNQVNSKGELDDVKEISSYIDLSHIFKIHVKNLKDFVKLAPDESYPFLNFKKQLEYVEKITSLTNGELSKAPKIVIINKKGDKFLDIKNKELIKNDIQ" }, "paralog": false, "identity": 0.9939759036144579, "score": 326.0, "evalue": 4.58e-116, "description": "Internal stop codon at 318393. Nonsense mutation around 318393.", "psc": { "uniref90_id": "UniRef90_UPI0001E3309C", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E3309C", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14171.356699999977, "isoelectric_point": 9.406662559509279 }, "id": "IGIGPGHMMC_591", "locus": "GCA000148625_00564" }, { "type": "cds", "sequence": "contig_1", "start": 318751, "stop": 319110, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTAATTATGAGAAAACCGAATAAATTTTTATCTAAATCTAAACCTTATAACAGTAAAGAAATTATAAATTTAGAATATTACAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAAAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKPYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFKKNGDTSCLTVL", "aa_hexdigest": "74fb4170e67ad5a1d27f96f930db0e23", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14301.649199999978, "isoelectric_point": 9.664471626281738 }, "id": "IGIGPGHMMC_592", "locus": "GCA000148625_00565" }, { "type": "cds", "sequence": "contig_1", "start": 319384, "stop": 319602, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "IGIGPGHMMC_593", "locus": "GCA000148625_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319637, "stop": 320083, "strand": "+", "frame": 2, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTACTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTTTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCTCCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "4164854a7130acf16c96c160bbf5ab07", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 319598, "stop": 320227, "observations": { "mutation": [ 320081 ], "stop": [ 320081 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 8.86e-94, "description": "Internal stop codon at 320081. Nonsense mutation around 320081.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "IGIGPGHMMC_594", "locus": "GCA000148625_00567" }, { "type": "cds", "sequence": "contig_1", "start": 320126, "stop": 320227, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 3.899520131836104e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "IGIGPGHMMC_595", "locus": "GCA000148625_00568" }, { "type": "cds", "sequence": "contig_1", "start": 320233, "stop": 320373, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAGAAGAAAATAATACAATGCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSEENNTMPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "4c9fa38b941faff729b4441fe9f4c8fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.020600000001, "isoelectric_point": 5.1057558059692365 }, "id": "IGIGPGHMMC_596", "locus": "GCA000148625_00569" }, { "type": "cds", "sequence": "contig_1", "start": 321921, "stop": 322010, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAGCAGATTAAGATTAGGTTTAATAACAACATTGGCTAGTGGAACAATGGCTTTGCCATTAGTTTCATCTAGTTGTTGA", "aa": "MKKKSRLRLGLITTLASGTMALPLVSSSC", "aa_hexdigest": "bbf0ccf1d0fcb9f9d9dabe838d988e7e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3063.7445, "isoelectric_point": 11.105404472351072 }, "id": "IGIGPGHMMC_597", "locus": "GCA000148625_00570" }, { "type": "cds", "sequence": "contig_1", "start": 322749, "stop": 323027, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTGATGATGCTTGTAAAGAACTTCATCAAGCAACAAAAATAGCATCTGCTTTAGGAGCATTTGAAATAATATGCGATCCAACAAAAACAGTGATTTATCAGTTAGATTTATATAAAAGTTTAATTGCTTTTTTCAATAATATTTATGCACTTAAACTTGATAAGACTAAACCGATTGAAGAACAATTAAAAACGGAAGAAATTAAAAATTCGCTACGTGATGCAATAAATAATGCAACAACATCATTGCAAGAAATGATGCCAGCTGGTTGA", "aa": "MVIDDACKELHQATKIASALGAFEIICDPTKTVIYQLDLYKSLIAFFNNIYALKLDKTKPIEEQLKTEEIKNSLRDAINNATTSLQEMMPAG", "aa_hexdigest": "d5eb499437dc44821822f71c6200338b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10306.83569999999, "isoelectric_point": 5.017257499694824 }, "id": "IGIGPGHMMC_598", "locus": "GCA000148625_00571" }, { "type": "cds", "sequence": "contig_1", "start": 323109, "stop": 323279, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGATAAAAAATACCCAACAGCCAACTATTATCTATTTAATGCTGATAATCCTGATTGGGACCACTCACAAGCTAGGTTTGTTACCGATGTCTGCTACTATCTCAAAGAATTCTTATTACCACTTGCTGCCTTAATCAAGCTAGAAATTAGTGATGGATTCTGA", "aa": "MQYDKKYPTANYYLFNADNPDWDHSQARFVTDVCYYLKEFLLPLAALIKLEISDGF", "aa_hexdigest": "c618890f085b2434c6ce8295022669b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6656.460499999997, "isoelectric_point": 4.65075969696045 }, "id": "IGIGPGHMMC_599", "locus": "GCA000148625_00572" }, { "type": "cds", "sequence": "contig_1", "start": 323651, "stop": 323986, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_041593932.1", "SO:0001217", "UniParc:UPI0002F783AE", "UniRef:UniRef100_UPI0002F783AE", "UniRef:UniRef50_UPI0002F0BBCD", "UniRef:UniRef90_UPI0002F0BBCD" ], "nt": "ATGAATATTACTACAACAATGTTTCGCAATGTAGATACAAGTACAACAGCTGAAACAACATTTAAAAATATACAACATGCATTGATAGGCCAAGCTATTACTTGGTCAAGTATTATACTTGGTTCCTTATTTGCCTTAGCTGTTATTATTACTATTATAATTGTTAGTCTTAAGGCAACTCTTGGCAAAAGAGATGATTCTTACCAAGAAAAATTAAAAGTTGTATTAAAAAGCTGTTTAATTGCAACTGCTGTACTTCTTGCGCTTCTTATTCTAACACCATCAATTTTAAAAATAATTCAAACTAATTATGGTGGCGAAGCACTTAAAGGATAA", "aa": "MNITTTMFRNVDTSTTAETTFKNIQHALIGQAITWSSIILGSLFALAVIITIIIVSLKATLGKRDDSYQEKLKVVLKSCLIATAVLLALLILTPSILKIIQTNYGGEALKG", "aa_hexdigest": "15a148bfe65195c1c09f66df1d441af3", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002F783AE", "ncbi_nrp_id": "WP_041593932.1", "uniref100_id": "UniRef100_UPI0002F783AE", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F783AE", "RefSeq:WP_041593932.1", "UniRef:UniRef100_UPI0002F783AE" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F783AE", "uniref90_id": "UniRef90_UPI0002F0BBCD" }, "psc": { "uniref90_id": "UniRef90_UPI0002F0BBCD", "uniref50_id": "UniRef50_UPI0002F0BBCD" }, "pscc": { "uniref50_id": "UniRef50_UPI0002F0BBCD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F0BBCD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11992.152599999983, "isoelectric_point": 9.463846015930176 }, "id": "IGIGPGHMMC_600", "locus": "GCA000148625_00573" }, { "type": "cds", "sequence": "contig_1", "start": 324083, "stop": 324838, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0396 family ICE element transmembrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FACEB8", "UniRef:UniRef90_UPI0002FACEB8" ], "nt": "TTGATTAACTTTTTATCATTTGATTTTATATCATTATTAATTTTCGGTAAATCAATAGATGATATTAAATCAATAGATGATATAAAAAGCATACCAATGGCTTTTGCAGGGCTAGCACTTTTTGCTTTTGTTATGATTGCAATAATCCTTGGTATTGCTTGTTTTAAAGTCTTTTTTGGTGGTGGCGGAGAAGACAAAATGCAAAAGATGAAGCAGAGTTTCAAAGCGGCATTACCACATATGGCTTTTATAATCTTAATTCCAATTGTCGTTTGGGTCCTTGCATTTTTTGCAAAATTTGCATTTAATATGCTAAATCTTGCTTTAGGGGTAGACCCAGCTGCAGGTTTAGGGGAAATAATTTATAAAGCGATAAATCAACCAGAGGCAAGATTTCCAAATCCAACAGAACCAACATATGGCCAATACTCTGCTTCACAAAATGAACTTAATAGAAATAGCGTTGTATTAATGATAAATGCATTAGTACTGATTTGGTCAATGATAGTTATTGCAAATATTGCTATAAATATATCAATCAACTCATATCAAGCATTCTTTTTATTTATACAATCGCCATTAATAGCAGTAGTCTCAATTGAAGATGGTGGCAAGAAGCTTAATATATGGAAGCAACAATTTGTGTCCAAGATTCTTTCCGTTATGGTCTTGAATTTATCATTGTCAGTACTGGTTATGTTTTTTTCAGCTGCCTCAAAAGTTATAATGCCTATGGCTAACGCAACTAAAGTATGA", "aa": "MINFLSFDFISLLIFGKSIDDIKSIDDIKSIPMAFAGLALFAFVMIAIILGIACFKVFFGGGGEDKMQKMKQSFKAALPHMAFIILIPIVVWVLAFFAKFAFNMLNLALGVDPAAGLGEIIYKAINQPEARFPNPTEPTYGQYSASQNELNRNSVVLMINALVLIWSMIVIANIAINISINSYQAFFLFIQSPLIAVVSIEDGGKKLNIWKQQFVSKILSVMVLNLSLSVLVMFFSAASKVIMPMANATKV", "aa_hexdigest": "1965562e6e36d48de11b6fe4ae25b1be", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 323993, "stop": 325141, "observations": { "mutation": [ 324032, 324836, 324842 ], "stop": [ 324032, 324836, 324842 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002FACEB8", "query_cov": 1.0, "subject_cov": 0.5295358649789029, "identity": 0.996, "score": 445.0, "evalue": 6.25e-154, "gene_start": 1, "gene_end": 251, "reference_start": 31, "reference_end": 281, "reference_sequence": "MLKRIGFFFFKIGWFPFVAFPFTIINSLGKLINFLSFDFISLLIFGKSIDDIKSIDDIKSIPMAFAGLALFAFVMIAIILGIACFKVFFGGGGEDKMQKMKQSFKAALPHMAFIILIPIVVWVLAFFAKFAFNMLNLALGVDPAAGLGEIIYKAINQPEARFPNPTEPTYGQYSASQNELNRNSVVLMINALVLIWSMIVIANIAINISINSYQAFFLFIQSPLIAVVSIEDGGKKLNIWKQQFVSKILSVMVLNLSLSVLVMFFSAASKVIMPMANATKVWPWLIALIVFVAALYSTKSLLKIVCSIFGVEMTNFVSNAVNKVKSLHPVQKLKNKLKKKNAGADGGGDSADNESEGSSDGKSNDENANTSDATAYSVKDETNKKAISTEKIPPADIQKTKENIEGVAGIRGIPDLSIYKNGDIQQKLSDISLSSKDIVKNLAEMKKASQELVKHQEIDNYKDSIKETKKHESK" }, "paralog": false, "identity": 0.9921465968586387, "score": 647.0, "evalue": 5.78e-236, "description": "Internal stop codon at 324032, 324836, 324842. Nonsense mutation around 324032, 324836, 324842.", "psc": { "uniref90_id": "UniRef90_UPI0002FACEB8", "product": "Mbov_0396 family ICE element transmembrane protein", "uniref50_id": "UniRef50_UPI0002FACEB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002FACEB8", "UniRef:UniRef50_UPI0002FACEB8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FACEB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FACEB8" ], "product": "Mbov_0396 family ICE element transmembrane protein" } }, "id": "IGIGPGHMMC_601", "locus": "GCA000148625_00574" }, { "type": "cds", "sequence": "contig_1", "start": 324929, "stop": 325417, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACTTTGTTTCAAACGCAGTTAATAAAGTTAAAAGCCTACACCCAGTACAAAAATTGAAGAACAAACTCAAAAAGAAAAATGCTGGTGCTGATGGAGGAGGAGATTCTGCTGATAATGAAAGTGAAGGTTCCTCTGATGGAAAAAGCAATGATGAAAATGCAAATACGAGTGATGCAACAGCTTATTCTGTTAAAGACGAGACCAATAAGAAAGCAATAAGCACTGAGAAAATTCCGCCAGCAGATATCCAGAAAACCAAAGAAAATATCGAAGGCGTAGCAGGAATAAGGGGAATACCTGATCTTTCAATTTATAAAAATGGAGATATTCAACAAAAATTAAGCGATATTTCTCTTTCATCAAAAGATATAGTGAAAAATCTTGCTGAAATGAAAAAAGCATCCCAAGAACTTGTAAAACATCAAGAAATAGATAACTATAAAGATAGCATAAAGGAGACAAAAAAACATGAATCAAAATAA", "aa": "MTNFVSNAVNKVKSLHPVQKLKNKLKKKNAGADGGGDSADNESEGSSDGKSNDENANTSDATAYSVKDETNKKAISTEKIPPADIQKTKENIEGVAGIRGIPDLSIYKNGDIQQKLSDISLSSKDIVKNLAEMKKASQELVKHQEIDNYKDSIKETKKHESK", "aa_hexdigest": "787cf80ad440593afa842d770435d206", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17615.386899999998, "isoelectric_point": 8.553616905212404 }, "id": "IGIGPGHMMC_602", "locus": "GCA000148625_00575" }, { "type": "cds", "sequence": "contig_1", "start": 325404, "stop": 325538, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAAATAAGAAAATAAGCTCATTAAAGCTTAATATTGTAAAGAATTTAAACCTTTATGATATTTTGTTGTTATTTGCAATAATAGGCTTTTCAGCAGCATTAGGATTTGCTAATAAACTTAACACTTGA", "aa": "MNQNKKISSLKLNIVKNLNLYDILLLFAIIGFSAALGFANKLNT", "aa_hexdigest": "685bbec0df066b04fb511772ff825a23", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4880.789600000001, "isoelectric_point": 10.000287437438963 }, "id": "IGIGPGHMMC_603", "locus": "GCA000148625_00576" }, { "type": "cds", "sequence": "contig_1", "start": 325584, "stop": 326471, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAATACCAAGTAAAAAACACAATTGCAGGCTCTATACGTTAATAGTTAGAATATTTAAATATAAATCCTCTACGAAGAAATATAAAAAGAATACACCAAATGATACAAAACTACTTGTACCATTTAAGAGAATAATTGAAAGTAAATATGTTGAAACATTATCATCAGCTGGAACAAGTATGTTTACTGTGTTTAAATTTAATGGTTTTAATATTTACTCAAACAATAGAGATGATAAATACGCATTTTTTAATCAATTAATTAATGTATTTAACTCAATTGAAACCAAATTCACATTTGTTAAAAAGCCAGCTTCTATGAATATAGAAGATAACTTAAAATACAATGTTGATAAGTATTTTGAAAGCAAAATGTTACAAAGCAACGAACTTGTTCAAAACCATTTTATTTACAATACACATGACTTTGAACAACTTAAAAAAATAGAAAACATTGATATTTATTATCTTGTTATTTATGGTAAGAATATAAATGAAATTAAACAACTAGAAAATGAGTTTATCAAGTCATTTGAAAATACCAAAATACCTTTATCAAATGTCACCGGCTATGAACTATTAGACTTTACAAATATGATTTACAATGTATCATTATCACAAAAACAAAAACAAAATTTCTTTGACGAAACACTTGATGAAGAGCAAACTATGATATTAGATGCTAAAATGAAAGGCAAAGAACTTTCAGACAAGGAATTAAAGAAATTACGTAAAGATAAAGAATATAAGCTTATGAACTTGCTTGCACCTGAAACAATAGAGTTCGGTTCAAAGCATATAACAATTAATAAAGGAGAAAATGAAAAATATTTATCATTTCAAACCTTATATGATTTTAGTTCTTTAAAAATTAATGAAGGTTGA", "aa": "MIIPSKKHNCRLYTLIVRIFKYKSSTKKYKKNTPNDTKLLVPFKRIIESKYVETLSSAGTSMFTVFKFNGFNIYSNNRDDKYAFFNQLINVFNSIETKFTFVKKPASMNIEDNLKYNVDKYFESKMLQSNELVQNHFIYNTHDFEQLKKIENIDIYYLVIYGKNINEIKQLENEFIKSFENTKIPLSNVTGYELLDFTNMIYNVSLSQKQKQNFFDETLDEEQTMILDAKMKGKELSDKELKKLRKDKEYKLMNLLAPETIEFGSKHITINKGENEKYLSFQTLYDFSSLKINEG", "aa_hexdigest": "2c2a221e1949d75638423ee0969f9ff9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34956.719699999994, "isoelectric_point": 9.027910804748537 }, "id": "IGIGPGHMMC_604", "locus": "GCA000148625_00577" }, { "type": "cds", "sequence": "contig_1", "start": 326562, "stop": 326897, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAAATAATGCTAATGCAGACACAAAAAGTAATTTTAAAATTCAAAAAATTAGAGTTGAACAAGAAGTACTGGACGAACTAGTTAATTTTATTAACGTTCAAAAAATGAACTTATTTGATTCAAATTTAATTTTTGTTAATCAAAGTAAAGATTTGAAAACATTACACGAAATTGAAGTTCGGAATGGAATAAACGCTAAAAAAGCATTTGCCAATGTTTATTTAAATCACTATTTACAAAAAGAAGCTTTACAAAACTCAATATTTATCAAAGAAGACTTTTTGAGAAATGGCATTGAAATGAGCTCGCGTAATATTGTTCATGGTTGA", "aa": "MQLNNANADTKSNFKIQKIRVEQEVLDELVNFINVQKMNLFDSNLIFVNQSKDLKTLHEIEVRNGINAKKAFANVYLNHYLQKEALQNSIFIKEDFLRNGIEMSSRNIVHG", "aa_hexdigest": "7b34bd1891e7c9523cf221dff3146fed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12908.595499999985, "isoelectric_point": 8.005506706237792 }, "id": "IGIGPGHMMC_605", "locus": "GCA000148625_00578" }, { "type": "cds", "sequence": "contig_1", "start": 327171, "stop": 328004, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGCAAAGGACTTGAAACCAAAATAAATCCTTTACAAGTTAACTTTGAAAGTGAAAAGGATAATGTTGCTGTTACAAACGATATGTTAATAACTAACCATATTAAGAAAACTAAAGAATTCTTTTCATTATTGTATCCTGACTTAGATGAAAAGGATTTAAGAGTTGTGACTTTCACACTATCAGAGTTTTATGCTAAGCAAAAATTCAAACCTATTGAAAAGATGAAAAATAATGAATTCCCAACAATGATTGATTATGTTAATTTCTTAGGTAAATATAAATTTAGCGATAAGAAGACAGAGTTATATTACAAAGATTCAATTAATAAACTATTTATAATCTTTAATCAAGATTTCTTAAATAATGGAATATTTAGTGCATTATATACTGGACATACAAATATTGATTTTGATAATTCATTTACTGTTATTGATACATATAACTTAATTAATAATATTTCTTCAAACGAAGGACAAGCATCATTATTCTTGATTCTTTCACACGTTAGAAATAGAATATCAAACAACTTCTTTATTAACAAAACTAAGAAGATTTGTTTAATTGTTGATGAAGCACATAGATTTATTAATGCTAAGAATATGTCTTCATTAGAATTCTTATTTGACACAGTTAAAACAATTCGTAAATACAACGGTTCTGTTATTTTAACTACACAAAACCCAAGTGACTTTGAATTAAGTGAAGACGTTATGAACCTTACAAAAGGTATGGTTGAAAATATACAATATACACTTATTTTAAGACAAAATCAAAATGGTGTTAATGCTATTAGTCAAATGTATAAATCTAAAAATGAGTTAACTTATTGA", "aa": "MGKGLETKINPLQVNFESEKDNVAVTNDMLITNHIKKTKEFFSLLYPDLDEKDLRVVTFTLSEFYAKQKFKPIEKMKNNEFPTMIDYVNFLGKYKFSDKKTELYYKDSINKLFIIFNQDFLNNGIFSALYTGHTNIDFDNSFTVIDTYNLINNISSNEGQASLFLILSHVRNRISNNFFINKTKKICLIVDEAHRFINAKNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSEDVMNLTKGMVENIQYTLILRQNQNGVNAISQMYKSKNELTY", "aa_hexdigest": "7803d757b450daf7b302c2113a2ea6cb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 51, "aa_cov": 0.17689530685920576, "hmm_cov": 1.0, "evalue": 1.5075197223668948e-06, "score": 23.81523323059082, "start": 183, "stop": 231 } ], "seq_stats": { "molecular_weight": 32243.46380000003, "isoelectric_point": 7.710516166687013 }, "id": "IGIGPGHMMC_606", "locus": "GCA000148625_00579" }, { "type": "cds", "sequence": "contig_1", "start": 328195, "stop": 328344, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACACAAAAAAATATGTCTTTTAGCAGTGCTTAGTGGGTTTGTACCATTAAGTACTATTTTCATTTCAAACTCTGTTAATACACAAAATAATAATGTTAATAAAGAAACTAATATAACAAAGAAAATCACTGTTAATTATTGA", "aa": "MKKHKKICLLAVLSGFVPLSTIFISNSVNTQNNNVNKETNITKKITVNY", "aa_hexdigest": "a17d7094551b0bbed4fd4bf72272158d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5507.430900000002, "isoelectric_point": 10.01917667388916 }, "id": "IGIGPGHMMC_607", "locus": "GCA000148625_00580" }, { "type": "cds", "sequence": "contig_1", "start": 329011, "stop": 329178, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACTTCGATTTAGCAGCCGCTAAAAACAATAAAAATAACAAAAATGATAGCTTAGAATTAAAAATTAAGTATAGAAGTAAATCTAATTCATCAGAAGAAACTATTACATTTAAATCTAATAATTCCTCAAAATATACTGATATAAATTTATCATTTCCTGGTTGA", "aa": "MHFDLAAAKNNKNNKNDSLELKIKYRSKSNSSEETITFKSNNSSKYTDINLSFPG", "aa_hexdigest": "daa99df91ab7a66c49400ee36998f8f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6241.820900000001, "isoelectric_point": 9.398475074768069 }, "id": "IGIGPGHMMC_608", "locus": "GCA000148625_00581" }, { "type": "cds", "sequence": "contig_1", "start": 329383, "stop": 329667, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGGGAAGATATTAAGTTTGACCAAGATTACATAAAGAAAAAAATTAATACGCTTTTTGAATTATTATTTTGTAATACTCCTACACCTTACTCAGACAAAAGCTCTTTACACACCCCAGGAATTGATAGTGTATGGGAATATATAATAAAAAAAGGTTTTGAGAATAAAAAAGCATTTGAAAATTTTTTGCCTTTATATAACAATTTTTATAATAGTCTTTTTTCTAATGGGACAAAAGTAGACAAATATTTATCTGATAATTCGTTTAATATTCATTGA", "aa": "MKREDIKFDQDYIKKKINTLFELLFCNTPTPYSDKSSLHTPGIDSVWEYIIKKGFENKKAFENFLPLYNNFYNSLFSNGTKVDKYLSDNSFNIH", "aa_hexdigest": "0eb3687423cb34acd2f557e64c401fd9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11159.49289999999, "isoelectric_point": 7.797144889831544 }, "id": "IGIGPGHMMC_609", "locus": "GCA000148625_00582" }, { "type": "cds", "sequence": "contig_1", "start": 329782, "stop": 330144, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCTTTTCTTTCATCTTGTTTCAGACATGAATATAAACCTTTTTTTATAAGTATTTCTGGATCTTCGCAAGGTGTATATGTTAGTTTTGATTCTTACGATTATTCAGAATTCTTGCATGAATTATTGCATGAATTATCAAAATATATTCTTTACTATTATTATTATAGAATTGTTGATTTTGAATATTACGATAATAAAAAATTCTTTGAGGATAGTCTTGGTTTGCACCCAGAGCTATATAAAGAAATGGAGAAAGAAGGTGGCCCTCTTTACCATATATTTTTCTTAAAAGAATTTATAAATTTTGGAATTAAATCCTTTGAAATGGAAGACTACAAAAAAGTTAAAAAATTTTGA", "aa": "MYSFLSSCFRHEYKPFFISISGSSQGVYVSFDSYDYSEFLHELLHELSKYILYYYYYRIVDFEYYDNKKFFEDSLGLHPELYKEMEKEGGPLYHIFFLKEFINFGIKSFEMEDYKKVKKF", "aa_hexdigest": "8d5e4d557a14171c49479a1166bc0a7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14793.61979999999, "isoelectric_point": 5.5009572982788075 }, "id": "IGIGPGHMMC_610", "locus": "GCA000148625_00583" }, { "type": "cds", "sequence": "contig_1", "start": 330823, "stop": 331041, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATAGATATAAGTTTGCTACATTTAATATAAATATAAAACTAGATATTCTAAAACTAAAGAATGATTTTAAACCTGATAAAAATAAGAGTGCTAATGAAAAACTTCTCAATCATTTAGAAGCTTCTAATTTTGATGAACAAGATTTTTTAAATAAATATGTTAAAGCGACATCTAAAATAGATAAAAATGATCTAAAAATTAAAAATAAATGA", "aa": "MFYRYKFATFNINIKLDILKLKNDFKPDKNKSANEKLLNHLEASNFDEQDFLNKYVKATSKIDKNDLKIKNK", "aa_hexdigest": "4763359dd0d0d365f5b1220e5cfb01f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8614.838499999994, "isoelectric_point": 9.584917640686033 }, "id": "IGIGPGHMMC_611", "locus": "GCA000148625_00584" }, { "type": "cds", "sequence": "contig_1", "start": 331144, "stop": 331614, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAATTTAACGATGATGTTGAAACTATAAACAACAATAAGACAAAGACAATAAACGAATTTGAAGATATTATAAAACCATTAACTGTCGACAAATATGTTAAATCTCTTAAAAATTCAGAAACGGGTGAAGATATCAAAGATATTAATAAAATAATTAAGTATAGATATATTATAGATACCCATAAAAAACTAAAAATTAATATTAGTATGTTAAATAATGAATATTATATAAGTAATTTCTCAGAAAAAGAGTATGACGTTGATTTAGGAGCATTTGACGAAGCATATTTATCTGACTTTGAACCACCTGTTATTAATCTTTATGGCCGGAATACGATTGATTTAATTAAAAATTATCTTAAAAACACAAGCGAGTATGGTGTCTTACAAAGTTATTTAAATGAATATAATCGCAAAAAACTATCTGAAAACAACAAACTTTTTTCAAACAAAAAAACTAAATGA", "aa": "MLEFNDDVETINNNKTKTINEFEDIIKPLTVDKYVKSLKNSETGEDIKDINKIIKYRYIIDTHKKLKINISMLNNEYYISNFSEKEYDVDLGAFDEAYLSDFEPPVINLYGRNTIDLIKNYLKNTSEYGVLQSYLNEYNRKKLSENNKLFSNKKTK", "aa_hexdigest": "13ef4f0c78f0aac1c60c2769db209188", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18449.597900000004, "isoelectric_point": 5.579054069519042 }, "id": "IGIGPGHMMC_612", "locus": "GCA000148625_00585" }, { "type": "cds", "sequence": "contig_1", "start": 331858, "stop": 331977, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAAATTTTGAAAAAATTAAGAAAGAAGCAAAAGAACAAATTACATATAAAATTAAAGAAGCTTTTGGCCCAAATTCAAAGCTTGTACAAAATAATTTATATGATTTTTTATGA", "aa": "MDKNFEKIKKEAKEQITYKIKEAFGPNSKLVQNNLYDFL", "aa_hexdigest": "be59a369bf074fa0f8ff0263594f7270", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4664.337200000002, "isoelectric_point": 9.050087928771973 }, "id": "IGIGPGHMMC_613", "locus": "GCA000148625_00586" }, { "type": "cds", "sequence": "contig_1", "start": 332050, "stop": 332271, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGATAATGGCGTTGACTTTCAGATAGTTGTTAAAAATTTAAAAGCAACTAAACCATATGACTTAAATAAACTAGAAATTGATACTGTTACAACTAGTGAAGAAAACAAGGAAAAACTTATTGATTCTGTTTATAAAATAATAGAAAAAAATACTCTTATTCCAAAAGAAGAATTAAAAAACAAGACAGAATTAATTGTTGAAGATGGTAATTGA", "aa": "MQDDNGVDFQIVVKNLKATKPYDLNKLEIDTVTTSEENKEKLIDSVYKIIEKNTLIPKEELKNKTELIVEDGN", "aa_hexdigest": "f43229693a07c356eab0d171593ac7b6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8394.422499999995, "isoelectric_point": 4.644280052185059 }, "id": "IGIGPGHMMC_614", "locus": "GCA000148625_00587" }, { "type": "cds", "sequence": "contig_1", "start": 332611, "stop": 333357, "strand": "+", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAACAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAGTTCATTGCTGTTAACTTAATCAAAAATATGATTAATCAATTAATTGATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTGTAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAGGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCTAGACTTGCAAGCAAATACAATGTAAGCAAAACTCAAATATACAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAGAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACCTTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACAATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCCAGCAAATGTTTATCAAAAACAATTAAAGAAGCAATAACAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAVNLIKNMINQLIDYKYSDERKSKYKAHDVIVRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRLASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISSKCLSKTIKEAITKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "b350ac9d7d217e4ba9aaeb16a0727721", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 332536, "stop": 333660, "observations": { "mutation": [ 333355, 333499 ], "stop": [ 333355, 333499 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 0.976, "score": 477.0, "evalue": 3.02e-167, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9679144385026738, "score": 725.0, "evalue": 1.84e-267, "description": "Internal stop codon at 333355, 333499. Nonsense mutation around 333355, 333499.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "IGIGPGHMMC_615", "locus": "GCA000148625_00588" }, { "type": "cds", "sequence": "contig_1", "start": 333553, "stop": 333828, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGTGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY", "aa_hexdigest": "25f9e36794dd6b75a6d134d610038f68", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8053097345132744, "identity": 0.9890000000000001, "score": 174.0, "evalue": 4.75e-55, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10689.122099999988, "isoelectric_point": 7.929176139831544 }, "id": "IGIGPGHMMC_616", "locus": "GCA000148625_00589" }, { "type": "cds", "sequence": "contig_1", "start": 334030, "stop": 334692, "strand": "+", "frame": 1, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244E65B6", "UniRef:UniRef90_UPI002852BFD7" ], "nt": "ATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAG", "aa": "MNKNVEEIIYNAMEDYKIDIIIGPEGNSKVMRMNLKPFTLIGATTKINLLTQPLKDRFGFIARLSQYNEKEIAKILENSEMVLNIKSERGVIPLIAKYSKGTPRVANHLLKRILDFSIQNKEDEITKKTTYSTFKHLELYDLGLGREHIEYLKVLNETFENKFAAIDSIAGVLNIDRDILIYELEPLLLYLKLIAKGPRGRKITTKGIDYLLKNKLDIHY", "aa_hexdigest": "0ab81765b35b1b27df9a2143403d611c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002852BFD7", "query_cov": 1.0, "subject_cov": 0.9322033898305084, "identity": 1.0, "score": 432.0, "evalue": 4.52e-153, "valid": true, "product": "Holliday junction DNA helicase RuvB C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI00244E65B6" }, "pscc": { "uniref50_id": "UniRef50_UPI00244E65B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244E65B6" ], "product": "Holliday junction DNA helicase RuvB C-terminal domain-containing protein" }, "genes": [ "ruvB" ], "id": "IGIGPGHMMC_617", "locus": "GCA000148625_00590" }, { "type": "cds", "sequence": "contig_1", "start": 334895, "stop": 335356, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGA", "aa": "MSIEYGGGVEILVQAQKNGKKVNDKYAKNIANTMDDRLTGGIAFNGTTVNVEGDGILKITQNGKLSSKEKEELEKIVSSKPNLILTDGDMIPLFVRGKFNESREKIDYSRIEDYIPPLKENGAKFKFIPPSEKGRSGYYGIDVSLRNNAAADE", "aa_hexdigest": "4667b0b884826d93bf19f3a9421556b0", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16875.891599999984, "isoelectric_point": 6.404697227478027 }, "id": "IGIGPGHMMC_618", "locus": "GCA000148625_00591" }, { "type": "cds", "sequence": "contig_1", "start": 335618, "stop": 336259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02355.23" ], "nt": "TTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGA", "aa": "MKGTQFFISGRFNEAQAKKTAANINFGSANYDLKILSSNYIEASKTSNSFRSALLAGAVVFSIIAVFMIVNYGLLGALSTISIALYMFLTLLLFTVLRGEYSPVTIAALIIGIGISVDSNIITFERLKNEVYAGDRLKKAMKNANHRTLGSILDANILTLLISFVLFFFGTSTIKGFSISLVFSVTFILIVMLIFTRGLSVLLVNTGAFDKKL", "aa_hexdigest": "2a71420e1fa4ac53e07a7acd7d525e25", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02355.23", "name": "SecD_SecF_C", "length": 161, "aa_cov": 0.7417840375586855, "hmm_cov": 1.0, "evalue": 4.1635247098388525e-15, "score": 51.66889190673828, "start": 30, "stop": 187 } ], "seq_stats": { "molecular_weight": 23212.17610000001, "isoelectric_point": 9.897847175598145 }, "id": "IGIGPGHMMC_619", "locus": "GCA000148625_00592" }, { "type": "cds", "sequence": "contig_1", "start": 336446, "stop": 336556, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGA", "aa": "MEFGGGVRISIESANSAYVNSDILDIQNKFMAHMNK", "aa_hexdigest": "bc270f5c0f2dd1d01024fd82a607eb59", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3988.4829, "isoelectric_point": 5.4648645401000975 }, "id": "IGIGPGHMMC_620", "locus": "GCA000148625_00593" }, { "type": "cds", "sequence": "contig_1", "start": 336596, "stop": 336832, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGA", "aa": "MDLGNGNFKLQIETSQDLSNVLSKIESDVATFVSSGNKPRFIAYAISTSRAFDLLKYSMIAIAGAFLVVIVYTFIRFK", "aa_hexdigest": "0ca290aeffc772b4f154dd0e124788c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8643.935499999996, "isoelectric_point": 9.156267356872558 }, "id": "IGIGPGHMMC_621", "locus": "GCA000148625_00594" }, { "type": "cds", "sequence": "contig_1", "start": 336878, "stop": 337231, "strand": "+", "frame": 2, "gene": null, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "nt": "ATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGA", "aa": "MSIAFIVVARLQLSPIMIAALLSITAFSINDTIIVFERIKETINTQYHGVFLSKALIKEIANNAIKDTFKRSLYTTLTTVVSILVLLMFGNATDLSFNIVMIFGLVVGSYSSIFICT", "aa_hexdigest": "b62689f9cb1c4fc95ff0771e77b94d8f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9G7", "query_cov": 0.9743589743589743, "subject_cov": 0.9193548387096774, "identity": 0.579, "score": 109.0, "evalue": 9.97e-29, "valid": false, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A432F9G7" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9G7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_622", "locus": "GCA000148625_00595" }, { "type": "cds", "sequence": "contig_1", "start": 337459, "stop": 337764, "strand": "+", "frame": 1, "gene": "truB", "product": "TruB family pseudouridylate synthase (N terminal domain)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1", "UniRef:UniRef90_UPI000FF45FC1" ], "nt": "ATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGA", "aa": "MFYLIKKTKGISSFSCIKTFAKKMNIKKIGHSGTLDPLASGLLLCASEDDTKLIPYISHKSKTYISKIIFGKHTSTYDSEGEITNTSEVKIKEEDLEKINK", "aa_hexdigest": "22f91c7a57129b1d5b2754c594a64c1a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF45FC1", "query_cov": 1.0, "subject_cov": 0.8859649122807017, "identity": 1.0, "score": 197.0, "evalue": 5.24e-64, "valid": true, "product": "TruB family pseudouridylate synthase (N terminal domain)", "uniref50_id": "UniRef50_UPI000FF45FC1" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF45FC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1" ] }, "genes": [ "truB" ], "id": "IGIGPGHMMC_623", "locus": "GCA000148625_00596" }, { "type": "cds", "sequence": "contig_1", "start": 338017, "stop": 338310, "strand": "+", "frame": 1, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "db_xrefs": [ "EC:5.4.99.25", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "KEGG:K03177", "RefSeq:WP_259637334.1", "SO:0001217", "UniParc:UPI000FEDE12C", "UniRef:UniRef100_UPI0001E330A7", "UniRef:UniRef50_A0A3P8LB52", "UniRef:UniRef90_C4XEQ3" ], "nt": "ATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTGATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAA", "aa": "MSELERIKIGKLALSDTKNEYEQIEINNLFDLDFYNATDSEIESLKKGQVINANKKLKDNKYLLINKTKDQNLILGILELKNNNLKVVKLFGNRLEE", "aa_hexdigest": "2f748c8b3af80dd7e63dc3300c270c2a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000FEDE12C", "ncbi_nrp_id": "WP_259637334.1", "uniref100_id": "UniRef100_UPI0001E330A7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FEDE12C", "RefSeq:WP_259637334.1", "UniRef:UniRef100_UPI0001E330A7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330A7", "uniref90_id": "UniRef90_C4XEQ3" }, "psc": { "uniref90_id": "UniRef90_C4XEQ3", "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "ec_ids": [ "5.4.99.25" ], "uniref50_id": "UniRef50_A0A3P8LB52", "kegg_orthology_id": "K03177", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LB52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB52" ], "product": "tRNA pseudouridine synthase B" }, "genes": [ "truB" ], "id": "IGIGPGHMMC_624", "locus": "GCA000148625_00597" }, { "type": "cds", "sequence": "contig_1", "start": 338313, "stop": 338681, "strand": "+", "frame": 3, "gene": null, "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7", "UniRef:UniRef90_A0A3P8LIG5" ], "nt": "ATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGA", "aa": "MKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYG", "aa_hexdigest": "f68b02b222dbfe34258929425b1c9a06", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 338283, "stop": 338984, "observations": { "mutation": [ 338679, 338709, 338886, 338943 ], "stop": [ 338679, 338709, 338886, 338943 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LIG5", "query_cov": 1.0, "subject_cov": 0.4206896551724138, "identity": 1.0, "score": 245.0, "evalue": 5.03e-80, "gene_start": 1, "gene_end": 122, "reference_start": 11, "reference_end": 132, "reference_sequence": "MIWQPIRGVIMKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYGWYSPKMNINTWFLSPHQAKMKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQWATGVFVSPKGITKSHTLDWLAKYLKLDSKKNLIAFGDSSNDYEMIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK" }, "paralog": false, "identity": 0.9785407725321889, "score": 455.0, "evalue": 5.83e-165, "description": "Internal stop codon at 338679, 338709, 338886, 338943. Nonsense mutation around 338679, 338709, 338886, 338943.", "psc": { "uniref90_id": "UniRef90_A0A3P8LIG5", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LIG5", "UniRef:UniRef50_A0A449B5A7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7" ], "product": "COF family HAD hydrolase protein" } }, "id": "IGIGPGHMMC_625", "locus": "GCA000148625_00598" }, { "type": "cds", "sequence": "contig_1", "start": 338736, "stop": 338888, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGA", "aa": "MKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQ", "aa_hexdigest": "19fc09a76ae14d87ebb826c9a48e764f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5734.4913, "isoelectric_point": 5.507266426086424 }, "id": "IGIGPGHMMC_626", "locus": "GCA000148625_00599" }, { "type": "cds", "sequence": "contig_1", "start": 339018, "stop": 339155, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAA", "aa": "MIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK", "aa_hexdigest": "feb24920720995d9d47f54d63584c650", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4965.651199999998, "isoelectric_point": 4.716181373596191 }, "id": "IGIGPGHMMC_627", "locus": "GCA000148625_00600" }, { "type": "cds", "sequence": "contig_1", "start": 339175, "stop": 340047, "strand": "+", "frame": 1, "gene": null, "product": "FAD synthase", "db_xrefs": [ "RefSeq:WP_013354548.1", "SO:0001217", "UniParc:UPI0001E330A9", "UniRef:UniRef100_C4XEQ5", "UniRef:UniRef50_C4XEQ5", "UniRef:UniRef90_C4XEQ5" ], "nt": "ATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAG", "aa": "MSLKVYTFDNIPKFQKPIFIIGSFESFHLGHYKLLEKARELAKESEKERDIVLVYFEDIENLSKTKNLIFSDAKNRVQEFANLKINFAISLFYSQISHLSAQDFIDKLIANQDDYSFVIGEEFKFGFKRSGNVQFLENHYNKDKVFAQEVVRLKNKQKISTSYLKELIEFGDIDLLNTINMFPYSFSAKFSTIENKIELKKHELLIPLRQGIYAVLVELNNYNYYALMQVNFDTKYFIEFLDFKIKENQNIDARIKVLKAIRFFRNKELESINEQNKLNAKSILLDLNNN", "aa_hexdigest": "8be05d7590d4d50ccd4ba131fd99ec20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330A9", "ncbi_nrp_id": "WP_013354548.1", "uniref100_id": "UniRef100_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330A9", "RefSeq:WP_013354548.1", "UniRef:UniRef100_C4XEQ5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEQ5", "uniref90_id": "UniRef90_C4XEQ5" }, "psc": { "uniref90_id": "UniRef90_C4XEQ5", "product": "FAD synthase", "uniref50_id": "UniRef50_C4XEQ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ5" ], "product": "FAD synthase" }, "genes": [], "id": "IGIGPGHMMC_628", "locus": "GCA000148625_00601" }, { "type": "cds", "sequence": "contig_1", "start": 340262, "stop": 340852, "strand": "+", "frame": 2, "gene": null, "product": "nucleotidyltransferase", "db_xrefs": [ "EC:6.3.1.22", "KEGG:K27343", "SO:0001217", "UniRef:UniRef50_C4XEQ6", "UniRef:UniRef90_A0AB32XBA6" ], "nt": "ATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGCGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGA", "aa": "MSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDISAYSPMTFKKVPRTTASLY", "aa_hexdigest": "3c5e23f2342184eefd31e3cc39f8c98e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 340157, "stop": 341155, "observations": { "mutation": [ 340220, 340850, 341030, 341048 ], "stop": [ 340220, 340850, 341030, 341048 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBA6", "query_cov": 1.0, "subject_cov": 0.5798816568047337, "identity": 1.0, "score": 383.0, "evalue": 1.97e-132, "gene_start": 1, "gene_end": 196, "reference_start": 36, "reference_end": 231, "reference_sequence": "MAVGIIAEYNPFHNGHIKQINWVKEHFPGEKIVVVMSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDISAYSPMTFKKVPRTTASLYWKFQKIMNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVEACTSKRYTASRIQRIIAWILNRKWKKTSNRKVKKLLKFIKKKKHKILNQRVDKINFSFLLHLFF" }, "paralog": false, "identity": 0.9879518072289156, "score": 640.0, "evalue": 3.49e-236, "description": "Internal stop codon at 340220, 340850, 341030, 341048. Nonsense mutation around 340220, 340850, 341030, 341048.", "psc": { "uniref90_id": "UniRef90_A0AB32XBA6", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_C4XEQ6", "kegg_orthology_id": "K27343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBA6", "EC:6.3.1.22", "UniRef:UniRef50_C4XEQ6", "KEGG:K27343" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ6" ], "product": "tRNA(Met) cytidine acetate ligase" } }, "id": "IGIGPGHMMC_629", "locus": "GCA000148625_00602" }, { "type": "cds", "sequence": "contig_1", "start": 340868, "stop": 341032, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTTGAAGCATGTACTTCAAAAAGATATACAGCTTCAAGAATCCAAAGAATAATAGCTTGA", "aa": "MNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVEACTSKRYTASRIQRIIA", "aa_hexdigest": "dac9956a6a16b0839c58984e796d96f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6269.3001, "isoelectric_point": 9.917961311340331 }, "id": "IGIGPGHMMC_630", "locus": "GCA000148625_00603" }, { "type": "cds", "sequence": "contig_1", "start": 341222, "stop": 341629, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGA", "aa": "MDSTNKKKILEVQGLKKYFFNNNKINKAVDNVSFNLYEGQILGLIGESGSGKTTVGRSIAKLYDDFQGLLLVDGKLINNKKMSESDRKFLRKNIQMIFQNPKNSLDEQKSIYSILKESLIANNILKIKTKEIFKN", "aa_hexdigest": "5e0d2e4b6d055a4df5b2ba9abe5ff329", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9185185185185185, "subject_cov": 0.96875, "identity": 0.621, "score": 147.0, "evalue": 4.09e-43, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_631", "locus": "GCA000148625_00604" }, { "type": "cds", "sequence": "contig_1", "start": 341879, "stop": 342076, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGA", "aa": "MLEFYEQKQIDYTNGNFPFGIFDFIELENERKKALILTKMSKENYESMQLIDNLNNEMKNIKKEF", "aa_hexdigest": "d14b506dad54c79ae4b3d9f26d882cc5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.971699999996, "isoelectric_point": 4.8526517868041985 }, "id": "IGIGPGHMMC_632", "locus": "GCA000148625_00605" }, { "type": "cds", "sequence": "contig_1", "start": 342158, "stop": 342769, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGA", "aa": "MNNTLDEELYKFDYKKAFLAKMQIDLLNKNKKLINNLLVKDIKELIYDLKNYNLDFFNKYFQPLEFNKKIKENMQSTYETYYEFDFNKYIIRSNLNLTQYEEKIDHIKKLISKQMILSKNAGDVKNSIEYLNELKTKIEKIDLEFEDKLVNHKEETLKKVKQVETELDEKEKINNVLKIRQSNFYQNYSDIKLLKLFNTFLAN", "aa_hexdigest": "67a7453ca611bcae005df33fb02c1ac2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24647.12770000004, "isoelectric_point": 8.653800773620606 }, "id": "IGIGPGHMMC_633", "locus": "GCA000148625_00606" }, { "type": "cds", "sequence": "contig_1", "start": 342920, "stop": 343501, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAA", "aa": "MGLSHKNSKSFFGVFKDFFTNQKIKRNVKELLCKNIIYKALEDVGLLKQFAYRYPHEFSGGQLQRVAIARALIVEPKIIIADEPIASLDISIQAQVVNLLKELCEKRNIGVIFIAHDLSMIEYLADEVQIMHHGNLLKAVKPILSTKNQCTLILKTYLPQFLELKMLIKNLSTKTLKLNISKNKNFLIQLIYT", "aa_hexdigest": "891f1361b957c9b40e11825df7e6051b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 69, "aa_cov": 0.34196891191709844, "hmm_cov": 0.9420289855072463, "evalue": 6.543206782018892e-13, "score": 45.487030029296875, "start": 21, "stop": 86 } ], "seq_stats": { "molecular_weight": 22116.09070000003, "isoelectric_point": 9.572668647766111 }, "id": "IGIGPGHMMC_634", "locus": "GCA000148625_00607" }, { "type": "cds", "sequence": "contig_1", "start": 344001, "stop": 344198, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTAGATACAATTAGTTCACTTGCTTTATTAACTCCAGTTTTGGCTGCTAGTTGTAAGCCAATTTTAGTTGTTAAAGAAGAATATAACAAGGAAAAAAGTTATCTTAAAACATTTCTAAAAACTATTAATAGCAAAAGCGATCTAGAAAAAAATGAATTCAAAAAATTTAGAATTAATTTTCATACTTTTTAA", "aa": "MILDTISSLALLTPVLAASCKPILVVKEEYNKEKSYLKTFLKTINSKSDLEKNEFKKFRINFHTF", "aa_hexdigest": "106acd6d13e317bcbc94095e529e3111", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7538.819599999997, "isoelectric_point": 9.374750709533693 }, "id": "IGIGPGHMMC_635", "locus": "GCA000148625_00608" }, { "type": "cds", "sequence": "contig_1", "start": 344400, "stop": 344696, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7", "UniRef:UniRef90_UPI000FEECEC7" ], "nt": "TTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGA", "aa": "MPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQE", "aa_hexdigest": "c9c5ba0b2045cbcfddbd7c7a8d9c4e8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 344313, "stop": 344828, "observations": { "mutation": [ 344322, 344694 ], "stop": [ 344322, 344694 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECEC7", "query_cov": 1.0, "subject_cov": 0.5664739884393064, "identity": 0.99, "score": 176.0, "evalue": 7.38e-55, "gene_start": 1, "gene_end": 98, "reference_start": 30, "reference_end": 127, "reference_sequence": "MPIWGQILLVLFSSIFLSTIFVVLTYKFFLPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQEWIESNKGYEEEFTFYNKAYEYQIDFFDFDLKSAKYMVVESNNLLRL" }, "paralog": false, "identity": 0.9824561403508771, "score": 325.0, "evalue": 4.87e-116, "description": "Internal stop codon at 344322, 344694. Nonsense mutation around 344322, 344694.", "psc": { "uniref90_id": "UniRef90_UPI000FEECEC7", "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECEC7", "UniRef:UniRef50_UPI000FEECEC7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11468.611399999989, "isoelectric_point": 9.673819541931152 }, "id": "IGIGPGHMMC_636", "locus": "GCA000148625_00609" }, { "type": "cds", "sequence": "contig_1", "start": 344799, "stop": 345053, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAG", "aa": "MVVESNNLLPALKVKHFISFKKLQKIYKKTLHIQLLENECYLYSLINTDHCFIKKTKVKNIQYFALLQDFIEKFKKVRAFLNLN", "aa_hexdigest": "4efa76a6fa17cc2ee86ba8a17d6f4548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10091.987599999991, "isoelectric_point": 9.732163429260254 }, "id": "IGIGPGHMMC_637", "locus": "GCA000148625_00610" }, { "type": "cds", "sequence": "contig_1", "start": 345144, "stop": 345479, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0", "UniRef:UniRef90_C4XER0" ], "nt": "ATGCAAAGAAAAAAAATCAAAAATATTTTAATTGTTTCTTCTTTTGCTATTCCAATTTCAATGACTATTTCAGCAAGTGGCTGCAATTTAAAAGCTATTGAAAATAAATTCAATAATGAGATAGTGAAATCAAAAGAATGTGTCAAAGAATTTGAAAATAAAAAAGATATTCAAGAAGAAAAATATGAAGAATTAAGAGAAAGTTTAGTGAATTTTTCTAAATATATTGTTCAAGTAGATACTTTAAAACCTGGTCCAGTATCACTAAGAAATAGAAAAATATATCAATATGCAACAAGATTACTTCAAAGTTTTCAACAATTGCATAAAAATTGA", "aa": "MQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKN", "aa_hexdigest": "716207286e8245e04cf5dfd285df89da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 345057, "stop": 345485, "observations": { "mutation": [ 345080, 345146 ], "stop": [ 345080, 345146 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER0", "query_cov": 1.0, "subject_cov": 0.7816901408450704, "identity": 1.0, "score": 209.0, "evalue": 5.24e-68, "gene_start": 1, "gene_end": 111, "reference_start": 3, "reference_end": 113, "reference_sequence": "MEMQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKNWYEYILKNGNDENCDDKMTQELWDFYLKK" }, "paralog": false, "identity": 0.9788732394366197, "score": 276.0, "evalue": 7.62e-97, "description": "Internal stop codon at 345080, 345146. Nonsense mutation around 345080, 345146.", "psc": { "uniref90_id": "UniRef90_C4XER0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER0", "UniRef:UniRef50_C4XER0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0" ], "product": "Lipoprotein" } }, "id": "IGIGPGHMMC_638", "locus": "GCA000148625_00611" }, { "type": "cds", "sequence": "contig_1", "start": 345788, "stop": 346351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATGTAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGA", "aa": "MKKANKLIISLGTAFAALSPVVLSVGCGGGGSSNLDSINGESTPSNENSAYANDKKGYNIKANDKVQIATTFSKGRAQYEALNDVIKNYNRLMKDQPGFKEVEQVSLGSGYDGQADVVRKKITAKEAQMPNLLLNYSSLASSLAESGVLLNFTTKENGGKESISNSDFAESFSRANRTTSKINNGGS", "aa_hexdigest": "2c60a0b16416f10eb7c3d1d9891787b1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19815.840800000005, "isoelectric_point": 9.07793827056885 }, "id": "IGIGPGHMMC_639", "locus": "GCA000148625_00612" }, { "type": "cds", "sequence": "contig_1", "start": 346637, "stop": 346759, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGA", "aa": "MFEKSSAVTTLEGPHALGIDDITGFIQTLGYASVGADPES", "aa_hexdigest": "7925ceb9795947445814e282e18d460b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4126.508000000001, "isoelectric_point": 4.0500284194946286 }, "id": "IGIGPGHMMC_640", "locus": "GCA000148625_00613" }, { "type": "cds", "sequence": "contig_1", "start": 346814, "stop": 347053, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGGTATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGA", "aa": "MEATSQANSKFKELYDMFVPKLKSQSVVFQQSGAYTSSEQIRHNFMFGIGSTAGYTHNFAKKSETSIEIKNSEGKFSSG", "aa_hexdigest": "1a9513a8c98dbbc81e859a3546bded5e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8782.685499999998, "isoelectric_point": 9.005153465270997 }, "id": "IGIGPGHMMC_641", "locus": "GCA000148625_00614" }, { "type": "cds", "sequence": "contig_1", "start": 347276, "stop": 347611, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGA", "aa": "MIETKNKDDIKTLDAALADGKLVKVGEFVNNQNKEAILYLAFGKGAGISGLKIVEKGLDSLLEKDEFFKFATPSKTKDTDAKNVYYAQGPSLFGIRSSDNAMNQATRMFVK", "aa_hexdigest": "df6c7e4fc1bd61b7dac4879eb2adff91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12183.815599999984, "isoelectric_point": 8.776741981506348 }, "id": "IGIGPGHMMC_642", "locus": "GCA000148625_00615" }, { "type": "cds", "sequence": "contig_1", "start": 347696, "stop": 347893, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGA", "aa": "MFSEQASYIFAVKDFEKTPMTTTNPYLRECFEMFKKTIEDPEHNAIYEEYGDPNGDAFRGALGTA", "aa_hexdigest": "ccd26344ed9bce850cc6cb66f87f58e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.2107, "isoelectric_point": 4.482516288757325 }, "id": "IGIGPGHMMC_643", "locus": "GCA000148625_00616" }, { "type": "cds", "sequence": "contig_1", "start": 348072, "stop": 348482, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGA", "aa": "MKFKNNTLNFCLNTIVPSMAIVVCSSCQSKDDKIYNEAFKEDIKFDYVAYNPATGKYDIVDKNIENQLTTSLNNLSNISREFTYDGEEYKLEIDTISLARTFDKNKTFSENLKDPNFINNQREYTYKIFKKNPKGK", "aa_hexdigest": "5ab25517ebd08e93a55a015ffe71c73a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15859.692099999982, "isoelectric_point": 7.595303535461427 }, "id": "IGIGPGHMMC_644", "locus": "GCA000148625_00617" }, { "type": "cds", "sequence": "contig_1", "start": 348513, "stop": 348614, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGA", "aa": "MTKKFDVKLNLVDNFDKMDTRIDYSKLQATAYD", "aa_hexdigest": "f428e6a48ff83c30e7cda2c2c0749920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3928.4445000000005, "isoelectric_point": 6.022057151794433 }, "id": "IGIGPGHMMC_645", "locus": "GCA000148625_00618" }, { "type": "cds", "sequence": "contig_1", "start": 348720, "stop": 348920, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "ATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGA", "aa": "MVVETIRIQGVDTPEKAVGKEIAPIFEKRFAEKSSEFANVNLPAGTKVRFIYSNKDAFKRIVAHLF", "aa_hexdigest": "33a7fc38db56d2043591462342e6f0ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 61, "aa_cov": 0.9242424242424242, "hmm_cov": 0.9180327868852459, "evalue": 1.6740919420766169e-06, "score": 24.523019790649414, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7453.5775, "isoelectric_point": 9.519997978210448 }, "id": "IGIGPGHMMC_646", "locus": "GCA000148625_00619" }, { "type": "cds", "sequence": "contig_1", "start": 348945, "stop": 349250, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAG", "aa": "MYEYSAAITRFGFTLPYADGTELINMTNAGKTQHYTFLQIGEAFEAAMAEKTGFFKCLSDPDDISRYVYLSKRNGKYTIFQKKSNSNVFKFKDKAYKNPIN", "aa_hexdigest": "5661ce40177eeabb1f7757512e22f194", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11612.045599999985, "isoelectric_point": 9.162327384948732 }, "id": "IGIGPGHMMC_647", "locus": "GCA000148625_00620" }, { "type": "cds", "sequence": "contig_1", "start": 349294, "stop": 349734, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGA", "aa": "MIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDAD", "aa_hexdigest": "90bd6f8ed3309e634846891e505fe558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.547945205479452, "hmm_cov": 0.9634146341463414, "evalue": 8.794777379667007e-21, "score": 70.98065948486328, "start": 61, "stop": 140 } ], "seq_stats": { "molecular_weight": 16553.856099999983, "isoelectric_point": 9.329300498962404 }, "id": "IGIGPGHMMC_648", "locus": "GCA000148625_00621" }, { "type": "cds", "sequence": "contig_1", "start": 349945, "stop": 350835, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3", "UniRef:UniRef90_C4XER3" ], "nt": "ATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAATAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGA", "aa": "MSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQ", "aa_hexdigest": "98f83c74bab74ce76359384bfbb2f653", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 349645, "stop": 351138, "observations": { "mutation": [ 349732, 349843, 350833 ], "stop": [ 349732, 349843, 350833 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER3", "query_cov": 1.0, "subject_cov": 0.42105263157894735, "identity": 1.0, "score": 522.0, "evalue": 4.43e-180, "gene_start": 1, "gene_end": 296, "reference_start": 223, "reference_end": 518, "reference_sequence": "MKGKNMIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDADWQNKVLDRKTEAIINIRNIYLKSLGASDLEIKQINEAWKIYKSIYRETQSELANYRVELREKFDKAFTDYKMSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQWIRDIQQKLGITTVFVTHDQEEAMSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK" }, "paralog": false, "identity": 0.993963782696177, "score": 940.0, "evalue": 0.0, "description": "Internal stop codon at 349732, 349843, 350833. Nonsense mutation around 349732, 349843, 350833.", "psc": { "uniref90_id": "UniRef90_C4XER3", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER3", "UniRef:UniRef50_C4XER3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_649", "locus": "GCA000148625_00622" }, { "type": "cds", "sequence": "contig_1", "start": 350902, "stop": 351390, "strand": "+", "frame": 1, "gene": null, "product": "TOBE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "nt": "ATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAA", "aa": "MSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK", "aa_hexdigest": "803def7f3858fe188acb441811a784e7", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C8186B", "query_cov": 0.808641975308642, "subject_cov": 0.9492753623188406, "identity": 0.534, "score": 130.0, "evalue": 7.14e-36, "valid": false, "product": "TOBE domain-containing protein", "uniref50_id": "UniRef50_UPI0024C8186B" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C8186B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "product": "TOBE domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_650", "locus": "GCA000148625_00623" }, { "type": "cds", "sequence": "contig_1", "start": 351518, "stop": 351772, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTGTTAGTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGA", "aa": "MILLVPTLLVITAFTLVPFIINIIQSFTVQNAKNGVLHNFESLFKEPKFAVGVRNSILYAIVVLPFVMMVSLLISSCIANVYRK", "aa_hexdigest": "fc78a788d41c33ffd4f43b72ce2b7b31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9383.324999999999, "isoelectric_point": 9.744928169250485 }, "id": "IGIGPGHMMC_651", "locus": "GCA000148625_00624" }, { "type": "cds", "sequence": "contig_1", "start": 352019, "stop": 352399, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGA", "aa": "MLSVDKNLYKSASIDGIGPVKQFFTITLPSIKSTTTFLVTMGIINGIKVFPLALFNNRPEEAVLKGASTLMIFVYYYTSLGGAENLSLAAAGSILLFIIGIVYSTIIRGGFGTIMRVSLNLGESNV", "aa_hexdigest": "8b9d57ddc326d6fd3e561ff740d2ff53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13488.749299999985, "isoelectric_point": 9.346255683898928 }, "id": "IGIGPGHMMC_652", "locus": "GCA000148625_00625" }, { "type": "cds", "sequence": "contig_1", "start": 352497, "stop": 352655, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGA", "aa": "MLLGLFLKLLALTFFALIILFPFFFMLMISLMPETQAKTLQSSFSIIPKSLT", "aa_hexdigest": "c9463c2ae26de4d5c9eac5ef58a0baf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5928.348499999999, "isoelectric_point": 9.700251579284668 }, "id": "IGIGPGHMMC_653", "locus": "GCA000148625_00626" }, { "type": "cds", "sequence": "contig_1", "start": 352914, "stop": 353147, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGA", "aa": "MALKNNFIGAMVIIAVPFVASIFNALMFKNAFESIPSRIKEVALVDGAAGAKYLFKIAIPMVTPTTLTVVILTALAA", "aa_hexdigest": "b984adcf4cc5919a3b14d1bcd3edaec6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8140.814599999996, "isoelectric_point": 9.69799518585205 }, "id": "IGIGPGHMMC_654", "locus": "GCA000148625_00627" }, { "type": "cds", "sequence": "contig_1", "start": 353268, "stop": 353375, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAG", "aa": "MAGAVLVIIPMFIFYFAFRKRIMNAISRQGSTIKG", "aa_hexdigest": "cc64338f5b148f1a69f97488c4a299f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3948.8095999999996, "isoelectric_point": 11.733906745910645 }, "id": "IGIGPGHMMC_655", "locus": "GCA000148625_00628" }, { "type": "cds", "sequence": "contig_1", "start": 353477, "stop": 353644, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER6", "UniRef:UniRef90_C4XER6" ], "nt": "TTGCATAATACTATTCAAGTTTTAAAATTATCTGAATTTATTAGTTTCGACAAAGACTATCTACATGAAGCTATGTCACAATATTCATTTGCAATTGCAATAATTACATTTAGCTCAATCATAATTCTAATAGGTACTTATATTTCATATGCAGGATTCAAAAGTTGA", "aa": "MHNTIQVLKLSEFISFDKDYLHEAMSQYSFAIAIITFSSIIILIGTYISYAGFKS", "aa_hexdigest": "f1370a9df78f2b0e7c67b8dbc1fcd9cd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 353372, "stop": 353665, "observations": { "mutation": [ 353423, 353642 ], "stop": [ 353423, 353642 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER6", "query_cov": 1.0, "subject_cov": 0.5670103092783505, "identity": 0.982, "score": 103.0, "evalue": 1.04e-27, "gene_start": 1, "gene_end": 55, "reference_start": 36, "reference_end": 90, "reference_sequence": "MGSEIMKKANIKTKTIIWLILAIIAFILLIIFSVVLHNTIQVLKLSEFISFDKDYLHEAMSQYSFAIAIITFSSIIILIGTYISYAGFKSWKYNATI" }, "paralog": false, "identity": 0.9690721649484536, "score": 175.0, "evalue": 1.39e-58, "description": "Internal stop codon at 353423, 353642. Nonsense mutation around 353423, 353642.", "psc": { "uniref90_id": "UniRef90_C4XER6", "uniref50_id": "UniRef50_C4XER6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER6", "UniRef:UniRef50_C4XER6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6268.210099999997, "isoelectric_point": 5.977665901184082 }, "id": "IGIGPGHMMC_656", "locus": "GCA000148625_00629" }, { "type": "cds", "sequence": "contig_1", "start": 353674, "stop": 353850, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGA", "aa": "MNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPY", "aa_hexdigest": "c912bcb3fc7340d47c9f219846e6fca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6705.986799999999, "isoelectric_point": 9.76465549468994 }, "id": "IGIGPGHMMC_657", "locus": "GCA000148625_00630" }, { "type": "cds", "sequence": "contig_1", "start": 353893, "stop": 354921, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7", "UniRef:UniRef90_C4XER7" ], "nt": "TTGGCTTACAACTTTTTTGCTCCACATCGTGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGA", "aa": "MAYNFFAPHRAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNK", "aa_hexdigest": "50a6cd77fe95201787ece5d183be7d81", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 353662, "stop": 355170, "observations": { "mutation": [ 353848, 354919, 355042 ], "stop": [ 353848, 354919, 355042 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER7", "query_cov": 1.0, "subject_cov": 0.6812749003984063, "identity": 0.9940000000000001, "score": 674.0, "evalue": 4.38e-242, "gene_start": 1, "gene_end": 342, "reference_start": 78, "reference_end": 419, "reference_sequence": "MRRIMNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPYWLQKNAKDKTSNQLELAYNFFAPHHAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNKWKIFSLSNFFDNLRKKFDRTNPVDEEFTKDKLITYDAFPSDWTFDLPNTVTKDDVDKMSIYLENGVKELKFTLTKIKQYSNIK" }, "paralog": false, "identity": 0.9880478087649402, "score": 998.0, "evalue": 0.0, "description": "Internal stop codon at 353848, 354919, 355042. Nonsense mutation around 353848, 354919, 355042.", "psc": { "uniref90_id": "UniRef90_C4XER7", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER7", "UniRef:UniRef50_C4XER7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7" ], "product": "Lipoprotein" } }, "id": "IGIGPGHMMC_658", "locus": "GCA000148625_00631" }, { "type": "cds", "sequence": "contig_1", "start": 355251, "stop": 355943, "strand": "-", "frame": 3, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGAACCCAAATAGCGAAAGAGTTGACTTTGTTATTAAAATGCCTAATGGAGAAGCTAACTCTCAAAATGATCGTTACTTGCCTATTGATTGTAAGTTTCCACTTGATTCTTTTAATGCATATCAAGATGCAGACTCTAAAGAAGAAAGAATTAAAGCTCAAATTAACTTTAGAAAAGTTATTCTCGAACAAGCTAAAAGTATTAAAAGTAAATACATTATTAAAAATGTAACTACCAAGCAAGCAATTATGTATCTACCATCTGAAGCTATTTTTGCTTTAGCAGTTAGTGACAATGAATTGGTTTCAGCATTGAATAAAGATCATGATATTATGTTGGCTGGACCAAGTACAATTTTGATGATTATTCAAACCATTGCTTATGCAAATTATGGAATTAACATAAAACAAAATATTGACATTCTTTTAGATGCATTCTTATTTATTCGTCAAAGTGAAGATAATATTTATACCAAAATTGAACAAAGTATCAAGAAAAATGATAAAACAGGAGTTGAATTAAATAGAGCATTAAAAATAGTCGATAGAGTAAAGAAAAGAATTCAAAATTCTATTGATAGTGCACGAAAAAAAGGTATTATAAAAGAAGATTATGACATTGATAAAGTTGCAATGAATATGCCTATGATTGAAGATTTAAGTGATGAAAATGATGATAAAGAAGAAGAATAA", "aa": "MNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE", "aa_hexdigest": "fd352c1047aa627bcd1878a783a3777e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rmuC" ], "truncated": "3-prime", "pseudogene": { "start": 355251, "stop": 356243, "observations": { "mutation": [ 356018 ], "stop": [ 356018 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.5287356321839081, "identity": 1.0, "score": 431.0, "evalue": 2.12e-149, "gene_start": 1, "gene_end": 230, "reference_start": 206, "reference_end": 435, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.996969696969697, "score": 612.0, "evalue": 6.11e-223, "description": "Internal stop codon at 356018. Nonsense mutation around 356018.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "IGIGPGHMMC_659", "locus": "GCA000148625_00632" }, { "type": "cds", "sequence": "contig_1", "start": 356016, "stop": 356558, "strand": "-", "frame": 3, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGGAAATAGGAATTCTAATTGTAGTAATATTGACTATGATTTTAGCTATTAGTAGTATTGTCGTTAGTTTCATCTTATATAAAAAAAATAAGACAGAAGTTATCGATCAAGACAAAATGACAAATAATATTAAAAATCAAATAACTGAAGATTTGTCTAGTCAATTTGTCAAATTCAATAAAAATGTTGATGAAAAAATTGCCAATTCATTCAACATGATTAATAAAAACTTTAGTGATTTAAGAGAAGAAATTGACAAAAAAATGAAATCGTTTGATGATTTAGTAATTAAGGAAGTGCAAATAATTAAAGGTGCTATTGATGAAAAATTTGAAAATGAAGTAGCTAAAAAGATGGAAGAAAACTTTAAAAATATTAAAGAAAACATGGAAAATCTTGACAAGAATTTAGTACGTTTTGAGACCTTACAAGATTCAGTTAATTCATTACAAAAAACTTTTGATGGAACAAAAAGTCGCGGTAATTATGGTGAGTTTAATTTAAAGAGTATTTTGAATGAACATTTATCAAAAGACTTGTGA", "aa": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDL", "aa_hexdigest": "a8011aee3ee43a45f6749d607917feee", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rmuC" ], "truncated": "5-prime", "pseudogene": { "start": 355713, "stop": 356558, "observations": { "mutation": [ 356018 ], "stop": [ 356018 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 300.0, "evalue": 1.42e-98, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.99644128113879, "score": 515.0, "evalue": 1.28e-185, "description": "Internal stop codon at 356018. Nonsense mutation around 356018.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "IGIGPGHMMC_660", "locus": "GCA000148625_00633" }, { "type": "cds", "sequence": "contig_1", "start": 356738, "stop": 356911, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGA", "aa": "MKIVKLTKKEYKGKEFCVQYKTKGYYFIEHTKNGFSFVYKKFDQEKTFGYCDKFFQD", "aa_hexdigest": "e7e1c937be06c6942f6b226f649f2982", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7032.078200000002, "isoelectric_point": 9.369270896911619 }, "id": "IGIGPGHMMC_661", "locus": "GCA000148625_00634" }, { "type": "cds", "sequence": "contig_1", "start": 357068, "stop": 357262, "strand": "+", "frame": 2, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "nt": "ATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAG", "aa": "MKKMIAKAKTTPARMIVLETETCNEKAIRFYQKFNFEVIGFDLYAYTNEDPKEHEVRVEMGLKI", "aa_hexdigest": "05d928382105f62a47e001beb02f031c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1H9U7D2", "query_cov": 0.953125, "subject_cov": 0.8356164383561644, "identity": 0.557, "score": 78.2, "evalue": 9.01e-18, "valid": false, "product": "Acetyltransferase (GNAT) family protein", "uniref50_id": "UniRef50_A0A358PH68" }, "pscc": { "uniref50_id": "UniRef50_A0A358PH68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_662", "locus": "GCA000148625_00635" }, { "type": "cds", "sequence": "contig_1", "start": 357306, "stop": 357590, "strand": "+", "frame": 3, "gene": null, "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0", "UniRef:UniRef90_C4XES0" ], "nt": "ATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGA", "aa": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDK", "aa_hexdigest": "3013309bd069b81796dec4c8369696a4", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 357306, "stop": 357845, "observations": { "mutation": [ 357588 ], "stop": [ 357588 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES0", "query_cov": 1.0, "subject_cov": 0.5251396648044693, "identity": 1.0, "score": 191.0, "evalue": 1.25e-60, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDKWVCAICDAPNVLYENHFIDDKVKYSSFPIIPLVGGKNRNEKMVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA" }, "paralog": false, "identity": 0.994413407821229, "score": 360.0, "evalue": 7.35e-130, "description": "Internal stop codon at 357588. Nonsense mutation around 357588.", "psc": { "uniref90_id": "UniRef90_C4XES0", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES0", "UniRef:UniRef50_A0A3P8MDU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0" ], "product": "Putative intracellular protease/amidase (Putative glutaminase)" } }, "id": "IGIGPGHMMC_663", "locus": "GCA000148625_00636" }, { "type": "cds", "sequence": "contig_1", "start": 357711, "stop": 357845, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAA", "aa": "MVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA", "aa_hexdigest": "db2917e9cc46a20c64f75ee3c74e2c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4858.635200000001, "isoelectric_point": 6.068665122985839 }, "id": "IGIGPGHMMC_664", "locus": "GCA000148625_00637" }, { "type": "cds", "sequence": "contig_1", "start": 358139, "stop": 358300, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTATACAAATCGAAACAACTTACTTTCATTCAAATACAATCAATTAGCTGAAAGAGTTGGCAAGATTAAAATTAGCAAAAAATCATACAAATATTCTTTTGATAAACTAGAAAATTTAATTAAATATTTCTCATTAAATTACAAAAGATGA", "aa": "MLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKR", "aa_hexdigest": "eb7899aac6b2d12912ba15f767e07369", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4464, "isoelectric_point": 10.054183006286618 }, "id": "IGIGPGHMMC_665", "locus": "GCA000148625_00638" }, { "type": "cds", "sequence": "contig_1", "start": 358712, "stop": 359416, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1", "UniRef:UniRef90_C4XES1" ], "nt": "ATGCAAACAAATAATCAACAATTAAAAGAGCAAATTATCGTTTCAAACGATAATCTTTTAATTCGCAATGATACCTTTTTATTTAAAACAAATAACAAAGAAAAAATAGAACTCTCATCAATCTTGCGACTATTGCAAGCTATAAATAAAACTATTTTAAAAAGCAATAGTAACATTTTATTAAGCTTTGAAGGTCAAGAATTTTATAATGAAAATATTCAATATTTTAGCTCTTATTTTATCAAAAATAATCATAATGTTTTTAAGTATCACAATAACTTTTCAATTAGTTTGATTTTAGATGAATATGCCTATAAAAAAGCTTCATATGACTTTTTAATCAAATTTAAAAATATTAAAAATGATAACTATATTACAATTCAAATATTTGATAAAAACTTTGAAATTTTGACCTCTGAAAACTTACAAAAAATTTATGATTATTATCAATTAAACAAATGGCAAAATAAAGAAATTGTCATCAAAGATCCTAAACTAGTTAATGAATTTGAACTGATTGATGAATTAGCTTCAAAAGAACAAATTCTTAAAGCTTTCGTTAATGTTAAACAAAGATATAAAACATTAAGTTACTTTGCTAGTGACAATAAATTTAGTCAAAGTATTGGTACAGAACTATTAAATAATTATCAAAATAAATACATTACAAACAATAAAAATATTTCGCTTTTAAACCTAAAATGA", "aa": "MQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLK", "aa_hexdigest": "8537e5c8cd94727749f5b0d6e3142810", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 358409, "stop": 359431, "observations": { "mutation": [ 358597, 358687, 358714 ], "stop": [ 358597, 358687, 358714 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES1", "query_cov": 1.0, "subject_cov": 0.45525291828793774, "identity": 1.0, "score": 446.0, "evalue": 5.89e-154, "gene_start": 1, "gene_end": 234, "reference_start": 6, "reference_end": 239, "reference_sequence": "MIGNFMQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLKWLTFFAASSWYRENKIQNIFHFDINSNLNVCLRLNDKFKWLNSHELVLIYLDFFLEEIKRNGIDISNSYVVVPQNTTYQILELLKQYKVKYYFYDLKNINKQLKNESCLFAYIDNKFIANPRYSTQFNNYYFFICLIWMLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKRWFTAFNAYRVWNDHKYMLFKLINNKKHQMIFYWDDVKGKLVIEYQMCCNFENISNTVWWDKLKLQILLFVLIKKAQKHKIEKEE" }, "paralog": false, "identity": 0.9882352941176471, "score": 664.0, "evalue": 5.19e-242, "description": "Internal stop codon at 358597, 358687, 358714. Nonsense mutation around 358597, 358687, 358714.", "psc": { "uniref90_id": "UniRef90_C4XES1", "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES1", "UniRef:UniRef50_C4XES1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 27937.44870000004, "isoelectric_point": 9.023269081115721 }, "id": "IGIGPGHMMC_666", "locus": "GCA000148625_00639" }, { "type": "cds", "sequence": "contig_1", "start": 359451, "stop": 362231, "strand": "-", "frame": 3, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "RefSeq:WP_013354563.1", "SO:0001217", "UniParc:UPI0001E330BB", "UniRef:UniRef100_C4XES2", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_A0A3P8MFB5" ], "nt": "ATGAGTTCAAAAGAACAAGATAAAGAAAAAGAATACAAAAGCAAACTTAATCAAGAAGAAGAAAAAGAAAATGCAATCGAAGAATTAGATGAAGATTACATTCCTGATGAAGAGCTTTTTGTTGCTTTTAAACCACAAAAAGAAGAAACTAAAGTTATTGAAGGGGAGGAAGAAGAAGTTCCTCAAAATAAAGACAACTATGTAGTTCAACCACAACTTTTAGATGCACCTAAAGATGGTATTCATCCAGTTGAAGTTCACAAAGAAATGAAAAACTCATTCTTAGAATATGCAATGAGTGTTATTGTTTCTCGTGCTTTACCAGATGCTCGTGATGGACTTAAACCAGTACATAGACGTATTCTTTTTGATATGAATGAATTAGGAATTACATTTGGATCGCAACATAGAAAAAGCGCTCGTATTGTCGGGGACGTTTTAGGTAAGTACCACCCACATGGCGACAGTTCAGTTTATGAAGCTATGGTTCGTATGGCGCAAGATTTTAGTATGCGTTATCCTTTAGTTGATGGTCACGGTAACTTTGGATCTATTGATGGTGATGAAGCTGCTGCGATGCGTTATACTGAAGCAAGAATGAGCAAATTAGCTGCTGAAATGCTTGAAGGTATTAAAAAAGATACAGTAGATTTTGTTGATAACTATGATGCTAGTGAAAAAGAACCTTCAGTATTACCATCAAGATTCCCTAACCTTTTAGTTTCAGGTGGTAGTGGTATTGCTGTTGGTATGGCAACAAGTATTCCACCTCACAACTTAGGTGAAACTATTGATGCAACTATTGCTTTAGCTAACAATCCAGAAATTGATATTTATGGCTTAATGGAATTTTTACCTGGTCCAGACTTTCCTACTGGAGCTATGATTTTAGGCAATGCAGGTATTAAAGATGCATACTTAACAGGCAAGGGATCCATTCCAATCAGATCAATAGCTAAAATAGAAGAATACTCAAATGGTAAAAGCAAAATTATTGTTACTGAAATTCCTTATGAAGTTAAGAAAACTGCAATTATTGAAAAAATAGCTGAATTAGTTAAAGATAAACAAATCGAAGGTATCGCCGACTTACGTGATGAAAGTAGCCGTGAAGGTATTAGAATTGTTATTGACGTTAAAAAGAATGTTAATCCTCATGTTTTATTAAACAAGCTTTATCGTCAAACAAACTTACAAGTAAACTACAATGTTAACTTTGTTGCTTTAGTTGATGGTGAACCTAAAGTTTTAAACTTAAAACAAGCTTTAGAAGTTTACTTAAGACACCAAGAAGAAGTAGTGACTAGAAGATTAAAATTTGATTTAATTAAAGCTCAAGACCGTCTTCACATTTTAGAAGGTCTTAAAATTGCAGTTGAAAATATTGATGAAGTTATTGCAATTATTAAGAAATCTAAAACAGATGGTGAAGCTCAAGCTAACTTAGCAAAAAGATTTAATTTAACTGAAAAACAAACAAAAGCTATTGTTGACATGCGTTTAGGTCGTCTTACAGGACTTGCTATTGAAAACATGCTTGAAGAAATGGCAGAGTTAAGAAAAGAAATTGAAAGAATTGAATTTATTTTGGCTAACCATAGTGAATTAATTAAATTAATTGTTAAAGAATTAACTGAAATTAAAGAAAAATACAATGACAAACGTCGCACACAAATTAACTATGAAATTCAAGGTACTATTACTGATGAAGACTTGATTCCTCAACGTGACATTGTTATTACAACCAGCGCCAAAGGCTTTGTAAAAAGAATGGACTTAGAAGAATACAAAACTCAACGTCGTGGTGGAGTTGGTATTGGTACTATGAAAACATATGACGATGATGATATTGCCTCAATATTAACAACTACAACTCACACCGACTTACTATTATTCTCAAATCATGCTCGTGTCTACCGTGTTAGAGCTCATGAAATTCCAGAATTATCTCGTCAAAGTAAAGGTGTAGCATTCATTAATATTATTCCTTCACTTCGTGTTAAAGAAGGTGAAAGAATTGTTTCAATGCTTCCAGTAGACACTTACGATGAAGATCACTACTTATTTACAGCAACTAAAAATGGTATTATCAAGAAAACTAGATTAACTGAATACGAAAGAATTAACGCTAATGGTAAATATGCTTTCAACTTACGTGAAGGCGATGAATTAATTAGAGCAATGATTATTACTGATGAAGACTTAATTCTTTTAGCAAATGATGAAGAAAGAATTGTTAAATTCCAAGCTATTGACTTTAGACCAATTAGTAGAACTGCAACTGGTGTTAAAGGTATAACACTTGATAGCCATCAAACAGTTTCAAGCACTTCAACAAGTCAAGAAGGTGAATTAATCTTAACTATTGGTAAAAAAGGTTTTGGAAAACTTACTCACCACTCACTATTTAGATTAATTAAACGTGGTGGTAAAGGTGTTAAAGGAATTAATAGCAAATTAGCAGGCGACATTGTTTTTGCTAGATTCGTAAATCCTCAAGATGAACTTTTAATTATTACAACAAGTGGTTTAACAATTAGAACAGCTATTAGCCAAATTAGCCAAACTGGTCGGGCAGCTAAAGGTGTTAAAATAATTAACCTTAAAAAGCGTGAAGAAATTCAAGCTGTTGAAATCATTAAAATTGATGAAAAAACAGATCCAGAAGCAACTATGGCTGCAACTTCTGTTGTTCAAAGCATTTATGAACAAAGTCAAGAAATCAACTTAGATGATAACTCAAACGATGAAATTGATATTGATTCAAACAATGAAGAATAA", "aa": "MSSKEQDKEKEYKSKLNQEEEKENAIEELDEDYIPDEELFVAFKPQKEETKVIEGEEEEVPQNKDNYVVQPQLLDAPKDGIHPVEVHKEMKNSFLEYAMSVIVSRALPDARDGLKPVHRRILFDMNELGITFGSQHRKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSMRYPLVDGHGNFGSIDGDEAAAMRYTEARMSKLAAEMLEGIKKDTVDFVDNYDASEKEPSVLPSRFPNLLVSGGSGIAVGMATSIPPHNLGETIDATIALANNPEIDIYGLMEFLPGPDFPTGAMILGNAGIKDAYLTGKGSIPIRSIAKIEEYSNGKSKIIVTEIPYEVKKTAIIEKIAELVKDKQIEGIADLRDESSREGIRIVIDVKKNVNPHVLLNKLYRQTNLQVNYNVNFVALVDGEPKVLNLKQALEVYLRHQEEVVTRRLKFDLIKAQDRLHILEGLKIAVENIDEVIAIIKKSKTDGEAQANLAKRFNLTEKQTKAIVDMRLGRLTGLAIENMLEEMAELRKEIERIEFILANHSELIKLIVKELTEIKEKYNDKRRTQINYEIQGTITDEDLIPQRDIVITTSAKGFVKRMDLEEYKTQRRGGVGIGTMKTYDDDDIASILTTTTHTDLLLFSNHARVYRVRAHEIPELSRQSKGVAFINIIPSLRVKEGERIVSMLPVDTYDEDHYLFTATKNGIIKKTRLTEYERINANGKYAFNLREGDELIRAMIITDEDLILLANDEERIVKFQAIDFRPISRTATGVKGITLDSHQTVSSTSTSQEGELILTIGKKGFGKLTHHSLFRLIKRGGKGVKGINSKLAGDIVFARFVNPQDELLIITTSGLTIRTAISQISQTGRAAKGVKIINLKKREEIQAVEIIKIDEKTDPEATMAATSVVQSIYEQSQEINLDDNSNDEIDIDSNNEE", "aa_hexdigest": "c1907b31a1ae232dcb9284fadec2ec78", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E330BB", "ncbi_nrp_id": "WP_013354563.1", "uniref100_id": "UniRef100_C4XES2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330BB", "RefSeq:WP_013354563.1", "UniRef:UniRef100_C4XES2" ] }, "ips": { "uniref100_id": "UniRef100_C4XES2", "uniref90_id": "UniRef90_A0A3P8MFB5" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MFB5", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "IGIGPGHMMC_667", "locus": "GCA000148625_00640" }, { "type": "cds", "sequence": "contig_1", "start": 362649, "stop": 362753, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGA", "aa": "MTINRMITKFDEENPGILVKMQKAHIAPSFLAYI", "aa_hexdigest": "52ceff1930d05be9e6b2bd35a8ec370e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3921.6504999999997, "isoelectric_point": 8.211289787292483 }, "id": "IGIGPGHMMC_668", "locus": "GCA000148625_00641" }, { "type": "cds", "sequence": "contig_1", "start": 362894, "stop": 363052, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACATAACTTTTTTATCAAGTGTAATATTTTTACGATTTAAAATCAATATGACTATTCCAATTAATGGATTAATTGCATGTACAATTGTGCTTAAAGCTAATCTAAAGGAACCATACTCTGATCCAAATATTTTTGGAAAAATTAAAGTTCAATAA", "aa": "MYITFLSSVIFLRFKINMTIPINGLIACTIVLKANLKEPYSDPNIFGKIKVQ", "aa_hexdigest": "99409788cfdf9cb41ca922d09144a65b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.127000000001, "isoelectric_point": 9.655059242248537 }, "id": "IGIGPGHMMC_669", "locus": "GCA000148625_00642" }, { "type": "cds", "sequence": "contig_1", "start": 363600, "stop": 364091, "strand": "+", "frame": 3, "gene": null, "product": "Small ribosomal subunit protein uS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "nt": "ATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGA", "aa": "MRYRGPVFKKSRRYGFSILETGKEFSKGRKRTYAPGQHGDKRVKLSDYGLHLYEKQKVKYLYGLNEKQMHKYYIRATKIKGVTGTVLLQLLESRLDNLVYRAGFASTRRQARQLVSHGHFTLNGHKADIPSMNVEVGSKIQLKEKSQKNKIIEANLKDRSAAK", "aa_hexdigest": "fee83a092c3b0cd7e14f7ad20f689366", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A974WKF8", "query_cov": 1.0, "subject_cov": 0.8232323232323232, "identity": 0.816, "score": 258.0, "evalue": 8.85e-86, "valid": false, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [], "id": "IGIGPGHMMC_670", "locus": "GCA000148625_00643" }, { "type": "cds", "sequence": "contig_1", "start": 364216, "stop": 364455, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354566.1", "SO:0001217", "UniParc:UPI0001A8C219", "UniRef:UniRef100_C4XES6", "UniRef:UniRef50_A0A449BAA4", "UniRef:UniRef90_A0A3P8KXC8" ], "nt": "ATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAA", "aa": "MKKNIHPQYFTVEATCSTCGKKFQFGSTKKSISVDVCSGCHVVYTGDRTKTKATGMIDKFNQRLAKQKAAIEKSKGKKK", "aa_hexdigest": "a74ba465a9352977cd327abffc417088", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C219", "ncbi_nrp_id": "WP_013354566.1", "uniref100_id": "UniRef100_C4XES6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C219", "RefSeq:WP_013354566.1", "UniRef:UniRef100_C4XES6" ] }, "ips": { "uniref100_id": "UniRef100_C4XES6", "uniref90_id": "UniRef90_A0A3P8KXC8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KXC8", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_A0A449BAA4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAA4" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "IGIGPGHMMC_671", "locus": "GCA000148625_00644" }, { "type": "cds", "sequence": "contig_1", "start": 364642, "stop": 365436, "strand": "+", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "GO:0003677", "GO:0006355", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_N9VB78", "UniRef:UniRef90_C4XES7" ], "nt": "ATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGA", "aa": "MANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSI", "aa_hexdigest": "78cd355c64ac915be62402a5bae62818", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "3-prime", "pseudogene": { "start": 364639, "stop": 365664, "observations": { "mutation": [ 365434 ], "stop": [ 365434 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES7", "query_cov": 1.0, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 488.0, "evalue": 9.56e-173, "gene_start": 1, "gene_end": 264, "reference_start": 2, "reference_end": 265, "reference_sequence": "MMANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSIWQTIESEIDEDENIKIAICDDHSTFISKKLETQSKIKEIALVGTSRMDYEKGFSALKALEEFIENKPCKSKDEGDK" }, "paralog": false, "identity": 0.9941348973607038, "score": 647.0, "evalue": 1e-237, "description": "Internal stop codon at 365434. Nonsense mutation around 365434.", "psc": { "uniref90_id": "UniRef90_C4XES7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_N9VB78", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES7", "UniRef:UniRef50_N9VB78", "KEGG:K03705", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_N9VB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VB78" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "IGIGPGHMMC_672", "locus": "GCA000148625_00645" }, { "type": "cds", "sequence": "contig_1", "start": 365572, "stop": 365664, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAA", "aa": "MDYEKGFSALKALEEFIENKPCKSKDEGDK", "aa_hexdigest": "c21a28a2550c58b0a55b5ed381902f00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3450.8446000000013, "isoelectric_point": 4.812637138366699 }, "id": "IGIGPGHMMC_673", "locus": "GCA000148625_00646" }, { "type": "cds", "sequence": "contig_1", "start": 365664, "stop": 366917, "strand": "+", "frame": 3, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "KEGG:K03687", "RefSeq:WP_013354568.1", "SO:0001217", "UniParc:UPI0001E330BF", "UniRef:UniRef100_A0AB32XBD6", "UniRef:UniRef50_C4XES8", "UniRef:UniRef90_C4XES8" ], "nt": "ATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACGGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAA", "aa": "MKVVPNLNKFKTGEGIIANISVYNSSNAYQKQLSKEKVSFVLGENTFLPNFDNNIVEQTVRPVLDFVVTFPKSYEVAEFKDKTFRFVIEILDYQDSEYVKAYKQMLALREEIQKLNVASGNHLKELEELKAKNEKQMSEISNLKTENDKHTKDSADLKNQYQEQEKTIQKLIEDNKKFIADNNALKEDNENKGKQIKELMSGKTTKLMEQTSEHEAKGVNPAVLRKQVDVLRKQLEEQSIELKELRKNKAPEAVQRVTVPKEEVDKIKQYALQKFFEDFSTHYTTLKGAVKAGAKSENSSVKNYVVGFEMILNLINGVLEKHGIKAIEPKIGDEFDPNTQKVLEVEECKDKKHNTIVKVSAIGFKLHDRVIKPALVVIAQDSSAKDKKLAAETKKPEAPKAKETTKKTETKKTTTKK", "aa_hexdigest": "a78c5899fed54e642ee4f195fc36391c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330BF", "ncbi_nrp_id": "WP_013354568.1", "uniref100_id": "UniRef100_A0AB32XBD6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330BF", "RefSeq:WP_013354568.1", "UniRef:UniRef100_A0AB32XBD6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBD6", "uniref90_id": "UniRef90_C4XES8" }, "psc": { "uniref90_id": "UniRef90_C4XES8", "product": "Protein GrpE", "uniref50_id": "UniRef50_C4XES8", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_C4XES8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES8" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "IGIGPGHMMC_674", "locus": "GCA000148625_00647" }, { "type": "cds", "sequence": "contig_1", "start": 367137, "stop": 367505, "strand": "+", "frame": 3, "gene": null, "product": "Large conductance mechanosensitive channel", "db_xrefs": [ "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_A0A077LBI7", "UniRef:UniRef90_C4XES9" ], "nt": "ATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAA", "aa": "MFMLAIGLLLGTAFNAVVSSLANDVIMAAIAKAFNVDEVKDLKAGPILIGKFLAALISFLIVATVIFILLVVVFLVKNAIETRKFKKNPPVAEEPKPTTEELILAEIKKLNERLEKLEQPKK", "aa_hexdigest": "4071d75cdfd5135218d201d4f67d5683", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XES9", "query_cov": 1.0, "subject_cov": 0.8776978417266187, "identity": 1.0, "score": 209.0, "evalue": 1.06e-67, "valid": true, "product": "Large conductance mechanosensitive channel", "uniref50_id": "UniRef50_A0A077LBI7", "kegg_orthology_id": "K03282" }, "pscc": { "uniref50_id": "UniRef50_A0A077LBI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBI7" ], "product": "Large-conductance mechanosensitive channel" }, "genes": [], "id": "IGIGPGHMMC_675", "locus": "GCA000148625_00648" }, { "type": "cds", "sequence": "contig_1", "start": 367618, "stop": 367725, "strand": "+", "frame": 1, "gene": null, "product": "HTH-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1", "UniRef:UniRef90_A0AB32XCG9" ], "nt": "ATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAA", "aa": "MVIFDLFNYNKGLYGYRRITFVLRNKEVMINYKKD", "aa_hexdigest": "09eee22433129b8a8b122a6da5976d4e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 367621, "stop": 367769, "observations": { "deletion": [ 367723 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCG9", "query_cov": 0.9428571428571428, "subject_cov": 0.6111111111111112, "identity": 0.848, "score": 63.9, "evalue": 7.18e-13, "gene_start": 2, "gene_end": 34, "reference_start": 4, "reference_end": 36, "reference_sequence": "MKKIIFDLFNYNKGLYGYRRITFALRNKGIMINHKKVQKLIKSLNLFGKTLRKK" }, "paralog": false, "identity": 0.8235294117647058, "score": 80.1, "evalue": 3.95e-22, "description": "Frameshift due to deletion around 367723.", "psc": { "uniref90_id": "UniRef90_A0AB32XCG9", "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCG9", "UniRef:UniRef50_C4XEC1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1" ], "product": "HTH-like domain-containing protein" } }, "id": "IGIGPGHMMC_676", "locus": "GCA000148625_00649" }, { "type": "cds", "sequence": "contig_1", "start": 367957, "stop": 368097, "strand": "+", "frame": 1, "gene": null, "product": "Integrase catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0", "UniRef:UniRef90_C4XET0" ], "nt": "ATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGA", "aa": "MNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQG", "aa_hexdigest": "8f2563d043fc78156bf87b6c69c55c2d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 367804, "stop": 368151, "observations": { "mutation": [ 367894, 368095 ], "stop": [ 367894, 368095 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET0", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 94.0, "evalue": 7.5e-24, "gene_start": 1, "gene_end": 46, "reference_start": 52, "reference_end": 97, "reference_sequence": "MPNLLLDKENITEDFFRYKRNFSNEKYLDIWGTDVTEFKLINEEKIYFSPVMNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQGWQYTHQDYIDYLKEKNAI" }, "paralog": false, "identity": 0.9739130434782609, "score": 228.0, "evalue": 4.21e-79, "description": "Internal stop codon at 367894, 368095. Nonsense mutation around 367894, 368095.", "psc": { "uniref90_id": "UniRef90_C4XET0", "product": "Integrase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET0", "UniRef:UniRef50_C4XET0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0" ], "product": "Integrase catalytic domain-containing protein" } }, "id": "IGIGPGHMMC_677", "locus": "GCA000148625_00650" }, { "type": "cds", "sequence": "contig_1", "start": 368497, "stop": 368694, "strand": "+", "frame": 1, "gene": null, "product": "Class IIb bacteriocin, lactobin A/cerein 7B family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6", "UniRef:UniRef90_C4XET1" ], "nt": "ATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGA", "aa": "MKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIK", "aa_hexdigest": "2b2fb9a0f6957ba4c47116ceac72589a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 368458, "stop": 368736, "observations": { "mutation": [ 368692 ], "stop": [ 368692 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET1", "query_cov": 1.0, "subject_cov": 0.6989247311827957, "identity": 1.0, "score": 127.0, "evalue": 4.98e-37, "gene_start": 1, "gene_end": 65, "reference_start": 14, "reference_end": 78, "reference_sequence": "MLPKRKVKNERRTMKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIKWDNSPATNGNIFYAW" }, "paralog": false, "identity": 0.9782608695652174, "score": 178.0, "evalue": 1.05e-59, "description": "Internal stop codon at 368692. Nonsense mutation around 368692.", "psc": { "uniref90_id": "UniRef90_C4XET1", "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET1", "UniRef:UniRef50_A0A3P8MFB6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6" ], "product": "Class IIb bacteriocin, lactobin A/cerein 7B family" } }, "id": "IGIGPGHMMC_678", "locus": "GCA000148625_00651" }, { "type": "cds", "sequence": "contig_1", "start": 369016, "stop": 369636, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2", "UniRef:UniRef50_Q05426", "UniRef:UniRef90_Q05426" ], "nt": "ATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAA", "aa": "MKKPTAEHMINENIPFQKVFVVGANGEKVGVLSTREAIEMAKEQRLDLVLISVQPKPIARILDYGKFKYERKKKQKAAKEKQTVIQNRQIRLTPLIGEHDLNVKARKAKEFLLDGDRIKVSLKFRGRESARPELGHKTLEKFYKAVEDLADIAKEAELVNDRFLDMYLQPNKIKVNKYRKEHNLVDKNSDSQDKCASEEDTNEGEQ", "aa_hexdigest": "a0f29309140a45a2b7ddf14c8c28bc66", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E330C1", "uniref100_id": "UniRef100_C4XET2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2" ] }, "ips": { "uniref100_id": "UniRef100_C4XET2", "uniref90_id": "UniRef90_Q05426" }, "psc": { "uniref90_id": "UniRef90_Q05426", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_Q05426", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q05426", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q05426" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "IGIGPGHMMC_679", "locus": "GCA000148625_00652" }, { "type": "cds", "sequence": "contig_1", "start": 369638, "stop": 369826, "strand": "+", "frame": 2, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_013526795.1", "SO:0001217", "UniParc:UPI000016F938", "UniRef:UniRef100_Q05428", "UniRef:UniRef50_Q05428", "UniRef:UniRef90_Q05428" ], "nt": "ATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAA", "aa": "MPKMKTKSALKKRIKITGTGKIMREQAYRSHLSQNKTTKQKRQARKSVQMHSSDVKRFKALI", "aa_hexdigest": "cdc21f893c75ed1d684dbb06b25c3927", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000016F938", "ncbi_nrp_id": "WP_013526795.1", "uniref100_id": "UniRef100_Q05428", "db_xrefs": [ "SO:0001217", "UniParc:UPI000016F938", "RefSeq:WP_013526795.1", "UniRef:UniRef100_Q05428" ] }, "ips": { "uniref100_id": "UniRef100_Q05428", "uniref90_id": "UniRef90_Q05428" }, "psc": { "uniref90_id": "UniRef90_Q05428", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_Q05428", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmI" ], "id": "IGIGPGHMMC_680", "locus": "GCA000148625_00653" }, { "type": "cds", "sequence": "contig_1", "start": 370211, "stop": 370516, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A622", "UniRef:UniRef100_A0AB32XBD7", "UniRef:UniRef50_C4XET5", "UniRef:UniRef90_C4XET5" ], "nt": "ATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCCCAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAA", "aa": "MQNNNIKILFKMNPKFRTFGKGSFLYNLNLENKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALLQLKDSLTKKNSK", "aa_hexdigest": "d5df08b61ab78c2622bc9f902d821f96", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A622", "uniref100_id": "UniRef100_A0AB32XBD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A622", "UniRef:UniRef100_A0AB32XBD7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBD7", "uniref90_id": "UniRef90_C4XET5" }, "psc": { "uniref90_id": "UniRef90_C4XET5", "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_681", "locus": "GCA000148625_00654" }, { "type": "cds", "sequence": "contig_1", "start": 370582, "stop": 371271, "strand": "+", "frame": 1, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFF0", "UniRef:UniRef90_C4XET6" ], "nt": "TTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGA", "aa": "MKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARL", "aa_hexdigest": "6c839917bf40975022c366e9fab0935c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 370513, "stop": 371514, "observations": { "mutation": [ 371269, 371449 ], "stop": [ 371269, 371449 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET6", "query_cov": 1.0, "subject_cov": 0.6815476190476191, "identity": 0.996, "score": 446.0, "evalue": 7.69e-157, "gene_start": 1, "gene_end": 229, "reference_start": 24, "reference_end": 252, "reference_sequence": "MTIELKQEFKKLFSIKSILEKIKLKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARLWQERKLETYYPIIYIDAVHFHIVDNNVVTKKATYVIMGINGDGQKEILGLYIRENESAKFWMSVLNALKNRGISKIDIICSQII" }, "paralog": false, "identity": 0.978978978978979, "score": 644.0, "evalue": 2.5e-237, "description": "Internal stop codon at 371269, 371449. Nonsense mutation around 371269, 371449.", "psc": { "uniref90_id": "UniRef90_C4XET6", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFF0", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET6", "UniRef:UniRef50_C4XFF0", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF0" ], "product": "Mutator family transposase" } }, "id": "IGIGPGHMMC_682", "locus": "GCA000148625_00655" }, { "type": "cds", "sequence": "contig_1", "start": 371452, "stop": 371718, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "b1d2697471a467a119612aa745a6f757", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 7.451184768013668e-09, "score": 32.01397705078125, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10129.612099999993, "isoelectric_point": 8.806848716735843 }, "id": "IGIGPGHMMC_683", "locus": "GCA000148625_00656" }, { "type": "cds", "sequence": "contig_1", "start": 372430, "stop": 372525, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGATCATGCTTCAACTACTTTCTTATTAAAACATAATGGTGAACCTATTTCATGA", "aa": "MPFSNTLFSYLRTVDHASTTFLLKHNGEPIS", "aa_hexdigest": "926f94348d4dc2b9400c4f72fc0640c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3524.9525000000003, "isoelectric_point": 6.694576072692871 }, "id": "IGIGPGHMMC_684", "locus": "GCA000148625_00657" }, { "type": "cds", "sequence": "contig_1", "start": 372742, "stop": 372906, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAACATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAAGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCCTTCGATTTCATTAAATACAATAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKTSDSKIMFKNDFQSNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "dbccbc6003fce992d206baffb5cabe3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6355.1217000000015, "isoelectric_point": 8.963700294494632 }, "id": "IGIGPGHMMC_685", "locus": "GCA000148625_00658" }, { "type": "cds", "sequence": "contig_1", "start": 373387, "stop": 373605, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTGCAAAAATTAATTCAATTAAAAATTATTTAGAAAATGATTATTTATTAGCCGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFAKINSIKNYLENDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "be262ada710dde32b74c0c884593f0b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8388.426699999996, "isoelectric_point": 4.867032051086425 }, "id": "IGIGPGHMMC_686", "locus": "GCA000148625_00659" }, { "type": "cds", "sequence": "contig_1", "start": 373717, "stop": 373884, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAATTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGTTATTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEIYKNVNSTENFSVIQADKDNKVNFVKK", "aa_hexdigest": "cde530d978da5e11e1277961cd9f5198", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.042100000002, "isoelectric_point": 7.942263221740723 }, "id": "IGIGPGHMMC_687", "locus": "GCA000148625_00660" }, { "type": "cds", "sequence": "contig_1", "start": 374614, "stop": 375246, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGTGGTGTTTTAGTAAATAGAAGTGTTATTATAAAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACAAAGGAATATATAAATCGTTATGTTAACACTTTTGTCAAAACAATTGAAAGTACAAAATTATTTAACAAATCTCAAATTTTAGACTTAAGCAGCAAATCATTAACGCCTATTGTTAATGCTCTTAATGAATTTAACTCATTTATTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAATTTATTCTAAACGCTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATATTTCAAATGTTGATTCAATTGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATCAATTTGTACAAAAATCTAAAATTGAATTGAGTTCTTTTAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIKDIIKACKTLEQSYGNTKEYINRYVNTFVKTIESTKLFNKSQILDLSSKSLTPIVNALNEFNSFIESSNQISFEKLDEFINKIQFILNAENDLTTSIQSVIKEFLNAYISNVDSIETTIRLAKLNNSKQIIDQFVQKSKIELSSFK", "aa_hexdigest": "c7c3d12a71f10ae70ecb89e6ee1af0d3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 374416, "stop": 375246, "observations": { "mutation": [ 374616 ], "stop": [ 374616 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.919, "score": 367.0, "evalue": 1.41e-126, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.8876811594202898, "score": 470.0, "evalue": 1.16e-169, "description": "Internal stop codon at 374616. Nonsense mutation around 374616.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23753.78520000002, "isoelectric_point": 5.68954906463623 }, "id": "IGIGPGHMMC_688", "locus": "GCA000148625_00661" }, { "type": "cds", "sequence": "contig_1", "start": 375301, "stop": 375765, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATCTTGGTCACCACTTTGGTAACAAAAGTTATATGAGAACTGTTAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHLGHHFGNKSYMRTVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHV", "aa_hexdigest": "17efb620cb5c875ee72ec80fcc4d0074", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 375265, "stop": 376026, "observations": { "mutation": [ 375303, 375780, 375795, 375819, 375897 ], "stop": [ 375303, 375780, 375795, 375819, 375897 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.968, "score": 280.0, "evalue": 1.78e-93, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9209486166007905, "score": 441.0, "evalue": 1.7e-159, "description": "Internal stop codon at 375303, 375780, 375795, 375819, 375897. Nonsense mutation around 375303, 375780, 375795, 375819, 375897.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17770.864899999997, "isoelectric_point": 6.302102851867676 }, "id": "IGIGPGHMMC_689", "locus": "GCA000148625_00662" }, { "type": "cds", "sequence": "contig_1", "start": 375895, "stop": 376026, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGGATTGTTGGTGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAGTTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "IGIGPGHMMC_690", "locus": "GCA000148625_00663" }, { "type": "cds", "sequence": "contig_1", "start": 376332, "stop": 376778, "strand": "-", "frame": 3, "gene": null, "product": "site-specific integrase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE838E", "UniRef:UniRef90_UPI0002EE838E" ], "nt": "ATGCCTTTAATAGGTAATCTTAGAGAAGATATATTAGAAAATATAGATAAATTACGTATTTCTGAAAAATCAATTAAAAATATGTTTTATCTTTTTAAAAAACATATTAGTGAAAAATATCCTGATTTTAAATATAAAATCTCGGCTCACGTCTTAAGACATAATTATGCAACTAAAGCTTTTTATAATTTAAAAAATAATGATTTAGTAGCCAAATTATTAGGACACACAAATTCAAATATTACTCAAAAAACTTATATTGATTATGATAAAAGTATCATGGATGAGTATGCTATTTTAGCCATACAAGATCAAAAAATAGGCTTAAATTCTATGCAATTATTAAATATAAATAATAATTTAGAGCTTACAATTATCAAATTAGAGAAAGAAAATTGCAGCTTAAAAGAAGAAATTGAGATGCTAAAAAATAAAATAAAAAACTAA", "aa": "MPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKN", "aa_hexdigest": "b998aca4c2fad4c53f564ac6868d62a5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 376332, "stop": 377078, "observations": { "mutation": [ 376847 ], "stop": [ 376847 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002EE838E", "query_cov": 1.0, "subject_cov": 0.5323741007194245, "identity": 1.0, "score": 285.0, "evalue": 1.57e-95, "gene_start": 1, "gene_end": 148, "reference_start": 131, "reference_end": 278, "reference_sequence": "MNRELANNKISNNTKLTKRNIFSSFLNWFCKITNTNMDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKENWSFIIKTLKNNNPRYFRIPSKLEMPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKN" }, "paralog": false, "identity": 0.9959677419354839, "score": 485.0, "evalue": 9.75e-177, "description": "Internal stop codon at 376847. Nonsense mutation around 376847.", "psc": { "uniref90_id": "UniRef90_UPI0002EE838E", "product": "site-specific integrase", "uniref50_id": "UniRef50_UPI0002EE838E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002EE838E", "UniRef:UniRef50_UPI0002EE838E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002EE838E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE838E" ], "product": "site-specific integrase" } }, "id": "IGIGPGHMMC_691", "locus": "GCA000148625_00664" }, { "type": "cds", "sequence": "contig_1", "start": 376845, "stop": 377060, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGGCAAAAGACAAGCTATACCTGAAAAGGAATTAATTAAACTTGTTGAAGAATTAAAAAGCTACAAAAACACCAAATTTAGTATCATTTTTCGTATTTTTCTTTCAACTGGTATTAGAGTTGGAGAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAGAGAATTGA", "aa": "MDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKEN", "aa_hexdigest": "c32fee22b7da91596b9956dae8297d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8507.892799999994, "isoelectric_point": 9.155622673034667 }, "id": "IGIGPGHMMC_692", "locus": "GCA000148625_00665" }, { "type": "cds", "sequence": "contig_1", "start": 377428, "stop": 377559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "IGIGPGHMMC_693", "locus": "GCA000148625_00666" }, { "type": "cds", "sequence": "contig_1", "start": 377689, "stop": 378153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTADEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "d58bf7ad8ae3df16d904eca596400295", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 377428, "stop": 378189, "observations": { "mutation": [ 377557, 377635, 377659, 377674, 378151 ], "stop": [ 377557, 377635, 377659, 377674, 378151 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.935, "score": 271.0, "evalue": 2.78e-90, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8932806324110671, "score": 432.0, "evalue": 5.35e-156, "description": "Internal stop codon at 377557, 377635, 377659, 377674, 378151. Nonsense mutation around 377557, 377635, 377659, 377674, 378151.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17768.86899999999, "isoelectric_point": 5.962433052062988 }, "id": "IGIGPGHMMC_694", "locus": "GCA000148625_00667" }, { "type": "cds", "sequence": "contig_1", "start": 378208, "stop": 378840, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTAAAATTCTAGACTTAAGCAGCAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAGTTCAAATGTCGATTCAATTGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGACCAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSKILDLSSKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYSSNVDSIETTIRLAKLNNSKQIIDQFVQKSKIELNSLK", "aa_hexdigest": "0e3fdb36a52457dbc1f1dcffdc68328d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 378208, "stop": 379038, "observations": { "mutation": [ 378838 ], "stop": [ 378838 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9570000000000001, "score": 377.0, "evalue": 1.1e-130, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9456521739130435, "score": 503.0, "evalue": 1.37e-182, "description": "Internal stop codon at 378838. Nonsense mutation around 378838.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23703.68530000002, "isoelectric_point": 6.410324287414551 }, "id": "IGIGPGHMMC_695", "locus": "GCA000148625_00668" }, { "type": "cds", "sequence": "contig_1", "start": 379164, "stop": 379478, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAA", "aa": "MIKFIFSNSPLNFSLSFRISLFSIEDISSVDKSGLLLSISSIVFLTSSNWLNVFKLAKTFDKKSTIFGFALLISFEISLGFEAKLLTFCKFKSVAFLIILNISK", "aa_hexdigest": "6036e17d51597afc3f4d971a728c2a35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11676.756399999982, "isoelectric_point": 9.667566108703614 }, "id": "IGIGPGHMMC_696", "locus": "GCA000148625_00669" }, { "type": "cds", "sequence": "contig_1", "start": 379570, "stop": 379737, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCGAAATTAGCAACAATGGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLATMANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "49892cd94114844b2b217e714c8dfe30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.973100000005, "isoelectric_point": 7.942263221740723 }, "id": "IGIGPGHMMC_697", "locus": "GCA000148625_00670" }, { "type": "cds", "sequence": "contig_1", "start": 379849, "stop": 380067, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNNDLIKNYLENDYLSAVKDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "1d4319ff09a22acc5365a504b9799701", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8406.355899999999, "isoelectric_point": 4.6834421157836905 }, "id": "IGIGPGHMMC_698", "locus": "GCA000148625_00671" }, { "type": "cds", "sequence": "contig_1", "start": 380503, "stop": 380709, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "512f6d302678aef6e46dcbb1e9f8c22a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8002.109400000004, "isoelectric_point": 8.830702018737792 }, "id": "IGIGPGHMMC_699", "locus": "GCA000148625_00672" }, { "type": "cds", "sequence": "contig_1", "start": 381631, "stop": 381768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGTTTTATATTCTAGAGGAATGCCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTCGAAGATGTAAGATTATGA", "aa": "MIFVLYSRGMPTRDVSDFMFSKYGVNYSPAQISQLTNEIVEDVRL", "aa_hexdigest": "54078c20f8f59997e722d9839537b3cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5218.9333, "isoelectric_point": 4.8610639572143555 }, "id": "IGIGPGHMMC_700", "locus": "GCA000148625_00673" }, { "type": "cds", "sequence": "contig_1", "start": 381949, "stop": 382215, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCTGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAAGATATGAAAGAATTTTGCAATGATTTAAAATCCGTATATCAATCTAATGATGTAAAAAATGAAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQSIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCNDLKSVYQSNDVKNEMDNLDIFEDK", "aa_hexdigest": "ee8defd96810eaea1c7d7fa5b63de03a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 4.7400733623230563e-08, "score": 29.436288833618164, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10217.630999999996, "isoelectric_point": 7.677094841003419 }, "id": "IGIGPGHMMC_701", "locus": "GCA000148625_00674" }, { "type": "cds", "sequence": "contig_1", "start": 382276, "stop": 382443, "strand": "+", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTATTCTCCTGAAATAAGAAAGCTTATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTTCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSPEIRKLIYTTNPIENLNSVFRKYTKTKRVFPSDDSLLKILFLASQQIIKK", "aa_hexdigest": "2f540b94f388a8d670fa8bdecf620a92", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.611, "score": 71.2, "evalue": 2.54e-15, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "IGIGPGHMMC_702", "locus": "GCA000148625_00675" }, { "type": "cds", "sequence": "contig_1", "start": 383392, "stop": 383598, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAACGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATATAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "84a9c29ce6fbec573d274b5d361b5d54", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8060.188600000002, "isoelectric_point": 8.803109550476076 }, "id": "IGIGPGHMMC_703", "locus": "GCA000148625_00676" }, { "type": "cds", "sequence": "contig_1", "start": 383780, "stop": 384055, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCACTAGTACTATTTCCTTTATCTACTCCATATCGGTGCCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAAACTTTAGGTTTAGCTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAA", "aa": "MISLVLFPLSTPYRCHEYSALTLSPLEFPQPPFVLPFEYPPVSKSEIRSYRCFLPKTLGLAQIFDFSSQFALFCETDVGLYNFVGDQYTSP", "aa_hexdigest": "5c75926541399543c80eda9251aba715", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10410.876099999992, "isoelectric_point": 4.794903373718263 }, "id": "IGIGPGHMMC_704", "locus": "GCA000148625_00677" }, { "type": "cds", "sequence": "contig_1", "start": 384037, "stop": 384255, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNNDLIKNYLENDYLSAVKDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "1d4319ff09a22acc5365a504b9799701", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8406.355899999999, "isoelectric_point": 4.6834421157836905 }, "id": "IGIGPGHMMC_705", "locus": "GCA000148625_00678" }, { "type": "cds", "sequence": "contig_1", "start": 384367, "stop": 384534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCGAAATTAGCAACAATGGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLATMANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "49892cd94114844b2b217e714c8dfe30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.973100000005, "isoelectric_point": 7.942263221740723 }, "id": "IGIGPGHMMC_706", "locus": "GCA000148625_00679" }, { "type": "cds", "sequence": "contig_1", "start": 384626, "stop": 384940, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAA", "aa": "MIKFIFSNSPLNFSLSFRISLFSIEDISSVDKSGLLLSISSIVFLTSSNWLNVFKLAKTFDKKSTIFGFALLISFEISLGFEAKLLTFCKFKSVAFLIILNISK", "aa_hexdigest": "6036e17d51597afc3f4d971a728c2a35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11676.756399999982, "isoelectric_point": 9.667566108703614 }, "id": "IGIGPGHMMC_707", "locus": "GCA000148625_00680" }, { "type": "cds", "sequence": "contig_1", "start": 385264, "stop": 385896, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTAAAATTCTAGACTTAAGCAGCAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAGTTCAAATGTCGATTTAATTGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGACCAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSKILDLSSKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYSSNVDLIETTIRLAKLNNSKQIIDQFVQKSKIELNSLK", "aa_hexdigest": "aeb87ff0ac053d71f727987371b55037", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 385066, "stop": 385896, "observations": { "mutation": [ 385185, 385266 ], "stop": [ 385185, 385266 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9520000000000001, "score": 377.0, "evalue": 1.56e-130, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9347826086956522, "score": 495.0, "evalue": 1.07e-179, "description": "Internal stop codon at 385185, 385266. Nonsense mutation around 385185, 385266.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23729.76560000002, "isoelectric_point": 6.410324287414551 }, "id": "IGIGPGHMMC_708", "locus": "GCA000148625_00681" }, { "type": "cds", "sequence": "contig_1", "start": 385951, "stop": 386415, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTADEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "d58bf7ad8ae3df16d904eca596400295", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 385915, "stop": 386676, "observations": { "mutation": [ 385953, 386430, 386445, 386469, 386547 ], "stop": [ 385953, 386430, 386445, 386469, 386547 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.935, "score": 271.0, "evalue": 2.78e-90, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8932806324110671, "score": 432.0, "evalue": 5.35e-156, "description": "Internal stop codon at 385953, 386430, 386445, 386469, 386547. Nonsense mutation around 385953, 386430, 386445, 386469, 386547.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17768.86899999999, "isoelectric_point": 5.962433052062988 }, "id": "IGIGPGHMMC_709", "locus": "GCA000148625_00682" }, { "type": "cds", "sequence": "contig_1", "start": 386545, "stop": 386676, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "IGIGPGHMMC_710", "locus": "GCA000148625_00683" }, { "type": "cds", "sequence": "contig_1", "start": 387612, "stop": 388289, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCAAGTAGTTGAAGTAACTAAAGAAATACCAACAACTCAATTAGAAATTCTTGAAGCAACAAACCAAGAAATTAAAGAAGCAAATGAAGTTATTGAAGAAGAAAATCAAGTTAAAGCAATAATAATTAATGAAAATAGTCAAGTAATTAACGTTAATGAAGAAGACTTTATCAGTCAAGAACACTCAGATACTATTATAGTTGAAGAAATTAAAGAAGTGCCAGTTTTAGATAATGAAAAGAAAGATGAAACTATTGAAGAAGTTTTAGAAAAAATTAGCCAAGATCGTGAAAACACCAAAACTCAAGAAATTGAAAAATCTAAAGAAAATACAAAAGTTGTTGAAGCTATCAAAAAAGAAGAAACAGCTCAAGACACAATTACTTTGAATGCAGAAAAAACTCAAGAAGTTGGTTTTATTAAAGATGAAGCAACTATGGTTATTGAGCTTAATGTTGAAGAACAAAATGAACAAATTCAAAGTGTTTCACATTCGACTGAAAACACTATAAAACAGGGAGAAACAAAAAATATTGAAGCTGAAAATGCAATTGAAGAACAAGAAAAAACACTTCAACAATTAATCGAAGAAGGCGATAAAATTAAACAAAGTGATTATCTTGAAAGAGAAGAAGTTCCAGTTAAAGATTTTGAAGAATCAACAAAGGAATGA", "aa": "MTQVVEVTKEIPTTQLEILEATNQEIKEANEVIEEENQVKAIIINENSQVINVNEEDFISQEHSDTIIVEEIKEVPVLDNEKKDETIEEVLEKISQDRENTKTQEIEKSKENTKVVEAIKKEETAQDTITLNAEKTQEVGFIKDEATMVIELNVEEQNEQIQSVSHSTENTIKQGETKNIEAENAIEEQEKTLQQLIEEGDKIKQSDYLEREEVPVKDFEESTKE", "aa_hexdigest": "6e44bd5f5fe0466225f4468c7b17452c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25885.096700000024, "isoelectric_point": 4.2753381729125985 }, "id": "IGIGPGHMMC_711", "locus": "GCA000148625_00684" }, { "type": "cds", "sequence": "contig_1", "start": 388344, "stop": 388643, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCATTGAAACTGAACCAGTTGAAGTTGAGAATTCATTATTAAATCAAGAAATCAAAAGCACAATTCTTGAAGCAATTAAAGATAAACCTTATCTAAAATTAATTCCAAATGAAACACTTGGAACACTTAAAGTTGATTATGTTTTAAGAAGAAAACATGAACCAATAATGACATTTATGATCGATGATTACAAATATGTTAATAATCCTGAAGACTTTGTTATTTTTAAAGACTTGTGTAAATTTATTAGATCTAAAAAATATATTGTTTATAAACTAGATCGAATTTTATGA", "aa": "MQTIETEPVEVENSLLNQEIKSTILEAIKDKPYLKLIPNETLGTLKVDYVLRRKHEPIMTFMIDDYKYVNNPEDFVIFKDLCKFIRSKKYIVYKLDRIL", "aa_hexdigest": "4c678a3d073495c3420da6f6874b85dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11841.784199999986, "isoelectric_point": 6.582262229919434 }, "id": "IGIGPGHMMC_712", "locus": "GCA000148625_00685" }, { "type": "cds", "sequence": "contig_1", "start": 388722, "stop": 389054, "strand": "-", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "nt": "TTGTTGTGCTTCAATGCTAAACAACAAGATTCAATTACCACAAGCATTTTCAAAAATCATCCTGAATTAGAAAAAGCAATTAATAATGGAAACTTGTTATTAAGAAACATTGAAAATATTCAAGGCGATGAAGCTGATTTAGTTATAGCTTCAGTAGCTTATGATAAAGATACAGCAATTCACTCAACTTATGTTGGACGTCCTGGCGGTATGAATGCGTTAAATGTGGCAGTTAGTCGTGCTAAGGATAAAATGATTGTTATTAAATCACTTAAATCTAGTGACTTAACTATAATGACAGGTAATGAAGATGCTATTATGTTCAAACATTGA", "aa": "MLCFNAKQQDSITTSIFKNHPELEKAINNGNLLLRNIENIQGDEADLVIASVAYDKDTAIHSTYVGRPGGMNALNVAVSRAKDKMIVIKSLKSSDLTIMTGNEDAIMFKH", "aa_hexdigest": "8fbb0e89d4f835c318bccc7213e428da", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00313CCABD", "query_cov": 0.990909090909091, "subject_cov": 0.8384615384615385, "identity": 0.55, "score": 114.0, "evalue": 1.37e-30, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI00313CCABD" }, "pscc": { "uniref50_id": "UniRef50_UPI00313CCABD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_713", "locus": "GCA000148625_00686" }, { "type": "cds", "sequence": "contig_1", "start": 389511, "stop": 389861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAACACATTTAAAGAATTTCAAGGAAATTTAAATGATTCAGTTCAAGTTTTAAAAAACTTAAAGCAAGAAGACTTTGAATTTTTAAATGATTTAATAAACATTTTGCCTTCTAATAATACACAAGAAACTAGCGATGAAAATGAATTAAAGAAATTTATTGCTCAAGGTATTATTAAACGAATTAACAAATTTACTGATGAAGAAAAAGATCAATACTCAGAATTTGCAGCCACAATTAGATTAGGAACATTAGAACCTTACAAGTTTATTAAGCGTTTTCCTAATATGATTAAAAAGATTTTCCCTATTGTAATTATGACACCCGAAGCTGACTTAAGTGCCTGA", "aa": "MFNTFKEFQGNLNDSVQVLKNLKQEDFEFLNDLINILPSNNTQETSDENELKKFIAQGIIKRINKFTDEEKDQYSEFAATIRLGTLEPYKFIKRFPNMIKKIFPIVIMTPEADLSA", "aa_hexdigest": "45a3115ea5e4adcc554a497757c72b7c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13555.349199999979, "isoelectric_point": 5.02566967010498 }, "id": "IGIGPGHMMC_714", "locus": "GCA000148625_00687" }, { "type": "cds", "sequence": "contig_1", "start": 390042, "stop": 390368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAACCTTTTAGTTTATGGCAAAAATTCTTTAGTTTGTTCTCAAAAGAAAGCGGCTCTTGAAGTTATTAAAAATAGAATGGGCGCTTTAAAAATCTTTTGTCTTTTCATGCTTAATTCAAAAAACATGAACAAAAAGAATTTCTACTTGCCTCTTAGATTATATTTAGATTATTTAGAAAACTATGATGATAATCCTAATTTAAAACCATTAAGAATCATTAACCAAAAAGATATTCGTTTTGTTAACAATATAGCTAATTTCAGTAGTGATCCACGTTTTGTTAATATCACTCAAGCTTTAGCTAAAGTTCATAATAATTGA", "aa": "MTNLLVYGKNSLVCSQKKAALEVIKNRMGALKIFCLFMLNSKNMNKKNFYLPLRLYLDYLENYDDNPNLKPLRIINQKDIRFVNNIANFSSDPRFVNITQALAKVHNN", "aa_hexdigest": "3eff45d2e89c1ac5a9a7caa84b3440cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12584.632099999977, "isoelectric_point": 9.805786323547363 }, "id": "IGIGPGHMMC_715", "locus": "GCA000148625_00688" }, { "type": "cds", "sequence": "contig_1", "start": 390411, "stop": 390665, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGAACCAGGCGTAGTTTTAGGCTTATTCCAACCAAGTGGTGGCTATGCCAGAAATAGAATGCTTGAAATAATTAAGAATAAAGAATTGAACAAAATTTTAGATATAGAATTTAACAAAAACATCTATAAAAATAGGGTAAAACAAACAATTTACAACCCTAAAACATCTATTTTTAAAATTACACCTACCAACTATTCTCAAGATAAAGCTATAGCTTCAAGCTTAAATCAAAACACAATTATTTGA", "aa": "MEFEPGVVLGLFQPSGGYARNRMLEIIKNKELNKILDIEFNKNIYKNRVKQTIYNPKTSIFKITPTNYSQDKAIASSLNQNTII", "aa_hexdigest": "161ac7f1ea6832377a31b4414712c963", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9665.068599999991, "isoelectric_point": 9.646098136901855 }, "id": "IGIGPGHMMC_716", "locus": "GCA000148625_00689" }, { "type": "cds", "sequence": "contig_1", "start": 390783, "stop": 390989, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTAGGTTTCTTATTTGTTAAATTAGTTATTGACGATAAAACTATTTATGGTCCTTTATTTTTAAAAGAAGTTTATTTAGAAATTAGAAATGCTAGACCATATTTAGTTTCAAATGGTGACATTAAAATTAATGAAAAATTGCTATTCTTATTAAGTAATGCAGGTTTTGATTTAAATATCTCGGAAAACTATGGTGATTGA", "aa": "MHLGFLFVKLVIDDKTIYGPLFLKEVYLEIRNARPYLVSNGDIKINEKLLFLLSNAGFDLNISENYGD", "aa_hexdigest": "71509b96b76b2549cee3d36e92eeff12", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7821.9932, "isoelectric_point": 5.184250450134276 }, "id": "IGIGPGHMMC_717", "locus": "GCA000148625_00690" }, { "type": "cds", "sequence": "contig_1", "start": 391044, "stop": 391421, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACAATGAAGAAAAACAATATCAAAGTATTCTAGACAATTTACTAGATGTTAGTCCAAATGACAGTGCAGTATTTACTAGAGTAAATAACAAATTTGCTTTTGATTTTTATACTATTTTTGGACCTGAAAGTTTTAGCAAAATTTACAACAATAAAAACTTTGACATACCTATTCTTGAAGTTAATTTGATCAGAATTTGTAATTTACTTGAAGAAGCAAATACATATGAAGAAATTATTAAAATTCTTGAAGAAAACAATATTGAAGTAAGTGAAAAACGTAATAGTCAATTAAAGAAAAATCCTCTTGGAATTAAAAATAACATTATTAATGAATTAAAAGTTAATTTTCAAAAACAATCATTAAAATGA", "aa": "MSNNEEKQYQSILDNLLDVSPNDSAVFTRVNNKFAFDFYTIFGPESFSKIYNNKNFDIPILEVNLIRICNLLEEANTYEEIIKILEENNIEVSEKRNSQLKKNPLGIKNNIINELKVNFQKQSLK", "aa_hexdigest": "ce8210b51537f9379f31bdd9e1b7bf39", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14605.367199999982, "isoelectric_point": 5.0644338607788075 }, "id": "IGIGPGHMMC_718", "locus": "GCA000148625_00691" }, { "type": "cds", "sequence": "contig_1", "start": 391629, "stop": 391769, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGAAAGCGATGAAAAATTCATCGGTGGAGTAATACCATTTATCCAATTCTTTACACACATTGGTGTTGTGGTAGTTTCTATTGTTGGTATTATTATTGAAAGCGTTAAAGGTAAAGAAAAGAAAAATGTTTGA", "aa": "MTKESDEKFIGGVIPFIQFFTHIGVVVVSIVGIIIESVKGKEKKNV", "aa_hexdigest": "ece4e6e2520054c07d59d020cc718c29", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.974999999999, "isoelectric_point": 8.121743202209473 }, "id": "IGIGPGHMMC_719", "locus": "GCA000148625_00692" }, { "type": "cds", "sequence": "contig_1", "start": 391902, "stop": 392099, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGTAAGTGAAAAAAGTGGAGATGCAGGTAAGTTTTTAATAGCAAACAGACTTGTAACAAATGGAATTTTTGCAGACTTTTCAAACTTCACAAGTAATTTTTCAATAATTAAAAGCGTAGTTGAATCAGACACAATGGTTTATATAGCTTGTGGAATCTCAGCATTGTATTTTATCATTTATATTTCATGA", "aa": "MAKVSEKSGDAGKFLIANRLVTNGIFADFSNFTSNFSIIKSVVESDTMVYIACGISALYFIIYIS", "aa_hexdigest": "b777bb95341859c8d374dde6e0ccc2be", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.114299999999, "isoelectric_point": 5.997616386413574 }, "id": "IGIGPGHMMC_720", "locus": "GCA000148625_00693" }, { "type": "cds", "sequence": "contig_1", "start": 392100, "stop": 392204, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAAGGCAAAAAAATTAAATACGCTTCTGTCATTACTTTGTCAATTATTTTAGCAATATTTACTGTTTTATTAATTATTGTTGCTAAAGGAAGTTGA", "aa": "MSDKGKKIKYASVITLSIILAIFTVLLIIVAKGS", "aa_hexdigest": "fadfcd6737dfef9ae20a803efaf1bb28", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3635.4870999999994, "isoelectric_point": 10.000287437438963 }, "id": "IGIGPGHMMC_721", "locus": "GCA000148625_00694" }, { "type": "cds", "sequence": "contig_1", "start": 392920, "stop": 393090, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTTTAATTAAAAAAGGACATACTAACATTTCATATCGAGATGAAAATAAGTTTATTCAAGAAAAAATTTTAAATAAATTTAATCACAAATTAGAATACAGTTTATTAAAAGATTTTAGTTTTACACCAAAACTTATTAGCCAAAATGACAAAGAAATCACTTGA", "aa": "MKVLIKKGHTNISYRDENKFIQEKILNKFNHKLEYSLLKDFSFTPKLISQNDKEIT", "aa_hexdigest": "96cc984c8c4b9940453da2d23516638b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6727.7394, "isoelectric_point": 9.522963523864746 }, "id": "IGIGPGHMMC_722", "locus": "GCA000148625_00695" }, { "type": "cds", "sequence": "contig_1", "start": 393178, "stop": 394149, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor 4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "nt": "ATGGACAAAAGCAAAATTAGAAATTTTTCAATTATTGCTCACATAGATCATGGTAAAAGTACGCTTGCTGATAGAATTCTAGAAATTACTAATACAGTTAGTGACCATGATATGGATGATCAATTTTTGGACCAAATGGATTTAGAACGTGAAAGAGGAATCACAATTAAATTAAATGCTGTACAAATTAAGTATAAAGATTACGTATTCCATTTAATTGATACACCAGGTCACGTTGATTTTACTTATGAAGTTTCACGTTCATTAGCTGCTAGTGAAGGAGCATTATTATTAGTTGATGCTACTCAAGGAATTGAAGCTCAAACTTTAGCTAATGTTTATTTAGCTCTTGAAAATAATTTAACTATTATTCCTATTATTAATAAAATTGATTTACCTTCAGCTGATATTGAAGCCACAAAAAATGAAATAGAAGAAGTGATTGGTCTACCAACTGATAATGCAGTTTGCATTTCAGCTAAAACTGGTTTAAATTGTGAAGCAGTTTTAGCTGCAATTGAAAAATATATTCCAGCACCTAAAGATGCTGATGATTCTAAACCTCTTAAAGCTTTAATTTTTGACTCGTACTTCGATGAATATCGTGGTGTAGTTATGCTTATAAGAATTTTTGAAGGTGAATTAAAAGTTGGCGATGAATTTATGTTTATGTCGAATGGACAAAGATATTTTGTTAGTGAACTTGGAGTTAGAAGTCCAAAAGAAACTAAGAAAAAGAGACTTGAAGCAGGTGAAGTTGGATATGTAGCAGCTACTATTAGAGATGCTAGAGAAGTAAGCGTTGGAGATACTATAACTTTAGTTAATAGACCAGCTGAAAAAGCACTGCCAGGTTACAAAAAGAAAAAACCTGTGCTTTATACTGGGTTTTATCCAATTGATACTCGCGACTATATGGAACTTAAAGAAAGTTTAGAAAAAATAGCTCTTTCAGATAGCTCAATAACTTGA", "aa": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSIT", "aa_hexdigest": "3da9e17740625522f70716f9a6309cba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BB04", "query_cov": 0.9256965944272446, "subject_cov": 0.9373040752351097, "identity": 0.833, "score": 494.0, "evalue": 1.66e-174, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A448ZZP8" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "product": "Elongation factor 4" }, "genes": [], "id": "IGIGPGHMMC_723", "locus": "GCA000148625_00696" }, { "type": "cds", "sequence": "contig_1", "start": 394204, "stop": 394977, "strand": "+", "frame": 1, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "SO:0001217", "UniRef:UniRef50_Q8GCP5", "UniRef:UniRef90_Q8GCP5" ], "nt": "ATGTTACATATGGAGATATTGCAAGAGAGATTAAATCGTGAATATAAAATCGGTATTATAGCAACTAGTCCTTCAGTTGAATATGATGTTCATATGACAGATGGCAGCATTAAAAAAATATCAAACCCTGCACTTTTACCTGATCGAACTTTTATCGATCATATTGATGAACCTTACATTAAAGCTGAGATTTTGGTACCTAATGAATACATAGGAAATGTTATGGAACTATGTCAAAATAAACGTGGAATTTATATCAATATGGACTATATTGATGCTAATAGATCAAGGCTTATTTATGAAATGCCACTAGGCGAAATTATCTTTGATTTCTTTGATAGACTTAAGAGTCTAACTAAAGGTTATGCATCATTTGAATATGACCTAATAGGCTATAAAACAAGTGATTTGGTAAAAGTAGACATACTTTTAAATGGTGATAAAATTGATGCTTTTTCAATTATTACTCATAAAGATTCAGCTTATAGTAAAGCTCGTGATTTAACTCAAAAATTAAAAGAAGCTATACCAAGACAAAACTTTGAAGTACCTGTTCAAGCAACTATTGGAAGTAAAATTATTGCTAGAGAAACTATTAAAGCTTATCGTAAAGATGTTACTTCAAAACTTCATGCAGCTGATGTTTCAAGATATAAAAAACTTTTAGAAAAGCAAAAAGCGGGTAAGAAAAAAATGAAAATGCTTGGTACTGTTGAAGTTCCTCAAGAAGCATTTTTATCAATTCTTAAAACTAATATTGATAAGAAAAAATAG", "aa": "MLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK", "aa_hexdigest": "fc4c3be7f24ef9cedbc56b387e762b6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8741496598639455, "identity": 0.767, "score": 399.0, "evalue": 4.02e-138, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 393904, "stop": 394977, "observations": { "mutation": [ 394147 ], "stop": [ 394147 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8GCP5", "query_cov": 1.0, "subject_cov": 0.4290484140233723, "identity": 1.0, "score": 501.0, "evalue": 7.02e-174, "gene_start": 1, "gene_end": 257, "reference_start": 343, "reference_end": 599, "reference_sequence": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSITWEQETSKALGFGFRVGFLGMLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK" }, "paralog": false, "identity": 0.9971988795518207, "score": 695.0, "evalue": 9.16e-253, "description": "Internal stop codon at 394147. Nonsense mutation around 394147.", "psc": { "uniref90_id": "UniRef90_Q8GCP5", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q8GCP5", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8GCP5", "EC:3.6.5.n1", "UniRef:UniRef50_Q8GCP5", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "pscc": { "uniref50_id": "UniRef50_Q8GCP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8GCP5" ], "product": "Elongation factor 4" } }, "id": "IGIGPGHMMC_724", "locus": "GCA000148625_00697" }, { "type": "cds", "sequence": "contig_1", "start": 395332, "stop": 395562, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354584.1", "SO:0001217", "UniParc:UPI0001E330CC", "UniRef:UniRef100_A0AB32XBB6", "UniRef:UniRef50_A0AB32XBB6", "UniRef:UniRef90_A0AB32XBB6" ], "nt": "TTGAAAAGCAATGCTAAAGTTAAATCAAAAATTAAACAAGTAAAAATTAATAATTTCAAAATAGTTAAATTAACAAAATCTTTTGAAGAATTATTTTTTGGAGTTAATGATTCTTCAAAATTATCGGCCAATAACGTTATAACTTTAGATCAATATGTTGATAATTTAAACTTATTTCAGAGCAAAATTAATCTTAATTTAAATTTAATTAATAAAGAACTAAAAATTTAA", "aa": "MKSNAKVKSKIKQVKINNFKIVKLTKSFEELFFGVNDSSKLSANNVITLDQYVDNLNLFQSKINLNLNLINKELKI", "aa_hexdigest": "a839b59558589508e25b624123782c0d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330CC", "ncbi_nrp_id": "WP_013354584.1", "uniref100_id": "UniRef100_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330CC", "RefSeq:WP_013354584.1", "UniRef:UniRef100_A0AB32XBB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBB6", "uniref90_id": "UniRef90_A0AB32XBB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBB6", "uniref50_id": "UniRef50_A0AB32XBB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBB6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8730.137599999995, "isoelectric_point": 9.875218772888182 }, "id": "IGIGPGHMMC_725", "locus": "GCA000148625_00698" }, { "type": "cds", "sequence": "contig_1", "start": 396026, "stop": 396358, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354585.1", "SO:0001217", "UniParc:UPI0001A8C1FC", "UniRef:UniRef100_C4XG79", "UniRef:UniRef50_C4XG79", "UniRef:UniRef90_C4XG79" ], "nt": "ATGTTTTCAATAGTTGCTAATGATAAATTTTTGGGCCTAGCAGAAAGTGCCAAAAAATTTAAATATGTATTACAAGATTTAAAAAATAAAGGCGCTACTAACTACGAATGTGTTGAACGAAATATTTTAAAAACCGTATTTAATAGTAAAAAAACACAAATATTTTTATATCAAGAAAATAATAATTTGAGAGCAGAAAATATTGTTAATTTCAATCTTAAATATTTTAGCAATAGTGTTAAAAATGAATTAAATGATTCTTTACTTTCTAATGATGATTTAATAAAATTAAGCTTGAAAAAGCTTATGTTGAAATTACCAAAATATCTTTAA", "aa": "MFSIVANDKFLGLAESAKKFKYVLQDLKNKGATNYECVERNILKTVFNSKKTQIFLYQENNNLRAENIVNFNLKYFSNSVKNELNDSLLSNDDLIKLSLKKLMLKLPKYL", "aa_hexdigest": "8efe922e4c33b3d09deb2d5d138b68db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1FC", "ncbi_nrp_id": "WP_013354585.1", "uniref100_id": "UniRef100_C4XG79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1FC", "RefSeq:WP_013354585.1", "UniRef:UniRef100_C4XG79" ] }, "ips": { "uniref100_id": "UniRef100_C4XG79", "uniref90_id": "UniRef90_C4XG79" }, "psc": { "uniref90_id": "UniRef90_C4XG79", "uniref50_id": "UniRef50_C4XG79" }, "pscc": { "uniref50_id": "UniRef50_C4XG79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG79" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12802.756799999983, "isoelectric_point": 9.520449256896974 }, "id": "IGIGPGHMMC_726", "locus": "GCA000148625_00699" }, { "type": "cds", "sequence": "contig_1", "start": 396743, "stop": 398728, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80", "UniRef:UniRef90_C4XG80" ], "nt": "ATGTCTGATGATGATTTTCTAAAAAATTTAGAAATTAAAGGACAAAATGTAAGTGAAATTTATGCTAGTGATGCAAAAATTACAAATGTTGAATACAAAAAAGATTCTAATTATGAAATTAATGTTTCTCTAGAAGGATCAAATGTTCTAAAATCAACTGCATACTACAAAATTGAAATTACAAATAAAAATACAAAAGTTAAAAAGTCAAAAATTGTTCCAATAAGTGGTTTCAAGAAGTTTGATGAAAAAAGTACTATTTTAAAAGAAAAAGAGCTATTTGAAGATAAATCTAAATTAAAAGAATATTTAGATAATATGCCTAAAAATGATTCTGAAAATCTTAAAAAATATATTTCTAAAAATAAACATTTTATTTACAACGAAATACCAAGTTCATTAAAAGCTTCATTTAAAGGTTATTCTTTCATTCAATCTAATGGTCAAGATGCTGTTAGAGTTGTTTTCGTTTTAAATGGTCATATTACAAATGATAATCTCGAAAATAATATAGTTAAAAAAGCAAATAAAGAATATAAAAATAATGAAAAAACTTTTGAATTTTACTTAAGTAATTTAGTAAAACAAGGAACATCAAGTTCAGAAATTCAAGCAAGAAATAACTTAAGAGATTTAAAAGAAAAATACGAAACTCTTAAAAAAGAAGCTGAAGTAGCTAAATCGAAAGTTACAGGTGCTATTGTTGCTGAATATGAAAATGTTTTAGTTTCAATTACTAAAAATATTAATGAAATCGACAAAATTTTAAATGATGCAACAATTAAAGCAGCAGATCATGTTGATACATTAAAAGTTAATTATGAAAAAGACTTATTAGATAAAAATACTGTAGATTTAAAAGCAACAATTAATGATGCTGAAGTTACAAGAGATAACTATAAAGCTAAAGATGAAACAAATTATAACAATCCAATAATTGAATTTAACAAAGCTATTGATCAAGCAAAAATTAAACTTGCTTCAACAAATATTAAAGAAATTAATGATGAAATTTCTAACTTAAAAACTAAAAAAGAAGAGTTTATTAATGCTTTAAAAACTATCTTAACTGACACACAAAGTGATTCAAAAACTAAATTGTTAGAATCAAGCGATTACAAATATTTGATCGATGATATTAAAGCTCGTAAAGCTACTTTTGATGCTACACTTAATGAAGATAATATTCTAAGTGAAATTGAAAACTTAGAAGTTTTAGTTCAAAATACTCAAACAATTGATGCTATTACACCAAAAGCAATAATTGAAGCTAATAATAAACTTCTAGCTTCCTTACTAGCCTATAAAACAGCCTTAGAAGCAAAATTACACAAAATAAAACAAGATGTAATAGACTCAACTTATGACAAAGATTCATTGTCACAATTTATCGAATATTTAATTAAAAAAATTAAAACAACAAATGAAAGCAAAGCTTTAGTTAATAAACAAATAGATTTATTTAAAGCTTATTTAACTACACCAGGTTTTGTTCAAAAATATAATTCTTTAGAAAAAGATTTTGATAAATATGAAGCAATTTTTAATGAAAATATAAATAAAGCTAATGAACTTATCGCAACTCTAGATGCAGGATTAGATCAATATTCAAATTCAAAAGATAAATATAGTGAAATCAAAACTAAAGTTGACTCACTTTTAGCAGAACAAGCAACTCGTTTAAGCTCAATTGGAACTCAAATTGTTAATAGTGCACTTATTGATTTTGATAATTATGTTCAAAAGCAAAAAGCTTTAATCAATACTTCACGTAATAACTACAAAACAATTGTGACAAATGTTTACAATTTATTCAAAGATTCAGAAATCTACAAACAATATATAGATGATAATAAAGCGAAACTTACAAACTCAACTATTGATGTTAAAACTGCTTTTGAATTATTAAAAACTTTATATACAAAAGATAATGAAATGAATCAAAAAGTTGCAGCTCACTCAAACTTAGCAACTAATAGCTACTGA", "aa": "MSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTGAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSY", "aa_hexdigest": "165cb101c63a783fac8c2633e6600e0c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 396623, "stop": 399031, "observations": { "mutation": [ 396665, 398726 ], "stop": [ 396665, 398726 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG80", "query_cov": 1.0, "subject_cov": 0.7099892588614393, "identity": 1.0, "score": 1223.0, "evalue": 0.0, "gene_start": 1, "gene_end": 661, "reference_start": 41, "reference_end": 701, "reference_sequence": "MSTKTISKATKGLGWAIATATITGSILLTIIAIKTQNTKKMSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTGAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSYWNSLLDGDLKNNFTIPMQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKNWQIYSNSINGPEHQDVFPKTIKLINESWKKLKMLLTIVQLLLLKN" }, "paralog": false, "identity": 0.9975062344139651, "score": 1540.0, "evalue": 0.0, "description": "Internal stop codon at 396665, 398726. Nonsense mutation around 396665, 398726.", "psc": { "uniref90_id": "UniRef90_C4XG80", "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG80", "UniRef:UniRef50_C4XG80" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 75369.16350000027, "isoelectric_point": 6.638475990295411 }, "id": "IGIGPGHMMC_727", "locus": "GCA000148625_00700" }, { "type": "cds", "sequence": "contig_1", "start": 398774, "stop": 399283, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTTAATGCTGATTATGAAAATTACTTTAAATTAGATGACCATTACAAAATGGCTTTAGATATTATTATGGACAATTTAATATCTCAAATTCGTTCAGAACATATTAAAGGTATTGAAGCGAAACAAGAAGAAATTTATCGTATTTTCTATGAAAAAATGCGTGCTATTTATAATGCACAATTAACTAATGGAGTTAAATTTGTAAATGATAATATTCTAGATGCTAAAAATAGAAGAATTCAAGCATTAAAAGAATATGAAAAGGCTTTAAAAGAAGCAGAAAATTTAATCAAACATTATACAGATGAACACTATAACAATACTGATGATTTCTTCAAAACACTTGAAGAAGGCATTAAAGCTGTTAAAACTTTAAATAAAGATCGAGCAATGCTAAGTCTTGTAAAAAATACAACTATAGAATTAAAAGAAGTTATCAAAAATTCACAAGATCAATATAGAACATTCCTATTGTCTAAACTTGATGAAGCTAGAAAAAATTGA", "aa": "MQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKN", "aa_hexdigest": "42864cbd500023e603dc397f908f3820", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20091.6405, "isoelectric_point": 6.448179054260252 }, "id": "IGIGPGHMMC_728", "locus": "GCA000148625_00701" }, { "type": "cds", "sequence": "contig_1", "start": 399362, "stop": 399484, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAATTAAAAATGCTATTGACAATAGTACAACTATTACTATTAAAGAATTAGAAGATTTAGTAAAAGAAAACAATGATGTATATGGAAAATATACAAGCACTAAAATTCCAGAATGA", "aa": "MEKIKNAIDNSTTITIKELEDLVKENNDVYGKYTSTKIPE", "aa_hexdigest": "e9f6967401b1ff1b986153fc06b609bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4587.117800000001, "isoelectric_point": 4.924950981140136 }, "id": "IGIGPGHMMC_729", "locus": "GCA000148625_00702" }, { "type": "cds", "sequence": "contig_1", "start": 399980, "stop": 400312, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAACAATAGTTTAGCAGCTGCTAAAAATAAATTACAATCATCAATTGAAAAGGTTGAAAATCATTTAAATTCAAGAAATTCTTTAGACAATTTAGATAATTCATATTACTCAAATTTAATGCAAAATGAGAAAATTGATTACAATGAAAATAGTTCAACTGCTGCTTCCAAAAAAGATTTAAAATCTATATTAGATTCAATGAAAAATATTGTTAAAGGCAATGATGAAAATGCAATTAATCACAATGTTCAATTTGGTCAAAAATTAATTGATGCTATTGAAGAAAAAAGAACAGAAGACATAGTTGCAAACAAAATACATTAA", "aa": "MNKNNSLAAAKNKLQSSIEKVENHLNSRNSLDNLDNSYYSNLMQNEKIDYNENSSTAASKKDLKSILDSMKNIVKGNDENAINHNVQFGQKLIDAIEEKRTEDIVANKIH", "aa_hexdigest": "d471a85847551cd7e344dac1f8b2d03a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12435.625299999989, "isoelectric_point": 6.2816976547241214 }, "id": "IGIGPGHMMC_730", "locus": "GCA000148625_00703" }, { "type": "cds", "sequence": "contig_1", "start": 400386, "stop": 400562, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00059F7513", "UniRef:UniRef90_UPI00059F7513" ], "nt": "ATGAGAGCTTCACGATCAGTTTTAATTTTAGTTAAATTACCAATTAATTTTTCTAATTCAACTTTAGTATTAACAATTGATTTTTGGCTTTCTTTAATTAAGTTAGCTCGTGCTAATTTTGTAATTCTATCAGCATCTTCATCAGCAGTTGAATTAGCTTCAAGATTTGTAATTTGA", "aa": "MRASRSVLILVKLPINFSNSTLVLTIDFWLSLIKLARANFVILSASSSAVELASRFVI", "aa_hexdigest": "05ee9f43f584cbb2ba5253366fecf2ef", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00059F7513", "query_cov": 1.0, "subject_cov": 0.9508196721311475, "identity": 1.0, "score": 100.0, "evalue": 5.11e-27, "valid": true, "uniref50_id": "UniRef50_UPI00059F7513" }, "pscc": { "uniref50_id": "UniRef50_UPI00059F7513", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00059F7513" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6393.583999999999, "isoelectric_point": 11.553846549987792 }, "id": "IGIGPGHMMC_731", "locus": "GCA000148625_00704" }, { "type": "cds", "sequence": "contig_1", "start": 400675, "stop": 401118, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACCTAAACTTGAAGCAGCAAATAATAAAAATGAATTATTAAAAGAAGTTTCTAAAGATCAAAATAATAGTAATATAACAGCTAAAATTAATGATTCTAAAGTTGCAATTCAAGATTTAGAAAATGTTGTAAACAACTATAAAACAGCTCTTAACAACAAAATTAAAGAAAATATTTCAGCAATAAAAGAATTAGTACAATACATTAGAAACAATCCAAACAATTTAACTCTTCCAGCTAATTTTGTAACCAATTTAAAAGCTGTAGAAGACAATCAAGACTCAACTGATATTGCTATTTTAAATCAAACAAAAACTCAAAGTGAAGCATTAATTAAAGAGTTAACCGATTTAATTAATACAGCAGTAGCAACTGTTAATAATAAAGCTCAAGATACAAATCAAAAAGTTGAAGAAGAAAAAGCAAAAAATGAAACATGA", "aa": "MKPKLEAANNKNELLKEVSKDQNNSNITAKINDSKVAIQDLENVVNNYKTALNNKIKENISAIKELVQYIRNNPNNLTLPANFVTNLKAVEDNQDSTDIAILNQTKTQSEALIKELTDLINTAVATVNNKAQDTNQKVEEEKAKNET", "aa_hexdigest": "d24074c9a48879155bc33bc9aa2ecef5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16406.140199999987, "isoelectric_point": 5.307647895812988 }, "id": "IGIGPGHMMC_732", "locus": "GCA000148625_00705" }, { "type": "cds", "sequence": "contig_1", "start": 401119, "stop": 401481, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGCTGCTCAAGTTCGAGAAATTCAACAACTTTTTGATAATTCTCAAGCCTTATTTAATGAAGTTAAAAACGCTGCTAATAATAAAGATCAAATCAAAGATGTAGGTGTTGATAACTTAACAAATAATATAACTAAAGCAGAAGCAAAATTAAAAGAAATTCAAAACAAAAAAGCAGAATTTAAACAAGAAAATACTAATTTAAGAAATCAAATTAGTGAATTAATGAATGATTTAAAAGTACAAGCTGATACACTTCCAAATGAATTTAATGAACACATTTTAGCAACTCATAGCTCAATAAAAACCATTCAAGAATATTATGAAAATACATATAGCAAAATTAAAAATCAAGAATGA", "aa": "MTAAQVREIQQLFDNSQALFNEVKNAANNKDQIKDVGVDNLTNNITKAEAKLKEIQNKKAEFKQENTNLRNQISELMNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQE", "aa_hexdigest": "ab596ef062621d7a619a955054727c3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13842.224799999987, "isoelectric_point": 5.450825309753417 }, "id": "IGIGPGHMMC_733", "locus": "GCA000148625_00706" }, { "type": "cds", "sequence": "contig_1", "start": 401632, "stop": 402903, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6", "UniRef:UniRef90_UPI00217F41A6" ], "nt": "TTGACTACCGCTAATGATGAATTAACTGGTATTAATAAAGTTGATAACTATTCAGATTCGATTACTAAATTAAATAATCAAAAGAAAGCAGAATATCAAGAATTAATTGATCAAAATAACAATGATCTTAAAACTCTTAAAAATGCTTTAATTGAAATTAAAAATAGTAATGACTTAATTGAAATATTAATTAATGAAGTTGTCAATAAAAAATGTAACTTAATTAATGCAACTATTAAAGAATATGAAGATCAACTAGAAGCAATTAATAATTCTATTTACAGCGATTTATATGAAGACATTACAACAAGTTTACATTTAGACTTTTTAAACTCTTTAGCTATTTTAAAAAATAAATATCCAAATAATTTAAATAAAGTAATCAAACTTTCAGAAGTAAAAAGAGATTATGAAGAAATTGAAGGTAAACTTAATGATTTAAAAAATAGATTAGCCGCTACACAAAATGAAAGAAATGACATTCTTGCAGAATTAAGATTAAACTTTGACAAAATTAATTCATATGCTAATTATTCAGATGCTGATAGTAAATTGCCTTTAACTAAAGCTTTAATAAATGATGCAAATAATAATTTAGAAAATCACTTTAGTTCAATGACACTAAAATACTTAAAAGCTGAAAAATCTGCTAGTGGTCAATATACAAAAGTAATAAATGAAATAAATCAATATGAAGCAGATCTTAATGTAGCTTTACAGGAAATTATTAATGAAGTAAAAGATGCTAAATATCAAAAAATAGATGACATTATTTCAAATAATTCAAGTGTCTTTTTAGCTGACAAAGATGATTATAAAAAAACAATTGCACCAGTTAAACAAAAAGCTAATAATGGTGTTAATGACTTAAAAACTTTAATTACAACAATTAAAGATGCAAATGAAAAACTTGTAGCTATTAAAGCTCTTGGCAATAGAATTCTTACAAAAATTAATACTGAATTCAACAATAGAATTAACCGACTTAAAACTATTGCAAATGAAACTGATGGATTCTTAAGTCCTAAAGTTGCTGACGGTAAATATGAAGGTTCTATTTTGCATGGAATTAAACAATCAAATGATGAAATTATTAAAATAATTAAAACAAAAATCGATACAATAATGAACAATAATAGTAACCTTGCATACTTAGTAAAAATCGATAATTCTAGTGATAAAACTATAGAAAAATTAGATCAATTAATTGCAAGAGCAACCCAATTTAAAGATAACGCAATCACAAAAACTAATGAATATGAAAGAGCATGA", "aa": "MTTANDELTGINKVDNYSDSITKLNNQKKAEYQELIDQNNNDLKTLKNALIEIKNSNDLIEILINEVVNKKCNLINATIKEYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLAILKNKYPNNLNKVIKLSEVKRDYEEIEGKLNDLKNRLAATQNERNDILAELRLNFDKINSYANYSDADSKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERA", "aa_hexdigest": "f639a5a7d28221397cf1996faf0ae93d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 401332, "stop": 403206, "observations": { "mutation": [ 401479, 402901 ], "stop": [ 401479, 402901 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217F41A6", "query_cov": 1.0, "subject_cov": 0.4890173410404624, "identity": 0.95, "score": 751.0, "evalue": 1.02e-265, "gene_start": 1, "gene_end": 423, "reference_start": 173, "reference_end": 595, "reference_sequence": "MNDSCSSSRNSTTFDNSQALFNEVKNAANNKDQIKDVGVDNLTNNITKAEAKLKEIQNKKAEFKQENTNLRNQISELMNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQEWENKNINDLQAIKDILDGYKQSLDNYKNEAAAAYRNELKEQIQKSNNKVQKLTTANDELTGINKVKNYSDLITKLNNQKKAEYQELIDQNNNVLKTLKDALIEIKNSNNLVETLINEVVNEKCNSINAIIKKYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLATLKNKYPDNLNKVIKLSEVKRDYEEIKSKLDDLNNRLAATQNERNNILAELRLNFDKINSYANYLDADTKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERAWEEAKNENLQKLDEANGIIQFLNDKKTTSTITSEEQTALTALNTAKQNYDRIIENNIDHKSKTKIEDATNHLNDALNTARQELKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIDEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIELDEITNQELKDKVTAKFNEFKADIVNFCSQNITNYDADVATLTTKHQTIQNKWNVDSEMIKIGKYLKYVVVKQL" }, "paralog": false, "identity": 0.9583333333333334, "score": 1157.0, "evalue": 0.0, "description": "Internal stop codon at 401479, 402901. Nonsense mutation around 401479, 402901.", "psc": { "uniref90_id": "UniRef90_UPI00217F41A6", "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217F41A6", "UniRef:UniRef50_UPI00217F41A6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 47995.530699999996, "isoelectric_point": 5.4840192794799805 }, "id": "IGIGPGHMMC_734", "locus": "GCA000148625_00707" }, { "type": "cds", "sequence": "contig_1", "start": 403147, "stop": 403647, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATTCACAAAAAACTACTGCAATTATTAATTTAAAAAACCTTAAATCAGAAATTAATGCAGACTTAGCAAAACTAAAAGGCTATTCATCATTATCAAATGATCTTACTAAATCAATTGGTGATAGCTTAGTTCAATATAAAGCTTCATTACAAAAAGTAATTGATGAAATTGATGATTTAGTTCAAAAAATCAATACCATAGGTTTTGTTCAAAGTGAATATGATACTGTTTATAACAAATATAAAGATGTACCAAATAAAGACGAAAACATCATTAAATTTATTGAAAATAAAGTATTTGTTGAAACTAAATTCTTAAACAAATATGACATCAAATTAGATGAAATAACTAATCAAGAATTAAAAGATAAAGTTACAACTAAATTTAATGAATTCAAGGCAGATATAGTTAACTTCTGTTCACAAAATATTACTAATTATGATGCTGATGTTGCAACACTTACAACAAAACATCAAGCAATTCAAAATAAATGA", "aa": "MKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIDEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIKLDEITNQELKDKVTTKFNEFKADIVNFCSQNITNYDADVATLTTKHQAIQNK", "aa_hexdigest": "06df3394339a258b2cd19331fb00a180", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19013.329100000003, "isoelectric_point": 6.598859214782716 }, "id": "IGIGPGHMMC_735", "locus": "GCA000148625_00708" }, { "type": "cds", "sequence": "contig_1", "start": 403663, "stop": 403914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAATTGGTAAATACCTTAAATATGTTGTTGAAGCAACAAACTTTGAAAAAGCTATTCAAGCAATTGAAAATTCATATAACAATGAACCTAGCGGATACAAAGATGTTGAAGCTTACAATAACTTCAAAAAAGAAGAACAAAACTTAGCTTCTGGAAAAACATCTATTGAAACAATACTTAACAATTTCAAAAACAAAAAACAAAGTGAATTAATTAGTGGAAATGTTCCTGAAGATGCTCAATGA", "aa": "MIKIGKYLKYVVEATNFEKAIQAIENSYNNEPSGYKDVEAYNNFKKEEQNLASGKTSIETILNNFKNKKQSELISGNVPEDAQ", "aa_hexdigest": "01ee9d39c6e4db39b23adda7938e5d25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9421.390299999994, "isoelectric_point": 5.436388206481933 }, "id": "IGIGPGHMMC_736", "locus": "GCA000148625_00709" }, { "type": "cds", "sequence": "contig_1", "start": 403948, "stop": 404307, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATAATAATCAAATAATAACAAATAAATTGCGTGAATTTGATGAACAAAATATTGAAAAATTAAGAAACTATGTTGAAGAACTTAGACAATTAGTAAATATTTATGAACATGCTTCAATAAAATTTGCAAGCACAAATTTAAATGACATTAATAAAGAAATTAGTGAAGTTGATGCTTTAATTGCAAATAGCAAAAAAGCCTATAAAACAGAAGATAAAGTAAAAAATAGCGCTGCAAACTTAAGTCAAAAAACACTTAATACTTACAATGAATTTAGAACTCAAACACTGAATAAATATAAACAATTAATGAGTCTAAATGTTACTCCTGTTGTAGCAAATGTTAGACCTTGA", "aa": "MSNNNQIITNKLREFDEQNIEKLRNYVEELRQLVNIYEHASIKFASTNLNDINKEISEVDALIANSKKAYKTEDKVKNSAANLSQKTLNTYNEFRTQTLNKYKQLMSLNVTPVVANVRP", "aa_hexdigest": "fc80d43e29d81d59d8ae6aedc9c0c765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13779.387299999977, "isoelectric_point": 9.125387001037598 }, "id": "IGIGPGHMMC_737", "locus": "GCA000148625_00710" }, { "type": "cds", "sequence": "contig_1", "start": 404686, "stop": 404874, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAATCGTCAATATAAAGAATTAAACGAAAAATACTTCTTTGAAAGATTAAATTCATCAAATAATGCTAAAATGACTAGTTTATTAGTAGATAACTACCACAAATACTTTGTTGATTTAAATGGTAAATTACTAGGTCAAGTATCATTCAATAATGAAGATGAAAAAATTAATAAATATAGATGA", "aa": "MHNRQYKELNEKYFFERLNSSNNAKMTSLLVDNYHKYFVDLNGKLLGQVSFNNEDEKINKYR", "aa_hexdigest": "4d1107b9a47eb967b608671f251a60ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7550.3743, "isoelectric_point": 8.963442420959474 }, "id": "IGIGPGHMMC_738", "locus": "GCA000148625_00711" }, { "type": "cds", "sequence": "contig_1", "start": 405052, "stop": 405225, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGACATGGCTTTAGATGAAGAAATGCTTGCTATTAATGATTCAAATCAAAAATTTGATGATGGTTATAGAACAGTTATGTTAGTTGGTGGATTTGCTCAATATGTTAAAGATCATCTTAATGACAAAAATTCAATTTATGCAGCTAATAGTGGTTTACAATTATGA", "aa": "MGYDMALDEEMLAINDSNQKFDDGYRTVMLVGGFAQYVKDHLNDKNSIYAANSGLQL", "aa_hexdigest": "058cc38f27be0fd0ac50babc20ee7b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6392.057600000002, "isoelectric_point": 4.265277671813965 }, "id": "IGIGPGHMMC_739", "locus": "GCA000148625_00712" }, { "type": "cds", "sequence": "contig_1", "start": 405865, "stop": 406287, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACATTCAAACATGTATTAGATTCTAGATATTCTCTTTTAGCAAATACTCCATATACTTATTGTTTAAATATTGAAAGTTTTGTTGCTTATAAAAATCACATAATTGATGATAATGCAAAAAAACAATATAACCAAATAGTTTCTAATATTACAAATAGAATGAATTCAGCTTCAAGCAAAGAACAAGAAGCTAAAAAAATATTAGAAGAAATAAACAATAAATTACAAACGTTAAAAAATAATCCTAATACGCACTATTTTGGAAATAAAAATGTAGTAGGTCGTTCAATTGATCAAAAGCCACATATTACATTTAATATTGCTATTTATGATGAAAATCAAAAACATGATTACTACATAACAAGTACAACTAATTATCGTTTCTTCTTAAAAGATGTTAGCAAAGATATTTATTAG", "aa": "MNTFKHVLDSRYSLLANTPYTYCLNIESFVAYKNHIIDDNAKKQYNQIVSNITNRMNSASSKEQEAKKILEEINNKLQTLKNNPNTHYFGNKNVVGRSIDQKPHITFNIAIYDENQKHDYYITSTTNYRFFLKDVSKDIY", "aa_hexdigest": "2d1dd985d0c8bdff229b3ccec7af2d6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16483.280699999992, "isoelectric_point": 9.042738533020017 }, "id": "IGIGPGHMMC_740", "locus": "GCA000148625_00713" }, { "type": "cds", "sequence": "contig_1", "start": 406336, "stop": 406497, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8", "UniRef:UniRef50_A0AB32XBM8", "UniRef:UniRef90_A0AB32XBM8" ], "nt": "ATGCAAGAAATCTTAATTCCTCTCCACTCTCCCCTTTATTCAATTAAAGGAGAGACCTTCTTTGAAACTAAAGAAGCAAAGCTTTTAAAATTTGAAGAAAAATTATTCTTCATGGGACATCACACAAAATTTTTTACAGTCCCGGTTCACATACCATTTTAG", "aa": "MQEILIPLHSPLYSIKGETFFETKEAKLLKFEEKLFFMGHHTKFFTVPVHIPF", "aa_hexdigest": "80a69bfdd031766b0fe6329d2d4678bc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001F5A628", "uniref100_id": "UniRef100_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBM8", "uniref90_id": "UniRef90_A0AB32XBM8" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBM8", "uniref50_id": "UniRef50_A0AB32XBM8" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBM8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6323.4245999999985, "isoelectric_point": 6.901811027526856 }, "id": "IGIGPGHMMC_741", "locus": "GCA000148625_00714" }, { "type": "cds", "sequence": "contig_1", "start": 407318, "stop": 407524, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAATTACTGAAAAAATGCTTTTAAATATCAAAAAAATATCAGATTCTAAAATAATGCTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGAAAGATGA", "aa": "MAKLIEYEKTITEKMLLNIKKISDSKIMLKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEER", "aa_hexdigest": "f64bc6f4bc27e9b20bf6785b8ea22649", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8181.370100000003, "isoelectric_point": 8.802851676940918 }, "id": "IGIGPGHMMC_742", "locus": "GCA000148625_00715" }, { "type": "cds", "sequence": "contig_1", "start": 407969, "stop": 408190, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAATTAAAAATAATCAAATTTCTAAATTAGATAGTCAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAACATTGATTTAATTAAAAATTATTTAAATGAAACAGATTATTTATTAGCTGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MIAIKNNQISKLDSQGLKALQQLDGGVDFTNIDLIKNYLNETDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "16de9c6bd8d0e41364b20c1cc8b94209", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8529.637399999998, "isoelectric_point": 4.791663551330567 }, "id": "IGIGPGHMMC_743", "locus": "GCA000148625_00716" }, { "type": "cds", "sequence": "contig_1", "start": 408302, "stop": 409084, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAACATTCTTTGAAATTATTTCAAATCTTAAAGAATATGAAACTAATTATTTAAAAATATTTGAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTCAATAACTTTGCTTCGAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCGAATCCAAAAATTGCTGATTTCTTATCAAAAATTTCTGATAATTTAAAAACATTAGACCAATTCGAAGATGTTAAAAAAATAATTGAAGATATTAATAATAACAAACCTAATTTATCAACTAAAGAAATATCTTCAATTGAAAATAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTATTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAACGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTATTGTTCAAGCTGATAAAGACAATAAAGTTAACTTTGTTAAAAAATGA", "aa": "MNEFNLALEANKTTLNGFNLFETSYKSIIDIQTKLDPLLKANLDSDIDNKINNISTFFEIISNLKEYETNYLKIFEIIKNATDLNLQKVNNFASKPKEISKLISNANPKIADFLSKISDNLKTLDQFEDVKKIIEDINNNKPNLSTKEISSIENSEIQNDKEKFKGLFEKINLINNFISSINEKDKLYSNFLENEAQFCLESYNEMDAKLAAIANVDSKVYEYKNKIFDAAIEAYKNVNSTENFSIVQADKDNKVNFVKK", "aa_hexdigest": "c96335deb1a366319cd915dc1d40e339", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29851.33310000003, "isoelectric_point": 4.9920778274536115 }, "id": "IGIGPGHMMC_744", "locus": "GCA000148625_00717" }, { "type": "cds", "sequence": "contig_1", "start": 409199, "stop": 409831, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGCGGTGTTTTAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTTAAAACAATTGAAAATACAAAATTATTTAATAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTCGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCAAAACAAATTATTGATCAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNVDSIETTVKLAKLNNSKQIIDQFVQKSKIELSSLK", "aa_hexdigest": "fb7e6d4e1dd83e29bd5a97f7ba3a45f7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 409001, "stop": 409831, "observations": { "mutation": [ 409201 ], "stop": [ 409201 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.976, "score": 381.0, "evalue": 2.31e-132, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9637681159420289, "score": 508.0, "evalue": 1.44e-184, "description": "Internal stop codon at 409201. Nonsense mutation around 409201.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23671.59690000002, "isoelectric_point": 5.213408851623535 }, "id": "IGIGPGHMMC_745", "locus": "GCA000148625_00718" }, { "type": "cds", "sequence": "contig_1", "start": 409886, "stop": 410350, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATATTGGTCACCACTTTGGTAACAAAAGTTATATAAGAGCTGTTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAGGAATATCAAAACACTTTAAGTAAAATTGAAGAAGAAGCACTTTCAAGCAACATAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCCGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHIGHHFGNKSYIRAVKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKEYQNTLSKIEEEALSSNIKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "667b65428f8842532550fc40bf57fbba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 409850, "stop": 410611, "observations": { "mutation": [ 409888, 410365, 410380, 410404, 410482 ], "stop": [ 409888, 410365, 410380, 410404, 410482 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.9420000000000001, "score": 274.0, "evalue": 2.4e-91, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9090909090909091, "score": 437.0, "evalue": 8e-158, "description": "Internal stop codon at 409888, 410365, 410380, 410404, 410482. Nonsense mutation around 409888, 410365, 410380, 410404, 410482.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17763.89219999999, "isoelectric_point": 6.07150707244873 }, "id": "IGIGPGHMMC_746", "locus": "GCA000148625_00719" }, { "type": "cds", "sequence": "contig_1", "start": 410480, "stop": 410611, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGAATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "IGIGPGHMMC_747", "locus": "GCA000148625_00720" }, { "type": "cds", "sequence": "contig_1", "start": 410987, "stop": 411208, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACTTCATAAAAAACTTAAAGATAAAATTCATAAATTATTTGCGGGATATGAAGAGTTTATATTATTAAAAGTAAATAATCTTTGTAGATCATTTTTTAATTGTTTTACTGAATTATTGTCCATAATTTTTCAATCAAAAGATAATAATGAATATAAACAAATTATTAGAATAAGCAAAATAAAAGTTACTTTAACTCATCTTTTGAGTTTTTTATAA", "aa": "MVLHKKLKDKIHKLFAGYEEFILLKVNNLCRSFFNCFTELLSIIFQSKDNNEYKQIIRISKIKVTLTHLLSFL", "aa_hexdigest": "a96987788128ee145e4ed8e57011b5a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8718.346999999992, "isoelectric_point": 9.596844291687013 }, "id": "IGIGPGHMMC_748", "locus": "GCA000148625_00721" }, { "type": "cds", "sequence": "contig_1", "start": 411957, "stop": 412475, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGCGAAAGTTATTCTATTTTCAAAGAATTACTAATTACTATCAAAATTACTATTGCCAGCATTTTCTTAGCTTTTATCTTTTCAATTTTTGCTTCTTTTTTTGCAAACGATAAAACAAATAATAAATTTGTGAAAATAATAAGTAAAATGCTTTTAGCATTCTTTAGAACTATACCAATAACCTTATTTTTCTATTTATCAATTAATCTTTATTATTCAAGAGAAGTCATTTTAATTTTAGCTTTATCTTTTACTACAATGCGTTCAATGAGTAAATTTTATACTGAATCAATTAATAATATTGATCAAAAATTAATTGACAATTATTTATTATTAAATAAAAATAAAATAACTCTATATTTTGAATTTATTTTACCTAGAGTAATTAAAGATTTTTATAGTTATGCAGCTTTTAGATTCGAATTAATCTTTAGAAACATTATTAATTATGGAACTTTTGCAGCTGTTGGTTTAGGTGCTAGATTAAATTATTACCAAAACAAAAATGAATGA", "aa": "MKSESYSIFKELLITIKITIASIFLAFIFSIFASFFANDKTNNKFVKIISKMLLAFFRTIPITLFFYLSINLYYSREVILILALSFTTMRSMSKFYTESINNIDQKLIDNYLLLNKNKITLYFEFILPRVIKDFYSYAAFRFELIFRNIINYGTFAAVGLGARLNYYQNKNE", "aa_hexdigest": "fb1404c167c5413ac0ca88a26f5967f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20321.73049999999, "isoelectric_point": 9.670209312438963 }, "id": "IGIGPGHMMC_749", "locus": "GCA000148625_00722" }, { "type": "cds", "sequence": "contig_1", "start": 412613, "stop": 412882, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACTAGCCTCAATCTTTTTTGAAAATAAAAATATTATTTTGGCTGATGAACCAACAAGTAATTTAGATTTTATTAATACTAAAAAAATATTAAAATATTTGCAAAAATATGTTCATGATAATAATGCATTATTATTATGTAACATTCATGATGTTGCTAATTCAATAGAATATATGGATTATGTTTTATTATTAAAAAATAAGAAACTTGTTTTTATTAAACAAGCTAATAATATAAGCGAAAAAGAATTTCAAGAACAATATTAA", "aa": "MELASIFFENKNIILADEPTSNLDFINTKKILKYLQKYVHDNNALLLCNIHDVANSIEYMDYVLLLKNKKLVFIKQANNISEKEFQEQY", "aa_hexdigest": "356e4a14fd902bcc78a7e9be341d4757", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10514.022499999994, "isoelectric_point": 5.591956520080566 }, "id": "IGIGPGHMMC_750", "locus": "GCA000148625_00723" }, { "type": "cds", "sequence": "contig_1", "start": 413012, "stop": 413353, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE5", "UniRef:UniRef90_C4XFE5" ], "nt": "ATGAAAACATACAAAATTGAATTTAAAAATGTAACTTTAGCTTATCAAAATGAAGTGGTCTTAAGCAATTTAAATATCATTTTTGAAAGTGGCAAAACTTATGGAATTTATGGGCTTAGTGGGGTTGGGAAAAGTACTTTGATTAAAGCTATTTTAGAACCAAAATTAATAGCTAAAGGGCAAATATTAATTAATGATCAAGATATCAATAAAATTGATCAAAAGACCTATAAAACAGTTAAAAAAGATATTTCTTTTTTGTTTCAAGAGCCTTCTTTAATTGAGACAGATGATGTCATTGAAAATATTAAAAGAAAGTCAAATAATATCTATAATAATTGA", "aa": "MKTYKIEFKNVTLAYQNEVVLSNLNIIFESGKTYGIYGLSGVGKSTLIKAILEPKLIAKGQILINDQDINKIDQKTYKTVKKDISFLFQEPSLIETDDVIENIKRKSNNIYNN", "aa_hexdigest": "e20d82acec63dbe603376cdd4b243d43", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 412709, "stop": 413362, "observations": { "mutation": [ 413014 ], "stop": [ 413014 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE5", "query_cov": 1.0, "subject_cov": 0.452, "identity": 0.938, "score": 199.0, "evalue": 1.63e-62, "gene_start": 1, "gene_end": 113, "reference_start": 6, "reference_end": 117, "reference_sequence": "MWNNNMKTYKIEFKNVTLAYQNEVVLAFKYHFWSGKTYGIYGLSGVGKSTLIKAILEPKLIAKGQILINDQDINKIDQKTYKTVKKDISFLFQEPSLIETDDVIENIKRKSNNIYNNWFYKVFRILTKEQKNKLAKILEYLDIFDYINTPVKDLSGGQKQRVELASIFFENKNIILADEPTSNLDFINTKKILKYLQKYVHDNNALLLCNIHDVTNSLEYMDYILLLKNKRLVFIKQANNISEKEFQEQY" }, "paralog": false, "identity": 0.9539170506912442, "score": 402.0, "evalue": 4.89e-145, "description": "Internal stop codon at 413014. Nonsense mutation around 413014.", "psc": { "uniref90_id": "UniRef90_C4XFE5", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE5", "UniRef:UniRef50_C4XFE5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE5" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_751", "locus": "GCA000148625_00724" }, { "type": "cds", "sequence": "contig_1", "start": 413433, "stop": 413702, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAAGGATAATTCTTCAAATTATCATATAGCTTTTGATAATGAGAATTCTTTTGCTTATAGTAATCATGGAAATAAAGCATTAGAAAATAGAGATTTTTATGTTAAAGATAAAAATAAAACTTATGAAATGTTAACTTTAACTGATCCTATTCCTTATAATGTGGCTATAGCAAATAATTCACTAAGTAAAAATGTATTAGAATTATTAGCTCAAACTTATATTGAATTAGCAAATAAAAATAATAATTATGTTGGAAATTTATGA", "aa": "MGKDNSSNYHIAFDNENSFAYSNHGNKALENRDFYVKDKNKTYEMLTLTDPIPYNVAIANNSLSKNVLELLAQTYIELANKNNNYVGNL", "aa_hexdigest": "764a55c8c98340f759798a3f323c0fc6", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10138.073699999995, "isoelectric_point": 5.528865242004394 }, "id": "IGIGPGHMMC_752", "locus": "GCA000148625_00725" }, { "type": "cds", "sequence": "contig_1", "start": 414024, "stop": 414209, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATAAAAATAAAAATGTTGTAGCTTTTATGCAAACAAAAACTAGAGCTTTTAAAACAAAAAGTAGTTTAAATTACAATGATCAAAGTCTTTATGATCCAAGTAAAAGTAATAACTTCGCAACTTTACAAGCTCAATATGAAAATGAATTATTCAATAGTGAAGGTGGCTATGTAAATTGA", "aa": "MNNKNKNVVAFMQTKTRAFKTKSSLNYNDQSLYDPSKSNNFATLQAQYENELFNSEGGYVN", "aa_hexdigest": "817667c37054e92ce57c53d97b033df4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7004.609200000002, "isoelectric_point": 9.049507713317869 }, "id": "IGIGPGHMMC_753", "locus": "GCA000148625_00726" }, { "type": "cds", "sequence": "contig_1", "start": 414228, "stop": 414533, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATATATAAATTTATTGTAGCAAGTTCAAGTATTTTTGCTTTAGGAAGTATTAGTGCTGGTTGCCAATATCAACCTAATATTGAAGAAATAAAAATTAAAATGGAAGGTATTAAATATGCAGATAAAGAAAAGTTAGATGCATATACAAAAGTATTTAATCAAGTTTTTAATATGAAAATGAAAGAAGCAAATTTAAATCAAAAAGTAGTTTCGTTTCATGATTCAGGAAATGATGAAAGCGCAACACTTAAAGAAGTTAATTTAAACAAATTTCAAGTGGCTTTTCCCGGAGCTTGA", "aa": "MKKIYKFIVASSSIFALGSISAGCQYQPNIEEIKIKMEGIKYADKEKLDAYTKVFNQVFNMKMKEANLNQKVVSFHDSGNDESATLKEVNLNKFQVAFPGA", "aa_hexdigest": "e3c102f2a7dc05b6a9c1bd8f1049b8e6", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11360.99749999999, "isoelectric_point": 8.968084144592286 }, "id": "IGIGPGHMMC_754", "locus": "GCA000148625_00727" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 414585, "stop": 414686, "strand": "-", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 54.3, "evalue": 2.3e-06, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TGAAATTGACAGAGGTATTTGCTTATATAACAAGCAGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTGAATT", "id": "IGIGPGHMMC_45" }, { "type": "cds", "sequence": "contig_1", "start": 414720, "stop": 414923, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATCACAATTGCTTTGTAACAAGTTTTATCTATTTAATAGCTGCAATATTAATTATTGCAATGCATCATAGTAATATTACTCGTTTAATTAATCATACAGAAAGTAAATTAGGCCAAAAAAAAAGCGAAGATAATTCAGATGATACTGAAGTTGTTTTAATTAGTGAAAAAGAAGAACATGAATCTACTAAATAA", "aa": "MYEYHNCFVTSFIYLIAAILIIAMHHSNITRLINHTESKLGQKKSEDNSDDTEVVLISEKEEHESTK", "aa_hexdigest": "dd1c6d49167307d3b3f504491373fbb0", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7766.662299999998, "isoelectric_point": 5.370454978942871 }, "id": "IGIGPGHMMC_755", "locus": "GCA000148625_00728" }, { "type": "cds", "sequence": "contig_1", "start": 414960, "stop": 415196, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCCACAATCTTTAGCTTTAGGTGTAATCATTGGACATATTTTTCCAGTTTACTTTAAATTTAAAGGTGGTAAAGGTGTAGCTTGTACAATTGGTTTAATTGCCACAATTAATATTGTCTTTTTATTAATGGCTTCTATTATCTTCTTTATAGTTGTCTTAACTTCTAAATATGTATCTTTAGCTTCAATATTAACAGCGGCAATATTAGTGCCCTTTGCTTTTATTCCTTGA", "aa": "MSPQSLALGVIIGHIFPVYFKFKGGKGVACTIGLIATINIVFLLMASIIFFIVVLTSKYVSLASILTAAILVPFAFIP", "aa_hexdigest": "8d66a0f3ff6af79c81325c8b74145ed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8288.133699999991, "isoelectric_point": 9.748087120056148 }, "id": "IGIGPGHMMC_756", "locus": "GCA000148625_00729" }, { "type": "cds", "sequence": "contig_1", "start": 415930, "stop": 416250, "strand": "+", "frame": 1, "gene": null, "product": "Amino acid permease/ SLC12A domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2", "UniRef:UniRef90_C4XFE2" ], "nt": "ATGATTACAGTTATAATAAATTATTCTTTAATTGCTATTTTTGCAATTTTACATAATAGTGGAGTTGTTGAACAAATAATTAAAAATACAATTGATCAAAGAACAAATCAACATTTAGTGCAAAGTTTAAGCATTATTTTTAGTCTATTCTTATTTATTGCTGTTGTTGGAGTATTAAATGATTTTACCAATTCATATGCACATAATATTAATAATGCTATTAATTTAAAAATAATAATTAATACTACTAAAATAAGAAACAAAATGAATCTTAAAAGAATGATGATTTTAATTATAAGTCTTATTTATAGTGCTATTTGA", "aa": "MITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAI", "aa_hexdigest": "298fdedea63d1b0b8f16822e0146dae8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 415693, "stop": 416307, "observations": { "mutation": [ 415804, 416248, 416272 ], "stop": [ 415804, 416248, 416272 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE2", "query_cov": 1.0, "subject_cov": 0.5196078431372549, "identity": 1.0, "score": 187.0, "evalue": 2.13e-58, "gene_start": 1, "gene_end": 106, "reference_start": 80, "reference_end": 185, "reference_sequence": "MINDLNFSGVNLISLYNALSFNKTDQLKNTKTNGISNWATQLGNKKFCYFISFNYTFLSVGSTANNLKKDNLPTVAIVGMITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAIWLIISICIWVLGGDNILDG" }, "paralog": false, "identity": 0.9656862745098039, "score": 375.0, "evalue": 4.63e-135, "description": "Internal stop codon at 415804, 416248, 416272. Nonsense mutation around 415804, 416248, 416272.", "psc": { "uniref90_id": "UniRef90_C4XFE2", "product": "Amino acid permease/ SLC12A domain-containing protein", "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE2", "UniRef:UniRef50_C4XFE2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2" ], "product": "Amino acid permease/ SLC12A domain-containing protein" } }, "id": "IGIGPGHMMC_757", "locus": "GCA000148625_00730" }, { "type": "cds", "sequence": "contig_1", "start": 416953, "stop": 417186, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAAGAAGAATAATAGAAAAACAAATAAAAAAAAATCTTGAAATTTTTGGTGCTATAAACATAGAAGGAATTAAACAATGTGGTAAAACAACTACTGCACAAAAATTTTCAAAAACATTAATTAAATTACAAGATCCAGACAATTTAAAAACTTACAAAGAAACAATTGAATATAAACCTTCATTACTTTTAGAAGGAGAAAAACCATTATTAATAGATGAATGA", "aa": "MKYKRRIIEKQIKKNLEIFGAINIEGIKQCGKTTTAQKFSKTLIKLQDPDNLKTYKETIEYKPSLLLEGEKPLLIDE", "aa_hexdigest": "ce4ef8650e664774e20486f27b1139a7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8968.482799999996, "isoelectric_point": 9.380681800842286 }, "id": "IGIGPGHMMC_758", "locus": "GCA000148625_00731" }, { "type": "cds", "sequence": "contig_1", "start": 417238, "stop": 417765, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAAAGGGGTTATTTATTTTAACAGGCTCAAGTACACACAATAAATTAAATACTATGCATACTGGTACTGGTAGAATACATACAATGAAAATGTATCCAATGAGTTTATATGAAACTGGAGAATCTAGTGGAAAAATATCTTTACAAGATTTATTTGATAAAAAAATTAAAGTAAATGGTTTAAAATCAAATTTATCTTTTGAAGATTTAGCAAAAGCAATTTGCAGAGGAGGATGGCCAAGTACTCTAAATTTAAAAAAATATGAAAATCAAATCGAAATTCCTAAAGATTATGTCAATCAAATTTGCAATGTAGATATAAGTAAAATTGATAACATAAAAAGAAGCCCTGAAATTACTAGAAGAATATTGAAAAGTTATGCAAGAAATGTTGCTACTTTAGCTACTTCAAAAACTTTAATAAAAGATTGTAGTTTTAAAGATGAAGGTTCAAAAACAACTTTTTATGATTACACTACAAAACTTGAAGATTTAAATGTTATTGATAATATAACTGCATGA", "aa": "MGKKGLFILTGSSTHNKLNTMHTGTGRIHTMKMYPMSLYETGESSGKISLQDLFDKKIKVNGLKSNLSFEDLAKAICRGGWPSTLNLKKYENQIEIPKDYVNQICNVDISKIDNIKRSPEITRRILKSYARNVATLATSKTLIKDCSFKDEGSKTTFYDYTTKLEDLNVIDNITA", "aa_hexdigest": "a60c6c17d3e853e2d6fa181ffec30ae7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19724.474400000006, "isoelectric_point": 9.331621360778808 }, "id": "IGIGPGHMMC_759", "locus": "GCA000148625_00732" }, { "type": "cds", "sequence": "contig_1", "start": 417910, "stop": 418218, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGTGTTCTAGAGATTTAAAAATATATTCATCAAGTTTAAATGGAGAATTATCATATTATCACGATAGATATGGTCTTGAAATTGATTTTGTTTTGCACTTAAATGATGGCAGATATGCATTGATAGAATGTAAATTAGGTGGAAATCAAATTGAAAAAGCGAAAAATAATTTATTAAAAGTAAAACAGCTGATAAAAGAAAATGGACAAAGAGAACCGGATCTTATGATTATTTTAACAGCAGAAGATAGAGCGTATGAAATAACAGATGGAATACTTATTATTCCAATTAGTCTACTAAAAGATTAA", "aa": "MCSRDLKIYSSSLNGELSYYHDRYGLEIDFVLHLNDGRYALIECKLGGNQIEKAKNNLLKVKQLIKENGQREPDLMIILTAEDRAYEITDGILIIPISLLKD", "aa_hexdigest": "50c3ca6f07f0e61a10b35c2d9dfeaa70", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_G6AUJ2", "query_cov": 0.9019607843137255, "subject_cov": 0.968421052631579, "identity": 0.522, "score": 95.1, "evalue": 1.46e-23, "valid": false, "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_G6AUJ2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 36, "aa_cov": 0.3431372549019608, "hmm_cov": 1.0, "evalue": 1.3514364354754873e-08, "score": 30.7131404876709, "start": 12, "stop": 46 } ], "seq_stats": { "molecular_weight": 11685.353499999987, "isoelectric_point": 5.352891731262207 }, "id": "IGIGPGHMMC_760", "locus": "GCA000148625_00733" }, { "type": "cds", "sequence": "contig_1", "start": 418251, "stop": 419072, "strand": "-", "frame": 3, "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104", "KEGG:K10563", "RefSeq:WP_013354596.1", "SO:0001217", "UniParc:UPI0001E330D6", "UniRef:UniRef100_UPI0001E330D6", "UniRef:UniRef50_A0A974WKQ3", "UniRef:UniRef90_C4XFD9" ], "nt": "ATGCCAGAATTACCAGAAGTTAGAAGTGTGGTCAAAGATTTAAGACCTAAAGTTGTTAATAGAAAAATAGTTAAAATTGATATCTTACATCCTAAATTAATTAAAGAGGTAAGTGTTGAAGAATTCAAGAATTTTTTAATTAATGAAACATTTTTAGATGTCAATAATTTAGCTAAACATATTATTTTTTCTTTAACAAATAATAAATATTTATTAAGCCACTTGAGAATGAGTGGAAAGTATTTTACACATTATAAATATCGTCCAGCAACAAAACATGATTACTTAATTTTTCATCTTGATGATAATAGTTGTATTTATTATAATGACTCACGTCAATTTGGCACTTTTCATATAAAAACTAAGGGCACTTTATATACAACTAAACCTTTAGATAAAGTTGCAAAAATTCCTTCAGAAACAAATATTAAAGAGTTATTTAATAAAATTAAAAATAAGAATATACCTATAAAACAGCTTCTTTTGGATCAAAGCTTTGTTTCAGGTATTGGAAATATTTATGCTAATGAAACTTTATTTGCCACTCAAATTAATCCTTTGACGCCTTCAAAAAATATAACTTTTGAACAGTTAGAAAAAATTATTAAAGCAGCTGCAAAAATTATGGATCAAGCTACAGAATTAGGTGGTTCAAGCATTGACACATATACTTCAGTTGATGGAGTTAAAGGTCAATTTCAAGATTTTTTACAAGTTCATGGACACTTTAATGATGTATGTAAAAGATGTAAAAAAGCTAAAATTAATAAAATTTTTATTAACAAAAGAGGGACTTATTATTGTCCAAATTGTCAAAAATAA", "aa": "MPELPEVRSVVKDLRPKVVNRKIVKIDILHPKLIKEVSVEEFKNFLINETFLDVNNLAKHIIFSLTNNKYLLSHLRMSGKYFTHYKYRPATKHDYLIFHLDDNSCIYYNDSRQFGTFHIKTKGTLYTTKPLDKVAKIPSETNIKELFNKIKNKNIPIKQLLLDQSFVSGIGNIYANETLFATQINPLTPSKNITFEQLEKIIKAAAKIMDQATELGGSSIDTYTSVDGVKGQFQDFLQVHGHFNDVCKRCKKAKINKIFINKRGTYYCPNCQK", "aa_hexdigest": "c1feeda3eace8e1212ec262cffe91467", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330D6", "ncbi_nrp_id": "WP_013354596.1", "uniref100_id": "UniRef100_UPI0001E330D6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330D6", "RefSeq:WP_013354596.1", "UniRef:UniRef100_UPI0001E330D6" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330D6", "uniref90_id": "UniRef90_C4XFD9" }, "psc": { "uniref90_id": "UniRef90_C4XFD9", "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A974WKQ3", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104" ] }, "pscc": { "uniref50_id": "UniRef50_A0A974WKQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A974WKQ3" ], "product": "Bifunctional DNA-formamidopyrimidine glycosylase/DNA-(Apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "IGIGPGHMMC_761", "locus": "GCA000148625_00734" }, { "type": "cds", "sequence": "contig_1", "start": 419428, "stop": 419748, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAACCCTTTCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTGCTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAACAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAAAATTTCTTCTTCAGTAATATCATCAAAATTAGTCTTTTTCTTAAAAAAATCTTCTAACTAA", "aa": "MRALTLSKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKTDKYPKISLGRLFISFCTFKISSSVISSKLVFFLKKSSN", "aa_hexdigest": "38762aa1748161c12b3b213939d5ee43", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12372.548099999982, "isoelectric_point": 9.913126182556148 }, "id": "IGIGPGHMMC_762", "locus": "GCA000148625_00735" }, { "type": "cds", "sequence": "contig_1", "start": 420993, "stop": 421193, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGCAACCATTTAGTTTGAATATTAGATCCAATTTAAGAATTAAACAAAATGAAAAAAGACTTTTTGTCGATCCTTCTTTAGCAGCTGCGTTATTGAATGTTAATAATGAGAGTTTGCAAAATGATTTAAATACTTTGGGTTTTTTATTTGAAAACCTTGTCTTAAGAGATTTGAAAGTTTATGCTCAAAGTAATGAGTGA", "aa": "MQPFSLNIRSNLRIKQNEKRLFVDPSLAAALLNVNNESLQNDLNTLGFLFENLVLRDLKVYAQSNE", "aa_hexdigest": "62a5f12d2a6b060b2dd6002d34aa8ddc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 0.96875, "evalue": 1.9830703280249298e-12, "score": 43.17341995239258, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7591.5720999999985, "isoelectric_point": 6.178137016296386 }, "id": "IGIGPGHMMC_763", "locus": "GCA000148625_00736" }, { "type": "cds", "sequence": "contig_1", "start": 421470, "stop": 421685, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGGTTCTTCTACTCCTGAACATAAAGGCCTATTTCATAGTGGTATTGGTCGAATTGCTACATTACAATTGAGACCTATGAGCTTATTTGAATCTAAAGATTCTTTAGGACAAATTTCATTAAAAGATTTAATAAATGACAATTTTGAAAATACAACTTATGAAGGTATTGAATTAAATAAAATTATTAATTTAATTATTAGAGGTGGTTGA", "aa": "MTGSSTPEHKGLFHSGIGRIATLQLRPMSLFESKDSLGQISLKDLINDNFENTTYEGIELNKIINLIIRGG", "aa_hexdigest": "e72666246ead2cdd8db9f28b1014b4e9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7831.846499999996, "isoelectric_point": 6.034504890441894 }, "id": "IGIGPGHMMC_764", "locus": "GCA000148625_00737" }, { "type": "cds", "sequence": "contig_1", "start": 421905, "stop": 422006, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATAAAAAAGAAATGTATTATGAAAGATTATTAGATCCAATAATAAGAAAATATTTAAAAGTTTTTGGAGCAATTTGCATTCAAGGACCTAAATGA", "aa": "MTYKKEMYYERLLDPIIRKYLKVFGAICIQGPK", "aa_hexdigest": "4959bb76fbeaae5b28d7f13d39b25b63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4009.8433000000014, "isoelectric_point": 9.517548179626463 }, "id": "IGIGPGHMMC_765", "locus": "GCA000148625_00738" }, { "type": "cds", "sequence": "contig_1", "start": 422189, "stop": 422548, "strand": "-", "frame": 1, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "nt": "ATGATTCAAGATGGAAATAAAGTATTATTTGAAACAATTTTAGTTTTAGATAAACCAAAATACGATATTACTTTTAAAGCTGATAATGAAGATTTAGATAAACTTGTTTATTTAGATAAAAACAAATTGCATAATGTTAACATGACAGCTTTTACAGCAACTTTAGATGCTCACATGAACACAGGTAAAGTTCCAAACATTGTAATGAATTTTGCAAAATTTGATGAATACACATTAGGTTACTTATTCCAATTCTTTGAAAGAGCTTTAGCTATTAGTGCTTATCTTTTAGGTGTTAATCCATTCAACCAACCAGGTGTTGAAGTTTATAAAGCTAATATGTTTAAAATTCTTAAATAA", "aa": "MIQDGNKVLFETILVLDKPKYDITFKADNEDLDKLVYLDKNKLHNVNMTAFTATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILK", "aa_hexdigest": "0ccad641ba841aa1870ecf5ded61c6ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A847B363", "query_cov": 0.9831932773109243, "subject_cov": 0.841726618705036, "identity": 0.513, "score": 111.0, "evalue": 2.88e-29, "valid": false, "product": "glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_A0A971BB97" }, "pscc": { "uniref50_id": "UniRef50_A0A971BB97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "product": "glucose-6-phosphate isomerase" }, "genes": [], "id": "IGIGPGHMMC_766", "locus": "GCA000148625_00739" }, { "type": "cds", "sequence": "contig_1", "start": 422645, "stop": 423193, "strand": "-", "frame": 1, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.9", "GO:0004347", "GO:0006096", "SO:0001217", "UniRef:UniRef50_Q4A5S9", "UniRef:UniRef90_A0A3P8LAA1" ], "nt": "ATGTATGGACCTTACAGCATTAACAAACCAAAAATGGAATTATTATTTGCTGGAAATAGCATTAGTAGTGAAAGCTTAGTAGCAGCATTAAAATATGTTGAAAACAAAAAGTTTGCAATTAATGTTATTTCAAAAAGTGGAACAACTTTAGAACCTTCTATTGCTTTTAGAGAATTTAGAAAATTATTAGAAGCAAAAGTTGGAGCAGTAGAAGCTAAGAATTTAATTGTGGCTACTACTGATGCTAAAAAAGGCGTTTTATACTCATTAGCAACTCAAAAAGGATATGAAAAATTAATTATTCCTGATGATGTTGGTGGACGTTTTAGTGTTTTAAGTCCAGTTGGTTTATTCCCATTCATTTGTGCGGGAATTGATATTGAACAAATTCTTAAAGGTGCTCAAGAAGCTAATAAAGATGCAAATGATTTAAGTTTAAAAACAAATGATGCTTATCGTTATGCGGTTGCAAGACATGTTTTAAGTAAAAATTACACCATGGAAATGATGATTTCTTATGAGCCAAAAATGCAATATTTTGCAGAATGA", "aa": "MYGPYSINKPKMELLFAGNSISSESLVAALKYVENKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLATQKGYEKLIIPDDVGGRFSVLSPVGLFPFICAGIDIEQILKGAQEANKDANDLSLKTNDAYRYAVARHVLSKNYTMEMMISYEPKMQYFAE", "aa_hexdigest": "10efbc395c6977cd16e1c7548848d97a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 422342, "stop": 423469, "observations": { "mutation": [ 422644, 422647, 423274, 423340, 423349 ], "stop": [ 422644, 422647, 423274, 423340, 423349 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LAA1", "query_cov": 1.0, "subject_cov": 0.4262295081967213, "identity": 0.945, "score": 339.0, "evalue": 6.19e-114, "gene_start": 1, "gene_end": 182, "reference_start": 93, "reference_end": 274, "reference_sequence": "MKAVKLNLHNCVDEKLLLSYEDKVKSIHNSIMSKSVVEKDWLGWLELPNKAGSDEVKKMKKIVELWESKNIEAVVVIGIGGSYLGAKSGYEFIYGQYSINKPKMELVFAGNSISSESLVAALKYVEGKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLASQKGYEKLIIPDDVGGRFSVLSPVGLFPFLCAGIDIDQILSGAQAANKDANDLSLKTNDAYRYAVARHILSKNYTMEMMISYEPKMQYFAEWWKQLFAESEGKDGKGLFPTSGIFSTDLHSLGQMIQDGNKVLFETILVLDKPKYDIDFKADKEDLDKLVYLDKNKLHSVNMTAFNATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILKG" }, "paralog": false, "identity": 0.9093333333333333, "score": 667.0, "evalue": 8.38e-246, "description": "Internal stop codon at 422644, 422647, 423274, 423340, 423349. Nonsense mutation around 422644, 422647, 423274, 423340, 423349.", "psc": { "uniref90_id": "UniRef90_A0A3P8LAA1", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_Q4A5S9", "go_ids": [ "GO:0004347", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LAA1", "EC:5.3.1.9", "UniRef:UniRef50_Q4A5S9", "GO:0004347", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5S9" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "IGIGPGHMMC_767", "locus": "GCA000148625_00740" }, { "type": "cds", "sequence": "contig_1", "start": 423347, "stop": 423469, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAGTTAAAGTTAATTTACATAATTGTGTTGATGAAAAAACATTACTTTCTTATCAAGACAAAGTTAAACAAATTCATGATTCAATAATGAACAAAACTATTGCAGAAAAAGATTGA", "aa": "MKAVKVNLHNCVDEKTLLSYQDKVKQIHDSIMNKTIAEKD", "aa_hexdigest": "c5ee275feb6609528749c64ef5df2ee1", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4629.3396999999995, "isoelectric_point": 7.901132392883301 }, "id": "IGIGPGHMMC_768", "locus": "GCA000148625_00741" }, { "type": "cds", "sequence": "contig_1", "start": 423629, "stop": 423994, "strand": "-", "frame": 1, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4", "UniRef:UniRef90_C4XFD4" ], "nt": "TTGAAGCTTTATAGCACAATAAATAATAGTCAATATTTAAAGTCAAATAATGTGACAATGTATGCAATTTTTAGTCAAGATATTTATACTTATGGACAATATTTAATTGATAATAATCGCAACTTTTTTACCTCAATTTTTAGCATTGAAAATGAAAAAATTGACTTTAGAATTAGTGATGAAATTAATAATACTCAAGATGGTTTAGAGAACCATAAAGATAACCGCATAAGCAGCTATTACAAAGTAGCAAAAAAAGGTTATTTGACAACACTTTCAAATGTTGCATCATTACCAGTTTTTGATATTTCATTGGAAAGTTCAAATGAATTAAATTTAGGATACTTAGTTGGATTTCTATATTGA", "aa": "MKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLY", "aa_hexdigest": "cc5ee58e5096f7b663262de3093daad5", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 423578, "stop": 424294, "observations": { "mutation": [ 423631, 424114 ], "stop": [ 423631, 424114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD4", "query_cov": 1.0, "subject_cov": 0.4548872180451128, "identity": 0.992, "score": 236.0, "evalue": 9.96e-77, "gene_start": 1, "gene_end": 121, "reference_start": 129, "reference_end": 249, "reference_sequence": "MNFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTNWLSSNLEQNKAFQYAYIRYSLKGDRKNDLLIGTDERFKGILKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLYWSYIFECYLNKLNPFDI" }, "paralog": false, "identity": 0.9915966386554622, "score": 466.0, "evalue": 6.38e-170, "description": "Internal stop codon at 423631, 424114. Nonsense mutation around 423631, 424114.", "psc": { "uniref90_id": "UniRef90_C4XFD4", "product": "Glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD4", "UniRef:UniRef50_C4XFD4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "IGIGPGHMMC_769", "locus": "GCA000148625_00742" }, { "type": "cds", "sequence": "contig_1", "start": 424112, "stop": 424822, "strand": "-", "frame": 1, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHM8", "UniRef:UniRef90_C4XFD3" ], "nt": "ATGGCAACAATTAATTTGAAAGCTTATAATTATTCACCTGATTTTAAAAATGAAAGTTTGATTAATCGTGGCAATAACTTGATTAAGGATGTTAAATTAAAAAATATCGAGGGTTTTGAACATTTTGGTTTTCATGAATTAGCTTTAAATTTTAGTAATTTTAATGTTGATGAATTAAACAATTTTGTACGCTTAATAAAAAAGCAAGAAATTAAAGAAATTTTGATTATGACAACAAGCAAAATAATTGATAATTATCAAGCTGGAAATGACTTTTTATTTAAGTATGATTTATTAAAAAAATACAAGTTAAAATTTAATTTTATTAATGTTGATAATTATGAGCAAGATTACTATGCTAAGTTTGAAGAGTATAAGAAAAAATTTGAACAAAAAGGCATTGCTTTAATGTTTGGATCATTTACTAAATTTAGTGAAGAAACAATTAATTTTATTAAATTATTAATGAATCATTTTCAATTAAATCAAGGTTATTATCGAGCACTTGAAAGAGTTTTTTTAATCTCAAAAGAATCATTAGAAAAACAATTGGAATTTTTAAGTATTCCCCTTCAAAACAGACTTATTAGTCCAAATATCCTCTCAAATAATTTTGCTTTTTTTAGTGAAATTAATATGTTACTTTTGTTGCTTCAAGGAGCTAATATTAATGACTTATCTGAAGGCTATTCTTCAGGTTGCACAAACTGA", "aa": "MATINLKAYNYSPDFKNESLINRGNNLIKDVKLKNIEGFEHFGFHELALNFSNFNVDELNNFVRLIKKQEIKEILIMTTSKIIDNYQAGNDFLFKYDLLKKYKLKFNFINVDNYEQDYYAKFEEYKKKFEQKGIALMFGSFTKFSEETINFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTN", "aa_hexdigest": "043d74a416c8e1991b8d051ddb05fc79", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 423809, "stop": 424822, "observations": { "mutation": [ 424114 ], "stop": [ 424114 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD3", "query_cov": 1.0, "subject_cov": 0.5700483091787439, "identity": 1.0, "score": 464.0, "evalue": 1.58e-162, "gene_start": 1, "gene_end": 236, "reference_start": 1, "reference_end": 236, "reference_sequence": "MATINLKAYNYSPDFKNESLINRGNNLIKDVKLKNIEGFEHFGFHELALNFSNFNVDELNNFVRLIKKQEIKEILIMTTSKIIDNYQAGNDFLFKYDLLKKYKLKFNFINVDNYEQDYYAKFEEYKKKFEQKGIALMFGSFTKFSEETINFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTNWLSSNLEQNKAFQYAYIRYSLKGDRKNDLLIGTDERFKGILKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLYWSYIFECYLNKLNPFDI" }, "paralog": false, "identity": 0.9970326409495549, "score": 667.0, "evalue": 4.37e-245, "description": "Internal stop codon at 424114. Nonsense mutation around 424114.", "psc": { "uniref90_id": "UniRef90_C4XFD3", "uniref50_id": "UniRef50_A0A3P8LHM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD3", "UniRef:UniRef50_A0A3P8LHM8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHM8" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "IGIGPGHMMC_770", "locus": "GCA000148625_00743" }, { "type": "cds", "sequence": "contig_1", "start": 424809, "stop": 425036, "strand": "-", "frame": 3, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGCGTCATGAATTTAAGATCGGCGAAAAAATTAATTTTATAGTTGAAAAAGTTGATTATCACAGTCATAGTGGTATTGGTAATTTTAAAATTAATCATGCCACCTTTTCACGCAGTCCATTTAAAGAAAATTTAAGCAATACAAAACATGGTTTTGAAAACTTGAAAAACCAAATTGATCAAGAAATCAAAAATTATCATAAAGAAGGTAATAATGGCAACAATTAA", "aa": "MRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN", "aa_hexdigest": "8887cc25eed6748d55227d3c5a208f77", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 424809, "stop": 425315, "observations": { "mutation": [ 425051 ], "stop": [ 425051 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 0.987, "score": 149.0, "evalue": 1.53e-44, "gene_start": 1, "gene_end": 75, "reference_start": 94, "reference_end": 168, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.41e-122, "description": "Internal stop codon at 425051. Nonsense mutation around 425051.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "IGIGPGHMMC_771", "locus": "GCA000148625_00744" }, { "type": "cds", "sequence": "contig_1", "start": 425049, "stop": 425273, "strand": "-", "frame": 3, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGACCAAAAAAATTGCTAAAAAATCAAATATTTTTGCATTTTTTCTTATTTTTTTCCATTTAATTATTAATATAAACATAAAGTTTAAGTATAATAAAAACACCATGAATCATAAAGGCGATATTTTATTCGGAAAAATAAAAAGTGTAAGTAGTGAAGGACTTACAATTTTATGCAATAATGATTATTCTTATTTTATTCCTCGTGATTTGATAACTGACTGA", "aa": "MTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITD", "aa_hexdigest": "3302a32d0eb16ac32954932f9fb56dd5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 424809, "stop": 425315, "observations": { "mutation": [ 425051 ], "stop": [ 425051 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44047619047619047, "identity": 0.986, "score": 149.0, "evalue": 1.03e-44, "gene_start": 1, "gene_end": 74, "reference_start": 15, "reference_end": 88, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.35e-122, "description": "Internal stop codon at 425051. Nonsense mutation around 425051.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "IGIGPGHMMC_772", "locus": "GCA000148625_00745" }, { "type": "cds", "sequence": "contig_1", "start": 426014, "stop": 426433, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTCAATGAAATGGCTCGTAAAATGGAACTTTTAAGACAAAACTACAAGCCACTTGAAGGTGTAACATACATGAACTACATCACATTAAGTTTTGATGACTATGTAGGTGGTGTTGCTTTATACACAGAAAAAATTAAAAATATTATTGAAACAACTCCAAAACAAAATATTAGTGAAGCTGATTACAATAATATTAAGAAAAACTATGCTAACTTCAATAAACAATTATTAGAAGTTGAAGCAAATCCTAACTTCCCACATGCTGATTTAGTTCAAAAAATGCATGCTGAATTAAGTACAACTTTTGAATCATTACTTAGTTTATTAGTCTTAGATCCTAAATTAGAAGATACACCAGCTAACCGCGGTTATTTCCTAAATAAAGAAACTAAGCAAAAACAAGAAATCTCATGA", "aa": "MNFNEMARKMELLRQNYKPLEGVTYMNYITLSFDDYVGGVALYTEKIKNIIETTPKQNISEADYNNIKKNYANFNKQLLEVEANPNFPHADLVQKMHAELSTTFESLLSLLVLDPKLEDTPANRGYFLNKETKQKQEIS", "aa_hexdigest": "573070d8fb7684ef898c1a37d418e986", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16130.149199999983, "isoelectric_point": 5.434739875793456 }, "id": "IGIGPGHMMC_773", "locus": "GCA000148625_00746" }, { "type": "cds", "sequence": "contig_1", "start": 426608, "stop": 427177, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGTTTTAGCTGCTGAAAAATCTTCTAAAACTAAAAAACAACCTAAAACTGAAACTATAGTTACTGAAGTGCCTCCAGTAGTTGAAGAAGTTAAAGATGAATCAGTTGTTGAAGCTGAAGCTGTAGTTGAAGAAGTTAAACCAGTTGAAGAAAAACAACCAACAATTAAAGAAGAACCGCTTATTGTTTTAGCTCCTGAAGAAGCTAAAAAAGAAAAAGTTGAAGACAATGATTTTGTCTTAATTGACCATAAAAAAGCTGAAAAACCTAAAACAAGAGACTTTGCTGTTAGAGTTGTTGAAGAAAAACCTATTCAAGTAATTGAAGAAAAAGAAGCTCAACCTGAAGATAATTTCTACACAGGAATTAGAGGTAATGAACCAGTTGCTCTAGAATCAGAAGAATCATCAAACAATGTTTCAACTAAGAAAAAACGTTCAGCAGGATTCTACTTCTTAATCTTATTATTAGTACTAATTCTTGCAGGTCTTTTAACCTTAGATATTCTTGCTATTATTCAATTAGCAGGTGGTTATACATTCTTTAATTTTGGTGCTTAA", "aa": "MEEAVLAAEKSSKTKKQPKTETIVTEVPPVVEEVKDESVVEAEAVVEEVKPVEEKQPTIKEEPLIVLAPEEAKKEKVEDNDFVLIDHKKAEKPKTRDFAVRVVEEKPIQVIEEKEAQPEDNFYTGIRGNEPVALESEESSNNVSTKKKRSAGFYFLILLLVLILAGLLTLDILAIIQLAGGYTFFNFGA", "aa_hexdigest": "bcf4e3f85d9e51ff9a607d5f6a1ffabb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21064.854000000007, "isoelectric_point": 4.723740959167479 }, "id": "IGIGPGHMMC_774", "locus": "GCA000148625_00747" }, { "type": "cds", "sequence": "contig_1", "start": 427257, "stop": 427361, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATAATGAAAAAAATATGACTACTTTAATAAATCATTTAAAAGCTAGTGGATTTGTTTATCAAGGTAGTGAAATTTATGGTGGTTTAGCTAATACTTGA", "aa": "MKNNEKNMTTLINHLKASGFVYQGSEIYGGLANT", "aa_hexdigest": "051db83d179e4d9698cbf2e553135eee", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.2002, "isoelectric_point": 8.161391258239746 }, "id": "IGIGPGHMMC_775", "locus": "GCA000148625_00748" }, { "type": "cds", "sequence": "contig_1", "start": 427704, "stop": 428108, "strand": "+", "frame": 3, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "ATGTTTGAAACCAAACAAGGAGTTACAGAAGAAACCAAAAACAAAATTTACTTACGTCCTGAAACTGCTCAAGGTATTTTTGTTAACTTTAAAAACGTACAAAGAAGCATGCGTTCAAAATTACCTATGGGCATAGGGCAAGTTGGAAAAAGTTTCAGAAATGAAGTAACACCAGGTAACTTTATTTTCCGTACTCGTGAATTTGAACAAATGGAACTTGAAGTATTTTGCCATCCAAAAGAAGCAAACAAACTTTACAAATACTACATTAAAAAAGCTGAAAAATTTGCTGAATATTTAGGCTTAAGCAAAGAAAATGTTCGTATCAGAGAACATGAAAAAGAAGAACTAAGTCATTATTCTTCAGGCACAAGTGACATTGAATATCTTTTCCCTTTTGGCTGA", "aa": "MFETKQGVTEETKNKIYLRPETAQGIFVNFKNVQRSMRSKLPMGIGQVGKSFRNEVTPGNFIFRTREFEQMELEVFCHPKEANKLYKYYIKKAEKFAEYLGLSKENVRIREHEKEELSHYSSGTSDIEYLFPFG", "aa_hexdigest": "8d4028caa58c5e3eefa13a1b12699004", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8582089552238806, "subject_cov": 0.8273381294964028, "identity": 0.6, "score": 145.0, "evalue": 3.18e-42, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "IGIGPGHMMC_776", "locus": "GCA000148625_00749" }, { "type": "cds", "sequence": "contig_1", "start": 428160, "stop": 428597, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGAAAGCTACTGGTGAAAACTTAGAATATGTTGACCCTGTAACAAACGAAAAATTTGTTCCTTTTGTTATTGAACCTTCAATAGGACTTGACCGTTTAATGTTAGCAATTTTAATTGATTCATATGACGAAGAAAAACTTGAAAAAGATGACTCAAGAGTTGTTTTACATCTACACAAAAATTTAGCTCCTTATCAAGTTGCAGTTTTACCTTTAGTTAAAAAACTAAATGATAAAGCAACAGAAGTTTATGAAAAATTACTAAAAGCAGGCTTATCTGTAACTTATGATGAAACTGGATCAATAGGCAAAAGATACCGTCGTCAAGATGCTATTGGTACACCTTATTGTGTTACAATAGACTTTGATTCACTTGAAAAAAATACTGTAACAATTAGAGACAGAGACAGTATGAAACAAAAAAACTGTACTAATTAA", "aa": "MKATGENLEYVDPVTNEKFVPFVIEPSIGLDRLMLAILIDSYDEEKLEKDDSRVVLHLHKNLAPYQVAVLPLVKKLNDKATEVYEKLLKAGLSVTYDETGSIGKRYRRQDAIGTPYCVTIDFDSLEKNTVTIRDRDSMKQKNCTN", "aa_hexdigest": "e62e5a1f754a31ef7622ede2d7d677ab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.9724137931034482, "subject_cov": 0.8245614035087719, "identity": 0.596, "score": 170.0, "evalue": 1.74e-51, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 81, "aa_cov": 0.5310344827586206, "hmm_cov": 1.0, "evalue": 3.267532652824063e-25, "score": 84.15796661376953, "start": 66, "stop": 142 } ], "seq_stats": { "molecular_weight": 16510.71939999999, "isoelectric_point": 5.456338691711425 }, "id": "IGIGPGHMMC_777", "locus": "GCA000148625_00750" }, { "type": "cds", "sequence": "contig_1", "start": 428653, "stop": 428889, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGAGTAATAATAACCATGAAATAGTTGACTCAATTATTGCAAGTAATGATATTGTTGATGTTATCAACTCATTTGTTACTTTAAGCAAAAAAGGTCGTAGTTTTGTTTGCATTTGTCCTTTTCACCAAGACACAAATCCATCAATGAATGTCGACCCTAAAAAGCAAATATTTAAATGCTTTGTCTGCCAAGTTGGTGGTAATGTTTTAACATTTTTAATGAAATACAAAAAATGA", "aa": "MSNNNHEIVDSIIASNDIVDVINSFVTLSKKGRSFVCICPFHQDTNPSMNVDPKKQIFKCFVCQVGGNVLTFLMKYKK", "aa_hexdigest": "279c97a7e20988a35d5eda3ed4f4a468", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 73, "aa_cov": 0.9358974358974359, "hmm_cov": 1.0, "evalue": 1.206719720458388e-28, "score": 94.85169219970703, "start": 6, "stop": 78 } ], "seq_stats": { "molecular_weight": 8798.178199999998, "isoelectric_point": 8.781061363220214 }, "id": "IGIGPGHMMC_778", "locus": "GCA000148625_00751" }, { "type": "cds", "sequence": "contig_1", "start": 429490, "stop": 430125, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13662.12" ], "nt": "ATGGGTACAGCTTTAACCAATAATCATTTCCGATTACTAAAAAACAAAAAAGTAGTCATCTTTTTAGATGGTGATGATGCTGGTCAAAAAGCTTCACTAAAAAGTGCTAAGTTTTTATTAGACCATCGTATTGACACTTACATAGTACGTAACACAACTAAATATGACCCCGATGAATTATTTAATAGTTATGGTGCCGATATTCTTCACAAGTTAATTAAAGAAGCTCCAGCTGCATTAGATTTTATTTATGAAAATTTAAGCAAGAAATATAATCTCAGTGTTTCGCCAAACAATGAAATCAAAAATGTTAAAGCTTTTTGCAATGACTTTGTTAACTTCCTTGAACACCAAGAAATTAATGTTCAAGAATTCTACAAAAACAAAATTCAAACCGATTATGGTTTTAGCATTCAAGTAAAACACAACTTTAAGTTGCCTGATCCTTATGATTCTCAAGAAATTTATGCACCACAACAATTTCCAGAAGACTCAGGTGAATATCAATTATTAGAAAACAATATTCCCCCTTATGCTTATGATGAACAAAATTATAATCAAGTAGTTGAATACAGCAATAATGTCAATAATTTTAATCACCCTTTAAATAACGAAGTGCCATTAAGCGATTATTGA", "aa": "MGTALTNNHFRLLKNKKVVIFLDGDDAGQKASLKSAKFLLDHRIDTYIVRNTTKYDPDELFNSYGADILHKLIKEAPAALDFIYENLSKKYNLSVSPNNEIKNVKAFCNDFVNFLEHQEINVQEFYKNKIQTDYGFSIQVKHNFKLPDPYDSQEIYAPQQFPEDSGEYQLLENNIPPYAYDEQNYNQVVEYSNNVNNFNHPLNNEVPLSDY", "aa_hexdigest": "b73073e19d5197eec1d296505d8a2b58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13662.12", "name": "Toprim_4", "length": 56, "aa_cov": 0.22274881516587677, "hmm_cov": 1.0, "evalue": 2.2547645093478013e-07, "score": 27.045278549194336, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 24562.979999999996, "isoelectric_point": 5.014642906188963 }, "id": "IGIGPGHMMC_779", "locus": "GCA000148625_00752" }, { "type": "cds", "sequence": "contig_1", "start": 430210, "stop": 430560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTCGGTAAATTAAATAATGAAGATTTAAAAAAAGAACTTTATCAAACATTTAGAGAAAGTAAAAGTATTAAGGTTGCTAAATTAAAGGAATATATCAAAGACTTAGAACAGGAAAATGTTGATATCGATTTAAATCAATTTTCAAAAAAATACAAAGAGGATAAAATTATTAAACAAAACTTTAAAGATGTTTATCGCCGAGCATTAAAGGAATCAGATAATGCTTATAATAAATACACAAGCAATCCTGAAGTTTTGAAACAAATCAGCAGTATCAAAAAAATGTATGATGAACACAAAGAAAAATTAAGAGAAATGCAAAAAAGAAAGGAAGAAAATGAATAA", "aa": "MFFGKLNNEDLKKELYQTFRESKSIKVAKLKEYIKDLEQENVDIDLNQFSKKYKEDKIIKQNFKDVYRRALKESDNAYNKYTSNPEVLKQISSIKKMYDEHKEKLREMQKRKEENE", "aa_hexdigest": "9d84694ae9e99a902a594380dcf948f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14195.017899999986, "isoelectric_point": 9.18708324432373 }, "id": "IGIGPGHMMC_780", "locus": "GCA000148625_00753" }, { "type": "cds", "sequence": "contig_1", "start": 430553, "stop": 431176, "strand": "+", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8", "UniRef:UniRef90_C4XFC8" ], "nt": "ATGAATAAAGCAAATGAAATTAAATCAATAATCTCAATGATTAAAAAACATGCAAAAAGCTTAAAGAAAAAACAAATAAGTCAGTCTAAAGTTTATGATTATTTAGATTCAATTAAAGTTGAAATTGATGATGAATCAATGGACGAAGTTTTAGGCCAACTTTTTGAATCAGGCTTAATTCTTGATGAAAGCGACGAGGGCGATTTAGACGATGTTAGTTTAGATGAATTAGACTCAGAATTAGAATTCGAAGAAAAAAGTAAAAAAGACGAAAACACCGATGAAATGGATGTTAAAAACAAAAAAAGTGATTTATATGACGAAGATTTAGATGAAGATGATAACTACAATTTAGTTGGTTCATATGGTGACGATGATGAAAGTCAAGAATACATTATTGAAGATGACTATGTTGATTCAAGTTATGATGAAGATGACGAAGATGAAGACGAAGAAGAATTAAGCAGTAAGAAAAAATCGAAAAAAAGTAAAAATAATGAAGATGAAGATTCACTTGAACTTGAATCTTATGAAAATGAAGAAATTAACCTTAATGATAACAAAGTAATTAAAGATAAAGATTTAACCAACAAACTTACCGAAACTAATGATATTGTTAAATGA", "aa": "MNKANEIKSIISMIKKHAKSLKKKQISQSKVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVK", "aa_hexdigest": "ab3150d293625c8080a58341d6127922", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 430553, "stop": 431479, "observations": { "mutation": [ 431174, 431186, 431450, 431453 ], "stop": [ 431174, 431186, 431450, 431453 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC8", "query_cov": 1.0, "subject_cov": 0.4233128834355828, "identity": 0.995, "score": 306.0, "evalue": 5.56e-100, "gene_start": 1, "gene_end": 207, "reference_start": 1, "reference_end": 207, "reference_sequence": "MNKANEIKSIISMIKKHAKSLKKKQISQSEVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVKWYMRWIGKYGKLLTIEEEKKLAQEMEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYATWWIRQAITRAVADQARTIRVPVHMVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK" }, "paralog": false, "identity": 0.9837662337662337, "score": 523.0, "evalue": 1.54e-187, "description": "Internal stop codon at 431174, 431186, 431450, 431453. Nonsense mutation around 431174, 431186, 431450, 431453.", "psc": { "uniref90_id": "UniRef90_C4XFC8", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC8", "UniRef:UniRef50_C4XFC8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8" ], "product": "RNA polymerase sigma factor" } }, "id": "IGIGPGHMMC_781", "locus": "GCA000148625_00754" }, { "type": "cds", "sequence": "contig_1", "start": 431246, "stop": 431452, "strand": "+", "frame": 2, "gene": null, "product": "sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6", "UniRef:UniRef90_UPI0021E0C4F6" ], "nt": "ATGGAAAAAGGTGGCTTTAGAGGAAAACGTGCTAGGGATAAACTTATCAAACGTAATTTACGTTTAGTTATCAATAATGCTAAAAAATACAAAAATAGAGGACTTAGCTTTATTGACTTAATCTCAGAAGGTAACTCAGGTATCGTAAAAGCAGTTTCAAAATATAACGTTTCAAAAGGCTTTAAATTCTCAACATATGCAACTTGA", "aa": "MEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYAT", "aa_hexdigest": "ed6a4481227ac60f778d6860788d7ef0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 431200, "stop": 431524, "observations": { "insertion": [ 431242 ], "mutation": [ 431218 ], "stop": [ 431218, 431450, 431453 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "query_cov": 1.0, "subject_cov": 0.591304347826087, "identity": 0.868, "score": 117.0, "evalue": 7.01e-33, "gene_start": 1, "gene_end": 68, "reference_start": 19, "reference_end": 86, "reference_sequence": "MERWKTSRWCWRKKLCNEIEKGGFRGKRARDKLVLRNLRLVINNAKKYKNRGLSFIDLISEGNQGIMKAVQKFDVSKGYKFSTYATWWIRQAITRAVADQARTIRVYLYIYRNNK" }, "paralog": false, "identity": 0.7850467289719626, "score": 159.0, "evalue": 1.16e-51, "description": "Internal stop codon at 431218, 431450, 431453. Frameshift due to insertion around 431242. Nonsense mutation around 431218.", "psc": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021E0C4F6", "UniRef:UniRef50_UPI0021E0C4F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6" ], "product": "sigma-70 family RNA polymerase sigma factor" } }, "id": "IGIGPGHMMC_782", "locus": "GCA000148625_00755" }, { "type": "cds", "sequence": "contig_1", "start": 431519, "stop": 432022, "strand": "+", "frame": 2, "gene": null, "product": "sigma-70 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "nt": "ATGGTTGAAACTATTAACAAAATCACTAAAATTGAACGTGAATTGCAACAAGAATTAGGTTATGAACCAACTGATGAAATGATTGCTGAAAGATATGGCAATGGCTATGACGCTGAAAAAGTTAGATACATTAGAAAAATCAACATTGACCCTATTTCTTTAGACAAACAAATTGGTAAAGAAAATGATTCATCATTTAGTGACTTTGTTAAAGATGAAAGTGTTGTTAATCCAGTTGATTATGCTTCACAAGAAGAATTAGTGGAAACACTAAATTCAATTCTTGAAAAATCACTTGATGATGATGAAAGAATTTTAATCTGCAAACGTTTCGGAGTTGGAGTTGACGAAAATGGAAATAAATACCCAATTCACTCACTTGAAAAATTAGCAGAAGAAAGAAATGTTTCTAAAGAAAGAATTCGTCAAATTGAAAACAAAATTTTAAGAAAACTTAAAAGTAGCACACGTAAAGGACGTCACTTGAAAGATTTCATTAAATAA", "aa": "MVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK", "aa_hexdigest": "d8910b176a042d5196fe307d3e10f9a7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF345E0", "query_cov": 0.8922155688622755, "subject_cov": 0.9933333333333333, "identity": 0.591, "score": 181.0, "evalue": 8.13e-56, "valid": false, "product": "sigma-70 domain-containing protein", "uniref50_id": "UniRef50_UPI002FF345E0" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF345E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "product": "sigma-70 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_783", "locus": "GCA000148625_00756" }, { "type": "cds", "sequence": "contig_1", "start": 432015, "stop": 432212, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTAAATTAGCATCTATTTCTAAAGCTTTTTCGAAAACTTCTTTGGTTAAGTCAATAGCACAAACTATACTACGTAAAGGTTCTGATAAATTGAATTTAATAGAAAAACCACTTGGGTCTCATTCTTCTTTAAGATCAAGTGGTCACTTTTCTAATAAATATTTTGTTACTTTTCTAATTTGCATTATTTAA", "aa": "MMIKLASISKAFSKTSLVKSIAQTILRKGSDKLNLIEKPLGSHSSLRSSGHFSNKYFVTFLICII", "aa_hexdigest": "e26b4431c9406e3ce81c146386714cbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.508499999996, "isoelectric_point": 10.302902030944821 }, "id": "IGIGPGHMMC_784", "locus": "GCA000148625_00757" }, { "type": "cds", "sequence": "contig_1", "start": 432874, "stop": 433032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGAAAAAATCCTAAAAGTTCAGCTATTGCTTTGATTGTTTTATCACTTTTAAGCGCTGTTATAACAGGAATTGCAATATTTATGTTTATTGATGTTGCAGTTAAAATATTTAAAAGTGGAGAAGAAACAAATGAATATATTAAAAATAACTGA", "aa": "MHRKNPKSSAIALIVLSLLSAVITGIAIFMFIDVAVKIFKSGEETNEYIKNN", "aa_hexdigest": "9c018b39b53836b8fc329e9044cc8e2f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5736.745, "isoelectric_point": 9.302868461608885 }, "id": "IGIGPGHMMC_785", "locus": "GCA000148625_00758" }, { "type": "cds", "sequence": "contig_1", "start": 433150, "stop": 433293, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATCTTAATTATAGGATTCTTCGTTGGAATATTAACATTCATCGGAGCATTTATTTATATTTCTGATGCTCAAAAAGAAAATAAAAACTACTTCAATTATTTTAAATCAGATGAATCAATTGACTCAAAAGCAGAATAG", "aa": "MIILIIGFFVGILTFIGAFIYISDAQKENKNYFNYFKSDESIDSKAE", "aa_hexdigest": "33a6840712a18ef3e6ea85f4642cdb96", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5415.169400000001, "isoelectric_point": 4.748352241516113 }, "id": "IGIGPGHMMC_786", "locus": "GCA000148625_00759" }, { "type": "cds", "sequence": "contig_1", "start": 433407, "stop": 433505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGGAGATCTATGTCAACAGAAAACAAAACAGAATCTTTAAACAAAGTAAACAATGAAAAATTGGAAGCAAAAGAATTAAGCTATACTAAATGA", "aa": "MKRRSMSTENKTESLNKVNNEKLEAKELSYTK", "aa_hexdigest": "5d5edf307d0e1169c7525b30cfa8f4ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3788.2687000000014, "isoelectric_point": 9.600390052795408 }, "id": "IGIGPGHMMC_787", "locus": "GCA000148625_00760" }, { "type": "cds", "sequence": "contig_1", "start": 434098, "stop": 434652, "strand": "+", "frame": 1, "gene": null, "product": "HEAT repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4", "UniRef:UniRef90_C4XFC4" ], "nt": "ATGGATGAAGAAAGTGTAGATGCATTATTAAAACGATTAGAAAAAGAAGACAAACTTTATACTAAAATCGCTATTTGTGAAACTTTAGAAACAGGTAATGCTTTTACAGCTAATAAAATGATTGAATATTTGAGCAAAATTGGTAATAACCAAATTAAAGAATTGCCTAAATATATTAGTAATAAAAGTTCTTATCCACTACCAAGAGACATTATTGCCAAATCTTTAGCTAAAATGCATACTTCAATTTTTCCTCTTTTACTTGAAACTCTTAAGCAAGGAACAATTGAACAAAAACAAGAAGTTATTGATGCAATTGGTTACATTTGTTTTAATAAAAAAGAATTAGCTACTGAAAAACTTTTAAATGAGCTAATTGATGTTTATGGTCAAAATATTGATAATAAAATTTTTGTTTGGAAATTTTTAACATTATTATCAGCTTTTAATTTTGAAGCAAGTAAAGAAATACTTAACTCATATTTAAATAGCAAAGATACTTTATCAAAAGAAGCAACTCACTCTTTAGATATATTAAAGGAAGCAAATATTTAG", "aa": "MDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI", "aa_hexdigest": "0537e01ee56c60b62ad7e6c83d2e8095", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 433897, "stop": 434652, "observations": { "mutation": [ 433966, 434062 ], "stop": [ 433966, 434062 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC4", "query_cov": 1.0, "subject_cov": 0.7330677290836654, "identity": 1.0, "score": 356.0, "evalue": 3.78e-123, "gene_start": 1, "gene_end": 184, "reference_start": 68, "reference_end": 251, "reference_sequence": "MSQIKAHFYIKRILMKPVENKKQWLSRGYVDQEDIAEFENKSKKELLELLQHEYAWVRSSAAYNLSPMDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI" }, "paralog": false, "identity": 0.9920318725099602, "score": 482.0, "evalue": 2.39e-175, "description": "Internal stop codon at 433966, 434062. Nonsense mutation around 433966, 434062.", "psc": { "uniref90_id": "UniRef90_C4XFC4", "product": "HEAT repeat domain-containing protein", "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC4", "UniRef:UniRef50_C4XFC4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4" ], "product": "HEAT repeat domain-containing protein" } }, "id": "IGIGPGHMMC_788", "locus": "GCA000148625_00761" }, { "type": "cds", "sequence": "contig_1", "start": 434702, "stop": 434887, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "TTGAAACATGAATTTAACAAAAATATTTACAATGCAAAAAGATCTGACAAAGCTATTGCAGCTCGTAATGATATTGATGCAAAGACTTCAAAAAGCAAAATTAAAGAATTTCACTACATGAAATTATTAGCTTTACTTGTTGAAATGGCAGAATTTGCAAATGAAGTCCAAACATTCAAATATTGA", "aa": "MKHEFNKNIYNAKRSDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVEMAEFANEVQTFKY", "aa_hexdigest": "5bb8ee9fbad2b57e716a8cf1f203d2e3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 41, "aa_cov": 0.6065573770491803, "hmm_cov": 1.0, "evalue": 3.579746162019693e-08, "score": 29.892457962036133, "start": 25, "stop": 61 } ], "seq_stats": { "molecular_weight": 7183.251199999997, "isoelectric_point": 9.476739692687989 }, "id": "IGIGPGHMMC_789", "locus": "GCA000148625_00762" }, { "type": "cds", "sequence": "contig_1", "start": 434942, "stop": 435211, "strand": "+", "frame": 2, "gene": null, "product": "dUTPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33", "UniRef:UniRef90_C4XFC3" ], "nt": "TTGCACTTTGTTGGCTCTTTTGCTTATCAATATGAAGTTGATCCTAACATCACACCATTAATAATGAGTAAGGATGTTAATAAACAAATTTGCTTATTATTCCAAACAATTTCTTCAGCAACAAATAACATCAATAAATATGTTATTGGAGAAATATTAGCTTTAACTTTAGGGGTTGCTAAATTATTAGGTTACAGTCAAAAAGATCTTTTAGAAGCTTATTACAACAAAAACAAAAAGAATTACGAACGCATTAAAAATAAATATTAG", "aa": "MHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY", "aa_hexdigest": "36cb5dfe1e264b4a6a1c29ae44be49fc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 434676, "stop": 435211, "observations": { "deletion": [ 434748 ], "mutation": [ 434703 ], "stop": [ 434703, 434886 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC3", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.9890000000000001, "score": 175.0, "evalue": 1.7e-54, "gene_start": 1, "gene_end": 89, "reference_start": 90, "reference_end": 178, "reference_sequence": "MALLNLLYNWNMNLTKIFTMQKDLDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVETAEFANEVQTFKYWKAKKQIDKDKALEEFADILHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY" }, "paralog": false, "identity": 0.9775280898876404, "score": 333.0, "evalue": 3.75e-119, "description": "Internal stop codon at 434703, 434886. Frameshift due to deletion around 434748. Nonsense mutation around 434703.", "psc": { "uniref90_id": "UniRef90_C4XFC3", "product": "dUTPase", "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC3", "UniRef:UniRef50_N9VF33" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33" ], "product": "dUTP diphosphatase" } }, "id": "IGIGPGHMMC_790", "locus": "GCA000148625_00763" }, { "type": "cds", "sequence": "contig_1", "start": 435713, "stop": 437116, "strand": "-", "frame": 1, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "nt": "ATGTCCTTATTTAAAGAAGATGAAAAAAATGCTAAATTACAAACAGAATTTAACAATGTTCCTATTCTTACTTTAGTTGGTCCTCCAGGTACTGGTAAAACCTCTTTAGCCAGAGCTATTGCTGAAGCGCTTAACAAAAGTTACATTAAAATTAGTCTTGGTGGTGTGCATGATGAAAGTGAAATTAGAGGTCACAGACGTACTTATGTAGGTTCAATGCCAGGTAAAATTATTAAAGCTATCCAAAGAGCTGGTGTATCTAACCCACTTATTTTATTGGATGAAATTGACAAAATGTCATCTGATTATAAAGGTGATCCAGCTAGTGCAATGCTTGAAGTATTAGATCCTGAACAAAACACAAAATTCCAAGATAACTATCTTGAACATGAATATGACTTATCAAAAGTTATGTTTATTGCAACTGCTAACTACTACGAAAATATTCCTGCTCCTTTAATTGACCGTGTGGAAATTATCGAATTAAACTCATATACAATTAATGAAAAAATTAAAATTGCACGTGAACACTTAATTGATTCTACAATTCAACAAGCAGGTCTTAAAAAAGAACAATTCAATATTGATGATAAAACACTAGAATTTATTATCAAACACTATACAGCTGAAGCTGGTGTTCGTGGACTTAAGAGAGATTTAGATAAAATTGCTCGTAAAATAGTTACAAGAATTGTTGAAGGTGAAAAAATTGCTAAATTTACAATAACAAAAGATATTGTTACTGAAATGTTAGGTACTCCTAAATTTACAGATCACCAAGATACGAAAGAACCTCAAGTTGGAGTTGTTAACGGTTTAGCTTACACTTCAATAGGTGGAACAACATTGCAAATCGAAGTTTCAAGCTTCAAGAGTAAAAATAGCGGTATCAAATTAACCGGTTCGCTTAAAGATGTTATGAAGGAATCAGCCAATATTGCTCTTTCATATGTAAGAGCTAATGCTGAAAAATTTGATATTAAAGATTTTGCTTTTGATGAAAGAGAAATTCATATTCACGTTCCAGAAGGAGCTGTTCCTAAAGATGGGCCTAGTGCTGGTGTAACATTTACAACTGCTTTAGTTAGTGCTTTAAGTAATAAACCTGTACCTCAAGATATTGCAATGACAGGTGAAATTACTTTAAGAGGTCGTGTTTTTGAAATTGGTGGATTAAAAGAAAAATCATTTGCTGCATATAAAAAAGGAATCAAAACTGTATTTATTCCAAAAGACAATGAAAAGAATTTAGCAGATATTCCTGAAGAAGTTAAAAAAGCAATTACCTTTATTCCAGTTGAAACTTATGAAGAAATTTATAATCACATTTTTAAAGATCAAAAGCCTACAACTGATAAAAGCGATTCGACTAAAAAGTCTATAAAACAGGCAAATAAGAAATAA", "aa": "MSLFKEDEKNAKLQTEFNNVPILTLVGPPGTGKTSLARAIAEALNKSYIKISLGGVHDESEIRGHRRTYVGSMPGKIIKAIQRAGVSNPLILLDEIDKMSSDYKGDPASAMLEVLDPEQNTKFQDNYLEHEYDLSKVMFIATANYYENIPAPLIDRVEIIELNSYTINEKIKIAREHLIDSTIQQAGLKKEQFNIDDKTLEFIIKHYTAEAGVRGLKRDLDKIARKIVTRIVEGEKIAKFTITKDIVTEMLGTPKFTDHQDTKEPQVGVVNGLAYTSIGGTTLQIEVSSFKSKNSGIKLTGSLKDVMKESANIALSYVRANAEKFDIKDFAFDEREIHIHVPEGAVPKDGPSAGVTFTTALVSALSNKPVPQDIAMTGEITLRGRVFEIGGLKEKSFAAYKKGIKTVFIPKDNEKNLADIPEEVKKAITFIPVETYEEIYNHIFKDQKPTTDKSDSTKKSIKQANKK", "aa_hexdigest": "f6eb224dccc4e6d04b0e4a66fd25e01e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0U7", "query_cov": 0.9271948608137045, "subject_cov": 0.8694779116465864, "identity": 0.674, "score": 573.0, "evalue": 2.45e-200, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A3B0P0U7", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "product": "endopeptidase La" }, "genes": [], "id": "IGIGPGHMMC_791", "locus": "GCA000148625_00764" }, { "type": "cds", "sequence": "contig_1", "start": 438128, "stop": 438694, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C", "UniRef:UniRef90_UPI000FF5D93C" ], "nt": "ATGAAAGAAAAAATCTTACTGTTGGACGTCTTAGATAAAGAATATTTATCTGACGCAGTTTATAATCATGAAACAAAAATTTTAGAAATAAAAACAAAAAACCAAAGTTTTAGTGATCAATATTTAAATCACTTGTTTAATTATTTTGAATCAATTAGTCATGATGAAAAGAAAGAAAAAAATGACCAAGCTAAATATTTATTTATTGGTTATTTTGACTACGCAAAAAACAAATATGATGGCAGCGATGAAAGCAGAAAAAAAATAGTTACAAAAAGTAACAATGTAAACAAATATTGTTCTTATGTTAGAGTGCTTGATTTAAATATTGAATACAAACAAGATTCAAAAGAAATCGATAGATATAAACTTAAAGTAAAAGGAATTGGACGTTATAAAATTACTCATTTAGAAGATAATAGTGAAAAAACACCTGAAAATTCATATAATGTTGAATTTGCAGTTGCTTCAAAAAGTTCTTATGAAAATAAATTAAGTAAAGATTCAGTTAAAAAATTAAATGTATTAGATAAAACTATTACAAACTTAGTTGAAAAAGGATTATGA", "aa": "MKEKILLLDVLDKEYLSDAVYNHETKILEIKTKNQSFSDQYLNHLFNYFESISHDEKKEKNDQAKYLFIGYFDYAKNKYDGSDESRKKIVTKSNNVNKYCSYVRVLDLNIEYKQDSKEIDRYKLKVKGIGRYKITHLEDNSEKTPENSYNVEFAVASKSSYENKLSKDSVKKLNVLDKTITNLVEKGL", "aa_hexdigest": "538bdbb1a9a3e4b5b4c67d5328ad9018", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF5D93C", "query_cov": 1.0, "subject_cov": 0.9082125603864735, "identity": 0.9890000000000001, "score": 361.0, "evalue": 9.85e-126, "valid": true, "uniref50_id": "UniRef50_UPI000FF5D93C" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5D93C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22128.702400000016, "isoelectric_point": 8.606029701232913 }, "id": "IGIGPGHMMC_792", "locus": "GCA000148625_00765" }, { "type": "cds", "sequence": "contig_1", "start": 439102, "stop": 439209, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTTTCAATGCTTATGATAAGATTACTAACTCATTATTAATTAAAGAAATGTACTATGTTTTATTAAACATTTTGGAACCATTTATTCCACATTTAGCTTGA", "aa": "METFNAYDKITNSLLIKEMYYVLLNILEPFIPHLA", "aa_hexdigest": "2aedf62b5f480532fe37bba34bd7b74d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4158.8739000000005, "isoelectric_point": 4.830996131896971 }, "id": "IGIGPGHMMC_793", "locus": "GCA000148625_00766" }, { "type": "cds", "sequence": "contig_1", "start": 439432, "stop": 439668, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "nt": "TTGCACTTACTGTATGCACGTTTCTTTACTAAAGTTTTAGCAGACTTAAAAATGGTTGACTTTAGAGAACCTTTTGATAATTTATTAACTCAAGGAATGATTCTTAAAGATGGACTTAAGATGTCTAAATCAAAAGGCAATGTTGTTAGCCCAGATGAAATGATTAAAAAATATGATGCTGATACTATGAGGCTTTTTGTTCTTTTTGCAGCACCGCCTGAAAAAGAACTTGAATGA", "aa": "MHLLYARFFTKVLADLKMVDFREPFDNLLTQGMILKDGLKMSKSKGNVVSPDEMIKKYDADTMRLFVLFAAPPEKELE", "aa_hexdigest": "24ecdb51c27b920da956198c777c61fb", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002381B5B9", "query_cov": 0.9230769230769231, "subject_cov": 0.8780487804878049, "identity": 0.639, "score": 90.9, "evalue": 1.89e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002381B5B9" }, "pscc": { "uniref50_id": "UniRef50_UPI002381B5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "product": "class I tRNA ligase family protein" }, "genes": [], "id": "IGIGPGHMMC_794", "locus": "GCA000148625_00767" }, { "type": "cds", "sequence": "contig_1", "start": 439867, "stop": 439986, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATTGTGCTAAGTGTGGTTTAGTTCCTGAAAAAGAAGAAAACTTACCAGTTACTTTGCCTTACAAAGTCAACTTTACTACTAGTGGCAACCCACTTAAAACAAATAATAAATGA", "aa": "MINCAKCGLVPEKEENLPVTLPYKVNFTTSGNPLKTNNK", "aa_hexdigest": "9efae394d5bc670e937c1677f55fc07d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4306.978400000002, "isoelectric_point": 8.737867546081542 }, "id": "IGIGPGHMMC_795", "locus": "GCA000148625_00768" }, { "type": "cds", "sequence": "contig_1", "start": 440026, "stop": 440487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGGCAAAATTTGACATAGTTGAAAATAATCAAAATCACTTTAGTAATATTGATATTTTTACAAAAAACAAAGATATTTTAGTAACCATGAATTTTATAACTATTGGTGCAAATCATGGCCTTGTTAAACAATTAATTGAAGAAAATAAATTAAGTGATAAAGAAATTGAAAAACTTAATTTAATTAAAGCAAAAGCTACAGCAAAAGATTTTTCGCAAAAATTATGTTTAGCTTTACCACTTCAAGCAATTAATCCAGTAAATAATCAAAAATACAAAATTTATGTTAGTGATTTTTGTTCTCTCGGAGAAAAAAATAGAAGTCAAATTATCGATACTACTAAAGTTAAAAGTCATGAAGAATTTGCCAAATTTAATAACTTAGAAATTACTAATTATGCAAATAACAAAGCTGATTTTAGTAAGCTTGAAAACTCGAGTAAAATCAACTTACAAGATTGA", "aa": "MAKFDIVENNQNHFSNIDIFTKNKDILVTMNFITIGANHGLVKQLIEENKLSDKEIEKLNLIKAKATAKDFSQKLCLALPLQAINPVNNQKYKIYVSDFCSLGEKNRSQIIDTTKVKSHEEFAKFNNLEITNYANNKADFSKLENSSKINLQD", "aa_hexdigest": "3270587a8835bf9ee5d81e0e62c0dc66", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 128, "aa_cov": 0.8300653594771242, "hmm_cov": 0.9921875, "evalue": 1.6743180832124867e-13, "score": 46.50330352783203, "start": 13, "stop": 139 } ], "seq_stats": { "molecular_weight": 17468.729499999994, "isoelectric_point": 8.439249992370609 }, "id": "IGIGPGHMMC_796", "locus": "GCA000148625_00769" }, { "type": "cds", "sequence": "contig_1", "start": 440944, "stop": 441063, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCAAGTGGAAATATTCATATGGGGCATGTTAGAAACTATGCAATCGGCGATGTTTTAGCACGTTATTATCGTCGTAAAGGTTTTAATGTTTTACATCCTTTTGGTTGA", "aa": "MFPYPSGNIHMGHVRNYAIGDVLARYYRRKGFNVLHPFG", "aa_hexdigest": "d46ec1b438548f1f01d589ed7806e043", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.9487179487179487, "hmm_cov": 1.0, "evalue": 6.25009340741236e-08, "score": 27.757923126220703, "start": 3, "stop": 39 } ], "seq_stats": { "molecular_weight": 4552.2083, "isoelectric_point": 10.15604305267334 }, "id": "IGIGPGHMMC_797", "locus": "GCA000148625_00770" }, { "type": "cds", "sequence": "contig_1", "start": 441785, "stop": 441979, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACAAAATAAAAGTATTTATTATGGCAAAAGTATTGTTAAAAAAATAGTCCAAACTGGAGTTTTATTAGCTTTAGCAATTGTAGTTAATATTACTTGCTCAAGCTTTATGAAATTTCCTTTAGCGCCTTTTTTAAAGTTTGATTTTGCACTTGTTGTCGTTACTTTTAGCGCTTTAGCAATTGCACCTTGA", "aa": "MEQNKSIYYGKSIVKKIVQTGVLLALAIVVNITCSSFMKFPLAPFLKFDFALVVVTFSALAIAP", "aa_hexdigest": "759bf4df9aeac6cfbb6557141f6737f0", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6996.426699999998, "isoelectric_point": 9.581758689880367 }, "id": "IGIGPGHMMC_798", "locus": "GCA000148625_00771" }, { "type": "cds", "sequence": "contig_1", "start": 441966, "stop": 442592, "strand": "-", "frame": 3, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.9", "GO:0004845", "GO:0009116", "RefSeq:WP_013354614.1", "SO:0001217", "UniParc:UPI0001A8C2FF", "UniRef:UniRef100_C4XFB8", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_C4XFB8" ], "nt": "ATGTTAAAAGTTTTAGATCATCCGCTTATTTCAATTAAGCTGACTAATATGAGAGATAAAGAAGCAAATCACTCTCGTTTTAGAAGAAACTTAAATGAAATCGCATCATTAATGGTGTATGAAGTTTTAAGAAACTATAAAACTAAAGCTAGAAAAATAGTGACACCTTTAAATCAAGAATATGTAGGAGCTACTTTTGATAAAGAAATAGTTATTGTGCCAATTTTAAGAGCTGGGCTTGGAATGTTAGATGGCTTACTTGAATTAATGCCTGAAGCTAGAGTTGGTCACATTGGACTTTACCGCGATGAAGTAACTCATCAAGCTCATGAGTATTTTTACAAAATGCCAGAAGTTAAAAAAGATAGCTACATTTTTGTAGTTGATCCAATGCTCGCTACTGGCGGTAGCGCAGTTGATGCTATTAAAAAATTAAAATCAGATGGCTTTACTAATATTCATTTAGTGTGCTTAGTTGGAGTTAAAGAAGGAGTAGCAGCTGTTGAAAAGGCTTTTGGTAAAGACTTTGAAATTTATTTAGCTGCTCTAGACAAAAAACTCAATAATCAAAAATATATTGAACCTGGTTTAGGCGACGCAGGAGACCGTATTTTTGGAACAAAATAA", "aa": "MLKVLDHPLISIKLTNMRDKEANHSRFRRNLNEIASLMVYEVLRNYKTKARKIVTPLNQEYVGATFDKEIVIVPILRAGLGMLDGLLELMPEARVGHIGLYRDEVTHQAHEYFYKMPEVKKDSYIFVVDPMLATGGSAVDAIKKLKSDGFTNIHLVCLVGVKEGVAAVEKAFGKDFEIYLAALDKKLNNQKYIEPGLGDAGDRIFGTK", "aa_hexdigest": "257b399ec7c1330e5d86530cc756a37d", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C2FF", "ncbi_nrp_id": "WP_013354614.1", "uniref100_id": "UniRef100_C4XFB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2FF", "RefSeq:WP_013354614.1", "UniRef:UniRef100_C4XFB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFB8", "uniref90_id": "UniRef90_C4XFB8" }, "psc": { "uniref90_id": "UniRef90_C4XFB8", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "go_ids": [ "GO:0004845", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "IGIGPGHMMC_799", "locus": "GCA000148625_00772" }, { "type": "cds", "sequence": "contig_1", "start": 443066, "stop": 443173, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTCCAGATGCTTTTGTTACAGTTATTTCGCATTCTTTTGAAACAGCTGAAGTTATAAAAGTTTGTGAAATTTTGGAAAACAAGAACATTAATTATGCTTTATGA", "aa": "MGPDAFVTVISHSFETAEVIKVCEILENKNINYAL", "aa_hexdigest": "d3df982869dcd29e230ffbbbb843dc32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3896.4423000000006, "isoelectric_point": 4.571128273010253 }, "id": "IGIGPGHMMC_800", "locus": "GCA000148625_00773" }, { "type": "cds", "sequence": "contig_1", "start": 443173, "stop": 443421, "strand": "-", "frame": 2, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "RefSeq:WP_013354616.1", "SO:0001217", "UniParc:UPI0001E330E7", "UniRef:UniRef100_UPI0001E330E7", "UniRef:UniRef50_UPI0001E330E7", "UniRef:UniRef90_UPI0001E330E7" ], "nt": "ATGCATGAAATAAGTAAAAACATCTTTGCTTACTATACTTCAAGCTTACAAAAAACTTATGAGTCAATTAACAAAGAAGAAATTAAAGAGTATTGCAATTTATTAAGCGAAACTAAAAATCACATATTTTTTGGTATTGGGCAAAGTGAAAAAGTAGCTAGTTATTTACGTGAAAATTTAAACAAAATTAGACTTACTTCATTACCTATTAACAATATGCATGATTTTTTTAGCATAGTTTATGTTTAA", "aa": "MHEISKNIFAYYTSSLQKTYESINKEEIKEYCNLLSETKNHIFFGIGQSEKVASYLRENLNKIRLTSLPINNMHDFFSIVYV", "aa_hexdigest": "7bb5506192e80f83e22f687b38fca44a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330E7", "ncbi_nrp_id": "WP_013354616.1", "uniref100_id": "UniRef100_UPI0001E330E7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330E7", "RefSeq:WP_013354616.1", "UniRef:UniRef100_UPI0001E330E7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330E7", "uniref90_id": "UniRef90_UPI0001E330E7" }, "psc": { "uniref90_id": "UniRef90_UPI0001E330E7", "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_UPI0001E330E7" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E330E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E330E7" ], "product": "RpiR family transcriptional regulator" }, "genes": [ "rpiR" ], "id": "IGIGPGHMMC_801", "locus": "GCA000148625_00774" }, { "type": "cds", "sequence": "contig_1", "start": 443548, "stop": 443721, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354617.1", "SO:0001217", "UniParc:UPI0001E330E8", "UniRef:UniRef100_A0AB32XBI3", "UniRef:UniRef50_C4XFB5", "UniRef:UniRef90_C4XFB5" ], "nt": "TTGTTAAAATTAAGATATGAAAAATTTAAAGTCAAAGTTACTTCAAAAAAATATTTAAAATTTTTACCTGAAAATGAAACGTATAGATTCATAGTCGATTATATCGACAATAAAACAGAAATTTTTTTAAACAACTCTTCAATTGAATTAACTGAAAAAATGTTTGTTTCATAG", "aa": "MLKLRYEKFKVKVTSKKYLKFLPENETYRFIVDYIDNKTEIFLNNSSIELTEKMFVS", "aa_hexdigest": "3a10f14dffe7222b9b1a7448407b93a7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330E8", "ncbi_nrp_id": "WP_013354617.1", "uniref100_id": "UniRef100_A0AB32XBI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330E8", "RefSeq:WP_013354617.1", "UniRef:UniRef100_A0AB32XBI3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBI3", "uniref90_id": "UniRef90_C4XFB5" }, "psc": { "uniref90_id": "UniRef90_C4XFB5", "uniref50_id": "UniRef50_C4XFB5" }, "pscc": { "uniref50_id": "UniRef50_C4XFB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6974.100399999999, "isoelectric_point": 9.260125923156735 }, "id": "IGIGPGHMMC_802", "locus": "GCA000148625_00775" }, { "type": "cds", "sequence": "contig_1", "start": 444255, "stop": 444380, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCTGAATTATGTTCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCACGTTTTGTTATATAG", "aa": "MKAELCSTVLKYENNVLENGIYSNSGLVLSYFSSDFPRFVI", "aa_hexdigest": "beb5d1d27752508245a5a3598068f700", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4649.2559, "isoelectric_point": 4.867770957946777 }, "id": "IGIGPGHMMC_803", "locus": "GCA000148625_00776" }, { "type": "cds", "sequence": "contig_1", "start": 444521, "stop": 444685, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAATGTCAGATTCTAAAATAATGTTTAAAGATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKMSDSKIMFKDDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "18bfa3abdb1bd2c335e94e6ecba71249", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6356.1726, "isoelectric_point": 7.940844917297363 }, "id": "IGIGPGHMMC_804", "locus": "GCA000148625_00777" }, { "type": "cds", "sequence": "contig_1", "start": 445184, "stop": 445402, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTACAAACACTAATTTAATTAAAAATTATTTAGAAAATGATTATTTATTAGCTGTTAAAGATTTAAGAGAATATTTCATAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNTNLIKNYLENDYLLAVKDLREYFINKLIYLLHEKFNYGDVY", "aa_hexdigest": "15caef378a4fc0f00bb59b16fd4d993f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.452699999998, "isoelectric_point": 4.867032051086425 }, "id": "IGIGPGHMMC_805", "locus": "GCA000148625_00778" }, { "type": "cds", "sequence": "contig_1", "start": 445514, "stop": 445681, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "1160043971d8aa939f463149be73f6fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6202.908700000004, "isoelectric_point": 7.942263221740723 }, "id": "IGIGPGHMMC_806", "locus": "GCA000148625_00779" }, { "type": "cds", "sequence": "contig_1", "start": 446411, "stop": 447043, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATTGAAACAACAGTTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSSKSLTSIINGLNEFNSFVESSNQISFEKLDEFINKTQAILNDENNLTTSIQSVIKEFLNAYNSNVDSIETTVRLAKLNNSKQIIDEFVQKSKIELNSLK", "aa_hexdigest": "beb44c1c06e9e97a51f7aebf68fc9063", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 446213, "stop": 447043, "observations": { "mutation": [ 446332, 446413 ], "stop": [ 446332, 446413 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9570000000000001, "score": 369.0, "evalue": 2.45e-127, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9420289855072463, "score": 494.0, "evalue": 3.05e-179, "description": "Internal stop codon at 446332, 446413. Nonsense mutation around 446332, 446413.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23710.505700000023, "isoelectric_point": 5.4200754165649405 }, "id": "IGIGPGHMMC_807", "locus": "GCA000148625_00780" }, { "type": "cds", "sequence": "contig_1", "start": 447098, "stop": 447562, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTADEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "d58bf7ad8ae3df16d904eca596400295", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 447062, "stop": 447823, "observations": { "mutation": [ 447100, 447577, 447592, 447616, 447694 ], "stop": [ 447100, 447577, 447592, 447616, 447694 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.935, "score": 271.0, "evalue": 2.78e-90, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8932806324110671, "score": 432.0, "evalue": 5.35e-156, "description": "Internal stop codon at 447100, 447577, 447592, 447616, 447694. Nonsense mutation around 447100, 447577, 447592, 447616, 447694.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17768.86899999999, "isoelectric_point": 5.962433052062988 }, "id": "IGIGPGHMMC_808", "locus": "GCA000148625_00781" }, { "type": "cds", "sequence": "contig_1", "start": 447692, "stop": 447823, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "IGIGPGHMMC_809", "locus": "GCA000148625_00782" }, { "type": "cds", "sequence": "contig_1", "start": 448074, "stop": 448241, "strand": "-", "frame": 3, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTACTCCCTTGAAATAAGAACGCTCATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATGCAAAAACAAAAAAGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKK", "aa_hexdigest": "d72a3c85770b93ad34e400e9ef47f997", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 65.9, "evalue": 3.47e-13, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "IGIGPGHMMC_810", "locus": "GCA000148625_00783" }, { "type": "cds", "sequence": "contig_1", "start": 448302, "stop": 448394, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "41d0c69f95dae21c1992124a4f6617f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3540.9487, "isoelectric_point": 4.6018781661987305 }, "id": "IGIGPGHMMC_811", "locus": "GCA000148625_00784" }, { "type": "cds", "sequence": "contig_1", "start": 448396, "stop": 448554, "strand": "-", "frame": 2, "gene": null, "product": "transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83", "UniRef:UniRef90_UPI0028527D83" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGTTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATGA", "aa": "MSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVK", "aa_hexdigest": "4d2caef1bccf1cce25b0674586a48bbd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 448411, "stop": 448704, "observations": { "mutation": [ 448557 ], "stop": [ 448557 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0028527D83", "query_cov": 0.9038461538461539, "subject_cov": 0.47474747474747475, "identity": 0.915, "score": 89.4, "evalue": 4.19e-22, "gene_start": 1, "gene_end": 47, "reference_start": 51, "reference_end": 97, "reference_sequence": "MIYIDAVHFHVVDNNVVTKKATYVIMGINGEGQKEILGLYIGENESAKFWMSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIEI" }, "paralog": false, "identity": 0.9072164948453608, "score": 177.0, "evalue": 1.65e-59, "description": "Internal stop codon at 448557. Nonsense mutation around 448557.", "psc": { "uniref90_id": "UniRef90_UPI0028527D83", "product": "transposase", "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0028527D83", "UniRef:UniRef50_UPI0028527D83" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83" ], "product": "transposase" } }, "id": "IGIGPGHMMC_812", "locus": "GCA000148625_00785" }, { "type": "cds", "sequence": "contig_1", "start": 448735, "stop": 449115, "strand": "-", "frame": 2, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFB2", "UniRef:UniRef90_C4XFB2" ], "nt": "ATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATAGGTTACGAAAAAATGATAATAAAGTTCAAAAAAAATTATAGAAATGATTTTTTTAAGAAAAATGTTAATAGTCAATATGGCCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCAAAAATTGAACCTATAATCATCAAGAAGTGTGAACGTGATATTTCTGAATTAGTTGATATGATATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAATGATTTTATGTTTAGCAAATATGGTGTTAATTATTCTCCAGCACAAATAAGTCAATTAACTAACGAAATTGTCGAAGACGCAAGATTATGA", "aa": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARL", "aa_hexdigest": "f68bd9483c5e921774c802e29186cde5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 448432, "stop": 449115, "observations": { "mutation": [ 448557, 448737 ], "stop": [ 448557, 448737 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB2", "query_cov": 1.0, "subject_cov": 0.49606299212598426, "identity": 1.0, "score": 249.0, "evalue": 5.66e-82, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARLWQERKLETYYSIIYIDAVHFHVVDNNVVTKKATYVIMGINSDGQKEILGLYIGENESTKFWMSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVKWYERILQRFKICISI" }, "paralog": false, "identity": 0.9911894273127754, "score": 443.0, "evalue": 6.84e-161, "description": "Internal stop codon at 448557, 448737. Nonsense mutation around 448557, 448737.", "psc": { "uniref90_id": "UniRef90_C4XFB2", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFB2", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB2", "UniRef:UniRef50_C4XFB2", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB2" ], "product": "Mutator family transposase" } }, "id": "IGIGPGHMMC_813", "locus": "GCA000148625_00786" }, { "type": "cds", "sequence": "contig_1", "start": 449570, "stop": 449698, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATGAGTTATACAATGTCATATTAACTTTAAGAAAAGAAATTAAAGAAAACTTTGAAAAAGATGTTCTAGTTATTGAAGAAGAACTTAAAAAAAGCGGTCATAAAAAATTAGCAGAAATATGA", "aa": "MIYELYNVILTLRKEIKENFEKDVLVIEEELKKSGHKKLAEI", "aa_hexdigest": "592dbaac8b237b3688e3fe44e99cd674", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5047.9042, "isoelectric_point": 5.739283180236817 }, "id": "IGIGPGHMMC_814", "locus": "GCA000148625_00787" }, { "type": "cds", "sequence": "contig_1", "start": 449855, "stop": 450277, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTGAATATGACAATAATGTTTTGTATGACAAATCATTAGATATGACATTATCGAAAAATCAAGAAAACATAGCTCAAATATGTGACATGATGGATATGATAATTGGCAGCAGTCAAACCGGAATAATAAATAGTGATAATGTTGAATTAAATACTTTAATGAAAGAAGTTGAATCAGCAGTTAATTTTAAAGACAAATTGAGCTTTTTTACAAGTCAAAGTGGAAAAGACAAATTTTACCAATTCTTCGATAAAATGCTTTATTTAGCTTTAGATGTTAACGCTCAAATATTCAATGATTCAAGATTATTAGATGGTTTAACAGAAGACGATGAAAATCAACAACAAAGAAGTAGATTCTATATTCTTAAGCATAAATATTTTGAAAAAAATAAACAAATTTTAAATGAAGACTGA", "aa": "MSFEYDNNVLYDKSLDMTLSKNQENIAQICDMMDMIIGSSQTGIINSDNVELNTLMKEVESAVNFKDKLSFFTSQSGKDKFYQFFDKMLYLALDVNAQIFNDSRLLDGLTEDDENQQQRSRFYILKHKYFEKNKQILNED", "aa_hexdigest": "d439b38e85e9fa7d10c06e131121e0cb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16448.27789999999, "isoelectric_point": 4.554019737243652 }, "id": "IGIGPGHMMC_815", "locus": "GCA000148625_00788" }, { "type": "cds", "sequence": "contig_1", "start": 451195, "stop": 451833, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "ATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGA", "aa": "MLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTY", "aa_hexdigest": "62ccb3cb0c9a68c7c406150ba77312b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 218, "aa_cov": 0.9952830188679245, "hmm_cov": 1.0, "evalue": 9.688960276081787e-72, "score": 237.5041961669922, "start": 1, "stop": 211 } ], "seq_stats": { "molecular_weight": 24290.117800000015, "isoelectric_point": 4.9036363601684565 }, "id": "IGIGPGHMMC_816", "locus": "GCA000148625_00789" }, { "type": "cds", "sequence": "contig_1", "start": 451840, "stop": 452937, "strand": "+", "frame": 1, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281", "SO:0001217", "UniRef:UniRef50_A0A953T6S3", "UniRef:UniRef90_C4XEU9" ], "nt": "ATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAG", "aa": "MGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK", "aa_hexdigest": "37df700e8a13e5c038b373723d1a13b1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "5-prime", "pseudogene": { "start": 451540, "stop": 452937, "observations": { "mutation": [ 451831 ], "stop": [ 451831 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU9", "query_cov": 1.0, "subject_cov": 0.5496987951807228, "identity": 1.0, "score": 701.0, "evalue": 5.64e-250, "gene_start": 1, "gene_end": 365, "reference_start": 300, "reference_end": 664, "reference_sequence": "MRRAMNALDIKKKIIELSNKLNQWNKEYYQDNNPSVSDFEYDKALQELEQLEKKYPQFIYVNSPTQKLGSFADNKFKKVVHQKPMLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTYWNKMGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK" }, "paralog": false, "identity": 0.9978494623655914, "score": 905.0, "evalue": 0.0, "description": "Internal stop codon at 451831. Nonsense mutation around 451831.", "psc": { "uniref90_id": "UniRef90_C4XEU9", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0A953T6S3", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU9", "EC:6.5.1.2", "UniRef:UniRef50_A0A953T6S3", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0A953T6S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A953T6S3" ], "product": "DNA ligase" } }, "id": "IGIGPGHMMC_817", "locus": "GCA000148625_00790" }, { "type": "cds", "sequence": "contig_1", "start": 453070, "stop": 453564, "strand": "+", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.1", "GO:0004807", "GO:0006096", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P50919", "UniRef:UniRef90_C4XEV0" ], "nt": "ATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGA", "aa": "MNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPI", "aa_hexdigest": "daac67b9ba546fb79c39dce817c0ffaf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 452998, "stop": 453786, "observations": { "mutation": [ 453064, 453562 ], "stop": [ 453064, 453562 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV0", "query_cov": 1.0, "subject_cov": 0.6259541984732825, "identity": 1.0, "score": 319.0, "evalue": 1.02e-108, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MYNLINYLGVIMDIRKYVVIGNWKMNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPIWAIGTGKVATPEIAQDVCQYIHSITSKELVVQYGGSVNPKNISDLAAQKDINGFLVGGASLNVDSFIKLITLGK" }, "paralog": false, "identity": 0.9885496183206107, "score": 509.0, "evalue": 3.66e-186, "description": "Internal stop codon at 453064, 453562. Nonsense mutation around 453064, 453562.", "psc": { "uniref90_id": "UniRef90_C4XEV0", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P50919", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV0", "EC:5.3.1.1", "UniRef:UniRef50_P50919", "KEGG:K01803", "GO:0004807", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P50919", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50919" ], "product": "Triosephosphate isomerase" } }, "id": "IGIGPGHMMC_818", "locus": "GCA000148625_00791" }, { "type": "cds", "sequence": "contig_1", "start": 453877, "stop": 454614, "strand": "-", "frame": 2, "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "db_xrefs": [ "EC:3.5.99.6", "GO:0004342", "GO:0006044", "RefSeq:WP_013354622.1", "SO:0001217", "UniParc:UPI0001A8C230", "UniRef:UniRef100_C4XEV1", "UniRef:UniRef50_C4XEV1", "UniRef:UniRef90_C4XEV1" ], "nt": "ATGAAAGTATATATTGAAAAAGATGCTTTAGCAGTAGCACAAAAAGCATTAAGTTTAATTAAGAATCAAATTACTAAAAAAGCAGATTCAAGAATTTGCTTTGCCACAGGTTCAACACCTTTAGATTTATATAAATTATTAATTGAAGATTATCAAAAAGGTAAAATTGATTTTAGTAAAATGGTTACTTTTAACTTGGATGAATACCTTGGATTAGATTTAAAAAATGAACATTCTTATCATTATTTTATGGATCATAATTTGTTTAATCACATTAATGTTAAAAGAGAAAATATCAATTTTCCTGATGTCATAAAATATGAAAAAGAACATCAAAATGGTAGCTATGAACAAGTAATTGAAAAACTTGGAGGTATTGATTTTACTATTTTAGGTATTGGCGAAAACGGTCATATTGCATTTAATGAACCAGATAGTCAAGAACAAGATATTACAAGAGTAGTTGATTTAACTGAAAGCACAATTAATGCAAACAAAAGATTTTTTGCTAGTGAAAAAGATGTTCCTCGTAAAGCAGTAACTATGGGAATAGGAACTATTTTAAAAAGTAAAAAAATTATGTTATTAGCAACCGGGCCTAAAAAAGCTCAAGCAATTTATGATACTTTACTTAAACCAGTAAATTCAAATTGTCCAGCCAGCTTTTTACAAAATCATAAAGATGTAACAATTATTGTTGATGAATTATCAGCGAAATTAATCAAAAATAAAATTTAA", "aa": "MKVYIEKDALAVAQKALSLIKNQITKKADSRICFATGSTPLDLYKLLIEDYQKGKIDFSKMVTFNLDEYLGLDLKNEHSYHYFMDHNLFNHINVKRENINFPDVIKYEKEHQNGSYEQVIEKLGGIDFTILGIGENGHIAFNEPDSQEQDITRVVDLTESTINANKRFFASEKDVPRKAVTMGIGTILKSKKIMLLATGPKKAQAIYDTLLKPVNSNCPASFLQNHKDVTIIVDELSAKLIKNKI", "aa_hexdigest": "a3a0e82afc87760eaa544d701bc3c27e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C230", "ncbi_nrp_id": "WP_013354622.1", "uniref100_id": "UniRef100_C4XEV1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C230", "RefSeq:WP_013354622.1", "UniRef:UniRef100_C4XEV1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV1", "uniref90_id": "UniRef90_C4XEV1" }, "psc": { "uniref90_id": "UniRef90_C4XEV1", "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "ec_ids": [ "3.5.99.6" ], "uniref50_id": "UniRef50_C4XEV1", "go_ids": [ "GO:0004342", "GO:0006044" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEV1" ], "product": "Glucosamine-6-phosphate deaminase" }, "genes": [ "nagB" ], "id": "IGIGPGHMMC_819", "locus": "GCA000148625_00792" }, { "type": "cds", "sequence": "contig_1", "start": 454627, "stop": 455112, "strand": "-", "frame": 2, "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "db_xrefs": [ "EC:3.5.1.25", "GO:0006044", "GO:0008448", "KEGG:K01443", "SO:0001217", "UniRef:UniRef50_L0RWM1", "UniRef:UniRef90_C4XEV2" ], "nt": "ATGAGTGGAATTGATTCAAGAAAACCGGGAATCTTAGAAGCTTCATTAGAAAGACATGATGTTTATAGTGAATTAATTGTTGATTTTCATCACATTTGTCAAGAAAGTATAGTTTTTACGCTTAATCATAAAGATATAAACCACTTAATGTGTGTTAGCGATGCAATTCGACCTGCTTATGGACCTGATGGTAAAAGCATTTCAGGTGGAATTAATATTGAAAAACATGGTTTAACAATTTATTTAGAGGGCACAACAACTATAGCTGGAAGTGGAATTTGCTTACATGATTCATTTAAAAATTTAGCTTCATTAGATTTAGATTATCGTGATATTGTTAAATTAACTTCATATAATGCAGCTAAAGCTCATAATTTAAAAGATCGAGCACAAATTAAAGCAAATAAATTAGCAGATTTAGTTTTAATGGATTTAAAAACTTTAGATATTAAAGAAGTTTATATTGATGGGCAAAAAGTCAAATAA", "aa": "MSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK", "aa_hexdigest": "c2794937e22f911759187dc2058a9756", "start_type": "ATG", "rbs_motif": null, "genes": [ "nagA" ], "truncated": "3-prime", "pseudogene": { "start": 454627, "stop": 455412, "observations": { "mutation": [ 455121, 455337 ], "stop": [ 455121, 455337 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV2", "query_cov": 1.0, "subject_cov": 0.4410958904109589, "identity": 1.0, "score": 323.0, "evalue": 8.47e-109, "gene_start": 1, "gene_end": 161, "reference_start": 205, "reference_end": 365, "reference_sequence": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTASWENIQIALKNIALAKCQGAKNIGIHIEGPFIGEAKKGAHKLEWLRRATREDILEMLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHLWNAMSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK" }, "paralog": false, "identity": 0.9923371647509579, "score": 517.0, "evalue": 1.25e-187, "description": "Internal stop codon at 455121, 455337. Nonsense mutation around 455121, 455337.", "psc": { "uniref90_id": "UniRef90_C4XEV2", "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "ec_ids": [ "3.5.1.25" ], "uniref50_id": "UniRef50_L0RWM1", "kegg_orthology_id": "K01443", "go_ids": [ "GO:0006044", "GO:0008448" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV2", "EC:3.5.1.25", "UniRef:UniRef50_L0RWM1", "KEGG:K01443", "GO:0006044", "GO:0008448" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWM1" ], "product": "N-acetylglucosamine-6-phosphate deacetylase" } }, "id": "IGIGPGHMMC_820", "locus": "GCA000148625_00793" }, { "type": "cds", "sequence": "contig_1", "start": 455119, "stop": 455301, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTCAAATCATCAATTGAAAAAAATTAGTTATGATCCTTTAATGGTTGAAGAAAAATTAGTTCCTTTTATGGTTCAAAATGACATTATGCCTTCAATTGGTCACTCAGCTGCAAGCTATGATCAAGCTATGAAATACTATGATTTGGGTTCAACTAATACTTGTCACTTATGA", "aa": "MLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHL", "aa_hexdigest": "0a06cc54445ef5fe5a341ea80c828741", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6806.729700000002, "isoelectric_point": 5.756505393981934 }, "id": "IGIGPGHMMC_821", "locus": "GCA000148625_00794" }, { "type": "cds", "sequence": "contig_1", "start": 455461, "stop": 455724, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01979.26" ], "nt": "ATGCTAATAAAAAACGTTAAAATAGTTAATCACAACCAAATTATTGAGTGTGCTGACATTGAGTTTAGTAAAAAAATTACTAAAATAACTCCAACTAAAAATAAGGCTCAATATTTATTAGTTCCAGGCTTTATTGACACTCATATTCATGGTTTTATGAATAAGGATGTAATGGATAGTAGTGCAGCAGTTGAAGATATTAGTTTAAATTTAGCTAGAAATGGAATTACTAGTTTTATGCCTACAGCAATGACAGCAAGTTGA", "aa": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTAS", "aa_hexdigest": "6961c50969b1e300b5bdb9937c776592", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01979.26", "name": "Amidohydro_1", "length": 58, "aa_cov": 0.5287356321839081, "hmm_cov": 1.0, "evalue": 1.3014753545159478e-07, "score": 27.16958236694336, "start": 40, "stop": 85 } ], "seq_stats": { "molecular_weight": 9636.156699999994, "isoelectric_point": 7.90738582611084 }, "id": "IGIGPGHMMC_822", "locus": "GCA000148625_00795" }, { "type": "cds", "sequence": "contig_1", "start": 455957, "stop": 456370, "strand": "+", "frame": 2, "gene": null, "product": "Phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "nt": "ATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGA", "aa": "MKKTIDDLVLKGKKVLMRVDFNVPLKDGVITSNKRIVAALPTIEKVIKEKGKLILFSHLGRVKSEEDKAKNNLLPVANELARLLKKPVLFVDSTRGAELEKAIAKMEPGEVILVQNTRYEDLNEKAESKNNPILGKY", "aa_hexdigest": "34e8d9ace610e7b2b32a6d05df889a28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378MFN7", "query_cov": 0.9343065693430657, "subject_cov": 0.8767123287671232, "identity": 0.617, "score": 151.0, "evalue": 1.64e-44, "valid": false, "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A378MFN7", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_A0A378MFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "product": "Phosphoglycerate kinase" }, "genes": [], "id": "IGIGPGHMMC_823", "locus": "GCA000148625_00796" }, { "type": "cds", "sequence": "contig_1", "start": 456482, "stop": 456901, "strand": "+", "frame": 2, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "nt": "ATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGA", "aa": "MEKEVNALTKAIKNPKKPYVAIIGGAKVSDKIQVLENLIPLVDKMIIGGGMAYTFLKSQGYKIGKSLVEDEFIPFATEFLKKNKDKIILPVDHACAKEFADVEPVVYGLDIDDNYDGLDLGPKSIEKFVSALEGAKTVV", "aa_hexdigest": "c0d9705ec49c9e350b532b7ff52e19a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ACD8294", "query_cov": 0.8992805755395683, "subject_cov": 0.984251968503937, "identity": 0.536, "score": 124.0, "evalue": 4.22e-34, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI002ACD8294", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI002ACD8294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "IGIGPGHMMC_824", "locus": "GCA000148625_00797" }, { "type": "cds", "sequence": "contig_1", "start": 456911, "stop": 457141, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTGCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAG", "aa": "MGVTEFENYKTGTLEVAKAVAALKGCYSIVGGGDSVAAVQKLKMESKFSHVSTGGGASLEFLQGIALPGVEAIQNK", "aa_hexdigest": "df63e89285e91b8aa4874a865f3a43e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002A7EA9FD", "query_cov": 1.0263157894736843, "subject_cov": 0.9176470588235294, "identity": 0.5770000000000001, "score": 85.5, "evalue": 2.57e-20, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A166IH01", "kegg_orthology_id": "K00927" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 65, "aa_cov": 0.8421052631578947, "hmm_cov": 1.0, "evalue": 5.040220069870711e-25, "score": 84.2491226196289, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7790.853999999998, "isoelectric_point": 6.544691658020019 }, "id": "IGIGPGHMMC_825", "locus": "GCA000148625_00798" }, { "type": "cds", "sequence": "contig_1", "start": 457196, "stop": 457321, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCAAGATACAAAATTCATTACAGGAATTATGATTAGCACTAATAAAATTTCATTAGTTATTGGTGATTATGCACGTAAGTTAGCTCTTGAAATGAATGAATATTTAGAAAAGTAA", "aa": "MDKLQDTKFITGIMISTNKISLVIGDYARKLALEMNEYLEK", "aa_hexdigest": "9cc1c20f22f06a4ddd509858ca720f13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4735.540999999999, "isoelectric_point": 6.060423469543457 }, "id": "IGIGPGHMMC_826", "locus": "GCA000148625_00799" }, { "type": "cds", "sequence": "contig_1", "start": 457722, "stop": 458006, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGA", "aa": "MSKKRLLSGIKPTGDLTLGNYIGALRNFVKLQENYDEAYYFVADLHALTTGYVDPKELYQARREIVTMYLACGLDPKKSTIFYQSDVVEHTLAQ", "aa_hexdigest": "12e4e8eaa0cebc5bd1158ac7bc69e37f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10710.146499999997, "isoelectric_point": 6.712594032287597 }, "id": "IGIGPGHMMC_827", "locus": "GCA000148625_00800" }, { "type": "cds", "sequence": "contig_1", "start": 458010, "stop": 458723, "strand": "+", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAA", "aa": "MTTEISIGELSRMTQFKDKSQKMSKQANGTEKIPVGLLMYPVLMAADILIYNPDFVPIGEDQKQHLELARTIAERLNRDYKTNFKLAEGIIPPVGARIMSLTDPTKKMSKSDKGTKATIYLHDDPEVAYKKIMKSVTDSENKVYISEDKPGVLNLLNIYASLTNKTLKEAEKEFKDKDYAQFKSAVAIVVKDELTKIQANYAKATKMVDKVVAEGAQKAQAICQPIVEDLMKKMGYR", "aa_hexdigest": "6bb5c218c02ae2bd8eb195662435a1b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 0.9915611814345991, "subject_cov": 0.9791666666666666, "identity": 0.574, "score": 260.0, "evalue": 1.1e-84, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "IGIGPGHMMC_828", "locus": "GCA000148625_00801" }, { "type": "cds", "sequence": "contig_1", "start": 458987, "stop": 459421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "ATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGA", "aa": "MKIQVDKLLNHTTSHLLGAAVEKLYPEVKLGFGPATEEGFYYDFEFKEPLSVNELSKIEKLMKKLASRNLVTIQIPEKDYDFKNKPYKKELYDELKAQGKKITFYALQDPLNKEIIFKDLCAGGHVESTKVIKNFKLLSIAGAY", "aa_hexdigest": "4a2b8493d6aab52ff09f2d16689b2883", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.2569444444444444, "hmm_cov": 0.8378378378378378, "evalue": 2.8513225539677064e-08, "score": 29.6651611328125, "start": 103, "stop": 139 } ], "seq_stats": { "molecular_weight": 16492.008699999984, "isoelectric_point": 8.765911293029784 }, "id": "IGIGPGHMMC_829", "locus": "GCA000148625_00802" }, { "type": "cds", "sequence": "contig_1", "start": 459617, "stop": 460123, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGA", "aa": "MYIHNEIKNLVLKMDRKYGFTEVLTPHFGSEDLYKTSGHLAHYKDDMFAPLVIENERLIPRPMTCPHHIVCYNLEKRSYRDLPIRYSEQSQLYRYEKSGALTGLERVRGMLLTEGHLFVREDQIEQEIKSMYQLIEETLKIFNIDISYISLSLRDKNDKEKYFNDNKM", "aa_hexdigest": "1733c70d9481a3e87c054679e4699825", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.6130952380952381, "hmm_cov": 0.9809523809523809, "evalue": 1.8397174183886376e-18, "score": 63.21187973022461, "start": 54, "stop": 156 } ], "seq_stats": { "molecular_weight": 20087.83030000001, "isoelectric_point": 6.385428810119628 }, "id": "IGIGPGHMMC_830", "locus": "GCA000148625_00803" }, { "type": "cds", "sequence": "contig_1", "start": 460139, "stop": 460429, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGA", "aa": "MLKKALDDMGVKYTTVVGEAAFYGPKIDIQIDTALGHEITVSTIQLDFLQPKNFDITYTDKNGKEARPVMIHRGLIGTYERFVAILLEQTKGNLPF", "aa_hexdigest": "1c0de043d776b6270796897da626c34f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8645833333333334, "subject_cov": 0.8217821782178217, "identity": 0.639, "score": 109.0, "evalue": 2.29e-29, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 89, "aa_cov": 0.8645833333333334, "hmm_cov": 0.9775280898876404, "evalue": 1.4383198652341005e-15, "score": 53.78683853149414, "start": 7, "stop": 89 } ], "seq_stats": { "molecular_weight": 10774.359399999992, "isoelectric_point": 6.056899452209472 }, "id": "IGIGPGHMMC_831", "locus": "GCA000148625_00804" }, { "type": "cds", "sequence": "contig_1", "start": 460583, "stop": 460735, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAG", "aa": "MSKSKYQVILGANEEKDKTISYREYGKEDTKTVTVEEFINILTKLRNNYE", "aa_hexdigest": "1018cd1ce96b3544e9adc90a81fea955", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 42, "aa_cov": 0.84, "hmm_cov": 1.0, "evalue": 2.166319151158797e-06, "score": 23.8287296295166, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 5918.595899999999, "isoelectric_point": 6.2491857528686525 }, "id": "IGIGPGHMMC_832", "locus": "GCA000148625_00805" }, { "type": "cds", "sequence": "contig_1", "start": 461291, "stop": 461818, "strand": "+", "frame": 2, "gene": null, "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354629.1", "SO:0001217", "UniParc:UPI0001E330EE", "UniRef:UniRef100_C4XEV9", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_C4XEV9" ], "nt": "ATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAA", "aa": "MKKIICVIDMLEGFCNEGALASPIVKKIIPNIEKVLKDNKKEDNLFICDSHNTFDLEMKQYPLHCLKDTKEAEIVKELKPYVKSVLEKNSTNAFHLFDKKLIEKYDQFVLTGCCTDICVLQFALSLKTYLNENRIDKKVIVLKDAVATFDAPGHNAQQFHNFTLNLMANAGIEIQ", "aa_hexdigest": "d5053b3198c14a52349f1c515f70fa1e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330EE", "ncbi_nrp_id": "WP_013354629.1", "uniref100_id": "UniRef100_C4XEV9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EE", "RefSeq:WP_013354629.1", "UniRef:UniRef100_C4XEV9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV9", "uniref90_id": "UniRef90_C4XEV9" }, "psc": { "uniref90_id": "UniRef90_C4XEV9", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_833", "locus": "GCA000148625_00806" }, { "type": "cds", "sequence": "contig_1", "start": 461821, "stop": 462102, "strand": "+", "frame": 1, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354630.1", "SO:0001217", "UniParc:UPI0001E330EF", "UniRef:UniRef100_C4XEW0", "UniRef:UniRef50_C4XEW0", "UniRef:UniRef90_C4XEW0" ], "nt": "ATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAA", "aa": "MTKTIDLHKLNVEQATSKVILALSEAQEKKLTSLEIITGYGSGALRARTLELLESENLTYIEEGPMVIVYFFNNNEDENDDNFSDIEYQKKFQ", "aa_hexdigest": "e7b8c658c22a427049059c3588eff924", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330EF", "ncbi_nrp_id": "WP_013354630.1", "uniref100_id": "UniRef100_C4XEW0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EF", "RefSeq:WP_013354630.1", "UniRef:UniRef100_C4XEW0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEW0", "uniref90_id": "UniRef90_C4XEW0" }, "psc": { "uniref90_id": "UniRef90_C4XEW0", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEW0" }, "pscc": { "uniref50_id": "UniRef50_C4XEW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW0" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_834", "locus": "GCA000148625_00807" }, { "type": "cds", "sequence": "contig_1", "start": 462157, "stop": 462342, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGACTTTTATTTAATATACTTAGCTTTACAATTATTAGTTCCTTTTGGACTTGAAATATCAGTCAAAGAAGAAATTGTCAACAAGCCTTTAGAAAGTGATTTAACTTTTGAAGGTGAAAAAGAACAAAATAAAGATAATAATCAGGAAAATAATCAAACACAATCAAAAAATAGTAAATAG", "aa": "MDDFYLIYLALQLLVPFGLEISVKEEIVNKPLESDLTFEGEKEQNKDNNQENNQTQSKNSK", "aa_hexdigest": "6cba0285e07590f901d81f9ad8ff4fde", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7090.793200000002, "isoelectric_point": 4.339623069763184 }, "id": "IGIGPGHMMC_835", "locus": "GCA000148625_00808" }, { "type": "cds", "sequence": "contig_1", "start": 462850, "stop": 463200, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTTGGTATTTCATTAACTTTAACTATCCCTGGCTTAATTGTTCTTTTAATAGGACAAAAAGCTAAAAAATATGTGCTGAAAGATGTTATTACCAAATTTGATAAATATAAATTAATCAAAAAATTATTTAACTTCTTTACTCTTGGTTACAAAGAAAATATCGTTGATGAAAATAACAAAATTCACAAAAATTATGACTTAATCAATTTTTATAAAAACACACATAATTTCAAAAATTTTGAATTTGGTTTTGCTCCAATCCATAAAAAATATGAACAATATGAAGCTTTAACTGATAACCAATATTTAAAGATGCAAAATGTTTACTACGAAGGCAACATTTGA", "aa": "MLFGISLTLTIPGLIVLLIGQKAKKYVLKDVITKFDKYKLIKKLFNFFTLGYKENIVDENNKIHKNYDLINFYKNTHNFKNFEFGFAPIHKKYEQYEALTDNQYLKMQNVYYEGNI", "aa_hexdigest": "7fff1fc8bc39a78472e457ec01c4bc53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13861.003299999984, "isoelectric_point": 9.357150840759278 }, "id": "IGIGPGHMMC_836", "locus": "GCA000148625_00809" }, { "type": "cds", "sequence": "contig_1", "start": 463339, "stop": 463470, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTATCAAATAAGTTAATTTCAAAAGAAGCTTTTATTCGTTCTAAACGTCCAACAATAAACAAATTAACTACTCAATTAATTAATAATTATTATGCTGATAAAAAGAAAAGCAAAAGGATTAATTGA", "aa": "MSVSNKLISKEAFIRSKRPTINKLTTQLINNYYADKKKSKRIN", "aa_hexdigest": "69bbbeab83aa6042f17205e2270e75ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5041.871600000003, "isoelectric_point": 10.573346900939939 }, "id": "IGIGPGHMMC_837", "locus": "GCA000148625_00810" }, { "type": "cds", "sequence": "contig_1", "start": 463597, "stop": 464058, "strand": "-", "frame": 2, "gene": null, "product": "Deoxyribose-phosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "nt": "ATGCTTACAAGTGCTAAGGCATTTGAAGCTAAAGAAGCTATTAAAGCTGGTGCTGATGAAATTGACATGGTTATTCAAATTGGAAAAATGAAAATGGGTGACTATGACTATGTTTTAGAAGACATTAAAGCGGTTAAAAAAGCATGCGGCAAACATGTTTTAAAAGTTATTATTGAAACAGCTTTATTAACAGAAGCTGAAATTAGAAAAGCTACTGAAATCGTTATGGCTTCTGATGCTGAATTCATTAAAACTTCAACAGGTTTCTCAACAAGAGGCGCAACACTTGAAGATGTTAAAATCATGAAAAGTGTTTGTGGAGACAAATTATTAATCAAAGCTGCTGGTGGAATCAGCAACAAAGATGACATGATCAACATGGCTCAAGCTGGCGCAAACCGTTTCGGAACAAGCCGTTCAGTTGCCATTGTTGAAGGTAAATCTTCAAAAGAAGGGTACTAA", "aa": "MLTSAKAFEAKEAIKAGADEIDMVIQIGKMKMGDYDYVLEDIKAVKKACGKHVLKVIIETALLTEAEIRKATEIVMASDAEFIKTSTGFSTRGATLEDVKIMKSVCGDKLLIKAAGGISNKDDMINMAQAGANRFGTSRSVAIVEGKSSKEGY", "aa_hexdigest": "ea58885d93b8b8d4fe29a208fefe0b40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00220D32D4", "query_cov": 0.9673202614379085, "subject_cov": 0.9367088607594937, "identity": 0.77, "score": 212.0, "evalue": 2.92e-68, "valid": false, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_A0A139MP28", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0009264" ] }, "pscc": { "uniref50_id": "UniRef50_A0A139MP28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [], "id": "IGIGPGHMMC_838", "locus": "GCA000148625_00811" }, { "type": "cds", "sequence": "contig_1", "start": 464134, "stop": 464265, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTAATTACAACAAAATGATTGATCACACTTATTTAAAAGCTGATGCGACAAATAAAGAAATTGATAATTTATTAGATGAAGCCAAGAAATATGACTTCAAAACTGTTTGTGTTAATTCATCATGA", "aa": "MQINYNKMIDHTYLKADATNKEIDNLLDEAKKYDFKTVCVNSS", "aa_hexdigest": "cec1bd5b04f178dbf5da8c6d6cd17892", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.631500000001, "isoelectric_point": 5.553703880310058 }, "id": "IGIGPGHMMC_839", "locus": "GCA000148625_00812" }, { "type": "cds", "sequence": "contig_1", "start": 464288, "stop": 464512, "strand": "-", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4", "UniRef:UniRef90_A0A3P8MDY4" ], "nt": "ATGAAACTTGGCGCCGGCCGTGCTAAAAAAGAAGATAGTATTGACTTTGAAGCAGGAATTACTCTTCACGCTAAAACAAATCATAAAGTAACTAAAGGGCAAAAACTATTTACTCTTTATTCTTCAAATCCGATTGACAAGAATTTAGTTAAAGAATTAGAAACAGGTTACAAAATTGGCAATTCAAAAGTTGAAAATAAGATTATAATTGCTAAGTTGAAATAA", "aa": "MKLGAGRAKKEDSIDFEAGITLHAKTNHKVTKGQKLFTLYSSNPIDKNLVKELETGYKIGNSKVENKIIIAKLK", "aa_hexdigest": "96115f3718663ff416c9a1c04620841f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.905, "score": 132.0, "evalue": 3.06e-39, "valid": true, "product": "Pyrimidine-nucleoside phosphorylase", "uniref50_id": "UniRef50_A0A3P8MDY4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "IGIGPGHMMC_840", "locus": "GCA000148625_00813" }, { "type": "cds", "sequence": "contig_1", "start": 464810, "stop": 465583, "strand": "-", "frame": 1, "gene": null, "product": "thymidine phosphorylase", "db_xrefs": [ "EC:2.4.2.4", "GO:0006206", "SO:0001217", "UniRef:UniRef50_R5VCS9", "UniRef:UniRef90_A0A3P8LBE3" ], "nt": "ATGCGTGTCGTTGATTTAATCGAAAAGAAAAGAGAAGGTAAAGAATTAACAACTCAAGAAATTGAATTCTTAATTACTTCTTATGTATCAGGTAATACACCTGATTACCAAATGGCTGCTTTTATAATGGCAGTTATGTTCCAAGGAATGGCAAAAAAAGAATTAGCAACTTTTACTAAAACCATGATGCACTCAGGTGAAGTTATTGATTTAAGTGAAATTCCAGGGATTAAAGTTGATAAACACTCAACAGGTGGAGTTGGAGATAAAACTACTTTAGCAGTTGCTCCTATTTGTGCCGCTTTAGGTGCTCCAGTAGCGAAAATGAGTGGTAGAGGTTTAGGCCACACTGGTGGAACAATTGATAAACTTGAATCTATTCCAGGATTTCATGTTGAATTAACTGAACGTCAATTTATTGAACAAGTTAAAAAACATAATATTTCAGTTATTGGTCAATCAAAAGAATTAGTACCAGCTGATAAAAAACTTTATGCTTTAAGAGATGTTACTGCTTGTGTTAACTCAATTCCTCTTATTGCTTCAAGTATTATGTCTAAAAAATTAGCTACAGGTTCAAATGCTATTTTATTAGATGTTAAATGCGGTAAAGGCGCCTTCATGAAAAATGAAGACCAAGCTAAAGAATTAGCTAAAACTATGATTTCAATTGGTAAAGAATTAAACGTTGATGTTCGTGCAGAAATTACCAACATGAACCGTCCAATTGGTTGTGAAATTGGAAACAAAAATGAAGTTTTAGAAGCTATTTGA", "aa": "MRVVDLIEKKREGKELTTQEIEFLITSYVSGNTPDYQMAAFIMAVMFQGMAKKELATFTKTMMHSGEVIDLSEIPGIKVDKHSTGGVGDKTTLAVAPICAALGAPVAKMSGRGLGHTGGTIDKLESIPGFHVELTERQFIEQVKKHNISVIGQSKELVPADKKLYALRDVTACVNSIPLIASSIMSKKLATGSNAILLDVKCGKGAFMKNEDQAKELAKTMISIGKELNVDVRAEITNMNRPIGCEIGNKNEVLEAI", "aa_hexdigest": "56c498535f478982cacbcac4e28cd371", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LBE3", "query_cov": 1.0, "subject_cov": 0.9178571428571428, "identity": 0.9179999999999999, "score": 458.0, "evalue": 8.64e-162, "valid": true, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_R5VCS9", "go_ids": [ "GO:0006206" ] }, "genes": [], "id": "IGIGPGHMMC_841", "locus": "GCA000148625_00814" }, { "type": "cds", "sequence": "contig_1", "start": 465597, "stop": 466301, "strand": "-", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_013354634.1", "SO:0001217", "UniParc:UPI0001E330F2", "UniRef:UniRef100_UPI0001E330F2", "UniRef:UniRef50_S5MIP4", "UniRef:UniRef90_C4XEW4" ], "nt": "ATGACACCACACATTAATGCCAAAGAAGGTGATATTGCTAAGACAGTTTTAATGCCAGGAGATCCTTTAAGAGCTAAATTTATTGCTGAAACATTTTTAGACAAAGGATATAGACTTGTTAATACTGTCAGAAACATGTACATGTATACAGGTACATATAAAGGTCGTCCAGTAACAATCGCTGGAAGTGGAATGGGTTGTCCATCAATTGGGATCTATTCATATGAATTATTCAAATTCTACAATGTAGATAACATTATTAGAATCGGTACAACTGGTTCATACAAAAAAGAAATCAAACTATATGAAACGATTTTAGCAACTGAAGCTTATGCAGATGGTGCTAGTTATCGTCGTTTAGTTTTAGGAGACAAAAGCCATGTAGCTTTACCTAGTGCTAAATTAAATAAAGAAATTGAAAAAATGGCTAAAAATTTGAACATTAAATTAACTTTAGGCCGTGTACACTCTTCAGATGTATTCTATTCATCAGTTCCTCTTCAACAAAGAATCGATGAAACACATGCGCTTTGTGTTGAAATGGAATCATATGCTTTATTTACTAATGCTGAAAAATTAGGTAAAAATGCAGCATGTCTTTTAACAGTTAGTGACAACTTAATTACAAATGACGAAACAACACCCGAAGAAAGACAAACAGCTTTCAAAAACATGATGGAAATTGCTTTAGGTGTTGCAAAATAA", "aa": "MTPHINAKEGDIAKTVLMPGDPLRAKFIAETFLDKGYRLVNTVRNMYMYTGTYKGRPVTIAGSGMGCPSIGIYSYELFKFYNVDNIIRIGTTGSYKKEIKLYETILATEAYADGASYRRLVLGDKSHVALPSAKLNKEIEKMAKNLNIKLTLGRVHSSDVFYSSVPLQQRIDETHALCVEMESYALFTNAEKLGKNAACLLTVSDNLITNDETTPEERQTAFKNMMEIALGVAK", "aa_hexdigest": "927f50ea38f588c2afc6d7baa58b19b7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330F2", "ncbi_nrp_id": "WP_013354634.1", "uniref100_id": "UniRef100_UPI0001E330F2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330F2", "RefSeq:WP_013354634.1", "UniRef:UniRef100_UPI0001E330F2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330F2", "uniref90_id": "UniRef90_C4XEW4" }, "psc": { "uniref90_id": "UniRef90_C4XEW4", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_S5MIP4", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_S5MIP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5MIP4" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "IGIGPGHMMC_842", "locus": "GCA000148625_00815" }, { "type": "cds", "sequence": "contig_1", "start": 466691, "stop": 467428, "strand": "+", "frame": 2, "gene": null, "product": "ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "nt": "ATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGA", "aa": "MKKIKRIAILTSGGDAPGMNSAIRAAAKMARSRGIETFLVYEGYKGLVDDNIVPSTKIDLDFFNSRGGTCIYSARYPQFKDPEIREIAIKNLNKRKIDALIVIGGDGSYAGAQLLHEKGIKTIGLPGTIDNDIASSDFTIGYDTALNTVVRCIDQIRDTAASHKRIMIIEVMGNNCGDLALHTGLATGAEVIATSEYRLDEKEIVKTCVELTTNAKPVRRNITIVVSEKLYNIKQLAKDIEKATG", "aa_hexdigest": "ce9d337683932a4658b53dc08bd059e9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A975M207", "query_cov": 0.9795918367346939, "subject_cov": 0.8791208791208791, "identity": 0.5, "score": 233.0, "evalue": 1.75e-73, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A7V6J2C4" }, "pscc": { "uniref50_id": "UniRef50_A0A7V6J2C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "product": "ATP-dependent 6-phosphofructokinase" }, "genes": [], "id": "IGIGPGHMMC_843", "locus": "GCA000148625_00816" }, { "type": "cds", "sequence": "contig_1", "start": 467552, "stop": 467683, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCCCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAGATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAA", "aa": "MAIGIIDNDIVASPIPEALSMINKSKAKVRDKAIKFNELNRLD", "aa_hexdigest": "efaf9dd0a796476c69e01b9cbefb1b40", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4753.543099999999, "isoelectric_point": 8.140632438659669 }, "id": "IGIGPGHMMC_844", "locus": "GCA000148625_00817" }, { "type": "cds", "sequence": "contig_1", "start": 467910, "stop": 468236, "strand": "+", "frame": 3, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "nt": "ATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTAGATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGA", "aa": "MSQKEDKKPIVFSDVDGTLYARDCYVSNFNLNIIKQDNLSFNIATGNPICPRMLKLAKLVDADYMIGSSGVQIYDYKHNKYLHEEYLKASVVKKIFKLFKESKVSAAA", "aa_hexdigest": "f023992a6533a1e1c792cddc4a92b4ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00210774DB", "query_cov": 0.9074074074074074, "subject_cov": 0.875, "identity": 0.7240000000000001, "score": 147.0, "evalue": 8.7e-44, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI00210774DB" }, "pscc": { "uniref50_id": "UniRef50_UPI00210774DB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "product": "HAD family hydrolase" }, "genes": [], "id": "IGIGPGHMMC_845", "locus": "GCA000148625_00818" }, { "type": "cds", "sequence": "contig_1", "start": 468549, "stop": 468734, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAG", "aa": "MVIGDSENDYSMFHKFNYSYVMDNAKDEVKDKANFITKAVQEHGLGYAVMDYLEKFNKRNN", "aa_hexdigest": "d61069b349d6d13fc53c2b8168a08c7c", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 48, "aa_cov": 0.7868852459016393, "hmm_cov": 1.0, "evalue": 1.0734244227955177e-15, "score": 54.09297561645508, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 7174.945400000002, "isoelectric_point": 5.50738010406494 }, "id": "IGIGPGHMMC_846", "locus": "GCA000148625_00819" }, { "type": "cds", "sequence": "contig_1", "start": 469050, "stop": 469889, "strand": "+", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEW7", "UniRef:UniRef90_C4XEW7" ], "nt": "TTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAA", "aa": "MLKLGVLSQNIIQINLELEENKKLRKSLNLLNFIKEQIINENKYYVLIDEIQNVKNFEDSVNYLLNIKNVDLYITGSNSKFLSRDIETQISSRATEIKVRPLTFKEFYSVKKELNIDEVFKEYLTFGGMPFLVNLNYESKKKYLKELFETTYINDIVERNKIKSDEYLQEFVDFLTSNVGSLTNNLNICNTYKTLKQNNIAYFTIDKYLKNLSHTYLIDLVQLFNLKGKRIIKRLNKIYFYDLGIRNSRIGYNNIKIEHAIENIVYNELVIRNYDVNIG", "aa_hexdigest": "089c8ef35cfe98923aeaf5bea060da5b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEW7", "query_cov": 1.0, "subject_cov": 0.8353293413173652, "identity": 0.996, "score": 538.0, "evalue": 3.05e-192, "valid": true, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XEW7", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_C4XEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW7" ] }, "genes": [], "id": "IGIGPGHMMC_847", "locus": "GCA000148625_00820" }, { "type": "cds", "sequence": "contig_1", "start": 470204, "stop": 471169, "strand": "+", "frame": 2, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "nt": "ATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGA", "aa": "MARFIDEIKITLQAGKGGDGMISFRREAHVDKGGPDGGDGGRGGNIYFIGDLGKNTLLNLYGNKKITAEDGINGGPKNLYGAAGKDTYVKVPLGTVVYKNNKVVADIIEPNKNYLVAQGGQGGRGNMKFKSPRNTAPRICENGTKGEKFEAHIVLKVMSDVGVVGKPSAGKSTLLSVISNAKAKIAEYEFTTLVPQLGLVRYFDNSFTVADLPGLIKGASLGKGLGFQFLKHIERCRVIAHIIDFGLSEKNPIEDYETINNELASYSMHLENKPQIVVANKSDMPDFKDHLKAFKKKYPKVKVIVISALEQENLDQLKAEL", "aa_hexdigest": "e41655d052ef5ddce95ed7a8d8e7ce4d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q3XWL6", "query_cov": 1.02803738317757, "subject_cov": 0.9041095890410958, "identity": 0.515, "score": 313.0, "evalue": 3.57e-102, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q3XWL6", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q3XWL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "product": "GTPase Obg" }, "genes": [], "id": "IGIGPGHMMC_848", "locus": "GCA000148625_00821" }, { "type": "cds", "sequence": "contig_1", "start": 471224, "stop": 471388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09269.16" ], "nt": "ATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGA", "aa": "MVEIKLEDDFTIANPYRGFFEVSGPKVEKIYEKIPLISYDNLIRFNNMLKKIGV", "aa_hexdigest": "ab4751304e8aae3c9754b8556ed596ba", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09269.16", "name": "DUF1967", "length": 46, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.3869444494388253e-10, "score": 37.1574821472168, "start": 10, "stop": 54 } ], "seq_stats": { "molecular_weight": 6337.3634, "isoelectric_point": 6.147955513000488 }, "id": "IGIGPGHMMC_849", "locus": "GCA000148625_00822" }, { "type": "cds", "sequence": "contig_1", "start": 471568, "stop": 472035, "strand": "+", "frame": 1, "gene": null, "product": "Glucosamine-6-phosphate deaminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0", "UniRef:UniRef90_C4XEX0" ], "nt": "ATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGA", "aa": "MAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNF", "aa_hexdigest": "fde3a6b565bab662ecd6ded12682939a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 471550, "stop": 472305, "observations": { "mutation": [ 472033 ], "stop": [ 472033 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX0", "query_cov": 1.0, "subject_cov": 0.6175298804780877, "identity": 1.0, "score": 314.0, "evalue": 4.61e-107, "gene_start": 1, "gene_end": 155, "reference_start": 7, "reference_end": 161, "reference_sequence": "MILRSPMAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNFWLNNDLLNNFKIMQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN" }, "paralog": false, "identity": 0.9920318725099602, "score": 493.0, "evalue": 3.02e-180, "description": "Internal stop codon at 472033. Nonsense mutation around 472033.", "psc": { "uniref90_id": "UniRef90_C4XEX0", "product": "Glucosamine-6-phosphate deaminase", "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX0", "UniRef:UniRef50_C4XEX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0" ], "product": "Glucosamine-6-phosphate deaminase" } }, "id": "IGIGPGHMMC_850", "locus": "GCA000148625_00823" }, { "type": "cds", "sequence": "contig_1", "start": 472069, "stop": 472305, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAA", "aa": "MQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN", "aa_hexdigest": "8465db29de169fbeba7073fa9a33727a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8450.764899999995, "isoelectric_point": 9.901844215393066 }, "id": "IGIGPGHMMC_851", "locus": "GCA000148625_00824" }, { "type": "cds", "sequence": "contig_1", "start": 472339, "stop": 472689, "strand": "-", "frame": 2, "gene": null, "product": "Hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "nt": "ATGACTACTTCTAGTTATGCAGGAGCAGGCAATAGTAGCGGTAGTGTTAAAATCATTATGGACTTGGCTCAAGATATTAGAAATAAAGATGTTTTAATTGTTGAAGACATTATTGATTCAGGAATTACACTTGATAAAATCAAGCAAATTCTTTTATCTCGTAAACCTCGTTCATTTAAAATAATAACTCTTTTAGACAAACCACACAACCGTAAAGTTGAATTGAAAGCTGATAAATCAGCCTTTATCGTGCCAAATGAATTCTTAGTTGGTTTTGGTTTGGATTATGATGAAAAAATGAGAAACCTCCCTTATATTGGAATTTTTGATAAAAAATATTTAAAAAAATAA", "aa": "MTTSSYAGAGNSSGSVKIIMDLAQDIRNKDVLIVEDIIDSGITLDKIKQILLSRKPRSFKIITLLDKPHNRKVELKADKSAFIVPNEFLVGFGLDYDEKMRNLPYIGIFDKKYLKK", "aa_hexdigest": "81af85f6aba0c8dde6570696b4e959be", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PE13", "query_cov": 0.9827586206896551, "subject_cov": 0.8085106382978723, "identity": 0.632, "score": 151.0, "evalue": 6.2e-45, "valid": false, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_A0A3B0PE13", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PE13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "product": "Hypoxanthine phosphoribosyltransferase" }, "genes": [], "id": "IGIGPGHMMC_852", "locus": "GCA000148625_00825" }, { "type": "cds", "sequence": "contig_1", "start": 472807, "stop": 472896, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAGCAAAATATTGACTATCGAATTAAAAAGGTTTTATATACTCAAGAAGAAATTGAAGCTAAAATCAAAGAATTAGCAACTTGA", "aa": "MNKQNIDYRIKKVLYTQEEIEAKIKELAT", "aa_hexdigest": "53592a0486315c4d4995180bac52042d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3511.052100000001, "isoelectric_point": 8.064688682556149 }, "id": "IGIGPGHMMC_853", "locus": "GCA000148625_00826" }, { "type": "cds", "sequence": "contig_1", "start": 472889, "stop": 473422, "strand": "-", "frame": 1, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX2" ], "nt": "ATGTATGAAAAACAACCTAATATTCACATTGGTTTTCAATCATATATTATTGAAAGCTTATCAGACACATTTAAAAAAACAGTAGCTAAATTTAAAAATAATAACTTAAATTTTAGAGACAATCATATCTTTTTAGATCGTTTCAACATCGAACACTATGTTTTTGCTGTTGTCACTTTAATGGATAAAGATCCTAAATATCAAGCAGGTTTTGATGCTATGTTTGAAAAAATAGTTGATCCTGAAGATAATCCGGATTTGGCGATCTTTATTGATATTGATTTTAATGCATTCAAAGCTCGTTTATTAGCTCGAGGTCGCGCTTCAGAAGTTGATAATTATGCTCAAAATGAAACATACTTTAAATTACTTCATTCTTTATATAAAAAGCTTTATATAAGATTAGTTGAAAAATTTAAGATACCTTATGTTATCATTAATAGTAATAACAAAAGTGAAAAAGAAGTTTTACAAGAAGCTATTAATATAATAGATAGCTATGACTTCTCAAAATCAAAGAGGTATAATGAATAA", "aa": "MYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE", "aa_hexdigest": "f606b2530d97c1f8c64984f741358fc3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 472889, "stop": 473572, "observations": { "mutation": [ 473425 ], "stop": [ 473425 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX2", "query_cov": 1.0, "subject_cov": 0.7797356828193832, "identity": 1.0, "score": 350.0, "evalue": 2.36e-121, "gene_start": 1, "gene_end": 177, "reference_start": 51, "reference_end": 227, "reference_sequence": "MGLTMIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLKWMYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE" }, "paralog": false, "identity": 0.9911894273127754, "score": 451.0, "evalue": 2.3e-163, "description": "Internal stop codon at 473425. Nonsense mutation around 473425.", "psc": { "uniref90_id": "UniRef90_C4XEX2", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX2", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "IGIGPGHMMC_854", "locus": "GCA000148625_00827" }, { "type": "cds", "sequence": "contig_1", "start": 473423, "stop": 473560, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTGGTATAAGTGGCATGATTGGCAGTGGTAAAAGTACACTTGCTAAAGGCTTACATAAACATTATAAAAAATCAGTTTTATTAGAAGAATTTCAAGAAAATGACCCAGTTTTTAATACATTTTTAAAATGA", "aa": "MIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLK", "aa_hexdigest": "4738f28a360d814ddd5b12c268723004", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5007.825100000001, "isoelectric_point": 9.225441932678223 }, "id": "IGIGPGHMMC_855", "locus": "GCA000148625_00828" }, { "type": "cds", "sequence": "contig_1", "start": 473761, "stop": 474054, "strand": "-", "frame": 2, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX3" ], "nt": "GTGGAAAACCACACATCTAAATTAGCAGATTGTTTTAAAAAATTTAGCGAAAAAGGCTATAAATACAAGGATAATCACATATTTTTAGATCGTTTTAGTATTGAACATTACATTTTTGCTAATGTTAACTTAAGACCTAAAGGTAAAAAATATTTAGAAGGTTATGATGCACTTTTCTCTCATCTTATTACTAAAGATGAAACTCCAGATTTAGCAATTTTTCTAGATATGACTTTTGACACTTTCAAGAAAAGATTATTTGAAAGAGGTAGAGAAGTTGAAACTGAAAACTGA", "aa": "MENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRPKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETEN", "aa_hexdigest": "7baebc4d7e0039080bf186700ffa63c2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 473569, "stop": 474261, "observations": { "mutation": [ 473763, 474105 ], "stop": [ 473763, 474105 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX3", "query_cov": 1.0, "subject_cov": 0.4217391304347826, "identity": 0.9790000000000001, "score": 200.0, "evalue": 1.59e-63, "gene_start": 1, "gene_end": 97, "reference_start": 70, "reference_end": 166, "reference_sequence": "MKKEVIIMVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLKWLYEKQPNLTIGFQSYVVENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRTKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETENWNKNKDYFRELYDLYKPLFIKQAKKYDLNYVIIETDNLTEKQVFEKAVEVINNYDFSKMERYNK" }, "paralog": false, "identity": 0.9826086956521739, "score": 456.0, "evalue": 1.24e-166, "description": "Internal stop codon at 473763, 474105. Nonsense mutation around 473763, 474105.", "psc": { "uniref90_id": "UniRef90_C4XEX3", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX3", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "IGIGPGHMMC_856", "locus": "GCA000148625_00829" }, { "type": "cds", "sequence": "contig_1", "start": 474103, "stop": 474240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAGGTATAAGCGGAATGATTAGTAGTGGTAAAAGCACACTAACAAGAAATTTAGTTAAACACTACAAAAATTCAATGATGTTAAAAGAATATGAAGAGGACGATGATGTCTTCAATACATTCTTAAAATGA", "aa": "MVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLK", "aa_hexdigest": "ff5e553325210b21fc920c2e7d9c832b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5171.940100000001, "isoelectric_point": 6.530084037780762 }, "id": "IGIGPGHMMC_857", "locus": "GCA000148625_00830" }, { "type": "cds", "sequence": "contig_1", "start": 474349, "stop": 474840, "strand": "-", "frame": 2, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_013354643.1", "SO:0001217", "UniParc:UPI0001A8C247", "UniRef:UniRef100_C4XEX4", "UniRef:UniRef50_A5IYH4", "UniRef:UniRef90_A0A3P8MDY7" ], "nt": "ATGGCAGGTAAAAAGCAAGAAAAAATCATATTAGCTAAAGAAACATTAGAAAAATATCAAGCAGAATATGAACACTTAATTAAAGTTGAAAGACCTGAAATTCAAGCAGCTTTAAAAGAAGCTAGAGCACAAGGTGACCTTTCAGAAAACGCTGAATATGATGCAGCCAGAGATCGTCAAGGTATTGTTGAAGCAAGAATTGTTGAACTTGAAGGCATTTTAGCAAATGCTGAAGTTATTGAAACAAACCAAAACAAAAAAGGTAAACTAAAAGCAGGTATCGGAGCAACAGTTAAATATCTTAACTTAAAAACAAATAAAGAAATTACAGTTAAAATCATGGGTATTCATGATTCTAATCCAATGGAAGGTAATATTTCTAATGAATCACCAGTAGCACAAGCTATCATGGAAGGTGATGAAGGCGCAACAGTTGAAGTTGATGTGCCTAATAAATACAGTATAAAAATCTTATCAATTCAATACAAATAG", "aa": "MAGKKQEKIILAKETLEKYQAEYEHLIKVERPEIQAALKEARAQGDLSENAEYDAARDRQGIVEARIVELEGILANAEVIETNQNKKGKLKAGIGATVKYLNLKTNKEITVKIMGIHDSNPMEGNISNESPVAQAIMEGDEGATVEVDVPNKYSIKILSIQYK", "aa_hexdigest": "9912c554e9a6fd621826c079a4171577", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C247", "ncbi_nrp_id": "WP_013354643.1", "uniref100_id": "UniRef100_C4XEX4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C247", "RefSeq:WP_013354643.1", "UniRef:UniRef100_C4XEX4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEX4", "uniref90_id": "UniRef90_A0A3P8MDY7" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY7", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_A5IYH4", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYH4" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "IGIGPGHMMC_858", "locus": "GCA000148625_00831" }, { "type": "cds", "sequence": "contig_1", "start": 475358, "stop": 475591, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTTCAATTAATTCAACTGAAACAAATCAAGAATTATTTGACAAATATTATCCAATGTTAAGTGAAAAAGGGATGCTTGTTGTATTACAAAATTCAAATACAAAAAGTGGTTATAGAGTTTTAACTAAACATTTAAAACTTAATAAAATTGTTTATGAATTAAGTTATGTAAAAAGCGGATTCTTATATATAGTAAAAAGTAAAACTGAAATAAAAAATGAAAACTAA", "aa": "MIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN", "aa_hexdigest": "dab3e62cf1061dcdea90814a94a25db2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9001.359299999998, "isoelectric_point": 9.363597679138184 }, "id": "IGIGPGHMMC_859", "locus": "GCA000148625_00832" }, { "type": "cds", "sequence": "contig_1", "start": 475685, "stop": 476086, "strand": "-", "frame": 1, "gene": null, "product": "Methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5", "UniRef:UniRef90_C4XEX5" ], "nt": "ATGATTAGTGCTGTAATCAATTTAAGTAAAGGTGCAAAAATTGGCTTAATTGTTTCTGCTATTGTTTTTGTGATTATTGCCTTTATAACTTATATTATTTTACTAGTTAAAACTAGAAAAATTCGTAATGAAATAAATACCAAAGAAGCTCAAAAAGCTCATGAAGCTATTTTGAAAATCAGAGGTGAAGAATTAGGAGAATTCCCTAAAGAATTAAAAGAATTTTTTAAAAGTAATCTTGATGATTATGATTTAGAAAACTTTATTAATACTATTTATTTAAATGATGTTCAAGAGATTTTACTTGTTGGAAGTAAGTTAGAATATCCAACAGCTTTATTTAAAAATAAATCACAAGCTCAAATTTGTTTAGAAAAACAAAACATGAATGTAAGTTTATGA", "aa": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQNMNVSL", "aa_hexdigest": "572804c1c177a7f3bcdaabb328ea76d9", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 475382, "stop": 476086, "observations": { "mutation": [ 475687 ], "stop": [ 475687 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX5", "query_cov": 1.0, "subject_cov": 0.5495867768595041, "identity": 0.992, "score": 242.0, "evalue": 2.79e-79, "gene_start": 1, "gene_end": 133, "reference_start": 1, "reference_end": 133, "reference_sequence": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQKMNVSLWNKAVLELPKYFKDQPSFINVNELKTQDKKFKLIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN" }, "paralog": false, "identity": 0.9871794871794872, "score": 445.0, "evalue": 1.43e-161, "description": "Internal stop codon at 475687. Nonsense mutation around 475687.", "psc": { "uniref90_id": "UniRef90_C4XEX5", "product": "Methyltransferase", "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX5", "UniRef:UniRef50_C4XEX5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5" ], "product": "Methyltransferase" } }, "id": "IGIGPGHMMC_860", "locus": "GCA000148625_00833" }, { "type": "cds", "sequence": "contig_1", "start": 476079, "stop": 476513, "strand": "-", "frame": 3, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "EC:3.1.21.10", "GO:0006364", "RefSeq:WP_013354645.1", "SO:0001217", "UniParc:UPI0001E330FB", "UniRef:UniRef100_A0AB32XC51", "UniRef:UniRef50_A5IYK4", "UniRef:UniRef90_A0AB32XC51" ], "nt": "ATGCGAGCTTTAGCTCTAGATTTAGGCACTAAAACTTGCGGTTTTGCAATTAGTGATGAAGGCATGATTATAGCTAGTGCACTTGAAACATTACATTTTGAAGAAAATGATTTTAATGCAATTATTGAGCAAATCAAAATTTACCAAAAAACATATAAAATAGATAAATTGGTATTAGGAAAGCCTTTAAGATCTAATAATACAAGTAGTGAAAGAACAGTGCTTTTTGAATCTTTTGCTCATAAACTAAAAGGTATTTTTAAAGACTACAAAATTTATCAAGTTAATGAATATGGAACTACTATAGCTGCTACTAATATTTTAAAAGAAGCAAAACTTTCTATTAAAAAAAGAAAAGCTCATAAAGATACTTTGTCAGCTGTTTTAATCTTGCAAGAATTTTTAAATTATGGAGGTGTAGAATTACATGATTAG", "aa": "MRALALDLGTKTCGFAISDEGMIIASALETLHFEENDFNAIIEQIKIYQKTYKIDKLVLGKPLRSNNTSSERTVLFESFAHKLKGIFKDYKIYQVNEYGTTIAATNILKEAKLSIKKRKAHKDTLSAVLILQEFLNYGGVELHD", "aa_hexdigest": "e8fd5b429388892961df9e96ae9ae147", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E330FB", "ncbi_nrp_id": "WP_013354645.1", "uniref100_id": "UniRef100_A0AB32XC51", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330FB", "RefSeq:WP_013354645.1", "UniRef:UniRef100_A0AB32XC51" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC51", "uniref90_id": "UniRef90_A0AB32XC51" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC51", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A5IYK4", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYK4" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "IGIGPGHMMC_861", "locus": "GCA000148625_00834" }, { "type": "cds", "sequence": "contig_1", "start": 476513, "stop": 477238, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02272.25", "PFAM:PF07973.19" ], "nt": "ATGGGCGCAATTATGACTATTGACGAAAGTGAATACATGGATCCTAATAACATTCGTATTGTTGACTTTAAAGGTATTACAAAAGACCTTTGCGGTGGTACTCACTTAGATAGAACCGGAATTTTAGAAGACTTTAAAATTACTAAAGTTGAGAAAAAAACTGCTGGTGTATTTAGAATAAGAGCTGTAGCTTCTCATAAATATGTTAAACAATATTTAACAGAATTAAATCAAAGTTTAAAAGAACAATTAGATGCTTTAGTTAAAAAAGCTCAAAGCTTTGATAGTAAATATCAAATTAAATTTACACCTAATAAAGATGTTGAAATTGAATGTGCAAACATTAAAAAAACAATTGATAAAGTTCATGAAGATATCAAAGAATTAATCAAAAACAAAAGCAATATAACCTTTGATTTTGATAGTGTTCAATTAACTAAAAACAAAAAATACCCTTATTATTTAAACATTGATTTTGATAGTTCCCAAGTTAAAGTTGCTGCTGCAACGCTTAGAGAAAAATTTCCTGAAGCTATAATTGTTTTAGGTGCTAAAAGTAATAATCAAATTCTTTTATGTGTGGCCGCTAAAGTGCTTGATGCTAACAAATTATTCCAACAAATAGCTTCAAAAACCAATGGTCGTGGTGGTGGAAGTTCAATTATTTCAATGGGTAAAGTTGACTATACTAAAGATCTTGAAAATATTATTAAGGAACTTATTTAA", "aa": "MGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI", "aa_hexdigest": "7d29e705574c7529a619c84fc12dbbe1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02272.25", "name": "DHHA1", "length": 89, "aa_cov": 0.35269709543568467, "hmm_cov": 0.9775280898876404, "evalue": 2.1881095074553326e-09, "score": 33.768638610839844, "start": 156, "stop": 240 }, { "id": "PF07973.19", "name": "tRNA_SAD", "length": 43, "aa_cov": 0.17842323651452283, "hmm_cov": 1.0, "evalue": 9.989052060467751e-14, "score": 47.12787628173828, "start": 18, "stop": 60 } ], "seq_stats": { "molecular_weight": 27148.053900000024, "isoelectric_point": 8.986135292053223 }, "id": "IGIGPGHMMC_862", "locus": "GCA000148625_00835" }, { "type": "cds", "sequence": "contig_1", "start": 477296, "stop": 478429, "strand": "-", "frame": 1, "gene": "alaS", "product": "alanine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.7", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419", "SO:0001217", "UniRef:UniRef50_Q98Q12", "UniRef:UniRef90_C4XEX7" ], "nt": "ATGCAAGATGCTCCAACTAACTATGACAGCGATCTTTTTATTAACATAATTAAAGAAATAGAAAAATATGCCACAGTTAAATATGACATTGATAACTACTTTAAAAAAGATAAAAAGCAAACTGAAATCAACACTAACTTTAAAGTTATAGCAGACCACATTAGAACAGTGGTTAATGCTATAGCTGATGGAGCTAAAGTTTCAAATGTTGGCCGTGGTTACATTATTAGAAGACTTATTCGACGTTCAATTTACAAAGGAATGCAACTAGGAGTTAAAGGTCTATTTTTACATAAATTAGTAGATGTAGTACAAGATTCATTACCTTATGAATATGACAAAAAACCTGTTGTAGTAGCCATTAAAGAAGAAGAATTAGCTTTCCATCAAACTATTGAAAAAGGTAAATTATTACTTGAAAAATTTATGGATAATAAAACTAAAATTTTCTCAGGTGAAGATGCTTTTAATTTACTTGAAACTTATGGCTTTCCAATTGAGTTAACTGTTGAAATTTTAGCTAAGAAAAATATTAAAGTTGATCTTAAAGCTTTTGAAGAAGCTAAGGAAAGACACGCCAACTTAAGCAGGGGTGAAAAAGTTAACGGAATGGATAAAGTAATTAACTCATTAGCCTTAATTAAAGGCAAAATCGGTGAATTTATTGGTTATACAGACTTAGAAAGTAAATCAAAAATTCTTAAACTTTTAAATAATGAGGAAGAGGTAAAATCATTAAATGGTGAAGGTTATTTGATCTTAGATAAAACACCTTTTTATGCTACTAGTGGTGGTCAAAATCATGACCATGGTTTCATTTTACAAGGTAAAAATAAAATCAAAATTTTAGATGTTTTTAAAGACAAATTTGGTAACCATATTCATAAAATTGAAGGCAAAATTAACAATAAGGATACTGTCGAATGTTATGTTGATAAAGAAGTAAGAATTGGTATGGCTCGAGGTCACTCAGCAACTCACTTATTATTCCGTGCTTTAGTTAAAGTACTTGGACCTCATATTGATCAACTTGGTTCAGATATCACCGAAGAACGTTTAATATTTGACTTTCCAGCTGATGAAAAACCAACTGATGAACAAATTAAAAAAATCGAAGACATAATGCATGGTTGA", "aa": "MQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHG", "aa_hexdigest": "ac67aa2f7429c6039832759aba239c3a", "start_type": "ATG", "rbs_motif": null, "genes": [ "alaS" ], "truncated": "both", "pseudogene": { "start": 476993, "stop": 478729, "observations": { "mutation": [ 477298, 478537, 478663, 478723 ], "stop": [ 477298, 478537, 478663, 478723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX7", "query_cov": 1.0, "subject_cov": 0.4308571428571429, "identity": 1.0, "score": 744.0, "evalue": 1.2e-263, "gene_start": 1, "gene_end": 377, "reference_start": 238, "reference_end": 614, "reference_sequence": "MTMEKLTSKEIREKWLRYFESKGHLRVESKSLIPVNDPSLLWINSGVATLKDYFSGKKKPPRNRLTNSQKAIRTNDIENVGVTSRHHTFFEMLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKYWMEQGVEESHLIPGTRDTNFWDVGSGPCGPDTEIFYDRGPKYNKRGLELLKKDIENDRYIEIWNIVFSQFNNDGENNYTELKQKNIDTGAGLERIASIMQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHGWIKQNVEREYIVTNIKKAKEMGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI" }, "paralog": false, "identity": 0.9930795847750865, "score": 1137.0, "evalue": 0.0, "description": "Internal stop codon at 477298, 478537, 478663, 478723. Nonsense mutation around 477298, 478537, 478663, 478723.", "psc": { "uniref90_id": "UniRef90_C4XEX7", "gene": "alaS", "product": "alanine--tRNA ligase", "ec_ids": [ "6.1.1.7" ], "uniref50_id": "UniRef50_Q98Q12", "go_ids": [ "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX7", "EC:6.1.1.7", "UniRef:UniRef50_Q98Q12", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q12" ], "product": "Alanine--tRNA ligase" } }, "id": "IGIGPGHMMC_863", "locus": "GCA000148625_00836" }, { "type": "cds", "sequence": "contig_1", "start": 478721, "stop": 478867, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTAACTTTTCAATTGGCGATTACTTTAAAAAAGAAGCTATCGCTTTTGGTTATGAATTCTTAACTAATGAATTACAAATAGACCCTAAAAAACTTTACATGACTTATTTTGCAGAAGATTTGGATACTAAAAAATACTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKY", "aa_hexdigest": "ad41bcfa2a8b765b721ac15ac798e365", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5711.468700000001, "isoelectric_point": 4.7658586502075195 }, "id": "IGIGPGHMMC_864", "locus": "GCA000148625_00837" }, { "type": "cds", "sequence": "contig_1", "start": 479144, "stop": 479863, "strand": "-", "frame": 1, "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "db_xrefs": [ "EC:2.8.1.13", "GO:0008033", "GO:0016740", "GO:0016783", "KEGG:K00566", "SO:0001217", "UniRef:UniRef50_Q600M2", "UniRef:UniRef90_C4XEX8" ], "nt": "ATGGGTCATTATGCCAAAGTTAAGAATGGTAAACTTTATCGGGCGAAAGATCAAAATAAAGATCAAACATATTTTTTAGCTCAATTAACTAATCAACAACTAGAAAAAGTTTTAATGCCTCTTGCTGATTATCCTAAAGAAGAAATAAGAAAAATAGCTAAAAAATTAGACTTAGCTACAGCTACAAAAAAAGATTCAACAGGTATTTGTTTTATTGGCGAAAGACATTTTACAAAATTCTTACAAAACTATATTCCAGCTCAAAAAGGAAAAATATTAGACATTAAAACTAAAGAAGTTTTAGGAACTCATGATGGTTGTTTCTATTACACTATCGGTCAAAGAAAAGGATTAAATTTAGGCGGCAAAAGTGAAGCTCACTATGTTTGCGGTCATGATGTCAAAAACAATATTATTTATGCTGCACCAGCTAGTGATCCTAGTTATTTAGAAAGTAATATGCTAATAGCTTCAAACTTATGTCTAAATCATCATCGCTTTAATAAAAACAAATTAAGTGCTAAATTTAGATATCGTCAAAAAGATCTTAAAGTTAAAGTTGAATTCTTAGAAAATAATAAAATTAAAGTATTTTATCCATCAAAAGCTCAAGCAGTAACCCCCGGTCAACAAGTTGTTTTATATGATCAAGACAAGTGCTTAGGCGGAGCAATAATTGAAGAAATATACTTAAACAATATTAAAAAAGATTACATTTAG", "aa": "MGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI", "aa_hexdigest": "e6786ae432ac4a22986b42ab2e220612", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmA" ], "truncated": "3-prime", "pseudogene": { "start": 479144, "stop": 480163, "observations": { "mutation": [ 480004, 480151 ], "stop": [ 480004, 480151 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX8", "query_cov": 1.0, "subject_cov": 0.6256544502617801, "identity": 1.0, "score": 477.0, "evalue": 3.9e-168, "gene_start": 1, "gene_end": 239, "reference_start": 144, "reference_end": 382, "reference_sequence": "MKLIFNIRINMHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRNWDSYVNNDFLGNENISQDICPQEQDYQDALAVANKLGIKLHRVDFVKEYWDNVFENFIEEYKKGRTPNPDILCNKYIKFDAFLKYAFEKLNADYIAMGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI" }, "paralog": false, "identity": 0.9941002949852508, "score": 687.0, "evalue": 1.81e-253, "description": "Internal stop codon at 480004, 480151. Nonsense mutation around 480004, 480151.", "psc": { "uniref90_id": "UniRef90_C4XEX8", "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "ec_ids": [ "2.8.1.13" ], "uniref50_id": "UniRef50_Q600M2", "kegg_orthology_id": "K00566", "go_ids": [ "GO:0008033", "GO:0016740", "GO:0016783" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX8", "EC:2.8.1.13", "UniRef:UniRef50_Q600M2", "KEGG:K00566", "GO:0008033", "GO:0016740", "GO:0016783" ] }, "pscc": { "uniref50_id": "UniRef50_Q600M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q600M2" ], "product": "tRNA-specific 2-thiouridylase MnmA" } }, "id": "IGIGPGHMMC_865", "locus": "GCA000148625_00838" }, { "type": "cds", "sequence": "contig_1", "start": 480149, "stop": 480262, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCATAAAAAGAAAGTTATTTTAGGAATGTCTGGAGGAGTAGACTCTTCAGTAGCTGCATATTTATTACTTCAACAAGGTTATGATGTTGAAGGTCTTTTTATGCGGAATTGA", "aa": "MHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRN", "aa_hexdigest": "bb6bd5eed5ff4427b49b46d59b44511e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 22, "aa_cov": 0.5945945945945946, "hmm_cov": 1.0, "evalue": 1.5676657903409157e-07, "score": 26.732755661010742, "start": 2, "stop": 23 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.918918918918919, "hmm_cov": 1.0, "evalue": 6.710272797980482e-13, "score": 44.75435256958008, "start": 4, "stop": 37 } ], "seq_stats": { "molecular_weight": 4056.7289999999994, "isoelectric_point": 8.160811042785646 }, "id": "IGIGPGHMMC_866", "locus": "GCA000148625_00839" }, { "type": "cds", "sequence": "contig_1", "start": 480954, "stop": 481043, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTTTAACTATTGCTCGTGCTGTTAATATTGTTTTAGAAAACGGATTAAGACTTTTAGGTGTTAGTTCAAAACACAAAATGTAA", "aa": "MNSLTIARAVNIVLENGLRLLGVSSKHKM", "aa_hexdigest": "10bc86e11d2336a6882e93b0147c64d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3165.7748, "isoelectric_point": 11.000127601623536 }, "id": "IGIGPGHMMC_867", "locus": "GCA000148625_00840" }, { "type": "cds", "sequence": "contig_1", "start": 481083, "stop": 481268, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "TTGCTTAAGAAATCTAAATTAGATGTTTCAAAACTTAAAGCAACAAATATTGACAACGAACTTGATAAAAAATTAGTTTCGACATTGCTTGAATTTCCAGAAGTTATCATTAGTTCAGCAACTAAGTACTTAACACACTTACTAACACAATACTTAATTAGAGTTGCCAAAGAATTTAACTCATGA", "aa": "MLKKSKLDVSKLKATNIDNELDKKLVSTLLEFPEVIISSATKYLTHLLTQYLIRVAKEFNS", "aa_hexdigest": "67f166eb88701eb9e028287d6f393c8a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 62, "aa_cov": 0.9836065573770492, "hmm_cov": 1.0, "evalue": 2.8744510735207937e-10, "score": 36.31890106201172, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6981.156399999999, "isoelectric_point": 9.34754505157471 }, "id": "IGIGPGHMMC_868", "locus": "GCA000148625_00841" }, { "type": "cds", "sequence": "contig_1", "start": 481431, "stop": 481559, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGCAATGCAAGACTTAGGTTACGACCCAAACAAATTAATTGTTCTTTGTTTACAATTAGTAAGACTTATGAAAGATGGTCAAGAATACAAAATGTCAAAACGTAAAGGTACAAGTTTTTGA", "aa": "MKIAMQDLGYDPNKLIVLCLQLVRLMKDGQEYKMSKRKGTSF", "aa_hexdigest": "c6255dd01225134f784e6a40e94c48ce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8623, "isoelectric_point": 9.579889106750485 }, "id": "IGIGPGHMMC_869", "locus": "GCA000148625_00842" }, { "type": "cds", "sequence": "contig_1", "start": 481989, "stop": 482582, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGGATAAAAACATTATTAAAAGCAAAATTATTAAAGAATTAAAACCAATTTTAAACAAATTAGGGATTGAAAAGGACGATCTCTTAATTGAACCTAAAGTCAAAGCCGATTTCGCTTCTAACGTTGCATTAGGAAACAAAGGCAAAAATCCTATGGAACTTGCCAAAAAAATTGTTAAAGAATTAGAGCCTAAACTAACTTCATTAGGTCTAAAAAGTGTAGAAATTGCAGCTCCTGGATTTTTAAACTTTTACATTGATGATCAAACAATTATTAAAAGTTTGAACCAAATTATCAAACAAAATGAAAACTTTGGTCGTGGTAAATTAAAAGGAAATCTAAACATCGAATGGGTTTCAGCAAACCCTACTGGATACTTGCACATTGGTCATGCACGTAATGCTGCTATTGGATCAGCACTATACAACATTTGTACTTTCGCAGGTTTAAATGTTTTACGTGAATATTACATCAATGATGGTGGTAACCAAATTAACACTTTAGCTCAAAGTATATTTGCAAGATATAAACAATTTTATGAACCAGATTTCCCTATGCCAGAAGACTGCTATGGAGGTCAAGACATTGTTTGA", "aa": "MDKNIIKSKIIKELKPILNKLGIEKDDLLIEPKVKADFASNVALGNKGKNPMELAKKIVKELEPKLTSLGLKSVEIAAPGFLNFYIDDQTIIKSLNQIIKQNENFGRGKLKGNLNIEWVSANPTGYLHIGHARNAAIGSALYNICTFAGLNVLREYYINDGGNQINTLAQSIFARYKQFYEPDFPMPEDCYGGQDIV", "aa_hexdigest": "34b945c66ddadab51a07dedeb599802f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 87, "aa_cov": 0.41116751269035534, "hmm_cov": 0.9540229885057471, "evalue": 2.4847676367686356e-13, "score": 46.527488708496094, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 21959.130199999992, "isoelectric_point": 8.692804145812985 }, "id": "IGIGPGHMMC_870", "locus": "GCA000148625_00843" }, { "type": "cds", "sequence": "contig_1", "start": 482854, "stop": 483189, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0", "UniRef:UniRef90_C4XEY0" ], "nt": "ATGTTTAAGAAAAAACTATTAATATCAAGTTTAGCAGTTGCTTCATTTTTACCTGTATCAGTTGTTTGTGTCTCTTGTGGAGATGGTAAAACTGATGAAGAAAAATACTTAGAAGCTGCTAAAGTTAAATTTGAAAACATTAAAGCATATTTAGCCAAAATAAATGACTCTTATGAAGAGTATCAAAAAACTACAAAAATAACTGATTCTGCAATTATTGCTAAAAGAGATAAAGCTAAAAAACTTGGCGAAAAAATGAAATCTGAAAATTACTACAGCGAATTAATTAAAGACAAAAAAATAATAGAAATAAATACTATTTTAAATGACTTATGA", "aa": "MFKKKLLISSLAVASFLPVSVVCVSCGDGKTDEEKYLEAAKVKFENIKAYLAKINDSYEEYQKTTKITDSAIIAKRDKAKKLGEKMKSENYYSELIKDKKIIEINTILNDL", "aa_hexdigest": "72eec781810d4f72e02ba356db2a85c2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEY0", "query_cov": 1.0, "subject_cov": 0.8740157480314961, "identity": 1.0, "score": 208.0, "evalue": 9.2e-68, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEY0" }, "pscc": { "uniref50_id": "UniRef50_C4XEY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0" ], "product": "Lipoprotein" }, "genes": [], "id": "IGIGPGHMMC_871", "locus": "GCA000148625_00844" }, { "type": "cds", "sequence": "contig_1", "start": 483206, "stop": 483538, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTACTTGCAGGCGTTAACAAAGATTATGTTGACTATTCAGTAGACAGTAGTCTAGGAAATGCAACACTTGAAGAATATGTAAAAGCTATTGATGCTTCAAGAGCTGAAATGTACAAAAACTTAATTATTTCTAGAGTTGCATTAGTATGTGTATTAGGAATATTTGCAATGTTAACATTCCTTCCTGCAGTTATTGAAGATGCAATTAACAAGAAAAAATATGGTTCTTTAGAAACTTATGAAGAATACAAAGCACAAAAATTAGCAGAATTGTCAAAAGAAGAAGCTACTTTAACTAAACAAAAACCAGTTGAAGTTCAAGCTTAA", "aa": "MFLLAGVNKDYVDYSVDSSLGNATLEEYVKAIDASRAEMYKNLIISRVALVCVLGIFAMLTFLPAVIEDAINKKKYGSLETYEEYKAQKLAELSKEEATLTKQKPVEVQA", "aa_hexdigest": "25fdb73ff790f550619a8d5400bec70e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12260.048999999988, "isoelectric_point": 4.990429496765136 }, "id": "IGIGPGHMMC_872", "locus": "GCA000148625_00845" }, { "type": "cds", "sequence": "contig_1", "start": 483974, "stop": 484366, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCCAGATCAAACATTTGCAGAAGCAATTAAATCAGGTAAAAGTGTTACTGCTTTACCTAAATTAGGAGCTGGTCTTGGTGTATTCCTTGCTGTTGCTGCTATCTTCTTTGCTTATGATGGATTCTATGTCGCTGCTGGAATTCAAAGTGAAATGAAAGAACCTAAAAAGACTCCTTTAGCAATTTTCATTGGTTTAGGAATCACAACCTTAATTTACTTAATTGTTGCTATTTCAATGTCACTTAACGGTGGATCATTCTTTGGCTTCAATGGATACATGAGTAGTTTATTCAAAAATGAAAAAGCTGGTCGAATTGTTTTTGGTTTAATTAACATTTGTATTTCAATTGGTGTTCTAGGTATTATTAATGGTTTCTCAATGTGA", "aa": "MPAPDQTFAEAIKSGKSVTALPKLGAGLGVFLAVAAIFFAYDGFYVAAGIQSEMKEPKKTPLAIFIGLGITTLIYLIVAISMSLNGGSFFGFNGYMSSLFKNEKAGRIVFGLINICISIGVLGIINGFSM", "aa_hexdigest": "0a0cda4308aec5277add6979894ad13c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.043899999986, "isoelectric_point": 9.13157596588135 }, "id": "IGIGPGHMMC_873", "locus": "GCA000148625_00846" }, { "type": "cds", "sequence": "contig_1", "start": 484559, "stop": 484684, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTATATCTATTTACCTCTTACATACTTATTTATGCCTATGTATGTAATCATGTCACTTCAAGATGGTGTTGGGGCATTAATGAATAAAGATGCTTTAACATTCGGAACAAATGCTGACTGA", "aa": "MTYIYLPLTYLFMPMYVIMSLQDGVGALMNKDALTFGTNAD", "aa_hexdigest": "8845ad847763da78011822c1c28973e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4624.438300000001, "isoelectric_point": 4.16927661895752 }, "id": "IGIGPGHMMC_874", "locus": "GCA000148625_00847" }, { "type": "cds", "sequence": "contig_1", "start": 484823, "stop": 484975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAAAACAAACAAAAGAAAAGAAGATTTCCTTTATAGCTGCTATGTTGATCGTTATAGGTGGATCAATTGGTGCTGGTATCTTCTTTAAATCAGGTGCTGTTTTAAAAAGCTCGCACAGTAGTTTGATTCTAGCCATTTTCTGTTGA", "aa": "MEEKQTKEKKISFIAAMLIVIGGSIGAGIFFKSGAVLKSSHSSLILAIFC", "aa_hexdigest": "9af944d4ff5ad9f2f66088e98547419b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.333899999998, "isoelectric_point": 9.430902671813964 }, "id": "IGIGPGHMMC_875", "locus": "GCA000148625_00848" }, { "type": "cds", "sequence": "contig_1", "start": 485029, "stop": 485961, "strand": "-", "frame": 2, "gene": null, "product": "Carbamate kinase", "db_xrefs": [ "RefSeq:WP_013354651.1", "SO:0001217", "UniParc:UPI0001E33100", "UniRef:UniRef100_C4XEY2", "UniRef:UniRef50_P78030", "UniRef:UniRef90_C4XEY2" ], "nt": "ATGTCAAAAATTGTTATAGCTTTAGGTGGAAACGCTTTAGGAAACAATCCTGAAGAACAAAAAGAATTAGTTAAATTACCTGCTAAAAAAATAGCAGATTTAGTTAAAGAAGGTCACCAAGTGCTTGTTGGTCATGGTAATGGTCCTCAAGTTGGAATGATCTTCAATGCCTTTGCTGATGCAAAAAAAGTTAATGAAAAAACTCCATTAGTACCTTTTGCAGAAGCAGGTGGTATGTCTCAAGGCTACATTGGTTATCACATGCTCACAGCGATAACCAATGAATTGAGAAAGAAAAAAATTGAAAAAGATGTTTTATACTTTTTGACTCAAACAATAGTTGATGCTAAAGATAAAGCATTTAAAAACCCTACAAAGCCAGTTGGTCCTTTCTATAAAACTTTAGAAGAAGCTGAATTGGCTAATCCTAATTCAACCATTAAAGAAGATGCTGGTCGTGGTTTTAGAAAAGTTGTTCCTTCACCACTTCCTCTTGACTTTATTGGAATGAAAACAATTAGAAAAACATTTGACGCTGATAATGTTGTTATAGTTGGTGGTGGTGGTGGCATACCTACTATCGACGAAAAAGGACAATATAAAGGTGTTGATGGCGTTATCGACAAAGATTTCGCTTTAAGTAAATTGGCTACAAAAGTTGAAGCAAACTTATTTGTGATTCTTACAGCAGTAGAACATGTTTGTGTTAACTTTGGTAAACCAAACCAAAAAGCTTTAAAAGAAGTAACTGTTTCTGAACTTGAACAATACATCAAAGAAGAACAATTTGCTCCAGGAAGTATGCTTCCTAAAGTTAAAGCTGCTATTGCTTTTGTTAACGAAAATCCAAAAAATGTAGCTGTTATTGCTGACTTAAACAAAGTATCACAAGCTATCGAAGGCAAAAGTGGTACAAGAATTATCAAAGGATAA", "aa": "MSKIVIALGGNALGNNPEEQKELVKLPAKKIADLVKEGHQVLVGHGNGPQVGMIFNAFADAKKVNEKTPLVPFAEAGGMSQGYIGYHMLTAITNELRKKKIEKDVLYFLTQTIVDAKDKAFKNPTKPVGPFYKTLEEAELANPNSTIKEDAGRGFRKVVPSPLPLDFIGMKTIRKTFDADNVVIVGGGGGIPTIDEKGQYKGVDGVIDKDFALSKLATKVEANLFVILTAVEHVCVNFGKPNQKALKEVTVSELEQYIKEEQFAPGSMLPKVKAAIAFVNENPKNVAVIADLNKVSQAIEGKSGTRIIKG", "aa_hexdigest": "9389ce12ac167fa28f76f2a1b13e5d56", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33100", "ncbi_nrp_id": "WP_013354651.1", "uniref100_id": "UniRef100_C4XEY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33100", "RefSeq:WP_013354651.1", "UniRef:UniRef100_C4XEY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEY2", "uniref90_id": "UniRef90_C4XEY2" }, "psc": { "uniref90_id": "UniRef90_C4XEY2", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [], "id": "IGIGPGHMMC_876", "locus": "GCA000148625_00849" }, { "type": "cds", "sequence": "contig_1", "start": 486018, "stop": 486266, "strand": "-", "frame": 3, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGAAAATGATTAAAGCTGCAAAAGAAGACGTTATCTTCTTACACTGCTTACCAGCTTTCCACGATGACCACACATTATTCTCAGCTGAAATCAAAGAAAAATTTGGTAAAAAATACCCAGTAGTTGCAACTGGAGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATGCAACAGATCAATTGATCAAGCTGAAAACCGTTTACACACAATTAAAGCTGTTGTTTTAGCAACTATTGGTTACTAA", "aa": "MKMIKAAKEDVIFLHCLPAFHDDHTLFSAEIKEKFGKKYPVVATGAMEVTDEVFQSKCNRSIDQAENRLHTIKAVVLATIGY", "aa_hexdigest": "3b36ec5ac8edc23a46c8307d9ab284c0", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 80.5, "evalue": 3.27e-18, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "IGIGPGHMMC_877", "locus": "GCA000148625_00850" }, { "type": "cds", "sequence": "contig_1", "start": 486336, "stop": 486641, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGTTGGCAGACTATATGACAATTAAGGAATTCAAAGGTGATCTTAAAGGCAAAAAAATTGTCTTTGCTGGAGACATCAAAAATAATGTTGCTCGTTCAATAATGATTGGTGCTGCTTTTACAGGAATGAACTGTGTTCTATGCGGACCTAAAGAACAAGCAAAAATTGTAAAAACTGGAAAAGGTTACAAAGAAGTTTACGAAAAATGTCTAGAATTATTCAAACGTAATGGTGGATCAATTACATTTGAAGACGACAAAATCAAAGCTGCTAAAGATGCAGATGCAATCTATACAGATGTTTGA", "aa": "MLADYMTIKEFKGDLKGKKIVFAGDIKNNVARSIMIGAAFTGMNCVLCGPKEQAKIVKTGKGYKEVYEKCLELFKRNGGSITFEDDKIKAAKDADAIYTDV", "aa_hexdigest": "83345eb2642fcf610372ace96df904cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.8514851485148515, "hmm_cov": 0.9186046511627907, "evalue": 1.3443774030420332e-18, "score": 63.5078125, "start": 16, "stop": 101 } ], "seq_stats": { "molecular_weight": 11120.914499999988, "isoelectric_point": 8.942103385925293 }, "id": "IGIGPGHMMC_878", "locus": "GCA000148625_00851" }, { "type": "cds", "sequence": "contig_1", "start": 486675, "stop": 487061, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAACTTAAAAGGTAGGAGTTTAGACTCAGCTCTAAATTTCACTACAGATCAAATTAATGCACTTATTGACCTTGCTATTGATTTAAAAAGATCAAAAAGACAAGGGTTACATGCTAACAATCGTCCACTTGTTGGAAAAAACATTGCTATCATGTTCCAAAAAGACTCTACTAGAACACGTTGCGCCTTTGAAGTGGCAGCAGCTGACTTAGGAGCATCTTGCACATATATCGGACCTGCAGGTTCTAACTTTGGTAAAAAAGAATCTATCGAAGACACTGCACAAGTTTTAGGAAGATTTTATGATGGTATCGAATTCAGAGGATTCAAACAATCTGATGTTGATGCGTTAGTTAAATATTCTGGAGTTCCAGTTTGA", "aa": "MPLNLKGRSLDSALNFTTDQINALIDLAIDLKRSKRQGLHANNRPLVGKNIAIMFQKDSTRTRCAFEVAAADLGASCTYIGPAGSNFGKKESIEDTAQVLGRFYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "7de699fd2dd1b14b4ad631581d29788d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.586, "score": 144.0, "evalue": 6.2e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 4.940699728336143e-31, "score": 103.83216094970703, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 13940.724999999984, "isoelectric_point": 8.974337577819824 }, "id": "IGIGPGHMMC_879", "locus": "GCA000148625_00852" }, { "type": "cds", "sequence": "contig_1", "start": 487152, "stop": 487415, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCTATCTTAATTCCTATCGCAGGTAAAGGAGCAAGTCAATTAGATATTGATATCGAAACTCACTTCGATGGTACAAACTACTTGACAATTGCACCTAGTGTTGTAGTTGGATATTCAAGAAATGAAAAAACTGAAAAAGCTCTTAAAGCAGCTAAAGTTAAAGTTTTATCATTCGAAGGAAACCAACTTTCATTAGGCATGGGTTCAGCAAGATGTATGTCAATGCCTCTTATTAGAGAAGACATTAAGAAAAAATAA", "aa": "MKPILIPIAGKGASQLDIDIETHFDGTNYLTIAPSVVVGYSRNEKTEKALKAAKVKVLSFEGNQLSLGMGSARCMSMPLIREDIKKK", "aa_hexdigest": "2f2c5d3fcf96a4992e30421335741837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9464.039899999998, "isoelectric_point": 9.373719215393066 }, "id": "IGIGPGHMMC_880", "locus": "GCA000148625_00853" }, { "type": "cds", "sequence": "contig_1", "start": 487563, "stop": 487661, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAAAATAGCAGCGGACAAAGAAGTTAAATTCAAAAGAATTTATGCAATCAATGTTCCTCCTATGCCAAACTTAATGCACTTAGATACATGA", "aa": "MARKIAADKEVKFKRIYAINVPPMPNLMHLDT", "aa_hexdigest": "c57cbf45ae18dee8a6fdae57809795db", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3712.4553000000014, "isoelectric_point": 9.819775962829588 }, "id": "IGIGPGHMMC_881", "locus": "GCA000148625_00854" }, { "type": "cds", "sequence": "contig_1", "start": 487773, "stop": 487988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTATCGACAAAAAAGAACTTGGTATCGAATTAGATAGAGATCTTGTAGTTGACCCTATGCCAAACTTATACTTTACACGTGATCCATTTGCATCAGTTGGTAATGGTATTTCATTACACCACATGAAATATGTCACAAGACAAAGAGAAACAATATTCTCAGAATTTATCTTTGACAATAACCTTGATTACAATACAGTACCTCGTTGA", "aa": "MAGIDKKELGIELDRDLVVDPMPNLYFTRDPFASVGNGISLHHMKYVTRQRETIFSEFIFDNNLDYNTVPR", "aa_hexdigest": "57e3b907be017ce5528ff33462303180", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8262.283799999994, "isoelectric_point": 5.127866172790527 }, "id": "IGIGPGHMMC_882", "locus": "GCA000148625_00855" }, { "type": "cds", "sequence": "contig_1", "start": 488094, "stop": 488366, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTAATGTTTACAGTGAATACGGAAAACTTAAGGAAGTACTTGTTCACACACCTGGAGACGAAATTAGGCGCATCGCACCTTCTCGCCTCGATGAACTATTATTTTCAGCAATTTTGGAACCAGATTCTGCAATTGCGGAACATAAAAGATTTGTTCAATTATTAAAAGATAACGGAATTAAAGTTATTCAATTGGACGAATTATTCGCTAAAACTTTTGACTTGGTTAGCGAATCAGTTAAGCAATCATTCATTGAAAGATGA", "aa": "MKKINVYSEYGKLKEVLVHTPGDEIRRIAPSRLDELLFSAILEPDSAIAEHKRFVQLLKDNGIKVIQLDELFAKTFDLVSESVKQSFIER", "aa_hexdigest": "d24a32e51bd2576a428d37b49047a9dc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10358.876999999991, "isoelectric_point": 6.101233863830565 }, "id": "IGIGPGHMMC_883", "locus": "GCA000148625_00856" }, { "type": "cds", "sequence": "contig_1", "start": 488662, "stop": 488808, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATGATTATGACATATGATTTTATTATGGAAATAGTAGCTAGTGTATTTCCTAAAATTTACGAAAACGAGTATAAATTAAAGGAAAACCAATATTATGATTTTGATCCTAATAGCTTAGAATTAGTAATCAAAAACAACTAA", "aa": "MAMIMTYDFIMEIVASVFPKIYENEYKLKENQYYDFDPNSLELVIKNN", "aa_hexdigest": "ed2ff75802bea58ddd481f1811b27e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5816.648800000003, "isoelectric_point": 4.333427619934083 }, "id": "IGIGPGHMMC_884", "locus": "GCA000148625_00857" }, { "type": "cds", "sequence": "contig_1", "start": 489016, "stop": 489597, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATCCTTATATTCAAATCACTCACCAAGTTTCAATTATTGAAACAATTAATGGACAAATAACAAATAAAAATAATATTGTTAAAGACCCTAAAAATATCACGATTTATGATCTTCTAGACAATATTATTGCTGTTTATTCAAATAAAGCTGACTACTACATTGTTTATAACAAAGGTTATGAAAATACCAGAAACAATGAACTTATAGAATTAGCTAAACTTTATTATGCTGATAGTCAACATAAAAAAGACATGGAACACTATTTATCTATAAATAAGCTCGATATGGAAAAATTAGAACAATTAATTACTTATGTTAATGAACATACTATTGACTTATTAGATTGTTTTAAACCAATATCTAAGAATAAATTCGATGAACGTTATGGCATGGATGCTAATAAAAATTATTATGTTTTTAAAACTAATTATGTCCCTCAAGCTAAATTTCATAGTGAAAATTTAAATAAAGAAATTTTATATACTTCAGTTTCTAAAAATAGTATTCCTTTTAACGTTGAAAATTTAGCAACTTACTATAAAGACGAAAGATTTTCAACTTTCATTTATGAATTATGA", "aa": "MNPYIQITHQVSIIETINGQITNKNNIVKDPKNITIYDLLDNIIAVYSNKADYYIVYNKGYENTRNNELIELAKLYYADSQHKKDMEHYLSINKLDMEKLEQLITYVNEHTIDLLDCFKPISKNKFDERYGMDANKNYYVFKTNYVPQAKFHSENLNKEILYTSVSKNSIPFNVENLATYYKDERFSTFIYEL", "aa_hexdigest": "5dc021c36ce31ad97f9460d4e05b8c47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 125, "aa_cov": 0.6217616580310881, "hmm_cov": 0.768, "evalue": 1.2113250730069214e-13, "score": 47.455665588378906, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 22937.642800000016, "isoelectric_point": 5.750025749206543 }, "id": "IGIGPGHMMC_885", "locus": "GCA000148625_00858" }, { "type": "cds", "sequence": "contig_1", "start": 489742, "stop": 490362, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTAAAACAACTAATAAAGATTATTTAGAAGCTAATTACTTATATCAAGTAGCTAAAAAAGCCAAAGTTGAAGTTAAAAAAATTCAAAATATTATTTTTGATGACTATACTCAAAATGGTTTTGTTTTTAGAAAAGATAAACTAAACTTAAGATTAATTGAAGCTTCAAGTTGTGGTAAAAACAAAATGGACAAAAAGGCTTTAAGAAGTGATCTTGGTTACACAATTAATGATGGTTCTTATTTTGCTTCAAAAAATGAAGCTAATGTTCATGGAGATTACCTTTTTGCCGCACTTCAAGAAGATATTTTCACTCAACCAAGTGCACGCAAAATGCCAAAAACTTCTTTAGAATTTATGAGCAAAGATCCAAGAAGTAAATTTGGAGACTTTAAAGAAGTAATAAGTAAAATTGTAGAAGCTTATTATTTAACTAAACCAACATTTTCATTTGATAATCAAATTATGTTGCCGGATTCAGATATGTTTTATGGTAAAAACAAAGATTTAATGAATAAAGTTAGAGTAGCTGTTACACCTCTTGAATTACAATATTCTCCTGCTGTCAAAAAATGTTTAAAAACTTTTGATCAAGAATACTTAAATCAATTTTGA", "aa": "MNSKTTNKDYLEANYLYQVAKKAKVEVKKIQNIIFDDYTQNGFVFRKDKLNLRLIEASSCGKNKMDKKALRSDLGYTINDGSYFASKNEANVHGDYLFAALQEDIFTQPSARKMPKTSLEFMSKDPRSKFGDFKEVISKIVEAYYLTKPTFSFDNQIMLPDSDMFYGKNKDLMNKVRVAVTPLELQYSPAVKKCLKTFDQEYLNQF", "aa_hexdigest": "32560bdf123e582d890723fb91325331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23845.065899999998, "isoelectric_point": 9.1501428604126 }, "id": "IGIGPGHMMC_886", "locus": "GCA000148625_00859" }, { "type": "cds", "sequence": "contig_1", "start": 491054, "stop": 491197, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAGCTGCATTCTTAAAAGAAGAAGACTACGCCGATCCTGAAAACTTTCCAATTAATGAAAGATATTTTGCAACTTATCCTTTTAAAGTTGATAAAAAAATGGCTGAAGATGATGCAAATACCATAATTGAATTTTGA", "aa": "MIAAAFLKEEDYADPENFPINERYFATYPFKVDKKMAEDDANTIIEF", "aa_hexdigest": "59a9b8a7fb826e66fc6d94f67f779798", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.117300000002, "isoelectric_point": 4.214918327331543 }, "id": "IGIGPGHMMC_887", "locus": "GCA000148625_00860" }, { "type": "cds", "sequence": "contig_1", "start": 491279, "stop": 491803, "strand": "-", "frame": 1, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E", "UniRef:UniRef90_UPI000FF8613E" ], "nt": "ATGAACAGAGTTAAAAAATTAACAAAAAGAAAATTCTATAATGGACAAGATTACATTTTAGATGAAAGAAAACAAACAATTAAATTAACAACCAATTTTCATAACACACTTTTAAATAGTTTAGGAACTTTTAATGGTTTCAAAAAATTTGGTGGTAGCTCAATTGGTAACGTCTTAGAAACAGATAGCTTCAAAAGTCATTTCCAAGCTTTTTGTTTTATGTCAAGATTAAGTATGCCAGTGTTACAAACTAAATATATAGATGCCGGGGTAGCTATAGAACCTAAAGTTTTTGAACTTATGCAAAATAAATTTAATGAAATGCATCAAAAAGATATTAAAAGAGGTAAAGTTAGTGCTGATAGTCAACCAACAAAAGTAATTCATATTGAAGCAGCAAATGTCGGATATGACTATTTTAAAGGCAAACACGACATTTTAGGTGGTGTTCCTGATGGTCTTATTCCTTCAAAAAATATTGTAGTTGAAGTTAAAACTGTTCAAGAAAAGAAAAAGGTTGATTGA", "aa": "MNRVKKLTKRKFYNGQDYILDERKQTIKLTTNFHNTLLNSLGTFNGFKKFGGSSIGNVLETDSFKSHFQAFCFMSRLSMPVLQTKYIDAGVAIEPKVFELMQNKFNEMHQKDIKRGKVSADSQPTKVIHIEAANVGYDYFKGKHDILGGVPDGLIPSKNIVVEVKTVQEKKKVD", "aa_hexdigest": "5ee56e127bb1e91fcf8a2a23d9b46c64", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF8613E", "query_cov": 1.0, "subject_cov": 0.8877551020408163, "identity": 0.971, "score": 336.0, "evalue": 3.06e-116, "valid": true, "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_UPI000FF8613E" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF8613E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E" ], "product": "MAGa7180 family putative nuclease" }, "genes": [], "id": "IGIGPGHMMC_888", "locus": "GCA000148625_00861" }, { "type": "cds", "sequence": "contig_1", "start": 491993, "stop": 492769, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7", "UniRef:UniRef90_C4XEY7" ], "nt": "ATGCCTTATAAAGCTATTATTCAAAAAATATCTGAATGTGAAGAAAATAAAACAAATGTAACAAGTGTTAAAGAATTAGAAAAAATTAATGTTGAAGATGTCATTAGAATCGACGATGAATTTTTAGATCAAAAAATATTCAACAATAAAAACTTCTTATTTTTCGATTTTGAATCTAGATTAACAAAATTAAATGATAAAGTGGCAATTAGTTACTATCCAGTTCAATATTCTTCTTTTATATCAAACTTAAACAATAAAGAATTTAATGAAAAAGAAAGAAAAAACTTTTACAAAGGTGTAGTTAAAGACAACCGTAAGTTTTTCCAAATTGATGGTTTCATTTTAGATCAATATAGCAATATTTATCAAGATCAAAGTCAAAATTTAATAACAAGAGAAGAAGTTCAAAATATTTTAGATGCTAACAAAATCTTAATTTCTTATGGCATTAAAGCCGATTTAAACAACTTGAAAAAAATTGTAGGTAAAAAATATGTTGAAAGTCGATTAGTCAATTATATTGATGTTTGCAATTTTACAATGATAATTAAAAAATTAATTAGTAAAGAAAAAGAAACAATGTATAGACAAACATTATTTAATGTGGCAAAATATTTTTGTACTTATGATGATGCTTTACTTAGTGAAGAATTAAAAGAAGAATTAAAAAATAAAGATAAAAAAATAAATATACTTTTTCAAGAAGAATATAAATTTGAAAAATTGAATATGCATGATTCATTTGAAGATTCAAGAATCTTAACAATTCTTTGA", "aa": "MPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTIL", "aa_hexdigest": "b03ec4737b699f792aeab9e6971cef24", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 491816, "stop": 493069, "observations": { "mutation": [ 491857, 491995, 492778 ], "stop": [ 491857, 491995, 492778 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEY7", "query_cov": 1.0, "subject_cov": 0.5720620842572062, "identity": 1.0, "score": 494.0, "evalue": 1.35e-173, "gene_start": 1, "gene_end": 258, "reference_start": 135, "reference_end": 392, "reference_sequence": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKNWHKMPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTILWNNVVHRFLQRELCNNLFYKKSIDDNDSKVEKRIETFRDLIFAIKYWLDKIYKEENSTN" }, "paralog": false, "identity": 0.9928057553956835, "score": 821.0, "evalue": 7.11e-305, "description": "Internal stop codon at 491857, 491995, 492778. Nonsense mutation around 491857, 491995, 492778.", "psc": { "uniref90_id": "UniRef90_C4XEY7", "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEY7", "UniRef:UniRef50_C4XEY7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30831.941400000054, "isoelectric_point": 6.098789787292478 }, "id": "IGIGPGHMMC_889", "locus": "GCA000148625_00862" }, { "type": "cds", "sequence": "contig_1", "start": 492776, "stop": 493171, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAATATAAACGGAACAATTAAAAAGACTTTTATCGAAGTTATTGAAGAATTAGAAAATAACCCAAGTATTTTTTCTAAAAAACAATGTTATGAAACACAAGGCTACATATTTAATAAAAGCTTAACGGCTGATAAAGATAATAATCAATTTATTTCTTTAATAGTTGGAGATGAAATTGATACTAATGATTTCTTTGAACTTAAATTCTTTTATTATCCAAAAGATAAAAATAAAACTGATAAAGAAAAATCATATTCTGTAATTAAAAAAATATTTGGTTTCAAAGTACAAACTAAAACTAAACAAGAAAAACAAAATGAAGCAAACAAAAGACAAAACGATTTAGTTAAATATAGTTTTGTTTATACAGTTTCAAAAAATAAAAATTGA", "aa": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKN", "aa_hexdigest": "993e24a5051a319a611a075aabe5fd88", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15458.295499999987, "isoelectric_point": 9.128030204772951 }, "id": "IGIGPGHMMC_890", "locus": "GCA000148625_00863" }, { "type": "cds", "sequence": "contig_1", "start": 493287, "stop": 494042, "strand": "-", "frame": 3, "gene": null, "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961", "UniRef:UniRef90_A0A3P8LBF2" ], "nt": "ATGAAAGGTAAAAGAGTTGTTATTAGAACACTAGATATTGGTGGTGATAAAACTCTTAAATACTTTACATTCCCTCACGAAATGAACCCATTCTTAGGTTATCGTGCAATAAGACTTTGTCTTAAAGAAATTGAAATCTTTAAAACTCAACTTAGAGCTTTAGTTAGAGCAAGTGCTTATGGTAAAGTTGCTATTATGTTCCCAATGATAACAAACATTCCAGAATTCTTGCAAGCTAAAAAACTTTATGAAGAAGCTTATAGTGAAGTTAAAAAAGCAGGACACAAAATTGCTCCTAAAAAGGATATTGAAGTAGGTCTTATGATGGAAACCCCTGCTGCTGCTGTATTAAGCGATCAATTCTGTAAATATGCAGACTTTGTTTCGATTGGAACTAATGACTTAATTCAATACTCAATGGCTGCAGATAGAATGAGTGAAACTATTTCATACCTATATCAACCGCTTAACCCTTCTATTTTAAGATTAGTAAAAATGGTTATTGATGGTGCTCACAAACATGGTAAATGGGCTGGTATGTGCGGTGAAATGGCCGGAGATAGACGCGCATTGCCTTTACTTTTAGGTTTAGGTTTAGATGAATTTAGTATGTCTGCTGGTAATGTTTTAGCTTCAAGAGAATTGGTATCTCAATTAAGCAAAAAAGACATGGAAGAATTGGCTGCTCAAGCTATTGAATTAGATAGTGAAGAACAAGTTATTAAACTTTTAAATAAAGCTTTAAAATTAAAATAA", "aa": "MKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK", "aa_hexdigest": "421008abf5a5322aa455ec99bbb5cc7b", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FF43100", "query_cov": 0.9920318725099602, "subject_cov": 0.8861209964412812, "identity": 0.759, "score": 377.0, "evalue": 9.49e-130, "valid": false, "product": "phosphoenolpyruvate--protein phosphotransferase", "uniref50_id": "UniRef50_A0A938YW30" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 493287, "stop": 494342, "observations": { "mutation": [ 494093 ], "stop": [ 494093 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBF2", "query_cov": 1.0, "subject_cov": 0.42327150084317033, "identity": 1.0, "score": 483.0, "evalue": 3.01e-167, "gene_start": 1, "gene_end": 251, "reference_start": 343, "reference_end": 593, "reference_sequence": "MKSHKRLYVYIIKLHTYLNKMEVKMTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDPWALETITGRIKDNKETAEYATKAFYEEMATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEHWPTEEEQFAAYKEVVERMKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK" }, "paralog": false, "identity": 0.9971509971509972, "score": 687.0, "evalue": 1.29e-249, "description": "Internal stop codon at 494093. Nonsense mutation around 494093.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBF2", "product": "Phosphoenolpyruvate-protein phosphotransferase", "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBF2", "UniRef:UniRef50_W6A961" ] }, "pscc": { "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "IGIGPGHMMC_891", "locus": "GCA000148625_00864" }, { "type": "cds", "sequence": "contig_1", "start": 494091, "stop": 494681, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00391.29", "PFAM:PF02896.24" ], "nt": "ATGGCAACAATGTTTTCAAATATGGACGATGCCTACATGAAAGAAAGAGCTGCTGATGTTAAAGATGTTATGAAAAAACTTCTTTACATTTTAAATGACATTGAAGAACCTGATTTAACTGCTATTGATAAAGAAGTAATTATTGTGGCTTATGATTTAAGTCCTTCTCAAACTGTTCAATTAAATAAAAAATATGTTAAAGGTTTTGCAACCAACATTGGTGGACCAACAAGTCATACAGCAATTATGGCTAGAAGCATGAACATTCCTTCAGTAGTTGGAACAAACAATGTTTTAGAATCAGTTAAAAATGGTCAAACAATTATTATTGATGGAATCAAAGGCGAAGTAATTGTTGATCCTACAAAAGAAGAATCAGAACACTATAAAAAAGAAGAAGCTAAATACATTAAATATTTAGAAACAATTGCTAAATACAAAGGTAAAAAATCAATTACTAAAGATAAACATCATGTTGAATTAGCTGCAAATATTGGACGTCCAAAAGATGTTGATGCAGTTATTGACAATGATGCTGAAGCAATTGGGTTATTCCGTTCAGAATTCTTATACATGGATAATGAACACTGA", "aa": "MATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEH", "aa_hexdigest": "61080fa331e7a2447a8542b4409ab0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00391.29", "name": "PEP-utilizers", "length": 73, "aa_cov": 0.37244897959183676, "hmm_cov": 0.9726027397260274, "evalue": 1.3107760926419453e-26, "score": 88.05028533935547, "start": 43, "stop": 115 }, { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 52, "aa_cov": 0.2653061224489796, "hmm_cov": 1.0, "evalue": 2.824795712230862e-13, "score": 45.6678466796875, "start": 144, "stop": 195 } ], "seq_stats": { "molecular_weight": 21956.865300000012, "isoelectric_point": 5.436558723449706 }, "id": "IGIGPGHMMC_892", "locus": "GCA000148625_00865" }, { "type": "cds", "sequence": "contig_1", "start": 494760, "stop": 494996, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAAAGGAATCGGAGCAAGTCGTGGAATCGCGGTTGCAAAAGTTTTTAAAATTGAAGAACTACCAGTCAATATTACTGAAAAAGCTAAGGATGCAGATCATGAATTAAAACTATATAATGATGCTGTTAAAAAAGCTGGTGAAAAAGTTGAAGCTACTAAAAAATTAGCTAAAACTCCTGAACAAGCTGCTATTTTTGATGCGCATTTACAAATTATTAATGACCCTTGA", "aa": "MTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDP", "aa_hexdigest": "daf661ae6e2f783cd7f62229f659d7f7", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8483.747499999998, "isoelectric_point": 7.969017601013183 }, "id": "IGIGPGHMMC_893", "locus": "GCA000148625_00866" }, { "type": "cds", "sequence": "contig_1", "start": 495238, "stop": 495381, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTAGCTGCTGATGTTCATGCGCCTAAGGATTTAAGAGCAAATATTCAAGCTAAAAACTTAGATGAATTTCATGTAACATTTAAAACAAAACCTAGTGATAAGATGTATTTAGCACCTGAAAAAAGAGTCAAAATCTGA", "aa": "MLLAADVHAPKDLRANIQAKNLDEFHVTFKTKPSDKMYLAPEKRVKI", "aa_hexdigest": "2456438a79a605ac355e2e761026d5e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.290500000001, "isoelectric_point": 9.52199649810791 }, "id": "IGIGPGHMMC_894", "locus": "GCA000148625_00867" }, { "type": "cds", "sequence": "contig_1", "start": 495424, "stop": 495582, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATCTTTTTGAAGGCCAAGATTCAGAATTTGGTAAATGTAATGGTAAATTAACTGTATCTGAAAATATTGCTGATGCTGGTGGAATTAGATGTGCATTAGAAGCTAGTCAAAAAGAATTAAATCATTCATCAAAAGAATTCTTTACAAACTGA", "aa": "MVDLFEGQDSEFGKCNGKLTVSENIADAGGIRCALEASQKELNHSSKEFFTN", "aa_hexdigest": "b883c3b01f53be6c7dc51cb7daaa4681", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5667.191499999998, "isoelectric_point": 4.638823509216309 }, "id": "IGIGPGHMMC_895", "locus": "GCA000148625_00868" }, { "type": "cds", "sequence": "contig_1", "start": 495628, "stop": 495837, "strand": "-", "frame": 2, "gene": null, "product": "M13-type metalloendopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "nt": "ATGTCACCTGCTGAAGTGAATGCTTACTATAATCCTTTTATGAATCATATAGTTTTCCCTGCTGGGATTTTAGCAAAACCATTCTACTCATTAACCCAAAGTTCTTCAGCTAACTATGGTGGAATTGGAGCTGTTATTGCTCACGAAATTTCACATGCTTTTGATAATAATGGAGCTAAATTTGATGAAAAAGGTAATTTAAATTCATGA", "aa": "MSPAEVNAYYNPFMNHIVFPAGILAKPFYSLTQSSSANYGGIGAVIAHEISHAFDNNGAKFDEKGNLNS", "aa_hexdigest": "4d0f0ef07347504266be0d5db2a78593", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025523856", "query_cov": 0.927536231884058, "subject_cov": 0.8205128205128205, "identity": 0.516, "score": 72.4, "evalue": 2.42e-15, "valid": false, "product": "M13-type metalloendopeptidase", "uniref50_id": "UniRef50_UPI0025523856" }, "pscc": { "uniref50_id": "UniRef50_UPI0025523856", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "product": "M13-type metalloendopeptidase" }, "genes": [], "id": "IGIGPGHMMC_896", "locus": "GCA000148625_00869" }, { "type": "cds", "sequence": "contig_1", "start": 495841, "stop": 495960, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAACTCCATATAAACATGAAGGTAATTTAGTAGTTAATGCATTAAATCTTAATGCAATTGAAAGCGAATATAACTTTAGTCAATACAAACAACCAATTAATAAAAATTTATGA", "aa": "MVITPYKHEGNLVVNALNLNAIESEYNFSQYKQPINKNL", "aa_hexdigest": "f06ea9320b2c4b85cf7627aa8624f18b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4508.071700000001, "isoelectric_point": 6.519455146789551 }, "id": "IGIGPGHMMC_897", "locus": "GCA000148625_00870" }, { "type": "cds", "sequence": "contig_1", "start": 496057, "stop": 496326, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTAGAAACTTAATTAGATTTTCAAATCTTTTGGACAATAAAACTAGAATTACTGCAGGAGAATTTAACCGTGCAGTAAGTGGAATTGATAAAGCTTCAAGTGCAGCTAAAAGTGCTTTCTACTTAGCTTATTCATACTTTAAAATTCCTTTTGGTTTAAGATTTGCACTTGAAAATTTTGGTCCTGAAGCTAAAAGAAATGTTGAATATATGGTTCAAAAAATGATCAAAATTTATCAAGACCGTTTAAGAAACAACAGCTGA", "aa": "MYLRNLIRFSNLLDNKTRITAGEFNRAVSGIDKASSAAKSAFYLAYSYFKIPFGLRFALENFGPEAKRNVEYMVQKMIKIYQDRLRNNS", "aa_hexdigest": "a133bad68f540abfca27b32ddb24981f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10353.833199999994, "isoelectric_point": 10.05502109527588 }, "id": "IGIGPGHMMC_898", "locus": "GCA000148625_00871" }, { "type": "cds", "sequence": "contig_1", "start": 496327, "stop": 496794, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTGATTTTGAAGTAATGCAAGATTTTAAAAATACCGAATTACAAACTTTAGGTTTTAGTGGACCAAGTTTAATTTTGCCAGAAAAATCATATTATGATGACAATCATCCAGCTAAACAACCGCTTTTAGAAGTATTTAAAAACATGTCAAGAAAATTATTAGCTGCTTATTTTAAAGATAAAAATAAAATTGAAGATATTATTTCTAATACATTAGAATTTGATAACTCATTAGTTAAATACACAATGAGTGCAGTTGAAAGTGCTGACTATCCCTCTTTATATAATCCTTTTACAATTGATGAAATTAATGAACATTCAAAAGTTTTTGATCTTAAAGAAATAGCAGAAAGATTAATTAAACAAGAAGTTAGCGAAAAAGTTGTTGTAATTTATCCTAAATTTATGAAAGGTTTAAATAGCTTATATAACTCAAGAACATTTGAAACATTTAAATGCTGA", "aa": "MPFDFEVMQDFKNTELQTLGFSGPSLILPEKSYYDDNHPAKQPLLEVFKNMSRKLLAAYFKDKNKIEDIISNTLEFDNSLVKYTMSAVESADYPSLYNPFTIDEINEHSKVFDLKEIAERLIKQEVSEKVVVIYPKFMKGLNSLYNSRTFETFKC", "aa_hexdigest": "9b945939e30db9d4665034f7a33c910f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18076.451199999996, "isoelectric_point": 5.122978019714354 }, "id": "IGIGPGHMMC_899", "locus": "GCA000148625_00872" }, { "type": "cds", "sequence": "contig_1", "start": 496858, "stop": 497010, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGATTTAGTTAGAACTCGTGATGCAGGTGAATTAGAAGTTGGAGCAATTTTAAACTTCACAAATCTTTATTCAATGGCTAGTGATTTTACTTTTAGAAATAAATCTAAAGTTAAACCACTTAAACCTTACTTAAATAAAATTTGA", "aa": "MASDLVRTRDAGELEVGAILNFTNLYSMASDFTFRNKSKVKPLKPYLNKI", "aa_hexdigest": "0fb247e5823063ec4eb8a7e71f994ff8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5664.5136, "isoelectric_point": 9.599229621887208 }, "id": "IGIGPGHMMC_900", "locus": "GCA000148625_00873" }, { "type": "cds", "sequence": "contig_1", "start": 497218, "stop": 497646, "strand": "-", "frame": 2, "gene": null, "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "EC:2.1.1.193", "KEGG:K09761", "SO:0001217", "UniRef:UniRef50_A0A449B7G8", "UniRef:UniRef90_C4XEZ0" ], "nt": "TTGCAAAAAGCTACTGAATTAGGAGTTACTACAATTATTCCTTTTGCCTCTAAATATACTGATGCAAAAATCTTAAAATATGAATTCAACAGTAAGCAAAATAGGTTTAATGAAATCATTAAAAATGCAGCTGAACAAAGCTTTAGAAACATCATTCCTTATTTAACTAAAATTATGAATTTAGAAGAAATTATTAAGACTTATCCTACTATGCAAAAGTACTTAGCTTATGAAAATCAAGACCAAAAAAATAACATTATCACTTATCCAACTAGTTCAATTTTAATAGTTGGACCTGAAGGTGGTTTTAGTCCTGAAGAAATAGAATTAGCTAAAGAAAACAATATTAAAATTACTTCATTAGGCAAAACAATCTTACGTGCTGAAACAGCTTGTTTGTATATGCTAGCTAATATCAAAGAAAAATAA", "aa": "MQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK", "aa_hexdigest": "2246d4ae69939870fd27bd08e12df65f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 497218, "stop": 497916, "observations": { "mutation": [ 497652 ], "stop": [ 497652 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEZ0", "query_cov": 1.0, "subject_cov": 0.6120689655172413, "identity": 0.993, "score": 280.0, "evalue": 3.54e-94, "gene_start": 1, "gene_end": 142, "reference_start": 91, "reference_end": 232, "reference_sequence": "MNERNKMHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFEWILQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK" }, "paralog": false, "identity": 0.9913793103448276, "score": 456.0, "evalue": 3.8e-166, "description": "Internal stop codon at 497652. Nonsense mutation around 497652.", "psc": { "uniref90_id": "UniRef90_C4XEZ0", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A449B7G8", "kegg_orthology_id": "K09761", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEZ0", "EC:2.1.1.193", "UniRef:UniRef50_A0A449B7G8", "KEGG:K09761" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7G8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7G8" ], "product": "Ribosomal RNA small subunit methyltransferase E" } }, "id": "IGIGPGHMMC_901", "locus": "GCA000148625_00874" }, { "type": "cds", "sequence": "contig_1", "start": 497650, "stop": 497898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGATTTTTTGTTACTAAAAAAAGTGATCAAGATCATTTTGAACTATCAAAAGAGATATTAAATCACATCAAAGTTGCACGTGTTGAAAAAGATGAATTTTTATGTAACTATCAAGACATTTTTTATAAGTGCAAATTAGAAAATAATAAAGCTTTAATTATCGAAAAATTAGATATAAATCATGAATTTAATAAGCCAATTATTCTTGCTGCACCAATTATTAAAATTAAAAGATTCGAATGA", "aa": "MHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFE", "aa_hexdigest": "da417bb2bf34f539d5efe3f686e7eaf7", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9904.495299999988, "isoelectric_point": 8.566639518737794 }, "id": "IGIGPGHMMC_902", "locus": "GCA000148625_00875" }, { "type": "cds", "sequence": "contig_1", "start": 498063, "stop": 499211, "strand": "-", "frame": 3, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "GO:0000910", "GO:0003924", "GO:0005525", "KEGG:K03531", "RefSeq:WP_013354660.1", "SO:0001217", "UniParc:UPI0001E33107", "UniRef:UniRef100_C4XEZ1", "UniRef:UniRef50_C4XEZ1", "UniRef:UniRef90_C4XEZ1" ], "nt": "ATGGACTTAAATGCTGAAGATTTGGAAGTTAAACTAAAAGTCATTGGTGTTGGTGGTGCAGGTAATAACGCCATTAACTTGATGCTTGATGAAAACTTGCCAAATGTTGAATTACTAGTTGCTAACTCAGATCGTCAAGATTTGGTTAAAAGCTTATGTCCAAATAAAATACTTTTAGGTGATTCTACCAGAGGCTTTGGTGCTGGTGGGGACCCTAAAGTAGGACGTGAGTGCGCACTAGAAAGCATTAAAGAAATTCAAAAATCATTAGAAAACACAGATATTGTTATTATTTCAGCTGGCTTAGGCGGCGGAACAGGAACTGGAGCAGCACCAGTGATTGCCGAAGCTGCCAAAAAGATGGGCATTTTGACAGTGGCAGTAGTAACCACTCCTTTTGAATTAATTGAAGGAAAACATAAAAGCTTAATTGCTCAAGAAGGCTTGAAAAAATTAAGTGAAGTTGTTGATAGTTATATTGTAATTTCAAACCAAAAATTGGTTGAAAATTACAGAAACTTACCAGTTCAAGAAGCTTTTAAAGTTTCTAACTATACATTGAAAAATTCAATCAAAATAATTCGTGATATTATCTTTGAAACTGGGTTTATTAATTTAGATTTCAATGATTTACGTCAAGTTTTATTAGATGGAAAAGAAACAATTATAGGAATTGGTAATGGCTTTGGCAAAGACCGAGCTATTAAAGCAGTTGATGATGCTTTAATGACTCCACTTTTTCAAAGTGAAATTAAAAATTGTCAAAAAGTTGCTATTTTATTTCAGTGTGATAAAAGAGCAAGTTTAGATGACATTGAAACAGCAAAAAATCGAATTGATGAGTATTTAGCAAATAACCTTGAAGCTCAAACTTTTATCGGACTTCAATATATCGATACTCAAGATCGTGAAGAAATATTCAGAATTAGTATTATTGCTTCAAATCTTAATGCAAATGTTTCAACAAATACTTATGCGGTACCAAAATTATTGCAAAGAAATGTTAGTGAACATTATGAGCAAAATATGCAAAATACATATACACAAATTAATGGATCACATCATACTCGTATAATGAGTTATACTGCTCAAAACTATGAAGATGAAAATGAAGATGTAATGCCAAATTTCTTTGATGAAAATCATTAA", "aa": "MDLNAEDLEVKLKVIGVGGAGNNAINLMLDENLPNVELLVANSDRQDLVKSLCPNKILLGDSTRGFGAGGDPKVGRECALESIKEIQKSLENTDIVIISAGLGGGTGTGAAPVIAEAAKKMGILTVAVVTTPFELIEGKHKSLIAQEGLKKLSEVVDSYIVISNQKLVENYRNLPVQEAFKVSNYTLKNSIKIIRDIIFETGFINLDFNDLRQVLLDGKETIIGIGNGFGKDRAIKAVDDALMTPLFQSEIKNCQKVAILFQCDKRASLDDIETAKNRIDEYLANNLEAQTFIGLQYIDTQDREEIFRISIIASNLNANVSTNTYAVPKLLQRNVSEHYEQNMQNTYTQINGSHHTRIMSYTAQNYEDENEDVMPNFFDENH", "aa_hexdigest": "61bfc8f8ae42dae9f9463bea5f770824", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33107", "ncbi_nrp_id": "WP_013354660.1", "uniref100_id": "UniRef100_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33107", "RefSeq:WP_013354660.1", "UniRef:UniRef100_C4XEZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ1", "uniref90_id": "UniRef90_C4XEZ1" }, "psc": { "uniref90_id": "UniRef90_C4XEZ1", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_C4XEZ1", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ1" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "IGIGPGHMMC_903", "locus": "GCA000148625_00876" }, { "type": "cds", "sequence": "contig_1", "start": 499296, "stop": 499592, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCTTTATTTGACCAAGCTATTCATAAAGTTAAAGCGTTTTTAGACCAAAATAATCAAAAAGTTGATGAGTTGGTTTTAAACTGCAAAAACTCAGAAATCTTTATGGATCGTTTTAACAAATTATATCCCGAAATGAATGTTGCAATTCTAGACGATAACGTCTCAACTTTATTCAATATACCTAATAGCCTTTTAGGCATTATTAATTTACTAAATCCTGAAAAAATCAATTGTGAAGACATGGTTAATACTTTGAATAATTTACCAGAATACAAACCAAAAAGTTTTTGA", "aa": "MISLFDQAIHKVKAFLDQNNQKVDELVLNCKNSEIFMDRFNKLYPEMNVAILDDNVSTLFNIPNSLLGIINLLNPEKINCEDMVNTLNNLPEYKPKSF", "aa_hexdigest": "bd784c4c0456baab6732eb2f6dcc214c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11317.950999999986, "isoelectric_point": 4.780182075500488 }, "id": "IGIGPGHMMC_904", "locus": "GCA000148625_00877" }, { "type": "cds", "sequence": "contig_1", "start": 499647, "stop": 499778, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAAAACATTTTTCTATGAAAAATCTAACATTGACTTTAAAAACTTTGTTAATTATTTATCAAAAGAATTAAATAAAAGTAAAGAAGATACAATTTTGTTACTTGAAGCTGTGGTAGCTAATTGA", "aa": "MNAKTFFYEKSNIDFKNFVNYLSKELNKSKEDTILLLEAVVAN", "aa_hexdigest": "56f21b868eee47f2cdce876dd7497f23", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5030.702700000001, "isoelectric_point": 6.089979743957519 }, "id": "IGIGPGHMMC_905", "locus": "GCA000148625_00878" }, { "type": "cds", "sequence": "contig_1", "start": 500121, "stop": 500429, "strand": "-", "frame": 3, "gene": null, "product": "MAG3720 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D", "UniRef:UniRef90_UPI000FEECA7D" ], "nt": "ATGAAAAAAATTTATGTAAATTTCTTTCTTAAAAAAACTAGACTTGATGTGACAGTAGTGGAAGATATTAATTCTAAATATTTTCCAGTTTTTCAAACATATCTAGAAACCAAAAACTTTGAAGCGAATAAGATTAAAGAATTTATTACGAATTTCAAAAAATATTTGAAATCTAAAGTTGCTACCAAGAAAAATGATGTAGTTAAATATTCTTTTATAGTTAAAGATAGTTTATACAAAAGTTTAAAATTTGTTAACCAAACTTTGACTTTTGATGTTCCAAGTAATACAGTTGATCAACAAGTATGA", "aa": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQV", "aa_hexdigest": "36e174daa2dba9331720acc57107e6a9", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 499966, "stop": 500429, "observations": { "deletion": [ 500015 ], "mutation": [ 500123 ], "stop": [ 500123 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECA7D", "query_cov": 1.0, "subject_cov": 0.6623376623376623, "identity": 1.0, "score": 199.0, "evalue": 6.97e-64, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQVWKEVKNKLEEQKESSTYELINSNIYLYETINNDVVKKLQYFSLKQTSSKIKY" }, "paralog": false, "identity": 0.9935064935064936, "score": 294.0, "evalue": 8.56e-104, "description": "Internal stop codon at 500123. Frameshift due to deletion around 500015. Nonsense mutation around 500123.", "psc": { "uniref90_id": "UniRef90_UPI000FEECA7D", "product": "MAG3720 family protein", "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECA7D", "UniRef:UniRef50_UPI000FEECA7D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D" ], "product": "MAG3720 family protein" } }, "id": "IGIGPGHMMC_906", "locus": "GCA000148625_00879" }, { "type": "cds", "sequence": "contig_1", "start": 500479, "stop": 501441, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "EC:2.1.1.199", "GO:0016434", "KEGG:K03438", "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_A0A3P8MEW3" ], "nt": "ATGACAGTGACATATCTTCAGCAGCACAAAAAATTCTTGAAAGAAGTGGTAAATAAAATGGAGAATCAACACTTTTCTGTTTTACTGCAAGAGTCTATTGATGCCCTTAATATAAAACCAGATGGTATTTATGTTGATTTAACTTTGGGCATGGGTGGACATTCTAGCGAGATTTTAAAGAGACTTGACACTGGTCGTTTGATTGCTTTTGATAAGGACACTTTTGCGATAGAAGAAAGTCGCAAACGTCTCAGCCAAATAAAATCAACTAATTTTCAACTAATTCATTCTGACTTCAAAAATATAAAAAGTGAATTAGATAAATTGAACATTAGCCAAGTAGATGGAATCATTGCTGATTTAGGTATTAGCAGTCCACAAGTTGATAATGATGAACGAGGTTTCTCTTATAACAAAAATGCACGTCTTGACATGCGCATGGATCAAAGTCAAAAGCTTGATGCCTACTATGTAGTTAATAATTATAGTGAAAGTCAATTGGAACAATTATTAAGAAACTACGCTGAGGTAAAACTTGCTCGTCAGGTTGCGAAGGCCATCATTAGTAAGCGGCCAATAACTACCACTTTGGAATTAGCTCAAGTAGTGCGAGATGCTTATCCTGCAAAATTAGTGAAACTAAAAAATCCTAATAAAGCAGTGTTCCAAGCTATCCGTATTGAAGTTAATAATGAATTTGATTCCCTTAAGCAAATGTTACAAGATGCTATTGAACTATTAAAACCTAATACTTCTTTAGCCGTTATAACTTTTCATTCATTGGAAGACAGCATTGTTAAACATTTTTTTGGTTCACTAATTAAAAGTAAATTACCAGCAAAAATGCCAATTAATGAAGAGAAGCAATATAAAGTAAAAGTATTTCTTCCAACCAATAATGAGTTAGAACTTAATAAACGCTCTCGTAGCGCCAAGTTAAGAGTTTTGAAAAAATGCTTTTAA", "aa": "MTVTYLQQHKKFLKEVVNKMENQHFSVLLQESIDALNIKPDGIYVDLTLGMGGHSSEILKRLDTGRLIAFDKDTFAIEESRKRLSQIKSTNFQLIHSDFKNIKSELDKLNISQVDGIIADLGISSPQVDNDERGFSYNKNARLDMRMDQSQKLDAYYVVNNYSESQLEQLLRNYAEVKLARQVAKAIISKRPITTTLELAQVVRDAYPAKLVKLKNPNKAVFQAIRIEVNNEFDSLKQMLQDAIELLKPNTSLAVITFHSLEDSIVKHFFGSLIKSKLPAKMPINEEKQYKVKVFLPTNNELELNKRSRSAKLRVLKKCF", "aa_hexdigest": "ca69d16297885b96282fb4baac62a1bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C25A", "uniref100_id": "UniRef100_C4XEZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ3", "uniref90_id": "UniRef90_A0A3P8MEW3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEW3", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "kegg_orthology_id": "K03438", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "IGIGPGHMMC_907", "locus": "GCA000148625_00880" }, { "type": "cds", "sequence": "contig_1", "start": 501479, "stop": 501607, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATACTCAAGAAATTGAACTTGACAACCAAGGACGTTTCGTTGTTCCAAAACAATTCCTCGACAAAGCTGCTATCCAAAAAGAAGCCATTTTTGTAGCCTCAGGTAATCTTGTAGAACTTTGA", "aa": "MSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVEL", "aa_hexdigest": "e6c6179a47da3362b311faa7d14a7294", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4692.305799999999, "isoelectric_point": 4.655875205993653 }, "id": "IGIGPGHMMC_908", "locus": "GCA000148625_00881" }, { "type": "cds", "sequence": "contig_1", "start": 501608, "stop": 501820, "strand": "-", "frame": 1, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44", "UniRef:UniRef90_A0A3P8KXP2" ], "nt": "ATGTTTGGACAATACAAAAGAAACATTGACGACAAAAAAAGATTAGCTTTACCATCTAAATTGCGTGACGATTTAGGAAGCAAAATTTATTTAACTGTCGGACTTGAAGGCATTGTTGAAATTAGAAGCGAAAAAGAATTCAATAAATTCGTTGAAGTTCTAAATCAACAAAACTCTTTTGATACAAATGCTCGTTTTGTTAGAAGATTTTGA", "aa": "MFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRF", "aa_hexdigest": "fc1bcb8076e3e37f9a1362ad29c21bbf", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "mraZ" ], "truncated": "5-prime", "pseudogene": { "start": 501386, "stop": 501850, "observations": { "mutation": [ 501481, 501610 ], "stop": [ 501481, 501610 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KXP2", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 1.0, "score": 142.0, "evalue": 6.29e-42, "gene_start": 1, "gene_end": 70, "reference_start": 11, "reference_end": 80, "reference_sequence": "MVVDSGRKWKMFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRFWLSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVELWGSEKFDQYCQNFNDSDISSAAQKILERSGK" }, "paralog": false, "identity": 0.9805194805194806, "score": 295.0, "evalue": 6.51e-105, "description": "Internal stop codon at 501481, 501610. Nonsense mutation around 501481, 501610.", "psc": { "uniref90_id": "UniRef90_A0A3P8KXP2", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KXP2", "UniRef:UniRef50_F9UK44" ] }, "pscc": { "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44" ], "product": "Transcriptional regulator MraZ" } }, "id": "IGIGPGHMMC_909", "locus": "GCA000148625_00882" }, { "type": "cds", "sequence": "contig_1", "start": 502064, "stop": 502741, "strand": "-", "frame": 1, "gene": null, "product": "Potassium uptake protein A", "db_xrefs": [ "RefSeq:WP_013354664.1", "SO:0001217", "UniParc:UPI0001E3310B", "UniRef:UniRef100_UPI0001E3310B", "UniRef:UniRef50_C4XEZ5", "UniRef:UniRef90_C4XEZ5" ], "nt": "ATGCCTCGTAAAAGAAAAAATGAAGATATCTGTGTTATTGGAACAGGGCGTTTTGGTAGTGTGGTTGTAAGCCAACTTGCTAAAATGAATTGTCCTTTATTATTAATAGATAAAGATGAAGAAGTTTTAAAATCATATGCTGATTTAGCACAAAAAGTTGTTATTGCTGATGCAGGTGATATGAAAGCTTTAAGAGCACTTAATCTTCAAGAAATTGGAACTGTTGTTGTTGCTGTTCCTGATAATATTGAAATTGTGGCTGCTTTACTCGAATTAAAAGTTAATAATATTATTGCTCGTGCAACAAGTAAAAGACATGCACGTGTATTAAAACAAATTGGAGTTAATGTTATTGTTAGATCTGAATATGAAGCCGGTGTTCGTACTGCTCTTTTAGCAGCTAATCCAAATTTTAGCCGTTATTCTCAAACATTACAAGAAATTGGCGATGGCTTCGTTATGGGTACTACTATTTTAAATAATCCAAAATTAATCAATAAACCAATCAAAGAATTAAAATTCTTTAACTTAGGAATTTCAGTTGTTTTAATTAAGAGAGGAACTGAAAGTATTTTACCTACTGGGGTAACTGTTTTACAAAAAAGTGATCTTATTACAATTCTAGGTAAGGTTGAAGATGTAACCAATGCTTTTGGTTTCTTAAATGTCGAAGACTAG", "aa": "MPRKRKNEDICVIGTGRFGSVVVSQLAKMNCPLLLIDKDEEVLKSYADLAQKVVIADAGDMKALRALNLQEIGTVVVAVPDNIEIVAALLELKVNNIIARATSKRHARVLKQIGVNVIVRSEYEAGVRTALLAANPNFSRYSQTLQEIGDGFVMGTTILNNPKLINKPIKELKFFNLGISVVLIKRGTESILPTGVTVLQKSDLITILGKVEDVTNAFGFLNVED", "aa_hexdigest": "1a6fafe44c35e545873eb27a2af4c245", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3310B", "ncbi_nrp_id": "WP_013354664.1", "uniref100_id": "UniRef100_UPI0001E3310B", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3310B", "RefSeq:WP_013354664.1", "UniRef:UniRef100_UPI0001E3310B" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E3310B", "uniref90_id": "UniRef90_C4XEZ5" }, "psc": { "uniref90_id": "UniRef90_C4XEZ5", "product": "Potassium uptake protein A", "uniref50_id": "UniRef50_C4XEZ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ5" ], "product": "Potassium uptake protein A" }, "genes": [], "id": "IGIGPGHMMC_910", "locus": "GCA000148625_00883" }, { "type": "cds", "sequence": "contig_1", "start": 503197, "stop": 503406, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGA", "aa": "MFGQAIIAILIFLGGLGIFALRIFLINLIFFKRRNSLSELEVVSHERGSGDSGQTKKLIMDSIGTLLII", "aa_hexdigest": "72e2093f0a50a4cc2a10b6822ff0e2da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7607.031999999997, "isoelectric_point": 9.687744712829591 }, "id": "IGIGPGHMMC_911", "locus": "GCA000148625_00884" }, { "type": "cds", "sequence": "contig_1", "start": 503596, "stop": 503739, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGA", "aa": "MPYYHNIGLQIIFLTLLIIGGLGYPVIHDILNFFRFLIKYKGKRRYQ", "aa_hexdigest": "b42de4efa5623b2c760ac6ac8621978d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.7618999999995, "isoelectric_point": 9.993905067443851 }, "id": "IGIGPGHMMC_912", "locus": "GCA000148625_00885" }, { "type": "cds", "sequence": "contig_1", "start": 503992, "stop": 504402, "strand": "+", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6", "UniRef:UniRef90_UPI0021AD00B6" ], "nt": "ATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGA", "aa": "MFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILV", "aa_hexdigest": "83415cf343d9ab6d60be54f0641ea146", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 503941, "stop": 504465, "observations": { "mutation": [ 504400 ], "stop": [ 504400 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021AD00B6", "query_cov": 1.0, "subject_cov": 0.7816091954022989, "identity": 1.0, "score": 256.0, "evalue": 7.61e-86, "gene_start": 1, "gene_end": 136, "reference_start": 18, "reference_end": 153, "reference_sequence": "MNFSKLTSTSLIICSILMFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILVWGKKKNYSNKYEYISEPVAIG" }, "paralog": false, "identity": 0.9885057471264368, "score": 332.0, "evalue": 7.5e-118, "description": "Internal stop codon at 504400. Nonsense mutation around 504400.", "psc": { "uniref90_id": "UniRef90_UPI0021AD00B6", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021AD00B6", "UniRef:UniRef50_UPI0021AD00B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6" ], "product": "potassium transporter TrkG" } }, "id": "IGIGPGHMMC_913", "locus": "GCA000148625_00886" }, { "type": "cds", "sequence": "contig_1", "start": 504966, "stop": 505751, "strand": "+", "frame": 3, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00", "UniRef:UniRef90_C4XF00" ], "nt": "ATGAAATTAAATTTATTTGCGTCTGAATACAAATCATTGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGA", "aa": "MKLNLFASEYKSLTNAEQKIIDYINENPNEFAENSIEKNAVETFASTAVLSRIYKKLGFSSYKDMQFYVKYNYLSNLKIPKELLDNSIYKIAKTYMEAINLTSKYLDIKEIDYICEEINKSKLVYCFGIGSSSISAKELSLNIEKFDYYSVFHNDFHNFLISLYSHELKENKPPVILFSKSGKTNEILFLCETLLEHKFPFLLITANRSLKNKYPHVLLHETIEQKERYHALSSKIVQQYIADIIADHLISNSPNKLNRKN", "aa_hexdigest": "49c50d289a29db5aba707230865bf656", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF00", "query_cov": 1.0, "subject_cov": 0.9456521739130435, "identity": 1.0, "score": 508.0, "evalue": 1.93e-181, "valid": true, "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XF00" }, "pscc": { "uniref50_id": "UniRef50_C4XF00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00" ], "product": "RpiR family transcriptional regulator" }, "genes": [ "rpiR" ], "id": "IGIGPGHMMC_914", "locus": "GCA000148625_00887" }, { "type": "cds", "sequence": "contig_1", "start": 505826, "stop": 506464, "strand": "-", "frame": 1, "gene": null, "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097SSE0", "UniRef:UniRef90_A0AB32XC18" ], "nt": "ATGTATGGTCCTGGTGTAATCGAAAGAATGGTTAAGTCTGTTTTATTAGGTGGTGCTCAAGGCATAAGAATTAGCCAAAGAGAAAATATTGAACCAGTCTTAAGAATAGTACCTAAAGACATTCCTGTTATTGGTATTACTAAAAAAATGTCTAAAGATTCTGAAGTATTTATTACTCCTGATTTAGATGATTTAAAATTCTTAATTGAAAGTAAATGTCCAATAATTGCAATAGATGCAACTTTAAGAAAAAGACCAAAAGAAAGTTTGGAAGACTTAGTTCAATATTTCAAGAAAAATAATCATAATCAGTTATTAATGGCAGATTGCTCTTCAATTGAAGATGTTGAAAATGCTTTTAAATTAGATTTTGATATTGTTTCAACTACTCTTAGAGGCTACACAAAAGACACATTAAATAAACCTAATAGTTACAATGATTTTGAATTTCTAAAAGAAGTAGTTGAATTAAATAAAAAGTACAAAAAATACATAATTGCTGAAGGCGGATTTAATACACCTGAAATGGTCCAAAAAGCCTATAAAATAGGTGCAAATGGTGTAGTTGTTGGCTCAGCAATTACTAGACCAACTTTTATAACAAAACAATTTGTTGACTATTTAAGCAATAAAAAATAA", "aa": "MYGPGVIERMVKSVLLGGAQGIRISQRENIEPVLRIVPKDIPVIGITKKMSKDSEVFITPDLDDLKFLIESKCPIIAIDATLRKRPKESLEDLVQYFKKNNHNQLLMADCSSIEDVENAFKLDFDIVSTTLRGYTKDTLNKPNSYNDFEFLKEVVELNKKYKKYIIAEGGFNTPEMVQKAYKIGANGVVVGSAITRPTFITKQFVDYLSNKK", "aa_hexdigest": "88085a39588a9e1300b2ce51633c86bd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0AB32XC18", "query_cov": 1.0, "subject_cov": 0.9137931034482759, "identity": 1.0, "score": 417.0, "evalue": 3.54e-147, "valid": true, "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "uniref50_id": "UniRef50_A0A097SSE0" }, "pscc": { "uniref50_id": "UniRef50_A0A097SSE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097SSE0" ], "product": "Putative N-acetylmannosamine-6-phosphate 2-epimerase" }, "genes": [], "id": "IGIGPGHMMC_915", "locus": "GCA000148625_00888" }, { "type": "cds", "sequence": "contig_1", "start": 507048, "stop": 507251, "strand": "-", "frame": 3, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "nt": "ATGGGTATTGGTTTAGCTATGATTCTTTTAGCTGAAAAAGAAAATCGTAAAAATGTAGCGATAACAATAGGTTCAGCAATGGGTGTTGCATTCTTAACAGGTATTACAGAACCTCTTGAATTTACATTCTTGTTTATTGCTCCTCAACTTTATTATGCTATTTATGTACCACTTAGTGGTTTAGCATATGCATTAATGGAATGA", "aa": "MGIGLAMILLAEKENRKNVAITIGSAMGVAFLTGITEPLEFTFLFIAPQLYYAIYVPLSGLAYALME", "aa_hexdigest": "27feea9e1a1b1384811f86f00fb7d837", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00168119FF", "query_cov": 0.9253731343283582, "subject_cov": 0.8611111111111112, "identity": 0.5, "score": 50.8, "evalue": 6.31e-07, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI00168119FF" }, "pscc": { "uniref50_id": "UniRef50_UPI00168119FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "IGIGPGHMMC_916", "locus": "GCA000148625_00889" }, { "type": "cds", "sequence": "contig_1", "start": 507378, "stop": 507632, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGATCACCAATAGGTTTAGATGGATTTATTTATGGTATATTAGGCCGTGCTTTAATGCCTTTTGGTTTACACCACATTCCAATTGTTTTAGCATATCAAACTGAATTTGGTGGTGTGTTATCTAAGGAAATGATTGATGCGGCTGTAACAGCTGGTAAAATTTCTTCAAAAGTAGCTACTGATTTAAAAAATTCATTAGAGAGTTTTTCTAAAGGAAAAGATGTTATTACTGGTGACCAAAACATCTGA", "aa": "MQRSPIGLDGFIYGILGRALMPFGLHHIPIVLAYQTEFGGVLSKEMIDAAVTAGKISSKVATDLKNSLESFSKGKDVITGDQNI", "aa_hexdigest": "a473ba75fcc88d38b11e284fafc49bdf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8979.317399999998, "isoelectric_point": 6.709013175964355 }, "id": "IGIGPGHMMC_917", "locus": "GCA000148625_00890" }, { "type": "cds", "sequence": "contig_1", "start": 507672, "stop": 507857, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTAAATACAAGTATTTTTGGTGGTATGGTTATTGGAATTGTCACTGCATATACATTTAATTATTTCTCTAAAATTGAATTACCACCTGCACTTTCATTCTTCTCAGGAATTAGATTAGTGCCAATTATTTTAGTACCTATTTCTTTTGGTGTATCTATTGTATTCTTATTATTATGA", "aa": "MKSLNTSIFGGMVIGIVTAYTFNYFSKIELPPALSFFSGIRLVPIILVPISFGVSIVFLLL", "aa_hexdigest": "3096ce78a6fd002e495bc430cc4f19ad", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6654.0108999999975, "isoelectric_point": 9.522705650329591 }, "id": "IGIGPGHMMC_918", "locus": "GCA000148625_00891" }, { "type": "cds", "sequence": "contig_1", "start": 507900, "stop": 508193, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCTATTGCAATTTTACCTATTGCTGGTTTATTATTAGGAATCGGTGGAGCTATTGGTGCAAATACAACTAATAGTGCTGGTCAAACAACTGCTAATATTTTCAAAGGTGTATCTGAAGTTATTTTTGGAAACTTACCTATTTTATTCTGTATTGCCATAGCGATCACATTTTCAAAAGACAAAGGTGGAGCAGGATTCTCGGCTGCTATTGCTTACCTTGTCTTTACTAGTTCTCAATTAGCATTCATCCAATTTGATGGTGTTGATATTGTTAAAAGTATTTTTTGA", "aa": "MLPIAILPIAGLLLGIGGAIGANTTNSAGQTTANIFKGVSEVIFGNLPILFCIAIAITFSKDKGGAGFSAAIAYLVFTSSQLAFIQFDGVDIVKSIF", "aa_hexdigest": "cae89d5cef978e68e85658c77fc48a9f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9895.530799999991, "isoelectric_point": 5.916791343688964 }, "id": "IGIGPGHMMC_919", "locus": "GCA000148625_00892" }, { "type": "cds", "sequence": "contig_1", "start": 508524, "stop": 509270, "strand": "+", "frame": 3, "gene": "cutC", "product": "PF03932 family protein CutC", "db_xrefs": [ "KEGG:K06201", "RefSeq:WP_013354670.1", "SO:0001217", "UniParc:UPI0001E33111", "UniRef:UniRef100_UPI0001E33111", "UniRef:UniRef50_A0A3P8LIN9", "UniRef:UniRef90_C4XF03" ], "nt": "ATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGATTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGATCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAAACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATATTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAA", "aa": "MKNIVTEVAVDSYQSALNADLAKAKRVELCSDLVLGGLTPSIGLYNLVRKNTKLDIVVMIRPRSGDFCYSASEIEHIIEDIKVFSSLKADALVVGILNGDFSINLEAMKKVIKAAKNTPIVFHRAFDVVNDQFKELENLKKLGVKRILTSGAKPNCDLGLERIKELVKVANGEITIMPGAGVNANNADKILQETKATEIHFTAKSIVNTSSLDKSKEVNYSSKELNEHLKIESEVAKITEIIDKIKAA", "aa_hexdigest": "c5864e3f813b635c3eec7b4ae5ea5634", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E33111", "ncbi_nrp_id": "WP_013354670.1", "uniref100_id": "UniRef100_UPI0001E33111", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33111", "RefSeq:WP_013354670.1", "UniRef:UniRef100_UPI0001E33111" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33111", "uniref90_id": "UniRef90_C4XF03" }, "psc": { "uniref90_id": "UniRef90_C4XF03", "product": "PF03932 family protein CutC", "uniref50_id": "UniRef50_A0A3P8LIN9", "kegg_orthology_id": "K06201" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LIN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LIN9" ], "product": "PF03932 family protein CutC" }, "genes": [ "cutC" ], "id": "IGIGPGHMMC_920", "locus": "GCA000148625_00893" }, { "type": "cds", "sequence": "contig_1", "start": 509502, "stop": 509621, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGA", "aa": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASM", "aa_hexdigest": "acea58cda84d9cc5bb1a24e6c4432506", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4389.0560000000005, "isoelectric_point": 9.10114688873291 }, "id": "IGIGPGHMMC_921", "locus": "GCA000148625_00894" }, { "type": "cds", "sequence": "contig_1", "start": 509631, "stop": 509849, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAACAAATAGCTACTATGTTACTGAAAGATGA", "aa": "MKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRTNSYYVTER", "aa_hexdigest": "b611d7471814514d97d9bc53d3a25979", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8499.762499999997, "isoelectric_point": 10.163263511657714 }, "id": "IGIGPGHMMC_922", "locus": "GCA000148625_00895" }, { "type": "cds", "sequence": "contig_1", "start": 509901, "stop": 510494, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_A5IY48", "UniRef:UniRef90_C4XF04" ], "nt": "ATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAG", "aa": "MEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK", "aa_hexdigest": "87e883a310d404affcc9da91bef69281", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 509601, "stop": 510494, "observations": { "mutation": [ 509619, 509847 ], "stop": [ 509619, 509847 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF04", "query_cov": 1.0, "subject_cov": 0.5969696969696969, "identity": 1.0, "score": 324.0, "evalue": 2.85e-109, "gene_start": 1, "gene_end": 197, "reference_start": 134, "reference_end": 330, "reference_sequence": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASMWNPRMKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRINSYYVTERWLGGTLTNSSTIFKRVKTMEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK" }, "paralog": false, "identity": 0.98989898989899, "score": 524.0, "evalue": 2.58e-190, "description": "Internal stop codon at 509619, 509847. Nonsense mutation around 509619, 509847.", "psc": { "uniref90_id": "UniRef90_C4XF04", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_A5IY48", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF04", "UniRef:UniRef50_A5IY48", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY48" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "IGIGPGHMMC_923", "locus": "GCA000148625_00896" }, { "type": "cds", "sequence": "contig_1", "start": 510503, "stop": 510592, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGA", "aa": "MDKLGLIKELRERTNGGMVDCKKALEESN", "aa_hexdigest": "3f533d98b1c752413aa9c3129e34b77a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3278.778800000001, "isoelectric_point": 6.088445091247558 }, "id": "IGIGPGHMMC_924", "locus": "GCA000148625_00897" }, { "type": "cds", "sequence": "contig_1", "start": 510713, "stop": 511375, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_A5IY49", "UniRef:UniRef90_C4XF05" ], "nt": "ATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAACTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAA", "aa": "MVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVETKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK", "aa_hexdigest": "eda6b40ea6038f55d9529abcfd054e65", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "5-prime", "pseudogene": { "start": 510491, "stop": 511375, "observations": { "mutation": [ 510590, 510614 ], "stop": [ 510590, 510614 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF05", "query_cov": 1.0, "subject_cov": 0.7482993197278912, "identity": 0.995, "score": 415.0, "evalue": 3.69e-145, "gene_start": 1, "gene_end": 220, "reference_start": 75, "reference_end": 294, "reference_sequence": "MGVTMDKLGLIKELRERTNGGMVDCKKALEESNWDIEKAITWLKSNGKIKAAKKANRVSAEGLVAIAANATKAVMVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVEAKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK" }, "paralog": false, "identity": 0.9863945578231292, "score": 554.0, "evalue": 1.58e-202, "description": "Internal stop codon at 510590, 510614. Nonsense mutation around 510590, 510614.", "psc": { "uniref90_id": "UniRef90_C4XF05", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_A5IY49", "go_ids": [ "GO:0003746", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF05", "UniRef:UniRef50_A5IY49", "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY49" ], "product": "Elongation factor Ts" } }, "id": "IGIGPGHMMC_925", "locus": "GCA000148625_00898" }, { "type": "cds", "sequence": "contig_1", "start": 511837, "stop": 512136, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06", "UniRef:UniRef90_C4XF06" ], "nt": "ATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAA", "aa": "MENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF", "aa_hexdigest": "76153966046da841818a9a276aaf90d7", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 511606, "stop": 512136, "observations": { "mutation": [ 511777 ], "stop": [ 511777 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF06", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 1.0, "score": 199.0, "evalue": 6.23e-64, "gene_start": 1, "gene_end": 99, "reference_start": 78, "reference_end": 176, "reference_sequence": "MMETKITTSNEQLPILKPILLASDFVEKIKGDKLYSDVMMLIAQDEDYVYFVSCLKEWEKNYELEPRFIKIYEEEGIMENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF" }, "paralog": false, "identity": 0.9829545454545454, "score": 344.0, "evalue": 2.8e-123, "description": "Internal stop codon at 511777. Nonsense mutation around 511777.", "psc": { "uniref90_id": "UniRef90_C4XF06", "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF06", "UniRef:UniRef50_C4XF06" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11499.206599999983, "isoelectric_point": 9.413754081726076 }, "id": "IGIGPGHMMC_926", "locus": "GCA000148625_00899" }, { "type": "cds", "sequence": "contig_1", "start": 512231, "stop": 512350, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTATTTTAGCTTTAGATAATACTTTGGATAATAAAATTATGAAAGCCAAAAATGCTGATGAAATTTATGAAGTAATGAGTAAATTTAATGTTGATATGCACCAATATGAATAA", "aa": "MISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE", "aa_hexdigest": "969d29bc32388b7cc542439509c441fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4577.236600000001, "isoelectric_point": 4.780352592468262 }, "id": "IGIGPGHMMC_927", "locus": "GCA000148625_00900" }, { "type": "cds", "sequence": "contig_1", "start": 512438, "stop": 512710, "strand": "-", "frame": 1, "gene": null, "product": "PTS EIIA type-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07", "UniRef:UniRef90_C4XF07" ], "nt": "ATGAAATTCAATAATTTTTTAAACAAAAATTCTATTTTTGTACATTCAAATTTTGATAAAAAAGATGATGTTTTGCACTTTTTATCACATCAACTAAATGAACAAAACTTTGTTAGTGATGAAAAGAAAATATATGAAGCTTTTTTATATAGAGAAGAATTAATGAATACTGCAATAGGCGGTGGCATTGCAATTTGTCAAGCATTAAGCTCAACAGTTAAAAAAGACACATTATGTATTTTAACTATGAATAATGATGGGGTTAATGATTGA", "aa": "MKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVND", "aa_hexdigest": "00845ba209b2df49145b0c4e31cd8a4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 512231, "stop": 512752, "observations": { "mutation": [ 512440 ], "stop": [ 512440 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF07", "query_cov": 1.0, "subject_cov": 0.5202312138728323, "identity": 1.0, "score": 181.0, "evalue": 7.9e-57, "gene_start": 1, "gene_end": 90, "reference_start": 15, "reference_end": 104, "reference_sequence": "MRKKRNLIIERTTKMKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVNDWKADDEKNVNLVFALVLSKNNRNLQIDTLQMISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE" }, "paralog": false, "identity": 0.9884393063583815, "score": 339.0, "evalue": 1.71e-121, "description": "Internal stop codon at 512440. Nonsense mutation around 512440.", "psc": { "uniref90_id": "UniRef90_C4XF07", "product": "PTS EIIA type-2 domain-containing protein", "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF07", "UniRef:UniRef50_C4XF07" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07" ], "product": "PTS EIIA type-2 domain-containing protein" } }, "id": "IGIGPGHMMC_928", "locus": "GCA000148625_00901" }, { "type": "cds", "sequence": "contig_1", "start": 512899, "stop": 513144, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAAAACTTAATTATTTGATTGAGGGCGGTAGAGTTAATAATAGCTTTAAAAAATTCGTTTTAAAAACAATGTCTAAATTAAGAATTTATCCTTATGTTAAGTTTATTAAACAACATGTTTCAACAGCTGGAATAGCTCGTGGTGTTAAGGGTGCAATAATTCAAATATTTAAAAAATTAGGAGCTTTTGTTAAAGCAAAAGATCTCAAAACATTAACAGAAAACTTCAAAATTGACTGA", "aa": "MPIKLNYLIEGGRVNNSFKKFVLKTMSKLRIYPYVKFIKQHVSTAGIARGVKGAIIQIFKKLGAFVKAKDLKTLTENFKID", "aa_hexdigest": "4667e27c91505db3ca2c179c5788b992", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9217.032699999992, "isoelectric_point": 10.371818733215331 }, "id": "IGIGPGHMMC_929", "locus": "GCA000148625_00902" }, { "type": "cds", "sequence": "contig_1", "start": 513265, "stop": 513612, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGGTTTTATTGTTGACGGCTTTATTAGTGAACCGCAAGAACTAATTCTTAAAAAAGGTTATGGTTATTTTCCTTTTTTAACAGATATTGATGGTGTAGTTTATAAAGATGGTATTGACTTAGACCAAGAAGATTTATTGCATAAACTTGATCAAGGAAAATCAGTTAAATCATCTATGCCAAATGTTAAAGCTGTTTTAGATACAGTGGACAAAATGACTAAAGAATATGATGAAGTAATTTACTTCCCAATTGGTTCATCATTATCTTCAAGTTGCAACAACATTGCAACAATTTGCAAAGAATATAAAAACTTTCACGTTTATGATAACTGCTGA", "aa": "MKKIGFIVDGFISEPQELILKKGYGYFPFLTDIDGVVYKDGIDLDQEDLLHKLDQGKSVKSSMPNVKAVLDTVDKMTKEYDEVIYFPIGSSLSSSCNNIATICKEYKNFHVYDNC", "aa_hexdigest": "b035d9d7828fa00fd8885e999c2c6109", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.75079999999, "isoelectric_point": 4.889824485778808 }, "id": "IGIGPGHMMC_930", "locus": "GCA000148625_00903" }, { "type": "cds", "sequence": "contig_1", "start": 513805, "stop": 514230, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATTGTGAAGACGATGAAAACATTATTTTGGAAACAAATGAATATGACTTAATTAATTTAAATACATATGATCGACCAAAAACAACTTTATTAATTAAACCATTACAAAACGGATTTAATTATCGATTAATTAACAATGCTTTTAGTAATTTGAAAGAAGGCATTAACTTAGGTTTATATATCGAAAAGATAAATGACCGTTTAACTAACTATGTTAATATCGGAAATATCAAGGTTTTTGTTATATCGGAAAAGATTTGCAAATACATTGAAAAAGATCAAATTTTATACATAAAACTTTTAAACTTCGTTAAAACAATTAGCACTATTAAAAATCATGTTATTATTTATCAAAACTTACCAGAAAGCTTAGATGAACTTATGGTAAAAACATTAGATGTACAATATGTTTGCAAATAG", "aa": "MENCEDDENIILETNEYDLINLNTYDRPKTTLLIKPLQNGFNYRLINNAFSNLKEGINLGLYIEKINDRLTNYVNIGNIKVFVISEKICKYIEKDQILYIKLLNFVKTISTIKNHVIIYQNLPESLDELMVKTLDVQYVCK", "aa_hexdigest": "3016bcd114f429d35f45dd01cd1a4cce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16553.991399999988, "isoelectric_point": 5.211192131042479 }, "id": "IGIGPGHMMC_931", "locus": "GCA000148625_00904" }, { "type": "cds", "sequence": "contig_1", "start": 514270, "stop": 514434, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGCTTGATTATGAAAAGAAAAATGAATTGCAACAATATATAAAAGAAAACATTCCAATTATTTCATATAAAACTAAACGTCAAAGCAAAGTTGCAATTTTAAAAAATAGAGTCATAGGTTACTCTGACCAAGATTCAGAATTCTTTAATAATGTTTCGTGA", "aa": "MMLDYEKKNELQQYIKENIPIISYKTKRQSKVAILKNRVIGYSDQDSEFFNNVS", "aa_hexdigest": "799899e9f7c932051b0b04333a124d22", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6459.319400000002, "isoelectric_point": 9.003799629211429 }, "id": "IGIGPGHMMC_932", "locus": "GCA000148625_00905" }, { "type": "cds", "sequence": "contig_1", "start": 514483, "stop": 514851, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTTATGCTTTGGGCATCAATGAATTTCTCTACAATAATGTCATTAGTAAATCAGACATTGAAGATATTTTGAGAATATTTGAGCTTAATAATATGTTTGGATATTACTACGTTAAAAACGGATTAATATTATTAATCTTTGAAACCAATTCGTCATTCTGGCTAAACCACTACACTAAAAAAGCACTTAAAAGATTAAAATTCTTACATGAAAGAAAAATTATTAATCCATTTTTCAATAGCTTTGCTTTTTTAAGTGTTAACACTTTCAAAAGTGTTGATGATGTTCAAGAATTAGTTGATAAATCAAAATACTTAATACAACACTTAAATAACAAACGCAAATTTGATAAATATTCTTCTTGA", "aa": "MAYALGINEFLYNNVISKSDIEDILRIFELNNMFGYYYVKNGLILLIFETNSSFWLNHYTKKALKRLKFLHERKIINPFFNSFAFLSVNTFKSVDDVQELVDKSKYLIQHLNNKRKFDKYSS", "aa_hexdigest": "9ce520f26f5d313bc2eb115ff611fbaf", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14595.680999999973, "isoelectric_point": 9.371076011657713 }, "id": "IGIGPGHMMC_933", "locus": "GCA000148625_00906" }, { "type": "cds", "sequence": "contig_1", "start": 514945, "stop": 515421, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA", "UniRef:UniRef90_UPI000FF46DBA" ], "nt": "ATGGATCAATTATTATTGGGAATAGCAATTATAATAGCATGTGTTATTTTAATAGACATCTTTATTATAGTTTTTATTTACTTAAATCATGTTTATAAAGTCAAAACAAATGGTTTAGCAATCTTTAAATTAGACTTTGGACAAAAAAAAATAATTCGCTTGAGTTCTAAAAGCTTAAGTGGATCTTTAAACATGGACTCACGTCTTCAAGGTCTTTCAGCTAATCAATATGTCGACTTTGATGTCTTTCTTTCTTACTTAGATGAAGAGAGCCAAAAAAACTTGACTGAATATCTTTCACAAAACGACAATAATGATAAAAGATTTAATATTTACTGCAAGATGAGTTCTAAAGGCTATCGAACACATCAAAATGCTCAAGGTTTAAAACTTCTAAACATTAAAATGACTAAAGAAAAAATTCTTTTAAAATTCTTCCCTCTTGGAAATAATCGATACTACTTAACAATCCACTGA", "aa": "MDQLLLGIAIIIACVILIDIFIIVFIYLNHVYKVKTNGLAIFKLDFGQKKIIRLSSKSLSGSLNMDSRLQGLSANQYVDFDVFLSYLDEESQKNLTEYLSQNDNNDKRFNIYCKMSSKGYRTHQNAQGLKLLNIKMTKEKILLKFFPLGNNRYYLTIH", "aa_hexdigest": "31f587c4c3e322c72fe1eeebbcfe6b84", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF46DBA", "query_cov": 1.0, "subject_cov": 0.8540540540540541, "identity": 1.0, "score": 300.0, "evalue": 2.32e-102, "valid": true, "uniref50_id": "UniRef50_UPI000FF46DBA" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF46DBA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18298.201200000007, "isoelectric_point": 9.373590278625485 }, "id": "IGIGPGHMMC_934", "locus": "GCA000148625_00907" }, { "type": "cds", "sequence": "contig_1", "start": 515465, "stop": 515968, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "ATGACAGTCCACTCTGCTAAAGGATTAGAATTTGACAATATCTTTTTAGTGGGAATGAGTGATCAAATTTTCCCTAACTATAAATCATTAAAATCAAACTCAGATGATGCTTTAGAAGAAGAAAGAAGACTTGCTTATGTGGCAATTACCAGAGCAAGAAATAGATTATTTATTAGCGATAGTCGTGGATATTTAATTGGTACAGATTTAGAAAAGAAACCATCACGTTTTATTCGTGAAATGGGAATTGAACTTAAAAACTTTATTCTTAAAACAGGTTCTGTTGGCCTTGATTTTGATGAAATAGTTGACGAAAAACAAATAAAAGCTTTGAACAAAAAAATTATAGTAGGTGACATTATTAGCCACACCTTCTTTGGCGAAGGCCAAGTTGTGGCTGTTGAAAACGATACAATTAGCGTTAGATTCACAGGACAAAAAACAATCAAAGTTTTAGCTAAAAATCACGCTTCAATTCGACTTTTAAAAAATACAGATGATTAA", "aa": "MTVHSAKGLEFDNIFLVGMSDQIFPNYKSLKSNSDDALEEERRLAYVAITRARNRLFISDSRGYLIGTDLEKKPSRFIREMGIELKNFILKTGSVGLDFDEIVDEKQIKALNKKIIVGDIISHTFFGEGQVVAVENDTISVRFTGQKTIKVLAKNHASIRLLKNTDD", "aa_hexdigest": "61feab745687eac4e2a0d90e0d4f1d63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 61, "aa_cov": 0.3652694610778443, "hmm_cov": 0.9672131147540983, "evalue": 1.527090363727141e-26, "score": 89.60643005371094, "start": 1, "stop": 61 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 23, "aa_cov": 0.1377245508982036, "hmm_cov": 1.0, "evalue": 5.6166396059103986e-11, "score": 38.21699523925781, "start": 36, "stop": 58 } ], "seq_stats": { "molecular_weight": 18852.393399999997, "isoelectric_point": 7.916862678527832 }, "id": "IGIGPGHMMC_935", "locus": "GCA000148625_00908" }, { "type": "cds", "sequence": "contig_1", "start": 516071, "stop": 516625, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "GTGGTCCAAAAAATTAATGAATTAAAGAAACAAAAAATTCAGCTTAAAAATATTGCTATTTTTTACCGTTCAAATTATTATTCGCGTCCTTTCGAAGAAGAATTAATTAATGAAAATATTAACCACAAGATTTTTAATGGTCAAAAATTCTTCCAAAGAACTGAAGTTAAGAATGCTCTAGCATTTTTAAGAGTTATCTATGATGGCTCTGATGTTTCATTAAATAGAATAATTAATGTTCCAAACCGTAAAATCGGAGAAGTAACTCTTAAAAAATTACAAGAAGCAGCAAATAATAAAAAATTAACTCTCTATTCATATTTAATTAAATATTACAAGGAATTGCCAGTACCTTTAAATGTTAAAAACAACATTGTTAAGTTTCTTAACAAAATCAACCTTTATCGTCAAGCTTTAAAAAGCAACTCAATTAGTGTTGTTTTAGATAAATTCTTAAAGGAAATTGGCTACTATGAACATATAGAAGAAGAAACCTCACTTCGTGGCACTGCTAAAGATAATGTTGCTGAATTAATTCGCTCAATAGTAACTTGA", "aa": "MVQKINELKKQKIQLKNIAIFYRSNYYSRPFEEELINENINHKIFNGQKFFQRTEVKNALAFLRVIYDGSDVSLNRIINVPNRKIGEVTLKKLQEAANNKKLTLYSYLIKYYKELPVPLNVKNNIVKFLNKINLYRQALKSNSISVVLDKFLKEIGYYEHIEEETSLRGTAKDNVAELIRSIVT", "aa_hexdigest": "7ac23def5b1dc56a662d7b9fe0e11521", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 109, "aa_cov": 0.5869565217391305, "hmm_cov": 1.0, "evalue": 1.5560251581368674e-23, "score": 79.71308898925781, "start": 2, "stop": 109 } ], "seq_stats": { "molecular_weight": 21618.849700000013, "isoelectric_point": 9.696447944641115 }, "id": "IGIGPGHMMC_936", "locus": "GCA000148625_00909" }, { "type": "cds", "sequence": "contig_1", "start": 516626, "stop": 516835, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "TTGATCCTTGATTTTGATAAGGATTTCAAGAATACTAAAACGATTATTTTAAATACAAATTACCGCTCAACTAAAACAATTTTAGATGCAGCTAACAAATTAATCAAACACAACAAAAAACGTTTTAGTAAAGATCTAATAACAAATCGTGAACAAGGAGCACCAATAGAATTTATTCATGCTTTTAGTGTCGAAGCTGAAGCTAGATGA", "aa": "MILDFDKDFKNTKTIILNTNYRSTKTILDAANKLIKHNKKRFSKDLITNREQGAPIEFIHAFSVEAEAR", "aa_hexdigest": "d2eec7a6214f6c4cf89776c21b7a5416", "start_type": "TTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 55, "aa_cov": 0.7971014492753623, "hmm_cov": 1.0, "evalue": 1.9361820197610067e-11, "score": 39.934757232666016, "start": 15, "stop": 69 } ], "seq_stats": { "molecular_weight": 8024.132399999999, "isoelectric_point": 9.62907848358154 }, "id": "IGIGPGHMMC_937", "locus": "GCA000148625_00910" }, { "type": "cds", "sequence": "contig_1", "start": 517190, "stop": 517636, "strand": "-", "frame": 1, "gene": null, "product": "UvrD-helicase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "nt": "ATGAGCAAACGAGATGAAATTTTGAATTCATTAAATGCTGAACAAAAAGAAGCTTTAATGTATTTTGATGGTCCTTTAAGAATCATCGCTGGAGCTGGAAGTGGTAAAACTAGAGTTTTAACTAGGAAGGTGGCTTATTTAATTAATGAACTTGGAATTAGCCCACGTTCAATTTTAGCAGTGACTTTCACTAATAAAGCTGCGAACGAAATGAGCGAACGTGTTAAACAATACGTCGGCGAAGAAGCTAATCAAATCGATATTTGTACTTTCCACTCTTTAAGCTCAAAGATTTTAAGGAAGGAAGCTATTCATGTTAATTTAAGTAATGACTTCCAAATTATTGATGAAACAGATAAGAAGACAATTATTGCTCAACTTTATAAAGCTGAAGGCATTTCAAATGAAGATGTTTCATACAAAAATGTTATGAAGATTTTCTCATGA", "aa": "MSKRDEILNSLNAEQKEALMYFDGPLRIIAGAGSGKTRVLTRKVAYLINELGISPRSILAVTFTNKAANEMSERVKQYVGEEANQIDICTFHSLSSKILRKEAIHVNLSNDFQIIDETDKKTIIAQLYKAEGISNEDVSYKNVMKIFS", "aa_hexdigest": "3ac20ae09ec7741bfea9f24769d48083", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002961EF37", "query_cov": 0.8513513513513513, "subject_cov": 0.9197080291970803, "identity": 0.508, "score": 139.0, "evalue": 6.76e-40, "valid": false, "product": "UvrD-helicase domain-containing protein", "uniref50_id": "UniRef50_UPI002204F9A8" }, "pscc": { "uniref50_id": "UniRef50_UPI002204F9A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "product": "UvrD-helicase domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_938", "locus": "GCA000148625_00911" }, { "type": "cds", "sequence": "contig_1", "start": 517649, "stop": 517807, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATGGCTTTAAGAACCAGACTTGGACTTTTTGATCGTTCAACAAACACCAGAGTAACATACTTAAAACATTACTCACAATCAAGCTATTTAGATGAAATCAAACTGTTACCATTCAAAAGTAATAATGAAGCTGTAATTTACATTAAAAAATAG", "aa": "MEMALRTRLGLFDRSTNTRVTYLKHYSQSSYLDEIKLLPFKSNNEAVIYIKK", "aa_hexdigest": "24259b18c6c6dda4e02fcec5cf01e20e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.107499999999, "isoelectric_point": 9.695158576965333 }, "id": "IGIGPGHMMC_939", "locus": "GCA000148625_00912" }, { "type": "cds", "sequence": "contig_1", "start": 517976, "stop": 518197, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCATAAAGGTGTTGTCAAGGCGGTAAACTTTTATTCTTTTATTATATTTTCAACAGTTATTTTACTTGTGCTATTTTTACTAATTCCATTGTTTTATAGTAGTAGAACAATTGGAATGCTGATTTTTAGAGTACAAATAATACCTGCTAAAAAGATGACTCCTTTAGCTTTAAAATTATTACCTTTAAGAAGAAATGCATTTGCTTTAATTTTCTGA", "aa": "MVHKGVVKAVNFYSFIIFSTVILLVLFLLIPLFYSSRTIGMLIFRVQIIPAKKMTPLALKLLPLRRNAFALIF", "aa_hexdigest": "ac321eef2cd15e3cbf404047094f3a7c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8367.376099999992, "isoelectric_point": 11.614382362365724 }, "id": "IGIGPGHMMC_940", "locus": "GCA000148625_00913" }, { "type": "cds", "sequence": "contig_1", "start": 518935, "stop": 519315, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCAATTATTTCACCAGGAATAAATAGCGCTAAAAGTTTAAAAAATATTTTAGAAGGTATGAAAGATCAAAATAATCAAGATTTTGAATTAATTTTAGCTTTAAGAAACCCTTCAGCTAAAATGTATTCTATTATTGAAAAATACTTAGCATTCTTCGGCACACGTTTAAAATTTATTACTAATAATCGCCAGAGAACTATACAAAGTGACATAGTTTGTGCTTTTCACTTAGTTAAAGGAAGCTATGCCACAATTATTAATAGTGACAACTCAATGCGTTCTTACTTTGCACAAGAATTAACAAACTATATTGAAAAATTTAACCCAGATGTTTTAGAATATCGTCCAAGATTAGTTGGCACAGTTAAATGA", "aa": "MKLSIISPGINSAKSLKNILEGMKDQNNQDFELILALRNPSAKMYSIIEKYLAFFGTRLKFITNNRQRTIQSDIVCAFHLVKGSYATIINSDNSMRSYFAQELTNYIEKFNPDVLEYRPRLVGTVK", "aa_hexdigest": "b318b768e8bb83ed6dfd8489bb0a2152", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 111, "aa_cov": 0.8809523809523809, "hmm_cov": 0.990990990990991, "evalue": 4.2700640074096984e-11, "score": 39.05664825439453, "start": 4, "stop": 114 } ], "seq_stats": { "molecular_weight": 14473.58709999998, "isoelectric_point": 9.551071739196775 }, "id": "IGIGPGHMMC_941", "locus": "GCA000148625_00914" }, { "type": "cds", "sequence": "contig_1", "start": 519325, "stop": 519459, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGCGTCGTTTTGGCGATAGTGGTTCAAGCGGTTTTGGAGTTGGTTTTGAAAGATTAGTAATGTATGTCACAGGTGTTGACAATATTAGAGATGTTATTCCATATCCAAGAACATCTGGAAATATTAAAATGTAA", "aa": "MRRFGDSGSSGFGVGFERLVMYVTGVDNIRDVIPYPRTSGNIKM", "aa_hexdigest": "3d7ca7e8900fcfe6bdb806abfc6963ef", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 0.9318181818181818, "subject_cov": 0.8541666666666666, "identity": 0.659, "score": 60.8, "evalue": 1.55e-11, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 36, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 6.050235583672184e-11, "score": 37.924766540527344, "start": 3, "stop": 38 } ], "seq_stats": { "molecular_weight": 4883.5452000000005, "isoelectric_point": 9.684585762023925 }, "id": "IGIGPGHMMC_942", "locus": "GCA000148625_00915" }, { "type": "cds", "sequence": "contig_1", "start": 519469, "stop": 519984, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGAGCCTGAAGTTGCTTTCTATGAATTAAAGGATGCAATCAAATTAGCTGATGACATGTTAAAATCTGTTATCAAAAATACAATTAAAAAACATCCTGCTGAATTTAAATTTTTAAATGAAACAGTTGACAAAGGTCTTTTAGAAAGATTAAATCACTTTTTAAAAACAAAACTTAGTGTTATTGACTATCGCGACGCAATTAAAGAATTGGCTAAAGTTAAAGACCAATTTGAAAATCAAGATATTGAATTCGGACTTGATTTAGCAACTGAACATGAAAGATATATTTCAGAAAAAATCTTTAATGGACCAGTAGCTGTAATTAATTTTCCAAAAAGTTTTAAAGCATTCTATATGCAACAAAATGATGACAATGAAACTGTAGCAGCTTTTGACTTATTAGTGCCAGGAATTGGCGAATTAATAGGTGGAAGCCAAAGAGAAGTTAGATATGACAAATTACTTCAAAGAGCTTTAGAAGTAGGAATCAGTCAAGAAGAATTGCAATGA", "aa": "MIEPEVAFYELKDAIKLADDMLKSVIKNTIKKHPAEFKFLNETVDKGLLERLNHFLKTKLSVIDYRDAIKELAKVKDQFENQDIEFGLDLATEHERYISEKIFNGPVAVINFPKSFKAFYMQQNDDNETVAAFDLLVPGIGELIGGSQREVRYDKLLQRALEVGISQEELQ", "aa_hexdigest": "27ce7c518c470dca834d2a40a8868e05", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 178, "aa_cov": 0.9883040935672515, "hmm_cov": 0.8089887640449438, "evalue": 2.9108913186867688e-21, "score": 71.81175231933594, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19666.273599999986, "isoelectric_point": 5.000831031799316 }, "id": "IGIGPGHMMC_943", "locus": "GCA000148625_00916" }, { "type": "cds", "sequence": "contig_1", "start": 519985, "stop": 520281, "strand": "-", "frame": 2, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "nt": "ATGTTAATAAGATCAACGTTAGCTAAAGAAGTTCATAAATATTTTGATGAACACGGTTACTTATACTTCAATGCTCCTATTATTACCAGCAATGATGGTGAAGGGGCTGGAGAAACTTTTGTAGTTAATGATGAAAATTCAAAAGATCCTTTCTTTGGGACTAGAAAAGCTACTTTAGGAGTTACGGGACAATTGCATGCTGAAAGTTATGCAATTGGATTTAAAAAAGTTTATACTTTTGCACCTACTTTTAGAGCAGAACACTCAAATACCAAAAAACATGCAGCAGAATTTTGA", "aa": "MLIRSTLAKEVHKYFDEHGYLYFNAPIITSNDGEGAGETFVVNDENSKDPFFGTRKATLGVTGQLHAESYAIGFKKVYTFAPTFRAEHSNTKKHAAEF", "aa_hexdigest": "8c25ed3d506a89953178343ac02a7092", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387B779D", "query_cov": 0.8775510204081632, "subject_cov": 0.8514851485148515, "identity": 0.6859999999999999, "score": 120.0, "evalue": 1.92e-33, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00387B779D" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B779D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "IGIGPGHMMC_944", "locus": "GCA000148625_00917" }, { "type": "cds", "sequence": "contig_1", "start": 520974, "stop": 521303, "strand": "+", "frame": 3, "gene": null, "product": "PQ-loop repeat-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35", "UniRef:UniRef90_UPI000FF44E35" ], "nt": "ATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGA", "aa": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLL", "aa_hexdigest": "b37ea1e873c200715c04adc101b217a4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 520974, "stop": 521513, "observations": { "mutation": [ 521301, 521388 ], "stop": [ 521301, 521388 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44E35", "query_cov": 1.0, "subject_cov": 0.5989010989010989, "identity": 1.0, "score": 205.0, "evalue": 5.96e-66, "gene_start": 1, "gene_end": 109, "reference_start": 1, "reference_end": 109, "reference_sequence": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLLWILALSGLVFFIVVYANKNARTNLGPDNGWMIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGKLY" }, "paralog": false, "identity": 0.9888268156424581, "score": 342.0, "evalue": 2.04e-122, "description": "Internal stop codon at 521301, 521388. Nonsense mutation around 521301, 521388.", "psc": { "uniref90_id": "UniRef90_UPI000FF44E35", "product": "PQ-loop repeat-containing protein", "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44E35", "UniRef:UniRef50_UPI000FF44E35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35" ], "product": "PQ-loop repeat-containing protein" } }, "id": "IGIGPGHMMC_945", "locus": "GCA000148625_00918" }, { "type": "cds", "sequence": "contig_1", "start": 521391, "stop": 521576, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGA", "aa": "MIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGIVLNALLCIYLGLVVPFNTAT", "aa_hexdigest": "ebb8bc4c1a04248b81aee1cce0420332", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 49, "aa_cov": 0.8032786885245902, "hmm_cov": 1.0, "evalue": 8.900704958914283e-09, "score": 31.071535110473633, "start": 3, "stop": 51 } ], "seq_stats": { "molecular_weight": 6462.937399999999, "isoelectric_point": 8.45897731781006 }, "id": "IGIGPGHMMC_946", "locus": "GCA000148625_00919" }, { "type": "cds", "sequence": "contig_1", "start": 521813, "stop": 522019, "strand": "+", "frame": 2, "gene": null, "product": "Aminopeptidase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7", "UniRef:UniRef90_A0A3P8MEW7" ], "nt": "ATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGA", "aa": "MQINKKEINNLYKKYLKNSNNKLIESAVTKNGIFNASYNNDMQTKHNFEFNTEVAKAGMTNQKSSGRC", "aa_hexdigest": "a730b0eb2dd06b5c996edae0430fcb1b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 521813, "stop": 522122, "observations": { "insertion": [ 522068 ], "mutation": [ 522017 ], "stop": [ 522017 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEW7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.8240000000000001, "score": 114.0, "evalue": 1.6e-31, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MKIDKKEINKFYKKYAKNSTNKLIESAMTKNGIFNASYNNDMQVKHNFEFNTQVTKTGITNQKSSGRCWIFSGLNMLKVKASKALMLKILNSAKTTFYFETN" }, "paralog": false, "identity": 0.803921568627451, "score": 164.0, "evalue": 8.45e-54, "description": "Internal stop codon at 522017. Frameshift due to insertion around 522068. Nonsense mutation around 522017.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEW7", "product": "Aminopeptidase E", "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEW7", "UniRef:UniRef50_A0A3P8MEW7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7" ], "product": "Aminopeptidase E" } }, "id": "IGIGPGHMMC_947", "locus": "GCA000148625_00920" }, { "type": "cds", "sequence": "contig_1", "start": 522284, "stop": 522754, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACATTTAATTCAAGCAATACTCATGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGTGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAAGCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAAGATAAAAAATTTGTCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAGCATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTACTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAATCACTTAATGTTACAATGGATGTAATTAAAAAGGCTGTTATTGCTTCATTAAAAGATAATGAACCTGTTTGA", "aa": "MPETFNSSNTHGLNTALNCQLISVTKEIREAIKNKASQEVLMKIKHEALERIFEINAKALGLPPQKFSFEYRDKDKKFVRIENVTPLEFLEKHVGTDFLNKVNLLDDPRKENPKNRLLVAKYYKSVIEEKGLESLNVTMDVIKKAVIASLKDNEPV", "aa_hexdigest": "e2aab629c3be16473af76dd772ac6ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17882.50329999999, "isoelectric_point": 8.87009220123291 }, "id": "IGIGPGHMMC_948", "locus": "GCA000148625_00921" }, { "type": "cds", "sequence": "contig_1", "start": 522830, "stop": 522952, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCCAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGA", "aa": "MLNIKNTLTKEDRVNYRLSVPTHAMTIVGVNLDKDNNPIN", "aa_hexdigest": "18274f622400f630a0444b91b01d687c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4553.182200000001, "isoelectric_point": 8.210580635070801 }, "id": "IGIGPGHMMC_949", "locus": "GCA000148625_00922" }, { "type": "cds", "sequence": "contig_1", "start": 523251, "stop": 523352, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGA", "aa": "MTAKVIKTIAQVLVVLSLLFICSLAIFNMQAGH", "aa_hexdigest": "5ee581c4bc7dae510991c297905e9406", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3574.4103999999998, "isoelectric_point": 9.305705070495605 }, "id": "IGIGPGHMMC_950", "locus": "GCA000148625_00923" }, { "type": "cds", "sequence": "contig_1", "start": 523641, "stop": 523784, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGA", "aa": "MPKRAKLITANIKPVTKPIKVSFQITLPYSPGSISFKAKARLTEILA", "aa_hexdigest": "e981b0f201ed9c55f48b7cd127fac8e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5168.235, "isoelectric_point": 10.79015407562256 }, "id": "IGIGPGHMMC_951", "locus": "GCA000148625_00924" }, { "type": "cds", "sequence": "contig_1", "start": 523979, "stop": 524572, "strand": "-", "frame": 1, "gene": "mgtE", "product": "magnesium transporter MgtE N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "nt": "ATGAAACTACTACAAGAATTACAAAGTGATGAACTAGCTGATGTTTTAGAAGAATTACCTTCGAATGTCACAAGCAAAATCTTAGCTTATACACCTCAAGAAAAAAGAGCTGAATTAAATAAACTTTTAAGCTATGAAGATGATGAAGTTGGAAGCATAATGTCAATAGACATTTCTTCAATTAGTAATTCATATACTTGCGAACAAGCTTTACATAAAATTAGACGTGATTATCATAAGAAAAACGCTGAATTAGTGCATTACTATTACGTAGTTAATGAAACAAATAAACTAATTGGTGTTTTAACACTAGAAGAAATAGTTTTTGCTGATCCTAATGCTAAAATTGATGATATTTATAATCCTGTAACTTATGTTTCAGCTCATGATAAAACTGAAGTAGCAGCTCAGGTTTTTAGTGAACACGATATGTCAGTATTGCCTGTAGTTAACCATGAAAAAAGATTAGTCGGAATGATTACAAGTGACGACGTTATTGATGTTATTAAAGAAGAAGCTACAGAAGACTTATACAAAATGGCAGGTATTAGTAGCCATGAAGTTAATGAAAGCGAATACTTAAAAACTCCATGA", "aa": "MKLLQELQSDELADVLEELPSNVTSKILAYTPQEKRAELNKLLSYEDDEVGSIMSIDISSISNSYTCEQALHKIRRDYHKKNAELVHYYYVVNETNKLIGVLTLEEIVFADPNAKIDDIYNPVTYVSAHDKTEVAAQVFSEHDMSVLPVVNHEKRLVGMITSDDVIDVIKEEATEDLYKMAGISSHEVNESEYLKTP", "aa_hexdigest": "79384559658f2603d67d230394329641", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C659EE", "query_cov": 0.9137055837563451, "subject_cov": 0.8411214953271028, "identity": 0.522, "score": 177.0, "evalue": 8.53e-53, "valid": false, "product": "magnesium transporter MgtE N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0024C659EE" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C659EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "product": "magnesium transporter MgtE N-terminal domain-containing protein" }, "genes": [ "mgtE" ], "id": "IGIGPGHMMC_952", "locus": "GCA000148625_00925" }, { "type": "cds", "sequence": "contig_1", "start": 524576, "stop": 524827, "strand": "-", "frame": 1, "gene": null, "product": "Magnesium transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "nt": "ATGAGCGAAAGTCAAGAGATTAGTTTGCAAAACGAACTACATGAAATTATTAAAACTAAAAATGTAACTAAAGCCAGAGAATTTATTGATAATAATCCAATGGCTGATGTTGCAAACTTATTAAATGAATTACAGTTAGAAGAACAGCTAACTTTTTTGCCTTTACTTAAAACTAGTGAAGCTGCTGAATTATTTAGCTACCTTGATGAAGAAGTCCAAGCTAATTTAGCTCAAAGTTTTACAGAAGATTGA", "aa": "MSESQEISLQNELHEIIKTKNVTKAREFIDNNPMADVANLLNELQLEEQLTFLPLLKTSEAAELFSYLDEEVQANLAQSFTED", "aa_hexdigest": "e79bd320d1434f37e735e5c9a4e1b022", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A3P8KNN3", "query_cov": 1.0, "subject_cov": 0.8645833333333334, "identity": 0.892, "score": 136.0, "evalue": 3.59e-40, "valid": false, "product": "Magnesium transporter", "uniref50_id": "UniRef50_A0A3P8KNN3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "product": "Magnesium transporter" }, "genes": [], "id": "IGIGPGHMMC_953", "locus": "GCA000148625_00926" }, { "type": "cds", "sequence": "contig_1", "start": 524829, "stop": 525410, "strand": "-", "frame": 3, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "RefSeq:WP_013354684.1", "SO:0001217", "UniParc:UPI0001A8C272", "UniRef:UniRef100_C4XF17", "UniRef:UniRef50_L0RXD2", "UniRef:UniRef90_C4XF17" ], "nt": "ATGTGTTTAGAATCTAAAATTGGTTTTTATGTAATTGTTGCATTATTAGTTACTTTAATTTTAATTTCAATAATGCCACTTATAGTTAATGCAATTAAAAACAAAATGGCTAGAAGTCATTTCCAAAAATTAGGTCAAAGTAGTCAAATTAGATTAATTAACCAACTACGTGAAGCTGTTGAATATTTATCAAAAAATAAAATTGGAGCTTTAATTACAATTGAAAACAATGATAATATTGACAATTTGAGAACTGATGGGGTAATTTTAAATGCTAATATCAGTAGTAGCTTACTTATTTCAATATTCAATAAATATAGTCCATTGCATGATGGAGCTGTAATTATAAGAGATAATAAAATTTATTATGCTTCTACTTTTTATAAAATCACCAGAAAATCAATTGATAATCAATATGGTGCTCGTCATAGAGCTGCTATGGGAATTAGTGAACTTTGTGATGCTACCACAATAATTGTTAGTGAAGAAACAGGGGATGTTAAATTTGTTAAAAATGGTACTTTCTACAACATTCGTCTTGAACAATTCCAAGAACAACTAGTTAAATACTTAAAGGATTAA", "aa": "MCLESKIGFYVIVALLVTLILISIMPLIVNAIKNKMARSHFQKLGQSSQIRLINQLREAVEYLSKNKIGALITIENNDNIDNLRTDGVILNANISSSLLISIFNKYSPLHDGAVIIRDNKIYYASTFYKITRKSIDNQYGARHRAAMGISELCDATTIIVSEETGDVKFVKNGTFYNIRLEQFQEQLVKYLKD", "aa_hexdigest": "fd947db9bc030b75f4722be4eed0c1e6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C272", "ncbi_nrp_id": "WP_013354684.1", "uniref100_id": "UniRef100_C4XF17", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C272", "RefSeq:WP_013354684.1", "UniRef:UniRef100_C4XF17" ] }, "ips": { "uniref100_id": "UniRef100_C4XF17", "uniref90_id": "UniRef90_C4XF17" }, "psc": { "uniref90_id": "UniRef90_C4XF17", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_L0RXD2", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_L0RXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXD2" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_954", "locus": "GCA000148625_00927" }, { "type": "cds", "sequence": "contig_1", "start": 525420, "stop": 525722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354685.1", "SO:0001217", "UniParc:UPI0001E32E79", "UniRef:UniRef100_UPI0001E32E79", "UniRef:UniRef50_C4XF18", "UniRef:UniRef90_C4XF18" ], "nt": "ATGGCAATTATTGATAGAGATCTAGCAACATGTGCTGAAAGAATTCAAAAAAGAAGAGAATTACTATTAAAACTTTATAATGAAAATCAAGCTGAATTAGCTAATCCAGCTTTGATTGAAGATGAAAAACCTATTACTGGTGAATGTCAGGAATTTATGTGTTTAGTAAATAAACACGAGAAAGAAGATCCTAAAGTTAGAAAGCAAAAAATTATTCTTAAAGTTTTAGGAGCCAGCTTATCAGTTTTAATTATTTTACTTTGCACACTACTAGCTGTTTTAATTTCAAGAGTTTATGCTTAG", "aa": "MAIIDRDLATCAERIQKRRELLLKLYNENQAELANPALIEDEKPITGECQEFMCLVNKHEKEDPKVRKQKIILKVLGASLSVLIILLCTLLAVLISRVYA", "aa_hexdigest": "4b839e65653b1d68ba0fad52ab90244f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32E79", "ncbi_nrp_id": "WP_013354685.1", "uniref100_id": "UniRef100_UPI0001E32E79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E79", "RefSeq:WP_013354685.1", "UniRef:UniRef100_UPI0001E32E79" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32E79", "uniref90_id": "UniRef90_C4XF18" }, "psc": { "uniref90_id": "UniRef90_C4XF18", "uniref50_id": "UniRef50_C4XF18" }, "pscc": { "uniref50_id": "UniRef50_C4XF18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF18" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.454199999987, "isoelectric_point": 7.639978981018066 }, "id": "IGIGPGHMMC_955", "locus": "GCA000148625_00928" }, { "type": "cds", "sequence": "contig_1", "start": 526338, "stop": 526502, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAGAGGAATTTTTCAAAGGCGTAAGTAGACCAGTAAATGAAAAATTACAAAAAATTTTTATGCAATTGGATCTAGTTGAACAAACTGGACATGGAGTGCCTTTAATTGTTAAAAAATACGGTGAAGAAGCATTTGAAATTGACGAAAATACAATTTGA", "aa": "MTKEEFFKGVSRPVNEKLQKIFMQLDLVEQTGHGVPLIVKKYGEEAFEIDENTI", "aa_hexdigest": "d94038f165e125f65d26b1db5f48d434", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6255.132399999999, "isoelectric_point": 4.972127342224121 }, "id": "IGIGPGHMMC_956", "locus": "GCA000148625_00929" }, { "type": "cds", "sequence": "contig_1", "start": 526608, "stop": 527297, "strand": "-", "frame": 3, "gene": null, "product": "Putative ATP-dependent DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7", "UniRef:UniRef90_Q6GYZ7" ], "nt": "ATGCTCAATAAAAGAGGAATGGGAACAGTTTATTTTGGCGTAAAAGATGATCGTACAGTTGTTGGTTTAGAAATTGGCAATAAAACCTTGAGAGATATTTCTCAAGCTATTTCAAACAATGTTAAGCCAGAAATAATGCCTAGAATTTCGATTGAATTTATTGATAACCAAAATGTAATTAAAGTTGAAGCTGAAGGAGAACAAAAACCTTATTCAGCTTATGATAAATATTATATTCGTATTGGAGATGAAGATAAAAAACTTAATAGAGATAAATTAGTAGATTTTGTGCAAAAATCAGTTTCTAAAATAAATATTTGTGAAATTGAAAACAAAGATCAAGATTTGACATTTAATAGTTTTTTGCTATATGCTAATATTAAAAATTTAACAGTAAATAAAGATACTTTCGAAAAAAAATTTGATTTAAAGACTAAAAATAATAAATACAATTTAATGTCTTATTTATTAGCAGATAATAATGATATTTCAATTAAAGTTAATACTTTTAAAGATAATGATAAAAGTGTTTTGTTGAAAAGAAATGAATATGGAAATAAATGTTTATTTTATGCAATTGAGAGTGTTTTAAATTATGTAGGTTCTATTAATGATACTTATGTAGATTTGTCAACTGCAACCAGGAGTGAACAAAAATTATTTGATTTTGAGTGTTTTAGAGAAGCATGA", "aa": "MLNKRGMGTVYFGVKDDRTVVGLEIGNKTLRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGDEDKKLNRDKLVDFVQKSVSKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKKFDLKTKNNKYNLMSYLLADNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESVLNYVGSINDTYVDLSTATRSEQKLFDFECFREA", "aa_hexdigest": "02b4235d0a7050e67d783e556b489f92", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 526305, "stop": 527384, "observations": { "mutation": [ 526340, 526583, 526610, 527369 ], "stop": [ 526340, 526583, 526610, 527369 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GYZ7", "query_cov": 1.0, "subject_cov": 0.5264367816091954, "identity": 0.9740000000000001, "score": 437.0, "evalue": 7.54e-152, "gene_start": 1, "gene_end": 229, "reference_start": 30, "reference_end": 258, "reference_sequence": "MNKMPWESEEVEYKKSTSELKEAIISLTAMLNKKGTGTVYFGVKDDRTVVGLEIGNKTMRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGEEDKKLNRDKLVDFVQKSVSKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKNFDLKTKNNKYNLMSYLLADNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESVLNYVGSINDTYVDLSTATRSEQKLFNFECFREAWLNACIHNRWDEKYPPQVNIFKDYIQIESNGGIPRNMTKEKFFKGVSRPVNEKLQKIFMQLDLVEQTGHGVPLIVKKYGEEAFEIDENTIWVKIPFNRKGFKRINEKENKNNLNDNQIKVIKTIEKESNITINQLSSTLSLSEGYIKKIINQLKNKNLIERNGLRKNGNWTIISYNL" }, "paralog": false, "identity": 0.9693593314763231, "score": 691.0, "evalue": 2.63e-254, "description": "Internal stop codon at 526340, 526583, 526610, 527369. Nonsense mutation around 526340, 526583, 526610, 527369.", "psc": { "uniref90_id": "UniRef90_Q6GYZ7", "product": "Putative ATP-dependent DNA helicase", "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GYZ7", "UniRef:UniRef50_Q6GYZ7" ] }, "pscc": { "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7" ], "product": "Putative ATP-dependent DNA helicase" } }, "id": "IGIGPGHMMC_957", "locus": "GCA000148625_00930" }, { "type": "cds", "sequence": "contig_1", "start": 527583, "stop": 527765, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTTTAAGTGCCTTTTTCGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAATATGAATGAAGTAAGTTTACTATTTTGA", "aa": "MLKIFKYMPARIKFFAFLSAFFAILQPFLSMLNPTITKQLITLIAKVQNGTNMNEVSLLF", "aa_hexdigest": "96dbece08c749803ab4bf2183c12e22f", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6915.382, "isoelectric_point": 10.294907951354979 }, "id": "IGIGPGHMMC_958", "locus": "GCA000148625_00931" }, { "type": "cds", "sequence": "contig_1", "start": 527796, "stop": 528071, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGGGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGA", "aa": "MIYLIIMAIGFAFLLMIVAFLSAFFAARTCIYGTYYLRKNLFEHLLTLSRSNLEKYSVGSLITRFSNDLNKIKDGIFVVVRGLVVSPFFVV", "aa_hexdigest": "d5b6c3db62fa9e0a7ff9d94b6f109c51", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10382.362399999989, "isoelectric_point": 9.727586174011229 }, "id": "IGIGPGHMMC_959", "locus": "GCA000148625_00932" }, { "type": "cds", "sequence": "contig_1", "start": 528381, "stop": 529370, "strand": "+", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXL5", "UniRef:UniRef90_Q6GYZ6" ], "nt": "GTGCTTTTTGGCAATGTAGCCATTTTTGCTCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTCACAGCAAATTTAAGCATGATTTCAATGGGTGTTTTCAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAATAACTAGTGGAGCTATTGAATTTAAGAATGTTAATCTTGCCTATCCTATCTATAAAAAAGGCGAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGTCAAAAAATTGGAATTATAGGCAAAACCGGAAGTGGTAAAAGTACACTTGTTAAACTTTTAACCCATGAATATTTACTAGATAAAAAAGATGGTGAAATCATTATTGACTCGCACAATATTAATGAAATAGATACTAATAACTTTTATGAAAAAGTTACTCAAGTTTATCAAAAACCAATGATTATTTCAGGCTCAATTAAAGAAAATATTACTTTCTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAAAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCATTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAACAAAACTTAAAAGATAGTACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAGATCAAATCTTTGTTCTTGATAAGGGACAAATTGCTGCTAGTGGTAAACACAACCAACTATTAAACACAAGTTCTATATATAAAGATATATGA", "aa": "MLFGNVAIFALVAYLIGSKGDDNVAKLIGDVSQFTANLSMISMGVFNTLFTINRLFRSSVSAKRYIELMNSKSEIPFVVSDKKITSGAIEFKNVNLAYPIYKKGELVDTKEALKNINLKIEAGQKIGIIGKTGSGKSTLVKLLTHEYLLDKKDGEIIIDSHNINEIDTNNFYEKVTQVYQKPMIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDDKYEAKVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKQNLKDSTLIVISQRINSIKDSDQIFVLDKGQIAASGKHNQLLNTSSIYKDI", "aa_hexdigest": "b23cc9edd62f13fdec34e1434da4a0a0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 528081, "stop": 529400, "observations": { "mutation": [ 528198, 528315, 528360, 529368 ], "stop": [ 528198, 528315, 528360, 529368 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GYZ6", "query_cov": 1.0, "subject_cov": 0.543801652892562, "identity": 0.9790000000000001, "score": 614.0, "evalue": 3.73e-217, "gene_start": 1, "gene_end": 329, "reference_start": 267, "reference_end": 595, "reference_sequence": "MLKIFKYMPARIKFFAFLSAFFAILQPFLSMLNPTITKQLITLIAKVQNGTSMNEVSLLFWNITTSGTSQSMIYLIIMAIGFAFLLMIIAFLSAFFAARTCIYGTYYLRKNLFEHLLTLSRSNLEKYSIGSLITRFSNDLNKIKDGIFVVVRGLVVSPFFVVWGLVFTTTTNVYLSIAIYIIIPFLVGAAVFAIFKLFPYYRKENWILDQLNELSKEDINAIALIKSYNLEEPRYEKYFNKNQEWFTIYRKNVVFGSFAWPIINGFVLFGNVAIFALVAYLIGSKGDDNVAKLIGDVSQFTANLSMISMGVFNTLFTINRLFRSSVSAKRYIELMNSKSEISFVVSDKKITSGAIEFKNVNFAYPIYKKGELVDTKEALKNINLKIEAGQKIGIIGKTGSGKSTLVKLLTHEYLLDKKDGEIIIDSHNINEIDTNNFYEKVTQVYQKPMIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDNKYDARVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKQNLKDSTLIVISQRINSIKDSDQIFVLDKGQIVASGKHNQLLNTSSIYKDIWQSQEQKGGE" }, "paralog": false, "identity": 0.9772209567198178, "score": 823.0, "evalue": 4.44e-302, "description": "Internal stop codon at 528198, 528315, 528360, 529368. Nonsense mutation around 528198, 528315, 528360, 529368.", "psc": { "uniref90_id": "UniRef90_Q6GYZ6", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_A0A3P8KXL5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GYZ6", "UniRef:UniRef50_A0A3P8KXL5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KXL5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXL5" ], "product": "ABC-type multidrug/protein/lipid transport system ATPase component" } }, "id": "IGIGPGHMMC_960", "locus": "GCA000148625_00933" }, { "type": "cds", "sequence": "contig_1", "start": 529568, "stop": 529867, "strand": "+", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23", "UniRef:UniRef90_C4XF23" ], "nt": "ATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATGAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAACTTAGTGCTCGCCTTGGTCGGAATTTAAGATTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGCTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCTTTAACTAATGATATTCAAAATATAACTGAAGCAATCTGA", "aa": "MGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFMKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAI", "aa_hexdigest": "bc4bcfd410fefb5195e7b439a7c380a5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 529367, "stop": 529963, "observations": { "mutation": [ 529394, 529505, 529562, 529865 ], "stop": [ 529394, 529505, 529562, 529865 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF23", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.99, "score": 184.0, "evalue": 2.17e-57, "gene_start": 1, "gene_end": 99, "reference_start": 68, "reference_end": 166, "reference_sequence": "MTISRTKSRWIMPRFSNLRFRQKGEKKISFKIFFKFLSNFQISKSVWIFTVLFGLLVASLTNIASWLMGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFIKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAIWDILISVISIIFEFSFITIMMFIYVPLLAAII" }, "paralog": false, "identity": 0.9646464646464646, "score": 357.0, "evalue": 2.87e-128, "description": "Internal stop codon at 529394, 529505, 529562, 529865. Nonsense mutation around 529394, 529505, 529562, 529865.", "psc": { "uniref90_id": "UniRef90_C4XF23", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF23", "UniRef:UniRef50_C4XF23" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "IGIGPGHMMC_961", "locus": "GCA000148625_00934" }, { "type": "cds", "sequence": "contig_1", "start": 529922, "stop": 530209, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTTTATTTATGCTCCACTTTTAGCAGCTATTATTTTAGTAATTGTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGA", "aa": "MMFIYAPLLAAIILVIVPINASVIFLIIRSNQKFYIQQQERLGEFNGYLEEIMDAFPLVNLHQQKENVAKEFDKYSRALIKPDASATTRMNIVFP", "aa_hexdigest": "aa4d0a1da3236a8abdd5c89708994cfb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10962.781599999988, "isoelectric_point": 6.554865837097168 }, "id": "IGIGPGHMMC_962", "locus": "GCA000148625_00935" }, { "type": "cds", "sequence": "contig_1", "start": 530303, "stop": 530719, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGA", "aa": "MSAGVVMSFTLYLFRISDNISLLLEVINGIQHGLGSIVRINKILALVPQFDEVKLNELDYKTGKIEFKNVSFAYASKPDKLVLKNISFTIEKGKSLALVGHTGCGKTTIAKLLSKFYVPTSGDILIDGQSILKTKSKS", "aa_hexdigest": "cfd665868848676fff88c05bfc11cde4", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 52, "aa_cov": 0.37681159420289856, "hmm_cov": 1.0, "evalue": 9.547647846484554e-17, "score": 57.910335540771484, "start": 83, "stop": 134 } ], "seq_stats": { "molecular_weight": 15094.607899999983, "isoelectric_point": 9.547654914855958 }, "id": "IGIGPGHMMC_963", "locus": "GCA000148625_00936" }, { "type": "cds", "sequence": "contig_1", "start": 530741, "stop": 531232, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGCAAGAAACTTATATTTTTAATGATACAGTCAAAAATAATTTAACTATTGTTAATCCAAATATAAAGGATAAAGACTTTAATGACATAGTAGAAAAAACTTCAGTAGATGAATTTATTAATTTAATGCCTGAAAAATTTGACACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGCCAAAAGCAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAACATTGATACAATAACTGAATTAAAAGTGCAACATGCAATGAAATATTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAATACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGATCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTGAATGATACAAAAATTGATTAG", "aa": "MQETYIFNDTVKNNLTIVNPNIKDKDFNDIVEKTSVDEFINLMPEKFDTVLKSNGANLSEGQKQLIAISRSLIASKPISILDEATSNIDTITELKVQHAMKYLMKDRSMLIIAHRLNTIKNVDEILVINEGEIIERGNHDQLMKQNGHYKKIYSLGLNDTKID", "aa_hexdigest": "cb2eedb1d93335b294576d5f6ebe5e95", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.48466257668711654, "hmm_cov": 0.8780487804878049, "evalue": 6.851423283729795e-10, "score": 35.70619201660156, "start": 8, "stop": 86 } ], "seq_stats": { "molecular_weight": 18590.0975, "isoelectric_point": 5.5888872146606445 }, "id": "IGIGPGHMMC_964", "locus": "GCA000148625_00937" }, { "type": "cds", "sequence": "contig_1", "start": 531269, "stop": 531985, "strand": "-", "frame": 1, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "KEGG:K07133", "RefSeq:WP_013354689.1", "SO:0001217", "UniParc:UPI0001E32E7D", "UniRef:UniRef100_UPI0001E32E7D", "UniRef:UniRef50_UPI0001E32E7D", "UniRef:UniRef90_UPI0001E32E7D" ], "nt": "ATGAAAGAAGCCGACACTAAAACTAATTATTTGGTAAATGAATTCAAAAGAACTTATTTAATAGATATAGTTGAAAAAAATAAAATAGAAGATATTGATAACTTAGATGACATAATCAATATTTTAGCTTCTAATATAGGTTCATACACTAATTCTTATAAATTATCATTAACTTTTAAATCAATTAAGAAAACAAATATTTCAGATGCAACAATTAGAAAATATATTGAAAATTTAAAAAATGCTTTCATAATTAATGAAGTCAAGAAATATGACATAAAAGGTAAAAAGTATATTGATGCTTCATACAAATATTATTTTGAAGATATAGCCTTAAGAAATGCAAAAATCAATTTTGCACAAAATGAAGAAAATCATTTAATGGAAAATATCATTTATAATGAATTGCTTTTAAGAGGTTACAATGTTAATGTTGGAATAGTTGAAATAAGACCTAATGCTAATCAGAAAGTTCAATTAGAAGTAGATTTTATTGCCCAAAAAGGTAATCAAAAAGTTTATATTCAATCTTATTTAAATATGTATGATCAAAATAAAGAAATTCAAGAAAAAAGACCTTTTAAAAACATCAATGATAATTTTAAAAAAATTATTATTATTAAAGATGACCCTATTTTTGATACCGATACTAATGGTTATAAAATTTATGGAATCAAACAATTTTTACTTGATTTAGATATTCTAAACAAAAATTAA", "aa": "MKEADTKTNYLVNEFKRTYLIDIVEKNKIEDIDNLDDIINILASNIGSYTNSYKLSLTFKSIKKTNISDATIRKYIENLKNAFIINEVKKYDIKGKKYIDASYKYYFEDIALRNAKINFAQNEENHLMENIIYNELLLRGYNVNVGIVEIRPNANQKVQLEVDFIAQKGNQKVYIQSYLNMYDQNKEIQEKRPFKNINDNFKKIIIIKDDPIFDTDTNGYKIYGIKQFLLDLDILNKN", "aa_hexdigest": "39f8fb759d1ed5c96db8e8e6e9725ae6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32E7D", "ncbi_nrp_id": "WP_013354689.1", "uniref100_id": "UniRef100_UPI0001E32E7D", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E7D", "RefSeq:WP_013354689.1", "UniRef:UniRef100_UPI0001E32E7D" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32E7D", "uniref90_id": "UniRef90_UPI0001E32E7D" }, "psc": { "uniref90_id": "UniRef90_UPI0001E32E7D", "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI0001E32E7D", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E7D" ], "product": "DUF4143 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_965", "locus": "GCA000148625_00938" }, { "type": "tRNA", "sequence": "contig_1", "start": 532615, "stop": 532699, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(aag)", "amino_acid": "Leu", "anti_codon": "aag", "score": 71.4, "nt": "GCCCGAGTGGTGAAATTGGCAAACACGCTAGACTAAGGATCTAGTGCTTAACGGCTTGCAGGTTCAAGTCCTGTCTCGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 532663, 532665 ], "id": "IGIGPGHMMC_32", "locus": "GCA000148625_00939" }, { "type": "cds", "sequence": "contig_1", "start": 532762, "stop": 533160, "strand": "-", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26", "UniRef:UniRef90_C4XF26" ], "nt": "TTGAAATATAGTGATTTAGGTCAAAAAACTAATGTAACTAAAATTTTAACTTTTGGTAAGGATGAAATAGCTACTAAAAATTTTAAGAACTTTTTAGCTAAAAAATATCCTTCACTTGCATTTCATATAGTTTCAAAAGGTTACTCAATTGAAATCACACATAAAAATGCAACCAAAGGTAAAGGTGCAGCATTTGTTTGTGACTTATTAAAAATAGATCCTAAAAACGCTGCTCACATTGGTGATAGCGGAAATGATTTAAATGCTTTACCTGAGATAGGAACATTCATTGCTATGGAAAATGCAATGGATGAGGTAAAAGAAGTAGCACATTATATAGGGCCTTCTTTTGAAAATAGTGGAATAGCCAAGCTATTTAAAGATTTAAAAGAAATATAA", "aa": "MKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI", "aa_hexdigest": "0c273b52d5694e192a0637ad9698282b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 532762, "stop": 533460, "observations": { "mutation": [ 533181 ], "stop": [ 533181 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF26", "query_cov": 1.0, "subject_cov": 0.43137254901960786, "identity": 0.992, "score": 265.0, "evalue": 3.24e-87, "gene_start": 1, "gene_end": 132, "reference_start": 175, "reference_end": 306, "reference_sequence": "MNIFYQYIYKTALLVNDWKLGNYRYIFFVSYIKSLYIIKIMKKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRPWTAKLEKLKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI" }, "paralog": false, "identity": 0.9956896551724138, "score": 458.0, "evalue": 6.52e-166, "description": "Internal stop codon at 533181. Nonsense mutation around 533181.", "psc": { "uniref90_id": "UniRef90_C4XF26", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF26", "UniRef:UniRef50_C4XF26" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26" ], "product": "COF family HAD hydrolase protein" } }, "id": "IGIGPGHMMC_966", "locus": "GCA000148625_00940" }, { "type": "cds", "sequence": "contig_1", "start": 533179, "stop": 533562, "strand": "-", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "nt": "ATGAGAAAAGAAATTCAAGCTTATTTTATAGATTTAGATGGAACATTATTAGACTTACCAGAAGGTGAAGAAACTCTTTCAAGTGCTAATGTTAAAGCTGTTGTTAAATTAAATAAAACTAAACCAGTTATTATTTCAACAGGTAGAAGTAATAGCGAATTTGTTTTATCCTTAGTTAAATTGTTAAAGTGCCCTTATGCCATTTGTCAAAATGGAGCTTTAATAGTTGATAAGAAAAATAAAGTTTTGGCTAGATATGAGATGGAACAAAATCAAGTTGATGAAATAGTTAAGCTTTTAAAAGAAGAAGAAATGTTTTTTATTTTTAATGGCGAAAATAAAATCTATTGTAACTATGAATCAAGTGCCTACATGCGCCCTTGA", "aa": "MRKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRP", "aa_hexdigest": "271171aad46acfe194f6ee29b8fd9cc1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KXX5", "query_cov": 0.8110236220472441, "subject_cov": 0.9716981132075472, "identity": 0.6890000000000001, "score": 152.0, "evalue": 7.95e-46, "valid": false, "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_A0A3P8KXX5" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "product": "COF family HAD hydrolase protein" }, "genes": [], "id": "IGIGPGHMMC_967", "locus": "GCA000148625_00941" }, { "type": "cds", "sequence": "contig_1", "start": 534776, "stop": 534958, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAAAAGAAAAATATAATGAATTTTTAGCTAAATTTCAAGATTTAAAAATTAATAAAATTTTTGTTAAAGATCTTCAACCTTTTTATTCAAGTAATGACAGTGAAACTATAAAGATTTTAGAATTCTATATTTATCTTTATAAAGCACTTTATTGTACTTTATCAAACTTTAATCATTAG", "aa": "MAKEKYNEFLAKFQDLKINKIFVKDLQPFYSSNDSETIKILEFYIYLYKALYCTLSNFNH", "aa_hexdigest": "6bfda7c657690474920cc67ba29a3c14", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7283.331599999998, "isoelectric_point": 7.810876655578613 }, "id": "IGIGPGHMMC_968", "locus": "GCA000148625_00942" }, { "type": "cds", "sequence": "contig_1", "start": 535331, "stop": 535606, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGATATAACAATTACACAAATGCAATTTAATGAATATTTATATGATTATAGTCAAAATCAAGAAAATAGAATTCATTTAGATGATTGGACTGCTTTTGATGAATATTATTATGAATTATTAAAAAAATATATCAAGGAAAAAGTCACTAATACTAATGAAAAAATAAGAATTTATTTCTTGCCTCATTATGATAATAAATATTATGCTTTTAGACTTAAATATGAAAGTGAAAAAGAAGCTGAAAGAGAATTAGGTAAAGAAGAATGA", "aa": "MKLDITITQMQFNEYLYDYSQNQENRIHLDDWTAFDEYYYELLKKYIKEKVTNTNEKIRIYFLPHYDNKYYAFRLKYESEKEAERELGKEE", "aa_hexdigest": "80511987ad1df00c8eb3f27370dfe5fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11490.685699999995, "isoelectric_point": 5.089613533020019 }, "id": "IGIGPGHMMC_969", "locus": "GCA000148625_00943" }, { "type": "cds", "sequence": "contig_1", "start": 535872, "stop": 535961, "strand": "+", "frame": 3, "gene": null, "product": "DUF262 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F0287F1", "UniRef:UniRef90_UPI000F0287F1" ], "nt": "ATGAAAGCAAACGATGAAAATATGTTAGATTATATGTTTATGAATCGTAGACAATATCACATACCTGTTTATCAAAGAAATTATGACTGA", "aa": "MKANDENMLDYMFMNRRQYHIPVYQRNYD", "aa_hexdigest": "584b38ca5252a9dff6e8c816f1fe23a4", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 535872, "stop": 536043, "observations": { "insertion": [ 536019 ], "mutation": [ 535959 ], "stop": [ 535959 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F0287F1", "query_cov": 1.0, "subject_cov": 0.5178571428571429, "identity": 1.0, "score": 65.9, "evalue": 9.89e-14, "gene_start": 1, "gene_end": 29, "reference_start": 1, "reference_end": 29, "reference_sequence": "MKANDENMLDYMFMNRRQYHIPVYQRNYDWKKENCILLYNDIIQAAKNDKNISLVL" }, "paralog": false, "identity": 0.9821428571428571, "score": 109.0, "evalue": 1.22e-33, "description": "Internal stop codon at 535959. Frameshift due to insertion around 536019. Nonsense mutation around 535959.", "psc": { "uniref90_id": "UniRef90_UPI000F0287F1", "product": "DUF262 domain-containing protein", "uniref50_id": "UniRef50_UPI000F0287F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F0287F1", "UniRef:UniRef50_UPI000F0287F1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F0287F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F0287F1" ], "product": "DUF262 domain-containing protein" } }, "id": "IGIGPGHMMC_970", "locus": "GCA000148625_00944" }, { "type": "cds", "sequence": "contig_1", "start": 536055, "stop": 536594, "strand": "+", "frame": 3, "gene": null, "product": "putative conserved protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXE5", "UniRef:UniRef90_A0A448ZXE5" ], "nt": "TTGAAAGAAGAAAATGGCGTAAACAATTATTTAATAATTGATGGGCAACAAAGAATGACATCAGTTTATCTTTTATTAAAAGCGTTATACGATTTAGAAACTCATCCATTTAATAAAGAGGATTTAGAAAGATTACTATTCAATTTTAGAGATTCAAAGAAATATTCAAATGATTTAATAGGAAAATTAAAATTAAAACCAATAAAAACAGATAACAAACAATTTGAATATTTAATGTCTGGTAATGTTGAAAAAATGGATGATACTTCAAACATTAAAATTAATTATGAATTATTTAAATTGCTAATAAAAGATTCAATTAAATGCAAAATAGAAACTTCTAAAATTTTAAGGGGAATAAAAAATTTAGAAGTTGTTATGATTACTTTGCAAGAAGAAAAAAATGGATTAGGTGACAATCCACAAGAAGTATTTGAAAGAATTAATTCAACTGGAGAAGATTTGACTTTATCTGATTTAATAAGAAACTTTGCATTAATGACTGATCTAGATAGAGAACAACTATTCGAAAATTATTGA", "aa": "MKEENGVNNYLIIDGQQRMTSVYLLLKALYDLETHPFNKEDLERLLFNFRDSKKYSNDLIGKLKLKPIKTDNKQFEYLMSGNVEKMDDTSNIKINYELFKLLIKDSIKCKIETSKILRGIKNLEVVMITLQEEKNGLGDNPQEVFERINSTGEDLTLSDLIRNFALMTDLDREQLFENY", "aa_hexdigest": "c4163af4e37d1cf79b28b326dc21e4b6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 535872, "stop": 536560, "observations": { "deletion": [ 536547 ], "mutation": [ 535959 ], "stop": [ 535959 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A448ZXE5", "query_cov": 0.9273743016759777, "subject_cov": 0.7155172413793104, "identity": 0.946, "score": 305.0, "evalue": 2.87e-103, "gene_start": 1, "gene_end": 166, "reference_start": 62, "reference_end": 227, "reference_sequence": "MKANDENMLDYMFMNRRQYHIPVYQRNYDWKKENCILLYNDIIQAAKNDKKHFFGSIVQVNLKEENGVNNYLIIDGQQRMTSVYLLLKALYDLETHPYNKEDLERLLFNFRDSKKYSNNLIGKLKLKPIKTDNKQFEYLMSDNVEKMDDTSNIKINYELFKSLIKDSIKSKIETSQILRGIKNLEVVMITLQEEKNGLGDNPQEVFERINSTGEDLTLSDLIRNFCINDWFR" }, "paralog": false, "identity": 0.9694323144104804, "score": 439.0, "evalue": 1.55e-158, "description": "Internal stop codon at 535959. Frameshift due to deletion around 536547. Nonsense mutation around 535959.", "psc": { "uniref90_id": "UniRef90_A0A448ZXE5", "product": "Uncharacterized conserved protein", "uniref50_id": "UniRef50_A0A448ZXE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A448ZXE5", "UniRef:UniRef50_A0A448ZXE5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXE5" ], "product": "Uncharacterized conserved protein" } }, "id": "IGIGPGHMMC_971", "locus": "GCA000148625_00945" }, { "type": "cds", "sequence": "contig_1", "start": 536595, "stop": 537302, "strand": "+", "frame": 3, "gene": null, "product": "DUF262 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F038BF0", "UniRef:UniRef90_UPI000F038BF0" ], "nt": "TTGCCTGTAGAAAATACTATTAAAAAAGATAAACTTAATGATTTTGTTTTAGCATATTTACTTTTCAAAATGCCAGACTCAACAACTGAAAAAAATGCATATCAAACTTTTAAAAAATATGTCGAAAAAAATCAAATTAGCAACAAAGAAATATTGGAAGATTTGAAAAAATATTCTAAATATTACAATGTTTTTATTAATGATGACTATAAAAATTATTCTAAAAAAACAAATAATTTATTAAGTGTTTTCAGAGTATTAAAACAAACAACAATTTATCCATTTTTATTTAGTGTTTTTGAAGATTATGAAAATTCAATAATAGATGAAAATGTTTTAAACAGTGTTCTACAATTTTTTGCCACATACATTATTAGAAGATCAATATGTTCTATTCCAACTAATTCCTTAAGAGGGTTGTTCAAAACACTTTACAAAAGAATTTTTCAAAATGAAAAAAGCAAAGAAGAATATTTAAAAAATTTATATGGCTTTATGGCTTCATTAACAAATACTAAAGATAGTGTTCCATCTAATGAAAATTTTGAAGATTCATTGAAAAAATTTGAATTGTACAAAAATTCAAAATTATGTAAATACTTACTTTCAACAATAGAAAATGGAAATTTGAATTTCAAAGAAAGAATTGAAATCAATGAAAACATTACTATTGAACACATTATGCCTCGTAACAAAACCTCTTATTGA", "aa": "MPVENTIKKDKLNDFVLAYLLFKMPDSTTEKNAYQTFKKYVEKNQISNKEILEDLKKYSKYYNVFINDDYKNYSKKTNNLLSVFRVLKQTTIYPFLFSVFEDYENSIIDENVLNSVLQFFATYIIRRSICSIPTNSLRGLFKTLYKRIFQNEKSKEEYLKNLYGFMASLTNTKDSVPSNENFEDSLKKFELYKNSKLCKYLLSTIENGNLNFKERIEINENITIEHIMPRNKTSY", "aa_hexdigest": "53683eb2964de45138d3b7bb43951d6e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 536295, "stop": 537605, "observations": { "mutation": [ 536592, 537300, 537486 ], "stop": [ 536592, 537300, 537486 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F038BF0", "query_cov": 1.0, "subject_cov": 0.44761904761904764, "identity": 0.983, "score": 450.0, "evalue": 2.67e-155, "gene_start": 1, "gene_end": 235, "reference_start": 181, "reference_end": 415, "reference_sequence": "MKEENGVNNYLIIDGQQRMTSVYLLLKALYDLETHPFNKEDLERLLFNFRDSKKYSNDLIGKLKLKPIKTDNKQFEYLMSGNVEKMDDTSNIKINYELFKLLIKDSIKCKIETSKILRGIKNLEVVMITLQEEKNGLGDNPQEVFERINSTGEDLTLSDLIRNFALMTDLDREQLFENYWLPVENTIKKDKLNDFVLAYLLFKMPDSTTEKNAYQTFKKYVEKNQISNKEILEDLKKYSKYYNVFINDDYKSYSKKTNNLLSVFRVLKQTTIYPFLFSVFEDYENSIIDENVLNSVLQFFVTYIIRRSICSIPTNSLRGLFKTLYKRIFQNEKSKEEYLKNLYDFMASLTNTKDSVPSNENFEDSLKKFELYKNSKLCKYLLSTIENGNLNFKERIEINENITIEHIMPRNKTSYWRNEIGANYDAVYDKYLNTIGNLTLTGYNSELSDKPFKSKKISIEEFGKFNTLNKDIKNCTIWNEDSIVKRANRLSNIVCEIFSLPQEVFDYKIVEDEIKQINIDDKKLI" }, "paralog": false, "identity": 0.9862385321100917, "score": 848.0, "evalue": 6.97e-315, "description": "Internal stop codon at 536592, 537300, 537486. Nonsense mutation around 536592, 537300, 537486.", "psc": { "uniref90_id": "UniRef90_UPI000F038BF0", "product": "DUF262 domain-containing protein", "uniref50_id": "UniRef50_UPI000F038BF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F038BF0", "UniRef:UniRef50_UPI000F038BF0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F038BF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F038BF0" ], "product": "DUF262 domain-containing protein" } }, "id": "IGIGPGHMMC_972", "locus": "GCA000148625_00946" }, { "type": "cds", "sequence": "contig_1", "start": 537522, "stop": 537977, "strand": "+", "frame": 3, "gene": null, "product": "DUF1524 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC2", "UniRef:UniRef90_A0A448ZXC2" ], "nt": "TTGTCTAATATTGTTTGTGAAATATTTTCTTTACCTCAAGAAGTATTTGACTATAAAATAGTAGAAGATGAAATAAAACAAATTAATATTGATGACAAAATTGATTTAACTTTCACAAAACCTGTTTATTTCAACTTATTAGGTGAAATGGTTGATTCAACTTCTTATGGTGAGCTAATTAAAAAAGTATCAAATATTTTATATTATCAAGATCCAATGACAATGAAAAAATTGGCTCAAGATAACTACAAACTTCCTAATGGAACAAGTGTACAAATAACAAATGATCCTAAAAAATTAAACAAAGCTATAGAAATCGATGAAAATAGTGGAATTTACATTGAAGGCACAAAAAATGCAAATGCTGTTATAACATATATTAAAGCAATTTTATCAGAATTTGATATTTCACATGATGACTTCATTGTATTTACAAAAAAAGTTAAAAATAAATAA", "aa": "MSNIVCEIFSLPQEVFDYKIVEDEIKQINIDDKIDLTFTKPVYFNLLGEMVDSTSYGELIKKVSNILYYQDPMTMKKLAQDNYKLPNGTSVQITNDPKKLNKAIEIDENSGIYIEGTKNANAVITYIKAILSEFDISHDDFIVFTKKVKNK", "aa_hexdigest": "09eadd492f354fdce5805cc95755917b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 537222, "stop": 537977, "observations": { "mutation": [ 537300, 537486 ], "stop": [ 537300, 537486 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A448ZXC2", "query_cov": 1.0, "subject_cov": 0.511864406779661, "identity": 0.927, "score": 280.0, "evalue": 4.16e-93, "gene_start": 1, "gene_end": 151, "reference_start": 145, "reference_end": 295, "reference_sequence": "MASLANTKDGVPSNKDFEDSLKKFELYKNSKLCKYLLSTIENGNLNFKERIEIDENITIEHIMPRNKTSYWRNEVGTNYDEVYDKYLNTLGNLTLTGYNSELSDKPFKNKKISIEEFGKFNTLNKDIKNCAIWNEDSIVKRADRLANIVCEIFSLPQEVFDYKIVEDEIKQINIDDKIDLTFTKPVYFNLLGEMVDLTSYGDLIKKVSNILYYQDSTTMKKLAQNNWKLPNGTSVQITNDPKKLNKAIEIDENSGIYIEGTKNANAVITFIKAILSEFDIAHDDFTVFTKKVKNK" }, "paralog": false, "identity": 0.9203187250996016, "score": 464.0, "evalue": 4.76e-168, "description": "Internal stop codon at 537300, 537486. Nonsense mutation around 537300, 537486.", "psc": { "uniref90_id": "UniRef90_A0A448ZXC2", "product": "Protein of uncharacterized function (DUF1524)", "uniref50_id": "UniRef50_A0A448ZXC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A448ZXC2", "UniRef:UniRef50_A0A448ZXC2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC2" ], "product": "Protein of uncharacterized function (DUF1524)" } }, "id": "IGIGPGHMMC_973", "locus": "GCA000148625_00947" }, { "type": "cds", "sequence": "contig_1", "start": 538191, "stop": 538550, "strand": "-", "frame": 3, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "RefSeq:WP_013354694.1", "SO:0001217", "UniParc:UPI0001E32E82", "UniRef:UniRef100_UPI0001E32E82", "UniRef:UniRef50_UPI0001E32E82", "UniRef:UniRef90_UPI0001E32E82" ], "nt": "ATGCATAAAATTTTAACTGGAAAATTATATTTAGACAAATTGATTAATAATCTAAATAACACCAAAATTAAACTTGTAACTGGTTACAAATCAGTTGGAAAAAGTACAATACTTAAACAATTAGCCGACTATATTGAAAAAAATATAGATAACTCAAATCTAATTTATATTGATGATTTTTACATCAAAAAATTTAAAACTTATCAAGAAGCAAAAGATTACATTTTTGCAAAACATAAAGTTGATAAAGTCAACTATTTATTAATAGATGACATACAAGTGTGTCCAGATTTTAAACAATTGGTAAATGATATGAGACTGTTAGAATTTTGTGTAAATTCTATCCTTTTTTCAATATAG", "aa": "MHKILTGKLYLDKLINNLNNTKIKLVTGYKSVGKSTILKQLADYIEKNIDNSNLIYIDDFYIKKFKTYQEAKDYIFAKHKVDKVNYLLIDDIQVCPDFKQLVNDMRLLEFCVNSILFSI", "aa_hexdigest": "66fff2cf8c6e6dcfa9d9f3f731f8954c", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001E32E82", "ncbi_nrp_id": "WP_013354694.1", "uniref100_id": "UniRef100_UPI0001E32E82", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E82", "RefSeq:WP_013354694.1", "UniRef:UniRef100_UPI0001E32E82" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32E82", "uniref90_id": "UniRef90_UPI0001E32E82" }, "psc": { "uniref90_id": "UniRef90_UPI0001E32E82", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI0001E32E82" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E82" ], "product": "AAA family ATPase" }, "genes": [], "id": "IGIGPGHMMC_974", "locus": "GCA000148625_00948" }, { "type": "cds", "sequence": "contig_1", "start": 538675, "stop": 539250, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12008.13" ], "nt": "ATGCAAGACTATCAAAGTCTTTATTTAGAAATAAAAGATGAATTTGAAACTCGTCAAAAAAGTGAAGCAGTTTCAATTTTGGATGATGTTGTCTTTCAAATTGAACTTGTTAAAGCTAATGATGTTAATGTAGATTACATCTTAGGCCAAGTTAAGGATGCACATGAAAAAAATATTACTGAAAAAGAATTGATAGTTCAAATTGAGAGAATTGTTAAATCAAGTTTAAACTTGAAAAACAAAGAAGAATTAATTAAAAGATTTATTCGTACAGTCAACTTCGATAATGATTTTGACAAATTAAGTATTCTCGACTTAAAAGAAGCCTTTGACAAATTTGTTGCTTTAGATAAAGAAGTGGCAGTTAATGAATTAATAGCTAAATTTAATCTTAAAGTTAAAGAAGCTAAAAAGTTTATTCTTAACGCTATTGAAAACAATGAACTTAATAACTATGGAACAAAATTCACAAATATTTTACCTGCTCTTGGACTTTTTGATAAAAGAAGAGAAACCATCAAAGTTCAAGTTAGTGAAGCGATTCAAAACCTTATAGATTTATATAATGAACAATAA", "aa": "MQDYQSLYLEIKDEFETRQKSEAVSILDDVVFQIELVKANDVNVDYILGQVKDAHEKNITEKELIVQIERIVKSSLNLKNKEELIKRFIRTVNFDNDFDKLSILDLKEAFDKFVALDKEVAVNELIAKFNLKVKEAKKFILNAIENNELNNYGTKFTNILPALGLFDKRRETIKVQVSEAIQNLIDLYNEQ", "aa_hexdigest": "7252b6ac5e952ab3cfde7f6642130a5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12008.13", "name": "EcoR124_C", "length": 191, "aa_cov": 0.9738219895287958, "hmm_cov": 0.9738219895287958, "evalue": 6.451538683302888e-33, "score": 110.42512512207031, "start": 1, "stop": 186 } ], "seq_stats": { "molecular_weight": 22258.22170000002, "isoelectric_point": 4.985996055603027 }, "id": "IGIGPGHMMC_975", "locus": "GCA000148625_00949" }, { "type": "cds", "sequence": "contig_1", "start": 539284, "stop": 539436, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12008.13" ], "nt": "ATGCCTTCATATTTAAGTTTAGTCAATAGACTTCAACATGAATTTCCTCTTGATCAATATATCTATGAAGATCCAGCTAAAAAATTATTTGTTAAAACCTTTGGTCAAATACTTTATCTTTTGAACATTCTTAAAGTCTTTACTGAAGAGTGA", "aa": "MPSYLSLVNRLQHEFPLDQYIYEDPAKKLFVKTFGQILYLLNILKVFTEE", "aa_hexdigest": "60c4fc6f487503d250d79ae5e97e641b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12008.13", "name": "EcoR124_C", "length": 50, "aa_cov": 0.94, "hmm_cov": 1.0, "evalue": 1.432362586937319e-07, "score": 27.351961135864258, "start": 3, "stop": 49 } ], "seq_stats": { "molecular_weight": 6006.958499999999, "isoelectric_point": 5.675396156311035 }, "id": "IGIGPGHMMC_976", "locus": "GCA000148625_00950" }, { "type": "cds", "sequence": "contig_1", "start": 539653, "stop": 539898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22679.2" ], "nt": "TTGAGTGAAACTGATGAAAACAATGAATCAACTAAAAATTTAGATCCTAGCTCTAAAGAATTTTTATCTAATGCTATTAAAGATTACAACAAAATGTTTGGCACTAATTATGATATTAGTGATGACAAATTTAATAACTACTATAAAGATGTAAGTTTAAGAATGAAAAACAAAGAAATTGATCTTTTAATAGTTGTCAATATGTTTTTAACAGGTTTTGATGCCACAACATTAAATACTTTATGA", "aa": "MSETDENNESTKNLDPSSKEFLSNAIKDYNKMFGTNYDISDDKFNNYYKDVSLRMKNKEIDLLIVVNMFLTGFDATTLNTL", "aa_hexdigest": "4ebe46399255b814e7d25db572076004", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_T1D0F5", "query_cov": 0.8395061728395061, "subject_cov": 0.8095238095238095, "identity": 0.691, "score": 92.4, "evalue": 5.55e-23, "valid": false, "product": "Type I restriction-modification system, restriction subunit R", "uniref50_id": "UniRef50_T1D0F5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22679.2", "name": "T1R_D3-like", "length": 81, "aa_cov": 0.9506172839506173, "hmm_cov": 0.9629629629629629, "evalue": 3.772306684576155e-26, "score": 88.41900634765625, "start": 5, "stop": 81 } ], "seq_stats": { "molecular_weight": 9375.336599999995, "isoelectric_point": 4.4735925674438475 }, "id": "IGIGPGHMMC_977", "locus": "GCA000148625_00951" }, { "type": "cds", "sequence": "contig_1", "start": 539941, "stop": 540783, "strand": "-", "frame": 2, "gene": null, "product": "Helicase ATP-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0V8QIP8" ], "nt": "ATGAACTTTATTGATAAAGTACTATTTGTTGTCGATAGAAAAGATTTAGACTATCAAACAATGGTTGAATATGAAAAATTTGAAAAAGGTGCAGCTAATGCAACTACTAATACTTCAATATTGAATGACAAGTTAACTTCAACTGGTTTAAATTCAAAAGTTATTATTACAACAATTAATAAACTTTCAAATCTAGTTAAAAAATATGCAACTCATCCTATTTACAATAAAAATGTAGTCATTATTTTTGATGAATGTCACCGTTCACAATTTGGCGATATGCACAAAAATATTATTAAAAAATTTAAAAATTATTATATCTTTGGCTTTACTGGCACTCCTATTCTTTCAAGATTTGCCAAAATGCCTAGAGGTGGTGGAATTCCTTTAACAACCGATGAAATCTTTGGCACAAGACTTCATGCTTATACTATTGTCGACGCCATCAAAGATAATAATGTTTTATCTTTTAAAATTGATTACATTAATACTATTAAGGCAAAAGAACAAATTGAATCTCAACTTGTTCCAGATATTAAAGCTGAAGAAGCTCTTAGAGCTCCAGCAAGAATTGAAGCTATAGTCCAAGATATTCTTAAAAACTATAAAGCTAAAACTAGAAGAAATGATAATAAATATTCTTTTTATGCTATTTCCAATATTGAAGAAACAATCAATAGAGGAGAAAAAGAAGTTAAATTATTGAAAGAATTTGAAGGTTTCAATGGAATGCTTGCAACTTCTTCAATTGATTCAGCTAAAGCTTACTATAAAGAATTTAAAGTACAACAATCTAAACTTCCAGAAAGTGAACGTCTTAGAATTGCTTTAATTTATAGTTGA", "aa": "MNFIDKVLFVVDRKDLDYQTMVEYEKFEKGAANATTNTSILNDKLTSTGLNSKVIITTINKLSNLVKKYATHPIYNKNVVIIFDECHRSQFGDMHKNIIKKFKNYYIFGFTGTPILSRFAKMPRGGGIPLTTDEIFGTRLHAYTIVDAIKDNNVLSFKIDYINTIKAKEQIESQLVPDIKAEEALRAPARIEAIVQDILKNYKAKTRRNDNKYSFYAISNIEETINRGEKEVKLLKEFEGFNGMLATSSIDSAKAYYKEFKVQQSKLPESERLRIALIYS", "aa_hexdigest": "6c83a64fa3b24dc9b9caf173658b194f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0V8QIP8", "query_cov": 1.0142857142857142, "subject_cov": 0.8452380952380952, "identity": 0.504, "score": 256.0, "evalue": 7.54e-81, "valid": false, "product": "Helicase ATP-binding domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A0V8QIP8", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A0V8QIP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0V8QIP8" ], "product": "Helicase ATP-binding domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_978", "locus": "GCA000148625_00952" }, { "type": "cds", "sequence": "contig_1", "start": 540844, "stop": 540954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGAGACCATATCAAATTGTTGCTACTGAACGAATTATTGAAAAAATTAGAGCTGCTTACAATATGAAACAACAAGGTACTAGAGACGCAGGCGGTTACATCTGA", "aa": "MVMRPYQIVATERIIEKIRAAYNMKQQGTRDAGGYI", "aa_hexdigest": "e677483d141344be3fcc4d39bbc7491f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4172.854400000001, "isoelectric_point": 9.815069770812986 }, "id": "IGIGPGHMMC_979", "locus": "GCA000148625_00953" }, { "type": "cds", "sequence": "contig_1", "start": 541582, "stop": 541836, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACTAATCAAATTAAAGAAAAAGAGCTTATAATGCAAAACTCAGATAGTACAGTTCTAGCTGAGTATATTTCAACTAAAGAAAAATTTTCTGAATATGAAAGCGAAGCTCAATTGGAAGCAAGTTTTGTTAAGCAACTACTTCAACAAGGATATGAAAAAATAAACATTAGAGATGAAAATGATTTAATCTTAAATTTAAGAAAACAAATTGAAAAATTAAATGATGTTAAATTTAATGATCGTGAATGA", "aa": "MKTNQIKEKELIMQNSDSTVLAEYISTKEKFSEYESEAQLEASFVKQLLQQGYEKINIRDENDLILNLRKQIEKLNDVKFNDRE", "aa_hexdigest": "34f816914236e89221f245ae947187fa", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9986.13109999999, "isoelectric_point": 4.923757362365721 }, "id": "IGIGPGHMMC_980", "locus": "GCA000148625_00954" }, { "type": "cds", "sequence": "contig_1", "start": 541874, "stop": 542515, "strand": "-", "frame": 1, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "nt": "TTGTTAAATTTTAGCAACTTTAATAAAAATAATTCAAAAGAATTACAATCTATAGGCTGTTTTATTAGTGGTTTAAGATCAAAAAATAAAGATAGTTTTGTCGATGGAAACCAAAGATATATTTCTTATTTAGATGTTTTCAATAACAAAGAAATTAATCATTTACCTAACAATTTTGTAAAAATATTTGATGATGAAAATCAAAACAATCTTAATTATGGCGATGTTATTTTTTGTGGTTCATCAGAAAATTTTGATGAAACAGGTTATGCATCTGTTTACACTATAAAAAGTGATGAAAAAGTTTATTTAAATAGTTTTTCGTTTATTTTTAGATTTAAAGATAATGAATTATTTTTGCCTAAATTTTCTAAATATTTTTTTAATTGTAAAGACTTTAGGGACTTACTTCTCAAATGTATAAATGGTGTAACTAGATTTAATTTATCAAAAGAAAAGATGTCAAAAATAAAAATACCTATTCCTCCTCTCAAAACCCAAAACAAGATAGTTTCAATTCTTGACAAACTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTATAGAGATCAATTATTAAACTTTAAAAATAATGAATAA", "aa": "MLNFSNFNKNNSKELQSIGCFISGLRSKNKDSFVDGNQRYISYLDVFNNKEINHLPNNFVKIFDDENQNNLNYGDVIFCGSSENFDETGYASVYTIKSDEKVYLNSFSFIFRFKDNELFLPKFSKYFFNCKDFRDLLLKCINGVTRFNLSKEKMSKIKIPIPPLKTQNKIVSILDKLSEYSQEINSGLPAEIELRSKQFKYYRDQLLNFKNNE", "aa_hexdigest": "be0df030026e370ed5cf4a8f92fc80bf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A5B8JXU3", "query_cov": 0.9295774647887324, "subject_cov": 0.8284518828451883, "identity": 0.525, "score": 203.0, "evalue": 1.2e-62, "valid": false, "product": "Restriction endonuclease subunit S", "uniref50_id": "UniRef50_A0A5B8JXU3" }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JXU3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "IGIGPGHMMC_981", "locus": "GCA000148625_00955" }, { "type": "cds", "sequence": "contig_1", "start": 542525, "stop": 542710, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGAAAAACTTGAATATAAGTCCTTCGTCAAAATCGGGTATTCCACAATTAACCAGAAAACATATAGAAAATATTAAAATTCCTTTAATTCCTTTAAAAATTCAAGAAAAAATAGTCGAAATCTTAGAGAGATTTCGTATACTAGAAGCGGAGTTGGAAGCGAGAGGGAAGCAATTTGATTTCTGA", "aa": "MKNLNISPSSKSGIPQLTRKHIENIKIPLIPLKIQEKIVEILERFRILEAELEARGKQFDF", "aa_hexdigest": "2ee4ae4efb8029019fa7055295f8c8a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 0.8688524590163934, "hmm_cov": 0.9811320754716981, "evalue": 1.887886827229158e-10, "score": 37.23214340209961, "start": 2, "stop": 54 } ], "seq_stats": { "molecular_weight": 7112.344199999998, "isoelectric_point": 9.59813365936279 }, "id": "IGIGPGHMMC_982", "locus": "GCA000148625_00956" }, { "type": "cds", "sequence": "contig_1", "start": 542738, "stop": 543052, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGACTTATTTAGAAAAATTGATAAATAAGTATTGTCCTGACGGATATGAGTGGAAGGATCTAAAAGATATTACTAAAATTTATGTAGGCGGAGATTTACCAAAAAAATCTTTTTCAGAAACAAAAAATGAAAATTTCAATGTAAAAATTTTAACTAATGGATCTTTTGGAAATAATATTAAAGGATATACCGATTCCTATGTAGTGCCTGGTAATTCAATAACAATTTCTGCAAGAGGAACAATAGGCTATTGTGAATACCAAAATGAACCTTTTTACCCAATTATTAGACTTCTGGCAATTCATCCAACTTGA", "aa": "MTYLEKLINKYCPDGYEWKDLKDITKIYVGGDLPKKSFSETKNENFNVKILTNGSFGNNIKGYTDSYVVPGNSITISARGTIGYCEYQNEPFYPIIRLLAIHPT", "aa_hexdigest": "1eab182c7835b5409577bd38df9a1d61", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 86, "aa_cov": 0.7884615384615384, "hmm_cov": 0.9534883720930233, "evalue": 7.679509963100332e-14, "score": 48.26430892944336, "start": 13, "stop": 94 } ], "seq_stats": { "molecular_weight": 11838.332799999987, "isoelectric_point": 7.699091529846191 }, "id": "IGIGPGHMMC_983", "locus": "GCA000148625_00957" }, { "type": "cds", "sequence": "contig_1", "start": 543054, "stop": 543581, "strand": "-", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "nt": "ATGCACATTCTACACTTATTGAGTGCTAAAGGAACAGCTGCTATAGTTGAGTTCCCTGGTGTTCTTTATCGTGGTGGTGCTGAACAAAAAATTAGACAATACTTAATTGATAATAATTATGTAGATGCTATTATTCAACTTCCTTCAAACTTATTCTTTGGTACTTCAATAGCAACCTGCATTATTGTTTTAAGAAAAAACAAAAAAGATAGCGACATCTTATTTATTAATGCTTCAAATGAATTTATTAAAGAAAAAAATAACAATAAACTAACTGATGAAAATATCAATAACATTCTTGATATTTATCGTTATACTAAAGAAAAAGAATATGTTTCAAGAAATGTACCTTATGAAGAAGTAAAAGAAAATGATTATAGCTTATCAGTTTCTACTTATGTTAAAGCAAAAGATACTTCAGAGAAAATTGATATTGTTCAAGTTAATGCACAATTAAAAGAATTAACTAAGAAAATAGATGTTTTAAGAAAAGAAATTGACAAAATAATTGAAGAAATTGAGGATTAA", "aa": "MHILHLLSAKGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAIIQLPSNLFFGTSIATCIIVLRKNKKDSDILFINASNEFIKEKNNNKLTDENINNILDIYRYTKEKEYVSRNVPYEEVKENDYSLSVSTYVKAKDTSEKIDIVQVNAQLKELTKKIDVLRKEIDKIIEEIED", "aa_hexdigest": "bf05cda98eb249a3c6bab72fe74c254e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025CE0BCD", "query_cov": 0.9942857142857143, "subject_cov": 0.8018433179723502, "identity": 0.632, "score": 205.0, "evalue": 2.53e-64, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A132HPU2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A132HPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "IGIGPGHMMC_984", "locus": "GCA000148625_00958" }, { "type": "cds", "sequence": "contig_1", "start": 543675, "stop": 544532, "strand": "-", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "TTGGGATTTTTATTCTATCGTTATATTTCTGAAAATTTAACCAATTTTGTTAACACAAAACAAGAAGAAGCTGGTGTTAAAGATTTTGATTATGCTGAAATTAGTGATAAACAAGCAAATGAAATAAGAAACACATTAATTCAAGAAAAAGGTTTCTTTATTTTACCAAGTAAACTATTTAAAAATGTTGCTAAGAATTGTCGCAATGATTTAAATTTAAATGAAACATTAGACAATATTTTTAAAGGTATTGAAGCTTCAGCTATTGGTACACCAAGTGAAAATGACATTAAAGGTTTATTTAATGATGTTAATGTTAACAATGATAAATTAGGGAATAATGTTATTGAAAGAAACAAAAAATTAAGTGGAATTCTTGAAGCAATCAATGAAATGCAACTAGGAGATTTCCATGGTCACAACATTGACGCTTTTGGCGATGCATATGAATATTTAATGACCATGTATGCAGCAAATGCCGGTAAAAGTGGTGGTGAATTCTTTACACCTCAAGAAGTTAGTGAGCTTTTAGCTTCTTTAACATTAGTTGATTTTAGTAGTGATAAAAGAGAAATGAAAAAAGAAGTTGATAGTGTCTACGACCCTGCTTGTGGTTCAGGTTCATTATTGCTTAAATTTGCTAAGATTTTAGGTAAAGATAAGGTTACAAAAGGTTTTTTTGGACAAGAAATTAATTTAACAACTTATAACTTAGCAAGAATTAATATGTTTTTACACGGTATTAACTTTGCTGATTTCTCAATTAAACATGGAGATACATTAAATCATCCTCAATATTTTGAAAATGTTAAGAATTTCGAAGCAATAGTATCTAATCCTCCTTATTCTATTAAATGA", "aa": "MGFLFYRYISENLTNFVNTKQEEAGVKDFDYAEISDKQANEIRNTLIQEKGFFILPSKLFKNVAKNCRNDLNLNETLDNIFKGIEASAIGTPSENDIKGLFNDVNVNNDKLGNNVIERNKKLSGILEAINEMQLGDFHGHNIDAFGDAYEYLMTMYAANAGKSGGEFFTPQEVSELLASLTLVDFSSDKREMKKEVDSVYDPACGSGSLLLKFAKILGKDKVTKGFFGQEINLTTYNLARINMFLHGINFADFSIKHGDTLNHPQYFENVKNFEAIVSNPPYSIK", "aa_hexdigest": "a8bd9c98663bf0a8b2aae00c15ce10a7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8431952662721893, "identity": 0.607, "score": 338.0, "evalue": 2.75e-113, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "IGIGPGHMMC_985", "locus": "GCA000148625_00959" }, { "type": "cds", "sequence": "contig_1", "start": 544633, "stop": 545145, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI0001E32E86", "UniRef:UniRef90_UPI0001E32E86" ], "nt": "TTGACAATCAAATCAATTGATGAAAATCTAAATGTGATCAATAGCCAATTAGATAATTTTTATTCTAATGATCATATTGATAGTCAATTTTTTACTAAAGAAGGCGACATCATTGTTCGAAACATGTATCCTTATGAAGTTGCCTTAATAAAAAAAGAAGATCAAGGTATTTTAATTAGTACTAATTTTATAGTGATTAGAAACCTAGAAGGATTATTACCTAAATACTTAGCTTATTTATTAAGCATTGATGTAATTAAAGATTTATTGGTCTTTAAATCAGCTGGATCAGTATCAAAGCATATAAATAATAAGATACTAGGCTCACTTAATGTCAAAGTAATTTCATTAAATGAACAACAAAGAATAATTGATTATGTTGATAACTCCTATAAAGTTAATAACTTGTATCAAGAAGCTATTGACTTAGAGAAAAAAAGAATTGAATACTACTTGACTAAAAGTCAAGATGAAAAAAAGGAAGGAATTAAATATGGCAAAAATTATAAATAA", "aa": "MTIKSIDENLNVINSQLDNFYSNDHIDSQFFTKEGDIIVRNMYPYEVALIKKEDQGILISTNFIVIRNLEGLLPKYLAYLLSIDVIKDLLVFKSAGSVSKHINNKILGSLNVKVISLNEQQRIIDYVDNSYKVNNLYQEAIDLEKKRIEYYLTKSQDEKKEGIKYGKNYK", "aa_hexdigest": "0a579fa3e73702b80c999a350efee9ae", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E86", "query_cov": 1.0, "subject_cov": 0.845771144278607, "identity": 0.9940000000000001, "score": 321.0, "evalue": 2.73e-110, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI0001E32E86", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E86" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "IGIGPGHMMC_986", "locus": "GCA000148625_00960" }, { "type": "cds", "sequence": "contig_1", "start": 545770, "stop": 546183, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E87", "UniRef:UniRef90_UPI0001E32E87" ], "nt": "ATGTTAGAAATTTTAAAAATATTAAATAATTATGACTATATTGCACAAATTTGTGATTTTAAATTATATGAATATAGAAATAAAAATAATAACCCAATAAAACAATTCATAATAGTTTTTTGTCCAACTTTTTATACAGAATTATTCCAAAAAAATCAAAATTTTAAATCTCGTATAGAAAACAAATTTGATTTAATAGATAAATATAATGAATCAAAAAAATTTAGAAAAAAATTTTATAAGGAATTAAAGTCCGATTTGTTTTCTATTTGCATTGAATTTTTTTTAGAAAAAGAAGTTCAAAATCTTCATAAATATGTAATTAATTTTAAAGGTAATAAAATTTCAGATGTTTATTGTTTTTTTGAGAAAAATGATTCTGAAAATGATTTTCTATATAAAAAAGAACTATGA", "aa": "MLEILKILNNYDYIAQICDFKLYEYRNKNNNPIKQFIIVFCPTFYTELFQKNQNFKSRIENKFDLIDKYNESKKFRKKFYKELKSDLFSICIEFFLEKEVQNLHKYVINFKGNKISDVYCFFEKNDSENDFLYKKEL", "aa_hexdigest": "0db7485feff460367f2d9569206f224f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 545590, "stop": 546483, "observations": { "mutation": [ 545619, 545736, 545772, 546204, 546237, 546249 ], "stop": [ 545619, 545736, 545772, 546204, 546237, 546249 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32E87", "query_cov": 1.0, "subject_cov": 0.43769968051118213, "identity": 1.0, "score": 280.0, "evalue": 5.96e-93, "gene_start": 1, "gene_end": 137, "reference_start": 117, "reference_end": 253, "reference_sequence": "MIWNILTFVLAIITFVFYSLYKTIKKFLIKKSFDAVSEVYRMYSSATSTDNSFLYISATFIDQKLFFCSFSDTILDLTKKNDELDPFSFYDYSLWEKQWFFNIIYFYKFWKVVKNKMLEILKILNNYDYIAQICDFKLYEYRNKNNNPIKQFIIVFCPTFYTELFQKNQNFKSRIENKFDLIDKYNESKKFRKKFYKELKSDLFSICIEFFLEKEVQNLHKYVINFKGNKISDVYCFFEKNDSENDFLYKKELWENEVKIESEIKWIEKSFNLDLKNKCEIVSKMIDEKREFFEREKENKKTMEWYRQMGVLD" }, "paralog": false, "identity": 0.9797979797979798, "score": 581.0, "evalue": 3.86e-214, "description": "Internal stop codon at 545619, 545736, 545772, 546204, 546237, 546249. Nonsense mutation around 545619, 545736, 545772, 546204, 546237, 546249.", "psc": { "uniref90_id": "UniRef90_UPI0001E32E87", "uniref50_id": "UniRef50_UPI0001E32E87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32E87", "UniRef:UniRef50_UPI0001E32E87" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E87" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16940.35779999999, "isoelectric_point": 8.73741626739502 }, "id": "IGIGPGHMMC_987", "locus": "GCA000148625_00961" }, { "type": "cds", "sequence": "contig_1", "start": 546247, "stop": 546474, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAACAATCAAAAAATTTTTAATAAAAAAGTCTTTTGATGCTGTAAGTGAAGTTTATAGAATGTATTCATCTGCGACTTCAACAGATAATAGTTTTTTATATATTTCAGCAACTTTTATAGATCAAAAATTATTTTTTTGCTCCTTTTCAGATACCATATTAGATTTAACCAAAAAAAATGATGAATTAGATCCATTTAGTTTTTATGATTATTCTTTATGA", "aa": "MYKTIKKFLIKKSFDAVSEVYRMYSSATSTDNSFLYISATFIDQKLFFCSFSDTILDLTKKNDELDPFSFYDYSL", "aa_hexdigest": "50ce129fdc58d846ca5f0fca6eb13d61", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8834.955099999994, "isoelectric_point": 5.116498374938964 }, "id": "IGIGPGHMMC_988", "locus": "GCA000148625_00962" }, { "type": "cds", "sequence": "contig_1", "start": 546706, "stop": 547038, "strand": "+", "frame": 1, "gene": null, "product": "protein adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "nt": "ATGGAAAATTCAATAGATCAAAAAAGTAAAATAAAAGCTTATAACTTATTTAACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCTGAAAGTAATTTGGAGCAAATAATAGATAAGTATATTGAAATGAATATAGCACATCCATTTCTAGAAGGCAATGGCCGAAGTACTAGAATCTGA", "aa": "MENSIDQKSKIKAYNLFNLIIEDKLEIGTTKSLMQIHGYLFGGLYSFAGQIRKKDISKGGFRFANSTYLNETLKNIENMPESNLEQIIDKYIEMNIAHPFLEGNGRSTRI", "aa_hexdigest": "8a99fb565ab28a5751d00e36e7c9abdb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6G5QHC3", "query_cov": 0.990909090909091, "subject_cov": 0.8320610687022901, "identity": 0.596, "score": 132.0, "evalue": 7.2e-38, "valid": false, "product": "protein adenylyltransferase", "uniref50_id": "UniRef50_A0A6G5QHC3" }, "pscc": { "uniref50_id": "UniRef50_A0A6G5QHC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "product": "protein adenylyltransferase" }, "genes": [], "id": "IGIGPGHMMC_989", "locus": "GCA000148625_00963" }, { "type": "cds", "sequence": "contig_1", "start": 547117, "stop": 547242, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ], "nt": "ATGCAAGAAAGTCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGAATTGATTATTCTTACTACTATGAAGAGTAA", "aa": "MQESPYDDTKIKNLIKKSLTDKINDRELFMKGIDYSYYYEE", "aa_hexdigest": "c7979606339603708649a7e1e6577ae1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A134CFU1", "query_cov": 1.0, "subject_cov": 0.803921568627451, "identity": 0.585, "score": 48.5, "evalue": 1.11e-06, "valid": false, "uniref50_id": "UniRef50_A0A134CFU1" }, "pscc": { "uniref50_id": "UniRef50_A0A134CFU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.603400000001, "isoelectric_point": 4.920460700988768 }, "id": "IGIGPGHMMC_990", "locus": "GCA000148625_00964" }, { "type": "cds", "sequence": "contig_1", "start": 547615, "stop": 547848, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATTAAACCTAAGGAATTATTTGACAAAGTTAAAGAAGCAATTAAAAATAAAGAAGTAGGCGACAAAAGTGACTATAGATATAAACTAAAAGGTAATTTAGCAAATATTAAATATGCCAGATTATATAAATATTTAATGGATATAAATAATATGATTCTTGCTAATTTATGTGATTTAGTTAGAAATGAAGATATATCTAAAAGTTCTTTAGGTGGTAAGCCTTGA", "aa": "MKEIKPKELFDKVKEAIKNKEVGDKSDYRYKLKGNLANIKYARLYKYLMDINNMILANLCDLVRNEDISKSSLGGKP", "aa_hexdigest": "04c3523b2ee5cb5a275f57829b2fcb6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8925.381299999995, "isoelectric_point": 9.459913444519042 }, "id": "IGIGPGHMMC_991", "locus": "GCA000148625_00965" }, { "type": "cds", "sequence": "contig_1", "start": 548329, "stop": 548517, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTATTTAATATTATAACTTCAGATGACATTAATGATGAAGTTGGTTGCGACATCATGGAAGAATATGAATTAGAAATGTATGAAGAAGTTAGAAATTATATAAAACGCGAAATAGGCAACAAAAAAACACCTAAAGTAGTGGCTTATCAACTTTGTGATTCAAAAGGTAAAAGTATTTGA", "aa": "MKKLFNIITSDDINDEVGCDIMEEYELEMYEEVRNYIKREIGNKKTPKVVAYQLCDSKGKSI", "aa_hexdigest": "c0bda3c361c98ff7eca50071293b66ef", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7279.280800000001, "isoelectric_point": 4.850491905212402 }, "id": "IGIGPGHMMC_992", "locus": "GCA000148625_00966" }, { "type": "cds", "sequence": "contig_1", "start": 548845, "stop": 549447, "strand": "+", "frame": 1, "gene": null, "product": "HTH rpiR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0", "UniRef:UniRef90_C4XDS0" ], "nt": "TTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAATTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCTAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATGAATTCGATCCATGATTTTAAAGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGACAAAAGCAATTTTAATTTTCAAATATTGACAAGAAATGAAAATGTTTCTTATGCAAATTGCACCTCACCGATAATTTATGATTATATTCCAGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTTATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAA", "aa": "MQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCMNSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK", "aa_hexdigest": "956c9193bb14e28c4086775a3e068e09", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 548644, "stop": 549447, "observations": { "mutation": [ 548719 ], "stop": [ 548719 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS0", "query_cov": 1.0, "subject_cov": 0.7490636704119851, "identity": 0.99, "score": 388.0, "evalue": 2.89e-135, "gene_start": 1, "gene_end": 200, "reference_start": 68, "reference_end": 267, "reference_sequence": "MMKRIKIFDKKLVENYKDTYSYLIKWIEENPHEFLACSQHQITEKIYVSQSSLSRFTKFLGFESYRELQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCINSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK" }, "paralog": false, "identity": 0.9887640449438202, "score": 523.0, "evalue": 4.08e-191, "description": "Internal stop codon at 548719. Nonsense mutation around 548719.", "psc": { "uniref90_id": "UniRef90_C4XDS0", "product": "HTH rpiR-type domain-containing protein", "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS0", "UniRef:UniRef50_C4XDS0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0" ], "product": "HTH rpiR-type domain-containing protein" } }, "id": "IGIGPGHMMC_993", "locus": "GCA000148625_00967" }, { "type": "cds", "sequence": "contig_1", "start": 549600, "stop": 549761, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTGAATAATAATTACAACCGTAATTTTGCTTGA", "aa": "MKEKLVKYLKMTIVIFLAGSLIGIYFLKTGQFENHSQKILLDRLNNNYNRNFA", "aa_hexdigest": "7d8147d3f8fa390ecf5e923fe5acaa7c", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.3389, "isoelectric_point": 9.90029697418213 }, "id": "IGIGPGHMMC_994", "locus": "GCA000148625_00968" }, { "type": "cds", "sequence": "contig_1", "start": 549945, "stop": 550103, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGTTGATTTAGAAGACAACAAGAAAAAAATACAAAAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGATGGAATTTAA", "aa": "MYVPKVQKKLVIVDLEDNKKKIQKDNQKLKEAISFKKKEEVVSEQETYEDGI", "aa_hexdigest": "bcba13c02777634d7a0c8f5e74aacdde", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6153.060799999999, "isoelectric_point": 7.909384346008301 }, "id": "IGIGPGHMMC_995", "locus": "GCA000148625_00969" }, { "type": "cds", "sequence": "contig_1", "start": 550113, "stop": 550685, "strand": "+", "frame": 3, "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "db_xrefs": [ "EC:5.3.1.17", "GO:0008697", "GO:0045490", "KEGG:K01815", "SO:0001217", "UniRef:UniRef50_A0A449A2L5", "UniRef:UniRef90_C4XDS2" ], "nt": "ATGAGATTTAAAACAACATATGCAGTTTCAGCAAACGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGTGGAAAACTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACACCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTTTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTCAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGTCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAGGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGCTTACAATTTTAGAACCTAACAATATGTGA", "aa": "MRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGLTILEPNNM", "aa_hexdigest": "28b78351f25c7f444add990abbaa8553", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "kduI" ], "truncated": "3-prime", "pseudogene": { "start": 550110, "stop": 550952, "observations": { "mutation": [ 550683, 550839, 550884 ], "stop": [ 550683, 550839, 550884 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS2", "query_cov": 1.0, "subject_cov": 0.6785714285714286, "identity": 1.0, "score": 381.0, "evalue": 1.68e-132, "gene_start": 1, "gene_end": 190, "reference_start": 2, "reference_end": 191, "reference_sequence": "MMRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGLTILEPNNMWNTMPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAPWSIHSGVGTSNYAFIWAMGGENLDYTDMQGVKTIDLK" }, "paralog": false, "identity": 0.9821428571428571, "score": 554.0, "evalue": 2.96e-203, "description": "Internal stop codon at 550683, 550839, 550884. Nonsense mutation around 550683, 550839, 550884.", "psc": { "uniref90_id": "UniRef90_C4XDS2", "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "ec_ids": [ "5.3.1.17" ], "uniref50_id": "UniRef50_A0A449A2L5", "kegg_orthology_id": "K01815", "go_ids": [ "GO:0008697", "GO:0045490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS2", "EC:5.3.1.17", "UniRef:UniRef50_A0A449A2L5", "KEGG:K01815", "GO:0008697", "GO:0045490" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2L5" ], "product": "4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase" } }, "id": "IGIGPGHMMC_996", "locus": "GCA000148625_00970" }, { "type": "cds", "sequence": "contig_1", "start": 550692, "stop": 550841, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGCCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGA", "aa": "MPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAP", "aa_hexdigest": "e3d1cba795fd1407655ae716ebcf170c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5662.5078, "isoelectric_point": 6.891182136535646 }, "id": "IGIGPGHMMC_997", "locus": "GCA000148625_00971" }, { "type": "cds", "sequence": "contig_1", "start": 550952, "stop": 551149, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAGAAATCTAAATAATTCATTAGTCTATGAAAAAGAATTCAACGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGA", "aa": "MFRNLNNSLVYEKEFNDTKLLTKEEIKKALDLIIKQVDQNIDYFGKEFMIANTFNNKYQKIYSSE", "aa_hexdigest": "2ed10d89db86a72e78a44ed2b79c9e72", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7830.851400000001, "isoelectric_point": 6.243842887878418 }, "id": "IGIGPGHMMC_998", "locus": "GCA000148625_00972" }, { "type": "cds", "sequence": "contig_1", "start": 551288, "stop": 551425, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGA", "aa": "MGFLYSPSCVAAYKLTKNQEAKEAALLAADNLISRYQEKGKFIQA", "aa_hexdigest": "008699fd48050ab2f7dac701666d2cec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4981.7018, "isoelectric_point": 8.889368247985843 }, "id": "IGIGPGHMMC_999", "locus": "GCA000148625_00973" }, { "type": "cds", "sequence": "contig_1", "start": 551909, "stop": 552052, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGA", "aa": "MLKITQKDYKTDLYEKSAHLILRNLINKYHNKKIEPGTPILLHGVYS", "aa_hexdigest": "a2005db7a4beef07139611715a016d0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5539.4529999999995, "isoelectric_point": 9.573958015441892 }, "id": "IGIGPGHMMC_1000", "locus": "GCA000148625_00974" }, { "type": "cds", "sequence": "contig_1", "start": 552230, "stop": 552373, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGA", "aa": "MQVNGSNSVIVANDEAAVSETQQQLMKVTVPEGVRCSFYSVDKLIEI", "aa_hexdigest": "fdbc5a0ad85c6edb553bf231e3ff5b5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.764699999999, "isoelectric_point": 4.3588346481323255 }, "id": "IGIGPGHMMC_1001", "locus": "GCA000148625_00975" }, { "type": "cds", "sequence": "contig_1", "start": 552726, "stop": 552833, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGA", "aa": "MGTYTATPIVYALGVGIILGDIQTAVIVGAAGQTV", "aa_hexdigest": "850023f892d116746b7ff6b3a76fe1a3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3434.9936999999995, "isoelectric_point": 4.0500284194946286 }, "id": "IGIGPGHMMC_1002", "locus": "GCA000148625_00976" }, { "type": "cds", "sequence": "contig_1", "start": 552999, "stop": 553208, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGA", "aa": "MQLLTTLLFTLMSPISEIAKKQITKGKFKTFKFLSHVTILVLMIITFGFGLLVGVSANGIARFSSSIPD", "aa_hexdigest": "642d306918ad83035b34f00046d454a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7562.090899999997, "isoelectric_point": 10.294907951354979 }, "id": "IGIGPGHMMC_1003", "locus": "GCA000148625_00977" }, { "type": "cds", "sequence": "contig_1", "start": 553239, "stop": 553532, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAA", "aa": "MLPALGFALILKVMLKKEYIGFALLGYFLALVFEVISKITGQAFSVLALALTAMAFVLIIISIKKLAGIKEAAGVARNISNTSSNNENNKGGQQDGI", "aa_hexdigest": "dfcdad617cfb3ed09a556a990b2ab440", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10287.194299999986, "isoelectric_point": 9.631657218933103 }, "id": "IGIGPGHMMC_1004", "locus": "GCA000148625_00978" }, { "type": "cds", "sequence": "contig_1", "start": 553849, "stop": 554271, "strand": "+", "frame": 1, "gene": null, "product": "PTS system mannose/fructose/sorbose family IID component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7", "UniRef:UniRef90_C4XDS6" ], "nt": "ATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATCGGTTTTGCTCAAAGCGGAAATATACTGGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATTAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTCTAGACAACATTATGCCACTATTTGCTTCAGGAATATGA", "aa": "MSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGI", "aa_hexdigest": "6bb174903953e55ef2f94aae8d55bd27", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 553549, "stop": 554385, "observations": { "mutation": [ 553585, 553825, 554269, 554308 ], "stop": [ 553585, 553825, 554269, 554308 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS6", "query_cov": 1.0, "subject_cov": 0.4713804713804714, "identity": 1.0, "score": 255.0, "evalue": 2.11e-83, "gene_start": 1, "gene_end": 140, "reference_start": 120, "reference_end": 259, "reference_sequence": "MMKITREVNKMESNNKDFVQVNQAQTKKLSIWTYIIVALRSYFLQSGFNYANFQGLGYANIIYPALKKIYGYGTQQFKDALLNNIEFYNTNPQCIPLITSIHIQLLNNGTSWADARTIKMSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGIWVIFIYFMVTKYKWSPYKVIVVTILVGVTAGVFGILGV" }, "paralog": false, "identity": 0.9856115107913669, "score": 520.0, "evalue": 2.82e-190, "description": "Internal stop codon at 553585, 553825, 554269, 554308. Nonsense mutation around 553585, 553825, 554269, 554308.", "psc": { "uniref90_id": "UniRef90_C4XDS6", "product": "PTS system mannose/fructose/sorbose family IID component", "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS6", "UniRef:UniRef50_A0A449A2J7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7" ], "product": "PTS system mannose-specific EIID component" } }, "id": "IGIGPGHMMC_1005", "locus": "GCA000148625_00979" }, { "type": "cds", "sequence": "contig_1", "start": 554385, "stop": 554792, "strand": "+", "frame": 3, "gene": null, "product": "PTS EIIA type-4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354709.1", "SO:0001217", "UniParc:UPI0001E32E8F", "UniRef:UniRef100_A0AB32XCB1", "UniRef:UniRef50_C4XDS7", "UniRef:UniRef90_C4XDS7" ], "nt": "ATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGGTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAA", "aa": "METKILVIGHSQFPRGVVEGLKFVSGIEEDIDYKYIDNDHSIDELKNDLEKYLEKCENIIIFADLPGGSPHRLAVETIEKTKKKNAIVIAGAGMPMIMDIAFKVILIKFKSFDEIICFIGEKKKNLDKYIMITGA", "aa_hexdigest": "61f2c5627429de0f7932c21002b0d7d5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32E8F", "ncbi_nrp_id": "WP_013354709.1", "uniref100_id": "UniRef100_A0AB32XCB1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E8F", "RefSeq:WP_013354709.1", "UniRef:UniRef100_A0AB32XCB1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCB1", "uniref90_id": "UniRef90_C4XDS7" }, "psc": { "uniref90_id": "UniRef90_C4XDS7", "product": "PTS EIIA type-4 domain-containing protein", "uniref50_id": "UniRef50_C4XDS7" }, "pscc": { "uniref50_id": "UniRef50_C4XDS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS7" ], "product": "PTS EIIA type-4 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1006", "locus": "GCA000148625_00980" }, { "type": "cds", "sequence": "contig_1", "start": 554795, "stop": 555130, "strand": "+", "frame": 2, "gene": "yajC", "product": "Preprotein translocase subunit YajC", "db_xrefs": [ "KEGG:K03210", "RefSeq:WP_013354710.1", "SO:0001217", "UniParc:UPI0001E32E90", "UniRef:UniRef100_C4XDS8", "UniRef:UniRef50_C4XDS8", "UniRef:UniRef90_C4XDS8" ], "nt": "ATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGATGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAA", "aa": "MDLAVKIVLFILLGLFILQIIVVTVILPILKKKRDEKIAREHKELVKNIKSGHEVLLQNGIYGTYVNSSGDETFNMRINEENNVIIKVNKSAILGFFDKDIAKKIEQRKQK", "aa_hexdigest": "b6902950ec64bb2a671c1fd0db03b859", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32E90", "ncbi_nrp_id": "WP_013354710.1", "uniref100_id": "UniRef100_C4XDS8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E90", "RefSeq:WP_013354710.1", "UniRef:UniRef100_C4XDS8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDS8", "uniref90_id": "UniRef90_C4XDS8" }, "psc": { "uniref90_id": "UniRef90_C4XDS8", "product": "Preprotein translocase subunit YajC", "uniref50_id": "UniRef50_C4XDS8", "kegg_orthology_id": "K03210" }, "pscc": { "uniref50_id": "UniRef50_C4XDS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS8" ], "product": "Preprotein translocase subunit YajC" }, "genes": [ "yajC" ], "id": "IGIGPGHMMC_1007", "locus": "GCA000148625_00981" }, { "type": "cds", "sequence": "contig_1", "start": 555143, "stop": 555484, "strand": "+", "frame": 2, "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "db_xrefs": [ "EC:1.1.1.127", "SO:0001217", "UniRef:UniRef50_A0A975S3K9", "UniRef:UniRef90_C4XDS9" ], "nt": "ATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAAGACAAAGTTGTTATTGTTACAGGTGCAAACATGGGATTAGGCTTAGCTTATGCTAAAGCTTTATCAAATTGTGGTGCCAAATTAGTTATTAGTCATTTTGACACAAATACGAAAGAAATAGAAAACTTTTGTGCTAAAAATAAAACAGAACTATTGCTTGTTCAAGGTGACTTAACTAAAGAAACAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGTACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGA", "aa": "MDFKFNKDYFSVKDKVVIVTGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKETDRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKS", "aa_hexdigest": "1ea07ec79bfa29e3a608262a3ea96bfa", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "kduD" ], "truncated": "3-prime", "pseudogene": { "start": 555143, "stop": 555787, "observations": { "mutation": [ 555482, 555518 ], "stop": [ 555482, 555518 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS9", "query_cov": 1.0, "subject_cov": 0.4396887159533074, "identity": 0.982, "score": 222.0, "evalue": 1.58e-71, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MDFKFNKDYFSVKDKVVIITGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKEADRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKSWNKVIDINMNAVWFLSQKVAQIMKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGHWAKPEEIAGAVVFLCSKASDYISGAIIPVDGGFLCR" }, "paralog": false, "identity": 0.9813084112149533, "score": 407.0, "evalue": 6.6e-147, "description": "Internal stop codon at 555482, 555518. Nonsense mutation around 555482, 555518.", "psc": { "uniref90_id": "UniRef90_C4XDS9", "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "ec_ids": [ "1.1.1.127" ], "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS9", "EC:1.1.1.127", "UniRef:UniRef50_A0A975S3K9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A975S3K9" ], "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD" } }, "id": "IGIGPGHMMC_1008", "locus": "GCA000148625_00982" }, { "type": "cds", "sequence": "contig_1", "start": 555548, "stop": 555808, "strand": "+", "frame": 2, "gene": null, "product": "2-keto-3-deoxygluconate oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "nt": "ATGAAAAAACAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGA", "aa": "MKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGH", "aa_hexdigest": "c17948bc9b73689f5eed682bff6a069d", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0015F461D0", "query_cov": 0.8488372093023255, "subject_cov": 0.9733333333333334, "identity": 0.589, "score": 88.6, "evalue": 1.76e-21, "valid": false, "product": "SDR family NAD(P)-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A377BY30" }, "pscc": { "uniref50_id": "UniRef50_A0A377BY30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "product": "2-keto-3-deoxygluconate oxidoreductase" }, "genes": [], "id": "IGIGPGHMMC_1009", "locus": "GCA000148625_00983" }, { "type": "cds", "sequence": "contig_1", "start": 555907, "stop": 556404, "strand": "+", "frame": 1, "gene": null, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "db_xrefs": [ "EC:4.1.2.14", "EC:4.1.3.42", "KEGG:K01625", "SO:0001217", "UniRef:UniRef50_D5E5I3", "UniRef:UniRef90_C4XDT0" ], "nt": "ATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGA", "aa": "MSLSALDLIKKEKLIAVIRYNSYEEAKKAIETCIKGGIKIIEITLTTPEALKLIKEFSKSKVLIGAGTVVSHEDAYKSVKSGAKFLVSPHFSDSVNEVSQISEIPYLAGVMTPTEAISAYRQGSKLLKVFPSEIVGPNFISGLLKPFPFLELMPSGGVNLDNAAA", "aa_hexdigest": "1b6e50eb9980b0490f624c012d0f7917", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_C4XDT0", "query_cov": 1.0, "subject_cov": 0.8168316831683168, "identity": 1.0, "score": 316.0, "evalue": 1.55e-108, "valid": true, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "ec_ids": [ "4.1.2.14", "4.1.3.42" ], "uniref50_id": "UniRef50_D5E5I3", "kegg_orthology_id": "K01625" }, "pscc": { "uniref50_id": "UniRef50_D5E5I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5I3" ], "product": "Khg/kdpg aldolase" }, "genes": [], "id": "IGIGPGHMMC_1010", "locus": "GCA000148625_00984" }, { "type": "cds", "sequence": "contig_1", "start": 556508, "stop": 557020, "strand": "+", "frame": 2, "gene": "pfkB", "product": "Carbohydrate kinase PfkB domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1", "UniRef:UniRef90_C4XDT1" ], "nt": "ATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGA", "aa": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTL", "aa_hexdigest": "8f957613d390c60272f158a2246db4d6", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkB" ], "truncated": "3-prime", "pseudogene": { "start": 556508, "stop": 557323, "observations": { "mutation": [ 557018, 557090 ], "stop": [ 557018, 557090 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT1", "query_cov": 1.0, "subject_cov": 0.5167173252279635, "identity": 1.0, "score": 332.0, "evalue": 8.45e-113, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTLWNNKYADFKKEIDPFLNLSDIVFGWISEQNYCKSNSSISAKDFDKLTCLIDKKYKNIELIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFEIER" }, "paralog": false, "identity": 0.992619926199262, "score": 531.0, "evalue": 1.13e-193, "description": "Internal stop codon at 557018, 557090. Nonsense mutation around 557018, 557090.", "psc": { "uniref90_id": "UniRef90_C4XDT1", "product": "Carbohydrate kinase PfkB domain-containing protein", "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT1", "UniRef:UniRef50_C4XDT1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1" ], "product": "Carbohydrate kinase PfkB domain-containing protein" } }, "id": "IGIGPGHMMC_1011", "locus": "GCA000148625_00985" }, { "type": "cds", "sequence": "contig_1", "start": 557192, "stop": 557497, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00294.29" ], "nt": "TTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTGAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAACTTGAAAGGTAA", "aa": "MIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFELER", "aa_hexdigest": "219ac7e742b71f3a532614327ce8f539", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00294.29", "name": "PfkB", "length": 51, "aa_cov": 0.49504950495049505, "hmm_cov": 1.0, "evalue": 8.917301620981438e-09, "score": 31.065217971801758, "start": 21, "stop": 70 } ], "seq_stats": { "molecular_weight": 11662.07439999999, "isoelectric_point": 6.231167793273925 }, "id": "IGIGPGHMMC_1012", "locus": "GCA000148625_00986" }, { "type": "cds", "sequence": "contig_1", "start": 557500, "stop": 558510, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATAGACCATCCTCTTGCTAACTTATATTATGCTGGAACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAATAAAAGCTAAAAAAACTTTTGATCGAGAGTTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGCCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCCGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGAGAAATTAAAAAATATTATTGAGAATGTTTTAGACGATTTTGCAAAAAATTATTATTACAACGGACAACAACAATATGTTAACTGA", "aa": "MKLKKIILPLSMSAAIMPALSSSCTNLKSEGFDKYGNRIPSEINQLKNYLIFKERKVAIENTKASVAANAINNVENEDRYNQILSFLEPEFEILPKNFEYQYSAKVINKSEGDLLEINILVIQTSTGSKENIKLTLGQDTKFKKDSNLSKSDYEQILKNILIVNDLKYLVEEDESKLSDNDKKNKNNAVRFTDIILNKYNENLFIDHPLANLYYAGTYNNGTGIKATDSNEIKAKKTFDRELKAIEEAKELIYRKESQPVQDPETKRIVDEGYSDAQKVRVAIQKLLFLAAAYQIKPKGIFTNKFYQNEKLKNIIENVLDDFAKNYYYNGQQQYVN", "aa_hexdigest": "cfd99311ee7159a52d10dd36ca58d6a6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38612.41890000002, "isoelectric_point": 8.199040794372557 }, "id": "IGIGPGHMMC_1013", "locus": "GCA000148625_00987" }, { "type": "cds", "sequence": "contig_1", "start": 558622, "stop": 559632, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08124.17" ], "nt": "TTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAACCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGATGGTTTTTATGTTGACGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTTCTGTATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCTTAGAAATAAGGAAGATTGGGAATTTTATGAAAATAATTATTTTGATCAAAGAAAAGAACCAAGTTCAACAAACGAGCATTCAGAAAGCCCAGACAATGCTTTAGTCGGTGTTGATTTACATAAAGAAAACGATGGTTTAGTATTTACAAAAAATCAAGATAGATATGTTTACAAACATGATGATTTTATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTCCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGA", "aa": "MPNPRYGGAAPTAILAYKPVTKKRAQTGANAVDNAKPILISGILNEDTSKIEDCILSLYDNIFRDFVKKGDGFYVDGSYMQHTNIPYVGSYGEVLFTNIADIFKYFENTDLALGKDKRFEKIYQFIELSIMPFMFKGAISDGLSGRSIVRDDHSDLKKGLNILGSLAIIEKSASSVYKSRLREFIKNQISHLNKTQLQKLASEHKVRPLLIDALLEIKDLPDLPHPLRNKEDWEFYENNYFDQRKEPSSTNEHSESPDNALVGVDLHKENDGLVFTKNQDRYVYKHDDFMFNINLHGRISGFPEASVQENMDSYYQYDGSTFLATNNNYSPYSNKY", "aa_hexdigest": "22c0d8f02b88b030f1424d67d83a0b10", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08124.17", "name": "Lyase_8_N", "length": 169, "aa_cov": 0.5029761904761905, "hmm_cov": 0.9822485207100592, "evalue": 2.151306690358416e-40, "score": 134.75218200683594, "start": 18, "stop": 186 } ], "seq_stats": { "molecular_weight": 38366.67370000005, "isoelectric_point": 5.882574272155761 }, "id": "IGIGPGHMMC_1014", "locus": "GCA000148625_00988" }, { "type": "cds", "sequence": "contig_1", "start": 559957, "stop": 560181, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACAATTGAAAATAGAATGATCATTGATAAAAGCACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAGAGGAACAAGATTTATTTATTTGA", "aa": "MGFVKAEVINYNNTLITDKVYFMVNGIIIVIGNTNIKNKLVNTTIENRMIIDKSTDMFSNQKVNINGEEQDLFI", "aa_hexdigest": "0b2aacd5567cb0d90028fc97863fdeff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8456.697699999997, "isoelectric_point": 5.135198402404785 }, "id": "IGIGPGHMMC_1015", "locus": "GCA000148625_00989" }, { "type": "cds", "sequence": "contig_1", "start": 560248, "stop": 560688, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGATAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTTATAGCGTTAAAATATCAAAATAAAGACTCTTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATACGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCCGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGACTTAGATCTAAAAATTAATTTATGA", "aa": "MHEAKYPIITNMNSKKMVPVVRDFASLSINHEQNNTFMYAIVPNYEDSKKSNYLDILNNIKIIKNDQNFIALKYQNKDSLGQINDYYFVASLYENGNIKYKEQLNSLGSYDNEVSINYNEIPEFERNYKAYKNGLYLEDLDLKINL", "aa_hexdigest": "20624892c748cbec32c183ed6ffe1637", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17188.1405, "isoelectric_point": 5.3910306930542 }, "id": "IGIGPGHMMC_1016", "locus": "GCA000148625_00990" }, { "type": "cds", "sequence": "contig_1", "start": 560701, "stop": 560955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATCAAGAAAGTTCAAAATTCTTCAATCTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTGTCAATTTAAACGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGA", "aa": "MIIKKVQNSSILDVLVSNDLNENQYEIEFSKAFQNITSRKITNKKFSAKNVVNLNEVTLNNVLGQSKIRVDNRLGEVNDYNHIS", "aa_hexdigest": "dee4a8ed199badf6c3fdc4dca5220d17", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9609.758199999997, "isoelectric_point": 9.048927497863769 }, "id": "IGIGPGHMMC_1017", "locus": "GCA000148625_00991" }, { "type": "cds", "sequence": "contig_1", "start": 560984, "stop": 561094, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATAAAATTATATTTGCTTTAACTCCGTTCACTTTCACGCCTTTAACCGCAATAAGTTGTAGTATTGAAAGTTCAAATGATTATGAAAACGCTAATAAATGA", "aa": "MKKHKIIFALTPFTFTPLTAISCSIESSNDYENANK", "aa_hexdigest": "8a0a3fdb2a27db13311aa748f91d5f28", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4061.635000000001, "isoelectric_point": 7.922987174987794 }, "id": "IGIGPGHMMC_1018", "locus": "GCA000148625_00992" }, { "type": "cds", "sequence": "contig_1", "start": 561119, "stop": 561355, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGCGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTAGTTTAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTCAGATGAAACAACTACAAATTTTGACCAAAATTCCTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGA", "aa": "MKESENILINKLIKSEFKITNFYNEMILNKEFNINEVSLKITYNKNFKAFNNSDETTTNFDQNSFKTGKIVNLKKYFT", "aa_hexdigest": "9b8c765a29124b221688b10af12577bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9285.477099999993, "isoelectric_point": 9.188759422302244 }, "id": "IGIGPGHMMC_1019", "locus": "GCA000148625_00993" }, { "type": "cds", "sequence": "contig_1", "start": 561398, "stop": 561607, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAGCTAACATTATACAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAG", "aa": "MQSYFNPLSSKFADYVTEDTLYLKLTLYNSCALNLKTQHIIYAFTNSNKTGRLITKDNTLELQKLTQIS", "aa_hexdigest": "0a8e60c4f66b5a01b1982d28abd92662", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7969.017099999999, "isoelectric_point": 8.788346290588382 }, "id": "IGIGPGHMMC_1020", "locus": "GCA000148625_00994" }, { "type": "cds", "sequence": "contig_1", "start": 561622, "stop": 561984, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATTCTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGA", "aa": "MKKIKMILTLSSLTTCLPIIATSCKNEKEEKITNFDEVKSKLTFDVDNKSNIKASEFKDTNLIKINNLPSDFKKEITLENYEDGKIKFKLILIDKNNKKSQAYSITISGFKAETATPTIN", "aa_hexdigest": "7964adbfba9715c42bcde2218ae7c995", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 85, "aa_cov": 0.6416666666666667, "hmm_cov": 0.9647058823529412, "evalue": 5.097317999051525e-08, "score": 29.20128631591797, "start": 35, "stop": 111 } ], "seq_stats": { "molecular_weight": 13610.655699999978, "isoelectric_point": 9.148466682434083 }, "id": "IGIGPGHMMC_1021", "locus": "GCA000148625_00995" }, { "type": "cds", "sequence": "contig_1", "start": 562156, "stop": 562434, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGA", "aa": "MTKDTSNSPVYTFEISNFEFTEEFDFVNFDEVASKYKELKLLTANKAEAKNVIAAYNATKSSSLDAKIKDNILVLMAGIFAKGKNSSKPQAS", "aa_hexdigest": "43177eb6ec6e342c5437a931d5148af9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10166.370999999994, "isoelectric_point": 6.232304573059082 }, "id": "IGIGPGHMMC_1022", "locus": "GCA000148625_00996" }, { "type": "cds", "sequence": "contig_1", "start": 562669, "stop": 562860, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGA", "aa": "MLELIARTFATLSDTQKTANKTKLDILYNATKIFLPKASNLNSFKGEDANLSDGKNYKPTFNR", "aa_hexdigest": "1b942679f5eb2234a6ad148529c40b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7082.998200000002, "isoelectric_point": 9.630754661560058 }, "id": "IGIGPGHMMC_1023", "locus": "GCA000148625_00997" }, { "type": "cds", "sequence": "contig_1", "start": 563884, "stop": 564546, "strand": "+", "frame": 1, "gene": null, "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "EC:6.3.4.21", "KEGG:K00763", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_C4XDT5" ], "nt": "ATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGA", "aa": "MTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKL", "aa_hexdigest": "e0c4b595eac16f195cfadef4cc951529", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 563866, "stop": 564849, "observations": { "mutation": [ 564544 ], "stop": [ 564544 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT5", "query_cov": 1.0, "subject_cov": 0.6358381502890174, "identity": 1.0, "score": 435.0, "evalue": 2.8e-152, "gene_start": 1, "gene_end": 220, "reference_start": 7, "reference_end": 226, "reference_sequence": "MIRMVQMTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKLWGVRIDTSKNMVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKRK" }, "paralog": false, "identity": 0.9938837920489296, "score": 639.0, "evalue": 4.91e-235, "description": "Internal stop codon at 564544. Nonsense mutation around 564544.", "psc": { "uniref90_id": "UniRef90_C4XDT5", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "kegg_orthology_id": "K00763", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT5", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "KEGG:K00763" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "IGIGPGHMMC_1024", "locus": "GCA000148625_00998" }, { "type": "cds", "sequence": "contig_1", "start": 564574, "stop": 564906, "strand": "+", "frame": 1, "gene": null, "product": "Nicotinate phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3", "UniRef:UniRef90_A0A3P8LBM3" ], "nt": "ATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAGAAAATAA", "aa": "MVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKRK", "aa_hexdigest": "a57741ad51398ee1c19539f8136b4dc4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 564478, "stop": 565062, "observations": { "insertion": [ 564838 ], "deletion": [ 565034 ], "mutation": [ 564544 ], "stop": [ 564544, 564881, 564917, 564938 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBM3", "query_cov": 0.8272727272727273, "subject_cov": 0.52, "identity": 0.835, "score": 150.0, "evalue": 2.78e-44, "gene_start": 1, "gene_end": 91, "reference_start": 33, "reference_end": 123, "reference_sequence": "MDYHNNVIEQALESYKVLGNKLWGVRIDTSKNMVDHMFDNQEENPEHYGVNIEQVKNLRSALDKVGANDVKIVVSSGFNAAKIKKFEEADTPVDSYGVGAGIFKFVSSFSADAVLLNGKKKQKRVANIDLIQNCKFTILKNNQFSKTPTKQSFWKIFIINNTLQKISKFKKLSII" }, "paralog": false, "identity": 0.6649484536082474, "score": 217.0, "evalue": 3.42e-73, "description": "Internal stop codon at 564544, 564881, 564917, 564938. Frameshift due to insertion around 564838. Frameshift due to deletion around 565034. Nonsense mutation around 564544.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBM3", "product": "Nicotinate phosphoribosyltransferase", "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBM3", "UniRef:UniRef50_A0A3P8LBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3" ], "product": "Nicotinate phosphoribosyltransferase" } }, "id": "IGIGPGHMMC_1025", "locus": "GCA000148625_00999" }, { "type": "cds", "sequence": "contig_1", "start": 565075, "stop": 565350, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGTGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTGAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY", "aa_hexdigest": "25f9e36794dd6b75a6d134d610038f68", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8053097345132744, "identity": 0.9890000000000001, "score": 174.0, "evalue": 4.75e-55, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10689.122099999988, "isoelectric_point": 7.929176139831544 }, "id": "IGIGPGHMMC_1026", "locus": "GCA000148625_01000" }, { "type": "cds", "sequence": "contig_1", "start": 565546, "stop": 566292, "strand": "-", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAACAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTGTTAACTTAATCAAAAATATGATTAATCAATTAATTGATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTGTAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAGGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCTAGACTTGCAAGCAAATACAATGTAAGCAAAACTCAAATATACAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAGAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACCTTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACAATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCCAGCAAATGTTTATCAAAAACAATTAAAGAAGCAATAACAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAVNLIKNMINQLIDYKYSDERKSKYKAHDVIVRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRLASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISSKCLSKTIKEAITKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "b350ac9d7d217e4ba9aaeb16a0727721", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 565243, "stop": 566367, "observations": { "mutation": [ 565404, 565548 ], "stop": [ 565404, 565548 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 0.976, "score": 477.0, "evalue": 3.02e-167, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9679144385026738, "score": 725.0, "evalue": 1.84e-267, "description": "Internal stop codon at 565404, 565548. Nonsense mutation around 565404, 565548.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "IGIGPGHMMC_1027", "locus": "GCA000148625_01001" }, { "type": "cds", "sequence": "contig_1", "start": 566632, "stop": 566853, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGATAATGGCGTTGACTTTCAGATAGTTGTTAAAAATTTAAAAGCAACTAAACCATATGACTTAAATAAACTAGAAATTGATACTGTTACAACTAGTGAAGAAAACAAGGAAAAACTTATTGATTCTGTTTATAAAATAATAGAAAAAAATACTCTTATTCCAAAAGAAGAATTAAAAAACAAGACAGAATTAATTGTTGAAGATGGTAATTGA", "aa": "MQDDNGVDFQIVVKNLKATKPYDLNKLEIDTVTTSEENKEKLIDSVYKIIEKNTLIPKEELKNKTELIVEDGN", "aa_hexdigest": "f43229693a07c356eab0d171593ac7b6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8394.422499999995, "isoelectric_point": 4.644280052185059 }, "id": "IGIGPGHMMC_1028", "locus": "GCA000148625_01002" }, { "type": "cds", "sequence": "contig_1", "start": 566926, "stop": 567045, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAAATTTTGAAAAAATTAAGAAAGAAGCAAAAGAACAAATTACATATAAAATTAAAGAAGCTTTTGGCCCAAATTCAAAGCTTGTACAAAATAATTTATATGATTTTTTATGA", "aa": "MDKNFEKIKKEAKEQITYKIKEAFGPNSKLVQNNLYDFL", "aa_hexdigest": "be59a369bf074fa0f8ff0263594f7270", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4664.337200000002, "isoelectric_point": 9.050087928771973 }, "id": "IGIGPGHMMC_1029", "locus": "GCA000148625_01003" }, { "type": "cds", "sequence": "contig_1", "start": 567289, "stop": 567759, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAATTTAACGATGATGTTGAAACTATAAACAACAATAAGACAAAGACAATAAACGAATTTGAAGATATTATAAAACCATTAACTGTCGACAAATATGTTAAATCTCTTAAAAATTCAGAAACGGGTGAAGATATCAAAGATATTAATAAAATAATTAAGTATAGATATATTATAGATACCCATAAAAAACTAAAAATTAATATTAGTATGTTAAATAATGAATATTATATAAGTAATTTCTCAGAAAAAGAGTATGACGTTGATTTAGGAGCATTTGACGAAGCATATTTGTCTGACTTTGAACCACCTGTTATTAATCTTTATGGCCGGAATACGATTGATTTAATTAAAAATTATCTTAAAAACACAAGCGAGTATGGTGTCTTACAAAGTTATTTAAATGAATATAATCGCAAAAAACTATCTGAAAACAACAAACTTTTTTCAAACAAAAAAACTAAATGA", "aa": "MLEFNDDVETINNNKTKTINEFEDIIKPLTVDKYVKSLKNSETGEDIKDINKIIKYRYIIDTHKKLKINISMLNNEYYISNFSEKEYDVDLGAFDEAYLSDFEPPVINLYGRNTIDLIKNYLKNTSEYGVLQSYLNEYNRKKLSENNKLFSNKKTK", "aa_hexdigest": "13ef4f0c78f0aac1c60c2769db209188", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18449.597900000004, "isoelectric_point": 5.579054069519042 }, "id": "IGIGPGHMMC_1030", "locus": "GCA000148625_01004" }, { "type": "cds", "sequence": "contig_1", "start": 567862, "stop": 568080, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATAGATATAAGTTTGCTACATTTAATATAAATATAAAACTAGATATTCTAAAACTAAAGAATGATTTTAAACCTGATAAAAATAAGAGTGCTAATGAAAAACTTCTCAATCATTTAGAAGCTTCTAATTTTGATGAACAAGATTTTTTAAATAAATATGTTAAAGCGACATCTAAAATAGATAAAAATGATCTAAAAATTAAAAATAAATGA", "aa": "MFYRYKFATFNINIKLDILKLKNDFKPDKNKSANEKLLNHLEASNFDEQDFLNKYVKATSKIDKNDLKIKNK", "aa_hexdigest": "4763359dd0d0d365f5b1220e5cfb01f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8614.838499999994, "isoelectric_point": 9.584917640686033 }, "id": "IGIGPGHMMC_1031", "locus": "GCA000148625_01005" }, { "type": "cds", "sequence": "contig_1", "start": 568759, "stop": 569121, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCTTTTCTTTCATCTTGTTTCAGACATGAATATAAACCTTTTTTTATAAGTATTTCTGGATCTTCGCAAGGTGTATATGTTAGTTTTGATTCTTACGATTATTCAGAATTCTTGCATGAATTATTGCATGAATTATCAAAATATATTCTTTACTATTATTATTATAGAATTGTTGATTTTGAATATTACGATAATAAAAAATTCTTTGAGGATAGTCTTGGTTTGCACCCAGAACTATATAAAGAAATGGAGAAAGAAGGTGGCCCTCTTTACCATATATTTTTCTTAAAAGAATTTATAAATTTTGGAATTAAATCCTTTGAAATGGAAGACTACAAAAAAGTTAAAAAATTTTGA", "aa": "MYSFLSSCFRHEYKPFFISISGSSQGVYVSFDSYDYSEFLHELLHELSKYILYYYYYRIVDFEYYDNKKFFEDSLGLHPELYKEMEKEGGPLYHIFFLKEFINFGIKSFEMEDYKKVKKF", "aa_hexdigest": "8d5e4d557a14171c49479a1166bc0a7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14793.61979999999, "isoelectric_point": 5.5009572982788075 }, "id": "IGIGPGHMMC_1032", "locus": "GCA000148625_01006" }, { "type": "cds", "sequence": "contig_1", "start": 569236, "stop": 569520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGGGAAGATATTAAGTTTGACCAAGATTACATAAAGAAAAAAATTAATATGCTTTTTGAATTATTATTTTGTAATACTCCTACACCTTACTCAGACAAAAGCTCTTTACACACCCCAGGAATTGATAGTGTATGGGAATATATAATAAAAAAAGGTTTTGAGAATAAAAAAGCATTTGAAAATTTTTTGCCTTTATATAACAATTTTTATAATAGTCTTTTTTCTAATGGGACAAAAGTAGACAAATATTTATCTGATAATTCGTTTAATATTCATTGA", "aa": "MKREDIKFDQDYIKKKINMLFELLFCNTPTPYSDKSSLHTPGIDSVWEYIIKKGFENKKAFENFLPLYNNFYNSLFSNGTKVDKYLSDNSFNIH", "aa_hexdigest": "8b9036389de057ce70b560c15a3556b4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11189.58499999999, "isoelectric_point": 7.797144889831544 }, "id": "IGIGPGHMMC_1033", "locus": "GCA000148625_01007" }, { "type": "cds", "sequence": "contig_1", "start": 570559, "stop": 570708, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACACAAAAAAATATGTCTTTTAGCAGTGCTTAGTGGGTTTGTCCCATTAAGTACTATTTTCATTTCAAACTCTGTTAATACACAAAATAATAATGTTAATAAAGAAACTAATATAACAAAGAAAATCACTGTTAATTATTGA", "aa": "MKKHKKICLLAVLSGFVPLSTIFISNSVNTQNNNVNKETNITKKITVNY", "aa_hexdigest": "a17d7094551b0bbed4fd4bf72272158d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5507.430900000002, "isoelectric_point": 10.01917667388916 }, "id": "IGIGPGHMMC_1034", "locus": "GCA000148625_01008" }, { "type": "cds", "sequence": "contig_1", "start": 570899, "stop": 571732, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGCAAAGGACTTGAAACCAAAATAAATCCTTTACAAGTTAACTTTGAAAGTGAAAAGGATAATGTTGCTGTTACAAACAATATGTTAATAACTAACCATATTAAGAAAACTAAAGAATTCTTTTCATTATTGTATCCTGACTTAGATGAAAAGGATTTAAGAGTTGTGACTTTCACACTATCAGAGTTTTATGCTAAGCAAAAATTCAAACCTATTGAAAAGATGAAAAATAATGAATTCCCAACAATGATTGATTATGTTAATTTCTTAGGTAAATATAAATTTAGCGATAAGAAGACAGAGTTATATTACAAAGATTCAATTAATAAACTATTTATAATCTTTAATCAAGATTTCTTAAATAATGGAATATTTAGTGCATTATATACTGGACATACAAATATTGATTTTGATAATTCATTTACTGTTATTGATACATATAACTTAATTAATAATATTTCTTCAAACGAAGGACAAGCATCATTATTCTTGATTCTTTCACACGTTAGAAATAGAATATCAAACAACTTCTTTATTAACAAAACTAAGAAGATTTGTTTAATTGTTGATGAAGCACATAGATTTATTAATGCTAAGAATATGTCTTCATTAGAATTCTTATTTGACACAGTTAAAACAATTCGTAAATACAACGGTTCTGTTATTTTAACTACACAAAACCCAAGTGACTTTGAATTAAGTGAAGACGTTATGAACCTTACAAAAGGTATGGTTGAAAATATACAATATACACTTATTTTAAGACAAAATCAAAATGGTGTTAATGCTATTAGTCAAATGTATAAATCTAAAAATGAGTTAACTTATTGA", "aa": "MGKGLETKINPLQVNFESEKDNVAVTNNMLITNHIKKTKEFFSLLYPDLDEKDLRVVTFTLSEFYAKQKFKPIEKMKNNEFPTMIDYVNFLGKYKFSDKKTELYYKDSINKLFIIFNQDFLNNGIFSALYTGHTNIDFDNSFTVIDTYNLINNISSNEGQASLFLILSHVRNRISNNFFINKTKKICLIVDEAHRFINAKNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSEDVMNLTKGMVENIQYTLILRQNQNGVNAISQMYKSKNELTY", "aa_hexdigest": "b408a42bc60f685a79f589a26806cb0e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 51, "aa_cov": 0.17689530685920576, "hmm_cov": 1.0, "evalue": 1.5026870414445887e-06, "score": 23.81978416442871, "start": 183, "stop": 231 } ], "seq_stats": { "molecular_weight": 32242.479000000025, "isoelectric_point": 8.359953880310062 }, "id": "IGIGPGHMMC_1035", "locus": "GCA000148625_01009" }, { "type": "cds", "sequence": "contig_1", "start": 572006, "stop": 572341, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAAATAATGCTAATGCAGACACAAAAAGTAATTTTAAAATTCAAAAAATTAGAGTTGAACAAGAAGTACTGGACGAACTAGTTAATTTTATTAACGTTCAAAAAATGAACTTATTTGATTCAAATTTAATTTTTGTTAATCAAAGTAAAGATTTGAAAACATTACACGAAATTGAAGTTCGGAATGGAATAAACGCTAAAAAAGCATTTGCCAATGTTTATTTAAATCACTATTTACAAAAAGAAGCTTTACAAAACTCAATATTTATCAAAGAAGACTTTTTGAGAAATGGCATTGAAATGAGCTCGCGTAATATTGTTCATGGTTGA", "aa": "MQLNNANADTKSNFKIQKIRVEQEVLDELVNFINVQKMNLFDSNLIFVNQSKDLKTLHEIEVRNGINAKKAFANVYLNHYLQKEALQNSIFIKEDFLRNGIEMSSRNIVHG", "aa_hexdigest": "7b34bd1891e7c9523cf221dff3146fed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12908.595499999985, "isoelectric_point": 8.005506706237792 }, "id": "IGIGPGHMMC_1036", "locus": "GCA000148625_01010" }, { "type": "cds", "sequence": "contig_1", "start": 572432, "stop": 573319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAATACCAAGTAAAAAACACAATTGCAGGCTCTATACGTTAATAGTTAGAATATTTAAATATAAATCCTCTACGAAGAAATATAAAAAGAATACACCAAATGATACAAAACTACTTGTACCATTTAAGAGAATAATTGAAAGTAAATATGTTGAAACATTATCATCAGCTGGAACAAGTATGTTTACTGTGTTTAAATTTAATGGTTTTAATATTTACTCAAACAACAGAGATGATAAATACGCATTTTTTAATCAATTAATTAATGTATTTAACTCAATTGAAACCAAATTCACATTTGTTAAAAAGCCAGCTTCTATGAATATAGAAGATAACTTAAAATACAATGTTGATAAGTATTTTGAAAGCAAAATGTTACAAAGCAACGAACTTGTTCAAAACCATTTTATTTACAATACACATGACTTTGAACAACTTAAAAAAATAGAAAACATTGATATTTATTATCTTGTTATTTATGGTAAGAATATAAATGAAATTAAACAACTAGAAAATGAGTTTATCAAGTCATTTGAAAATACCAAAATACCTTTATCAAATGTCACCGGCTATGAACTATTAGACTTTACAAATATGATTTACAATGTATCATTATCACAAAAACAAAAACAAAATTTCTTTGACGAAACACTTGATGAAGAGCAAACTATGATATTAGATGCTAAAATGAAAGGCAAAGAACTTTCAGACAAGGAATTAAAGAAATTACGTAAAGATAAAGAATATAAGCTTATGAACTTGCTTGCACCTGAAACAATAGAGTTCGGTTCAAAGCATATAACAATTAATAAAGGAGAAAATGAAAAATATTTATCATTTCAAACCTTATATGATTTTAGTTCTTTAAAAATTAATGAAGGTTGA", "aa": "MIIPSKKHNCRLYTLIVRIFKYKSSTKKYKKNTPNDTKLLVPFKRIIESKYVETLSSAGTSMFTVFKFNGFNIYSNNRDDKYAFFNQLINVFNSIETKFTFVKKPASMNIEDNLKYNVDKYFESKMLQSNELVQNHFIYNTHDFEQLKKIENIDIYYLVIYGKNINEIKQLENEFIKSFENTKIPLSNVTGYELLDFTNMIYNVSLSQKQKQNFFDETLDEEQTMILDAKMKGKELSDKELKKLRKDKEYKLMNLLAPETIEFGSKHITINKGENEKYLSFQTLYDFSSLKINEG", "aa_hexdigest": "2c2a221e1949d75638423ee0969f9ff9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34956.719699999994, "isoelectric_point": 9.027910804748537 }, "id": "IGIGPGHMMC_1037", "locus": "GCA000148625_01011" }, { "type": "cds", "sequence": "contig_1", "start": 573365, "stop": 573499, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAAATAAGAAAATAAGCTCATTAAAGCTTAATATCGTAAAGAATTTAAACCTTTATGATATTTTGTTGTTATTTGCAATAATAGGCTTTTCAGCAGCATTAGGATTTGCTAATAAACTTAACACTTGA", "aa": "MNQNKKISSLKLNIVKNLNLYDILLLFAIIGFSAALGFANKLNT", "aa_hexdigest": "685bbec0df066b04fb511772ff825a23", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4880.789600000001, "isoelectric_point": 10.000287437438963 }, "id": "IGIGPGHMMC_1038", "locus": "GCA000148625_01012" }, { "type": "cds", "sequence": "contig_1", "start": 573486, "stop": 573974, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACTTTGTTTCAAACGCAGTTAATAAAGTTAAAAACCTACACCCAGTACAAAAATTGAAGAACAAACTCAAAAAGAAAAATGCTGGTGCTGATGGAGGAGGAGATTCTGCTGATAATGAAAGTGAAGGTTCCTCTGATGGAAAAAGAAATGATGAAAATGCAAATACGAGTGATGCAACAGCTTATTCTGTTAAAGACGAGACCAATAAGAAAGCAATAAGCACTGAGAAAATTCCGCCAGCAGATATCCAGAAAACCAAAGAAAATATCGAAGGCGTAGCAGGAATAAGGGGAATACCTGATCTTTCAATTTATAAAAATGGGGATATTCAACAAAAATTAAGCGATATTTCTCTTTCATCAAAAGATATAGTGAAAAATCTTGCTGAAATGAAAAAAGCATCCCAAGAACTTGTAAAACATCAAGAAATAGATAACTATAAAGATAGCATAAAGGAGACAAAAAAACATGAATCAAAATAA", "aa": "MTNFVSNAVNKVKNLHPVQKLKNKLKKKNAGADGGGDSADNESEGSSDGKRNDENANTSDATAYSVKDETNKKAISTEKIPPADIQKTKENIEGVAGIRGIPDLSIYKNGDIQQKLSDISLSSKDIVKNLAEMKKASQELVKHQEIDNYKDSIKETKKHESK", "aa_hexdigest": "6ce59f52a32f737be36be2ad3648aacc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17711.520599999996, "isoelectric_point": 8.857778739929199 }, "id": "IGIGPGHMMC_1039", "locus": "GCA000148625_01013" }, { "type": "cds", "sequence": "contig_1", "start": 574065, "stop": 574724, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0396 family ICE element transmembrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FACEB8", "UniRef:UniRef90_UPI0002FACEB8" ], "nt": "ATGGCTTTTGCAGGGCTAGCACTTTTTGCTTTTGTTATGATTGCAATAGTCCTTGGTATTGCTTGTTTTAAAGTCCTTTTTGGTGGCGGCGGAGAAGACAAAATGCAAAAGATGAAGCAGAGTTTCAAAGCGGCATTACCACATATGGCTTTTATAATCTTAATTCCAATTGTTGTTTGGGTCCTTGCATTTTTTGCAAAATTTGCATTTAATATGCTAAATCTTGCTTTAGGGGCAGACCCAGCTGCAGGTTTAGGGGAAATAATTTATAAAGCGATAAATCAACCAGAGGCAAGATTTCCAAATCCAACAGAACCAACATATGGCCAATACTCTGCTTCACAAAATGAACTTAATAGAAATAGCGTTGTATTAATGATAAATGCATTAGTACTGATTTGGTCAATGATAGTTATTGCAAATATTGCTATAAATATAACAATCAACTCATATCAAGCATTCTTTTTATTTATACAATCACCATTAATAGCAGTAGTCTCAATTGAAGATGGTGGCAAGAAGCTTAATATATGGAAGCAACAATTTGTGTCCAAGATTCTTTCCATTATGGTCTTGAATTTATCATTGTCAGTACTGGTTATGTTTTTTTCAGCCGGCTCAAAAGTTATAATGCCTATGGCTAACGCAACTAAAGTATGA", "aa": "MAFAGLALFAFVMIAIVLGIACFKVLFGGGGEDKMQKMKQSFKAALPHMAFIILIPIVVWVLAFFAKFAFNMLNLALGADPAAGLGEIIYKAINQPEARFPNPTEPTYGQYSASQNELNRNSVVLMINALVLIWSMIVIANIAINITINSYQAFFLFIQSPLIAVVSIEDGGKKLNIWKQQFVSKILSIMVLNLSLSVLVMFFSAGSKVIMPMANATKV", "aa_hexdigest": "89241d9e0717836277ceaa4ddaff30c7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 573762, "stop": 574910, "observations": { "mutation": [ 574061, 574067, 574871 ], "stop": [ 574061, 574067, 574871 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002FACEB8", "query_cov": 1.0, "subject_cov": 0.4620253164556962, "identity": 0.973, "score": 397.0, "evalue": 2.26e-135, "gene_start": 1, "gene_end": 219, "reference_start": 63, "reference_end": 281, "reference_sequence": "MLKRIGFFFFKIGWFPFVAFPFTIINSLGKLINFLSFDFISLLIFGKSIDDIKSIDDIKSIPMAFAGLALFAFVMIAIILGIACFKVFFGGGGEDKMQKMKQSFKAALPHMAFIILIPIVVWVLAFFAKFAFNMLNLALGVDPAAGLGEIIYKAINQPEARFPNPTEPTYGQYSASQNELNRNSVVLMINALVLIWSMIVIANIAINISINSYQAFFLFIQSPLIAVVSIEDGGKKLNIWKQQFVSKILSVMVLNLSLSVLVMFFSAASKVIMPMANATKVWPWLIALIVFVAALYSTKSLLKIVCSIFGVEMTNFVSNAVNKVKSLHPVQKLKNKLKKKNAGADGGGDSADNESEGSSDGKSNDENANTSDATAYSVKDETNKKAISTEKIPPADIQKTKENIEGVAGIRGIPDLSIYKNGDIQQKLSDISLSSKDIVKNLAEMKKASQELVKHQEIDNYKDSIKETKKHESK" }, "paralog": false, "identity": 0.9659685863874345, "score": 637.0, "evalue": 2.13e-232, "description": "Internal stop codon at 574061, 574067, 574871. Nonsense mutation around 574061, 574067, 574871.", "psc": { "uniref90_id": "UniRef90_UPI0002FACEB8", "product": "Mbov_0396 family ICE element transmembrane protein", "uniref50_id": "UniRef50_UPI0002FACEB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002FACEB8", "UniRef:UniRef50_UPI0002FACEB8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FACEB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FACEB8" ], "product": "Mbov_0396 family ICE element transmembrane protein" } }, "id": "IGIGPGHMMC_1040", "locus": "GCA000148625_01014" }, { "type": "cds", "sequence": "contig_1", "start": 574729, "stop": 574944, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTATATCATCTATTGATTTAATATCATCTATTGATTTACCGAAAATTAATAATGATATAAAATCAAATGATAAAAAGTTAATCAACTTTCCTAAACTATTAATTATTGTGAATGGAAAAGCAACAAAAGGAAATCAACCAATTTTAAAAAAGAAAAATCCTATTCTTTTCAGCATATTTTATTATCCTTTAAGTGCTTCGCCACCATAA", "aa": "MLFISSIDLISSIDLPKINNDIKSNDKKLINFPKLLIIVNGKATKGNQPILKKKNPILFSIFYYPLSASPP", "aa_hexdigest": "8fb8a51efce2053147b654d6f6ef2ea4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7942.400599999997, "isoelectric_point": 9.854073143005369 }, "id": "IGIGPGHMMC_1041", "locus": "GCA000148625_01015" }, { "type": "cds", "sequence": "contig_1", "start": 574917, "stop": 575252, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_041593941.1", "SO:0001217", "UniParc:UPI0002F0BBCD", "UniRef:UniRef100_UPI0002F0BBCD", "UniRef:UniRef50_UPI0002F0BBCD", "UniRef:UniRef90_UPI0002F0BBCD" ], "nt": "ATGAATATTACTACAACAATGTTTCGCAATGTAGATACAAGTACAACAGCTGAAACAACATTTAAAAATATACAATATGCATTGATAGGCCAAGCTATTACTTGGTCAAGTATTATACTTGGCTCCTTATTTGCCTTAGCTGTTATTATTACTATTATAATCGTTAGTCATAAGGCAACTTTTGGCAAAAAAGATGATTCTTACCAAGAAAAATTAAAAGTTGTATTAAAAAGCTGTTTAATTGCAACTGCTGTACTTCTTGTGCTTCTTATTCTAACACCATCAATTTTAAAAATAATTCAAACTAATTATGGTGGCGAAGCACTTAAAGGATAA", "aa": "MNITTTMFRNVDTSTTAETTFKNIQYALIGQAITWSSIILGSLFALAVIITIIIVSHKATFGKKDDSYQEKLKVVLKSCLIATAVLLVLLILTPSILKIIQTNYGGEALKG", "aa_hexdigest": "4631d501df51eb2a42cec192b7841e2b", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002F0BBCD", "ncbi_nrp_id": "WP_041593941.1", "uniref100_id": "UniRef100_UPI0002F0BBCD", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F0BBCD", "RefSeq:WP_041593941.1", "UniRef:UniRef100_UPI0002F0BBCD" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F0BBCD", "uniref90_id": "UniRef90_UPI0002F0BBCD" }, "psc": { "uniref90_id": "UniRef90_UPI0002F0BBCD", "uniref50_id": "UniRef50_UPI0002F0BBCD" }, "pscc": { "uniref50_id": "UniRef50_UPI0002F0BBCD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F0BBCD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12076.224099999985, "isoelectric_point": 9.374750709533693 }, "id": "IGIGPGHMMC_1042", "locus": "GCA000148625_01016" }, { "type": "cds", "sequence": "contig_1", "start": 575624, "stop": 575794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGATGAAAAATATCCAACAGCCAACTATTATCTATTTAATGCTGATAATCCTGATTGGGACCACTCACAATCCAGATTTGTTACCGATATCTGCTACTATCTCAAAGAATTCTTATTACCGCTTGCTGCCTTAATCAAGCTAGAAATTAGTGATGAATTCTGA", "aa": "MQYDEKYPTANYYLFNADNPDWDHSQSRFVTDICYYLKEFLLPLAALIKLEISDEF", "aa_hexdigest": "ffe70e5c4958520bd30fd40fa2a1e424", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6759.490899999995, "isoelectric_point": 4.236289787292479 }, "id": "IGIGPGHMMC_1043", "locus": "GCA000148625_01017" }, { "type": "cds", "sequence": "contig_1", "start": 575876, "stop": 576154, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTGATGATGCTTGTAAAGAACTTCATCAAGCAACAAAAATTGCATCTGCTTTAGGAGCATTTGAAATAATATGCGATCCAACAAAAACAGTGATTTATCAGTTAGATTTATATAAAAGTTTAATTGCTTTTTTCAATAATATTTATACACTTAAACTTGATAAAACTAAACCGATTGAAGAACAATTAAAAACGAAGGAAATTAAAAATCTGCTAGGTAAAGCAATATATAAGGCAACATCATCATTGCAAAAAATGATGCCAGCTGGTTGA", "aa": "MVIDDACKELHQATKIASALGAFEIICDPTKTVIYQLDLYKSLIAFFNNIYTLKLDKTKPIEEQLKTKEIKNLLGKAIYKATSSLQKMMPAG", "aa_hexdigest": "71a7e2908dfc9a17f17d895a6dab4594", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10324.12279999999, "isoelectric_point": 8.86732006072998 }, "id": "IGIGPGHMMC_1044", "locus": "GCA000148625_01018" }, { "type": "cds", "sequence": "contig_1", "start": 576893, "stop": 576982, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAGCAGATTAAGATTAGGTTTAATAACAACATTAGCTAGTGGAACAATGGCTTTGCCATTAGTTTCATCTAGTTGTTGA", "aa": "MKKKSRLRLGLITTLASGTMALPLVSSSC", "aa_hexdigest": "bbf0ccf1d0fcb9f9d9dabe838d988e7e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3063.7445, "isoelectric_point": 11.105404472351072 }, "id": "IGIGPGHMMC_1045", "locus": "GCA000148625_01019" }, { "type": "cds", "sequence": "contig_1", "start": 578530, "stop": 578670, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAGAAGAAAATAATACAATGCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSEENNTMPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "4c9fa38b941faff729b4441fe9f4c8fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.020600000001, "isoelectric_point": 5.1057558059692365 }, "id": "IGIGPGHMMC_1046", "locus": "GCA000148625_01020" }, { "type": "cds", "sequence": "contig_1", "start": 578676, "stop": 578777, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 3.899520131836104e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "IGIGPGHMMC_1047", "locus": "GCA000148625_01021" }, { "type": "cds", "sequence": "contig_1", "start": 578820, "stop": 579266, "strand": "-", "frame": 3, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAATGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCTCCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQNDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "da2e25c48930bcd7456161a99c459ffc", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 578676, "stop": 579305, "observations": { "mutation": [ 578822 ], "stop": [ 578822 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 8.86e-94, "description": "Internal stop codon at 578822. Nonsense mutation around 578822.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "IGIGPGHMMC_1048", "locus": "GCA000148625_01022" }, { "type": "cds", "sequence": "contig_1", "start": 579301, "stop": 579519, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "IGIGPGHMMC_1049", "locus": "GCA000148625_01023" }, { "type": "cds", "sequence": "contig_1", "start": 579793, "stop": 580152, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKPYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFRKNGDTSCLTVL", "aa_hexdigest": "960afa9397d38dc95b18e550185890cb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14329.662599999978, "isoelectric_point": 9.686004066467284 }, "id": "IGIGPGHMMC_1050", "locus": "GCA000148625_01024" }, { "type": "cds", "sequence": "contig_1", "start": 580172, "stop": 580507, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_C4XEG8" ], "nt": "TTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGGTGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAATAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAA", "aa": "MFFVACYPKNAKVKNDKENLIDIRNNQVNSKGELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVIINKKGDKLIEA", "aa_hexdigest": "9db3cc00ba942d773f601ca6b1d027f1", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 580172, "stop": 580669, "observations": { "mutation": [ 580510 ], "stop": [ 580510 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEG8", "query_cov": 1.0, "subject_cov": 0.6727272727272727, "identity": 0.973, "score": 211.0, "evalue": 1.36e-68, "gene_start": 1, "gene_end": 111, "reference_start": 55, "reference_end": 165, "reference_sequence": "MNNYKKQGENMNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDEWLFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA" }, "paralog": false, "identity": 0.9757575757575757, "score": 319.0, "evalue": 2.29e-113, "description": "Internal stop codon at 580510. Nonsense mutation around 580510.", "psc": { "uniref90_id": "UniRef90_C4XEG8", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEG8", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12775.604599999984, "isoelectric_point": 8.7994348526001 }, "id": "IGIGPGHMMC_1051", "locus": "GCA000148625_01025" }, { "type": "cds", "sequence": "contig_1", "start": 580508, "stop": 580639, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGA", "aa": "MNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDE", "aa_hexdigest": "1bc3e1363e697e2abdb23ef609e7e097", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.7792, "isoelectric_point": 6.142328453063963 }, "id": "IGIGPGHMMC_1052", "locus": "GCA000148625_01026" }, { "type": "cds", "sequence": "contig_1", "start": 581183, "stop": 581854, "strand": "-", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "RefSeq:WP_013354726.1", "SO:0001217", "UniParc:UPI0001E32E9F", "UniRef:UniRef100_UPI0001E32E9F", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAACTCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTGCCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCATTGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTAA", "aa": "MRNSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISLSTNYVKKYSEINKLTKPNVDIFDN", "aa_hexdigest": "a5f8582448f56e1663c55de89df17269", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32E9F", "ncbi_nrp_id": "WP_013354726.1", "uniref100_id": "UniRef100_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E9F", "RefSeq:WP_013354726.1", "UniRef:UniRef100_UPI0001E32E9F" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32E9F", "uniref90_id": "UniRef90_UPI0001E32E9F" }, "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" }, "genes": [], "id": "IGIGPGHMMC_1053", "locus": "GCA000148625_01027" }, { "type": "cds", "sequence": "contig_1", "start": 582104, "stop": 582544, "strand": "-", "frame": 1, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTCTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLQPITSNNIVDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRLKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "73cecf916f7ee6a925d462771fe23be6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 1.0, "score": 280.0, "evalue": 2.05e-95, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "IGIGPGHMMC_1054", "locus": "GCA000148625_01028" }, { "type": "cds", "sequence": "contig_1", "start": 582563, "stop": 582685, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTAATGCCCGATAAACTAAAAACACTATAA", "aa": "MLGFKAAQIDSALTKVELNQDVESMIEEAIKLMPDKLKTL", "aa_hexdigest": "a0736f39cdc1f946d0b884009afc2447", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 34, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 1.8161655806586317e-08, "score": 30.797409057617188, "start": 2, "stop": 33 } ], "seq_stats": { "molecular_weight": 4463.2387, "isoelectric_point": 4.715101432800293 }, "id": "IGIGPGHMMC_1055", "locus": "GCA000148625_01029" }, { "type": "cds", "sequence": "contig_1", "start": 582767, "stop": 582943, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14520.12" ], "nt": "ATGGACTTAATTAATTTAAATGGCATTGGTCCTAGAGTAGCATTTAATGTTTTAAATGTTGGGTTTGATAAAGCTATAAATTTAATTAGAGATGGCAACATTGAAGAGATTGCAAAAATAGAATATATTAATCCAAGACTTGCAAGAATAATTGTTGAAGAATTACAAGACAAATGA", "aa": "MDLINLNGIGPRVAFNVLNVGFDKAINLIRDGNIEEIAKIEYINPRLARIIVEELQDK", "aa_hexdigest": "7ae3c0a1c22b3ec09c96811f4282438c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14520.12", "name": "HHH_5", "length": 56, "aa_cov": 0.9655172413793104, "hmm_cov": 0.9821428571428571, "evalue": 6.501507695261817e-12, "score": 41.834590911865234, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 6562.551599999997, "isoelectric_point": 4.853277015686035 }, "id": "IGIGPGHMMC_1056", "locus": "GCA000148625_01030" }, { "type": "cds", "sequence": "contig_1", "start": 582986, "stop": 583150, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01330.27" ], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTATGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAAATAAAAATGTATTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESDNVGYYVMFPDNKRIKLNQEIKMYLYEIKNDFT", "aa_hexdigest": "48687ab3903c0e2e0fa5a323cbe8c492", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01330.27", "name": "RuvA_N", "length": 51, "aa_cov": 0.9074074074074074, "hmm_cov": 1.0, "evalue": 9.942276251748226e-07, "score": 24.84882926940918, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 6587.6402, "isoelectric_point": 9.222540855407715 }, "id": "IGIGPGHMMC_1057", "locus": "GCA000148625_01031" }, { "type": "cds", "sequence": "contig_1", "start": 583163, "stop": 583633, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354729.1", "SO:0001217", "UniParc:UPI0001E32EA2", "UniRef:UniRef100_UPI0001E32EA2", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAATATTAACAAATATTTAGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAAGTTTTATTTGATTATAAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTGAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTTCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESISKLIDSKTIINFDIAKLQNINKYLGFGISDINLLKINQVLFDYKLLKTLRLENNLLELNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEEKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "0b50bfc9c9e5d4565aefdf77621919b4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32EA2", "ncbi_nrp_id": "WP_013354729.1", "uniref100_id": "UniRef100_UPI0001E32EA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EA2", "RefSeq:WP_013354729.1", "UniRef:UniRef100_UPI0001E32EA2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32EA2", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18860.733899999996, "isoelectric_point": 9.056083488464353 }, "id": "IGIGPGHMMC_1058", "locus": "GCA000148625_01032" }, { "type": "cds", "sequence": "contig_1", "start": 584227, "stop": 584418, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTCACCAAGAATTTTCAGAATTTTCTTCTGAAGAAATAAAAAACAAATTAAAAATTTATATAAGACGAGTTAACAAAACAATGTCATTATTAGAGTCAGATGTCTCATTATATTTATTTGTGAAAAGAATTATGATGAGATTTAAAGCAATTGAGTTATATGTTAAGGCTTATAAATATGATGAATGA", "aa": "MTHQEFSEFSSEEIKNKLKIYIRRVNKTMSLLESDVSLYLFVKRIMMRFKAIELYVKAYKYDE", "aa_hexdigest": "477b70a7ec42e919ee7ea532d57450a2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7733.013199999999, "isoelectric_point": 9.259352302551267 }, "id": "IGIGPGHMMC_1059", "locus": "GCA000148625_01033" }, { "type": "cds", "sequence": "contig_1", "start": 585454, "stop": 585954, "strand": "-", "frame": 2, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACACAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATTAATAAGCCTTCTAATTTTGTTTCACTAAGCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGCGCTAAATGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACTAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETQLKRALTNDATSKLIKEIINKPSNFVSLSNPFDVKTKIKQSFLKHQERIYEKFIQYCAKCLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "5b52e27ba7fdd5661ef452fc92677ef4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 585151, "stop": 585954, "observations": { "mutation": [ 585207, 585333, 585456 ], "stop": [ 585207, 585333, 585456 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9279999999999999, "score": 308.0, "evalue": 3.42e-104, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9288389513108615, "score": 496.0, "evalue": 1.54e-180, "description": "Internal stop codon at 585207, 585333, 585456. Nonsense mutation around 585207, 585333, 585456.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "IGIGPGHMMC_1060", "locus": "GCA000148625_01034" }, { "type": "cds", "sequence": "contig_1", "start": 586793, "stop": 587065, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTAATAATAAAGTTTTAATTAACAATAAGTTTTTAAATTTATCAAAAAATCAAGGTTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTNNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "2ad996294a11539a5db3f8419765547e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10697.456499999998, "isoelectric_point": 9.855878257751463 }, "id": "IGIGPGHMMC_1061", "locus": "GCA000148625_01035" }, { "type": "cds", "sequence": "contig_1", "start": 587177, "stop": 587401, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATCAATTAGTATCAACGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDQLVSTNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "a6934ca157d9ede901ff9207661877fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8673.306299999993, "isoelectric_point": 10.220898246765138 }, "id": "IGIGPGHMMC_1062", "locus": "GCA000148625_01036" }, { "type": "cds", "sequence": "contig_1", "start": 587465, "stop": 587671, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTTTTTCAAAACTATGGATTAAATTATGACAAATTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTATAAAATTCCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANYKIPETKTESLFKKISSKSLFEKM", "aa_hexdigest": "a9fa74e5e4f7a5d33cacb2c6b702f010", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 587362, "stop": 587671, "observations": { "insertion": [ 587392 ], "mutation": [ 587467 ], "stop": [ 587467 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.971, "score": 125.0, "evalue": 4.27e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9509803921568627, "score": 184.0, "evalue": 5.8e-62, "description": "Internal stop codon at 587467. Frameshift due to insertion around 587392. Nonsense mutation around 587467.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8026.149699999999, "isoelectric_point": 9.363984489440917 }, "id": "IGIGPGHMMC_1063", "locus": "GCA000148625_01037" }, { "type": "cds", "sequence": "contig_1", "start": 587909, "stop": 588268, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6", "UniRef:UniRef90_C4XDT6" ], "nt": "ATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGA", "aa": "MKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFT", "aa_hexdigest": "158bb4985583269e648457ca26a8d0c8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LIM6", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.6809999999999999, "score": 147.0, "evalue": 1.98e-43, "valid": false, "uniref50_id": "UniRef50_C4XDT6" }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 587846, "stop": 588325, "observations": { "mutation": [ 588266 ], "stop": [ 588266 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT6", "query_cov": 1.0, "subject_cov": 0.7484276729559748, "identity": 1.0, "score": 216.0, "evalue": 1.62e-70, "gene_start": 1, "gene_end": 119, "reference_start": 22, "reference_end": 140, "reference_sequence": "MLNNLIIKYNLNIIFLRRELVMKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFTWVLATIFLICTIVYAVRSK" }, "paralog": false, "identity": 0.9937106918238994, "score": 298.0, "evalue": 5.67e-105, "description": "Internal stop codon at 588266. Nonsense mutation around 588266.", "psc": { "uniref90_id": "UniRef90_C4XDT6", "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT6", "UniRef:UniRef50_C4XDT6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12818.22569999998, "isoelectric_point": 9.20713291168213 }, "id": "IGIGPGHMMC_1064", "locus": "GCA000148625_01038" }, { "type": "cds", "sequence": "contig_1", "start": 588435, "stop": 588734, "strand": "+", "frame": 3, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGA", "aa": "MINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVE", "aa_hexdigest": "7b2f40e187a81d911ad9a1851c7c4198", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "3-prime", "pseudogene": { "start": 588357, "stop": 588995, "observations": { "mutation": [ 588732 ], "stop": [ 588732 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4669811320754717, "identity": 1.0, "score": 200.0, "evalue": 9.8e-64, "gene_start": 1, "gene_end": 99, "reference_start": 27, "reference_end": 125, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9905660377358491, "score": 414.0, "evalue": 2.81e-150, "description": "Internal stop codon at 588732. Nonsense mutation around 588732.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "IGIGPGHMMC_1065", "locus": "GCA000148625_01039" }, { "type": "cds", "sequence": "contig_1", "start": 588738, "stop": 588995, "strand": "+", "frame": 3, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAA", "aa": "MNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK", "aa_hexdigest": "9c49cf9b22f296322c4146d5fc2768cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 588438, "stop": 588995, "observations": { "mutation": [ 588732 ], "stop": [ 588732 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4009433962264151, "identity": 1.0, "score": 167.0, "evalue": 4.48e-51, "gene_start": 1, "gene_end": 85, "reference_start": 128, "reference_end": 212, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9945945945945946, "score": 365.0, "evalue": 1.76e-131, "description": "Internal stop codon at 588732. Nonsense mutation around 588732.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "IGIGPGHMMC_1066", "locus": "GCA000148625_01040" }, { "type": "cds", "sequence": "contig_1", "start": 588998, "stop": 589300, "strand": "+", "frame": 2, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "RefSeq:WP_013354736.1", "SO:0001217", "UniParc:UPI0001E32EA9", "UniRef:UniRef100_C4XDT8", "UniRef:UniRef50_C4XDT8", "UniRef:UniRef90_C4XDT8" ], "nt": "ATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAA", "aa": "MNLVTTNCGLNLSYSITENVFSQLIDQCFEEVKNVKLANEARISFDKEQTNVAFVIDIKIKKGSGLQQVIENLTQELESRCLGLLDIKPRSIRLCFVGYY", "aa_hexdigest": "d78de6b5c1f9791c3efd907a1110f8c4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EA9", "ncbi_nrp_id": "WP_013354736.1", "uniref100_id": "UniRef100_C4XDT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EA9", "RefSeq:WP_013354736.1", "UniRef:UniRef100_C4XDT8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDT8", "uniref90_id": "UniRef90_C4XDT8" }, "psc": { "uniref90_id": "UniRef90_C4XDT8", "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_C4XDT8" }, "pscc": { "uniref50_id": "UniRef50_C4XDT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT8" ] }, "genes": [], "id": "IGIGPGHMMC_1067", "locus": "GCA000148625_01041" }, { "type": "cds", "sequence": "contig_1", "start": 589405, "stop": 589899, "strand": "-", "frame": 2, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_C4XDT9", "UniRef:UniRef90_C4XDT9" ], "nt": "ATGAAAATAAGTAGCGATGCTTCATTAATTGATACTCAATTGGCTAAAAGAGCAGGAACAATTCTTAAGTTAGTTGAATCAGTAAGTTCACACATGAAATTCGAAAAAGATGTTATGACACAAGTTGCAGCATACCGTTCAGCAGTTGCTACAATTCGAGAACAATCAGGAAATACTTCAGCAGATGCTATGGCAGTACGTCAAAACTTTGAAAGTCAAACAGAATCATTCTTAACAAGATTTATGGCTCAATTCGAAAAATATCCAGAACTTAAGAGTGCTGAATTAGTTAAAGGTTTAAATGATGAAATCGTTGAATGTGAAAACAACATTGCTTCAGCTAGAAGAATTTACAATACTTCAGCCACAACATTTAACCAAGAAATCTTTACATATCCAAAAAGTGTTGTTGCTTCAAGTATGAGATTAACAACAATTCTATTATTCCAAGCTAAAGAAGCTGATAGACAAGATGTTAGTGTGAAATTGTATTAA", "aa": "MKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY", "aa_hexdigest": "e8ec4ee496bed29dc3dfc5e235ceb26e", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "lemA" ], "truncated": "3-prime", "pseudogene": { "start": 589405, "stop": 590130, "observations": { "mutation": [ 589926, 589974 ], "stop": [ 589926, 589974 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT9", "query_cov": 1.0, "subject_cov": 0.6804979253112033, "identity": 1.0, "score": 301.0, "evalue": 3.52e-102, "gene_start": 1, "gene_end": 164, "reference_start": 78, "reference_end": 241, "reference_sequence": "MCIEHKNNYIEGEKMANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFIWYISFIFLIPIFIHISWVNYFRRQQMKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY" }, "paralog": false, "identity": 0.9875518672199171, "score": 459.0, "evalue": 7.4e-167, "description": "Internal stop codon at 589926, 589974. Nonsense mutation around 589926, 589974.", "psc": { "uniref90_id": "UniRef90_C4XDT9", "product": "LemA family protein", "uniref50_id": "UniRef50_C4XDT9", "kegg_orthology_id": "K03744", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT9", "UniRef:UniRef50_C4XDT9", "KEGG:K03744" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT9" ], "product": "LemA family protein" } }, "id": "IGIGPGHMMC_1068", "locus": "GCA000148625_01042" }, { "type": "cds", "sequence": "contig_1", "start": 589972, "stop": 590088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAATTTATACAATCGTGAAAACAGTCGTGCTATTAATGAAAAAGGCTATGAACCACGTTCAATGAGTACAGAAGTACCAGTTAAAGTAAACTTTTTTGGAAAATTTATTTGA", "aa": "MANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFI", "aa_hexdigest": "3130e5d19c8ce1b5d638747949276b32", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4438.0085, "isoelectric_point": 9.39608974456787 }, "id": "IGIGPGHMMC_1069", "locus": "GCA000148625_01043" }, { "type": "cds", "sequence": "contig_1", "start": 590174, "stop": 590371, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE", "PFAM:PF02911.23" ], "nt": "ATGCAAGAAGCAATCAATATTATTAGAGCTTATGAGATGAATCCTGGTGCTTACACTTTTATTGATAATAAAAGAATTAAGATCTTTTTTGCAACCAAAAAAGAAATTAAAAATGTACCTAAATTAAAATTTAAAGATGGTTATTTATTTGCCACTGATTATCAATATGAATCTAAAAAACGAGTTAAGCTGGCATAG", "aa": "MQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA", "aa_hexdigest": "161e228b91d84bd13897f2a070d15771", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF014FE", "query_cov": 0.9846153846153847, "subject_cov": 0.810126582278481, "identity": 0.531, "score": 76.6, "evalue": 4.47e-17, "valid": false, "uniref50_id": "UniRef50_UPI002FF014FE" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF014FE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 33, "aa_cov": 0.5076923076923077, "hmm_cov": 1.0, "evalue": 5.943395344023241e-07, "score": 25.66942024230957, "start": 3, "stop": 35 } ], "seq_stats": { "molecular_weight": 7740.009099999999, "isoelectric_point": 9.776259803771971 }, "id": "IGIGPGHMMC_1070", "locus": "GCA000148625_01044" }, { "type": "cds", "sequence": "contig_1", "start": 590483, "stop": 591010, "strand": "-", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_D3VRI0", "UniRef:UniRef90_C4XDU0" ], "nt": "ATGAAATTATTATTAGCCGGAACTCCTGCTTTTGCAGTTGAAATATTTGAAAAATTAATCAACAACTTTGAAGTTGTTGCAATTGTTTCGCAACCTGATCGCCCAAGTGTCAGAGGTCATAAATTAGCTCCAACGCCAACAAAATTATTAGCTCAAAAATATAACATTAAATGTTTTCAACCAGAAAAAATATCACAAATAAAAGATGAATTACAAACTTTAAATTATGACTATTTAATTACCTGTGCCTTTGGTCAATATATTCCTGAAAGTGTTTTAAATATTGCTAAAAAATTAAGTCTAAATATTCATGGTTCTTTACTTCCAAAATATCGTGGAGCAGCACCAATTCAATATTCACTTTTAAATAATGATCAAGAAACTGGTATTAGTCTAATGGAAATGATTAAACAAATGGATGCAGGTGATGTTTTTGTCCAAAAAGCCATTAAAATAGATGAATATGACACAGCAAGTACTTTATTTAATAAGCTTTCAAAGCTTAGTGCAGATAACATTGTGCAATGA", "aa": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQ", "aa_hexdigest": "82b0558bc26312d350f2db7090d9a928", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF2EE64", "query_cov": 0.9942857142857143, "subject_cov": 0.9560439560439561, "identity": 0.644, "score": 227.0, "evalue": 1.77e-73, "valid": false, "product": "methionyl-tRNA formyltransferase", "uniref50_id": "UniRef50_A0A3B0PK42" }, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 590180, "stop": 591010, "observations": { "mutation": [ 590485 ], "stop": [ 590485 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU0", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 1.0, "score": 342.0, "evalue": 1.43e-117, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQWLNDLDQNKLKRIKQDESKVTLSPKLEKEKALLNESLTMQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA" }, "paralog": false, "identity": 0.9963768115942029, "score": 537.0, "evalue": 7.71e-197, "description": "Internal stop codon at 590485. Nonsense mutation around 590485.", "psc": { "uniref90_id": "UniRef90_C4XDU0", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_D3VRI0", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU0", "EC:2.1.2.9", "UniRef:UniRef50_D3VRI0", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRI0" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "IGIGPGHMMC_1071", "locus": "GCA000148625_01045" }, { "type": "cds", "sequence": "contig_1", "start": 591252, "stop": 591473, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAGGTATATTTGAACATGATAGCAATAATGTAAAAATTATTTATGTTGTTTCATTATTAGCACATATAATCATTCATATTACAATGATTGCTATTCTTGTTTATGCAATTAAAGCATTTGTAAAATCAATAGCTTCGAAAAATGTAATTAGCATTATTTTAAATATTTTGGTATTTGCGGCAGTTGTTTTAACAATTATTTGTTATGTTGGCTGA", "aa": "MIKGIFEHDSNNVKIIYVVSLLAHIIIHITMIAILVYAIKAFVKSIASKNVISIILNILVFAAVVLTIICYVG", "aa_hexdigest": "0a424a6c8e1bc6c805bd31726ec706d8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8023.816999999997, "isoelectric_point": 9.30312633514404 }, "id": "IGIGPGHMMC_1072", "locus": "GCA000148625_01046" }, { "type": "cds", "sequence": "contig_1", "start": 591949, "stop": 592092, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTCAATAGCGGCGTATATTTTAATAAGATTATTTGATAAAAAACGTTTAGCTGACATTAGTGATTTTCTCGGTGAACGTTCTGTTGAAATTCATGAACAAGTTGAGCAAAGCAAAAAAGATCGTAACAAAATGTGA", "aa": "MASSIAAYILIRLFDKKRLADISDFLGERSVEIHEQVEQSKKDRNKM", "aa_hexdigest": "49879b9c837c8368de215a3a0fab766b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5481.2681, "isoelectric_point": 8.120969581604005 }, "id": "IGIGPGHMMC_1073", "locus": "GCA000148625_01047" }, { "type": "cds", "sequence": "contig_1", "start": 592384, "stop": 592731, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATATCAACCTATTCAAAATTCAGCAAGTATCGAAATTGTTCAAAATAACCACGACTACAAATTCAATAGTGTAAGTAGTCGTGTTTATCGTGATCCAAAAAATCAAATATCACCTCGTGTTTATCGCGTCATCCGTGCTGAACAAATGCTTAAGATTTTAATCCTTTCTATTTCATTTGTAATTATGGCTGCTTCAATTATTCTCTTCACATTAGTCTATGCTAAAGTTGGTATCTTCAACAAAGAACAAAGATTAGTTGGCTACCTTGTACTTTTTGGATCAAGTGCCTTTATCTCAATGGCAATTGGTTTTAAAAATCTGATTGAAAATATTCAATGA", "aa": "MRKYQPIQNSASIEIVQNNHDYKFNSVSSRVYRDPKNQISPRVYRVIRAEQMLKILILSISFVIMAASIILFTLVYAKVGIFNKEQRLVGYLVLFGSSAFISMAIGFKNLIENIQ", "aa_hexdigest": "1f470b6d0be182ce1045367f9bd195de", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.433999999987, "isoelectric_point": 10.056503868103025 }, "id": "IGIGPGHMMC_1074", "locus": "GCA000148625_01048" }, { "type": "cds", "sequence": "contig_1", "start": 592959, "stop": 595754, "strand": "-", "frame": 3, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "GO:0005524", "GO:0007059", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_Q98PK8", "UniRef:UniRef90_C4XDU3" ], "nt": "ATGGATGATGTTATCTTCGCTGGTTCTAAAACTGCAAAACCTATGGATAAAGCAGTAGTTACTTTAACTTTTGATAATCGAAATGGTATTAGTTCAATCCCGCATGAAACTATTTCAATTAGTCGTGTGCTTGAAAGAGGAAATGGAAACAACCAATATTTCTTAAATGGCGAACCATGTCGTCAAAAAGATATTAAAGATATTGCTATGGAAAGCGGTATTGGTAAAAGTTCATTAGCTATTATTTCTCAAGGTACAGTTAGTGATATTGCTGAAGCTACACCTGAAGAAAGAAAGTCTATTTTTGAAGAAGCTGCTGGTGTTTCAAAATACAAATTTCGTAAAAAAGAAGCTTTAAGCAAATTGGCTAAAACTCAAGAAGGTTTAGACCAAATTAAACTTGTTATTAGTGAAATTGAAAGAAAATTAATTCCATTAAGAAAACAAGCAGAAAAAGCTAAAGTTTACATTGCTAAAAGTGAAGAATTAAAAGCTGTTGAAGTTGGTCTTTTAGTAGACAACATCAAAACTTTTGGTGATATGTACGAAAGACTTGTAACTGAACTTGAAGGTGTTCAAGAAACTAAAAATGATCTTGATGCTCGTATTTCATCAGCTGAAGCTGCTATTACTCAAAATACTAAATTCAGATCAGAAGTTGAAGCCGAATTAAAAGAACTTTCAATTGAATATGAAACAGTTAAAGAAAGATTAAACAAATTAAACATTTCTTTAGCTAAAGAAAATGAACGTGAAAGATTAATTGCTGCTGGTGAAATTCAAGCAAACCAAGCTGAACAAATCGAAGCATACAAAACTATGCTTACAAGACATGCAAATAAATTAAATTACTACGCTCGTGAAATTGAAAATCAAGCAAACCAAATTGCTGAAAACCAAAAAACAATTTCAAAATATGACGAAGAAATTAATTTAATGAACATTAAAATTAACAAAAAACGTACAGACTTATTAAAAATTGAAACACAGTTAAACTTGCTTAACCAACAAAAAGAAAGTAACTCTTTATTATTTAAAGGGACCAAAAACATTTTAGAAAATAAAGCTTACTTTGGAAAAGCACTAAAAGGAACTGTAGCTGATTTAATTAAAGTTACAACAGAATATATTCCTGCTATTGACTCAATTTTAGCTAATGCTTCACAACACTTAGTGGTTGATAAAGCTGAAACAGCAGTTAAAGCTGTTAACTTCTTAAAAGATAATAATGGTGGTCGTGCTACATTTATTCCACTCACATCAATTCAACCAAAAATGATTCGTGATGATCATCTTTTAGCAATCCAAGGACACCCTGGATTTATTGATGTTGCAAGTAATTTAGTAACAATTGATCCTCAATATGAAATTCTTAATAAATTCCTTTTAGGTAATGTTATTGTTGCTAGCGACATTGAAAATGCTAACAAAATTTCAAGTATTCTCGAACGTCGTTACATGGTAGTTTCACTTGATGGTGACACCATTCGTTCAGGCGGTGTGATTATTGGTGGAGCTAAAAATACAGCTCAATCATTACTTGGATTTGATGAAAAAATTAAAGAGCTTGAAAATATTGTTCCAGGTATGAGAGCAGCAGTTCAAGCTGATGAATCAAAAATCAATGAAATAAGAAACTATCGTCGTAGTGCTCTTGAATTAGACAGCCAATTAAAACAACAAATTACTAGTCTAAAAACCCAAAAGAGCAGTGAACAAGGTCTTTACGACGAATTACAATTAAAACTTCAAAATATTACAAACAAAGAATTCAAAGTTAAAGAAGGCGTTTCAGCATCAAGCCCTGAAGCTTCAATTGAAGTTTTAGAAAGTCAAAAAATTGCTCTTGAATCACAATTCAGAGCTAAAAATGAACGTGTAAATACAATTGCTGCCGAAATCAATTCAACTTTAGCTCTTAAAAATGATTATGAAGCTACACGTCGTAAATTAACAGATGCTTTCTCAGCTAAAATTGGTGAAAGAGAAAGATCAAAAACTTTACTTGAACAAAGTAGAGAAAGACTTGCTTCTCAATACAAAATGACATTTGAAGCAGCTGAAAAAGTTCATCACTTAGAAATGAATCGTGATCAAGCTGAAGAAATTGTTCGTCAATTAAGAGAAGAAATTGAAGGCTTAGGTAACGTTAACTTAGAAGCTCTTGAACAATTAGTTGAAGAAGAAAAACGTTACAATGAATATGCCGAAGGTGAAAGAGAATTAAGTGCTGCAAAAGAAACACTTGAAAGCGCTATTGCAGACATGGATAAAATCATTATCACTCGTCTAACTAACATTATTAATGATGTTAATGATGAATTTAACAATGTCTTTAGAACAATGTTCGGTGGTGGTACTGCAAAATTATTTTTCAGCAACCCTAAAGATATTTTAGAATCAGGTGTTGAAATCGAAGCTCAACCTCCTGGAAAAAATATTAAAAACCTTAAATTATTCTCAGGCGGTGAAAAATCACTTATCGCTATCTCATTATTATTTGGTATTCTTAAAGCTCGTCCATTACCTCTTTGTATCCTTGACGAAGTTGAAGCTGCACTTGACGAAGCTAATGTTGTTCGTTATGCAGAATACCTTCAACAACTTAAAGAAAAAACTCAATTCTTGGTTATTACACACCGTCATGGAACAATGTCAAGAGTTGACACATTATTTGGTGCAACAATGCAAAACCGTGGTGTTACAAGCTTCTTCAGCATTCAATTAGCAGATGCTAAAAAACTTGTTGCAGACGAACAAAAAGAAGAATTAGAACCTTCAAACAAAAACAAATAA", "aa": "MDDVIFAGSKTAKPMDKAVVTLTFDNRNGISSIPHETISISRVLERGNGNNQYFLNGEPCRQKDIKDIAMESGIGKSSLAIISQGTVSDIAEATPEERKSIFEEAAGVSKYKFRKKEALSKLAKTQEGLDQIKLVISEIERKLIPLRKQAEKAKVYIAKSEELKAVEVGLLVDNIKTFGDMYERLVTELEGVQETKNDLDARISSAEAAITQNTKFRSEVEAELKELSIEYETVKERLNKLNISLAKENERERLIAAGEIQANQAEQIEAYKTMLTRHANKLNYYAREIENQANQIAENQKTISKYDEEINLMNIKINKKRTDLLKIETQLNLLNQQKESNSLLFKGTKNILENKAYFGKALKGTVADLIKVTTEYIPAIDSILANASQHLVVDKAETAVKAVNFLKDNNGGRATFIPLTSIQPKMIRDDHLLAIQGHPGFIDVASNLVTIDPQYEILNKFLLGNVIVASDIENANKISSILERRYMVVSLDGDTIRSGGVIIGGAKNTAQSLLGFDEKIKELENIVPGMRAAVQADESKINEIRNYRRSALELDSQLKQQITSLKTQKSSEQGLYDELQLKLQNITNKEFKVKEGVSASSPEASIEVLESQKIALESQFRAKNERVNTIAAEINSTLALKNDYEATRRKLTDAFSAKIGERERSKTLLEQSRERLASQYKMTFEAAEKVHHLEMNRDQAEEIVRQLREEIEGLGNVNLEALEQLVEEEKRYNEYAEGERELSAAKETLESAIADMDKIIITRLTNIINDVNDEFNNVFRTMFGGGTAKLFFSNPKDILESGVEIEAQPPGKNIKNLKLFSGGEKSLIAISLLFGILKARPLPLCILDEVEAALDEANVVRYAEYLQQLKEKTQFLVITHRHGTMSRVDTLFGATMQNRGVTSFFSIQLADAKKLVADEQKEELEPSNKNK", "aa_hexdigest": "3233d6bd1b66605abbdcf2f33b755637", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU3", "query_cov": 1.0, "subject_cov": 0.9300699300699301, "identity": 0.9990000000000001, "score": 1699.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_Q98PK8", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PK8" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "IGIGPGHMMC_1075", "locus": "GCA000148625_01049" }, { "type": "cds", "sequence": "contig_1", "start": 595797, "stop": 595934, "strand": "-", "frame": 3, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687", "UniRef:UniRef90_UPI0024CCD326" ], "nt": "ATGAAACTCATTAAAGTTGAAGCCCACGGATTCAAATCATTTGCTGATCCTATTGTGCTTAAATTTGATGGTGGAGTAGCCGGAATTGTTGGCCCTAATGGTTCTGGTAAATCAAATATTAACGATGCTATCAAATGA", "aa": "MKLIKVEAHGFKSFADPIVLKFDGGVAGIVGPNGSGKSNINDAIK", "aa_hexdigest": "566a740d3cb0dc930db18b8fd124f59e", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 595701, "stop": 595934, "observations": { "mutation": [ 595799 ], "stop": [ 595799 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024CCD326", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.9109999999999999, "score": 87.8, "evalue": 7.69e-22, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MKLVKLEAHGFKSFADPIVLRFDGGVAGIVGPNGSGKSNINDAIRWVLGEQSSKELRGDTMEDVIFAGSKTVQPMNKNRFL" }, "paralog": false, "identity": 0.8441558441558441, "score": 136.0, "evalue": 1.61e-43, "description": "Internal stop codon at 595799. Nonsense mutation around 595799.", "psc": { "uniref90_id": "UniRef90_UPI0024CCD326", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024CCD326", "UniRef:UniRef50_UPI0021DFD687" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687" ], "product": "AAA family ATPase" } }, "id": "IGIGPGHMMC_1076", "locus": "GCA000148625_01050" }, { "type": "cds", "sequence": "contig_1", "start": 596032, "stop": 596607, "strand": "-", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_C4XDU4", "UniRef:UniRef90_C4XDU4" ], "nt": "GTGAGTGAAAGAGTTGGCAATTATCAAGATCTTGAATTTCTCGGAGACGCTATTTTACAGTATCTTTCGTCAGATTTTGTATTCAAAAAATTTCCAAATTTAAGTGCTGGTAATATGACTTTAATTCGTTCTAAATTAGTTTGCACAAAGTCACTAAATGAAATTAGCACTAAAATTGGATTAAAAGATTTTTTACTAACTGCACCTGGTCAAATGAGTAAAGATGCTAAACGCTCAGTTAAAGTTGGCGCTGACATTTTTGAAGCCTTTATTGCTGCTATTTATTTGGATACAAATCTAAAAGTAGTCAAAGAATTTTTAAACAAAACTGTTTTTGATATCAAAATTGGAGACTTTGACATCAAGCATCTTAAAGATGCTAAAACTGCTTTTCAAGAACAAATTCAATCATTCAGTAAACTAGCTGTTATGTATATAGTTAGTGAAAAAAGTCCAACTGAATTTGAAGCTCACGCAGTTCACGACAATAAAATTTATGGTATTGGTTTTGGTAAAAGCAAAGCTGAAGCCGAAGAAAATGCTGCACTAGATGCATTGCATAAAATGAAAAAATAA", "aa": "MSERVGNYQDLEFLGDAILQYLSSDFVFKKFPNLSAGNMTLIRSKLVCTKSLNEISTKIGLKDFLLTAPGQMSKDAKRSVKVGADIFEAFIAAIYLDTNLKVVKEFLNKTVFDIKIGDFDIKHLKDAKTAFQEQIQSFSKLAVMYIVSEKSPTEFEAHAVHDNKIYGIGFGKSKAEAEENAALDALHKMKK", "aa_hexdigest": "8e9a13a2f6ad83f7d183e08768bbcbaa", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU4", "query_cov": 1.0, "subject_cov": 0.809322033898305, "identity": 0.995, "score": 371.0, "evalue": 2.38e-129, "valid": true, "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_C4XDU4", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU4" ], "product": "Ribonuclease 3" }, "genes": [ "rnc" ], "id": "IGIGPGHMMC_1077", "locus": "GCA000148625_01051" }, { "type": "cds", "sequence": "contig_1", "start": 597295, "stop": 597756, "strand": "-", "frame": 2, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "EC:2.3.1.274", "GO:0008610", "GO:0043811", "KEGG:K03621", "SO:0001217", "UniRef:UniRef50_Q98R48", "UniRef:UniRef90_C4XDU5" ], "nt": "ATGTACAAAATAGCATTTGATATTAATGGCAATGACAACGGCATTAATTCAGCTTTAGCAGCAACTAAATTATTTTTAAAAACCAACAAAAATTATGCAATTATTTTAGTTGGTCCTAAGGCTGAAATTGAAAAATATTATGGCAAAAACATGCCAGAAAATTTAGAAATAATTGACAACTCATCAGTGCCAAGCGATGTTAAAAACATTCGCCAATCTTTAAGAGAAAATACCTCAATGAATGTTGCACTTGATTTAGTAGCAAATAAAAAAGCAGATGCTGTACTTTCTTCAGGAGACTCTGGCACATATCTTGCAGCTGCTACTTTTAAAGTTAAAAGATTAGAAGGCATTTCAAGAGCAGCATTTATGCCCTTAATGCCTACAATCACAGGACGTAAATTCTTACTTCTTGATGTTGGAGCTAACTTAGAAACCACAAGTGATTATTTAGTTGAATGA", "aa": "MYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVE", "aa_hexdigest": "3c509b4348dba4600e103eb9c6b8ce5d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "plsX" ], "truncated": "5-prime", "pseudogene": { "start": 596992, "stop": 597813, "observations": { "mutation": [ 597297 ], "stop": [ 597297 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU5", "query_cov": 1.0, "subject_cov": 0.43714285714285717, "identity": 1.0, "score": 292.0, "evalue": 5.22e-97, "gene_start": 1, "gene_end": 153, "reference_start": 20, "reference_end": 172, "reference_sequence": "MVLNLTTTSLLVLNKIRSKMYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVEWTKIANSFDRVLLKNLAPRCALINIGTEDYKGTELIKEANNKLKEQHQLNYIGFQEPRDLLNGVCDVAVIDGYGGNLILKSLEGAILSFKNLLTSKIKLKPIRKLGYLLARGAFKDVAETLDYRNVGAAWIAGVNGVVIKSHGSSDEKAYYGALNQIKLALDNHILSEVKKDLNINEE" }, "paralog": false, "identity": 0.9926739926739927, "score": 522.0, "evalue": 5.61e-190, "description": "Internal stop codon at 597297. Nonsense mutation around 597297.", "psc": { "uniref90_id": "UniRef90_C4XDU5", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q98R48", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU5", "EC:2.3.1.274", "UniRef:UniRef50_Q98R48", "KEGG:K03621", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R48" ], "product": "Phosphate acyltransferase" } }, "id": "IGIGPGHMMC_1078", "locus": "GCA000148625_01052" }, { "type": "cds", "sequence": "contig_1", "start": 597770, "stop": 599395, "strand": "-", "frame": 1, "gene": "dhaL", "product": "DhaL domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354744.1", "SO:0001217", "UniParc:UPI0001A8C31B", "UniRef:UniRef100_C4XDU6", "UniRef:UniRef50_A0A449B6P5", "UniRef:UniRef90_C4XDU6" ], "nt": "ATGACTAAGATTTTAGACGGTAAGCTTTTTTGTCAACTTTTTATTTCCGGAGCTAATAACATTATTAACTCTAAAAATAAGATCGACGCTTTAAATGTTTTTCCTGTTCCAGATGGCGACACAGGAACTAACATGTCATCAACAGCTCAAGCTGCTGCTGATGCTTTGGCTTTAAATAACTCAAATCACTTAGGTGAAGTAACTAACACTATTTCAAGAAATATGCTTTTAGGAGCTCGCGGAAACTCAGGTGTTATTTTAAGCCAAATTTTTAAAGGTTTTGCAATTGCGTTTAAAGAAGTTGAAAGCGTTGACATTAAAGGACTTTTAGAAGGATTTAAGCAAGCGACTAAAAAAGCTTATTCATCAGTTTTAAAACCTGTTGAAGGTACCATTTTAACAGTTATTAGAGAAACAACAGAAGCATTAGAAAAAGAAGTAAATAATAAAACTTCACTAGAAGAATTTTTCAAATTAGCTAAAAATGCAGCTCGTAAAGCATGTGACAATACTCCTAACAAATTAAAGATCTTACGTGAAGTTGGCGTAACTGACTCAGGTGGTGAAGGATTATATACAATTATTAGTGGTATGGAAGCAGCATTAAATGCTGAAGCCATTAAAATTAGTGAATCAGAAGAAAACATTGATACTTTTATCAGCTCAAAAGAAGTTTATGATGGCGAATTTGGTTATTGTACTGAATTCATTATTGAATTAAATGACGAAAAAGACTTTGATAAAGCTAATTTTGAAAAATCATTACTTAAAAAAGCTAATTCTTTAGTTGTAGTTCAAGATGAAAAAATTGTTAAAGTTCATGGTCATACATTAAAACCTGGTGATATGTTAAACTTTGGTCAAAAATATGGTGAATTTATTAAAATAAAGTCAGAAAATATGACTCGTCAAGCAAGTGAAAGTCGTAATAAAAACGTTATTATTAACCAAAATGATGAACAATCAAACAAAAAATGTGCCATTATTTCATGTAACTTAGGCAGTGGAATTATTGCAAGAATGAAAGAATTTGGAGTTGACGCTATTATTGAAAGTGGTCAGACACAAAACCCTTCAGCTCAAGATATTATTGATGCAATTAACTCAGTAAATAGCAAAAACGTTTTCATTTTACCTAATAACTCAAACATTTTCCTAGCAGCTGAACAAGCAGCTCAAGCTATTACAAATAAAAAGGTATTTATTATTCAAACTAGAAGTCAAATTCAAGGATTAACAGCTTTAATGAACTTTAATTCTGAATCAAGTCCTAAAGAAAACCGTGAAACAATGTCTGAATCTATTAAGCTTGTAACCACAGCTGAAGTAACCCAAGCAATTAGAAGCACAAAAATTGACAATGTTAAAATCAAAGAAGGTCAATTCATTGGCATTACTGATGGCAAGATTTTAGTTAGTGAAAATAACTACATTGATGCAGCTAAAAAATTGATTAAAAAATCAATTAACAGTGATCATGAACTTGTTACAGTTTACTATGGAAATGATGCAAGTGAAGTTGATGCTAACGAAATTAAAAACTTTTTAGAAAGCCATTATGATGTTGAAGTTGAAGTAGTGAATGGTGCTCAACCTAACTACCACTTCATTATTGGTTTTGAATAA", "aa": "MTKILDGKLFCQLFISGANNIINSKNKIDALNVFPVPDGDTGTNMSSTAQAAADALALNNSNHLGEVTNTISRNMLLGARGNSGVILSQIFKGFAIAFKEVESVDIKGLLEGFKQATKKAYSSVLKPVEGTILTVIRETTEALEKEVNNKTSLEEFFKLAKNAARKACDNTPNKLKILREVGVTDSGGEGLYTIISGMEAALNAEAIKISESEENIDTFISSKEVYDGEFGYCTEFIIELNDEKDFDKANFEKSLLKKANSLVVVQDEKIVKVHGHTLKPGDMLNFGQKYGEFIKIKSENMTRQASESRNKNVIINQNDEQSNKKCAIISCNLGSGIIARMKEFGVDAIIESGQTQNPSAQDIIDAINSVNSKNVFILPNNSNIFLAAEQAAQAITNKKVFIIQTRSQIQGLTALMNFNSESSPKENRETMSESIKLVTTAEVTQAIRSTKIDNVKIKEGQFIGITDGKILVSENNYIDAAKKLIKKSINSDHELVTVYYGNDASEVDANEIKNFLESHYDVEVEVVNGAQPNYHFIIGFE", "aa_hexdigest": "daf6d8778c82e3384ca53ddab710e206", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C31B", "ncbi_nrp_id": "WP_013354744.1", "uniref100_id": "UniRef100_C4XDU6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31B", "RefSeq:WP_013354744.1", "UniRef:UniRef100_C4XDU6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU6", "uniref90_id": "UniRef90_C4XDU6" }, "psc": { "uniref90_id": "UniRef90_C4XDU6", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_A0A449B6P5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6P5" ], "product": "Dihydroxyacetone (Glycerone) kinase, DhaK2 subunit" }, "genes": [ "dhaL" ], "id": "IGIGPGHMMC_1079", "locus": "GCA000148625_01053" }, { "type": "cds", "sequence": "contig_1", "start": 599388, "stop": 599678, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354745.1", "SO:0001217", "UniParc:UPI0001A8C31C", "UniRef:UniRef100_C4XDU7", "UniRef:UniRef50_C4XDU7", "UniRef:UniRef90_C4XDU7" ], "nt": "ATGACTTTAGAAGAAATTAGAAAAGTTTTTTTGAATATTCTTGAATCAACGCCGGGGGTTTATGGAATCTGTGAAAATGCAGAACAATGTCCTAATTTAGGCCAAGAATATTTAAGTATTCAAAATATAAACAATGAATGGCATTTCAGCGCTACAATTATTATTTTAAAATATGCTAATGCAAAAAATGTTGTTAACTCAATTAGTTCTCACTTAAGATACGAACTCAATAAGAAAAAGGAACATTTAGGCAAATTAAATGTATTTATAGGAGGTCTTGCAAATGACTAA", "aa": "MTLEEIRKVFLNILESTPGVYGICENAEQCPNLGQEYLSIQNINNEWHFSATIIILKYANAKNVVNSISSHLRYELNKKKEHLGKLNVFIGGLAND", "aa_hexdigest": "3a51432363c8cd1ca400ef9de6aa183e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C31C", "ncbi_nrp_id": "WP_013354745.1", "uniref100_id": "UniRef100_C4XDU7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31C", "RefSeq:WP_013354745.1", "UniRef:UniRef100_C4XDU7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU7", "uniref90_id": "UniRef90_C4XDU7" }, "psc": { "uniref90_id": "UniRef90_C4XDU7", "uniref50_id": "UniRef50_C4XDU7" }, "pscc": { "uniref50_id": "UniRef50_C4XDU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10877.31659999999, "isoelectric_point": 6.275900077819824 }, "id": "IGIGPGHMMC_1080", "locus": "GCA000148625_01054" }, { "type": "cds", "sequence": "contig_1", "start": 599812, "stop": 600354, "strand": "+", "frame": 1, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_C4XDU8", "UniRef:UniRef90_C4XDU8" ], "nt": "ATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGA", "aa": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQR", "aa_hexdigest": "bfc34cb513bf9df5df8f4a04af468ad4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "recO" ], "truncated": "3-prime", "pseudogene": { "start": 599812, "stop": 600492, "observations": { "mutation": [ 600352 ], "stop": [ 600352 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU8", "query_cov": 1.0, "subject_cov": 0.7964601769911505, "identity": 1.0, "score": 362.0, "evalue": 3.42e-126, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQRWTKELKSIYYMYNDLKIFLQICISKVNQTIFYELNKYLEENGIYLN" }, "paralog": false, "identity": 0.995575221238938, "score": 455.0, "evalue": 3.71e-165, "description": "Internal stop codon at 600352. Nonsense mutation around 600352.", "psc": { "uniref90_id": "UniRef90_C4XDU8", "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_C4XDU8", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU8", "UniRef:UniRef50_C4XDU8", "KEGG:K03584", "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU8" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" } }, "id": "IGIGPGHMMC_1081", "locus": "GCA000148625_01055" }, { "type": "cds", "sequence": "contig_1", "start": 600675, "stop": 601277, "strand": "+", "frame": 3, "gene": null, "product": "Nitroreductase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9", "UniRef:UniRef90_C4XDU9" ], "nt": "ATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGA", "aa": "MSFIENNLKRGSTRDYQIGYEISEEDKEKLIKTIQNAPTSNNYFASSVIVIEDQNTKDKLAEILQQKQVRECSMFFIFLADNNRIDYVLKTKNKNIENKTLNSLLLSVGDAFIQATMLQDAATELDLGTCFIGGVRGHISEINRLLKIKNSAFPAVALTVGKAAKKADLKPKINRIYYEQYSYAQVVDEIVPYNEKLTNY", "aa_hexdigest": "ba2d98a50dfbad4f7a08c7e2fc3e9f55", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_C4XDU9", "query_cov": 1.0, "subject_cov": 0.8403361344537815, "identity": 1.0, "score": 386.0, "evalue": 4.21e-135, "valid": true, "product": "Nitroreductase domain-containing protein", "uniref50_id": "UniRef50_C4XDU9" }, "pscc": { "uniref50_id": "UniRef50_C4XDU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9" ], "product": "Nitroreductase domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1082", "locus": "GCA000148625_01056" }, { "type": "cds", "sequence": "contig_1", "start": 601460, "stop": 601603, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGA", "aa": "MAKFKRVFMIVTDGLGIGPDKDQRAFGDKGANTILSASKSSMFFIDT", "aa_hexdigest": "073303c3d294ddbd4feec3b74c7ef7c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5113.885799999999, "isoelectric_point": 9.397443580627442 }, "id": "IGIGPGHMMC_1083", "locus": "GCA000148625_01057" }, { "type": "cds", "sequence": "contig_1", "start": 601730, "stop": 601963, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGA", "aa": "MMGIKTMVPFPTFTENGFPQDLLDELSKAFDGRKIICNKSGSGTEMIDEYAEQQKKDGSIIVYTSMDSVLQIAAHEE", "aa_hexdigest": "6b70b7a38edd571932330ad7b6fe5ca5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8577.6579, "isoelectric_point": 4.5660696029663095 }, "id": "IGIGPGHMMC_1084", "locus": "GCA000148625_01058" }, { "type": "cds", "sequence": "contig_1", "start": 602129, "stop": 602446, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACGTGACTCAAAATTAGGTAAATTAAATAATGCTATGAAAGAAGACGACTTAATTAATTATAACAAGTGA", "aa": "MILNELQKAGVNVIGIGKIFDIFVGQGISESLHSDGDAHGMDLTIKCAEERPENTFVFTNLVQFDSHYGHRRDVDGYASNIALRDSKLGKLNNAMKEDDLINYNK", "aa_hexdigest": "5d8b62e379f4ef95e725578a0b7433df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11699.003799999986, "isoelectric_point": 5.200847434997558 }, "id": "IGIGPGHMMC_1085", "locus": "GCA000148625_01059" }, { "type": "cds", "sequence": "contig_1", "start": 602737, "stop": 603525, "strand": "+", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "GO:0003677", "GO:0043937", "SO:0001217", "UniRef:UniRef50_C4XDV1", "UniRef:UniRef90_C4XDV1" ], "nt": "ATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTCAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGA", "aa": "MNFTKFVKNEIINRKKNEVESLEYLRGLIFGNGLIKNDSIHLNIRDEEFFYLIIELIKKVNLDYQIKNSKTIVLNKKDIDLDVKFLKPSLFFGGVFQTGGSISNLGQTSYHLQLSSNYENFIDIIMSKLNEYYFNFQKLKHQNKYIIYVKQKEKIEDFLKAILALESFYKFTDITIKRDFENSFNRINNIDIYNIKKAVEANVKHIENLNYIFENKLENKFKDEQILLYKTVLNNPEAPLSNIILSLEKEHNLIKSKSTIHH", "aa_hexdigest": "4c106f292f1680bf729e239174251ee7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDV1", "query_cov": 1.0, "subject_cov": 0.9390681003584229, "identity": 0.996, "score": 505.0, "evalue": 1.84e-180, "valid": true, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_C4XDV1", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV1" ] }, "genes": [ "whiA" ], "id": "IGIGPGHMMC_1086", "locus": "GCA000148625_01060" }, { "type": "cds", "sequence": "contig_1", "start": 603576, "stop": 604034, "strand": "+", "frame": 3, "gene": null, "product": "DUF86 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV2", "UniRef:UniRef90_C4XDV2" ], "nt": "ATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATGAATTAATTCATTTCTTTTTTAAGGATATTGAATTTAAACAAACCGATGATCAATTAGCTTTATATCTTGATGAAAGAATTAATTTTTTAGAAGATCTTTTTTCAAGAACTAGACGATGA", "aa": "MKERMKYLNIKIQEKMNHIHYLTGTVVHSVQKLEYRLTYLITLKKTIDEYEKKGKFKHNLFLKVVLEASSEAQKIFRSLYENTFGNLSQKAYQNGILSKNEHLELSKVVEDRNELIHFFFKDIEFKQTDDQLALYLDERINFLEDLFSRTRR", "aa_hexdigest": "a3620180200999e4e1966a354983dd62", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XDV2", "query_cov": 1.0, "subject_cov": 0.9156626506024096, "identity": 1.0, "score": 295.0, "evalue": 9.02e-101, "valid": true, "product": "DUF86 domain-containing protein", "uniref50_id": "UniRef50_C4XDV2" }, "pscc": { "uniref50_id": "UniRef50_C4XDV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV2" ], "product": "DUF86 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1087", "locus": "GCA000148625_01061" }, { "type": "cds", "sequence": "contig_1", "start": 604126, "stop": 605091, "strand": "+", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_013354751.1", "SO:0001217", "UniParc:UPI0001E32EB0", "UniRef:UniRef100_UPI0001E32EB0", "UniRef:UniRef50_A0A3P8KAF3", "UniRef:UniRef90_C4XDV3" ], "nt": "ATGAATTTTATTTATGGCTCAGAAAAATTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAA", "aa": "MNFIYGSEKFFIEQEINKIVKKFPNSAITTFNIENDENVDSLIQFISSGDLFNKKRIIVINDLFYFEKKLSSNDETNVNNLIKSIDNNSSDELIFVNTNISNKEQINKNFFTNFIFNKLQQNNFIEANSIDDNQLTKKVVSLVQQKGGTIDALATSALLKKIPNDLYLINLEIDKLINQNKHITENMINVSVIDIYVEDAFGFSNSFETNDFNLIWKKYKEKLIEGVDVTVLIAQTSQLFILANQIYAYKNIGKTLENLASDFKINQYRVKKVSYLLTRLGTLKIRRMIKSLAKLDQDIKEGKTDPTIGFERFLIKFFING", "aa_hexdigest": "6a1250e1a47292d5748dec064b9aff8e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32EB0", "ncbi_nrp_id": "WP_013354751.1", "uniref100_id": "UniRef100_UPI0001E32EB0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EB0", "RefSeq:WP_013354751.1", "UniRef:UniRef100_UPI0001E32EB0" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32EB0", "uniref90_id": "UniRef90_C4XDV3" }, "psc": { "uniref90_id": "UniRef90_C4XDV3", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A3P8KAF3", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KAF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KAF3" ], "product": "DNA-directed DNA polymerase" }, "genes": [ "holA" ], "id": "IGIGPGHMMC_1088", "locus": "GCA000148625_01062" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 605841, "stop": 606113, "strand": "+", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 209.8, "evalue": 2e-22, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "AGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAAT", "id": "IGIGPGHMMC_43", "locus": "GCA000148625_01063" }, { "type": "cds", "sequence": "contig_1", "start": 606205, "stop": 606327, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354753.1", "SO:0001217", "UniParc:UPI0001E32EB1", "UniRef:UniRef100_A0AB32XC49", "UniRef:UniRef50_A0AB32XC49", "UniRef:UniRef90_A0AB32XC49" ], "nt": "ATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAA", "aa": "MSVIDKIKELIKENNGYMSTAILDKNKISRNYLKFLIEKN", "aa_hexdigest": "180a7bcc61b14991a91355f416da1c11", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32EB1", "ncbi_nrp_id": "WP_013354753.1", "uniref100_id": "UniRef100_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EB1", "RefSeq:WP_013354753.1", "UniRef:UniRef100_A0AB32XC49" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC49", "uniref90_id": "UniRef90_A0AB32XC49" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC49", "uniref50_id": "UniRef50_A0AB32XC49" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC49" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4716.521099999999, "isoelectric_point": 9.456109809875489 }, "id": "IGIGPGHMMC_1089", "locus": "GCA000148625_01064" }, { "type": "cds", "sequence": "contig_1", "start": 606584, "stop": 606784, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6", "UniRef:UniRef90_C4XDV6" ], "nt": "ATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAA", "aa": "MFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL", "aa_hexdigest": "9db485b1af58dc8b45d309d01aa42a9d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 606350, "stop": 606784, "observations": { "mutation": [ 606362, 606374, 606377, 606422, 606548 ], "stop": [ 606362, 606374, 606377, 606422, 606548 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDV6", "query_cov": 1.0, "subject_cov": 0.4583333333333333, "identity": 1.0, "score": 135.0, "evalue": 1.07e-39, "gene_start": 1, "gene_end": 66, "reference_start": 79, "reference_end": 144, "reference_sequence": "MCRYWYCRWWTLCISIEIQKVHFFWISAMFLQKITNVLYHRTEANFSTNYNTSKIDKKIKCHRIIDWKLNLGVEKVKIMFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL" }, "paralog": false, "identity": 0.9513888888888888, "score": 273.0, "evalue": 3.76e-96, "description": "Internal stop codon at 606362, 606374, 606377, 606422, 606548. Nonsense mutation around 606362, 606374, 606377, 606422, 606548.", "psc": { "uniref90_id": "UniRef90_C4XDV6", "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDV6", "UniRef:UniRef50_C4XDV6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7998.4307999999955, "isoelectric_point": 9.329042625427245 }, "id": "IGIGPGHMMC_1090", "locus": "GCA000148625_01065" }, { "type": "cds", "sequence": "contig_1", "start": 606936, "stop": 607823, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8", "UniRef:UniRef90_C4XDV7" ], "nt": "ATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTKYQVEASNYAFTTIEPNISSVPLKDPRLYELAKIVKPGRIVPATFDFVDIAGLVKGASKGEGLGNKFLGNIREVDAVVHVVRCFDDKNIMHVANEVNPVNDKDVINMELMLADLETINNIINRIHKKAKSGDKEAILEENLALKIKDLLENNKPARMIKNLSEDEEKLIKNYHLLTSKPMIYVANMSAEEIAEYENAPLYKQLKVSVEDDVKILPICVQLESEISQMDESESKEWLDSYNIKFSGLDLLAREAFDLLKLKTYFTAGEMEVKA", "aa_hexdigest": "bb607f6fba9700308db2b1169e322269", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XDV7", "query_cov": 1.0, "subject_cov": 0.8060109289617486, "identity": 1.0, "score": 565.0, "evalue": 4.06e-202, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_A0A3D5Z7W8", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D5Z7W8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "IGIGPGHMMC_1091", "locus": "GCA000148625_01066" }, { "type": "cds", "sequence": "contig_1", "start": 607839, "stop": 608036, "strand": "+", "frame": 3, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "nt": "ATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAA", "aa": "MLAPACAGIIHSDFEKKFIKADVISYQDYIDNNGELGARNAGKIRSEGKNYVMQDGDICHFKFGK", "aa_hexdigest": "332c967efb390a89ade3681ad845c738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378MX97", "query_cov": 0.9384615384615385, "subject_cov": 0.8591549295774648, "identity": 0.639, "score": 92.0, "evalue": 2.89e-23, "valid": false, "product": "GTP-dependent nucleic acid-binding protein engD", "uniref50_id": "UniRef50_A0A0P9MY34" }, "pscc": { "uniref50_id": "UniRef50_A0A0P9MY34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "IGIGPGHMMC_1092", "locus": "GCA000148625_01067" }, { "type": "tRNA", "sequence": "contig_1", "start": 608048, "stop": 608121, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 65.6, "nt": "AGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 608080, 608082 ], "id": "IGIGPGHMMC_13", "locus": "GCA000148625_01068" }, { "type": "cds", "sequence": "contig_1", "start": 608572, "stop": 608682, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACCTTTTACCTTATTTATTCTTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGA", "aa": "MFGPFTLFILLIVAAILLKIEIKENTRFATRVTGYR", "aa_hexdigest": "e6fc3e9fd6d0a2745f2da8b73e5ba784", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4157.017100000002, "isoelectric_point": 10.266412925720214 }, "id": "IGIGPGHMMC_1093", "locus": "GCA000148625_01069" }, { "type": "cds", "sequence": "contig_1", "start": 609431, "stop": 609748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTGATGGTGTTAATGATGCTCCCGCTCTTAAAACTAGTGACATTGGTTGTGCTATGGGAATTACAGGAACTGATGTTTCAAAACAAGCAGCTGATTTAATCTTAGTTGACGATAACTTCAATACTATTGTTAATTCAGTAAGAAATGGTCGTTTAATTTTTGACAAAATCAAAACAGTGATACTTAACTTATTAATTAGCTCACTAACAGAAATTTTAGTAATGCTTATTGGTATGGTCGCTTTCTTCTTAGCATATAAAGATTATTATCCAGATGGATTTTACATTTTTGGAGCTAGCCAATTATTATGA", "aa": "MTGDGVNDAPALKTSDIGCAMGITGTDVSKQAADLILVDDNFNTIVNSVRNGRLIFDKIKTVILNLLISSLTEILVMLIGMVAFFLAYKDYYPDGFYIFGASQLL", "aa_hexdigest": "6d492285e97b26a6143551e9cb2c73de", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11371.109499999991, "isoelectric_point": 4.355140113830567 }, "id": "IGIGPGHMMC_1094", "locus": "GCA000148625_01070" }, { "type": "cds", "sequence": "contig_1", "start": 609773, "stop": 610039, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGATTGATCCACCTCGGGCAGAGGTTGCAAATTCAATTAGAGAAGCTCAAGGTGCTGGTATTAAAGTTGTTATGATCACAGGTGATCAAGTAACTACAGCAAGAGCTATAGCTCAAAATTTAGGCATTTATCAAGAAAGCGATACTTGTCTTGAAGGCAAAGATTTAGCCAAAATGAGTGAAGAAGAATTAAGAGCTAAAGTTGAACATATTTCAGTTTATGCTCGTGTTAATCCTGAAGACAAATTAAGAATTGTTCAAGCATGA", "aa": "MIDPPRAEVANSIREAQGAGIKVVMITGDQVTTARAIAQNLGIYQESDTCLEGKDLAKMSEEELRAKVEHISVYARVNPEDKLRIVQA", "aa_hexdigest": "0a8369a165fe0e7449d5ec3d92cfbd6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 85, "aa_cov": 0.9431818181818182, "hmm_cov": 0.8117647058823529, "evalue": 6.9015571313348074e-09, "score": 32.37348937988281, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9651.904999999993, "isoelectric_point": 5.089272499084472 }, "id": "IGIGPGHMMC_1095", "locus": "GCA000148625_01071" }, { "type": "cds", "sequence": "contig_1", "start": 610145, "stop": 610294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTCGATTCAAATCGTAAAATGCACTCTGTAGTATTCAAAGAAAAAGATAGAATGCTTTTAGTTACTAAAGGTGCTCCTGATGTTTTATTAAAAAGATGTAATAATTTAACTATTGATGATATTAAAGAAATTAATCACCAATGA", "aa": "MPFDSNRKMHSVVFKEKDRMLLVTKGAPDVLLKRCNNLTIDDIKEINHQ", "aa_hexdigest": "5574b6ef4dc0134333c5777e7845c54c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5739.6747, "isoelectric_point": 8.978850364685059 }, "id": "IGIGPGHMMC_1096", "locus": "GCA000148625_01072" }, { "type": "cds", "sequence": "contig_1", "start": 610493, "stop": 610636, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAACAAAAAGGGTTGGTTAAAAACCTTTTAGCAGTTGAAACCCTAGGTTCTGCTTCAATTATTTGTAGTGATAAAACAGGAACACTAACAGAAAATAAAATGACAGTAGTTGATGCTTATCGTTTTAGAACTCTTTGA", "aa": "MSKQKGLVKNLLAVETLGSASIICSDKTGTLTENKMTVVDAYRFRTL", "aa_hexdigest": "7cff828fbd3b514d5e27dff5fb1ad487", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5132.972300000001, "isoelectric_point": 9.428839683532715 }, "id": "IGIGPGHMMC_1097", "locus": "GCA000148625_01073" }, { "type": "cds", "sequence": "contig_1", "start": 610835, "stop": 611581, "strand": "-", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "nt": "ATGAGCAAAAAAGACTATTCCAATTATCAAGGCTTGACTTCAAAAGAAGTTGAAGCAAACCTTGCAAAGTATGGTGAAAACGCTTTAATTAAAAAGAAAAAACTAAATCCAATAGTTGCTTTCTTTAGACAATTTGTTGATCCAATGGTTATTCTTTTAATCATAGCGGCAGCAATTAGTTTAACTTTGGCAATTGTTGAACATTTTCAAGGCGGAAAAAGCACAACCGATTTAATTATCTCTTATGTTGAACCTGGAATTATTATGCTAGTTATTATTTTAAATAGTATGTTAGGAGCTTATCAAGAGGTCAAAAGTGATCAAGCGGTGCGAGCCCTTGAAAAAGTTAATCAGGTCAATGCTACAGTAATTAGGGATGGTAAAGTCCAAATTATTCCCGCTAATTTATTAGTGCCTGGTGACTTAGTTGTTTTAGCTGCAGGAGATACAATTAATGCTGATGGTCGCTTAATTGAAGCTTCAAACTTAGAAGTTGTAGAAGCTTCATTAACTGGTGAAAGTTTACCAGTAAGTAAAGTCGCAAATTTAAATGCAACTGATGACAAAATCTTAGCTAACAACAAACACTTAGTTTACTCAGGCACTTATGTAATTAATGGACGGGCAACTTTTGTAGTTGAACATACAGCTAGTGAAACTGAAATTGGCAAAATTAATTCAATGATTCAAAAACAAAATAAAAATGCAACACCACTACAAATCAAACTTAATAAATTAAGCAAATGA", "aa": "MSKKDYSNYQGLTSKEVEANLAKYGENALIKKKKLNPIVAFFRQFVDPMVILLIIAAAISLTLAIVEHFQGGKSTTDLIISYVEPGIIMLVIILNSMLGAYQEVKSDQAVRALEKVNQVNATVIRDGKVQIIPANLLVPGDLVVLAAGDTINADGRLIEASNLEVVEASLTGESLPVSKVANLNATDDKILANNKHLVYSGTYVINGRATFVVEHTASETEIGKINSMIQKQNKNATPLQIKLNKLSK", "aa_hexdigest": "6d490fd2e04fe5e00203921f3a438294", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FFE00D", "query_cov": 0.9717741935483871, "subject_cov": 0.8515901060070671, "identity": 0.515, "score": 221.0, "evalue": 1.29e-68, "valid": false, "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI002FF3AF86" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF3AF86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "IGIGPGHMMC_1098", "locus": "GCA000148625_01074" }, { "type": "tRNA", "sequence": "contig_1", "start": 612013, "stop": 612104, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 73.9, "nt": "GGAACAGTACTCAAGTCGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGGCAACACCCGCGGAGGTTCGAATCCTCTCTGTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 612067, 612069 ], "id": "IGIGPGHMMC_31", "locus": "GCA000148625_01075" }, { "type": "cds", "sequence": "contig_1", "start": 612435, "stop": 612563, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATACCAACATAAAATTAGGAAATCAATCAACCGCTTTTAATGAAATACTAAAAGACCAATATTATAAAGCACTGTTAACTGACTCAGAACAACACAAAAGTTCATTGAGTAATCAAACATGA", "aa": "MGDTNIKLGNQSTAFNEILKDQYYKALLTDSEQHKSSLSNQT", "aa_hexdigest": "f56413d3ea013c24238cab15f7a14e79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4733.141100000001, "isoelectric_point": 5.498115348815917 }, "id": "IGIGPGHMMC_1099", "locus": "GCA000148625_01076" }, { "type": "cds", "sequence": "contig_1", "start": 612603, "stop": 612776, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTAAATTTAAAACTTTAGGACCAATTCAAAATAATTTCACTTTTGTTATAGATCATTTAGATAGTCCAGGAGCAGCAAAAAGTGGCGAATCAAAATCAAGTTTATTAAGTAAGCAAGGCGCTCAAGAAGCTGATGAAGCTTACAACTTAAAGAACCTTATGGATTGA", "aa": "MGVKFKTLGPIQNNFTFVIDHLDSPGAAKSGESKSSLLSKQGAQEADEAYNLKNLMD", "aa_hexdigest": "5532db168bd029edaecbddbe88526eef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6142.8354, "isoelectric_point": 5.641122245788575 }, "id": "IGIGPGHMMC_1100", "locus": "GCA000148625_01077" }, { "type": "cds", "sequence": "contig_1", "start": 612978, "stop": 613151, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGAATATTTTAAATTTATATGAAACATCTGAAGAATACAAAAAATATGGTTTAGCTAGTGTTATTGAACATTTAAATCTTGATGTTATTGGGCTTATTGAAAATAAAACAAAAGCTTCAGCACAAAAATTTTGTGAATACTTAAAAAAATTTACGGGAAACAACAATTGA", "aa": "MWNILNLYETSEEYKKYGLASVIEHLNLDVIGLIENKTKASAQKFCEYLKKFTGNNN", "aa_hexdigest": "f427d57dde27cf0904ea9bfeb67a583a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6628.495500000001, "isoelectric_point": 6.543952751159668 }, "id": "IGIGPGHMMC_1101", "locus": "GCA000148625_01078" }, { "type": "cds", "sequence": "contig_1", "start": 613953, "stop": 614120, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAACAATTTATTAAATTATTGGTAGGGTCTTTTTCTTTAATATCCTTACCTTTTTTATCAACTCAATGTAGCTTTTTTGAAATGAATAAAACACAAGAAAAAAACAAAGATGAAATTCAAAATGACTTAAATAATGCGGTTGAAAAAATCAAAATTTGA", "aa": "MKKKQFIKLLVGSFSLISLPFLSTQCSFFEMNKTQEKNKDEIQNDLNNAVEKIKI", "aa_hexdigest": "1d662c39a195fe31e3711c808814489d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6408.464800000002, "isoelectric_point": 9.252131843566893 }, "id": "IGIGPGHMMC_1102", "locus": "GCA000148625_01079" }, { "type": "cds", "sequence": "contig_1", "start": 614639, "stop": 615466, "strand": "-", "frame": 1, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80", "UniRef:UniRef90_C4XDW2" ], "nt": "ATGACAAAAATTATTCATAAAGAATTAGTGCCTATTCTTGATACACCTGTTATTGATAGATTAGTTAATGAAGCACTTGATTCAGGTATTGAGGAAATTTTAATTGTTTTAAGTGAAAGGAAAAAGGATTTATTAAAATTTTTCTCAGTTGATCAAGATTTACAAACAGAATTAATTATCAAAGGTAAAAAAGATATTTTAGAAAAAGTTAAAAGAACAAATCGCTCTTCATGTATTACTAGAGTAATTCAAAAGGAACAATTTGGTTTAGGTCATGCACTTGCAAGTTGTAAAGAATATATTGACAATGAGCCGTTTGCTATTATTTTAGGTGATGACTTAATTAAATCAAAAACTCCTGCTATTAAGCAATTAATCGAGTTTTATAATAAAACTGGAGCAAATGTTATTGGTGTACAATCAGTAGCAAAAGAATATATTCATAAATATGGAATTGTTAATCCAATAAACTTGAAAGATAAAGATAAGAAATATTTTGAAATTAATGGTGCAGTTGAAAAACCTAAGGCTGATAAAGCACCATCAAATAAAGCTATACTTGGACGTTATGTCTTTAATCCAGAAATTTTAGATATCTTATCTAGAATTGAATATGATGGCAAAAATGAAATTCAAGCAGTTGATGCATTTGATGAATTAATGAAAAAATATAAACAAAAAATTTATGCCTATGAATTTGAAGGCATCAGATATGATCTTGGATCAATGGAAGGCTTTGTTAAAGCAAACATTGATTATGCTTTAGAAGAACCTGAAATTGCAGACAAGATTAAAAGTTTTATTAATGAAAAAGTTAAAGAATTTTAA", "aa": "MTKIIHKELVPILDTPVIDRLVNEALDSGIEEILIVLSERKKDLLKFFSVDQDLQTELIIKGKKDILEKVKRTNRSSCITRVIQKEQFGLGHALASCKEYIDNEPFAIILGDDLIKSKTPAIKQLIEFYNKTGANVIGVQSVAKEYIHKYGIVNPINLKDKDKKYFEINGAVEKPKADKAPSNKAILGRYVFNPEILDILSRIEYDGKNEIQAVDAFDELMKKYKQKIYAYEFEGIRYDLGSMEGFVKANIDYALEEPEIADKIKSFINEKVKEF", "aa_hexdigest": "35ea3657a8e612087c2d43f66493c65d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_C4XDW2", "query_cov": 1.0, "subject_cov": 0.9105960264900662, "identity": 1.0, "score": 533.0, "evalue": 7.68e-191, "valid": true, "product": "UTP--glucose-1-phosphate uridylyltransferase", "uniref50_id": "UniRef50_W5UT80" }, "pscc": { "uniref50_id": "UniRef50_W5UT80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "IGIGPGHMMC_1103", "locus": "GCA000148625_01080" }, { "type": "tRNA", "sequence": "contig_1", "start": 615664, "stop": 615739, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 65.7, "nt": "GATCTTATAGCTCAATGGACAGAGTGTTGGGTTTCGGCCCCAAAGGTTAAGGGTTCGAGTCCCTTTAAGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 615704, 615706 ], "id": "IGIGPGHMMC_30", "locus": "GCA000148625_01081" }, { "type": "tRNA", "sequence": "contig_1", "start": 616762, "stop": 616837, "strand": "-", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 79.1, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCGCTGGTTCGATTCCTGCCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 616802, 616804 ], "id": "IGIGPGHMMC_29", "locus": "GCA000148625_01082" }, { "type": "tRNA", "sequence": "contig_1", "start": 616842, "stop": 616918, "strand": "-", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 75.8, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGTCTCCCTGTCACGGAGAAGATCGCGGGTTCAATTCCCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 616915, 616917 ], "id": "IGIGPGHMMC_28", "locus": "GCA000148625_01083" }, { "type": "tRNA", "sequence": "contig_1", "start": 616922, "stop": 616998, "strand": "-", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 72.3, "nt": "CGCGGAGTGGAGAAGTTTGGTATCTCGCGAGGCTCATAACCTCGAGGCCGCTGGTTCGAGTCCAGCCTCCGCCACCA", "db_xrefs": [ "SO:0000266" ], "id": "IGIGPGHMMC_27", "locus": "GCA000148625_01084" }, { "type": "tRNA", "sequence": "contig_1", "start": 617038, "stop": 617130, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 76.1, "nt": "GGGTGATTACCCAAGCCTGGCTGAAGGGACTAGTCTTGAAAACTAGCAGGGGCTTCACGGCCCGCGGGGGTTCGAATCCCTCATCACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 617125, 617127 ], "id": "IGIGPGHMMC_26", "locus": "GCA000148625_01085" }, { "type": "tRNA", "sequence": "contig_1", "start": 617132, "stop": 617206, "strand": "-", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 82.6, "nt": "GGACCTATAGCTCAACGGCAGAGCAACCGGCTCATAACCGGTTGGTTACTGGTTCGAATCCAGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "IGIGPGHMMC_25", "locus": "GCA000148625_01086" }, { "type": "tRNA", "sequence": "contig_1", "start": 617234, "stop": 617310, "strand": "-", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 78.8, "nt": "GGCGTTGTAGCTCAGTTGGTTAGAGCATCCGGTTCATACCCGGAAGGTCATGAGTTCGACTCTCATCGACGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 617283, 617285 ], "id": "IGIGPGHMMC_24", "locus": "GCA000148625_01087" }, { "type": "tRNA", "sequence": "contig_1", "start": 617316, "stop": 617392, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 83.0, "nt": "GGGGGATTAGCTCATTTGGCTAGAGCACTTGCCTTGCACGCAAGGGGTGGTGGGTTCGAATCCCATATCCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 617361, 617363 ], "id": "IGIGPGHMMC_23", "locus": "GCA000148625_01088" }, { "type": "tRNA", "sequence": "contig_1", "start": 617396, "stop": 617472, "strand": "-", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 81.9, "nt": "GGAGAAGTAGCTCAGCTTGGCAGAGCGCTACGTTTGGGACGTAGTGGTCGCAGGTTCAAATCCTGTCTTCTTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 617453, 617455 ], "id": "IGIGPGHMMC_22", "locus": "GCA000148625_01089" }, { "type": "cds", "sequence": "contig_1", "start": 617778, "stop": 618014, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTCGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGA", "aa": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDH", "aa_hexdigest": "d5e72f9b9cdf92903d1042cffd5882ee", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9370.73569999999, "isoelectric_point": 8.831411170959473 }, "id": "IGIGPGHMMC_1104", "locus": "GCA000148625_01090" }, { "type": "cds", "sequence": "contig_1", "start": 618054, "stop": 618818, "strand": "+", "frame": 3, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0", "UniRef:UniRef90_C4XDW6" ], "nt": "ATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGACGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATAAAGTGGCTTGA", "aa": "MVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHKVA", "aa_hexdigest": "abdd5f0424ae974091e29d7533e20e57", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 617778, "stop": 618851, "observations": { "mutation": [ 618012, 618039, 618816 ], "stop": [ 618012, 618039, 618816 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDW6", "query_cov": 1.0, "subject_cov": 0.711484593837535, "identity": 0.996, "score": 505.0, "evalue": 3.21e-179, "gene_start": 1, "gene_end": 254, "reference_start": 93, "reference_end": 346, "reference_sequence": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDHWYKLRIKKDWFDLSMVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHQVAWLKEFVEQFKD" }, "paralog": false, "identity": 0.988795518207283, "score": 702.0, "evalue": 2.07e-259, "description": "Internal stop codon at 618012, 618039, 618816. Nonsense mutation around 618012, 618039, 618816.", "psc": { "uniref90_id": "UniRef90_C4XDW6", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDW6", "UniRef:UniRef50_A0A3P8L7N0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0" ], "product": "Haloacid dehalogenase-like hydrolase" } }, "id": "IGIGPGHMMC_1105", "locus": "GCA000148625_01091" }, { "type": "rRNA", "sequence": "contig_1", "start": 619010, "stop": 621893, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9859829059829059, "score": 2779.2, "evalue": 5.9e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TTTTAAATAAGGAAGAGTTTGTGGTGGATGCCTTGGGTCTGAAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGTGGAGCCGGAAATATGCTATGAAACGGGGATTTCCGAATGGGGAAACCTAACTAGGGTAATGCCTAGTTGCCTTGTTCTGAATTCATAGGAACTTGAGTGAGACACCTTGTGAACTGAAACATCTTAGTAGCAAGAGGAAGAGAAAATAAACAATGATTTCTTTAGTAGCGGCGAGCGAACGAGAAAGAGCCCAAACCCAGCAATGGGGGTTGTAGGACGATCTACATGAAGTTACAAAACTTAATGATAGCAGAAGCTTTTGGGAAGAAGCGGCATAGAGGGTGATACCCCCGTATGTGAAATTGTTAAGTCTTCTGATCGTATCCTGAGTAGGGCGGGGCACGTGGAACCCTGTCTGAATCTGCCGGGACCACCCGGTAAGGCTAAATACTAATCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGAGAGGGGAGTGAAATAGATTCTGAAACCACTTACTTACAATTAGTCAGAGCCCGTTTATGGGTGATGGCGTACATCTTGCAGTATGGACCGGCGAGTTATGACAGCATGCGAGGTTAAGCAGAAAAAAGCGGAGCCGTAGAGAAATCGAGTCTTAATAGGGCGTCTTAGTATGTTGTCATATACCCGAAACCATGTGATCTATTCATGAGCAGGCTGAAACTTGGCTAACCCCAAGCGGAGGGCCGAACCGTAGTACGCTGAAAAGTGCCCGGATGACTTGTGAATAGTGGAGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCCTCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAGTAGTGGTGGTAGAGCACTGAATATGGAATGGCCTCGCCTAGGGGTACTGACTATAATCAAACTCCGAATACCGCTATGTATTGCTATGCAGTCGGAACCGGGGTGCTAACGTCCCGGCTCGCGAGGGCAACAACCCAGATCGCCGGCTAAGGTCCCAAAATCGTGTTAAGTGAGAAAGGTTGTGAGGTTTCATAAACAACTAGGAAGTTGGCTCAGAAGCAGCCACCTTTTAAAGAGTGCGTAATAGCTCACTAGTCAAGAGACCTTGCGCCAACAATTTAACGGGACTAAAACACGATACCGAAGCCGCGGGTAATTATATTTACGTTAGAGGAGCGTTCCTAGGGCAATGAAGTCAGACCGTGAGGACTGGTGGAGCGCTAGGAAGTGAGAATGCCGGTATGAGTAACGATTCGTAGTGAGAATCTACGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGACCTAAGACGAGGCTGAAAAGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTATAATAGTGATGGAGTGACGGAGGAGGATAATCTTACCACTTACTGGATTGTGGGGTAAGCGTTAACTGGTGACTGTAGGCAAATCCGCAGTCTATAACCGGGAGGCGTGATGCATAGGGTCAAACCGAATTAGATAATTCCACACTTCCAAGAAAAGCTTCTAAACTTAATATTATGGAAACCTGTACCGAGAACGGACACACGTCCCCGAGATGAGTATTCTAAGGCGAGCGAGAAAACTAGTGTTAAGGAACTCTGCAAAATGACCCCGTAAGTTCGCAAGAAGGGGTGCCTAAGGTAACACTTAGGCCACAGTAAATTATGAGGGGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAACCGCAAGGTGATGTATAGGGGGTGAAGCCTGCCCAGTGCCCGAAGGTTAAGTGGATGCGTTAGCATTTGCGAAGCGTTGAAATGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGCGCAATGATCTCTCAACTGTCTCAACACTAGACTCGGTGAAATTATGGTCCCAGTGAAAACGCTGGGTACCCGCATCAAGACGAAAAGACCCCATGGAGCTTTACTACAGTTTCGTATTGGAACTTGGTCTAACATGTGTAGGATAGGTGGGAGACTTTGAAGCGAGGACGCTAGTTCTCGTGGAGTCATCCTTGAAATACCACCCTTGGTATATTGAGTTTCTAACATGCCATCATCATCAGGTGGGTGGACAGTGCGTGACGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGAGTAACGGAGGCGTTCAAAGGTACACTCAGTACGGTCAGAAACCGTATGTAGAGCGCAAAGGTAGAAGTGTGCTTGACTGTGAGACTTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGCTGACCAATACTAATAAATCGATCGGTTTAAAAGT", "id": "IGIGPGHMMC_41", "locus": "GCA000148625_01092" }, { "type": "rRNA", "sequence": "contig_1", "start": 622207, "stop": 623719, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9869536855838226, "score": 1507.4, "evalue": 4.8e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGGCGTTAAATTTTGGGGCTCAACCCCAAAACGCGTTGGATACTGGCAGGCTAGAGTTGTGTAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAAGATGGCGAAGGCAGCTAACTGGACATATACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATCATTAGCTGATGGGGAACTCATCGGCGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTACGTTCGCAAGAATAAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATGGAGACATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCTGCAACGAGCGCAACCCTTATCCTTAGTTACTACCATTTAGTTGAGGACTCTAAGGAGACTGCCCGAGTAATCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCCGGTACAAAGAGAAGCGAAGTGGTGACATGGAGCAAACCTCAAAAAACCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTATAAACAAACTGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "IGIGPGHMMC_39", "locus": "GCA000148625_01093" }, { "type": "cds", "sequence": "contig_1", "start": 624043, "stop": 624165, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00828.25" ], "nt": "ATGCCTGTTAAATTATTGGGTAATGGTCAAGTAACTAAAAAATTCAATGTTAATCTTCATGCAGCTTCAAAAAGTGCTATTGATGCAATCGAAGCTAAAGGTGGAAAAGTTACTTTATTATAG", "aa": "MPVKLLGNGQVTKKFNVNLHAASKSAIDAIEAKGGKVTLL", "aa_hexdigest": "c78cf49d5fc03939e8b08fbbd696d387", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00828.25", "name": "Ribosomal_L27A", "length": 37, "aa_cov": 0.925, "hmm_cov": 1.0, "evalue": 8.444635393863683e-15, "score": 51.03211212158203, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4162.896099999999, "isoelectric_point": 10.000287437438963 }, "id": "IGIGPGHMMC_1106", "locus": "GCA000148625_01094" }, { "type": "cds", "sequence": "contig_1", "start": 624313, "stop": 624483, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCAACATTACAATCATTAAGACCTACCGCTGGCTCACGTGTTGAAAAACACCGTGTTGGTCGCGGACATGCTGCTGGAAAAGGTAAACAAGCTGGTAAAGGTCAAAGTGGACAAAACAAACGTAAGGGTCACAGACTAGGATTTGAAGGTGGTCAAACACCTTGA", "aa": "MNSTLQSLRPTAGSRVEKHRVGRGHAAGKGKQAGKGQSGQNKRKGHRLGFEGGQTP", "aa_hexdigest": "dfca84a96eb9aa8097633e2899427aab", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5893.543500000002, "isoelectric_point": 11.999967765808105 }, "id": "IGIGPGHMMC_1107", "locus": "GCA000148625_01095" }, { "type": "cds", "sequence": "contig_1", "start": 624538, "stop": 624756, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03719.21" ], "nt": "ATGCTTAAACCTGCTACAAAAGGTAAAGGTATTGTTGCTAGTGGTACAGTTAGAGCAGTTGTTGAACTTGCAGGTTACACCGATATTTATACCAAAACTTATGGTTCACGTTCAAAAGCAAACATTGTTAGAGCAACTTTAAAAGCTCTTCAAGCTTTAAGAACACCAGAACAAATTGCTGACATTCGTGGTAAAGATGTTAAAGATCTTTTAGCCTAA", "aa": "MLKPATKGKGIVASGTVRAVVELAGYTDIYTKTYGSRSKANIVRATLKALQALRTPEQIADIRGKDVKDLLA", "aa_hexdigest": "28fc6c4456239668fbf572f76a0c66b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03719.21", "name": "Ribosomal_S5_C", "length": 63, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 1.510222522940169e-26, "score": 87.71133422851562, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 7702.929999999999, "isoelectric_point": 10.070106697082519 }, "id": "IGIGPGHMMC_1108", "locus": "GCA000148625_01096" }, { "type": "cds", "sequence": "contig_1", "start": 625123, "stop": 625248, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAGAAGTAAAGAAAGTTGTTAATAAAGAGCAACCTAAAGAAGCCCAAGCTCAAGGAACTAAAGAAGTTGTAGCTCCTAAAGCTAAAGCTGAAAACAAAGATAAAAAACAAATTTGA", "aa": "MAEAEVKKVVNKEQPKEAQAQGTKEVVAPKAKAENKDKKQI", "aa_hexdigest": "4287dce5a67b631dc5ca2eaa847fb5dc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4520.171200000001, "isoelectric_point": 9.399055290222169 }, "id": "IGIGPGHMMC_1109", "locus": "GCA000148625_01097" }, { "type": "cds", "sequence": "contig_1", "start": 625251, "stop": 625616, "strand": "-", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354765.1", "SO:0001217", "UniParc:UPI0001E32EBC", "UniRef:UniRef100_C4XFZ9", "UniRef:UniRef50_Q4A5D7", "UniRef:UniRef90_A0A3P8LIJ1" ], "nt": "ATGGCTACATTATCTCGTAATCAAGCACGTCAAGTTAAACACTTGCGTGAACGTCAAAGTATTTCAGGTACTGCTACTAAACCACGTCTTTGCGTATTCAAATCTCATACAAACTTTTATGCACAACTAATTGACGATACAACAGGACACACTTTGGCAAGTGTTTCAACACTTGATCCTAAAGCTAAAGTTAAATACCACGGAAACATTGCTTCAGCAACTAAAGCTGGTGAAGCAATGGGTGCTAAAATTGCAGCCCTTAAATTAAAAGAAATAGTATTCGATCGTTCAGGTTACATTTATCATGGACGTGTTAAAGCCTTCGCAGAAGGTGTTAGAAGTATAGCTAAAGGAGTTAAATTCTAA", "aa": "MATLSRNQARQVKHLRERQSISGTATKPRLCVFKSHTNFYAQLIDDTTGHTLASVSTLDPKAKVKYHGNIASATKAGEAMGAKIAALKLKEIVFDRSGYIYHGRVKAFAEGVRSIAKGVKF", "aa_hexdigest": "58b59e311212f3d90497aedf3886588a", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EBC", "ncbi_nrp_id": "WP_013354765.1", "uniref100_id": "UniRef100_C4XFZ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EBC", "RefSeq:WP_013354765.1", "UniRef:UniRef100_C4XFZ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ9", "uniref90_id": "UniRef90_A0A3P8LIJ1" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LIJ1", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q4A5D7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D7" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "IGIGPGHMMC_1110", "locus": "GCA000148625_01098" }, { "type": "cds", "sequence": "contig_1", "start": 625643, "stop": 626182, "strand": "-", "frame": 1, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_013354766.1", "SO:0001217", "UniParc:UPI0001E32EBD", "UniRef:UniRef100_C4XG00", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_C4XG00" ], "nt": "ATGTCTCGTGTTGGAAATAGAGTATTAAATATCCCTGCAGGTGTTGAAGTTAATTTAAATGGTACAACCTTAAATGTTAAAGGTTCATTAGGCCAATTAGAAAAAACATTCAGCCCACTTATTGCAATTAAAATTGAAAATAATCAAATTACAACCCTCCGTGCTAATGAAGAAAAACACACAAAACAATTACACGGAACAACAAATGCTTTAATTCAAGGCATGCTTGAAGGTGTTTCAAAAGGTTTCAAAAAAGAATTAGCAATCAAAGGTGTTGGTTACAAAGCTGCACTTAATGGTCAAACTTTAGAATTAACTGTTGGTTACAGCCACATAGTTAGTCTAAGTGTACCAGAAGGTGTTAAAGTTGAAGTTCCAAAACCTCTTGAAATCATTATTAGTGGTATTGACAAAGAAAAAGTTGGTCAATTTGCTGCTGTTGTGCATGATGTTCGTCGTCCTAACTCATACTCAGGTAAAGGGATTTCATACAAAGGCGAATACATTCGTATTAAAGAAGGAAAAACTGCTTCTAAATAG", "aa": "MSRVGNRVLNIPAGVEVNLNGTTLNVKGSLGQLEKTFSPLIAIKIENNQITTLRANEEKHTKQLHGTTNALIQGMLEGVSKGFKKELAIKGVGYKAALNGQTLELTVGYSHIVSLSVPEGVKVEVPKPLEIIISGIDKEKVGQFAAVVHDVRRPNSYSGKGISYKGEYIRIKEGKTASK", "aa_hexdigest": "1d3b64968fc4c18bc8af2cf04abb7e59", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E32EBD", "ncbi_nrp_id": "WP_013354766.1", "uniref100_id": "UniRef100_C4XG00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EBD", "RefSeq:WP_013354766.1", "UniRef:UniRef100_C4XG00" ] }, "ips": { "uniref100_id": "UniRef100_C4XG00", "uniref90_id": "UniRef90_C4XG00" }, "psc": { "uniref90_id": "UniRef90_C4XG00", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "IGIGPGHMMC_1111", "locus": "GCA000148625_01099" }, { "type": "cds", "sequence": "contig_1", "start": 626197, "stop": 626589, "strand": "-", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_C4XG01" ], "nt": "ATGTTTATCACAGATCCAATTTCAGATATGATTGTTAGAATCAAAAACGCTAATCAACGTAAATTCAAAACTGTAGCTATTCCTTTTTCAAATAAAAAAGCTAAAATTCTAGAAATTCTTCAAAATGAAGGTTACATTACAAACTTTGTTACAAAAGGCCAAGGTAAAGACAAAGTACTTGAAGTTTCATTAAAATACAAAAACAACCAAAGTGCAATTGTTGACTTTAAACGTGTTTCAAAACCAGGTCTTAAAGTTTATACTTCAGCTGACAAATTACCTACCATTTTATCAGGTTATGGTACAGCAATCATTTCAACTTCAAAAGGAATGATGACTGAAAAACAAGCTCGTAAGGAAAATGTGGGTGGTGAAATCATTGCCTACATTTGA", "aa": "MFITDPISDMIVRIKNANQRKFKTVAIPFSNKKAKILEILQNEGYITNFVTKGQGKDKVLEVSLKYKNNQSAIVDFKRVSKPGLKVYTSADKLPTILSGYGTAIISTSKGMMTEKQARKENVGGEIIAYI", "aa_hexdigest": "a5856a56c4197a0d5098d6f8d5af4378", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XG01", "query_cov": 1.0, "subject_cov": 0.9701492537313433, "identity": 1.0, "score": 250.0, "evalue": 4.25e-84, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "IGIGPGHMMC_1112", "locus": "GCA000148625_01100" }, { "type": "cds", "sequence": "contig_1", "start": 626595, "stop": 626777, "strand": "-", "frame": 3, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_A5IYX3", "UniRef:UniRef90_A5IYX3" ], "nt": "ATGGCTAAAGTTTCATTAAAAGCAAAACAAAAGAAACATCCTAAATTCTCAACACGTGCTTACACACGTTGTGAATTATGTGGACGTCCACACGCAGTTTTAAGAAAATACAAGATTTGCCGTATTTGTTTCCGTAATTTAGCTCATGAAGGAAAAATTCCTGGCATGAAGAAAGCGAGTTGA", "aa": "MAKVSLKAKQKKHPKFSTRAYTRCELCGRPHAVLRKYKICRICFRNLAHEGKIPGMKKAS", "aa_hexdigest": "d8ef3eb6eb98730ca40562b821f7b0c7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A5IYX3", "query_cov": 1.0, "subject_cov": 0.8823529411764706, "identity": 1.0, "score": 127.0, "evalue": 2.79e-37, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_A5IYX3", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX3" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [ "rpsZ" ], "id": "IGIGPGHMMC_1113", "locus": "GCA000148625_01101" }, { "type": "cds", "sequence": "contig_1", "start": 626779, "stop": 627006, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "nt": "ATGCCACGTATTCGTGATTTCCGTGGTGCCAACCCTAAAGCATTTGATGGTAGAGGAAACTACTCATTAGGTGTTAAAGAAGAAATTATTTTCCCAGAAATCGAATTTGACAAAATTCGTCACATCAAGGGACTTGATGTTCAATTAATCACAAGCACAAATAAAGATGAAGAAGCTAGAAGTTTATTAGAATTAATCGGCATTCCATTTATTAAAGGAGATAGATAA", "aa": "MPRIRDFRGANPKAFDGRGNYSLGVKEEIIFPEIEFDKIRHIKGLDVQLITSTNKDEEARSLLELIGIPFIKGDR", "aa_hexdigest": "e529e4da82ed9014b0ac75de865e36ee", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NY94", "query_cov": 0.9866666666666667, "subject_cov": 0.9866666666666667, "identity": 0.595, "score": 104.0, "evalue": 6.4e-28, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A3B0NY94", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NY94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "IGIGPGHMMC_1114", "locus": "GCA000148625_01102" }, { "type": "cds", "sequence": "contig_1", "start": 627097, "stop": 627324, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGAATTTAAAAGCTTATTACACAAAAACAGTTGTACCTGCATTAATGAAAAAATATAACTATTCATCAGTTATGCAAGTACCACGTTTAGAAAAAATTGTTTTGAATATGACAGCAGGTAAAGAAGTAACAAACTCTAAAGCTATTGAAGAAGTTCTTAATGAACTTACTCAAATTTCAGGTCAAAAACCATTCCAAACCAGAGCTAAAAAATCAAATGCTTCATGA", "aa": "MNLKAYYTKTVVPALMKKYNYSSVMQVPRLEKIVLNMTAGKEVTNSKAIEEVLNELTQISGQKPFQTRAKKSNAS", "aa_hexdigest": "fa8b97cf1baa8f1c4b7da190658b4a16", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.9333333333333333, "subject_cov": 0.8333333333333334, "identity": 0.586, "score": 83.6, "evalue": 1.39e-19, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "IGIGPGHMMC_1115", "locus": "GCA000148625_01103" }, { "type": "cds", "sequence": "contig_1", "start": 627327, "stop": 627653, "strand": "-", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354770.1", "SO:0001217", "UniParc:UPI0001E32EC1", "UniRef:UniRef100_C4XG04", "UniRef:UniRef50_Q98PZ3", "UniRef:UniRef90_C4XG04" ], "nt": "ATGAAATTAAAACTTAAGAAGAATGACGAAGTTATTGTTATTGCAGGAAGCAATAAAGGTAAAATTGGTCGTATTATTAGAACAAATTTTAAAACAAACCGTGCATATGTAAAAGATGTAAACGTAGTAACTAAACACGTAAAACCAAGTCAAAGTAATACAGAAGGTTCAATCAAGAACGTTGAAGCTCCTATTCACATTTCAAATCTTGCAATCGTTACTAAAAAAGCTACCAAAAGTGGTGCTGCTCAATATTCAAAAGTTGGTTTTAAAGTTAAAGGTAACACTAAAGTTAGAGTTGCTCGTAAAACTAAGAAGGAAATTTAA", "aa": "MKLKLKKNDEVIVIAGSNKGKIGRIIRTNFKTNRAYVKDVNVVTKHVKPSQSNTEGSIKNVEAPIHISNLAIVTKKATKSGAAQYSKVGFKVKGNTKVRVARKTKKEI", "aa_hexdigest": "3e73b247f84e4d4d23a189070ede1090", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001E32EC1", "ncbi_nrp_id": "WP_013354770.1", "uniref100_id": "UniRef100_C4XG04", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC1", "RefSeq:WP_013354770.1", "UniRef:UniRef100_C4XG04" ] }, "ips": { "uniref100_id": "UniRef100_C4XG04", "uniref90_id": "UniRef90_C4XG04" }, "psc": { "uniref90_id": "UniRef90_C4XG04", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_Q98PZ3", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ3" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "IGIGPGHMMC_1116", "locus": "GCA000148625_01104" }, { "type": "cds", "sequence": "contig_1", "start": 627675, "stop": 628043, "strand": "-", "frame": 3, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_013354771.1", "SO:0001217", "UniParc:UPI0001E32EC2", "UniRef:UniRef100_A0AB32XC52", "UniRef:UniRef50_Q85CT4", "UniRef:UniRef90_C4XG05" ], "nt": "ATGGTTATAGCACTTTCTAAATTAAATGTTGCAGATAACTCTGGAGCAAAAGAAGTTGGTGTAATCAAAGTTTTAGGTGGAAGTAGAAAGAAAACAGCTAATATCGGTGACGTTATTATCTGTTCAGTTAAAAAAGCTTTACCTAATGGTTTAGTAAAAGAAGGACAAGTAGTTAAAGGTGTTATTGTTAGAAGTGTTTATGGAATTAGCCGTGAAAATGGTACACATATTCGTTTCGACGATAATGCAGTTGTGTTAATTAAAGATGATGGTACTTTAAGAGGTACTCGTGTGTTTGGACCTGTTGCTCGTGAATTACGTGACCGTGGTTATTTAAAAATCGTTTCATTAGCACCTGAAGTTCTTTAA", "aa": "MVIALSKLNVADNSGAKEVGVIKVLGGSRKKTANIGDVIICSVKKALPNGLVKEGQVVKGVIVRSVYGISRENGTHIRFDDNAVVLIKDDGTLRGTRVFGPVARELRDRGYLKIVSLAPEVL", "aa_hexdigest": "e3333d702fd7dd8deb44c64a446d5a98", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32EC2", "ncbi_nrp_id": "WP_013354771.1", "uniref100_id": "UniRef100_A0AB32XC52", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC2", "RefSeq:WP_013354771.1", "UniRef:UniRef100_A0AB32XC52" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC52", "uniref90_id": "UniRef90_C4XG05" }, "psc": { "uniref90_id": "UniRef90_C4XG05", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q85CT4", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "genes": [ "rplN" ], "id": "IGIGPGHMMC_1117", "locus": "GCA000148625_01105" }, { "type": "cds", "sequence": "contig_1", "start": 628043, "stop": 628309, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354772.1", "SO:0001217", "UniParc:UPI0001E32EC3", "UniRef:UniRef100_C4XG06", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_C4XG06" ], "nt": "ATGGAAAGAAGTTCACGTAAGACCCTTCAAGGTCGTGTTGCTTCAGCACGTGGTGATAAAACTATCATCGTTGAAGTTGAAAGTTATAAAGCTCATAGTCTTTACTCAAAACGTTTTAAAGTTGTTAAACGTTTCGCAGTTCATGATGAAAAAAACTTAGCTAAAGTTAACGACATCGTTACAATTATGGAAACACGTCCTCTTTCAAAAACCAAACACTTCCGTCTTGTTGAAATTAAACAACACGCAGTTGAAGGAGAAAAATAA", "aa": "MERSSRKTLQGRVASARGDKTIIVEVESYKAHSLYSKRFKVVKRFAVHDEKNLAKVNDIVTIMETRPLSKTKHFRLVEIKQHAVEGEK", "aa_hexdigest": "9988ace3ccb6a0572c22a13f19cba37f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC3", "ncbi_nrp_id": "WP_013354772.1", "uniref100_id": "UniRef100_C4XG06", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC3", "RefSeq:WP_013354772.1", "UniRef:UniRef100_C4XG06" ] }, "ips": { "uniref100_id": "UniRef100_C4XG06", "uniref90_id": "UniRef90_C4XG06" }, "psc": { "uniref90_id": "UniRef90_C4XG06", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "IGIGPGHMMC_1118", "locus": "GCA000148625_01106" }, { "type": "cds", "sequence": "contig_1", "start": 628309, "stop": 628512, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354773.1", "SO:0001217", "UniParc:UPI0001E32EC4", "UniRef:UniRef100_C4XG07", "UniRef:UniRef50_A5IYX8", "UniRef:UniRef90_C4XG07" ], "nt": "ATGCTTTATAAAGATATTAAAGTTAAAAATAATGCTGAACTCCGTAAATTACTTGACGATTTAAAAGCACAATTATTTATTTATCGTTTCCAAAACAAAACTGGGACATTAGATCACAGTCACAAAATTAGAGAAGTACGTCGTGACATTGCTAAAGTATTAACAGTTTTAAAAATTAACGAATCTAAAGGAGGAGCTAAATAA", "aa": "MLYKDIKVKNNAELRKLLDDLKAQLFIYRFQNKTGTLDHSHKIREVRRDIAKVLTVLKINESKGGAK", "aa_hexdigest": "37e9c9e6d745504fa848d9e6f84a8d70", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC4", "ncbi_nrp_id": "WP_013354773.1", "uniref100_id": "UniRef100_C4XG07", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC4", "RefSeq:WP_013354773.1", "UniRef:UniRef100_C4XG07" ] }, "ips": { "uniref100_id": "UniRef100_C4XG07", "uniref90_id": "UniRef90_C4XG07" }, "psc": { "uniref90_id": "UniRef90_C4XG07", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A5IYX8", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX8" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "IGIGPGHMMC_1119", "locus": "GCA000148625_01107" }, { "type": "cds", "sequence": "contig_1", "start": 628656, "stop": 628763, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGTCGTGAAGGTCAAGTTTTCATTAGAATCTTCCCTCACTTCTCAAAAACATCAAAACCTATCGGTGTTCGTATGGGTTCTGGTAAAGGTAGTCCTGAAAAATGA", "aa": "MGREGQVFIRIFPHFSKTSKPIGVRMGSGKGSPEK", "aa_hexdigest": "697d90d1d37e401998077d88652ea801", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 2.534671389686737e-10, "score": 36.4363899230957, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3847.4728000000005, "isoelectric_point": 11.068786430358884 }, "id": "IGIGPGHMMC_1120", "locus": "GCA000148625_01108" }, { "type": "cds", "sequence": "contig_1", "start": 628812, "stop": 628937, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAATATAGAAAACCATTTTTAGTACGTCATGACAAACGTAAAGCCTTCAAAGGTAATACAGTTGCTTATGGTGACTTTGGTTTACAAGCTGTAAGCTCAGCTTGA", "aa": "MLQPKRTKYRKPFLVRHDKRKAFKGNTVAYGDFGLQAVSSA", "aa_hexdigest": "a28e4943ebc9e731e4abe2f6b2bfc039", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.454900000001, "isoelectric_point": 10.74824962615967 }, "id": "IGIGPGHMMC_1121", "locus": "GCA000148625_01109" }, { "type": "cds", "sequence": "contig_1", "start": 628975, "stop": 629094, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "ATGGCTAGAACAGAAGGCTATTCACGTGGAGAAATGAGACTTCACACATTAAGACAAGATGTTGATTATGCACACGCAAATGCTCACACTACTTATGGTGTAATCGGCGTTAAAGTGTGA", "aa": "MARTEGYSRGEMRLHTLRQDVDYAHANAHTTYGVIGVKV", "aa_hexdigest": "6bec5ed16b8584a907b907b62f925a05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 39, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 4.129403222990602e-15, "score": 52.15079879760742, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4404.901, "isoelectric_point": 8.247198677062986 }, "id": "IGIGPGHMMC_1122", "locus": "GCA000148625_01110" }, { "type": "cds", "sequence": "contig_1", "start": 629596, "stop": 629955, "strand": "-", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02890", "RefSeq:WP_013354776.1", "SO:0001217", "UniParc:UPI0001E32EC7", "UniRef:UniRef100_C4XG10", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_C4XG10" ], "nt": "ATGGCACAAAATACACCAGTTGTTCAACAAGCTAAAGCTCATGTTGAAATTCAACGTGTTAGTGCACGTAAAGCACGTTTAGTTGCTGACTTAGTTAGATCTAAAGACGTGCGTGAAGCTCTTGGTATTTTAAGAAATACAAACAAGAAATCTTCAGAATTATTTATTAAACTTATTAACTCAGCAATTGCTAATGCTACAAACAACCACGGAATGGATGCATCTAAATTATTTGTTAAAGAAATCTTAGTTAATGAAGGACCAACACTTAAAAGATACCAACCTCATTCAAGAGGAACAGCTTATTCAATTTTTAAACGTACATCACATTTATCAATTACATTAGAAGAAAGAAAGTAG", "aa": "MAQNTPVVQQAKAHVEIQRVSARKARLVADLVRSKDVREALGILRNTNKKSSELFIKLINSAIANATNNHGMDASKLFVKEILVNEGPTLKRYQPHSRGTAYSIFKRTSHLSITLEERK", "aa_hexdigest": "eb44942af9594e8c854873f987f7fb42", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32EC7", "ncbi_nrp_id": "WP_013354776.1", "uniref100_id": "UniRef100_C4XG10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC7", "RefSeq:WP_013354776.1", "UniRef:UniRef100_C4XG10" ] }, "ips": { "uniref100_id": "UniRef100_C4XG10", "uniref90_id": "UniRef90_C4XG10" }, "psc": { "uniref90_id": "UniRef90_C4XG10", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "IGIGPGHMMC_1123", "locus": "GCA000148625_01111" }, { "type": "cds", "sequence": "contig_1", "start": 630129, "stop": 630233, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAGTCTTAAAAAAGGACCTTTTGCAGACGAACATTTACTTAAAAAAGTAGATGCTATTGTTGAAGGCAAAGCGCCAAAAAAACCAATTAAAACTTGA", "aa": "MARSLKKGPFADEHLLKKVDAIVEGKAPKKPIKT", "aa_hexdigest": "159a49d0b0c41ac497ccc03212c29f2d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.4823000000015, "isoelectric_point": 9.998095512390137 }, "id": "IGIGPGHMMC_1124", "locus": "GCA000148625_01112" }, { "type": "cds", "sequence": "contig_1", "start": 630320, "stop": 631078, "strand": "-", "frame": 1, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_D5E4V9" ], "nt": "ATGGCAATTAAACATTACAAGCCAACTACGAATGGACGTCGTAATATGTCATCTCTACATTACACAGAAAACTTAAGTGGACATGCTCCTGAAAAATCTTTACTTACACTATTAAAATATAGTGCTGGTCGCAATAACCAAGGTAAGATTACAGTACGTCACAAGGGTGGTCGTGTTAAGAGATTTTACAGATTAATTGACTTCAAACGTAATAAGGACAATATTCCAGCAGTAGTTAAGACCATTGAATATGATCCTAACCGTTCAGCTAATATTTGTTTATTAGCATATGCTGATGGAGAAAAAAGGTATATTTTAGCGCCTGAAGGCATTAAAGTAGGCCAAAGCGTTATTTCAGGTGAACACGCTGACATTTTAGTAGGTAATGCATTACCACTTTCAAGCATTCCAGAAGGTACAATTGTACACAACATTGAAATGCAACCAGGTGGTGGAGGTATTATTGCTAGATCAGCAGGTACATCTGCACAAATCTTAGGTAAAGATGACGACGGTAAATATGTTGTTTTACGTCTAAAAAGCGGTGAAATGCGTCGTATCTTAGCTCGTTGCCGTGCAACTATCGGTTCAGTTGGTAACGAAGAACATTTATTGGTTGATTTAGGAAAAGCTGGTAGAAACCGTTATAAAGGTGTTCGTCCAACAGTTCGTGGTTCAGTTATGAACCCTGTGGATCACCCACATGGTGGTGGTGAAGGTAAACAACCTGTTGGTCGTAAAGCTCCTCTTACTCCTTGA", "aa": "MAIKHYKPTTNGRRNMSSLHYTENLSGHAPEKSLLTLLKYSAGRNNQGKITVRHKGGRVKRFYRLIDFKRNKDNIPAVVKTIEYDPNRSANICLLAYADGEKRYILAPEGIKVGQSVISGEHADILVGNALPLSSIPEGTIVHNIEMQPGGGGIIARSAGTSAQILGKDDDGKYVVLRLKSGEMRRILARCRATIGSVGNEEHLLVDLGKAGRNRYKGVRPTVRGSVMNPVDHPHGGGEGKQPVGRKAPLTP", "aa_hexdigest": "054fb9374afee5cadbbdf737004e8920", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D5E4V9", "query_cov": 1.0, "subject_cov": 0.8904593639575972, "identity": 1.0, "score": 503.0, "evalue": 1.17e-179, "valid": true, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "IGIGPGHMMC_1125", "locus": "GCA000148625_01113" }, { "type": "cds", "sequence": "contig_1", "start": 631150, "stop": 631428, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTTTAGCTGAAGGAGATAAAATCGTTTATTATCCAAATGAAGCTGAAGCTCAAGATGCTAAAAAAGAAGCTAAAGTTAAAGCTAAAAAAGCTGAAGATAAAAAACAAGCAGCAGCTAAAGAAGCAGAAATTGCTAAAAAACTTGCTGCTAAAAAAGCTAAAGCAGCTGAAAGCAAAAAAGCAGACAGCTCAAAAGCTGAAAAAACTACAAAATCAACTACTTCAAAACCTGCTGCTAAAAAAGTTGCAGCTGCTAAGAAGAGTTCAAAATAA", "aa": "MITLAEGDKIVYYPNEAEAQDAKKEAKVKAKKAEDKKQAAAKEAEIAKKLAAKKAKAAESKKADSSKAEKTTKSTTSKPAAKKVAAAKKSSK", "aa_hexdigest": "792aa382020172fe834ae68c3dcba999", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9736.16699999999, "isoelectric_point": 9.875154304504395 }, "id": "IGIGPGHMMC_1126", "locus": "GCA000148625_01114" }, { "type": "cds", "sequence": "contig_1", "start": 631722, "stop": 631895, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAATCTCAACTAAAGATGCTGTTAACAAACTTAAATCATTAAAAATTGAAAACTTAAAACACATTTTAGTGGTAACAACAGATGAAACTGTTTACAAATCAACAGCTAACTTACAAAATGTTTTATGTGTTAAACCAAACTCAGTAAGTGTTGAAAACCTTGTTTGA", "aa": "MSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLV", "aa_hexdigest": "f17e344e8b3a504831f257ea37f66ff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6299.3160000000025, "isoelectric_point": 9.25226078033447 }, "id": "IGIGPGHMMC_1127", "locus": "GCA000148625_01115" }, { "type": "cds", "sequence": "contig_1", "start": 632091, "stop": 632795, "strand": "-", "frame": 3, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_C4XG14", "UniRef:UniRef90_C4XG14" ], "nt": "ATGGCTGAAACAAAGAAAAAAGCTACTACTAAAGCTAGTGAAGAAAAGAAAACTATAACTAAAAAAGTAGCAACTGCTAAGAAAACTACTACTGCTAAATCAACTTCTACAGCTAAAAAATCAACAACTACTGCTAAAAAAGCTACTACAACAACTAAAAAATCCGCTGCTCCTAAAGCAACAGCTGAAAAGAAAACAACTTCAAGAAAAAAAGTTACAGTTGAAATTGATTTAACTAAAAACTTAAAACCTAAAAAACCTACTAAAGCTGAATTAAAAGCTCAAGAAGCTAAAGAAGAAAGTGCTAAAAAGAAAACCGCTGCTAAAACAGCTGCTCAAAAAGCTAAATTAGCTAAAGAAGAAGCTGCTGCAAAAGCAAAAGCAGAAAAAGAAGCACAAGCAGCTTTAGATGAATTAAAAGCTGCAGCAGAAGCTGCTAAAAGAAAAGCTAAAACTTATGCATCTTCAAGAGCAAAAGTTGCTTCACTTAAATTAAATGATGCTAAACTTGAACAAGAAAAATTTACAAAAGTTATCTTGCTTCACTTTGACAATAAAGCCTTACCAAAAGAATTATTTGCATCACCAAAAATTTATGAACAAGCAATTTTTGACACCATTATGGCTGATAGAGCATCAAGACGTCAAGGTACTCATGATGTTAAAAATCGTTCAGAAGTAAGTGGTGGTGGTAAAAAACCTTGA", "aa": "MAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKP", "aa_hexdigest": "9ae2affe01022b21dd6220ad6a3783ba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 631788, "stop": 632798, "observations": { "mutation": [ 632039, 632093 ], "stop": [ 632039, 632093 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG14", "query_cov": 1.0, "subject_cov": 0.6190476190476191, "identity": 1.0, "score": 215.0, "evalue": 4.45e-65, "gene_start": 1, "gene_end": 234, "reference_start": 2, "reference_end": 235, "reference_sequence": "MMAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKPWRQKGTGRARAGSTRSPIWVGGGRAFGPTPERNYSLKVNKKVRRAAFLSALTLLSNKKSVVIDDFKMSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLVWADVLVLSNEGLKNFEGRIK" }, "paralog": false, "identity": 0.9880952380952381, "score": 410.0, "evalue": 3.5e-144, "description": "Internal stop codon at 632039, 632093. Nonsense mutation around 632039, 632093.", "psc": { "uniref90_id": "UniRef90_C4XG14", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_C4XG14", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG14", "UniRef:UniRef50_C4XG14", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG14" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "IGIGPGHMMC_1128", "locus": "GCA000148625_01116" }, { "type": "cds", "sequence": "contig_1", "start": 632795, "stop": 633142, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGCCAGGTCACTTAGGAGCTGTTAGAACAACAGTTCAAAATCTTGAAATTGTTAAAGTTGATGTAGCAAACAACATTATGTTAGTTAAAGGATCAATTCCTGGGGCTAAAAATTCATATGTTATGATTCGTGAAGCTGTTAAAGGCTTACCAAATCATGATGCAATTAAACTTGTTGATATTAAAGAAGTTCTTAAAATGAATGAATTGCTTGAAAGAGCTAAAAAATACAACATTGAAGTTACAGTTGGTATGCATTCAAGTGAATTAGAACCTCTTATCCAAAAAGCTGAAGCTGATGCAGCTGCAGCAGCTGAAGAAAGTAAAAAAGCAGAAGGAGATAAATAA", "aa": "MPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK", "aa_hexdigest": "acab8c86c449961495c19a7a3340cc7a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 632795, "stop": 633442, "observations": { "mutation": [ 633256 ], "stop": [ 633256 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4166666666666667, "identity": 1.0, "score": 212.0, "evalue": 2.72e-67, "gene_start": 1, "gene_end": 115, "reference_start": 162, "reference_end": 276, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9953488372093023, "score": 420.0, "evalue": 1.9e-151, "description": "Internal stop codon at 633256. Nonsense mutation around 633256.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "IGIGPGHMMC_1129", "locus": "GCA000148625_01117" }, { "type": "cds", "sequence": "contig_1", "start": 633254, "stop": 633622, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGAAAGGAATCTTAGGACGGAAGATTGGTATGACACAAATCTTTACTGAACTTGGAGCTAGTGTTCCAGTTACAGTTATAGAAGTGCAACCAAATGTTGTTACTAAAGTTCTTTCAGCTGACAAAAATGGGTATGTTGCAACCCAAATTGCTGTTGGTGAAAAGAAAGAACACAGAAGCAATAAACCTCTTAAAGGTCAATTTGCTCAAGCTAAAACAACACCTAAGCGCTACGTTAAAGAAATTCGTGGCATGCAAGGCTATGAATTAGGTCAAACTATTAAGGCCAACATTTTTAAAGCTGGTGAATTAGTAGATATCACTGGTACATCAAAAGGTAAAGGTTTTGCCGGTACAATCAAAAGATGA", "aa": "MKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKR", "aa_hexdigest": "5cd896c66477f169406bf9b1ac3518b2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 632951, "stop": 633625, "observations": { "mutation": [ 633256 ], "stop": [ 633256 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4420289855072464, "identity": 1.0, "score": 233.0, "evalue": 2.33e-75, "gene_start": 1, "gene_end": 122, "reference_start": 2, "reference_end": 123, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9910714285714286, "score": 437.0, "evalue": 3.53e-158, "description": "Internal stop codon at 633256. Nonsense mutation around 633256.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "IGIGPGHMMC_1130", "locus": "GCA000148625_01118" }, { "type": "cds", "sequence": "contig_1", "start": 633694, "stop": 633999, "strand": "-", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354782.1", "SO:0001217", "UniParc:UPI0001A8C186", "UniRef:UniRef100_C4XG16", "UniRef:UniRef50_Q98PX9", "UniRef:UniRef90_C4XG16" ], "nt": "ATGAAAAAAATCAATATCAAGGTTAAAAGTTTTGATCATGCTTTAGTTGATTTTGGAGCAAAGAAAATTGTTGAAGTTGCTACCAAAAACTCAGTTAAAGTAAGTGGTCCAGTTCCTCTTCCAACAAAAAGAGAAGTTGTGACTATCTTAAGATCAGTTCACGTTAACAAAAAATCTCGTGAACAATTTGAATCAAGAACTTTTCAACGTTTGATCGTTTTATTAAACCCAAACGATAAAGCTCTTGACCAAATTAAGAGATTAGAACTTCCAGCAGGTGTTGAAGTTCAAATCTCAACAAAATAA", "aa": "MKKINIKVKSFDHALVDFGAKKIVEVATKNSVKVSGPVPLPTKREVVTILRSVHVNKKSREQFESRTFQRLIVLLNPNDKALDQIKRLELPAGVEVQISTK", "aa_hexdigest": "15182ac8da1878398a66916b08ae983e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C186", "ncbi_nrp_id": "WP_013354782.1", "uniref100_id": "UniRef100_C4XG16", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C186", "RefSeq:WP_013354782.1", "UniRef:UniRef100_C4XG16" ] }, "ips": { "uniref100_id": "UniRef100_C4XG16", "uniref90_id": "UniRef90_C4XG16" }, "psc": { "uniref90_id": "UniRef90_C4XG16", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_Q98PX9", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PX9" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "IGIGPGHMMC_1131", "locus": "GCA000148625_01119" }, { "type": "cds", "sequence": "contig_1", "start": 634629, "stop": 635075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTTTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACCAAGATCAAAATAACATTACAGTTAGCGCCAATGAAAAAGGTTTAGGTTACTATCCAGAAACAATACAAAGATTAATTAAAGGCAAAAAAAACAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAACGTTTATCATGATGATATACACAAATTTGATAGACTCGAGAAAGTTTACTTTTGA", "aa": "MKKKLFFNFSLLSSVALPLTTIAASCNQNQDQNNITVSANEKGLGYYPETIQRLIKGKKNKELIDTINQFINNKEQIKYENNKEYSLNENESTFGYNELKKEYKCIIALNIKDNDKTKVKKFSINKIGHNNVYHDDIHKFDRLEKVYF", "aa_hexdigest": "3ac84ba6cda4564a63f6a310c60fa181", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17335.520699999997, "isoelectric_point": 9.164003562927245 }, "id": "IGIGPGHMMC_1132", "locus": "GCA000148625_01120" }, { "type": "cds", "sequence": "contig_1", "start": 635541, "stop": 635768, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGA", "aa": "MTSKDFLDLLDKVDKEHNHEPKDIINFLKQYVNIDGTYDANDKYEYAFNVEKTHTHGSGQLHLYTYYREKGKNQK", "aa_hexdigest": "137ca94b2b95e6cad4d06177a5b7958a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.7844, "isoelectric_point": 6.165973472595215 }, "id": "IGIGPGHMMC_1133", "locus": "GCA000148625_01121" }, { "type": "cds", "sequence": "contig_1", "start": 635814, "stop": 636029, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGA", "aa": "MGGLKFNNAGIKDAKQANEDPGFYTSPHQILREMETKSTFDEQLAVLKKYCEVKDESDKNKYDYKFNFKES", "aa_hexdigest": "49406b5a7f89a963e2a6e0704ca5ebec", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8260.130999999998, "isoelectric_point": 5.769635200500488 }, "id": "IGIGPGHMMC_1134", "locus": "GCA000148625_01122" }, { "type": "cds", "sequence": "contig_1", "start": 636852, "stop": 636983, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGA", "aa": "MRLDLKNFGAFSKVGDFLIKNTPSETDIVNVRAQDIINKAKSI", "aa_hexdigest": "4722c44c811fc246110dd46ef1c40979", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4808.513800000001, "isoelectric_point": 9.397443580627442 }, "id": "IGIGPGHMMC_1135", "locus": "GCA000148625_01123" }, { "type": "cds", "sequence": "contig_1", "start": 637059, "stop": 637232, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTGAAGAACACTAAAACAAATGAAGAAAAAAATGTTTATATTCTATTTTGCAAATTAGCCTAA", "aa": "MGFPGYDNTVINEDLEYTIDFNKAVVYKSGKLELNIKVKNTKTNEEKNVYILFCKLA", "aa_hexdigest": "d7b1e956af15537372c07a99b7f7efe0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6577.4884, "isoelectric_point": 6.1446020126342775 }, "id": "IGIGPGHMMC_1136", "locus": "GCA000148625_01124" }, { "type": "cds", "sequence": "contig_1", "start": 637974, "stop": 638861, "strand": "+", "frame": 3, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_Q07809", "UniRef:UniRef90_C4XG20" ], "nt": "ATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCACTTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGA", "aa": "MKSKNIEIKNKDKELKEALEEITKKFGSEAIMLFGQNEIEEKIETFPSGSYLLDNALGIGGYPKGRIIEIYGPESSGKTTLCLHAIAEIQKQGGIAAYIDAEHSLDPNYARNLGINLDNLILSQPDSGEQALQIVDILAKSGSIDLIVVDSVAALVPEVELNGTMSDSSVGALARLMAKGLRKITGNLNKNKTAIIFVNQIREKIGVLFGNPETTTGGRALKFFSSVRIEVRKTTSIVEGKDITGNEVKIKIVKNKLAAPYKSFTTEIIFSKGIDKLNELINLATEANVIEKKGA", "aa_hexdigest": "d8c954a88b72b750d6748c191b49959b", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XG20", "query_cov": 1.0, "subject_cov": 0.8651026392961877, "identity": 0.997, "score": 557.0, "evalue": 1.81e-199, "valid": true, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_Q07809", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_Q07809", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q07809" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "IGIGPGHMMC_1137", "locus": "GCA000148625_01125" }, { "type": "cds", "sequence": "contig_1", "start": 639216, "stop": 639383, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_C4XG21", "UniRef:UniRef90_UPI000FF82D94" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTCCAAATAGAAGATGTTAGAAACAATTATCAAAACTTTTTAGGCTTGTTTAGCGAAGTAAGACTAAGAGTTAGATTATGCAGAAAAGAAAACATGCATTTTAGTGATGAATTACAAATAGATGCTTGTATCCATGAATGA", "aa": "MIKIAELLKVQIEDVRNNYQNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHE", "aa_hexdigest": "f8f53ede93574aece135216a50673eb5", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.909, "score": 103.0, "evalue": 3.08e-28, "valid": true, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 639117, "stop": 639383, "observations": { "mutation": [ 639131, 639218 ], "stop": [ 639131, 639218 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG21", "query_cov": 1.0, "subject_cov": 0.625, "identity": 0.982, "score": 112.0, "evalue": 1.76e-31, "gene_start": 1, "gene_end": 55, "reference_start": 1, "reference_end": 55, "reference_sequence": "MIKIAELLKVQIEDVRNNYKNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHEWFDGTKNAYYHAIDVAIIIIYFFAFVFYIWFRY" }, "paralog": false, "identity": 0.9659090909090909, "score": 172.0, "evalue": 1.71e-57, "description": "Internal stop codon at 639131, 639218. Nonsense mutation around 639131, 639218.", "psc": { "uniref90_id": "UniRef90_C4XG21", "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG21", "UniRef:UniRef50_C4XG21" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6619.632799999998, "isoelectric_point": 6.0718481063842775 }, "id": "IGIGPGHMMC_1138", "locus": "GCA000148625_01126" }, { "type": "cds", "sequence": "contig_1", "start": 639943, "stop": 640173, "strand": "+", "frame": 1, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGA", "aa": "MTKENKTLNILFIGDIFSEAGIEIVEKHLPSLIKENKIDVVIAQSENVSGRKGLVKKDYLRLKKAGVNIFTIGNHV", "aa_hexdigest": "2a3296f3a3bd25315eab08a421cf3e34", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9078947368421053, "subject_cov": 1.0, "identity": 0.58, "score": 89.7, "evalue": 3.53e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "IGIGPGHMMC_1139", "locus": "GCA000148625_01127" }, { "type": "cds", "sequence": "contig_1", "start": 640516, "stop": 640761, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "GTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAA", "aa": "MQTNDAHVLPKGTCYLTDAGMSGPFDCAIGANFDEVYQKMRYNKNVKFIPSKNKSQFNGAILKLNSDKSQNKIIPINIRQE", "aa_hexdigest": "9d9c00019db0b50b5a1ddd9eecb21784", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 73, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.2225187224620877e-18, "score": 63.27640914916992, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 9110.331299999996, "isoelectric_point": 9.230534934997557 }, "id": "IGIGPGHMMC_1140", "locus": "GCA000148625_01128" }, { "type": "cds", "sequence": "contig_1", "start": 640920, "stop": 641105, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24", "UniRef:UniRef50_C4XG24", "UniRef:UniRef90_C4XG24" ], "nt": "GTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAA", "aa": "MILSGILYRYFFTIYYKICKDKKEIKIMKYPKSFVDFSNIDVDLEASNELFLMGPLVFLFY", "aa_hexdigest": "d7bfa93e70bb7dd942a257ab1b248e14", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C18E", "uniref100_id": "UniRef100_C4XG24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24" ] }, "ips": { "uniref100_id": "UniRef100_C4XG24", "uniref90_id": "UniRef90_C4XG24" }, "psc": { "uniref90_id": "UniRef90_C4XG24", "uniref50_id": "UniRef50_C4XG24" }, "pscc": { "uniref50_id": "UniRef50_C4XG24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG24" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7363.714, "isoelectric_point": 7.796629142761231 }, "id": "IGIGPGHMMC_1141", "locus": "GCA000148625_01129" }, { "type": "cds", "sequence": "contig_1", "start": 641676, "stop": 642266, "strand": "-", "frame": 3, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGTCAGGTGAAGCAATTGATATGCCTAATAATTTAATTGCTGGTAAAGCAATGGATAATAGAGCTGGTGTTACAGTTATTGACTTTTTAGCAAACAATATTAAAGATTTAGATTTACCAAATGAAACATATATTGTTGGTACAGTACAAGAAGAAATTGGAACAAGAGGTGCTAAAACTTCAGTTTCAATTATTGAACCAGATGTTGCTTTTGCAATTGATACAGGAGCAAGTCATGATACGACAGGATGTATTAAAGGAACTCCAGTTTTAGGTAAAGGTGTAGCTCTTTTAATACAAGATGGTGCTGTTATTACAGATCCTAAATTAGTTAACATGTTAGTAAAAATTGCTGAAAAACACAAAATTCCAGCTTATAAATATATAGCCGAAGGTGGCGGTACTGATGGAGCTAGTTTACAATATGGTAAAGGTGGGGTTCCTACAATTACTCTAAGTTTACCTCAACGTTATTTACACTCACCAATAGGCGTTTGTTCATTAGTAGATATTCAAGCAACAATTGATTTAATTACAGAATTTGTCAAAGCTTTTGACAACAAAATGTTAAATGAATTAAAAGCTAAATAA", "aa": "MSGEAIDMPNNLIAGKAMDNRAGVTVIDFLANNIKDLDLPNETYIVGTVQEEIGTRGAKTSVSIIEPDVAFAIDTGASHDTTGCIKGTPVLGKGVALLIQDGAVITDPKLVNMLVKIAEKHKIPAYKYIAEGGGTDGASLQYGKGGVPTITLSLPQRYLHSPIGVCSLVDIQATIDLITEFVKAFDNKMLNELKAK", "aa_hexdigest": "aa14b09513a8db6338a005c2107b1327", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 1.0051020408163265, "subject_cov": 0.933649289099526, "identity": 0.5529999999999999, "score": 225.0, "evalue": 5.93e-72, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "IGIGPGHMMC_1142", "locus": "GCA000148625_01130" }, { "type": "cds", "sequence": "contig_1", "start": 642294, "stop": 642446, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAACAAATAGAGACAATAAAGAAATTTTTGGTATTTTTGGTCATACATCAATTCATATTATGGAAGCTGAAAAACGTGCTAAAGTTCCTACAACAAAAGAAATGTTTGTTGACTGCGGATTCAATTCAAAAGAAGAAGCTGAAAAATGA", "aa": "MVTNRDNKEIFGIFGHTSIHIMEAEKRAKVPTTKEMFVDCGFNSKEEAEK", "aa_hexdigest": "f7e0d5ae347e5fe47023b9314bbd5913", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5745.502199999999, "isoelectric_point": 6.056331062316894 }, "id": "IGIGPGHMMC_1143", "locus": "GCA000148625_01131" }, { "type": "cds", "sequence": "contig_1", "start": 642477, "stop": 642761, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATATGATAAATTAGCAAAAAGATTAAAAGAATACATGGCTATTGAAGCTATGAGTAGATATGAAGAACCTGTTGTTGAAGCTTTAAAGAAGAATACAGCTTCAAAAAATTTTGAATATTCAAGAGATGGTTTTGGTTCTTTAATTATTAAAAATAAAACAAAAGTTAAAAATGCACCAAAAATTATGATTGCTGCACACATGGATGAAGTTGGTTATTTAGTTAGATCAATCGAACCAAATGGTAACTTATTAGTTTCAGTTGTTGGAGGTGTTTGA", "aa": "MAKYDKLAKRLKEYMAIEAMSRYEEPVVEALKKNTASKNFEYSRDGFGSLIIKNKTKVKNAPKIMIAAHMDEVGYLVRSIEPNGNLLVSVVGGV", "aa_hexdigest": "d833bab3ea9363b9634ee1fce8aed2cc", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10508.202299999999, "isoelectric_point": 9.486925697326662 }, "id": "IGIGPGHMMC_1144", "locus": "GCA000148625_01132" }, { "type": "cds", "sequence": "contig_1", "start": 643547, "stop": 643765, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGA", "aa": "MKKTVKNKKNNEALIIKNRLVEAANMNQNEIYEKYNSSNLGITKNEIIEENKENYGVNKLSKKKRGGVLKRI", "aa_hexdigest": "f4d0319613161825ce66d2a0340f6dd1", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 48, "aa_cov": 0.6527777777777778, "hmm_cov": 0.9791666666666666, "evalue": 6.578755263264515e-07, "score": 24.98471450805664, "start": 25, "stop": 71 } ], "seq_stats": { "molecular_weight": 8336.564999999995, "isoelectric_point": 9.946262931823732 }, "id": "IGIGPGHMMC_1145", "locus": "GCA000148625_01133" }, { "type": "cds", "sequence": "contig_1", "start": 643708, "stop": 643884, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACAGTTCAAGGTTCGGATTTTTCACCTTTAGTTAAAGGAAGTATTATGTCAGTAATTAATGAAACTATTGAAAGTAAAATCAAGATCAATGAAAAAGGATTGAAGAAGCTATCTCATATTCTTTTTAATACTCCGCCTCTTTTCTTTTTGCTGAGTTTATTAACTCCATAA", "aa": "MDTVQGSDFSPLVKGSIMSVINETIESKIKINEKGLKKLSHILFNTPPLFFLLSLLTP", "aa_hexdigest": "903eb4c9a151afbd9cd8e2011158b000", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6431.557799999997, "isoelectric_point": 8.121356391906737 }, "id": "IGIGPGHMMC_1146", "locus": "GCA000148625_01134" }, { "type": "cds", "sequence": "contig_1", "start": 643895, "stop": 644368, "strand": "+", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGA", "aa": "MVIISGILHFVEETRSSASAEKLVKMIQTTTRVERNGVAYEIPLEEVVVGDIVVLAAGDIIPADVRILSATDLFVSQSQLTGESDSIEKFPNLVPNYDYDNVTDYHNLAFMGSNVISGSAKAMVVVTGDQTYIGQVAQKINEKAPKTDFEKGIKSIS", "aa_hexdigest": "fb3ae051800ebd2e30134e9a80545abf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 1.0063694267515924, "subject_cov": 0.8272251308900523, "identity": 0.551, "score": 156.0, "evalue": 1.46e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "IGIGPGHMMC_1147", "locus": "GCA000148625_01135" }, { "type": "cds", "sequence": "contig_1", "start": 644384, "stop": 644710, "strand": "+", "frame": 2, "gene": null, "product": "Magnesium-transporting ATPase, P-type 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9TWP6" ], "nt": "ATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGCGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAA", "aa": "MLVAVPLVLIITGTRASIKGEHEKWVDALLFAISVAIGLTPEMLPMIITSTLAKGAMSMSKKQTIVKSLNSIQNFGAMDIFCTDKTGTLTLDQSCARKTLGCKRLRKY", "aa_hexdigest": "5f8a8fb41946b59310969966a97cc0c0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9TWP6", "query_cov": 0.8796296296296297, "subject_cov": 0.8636363636363636, "identity": 0.642, "score": 115.0, "evalue": 1.75e-31, "valid": false, "product": "Magnesium-transporting ATPase, P-type 1", "uniref50_id": "UniRef50_A0A4U9TWP6" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9TWP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9TWP6" ], "product": "Magnesium-transporting ATPase, P-type 1" }, "genes": [], "id": "IGIGPGHMMC_1148", "locus": "GCA000148625_01136" }, { "type": "cds", "sequence": "contig_1", "start": 644729, "stop": 645772, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32", "PFAM:PF08282.18" ], "nt": "TTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAGTGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGCAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTACGATTTTGCTTGTGGAACAATTCCTTGA", "aa": "MNSYYQTGLKNLLDISIINKTDELSDVEMELRSLEEIYKKVDEIPFDFIRKRMSVVVKSIKSDKTQLITKGAVEEILSICSKLEYKGEVVDLDEKMIQKVLKQVDKLNDEGMRVIAVARKSNPSQVGKFGVADEKDMILIGYLAFLDPPKESTKSAVENLHNLGVEVKILTGDNARVTRAICAQVGIPTDKIMLGKDLMELSDEELQKVANEYNIYAKLSPDQKARVITALRANGHVVGYMGDGINDAPAMKVADVSISVDTAVDIAKESANIILLEKDLNVLATGITEGRKTYANMNKYVKMTVSSNFGNIISMILASILIPFVPLMAIQVLFLNLIYDFACGTIP", "aa_hexdigest": "cd96e00d188592733ae9ce09bad5ff08", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003261AFD9", "query_cov": 1.0, "subject_cov": 0.8183962264150944, "identity": 0.51, "score": 337.0, "evalue": 1.27e-110, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_A0A2X2TA75" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 136, "aa_cov": 0.36023054755043227, "hmm_cov": 0.8235294117647058, "evalue": 3.0311183080714817e-15, "score": 53.109718322753906, "start": 131, "stop": 255 }, { "id": "PF08282.18", "name": "Hydrolase_3", "length": 45, "aa_cov": 0.12968299711815562, "hmm_cov": 1.0, "evalue": 3.053455937423978e-06, "score": 23.115222930908203, "start": 237, "stop": 281 } ], "seq_stats": { "molecular_weight": 38373.294100000014, "isoelectric_point": 5.282013511657714 }, "id": "IGIGPGHMMC_1149", "locus": "GCA000148625_01137" }, { "type": "cds", "sequence": "contig_1", "start": 646763, "stop": 646933, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGTAGCACTTGATCCTAAATATATTGACTATGTGATAGTTCATGAATTATGCCACTACTTTCATATGGATCATTCTGAAGAATTTTATAAATTGGGTGAATTTTATTATCCTAATTTTAAAGAATATTCAGACACAATTAATAAACATAAAATTAATTTTTGA", "aa": "MFLVALDPKYIDYVIVHELCHYFHMDHSEEFYKLGEFYYPNFKEYSDTINKHKINF", "aa_hexdigest": "5faaf5c4b99da39c58ab7e5bf33bc1fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6983.862699999998, "isoelectric_point": 5.582237052917479 }, "id": "IGIGPGHMMC_1150", "locus": "GCA000148625_01138" }, { "type": "cds", "sequence": "contig_1", "start": 647213, "stop": 647530, "strand": "-", "frame": 1, "gene": null, "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30", "UniRef:UniRef90_C4XG30" ], "nt": "ATGACTCAAAGAAAAAATAATTTAGTTTTAATTAAACACATCATAATTGATGATGTTAGTTATCCAGTTTATTCAAAAGTTAATGTCCGTTGTGAAAATATAATCGTTAGATTTAATAGTGACCAAAAAGTATTTGAGGTCACTAGTTTTAGACATATTAGTGAGGCTGATTATAATTTTTATAATTTAGATAGACTACTTATTAAAGCTTTTAAAAAAGTAAATAAAAGATTAAATAATGCTATAGTTTTAAATTTAACTAGCGAAGTTGAAGAACACTACATTAGTTTTTTTGGCAAAGAAACATTATTAAAATGA", "aa": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFYNLDRLLIKAFKKVNKRLNNAIVLNLTSEVEEHYISFFGKETLLK", "aa_hexdigest": "fd5bb6ccc6c1c12c9993f5502f3c5d18", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 646910, "stop": 647530, "observations": { "mutation": [ 646984, 647215 ], "stop": [ 646984, 647215 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG30", "query_cov": 1.0, "subject_cov": 0.41015625, "identity": 0.971, "score": 201.0, "evalue": 2.45e-63, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFHNLDRLLIKAFKKANKRLNNAIVLNLTSGVEEHYISFFGKETLLKWSFHYRDTSDGVYDKENNILTIRCSIKCASDQNKRLKVFEKTLTKFLEDYISKKQLVYSALMQINNPYFYVRMKKSAWGTHHKKYNVFSKLSYSLFLVALDPKYIDYVIVHELCHYFHMDHSEEFYKLGEFYYPNFKEYSDTINKHKINFW" }, "paralog": false, "identity": 0.9660194174757282, "score": 395.0, "evalue": 3.47e-142, "description": "Internal stop codon at 646984, 647215. Nonsense mutation around 646984, 647215.", "psc": { "uniref90_id": "UniRef90_C4XG30", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG30", "UniRef:UniRef50_C4XG30" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30" ], "product": "YgjP-like metallopeptidase domain-containing protein" } }, "id": "IGIGPGHMMC_1151", "locus": "GCA000148625_01139" }, { "type": "cds", "sequence": "contig_1", "start": 647511, "stop": 647648, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAGATCGAAACTTAAATAAAGATGAATTAATGTTAATCAATGAATCAGTGAGTTCAAATAGAAGTGCTTCACTTAAAGACATTAAACGTTTTGGAAAGATCTTAAACAAAAATGACTCAAAGAAAAAATAA", "aa": "MSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK", "aa_hexdigest": "8e005d109165793d4f611c438c2825e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5195.8859, "isoelectric_point": 9.77445468902588 }, "id": "IGIGPGHMMC_1152", "locus": "GCA000148625_01140" }, { "type": "cds", "sequence": "contig_1", "start": 647706, "stop": 648212, "strand": "-", "frame": 3, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31", "UniRef:UniRef90_C4XG31" ], "nt": "ATGATTAAACCAAGTAGAGAAATGATAATTGGACTATCAGTTGGAATTCCTTTAACACTATTAATTATTGTGCTAGTGTTAACTTTTTTAATTATCAAACTTATTAAAAGCAAACGCAATAATTCAATTGGTTTTCGTTTTGAAGCAAAAATTAGAAATAAACTTCTAACTATTGCAAAAGATAAAAACTACAAATACTTAAATGGTGGTTTATTTAAATATGGACAAAGTCAAATATTTGAACTTGATGGTATTTTAATTAGTAATAAGGCAGTTTTTATTATTGAAGTTAAATATTATGTTGGCCATCTTAGTGGTGAAGCTACTAATGCTGAATTAGAGTTAAGAAGCAAAAGCAATAAAGTTTTGAAATTTAAAAATCCGCTTATTCAAAACTTTAAACACATTCAACACTTTTACAAAATGTGTGGCTTTAACTTCCCAGTATTTTCATTGGTAATCTTGCCTTCAAATACTACTTTTGATATAGCTAACTTAGAATCATGA", "aa": "MIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLES", "aa_hexdigest": "b7fec0ca1287f650dd67e23f100062f5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 647511, "stop": 648245, "observations": { "mutation": [ 647708 ], "stop": [ 647708 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG31", "query_cov": 1.0, "subject_cov": 0.6885245901639344, "identity": 1.0, "score": 319.0, "evalue": 4.55e-109, "gene_start": 1, "gene_end": 168, "reference_start": 12, "reference_end": 179, "reference_sequence": "MQILILNIIINMIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLESWALVTTEDQVANLLEDVAKDMSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK" }, "paralog": false, "identity": 0.9918032786885246, "score": 465.0, "evalue": 5.01e-169, "description": "Internal stop codon at 647708. Nonsense mutation around 647708.", "psc": { "uniref90_id": "UniRef90_C4XG31", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG31", "UniRef:UniRef50_C4XG31" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31" ], "product": "NERD domain-containing protein" } }, "id": "IGIGPGHMMC_1153", "locus": "GCA000148625_01141" }, { "type": "cds", "sequence": "contig_1", "start": 648291, "stop": 648479, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAGCAGCTTTTTGA", "aa": "MSAKKIISILGSVNKNSLSNKINNIVSSKLFKKYPNSEITLLDLANSPFSELMLNGHDTAAF", "aa_hexdigest": "cce660ca8e07b256b0adeea686c1c53c", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6768.7647, "isoelectric_point": 9.523801612854005 }, "id": "IGIGPGHMMC_1154", "locus": "GCA000148625_01142" }, { "type": "cds", "sequence": "contig_1", "start": 648549, "stop": 648713, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGA", "aa": "MINFNYSALVKNFIDAICIADHSFSYKYSKKNGSVGLLNKMKVIIIGTQGAPKG", "aa_hexdigest": "261b72bc83ebc312a1971da398a55dd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9622641509433962, "evalue": 4.2416855964483955e-10, "score": 35.646446228027344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5909.8963, "isoelectric_point": 9.66846866607666 }, "id": "IGIGPGHMMC_1155", "locus": "GCA000148625_01143" }, { "type": "cds", "sequence": "contig_1", "start": 648738, "stop": 648881, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAAGGAACATTTAACTTTTTAGGGGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATACTTTAATTTAA", "aa": "MLEGTFNFLGAKEVKTLLVAGTKVTPLKDMSQEEIIKQYQKEIDTLI", "aa_hexdigest": "09287dfa1b20796dfe18fba5f91237cb", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5313.187599999998, "isoelectric_point": 5.144690513610839 }, "id": "IGIGPGHMMC_1156", "locus": "GCA000148625_01144" }, { "type": "cds", "sequence": "contig_1", "start": 648959, "stop": 649117, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "ATGTCAATTGAACCTAAAATAATAATTAATGAATCAGTTGAAATAACTAAATATTATTTTGAAGATGAAAAAAACTTTTACAAAATGGTTAATGCAATTCTAGAAAAAGTTTATAAATTCTTTGTTATCAATGAAGTTATTTTGAAAAAACATGATTAA", "aa": "MSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKHD", "aa_hexdigest": "02b423b70d01cb62163cf462734f20ef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 38, "aa_cov": 0.7307692307692307, "hmm_cov": 0.9473684210526315, "evalue": 1.639137329278804e-07, "score": 27.77552604675293, "start": 2, "stop": 39 } ], "seq_stats": { "molecular_weight": 6345.378800000001, "isoelectric_point": 5.755027580261231 }, "id": "IGIGPGHMMC_1157", "locus": "GCA000148625_01145" }, { "type": "cds", "sequence": "contig_1", "start": 649178, "stop": 649378, "strand": "-", "frame": 1, "gene": null, "product": "NusB/RsmB/TIM44 domain-containing protein", "db_xrefs": [ "KEGG:K03625", "SO:0001217", "UniRef:UniRef50_C4XG33", "UniRef:UniRef90_C4XG33" ], "nt": "ATGCAAAACAGACGTCAATTTCGTTTAGGTATAATTAATGTTCTTTACCGTTATGAATTAATGGATCAAAAAATCAATGTCAATGAATTATTTGATTCAGAAAGTAACTTAAGTAATGAACAATTTTCGCATCTTGAAAAGATAGCAAAAAACTATGATTTTTATAAAAAAGCAGTTAGTCAATTTTTAAAAGAAGGTTGA", "aa": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEG", "aa_hexdigest": "7d736dd278c923cfe77172f929eee637", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 648959, "stop": 649378, "observations": { "mutation": [ 649174, 649180 ], "stop": [ 649174, 649180 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG33", "query_cov": 1.0, "subject_cov": 0.4748201438848921, "identity": 1.0, "score": 129.0, "evalue": 2.5e-37, "gene_start": 1, "gene_end": 66, "reference_start": 1, "reference_end": 66, "reference_sequence": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEGWSWERISPLIRAILINGAHELMSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKQD" }, "paralog": false, "identity": 0.9712230215827338, "score": 263.0, "evalue": 1.41e-92, "description": "Internal stop codon at 649174, 649180. Nonsense mutation around 649174, 649180.", "psc": { "uniref90_id": "UniRef90_C4XG33", "product": "NusB/RsmB/TIM44 domain-containing protein", "uniref50_id": "UniRef50_C4XG33", "kegg_orthology_id": "K03625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG33", "UniRef:UniRef50_C4XG33", "KEGG:K03625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG33" ], "product": "NusB/RsmB/TIM44 domain-containing protein" } }, "id": "IGIGPGHMMC_1158", "locus": "GCA000148625_01146" }, { "type": "cds", "sequence": "contig_1", "start": 649452, "stop": 649862, "strand": "-", "frame": 3, "gene": null, "product": "DUF1934 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354794.1", "SO:0001217", "UniParc:UPI0001A8C198", "UniRef:UniRef100_C4XG34", "UniRef:UniRef50_C4XG34", "UniRef:UniRef90_C4XG34" ], "nt": "ATGAAAATAAGATTTACTTCAATTCAAGATCATAAAATTAAAAACCAAGAACCTAAAATAGTTTCTTTTGAAGCTGATGCAGAATATGATTTATTTTTAGATGATGAAGATCCAAAGCAATTACAATATCATACATATCATTTTAAAGAGCCTTCAATTGGTGAATCTAACCGTATAGAAATCAATAGTGAAAGAGTAAATATTTTTACAGGAATGTCAACGTTGACAATGTTAAAAGGTCAAAAAGCTCCTAACTCTTTTGTTAAAGTAGATGGCACACAATTCTTTTTAAAACCATTTTTACATGAAGTTGTTATTGAAGGCAATATTAAAAAATTCAAATATGAATTATTTGGCCCTAAAGATGATTTAATTGGTGAATTCAATGTTTCGATTGAAGAATTAGAATAA", "aa": "MKIRFTSIQDHKIKNQEPKIVSFEADAEYDLFLDDEDPKQLQYHTYHFKEPSIGESNRIEINSERVNIFTGMSTLTMLKGQKAPNSFVKVDGTQFFLKPFLHEVVIEGNIKKFKYELFGPKDDLIGEFNVSIEELE", "aa_hexdigest": "2174371c5bb31c858753072833bd3158", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C198", "ncbi_nrp_id": "WP_013354794.1", "uniref100_id": "UniRef100_C4XG34", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C198", "RefSeq:WP_013354794.1", "UniRef:UniRef100_C4XG34" ] }, "ips": { "uniref100_id": "UniRef100_C4XG34", "uniref90_id": "UniRef90_C4XG34" }, "psc": { "uniref90_id": "UniRef90_C4XG34", "product": "DUF1934 domain-containing protein", "uniref50_id": "UniRef50_C4XG34" }, "pscc": { "uniref50_id": "UniRef50_C4XG34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG34" ], "product": "DUF1934 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1159", "locus": "GCA000148625_01147" }, { "type": "cds", "sequence": "contig_1", "start": 649859, "stop": 650137, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "ATGCAGCCATATTTAAAACCAACAAATAAAAAACCAGCTGTTGAAGCTGAAAATCATGATGTAATTTTAACTTTTAAAGATTTATTAAATAAAAACACTTTTACTATTGAAAATATTCAAGCTGCAATTGACCAAACAAAAGAAATTACAGGTAAAAAAGGCAAAAATCTATTTATGCCAATACGTCTAGCAACCACTTACATTGAACATGGTCCTGAATTAGCTAAAGCAATTTATTTATATGGCGAAGACTTAATTAAAGAAAGGTTAGCAAAATGA", "aa": "MQPYLKPTNKKPAVEAENHDVILTFKDLLNKNTFTIENIQAAIDQTKEITGKKGKNLFMPIRLATTYIEHGPELAKAIYLYGEDLIKERLAK", "aa_hexdigest": "bf0801566ddb37e91d32329171081598", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 81, "aa_cov": 0.8695652173913043, "hmm_cov": 1.0, "evalue": 3.54929673791717e-10, "score": 36.443641662597656, "start": 13, "stop": 92 } ], "seq_stats": { "molecular_weight": 10501.095899999991, "isoelectric_point": 8.831411170959473 }, "id": "IGIGPGHMMC_1160", "locus": "GCA000148625_01148" }, { "type": "cds", "sequence": "contig_1", "start": 650387, "stop": 650503, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0", "UniRef:UniRef90_UPI0021FF3334" ], "nt": "ATGGAAGGTAAAAAACTTTCAAAACGTGATACTAGTTTAAAACAATTTATTGAAGATTATCGTAATGAAGGCTACCATCCTGAAGGTGTATTTAACTTTTTAAGTTTGCTTGGTTGA", "aa": "MEGKKLSKRDTSLKQFIEDYRNEGYHPEGVFNFLSLLG", "aa_hexdigest": "0fa7d902c7d175366f672234bafc8822", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 650339, "stop": 650503, "observations": { "mutation": [ 650389 ], "stop": [ 650389 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF3334", "query_cov": 1.0, "subject_cov": 0.6909090909090909, "identity": 0.868, "score": 73.6, "evalue": 1.28e-16, "gene_start": 1, "gene_end": 38, "reference_start": 1, "reference_end": 38, "reference_sequence": "MEGKKLSKRDTSLKQFIEDYKNEGYLPEAIFNFLTLLGWTASDASELMSKDEIIS" }, "paralog": false, "identity": 0.7407407407407407, "score": 83.2, "evalue": 2.85e-23, "description": "Internal stop codon at 650389. Nonsense mutation around 650389.", "psc": { "uniref90_id": "UniRef90_UPI0021FF3334", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF3334", "UniRef:UniRef50_A0A3B0PLI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0" ], "product": "Glutamate--tRNA ligase" } }, "id": "IGIGPGHMMC_1161", "locus": "GCA000148625_01149" }, { "type": "cds", "sequence": "contig_1", "start": 651053, "stop": 651244, "strand": "-", "frame": 1, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "nt": "ATGAATAAAATAAGAACTAGATATGCACCAAGCCCAACAGGTTATTTACACATTGGAGGTGCTAGAACAGCATTATTTTGCTATTTATATGCTAAGCACTTTAAAGGTGATTTTGTTTTCAGATTAGAAGATACTGATGTTAAAAGAAATGTAGCTGATGGTGAAAGAAGTCAATTAGAAAACTTAGCTTGA", "aa": "MNKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFKGDFVFRLEDTDVKRNVADGERSQLENLA", "aa_hexdigest": "1aa51438de5f9b6c73c89259031c2c61", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0037F98A17", "query_cov": 0.9523809523809523, "subject_cov": 0.8571428571428571, "identity": 0.5670000000000001, "score": 79.3, "evalue": 2.79e-18, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2N2SDT7" }, "pscc": { "uniref50_id": "UniRef50_A0A2N2SDT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "IGIGPGHMMC_1162", "locus": "GCA000148625_01150" }, { "type": "cds", "sequence": "contig_1", "start": 651532, "stop": 652401, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013354796.1", "SO:0001217", "UniParc:UPI0001E32EED", "UniRef:UniRef100_UPI0001E32EED", "UniRef:UniRef50_C4XG36", "UniRef:UniRef90_C4XG36" ], "nt": "ATGAAATTAAAAAAAATATTTAAATTTGGAGCCTTGTTAACTTCACCAATCATGCTAGTTTCTATTGTTTCATGTGGCAAAAATCAAAAAGATGTTAATTCAACAAAATTCTTTGAATTTGAAAGTCCGATGCCTAAAAACAATAAACCAAATAATGAAATAAGTGAAAAAGAAAAATTTCAAAATTGTCAAAACGCATATGAAAAAATTATTCAATTAGCATCATTTAAAAATGCACTTCAAAGTGATTTTGATGCAAATAACGTAAGTGAATATGGACTTGAAAAAATTTTAGATTTTAAAGCTAAAGATGGTCTTGCATTACTTAAAGCTACGTATTTTAATAAAGAATCTGAATATGAAAAAATAGTTGAAGTTTATAAATCAATTAATCCTACAGGAAACTTTAATGATTATTTAACTAATGAATATAAAAAGATGACAAGTGCAATTAATATTTATCTTAAAGCTGAAGATAATCAAAGTGCAAAAAATATTAAAGAAGAATTAAATGATCCTAAAAAAGTTAAAGAAATAAATAACAAGGTTAATAATATAAAAGAAACAATTAACAAATTGATTGATAAATTAAATGAAAATCCTGAAATCAAAAGTAAAGTTGATGAATCTTTGAAAAAACAAAACTTATCAATAGAAAAAATTAAAGAAAAAATCAGTGGTTATGATAATAATGAAATTGCAAAAGGAATTTTTCTTGGACTTAATGGAATAATAAAGAATAAAGACGAACTATTTAAAAATCCTAATTTATCAAAATTTGCTCCAATTATTCCAAACAGACCTTTTGATAATTTGAATGAATCATTAGATGTAATTAGCAATGTTTTGTCGACTGCAAATCTATTTTAA", "aa": "MKLKKIFKFGALLTSPIMLVSIVSCGKNQKDVNSTKFFEFESPMPKNNKPNNEISEKEKFQNCQNAYEKIIQLASFKNALQSDFDANNVSEYGLEKILDFKAKDGLALLKATYFNKESEYEKIVEVYKSINPTGNFNDYLTNEYKKMTSAINIYLKAEDNQSAKNIKEELNDPKKVKEINNKVNNIKETINKLIDKLNENPEIKSKVDESLKKQNLSIEKIKEKISGYDNNEIAKGIFLGLNGIIKNKDELFKNPNLSKFAPIIPNRPFDNLNESLDVISNVLSTANLF", "aa_hexdigest": "3795d214b602c66e0929ab24efa5a4e9", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E32EED", "ncbi_nrp_id": "WP_013354796.1", "uniref100_id": "UniRef100_UPI0001E32EED", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EED", "RefSeq:WP_013354796.1", "UniRef:UniRef100_UPI0001E32EED" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32EED", "uniref90_id": "UniRef90_C4XG36" }, "psc": { "uniref90_id": "UniRef90_C4XG36", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XG36" }, "pscc": { "uniref50_id": "UniRef50_C4XG36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG36" ], "product": "Lipoprotein" }, "genes": [], "id": "IGIGPGHMMC_1163", "locus": "GCA000148625_01151" }, { "type": "cds", "sequence": "contig_1", "start": 652607, "stop": 652714, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGAAATATTATTTAAAAGATGATTCTAAATTAAGTTATTTAGATTTAATTATGAATACAGCTAACAATAATGATTATTATGTAGAAATGGTTCAAGCTTGA", "aa": "MLKKYYLKDDSKLSYLDLIMNTANNNDYYVEMVQA", "aa_hexdigest": "5c74b3d0f0f832c9857cf9ed2202a669", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4208.783600000002, "isoelectric_point": 4.777908515930175 }, "id": "IGIGPGHMMC_1164", "locus": "GCA000148625_01152" }, { "type": "cds", "sequence": "contig_1", "start": 652991, "stop": 653152, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38", "UniRef:UniRef90_C4XG38" ], "nt": "ATGAATGAATTAAGATGTAGAAAAGAAGAAAAACTTTATTATAAATTAATACCTTATATTGATCAAAAATATAAAGAATTTTGCTTGCGAATTTATAAAATTGAAAGTGCAAAATTATTAGGAGTTAGAATTCCAATACTTAAAGATTTAGCTAAAAAATGA", "aa": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLRIYKIESAKLLGVRIPILKDLAKK", "aa_hexdigest": "af348264b05d0d4296eab1b75ffcb02a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 652870, "stop": 653152, "observations": { "insertion": [ 652924 ], "mutation": [ 652954, 652993 ], "stop": [ 652954, 652993 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG38", "query_cov": 1.0, "subject_cov": 0.5698924731182796, "identity": 0.981, "score": 105.0, "evalue": 1.47e-28, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLQIYKIESAKLLGVRIPILKDLAKKWYKEIPTKVSEFMWNPYHYLFEEIYFMVFLLKKLMIIKSK" }, "paralog": false, "identity": 0.956989247311828, "score": 172.0, "evalue": 1.32e-57, "description": "Internal stop codon at 652954, 652993. Frameshift due to insertion around 652924. Nonsense mutation around 652954, 652993.", "psc": { "uniref90_id": "UniRef90_C4XG38", "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG38", "UniRef:UniRef50_C4XG38" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6506.831499999999, "isoelectric_point": 9.61489543914795 }, "id": "IGIGPGHMMC_1165", "locus": "GCA000148625_01153" }, { "type": "cds", "sequence": "contig_1", "start": 653658, "stop": 653951, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTGTTATGATTGGTGGTATGGTTCCTCCTCTTGTAATCGCTATTGCAGCTGACTTATTCCCTCAAAAATTTACTGCTAAAGAAAGAAAAGATTCAAAAGTTAACTACTTAATGGGCATGGCATTTATTACTGAAGGTACAATTCCTTATGCAGCTAGTGATCCCTTGAGAGTTATTGGTTCATCAATTTTTGCAAGTACATTAGCTGGTGGAATGGCAGCTGGATTAGGATGCTCAATTCCTGCTCCTCATGGCGGAATCTTTGTATTCGTTGTTGCACAAGGATGA", "aa": "MAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGTIPYAASDPLRVIGSSIFASTLAGGMAAGLGCSIPAPHGGIFVFVVAQG", "aa_hexdigest": "385df7906a33e218c1dbc36b538bcc55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9901.656399999994, "isoelectric_point": 7.90732135772705 }, "id": "IGIGPGHMMC_1166", "locus": "GCA000148625_01154" }, { "type": "cds", "sequence": "contig_1", "start": 653955, "stop": 654221, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATCCAGTATTTGGTATCTTACTTATTGCTGTTATTATGTTCTTTGTAATTAATACACCACTTTCATATTTAAATTACTACATTCAACAAGGTTTAAACTATGTTGTTAAAAATAACACAATTTCAATTGGCTTTAAAATTGGAATGGGAGCTTTACTTGCTGGCATGATGGCAGCTGATATGGGTGGTCCAGTTAACAAAGTTGCATATACTATTGGCGTTGCTTCAGTAGATCCTGATGGAATCAATCAACCATGA", "aa": "MLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGALLAGMMAADMGGPVNKVAYTIGVASVDPDGINQP", "aa_hexdigest": "21958558f9c7950ea615262f6a325ede", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9446.161899999997, "isoelectric_point": 5.792711830139161 }, "id": "IGIGPGHMMC_1167", "locus": "GCA000148625_01155" }, { "type": "cds", "sequence": "contig_1", "start": 654264, "stop": 655511, "strand": "-", "frame": 3, "gene": null, "product": "PTS system protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I6IS36", "UniRef:UniRef90_A0AB32XC71" ], "nt": "GTGAAAATAAAAGAACTTCTAACTCTAAAAAATATTGATATTAATGCAAAAGCAAAAGATAAATTAGAAGCCATTGAATTAGCAGTTAATTTGATGGGTGATAACATTATAATTGACAAAAACGCCTATTTGACAGCGGTTTTGGAAAGAGAAAAACAAACAACAACTGGATTGGGCGATGAATTAGCATTGCCTCATGGACGCGGATCTTTCGTTAAGAAACCTCACTTAAGTGCACTTGTTTTAAAAGACGGAGTTGATTATCAATCTTTAGATAATAAACCTGTTAAATTGCTATTTCTAATTGCAGCTCCAGAAACAACAGATAGTGAAAACTTACACTTAGAAGTTTTGGCTAAATTGAGCAAAATGTTAGTTGATAAAGTATTTATTGAAAAATTAATTAATGCTAAAAGCAACAAAGAATTTCTAAAATTAATTGAAACAAAAGAACAAGAAGTTGATCAGAATGAATTTGAACAAACTAATAATGGTGAAAAATTTATAGTAGCTGTTACAGCTTGTCCAACTGGTATTGCTCATACATACATGGCTCAAGAATCTTTAGAAAATGCAGCTAAGGAAATGGGTTATCAAATCAAAGTTGAAACTCAAGGTTCAGGTGGAGCTAAAAATGTTTTGACAAATGAAGATATTCAAAAAGCTTATGGTGTCATTGTTGCTGCCGATACAAATATTGAAATCACAAGATTCAATGGTAAAAGATTGCTTCAAACAACTGTAACAAAAGGAATTAAACAACCCAAAGAATTAATACAAGAAATAATAGATGAAAAAGTAGCTGTATATAAATCAGATTCTTCTTCAAATGAAAATACAAATCAAGAAAAATTAAGTTTTGGAAAACAAATATATAAGAATTTAATGAACGGTGTAAGCCATATGCTTCCATTTGTTATTGGTGGCGGTATTCTAATTGCTCTAGCCTTCTTATTTGACTCTTTTTTAGGAAAAGAAGCAGTAGGAAAAGATTTTGGACAATATACTCCATTTTCAAAATTCTTTAAAAATGCTGGTGGATTTGCTTTTGGCTTTATGATGTCAGTGTTTGCTGGTTTTATTGCACATTCAATTGCAGGAAGACCAGGCTTAGCAGTCGGTATGGTAGCTGGAGCCATGGCATCTAGTACACAGTTTGCGGTTTATGAGACTGGTTCTAATGCAGGCTTCTTAGGAGCCTTAATTGGTGGTTTCATTGCCGGATTCTTAGTATTAGGATTAAAATGA", "aa": "MKIKELLTLKNIDINAKAKDKLEAIELAVNLMGDNIIIDKNAYLTAVLEREKQTTTGLGDELALPHGRGSFVKKPHLSALVLKDGVDYQSLDNKPVKLLFLIAAPETTDSENLHLEVLAKLSKMLVDKVFIEKLINAKSNKEFLKLIETKEQEVDQNEFEQTNNGEKFIVAVTACPTGIAHTYMAQESLENAAKEMGYQIKVETQGSGGAKNVLTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLK", "aa_hexdigest": "88cc80764c7173943f72682671b3f028", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00047C8194", "query_cov": 1.0240963855421688, "subject_cov": 0.9361233480176211, "identity": 0.511, "score": 363.0, "evalue": 4.02e-119, "valid": false, "product": "fructose PTS transporter subunit IIA", "uniref50_id": "UniRef50_A0A3B9ID08" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 653961, "stop": 655511, "observations": { "mutation": [ 654254, 654266 ], "stop": [ 654254, 654266 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XC71", "query_cov": 1.0, "subject_cov": 0.615727002967359, "identity": 0.973, "score": 758.0, "evalue": 1.91e-271, "gene_start": 1, "gene_end": 415, "reference_start": 1, "reference_end": 415, "reference_sequence": "MKIKELLTLKNIDINAKAKDKLEAIELAVNLMGDNIIIDKNAYLTAVLEREKQTTTGLGDELALPHGRGSFVKKPHLSALVLKDGVDYQSLDNKPVKLLFLIAAPETTDSENLHLEVLAKLSKMLVDKVFIEKLINAKSNKEFLKLIETKEQEVDQNEFEQTNNGEKFIVAVTACPTGIAHTYMAQESLENAAKEMGYQIKVETQGSGGAKNVLTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLKWCFKWLPKSLDGLKPMLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGALLAGMMAADMGGPVNKVAYTIGVASVDPDGINQPWIMAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGAIPYAASDPLRVIGSSIFASALAGGMAAGLGCSIPAPHGGIFVFVVAQGWYWYLLALAVGAFAGAFILGALRKKVDPSELGRWKGIPIGNGVYLKRKAKQTQKVEK" }, "paralog": false, "identity": 0.9728682170542635, "score": 970.0, "evalue": 0.0, "description": "Internal stop codon at 654254, 654266. Nonsense mutation around 654254, 654266.", "psc": { "uniref90_id": "UniRef90_A0AB32XC71", "product": "PTS system protein", "uniref50_id": "UniRef50_A0A1I6IS36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XC71", "UniRef:UniRef50_A0A1I6IS36" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1I6IS36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I6IS36" ], "product": "PTS system, fructose-specific IIC component" } }, "id": "IGIGPGHMMC_1168", "locus": "GCA000148625_01156" }, { "type": "cds", "sequence": "contig_1", "start": 655508, "stop": 656422, "strand": "-", "frame": 1, "gene": "pfkB", "product": "1-phosphofructokinase", "db_xrefs": [ "EC:2.7.1.56", "GO:0008662", "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0", "UniRef:UniRef90_C4XG41" ], "nt": "ATGATTTATACAGTGACTTTTTCACCTGCTATTGACTATACAATGTCTTTAGAGGGAAAAATAAATACAGGAGATATTAATCGTTCGAAAAGTGAAAATTATACTGTTGGTGGTAAAGGAATTAATGTTTCAATACTATTAAATAATTTAGGAACTAAAAATGTAGCTTTAGGTTTTGTTGGTGGTTTTACTGGTCAATATATTATTAATTATTTAAAAGCTAAAAAAATCAAAACAGATTTTTGTAATATTGAAGGTAATTCAAGAATTAATGTAAAACTAAATGGCAAAGCTAAAGAAACAGCTATTAATGCTAATGGGCCTAAAATCAGTGATCAAAATTTAAAACAATTATTAGATAAGATTGCTTCTAATATTAAAGATAATGATACTTTAATTTTAGCTGGCAATGTTCCAAAAAATGTTGACACAAATGTTTATGAATTAATTTTAGAAAAACTCAAAAACAAAAGAATTTATATAATAGTAGATTCAACTAAACAATTTCTACTTAATACATTAAAATACAAACCATTTTTGATTAAACCTAATTTAGAAGAACTTGAAGAATTATTTGATGAAAAAGTTAAAACTAAAGAAGACATAAAAAGTCATGTTGAAAAACTTAAAAAATTAGGTGCCAGAAATGTGTTAGTGTCTTGTGGCGCTGAAGGAGCTTATTTATTTAGTGAAGATAATCAAACCTTTTATAGTGCTACATATAAAGGAAAAGTTGTAAGTACAGTTGGTTCAGGAGATAGCATGATTGCTGGATTTATTGATAATTATTTAAAAAATAAAGATTATAAACAAGCTTTAGAATTCTCAATAGCTTGTGGAGCAGCAACTGCTTTTGATAAAGAAATAGCTAATAAAAGTGCAATATTAGAATTACTTGAAAGGGGTCAAAAGTGA", "aa": "MIYTVTFSPAIDYTMSLEGKINTGDINRSKSENYTVGGKGINVSILLNNLGTKNVALGFVGGFTGQYIINYLKAKKIKTDFCNIEGNSRINVKLNGKAKETAINANGPKISDQNLKQLLDKIASNIKDNDTLILAGNVPKNVDTNVYELILEKLKNKRIYIIVDSTKQFLLNTLKYKPFLIKPNLEELEELFDEKVKTKEDIKSHVEKLKKLGARNVLVSCGAEGAYLFSEDNQTFYSATYKGKVVSTVGSGDSMIAGFIDNYLKNKDYKQALEFSIACGAATAFDKEIANKSAILELLERGQK", "aa_hexdigest": "e564bd7086bee85895f02bd8d1dfdcc0", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XG41", "query_cov": 1.0, "subject_cov": 0.9934640522875817, "identity": 0.987, "score": 572.0, "evalue": 1.16e-205, "valid": true, "gene": "pfkB", "product": "1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_A0A3P8KBW0", "go_ids": [ "GO:0008662" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KBW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0" ], "product": "1-phosphofructokinase" }, "genes": [ "pfkB" ], "id": "IGIGPGHMMC_1169", "locus": "GCA000148625_01157" }, { "type": "cds", "sequence": "contig_1", "start": 656566, "stop": 657309, "strand": "-", "frame": 2, "gene": null, "product": "HTH deoR-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354800.1", "SO:0001217", "UniParc:UPI0001E32EF1", "UniRef:UniRef100_UPI0001E32EF1", "UniRef:UniRef50_C4XG42", "UniRef:UniRef90_C4XG42" ], "nt": "ATGCTTAAAGAACAAAGACTAGATGTTATTTTAAAAGAAGTAAATTCAAAAGGTGCTGCTTCACTAAAAGAGCTTGCAAAATTAACTCAAGCAAGCATTTCTTCAATACGCGAAGATGTTGCTGAATTAGATAAACAAAAATTATTAAAAAGAGTTCATGGCGGAGCTTATTCATTAAATAATAATTCTTCAGCAAATGATGTTGAATATGAATCAAGAAGTAATATTGAATTAGATGCAAAAATCAAATTAGCTACCAAAGCAAATGAATTTGTACAAGATAACTTATTAATTTTTATTGACTCAGGAACAACAACTCATCAATTAGCTAAATTAATTAATAATAAGAATGTAACTATAGTTACAAATTCAATTAATGTTGCTACAGAATTAAAAAGTCATCCAAACGTCACAACTATTTTAATAGGTGGATTATTAAAAAGTAATACATTTAATGTTGTTGGATCATTAGCTTTAGATAATTTGAAAAACTTTAATTTTGATATTGGATTTATTGGAGCAAATGGATATAGCAATAATCTAGGTTTTACAACACCTGAAATTAATGAAAGTTTATTTAAAAAAGAGGTCATTTCAAAATGCAAAAAAAGTTATATTTTGTTAGACAAAACGAAAATAAATTCTGAATTTAAATATACTTTTGCAACCAATAATAAAAACATTACATTGATAACTAATTACAAGAAAAAAGATCTAAATCTTGAGAACATTATAGAAGTATAA", "aa": "MLKEQRLDVILKEVNSKGAASLKELAKLTQASISSIREDVAELDKQKLLKRVHGGAYSLNNNSSANDVEYESRSNIELDAKIKLATKANEFVQDNLLIFIDSGTTTHQLAKLINNKNVTIVTNSINVATELKSHPNVTTILIGGLLKSNTFNVVGSLALDNLKNFNFDIGFIGANGYSNNLGFTTPEINESLFKKEVISKCKKSYILLDKTKINSEFKYTFATNNKNITLITNYKKKDLNLENIIEV", "aa_hexdigest": "536aceab204b040ec145e5c0f8ee5246", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EF1", "ncbi_nrp_id": "WP_013354800.1", "uniref100_id": "UniRef100_UPI0001E32EF1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EF1", "RefSeq:WP_013354800.1", "UniRef:UniRef100_UPI0001E32EF1" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32EF1", "uniref90_id": "UniRef90_C4XG42" }, "psc": { "uniref90_id": "UniRef90_C4XG42", "product": "HTH deoR-type domain-containing protein", "uniref50_id": "UniRef50_C4XG42" }, "pscc": { "uniref50_id": "UniRef50_C4XG42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG42" ], "product": "HTH deoR-type domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1170", "locus": "GCA000148625_01158" }, { "type": "cds", "sequence": "contig_1", "start": 657757, "stop": 657909, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAAGGATGTTAGAGCGCTTTCATATGACTTAGTTTTAAATGGTTTTGAATTAGGTTCAGGTTCAGCAAGAATATTTGATAAAAAAACACAAAAGAAAATGTTTGATTTAATTGGTATGAGTGAAAAAGAACAACAATCAAGATTTGATTGA", "aa": "MKDVRALSYDLVLNGFELGSGSARIFDKKTQKKMFDLIGMSEKEQQSRFD", "aa_hexdigest": "c931cfcca338c9288d5a96698176e035", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 47, "aa_cov": 0.94, "hmm_cov": 1.0, "evalue": 6.4575746557922355e-12, "score": 41.11616897583008, "start": 3, "stop": 49 } ], "seq_stats": { "molecular_weight": 5759.5484, "isoelectric_point": 8.065204429626462 }, "id": "IGIGPGHMMC_1171", "locus": "GCA000148625_01159" }, { "type": "cds", "sequence": "contig_1", "start": 658033, "stop": 658662, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTGTTCAGGTTTTGAAAAATATTATCAATTTGCAAGATGTTTCAGAGATGAAGATAGTAGAAAAGATCGTCAACCAGAATTTACTCAATTAGATATTGAAACTTCATTCTTAAATGTTGAAGACTTTCAAAAATACATTGAAAAATTATTCAAACACATTTTTAAATCAATAGGAATTAAAATCAAGACTCCATTTCCAAGAGTTAAATATTTTGATGCTTTAAGAGATTATGGAACAGATAAGCCTGACTTAAGATATGATTATAAAATAGTTGACATCAATGACTTTTGCCTTAATACAGACTTTAATATCATTAAAGATGCTCCAAGTAAAAGAATGCTTTTTGTAGATACTTTAATTACCAAAAAAGACTTTAAAGACTTAGAAGAAATTGCTAAGAAAAATAAAGCAAATATTCTATTCTACTTTGTTTATGAAAATGGTGAAATAGTTCACACCAACTTTGCAAACAAAGTTAAAGATGCAGCTTTAGAATTAGTCAAAAATAACAAAAACAAAAATGGTTCTTACTTTATAGTTGCTAATACTTATGATCACGCATCTCAAGCTTTAGGTGCAGTTAGAGTTGAATTAAATGAAAGATTTAATTATGCAAGAAAGGAATGA", "aa": "MCSGFEKYYQFARCFRDEDSRKDRQPEFTQLDIETSFLNVEDFQKYIEKLFKHIFKSIGIKIKTPFPRVKYFDALRDYGTDKPDLRYDYKIVDINDFCLNTDFNIIKDAPSKRMLFVDTLITKKDFKDLEEIAKKNKANILFYFVYENGEIVHTNFANKVKDAALELVKNNKNKNGSYFIVANTYDHASQALGAVRVELNERFNYARKE", "aa_hexdigest": "6b59aa2733468bdb42b44a3675d8fca1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 184, "aa_cov": 0.8516746411483254, "hmm_cov": 0.75, "evalue": 2.443470171694687e-34, "score": 114.75784301757812, "start": 1, "stop": 178 } ], "seq_stats": { "molecular_weight": 24781.913000000015, "isoelectric_point": 8.545236015319826 }, "id": "IGIGPGHMMC_1172", "locus": "GCA000148625_01160" }, { "type": "cds", "sequence": "contig_1", "start": 658702, "stop": 659145, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGACTTTTATTGACTTAAGAGACCGCTATGGAATTACCCAATGTGTCTTTAAAACAGACTTAAACGTCACAAAAGAAAGTTCAATGGAAATAATAGGGAAGGTTGTTTTAAGAAAACAAAAGAACCCTAATCTAGCTACTGGTGATATTGAAGTAGTAGTTGATAAATACACAATTTTTTCTGAAGCAAATGAAGAATTACCTTTTGCAATTAGAGATGATATTGATGTTAAAGAAGAAACCAGAATGAAATATCGTTTCTTAGATTTGCGTCGTCCAGTAATGCAACAAAACATTATCACTAAAGATAAAATTATGTTTGCTGTTAGAGAATTCATGCACCAAAATGATTTTATTGATATTGAAACTCCAATGCTTGCTAAAAGTACACCTGAAGGTGCTAGAGATTTTTTAGTACCTACTAGAAATAGAGATGAATTTTGA", "aa": "MTFIDLRDRYGITQCVFKTDLNVTKESSMEIIGKVVLRKQKNPNLATGDIEVVVDKYTIFSEANEELPFAIRDDIDVKEETRMKYRFLDLRRPVMQQNIITKDKIMFAVREFMHQNDFIDIETPMLAKSTPEGARDFLVPTRNRDEF", "aa_hexdigest": "d194834de10f293c2257d999412fb94c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 68, "aa_cov": 0.4557823129251701, "hmm_cov": 1.0, "evalue": 4.51219665090128e-23, "score": 77.75521850585938, "start": 78, "stop": 144 } ], "seq_stats": { "molecular_weight": 17299.730699999993, "isoelectric_point": 5.311058235168456 }, "id": "IGIGPGHMMC_1173", "locus": "GCA000148625_01161" }, { "type": "cds", "sequence": "contig_1", "start": 659251, "stop": 660243, "strand": "-", "frame": 2, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_A0A0F5H223", "UniRef:UniRef90_C4XG44" ], "nt": "ATGTTTCGTTACGAACAACCACAAAAAGGACGTTATAGACAATTTAATCAAGCAGGGATGGAAATAGTTGATACAGTTATTAATCCATATAATGATGCTGAATTAATTATTGCAGCAGCTTCACTATTACAATGTCTTGATGTTAAATGTGTTTTAAAAATTAATTCAATTGGTGATGAAGAAACTAGAACTAGATATCAAGAAGAATTAAAAAAATATTTTGCTAAATATAAAGATCAATTGAATCCAATTAGTCTTGAAAGACTTGAAAATAATGTTTTAAGAATTCTTGATGATAAAGAAGAAAGCAAAAAAGATTTTGTTAAAAAAGCTCCTAAAATTAATAAATATTTAAGCAAAAATAGTACTGAATTTTTCAATAAATTAACTTCAATTTTAGACTTTAACAATATCAAATATCAAGTTGATTATTCTTTAGTTAGAGGACTTGATTATTATGATGAAGTTGTTTATGAATTTGTTTCAACTTCAAAAGAATCAGGAAGTCAAAGTACAATAATTGGCGGTGGAAGATATTCGAACTTAATCAAAGAATTAGGTGGACCTCAATTATATGCTTGCGGTTTTGGTTTTGGAGTAGATCGAGCAGCTGACATTATTAATGATGAAAACAAAGACAATAATGATGCTGAAAAAATAAACATTTTAGTAGCCACAACCAGTGAAAAAAATCTAGACATTTTATTTTCATTAACTAATGAGTTAAGAAATTATGGAAGCAAAATTGAATTTATTAAAGAAGTAGTTAAAAGCAAAAAAATCTTTGATAAGGCTCAAAAATTACAAGCTGATGTTGTGATTTTTGATGACAACATTCAAGGTCGTGAAACCTTTGTTGCTAAAAGTTTAAGCGATAATGATCGTATTATGTTCCAATACAATGAAGATGGTTATGCAGACTTATTAGACTTTTTAGCAGAACATAATTTACTAAGCGATTTAATAGCTGAAGAAGATGAAGACGAGGAATAA", "aa": "MFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEESKKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE", "aa_hexdigest": "1a772db15bafbc9ba3774da1d907872a", "start_type": "ATG", "rbs_motif": null, "genes": [ "hisS" ], "truncated": "3-prime", "pseudogene": { "start": 659251, "stop": 660543, "observations": { "mutation": [ 660288 ], "stop": [ 660288 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG44", "query_cov": 1.0, "subject_cov": 0.7268722466960352, "identity": 0.9940000000000001, "score": 625.0, "evalue": 5.06e-224, "gene_start": 1, "gene_end": 330, "reference_start": 125, "reference_end": 454, "reference_sequence": "MYIIFNIKLSIYFRRHKMFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENKWYATLKNTKFAYYGPMFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEERQKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE" }, "paralog": false, "identity": 0.9930232558139535, "score": 843.0, "evalue": 2.01e-312, "description": "Internal stop codon at 660288. Nonsense mutation around 660288.", "psc": { "uniref90_id": "UniRef90_C4XG44", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A0F5H223", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG44", "EC:6.1.1.21", "UniRef:UniRef50_A0A0F5H223", "KEGG:K01892", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H223" ], "product": "Histidine--tRNA ligase" } }, "id": "IGIGPGHMMC_1174", "locus": "GCA000148625_01162" }, { "type": "cds", "sequence": "contig_1", "start": 660286, "stop": 660564, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "ATGTTTAACCGAATTAAAGGAACAAGAGACTTTGGTCCACAAGAAAATAATGTGATTGAATTAATTAGAAGTGCATTTGTTAATTATGCAAGAAATTATAATTTCCATTTAATTGAAACTCCAATTATTGAATCAGCCACTCTTTATCGTCGTTCAGTTGCAAGTAGCGATATAGTTAAAAAAGAAATGTACGAATTTAAAGACAAAGGTGATCGTGATATTGCACTAAGACCAGAAGGAACAGCAGGTTTTGTTAGAGCTTTAGTTGAAAACAAATGA", "aa": "MFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENK", "aa_hexdigest": "9a6234ea33c88e0ae5f1c82fd56bb223", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 84, "aa_cov": 0.8913043478260869, "hmm_cov": 0.9404761904761905, "evalue": 5.629314333215839e-10, "score": 34.91695785522461, "start": 7, "stop": 88 } ], "seq_stats": { "molecular_weight": 10642.96799999999, "isoelectric_point": 9.045768547058106 }, "id": "IGIGPGHMMC_1175", "locus": "GCA000148625_01163" }, { "type": "cds", "sequence": "contig_1", "start": 660730, "stop": 660903, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTGCTGCTATGCTAATAGTAATTAGTGGTAGCAATGGCGCTGGTATTTATTTCCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATTTTTTGCTGA", "aa": "MKKAKDNLNQKVNKRKKISFFAAMLIVISGSNGAGIYFRSESILNNSHQSLILAIFC", "aa_hexdigest": "c2974d3a67bc88aad98c97c5ba3ebe8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6359.448499999997, "isoelectric_point": 10.227280616760254 }, "id": "IGIGPGHMMC_1176", "locus": "GCA000148625_01164" }, { "type": "cds", "sequence": "contig_1", "start": 661027, "stop": 661167, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAAACTTTATGACTTATTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTGCAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCATTTGTTATTGGCACTAAATATGATTGA", "aa": "MSKNFMTYLYLPLSYFLMPLYALMSLQQGVGAILNQEAFVIGTKYD", "aa_hexdigest": "13da91fbf543d50881c93dd170be8092", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5285.2212, "isoelectric_point": 5.946972846984862 }, "id": "IGIGPGHMMC_1177", "locus": "GCA000148625_01165" }, { "type": "cds", "sequence": "contig_1", "start": 661261, "stop": 661644, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "ATGTTGGCTATTAAATTTTTGCCTTTATTCTTTATTATCATTATCGGTTTTGTTTTAGCTTTTAGTTCAAAAGGCGGAGTAAATCAAACTCAATGGAATTTACAAGATCAACACTTTGATGTTAGATTAGGAAGTTCAATTGCTTCTTATAAATTTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATTTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCTATTTCAATGAGTATTAATGGTGGCTTATTTGTTAAAATGAAAGATTATTTAAGTAACTGA", "aa": "MLAIKFLPLFFIIIIGFVLAFSSKGGVNQTQWNLQDQHFDVRLGSSIASYKFLGAGFGVFLSVSAIFFVYDGFYISAGIQSEMKEPQKTPLALLFGLLITTIIYLLIAISMSINGGLFVKMKDYLSN", "aa_hexdigest": "d46ebc26885c23e85ec626ef81fe3381", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 115, "aa_cov": 0.8740157480314961, "hmm_cov": 0.9217391304347826, "evalue": 1.4720997565087945e-08, "score": 29.77081871032715, "start": 3, "stop": 113 } ], "seq_stats": { "molecular_weight": 14048.490499999985, "isoelectric_point": 8.96376476287842 }, "id": "IGIGPGHMMC_1178", "locus": "GCA000148625_01166" }, { "type": "cds", "sequence": "contig_1", "start": 662146, "stop": 662406, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGTAATAATCACAACAATTATTTCTTTTATTGATTTATTTTTATTGATTGGTTTTGACAAGGAATACTTTAAAACAGTCGAATTGGAAAAATTTGCAAAAGACAGTACTTACATAATTAGAAATGAAAATACATTCTTTAATCAACTGCTTATAGGTAGAATTTTATGTGTTGTTACATTAATAATTTATTTACTTATTTCAATATTGCCAAGTGTTTTTGAAAAGATCTATTTTAAGAAAATGTTTAAATCATGA", "aa": "MSVIITTIISFIDLFLLIGFDKEYFKTVELEKFAKDSTYIIRNENTFFNQLLIGRILCVVTLIIYLLISILPSVFEKIYFKKMFKS", "aa_hexdigest": "86e159adcff65bd5e4ac743d3e299b74", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10156.135499999993, "isoelectric_point": 8.759013175964355 }, "id": "IGIGPGHMMC_1179", "locus": "GCA000148625_01167" }, { "type": "cds", "sequence": "contig_1", "start": 662522, "stop": 662893, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTTAATTCCCGGAGGTGGAGAGGCAAATATAAAATATCATAAAGGTAAATTTGTTACGGGAGATAATAGAATTGGACAAGTTCTAAATAAAAATGAAGTTGCAACAAAATTTTTATATTATTACTTTCTTTTAAATCTTGATTTAATTAGAAAAAATTTTAGAGGTGGTAGTATAAAGCATCCGTTTATGAAAAATATTTTGGAATTAAATATACCTATTCCTCCTTTAGAAACCCAAAACAAGATAGTTTCAATTCTTGACAAATTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAACTATTAGATTTTAAATAA", "aa": "MLIPGGGEANIKYHKGKFVTGDNRIGQVLNKNEVATKFLYYYFLLNLDLIRKNFRGGSIKHPFMKNILELNIPIPPLETQNKIVSILDKLSEYSQEINSGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "75cec985883856b72d421d248bccf76d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.9896907216494846, "evalue": 4.726138932848934e-19, "score": 65.21882629394531, "start": 10, "stop": 106 } ], "seq_stats": { "molecular_weight": 14264.403199999984, "isoelectric_point": 9.396927833557129 }, "id": "IGIGPGHMMC_1180", "locus": "GCA000148625_01168" }, { "type": "cds", "sequence": "contig_1", "start": 663593, "stop": 663733, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTACAAAAGTTATTAGATAAGTATTGTCCAGATGGATATGAGTGGGTTACATTGGGCGAAATATCAAGCATTAGGAGAGGTGCCTCTCCTAGACCTATATCTTCTTTTTTGTCTAAAGAAGGTTATCCATGA", "aa": "MSYLQKLLDKYCPDGYEWVTLGEISSIRRGASPRPISSFLSKEGYP", "aa_hexdigest": "7ae243b45b3552fb620c88577b9b2e05", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5239.9313999999995, "isoelectric_point": 7.869220542907715 }, "id": "IGIGPGHMMC_1181", "locus": "GCA000148625_01169" }, { "type": "cds", "sequence": "contig_1", "start": 663767, "stop": 664267, "strand": "-", "frame": 1, "gene": null, "product": "HTH domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "nt": "ATGATTTCTATATTTAAAGATAGAATTGAAATTGATTCTATGGGAAATTTAGGACCTGAACAAACAATAGAAGGTTTTTACAAAGGAGAATCAAAACCAGTTAATGAAAAGTTAGCCGAAATTTTTCTTCAATTAAGAATTAGTGAAAAATCAGGACGTGGAGTACCTGCAATAGTAAAAGTTTATGGCAAAGAAGCTATTTATTTTGGTGATGGATTTATTAAAGTTACAATTCCTTTTAATAGAATTAATGCTGTAAAATTTGAAATTGAAGTTGAAGAGATAGATGAAGAAACAGCTAAAAAAATTCTTAATCAAATAAGAAAAAAGATATTATCGGAAATTATTAAAAATCCACATATTACTAAAGCTTTATTAGCAGAAAAATTAAATCTAAGCACAACAGCAATAGACAACAATATTAATTTTTTAAAAAAACATGGTTTTATTAAAAGAGTTGGAGCAAATAAAAATGGTTATTGGAAGGCATTAATAAAATAA", "aa": "MISIFKDRIEIDSMGNLGPEQTIEGFYKGESKPVNEKLAEIFLQLRISEKSGRGVPAIVKVYGKEAIYFGDGFIKVTIPFNRINAVKFEIEVEEIDEETAKKILNQIRKKILSEIIKNPHITKALLAEKLNLSTTAIDNNINFLKKHGFIKRVGANKNGYWKALIK", "aa_hexdigest": "a49de4b4edd527e5d86e6c1466d6061b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X8GT79", "query_cov": 0.9879518072289156, "subject_cov": 1.0123456790123457, "identity": 0.573, "score": 176.0, "evalue": 7.64e-54, "valid": false, "product": "Winged helix-turn-helix DNA-binding protein", "uniref50_id": "UniRef50_D3MT65" }, "pscc": { "uniref50_id": "UniRef50_D3MT65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "product": "HTH domain protein" }, "genes": [], "id": "IGIGPGHMMC_1182", "locus": "GCA000148625_01170" }, { "type": "cds", "sequence": "contig_1", "start": 664286, "stop": 664543, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATAATTCTGGATTTCCAGTTAGAGTTTCTATTTTTGGAGGTTATGACAAAACAACCCCTTTATATACAGTAGATGAATTTGGATTTACTAATTTACTTTATACACTAGATGATTTGATTAGATTTGGAAATGTAGTAAATATTTATCAAGCTGATGAAAGAAACAGAGTTCTCACAAGAAAAGAAGTACCTTTATTTGATTCTAATGTTTACAAGGAAGCAATAATTAATGCCATCTTACATAACAAATGA", "aa": "MSDNSGFPVRVSIFGGYDKTTPLYTVDEFGFTNLLYTLDDLIRFGNVVNIYQADERNRVLTRKEVPLFDSNVYKEAIINAILHNK", "aa_hexdigest": "cda976f63e031c812f605beb8c012c8d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9763.917699999993, "isoelectric_point": 5.173166847229003 }, "id": "IGIGPGHMMC_1183", "locus": "GCA000148625_01171" }, { "type": "cds", "sequence": "contig_1", "start": 665313, "stop": 665804, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "nt": "ATGTCAGTTGTATTACAAGATTCATTTATGTTTAATGAAACCATTATGGACAACTTAAAAGTAGCTTCAGACAAAATCACAAACCAAGAAGTATATGAAATGGCTAGTTTAACTTCAGCTCATAACTTTATTCAAAAACTTGAAAAAGGTTATGACACTGTAATAGAAAATAGTGGTCAAAGTTTAAGTCAAGGTGAACGTCAATTGTTATCAATTACTCGGGCACTGTTGGGTAAAAAGAAAGTATTGATCTTAGATGAAGCCACAAGTAATGTTGATTCAAATACTGAACAAATTATTCAAAAAGCTTTACAAGAAGAATTAATGAAAGATCGTACTTCAATAGTTATAGCTCACCGTTTAAGTACTATTAAAAATGCAAACTTAATTTTAGTGGTTGATGACGGTCAAATTATTGAACAAGGAAATCATGCATCATTAATGGAAGCAAAAGGTTATTACTTTAATTTATATGAAAATCAATTCAAATAA", "aa": "MSVVLQDSFMFNETIMDNLKVASDKITNQEVYEMASLTSAHNFIQKLEKGYDTVIENSGQSLSQGERQLLSITRALLGKKKVLILDEATSNVDSNTEQIIQKALQEELMKDRTSIVIAHRLSTIKNANLILVVDDGQIIEQGNHASLMEAKGYYFNLYENQFK", "aa_hexdigest": "1de566babe198d621ff6c32fbffe7753", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00261D2CD4", "query_cov": 0.9938650306748467, "subject_cov": 0.9700598802395209, "identity": 0.556, "score": 172.0, "evalue": 5.31e-52, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A257LJQ8" }, "pscc": { "uniref50_id": "UniRef50_A0A257LJQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1184", "locus": "GCA000148625_01172" }, { "type": "cds", "sequence": "contig_1", "start": 666381, "stop": 666620, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTGTTGGTTTCACTTTAACAGCAATAGTAATTCCTTTATCGGTTTTATTCTTCTCGCTTGGCTACTTTATGATTTCAAAAGCTAGAAAATCATATGTTCTTGTACAACAAGACTTTGGTGATTTAAATGCTTACGTTGAAGAAATGCTAACTAATAGCAAAATCACTCAAACTTTTGATCGTCAAGAAACAGCAGAAAAGAATTTCAAAAAAATAACTCATGATATTTATTGA", "aa": "MAFVGFTLTAIVIPLSVLFFSLGYFMISKARKSYVLVQQDFGDLNAYVEEMLTNSKITQTFDRQETAEKNFKKITHDIY", "aa_hexdigest": "fdfdb893b78e48f911fdba37f6ccc169", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9124.468399999996, "isoelectric_point": 6.541281318664551 }, "id": "IGIGPGHMMC_1185", "locus": "GCA000148625_01173" }, { "type": "cds", "sequence": "contig_1", "start": 666657, "stop": 666785, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTTATTGAAATGCCAGTTAGCTTTTATGATAAAGAAAAAAGTGGTGACTTAATTTCAGTATTAATCAATGATATAAATAACTTAGCAAACTCATTTGTTCAAATGTTCAATGAAACTTGA", "aa": "MEKLIEMPVSFYDKEKSGDLISVLINDINNLANSFVQMFNET", "aa_hexdigest": "6178115e24ce4af06281e576f40eaf8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4838.4865, "isoelectric_point": 4.196388816833497 }, "id": "IGIGPGHMMC_1186", "locus": "GCA000148625_01174" }, { "type": "cds", "sequence": "contig_1", "start": 667168, "stop": 667566, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGCGTGAAGCACTTAAAAATGCTTGTGCTGATGATTTTGTTAATAAATTTGAAGATGGTTTAGATCACAAAGTTGTTCAAGGAGCTAGAAACCTTAGCGGTGGTCAAAAACAAAGAATTTCAATAGCTCGTAGTTTATTAAGAAAACCTAAAATCTTAATTTTAGATGATTCAACAAGTGCATTAGACAATATTACAACTAGAAAAGTTATTAATAATATTAAAAACAATTATGACTGTTCGACAATATTAATTAGCCAAAAAATTGGAGCTATTAAAAATGCTGACAATATTATGGTTTTAACTAATGGAGCAGTAATGGATCAAGGTAAACACGGTCACTTAATTAAGACCTGCGATTTTTACCAAAAGATCTATGAAACACAGCTAGAACAATAG", "aa": "MREALKNACADDFVNKFEDGLDHKVVQGARNLSGGQKQRISIARSLLRKPKILILDDSTSALDNITTRKVINNIKNNYDCSTILISQKIGAIKNADNIMVLTNGAVMDQGKHGHLIKTCDFYQKIYETQLEQ", "aa_hexdigest": "49ff5100b3412023d25897d9a9573dda", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.614, "score": 167.0, "evalue": 1.01e-50, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_1187", "locus": "GCA000148625_01175" }, { "type": "cds", "sequence": "contig_1", "start": 667594, "stop": 668190, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATTTTTGCTTTTGCTGTACCGATTTTTTATGCTTCAATTAACTTATTAATTATTGGAATCTATGCTTTTGCAGGTCATAGAATGTATACTGAAGTTGCAACTTTAGATTCACTTGTTAAAGTTAATATTTTTATAGAATACTTATTTTCAATTAGCTTTGGTCTCTTAATGACCATTCAATTCTTAACATCAATGTTTAGAGCTCGTGTTTCAGCAGGTAGAATTAATGAAATTTTAGAAACCAAAATAGATCCTTTATTTATTAAAGATGGTAAACAATTAACAAATAATTTTGATCTAGAAGTTCAAAATCTTTCATTTCGTTACTATGAAACCAGCCCTTCATATTCACTAATGAATATAAATGTTAAATTACCTTATAAACAAACTTTAGGTATTATTGGACCAACAGGTAGTGGTAAAAGTACATTTATTAACTTACTTTTAAATAATTATGTTTATGATGAAGGTTCAATTAAAATTGGAGGCCAAGAAGTTAAAGAAATTAATACCAAAAACCTTCATGAAACTGTTGGCATAGTTTACCAAGAAGCTTTACTTTACACTGGAACAATTAGATCAAACTTACTTTGA", "aa": "MIFAFAVPIFYASINLLIIGIYAFAGHRMYTEVATLDSLVKVNIFIEYLFSISFGLLMTIQFLTSMFRARVSAGRINEILETKIDPLFIKDGKQLTNNFDLEVQNLSFRYYETSPSYSLMNINVKLPYKQTLGIIGPTGSGKSTFINLLLNNYVYDEGSIKIGGQEVKEINTKNLHETVGIVYQEALLYTGTIRSNLL", "aa_hexdigest": "b7d2e9fbc18ce98e3055e89f9cb0be74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 77, "aa_cov": 0.3838383838383838, "hmm_cov": 1.0, "evalue": 8.24212734390871e-17, "score": 58.117149353027344, "start": 119, "stop": 194 } ], "seq_stats": { "molecular_weight": 22367.73180000001, "isoelectric_point": 6.737148475646973 }, "id": "IGIGPGHMMC_1188", "locus": "GCA000148625_01176" }, { "type": "cds", "sequence": "contig_1", "start": 668215, "stop": 668427, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTTAGTGTTTTAGCTGTTGTACCTGTAATTTTTGTACTAGTACTTGTTATTGGTTTAACTACTAATCCACTTTTAAAGAAAACACAAAAAGTTGTTGAAGAAATTACTAAAGCAACTGATGAAAATATTTTAGGAGCAAGAGTTATTAAAACTTTTAACTTAGAAGAAACAAGAAAAAAACGTTTTAGTGAATACAACCACAGATGA", "aa": "MSLSVLAVVPVIFVLVLVIGLTTNPLLKKTQKVVEEITKATDENILGARVIKTFNLEETRKKRFSEYNHR", "aa_hexdigest": "5035845b45203479abbec87401ce82ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.3474, "isoelectric_point": 9.773100852966305 }, "id": "IGIGPGHMMC_1189", "locus": "GCA000148625_01177" }, { "type": "cds", "sequence": "contig_1", "start": 668764, "stop": 668949, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAGTTAATTAAAATGCTACCAATTAAGATTAAATTAGCATTTCTTTTTGGTTCATTGCTTGTTTTTATTAATATTATTTTTACTTTGCTTTTGCCTAATATTATTTCTCAGTTTATTAGATTATTATTCGTTGATGATTATTCAAAAGTTGAGACATTATCCTTCTTTAATGGTCGTTGA", "aa": "MFKLIKMLPIKIKLAFLFGSLLVFINIIFTLLLPNIISQFIRLLFVDDYSKVETLSFFNGR", "aa_hexdigest": "316671a72d6d69cb3154fa8398c32412", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7154.733899999997, "isoelectric_point": 9.994614219665529 }, "id": "IGIGPGHMMC_1190", "locus": "GCA000148625_01178" }, { "type": "cds", "sequence": "contig_1", "start": 669292, "stop": 669525, "strand": "+", "frame": 1, "gene": null, "product": "Nudix hydrolase domain-containing protein", "db_xrefs": [ "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_C4XG50", "UniRef:UniRef90_C4XG50" ], "nt": "ATGAAAGAAAAATATGTTTTACCTAACGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATTCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGAAGAAAAAAAGAATAAATAA", "aa": "MKEKYVLPNGIPKEVVYFLAIQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLEEKKNK", "aa_hexdigest": "3196b853344da8971740918b81d97ec9", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 669064, "stop": 669525, "observations": { "mutation": [ 669079, 669142, 669178 ], "stop": [ 669079, 669142, 669178 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG50", "query_cov": 1.0, "subject_cov": 0.5032679738562091, "identity": 0.9740000000000001, "score": 145.0, "evalue": 3.5e-43, "gene_start": 1, "gene_end": 77, "reference_start": 77, "reference_end": 153, "reference_sequence": "MFQKKWGLNMSEKSCGLVIFRQFTKGWKVLIAKQINNMWSFVKGHVENKESEIETALREAKEEVNLENFQIFKETRMKEKYVLPNGIPKEVVYFLAMQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLDEKKNK" }, "paralog": false, "identity": 0.9607843137254902, "score": 290.0, "evalue": 1.56e-102, "description": "Internal stop codon at 669079, 669142, 669178. Nonsense mutation around 669079, 669142, 669178.", "psc": { "uniref90_id": "UniRef90_C4XG50", "product": "Nudix hydrolase domain-containing protein", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_C4XG50", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG50", "EC:3.6.1.17", "UniRef:UniRef50_C4XG50", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG50" ], "product": "Nudix hydrolase domain-containing protein" } }, "id": "IGIGPGHMMC_1191", "locus": "GCA000148625_01179" }, { "type": "cds", "sequence": "contig_1", "start": 669605, "stop": 670108, "strand": "-", "frame": 1, "gene": "pgmB", "product": "beta-phosphoglucomutase", "db_xrefs": [ "EC:5.4.2.6", "GO:0000287", "GO:0005975", "GO:0008801", "SO:0001217", "UniRef:UniRef50_A0A5B8JY41", "UniRef:UniRef90_A0A3P8K8F6" ], "nt": "TTGCCTCGTAGAGATACTTTACTTGCAATATTTGAATTAAAGAAATTTAAGCATAATTTTGATGATCAAAAAATCAATGAATTATGTACTAAAAAGAATGAATTATATTTAAGTTATTTAAAAAAATATATTAATAAAGATAGCCTTTTAGAAGGCATCGAACAATTTTTAAAAGACTTAAAAAAAGATAAGATTAAAATTGCAATAGCTTCTTCAAGTCTCAATGCTCCTTTGATTTTAGAAAAACTCGGAGTTATAAATTATTTTGATGTTATTGTTAATCCTGTTGAAGTTAAAAAAGGAAAACCTGCACCAGATATTTTTCTTTTAGCTCAAGAACAACTTAAATTAAAAAAAGAAGAATGTGTTGGAATTGAAGATGCAGTTGTTGGAGTTGAAGCTTTAAATAAGGCTAACATTAAATCCATTGCAATAACAAACGGTAATAATAATTTATTCCAAAAAGCAACTTTATTAGTTCAAGACACTTCTAAATTAATTTGA", "aa": "MPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLI", "aa_hexdigest": "62ea9c14ed358c8169efc4738cc1a76d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pgmB" ], "truncated": "both", "pseudogene": { "start": 669578, "stop": 670300, "observations": { "mutation": [ 669607, 670174, 670249 ], "stop": [ 669607, 670174, 670249 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F6", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.9940000000000001, "score": 318.0, "evalue": 1.55e-108, "gene_start": 1, "gene_end": 167, "reference_start": 65, "reference_end": 231, "reference_sequence": "MLMVWTVKLQMKSKFSRWKMIKGIIFDLDGVITDTAKLHYKAWKEIVNELGIDYSKEENEKLRGLPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLIWDLIKPILF" }, "paralog": false, "identity": 0.9833333333333333, "score": 454.0, "evalue": 7.61e-165, "description": "Internal stop codon at 669607, 670174, 670249. Nonsense mutation around 669607, 670174, 670249.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F6", "gene": "pgmB", "product": "beta-phosphoglucomutase", "ec_ids": [ "5.4.2.6" ], "uniref50_id": "UniRef50_A0A5B8JY41", "go_ids": [ "GO:0000287", "GO:0005975", "GO:0008801" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F6", "EC:5.4.2.6", "UniRef:UniRef50_A0A5B8JY41", "GO:0000287", "GO:0005975", "GO:0008801" ] }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JY41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JY41" ], "product": "Beta-phosphoglucomutase" } }, "id": "IGIGPGHMMC_1192", "locus": "GCA000148625_01180" }, { "type": "cds", "sequence": "contig_1", "start": 670542, "stop": 671207, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03632.21" ], "nt": "ATGAAAAAAATGGGTTATGAAATGATAATTGATACAGCTTACTTTTACACAAACCGTGCAGAAATACAACCAGATGGTAGTTATGAAATTAAAGATGTAATGGGTCCAAATGAATATAAAGGAAACATTAATAATAATGCATTTATCAACCAAATGGCCTTATTTAATATGAAATTGGCAATTTCAATATATCGCAAATTGCAAGCACAAACACCAGGCTTACTTAACTGTATTTTAAAAAGAATACCTTATAAAATTAATTTTTCAAAAATGACTAAAGTCATTAAAGGTCTTAAAATTCAAGCTCCTAATAAAGATAAAATTATTGCAGAAAATGATACATTCTTATCCTTACCTTTAAAAAATGTTAGACCATTCCAAATGCTTGGAGATGCTGGTAAAAAATTATTCAATACTTCTGAAGGACATAGATTATTAGGCTCTCAATTAGTAAAACAAGCCGATGTCGTTCTCTTAACTTATTTATTCCCTGAGTTATATAATAAAGAAATTAGAGAAAAAAACTTTGATTACTATGAAGCAATAACCACACATGATTCATCGCTTTCGGCAGCAACTTATTGTATTGAAGCTATTAGATTAAATAAAATTGAAAAAGCTTATGAATTATTTAAATATGGTATTAATATTGACTTTGGTCCATGA", "aa": "MKKMGYEMIIDTAYFYTNRAEIQPDGSYEIKDVMGPNEYKGNINNNAFINQMALFNMKLAISIYRKLQAQTPGLLNCILKRIPYKINFSKMTKVIKGLKIQAPNKDKIIAENDTFLSLPLKNVRPFQMLGDAGKKLFNTSEGHRLLGSQLVKQADVVLLTYLFPELYNKEIREKNFDYYEAITTHDSSLSAATYCIEAIRLNKIEKAYELFKYGINIDFGP", "aa_hexdigest": "607287770a93d446aae2381a3a082dcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03632.21", "name": "Glyco_hydro_65m", "length": 221, "aa_cov": 0.9909502262443439, "hmm_cov": 0.9638009049773756, "evalue": 2.1491328050930003e-56, "score": 187.43862915039062, "start": 1, "stop": 219 } ], "seq_stats": { "molecular_weight": 25394.259500000007, "isoelectric_point": 9.173480415344237 }, "id": "IGIGPGHMMC_1193", "locus": "GCA000148625_01181" }, { "type": "cds", "sequence": "contig_1", "start": 671709, "stop": 672605, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATATTGAATATGACCCTGATAATAATCAGATTTCACAGATTAAATTTGACCGTAATATAACAGCAAAAAGTGAAAGTATTTTTTCACTAGGTAATGGTTATTTAGGTATTAGAAGTGTTGATGAAGAAGTTCAAGAATATAACAAAGAAGACTTTTTTGTTAATGGTATTTTTAATAGAACATCTAAAGGAGAAGTTCCTGAATTAGCTAATTTAGCTGATTTAATTCAAACTCCTATTTCTTTTAATAATCAAATTTTTGAAGTTAATGAAAAGGATCAATATACTAAAACTTTAGATATTAAAAATGGAATTTTAAGTCGTAAAGTCATTGCCAAAAGAGACTTTGCTACAATTGAATTAAATTTTGAACGTTTTGTTTCTCAAGAAATAAGAAACATTTATGCTCAAAAAATTACAATTAAAATTTTAGATTCTAAAGATGAAATTGTTAAGGTTAAATTATTACCAGGAATTAATGGACAAGTAACAAACAGTGGTACTCAACACTTTGAAGAAGGTCTTAAAAACCGTGTGACAACCGAAAGTTTGAAGATGCAACAAATAACAACTAATTCAAATCGTTTTGTTGTGCACCACATGTTTACAAGACTTTTAAAAAATAATAAGTTAATTTTAGGCAGTAATGACAAATATCAAATTCAAACTGATCGCCGCAAAGTTTACTTTAAAATCGAAAATGAATTAAAAGCAAACGAAGAATTAGTTTTAGAAAAATTAATGTCAGTTCATACATCAATTGATGATGAAGAAAAAATATTAACTAATAGTGAAGTTTTGGCTAAATCAGATTTAACTTTTAACTACTTATTAAAAAATACATATGCTTCATTAAAAGAAAATTCTACAGCTGCAATGCAAAAAGTTTGA", "aa": "MEYIEYDPDNNQISQIKFDRNITAKSESIFSLGNGYLGIRSVDEEVQEYNKEDFFVNGIFNRTSKGEVPELANLADLIQTPISFNNQIFEVNEKDQYTKTLDIKNGILSRKVIAKRDFATIELNFERFVSQEIRNIYAQKITIKILDSKDEIVKVKLLPGINGQVTNSGTQHFEEGLKNRVTTESLKMQQITTNSNRFVVHHMFTRLLKNNKLILGSNDKYQIQTDRRKVYFKIENELKANEELVLEKLMSVHTSIDDEEKILTNSEVLAKSDLTFNYLLKNTYASLKENSTAAMQKV", "aa_hexdigest": "a1b45e26078bfdf17cfe32092316992b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34425.60590000004, "isoelectric_point": 5.952997779846191 }, "id": "IGIGPGHMMC_1194", "locus": "GCA000148625_01182" }, { "type": "cds", "sequence": "contig_1", "start": 672615, "stop": 672740, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACATCAAAAAGTAGTTGAAAATGTTCAAGGAAAACAAATATTATCAACATATATTGATAACAAAAAAGTTACTGATAAATTAAGACCTTTTGAGTCGATTATGTTAGAAATTAAATAA", "aa": "MTKHQKVVENVQGKQILSTYIDNKKVTDKLRPFESIMLEIK", "aa_hexdigest": "f0dee4995cbcbd89a904d5f347d56a20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.654999999999, "isoelectric_point": 9.52289905548096 }, "id": "IGIGPGHMMC_1195", "locus": "GCA000148625_01183" }, { "type": "cds", "sequence": "contig_1", "start": 673005, "stop": 673220, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00128.31" ], "nt": "ATGCTTTTTGGGCTAAAAGGTATTCCTTGTATTTATTATGGAGAAGAAATTGGTTTATTAAATAATCAATTTAACTCAAGAGAAGAATTTAAAGATTGTGACGTATTTAATGCCTTTAGTGAGTTAGTAGATAAAAAGAAAATTTACAGTGAATACGAATTTATTAAATATTGCAATATCAACAGTCGTGACTCAGGCCGCACTTTGATGCAATGA", "aa": "MLFGLKGIPCIYYGEEIGLLNNQFNSREEFKDCDVFNAFSELVDKKKIYSEYEFIKYCNINSRDSGRTLMQ", "aa_hexdigest": "d395cf91abcb537d585063c35ad7748a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00128.31", "name": "Alpha-amylase", "length": 70, "aa_cov": 0.9859154929577465, "hmm_cov": 0.7285714285714285, "evalue": 1.68890665952592e-07, "score": 26.82413101196289, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 8377.450499999997, "isoelectric_point": 4.990997886657714 }, "id": "IGIGPGHMMC_1196", "locus": "GCA000148625_01184" }, { "type": "cds", "sequence": "contig_1", "start": 673440, "stop": 673586, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCTTAAGTTTAATAAGTTAGCTTTTAGTGATAAAAAAGATGCTTATACATTAGGTGAAGCTAGTGGAATTAATTTAGAAGAATTATTAAAATATGGAACAGGATCTAAAAAAGTAGCTGATAACTATTACAACTTTTCTTGA", "aa": "MLLKFNKLAFSDKKDAYTLGEASGINLEELLKYGTGSKKVADNYYNFS", "aa_hexdigest": "b6d4506498b4c2104deb739d609a6e3c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.071800000002, "isoelectric_point": 7.961668205261231 }, "id": "IGIGPGHMMC_1197", "locus": "GCA000148625_01185" }, { "type": "cds", "sequence": "contig_1", "start": 674112, "stop": 674258, "strand": "-", "frame": 3, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "nt": "ATGAAAACAATAAAATTAGAAGATAAAATTATTTACCAAATATTTCCTCGTTCATTTTATGACTCAAATAATGATGGCGATGGAGACTTATTAGGTATAGCAAAAAAACTAGATTATTTACAAGACTTAGGTATAAATGCGATATGA", "aa": "MKTIKLEDKIIYQIFPRSFYDSNNDGDGDLLGIAKKLDYLQDLGINAI", "aa_hexdigest": "53bacd49ab9424190d5d678759b2dd5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FD1789", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.7709999999999999, "score": 80.1, "evalue": 5.15e-19, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI0021FD1789" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FD1789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "IGIGPGHMMC_1198", "locus": "GCA000148625_01186" }, { "type": "cds", "sequence": "contig_1", "start": 674602, "stop": 674808, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTGCAAATTACAACACAAGGACGTCAATTCTTTTTAGCAGGTACTGAATTAGCTCAAAGTAAACCTGCTGATAAAAGTGGTCAAGATTTTCATAAGTCATTTTATTCATATGCCAAAGATTATTTTGAATTAAATTCAGATGAAAATAAATACCATTTTAATTCTTATAAAACGACAGATTATACTAACGGACTAAAATGA", "aa": "MILQITTQGRQFFLAGTELAQSKPADKSGQDFHKSFYSYAKDYFELNSDENKYHFNSYKTTDYTNGLK", "aa_hexdigest": "14bc447c73774bc7e88605fb093b9d0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7929.621499999998, "isoelectric_point": 6.706910133361816 }, "id": "IGIGPGHMMC_1199", "locus": "GCA000148625_01187" }, { "type": "cds", "sequence": "contig_1", "start": 675193, "stop": 675465, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ], "nt": "ATGATGATAAATTCAATTTATGATAATATTCTGCCTGGTTACTATTATCGAGATAATGCTAAAGTAAAACCTGTTATTTATCCGCCTTTAGCAAGTCAAAGATATATGGTTAGAAAACTAATTTATGAATCACTAAAACATTTTGTTGAATATTATGATGTTGATGGTTTCCGTTTTGATTTATCAAGTTTTATTGATAAAGAAACACTTGATTATTGTTTTAAAAACTTAAGAAAAATAAAACCTAATTTAGTAATCCATGGTGAAGCTTGA", "aa": "MMINSIYDNILPGYYYRDNAKVKPVIYPPLASQRYMVRKLIYESLKHFVEYYDVDGFRFDLSSFIDKETLDYCFKNLRKIKPNLVIHGEA", "aa_hexdigest": "22ce29c00e40eccfa8031a13fb171c4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002540EC32", "query_cov": 0.9444444444444444, "subject_cov": 0.8854166666666666, "identity": 0.6940000000000001, "score": 123.0, "evalue": 7.28e-35, "valid": false, "uniref50_id": "UniRef50_UPI0021AF9781" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF9781", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10795.404299999991, "isoelectric_point": 8.62549915313721 }, "id": "IGIGPGHMMC_1200", "locus": "GCA000148625_01188" }, { "type": "cds", "sequence": "contig_1", "start": 675896, "stop": 675988, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCACATTTTGATTTATTATCTAGATTAACTATTGCGCCTTTTGCAATTTTATCTTCTTTTGCTTCTCAATCAAAAGGAGCTAAAGAATAA", "aa": "MPHFDLLSRLTIAPFAILSSFASQSKGAKE", "aa_hexdigest": "3031f9f05a36f871fbfc0eaf29d951e5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3263.7622, "isoelectric_point": 8.36672306060791 }, "id": "IGIGPGHMMC_1201", "locus": "GCA000148625_01189" }, { "type": "cds", "sequence": "contig_1", "start": 676183, "stop": 676299, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATAAAAGGACAAACTAAAGAATTTTTTAAGGACTTTGATGCTAAATATGCTTATAAAAAAGCTGATTTAGGAACAAAATTCCATAAAAATCATATTGAAATAAAATTTTGA", "aa": "MNIKGQTKEFFKDFDAKYAYKKADLGTKFHKNHIEIKF", "aa_hexdigest": "47b64872272290e3a6bff20cd568ba2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4581.255099999999, "isoelectric_point": 9.574667167663574 }, "id": "IGIGPGHMMC_1202", "locus": "GCA000148625_01190" }, { "type": "cds", "sequence": "contig_1", "start": 676445, "stop": 676798, "strand": "-", "frame": 1, "gene": null, "product": "HTH gntR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07", "UniRef:UniRef50_UPI0021AECA4B", "UniRef:UniRef90_C4XG55" ], "nt": "ATGGTAGATAAAAGAATTCTCTTTGTTCAAGGTTTTAGAACTTTTGAAAAAACTTATTATAAAGATAATGAGGTTTTAGCAACTTCTGAAATTTATGTAAGCTGTAAAAATATTGAAGTTGATGAGGAAATTAATTTTAATAAATCTTTAACCGACTTATTAATTGAGCGCGATGGTTTAAATAATATGGTTTATTCATTATCTTATGAAAATGTTAAAAAATTTGGATATGAAAGCTTGGTAGTTGTTTCATTTTTTGGCTATGATGAAGATAGCATTTCTGTAGCTGGAAAGTATTATATTAATCCTAAATACTTTAGATTTTTCCATCAAGAATTTTCATTAAAATCATAA", "aa": "MVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS", "aa_hexdigest": "63039c7ad349478c37a49bd95f022305", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0021AECA4B", "query_cov": 0.9658119658119658, "subject_cov": 0.8828125, "identity": 0.513, "score": 123.0, "evalue": 5.44e-34, "valid": false, "uniref50_id": "UniRef50_UPI0021AECA4B" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AECA4B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AECA4B" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 676445, "stop": 677098, "observations": { "mutation": [ 676801 ], "stop": [ 676801 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG55", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 232.0, "evalue": 1e-75, "gene_start": 1, "gene_end": 117, "reference_start": 118, "reference_end": 234, "reference_sequence": "MFLKFKGVYNIYIMDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQWMVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 427.0, "evalue": 5.82e-155, "description": "Internal stop codon at 676801. Nonsense mutation around 676801.", "psc": { "uniref90_id": "UniRef90_C4XG55", "product": "HTH gntR-type domain-containing protein", "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG55", "UniRef:UniRef50_L0RW07" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07" ], "product": "HTH gntR-type domain-containing protein" } }, "id": "IGIGPGHMMC_1203", "locus": "GCA000148625_01191" }, { "type": "cds", "sequence": "contig_1", "start": 676799, "stop": 677110, "strand": "-", "frame": 1, "gene": "gntR", "product": "GntR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "nt": "ATGGATTTACAAGAAAAGAAGAAAACACAAGTGATTATTGATTATTTAATTGATTTGATTAAAACTCAAAAAGTACCAACAAACAAAATCTTGCCAAGTGAGCATTCTTTAATGATGCGTTTTGATTGTTCAAGAAGTATTGTTGTTAAAGCATATCAAAAATTAGAAGCATTAGGAGCTATCTATTCAATTTCAAAAAGAGGACATTTTGTTGCTGAAAACTTTCACAACTTAATTAAACCAATTAGCTATCTTTTAGGAGCAACAGCTCAAGAAGGAAAAGAAATAAAAAGCAAGGAATTACCTCAATGA", "aa": "MDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQ", "aa_hexdigest": "31cec69671252884f422fec175262b5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF0E19D", "query_cov": 0.8058252427184466, "subject_cov": 0.8137254901960784, "identity": 0.711, "score": 127.0, "evalue": 2.17e-36, "valid": false, "product": "GntR family transcriptional regulator", "uniref50_id": "UniRef50_UPI0021E0F715" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0F715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "product": "GntR family transcriptional regulator" }, "genes": [ "gntR" ], "id": "IGIGPGHMMC_1204", "locus": "GCA000148625_01192" }, { "type": "cds", "sequence": "contig_1", "start": 677208, "stop": 677468, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF", "UniRef:UniRef90_UPI0001E32EFF" ], "nt": "ATGATTGCTTTAATTAATAAGCTAAGAAAAGAATATTCTTTTTTAAGTTTAACTAATATAGAAACTTTAGTTGATCCAAGATTAGTAGTAGACAGTAAAGATTATTCAAATGTTATTGTCCGTAAAGATCTTAATGATGATAAGAATTATTTAATGTTTGTTATTTACAATCATAAATGTCAAAATAATGTTCTTCAAAAGATTAGCAGGAATTATCACTTTGAAGCATTATATGAATATAAATGCAAAAATATGCCATGA", "aa": "MIALINKLRKEYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMP", "aa_hexdigest": "240dc424dbde29044516c44134e711c2", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 677154, "stop": 677714, "observations": { "mutation": [ 677210, 677603 ], "stop": [ 677210, 677603 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32EFF", "query_cov": 1.0, "subject_cov": 0.46236559139784944, "identity": 0.93, "score": 157.0, "evalue": 1.89e-47, "gene_start": 1, "gene_end": 86, "reference_start": 83, "reference_end": 168, "reference_sequence": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMKWKNHQEISAIYEKKLDACQSNLFLNVSNSLDYVENEIAPGNSIYNMIALINKLRKEYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMPWGIIMQKYACGIYKLTKK" }, "paralog": false, "identity": 0.9623655913978495, "score": 355.0, "evalue": 1.33e-127, "description": "Internal stop codon at 677210, 677603. Nonsense mutation around 677210, 677603.", "psc": { "uniref90_id": "UniRef90_UPI0001E32EFF", "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32EFF", "UniRef:UniRef50_UPI0001E32EFF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10336.878899999996, "isoelectric_point": 8.886080360412599 }, "id": "IGIGPGHMMC_1205", "locus": "GCA000148625_01193" }, { "type": "cds", "sequence": "contig_1", "start": 677601, "stop": 677714, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTATCTTTTAGCTTTGCCTGCTAAACCAATAATTTATTATGGTGATGAATTAGCATATGCAGGAACTAGAAGCTTTGATGATCCTGGTTTAAGAGAACCAATGAAGTGA", "aa": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMK", "aa_hexdigest": "389e5d2b2e414406c4f45697653c71b4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4189.8484, "isoelectric_point": 4.8610639572143555 }, "id": "IGIGPGHMMC_1206", "locus": "GCA000148625_01194" }, { "type": "cds", "sequence": "contig_1", "start": 679758, "stop": 680078, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAATAATATCATCAAAATTAGTTTTTTTTCTTAAAAAATCTTCTAACTAA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSIISSKLVFFLKKSSN", "aa_hexdigest": "920815c94b6ebd0c1000b79640b9735d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12364.57399999998, "isoelectric_point": 9.96186428070068 }, "id": "IGIGPGHMMC_1207", "locus": "GCA000148625_01195" }, { "type": "cds", "sequence": "contig_1", "start": 680217, "stop": 680345, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGTTATACTAACAAACTTTATTTTGTTAATTGTTGCTAAAACAAAATATGGATATGTTAAAAATGACCAAATTATTGCTAAAAGCAAAATTGATACTAAGGACTTAGAAAAACAATTAATATAA", "aa": "MFVILTNFILLIVAKTKYGYVKNDQIIAKSKIDTKDLEKQLI", "aa_hexdigest": "9a1f1b8edc24a089397c55dd38a791a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4870.8315, "isoelectric_point": 9.45688343048096 }, "id": "IGIGPGHMMC_1208", "locus": "GCA000148625_01196" }, { "type": "cds", "sequence": "contig_1", "start": 680352, "stop": 680609, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATAAATATTCTCTTCATATCTTTAATCATTAATTGTATTGCAGTAATTCGACTTTTCTCAAATAAAAGTAATGCAGACAAATACTTTATTTATCTTATATTTCTCCAATTAACCTCTTTTTGTTTATTCTTTTTTGCAATCTCACTAAGTCCTAATAAACTTAACAAAGTTATTGACTTTAAAGAATTAAGCGGTTCAGAATATGCTATATATCAATTTTTAGATAAAATGAACATTACTTATGCATGA", "aa": "MININILFISLIINCIAVIRLFSNKSNADKYFIYLIFLQLTSFCLFFFAISLSPNKLNKVIDFKELSGSEYAIYQFLDKMNITYA", "aa_hexdigest": "9cb1d343e5f21775a9d36a7a46107721", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9907.670199999991, "isoelectric_point": 8.617247200012208 }, "id": "IGIGPGHMMC_1209", "locus": "GCA000148625_01197" }, { "type": "cds", "sequence": "contig_1", "start": 680721, "stop": 680879, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAGTTAATCAAAAATTAAAAAAATCTAAAACAAATTATTTAGCTCGTAAATCTTTATTAGTATTATCAATTATTATTACAATAGTTGCAATAGCAATATTGTTTGCTTTTCTCTTTTTATTCTTAAAAAGAATAGATGAAGAATTAAATTGA", "aa": "MFKVNQKLKKSKTNYLARKSLLVLSIIITIVAIAILFAFLFLFLKRIDEELN", "aa_hexdigest": "c36ec00f5fb637ce26c7203e8208a0b5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6070.406899999995, "isoelectric_point": 10.214515876770019 }, "id": "IGIGPGHMMC_1210", "locus": "GCA000148625_01198" }, { "type": "cds", "sequence": "contig_1", "start": 680879, "stop": 682072, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1", "UniRef:UniRef90_C4XG58" ], "nt": "TTGAATAATACTCAAAATCAAAATGAAGATTTTGACGATTTATCCTTTGAAACAATTAATTTGGACAAAATGCTAGATGAAATCGGTGAGCTTAGAAATATTGAAAAAGGAGCTCATATTGAACTAAAAGGATTATCTAAAAAATATGAAGGTAATGAAAATTATACACTTGAAAATATTAATTTAGATATTCAACCAGGTAAATTTTGTATCTTTTTAGGACCTAGTGGTTGCGGTAAAACTACACTACTTAGAATGATTGCGGGATTAAATTCAATTACAAAAGGTGACTTATTATTCAATGGCAAAAGATATAATAACCTTTTACCTAGTGAAAGAAATATTGCAATGGTTTTTCAATCATATGCTTTATATCCTCACATGAACGTTTACAATAATATTGCTTTTGGTTTAAAAATAGCCAAAGAAAGAAAAGACGTTATTGACAAACGTGTTAAGGATGTTGCTAAAATTCTAAAAATAGAAGATTACTTATACAGAAAACCACGTGACTTATCTGGTGGTCAAAGACAACGTGTGGCAATAGGTCGTGCTATTGCTCGTAAACCTCAAGTGTTCTTGATGGATGAGCCTCTTTCTAACCTTGATGCAAAACTTCGTGAAAGTATGCGTCGTGAAATTGTTAATATCCACAGAATGCTTGGCGCAACCAGTATCTATGTAACTCATGACCAACTTGAAGCTATGACAATGGGTGATCAAATTGTTGTCTTCAATGATGGCTCAATTCAACAAAATGGTACTGGCCGTGACTTATACTTCAAACCTGCTAATGTCTTTGTAGCTAGATTTATTGGTTCTCCAACCATGAATGTTTTCAAAGCAGCATGTGAAAACGGCAAAATTGTTAGTGCTCAAAAAGACATTAAAATTAAAGCCCCAAAAGAATATTTGGAACAATTAAAAGACAAAAAATTAAAAATTGGTTTTAGAAGTGAAGACATCAAAATTCATGCTTCTGAAGTATCAAATTCAATTCATGCAACTATTAAAAATATTGAATTAATTGGTAAAGAACAACTTGTAACTGTTAAATTGGATAATGACGATATCGAACTTACAATTACAGCAAGCAATTCAGATACTTACGAGCTTTATTCAAAAGTTTACATTGAATTTTATGAACCTAGAATCCATATCTTCGATGCGGAAACAACAAAACGGATTAATTAA", "aa": "MNNTQNQNEDFDDLSFETINLDKMLDEIGELRNIEKGAHIELKGLSKKYEGNENYTLENINLDIQPGKFCIFLGPSGCGKTTLLRMIAGLNSITKGDLLFNGKRYNNLLPSERNIAMVFQSYALYPHMNVYNNIAFGLKIAKERKDVIDKRVKDVAKILKIEDYLYRKPRDLSGGQRQRVAIGRAIARKPQVFLMDEPLSNLDAKLRESMRREIVNIHRMLGATSIYVTHDQLEAMTMGDQIVVFNDGSIQQNGTGRDLYFKPANVFVARFIGSPTMNVFKAACENGKIVSAQKDIKIKAPKEYLEQLKDKKLKIGFRSEDIKIHASEVSNSIHATIKNIELIGKEQLVTVKLDNDDIELTITASNSDTYELYSKVYIEFYEPRIHIFDAETTKRIN", "aa_hexdigest": "6e4938bc3c00c1deea62cc35414349c4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XG58", "query_cov": 1.0, "subject_cov": 0.9778325123152709, "identity": 0.997, "score": 772.0, "evalue": 1.16e-281, "valid": true, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A449AYT1" }, "pscc": { "uniref50_id": "UniRef50_A0A449AYT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1" ], "product": "ABC-type maltose/maltodextrin transporter ATP-binding protein MalK" }, "genes": [], "id": "IGIGPGHMMC_1211", "locus": "GCA000148625_01199" }, { "type": "cds", "sequence": "contig_1", "start": 682400, "stop": 682885, "strand": "-", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG59", "UniRef:UniRef90_C4XG59" ], "nt": "TTGATTGTGCTTTTCCCTGCGGTTTCACTAATTGTGGCCTCACTAAATGTTAACAACCAAAGGGTTGTAGCCATAACACCTTTTAAATTTGGCTTTGACAACTTTACTTATTTATTTACAAGTGAACGAAGCTATTTTTTAAGATGGTATGGTAATACATTATTGCTAGCATTCTTAACTATGGTTATCTCAACCATTTGTGTTGCTTTGAATGCTTATGCTTATTCAAGATTTAAATTCGCTGGTTCAAAACACTCATTAACTATTATTATGATGTTGCAAATGATTCCTGCCACTTCATCTCTTATTTGTCTTTACATTATTGTGCGGATGGGGAATGATTTAGGAATTAATTCAATATTAATGCTGGTTATTATTTATTCTGGTGGAGCTATATCTTCAAATACCTTTATGCTAAAAAGTTATTTAGATACAGTTTCAAGAGAATTAGATGACTCAGCTAGAGTTGATGGATGTTCTAACTGA", "aa": "MIVLFPAVSLIVASLNVNNQRVVAITPFKFGFDNFTYLFTSERSYFLRWYGNTLLLAFLTMVISTICVALNAYAYSRFKFAGSKHSLTIIMMLQMIPATSSLICLYIIVRMGNDLGINSILMLVIIYSGGAISSNTFMLKSYLDTVSRELDDSARVDGCSN", "aa_hexdigest": "946d57a0b219e8e8867b050a5f5c206a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 682103, "stop": 683077, "observations": { "mutation": [ 682339, 682402, 682891, 682924 ], "stop": [ 682339, 682402, 682891, 682924 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG59", "query_cov": 1.0, "subject_cov": 0.49691358024691357, "identity": 0.9940000000000001, "score": 296.0, "evalue": 4.73e-99, "gene_start": 1, "gene_end": 161, "reference_start": 65, "reference_end": 225, "reference_sequence": "MYDKEGLIMNSENKIYDSIVDNISTKKVINKKVRINYNLSDTKPPTPMEIVWLFFNYLILICWSLIVLFPAVSLIVASLNVNNQRVVAITPFKFGFDNFTYLFTSERSYFLRWYGNTLLLAFLTMVISTICVALNAYAYSRFKFAGSKHSLTIIMMLQMIPATSSLICLYIIVRMGNDLGINSILMLVIIYSGGAISSNTFMLKSYLDTVSRELDDSARVDGCSNWGLFFKILLPVIMPALIMTALWSFLTPFTDVILPKFVLISNEEKTLAVGLDILLNAEEKHKNAGAYAAGSILASLPAFCLFMYLQKYIVGGLSEGAVKG" }, "paralog": false, "identity": 0.9814814814814815, "score": 613.0, "evalue": 2.4e-226, "description": "Internal stop codon at 682339, 682402, 682891, 682924. Nonsense mutation around 682339, 682402, 682891, 682924.", "psc": { "uniref90_id": "UniRef90_C4XG59", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XG59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG59", "UniRef:UniRef50_C4XG59" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG59" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "IGIGPGHMMC_1212", "locus": "GCA000148625_01200" }, { "type": "cds", "sequence": "contig_1", "start": 682922, "stop": 683053, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGTGAAAACAAAATTTATGATTCAATTGTTGATAACATTTCAACTAAAAAAGTTATCAACAAAAAAGTTCGTATCAACTATAACTTATCAGATACTAAACCTCCAACACCAATGGAAATAGTTTGA", "aa": "MNSENKIYDSIVDNISTKKVINKKVRINYNLSDTKPPTPMEIV", "aa_hexdigest": "6aaebf92ca75576726d99dd6b9b01785", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4951.672700000002, "isoelectric_point": 9.15762119293213 }, "id": "IGIGPGHMMC_1213", "locus": "GCA000148625_01201" }, { "type": "cds", "sequence": "contig_1", "start": 683056, "stop": 683451, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter permease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "nt": "ATGTTAGTTACTGGTAACTTACAAGCAATTCCAAAAGATATTTATGAAGCTGCTTCTATTGATGGAGCCAAAAATTATCATAAATTCTTTAAGCTAACACTACCTTCTTTATTATTAGCAATTGCCCCAATGTTAATTGGTCAATTTGTTGGAGCATTCAACAACTTCACAACTATTAGTATCTTTAGTGGCGGAGGCCCTGCGTTTGCTAAACCAACTGCTTATCAAGAAGGTGCAACTGATATTCTAATTTCATTTATCTATAAACTAGCCACTGGTGCAGTAAAAATTGAATCTGATCAAGCTTTTGCAGCAGCTTTGACAACTCTTGCAGCTCTATTAAGTATCGCTGTAGCAGCTAGAGGATTCATTAAATCTATGACAAGGAGGGACTAA", "aa": "MLVTGNLQAIPKDIYEAASIDGAKNYHKFFKLTLPSLLLAIAPMLIGQFVGAFNNFTTISIFSGGGPAFAKPTAYQEGATDILISFIYKLATGAVKIESDQAFAAALTTLAALLSIAVAARGFIKSMTRRD", "aa_hexdigest": "7bf93e1623a5a2fd25a7600fba67b3ab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF2AB6", "query_cov": 0.916030534351145, "subject_cov": 0.8633093525179856, "identity": 0.725, "score": 169.0, "evalue": 5.23e-52, "valid": false, "product": "ABC transporter permease subunit", "uniref50_id": "UniRef50_UPI0021AF2AB6" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF2AB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "product": "ABC transporter permease subunit" }, "genes": [], "id": "IGIGPGHMMC_1214", "locus": "GCA000148625_01202" }, { "type": "cds", "sequence": "contig_1", "start": 683848, "stop": 683946, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTATTGTTCTAGCTCCTATTATTACTTCAGTTCTAATTTCATTTACTGACTATGGATACATGCATCAAGCCCCTACTCAACCAGTTCACTGA", "aa": "MLFIVLAPIITSVLISFTDYGYMHQAPTQPVH", "aa_hexdigest": "a3d2a20bfbe018c6d222e1a46d686ce0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3604.2409000000007, "isoelectric_point": 5.927079200744628 }, "id": "IGIGPGHMMC_1215", "locus": "GCA000148625_01203" }, { "type": "cds", "sequence": "contig_1", "start": 684016, "stop": 684336, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTCTTCAAACAAAAAACTAAAGGGATTCTTTTACTTTTAGTAACCACATTATTGTATGCAATTTTTATTCCTTTTAGTTTCGGAGCTTATACTACTGGTATTGATGGTATGGAAGGGATTCTGAGTTTCATTGACTTAGGAGCTCGACACTTCAATTCTAGCATGGGCATATTTAGAGATGCTCGGCGTTACCTTTTTGGTGGTGTCATATCTGTTATTATCTTAACAATAGTATTAATTTACTTTACAGTTTGTTCAATTATTGCATTTAGAACAGCTAAATTAATGGAAGAAGGGTCACGTCCAAGTAAATGA", "aa": "MIFFKQKTKGILLLLVTTLLYAIFIPFSFGAYTTGIDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFTVCSIIAFRTAKLMEEGSRPSK", "aa_hexdigest": "85875a450262fe94b1b9272598bd0912", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11855.068399999987, "isoelectric_point": 9.726554679870606 }, "id": "IGIGPGHMMC_1216", "locus": "GCA000148625_01204" }, { "type": "cds", "sequence": "contig_1", "start": 684487, "stop": 684951, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86", "UniRef:UniRef90_UPI002114FE86" ], "nt": "TTGCTTAAATCTGAACTTTATTTCAAAGAATCGGCAATTTATGAAAAATATTCTTATGAAGGAAATTATAAAAAAGAATTAGCTTTAGCCTTAAAAGAAAGAGCAATAAATGCTGAGCAAGTAAGACTAAAATTCTTATATGAAAAAGTTTTAGCTATTTATGAAACAAAACTAAATTCTTTAAATCTTAGCTCAAATGAAAATTCTAACATTTTAAAAGAACAAGTTAGAAACAAAGAACAATATCAAAGCGAAAAAGAATTAGTTTCTAACAAGAAAAAACAACTTTATAATCAATATCTTGAAACAGTTAAGCAAACAGCTTTAAGATATAAAAATAAAGAAATATCAAAACAAGCTTTTAAACATAGTAAAATGGAAGCAAAGATTGATTATAATGAAAAAAGATATGAATTAAAACTTCAAACTAACAGTCTTAAAAATAAAGAAATTTTATCAACTTGA", "aa": "MLKSELYFKESAIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKVLAIYETKLNSLNLSSNENSNILKEQVRNKEQYQSEKELVSNKKKQLYNQYLETVKQTALRYKNKEISKQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILST", "aa_hexdigest": "5f33af9db1673729df1141a3ff681cf8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 684184, "stop": 684951, "observations": { "mutation": [ 684381, 684489 ], "stop": [ 684381, 684489 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002114FE86", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.948, "score": 263.0, "evalue": 2.59e-85, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.9372549019607843, "score": 463.0, "evalue": 2.65e-166, "description": "Internal stop codon at 684381, 684489. Nonsense mutation around 684381, 684489.", "psc": { "uniref90_id": "UniRef90_UPI002114FE86", "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002114FE86", "UniRef:UniRef50_UPI002114FE86" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18426.88150000001, "isoelectric_point": 9.476417350769044 }, "id": "IGIGPGHMMC_1217", "locus": "GCA000148625_01205" }, { "type": "cds", "sequence": "contig_1", "start": 684952, "stop": 686028, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0", "UniRef:UniRef50_UPI002113FE78", "UniRef:UniRef90_A0A3P8K8F0" ], "nt": "ATGAAGATTAACAAAAATTTATTTTTACGAGCTAGAACAAAACTACAAGATAACTTAAAAAGAGAACTTTCAAGTTTATATGCGGCTTATTCAAATAAAATTAAAGCTATTAAAGATGCTATTAAAAAGATATCGTTTGCTGATAACGAAATCTCAATGATTAAGTATGAAATTAAAGCGTTAACAAAAGAAAAAAAGGCTCTTAAAAAATATGTACTAGAATTTCAAAAATCACTAAGACTAACAGCTGACATTGATGAAAAGAAGAATGAATTATTAGAAGAATTAAAGCAAAAAACAATTAAAGAAGAAAATAAAATTCTTTCTAAATATGCTTTATTCAATATAACTCTAAAATATCTTAAACATAATTCAGATCTTGATTTTGACATCAATAAAATTAAAAACTATTTGCATAAACAAGAACTAAAAATATTAAATACTTTAGAAGACCCAAAATCTTATTTCCAAAACTTTTATCAAAAATTGGAAAAACGTCGTTTAGAATTAATTGAAAAACGTAATTCACTAAACCACAAATATCAAAATAATAAATTTATTGAATTAAAGATTTATAAAGCAAACAAATACAACATTAAATTAGAAACAAATCAAAAAAATCTTGCTTTAGAATATGAATATAATCAAAAAGCAGAATTACAAAAACAAGAAGTAAAAGCATATAAAAAAGAAGCATATGCTAAAATCGAAGAACACAAAAATGAGATTAAACGTGTTGAAAAAGGCAATATTGAAAAAATTAAAAAAATAAAGCAAAATGGAAAATCAAAAATTAAGATTGTAAATCAAAATTTCAAGCAACAACTAAAGAAAATCGATGATTTAGTTGCTACTAGAAATTACCAACAATATTTAGAATTTTTAGCTAAAAATAACTTTATAGATTCTAATATAGATGAATCTATAAAAATTACCAAAAAATCTGTTTTACAGTCATTTAAGAAAAATAAACAACTTGTCTATAATGACAAAAAAACATCTGCTTTAGCTAAAATATTTAAAAAATTATTCTTTGGTTTTTTTAACACAAAAAGCCTAAAAAAAGAATTTGAATGA", "aa": "MKINKNLFLRARTKLQDNLKRELSSLYAAYSNKIKAIKDAIKKISFADNEISMIKYEIKALTKEKKALKKYVLEFQKSLRLTADIDEKKNELLEELKQKTIKEENKILSKYALFNITLKYLKHNSDLDFDINKIKNYLHKQELKILNTLEDPKSYFQNFYQKLEKRRLELIEKRNSLNHKYQNNKFIELKIYKANKYNIKLETNQKNLALEYEYNQKAELQKQEVKAYKKEAYAKIEEHKNEIKRVEKGNIEKIKKIKQNGKSKIKIVNQNFKQQLKKIDDLVATRNYQQYLEFLAKNNFIDSNIDESIKITKKSVLQSFKKNKQLVYNDKKTSALAKIFKKLFFGFFNTKSLKKEFE", "aa_hexdigest": "ef81f8b1995d6d1835fad9b2cfe96b02", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002113FE78", "query_cov": 0.8715083798882681, "subject_cov": 0.8167539267015707, "identity": 0.84, "score": 418.0, "evalue": 1.06e-142, "valid": false, "uniref50_id": "UniRef50_UPI002113FE78" }, "pscc": { "uniref50_id": "UniRef50_UPI002113FE78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002113FE78" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 684649, "stop": 686166, "observations": { "insertion": [ 685911 ], "deletion": [ 685823 ], "mutation": [ 684953, 686142 ], "stop": [ 684953, 685847, 685871, 686142 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F0", "query_cov": 0.9972067039106145, "subject_cov": 0.4518987341772152, "identity": 0.8540000000000001, "score": 493.0, "evalue": 1.07e-166, "gene_start": 2, "gene_end": 358, "reference_start": 48, "reference_end": 404, "reference_sequence": "MEKLKLFNWYGEEFDTILPEEQDTLKAYKHHVRNVVNRRIDKINSQKKINKNLFLRARTKLQDNLKRELSSLYASYSNKIKAIKDAIKKISFANSTISLIKYEIKALIKEKKALKKYVLEFQKSLRLTADTDEKKTELLEELKQKTIKEENEILSKYALFNITLKYLKHNPDLDFDIDKIKNHLHEQELKVLNTLEDPKSYFQNFYQKLENRRLKLIEKRNSLNHKYQNNKSIELKIYKANKYNIKLETNQKILALEYKYNHKAELQKQEVKAYKKEAYAKIEEHKNKIKRVEKDNIEKIKKIKQNGNSKIKIINQNFRQQLKKIDDLVATRNYQQYLEFLAKNNFINSNIEESKKITKKSVLQSFKKSGQLVYNDKKTSALAKIFKKLFFGFFNTKSLKKEFEWLLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.8871287128712871, "score": 799.0, "evalue": 3.79e-289, "description": "Internal stop codon at 684953, 685847, 685871, 686142. Frameshift due to insertion around 685911. Frameshift due to deletion around 685823. Nonsense mutation around 684953, 686142.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F0", "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F0", "UniRef:UniRef50_A0A3P8K8F0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 42890.4982, "isoelectric_point": 9.811266136169433 }, "id": "IGIGPGHMMC_1218", "locus": "GCA000148625_01206" }, { "type": "cds", "sequence": "contig_1", "start": 686571, "stop": 686675, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAATCCATACATTAAACCCAAATTAAATATAGACAAATTTAGTTTTTACTTTTTAGTTTCTTGTTTAGCAGCAATTACAGTCTTAATGATTTTTTGA", "aa": "MDQNPYIKPKLNIDKFSFYFLVSCLAAITVLMIF", "aa_hexdigest": "a0d5561bfb9bc7bbbb48c77e6151620b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3984.785399999999, "isoelectric_point": 7.898231315612794 }, "id": "IGIGPGHMMC_1219", "locus": "GCA000148625_01207" }, { "type": "cds", "sequence": "contig_1", "start": 686675, "stop": 686764, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAAAAATTAACAGCAATTTACAAAGAAAAAATTGACACAATTAATAGTTCAACTGAATCGACAGCTGTTATTAGATTTGAATAA", "aa": "MYEKLTAIYKEKIDTINSSTESTAVIRFE", "aa_hexdigest": "0dc7400cd9bf7eca0ac9adff960cfd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3381.8024000000005, "isoelectric_point": 5.155035209655761 }, "id": "IGIGPGHMMC_1220", "locus": "GCA000148625_01208" }, { "type": "cds", "sequence": "contig_1", "start": 686855, "stop": 687235, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTAATCAATGCAAAAATTGCTGAAGCTAAAAAAGTTTCACAACTTTTATCTAAAAAAGCAGTAGCTGATGAAGTTATTACAAAAGCAGTTAAAGCATATCTTTACGCAAGATACATTAACCAAGCTGAAGTTAGAAATCTTGGTGACAAATTAACTAAAGAATTTCAATTTCCAGCAAAAGATAAAAGCACTAAAGTGCCAGCTTCTAAAGTTATTGAAATTGTTGAACAATATAGAAAAAATCAAACAGTTGACTTAATTTATCAAACATTATCTTCAACTAAATCATTAGCTGATGGTGGATTGAATGTAATTCAATACCAACCAGGACGTATTGACCAATCAAATCCTCAATTTGGTAATGTTGCATGA", "aa": "MQDLINAKIAEAKKVSQLLSKKAVADEVITKAVKAYLYARYINQAEVRNLGDKLTKEFQFPAKDKSTKVPASKVIEIVEQYRKNQTVDLIYQTLSSTKSLADGGLNVIQYQPGRIDQSNPQFGNVA", "aa_hexdigest": "040187f514c64e9f0fa1172cff937f15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14046.941599999984, "isoelectric_point": 9.49382381439209 }, "id": "IGIGPGHMMC_1221", "locus": "GCA000148625_01209" }, { "type": "cds", "sequence": "contig_1", "start": 687422, "stop": 687607, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCAGCTAACAATTTACCAGTACATGCTGTGTTAAACAGTACTTTAGAATCTAAATTAGGCATGAATTTCAAAGATATTCGTGATAAATATACTGAAAAAGGTACTGGATTACGTAATGCATTAGCAGCAATTTTAGGTTTGGAAAAACTTTCGGATTTACAAGGTAATGGCCAAACTTGA", "aa": "MYSANNLPVHAVLNSTLESKLGMNFKDIRDKYTEKGTGLRNALAAILGLEKLSDLQGNGQT", "aa_hexdigest": "9e1b85fcaf7c5daa9a61b449701ccc49", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6621.468400000001, "isoelectric_point": 8.08609218597412 }, "id": "IGIGPGHMMC_1222", "locus": "GCA000148625_01210" }, { "type": "cds", "sequence": "contig_1", "start": 687647, "stop": 687760, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAATTGAAATATACAGATGCTGCTGAATTTAATAGAGATTATGATTCATCAATTCAAGTAATTAATAATTTATCAAATGTAGGCGATTCTCCAAGTGCAAGTACTTGA", "aa": "MSKLKYTDAAEFNRDYDSSIQVINNLSNVGDSPSAST", "aa_hexdigest": "cd325d09b4557dbba019b2b12b6d5bc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4039.3065000000006, "isoelectric_point": 4.359459877014161 }, "id": "IGIGPGHMMC_1223", "locus": "GCA000148625_01211" }, { "type": "cds", "sequence": "contig_1", "start": 688165, "stop": 688368, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAAATTACTTTTCTTAAAAAATGGAAAAATTTCAACAGGTCTTTACACAGAAAATCCTATCCATGTTGAATTCAAAAAAGCAGTTGATTTAATGTCAGAATTCTTCTTTCCTATATATGAAGCTGCATATATCCTAGATCAGGAGCATATGCTACTTCAGTATGAGGCAAAAGAAATATTAAACAAGATGACTTAA", "aa": "MMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDQEHMLLQYEAKEILNKMT", "aa_hexdigest": "2bd9afbd28508ef7df212752ac19c26e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7947.332399999996, "isoelectric_point": 6.056615257263183 }, "id": "IGIGPGHMMC_1224", "locus": "GCA000148625_01212" }, { "type": "cds", "sequence": "contig_1", "start": 688420, "stop": 688806, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26", "UniRef:UniRef90_UPI0001E32F26" ], "nt": "ATGCCTACAGGGGCAACAGATGAAAAAATTGTGCCAGATATTTTTTATGCACCACATGATAGAATTACAACTTTAGTTGCATCTAATGCAGTTGCTGATTTAGATCAATTTTCACCAGGATTATTAGATCGTATTGCTAAAAAAGTTAAAGCAACTGAAGAAGAAAAGAAACAACTTAGATCATTTGGTTCAGTAGTCGGTATTGAAAAAATTACTCAATTACCAGCTGAAAAACTTTTTGGTATTAGACACAACACTGAAGGTGTTATAGTTGCTTCAACAAAATCTTTAGCAGATGTTAAAAAAGACTTAGCTAAACCTGAAAATGATACATTACTAAAATTAGTAAAACAAGGTCGTGCATTTTTAAGATTCCAAGACTTCTGA", "aa": "MPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDF", "aa_hexdigest": "e3df7699676ab7edcd1fc9399818730e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 688165, "stop": 689049, "observations": { "mutation": [ 688422, 688821 ], "stop": [ 688422, 688821 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32F26", "query_cov": 1.0, "subject_cov": 0.43537414965986393, "identity": 1.0, "score": 248.0, "evalue": 8.98e-81, "gene_start": 1, "gene_end": 128, "reference_start": 82, "reference_end": 209, "reference_sequence": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDVWGALEMPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDFWYGNGILGAVAHEYKAEDMMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDQEHMLLQYEAKEILNKMT" }, "paralog": false, "identity": 0.9931972789115646, "score": 566.0, "evalue": 8.23e-209, "description": "Internal stop codon at 688422, 688821. Nonsense mutation around 688422, 688821.", "psc": { "uniref90_id": "UniRef90_UPI0001E32F26", "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32F26", "UniRef:UniRef50_UPI0001E32F26" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14150.149499999978, "isoelectric_point": 8.859583854675293 }, "id": "IGIGPGHMMC_1225", "locus": "GCA000148625_01213" }, { "type": "cds", "sequence": "contig_1", "start": 688819, "stop": 689049, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAATTTTAAGAAAACTTTTAAAAAAATAGCAATTGGCTTTGCAACCTTAGCAGCTCCAGTGACAGCATTAGCTACAATTAGTTGTGGTACTACTGAAAGCAAAACAATTTTAATTGCAGTTGATGGCGTTCAACAAAAATTCTATGACAAAGCAATTGAATTGTTTAAGAAAACAAAATCAGGCCAAAATGGCTATGAAATTAAAACAATAGCTAAAGATGTTTGA", "aa": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDV", "aa_hexdigest": "05fdf836fc0fafb13ac8783bb023d959", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8232.566499999997, "isoelectric_point": 9.630819129943848 }, "id": "IGIGPGHMMC_1226", "locus": "GCA000148625_01214" }, { "type": "cds", "sequence": "contig_1", "start": 689893, "stop": 690060, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F28", "UniRef:UniRef90_UPI0001E32F28" ], "nt": "ATGTTTGCTGAAAAAGAAAATACTTATGCTAATTTAGATGATCTCTTTAAACCCGGTTATGATTATAAAGGACTTGGATATAAATCAGTTAAAGCAAAGCAAAAAGATAAAAAGTTTTTAGTTTTAGTCAATTCAATTTATTATCATACAGCTTATGGTCAAGAATGA", "aa": "MFAEKENTYANLDDLFKPGYDYKGLGYKSVKAKQKDKKFLVLVNSIYYHTAYGQE", "aa_hexdigest": "1d9804149a3fc57f8d979d32e93d8dd2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 689767, "stop": 690060, "observations": { "mutation": [ 689895 ], "stop": [ 689895 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32F28", "query_cov": 1.0, "subject_cov": 0.5670103092783505, "identity": 1.0, "score": 117.0, "evalue": 3.37e-33, "gene_start": 1, "gene_end": 55, "reference_start": 1, "reference_end": 55, "reference_sequence": "MFAEKENTYANLDDLFKPGYDYKGLGYKSVKAKQKDKKFLVLVNSIYYHTAYGQEWNLVFKNAPTKINVIFSNNSSINNEKITFSGNTGLMLVELTY" }, "paralog": false, "identity": 0.9896907216494846, "score": 195.0, "evalue": 2.61e-66, "description": "Internal stop codon at 689895. Nonsense mutation around 689895.", "psc": { "uniref90_id": "UniRef90_UPI0001E32F28", "uniref50_id": "UniRef50_UPI0001E32F28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32F28", "UniRef:UniRef50_UPI0001E32F28" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F28" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6432.225900000003, "isoelectric_point": 8.831346702575683 }, "id": "IGIGPGHMMC_1227", "locus": "GCA000148625_01215" }, { "type": "cds", "sequence": "contig_1", "start": 690233, "stop": 690427, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGGAGTTAAAACTTTTTATCTCGAAATTAAGCAAAATGATTTTAAAGTTTTAAATGTAATGAGTTATGGATTAAGTAACCTATTAATTCAAAGTGGTAATCCAATTCTTTATAATGGTAATGAATTAAATATGAGAGGTGGCCCACAAGCACATTCTGATGCTTTTTTAAGAGAAGCTTTTAAATGA", "aa": "MIFGVKTFYLEIKQNDFKVLNVMSYGLSNLLIQSGNPILYNGNELNMRGGPQAHSDAFLREAFK", "aa_hexdigest": "02683061485069b064bc45f3d1003325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7254.2836, "isoelectric_point": 8.085898780822756 }, "id": "IGIGPGHMMC_1228", "locus": "GCA000148625_01216" }, { "type": "cds", "sequence": "contig_1", "start": 690683, "stop": 690832, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAGAAAATAAACATGAATTTAAAGACGCAACAGGCTTAGAAAAGAGAAAATTATTTAATGAGCAAAGATTAGCCTTAAGAGAAGTAATAAAAGAAACTAAAGATCAAAGATCAAGCGATGATATATTTTTCTTTGGTGAATGA", "aa": "MNSENKHEFKDATGLEKRKLFNEQRLALREVIKETKDQRSSDDIFFFGE", "aa_hexdigest": "58a91c581e03181e6b7760ec7eedf752", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.4847, "isoelectric_point": 5.796065330505371 }, "id": "IGIGPGHMMC_1229", "locus": "GCA000148625_01217" }, { "type": "cds", "sequence": "contig_1", "start": 691160, "stop": 691498, "strand": "-", "frame": 1, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "nt": "TTGTCTTATGAAGGTGAATTTAAAAATGCAAAATTTATTGTGCCTTTTAACAAATCTATTAAGCCTTCAAATGTTATGTATCAATTAACAATTTATTCTTTTGCAGATGGCAATAATGATGGTATTGGCGACTTTATTGGACTAAATGAAAATTTAGAATATTTTAGCAATTTAGGAATTGATACTTTATATCTTTCACCAATACATCCAGCCAGCTCATATCATGGCTATGATGTAATTGATTATACAGATGTTGCTCCTGAATTAGGTGGCATGGAAGCCTTTGATCAATTTTTAATAAATGCCCATAAAAAAGGCATAAAAGTTATTATGGATTGA", "aa": "MSYEGEFKNAKFIVPFNKSIKPSNVMYQLTIYSFADGNNDGIGDFIGLNENLEYFSNLGIDTLYLSPIHPASSYHGYDVIDYTDVAPELGGMEAFDQFLINAHKKGIKVIMD", "aa_hexdigest": "92899d05716cb1b7e7f0ea738b36f460", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF384D3", "query_cov": 0.9285714285714286, "subject_cov": 0.832, "identity": 0.779, "score": 172.0, "evalue": 9.51e-54, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI002FF384D3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF384D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "IGIGPGHMMC_1230", "locus": "GCA000148625_01218" }, { "type": "cds", "sequence": "contig_1", "start": 691540, "stop": 691641, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3", "UniRef:UniRef50_A0AB32XCK3", "UniRef:UniRef90_A0AB32XCK3" ], "nt": "ATGAGTAAAAAAACAAGAAAAAAATTAGCATATTTTTCTTTAATTGCACCTATCGCTTTATGTTTAAGTGCAAGTTGCACAAGTGTTAAAAAAGATTATTAA", "aa": "MSKKTRKKLAYFSLIAPIALCLSASCTSVKKDY", "aa_hexdigest": "0d0991061a8fb19f63031ca6f39809b5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59D51", "uniref100_id": "UniRef100_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCK3", "uniref90_id": "UniRef90_A0AB32XCK3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCK3", "uniref50_id": "UniRef50_A0AB32XCK3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCK3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3666.4218999999994, "isoelectric_point": 9.835119438171386 }, "id": "IGIGPGHMMC_1231", "locus": "GCA000148625_01219" }, { "type": "cds", "sequence": "contig_1", "start": 692812, "stop": 693369, "strand": "-", "frame": 2, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5", "UniRef:UniRef90_C4XG67" ], "nt": "ATGCAAAAAAATGAGAATTTTACCAATAATATTGCAGCCAATGAAGATGACAAATCTTTTAATGAATTATCATTTGGCAAAAAGTTTAAGCAACACTTCGCTAAGTCATTTCGTCTTAGTGTTTTAGATATTGCTTTAGCAGGTATATTATTGGCAATTCATTTTGTTGTTGCAATAATTTCTAAGTTTACAATTTTAAAAATAATACCCTTAGAAACTGATGCAATTTTTTTTATATTATATGGAATCTTTTTTGGACCACTTAAAGGATCTTTAATATCATTTTTAGCTGATACTTTTATGATGCTTTTAACTGGTACAATTGGAACTTGGTATTATCTTTATGCATTGATTCCAATAGGCATTTGTATTTTAAGCTCCTTTTATTACTTTTTTTATCGTGCTAGTTTTTTATTTAGAATTATTTTTCCAATAATTGTAATTTCAGTTGGTTTCGCTTTATTACTCTATGTTGTTATAAATAGTGGAAATGATAAAGGTTATCATATTAAAGGAATTAAGAAGCTAAAATATTTACCTAAAAATGCAGTTTTATGA", "aa": "MQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVL", "aa_hexdigest": "a94329f6d0f86e7ade84262bce6e0fec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 692509, "stop": 693381, "observations": { "mutation": [ 692661, 692718, 692814 ], "stop": [ 692661, 692718, 692814 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG67", "query_cov": 1.0, "subject_cov": 0.589171974522293, "identity": 1.0, "score": 343.0, "evalue": 2.58e-117, "gene_start": 1, "gene_end": 185, "reference_start": 5, "reference_end": 189, "reference_sequence": "MIQNMQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVLWMLLIYFNFSVIAFIFIIFQLLFAKNKKVKKRWFDYLFVFSIIIFIMIIFRWLIGPYVYINYYNYLYAGRKSLNYTGEHYYLITIPILLKSLITIPIYSIIMNPIFSVAILLKEKFVEKNSKISY" }, "paralog": false, "identity": 0.9827586206896551, "score": 533.0, "evalue": 1.42e-194, "description": "Internal stop codon at 692661, 692718, 692814. Nonsense mutation around 692661, 692718, 692814.", "psc": { "uniref90_id": "UniRef90_C4XG67", "product": "ECF transporter S component", "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG67", "UniRef:UniRef50_A0A3P8LHP5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5" ], "product": "G-protein coupled receptors family 1 profile domain-containing protein" } }, "id": "IGIGPGHMMC_1232", "locus": "GCA000148625_01220" }, { "type": "cds", "sequence": "contig_1", "start": 693673, "stop": 694461, "strand": "+", "frame": 1, "gene": null, "product": "Phosphomannomutase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68", "UniRef:UniRef90_C4XG68" ], "nt": "TTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGA", "aa": "MTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFK", "aa_hexdigest": "b9ad7efd49076ab2d92f7ab18b067983", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 693373, "stop": 694764, "observations": { "insertion": [ 693439 ], "deletion": [ 693467 ], "mutation": [ 694460 ], "stop": [ 694460 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG68", "query_cov": 1.0, "subject_cov": 0.4720720720720721, "identity": 0.996, "score": 528.0, "evalue": 4.76e-185, "gene_start": 1, "gene_end": 262, "reference_start": 123, "reference_end": 384, "reference_sequence": "MKRFVIFIGCNIIGKILIFLHILNYMLKIFICYYIIYIMNKKLEFGTAGIRGILGSGEDHLNVAHVQRVIHGFAKYLKNKYSHINPITVVIGRDNRRFSATFATLSAQILSQYDIKVIFPLYLTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFKWIGMIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDSWLALRPSGTEPKIKFYIFAFGKDQQEAQAKFDNFARIIKEIK" }, "paralog": false, "identity": 0.9913606911447084, "score": 907.0, "evalue": 0.0, "description": "Internal stop codon at 694460. Frameshift due to insertion around 693439. Frameshift due to deletion around 693467. Nonsense mutation around 694460.", "psc": { "uniref90_id": "UniRef90_C4XG68", "product": "Phosphomannomutase", "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG68", "UniRef:UniRef50_C4XG68" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68" ], "product": "Phosphomannomutase" } }, "id": "IGIGPGHMMC_1233", "locus": "GCA000148625_01221" }, { "type": "cds", "sequence": "contig_1", "start": 694468, "stop": 694848, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGATGATTCTTGA", "aa": "MIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDS", "aa_hexdigest": "1f68d738d7186442c746fb060d112ead", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 57, "aa_cov": 0.4523809523809524, "hmm_cov": 0.9649122807017544, "evalue": 1.0352507749184268e-06, "score": 24.884675979614258, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14355.937999999982, "isoelectric_point": 4.472285270690917 }, "id": "IGIGPGHMMC_1234", "locus": "GCA000148625_01222" }, { "type": "cds", "sequence": "contig_1", "start": 695042, "stop": 695236, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAA", "aa": "MNFLKSPLIDYFSSTYLVIKLPEIPITGLTEIHNLVSKVFKSRIMLRVSKRSIVVTTLYVFKPE", "aa_hexdigest": "67dff05b1e79d9cedba223846d52d62f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7415.8406, "isoelectric_point": 9.89874973297119 }, "id": "IGIGPGHMMC_1235", "locus": "GCA000148625_01223" }, { "type": "cds", "sequence": "contig_1", "start": 695269, "stop": 695541, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATAACATTCTTAATCATTTTTGGTTTTGCTCAACCACATCATAACTATGCAAAAATTTTAACAAATGGTGTTGGAACTCAAATTATTGAAGATTCAGGTAACAGAGTATTCTTCGGAATGAGAGAATTTACAACATTCTTCTATATATTGGTTGTTAACTTCTTAATTAACTTTATTTATCCTAAATATAAAAAAGTTGCAGTTGAAATTTCTTGTGGACCTTCACCAGATTTAGTGTTAGCGTACTTTAAGAAAATTAACTATTGA", "aa": "MTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINY", "aa_hexdigest": "1ec7a4f6836d5d243dfd1b43fbe04920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10396.10749999999, "isoelectric_point": 9.13157596588135 }, "id": "IGIGPGHMMC_1236", "locus": "GCA000148625_01224" }, { "type": "cds", "sequence": "contig_1", "start": 695791, "stop": 696342, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69", "UniRef:UniRef90_C4XG69" ], "nt": "ATGAATAACAACGAAAAAGATACTACCAAACTAGAACAAGTGCATAACCTTGAAGTTAGTAAAGAAGCTATTCAAGAAGTTAAAGATATGGTTTTTGAAGCAAAAAATCAAATCCAAGAACCTAAAAAGGAAATTCTTGATGAAAAATCTCTTGACAGTCTTGAAATGGTCCGTCATAATTATGAAAAAGATAATCCTAAAAACAAGGAAGTTAGGAAAAGAATTGCTAAACTCTATGCTCGTCGTTTAGTTTTTATCTTTTTATCAGCATTGGTCTTTAACTTTGGTGTGCAAGTATTCTTAAATAGAGGTGAAACTATTCCTAGTGGACTTAGTGGAGCTCCTTTATTAATTAACTTAATTGCACCGAGTACTAAGCCTTATTTTGCTTTAATTTACTTAGCTTTTAACTTACCGCTTCTTTTAGGATTTGGATTTAAGATTAAGAAAAGTTTTACTTTGATGACACTAGCTTTTATGCTATTTCAAATATTAACTAATTTGATATTAACTTCAGTTCCAATTGGAGAAAATAAAATAGTTTTAGACTGA", "aa": "MNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLD", "aa_hexdigest": "3c08ae7db96ca4ca29606584b3feb53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 695488, "stop": 696351, "observations": { "mutation": [ 695646, 695709, 695760, 695793 ], "stop": [ 695646, 695709, 695760, 695793 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG69", "query_cov": 1.0, "subject_cov": 0.4295774647887324, "identity": 1.0, "score": 321.0, "evalue": 8.24e-107, "gene_start": 1, "gene_end": 183, "reference_start": 4, "reference_end": 186, "reference_sequence": "MNIMNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLDWLHEHINFAPGWTKEIKVGSVTLENPNSWPIFAYGAIGSGCAGTAIAIAWKNGGSTGGTDIVAYYFSTKKQKSVGNILSLISIIMTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINYWHGYTVVEGISGYSGLKTYSVVTTMLLLETRSIIRDLKTLDTKLWISVKPVIGISGSFITKYVDEK" }, "paralog": false, "identity": 0.9825783972125436, "score": 527.0, "evalue": 2.04e-190, "description": "Internal stop codon at 695646, 695709, 695760, 695793. Nonsense mutation around 695646, 695709, 695760, 695793.", "psc": { "uniref90_id": "UniRef90_C4XG69", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG69", "UniRef:UniRef50_C4XG69" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69" ], "product": "ABC transporter permease" } }, "id": "IGIGPGHMMC_1237", "locus": "GCA000148625_01225" }, { "type": "cds", "sequence": "contig_1", "start": 696487, "stop": 696786, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02888", "RefSeq:WP_013354830.1", "SO:0001217", "UniParc:UPI0001A8C1DC", "UniRef:UniRef100_C4XG70", "UniRef:UniRef50_A9NF54", "UniRef:UniRef90_C4XG70" ], "nt": "ATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGTCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAATCCTAAATCAACTCACAAGAGAAAATTAGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAG", "aa": "MIAIIETGGKQILVKEGQTIFIEKIEGKEGSTVDFDKVLLIDNKIGKPYLKGAKVTGKIQKQGKAKKIVVYRHNPKSTHKRKLGHRQPYTRVEITKIVG", "aa_hexdigest": "912f8497cc2d988986112d18fe7b9be6", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1DC", "ncbi_nrp_id": "WP_013354830.1", "uniref100_id": "UniRef100_C4XG70", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DC", "RefSeq:WP_013354830.1", "UniRef:UniRef100_C4XG70" ] }, "ips": { "uniref100_id": "UniRef100_C4XG70", "uniref90_id": "UniRef90_C4XG70" }, "psc": { "uniref90_id": "UniRef90_C4XG70", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_A9NF54", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A9NF54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NF54" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "IGIGPGHMMC_1238", "locus": "GCA000148625_01226" }, { "type": "cds", "sequence": "contig_1", "start": 696796, "stop": 697194, "strand": "+", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71", "UniRef:UniRef50_A0A3P8MF53", "UniRef:UniRef90_C4XG71" ], "nt": "ATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCAGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAA", "aa": "MAHTKAGGSTRNGRDSRGQRLGIKLGDGQFCTAGSIIFRQRGTKIFPGTNCGRGNDDTLFALITGYVKFETRRNRTYASVYPERVSSALSQPVVKKAAVKKETAPKVAVKKPAAKKATTTTKKVSAAKKATK", "aa_hexdigest": "9de227b38602171f777e5432384a2e2f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C1DD", "uniref100_id": "UniRef100_C4XG71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71" ] }, "ips": { "uniref100_id": "UniRef100_C4XG71", "uniref90_id": "UniRef90_C4XG71" }, "psc": { "uniref90_id": "UniRef90_C4XG71", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_A0A3P8MF53", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF53" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "IGIGPGHMMC_1239", "locus": "GCA000148625_01227" }, { "type": "cds", "sequence": "contig_1", "start": 697373, "stop": 697552, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGA", "aa": "MLFQVISFLNKSTDSSTLKEELVIFEYTSPYKQLIVIPAIVAAAKMIKIVPKTQFFLAI", "aa_hexdigest": "79b1208bcc328e84e83f8063087d7015", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6678.974700000001, "isoelectric_point": 9.158330345153807 }, "id": "IGIGPGHMMC_1240", "locus": "GCA000148625_01228" }, { "type": "cds", "sequence": "contig_1", "start": 697531, "stop": 697737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCACAGTTGCTATTTCAATGAAGGATGGATTTGCTGCTTGTAAAGAATTTATAAATAACATTTTTCATAATTCAACTTCAATTTCATTAATTGGTGGCTCAATTATTGCTTTAGTTGCAATTGCTTTATTCTTTATTCTTTCAACAGCTACAGTCATTGTTTACTTTGTAACATCAATAGCTCATATAGCAAGAAAGAATTGA", "aa": "MCTVAISMKDGFAACKEFINNIFHNSTSISLIGGSIIALVAIALFFILSTATVIVYFVTSIAHIARKN", "aa_hexdigest": "069d942e15ab03247d75e3823e083ee3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7280.594299999996, "isoelectric_point": 8.80420551300049 }, "id": "IGIGPGHMMC_1241", "locus": "GCA000148625_01229" }, { "type": "cds", "sequence": "contig_1", "start": 697741, "stop": 697899, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAGTAGAACTAAATTTATTCCGCTAATAGTATTATTAGCTTTTATAGTTGGCTTTTGTTTATTGTTCATTTTTGGTATGCAACCTGAAATGATCATCAAAGAAGAAGCCAAACCAGCAACAACTGAATTTGGAGTAGGATTCTTTGGCACTTGA", "aa": "MYSRTKFIPLIVLLAFIVGFCLLFIFGMQPEMIIKEEAKPATTEFGVGFFGT", "aa_hexdigest": "bd8d04303f92e7127262776e09805023", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5864.052899999999, "isoelectric_point": 6.047066307067872 }, "id": "IGIGPGHMMC_1242", "locus": "GCA000148625_01230" }, { "type": "cds", "sequence": "contig_1", "start": 698019, "stop": 698225, "strand": "-", "frame": 3, "gene": null, "product": "Alcohol dehydrogenase 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "nt": "TTGTTCGAAAGAGAATTAGCTGGATCAATCGTTGGTACAAGACTTGATTTACAAGAATCACTTCAATATGCTGCAGAAGGAAAAGTTAAATCAGAAGTTACAAAAGTTATTAAACTAGAAGAAGTTTCAGAAGTATTTGAAAAACTTCAAAAAGGTGAATTCTTAGGACGTGCTGTTATTGACTTTGGTTACAGAGGAAAGAAATAA", "aa": "MFERELAGSIVGTRLDLQESLQYAAEGKVKSEVTKVIKLEEVSEVFEKLQKGEFLGRAVIDFGYRGKK", "aa_hexdigest": "1b165d6d82f2a73a84a98f6122b31cfd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P3G6", "query_cov": 0.8823529411764706, "subject_cov": 0.9523809523809523, "identity": 0.8170000000000001, "score": 96.7, "evalue": 3.91e-25, "valid": false, "product": "Alcohol dehydrogenase 1", "uniref50_id": "UniRef50_A0A3B0P3G6" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P3G6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "product": "Alcohol dehydrogenase 1" }, "genes": [], "id": "IGIGPGHMMC_1243", "locus": "GCA000148625_01231" }, { "type": "cds", "sequence": "contig_1", "start": 698232, "stop": 698573, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGTTGTTATCGGTGTTGGTGGACTTGGCCAATTAGCAATTCAATATGCTAAAGCAATGGGATATAGACCTATTGGTATCGACTTAACAGATGAAAAATGCGAATTAGCTTTAAAATCAGGAGCAGAATTTGCATTCAACTCTAAGAAAGATCCAAACTTTATTCAAAAACTTATTGAAAAGACTGGTGGTGGAGCTCACGCTGTAATTAACACATCAGTTGCTACAATTGCTGGTGAACAAGGCATGGAAATGCTTCGTCGTGGAGGACGTCAAGTATTAGTAGGCCTTCCATCAAAAGACAAACTTGGTAAAGACAACATTGGCGTTTCAGTATTCTGA", "aa": "MVVIGVGGLGQLAIQYAKAMGYRPIGIDLTDEKCELALKSGAEFAFNSKKDPNFIQKLIEKTGGGAHAVINTSVATIAGEQGMEMLRRGGRQVLVGLPSKDKLGKDNIGVSVF", "aa_hexdigest": "711b2e3fb273a542520c76c9ad915162", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 95, "aa_cov": 0.831858407079646, "hmm_cov": 0.9894736842105263, "evalue": 8.685327808163238e-26, "score": 86.43392181396484, "start": 8, "stop": 101 } ], "seq_stats": { "molecular_weight": 11905.738499999992, "isoelectric_point": 9.130608940124514 }, "id": "IGIGPGHMMC_1244", "locus": "GCA000148625_01232" }, { "type": "cds", "sequence": "contig_1", "start": 698574, "stop": 698744, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCTTACACATTTGATGGATCATTTGCTGAATATGCAATTGGACACGAAGATTATGTAGGAATTGTTCCAGAAGGATTAGATTTAATTACTGGAGCACCTGTAGTATGTGCTGGTGTTACAACCTATAAGGCAATTAAAAGAGCTAAAGCTCGTCCAGGTTACTGA", "aa": "MSAYTFDGSFAEYAIGHEDYVGIVPEGLDLITGAPVVCAGVTTYKAIKRAKARPGY", "aa_hexdigest": "7bc716c17e1ab59e296874f5d62d1db6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5941.698300000004, "isoelectric_point": 5.563707542419433 }, "id": "IGIGPGHMMC_1245", "locus": "GCA000148625_01233" }, { "type": "cds", "sequence": "contig_1", "start": 699036, "stop": 699140, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATAAAACATTAAAAAATTACTATTATTTTTTTAAGGAGATAGATTTTATGGCAAAAACAACTATGAAAGCTTTTGTTGTTGAAAAACCAAAACAATGA", "aa": "MFYKTLKNYYYFFKEIDFMAKTTMKAFVVEKPKQ", "aa_hexdigest": "d2de4e8ac69d91b8acd5697a4390473f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4280.078300000001, "isoelectric_point": 9.574538230895993 }, "id": "IGIGPGHMMC_1246", "locus": "GCA000148625_01234" }, { "type": "cds", "sequence": "contig_1", "start": 699293, "stop": 700024, "strand": "+", "frame": 2, "gene": null, "product": "DNA processing protein", "db_xrefs": [ "RefSeq:WP_013354834.1", "SO:0001217", "UniParc:UPI0001E32F2F", "UniRef:UniRef100_UPI0001E32F2F", "UniRef:UniRef50_C4XFY3", "UniRef:UniRef90_C4XFY3" ], "nt": "ATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAACAAAAAAATTAATCAAGCAGAAATAGATTCAATACTAAAGGAGTTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACAAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAA", "aa": "MNELLVYLSYKYEGNIFEIYKFLKNNKKINQAEIDSILKELEEKGIKYITALDENFPEILKNFRYSPYVLFYKGNIGLLNKKAVCLTGDIESPIVDINIKKSQNTFLENYPLITNDFKNLDQKFVEIYRQNKKEIIHVLPYGLDYIKETINLDTELYISQYPPQTHAKLFRFKERNILVSFLAEFLIVYSSKSDSGILSLATAFANNNKEVYCYPGLSYDDGNNYLIKLGANLITQIAEVQYY", "aa_hexdigest": "4102c0313ee8b156659bbb7f07ff212c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F2F", "ncbi_nrp_id": "WP_013354834.1", "uniref100_id": "UniRef100_UPI0001E32F2F", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F2F", "RefSeq:WP_013354834.1", "UniRef:UniRef100_UPI0001E32F2F" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F2F", "uniref90_id": "UniRef90_C4XFY3" }, "psc": { "uniref90_id": "UniRef90_C4XFY3", "product": "DNA processing protein", "uniref50_id": "UniRef50_C4XFY3" }, "pscc": { "uniref50_id": "UniRef50_C4XFY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY3" ], "product": "DNA processing protein" }, "genes": [], "id": "IGIGPGHMMC_1247", "locus": "GCA000148625_01235" }, { "type": "cds", "sequence": "contig_1", "start": 700021, "stop": 700161, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAAAAAATGCTCACATTGGTTTTGCAATGGGAAATGCCACTGAAGAAGCAAAAGTAGCTGCCGATAGAGTTATAGGTTCTAATTTAACTGGCGCTATTGGTCAAGCAATTAATGAAATTTTAGACGACAAAAATTAA", "aa": "MLKNAHIGFAMGNATEEAKVAADRVIGSNLTGAIGQAINEILDDKN", "aa_hexdigest": "3a66b63781eb15e149a092db13a4386e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 38, "aa_cov": 0.8260869565217391, "hmm_cov": 1.0, "evalue": 6.7536251062769965e-09, "score": 31.815629959106445, "start": 1, "stop": 38 } ], "seq_stats": { "molecular_weight": 4783.357, "isoelectric_point": 4.950130653381346 }, "id": "IGIGPGHMMC_1248", "locus": "GCA000148625_01236" }, { "type": "cds", "sequence": "contig_1", "start": 700246, "stop": 700380, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTAAGAAATCCATTAATTACTGCAGCAGAAACCACAAAGTATTTTCAAAATAAATTAAAAGATAAACAAAAAGTTTTCTTAACAAACTCAATTTACACTGATGTTAATCCTTTAGGTGTAACTAAGTGA", "aa": "MALRNPLITAAETTKYFQNKLKDKQKVFLTNSIYTDVNPLGVTK", "aa_hexdigest": "abd291e4e154f62124e4ba317619ee8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5014.794700000002, "isoelectric_point": 9.777162361145017 }, "id": "IGIGPGHMMC_1249", "locus": "GCA000148625_01237" }, { "type": "cds", "sequence": "contig_1", "start": 700519, "stop": 700869, "strand": "-", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "EC:3.1.3.104", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_C4XFY4", "UniRef:UniRef90_A0A3P8KLL0" ], "nt": "ATGCAAAACAAAAGAAAAGTGTTTGCTTTTGATTTGGATGGCACATTATTAACAGATGGAGTTAATACTCATCCAGAAACTCAAGAAGCAATTTTAAAATCATATAATTTAGGACATGCGAATATCATTTGTACAGGTCGTGGTTTGCAAAAAACTTTACCACTTTTAAAATCAATTAAAGGTATTAATTATTTTGTATGTTCAAATGGTGCATTATTTTATGATGTTGAAAAAGATTATTATAAAGTTTTAGGCGAAGTTAATAAAGATATATTTAATGATCTTTTTGATTATGCTCAAGCTCATGATTTAATTTTAACTATTGATACTCCTGAATTTAATGGTGCATGA", "aa": "MQNKRKVFAFDLDGTLLTDGVNTHPETQEAILKSYNLGHANIICTGRGLQKTLPLLKSIKGINYFVCSNGALFYDVEKDYYKVLGEVNKDIFNDLFDYAQAHDLILTIDTPEFNGA", "aa_hexdigest": "0a4025e14158ec0fc81e5f56f09c34d2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 700216, "stop": 700869, "observations": { "mutation": [ 700248, 700485, 700521 ], "stop": [ 700248, 700485, 700521 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KLL0", "query_cov": 1.0, "subject_cov": 0.4128113879003559, "identity": 0.845, "score": 207.0, "evalue": 4.37e-65, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MSKKRIVFAFDLDGTLLTDGVNAHPETQDAIIKSYNQGHANIICTGRGLQKTLPLLKTIKGINYFVCSNGALFYDVEKDYYRVLGEVDKSIFNELFDYANTNNLILTIDTPDFNGSWSKNSDKNILPEWVSKQNKLSDKNPHFLKPLSALTSVLNNPESKITQMAIRNPLDSAQETTKHFQGRLKGKQKVFLTNSIYTDVNPLHTTKWYGLKTLLDYLKIDTHELYCFGDSGNDIEMLQNAHIGYAMGNGTNDAKLAADKIIGSNLTGAIGQVINEILEKN" }, "paralog": false, "identity": 0.7695852534562212, "score": 336.0, "evalue": 2.26e-118, "description": "Internal stop codon at 700248, 700485, 700521. Nonsense mutation around 700248, 700485, 700521.", "psc": { "uniref90_id": "UniRef90_A0A3P8KLL0", "product": "COF family HAD hydrolase protein", "ec_ids": [ "3.1.3.104" ], "uniref50_id": "UniRef50_C4XFY4", "kegg_orthology_id": "K21064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KLL0", "EC:3.1.3.104", "UniRef:UniRef50_C4XFY4", "KEGG:K21064" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY4" ], "product": "COF family HAD hydrolase protein" } }, "id": "IGIGPGHMMC_1250", "locus": "GCA000148625_01238" }, { "type": "cds", "sequence": "contig_1", "start": 701187, "stop": 701561, "strand": "-", "frame": 3, "gene": "ecfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "db_xrefs": [ "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_C4XFY5", "UniRef:UniRef90_C4XFY5" ], "nt": "ATGATTATGGTTACTTCTTTATTATTGATTACAACCAAACCAATATTACTTACTAAAGCACTTGAAGACTTGATGTTTCCATTTAAACTTCTATTCATTCCAACTCACATAATTGCTATGATTATTTCGATTGCGTTAAGATTTATTCCTACTCTTTTTGAAGAAGCACAAAGAATAATGAAAGCTCAAGCAAGTCGTGGAGTTGACTTTAAGAATGGAAAAATTAAAGAAAAAGTTAAATCATTTACAACATTAATTATTCCATTGTTTGTTACTTCTTTTGCTAAAGCGGAAGACTTATCAAATGCTATGGAAACTAGGGGTTATGATCCTTATGCAAAAAGAACAAGATATCGTAAATTAAGAATAGGTTGA", "aa": "MIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIG", "aa_hexdigest": "569452122d6e589593b0f7f8381d720c", "start_type": "ATG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 701070, "stop": 701861, "observations": { "mutation": [ 701099, 701189, 701639 ], "stop": [ 701099, 701189, 701639 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY5", "query_cov": 1.0, "subject_cov": 0.4290657439446367, "identity": 1.0, "score": 228.0, "evalue": 2.51e-73, "gene_start": 1, "gene_end": 124, "reference_start": 127, "reference_end": 250, "reference_sequence": "MMKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIPWGGHSEYALTLEALARTGALIIRIYIMIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIGWLDILFFILIVGLILFVAFSQSAKYDFLPFWYKFTYFKY" }, "paralog": false, "identity": 0.9847908745247148, "score": 494.0, "evalue": 1.27e-180, "description": "Internal stop codon at 701099, 701189, 701639. Nonsense mutation around 701099, 701189, 701639.", "psc": { "uniref90_id": "UniRef90_C4XFY5", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "uniref50_id": "UniRef50_C4XFY5", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY5", "UniRef:UniRef50_C4XFY5", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY5" ], "product": "Energy-coupling factor transporter transmembrane protein EcfT" } }, "id": "IGIGPGHMMC_1251", "locus": "GCA000148625_01239" }, { "type": "cds", "sequence": "contig_1", "start": 701637, "stop": 701936, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCTCCTATTGGTCGTTATTATCCACATGAAACAATAATTCATAAAATTGATCCAAGGTTAAAATTGATAATCAATATTATTTATATTGTTTTAACTTTTATTGTTAATTACTTTGTCTCTTTAGCTGTTTTATTAGTACCTCTAATGATTGCATTTGTTATTGGTTCAAGAAAAATATATCCTTTATTTAGATTAATGATTATGCCTCTAATTATTGGTTTCTTTATTCTCTTTGTTAATATCTATACTATGAAGTTAAAACCTGACTCGGACTATACAAGAATAATACCCTGA", "aa": "MKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIP", "aa_hexdigest": "7a9ac08d0e5979782e99b999bd27eeb6", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11645.265499999985, "isoelectric_point": 9.9275671005249 }, "id": "IGIGPGHMMC_1252", "locus": "GCA000148625_01240" }, { "type": "cds", "sequence": "contig_1", "start": 701936, "stop": 702499, "strand": "-", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "nt": "TTGTTTGAATCTACTATTAAAGATGATATTGCTTTTGGACCAAGAAGCTTTGGTGTGCATAAAACTGAAGCTTATGAAAAAGCTAAAAAATATCTTGAGTTAGTAGGTTTGGATGAAAGTTACTTGAATAAATCACCTTTTGGTTTAAGTGGTGGTCAAAAAAGGCGTGTTGCTTTAGCTGGAATTTTAGCTATGGAACCTGACATTTTAATTGCTGATGAACCAACAGCCGGATTAGATCCAGTTGGAGTTAGAGAAGTTTTAGAAATTTTTAATAAACTTCATCAACAAGGTAAAACAGTTATTATTATTACACATGATTTGGATAATGTTTTAGAAGTAGCAAAAAGAGTATTGATTTTTAAAGATGGAAAAGTTGTTAAAGATGGCAATACTTATGAAGTATTAAGGAATACAAAATTCTTAAAAGAAAACAATATGGAACCTCCAAAATTATTAGAATTCGTTTCTAAATTAGAGGAAAGAGGCATTAGTGTGCCTAAAGTTACATCTATTGATGAACTAGCTGCATTCTTAAATAAAAGAATGAAAGGAGGTGCATAA", "aa": "MFESTIKDDIAFGPRSFGVHKTEAYEKAKKYLELVGLDESYLNKSPFGLSGGQKRRVALAGILAMEPDILIADEPTAGLDPVGVREVLEIFNKLHQQGKTVIIITHDLDNVLEVAKRVLIFKDGKVVKDGNTYEVLRNTKFLKENNMEPPKLLEFVSKLEERGISVPKVTSIDELAAFLNKRMKGGA", "aa_hexdigest": "5fb59806501a5f7e995bb330bac0b03a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003084074B", "query_cov": 0.983957219251337, "subject_cov": 0.8598130841121495, "identity": 0.614, "score": 239.0, "evalue": 2.23e-77, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI003084074B" }, "pscc": { "uniref50_id": "UniRef50_UPI003084074B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1253", "locus": "GCA000148625_01241" }, { "type": "cds", "sequence": "contig_1", "start": 702668, "stop": 702847, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAAATAAAAGTTAAAGATTTAGAACATATTTTTAGTCGTAGAACTCCTATGGAATTTAAAGCATTAAGTGGTATTAATGTAACAATCGATCAAGGCGAATATATAGGAATAATTGGACAAACTGGCTCAGGTAAAACTACATTCATAGAGCATTTAAATGCTCTTATTTTGCCATGA", "aa": "MQIKVKDLEHIFSRRTPMEFKALSGINVTIDQGEYIGIIGQTGSGKTTFIEHLNALILP", "aa_hexdigest": "b65577624dc4fe05820daa9de0b87817", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.576271186440678, "hmm_cov": 1.0, "evalue": 2.3131286456059e-08, "score": 30.756103515625, "start": 23, "stop": 56 } ], "seq_stats": { "molecular_weight": 6571.578499999998, "isoelectric_point": 6.70941104888916 }, "id": "IGIGPGHMMC_1254", "locus": "GCA000148625_01242" }, { "type": "cds", "sequence": "contig_1", "start": 702838, "stop": 703635, "strand": "-", "frame": 2, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_013354838.1", "SO:0001217", "UniParc:UPI0001E32F33", "UniRef:UniRef100_C4XFY7", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_C4XFY7" ], "nt": "ATGATTAAAGTACAGGACCTAGTTTTTTCTTATCCAGAATCAAAAACAAAAGCTCTCAATGGGCTTAATTTTACCATTCCAGATGGTAAATATGTTGCTATATTAGGTCACAATGGATCAGGTAAAAGTACATTCAGTAAATTGCTTGTTGCTTTGTATAAACCTTCTTCAGGTTCTATTGAAATAAATGGAACAGTTATTTCTAAAAAAACAATCAGAGAAATAAGAAAGAAAATTGGCATTATTTTCCAAAACCCTGATAACCAATTTGTTGGCGCTAGTGTTGAAGATGATATTGCCTTTGGTTTAGAAAATCAATGCATTCCACAAAAAGAAATGAAACCTATTATTGATGATTTAGCTGAAAAAGTAGGAATGAAGGACTATTTGACAAGAGAACCTCAATTCCTTTCAGGAGGTCAAAAGCAACGCGTTGCCATAGCTTCTGTTTTAGCTTTAAATCCTGAAATCATAATTTTTGATGAAGTAACTTCAATGCTTGATCCAAGAGGTAAAAGTCAAGTTTTAAAAATTATTAGAGAAATCCAAAAAACTCGTCAAAAAACTTTAATTTCAATTACTCATGATATGGATGAAGCTATTCAAGCTGATTATTGCTTAGTTTTTTCTAAAGGTCAAGTTGTTGCAAGTGGCTCACCTAAAGAAATTTTAAAAAATCATGAAATCATTGAATTAGCTAAAATTGATTCACCTTTTATTTATAAGCTCAGTGAAAAAATTGAAAATATTGAATCAACATATAATGAAGAGGAGTTATTAAATCAACTATGCAAATAA", "aa": "MIKVQDLVFSYPESKTKALNGLNFTIPDGKYVAILGHNGSGKSTFSKLLVALYKPSSGSIEINGTVISKKTIREIRKKIGIIFQNPDNQFVGASVEDDIAFGLENQCIPQKEMKPIIDDLAEKVGMKDYLTREPQFLSGGQKQRVAIASVLALNPEIIIFDEVTSMLDPRGKSQVLKIIREIQKTRQKTLISITHDMDEAIQADYCLVFSKGQVVASGSPKEILKNHEIIELAKIDSPFIYKLSEKIENIESTYNEEELLNQLCK", "aa_hexdigest": "7e7533a266060b3d782fa1567b3db590", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F33", "ncbi_nrp_id": "WP_013354838.1", "uniref100_id": "UniRef100_C4XFY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F33", "RefSeq:WP_013354838.1", "UniRef:UniRef100_C4XFY7" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY7", "uniref90_id": "UniRef90_C4XFY7" }, "psc": { "uniref90_id": "UniRef90_C4XFY7", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [], "id": "IGIGPGHMMC_1255", "locus": "GCA000148625_01243" }, { "type": "cds", "sequence": "contig_1", "start": 703984, "stop": 704148, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTCACGTGATGGAGACAATGCACCTATGGCTATTATTGAATTAGTTAAAGGCGACAAAGTTAAAACAAACAAAGCAGAAAAAACATCAAAAGACAAAGACACAAAAACTACAGCATCAAAAGCAACAAAAAGCAAAAAAGATGCTAAAGTAACTAAATAA", "aa": "MVSRDGDNAPMAIIELVKGDKVKTNKAEKTSKDKDTKTTASKATKSKKDAKVTK", "aa_hexdigest": "32932a419cbea1c214d45bd3906cd3f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5837.681300000006, "isoelectric_point": 9.874316215515137 }, "id": "IGIGPGHMMC_1256", "locus": "GCA000148625_01244" }, { "type": "cds", "sequence": "contig_1", "start": 704457, "stop": 705470, "strand": "-", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "RefSeq:WP_013354840.1", "SO:0001217", "UniParc:UPI0001E32F35", "UniRef:UniRef100_C4XFY9", "UniRef:UniRef50_D5E4Y4", "UniRef:UniRef90_C4XFY9" ], "nt": "ATGGAAAAAATGACAAAATTAGAGTACCAAAAGGTTACTAAATTAAATAATTCAACTTCAAATGTAACTGCATTTACTCTTCAACCACTTGAACGTGGTATGGGTCAAACATTAGGTGTTGCTCTTCGTCGTATTTTACTTTCAAACATTACTTCATTAGCATTATTTGCAATCAAAATTGAAGATGTTAATCATGAATTCCAAGTAGTTGATGGTTGCGTAGAAGACGTTGCCACAATTATTATGAATTTAAGAAAAGTAAAATTCCAATACAACCCAGAATTTGTTAAAGATGACGAAATTATTAAAGTAACATTAAAAGCTGATCAACCAGGAGAAGTTACTTCAAGATTATTAGAAGTAAACAACTCAAGTATTGAAATTTTAAACAAATCATTAGAAATTGCTCACTTATCAGCAAATGGTAAACACAAATTAAATATTGAATTATATTTAAGACCAGGTCGTGGCTTTATTTCTAATGAAGAAAATAAGAAATTACTTAATAACTCTTCAATTACAACTAGAATGGAATCAAAAATCAAAAACGGAATTTTTATTGCTACAGATAGTAACTTTAGTCCAATTGAAAAAGTTAACTACACAGTTGAAGAATTAAACTCATCATCAAACAAAATTGAAGAAAGATTAACCTTCTCATTCCAAACAGATGGAACAGTTGATCCTAAGAGTGCATTACAACAAGCTTGTGAAATCTTAGTTGGTCACTTCAAATTGATTGGTAATGTTGATGAAATGAAAGTAAATGTATTTGCAGAAGAAAGTGAACAAACTCAAGAAACAAACGACAATGATGTTGATATCAATTCATTAAATCTTTCGGTACGTTCATTGAATGCACTTAAAAGAATTGGTAAAACAAAGATTTCTCAAGTAGCTGAAATGTCACTTGAAGAATTAGAACAAGTTAAAAACCTTGGTCGTAAATCACTTGATGAAATTCAACAACGTTTAAAAGAATATGGATATGAACTAAGTAAAGGAGAAGAATAA", "aa": "MEKMTKLEYQKVTKLNNSTSNVTAFTLQPLERGMGQTLGVALRRILLSNITSLALFAIKIEDVNHEFQVVDGCVEDVATIIMNLRKVKFQYNPEFVKDDEIIKVTLKADQPGEVTSRLLEVNNSSIEILNKSLEIAHLSANGKHKLNIELYLRPGRGFISNEENKKLLNNSSITTRMESKIKNGIFIATDSNFSPIEKVNYTVEELNSSSNKIEERLTFSFQTDGTVDPKSALQQACEILVGHFKLIGNVDEMKVNVFAEESEQTQETNDNDVDINSLNLSVRSLNALKRIGKTKISQVAEMSLEELEQVKNLGRKSLDEIQQRLKEYGYELSKGEE", "aa_hexdigest": "e4a20ddfac56c0d6702415765ca2ce3c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F35", "ncbi_nrp_id": "WP_013354840.1", "uniref100_id": "UniRef100_C4XFY9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F35", "RefSeq:WP_013354840.1", "UniRef:UniRef100_C4XFY9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY9", "uniref90_id": "UniRef90_C4XFY9" }, "psc": { "uniref90_id": "UniRef90_C4XFY9", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_D5E4Y4", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4Y4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4Y4" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [], "id": "IGIGPGHMMC_1257", "locus": "GCA000148625_01245" }, { "type": "cds", "sequence": "contig_1", "start": 705762, "stop": 705875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACCAAAAAGAAAAATATAACAAATGGTGTAGCACACATTCACTCAACAAACCAAAATACTATTGTTACCTTTGCTGACGAAAAAGGAAACGTAATTGCATGA", "aa": "MARKTKKKNITNGVAHIHSTNQNTIVTFADEKGNVIA", "aa_hexdigest": "edc136720f859cda4a2c66c2182ccb8f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4051.5889000000006, "isoelectric_point": 10.171128654479979 }, "id": "IGIGPGHMMC_1258", "locus": "GCA000148625_01246" }, { "type": "cds", "sequence": "contig_1", "start": 705898, "stop": 706269, "strand": "-", "frame": 2, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02952", "RefSeq:WP_013354842.1", "SO:0001217", "UniParc:UPI0001A8C14C", "UniRef:UniRef100_C4XFZ1", "UniRef:UniRef50_P38017", "UniRef:UniRef90_A5IYW1" ], "nt": "ATGGCTAGAATTTTAAATATTGAAATTCCTAACAATAAACGTGTTGTTATCTCATTAACATACATTTATGGTATAGGAAGAACTAGAGCACAAGAAATTTGTGCTAAAGCAAAAATTGATGAAAATCTTAGAGTTAAAGATTTATCAGAAGAACAATTATCAGCAATTCGTGAAGTAGCTAAAGAATACACAACTGAAGGTGATCTTCGTCGTGAAACCAGTTTAAACATCAAACGTTTAATGGAAATCAAATGCTACCGTGGTATTCGTCACAGAAAAGGTTTACCTGTTAGAGGTCAATGTACACAAAAGAATGCTCGTACACGTAAAGGTCCTCGTAAAACAGTTGCAGGTAAAAAATCAACTAAATAA", "aa": "MARILNIEIPNNKRVVISLTYIYGIGRTRAQEICAKAKIDENLRVKDLSEEQLSAIREVAKEYTTEGDLRRETSLNIKRLMEIKCYRGIRHRKGLPVRGQCTQKNARTRKGPRKTVAGKKSTK", "aa_hexdigest": "b9ae51f6d5f1f476d803c12f5915d571", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C14C", "ncbi_nrp_id": "WP_013354842.1", "uniref100_id": "UniRef100_C4XFZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14C", "RefSeq:WP_013354842.1", "UniRef:UniRef100_C4XFZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ1", "uniref90_id": "UniRef90_A5IYW1" }, "psc": { "uniref90_id": "UniRef90_A5IYW1", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "IGIGPGHMMC_1259", "locus": "GCA000148625_01247" }, { "type": "cds", "sequence": "contig_1", "start": 706280, "stop": 706393, "strand": "-", "frame": 1, "gene": "rpmJ", "product": "50S ribosomal protein L36", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02919", "RefSeq:WP_013354843.1", "SO:0001217", "UniParc:UPI0001E32F37", "UniRef:UniRef100_A0AB32XCA5", "UniRef:UniRef50_P20278", "UniRef:UniRef90_A0A944MPI7" ], "nt": "ATGAAAGTTAGAGCTAGTGTAAAAAGAATGTGTAAAGATTGTCGTGTTATCAAACGTAAAGGAATTATTCGGGTAATTTGCTCACAACCAAAACACAAACAAAGACAAGGATAA", "aa": "MKVRASVKRMCKDCRVIKRKGIIRVICSQPKHKQRQG", "aa_hexdigest": "137a65f66db9c2840d9e402850c0e6b2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F37", "ncbi_nrp_id": "WP_013354843.1", "uniref100_id": "UniRef100_A0AB32XCA5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F37", "RefSeq:WP_013354843.1", "UniRef:UniRef100_A0AB32XCA5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCA5", "uniref90_id": "UniRef90_A0A944MPI7" }, "psc": { "uniref90_id": "UniRef90_A0A944MPI7", "gene": "rpmJ", "product": "50S ribosomal protein L36", "uniref50_id": "UniRef50_P20278", "kegg_orthology_id": "K02919", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20278", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20278" ], "product": "Large ribosomal subunit protein bL36" }, "genes": [ "rpmJ" ], "id": "IGIGPGHMMC_1260", "locus": "GCA000148625_01248" }, { "type": "cds", "sequence": "contig_1", "start": 706411, "stop": 706626, "strand": "-", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "GO:0003743", "GO:0006413", "RefSeq:WP_013354844.1", "SO:0001217", "UniParc:UPI0001A8C14D", "UniRef:UniRef100_C4XFZ2", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_C4XFZ2" ], "nt": "ATGGCAAAAGACGCAATCAAAATGAAAGCTGTTGTCAAAGAAGCTTATTCAACAGATGAATATGATGTTGAACTTGAAAACGGCATGCTCATTAAAGCTCACATTTCAGGAAAAATGCGTGTAAATCACATTCGTATTTTGCCTGGAGATACTGTCGATGTTGAAATTAGTCCTTACAATTTATCTTTAGGACGAATAACATATCGTCACAAATAG", "aa": "MAKDAIKMKAVVKEAYSTDEYDVELENGMLIKAHISGKMRVNHIRILPGDTVDVEISPYNLSLGRITYRHK", "aa_hexdigest": "af11d2dbf559feacad294a6fa64b008c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C14D", "ncbi_nrp_id": "WP_013354844.1", "uniref100_id": "UniRef100_C4XFZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14D", "RefSeq:WP_013354844.1", "UniRef:UniRef100_C4XFZ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ2", "uniref90_id": "UniRef90_C4XFZ2" }, "psc": { "uniref90_id": "UniRef90_C4XFZ2", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "IGIGPGHMMC_1261", "locus": "GCA000148625_01249" }, { "type": "cds", "sequence": "contig_1", "start": 706723, "stop": 707376, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_C4XFZ3" ], "nt": "ATGATAACTATTAAAAATGAACAAGAAATTATTAATATTACTCAAAGCTGCAAAATCCTGGCAGAAGTTAAACAAATAGTTTATGACTTTGTAAGACCAGGGGTTTCATTAAAAGAAGTGGATTCAGTCGCTTTTAATGAAATAGTAAAAAGAGGTGCACAACCAGCTTTTAAAGGCTTATATGGTTTTCCAGCAACCTGTTGCATCTCAATAAATGAAGAATTGATCCATGGAATTCCAAGTGATTACATTATTCAAGATGGCGACATAGTTAAAGTCGACATGGGATGTACTTACAAAGGAATGAACAGCGATAGTGCCTTCACTAAAGGTGTTGGTAATATCAATGAAAGTGATAAAAAAATAATTAGAGTGGCTCAAGAATCATTCTATGCAGGTTTTAATGCGATTAAACCTGGTGCAAGAGTGGGAGATGTTAGTCACGCTATTGGTCAAGTAATAAGACAAAATAATTTATATACACCAAGTCAATACTGTGGGCATGGAATTGGACGTGAAGTTCATGAAGACCCTTATGTATTTAATGATGGCCATGCTGGTAGTGGACCATTATTAAAAGATGGAATGGTAATTTGTATTGAGCCAATGATTTTACAACAAAATGCTAGAATCAAAGTTAAAAAAGATAAATGA", "aa": "MITIKNEQEIINITQSCKILAEVKQIVYDFVRPGVSLKEVDSVAFNEIVKRGAQPAFKGLYGFPATCCISINEELIHGIPSDYIIQDGDIVKVDMGCTYKGMNSDSAFTKGVGNINESDKKIIRVAQESFYAGFNAIKPGARVGDVSHAIGQVIRQNNLYTPSQYCGHGIGREVHEDPYVFNDGHAGSGPLLKDGMVICIEPMILQQNARIKVKKDK", "aa_hexdigest": "013c6f1a702865bb9e88b7aa9bb30814", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_C4XFZ3", "query_cov": 1.0, "subject_cov": 0.8645418326693227, "identity": 0.995, "score": 434.0, "evalue": 2.4e-153, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "IGIGPGHMMC_1262", "locus": "GCA000148625_01250" }, { "type": "cds", "sequence": "contig_1", "start": 707404, "stop": 708066, "strand": "-", "frame": 2, "gene": null, "product": "Adenylate kinase", "db_xrefs": [ "RefSeq:WP_013354846.1", "SO:0001217", "UniParc:UPI0001E32F39", "UniRef:UniRef100_C4XFZ4", "UniRef:UniRef50_F9UK13", "UniRef:UniRef90_C4XFZ4" ], "nt": "ATGATAAGTAAAATTAAACAACATATAGTTTTTATGGGGCCTCCTGGTGTAGGAAAAGGTACAGTGGCTGCTATAATAGCTGAAAAATATGGACTAGTACATGTTTCAACTGGTAATATCTTTCGTGAAGAAATAGCTTCACAAAGTGAACTTGGTTTACAAGTTAAAAAGATAGTTGAAGCAGGACAATATGTACCAGACGACATTACAAATACTATTGTTAAAAATAAAATTCAAGCTTTAATCAAAGCTAAAAAGATTATTATGCTTGATGGTTATCCACGTACTATTGACCAAGTTAACTTTTTAGATAGTATTTCAAACTTTAAATATGTAGCTCTTGAATTAAGTGCTCCTGAAGATGTTATTTTAAAAAGATTAAATGGACGTAGACAATGTCCTAAATGTAAAGCAAGTTTCCATATTGATTTTATGCCTTCAACTAAAGGCAAATATTGTGATAAATGTGGAGCTGAATTAATCTTAAGAAAAGACGACTCAATTGAAGCTATTAAAGTACGTCAAAAAGTTTATCATGATCAAACAGCACCATTGCTTGATTATTACAAAAAATATAATTTATTATTAACATTTGATGCTACAAGTGATCCAGAAACTATAGCAAAAAATATTGTTGAATCTTTAGCGAAATTAAAAAACTAA", "aa": "MISKIKQHIVFMGPPGVGKGTVAAIIAEKYGLVHVSTGNIFREEIASQSELGLQVKKIVEAGQYVPDDITNTIVKNKIQALIKAKKIIMLDGYPRTIDQVNFLDSISNFKYVALELSAPEDVILKRLNGRRQCPKCKASFHIDFMPSTKGKYCDKCGAELILRKDDSIEAIKVRQKVYHDQTAPLLDYYKKYNLLLTFDATSDPETIAKNIVESLAKLKN", "aa_hexdigest": "d16dd8f9994701b2e5cea4a7b2b1b031", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F39", "ncbi_nrp_id": "WP_013354846.1", "uniref100_id": "UniRef100_C4XFZ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F39", "RefSeq:WP_013354846.1", "UniRef:UniRef100_C4XFZ4" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ4", "uniref90_id": "UniRef90_C4XFZ4" }, "psc": { "uniref90_id": "UniRef90_C4XFZ4", "product": "Adenylate kinase", "uniref50_id": "UniRef50_F9UK13" }, "pscc": { "uniref50_id": "UniRef50_F9UK13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK13" ], "product": "Adenylate kinase" }, "genes": [], "id": "IGIGPGHMMC_1263", "locus": "GCA000148625_01251" }, { "type": "cds", "sequence": "contig_1", "start": 708063, "stop": 708287, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTTTAGTATCAGTTGCTCTTGAAACTATTCAACAATTCATGGCACGCCGTAAAAGTCAACATCTTGCAAAACAAAAACGTCTTTCAAAAGAAGCTGCAGATGTGCTTAACTTCCAAAACAAATATCAAGAAAATGATCCTTTTGCAACAAATGATCAAAAATCTGAAGACGTTTCAAAAGAATATGACAAATACAAAAATGGAGATGGCTTATTATGA", "aa": "MMILVSVALETIQQFMARRKSQHLAKQKRLSKEAADVLNFQNKYQENDPFATNDQKSEDVSKEYDKYKNGDGLL", "aa_hexdigest": "1c758825e9f37433c9856efbff4ba77e", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8595.625100000001, "isoelectric_point": 7.965729713439941 }, "id": "IGIGPGHMMC_1264", "locus": "GCA000148625_01252" }, { "type": "cds", "sequence": "contig_1", "start": 708327, "stop": 708581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTCACTCAACCACTAGGCTTGTCATTATTGATTCTAATTACATTCTTCTTTANTTACATAATTGGATTGCAACAATCAAGAATTGACAAGATTTCAGAAGACTTTGCAAAGAACTCAACATTTATTCCTGGTATTAGACCTGGAGAAGAAACTGAAGATTATTTAATAGGTGTTGTTTTAAGACTTTGCACCTTCAGTGGTTTCTATTTAGTATTCTTAGCTTCATTCCAATACATAATGATTTTATGA", "aa": "MQFTQPLGLSLLILITFFFXYIIGLQQSRIDKISEDFAKNSTFIPGIRPGEETEDYLIGVVLRLCTFSGFYLVFLASFQYIMIL", "aa_hexdigest": "7ba35aedab0f8659b06ff07e63daf054", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": null, "isoelectric_point": 4.715101432800293 }, "id": "IGIGPGHMMC_1265", "locus": "GCA000148625_01253" }, { "type": "gap", "sequence": "contig_1", "start": 708523, "stop": 708523, "strand": ".", "length": 1, "id": "IGIGPGHMMC_1714" }, { "type": "cds", "sequence": "contig_1", "start": 708594, "stop": 709454, "strand": "-", "frame": 3, "gene": "secY", "product": "Preprotein translocase subunit SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "nt": "TTGCTTTATACATTATTGTTGTTATCAATCTATATTTTAATGACAACAATCAAAGCTCCTTTTGTTAAATTATCTGATGTAAATATTAATGATGACCCATTCTTAAATACATTAAACCTTGTTGGCGGTGGAGGTCTTAAAAACTTCTCACTTGCAGCACTTGGAATTAGTCCATTCATTAATGCCAGCTTGATAATGTCTCTTTTACAAACCAAACTTTTCCCTCCAATTCAAAAACTTAGTCAAAGTGGACCACAAGGTCGGAGAAAATTAAATATCATTACAAGAATTTTGACTTTGTTTATTGCATTCCCTCAAGCTATCTTGCTTACTAAATCACTTAGTTTAGGTGACAATCCATTTATTGGATTTACTCCAATTCAAAAACTCCCTCGAGAAGTTACAATCTACTTAATATTGCCTTTAATCTTAATTGGTGGATCATTGTTCTCATTATTCATTGCTGAACAAATTACAGATAAAGGTTTAGGAAATGGTACAAGCTTAATTATTTTTGTTGGTATTGCATTCCAATTACCAAGTCAATTTAAAGGAGCATTTAGCTTATTTGTTGGTAGTGAAAATACAGCAGCAATTTTTGTTGGAGCATTAAAATTCTTAACTTACTTATTTGTTTACTTTGCATTGTTATTTATTATTGCTTTAGTTTACAATGCTGAAAGACATGTACCTATTCAACAAATTGGTGCTGGACGTAGTAGAAATATTAATGAAATGGGTAAATTGCCAATTAAATTAAATCCCGGTGGAATTATGCCAATAATCTTCGCCTCAATGCTAGTCAGCTTCCCAATTATGATATCTAGAATACTTCCAAAAGGTAATTCTGGCAAATTATGA", "aa": "MLYTLLLLSIYILMTTIKAPFVKLSDVNINDDPFLNTLNLVGGGGLKNFSLAALGISPFINASLIMSLLQTKLFPPIQKLSQSGPQGRRKLNIITRILTLFIAFPQAILLTKSLSLGDNPFIGFTPIQKLPREVTIYLILPLILIGGSLFSLFIAEQITDKGLGNGTSLIIFVGIAFQLPSQFKGAFSLFVGSENTAAIFVGALKFLTYLFVYFALLFIIALVYNAERHVPIQQIGAGRSRNINEMGKLPIKLNPGGIMPIIFASMLVSFPIMISRILPKGNSGKL", "aa_hexdigest": "99a06d812274812e5503f81b606c180e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CD17B9", "query_cov": 0.9090909090909091, "subject_cov": 0.8843537414965986, "identity": 0.604, "score": 291.0, "evalue": 2.09e-95, "valid": false, "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A3B0PVK7" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PVK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "product": "Preprotein translocase subunit SecY" }, "genes": [ "secY" ], "id": "IGIGPGHMMC_1266", "locus": "GCA000148625_01254" }, { "type": "rRNA", "sequence": "contig_1", "start": 709873, "stop": 712755, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9856410256410256, "score": 2772.1, "evalue": 6.1e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TTTTAAATAAGGAAGAGTTTGTGGTGGATGCCTTGGGTCTGAAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGTGGAGCCGGAAATATGCTATGAAACGGGGATTTCCGAATGGGGAAACCTAACTAGGGTAATGCCTAGTTGCCTTGTTCTGAATTCATAGGAACTTGAGTGAGACACCTTGTGAACTGAAACATCTTAGTAGCAAGAGGAAGAGAAAATAAACAATGATTTCTTTAGTAGCGGCGAGCGAACGAGAAAGAGCCCAAACCCAGCAATGGGGGTTGTAGGACGATCTACATGAAGTTACAAAACTTAATGATAGCAGAAGCTTTTGGGAAGAAGCGGCATAGAGGGTGATACCCCCGTATGTGAAATTGTTAAGTCTTCTGATCGTATCCTGAGTAGGGCGGGGCACGTGGAACCCTGTCTGAATCTGCCGGGACCACCCGGTAAGGCTAAATACTAATCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGAGAGGGGAGTGAAATAGATTCTGAAACCACTTACTTACAATTAGTCAGAGCCCGTTTATGGGTGATGGCGTACATCTTGCAGTATGGACCGGCGAGTTATGACAGCATGCGAGGTTAAGCAGAAAAAAGCGGAGCCGTAGAGAAATCGAGTCTTAATAGGGCGTCTTAGTATGTTGTCATATACCCGAAACCATGTGATCTATTCATGAGCAGGCTGAAACTTGGCTAACCCCAAGCGGAGGGCCGAACCGTAGTACGCTGAAAAGTGCCCGGATGACTTGTGAATAGTGGAGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCCTCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAGTAGTGGTGGTAGAGCACTGAATATGGAATGGCCTCGCCTAGGGGTACTGACTATAATCAAACTCCGAATACCGCTATGTATTGCTATGCAGTCGGAACCGGGGTGCTAACGTCCCGGCTCGCGAGGGCAACAACCCAGATCGCCGGCTAAGGTCCCAAAATCGTGTTAAGTGAGAAAGGTTGTGAGGTTTCATAAACAACTAGGAAGTTGGCTCAGAAGCAGCCACCTTTTAAAGAGTGCGTAATAGCTCACTAGTCAAGAGACCTTGCGCCAACAATTTAACGGGACTAAAACACGATACCGAAGCCGCGGGTAATTATTTTACGTTAGAGGAGCGTTCCTAGGGCAATGAAGTCAGACCGTGAGGACTGGTGGAGCGCTAGGAAGTGAGAATGCCGGTATGAGTAACGATTCGTAGTGAGAATCTACGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGACCTAAGACGAGGCTGAAAAGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTATAATAGTGATGGAGTGACGGAGGAGGATAATCTTACCACTTACTGGATTGTGGGGTAAGCGTTCACTGGTGACTGTAGGCAAATCCGCAGTCTATAACCGGGAGGCGTGATGCATAGGGTCAAACCGAATTAGATGATTCCACACTTCCAAGAAAAGCTTCTAAACTTAATATTATGGAAACCTGTACCGAGAACGGACACACGTCCCCGAGATGAGTATTCTAAGGCGAGCGAGAAAACTAGTGTTAAGGAACTCTGCAAAATGACCCCGTAAGTTCGCAAGAAGGGGCGCCTAAGGTAACACTTAGGCCACAGTAAATTATGAGGGGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAACCGCAAGGTGATGTATAGGGGGTGAAGCCTGCCCAGTGCCCGAAGGTTAAGTGGATGCGTTAGCATTTGCGAAGCGTTGAAATGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGCGCAATGATCTCTCAACTGTCTCAACACTAGACTCGGTGAAATTATGGTCCCAGTGAAAACGCTGGGTACCCGCATCAAGACGAAAAGACCCCATGGAGCTTTACTACAGTTTCGTATTGGAACTTGGTCTAACATGTGTAGGATAGGTGGGAGACTTTGAAGCGAGGACGCTAGTTCTCGTGGAGTCATCCTTGAAATACCACCCTTGGTATATTGAGTTTCTAACATGCCATCATCATCAGGTGGGTGGACAGTGCGTGACGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGAGTAACGGAGGCGTTCAAAGGTACACTCAGTACGGTCAGAAACCGTATGTAGAGCGCAAAGGTAGAAGTGTGCTTGACTGTGAGACTTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGTTGACCAATACTAATAAATCGATCGGTTTAAAAGT", "id": "IGIGPGHMMC_42", "locus": "GCA000148625_01255" }, { "type": "rRNA", "sequence": "contig_1", "start": 713069, "stop": 714581, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9869536855838226, "score": 1508.8, "evalue": 4.4e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGGCGTTAAATTTTGGGGCTCAACCCCAAAACGCGTTGGATACTGGCAGGCTAGAGTTGTGTAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAAGATGGCGAAGGCAGCTAACTGGACATATACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATCATTAGCTGATGGGTAACTCATCGGCGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTACGTTCGCAAGAATAAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATGGAGACATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCTGCAACGAGCGCAACCCTTATCCTTAGTTACTACCATTCAGTTGAGGACTCTAAGGAGACTGCCCGAGTAATCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCCGGTACAAAGAGAAGCGAAGTGGTGACATGGAGCAAACCTCAAAAAACCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTATAAACAAACTGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "IGIGPGHMMC_38", "locus": "GCA000148625_01256" }, { "type": "cds", "sequence": "contig_1", "start": 714832, "stop": 715965, "strand": "-", "frame": 2, "gene": null, "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "RefSeq:WP_013354848.1", "SO:0001217", "UniParc:UPI0001E32F3B", "UniRef:UniRef100_A0AB32XCZ2", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_C4XDW7" ], "nt": "ATGTACAATAAGATTTTAATTAGATATGGTGAATTGACTCTAAAAGGCAAAAATAGAGTTAATTTTATTGATACTTTAGCTTCAAATATTCGTCATAAAACTAAAGATAAATTAGAGGTTGAATTTGACCGTATGTTTTTGCCTTATAGTCAAGAAAATTTAGATGCATTGCAATACATTTTTGGTATTACTTCTTACTCACCTGTTATTATTTGTGCTAATGATATTGAAGAAATTAAAAAGCAAGCTTTAAATTTAGCAAAAAATAAAAATGGAACTTTTAAAGTTGCTGCTCGTAGAAACAATAAAAGTTTTCAATACCAATCAAGTCAAATTAACAATATAGTTGGCGGTTTTTTATTAGAAAACAACCAAAATTTAAAAGTTGATGTTAATAATCCAGACTTTATTGTTTATATTGAAGTTAGAGGCAAATTTACTTATGTTTTTAGTGATTTTGTCCAAGCTTTAGGCGGCCTCCCCGTTGGTGTTAGTGGGCAAGTATTGCACTTAATGAGTGGTGGAATTGATAGTCCTGTTGCTGCTTTTCAACTTATGAAAAGAGGTCTTAAAGTTAATTTCTTATCTTTTATTACACCTCCTCAAACTGATCAAGCAACAGTAAATAAAATGAAAGAATTAATCAAAATATTAACTAAATATCAAAACACAAGCACTTTTTATTTAGCTAACTATTCTACTTTAATGAACTACATAGCTTTAGTTTCAAATCAAGCTTATAAAATTACTCTTATGCGTCGGAGCTTTTATCGTATTGCTGCTAAATTAGCTCAAAGTAAAAAAAATCTAGCCATTTCTAATGGCGAAAACTTAGGTCAAGTAGCTTCACAAACTTTAGAATCACTTTATACAATTAGTCATGATATTAACATGCCAATTTTGCGGCCACTATTAACTAATGACAAAATTGAAACAATCAATTTAGCTAAAAAAATTCAAACCTATGATCTTTCAATTACTAAATGTAATGAAACCTGTGAGCTTTTTGCTCCTAAAGAACCAGTAACTAAACCTAATGTTCAAGAAGCTCTTAAATTAGAAGAAGAATTAAACATGATTAATGACTTAGAAAAAGAATGCTTAGAAAAAAATATTGAAATAATCAAATTCTAA", "aa": "MYNKILIRYGELTLKGKNRVNFIDTLASNIRHKTKDKLEVEFDRMFLPYSQENLDALQYIFGITSYSPVIICANDIEEIKKQALNLAKNKNGTFKVAARRNNKSFQYQSSQINNIVGGFLLENNQNLKVDVNNPDFIVYIEVRGKFTYVFSDFVQALGGLPVGVSGQVLHLMSGGIDSPVAAFQLMKRGLKVNFLSFITPPQTDQATVNKMKELIKILTKYQNTSTFYLANYSTLMNYIALVSNQAYKITLMRRSFYRIAAKLAQSKKNLAISNGENLGQVASQTLESLYTISHDINMPILRPLLTNDKIETINLAKKIQTYDLSITKCNETCELFAPKEPVTKPNVQEALKLEEELNMINDLEKECLEKNIEIIKF", "aa_hexdigest": "eb42021110b1507bb89351709575da15", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32F3B", "ncbi_nrp_id": "WP_013354848.1", "uniref100_id": "UniRef100_A0AB32XCZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F3B", "RefSeq:WP_013354848.1", "UniRef:UniRef100_A0AB32XCZ2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCZ2", "uniref90_id": "UniRef90_C4XDW7" }, "psc": { "uniref90_id": "UniRef90_C4XDW7", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2" }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [], "id": "IGIGPGHMMC_1267", "locus": "GCA000148625_01257" }, { "type": "cds", "sequence": "contig_1", "start": 715955, "stop": 716149, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGTTTAAGGCTTATTTATATAAGAAAATGACAGAAGAACTCATGTTAAAAAGTATGCATTATCGATTTAATAATCCAGACTTTAATGAACTCTTAATTTCAGTCAATAAACACATGCGAGCTAAGAAATTTGATGAAGCTTTCTCACTACTAGCAACCTGCATGAAAAAAGGAAAAAAATATGTACAATAA", "aa": "MTFKAYLYKKMTEELMLKSMHYRFNNPDFNELLISVNKHMRAKKFDEAFSLLATCMKKGKKYVQ", "aa_hexdigest": "b2cd6594d2a6f31b592b1be8f56334df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7731.1562, "isoelectric_point": 9.718689537048338 }, "id": "IGIGPGHMMC_1268", "locus": "GCA000148625_01258" }, { "type": "cds", "sequence": "contig_1", "start": 716333, "stop": 716737, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAAGAATCAAAAAAATCTATGCTGATTATTATACTTTAAACAAGAAAACTGCTCTTAATGCTGAGTTTCAAGATTTCATAAATAATGAATTAGAAAACATAGGCAAAATGATTAATGAGCTTGATCAAAAATTAAGCTATATTTCAATTGATTTAGATGATTTTGACAGCAATTTTGTAACCAAAATTTCACAAGAATTGTTACAACTTAGAAACCAATATGATAGCATTAATAAAGCTAATAAAGCTAATGGCAATGTTAGTTTAATGGAGATTCAAACACTACTTCATGGCATAATGGAATTAATTAAAAAATGCAATGATAAAATTGAGATTTTTAACCTCGATACTTATCAAAAAAATTACAATGCTTACTACTTAAAAATTTTGGAAACTTGA", "aa": "MQERIKKIYADYYTLNKKTALNAEFQDFINNELENIGKMINELDQKLSYISIDLDDFDSNFVTKISQELLQLRNQYDSINKANKANGNVSLMEIQTLLHGIMELIKKCNDKIEIFNLDTYQKNYNAYYLKILET", "aa_hexdigest": "487a91b0397ca8ef52f8c83f23043400", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15819.83819999999, "isoelectric_point": 5.021918296813964 }, "id": "IGIGPGHMMC_1269", "locus": "GCA000148625_01259" }, { "type": "cds", "sequence": "contig_1", "start": 717131, "stop": 717556, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAATTAAAATTATTGTACCAATAGTTATTTTGGCCTGTTTAGGTCTTTTGGGCATTTTTATTTTCCTTTATATTAGAAAAACTAATCTTAATAAATACATTAAATTTTTAGATGATTCAACTAAAAATATTAAAAATGATCTAACAGGACGTAACGCAACAATTCAACGTTTTATTACTTTATCAAATACTCAAGAAACATATAAAGCTGCTTTAAATCAATTAAAAAGTTTAGACAACAAATTAAAAAAAATAATTGTTGATTTAAATGATAAATATAATGCATTAAGAAAAGCGGCTAAAGCTTATAAACTTAAAGAAGCTAAAAAACTTTACTTAGAAATTTTGCCTAAATATGAAGAATGCATTTCAATGCATAAACAATTTGATGAAAAAACTAAGAACTTAAATAAACATTGA", "aa": "MQVIKIIVPIVILACLGLLGIFIFLYIRKTNLNKYIKFLDDSTKNIKNDLTGRNATIQRFITLSNTQETYKAALNQLKSLDNKLKKIIVDLNDKYNALRKAAKAYKLKEAKKLYLEILPKYEECISMHKQFDEKTKNLNKH", "aa_hexdigest": "7884f6c7642278e0466e9921c9a21e0a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16434.35519999999, "isoelectric_point": 9.778967475891111 }, "id": "IGIGPGHMMC_1270", "locus": "GCA000148625_01260" }, { "type": "cds", "sequence": "contig_1", "start": 717660, "stop": 717860, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013354850.1", "SO:0001217", "UniParc:UPI0001E32F3D", "UniRef:UniRef100_A0AB32XC94", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_C4XDW9" ], "nt": "GTGGCTATAGTTCCAAAACGTAAGACATCTAAACAACGTAAACACAAAAGACGTACCCATGATGCTTTACCTGTTCAAAATCTTGTTGCATGCAAAAACTGTTCTCAATTAATTCAACAACACCGTGTATGTGAATCTTGTGGATTTTATAAAGGTAAAAAAGTTGAAGGTTACAAAAGTTTAGATATGCGTAATGCTTAA", "aa": "MAIVPKRKTSKQRKHKRRTHDALPVQNLVACKNCSQLIQQHRVCESCGFYKGKKVEGYKSLDMRNA", "aa_hexdigest": "ed8e2872a40e75b33585b60cb4069913", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F3D", "ncbi_nrp_id": "WP_013354850.1", "uniref100_id": "UniRef100_A0AB32XC94", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F3D", "RefSeq:WP_013354850.1", "UniRef:UniRef100_A0AB32XC94" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC94", "uniref90_id": "UniRef90_C4XDW9" }, "psc": { "uniref90_id": "UniRef90_C4XDW9", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "IGIGPGHMMC_1271", "locus": "GCA000148625_01261" }, { "type": "cds", "sequence": "contig_1", "start": 718023, "stop": 718769, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATTAATGAACAACTAAAGAGTGCAAATTATCAAATTTATCAAAATGAAATGAAGCAAATTTTAGATGATTTTTATAGCAAAAATGGAGATTATAAAAATCAAAAAATTCAATTTACATTACCTATTTTTGCTGAAAAGAGTTCATTTATTGACAGTTTATATAAAAGAATAGCCTTCTTGTACAACAACCTAGACAATCGTTTAAATGTTAATTTTGAATATGCTTCAAAAGAAGCAAATAATTACTTTTCTAAATATCAAAACTTTAGTGCAGTTGATAATTCATTTAGTAACTATTTAATTAATTTAATAACAGCAAATAACCAATTCTTTTTAAATTTATTCTTATATTCAAAAGTCAATTTAGAATCAATTAACTATGATAAAAATATCAAAAAAATTATTTCAACAATAGAAGAAACAATTAATAAAGTTGATTACTTTAAAAATTATGAGTTGCAAAAAATTAAAGCAAACAGTTCACTTTTAGATCAAATTTTGCAAAAAAATAATTTGAAATTGAAAGAATTCAAAGCTAGATTTAAAGAGAAAATTCAAGCTTTAAATTTTGATCAACAATTCAATATTTTAAAAAGTATTGACTCATTTTTAGGAATCAGAGTAACTGAAGATAATTTCCTTTTTACTAACGAATACCAAAAATCAATAGTTCAATACTTCTACACAAAACCAATAAATCTAGATGGTTTTACTTATTTTCAAGATATTACTTTTAACTAA", "aa": "MDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN", "aa_hexdigest": "b6e3b16ac68c30c558a6730a4e6cb1bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29635.134500000044, "isoelectric_point": 8.384322929382325 }, "id": "IGIGPGHMMC_1272", "locus": "GCA000148625_01262" }, { "type": "cds", "sequence": "contig_1", "start": 718974, "stop": 720047, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0", "UniRef:UniRef90_C4XDX0" ], "nt": "TTGAAGGATAATAGTCAAAAAATTTTTAATAGTGATGATGTTAAAGAAAAAGTTGATCAAGCTCTTCAAAGTAGTACTAAAGTTAATGAAGATGGAGCATTAAGCGGTATTAAACAAATTTATTCTGATGAAAATAATAATATAAATTCTAAAGAATTTTTTGAAGCTTTAAATAGTGCAACTAAAATTGAATTTGAATTAAAAGACGATGTTTATTATTCAGATTATAAAGGCAAAAAAACAAATCATAAATTACAAGCCCAAGACTATTTAACTAGCTTTAATTTGAAACAAAATCGTGAACAATTTTCAGCTCTTTTAGACAACTATTCTTTGAAAAATTTAGGCCAAAATAGCATCCAAAATAACAAGTTAATTTTTGAATTAAACGATGCTCAAAAAAGCAAAAATAATAACTTTATTAAACTATTTACAACTCAAGAAATTACTAATAATTTGATCTTCAATCCAATATCAACAGACTACTATAAAAATAATAAAAATAGTTATGGCAAAAATTACCAAAACATCTGTTTTATAGGTTATTATGTTTTAAATAAGAACACTGTTGATGAGCAAATTTTTATTAAAAATAATAACTCTGCTTTAGATAGTTTTAACCAAAATGAAAAGCAAAATATTCTTAATAAAGTGACTATAAAATATAATCCAATTCAAAGCGATGAGCCTACCTTTAGATTACAAAGTTATAATGCATTTAAACAAAATCTTTTAAGTGAAGCTTCTTATGATATTTTTAACCAATTGCAAAAAGATGAAATAGATAATTTCTCTAATCTTTTTGGTGTTACTTATACATTATTTAAACCAAATAATAGTAATGTTATAAGCTATTTTTACAATGTTAATCCATATGCTAAAAATAACAAAGATTTCAAATTTAATGAAGCTTTTAGCTTACTATTTTATAATCAGAAATTAAAAGAATTAAAAGATCAAAATTTAGTAAATAAAAAATATTATGAACCACATCAAATTGTGTTCAGAAATATTTTAAATAACTGTATTAATCAATATTCATTTATTAAAAATTTGAATCATAATACTTACTGA", "aa": "MKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQSDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLKELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTY", "aa_hexdigest": "07c35a68c526c3f757b458f0ae797c56", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 718671, "stop": 720347, "observations": { "mutation": [ 718976, 720152 ], "stop": [ 718976, 720152 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX0", "query_cov": 1.0, "subject_cov": 0.45535714285714285, "identity": 0.992, "score": 681.0, "evalue": 1.92e-240, "gene_start": 1, "gene_end": 357, "reference_start": 111, "reference_end": 467, "reference_sequence": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNPKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIRWKSLGKSKFDHLNKFFYKTTTRFLEFYLAKSITVTLKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQNDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLNELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTYWNSFASQSLNYDSSNNSGILDNQNVNFLDDINRVYDYHFDYKTNKFDNKIIDFNGSDGLIKHSINSKNILDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN" }, "paralog": false, "identity": 0.989247311827957, "score": 1108.0, "evalue": 0.0, "description": "Internal stop codon at 718976, 720152. Nonsense mutation around 718976, 720152.", "psc": { "uniref90_id": "UniRef90_C4XDX0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX0", "UniRef:UniRef50_C4XDX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0" ], "product": "Lipoprotein" } }, "id": "IGIGPGHMMC_1273", "locus": "GCA000148625_01263" }, { "type": "cds", "sequence": "contig_1", "start": 720150, "stop": 720377, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAAATTAAAAAATATGGCAGCTTATTTTTAATTAGTGCAACACTTACTTCTAGTGTTGCTACTCTTTCATGCAACAATATTAGACAAGTTAATCATAAAGACAGTTATATTAAAAAATATAATGAACCAAACAAAATTAAAGACAATTTTATTATTAACAAAGTTAAAAGAATTGATAATGATTTTGAGAGTTTATTAACTGCACCTTTAATTAGATGA", "aa": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNHKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIR", "aa_hexdigest": "d5b00b52498b71d06f9bc84be999a281", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8701.086899999995, "isoelectric_point": 10.00628299713135 }, "id": "IGIGPGHMMC_1274", "locus": "GCA000148625_01264" }, { "type": "cds", "sequence": "contig_1", "start": 720386, "stop": 720595, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTAGCTTCATTAGCTTTCTTTAATATTTATAAAATTGATCAAAGCTTAAATATCGGAAACGTTTTTAAAGATATAGTTGACAATCTAAAAAATGTTGAATATACAATTTTTGTTTCAATATTAGCAAGTTTTTATGTAATATTAATAAAATTCTTAGGTATAAATTTCTTTATTGCGAGTGTACCTAAATTAGAAAAGTAA", "aa": "MLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILIKFLGINFFIASVPKLEK", "aa_hexdigest": "9dd3a70238378852cfe76fec9918fd75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7849.268199999995, "isoelectric_point": 8.006925010681151 }, "id": "IGIGPGHMMC_1275", "locus": "GCA000148625_01265" }, { "type": "cds", "sequence": "contig_1", "start": 720674, "stop": 721534, "strand": "-", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1", "UniRef:UniRef90_C4XDX1" ], "nt": "ATGGCATTTAATCATGAGCACCACCATTCTCACGAAAAAGCAGGCCAAAGTCATATTGAATTTGAAAAAAGTCTTTTCACTTTAACTCACCATAAACACTTCCAAGTAAATATCAGCAATAACAAAAGTGAATTTAAACAATACTTAATTCGTTTCTTTAGCAAAAAGATCAACATTGCTTTAGTTTCAATTATTGGAATCTTCTTACTGTTTTTGACTTTAAGTGCTATTTTTAGCCCTTATTCGCCAAAAACTAGTGTCATCGATTCACAACTTGCTTATAATTTGCCTAACTACTTAAATGCAAGTATTACTAAAAAGTTTGCACAAAGTGACCCCTTATATCAATTTATTTTGCAAACTCAAAAAGATCATCCTAATTTAAAAATAGTCATAAATGAAACACCTGATTTTGACTTAGTTAATTTAACTTACAATCCATATCAATTGCTTTATGCCTTAAAAGGCACTAACTATATCTTTTTATATGGTACCAATTCTCAAGGTATAGACCGCTTTAGTTTCTTTATTCATTCATTTGGCATAAGTATTTTAGTAACATTTGTGGCTGCTTTAATCCAATTATTTTTAGGGACCTTTTTAGGTTCAATAGTGGCTTATTATTCAAATAAAAGCAGTGCTAAATTTAGTTACTATTTAATTAGTACTATTAATATTGTGCCTTTCCTTATTGTAGTTTTCTTGATTTTTAAAATAAGTTCATATAACCATCTTAATGCTATATTAATTTTAGGTTTTATTGGTTCCCTTAGCTTCTTTTATTCAAGTTATTCAATAGGGCTTGAAATCAAAAACAGTGAATTTATTTTAGCTTACAAAGCTAATGGCTTGAGTAATTGA", "aa": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSN", "aa_hexdigest": "b20b7d7adee4e54de4dedb54bcbec789", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 720386, "stop": 721534, "observations": { "mutation": [ 720637, 720676 ], "stop": [ 720637, 720676 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX1", "query_cov": 1.0, "subject_cov": 0.7486910994764397, "identity": 1.0, "score": 540.0, "evalue": 5.68e-192, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSNWRIIYKIIFIHCLWRNIALVSDSLSLNMLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILVKFLGINFFIASVPKLEK" }, "paralog": false, "identity": 0.9921465968586387, "score": 740.0, "evalue": 2.2e-273, "description": "Internal stop codon at 720637, 720676. Nonsense mutation around 720637, 720676.", "psc": { "uniref90_id": "UniRef90_C4XDX1", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX1", "UniRef:UniRef50_C4XDX1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "IGIGPGHMMC_1276", "locus": "GCA000148625_01266" }, { "type": "cds", "sequence": "contig_1", "start": 721524, "stop": 722156, "strand": "-", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2", "UniRef:UniRef90_C4XDX2" ], "nt": "ATGACTGATTTAGTCCTCTCATTTATTGTCTTTATTTTAGCTGCTATTCCTACTTTTATCTTTGCACCAATAATGCTAATTATTTCAGAAGTCAATGACTTACCAGTTAATTTTATAGAGCCATCAGCTTTTGGTTTTGGTTATACATTGCTTTCTTTAATACTTCCTATTTCACTTTTATCTTTAGGAATCATTGCATTCTTTGCAACCATTACCAAAAATAATCTTGTTCAAATTCTAAAAAAAGATTATGTAATCACTTTAAAAGCAAATGGCTTAAGTACTTTACAAATATTTAATAAAGCTGTTATTAAAAATTTATTTATTTCATTAGTTAACCAAATAGTGCCAATTTTAGTTTTAACTATAAGTTTTAGCTTAATTATCGAACGTATTTTTCAAATACCAGGTCAAAGTGTTATTTTAATTAGCATGTTTGATCAAAAAGAAATTAATGTTATTATGGCTTTAGTATTTTTTAAAGCTTTGCTTTTATTCTTATTAGCTTTTATTTGTGAATTAACTTATGATTTATTGCAAGTTGAAAATGGTTATAATTTTAATTTCAATTTTAACTTCAAAGAAAATAGAGAAAAAGCTAGATTCAGAAAGGAGGCGCATAATGGCATTTAA", "aa": "MTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKARFRKEAHNGI", "aa_hexdigest": "f638ac15bf054f792df274ad072714b3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 721524, "stop": 722456, "observations": { "mutation": [ 722180 ], "stop": [ 722180 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX2", "query_cov": 1.0, "subject_cov": 0.6306306306306306, "identity": 0.99, "score": 350.0, "evalue": 3.49e-119, "gene_start": 1, "gene_end": 210, "reference_start": 124, "reference_end": 333, "reference_sequence": "MKRFFYVERWIFMNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGIWLGYKNNKMTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKDRFRKEAHNGI" }, "paralog": false, "identity": 0.9806451612903225, "score": 566.0, "evalue": 6.09e-207, "description": "Internal stop codon at 722180. Nonsense mutation around 722180.", "psc": { "uniref90_id": "UniRef90_C4XDX2", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX2", "UniRef:UniRef50_C4XDX2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "IGIGPGHMMC_1277", "locus": "GCA000148625_01267" }, { "type": "cds", "sequence": "contig_1", "start": 722178, "stop": 722489, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTAGCAAATACTTTTTCAAAAAAATTCTACTTACATTCTTAGGTATTTTTATAACAGTTACCCTTGGCTATTTTGTTATAGCTTTATTTATTAATAACTCACAAATAAACAGAGAACCAATTATCAAAAATTATTTCACTTTCTTAGGTTCTATTTTTAGTGGTTTTGGTAAACCATTTTCAACTAATAATAGTGGTTTCAATTCATCGTTAGAATTATTCTTCCATTATTATAAATTTAGTTTACTTTTTGTAAGCATTTCTTTTGTATTTAGCTTTTTTATAGGTGTTTTTGTCGGAATATGA", "aa": "MNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGI", "aa_hexdigest": "4782be8f2d41c46e4e81b958565d33d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11920.856699999988, "isoelectric_point": 9.756339073181149 }, "id": "IGIGPGHMMC_1278", "locus": "GCA000148625_01268" }, { "type": "cds", "sequence": "contig_1", "start": 722543, "stop": 722812, "strand": "-", "frame": 1, "gene": null, "product": "Phosphocarrier protein HPr", "db_xrefs": [ "RefSeq:WP_013354854.1", "SO:0001217", "UniParc:UPI0001A8C336", "UniRef:UniRef100_C4XDX3", "UniRef:UniRef50_A0A1H9FWZ8", "UniRef:UniRef90_A0A3P8MEF8" ], "nt": "ATGAAAGAATTTACAGCTTCAATTATAGATCCAATTGGTCTTCACGCAAGACCTGCTAGTGTTGTTTCAACAATTGCTTCAAAATTCAAGAGTGATGTGCAAATTGTTTTAGACAAGACAGGAAAAGTTGGAAATCTTAAATCAATTATGAATGTTATGTCTTTAGGTGTTAAAAAAGGCGACGTAATTACTATTAAAATCAATGGAGCAGATGAAGAAGAAGCATACAAAGCTATTTATGATGCTTTTAAATCAAATGAAATTATTTAA", "aa": "MKEFTASIIDPIGLHARPASVVSTIASKFKSDVQIVLDKTGKVGNLKSIMNVMSLGVKKGDVITIKINGADEEEAYKAIYDAFKSNEII", "aa_hexdigest": "3b24c14dcfdb3c64b760de42af70285b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C336", "ncbi_nrp_id": "WP_013354854.1", "uniref100_id": "UniRef100_C4XDX3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C336", "RefSeq:WP_013354854.1", "UniRef:UniRef100_C4XDX3" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX3", "uniref90_id": "UniRef90_A0A3P8MEF8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEF8", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_A0A1H9FWZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A1H9FWZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1H9FWZ8" ], "product": "Phosphocarrier protein HPr" }, "genes": [], "id": "IGIGPGHMMC_1279", "locus": "GCA000148625_01269" }, { "type": "cds", "sequence": "contig_1", "start": 723015, "stop": 723467, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "TTGATCTTAGATGAGCCTGCAGCAAACTTAGATCCAAAAGTTAGAAATGAACTTTATTTTAACTTAAGCAAACTTCACAAACAAGGCAAAACCATTTTTGTTTCTTCTCATATTTTAAGTGAGCTAGAACAATATATTGATAGTCTAACTGTTTTAGATAAAGGTAAAGTTTTATATTCTGGTGACTTAGAAATTTTAAAAAATTCAAACTTTGAAGTATTTATTAAAACTGAACAAATTAATGAAACTATTAAAGCCTTAGAAAAAGCAAAACTAGTTTCATCAAACAAAGAATATCAATGTCGAGAAACGGGTATTGCTGTTGATTTACCTTTCAATAAAATCACAAACATAACAAAAGCATTAATTAGTAGTAATATCGATTTTGAAAACATTGGAAAAAATATTCAAACCTTAAACGAAAAGTTTTTCAAAGTTGAAAAAGCAGAATAA", "aa": "MILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE", "aa_hexdigest": "6b69a40d31f2dd67ac384d48409265bd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 723015, "stop": 723767, "observations": { "mutation": [ 723551 ], "stop": [ 723551 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.49019607843137253, "identity": 0.993, "score": 288.0, "evalue": 3.69e-96, "gene_start": 1, "gene_end": 150, "reference_start": 157, "reference_end": 306, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.992, "score": 478.0, "evalue": 3.22e-173, "description": "Internal stop codon at 723551. Nonsense mutation around 723551.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_1280", "locus": "GCA000148625_01270" }, { "type": "cds", "sequence": "contig_1", "start": 723549, "stop": 723935, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "ATGTCACAAAACGCAATTGAATTTAAAAATGTTACAATGAGTTTTAAAAATTTTAAAGCTTTAGACAATGTGACATTTTCAATCAAAAAAGGTGAATTTCATGGATTTATTGGAGCAAATGGAGCAGGTAAAACTACAAGTTTCCGTTCATTATTGTATTTTTATCCAGAAGTCCAAGGTGAAATTTTAATAGATGGAATTAACTTTAAAAACAAAAAAAGTAGAATCAAAATTGGTTATGTTCCTGAAGTAGCAACATTTCCTAAAAGTCTGAATATTTATGATTACTTAGTTGCTTTAGCTGAATTAAATGGACTTAAAAATGACAAAGCTAAAAAAAGAATTGAAGAATTATTAATCGAGTTAGATGTTAACAAAAAAATATGA", "aa": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDKAKKRIEELLIELDVNKKI", "aa_hexdigest": "0998416f2d0b2cb1b6d6bedd63ef1b4b", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 723246, "stop": 723935, "observations": { "mutation": [ 723551 ], "stop": [ 723551 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.41830065359477125, "identity": 0.992, "score": 251.0, "evalue": 8e-82, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.9912663755458515, "score": 442.0, "evalue": 1.37e-159, "description": "Internal stop codon at 723551. Nonsense mutation around 723551.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_1281", "locus": "GCA000148625_01271" }, { "type": "cds", "sequence": "contig_1", "start": 723978, "stop": 724448, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCATTAATATATAGAAGTACCATTTTTCTCTCAGCTAATGAAGATCCTTCAAAAGCAAAAAATGAAGAAAGAGAATTCAGAAATTTTGTTGAAGATAATTTAACTTTTGACAAAAAAGAAGAATTATTTAAAAAAACTCGTTACTTAAGCTTTGACTATAACTCTATTGCAAAAAAACTTTTCTTTACAATCTTGGTAAAAAATAATTTATATTCTATAGTTGATGCAATGTCAAAAGTTATTATGAATGATTATCGCGAAACTTTAAAAATAAATCAAAATAAAGAATTCTTTAATTCAAGACTTGAAGAAATTTTTGGCAGCATGCTAATTAATAAAGATTTATTAAAAGAAAACAACATTGATTTGAAATTTTACAAAGTACAAAAAGTTAATATATTTCCATCTTACATGCAAATAATTATTTACTTTTTAATGGCTACACCTTTAGCTATTGGCGGATGA", "aa": "MVPLIYRSTIFLSANEDPSKAKNEEREFRNFVEDNLTFDKKEELFKKTRYLSFDYNSIAKKLFFTILVKNNLYSIVDAMSKVIMNDYRETLKINQNKEFFNSRLEEIFGSMLINKDLLKENNIDLKFYKVQKVNIFPSYMQIIIYFLMATPLAIGG", "aa_hexdigest": "9e6cfaac78431b44398c31123c76e476", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18518.32829999999, "isoelectric_point": 8.940878486633302 }, "id": "IGIGPGHMMC_1282", "locus": "GCA000148625_01272" }, { "type": "cds", "sequence": "contig_1", "start": 724692, "stop": 724961, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTATAATGATTTTATTTTAGATGATCCAAAGAGCAACCGCTTTATTCCAATTATGAGTGAAGGAGTTCATAACCAATATTTTTATAAAAGAGATATGGAATCTGTTTTGAATAATTATGATTATGTCACAAATGGAAATCAAAAGATTTATATTATTGATTATGATTTAAGCACAACAGATCGACTTAATCCATATCAAAAACAAGAAACCTTAGAACAAAAAATTACTTATATGGCTCGCAAAAAACACATTATAAAATGA", "aa": "MNFYNDFILDDPKSNRFIPIMSEGVHNQYFYKRDMESVLNNYDYVTNGNQKIYIIDYDLSTTDRLNPYQKQETLEQKITYMARKKHIIK", "aa_hexdigest": "cc82759af5f94e1ac0d146dbe6ee141e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10841.131899999993, "isoelectric_point": 6.715038108825683 }, "id": "IGIGPGHMMC_1283", "locus": "GCA000148625_01273" }, { "type": "cds", "sequence": "contig_1", "start": 725065, "stop": 725505, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTTCATTTTCTGCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAA", "aa": "MFISFSALLSKLAFKFSALLIFDIWWYSSGVEKILIMLKSAGVAINTGKVIPSNALDETFEKIIEINEITKVPIIRASKIKAICKSQILPINLVPFRSSDNPKAAKDIIIAENNALIVALITLIKNALTKEDQNSSVLDTGLEYKT", "aa_hexdigest": "d0431b10a5fc5bc7d7afb84b120e6534", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16096.851799999975, "isoelectric_point": 8.990196800231931 }, "id": "IGIGPGHMMC_1284", "locus": "GCA000148625_01274" }, { "type": "cds", "sequence": "contig_1", "start": 725460, "stop": 725750, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATTATTAAAGTTAAACTTTAAAACAGCATACAAAGACAAGTCAATATTAATTATTAACTTTTTCCTAGTACCGTTTTTACTTTTAATAGGTACCCTTATTATTCTTATTGTATCTCACACAAATTCAGCTCGAGATATAGTTATTTATTATGGAAGCATGTCACCTATTTTAATTATATTGGCCGCAATTATTACGGGTTCTTATATTTTTAAGATGCCTCAAAATGAAGGCTTAACTTATGTTTTATATTCCAAACCAGTATCAAGAACTGAACTGTTTTGA", "aa": "MGKLLKLNFKTAYKDKSILIINFFLVPFLLLIGTLIILIVSHTNSARDIVIYYGSMSPILIILAAIITGSYIFKMPQNEGLTYVLYSKPVSRTELF", "aa_hexdigest": "14d51bf7c06e3b26995d4a4dfff6635b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10844.998199999989, "isoelectric_point": 9.646807289123537 }, "id": "IGIGPGHMMC_1285", "locus": "GCA000148625_01275" }, { "type": "cds", "sequence": "contig_1", "start": 725887, "stop": 726153, "strand": "-", "frame": 2, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354857.1", "SO:0001217", "UniParc:UPI0001A8C339", "UniRef:UniRef100_C4XDX6", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_C4XDX6" ], "nt": "ATGATTACAAAAGAAGAAAAAGCTAAATTAGTTGCTAAGTATGGTGCTAATAAAAAAGACACAGGAAACACATTTGTTCAAATCGCAATTTTAACAGCAGAAATTGAAGATTTGAAAAAACACTTTGCAGCAAATCCTAAAGACAACCACTCACGTCGTGGTTTCTTAGCAAAAATTTCAAAACGTCGTGTTTTATTACAACACCTTAAGTCAAATGACTTAGAAACATACAACAAAGTTTTAGCTGAATTAAACTTAAGAAAATAG", "aa": "MITKEEKAKLVAKYGANKKDTGNTFVQIAILTAEIEDLKKHFAANPKDNHSRRGFLAKISKRRVLLQHLKSNDLETYNKVLAELNLRK", "aa_hexdigest": "ffd16c098c72fee1d0f9208017cda9d8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C339", "ncbi_nrp_id": "WP_013354857.1", "uniref100_id": "UniRef100_C4XDX6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C339", "RefSeq:WP_013354857.1", "UniRef:UniRef100_C4XDX6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX6", "uniref90_id": "UniRef90_C4XDX6" }, "psc": { "uniref90_id": "UniRef90_C4XDX6", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "IGIGPGHMMC_1286", "locus": "GCA000148625_01276" }, { "type": "cds", "sequence": "contig_1", "start": 726475, "stop": 726894, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_013354858.1", "SO:0001217", "UniParc:UPI0001A8C33A", "UniRef:UniRef100_C4XDX7", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A5IZ35" ], "nt": "ATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAA", "aa": "MPTTNQLVTSGRVSKVRKQKAPALNLSFNTLTKEGRKLPAPFKRGVCTRVATMTPKKPNSAMRKYARVKLSNGMEVTAYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTQDLAGVNNRKQGRSLYGTKKEKAAK", "aa_hexdigest": "7688e7a0833844e3d3b9aa9f6605bfa5", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C33A", "ncbi_nrp_id": "WP_013354858.1", "uniref100_id": "UniRef100_C4XDX7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C33A", "RefSeq:WP_013354858.1", "UniRef:UniRef100_C4XDX7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX7", "uniref90_id": "UniRef90_A5IZ35" }, "psc": { "uniref90_id": "UniRef90_A5IZ35", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "IGIGPGHMMC_1287", "locus": "GCA000148625_01277" }, { "type": "cds", "sequence": "contig_1", "start": 726921, "stop": 727229, "strand": "+", "frame": 3, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "nt": "ATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGA", "aa": "MSRKHSAPIREVLADPVFNSVLVTKLINTIMLDGKKSIAQDILYSAFNIIKEKTNKEPMEVFLAAVENVTPQLEVRTRRIGGTNYQVPTEVSDRRKQTLSLR", "aa_hexdigest": "a0f42759ce6eb781d4da3fa6591175b7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A2W4JVM3", "query_cov": 0.9901960784313726, "subject_cov": 0.8145161290322581, "identity": 0.505, "score": 111.0, "evalue": 1.39e-29, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A920EYB2", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920EYB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "IGIGPGHMMC_1288", "locus": "GCA000148625_01278" }, { "type": "cds", "sequence": "contig_1", "start": 727233, "stop": 727388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "GTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGA", "aa": "MQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANRAFAHFR", "aa_hexdigest": "338a9f9397997fad27c6d53d83c43be9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8431372549019608, "subject_cov": 0.86, "identity": 0.7909999999999999, "score": 73.9, "evalue": 1.41e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 1.2663252935181254e-18, "score": 63.072261810302734, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5905.67, "isoelectric_point": 9.983783531188966 }, "id": "IGIGPGHMMC_1289", "locus": "GCA000148625_01279" }, { "type": "cds", "sequence": "contig_1", "start": 727403, "stop": 727558, "strand": "+", "frame": 2, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGGCAAGACAATATAAATTAGAAGATTACCGTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGA", "aa": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMD", "aa_hexdigest": "4ef82af48139a850154250cb626782c1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.725, "score": 82.8, "evalue": 4.66e-20, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 727403, "stop": 727623, "observations": { "deletion": [ 727607 ], "mutation": [ 727556 ], "stop": [ 727556 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 1.0, "score": 107.0, "evalue": 9.59e-30, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.9863013698630136, "score": 137.0, "evalue": 3.92e-44, "description": "Internal stop codon at 727556. Frameshift due to deletion around 727607. Nonsense mutation around 727556.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "IGIGPGHMMC_1290", "locus": "GCA000148625_01280" }, { "type": "cds", "sequence": "contig_1", "start": 727811, "stop": 728926, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "nt": "ATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCAGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGA", "aa": "MDKAGADFFAAVKSVKERLNGNAVAIQIPIGAEAHFKGLVDLVEMKAYEYDGKPEENAKEIVIPSDLAELAKTKRQELIEAVASYDDDFMMQVLEGVEPDLATLKKVIRKATLTAEFYPAVCGTSFKNKGVKKMIDAVVDYLPSPLDIPATKAHLDDKEVAIPATDDHDFIGLAFKVMTDPYVGSLTFVRGYAGILNKGSYVYNSTKDVKERIGRLIQMHANSRQEIDECYAGDIVAAVGLKSTTTGDTLVAEKSPKYILEKMIFPEPVISQALEPESKDAMEKLALGLQKLAAEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRLKREHGVKAKVGAPQVSYRETITKSAEVEGKHIKQSGGKGQYGHV", "aa_hexdigest": "7bf6df4d164d87d183a46180eabbe2f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00255A9B97", "query_cov": 0.9892183288409704, "subject_cov": 1.0027322404371584, "identity": 0.504, "score": 356.0, "evalue": 1.78e-118, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A379SQ57" }, "pscc": { "uniref50_id": "UniRef50_A0A379SQ57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "product": "Elongation factor G" }, "genes": [], "id": "IGIGPGHMMC_1291", "locus": "GCA000148625_01281" }, { "type": "cds", "sequence": "contig_1", "start": 729038, "stop": 729493, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "nt": "ATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAA", "aa": "MAGGILAGYPMIDIKATLFDGSYHDVDSSELAYKIAASKALTKAKDLLGTVLLEPIMDVAVVIPEDYFGDVMGDLTRRRGQIEDNETRNDGAHIIRSLVPLKEMFGYATDLRSMTAGRGTYQMQFHHYEKCPKNIADEIIKRRNIQVKDED", "aa_hexdigest": "7c133eebb2053ee313796d038b4a2d0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PQ45", "query_cov": 1.0, "subject_cov": 0.8628571428571429, "identity": 0.7809999999999999, "score": 246.0, "evalue": 1.33e-81, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A3B0PQ45" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "IGIGPGHMMC_1292", "locus": "GCA000148625_01282" }, { "type": "cds", "sequence": "contig_1", "start": 730000, "stop": 730137, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1", "UniRef:UniRef90_C4XDY1" ], "nt": "ATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGA", "aa": "MYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIA", "aa_hexdigest": "d889d697825ad7dea3f2ec514fd6c993", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 729901, "stop": 730236, "observations": { "mutation": [ 729952, 730135, 730147, 730192 ], "stop": [ 729952, 730135, 730147, 730192 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY1", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 1.0, "score": 82.0, "evalue": 3.33e-19, "gene_start": 1, "gene_end": 45, "reference_start": 34, "reference_end": 78, "reference_sequence": "MKLVLEEYILTGSILSNWDKIQHSNVDRIGMLNMYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIAWKDGWPSLINNNSNALVQIWFDYYINNIMNLDE" }, "paralog": false, "identity": 0.954954954954955, "score": 201.0, "evalue": 1.08e-68, "description": "Internal stop codon at 729952, 730135, 730147, 730192. Nonsense mutation around 729952, 730135, 730147, 730192.", "psc": { "uniref90_id": "UniRef90_C4XDY1", "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY1", "UniRef:UniRef50_C4XDY1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5066.796700000002, "isoelectric_point": 8.064301872253417 }, "id": "IGIGPGHMMC_1293", "locus": "GCA000148625_01283" }, { "type": "cds", "sequence": "contig_1", "start": 730387, "stop": 730623, "strand": "+", "frame": 1, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707", "UniRef:UniRef90_UPI000F03D707" ], "nt": "TTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGA", "aa": "MNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFN", "aa_hexdigest": "7361005b9c6b6c11cf413be80a4df78c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 730360, "stop": 730863, "observations": { "mutation": [ 730621, 730834 ], "stop": [ 730621, 730834 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F03D707", "query_cov": 1.0, "subject_cov": 0.46706586826347307, "identity": 0.987, "score": 154.0, "evalue": 1.25e-46, "gene_start": 1, "gene_end": 78, "reference_start": 10, "reference_end": 87, "reference_sequence": "MDTRTIDDYLNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFNWNILHYRDKWSNEVDCILQLKNGDFAAIKIKLSNSYEEQTATGLLRFKKETEEEQKLAKINREKMYKKVPKWLIIISGTA" }, "paralog": false, "identity": 0.9760479041916168, "score": 318.0, "evalue": 1.02e-113, "description": "Internal stop codon at 730621, 730834. Nonsense mutation around 730621, 730834.", "psc": { "uniref90_id": "UniRef90_UPI000F03D707", "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F03D707", "UniRef:UniRef50_UPI000F03D707" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707" ], "product": "DUF4143 domain-containing protein" } }, "id": "IGIGPGHMMC_1294", "locus": "GCA000148625_01284" }, { "type": "cds", "sequence": "contig_1", "start": 730969, "stop": 731340, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3", "UniRef:UniRef90_C4XDY3" ], "nt": "ATGGCTTTTATCTTTTATTTGTGCATCTTTATTATGCCAATTTACAAAGGTTTAGAGTATAAATATGACAATAATATAATGAAGCAAACTAAAGATTTAGCGGCGACTGCAGCAGCATTTTGTATGATAGTTGTAATTGTTTATTTAAGTATTACAGTTGAATCATTTCTAATCTTGAAAAAGTTAATAAAATTCCCTGAAGTAACAGCTGAAATTTATTATATGACTTTTCTTTTTCATAATGATCGTACAATGTTCAAAGATTATTTTGAAAGTGAAAAAATTAATCTAGATCCAAGAGTTAAAAAAGAAATTATGGACATAGTTTCTAATGAATCAAAAGCCAAAGGAATAAAGATCATTTTTAGATAA", "aa": "MAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKDYFESEKINLDPRVKKEIMDIVSNESKAKGIKIIFR", "aa_hexdigest": "c0448fb5163b9bd35ba9a9c1dda672f0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 730969, "stop": 731640, "observations": { "mutation": [ 731397, 731472, 731583 ], "stop": [ 731397, 731472, 731583 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY3", "query_cov": 1.0, "subject_cov": 0.5394736842105263, "identity": 0.9840000000000001, "score": 234.0, "evalue": 1.29e-76, "gene_start": 1, "gene_end": 123, "reference_start": 106, "reference_end": 228, "reference_sequence": "MLETFNQEAQKYHTEEVKLRRDLSWSSLFAFISLLVAFTITVTFLSLALKNYDYYLSGLNYWYNFFVYIATSSIISLITLILACYFWIKFDRKGVSKYNIIASCLMAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKNYFESEKINLDPRIKKEIMDIVSNESKAKGIKIIFR" }, "paralog": false, "identity": 0.9775784753363229, "score": 425.0, "evalue": 3.41e-154, "description": "Internal stop codon at 731397, 731472, 731583. Nonsense mutation around 731397, 731472, 731583.", "psc": { "uniref90_id": "UniRef90_C4XDY3", "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY3", "UniRef:UniRef50_C4XDY3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14541.253599999987, "isoelectric_point": 8.783704566955567 }, "id": "IGIGPGHMMC_1295", "locus": "GCA000148625_01285" }, { "type": "cds", "sequence": "contig_1", "start": 731924, "stop": 733018, "strand": "+", "frame": 2, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "EC:2.5.1.6", "GO:0004478", "GO:0005524", "GO:0006556", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P72871", "UniRef:UniRef90_C4XDY4" ], "nt": "ATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGACCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGA", "aa": "MNLNKQILFTSESVGQGHPDKICDQISDAILDEYLRKDPYSRCAIETMATGNMLIIAGEASSKANVDHKQIAVNVLKKAGQYSDHLRFHLDVKKQSTDISQGVIRANEEIGAGDQGIMFGYATNETRNYMPLAITLAHEIVKRADKLRERGLFKYAKSDMKSQVTLDYTDSKRIKVDTILFSCQHEENFNEKEFKEYIVKNIFEPVLQDYSLKLPVKILINPTGRFVIGGPIGDTGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSAAYACRYVTKNLVAAGCADKIELQVSYAIGVAEPVSILVETFGTEKIAKDKIVSIVHKLFDLRPKHIIESLNLRRPIYQQTSYFGHFGREDLDLP", "aa_hexdigest": "13f77e9da3324e0f50c0b51c052ed3c0", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_C4XDY4", "query_cov": 1.0, "subject_cov": 0.9405684754521964, "identity": 1.0, "score": 715.0, "evalue": 5.39e-260, "valid": true, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P72871", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_P72871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P72871" ], "product": "S-adenosylmethionine synthase" }, "genes": [ "metK" ], "id": "IGIGPGHMMC_1296", "locus": "GCA000148625_01286" }, { "type": "cds", "sequence": "contig_1", "start": 733142, "stop": 733258, "strand": "+", "frame": 2, "gene": null, "product": "MarR-family transcription repressor", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5", "UniRef:UniRef50_C4XDY5", "UniRef:UniRef90_C4XDY5" ], "nt": "ATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAA", "aa": "MRKIVRAKNKERAAKRLHKLQLEEARDKRRAAREAEAK", "aa_hexdigest": "aa1de1c111f157ffb37add61c45210a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C342", "uniref100_id": "UniRef100_C4XDY5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY5", "uniref90_id": "UniRef90_C4XDY5" }, "psc": { "uniref90_id": "UniRef90_C4XDY5", "product": "MarR-family transcription repressor", "uniref50_id": "UniRef50_C4XDY5" }, "pscc": { "uniref50_id": "UniRef50_C4XDY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY5" ], "product": "MarR-family transcription repressor" }, "genes": [], "id": "IGIGPGHMMC_1297", "locus": "GCA000148625_01287" }, { "type": "cds", "sequence": "contig_1", "start": 733568, "stop": 733939, "strand": "-", "frame": 1, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354867.1", "SO:0001217", "UniParc:UPI0001A8C361", "UniRef:UniRef100_C4XDY6", "UniRef:UniRef50_Q5ZZS0", "UniRef:UniRef90_A0A3P8K8J4" ], "nt": "ATGGCAAAATTAACAAAAGAATCATTCATTTCATCATTAAAAGAAATGTCAATTAAAGAAGTTATGGAATTAGTTGAAGCAATGAAAGAAGAATTTGGAATTGACCCATCAGCTGTTGCTGTTGCTGCTGCTCCTGCTGCAGCTGCAGCTGCTGAAGGTCCTTCAGTAGTTACAGTTTCAATTACTTCAGACAATGGTAAAAAATTACCTATTGTTAAAGCAGTTAAAGAATTATTAAACTTAGCTTTAATGGATGCAAACAAATTAGTTTCAAACCTTCCAGCTGTTGTTAAAGAAAACATCAAACCAGATGAAGCAGAAGCTATCAAAGCTAAACTTGTTGAAGCTGGTGCTACAGTTGAAGTTAAATAA", "aa": "MAKLTKESFISSLKEMSIKEVMELVEAMKEEFGIDPSAVAVAAAPAAAAAAEGPSVVTVSITSDNGKKLPIVKAVKELLNLALMDANKLVSNLPAVVKENIKPDEAEAIKAKLVEAGATVEVK", "aa_hexdigest": "ef67351208754f1f940e309ae7ffc49c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C361", "ncbi_nrp_id": "WP_013354867.1", "uniref100_id": "UniRef100_C4XDY6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C361", "RefSeq:WP_013354867.1", "UniRef:UniRef100_C4XDY6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY6", "uniref90_id": "UniRef90_A0A3P8K8J4" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8J4", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_Q5ZZS0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZS0" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "IGIGPGHMMC_1298", "locus": "GCA000148625_01288" }, { "type": "cds", "sequence": "contig_1", "start": 733961, "stop": 734470, "strand": "-", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013354868.1", "SO:0001217", "UniParc:UPI0001E32F67", "UniRef:UniRef100_UPI0001E32F67", "UniRef:UniRef50_Q98QS1", "UniRef:UniRef90_A0A3P8MDE2" ], "nt": "ATGCAAAAAAGACATGAATCTGCTTTTAGATTAGTTAAAAAAGATACAGTTAAAGAAATTCAAAATAAACTTGAATCTTCACAAGCTCTTGTTATAGCTGAATATCGTGGATTAAGTGTTGCTGAATTGACTTCATTACGTCAAGAAGCTAAAAAAGAAGGTATTGACATTAAAGTTTACAAAAACCGTCTTTTCAAAATAGCTGCTAAAAATCATGGCATTAATGATTTAGATCAACACTTAGTAGGACCAAACATTTTTGCTTTTGGTACAGCCGATGCTCTAATTGCTGCAAAAGTTTTAGTTAAATTCTCTAAAGAAAACAAACTTTTGGTTCCTAAAGCTGGTATTTTTGAAGGCAAAGTTATCGATGCTAAAGGTGTTCAAGAAGTTGCTTCATTACCAAACTATGAAGAAGCTCTTACAATTCTTGCTCGTTCACTTATGTCACCATTACAACAACTCTCTCTTTCTCTAAAAATGTTTAGTGAAAAAGAAGAAAACAAATAA", "aa": "MQKRHESAFRLVKKDTVKEIQNKLESSQALVIAEYRGLSVAELTSLRQEAKKEGIDIKVYKNRLFKIAAKNHGINDLDQHLVGPNIFAFGTADALIAAKVLVKFSKENKLLVPKAGIFEGKVIDAKGVQEVASLPNYEEALTILARSLMSPLQQLSLSLKMFSEKEENK", "aa_hexdigest": "5f4919ee55eee164880586368caf22ec", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001E32F67", "ncbi_nrp_id": "WP_013354868.1", "uniref100_id": "UniRef100_UPI0001E32F67", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F67", "RefSeq:WP_013354868.1", "UniRef:UniRef100_UPI0001E32F67" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F67", "uniref90_id": "UniRef90_A0A3P8MDE2" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDE2", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_Q98QS1", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QS1" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "IGIGPGHMMC_1299", "locus": "GCA000148625_01289" }, { "type": "cds", "sequence": "contig_1", "start": 734877, "stop": 735428, "strand": "-", "frame": 3, "gene": null, "product": "Hemolysin C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8", "UniRef:UniRef90_C4XDY8" ], "nt": "ATGGCTCAAAATGCTTTAGACTTAGATAGTACCAAAGTTAAAAAACACTACATAAGATTAAAAGATGTATCTATAATTGATGCTAATGCTAATATTCAAGAAGCATTTGAAATTTTTAAAGATACAAACTATAGCCGTATCCCTGTTAAAAAAGATGAAAATTTAATTGGCATTTTACACTTAAAAGATGTCTTCTTTTTACAAAAAGGTAAAGTTATAAACTATTTTAAAACTGTGCCTACTATTAGTGCTAATGCTTCTCTTTCTTCGGCTCTAGAAAAAATGAGATACCAAAGAGCTCAAATGGCTTTTGTTACACCAAATAACAACTCAAGTGAAATAATTGGAATTATTACAATTGAAGACATTATTGAAGAAATTGTTGGCGAAATTTATGATGAATTTGATGATGAAGAATTAAAAGACATTTTTGAAATTAGTCTTGAACACTTCCATGTCTCAGCTTCTTTAACAATGAAAGAATTAATTAAAAAACTAGAAATAGAATTAGATCTTGAAGAAGAAGAAAATGAATTAACACTTCATAAATGA", "aa": "MAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHK", "aa_hexdigest": "89d118c33660164ee6c9df534fe2acf8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 734694, "stop": 735728, "observations": { "mutation": [ 734879, 735554 ], "stop": [ 734879, 735554 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY8", "query_cov": 1.0, "subject_cov": 0.4255813953488372, "identity": 1.0, "score": 346.0, "evalue": 1.33e-116, "gene_start": 1, "gene_end": 183, "reference_start": 187, "reference_end": 369, "reference_sequence": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTSWLLSTYLGKKFESYNVLISTAVMTPIIVLFGEITPKLIAKSKPEATVKVFCYLLIILYYLFWPITYPISKIGKKIYITNTENDVKNIIDIAQNEGVLEANESIMAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHKWLEKRLGHKLYKNTKYEYNDVYFKVIDLGTNRFKNVRVDIELGNKADILNTDETLTDNEKN" }, "paralog": false, "identity": 0.9941860465116279, "score": 664.0, "evalue": 2.28e-244, "description": "Internal stop codon at 734879, 735554. Nonsense mutation around 734879, 735554.", "psc": { "uniref90_id": "UniRef90_C4XDY8", "product": "Hemolysin C", "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY8", "UniRef:UniRef50_C4XDY8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8" ], "product": "Hemolysin C" } }, "id": "IGIGPGHMMC_1300", "locus": "GCA000148625_01290" }, { "type": "cds", "sequence": "contig_1", "start": 735735, "stop": 735986, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGACCCGAAACTGAAATTAGGATTAAGCATATTCGGACTAGTTATTTTGTTAATAGCTAGCGGAATTTTTAGTGCCAGTGAAACTGCTTATACATCCTTAAATGTTGGCAAAATTGAAACAATGGTTGGCAATAAAGAATTTGGCCACAAAATAATCAAAAAACAACATACATTTTTCAACCAAACTTTAAGTACTATTTTAATATGCAACAATATAGTTAATATTGCTGCTTCGGCCTTAACATCCTGA", "aa": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTS", "aa_hexdigest": "4539d5b404e9c43d6236a0c757912a45", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 69, "aa_cov": 0.8313253012048193, "hmm_cov": 1.0, "evalue": 1.5756019303703824e-18, "score": 63.04677200317383, "start": 14, "stop": 82 } ], "seq_stats": { "molecular_weight": 8940.45019999999, "isoelectric_point": 9.302997398376462 }, "id": "IGIGPGHMMC_1301", "locus": "GCA000148625_01291" }, { "type": "cds", "sequence": "contig_1", "start": 736073, "stop": 736423, "strand": "-", "frame": 1, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "nt": "TTGATTTCATCAGACTCAAATAGAAGAAATGAAAGTCGGCAAAATGAAATAGCTAAAATTAGCCGTTCATTAAAAACATTAGCCTTAGAATTAAATGTTCCAATTATTGCACTAAGCCAACTTTCCCGTGAAGTTGAAAAACGTGAAGACAAAAGACCTATTATGTCTGACTTACGTGAATCAGGTAACATTGAACAAGATGCCGATATTGTTATGTTCTTATATCGTGAAAATTATTATAAAAAACAAGTCTCACAAGAAACCAAAGATCTCTTTGGAGATATTGGAGAAAAAATAGATATCATTATTGCAAAACACAGAAATGGACCAACAGGAATTTTTCCATTATGA", "aa": "MISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPL", "aa_hexdigest": "454095d752080a09bbd74ecb7cd29104", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001AC6C2CE", "query_cov": 0.9913793103448276, "subject_cov": 0.8455882352941176, "identity": 0.565, "score": 111.0, "evalue": 3.35e-29, "valid": false, "product": "DnaB-like helicase C-terminal domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A7W1AXE1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A7W1AXE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "IGIGPGHMMC_1302", "locus": "GCA000148625_01292" }, { "type": "cds", "sequence": "contig_1", "start": 736484, "stop": 737413, "strand": "-", "frame": 1, "gene": "dnaB", "product": "replicative DNA helicase", "db_xrefs": [ "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260", "SO:0001217", "UniRef:UniRef50_C4XDY9", "UniRef:UniRef90_C4XDY9" ], "nt": "ATGCCAAATAATAATCCAAGTAATGCACCAATAAATACATCTCGTCATATTAACTCTAGATATGAGGAGTCTCTTTTAGGCTTAATCTTAACAGACAACAAAAACGCACCTAAAATTGTACCTTACTTACTTGAAGAAGACTTTGCGATTTTAGAAAATCAAAGACTTTTTGCTGTTTTTAAAGAACTATTTGAAAACAACATTTCGATTAATGAAGAAAACATTTTTACTATTGCTAAAAAAAGAAACTTTGTTCAAATAACACCTTTATATTTATCTAAACTTTATCATACTGCTGGTTTCTCATCTAATATTCAAACTTACTTAGAAGAATTAGTTAAGTTAACAAAATTACGTTTAATTGAATCACGTTTAGCTTTAGTTCAAAGAAGACTTGATGTTGAAAAAGCAATCAATGAAAAAGATGTTATTTCAGATTTACAAAACCTATTACTTGACATTGATCGTAGTCAAGTTAGTGCAGACTTTTTAACAGCCAAAGAAGTTAGCGATGAATACTTTAAAGATTTAGAAAATCGCCACTCAAGAGATCTAAATGAATTAAATGGACTAGCAACAGGTTACGACAATTTAGACCAAATTACTCAAGGTCTACATGGTGGTGAATTAATTATTATTGCTGCTCGTCCAGCAATGGGTAAAACTGCTTTTGCTCTTAATATTGCTTCAAATGTTGCTAAAAGAAAAAACAGAAGAGCTGTCTTCTTTACGCTTGAAATGTCATCAAACCAACTTATGGGTAGAATTTATAGCTTGAACACAAATGTGCCACTTCAAAAACTAAAACAACCTCAAACAATTACAGATAATGAATTTCTTTTAATCAACAGTTTAAAACAAGATGTAATTGACAAAATGAACTTATTTATAGATGAATCAGTAAATAATGAATTAAATACACTGCTTTGA", "aa": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFAVFKELFENNISINEENIFTIAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLL", "aa_hexdigest": "6d5d3f7fa5ba678fa3be1ff68abafde6", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 736181, "stop": 737413, "observations": { "mutation": [ 736486 ], "stop": [ 736486 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY9", "query_cov": 1.0, "subject_cov": 0.6518987341772152, "identity": 0.9940000000000001, "score": 585.0, "evalue": 6.06e-208, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFSVFKELFENNISINEENIFTVAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLLWKCRRLHKVSPIDLIVIDYLQLISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPLWFKMSCGRFINFENEQIAVSSDDDEEEE" }, "paralog": false, "identity": 0.9926829268292683, "score": 785.0, "evalue": 3.72e-289, "description": "Internal stop codon at 736486. Nonsense mutation around 736486.", "psc": { "uniref90_id": "UniRef90_C4XDY9", "gene": "dnaB", "product": "replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDY9", "go_ids": [ "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY9", "EC:5.6.2.3", "UniRef:UniRef50_C4XDY9", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY9" ], "product": "Replicative DNA helicase" } }, "id": "IGIGPGHMMC_1303", "locus": "GCA000148625_01293" }, { "type": "cds", "sequence": "contig_1", "start": 737403, "stop": 737858, "strand": "-", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02939", "RefSeq:WP_013354871.1", "SO:0001217", "UniParc:UPI0001E32F69", "UniRef:UniRef100_UPI0001E32F69", "UniRef:UniRef50_Q4A5Y3", "UniRef:UniRef90_C4XDZ0" ], "nt": "ATGAAAGTAATATTGATTAAAGATTGTGATAAAGGTAAAGCCAATATAATTATTGAAGTTTCAGCTGGTTATGGTAATAACTTCTTAATTGCAAAAGGTTTTGCAGTTGTCTATAATGACAAGACTAAAAAAGAGCTTGAACAAAGACTTAGTGAGCTTACTAAAAATGAAATGGAAAAACGTTCAGAAGCTCTTGAACTTAAAGATCAAATTGAAAAATTAACTTTAAAATATACTTTAGATGCACATATTGACAATAATGGAAATTTAATTGCTCATGGTGCTGTATCAACCAAAGATATTGTTAAAGATTTAGTTCAAAATGGCTATAAATTAGATAAATACGCAGTTCAAAAAGTTCATTTAGTATCTAATGGATTGCATGATATAGATGTTATTTTATATAAAGATATCATTGCAAAATTGAAAGTAGAAGTGATAATTAATGCCAAATAA", "aa": "MKVILIKDCDKGKANIIIEVSAGYGNNFLIAKGFAVVYNDKTKKELEQRLSELTKNEMEKRSEALELKDQIEKLTLKYTLDAHIDNNGNLIAHGAVSTKDIVKDLVQNGYKLDKYAVQKVHLVSNGLHDIDVILYKDIIAKLKVEVIINAK", "aa_hexdigest": "0217fc66e06b5739a4731c19a071dde2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32F69", "ncbi_nrp_id": "WP_013354871.1", "uniref100_id": "UniRef100_UPI0001E32F69", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F69", "RefSeq:WP_013354871.1", "UniRef:UniRef100_UPI0001E32F69" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F69", "uniref90_id": "UniRef90_C4XDZ0" }, "psc": { "uniref90_id": "UniRef90_C4XDZ0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_Q4A5Y3", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5Y3" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "IGIGPGHMMC_1304", "locus": "GCA000148625_01294" }, { "type": "cds", "sequence": "contig_1", "start": 738253, "stop": 738753, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "TTGGATGCAATCGGTTCAGCTTTAGGAGTTTATGCTATAGCTAAAACATTTGATAAAGAAGCTTACATTTGCACAATGACTCAAGATGTTACAACTAAAAAAGCAATTGCTAAATACTTTGGTGAAGATAATTCAATTTTCATTAAAGCTCATCAAGCTAACACATTGACTGATGAAAATACACTTGTATTTTTAGTTGATAACTCAGCTCCAAATAGGACTGATAATCCAAAATGTGTTGAAAAAGCTAAAAACACAAATATCTTTATTTTAGACCACCATAGATTAAATTCTTCAGTTGACTTTTGTTCAAAAATTAATCGCCACATTGAGCCTTCAGCTTCAAGTGCTAGTGAAATAGTAACTGAATTAATGATGTTTTTAGAAAAGCAAGTTAAAGTTAAAAAGAACATTGCTCAAATGTTATTAAATGGTATTTATCTTGACACCTTACAATTTCAAAAACATGTTACTTCAAGAACATTTGAAGCAGCAAGTTGA", "aa": "MDAIGSALGVYAIAKTFDKEAYICTMTQDVTTKKAIAKYFGEDNSIFIKAHQANTLTDENTLVFLVDNSAPNRTDNPKCVEKAKNTNIFILDHHRLNSSVDFCSKINRHIEPSASSASEIVTELMMFLEKQVKVKKNIAQMLLNGIYLDTLQFQKHVTSRTFEAAS", "aa_hexdigest": "365d5388e9140d0525ce1cb025e471a5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 151, "aa_cov": 0.8795180722891566, "hmm_cov": 0.7549668874172185, "evalue": 1.9849174936416814e-19, "score": 66.1521224975586, "start": 1, "stop": 146 } ], "seq_stats": { "molecular_weight": 18582.040599999993, "isoelectric_point": 6.955978584289551 }, "id": "IGIGPGHMMC_1305", "locus": "GCA000148625_01295" }, { "type": "cds", "sequence": "contig_1", "start": 738820, "stop": 738975, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "ATGGAACAAGCTAAAAATGCTTTATTACAAGCTCAAAGTCGTGGTGGTGACCAAGTAGCTATTTTCTCAAGAAATAATTTACCTCGTTACTTTGGTTCAACAAGTGAAATCTTGCCAAATATCAACCGTGCAAGAATCAAAGCACTTGCTAATTGA", "aa": "MEQAKNALLQAQSRGGDQVAIFSRNNLPRYFGSTSEILPNINRARIKALAN", "aa_hexdigest": "e3af2c8c559a26432e1e324b3da8177b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 26, "aa_cov": 0.5098039215686274, "hmm_cov": 1.0, "evalue": 1.456453010235842e-09, "score": 34.28099060058594, "start": 2, "stop": 27 } ], "seq_stats": { "molecular_weight": 5644.3451, "isoelectric_point": 10.877960014343262 }, "id": "IGIGPGHMMC_1306", "locus": "GCA000148625_01296" }, { "type": "cds", "sequence": "contig_1", "start": 738982, "stop": 739176, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "GTGTGTGATTACAACTTAGTTTATCGTCAATATAATACAAACGGTAAATTTCTTATTGTAACTAACAAGCAAGCTTTAGATAAAATGAGTCAAGTAAACTTTAAGTTTTTTGATGATTTACATAAAACACTAAAAAGTCAAAACATTATTATTTCAGTTTCTGCAGGTTTTGCATATGGAACAAAAAACTTATGA", "aa": "MCDYNLVYRQYNTNGKFLIVTNKQALDKMSQVNFKFFDDLHKTLKSQNIIISVSAGFAYGTKNL", "aa_hexdigest": "68e3dcb4fbc18fedaea0f8016eeccf5f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 62, "aa_cov": 0.96875, "hmm_cov": 0.967741935483871, "evalue": 2.8564507174139085e-18, "score": 62.42011642456055, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 7399.440099999999, "isoelectric_point": 9.41704196929932 }, "id": "IGIGPGHMMC_1307", "locus": "GCA000148625_01297" }, { "type": "cds", "sequence": "contig_1", "start": 739513, "stop": 739809, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAACAAAAAATAATTTTATATTCTTTAATAGCAGTTGCTGGCGTTTTCCTAGCTGTAGGGTCAGTAATTTTGTATTTTACTCGTTCAGATATTTATAGCAGCATTTTTGGAATTTTTGGCATATGTCTTTCATTAATAATCGTTGGATTATTTGGAATTTTATCTGTTAAAAGTTTTTTATCAACTCAAGCTATAGTAAAAAAATCTTTTAACAGTTTCATTGATGAAATCATTAGTCATAATGCAATTGGAGTGCTTATTTTTGACAGTGAAGGCCAAATTCTTTGA", "aa": "MNLKQKIILYSLIAVAGVFLAVGSVILYFTRSDIYSSIFGIFGICLSLIIVGLFGILSVKSFLSTQAIVKKSFNSFIDEIISHNAIGVLIFDSEGQIL", "aa_hexdigest": "7b6b56416ddb61cfdc0263c92ea312e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10664.598699999986, "isoelectric_point": 7.878761863708497 }, "id": "IGIGPGHMMC_1308", "locus": "GCA000148625_01298" }, { "type": "cds", "sequence": "contig_1", "start": 740055, "stop": 740243, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATATTGACCACAATAATAATGATTCTAAAAAACTTATAAATTCCTTTAATGAAATGCTTGATTTAAATATTGAAAACAAACTCCAATTTTTTATAGATGAATTTAGAAAAACAACAGATGATAAAATACTAAAATTTGTAGTTATCAAAAAAATGAGTAAGAAAAAAATTCTTAAAAATTGA", "aa": "MANIDHNNNDSKKLINSFNEMLDLNIENKLQFFIDEFRKTTDDKILKFVVIKKMSKKKILKN", "aa_hexdigest": "93a2e495e1640e9059b2126364f0fb12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7415.6123, "isoelectric_point": 9.52289905548096 }, "id": "IGIGPGHMMC_1309", "locus": "GCA000148625_01299" }, { "type": "cds", "sequence": "contig_1", "start": 740550, "stop": 740711, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACATAATGCTTTATCTAGTTAATAATAAATTAACCACTTACAATTCACAGTATATTTTTAGAGAAAAAGACAAACGCTATATCTTTGAAAAAAGCTCCCTAAAAGAAATATTAAATAAAAATTTAAATGCAAACACAGGTCAAATTATAACCTGA", "aa": "MKNIMLYLVNNKLTTYNSQYIFREKDKRYIFEKSSLKEILNKNLNANTGQIIT", "aa_hexdigest": "27cffad36fb3cd110d09ec888cf5ebb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6359.332699999998, "isoelectric_point": 9.755307579040526 }, "id": "IGIGPGHMMC_1310", "locus": "GCA000148625_01300" }, { "type": "cds", "sequence": "contig_1", "start": 741867, "stop": 742046, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTTAAATTGGAAAACGCGATAGCAAAAAAAGTGAGCGTTTTTGGTTCAATTAAAAGACTCACTAACAAAGAAAAATTCCAAATAATAATTGAACTAAGGCAAGAATTTAAAAAGTTATTTTTCTATTAA", "aa": "MEDKDLLIKQLKEENKILKLENAIAKKVSVFGSIKRLTNKEKFQIIIELRQEFKKLFFY", "aa_hexdigest": "823bd960e6c8af039b549940ac6ef9d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7129.452299999997, "isoelectric_point": 9.646807289123537 }, "id": "IGIGPGHMMC_1311", "locus": "GCA000148625_01301" }, { "type": "cds", "sequence": "contig_1", "start": 742319, "stop": 742582, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354876.1", "SO:0001217", "UniParc:UPI0001E32F6E", "UniRef:UniRef100_UPI0001E32F6E", "UniRef:UniRef50_UPI0001E32F6E", "UniRef:UniRef90_UPI0001E32F6E" ], "nt": "ATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGAAAAAGCTATTTCAAATTTATTTATCGATATTAAGAAATCAGATAAATTTTATGATGATGAATTGAAGCTTAAAATTTATGCTATTAAAAATGATGGTAGTGGCAAAGTATTCAGAACCAAAATTTACTAA", "aa": "MIQKFRPELVGRFKDISFLDKFNAKQVKALINKKIKEDEINIYCEKAISNLFIDIKKSDKFYDDELKLKIYAIKNDGSGKVFRTKIY", "aa_hexdigest": "cb31704f648eb3fa4506807ebdceb6d3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F6E", "ncbi_nrp_id": "WP_013354876.1", "uniref100_id": "UniRef100_UPI0001E32F6E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F6E", "RefSeq:WP_013354876.1", "UniRef:UniRef100_UPI0001E32F6E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F6E", "uniref90_id": "UniRef90_UPI0001E32F6E" }, "psc": { "uniref90_id": "UniRef90_UPI0001E32F6E", "uniref50_id": "UniRef50_UPI0001E32F6E" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F6E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F6E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10312.01399999999, "isoelectric_point": 9.520126914978025 }, "id": "IGIGPGHMMC_1312", "locus": "GCA000148625_01302" }, { "type": "cds", "sequence": "contig_1", "start": 742655, "stop": 742924, "strand": "+", "frame": 2, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "nt": "TTGTTGTATAATACAAAAACTATGGATCAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGA", "aa": "MLYNTKTMDQNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLV", "aa_hexdigest": "d1aa701c23c2a49332ae04a3e21d5dd1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0022A714CF", "query_cov": 0.8651685393258427, "subject_cov": 0.8850574712643678, "identity": 0.61, "score": 103.0, "evalue": 3.17e-27, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI0022A714CF" }, "pscc": { "uniref50_id": "UniRef50_UPI0022A714CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "product": "HAD family hydrolase" }, "genes": [], "id": "IGIGPGHMMC_1313", "locus": "GCA000148625_01303" }, { "type": "cds", "sequence": "contig_1", "start": 743078, "stop": 743515, "strand": "+", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5", "UniRef:UniRef90_C4XDZ5" ], "nt": "ATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCACAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAA", "aa": "MHGIKRSKNFNFNNHKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND", "aa_hexdigest": "ceccd4252a15f9e9331a4fd27c9d8dcc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 742778, "stop": 743515, "observations": { "mutation": [ 742922, 743048 ], "stop": [ 742922, 743048 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ5", "query_cov": 1.0, "subject_cov": 0.48494983277591974, "identity": 1.0, "score": 285.0, "evalue": 3.93e-95, "gene_start": 1, "gene_end": 145, "reference_start": 155, "reference_end": 299, "reference_sequence": "MGSFLFANNIHYFLLYNTKTMDKNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLVWQRTFKKDTANKIQKFAEENKILYVIFDEEGETIYVHNAFDYWINKLFLSKKMHGIKRSKNFNFNNHKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND" }, "paralog": false, "identity": 0.9918367346938776, "score": 477.0, "evalue": 4.15e-173, "description": "Internal stop codon at 742922, 743048. Nonsense mutation around 742922, 743048.", "psc": { "uniref90_id": "UniRef90_C4XDZ5", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ5", "UniRef:UniRef50_C4XDZ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5" ], "product": "COF family HAD hydrolase protein" } }, "id": "IGIGPGHMMC_1314", "locus": "GCA000148625_01304" }, { "type": "cds", "sequence": "contig_1", "start": 743508, "stop": 744056, "strand": "+", "frame": 3, "gene": null, "product": "deoxyribonuclease IV", "db_xrefs": [ "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_C4XDZ6" ], "nt": "ATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGTTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTAGGCGCTCCTCAGAATGCTAAAAGAGTTGATCCAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAATTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGA", "aa": "MIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDPKVYKYEEYKANYSNLIKPEDIVIHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHV", "aa_hexdigest": "fa525cab8b6ad28b334c552982f3923b", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 743454, "stop": 744347, "observations": { "mutation": [ 744054 ], "stop": [ 744054 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ6", "query_cov": 1.0, "subject_cov": 0.6127946127946128, "identity": 0.9890000000000001, "score": 361.0, "evalue": 1.81e-124, "gene_start": 1, "gene_end": 182, "reference_start": 19, "reference_end": 200, "reference_sequence": "MVVCIVILFQVRARKEAIMIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDTKVYKYEEYKANYSNLIKPEDIVVHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHVWDAGYNIKNYQEFKSEINKYNLLKHIKVIHLNDSKNDLNSHKDRHENIDKGFIGIDTLAKFVHDKDFDNVPIILETPIPENGPIYDQEIAALLAYKK" }, "paralog": false, "identity": 0.9865319865319865, "score": 587.0, "evalue": 3.24e-216, "description": "Internal stop codon at 744054. Nonsense mutation around 744054.", "psc": { "uniref90_id": "UniRef90_C4XDZ6", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ6", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "IGIGPGHMMC_1315", "locus": "GCA000148625_01305" }, { "type": "cds", "sequence": "contig_1", "start": 744895, "stop": 745215, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGA", "aa": "MYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVR", "aa_hexdigest": "3cf7fa56839314ecec6b0798db4808aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12384.719499999985, "isoelectric_point": 9.474999046325685 }, "id": "IGIGPGHMMC_1316", "locus": "GCA000148625_01306" }, { "type": "cds", "sequence": "contig_1", "start": 745672, "stop": 746952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7", "UniRef:UniRef90_C4XDZ7" ], "nt": "ATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGA", "aa": "MVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDND", "aa_hexdigest": "b08ef718b132cee0c4260278337a4d56", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 745372, "stop": 746979, "observations": { "mutation": [ 745381, 745426, 745588, 745600, 746950 ], "stop": [ 745381, 745426, 745588, 745600, 746950 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ7", "query_cov": 1.0, "subject_cov": 0.6016949152542372, "identity": 1.0, "score": 684.0, "evalue": 1.41e-241, "gene_start": 1, "gene_end": 426, "reference_start": 274, "reference_end": 699, "reference_sequence": "MIINTKLDINIYIKMYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVRWFGSIFKNHYFAWFKSYTRVDYWILRKRIISFVWLTLLFVVLIYHVVLVSFRVENIWYYSTADVKNIYLKGWYYTFTNQADLLDGKSVDYPVAYLNIGFLLDTLFNLFYVVTFSPYLAIISAFLIWTLAWLFLVTLNPIKYFKNINEHKKTIVMVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDNDWVSPILSDN" }, "paralog": false, "identity": 0.9906542056074766, "score": 932.0, "evalue": 0.0, "description": "Internal stop codon at 745381, 745426, 745588, 745600, 746950. Nonsense mutation around 745381, 745426, 745588, 745600, 746950.", "psc": { "uniref90_id": "UniRef90_C4XDZ7", "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ7", "UniRef:UniRef50_C4XDZ7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 49151.044900000095, "isoelectric_point": 4.635867881774902 }, "id": "IGIGPGHMMC_1317", "locus": "GCA000148625_01307" }, { "type": "cds", "sequence": "contig_1", "start": 747099, "stop": 747212, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGA", "aa": "MKPIVESVEEEYKEYNEIIFLEVDAEKAQLFREINNR", "aa_hexdigest": "d035d9e1e33c59dcdacd73cadc0fb5e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4517.0309, "isoelectric_point": 4.431986427307129 }, "id": "IGIGPGHMMC_1318", "locus": "GCA000148625_01308" }, { "type": "cds", "sequence": "contig_1", "start": 747456, "stop": 747839, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGA", "aa": "MHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKF", "aa_hexdigest": "fee39558eb5b77120edeffab94938226", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15099.314499999984, "isoelectric_point": 9.176897239685058 }, "id": "IGIGPGHMMC_1319", "locus": "GCA000148625_01309" }, { "type": "cds", "sequence": "contig_1", "start": 748074, "stop": 749057, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_O84898", "UniRef:UniRef90_C4XDZ9" ], "nt": "ATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTTAAAACATATGTTAATATCTTTAACGCTCCTTTAGAAGAATTAGAAAAAGTTGTTTCTAAAAAAATAGCTTTAGAAATCAAAAAACTTTATGAAAGTAAGAAATAA", "aa": "MTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTFKTYVNIFNAPLEELEKVVSKKIALEIKKLYESKK", "aa_hexdigest": "ccd5083e682d8cb61b3a97a0c0d70a26", "start_type": "ATG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "5-prime", "pseudogene": { "start": 747774, "stop": 749057, "observations": { "mutation": [ 747837, 747972 ], "stop": [ 747837, 747972 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ9", "query_cov": 1.0, "subject_cov": 0.5696864111498258, "identity": 1.0, "score": 624.0, "evalue": 1.31e-221, "gene_start": 1, "gene_end": 327, "reference_start": 248, "reference_end": 574, "reference_sequence": "MTKEEIITSLKNVSTAPGIYLWKDKNDQVIYVGKAKNLRKRMHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKFWQEKFNKIKSILSFKDNSYINEIKEKMFKEAETEHFELARDLKLSWEYLKKLNEEQIIELKDIHNLDVFAYKTVDNIIYMTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTFKTYVNIFNAPLEELEKVVSKKIALEIKKLYESKK" }, "paralog": false, "identity": 0.9929742388758782, "score": 825.0, "evalue": 4.59e-303, "description": "Internal stop codon at 747837, 747972. Nonsense mutation around 747837, 747972.", "psc": { "uniref90_id": "UniRef90_C4XDZ9", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_O84898", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ9", "UniRef:UniRef50_O84898", "KEGG:K03703", "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_O84898", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O84898" ], "product": "UvrABC system protein C" } }, "id": "IGIGPGHMMC_1320", "locus": "GCA000148625_01310" }, { "type": "cds", "sequence": "contig_1", "start": 749453, "stop": 749878, "strand": "+", "frame": 2, "gene": null, "product": "6-phospho-beta-glucosidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE00", "UniRef:UniRef90_C4XE00" ], "nt": "TTGTTTAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAGTAATAAAAAAGTTGTTAAATACTTTTTAAAGTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTCTGCTAAAGTCAAAGAAATGATTAAAAATAATTACAATCATATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGCGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGA", "aa": "MFKELKRRKIKTIVTLCHYEMPLHLALKYNGFSNKKVVKYFLKYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYASAKVKEMIKNNYNHIQVGSMVLAMPFYTKSAKVEDQILKMEKE", "aa_hexdigest": "1bfbc46f82e93a63c6cc2ff99e14ed43", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 749153, "stop": 750037, "observations": { "mutation": [ 749159, 749384, 749876 ], "stop": [ 749159, 749384, 749876 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE00", "query_cov": 1.0, "subject_cov": 0.47, "identity": 0.993, "score": 269.0, "evalue": 8.27e-89, "gene_start": 1, "gene_end": 141, "reference_start": 107, "reference_end": 247, "reference_sequence": "MSFSKKFLWGTAFAANQIEGAYNEENKGLNVQDVLPKGALGHIDLNLKEFNIKRKAIDFYHKYQEDLDLLSEMGIKVLRISIAWSRIFPKGDESKPNQKGLEFYLKLFKELKRRKIKTIVTLCHYEMPLHLALKYNGFSNKKVVKYFLKYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYASAKVKEMIKNNYNHIQVGSMVLAMPFYTKSAKVEDQILKMEKEWQNLYFTDVLARGVYPKYLDNYFLKNNIKINKTKEELEVIKNNIVDYIGFSYC" }, "paralog": false, "identity": 0.9897959183673469, "score": 568.0, "evalue": 2.98e-209, "description": "Internal stop codon at 749159, 749384, 749876. Nonsense mutation around 749159, 749384, 749876.", "psc": { "uniref90_id": "UniRef90_C4XE00", "product": "6-phospho-beta-glucosidase", "uniref50_id": "UniRef50_C4XE00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE00", "UniRef:UniRef50_C4XE00" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE00" ], "product": "6-phospho-beta-glucosidase" } }, "id": "IGIGPGHMMC_1321", "locus": "GCA000148625_01311" }, { "type": "cds", "sequence": "contig_1", "start": 750139, "stop": 750240, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1", "UniRef:UniRef90_A0AB32XCF1" ], "nt": "ATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGA", "aa": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSE", "aa_hexdigest": "bf6438b1ab969c940bd1670c9fe5b05a", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 750139, "stop": 750306, "observations": { "mutation": [ 750238 ], "stop": [ 750238 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCF1", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 70.9, "evalue": 1.2e-15, "gene_start": 1, "gene_end": 33, "reference_start": 1, "reference_end": 33, "reference_sequence": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSEWISNNYFRNKVHIIFKSEGGKF" }, "paralog": false, "identity": 0.9818181818181818, "score": 115.0, "evalue": 1.05e-35, "description": "Internal stop codon at 750238. Nonsense mutation around 750238.", "psc": { "uniref90_id": "UniRef90_A0AB32XCF1", "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCF1", "UniRef:UniRef50_A0AB32XCF1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3992.4436000000005, "isoelectric_point": 4.676394081115721 }, "id": "IGIGPGHMMC_1322", "locus": "GCA000148625_01312" }, { "type": "cds", "sequence": "contig_1", "start": 750376, "stop": 750528, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTGTTGTTAACTTTATTTTAGTTTGCTTAATATATTTAATTGGATTGTTTCTATTTCCTCTCACTTATTTCAAATGTAAAAACTATGAAACAGATTTAAAAAACAATGTTAAGTTCAATGTAGACTTATATGGAGAAGCTAAATAA", "aa": "MKLVVNFILVCLIYLIGLFLFPLTYFKCKNYETDLKNNVKFNVDLYGEAK", "aa_hexdigest": "70377fa20cdf25e2d7eb34176df54173", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5910.058200000002, "isoelectric_point": 8.638263893127444 }, "id": "IGIGPGHMMC_1323", "locus": "GCA000148625_01313" }, { "type": "cds", "sequence": "contig_1", "start": 750620, "stop": 750886, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGA", "aa": "MITLSSLKVALKIKLLSTKAKIKGIIIIAAYITIWVVLTNVFALVVVSRFRKLSHDANAPIPIVAILAAKAIQDNFFILSIIDNCSSI", "aa_hexdigest": "7af78ef7a1ecb8640d0d73e7f2e97d05", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.599199999993, "isoelectric_point": 10.106337928771971 }, "id": "IGIGPGHMMC_1324", "locus": "GCA000148625_01314" }, { "type": "cds", "sequence": "contig_1", "start": 751256, "stop": 751747, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "ATGCCTGAAGTAGTTTTAGTAGATAAGGAAAACGAAAAGAAACAAATATTAAAAGATCTTTTAACAAATGTTTATGTTAATGACATTGCTGAGAGACATAAACTTAATAGTACTCAAGATGTTTTAAACATAGTTGAATATCTTGCTTCAATAGTTTCATCTCAATGTAATTCAAGTAGAATTGCCAACTCTTTTAATTCTTTAAATAATAAAAAGACTTCTACTTATCAAATAGATAAAATATTAGATGCACTAGAAGATTCTTTCTTAATAGATAAAATAAGAAAATATGATATTAAAGGTTTAAAACTTCTTTCGGGTCAATATAAATACTATTTTGTTGACAACGGATTAAGAAATGTAGCTCTCAATTTTAGAAATCTAGGACAAGGTCAATTAATCGAAAATGCCTTTTTTAATGCTCTAAAAATAAATTACTTCGATATTAGTTATGGGGCAATTAATCAAAAAAATAATATTAATAATCAATGA", "aa": "MPEVVLVDKENEKKQILKDLLTNVYVNDIAERHKLNSTQDVLNIVEYLASIVSSQCNSSRIANSFNSLNNKKTSTYQIDKILDALEDSFLIDKIRKYDIKGLKLLSGQYKYYFVDNGLRNVALNFRNLGQGQLIENAFFNALKINYFDISYGAINQKNNINNQ", "aa_hexdigest": "39cf088e65a2a8517a98ccc4d42a0712", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 127, "aa_cov": 0.7361963190184049, "hmm_cov": 0.952755905511811, "evalue": 5.618245567049488e-14, "score": 48.20418930053711, "start": 27, "stop": 146 } ], "seq_stats": { "molecular_weight": 18721.01909999999, "isoelectric_point": 8.526024436950681 }, "id": "IGIGPGHMMC_1325", "locus": "GCA000148625_01315" }, { "type": "cds", "sequence": "contig_1", "start": 751772, "stop": 751954, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTAAATTCATTTCAAGATCAAGATAATATTGATATTTATGTTACTGGTAGTAATTCTAAATTCTTATCAAAAGATATAGCTACTACTTTTGCGGGCCGAGGTATGGAGATTAATGTTTTGCCTTTATCATTTCAAGAATATTTAAACTTTAGTAATCTTGATATAAATGAAGCTTGA", "aa": "MVLNSFQDQDNIDIYVTGSNSKFLSKDIATTFAGRGMEINVLPLSFQEYLNFSNLDINEA", "aa_hexdigest": "e17668e6b138d8966b636308086a5ccf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6750.442699999998, "isoelectric_point": 4.0500284194946286 }, "id": "IGIGPGHMMC_1326", "locus": "GCA000148625_01316" }, { "type": "cds", "sequence": "contig_1", "start": 752054, "stop": 752263, "strand": "-", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "nt": "ATGAATAAAGTTATTAAAAGAGACAATTATTTAAATATGCTAATTGATAAAATCAATAACAAAAAAATTAAAATAATTACAGGTATAAGAAGAGTAGGTAAAAGTTTTTTACTCTTCAATTTATTCTATAGTTATTTATTAGCTAATAAAGTTGAAGCTGACCAAATAATAACAATAAAACTTGACCGAGTAGAAAATGAAGAACTTTGA", "aa": "MNKVIKRDNYLNMLIDKINNKKIKIITGIRRVGKSFLLFNLFYSYLLANKVEADQIITIKLDRVENEEL", "aa_hexdigest": "f8e9f5ff272b7e96a6d24a4f41a9aef3", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFEF55", "query_cov": 0.9420289855072463, "subject_cov": 0.9154929577464789, "identity": 0.615, "score": 81.3, "evalue": 6.32e-19, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A4Y8VNC6" }, "pscc": { "uniref50_id": "UniRef50_A0A4Y8VNC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1327", "locus": "GCA000148625_01317" }, { "type": "cds", "sequence": "contig_1", "start": 752608, "stop": 752775, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_UPI000FF82D94" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTTCAAATAGAAGAAGTTAGAAACAATTATCAAAACTTTTTATGTTTGTTTAGCGAAGCGAGACTAAGAGTTAGATTGTGTAGAAAAGAAAACATGCATTTTGGTGATGAATTACAAATAGACGCTTGTATCTATGAATGA", "aa": "MIKIAELLKVQIEEVRNNYQNFLCLFSEARLRVRLCRKENMHFGDELQIDACIYE", "aa_hexdigest": "8442bdff9ae7f8996aac78b540fef29a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 1.0, "score": 115.0, "evalue": 8.11e-33, "valid": true, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6647.705799999998, "isoelectric_point": 5.773216056823729 }, "id": "IGIGPGHMMC_1328", "locus": "GCA000148625_01318" }, { "type": "cds", "sequence": "contig_1", "start": 753373, "stop": 753633, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTATTGATTATTTTTTAGATTGCTTAATTATTTCCTATAATAAATTAATAGATGTAAATAAAAAAGATTATTTAGTAAATCAAATAATCAAAATTTTAACTACTAGCTTAATTCCTTTATCAACTAAAGAAATAAGTGAACAACTTGTTTCAATTTCAAGAAGAACACTTGAAAGAAAACTTAAAGTCTTATTAGAACAAAATAAAATCAAAAAAGTTGGTCAATATAAAAATGCTAAATATAAATTAACATAA", "aa": "MPFIDYFLDCLIISYNKLIDVNKKDYLVNQIIKILTTSLIPLSTKEISEQLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT", "aa_hexdigest": "79b17fedf6394333dd0206d02b56d92c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10118.980499999989, "isoelectric_point": 9.714950370788571 }, "id": "IGIGPGHMMC_1329", "locus": "GCA000148625_01319" }, { "type": "cds", "sequence": "contig_1", "start": 753655, "stop": 754419, "strand": "-", "frame": 2, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05", "UniRef:UniRef90_C4XE05" ], "nt": "ATGGTTAAAAAAGAAAAAAGGTTTTTTGATTATTCATTTTTAAAGAACATTATTAATAATGAAATTAACAAAAAATTAAAACAAATTTATGAGTTAAATGGACTTATAAATGATTTTTCTAATATTCCAGAACAAACTAAAAATACTTTAATTAAAAATAGTATTTTTCAAAGTGTTACTTCTTCAAACAAAATTGAAAATATTGTTTCAACTACAAACAAAATTGAAAATATTATTTTTAATAATAAAAAATATACAGGCGAAAACGAATTAGAAATTCAAGGTTATAATGATGTATTAAATATTATTTTAAATAATTTTGATAATATAGATATTACTAAATCAAACATCTTAAATTTACATAATCAATTATATAAATATAGTAATAAAACTTATTTAAAAGGAAAGTTTAAAGAAATGAACAATTATATTTTTTCAATTGACCAAAACAACTATAAAACTGTTATATTTGAACCGGTTGATCATTCTTTGGTTGATGATTACATAACAAAAATATGTGATGAATACAATAAAAGAAATGATGAAAAAATAATTGATCCTTTAATACTAATTCCAACATTTATTTTGGATTTTTTATGTATTCACCCTTTTAGTGATGGCAATGGTAGAATTTCAAGATTATTAACTAACTGTTTATTATTTCAAAATGATTTTATGATTAATAAATACATTAGCTTTGAAAAAATCATAGAAGAAAAATTAAATCAATATTATTTTGCTTTAAGAAAAAGCTCAACTAATTGA", "aa": "MVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEEKLNQYYFALRKSSTN", "aa_hexdigest": "6cd3dab39bcd3ac8577550431ad2fa0d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 753373, "stop": 754428, "observations": { "mutation": [ 753657 ], "stop": [ 753657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE05", "query_cov": 1.0, "subject_cov": 0.7236467236467237, "identity": 0.992, "score": 470.0, "evalue": 1.86e-165, "gene_start": 1, "gene_end": 254, "reference_start": 4, "reference_end": 257, "reference_sequence": "MIIMVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEENLNQYYFTLRKSSTNWQYSQNDYLPFIDYFLDCLIISYNKLIDVNKKDYLENQIIEILTTSLIPLSTKEISERLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT" }, "paralog": false, "identity": 0.9715099715099715, "score": 652.0, "evalue": 7.32e-240, "description": "Internal stop codon at 753657. Nonsense mutation around 753657.", "psc": { "uniref90_id": "UniRef90_C4XE05", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE05", "UniRef:UniRef50_C4XE05" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05" ], "product": "Fido domain-containing protein" } }, "id": "IGIGPGHMMC_1330", "locus": "GCA000148625_01320" }, { "type": "cds", "sequence": "contig_1", "start": 754820, "stop": 755677, "strand": "-", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50", "UniRef:UniRef90_UPI000FEE809E" ], "nt": "ATGCTTAAAAGCGCAGGTTTAATTCTAAAAGATCCTGAAACCAATAAAGAAGGAATAACTATAGCAGCTATTTTGCTTTTTGGAAAAGATCAAACTATTATGTCTGTTCTTCCTCAATATCGTACAGATGCTATTTTTAGAGTTAAAAATAAAGATAGATATGATGATAGAGAAATAGTAGCAACCAATTTAATCGATAGCTATGAGAAATTAATTAATTTTGGTGAAAAACACTTAAGTGATATTTTTGTTTTAGATGGTATTGTAAGTGTTAGTGCTCGTAATTTGATACTTAGAGAAATCATTTCTAATACACTAGCTCATAGAAACTATGCAAGCAATATGACAGCTACTATTGTCATTGATGAAAACAATATAACTGTTGAAAATGATAATAGTTCAAGCAGTAAAGAGGAATTAGATCCTAAAACCTTTAGACCAAATCCAAAAAATCCACCAATTTCAAAAGTTTTTAGAGAAATTGGTTTAGCTGATGAATTAGGTTCAGGAATTAGAAAAACTTATAAATATACAAAACTTTATTCAAATAATAAACCTACATTTTTGGATAGTAACATATTTAAAACGATTATTCCTATTCCAAAAATTGCTACATTAAAAGTTGGTAAAATGACCTCTAATATAGATACAGATAATAAAAATATTACAAACAAAAAACAATTAAATATTGAAAGAATTTTCAATTTAGTTAAAGAAAATTCTACAATAAAAAGAAGTGAAATTGCAGCAATTTTAAATTTAACTGAAAAAACTATTTCAAGATATATTAGAGAAATAAAAAACATAAAATATGTTGGAAAAGGTAAAAATGGTTATTGGGAAATTATTGATAAATAG", "aa": "MLKSAGLILKDPETNKEGITIAAILLFGKDQTIMSVLPQYRTDAIFRVKNKDRYDDREIVATNLIDSYEKLINFGEKHLSDIFVLDGIVSVSARNLILREIISNTLAHRNYASNMTATIVIDENNITVENDNSSSSKEELDPKTFRPNPKNPPISKVFREIGLADELGSGIRKTYKYTKLYSNNKPTFLDSNIFKTIIPIPKIATLKVGKMTSNIDTDNKNITNKKQLNIERIFNLVKENSTIKRSEIAAILNLTEKTISRYIREIKNIKYVGKGKNGYWEIIDK", "aa_hexdigest": "b5b1503d7d9e328809bf2bdedf2ec402", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEE809E", "query_cov": 1.0, "subject_cov": 0.8431952662721893, "identity": 1.0, "score": 543.0, "evalue": 3.31e-194, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_R9RA50" }, "pscc": { "uniref50_id": "UniRef50_R9RA50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50" ], "product": "Helix-turn-helix type 11 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1331", "locus": "GCA000148625_01321" }, { "type": "cds", "sequence": "contig_1", "start": 756097, "stop": 756240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGAAGAATTGAAAAAACTTTTAATCCAAGGTGAAGGCGAAGAAATAGAATTCAAAACATGCAAAGATGCCTTATCTAAAAGTGTTTTTGATACCGTTTCAGCTTTTAGCAATAAAAATGGAGGTCATCTAATTTAG", "aa": "MNKEELKKLLIQGEGEEIEFKTCKDALSKSVFDTVSAFSNKNGGHLI", "aa_hexdigest": "402a1505f0efd2c1f5f993fef1c1b973", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5227.917600000001, "isoelectric_point": 5.684433555603028 }, "id": "IGIGPGHMMC_1332", "locus": "GCA000148625_01322" }, { "type": "cds", "sequence": "contig_1", "start": 756816, "stop": 756998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527068.1", "SO:0001217", "UniParc:UPI0001A8C360", "UniRef:UniRef100_C4XFH5", "UniRef:UniRef50_C4XFH5", "UniRef:UniRef90_C4XFH5" ], "nt": "ATGTTATTAGTTATTTTAAAAGAAAACATCAATGACAAACAAAAATATTTAAAAGAATATTTAATAACATTCTTATTAAGTGATATATTAGAACACTATAATGTCAAAAATGACTATAAAATAGAGGAATTATTAAAATTCTTAGCTTCTAGTTCTTCATCTTTAACTAATACTAAAAAATAA", "aa": "MLLVILKENINDKQKYLKEYLITFLLSDILEHYNVKNDYKIEELLKFLASSSSSLTNTKK", "aa_hexdigest": "af8ef68bb503186a0d73662924daf43a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C360", "ncbi_nrp_id": "WP_013527068.1", "uniref100_id": "UniRef100_C4XFH5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C360", "RefSeq:WP_013527068.1", "UniRef:UniRef100_C4XFH5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFH5", "uniref90_id": "UniRef90_C4XFH5" }, "psc": { "uniref90_id": "UniRef90_C4XFH5", "uniref50_id": "UniRef50_C4XFH5" }, "pscc": { "uniref50_id": "UniRef50_C4XFH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7095.2107, "isoelectric_point": 7.947291755676269 }, "id": "IGIGPGHMMC_1333", "locus": "GCA000148625_01323" }, { "type": "cds", "sequence": "contig_1", "start": 757682, "stop": 757882, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTTAATGCACAAAATATATATTTAAGTGCTTTAAAAGCAAGCGCTAAAGATAATAGTTATAATGATTTGTTAAATAATCTTAATAAAAAAGACTTAACTTTATTAAGATATTGCATAGAAAATAAAAAAAGTATTATTCTTATTAAAGATTTAGCTTTAGTCTTTGGCGTTACTTCTAGAGCTATATCAAAATGA", "aa": "MALNAQNIYLSALKASAKDNSYNDLLNNLNKKDLTLLRYCIENKKSIILIKDLALVFGVTSRAISK", "aa_hexdigest": "876e075ed2bdb5a0ab0810fd8030f711", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7371.577999999999, "isoelectric_point": 9.611349678039552 }, "id": "IGIGPGHMMC_1334", "locus": "GCA000148625_01324" }, { "type": "cds", "sequence": "contig_1", "start": 757904, "stop": 758050, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGATGATTATGATTTCAAAGGATTTAATTCTTTTGAAGAATATTATAGTAATAACTTAAATGAATATTATCAAGCATTACAAATGGATTTAAGTCCTTTATTTTATAATGGACGTAATAAACCGCCACATTTAGAAAAATGA", "aa": "MLDDYDFKGFNSFEEYYSNNLNEYYQALQMDLSPLFYNGRNKPPHLEK", "aa_hexdigest": "04e29d9c5ea222cc438588b1adb4d885", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5860.366800000001, "isoelectric_point": 4.499567985534668 }, "id": "IGIGPGHMMC_1335", "locus": "GCA000148625_01325" }, { "type": "cds", "sequence": "contig_1", "start": 758417, "stop": 758686, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGGAATTAGATCTATCATTTAAATATACAGATGAGCTTGTCAATTATCTTCTAGAAATTGAAAAATATAAAAGTTCATTAGAATTTTTGTCTTTACCTACAAGAAGTAAACAAAGAATTCAATTTGAAGCAAAAATCAAACAAACTCATTTTTCTACAAGCATTGAAGGCAATGTTCTAAACATTGAACAAGTTAAAAACGTTATAAACAATAAAACACATCAAACTAGAATTAAAGCTGAACAAGAAGTATTAAATTATTGA", "aa": "MKKELDLSFKYTDELVNYLLEIEKYKSSLEFLSLPTRSKQRIQFEAKIKQTHFSTSIEGNVLNIEQVKNVINNKTHQTRIKAEQEVLNY", "aa_hexdigest": "6230732531598f25a9e3c8983eecf3a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10563.959499999992, "isoelectric_point": 8.860550880432129 }, "id": "IGIGPGHMMC_1336", "locus": "GCA000148625_01326" }, { "type": "cds", "sequence": "contig_1", "start": 759488, "stop": 759817, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527071.1", "SO:0001217", "UniParc:UPI0001F59884", "UniRef:UniRef100_A0AB32XCI0", "UniRef:UniRef50_A0AB32XCI0", "UniRef:UniRef90_A0AB32XCI0" ], "nt": "GTGCTTATTGATAAGGATGGTAGTATTTATATCGACATTTTAGGAATTGAAAAATTTCCTACTAAAACATTGACTAGAGAAGAACTTAACGAATATCAACTTTTTGTACACAATTTAGGTGTGACAGAAAGCACAATATCAAAACTTCTTTATACAAAAATCAAATCAACAAATAGTTTTTATAACTTAATAGAATCTAAAATTGAAAAATTAAAAAATCTTTTACTCACTCTAAATTTAGACAAAAATAAATTAGAAAGCGAAATAAAAGATTTTACTCATAACATCTGTAAAATCAATAAAAAAATAGGTGAATTAATTCAATTCTAA", "aa": "MLIDKDGSIYIDILGIEKFPTKTLTREELNEYQLFVHNLGVTESTISKLLYTKIKSTNSFYNLIESKIEKLKNLLLTLNLDKNKLESEIKDFTHNICKINKKIGELIQF", "aa_hexdigest": "4baa41c4450eac7e5c49f59b799df526", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59884", "ncbi_nrp_id": "WP_013527071.1", "uniref100_id": "UniRef100_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59884", "RefSeq:WP_013527071.1", "UniRef:UniRef100_A0AB32XCI0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCI0", "uniref90_id": "UniRef90_A0AB32XCI0" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCI0", "uniref50_id": "UniRef50_A0AB32XCI0" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCI0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.667799999981, "isoelectric_point": 7.791342735290527 }, "id": "IGIGPGHMMC_1337", "locus": "GCA000148625_01327" }, { "type": "cds", "sequence": "contig_1", "start": 760163, "stop": 761362, "strand": "-", "frame": 1, "gene": null, "product": "RNA (S1 domain)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "nt": "ATGGCAATAGACCCTGCTTTTGTTAATGGTTGCAAAATTGCAATCTTAGATCCTCAAGGTAAGTTCTTAAAAAAAGCTTTAATTTATCCTAACCCTCCTCAAAATTATGTTGATAAAGCTAAAGAAATAGTTAATAAACTATTAGATTCATACAAAGTTAATATTATTGTTATTGGCAATGGAACAGCTTCAAGAGAAACTGAAGAATTTATTGCTAATTTATTAAAAGAAAGATCAAAAGTAAATCCTAATGAAGATATTTCTTATGCTGTTGTTAGTGAAGTAGGAGCTAGTGTTTATTCAGCTTCTAAAGTTGCTATTGATGAATTTCCAGATTTTAGTGTTGAAGAAAGATCAGCTGTTAATATTGGACGTCGTTTCCAAGATCCTCTTAATGAATTAGTTAAAATTGATCCTAAAGCTATAGGGGTAGGTCAATATCAACATGATGTTAATCAAAAAGATTTATCTAAGGCTTTAGACTTTAAAGTTGATAAAGTAGTTAACATGGTTGGAGTTGACTTAAATAGTGCTACTAAAGAAATCTTATGCTACATTTCAGGTTTAACTAAAACATTAGCTCAAAACATTATTGATTATCGTGAAAAAATCAATAAATTTAAAGAAAGAAAAGAACTATTAAAGGTTAAAGGTTTAGGCGAAAAAGCCTATGAACAAGCGGTTGGCTTCTTAAGAATCCATGATTCTAAAAACTTTTATGACCGCACAGCTATTCACCCTGAATCATATCCTTTAGCAATTAATATTGTTAATGCGCTTCAATTAGATCTAAATGATATAGACTTAAAAACTATTGAACAACAAGATGTTAATAAATTAGCTAAAGACTTTAATTCAAATGAATATGATGTTAAGTTAATATTAGATTCTTTATCTAATCCAACTAAAGATATTCGTGATGACAAAGATGGCTACATTCTTAAAAAAGATGTTCTTAAGTTAGAAGACTTAAAACCAGGTATGATTCTAGATGGTTCAGTGCAAAATATTACAGACTTTGGTATCTTTGTTTATATTGGAATTAAGCAAGCTGCTTTAATTCACATTTCAAATATGAAAAGAAATAAAGATCACTACATCAAACACTCTAGCGAAGTAGTTAAAACAGGAGATAACATTAAAATCCAAATCCTTGAAATAGATAAAGAAAGAGAAAGAATTCAAGGCAAATTAATTTGA", "aa": "MAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDVNKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLI", "aa_hexdigest": "75e1cef2a4fad007d42eb641bf714e28", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PA84", "query_cov": 1.0, "subject_cov": 0.9322429906542056, "identity": 0.539, "score": 411.0, "evalue": 8.69e-139, "valid": false, "product": "RNA (S1 domain)-binding protein", "uniref50_id": "UniRef50_A0A3B0PA84", "kegg_orthology_id": "K06959" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PA84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "product": "RNA (S1 domain)-binding protein" }, "genes": [], "id": "IGIGPGHMMC_1338", "locus": "GCA000148625_01328" }, { "type": "cds", "sequence": "contig_1", "start": 761387, "stop": 762310, "strand": "-", "frame": 1, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "KEGG:K06959", "SO:0001217", "UniRef:UniRef50_L0RUW1", "UniRef:UniRef90_C4XFG9" ], "nt": "ATGGATTTATCTAAAAAATTTGTAGCACAAAAATTAAACTTAAAAGAAGACCAAGTTGAAGTTGTTCTTAAGTTAATGGATGAAGGAGCAACAGTACCTTTTATTGCCCGTTATCGTAAAAATGTAACTGGTGGACTTGATGAAGAAGTTATTCAACAAATAAGTGACATGTATGTTTACAATGTTGAATTAAACAAAAGAAAAGAAGCTATTATTGACATCTTAAAAGAAAAAGGATTACTCACACCTGAAATTGAAAATAAATTAAAAGCAGCTGAAACTAAAGCTGAAGTTGAAAACATTTATGAACCATTTAAAGTTGGTAAAAAAACTAAAGCTAGTGAAGCTATTGCTTTAGGTTTAGAACCTTTAGCTAAAGCAATTATGGAAGCAACTGATGAAAGTTTTGATCCTTATAAAGAAGCTAAAAAATACTTAAATGACAAAGTACCAACAGTTGAATTTGCTATTGAGCAAGCTCAATACATTATTAGCCAAATTATGTCTCAAGATGTTGAAAATAGAGAATATGTTAAAAAACAAATTTATGATTTTGGTTTCATTGTAACAAAAGTTAAAAAGAATGCTGAAGATGAAAAAGAAGTATTTCAACAATACTATGATTATAAAGAAAGAATCAAGTTTATTCCTAACCATCGTGTTTTAGCAATTAGTCGTGGTGAAGACAAAAAGATTTTAAGTTATGACATGACTTATAATTTAGCTAAAATTACTTATGACTTAAACAACAAATATTTTAAAATCAAAAGAACAGGCAAGATTTTGAACGAATCATTAAAAGACAGTTTAGATCGTTTGATTTTACCTTCAATTATTAGAGAAATCAAAAGTGATCTTTTTGCTCGTGCAGAAAAAGAAGCAATTAACCTTTTCGCAACTAATCTTGAAACAATGTTACTATGA", "aa": "MDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLL", "aa_hexdigest": "f3778c4d57722586d524453307c6b7d7", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 761084, "stop": 762322, "observations": { "mutation": [ 761389 ], "stop": [ 761389 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG9", "query_cov": 1.0, "subject_cov": 0.425207756232687, "identity": 1.0, "score": 578.0, "evalue": 7e-202, "gene_start": 1, "gene_end": 307, "reference_start": 5, "reference_end": 311, "reference_sequence": "MEENMDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLLWPAVKNKTVMAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDANKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLIWED" }, "paralog": false, "identity": 0.9951456310679612, "score": 795.0, "evalue": 2.25e-289, "description": "Internal stop codon at 761389. Nonsense mutation around 761389.", "psc": { "uniref90_id": "UniRef90_C4XFG9", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_L0RUW1", "kegg_orthology_id": "K06959", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG9", "UniRef:UniRef50_L0RUW1", "KEGG:K06959" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUW1" ], "product": "S1 motif domain-containing protein" } }, "id": "IGIGPGHMMC_1339", "locus": "GCA000148625_01329" }, { "type": "cds", "sequence": "contig_1", "start": 762615, "stop": 763448, "strand": "+", "frame": 3, "gene": null, "product": "CTP synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "nt": "GTGACTAAATTTATTGTGACAACTGGTGGAGTTTTATCTGGTTTAGGTAAGGGCGTTACCAGTGCAAGTATTGGAAATTTATTAAAGGCACAAGGCTTTAAAGTCTTTGCTTTAAAACTTGATCCATATTTAAATATTGACCCAGGAGTAATGAGTCCCATTGAACATGGTGAAGTATTTGTAACTGCTGATGGTGGTGAGACTGACTTAGACTTAGGACACTATGAAAGATTTATTGATGTTAAGCTAAGCAAGGAAAGTAACTACACAAGTGGAAGAATTTTTACTCGCATTTTTGAAAAAGAACGTCAAGGACTTTATGGTGGAAAAACTGTTCAAATAGTACCTCATGTTATTGATGAAATTATTAGCATTATCGAAAATACTGCTAAAAAACAAAAACCAGATTTCATGTTAATTGAAATCGGAGGAACTGTTGGAGACATTGAAAGTAATCCTTATATTTATGCAATTAGTAAGTTTGCTAGTTTAAATCCTAAAAGTGTTTTCTTTGCTCACTTAGCATTTATTCCTTATTTAACTGCTTCAAAAGAATACAAATCAAAACCATCTCAAGTTTCAATTGCTTCTTTAAGATCATTTGGAATTAACCCTAATATTTTACTTTTAAGAAGTCAAGGCGAAGTTGATGAAAGAATTATTAATAAAGTTGCTGAAGCTTCATTTTTGGACCCTAATCATGTAATTGGAGTTCCTGATAAAAGCAGTATCTATGAAATTCCAATGTTCTTATATGAACAAAATATTTTAAAAATAATTTACTCACATTTTGAAATTAAAAAACAAATTAATGACAAGGTTTATGATTCATGA", "aa": "MTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFEIKKQINDKVYDS", "aa_hexdigest": "5fdc543bcbfc055e2ffa345b4133e7d2", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387385AD", "query_cov": 0.9855595667870036, "subject_cov": 0.8694267515923567, "identity": 0.6809999999999999, "score": 387.0, "evalue": 9.22e-133, "valid": false, "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_A0A223MAG4", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "product": "CTP synthase" }, "genes": [], "id": "IGIGPGHMMC_1340", "locus": "GCA000148625_01330" }, { "type": "cds", "sequence": "contig_1", "start": 763458, "stop": 764225, "strand": "+", "frame": 3, "gene": null, "product": "CTP synthase", "db_xrefs": [ "EC:6.3.4.2", "GO:0003883", "GO:0006221", "GO:0006241", "KEGG:K01937", "SO:0001217", "UniRef:UniRef50_Q98PQ3", "UniRef:UniRef90_C4XFG8" ], "nt": "TTGAATAAATATCACGCAACTAAAAATTATAAAATTAACTTACTTTTAGTTGGTAAATATACTGAATTAGAAGATGCTTATTTATCAATTATTTCTTCATTAAAAATAGCTGCTGTTCATCAAAATGTCGAATTAAAATATGACTTAATTGATGCTGATTTAGTTAACAATAAAAACATTGAAAAATTAACAAAAGCACATGATGCAGTAATGATTTTACCAGGCTTTGGTGCTAGAGGTTTTGAATCTAAAGTTAATGTTACGACTTACACAAGAGAACACAAAATTCCTACTTTAGGTATTTGTTTAGGTTTTCAAGCAATGTCAGTAGCTCAAGCTCGATTATTAGGCATTAAAGATGCAACCAGCAAAGAATTCGCTGAAAATGGTAAAAAACAAACTTTTGTTTTAACTCCTTTTTATGAAAATGGAGACAAAATGGCTATTGGTGGCACACTAAGACTTGGTGAAGATCAAGTTCAAGCTAAAAATAACACCTTAGCTCAAAAAATTTATGGTTCAAATATTTTCTATGAACGTCACAGACATAGATATGAAATTAATCCTGAATACTTAGAAAAATTACAAGATGATGAATTTACATTCAGTGCAATTCATCCAACTAAAAAAGTGGCTGAAATTTGTGAAGTTAAAAACCATCCTTTCTATTTAGGTGTTCAATATCACCCAGAATTTAGTACTAGAGTTTTAGCTTCAAATCCACTTTTTGATAATTTCTTCCAAGCAATTATTAAAAATCAAAAATAG", "aa": "MNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVTTYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK", "aa_hexdigest": "f2788603ab52f953be033279497f7e9f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 763158, "stop": 764225, "observations": { "mutation": [ 763446 ], "stop": [ 763446 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG8", "query_cov": 1.0, "subject_cov": 0.46703296703296704, "identity": 0.992, "score": 510.0, "evalue": 1.8e-178, "gene_start": 1, "gene_end": 255, "reference_start": 292, "reference_end": 546, "reference_sequence": "MNPIVLRRIIVTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFGIKKQINDKVYDSWKVFLNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVATYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK" }, "paralog": false, "identity": 0.9915492957746479, "score": 699.0, "evalue": 5.43e-255, "description": "Internal stop codon at 763446. Nonsense mutation around 763446.", "psc": { "uniref90_id": "UniRef90_C4XFG8", "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_Q98PQ3", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG8", "EC:6.3.4.2", "UniRef:UniRef50_Q98PQ3", "KEGG:K01937", "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PQ3" ], "product": "CTP synthase" } }, "id": "IGIGPGHMMC_1341", "locus": "GCA000148625_01331" }, { "type": "cds", "sequence": "contig_1", "start": 764237, "stop": 764335, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATTACAAAAAAACATTAAATATGCCTCAAACTAATTTCGATATGCGTGCTAACTTAACAACTAAAGAGCCAGCATTCCGAAAAGAATGA", "aa": "MAIDYKKTLNMPQTNFDMRANLTTKEPAFRKE", "aa_hexdigest": "77547607ab02993b7910716ce7b0018c", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3804.3769999999995, "isoelectric_point": 9.397443580627442 }, "id": "IGIGPGHMMC_1342", "locus": "GCA000148625_01332" }, { "type": "cds", "sequence": "contig_1", "start": 764555, "stop": 764800, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGCTTGAGGAAGCACACTTAAAGAAAAATGAATTAAGTGTTGTTGACTTACGTAAAAAAGCTGCTCTTTATGCTCAAGATCAAGTTAATAAACAAGCTGAACAATTCAAAAGCATGCAACTTTTATGTGACTTTAAAAAATACTACATTACTAAAGATCCTAAATTCGAAGCTCAACAATTAAGACTTTTTAAAAAGATGATTTTTAGTGGTCTTATTTACAAAGGATTAAAACCTGTTTATTGA", "aa": "MLEEAHLKKNELSVVDLRKKAALYAQDQVNKQAEQFKSMQLLCDFKKYYITKDPKFEAQQLRLFKKMIFSGLIYKGLKPVY", "aa_hexdigest": "7ce3774468e2a5fef7a2aaf7e5f48aaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 74, "aa_cov": 0.9135802469135802, "hmm_cov": 1.0, "evalue": 2.0338006302983295e-11, "score": 39.24574279785156, "start": 8, "stop": 81 } ], "seq_stats": { "molecular_weight": 9619.299899999995, "isoelectric_point": 9.576665687561036 }, "id": "IGIGPGHMMC_1343", "locus": "GCA000148625_01333" }, { "type": "cds", "sequence": "contig_1", "start": 765131, "stop": 765439, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ], "nt": "ATGCAATATGTAAGCCCACTTAATAAAAATGTATGTCCTGTAGTTATAGGCCACCACGTTACTTTAGAAAGTGGTAGTGGTTTAGTTCATATTGCTCCTCTTTTTGGTGAAGATGACTTCCAAATTGGAATCAAAAACTCACTTAAAATGATTATGCATATTGAAGATGATGGAAAAATTAATAAAGAAGGTGGAGCTTACCAAGGTCAATTTTATGAAAAAGCTAATGCTTTAATTATTGAAGAATTAACTAAATCTAAAAACTTACTTTTAGAAAGCTCAATAACTCACTCATATCCACATGACTGA", "aa": "MQYVSPLNKNVCPVVIGHHVTLESGSGLVHIAPLFGEDDFQIGIKNSLKMIMHIEDDGKINKEGGAYQGQFYEKANALIIEELTKSKNLLLESSITHSYPHD", "aa_hexdigest": "18fec76ff2f8107dfec1b0fcc070f139", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9R0", "query_cov": 0.9901960784313726, "subject_cov": 0.9099099099099099, "identity": 0.545, "score": 114.0, "evalue": 8.22e-31, "valid": false, "uniref50_id": "UniRef50_A0A432F9R0" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11306.75819999999, "isoelectric_point": 5.5876367568969725 }, "id": "IGIGPGHMMC_1344", "locus": "GCA000148625_01334" }, { "type": "cds", "sequence": "contig_1", "start": 765943, "stop": 766098, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTACTCAAAGACGTAAAATATCAGCTCCATTTTTGCTAATTATTTTAAGAGGATCAACAACATTACCTTTTGATTTAGACATTTTTTCACCTTTAGCATCTAAAACAAAACCGTGTGAAACAATGTTTTTGTATGGTGCTTTACCATTATAA", "aa": "MATQRRKISAPFLLIILRGSTTLPFDLDIFSPLASKTKPCETMFLYGALPL", "aa_hexdigest": "a57b935c98712aa1a5d40de0d71e357f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5684.778299999999, "isoelectric_point": 9.622825050354006 }, "id": "IGIGPGHMMC_1345", "locus": "GCA000148625_01335" }, { "type": "cds", "sequence": "contig_1", "start": 766190, "stop": 766831, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "ATGCTCGGTAATTTAAATGGTTTTAAATATGACTCAAATCTCAAAAGAATTGGCGTACATGCCTATATTAAAGAGCAATTGGAAAAAGTTAAAGCTCAAGTTATTGAAGCTTATGATGAATATAAATTCATTAACGTTATTAAACTTTTAAATAATTATGTAGTTGAATTATCAAGCTTTTATTTAAACATTTCAAAAGATGTTTTATATGTCGAAGCTAAAGATTCAATTAACCGTCTTATGACTTTAACAAATATTTATGAAATTGTTGATTTTCTAATTATTGCTCTTGCTCCAATTCTTCCAACTACAGCTGAAGATGCTTACAAAAACTTTAATAAAGTAAATAAAGCTGAAAGTGTTCATCTCGAAACTTTAGCTAACCCACTCAAAGTTAACGAACAAGTTTTAACTCAATGGCAAGACTTTTTTAAACTTCGTGATGAAGTGAACTTATCACTTGAAAATGCAATTAAAGAAGGTTTAATTAAAAGAACTAATGAAGCTAAAGTTGTAATTAAAAGCGATAGCGACTTTATTAAAAGTCTTGATTTAAGACAATTATTAATGGTTGGTAAAGTTGAATTTGGAGCTCAAACTTCTGTTTCAGTTTTCAAATCAATTAAATGCCAAAGATGTTGA", "aa": "MLGNLNGFKYDSNLKRIGVHAYIKEQLEKVKAQVIEAYDEYKFINVIKLLNNYVVELSSFYLNISKDVLYVEAKDSINRLMTLTNIYEIVDFLIIALAPILPTTAEDAYKNFNKVNKAESVHLETLANPLKVNEQVLTQWQDFFKLRDEVNLSLENAIKEGLIKRTNEAKVVIKSDSDFIKSLDLRQLLMVGKVEFGAQTSVSVFKSIKCQRC", "aa_hexdigest": "7d34ec40ca24057487bbf102d1303a56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 154, "aa_cov": 0.7136150234741784, "hmm_cov": 0.948051948051948, "evalue": 2.3084122243387866e-23, "score": 78.73269653320312, "start": 22, "stop": 173 } ], "seq_stats": { "molecular_weight": 24419.04140000001, "isoelectric_point": 7.664419746398925 }, "id": "IGIGPGHMMC_1346", "locus": "GCA000148625_01336" }, { "type": "cds", "sequence": "contig_1", "start": 767493, "stop": 767630, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTAGATAAAAGAAGAGAAGATACACACCAAGAAGCACAAGAAATAATAGATGCAATTCAAGAAGACAATAGTCAAAATAATACTGTTGAAAATGATCCAAAAGAAGAACAAGAAGACAATAAAAAAGAATAA", "aa": "MILDKRREDTHQEAQEIIDAIQEDNSQNNTVENDPKEEQEDNKKE", "aa_hexdigest": "81dcc8efedbbd55ba062378f701c432f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5368.595700000001, "isoelectric_point": 4.316603279113769 }, "id": "IGIGPGHMMC_1347", "locus": "GCA000148625_01337" }, { "type": "cds", "sequence": "contig_1", "start": 767755, "stop": 768540, "strand": "+", "frame": 1, "gene": null, "product": "Schlafen AlbA-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5", "UniRef:UniRef90_C4XFG5" ], "nt": "ATGAAATTTGTTGAAACAAATAAAATTGAATTAAAGGAAAAATTAAATGATAACTTAATTAGAGAAATTGTTGGCTTTTTAAACACAGAAGGCGGAACAATTTATATAGGTGTAAAAGATAATGGTGAAGTAATTGGCATAAAAGATGTAAATAAAGCTTTTACTGAATTAAACGAAATTATTTGTGATCAAATTGAACCAAAAGCGGAAAAAGAAGTAAAAAGTGAAATAAAATTTGAAAATAACAAAATGATTATGGGAATAAACGTTCAAAAAGGAGAAAAAGCTATTTATTACATAAAAAAATATGGTTGTTCTTCAAAAGGATGTTTAGTAAGAGTTGGGACAAGCACAAGAGAAATGTCAAATGAAGAAATAGAAGAAAGAAACAAAATTAGATTTTATGATGAGGACAACATAATAAATTATGTTAGTGATTTGGATGATTTGAAATTTTCAATATTAAAAATAAATTATGAACTAAATAATTTACATTTAAGAAATAAATTAGGTAGTTACAATAAGCTAGCTGAATTGCTAGCTAATGAAAATAAAATTGACTTGAAATATGTAAAATTTCAAGGAAATGATAAAACAGTCTTTTCAGAAATACAAAAATTCAGTAATAAAAGTATATTGGTTGGTTATTCACAAATTTTAGCAAGATTAGAAGTTGAAAATAAATGCTATGTAGATACTAGTTCAATGCAAAGAATTGAAAAGACTTTATTTGATTTTAATGTAGCTAAAGAGGCTTTATTAAATGCAATTGTACATAATGATTGA", "aa": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFTELNEIICDQIEPKAEKEVKSEIKFENNKMIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHND", "aa_hexdigest": "883478eaf8069c711d95d6540ed33861", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 767755, "stop": 768843, "observations": { "mutation": [ 768538 ], "stop": [ 768538 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG5", "query_cov": 1.0421455938697317, "subject_cov": 0.6126126126126126, "identity": 0.956, "score": 494.0, "evalue": 1.69e-173, "gene_start": 1, "gene_end": 261, "reference_start": 1, "reference_end": 272, "reference_sequence": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFTELNEIICDQIEPRAEKEVKSEIKFENNKMIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLDDKTFEKNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHNDWLSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGKWSVLGIKPEN" }, "paralog": false, "identity": 0.9651474530831099, "score": 702.0, "evalue": 4.95e-258, "description": "Internal stop codon at 768538. Nonsense mutation around 768538.", "psc": { "uniref90_id": "UniRef90_C4XFG5", "product": "Schlafen AlbA-2 domain-containing protein", "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG5", "UniRef:UniRef50_C4XFG5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5" ], "product": "Schlafen AlbA-2 domain-containing protein" } }, "id": "IGIGPGHMMC_1348", "locus": "GCA000148625_01338" }, { "type": "cds", "sequence": "contig_1", "start": 768541, "stop": 769026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13749.12", "PFAM:PF08279.18", "PFAM:PF08220.18" ], "nt": "TTGAGTGGCGTACCAACTATCCAACAATTTGAAAATAGAATTGAAATTATGTCTTATGGTGGATTACCAAAAGGTTTGAAACTTGAAGAATTTTATCAAGGAATAAGTAAGCCAAGAAATCCAAAATTAATGAAAATATTTATAGATCTTAATTTGGCAGAACAAACCGGACATGGCGTTCCACTTATTCTAAAAAAATATGGAAAAATGGCAATTGAAGTAGAAAAAAACTATATCAGGATAACAATACCTTTTAACAAGGAATTGCTACAAAAAAATAACAATTATGAAATTAAAGAAATAAAAGACAAATTAAACAAAATCGAAAATCAAATATTCGAATTAATTCAAAAAAATGAAGAATATACAGCTTATGAATTGTCAAACGAATTAGAAGTTTCTAAAAGAACAATAGAAAGAGCTTTAGCATCTTTAAAAAACAAACTAATTTTAAAAAGAATTGGTTCAAGAAAAACAGGAAAATGA", "aa": "MSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGK", "aa_hexdigest": "664f051e93aff4d5ed5fb5b280f5b65a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13749.12", "name": "HATPase_c_4", "length": 66, "aa_cov": 0.39751552795031053, "hmm_cov": 1.0, "evalue": 1.99113165617746e-15, "score": 52.635982513427734, "start": 6, "stop": 69 }, { "id": "PF08279.18", "name": "HTH_11", "length": 41, "aa_cov": 0.2484472049689441, "hmm_cov": 1.0, "evalue": 3.720441917064276e-09, "score": 32.40493392944336, "start": 110, "stop": 149 }, { "id": "PF08220.18", "name": "HTH_DeoR", "length": 45, "aa_cov": 0.2795031055900621, "hmm_cov": 1.0, "evalue": 1.25345857937012e-06, "score": 24.176462173461914, "start": 110, "stop": 154 } ], "seq_stats": { "molecular_weight": 18713.68890000001, "isoelectric_point": 9.579115486145017 }, "id": "IGIGPGHMMC_1349", "locus": "GCA000148625_01339" }, { "type": "cds", "sequence": "contig_1", "start": 769078, "stop": 769341, "strand": "+", "frame": 1, "gene": null, "product": "prolyl aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "nt": "ATGTACAAAAAATATTTATATCCAAAAATTGAACCTTATCAAAAAGGTTATTTAAAAGTAGATGATATTTATCAAATTTATTATGAAGTTAGTGGCAATCCACAAGGTCAACCAATAGTTTATGTTCATGGTGGTCCTGGTGGAGGAACTAGTGAAGTATGTAGAAGATATTTTGACCCAAAATACTATAAAATAGTTTTATTTGACCAAAGAGGTTGCGGAAAAAGTACTCCAAGTTTAGAGTTAAAAAATAATACAACTTGA", "aa": "MYKKYLYPKIEPYQKGYLKVDDIYQIYYEVSGNPQGQPIVYVHGGPGGGTSEVCRRYFDPKYYKIVLFDQRGCGKSTPSLELKNNTT", "aa_hexdigest": "3de01bb3ebf32601049827166c585ef4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0017C9CCE2", "query_cov": 0.9770114942528736, "subject_cov": 0.9042553191489362, "identity": 0.612, "score": 122.0, "evalue": 8.64e-35, "valid": false, "product": "alpha/beta fold hydrolase", "uniref50_id": "UniRef50_A0A7V9FP21" }, "pscc": { "uniref50_id": "UniRef50_A0A7V9FP21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "product": "prolyl aminopeptidase" }, "genes": [], "id": "IGIGPGHMMC_1350", "locus": "GCA000148625_01340" }, { "type": "cds", "sequence": "contig_1", "start": 770283, "stop": 770768, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354895.1", "SO:0001217", "UniParc:UPI0001E32F9F", "UniRef:UniRef100_UPI0001E32F9F", "UniRef:UniRef50_C4XFG2", "UniRef:UniRef90_C4XFG2" ], "nt": "GTGAAACTATCGATCAAAGAAATTATCAAGAGAATTAAAATTCTTGAGGACGAATTAGTTGTTCTTAATCAAGAAGAAGATAAAGATAATTATGTTATATATTTAAAAGATGAAACAGTTGAAAAATCATCGTATAATTTTAATAAAACTAATAAAAAACGTCAAGAAATTAATCAAGAAATTTTAAGACTTAAAATTATTGTTCAAAAAGCAAACATCAAAACAATAACTAGTTATAAAGGATTAAGTATTTCTGAACTTTTAATTTTATTAGCACAAAAATCTTCTTTAGTTCAAAGATTTGATTTGTTATGTTCTAAAAAGCAAAAAACAAGAAAAACTACCAACGATGGTCAAATTGAATATATTGAATATTTATATGATATTAACGAATTAAAAAATACTAATTTAAAATTAAAACAAGAAATAGCAACAATGCAAGTTGCTATTGATAAAGCTAATATTGAAACATTAATTGAAATATAA", "aa": "MKLSIKEIIKRIKILEDELVVLNQEEDKDNYVIYLKDETVEKSSYNFNKTNKKRQEINQEILRLKIIVQKANIKTITSYKGLSISELLILLAQKSSLVQRFDLLCSKKQKTRKTTNDGQIEYIEYLYDINELKNTNLKLKQEIATMQVAIDKANIETLIEI", "aa_hexdigest": "fb176cb0cce066edf3f470c08d00fb06", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F9F", "ncbi_nrp_id": "WP_013354895.1", "uniref100_id": "UniRef100_UPI0001E32F9F", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F9F", "RefSeq:WP_013354895.1", "UniRef:UniRef100_UPI0001E32F9F" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F9F", "uniref90_id": "UniRef90_C4XFG2" }, "psc": { "uniref90_id": "UniRef90_C4XFG2", "uniref50_id": "UniRef50_C4XFG2" }, "pscc": { "uniref50_id": "UniRef50_C4XFG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18896.811000000005, "isoelectric_point": 8.96382923126221 }, "id": "IGIGPGHMMC_1351", "locus": "GCA000148625_01341" }, { "type": "cds", "sequence": "contig_1", "start": 770872, "stop": 771078, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAATCTGCGATAATTTAAGCATGACAACAAATACTAATAAAAATTCTTTAACAACAACCATTCAATTAATATTAGAAGATGGGACATTAGACGGGATTTTGTATCTCAAAACTTTAAGATGGCCTCTAGGAGGAATTATGTTGGTAAGTCCTCGCGATAAGGTTACTAAATTATTGGACGAATATGAAGAATGTAATAATTGA", "aa": "MLICDNLSMTTNTNKNSLTTTIQLILEDGTLDGILYLKTLRWPLGGIMLVSPRDKVTKLLDEYEECNN", "aa_hexdigest": "991e593e6f90afe58f95af4846ab850a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7688.845199999999, "isoelectric_point": 4.563057136535646 }, "id": "IGIGPGHMMC_1352", "locus": "GCA000148625_01342" }, { "type": "cds", "sequence": "contig_1", "start": 771439, "stop": 771849, "strand": "+", "frame": 1, "gene": null, "product": "GIY-YIG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG1", "UniRef:UniRef90_C4XFG1" ], "nt": "GTGAATTTATCAAATATTATTAAACCCAAATCAGAAGAAGAAATTAGAAGTCGAAGTAAACAAGAAATATTTTCTTATATTGAAACAAAAACAAAAATTGATTTAAAAAATAATACTTACTATAGTAAATATTATGATCATAAAAATTGTTATTGGTTTACTTTAAAAACTAATTCAGTTAATAGTGATTTAAAATTAGTACTAAATGATATAAATTCTCAAGAAATAACAATTTTGCAAATTCCAGCCAAAAAATTTGAAATTTCAGATACTAAAGGAACGAACAAATTTCAAAAAACTAGAAGCGATAATAAATTTGATTTATATATAAATAATGTTTTATTCGAAAAACAAAATAAAATTGACTTTACAGAATATGTAATAGATAAAATTCATTATTCTTCCTCATAA", "aa": "MNLSNIIKPKSEEEIRSRSKQEIFSYIETKTKIDLKNNTYYSKYYDHKNCYWFTLKTNSVNSDLKLVLNDINSQEITILQIPAKKFEISDTKGTNKFQKTRSDNKFDLYINNVLFEKQNKIDFTEYVIDKIHYSSS", "aa_hexdigest": "e928619564e748230e31fdfc60699184", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 771139, "stop": 771849, "observations": { "mutation": [ 771172, 771175 ], "stop": [ 771172, 771175 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG1", "query_cov": 1.0, "subject_cov": 0.4035608308605341, "identity": 0.963, "score": 249.0, "evalue": 8.24e-81, "gene_start": 1, "gene_end": 136, "reference_start": 202, "reference_end": 337, "reference_sequence": "MKVESYKKSNIFLLICDNLSMTTNTNKNSLTTTIQLILEDGTLDGILYLKTLRWPLGGIMLVSPRDKVTKLLDEYEECNNWGIYLLIAKDQVYVGQSSELKQRIEQHLLGKDWWEKVFLFTTSNNSLDKSGIDYLESKLIDKAKANNKLSSDNKKNGNKTNIDRFRETELKEYLNELIFLLDFINIDVFNDNTNKKNNLENVNLSNIIKPKSEEEIRSRSKQEIFSYIETKTKIDLKNNTYYSKYYDRKNYYWFTLKNNSVNSDLKLVLNDINSQEITILQIPAKKFEISDTKGTNKFQKTRSDNKFDLYINNVLFEKQNKIDFTEYVIDKIRYSSS" }, "paralog": false, "identity": 0.9745762711864406, "score": 453.0, "evalue": 2.19e-163, "description": "Internal stop codon at 771172, 771175. Nonsense mutation around 771172, 771175.", "psc": { "uniref90_id": "UniRef90_C4XFG1", "product": "GIY-YIG domain-containing protein", "uniref50_id": "UniRef50_C4XFG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG1", "UniRef:UniRef50_C4XFG1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG1" ], "product": "GIY-YIG domain-containing protein" } }, "id": "IGIGPGHMMC_1353", "locus": "GCA000148625_01343" }, { "type": "cds", "sequence": "contig_1", "start": 771916, "stop": 772146, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1", "UniRef:UniRef90_UPI0001E32FA1" ], "nt": "GTGGTATTTACTAAGGATTTAAATGTTTATGTTCATTTTGCTTTTATTATTTTAGCTATCTTATTCTTAATCTTTGCAACAGTAATTATTAATTATGTTTTCTATAAATATGTTGTTGGTTTACATAAAATATTTAGCGATAAAGATAAAAACAATTCTGATATTCAAAATCAAAAAACAATTGTTGAAGGAATTGAAGTTACTAAAGTTGACTTAGATTCTTTAGAGTAA", "aa": "MVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE", "aa_hexdigest": "76c01552a3dd2aa9b2239952e9e571be", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 771916, "stop": 772389, "observations": { "mutation": [ 772206, 772266 ], "stop": [ 772206, 772266 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FA1", "query_cov": 0.9868421052631579, "subject_cov": 0.47770700636942676, "identity": 1.0, "score": 138.0, "evalue": 2.94e-40, "gene_start": 2, "gene_end": 76, "reference_start": 83, "reference_end": 157, "reference_sequence": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVGWAAYFYSLAISIKSKKFYFTWACFTFPIAISSTALFKYAQVVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE" }, "paralog": false, "identity": 0.9872611464968153, "score": 302.0, "evalue": 2.34e-107, "description": "Internal stop codon at 772206, 772266. Nonsense mutation around 772206, 772266.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FA1", "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FA1", "UniRef:UniRef50_UPI0001E32FA1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8816.216200000003, "isoelectric_point": 5.5337533950805655 }, "id": "IGIGPGHMMC_1354", "locus": "GCA000148625_01344" }, { "type": "cds", "sequence": "contig_1", "start": 772264, "stop": 772389, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTAAGTTTTGCATTTTTAACTTTATTTGATCCTAAGAATCCAAACGCAAATATTTTAAATAATTATGTAATTTTATATACTTTTACAGGAATATTATTCTTATTTATTTTAGTTGGTTGA", "aa": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVG", "aa_hexdigest": "f9947ede4f3897ac5b704ebd3b555576", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4668.535199999998, "isoelectric_point": 5.587011528015137 }, "id": "IGIGPGHMMC_1355", "locus": "GCA000148625_01345" }, { "type": "cds", "sequence": "contig_1", "start": 772596, "stop": 773078, "strand": "-", "frame": 3, "gene": null, "product": "Voltage-dependent anion channel", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA2", "UniRef:UniRef90_UPI0001E32FA2" ], "nt": "ATGACTTTTAAAAATAAATTAGAAAAATTGCCTCTTGGTTTAGGAGGACTTGCACTTGGTATGAGTGGTTTAGCTGGTGCTTATAATGGAGCTTTTAATGAGTTTAAAAACTCAAGCAATGAAGAATTAATTAATAATGTTTCAATTGGCATTCAGGCTTTTTGGATGTTAATTGCTTTTGCTTTAGCTTTTATTGTTTGCTTAAGATTTATTAAAGCAAGACATGTATTTAATAAAGAAGCAAAAGCTCCAAATACAGCAGCATTTATACCTACTTTATTAATGACATTTATTTCTATTTTTAATTTTATAGCCTCAATATTAAATACTTATTTAGAAGCTAGTGAAGCTAAAAAATGGTTATATATTCCATTGGTATTTTCATTCATTGCTTTTATATTACAAATAGCTTATTTAATAACTTTTATAGTATTAATTATAGTTCGACATGATTTTAAATATGATGAAGCTTTTGCTTCTTGA", "aa": "MTFKNKLEKLPLGLGGLALGMSGLAGAYNGAFNEFKNSSNEELINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFNFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVRHDFKYDEAFAS", "aa_hexdigest": "309dca31eab9f346a42d6b395ecd74c5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 772467, "stop": 773078, "observations": { "mutation": [ 772508, 772598 ], "stop": [ 772508, 772598 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FA2", "query_cov": 1.0, "subject_cov": 0.7881773399014779, "identity": 1.0, "score": 277.0, "evalue": 3.21e-93, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MTFKNKLEKLPLGLGGLALGMSGLAGAYNGAFNEFKNSSNEELINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFNFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVRHDFKYDEAFASWFIPTVGFIILTFGANNYQNIIPLLFYQILWFIGLLFCFLTFF" }, "paralog": false, "identity": 0.9901477832512315, "score": 366.0, "evalue": 1.49e-130, "description": "Internal stop codon at 772508, 772598. Nonsense mutation around 772508, 772598.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FA2", "product": "Voltage-dependent anion channel", "uniref50_id": "UniRef50_UPI0001E32FA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FA2", "UniRef:UniRef50_UPI0001E32FA2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA2" ] } }, "id": "IGIGPGHMMC_1356", "locus": "GCA000148625_01346" }, { "type": "cds", "sequence": "contig_1", "start": 773149, "stop": 773376, "strand": "+", "frame": 1, "gene": null, "product": "DNA (cytosine-5-)-methyltransferase N-terminal subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4K8", "UniRef:UniRef90_UPI00031A3560" ], "nt": "ATGAAAAAATATGTAAAATTATTTGAATTTTTTGCTGGTATTGGAAGTCAATATCAAGCATTGAAAAATCTAGAAAATGAAATGAATTTTAAAACAATTTCACTTGGCACATGTGATTTTTTTATTCCATCAATAATTAGTTACATGATTATTCATCATGGACTTTTAAAACCAGAAAATGAATTGTCCAAAACTGAAATGATTAAAATTTTAAAACAATTTTCATGA", "aa": "MKKYVKLFEFFAGIGSQYQALKNLENEMNFKTISLGTCDFFIPSIISYMIIHHGLLKPENELSKTEMIKILKQFS", "aa_hexdigest": "ee5a96ee1ba5e149d04a1eab8440800d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 773149, "stop": 773550, "observations": { "mutation": [ 773374 ], "stop": [ 773374 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00031A3560", "query_cov": 1.0, "subject_cov": 0.5639097744360902, "identity": 1.0, "score": 152.0, "evalue": 3.16e-46, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "MKKYVKLFEFFAGIGSQYQALKNLENEMNFKTISLGTCDFFIPSIISYMIIHHGLLKPENELSKTEMIKILKQFSWSKDSKKLVADNYFEKMSESKLKSIFPYLFAYVDNEYFAKKYQFSIDQRERERERTIA" }, "paralog": false, "identity": 0.9924812030075187, "score": 263.0, "evalue": 3.22e-92, "description": "Internal stop codon at 773374. Nonsense mutation around 773374.", "psc": { "uniref90_id": "UniRef90_UPI00031A3560", "product": "DNA (cytosine-5-)-methyltransferase N-terminal subunit", "uniref50_id": "UniRef50_A0A449B4K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00031A3560", "UniRef:UniRef50_A0A449B4K8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B4K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4K8" ], "product": "DNA (cytosine-5-)-methyltransferase" } }, "id": "IGIGPGHMMC_1357", "locus": "GCA000148625_01347" }, { "type": "cds", "sequence": "contig_1", "start": 773422, "stop": 773550, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAAGCAAACTTAAAAGCATTTTTCCTTATCTATTTGCATATGTTGACAATGAATACTTTGCTAAAAAATATCAATTTTCAATAGATCAGAGAGAGAGAGAGAGAGAGAGAACTATCGCTTAA", "aa": "MSESKLKSIFPYLFAYVDNEYFAKKYQFSIDQRERERERTIA", "aa_hexdigest": "3c1bfa855a5b0aa8b0c12aa04404cc4b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5198.8181, "isoelectric_point": 8.034453010559083 }, "id": "IGIGPGHMMC_1358", "locus": "GCA000148625_01348" }, { "type": "cds", "sequence": "contig_1", "start": 773601, "stop": 773798, "strand": "+", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002804CCAA" ], "nt": "ATGACATATTCTTTCCCTTGTCAAGATTTGTCGCAACAAGGAAAACAACGAGGAATACATGCAAACACAAGAAGTGGTTTGCTTTTTCAAATTGAAAGAATACTTAAAAATAATACAGATAAATTACCCAAAATTTTACTTTTAGAAAATGTAAAAGCTTTAGTATCTAATAAATTTATAGACAGTTTACATAGCTGA", "aa": "MTYSFPCQDLSQQGKQRGIHANTRSGLLFQIERILKNNTDKLPKILLLENVKALVSNKFIDSLHS", "aa_hexdigest": "84eeaf1b7b279c318021f3a9e552594c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002804CCAA", "query_cov": 0.9384615384615385, "subject_cov": 0.8243243243243243, "identity": 0.8029999999999999, "score": 100.0, "evalue": 9.71e-27, "valid": false, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI002804CCAA" }, "pscc": { "uniref50_id": "UniRef50_UPI002804CCAA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002804CCAA" ], "product": "DNA cytosine methyltransferase" }, "genes": [], "id": "IGIGPGHMMC_1359", "locus": "GCA000148625_01349" }, { "type": "cds", "sequence": "contig_1", "start": 773841, "stop": 774368, "strand": "+", "frame": 3, "gene": null, "product": "DNA (cytosine-5-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A8D4A565" ], "nt": "GTGGTTAATGCTACTGAATATGGTAGCTGTCAAAATCGCGAAAGAGTTTTTATGGTTTCAATTTTAAACAATAATAAACCATTTGTTTTCCCTAAAAAGTCTATAAAACAGAAAGATTTATCAAAAATTATACTGCCAGAAAATGAAGGAAAAAGTTTTCAACATTTGCTCAAATATGAAAAATTACCTTTTAATAAAACCAAAAGTCAAATTACGAAAAGTAAATTGTTAAATTACACATCGTTCAATTCTGAAGCTTATATTTATGTACCTATTGGTTTTGGCCCTACATTAACAGCTTCTGGAGCAAATAGCCGTTTGAAATTTTATTTTGAAAAAGAAAATATATTAAGAAGCATTAACGCGATTGAAACTTATCAATATATGGGCTTTGAAATTAAGAATGCTAAAGATATTATTAAATCAAAATTAGTTACAGAAAATGAAATGATTTTTACTTGTGGAAATAGCATAAGTGTTGAAGCGCTTGAAGCAATTTTTAGAGAGGCTATAAATGAGTACTTATAA", "aa": "MVNATEYGSCQNRERVFMVSILNNNKPFVFPKKSIKQKDLSKIILPENEGKSFQHLLKYEKLPFNKTKSQITKSKLLNYTSFNSEAYIYVPIGFGPTLTASGANSRLKFYFEKENILRSINAIETYQYMGFEIKNAKDIIKSKLVTENEMIFTCGNSISVEALEAIFREAINEYL", "aa_hexdigest": "5ce853187843cf1aff9395bba66cdf8c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028062644", "query_cov": 1.0171428571428571, "subject_cov": 0.8682926829268293, "identity": 0.5670000000000001, "score": 191.0, "evalue": 7.12e-59, "valid": false, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_A0A8D4A565" }, "pscc": { "uniref50_id": "UniRef50_A0A8D4A565", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A8D4A565" ], "product": "DNA (cytosine-5-)-methyltransferase" }, "genes": [], "id": "IGIGPGHMMC_1360", "locus": "GCA000148625_01350" }, { "type": "cds", "sequence": "contig_1", "start": 774595, "stop": 775239, "strand": "+", "frame": 1, "gene": null, "product": "MAG4270 family putative restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA3", "UniRef:UniRef90_UPI0001E32FA3" ], "nt": "TTGATAAGAGAAAAACTTTTTAAAAGATTGAATATTGAACAATTGCCAAGACCATCTATTAAAGACAATATGATAAAACATTTAAACAAAGAAGAAGTTAATATTGTAAATAATTTTATTAATGAAAATTATGATTCTACATTAAAGAACTTAACTGCTTTAATAAAGGGAAATAATCCAACTGGTAGTTTATTAAAACCAGAAGCATCTGAAATAATAGCATTTAATAATGAAAAAAGAATTTATAACTTAATTATGTTTCTAGACAGAATATCATTAATAAAAAAGAGAAAAGATTTAATGACATTAAAAGATATTTTTAACATAGAACAAGAAGATTTTATAAATGCTTTATATAATGCTGAAAATAAAAACATTATTTTAAAAGTAGAAGACTGTATTAATTTATACACAAAAGATGCTGATGACTATTTCAAAAATTATTTTGTTAAATTAAATAATTCTGATTATTATGAAGGCAAAAGAATAACAAATTCACAAAGCATTAAAAGAGCAAAAGAAATCATTAATAAATTTGCTAAGAAAATTTCACTTACATATAAAATTGACCAAATTTGTAAAGAAGAAGAAGGAAAGTACCACAAACATAAGATAGATTTTAATGCAACTTTTAATAATTGCTGA", "aa": "MIREKLFKRLNIEQLPRPSIKDNMIKHLNKEEVNIVNNFINENYDSTLKNLTALIKGNNPTGSLLKPEASEIIAFNNEKRIYNLIMFLDRISLIKKRKDLMTLKDIFNIEQEDFINALYNAENKNIILKVEDCINLYTKDADDYFKNYFVKLNNSDYYEGKRITNSQSIKRAKEIINKFAKKISLTYKIDQICKEEEGKYHKHKIDFNATFNNC", "aa_hexdigest": "a28ba6e9d3daf057c96ebc92d9070e1a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 774355, "stop": 775542, "observations": { "mutation": [ 774460, 774517, 774568, 775237 ], "stop": [ 774460, 774517, 774568, 775237 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FA3", "query_cov": 1.0, "subject_cov": 0.5059101654846335, "identity": 0.995, "score": 419.0, "evalue": 7.62e-145, "gene_start": 1, "gene_end": 214, "reference_start": 81, "reference_end": 294, "reference_sequence": "MSTYKEKSDYILSEYKIYKIPFTSKNYLKKESKNKWFFKGNIYVFIGIDNLVYNWIVKFDEISLDCFYTPIWKENKNHNYLIREKLFKRLNIEQLPRPSIKDNMIKHLNKEEVNIVNNFINENYDSTLKNLTALIKGNNPTGSLLKPEASEIIAFNNEKRIYNLIMFLDRISLIKKRKDLMTLKDIFNIEQEDFINALYNAENKNIILKVEDCINLYTKDADDYFKNYFVKLNNSDYYEGKRITNSQSIKRAKEIINKFAKKISLTYKIDQICKEEEGKYHKHKIDFNATFNNCWDKMEEAAHIYNRKDIKKEMVKEAIKYDVFSDDKNSDKHLKIILNNIKIIGKQISDPNNLLNLDKTMHGYFDDNYFTYDENGKLISLKDNIKFENFLNKFDYYKQIPLQKLNENRKKYIKLRNENIKNK" }, "paralog": false, "identity": 0.9898734177215189, "score": 786.0, "evalue": 4.42e-292, "description": "Internal stop codon at 774460, 774517, 774568, 775237. Nonsense mutation around 774460, 774517, 774568, 775237.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FA3", "product": "MAG4270 family putative restriction endonuclease", "uniref50_id": "UniRef50_UPI0001E32FA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FA3", "UniRef:UniRef50_UPI0001E32FA3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA3" ], "product": "MAG4270 family putative restriction endonuclease" } }, "id": "IGIGPGHMMC_1361", "locus": "GCA000148625_01351" }, { "type": "cds", "sequence": "contig_1", "start": 775246, "stop": 775626, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13391.12" ], "nt": "ATGGAAGAAGCTGCTCATATATATAATAGAAAAGATATCAAAAAGGAAATGGTCAAAGAAGCTATTAAATATGATGTTTTTTCAGATGACAAAAACAGTGACAAACACTTAAAAATAATTTTGAATAATATAAAGATAATTGGAAAACAAATCAGTGATCCAAATAATTTACTAAACTTAGATAAAACAATGCATGGATATTTTGATGACAACTATTTTACGTATGATGAAAATGGAAAATTAATATCTTTAAAAGATAATATTAAATTTGAAAATTTTTTAAATAAATTTGATTATTACAAACAAATACCACTGCAAAAATTAAATGAAAACAGAAAAAAATACATTAAATTAAGAAACGAAAACATAAAAAATAAATAA", "aa": "MEEAAHIYNRKDIKKEMVKEAIKYDVFSDDKNSDKHLKIILNNIKIIGKQISDPNNLLNLDKTMHGYFDDNYFTYDENGKLISLKDNIKFENFLNKFDYYKQIPLQKLNENRKKYIKLRNENIKNK", "aa_hexdigest": "3aac7c12c9baba1ab2d6e047b8aab464", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13391.12", "name": "HNH_2", "length": 79, "aa_cov": 0.5714285714285714, "hmm_cov": 0.6582278481012658, "evalue": 4.500123104913985e-06, "score": 22.507810592651367, "start": 3, "stop": 74 } ], "seq_stats": { "molecular_weight": 15194.226899999985, "isoelectric_point": 9.18759899139404 }, "id": "IGIGPGHMMC_1362", "locus": "GCA000148625_01352" }, { "type": "cds", "sequence": "contig_1", "start": 776576, "stop": 776926, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "6a907dccef0cbbba0bbf260e0f56003e", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13576.067399999976, "isoelectric_point": 9.912288093566893 }, "id": "IGIGPGHMMC_1363", "locus": "GCA000148625_01353" }, { "type": "cds", "sequence": "contig_1", "start": 777146, "stop": 778243, "strand": "-", "frame": 1, "gene": null, "product": "Dihydrolipoyl dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "nt": "ATGCATGAACGTAAAAAAGGCGTAGTAGATCAAATTTCAAGTAGTGTTAAAAAATTAATGATTGCTTCTAAATGTAAAATAGAAGAAGGCGAAGCAGAATTTGTTGGAGCTCACGAAATTAAAATAAATGGTAAAGTATATCGTGGCACAAATATTATTTTAGCCACAGGCAGTCACTCACGTAGATTGCGTGCTTTACCTGGATTTAAGGTTGGATATGAAAATAATTATGTTCTTTCAAGCCGCGAAGCAATTAACTATGATTCAAAATTGCCTGGATCGGTAGTTATTGTTGGTGGTGGTGTTGTTGGCGTTGAATTTGCTCAAGTATTTGCTTCAGCAGGTGCTAAAGTAACTATTATTCAAAACCAAAACCACTTACTTCCTGGTATTGATCATGATGTAACAAATGAAATTCTTAAACATCTAGAAAAGCATGGGGTTCAAATTATTTACAATGCAACTTCAACAGGTTTAAATAATAAAAAAGAACTTCTTTACGAAATTGGTGGTAAAGAAAGAAAAATTAAAGCTGATGTTTATTTGATTGCTGTTGGACGTATTCCATCAAGTAAAGGTATTGCAGAAGTTGGAGTTAATGTCGGAGTTAGAGAGGAAGTTCTAGTTGATGAAAAAATGAGAACAAATGTTAAAGGCGTTTATGCAATTGGAGATTTAACTGGACAAAACATGTTAGCTCACGTTGCATATCAACATGCTTTAATTGCTGTTGGTAACATTTTAGGTGAAAAAAATGTTCGTTATCACAATAAACCAGTTCCAGGATGTATTTATACAAACCCTGAAATTGCTTTCATTGGTTTAACTGAAGAAGAAGCAAAAAATAAAGGATACAATATTTTTACTTCAAAATACATGTTCTCTTATTTAGGAAAAGCTATTGCAACCAAACAAACTTCTGGTTTTGTTAAATTAGTTGTTGATCGTGAATATGGGCAAATATTAGGAGCTCACATTATTGGAGCTCATGCAACTGACTATATTTCGGAAATAGCACTTGCTATGGAACAAGAAGTTAGTGTCAAAGAATTAGCTTACACAATTCACCCACACCCAACTTATTCGGAAATTATTTGA", "aa": "MHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNPEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEII", "aa_hexdigest": "d8f4e719c5e8344aab4ebb095ab15cdc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A223MB31", "query_cov": 0.8602739726027397, "subject_cov": 0.9661538461538461, "identity": 0.513, "score": 315.0, "evalue": 4.05e-103, "valid": false, "product": "Dihydrolipoyl dehydrogenase", "uniref50_id": "UniRef50_A0A223MB31" }, "pscc": { "uniref50_id": "UniRef50_A0A223MB31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [], "id": "IGIGPGHMMC_1364", "locus": "GCA000148625_01354" }, { "type": "cds", "sequence": "contig_1", "start": 778250, "stop": 778351, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATCAACTGATGTTTTAGAAACCTTAACCGATGCTGCTGGTTATGGTATTGTTGGGAACTTAGATAAATTAAAGATAGATCTACAAAAAACATGA", "aa": "MLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKT", "aa_hexdigest": "f965803749b04d3a897180f271242a87", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3565.094500000001, "isoelectric_point": 4.777965354919433 }, "id": "IGIGPGHMMC_1365", "locus": "GCA000148625_01355" }, { "type": "cds", "sequence": "contig_1", "start": 778394, "stop": 779302, "strand": "-", "frame": 1, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "EC:1.8.1.4", "GO:0004148", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_A0A449B5U3", "UniRef:UniRef90_C4XFF7" ], "nt": "ATGTACGTATTTAAATTTGCTGATATAGGTGAAGGATTACACGAAGGAACAGTTGGTGAAATCAGTGTTAAAGTTGGCGACAAGATTAAAGAAGGAGATACACTTTTTTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAGAATGAAGACTGGTGATGTAATTCACGTTGGCCAAGAAATATTTGTAATTGATGATGGCAAAGGTGGCTCAGTAGCTGCAGCTCCTGCTACTAAAGTTGAAGAATCGAAAAGTACACCTGCTACTTCATCATCTACAAGCTTTTATGTTTTTAAATTTGCTGACATTGGTGAAGGTTTACACGAAGGAACAGTTGGTGAAATTCCTATTAAAATAGGTGATAAAGTAAAAGAAGGCGACACATTATTCTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAAAATGAAAACAGGTGATGTAATTCATGTAGGACAAGAAGTTATTGTTATTGATGCAGGAAGTAAAAATCCTACTTCTTCTTCAGTTTCATCTGCACCAAGTTCCAATAAAGAAGAAGAAAAATGTTTAGTAGGTGAAGCTCCAAATTCAAGTGAATTAATAAATCTAGATTTCAGTTCACTAAATTCACCCGCAAAAGAAACTCCTCAACCAGTTGCACCTCAAGCTTCAACTTATGAAGCTAACCAAGAAGAAGAAAGCAATGGTGTTTCATTTACTTCTGAAGGTAAAAAATACGAAGGCAAAGTTGATGAAGAATTTGATGTAATTATTGTAGGTAGTGGTCCTGGAGGATATTTAGCCGCTGAAGAATTAGGCAAAGCAGGCAAAAAAGTATTAATTGTTGAAAAAGAATTCTGA", "aa": "MYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSTPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGEAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEF", "aa_hexdigest": "22d466d217d49682fa1a8f4d5f847586", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "lpdA" ], "truncated": "5-prime", "pseudogene": { "start": 778091, "stop": 779308, "observations": { "mutation": [ 778252, 778396 ], "stop": [ 778252, 778396 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF7", "query_cov": 1.0, "subject_cov": 0.4092140921409214, "identity": 0.993, "score": 507.0, "evalue": 9.26e-174, "gene_start": 1, "gene_end": 302, "reference_start": 3, "reference_end": 304, "reference_sequence": "MNMYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSRPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGDAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEFWGGVCLNIWCIPTKAMLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKTWVKMHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNTEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEIIWEAARAASLKLYLEENKK" }, "paralog": false, "identity": 0.9851851851851852, "score": 716.0, "evalue": 3.01e-258, "description": "Internal stop codon at 778252, 778396. Nonsense mutation around 778252, 778396.", "psc": { "uniref90_id": "UniRef90_C4XFF7", "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_A0A449B5U3", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF7", "EC:1.8.1.4", "UniRef:UniRef50_A0A449B5U3", "KEGG:K00382", "GO:0004148", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5U3" ], "product": "Dihydrolipoyl dehydrogenase" } }, "id": "IGIGPGHMMC_1366", "locus": "GCA000148625_01356" }, { "type": "cds", "sequence": "contig_1", "start": 779385, "stop": 779822, "strand": "-", "frame": 3, "gene": null, "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197", "UniRef:UniRef90_C4XFF6" ], "nt": "TTGCCTTATATTATTAAAGCAGCTGCTATTGCTTTAAGAGATTTTCCAGTATTCACTGCTAAATATAATGAAGCAAATCAAACTCTAGATTACCCTGGTGTAATTAATGTTGGTATTGCTGTTGATACTGAAGCTGGTTTAATGGTTCCTGTTATTAATAATGCTGATAATTTAAGTATTGTTAATATTGCTAACGAAGTAACTAGACTTGCAGGCGCTGCAAGAAATAGAACAATTAAACCTGCTGAAATGAAAGGTGCCGGTTTTACTATTACTAACTATGGTTCAGTTGGATCACTTTTTGGAGTTCCTGTTATTAACTATCCAGAATTAGCTATTGGTGGTGTTGGTGCTATTATTGACAAGCCTGTAGTTAAAAATGGACAAGTTGTACCTGGAAAAGTTATGTACCTAACTGTCGCTGCTGATCACCGTTGA", "aa": "MPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIANEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHR", "aa_hexdigest": "674dfc23769c38070ad07bea3d5f4078", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 779304, "stop": 780122, "observations": { "mutation": [ 779387, 779885, 779939 ], "stop": [ 779387, 779885, 779939 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF6", "query_cov": 1.0, "subject_cov": 0.45741324921135645, "identity": 0.986, "score": 278.0, "evalue": 2.69e-92, "gene_start": 1, "gene_end": 145, "reference_start": 146, "reference_end": 290, "reference_sequence": "MMKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISGAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNSWSNVAYTNLVHEIDMTALWDLRSRIKDIVLKSDNVKITFLPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIASEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHRWIDGAVIGRFASRIKELLEKPDVLGVY" }, "paralog": false, "identity": 0.9816176470588235, "score": 512.0, "evalue": 1.12e-186, "description": "Internal stop codon at 779387, 779885, 779939. Nonsense mutation around 779387, 779885, 779939.", "psc": { "uniref90_id": "UniRef90_C4XFF6", "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF6", "UniRef:UniRef50_A0A449B197" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197" ], "product": "Pyruvate dehydrogenase E2 component (Dihydrolipoamide acetyltransferase)" } }, "id": "IGIGPGHMMC_1367", "locus": "GCA000148625_01357" }, { "type": "cds", "sequence": "contig_1", "start": 779937, "stop": 780254, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAATCAACCCCTATTGCAAGAGCTATGGCTGCTAAACTTGGAATTGATATTAACTTAGTTCCAGGTTCAGGAATTGATGGCCGTATTCTTATAGAAGATATTCAAAAATTTAAAAACAGTCCTATTTCATGTGCTGCTCATTCAAACTTTGCACCAGCTCCAGTAGCTGATAATAAAGTAATTACTCAACCTAGTGCAGTAGCTCCTAAAGCTACTCCAGCACCTCGTCCTGCCATTGATCGTTCAGCTCACTCAGAAGCTATTACTCCAATTAGAAAAGCAATTTCTAAAGCTATGACTAACTCATGA", "aa": "MKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISCAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNS", "aa_hexdigest": "1fcc235c06d503d5c9ebfc69312f1a31", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10950.658299999983, "isoelectric_point": 10.099375343322752 }, "id": "IGIGPGHMMC_1368", "locus": "GCA000148625_01358" }, { "type": "cds", "sequence": "contig_1", "start": 780377, "stop": 780967, "strand": "-", "frame": 1, "gene": null, "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "nt": "ATGATTTTAAGAATGCCTATGGGTGGTGGAATTAAAGCTATGGAACATCACTCAGAAGCTTTAGAAGCTATTTATGCTCACATTCCAGGTGTTAAAGTTGTTATGCCGGCCTTTCCTTATGATGTTAAAGGTTTATTATTAGCAGCTTTAAATGATCCAGATCCAGTAGTATTCTTAGAAAATAAGAAAATTTATCGTGCTGGGAAACAAGAAGTTCCAGCTGGCGAATATACAGTTGAAATTGGAAAAGCTAATGTCTTAACTCAAGGTAATGACTTAACTTTAGTAACTTATGGAGCTCAAGTATTCGACTCAATTAATGCTGTTAAAAAATATAAAGAAATCAATCCTAACGCTTCAATTGAATTAATTGATTTAAGAACAATTAAACCTCTTGATACTAAAACTATTGTTGAAAGTGTTAAGAAAACAGGACGTTTATTAGTAGTTCATGAAGCTGTTAAATCATTTTCAGTTTCTTCTGAAATTATGGCTAGAGTTAATGAAAAAGCATTTGAATATCTTAAAGCACCTATGACAAGATGTACAGGTTATGATGTAACTGTTCCTCTTGCAAAAGGCGAAGCATGA", "aa": "MILRMPMGGGIKAMEHHSEALEAIYAHIPGVKVVMPAFPYDVKGLLLAALNDPDPVVFLENKKIYRAGKQEVPAGEYTVEIGKANVLTQGNDLTLVTYGAQVFDSINAVKKYKEINPNASIELIDLRTIKPLDTKTIVESVKKTGRLLVVHEAVKSFSVSSEIMARVNEKAFEYLKAPMTRCTGYDVTVPLAKGEA", "aa_hexdigest": "d40c9db939a38851a1139fa7c3de90c4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A2H6AHZ3", "query_cov": 0.8520408163265306, "subject_cov": 0.8743455497382199, "identity": 0.503, "score": 167.0, "evalue": 3.5e-49, "valid": false, "product": "Pyruvate dehydrogenase E1 component subunit beta", "uniref50_id": "UniRef50_A0A2H6AHZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6AHZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" }, "genes": [], "id": "IGIGPGHMMC_1369", "locus": "GCA000148625_01359" }, { "type": "cds", "sequence": "contig_1", "start": 781001, "stop": 781315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "ATGGCTGAAGAAAAATTAACTTTAAATAATGTTCAAGCAGTTAATAATGCTTTAGACATTGCTATGGCTAAAGACCCCCGTGTAGTTTGTTATGGTGAAGATGCTGGTGTTGAAGGTGGAGTTTTCAGAGCTACAGAAGGCTTACAAAAAAAATATGGTAAAAGTAGAGTATTTGATACCCCTATTTCAGAAGCAACAATTGCTGGTACCGCAATTGGTGCTGCAGTAGCAGGTCTTAGACCTATTGCCGAAATTCAATTCCAAGGTTTTAGTTATCCAGCTATGCAACAATTATTTACTCATGCAGCTAGATGA", "aa": "MAEEKLTLNNVQAVNNALDIAMAKDPRVVCYGEDAGVEGGVFRATEGLQKKYGKSRVFDTPISEATIAGTAIGAAVAGLRPIAEIQFQGFSYPAMQQLFTHAAR", "aa_hexdigest": "0e2861927209bd36f7e5d66cd7ade891", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 94, "aa_cov": 0.8942307692307693, "hmm_cov": 0.9787234042553191, "evalue": 8.988537449651133e-22, "score": 73.55757904052734, "start": 10, "stop": 102 } ], "seq_stats": { "molecular_weight": 11074.48989999999, "isoelectric_point": 5.746047019958497 }, "id": "IGIGPGHMMC_1370", "locus": "GCA000148625_01360" }, { "type": "cds", "sequence": "contig_1", "start": 781855, "stop": 782022, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCCGGTTAATATTGTTATTGCAACTCAAATTTCACAATGTGCTGGTATTGCTTATGCACAAAAACAACTTAAAACTGGTGGTGTAGCTATTTCATTTATTGGAAATGGCGGAACTACTGAAGGAGAATTTAGCGAAGGTGTTAATATTGCAACTGTTCAAGAATGA", "aa": "MPVNIVIATQISQCAGIAYAQKQLKTGGVAISFIGNGGTTEGEFSEGVNIATVQE", "aa_hexdigest": "d2b0ffa0a9c9fc647b7662acfb9fff92", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 53, "aa_cov": 0.9636363636363636, "hmm_cov": 1.0, "evalue": 2.72570311220023e-12, "score": 42.23418045043945, "start": 2, "stop": 54 } ], "seq_stats": { "molecular_weight": 5613.2896, "isoelectric_point": 4.686511421203612 }, "id": "IGIGPGHMMC_1371", "locus": "GCA000148625_01361" }, { "type": "cds", "sequence": "contig_1", "start": 782143, "stop": 782277, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGGTACTATCTAGACAACAAGATACATACATGTTGCAATTGCAACGTCAAGGACGTATGCTTACATTTCCACCTAACTTAGGTGAAGAAGCATTACAAATTGCAACAGCAATGGCTATGGAAAAGAAAGACTGA", "aa": "MVLSRQQDTYMLQLQRQGRMLTFPPNLGEEALQIATAMAMEKKD", "aa_hexdigest": "b6971b78d97cddeeb60895f45add9df0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 44, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 8.313813328425995e-07, "score": 24.226940155029297, "start": 1, "stop": 44 } ], "seq_stats": { "molecular_weight": 5083.906400000002, "isoelectric_point": 6.0970277786254865 }, "id": "IGIGPGHMMC_1372", "locus": "GCA000148625_01362" }, { "type": "cds", "sequence": "contig_1", "start": 782278, "stop": 782427, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAGTACAAATATGTCGATGTTAATAAAATTATGGACGATCCAAATAAGATTATTAGACATTTAGACATAGACGGTAAAGTAATTGAAAAAAATTACAAAGCACCACTAAGTAATGCTGAAGTTTTAGAAGCTTACAAATGA", "aa": "MKTKYKYVDVNKIMDDPNKIIRHLDIDGKVIEKNYKAPLSNAEVLEAYK", "aa_hexdigest": "ccdf004ab974bcd80bdb658e793756f4", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5753.6466, "isoelectric_point": 8.92650203704834 }, "id": "IGIGPGHMMC_1373", "locus": "GCA000148625_01363" }, { "type": "cds", "sequence": "contig_1", "start": 782689, "stop": 783327, "strand": "+", "frame": 1, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3", "UniRef:UniRef90_C4XFF3" ], "nt": "ATGAAAAATATTGATTATAAAAGAATTTTTAATAAAGGTATCCCCACAAATATTATTAACTTGTTGACTGAAATATATGAATATAAAGGAAGACAAAACATTTATATCAATAACAAGAATTTAGAAATCCAAAAGCTTAAAAAACAAGCTTTAATTGACAGTGCAGTTTATTCAAATAAAATAGAAAACATCAATATAAGCAAAAAAAGAATTAAAGATATTTTGCTTGAAAATGATGAGTTGCAAAATGAAAATGAAGAAGATATTAAAGGTTATTTTGAATTATTAAATTCTCTATATAATCAAGAAACATTTGAATTATTAAACACTAATTTGATTCTTGATCTTCATTATCAATTGTTTCAAAACTCAAATACCAAAATGAGTGGGCGTTTTAAGTTAAATGATAATTTTATTTTAGAAACAACCTTAGAAGGTACTCAAGAAATAAAATTTATTCCTGTCAAAGCATTTTTTATTAGTGAATATATAAATAATTTATGTAATAAATATAATGAAGAATATAAAAGTAAGAAGATTAATTCATTGCTTCTAACAACAGCTTTTGTTTTGGATTTTATTTGCATTTCGCCTTTTAATCACGGCAATAGCAGAATGTCTAGAATTCTTTTAAATTGA", "aa": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLN", "aa_hexdigest": "6769c60eb1042ae27a758429ca2b00fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 782689, "stop": 783630, "observations": { "mutation": [ 783325, 783436 ], "stop": [ 783325, 783436 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF3", "query_cov": 1.0, "subject_cov": 0.6074498567335244, "identity": 1.0, "score": 414.0, "evalue": 5.27e-144, "gene_start": 1, "gene_end": 212, "reference_start": 1, "reference_end": 212, "reference_sequence": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLNWLLSVGDIKVVQYISLEKIIFENLEKYQNVQQKSSYKWHDNLNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH" }, "paralog": false, "identity": 0.9936102236421726, "score": 610.0, "evalue": 1.06e-223, "description": "Internal stop codon at 783325, 783436. Nonsense mutation around 783325, 783436.", "psc": { "uniref90_id": "UniRef90_C4XFF3", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF3", "UniRef:UniRef50_C4XFF3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3" ], "product": "Fido domain-containing protein" } }, "id": "IGIGPGHMMC_1374", "locus": "GCA000148625_01364" }, { "type": "cds", "sequence": "contig_1", "start": 783448, "stop": 783738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAACTATTATTACTTTATAGAGTTTATTTTAAAAATTGTTTTACAAGCTTATAAAGAATTTGAAAGCCGTTTTACAAGTTTAGAAAATAAAAAAATTGATGTAGCGGACAACATTTTAAAACTGATAGCTCAATATCAAAAAGTAAGTAAAAATGAGCTAATTAATTTACTACCTGAAAGCAGTAAAAGAACAATTGAAAGAAAACTAAATGTCTTATTAAAAGCTAAAAAAATACTTAAAATTAATCAAGGCAAAAATACTAAATACACTATAAAACAGCATTAA", "aa": "MNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH", "aa_hexdigest": "d10838ab1d375a70a886bae245ca4225", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11452.370099999986, "isoelectric_point": 9.838729667663571 }, "id": "IGIGPGHMMC_1375", "locus": "GCA000148625_01365" }, { "type": "cds", "sequence": "contig_1", "start": 783829, "stop": 784029, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_041593951.1", "SO:0001217", "UniParc:UPI0002DC7513", "UniRef:UniRef100_UPI0002DC7513", "UniRef:UniRef50_UPI0002DC7513", "UniRef:UniRef90_UPI0002DC7513" ], "nt": "ATGAAAAGAAAAACAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAAATTATAG", "aa": "MKRKTINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKKL", "aa_hexdigest": "0b236115fece37c4f326a4f60a574bae", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002DC7513", "ncbi_nrp_id": "WP_041593951.1", "uniref100_id": "UniRef100_UPI0002DC7513", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DC7513", "RefSeq:WP_041593951.1", "UniRef:UniRef100_UPI0002DC7513" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002DC7513", "uniref90_id": "UniRef90_UPI0002DC7513" }, "psc": { "uniref90_id": "UniRef90_UPI0002DC7513", "uniref50_id": "UniRef50_UPI0002DC7513" }, "pscc": { "uniref50_id": "UniRef50_UPI0002DC7513", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002DC7513" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7802.9757, "isoelectric_point": 7.9439393997192385 }, "id": "IGIGPGHMMC_1376", "locus": "GCA000148625_01366" }, { "type": "cds", "sequence": "contig_1", "start": 784073, "stop": 784258, "strand": "+", "frame": 2, "gene": null, "product": "transposase", "db_xrefs": [ "RefSeq:WP_258408847.1", "SO:0001217", "UniParc:UPI00030AD1D9", "UniRef:UniRef100_UPI00030AD1D9", "UniRef:UniRef50_UPI00030AFE14", "UniRef:UniRef90_UPI00030AFE14" ], "nt": "ATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAAAGATGTAAGTGATTTTTGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAA", "aa": "MEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTKDVSDFCLANMALIILQHK", "aa_hexdigest": "1747b1cba835577f685e335f8ca71dc5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00030AD1D9", "ncbi_nrp_id": "WP_258408847.1", "uniref100_id": "UniRef100_UPI00030AD1D9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00030AD1D9", "RefSeq:WP_258408847.1", "UniRef:UniRef100_UPI00030AD1D9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00030AD1D9", "uniref90_id": "UniRef90_UPI00030AFE14" }, "psc": { "uniref90_id": "UniRef90_UPI00030AFE14", "product": "transposase", "uniref50_id": "UniRef50_UPI00030AFE14" }, "pscc": { "uniref50_id": "UniRef50_UPI00030AFE14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030AFE14" ], "product": "transposase" }, "genes": [], "id": "IGIGPGHMMC_1377", "locus": "GCA000148625_01367" }, { "type": "cds", "sequence": "contig_1", "start": 784480, "stop": 784746, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "b1d2697471a467a119612aa745a6f757", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 7.451184768013668e-09, "score": 32.01397705078125, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10129.612099999993, "isoelectric_point": 8.806848716735843 }, "id": "IGIGPGHMMC_1378", "locus": "GCA000148625_01368" }, { "type": "cds", "sequence": "contig_1", "start": 784807, "stop": 784974, "strand": "+", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTATTCTCCTGAAATAAGAACGCTTATTTATACTACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAACATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSPEIRTLIYTTNPIENLNSVFRKYTKTKRVFPSDDSLLKILFLTSQQIIKK", "aa_hexdigest": "698e33389eb79873bcb6c9a41c2333aa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 67.0, "evalue": 1.21e-13, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "IGIGPGHMMC_1379", "locus": "GCA000148625_01369" }, { "type": "cds", "sequence": "contig_1", "start": 785358, "stop": 785744, "strand": "-", "frame": 3, "gene": null, "product": "DUF402 domain-containing protein", "db_xrefs": [ "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_D3VRN0", "UniRef:UniRef90_C4XF90" ], "nt": "ATGCCTAAAAATAGTATGTATAATGCCTTGATTTTATTAAAACCAAAAAGTAATTATGTCTATGTAAATTTAGCTTCCAAACCATATTATGAAGATAATACAATCAAATTTGTTGATTTTGATTTAGATGTTAAAAGCTATCCTAAAAAAGATTTTACAATTGTAGATAAAGAAGAATTTCGTTACAATTCTAGACGCCTCAAATATCCTAAAAAACTGATAAATATGATTTACGATAATTTAGAGGAAATAGTTAATAAACATAATAATGGTGAGTATTTCTTTAATCCAGATGTAATTGATTATTACATCAATCAAGCTAAAAAAGACAAATCAATTTATCGTGATTTTAGAGTTAAAAAAGCAAGTAGTTATAGTTTTAAATGA", "aa": "MPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFK", "aa_hexdigest": "44b38b8bc1d95021df16e688c3f81a6c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 785337, "stop": 786035, "observations": { "mutation": [ 785360, 785750, 785774, 785864, 785999 ], "stop": [ 785360, 785750, 785774, 785864, 785999 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF90", "query_cov": 1.0, "subject_cov": 0.5541125541125541, "identity": 1.0, "score": 259.0, "evalue": 3.2e-86, "gene_start": 1, "gene_end": 128, "reference_start": 98, "reference_end": 225, "reference_sequence": "MKNMEYILKGGKWMSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQWNGVKVLRNTPQHYVLFMYKTKVAETDKKGWVYREYVLWFMPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFKWRNKNK" }, "paralog": false, "identity": 0.9698275862068966, "score": 446.0, "evalue": 2.76e-162, "description": "Internal stop codon at 785360, 785750, 785774, 785864, 785999. Nonsense mutation around 785360, 785750, 785774, 785864, 785999.", "psc": { "uniref90_id": "UniRef90_C4XF90", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_D3VRN0", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF90", "UniRef:UniRef50_D3VRN0", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRN0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRN0" ], "product": "DUF402 domain-containing protein" } }, "id": "IGIGPGHMMC_1380", "locus": "GCA000148625_01370" }, { "type": "cds", "sequence": "contig_1", "start": 785862, "stop": 785996, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAGTTAATAAGGATAATCTTCTTATTAAAGAAGAAAATTTAAAACCTAAAAAAATAGTTTTTCCCTTAATTGGATCAATTATTAATGTCCAAGCTTTTAAATTTAATGGTGAATTATACCGCCAATGA", "aa": "MSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQ", "aa_hexdigest": "a192cefd794e14205ccb8513098c5cb7", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5104.018300000002, "isoelectric_point": 9.601614952087402 }, "id": "IGIGPGHMMC_1381", "locus": "GCA000148625_01371" }, { "type": "cds", "sequence": "contig_1", "start": 785983, "stop": 786177, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGATAATGAATTAATCAAAGAATATGAAAAAGATCCATTGCCAGATAGATATTTAACTAGTAGTGATGCACCTAGTGAATTAGATTCAACGAATTTATATAAAATAGAAGCTAGCTTTATAAGCAAAAAACAATCGAATAAAAAATATGGAATATATTTTAAAAGGAGGGAAATGAATGTCTTTAGTTAA", "aa": "MQDNELIKEYEKDPLPDRYLTSSDAPSELDSTNLYKIEASFISKKQSNKKYGIYFKRREMNVFS", "aa_hexdigest": "b4661cc32c3db0683a01004b7c4d0674", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7601.448299999999, "isoelectric_point": 6.205590248107909 }, "id": "IGIGPGHMMC_1382", "locus": "GCA000148625_01372" }, { "type": "cds", "sequence": "contig_1", "start": 786202, "stop": 786669, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAAGCTCAATTAGAAGCTGATCGAAACTTTTATAGTAACAATATTTATAAAAGTGAAAATCAACAACTATTTTACAAATTGTTTCTTGAAACAATGGGTTATGGTAAAACTATAGCTCAAGTTAAAAGCAAAATAGATGTTAAAAAATTAATTGCTGGAGACACTGATAGTTATTCAGTTGAAATTGATAAAAATAATATTTTTCAAACAAGAATTAGTGGTTATTTAGATACCAAAAATATCAAAGGATTTTATTTCACAAATAATAAAGATGAAAAAAAGTTTGTTAAAATAAATTATTTAAATGCATTTAAATTTGAGGGAAAAGATAATTTATTAGATACTACTTTTACCTTATCTCCAAATATGGTTTTAAGTGATTTAATTTCAAAACAAGCAGCTTATACAAGTGATTATTTTGATTATAGAAATATAAAAGAAAATTCACCTATTGAATTTTGA", "aa": "MHKAQLEADRNFYSNNIYKSENQQLFYKLFLETMGYGKTIAQVKSKIDVKKLIAGDTDSYSVEIDKNNIFQTRISGYLDTKNIKGFYFTNNKDEKKFVKINYLNAFKFEGKDNLLDTTFTLSPNMVLSDLISKQAAYTSDYFDYRNIKENSPIEF", "aa_hexdigest": "608021e83e1ff401a586438a1f09f199", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18122.249399999986, "isoelectric_point": 8.550909233093265 }, "id": "IGIGPGHMMC_1383", "locus": "GCA000148625_01373" }, { "type": "cds", "sequence": "contig_1", "start": 786856, "stop": 786996, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCTATTAAAGATGAAAATGACAAAGTAACTTTTAGAGAAACTAAAGATTTCGATTCAAAATATTTAATAGCCGATTATTCAATAAATAGTTTGCCTTATTATTATTCTCTAGTTGAACTAGTGCCAAGAGAATGA", "aa": "MKSIKDENDKVTFRETKDFDSKYLIADYSINSLPYYYSLVELVPRE", "aa_hexdigest": "574ec02ac4110de8a8b850d17618d522", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.1548, "isoelectric_point": 4.920460700988768 }, "id": "IGIGPGHMMC_1384", "locus": "GCA000148625_01374" }, { "type": "cds", "sequence": "contig_1", "start": 787285, "stop": 787491, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGGACCGGAAATAAAATTTTTAGATTCTATAAACGTTAATGACTTTAGTATTATCACTAAAGAATCTGCAGGTATTTATTTGCCAACAATCCAAAGAATTTATTTAAATGGTGCGGCACTAAGTGAAAAAGGAGCAAAATTAAGTGTCAAAGTAAAACAAATACTTTATTCACTTTTTCATGAATACATGCACCATTGA", "aa": "MTYGPEIKFLDSINVNDFSIITKESAGIYLPTIQRIYLNGAALSEKGAKLSVKVKQILYSLFHEYMHH", "aa_hexdigest": "356b010b2c3ef79291ab8d8f2b341330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7746.9088, "isoelectric_point": 8.02297763824463 }, "id": "IGIGPGHMMC_1385", "locus": "GCA000148625_01375" }, { "type": "cds", "sequence": "contig_1", "start": 787660, "stop": 787971, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTTTTGTTTCATTGTTTGTTGCTGTTTATTTACTTTTAAATAATAAAAATGAAACAATAAATAAAAATAATAATTTAGTTAAAATAAAAATACAAAGTAAAGAAGATAAAATAGGACTTATAAATCAAGATAAATATATTGAACAATATGCAAAAAGTCCTTCTTCATTAAATAATTTTGACAATGTTACAAATGATTTAAAAAATCAATTTATTGATTTAAAATTTAATGATTATATTTTGGCTAATAACACTGTTTTAAAGTATGATACGAAAAAACATAGCTATAAATATGATAAGAGAAATTGA", "aa": "MCFVSLFVAVYLLLNNKNETINKNNNLVKIKIQSKEDKIGLINQDKYIEQYAKSPSSLNNFDNVTNDLKNQFIDLKFNDYILANNTVLKYDTKKHSYKYDKRN", "aa_hexdigest": "b49c3b105b6b078a6e81cf5fca7a2304", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12145.707299999984, "isoelectric_point": 9.256064414978024 }, "id": "IGIGPGHMMC_1386", "locus": "GCA000148625_01376" }, { "type": "cds", "sequence": "contig_1", "start": 788286, "stop": 788501, "strand": "+", "frame": 3, "gene": null, "product": "MAG6790 family protein", "db_xrefs": [ "RefSeq:WP_013354908.1", "SO:0001217", "UniParc:UPI0001E32FAB", "UniRef:UniRef100_C4XF88", "UniRef:UniRef50_C4XF88", "UniRef:UniRef90_C4XF88" ], "nt": "ATGTATAAATTTAAAGCAAAATTAGTTTCAACTCAAGAAGTTGTTGCACAAGCAAACTCACTTGAAGAAATCGAAGGATTAATCCTTGGCTTCCGTCGTAAACAAAAATATGACGAACACACAAGAGCCAACGAAAAAATCCAAATTATACATGTTGAAAGGGATTCTTTAAAAGGTAAACATAAATCAAAAGAAGAAATTCTTAAAGTTGTTTAA", "aa": "MYKFKAKLVSTQEVVAQANSLEEIEGLILGFRRKQKYDEHTRANEKIQIIHVERDSLKGKHKSKEEILKVV", "aa_hexdigest": "3aa7ea5bd06dae26f05da1144afc132b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FAB", "ncbi_nrp_id": "WP_013354908.1", "uniref100_id": "UniRef100_C4XF88", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FAB", "RefSeq:WP_013354908.1", "UniRef:UniRef100_C4XF88" ] }, "ips": { "uniref100_id": "UniRef100_C4XF88", "uniref90_id": "UniRef90_C4XF88" }, "psc": { "uniref90_id": "UniRef90_C4XF88", "product": "MAG6790 family protein", "uniref50_id": "UniRef50_C4XF88" }, "pscc": { "uniref50_id": "UniRef50_C4XF88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF88" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_1387", "locus": "GCA000148625_01377" }, { "type": "cds", "sequence": "contig_1", "start": 788674, "stop": 788913, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAGATTTTTATTAGGTGCTTTAGCTCCAGTTGTTGCCTTAAGCCCTATTGCTATTGCAGTTAGTTGTGGAAAACAACCAACCATTGAAGAACAATTCGCTAAATTAAATAATGAAGGAAAATTAGCCTTTATTAAAGCCCTTGACTACAAAGGAATTATGTCAATGGACGAAAAAGAAAAATTAATTTCTAAATTAAATAAAGATGCTGGTCAATTTGGATCAATTGTTTGA", "aa": "MKKRFLLGALAPVVALSPIAIAVSCGKQPTIEEQFAKLNNEGKLAFIKALDYKGIMSMDEKEKLISKLNKDAGQFGSIV", "aa_hexdigest": "c5be69532592b9ffd7bf02755a0c8785", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8567.113699999996, "isoelectric_point": 9.374621772766112 }, "id": "IGIGPGHMMC_1388", "locus": "GCA000148625_01378" }, { "type": "cds", "sequence": "contig_1", "start": 788989, "stop": 789363, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGTGCTGCAACCGATGGTTTTGAATATGGTCAAAACTTTAATACAACTAAACAAGTAAATAATGCTGCAACAGATAAATACATTCCAGTTATCTTTATGGACATTGATGAAACTGTTTTACAAAATGATTACACAGAAACATTTGGTATGTTAAATGGTGGTTACAATGGAGACATGAAAGAAAAGAACGACCTCAAAGGAAATAGATTTGCTGTGCCAGGTGCTATTGATTTCATTAACTATGTACAATCAAAAGGTGCTTTAGTTGTTTATAACTCAGATATGAACCAATCTACAGCAGTTAGAGAAGCTGTTAAAAAGAATTTGATAAACTTAGGTGTTAAATATGTTTCAGACTTCCAATTCTGA", "aa": "MKRAATDGFEYGQNFNTTKQVNNAATDKYIPVIFMDIDETVLQNDYTETFGMLNGGYNGDMKEKNDLKGNRFAVPGAIDFINYVQSKGALVVYNSDMNQSTAVREAVKKNLINLGVKYVSDFQF", "aa_hexdigest": "9d004f586c3847be07decdf6c0986a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13916.450899999985, "isoelectric_point": 5.032604026794433 }, "id": "IGIGPGHMMC_1389", "locus": "GCA000148625_01379" }, { "type": "cds", "sequence": "contig_1", "start": 789364, "stop": 789504, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGTTCAATGCCTTATGTAGCAATGGATGGAACTAAAGCTATTACAGACGAAATGACAAAAGATATGTCTAAAAACGATCTTAAAAGTCTAGCTGGAAGAATGAAATTTACAAAAACTTTTGAAACTACACCATGA", "aa": "MRGSMPYVAMDGTKAITDEMTKDMSKNDLKSLAGRMKFTKTFETTP", "aa_hexdigest": "e445eddd0727388dc670c731e6c77ef8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5162.9785, "isoelectric_point": 9.223185539245605 }, "id": "IGIGPGHMMC_1390", "locus": "GCA000148625_01380" }, { "type": "cds", "sequence": "contig_1", "start": 789655, "stop": 789849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAGGCGACAACTTTAATGACTTCTTTGATAGAAACTCAAAAGGTAAAGACAATGACAAAAGAACTCAACAATACGTTAAAAACACAGACATGAGAAAATTATTCACAGATATTAATGGTGCTCCAGGATTAAGATTTGTTAAAAAAACAGATGGTAAATATGGAGAATTTCAAAAAACAACATACTGA", "aa": "MRIGDNFNDFFDRNSKGKDNDKRTQQYVKNTDMRKLFTDINGAPGLRFVKKTDGKYGEFQKTTY", "aa_hexdigest": "b0924d8bc7d2ef17aacf8f4ff5c80bb2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7561.359300000001, "isoelectric_point": 9.69528751373291 }, "id": "IGIGPGHMMC_1391", "locus": "GCA000148625_01381" }, { "type": "tRNA", "sequence": "contig_1", "start": 790117, "stop": 790192, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 78.2, "nt": "GCGTCCTTAGCTCAGGCGGTAGAGCAAATGGCTTTTAACCATTGGGTCGGAGGTTCGAGTCCTCTAGGACGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 790158, 790160 ], "id": "IGIGPGHMMC_21", "locus": "GCA000148625_01382" }, { "type": "tRNA", "sequence": "contig_1", "start": 790221, "stop": 790307, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 67.7, "nt": "GCCCAGGTGGCGGAATAGGTAGACGCAAGGGACTTAAAATCCCTCGAACGTTAGTTCGTGCTGGTTCAAGTCCAGTTCTGGGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 790271, 790273 ], "id": "IGIGPGHMMC_20", "locus": "GCA000148625_01383" }, { "type": "cds", "sequence": "contig_1", "start": 790468, "stop": 790638, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAATAAAATTTTTAATAGTTTATTATTAACTCCATTAATTATAACTCCTATAACATCTTGTGGTAGTAAGCCAGAAGAAAAAAAGTCATTACCATTACCCAGATCTTGCACAGATTATGACAAAAAAGAATTGAATAAAATAGTTGAAAAAAATACAAAATGA", "aa": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSLPLPRSCTDYDKKELNKIVEKNTK", "aa_hexdigest": "dee17be898d2f86640d67b76b6f000a5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6375.521800000002, "isoelectric_point": 9.597360038757323 }, "id": "IGIGPGHMMC_1392", "locus": "GCA000148625_01384" }, { "type": "cds", "sequence": "contig_1", "start": 791083, "stop": 791655, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FAD", "UniRef:UniRef90_UPI0001E32FAD" ], "nt": "ATGTTAAGACTTTCTTTATTAAAAAACTGTAATATTGATTTTGACTACAGTGTCAACGATATATTTATCAAACGAATTAATATAAAATTAACAGAATTCGACAATGTTCTAATTAACTCCTTTAGTGACCCAAAATATTCTAGTTATTACACAAATGAAATTTATTTCTTTTTAAATAATTTTAATAGTGATAAACAAAATATCATTGTTGATAAAAATAATTTTGATTATTATAGAGCAAATCAATTTCAAAATGTAAAAATAAACACTTTAACATTAAACAATTTTGAACCTGATTCGGTTGAAAATGCAAATGTTATAGCAACCGATATAGATGTTAATACTATAGAATTTAGTGATGAATTTTCAAATAATATTGATATAGATGAAACATTTAAAAATATAAATTTCAAAGATATAAAAGGTGTTCAAAATGTGTTAAAAAATTTGAATTGTGTAAGTTCAAAAGATAAATACATTTTATTTGAAAAATTAACTAAAATAAATAAGTTGCCCGCTTCATTTAAAATTAATTTAAAAAAGATACATTATACAATAATGATTTATCAGTAG", "aa": "MLRLSLLKNCNIDFDYSVNDIFIKRINIKLTEFDNVLINSFSDPKYSSYYTNEIYFFLNNFNSDKQNIIVDKNNFDYYRANQFQNVKINTLTLNNFEPDSVENANVIATDIDVNTIEFSDEFSNNIDIDETFKNINFKDIKGVQNVLKNLNCVSSKDKYILFEKLTKINKLPASFKINLKKIHYTIMIYQ", "aa_hexdigest": "fcfd604c0d6b5301ae69035c19bfb815", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 790783, "stop": 791655, "observations": { "mutation": [ 790861 ], "stop": [ 790861 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FAD", "query_cov": 1.0, "subject_cov": 0.4810126582278481, "identity": 1.0, "score": 367.0, "evalue": 1.8e-125, "gene_start": 1, "gene_end": 190, "reference_start": 206, "reference_end": 395, "reference_sequence": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSLPLPRSCTDYDKKELNKIVEKNTKWIENSKTHELVKYIDYDFFYDVVKKWYCDDNNNLKSDIFYVPAINKISVLKEKNNIDLVSSYIFSTKKDLQEELDWINNNKKKAIQKYSKRNETLSDEENLKKVKKEIENEIKIRENKRDIFSFKKFVISSNFFTLETNDLQDHKFKTSMLRLSLLKNCNIDFDYSVNDIFIKRINIKLTEFDNVLINSFSDPKYSSYYTNEIYFFLNNFNSDKQNIIVDKNNFDYYRANQFQNVKINTLTLNNFEPDSVENANVIATDIDVNTIEFSDEFSNNIDIDETFKNINFKDIKGVQNVLKNLNCVSSKDKYILFEKLTKINKLPASFKINLKKIHYTIMIYQ" }, "paralog": false, "identity": 0.996551724137931, "score": 573.0, "evalue": 4.94e-209, "description": "Internal stop codon at 790861. Nonsense mutation around 790861.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FAD", "uniref50_id": "UniRef50_UPI0001E32FAD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FAD", "UniRef:UniRef50_UPI0001E32FAD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FAD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FAD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 22418.157700000018, "isoelectric_point": 5.478960609436036 }, "id": "IGIGPGHMMC_1393", "locus": "GCA000148625_01385" }, { "type": "cds", "sequence": "contig_1", "start": 792045, "stop": 792269, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAAAACATTAAATCCTATTTACAATATTACAAAAAATATGGACTTGTTTAGAGCTGAAAATTATATGGACACTTTAGATATTAATACATTTAATTTTGAATTAACAGAGAAAAGTTTGATGGGATTTAAAACAATCAGATGTTTAAAAGATGATTTTGAAATTTTCAAAGAAAAAAATATGGAAAAAACAACAAATAATAATGGAGAAGAAGTTTGA", "aa": "MESKTLNPIYNITKNMDLFRAENYMDTLDINTFNFELTEKSLMGFKTIRCLKDDFEIFKEKNMEKTTNNNGEEV", "aa_hexdigest": "8f8106aa575368676c84cbbdeeb297ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8811.912299999993, "isoelectric_point": 4.732835197448731 }, "id": "IGIGPGHMMC_1394", "locus": "GCA000148625_01386" }, { "type": "cds", "sequence": "contig_1", "start": 792324, "stop": 792449, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAATAAATTAAGTTTTATTTTTGAATGCAATAATGGAACATACACATTAATTCCTGATAAAGTGCCTTACTTTTACGATGGAAATTATTTAACATATAATATAACAAAAATAAAATAG", "aa": "MEYNKLSFIFECNNGTYTLIPDKVPYFYDGNYLTYNITKIK", "aa_hexdigest": "cfe6e13862270492afbf66182e259e5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.5799000000015, "isoelectric_point": 5.967207527160644 }, "id": "IGIGPGHMMC_1395", "locus": "GCA000148625_01387" }, { "type": "cds", "sequence": "contig_1", "start": 792498, "stop": 792722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAAATATTGATCAAATTAAAGGCAAAACTAAAGAAAAATTATTAGATGAAAAAGATCAAGCTTTTTTATGTAAAGCATTAGCAAAATTAAATTTAAATGTTGATATGAATAAAAACATTTCTGATTACCTATTGAACTTAAACTTAGAAGATGGCTTAGATGCAATGCGTTTTTATGGTTTAAATAAATGCGCAAATTTATTTGAAGAATTGAAATAA", "aa": "MYQNIDQIKGKTKEKLLDEKDQAFLCKALAKLNLNVDMNKNISDYLLNLNLEDGLDAMRFYGLNKCANLFEELK", "aa_hexdigest": "37366898112e2908f1ef2962eab44d26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8586.866399999995, "isoelectric_point": 5.294802284240721 }, "id": "IGIGPGHMMC_1396", "locus": "GCA000148625_01388" }, { "type": "cds", "sequence": "contig_1", "start": 793053, "stop": 793382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02739.22" ], "nt": "ATGAATAAAGATAACTTTTTATTAATAGATGGGAATTATTTATTATTTTCTTCTTTCTATGCTTCATATAATCCGGATAGATTAGAAAATATTATGCGTTCACCACAAGGTTTAACAACTAATGGTGTTCATGTTTTTCTTATGACTTTAAGTAAACTAATAGCATACTTTAGACCTAAATACTTATTTATTGCTTTTGATGCTCATGGAGAAACTAAAAGACATAAAATATTCAAAGATTATAAAGCAGGTAGAACTCAAGCACCAGAAATAATTTTTGAACAATTTAGTTTAATTAAAGACATTTTGACAAAATTAAATATCAAATGA", "aa": "MNKDNFLLIDGNYLLFSSFYASYNPDRLENIMRSPQGLTTNGVHVFLMTLSKLIAYFRPKYLFIAFDAHGETKRHKIFKDYKAGRTQAPEIIFEQFSLIKDILTKLNIK", "aa_hexdigest": "140464ef249ec988632a6de8b9bb7b40", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02739.22", "name": "5_3_exonuc_N", "length": 104, "aa_cov": 0.9541284403669725, "hmm_cov": 0.9326923076923077, "evalue": 8.520663961108346e-27, "score": 89.88484954833984, "start": 6, "stop": 109 } ], "seq_stats": { "molecular_weight": 12756.69479999998, "isoelectric_point": 9.521029472351074 }, "id": "IGIGPGHMMC_1397", "locus": "GCA000148625_01389" }, { "type": "cds", "sequence": "contig_1", "start": 793397, "stop": 795178, "strand": "-", "frame": 1, "gene": null, "product": "DNA-directed DNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4", "UniRef:UniRef50_UPI00244ECC9B", "UniRef:UniRef90_C4XF83" ], "nt": "ATGGCAATTAGAGATATGGGCAGAGTTATAGGAATTAGCTTAATTGACATCAACCGCATCAGTAAAACTATTAAATCAGATAACTTAACTGATGCTTATAAAAATGATATTGACTTTAAAATTGAAATGGATAATTATCCTGAGTTATATAAACATGCACTTAAAATCGAAGGCTTACCTCGTCAACAAAGTTTCCATGCAGCAGGTTTTATTATTGCTAAGGACAAATTATTTAATTATGTCCCTGTTTGTTTAAGCAATTTAGACAATTACCAACAAGTACAAGTACCAATGAATTACATAGAGAGTTATGGACTTTTAAAAATTGACTTGCTAGGTTTAAGAACTTTAACTGAAATTCAACTAATTGAAAAAAGTTTAGAAGAAAACCAAAAATTTGATTATTTAGTAAAAAGCGATCCTCAAATTTTAAATGATCCTAAAACTTTAACAATGCTCAACTTAGGTTTTACTGAAGGAATATTTCAATTAGAATCTCCGGGCATGAAGAAGACAATTCAAAAAGTTGTCTTGAATTCTTTTGATGATTTATATGCTATTATTTCATTATTTAGACCTGGACCTATGGACTTTATCAATGACTATGCAACTAATAAGAAAAATCAAAAATTAATTAAAAAAATACATCCTGATTATGACAAAGTTGTTGCCAACACATATGGCATAATAGTTTATCAAGAACAAATTATGCAAATAGCTCAAAGTATTGGCGGAATGACTTTTGCTCAAGCTGATTTATTGCGTAAAGCAATTAGCAAAAAAAGTGAAGTTGATATCAAAAAATATAGAAGCATATTTTTTGAAAATGCCATTAAAAAAGGCATAAACGAAAAAATTATTAATGCTATTTATAACAATATTGAAAAATTTGGACTATACGGTTTTAATAAATCTCATGCTGTTAGTTATGCATATTTAACTATGAAAATGTCATACTATAAAGCTCGTTATGCTAACTTATTTTATAGCTCTTTAATTACAAATGCAGCTGGAGCACAAGCTACAATTAATAAATATGTAGCTGAAATTAAGAAACTTAATTTTCCAGTTTATTCGCCAAATATTTTGCATTCAAGTAATAAATGTATAGTAACAAACGATGGCATTTATTTACCTTTTGAAATGATTAAAGGTTTTGGAGCTGAAGGTGTTAATAAAATTGTGGCTGATTTAAATGAAAATGGACCATTTCAAAAGAATTTAATTGATACAATGCTTAGACTTCGTTTTGCAGGTATTAAAGATGCTGCTATGGAAATATTAATTAAAGCCAATGCTTTTAGAGACTTTGGACACATGAAATATATATTATCTTGTGATAATCAAATTAAAGACATTTTTAAAGTTTTATCTTCACATTCAACTTATAAAGAAGCATTAAAAGATATCAAAAAAATGGGTTACTATGATATAGTTTTTGATCAAGAAAATGAACGTGACATAAATTACGAAATGGACTATGAAATAGCTCTTTTAGGTGAATTATACAATGCTTTTAGTACTTCAATTTATGAAGCAAAATACAAAAATAAATTAAATACAATAACCTCTTCAATTCCTGTTTCTGTTGCTGCTGAAGTAGCTGATTTAAGAAGACCAAGAGGTAAAGAATTTAGTGTTTTAGAATTAAGAGATAGTACAGCTAAAATTACTTTCTTTGTTAACAACAAAGTATTGGCAAGTTCACTTCCTCTTAAAAAAGGACAAATAATTGAAGCTACAATTAGTTTCAGTCGTAACAAACATAATTTAATTAGTTGA", "aa": "MAIRDMGRVIGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKSDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIKKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLIS", "aa_hexdigest": "582199d7152a7bce435dbf822de9107a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00244ECC9B", "query_cov": 0.8768971332209107, "subject_cov": 0.9683426443202979, "identity": 0.5, "score": 483.0, "evalue": 2.33e-162, "valid": false, "uniref50_id": "UniRef50_UPI00244ECC9B" }, "pscc": { "uniref50_id": "UniRef50_UPI00244ECC9B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244ECC9B" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 793382, "stop": 795478, "observations": { "mutation": [ 793399, 795448 ], "stop": [ 793399, 795448 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF83", "query_cov": 1.0, "subject_cov": 0.6132368148914168, "identity": 0.995, "score": 1152.0, "evalue": 0.0, "gene_start": 1, "gene_end": 593, "reference_start": 370, "reference_end": 962, "reference_sequence": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGAFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKAIEISELWNPNLIVLDHPRYGYYAQTKDNKYSASKNYYINSSDASLPNSILIKENKLFEASDNETLNYLQKLGNLEETKHYANYFDEVNVDSEIIDRIYSIINECNVIFPKKALHLAPFQNNDEATNEKLFVSLLKAGAKRLASELKNYPDWNKRLEYEFNVIKKLGYINYFLIIQDLVNWAKSQKIAIGPGRGSAAGSLVSYLLNITEINPLRYNLLFERFLNPDRVSWPDIDIDIQDDRRFEVFEYLQKKYGVDKTGLISTFQTIGAKMAIRDMGRVMGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKNDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIQKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLISWKGIQ" }, "paralog": false, "identity": 0.9928366762177651, "score": 1362.0, "evalue": 0.0, "description": "Internal stop codon at 793399, 795448. Nonsense mutation around 793399, 795448.", "psc": { "uniref90_id": "UniRef90_C4XF83", "product": "DNA-directed DNA polymerase", "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF83", "UniRef:UniRef50_D3VPK4" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4" ], "product": "DNA-directed DNA polymerase" } }, "id": "IGIGPGHMMC_1398", "locus": "GCA000148625_01390" }, { "type": "cds", "sequence": "contig_1", "start": 795533, "stop": 795853, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAACTCTATATTAGTAAAAGAAAATAAACTTTTTGAAGCTAATGATAATGAAACACTAAATTACTTACAAAAATTAGGCAATTTAGAAGAAACTAAACACTATGCCAACTATTTTGATGAAGTTAATGTAGATAGCAAAATAATTGACCGTATTTATTCAATAATCAATGAATGTAATGTTATTTTTCCTAAAAAAGCCTTGCATTTAGCTCCTTTTCAAAATAATGATGAAGCTACCAACGAAAAACTTTTTGTTTCGCTTTTAAAAGCAGGCGCTAAAAGACTAACAAGTGAATTAAAAAATTATCCAGAGTGA", "aa": "MPNSILVKENKLFEANDNETLNYLQKLGNLEETKHYANYFDEVNVDSKIIDRIYSIINECNVIFPKKALHLAPFQNNDEATNEKLFVSLLKAGAKRLTSELKNYPE", "aa_hexdigest": "b583a991d35724a2681dd7c6855cb107", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12248.738099999984, "isoelectric_point": 5.416665077209472 }, "id": "IGIGPGHMMC_1399", "locus": "GCA000148625_01391" }, { "type": "cds", "sequence": "contig_1", "start": 795965, "stop": 796285, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATATGTCAATTTATACAATACAAGTGAATACACTTTTTTAGATTCTTTAATTAGGATAGATGATTTAGTTAGATTATCAAAAGAACACAATCTTCCTGCAGTAGTTTTAAGTGACCGAAACAATATGTTCGGTTTAGGTGTTTTTATGGAAGCATGCTTAAAATATGATATCAAAGGCATTATAGGAGTTGACTTAGATGTAGATAATTATCGCTTCATAGTCTTAGCAAAAAACTACCAAGGTTACCAAAAAATTAATCAACTAATTTATCAAAAAAGTATCAATAAAGATATTGAAATTAGCGAATTGTGA", "aa": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGVFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKDIEISEL", "aa_hexdigest": "0aca9f47c5e50c8a62eb3306ab7594df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12374.163999999988, "isoelectric_point": 5.747240638732911 }, "id": "IGIGPGHMMC_1400", "locus": "GCA000148625_01392" }, { "type": "cds", "sequence": "contig_1", "start": 796389, "stop": 796643, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82", "UniRef:UniRef90_C4XF82" ], "nt": "ATGGCAAAAAAGATGAGCAAAGGTGCCATTATTGGTATATCAGTAGGAGCTACAATTGTAGTAGCTGGTGCAGTAACTGCTGCTCTTGTATGTACATTGCATGGAAAAACTGTTTATAAAATTGAATACTCTAAGTATAAAAGAGCTAGAGAAAAAGGCAATATTGGAGACAAAATAGCTTTAGCTACTTTAGAAGGACTTATTGTAACAGGTGAAACAACTTTGAAAGAAATAAATGGAACAAACCAACAATGA", "aa": "MAKKMSKGAIIGISVGATIVVAGAVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQ", "aa_hexdigest": "7e2ec05ff1d9015eb9678bc7e5ee085f", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 796329, "stop": 796769, "observations": { "mutation": [ 796641 ], "stop": [ 796641 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF82", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 0.9640000000000001, "score": 151.0, "evalue": 1.97e-45, "gene_start": 1, "gene_end": 84, "reference_start": 21, "reference_end": 104, "reference_sequence": "MFLYLVVKIVKLLYTFRRCIMAKKMSKGAIIGISVGATILVASTVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQWLDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK" }, "paralog": false, "identity": 0.9657534246575342, "score": 272.0, "evalue": 1.1e-95, "description": "Internal stop codon at 796641. Nonsense mutation around 796641.", "psc": { "uniref90_id": "UniRef90_C4XF82", "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF82", "UniRef:UniRef50_C4XF82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8807.267099999995, "isoelectric_point": 9.673819541931152 }, "id": "IGIGPGHMMC_1401", "locus": "GCA000148625_01393" }, { "type": "cds", "sequence": "contig_1", "start": 796644, "stop": 796769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAGCTAAATTTGTAAAAGACACTAGAAAAGAAATAATAGGAAATAAATCAAAAGCTGATTTTCAAAAATTAATTGATGATTTTAAAAATTTTTTAAAAAGTTTAAAAGTAAAAAAATAA", "aa": "MDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK", "aa_hexdigest": "0157d037a33d4ab7c75ab6e9e8358ce0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4829.7037, "isoelectric_point": 9.919701957702635 }, "id": "IGIGPGHMMC_1402", "locus": "GCA000148625_01394" }, { "type": "cds", "sequence": "contig_1", "start": 796801, "stop": 797265, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGCAGCTTTTTATGATCGTAGTGAATTCTTAAATTATTCAATTAGAATGCTTGATATTTATGAAGGAACAAAACTCAAAAACATTTTTACAGCTTTGGAAAACAATGCAAAATTTGGTTTAAAATCACTTAACCTATTTTACGAAAAGGTCATTAAAAAAGTTGTTTTCGACAAAAAAATAGTTGATTTATCACAATCAATTAAAAACACATTATTAGGTCGTGAACTAATACTTAATGAGTATTTTTCATCAACATCACTTAAATCATTTGACAATCATGCAATGTTTTTAAAATTTAAAGACGCAAAAGAAATGAATAAATATTTAAAGAAAATTGAACCAACTAAAATTCATTTTGAAACAAGCAAAGTATTCTTAAATTATCTTTCTCCAAAGGTAAATACAAACAAAGTTGACATTCGTTTTGTTTTAACTAAATTAGTTAACAATTATGTCTAA", "aa": "MFAAFYDRSEFLNYSIRMLDIYEGTKLKNIFTALENNAKFGLKSLNLFYEKVIKKVVFDKKIVDLSQSIKNTLLGRELILNEYFSSTSLKSFDNHAMFLKFKDAKEMNKYLKKIEPTKIHFETSKVFLNYLSPKVNTNKVDIRFVLTKLVNNYV", "aa_hexdigest": "bf7090c90e3190d404d3e8e6769c7931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18152.047100000003, "isoelectric_point": 9.631077003479003 }, "id": "IGIGPGHMMC_1403", "locus": "GCA000148625_01395" }, { "type": "cds", "sequence": "contig_1", "start": 797357, "stop": 797596, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAATTGAATCACTAAGTGTTTCTTCATTATTTACAAATTGTAATAATTTGAAAATGCTTGATGACATTAGTCAGTATCTATTAGGTATATTTACACCACGATATTTTTCAACTAAACTAGTTGGTATTAATTTTGCATATTCAATTTCAACTTGGTTATTGTTAATTTGCCCCACAAAAAGATTTTCTTTGTTTATTATTGCTTTATATTCTTTAATTTTGAAAACATGA", "aa": "MKYKIESLSVSSLFTNCNNLKMLDDISQYLLGIFTPRYFSTKLVGINFAYSISTWLLLICPTKRFSLFIIALYSLILKT", "aa_hexdigest": "8b269059cf18d5face1a4ee872d2143d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9125.817399999989, "isoelectric_point": 9.469454765319824 }, "id": "IGIGPGHMMC_1404", "locus": "GCA000148625_01396" }, { "type": "cds", "sequence": "contig_1", "start": 797665, "stop": 797817, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAATTTAAAAGTTAATCTTAAATTAAAAGAATATAACGAATTAAAAAGATTAATATTTGATTTAGACACAAATATGAAAGAAACTATCCAATCAGTTACTAATAAAGAAAAGATTACATTATTAGATTCAACAGCTTTAATGTGA", "aa": "MLNNLKVNLKLKEYNELKRLIFDLDTNMKETIQSVTNKEKITLLDSTALM", "aa_hexdigest": "cfe4bc8845d516f8858587124dd4f538", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5869.8679, "isoelectric_point": 8.034904289245606 }, "id": "IGIGPGHMMC_1405", "locus": "GCA000148625_01397" }, { "type": "cds", "sequence": "contig_1", "start": 797983, "stop": 798138, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTATTAAGAAACTTAAACCATATTTTGAAAAATACAAAAGATCACAAGACAAAATTCATAATTTGATGTTATTAGATTGGTTGATAGCTCTTCTTTTAGTTTTATCATCTGTTATTATGGGAGTTTTACTTCATTATTTAAATAATTGA", "aa": "MLFIKKLKPYFEKYKRSQDKIHNLMLLDWLIALLLVLSSVIMGVLLHYLNN", "aa_hexdigest": "c7b85156445c61eadc330b4514ac14f8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6118.452799999997, "isoelectric_point": 9.698381996154787 }, "id": "IGIGPGHMMC_1406", "locus": "GCA000148625_01398" }, { "type": "cds", "sequence": "contig_1", "start": 798238, "stop": 798789, "strand": "-", "frame": 2, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "GO:0006412", "GO:0008135", "RefSeq:WP_013354916.1", "SO:0001217", "UniParc:UPI0001A8C2B1", "UniRef:UniRef100_C4XF80", "UniRef:UniRef50_Q98QZ1", "UniRef:UniRef90_C4XF80" ], "nt": "ATGGAATTAGATTTATATTTATTAGATTTTTTAGATGAGGCTGAAAAAGCAATTAATCATTATGCTTTTGAATTATCAAAAATTTCAACTGGACGTGCTAACCCTCAATTAATTAAGGGAGTTAGAATTAATTATTATGATACTTTGACACCTCTTGAAGAACTTGCTTCAATTACAGTACCAGAACCACAACAATTATTAATTAAACCTTTTGACGTTTCATGCAACCATGAAATTGCAAAAACAATTTTAAATGCTAACTTAGGTTTAAATCCTGTTGATGAAGGAAATCAAGTTAGAATAACTTTTCCTCCTCTTACAACTGAAAGACGTAAAGATATGGTTAAGAGTCTTGCAAAATTTACAGAAAATGCTAAAGTTGCAATTAGAAATGCAAGACAAAATGTTAACAAATCTATCAAGGCTGATGAAGAATTATCAGAAGATCAAGAAAAGAAATACTTAGATGAAATTCAAAAAGAAGTTGATAAAAAAATCGAACACATTAACAAAATGACAATCAACAAAGAAAAAGAATTAATGAGCATATAA", "aa": "MELDLYLLDFLDEAEKAINHYAFELSKISTGRANPQLIKGVRINYYDTLTPLEELASITVPEPQQLLIKPFDVSCNHEIAKTILNANLGLNPVDEGNQVRITFPPLTTERRKDMVKSLAKFTENAKVAIRNARQNVNKSIKADEELSEDQEKKYLDEIQKEVDKKIEHINKMTINKEKELMSI", "aa_hexdigest": "256d7dc815b02298fa3ae94b2c0cbff1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C2B1", "ncbi_nrp_id": "WP_013354916.1", "uniref100_id": "UniRef100_C4XF80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2B1", "RefSeq:WP_013354916.1", "UniRef:UniRef100_C4XF80" ] }, "ips": { "uniref100_id": "UniRef100_C4XF80", "uniref90_id": "UniRef90_C4XF80" }, "psc": { "uniref90_id": "UniRef90_C4XF80", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_Q98QZ1", "go_ids": [ "GO:0006412", "GO:0008135" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QZ1" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "IGIGPGHMMC_1407", "locus": "GCA000148625_01399" }, { "type": "cds", "sequence": "contig_1", "start": 798789, "stop": 799280, "strand": "-", "frame": 3, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGTTGGCAACTATCATGAATGGTCTAGCTCTTAAAAGTGGATTCGAAAAAGTTGGATTAAAAACTAGAATCCAATCATCAATTGCAATTGATCAAAAAGTTGCAGAAAATTATATTAATGAAAAGACTTTAAAATACCTTGAAGAAGGTGAAGTAGTTATTTTTGTTGGCGGTACAGGTCGTCCTTACTTTACAACAGATACAGCAGCAACATTATTTGCTTCAGAAATCAAAGCTGAAGTTATCTTAATGGGTAAAAATGGTGTTAGTGGAATCTATGATTCAGACCCTAAGAAAAATCCAAATGCCAAGAAATATGATGTTGTAACTTATGATGAAATTTTAGACAAAAAATTACAAGTCATGGACTTAACGGCCACAAGTATGGCTAGAGACAACAATATTAATTTAATTGTTTTCAACATTAAAGAAAAGAATGCTATTGTCAAAGCTCTTGAAGGTAAAATTGAACACACAGAGGTAATTAAATAA", "aa": "MLATIMNGLALKSGFEKVGLKTRIQSSIAIDQKVAENYINEKTLKYLEEGEVVIFVGGTGRPYFTTDTAATLFASEIKAEVILMGKNGVSGIYDSDPKKNPNAKKYDVVTYDEILDKKLQVMDLTATSMARDNNINLIVFNIKEKNAIVKALEGKIEHTEVIK", "aa_hexdigest": "04f05b835647ab07dc625ab87faab339", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 1.0, "subject_cov": 0.9588235294117647, "identity": 0.595, "score": 204.0, "evalue": 7.06e-65, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "IGIGPGHMMC_1408", "locus": "GCA000148625_01400" }, { "type": "cds", "sequence": "contig_1", "start": 799338, "stop": 799505, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATACAAACGTATATTAGTAAAACTTTCAGGTGAAGGTTTAGTAAATAAACAAAAAAGTTTAGCTATTGACTATGATTTAGTAGAAGACATTGCTAAACAGCTTAAAAAAATTATTGACAAAGGTGTTCAAGTTAGTGTTGTTATTGGTGGAGGTAACTTTTGA", "aa": "MKYKRILVKLSGEGLVNKQKSLAIDYDLVEDIAKQLKKIIDKGVQVSVVIGGGNF", "aa_hexdigest": "467ce7109c83ee61f555fab9336f2359", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.142000000002, "isoelectric_point": 9.573829078674315 }, "id": "IGIGPGHMMC_1409", "locus": "GCA000148625_01401" }, { "type": "cds", "sequence": "contig_1", "start": 800364, "stop": 800765, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAGAAAAGAAACATTTTTCGAACGTTTGACAAGAAAAAATGCCGAACACGAATATAAAAATCAACAAGCAATGCCTAAAAAGAAAAAGCTAAAAGGTATAATCCTTGGAACACTTTTAACATCTGCAATTGCAACAGGTATCATTGTTCCAGTTGTAATTAATTCAACAAAAGTAACATATGTAGATGCACTCAAAGATGAAAGTGCTGTTTTAACATTTACATTGCCAGGATCAAAAGAAAAACATTCTATTACAATCAAAGATTTAAAACATTCAAATAAAACTGAAGATGCTAAAGATCCAAGTAAATTGTTACAACAAATTAAACGTCTTGCGATGTTTTATCTTTATAATAAAGAACAAAAAGCCTCAGCTGAATATCAAAGATTATGA", "aa": "MAKRKETFFERLTRKNAEHEYKNQQAMPKKKKLKGIILGTLLTSAIATGIIVPVVINSTKVTYVDALKDESAVLTFTLPGSKEKHSITIKDLKHSNKTEDAKDPSKLLQQIKRLAMFYLYNKEQKASAEYQRL", "aa_hexdigest": "94d0b51a199c0719c8691502ecc17b50", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15181.57539999998, "isoelectric_point": 9.862969779968264 }, "id": "IGIGPGHMMC_1410", "locus": "GCA000148625_01402" }, { "type": "cds", "sequence": "contig_1", "start": 800769, "stop": 800903, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACGTGAAGGCAATGAAGAAGAAAATACTTCATTCAAATTAAAATCAATTGAAGAACTTAAAACAAAAGAAAGAAACAAGATTCTAGATATTCAAGAAAACACCAAAAGAACAGCCGGTTATGATAAATGA", "aa": "MSREGNEEENTSFKLKSIEELKTKERNKILDIQENTKRTAGYDK", "aa_hexdigest": "11e3adff4aa866077e77563db5e5371f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5202.721500000002, "isoelectric_point": 6.228837394714356 }, "id": "IGIGPGHMMC_1411", "locus": "GCA000148625_01403" }, { "type": "cds", "sequence": "contig_1", "start": 801192, "stop": 801353, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTACAACAAATAGTTATGTTAAAGCATATAAAGATGCATCTGCATTTATTGATGAATACTACAAAAATGATAATCCATATGCATTCACACAATTTACAATTCCAGGTGTTGCACCTACAAAAATAGCTAAAAAAGGTGAAAAAGAATTAGAATGA", "aa": "MSFTTNSYVKAYKDASAFIDEYYKNDNPYAFTQFTIPGVAPTKIAKKGEKELE", "aa_hexdigest": "10cb30245bb335ec7585f92502734142", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6032.693200000001, "isoelectric_point": 6.175977134704589 }, "id": "IGIGPGHMMC_1412", "locus": "GCA000148625_01404" }, { "type": "cds", "sequence": "contig_1", "start": 801660, "stop": 801971, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAATTAGTTTATGGATCAACCTCTGTTCCCGAAATTGATTTAAAAGCATTATTTGAAAGTATTCAATCTAAATTAGAAACACTATTCCCTGAATTAGTTCCATTAAAACCAAATTATGATGCTACAGCTTCATTAGAAGAAAAAACTAAAAAAGTTGGAGATTACAACAAAAAACTTCTTGACATCATGAGAGATTTTGATAACAACAAGAAGAGTGGTTTATATGATGACAAATTAAAAGAAGAAATCATACCATTAATTGCTAAAGCTTTTGAAGGTGCTGACAAAAAAATAAGAACATTTTGA", "aa": "MPELVYGSTSVPEIDLKALFESIQSKLETLFPELVPLKPNYDATASLEEKTKKVGDYNKKLLDIMRDFDNNKKSGLYDDKLKEEIIPLIAKAFEGADKKIRTF", "aa_hexdigest": "568dd8127204cced78cae8887d949be6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11740.414299999984, "isoelectric_point": 5.226254463195799 }, "id": "IGIGPGHMMC_1413", "locus": "GCA000148625_01405" }, { "type": "cds", "sequence": "contig_1", "start": 802044, "stop": 802403, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATGCTGATCAAGCTAAAAACAGAGCTAAAATGCTTGAAATCTTAAAAAATGATTTTATTTTAAGCAAAAAATACAAATCAATTAATGCAACAAAATACAATGCATTAGATGTAATTTCTAAAAACTTAAAATCAGATTATTATGTAAATAAAGTTTTATTAGAAGATGCCGATTTTGTTAAATATCTCAAAGAACAAGAAAATATTTATGCGCTTGATGCACAAGGCAAAGCAGTAAAAGGTGTTAAATATTCTGATGATGATATTGAAAAATTGAAAAAATTGAATGAAATTAAATATAGAATTAAAGCTGAACAAAACATTTTGGATGTTAATAAGAAATTAACAACTTGA", "aa": "MTDADQAKNRAKMLEILKNDFILSKKYKSINATKYNALDVISKNLKSDYYVNKVLLEDADFVKYLKEQENIYALDAQGKAVKGVKYSDDDIEKLKKLNEIKYRIKAEQNILDVNKKLTT", "aa_hexdigest": "e98b48c37d28520b4023c1b25daab937", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13798.759999999984, "isoelectric_point": 9.187534523010253 }, "id": "IGIGPGHMMC_1414", "locus": "GCA000148625_01406" }, { "type": "cds", "sequence": "contig_1", "start": 802571, "stop": 802735, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAATTAATATTTTCTTTAGGAAGCATTAGCACTTTAACTGCCTTCCCTATAGCTGCTGTTAGCTGTGGCCGTGTTGTTGAAACTCCATCTAAAAAAATTCAAGATGATTTTTTAAAAGCTGGTGCCAGAAAAGAAGAAATCAAAAACATTTGA", "aa": "MKNKKLIFSLGSISTLTAFPIAAVSCGRVVETPSKKIQDDFLKAGARKEEIKNI", "aa_hexdigest": "797d2b7e3082ece398daba3f36b6ffa9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5893.917600000001, "isoelectric_point": 9.806624412536618 }, "id": "IGIGPGHMMC_1415", "locus": "GCA000148625_01407" }, { "type": "cds", "sequence": "contig_1", "start": 803324, "stop": 803452, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATTCAGAAGATGAAGACAAAAAAATAAGTGACATTGAAAAAATTACTGATAATGCATACGAAAATCAATTATTAGGTTATCAAGGCATTAACTTAAACTTTAGCGATTACAACTTGAAATGA", "aa": "MKDSEDEDKKISDIEKITDNAYENQLLGYQGINLNFSDYNLK", "aa_hexdigest": "cc3610a8a37e8454c2c150de08afd4c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4913.2951, "isoelectric_point": 4.2393590927124025 }, "id": "IGIGPGHMMC_1416", "locus": "GCA000148625_01408" }, { "type": "cds", "sequence": "contig_1", "start": 803648, "stop": 803902, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAAAGAATCAAATGAAAAAGAAACTGAAAAAGTTAAACTTCTTTCATTCAAAGGCACAATCTTCCAAAATCATCTTAAAAAATTAGCAACCTTATTTGCTTTAAAAGATTCTTCATTAATTGATACAGCAGAAACTGCATTCACTAAATTAGGTTACAAACTTAAAGTAGAAAATGATGCATTAAAAGAACTACTTAAAGGCGAAAAATTTGTTGAAGGTGTAAAAGACAAAGACTCTGACAAAAAATAA", "aa": "MPKESNEKETEKVKLLSFKGTIFQNHLKKLATLFALKDSSLIDTAETAFTKLGYKLKVENDALKELLKGEKFVEGVKDKDSDKK", "aa_hexdigest": "b773bfcecfb907389672d069d87d669b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9518.931799999997, "isoelectric_point": 9.072845268249512 }, "id": "IGIGPGHMMC_1417", "locus": "GCA000148625_01409" }, { "type": "cds", "sequence": "contig_1", "start": 803913, "stop": 804248, "strand": "+", "frame": 3, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "RefSeq:WP_013354921.1", "SO:0001217", "UniParc:UPI0001E32FB6", "UniRef:UniRef100_UPI0001E32FB6", "UniRef:UniRef50_A0A4P1QFY6", "UniRef:UniRef90_C4XF75" ], "nt": "ATGCAAAACATATTTCAATTAATAATTAACAAAAAAGCAGAAGCAAATATTATCTTTGAAGATGATAAATGTATAGCTTTTTATGACAAATTTCCAATTCAACCTGGACATTTCTTAGTGGTTCCAAAAAAACACAGTAAAAACATTACTGAAGCAGATGATGAAACAGCTGCGCACTTAATAAATGTTGCTCGGAGACTTGGAAAAAAACATGTATTAGATAAGAAAATTGCAGGCTTCAAAATAATAATTAATACAGGTGAAGCTGCTGATCAAACAATTTTCCATACACATGTACACGTAATTCCATATCGTGAAAAAGTACAAGATAACTAA", "aa": "MQNIFQLIINKKAEANIIFEDDKCIAFYDKFPIQPGHFLVVPKKHSKNITEADDETAAHLINVARRLGKKHVLDKKIAGFKIIINTGEAADQTIFHTHVHVIPYREKVQDN", "aa_hexdigest": "246cb95fb7c76e394960a45bb4fe103d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FB6", "ncbi_nrp_id": "WP_013354921.1", "uniref100_id": "UniRef100_UPI0001E32FB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB6", "RefSeq:WP_013354921.1", "UniRef:UniRef100_UPI0001E32FB6" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FB6", "uniref90_id": "UniRef90_C4XF75" }, "psc": { "uniref90_id": "UniRef90_C4XF75", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_A0A4P1QFY6" }, "pscc": { "uniref50_id": "UniRef50_A0A4P1QFY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P1QFY6" ], "product": "HIT domain-containing protein" }, "genes": [ "hinT" ], "id": "IGIGPGHMMC_1418", "locus": "GCA000148625_01410" }, { "type": "cds", "sequence": "contig_1", "start": 804446, "stop": 804721, "strand": "+", "frame": 2, "gene": null, "product": "DNA-binding protein HU", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74", "UniRef:UniRef50_A0A0D5ZK80", "UniRef:UniRef90_C4XF74" ], "nt": "ATGACCAAAAAAGAATTTGTACAAAAAATTGCTGAAATGTTAGAAGTTCCAGTTAGAGTAGCAGATCAATACTTTGATGCATTTCTATTTGTTTTGAAAGAACAACTTATAGCTGAAGACAAAGTTCAACTTTCAGATTTAGGAACCTTCGAAACAAAAATTAGAAGAGGGCGTGAAACAATTAATCCATTTACAAATGAACCAATTCAAGTACCTGAAAAACGTGTTGTAAAATTCACACCTTCAAAATACTTAAGAGATATTGTAAACTTCTAA", "aa": "MTKKEFVQKIAEMLEVPVRVADQYFDAFLFVLKEQLIAEDKVQLSDLGTFETKIRRGRETINPFTNEPIQVPEKRVVKFTPSKYLRDIVNF", "aa_hexdigest": "752c415395f60aa5b520b05f65e60a9d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32FB7", "uniref100_id": "UniRef100_C4XF74", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74" ] }, "ips": { "uniref100_id": "UniRef100_C4XF74", "uniref90_id": "UniRef90_C4XF74" }, "psc": { "uniref90_id": "UniRef90_C4XF74", "product": "DNA-binding protein HU", "uniref50_id": "UniRef50_A0A0D5ZK80" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZK80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZK80" ], "product": "Viral histone-like protein" }, "genes": [], "id": "IGIGPGHMMC_1419", "locus": "GCA000148625_01411" }, { "type": "cds", "sequence": "contig_1", "start": 804746, "stop": 805222, "strand": "-", "frame": 1, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "EC:3.1.21.10", "KEGG:K03700", "RefSeq:WP_041593953.1", "SO:0001217", "UniParc:UPI0002E80786", "UniRef:UniRef100_UPI0002E80786", "UniRef:UniRef50_F9UJF5", "UniRef:UniRef90_A0A3P8LHU8" ], "nt": "ATGCAAAAAAATAGAGGAATGTTATTAGAAAAAATAATTAATAAAACAATTGATTACTATGCCAAAAATAAATTAGCTTATATTGAAAAGAAGACCTTACCTGTTAAGTTTAAGAAGGTTGAAAATGATAATTTGAAATTGGATGAGGCTTATATATTAAAGAAGAGTACAGTTGATTACATTGGTTGTTATAAAGGTTTTTTTGTTGCTTTTGAAGCAAAAAGCACAAATGAAAAAAGATTGCCAGCAAATAATATTGCCCGGCACCAAATTAAGTATTTATTGGATATTGAAAGTAATAGTGGATTAGCTTTCTTTATTATATTTTTTAGTTTTTGTGATGAGTTTTATCTTGTTAAAGCAAGTGAGTTAATTCAGCATATGAATAAATCTATTTCTTATGAAGAAATAAAAAACATAGGTTTTAGAATCGAATTAACCTTTCCAGGTATAATCGACTTTTTGCCCTATGTTTAA", "aa": "MQKNRGMLLEKIINKTIDYYAKNKLAYIEKKTLPVKFKKVENDNLKLDEAYILKKSTVDYIGCYKGFFVAFEAKSTNEKRLPANNIARHQIKYLLDIESNSGLAFFIIFFSFCDEFYLVKASELIQHMNKSISYEEIKNIGFRIELTFPGIIDFLPYV", "aa_hexdigest": "d2fb0302373e00b0c5b61ccedc46c087", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0002E80786", "ncbi_nrp_id": "WP_041593953.1", "uniref100_id": "UniRef100_UPI0002E80786", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002E80786", "RefSeq:WP_041593953.1", "UniRef:UniRef100_UPI0002E80786" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002E80786", "uniref90_id": "UniRef90_A0A3P8LHU8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LHU8", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_F9UJF5", "kegg_orthology_id": "K03700" }, "pscc": { "uniref50_id": "UniRef50_F9UJF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UJF5" ], "product": "Holliday junction resolvase RecU" }, "genes": [ "recU" ], "id": "IGIGPGHMMC_1420", "locus": "GCA000148625_01412" }, { "type": "cds", "sequence": "contig_1", "start": 805360, "stop": 805698, "strand": "+", "frame": 1, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63", "UniRef:UniRef90_C4XF72" ], "nt": "ATGAAATATAGAAAGATAATATTAGGAACTTTGTTAGCAGGCACAATTGTAATTTCGACAAGTACAATTGCTTTTAATATTCAAAGAACCAAGGTTGCAAAGGTTAAAAGCAATGATCCTGCATCAATAGGATCAAAATTTTGTATCAAAGTATCCGGTGCAGTTGCCTTTGCTAAAAATTATTATTTCGAAAAGCCTATTTATTTAAAAGAATTATTAGATATGGCCCAACCTTATGAAAATGCAGACTTAAGCAAAATTAACTTAAAAAAAGAATTAAAAGAAAATCTTAATATTTACATTCCATATAAAAAAACTGATAAACATTTTTTGTATTGA", "aa": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKINLKKELKENLNIYIPYKKTDKHFLY", "aa_hexdigest": "7d916d7a2f2f5445756a1cdd407f84d8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 805360, "stop": 805878, "observations": { "mutation": [ 805696 ], "stop": [ 805696 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF72", "query_cov": 1.0, "subject_cov": 0.6511627906976745, "identity": 0.955, "score": 205.0, "evalue": 4.87e-66, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKIDLKKELKENLNIYIPYQKTNNPFLYWNNLSKEHFKLLVDFGIKKSIVNKILDLREKKNQITWNDLENISGVGKVTLKKLQGILIL" }, "paralog": false, "identity": 0.9418604651162791, "score": 313.0, "evalue": 4.07e-111, "description": "Internal stop codon at 805696. Nonsense mutation around 805696.", "psc": { "uniref90_id": "UniRef90_C4XF72", "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF72", "UniRef:UniRef50_A0A3P8MF63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63" ], "product": "Competence protein ComEA" } }, "id": "IGIGPGHMMC_1421", "locus": "GCA000148625_01413" }, { "type": "cds", "sequence": "contig_1", "start": 806031, "stop": 806408, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTATATTTAAGTTATTTTTTATCTAAAACAAAAATTTATTATGAAAAAGGTATTTATAAAATTGAAGGATCGATAAGCGTCCTAAGCAAAAAATATTTAGTTATATCAAATGAAAATAATAATATTTTAATTTACACAAGTGATTTAAAAAATAGCACTCCTTTAAGTCTAGGGGACCAAGTAGAAATTCAAGGTGAATTAAATCCTATTGAAATAGACAATAGTAAAATAGCAACATTTTATTTGCAAAATAATATAAATTATTTGCTAAAAAAAACAGTCATTTTAACTGTTATTAAACCAGAATTTTCTGTTGAAGAAAAAATAATGAATTATTCAAAAAATCATGGTGAATTATTTGATTCATATTGA", "aa": "MTLYLSYFLSKTKIYYEKGIYKIEGSISVLSKKYLVISNENNNILIYTSDLKNSTPLSLGDQVEIQGELNPIEIDNSKIATFYLQNNINYLLKKTVILTVIKPEFSVEEKIMNYSKNHGELFDSY", "aa_hexdigest": "d3284bc71307502056f8a749a9564334", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14449.421499999986, "isoelectric_point": 5.824996376037597 }, "id": "IGIGPGHMMC_1422", "locus": "GCA000148625_01414" }, { "type": "cds", "sequence": "contig_1", "start": 806412, "stop": 806930, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTTTTGGATCCTATGATTCAAAAGAATTAGTATTGAATAAAGTTAATAGACTTAATATTGTTCATTTAATTGTTATTTCAGGTTTGCATTTTGACTTATTATTCTATTTAATTTTGTTGATACTAAAACCTTTGGATAAAATTAAAATCAAAAGTAAATATATGGCTTTTGCCCTCATATTCTATTATTTAACATTACTTGCTAATCCTGTTTCTGCTTTAAGAGCTTATATTATGCAAGTTGTTAAAAATTCAAATAATCCTTTTAGTTATAACAACAAATTTAAAAAATATGATGGTCTAGTAATTTCGATTTTAGTTTTATTTTTACTTAAAAATAATTACTTGTTTTCTTTATCATTTATTCTTAGTTTTTCATCAACTTTTACAATTATGTTATTAGTGCCAATGGTCAAAAGTAAAAAATTAAACAACTTTTTAAAAACATTAATTTTGATGACTTTAATATATCTTTTTAATATTCCAATGCTTTTATATATTAATGAATATTGA", "aa": "MLVFGSYDSKELVLNKVNRLNIVHLIVISGLHFDLLFYLILLILKPLDKIKIKSKYMAFALIFYYLTLLANPVSALRAYIMQVVKNSNNPFSYNNKFKKYDGLVISILVLFLLKNNYLFSLSFILSFSSTFTIMLLVPMVKSKKLNNFLKTLILMTLIYLFNIPMLLYINEY", "aa_hexdigest": "e2398aa7a53d56e94ac6a367ec5b707b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20074.19740000001, "isoelectric_point": 9.810234642028806 }, "id": "IGIGPGHMMC_1423", "locus": "GCA000148625_01415" }, { "type": "tRNA", "sequence": "contig_1", "start": 807236, "stop": 807312, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 69.4, "nt": "GTACCCATAGCTCAACTGGACAGAGTGTCAGGTTACGGCCCTGAAGGTTGCGGGTTCGACTCCTGCTGGGTACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 807276, 807278 ], "id": "IGIGPGHMMC_19", "locus": "GCA000148625_01416" }, { "type": "tRNA", "sequence": "contig_1", "start": 807317, "stop": 807391, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 69.0, "nt": "GACACCATAGCCAAATGGCCAAGGCATGAGTCTGCAAAACTCTGATTACGGGTTCGAATCCCGTTGGTGTCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 807366, 807368 ], "id": "IGIGPGHMMC_18", "locus": "GCA000148625_01417" }, { "type": "cds", "sequence": "contig_1", "start": 807637, "stop": 808443, "strand": "-", "frame": 2, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2", "UniRef:UniRef90_C4XF70" ], "nt": "TTGGACTTAAATGAAGCTTTAAAAGAATTTGATTATTTAATTTTAGCTGTACCTTCAAGTGCAATTACTTCTGTGTTAGAACAAATAAAGGAAATAATCAAAAATAAAAAAATAAATGTTATTAATGTTGCAAAAGGTATTGATGAAAAAACAAATGAATTTTTTTCAGAAGTTTTAAACAAACAATTTGCGAATAATTTAAAAAATTATTGTACTTTAATTGGTCCTTCTTTTGCTGTTGAAGTTTTTGAAAATGTTTTAACAATGATTAATGTTGTTGGTCCAAAAACAAGATTTTTAAAAAAAGTTTCTGAATTATTTAATAATGACACTTTTAGACTTGTTGTTAATGAAAATGAACACGGTTCAGAATTGTTTGCTGCTCTAAAAAATGTTCTAGCTATTGGAATTGGAATGATTGATTATTTAAGACCATATCGAAATACAATGGCGGCCTTGCTTTCAATTGGTGTTAAAGAAATTCACATGGTTTACAAGAGAATATTTCCTGAATCAAATGACAATATGGGATTTGAATTAGCGGGAATTGGCGATATCTTTTTAACTTGTTCAAGTACAAAAAGCCGTAACTTTTCTTTTGGTAGACAAGTTGCAGAATTTGGCTTGGAAGCAACTTTAAAAAATAATAAGAAAACTATTGAAGGATATCATGCTGCTAAAATATTAGCTAATATTCTAAAGAAAAATAAAGATCTTAAAATTGTATTTTTAAAAAGCATAATTGATATTTTATATAAAAACAAGAAACCTGAATTACTTTTAGATTTTGTTAAAAAATATAAATAA", "aa": "MDLNEALKEFDYLILAVPSSAITSVLEQIKEIIKNKKINVINVAKGIDEKTNEFFSEVLNKQFANNLKNYCTLIGPSFAVEVFENVLTMINVVGPKTRFLKKVSELFNNDTFRLVVNENEHGSELFAALKNVLAIGIGMIDYLRPYRNTMAALLSIGVKEIHMVYKRIFPESNDNMGFELAGIGDIFLTCSSTKSRNFSFGRQVAEFGLEATLKNNKKTIEGYHAAKILANILKKNKDLKIVFLKSIIDILYKNKKPELLLDFVKKYK", "aa_hexdigest": "7e176617dfa6b0775e7b4fa7ae5d64c8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF70", "query_cov": 0.996268656716418, "subject_cov": 0.8042168674698795, "identity": 0.993, "score": 507.0, "evalue": 2.8e-180, "valid": true, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A168RLR2" }, "pscc": { "uniref50_id": "UniRef50_A0A168RLR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "IGIGPGHMMC_1424", "locus": "GCA000148625_01418" }, { "type": "cds", "sequence": "contig_1", "start": 808645, "stop": 808797, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCCATCAAGTATTATGAAAGTGATCAAAAAGAAAAAGCTATTAAATACTATAAAGCATATTGTACAATTGCTTTTAAACGCAAAAAAGATTACAGTGATAAAAATTCTAATAACAAAAATGATGATGACACAATAATAGTTAAATAA", "aa": "MKAIKYYESDQKEKAIKYYKAYCTIAFKRKKDYSDKNSNNKNDDDTIIVK", "aa_hexdigest": "238be7c57edffd201e402de07e650815", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6018.782800000004, "isoelectric_point": 9.352766990661618 }, "id": "IGIGPGHMMC_1425", "locus": "GCA000148625_01419" }, { "type": "cds", "sequence": "contig_1", "start": 808921, "stop": 809070, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTAGATTTAGAATATGAAAAAGAATTAATTGTTGTTTGTAAAAACTATCATGAAGAAGCACAAGCTTATAAAAATGCCAAAAAGTTCATAATTATTTTAGTAACACTTTGCTATCTTATTGCTTTTGGACTTTTTGCGGTTTGA", "aa": "MDLDLEYEKELIVVCKNYHEEAQAYKNAKKFIIILVTLCYLIAFGLFAV", "aa_hexdigest": "20f9c44fba52834201648f7fe98c8b53", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5727.751199999997, "isoelectric_point": 5.090181922912596 }, "id": "IGIGPGHMMC_1426", "locus": "GCA000148625_01420" }, { "type": "cds", "sequence": "contig_1", "start": 809078, "stop": 809854, "strand": "-", "frame": 1, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "EC:2.1.1.182", "GO:0000179", "GO:0006364", "RefSeq:WP_013354928.1", "SO:0001217", "UniParc:UPI0001E32FDA", "UniRef:UniRef100_UPI0001E32FDA", "UniRef:UniRef50_N9V0N0", "UniRef:UniRef90_C4XF68" ], "nt": "ATGTTTCAAGAAAATAAATTAAAAGCCAAAAAAAGATATGGTCAAAATTTTTTAAAAGATCAAAATGTTATTAGCAAAATAATTGAATTGATTAAACCACAAGGTGAAAAGATTTTAGAAATAGGTCCTGGAAGAGGTGCTTTAACTAAAATTTTAAATCAACAAGCAAAAGAATTTACTGCTTTTGAAATAGATCGAGATATGGTTGATTATTTACAAAAAAATAATATCTTAAATCAAAACCAAATTGTCCAAGGAGACTTTTTGATAGCTGATTTGGAAAAATATAAAGACTATGTTGTTGTTGGAAATATTCCTTATTACATAACTAGTGATATTATTTTTAAACTATTAGATTATCGACATAATTTTAAAAAAGCTATTCTTATGGTTCAAAATGAAGTGGCTCAAAGATTAGTGGCTAAACCTAATCAAAATGATTACAGCAAACTTTCAATAACAGTTCAATATTGTGCTGATGTTAAAAAAGAATTATTTGTTAAGAAGACTTATTTTGATCCAGTTCCTAAAGTTGATTCTGCTATTGTTTCTATTACTTTTAAAAATGAAGAAAATGATAATTATGAAAATTTAAAAGACTTTTTCAAATTATGTTTTTTAGCTAGAAGAAAAAAATTATCATTTGCTTTAGCTACAAAATATAGTAATGATAAAATTAAAATTGCATATAATAAATTAAATTTAAAAGAATTAACTAGAATTCAAGAATTAGATTTAAAAACTATATTAGCTTTATACGATTGCTTAGAAAATTAG", "aa": "MFQENKLKAKKRYGQNFLKDQNVISKIIELIKPQGEKILEIGPGRGALTKILNQQAKEFTAFEIDRDMVDYLQKNNILNQNQIVQGDFLIADLEKYKDYVVVGNIPYYITSDIIFKLLDYRHNFKKAILMVQNEVAQRLVAKPNQNDYSKLSITVQYCADVKKELFVKKTYFDPVPKVDSAIVSITFKNEENDNYENLKDFFKLCFLARRKKLSFALATKYSNDKIKIAYNKLNLKELTRIQELDLKTILALYDCLEN", "aa_hexdigest": "6516226d7133019f55b8b2f2b6aff5db", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001E32FDA", "ncbi_nrp_id": "WP_013354928.1", "uniref100_id": "UniRef100_UPI0001E32FDA", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDA", "RefSeq:WP_013354928.1", "UniRef:UniRef100_UPI0001E32FDA" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FDA", "uniref90_id": "UniRef90_C4XF68" }, "psc": { "uniref90_id": "UniRef90_C4XF68", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_N9V0N0", "go_ids": [ "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_N9V0N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V0N0" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "IGIGPGHMMC_1427", "locus": "GCA000148625_01421" }, { "type": "cds", "sequence": "contig_1", "start": 809838, "stop": 810041, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01026.27" ], "nt": "TTGCCAATTGATAGAATATTAACTGAAACTGATGCTCCTTTTCTTTCTCCAGCTCACAAAAGAGGTGAATTAAATTATCCAAATTATGTAATTCAAACAACTCACTTTATTGCAGGTATTAAAAAAATACCAATTGAAAAATTTACTGACCAGATTTTAAAAAATGCAAAGGATTTATTTAAGATAAATGTTTCAAGAAAATAA", "aa": "MPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK", "aa_hexdigest": "6f4369414dd83fa7ff7eea226a90ebd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01026.27", "name": "TatD_DNase", "length": 61, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.1416374110781412e-16, "score": 56.94118118286133, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 7738.960099999998, "isoelectric_point": 9.62998104095459 }, "id": "IGIGPGHMMC_1428", "locus": "GCA000148625_01422" }, { "type": "cds", "sequence": "contig_1", "start": 810135, "stop": 810611, "strand": "-", "frame": 3, "gene": "tatD", "product": "TatD DNase family protein", "db_xrefs": [ "EC:3.1.21.-", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_C4XF67", "UniRef:UniRef90_C4XF67" ], "nt": "ATGTCAATAAAATATATTGATGCTCATACTCATCCATTAAAAGAATACTATGATGACAATTATCATGTAATTGAAAGAGCTCATGCTAAAGGTTTAGTAGCTTTATTAATTACCGGATGTAATGAACAAGAAAATGAAGAAGTTATTAAAATTGCCAAAAACTTTTCATATACTTTTCCTGTTATTGGAATTCATCCAAATGAATGTCATGGAAAGGTTGACGGCGAAATTATTGAAAAGCAATTAGATGATTCTGTTGTTGCAATTGGCGAAATTGGTTTAGATTACTTTTATACACCAGAAAAGAAAGATATTCAAATTGAATCATTGCATGCTCAAATTCAAGTAGCTAAAAAACACAATTTACCAGTAGTAATTCACATGCGTGATGCATATGAAGATTTGTACGAAATTATTAAACAATATTATAAAGATGTTGTTTTTATGATTCATACATATAGTGGTAACTTATATTGA", "aa": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLY", "aa_hexdigest": "ddfea2fffa4234f3aed689ee36d5ef5a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "tatD" ], "truncated": "5-prime", "pseudogene": { "start": 809838, "stop": 810611, "observations": { "mutation": [ 810044, 810137 ], "stop": [ 810044, 810137 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF67", "query_cov": 1.0, "subject_cov": 0.6147859922178989, "identity": 1.0, "score": 323.0, "evalue": 1.42e-110, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLYWAKKFYELGCYFSFSGVATYKNGTETIEVLDWLPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK" }, "paralog": false, "identity": 0.9922178988326849, "score": 519.0, "evalue": 3.76e-190, "description": "Internal stop codon at 810044, 810137. Nonsense mutation around 810044, 810137.", "psc": { "uniref90_id": "UniRef90_C4XF67", "product": "TatD DNase family protein", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_C4XF67", "kegg_orthology_id": "K03424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF67", "EC:3.1.21.-", "UniRef:UniRef50_C4XF67", "KEGG:K03424" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF67" ], "product": "TatD DNase family protein" } }, "id": "IGIGPGHMMC_1429", "locus": "GCA000148625_01423" }, { "type": "cds", "sequence": "contig_1", "start": 810715, "stop": 811659, "strand": "-", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "GO:0003924", "GO:0005525", "GO:0006400", "SO:0001217", "UniRef:UniRef50_A5IXJ1", "UniRef:UniRef90_C4XF66" ], "nt": "ATGGCACTGCCAGGTGAATTTAGTCGACGTTCATTTTTGAATGGTAAAATGGACTTAATTAAAGCTGAAGCAATTAATGATTTAATACATGCCAAAACATTAAAACAAAGTGAATTAGCCATTAAGAAATTTGATGGCAAAACTTCAAACATGATTAAATCATTTATTGACGAATTAGCCTATTTAATAGGTCAAATGGAAATAAATATAGATTATCCAGAGTATGAAGATTTTGAAAATGTTTTGACAAAAGATTTAATTTCTAGATTGCAAAAATTGCAAGACAAATTGAGCTTAATTATCAAAGAAAGTGAAAACTCAAGATTAATTTTCGATGGCATTAAAGTTGCTATTCTTGGTAAGCCAAATGTTGGAAAAAGTTCAATATTAAATTGCTTAATAAATGAAGATAAAGCAATAGTTACAGATCAGGCTGGAACAACTCGTGATCTTGTTGAAGCTTCATATCAAATTGATGGTTTATTATTCAAATTAGTTGATACCGCCGGAATTAGAAAAACCAATCGAAAAATTGAAAAAATAGGTATTGAAAAATCTTTTGAACAAATTGAAAAATGTGATGTTGTAATTCATGTTAATGAAGCTAATACTTTAGAAAATGATTACGATAAAAAAGTTGAAAAATTAGCTAAACAATTTAACAAACCATTAATTAAAGTAATTAATAAAGTTGATTTATTAAAAAATAATAAAAAGAAAAAGAATGTTATTTATGTCAGTGCTAAAAATAAAAACATTGATGATTTAAAGGAAGCATTAGTTAATATTTTTTCAAAGAATGAAATTAACAATGAAGAATACGTTGTTAATGCAAGACAACTAGCATTGGTTAAAAAGGCTCATTCAAGTATTTCTGATGCAATTGAATCAATAAATAATGGCATTGATGTTGATGTAGTAATTATTGATGTAAGACAAGCTTGA", "aa": "MALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQA", "aa_hexdigest": "d6cd6218eb82f148bc43dd1d7491eff9", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmE" ], "truncated": "both", "pseudogene": { "start": 810631, "stop": 811959, "observations": { "mutation": [ 810717, 811773 ], "stop": [ 810717, 811773 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF66", "query_cov": 1.0, "subject_cov": 0.6977777777777778, "identity": 1.0, "score": 587.0, "evalue": 3.83e-209, "gene_start": 1, "gene_end": 314, "reference_start": 109, "reference_end": 422, "reference_sequence": "MYIMINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIAWFIGNKNFTGENTVEINCHGGVLVTNRILELLLANGARMALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQAWADLVDISGRADNELLLDEMFKNFCLGK" }, "paralog": false, "identity": 0.995475113122172, "score": 842.0, "evalue": 2.64e-312, "description": "Internal stop codon at 810717, 811773. Nonsense mutation around 810717, 811773.", "psc": { "uniref90_id": "UniRef90_C4XF66", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_A5IXJ1", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF66", "UniRef:UniRef50_A5IXJ1", "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXJ1" ], "product": "tRNA modification GTPase MnmE" } }, "id": "IGIGPGHMMC_1430", "locus": "GCA000148625_01424" }, { "type": "cds", "sequence": "contig_1", "start": 811771, "stop": 811974, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATGACACAATTGCAGCAATATCTTCAGGTGGTAAGATTAATCAAGCCATATCAATAATCAGAGTTAGTGGTCAAGATAGTATTGAAATAGTTTCTAAAATTTTTAAAGGAAAAATAGGAACAAATCAAACAATAACTTATGGCCATATTTTTGATAATAAAACAAAAGAAATTATTGATGAAGTTTTAATTGCTTGA", "aa": "MINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIA", "aa_hexdigest": "16c3ea807b913ca42a1c14ac7cfdd624", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.255399999998, "isoelectric_point": 6.533664894104004 }, "id": "IGIGPGHMMC_1431", "locus": "GCA000148625_01425" }, { "type": "cds", "sequence": "contig_1", "start": 812045, "stop": 812731, "strand": "+", "frame": 2, "gene": null, "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354931.1", "SO:0001217", "UniParc:UPI0001E32FDC", "UniRef:UniRef100_C4XF65", "UniRef:UniRef50_A0A3P8MDF4", "UniRef:UniRef90_C4XF65" ], "nt": "ATGAATAATCAAAATTTTAAGAATTTATTAACTAAATATATTGAATTCTTTTATAGGTATAAAAGCATTTCATTATTAAAAAAGGCAAATAATGAATCACAATTTGATGTTAAATTAAGATTAGTTTTAACACAAAAATTTTTACTGCATTCACTAGGTTTGCAATACAACAAAAAGTTATTTAGAAAATATAAGAACAATAATTTTGAACTTTATAGAGATATAAAAAATGATCAACTAAATTATGATGAGTTAATTAAAGACATAAGCAAAAACAAAAAAGATTTAATTGACAAAAAATTAAACGCTCTTGAGATTTTTGTCAATATTATAAAAAATATTAATCAAGAAAAACTATTTACTTTTGCATTTGAATTTTTTAAATTAAAACCAAAATTTGATATTGATGGAGCTTATAACCATTCACAATTGATTTTAGTTACAAAAAGCAATAAAGAAAATCAAAGATGTATTATTTATGGAATTTTGGGAGATTTATATACCACCAATTCAAAAGATAATAATCATTTTTTAAACGAAAATAAAAATCTTATGAACACAGTTTTTATACCTATATCAATAAAAATCAAAAACAATGAAAATCTTTTAAATTCTCAAAATATACAAAAAATTGATATTGCATTTTTAAACAAAAGAAGAATACAAGAAAAAGAAATAAGAGTATAA", "aa": "MNNQNFKNLLTKYIEFFYRYKSISLLKKANNESQFDVKLRLVLTQKFLLHSLGLQYNKKLFRKYKNNNFELYRDIKNDQLNYDELIKDISKNKKDLIDKKLNALEIFVNIIKNINQEKLFTFAFEFFKLKPKFDIDGAYNHSQLILVTKSNKENQRCIIYGILGDLYTTNSKDNNHFLNENKNLMNTVFIPISIKIKNNENLLNSQNIQKIDIAFLNKRRIQEKEIRV", "aa_hexdigest": "7616135b0e1370eea83caffd82d75333", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDC", "ncbi_nrp_id": "WP_013354931.1", "uniref100_id": "UniRef100_C4XF65", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDC", "RefSeq:WP_013354931.1", "UniRef:UniRef100_C4XF65" ] }, "ips": { "uniref100_id": "UniRef100_C4XF65", "uniref90_id": "UniRef90_C4XF65" }, "psc": { "uniref90_id": "UniRef90_C4XF65", "uniref50_id": "UniRef50_A0A3P8MDF4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDF4" ], "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1432", "locus": "GCA000148625_01426" }, { "type": "cds", "sequence": "contig_1", "start": 812769, "stop": 813092, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGCCTGATCCAAATGCTTTAACAGAAGTTAAATCAACTTCAAGTGCTTCTGGTGAAGAAACTCCAACAGATTCGAAATTAAATAACCTAAGTCTAAAAGCAAAAGCTATTGTTGAAGCTGTTGGTGGTTACGAAAACATTACAACTTTTGCAAACTGTGCAACAAGACTTCGTTATGAAGTTAAAGACTCAGATATTGTTGATGAAGCAAAATTAAAACTTGCGGGATGCAGTGGTTTGATTAGATTAGGAAAGAATTCAATTCAAATTATTGTAGGTGTTGAAGCTGAAAACATTAACAATGAAATTGAATCAAATAAATAA", "aa": "MPDPNALTEVKSTSSASGEETPTDSKLNNLSLKAKAIVEAVGGYENITTFANCATRLRYEVKDSDIVDEAKLKLAGCSGLIRLGKNSIQIIVGVEAENINNEIESNK", "aa_hexdigest": "5af06d39b2de6278ce5dade57c7f8897", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 34, "aa_cov": 0.3177570093457944, "hmm_cov": 1.0, "evalue": 1.5064878251883422e-16, "score": 55.58298110961914, "start": 35, "stop": 68 } ], "seq_stats": { "molecular_weight": 11426.709099999993, "isoelectric_point": 4.8286657333374015 }, "id": "IGIGPGHMMC_1433", "locus": "GCA000148625_01427" }, { "type": "cds", "sequence": "contig_1", "start": 813195, "stop": 813539, "strand": "-", "frame": 3, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "nt": "ATGTTTGGTCTTCCTGCTCTAGCATTTGCTTTCTACATGACTGCAGATGATAAAGTGCAAAAGAGAAGAGTAATGGCTATCTTTGGAGCAGCCGCAGTAGTATCAATTCTTTCAGGTATTACTGAACCTATCGAATTTGCATTTATGTTTGTTAGTCCATTATTATATGGTTTCCACTGCATTCTAACTGGTATATTTGCATTTATAACTGGTGCATTTGGAATTCAAATTGGATTTGGGTTCTCAGCTGGTTTAATGGATTACTTATTATCAATTCCTAAATCATTAGCAATTATTAAAGCTAACAAAACAGGTATTGATGCTGTATTAGCTAACCCTGGTTGA", "aa": "MFGLPALAFAFYMTADDKVQKRRVMAIFGAAAVVSILSGITEPIEFAFMFVSPLLYGFHCILTGIFAFITGAFGIQIGFGFSAGLMDYLLSIPKSLAIIKANKTGIDAVLANPG", "aa_hexdigest": "d33d0bc54d09b9fc48e069f20f333793", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0016429E17", "query_cov": 0.8070175438596491, "subject_cov": 0.9019607843137255, "identity": 0.5329999999999999, "score": 91.3, "evalue": 9.15e-22, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0035A088D1" }, "pscc": { "uniref50_id": "UniRef50_UPI0035A088D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "IGIGPGHMMC_1434", "locus": "GCA000148625_01428" }, { "type": "cds", "sequence": "contig_1", "start": 813577, "stop": 813801, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCCTTTTGCTTTACCACTTAAGAAAATAAAGATATCACCAAATACAGGTGTACCATCAGGAGTTACATGTTCTCCTAATTGGAATCAGAATAAGTTGTTTGGAATGTGGTGTAATCCAAATGGAATTAATAGACGATTAATAACGCCATATACACCCATAATAGCAGCTGAAGCTCAACGACCTTTAGCTGCACCTGATAACACTTTTGAAAATGCATAA", "aa": "MFPFALPLKKIKISPNTGVPSGVTCSPNWNQNKLFGMWCNPNGINRRLITPYTPIIAAEAQRPLAAPDNTFENA", "aa_hexdigest": "08b603faac097bd854914613f42c5a16", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8155.374299999995, "isoelectric_point": 9.589817237854003 }, "id": "IGIGPGHMMC_1435", "locus": "GCA000148625_01429" }, { "type": "cds", "sequence": "contig_1", "start": 813846, "stop": 814523, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTACAAAAACACAATCATCAAGACCTTCATTTGGAAAAAGACTATTAGAAAAACTTGGGCGCATTGGTCGGGTATTGATGTTCCCTATTGCAGTTCTTCCTATTGCTGCTATTCTTCTTAGAGTTGGAGCTGATTTACCAACAGTTAAAGCTGGACCTGATATTTCACAAGTCGAAGCTAACTTTGTTAACTTTGTTCAACAAATGATAACAGCAGGCGGATCAGTTGTATTTGACAACTTGTACATTTTATTTGCCGTCGGAATAGGATTTGGTTTTACTAAAGATAACCGTGGTGAAGCTGCTTTTGCTGCTTTAATTAGTATTCTATTACTTTCACTTTTATTAAAAAGTGGTGGATATGTAGATAAAATCTACAGCACATTTAAACTCCCTGGAGCCGAATTTAATGAAGATGGTAAAAAACTTGAAGCTTTTATAGCTGAAGGTAAACCTGCTTCAGAATTTGTTATAGCTAATGGTTTTGCAGGTGTCTTTGGTGGCAAATACAATGCAATAATGTCTGGCAATGTACTTAATGGTATTATTGTTGGATTTATTGTTGCAGCTATTTATAACCATACAAATGATGTTCAATTACCTAAAGTTCTTGGATTCTTTTCGGGTAGAAGATTAGTTCCAGCTCTTGCAATTCTTTCAACTCTTATTTGA", "aa": "MTGTKTQSSRPSFGKRLLEKLGRIGRVLMFPIAVLPIAAILLRVGADLPTVKAGPDISQVEANFVNFVQQMITAGGSVVFDNLYILFAVGIGFGFTKDNRGEAAFAALISILLLSLLLKSGGYVDKIYSTFKLPGAEFNEDGKKLEAFIAEGKPASEFVIANGFAGVFGGKYNAIMSGNVLNGIIVGFIVAAIYNHTNDVQLPKVLGFFSGRRLVPALAILSTLI", "aa_hexdigest": "eab4b7a4d83c5d0a21045785c86786e9", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23917.7978, "isoelectric_point": 9.58169422149658 }, "id": "IGIGPGHMMC_1436", "locus": "GCA000148625_01430" }, { "type": "cds", "sequence": "contig_1", "start": 814523, "stop": 814726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_258408850.1", "SO:0001217", "UniParc:UPI0002EEF8E6", "UniRef:UniRef100_UPI0002EEF8E6", "UniRef:UniRef50_UPI0002EEF8E6", "UniRef:UniRef90_UPI0002EEF8E6" ], "nt": "ATGGTTTTAATTATTATCTTAGCTTCTATTAAGAACTTCGATGCTGCAGAATCTTTAAAAACAACAAATGCTAAATACGTAGCCATTGTTGCCTTATTTTTCTTTATATCTTTATGTGCTGCAATTTTTGGAACAGTTATTTTATCAATCTTAGATAAAAAAGCTGTTACAAAAAAAGCACAACAAAAACAAGGAGGCAAATAA", "aa": "MVLIIILASIKNFDAAESLKTTNAKYVAIVALFFFISLCAAIFGTVILSILDKKAVTKKAQQKQGGK", "aa_hexdigest": "3bea24394627c01cd31549cfab347272", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002EEF8E6", "ncbi_nrp_id": "WP_258408850.1", "uniref100_id": "UniRef100_UPI0002EEF8E6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002EEF8E6", "RefSeq:WP_258408850.1", "UniRef:UniRef100_UPI0002EEF8E6" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002EEF8E6", "uniref90_id": "UniRef90_UPI0002EEF8E6" }, "psc": { "uniref90_id": "UniRef90_UPI0002EEF8E6", "uniref50_id": "UniRef50_UPI0002EEF8E6" }, "pscc": { "uniref50_id": "UniRef50_UPI0002EEF8E6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EEF8E6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7217.662999999999, "isoelectric_point": 9.84659481048584 }, "id": "IGIGPGHMMC_1437", "locus": "GCA000148625_01431" }, { "type": "cds", "sequence": "contig_1", "start": 815038, "stop": 815739, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013354934.1", "SO:0001217", "UniParc:UPI0001E32FDF", "UniRef:UniRef100_C4XF62", "UniRef:UniRef50_C4XF62", "UniRef:UniRef90_C4XF62" ], "nt": "ATGAAGAAAAACTTATTAAAGTTAACAGGAATTATTGCTCCAGTTATTACAGCTATACCATTTGTTGCTTCATCATGTGGTAGTAAAGATGAAGCAACTTTAGCTGATCTTTTAAAGGAAAAATTGACAAAGGATGCAGATGCTTCTAAATATTCTGCACTAATTACTGACAAGGAAAAATTAAAACAATTCAAGGAATTAGTAGAAGAATTTAAAAAAGAAGGAGTAGATTTAACTGCTAAAGGTAATCCTTCTGATGTAGATAATGCAATTAAAACTATTAAAGAAAAAGCGCCATTAGTTTTAGCTAGAACAATTAAAGCAATGGTTGAAAACGGAAGTGCTTTTATAATTAAAGAAGATGGGATTGCTAGTGCTAAACAAAAACTAGAAAATGGTGATATATTTGCTCAAGTTTATCCTGCACAACATAAAATCGGAAGATCTGCTTATACAATTATTGAATTTACTGAAAAATTTAAGGCATTTTATTTAACTTCAAAAGTATTCGAAGTTGCTAGAATTTCAACATTTTATGAAAATAAATATGGTGGTAAAACTCAACCTCATGCAAACGGAGTAAACTTAGAATTTGTTAAAGGCAAGTTAAATATTAAATTTGTGATTTGTGATTTGCTAAAAGGCGACCAAACTGGTTCAGTTGTTTTAGTAAATAACTTATTATTTGCAGTTGAAAAATAA", "aa": "MKKNLLKLTGIIAPVITAIPFVASSCGSKDEATLADLLKEKLTKDADASKYSALITDKEKLKQFKELVEEFKKEGVDLTAKGNPSDVDNAIKTIKEKAPLVLARTIKAMVENGSAFIIKEDGIASAKQKLENGDIFAQVYPAQHKIGRSAYTIIEFTEKFKAFYLTSKVFEVARISTFYENKYGGKTQPHANGVNLEFVKGKLNIKFVICDLLKGDQTGSVVLVNNLLFAVEK", "aa_hexdigest": "4ad2567d9b653639e138017a546a76b7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDF", "ncbi_nrp_id": "WP_013354934.1", "uniref100_id": "UniRef100_C4XF62", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDF", "RefSeq:WP_013354934.1", "UniRef:UniRef100_C4XF62" ] }, "ips": { "uniref100_id": "UniRef100_C4XF62", "uniref90_id": "UniRef90_C4XF62" }, "psc": { "uniref90_id": "UniRef90_C4XF62", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XF62" }, "pscc": { "uniref50_id": "UniRef50_C4XF62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF62" ], "product": "Lipoprotein" }, "genes": [], "id": "IGIGPGHMMC_1438", "locus": "GCA000148625_01432" }, { "type": "cds", "sequence": "contig_1", "start": 815908, "stop": 816324, "strand": "-", "frame": 2, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "ATGACAACAGTTCACGCTTACACAGGTGACCAAATGTTACAAGATGGTCCACACCGTAAAGGTGACTTAAGACGTGCTCGTGCTGCTGCTCAAAACATTGTTCCTTCAACAACAGGTGCTGCAAAAGCTATTGGTTTAGTTGTTCCTGAAGCTGATGGATTATTAGATGGTTCAGCATTGCGTGTTCCTACAATTACAGGTTCATTCGTTGACTTAACAGTTCAATTGAAAAAACAACCTACAGTTGAAGAAATTAATGCTGCATTCAAAAAAGCAGAAAACGAAACAATGAAATATGTAGTTGACCCTATCGTTTCTTCAGACGTTATTAACTCACACTATGGTTCATTATTCGATTCAACTTTAACAAAAGTTAAAGAAGGAAATGGTCAAAGACTTTACAAAATCTTTGCATGA", "aa": "MTTVHAYTGDQMLQDGPHRKGDLRRARAAAQNIVPSTTGAAKAIGLVVPEADGLLDGSALRVPTITGSFVDLTVQLKKQPTVEEINAAFKKAENETMKYVVDPIVSSDVINSHYGSLFDSTLTKVKEGNGQRLYKIFA", "aa_hexdigest": "6b35d1e376449254ce947b31e6efdcc8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 1.0, "subject_cov": 0.8679245283018868, "identity": 0.703, "score": 192.0, "evalue": 1.95e-60, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "IGIGPGHMMC_1439", "locus": "GCA000148625_01433" }, { "type": "cds", "sequence": "contig_1", "start": 816589, "stop": 816846, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAGTTGCAATCAACGGTTTCGGTCGTATTGGACGTTTAACACTTCGTCACTTACTTGAAACCCAAAATAAAGAAGTTCAAGTTGTTGCAGTTAACGACTTAACAGATCCAAAAATGTTAGCACATTTACTTAAATACGATACAGCTTTTGGCCCATTGGAAGCTAAGGTTGAAGTTAAAGAAAATGCTATTAAAGTTAATGGAAAAGAAATTAAAGTATTCTGCGAAAAAGATCCAGCTAACTTACCATGA", "aa": "MKKVAINGFGRIGRLTLRHLLETQNKEVQVVAVNDLTDPKMLAHLLKYDTAFGPLEAKVEVKENAIKVNGKEIKVFCEKDPANLP", "aa_hexdigest": "c073492bc640cadb5a1b7ec5609d0565", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9764705882352941, "subject_cov": 0.8469387755102041, "identity": 0.6509999999999999, "score": 104.0, "evalue": 1.29e-27, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 83, "aa_cov": 0.9764705882352941, "hmm_cov": 0.9759036144578314, "evalue": 5.888438955445813e-31, "score": 102.83222198486328, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9498.060999999992, "isoelectric_point": 9.131253623962404 }, "id": "IGIGPGHMMC_1440", "locus": "GCA000148625_01434" }, { "type": "cds", "sequence": "contig_1", "start": 817333, "stop": 817533, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACCAATTTTAGTAAGATTATTAAATGGAATTTATGCTGTTAGTTGTGCATTACAAATAGTATTGCCTACAAAAATAAAAGAAGTTGCAAGTGTTTTAAAAGTTAAAAAATTTGACTTTGAAAAAATTAATGATTTCAATAAATTTGATAAAGTTATTTGTGATGAGAAATATTTGTTATTTAATAGATTAAAATAA", "aa": "MEPILVRLLNGIYAVSCALQIVLPTKIKEVASVLKVKKFDFEKINDFNKFDKVICDEKYLLFNRLK", "aa_hexdigest": "609aac8a8f3aae22747e0ef7c4f0caa4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7704.206999999998, "isoelectric_point": 9.23040599822998 }, "id": "IGIGPGHMMC_1441", "locus": "GCA000148625_01435" }, { "type": "cds", "sequence": "contig_1", "start": 817558, "stop": 817953, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAATCTTTAAATAATGTTATAGCCCCAGATTATTTATTTGACAAGTATCATCATGAGATGATTAAATACTATTTTGCAACTCAAATAATATTTGGAGAAGATGGTAATTTTAGTGAAGAAAAATTAAAAGAAGTTGTTAATGCAGATTTAATAAATAATTATGGTAATTTAGTTTCAAGAACACTAAAAATGATTCACAATTCTTTCCCAAAAGGTCTGTTTTATAGTGTTAATAGTAAAAAAATACATAAAGACATTGAAAAAGAAATTAAGAATTTCAGTGCTCAATTTCAAAAACATATGGACGCTTTTGAAATTGATAAAGGATTTAAAAAAGCAATTGAATTATCAGCTAAATTAAATAAATATATTGATGAAACAACACCATGA", "aa": "MSKSLNNVIAPDYLFDKYHHEMIKYYFATQIIFGEDGNFSEEKLKEVVNADLINNYGNLVSRTLKMIHNSFPKGLFYSVNSKKIHKDIEKEIKNFSAQFQKHMDAFEIDKGFKKAIELSAKLNKYIDETTP", "aa_hexdigest": "a8fe5b07687517abca7a1b70b13e25cd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15291.311999999985, "isoelectric_point": 7.874249076843262 }, "id": "IGIGPGHMMC_1442", "locus": "GCA000148625_01436" }, { "type": "cds", "sequence": "contig_1", "start": 818782, "stop": 818877, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAAAGACTTTTTATATAACTACTCCTATTTATTATGCTTCAGGTCCGCTTCATATCGGACATCTTTATTGTACAATTATGGCTTGA", "aa": "MKKQKTFYITTPIYYASGPLHIGHLYCTIMA", "aa_hexdigest": "47b9a5c2cc8114a57fd64ec4c252bdb6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3591.2688000000007, "isoelectric_point": 9.361470222473145 }, "id": "IGIGPGHMMC_1443", "locus": "GCA000148625_01437" }, { "type": "cds", "sequence": "contig_1", "start": 818997, "stop": 819665, "strand": "-", "frame": 3, "gene": null, "product": "Methyltransferase small domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_C4XF58" ], "nt": "ATGAATAAAAAACAAAAATTAGTTAAAAATTCTTTAGGTTTTGATTCTAATTTATATGTTGTGCAAGACAAAGATATGTTCAACTATTCAGTTGACACTATTATGCTTGGCAACTTTGTTTTTTTAAATAATAAAATTAAAAACCTATTAGAAATTGGAGCAAACAATGGAGCTCTCAGTATTTTTATAGCTGAAAGAAATCCAAATTTAAAAATAGATGCAGTTGAAATCCAAACTAAAGCTTGTAAGTTAGCTCAAGACAATATTGAACTTAACAACAAGCAAAATCAAATCAAAATAATTAATCAAGATTTCAATGAATTTTATTTAGAAAAAATTAAAAATGCAGCTAGTAAATATGACTCAATAGTTTGCAATCCTCCTTTTTATACAATGCAAACTAATAAAGTTAGCAAAAATATTAGTGAAGAATTACTAATTGCAACACATGAGTATAAAATTAATTTAGAACAAATTATTTTAGGTTCTTCAAAATTAATTGAGCAAAAAGGCTACTTAACTTTAGTGATTCCAGTTGAACGCTTAGTTGATTGCTTTTGCTTAATGAGAAAATATAATTTTGAGCCTAAAAGAGTTCAATTCATTATTCCAAGAATGCAAGATAAACCTAAATTAGTTTTAGTTGAAGGCAGATATCAGGCTGGTTGA", "aa": "MNKKQKLVKNSLGFDSNLYVVQDKDMFNYSVDTIMLGNFVFLNNKIKNLLEIGANNGALSIFIAERNPNLKIDAVEIQTKACKLAQDNIELNNKQNQIKIINQDFNEFYLEKIKNAASKYDSIVCNPPFYTMQTNKVSKNISEELLIATHEYKINLEQIILGSSKLIEQKGYLTLVIPVERLVDCFCLMRKYNFEPKRVQFIIPRMQDKPKLVLVEGRYQAG", "aa_hexdigest": "bc2ae9df8dc10fd8e62d00e2fbd2b347", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XF58", "query_cov": 1.0, "subject_cov": 0.844106463878327, "identity": 1.0, "score": 432.0, "evalue": 1.84e-152, "valid": true, "product": "Methyltransferase small domain-containing protein", "uniref50_id": "UniRef50_A0A448ZXG4" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [], "id": "IGIGPGHMMC_1444", "locus": "GCA000148625_01438" }, { "type": "cds", "sequence": "contig_1", "start": 819668, "stop": 821851, "strand": "-", "frame": 1, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "RefSeq:WP_013354939.1", "SO:0001217", "UniParc:UPI0001E32FE3", "UniRef:UniRef100_UPI0001E32FE3", "UniRef:UniRef50_A5IXZ7", "UniRef:UniRef90_C4XF57" ], "nt": "ATGAATCCGCAAGAAATATTAAATTTTTTAAAAAAACATGATAATTCAACTTTCATTGATATTGCAAAAGGCTTAAGAATAAGTTATAAAGATAACAAAAAATTAACCGAATTACTAAATAAATTAATTGATGAAAGAAAATTAATTAAAAATGGCAAACTCTCAACTTATTCATTAATAGAAAAAATTAAAATCGTCCAAGGAAAAATTCGATTTGCTTCAGAAGGCAAGTATGCTTTTGTAGAAGAAGAAAATGATAATCCTGAAGCTGAAAAAATAAGTTATTTTGTACCTGGTATTCATCTTAATAGAGCATTAGATGGAGACTTTGTTGAAATAACAGTTTTAAAATACTTTCATTCTTTAGATGATAAAACTTTTGCAATTGTTGAAAAAGTTTTAAGCAGAAACAGTGATAAATTCATTGGTATTTTAGGTATGCATGAAGGTTTTCTTTCATTTAAACCACTTAAAAATGACTACAAAAAAATTAATTTTCATATTAAAGAATTGGTTAAAGAAGCTAGACTTCATGATGTTGTTTTAGGTCAAATGGTTTCATTTAAAAATAATATTATTGAGCTTAACATTATCAAAAAAATAGCTAACAGTAATGATCCAATGGCTTATGTTAAAGCCTTAACAGTTATTAGAAATGAACCTGAAGATTTCCCACAAGAAGTTAAAGATTACTTAGTTAAAATTCCAAATAGTATTGCAGATGAAAAACTTAATAATAGACTTGACTTGAGAAAAGAATTAATTGTTACTATTGATGGTAATGACACAAAAGACTTTGATGATGCAATTAATGTTAAAAAGAATGCTGATGGAACTTATGAATTAGGCGTTCATATTGCTGATGTTAGTTATTATGTCAAAGAAGACAGCTCTCTTGATGTTGAAGCATTAAAAAGAGGAACCTCAAGATATTTAGTTAGTGGCGTTATTCCAATGTTGCCTCATAAATTATCAAATGGTATTTGCTCACTTAATCCTAATGAAGATCGCTTTACTCTTAGTGCAATAATGACAATTGATAAAAATGGTGATACTATTGATGCTAAATTTTATCAGTCAATAATTCAATCAAAATATCGTCTCACATATGATAGGGTTAATGAACTTTATGATAAAAATCAAAAATTTGATGACCCGAAATTAAATCGAATGCTCTATGAAGCAGCTGAATTGGCAAAAATAATTAGAAATTATAAAGTCAAAGAAGGCTACATTGATTTTGAAATTGAAGAACCCGAAATTGTCTTAGATGATAAAGGTAAAACAATTGATGTTGTAGTTAAGCCAACTGGTTTTAGTGAAAATCTTATTGAAGATTTCATGGTTAGAACTAATGAAGAAGTAGCTAAATTTTTAGCTAAAAGAAAAATACCTTTTATTTATCGTGTTCATGAAAGCCCTGATAGTGACAAAATTGATTCATTTTTGAGTGTACTTAATGTTTTAGGTATTAAAGTAAACATTGATCGAAATGATATTAATCCAAAAAGTTTTCAAAAAATAATCAACAAAATTAAAGAAACAAGAAATGATGACTTTTTAAAAACAGTTTTCTTAAGAACAATGCAAAAAGCTAAATATTCTCCAAATAATATTGGCCACTTTGGTTTAGCAAGTGAACACTATTGTCACTTTACTAGTCCAATTCGACGTTATCCAGATTTAGTTGTTCATAGGATTTTAAGAGAAATTGTTTTTAACAATGATAAAAGTAAAATTACTCACTACAAAAACATTTTAAGTAATGTTGCAGAATTAAATTCAAATAGTGAACAAGAAGCATTACAACTTGAACGTGATACAAATGATTTAAAATATGCTGAGTTTTTCAAAAACAAAATTGGTCAAACAATGAAAGCTCAAATTATTAGTATTTTACGCTTTGGTATGTTTGTCCAATTTGACTGCAAAATAGAGGCTTTAGTTCATATTTCAACATTATGTGATGGTGATTATGAAGTTAATGAAAACTTTACTGAATTAAGAAGTAAACAACACACTTTCAAACTTGGAGACCAAGTAGAAGTGGTTATTGCTGGAGCTGATGAAACTAACGGTAAAATTGATGCAGTGCTTGCAAAATGCTATGGTTCATACTTAGAAGAAATCAAGAAAAAACTTAATTCAAACTCAAATCGCAAACACAAAAAATCAGGTAAATAA", "aa": "MNPQEILNFLKKHDNSTFIDIAKGLRISYKDNKKLTELLNKLIDERKLIKNGKLSTYSLIEKIKIVQGKIRFASEGKYAFVEEENDNPEAEKISYFVPGIHLNRALDGDFVEITVLKYFHSLDDKTFAIVEKVLSRNSDKFIGILGMHEGFLSFKPLKNDYKKINFHIKELVKEARLHDVVLGQMVSFKNNIIELNIIKKIANSNDPMAYVKALTVIRNEPEDFPQEVKDYLVKIPNSIADEKLNNRLDLRKELIVTIDGNDTKDFDDAINVKKNADGTYELGVHIADVSYYVKEDSSLDVEALKRGTSRYLVSGVIPMLPHKLSNGICSLNPNEDRFTLSAIMTIDKNGDTIDAKFYQSIIQSKYRLTYDRVNELYDKNQKFDDPKLNRMLYEAAELAKIIRNYKVKEGYIDFEIEEPEIVLDDKGKTIDVVVKPTGFSENLIEDFMVRTNEEVAKFLAKRKIPFIYRVHESPDSDKIDSFLSVLNVLGIKVNIDRNDINPKSFQKIINKIKETRNDDFLKTVFLRTMQKAKYSPNNIGHFGLASEHYCHFTSPIRRYPDLVVHRILREIVFNNDKSKITHYKNILSNVAELNSNSEQEALQLERDTNDLKYAEFFKNKIGQTMKAQIISILRFGMFVQFDCKIEALVHISTLCDGDYEVNENFTELRSKQHTFKLGDQVEVVIAGADETNGKIDAVLAKCYGSYLEEIKKKLNSNSNRKHKKSGK", "aa_hexdigest": "aae83234080e788b7c78be493e38af4c", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001E32FE3", "ncbi_nrp_id": "WP_013354939.1", "uniref100_id": "UniRef100_UPI0001E32FE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FE3", "RefSeq:WP_013354939.1", "UniRef:UniRef100_UPI0001E32FE3" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FE3", "uniref90_id": "UniRef90_C4XF57" }, "psc": { "uniref90_id": "UniRef90_C4XF57", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_A5IXZ7", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXZ7" ], "product": "Ribonuclease R" }, "genes": [ "rnr" ], "id": "IGIGPGHMMC_1445", "locus": "GCA000148625_01439" }, { "type": "cds", "sequence": "contig_1", "start": 821855, "stop": 822091, "strand": "-", "frame": 1, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "RefSeq:WP_013354940.1", "SO:0001217", "UniParc:UPI0001E32FE4", "UniRef:UniRef100_C4XF56", "UniRef:UniRef50_A0A4Y6I792", "UniRef:UniRef90_C4XF56" ], "nt": "ATGGCAGTTATATTTGTATCAATAATTATCGCTATTTTATCGATCGCAATTATTGTGATTTCATTGCTTATGTCACCAGACAGTAATGGTTTTTCTGGTGCACTTGTTGGTAGTGGTGATTTGGAACTTTTTAAATATTCAAAAGAAAGAGGACTTAAAAAGGTTTTAAAATACTCAATGTTATTTGGTGGAATAGCATTGCTAATTTTAGCTATTATTTTAAGGATTATTGTATAA", "aa": "MAVIFVSIIIAILSIAIIVISLLMSPDSNGFSGALVGSGDLELFKYSKERGLKKVLKYSMLFGGIALLILAIILRIIV", "aa_hexdigest": "9aa2fc4addf0534ccd25b6c256a57b1c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FE4", "ncbi_nrp_id": "WP_013354940.1", "uniref100_id": "UniRef100_C4XF56", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FE4", "RefSeq:WP_013354940.1", "UniRef:UniRef100_C4XF56" ] }, "ips": { "uniref100_id": "UniRef100_C4XF56", "uniref90_id": "UniRef90_C4XF56" }, "psc": { "uniref90_id": "UniRef90_C4XF56", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_A0A4Y6I792", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I792", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I792" ], "product": "Protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "IGIGPGHMMC_1446", "locus": "GCA000148625_01440" }, { "type": "cds", "sequence": "contig_1", "start": 822305, "stop": 823237, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55", "UniRef:UniRef90_C4XF55" ], "nt": "ATGAAAAACAAAATAACCAAACACAATATGTGTGAAATTTATGAGTCATTTCGTTTTATTCAACCTGAATATAAAAGTCGTGTAAATTCATTAAAAAATAAAAATGAAAATGACGAATTTTTAATCAAAGCACAAATGGAAAGTGCTATTTCATTGAATGAATATATTGATTCAAAATTAGAAAGAAAAAAGATCAGAATTGCTAATCGTAAGATTTCAAAAATCTCAAGTATCTTTATTCCACTAATTGTTTTCTTCTTAGTAACTGTTTTAATTGCAGTGTCTTATATTGCTTATATAGCAGCTAACTATATGGTTAAAGATGCTGAAGTTTATACACTATTTGGTATAGCTATATTATCACTTATTGCAGCAGCAATATGCTTTGTAGTTTACTATAGAAAATACACGCGATATAACAATGATAAGGCTAAATATTTTGAAGATAAATTAAAAGAAGAAATCAAAATTGGAGATTTAAAATCTATAGTTTTAAAAAACAAAAATACATTAAATTATCAAGAAGATGTTGCTTCATTAGTTGTTGCTAACGATCTAAAATATCCTGATATTCCTGCAGGCTACAAAGTTGAAGCTTCAAAATACGCTAAACGTTTTACACTATATGGAATGAATGTTTTAGCACAAGATGCTCAACTAGTAAAAATTGACGAAAAGGCTAATGAAACAGCTGAATCAGACAAAAGTATTATTAGTAATAAAGCTAAAAAAATTAAGATTTTTGAATTCTCATTTATGAATAGTCAATTTGACAAAATTTATTTACCATTTAACTTATTTAACAAATTTGAAGTTTTCAAAGCTAAACTTGAACCAATCAAAAGTAACGTTTCTGATTTAGACAAAATCTTCAATATTCATGCAATCAAAAACCCGCTTAACAGTAAACTTTTCGATGCTCTTAAATTATGA", "aa": "MKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYIAANYMVKDAEVYTLFGIAILSLIAAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKL", "aa_hexdigest": "d19e975f92793fef33b4563677cd34cc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 822290, "stop": 823540, "observations": { "mutation": [ 823235, 823376, 823439 ], "stop": [ 823235, 823376, 823439 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF55", "query_cov": 1.0, "subject_cov": 0.7110091743119266, "identity": 0.9940000000000001, "score": 575.0, "evalue": 7.27e-205, "gene_start": 1, "gene_end": 310, "reference_start": 6, "reference_end": 315, "reference_sequence": "MYGVSMKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYVAANYMVKDAEVYTLFGIAILSLISAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKLWKNNIHFAHTFIPQESGVNYFNFKLTAIFTLKEECEDEFNIQTNAKYWNNPFKVELPETIDDSTKLPMWFIINNFYKMVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI" }, "paralog": false, "identity": 0.9855769230769231, "score": 805.0, "evalue": 1.12e-297, "description": "Internal stop codon at 823235, 823376, 823439. Nonsense mutation around 823235, 823376, 823439.", "psc": { "uniref90_id": "UniRef90_C4XF55", "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF55", "UniRef:UniRef50_C4XF55" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35910.52660000003, "isoelectric_point": 9.37848987579346 }, "id": "IGIGPGHMMC_1447", "locus": "GCA000148625_01441" }, { "type": "cds", "sequence": "contig_1", "start": 823466, "stop": 823600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGACTATATTGCACTTTCAAAAGCAACAGTTGAAAAATATGTTATGCCAATGATATTCTACTTATTTAATGAAATTAATGACACCAAAGTATTTAATGAAAAAACTCTTTCAGATTTATACGAAATTTAA", "aa": "MVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI", "aa_hexdigest": "6448879fe5c36b8893f9243f0f1e2b57", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5241.0548, "isoelectric_point": 4.440682792663575 }, "id": "IGIGPGHMMC_1448", "locus": "GCA000148625_01442" }, { "type": "cds", "sequence": "contig_1", "start": 823690, "stop": 824298, "strand": "+", "frame": 1, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "EC:2.7.4.8", "GO:0004385", "GO:0006163", "SO:0001217", "UniRef:UniRef50_Q4A6S7", "UniRef:UniRef90_C4XF54" ], "nt": "ATGAATATTATGAACAATTTAATTAGCAAACGTAAGCCAATAATAATATTTACAGGACCTAGCGGAGTTGGCAAAGGAACAATTGAAAAGTACCTTTTTGATTTTAAAAATTTAAAATTACATTTATCATGTTCAGCAACAACTAGAGCTCCTCGTGAAGGTGAAATTAATGGCATTCATTACTTTTTTATAACAAAAGAAGAATTTGAAAAACACATTCAAAATGATGATCTTTTTGAATACAGTTTTCATTTTGATAATTATTATGGAACACTTTATAGTGAAATTGCAAGAATTCATAGAAATGGCGAAATTCCTGTTTTAGAAATTGAAACCAATGGTGCAAAACAAATATTAGAAAAGGTTTCAAATAACAAGAATTACAAATTGATCACACTTTTTATCTTACCTCCTTCAATTGAAGAACTTGAAAGAAGAATTATTAACCGAAAGACAGAAAGTCAAGAAGCTATTAAAAAACGTTTAGCTAAAGCGCAAGCAGAAATAGCAGAAAAAGGAATATTTAGACATCACATAATTAATGATGACCCTTTAAGAGCTGCAAAAGAAATAAAAGAAATTATAGAGAAAGAATACGGTATCAAATAA", "aa": "MNIMNNLISKRKPIIIFTGPSGVGKGTIEKYLFDFKNLKLHLSCSATTRAPREGEINGIHYFFITKEEFEKHIQNDDLFEYSFHFDNYYGTLYSEIARIHRNGEIPVLEIETNGAKQILEKVSNNKNYKLITLFILPPSIEELERRIINRKTESQEAIKKRLAKAQAEIAEKGIFRHHIINDDPLRAAKEIKEIIEKEYGIK", "aa_hexdigest": "d2c1cadc72c5d57840c14dfdae4cea65", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF54", "query_cov": 1.0, "subject_cov": 0.9528301886792453, "identity": 1.0, "score": 397.0, "evalue": 9.73e-140, "valid": true, "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q4A6S7", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S7" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "IGIGPGHMMC_1449", "locus": "GCA000148625_01443" }, { "type": "cds", "sequence": "contig_1", "start": 824298, "stop": 824522, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGGAATAACAAGCGACATGGGAAGAGTAAGGTTGGAAAACCAAGACAAAGCTAGTTACTTTACAAAAGGTAACTATGTTTTATTAATTTTATGTGATGGAATGGGTGGTCACTATGGTGGTGGGATTGCTTCAAGGACAACAATAAATACTTTTAATAAAATTTTTAACAATTCAATTCCAGCTGAAAATTCTGAAATTAAAGATTATGTATCTTGA", "aa": "MEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVS", "aa_hexdigest": "60c8b73bca8efe0ae567b04e59b7b3b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8188.092699999998, "isoelectric_point": 5.636006736755371 }, "id": "IGIGPGHMMC_1450", "locus": "GCA000148625_01444" }, { "type": "cds", "sequence": "contig_1", "start": 824556, "stop": 825065, "strand": "+", "frame": 3, "gene": null, "product": "Serine/threonine phosphatase stp", "db_xrefs": [ "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_A0A449B4I9", "UniRef:UniRef90_A0A3P8KWN9" ], "nt": "ATGATTAAAATTAGTGCTAATGATGAAGCAAAACTAGACATGGGAACAACAGTTACAGCTGCCTTACTTAATACAAAAACAAAATTATTATTAATATTTAATATTGGCGATTCACGTACTTATGCTATGACAACTTTTGGTGAATTAAAGCAAATTTCAATTGATCACAACTTATACAATAAGTTAATTAATGAAGAAGGTAAAAGTCCTTATGAGGCTAAATTGAATCGTTTCCATGCTTCATTAACTTCAGCTTTAGGACCTGATAAAAAAACTAAAATTGAAGTTTTTGACTTAAGTAGTCAATACGAACATGTTTATAGTTTATTAGTCACAAGTGATGGTGTACACGACTTTATCGAAAAACCAAAAATGGAAATGATTTTAAGGGAAAATCTTAATGCTGAAGACATGACTAAAAAATTAGTGGAAGAGGCTTTAGATAATAAGTCAACAGATAACTGCTCAGCAGGTATGGTCATATTAGACAATCTAAAAGAATGGAGTTAA", "aa": "MIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVEEALDNKSTDNCSAGMVILDNLKEWS", "aa_hexdigest": "5413dec3e5d2e871dd9bb13dcf7864e0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 824283, "stop": 825065, "observations": { "mutation": [ 824520 ], "stop": [ 824520 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KWN9", "query_cov": 1.0, "subject_cov": 0.65, "identity": 0.988, "score": 326.0, "evalue": 2.12e-111, "gene_start": 1, "gene_end": 169, "reference_start": 92, "reference_end": 260, "reference_sequence": "MRYQIMEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVSWFKKTVDASRDEMIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVKEALDNKSTDNCSAGMVVLDNLKEWS" }, "paralog": false, "identity": 0.9846153846153847, "score": 510.0, "evalue": 2.05e-186, "description": "Internal stop codon at 824520. Nonsense mutation around 824520.", "psc": { "uniref90_id": "UniRef90_A0A3P8KWN9", "product": "Serine/threonine phosphatase stp", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_A0A449B4I9", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KWN9", "EC:3.1.3.16", "UniRef:UniRef50_A0A449B4I9", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B4I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4I9" ], "product": "Serine/threonine phosphatase stp" } }, "id": "IGIGPGHMMC_1451", "locus": "GCA000148625_01445" }, { "type": "cds", "sequence": "contig_1", "start": 825067, "stop": 825870, "strand": "+", "frame": 1, "gene": null, "product": "Protein kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5", "UniRef:UniRef90_C4XF52" ], "nt": "ATGGGAATTAAACCACAAAGTAATATATTTAAGAAATATCGTGTGCTATCAACTATTGGTGATGGTGGTTTTAGTCAAGTTTATAAAGTTGAACTTTTAAATGATAAAAGCCCAATTAAAAGAAAGTTTGCACTTAAATATAGTGTTAATAAAAATAAAAATGATGAAGACATAACTCGTAGAAGATTTGAACAAGAAATCACGATATACAAAAAATTAAATACTGAACGTGTGGCATTATACTTTGACTCTTATAGCGATGAATATGAACAATATCTTGTAATGGAATTTGTTGAAGGACAAAATTTAAGAGAAATAATTAAAAGAAATGGTAAATTCACTACAACAGTTGCTGTTAACTATGCAATTCAAATAGCTGAAGGAATTAGTGAATTACATAACTTAGGTGTAATTCACCGTGATATTAAAAGTAATAACATTTTAATTACCAAAGATAAAAATGTAAAAATTATTGACCTTGGCCTAGCTTTAGATGATGAAAGTCAAAGACTTACACAAGAAAACAAAGTAGTCGGTTCTGTTTTCTATATGGCTCCTGAAATTTGTTTAGCTAATTCGAAACCATCTGTCCGTTCGGACATTTATGCTTTAGGCATTTTGTTATTTGAAATGCTAACTGGCTCATATCCTATTTCAGGTAAAGACCAACAAGAAACTTTAAGAATGCAAAGAACAACAAATGTTCCTAACTTATTAAACTTTGTTAATGCACCTCAAGCTTTAGCTAACGTTATCATCAAAGCAACAGCTAAAGATCCAAACAAAAGATATGCAACTATGTGA", "aa": "MGIKPQSNIFKKYRVLSTIGDGGFSQVYKVELLNDKSPIKRKFALKYSVNKNKNDEDITRRRFEQEITIYKKLNTERVALYFDSYSDEYEQYLVMEFVEGQNLREIIKRNGKFTTTVAVNYAIQIAEGISELHNLGVIHRDIKSNNILITKDKNVKIIDLGLALDDESQRLTQENKVVGSVFYMAPEICLANSKPSVRSDIYALGILLFEMLTGSYPISGKDQQETLRMQRTTNVPNLLNFVNAPQALANVIIKATAKDPNKRYATM", "aa_hexdigest": "c48c69eb7c2b6a589beb00b9fb77313e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_C4XF52", "query_cov": 1.0, "subject_cov": 0.8042168674698795, "identity": 1.0, "score": 517.0, "evalue": 2.94e-184, "valid": true, "product": "Protein kinase domain-containing protein", "uniref50_id": "UniRef50_A0A449B4H5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B4H5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5" ], "product": "Serine/threonine protein kinase" }, "genes": [], "id": "IGIGPGHMMC_1452", "locus": "GCA000148625_01446" }, { "type": "cds", "sequence": "contig_1", "start": 825874, "stop": 826065, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTCGTGATCTTATGACTTGTCTTGATACTAAAAGAGTTTATGAAAAACCTCTTGATCTTAGAAAAGTTAAACCTAAAAACACAATTCAAGAAAAAGTTAATAGCAAGAAATTTATTATTGCAGGTATTTCAATAATATTAGTTGTTTTGATTATAGCTTTAGTATTGATTGTTGTTTATGTTCACTAA", "aa": "MRRDLMTCLDTKRVYEKPLDLRKVKPKNTIQEKVNSKKFIIAGISIILVVLIIALVLIVVYVH", "aa_hexdigest": "8ad71a6af2053b45477d0ecbc3bec0a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.9175, "isoelectric_point": 10.008668327331542 }, "id": "IGIGPGHMMC_1453", "locus": "GCA000148625_01447" }, { "type": "cds", "sequence": "contig_1", "start": 826078, "stop": 826491, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_L0RWK0", "UniRef:UniRef90_C4XF51" ], "nt": "TTGAAAGGCAAAATTTATTCAATTAATTCAGGTAAATATTACATAAATGATTTGCAAAATAAAATGCACATATTACCTGCAGCTGGTGTTTTTCGTCACAAAGAAATAACACCTTTAGTGGGTGATATTGTTGAGTTTGAAGAAGGCCAATATATCAAAGAAATATATCCTAGAAAAAATTCTTTTGTTCGTCCAAAAGTAGCAAACATCGACAATATTGTTGTGGTTATGTCAATTAAAGAACCTGAATTTCAAAGTTACTTAGTTGACAAATATTTAGCTTTTATAGAAAGTAAAAATATCAAACCAATTATCATTTTGACTAAAGATGATTTAGGTTCAACCCCTTACTTTGAACAATATAAAAATATGGGTTATGAAATTTACCAAATAAACTATAAAATAAATGATTGA", "aa": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKIND", "aa_hexdigest": "4ec913c3114f4729477e10c9da87fa97", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [ "rsgA" ], "truncated": "3-prime", "pseudogene": { "start": 826078, "stop": 826794, "observations": { "mutation": [ 826489 ], "stop": [ 826489 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF51", "query_cov": 1.0, "subject_cov": 0.49280575539568344, "identity": 1.0, "score": 279.0, "evalue": 2.7e-93, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKINDWVKIFTKIFDHKTCSLMGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE" }, "paralog": false, "identity": 0.9915966386554622, "score": 473.0, "evalue": 6.73e-172, "description": "Internal stop codon at 826489. Nonsense mutation around 826489.", "psc": { "uniref90_id": "UniRef90_C4XF51", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_L0RWK0", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF51", "EC:3.1.3.100", "EC:3.6.1.-", "UniRef:UniRef50_L0RWK0", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWK0" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "IGIGPGHMMC_1454", "locus": "GCA000148625_01448" }, { "type": "cds", "sequence": "contig_1", "start": 826537, "stop": 826914, "strand": "+", "frame": 1, "gene": null, "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "nt": "ATGGGTCAAAGCGGAGTTGGAAAAACTACAATAATTAATAAATTAACCAACAATAATTATGAAACTCAAAACATTTCAAAATCAGTAAATCGGGGTAAACATACCACAAGAATTGTTCAAATAATTCCAATATTAAATGGTGAATTAATTGACACTCCAGGCTTTAGCTCTTTTGATTTAAACATCTCAAAATTAGAACTTGCTTCTTCTTATTCTCAATTTAGAGAACTAGGAAAATTATGTAAATTTAAATCATGCTTGCATGAAAATGAACCTGAAGAATATTGCAATATAAAACTCAATGTAAAAAACAAAACAATACCAGAATTTAGATATCAAAATTATCTAAAATTATTAAAGGAGATTAAACATGAATAA", "aa": "MGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE", "aa_hexdigest": "54255a8149dd775762fd9cdb3e183f00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013679F51", "query_cov": 1.0, "subject_cov": 0.8865248226950354, "identity": 0.552, "score": 144.0, "evalue": 6.83e-42, "valid": false, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_UPI0013679F51", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013679F51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "product": "ribosome small subunit-dependent GTPase A" }, "genes": [], "id": "IGIGPGHMMC_1455", "locus": "GCA000148625_01449" }, { "type": "cds", "sequence": "contig_1", "start": 826907, "stop": 827008, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAAAAATATGTAACACCTTCATTATTAAATGTTGAAAAAGATAAACGTTGTGAAATGGCAACACTATTAGTTAATAATGGAATTAAATGA", "aa": "MNKKEKYVTPSLLNVEKDKRCEMATLLVNNGIK", "aa_hexdigest": "7d565ecce1ab86c360dfabbc5514f727", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3808.4932, "isoelectric_point": 9.360438728332518 }, "id": "IGIGPGHMMC_1456", "locus": "GCA000148625_01450" }, { "type": "cds", "sequence": "contig_1", "start": 827024, "stop": 827569, "strand": "+", "frame": 2, "gene": null, "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1", "UniRef:UniRef90_C4XF50" ], "nt": "ATGGATGGCAAATTTGTACCTAACACAGCTATTGAATTAGATGAAATTAAAAACATTAACAAGAATGCACCTAAACACTTTAAAGATGCTCACTTAATGGTTGAAAATCCTCTTGATTACTTAGATGAATTTAGAGACCATGTTGATATTACAACAATTCATTATGAAGCAGTTAATAAAAAAGAATTGTTGCAATTCTTAAAAGATCATCATTCAGCTTACAGTATAGGACTTGCTATTAAACCAGCAACCGAAGTTAAAGAAATAGTTAAAATGCTTCCTTATTTACAACTTGTTTTAGTTATGTCTGTAGAACCTGGCAAGGGAGGACAAAAATTTATTGATCATGCTGTTGATAAAATTAGAGAACTTAAACAATTAAGAGACAAAAATGGTTACAAATATTTAATTCAAGTTGATGGTGGAATTAATGGTGAAACTGGACCAAGATGTTTTGCAGCTGGAGTAGATGCAACAGTAGCTGGAACTTATATTGTTTCAGAACCAACTAAGGAAAGAATTGCTAGTGTATTTGGTAAAAAATAA", "aa": "MDGKFVPNTAIELDEIKNINKNAPKHFKDAHLMVENPLDYLDEFRDHVDITTIHYEAVNKKELLQFLKDHHSAYSIGLAIKPATEVKEIVKMLPYLQLVLVMSVEPGKGGQKFIDHAVDKIRELKQLRDKNGYKYLIQVDGGINGETGPRCFAAGVDATVAGTYIVSEPTKERIASVFGKK", "aa_hexdigest": "9576d37dcaf38f6d768b263a830dea33", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF50", "query_cov": 1.0, "subject_cov": 0.8153153153153153, "identity": 0.9890000000000001, "score": 364.0, "evalue": 5.35e-127, "valid": true, "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_Q4A6T1" }, "pscc": { "uniref50_id": "UniRef50_Q4A6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [], "id": "IGIGPGHMMC_1457", "locus": "GCA000148625_01451" }, { "type": "cds", "sequence": "contig_1", "start": 827687, "stop": 827887, "strand": "-", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354947.1", "SO:0001217", "UniParc:UPI0001E32FEB", "UniRef:UniRef100_C4XF49", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_A0A3P8MF76" ], "nt": "ATGTCAAGAAGAGACCAATTAACAGGCAAAGGACCTATGGTTGGAAACATTCGTTCACACGCAATGAATGCTTCAAAAAGAAAATTTAACGTTAACTTGCAAAAAGTAACAATGAATGTTAATGGTAAAAAAGTAACTTTAAGAGTTAGTGCTAAAACAGCAAAAACCTTAAAGAACAAAGGTGTTAGTGTTGTTGCCTAA", "aa": "MSRRDQLTGKGPMVGNIRSHAMNASKRKFNVNLQKVTMNVNGKKVTLRVSAKTAKTLKNKGVSVVA", "aa_hexdigest": "67da65a9a58e544d6c8369b3b20a907f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FEB", "ncbi_nrp_id": "WP_013354947.1", "uniref100_id": "UniRef100_C4XF49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FEB", "RefSeq:WP_013354947.1", "UniRef:UniRef100_C4XF49" ] }, "ips": { "uniref100_id": "UniRef100_C4XF49", "uniref90_id": "UniRef90_A0A3P8MF76" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MF76", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "IGIGPGHMMC_1458", "locus": "GCA000148625_01452" }, { "type": "cds", "sequence": "contig_1", "start": 828148, "stop": 828546, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48", "UniRef:UniRef90_C4XF48" ], "nt": "ATGTTTAGAACTTTAAAAGAAAGATTTATAACATTAACAGTAATGTTTGCTCTTGCTTCATTAATGACTATTATAGCTATGGCATTATTTGGAGTTTCAAGAATTGCATTCGCTACTTTAAGTGGTGTAGTTATAGGAGTAAGAACATCCTTAGGAATGTCAATAGCAGCATTTATCTTAGCAATTATAGCCTTTGTCTTTGTTCTTATTATTTACTTGCTTTACAATAGAATAATGGCAAAACGTACAGAAGATTTTAGTCGTTTTAGACACACAAAAGCATCACAAATTTTTGTTTTAGTAGCTTTAATTTGTTATGCTGTTGCAGCAGTTACAGCAATTTTGTATTTTGTAATGGTAGCTATTGTTCATAATAATAATTCAAGTACAATTAGTTGA", "aa": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIAFATLSGVVIGVRTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTIS", "aa_hexdigest": "01f0e1f81fe3e320827dffe43e6616f6", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 827995, "stop": 828546, "observations": { "mutation": [ 828084, 828105, 828150 ], "stop": [ 828084, 828105, 828150 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF48", "query_cov": 1.0, "subject_cov": 0.7213114754098361, "identity": 1.0, "score": 222.0, "evalue": 3.16e-72, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIAFATLSGVVIGVRTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTISWETVRAFSIVFEIFFWIGIVIGWLFIILYYVFFCIIVDNITRYRRAQARKK" }, "paralog": false, "identity": 0.9836065573770492, "score": 333.0, "evalue": 2.19e-118, "description": "Internal stop codon at 828084, 828105, 828150. Nonsense mutation around 828084, 828105, 828150.", "psc": { "uniref90_id": "UniRef90_C4XF48", "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF48", "UniRef:UniRef50_C4XF48" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14609.583699999981, "isoelectric_point": 10.637750816345214 }, "id": "IGIGPGHMMC_1459", "locus": "GCA000148625_01453" }, { "type": "cds", "sequence": "contig_1", "start": 828559, "stop": 829491, "strand": "-", "frame": 2, "gene": null, "product": "FAD/NAD(P)-binding domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354949.1", "SO:0001217", "UniParc:UPI0001E32FED", "UniRef:UniRef100_UPI0001E32FED", "UniRef:UniRef50_C4XF47", "UniRef:UniRef90_C4XF47" ], "nt": "ATGGACAAAAAAGATATTAAAAAATTTGATGTTTTAATTGTTGGATCAGGACCCGCAGGACTAAATGCTGCTCTTTATACCTCTCGAGCAGGACTAAATACAGCTTTTATTGATAATGATGTCTTTGGTGGCAAAATTCAAAAAACAGCTAAGGTAGATAACTACCTTGGAATTCAAGATATAAGTGGTTTTGACTTATCACAAAAATTTTACGAACATGCCTTAAAATATGGCGCAAAACATCTTTATGGCAAGGTTGTAAAAATCAATTCAATTAATGAAAATTTACAAGAAGCAATTCTTGAAAATGGTGAAATTATTCAAGCTAAAAAATTAATTATAGCTTCAGGTATGATTAATAGATATCCTAAAGGTGTTGATAATTATCAAAGACTTGAAGAATATGTAAGCTTTTGTGCTATTTGCGATGGTGCATTATATAAGAATAGAAAAGTAGTTGTATTAGGCGGCGGAAATTCTGCAGTTGAAGAAGGAACTTTTTTAGCTAAATTATGTACTGATGTAACAATAGTTACAAATCAAAATACATTTAGTGCTGAGGAAGAATTGGTTGAGGAACTCAAAAAGCAAAAAAATGCTAAAATTTATTATGATCATGAATTAATTTCTTTAAATGGTGAAAATCAGTTAACTTCAGTAACTATAAGGAATAAAAAAACCAATAAAGAAATTAATATTGTAGCTGATGGCTTATTTGTTTTCATAGGCTTTATACCAAACCAAGATGCATTTAAGCATCTAAAAATAACAACTAAAGAAGGTTTTGTAAAAACCAATGAAGATATGGAAACAAAAGTAAAAGGCATCTATGCAATTGGTGATATCAGAGATAAAAAAGTAAGACAAATTATTACAGCAGCTGCCGATGGTGCAATAGCTGCAAAACACATTTCTGATACATTAAAAGATTAA", "aa": "MDKKDIKKFDVLIVGSGPAGLNAALYTSRAGLNTAFIDNDVFGGKIQKTAKVDNYLGIQDISGFDLSQKFYEHALKYGAKHLYGKVVKINSINENLQEAILENGEIIQAKKLIIASGMINRYPKGVDNYQRLEEYVSFCAICDGALYKNRKVVVLGGGNSAVEEGTFLAKLCTDVTIVTNQNTFSAEEELVEELKKQKNAKIYYDHELISLNGENQLTSVTIRNKKTNKEINIVADGLFVFIGFIPNQDAFKHLKITTKEGFVKTNEDMETKVKGIYAIGDIRDKKVRQIITAAADGAIAAKHISDTLKD", "aa_hexdigest": "f9801a7df378f9b5c655b8012000b27d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32FED", "ncbi_nrp_id": "WP_013354949.1", "uniref100_id": "UniRef100_UPI0001E32FED", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FED", "RefSeq:WP_013354949.1", "UniRef:UniRef100_UPI0001E32FED" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32FED", "uniref90_id": "UniRef90_C4XF47" }, "psc": { "uniref90_id": "UniRef90_C4XF47", "product": "FAD/NAD(P)-binding domain-containing protein", "uniref50_id": "UniRef50_C4XF47" }, "pscc": { "uniref50_id": "UniRef50_C4XF47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF47" ], "product": "FAD/NAD(P)-binding domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1460", "locus": "GCA000148625_01454" }, { "type": "cds", "sequence": "contig_1", "start": 829814, "stop": 829912, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAGCGATGATATTTCAAAAGGCGCTTATCGTTATCCTCTCTTCTTATATGAATCTCTTGCTAACTTAGTTGGTTACATTGTTTTAGTATGA", "aa": "MFISDDISKGAYRYPLFLYESLANLVGYIVLV", "aa_hexdigest": "194cdaf7206032b834f7b6ca8cb56998", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.260400000001, "isoelectric_point": 4.556577491760254 }, "id": "IGIGPGHMMC_1461", "locus": "GCA000148625_01455" }, { "type": "cds", "sequence": "contig_1", "start": 830000, "stop": 830116, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCTTTGTACTTGACATGATTTATGTTTATACCAAAAGAGATCTAGTTGACATTCGTAAAGTTGCTTCAATTATTATTCCAACAATTCTAATTGGTCAAGTTATTGGACGTTGA", "aa": "MAFVLDMIYVYTKRDLVDIRKVASIIIPTILIGQVIGR", "aa_hexdigest": "327c3a5344c25d6e7f5528bd1bc1e2af", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4335.2679, "isoelectric_point": 9.518902015686034 }, "id": "IGIGPGHMMC_1462", "locus": "GCA000148625_01456" }, { "type": "cds", "sequence": "contig_1", "start": 830430, "stop": 830753, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA", "PFAM:PF07475.17" ], "nt": "TTGGAAGCTAGAGGTATTGGTTTGATTGATATGAAATATACTTATGGAGTTAAGTCAGTAACTGATAATTGTGAAATTGATTTAGTTGTTGAATTAGTAAGTAACCAAACAGAAGAAGCAAAAAATTTAGATCGCTTAGGTCTTTCAGATCTTAAGTTTCCAATTTTAGATGGAGCTTTACCTAAGATTCAAATTCCAGTTAAAGATGGTTCAAGCGCTGCTAGCTTAATCGAAGCAGCCGTAAGTACTTTCTTAGCAAGAAAAGATGGAATCGATGTTTTAAAACAAATACAAGAGAGAGGAGAAAAAGAACATGAATTATAA", "aa": "MEARGIGLIDMKYTYGVKSVTDNCEIDLVVELVSNQTEEAKNLDRLGLSDLKFPILDGALPKIQIPVKDGSSAASLIEAAVSTFLARKDGIDVLKQIQERGEKEHEL", "aa_hexdigest": "b03271c1e924b1cc0e35240526e78ffd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF66EA", "query_cov": 0.9065420560747663, "subject_cov": 0.9509803921568627, "identity": 0.557, "score": 104.0, "evalue": 3.42e-27, "valid": false, "uniref50_id": "UniRef50_UPI0021AF66EA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF66EA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07475.17", "name": "Hpr_kinase_C", "length": 100, "aa_cov": 0.9345794392523364, "hmm_cov": 0.98, "evalue": 2.5323552600255094e-25, "score": 84.97459411621094, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11714.279299999987, "isoelectric_point": 4.730050086975097 }, "id": "IGIGPGHMMC_1463", "locus": "GCA000148625_01457" }, { "type": "cds", "sequence": "contig_1", "start": 830995, "stop": 831219, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATGTAGATGAGTTTTCAGTTGAGAAAACCGGTATTCTATATTTGTTGCAAACCTTTAAAATTCATTTTTTAGGTAACTCTCTAACCCCTTTTGAAAGAATAACTAAAGGCGGTTCTTCTTTAAGGACTCGAGCTAAAATGTTGATAGCTTCTTCTTTACCTTTTTTTGTAAAGAAAATTGATTCACTCGTACCTCAACAAATAATATTTCTTGAATAA", "aa": "MLDVDEFSVEKTGILYLLQTFKIHFLGNSLTPFERITKGGSSLRTRAKMLIASSLPFFVKKIDSLVPQQIIFLE", "aa_hexdigest": "09569d19dd4af3cdedfd82b24b598542", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.863699999998, "isoelectric_point": 9.222669792175292 }, "id": "IGIGPGHMMC_1464", "locus": "GCA000148625_01458" }, { "type": "cds", "sequence": "contig_1", "start": 831195, "stop": 831371, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAAGAAAAATAAATATTAAAGAAATTATTAAGGAATTCGAACTAACAATTTGTAATGAAGAATGTGATCCTATTTACCGAGATGTTCAAGAACCTGCGATTAAAAGATTAGGTTTAGAATTATCAGGTCAAATTGAAAACACTCGTTATTCAAGAAATATTATTTGTTGA", "aa": "MRKRKINIKEIIKEFELTICNEECDPIYRDVQEPAIKRLGLELSGQIENTRYSRNIIC", "aa_hexdigest": "7d727676140ae1a39f1dba749c1b15ad", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6925.002199999999, "isoelectric_point": 7.690281486511232 }, "id": "IGIGPGHMMC_1465", "locus": "GCA000148625_01459" }, { "type": "cds", "sequence": "contig_1", "start": 832430, "stop": 832780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "6a907dccef0cbbba0bbf260e0f56003e", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13576.067399999976, "isoelectric_point": 9.912288093566893 }, "id": "IGIGPGHMMC_1466", "locus": "GCA000148625_01460" }, { "type": "cds", "sequence": "contig_1", "start": 834096, "stop": 834269, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGAAGCATTATGATAAACCATGTTTAGGCTTTTTTACCTTAGATTTTATTAAGAAAAATATAGAAGAGTTCAAACGATATAATTGCTTAGTTTTAAGTTATTTGAATCCTGATAATAACAAATATTATAGTCCTTTAAATGATCGTGAATTATTTGATTATGTATGA", "aa": "MSMKHYDKPCLGFFTLDFIKKNIEEFKRYNCLVLSYLNPDNNKYYSPLNDRELFDYV", "aa_hexdigest": "2aca2aa3ffb96819f2a44ba600bd40e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6994.953500000001, "isoelectric_point": 6.528151512145995 }, "id": "IGIGPGHMMC_1467", "locus": "GCA000148625_01461" }, { "type": "cds", "sequence": "contig_1", "start": 834270, "stop": 834503, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGCTGATATTACTTTAAATAATGATTCTAAATTAAAAGAATTTAACATTACTCCTTTTTATAAATCAGGAGGAGCAAACTTCAGCGGAACAGCTGAAGCTTTATCAAAACAAAATCCTTTTCTTGATATTAAACCAAGTGAAAAAGCAGCTAAATTTTTAGAAGATACAAGTGATAAACCAAAGTATGATGTCAAATTAGAAAATGATTCAAGTAATGAATTTTGA", "aa": "MTLADITLNNDSKLKEFNITPFYKSGGANFSGTAEALSKQNPFLDIKPSEKAAKFLEDTSDKPKYDVKLENDSSNEF", "aa_hexdigest": "6ffd9497766d16633e2882933a498a2a", "start_type": "TTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8566.393699999999, "isoelectric_point": 4.791549873352051 }, "id": "IGIGPGHMMC_1468", "locus": "GCA000148625_01462" }, { "type": "cds", "sequence": "contig_1", "start": 834540, "stop": 834980, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGAAAAATCTTTTGCGAGCACTTGCCCCAATTTATTCCCTACCCTTAGTTACTTTTACAACCTCTTGTAACTTTTCAGTTATTAGTTTCAAGGGTTCAAGTAGCATGATTCAGTATTTAAATGATATTTCTAAAGAATTAGGTTATGAATATGAAATGAATATTGTAGGAGGAGGCAGTGGCACTGGCCTAAGTCAATTAATTCATGGCAAAACTGATTTTGCTTCAATAAGTAAAGATCCACGTTTTGGTTTGAATTCGTCAAAGAAAAAACAAGCTAAAAAAGAATATTTAGAAAATCAACTTAAGACTTTTTTAGTTGCAAAAGAAAATGTTGCTTTTGTACTTAAACTTCCTAAATCAAAGATTCAATTATCAAGTGGAGAAGTTGAAGCAAAAGATTTAAATGTTTATTTTAACAAAAATAATTGA", "aa": "MKKKKNLLRALAPIYSLPLVTFTTSCNFSVISFKGSSSMIQYLNDISKELGYEYEMNIVGGGSGTGLSQLIHGKTDFASISKDPRFGLNSSKKKQAKKEYLENQLKTFLVAKENVAFVLKLPKSKIQLSSGEVEAKDLNVYFNKNN", "aa_hexdigest": "d59e5789d143ab08865f42e83d5e5ab5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16238.613299999986, "isoelectric_point": 9.661248207092285 }, "id": "IGIGPGHMMC_1469", "locus": "GCA000148625_01463" }, { "type": "cds", "sequence": "contig_1", "start": 835453, "stop": 835956, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATATATTTAATATGGAAAATGGGACAAACTTATTTAATGCCACAATGGTCTTAAGTTTAATGTGCATTCCAACTATGATCAATTCATTATTATTGCAATTTGAAAAAGTTGATTTATGTCTAAAACTCTCAGGTAGTGCTATTGGTTTAAGTGAATCAAAAGTAATTTATAAAATAATTAAAAAGTCATTAAACAAAAAAATATTTTTAATTTATATTAGCTCTTTTGCCAGAGTTATCGGTGAATCAGCTGCTTTAATGTTTATTTTAAATAGCAATAATTATTCAGAAAATTTTACAAATATTAAGAAATTTTTTAACTCTTCATTGCGTCCTCTTTCAGTATTTATTAATGCTAATTTCTTTGCTGAAAATGGTGGCGATGCAACTAGAAGTTACATGTTTAATTTAGCTTTTGTTCTATTAATTGTGATAACTTTAATTAATGCTGCAATCATTATTATTGTTAATTTAAAAGTCAAAAAACCTAAGTGA", "aa": "MNKNIFNMENGTNLFNATMVLSLMCIPTMINSLLLQFEKVDLCLKLSGSAIGLSESKVIYKIIKKSLNKKIFLIYISSFARVIGESAALMFILNSNNYSENFTNIKKFFNSSLRPLSVFINANFFAENGGDATRSYMFNLAFVLLIVITLINAAIIIIVNLKVKKPK", "aa_hexdigest": "db0b149afdec936373812f7fe8b69273", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18722.1494, "isoelectric_point": 9.791409873962401 }, "id": "IGIGPGHMMC_1470", "locus": "GCA000148625_01464" }, { "type": "cds", "sequence": "contig_1", "start": 836173, "stop": 836478, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCAACTTTAGAGCTTTATTTAACACTTTATATATCTCTTTAGTAGCAATTTTAATAACCTTCCCTTTTGCTTTTTTAGTAACAATTTATTTTGTTGAATTTAACAAAAGCAAAAAAGCAAAATTAGTATTTGATAACTTCATTTCAGTTATGACTTCAATGCCTGCTTTACTTTTTGGTGTCTTTGGTTCAGCTATCTTTTTAGGAACATTTCAATTGGCTTTAGGTGGTAAAACCAGTAACTCACTTTTAGCTGGAGCTTTAGCAATTAGCGTTATGTTATTTGCCTTCAGCTGTCGCTGA", "aa": "MGNFRALFNTLYISLVAILITFPFAFLVTIYFVEFNKSKKAKLVFDNFISVMTSMPALLFGVFGSAIFLGTFQLALGGKTSNSLLAGALAISVMLFAFSCR", "aa_hexdigest": "d563540ff53f872dd99ef91fa7232763", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11025.121799999988, "isoelectric_point": 9.899652290344235 }, "id": "IGIGPGHMMC_1471", "locus": "GCA000148625_01465" }, { "type": "cds", "sequence": "contig_1", "start": 836614, "stop": 836868, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGCTAAAATCTTTTCAGAAACAACACCATTTATTTTAACTAGCGGACTAAGTAATTCAAAACATATTAGCTTATTATTATTTGGCCAAACATTAACAACTAGAATGAATGCTCAATTATCATTACCTTCTGCTTACTCAATTCCAACTATGTACTACTGTGCTATGATGTCATTTGTGACAATAGTTATCCTCTTTTCATTATCAGAATTTGTGATTCCAAAATTAAGCAAATTAAAAAGAAAACATTAG", "aa": "MLAKIFSETTPFILTSGLSNSKHISLLLFGQTLTTRMNAQLSLPSAYSIPTMYYCAMMSFVTIVILFSLSEFVIPKLSKLKRKH", "aa_hexdigest": "4dec9c3a8855e01ce8fb678273f9d50d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9449.254799999995, "isoelectric_point": 9.921055793762207 }, "id": "IGIGPGHMMC_1472", "locus": "GCA000148625_01466" }, { "type": "cds", "sequence": "contig_1", "start": 837036, "stop": 837647, "strand": "+", "frame": 3, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB 3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "nt": "TTGAATCGAATTCATGGGCCACAAGCTGGAAATTATCTAGAAGGTAAAGTTGAATACTTAAATCAAGATCTCTTTAGCAAAAAATTTATTAATGATATTGAATTAAGAAAGCAAGTCGGAATGATATTTCAACAGCCAAGTGTTTTCCCTCTTTCTATTTATGATAATGTTGCTATTGGACTAAGAAATATTGGAATTAGAGATAAAAAAATATTAGACCAAAAAGTGCAAGAAGCTTTAGAAAGAGCTGCACTTTATGATGAAGTTAAAGATAAAATTAAAGAAAATGCAGAAGCATTAAGCGGTGGTCAAAAGCAAAGATTAGCTATTGCTCGAGCAATAGCGCTAAGTCCAAAAGTACTTTTAATGGATGAACCTACTAGTGCGCTAGATCCAATTGCAACTCAAAAAATAGAGAACTTAATTTTAGATTTAAAAAATGATTACACCATTATTTTAGTAACTCACTCAATGGCTCAAGCTCAGAGAGTTAGTGATAAAACTGCATTCTTTTTAGCAGGTGAATTAATTGAAATTAATAGCACTAGAAACATATTTACTAAACCACTTAAAGAAGAAACAAAAGACTACATTATGGGAAAGATAGGATAG", "aa": "MNRIHGPQAGNYLEGKVEYLNQDLFSKKFINDIELRKQVGMIFQQPSVFPLSIYDNVAIGLRNIGIRDKKILDQKVQEALERAALYDEVKDKIKENAEALSGGQKQRLAIARAIALSPKVLLMDEPTSALDPIATQKIENLILDLKNDYTIILVTHSMAQAQRVSDKTAFFLAGELIEINSTRNIFTKPLKEETKDYIMGKIG", "aa_hexdigest": "fe4c4f74239b55db94544137b65ef815", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2N8PY15", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.557, "score": 237.0, "evalue": 8.16e-76, "valid": false, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P0A2V9", "go_ids": [ "GO:0005524", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P0A2V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "product": "Phosphate import ATP-binding protein PstB 3" }, "genes": [ "pstB" ], "id": "IGIGPGHMMC_1473", "locus": "GCA000148625_01467" }, { "type": "cds", "sequence": "contig_1", "start": 837652, "stop": 837870, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATAAATTACACTATATTAAAGAAGAGCGAAGATAACTTAAGAAAAATGTTTTTTGATTACTTAGACCAAGCATATCAAATGCATCTTAAATTAACTGAACTTATTTCAATATATCAAAACAAAATTGATATTGAAGGCTTATTTTTAGAAATCAATAATCGTGAAAAACAAAGCGACTTAACTCAAGTAAATACCATTGAAGAATTGCTTTGA", "aa": "MPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELL", "aa_hexdigest": "60f59d4d414ef04a68b2c46a124400e2", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8668.875899999992, "isoelectric_point": 4.781091499328612 }, "id": "IGIGPGHMMC_1474", "locus": "GCA000148625_01468" }, { "type": "cds", "sequence": "contig_1", "start": 837973, "stop": 838353, "strand": "+", "frame": 1, "gene": "phoU", "product": "PhoU domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41", "UniRef:UniRef50_UPI0038737223", "UniRef:UniRef90_C4XF41" ], "nt": "ATGGCCAGATTCTTCTATCAAAACAAAAAAATTCCTCAAGAGGTTGTTAATGCTGCTTTAGCTATGAATATAGAATTAAGCAAAATTTTTAAAGAATATAGTAGCCGTCTTCAAGATGAAAAAAGTACTAAAGAATTCTTCAAAGAATTCCAAAAACAAAAAGTAGAATACAAACAATTCTGTGATAAAGAGATCAAAAAAATGGCTCGTAAATTAACCAAAAATGATGAGTATTCTAACGAAATAATTATTAAGTTTTCTTTTGAATTGCACTCATATTATCGTGTAGTTGAAAGACTAGAAAATATCATTGAAAATTTGATTTTCATTCATGATACAGATTTCTTTAGAAAGAAATTGGAAAATGAAGAAATGTTTTAA", "aa": "MARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF", "aa_hexdigest": "db0ebe688e8bd3145b04cc565b6aeec5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0038737223", "query_cov": 1.0, "subject_cov": 1.024390243902439, "identity": 0.635, "score": 158.0, "evalue": 9.57e-48, "valid": false, "uniref50_id": "UniRef50_UPI0038737223" }, "pscc": { "uniref50_id": "UniRef50_UPI0038737223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0038737223" ] }, "genes": [ "phoU" ], "truncated": "5-prime", "pseudogene": { "start": 837673, "stop": 838353, "observations": { "mutation": [ 837868, 837910 ], "stop": [ 837868, 837910 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF41", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 1.0, "score": 242.0, "evalue": 2.29e-79, "gene_start": 1, "gene_end": 126, "reference_start": 109, "reference_end": 234, "reference_sequence": "MMPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELLWDISKDQPLLSHLRWYICVLTSSGDIERICDYLYFMARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF" }, "paralog": false, "identity": 0.9911504424778761, "score": 442.0, "evalue": 6.69e-161, "description": "Internal stop codon at 837868, 837910. Nonsense mutation around 837868, 837910.", "psc": { "uniref90_id": "UniRef90_C4XF41", "product": "PhoU domain-containing protein", "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF41", "UniRef:UniRef50_C4XF41" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41" ], "product": "PhoU domain-containing protein" } }, "id": "IGIGPGHMMC_1475", "locus": "GCA000148625_01469" }, { "type": "cds", "sequence": "contig_1", "start": 838562, "stop": 839461, "strand": "+", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707", "UniRef:UniRef90_C4XF40" ], "nt": "ATGTCACTTTTTACAAAATTAGTAAATAAAGCTACTAAAGATGTTTTATTGCAAGCAACTTTTAAAGATGCTAAAAAGATTGAATATTTAATCGAAAAAAACAACGAAATTACAGAATATCTTAGTGAAAACAAAGCTTATGTTTACTTAGGAGAAGAAAGCAAGTTAGATTTTAGAACTCTTGAAAGATTTTTTAGATCTTTAAGTGAAAATAGCGCACGTAACTATTGTGTTGATTTAGCTTCATTTGCAACAAAAAATGTTTCAGTTGCTGATGTTGTTGAAGCTTTTACAAGAGCAGTTTACTTTGTTAAAGCTTCTTTATACAATGAAAAAGCTAAAGATAAAGATGCTAAAGAATTTACTTTAGTTCCTTATGTAGAAAAAGTAACTAAAGAAGTTGAAGAAAGATTAGAAAAAACATTAATTTTAGCTGAAGCTACAAATTATGCTCGTAACTTACAAGTTACACCACCAAACATTTGCAACTCAGAATATTTAGCAGACCAAATTGTTAAAGATTTCAAACAATACAAAAACTTAAAAGTTACTGTTTTAAATAAAAAACAAATTCAAGACCTTAAAATGGGATTATTACTTTCAGTTAACAGAGGGTCAGTTTATGAACCACGTGTAGTTGTTATCGAATATGCTGGTGATCCTTCAAGTAAAGAAAAAACAGTTTTAGTCGGTAAAGGTATTACTTTTGACTCAGGTGGTTACTCACTTAAGAGTCCAAGAAACATGTTAGGTATGAAATTCGACATGTCAGGTAGTGTTATTGTTGCTTCAACATTAAAAGCTATCGCACAACTTAAACCTAAGAAAAATGTTTCAGCAATTATGTGTATTACAGACAACAGAGTTAATGGTGATGCTTCATTACCTGACTCAGTTTGA", "aa": "MSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSV", "aa_hexdigest": "4c7d84cb938ae47eaed40a3780dfca4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 838532, "stop": 839764, "observations": { "mutation": [ 839459, 839636, 839657, 839702 ], "stop": [ 839459, 839636, 839657, 839702 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF40", "query_cov": 1.0, "subject_cov": 0.6402569593147751, "identity": 1.0, "score": 566.0, "evalue": 6.34e-201, "gene_start": 1, "gene_end": 299, "reference_start": 11, "reference_end": 309, "reference_sequence": "MIKLNNRRLIMSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSVWKAMNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGIWATSEKAWEDISKAADNAHELVWRMPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI" }, "paralog": false, "identity": 0.9878048780487805, "score": 772.0, "evalue": 1.44e-284, "description": "Internal stop codon at 839459, 839636, 839657, 839702. Nonsense mutation around 839459, 839636, 839657, 839702.", "psc": { "uniref90_id": "UniRef90_C4XF40", "product": "Cytosol aminopeptidase domain-containing protein", "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF40", "UniRef:UniRef50_P47707" ] }, "pscc": { "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707" ], "product": "Probable cytosol aminopeptidase" } }, "id": "IGIGPGHMMC_1476", "locus": "GCA000148625_01470" }, { "type": "cds", "sequence": "contig_1", "start": 839468, "stop": 839638, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGAATGGTAAAAGCGTTGAAATCAACAACACAGATGCTGAGGGTCGTTTAGTTATGGCTGATGGTTTAGTTTATGGAGCAACCAAACTTCATGCAACACGTTTAATTGACGTTGCTACATTAACTGGTGCTATTCTTATTGCTTTAGGTCAAACCTACACAGGTATTTGA", "aa": "MNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGI", "aa_hexdigest": "6db99d060ca59c84b65830bf83bc4fd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 56, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 8.808778219818421e-24, "score": 80.30740356445312, "start": 1, "stop": 56 } ], "seq_stats": { "molecular_weight": 5832.660399999999, "isoelectric_point": 5.498172187805174 }, "id": "IGIGPGHMMC_1477", "locus": "GCA000148625_01471" }, { "type": "cds", "sequence": "contig_1", "start": 839708, "stop": 839935, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCATTTGATGAAGCATTCTCAAAAGGAATCAAAGCTTCTAAAGTTGCTGATCTTAAAAACACAGACTTAAGCGGTTATGGTGGATCAAGCTCAGCTGCTATGTTCTTAAAAGAATTTACAGAAGGCGTTGAATACATCCACTGTGATGTCGCAGGTACAGCAGACATTAATGAAGTGCCACAAGGTGTTTTAGTCAAAACACTTACAGAGCTTGCTTTAATTTAA", "aa": "MPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI", "aa_hexdigest": "2494b773a88588688a397ca7310615fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.92, "hmm_cov": 0.974025974025974, "evalue": 1.749256892420417e-14, "score": 49.7672004699707, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 7940.935899999997, "isoelectric_point": 4.534353446960449 }, "id": "IGIGPGHMMC_1478", "locus": "GCA000148625_01472" }, { "type": "cds", "sequence": "contig_1", "start": 840008, "stop": 840241, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTAAAACAAAGTGATGGGATTTTAGAAGTTATAACTGGCCCAATGTTCTCAGGTAAAACAGAAGAGCTTATTAAAAGAATTAAAATTTTAGAAATAGCAGATATTAAAACTTTAGTTCTTAAACCTAAATTTGATACTCGCTTTTCTGAAACAGAAATAGTAAGTAGAACAGGTGCAAAAGTTATAGCTAAAAATATTAAAAAATCAAGTGATATAATGAAATTTTGA", "aa": "MYLKQSDGILEVITGPMFSGKTEELIKRIKILEIADIKTLVLKPKFDTRFSETEIVSRTGAKVIAKNIKKSSDIMKF", "aa_hexdigest": "200ea860849e074e752178a5ca9bc592", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8732.321499999995, "isoelectric_point": 9.646098136901855 }, "id": "IGIGPGHMMC_1479", "locus": "GCA000148625_01473" }, { "type": "cds", "sequence": "contig_1", "start": 840359, "stop": 840586, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCTTAAGAAGACCTTTTGGTGTAACTCCTGCAATCTTAGCAATAGCAGATCACATTACAAAGCTAAAAGCGGTATGCATGAAATGTAAATCTAGTGCAGGTTTTAGTTTTAGAAAAACAAACGAACAATCATTAAATTCGCTTGGTGATGATACTTATGAAGCTCGTTGTAGAGTCTGCCACATCAAAGGTGAACTAAAGAAAAAAGAAGACAAAAAATAA", "aa": "MDFLRRPFGVTPAILAIADHITKLKAVCMKCKSSAGFSFRKTNEQSLNSLGDDTYEARCRVCHIKGELKKKEDKK", "aa_hexdigest": "679e689a618fc70ef86a201cca496f56", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8475.851199999996, "isoelectric_point": 9.509747505187988 }, "id": "IGIGPGHMMC_1480", "locus": "GCA000148625_01474" }, { "type": "cds", "sequence": "contig_1", "start": 841218, "stop": 841355, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATTTCACAATTAGATGTTGAAAGTATGAATGATTTAAGAATCAATAAAAACTTCAATACATATAATGGCGCCTTGTATGAAAATGCAATAGCTAATAGTTTATACAAACAGGACTTTAAATTATTTTATTGA", "aa": "MLISQLDVESMNDLRINKNFNTYNGALYENAIANSLYKQDFKLFY", "aa_hexdigest": "e7e701ce5ad9e6acd238915b2cca13f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5323.961100000001, "isoelectric_point": 4.8610639572143555 }, "id": "IGIGPGHMMC_1481", "locus": "GCA000148625_01475" }, { "type": "cds", "sequence": "contig_1", "start": 841467, "stop": 841592, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAAGTTGAAAGAATTTATGATATGGTTGGCAAATTCTTAGGACGTGAAAACAAGAAATTTGTTTTTAATGATCTTAATTTGAATTTAAAAAGCAAACGCTGTATACCTTCTATAGACTGA", "aa": "MMKVERIYDMVGKFLGRENKKFVFNDLNLNLKSKRCIPSID", "aa_hexdigest": "2baba0b95ee0a5e4699a863967c3e6b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8009, "isoelectric_point": 9.65273838043213 }, "id": "IGIGPGHMMC_1482", "locus": "GCA000148625_01476" }, { "type": "cds", "sequence": "contig_1", "start": 841888, "stop": 842166, "strand": "-", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8", "UniRef:UniRef50_UPI000FF29B85", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef90_UPI000FF29B85" ], "nt": "ATGAATGAAATAAAAGAATTGTTTAATAACAAAAATGTTAAATCTTATGAAGTTGATGATTTAATTAAATTTATTTCTTTTGCAAAAAGCGGTTTTGAATTTGTGCCAAATGAAACTTTAATATTTTTTGATGAAATTCAAGAACAACCTAATTGCATTACAAGTTTAAAAAGTTTTGCCTTAGACAAAAGATTTGATGTTATTTGCTCAGGTTTTTTATTAGGAATTAATTTTAAAGAACACAATATTAGACACATTGGAGTCGGTTATATTTCTTAG", "aa": "MNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS", "aa_hexdigest": "c7c822c944341a0033cd2393e79e7013", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF29B85", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 186.0, "evalue": 4.78e-60, "valid": true, "uniref50_id": "UniRef50_UPI000FF29B85" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF29B85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF29B85" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 841888, "stop": 842328, "observations": { "mutation": [ 842292 ], "stop": [ 842292 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP8", "query_cov": 1.0, "subject_cov": 0.6301369863013698, "identity": 1.0, "score": 189.0, "evalue": 3.25e-60, "gene_start": 1, "gene_end": 92, "reference_start": 55, "reference_end": 146, "reference_sequence": "MLKRKIDAFLLDWKNDANKKPLIIKGARQIGKTTSILEFAKNNYKYVVYINFALMNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS" }, "paralog": false, "identity": 0.9931506849315068, "score": 290.0, "evalue": 2.13e-102, "description": "Internal stop codon at 842292. Nonsense mutation around 842292.", "psc": { "uniref90_id": "UniRef90_C4XDP8", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef50_C4XDP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8" ], "product": "AAA domain-containing protein" } }, "id": "IGIGPGHMMC_1483", "locus": "GCA000148625_01477" }, { "type": "cds", "sequence": "contig_1", "start": 842458, "stop": 842553, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGAAGTTAAACATTTTCCTATTGAACCAGAAAGTAAACAAACAATGGCTGAAATCAAAGTTTACTATGATACAAAACTAAAAAGTTAA", "aa": "MKYEVKHFPIEPESKQTMAEIKVYYDTKLKS", "aa_hexdigest": "1de529985a91ef3d671f0a9d10a6f1d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3762.3519000000006, "isoelectric_point": 8.029940223693849 }, "id": "IGIGPGHMMC_1484", "locus": "GCA000148625_01478" }, { "type": "cds", "sequence": "contig_1", "start": 842782, "stop": 842937, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCAAAGTACTATTAGATTCTTAAGACCAAAAGTTAATTTATTATTAGTTGATGGGTTAGTTATCTCAGAAAATACAAGTGATTATTTTACTGATTTAATTTATAAAACAGTTCACAAGAGCTTTCTTGGTGGAGTTGAAAAAGATAAATGA", "aa": "MPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDK", "aa_hexdigest": "2300050d00982d2ac0e47781460d3841", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5827.7034, "isoelectric_point": 8.085769844055175 }, "id": "IGIGPGHMMC_1485", "locus": "GCA000148625_01479" }, { "type": "cds", "sequence": "contig_1", "start": 843142, "stop": 843237, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATGGTTCAGCTTATGTTTATGACGTTAATAAAGGCTTTGTTGATGATCACGCTACTGGAAAAGGCACATCAAAAGATAAGCCTAATTGA", "aa": "MIYGSAYVYDVNKGFVDDHATGKGTSKDKPN", "aa_hexdigest": "261fd1280e2c1c445ec61071177c4d8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3378.677200000001, "isoelectric_point": 6.505472755432128 }, "id": "IGIGPGHMMC_1486", "locus": "GCA000148625_01480" }, { "type": "cds", "sequence": "contig_1", "start": 843265, "stop": 843999, "strand": "-", "frame": 2, "gene": "potD", "product": "PotD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4", "UniRef:UniRef90_Q6GZ00" ], "nt": "ATGAACAACAACAAACCAAAGAACAACAAATTTAAAACACTTATTAAAACCAAAATTTTAACCAAAACTTTTGGTACAAGTGCTCTTGCAGTTGTTGCTGCAGCTGCTTTAGTTGGTGCTTTATCTTATAAATATAGTAACAAAAATAACTTCAAGCCTTCATTCTACAACTATCAATCTTATATTGATCCAAATACCAAAGCAAAAATAAATAAAAACTTCAACTTCAAAGAATTTGCTGAAATTCAAGATTTTACTAAAGCTATTTTAACTAATAAAGCAGCTGCCGGAATTGGCAACGATTCTCAAGCAGTTCAACTTATTAGAGAGGGTAAACTTCGCAAAATTGATTATGCTAAAATGTTTGGTGATCCTAAATTTAATGACCGCAATGAAGTTTTAAAACTTTTAACTGACACAGTAAAAAATCATCTTGAGTCATATAACAAATATTTAACTAAAGATAAATGTATTAATGCCGATGGTTCGCAAGCTACTGAGGATCGTCACTTAATTGACTACTTTGCTCCATACTTTTCACAAGATATGGTAGTAGCTTATAATCCTGCTAAAGTTTTAAATCTTAAAAAAGATGACCCTGAATATCAAAATAAAATCAATGCTCATCAACAAGCAATGCAAAATCACCTAATTAATGAAATAAAAAACAACAAAGGTGAATTTAGACTCATTGATATTCTTAAAACATTAAAAGCTAACAAATATGAACATTGA", "aa": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEH", "aa_hexdigest": "e1bfe170e3e67bb5f4a7d5dea9992b56", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 842962, "stop": 843999, "observations": { "mutation": [ 843144, 843267 ], "stop": [ 843144, 843267 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GZ00", "query_cov": 1.0, "subject_cov": 0.4756335282651072, "identity": 1.0, "score": 476.0, "evalue": 8.75e-166, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEHWEATNAVRDNMIYGSAYVYDVNKGFVDDHATGKGTSKDKPNWYKEHIDHFVELFNHGIGYSVRDTKHINFNGDGQFLLNNLINPDSKTCAGLIYNGDAVDAYFSEDNYATVPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDKWINGPKWNSTTEEWNNIEGYESFTTFDFVGYTPAWKSQYEFVKNNYFVDDKYPQYIASLYGINKEQTIFDVDNTTPKMKYEVKHFPIEPESNQPMAEIKVYYDTKLKS" }, "paralog": false, "identity": 0.991304347826087, "score": 694.0, "evalue": 5.03e-254, "description": "Internal stop codon at 843144, 843267. Nonsense mutation around 843144, 843267.", "psc": { "uniref90_id": "UniRef90_Q6GZ00", "product": "PotD", "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GZ00", "UniRef:UniRef50_A0A3P8K8Q4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4" ], "product": "Hypothetical lipoprotein (MG045 family)" } }, "id": "IGIGPGHMMC_1487", "locus": "GCA000148625_01481" }, { "type": "cds", "sequence": "contig_1", "start": 844115, "stop": 844246, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCATCAATTGTTTTTGCTGGTTTAGTTTCCATTTTCTTATCATTTGATGACTTTATTATTTTACAAACAACTTCAAATGTAAGTACACTTGGAACCAAACTTTATGAAGGTCAATTTAAAGCTTGA", "aa": "MIPSIVFAGLVSIFLSFDDFIILQTTSNVSTLGTKLYEGQFKA", "aa_hexdigest": "583831a1e41804129e9a082136436fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4713.4439, "isoelectric_point": 4.556577491760254 }, "id": "IGIGPGHMMC_1488", "locus": "GCA000148625_01482" }, { "type": "cds", "sequence": "contig_1", "start": 844268, "stop": 844405, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGAGGAATAGTAGGTCACACAATTATGGCTTTACCTTATGGTATTACTTTAATGTATCCAAGAAGTGAAAAATTCAATGCTTCATTATTTGAAGCGAGCCAAGATTTAGGTTATTCAAAAATTAGATCATGA", "aa": "MIRGIVGHTIMALPYGITLMYPRSEKFNASLFEASQDLGYSKIRS", "aa_hexdigest": "5a6f6131de25d47b54e7db228b0d492f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5063.871700000001, "isoelectric_point": 9.396283149719238 }, "id": "IGIGPGHMMC_1489", "locus": "GCA000148625_01483" }, { "type": "cds", "sequence": "contig_1", "start": 844812, "stop": 845243, "strand": "-", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5", "UniRef:UniRef90_C4XDP5" ], "nt": "ATGGTTTTTGGTTTAACTTATTTAAACTTGCCATACATGATTATGCCACTTTACACAGTCTTTAAAGACATGCCTAAAAACATTCTTGAAGCTAGTGCAGACTTAGGTTACAACAAATTTCAAACATTATTTAAAGTAGTTGTTCCATATAGTTTCAAAGCTATTTTAAGTGGTTTCAGTATGATCTTCTTATCATCAGCAACCACCTTTGTTATTAGTGCAAAATTACTTCCAAATGGTACTCAATTACAAACTATTGGATCTGTAATTAATGATTATTCAAGCCCTTCAAACAAATATGAATTAGCTTTAGGAAGCACACTTGTTTTAGTTGTATCAGCCATATTTATAGGTTGTTACGGATTAATCAATTTTGTTCCTAAAATTATAATTAGACTTAAAAAAACTAAAGGAGGTAAGAAACATGACTAA", "aa": "MVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKTKGGKKHD", "aa_hexdigest": "e72b71933408076c9e334f4f24f675a3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 844812, "stop": 845543, "observations": { "mutation": [ 845249, 845465, 845477 ], "stop": [ 845249, 845465, 845477 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP5", "query_cov": 1.0, "subject_cov": 0.5125448028673835, "identity": 1.0, "score": 277.0, "evalue": 2.92e-92, "gene_start": 1, "gene_end": 143, "reference_start": 137, "reference_end": 279, "reference_sequence": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGIWLIIWRSLKIGLISAILCLIIAFPYAYFIATTKSKYLAIYALSLILSPMIIFTIAKIYAIRGFFLSIFEEDSLNAEWFMVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKTKGGKKHD" }, "paralog": false, "identity": 0.9876543209876543, "score": 457.0, "evalue": 7.59e-166, "description": "Internal stop codon at 845249, 845465, 845477. Nonsense mutation around 845249, 845465, 845477.", "psc": { "uniref90_id": "UniRef90_C4XDP5", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP5", "UniRef:UniRef50_C4XDP5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "IGIGPGHMMC_1490", "locus": "GCA000148625_01484" }, { "type": "cds", "sequence": "contig_1", "start": 845475, "stop": 845651, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAAAAATTAAAAACAAACTTGGTTTAAATAAAAGATTACTACTTTTATTACCTTATTTATTTATTGCTATCTTTTTAATAATTTTGCCAATTTTATTAATCGTAATTAATGCTTTAAAACCACAAGATGGCTTTAATGCATCAGCTTTATTAGCTGAAAAAGGAATTTGA", "aa": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGI", "aa_hexdigest": "29f58f503bfeb1c9cc758624beca915a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6428.9259999999995, "isoelectric_point": 10.121810340881346 }, "id": "IGIGPGHMMC_1491", "locus": "GCA000148625_01485" }, { "type": "cds", "sequence": "contig_1", "start": 845707, "stop": 845871, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTATGATGAAAAGAAAAAAGACAAGCTTGTTGGTGAAATTATTAACATGGCTTACCGTGGAAGTTATTACTACTTAAAAATTCAACTTGACAATGATGAAATCATTTATGTTGAAACAGCTAAAAAATTCGAAATAGGTGAAAAAGTAAACTTAAGCTGA", "aa": "MAYDEKKKDKLVGEIINMAYRGSYYYLKIQLDNDEIIYVETAKKFEIGEKVNLS", "aa_hexdigest": "eb4577405dac820bbf002ec5575ec7dc", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.2701, "isoelectric_point": 5.277580070495604 }, "id": "IGIGPGHMMC_1492", "locus": "GCA000148625_01486" }, { "type": "cds", "sequence": "contig_1", "start": 846034, "stop": 846393, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "nt": "ATGGACCAAGAAGTTAAAGAAATTGTAGAATTAGTCGGACTTCAAGGTAATGAAAAAAGATCTATTAATGAACTTTCGGGTGGTATGAAACAACGTGTTGCTTTAGCCAGATCTCTTGTTATTGAACCTGCTATTCTTTTATTAGATGAACCTCTTAGTGCTCTTGATGCCAAAATTAGACAAAAAATGCAAATATTATTAAGAAGCTTACAACAAAAATTAGGTCTTACATTTATTTTTGTTACACATGATCAAGATGAAGCTCTTGAACTTTCAGATAGAATTGCAATTATGCGTGGTGGAAAAATTGAACAATATGACACGCCAAAAAATATTTATGACTATCCAGTTAATAAATGA", "aa": "MDQEVKEIVELVGLQGNEKRSINELSGGMKQRVALARSLVIEPAILLLDEPLSALDAKIRQKMQILLRSLQQKLGLTFIFVTHDQDEALELSDRIAIMRGGKIEQYDTPKNIYDYPVNK", "aa_hexdigest": "65238b594da9ee4225894c7f760cd10d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021D517A5", "query_cov": 0.9663865546218487, "subject_cov": 0.8273381294964028, "identity": 0.53, "score": 123.0, "evalue": 5.73e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021D517A5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021D517A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_1493", "locus": "GCA000148625_01487" }, { "type": "cds", "sequence": "contig_1", "start": 846499, "stop": 846591, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAGATGCTCTACAAGAAAAATATGAAAGAGAAATGGAAACATTAAAACCAAATACTTTTGCATATCGTCGTAGACAAAAATGA", "aa": "MKKLDALQEKYEREMETLKPNTFAYRRRQK", "aa_hexdigest": "84a39694df3e377086249004b9412e74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3802.3878000000013, "isoelectric_point": 9.867998313903808 }, "id": "IGIGPGHMMC_1494", "locus": "GCA000148625_01488" }, { "type": "cds", "sequence": "contig_1", "start": 846613, "stop": 847008, "strand": "-", "frame": 2, "gene": null, "product": "Multiple sugar ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0", "UniRef:UniRef90_A0A3P8MEI0" ], "nt": "ATGATTAGGAAAAATAATACACCTTATATTATTGAATTAAAAGAAGTAGTTAAAGAATTCGATGATAAGGTTGTTTTGGACAATGTTGATTTAAAAATCAATAAAGGTGAATTTGTTACTTTGCTAGGACCTTCAGGATCTGGCAAAACTACAATCCTTAGACTTATTGCTGGTTTTGAAAGAGCAACACGTGGTGAAATTAAATTTCATGGTGTAGATATTAAAGACTTGCCGCCTCACAAAAGAGACCTTTCAACTATTTTTCAAGATTATGCATTATTTCCTCACTTAGATGTTGAAGGAAACATTAAATATGGTTTAGCTCTTAAAAGAAAACCAAAAGAAGTTATTAATGCTAAACATGAAAAATTATTAGTTCAAAAACAAAAACAATGA", "aa": "MIRKNNTPYIIELKEVVKEFDDKVVLDNVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLDVEGNIKYGLALKRKPKEVINAKHEKLLVQKQKQ", "aa_hexdigest": "132d9bd15df8f049e074421499d0a8cf", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 846415, "stop": 847008, "observations": { "mutation": [ 846468, 846501, 846615 ], "stop": [ 846468, 846501, 846615 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEI0", "query_cov": 0.9923664122137404, "subject_cov": 0.6372549019607843, "identity": 0.9540000000000001, "score": 246.0, "evalue": 1.55e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MIKKNNSPYIIELKEVVKEFDDKVVLENVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLNVEGNIKYGLALKRMPKEVINAKHEKLLAQKQKKWEERAKAEMKKLDVLQTKYEREMKTLKKNTFAYRRRQKWLDKSDFNYSYWENYVDQQTENFEKRYLPSLTNQK" }, "paralog": false, "identity": 0.7614213197969543, "score": 285.0, "evalue": 2.93e-99, "description": "Internal stop codon at 846468, 846501, 846615. Nonsense mutation around 846468, 846501, 846615.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEI0", "product": "Multiple sugar ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEI0", "UniRef:UniRef50_A0A3P8MEI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0" ], "product": "Multiple sugar ABC transporter ATP-binding protein" } }, "id": "IGIGPGHMMC_1495", "locus": "GCA000148625_01489" }, { "type": "cds", "sequence": "contig_1", "start": 847129, "stop": 847281, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527148.1", "SO:0001217", "UniParc:UPI0001F59906", "UniRef:UniRef100_A0AB32XDD3", "UniRef:UniRef50_A0A168RBX0", "UniRef:UniRef90_A0AB32XDD3" ], "nt": "ATGAGAATACAAAATTTTAAGCGTGATAAATTTAAATCTAAATTTGTATTATTAAACTTTTGCGAAGATAAAAATCCAGATCTTCCATTTTTTGTTATTGAGTATTCTCAAAACGTGTTTGATTTAGCCTCCCTAAGAAGTCAATCGATTTAA", "aa": "MRIQNFKRDKFKSKFVLLNFCEDKNPDLPFFVIEYSQNVFDLASLRSQSI", "aa_hexdigest": "ca5310ae2eb824651c99281c974007b1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59906", "ncbi_nrp_id": "WP_013527148.1", "uniref100_id": "UniRef100_A0AB32XDD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59906", "RefSeq:WP_013527148.1", "UniRef:UniRef100_A0AB32XDD3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDD3", "uniref90_id": "UniRef90_A0AB32XDD3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDD3", "uniref50_id": "UniRef50_A0A168RBX0" }, "pscc": { "uniref50_id": "UniRef50_A0A168RBX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RBX0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6017.886799999999, "isoelectric_point": 8.977689933776855 }, "id": "IGIGPGHMMC_1496", "locus": "GCA000148625_01490" }, { "type": "cds", "sequence": "contig_1", "start": 848134, "stop": 848409, "strand": "+", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013527151.1", "SO:0001217", "UniParc:UPI0001F59909", "UniRef:UniRef100_C4XFY2", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_A0A3P8LHZ8" ], "nt": "ATGGCAAATATAAAATCAAAAGTAAAAAGCATTGCTAAGATGGAAGAATTTCGTGTACGTAACAATGCTATGAAAACAAGAGTTCGTAAAGCAATTAGAGTAGCTAGAGAAGCAATTTTAGCTAAAGATAAAAACGCTCAAGAGTTAGTTAATAAAGCTCACTCAATTATTGCTACAGCTGTTCAAAAAGGTGTTTTCCACCCAAATAAAGGTGCAAGAAAATCATCTCGTTTAGACAAATTTGTTAACGAACAAAATGCTAAAAAAGATGCATAA", "aa": "MANIKSKVKSIAKMEEFRVRNNAMKTRVRKAIRVAREAILAKDKNAQELVNKAHSIIATAVQKGVFHPNKGARKSSRLDKFVNEQNAKKDA", "aa_hexdigest": "523ee6cf391377419fa46df72ea79c4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59909", "ncbi_nrp_id": "WP_013527151.1", "uniref100_id": "UniRef100_C4XFY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59909", "RefSeq:WP_013527151.1", "UniRef:UniRef100_C4XFY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY2", "uniref90_id": "UniRef90_A0A3P8LHZ8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LHZ8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "IGIGPGHMMC_1497", "locus": "GCA000148625_01491" }, { "type": "cds", "sequence": "contig_1", "start": 848471, "stop": 848614, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTTAGCACTTCAAAGAAAATTGACTCTTCCTGAATGTGCTTTAACTGACGTCTTCTTCTTGCCTCACTTTAACAAACCATTTAATTTCTTCTTAATGCCAATGCTTAAAGCACTTGGACTTAAATATAAAGCTTAA", "aa": "MFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA", "aa_hexdigest": "b0f21f40ec855f6bdf05dc44c1ed6776", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5462.648099999999, "isoelectric_point": 9.746475410461425 }, "id": "IGIGPGHMMC_1498", "locus": "GCA000148625_01492" }, { "type": "cds", "sequence": "contig_1", "start": 848645, "stop": 849244, "strand": "-", "frame": 1, "gene": null, "product": "NADH oxidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1", "UniRef:UniRef90_C4XFY1" ], "nt": "ATGCAAAAAAACATGAAAAAAGAAGGTGTTAAACTTGCTCTTGGAGAAAAAGTTGTTGAATTCAAATCAAAAGATGGAAAACATGTATCACAAGTTATTACAGATAAAAGTAAATATGATGCAGATCTTGTAATTATGTCAGTAGGTTTCAAACCTAGAACATATGTATTGAATGATGTTGAAAAATTACCTAATGGAGCAATTAAAGTTAATGAATTCCAACAATCAATTTCTGACGAAAACATCTATGTTTTAGGTGATTCTGCAGCATTAAAACACTGTGTAAGCAACACATATGAACACGTAGCTTTAGCAACTAATGCAGTTAAAACAGGTATTGTAGCTGCATTAAATTTAGCTGGACTTAAAGTGCCATTTCCAGGAGTAGTAGGAACAAATGCTATTAATGTTTTTGGTTGTCACTATGCTGCAACAGGATTTAATGAAGATGCAGCTAGAGCTCATGGATTTGATGTAGCTAGTGAATATTTTGAAGACAATGACCGCTGCGAATTTGCTGGTAAAAGTGAAAAAGTTGGATGCAAAATTATTTTTGACAAGAAAACATTACAAATTTTAGGTGTTCAAATCGGATCATGA", "aa": "MQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGS", "aa_hexdigest": "f32dd76e9dbb953bbcff6925a03c8c50", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 848471, "stop": 849544, "observations": { "mutation": [ 848647, 849493 ], "stop": [ 848647, 849493 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY1", "query_cov": 1.0, "subject_cov": 0.4216101694915254, "identity": 1.0, "score": 389.0, "evalue": 1.02e-132, "gene_start": 1, "gene_end": 199, "reference_start": 216, "reference_end": 414, "reference_sequence": "MNKIKMYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAIWVGGKAKQPDGLFYSSPNILQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGTWPIEPKIPGIELDNILLSKLYQHAQVLVKKANDKKIQNVVVVGAGYIGIELVEAFHNKGKKVTLIDLADRVVPGYLDGEFTDIMQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGSWGQVNHTEVIYMFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA" }, "paralog": false, "identity": 0.988795518207283, "score": 700.0, "evalue": 9.88e-258, "description": "Internal stop codon at 848647, 849493. Nonsense mutation around 848647, 849493.", "psc": { "uniref90_id": "UniRef90_C4XFY1", "product": "NADH oxidase", "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY1", "UniRef:UniRef50_Q4A5P1" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1" ], "product": "NADH oxidase" } }, "id": "IGIGPGHMMC_1499", "locus": "GCA000148625_01493" }, { "type": "cds", "sequence": "contig_1", "start": 849491, "stop": 849640, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAAGAATATGGAGTAAATTTAAAAACTTCACATGAAGTTATCGAAATTGATAGAAAGAAAAAAGAAGTTACAGTTCAAGATTTAGATAGTGGTAAAACCTTTAAAGACACATATGATAAATTGGTTTTTGCAGGTGGTACATGA", "aa": "MQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGT", "aa_hexdigest": "41c581299c451c79791b6a3c1ee58e33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5591.2629000000015, "isoelectric_point": 6.544634819030762 }, "id": "IGIGPGHMMC_1500", "locus": "GCA000148625_01494" }, { "type": "cds", "sequence": "contig_1", "start": 849695, "stop": 849874, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAAATACAAATTTTTATTACAGAAAAGAGGAAATATGAAAATTATTGTCATTGGTGCAAACCATGCAGGGACCAGCTTTTTAAGAACATTAAAAACTGTAAATCCTAAAGCAGAAATTACAGCTTATGACCGTAATACAAACACATCATTTTTAGGTTGTGGTATTGCAATTTGA", "aa": "MYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAI", "aa_hexdigest": "0f86ef6916333231590c079f92902330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6593.6998, "isoelectric_point": 10.104017066955567 }, "id": "IGIGPGHMMC_1501", "locus": "GCA000148625_01495" }, { "type": "cds", "sequence": "contig_1", "start": 850098, "stop": 850649, "strand": "-", "frame": 3, "gene": null, "product": "EVE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0", "UniRef:UniRef90_C4XFY0" ], "nt": "ATGAAAAAACGTAGTCAATCATCATATGTTGACCTTAACGCTATCAAAGCATCTCTTGAACTTAAAATTAAAGAACTTGAAGACCAAAAAAATGCTTCAGATGAAACAATCAATAGCTTAAAAGAACAATTAGAAAACAAAAAATTCGACTTCATGAAAGTTGAAGAAGAAATTGAAGCTATTAAAAACCAAAAAGATCAAGAAATCAACGCAGTACGTGAAGAAGCTGAAAAGAAAATTGAAGAAGTTAAAGCAGAAGCAGCTGCAACAGCAGAACAACCAGCTTATGATCCAAATGCTGAAGTTCAATACGATCCATATGGAAACCCAATTGATCTAAATGCACAGCCAGCAATGGACCCTTATGGAAATCCATATGTACAAAGTGAAACAATTATGGCAGATCCTTATGGAACAGAAGGCGGATATTTAGTACAACCTGAAGCCTATGGGCAATTAACTCCATATGAAGCTAACTATGGATACCCAACAGGTATGTATGATGGTACTCAATTCTACCCTGTTCAACGAAAATCAGACAAAGGAATTTGA", "aa": "MKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGI", "aa_hexdigest": "90f9bd790fb7a7f1256243aa94c1a3e4", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 850002, "stop": 850949, "observations": { "mutation": [ 850100, 850922 ], "stop": [ 850100, 850922 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY0", "query_cov": 1.0, "subject_cov": 0.5111731843575419, "identity": 1.0, "score": 265.0, "evalue": 6.4e-86, "gene_start": 1, "gene_end": 183, "reference_start": 144, "reference_end": 326, "reference_sequence": "MMKRLNLLWKPKEDETYPWLLKHPKIKEGLAKFKTRQDAVDWFLNFGDEVYIWFQNNEGIFGGQLAVLESENPKDKDSKKLLSIPKVSGFDGGETYNGICEEFSIHPSYLSREPYKSALAKRIPEIDFVLISDPATYFPADLEMKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGIWVGVGIAAAVLLIILILDILVLLSLTNVAQIF" }, "paralog": false, "identity": 0.9809523809523809, "score": 513.0, "evalue": 5.32e-186, "description": "Internal stop codon at 850100, 850922. Nonsense mutation around 850100, 850922.", "psc": { "uniref90_id": "UniRef90_C4XFY0", "product": "EVE domain-containing protein", "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY0", "UniRef:UniRef50_C4XFY0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0" ], "product": "EVE domain-containing protein" } }, "id": "IGIGPGHMMC_1502", "locus": "GCA000148625_01496" }, { "type": "cds", "sequence": "contig_1", "start": 851234, "stop": 851719, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGACAGAAAAAAACCAAAACGTCATTTTTATAGATGAATTAGTAAAAAAAGTTAATTTAAATGAAATCAAATTTTTCTTTTTAGAAAAGAGTACTAAACATCAAATAACAATTGAAAAGGAAATTTTAAATCAAAAGAAAATTAGTCATAATTTCGATTTCATATTAAATACTTATAATAAAATAAATAAATTATTAGATGAATCAAAAAACAATAAATGTTATTGTAACAAAATGAATCAAAACAATTTTTTAAGCAAACTTATTTTAAGGCTTGATTATTTTCCTTATGTTTTAAGAAAAGTAATCAAAACTTTAAATCCTTTTTACTTAACAAAATATCTAAAAGAAATGTGTTATTTGATAAATCAAAATCTAAAAGATAAACTTAAAAAAGATTATAGAAACTTCTATCCAATTTTAGAAGCTGTCAAAAATGTATTTGATAATGGACTAAATTTACTTGGAATAGATTTTAAAAATTAA", "aa": "MTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN", "aa_hexdigest": "519a6e33dd9a44f05299dbec9cc1006f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 109, "aa_cov": 0.6273291925465838, "hmm_cov": 1.0, "evalue": 2.867166821429766e-09, "score": 33.0975456237793, "start": 59, "stop": 159 } ], "seq_stats": { "molecular_weight": 19449.599899999997, "isoelectric_point": 9.47048625946045 }, "id": "IGIGPGHMMC_1503", "locus": "GCA000148625_01497" }, { "type": "cds", "sequence": "contig_1", "start": 851864, "stop": 852865, "strand": "-", "frame": 1, "gene": null, "product": "arginine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.19", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_A0A3P8KM35", "UniRef:UniRef90_C4XFX9" ], "nt": "ATGGTTATAGTTCATAAATTGAACAAGTTAATTAAAAACATAGTAAATGAACTAAAAGCTGAAAACAAGATTTTCAGTTCTTTTAAGTTTCTTGATGGTGAAAAGAAGTTTTCACTAAAAATGGCTCGTAAAACTAAGGGCCATAACAATAATATTAACCATATTGAATGCGATATTGTTTTTCAACTTAAAAAATTTAGTTTTATTCCTTATATTGAATTAGCAGAAGAAATTAAAAAGAGATTAATCAAAGATTCAATGGTTAAAGATGTGACAATTGATCGTTATGGGCTTTTACATATTGTTTTGAATGAAAAAGATTTAGTTTCAGTGGTTAATGAAGTTATTAAGAAAGCTTATAAATATGGACAAGAAAAGAAAAATAATATTAAAGTTAATATTGAATATACTTCAGCTTCGCCACTTAATTCTTTAACTTTGATGAATGCTAGAAATGCTTTAGTTGGAGAAACTATAGCAAGAATTTTAGAATACTTAGGTTATCAAGTTACTCGAGAATTTTTAGTTTATCCAGGTTTAAAGAAAATGGAAAAAGTAAGCCAAATTGTTCATGAACAATATGTTAACTTGTTTGAATTAAATGACAATTCTATTAAAGTTGACAAGGATATTTTAGAATGTGCAAAGAATATTAAAGCACAAAATGGTGATCTTTATTTAGACATAAATGACACAATTAAAAGAAATAAATTTGATTTAGCAATTGTTTTTTATTTCTTAAATAAATCCAAAAAACAACTTAAAAATTTAGGTGTCTTTTTTGACATAGTGAGCTATTTTGAAGACATTTTGGTCAAAGAAAAGATTGACAAAGTATTAGAATTATTGTCACCTTTTATTTATGATAATGATTCAAATTTAATACTTAATACAAATCTTGATGATAGTAAAGAACGCATTCTTGTTCATAGAAATGGCACGAGTTCATATTTATTATTAGACATAATTTATCATCTTGAAAAATTAAAAAGAGCAGACTGA", "aa": "MVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRAD", "aa_hexdigest": "5e548d637e71a0b64bdabac03ce65b5f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 851561, "stop": 852895, "observations": { "mutation": [ 851866 ], "stop": [ 851866 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX9", "query_cov": 1.0, "subject_cov": 0.6021699819168174, "identity": 1.0, "score": 641.0, "evalue": 1.15e-228, "gene_start": 1, "gene_end": 333, "reference_start": 11, "reference_end": 343, "reference_sequence": "MYLKIYSGSNMVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRADWIIDILDANHTQYSHSLISALKFLNAYNNNIDIAFCENFQIKENNEVKSMTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN" }, "paralog": false, "identity": 0.9954954954954955, "score": 865.0, "evalue": 3.95e-319, "description": "Internal stop codon at 851866. Nonsense mutation around 851866.", "psc": { "uniref90_id": "UniRef90_C4XFX9", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_A0A3P8KM35", "kegg_orthology_id": "K01887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX9", "EC:6.1.1.19", "UniRef:UniRef50_A0A3P8KM35", "KEGG:K01887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM35" ], "product": "arginine--tRNA ligase" } }, "id": "IGIGPGHMMC_1504", "locus": "GCA000148625_01498" }, { "type": "cds", "sequence": "contig_1", "start": 852940, "stop": 853305, "strand": "+", "frame": 1, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGTTAAAAATTTATGTCTGCGGTCCAACTGTTTATAATGATCTACATATTGGAAATATGGTTCCAATATTAACTTTTGATTTAATGCTAAAAGCTAGACGAGTTTTAGGAGAAAAATTCACTTTTGTTCACAATATTACTGATATTGATGACAAAATAATCAATCGTGCTAGAGAGCTAAATATAAGCGAAAAAGAATTAAGTCAAAAATATACTAAAGACTACTTGAAATTATTAAAAACCTTGGATGTTGACACTATAAGTCATCTTGAAAAAGTTACTGACAATATGGGCTTAATTATTGAATATGTTAATAAGCAAGTTAAAAATAATAATGCTTATATTGACCAAGAAGGTAATGTTTGA", "aa": "MLKIYVCGPTVYNDLHIGNMVPILTFDLMLKARRVLGEKFTFVHNITDIDDKIINRARELNISEKELSQKYTKDYLKLLKTLDVDTISHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNV", "aa_hexdigest": "37afea71354c967a9ddd4b884bac9645", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.9421487603305785, "subject_cov": 0.8571428571428571, "identity": 0.5, "score": 123.0, "evalue": 5.18e-34, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "IGIGPGHMMC_1505", "locus": "GCA000148625_01499" }, { "type": "cds", "sequence": "contig_1", "start": 853669, "stop": 854097, "strand": "+", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC", "UniRef:UniRef90_UPI000F036BAC" ], "nt": "ATGTCAAAATCTTTAGGCAATGTTATTCTTGCAAAAGACTTTTTAAAAGAACATGATCGCGAAATTTTGAAGTTGATTATTTTGAGTTCAAAAATTTCAGCTCCAATTAATGTGACAAATGAATTAATTGAAAATATGTATGTTATTTTGAATAAATACAAAAAATTAATTTTTAAATTCTTTATTAATTTTAAAAATATTGACAAATCAAAAATTAATAAAAATGAAGAATTTATTAAGATAATGAATTCTTTAAAAATAGGCGATTTTTCTGATTTTAATTATTTATTAAATGAACAAATTAAAGAATTCAATAAAACCAAAAATATAAAAAATGCAGAAATAATTTTTAATACTTTAAAAGTTATCCACCCTCAATTAACAATTAAAGCTAACTATAACAAAGCTTTCGAGCTTTTTGACCAATGA", "aa": "MSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQ", "aa_hexdigest": "09abe47dc6b1cdc56047565e701605be", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 853369, "stop": 854175, "observations": { "mutation": [ 853420, 853480, 853630, 854095 ], "stop": [ 853420, 853480, 853630, 854095 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F036BAC", "query_cov": 1.0, "subject_cov": 0.4369230769230769, "identity": 1.0, "score": 272.0, "evalue": 1.17e-89, "gene_start": 1, "gene_end": 142, "reference_start": 158, "reference_end": 299, "reference_sequence": "LSHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNVWFDVKKFENHYGQVANLNLDKMIFEEQNNNKKFEADFALWKKTNVGIQFDSPFSKGRPGWHTECAVLINKHFKNKGLDIHGGGMDLTFPHHENENIQHFALYNQDITKEWIRCGQINYEGEKMSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQWRCFIDKKEYKQADKIRKKLMKLGVY" }, "paralog": false, "identity": 0.9813432835820896, "score": 526.0, "evalue": 2.19e-192, "description": "Internal stop codon at 853420, 853480, 853630, 854095. Nonsense mutation around 853420, 853480, 853630, 854095.", "psc": { "uniref90_id": "UniRef90_UPI000F036BAC", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F036BAC", "UniRef:UniRef50_UPI000F036BAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC" ], "product": "class I tRNA ligase family protein" } }, "id": "IGIGPGHMMC_1506", "locus": "GCA000148625_01500" }, { "type": "cds", "sequence": "contig_1", "start": 854221, "stop": 854688, "strand": "+", "frame": 1, "gene": null, "product": "RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "nt": "ATGGAAAAATTATATGTGTGTGGTAGAAATTCAGTTGAAGATGCTTTAAAAGTAAACTTGCCAATTGAAACAATTTATGTCAACTCATTTCAACTCGCACAAAAATTAAAACAAATTACCAAAGCAAAAATAGAAATGAAGGATAATTCATTTTTTGCTAACTATCGTAATGATAACCATCAAGGTGTCATTGCTATTTTAAGAGACTTCCCTATTTATGATCTTGAAACAATTAAAAAAGATAATCCTGAAAATGTTTTAGTTTTAGACCACATTCAAGACCCTCATAATTTAGGTGCAATCATTAGAACTGCTAATACTTGCGGAATTAAGCATCTTATTATTAGTAAAGAAAGATCAGCTAATATTACTTCAACAGTTTTAAAAGTTAGCTCAGGTGGTTTTGTTAATATGAAAATAATTAAAGTTAATAGTATTAGTGCTACCTTAACAAAACTTAAAAAATGA", "aa": "MEKLYVCGRNSVEDALKVNLPIETIYVNSFQLAQKLKQITKAKIEMKDNSFFANYRNDNHQGVIAILRDFPIYDLETIKKDNPENVLVLDHIQDPHNLGAIIRTANTCGIKHLIISKERSANITSTVLKVSSGGFVNMKIIKVNSISATLTKLKK", "aa_hexdigest": "441e77b1e4b25f25cf0d3eb7a60482b8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0011424F0F", "query_cov": 1.0129032258064516, "subject_cov": 0.8440860215053764, "identity": 0.535, "score": 157.0, "evalue": 2.9e-46, "valid": false, "product": "RNA methyltransferase", "uniref50_id": "UniRef50_UPI0011424F0F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011424F0F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "product": "RNA methyltransferase" }, "genes": [], "id": "IGIGPGHMMC_1507", "locus": "GCA000148625_01501" }, { "type": "cds", "sequence": "contig_1", "start": 854918, "stop": 855511, "strand": "+", "frame": 2, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "RefSeq:WP_013354970.1", "SO:0001217", "UniParc:UPI0001E33011", "UniRef:UniRef100_A0AB32XDA1", "UniRef:UniRef50_C4XFX6", "UniRef:UniRef90_C4XFX6" ], "nt": "ATGAAATATTTACAAAAATTTTTAGACTTACATCAAACACCCACGCAAGAGTTTGATGAATTTTTACATAGCTTAAAAGACAATCAATTGATTATGATATTGGATTATTTTTACAAAAATGAATTTATCAAAAATATCAAAAGCACTTTAATTAGATTTCCTTATATTCCATTAGAAGCTGAAGATATTTATATTGAATTTTTGCAAACCTATTTAGAAGAAGTTAAAAAGTATAATTCAACTGACAAAAATGTTAAATTTTTAAATTTCTTTTTAAATATCTCCAAATTTTACACATTAAATAAAATTAGATATTGGCTTAGAAAAAAAAGAATTCATAACTCACTTATGACTTCAACTGATGAATTGTTATATGTTTTAGATGAGCATTCTGAAGAACAAATTGAACAAAGAATTAATCAAATTGATACTGAAAACTTTTATCATTTATTAACTGACAAAGATAAAAATATAATTAAAATATTGCAAAATAGTATCAATCAAAAGGACAAATTGATTACACCTAGTAAACTAAAAGAATTTAAAACTAAATTTTTAACTAAATTTAATAATTATTTTCATTTTGCCCATTAA", "aa": "MKYLQKFLDLHQTPTQEFDEFLHSLKDNQLIMILDYFYKNEFIKNIKSTLIRFPYIPLEAEDIYIEFLQTYLEEVKKYNSTDKNVKFLNFFLNISKFYTLNKIRYWLRKKRIHNSLMTSTDELLYVLDEHSEEQIEQRINQIDTENFYHLLTDKDKNIIKILQNSINQKDKLITPSKLKEFKTKFLTKFNNYFHFAH", "aa_hexdigest": "28292666f9e5d3f0def93393b654e5de", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E33011", "ncbi_nrp_id": "WP_013354970.1", "uniref100_id": "UniRef100_A0AB32XDA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33011", "RefSeq:WP_013354970.1", "UniRef:UniRef100_A0AB32XDA1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDA1", "uniref90_id": "UniRef90_C4XFX6" }, "psc": { "uniref90_id": "UniRef90_C4XFX6", "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFX6" }, "pscc": { "uniref50_id": "UniRef50_C4XFX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX6" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "IGIGPGHMMC_1508", "locus": "GCA000148625_01502" }, { "type": "cds", "sequence": "contig_1", "start": 855998, "stop": 856249, "strand": "+", "frame": 2, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_A0A4Y6I615", "UniRef:UniRef90_C4XFX3" ], "nt": "ATGATTAGTACAGTTTCTGGAAAAGAAGATAAGGTTGTTGAAGCTTTAAAAAATAGAATTATTAGTGAACAAGTTGAAGATTGCTTTAATCATGAAGCAACACCTGAAGGCGCTTTTAAAATTTTCAAAAAACCAACCTTAACACCTAAAGAATTAGAAAAACAAAAAAATGGTGAAGAATACAAAGTTAAATATGTAAACATGTATTCAGGCTACATTTTTATTAATATGGACATGACAGATAGAGCTTGA", "aa": "MISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRA", "aa_hexdigest": "ed73095dc91506e0802b5751155f2adc", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "both", "pseudogene": { "start": 855962, "stop": 856552, "observations": { "mutation": [ 855992, 856247 ], "stop": [ 855992, 856247 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX3", "query_cov": 1.0, "subject_cov": 0.40487804878048783, "identity": 1.0, "score": 168.0, "evalue": 1.66e-51, "gene_start": 1, "gene_end": 83, "reference_start": 13, "reference_end": 95, "reference_sequence": "MEDIMDKNFQWYMISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRAWFIVRNTQYVTGLIGSSGRGAKPTPVSQHQIDKSFKKEIKLQEDFDKGVTHTKFQIGEIVEIIDGPFKGEQGRVLECSEANKKVTVEIEYLGKKTPADFEYKEIKSQEYE" }, "paralog": false, "identity": 0.9846938775510204, "score": 388.0, "evalue": 1.32e-140, "description": "Internal stop codon at 855992, 856247. Nonsense mutation around 855992, 856247.", "psc": { "uniref90_id": "UniRef90_C4XFX3", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_A0A4Y6I615", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX3", "UniRef:UniRef50_A0A4Y6I615", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I615", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I615" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "IGIGPGHMMC_1509", "locus": "GCA000148625_01503" }, { "type": "cds", "sequence": "contig_1", "start": 856724, "stop": 857383, "strand": "-", "frame": 1, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_D3VPP6", "UniRef:UniRef90_C4XFX2" ], "nt": "ATGAGCTATAGAAAAATTACTAAAAAAGATTTACTTGAAAAATATTCTTCAAAAATTAGCATATTAAAAGAATATGCTCAAATGATTTATGATCAAAATCAAGTTATGAATATCACAGGTTTTAAAACACTAGATGATATTTTTGAACAAGGTATACTTGATTCTATTTTAGCTTTTGAAAATTACATGATGCATTATGATGAAAATCTAGAAAACAAAAAAATATTAGATGTAGGTGCCGGTGCAGGATTTCCTTCAATTCCAATTCTAATTGCATTAGATAATAAGTTCAATTTAACTATTATTGAATCTCAAAAGAAAAGATGCAATTTTTTAGAAAAAGTTAAAGCACGATTTAATTTAAGTGTCAATATAATTAATTCAAGAGTTGAAGAGTTTTTTAAATTAACAAATTACTTTGAGATTATTACTGCTCGAGCTGTTGCAAGCATTAAAGTTTTATATTTAAGTGCAGCACACTTATTAAAAAACAGAGGAAAATTTGTTTTATTAAAAGGCGAAAACTTTAGAGATGAGCTTACTGATATTGTAAATATTGCTAAATACATGGGCGAAATGGGCGGCTTTGAATATGATAATGTTTTAAATGAAACGTCTAGCGTGGTGGTTATTAACAAAGGAAAAATTCCTGATGATTGA", "aa": "MSYRKITKKDLLEKYSSKISILKEYAQMIYDQNQVMNITGFKTLDDIFEQGILDSILAFENYMMHYDENLENKKILDVGAGAGFPSIPILIALDNKFNLTIIESQKKRCNFLEKVKARFNLSVNIINSRVEEFFKLTNYFEIITARAVASIKVLYLSAAHLLKNRGKFVLLKGENFRDELTDIVNIAKYMGEMGGFEYDNVLNETSSVVVINKGKIPDD", "aa_hexdigest": "8aa655d6e513edef9385f9978c75a92d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX2", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 1.0, "score": 425.0, "evalue": 4.05e-150, "valid": true, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_D3VPP6", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPP6" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "IGIGPGHMMC_1510", "locus": "GCA000148625_01504" }, { "type": "cds", "sequence": "contig_1", "start": 857383, "stop": 858363, "strand": "-", "frame": 2, "gene": null, "product": "Ribose-phosphate diphosphokinase", "db_xrefs": [ "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_013354975.1", "SO:0001217", "UniParc:UPI0001E33016", "UniRef:UniRef100_A0AB32XD72", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_A0A3P8K8X3" ], "nt": "ATGGATAAAAAAAATGTAATGCTTTTTGGTATGCCGAATAGCTTGAAATTAGCAGAAAAAATTTCAAAAATTATTAAAGTTCCTTTGACAGGAATTACTAAAACAACTTTTGCTGATGGCGAAGTTATGTTAGTTAGCGACAAAACAGTTAGAGAAAAAGATGTTTTCGTTATTGCTTCAACATCAAGACCAGTTAATGAAAACTTAATGGAGTTATTCTTATTTATTGACTCGCTTAAAAGAGCAAGTGTTAAAAGTGTTACAGTTGCTTTAAGTTACTATGGATATGCACGTCAAGATCGCAAAGCAAGTGGACGTCAACCTATTGGTGCAAAACTAGTAGCAGACTTATTACAAACAGCGGGTGCTGACAAAGTTATTGCAGTTGATTTACATAACCCTGCAATTCAAGGTTTCTTCAATATTCCTATTGATGATTTGCGTGGTGCTTATACTTTAGCAAAAGCTATTAAAAATACACATGAATCATTTACTGTTGTTTCACCTGACCACGGTGGAACAATTAGAGCAAGAAAACTTGCTGAATTAATTTCAAACGATGTAAAAATTTGTATTATTGACAAACGTCGTATTGGCACAAACCAAACTGAAGTTTTAGGACTTATTGGTGATATTGAAGGTCAAAATGCAGTTATCATTGATGATATCATTGATACTGGAGGAACAATTTTAAAAGCAGTTGATACATTAAAAGCACACGGTGCAAAGAAAATTATTGTAGCTGCAACTCACGGTATTTTTACAAAAGGATTTGAAATTTTTGAAAATAATCCAAATGTTTATAAAGTAATTATTACTGATTCAATTGAAAATGCAGAATTAAGAGATGGTAAATTCAAAAAACTCGAAGTAATAAGTCTTGACAGATTCCTAGCTAATGTTATTGAATCATCAATTAGTGGTAAATCTATTTCTCATTTATATCAACAATTTGCAAAAGAAATTACTTCAAATAAATAA", "aa": "MDKKNVMLFGMPNSLKLAEKISKIIKVPLTGITKTTFADGEVMLVSDKTVREKDVFVIASTSRPVNENLMELFLFIDSLKRASVKSVTVALSYYGYARQDRKASGRQPIGAKLVADLLQTAGADKVIAVDLHNPAIQGFFNIPIDDLRGAYTLAKAIKNTHESFTVVSPDHGGTIRARKLAELISNDVKICIIDKRRIGTNQTEVLGLIGDIEGQNAVIIDDIIDTGGTILKAVDTLKAHGAKKIIVAATHGIFTKGFEIFENNPNVYKVIITDSIENAELRDGKFKKLEVISLDRFLANVIESSISGKSISHLYQQFAKEITSNK", "aa_hexdigest": "8bda781252f5dfa72b5917a58f1def57", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI0001E33016", "ncbi_nrp_id": "WP_013354975.1", "uniref100_id": "UniRef100_A0AB32XD72", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33016", "RefSeq:WP_013354975.1", "UniRef:UniRef100_A0AB32XD72" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD72", "uniref90_id": "UniRef90_A0A3P8K8X3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8X3", "product": "Ribose-phosphate diphosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [], "id": "IGIGPGHMMC_1511", "locus": "GCA000148625_01505" }, { "type": "cds", "sequence": "contig_1", "start": 858544, "stop": 859659, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_C4XFX0" ], "nt": "ATGCTTTTTAAAAATGAATATTTTTATATAATTATAAACATGGAAAAAAATATGTTCAATTCGTTGTCTGATATTAAAAATACCTATGACAATTTAGTTAAAAAATTGGATGATCATGAAGTTATCAATAACATCAAGGAATACACAAAAATAAGTAAAGAAGTCAACAAAATTAAGGATATAGCAGAAACCTTTACTAAGTACTTAAATTTACAAAATGACTACAATGATGCAAAAGTAATGATCAATTCAAAAAATGAAGAGGAAGTTCTTTTTGCTAAAAGTATCATTGAAGAAAATGAAGTTAAATTAGCACAACTTGAAGAAGAATTAAAAATTTTAATTCTGCCTAAAGATGAAAACGATGACAAGAATGTTATTATGGAAATTAGAGGTGCAGCAGGCGGTGACGAAGCTAATATTTTTGCTGGCGACTTATTCAAAATGTACACAAAATTTGCAGAAGATTTAAATTTCAAATTAAAAGTTATATCTTCTTCAGCAGCTAGTGTTGGTGGTTTTACTCAAATAGTATTTAGTATTAAGGGTGATAAAGCTTATTCAAAACTTAAGTTTGAAAGTGGAGTGCATCGTGTGCAACGCGTACCTGTAACTGAAAGTGCAGGTAGAATTCATACTTCAACAGCAACAGTTACTGTTATGCCTGAAATTGATGATAGTGTTGATATTGAAATCAAACCACAAGATTTACAAATTGATACATATAGATCATCTGGAGCTGGTGGCCAATCAGTTAATACAACAGACTCAGCGGTTAGAATTACACACTTGCCTACTGGTATAGTTGTTTCATCGCAAGATGAAAGAAGTCAAATAGCAAATAGAGAAACTGCTTTAACAGTTCTTAAAAGTAAGCTTTATGATTTAGAAATTCAAAAGAAACAACAAGAAGAAGCAGGGTATAGAAAACTAGCAGGTCATGGTGATCGGAGCGAAAAAATTAGAACATATAACTATCCACAAGATAGAGTTACAGACCATAGAATTGGATATTCAACAAGTTTAAAACAAGTAATGGAAGGTAAATTATTAACTATTATTGAAGCATTACTAACAGAAGAACAAAATCAAAAAATAAAGGAAAGTGGCTTATAA", "aa": "MLFKNEYFYIIINMEKNMFNSLSDIKNTYDNLVKKLDDHEVINNIKEYTKISKEVNKIKDIAETFTKYLNLQNDYNDAKVMINSKNEEEVLFAKSIIEENEVKLAQLEEELKILILPKDENDDKNVIMEIRGAAGGDEANIFAGDLFKMYTKFAEDLNFKLKVISSSAASVGGFTQIVFSIKGDKAYSKLKFESGVHRVQRVPVTESAGRIHTSTATVTVMPEIDDSVDIEIKPQDLQIDTYRSSGAGGQSVNTTDSAVRITHLPTGIVVSSQDERSQIANRETALTVLKSKLYDLEIQKKQQEEAGYRKLAGHGDRSEKIRTYNYPQDRVTDHRIGYSTSLKQVMEGKLLTIIEALLTEEQNQKIKESGL", "aa_hexdigest": "ca45339f46f8993cc611d5a44d19a4dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX0", "query_cov": 1.0, "subject_cov": 0.9946380697050938, "identity": 1.0, "score": 692.0, "evalue": 4.14e-251, "valid": true, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "IGIGPGHMMC_1512", "locus": "GCA000148625_01506" }, { "type": "cds", "sequence": "contig_1", "start": 859661, "stop": 860149, "strand": "+", "frame": 2, "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "EC:2.1.1.297", "GO:0006412", "GO:0008757", "GO:0018364", "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4", "UniRef:UniRef90_C4XFW9" ], "nt": "ATGAATAACGAAGAAACATTAATTTTAGAAAAAAAGCGGTATGGTTTGGAACCTAAAATTACTGAAAAAGAATATGAATTATTAAGGCAAAATTATCCAATTCAATTAATAATGGGCTATGTTGAATACTTAAATACTAGAATTAATTTAAGAGAACAAGTTTTAATTCCAAGATATGAAACTGAAGAAATGGTTGATATTTATCTAAAAAAATTTGCAGCTGAAAATCAAGAAGTTTTAGATCTTTGTTGTGGTTCAGGTTTTATAGGTTTAGCAATTAAGAAAAACTTACGTTCAGCTAAAGTAACTTTAGTAGATATTAGTGATGAAGCTATTAAACAAACCATTGAAAATTCTATAGTAAATTTTGGTTTTAATCATGACTTAAAAATTTACCAAAGTGACTTGTTTACTAATGTTAAGGGAAGATTTGATGTTATCATTTCAAATCCACCATATTTAAATTGCAATGACAAAAGTTTAGACTGA", "aa": "MNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKKFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLD", "aa_hexdigest": "835035e7cf32ce8b1433fe0f586f21ad", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "prmC" ], "truncated": "3-prime", "pseudogene": { "start": 859649, "stop": 860374, "observations": { "mutation": [ 860147, 860201, 860258, 860297 ], "stop": [ 860147, 860201, 860258, 860297 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW9", "query_cov": 1.0, "subject_cov": 0.6721991701244814, "identity": 0.9940000000000001, "score": 320.0, "evalue": 2.26e-109, "gene_start": 1, "gene_end": 162, "reference_start": 5, "reference_end": 166, "reference_sequence": "MAYKMNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKEFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLDWKALSFEPKIALCAPEDGWYFYDKILKKYKNYLKKNGWLIMEINPQHIEKWKQIKGAQIKKDINGKYRFVFIQNI" }, "paralog": false, "identity": 0.975103734439834, "score": 468.0, "evalue": 4.36e-170, "description": "Internal stop codon at 860147, 860201, 860258, 860297. Nonsense mutation around 860147, 860201, 860258, 860297.", "psc": { "uniref90_id": "UniRef90_C4XFW9", "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "ec_ids": [ "2.1.1.297" ], "uniref50_id": "UniRef50_A0A3P8KWM4", "go_ids": [ "GO:0006412", "GO:0008757", "GO:0018364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW9", "EC:2.1.1.297", "UniRef:UniRef50_A0A3P8KWM4", "GO:0006412", "GO:0008757", "GO:0018364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KWM4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" } }, "id": "IGIGPGHMMC_1513", "locus": "GCA000148625_01507" }, { "type": "cds", "sequence": "contig_1", "start": 860398, "stop": 860631, "strand": "+", "frame": 1, "gene": null, "product": "Carrier domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354978.1", "SO:0001217", "UniParc:UPI0001E33019", "UniRef:UniRef100_C4XFW8", "UniRef:UniRef50_A0A3P8MEJ4", "UniRef:UniRef90_C4XFW8" ], "nt": "ATGGAAAAAAATAAAATTAAAGAAATTGTTATTGCAAGATTAGACAAACTTTCAAAGAAAAAGGTAACTTTTACTTCAACACTCGAAGAATTAAAAATTGATTCATTATCACTTGCTGAATTAATTTACGAAAGTGAAATAGAATTCAAAATTAGAATTGAAGATGAAGTGCTTTTAAAAATTAAAAATGTTAATGATATTATTGATGCAATAGAAAATGCTAAGAAAATTTAA", "aa": "MEKNKIKEIVIARLDKLSKKKVTFTSTLEELKIDSLSLAELIYESEIEFKIRIEDEVLLKIKNVNDIIDAIENAKKI", "aa_hexdigest": "612627cfa794abc55c1790cbafd3c523", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33019", "ncbi_nrp_id": "WP_013354978.1", "uniref100_id": "UniRef100_C4XFW8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33019", "RefSeq:WP_013354978.1", "UniRef:UniRef100_C4XFW8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW8", "uniref90_id": "UniRef90_C4XFW8" }, "psc": { "uniref90_id": "UniRef90_C4XFW8", "product": "Carrier domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MEJ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEJ4" ], "product": "Acyl carrier protein" }, "genes": [], "id": "IGIGPGHMMC_1514", "locus": "GCA000148625_01508" }, { "type": "tRNA", "sequence": "contig_1", "start": 860664, "stop": 860738, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 73.7, "nt": "AGGAGTATAGTTCAATGGTAGAACAACGGGCTTCAACCCCGTGTGTTGTGGGTTCGAGTCCTGCTACTCCTGCCA", "db_xrefs": [], "id": "IGIGPGHMMC_14", "locus": "GCA000148625_01509" }, { "type": "cds", "sequence": "contig_1", "start": 861115, "stop": 861768, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCATATGGTGTTGAACAAGCTATGTCATCTAAAGCAGACAAACTAGAATTCCTTGAAAAATTAATGTCTGTTGGTGGTGCTAAAATAAAAGAACATGATTCAGCTATTTCCTTTATTAATGCTTATTTATTCAAGATGTTAATTTTCGAAAATGTTAAAGTTTATGAAAGACTTAATCGATTTGACAAAGTAGAATTTACATATGATCAAGTAAATAACAAAGTTGTTGTTAAATTGGTATTTGGATATGAAACACATGTATTTAATATTGATAGCAATAAATTTGACAAGAAAGTTTTAAATGTAATTAAAGTTTTTGCAAACAATGAGTTAAATAAATTTATTGATGAAATTATAAAAAATAATTGCATTAAATTAAAAGATAATAAGTATTATGATGAAAATTTACATGATAGAGATCTTGAAAAAGTATTTGAAATTGATTATACAAAAACGACAGATAAAGGCAACAGATTTAAAGTTAGAATTATTAGTCATAGTTTAAATAATCTTCAAATATCAGTAATAGATACCAAAATGAGAGAAATGTATGGATATAGTTTACCTTATGTAACCGATGGAATTAATTTAGATTTTGATATAGATAAGTTTCTAAGCCCAGTAGCTTCACAACTTAAAGGTATTTGA", "aa": "MSAYGVEQAMSSKADKLEFLEKLMSVGGAKIKEHDSAISFINAYLFKMLIFENVKVYERLNRFDKVEFTYDQVNNKVVVKLVFGYETHVFNIDSNKFDKKVLNVIKVFANNELNKFIDEIIKNNCIKLKDNKYYDENLHDRDLEKVFEIDYTKTTDKGNRFKVRIISHSLNNLQISVIDTKMREMYGYSLPYVTDGINLDFDIDKFLSPVASQLKGI", "aa_hexdigest": "fced11a4ab6d6abd989c03d6f247d26e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25304.79180000002, "isoelectric_point": 6.914542961120605 }, "id": "IGIGPGHMMC_1515", "locus": "GCA000148625_01510" }, { "type": "cds", "sequence": "contig_1", "start": 861916, "stop": 862428, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAATTAAATTAACACATAATTCAATTAATTCGCCAAAAACCTTTGATCCAAAAATTAATAATAGTTTTCCATTCACAGATTCGAAACTTGAAGAATATGTTAAAACAGACGATTTTAAACACAATTTAGAAGCAAATATTAAAGAAAACTTTTATTTACTTGATTTCATTAAAAAAGAAACTTCTGATAATAAAAAAGATATAAGCAAATTTGTACTTTCTGATTTAGAACTACAAAAAGAATATGATTCAATATTGTTAGATGAAGAATCAAAAAAGATCATTGTTACAAATATTGAAAATTATTTAAATAATAAATTTGATTCTAAATTTAATAAATTTTTAAATTCATATTTCAAACAAGCAGAAATAGCTAGAAAGAACAAAGCGATTAAAGATGATCAAAAATTGTTTAGATATACAATTAAAGAATTCTCAAATATTGATGCCTATCAATCTAAAATTAAAATTCAAGTTGAAATATTCAAAATAGAAGCTAAGGCTTGA", "aa": "MEAIKLTHNSINSPKTFDPKINNSFPFTDSKLEEYVKTDDFKHNLEANIKENFYLLDFIKKETSDNKKDISKFVLSDLELQKEYDSILLDEESKKIIVTNIENYLNNKFDSKFNKFLNSYFKQAEIARKNKAIKDDQKLFRYTIKEFSNIDAYQSKIKIQVEIFKIEAKA", "aa_hexdigest": "1914aaf09749d3d7c07f41e9e9a47405", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.6631, "isoelectric_point": 7.748711967468262 }, "id": "IGIGPGHMMC_1516", "locus": "GCA000148625_01511" }, { "type": "cds", "sequence": "contig_1", "start": 862594, "stop": 862701, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAGTAATCTTTTTTATACAGTATCAAATAAATTTAAAGCAATAGATTTTACAGATCAAAGTGCTTTAAATGAAATTCAAAGTTATTATGATAATGAATGA", "aa": "MNGSNLFYTVSNKFKAIDFTDQSALNEIQSYYDNE", "aa_hexdigest": "03a5e017bc5a35d1e2d864163aa158e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4068.344, "isoelectric_point": 4.295857048034668 }, "id": "IGIGPGHMMC_1517", "locus": "GCA000148625_01512" }, { "type": "cds", "sequence": "contig_1", "start": 862702, "stop": 862842, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTTAATAACTTTAAAGATTTAAATCCACTTATTAAATTAGTAGACAGATTGAATAAGTCAATGGTAGCTTATATTAATCCAAATGAAAGTCTTGATACATTTAATGAATCATTAGGACGTCAATTAAAATCTTGA", "aa": "MQFNNFKDLNPLIKLVDRLNKSMVAYINPNESLDTFNESLGRQLKS", "aa_hexdigest": "7abc8d783cdf9c2038af64404423c54f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5357.082399999999, "isoelectric_point": 8.139600944519042 }, "id": "IGIGPGHMMC_1518", "locus": "GCA000148625_01513" }, { "type": "cds", "sequence": "contig_1", "start": 863020, "stop": 863340, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACGAGCTTTTATATATAATAAAACTAAATTTGTTGTAACTGATTACACAATTAATGGTGATAAAGTAGAATTAAAATTTAAACTTTGTCGTAATTTAGATGATAAAAGTAAAAAGTCATTTTATGAAATATCAACAAAAGAATTCACAAAAGAAATTACTTTAGCTACAGAAAAAACAACAGAAAAAGCATATTTGGACAAACTAATTGATAATTATGACTTTTCACTTCCAGAAGGCTTACAACAATATCATGCCCATAAAGTTCTTTCTCAATTAAAACAAAAACAAATAGGATTTTTTGAACAAAAAAAATGA", "aa": "MERAFIYNKTKFVVTDYTINGDKVELKFKLCRNLDDKSKKSFYEISTKEFTKEITLATEKTTEKAYLDKLIDNYDFSLPEGLQQYHAHKVLSQLKQKQIGFFEQKK", "aa_hexdigest": "17b454cd7f35128e16eb2c479cc310fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12600.306499999988, "isoelectric_point": 9.060338401794432 }, "id": "IGIGPGHMMC_1519", "locus": "GCA000148625_01514" }, { "type": "cds", "sequence": "contig_1", "start": 863431, "stop": 863583, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCACTACACAAAAAAGACCAACATTTAGAATATCTAAAGAATTAGATGCAATTTTTGACAAAGCAAAAGAGTTTTATCTAAAACCAGAAAACTTCGATATTTACACAGGAGAATTTAAAACACAAGTTTATGGAAAATATAGTTCTTGA", "aa": "MPTTQKRPTFRISKELDAIFDKAKEFYLKPENFDIYTGEFKTQVYGKYSS", "aa_hexdigest": "0a849c2c74d04a35bfc30c682be98b1b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5958.704200000001, "isoelectric_point": 9.003799629211429 }, "id": "IGIGPGHMMC_1520", "locus": "GCA000148625_01515" }, { "type": "cds", "sequence": "contig_1", "start": 863752, "stop": 864021, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATACTGCAACAGGCGATTTATATATATCGTTTAAATTAAGAGAAAAAGCAAATAATATTTCTGTTGACGGTTATTGTAAAGTTCAAGGTTTCAAACCATTTGATAGTGTTAAAAATCCTGATTTAATAGATGTAGCTAATGCAGCAAAAGCTGTAAATGTTGCGTATGAAGATAAAGGTAGCTTTTCTATTTATGAATATAATGCGAATTTAGTTAAATTTGAAAATTATGATTCATCTATTTATGATTTAAGCCTAGAATCTTGA", "aa": "MDTATGDLYISFKLREKANNISVDGYCKVQGFKPFDSVKNPDLIDVANAAKAVNVAYEDKGSFSIYEYNANLVKFENYDSSIYDLSLES", "aa_hexdigest": "e8eeb3464227932a61b6f2ec77407e02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9949.9192, "isoelectric_point": 4.4735925674438475 }, "id": "IGIGPGHMMC_1521", "locus": "GCA000148625_01516" }, { "type": "cds", "sequence": "contig_1", "start": 864334, "stop": 864606, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAAAGAAAATTATTCTTAATCCTATCTTCATTAAGTACATTACCTTTTGCTTTTGTAGCAGGTAGTTGTGTTGGTAAAGATAATGGTGGAACAACCGGTAAACCAACTAGTGAAGAAACAAAAGCTGATTTAAATAAACTTGCAAAAAATATTGTTTTTGATGTTAAAAATAAACAAGATAAATTTGCAAAGGATATTAAAAACATATCAAATTTAGTTGTATCAAATTTAGATTTAAATAAATATGAATTAATACATTGA", "aa": "MKNKKRKLFLILSSLSTLPFAFVAGSCVGKDNGGTTGKPTSEETKADLNKLAKNIVFDVKNKQDKFAKDIKNISNLVVSNLDLNKYELIH", "aa_hexdigest": "01c14e5c21a633209d8d8915430d3261", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9955.469499999994, "isoelectric_point": 9.678977012634277 }, "id": "IGIGPGHMMC_1522", "locus": "GCA000148625_01517" }, { "type": "cds", "sequence": "contig_1", "start": 864883, "stop": 865590, "strand": "+", "frame": 1, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013354980.1", "SO:0001217", "UniParc:UPI0001E3301B", "UniRef:UniRef100_C4XFW6", "UniRef:UniRef50_A0A449B495", "UniRef:UniRef90_C4XFW6" ], "nt": "ATGTCAACACACATCAGCGCAAAAAAAGGTGAAATAGCAAGAATTTGTTTTCTTTCAGGAGACCCTTTAAGATCAAAATTTATGGCTCATAAATATTTAAAAAATGTAAAAGAAGTAAGCTCAGTTAGAAATGAAAATTACTACACAGGAGAATATAGAGGTATCAAAGTTACTTTTGGAGGACATGGAATGGGTATGTTCTCAATTGGAGCTTATGCTCATGAACTTTATAATGAATATGGAGTAGATGTTATTATTCGTTTAGGATCAACTGGTTCATATTATGACGAACACAATGTTATGGATACAGTAATTATTAATAGAGCTTTTAGCGACAACATTTCAATAGCTCAATTAATAAATAAAGAAAATACATATGAATACTTTCCAGACAAAGGTGTAACAGATGAATTAATTAAGGCAGCTAAAAAATTAAACAAAAAAGCACCAGTAGTTTCAGTACACTCAACAGATGTTTTCTATGCAACTAGACCTTTAGAGGAAACTAAGAAAGTATCAAAATGTCAAGTAGTTGAAGCAGAAAGTTATGCGCTTTTTGCAGAAGCTAAAAGAAGCAATAAAAAAGCCGCAACCATTTTACAAGTTAGTGACTCTTTAGCTAAAATGGAATTTAGTGATTATAAAACTAGAGAAACAGGATTTACAGAAATGTTCGAAATAGCTTTGGAAGCATTAAAAAATCTATAA", "aa": "MSTHISAKKGEIARICFLSGDPLRSKFMAHKYLKNVKEVSSVRNENYYTGEYRGIKVTFGGHGMGMFSIGAYAHELYNEYGVDVIIRLGSTGSYYDEHNVMDTVIINRAFSDNISIAQLINKENTYEYFPDKGVTDELIKAAKKLNKKAPVVSVHSTDVFYATRPLEETKKVSKCQVVEAESYALFAEAKRSNKKAATILQVSDSLAKMEFSDYKTRETGFTEMFEIALEALKNL", "aa_hexdigest": "3f221302d8220e1f34074f361ffa8ba9", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3301B", "ncbi_nrp_id": "WP_013354980.1", "uniref100_id": "UniRef100_C4XFW6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3301B", "RefSeq:WP_013354980.1", "UniRef:UniRef100_C4XFW6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW6", "uniref90_id": "UniRef90_C4XFW6" }, "psc": { "uniref90_id": "UniRef90_C4XFW6", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449B495", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_A0A449B495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B495" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "IGIGPGHMMC_1523", "locus": "GCA000148625_01518" }, { "type": "cds", "sequence": "contig_1", "start": 866551, "stop": 866901, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGTCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTTTTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALVLPKIFGDLSPFLKYYFNFFIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "097b322b767dca169ee8e2a053647a07", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13641.180399999976, "isoelectric_point": 9.912288093566893 }, "id": "IGIGPGHMMC_1524", "locus": "GCA000148625_01519" }, { "type": "cds", "sequence": "contig_1", "start": 867281, "stop": 867712, "strand": "-", "frame": 1, "gene": null, "product": "ABC-2 type transporter transmembrane domain-containing protein", "db_xrefs": [ "KEGG:K11051", "SO:0001217", "UniRef:UniRef50_C4XFW5", "UniRef:UniRef90_C4XFW5" ], "nt": "ATGATTTCAACTACAACGACAATTGGTTGTATTACAGTTAGTTTAGGTGCTTCAAGTTTCATGGTTGAAGATATAGAATCAAAAGCCTATAAAGACATTTATGCTTCGCCAGTTAATAAATCTTCAATAATTATTGGAAACGCTATTTCTATTTATGCTGTTACTGTAATTATGTCAACTGTATCATTTATTATTAGTTTTGTGTATGCATTATGCTTTGGAGCAAAATTCTTATCATTTTTACAAATTGTTGAAGTAATTGGATTGATTTTAGTTTCAAGTTTAATAGGAAGTAGTTTTAGCTTTTTAATTGTTTACTTCATTAAAAAACATAATGTTTTTGCAGCTATCAATGCTTTAATTGGATCTATAGCAGGTTTTATAATTGGTGGTTATTCAACAATGGAATCACTGCCTGATGCGCTTCAATGA", "aa": "MISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQ", "aa_hexdigest": "dd84a2f864aefc33be0e99c0a1821b88", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 867032, "stop": 867925, "observations": { "mutation": [ 867124, 867283, 867715, 867763 ], "stop": [ 867124, 867283, 867715, 867763 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW5", "query_cov": 1.0, "subject_cov": 0.48148148148148145, "identity": 1.0, "score": 256.0, "evalue": 5.89e-84, "gene_start": 1, "gene_end": 143, "reference_start": 72, "reference_end": 214, "reference_sequence": "MERRLDNMGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKGWVIEDKSKSAREIMNLWMISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQWIIKCFPASHANVLFRQTLVSPAIDRLISDPTHIEQFKKIMGCFYKFNDYQMQWYVNFIIVAGTSLLLYSIAFGVQFTKKIKN" }, "paralog": false, "identity": 0.9831649831649831, "score": 553.0, "evalue": 1.78e-203, "description": "Internal stop codon at 867124, 867283, 867715, 867763. Nonsense mutation around 867124, 867283, 867715, 867763.", "psc": { "uniref90_id": "UniRef90_C4XFW5", "product": "ABC-2 type transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_C4XFW5", "kegg_orthology_id": "K11051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW5", "UniRef:UniRef50_C4XFW5", "KEGG:K11051" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW5" ], "product": "ABC-2 type transporter transmembrane domain-containing protein" } }, "id": "IGIGPGHMMC_1525", "locus": "GCA000148625_01520" }, { "type": "cds", "sequence": "contig_1", "start": 867761, "stop": 867904, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCAATAATTAAAAGAAATATTAAAAAATATTTCATGGACAAAACTAATGTTTTCTTTTCTTTATTAAGTGTTATTTTAGTCTTCTTTATAGTTGCAGTTTTTTTAGGTAAATCAATTAGTGATGGACTTAAAGGATGA", "aa": "MGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKG", "aa_hexdigest": "047a109a396dc4c8a91c232e14e0c49c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5270.385499999999, "isoelectric_point": 10.121810340881346 }, "id": "IGIGPGHMMC_1526", "locus": "GCA000148625_01521" }, { "type": "cds", "sequence": "contig_1", "start": 867906, "stop": 868241, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGAAAAAGCTGACTATGTTGTGATTATGGATAATGGAAAAATTGTGGCTAAGAATACTCCTGCAAAACTAAAAGAAGAATATACAAAAGATAGGTTAAAAGTATATCTTGAAAATGATGTAAAGTACTTTAAAACAGTTGAATTGTTAAAAAACAAAGGTTTAAAATTCAAAGAATTTAAAGATTATATTGATGTATATTTAGACTCTTCGTTTAAAGCTATAGATTTGGCAAATCAACTTAAAGATTTGATATCTTCTTTCGAAGTTTTAAAGGGTGATATGGATGATGCATTTATCAATATAACTGGAAAGGAGATTAGATAA", "aa": "MEEAEKADYVVIMDNGKIVAKNTPAKLKEEYTKDRLKVYLENDVKYFKTVELLKNKGLKFKEFKDYIDVYLDSSFKAIDLANQLKDLISSFEVLKGDMDDAFINITGKEIR", "aa_hexdigest": "3f8909d0ac50af35b5d081c8f0168cc8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12896.724599999987, "isoelectric_point": 5.269167900085448 }, "id": "IGIGPGHMMC_1527", "locus": "GCA000148625_01522" }, { "type": "cds", "sequence": "contig_1", "start": 868302, "stop": 868826, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "nt": "ATGTCTGAAATAATAAAAGTTGAAAATTTAAAAAAATCATATGGCAAAGTTGAAGCTGTCAAAGGTATTTCTTTCATTGTAAAAGAAGGAGAGCTTTTTGCATTTTTAGGGCCTAATGGTGCAGGCAAAAGTACCACAATTGATACCTTGTGTACATTGAATGATTATAATGAAGGCCAAATAACAATTTGTGGTTTTGATTTAAAGAAACAAAGTGATGAAATCAAAAAACAAATTGGTGTGGTTTTTCAAGATAGTGTATTAGATCGAAAATTAAGTGTTAGAGATAATTTAACAATTAGAGCAGCATATTATTATCGTAAAAAAGCAGATATAAAAGCAGCTTTTGAAAGGGTTGTAAAAGATACTCAAATTGAAGATATTTTGAAAAGAAAATATGAAAAACTTTCAGGTGGTCAAAGACGTCGTGTGGATATAGCTAGAGCCTTATTAAATTATCCAAAAATTTTATTTTTAGATGAACCAACAACAGGATTAGATCCTCAAACTAGAAAGCTTATTTGA", "aa": "MSEIIKVENLKKSYGKVEAVKGISFIVKEGELFAFLGPNGAGKSTTIDTLCTLNDYNEGQITICGFDLKKQSDEIKKQIGVVFQDSVLDRKLSVRDNLTIRAAYYYRKKADIKAAFERVVKDTQIEDILKRKYEKLSGGQRRRVDIARALLNYPKILFLDEPTTGLDPQTRKLI", "aa_hexdigest": "bb0ae2805b7984ffea7308ee2bf28558", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002079168B", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.632, "score": 223.0, "evalue": 2.44e-71, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI002079168B" }, "pscc": { "uniref50_id": "UniRef50_UPI002079168B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "IGIGPGHMMC_1528", "locus": "GCA000148625_01523" }, { "type": "cds", "sequence": "contig_1", "start": 869006, "stop": 870820, "strand": "+", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "RefSeq:WP_013354984.1", "SO:0001217", "UniParc:UPI0001E3301F", "UniRef:UniRef100_UPI0001E3301F", "UniRef:UniRef50_A0A6M4J8T6", "UniRef:UniRef90_C4XFW3" ], "nt": "ATGGCATATAAAGCACTTTATCGAAAATATCGTCCGAAGTCTTTTGATGATGTTAAGGGTCAAGATCATATTGTGCAAACATTAAAAAATATCATTTTAAATCACAAGATAAGTCATGCATACTTATTTAGTGGACCAAGAGGTGTTGGTAAAACTTCAGTTGCTAAAATTTTTGCTAATGTAGTTAACTGTGGTCATGGTGAAGATTTAACTAAACTTTGTGAAAGTTGTATTGAAAAAGCGGATCAAAATTTTGATGTTATCGAAATGGATGCTGCTTCAAATAATGGTGTTAATGAAATTAGAGATTTAAGAGATAAAATCCAACATTCACCAGCAAGTGGAAACTACAAAGTTTATATAATCGATGAAGTTCACATGCTTTCAAAAGGAGCTTTTAATGCACTTTTAAAAACATTAGAAGAACCACCAGCACATGCAATATTTATTTTAGCAACAACTGATCCTCAAAAAATACCTCTAACAATTCTTTCAAGAGTCCAAAGATATAATTTTAGGAAAATATCAAACCAAGTTCTAATTAACCAAATTAAAGAGGTCTTAGATAAAGAAAAAATTAAATATGAAGATTCAACCATAGCTTATATTGCAAGATTAGCAACTGGTGGTATGCGTGATGCACTTTCAATAGTCGACCAAGCTGCAGCTTATGGTAACGGCAAAATATCGCTCAAAGATGTTATGTATGCTTTTGGAATTACTTCAAATGAAAGCTTAATTCAAATTGTAAACTACCTTTATCAAGGCAAAATCAAGGAAGTCTTAATTTTATTTAATAACCTTAAAAATGCGGGAATTGATTCAAATCAATTTGTTCAAAGCTTAATAGAATTGTTCAAGGATTACTTGATTTATGCAAAATCAAATGATGCTAATTTATTAGAACTTTTATCTCTTGAAGAATTAAATACTTTGAAAATTGATGTTAATTTTGCATTCAATTCATCTGAAAAACTTTATAAATTATTCAAAGAATTAATTTTTTCAGAAAACCCATTCCAATTAATCGAACTTTATTTAATTAAACTTAATCAAACTAATGAATTACAAAATAGTAAGGAGCAAACTATGTCAATTGATGTAAACAAAAAGAAACCTAAAAAAGATGACACAATCAAAACAGTTTTAAATCAAACTCAAGAATTAGTACTTGAAGCTAATCGTGACGCTGATGACAATTTTAGCCTAGATCAAGATCTTCTCAGTAGCAATTTTGAAATAGAAGAAGATTATGATGATGGTTTAGTAAGTACAGCAGAAATTCAACTAGATGATGAAATTCAAAATATGCCAAAACCAGTAGTAATTCCTGCTTTTGACTCAAAATACAAAAACTTGAAAACATTCAAATCAAACTTAAGTAAAAAAGATTTAATCAACTTACTTAAACAATCAACATTAACATTTTTAAATAGATACAAATCAAATTTAGATATTGTGATTAACACAGTTCAAAATCCAATTTACAGTGATTTAATTACAGTACTTAAAAAATGTAAAATTGCCGCAGCAGGCCCTAATTACATGATTTTTATCTCAAAAAATGTACCAGAATTAGTTTATTTACAAGAAAATGGTTACAACTCAAATATTCAAAAATTTATTCAAGATTACTTTGAAGATTACAAACACTTATACTGCATTATGAGTGATGATTACAAAGAAACAGCAAAAGATTTTGCAGCTAAAATGAAAGATGCAAAATTTGACACAAGCTTTACAGTACCAGGACCAGTTGTTTTACAAAATGTAAAGAGTCCAACAGGCAAATTATTTGATAAATTCAAAAAATAA", "aa": "MAYKALYRKYRPKSFDDVKGQDHIVQTLKNIILNHKISHAYLFSGPRGVGKTSVAKIFANVVNCGHGEDLTKLCESCIEKADQNFDVIEMDAASNNGVNEIRDLRDKIQHSPASGNYKVYIIDEVHMLSKGAFNALLKTLEEPPAHAIFILATTDPQKIPLTILSRVQRYNFRKISNQVLINQIKEVLDKEKIKYEDSTIAYIARLATGGMRDALSIVDQAAAYGNGKISLKDVMYAFGITSNESLIQIVNYLYQGKIKEVLILFNNLKNAGIDSNQFVQSLIELFKDYLIYAKSNDANLLELLSLEELNTLKIDVNFAFNSSEKLYKLFKELIFSENPFQLIELYLIKLNQTNELQNSKEQTMSIDVNKKKPKKDDTIKTVLNQTQELVLEANRDADDNFSLDQDLLSSNFEIEEDYDDGLVSTAEIQLDDEIQNMPKPVVIPAFDSKYKNLKTFKSNLSKKDLINLLKQSTLTFLNRYKSNLDIVINTVQNPIYSDLITVLKKCKIAAAGPNYMIFISKNVPELVYLQENGYNSNIQKFIQDYFEDYKHLYCIMSDDYKETAKDFAAKMKDAKFDTSFTVPGPVVLQNVKSPTGKLFDKFKK", "aa_hexdigest": "ce6e4e90222ff5253a2dd31bab2b71d8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E3301F", "ncbi_nrp_id": "WP_013354984.1", "uniref100_id": "UniRef100_UPI0001E3301F", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3301F", "RefSeq:WP_013354984.1", "UniRef:UniRef100_UPI0001E3301F" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E3301F", "uniref90_id": "UniRef90_C4XFW3" }, "psc": { "uniref90_id": "UniRef90_C4XFW3", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A6M4J8T6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4J8T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4J8T6" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "IGIGPGHMMC_1529", "locus": "GCA000148625_01524" }, { "type": "cds", "sequence": "contig_1", "start": 870868, "stop": 871164, "strand": "+", "frame": 1, "gene": null, "product": "Nucleoid-associated protein MBIO_0769", "db_xrefs": [ "KEGG:K09747", "RefSeq:WP_013354985.1", "SO:0001217", "UniParc:UPI0001E33020", "UniRef:UniRef100_C4XFW2", "UniRef:UniRef50_C4XFW2", "UniRef:UniRef90_C4XFW2" ], "nt": "ATGGATCAAGCAATGTTAAGAAAGATGCAAAAATTGCAACAAGAAGTTCAAGAAAAAACTGAACAATTCATGGAAGAAACATTTAAATTAGAAAAACATGGAATTGAAGTTACAGCTAAAGGAAGCAAAAAAATAGTTTCAGTAAAAATCAAAGATTCTATTCTTTTAGACCCAGATGATGCTGAAACTTTAGAAGACTTGTTATCACTTGTGATTAATGAGTTATTTGACACAATTGATGAAGAACAAGAAAAATTAATGCCAGCTATGCCTGGTTTAGGTGGACTTGGCTTCTAA", "aa": "MDQAMLRKMQKLQQEVQEKTEQFMEETFKLEKHGIEVTAKGSKKIVSVKIKDSILLDPDDAETLEDLLSLVINELFDTIDEEQEKLMPAMPGLGGLGF", "aa_hexdigest": "ab897048bca2ffa3e26156e29058872f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33020", "ncbi_nrp_id": "WP_013354985.1", "uniref100_id": "UniRef100_C4XFW2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33020", "RefSeq:WP_013354985.1", "UniRef:UniRef100_C4XFW2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW2", "uniref90_id": "UniRef90_C4XFW2" }, "psc": { "uniref90_id": "UniRef90_C4XFW2", "product": "Nucleoid-associated protein MBIO_0769", "uniref50_id": "UniRef50_C4XFW2", "kegg_orthology_id": "K09747" }, "pscc": { "uniref50_id": "UniRef50_C4XFW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW2" ], "product": "Nucleoid-associated protein MBIO_0769" }, "genes": [], "id": "IGIGPGHMMC_1530", "locus": "GCA000148625_01525" }, { "type": "cds", "sequence": "contig_1", "start": 871167, "stop": 871757, "strand": "+", "frame": 3, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "KEGG:K06187", "RefSeq:WP_013354986.1", "SO:0001217", "UniParc:UPI0001A8C12E", "UniRef:UniRef100_C4XFW1", "UniRef:UniRef50_N9VD56", "UniRef:UniRef90_C4XFW1" ], "nt": "ATGAAAATTGAAACAATTGAAAAACTTAATCAAGTTTTAACAACAATTCCAGGAATTAGTAAAAAGCAAGCAGACAAAATGTCAAACTTTTTCTTAAAACAAGATCAAGAATATATTGACAATTTAGTTAAGTCAATTAATGATCTTAAAACCAACATTTCATATTGTTCTATTTGTAACTTTTTAAAAGAAGGTGGCAAATGTTTAGTATGTGATGATAAGAGCAGATACAACTTTTTAATGATAGTTGAAAATGCTGCAACTTTAAGAAAAATTGATGACATGAATTTTTACAATGGCTACTACTATGTTTTGCCTTATTTGTTTAATTTAAAGAATAATAAGGAAATTAAAAACTATGATTACAAAGAATTAATAAACTATATTCAACATAAAAAATTTGATGAAGTAATCATAGTTTTGAGCCCTTCTTTAGAAGGCGAAATGACAACAGGTCATTTATTAAAATTGATTAATAGCCAATTTAAAATTAAAGTAACAAGAGCAGCTATTGGCATGCCTATGGGTTCAAATTTAGAATATCTTGATACTTTTACAATCAAACAATCTATTGAAAATAGAAACAAATAA", "aa": "MKIETIEKLNQVLTTIPGISKKQADKMSNFFLKQDQEYIDNLVKSINDLKTNISYCSICNFLKEGGKCLVCDDKSRYNFLMIVENAATLRKIDDMNFYNGYYYVLPYLFNLKNNKEIKNYDYKELINYIQHKKFDEVIIVLSPSLEGEMTTGHLLKLINSQFKIKVTRAAIGMPMGSNLEYLDTFTIKQSIENRNK", "aa_hexdigest": "723c7d4573e29f587df86bd8be7458b4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12E", "ncbi_nrp_id": "WP_013354986.1", "uniref100_id": "UniRef100_C4XFW1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12E", "RefSeq:WP_013354986.1", "UniRef:UniRef100_C4XFW1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW1", "uniref90_id": "UniRef90_C4XFW1" }, "psc": { "uniref90_id": "UniRef90_C4XFW1", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_N9VD56", "kegg_orthology_id": "K06187" }, "pscc": { "uniref50_id": "UniRef50_N9VD56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VD56" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "IGIGPGHMMC_1531", "locus": "GCA000148625_01526" }, { "type": "cds", "sequence": "contig_1", "start": 871777, "stop": 872031, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02223.23" ], "nt": "ATGTTTATTACTTTTGAAGGACCAGATGGTAGTGGTAAAACTACAATAATTAACCGCCTTATTATTGAATTAATTGAAAAATATCCAAAGCTTGCATTAAATTATGTTTTGACTCGTGAACCGGGTGGCAAAGACATAATTGAAGCAGAAAAAATTAGATCATTAATTTTGGATAAAGATTCAAAGCTTTCTTCAGTTTCTGAAGCTCTTTTATATACAACTAGTCGTCGAATTCATCTTGAAAGAGTAGTATGA", "aa": "MFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVV", "aa_hexdigest": "00831f06906d099994faeb4c3b971296", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02223.23", "name": "Thymidylate_kin", "length": 79, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9113924050632911, "evalue": 2.0571468289143808e-14, "score": 49.67196273803711, "start": 5, "stop": 83 } ], "seq_stats": { "molecular_weight": 9517.996399999996, "isoelectric_point": 8.0078275680542 }, "id": "IGIGPGHMMC_1532", "locus": "GCA000148625_01527" }, { "type": "cds", "sequence": "contig_1", "start": 872059, "stop": 872415, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_Q4A6Z8", "UniRef:UniRef90_C4XFW0" ], "nt": "TTGTGCGATCGCTATGTCGACAGCTTTTTTGCATATCAAGGTTATGCTCGTAATTTAGGTATTACTTTTGTTAAAAAACTTACTAATTTAATAATTGATAAAACTATGCCTGATATTACAATCTTTTTTGAAATCAAACCTGAAGAAAGTAAAATCAGAAGAGAACAAATAAGAATTGTTAGTGACAGATTAGACAGTGAAACCGAATTATTCCACCAAAAAGTTTACAAAGGTTATCATGAGCTAATTAAAAAAGAAAAAGATAGATTCATTGTTGTTGATGCTACAAAAAGTATTGAAGAAGTTTTAAAAGAATCATTTGAAAAATTAGATAAACATCCAAGATTTAAAAAATGA", "aa": "MCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKK", "aa_hexdigest": "59c1993f650aa9d68c50d1277b0c8e06", "start_type": "TTG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "both", "pseudogene": { "start": 871771, "stop": 872433, "observations": { "mutation": [ 872029, 872413 ], "stop": [ 872029, 872413 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW0", "query_cov": 1.0, "subject_cov": 0.5363636363636364, "identity": 0.992, "score": 228.0, "evalue": 3.12e-74, "gene_start": 1, "gene_end": 118, "reference_start": 97, "reference_end": 214, "reference_sequence": "MIMFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVVWPALKENRLVLCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKKWIKEFK" }, "paralog": false, "identity": 0.9863636363636363, "score": 422.0, "evalue": 4.94e-153, "description": "Internal stop codon at 872029, 872413. Nonsense mutation around 872029, 872413.", "psc": { "uniref90_id": "UniRef90_C4XFW0", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_Q4A6Z8", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW0", "EC:2.7.4.9", "UniRef:UniRef50_Q4A6Z8", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6Z8" ], "product": "Thymidylate kinase" } }, "id": "IGIGPGHMMC_1533", "locus": "GCA000148625_01528" }, { "type": "cds", "sequence": "contig_1", "start": 872433, "stop": 872951, "strand": "+", "frame": 3, "gene": null, "product": "DNA polymerase III subunit delta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9", "UniRef:UniRef90_C4XFV9" ], "nt": "ATGGTTTCAAAAAATGTCATAACTATTTTAGAAAACTCAATAAAAAATAACAAACTTAGTCACTGTTATTTACTAAAAGCTCCTTCAGGTTTAAACATCGATGATTCAATTTTATACATGATTAATAAACTTTGCAACTCACAATTGCAATCTTTAGATCTTGAAACAATGCTGCCTAATGTTCAAATTTTTGAAGATAATGGCACTGAAAATAATCTTAAAAAAGATAAAATTGTGGCCGGCTTTGAAGAATCAAGTTTATCTTCATTTGTTGAAGGGCAAAAGAAGATAACTGTATTTAAAAATGTTGAAAATGCTTCTAAAGCTGCTCTTAATTCATTACTTAAAATAATTGAAGAACCAAGTGATAATGTAGTCTTTATTTTAACAACTAATAACATAACAAAAGTTTTAGAAACCATCAAATCAAGATCAATAACAATTAACATTAATGCGCCTTCAAACGAAGAGTTAGCAGAAGAATTAAAAGGTGAAAATTTAAAAGAAGAAGCTATATGA", "aa": "MVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAI", "aa_hexdigest": "b8b186c364a1adcf83121e6898721d43", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 872430, "stop": 873254, "observations": { "mutation": [ 872949, 873159, 873165 ], "stop": [ 872949, 873159, 873165 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFV9", "query_cov": 1.0, "subject_cov": 0.5620915032679739, "identity": 1.0, "score": 318.0, "evalue": 1.26e-107, "gene_start": 1, "gene_end": 172, "reference_start": 2, "reference_end": 173, "reference_sequence": "MMVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAIWFYSHIFADLTQVKKYTNNESFKLIKDLLEAIEKSIKNPHYLYIFLSRFTKKDSKDTFVFLAMALRFVFSWVWTADAFCRKNYAKLLVKLKNIKFDLFNCFIVIDDFLKIQNTNANFFLQAEKMLIKLVESYA" }, "paralog": false, "identity": 0.9854014598540146, "score": 520.0, "evalue": 9.61e-190, "description": "Internal stop codon at 872949, 873159, 873165. Nonsense mutation around 872949, 873159, 873165.", "psc": { "uniref90_id": "UniRef90_C4XFV9", "product": "DNA polymerase III subunit delta", "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFV9", "UniRef:UniRef50_C4XFV9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9" ], "product": "DNA polymerase III subunit delta" } }, "id": "IGIGPGHMMC_1534", "locus": "GCA000148625_01529" }, { "type": "cds", "sequence": "contig_1", "start": 873343, "stop": 873867, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal RNA small subunit methyltransferase I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "nt": "ATGCCTAAAATATATATTGTTGGAACACCTATTGGAAACTTAAAAGATATAACTTTAAGAGCTCTTGAAACTCTTAAGGCTGTTGATGTTATTGCTTGCGAAGATACTCGTGTTAGTGCTAAATTATTGAACCATTATGACATTAAAAAACGTTTAATTTCATACAACAAAATCAATGAAAAAGCAAGTGCCCAAGGCCTTATTGATTTAATTGAAAAAGATGACTTAAAAATGGCTTTAATTAGTGATGCAGGTATGCCTTTAGTTAGTGATCCGGGTTTTGAATTAATTAAACAAGCAAGAGAAAAAAATATTGAATTAGAACTAATTCCAGGAGTCAATGCAGCTATTAGTGCTTTTGCTTTTAGTGGACTAAGTAATACCTTTATTTTTATGACTTTTCCTAAAGAAAAAAGTGGCCAAAGATTAGAACAAGTCAAAAGCTTCACAAGTGAACATGCATATGTTTTTTATGTAGCTCCGCATAAATTAGAAAGTTTCTTAAAAGACATTGACTCAGTGTGA", "aa": "MPKIYIVGTPIGNLKDITLRALETLKAVDVIACEDTRVSAKLLNHYDIKKRLISYNKINEKASAQGLIDLIEKDDLKMALISDAGMPLVSDPGFELIKQAREKNIELELIPGVNAAISAFAFSGLSNTFIFMTFPKEKSGQRLEQVKSFTSEHAYVFYVAPHKLESFLKDIDSV", "aa_hexdigest": "620f13f2f9abb04a43e6141dc9760c69", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0010050DA8", "query_cov": 1.0344827586206897, "subject_cov": 0.8181818181818182, "identity": 0.5, "score": 168.0, "evalue": 9.39e-50, "valid": false, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [], "id": "IGIGPGHMMC_1535", "locus": "GCA000148625_01530" }, { "type": "cds", "sequence": "contig_1", "start": 873889, "stop": 874053, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAGAGCTTACTAAAATACATGAAGAATTTATTTATGCAACCGCAACTGAACTATTAGCAAAAAACAAAACTAGTCCTTTTAAAGGTGAGATAACTTTAGTTATCAAACTAAAAGAAATTAAAAAAGAGAAAATAAATAAATATGCTCACTTAAAATAA", "aa": "MARELTKIHEEFIYATATELLAKNKTSPFKGEITLVIKLKEIKKEKINKYAHLK", "aa_hexdigest": "11e25ee0c2d353e13d5e9c128a343d0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6301.4431, "isoelectric_point": 9.647580909729005 }, "id": "IGIGPGHMMC_1536", "locus": "GCA000148625_01531" }, { "type": "cds", "sequence": "contig_1", "start": 874132, "stop": 874371, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ], "nt": "ATGAATCATATTAATATTTTTGCTCTTGGTGGGCTTGATGAAAATGGTAAAAACTGTTATGTTTTCGAATTCAATGATGACATTTACATTATTAACTCAGGAACTAAAATTCCAATCAACTCAAATAATGGAGTTGATACTTTAATTCCTTCTTTTGAATACTTAGAAAAACACAAAGATAGAATTAAAGGTATCTTTATTTCTGATGTTAAAAATGAATCGTTTAGTGCATTGCCTTGA", "aa": "MNHINIFALGGLDENGKNCYVFEFNDDIYIINSGTKIPINSNNGVDTLIPSFEYLEKHKDRIKGIFISDVKNESFSALP", "aa_hexdigest": "cf88b56f74275f1fbf1d11bd63f36936", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002201F3D4", "query_cov": 0.9746835443037974, "subject_cov": 0.875, "identity": 0.636, "score": 108.0, "evalue": 1.61e-29, "valid": false, "uniref50_id": "UniRef50_UPI002201F3D4" }, "pscc": { "uniref50_id": "UniRef50_UPI002201F3D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8909.930499999995, "isoelectric_point": 4.8840837478637695 }, "id": "IGIGPGHMMC_1537", "locus": "GCA000148625_01532" }, { "type": "cds", "sequence": "contig_1", "start": 874378, "stop": 875784, "strand": "+", "frame": 1, "gene": null, "product": "Putative hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84", "UniRef:UniRef90_C4XFV7" ], "nt": "ATGAAAATTCCTAACTTAACAATTTATACTTCAGCATTCAACAAAATCATGATCATGGATCGTTTATCAAAATATAAGATTCCAACAAAAAACTACAAAGTTATGGTTATTAATAAAGTTACACCATTTAGTGACAAACTTTTTGTTAAGCCAATCGATTTAGCTGGTTCAATGCCAGGACACATTGGTTTTGACTTCATCACACCAGATGGAGATATCTTATTTATGTTTAACTTTGTCGAAGGTGACTTAGGAATTTATGGTAAATTAAGCTTTAATGAACTTAAACAAAGATTTACAAAACGTAAAATTTTAGCTCTAGTTGTTGACTCAGGTAAAGCAAACTTTGGTGGTAAGGCTATTGAAAAAATTGGTTTACCAGAAGGTATTAGAGATGTTTTTTTAGAAACCAAAAAGAATGAAAGAATAATAGTTGGTGCTTATGACGAAGAAATGGTAGCTATCCACAAAATTTTAGAATTAGCTCAAGAAACAAGTCGTCCAGTTGTAACTTATGGTAAAACTTATGGCCAAATATTTTATTTAATTAAAAAAGCTCATCCTGAACTTAAGTTACCAGAATTAATTGATTATCGTAATGCTAACAAAGTTAAAAATGCTGTTATTTTAGTAACTGGTGCTACTGAAAGACTTTACTCAAGATTTATTAGAATAACTGACAATAATGATGTTTTTTTAAAACTTCAAAAAAGCGATACTGTTGTAATGATTGCTCCTCCTATTAATGGACTTGAAAGTCTTGAAGCTTTAACTTTAGATGAAATTGCAAGAATTACTCCAAAAATTAGTGATGTTAATGCTAATGAATTTTATAGATGTCGTCCTGCTAAACAAGACTTAATTGATTTAGTTAATGCTCTAAATCCTGAATATGTGATTCCTGTGCAAGGACTTTATCGTTACTTAAATGAAGCTACTATAGCTATAAGTGAAAATACACAAGTGAAACAAAATCATTGTTTAGTTATGCAAAATGGAAAAATTGTTCACTTTATTGATGGCAAAATGGCAACAACCAAGGGCAAAGTTAAAGAAATTGGCGACACTATTATTGATGGTTTTGGTGTTGGCGATATTAGTACTGAGGTTATTAGTGAGCGTGAAGCTTTAGGTCGTGAAGGTGTGATTTTAGTGTCTTCTCAATATAATCCAAAAACAAAATTATTAACTGGTAAATTACAAATAAATTTTGTTGGTGTCATAAACAAAGAGGAAAAAAATGAAGCTAGCGACATAATCAAAAGCACAATTGTAAAATTAATTGAAACAGAAAGTTTTAATGGAATAAAAGATTTCCAAAATGAAGCTCGTCATGCTATTAGAAAAAGAATTTACAAAACTTTTGAAAAAGAACCAATAGTAATTGTAACATTGGCTCAAATTTAA", "aa": "MKIPNLTIYTSAFNKIMIMDRLSKYKIPTKNYKVMVINKVTPFSDKLFVKPIDLAGSMPGHIGFDFITPDGDILFMFNFVEGDLGIYGKLSFNELKQRFTKRKILALVVDSGKANFGGKAIEKIGLPEGIRDVFLETKKNERIIVGAYDEEMVAIHKILELAQETSRPVVTYGKTYGQIFYLIKKAHPELKLPELIDYRNANKVKNAVILVTGATERLYSRFIRITDNNDVFLKLQKSDTVVMIAPPINGLESLEALTLDEIARITPKISDVNANEFYRCRPAKQDLIDLVNALNPEYVIPVQGLYRYLNEATIAISENTQVKQNHCLVMQNGKIVHFIDGKMATTKGKVKEIGDTIIDGFGVGDISTEVISEREALGREGVILVSSQYNPKTKLLTGKLQINFVGVINKEEKNEASDIIKSTIVKLIETESFNGIKDFQNEARHAIRKRIYKTFEKEPIVIVTLAQI", "aa_hexdigest": "cd72e8b42aa93c037ad02155857f6bc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFV7", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 0.998, "score": 914.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_A0A448ZZ84" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84" ], "product": "Putative hydrolase" }, "genes": [], "id": "IGIGPGHMMC_1538", "locus": "GCA000148625_01533" }, { "type": "cds", "sequence": "contig_1", "start": 876315, "stop": 876677, "strand": "+", "frame": 3, "gene": null, "product": "Monocytic differentiation/activation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932", "UniRef:UniRef90_P94932" ], "nt": "ATGAAAAAGTCAAAAAAAATTTTATTAGGATTGAGTCCTATTGCTGCTATTCTTCCTGCAGTAGCTGTTTCTTGTAGAAACAACGATGAATCCAATATTTCATTCAAAGAGAAAGATATTAGTAAATATACCACAACAAATGCTAATGGAAAACAAGTTGTTAAAAACGCTGAATTGTTAAAATTGAAACCAGTTCTTATTACAGATGAAGGTAAAATTGATGATAAATCATTTAACCAATCAGCTTTTGAAGCTTTAAAAGCTATAAATAAACAAACTGGTATTGAAATTAACAGTGTTGAACCTAGCTCAAACTTTGAAAGTGCTTACAACAGTGCACTTTCAGCCGGACACAAAATTTGA", "aa": "MKKSKKILLGLSPIAAILPAVAVSCRNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPSSNFESAYNSALSAGHKI", "aa_hexdigest": "2199f8bf571cb1a0d4f502d3310fa4d9", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 876315, "stop": 876873, "observations": { "insertion": [ 876657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P94932", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 0.9329999999999999, "score": 210.0, "evalue": 1.54e-67, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MKKSKKILLGLSPIAAVLPAVAVSCGNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPISNFESAYNSALQPDTKFEYLMASNTNNLLNNTLMLTEKNLKEIKSKSLVSTLILKQSTSDSTHYNSILKNLHLQQAMQLQVD" }, "paralog": false, "identity": 0.9837837837837838, "score": 344.0, "evalue": 8.39e-123, "description": "Frameshift due to insertion around 876657.", "psc": { "uniref90_id": "UniRef90_P94932", "product": "Monocytic differentiation/activation factor", "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P94932", "UniRef:UniRef50_P94932" ] }, "pscc": { "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932" ], "product": "Monocytic differentiation/activation factor" } }, "id": "IGIGPGHMMC_1539", "locus": "GCA000148625_01534" }, { "type": "cds", "sequence": "contig_1", "start": 877056, "stop": 877358, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484", "UniRef:UniRef90_UPI00217FF484" ], "nt": "ATGAACACTGTTATTAATAATGTTTTATCTTCAACACCAGCTGATGTTAAATACAACCCACATGTTATCTTATCTGTTGCTGGACCTGCTACATTTGAAACTGTAAAATTAGCAAACAAAGGTCAATATGTAATTGGTGTTGACTCAGACCAAGGCATGATTCAAGACAAAGACAGAATTCTTACATCAGTTCTAAAACACATTAAACAAGCTGTTTATGAAACATTATTAGATCTTATTCTTGAAAAAGAAGAAGGATATAAACCATATGTAGTTAAAGACAAAAAAGCAGACAAAAAATGA", "aa": "MNTVINNVLSSTPADVKYNPHVILSVAGPATFETVKLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKK", "aa_hexdigest": "bd45aae5369f5da1f1ce199e233e6d88", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 876858, "stop": 877601, "observations": { "mutation": [ 876867, 877356, 877386 ], "stop": [ 876867, 877356, 877386 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217FF484", "query_cov": 1.01, "subject_cov": 0.40725806451612906, "identity": 0.98, "score": 189.0, "evalue": 7.87e-59, "gene_start": 1, "gene_end": 100, "reference_start": 67, "reference_end": 167, "reference_sequence": "MASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLAANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKKWSHFGTQKEKWIGVAENHFSNTEEQAKINNKIKEAIKMFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK" }, "paralog": false, "identity": 0.9758064516129032, "score": 468.0, "evalue": 2.52e-171, "description": "Internal stop codon at 876867, 877356, 877386. Nonsense mutation around 876867, 877356, 877386.", "psc": { "uniref90_id": "UniRef90_UPI00217FF484", "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217FF484", "UniRef:UniRef50_UPI00217FF484" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11131.738499999994, "isoelectric_point": 7.894427680969238 }, "id": "IGIGPGHMMC_1540", "locus": "GCA000148625_01535" }, { "type": "cds", "sequence": "contig_1", "start": 877467, "stop": 877601, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAGAATTACCAGAAGATTTCGTTAAATATATTAATAGTGACAAAGCTTTAAAAGATGGTAATAAAATTGACAATGTTAGTGAAAGATTAGAAGCAATTATTTCTGCTATTAACAAGGCAGCAAAATAA", "aa": "MFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK", "aa_hexdigest": "e7960324786cd46f128a8fa539f7add7", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4967.6506, "isoelectric_point": 6.125163078308105 }, "id": "IGIGPGHMMC_1541", "locus": "GCA000148625_01536" }, { "type": "cds", "sequence": "contig_1", "start": 877747, "stop": 878097, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "6a907dccef0cbbba0bbf260e0f56003e", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13576.067399999976, "isoelectric_point": 9.912288093566893 }, "id": "IGIGPGHMMC_1542", "locus": "GCA000148625_01537" }, { "type": "cds", "sequence": "contig_1", "start": 880051, "stop": 881154, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "nt": "ATGAACGCAATTGAATTTATTAATATTTCGAAAAGTTTCGGTGTAATCAAAGCTAACCAAGATATTAATATTCAAATTAAAAAAGGTACAATTCATGCACTTATTGGTGAAAATGGTGCAGGTAAAAGTACCTTGCTATCAATTCTTTTTGGACTATATGAACCCGACCATGGAATTATCAAGGTTAATGGTAAAGAGGTTTTAATAAATAATCCAAATGATGCTAATAATTTGGGTATTGGTATGGTTCACCAACATTTTAAATTGGTTGGTGCATACACAAACTTACAAAATATAATTCTTGGCGCTGAAAGTGAATCAAAAACTTTTAAAACTTTGGATTATAAACCATCAATTAAAAAAATTAAAACTATTCAAAGATTATTTAATTTAAATTTTGATTTGAATAAAGACACAGGCACTGAATCAGTTTCAACACAGCAAAAAGTTGAAATCATGAAGATGTTATATCGTGATTCAGAAATTCTAATCTTTGATGAACCAACTGCTGTTTTAACAGATGAAGAAATTCAAGGTCTTCTTGAAACATTCAAATTGTTTAAATCGCAAGGTAAAACAATCATTTTTATCTCTCACAAACTAAAAGAAGTTAAAGCTGTAGCTGATTATGCAACAGTTATACGTCACGGTAAAGTTACTGGTAACTTCGAAGTTGCTAATACTTCTATTGAAGAAATGGCTAACAGAATGGTTGGTGACAATATTGTTATGGCACAAAACCAATCACTTGACGTTTTTGAAGATAAAGACACAGTTTTAGAACTTGAAAATGTATCAACAAAAGGTACAAAATCACTTAAAAACTTAAATCTTAAGGTTCATGCTGGAGAAATTCTAGCTATTGCTGGTGTTGAAGGCAATGGACAACAAGATTTAGAATATGTTGTTAGTGGAATTGTTAAACCTACAAAAGGTAATGTCTACTTAAAGAAAACACCTTTAGTTGAGGAAAGAGTTAAATTCATTCATAAAAATAAAATCAAGAATTTCATTATTTTAAGTTCATTGCTTGCATTATTTATAGCTTTTGGATCAATTTTTTTTGGTGTTAGTCCATTTCAGCAAAATTATTCAAACATTTGA", "aa": "MNAIEFINISKSFGVIKANQDINIQIKKGTIHALIGENGAGKSTLLSILFGLYEPDHGIIKVNGKEVLINNPNDANNLGIGMVHQHFKLVGAYTNLQNIILGAESESKTFKTLDYKPSIKKIKTIQRLFNLNFDLNKDTGTESVSTQQKVEIMKMLYRDSEILIFDEPTAVLTDEEIQGLLETFKLFKSQGKTIIFISHKLKEVKAVADYATVIRHGKVTGNFEVANTSIEEMANRMVGDNIVMAQNQSLDVFEDKDTVLELENVSTKGTKSLKNLNLKVHAGEILAIAGVEGNGQQDLEYVVSGIVKPTKGNVYLKKTPLVEERVKFIHKNKIKNFIILSSLLALFIAFGSIFFGVSPFQQNYSNI", "aa_hexdigest": "e108c33640f696e8ee45810ad4a72326", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00111B63FE", "query_cov": 0.9700272479564033, "subject_cov": 0.9246753246753247, "identity": 0.64, "score": 436.0, "evalue": 1.72e-149, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A223M8X7" }, "pscc": { "uniref50_id": "UniRef50_A0A223M8X7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "IGIGPGHMMC_1543", "locus": "GCA000148625_01538" }, { "type": "cds", "sequence": "contig_1", "start": 881212, "stop": 881406, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAAAAATTATTAAATTCTTTAAAACCAAAAAAGATGAAGAGAAACTTAAAGACAGGATTAATTTAAACAATTATTCAGTCTTAGAAAAATCAATGCTTGCTTCAAGTTTTATTCCAAGTGATAGACATAAACATGGTCTAGCATTAGATTACAATATTAAAAATAATACAATATTAAGACGTTTATGA", "aa": "MIVKIIKFFKTKKDEEKLKDRINLNNYSVLEKSMLASSFIPSDRHKHGLALDYNIKNNTILRRL", "aa_hexdigest": "a620a6519f26e53850423a242e50ae21", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7590.869299999998, "isoelectric_point": 9.994485282897951 }, "id": "IGIGPGHMMC_1544", "locus": "GCA000148625_01539" }, { "type": "cds", "sequence": "contig_1", "start": 881569, "stop": 881853, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCACCACACGACTTTATATTAATTCTTCAACCAACACGTGGACTTGACGTTGGGGCTATTAAAAATATCCATGAAAGAATTCTTAAAGAAAAAGCAGAAGGAAAAGCAATTCTTTTAATTTCTTATGAATTAGACGAGGTTCTTTCACTAGCTGATACTATTGCTGTTATTAACGAAGGCGAAATTAGACTTTCAAAACCCGCAAGAGAATTAACTAGAAAAGAAATCGGTCTTTACATGGCTCAAAAAGAACATCAAACGGAAGGAGGCACACAATAA", "aa": "MNSPHDFILILQPTRGLDVGAIKNIHERILKEKAEGKAILLISYELDEVLSLADTIAVINEGEIRLSKPARELTRKEIGLYMAQKEHQTEGGTQ", "aa_hexdigest": "0f4e1c1824615bf988e90214beb30225", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10521.004699999992, "isoelectric_point": 5.577576255798339 }, "id": "IGIGPGHMMC_1545", "locus": "GCA000148625_01540" }, { "type": "cds", "sequence": "contig_1", "start": 882444, "stop": 882746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATCCAGAAGCATATTCACCTACAACTATAGGTAGTGCTAGAATTGCAATTGAAAATGCAGTTAAATATCAATTTGTTTACTTTGGTATTGGAATGTTTGTTTTAACAGCTATTGTTTTATTTGTGATCTACAACTTCACAACATTAGGTTACAAAATAAACATGGTTGGTAAAAATCCTTCAAACGCTAAATATATTGGTCTTAACGAAAAAATGTTAACCATTATAGTTATGGGATTTTCAGGAGCTTTAGCTGGTTTCGCTGGATTCTACTACATTATTATCAAAGAAGGCTGA", "aa": "MFNPEAYSPTTIGSARIAIENAVKYQFVYFGIGMFVLTAIVLFVIYNFTTLGYKINMVGKNPSNAKYIGLNEKMLTIIVMGFSGALAGFAGFYYIIIKEG", "aa_hexdigest": "72d2ecaf49a7fe70383fab20ffe32956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11029.922999999993, "isoelectric_point": 9.222927665710447 }, "id": "IGIGPGHMMC_1546", "locus": "GCA000148625_01541" }, { "type": "cds", "sequence": "contig_1", "start": 882861, "stop": 883001, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACAACAAGAGATTTATTTGCTATAAAAGATAAATTAACAAAAGACTTTTTCCCTATTATTACTGGTATAATTGTCTTTATGGCTGCTTTAGCTATTATGCTTTATAAATTCAAACCACTTGGTTTCATGTGA", "aa": "MIYTTRDLFAIKDKLTKDFFPIITGIIVFMAALAIMLYKFKPLGFM", "aa_hexdigest": "94e0e6c73b4c18eca007b21badbcf0dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5348.580299999999, "isoelectric_point": 9.601614952087402 }, "id": "IGIGPGHMMC_1547", "locus": "GCA000148625_01542" }, { "type": "cds", "sequence": "contig_1", "start": 883056, "stop": 883172, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCAAAAATGCTAAATATAGAGAATATGTTAAAGCTCTTAATGAAGAAAAAAGAAGGAATAGAAAACTCAAAAAAGAATTTGCTTCAATTAAAGAAGAATATCAAAAATGA", "aa": "MKTKNAKYREYVKALNEEKRRNRKLKKEFASIKEEYQK", "aa_hexdigest": "a34dee78871f7ca0cfabb5913749dc8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4777.5102000000015, "isoelectric_point": 10.060952186584473 }, "id": "IGIGPGHMMC_1548", "locus": "GCA000148625_01543" }, { "type": "cds", "sequence": "contig_1", "start": 883221, "stop": 883388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATCTATGACGAAATGTCTAAGAAAAAATTCGAATATCTCAAAAAATACAGTGATTTTGGCTTAAACAATCTTAAAACATTAAAAAATCAATATAAAGCTGAAAAACACGAAAGAAAAATTCAATATATTACTAAAAGAGATTTAATTTATACATCTCATTGA", "aa": "MSIYDEMSKKKFEYLKKYSDFGLNNLKTLKNQYKAEKHERKIQYITKRDLIYTSH", "aa_hexdigest": "f2d3fd8a02e7960c808e320debbeaf2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6808.792300000001, "isoelectric_point": 9.619150352478027 }, "id": "IGIGPGHMMC_1549", "locus": "GCA000148625_01544" }, { "type": "cds", "sequence": "contig_1", "start": 883502, "stop": 883669, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGCTCTAACACTTGCAACTCTTTCAGGTATTTTTTCAGAAAGAGTTGGTATTACAAACATTGCTGTTAACGGGTTCATGATTTTTGGAGCTGCTATGTATGCTCTAATAAGCTTCCTAATGACTGATGTAATTTTCAAAGGTAAAACATTAAGTCCATGA", "aa": "MFSALTLATLSGIFSERVGITNIAVNGFMIFGAAMYALISFLMTDVIFKGKTLSP", "aa_hexdigest": "55b291549bccb2bccf22deb32b6639f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5878.9815, "isoelectric_point": 8.253774452209473 }, "id": "IGIGPGHMMC_1550", "locus": "GCA000148625_01545" }, { "type": "cds", "sequence": "contig_1", "start": 883724, "stop": 883909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTTTTGCAACGATTAAACTTAAATCAGATCAATCTGTTTCAGGTTTCGCTATGAACATTCTTGCAGTTGGTGTTGCTTCTATCCTTTTATTATTTGTTAAAAAAGCACAACAAGGTGGAGACTACTTCCAAATGCGTGGCCAAACAGAACTTGCTTTAAGTCCTAATGTTGACAACTGA", "aa": "MFGFATIKLKSDQSVSGFAMNILAVGVASILLLFVKKAQQGGDYFQMRGQTELALSPNVDN", "aa_hexdigest": "d8313d467a952afb41aa5abb773ef524", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6567.566100000003, "isoelectric_point": 8.139343070983887 }, "id": "IGIGPGHMMC_1551", "locus": "GCA000148625_01546" }, { "type": "cds", "sequence": "contig_1", "start": 884303, "stop": 884443, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTGTTTCAAGATTCGCTGAACTACTCTACACATTGCCATTTATTATCACACTTGGAATTATGATTGCTAAAATGAAAAGCAAGCAAATGGGGCCAGCCGCTGCAGGTCGACCTTATGACAAATCAGAGCGTTAG", "aa": "MKGVSRFAELLYTLPFIITLGIMIAKMKSKQMGPAAAGRPYDKSER", "aa_hexdigest": "dd7652daf9c1160972968470b421fec8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5117.128300000002, "isoelectric_point": 10.11536350250244 }, "id": "IGIGPGHMMC_1552", "locus": "GCA000148625_01547" }, { "type": "cds", "sequence": "contig_1", "start": 884462, "stop": 886504, "strand": "+", "frame": 2, "gene": null, "product": "Phase variable surface lipoprotein P78", "db_xrefs": [ "RefSeq:WP_013354995.1", "SO:0001217", "UniParc:UPI0001E33043", "UniRef:UniRef100_UPI0001E33043", "UniRef:UniRef50_O06764", "UniRef:UniRef90_O06764" ], "nt": "ATGAAAAAAAGAAAATTTATATTTGCTTTATCATCATTAACAACAGCTTTTTTAGCTGCACCTCTTATTTCTTCATCATGTACTAATGCTGATAAATTGTCTAATCTATTAGATGATTTAGACATTACTATTCCAGATTCAGAATCTAAAAAACCAAATGAAATCAAAGAATCGGATTTAAAATTTTACTCAAAAAATATGCCAAGTTTTAATAGTGAATATACTGTTGAAATACAAAAAATTCAAAAATATTCTTCAGAACTTGTAGTAACTTTTAGACTACGCGAAAAAAACTCAAACATTGTTTCAAAATACAAAACAATTACATACAAAAATTTCTTAAACTTTGATCATATTAATGAAGCTTTAACAGTACAATATTCTAATTCAAAAAATACTTATGTAAAAGATGTTGCTCAAACAAGTGACATTATCTTTAGAGATATTAAAACAGGATACAAAATTATCGATGTTAAAATTGATAACTCAAGAATTGAAGAATATCAAAAAAATTTATCTGACAAACTAAAAGATATAAATGAATTCTTAACAAAAAATAAATCTGAATATGATGCTGAAAAAGCTAAAGCAAATCCTAATAAGGAAATTATTGAAAAATATGAACGCGCTTTAATTAGAAAAGCTAAATATGAAGATACTGCGGATAAAGATTCATATATCAAATCATTCGAGAATTTAAATATAAAATACAAAATTCAATCAATAAATGATCCTAAAATTGTATCTGATGAATTCAGTCGTTCAATTCCAAACTTCAAAAATGATTCATACATTGATTACTATGTTTTTGATAAAAGTGACACATACTCAACAATTTCGCATTCATTTCACTCTGTTTCAGATTTAACTGAAGAAATAAAGAAATACATTAAAAAAACTGCTTCACTAGAATATACAGGAAATACAAATGTTTCTGTATCTGAAGTTAAGGCTGATAAATTTAAATTTAATTTTGACACACCAATTGATTTTTCAATAGAAAACATCAAAGTTGAACCTGCTGATTCAGAAGGCAAAGTAAATCTTAAATTTAATATTAAAGCACCTATTAACAAACAAGCAACAACTTTATTAGTTGATGAAAACAAAAAAACAATTGAATTTGATTATGCATGTGAAATAGAATTTAACAAAAAAGATAGTAAAGAAAAATTAAGTGAAATTGAAAAACAAATTAGCTTTACATATGAAAATGCTGCAACAACAGAAGTTGATAAGGCAGAAGTTTCTAAAGTTAAATACACTTTATCTAACCCAAATTATGAAATTACTGATTTAAAAATTATTGGTTATGAATTCAACAATTCAACTATTGTTATTGAATACAAAGCAAAAAGTAAAAGCAGTTCAATTGAACAAGTTTATTCAAGAACAATTAGTGGCTTTTCTAAACTTTCACAATCTAAAATTGATGCAATTTTAGATTCAATTAAATTGAATTATAAACGTAAAAACTTAGTAGCTGCAAATGAAGCGAATAAAGATCATGTATTTATTGAATCAATGGATTCAAACTTCATGATTGAAGTTACAAAAATAGATTCATTTGATCTTAAAGATAAATCAATATTAGTTGAATTTAAATTATATGAAGTTGATGCACTTTCTAAATTAAAACCAGACAACAAAAAACGCGGTTTCAAATTTGTTAAATCATTCACAAAGAAAATTACAGGCTTCTCAGAAAAATCATTGACACAACATATCAATGAAGAAATTCAAAAGGTCACTTGTACTTGTCCAACAGCTTCAAGCAAATTGCCAAGTCAATTAAACATTAATAATATTAAATTTACTAACTTTGATAACAATATTTTTGAAATTGAAAAACCATCAATAATTGTTGCTAATGATCAAAAAGGCGAAGTTGTTATTACATTTAAATTACGTTACAAAATTGGTTATGTACAAAAAGTTTATGACCAACAAAAGAATGAATATGTTGAAAAAGAAATACCTTTCAAAACTGTTTCAGACTCAAAAACAGTTATATTAAATGGTTTTAAAAAAAATAAATTATAA", "aa": "MKKRKFIFALSSLTTAFLAAPLISSSCTNADKLSNLLDDLDITIPDSESKKPNEIKESDLKFYSKNMPSFNSEYTVEIQKIQKYSSELVVTFRLREKNSNIVSKYKTITYKNFLNFDHINEALTVQYSNSKNTYVKDVAQTSDIIFRDIKTGYKIIDVKIDNSRIEEYQKNLSDKLKDINEFLTKNKSEYDAEKAKANPNKEIIEKYERALIRKAKYEDTADKDSYIKSFENLNIKYKIQSINDPKIVSDEFSRSIPNFKNDSYIDYYVFDKSDTYSTISHSFHSVSDLTEEIKKYIKKTASLEYTGNTNVSVSEVKADKFKFNFDTPIDFSIENIKVEPADSEGKVNLKFNIKAPINKQATTLLVDENKKTIEFDYACEIEFNKKDSKEKLSEIEKQISFTYENAATTEVDKAEVSKVKYTLSNPNYEITDLKIIGYEFNNSTIVIEYKAKSKSSSIEQVYSRTISGFSKLSQSKIDAILDSIKLNYKRKNLVAANEANKDHVFIESMDSNFMIEVTKIDSFDLKDKSILVEFKLYEVDALSKLKPDNKKRGFKFVKSFTKKITGFSEKSLTQHINEEIQKVTCTCPTASSKLPSQLNINNIKFTNFDNNIFEIEKPSIIVANDQKGEVVITFKLRYKIGYVQKVYDQQKNEYVEKEIPFKTVSDSKTVILNGFKKNKL", "aa_hexdigest": "33118b22c7e45db8f0e958761df0743d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33043", "ncbi_nrp_id": "WP_013354995.1", "uniref100_id": "UniRef100_UPI0001E33043", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33043", "RefSeq:WP_013354995.1", "UniRef:UniRef100_UPI0001E33043" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33043", "uniref90_id": "UniRef90_O06764" }, "psc": { "uniref90_id": "UniRef90_O06764", "product": "Phase variable surface lipoprotein P78", "uniref50_id": "UniRef50_O06764" }, "pscc": { "uniref50_id": "UniRef50_O06764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O06764" ], "product": "Phase variable surface lipoprotein P78" }, "genes": [], "id": "IGIGPGHMMC_1553", "locus": "GCA000148625_01548" }, { "type": "cds", "sequence": "contig_1", "start": 886849, "stop": 886938, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGAAATTAATCAAGATGAATACAAGAACAATGTTATTGGAAAAGAAAAAGGCATTTATGTTTTGGTTTTTCACGCATTATGA", "aa": "MLKEINQDEYKNNVIGKEKGIYVLVFHAL", "aa_hexdigest": "fbeb342cc3e90903ea2cbb81877de32e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3406.9455000000007, "isoelectric_point": 6.523149681091309 }, "id": "IGIGPGHMMC_1554", "locus": "GCA000148625_01549" }, { "type": "cds", "sequence": "contig_1", "start": 886954, "stop": 887073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCTTCTTTAGAACAATTAGACAAATTAGACAACATTCCAGTTTATCGTGTAGATGTTGATGAAAATACAAAGCTAACTCAAGAAATGGGTGTTAATTCAATGCCAACATGA", "aa": "MFKSSLEQLDKLDNIPVYRVDVDENTKLTQEMGVNSMPT", "aa_hexdigest": "444ad36c6f5862ce8e726c07b9f6df7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4487.048000000001, "isoelectric_point": 4.385492134094237 }, "id": "IGIGPGHMMC_1555", "locus": "GCA000148625_01550" }, { "type": "cds", "sequence": "contig_1", "start": 887400, "stop": 887660, "strand": "+", "frame": 3, "gene": "ssrA", "product": "SsrA RNA (TmRNA)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "nt": "ATGTTAGTTAAGTGTGATGAAACTAGAGATCGCAAATTATTGATGCATAAAAATGAAATAGTTAGATTGCAGTCTAAACTTCATAAGCTAGGTCATGCAACTATTATTCCAAGCAAAATTTATTTTGATAATCGCTCACGTATTAAGATTGAAATAGCTTTAGTTCAAGGAATGAAAAAAACAGATAAACGTGAAGAAATCAAAAAAAGAGATAATGAAAGATACATCAAAAAAAGTCTTGAAAAATGTATATTAAAATGA", "aa": "MLVKCDETRDRKLLMHKNEIVRLQSKLHKLGHATIIPSKIYFDNRSRIKIEIALVQGMKKTDKREEIKKRDNERYIKKSLEKCILK", "aa_hexdigest": "afa02fdc2e0d54bca4e424003d802636", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P825", "query_cov": 0.9302325581395349, "subject_cov": 0.8791208791208791, "identity": 0.5, "score": 77.8, "evalue": 4.92e-17, "valid": false, "product": "SsrA RNA (TmRNA)-binding protein", "uniref50_id": "UniRef50_A0A3B0P825", "kegg_orthology_id": "K03664" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "product": "SsrA RNA (TmRNA)-binding protein" }, "genes": [ "ssrA" ], "id": "IGIGPGHMMC_1556", "locus": "GCA000148625_01551" }, { "type": "cds", "sequence": "contig_1", "start": 888173, "stop": 888292, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAGTTTTCTTTCTTCCTGAAGATCCTCAAAACACAATTACAAACTATATTAAGAACACAAATTTTGAATCAAATATTTTAGTTTATGGCAAAGAAGTAGAAACATTAAATTGA", "aa": "MAIVFFLPEDPQNTITNYIKNTNFESNILVYGKEVETLN", "aa_hexdigest": "22f8da0404af7745050b8ab6046c161f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4521.060600000001, "isoelectric_point": 4.251295280456543 }, "id": "IGIGPGHMMC_1557", "locus": "GCA000148625_01552" }, { "type": "cds", "sequence": "contig_1", "start": 888299, "stop": 888409, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGAATTGAACAAGGAGCAAAGACAAGAAATAGAAAAGTTGTTATAACTCACTCTTCACCTAATCCTGAAGAATATATTGAAACTATCAAATATGTTTCAAGCTGA", "aa": "MNGIEQGAKTRNRKVVITHSSPNPEEYIETIKYVSS", "aa_hexdigest": "cf9bdc96af1bf43440c5738923bdd480", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4077.5335000000014, "isoelectric_point": 8.161713600158691 }, "id": "IGIGPGHMMC_1558", "locus": "GCA000148625_01553" }, { "type": "cds", "sequence": "contig_1", "start": 888434, "stop": 888640, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00356.27" ], "nt": "ATGAAAAATTTCTCATATAAAAATATTGCTAAAGAAGCAGGTGTCTCTATTAGTACTGTAAGCCGTTACTATCGTGGTGGTTATGTTAGCAAGGAAACTAAAAAAGTTATTGCTAATATCATAAAAGAAAACGATTATTATCCAAATCATGGAGCTAGAACAATTCGTGGCCGTGACACTTCTATCTTTATTATTGTGCCTGAGTGA", "aa": "MKNFSYKNIAKEAGVSISTVSRYYRGGYVSKETKKVIANIIKENDYYPNHGARTIRGRDTSIFIIVPE", "aa_hexdigest": "ec7b210ac875650da08381d424306d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00356.27", "name": "LacI", "length": 44, "aa_cov": 0.6323529411764706, "hmm_cov": 1.0, "evalue": 4.2332111208457344e-15, "score": 51.34090042114258, "start": 7, "stop": 49 } ], "seq_stats": { "molecular_weight": 7759.767100000002, "isoelectric_point": 9.791796684265137 }, "id": "IGIGPGHMMC_1559", "locus": "GCA000148625_01554" }, { "type": "tRNA", "sequence": "contig_1", "start": 889095, "stop": 889168, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 66.5, "nt": "GCGAATGTAATTTAATGGTAAAATGCCAGCTTGCCATGCTGATAATGGGGGTTCGATTCCCCTCGTTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 889119, 889121 ], "id": "IGIGPGHMMC_15", "locus": "GCA000148625_01555" }, { "type": "cds", "sequence": "contig_1", "start": 889675, "stop": 890379, "strand": "-", "frame": 2, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "nt": "ATGGCTGTTAACAAAGTGTTTTCAGATTCATTTGAACGTTTCTTAAATGAAAATCCTGCTGAAGCAAAAACTATTGTTCAAAAAACAATTTTTGCTAAAAAATCTCGTCTTGCTGGTTTAGCAGCTAGAGATGCAGCTAGAAGAAAAACTGTTTTTGATACAGGTTCACTTCCAGGAAAATTAGCTGACTGTTCTTCAAAAAATGCTGAAATTAGTGAGCTTTACATTGTCGAAGGTAACTCAGCTGGTGGTTCAGCTAAAATGGGAAGAAACAGAATGACTCAAGCTATCTTGCCTCTTCGTGGTAAAGTTATTAATGCTGAAAAGAATCAACCTAAAAAAGTGTTTGCAAATGCTGAAATTCTTTCATTAATTACAGCACTTGGAACAGGTGTCGGTGAAGAATTTAATATTAATAAATTAAGATATCACAAAATTATCATCATGACCGATGCCGATGTCGATGGTGCTCACATTAGAACATTGTTATTGACGTTCTTCTACCGTTACTTTAGACCTTTAATTGAATACGGATTTATTTATATTGCTCAACCTCCTTTATATAAAATAGCTCAAAACAAATTTATTGGATATGCTTACAACGATGCTCAAAAAGAAGAAATTATGCAAGGCTTAAATCAAAATCAAAAAGTAACAGTTCAACGTTACAAAGGACTTGGTGAAATGGACCCTGAACAACTTTGA", "aa": "MAVNKVFSDSFERFLNENPAEAKTIVQKTIFAKKSRLAGLAARDAARRKTVFDTGSLPGKLADCSSKNAEISELYIVEGNSAGGSAKMGRNRMTQAILPLRGKVINAEKNQPKKVFANAEILSLITALGTGVGEEFNINKLRYHKIIIMTDADVDGAHIRTLLLTFFYRYFRPLIEYGFIYIAQPPLYKIAQNKFIGYAYNDAQKEEIMQGLNQNQKVTVQRYKGLGEMDPEQL", "aa_hexdigest": "b0dd183ac76f8b59f866f16a6b0b59f4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A380DNC1", "query_cov": 0.9914529914529915, "subject_cov": 0.8083623693379791, "identity": 0.586, "score": 276.0, "evalue": 1.96e-90, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A380DNC1" }, "pscc": { "uniref50_id": "UniRef50_A0A380DNC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "IGIGPGHMMC_1560", "locus": "GCA000148625_01556" }, { "type": "cds", "sequence": "contig_1", "start": 891040, "stop": 891285, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGGCAGGTTTTGCAAACAAAGTAGAAATCACGATTACAAAGGATGGTTACATTGATGTTATCGATAATGGTCGTGGTATTCCTACAGCAGTTCACCCTAAAACTGGTCTTTCAACAGTTGAAACAGTTTTAACGGTTTTACATGCTGGCGGTAAGTTTGACTCTGACTCATATAAAGTTTCTGGTGGACTTCACGGGGTTGGTGCTTCAGTAGTTAATGGACTTAGCGATGACTTTGAAGTTTGA", "aa": "MAGFANKVEITITKDGYIDVIDNGRGIPTAVHPKTGLSTVETVLTVLHAGGKFDSDSYKVSGGLHGVGASVVNGLSDDFEV", "aa_hexdigest": "cdea0663282a34fd64db0bcb153c438c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 1.0, "subject_cov": 0.84375, "identity": 0.58, "score": 102.0, "evalue": 6e-27, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 77, "aa_cov": 0.9135802469135802, "hmm_cov": 0.8181818181818182, "evalue": 1.3765809027919204e-11, "score": 40.88747787475586, "start": 4, "stop": 77 } ], "seq_stats": { "molecular_weight": 8347.249399999999, "isoelectric_point": 5.032149314880371 }, "id": "IGIGPGHMMC_1561", "locus": "GCA000148625_01557" }, { "type": "cds", "sequence": "contig_1", "start": 891316, "stop": 891435, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAAGAATATGATGCATCGAATATTAAAGTTTTAGAAGGATTAGAAGCCGTTAGAGTTAGACCTGGTATGTACATCGGTACAACAGGAATTAGAGGTCTTCACCACCTTATTTGA", "aa": "MSKEYDASNIKVLEGLEAVRVRPGMYIGTTGIRGLHHLI", "aa_hexdigest": "a62aa19c0147e26c6a8c113928cd8d31", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4326.009699999999, "isoelectric_point": 8.230243492126466 }, "id": "IGIGPGHMMC_1562", "locus": "GCA000148625_01558" }, { "type": "cds", "sequence": "contig_1", "start": 891772, "stop": 892074, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAATAAATCACAAAATGATGAAAAATTAAATCAATCATCAGTCGAAGAACATAATGTAAATGAAGAACATACACAAAAACAAAAAGACAAAGAAGTGATGGACGAGGTTAATGCATTCGAAAGAGACGTTTTACTTAATAATGGTAGAGTCGAAGACAAGCATGTTGAAGAACATCATGAAATGAATTCAATTCATTTAGATGACGTTGTTAACAAAAAAAATACTCAATACAAATACACAAAAATGTCGAATTTTGTGTTGAAATTATCTCGTTTTTATGCTCCTATGCCTCTTTGA", "aa": "MPNKSQNDEKLNQSSVEEHNVNEEHTQKQKDKEVMDEVNAFERDVLLNNGRVEDKHVEEHHEMNSIHLDDVVNKKNTQYKYTKMSNFVLKLSRFYAPMPL", "aa_hexdigest": "add831090532a0044360f9a2be48465e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11884.080499999991, "isoelectric_point": 5.637370872497558 }, "id": "IGIGPGHMMC_1563", "locus": "GCA000148625_01559" }, { "type": "cds", "sequence": "contig_1", "start": 892588, "stop": 892812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTTTCTTCTTTGCAATTATTGCAATAATAGGAGGTAGTGCAGGAGTTACTGGAATTATTGGCGAATATATGGCTGTCGTTAAACAAAAAAACTTTGGAACAATTAATGGTTATATCAACTTAGTAATTATTATTGTTTCAGTTGTACTTGGTACATACTTACCAGGAAGTTTATTATTAAATGACATTAATGGTTTTAAAGCAGATTCAGTGACTTGA", "aa": "MLAFFFAIIAIIGGSAGVTGIIGEYMAVVKQKNFGTINGYINLVIIIVSVVLGTYLPGSLLLNDINGFKADSVT", "aa_hexdigest": "8e8453df4bfebabf6b9316a0efcba96b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7738.106599999996, "isoelectric_point": 5.875696754455566 }, "id": "IGIGPGHMMC_1564", "locus": "GCA000148625_01560" }, { "type": "cds", "sequence": "contig_1", "start": 892936, "stop": 893238, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTACCTTAACAAATTATTCCCTCGTTACAAAATTGTTCAAGTTAAAATTTACTCGCCTCACATGAGTGCTATTAGAGAAGCAATTATTAGTGATAATAAAACTGTTAATAGCTTTACTATCACTAAAGGCGTTGGAGGTTACTCAGGAAATAACACCAAAGTTCTTTCATCAATTACTCTTTACAATCAAGTGCCACGTCTTATTAAGAAAGTTCGGGCCGTTGATCAAAACTCGCTGATTACTATTAGTAATGTTGCTAGTGTTGATGGAAAAATATATCTTCCTGAAAACAAATTCTAA", "aa": "MYLNKLFPRYKIVQVKIYSPHMSAIREAIISDNKTVNSFTITKGVGGYSGNNTKVLSSITLYNQVPRLIKKVRAVDQNSLITISNVASVDGKIYLPENKF", "aa_hexdigest": "b27be12c95e6d88241986559dd2e78de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 53, "aa_cov": 0.53, "hmm_cov": 1.0, "evalue": 6.363319330907661e-14, "score": 47.618621826171875, "start": 39, "stop": 91 } ], "seq_stats": { "molecular_weight": 11222.946299999989, "isoelectric_point": 9.99583911895752 }, "id": "IGIGPGHMMC_1565", "locus": "GCA000148625_01561" }, { "type": "cds", "sequence": "contig_1", "start": 893305, "stop": 893412, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAACTATATTGAATACAATGGCGACAAAGTTCCTTTATATATAGAAGATAACAAAAAAAATACAACTTTTTTGTTTCTTCATGGACTTAATTCAAACTGA", "aa": "MKLNYIEYNGDKVPLYIEDNKKNTTFLFLHGLNSN", "aa_hexdigest": "2a8e932a89320bde0eb10530101fc8ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4147.662500000001, "isoelectric_point": 6.517181587219238 }, "id": "IGIGPGHMMC_1566", "locus": "GCA000148625_01562" }, { "type": "cds", "sequence": "contig_1", "start": 893448, "stop": 893582, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCACTTTTTTAGTTTTAATTCTATTTAAAACCTCTTTAGCTACTTCAATTCATCACTCAAGTTTAATTTCTTCTGGTTCTACATTTTTGAAAAATTTACTTCCTGGAAAATTAATAGCCACAACATTGTAA", "aa": "MTTFLVLILFKTSLATSIHHSSLISSGSTFLKNLLPGKLIATTL", "aa_hexdigest": "07f256863019d40f7c953417b06d93ed", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4717.610199999998, "isoelectric_point": 10.301419258117672 }, "id": "IGIGPGHMMC_1567", "locus": "GCA000148625_01563" }, { "type": "cds", "sequence": "contig_1", "start": 893596, "stop": 893736, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGGTGGCGTGGCTACTGTTTTAGCTAAGGATCCTAAAGTTGTTAGATTAATTATGATGTCAACAATTAATCCTTATATGAAAGAATCTACTTCTTATTCAGTGTTACAATCTGTTATTAAACCTCAAAATAAATGA", "aa": "MAGGVATVLAKDPKVVRLIMMSTINPYMKESTSYSVLQSVIKPQNK", "aa_hexdigest": "9eb0c225c598cdb542dc8fee2cfafe2d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5025.969600000002, "isoelectric_point": 9.873542594909669 }, "id": "IGIGPGHMMC_1568", "locus": "GCA000148625_01564" }, { "type": "cds", "sequence": "contig_1", "start": 893869, "stop": 894093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAATTAGATTTACAATACAAAGCAAATTGTCACAAAATGCTCTTTTTAGTAGGTGAAAAGGACAAAATTATTGGTACTAAATATTTTGAAGATTATGCAATTAGTTTAGAAGTTCCAATTTTAATTATGGGTACGGGACACTCGCCAATTAAAGATGAACCAAAAAGAATTGCAAGATTTTTTAATGCATTGATTGAAAGCAAAAAAAGACACTTTTGA", "aa": "MEQLDLQYKANCHKMLFLVGEKDKIIGTKYFEDYAISLEVPILIMGTGHSPIKDEPKRIARFFNALIESKKRHF", "aa_hexdigest": "be5977e579ddc5690897837d38251f35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8656.085799999993, "isoelectric_point": 8.761269569396973 }, "id": "IGIGPGHMMC_1569", "locus": "GCA000148625_01565" }, { "type": "cds", "sequence": "contig_1", "start": 894103, "stop": 894195, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAAAATGAATCAATTCTTAGAATTTGATGAAGAAAAAACAAAAGAAGAAGATATTCTTCTTGAACTAGAACAAAACGTTGATTAG", "aa": "MNIKMNQFLEFDEEKTKEEDILLELEQNVD", "aa_hexdigest": "a270d6dae8cbc8a6f02061a52e16896c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3686.078700000001, "isoelectric_point": 4.09805736541748 }, "id": "IGIGPGHMMC_1570", "locus": "GCA000148625_01566" }, { "type": "cds", "sequence": "contig_1", "start": 894205, "stop": 894696, "strand": "+", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XFU3" ], "nt": "ATGGAATACAATCAATTTGAAATGGAACTTAGAAGTGCTTTAACAACAGGCAATGGTACAAAACTTTTATACAAAATAATTGATGCACCTTATAGATTTTTTTCACCTTTGCAACCTTTTAATTACAAAGTTAAATTAGAACAAGCATTCTTAAGAACACAAGAAAACACATATGTAAAATTTATTAAATATTGTGCTGAATATTTAATTACTCAATATGGTTTTAAATTAGAAAAAAGTTCTTTTAAATATACTTTACCACTTAAAGAGGGAGAAGAATTCCCAGAAGAAGTAAAAGTCAATGCAGCTATGATTTTTAGACAAAAAGAAAATGGCGGAGATGAAGAAACAGCTGATATTTGTGTAATTTACTTGAAAAAAAGAGATTACTATGGAGCTAAAGAAGTTACTAAACTTCATGACAATATCATTAAGCAATTAGAAGTATTAAGAATAATTTATCCTAATAATAAAATCAAAGGTATCTTATGA", "aa": "MEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGIL", "aa_hexdigest": "811897c888de565fa2d70d057c393c28", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 894199, "stop": 894999, "observations": { "mutation": [ 894694, 894832, 894958 ], "stop": [ 894694, 894832, 894958 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU3", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 1.0, "score": 323.0, "evalue": 1.25e-109, "gene_start": 1, "gene_end": 163, "reference_start": 3, "reference_end": 165, "reference_sequence": "MIMEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGILWFLDNDYHKNVNFFNECYDKEHSFETPGFRAYYGAQFFKIFDKEEDWLLVENHIKKFKNSNYDYFLKMPDLDQDEDVLNAMINLNETSWQKLISPDEVYANIRQYIFNERNENSNLFKALKLRDIKRTSVDDDEEKRRILELENKANQNE" }, "paralog": false, "identity": 0.9849624060150376, "score": 533.0, "evalue": 6.28e-195, "description": "Internal stop codon at 894694, 894832, 894958. Nonsense mutation around 894694, 894832, 894958.", "psc": { "uniref90_id": "UniRef90_C4XFU3", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU3", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "IGIGPGHMMC_1571", "locus": "GCA000148625_01567" }, { "type": "cds", "sequence": "contig_1", "start": 895223, "stop": 895477, "strand": "-", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "nt": "TTGCCTTCAGAAATTAAGTATCGTGAAATATCATCAGTTTCATATATGGGAGATTTCCAAGCACGTCGTGCAATGATTAGATATAAGAATGAAAATGGTGAAAATGTTTATGCTCATACCATGAATGGTTCAGGTCTTGCAATTGATAGATTAATTGCAGCAATTCTCGAAAATTATCAAAACAAAGATGGTTCAATTACTATACCTAAGAAATTAGTTCCTTATATGGACGGAATAACAAAAATTACAAAATAA", "aa": "MPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK", "aa_hexdigest": "5f51a468b16bf6f300185d105ab8bf5c", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI00387B1930", "query_cov": 0.9404761904761905, "subject_cov": 1.0, "identity": 0.696, "score": 112.0, "evalue": 9.2e-31, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI00387B1930", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B1930", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "IGIGPGHMMC_1572", "locus": "GCA000148625_01568" }, { "type": "cds", "sequence": "contig_1", "start": 895478, "stop": 895594, "strand": "-", "frame": 1, "gene": null, "product": "Seryl-tRNA(Ser/Sec) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "nt": "ATGCTTGAACAAGCTAAATCGGTTTTAGAAGAATTAGAAATACCTTATAGAGAATTATTATTATGTACAGGTGACTTAGGATTCTCAAGTAGAAAAACTATTGACTTAGAAGTTTGA", "aa": "MLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEV", "aa_hexdigest": "93f8c13faf2742f3e7b9ef82e59c43b7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920HIT7", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.579, "score": 48.9, "evalue": 6.19e-07, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_A0A920HIT7" }, "pscc": { "uniref50_id": "UniRef50_A0A920HIT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "product": "Seryl-tRNA(Ser/Sec) synthetase" }, "genes": [], "id": "IGIGPGHMMC_1573", "locus": "GCA000148625_01569" }, { "type": "cds", "sequence": "contig_1", "start": 895613, "stop": 896494, "strand": "-", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q6KIJ8", "UniRef:UniRef90_C4XFU2" ], "nt": "ATGCTAAATTTAAAATACGTTTTAGAAAATGAAAAGAAAGTTAAAGAAGCTTTAAAAAATAGAAATTTCGATCTAAAGCAATTTAATGAGCTTATTAAAGAAGCAAATAAGCGTGGAAAGCTAATGTTTGAAGCTCAAAGCAAGAAAGCTGAATTAACAAAATTTAGTAAAGAATTTGCAGCTAACAAAAACAATAAAACTAAATTAGCTAAATTACAAAAAGAAGTAGCAAATATTAAAAAAGAACAAGTAGCACTTGAAGAAGCTGCTAAAAATATTGAAAAGAAAGTTAATGATATTTTAATCAGAATACCAAATATTTCAATGGAAGATGTGCCACTTGGTAAAGACGAAAACCAAAATAAAGTTATAAAAAATCATGATAAATTAGGACGTGGATTAGTTTCAGGCGTTCAACCTCACTATGTTATAGCTAAAGACTTAGACATTATCGATCTTGAAAGAGCAGTAAAAATGTCAGGTTCAAGATTTGTAATTTACAAAGGTTTAGGTGCTAGATTAGCTAGAGCTATTATGAATTTTATGTTAGATTTACATACCAAAAATGGTTATGAAGAATATTCGGTACCTGTAATAGTTAAACCTGAAATTCTTTTTGGTACAGGTCAATTGCCTAAATTTAAAGAAGATCTTTTTGCTTTAGAAGATGCTCAAAGTTATTTAATTCCAACAGCTGAAGTCCCTTTGACAAACTACTATAATAATGAAATAATAGATTTATCAAAACCTATTAGAATGACAGCATTTACTGAATGTTTTAGATCAGAAGCAGGAAGTAGTGGAAAAGATACAAGAGGTATTATTAGAAACCACCAATTCAAAAAAGTTGAATTAGTAAAAATTGTTAGTGAAAAAGATTGA", "aa": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKD", "aa_hexdigest": "59c88f385cb38c1536d973d3b1ff70af", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 895310, "stop": 896494, "observations": { "mutation": [ 895480, 895615 ], "stop": [ 895480, 895615 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU2", "query_cov": 1.0, "subject_cov": 0.6926713947990544, "identity": 1.0, "score": 526.0, "evalue": 4.9e-186, "gene_start": 1, "gene_end": 293, "reference_start": 1, "reference_end": 293, "reference_sequence": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKDWEAEFNQMLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEVWLPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK" }, "paralog": false, "identity": 0.9949238578680203, "score": 727.0, "evalue": 2.03e-267, "description": "Internal stop codon at 895480, 895615. Nonsense mutation around 895480, 895615.", "psc": { "uniref90_id": "UniRef90_C4XFU2", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q6KIJ8", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU2", "EC:6.1.1.11", "UniRef:UniRef50_Q6KIJ8", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIJ8" ], "product": "Serine--tRNA ligase" } }, "id": "IGIGPGHMMC_1574", "locus": "GCA000148625_01570" }, { "type": "cds", "sequence": "contig_1", "start": 896756, "stop": 896905, "strand": "+", "frame": 2, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02914", "RefSeq:WP_013355004.1", "SO:0001217", "UniParc:UPI0001A8C0FF", "UniRef:UniRef100_C4XFU1", "UniRef:UniRef50_P05647", "UniRef:UniRef90_C4XFU1" ], "nt": "ATGAGTTTAAGAACATATCAACCAAACAAGACCCAACACATCAAGACTCATGGTTTTAGAGCAAGAATGGCAACAACAAATGGTAGAAAAGTTTTAGCTGCTAGAAGAGCTAAAGGTAGAGCAAGACTTACAGTTTCAGATGCTAAATAA", "aa": "MSLRTYQPNKTQHIKTHGFRARMATTNGRKVLAARRAKGRARLTVSDAK", "aa_hexdigest": "5c5a01ca21600f52eea0725329a3cdee", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C0FF", "ncbi_nrp_id": "WP_013355004.1", "uniref100_id": "UniRef100_C4XFU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0FF", "RefSeq:WP_013355004.1", "UniRef:UniRef100_C4XFU1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFU1", "uniref90_id": "UniRef90_C4XFU1" }, "psc": { "uniref90_id": "UniRef90_C4XFU1", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "IGIGPGHMMC_1575", "locus": "GCA000148625_01571" }, { "type": "cds", "sequence": "contig_1", "start": 897003, "stop": 897254, "strand": "+", "frame": 3, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "SO:0001217", "UniRef:UniRef50_A0A449BAF4", "UniRef:UniRef90_C4XFU0" ], "nt": "ATGATTATTTATTACATGCCTAACGATGATTTCAAAATTGGTATAACTGTACCGAAAAAGTTTTGCAATGCAGTTTTAAGAAATTACTATAAACGCCAATTAAAATCAATTATCCATTCTTTGAATTTGTATGGCGAAAAAATGCATTTTGTCTTAATTCCAAGAAAAGATTTTTTGAATTTAATATATGAAGATAAATTGACTATAACTAAAAAATTATTTGAAAAGATTAAAAATGGAAAACAAAAATAG", "aa": "MIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK", "aa_hexdigest": "8dcf24da0d4ca8bfcb2e97075703a42c", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnpA" ], "truncated": "5-prime", "pseudogene": { "start": 896910, "stop": 897254, "observations": { "mutation": [ 896952 ], "stop": [ 896952 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU0", "query_cov": 1.0, "subject_cov": 0.7280701754385965, "identity": 1.0, "score": 171.0, "evalue": 8.27e-54, "gene_start": 1, "gene_end": 83, "reference_start": 32, "reference_end": 114, "reference_sequence": "MVYKMKKIYRLQKSWEFDAVIKFQKQVLNKYMIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK" }, "paralog": false, "identity": 0.9824561403508771, "score": 228.0, "evalue": 1.5e-78, "description": "Internal stop codon at 896952. Nonsense mutation around 896952.", "psc": { "uniref90_id": "UniRef90_C4XFU0", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A449BAF4", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU0", "EC:3.1.26.5", "UniRef:UniRef50_A0A449BAF4", "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAF4" ], "product": "Ribonuclease P protein component" } }, "id": "IGIGPGHMMC_1576", "locus": "GCA000148625_01572" }, { "type": "cds", "sequence": "contig_1", "start": 897397, "stop": 897684, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCAAGACCTCAACATATACAGGTGCAGGTACTGAATATTACACATCATACGATAAAATTAGTCCTTATGTAACATCACTTGAACCAGCTAAAGCTGATAAAAAAATCGACAAAGATGACAAGGCTTTACAACCTGGTGAATTCAAGAGAATTGTTAAAAATGATTCTACAAACTACCATGCTAGCTACAAAAATCATGCCGAAATACTTAAAAAGTTAAGAGAGCAAACAGGCGGAGATGTTTATGGAAAATATAACTCATATTCATCTTCTATTCAATGA", "aa": "MVIKTSTYTGAGTEYYTSYDKISPYVTSLEPAKADKKIDKDDKALQPGEFKRIVKNDSTNYHASYKNHAEILKKLREQTGGDVYGKYNSYSSSIQ", "aa_hexdigest": "127ac08dfb145a7c55058a67d232ff9e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10740.811799999998, "isoelectric_point": 8.981364631652834 }, "id": "IGIGPGHMMC_1577", "locus": "GCA000148625_01573" }, { "type": "cds", "sequence": "contig_1", "start": 897778, "stop": 898434, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCGAATCTGCAACATATAAACCTACCTACACAACACCTTATAGTGAAATTAAATTTATTGACCCACAATTCAATTTAAATTCAATTTTATCTTTAGTTGATCAAAAAAATCCAGATCTTTATGAAGTTAAAAAAGATCGTTTAGAAAATAAAACATTCAAAACCCCTAAAAAAGCAACATTGTTTTCAATAGCTACACCAGACCAATTGAAAGCAAAAAATAAAATTGCTGGTAAGGAACTTAGTGAAATTGAGCTTTCAAAACAATACTATGCTCGTGATGTTTTAGAATTTTTATATAGAAAAACATTTATTGAACAAGTAAATGGTAAAGATAATTATTTTGCTAGAAGATTCAAACAAGAGTTTGGTACCGATAAATACAATGATGTATTAGGCAAAATAATTGATCCTACAAATACTAACAAATATAGTAAAAAACAAGTGGAACTTATTTATGAATATGTAAATATATTAAATGGATATCTTAAAACTACAGGTTTCACAAATATTTATGACGACACTCAATTAACAAAAGTTGAATTAGCTAAAGGAACTGAAAAAGTTAAAATAGCTAAAGAAAATGCTTATCTTTCTCCTGCTCCAAAACTTGTATACACTCATAGTGAACCATTCAAACCTTTTGTTAAATGA", "aa": "MSESATYKPTYTTPYSEIKFIDPQFNLNSILSLVDQKNPDLYEVKKDRLENKTFKTPKKATLFSIATPDQLKAKNKIAGKELSEIELSKQYYARDVLEFLYRKTFIEQVNGKDNYFARRFKQEFGTDKYNDVLGKIIDPTNTNKYSKKQVELIYEYVNILNGYLKTTGFTNIYDDTQLTKVELAKGTEKVKIAKENAYLSPAPKLVYTHSEPFKPFVK", "aa_hexdigest": "ab30415c63ae82051cba3bfaaa97e8e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25326.61180000002, "isoelectric_point": 9.24284839630127 }, "id": "IGIGPGHMMC_1578", "locus": "GCA000148625_01574" }, { "type": "cds", "sequence": "contig_1", "start": 898699, "stop": 898812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAAGACATCAACAAGAAATTGCGGCTTTATATAAAAAGAATAATATAAATCCTCTTGATGCTTTCTTGAATATAATTATTTCCTTCCCTATCTTCATTGCTATGTGA", "aa": "MKIRHQQEIAALYKKNNINPLDAFLNIIISFPIFIAM", "aa_hexdigest": "b7c63e99f55e16ffee088a48f7a7973d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4330.1669, "isoelectric_point": 9.522834587097169 }, "id": "IGIGPGHMMC_1579", "locus": "GCA000148625_01575" }, { "type": "cds", "sequence": "contig_1", "start": 899344, "stop": 899682, "strand": "+", "frame": 1, "gene": null, "product": "Nucleoside 2-deoxyribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8", "UniRef:UniRef90_C4XFT8" ], "nt": "ATGAAAAAAATACATGCTTATGTAGCTGGTCCATTGTTTACTAGAGCAGAAATTGACTTAAGATACGCAATCGAAGAAACAATGAAAAAAGCACTAAAATCAAAAGAATTAAAAGGCAAAATTGACTTTGACATTTTCAATCCGATTCATCTAAATGAAGAATTAGAACAAAATGGAAAATTAACACCACAAGAAATTTTCAAAAATGACTTAGCAGCAATTCAAAAAAGTAAACTTACAATTCTTGACATTGACAACAAAGATGATGGCACCATGGCAGAATTTGGTTACTTCTTAGCAATGAAAGAAAGAGATCCAGAAGTTAAAATATGTGTTTGA", "aa": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICV", "aa_hexdigest": "5019ddcd36705ab0d43f0b32dc87cfb5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 899344, "stop": 899817, "observations": { "mutation": [ 899680, 899794 ], "stop": [ 899680, 899794 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT8", "query_cov": 1.0, "subject_cov": 0.7133757961783439, "identity": 1.0, "score": 223.0, "evalue": 4.14e-73, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICVWMSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYDWLIKAYK" }, "paralog": false, "identity": 0.9872611464968153, "score": 308.0, "evalue": 3.24e-109, "description": "Internal stop codon at 899680, 899794. Nonsense mutation around 899680, 899794.", "psc": { "uniref90_id": "UniRef90_C4XFT8", "product": "Nucleoside 2-deoxyribosyltransferase", "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT8", "UniRef:UniRef50_C4XFT8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8" ], "product": "Nucleoside 2-deoxyribosyltransferase" } }, "id": "IGIGPGHMMC_1580", "locus": "GCA000148625_01576" }, { "type": "cds", "sequence": "contig_1", "start": 899683, "stop": 899796, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATTTTAGAGATGTAGCAGATCGCGACATTAGATTAAACAGATTTATCAACGGAATGATTTATGTATCAGATGGTTGCGTAAAAAATCAACAAGAACTATATGATTGA", "aa": "MSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYD", "aa_hexdigest": "bd75a301a6f1d5c4e7105b81e844b134", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4397.880000000001, "isoelectric_point": 4.658887672424316 }, "id": "IGIGPGHMMC_1581", "locus": "GCA000148625_01577" }, { "type": "cds", "sequence": "contig_1", "start": 899895, "stop": 900065, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAATAATTTAATCGAAAATAAATTAATTGCTTCAATGCGCGGCATAGCATTGGATTCAATCAACAAAGCAAAAGGTGGCCACATTGGTATGGCTATTGGAGCTGCAAATATTACTTATTCATTATTAGGAAAGGAATTAAGATTCTCAAAAGAAGATCCGAAATGA", "aa": "MKNNLIENKLIASMRGIALDSINKAKGGHIGMAIGAANITYSLLGKELRFSKEDPK", "aa_hexdigest": "6d4df5968824f334d0f4f49936664834", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 49, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 2.2152661234010214e-08, "score": 29.43653106689453, "start": 7, "stop": 55 } ], "seq_stats": { "molecular_weight": 6043.0491999999995, "isoelectric_point": 9.775808525085449 }, "id": "IGIGPGHMMC_1582", "locus": "GCA000148625_01578" }, { "type": "cds", "sequence": "contig_1", "start": 900108, "stop": 900818, "strand": "+", "frame": 3, "gene": null, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "nt": "ATGTCATATTATTCAATTATGCATTTCTTAGGTTTATTAGCAAAAGAAGACATGCAAAATCATAAAAAAATGGACTCAAAAACACCTTCTCATCCTGAGTTAGAATATACACCTTTTGTGGATGCTTCAACAGGTCCTTTAGGTCAAGGAATAGCTATGGCTACTGGTATGGCTCTTAGTCTAAAAGTACTACAAAACAGGTTTAATCGTAAAAATTACAATATTATAGACCACTACATTTATGCGCTTCATGGAGACGGTTGTATTCAAGAAGGTGTGGCTCATGAAGCTATTCAATTTGCAGGTACAAATCAATTAGATAGACTTATTTTAATTCATGATTTTAATAATGCTCAAATTGATACAAAAACCGAGGTTGTTAATAATATTAACTTCATTAAATATTTTGAAGCTTGTCATTTTGCAACCTTTGTAGTTAAAGAAGATAAACCTGAATTAATTCTTGAAGCAATTCAAAAAGCTAAAGCTTCTAAGAAACCTTCATACATTCAAGTTCACACAGCAATAGCTAAAAATACACCTTTTGAAAACTCGCCAAAAGGTCACCATGGAATGTTAAAAGAAGAAGATACTATTGAATTTAAAAAGAAATGTGGTTTAACTTCATTTGTTCCTTTTGAATATGACAAAGAAGTTTATGACTATGGAGAAGAATTATTAAAAGAAAAAGACAAACATTATAAAGAATGA", "aa": "MSYYSIMHFLGLLAKEDMQNHKKMDSKTPSHPELEYTPFVDASTGPLGQGIAMATGMALSLKVLQNRFNRKNYNIIDHYIYALHGDGCIQEGVAHEAIQFAGTNQLDRLILIHDFNNAQIDTKTEVVNNINFIKYFEACHFATFVVKEDKPELILEAIQKAKASKKPSYIQVHTAIAKNTPFENSPKGHHGMLKEEDTIEFKKKCGLTSFVPFEYDKEVYDYGEELLKEKDKHYKE", "aa_hexdigest": "ecb7c1c3978c878450d8790505a99ccc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF16CA3", "query_cov": 0.809322033898305, "subject_cov": 0.880184331797235, "identity": 0.513, "score": 204.0, "evalue": 6.83e-63, "valid": false, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI002FF16CA3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF16CA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1583", "locus": "GCA000148625_01579" }, { "type": "cds", "sequence": "contig_1", "start": 900966, "stop": 901700, "strand": "+", "frame": 3, "gene": null, "product": "transketolase-like TK C-terminal-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "nt": "ATGCAAAAAATAGATCAAAATTACAAAAACGTTTTAGGTGGTTCAGCTGACTTAGCCTCAGCTACAAAAGTTAACTTTAGCAAATCTTATGCTGAAGGTGGCAAAAACATTCACTATGGAATTAGAGAATTTGCTATGACTGCTATAAATAATGGAATCTATTTACACTCACATTTAAGAACAATAGATTCAACATTCTTAGCTTTTAGCGATTATGCTAAAGGTGCTCTAAGACTAGGTTCAATTATGAAAATACCTTCAGTTCATGTTTACACTCATGATAGTTACCAAGTTGGGGGTGACGGACCAACTCATCAACCAGTAGAACAATTAGCAATGCTTAGAAGTACTCCAAATTTTAAAGTTATTAGACCTATGGATGAGAGCGAAGTCAAAGCAGCATTCAATTATGCATTAAATCAAAATGAAAATCAAGTAGCTATTATTGGTTGTCGTCAAAATGTTAAATCTTACAATTTACATCCTGAAGGTGAATTGCAACCAGCTTACATTATTCGTCCTGCAAAAAACCATGACATTAGTATTTTGGCTTCTGGTAGTGAAGTTGACTTAGCAGAAAAGGTTGCTAATAAATTAGTTGGAGCAGGCATTAAAGCTCAAGTTATTTCCGTTCCTTTATTACAAGATTTAGTTAATGATGAAAAATTAATCAAAAGCTTAGGCATTGACAAAAAACCAATATATGCTATTGAAGCTTCAACAGATCCATTGTGA", "aa": "MQKIDQNYKNVLGGSADLASATKVNFSKSYAEGGKNIHYGIREFAMTAINNGIYLHSHLRTIDSTFLAFSDYAKGALRLGSIMKIPSVHVYTHDSYQVGGDGPTHQPVEQLAMLRSTPNFKVIRPMDESEVKAAFNYALNQNENQVAIIGCRQNVKSYNLHPEGELQPAYIIRPAKNHDISILASGSEVDLAEKVANKLVGAGIKAQVISVPLLQDLVNDEKLIKSLGIDKKPIYAIEASTDPL", "aa_hexdigest": "5fe999d8aafce3b0e00b39e66143a0c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080BCD6", "query_cov": 0.9590163934426229, "subject_cov": 0.8447653429602888, "identity": 0.581, "score": 247.0, "evalue": 9.65e-79, "valid": false, "product": "transketolase-like TK C-terminal-containing protein", "uniref50_id": "UniRef50_UPI0026B1FBDD" }, "pscc": { "uniref50_id": "UniRef50_UPI0026B1FBDD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "product": "transketolase-like TK C-terminal-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1584", "locus": "GCA000148625_01580" }, { "type": "cds", "sequence": "contig_1", "start": 903178, "stop": 903618, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13240.11", "PFAM:PF17032.10", "PFAM:PF13248.11" ], "nt": "ATGGAAGTTGTTAACTCAAGCATTGAAGATATTAGTATACCTAAAGAGCAACAAGATGAATTGAACAAAATTCTTAAAAGAAAAGCCGAAGTTGATATCATGGGAGACGACTATGCTAAAGTTAGAGGCTATGACGTTTACCAAGATGCAGCAAACAATTCGGGTTCAGTAGGAACTTTTTTTGGAGCAGGCATTGGTTTAGGACTAAACGAAGGTGCTTCAAATTTATTAAGTGATAGCAAAATCAATAAATCAGCAACCCCAACAGCAGCAGCTGCATTATTAATCACTTGCGGTACATGTGGAAGCAAAGTATCTAAAGATTCTAAATTTTGTTTTGAATGTGGTTCAAAAATCGCAACAAACAAATGTTCATCATGTAATATCGACTTATCGCCAAATGCAAAATTCTGCGTAAATTGCGGAAAGAAGGTGAAATAA", "aa": "MEVVNSSIEDISIPKEQQDELNKILKRKAEVDIMGDDYAKVRGYDVYQDAANNSGSVGTFFGAGIGLGLNEGASNLLSDSKINKSATPTAAAALLITCGTCGSKVSKDSKFCFECGSKIATNKCSSCNIDLSPNAKFCVNCGKKVK", "aa_hexdigest": "98759b982d361b41e92adc86842ff972", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13240.11", "name": "Zn_Ribbon_1", "length": 23, "aa_cov": 0.15753424657534246, "hmm_cov": 1.0, "evalue": 2.4010893443144753e-12, "score": 42.3330192565918, "start": 123, "stop": 145 }, { "id": "PF17032.10", "name": "Zn_ribbon_15", "length": 22, "aa_cov": 0.1506849315068493, "hmm_cov": 1.0, "evalue": 2.42304798001768e-08, "score": 30.724994659423828, "start": 95, "stop": 116 }, { "id": "PF13248.11", "name": "Zn_ribbon_3", "length": 24, "aa_cov": 0.1643835616438356, "hmm_cov": 1.0, "evalue": 2.966707267695801e-09, "score": 32.15855026245117, "start": 96, "stop": 119 } ], "seq_stats": { "molecular_weight": 15387.314299999987, "isoelectric_point": 7.450250434875489 }, "id": "IGIGPGHMMC_1585", "locus": "GCA000148625_01581" }, { "type": "cds", "sequence": "contig_1", "start": 904067, "stop": 904165, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACAGATCAAAAATTTAAATCAAGAGAAAGATCACTTAGAGAAGAATACATCACAGGACAAAAACCTAAAGATGAAGAAGGGTTGTACTAA", "aa": "MSKTDQKFKSRERSLREEYITGQKPKDEEGLY", "aa_hexdigest": "a36ef443794295c5669fa644944a48b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3878.2836000000007, "isoelectric_point": 8.065011024475098 }, "id": "IGIGPGHMMC_1586", "locus": "GCA000148625_01582" }, { "type": "cds", "sequence": "contig_1", "start": 904168, "stop": 904752, "strand": "+", "frame": 1, "gene": null, "product": "Zinc ribbon domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT3", "UniRef:UniRef90_C4XFT3" ], "nt": "ATGGAATGCAAATCATGTGGAGCTAATGTTAGAATCACAGATAAATTTTGTTCTTTTTGCCGATCATTAATCGAAGCACCTAAATCAAGCGTTGAAACATCTCAACACACAACTGCAACTACAAGAGTAGTAACAGAATCAAAAGCAATTAATTATGATGTATTGAACTTATTAGTTATAGAAGATAAATATGACGAAATTGATTTTGATTTATCTTCTAACACAGCTGCAAACTCAATGAAAACATATGTAAACAAAGGAGGAAGCATTTCAGAACTTGAGAAAAGTTATGTTTTAGCAAATAAAATCGATTACTATGAATCACTTGACAAAGATGAAATCTTAAAAACTTACTCAGAAAAAATTAAGAAATATTCCGGTAAAGATATTTGTTGTTTAAAAAGAAGTGAATTACAGGCCTTAGAAAGAAAAATGAGTTTATATGAATTCAATGATTATCTTAAAGTATTAAAAGATAGAGTATTTAATAAAGAAGGAATTATGCTAAGTGACAGCGACCTACTCGACAAAATAAACTCAGATAGAAACATTGAAGTTTTTGCTATTTGTTCTTATGACTTCTGA", "aa": "MECKSCGANVRITDKFCSFCRSLIEAPKSSVETSQHTTATTRVVTESKAINYDVLNLLVIEDKYDEIDFDLSSNTAANSMKTYVNKGGSISELEKSYVLANKIDYYESLDKDEILKTYSEKIKKYSGKDICCLKRSELQALERKMSLYEFNDYLKVLKDRVFNKEGIMLSDSDLLDKINSDRNIEVFAICSYDF", "aa_hexdigest": "e1ba4f6eb62a89dc85fabb2ea897b1f5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 904165, "stop": 904965, "observations": { "mutation": [ 904750, 904852, 904897 ], "stop": [ 904750, 904852, 904897 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT3", "query_cov": 1.0, "subject_cov": 0.7293233082706767, "identity": 1.0, "score": 373.0, "evalue": 1.91e-129, "gene_start": 1, "gene_end": 194, "reference_start": 2, "reference_end": 195, "reference_sequence": "MMECKSCGANVRITDKFCSFCRSLIEAPKSSVETSQHTTATTRVVTESKAINYDVLNLLVIEDKYDEIDFDLSSNTAANSMKTYVNKGGSISELEKSYVLANKIDYYESLDKDEILKTYSEKIKKYSGKDICCLKRSELQALERKMSLYEFNDYLKVLKDRVFNKEGIMLSDSDLLDKINSDRNIEVFAICSYDFWDKKTVELDRQNKVEYEQRIKRINNKKKTAKVIIWTIVGIFISIFIICFLVALGVTIRSSVSIASTSSSYY" }, "paralog": false, "identity": 0.9473684210526315, "score": 493.0, "evalue": 3.31e-179, "description": "Internal stop codon at 904750, 904852, 904897. Nonsense mutation around 904750, 904852, 904897.", "psc": { "uniref90_id": "UniRef90_C4XFT3", "product": "Zinc ribbon domain-containing protein", "uniref50_id": "UniRef50_C4XFT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT3", "UniRef:UniRef50_C4XFT3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT3" ], "product": "Zinc ribbon domain-containing protein" } }, "id": "IGIGPGHMMC_1587", "locus": "GCA000148625_01583" }, { "type": "cds", "sequence": "contig_1", "start": 905194, "stop": 905298, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAAATCAATTTAGTATCAAGATTTATTGATAAAAAAATCAAACTTGAACACTTGTCTTTTGAAAATGAAGTAGAAAAACATGATGCATTAAGACAATGA", "aa": "MNEINLVSRFIDKKIKLEHLSFENEVEKHDALRQ", "aa_hexdigest": "1b6b5d68af35965b1a0d7484f9e95523", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.677600000001, "isoelectric_point": 6.028479957580566 }, "id": "IGIGPGHMMC_1588", "locus": "GCA000148625_01584" }, { "type": "cds", "sequence": "contig_1", "start": 905485, "stop": 905817, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGCTCTAAGTGGCAAAACAGTTTTAAATGAAAAATTTAATGAAATTGTTCAACAAAAAATTGAATTAGAAAAGAAATATGGAGCATTTATTTACTTTGATGTAATTGTTAAAGAAGAAACTTTAACCTTAGATAATTGTGACTACCAAGTTATCATGGAAACATTCAAACCAAATGAAATTGCTAAAATTAATTTAGTTCCTAAAGACTATATTCCAGCTTTTGATCCTTTACCAATTAATAAATTAGAAGAATATGCAATGGAATATAATCCAGGCGGTTCAAAAAATTTAGCAAAAGAAGAATTAAATGAAATTAAAAGATGA", "aa": "MLIALSGKTVLNEKFNEIVQQKIELEKKYGAFIYFDVIVKEETLTLDNCDYQVIMETFKPNEIAKINLVPKDYIPAFDPLPINKLEEYAMEYNPGGSKNLAKEELNEIKR", "aa_hexdigest": "5929d454884129dd65bcfe5a2b6dc57d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12746.611199999985, "isoelectric_point": 4.814740180969238 }, "id": "IGIGPGHMMC_1589", "locus": "GCA000148625_01585" }, { "type": "cds", "sequence": "contig_1", "start": 906013, "stop": 906609, "strand": "-", "frame": 2, "gene": null, "product": "ribonuclease HII", "db_xrefs": [ "EC:3.1.26.4", "GO:0003723", "GO:0004523", "KEGG:K03470", "RefSeq:WP_013355014.1", "SO:0001217", "UniParc:UPI0001E33051", "UniRef:UniRef100_C4XFT1", "UniRef:UniRef50_I5D6C7", "UniRef:UniRef90_C4XFT1" ], "nt": "ATGTTGAATTATGAAAAAGAAAATTATCCTGACACTAAATTAATTGTAGGCTTAGATGAAGCAGGAAGAGGTTGTTGTGCAGGGCCTTTAGTAGTTGGTTGTGTTATTATGCCTCAAAATTTTAACCATCCTTTAATAAAGGATTCTAAACAATTGAGTCAAAAACAACGTGAGGAAGCATTAGAAATTATTCTTGAAAATGCTATTGAAACAGTAACAACTATTATTAATGCTCAAGAAGTAGATCAACTAAATCCAAAGCAAGCTTCAAGAATTGGCATGCAAAGGTGTATTAATAAACTTAATACAAAACCTGAATTAGTAATTACAGATTTTGAAAAAATTAATGACATCAATATACCTCAAATTAATTTAGTAAAAGGTGACCAATTAAGTATTAATGTGGCAGCAGCTTCAATTTTAGCTAAAGTAACTAGGGACTTAATTATGGTTGGAATTGACAAAAAATTTCCTATGTATAATTTCAAGAAAAATAAGGGCTATTGTACAAAAGAACATGAAGCAGCAATGAGTCAATATGGCATCTGTGAATTTCATAGAAAGTCATATAAAAATGTTAAAAAATATTTGAATTAA", "aa": "MLNYEKENYPDTKLIVGLDEAGRGCCAGPLVVGCVIMPQNFNHPLIKDSKQLSQKQREEALEIILENAIETVTTIINAQEVDQLNPKQASRIGMQRCINKLNTKPELVITDFEKINDINIPQINLVKGDQLSINVAAASILAKVTRDLIMVGIDKKFPMYNFKKNKGYCTKEHEAAMSQYGICEFHRKSYKNVKKYLN", "aa_hexdigest": "4b51a8bd10e0b19c82873a8af19a91ac", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33051", "ncbi_nrp_id": "WP_013355014.1", "uniref100_id": "UniRef100_C4XFT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33051", "RefSeq:WP_013355014.1", "UniRef:UniRef100_C4XFT1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFT1", "uniref90_id": "UniRef90_C4XFT1" }, "psc": { "uniref90_id": "UniRef90_C4XFT1", "product": "ribonuclease HII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_I5D6C7", "kegg_orthology_id": "K03470", "go_ids": [ "GO:0003723", "GO:0004523" ] }, "pscc": { "uniref50_id": "UniRef50_I5D6C7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D6C7" ], "product": "Ribonuclease HII" }, "genes": [], "id": "IGIGPGHMMC_1590", "locus": "GCA000148625_01586" }, { "type": "cds", "sequence": "contig_1", "start": 906663, "stop": 907223, "strand": "-", "frame": 3, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0", "UniRef:UniRef90_C4XFT0" ], "nt": "TTGTTCTCAATTATTAGAAACATTAGATCACTAGCTACTGCTTACAATCCAATTATTTTATCAATAACAATAGTTACTTCAGTTATCACAGTTGTTTTAATTGTTTTCCAATTCTTGTTTGTTAGTCAATTAAGATACTTCAAAGGTGTATTTATTAATGCAAGAAGATTAGAAGAAATCAAACAACTTCAAAAAAAGATGAAAGAATCAGGTATGATGGATGAAAGCAATTTAGCAAACAATCTTTTTGGAATCATGTTAGATGATGTTGATGATGTAAGTGCTAATCAAGATAAAAGCAAATTCAATAAAGAAGAAGATATTGAAGAAGCTGAAATAGTTGATAACAAAGCACATCTTAAAAATAATGAAAAAGAAAATGAAGAAAAAATCAAAAAATTATTAGCATTGCCTAATGCAAAACTTTACAACATAGCAGAAACCCTATACATTAGTGGTTACAAAAAAATAGACAAAGAAGAATTATGCCGCTTAATTGTTCAAATTGTTGATACTCAAAAGGCTAAAGAAGACGAAGAAGCAAACAAAGACAAAAAATAA", "aa": "MFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKIDKEELCRLIVQIVDTQKAKEDEEANKDKK", "aa_hexdigest": "e10c357209c90853ce9077839681cc4d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 906663, "stop": 907523, "observations": { "mutation": [ 907235, 907262 ], "stop": [ 907235, 907262 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT0", "query_cov": 1.0, "subject_cov": 0.577639751552795, "identity": 0.9890000000000001, "score": 328.0, "evalue": 6.02e-111, "gene_start": 1, "gene_end": 186, "reference_start": 137, "reference_end": 322, "reference_sequence": "MDIGEKMNLFDKKYLQIKSTNKLWALEKERFRPVVILSLLTFFLALACALTSTILLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHTWPSMVLIISWFINLFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKMDKEELCRLIVQIVDTQKAKEDEEANKDKK" }, "paralog": false, "identity": 0.9895104895104895, "score": 538.0, "evalue": 4.19e-196, "description": "Internal stop codon at 907235, 907262. Nonsense mutation around 907235, 907262.", "psc": { "uniref90_id": "UniRef90_C4XFT0", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT0", "UniRef:UniRef50_C4XFT0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "IGIGPGHMMC_1591", "locus": "GCA000148625_01587" }, { "type": "cds", "sequence": "contig_1", "start": 907260, "stop": 907469, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCGCTGCTTTTAAAGATAATTTAGTTGACTATATTGTAAAAATTAATGGTGGTACAACTCCAAGTTTTAAACCTGAAGATCAAATAATGAGCATGATTATTACAAATGGTTTAGTTACAGTTGGTATGTTAGTTAGCATTATTGTATTTTTAGTTTTTTATGCTCGAACATTTAAAGAAAAATCATTAGCCAACATGCATACATGA", "aa": "MLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHT", "aa_hexdigest": "62d54fabd05170d1bf1e5153377c760e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7685.072599999998, "isoelectric_point": 8.085834312438966 }, "id": "IGIGPGHMMC_1592", "locus": "GCA000148625_01588" }, { "type": "cds", "sequence": "contig_1", "start": 907640, "stop": 908563, "strand": "-", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_013355016.1", "SO:0001217", "UniParc:UPI0001E33053", "UniRef:UniRef100_UPI0001E33053", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_C4XFS9" ], "nt": "ATGTTAAAAATTAGTGTTCAATATAAAGAGAGAATCGATAAGTATATCGGTCAAAATAGTGAATTAAGTCGAAATGATATTAAAGAGTTAATTGAGCAAGGTGCAGTTTATATTAATGACAACGTTCAAGTACATAAATGTAATTACATCGTTCGCGAAGGACAAGAAATTACAATTGTTAAATTACTTGACAAAGAAATTAAAATTGAACCAGTTAATATTCCTCTTGACATTAAATATGAAGATGATGATTTAGTAATAATTAATAAACCAAGTAATCTAGTTGTCCACCCTTCACCGGGGCATTACAATGACACACTAGTTAATGGACTTTTATATCATTTTAAAAACAATTTATCAAATGAAAATGGGTTACTTCGACCAGGTATAGTACACCGTATTGATAAAGACACAAGTGGCTTATTAATAATTGCTAAAAATAACAAGATGCATAACTCATTAACTGACATGCTCAAAAGACATGAAATTGAAAGAAGTTATTTAGCTATCGTTAGAGGCTTAATTGAAAATCCACTTACTAAAATTAATTTACCAATTGCTAGAAATACTGTTGATCGTAAAATGATGTCAGTTCATAAAGAAGGTAAAGAAGCTATAACCTTTGTTCATTTGCTTAAGCACTTTTATTTAGATAAAGAACCAATGTCTTTAGTTAGATGCGAATTAAAAACAGGTCGAACTCATCAAATTAGAGTCCACTTGTCTTACATCAAACATCCAGTTTACGGTGATCCTACTTATGGCAAAAGTGTTGATGAATTTAATCAAAGATTACATGCTTATAAATTAAAATTTATTCATCCACTTACTAAAAAAGAAATTTGTGTTTATGCACCGGTGCCTAAAGAATTTGATGTAGCCGATTATGATTTTAATGAACTATTAAAAAATAATGTTTTATAA", "aa": "MLKISVQYKERIDKYIGQNSELSRNDIKELIEQGAVYINDNVQVHKCNYIVREGQEITIVKLLDKEIKIEPVNIPLDIKYEDDDLVIINKPSNLVVHPSPGHYNDTLVNGLLYHFKNNLSNENGLLRPGIVHRIDKDTSGLLIIAKNNKMHNSLTDMLKRHEIERSYLAIVRGLIENPLTKINLPIARNTVDRKMMSVHKEGKEAITFVHLLKHFYLDKEPMSLVRCELKTGRTHQIRVHLSYIKHPVYGDPTYGKSVDEFNQRLHAYKLKFIHPLTKKEICVYAPVPKEFDVADYDFNELLKNNVL", "aa_hexdigest": "961f68bb50eea654c0c9044a5bbb9d7c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33053", "ncbi_nrp_id": "WP_013355016.1", "uniref100_id": "UniRef100_UPI0001E33053", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33053", "RefSeq:WP_013355016.1", "UniRef:UniRef100_UPI0001E33053" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33053", "uniref90_id": "UniRef90_C4XFS9" }, "psc": { "uniref90_id": "UniRef90_C4XFS9", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [], "id": "IGIGPGHMMC_1593", "locus": "GCA000148625_01589" }, { "type": "cds", "sequence": "contig_1", "start": 908745, "stop": 909008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAACTTATTTAGTCTTTGTTATTCCACCAATTTTAATGATGAAATTAGCAATGAAATATTCAAAAAAATTTGAAAATAATAAATATTTAATAAAATTAATTAACATAATGAATCCTGTTGTAACAGGTATTATTGTTGCCTTGGGTTTTCAACTTTTTATAGCTTCAGTAGCACCTCAAGTTTTCTTTAATAAATCATTTACTAACTATGTAGGAATAAATAATGATACACAAAAAGCACTGTTTTATAGGGGTTGA", "aa": "MFLTYLVFVIPPILMMKLAMKYSKKFENNKYLIKLINIMNPVVTGIIVALGFQLFIASVAPQVFFNKSFTNYVGINNDTQKALFYRG", "aa_hexdigest": "ff114127c916604c6f5209d78b9669e8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10004.98719999999, "isoelectric_point": 9.932079887390138 }, "id": "IGIGPGHMMC_1594", "locus": "GCA000148625_01590" }, { "type": "cds", "sequence": "contig_1", "start": 909269, "stop": 909475, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGTAATAATTATGGTTCTAATTATTTGTATTTTTGCAACTGTTGAAGCAATCAAACACAAAAAAGAAATCAAAAAACTTAAAGAAAATATTGAAAATAAAGTTACTAATTTAGACAATAACATAGAAGAAAAAATAGAACAAAACTTAGAATTAGAGACCAAAGAAAATGATTTGAATAGTGACAAGAAAGGAGGTCAATAA", "aa": "MPVIIMVLIICIFATVEAIKHKKEIKKLKENIENKVTNLDNNIEEKIEQNLELETKENDLNSDKKGGQ", "aa_hexdigest": "e56a278954b69d1ea5da6add22e1ddca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7834.0481999999965, "isoelectric_point": 5.3715917587280275 }, "id": "IGIGPGHMMC_1595", "locus": "GCA000148625_01591" }, { "type": "cds", "sequence": "contig_1", "start": 909533, "stop": 909799, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCAGGTCCTAGCGTCATTGAAGCAATTAGTTATATTACTATTAAACTTTTAGGTTTCAAAAAGGGCTTGTTTTTGGCAACTTTTGCAACCTTACCTCATGTTTTAGTTTTCTTTTTAATCTTTTATTTTGTGCAACATATTCCTCAAAAATATTTGTTTGTTATTGAACTTGGTGTTATTGTTGCAATTATTGGATCATTGATTGGTTTTGCTTGGGAATACTTCAAAAAATCAAGAGGCAAAACTAAAATTAGTTTGTGA", "aa": "MLPGPSVIEAISYITIKLLGFKKGLFLATFATLPHVLVFFLIFYFVQHIPQKYLFVIELGVIVAIIGSLIGFAWEYFKKSRGKTKISL", "aa_hexdigest": "1faedfa2cefe365f13dafcceb95d05f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9952.00599999999, "isoelectric_point": 9.853041648864746 }, "id": "IGIGPGHMMC_1596", "locus": "GCA000148625_01592" }, { "type": "cds", "sequence": "contig_1", "start": 910158, "stop": 910397, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAAAAAATGTTTATCAATTGCAAAGAGAAAAATTATTAAAAAATGCTTTTATTGACAAAAAATTGAGAAAAGAAAATATCGAAAAACTTTACTCAATAATTGTGCAAAATGAAAGTAAGCTTAAAGAAGCTTTATCTTTGGACCTTAATAAATCATCACTAGAATCATATTTTAGTGAGATAGGTTTAGTCTTAAAAGAAATCAAATTTAATATTAAAAATTTAAATAAATGA", "aa": "MNAKNVYQLQREKLLKNAFIDKKLRKENIEKLYSIIVQNESKLKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNK", "aa_hexdigest": "a74f68ee47e5a3bf1b4c7ea924e9eb49", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9301.804799999993, "isoelectric_point": 9.619085884094236 }, "id": "IGIGPGHMMC_1597", "locus": "GCA000148625_01593" }, { "type": "cds", "sequence": "contig_1", "start": 910521, "stop": 910880, "strand": "+", "frame": 3, "gene": null, "product": "aldehyde dehydrogenase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E33056", "UniRef:UniRef90_UPI0001E33056" ], "nt": "ATGCCATTAGTTTCAGCTCTTGCAGCAGGCAATAGAGTAATCATTAAACCAAGTGAATTTTCAGTCCACACTTCACAATTATTACAAGAATTAATCAACAATAATTTTGATCCTCAATATGCTTTTGTAGCAGACAGTTCTTTAGAAGTTGCTCAAGAATTGTTGGACTACAAATTTGATTTAATTCATTTCACAGGTAGCACAACAGTTGGAAAAATTATTGCTAAAAAAGCATCTGAAACTTTGACACCAATTACTTTAGAATTAGGTGGGAAGTGCCCTTGCGTTGTTGACAAAAGTCAAGACATAATTTATTCTGCTCAAAAAATTATGGCTGGTAAATTGCTTAATGCAGGTTAA", "aa": "MPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCPCVVDKSQDIIYSAQKIMAGKLLNAG", "aa_hexdigest": "e52e5ae51bc80f47b1b3ff3d8e7789b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 910221, "stop": 910880, "observations": { "mutation": [ 910395, 910494 ], "stop": [ 910395, 910494 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E33056", "query_cov": 1.0, "subject_cov": 0.49583333333333335, "identity": 1.0, "score": 234.0, "evalue": 3.25e-76, "gene_start": 1, "gene_end": 119, "reference_start": 122, "reference_end": 240, "reference_sequence": "MNAKNVYQLQREKLLKNAFIDKKLRKENIEKLYSIIVQNESKLKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNKWTKPFHVSTPSHLALGKSEIQYEPLGQVLIISPWNYPFYLSLMPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCPCVVDKSQDIIYSAQKIMAGKLLNAG" }, "paralog": false, "identity": 0.9908675799086758, "score": 418.0, "evalue": 2.47e-151, "description": "Internal stop codon at 910395, 910494. Nonsense mutation around 910395, 910494.", "psc": { "uniref90_id": "UniRef90_UPI0001E33056", "product": "aldehyde dehydrogenase family protein", "uniref50_id": "UniRef50_UPI0001E33056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E33056", "UniRef:UniRef50_UPI0001E33056" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E33056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E33056" ], "product": "aldehyde dehydrogenase family protein" } }, "id": "IGIGPGHMMC_1598", "locus": "GCA000148625_01594" }, { "type": "cds", "sequence": "contig_1", "start": 910968, "stop": 911114, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAATGAAGAATATATTAAAGGTAATTATCCAAAGATAATTAATCAAAAACATTTTGAAAGATTAAAAAAATATTTACCTAAAGATCATAAAATTAATGAAAAAACATTACAAATAATGCCTTATATTTTTGAAACAAGCTGA", "aa": "MQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETS", "aa_hexdigest": "169495a1d7138bc6d76f3489c26f6581", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5940.8462, "isoelectric_point": 9.302094841003417 }, "id": "IGIGPGHMMC_1599", "locus": "GCA000148625_01595" }, { "type": "cds", "sequence": "contig_1", "start": 911127, "stop": 911486, "strand": "+", "frame": 3, "gene": null, "product": "aldehyde dehydrogenase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1", "UniRef:UniRef90_UPI0002E0F0E1" ], "nt": "ATGCAAGAAGAAATTTTTGGGCCGTTGTTGCCTATTATTCCTTATGATAATTTAGATGATTTATTAGTCAAATTTAAATCACTTCCTAAACCTTTAGCTGCTTATTTATTTACTAAAGATAAGAAAATAATAAAGCTATTTAAAGAACAAGTTACTAGTGGAGCTTTAGTAATTAATGATACCATTATGCATATAGCAAATAGTAATTTACCTTTTGGTGGTGTTGGTGATTCTGGCTATGGCAGAAGTCATGGTTTTGAAGGTTTTAAAGCTATGTCAAATCCAAAAGCTATTTACCAAAGAAAAGGTTTTAATTTTAAATTAAATGAGCATCCTTACAGCAAGAAAAAAGAATTCTGA", "aa": "MQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEF", "aa_hexdigest": "1ba18871fb632d394b157164d06658a4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 910968, "stop": 911507, "observations": { "mutation": [ 911112, 911484 ], "stop": [ 911112, 911484 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "query_cov": 1.0, "subject_cov": 0.664804469273743, "identity": 1.0, "score": 242.0, "evalue": 2.76e-80, "gene_start": 1, "gene_end": 119, "reference_start": 54, "reference_end": 172, "reference_sequence": "MQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETSWNDLIMQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEFWIKKIIK" }, "paralog": false, "identity": 0.9832402234636871, "score": 358.0, "evalue": 1.54e-128, "description": "Internal stop codon at 911112, 911484. Nonsense mutation around 911112, 911484.", "psc": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "product": "aldehyde dehydrogenase family protein", "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002E0F0E1", "UniRef:UniRef50_UPI0002E0F0E1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1" ], "product": "aldehyde dehydrogenase family protein" } }, "id": "IGIGPGHMMC_1600", "locus": "GCA000148625_01596" }, { "type": "cds", "sequence": "contig_1", "start": 911601, "stop": 911879, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5", "UniRef:UniRef90_C4XFS5" ], "nt": "ATGTTGCCTTATATTATTGTTGGCTACAGTCATGAAAAATTAAAATATTTTTCGCATGTCAATATATCGTTAGGAGTAATCTTAATTATCTTAACTATTTCGCAAGTAGCAGTTTCAACTAAAGCAAATTTCCTTATTTATTTCTTAATAAGCTTTGAAATAATTAACGTTATTTGCATAACTATTAGTTTGATTTTTGGCATTATTTTCTTTTTTGATTATCTACAAAGTTTCAAAGAATCTTTATTAATATTTAAACAATATTTAAAAGAACTTTAA", "aa": "MLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL", "aa_hexdigest": "dcdbed1e550841e45b35f9fdb5193f66", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 911601, "stop": 912023, "observations": { "mutation": [ 911933, 912002, 912005 ], "stop": [ 911933, 912002, 912005 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS5", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 167.0, "evalue": 1.3e-51, "gene_start": 1, "gene_end": 92, "reference_start": 49, "reference_end": 140, "reference_sequence": "MNKQKRWWMLLSYVIIGLVLLIINVVLVCCWQKGSGVFISLIGQTILVMLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL" }, "paralog": false, "identity": 0.9785714285714285, "score": 258.0, "evalue": 7.07e-90, "description": "Internal stop codon at 911933, 912002, 912005. Nonsense mutation around 911933, 912002, 912005.", "psc": { "uniref90_id": "UniRef90_C4XFS5", "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS5", "UniRef:UniRef50_C4XFS5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10704.786499999991, "isoelectric_point": 7.852652168273926 }, "id": "IGIGPGHMMC_1601", "locus": "GCA000148625_01597" }, { "type": "cds", "sequence": "contig_1", "start": 912452, "stop": 913018, "strand": "+", "frame": 2, "gene": null, "product": "Glucokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4", "UniRef:UniRef90_C4XFS4" ], "nt": "ATGGCTTTGGCAAATCACAAATATTACAAACAAAAGAAAAGTGATGTAACTCAATTTTTCACAGTATCAACAGGTTTAGGTGCTGGTCTTATTATTAATGATCAAATATATGTTGGTAAAAATCACTTAGCTCAAGAAATCGCGAGAGCACCTTTAGGTACTGATGAAGATGACTCATTTCATTTACTTCCTTATTCAGTGGAATTGTATGCTTCAGGAACAGGCATTGAATTAAGATATAACAAATTAACAGGAGAAAAATTAAACGCTAAAAAAATTTTTGCTTTATATCCAAATAATGAAAATGCAAAAAAAATAATAAATGAAGGTATAGATTCTTTAGCTAGAACTATCTCAACTTCTTTAGCTTTTTTAAATCCAAGTCTTGTTGTTTTTGGCGGTAGTGTTAGTGAACATAATAATTGGTTTATAGAAAAAGCTATTGAAAAAGCTAAAACATATACAATTAAATCTCAATATCAAAATGTAAAATTTGCTTTTGATCCTTATACAGATTTCAGCGCTGTAATGGGTTTATATTGTTTAGCAAAAGATAAATTTAAATAA", "aa": "MALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK", "aa_hexdigest": "bb9384e04443ebc48cca139096dc696e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 912152, "stop": 913018, "observations": { "mutation": [ 912206, 912371 ], "stop": [ 912206, 912371 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS4", "query_cov": 1.0, "subject_cov": 0.6394557823129252, "identity": 1.0, "score": 375.0, "evalue": 6.9e-130, "gene_start": 1, "gene_end": 188, "reference_start": 107, "reference_end": 294, "reference_sequence": "MMKIATADIGGTNTRFALFKKNKFWSKVKFETNKNNYRETLNHIAHLCNLHKIECLALCIPGPADYDKGIVLNTPNLPGWVNKNIKKYLFKHTNLKEIITENDANVMALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK" }, "paralog": false, "identity": 0.9930555555555556, "score": 577.0, "evalue": 3.35e-212, "description": "Internal stop codon at 912206, 912371. Nonsense mutation around 912206, 912371.", "psc": { "uniref90_id": "UniRef90_C4XFS4", "product": "Glucokinase", "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS4", "UniRef:UniRef50_C4XFS4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4" ], "product": "Glucokinase" } }, "id": "IGIGPGHMMC_1602", "locus": "GCA000148625_01598" }, { "type": "cds", "sequence": "contig_1", "start": 913824, "stop": 914183, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATATTTTTTGAATGGTTCTATTTTAGGTAGTACTTTATCTTTTGTTCCAATAATATCTGCTTCTTGTGTTCAAAAACAAGAAGTTCCTTTTGAAGACAAATTAATTGAATCAAAATATGAAAATAGTTCAATAATTGGCTTAAAGGACAAAGATTTACTTAAATTAAATTTAAATCAAATAAAGTATTTTATCGAGCAAACTAATAAATTAAGATTAAAGAATAATTTTAAACTAAAACAAGTTGCAAAAGAAAATGATGATCTATCTTTATTAATAAATGATAAAAAATATTCATTAAAAAGTTTTTTAAAATCAGTTAATGATAATTGGGATAAATTATCAAAAAATTGA", "aa": "MKKYFLNGSILGSTLSFVPIISASCVQKQEVPFEDKLIESKYENSSIIGLKDKDLLKLNLNQIKYFIEQTNKLRLKNNFKLKQVAKENDDLSLLINDKKYSLKSFLKSVNDNWDKLSKN", "aa_hexdigest": "b9b4fcc5ddc4ff5697e2f24068cd600c", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13791.854799999976, "isoelectric_point": 9.44856700897217 }, "id": "IGIGPGHMMC_1603", "locus": "GCA000148625_01599" }, { "type": "cds", "sequence": "contig_1", "start": 914247, "stop": 914417, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTTTGAAAATGATAATTCAGATTTTAAAAATTTAGGCTATGATAAATATGTTAAAAGATTGTTTAAATCAAAATACAATAATTTAGCAAATGCTTCTAAAAATTTACCTGATTTGCAAATGATTTTACAAGTTGCAATTTTAGGAAGTAATAGATTTTCAATTTGA", "aa": "MFFENDNSDFKNLGYDKYVKRLFKSKYNNLANASKNLPDLQMILQVAILGSNRFSI", "aa_hexdigest": "77f15913c104c6d41e9d1bf6370e39b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6535.419900000001, "isoelectric_point": 9.455336189270021 }, "id": "IGIGPGHMMC_1604", "locus": "GCA000148625_01600" }, { "type": "cds", "sequence": "contig_1", "start": 914478, "stop": 915341, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS2", "UniRef:UniRef90_C4XFS2" ], "nt": "TTGAATCAATTTAAAGAATACTTCGAAAAACAAGCAACTTTTTACTTAAAAGCCTATAAAATAGTTAATAAAGAAAATAAAGAATTTGAAAAATTAATTATTACAAGCAAGGAAAAAGAAGAGTACAAAGGCAAGTATATTTTAAAAATAAAAGTAGATTTATTGGATAAAAATAATCAAAGTATATTAAATAATGATCAAAAAAATAAAGTCTTTTATTTGACGGATTTTCAAGATTCTAATCCTAGATATCATGCATTTCAAAAATATGGAAATTTTGAAATTGATTCTTCATTAGAAACAAATGAAAAGAACAAATCTTTATTTAATGAAATGTTTAAAGAACCGACACTTTCATTTGAAAAAAATATGTTCAACTTTAATGATTTTGATTCATTGATGCACCCCTCAAAACCTTATTTAGGTTTTAATCTTAATTCTTTTATGCAATTCTATAACGCTTATAAAGAAAAGGTCAAAATTAATGTTCCTGATGAAAATTATGAAGCAAAAATAAAAGAAATAAAATTTGACAAAATATTAAATAATTCACATTCAATAGGCAAGGCTATAGTTGAAATTAAAAACAAAAAAACTAATAGAGTACAAGAAGCTAATTGGTTTACTATTGACTTTACATATGCACATAAACATATATTTGATGGCTTTTATATTCAAAATAAATTAGATTGTGATCCTTTAAAAAATGATGAATTCTTCAGCTATTATATAAATAAAGGAATTGGAACAAATAATGAATTAAAATTACCTGAAGGCATTAATGCAGAAGAATTTTATAACACTAATTTAATAGATATTGTTAACTTTATTATTTATCAAAACAGAGATTCATTTAACATTTGA", "aa": "MNQFKEYFEKQATFYLKAYKIVNKENKEFEKLIITSKEKEEYKGKYILKIKVDLLDKNNQSILNNDQKNKVFYLTDFQDSNPRYHAFQKYGNFEIDSSLETNEKNKSLFNEMFKEPTLSFEKNMFNFNDFDSLMHPSKPYLGFNLNSFMQFYNAYKEKVKINVPDENYEAKIKEIKFDKILNNSHSIGKAIVEIKNKKTNRVQEANWFTIDFTYAHKHIFDGFYIQNKLDCDPLKNDEFFSYYINKGIGTNNELKLPEGINAEEFYNTNLIDIVNFIIYQNRDSFNI", "aa_hexdigest": "f53398bb17b9c67a925477bbe4041581", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 914178, "stop": 915644, "observations": { "mutation": [ 914181, 914415, 915339 ], "stop": [ 914181, 914415, 915339 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS2", "query_cov": 1.0, "subject_cov": 0.43883792048929665, "identity": 0.986, "score": 558.0, "evalue": 5.19e-195, "gene_start": 1, "gene_end": 287, "reference_start": 233, "reference_end": 519, "reference_sequence": "MVFMSMTNISRKKKMKKYFLNRFILGSALSFAPIISASCVQKQEVPFEDKLIESKYENSSIIGLKDKDLLKLNLNQIKYFIEQTNKLRLKNNFELKQVVKENDNLFLSINNKKYSLKSFLKSVNDNWDILSKNWEIVTNKNNSLIRKGNNSTDVNVFFENDNSNFKNLGYVNYVKRLFKSKYNNLANASKNLPDLQMILQVAISGSNRFSIWKNQLSSNLLRIENIFDENAILNQFKEYFEKQATFYLKAYKIVNKENKEFEKLIITSKEKEEYKGKNILKIKVDLLDKNNQSILNNDQKNKVFYLMDFQDSNPRYHAFQKYGNFEIDSSLETNEKSKSLFNEMFKEPTLSFEKNMFNFNDFDSLMHPSKPYLGFNLNSFMQFYNAYKEKVKINVPDENYEAKIKEIKFDKILNNSHSIGKAIVEIKNKKTNRVQEANWFTIDFTYAHKHIFDGFYIQNKLDCDPLKNDEFFSYYINKGIGTNNELKLPEGINAEEFYNTNLIDIVNFIIYQNRDSFNIWNNKKMNKNSVLEIIQNKAEFEKKISTLISQYVLKFFINNKKPGYEELIKEVKVTIDPNNDYDALKYGVGKLPIRIDFINHKNEDMIKNISERKFLLGGFVGYDFSDIEAKLKTLTDADFERPLKDKTPPYLIKN" }, "paralog": false, "identity": 0.9733606557377049, "score": 950.0, "evalue": 0.0, "description": "Internal stop codon at 914181, 914415, 915339. Nonsense mutation around 914181, 914415, 915339.", "psc": { "uniref90_id": "UniRef90_C4XFS2", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XFS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS2", "UniRef:UniRef50_C4XFS2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS2" ], "product": "Lipoprotein" } }, "id": "IGIGPGHMMC_1605", "locus": "GCA000148625_01601" }, { "type": "cds", "sequence": "contig_1", "start": 915354, "stop": 915746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGTTCTAGAAATAATTCAAAATAAAGCAGAATTTGAAAAAAAAATAAGTACATTAATTTCCCAATATGTCCTTAAATTCTTTATAAACAACAAAAAACCAGGTTATGAAGAATTAATAAAAGAAGTTAAAGTTACTATTGATCCAAATAATGATTATGACGCCTTAAAATATGGCGTCGGTAAGTTACCAATTAGAATAGATTTCATTAATCATAAAAATGAAGATATGATTAAAAACATAAGTGAAAGAAAATTCTTATTAGGCGGTTTTGTTGGTTATGATTTCAGTGATATTGAAGCAAAATTAAAAACATTAACTGATGCTGATTTTGAGAGACCTCTTAAGGATAAAACTCCACCTTATTTAATCAAAAACTAA", "aa": "MNKNSVLEIIQNKAEFEKKISTLISQYVLKFFINNKKPGYEELIKEVKVTIDPNNDYDALKYGVGKLPIRIDFINHKNEDMIKNISERKFLLGGFVGYDFSDIEAKLKTLTDADFERPLKDKTPPYLIKN", "aa_hexdigest": "61d09685d3a716907af0f5a65c5049bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15118.308199999983, "isoelectric_point": 8.630850028991702 }, "id": "IGIGPGHMMC_1606", "locus": "GCA000148625_01602" }, { "type": "tRNA", "sequence": "contig_1", "start": 915782, "stop": 915871, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 68.1, "nt": "CGGTAGTTGCTCGAGAGGCTGAAGAGGTTGGTCTCGAAAACCAATAATGGTGAAAGTCATTCAAGGGTTCAAATCCCTTACTACCGGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 915858, 915860 ], "id": "IGIGPGHMMC_17", "locus": "GCA000148625_01603" }, { "type": "cds", "sequence": "contig_1", "start": 915912, "stop": 916208, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTGCCCTTAATTTCTATGGTTGGTATTGCAATTATTGCTATACCTGGTGGTATGGTAGCAGCAAGTGTTTTAACTAGTTTTCAAAAGAAAAAAGATAATAAAGAAAAAGCTAATGAGCTTGCTGAAATAATTGATGCAGAAATTGAAAGAAGAGTTAAAGAAAGAATTAAAGAAGAAGAAAAAGAAATGAAACACGATTTATTACATCGTTTTGATGAAACAATTCAAAAAGAAAAAGAATTAAAACACACAAACGAACACGCATCTCATGATCATAAAGATCATAAATAA", "aa": "MIVPLISMVGIAIIAIPGGMVAASVLTSFQKKKDNKEKANELAEIIDAEIERRVKERIKEEEKEMKHDLLHRFDETIQKEKELKHTNEHASHDHKDHK", "aa_hexdigest": "2fe14755f45989b77cb56ff893a83c18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11317.926199999987, "isoelectric_point": 6.377187156677246 }, "id": "IGIGPGHMMC_1607", "locus": "GCA000148625_01604" }, { "type": "cds", "sequence": "contig_1", "start": 916416, "stop": 916685, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTACAAGCGGCAATATTATTTTGATTCTTACATTTTTGCCTTCACTTTATGTGATTAATAATTTCTTGCCTGAAGCTAATCAAAGTAATGCATTAAAAGCTTTTAACGGCTTTAAGTTTTTAAGAATTTTAAGATTAATTATGCTTTTAAATGTTTTTGTAGTTTTTAAAAATATTTCAGAATCTGTATCAAATGAAAAAGTTTTGTTTTTCAATATCTTCTTATTTATAGGTATTTTGATCGTTTTATTTGCTCTTACAGTTTGA", "aa": "MITSGNIILILTFLPSLYVINNFLPEANQSNALKAFNGFKFLRILRLIMLLNVFVVFKNISESVSNEKVLFFNIFLFIGILIVLFALTV", "aa_hexdigest": "155106bb445b572ee9f6aef75110c8be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10128.15609999999, "isoelectric_point": 9.819711494445798 }, "id": "IGIGPGHMMC_1608", "locus": "GCA000148625_01605" }, { "type": "cds", "sequence": "contig_1", "start": 916914, "stop": 917039, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAGAAACTAACTTAATAAAAAATAAGAAAATAAATGACATTTTTGAAGCAATTGGAATAATTGTTACAGTTGATAACGATTTAAAAACAAACAACAAAAAACATAGAATTACATTATGA", "aa": "MERETNLIKNKKINDIFEAIGIIVTVDNDLKTNNKKHRITL", "aa_hexdigest": "de5678d6c1aca33bd754db253ab59e4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4794.5322, "isoelectric_point": 9.301836967468262 }, "id": "IGIGPGHMMC_1609", "locus": "GCA000148625_01606" }, { "type": "cds", "sequence": "contig_1", "start": 917368, "stop": 917514, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACATAATTAATACAGCTCGTGGTTTCTTAGAAAAATATCAAGAAGATGCTTTATTTAAAGAATGTATTTTGAATAATGCTGATGCTAGGGATGAAATTTTTGCTTTTGGTTTTTTAAGAGATACAAGAAGCAATTCGTGA", "aa": "MSNIINTARGFLEKYQEDALFKECILNNADARDEIFAFGFLRDTRSNS", "aa_hexdigest": "a403fecf85cf8ba49e1f15ef2b3a0434", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5546.124199999998, "isoelectric_point": 4.7658586502075195 }, "id": "IGIGPGHMMC_1610", "locus": "GCA000148625_01607" }, { "type": "cds", "sequence": "contig_1", "start": 917648, "stop": 917893, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAGATGAATCTACCAAATAAACTAACAATACTTAGATTGATTTTATTCTTACCTCTTTTAGCTCTTTTTATTACTTATAAGATGATCCAAAATTACATGGATAATTACTATAAAATAGCTGGCCGTTCAATATTATCTGGAATTTTACTTATCTTTTTAGGAGCAATGATTACTGACTATTTTGATGGCAAAATTGCTAGAAAATACAATATGATTACAAGCTTTGGAAAGCTGTGA", "aa": "MTKKMNLPNKLTILRLILFLPLLALFITYKMIQNYMDNYYKIAGRSILSGILLIFLGAMITDYFDGKIARKYNMITSFGKL", "aa_hexdigest": "698cabfcd0d8447851f1d1cf11f03082", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9417.429299999993, "isoelectric_point": 9.99648380279541 }, "id": "IGIGPGHMMC_1611", "locus": "GCA000148625_01608" }, { "type": "cds", "sequence": "contig_1", "start": 918068, "stop": 918289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCTTGTTTCAAACGCTTGCAATTATTGCTATTTTAATTATTGCTATAAGCTTTGAATATCCTATTGAATTGGTTGAAAAATATGGCCTAACTTTAGTTCAAAGTGATGCTTGTATTTATGCAATTAACACTCTTACAATTGTTGCATTATTCTTCAGTATTTACTCAGGAATATTGTATGTTAAAAAAATAATCCCTTTCCTAGAAATGGTATAA", "aa": "MKTLFQTLAIIAILIIAISFEYPIELVEKYGLTLVQSDACIYAINTLTIVALFFSIYSGILYVKKIIPFLEMV", "aa_hexdigest": "1da5b8764598b52dae3e7412251de1b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8249.945299999998, "isoelectric_point": 5.002422523498535 }, "id": "IGIGPGHMMC_1612", "locus": "GCA000148625_01609" }, { "type": "cds", "sequence": "contig_1", "start": 918328, "stop": 918615, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355028.1", "SO:0001217", "UniParc:UPI0001E3305D", "UniRef:UniRef100_C4XFR8", "UniRef:UniRef50_C4XFR8", "UniRef:UniRef90_C4XFR8" ], "nt": "ATGAATAAAGATGACAAAAAAGAAAACAGCTTAGAGATTACAACTCTATACGATGCTGAAAAATTTAGAATTATTAAGAATGAAGATTTAAACAATTTAACTAATGAACAAAGAGAAAAATTAGTTAAAGATGCAATTGATAATGCTAAAAAAAATGAAGCCAATATAATTGCAAATAGGAAGAAAAGATATTTGTCTTCTTTAGCTGTAATTTTAGTAGCTGGAATAATCATTGCAATTACAGTAATTTTGATACTTGCACTAATTATTTACTTACATAAAAAATAA", "aa": "MNKDDKKENSLEITTLYDAEKFRIIKNEDLNNLTNEQREKLVKDAIDNAKKNEANIIANRKKRYLSSLAVILVAGIIIAITVILILALIIYLHKK", "aa_hexdigest": "b47f11197ed18bc1e6b5968d894f0d86", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3305D", "ncbi_nrp_id": "WP_013355028.1", "uniref100_id": "UniRef100_C4XFR8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305D", "RefSeq:WP_013355028.1", "UniRef:UniRef100_C4XFR8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR8", "uniref90_id": "UniRef90_C4XFR8" }, "psc": { "uniref90_id": "UniRef90_C4XFR8", "uniref50_id": "UniRef50_C4XFR8" }, "pscc": { "uniref50_id": "UniRef50_C4XFR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10891.683399999989, "isoelectric_point": 9.362372779846194 }, "id": "IGIGPGHMMC_1613", "locus": "GCA000148625_01610" }, { "type": "cds", "sequence": "contig_1", "start": 918650, "stop": 919072, "strand": "-", "frame": 1, "gene": null, "product": "NAD-dependent protein deacylase", "db_xrefs": [ "EC:2.3.1.286", "GO:0034979", "GO:0051287", "GO:0070403", "SO:0001217", "UniRef:UniRef50_C4XFR7", "UniRef:UniRef90_C4XFR7" ], "nt": "TTGGATATTAAAGCTGGTAATAAATATGTAATTGAATTACATGGAAATATTAATCGTAATTATTGTTTGCTCTGTGGTAAGTTTTATGATTTAGCTCAATTAATTCATCAAAAAGATAAAGATGGAATTCCTACTTGTAAATGTGGGGGAGTAATAAATCCAGATGTAGTTTTATATGAAGATCCACTAATGGAAGATTCAACTAATGATGCAGCTGAAGCCATTTCAAATAGTGATTTATTAATTATTGCAGGTACTTCATTAAGTGTTTATCCTGCAGCTTCATATATTCATTTTTATCAGGGTAAAAGAATAGTAATTCTAAATAAAGATACTTCACGTTATGAAAATAGTAACAATGAAAATATCTTGTTGTTTAATGAAAATATGAAGGATGTTTTTGAAAATTTAGCTAATTCATAG", "aa": "MDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS", "aa_hexdigest": "92cf2e5d29258527de8c46031b4582df", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 918650, "stop": 919372, "observations": { "mutation": [ 919120, 919216, 919363 ], "stop": [ 919120, 919216, 919363 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR7", "query_cov": 1.0, "subject_cov": 0.5577689243027888, "identity": 0.993, "score": 281.0, "evalue": 1.52e-94, "gene_start": 1, "gene_end": 140, "reference_start": 112, "reference_end": 251, "reference_sequence": "MVQNFNEKIDKFKNWIKESKHIVFFSGAGVSTASGIPDFRSADGLYSKKFKNMNPESILSRSFWRKNKKDFYEYYFSNIAFDNIKPNIIHETVASWCNKNKCHVITQNIDNLDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS" }, "paralog": false, "identity": 0.9875, "score": 480.0, "evalue": 2.55e-175, "description": "Internal stop codon at 919120, 919216, 919363. Nonsense mutation around 919120, 919216, 919363.", "psc": { "uniref90_id": "UniRef90_C4XFR7", "product": "NAD-dependent protein deacylase", "ec_ids": [ "2.3.1.286" ], "uniref50_id": "UniRef50_C4XFR7", "go_ids": [ "GO:0034979", "GO:0051287", "GO:0070403" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR7", "EC:2.3.1.286", "UniRef:UniRef50_C4XFR7", "GO:0034979", "GO:0051287", "GO:0070403" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR7" ], "product": "protein acetyllysine N-acetyltransferase" } }, "id": "IGIGPGHMMC_1614", "locus": "GCA000148625_01611" }, { "type": "cds", "sequence": "contig_1", "start": 919248, "stop": 919436, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAATTTTTTAGAATATAAACCATCAGCTGATCTAAAATCAGGAATTCCTGAAGCTGTTGAAACACCAGCTCCACTAAAGAAAACAATATGTTTAGATTCTTTAATTCAATTTTTAAATTTGTCTATTTTTTCATTAAAATTTTGCACCATATACCTCTATTTAATTTTAGCAAAAATATAA", "aa": "MFLNFLEYKPSADLKSGIPEAVETPAPLKKTICLDSLIQFLNLSIFSLKFCTIYLYLILAKI", "aa_hexdigest": "c841bf6945028a5052c8f73c8ce29cab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7041.417999999996, "isoelectric_point": 7.743994331359863 }, "id": "IGIGPGHMMC_1615", "locus": "GCA000148625_01612" }, { "type": "cds", "sequence": "contig_1", "start": 919715, "stop": 920026, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4", "UniRef:UniRef90_A0AB32XDE4" ], "nt": "ATGAGATTAAGAAATGACAAAGATGCACCAAAAAAGCTTGAAGAATCTAATTTATTGATTACTAAAAGTCAATATCCTATTAGCTTAAAAAATACTGATGTAATTGAAATTGGCATGGGTAAAGGCGAAATGATAACTCAATTAGCTGCTCTAAATCCAAATATAAATTTTTATGGTTTAGAAAAGTTTGCTACTGTAGCTGCTAAAAGCGTAAGAAGAGCTAAAGAATATAAGCTTAATAACTTTAAAATTATTTTAGAAGATGCTAAAGAATTAGAAAGCATTTTTAAAGGAACTTGTGGCACAATTTGA", "aa": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELESIFKGTCGTI", "aa_hexdigest": "215ef0b37058954a1aceccabe1d106ab", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 919606, "stop": 920026, "observations": { "insertion": [ 919645 ], "mutation": [ 919696, 919717 ], "stop": [ 919696, 919717 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDE4", "query_cov": 1.0, "subject_cov": 0.7410071942446043, "identity": 0.99, "score": 199.0, "evalue": 4.46e-64, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELKSIFKGTCGTIWLTFSDPWPKARHFKRRLTYKDFLKNINLLWIKILF" }, "paralog": false, "identity": 0.9784172661870504, "score": 263.0, "evalue": 1.07e-91, "description": "Internal stop codon at 919696, 919717. Frameshift due to insertion around 919645. Nonsense mutation around 919696, 919717.", "psc": { "uniref90_id": "UniRef90_A0AB32XDE4", "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDE4", "UniRef:UniRef50_A0AB32XDE4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" } }, "id": "IGIGPGHMMC_1616", "locus": "GCA000148625_01613" }, { "type": "cds", "sequence": "contig_1", "start": 920134, "stop": 920328, "strand": "+", "frame": 1, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355031.1", "SO:0001217", "UniParc:UPI0001E3305F", "UniRef:UniRef100_C4XFR5", "UniRef:UniRef50_C4XFR5", "UniRef:UniRef90_C4XFR5" ], "nt": "ATGGAATTTAAAGACAAAATTTTACGTGCTAGAGCTCTTTTAAACTTAACTCAAGAAGAATTAGCAAAACAACTTGGTGTTTCTTTTAACACCATAAATAGATGGGAAAATGGAAAAGTTAAGCCAACAAAAGTTGCTAAATTGAGATTTGATGAATATTGTAGAAAAAATGGTGTTTTGTTGGAGGTTAGATAA", "aa": "MEFKDKILRARALLNLTQEELAKQLGVSFNTINRWENGKVKPTKVAKLRFDEYCRKNGVLLEVR", "aa_hexdigest": "22909f2efd67cb793c157f1fa02e3864", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E3305F", "ncbi_nrp_id": "WP_013355031.1", "uniref100_id": "UniRef100_C4XFR5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305F", "RefSeq:WP_013355031.1", "UniRef:UniRef100_C4XFR5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR5", "uniref90_id": "UniRef90_C4XFR5" }, "psc": { "uniref90_id": "UniRef90_C4XFR5", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_C4XFR5" }, "pscc": { "uniref50_id": "UniRef50_C4XFR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR5" ], "product": "HTH cro/C1-type domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1617", "locus": "GCA000148625_01614" }, { "type": "cds", "sequence": "contig_1", "start": 920328, "stop": 920801, "strand": "+", "frame": 3, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "nt": "ATGGAATTTAATGAAGATACAAGAGTAAAATTACCAGCAACGATTCACTTTTTAAAACTTGGTTACAATTATCAAACTGTTAGAAGCCCAGAAATTAATAGAGACAATAACATTTTTGAAGAAAGATTTCATAGAGCAATTGAAAAAATAAATAAGAAAAAATTTGCTAAAGAAGAAATAAATGAATTGATCTCTGATATAAGCAGAAAAATCAAAAATAAGGATTTAGGAAAAGAATTTTATAATCGATTAATTTCGACCGATTACCCTATAAAACTGATAGATTTTGAAAATATTGAGAACAATGATTTTGCAATTGTTAATGAATTAACTTTTAGAAGTAACGCTAATTCTAATTATGATTCATTTAGACCAGATATTACAATTTTAGTTAATGGTATGCCATTAGCATTTTTAGAAGTAAAAGTTAAAAACAACAAAAATGGTATTCAAGCAGAATTTATTAGAATGTGA", "aa": "MEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRM", "aa_hexdigest": "5a44f6c11b8f9e40968808b5677e8983", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_R5XCY4", "query_cov": 0.9872611464968153, "subject_cov": 0.9935897435897436, "identity": 0.568, "score": 167.0, "evalue": 1.4e-50, "valid": false, "product": "type I site-specific deoxyribonuclease", "uniref50_id": "UniRef50_R5XCY4" }, "pscc": { "uniref50_id": "UniRef50_R5XCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "product": "type I site-specific deoxyribonuclease" }, "genes": [], "id": "IGIGPGHMMC_1618", "locus": "GCA000148625_01615" }, { "type": "cds", "sequence": "contig_1", "start": 920844, "stop": 921092, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATTGTTTCATTTACAAACAATATGGATTATGAAGAATTTAATGATGCTGAGGCTTCACTACTAAAACAAGGCTCTTTCTATACAACACCAAATGGAAAAGACAATACTACTTTTTCAGCTTTTAGAGAAGATGAACCTAACTACCATGCTAACTACTCTTTTGAGGAAATAGACAATGACAAAATAAAAGAAATTTTAATTGATAATAATATTAAAACTTCACATGTTGATAGTGATTGA", "aa": "MLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSD", "aa_hexdigest": "ef0950a75bc7fd9a519371e59a491703", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9492.098799999992, "isoelectric_point": 4.251465797424316 }, "id": "IGIGPGHMMC_1619", "locus": "GCA000148625_01616" }, { "type": "cds", "sequence": "contig_1", "start": 921186, "stop": 921347, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTATCTTAGCCAAATAAAAGAAATTAAAAATGAAACTTTAAATAAAGTTGAACAATTGCCAATTAATCAAAAACACATTATGAGATATCCTCAATTTTTCGCTACAAGAGCAATTATCAAAAGATTCAAACGAACTAATAAAAATGGAATTATTTGA", "aa": "MMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGII", "aa_hexdigest": "5b21485bac4e6ec319f5f9f95330c7c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6436.6222, "isoelectric_point": 10.509910011291506 }, "id": "IGIGPGHMMC_1620", "locus": "GCA000148625_01617" }, { "type": "cds", "sequence": "contig_1", "start": 921468, "stop": 922118, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACAAGCTTCTGCAGAATTTGCCAATAGAGGTTTCAATGTTGTTAATGTTGATTCAAAAGAAGCATTCGCTAAAGAATTAAATGAGACAATCGATAGAAATAATAATGGTATTGGCACAATTTGTGTTGTAAACATTCATAAATTTATGGATAGTTCAAAAATGCCAGAAGCTAAAAATGATTACAACATAAGAACTCAAAGAATTTTCTTTATTGATGAAGCTCACAGAAGTTATACAATGCATGGTGAATTTTACAAAAACCTTATGACTTGTGATAGAGATGGAATTTACATTGCAATGACTGGTACACCTTTATTAACTAAAAGAGAAAGATCAAATTTAAAATTTGGAGACTACATTCATAAATACTTTTATGATAAATCAATAGCTGATGGTTATACTTTGAGAATTAAAAAAGAACAAATTAAAACTGAAGCTAGAGCTACAATTATTAGAAACTTAAATATTGAAATTGAAAAATTAGAATCAAGAATTATTTATGAATCAGATGATTATGTTAAAAGCGTTAGCCAATATATAGAAAAAGACTTTATTGATTTTAGATTTATTTGCAGAAATGATTTAACAATTGGTGGAATGATTGTCTGTCGTTCAAATGACCAAGCGAAAAAAATTCATGAATGA", "aa": "MRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHE", "aa_hexdigest": "dc1ee2318cc28d86bb1673fd40c0666c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25291.487800000028, "isoelectric_point": 8.23810863494873 }, "id": "IGIGPGHMMC_1621", "locus": "GCA000148625_01618" }, { "type": "cds", "sequence": "contig_1", "start": 922176, "stop": 923423, "strand": "+", "frame": 3, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01153", "SO:0001217", "UniRef:UniRef50_C4XFR4", "UniRef:UniRef90_C4XFR4" ], "nt": "TTGCAAGACAACATAAATAAAAATAATCAAAAAACCTTTAAAGAAAGTAATTATCCAGATATTTTAGTTGTTCACTATATGCTTACAACTGGATATGATGTTAAAAGACTTAAGAAGATGTATTTATTAAGAGGACCTAAAGCTCAAAATCTTTTGCAAACTATTTCACGTGTTAATAGACCCTATCGTAGTCCTAATGGCCAAGAATATCAATATGGATATATAGTTGATTTTATTGATATTGAAAAAGAATACAACAATACTATTGATGCTTATCGTAAAGAAATAGAAGAAGAATACGATTTTGAAGAAGAAGACACTTCCCTTTCTGGTTTAATTATTGATAAAACAGATATCAAAAAAAGATATGATGTTTTAAAAAGTGATCTTGAAAAATTTGTTAATAATTACAATTTAGAAACCTTTGTTGACAAAATTAACAATTTTGACAAAGATACTTTACAAAAGATCAAAAAGATTCTTGTTAATATTAAAAACTGTTCAATTGAATTCAAATTATCACAAGCTGATGAATATTCAAAACTCATTAACGATTATGAAATCAATGTGTTTATTAAAGAAGTACAAAAGAAAATTAATTTTTTAAACTTGCGTTCAGATCCTATTGATACAATTCAATTAATAGATAACAAAGAAGTTGTTCAAATCATTTATGATTTTCTTTTAAAAAATATTACTGTTTTAGATTTAGGTAAATTTGATAAAGAAAATAAAATGGAACTTATTAAACAAAAGTTTATGAAAATGCAAAAAGAAATTAAAGAAAACAAAAATACAAGAGATGTTAAATTTATGAATCTTAATGCATTGATTAAGAAATTACTTGATAAATTAGCTGTTGAGGAATTGACTTACGAAGAAGTAGAAGATGAATTAGATTTAATTTACAATTCAACTAAAAAAATAAATGAAGAAAATAAAGAAATATCTAAAGACTTTGGAAATAGTTTGGCTTTTGTCACAACAATGAATATTGTTTTAGCAGAAACTAAATTAGATCGTTCAATTTTAAAGAACTTTTTATTAATGGTTTATGATGAATTAAAAGGCAACATTTCTGACGAAATCCTCATTAAACAAGGCAAAAATGGATTTATTGACTCAACAAAATCTAAAATAACAACTAGATTAATTAAGAAGAATTTATATAAACAAATTAAAGATAATTACGATTATATTTTGAACGAATTATACTTTAATCTGCTTAATTACAAGGAAACACTTTAA", "aa": "MQDNINKNNQKTFKESNYPDILVVHYMLTTGYDVKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL", "aa_hexdigest": "8b530b40119e903f3b5f7ad67f09757f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 921876, "stop": 923423, "observations": { "mutation": [ 922116 ], "stop": [ 922116 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR4", "query_cov": 1.0, "subject_cov": 0.4021317829457364, "identity": 0.998, "score": 789.0, "evalue": 2.5e-278, "gene_start": 1, "gene_end": 415, "reference_start": 618, "reference_end": 1032, "reference_sequence": "MMEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRMWEDRLKVDEYRKFFNMLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSDWFQTNLSDTTPCNRFITSIFDKERFLYFIKYGMMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGIIWHTQGSGKTALSAFATKVIKDYWSKKKINARFFFIVDRLDLMRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHEWFKKNSKLTTGLVLSDTDNPLQDNINKNNQKTFKESNYPDILVVHYMLTTGYDVKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL" }, "paralog": false, "identity": 0.9980582524271845, "score": 1008.0, "evalue": 0.0, "description": "Internal stop codon at 922116. Nonsense mutation around 922116.", "psc": { "uniref90_id": "UniRef90_C4XFR4", "product": "type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR4", "kegg_orthology_id": "K01153", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR4", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR4", "KEGG:K01153" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR4" ], "product": "type I site-specific deoxyribonuclease" } }, "id": "IGIGPGHMMC_1622", "locus": "GCA000148625_01619" }, { "type": "cds", "sequence": "contig_1", "start": 923432, "stop": 924436, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "nt": "ATGACTAATTTTACAAAACTTGAAAATAATATAAAATCAATAATCGATGATTTGAAGGGATTGTGTTCAACAAATGGTCTTTCAAATACAGCTGGCGAAGAAATAGTTGTTACTTCTGTCTTTTTATATAAATTTCTAAATGATAAATTCATTTCAAATTTAAAAAAATTTGCAAAAGAATTAAATGTTCCTTTTGAAGAAATAATTAAAAATTCAAACTATATGGATGGATTTTATGATGCTTATCCACAAGATGTTGCATTCAAATATGAAGATACAATTGATTATTTAGTTGGATACATTAATGAAGATGATTTTTATCAAAAATTTGACGATACATTAATTAGAATTTCAGATTATAAACAAAATGAAGCTTTTAATGTTGATACTGCAGAAGGAACTAAAAAGCCACTTTTTGTTCGTCTTAGTACTTTTGTTGAACCTTCAAAAAGAAATAACTTCACAAAAAATGTTTTTTCTTACATTACAAGAGATAGATTTGATTTTGGTGAAGCTTTTAATAATAACTATGATTTCTTTTCAACTATTTTTGAATACCTAATTTCAGATTACAACATTGCAAGTGGTAAATATGCTGAATACTTTACACCTCAAACATTATCAAAAGCTATTGGTGAAATATTAGTTAAAATGTCTCCAATTGAAGATAAAATTTATGAAATATATGACCCAAGTGCAGGTTCTGGATCGCTTGTGTTGCATTTAGCAAATGAACTTGGAGAAGGTAAATTTGGAAATAAGGCTCGTGTTTACACTCAAGATATTTCACAAAAATCATCAAGATTTTTAAGAATAAATATGTTACTTAATGGTTTAAAAGAATCACTAGATAACATAATTGAAGGCGATACCTTACTTACCCCAACACACTATAAAAAAGCAGGAGACGCAACAACAGGTCTTAAACAATTTGATTTTATTACATCTAATCCACCATTCAAAACTGACTTCTCTTCTACTAGAGATAACATTGAATTAATGTGA", "aa": "MTNFTKLENNIKSIIDDLKGLCSTNGLSNTAGEEIVVTSVFLYKFLNDKFISNLKKFAKELNVPFEEIIKNSNYMDGFYDAYPQDVAFKYEDTIDYLVGYINEDDFYQKFDDTLIRISDYKQNEAFNVDTAEGTKKPLFVRLSTFVEPSKRNNFTKNVFSYITRDRFDFGEAFNNNYDFFSTIFEYLISDYNIASGKYAEYFTPQTLSKAIGEILVKMSPIEDKIYEIYDPSAGSGSLVLHLANELGEGKFGNKARVYTQDISQKSSRFLRINMLLNGLKESLDNIIEGDTLLTPTHYKKAGDATTGLKQFDFITSNPPFKTDFSSTRDNIELM", "aa_hexdigest": "871ae26b538784c51a21bc31cda63678", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378PT86", "query_cov": 0.8083832335329342, "subject_cov": 0.9926470588235294, "identity": 0.6779999999999999, "score": 379.0, "evalue": 1.44e-129, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A378PT86" }, "pscc": { "uniref50_id": "UniRef50_A0A378PT86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "IGIGPGHMMC_1623", "locus": "GCA000148625_01620" }, { "type": "cds", "sequence": "contig_1", "start": 924497, "stop": 924643, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTATCTTTTATTTATTCAACATATTCTTTATTCATTAAAAGAAGGTGGTAAAGCCGCTATAGTTGTACCTACAGGGTTTATTACAGCACAAAGTGGAATTGAAAAAACTATTAGACAATATATTATTGATAGAAAATGA", "aa": "MAIYLLFIQHILYSLKEGGKAAIVVPTGFITAQSGIEKTIRQYIIDRK", "aa_hexdigest": "c27f2ab7268c6135ce0229476ee18b4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5409.3904999999995, "isoelectric_point": 9.600454521179199 }, "id": "IGIGPGHMMC_1624", "locus": "GCA000148625_01621" }, { "type": "cds", "sequence": "contig_1", "start": 924662, "stop": 925087, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATCAAATATTTTTGCTAATACTGGAACTAACGTATCCGTTTTATTTATAGATAAAACAAATACTAATGGTGAAGTGTTATTAATGGACGCTTCAAAAATGGGTCATAAAGAAAAAGTTAAAGATCTTCAAAAAACAGTTTTAACTCATGATGAATTAAACAAAATAGTTAATGATTTTGTTAATCATAAAGTTGAAGATGATTTTACAATTTCAGTTACCTATGATCAAATAAAAGAAAGAAACTATTCATTATCAGCTGGTCAATATTTTGAAGTAAAAATTGAATATGTTGATATTACTCAAGAAGAATTTGACAAAATGATTGATGACTTTAATAAAGAATTAGAACAATTTACAAAAGATACAGAAGAATTCCAAAAAGAGATGTCTAAAACATTGAAGGAGTTGAAGCTAAAATAA", "aa": "MPSNIFANTGTNVSVLFIDKTNTNGEVLLMDASKMGHKEKVKDLQKTVLTHDELNKIVNDFVNHKVEDDFTISVTYDQIKERNYSLSAGQYFEVKIEYVDITQEEFDKMIDDFNKELEQFTKDTEEFQKEMSKTLKELKLK", "aa_hexdigest": "2384f6fe1c63022b714c5656a9d56b25", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16470.382100000003, "isoelectric_point": 4.8395788192749025 }, "id": "IGIGPGHMMC_1625", "locus": "GCA000148625_01622" }, { "type": "cds", "sequence": "contig_1", "start": 925104, "stop": 925220, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAAATTAAAAGAATTAGGTATATTTGAAACTGGAAGCACATTAATCAAAAAAATTGGAAATTTTCCTGCTTTTGGAGGCAATGGCATTATTACATATGTGAATAAATGA", "aa": "MEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNK", "aa_hexdigest": "1b63389fc682220f1b547564c3e65f25", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.872100000002, "isoelectric_point": 9.224861717224119 }, "id": "IGIGPGHMMC_1626", "locus": "GCA000148625_01623" }, { "type": "cds", "sequence": "contig_1", "start": 925344, "stop": 925586, "strand": "+", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR2", "UniRef:UniRef90_C4XFR2" ], "nt": "ATGGCAAGATTTTATTTTTATTTTCTTTTACATTTAAACTTAAATAAATTTCATATAGGTTCTTCACAGCCTTTATTAACTCAAGGAATTTTAGGAAACATAAAAATAAATATTCCTAGTTTAAATAAGTGCCAAAAAATATCAAAAATATTAGATAACATAGATAATCAAATTGAGCGAAATAATTCTATGGTTCAAAAGTTACAGTGTTTTGAGCAAGCATTAATTTTCTCAAAAAAGTGA", "aa": "MARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQCFEQALIFSKK", "aa_hexdigest": "d571f75676aa5c83c0cc452078ef3dfa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 925089, "stop": 925607, "observations": { "mutation": [ 925218, 925584 ], "stop": [ 925218, 925584 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR2", "query_cov": 1.0, "subject_cov": 0.46511627906976744, "identity": 0.988, "score": 152.0, "evalue": 9.12e-46, "gene_start": 1, "gene_end": 80, "reference_start": 86, "reference_end": 165, "reference_sequence": "MIRINMEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNKWNVDEDAIIIGRVGANCGCVNITNKKSFVTDNALIFKPKEKNMARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQSFEQALIFSKKWGANYAC" }, "paralog": false, "identity": 0.9767441860465116, "score": 337.0, "evalue": 9.25e-121, "description": "Internal stop codon at 925218, 925584. Nonsense mutation around 925218, 925584.", "psc": { "uniref90_id": "UniRef90_C4XFR2", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR2", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR2", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR2", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR2" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "IGIGPGHMMC_1627", "locus": "GCA000148625_01624" }, { "type": "cds", "sequence": "contig_1", "start": 925597, "stop": 925722, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826", "UniRef:UniRef90_UPI000FF44826" ], "nt": "ATGCTTGTTAAATTGAAAGATATTGTAACTTTCATTAATGGAAGAGCCTATTCTCAACCTGAATTACAAGACAAGGGAAAATATAGAATAGTGCGAGTAGGAAATTTTTCTGGTAAAAATGAATGA", "aa": "MLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNE", "aa_hexdigest": "7a0be707c383d09657e1b0ce1df1f77d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 925597, "stop": 925851, "observations": { "mutation": [ 925720, 925786 ], "stop": [ 925720, 925786 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44826", "query_cov": 1.0, "subject_cov": 0.4880952380952381, "identity": 0.976, "score": 83.2, "evalue": 4.73e-20, "gene_start": 1, "gene_end": 41, "reference_start": 1, "reference_end": 41, "reference_sequence": "MLVKLKDIVTFINERAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHI" }, "paralog": false, "identity": 0.9642857142857143, "score": 170.0, "evalue": 7.76e-57, "description": "Internal stop codon at 925720, 925786. Nonsense mutation around 925720, 925786.", "psc": { "uniref90_id": "UniRef90_UPI000FF44826", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44826", "UniRef:UniRef50_UPI000FF44826" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826" ], "product": "restriction endonuclease subunit S" } }, "id": "IGIGPGHMMC_1628", "locus": "GCA000148625_01625" }, { "type": "cds", "sequence": "contig_1", "start": 925885, "stop": 926685, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR1", "UniRef:UniRef90_C4XFR1" ], "nt": "ATGTTTCTTTATTATCTTTTAATGTATATGACACCTTATTGGCTTTCATCAACTAATGGATCAATAATGATTCATATTACAAAAGAAACAATGGAAGAAAAAATTGTTGATTTGCCTCCACTAAAAACTCAAAAAAAGATTTCTAAAATATTAGAAAATTTAGACAAGCAGATTGAAAAGAATTTGCATATAGTCAAAAGATTACAAGTTATGGGACAGGCTATTTTTGATATGTTTTTGAATAATGCGAAAGATTATGAAAATATAGAAAGTTTGTGCAAAATAATTTGGGGTCAGTGTCCAAAAGGAAACAACATTTTAAGCGAAAACGTATCAAATAATTTAATGCTTTATGCATCAGGAGCAGGTGATTTAGAGAATAATAAAATATTAATTTCTCCTAAAGCTTTTACTGATAAACCAATTAAAATAATAGATAATAGGACAATTTGTATGTCTATTGCGGGAACTGTTGGTAAAATTGGAATAAGTGATAAAAATATTGCAATTGGGAGAGCTATGGTTGGATTTTATAATGAAAAAAAATTTGGACTAATTTATTTTATTCTAAATAAATATAGTTCATTTTTAAAAAGACAATCAATTGGAGCTATACAAAAAATTATTAACAAAAATCATCTCAATATTGTGAATGTTCCAATATTAACAAACGAAAAAAACAATTTATTAAACGAATTAATAACTAAATGCATGAAATTAGAAAAAAATACTTTAAGTTTGATAAAGCTTAAAGAAAAGTTGATACCTTTATTAATAAATGGCCAAATAGAAATAAATTAG", "aa": "MFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN", "aa_hexdigest": "2772fdf5fd13c481cc24e4e0aff158a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 925594, "stop": 926685, "observations": { "mutation": [ 925720, 925786, 925849, 925861 ], "stop": [ 925720, 925786, 925849, 925861 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR1", "query_cov": 1.0, "subject_cov": 0.7327823691460055, "identity": 1.0, "score": 508.0, "evalue": 3.92e-180, "gene_start": 1, "gene_end": 266, "reference_start": 98, "reference_end": 363, "reference_sequence": "MMLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHIWKIKWDEKRVDKMFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN" }, "paralog": false, "identity": 0.9862258953168044, "score": 712.0, "evalue": 4.56e-263, "description": "Internal stop codon at 925720, 925786, 925849, 925861. Nonsense mutation around 925720, 925786, 925849, 925861.", "psc": { "uniref90_id": "UniRef90_C4XFR1", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR1", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR1", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR1", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR1" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "IGIGPGHMMC_1629", "locus": "GCA000148625_01626" }, { "type": "cds", "sequence": "contig_1", "start": 926709, "stop": 927086, "strand": "-", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR0", "UniRef:UniRef90_C4XFR0" ], "nt": "TTGAAAAAAGGTACTGTAGTTATTTCTATAACAGGAAATATAAGGGTCAGTTATCTTGCAATTGATTCATGTATAAATCAATCTATAGTAGGAATAGAAGAAAATGAGTTATTGAAAATAGGATATTTATATCCTTTTTTAAAGAATAAAATTGAGTTTTTAATAAGATCTTCTACTGGGAACTGTCAAAAACATATAAATAAAAATTTTATAGAAAATTTAAAAATTGTTTTACCACCAAAAAATGTTTTGGACATATTTAATAATTTAACTCAAAATATATATGCTAAAATAAGTCAAATCAGTTTAATGACTAAGAAATTAATAAAATTCAAAAATAAGCTATTACCTTTATTGATTAATCAACAAATTATTTAA", "aa": "MKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII", "aa_hexdigest": "f1bf1e76eae9959ca484347e32675609", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 926709, "stop": 927386, "observations": { "mutation": [ 927179, 927197, 927272, 927353 ], "stop": [ 927179, 927197, 927272, 927353 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR0", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.992, "score": 237.0, "evalue": 1.09e-77, "gene_start": 1, "gene_end": 125, "reference_start": 101, "reference_end": 225, "reference_sequence": "MQVMGQAIFNRWFLQFEHFKKDNKFKYNEDLNLKIPENWEVKKIAEICKIFLGGTPSTKNREYWNGEINWLNSGEVANFPIIDSEKTINEKGLKNSNTKLLKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII" }, "paralog": false, "identity": 0.9777777777777777, "score": 430.0, "evalue": 4.92e-156, "description": "Internal stop codon at 927179, 927197, 927272, 927353. Nonsense mutation around 927179, 927197, 927272, 927353.", "psc": { "uniref90_id": "UniRef90_C4XFR0", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR0", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR0", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR0", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR0" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "IGIGPGHMMC_1630", "locus": "GCA000148625_01627" }, { "type": "cds", "sequence": "contig_1", "start": 927396, "stop": 927968, "strand": "-", "frame": 3, "gene": null, "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "db_xrefs": [ "KEGG:K14060", "RefSeq:WP_013355037.1", "SO:0001217", "UniParc:UPI0001E33078", "UniRef:UniRef100_C4XFQ9", "UniRef:UniRef50_C4XFQ9", "UniRef:UniRef90_C4XFQ9" ], "nt": "ATGATTTATGGTTATACAAGAGTTAGCACAAAAAAACAAATTGATGGATATGGTTTTGAAATACAACAAAATGAAATATTGAATAAATATCCAAACGCAATAATAATTAATGAACAATTTACAGGAACAAAATTAAATAGACCACTATTTAATGATCTATTAACTAAATTAGAAAAAGATGATATTCTAGTAGTTTCTAAATTAGATAGATTTGCTAGAAATACAGTTGAAGGTATTAAAGTAGTTGAAGCTTTATTTAAAAAGAAAGTAGCAATACATATTTTAAATATAGGCTTATTAGAGGACACTCCAATGGGTCGTTTTTTCTTAACAACTATGTTAGCAGTTGCTGAATTAGAAAGAAACATGATAGTAGAAAGAACACAAAATGGTAAAGAGATAGCAAGACTAAGAACAGATTATAAAGAAGGTAGACCAAAAGTTTATACTGAAGATCTTTTAAGTGAAGCAATTTTGCTTTTAGATTCCTTTTCCTATCGCCAAGTTTCACTTTTAACTGGTATTAGTAAATCAACATTAGTTAGAGCTAAAAAAGCACTAAAAAATAGCTAG", "aa": "MIYGYTRVSTKKQIDGYGFEIQQNEILNKYPNAIIINEQFTGTKLNRPLFNDLLTKLEKDDILVVSKLDRFARNTVEGIKVVEALFKKKVAIHILNIGLLEDTPMGRFFLTTMLAVAELERNMIVERTQNGKEIARLRTDYKEGRPKVYTEDLLSEAILLLDSFSYRQVSLLTGISKSTLVRAKKALKNS", "aa_hexdigest": "9b58a564681be0c4a065fead940ccb60", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E33078", "ncbi_nrp_id": "WP_013355037.1", "uniref100_id": "UniRef100_C4XFQ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33078", "RefSeq:WP_013355037.1", "UniRef:UniRef100_C4XFQ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFQ9", "uniref90_id": "UniRef90_C4XFQ9" }, "psc": { "uniref90_id": "UniRef90_C4XFQ9", "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XFQ9", "kegg_orthology_id": "K14060" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ9" ], "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1631", "locus": "GCA000148625_01628" }, { "type": "cds", "sequence": "contig_1", "start": 928132, "stop": 929106, "strand": "+", "frame": 1, "gene": null, "product": "ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_A0A3P8KMA1" ], "nt": "ATGGATTTTAATTTAGATGACTTATTTGGTTCTAATGACAATAAAAGTAAAAAATCAAGTGATCCATTAAAAATTTATGGAAGAAACTTAACTGATTTAGCTGCTCGGAATGAATTAGATCCTGTTATTAATCGTGATGATGAAATCAGAAGAATGATTCGCATTTTGAGTAGGAAAACAAAAAACAACCCAGTATTAGTCGGCGAACCAGGTGTTGGTAAAACAGCTATTGTTGAAGGATTGGCTAGAAAAATAGTTGAGGGTCAAGTACCAGAAAACTTAAAAGGTAAAGATATTTTTGAACTTGACTTAGCTGCTTTAATAGCTGGCGCTTCATATCAAGGACAATTTGAAAAGAGATTAAAAGACGTACTTAAAAGAATTGAAGAATCTAATGGTGACATTATAGTTTTCATTGATGAAATTCACATGCTTATTGGCACAGGTCGAAATGCTGAAGGTGGTATGGATGCTGCTAACATCATTAAACCTTTAATGGCCAGAGGTAAAATGCATTTAATTGGTGCTACAACTTTTGATGAATATAGAAAATATATAGAAAAAGATGCGGCTCTTGAAAGAAGAATGCAAAGAGTGGATGTATTAGAGCCTTCAATTGAAGATACTATTACAATTCTTCGTGGTATCAAAGACCGTTTTGAAAATTACCACAATGTAAAAATTCAAGATGATGCTTTAGTAGCTGCTGCTCGTCTTTCTTCAAGATATATTTCTGATAGATTTTTACCAGATAAAGCTATTGACTTAGTTGATGAAGCTGCTGCAACTATTAAAACAGAAATAAACTTTCAACCTGAAGAATTAGAAAAAGCTAAACAAAAAATGGCTAGATTGAAAATGGAAAAAATAGCCATTTCTAGTGAAGACAAGAAAAAACATGCAGTTAGACTTAAAGAAATTGATGCCGAAATAAAAGATATTGAAAAAACAATAAAACAATTAGAAGATAAATGA", "aa": "MDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDK", "aa_hexdigest": "bee78580c5c23544b9149be44aecdab8", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002AC75C79", "query_cov": 0.8858024691358025, "subject_cov": 0.9896551724137931, "identity": 0.634, "score": 347.0, "evalue": 1.51e-116, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [], "truncated": "both", "pseudogene": { "start": 928060, "stop": 929409, "observations": { "mutation": [ 928099, 928108, 929104, 929350 ], "stop": [ 928099, 928108, 929104, 929350 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KMA1", "query_cov": 1.0, "subject_cov": 0.43724696356275305, "identity": 1.0, "score": 611.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 324, "reference_start": 25, "reference_end": 348, "reference_sequence": "MIFGTLTLKVLKLWYNWFSIGGEIMDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDKWNFEKQRLEELSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSKWTKIPISKLLESDKEKLLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA" }, "paralog": false, "identity": 0.9888641425389755, "score": 847.0, "evalue": 2.65e-309, "description": "Internal stop codon at 928099, 928108, 929104, 929350. Nonsense mutation around 928099, 928108, 929104, 929350.", "psc": { "uniref90_id": "UniRef90_A0A3P8KMA1", "product": "ATPase", "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KMA1", "UniRef:UniRef50_I5D648" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "IGIGPGHMMC_1632", "locus": "GCA000148625_01629" }, { "type": "cds", "sequence": "contig_1", "start": 929134, "stop": 929352, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTAATTTAAAAAGTAAATTAGATGACGCAAGACATAGATTAAATATTTACCAAAAAGAAACAGATTATGAAAAAGCTTCAAAAATTTTGTATGATGAAATTCCAAGCATTAAGAAACAAATCGAAGAAACAGAAAAAGAAATAGCAGAATCAGGTAACACAATTGTTAAAGATAGTGTTACAAGTGAAGAAATTGCTAACATTGTTTCGAAATGA", "aa": "MSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSK", "aa_hexdigest": "44b4d03dc0ad878358a320492855b9e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8257.164299999997, "isoelectric_point": 5.130139732360839 }, "id": "IGIGPGHMMC_1633", "locus": "GCA000148625_01630" }, { "type": "cds", "sequence": "contig_1", "start": 929398, "stop": 930285, "strand": "+", "frame": 1, "gene": null, "product": "ATPase, AAA family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "nt": "TTGTTAAATCTTGAAAACGATTTAAAGAAAAGAATAAAAGGGCAAGATGCTGCCATTAGATTAGTTGCTCAAGCTGTATTAAGAGCTAAAGCAAATATTAATGATCCTAATAGGCCTTTAGCAAGTTTCTTATTTACAGGACCTACTGGTGTTGGTAAAACAGAATTAGCAAGAGCTCTTGCATATTCTCTTTTTGATAGTGAAAAACAAATGATTCGTTTAGACATGTCTGAATACATGGAAAAACATAGTGTTTCTAAAATCATTGGTGCTCCTCCAGGATATGTTGGATATGACAGCGGTGGATCATTAACTGAAAAAATTAGAAAGAATCCTTACACAATTCTTTTATTCGATGAAATCGAAAAAGCTGACCGCGATGTTCTTAACATTCTTTTACAAATGATGGATAATGGGGCTATTACAGACTCAAAAGGCCGTGTAATTAACTGTCGTAATTTAATTATAATTATGACAAGTAATTTAGGCTCACAAGAAATACTTAAACATTTAGATCAAATGCCAAGTATTAAAGCTGAATTGCTTAAATTCTTGAGTCCTGAATTTGTAAACAGAATTGATGAAATTGTCAAATTTAATCCATTAACTAAAGATGTAATTAATGAAATTGTTGAACTTGAACTTCAAAAATTAGCTACTAGAATGAAAGATGTTAAAGATGTAAAAATATCATTCACAAGCAAAACTGTTGACTTTGTAGGTTCTTCTTCATATGATGAAAACTTTGGGGCAAGGCCTATTAAAAGATTTATCCAAAATAAAATTGAAAGCTTATTAGCTTACAAGATTATTGATGGCACCATAAGAAGCGGAAAAGCCTATAAAATAGACGTTTTCGCCGGAAACTTTGTTGTTAAAGAAGCCTAA", "aa": "MLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA", "aa_hexdigest": "a3df4c8b16e27c37e6fa183f8abd6563", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F4C5962", "query_cov": 1.006779661016949, "subject_cov": 0.8114754098360656, "identity": 0.529, "score": 324.0, "evalue": 4.09e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_F9PSX5" }, "pscc": { "uniref50_id": "UniRef50_F9PSX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "product": "ATPase, AAA family" }, "genes": [], "id": "IGIGPGHMMC_1634", "locus": "GCA000148625_01631" }, { "type": "cds", "sequence": "contig_1", "start": 930401, "stop": 930628, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTATCCATTTTTTCAATTAATGTTTTATTAACTGAAGTACGGCGAATAACATCTTCTTTTGAAGTAAAATCTTTAGCATCTCTAGCTTCTACTAAAGATACTGCAGCTGCATCACCTAATCCATCAATTGATTTAAAAGGAGGAATTAAACAACCGTGTTTATTGTCAATAATTCATTCATGTGCTGCCGATTTTTTCAAGTCAATATTAGCAATATAG", "aa": "MLSLSIFSINVLLTEVRRITSSFEVKSLASLASTKDTAAASPNPSIDLKGGIKQPCLLSIIHSCAADFFKSILAI", "aa_hexdigest": "9d82021fc778bcb9c662e20b866a2073", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7952.268399999997, "isoelectric_point": 8.768876838684083 }, "id": "IGIGPGHMMC_1635", "locus": "GCA000148625_01632" }, { "type": "cds", "sequence": "contig_1", "start": 930583, "stop": 930768, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATATTAAAGGTGGACATAAAGTTACAGACAGATTGAATCAATTGAATAACATTCCAAAGAATGAAAGAAAAGTTAAAGATGATGACTTAATTCCAGTATTTGAAATAGCTCAAGAACTTTATGCTCGTGGTTTCTATATTGCTAATATTGACTTGAAAAAATCGGCAGCACATGAATGA", "aa": "MIDIKGGHKVTDRLNQLNNIPKNERKVKDDDLIPVFEIAQELYARGFYIANIDLKKSAAHE", "aa_hexdigest": "95c467d4d1a1aa75eca96e0ba2a7235b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7007.9381, "isoelectric_point": 6.723052406311036 }, "id": "IGIGPGHMMC_1636", "locus": "GCA000148625_01633" }, { "type": "cds", "sequence": "contig_1", "start": 930931, "stop": 931383, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGCTTAAAAAATATACCAATATTAATATTGATGACATTCCAAAAAATGATCCTAAAGTTTTAAGTTTATTTGCAAGTACTAAAGCTATGAATATTAAACCTGAACAAATTAATAATGAAACAACAGGGGCATTAGGTTTGCCTGAATTCGGAACAAGTTTTGTTCGTCAAATGTTAAAAGAAGCTAAACCAAAATCATTTGCTAACCTTATTTCTTTAGCTGGACTTTCTCATGGTGAAAATGTTTGGTTGAACAATGCTCAAGAATTAGTAAGCAAAAAGAATTTTGAAATAACTGATGTTATTTGTTGTCGTGACGACATTATGCAATTTCTTTTAAAGAAAAAAGTTGAACCTTTATTTGCTTTCAATATTATGGAAAAAGTTCGTAAAGGTAAAGGTTTAACTGATGATGAAGTTAAGAGATTAAAAGAATATAAAGTGGCTGACTGA", "aa": "MLKKYTNINIDDIPKNDPKVLSLFASTKAMNIKPEQINNETTGALGLPEFGTSFVRQMLKEAKPKSFANLISLAGLSHGENVWLNNAQELVSKKNFEITDVICCRDDIMQFLLKKKVEPLFAFNIMEKVRKGKGLTDDEVKRLKEYKVAD", "aa_hexdigest": "fbb2e4c24cab980a61775e214946f624", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 149, "aa_cov": 0.88, "hmm_cov": 0.8993288590604027, "evalue": 2.4749192436908517e-23, "score": 78.26101684570312, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 17006.64089999999, "isoelectric_point": 9.001349830627444 }, "id": "IGIGPGHMMC_1637", "locus": "GCA000148625_01634" }, { "type": "cds", "sequence": "contig_1", "start": 931471, "stop": 931932, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07", "PFAM:PF07733.18" ], "nt": "TTGTCAAAAGATGAAAAAATAACAAGTGGATATGACTTACCTAACAAAAATTGCCCTAAATGTAATATTTTATTAGAAGCTGATGGACAAGGAATTCCTTTTGAAACCTTCTTAGGTTTTAAAGCTGATAAAGTTCCTGATATTGACCTTAACTTCTCAGGCGATATTCAAGGTGATATTCACAATGAAGTTAAACGTATTTTTGGTAATACTCACACTTTAAAAGCTGGTACCATATCAACAGTTGCTCTTAAAACTTCATATGGACATGTTAAAGCATTTACAGAAGAAGCAATGCGTAATTATTCAGAACCATTTATTGACTTTTTAGCCCATAAAATAGAAAAATTGAAAAGAACTACAGGCCAACACCCAGGTGGTATTCTTATTATTCCAAAAGAATTTGATGTCTATGATTTTACCCCAATAAACTATCCAGCCAATGAACCTGATTCTTCTTGA", "aa": "MSKDEKITSGYDLPNKNCPKCNILLEADGQGIPFETFLGFKADKVPDIDLNFSGDIQGDIHNEVKRIFGNTHTLKAGTISTVALKTSYGHVKAFTEEAMRNYSEPFIDFLAHKIEKLKRTTGQHPGGILIIPKEFDVYDFTPINYPANEPDSS", "aa_hexdigest": "ccdd409a814e3b155175f57066507a88", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002285EE07", "query_cov": 0.9738562091503268, "subject_cov": 0.8662790697674418, "identity": 0.617, "score": 204.0, "evalue": 1.03e-64, "valid": false, "uniref50_id": "UniRef50_UPI002285EE07" }, "pscc": { "uniref50_id": "UniRef50_UPI002285EE07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 62, "aa_cov": 0.40522875816993464, "hmm_cov": 1.0, "evalue": 1.7502630270146172e-23, "score": 79.51678466796875, "start": 30, "stop": 91 } ], "seq_stats": { "molecular_weight": 17051.052199999984, "isoelectric_point": 5.4928293228149405 }, "id": "IGIGPGHMMC_1638", "locus": "GCA000148625_01635" }, { "type": "cds", "sequence": "contig_1", "start": 932089, "stop": 932535, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGCTACAGCTGATGTTAAGTATCTAAATTTATATGACAAAGTAGCTTATGAGGTTTTAGTTTATGCTAAAGGAATTAAAAACTCTCGTCATTACTTATTCAACTATGAACAAGCTAAAAACGGAAATATTCCAATTCCAAATCAAGACTATTTAACCACTGAAGAAATGCTTGAACAATTTGCCTTTTTAAAAGACAAACAATTAATTGAAGACATAGTTATAAACAACACAAATAAAATAGCAGACATGTGCGACAAAATTCAAGTTATCAAAGATAAACTTTATACACCTAAGTTTGAAGACAGCGGTAAAAAACTACATGATTTAGTTTATGAAACAGCATACAAAAAATATGGTGAAAAATTACCAAAAATAGTTGAAGAAAGAATTGAAGCCGAATTAAATCCTATTATTGATTATGGTTTTAGTGTTATTTATTGA", "aa": "MVATADVKYLNLYDKVAYEVLVYAKGIKNSRHYLFNYEQAKNGNIPIPNQDYLTTEEMLEQFAFLKDKQLIEDIVINNTNKIADMCDKIQVIKDKLYTPKFEDSGKKLHDLVYETAYKKYGEKLPKIVEERIEAELNPIIDYGFSVIY", "aa_hexdigest": "10c447d9ea582f3c74b17df5b288700d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17316.68769999999, "isoelectric_point": 5.271327781677246 }, "id": "IGIGPGHMMC_1639", "locus": "GCA000148625_01636" }, { "type": "cds", "sequence": "contig_1", "start": 932620, "stop": 932976, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGAATTAGGTAAAAAAGGTATTTTAACCTTTAATCAACTTAATTCATATATTAAAGATGATCTTTATAATAAAACCTTTTCATATGAAGTTTCAACAATAGCTAAAAACTATGAAGGTTTAAAAGAACAATATGGTTATTTAACAACAGCTTTAACTAAAAACTTATACAATGGACCTAAAACATTTATGGAAGATTTAGCAGCTCATAGTGAAAATCTTTTATTAGGTTCAGGAACACTAAAAAGTAGATTGCTTGATGTTTATTTTTATTCTTCACATGAAGAATTCATTAAAGAATTAAAACTTTATGACTATATTGAAGTGCCAGCACCTCAAGTGTTTAGCCATTGA", "aa": "MDELGKKGILTFNQLNSYIKDDLYNKTFSYEVSTIAKNYEGLKEQYGYLTTALTKNLYNGPKTFMEDLAAHSENLLLGSGTLKSRLLDVYFYSSHEEFIKELKLYDYIEVPAPQVFSH", "aa_hexdigest": "42cf8305374339e3d414d753f2e741cf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13625.23669999999, "isoelectric_point": 5.308500480651855 }, "id": "IGIGPGHMMC_1640", "locus": "GCA000148625_01637" }, { "type": "cds", "sequence": "contig_1", "start": 932986, "stop": 933705, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGAACACAATGGATGGATTAATTTTTCCTAAAGACATTGTGGCTTTTGCTGAAAAATTGAATCATTCTGCTGTTGCAATTATGGACGTTGATGGTGCTCAAGGTTATCCTGAATTTTATAATGCAGCTAAAAAATCAAAAGTTAAACCTCTTTTTGGAACTGCGTTTTCAGTTATAAACAAAAGTAATAAATCTATTTTAGGAAACATACCTGAAGGCGAAATTAAAGGTCAAGAATATGTTTCATTCGATATTGAAACCACTGGACTTTGTCCTCGTTTTGCCGAAATTATTGAATATGGTTCATTGCCAATTCACTCAAACATGAAGTATGGGAGAAAAACACAATTTTTCATTAAATGTCAAGAACCAATTAAACCTTTTACAGAAAACTTGACAGGTATTACTAATGCAATGATTGAAAAAAGTGGTTTGGAATTAAAAGAAGCTTTGCAAAAAATTTATAATGACTTAAATGGCAAAATTGCTTTAGCACACAATGCTAGATTTGACTTCAACTTCTTAAAAGAAAAATTTAGACAAGCTAAAATAGAATTTCCAAATGTAACAGTAATTGACACACTTATTGTTTCAAGAATTGTTTTCCCTCAAAACAAAAAACATAAACTTGAAGATGTTGCTACTCGTGTTGGTGTTGAATATGATCCTACAGTTGCTCACCGTGGTGATTATGATGCGAAAGTTTTAGCTGAAGTTTGA", "aa": "MNTMDGLIFPKDIVAFAEKLNHSAVAIMDVDGAQGYPEFYNAAKKSKVKPLFGTAFSVINKSNKSILGNIPEGEIKGQEYVSFDIETTGLCPRFAEIIEYGSLPIHSNMKYGRKTQFFIKCQEPIKPFTENLTGITNAMIEKSGLELKEALQKIYNDLNGKIALAHNARFDFNFLKEKFRQAKIEFPNVTVIDTLIVSRIVFPQNKKHKLEDVATRVGVEYDPTVAHRGDYDAKVLAEV", "aa_hexdigest": "1ab0ccf5d4f57d2ea0d78d78c878f89b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 142, "aa_cov": 0.5857740585774058, "hmm_cov": 0.8169014084507042, "evalue": 3.8753411160511375e-06, "score": 22.935955047607422, "start": 83, "stop": 222 }, { "id": "PF00929.31", "name": "RNase_T", "length": 166, "aa_cov": 0.6652719665271967, "hmm_cov": 0.9819277108433735, "evalue": 1.611383493678028e-27, "score": 93.06481170654297, "start": 81, "stop": 239 } ], "seq_stats": { "molecular_weight": 26840.58710000003, "isoelectric_point": 7.715233802795409 }, "id": "IGIGPGHMMC_1641", "locus": "GCA000148625_01638" }, { "type": "cds", "sequence": "contig_1", "start": 933862, "stop": 934014, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATTCTCAAGACTATGCTTTAAGTAAAAATGGTAAACACATTTACACATTTTTTCTAACCAACTATAAAGATGCTGTAGAAGTAAAAACAATAAGAACAACATTATTAGAACCTGAACAAATAAATGAGCTTGAAGACAACAAATGA", "aa": "MIYSQDYALSKNGKHIYTFFLTNYKDAVEVKTIRTTLLEPEQINELEDNK", "aa_hexdigest": "51ce6b88c245a52c9ac8810f9f25e88d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5926.615999999998, "isoelectric_point": 5.12309169769287 }, "id": "IGIGPGHMMC_1642", "locus": "GCA000148625_01639" }, { "type": "cds", "sequence": "contig_1", "start": 934264, "stop": 934743, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATAGTAATAATATGCCTAAAGAAAATAGAAAAATAGAAAATAAAAATTTCATTACATTTTGTAAAGATATTAATGTAAAAGTGACACCTGGATTGGATAAAGTTGAATTAGACGATGTCGAATATGACAGTGTTAAAGACCAATTAAATGTGTCATTTAAATTTACAAATTCAATCAGTGCAAAGGAATTTTTTTCGTTTCTTTCTAGTTTAAAAAATAATAAAGTTTATAACATCAAAGGTAAAATTACTTTTGATATTTCACAATATAAAAAAGAAAATTTAATAGATTTTATTAACGAATTAACCTATGAAAAAGCCAAATTCATCAAATTGAGAAATTTGCCAATTGTTAAAAAACTTAAATTTAATGATCAATTTGTAGCAACAATTACAACTATTTCAGCTAAAGAATATGAAGAGTTTAACCCCGTTTTTAAAAACCTTCAAAAAACTTTAAATAAATACGGTTGA", "aa": "MYNSNNMPKENRKIENKNFITFCKDINVKVTPGLDKVELDDVEYDSVKDQLNVSFKFTNSISAKEFFSFLSSLKNNKVYNIKGKITFDISQYKKENLIDFINELTYEKAKFIKLRNLPIVKKLKFNDQFVATITTISAKEYEEFNPVFKNLQKTLNKYG", "aa_hexdigest": "9e768587300baf237b9550e6608e9f59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18684.2659, "isoelectric_point": 9.30112781524658 }, "id": "IGIGPGHMMC_1643", "locus": "GCA000148625_01640" }, { "type": "cds", "sequence": "contig_1", "start": 935224, "stop": 935571, "strand": "-", "frame": 2, "gene": null, "product": "MAG0920 family protein", "db_xrefs": [ "RefSeq:WP_245525948.1", "SO:0001217", "UniParc:UPI0010051079", "UniRef:UniRef100_UPI0010051079", "UniRef:UniRef50_UPI00217F505D", "UniRef:UniRef90_UPI00217F505D" ], "nt": "TTGAAATTCAACAATAATTTAAATGACGAAAATCTTTATGAAAAGAAGATTAATTCAGAAACTAACAAAACACAATTTGATTTTTCATTAGGCCAAAAATATCACTATAACTCCCTTCCAAATGAAAAGTCGTTAGTTTCATTCTTTTTCAGATCAGTCGATTTTTATATTAAATATCCAGAAAATTTTGAAAACTTATCTCCTAAAAAGCAAAGAAAATTAGTATATGCAAATTCAATCTTCGATTTTGAAAATACTAAGCCTGATAAATTAACATATTTTGATATTAATATGTTTAAAGACACATTTATTAAATATCAAATTATCCAGAATAATAAAAATGATTAG", "aa": "MKFNNNLNDENLYEKKINSETNKTQFDFSLGQKYHYNSLPNEKSLVSFFFRSVDFYIKYPENFENLSPKKQRKLVYANSIFDFENTKPDKLTYFDINMFKDTFIKYQIIQNNKND", "aa_hexdigest": "08012eba18b2f42d4acc758c829d58d6", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0010051079", "ncbi_nrp_id": "WP_245525948.1", "uniref100_id": "UniRef100_UPI0010051079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051079", "RefSeq:WP_245525948.1", "UniRef:UniRef100_UPI0010051079" ] }, "ips": { "uniref100_id": "UniRef100_UPI0010051079", "uniref90_id": "UniRef90_UPI00217F505D" }, "psc": { "uniref90_id": "UniRef90_UPI00217F505D", "product": "MAG0920 family protein", "uniref50_id": "UniRef50_UPI00217F505D" }, "pscc": { "uniref50_id": "UniRef50_UPI00217F505D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F505D" ], "product": "MAG0920 family protein" }, "genes": [], "id": "IGIGPGHMMC_1644", "locus": "GCA000148625_01641" }, { "type": "cds", "sequence": "contig_1", "start": 935982, "stop": 936272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5", "UniRef:UniRef90_C4XFQ5" ], "nt": "ATGCTTCCAGATAGTTCTTTAATCGAAAAATCAATTGACATAAACACAGATGTTGAACAATTGTATCCATCTAAAAATAAATGTTATTTTAAAAGACAAGGTTTTAATGCACTTTATTTTTCAACTTTTAATACTCAAAAATTCAAGTCATACTCATTCGAAAAGCAAAAACTTTTTATTTATATGACTATGGTTCTCAATTACGATGATACAGCATTTGATAATAAATTTGAGCCGCTAAATATTAATATGTTCAAAAATGAAGCAAAAGCTTATAAAATTATTGAATAA", "aa": "MLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE", "aa_hexdigest": "9461ed71d51c23faf2888eab3867f672", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 935982, "stop": 936572, "observations": { "mutation": [ 936290, 936311, 936335 ], "stop": [ 936290, 936311, 936335 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ5", "query_cov": 1.0, "subject_cov": 0.41739130434782606, "identity": 1.0, "score": 194.0, "evalue": 4.1e-61, "gene_start": 1, "gene_end": 96, "reference_start": 135, "reference_end": 230, "reference_sequence": "MSNFLIFSIIFFSFWIAFINLTPFIILFPKIRWYIYAFGSVNKYCMKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPLWYIVVCLLWYIKQKKWRKFNNMLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE" }, "paralog": false, "identity": 0.9846938775510204, "score": 383.0, "evalue": 5.08e-138, "description": "Internal stop codon at 936290, 936311, 936335. Nonsense mutation around 936290, 936311, 936335.", "psc": { "uniref90_id": "UniRef90_C4XFQ5", "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ5", "UniRef:UniRef50_C4XFQ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11489.03199999999, "isoelectric_point": 7.744960594177246 }, "id": "IGIGPGHMMC_1645", "locus": "GCA000148625_01642" }, { "type": "cds", "sequence": "contig_1", "start": 936333, "stop": 936539, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAATTAAGAGTTCAAAATTCATTAGTAAATCATTATTGTCTGATGTCTAAAATTCCATTAATTTATGCTTTACTTGCAATCGCATTAATGCTAGAAAGCTTAGTTTTAATGGTTTTTCTACAAGTATATGAAAAGGAATCATTTTTTACAATGTTTGTAACACTTGGCATAATATTTTATGTACCATTGCCCTTGTGA", "aa": "MKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPL", "aa_hexdigest": "73034ecf5c94e4b169ab768fbfd9b822", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.764499999995, "isoelectric_point": 9.301514625549316 }, "id": "IGIGPGHMMC_1646", "locus": "GCA000148625_01643" }, { "type": "cds", "sequence": "contig_1", "start": 936601, "stop": 936876, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGCGTAAGATTAATAAAAGCAATTCAAAAACTAAAAAAGATAATTGAAAAAATTAAAAAATTACTCATATGTGATTATCCTAAATTATCATATAAAGAATTATTAGCTTTTTTACGCAAATATGTTCCTTTTTTAGGGTCATTAACTCTTTGTAAACTTCACAAACTTAATTCAAAATCAGAAAGCTCTAGCATTTCATTAATAGTTTTTATTTCGTTGGTTTCTAAATTTTTAAAAATAAGGTTTTTTCCACCAGTACCTCCCATATAA", "aa": "MNGVRLIKAIQKLKKIIEKIKKLLICDYPKLSYKELLAFLRKYVPFLGSLTLCKLHKLNSKSESSSISLIVFISLVSKFLKIRFFPPVPPI", "aa_hexdigest": "5f1d287fa27a157290594ec3daa0662e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10476.840899999988, "isoelectric_point": 10.203556251525882 }, "id": "IGIGPGHMMC_1647", "locus": "GCA000148625_01644" }, { "type": "cds", "sequence": "contig_1", "start": 936912, "stop": 937166, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCAAGTATTCCTATGGCATTAAATCGTGTTGCTAGAGTATATGAAATGCTTAAATTATCACAAGCTGCGCCATCAATAGCAACTATGGCTACTGGTGTTGTTGGTTCAGCATTACAAACTCTTGGATCATTTTATGATGTTTACACCGCTTCAAAAGAATTAGATTATTTTAATGAATGTGCTAAAAAGGCATCAGATTTAATTAGTGATTTTTCAAAGAAAATAAAAGATATTAAAAAAATAGGATGA", "aa": "MVSSIPMALNRVARVYEMLKLSQAAPSIATMATGVVGSALQTLGSFYDVYTASKELDYFNECAKKASDLISDFSKKIKDIKKIG", "aa_hexdigest": "739759822f8d92f287065c50fef326ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9127.559099999993, "isoelectric_point": 9.09850368499756 }, "id": "IGIGPGHMMC_1648", "locus": "GCA000148625_01645" }, { "type": "cds", "sequence": "contig_1", "start": 937542, "stop": 937991, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTATCTACTTTACTAAATACAATTAATGTAACTAACATAACACCATCAGTCATTGTTGCATCAAATGAAAATTTAAATTCAGAGCAACAAAAAATTTTGGAATTATTTTCTCGATTTACAAACGATGAAATTGAAATTTTTAACTATATCTCAAATGATATTATCAATAACAAAAATTCAACTAAAAAAGTTATAAATTCCATTGATGAAATAAAATTAGATAAAATTTATGATTTGTTTATATCTAAAATTAGCCAATCTAGTATAAAAAATAAAGATGAATTATTAAATAAACTAAGAGACAAAAGAGAAATTTTTAAAACTGAATTAATTAAAAAAATAAAAGAACAATCTTTAACTTTTAAAAAAAGCGAAAAAAAATCAAAAATAGAAATCAATTCAATGATAAAAAGAGTCAATTCGTCTTTTGAAAATAAAGATTGA", "aa": "MKLSTLLNTINVTNITPSVIVASNENLNSEQQKILELFSRFTNDEIEIFNYISNDIINNKNSTKKVINSIDEIKLDKIYDLFISKISQSSIKNKDELLNKLRDKREIFKTELIKKIKEQSLTFKKSEKKSKIEINSMIKRVNSSFENKD", "aa_hexdigest": "f4afcd0e1f87f7841e4c727d1bb7c2e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17375.80749999999, "isoelectric_point": 9.397314643859861 }, "id": "IGIGPGHMMC_1649", "locus": "GCA000148625_01646" }, { "type": "cds", "sequence": "contig_1", "start": 938447, "stop": 938788, "strand": "-", "frame": 1, "gene": "exiS", "product": "ExiS", "db_xrefs": [ "RefSeq:WP_013355043.1", "SO:0001217", "UniParc:UPI0001E3307C", "UniRef:UniRef100_A0AB32XDB6", "UniRef:UniRef50_A0AB32XDB6", "UniRef:UniRef90_A0AB32XDB6" ], "nt": "ATGTGTAAAGTTTCAGATTACAATGTAGCAAAACAGTTAATATTGTTTTTTATTTTACAATTATTTTCAATGTTAAAAGTTCCTACCTATAAAGATTTAAATATAAATATTAAAAAGATTTTGTGGACTAAAAATTATAAAAGCTTTACAGAGTTCCAAAAGGCATATAGTGGCGTTTTTTGTGCTAAATTAGGTCATTATCTAAGTAAGCATAAAGCCAACACCAAAACAAATTTTTTAAATATGTATCAAATTATTTTTTATCAAGGTTTTATTAATTACAACAGAGAAAAGGAATTATTAGATTTATTGACTTATAAAGAAAAGTCTCAGACATATTAA", "aa": "MCKVSDYNVAKQLILFFILQLFSMLKVPTYKDLNINIKKILWTKNYKSFTEFQKAYSGVFCAKLGHYLSKHKANTKTNFLNMYQIIFYQGFINYNREKELLDLLTYKEKSQTY", "aa_hexdigest": "ca41be5796e33309c75e728d0372d953", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3307C", "ncbi_nrp_id": "WP_013355043.1", "uniref100_id": "UniRef100_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307C", "RefSeq:WP_013355043.1", "UniRef:UniRef100_A0AB32XDB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDB6", "uniref90_id": "UniRef90_A0AB32XDB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDB6", "product": "ExiS", "uniref50_id": "UniRef50_A0AB32XDB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDB6" ], "product": "ExiS" }, "genes": [ "exiS" ], "id": "IGIGPGHMMC_1650", "locus": "GCA000148625_01647" }, { "type": "cds", "sequence": "contig_1", "start": 939506, "stop": 939826, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAATAATATCATCAAAATTAGTTTTTTTTCTTAAAAAATCTTCTAACTAA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSIISSKLVFFLKKSSN", "aa_hexdigest": "920815c94b6ebd0c1000b79640b9735d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12364.57399999998, "isoelectric_point": 9.96186428070068 }, "id": "IGIGPGHMMC_1651", "locus": "GCA000148625_01648" }, { "type": "cds", "sequence": "contig_1", "start": 940082, "stop": 940354, "strand": "-", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_259637433.1", "SO:0001217", "UniParc:UPI000FF5E58A", "UniRef:UniRef100_C4XFQ1", "UniRef:UniRef50_C4XFQ1", "UniRef:UniRef90_C4XFQ1" ], "nt": "ATGACAAGTACTATTTCGCAATTTGCTTCACAATACCAAATGAGTAATTTACAAAAGTATCGTAGTGTAGAAACAATAATTAATATGGAAAAAGCCATATTCTTGAAAAATCGTCAAGAAGATTTTATAGAAAAATATTACAAGAAGAATGAAAATTCAAGATCTAATAATTACTTTTCAGACAAATTCAAAAGAGTATACAATTGCTTAAGCGGTGTAAGTCAACTGATCATTAAAAATGAGTTTATGAAACAATCTGAAAACAAGTTATGA", "aa": "MTSTISQFASQYQMSNLQKYRSVETIINMEKAIFLKNRQEDFIEKYYKKNENSRSNNYFSDKFKRVYNCLSGVSQLIIKNEFMKQSENKL", "aa_hexdigest": "de9335703414c11ffa78d0d92e3af720", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000FF5E58A", "ncbi_nrp_id": "WP_259637433.1", "uniref100_id": "UniRef100_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FF5E58A", "RefSeq:WP_259637433.1", "UniRef:UniRef100_C4XFQ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFQ1", "uniref90_id": "UniRef90_C4XFQ1" }, "psc": { "uniref90_id": "UniRef90_C4XFQ1", "product": "Transposase", "uniref50_id": "UniRef50_C4XFQ1" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ1" ], "product": "Transposase" }, "genes": [], "id": "IGIGPGHMMC_1652", "locus": "GCA000148625_01649" }, { "type": "cds", "sequence": "contig_1", "start": 940396, "stop": 941301, "strand": "-", "frame": 2, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_Q98R27", "UniRef:UniRef90_C4XFQ0" ], "nt": "ATGATTTCAACTTATTCAAAATTTTTAAAAAACTTTTTAGCAATACCAACAATTATTGGTAAGAAAACGCCAAAAGAAAAATTTGCTGGCGCATGTTCAACTTATACAGTTGAAGCAATGATGAAGGATGGTCGTGCTTTACAAGCAGGAACAAGCCATTATTTAGCTCAAAACTTTTCAAAACAATTTGAAATTCAATTCAAAACTGAAAACAATGAATTAGATTATGCTTACCAAACTTCATGGGGTGTTTCAACCCGTCTGCTTGGAGCTATTATAATGACTCATGGAGACAATAGAGGAATTATTATTCCACCTCGAGTAGCTCCAATTCAAATAGATGTTTTAGAGCTTTTTGCAAATAAAAATAAAGAAGTTCATACATACTGTGATAATCTTTACAAAGACCTAAGTAGAAAATTTAGAGTAAGACTTGATTCAAGTGACAAAGGTCCAGGATTTAAAGCCTCAAATTCTGAAATTCAAGGTGTTCCTCTTAGAATTGAAGTTGGACCTAAAGACATTGAAAATAAAAGTGTGACAATTGTTAGAAGAGACACCCTTGAAAAGATTAATATAAAACAATCAGAAGTTAAGGATCAAGTTGAAACTCTTTTAAATCAAATCCACGATAACTTGTACTTACAAGCTGAAAAAAGACTAAATGAAAATACAGTCTTTGTAGATAACTACGAAGATTTCAAAAAACAAATTAAAAATCATAAATTTGTTATAGCCCCATTTTGTTGTTTAGATGTGGCTGAAGAAATAATCAAAAATGAAACTGGCGCTTCAACTCGTTGCTTACCTAAAAAGTTTGATAAACCAAATCAAAAACACAAATGTATTTTTGAAAAATGTTGTAAAGAAACCAATAGATATGTAATCTTTGCAAGAGCTTACTAG", "aa": "MISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY", "aa_hexdigest": "2e3c870226400edc3a969c193508d9d3", "start_type": "ATG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "3-prime", "pseudogene": { "start": 940396, "stop": 941601, "observations": { "mutation": [ 941361, 941406, 941424 ], "stop": [ 941361, 941406, 941424 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ0", "query_cov": 1.0, "subject_cov": 0.6244813278008299, "identity": 1.0, "score": 604.0, "evalue": 1.44e-215, "gene_start": 1, "gene_end": 301, "reference_start": 182, "reference_end": 482, "reference_sequence": "MIKIMKELEKITPLEQDFAKWYTDVVKQGNLIAYGPVKGTIVFKPNSYGIWEQIQKNLNECFREKGVQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQWANVVRWEKTTNPFLRSREFLWQEGHTCHSNPLEARKFTKGMISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY" }, "paralog": false, "identity": 0.9925187032418953, "score": 801.0, "evalue": 7.33e-296, "description": "Internal stop codon at 941361, 941406, 941424. Nonsense mutation around 941361, 941406, 941424.", "psc": { "uniref90_id": "UniRef90_C4XFQ0", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q98R27", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ0", "EC:6.1.1.15", "UniRef:UniRef50_Q98R27", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R27" ], "product": "Proline--tRNA ligase" } }, "id": "IGIGPGHMMC_1653", "locus": "GCA000148625_01650" }, { "type": "cds", "sequence": "contig_1", "start": 941422, "stop": 941646, "strand": "-", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "GTGCAAAATGTATATTTGCCACTTTTAATACCTGAAAAATTGTTTAACAAAGAAAAAGATCACATAGCAGGATTTAATCCAGAATTAGCAACAATTACTAAAGTTGGCGACAAGGATTTAGATGAAAAAGTATTTATTAGACCAACATCAGAAGTTTTATTTGCTGATTTATTTAAAAACTCAATTGAGTCTTACAAAGACTTACCTATGATTTATAACCAATGA", "aa": "MQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQ", "aa_hexdigest": "22489394e1143e09c5d48d3cbf0b2e0b", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 0.891566265060241, "identity": 0.527, "score": 87.8, "evalue": 2.74e-21, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1654", "locus": "GCA000148625_01651" }, { "type": "cds", "sequence": "contig_1", "start": 941964, "stop": 942116, "strand": "-", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013355046.1", "SO:0001217", "UniParc:UPI0001E3307F", "UniRef:UniRef100_C4XFP9", "UniRef:UniRef50_P35871", "UniRef:UniRef90_C4XFP9" ], "nt": "ATGCCAAGAGACGGTTTTACATTAGTTTGCGAATCATGTAAAATGGAAAACTACATTAGTAAAAAGAACAAGAAAAATACACCAGAAAAAGTTGAATTAAGCAAATATTGTTCAAAATGTAGAAAACACCAAAATCACAAAGAAAAGAAATAA", "aa": "MPRDGFTLVCESCKMENYISKKNKKNTPEKVELSKYCSKCRKHQNHKEKK", "aa_hexdigest": "4965179e4d6cbe3993b3906007accba1", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3307F", "ncbi_nrp_id": "WP_013355046.1", "uniref100_id": "UniRef100_C4XFP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307F", "RefSeq:WP_013355046.1", "UniRef:UniRef100_C4XFP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFP9", "uniref90_id": "UniRef90_C4XFP9" }, "psc": { "uniref90_id": "UniRef90_C4XFP9", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "IGIGPGHMMC_1655", "locus": "GCA000148625_01652" }, { "type": "cds", "sequence": "contig_1", "start": 942493, "stop": 942672, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGACAATAATGAATATTTGACTGATGGAGAGTTAGCTTTATCAAATAATGAACCAATACGAAGTCAATGTCGTAGTCCATATTCATATTTTACTAAACCAAAAAGAAGAATAGTTTGCGAAGGATATGCAAGAACATTTTCAAGATTTCTAACATTAATAAATATTCCTGTGTGA", "aa": "MIDNNEYLTDGELALSNNEPIRSQCRSPYSYFTKPKRRIVCEGYARTFSRFLTLINIPV", "aa_hexdigest": "e4f011fdaf9d1c7e48010e50eee049db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6902.802399999999, "isoelectric_point": 8.736062431335448 }, "id": "IGIGPGHMMC_1656", "locus": "GCA000148625_01653" }, { "type": "cds", "sequence": "contig_1", "start": 942910, "stop": 943410, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTAAAGAAAGCTTTAAAACTTAAAAAAGAATTATTAGAATTAAATGAAAAAATTTTTAATGCTTTTGGATTACATGAGGGAAGATTTGAAAGTTATTTAAATGCTTACAAAAATTTCGTTAATATAGGTTTTGTACAATCAAATACAGGATTAACTGCTACAATAAATGGGAATCCTGAACCTACAATAAAATATATTTTAGAGAATTATATATCTCAAGGAAGTCTTCTTAATCTTAATTATAAATTTTTTAACGACATTTTAAGCAATATAGAAAAATCTGTAAAGTACTATAAAAAAGACTTAGACTTATCATTTGACATTAAAGATGCATATGAAGTTGATTTAAACAATTTGGACTTTCTAAAAAGTGCTAAAGCTGTTGATACTCTCACAAAAGAACAAGTTTTAAAAATTAGAAAAGACTTGCTATTTAAAGGAAAAATAACTTCTGATATTGCCGTAACTAATCATAATTTAGGAGATTATTTCTGA", "aa": "MLVKKALKLKKELLELNEKIFNAFGLHEGRFESYLNAYKNFVNIGFVQSNTGLTATINGNPEPTIKYILENYISQGSLLNLNYKFFNDILSNIEKSVKYYKKDLDLSFDIKDAYEVDLNNLDFLKSAKAVDTLTKEQVLKIRKDLLFKGKITSDIAVTNHNLGDYF", "aa_hexdigest": "8a39ac61662c67e5222f7cc6ab116429", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19074.695900000002, "isoelectric_point": 8.552198600769042 }, "id": "IGIGPGHMMC_1657", "locus": "GCA000148625_01654" }, { "type": "cds", "sequence": "contig_1", "start": 943550, "stop": 943768, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGATGGTTTATTATCTCCAGGTTTTGGGGCTGGGCCTGGACCTGGTTCTGGTGTTACAGGTGTAACTGTCGTACTTGGTGTTGAAGGTATATTGCCAGTAACATCATCTCAAAAACATGATGTTGTTATAGTTGGTAGACTAACAATAGCAGTTAGAGTACCTAGTCAATTAAATATTTTATTTTTTTTCATATTTATCTCTTTCTACAATTAA", "aa": "MLDGLLSPGFGAGPGPGSGVTGVTVVLGVEGILPVTSSQKHDVVIVGRLTIAVRVPSQLNILFFFIFISFYN", "aa_hexdigest": "7b7adee789e7d8425142ec843d51200e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7473.685599999998, "isoelectric_point": 6.509280967712402 }, "id": "IGIGPGHMMC_1658", "locus": "GCA000148625_01655" }, { "type": "cds", "sequence": "contig_1", "start": 944363, "stop": 944857, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGAAACTTTATTGATAGAAACAAAAAGGATCTTGAATTAGTTTTTGATTTAAAAGATGCTAAACAAATTAATATTAATAATTTAAAAATGTATACAGAAAATATTAACTCAGTTTTAAACAAACAATTGAATGTTGAACATTTTAGAAAATCATTAGTTAAAAATGGTGTTAATGTTGCTCCTCTTATCAAATATTATTATGGCACGGATAAATATAACAGCTTAAGAAATTTAGTTTATGATCTAACGGGCGGTTCAAACTATTTTAATGATTTTTGTTTTAAAGGAAACCACTATAAAAATTCAACATCTGTTACATTTTATCAACCAGAAAATAATGTAATAAACATTAAAATAAATCGTTTAGCTTATAGAACTGAAAATGCACAAGAAGAAATTGATATCAATAAATATTTTGAAAAAATTATTTATTCTATTATTTCAAAACAAATGAATGATGAACAAAAAATTAGAGCTATTCACAATTGA", "aa": "MFRNFIDRNKKDLELVFDLKDAKQININNLKMYTENINSVLNKQLNVEHFRKSLVKNGVNVAPLIKYYYGTDKYNSLRNLVYDLTGGSNYFNDFCFKGNHYKNSTSVTFYQPENNVINIKINRLAYRTENAQEEIDINKYFEKIIYSIISKQMNDEQKIRAIHN", "aa_hexdigest": "54a25e6fd275c82fd7a801c307af3c49", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19498.898500000003, "isoelectric_point": 9.24742565155029 }, "id": "IGIGPGHMMC_1659", "locus": "GCA000148625_01656" }, { "type": "cds", "sequence": "contig_1", "start": 944942, "stop": 945109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAACAATGATGTTTATGATAAATTTGGTTTGCATGAAAACCGTTATAAAAAATATTTAGTTGCTTACAAAAATTTTATAAATTTATATATTGATAATGAAAATAGCAGAGCTTATATTTTTGATCTTTTAGCTCCAAAACATAATAATTTTTGTTCCTGA", "aa": "MQLNNDVYDKFGLHENRYKKYLVAYKNFINLYIDNENSRAYIFDLLAPKHNNFCS", "aa_hexdigest": "48f75f87877629d512ef708cbdd1566c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6679.4655, "isoelectric_point": 7.863096046447754 }, "id": "IGIGPGHMMC_1660", "locus": "GCA000148625_01657" }, { "type": "cds", "sequence": "contig_1", "start": 945766, "stop": 945915, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAATGATTGACTCAGGAGAAACAGATACAAAATTAATTGCTGTTCATGCTGATGATTATCGTTTAGATGGTATTAAAAAATTAGAAGACTTACCTGAACCATTTTTAATGCACATTGAAACATTCTTTAGCAACTATAAAAATTGA", "aa": "MKMIDSGETDTKLIAVHADDYRLDGIKKLEDLPEPFLMHIETFFSNYKN", "aa_hexdigest": "8655a983f95376eb1fa7e1c087a748f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 49, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 7.4407840897036e-15, "score": 51.09521484375, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5731.486999999998, "isoelectric_point": 4.8155927658081055 }, "id": "IGIGPGHMMC_1661", "locus": "GCA000148625_01658" }, { "type": "cds", "sequence": "contig_1", "start": 946000, "stop": 946158, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAGTAATATTATTGAAATGAAAGTTGAAATTCAAAAGAATTCAAACATAAAATACGAATATGATCGTGCTGACGGAAATATTCATGTAGACCGTATTTTGAGAGGAGATTTTGTCTACCCATGTAATTATGGCTTTATTCCTACAGCTTTAGATTGA", "aa": "MSNIIEMKVEIQKNSNIKYEYDRADGNIHVDRILRGDFVYPCNYGFIPTALD", "aa_hexdigest": "09b23b3bc4c5ae2db9338f44658ea512", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 2.165451389834823e-14, "score": 49.5887451171875, "start": 8, "stop": 51 } ], "seq_stats": { "molecular_weight": 6080.8351, "isoelectric_point": 5.1192834854125975 }, "id": "IGIGPGHMMC_1662", "locus": "GCA000148625_01659" }, { "type": "cds", "sequence": "contig_1", "start": 946311, "stop": 946571, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4", "UniRef:UniRef90_C4XFP4" ], "nt": "ATGATTTTAAACTCAGTATTTTATAAAGCAAATAACCCATTTTACGAACAAGGAACACACAAATTAAATGCTCCTTATTTAGCTTTATTTATTATTGGATTATCACTAATAGTAATTGGAATTACATGCTTTTTCTTTTATCCTAAAGCAAAAGACAAGGTTTATTTGTATAAAGAAAAACAAATGGAAGAATATAAAAAAAATAATCCAAAATCTAAAGTAACGAATTATGAAGCAACAGGAATGTATTTACCAGCTTGA", "aa": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPA", "aa_hexdigest": "2af4347964a77378f824706534822d7e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 946215, "stop": 946571, "observations": { "mutation": [ 946313 ], "stop": [ 946313 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP4", "query_cov": 1.0, "subject_cov": 0.7288135593220338, "identity": 1.0, "score": 176.0, "evalue": 1.57e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPAWERIKLFAPLFFGILFVVVGITMIVGKTISTL" }, "paralog": false, "identity": 0.9915254237288136, "score": 236.0, "evalue": 1.24e-81, "description": "Internal stop codon at 946313. Nonsense mutation around 946313.", "psc": { "uniref90_id": "UniRef90_C4XFP4", "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP4", "UniRef:UniRef50_C4XFP4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10015.642899999993, "isoelectric_point": 9.301836967468262 }, "id": "IGIGPGHMMC_1663", "locus": "GCA000148625_01660" }, { "type": "cds", "sequence": "contig_1", "start": 946684, "stop": 946914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGACTAAAGAATTAAAAAAACCTAAAATAATTTTTATCGATTTGGATGGTACAACAATTGATAAAAGTAGCAAATTTAAAAAAGATATCAGTGAAATTAACCAAAAAGGTTTAGCTAAACTTAAAGAAAAATATGGCTTAGAAATTTTTGTTTCAACTGGCCGCGCAGTAGTTGAAAAAACAAAAATGTTGGCTAGAAAATGTGGCAATGAAAATAATTTTGTTGCTTGA", "aa": "MTKELKKPKIIFIDLDGTTIDKSSKFKKDISEINQKGLAKLKEKYGLEIFVSTGRAVVEKTKMLARKCGNENNFVA", "aa_hexdigest": "e200c3365709a433716dadbf93c62d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 55, "aa_cov": 0.7236842105263158, "hmm_cov": 0.9090909090909091, "evalue": 1.2722536767304675e-07, "score": 27.63774871826172, "start": 11, "stop": 65 } ], "seq_stats": { "molecular_weight": 8578.032499999998, "isoelectric_point": 9.678396797180177 }, "id": "IGIGPGHMMC_1664", "locus": "GCA000148625_01661" }, { "type": "cds", "sequence": "contig_1", "start": 947014, "stop": 947214, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTATTATTAACTCAAACTTTAAAAATGGTTTGTACTCGAATTCATTTCTTTTTAAAGTTGCTTCAATTTTCAAAAAAGGCAAATGCTACAAACTAAAAGAATTTAATAAAGAAACTGAAGTATTTAAGTTACTTTTTCTTAATTTGTCAAAGAAAAAACTTCATTTATTTGCTGAAGAACTTAAAAAATTATGA", "aa": "MTIIINSNFKNGLYSNSFLFKVASIFKKGKCYKLKEFNKETEVFKLLFLNLSKKKLHLFAEELKKL", "aa_hexdigest": "04644b2c10858d67879e920fa9cb07b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7807.306799999996, "isoelectric_point": 9.903262519836424 }, "id": "IGIGPGHMMC_1665", "locus": "GCA000148625_01662" }, { "type": "cds", "sequence": "contig_1", "start": 947389, "stop": 947520, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGTAATTGCTATGAAAAACTCTGTTCCTGAATTTAAAGATAAAGCAGATTTTATTAGTCCTTTTACAAATAAAAAAGGTGGTTTATATAAAACTTTAGAAATGTTACTTACAAAGTTTGAATAA", "aa": "MGYVIAMKNSVPEFKDKADFISPFTNKKGGLYKTLEMLLTKFE", "aa_hexdigest": "eff5f802b51b4971c1ac375fb2db53a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4931.786999999999, "isoelectric_point": 9.100824546813964 }, "id": "IGIGPGHMMC_1666", "locus": "GCA000148625_01663" }, { "type": "cds", "sequence": "contig_1", "start": 947747, "stop": 947959, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTATTGTTGCCACTTTTGTGTGTCAAACTGTATTTTATAAATACAAAAGAGAAGGTGTGGATTACATTTTATATTCAAAACCTTTAAGTAGAACTCAAATATATTTTGCTAATATTTTTGTATCTGCAATTGGTCTATTATTTTCAATAGCAATTATCGAATTAGGTTATTTTATAAGCAGATTGGTTTATCCAACAATTATTTGA", "aa": "MIFIVATFVCQTVFYKYKREGVDYILYSKPLSRTQIYFANIFVSAIGLLFSIAIIELGYFISRLVYPTII", "aa_hexdigest": "2e51f333131899ab4915be886a604b38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8142.654399999993, "isoelectric_point": 9.20616588592529 }, "id": "IGIGPGHMMC_1667", "locus": "GCA000148625_01664" }, { "type": "cds", "sequence": "contig_1", "start": 948191, "stop": 948385, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTAAAGAAAAAGGCAACAAGAAATTCCTTGAAGAATTAAAAGGCAGATTCAATGAAAATCAACCTATCTTGATGAGTAGATACAACGATGACTTAAAAAATACTTTATTAAATATTACAGAAGATTCAGAAGATATCACAAAATATATTGACAATACTCATAAGTCAGTTTATAACTATTTATATTGA", "aa": "MPSKEKGNKKFLEELKGRFNENQPILMSRYNDDLKNTLLNITEDSEDITKYIDNTHKSVYNYLY", "aa_hexdigest": "5f242073680cef39c575dfd506138f65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7661.503400000002, "isoelectric_point": 5.727744865417481 }, "id": "IGIGPGHMMC_1668", "locus": "GCA000148625_01665" }, { "type": "cds", "sequence": "contig_1", "start": 948743, "stop": 948982, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAGTTAAAACTTTTTGAATCAATATTTTTAGATAAATTATATGAAAATATTCAAGAGATCAAATTTAACAAATTACCTTATGTTATAGATTACGAGGGCAAAAAGGTATTAGTCTTATCAAAAGTATTTCAAGACTTTAACAAAATATTAAGCTATGTTGAAGAACATCAAAAAGATAAATACAAAAACTTAAAAATTAAAACGTATATTGCAACATATTATCCACAATGA", "aa": "MIDKLKLFESIFLDKLYENIQEIKFNKLPYVIDYEGKKVLVLSKVFQDFNKILSYVEEHQKDKYKNLKIKTYIATYYPQ", "aa_hexdigest": "5d26c0ca6e516fa7d0391693782ee8a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9646.199099999994, "isoelectric_point": 9.002639198303225 }, "id": "IGIGPGHMMC_1669", "locus": "GCA000148625_01666" }, { "type": "cds", "sequence": "contig_1", "start": 949187, "stop": 949417, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCTTATGTATGGTTTAAATAAAGAACAATATGTTTTAAGTTATCAAGCTTCACCTAATAATTTGGATGAATTAAATGAATTAGACAATGCAGGTGGTATTGAATTAATTAAATTAACACCACTTGAAAGAGATTTAATTGTTGTTACAAGAAAAGATCACATGACAGTTGGCGGATCAATAATTTTATCAATGTTAGCTGCTTTTGCTATGATGGCTGGTGGTTGA", "aa": "MTLMYGLNKEQYVLSYQASPNNLDELNELDNAGGIELIKLTPLERDLIVVTRKDHMTVGGSIILSMLAAFAMMAGG", "aa_hexdigest": "3da7af128e9fc4b9501d01fd990d5ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8301.588199999998, "isoelectric_point": 4.597785758972168 }, "id": "IGIGPGHMMC_1670", "locus": "GCA000148625_01667" }, { "type": "cds", "sequence": "contig_1", "start": 949873, "stop": 950337, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1", "UniRef:UniRef90_C4XFP1" ], "nt": "ATGTCAAATTTAAAACCTGAAGAAGCAAAAGAAAAAGTAAATTTCTTATTAAAAAAATATCACTTTGATGGACCTGAATTTGAAAAATCAGCAGAGCAACTTTCATCAGGCCAAAAGAAAACAATTTTATTAATGCAAGCTTTATTAAATGATCCTGAAATTTTAATTATGGATGAACCTGCTGCTAATTTAGACCCAAGTGCTAGAGTGATATTTTATGAACAAATAAAAGAATTAAGTTTACAAGGAAAAACTATATTTATTTCTAGTCATATTCTTCTAGAACTTGAAAGATATATCGATTCTTTTACGGTGCTTGCTAAGGGCAAAGTACTAGACAATGGAACAATAAAAGAAAAATTGCACAATGCAAAATATAACAAAAAAATAAATACAAATGATAACAATAAAATAGTGGAATATTTAATTAAAAACCGAATTGATTTTATTGAAAAACATGATTGA", "aa": "MSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHD", "aa_hexdigest": "ce46563bf145cbb5fce075b9339ef461", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 949573, "stop": 950475, "observations": { "mutation": [ 950335 ], "stop": [ 950335 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP1", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 295.0, "evalue": 2.66e-98, "gene_start": 1, "gene_end": 154, "reference_start": 144, "reference_end": 297, "reference_sequence": "MMKTLVKKSKWRIYLEKRANYKATIKRAKNYEYQQPQEDVAISIQHLHKFFKLGHGDFKTALDDVCFDVKKGSFHGFIGDNGAGKTTTIRSILGFYPSKIGKIFINGIDSTNKKAKSFIGYIPEVSLFPRKVKVKEYLLAFAEMSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHDWINAKLDEEQVNKFLQYCLKNNIQIYNLADNKLSLNELYFNAENVE" }, "paralog": false, "identity": 0.9933333333333333, "score": 557.0, "evalue": 4.47e-204, "description": "Internal stop codon at 950335. Nonsense mutation around 950335.", "psc": { "uniref90_id": "UniRef90_C4XFP1", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP1", "UniRef:UniRef50_C4XFP1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1" ], "product": "ABC transporter domain-containing protein" } }, "id": "IGIGPGHMMC_1671", "locus": "GCA000148625_01668" }, { "type": "cds", "sequence": "contig_1", "start": 950533, "stop": 951306, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTCACACAAGATGAATACAACAAAGTTGAATCTAATAAAAATATCATTAATTTTGAAAAAGCAAGAGAAGAAATTACTGAGAAATATCAAATTGAACATCTTGAATTAATTGAGCAATTATTCAAAGAATCATACTTAGGTTTAGACTGTCAATATTACATGAATGATAGTCATCAACTTTCAAGTGAAATCAAATTAGATAATTCAGATGATGATGAAAATGGTGGCGAAAAAGAAGTTGACTTAAATGTCGAAGTTCAAAATGTTGATAATCTTGAACAATTACAAGCAGAAGCTCAAAAAGTTGTTGATTTAGAAAACTTGGCTAACTTTGAAGAAGCTGAAATTGAAGAAAAGAAAAATCAAAAGGTTGAAACTCAAAAGATAGAAGAAGATGCAAAAGAAACAATTACAGAAATTAAGGAAGAAGTTGTTCCTGAAACTAAAGTTGAAGAACAAAATATTAAAGTTGAAGAAGTTAAAAATCCTTCTGCACAACAAGCTCAACCAAATCAACCTAAATTCAATTTAAATAAACCTCAAGATCAATACCAACAAACTTCTAGACTTCGCCAAGTAAATCCAAATATGTATCAAAATGTTCAATATCCAAACCAATATCAAAACTTTCCAAATAAAGGCTATGTTGATCCAAGATTTGCACAATATCCTCCTAGACAAAATCCATATTATCCAAACCAAAATTATACAAACATAAATGTAGCTCAAAGACAACCACAAAATCCTAATAATCCTATAAAACAATAG", "aa": "MDFTQDEYNKVESNKNIINFEKAREEITEKYQIEHLELIEQLFKESYLGLDCQYYMNDSHQLSSEIKLDNSDDDENGGEKEVDLNVEVQNVDNLEQLQAEAQKVVDLENLANFEEAEIEEKKNQKVETQKIEEDAKETITEIKEEVVPETKVEEQNIKVEEVKNPSAQQAQPNQPKFNLNKPQDQYQQTSRLRQVNPNMYQNVQYPNQYQNFPNKGYVDPRFAQYPPRQNPYYPNQNYTNINVAQRQPQNPNNPIKQ", "aa_hexdigest": "1458df4c6c86c3b26ebe3a48c7d19f9c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30352.7891, "isoelectric_point": 4.50729808807373 }, "id": "IGIGPGHMMC_1672", "locus": "GCA000148625_01669" }, { "type": "cds", "sequence": "contig_1", "start": 951391, "stop": 951537, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATGAAAGCAAAGAAAAAGTATTTGCCAACATTAAATTAGATGGTAAAGCAATTACAGTTAAATCACTTGCATATTTATTAACCTTTAATTTATGTTCACAATTAAACTTAGATATGAATTTAACTGAATCGTTCTACTGA", "aa": "MENESKEKVFANIKLDGKAITVKSLAYLLTFNLCSQLNLDMNLTESFY", "aa_hexdigest": "0c204871d5679cf8e28c8693b158fae8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5489.296399999999, "isoelectric_point": 5.05215663909912 }, "id": "IGIGPGHMMC_1673", "locus": "GCA000148625_01670" }, { "type": "cds", "sequence": "contig_1", "start": 951592, "stop": 952074, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTCAGATATCCATAACTTCAGAAGCACTAACACAATGGGTGATGATATTGCAGAAGAATTCAAAAAAGATTACAACAATAAATTTGAAGAAATTGTTAAATATGTAAACAGTTTATTGCAAAATAATGTATTTAGCAAAATCAAAAATCAAAACAATTTAGCTTTAAATGCATTCAAAGCTTTAAAACAAACATTAGGTGAAAACAACAAGATAGCTCAAGAATTAGCTTATGCAGGTTGTTCAATTCACTATCAAGAAATTGGTTTTGATCCTTACATTGAAAGTCAATTAGATTTATTAAGAAATAATTCAGATCTTGAAATATTCTTTAAAACAAAAAAACCTAATGAAAGTAGATATTTAATTACTTATGTTCGTAAAGCATTGAAAAACAATAGAAGTAAATTATTTGCATTCTTAATTTACAAAAACTTATTCTCACATGAATACAGCAACCTATTAGAAGAAAAAGGTTGA", "aa": "MYSDIHNFRSTNTMGDDIAEEFKKDYNNKFEEIVKYVNSLLQNNVFSKIKNQNNLALNAFKALKQTLGENNKIAQELAYAGCSIHYQEIGFDPYIESQLDLLRNNSDLEIFFKTKKPNESRYLITYVRKALKNNRSKLFAFLIYKNLFSHEYSNLLEEKG", "aa_hexdigest": "d930a71b45af3f18d3eb8310c2a904c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18801.042700000005, "isoelectric_point": 8.48785915374756 }, "id": "IGIGPGHMMC_1674", "locus": "GCA000148625_01671" }, { "type": "cds", "sequence": "contig_1", "start": 952129, "stop": 952641, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGTTGGATTCTTAGCAATTGCTATTTATTATGGTAGAAACTATTTATTAGACTTAAAAAAATCAAAAAATATAGTAGTTCAAGAAACTAAAGAAATAGTAGTTATTGAAGATAAAGAAAAACTTGTTAACTTAATTGACAAATCTGGCTTAGATGCTTTGATTATAGAAGATTTAGGCGAATCTAAAGATTATCAATTAATCAAAAGAATTAAAGATATTAACACACTTGTAAACAAAAGAAGACAAAAAAGAATTGAAAAAATTTTAGCTAAACTTGAAAATAAAAAAGAACAAATTGCTAAATTTTATGCATCAAAATACTATGTTAAAAAATGTAAAGCAATTAAATTCTTCTATGTAATTGACAACACACTTGCATCACTAGAAGACAAAGGTAATATTTTTGATTTTTCATTAACTGTTTTACCAATTAGCAAAGAATACAAAAAAGAACAAATCAATAAATTGTTATCACAATCAAATTTAAGTGTTAAAGAATTAAGCTGA", "aa": "MIVGFLAIAIYYGRNYLLDLKKSKNIVVQETKEIVVIEDKEKLVNLIDKSGLDALIIEDLGESKDYQLIKRIKDINTLVNKRRQKRIEKILAKLENKKEQIAKFYASKYYVKKCKAIKFFYVIDNTLASLEDKGNIFDFSLTVLPISKEYKKEQINKLLSQSNLSVKELS", "aa_hexdigest": "f535557940026773864f16059ccecbb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19756.0293, "isoelectric_point": 9.457657051086429 }, "id": "IGIGPGHMMC_1675", "locus": "GCA000148625_01672" }, { "type": "cds", "sequence": "contig_1", "start": 952786, "stop": 953208, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACAAAATAATAATTCTTCTTCTTTTACAAATCACCAATGTATTAGAAATGGAAACATCCAAAATAAAGTTCAAAATGAAGAACATATTATTGAAACTATAGAGCTTTATTCAACTGAAGACATGAAAAATTCTAATGCTTTCAAGTTATATAAAAAATCACTTGATTTTGCAATGAGTAACAACAATGACAAAGTGAATAATATTGCTATTATAGGTTCTCAAGATGGACAAAATCAAAATGTTATTGAAACATATAAATCAAGTAGAAAAGATTTTAATATAGCTAATGTTTCATTAGTAAACACAAATAATGAATTATTAGAATCAGAAATCATTAATCAAATTTCAGGCCAAGTTACTAAAAAGAATGTTAATCAAGCAAATAATAAAACAAGTGTAAAACATGTTGTATTATGA", "aa": "MSQNNNSSSFTNHQCIRNGNIQNKVQNEEHIIETIELYSTEDMKNSNAFKLYKKSLDFAMSNNNDKVNNIAIIGSQDGQNQNVIETYKSSRKDFNIANVSLVNTNNELLESEIINQISGQVTKKNVNQANNKTSVKHVVL", "aa_hexdigest": "6cd09d9c7ed27f7fe2956c03e20bf125", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15806.286599999989, "isoelectric_point": 6.828090858459473 }, "id": "IGIGPGHMMC_1676", "locus": "GCA000148625_01673" }, { "type": "cds", "sequence": "contig_1", "start": 953557, "stop": 953958, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33", "PFAM:PF12706.13" ], "nt": "ATGAATCCTACAAGATTAATACCTATTGGTGGAGTTCAAGAAATTGGTAAAGCTGCACTAATAGTTGAATACAAAAACCAAATATTTATTATTGATGCAGGTATCAAATTTGCTGATACAGCTTTAACTGGTATTAAAGGTATTATTCCTGACTATACATATTTGGCAGAAAGATATAAAAATATTGAAGGTCTTTTTATTACTCATGGTCACGAAGACCACATTGGTGGTGTTATTCATTTAATGAAACAAGTAGCAGTTAAAAAGATTTTTGCACCTAGAATTGCTATTCAATATTTGAAATTAAAATTCGATGAAAATAAAATTAAAAACAAGGTTGAATTTATTGAAATTGAAAAAAGTGCTGTACACACTTTTGGCGAATGTTCAGTAGATTTTTGA", "aa": "MNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDF", "aa_hexdigest": "e42671c62ea5b72bd2352300067aefc8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 78, "aa_cov": 0.5864661654135338, "hmm_cov": 0.9102564102564102, "evalue": 3.8296690662668427e-10, "score": 36.02237319946289, "start": 19, "stop": 96 }, { "id": "PF12706.13", "name": "Lactamase_B_2", "length": 80, "aa_cov": 0.5714285714285714, "hmm_cov": 0.9875, "evalue": 8.622297931967727e-09, "score": 31.275922775268555, "start": 55, "stop": 130 } ], "seq_stats": { "molecular_weight": 14972.367099999985, "isoelectric_point": 7.844658088684083 }, "id": "IGIGPGHMMC_1677", "locus": "GCA000148625_01674" }, { "type": "cds", "sequence": "contig_1", "start": 954022, "stop": 955119, "strand": "+", "frame": 1, "gene": null, "product": "Ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27", "UniRef:UniRef90_C4XFN8" ], "nt": "ATGTGTACCGGTGACTTTAGATTTGATTACAACCCTATTGGTGAAATTTATACTGATTTTGCTCGTTTAGATCAAATTGGAAAAGAAGGCCTAACTGTTCTTTTGTCAGACTCAACTAATGCTATGCGTCCTTTCCACTCGCCTAGTGAAAATGACATCTTAACAGATATTGAAAAATACATGCGTCAAGCTACAAGAAAAACTATTATTACAGCTTTTGCTTCAAACTTAACTAGAGTTACAGCAATTATTGAGTTAGCTGTTAAACTTGGCAAAAAAGTTGCAGCTTTTGGTCGTTCAATGGTTCAAGGAATCAAAATTGGACGTAAATTAGGTTACATTAAAGCGCCTGCAAGTGTTTTTGTTGATAAAAAAGATGTATCAAAAGTTCCTGACAAAGATCTTGTTATTCTTACAACAGGTTCACAAGGTGAACAATTGGCTGCTCTTTCAAGAATGTCTTATGGTAAACACCAAAGCGTAAAAATTGAAAAAGGTGACATGATTATCTTTTCATCAAGTCCAATTCCAGGGAATAGAATGGTTATTGAACTTTTAGTTAATAGACTTTCAAAACTTGGAGCTATTATTAAAGAAAATGGTGTTGATGGTTACTTGCATACCTCAGGTCATGCTTACAAACACGAACATGATAAAATTTTCCAACTTACTAAACCAAAATACTTTTTACCTTACCATGGTGAATTCAGAATGAGTATAGTTCATGGCCAAAGCGCTATTAGAAATGGAGTTAAACCAGAAAATATAATGATTCCTGAAATTGGTCGTGTTTACAACATGATTAATGAAGAAATTACACAAACAGATGAAAAAATAGATTGTGGTCCTATTTATATTGATGGACACACAACCTTGAATTTAAGTGCAAATATACTTAAGGAAAGAGCTAAATTAGCTGATAGCGGTTTTGTAAATATGATCCTAAATATTAACAAAAAAACTAATTCAATAGTTGGCCGTCCAAGTTTAATTAGTCGTGGGAGCTTCTATGTAAAAACTTCGCTTGAATTAGTTGAAGAAGCTAAAAGAATTGCTCATGGTGCTGCTCTTTTCTATATTAAAAATACACCTGATTGA", "aa": "MCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPD", "aa_hexdigest": "a545fda8d8fe4ce8f64bce770d4f7502", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 953722, "stop": 955398, "observations": { "mutation": [ 953956, 955117 ], "stop": [ 953956, 955117 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFN8", "query_cov": 1.0, "subject_cov": 0.5667701863354038, "identity": 1.0, "score": 716.0, "evalue": 3.3e-256, "gene_start": 1, "gene_end": 365, "reference_start": 187, "reference_end": 551, "reference_sequence": "MYFYIFIIILHNRSIKVLLAVFLNIKKGDQNMNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDFWTAQHSIPDAFGIRIKTPNGSIMCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPDWNIVDLKQLIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE" }, "paralog": false, "identity": 0.996415770609319, "score": 1092.0, "evalue": 0.0, "description": "Internal stop codon at 953956, 955117. Nonsense mutation around 953956, 955117.", "psc": { "uniref90_id": "UniRef90_C4XFN8", "product": "Ribonuclease J", "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFN8", "UniRef:UniRef50_Q6KH27" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27" ], "product": "Ribonuclease J" } }, "id": "IGIGPGHMMC_1678", "locus": "GCA000148625_01675" }, { "type": "cds", "sequence": "contig_1", "start": 955141, "stop": 955398, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTATTGATCGTCTTGAAAATCTTTTCTACAAAGAAAAAAGAAGAAGACCAATTGTTGTGGCAACTATTCTTTTTACAGATGAAGAAAATGAACCAGCATTTGCAACTTCTAAAATTAAATTTAATGTTAAGGGTACAAAAGAAGATGAGAAAAGCAAAAAAGCTACTGCTGCTCTTAAAGAAGCAAAATCAGAAATGTTTGGTGAAAACAGTGAAATGTCAGAAGACATTCATGATGAAGAAGACGAAGAATAA", "aa": "MIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE", "aa_hexdigest": "3f4bd52e31ced8ad13e37a6918a462b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9870.935000000003, "isoelectric_point": 4.824345970153809 }, "id": "IGIGPGHMMC_1679", "locus": "GCA000148625_01676" }, { "type": "tRNA", "sequence": "contig_1", "start": 955479, "stop": 955554, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 75.9, "nt": "GCACCGTTAGCTCAGTCGGTAGAGCAGCTGGCTCTTAACCAGCGGGTCACAGGTTCGAACCCTGCACGGTGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 955519, 955521 ], "id": "IGIGPGHMMC_16", "locus": "GCA000148625_01677" }, { "type": "cds", "sequence": "contig_1", "start": 955856, "stop": 956242, "strand": "-", "frame": 1, "gene": null, "product": "DUF4064 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355056.1", "SO:0001217", "UniParc:UPI0001A8C3E2", "UniRef:UniRef100_C4XFN7", "UniRef:UniRef50_C4XFN7", "UniRef:UniRef90_C4XFN7" ], "nt": "ATGAATAAAAAAAGTTTTAAATCAACTTTAACTTCAATTGCTATAGCAGCAATTTTATTATTAACACTAACTATCTCATTTTTTGGATTACAAAAAAATATCCCAGAAGCAGTAATAAAAGCGACAAATAACAATTATGTGGCTGGAATTACTAACATTATTTTATTAGTTATTACTCTTGCTCTATGCATTTCTTTTATTGTTGTTGGTTCAATTCAAGCAGCAAAATTAAGTTTTCTTAAAAAAGATCGTTACATTTATGTTTTAGCAATTTTAGGAATTTTCTTTGTCCCTGTTTTCTATGTAGTAGCAGGAATTATTCTTGCTAAAAGAGATTCTATTGTTAATGAATTAGTTGATGAAGAAAATGAAGCAATTGAAGTTTAA", "aa": "MNKKSFKSTLTSIAIAAILLLTLTISFFGLQKNIPEAVIKATNNNYVAGITNIILLVITLALCISFIVVGSIQAAKLSFLKKDRYIYVLAILGIFFVPVFYVVAGIILAKRDSIVNELVDEENEAIEV", "aa_hexdigest": "fb0c22528d5377771443af7c318b9197", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C3E2", "ncbi_nrp_id": "WP_013355056.1", "uniref100_id": "UniRef100_C4XFN7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3E2", "RefSeq:WP_013355056.1", "UniRef:UniRef100_C4XFN7" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN7", "uniref90_id": "UniRef90_C4XFN7" }, "psc": { "uniref90_id": "UniRef90_C4XFN7", "product": "DUF4064 domain-containing protein", "uniref50_id": "UniRef50_C4XFN7" }, "pscc": { "uniref50_id": "UniRef50_C4XFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN7" ], "product": "DUF4064 domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1680", "locus": "GCA000148625_01678" }, { "type": "cds", "sequence": "contig_1", "start": 956442, "stop": 957044, "strand": "+", "frame": 3, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "RefSeq:WP_013355057.1", "SO:0001217", "UniParc:UPI0001E33087", "UniRef:UniRef100_UPI0001E33087", "UniRef:UniRef50_A0A3P8KMF6", "UniRef:UniRef90_A0A3P8KMF6" ], "nt": "ATGGCAAAATCAAAAGCTGATATTGTCCGTGTTCGTAAGCTACAATTCATCGCTGGCCAAGCCAAACCTGGTCCAGCTTTAGCTGGTGTTGGAATCAATATGCCTGATTTCACACGTGCTTTTAATGACGCAACACGTGATAGAGGTAATGAACCAGTTCCTGTTGAAATTACCGTTTACAAAGATAAATCATTTGACTACAAGTTATTTACAGCTCCAGCTTCGTACAAATTAAAGCAAGCTGCTAAATTAAACAAAGGTAGTTCAAACTCAAAAACAACAATTGCTGCTACTATTACAAAAGAACAATTGAGAGAAATTGCTGAATACAAATTACCAGACTTAAACACAAATGATGTTGAAGCTGCTATGAGAACTATTGCTGGAACAGCAAGACAAATGGGTATTATTATCGAAGGATTTGATAACGTTGCTGCTAAAAAAGCTGCTGCTGCTAAAGCTGCAAAAGACAACGCAGCTGCTGCAGCTAAAGAAGCCGCTTTAGATGAAGCTCAAAACGAACTAAAAGAATCTAAAGGTAAAACCATTGATGTTAAAACAATTTCAGACGAAAAAGCTGCTGCTAAAGAAGGAGAAGAATAA", "aa": "MAKSKADIVRVRKLQFIAGQAKPGPALAGVGINMPDFTRAFNDATRDRGNEPVPVEITVYKDKSFDYKLFTAPASYKLKQAAKLNKGSSNSKTTIAATITKEQLREIAEYKLPDLNTNDVEAAMRTIAGTARQMGIIIEGFDNVAAKKAAAAKAAKDNAAAAAKEAALDEAQNELKESKGKTIDVKTISDEKAAAKEGEE", "aa_hexdigest": "8d34c48cdc10ada1e9b82378c16c9156", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E33087", "ncbi_nrp_id": "WP_013355057.1", "uniref100_id": "UniRef100_UPI0001E33087", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33087", "RefSeq:WP_013355057.1", "UniRef:UniRef100_UPI0001E33087" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33087", "uniref90_id": "UniRef90_A0A3P8KMF6" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KMF6", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_A0A3P8KMF6", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KMF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KMF6" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "IGIGPGHMMC_1681", "locus": "GCA000148625_01679" }, { "type": "cds", "sequence": "contig_1", "start": 957044, "stop": 957739, "strand": "+", "frame": 2, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_013355058.1", "SO:0001217", "UniParc:UPI0001E33088", "UniRef:UniRef100_C4XFN5", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_C4XFN5" ], "nt": "ATGTCTCTTAAAGGTGGAAAGAAAATAAGAAATGCTCGTGAATCATTTGACAAAACAGTTGCATATGATTTAAAAGAAGCATTAGAAATTGTTAAAAGAACATCATTTACAAAATTTGTTCCTACAGTAGAATTAGTATTCAAATTAAACCTTGACGTTAGAAAAGCTGACCAACAATTGCGTGGATCAGTATTGTTGCCTAACGGTACAGGTAAAAGCGTTCGTGTATTAGTTGTTTCAAACAATCCAGCAATTCAAAAATCATCAAAAGCTGCTGGTGCAGACATCGTTGTTGATGGTCCAGCACTTGAACAAAAAATTAAAGAAGACGAATTTGACTTTGATGTTATGGTTGCTGACCCTTCAATGATGCCTTTATTAGGTAAATATGGTAAAAAACTTGGACCTAAAGGTTTAATGCCAAACCCTAAAACAGGTACAGTTACACCTACTCCTGATAAAACAGTTGCTGAACTTAAAAAAGGTAAAGCTAACTACCGTACAGATAAAGCAGGTATTGTACATACAATCATTGGTAAATTAAATATGACAACAGACCAATTAGCAGAAAATGCTCAAACAATCATTAATTTAATTAAAAAACTTAAACCAGCTACAGTTAAAGGTGCTTACATGCAAAACTTAGTTGTAACATCAACAATGGGTCCTGGTGTTAAAGTTAAAATTGAAAAATAA", "aa": "MSLKGGKKIRNARESFDKTVAYDLKEALEIVKRTSFTKFVPTVELVFKLNLDVRKADQQLRGSVLLPNGTGKSVRVLVVSNNPAIQKSSKAAGADIVVDGPALEQKIKEDEFDFDVMVADPSMMPLLGKYGKKLGPKGLMPNPKTGTVTPTPDKTVAELKKGKANYRTDKAGIVHTIIGKLNMTTDQLAENAQTIINLIKKLKPATVKGAYMQNLVVTSTMGPGVKVKIEK", "aa_hexdigest": "54a24d6dbe97b10a979f8eab9baf07d5", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33088", "ncbi_nrp_id": "WP_013355058.1", "uniref100_id": "UniRef100_C4XFN5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33088", "RefSeq:WP_013355058.1", "UniRef:UniRef100_C4XFN5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN5", "uniref90_id": "UniRef90_C4XFN5" }, "psc": { "uniref90_id": "UniRef90_C4XFN5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "IGIGPGHMMC_1682", "locus": "GCA000148625_01680" }, { "type": "cds", "sequence": "contig_1", "start": 957827, "stop": 958336, "strand": "-", "frame": 1, "gene": null, "product": "Aminotransferase class V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3", "UniRef:UniRef90_A0AB32XD79" ], "nt": "ATGCCTGGTTTAAGTTGTGTTGTTTTATCTAAAAGAGCTCAACAAGTAGTTAAAGCAAATAAAAACTTTAAATTCTATTTTGATTTATCAGCTTATATTAATTTTTATGAAAGAGAAAAATCAACTCCTTATACTCCAGCTTCAGGTATTTTATTAGCTTTGAATGCTGCAATGAAAGTTATCAAAAACCAAACATTTAAGAATATAAGAAATAATAAACTCAAAATATATGACTACATTAAAAAAGAATTAATTGCTATTGGATTTAAGGATGTAGTTCCTAAAAAGAATATAACTATTGGACTTTTAGTTTTAAATGTTCCAGAAGGTGTTGATGCAATTAAGTTAAGAAATAAAATTGAATATAATCACAATATTTATTTTGAATTAGGTCGTGGAAATAGAAGAAGTAAACAAGTCAGAATTGGAATTCCTAATACTATAGACATGGAAAAAGCTAAAAAACTTGTTAAAGTAATTAAGGAAACTTTAAAGATTAAAATTGATTAA", "aa": "MPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID", "aa_hexdigest": "f1da88a11b553b96b404e2e7f0324978", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 957827, "stop": 958636, "observations": { "mutation": [ 958384 ], "stop": [ 958384 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD79", "query_cov": 1.0, "subject_cov": 0.49415204678362573, "identity": 1.0, "score": 327.0, "evalue": 1.68e-110, "gene_start": 1, "gene_end": 169, "reference_start": 174, "reference_end": 342, "reference_sequence": "MLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDNWGLDVAIATSGKAFCVMPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID" }, "paralog": false, "identity": 0.9962825278810409, "score": 528.0, "evalue": 2.02e-192, "description": "Internal stop codon at 958384. Nonsense mutation around 958384.", "psc": { "uniref90_id": "UniRef90_A0AB32XD79", "product": "Aminotransferase class V", "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD79", "UniRef:UniRef50_A0A3P8MEM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3" ], "product": "Soluble hydrogenase 42 kDa subunit" } }, "id": "IGIGPGHMMC_1683", "locus": "GCA000148625_01681" }, { "type": "cds", "sequence": "contig_1", "start": 958382, "stop": 959017, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGAGATTGGAAGCAATTTCTTCAAATTTGATTAAAGAACTTGACAATTTTCAAGACTTAATTAATATTGATCATCATTATGCAAACCACGCTTCTACTTTATTATTTACACCAGGTCCTATTAATAATTATCCAGAAGTTTCTGAGATTTTATCTGAGTCAGTGTTGCATCATAGAAGTGAGTTGTTCAAACACTACATGGAAGAATCAACTAATTTAATTAAAGAATTCTTTAGAACTAAAGAAGCACTTCCATTATTTATTACATGTTCAGCAACTGGGGCTATGGAAGCAGTTGTTGTTAACCTTATTAGTCCTGAAAACAAAGTTTTATTGGTTGAAGCAGGTGACTTTGGTAAAAGATTCCAAATAATTTTAGAAAGATTAATTGGAGCTAATAATCTTGATGTTATTCATTATGAAGATGGTGAAGCATTCAAAGTAAAAGATATAGAAAATAAACTTAAAGAAAATAAATATGATGCATTCTTCTGTACTCATCATGAAACATCAACAGGTGTTTTAAATGATATTGAAGCTATTTCAAAAGTAGTTAAAAAATATGCTAAAGATACATTATTTATTGTGGATACAGTTAGTTCATTTATTCATGAAAATGTTGAGTTTGATAATTGA", "aa": "MRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPINNYPEVSEILSESVLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDN", "aa_hexdigest": "d7889436650376bf54f1bb44a5349ef5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 160, "aa_cov": 0.7393364928909952, "hmm_cov": 0.975, "evalue": 1.3252735955488384e-15, "score": 53.21034240722656, "start": 54, "stop": 209 } ], "seq_stats": { "molecular_weight": 24076.008600000026, "isoelectric_point": 5.088192558288574 }, "id": "IGIGPGHMMC_1684", "locus": "GCA000148625_01682" }, { "type": "cds", "sequence": "contig_1", "start": 959219, "stop": 959641, "strand": "-", "frame": 1, "gene": null, "product": "Nucleotidyl transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2", "UniRef:UniRef90_A0AB32XDA2" ], "nt": "ATGGCAAAAGTTAGAAATGCTGTTATTTTTGCAGCAGGTAAGGGGACTAGAATGACCCCATTAACTCATTATGTTCCTAAACCATTAATAACTGTAAATGATGAATCAATGATTGAAAGAAACATAAAGCATTTACAAGAAATAGGCATTAATGACATCACTGTGGTTGTTGGTTATATGAAAGACCAATTTAATTTAATTAAAGAAAAATATAATGTCAAATTAATTGAAAATGATTTATATGATGAAGCAAACAACATTTATTCTTATGTTGTTTCCAAAAATGCATTTGGAGACACTTTATATATTGAAGGTGATTTGTATATTGAAAAAAATATTTTTCCAGAAATAATTAAAATAATTGAAAACTCTGAAGATTCAATTTGTTTTACTGAAAAATGTACAAAACACAAATCGGAATGA", "aa": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSE", "aa_hexdigest": "723778bd553b296ea32e140b7f45037c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 958916, "stop": 959641, "observations": { "mutation": [ 959035, 959083, 959146, 959221 ], "stop": [ 959035, 959083, 959146, 959221 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDA2", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 281.0, "evalue": 2.09e-94, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSEWVFDTNNKGYVVKHHNEKDAYNQNIWIGILYLNKKLANEAKEKVDAWFNQQGNKQQYFETFLWTLENKMRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPIK" }, "paralog": false, "identity": 0.983402489626556, "score": 473.0, "evalue": 2.73e-172, "description": "Internal stop codon at 959035, 959083, 959146, 959221. Nonsense mutation around 959035, 959083, 959146, 959221.", "psc": { "uniref90_id": "UniRef90_A0AB32XDA2", "product": "Nucleotidyl transferase", "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDA2", "UniRef:UniRef50_A0AB32XDA2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2" ], "product": "Nucleotidyl transferase" } }, "id": "IGIGPGHMMC_1685", "locus": "GCA000148625_01683" }, { "type": "cds", "sequence": "contig_1", "start": 959691, "stop": 959861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTCTTTTCGATATTAGGTGATTTATACTTTTCATATGTTAAAAGAATAAATGCGATAAAAGATTATTCAAAAATTTTAATAAGTCATGGTGGTATTTTAGATCGTTTTGATAGTGTTGCTTTAGGAGGATTAACTTTTCTTTTGTTGTTTGTTATATTTATATAA", "aa": "MPFFSILGDLYFSYVKRINAIKDYSKILISHGGILDRFDSVALGGLTFLLLFVIFI", "aa_hexdigest": "16c6ce4dbdaf0ed75b8b19a0bce215ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6359.517399999997, "isoelectric_point": 8.1192289352417 }, "id": "IGIGPGHMMC_1686", "locus": "GCA000148625_01684" }, { "type": "cds", "sequence": "contig_1", "start": 960102, "stop": 960254, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTGTTGCTTTTATTATTATTGAATATATAACAAGAAATAATATGAGTTTTGAAGATCGTTTGGTTAGAGGAATACTAATGTTTATTTCTCTATACGTAATAGTCAACACTTCAAAATTGATTCAAATAACCATTATACTTGATTGA", "aa": "MISVAFIIIEYITRNNMSFEDRLVRGILMFISLYVIVNTSKLIQITIILD", "aa_hexdigest": "985fba476d0b234caad08870561bcc04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5834.993599999998, "isoelectric_point": 6.045929527282715 }, "id": "IGIGPGHMMC_1687", "locus": "GCA000148625_01685" }, { "type": "cds", "sequence": "contig_1", "start": 960276, "stop": 960389, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATACTACCTACTGTAGTTTTACCGGTTAATAACTTTACTGAGTTTATTTTTGTTTCTCATCCTTTAGAATTTCATCCTAGTTTTATTCAAGAAATAGCAAAAGAATGA", "aa": "MLILPTVVLPVNNFTEFIFVSHPLEFHPSFIQEIAKE", "aa_hexdigest": "ad9ae4d054a84fca641b04350f50ab53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.0075, "isoelectric_point": 5.015893363952636 }, "id": "IGIGPGHMMC_1688", "locus": "GCA000148625_01686" }, { "type": "cds", "sequence": "contig_1", "start": 960411, "stop": 960533, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACCTTTAGCAATTTTTGGAAAAAATCATTATGAAGCTAGAATAGTTTCATATATTTTTACTGCTATTGGGATAGGAATACTATGCTATGAATATTTCAAAGCTAATCATTTAAAATGA", "aa": "MVPLAIFGKNHYEARIVSYIFTAIGIGILCYEYFKANHLK", "aa_hexdigest": "887ccf7e2e2e6a56cadadfc2068deb6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4605.4251, "isoelectric_point": 8.932368659973147 }, "id": "IGIGPGHMMC_1689", "locus": "GCA000148625_01687" }, { "type": "cds", "sequence": "contig_1", "start": 960718, "stop": 961803, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355061.1", "SO:0001217", "UniParc:UPI0001E3308B", "UniRef:UniRef100_A0AB32XDL5", "UniRef:UniRef50_C4XFN2", "UniRef:UniRef90_C4XFN2" ], "nt": "GTGTCTGAAGAATTAGAAAACAAAGAAAAAAAAGTTAAAAAAGATCAAACTTCTACTAAAAAATCAACAGCCAAAAAAATGACCAAAAAGTCTGCAAAAAAGGCTGAAAGAGAAAAAAATAAAAAAGAAGAAACTGGTCTTTTACAAGCTATTATTGAAGAAGAAAAACTTCTTGAAGAACAAGTCGCTGAATTAGAAATTGACAAAAATATCAAAATTACTAAAGGCAATGTTTTTGAGCTTTTAGACAGAGATAGTTCTAAAAAGAATATTATTGTTCCTAAAAAAGTTCGAAAATTATTGACTAAGTTAGCTAAGAAAAAAAGAAAAAAAGATGGCAATGAAAACCTAAAAAATGAAGAAGGTAATGTTATTGAAGTTAAGAACGTTAGCAAATATTATGTTAATGGTAATATTGTTACAAGAGTTTTAAAAGATGTTTCAATTGAAATTAAAAAAGGTGAGCTAATGCTTATTTATGGTGTTAGTGGTGGTGGTAAAAGTACACTTCTAAACCTTATTTCAGGACTTGATAGACCTTCAAAAGGACAAGTTATTGCTTGCGATAATAATCTTCCTTATATGTCTGATAGTCAATTAACTCTATTTAGAAGAAAACATGTTAGTTTCATTTTCCAAAACTATAACTTGTTAGCAAACTTAAATGCTTTTGACAATGTTCAAACGGGAGCTTACTTACAAAAAAATCCTGCTAAAAAAGTTGATGTTGTTGAGTTATTTAAGAAATTTGAAATGGATGAAGAAATATCAAAATTCCCTGCTCAAATGTCTGGTGGTCAACAACAACGTGTTTCAATTATGAGAGCCTTAGCTAAAAATGCAGATATCATTTTTGCTGATGAACCTACTGGTGCTCTTGATGAAGCAACAACAAAAATAGTTTTAAGATTTCTTTACAATATTAATAAAGAAACAGGCGCTACAGTTGTTATGGTTAGTCATAACCCAGTTATGGCGCAAATGGCTGATAGAATTATTCATGTTGTTAAAGGAAGAATTGCAAAAGTTGAAGTTAATCAAAAACCAATTCACCCTGATGATATTGAACTTTTTAAAGAAAAATAA", "aa": "MSEELENKEKKVKKDQTSTKKSTAKKMTKKSAKKAEREKNKKEETGLLQAIIEEEKLLEEQVAELEIDKNIKITKGNVFELLDRDSSKKNIIVPKKVRKLLTKLAKKKRKKDGNENLKNEEGNVIEVKNVSKYYVNGNIVTRVLKDVSIEIKKGELMLIYGVSGGGKSTLLNLISGLDRPSKGQVIACDNNLPYMSDSQLTLFRRKHVSFIFQNYNLLANLNAFDNVQTGAYLQKNPAKKVDVVELFKKFEMDEEISKFPAQMSGGQQQRVSIMRALAKNADIIFADEPTGALDEATTKIVLRFLYNINKETGATVVMVSHNPVMAQMADRIIHVVKGRIAKVEVNQKPIHPDDIELFKEK", "aa_hexdigest": "c33a614a06c283fe2031325307294454", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E3308B", "ncbi_nrp_id": "WP_013355061.1", "uniref100_id": "UniRef100_A0AB32XDL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3308B", "RefSeq:WP_013355061.1", "UniRef:UniRef100_A0AB32XDL5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDL5", "uniref90_id": "UniRef90_C4XFN2" }, "psc": { "uniref90_id": "UniRef90_C4XFN2", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFN2" }, "pscc": { "uniref50_id": "UniRef50_C4XFN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN2" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "IGIGPGHMMC_1690", "locus": "GCA000148625_01688" }, { "type": "cds", "sequence": "contig_1", "start": 962093, "stop": 962464, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAACAAATTTATAATCCAGATACTGGAATATTAAAAACCAGATTGAATCTAACAAAAGATCAAATTATGAATTTATTAGATTCAACTGAATTAACATTTAATAAAGCTAAATATGATCAAATTAAAAATGCGCCTAAAAACGCAATAGAAAATTATGCTAAATTGTATGATTCTAAACATTATATTGCTTTAGGTACAAGTATTGATTCAAAAGATATAGAAGCTGGATTTACAACCCAAATTGGTTCTACTATAGGAACAATTAGTATTGCAATTATTGCAATTAGTTTCATAATTTCACTTGTTATTCTTATTATTATGTCAACAATTATGATTAGTGAAAATGAGAAAAACATTGCTATTTGA", "aa": "MFEQIYNPDTGILKTRLNLTKDQIMNLLDSTELTFNKAKYDQIKNAPKNAIENYAKLYDSKHYIALGTSIDSKDIEAGFTTQIGSTIGTISIAIIAISFIISLVILIIMSTIMISENEKNIAI", "aa_hexdigest": "7f65b0cc7a1b904e96c718b69efd4c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13690.737799999984, "isoelectric_point": 5.278318977355956 }, "id": "IGIGPGHMMC_1691", "locus": "GCA000148625_01689" }, { "type": "cds", "sequence": "contig_1", "start": 962519, "stop": 962878, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGTAAATGAAGTTACTTCGAAGAAATATAACATTAATATAGGCGATTTAGTCCAATTTGAAGTTCTTAACCATACAAAACGTTATGCAAACAAAGTGTTGTCAAAAATAGATAAATCATTAAGAAGTAATGTTGACTTAAAAAATCCAAAAGTAACATTTAAAGTTGTTGGTATTAACCCTACATACATTAATAATGAATTAATAACAACAGTTGATGCAGCTAATAAATTAGTTGGCTTAGACAAACTTCCAAATGTTGTTGGATTTAAAGCCTTCAATGGTGTTATGACAAATAGTCCAGCGCCTAGCCAAGTTCTTGATTCAACTTCGCTTTATTCATTAAGTGGTTATTGA", "aa": "MVVNEVTSKKYNINIGDLVQFEVLNHTKRYANKVLSKIDKSLRSNVDLKNPKVTFKVVGINPTYINNELITTVDAANKLVGLDKLPNVVGFKAFNGVMTNSPAPSQVLDSTSLYSLSGY", "aa_hexdigest": "f0482b84fd1f95324d9ceab74f8b55fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13089.918299999987, "isoelectric_point": 9.487763786315917 }, "id": "IGIGPGHMMC_1692", "locus": "GCA000148625_01690" }, { "type": "cds", "sequence": "contig_1", "start": 963122, "stop": 963277, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATATAACAACCGATAAATATCGTGATGAATTTAGAACACTCTTAATCAATGGTTATACTAAAATTGCTCAAAAAATTAAAGCCGAAAAAGAAAATCCAAGTTTAATTAAACCTAAAGAAAAGAAACCAACCAATTTATCTGATTACTGA", "aa": "MVNITTDKYRDEFRTLLINGYTKIAQKIKAEKENPSLIKPKEKKPTNLSDY", "aa_hexdigest": "fa64605c08a83b0df022737fa660076d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5985.860800000001, "isoelectric_point": 9.522189903259278 }, "id": "IGIGPGHMMC_1693", "locus": "GCA000148625_01691" }, { "type": "cds", "sequence": "contig_1", "start": 963302, "stop": 963517, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGATACTCAAAAAGCTAAAATTGATAAGATAAGAGACCGTGTTGGTTATGAGTTAGAAAGAACTCAAGAAGACAAAGATAAAGAATTCTTTGTAGATCCTAATACTTATGCTTTAAGCTTAGTAAATAAGGCTAATAAGGAAAAATTAAGCTTCTTTAAATACTATCATTCACCATATGAAAAACAAGGTAGTTTCAAATATGCTTACTGA", "aa": "MPDTQKAKIDKIRDRVGYELERTQEDKDKEFFVDPNTYALSLVNKANKEKLSFFKYYHSPYEKQGSFKYAY", "aa_hexdigest": "31a20d61db17d34b6d3ea38f3af27a1e", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8552.5075, "isoelectric_point": 8.777128791809083 }, "id": "IGIGPGHMMC_1694", "locus": "GCA000148625_01692" }, { "type": "cds", "sequence": "contig_1", "start": 963536, "stop": 963892, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAGGTATCGCAACCTTATTTGGTGTAGCCAATACAAACGTCTTTAATAGAACTATTAATGATACTTATAAAAATAGAAGTTATACATTTAAAGTTGATTTAGAAAGTCCAACAGTTGAAGGTGGAGCATTCAAACCATTTAATCCTGATAATTTATATAACAACATTTATACTCCTCTTGGTGATATAAATGAAGGTAACAGAGAAATAGGAGATTACTTTAAACCTGGTGCTTCTAGTGTGCTTAATAAAGGAACTAAAAATGGTAGTCCTCAAGATTTAGACTCACATATCTTAAGCCAATTCTCAGTTAATGTTACTGTTGCAGCTGGTGTAGCCGCTGACCCTTGA", "aa": "MLTGIATLFGVANTNVFNRTINDTYKNRSYTFKVDLESPTVEGGAFKPFNPDNLYNNIYTPLGDINEGNREIGDYFKPGASSVLNKGTKNGSPQDLDSHILSQFSVNVTVAAGVAADP", "aa_hexdigest": "f5b39e5df9c5293b34a1be4edd37b25b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12720.910799999987, "isoelectric_point": 4.898975563049316 }, "id": "IGIGPGHMMC_1695", "locus": "GCA000148625_01693" }, { "type": "cds", "sequence": "contig_1", "start": 963920, "stop": 964267, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTTTAGTTACATCAGTTATTGGTGGAATCTTAGGTTATGTAATTGGTTATAAACTACAACTGATGGTTATGAACATCTTTAGTAGTTATTGGACACTGCCAAAACAAACGATTTCATTTAACTTCTTTACATTATTCTTCACTGTCTTCTTACCATTTATTGGAATGAGCTTATTGATCTTTATAGTTTCATTATTTGCTTTAAGACATAAAGCTATAGACTTAATTAGTGGAGTTAATGAAATACCTCAATCAAAATTATTCAAAACTTATCAAAAAGCAACTAAGAAATTTAATGTTAAAAAACGTTTCTCATTTACATTGGCATTTACAAGTTTTTGA", "aa": "MFALVTSVIGGILGYVIGYKLQLMVMNIFSSYWTLPKQTISFNFFTLFFTVFLPFIGMSLLIFIVSLFALRHKAIDLISGVNEIPQSKLFKTYQKATKKFNVKKRFSFTLAFTSF", "aa_hexdigest": "0ee163dd4830ab23af7e54c735a63c13", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.757899999975, "isoelectric_point": 10.250618171691894 }, "id": "IGIGPGHMMC_1696", "locus": "GCA000148625_01694" }, { "type": "cds", "sequence": "contig_1", "start": 964289, "stop": 965431, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAATTATTTATTAAGCAAAGATTTTTATGACATTAAAGACCAAGCTCAAATCATTATGTCTTCATTAAATCTTAATGTACCATTACAAATTTTTGGTTTAAGTGAAGCCTTAGTCAATCCTATTTTAAGATTTACTTTCCCTGAAATTACATTATCTTATTTAGCAAGTCAAAAAACAAATGCTAGTTTAGTTAAAGCTAACTTACAATACTTATTGCTCAATAAAATAGATGATTTCGAAAAAATTGTTAGAAAAGGCGAAGACAAATTTTATGCCTTAACTAACATGTTAGGAACTGCATTTAGCAAAAAAGATACCTCACTAATTCCTCTTGACTTAGATGACGAACAAACATTAGTAATGGATGGAGCTACATTTGATAAATTAAAAGATACAAGCTCTTCATTATTTGGAATTGATTTTATTAAATTTGTTAATGAAGCAATGAACACGATAGTTGAACCTAAAGAAATGAAGGACATTGTGTTCTCAAATTCAAATAGTTACTTAGCTAAAGTTAACTATGCATACTTAGCAAAAAATAATAAAGCAATTTATACAGGTAAAATTCCAAGTGATCCTATTGAACTATTATCATTATTAGATAAATTAGATGAAAAATTCATTTTAAATGTTAATGGAACCAAATTTATTATTATTGGTCAAGAAACTACAGTCGACTATATGTACCCAGTTATTGATGAAAATAACTTACAAGTTAATACACAAAACCAATCCTTAGTTTATGTTAACAAATATGGTTTTGACAGAATTCACTCAGGTTACATTGGAAATGTTATTAAGAAAACTCTTTTAGTTAAAAACTCAGCAGCAACTGGTATGAATAATAATGAACTTAAAAAATTCATTACTAAAATAATAGATAACTCAATTGCAGACAGTAACAAACTTCAAAGAGTTTTCTTAGCAAATGAAATTGATCCAATTAACCCTGAAAGGTCTCTTAGAATAACAACTGTTCAAGGAATTATTAAAGCTGTTTCATCAGCTACTATTGCCTTAGTTTCAGTATTCGTTACACTTGTGGCTATTTCAACTATCTTTATTATTAAGAGATATATTTCAAATAAAAACAAAGTTATTGGTATTTTAGTGGCCCAAGGTTATCGTCCATGA", "aa": "MNNYLLSKDFYDIKDQAQIIMSSLNLNVPLQIFGLSEALVNPILRFTFPEITLSYLASQKTNASLVKANLQYLLLNKIDDFEKIVRKGEDKFYALTNMLGTAFSKKDTSLIPLDLDDEQTLVMDGATFDKLKDTSSSLFGIDFIKFVNEAMNTIVEPKEMKDIVFSNSNSYLAKVNYAYLAKNNKAIYTGKIPSDPIELLSLLDKLDEKFILNVNGTKFIIIGQETTVDYMYPVIDENNLQVNTQNQSLVYVNKYGFDRIHSGYIGNVIKKTLLVKNSAATGMNNNELKKFITKIIDNSIADSNKLQRVFLANEIDPINPERSLRITTVQGIIKAVSSATIALVSVFVTLVAISTIFIIKRYISNKNKVIGILVAQGYRP", "aa_hexdigest": "1c27aa503d074da4304bab2b68231356", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42683.90679999998, "isoelectric_point": 8.625112342834473 }, "id": "IGIGPGHMMC_1697", "locus": "GCA000148625_01695" }, { "type": "cds", "sequence": "contig_1", "start": 965549, "stop": 965818, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATGAATAAATATGCAAATCATGAAGAAGCAAAAAGATTTGTTAAAGATTTAATTGCATTAGCATTGGAACCTGAAATATTTGACATTAACAAAAAAATGTCAACAAATGACAATATACCTTTTGCTGGTGTAACTAACTATGGATTATTAGAATTACTTGAAAATCCCGAAAAATCAAACTTATTTGCTTATGATGTTAATGGCAAATTCTTAAATGACAAAGTTGAACAATTTGCTGCTAAAAATGAAATATATAGAAAATGA", "aa": "MFMNKYANHEEAKRFVKDLIALALEPEIFDINKKMSTNDNIPFAGVTNYGLLELLENPEKSNLFAYDVNGKFLNDKVEQFAAKNEIYRK", "aa_hexdigest": "df9ebb9feec64cabbf409eb29d0c0f55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10334.68269999999, "isoelectric_point": 5.366248893737793 }, "id": "IGIGPGHMMC_1698", "locus": "GCA000148625_01696" }, { "type": "cds", "sequence": "contig_1", "start": 965921, "stop": 966703, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCCTCATTAATTAGTGGTTTAAAACCTATTATTAAAAAAATGAATAGCGATCCAACAAATCCTTATTCTAATGTAAAACAAGGCTTAATTAATATTCTATCAAATATTGATTTTAATATTCTGGCTCGTGAATTAAATCAAACAATAACAACACATTATATTGATTACAAAGTAGTTAAATTTAATCCAGACTTAAACAAAGATGAAGAAAAAGTCTTTTCAGTTGTTTTAAATGCAATAAGTCCAAAAGATGGAATTATATCATTCTTAAGATCTATGTTTGGTATGCCTGGATCAAATAGAGCATTTAAAGAAAACTTAATCAAAATGTTCAATTTGTCTGACAAATATAAAGAAATAGAACTACCTAATAGTAGTGTAAAAATTTATGTTCCAGATAATGACGACACAAAAGTAGGTTTCTTTGATTTCTTAAAAATTTTTGCTTCAGGTTTATCAAATACAGAAGCTAATGAATTCAATAACTACAAAATTGAACATGACTTCTTAGCACTTAAATCATCAATTGAAAGTCTTAAAGAGGAAGGCACTCAAATTAATTTAGATTACTTAAAAGAACCTCAAAAAAGATTACTTAAACAATATGGTTTATTTGTCACAAAAACCTATAAAACAGTCATATTAGAAAAAATTAATAAAATATTAAGGTTCATAAAACAAACAAAAGGTGGAACTTCATATATAAGTAATGATGCTAATAAAACAGGTAGTGATTTATTAAATGATTTAAAAAATTTTGAAAACAACGGGACTTGA", "aa": "MNPSLISGLKPIIKKMNSDPTNPYSNVKQGLINILSNIDFNILARELNQTITTHYIDYKVVKFNPDLNKDEEKVFSVVLNAISPKDGIISFLRSMFGMPGSNRAFKENLIKMFNLSDKYKEIELPNSSVKIYVPDNDDTKVGFFDFLKIFASGLSNTEANEFNNYKIEHDFLALKSSIESLKEEGTQINLDYLKEPQKRLLKQYGLFVTKTYKTVILEKINKILRFIKQTKGGTSYISNDANKTGSDLLNDLKNFENNGT", "aa_hexdigest": "d5257342e868eee1ed4996e18030c368", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29618.561300000027, "isoelectric_point": 8.91064281463623 }, "id": "IGIGPGHMMC_1699", "locus": "GCA000148625_01697" }, { "type": "cds", "sequence": "contig_1", "start": 966968, "stop": 967471, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTTTAAGTCCAAAATCTCAATTAATTTATGATGTTTTAAATCATGGAAAAATTGATTATTTAGCTAATGCAATTGAAAAATATTTATTAAATTTTGATTTAGTTAAACAACAATTTATTACACCTGAGCAAGTTTATCAATTAACAAAAGTTATGAAAAAAGTGTTAAATGATAACAACTTTGCTTCAGTGTTTGCTACTGGCAAAATGAACAAGAAAATATTACCTAAATTAATGTTTGACTTTGTTTATGAGTTAAGTCATTATGAAGGTGGTGATGTTCTCAAATCACTATTCTTTAGCATTCTAGAACAAGCAAAAGCTAAAATAGCTTCTAAATCAACTTTAGTTGAACAAAAAGCTTATTTAGCAGCTGAAGTAAATAACTTATACAAATTACTACAAGATTTAGCTTCTATTGATTTAAACCAATACTTAAGTGCAGAATCATTAGTTAATGTTTCAAATGATCCAAAAATATTTATTAATGCAATTTGA", "aa": "MLFLSPKSQLIYDVLNHGKIDYLANAIEKYLLNFDLVKQQFITPEQVYQLTKVMKKVLNDNNFASVFATGKMNKKILPKLMFDFVYELSHYEGGDVLKSLFFSILEQAKAKIASKSTLVEQKAYLAAEVNNLYKLLQDLASIDLNQYLSAESLVNVSNDPKIFINAI", "aa_hexdigest": "ec3adc94fcbe2a614e966504f7d60dc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19022.938800000007, "isoelectric_point": 7.803462791442873 }, "id": "IGIGPGHMMC_1700", "locus": "GCA000148625_01698" }, { "type": "cds", "sequence": "contig_1", "start": 969026, "stop": 969718, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1", "UniRef:UniRef90_UPI000FF4EEE1" ], "nt": "ATGGTTAATGGTTTTAATGATTATAAACATGTATCAAAATTGCAAGATATCAATGTTGACTTAAATTTACCAACACAAGGTAGTGCATATAATCAAGGTTACTACATTAATGGTGAAAAATCAGATGATTTCAAAGGGACAAATAAAGACTATACACCAATTGAATATCAAATTGATTATCAAAGTGTAAAAGCTTCTTCATTGCCCGATTCAACTAAAAAAGAATATGAAGACTTTACAAGAAATGTTTTATTTTTTGGCAATGATAAAAGAGAATATTTGCCATTTAATAAAATAGGTAATATTGACCCTTCGGTAAATAGTTATTACTTTAAAAAAGATGACTTATTAAATCTTTACTCAATTTATAAAGCTGAACAAAACATTGAAAATAATAAAAACCCAGACATTGTTTTTGATTTAAAAGACATAAATAATTTAACTTTTGAACTAAAACAAAAAAACCGTTTCTTGAATATTTATGAAAAGAAAGGCGATACTTATGAACAAGTTTTTCTTGAAAAGAAACTTGAAAGAACTAACATTGTTCATTTCGATAAAACCTATAAAATAGGTAATTTAATGAATTTAACTATCAACAATAATGAAATTTATGCAACTCAATTAAGTTCATTATTTATTAATACTGACACAAAGGAAATAACAGCAGACTATTCAAAAGGTAAAAGTTGA", "aa": "MVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNSYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNIVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKS", "aa_hexdigest": "597a5b5683f5512ce0c003bbf20d6f55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 968726, "stop": 969844, "observations": { "mutation": [ 968827, 968866, 969028, 969841 ], "stop": [ 968827, 968866, 969028, 969841 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "query_cov": 1.0, "subject_cov": 0.49356223175965663, "identity": 0.991, "score": 456.0, "evalue": 8.05e-159, "gene_start": 1, "gene_end": 230, "reference_start": 43, "reference_end": 272, "reference_sequence": "MWRLFKEVFKSLSKNKVIVAGLSILVFLTSAIFTLLSNVSSSMVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNNYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNTVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKSWINQEKGIEIEPKQLAALFNFEPASFDGSTPNGLLFKQKDKTTNLDKFIKYNSSWAEINENLNLQSDWNLEDVFATNPKAIKAQKKLTLNKNNKFTLPIEWIAKEENIVRFLRWNYYTSFVDKDKSKDKWLGAFKTFMEDLIESSKTNPENKKMLDKLTTFSYWRKSKKNIVTPYKSDKTLDLSKKLQKNLLFH" }, "paralog": false, "identity": 0.967741935483871, "score": 713.0, "evalue": 1.81e-262, "description": "Internal stop codon at 968827, 968866, 969028, 969841. Nonsense mutation around 968827, 968866, 969028, 969841.", "psc": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF4EEE1", "UniRef:UniRef50_UPI000FF4EEE1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 26928.64820000004, "isoelectric_point": 5.372160148620606 }, "id": "IGIGPGHMMC_1701", "locus": "GCA000148625_01699" }, { "type": "cds", "sequence": "contig_1", "start": 970052, "stop": 971602, "strand": "-", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "nt": "ATGAAAAAAAATATGAGTATGATGGGTGCAATTGGAGATGAAAAGAATCCTGCAAGAAAAGTCAAATCAGATAAAACATTTAAAGATGTTGCAGGTAACAAAGAAGCAATTGAAGAAATTGAAGAAATTGTTGATTATCTTAAAAATCCTAAAAAATATGCACTTGCTGGTGCTAGAATGCCACATGGTATTTTACTTGGAGGCCCTCCAGGGACAGGTAAAACATTGTTAGCTAAAGCTACAGCAGGTGAAGCAAATGTACCTTTCTACTTTATTTCAGCTTCAAACTTTGTTGAAATGTATGTTGGTTTAGGTGCTAAAAGAGTTAGAACTGTTTTTGCAGAAGCTCGTAAAAATGCTCCAGCTATTATCTTTATTGATGAATTAGATGCTATTGGTCGTACCCGTGGAGCTGGTTTAGGCGGAGGTCATGATGAACGTGAACAAACACTTAACCAACTTCTTGTTGAAATGGATGGTATGACTCAAAATAATGGAATATTAATTTTTGCAGCAACTAACCGTACAGACGTTTTAGACCCTGCTTTAACTCGTCCAGGTCGTTTTGACAGAACAATTACAGTTGGATTACCTGATGTTAAAGAACGTGAAGAAATTTTAGAATTGCATGCTAAAGGTAAAAGATTATCACCTGAAATTAAACTTGATCAAGTTGCAAAAAGAACTCCAGGATTTTCTGGCGCTCAACTTGAAAATGTTATTAATGAAGCCTCATTATTAGCAGTTAGAGCTAACTCAGACGTTATTAGACGTGAAGATATTGATGAAGGTATCGATAGAGTTATGGCTGGACCTGCTAAGAAAAATAGAGTTATTACTAAAGAAGAACTTACAATGGTTGCTTACCACGAAGCCGGTCATGCTGTTGTTGGATTAAAAGTTAAAGGTGGAAACAAAGTTCAAAAAATTACCATTATTCCTCGTGGAAATGCCGGTGGTTACAACTTAATGACACCCGAAGAAGAAAAATATAATGCAACAAAACAAGAATTGATTGCAATGATTACTGGATTTATGGGTGGTCGTGCATCTGAAGAATTAATTTATGGAAAAGACAATGTTTCTACAGGCGCTAGTGATGATATTTCTAAAGCAACCAAAATTGCTCGTAGAATGGTAACTGAATTTGGTATGTCACAACTTGGCCCTATTCAATATGAAAAAGATGAAAGCTCTCCATTCTTAGGCCGTGATTATCTAAAAAGTGCTTCATTTAGTTCTCAAGTAGCCCACGAAATTGATCTTGAAGTTAGAAGAATAATGACAGAAGCAGAAAAGAATGCCGCTAAAATCATTAAAGAAAACAGACAACTTCTTGAATTAATCAAGACAGCTTTACTTGAAAATGAAACAATTGTTGCTGAAGAAATTGAATACATTGAAAAGAATATGAAACTTCCTCCAAAAAGAGAAGCTGACAAAACCAAAACCAAAGTTTATACTTTAGATGAGCTTATCAATGAACACAAAGATGACAAAAAATTAGAAGATTCAAAAGCTGAAACAAAAAAAGATAATTCTAAAAAATAA", "aa": "MKKNMSMMGAIGDEKNPARKVKSDKTFKDVAGNKEAIEEIEEIVDYLKNPKKYALAGARMPHGILLGGPPGTGKTLLAKATAGEANVPFYFISASNFVEMYVGLGAKRVRTVFAEARKNAPAIIFIDELDAIGRTRGAGLGGGHDEREQTLNQLLVEMDGMTQNNGILIFAATNRTDVLDPALTRPGRFDRTITVGLPDVKEREEILELHAKGKRLSPEIKLDQVAKRTPGFSGAQLENVINEASLLAVRANSDVIRREDIDEGIDRVMAGPAKKNRVITKEELTMVAYHEAGHAVVGLKVKGGNKVQKITIIPRGNAGGYNLMTPEEEKYNATKQELIAMITGFMGGRASEELIYGKDNVSTGASDDISKATKIARRMVTEFGMSQLGPIQYEKDESSPFLGRDYLKSASFSSQVAHEIDLEVRRIMTEAEKNAAKIIKENRQLLELIKTALLENETIVAEEIEYIEKNMKLPPKREADKTKTKVYTLDELINEHKDDKKLEDSKAETKKDNSKK", "aa_hexdigest": "62ae70dc926264f58314cc2e8230c3e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AS56", "query_cov": 0.9166666666666666, "subject_cov": 0.9594320486815415, "identity": 0.748, "score": 698.0, "evalue": 5.46e-249, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A449AS56", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AS56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "IGIGPGHMMC_1702", "locus": "GCA000148625_01700" }, { "type": "cds", "sequence": "contig_1", "start": 971837, "stop": 972145, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAAACCAAGCATACTGTCAATTATTTTAATTACAGTATTGTCATTCTTAGCTTTATCAATCGTTTCATATTATTTGTTTAAAAGATTTGGACCTAAGCCTCAAATTGTTGACTTAAATCAATTTATGAATGATTTAAAAGCTGCTTCAGCTAGTCAATCAGATGATGTTTATTTTGCAAGTATAGAGTTTGACTCGTATTATGGAACTGCAAAATATGTATATGCTGGTTTAGACTTAAAAGGTAACCGCATATTAGTTGAAAGAATTGCTCAAATTGGAAGATCTAATGCAGCATCATGA", "aa": "MQKKPSILSIILITVLSFLALSIVSYYLFKRFGPKPQIVDLNQFMNDLKAASASQSDDVYFASIEFDSYYGTAKYVYAGLDLKGNRILVERIAQIGRSNAAS", "aa_hexdigest": "e4fc9cd3f6dad3d53c507396bf652e80", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11379.032299999993, "isoelectric_point": 9.221702766418456 }, "id": "IGIGPGHMMC_1703", "locus": "GCA000148625_01701" }, { "type": "cds", "sequence": "contig_1", "start": 972729, "stop": 973094, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATTTTAGGAATTAGTGGCGGCCCAGATTCAATGCTACTTTTACATGAATATGCTTATAAAAAAGAGCCCATTTTTGTAGTGTGCATTAATTATAATCAAAGGATGGATTCACATAAAGATGTGGAAATTGTAGAAAATTATTGCAAACAAAATTTTATAGATTATCAAATATACAATTTTGACCAAAAAGACTATAAAACAGGCAATTTTGAAGAATGGGCCAGAGACAAAAGAATGGAAATCTTTATTCAAAATTATCATGAAAAGAATGCTTCTAAAATTTTGTTAGCACACCACAAAGATGATTTTTTAGAGTCATTTTTTATGCAAAAAGTAGCGAAAAGAAAACCTTCATTTTGA", "aa": "MYILGISGGPDSMLLLHEYAYKKEPIFVVCINYNQRMDSHKDVEIVENYCKQNFIDYQIYNFDQKDYKTGNFEEWARDKRMEIFIQNYHEKNASKILLAHHKDDFLESFFMQKVAKRKPSF", "aa_hexdigest": "515da2feb5bd3437c7b54be267890d98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14633.543999999987, "isoelectric_point": 6.506780052185058 }, "id": "IGIGPGHMMC_1704", "locus": "GCA000148625_01702" }, { "type": "cds", "sequence": "contig_1", "start": 973096, "stop": 973656, "strand": "-", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_013355065.1", "SO:0001217", "UniParc:UPI0001E330B0", "UniRef:UniRef100_UPI0001E330B0", "UniRef:UniRef50_Q6KHA3", "UniRef:UniRef90_C4XFM8" ], "nt": "ATGAAATTAATCGTAGGTTTAGGTAATCCAGGAAGCCAATATCGTTTTACAAAACACAATATTGGTTTTTTAACTGTCGACAAAATTTGTGCGAACTTTAATTTAACTCTTACAAAAGAAAAATTTAATGGCTTTTATGCAAAAACTGAAGATTTTATTTTAGCTAAGCCAACTACTTATATGAATAATTCTGGTGAATTTGTTCAAGCGATAGCTTCATTTTATAAAATAGATCCTTCAGATATTTTGATTATTCATGATGAAAAGGATTATGAAATTGGTCAAGCAGCAATTAAAGTAGGCGGTTCAAGTGCTGGACATAATGGTGTTCAAAGCATAATTAATGCTCTTAGAAGCAATGAATTTAAGAGGCTTAGAATAGGAATCGGAAGAGATCCTAAAATGGAATTAAAAGATTATGTTTTAAGTAATTTTACAAGTGATCAAATGTTAGTAGTCGAACAAGTTCTAAATGTTGCTGCACAAGCTGCAATATCTTTTGTTTACAATGATATTAAGATAGTAATGAATAACTTTAATGTTTTTCGTAAAGGTAAATAA", "aa": "MKLIVGLGNPGSQYRFTKHNIGFLTVDKICANFNLTLTKEKFNGFYAKTEDFILAKPTTYMNNSGEFVQAIASFYKIDPSDILIIHDEKDYEIGQAAIKVGGSSAGHNGVQSIINALRSNEFKRLRIGIGRDPKMELKDYVLSNFTSDQMLVVEQVLNVAAQAAISFVYNDIKIVMNNFNVFRKGK", "aa_hexdigest": "b4aa2dc89c41ca025eeb921623533bcb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B0", "ncbi_nrp_id": "WP_013355065.1", "uniref100_id": "UniRef100_UPI0001E330B0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B0", "RefSeq:WP_013355065.1", "UniRef:UniRef100_UPI0001E330B0" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330B0", "uniref90_id": "UniRef90_C4XFM8" }, "psc": { "uniref90_id": "UniRef90_C4XFM8", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q6KHA3", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHA3" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "IGIGPGHMMC_1705", "locus": "GCA000148625_01703" }, { "type": "cds", "sequence": "contig_1", "start": 973723, "stop": 973896, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64", "UniRef:UniRef50_A0AB32XD64", "UniRef:UniRef90_A0AB32XD64", "PFAM:PF13538.12" ], "nt": "TTGAATTATTATAAAGTTCAAGGTTCTGAATCTGATGTAGTTATCATTTACTTTGACAAAAACATAGCCAAATATATTACTAAAAATCACTTATATACTGCTCTTTCAAGAGCTAAAAAGAAAATCATTATTATGAATGATGTAGATGAATTTAAAGAACAGTACCTAAACTAA", "aa": "MNYYKVQGSESDVVIIYFDKNIAKYITKNHLYTALSRAKKKIIIMNDVDEFKEQYLN", "aa_hexdigest": "8a3a66c0030187053a56bd7e3f5b5554", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B1", "uniref100_id": "UniRef100_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD64", "uniref90_id": "UniRef90_A0AB32XD64" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD64", "uniref50_id": "UniRef50_A0AB32XD64" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD64" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13538.12", "name": "UvrD_C_2", "length": 45, "aa_cov": 0.7368421052631579, "hmm_cov": 1.0, "evalue": 8.881967473853347e-11, "score": 37.5797119140625, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 6808.7852, "isoelectric_point": 8.89220485687256 }, "id": "IGIGPGHMMC_1706", "locus": "GCA000148625_01704" }, { "type": "cds", "sequence": "contig_1", "start": 974178, "stop": 974558, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355067.1", "SO:0001217", "UniParc:UPI0001E330B2", "UniRef:UniRef100_C4XFM6", "UniRef:UniRef50_C4XFM6", "UniRef:UniRef90_C4XFM6" ], "nt": "TTGTGTTCAATTAATTACAATTTTGATGATAAAAAAAAGATCATTAATTATTATTCTGAAGATTCACAAGTATTAAAACTTAAAGGCTCATTCCGTGCGAACAAAAATATTGAAGAATTCTATGAAAGTATTTTAGAAAACAAACCATTTCACTTTGATGGTGAAAATGTAATTGCATTTAATCAAAATGATATGGCTAGAAAATACGAAGCTCTCAAATATTATTATAATTATATTGACTCGAAGTTTATCGATTCATCAAAAAATATTAAAGAATGTTTCCCAAATTATAAAGATGAACTTTTTGTTATTTATGCACTTCTAAAAGAATTTTATGGGTGCAATTATCCAGAAGGAAAACCAACTGAGTTGAAAAAGTAG", "aa": "MCSINYNFDDKKKIINYYSEDSQVLKLKGSFRANKNIEEFYESILENKPFHFDGENVIAFNQNDMARKYEALKYYYNYIDSKFIDSSKNIKECFPNYKDELFVIYALLKEFYGCNYPEGKPTELKK", "aa_hexdigest": "de79ea82f2bb0fc4db5e2b84f9c6e752", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B2", "ncbi_nrp_id": "WP_013355067.1", "uniref100_id": "UniRef100_C4XFM6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B2", "RefSeq:WP_013355067.1", "UniRef:UniRef100_C4XFM6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM6", "uniref90_id": "UniRef90_C4XFM6" }, "psc": { "uniref90_id": "UniRef90_C4XFM6", "uniref50_id": "UniRef50_C4XFM6" }, "pscc": { "uniref50_id": "UniRef50_C4XFM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15124.932399999989, "isoelectric_point": 5.89718189239502 }, "id": "IGIGPGHMMC_1707", "locus": "GCA000148625_01705" }, { "type": "cds", "sequence": "contig_1", "start": 974584, "stop": 974730, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5", "UniRef:UniRef90_C4XFM5" ], "nt": "ATGTTTATAACTAATACAAATGAACGAATGGTTTTAAACAAATTAAAAAATCACAACGTGAATGGTGTTTTAGCTGTTTTTGAATCTTTTGATATCAACAAAATGGTTTATAACAAAATAAAAGGTTATATTTCACATCAAATTTGA", "aa": "MFITNTNERMVLNKLKNHNVNGVLAVFESFDINKMVYNKIKGYISHQI", "aa_hexdigest": "e9c9e18933e5ad8ccf7977c8570a74e9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFM5", "query_cov": 1.0, "subject_cov": 0.8275862068965517, "identity": 1.0, "score": 98.2, "evalue": 3.61e-26, "valid": true, "uniref50_id": "UniRef50_C4XFM5" }, "pscc": { "uniref50_id": "UniRef50_C4XFM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.521000000001, "isoelectric_point": 9.60174388885498 }, "id": "IGIGPGHMMC_1708", "locus": "GCA000148625_01706" }, { "type": "cds", "sequence": "contig_1", "start": 974756, "stop": 974911, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAAAAAACTTTTATATACAGCAGTTTCAAGAGCTAAAGAAAAATTATTTATTTTAGGCGACTTAGCTTTATACGCTAAAAAAACAAAAACTGAAGATTATAGTAAAATAAAAACATATTTATTTGATTTAATTAAGGAACAAAATTAA", "aa": "MLNKKLLYTAVSRAKEKLFILGDLALYAKKTKTEDYSKIKTYLFDLIKEQN", "aa_hexdigest": "6a4f5b27229a6941212a3248b91eafb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6000.0543, "isoelectric_point": 9.60180835723877 }, "id": "IGIGPGHMMC_1709", "locus": "GCA000148625_01707" }, { "type": "cds", "sequence": "contig_1", "start": 975272, "stop": 975415, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATAAACTCAGTTGCATTATTAAACGAATTATCATACTTATATCAAACTTATAATCCAGAATACAATTCTGAAAAAATGGTAACCTTGATTCCACTTCATGATGGTCAAGTTGGAGCCAAAACAGTTAATAAATGA", "aa": "MKEINSVALLNELSYLYQTYNPEYNSEKMVTLIPLHDGQVGAKTVNK", "aa_hexdigest": "9eaa5eb767de2ec2b7712779c9ddf157", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.0606000000025, "isoelectric_point": 5.5737112045288075 }, "id": "IGIGPGHMMC_1710", "locus": "GCA000148625_01708" }, { "type": "cds", "sequence": "contig_1", "start": 975545, "stop": 976138, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGCAAAAATTATATTATGCAGAAAAAGAAGTTCCAACTATAGCACTTAGTTTTATTAAAGAACAAGAAGACTTTATTTTTGAAAAATTAATAACTATAAAAAATAAGATAAATAAAACTATTCCGCAAATAGAAGATGATACATTAACTGCTAAACAACTTGATGCTTTAAACATTGCACTAAATGAACCGATAAGCATAATTTCAGGATTCCCTGGCACTGGCAAAACATATTTAATTAGTAATATTTATGAGTCATTAATTGCCCAAAAAGTCTATAAAAAAGGTGAAATAGAAATTTTGACCCCCACTGGTAGAGCTGCAATTAATATTAAAAATAAGAGTAATAATTTGCCTGCAAAAACGATTCACAACTATTTAAAAATTGATAAAGAAGATGAAGACGTTGTTTATAACAATGATTGTGACAAAACGAAAGTTGTAATTATTGACGAGTTTTCAATGGTTAATTTAAAGATTTTTTATCAACTTTTAAGTCATTTATCCAAGCTTGAAAAGCTCATTATTGTTGGAGATAAAGATCAGCTTCCATGTATTGGACCTGGCAATTTAATGCACGACTTATGTAATTGA", "aa": "MQKLYYAEKEVPTIALSFIKEQEDFIFEKLITIKNKINKTIPQIEDDTLTAKQLDALNIALNEPISIISGFPGTGKTYLISNIYESLIAQKVYKKGEIEILTPTGRAAINIKNKSNNLPAKTIHNYLKIDKEDEDVVYNNDCDKTKVVIIDEFSMVNLKIFYQLLSHLSKLEKLIIVGDKDQLPCIGPGNLMHDLCN", "aa_hexdigest": "a5d67d5ae5ef378a748e56494718cd55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 55, "aa_cov": 0.25888324873096447, "hmm_cov": 1.0, "evalue": 1.2776328922275821e-11, "score": 40.78759002685547, "start": 49, "stop": 99 }, { "id": "PF13245.12", "name": "AAA_19", "length": 138, "aa_cov": 0.6903553299492385, "hmm_cov": 0.9710144927536232, "evalue": 4.34355586276608e-34, "score": 113.59687042236328, "start": 53, "stop": 188 }, { "id": "PF13604.12", "name": "AAA_30", "length": 151, "aa_cov": 0.751269035532995, "hmm_cov": 0.9271523178807947, "evalue": 7.130835936886575e-26, "score": 87.11857604980469, "start": 49, "stop": 196 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 72, "aa_cov": 0.36548223350253806, "hmm_cov": 0.9722222222222222, "evalue": 6.925773533079676e-14, "score": 48.144962310791016, "start": 49, "stop": 120 } ], "seq_stats": { "molecular_weight": 22330.68700000001, "isoelectric_point": 5.688355445861816 }, "id": "IGIGPGHMMC_1711", "locus": "GCA000148625_01709" }, { "type": "cds", "sequence": "contig_1", "start": 976238, "stop": 976828, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAGTGGGCCTAATAAATATAATAATTACATACTTGAAGGCTACAATATGATTGAAGCAACAAAAGAAGATTTAAAAGTTATGTTGAAGAATCATATTAGAGGTTTTAAAGAAGAAGATCATAATATTTTAAAAGAAAATTTAGGTGAAAATTATTTAGATGTTATTAAAGATACACCTAAAGAAGAAAGAAAAATATTTTACGAATTTATTAAACCCCCTTTGCTTAAAAAAATAGAAGCTTTTATTGAAGAAGTTCTAAACATGGGCAAAGATGAAAGATTTTTTATAACCAATAATATTTACAATTTTTATATTAAATTGAAGCAAAAATTACCTCTTGCAGATGGCGAATCATATTTAGATTTTTTTGACAAAAACAAAGAAAATCATGAAGATCCTTATCTCATTTTGTATATTGAATTAGGATTAAGTTTTGACTCAGTAGACAATTTTGCAAGCTTAATAGGATATTCAAAAGATTGCGAAACTAGATATTATGCAATAGTTTATAAATCTATTTATGATTTTGGAATTCAAGTTAATGATACATATTACAATTTGTCTGATTTACATAAAAATTGA", "aa": "MELSGPNKYNNYILEGYNMIEATKEDLKVMLKNHIRGFKEEDHNILKENLGENYLDVIKDTPKEERKIFYEFIKPPLLKKIEAFIEEVLNMGKDERFFITNNIYNFYIKLKQKLPLADGESYLDFFDKNKENHEDPYLILYIELGLSFDSVDNFASLIGYSKDCETRYYAIVYKSIYDFGIQVNDTYYNLSDLHKN", "aa_hexdigest": "9d758875175af236a0b93ed7cb931a44", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23331.19940000001, "isoelectric_point": 4.96354465484619 }, "id": "IGIGPGHMMC_1712", "locus": "GCA000148625_01710" }, { "type": "cds", "sequence": "contig_1", "start": 977209, "stop": 977415, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355069.1", "SO:0001217", "UniParc:UPI0001E330B4", "UniRef:UniRef100_UPI0001E330B4", "UniRef:UniRef50_UPI0001E330B4", "UniRef:UniRef90_UPI0001E330B4" ], "nt": "TTGATAATATCCTTATTTAAAGACAAAAAAATAGTTAATGAAAATGAATATCAAACTTATTTAAATGAGCTCAATAATGTTGTGTTTCAAATTTTATCAACTTTTATAGCTACAAATATGTTACACCACTATAATTCAGCAATTAGATTTAAATTTAGTTTCAAAAGAAAAGAAGATTCAATTTCTAAAAACTATATAAAAATTTAG", "aa": "MIISLFKDKKIVNENEYQTYLNELNNVVFQILSTFIATNMLHHYNSAIRFKFSFKRKEDSISKNYIKI", "aa_hexdigest": "c3d2c2de0067f47405f93b769c83b792", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B4", "ncbi_nrp_id": "WP_013355069.1", "uniref100_id": "UniRef100_UPI0001E330B4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B4", "RefSeq:WP_013355069.1", "UniRef:UniRef100_UPI0001E330B4" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E330B4", "uniref90_id": "UniRef90_UPI0001E330B4" }, "psc": { "uniref90_id": "UniRef90_UPI0001E330B4", "uniref50_id": "UniRef50_UPI0001E330B4" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E330B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E330B4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8193.411999999995, "isoelectric_point": 9.522318840026855 }, "id": "IGIGPGHMMC_1713", "locus": "GCA000148625_01711" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmopsis fermentans] [strain=JER] [gcode=11] [completeness=complete] [topology=circular]", "nt": "CTTTTAATTGTTGATAAGTTTGTGGATAACTTGTTTAAATAACTAAAAAACTTACTAATTATTTCTTACATTAAAATATAAATAATACTATTTTATATTATGGCAGAGAATAAAAATATAAAAGATAGTATCACAATGAAAGCAAATACATTAACATTTTTAAAATCTTTAAGTTCAGATGTTAATGACCAAATGTTGTTCAAAAATTTCATTTCAAACTTCACAATTGTAAGCATAGATCAGAACAATCATGTCAAAATTCAAACATGACTACAAAAACCAAGCTTAGAAATATTAATAAAAATGCTAGATGGTAATATTTTAAAAGCTTTAGAAGAAACTTTTGAAAGAGAATGCACATATGAATTTGTTCATATTCCTAAACCTATTGTTAAATTAACAAAAGCCTCAAGCAACAAAACAATTTCTTCTAAAACAATTGAAGAAGAAGTGAAATTAGGCGTTGATTTAAATAAAGAATTAACATTTGACAACTATGTTGAAAGTGATTTTAATAAAGATGCAATCAAATTAGCTAACTATTTAGTTAAAGGTGGAAAAGAATATAATCCTATTTTTATTTATGCTAAAAGTGGACTAGGAAAAACACACTTATTACATGCAATTGGTAACAAATTACAAGAGCAAGGCAAAGTTGTTAAATACATTAATCCAAGCATTTATGCTAGAGATATATCATTCTATTTGCAAGAAAATGATCAAGCAAAATTAAAGAAAATAAAAGATGAATTTGATAATGCTGATTACTTAATGTTTGATGATTTTCAAAGTTATGGAGTTGGAAATAAAAAAGCAACAATTCAAACAATTTATAATATTTTAGACTATCGTTTGAACACAAATAAAACAACCATATTTTGTTCGGATAAGCCTATAAATAACTTGAATGCGATGTTTGATGAAAGATTAATTTCTCGTCTTTATATGGGTTTACAACTTGAAATTAAAATTCCACAACAAAGTGATTTATTAAGAGTTTTAGATTACATGCTAGATATTAATGATATGAATCCAGAGGCTTGAGAAAAAGAAGCTAAAAACTTCGTTACAAGAAATTACTCAACATCAATTCGAACATTAATTGGAGCCATAACAAGATTAAAATTCTACAAAATGGAAATGGCCCGGACTAATTCTAAATATACTGTTGCTGTTGTTAATTCAATATTAAAAGACATGCAACAAACAAAAGAAAATATTACACCAGATTCAATAATAGAATATGTAGCAAAATATTACAAAATTCCTAAAAAAGAAATCTTAGGCAAAAGCAGACAAAAAGATGTCGTTCTAGCAAGACACATAGCTATTTATGTCATAAGAACACAACTGGAAGTTCCATTAGAACAAATTGGTCATATATTTGGTAATAGAGATCACTCAACTATTATTAATGCAATTAGAAAAATAGAAACTGAAAGTGATATGCCAGATAAAACAATTAAAAGAACCATATCAATGATTAGTGATGATATCTACAAATTAAAATAGTTATTAACTACATATAAACATTAAAATTAATCAAAAATTTAAAAAATAAGCAAAAAAAACATAAAAAATAAACTTTTTAGAAGTTATCCACAAAATAACAATGCTTATTAATAATATATAAGGAGTTATTATGAAATTACACATTAAAAAACAAATTTTAGATGAAACATTGGAAATTGTTTCAAAGTATACAGATCCTATTAATTCATTGTATGGGTTCAGATGTATATTAATTAAAGTAGATAATGAAAAAGTAACATTTTCAGCATCTAATGGAGTTATAAGTATAGTTAAATCACTAGATGTTGAAGATGTGGATATTAAAGTAGAAGAAACAGGAACATTTTTAATTCAAGCAAATATATTTAAAGCTATTATTAAAAAATTAAGTGAAACAATTACTTTAAGTCAAGAATATTCAACAACATTAGAAATATCACAAGGCAATTCAAATTATTCATTAACTACAAACCCAATCAATTCATTTCCACAAATAGAAGAAAATATTAATTTAAAAAGAATTGAAATCGATACAAATGAATTTAAAAAAGCTATTAGATGCACAGCTTTTGCTGTTAGTCAAGACGCTTCATTAGTATATAAATGTATTAATTTTAAATTTAAAAATAATAAATTAAATCTAACAGCTACAGATGCAGCAAGACTTGCATATTACACAATGGATCTTAAAAGTTCGCAAGTAGATAATGAAGAATTTTCAGTAAACAATAAAGATGTTAAAGATTTAATACCTACAGATGCATCTAAAAAAGTAACATTATTCTTTAATAGAATAAAAATAGGAATTGAATATAAAAATACAGTTATCACTTCAAGAATAGTTGATTTGCCTTACCCAGATGTTGAAAGTTTATTTGCTTCAATAGATATTGAAAATAAAATATTTATTAAAAAAGAAGAAATAAATGATTTAATTAACAAAGTTTGATTAGGAACAAGTGATAAACAAAATAGATTAGAATTTACAATTAATAAAAATGAAATGACTGTTTTAAATAGATTGAATGAAATCGGAACATCTATTGCAAAAACAGAAAATTTTAAATTTGAAGGAAAAGCTTTTGAATTTGATATTAACTATCATTTCTTAAAAGATGCTTTAAGTGTGTTTGAAGGTGACGTTTTAATTCTATTTGATAAAAGTGTACAAAAAATTTTAGTAGTTTCAGATTCAAATAAAGAATCTAAACAACTAGTAACTCCTATGAGAAGATAATATGAATAATGAAATTTATTTAAAAGATGATGTAATTACACTAGGTCAATTATTAAAGAAAATAGGACTTATTGACACAGGCGGATCAGCTAAAGGTTATTTACTTTCTAATAAAATTTTAATAAATAATAGAACACCAAATGGACGTAGTGCAAAAATTAAAATTGGTGACATTGTGTGGATAAATAATAAAGTTTTTATGATTAAAAAACTTGAAAAATAATTAGATAATAGATATTTAATATCTTTTATTTTTTTATATATATTAAATCATTTAATAAATACTAAAATGTTTGTTTTATAGTTATTAAAGAAAAAATAAAAAGCGAGAAAATCTCGCTTAATATAATAATAAATTAATTAAATGTTACCTAATATTCATTTAAATACTTTATATTCATCATCGTCAACTGATGTTTTATATTCAAATTTAGTTAAATCAAATTTAACATGATCTCTAGCAACAATTCTAAATGATGCAGCTAATTGTGTATTACCATTTATTCTAAAAACTTTTATGTGGCGAGACATCGGTTGATCTTTATTATATTTATTACCAACAATATGATGTCTAATTAAAGTATCAAATTTTTCATCTTGAACTGTTTCAGTATCAGCTAAAACAAAGTTAAATACATATTCAAAATTGAAGTTGTTTTCCATTGTCATTTGGTTAAAACCATATTTTGTTAAGCCTTGTTTAATAGATGTTGTCATGCCAAATGGACCTGCAACATTCATTTCATTAATTGTGTTTTGATCTACTTGTTTGAACAAAATATTATTTTTTGCCAAGTAAACAATTAACTTCATTTTGTCTTTAAATATCATATTTTCAGTTGAAGATTTAACGTTTTTGTTAAAGCCACTGACTGAAATTGTTTTAGTATTTGAATTTGAATCAAACGAAATGTTTAATTCAATGTCTAATTTACCTTCAGCAACATTAGGTGTTATTTTATTAATAACAACATCAACTGTTGAATTTATTTTTTTGAGATCATCTAAGTTTGTAATAGATACTTCATTTTTTGTAATATATGAAGACAAGAAGTATTTTTTGTCTAATTGAACATTTTCTTTAATTAATTGGATATTTAATAGCTTAACAATTTTTTCAGCTGTTAAAGTTTCTTTCTTGAAATCATTAATGATGTATTCTTTATTAATAACTTCAGTTTCTTTTGTGAATTTTAAGTTGAATTTAATAATACCTTCTAAATTTTTAACTTCAACAATAGTTGTTTCAAGAGAAACACCTTTAGTTGCAAAATCATAAGCAGTACTTGTTCTTTTGTCTGTGATTGTAATGTTGTTTACATCTGTGAATTCTTTAGCTTCTTTAGCTTTAAATGAATCAGCAATTGTAACTTCCATGTTAGTGATTTCTTCTGCTAATTCGCTACGGTAGAAACCTGAAATTGTAATTGGAACTTTATTAGAGAAAACATCTGGATGTTGAGAATTACTTAATTGAACATTAAATGTAATTTTGTCATTTAAAGTTGTGAAGTCTGAAATTTTAACGCTGTAACCTGAGCCAAAATCTGGGCTAACAATGTTTTCTTGAATGGCATTTTTAGCTTTTGTTGTTGTTTTGTTTTTATAATCAAATTCATTAATTTTTGAATTTACCATTGCTGTCAATTCAGCAGGTGTTTGGATTTTAGCTATAGTAGTGCTACTTTCTTCATAGTGTCTTACATATTTGCCATATACTTTTCCACCTAAGTAGAAACTTAAATAATAGTTTTCATTATCTTTATTTAGTAATACTTTTTTAGCTGAAGTATCATTATCTTCAGAAAATAGATATGCAGTAAAATTGTAACTAAATAATTCAACCCCATCTATTGAAATAGAATTGTTAAATGCATCTAAAGTTGTACCATCAACTAAGCTTTCTAATTTATTTAAAGCTTCAGTTGTTTCAACAAGTTTAATTTTATTAAGTTTTTTTAATGATGTAATTATTTCATTCGTTGTTGGTCTTACTTGAAGTTCTTGTTCACTTCTGAAGAATTTGTTTCCTTTATAAACAAAACTAAAATCAATTTTTCTTTTACCACTTTTGTATTCAGTAGAGCTAGGGCTAGAATTTGTGAATTTAAAAACAAAATCTGAAAAATCTGATGGTGTTTTAAGTCCTTCTATTTTAATTTTGCTTTCAAAAATATCAAAAGCATTTACTTTGTTAGTACCGCCTTCATATGATAATTTAGCTTTTTCAAATTCTTGAACTAAAGCATCATAATGATCACTTTTGTGATATATGTATTCAGTTCTAGTATTAGATTTATTGTTTTCATTGTGAATATTTGTAAGATGAAATTTAATTTCTAACTGATCAGCATTTTCTTTATATACTTCAACAGATTCAAAAACAATTTTTACTGTTGATGGTAAGTTATTAATAAATATTGTTTTGTATGTTGTTGTATTTGGATTTAAAAGTTCAGTTTTTGTTTCTTTTAAAGTTTTAGATCCATCTGAATAAATTTCAATATTTCCGAGTTGATAATTTAAATATTTGTCTGTTAATTTAAAACCATCAATTTGTATAGTTCTTTTATTTGTAGAAACTTCATTTCTAATTTTGTTTTTAATTTTAAATTCAAATTTTAATGTTGAATAAAATTTACTCAAAAGTTTAGATTCAACTATTTCAAAGGTGTTTTGGTCAAAATTAGAGTAAACAATTTTATTTATATTAGCATCTGTAACAAACACATTTAAGTGATCTGGATATTCAGCTTTAATTTGATTCAATACCATATCAGGTGTTAATAAAGTTTTTATTTCAAATGTTTTTTCTTTAGAGAATTTATTTGAATCTTTTTTATCAACAACTTTAATTTTTAAAATAACTCCTGTTTTATCGGTATTTTCTTCTAATGATACTACTTCACAATTTACTCTAGCATCAATGCTAGCAAATTCGAAGGCATCTTTTCCTTGTGAAACCACTTCGCTACTATATAAATTGTTTTTTAAAGTAACTTGTACTTTTTCTAAAAGATCATCTAAGTTTTCTTTAGCCTCTAATTTAAATGTAAAAGTTTTTTCTTTAGACATTTTGTCTTTGTTTTGTTTATCAACAACTTTAAGTGTTAATTCAACTTCACCTTTATTTGCACCTTTTTTAATTGATACAATTTTAAAATTAACTTTAGTATCTACATCTGTAAATTTAAAAATGTCTTTGCCTTTAGCTATAACTTCATCAGCAGTTAAATTTTTATCTAATAAAGTTACTTTGATTTTTTCTAATAAACTATTTAGTTCTGTTTCAGTAAGAGATTTTGGAGTTTCCTTAGGATCTTTATGACATGAAATAGTAGTCAACGGTAAAGTTGTTACTGTTGCAATTGCAGGAACAATAGCCAAACTTATTTTCTTTATTTTTTTCATATTTTTCCTTTCGTTTTTTTATTTTGTCAGCCCTTTAATTATACAAAATTGACTAAAAATGCAAATTTTCTATTTTTCTATAAAAATAGATTAACAAATAAAAAACTATTTTTATTAAGGTGAATATTCAGATTATCAAAGCGTTTTATTTGTTTTTTGAGAACTATAAATTTTTCCATTTTGAGTTTCTAGTCTATATTTAATAATTACAAATTTGTGCATTTGTAATTCATTTTTTTCTTTGTTTTGTGAATTTAAAAGCACTTAGTAGTATTTAAACTATTATTTAAAATTTTAATAATGCCATCAATTTTTCCTTTGTCAAATGTTATAGCATTACTAATTTCTAAAGAAGCTAATTATTATTTTTAAATTTAGATTCATAATCAAATTTTTAGTTTTCTAAAGATCTTTCTATGTCTTCATTTGACTTTTGAAATTTGCCTAAATGTTTATAAGTTTTAGTATTTATTTTTTTATTTTGGTGCCACCAGATATATTGTAAAGTAATTCTCAACAGAATCTTTGTAAATCATTTTTAATTTCCAAATCGACTGTTTTATAGTATTTTAGTTAAAATCATAAGGCAAAATAATTTTAACATTCTGTAGTTAATTTTTCAACATAGTCTTTTCAAAATTGAAATTTTTAACTTCAAATTTAAGTTTACTATTTAATTCATTTAGTTTTTCTTTGATTTGATCATCTGTGTAATAGTCAGGTAATTTATCTTTGGTGTTTTTTTAAAACCACCATTGTATACTTTTGTAAATTTCATGACTTGATCTAAAATTAGAGTGAGAATTGATACCTCAAAAATACTAATTAATTTTTACAATAACATTTGTACTATCATTGTTTCCAATTTAAAACCTTGAAAATAATCTTTTAAAATTTCTCCGAAACTGCCAATTCTAAGTTATCTTTAATGAATTGTTCTTTTTTATAATTTGTGATTTTTTTGTATTTTTTAATAGTCATATTTTTTAATGATACTTTTTCGCCATCACTATAAACATATTTAAGAGAAAAAACAATATAAACAAAATTTGCTTTTTCATAATATTGGACGCTTTGTGTATAACTTGTTTGACATAGCATATGAATCAGGAATGATTTATAAATTTATCTAGATAAGGATTATTATCAAGTTTAAATTTAATTTTTCTATTGCTACTTCATTTACATATTTATCTTTGATTGCTTTGTTATCAGCTAGATAAAGTATTATTTTGATTTGACTTGCTATTTTAATAATTTTATTATTAGTTAAATTAAAAATTAATTCAAAACAAAAAGAGCCTATAAATAAGCTCTTAATGTGCTAATTTTTGTATTTGTTTTTACCCACTTGAACTTCAACATTTGTATCTATTTCAATTTTTCTTGTTTTAATATTGCTTGCATTTAAAATGTGCCTTGCTATTTCTGCGTCTTCAGTGTCATGATATTTGTCATCTTCATAAACTACTTCACTAATGCCTGATTGAGCTATGGTCTTAGCACAACTTGAGCATGGATATAAAGAAGTGAAGATTGTGCAACCTTGAAGATTATTGTTTGTATTTAGAATTGCATTCATTTCGGCATGAACAACATAAGCATATTTAGTTTCTTTTGGACTTTCACCTTCACGGTCTCAAGGGAATTCGTCATCTATGCCTTTTGGCATTCCATTGTAACCTAGAGCAATAATTCTTTTGTTTTCATTAACTACGCAAGCACCGACCTTTGTTGAAGGATCTTTAGATCTTAATGCAGATACTTTAGCTAAAGCCATAAAATATCCGTCTCAATATAATGATTTGTTTTTCATGCAAGAATTATACTTTTAAAAGTAAAAAAGCAAAATTTACTGCAAAAATGGAAAAATAAAACTTAAATCGTGAAATTTAAGCATTATAATAAATCTTTAATTTTGTCCATTTTTTCTCAGAATATTTGCTTTGTTTCTTTCGGGCATTCAACAGCAACATTTGAAAAAGCTTCAGGACCTGTGTGAATTGCAACAACAGGAGCAAGTCAGTTAACTCAAGGGTCTTGGTGGTATTGTTTTTTGAATTCTTCAATAAAAGTTTTTTGTTCTTCTTCTGAAGCACCTGTGTGTATATAAGCAGTAAGAAGTACATTTTTCTTGTCATTGATTTTGAAGTTTTTAGCTTTAGATACTATATTTTTAATAGTGGTTTTTGAAAAAACATGACCGACGCCTTCTTTTTTAAGTTGACCACCATCAACAGCAATTAAAGGAACAATTTTAAATAATTTTGCTATGGCTGCGGCAGCAGGATGCAGTCTTCCACCTTTAACTAAATAATCATTGTATTTAGGTATAAGGGTAATTGATTTGTGGAACCCCCCGTCTTCAATGTATTTAAGATATTCATCCATTTTTGAAGGATCTTTAGCCATTTGTTGTTCGAATCAAATTAAATCTAAAACAATTAATTGAACAATTTGAACTGATTTAATTGTTCTTAATTTTGGAAACTCTTCTTCCATTGTTTTTGAAGTTTGATAAGTACTTGACAAATGATGAGAGATTGGATAGAAAATAATTTTGTCATACTCTTTTGACAATTTTTCAAATAATTCTGTAGTTTTAGCTATATTGATAGCCGATGTTTTTACTTTAGCTTTTTTAGTGTAAAAGTTAAACAAATTAGAGGTGTTGATATCAACACCATCTTCAAATTCCTTCTCATCGATATTTATATGTAGAGGGGTATAAAATCATCCTAGTGCTTCAGCTTGTTTTTTTGTTAAAGCACATGATGAGTCGACAATTATTGCATATTTCATATTTTATATTATATAATAAAAATATTATGGGTATATTTTTTAATTTAAATTCAAAATTTAAGAACACAAAAATCATATATTTTGATACAACAATAAACAAAAAAACTATGGATATTTATGAAGATATGGTTGTTTTTAGAGATGAAAATTTTAATGTAAATTCGGTTAATTTACTGAATGCAAAAAGTAAAAAAAGAGATGAAAAAATATTTGGTTTTTTAAATGAAAATGAAACTAAAAAACTAGTCAATAAATTGCACAAATATAACAAAAAATATTTACTAAAATCAATTAAATACTTTAGAATTGGTAAAATTGTAGAAAGAAAAAAACACCCAAAAAGCGATAGATTATTTGTATTAAAAATAGATTTTGGAAATGAAGTAAAACAAATAATAACTAACACAACATATACACTTGAAAATAAATATTTTACATTCTGTTTACCAGGATCAATTACAGCTAATGGATTAGAAATTTTAGATGGTAAAATTATGAATGTAGAAAGTGATGGAATGCTTTGCTCAGCAGAATCTTTAGGTTTAGAAGCAAAAAGCTATAAAGAATTTGAAGAAGGCTTTTTCAATAATGTTTTAGAAGAAGAAAAACAAAAATATTTAGGCAAAGAAATACAAGAATTATTGCCAGAATTAATAAGCAAATAGGAGTAATCATGACAATATTAGAAGATTTAAAATTAAGAGGAATATTAAAGCAAATTAGTAATGAAGATAAGTTTAAAAAACTTGATCCTAAGAAAGTTGCTGTATATTGTGGTTTTGATCCAACTGCGCAAAGTTTGCATTTAGGAAACTATGTTTTAATTTCAGTTTTAAAAAGATTTAAGCAATATGGTTTTAAAGTTTATGGACTTATTGGAGGGGCGACAGGAATGATTGGCGACCCTTCATTTAAAGATAGTGAAAGAGTTCTTTTAGATAATAAAACTTTGAAATTAAATAAAAGTAAAATTAGAAAACAATTAGAAGCTCAAGGCTTAGAAGTAATTGACAACATTCAATTTTATGAAAAAATGAATGTGCTTGAATTCTTAAGAGATGCCGGGAAACTTGTTAATGTTTCATATATGATGACTAAGGATTCAGTTCAAAAAAGAATTGAAAGAGGTTTGTCATTTACTGAATTTTCATATCAAGTTTTACAAGGTTGAGACTTCTTGCAACTTTATAAAAATTACAATATAATGATTCAAGTTGGTGGTTCAGATCAATGAGGTAATTTAACTACAGGTCTTGAAATGATTTCTAAAGTTGAGGGTGATGATCATAAAGCTGTTGTAGTTACTGCAGACTTATTAACTGATGAAAATGGAAATAAAATTGGTAAATCAACAGGTGGTGGCAAACTTTGATTAGATAAAACTATGGCTTCACCATATGAAATGTATCAATACTTATTAAATCAACCAGATAGCAAAGTTGAAACATTTTTAAATTGATTAACATTTTTAGATAATAAAAAAATAGCAAAAATTATGGAAGAACATAATCAAGCACCGTTTAAACATGTAGCTCAAAGAGTAGTAGCTAGTGAAGTAGTTAGAGATATTTTTGGTCAAGATGAATTAGATTCAGCTATTCAAATTACAGAAGTTTTGTTTAACAAAAATTTTGATACAAGCAAACTAAATTTAAAAGATCTTAAATCAATTGAGCAATATTTACCAGTAGTTGAAATTAAGCAAAATCAAAATATTATTGAAACTTTAATTGAAAAAGGTTTTATTGCTTCAAAACGTGAAGCTAGAGAATTTATGGCAACTAAAGCTTTAAAAATAGATGAAGATATAGTTGAAGAAAACTCAGTTTATAAACCAAAAAACTTTTCTAAAAAGTATGCATTCTTTAGAAAAGGAAAGAAACAAACTATACTATTAAAAACTATTTAGGTAATTAAAAAAGTGGAAAAGACCGCTTTTTTAATTTTGATTACATAAATGTTTCAACTAAAGGAATAATGCCAACTAGTAAACCTGCTGCAAAAGGTGTAGCACCAGGAACAATTGAACCATATACTCAATTTGTTGAAGGAGCTTTAACCACTTCTCCTGTTTTTTCATCTTTTATTCCATCTTTAAAGAAGAATAATACTGTCATTTGGTAAACAAGTCTTGGACTTCAGTCTCTTGCTGGATTAAGTGCAAAACCTGTTACTCCACCAAGTGACATACCAATAGACATAATAACTAATGCTACAGGTAGAGGACCTAAACCAGTAAATCCTGATTTGTTTATTAAGTAAATCATAAGAATTAAAACAGCTGTACCAACAAATTCATAAAGAACATTTTGCACTCAAGCATTTTCATGTGAAGCGCCTGTGGCACTTGAAGCTTTAAGAAGGCTTAAGTCATTTTCTTTAATGTGTTTTCAGTTCATTACATTTAATAAAATTTGACCAAGTAAGGCACCAACAAATTGCATTATGATCATTAAAATGCCATTGCCTCATGTTTCGCCTGTTGTATGTTTTCCAAATGAAATTAATCAATATAATGTTACTGCAGGATTCAAGTGACCTAGTCCACCAATTCCAATAGTAGCTAAAGCACCAACAAAAATTGCTAAACCTCAACCTATTGCAATTAATAATCAACCTGCACCTTTACTATGCATATTTTTAAAATTGTTTGCTGCACAAACGCCGTTACCTAGTAGTATTAATAAGAAAGTACCAACTAGTTCTCCAACAATTAATTGTCAACTCATAATATCTCCATTTTTTAATTTAGTGTGATTCTTTGCACAAAAAAATTATATTGCTTAAATTAAAAAAGTTATCAATATGAAAATATGAATAAAGGCATTATTTACTATTATTTTGTATAATTTATAATAATTTATATGTGCCAATTTCGCTTAGTGGTAAAGCAGCTGACTCGTAATTAGCCGCCATGAGTTCGATTCTCATAATTGGCACCAGATCATTTTAAGTAGCTCAAAAAGGCTACTTTTTTGATAAGCATTTTTTTAAAAACAACTGTTTTATAGAATTATTAGTCAAAAATGTATAAAAAAGATTATTCTATTTTTGTAGATTAAGTCTTAATAGTTTTAAATTGTTTCATTAATTTTTGGGAATTTGTTTTTGTTGTTTTCAAATAAAGATTTTTTCAATTTAATATTATTTCATGTGTAAATAAAACTACATAAAACAATAGATATTAATGCAAAAATAGTCATGGTTATTAAATACTCAGAATATTCATTTGAAGTTCTTTTAGGTTTTAATGTAAATGCTACAAATAATATTGTGAAGAGAATTGTTAAATCAAATTGTACTGTTGTTGTAAAGCAGTTGATTTGTATTCTTTTTTCATAATACTTTTAATAAATAAATTGAATAATCTAAAGCTGCAATAAGCATAATATCTATAACAAATTTAAAAATCAAACCTAATGAAGCTCCCATAAATATTATGTCAATATTTAAGTTTTCTATTCTACCCGCCAAGAAAAACAAAATTAAATGAAAAAATAATTGCTAAAATACCTGAAATTATTCTTTCATCTTGATATTTGTTAGCGAATTTTGTTATTCTAGTTGTTGTATTTGTTTTTGGATCTCAATGATCAACGGTTTTTATAAACATAGGAATAAATTTACACATATTGATAAAAATATAATAGTTAATATACTTAGCAATGCTATCGATATAATTCCTCGAACCAAAACAGTTTTTGAATTATAAAGAAGAGTTTTTTTCTTTTAATTAAGTGTTATTCATTTGCTTATTATTATCCCTTTTTGACTTTTGAATGCAAAAAATGTTTCAACTATACCTACATATCTAAAAAAATGTTTGAAAATGGAGCACTCATAAAAATAAAAAATTTTGGCTAAAAATATTTTTATATAAAATATAATAATTAAGAAAAAGAGGATTAATGAAAAAACATATCGGATTAAGAAAAGAAGCTAAATATGATGCAGATTTTGATGATAAAAAAATTAATGTTTATATTGACTATTTAGTTTCTTGAATGAAAGAAAGAGTTGCTGATGCTTGTGCTAAAGGAATTGTTTTAGGTATTAGTGGAGGTATAGATTCTGCTGTAGTTGTCGCCTTAGCAAAAAAAGCTTTTGGCAAAAATGTTCTTGGTGTAGTAATGCCAGTTGATAGTATGCAAAATGATTTAAAAGATATCAAAGAATTAGAAAAAGCGGTTGATTTAAAATTTATAACTATTGATCTAAAAAATACCTATGATACTATAAAACAGGCTTTACCGTCAATTAACAACAATTTAGCTTTAAGTAATATTAAACCAAGATTGCGTATGGCTGTCCTATATGCATTAGCTCAAGAAAACAATTATTTAGTAACAGGAACAGGCAATAAATGCGAAACTCACATTGGTTATTTTACAAAATATGGCGATGGTGGAAGTGACATTTTGCCTTTAAGCAAACTGTTAAAAAGTGAAGTAAAAATTATTGCAAAGCACTTAAAAGTGCCAGACTCAATTATTAATAAAAAACCTTCTGCCGGTCTTTGAGAAGGACAAAATGATGAAGATGAATTAGGATTCAGTTATAAAGATTTGGATTCTTATTTAATGGATTCTAATGCAAAAATTAGTCGAGAGGTCAAAAATAAAATAGACAAAATGTACAAAAATAGTGAACACAAAAGAAATGTTTCATTACAACCTCAAGAAATTGATACAATATTAAAATAAAAAAATCACAAGGAGATTTATTATGTCAGGACACTCACACTGAGCAGGAATTAAACACAAGAAGGGAGCAAATGATGCTGCCCGTGGTAAGATATTCCAAAAAATGTTTAAAGAAATTTATGTAGCAGCAACAACAGCTGGCGGCCCAGATCCAGATATGAATCCAGCACTTAAATTAGCTATTGCAAAAGCTAAAAGTAAAAACATGCCTAAAGCAAATATTGAAAGAGCTTTAGCAAAAGTAAAAGGTGATGGTAAAGATGCATCAGCTTATACAGAAGTTTTATTCAATGCAACAATTGCAGGGGGAGCTACATTTTTAGTTGTTGCCTTAACAGATAATATGAATAGAACAACAAGTTCTATGAAAGCACACTTTAATAGACAAAATGCAGCTTTAGGTAAAACAGGTCAAGTACCTTTTGCTTTCGATCATAAAGGTGTTTTAGAAGTTTCAAAAAACTTAGTTGGAGAAGACGATCTAACTATGGTTGCACTTGAAAATGGTGCCGAAGATTTTGAAGTAACTGAAGATTCTTATATAATTACAACAGCACCAGAAAACTTTTCAAAATGTAAAAGCGCTGTTGAAAGTAACTTAAATATTAGTGATTTTATGCAATGTGAAGTTACATATATTCCTAACACAACAGTTGCTTTTGATAAAGAAAAAAGTGAAAAACTTCAAGAATTTATCGCTCGTTTAGAAGATGACGATGATGTTCAAGAAGTATTTCACAATATTGAATTTGAAGAATAATTATTATCTTTAACTTCAAGTAGAAAGTTAGTATGAGAAAATTTATTAGAGCTCTTGAAAATGGTGCTTTAACAATAGGCACAAGAGAAATAGCAGCTAAATTCTATTTGATCTTAATTGCATTTGCAATTATATTTTTAGTGCCAGTTTTAGTGGCATTAATTTTTCCTTTATTTAAGAAAAGCTTAAGTAAAAAAGGAAGTATATTGCTTTATGCTTTTGTAACTGGTTTCTTTTTAACGATGGCTTTATTTGGATTTTTAAAAGAAGCTTTGGAAATAAGTTCAATTAGTGCTCCGGCATCAATGCCACCAGAAGCTTCGTCAGCGCAGATTAAATGAGCAACATATGGTTGAAACATATTCTTATTAGTTTCAGGTTTAGTTGGTGGATTATTATTTGCACTTGGAATTAGAATGACAGTTAAAAAGATAAGTGAAAAACATTTAGCTAAAAGTAAAGCAAGTGTATTTGTGCATACACATGATATGGTTCATAACCATGAACATGGTGAAAATGAAGAACATTCTCATGATCATGTGGCACAAGAACATAAAATATCAACAGACGAAAAAAATGCTAATAACAAACTAAAAGTAGTTGCATTATTGTTGTTATTAACTCACAGAATACCAGAAGGTTTCTTAATTGGTTATTCATTAAATAACTTATATAATCAGAATATTGGCTCATTAAGTATTGCATTCTTTGTTTCATTTATAATGCACTTAATACCTGAAGAAATGGTTTTTTATTATAGACAACGTGAAATGGGTGTAGGTCGTTGAAAAGCAATTGGAATATCAATAGGTTGTTTATTCTTATTCTTGCCTATGATGCTTTTAGGAATATTCACAGGGGATTATATTCAACAAATTTGACAACTAAGAGCATTCTTACAAGCAATGATAGCTGGTATATTCTTCTTTACAGCTGTGATGGAATTTCTACCTGAGTTTTATCATGCACATCATGACAAAGATTTGTTCAAAAAAGTTATGATTACATTTATGGCTGGACTTGTGGTTTGTGCCTTGATATTAGCATTGCATCAACATGGGACATATAATTAATTTAAGTACTTAGCGAAGTACTTTTATTTTTATTATTTTGCTGTTTTATAGTGTTTTTGGTAATTTTTCTGATACTATTTTTTCATTTTTCCCTCGAATAAAATTGTTTAAGATATGAAAAAAATAATTTAAAAAAATGAACTCTATTGAGTCCATTTTGTGGTCTAAATTATTTAATAAGACCTTCTCATTTTCAGTTTTTAACTTCAGGAATATCTGTACCAACTTCACGAATGTATTTGTTGTGGAATTGGATTGTTTCTTCCATTTTGTCCATGAATTCTTTTGCTTTTGATGATCCGAATACAGCTGCAGCAGCATCGCTAGCCATGTGGAATCTGTCCATGTGACTTAATTGACGAATGTCGAATGATGTTGTAATGTCACCATTTTCTCTATAACCGTGAGCAATTAAATTGTGGTTGTGACGGTCAAAGAAGATGTCTCTTAAAATACCTTCAAAACCATGGAAAGCAAATAATACTGGTTTGTCTTTTGTAAAGATTTCATCAAATTCTTCATCACTTAAACCGCGTGGGTCAATTTTTGGATGTCTTAATTTAAGTAAATCTACAACGTTAACAAATCTAATTTTCATACTTGGATATTCTTTGTTAAGAATTGAGATAGTAGCTAAAGCTTCTAAGTTAGGTTCTGTACCACTAGCAACAACAACAAGATCTGGAGTTTCATTTCTTGAACATGTTGAAGCTCAATCAATAATGTCTAAACCTTTTTCAACCAATGTTTCAGCTTCTTCTACTGAGTAGAATTGGTGTCTTGGTTGTTTTGAAGAAACGATTAAGTTAATTACATTTCTTTCTGTAAATGCTTTATCCATAACGGCTAATAATGTGTTTGTATCAGCTGGTAAGTATTCTCTAATTAGTTCTGGTCTTTTATCTGCCATGTGTCCAATAATACCTGGGTCTTGGTGTGTATAACCATTGTGGTCTTGTTGGAAAGCAGTTGAAGTAGCCATAATGTTTAATGATGGATAATCTTTTCTTCAGTTAATTTCTTTTGCTTTTCTTAATCATTTCATGTGTTGTGTAACCATTGAGTCAACAACTCTTAAGAATGATTCATATGAAGCAAAGATACCATGACGACCTGTTAATACATAACCTTCTAAGAAACCTTGAGCTTGGTGTTCTGAAAGTTGTGAGTCAATGATTCTACCTACTGGACTAAGAGCTTCATCTAATTTAGAATCAATTCTTTCCATTCATTGACGGTTTGTAACTTTGAATAAGTTGTAAAGACGGTTTGATTTAGTTTCATCTGGTCCAAAAATTCTAAAGTTTTCTGGATTTCTTTTTACTAAGTCAGCAAAGTATGTTCCTAATTCAACCATGTCTTGAGCAACAACTGAACCTGGTCTATCAAAGTGAAGAGCAAATTTTCTTCAGTCTCCAAGTTTCATAACTTTAGGATTAATACCACCGTTTGTAATAGGGTTCATGCCCATTCTTCTTTTGCCTTTAGGAGCAAGAGCAGCGATTTCTTTTACCAATTTACCTTTTTTATCAAATAATTCTTCAGGACGATATGATCTTAATCATTTTTCAAGTGCATCTGCATGTTCCATGTTTTCTGCATTAATTGGAAGTGGAACTTGGTGTGCACGGAAGCTTCCTTCAATAGCTTCTTTGTTTCATGATTTAGGACCTGTTCAACCTTTAGGTGTTCTAAGAACGATCATTGGTCATACTGGTCTTTTAGCTTTGTCAGCTGGTTTTTTACGAGCTTTTGTTTGAATGCTTTGAATTTCTTCAATAACTGAGTCTAAAGTTTTTGCCATTAATTCATGCATAACTTCAGGTTTGTCGCCTTCAACAAAGTGAGGTTCTCAGCCCATACCTCTAAAGTATTGTTGTAGTTCTTCATTACTTTTACGGCTTAAAATTGTAGGGTTTGAAATTTTTCCACCGTTTAAGTGAAGAATAGGTAATACAGCACCATCATTTACTGGGTTAATAAATGAGTTTGAGAATCAGCTAGTTGCAAGAGGTCCTGTTTCAGCTTCACCATCACCAACGATAGTAGCAGCAATAACATCAGGGTTATCTAAAATTGCACCTGTTGCGTGTGAAATTGAGTAACCTAATTCTCCACCTTCATGAATTGATCCAGGGGTTTCAGGAGCAGCATGTGAAGCTGTTCCACCAGGGAATGAGAAGTATTTAAATAAATGTTGCATTCCGGCTTCATCTTGTGTTACATGTGGGAATAATTCTGTGTAACTTCCATCTAAGTATGAGTTAGATGTCATAACTTGGCCACCATGTCCTGGTCCTTCAATGTAGAACATGTTTAAGTCATATTTGTTAATAACTCTGTTTAAGTGTGCATAAGCAAAGTTTTGTCCAGAAATAGTTCCTCAGTGACCAATTGGGTAAACTTTAACATCATCAGAAGTTAAAGGATGTTTTAAAAGAGGATTATTTCTTAAGTAAATTTGACCAACTGAAAGATAGTTTGCAGCTCTTCATCAAGCATCAACTTTTTCAAGGTATTCTTTACTATCGAATTCTTTTTTGTTCATTTGATCTCCTTATTTATTTTTTGCATTTGTTAGTCTTTCTAAAGTTTAGGCTAAATTAATAACCAGAAGAATAGAATAGACAAGTTTATACTCTCCCGCTTTTGTTACTTACAAATTGCAAAAAAATTATATATTAAATTTAAAATTTATATTGATAATAAATTATTATTATTTGATTTTTTTAAAAATAACAAAAAATCCTAGCAATGCTAGGATAGAAATAAGTAAAAATTGTATTAGAAACTGCTTTCGTATTCTTTGGTGTCAATATCAAAGTCATCTGAGAAATCGTATTCTTGAGTTTTGCTTTGTTTTTTGTTTGTTTCAACATCATCTTCTTGAAGCAAACCGAAGTAGAATACACTTGATGTATCAACTGAGCTTGATCTTGATGAAAAGTTTGTGGCTATTTGATAAAAGTGTCTAAAATCTTTTGAGTTAAAGAATTCTAAATCCTTTTCAGTAATTTGAGCTAAAGGCTCAAGCATGTACAGTTTGTCACTAACTGCAACATTAGCAGGTTTAGGAACAACTGAAATTTGGTTTGTGTTCATATAAAGAATGTAAAGGTCTTTTCTTTCTAAAGTTTTAGCTAAATTAGTATTTGGTTTAATTAAATTTAGACTAACAAATAAGTCAGTGCCATTATTGTTTTTAACATATTTGTTTTTGTTATTTAAATTTAATGGTTGAATTAATCAAACCGAGTTACTTTCATTGTAGGTTGTTTGAGGAATTGAATTTTTCATGATATTTAATGAATTGAAATTCATTCTACGAGCTATTGAGTTAAAGAAAGTATTACGGTAAAGTATTCAGTTAGGATATGTTTTTGAAGTTAAGAATCTTTGATTAATTTCATGCAATTTTTCAAATTTAGCTGAATATACAACAGTTGTATTGCTCATTGAAACTTTTTTAGCTAAATTAGCAAAAATAATAATGTTTTCATTACTAATTCCGTTGTAACCAAGAGTATCTAAATCTACAATGTGATCAATTGTAAATTTTGAAATGTCATTTCTTAATTCTTCATATTCATTTGTTGATAAAAAGTTTTTAGTTAAAACTGTAACAATATATTTGGCTCATTTTTTACTTTGCATTAAGTATAATGGAGCAAAACTTTGTAATAATTTGTTTTCTTTAGTTAATGAAGGATATTTTTTTCTTAAAGCTGGATGAGACATTCTTATGTTAGGGAAATCACCAATTAATAAGTCAATTGGTTCATTAGTTCTTTTCATTAGAAAATCAGCATTTTGAAAACTAACACCAAAAGTAACTTTAAGTTTTATCATTTTAGATAAAGCGCGAGCAATATTTATTGTTTCATTATCTACATCATAAAAAGTAATACTTAGTTTGTTTTTGTCTCCATATTTTTCAATAATTAAAGGTAAGAAAATACCAACACCACATAAAGGATCAACAATCGTTAATTCTTTGCCTCTAATATTTGGAAGTCTTTTGTAAATTGAGTTTAGTAAACTTTCATCTGTATAAAAAGGTTTTTGACCTAATCTCAAGCAGTTTGCATATTGAGCTACTTGGTACATTGATCTAACATCCATAAGTTTTGGATTTTTTTCAATATATTTAATTAAATATTTAACATCTGTTAATTTTTCTTTTTTGATGATATCTTTTATGTTTCTTTCAGTTAGTGAACCAAACATATTTTCTCCTATTTTGTCTTTTTTACTATAAAATAAGCATTTATAATCAATTTTGTGAGTTAAATTATAATTTAATTATAATATATATTTTAGATTAATATTATTAATATTAAAAAAAGTGGTGTAAAAAATTTTATTAGGTAATTTTTGACAAATTTAGCTATAAAACAGGACTTTTGGTAAAACGCAAAAACAATTTTAAAAAATATTGCATACAAAATTAATTTTTTCTATTAAAATTAAATATGCAAAAAATGTTTTTATATTATAATTACATTGCTTATGCAAATGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATTTTGCCCCATAGCCAAGCGGTAAGGCAACGGGTTTTGGTTCCGTCATGCGTTAGTTCGAATCTAACTGGGGCAACCATATCGCTGCGTAGGCGATTTTTTTATTTTTATTTTTTTACTTTATAATATATATGTTATGAATAATGATTATGAACAAATAAAAGACGATAGCTTTAAAACAACTAACGATGTCTTTGTCAGAAGAAATAGTTTTTTAGGTTACACATTACTTTGATTTGCATTTTGCATGGCAATTAGTTTCGGTACAGCATTTGTATGTACATTGTATATGGAAAAAATTGCTGGCTATTTTGCAAATAATGTTATTGGATGAATAATTGGAGGTATTCTAACAATAATTTTACTTTTTGTATATATGTTTTTAGGACCTAGAATGCATATAGGTGTATCCGTACCTATGGTTATGATCATAATGATGGGATTTGGTCTATTTGCGCTTTCTATTCCTCTTTACTATGCAAAAGAAACCATGGTTTGATGAAAATTATATTTAGTATTATTCTTGCCAGCAGGTTTTATGTTTATTATGGGAACTTTAGCAGCAACCAATAAAGTTGATTTAAGTAAAATTTGAATTCCTATTATAGTGGTAGTAGTAACAATGTTGATTTTAGGTATTGTATCATGATTTGTATTTTCAAGTTATATAGTTGCTTTAATTTCAGCATTAGGTGTTTTATTAATGGCACTTTACATGGGATTTGATTGATACTTTATTAGCAAATACAATAAAGTTTATAACAACTTTATGGATGATGAAGAAACTAAAAAAGAAATGATAAAATTATCTCTTTTCTTTGGTTTCAAATTAGCATATGATTATATTTATTGTGTACTTTATTTAATTAGATTATTCTCAAATATGAAAAATTAAAACTAGCCCCTTTTTAGTAAGCTAAGCTTATTTATTGAGCTAGTTTTTTTAATTATTTTTTAAGCTAAAGAAACTTCTGCATTGTAAACAATTGGATCATCCAAAACAGGTTTATTGTTTTCAAATTTAGCTAATTTAAAGCTAAATTTTACAGTATTGCCTTCTAGTTTAAAATCAAGAATTGAAGAAATAGCTTCTCCTTTTTTGCCATTATATGTAGGTTTTTTTGCATTTGCAAGTTGTACACTATTTTTTTCAATATGTTCTTTTAGTATGCCTGTTATATTTAATGCAACAACCTTGTCTCCACTTTTTCAATTAATTTGTCCTTTAAAGCAAACAATGTTGTGGCCTTTACGATCATATGCAAATGAATATGATTTAGAATCTTTTTTTATGGCTTCAATAGCTTCTTTAATTCTATCTTTGCCTTCTCCTAAAGTAAAGCATGTTTTATATTTGTCTTGTTCAGATGTACTTCCGCTTTCAACTTTAGCAGCAACTTTTTTAATTTTTGCATCTACAGCTTCAAAATCTTTAATTGTTTTAGCGGCATCAAAATCTTTTTTAATAGCTTCTACTTCCTTTTTAAGTTCAGCTTTTTTAGTTCCATCAGCAACAGTTTCAATTTTAGCATTTAAATCTTTTAATGCTTGTTCAACTAATGCCTTCTTATTAGCAAGTGAATCAGTAGGTGTTTCTCCACCTTTAGAACCACATCCAGCACTAATTGCTACTGGTGCAAAAGATAAAATTCCTGCAATTGAGCCTAATTTTAAAAATTTTTTATTCATAGATTTTCTCCTCATTATTTTTACTAAATAATTATACTATTTTTTATATATAAAAATTAAAAGTACCACATAAATAAAAAAATGGCGTCCTTGAGTGGAATTGAACCACCCACCCCAAGCTTAGGAGGCTTGTGCTCTGATCCATCTGAGCTACAAGGACATATTTGTATTATATAACAAAAAATAAAAATAAAAAAATGGCGATCACGAGAGGATTCGAACCTCTGACCACGGGCTTAGAAGGCTCGTGCTCTATCCTACTGAGCTACGCGACCACGCTATTTACAAAATATTTATTTTTGCATTTATATATTACCACAATATTATTTTTTTTATTATTTTTTTGTTCTAAAAAAATGTTAGCCTCTAGTTTTAAAGAAACAGCAAATTTTACTAAAACAACTATCAAACAATGATATTGGTTGAAAATGTATATATAAATTTGCAATTTTTTGTGTAAAAAAATATTGGAATTTTTTAAAAAAATTATATTTTTACATAATTTTATCGTGATAATAAATAGTATAGAAAGGGTATTTTAATTAAGAAACTAATTTTACTCAGTGTTCCACTAAGAATCGCTGCTTTAGTTCCTATAGCTATAAGTGTCAACTATGATAGTAAAGAAAAAATAGATGTTAAAAACACAAAAGCTCAAAAACATATATTATAAAAATTGTATGAATATGAAAAAAGAATGAAAAAAATTAGAACCTGAAAAAACTGACGTTTCATTCTGATTACGTTTAGGTGTTGAAAATAGTAAGCACTAAAGCAATTTTAGATAAAGAAAACCAGATCTTCAAGAAATGGAACAAGCATTTGAAAAATTGCTTTTAGTAGCCATTAAAGCTCTTGAAAGCTTAAGGTCTTAATTTATAAATTAAAAAAATGCCTCTGAACTGATTTCAGAGAGGACTGTAAAAAATTTTGTGTAAAGTTCTAAAATCCTTTCTTAGTAGATAAAATATTATTAAGAAAGGATTTTTTATTTATGAAAAGAAAATCAATAAATCCTATTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTTAAATGGAAATTGACATTCCAAGAGATCATGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAAAGATGTAAGTGATTTTTGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATAGTGAAGGACGAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATACCCTTAAAAATATAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCCATTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATTTGCATATCAGTCTAATGATACAAAAAATGCAATGGATAATTTGGATATTTTTGAAAATAAATGAGGCAAAAAATATCCAACATCTATTTCAATATGAAGACGGAACTGATCTGAAATTTCTACAATGTTTGATTATTCTCCTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTGATTTTTAAAATAAAAAATAATTTTTTTAAAATTTTAAAAGCTTTGCTTCTTTGACTTCAAAGAAGGCCTCTCCCTTAAATGAATTAAGAAAGAAGGGAGAGGAATTAAGATTTCTTGCACAACAAGATCTTTTTCCTATTTTAAAATTCGAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTGCAATATTATTAGGTGTTGATGTAAAGTCAATTCCTTTCTATTGAAAAAAGGAGAGACTTTACACAAAATTCTAAACAGTATCACTGCCTTAGAAGAATATATTTTATATTATAATAATGATATAATTATTACTAAATTAAAAGGACTTAGCCCTGTTCAATATAGGAAAAAGTCCAATATTTGGGGTTCATACCAGTTTTAGTGTAGTCTTTTGACTGATTTCAGTCTAATCGTTTTTATCGTAATACTCTAAAATATCAAAAAATTTTCCAAAATCAAAATCTTTTGCTTTGTCAGGATCATCTGTTTTAGAATAATAATCATTTACATGTTTTAGTGTACTATACGCGATTAATGTTTGTCTATTATTTGATACATGAACAACACCAATAGTTAAGGAATCGCTGTTTTTATCCAATTTTTCGATAATATCATCACTTGTGTATTGTTTATAATTTCTTAACATTTTAAGGGTTTTATCTTTATCTATGTCTTTTAGGCCTTTTATTTCGAGAAGTATTTGGTGATTTTTATATTTTATTAATAAATCAGGGTAAGAAGTTTTAACAGTATTCATATTTTCATCAAAATAATTAAAAGATATTCCTTTAAAAGTTGGATTTTTTGTTCAAATATTGATTTTATCTTTTAAATTAAGCTGACTATTAAAAAATAAATTTTTGAAATTCCCTAAAACTTGAACTTCATTTTTACTATCCAAATGGTGAATACCTTCATTTTTTTTGTTTGTATTTAAATACGCATATTTTAAAAAATATTCATCAAATGATGGCGATTGATTAAATTTTAAGCAGTTATTTGAATTAGAAACATATTGATAATCGTCAAATGGCAATTCTTTTGTAATTAATTTAAATTCACTAGCTTTTTTTAATTTTTTATAATCTTGGAAAAATTCTTTAATTTTGTAAGAATATTTTTTTAAAAATATAAATCATAAAATATCATTATCTATTTTAGTTTTTAAGTCATCAATTATGCTCTTGTTAAATTCGTCAAATTTTGATTGAATTAATTCATTAACTTTTTTGTTTAGAAATCTTTTTAAACTATTTTTAAGTTTCATTATTTCTATTCTTAGTTCAATTTTGTTTTGTATTATACTTTCAATTATTTTGAAGTTGTAATTATCATTATAAAGTTCTTGTATTTTATTTTCGATGAATCCTTTTTCGATATAGATGTTTTTATAATTTTTATTGTCTAATAATAAATCCATGTTGTCAAGGTCATTATTTAAAAAATTTTGAACTTTTGAAATATATTCTTTGCTATTTGAAATTTCTTTAATTAAATCTTCGTCTATAATACCTGAATAAAATTGAATAGTTTCATTTCCTTTTTTTGTTAAATCATCTCTAAGAATGTAGTAATCTCATAAACGTTTTTCATCTTTGAAGTTTGAATATAAATAATAATTAAATTTAATAGAATTTATATTTTCAAAAGATTTTTCAACTGGTGCAGGATTTCTTTTTATTCTACCAACTGTTTGAACATTTAAACTATCTGATGAAACATTTCTTAATTGAACTAACATACAAGCTCTTGGTATATTTCAACCTGTTGCAGGGCCTATTTTAAAAATAATTGCATCTACTTCAGAACTATTTTTAGAAATATATTTTAAATTTATTTTTGTTCTAATATTTGTTGCATTTTCAGCAACTTTTTCATTACTAAAATATTTAACTCACGTAAGACCATTATTCTCTAGTATTTTTATTATTGAATCAATATTGTTTTTAAATTCTTTTTTCAAATGTGGATCTTTAGGTTCGTCTTTTACTTGTATTAACATAGCAGGGTTAATTCCGATTAAAGAAGGTTCTTTTTCAGAATCCATATATTCTTTTTTAATTGCTTTAAATTTTTTACATGCAATATCTAAAATTTCATCATTTGTGTATTCTTCTTTTTCATAGTCTTTCAAATCATTAAAAACAGGACATTGTTTCAATAAATATATGTTATCTTTTTTCAAATCGTCTTCATCGATTCTTATTTGTTTTATGTTGTATTCTTTAGGGGTTGCAGTCATTTTAATAATATAATGTGCAATTTTTTGCATTTTTGTTTCAAATCCACTATCATCTTTGTTTTTTTCAACTTTTCCACCATGATGAGCTTCATCTCTGATATAAATCAATTTATAATTTTCGTTTTTTATTTGTTGTAAAAAACTTTCCAATTTTCCTCTTTCAACCAGAATACAATTTTTTTTGAAACTTGCATTTCCCAAGATTATAACTTTATTTTTTTCAGCAATCATAGAAACATCTTGATCTTTACTTTTTAAATTTTTAACACTTGGGGAAATTATTCTTTCTATTTTTAAATTTGAAGAATAATCTAAAAACATTTTATATTCATTTAAATTATTTTCCATTTGTTTAGGCAATTCACTTGAAGAGAGTGTAGCAATAATAAATATAAGTTTTTCATTATAATAATTTTTAGCTAAATCATTAATAAAATTAGAAATCATAAAAGTCTTTCCACTTCCTGTTGGAGCGCTAAAATATACTTGTATTTTTTTGTTTGGATTAAAAGATTCTAACAATTCATTAACAGCTTTTTGTTGACTTTGTGTTAATTGCATTATTTACCTTCACTTTTTTCTATTGGTTTTAAAGCAAGTAAATCATAGTAGAATGTACTTATATCAGTATTTTTGTTAATTTTTACATTAAAATCTTTTAATTCTTTTTCATAAATTTCCACAATTTTATCAAGTGAATCAGTTTCATCAAATAATTCTGTTTTATAGTATTTTATGTTAAAGATATCTAAATTATTTTTATATGGTGTGTTCTTTTTAAGTCATGCAAAAGAAGTACTATTATCAGTGCCTTTGCCATTATTGATTCTATATAATCTCTCATAGCAAATTTTTGTTCCTACATTGTTTTCGTCATTTGTAACAAGCGTAAACTGTCTTTTACCGCCATCCTTTTTGTTCATTTCTAAAATTGCATGTCCTGTAGTTCCTGAGCCAGCAAAAAAATCTAAAATTCTGGCTTCTTTATTATTCAATTTAATATTTATTAAATAATTTATTAAATCTATATTTTTACAAGTGTTAAATTTGGTATTTTCATCTAATTCTATATTTTCACAAATATTTTCTAAAAGATCATTACCATTTTTTGTATTTAATTTTGGGCCCATTATTATTGATTTTGGTGTTCAAGAATTGTTTCTTTTTTTTGTTCAAATAGTAAATTCATTTGCTTCAAATTTTACATATAAATCATGATTGTCTTTAACAACTTTTTCCTTTTCTCATCTTCAAACACCGTCATAAATATTTTTAATTTTTTTAGGAAAAATTTCTGCAATAGTTTTTTCGGTTTTTACAACATCAACTCTATATTCGTTCCTATCCTTATATATGTATAAAGGATAATACAAGTTGCTTCTTGTTTGTTTGTTAAACTTGCTGTTTGTATTTTCTAACCTATTTTGTAATATAAAGTAGCCATATGTATCTTTAAAGATTTTTTTATTTCTTTCTTTATATATTTGAGGCATTAATTCTGTTGCATATTTGTCTTCTAATTCTTGGAATTCAAAAGAATTACTTTTTGAATAACATAAGATATATTCATGTGTTAATGCAAAATTTGTGTTTGCTTGTCTTCCTTTTAAGTTTGAAATGTATGGCATACAAGCTATAAAATTCTCTTCTCCAAATATTTGATCCATTAAGATTTTTAAATAAGCTTGTTCAATGTCATCAATTGACACAAAAATAACACCATCTTCTTTTAAAAGATCGCGAGCCATTATTAGTCTTTCATTAAGCATATTTAATCATCCATTTCTAGAATATTTGTCTCTGTAAATGAATTTGTTAGCTTCAATATCTTCTTTGTCATTTGCTACAGCATTGCCATCATTTTTTGAAGCTTCTGTATTATAAGGAGGATCAATGTAAATTAAATCATAGAATGGGTTAGTCCCATTCTTCTCTCTCTCTCTCGACTATTAAAAGATTCTTTAAAGCGTCATAATTTTCACCTATGATTAAAATGTTTTGATTATCGATATTCTTTTTGTCATTAATAAATGATTTTTCTTCATCTTTAACAAGTAAAGCAACAGCTTTTTGATCTACAGATGGTGCTGCATCAAAAGTAAATCCAACTTTAACTCTTTGAATTAACATTTGATATATATTTTGTAAATCTGCATCATCTGCTTTTTCTAATATTTTTTTAAATAAATCTTTTTGATCTTGATTAACTTCAGCTTTCGCAAGTTCATCTATTTTTTTATTGTATGAAGCTCTTAATTCTTCATTTTTACTTTTATGTTCTTTCATAATTGCCTCTCCTTATAATAATTTGTTTAATTCAATTTTACATTAATTAAATAGCGCCGTATATTAAAAATTATTTAAATACTTCAATTATTTACTTTATAATTAATTTTAATATTAATAACAAGGAGAAAAACATGGCAAAATACACAGAACAAGAACAAATTCGTCGTGATAAACTAGAATTTTATAAAAAATTTGATGTAGTACCTTTTAAAAAAGCTTATGGTCTAGGTAAATTGAGTACAAGTGAAGAACTCAATAAAAAATATGGTCAGTTTACTAGAGAAGAATTAGAAGTAAAAAAAGTTAAGAAAAATATTAGTGGCAGATTAATTACAGCTAGGGGGCCTTTCTTAGTATTAAAAGATCGTAAAGGAACAATTCAAGTTTATTTCAATAAAAAAGAAAATCCTGAATTAACTAAAATAGTTGAAACATTTGACTTAGGAGACATTTTATGAGTTAGAGGTTTATTAATGAAAACTCATACAGGTGAAATGACAGTTAAGGCTCAAGATATTCAATTGTTAACTAAAGCTCTTAAACCTCTTCCAGATAAATTTCATGGATTAACTGATACTGAAGAAAGATATCGTCACAGATACTTAGATTTAATTACTAATCCAGAATCAAGAAATACATTCATTATGAGAACAAAAATTGTTCAATGAATTAGAGATTACTTTAATAAACTTGATTATTTAGAAGCTGAAACTCCATTTTTACATGATTATCTTTCAGGAGCAGCTGCTAAACCATTTACCACACATCATAATTCATTGAACCAAGATTTTGTTTTAAGAATAGCAACAGAAATTCCACTTAAAAAATTAGTTGTTGGTGGATTTGAAAGAGTTTACGAAATGGGTCGTATATTCAGAAATGAAGGATATGACACAACACATAACCCTGAATTTACAACTATTGAATTCTATGAAGCTTATTCAAATGTTGAAGGTATGATGAATAGAACTGAAACATTAATTAAAGAACTTTGCAAAAAAATTGGTAAAAGTAAATTTGTAACAAATGGTGTTGAAGTTGATTTATCAAAACCATTTAAACGTGTAAATATGATTGATGAAGTAAGCAAACAAACAGGTAAAAACTTTAGAAAAATTACTTTAGAAGAAGCTATTGAAGTAGCTAAAGCTTACAAAATTAAAATCGAAAAATTCTTTACCATTGGACACATTATTAATGCTTTATATGAAGAATTGGTTGAACCAACATTAATTCAACCTACATTCTTATATGGGCATCCAATTGAAATTTCTCCTTTAAGTGCTAAGAGCGATGATCCTAGATTTACTGAACGTGCTGAATTATTTATTAACACAAAAGAATATGCCAACATGTATACAGAATTAAGTGACCCAATTGATCAGTTAGAAAGATTTGAAAGTCAATTAGAAGAAAAGAATAAAGGTAATGATGAAGCAAGTGATATTGACTATGACTTTGTTGATGCTTTAGAATATGGCATGCCTCCAACAGGTGGTTGTGGAATTGGAATTGACCGTTTAGTTATGTTATTAACTGAAACAGATTCTATTAGAGATGTACTTTTATTTCCCACACTAAAAAGAATCAAAAAATAATAATATTATTTAATGCCCTAGATAAAAGGGTATTTTTTTATCTTTTTTCATAAAAAGCAAAAGTTAAAAATACATCTCTTTTACCAAAACAACTGTTTTATAGGCTTATTAATAAAATAATCAATGAATTATTGTGTGAAAAATTTATAAAAAATATGTATTTTCAAGTGATTTTTATCATTTAAAAAATACAAAAATAACATTATTAAGCATTTTTAAACATAATTTAAATATGCAATAAAAGATTAAAATTGACCAATGAATTGAATCATGTCAGGATAAATCTAATAAAAGGTTTTAAAGTTTTATAAGGATTAACAACTACTACAGCAACAGCTAAAGGCGCATTGTTAATTGCTTTAGTTGCTAGACATAATCAAAAGAAAGAGAAAAACAATAAAACCAGCTAAAGATACATATTTAGAAAATAAACAACAAATCGAAAAATGAACTAATAAATTAGTTAAATATAGCATTGTCAAAGATTCATATCCAAAATATGAGACTGAAATAGTTTCAATATAAAATCTTGCTAATGAATCTAATAAAAAAAGTTGCTCAAGATAAAAAATATTTTAGCAAATGGCCCAAATAATCTTTTAAACAATATTAAAAGTTTTGAGTTAGCAAATGTTAGAGCAAAAGAAACTTATGACCAAATTAGACAAATGAGAAAGTCACATTTAGACAAATATTACCTAACATTGTTACAATTGTTGAATATAGTAAAAAACATGATGTGGCAACTGAATTAAGACATGTTGTAGAGTTAATGAAAGTTGTAGACAATGATCTTTCAACTTATTTCAATAGTTTAACAAGAAATTAATTAAAAGGTAATTTTTGTCCTAACGGTTTATATAAATTAGTAATGGATGATATTGATAAATATAATAATGCAAAAAAGTAGAAACTTTAGGCTCAATAAGCTAAGATTTTTATTTTGCTTGTCAATTTTTTTATACATTTATTTTCTTATAAATGCTTTAAGTTTAAGTGTGTTTTGAATGAAAAAATATTTTTTGGTTTTTAAATAAATTAGTTTTAAAAAATGAGAATTTTTCACATTTTACAATCTTTTTAATTTTGTTAATTTTTAGAGATTTAAAAATTTTAAAAATTTGAAAATAATTTTCAATTTATTTATGTATTTTTGCTTATATATATTATAAGGTATCAAGAAAAGTAAAAAAAGCTGTAAAGATTTTCATATAAAAATTTTGGAAAAAACTTTCAAAATACGCAAATTTTATTTATTTTAATTATTAAATTTATTATCTAAAATAAGTTTAGAACCAAAAATCAAAAAGGGTTCTATTGATTACAAAAAAGGAGATTTATGAAGAAAGAAAAATTTGTTAGGACTGTGCTAGGCGATGTGCCTGCTAGTTCTATCGGTATTACTGACTGTCACGACCACCTTATAAAGAACGGTGGCCCTGAAATGGAAGAACACATTGACTTTTTAATGATCGATGTTGAAGCTGCTAAAAAAGAACTTCAAGAATTCGTTGATCACGGTGGAAAAACAATGGTTACAATGGATCCACCTAATGTTGGACGTGATGTATTTAGAATGATGGAAATTGCTAATGCATTCAAAGGCAAAGCAAACATTATTATGTCTACAGGTTTCCACAAAGCTAAATTCTATGACAAATACTGTTCATGATTAGCTAATGTTCCAACAGACGACATTGTTAAAATGTGTGTCGCAGAAATTGAAGAAGGTATGGACGTTTACAACTATAATGGACCAGTTGTAAAACGTGGTAAAGCTAAAGCTGGTATTATTAAAGCTGGTACAGGTTATGCTGCTATTGACCGTCTTGAATTAAAAGCACTTGAAGTTGCTGCTAGAACCTCAATTACAACAGGTTGCCCAATTTTAGTACATACTCAATTAGGTACAATGGCTTTAGAAGTTGCACAACACTTAATTGACTTTGGTGCAAACCCTCGTAAAATTCAAATTTCTCACTTAAATAAAAACCCAGACCCATACTACTATGAAAAAGTTATTAAAGATACAGGCGTTACACTTTGTCTTGATGGACCAGACAGAGTTAAATACTACCCTGACTCAACTCTTGCAGATAACATTAAATACTTAGTAGACCAAGGTCTTCAAAAACATATAACATTAAGCCTTGATGCTGGTAGAATTCTATACCAACGTAACTATGGTTTAACAAAAGGTAAAGAAACATTTGGATTCTCATACTTATTTGATAGATTTATTCCTTTACTTAAAAAAGTTGGTGTTCCTCAAGATGCTATTGATGACATTTTGATCAACAACCCACGTGATATTCTTGCATTTGATGAACCTCGTAAATATGAACCATCAAAAGTATCAAACGAATTAAAACAACTTAAAAAAGATCTAAAACTAGATTAATTTAAACTTTTATATATTTATTAAAATTAAACTTAACTTAATTAATTATTCATTTATTTTTATCCATAAAAACTTAAAAACATAAAATTACTTATAACTTATATACTTATTTAACTCAAAATAATTCCACTTTTCGGGGGAAGGATATCTTATGTCAGAAAACAAGACAAAAAGATCTTTTAACTGAAAAGCTTTAGTTGGTGCTCTTGTAGTTATCGCTATTTTTGCAGGATTCTATGTTCTTGCTTTAGGTCTCAAAAAATGAGACTTTAAAGCTGGTACATTATTCTTTGCAAAAGACTTATTATTAAATAACTTCTTAGGAATTAACGCTGTATTAATCGGATTAATTGTCTTCATTGGCTATTTAATACTAGGTAGAGGCTTTTCAGACTCACTTATTGGAATGCTTAAAGCCATGATTGGTGTCTTAATGATGCAAATCGGTTCAGGAATGATTGTTGGTTTAGCTAAACCAGTGTTCCTTGCATTCTCTAAATTCGGTAAAGGGCTTACACCACTTGATACATACTTAGGACAAGTTTCAGTAGAAAACTTCCTTAACTCAGTTGGTCAAGGTTTTGCTTCATGATTAGCATATGCCCTATTAATTGGTTTAACAATTAACATTATTTTAATTGCATTAAGAAGATGAACAAATGTTCACTCACTTATGATTACAGGACACGTTATGTTCCAACAAGCAGCTGTTGTTGTTCCAGTTGTACTTGTATTAATGTTCTCAGGACAACAATTTAGAACAGCAGAAGGTGCTATTAATGTAGGCGGCCAAGTCGGTACAATCTTCTTCTCAGGTTTATTACTTGGTGCTTACTGAGGTGTAGCTTCATCTTCAACAATTAAAGGTTCAGATGCTGTTACACAAAAAGCTGGTTTCTGTGTTGGCCACCAACAAATGTTCGGTATTGCTATTGCATACCAAATTGGTAAATTCTTCGGTAAAAAAGAAGATTCAGCAGAAACTAAAAAACTTTCAAACAAAGTTAAATTATTAGAAGACAATATCTTCACACAAAGTGTATTGATCTTATTAATTTTCTTAATTCTTATCTTAATTATTCAATTCGCACCTGCAGGTGCATCAGTAAGATTTGGTTTAAGCAATGGACTTGTAAACAAAGCAATTTACGGTTCATGAACACCAGGCGGTGGAGCAGTTTATTGACCAATTAACCTTGTTTTAGGTTCATTACAATTAATTGCTGCTATCCTTACATTGCAAGCTGGTATTCGTATGTTCGTTTCAGAACTTCAACAAGCTTTTCAAGGAATTAGTGAAAAATTAGTTCCAGGATCAGTTGTTGCTGTTGACGTTGCTGCCACATATGGTTTCAGCCAAAACTCAGTTACCTATGGTTTCTTATTTGGTTCAATTGGCCAATTCTTAGCTATGGGTCTTGTAATTGGATTAGCTCAAATTCCAAACTCAAGATTTGAAATTGTTATGGTAGTTCCATTATTCATTACATTGTTCTTTAACTCAGGTTCAATTGGAGTATTTGCAAATGCTTCAGGTGGATATAAAGCTACTATTGCAGTACCAATGATCTTTGGTTTTGTTGAAATCATTGTTGTTGCTCTTGCTTTAAGTATGATTAAAAACTTTGGTGTAATGCACCAAGTAGATCTTGACTTCCAATACTTTGAAAAAACTCTCACAAAAGCAGATGCATTCAAATCATTTACAGATTCATTAAATGCAAATTCTGCTAACTTGTTTATTCAAGGTGATGCTACTAAAGCTGTTTCAGCAGCACAATTAAATGATGCTATTACAAACATTAACAGTCAAGATTCATTCAAAATCTTAAGAAGCTTAAGTGAAGTTACAACAAACTGAACTTTAGGACATGGTAAAAATGCTAAAGCTATATCTCTTCCAGATATGCAATACTTATCAAGATATGTTTCAGACCAAATAGCTAAAGCTAAAGATATGAAAGAAATCTTGAAATTATCTTCATTCCAAAGAACACCATTCTTAACAGGATTTAATGGTATGTTTGACTGAACATGTATCTTTGGTTCAATTATGATTCTTGGTGGATGACACCCATATGCAGCTTACATCGTATTCCCTCTTTACGTTGTTGGTATGATCTTTGCTGCTCAATTCTTTGACTCACACAGACAACACAAACCTACAGTATTCCAAAGAATGCTTAAAGTTAAAGTTGAAACTTTAGAAGATCAAAAGAAAGCTGAAGAAGTTGCTAATGCACCAGTTGAAGAAGTTCCTCAACCAGAAGTTGCAGAACAAGCTGCTTAATTAGGCAAAAAAGTAAAAATAAAGGCAGAATTTTCTGCTTTTATTGCTTATATTAATATAAATTATATATATTAATTATTAAAATTTAGGAGATTAACATGAAAGTATTATGTGTTTGTGGCTCAGGTATGGGCACAAGTATGATTATTAAAATGAAAGTTCAACAAGCTATGAAAGAACTTTCAATTCCAGGAACAGTTGAAGCTTTAGGTTTAGGTCAAGGTAAAACAGTTGTAAACAACTTTGATATTGTTTTATGTACCTCAAACTTTGTTTCAGAAGTATCAAAAGGCAAAGCAAAAGCTTATGGTTTAAAAAACATCATGGATGTTAATGAAATCAAAGCTGCTCTAAAAGATGCATCAAGCAAGATGTAATAATGGTTGATAAATTAAATTTATTAGATAACTTAATTGTTAATGATTCAATCGTACCTCATGTTGATGTTAAAGATTGAAAAGATGCAATTAAGAAAGCTTGCTTACCATTAGAAAAAGCAAAAGTTATTAATTCTAAGTATTATGACTGCATTTTAGAGTCAATTAAAAAACATGGACCATATTTCATTTTAGTAGATGGTCTTGCTATGCCACATGCTTCAGCAACAAAAGACGCTGTTTTTGGAAACGGATTCTCACTTATTACTCTTAAAAAACCTATCAAATTTGAAGGAGATGAAAGAGAAGTAAAAATCCTTATGGGACTAGCCGCAAAAGATGGTGAAACTCATACATCTGTTGCTATTCCACAAATTATTGCTGTTTTTGAAGATCCACAAAATATTGAAAAAATAGCACAAGCTAAAACAAAAGAAGAAATTATAAATATCATAAAAGGTGTTGATTATACTAAATACCTTAGTTAGGACTTAATATGAAACCAATGTTACAAATTGCTTTAGATAACTTAACAATTGAAGATGCAATTGCAAGTGCCAAAAAGGTTGAAAAATACATAGACATTATTGAAGTAGGAACTATTCTTATTGCTTCAGAAGGTAAAAAAGCTATTAAAGCTTTGAGAGAAGCATTTCCTACAAAAATCATTGTTGCTGATGGCAAAATTGCAGATGCTGGGAAAGTTTTTGGAAAAATGTTTTTCGAAAATGGTGCTGACTTTACAACATGCATTTGTGCAGCTGAAGTGCCTACAATTGTTGAAACAATGAATATAGCCAAAGAATATGGCAAAAATAAAGAAGTTCAAATTGAATTAACTTCAAACTTTACTTGAGATCAAGTTGATGATTGAGCAAAAGTAAAAGTTCCTCAAGTTGTTTGACACCGTTCAAGAGACAGTCAAGCTAGTGGAGTTAAATGAGGACAAAAAGACATAGATGCTGTTGCAAAACTTGCTAAAAAAGGTTTTAAAGTAACTATTACAGGTGGTGTTGCTCTTGAAGATATTAAACTATTTAAAGATATTCCTATTTACATCTTTATAGCTGGAAGATCATTAAGAGATGCTGAAAATCCTGAATTAGCTGCTAAAGCCTTTAAGGACGAATTTGCCAAATATTGAAAATAAAAAAAGACTTCTAGGCATCTATGAAAAAGCTATAAACAACAAATTTAGCTTAGAAGAAAAAATTATAATTGCTAAAGAAGCTGGTTATGACTTTATGGAATTTTCAGTTGATGAAAGTCCTCAAAGATTAGCTCGCTTAGACTGAAGTGATGAAGAAATTGCTCATGCTCAAAGCTTACTAATCAAACACAAATTTAACTTTAACTCAATGACCCTTAGTGGTCATCGTAAATATCCTTTTGGTTCTAAAGATCCTAAAATCAGAGCTAGAGCCAAAGAAATAATGACTAAAGCAATTATTTTAGCCAAAAAATTAGGTATTAGAACTGTTCAACTTGCTGGTTATGATGTTTATTATGAACCAGGTGATGAAGAAACAAGAAAAAACTTTATTGAAGGAATGAAATGAGCAGTTACTGAAGCAAGTAAATATTCAGTTATGTTAGCTTTTGAAATTATGGATACTCAATTCATGGGAACTAATTCAAGAGCAATGACTTATATCAACATGATTAATAGTCCATATTTACAAATCTATCCTGATTTAGGTAATATTTATCAATGAAGTAAAAAAGAAGATTTGTATAACGAATTTGAACTTGCTAGAAATCATATAGTAGCTTTCCACTTTAAAGATACTGTACCTGGTAAATTTAGAGATACACCTTTTGGAACTGGCACTGTAGATTTCCCTTATCTTTTAAAAATATTAAAAGATTTAAAAATGAACCAACCTATTATGATAGAAATGTGATCTTTAAATAAAGAAAATGAAACAAAAACACAAGCTATAAAATACATTAAAGATGCTCATGATTTTTATGACAAAATGTGAGCTAAGGTAGGAGGTGACAAATAATGGCAATTGATAAAAAATATGAATCTGAAATCAAAAAACTTAAACAAGAAGTTTATGAAGCTAATATGTTATTAGTTAAATATGGACTTGTTATTCATACTTGAGGTAATGTATCAGGTATAACACAAGACCGTAAATATATGGTTATTAAACCAAGTGGTGTTTCTTATGACACACTTAAAGCTGATGACATGGTCATAACTGATTTAGATAACAATGTATTTGATTCTAAATATCGCCCTTCTTCAGACACACCTACTCATACCTTACTTTATAAAGAAAATCCTGAAATAAAAGGTATTGTTCATACTCACAGTCCTCATGCAGTAGCTTTTGCACAAGCAGGTAAAGATATTCCTTGCTTTGGTACAACACATGCAGATAATTTTTATGGACCAGTGCCATGTGCTAGAGCTCTAACTCCAAAAGAAATTGATGGAGAATATGAACACAATACAGGACTTGTAATAATAGAAACATTTAAAAAACGCAAGATTGACTGAAAAGCTTGTTCCGCAACATTAGTTAAAGAACATGGGCCTTTTGCTTGATCATTCAAATCTCCTAAAGATGCTGTTGACTTAGCCTTAACACTTGAACAAGTGGCAAAAATGGCTATTAATACCTTAATAGTTTCAAATAATAGTGCTCCAACTGCGCAAGAAGCATTGATTGAAAAGCACTATAACCGTAAGCACGGAAAGAATGCTTATTATGGTCAAAATAAATAATATAGACTACTTCATTTTTGATTTAGATGGTACACTCTATGATGATAATAAAAACATCTTAGAATCAACCAAAAACACTTTAAAACAGCTTCAAAAGGAAAATAAAAATATAATAATTGCTACTGGTAGACCTTATTATATGAACTATAAATTAATTAAGGATTTAGATATTAAACTACCAATAATAGCAGTTAATGGGGCTATGATTTATGATCCTATCACTAAAGAAGTGCCTTATTTTCAAGGACTAGACAAAAAAGTTGTTCAAGAATTAATTAACTTTTTAGAAGAGAATAGAATTGATTTTTTAGCTTACGATATTAATGGTATGAAAGGTAATAATTTCTCGAACCCTTATTGATTCGAATTTATGATCTATAAGAATGTAGTGGAAACTAATCCTTATCGTTGAGATTATCAAGAAGGAAGCTTAAAAGAAACTTGTAACCAATTTAACTTTGCTAAAATATTAATCATCACTCAAAACGTTCCACAAGAGAAGATAGACTCCTTACTTACAATTGCAAGCAAATTTCCAAAACAAATATATACGGTATCTTCTCAAAAAGGTGTTATTGACATAATGCCAATAGGAATATCAAAAGGTGAAACATTAAAAAAATTAGCTAAGATTAAAAATATTGATTTATCAAAAGCAATGGCTTTTGGTGATGCTGATAATGACATAAGTATGTTAAAATCTGTTAAAATTTCAGTTGCAATGGGTAATGCAACAGAGAATTTAAAGAAAGTTGCAACTTATGTAACAGATACAAATAATAATGATGGAATTGCCAAAATTATTAAAGAATTAGAGTTAGTAAATGACTAAAATTAAAGTTTATAAAGTTAACATAATCGAAAATAGATACTGATATTGTCCTTCACTTTTTACATTCAGTAGAAGGTTATGAGCTTCAAGACCATTTGCTACTCTTGAAGAATTAATGAGACATTTAGAAATTAAATATAATCCAGGGTATTTTAATTTCAATGGTGATCTAAGATTTAAAGCATTCAATGAGTTACAACGAGTACATAAAGTAGGTATCTCAATTAATCCTTCAGCGGTTAAAGATAAAGACAATTATTTCAAGTTTCATATTAGTGAAAATGTTGAAGTAGTGTTAGATGATTTATCACTAAAATTAATTAGTAAAGGTAGATCGTTTGCCTGTCCAATGCATTTCTTTGATGATTTATACTTAGAATATTTTGATGAGAAAAAAGTAACTAAAAATCAAAAATTGAGATTAACTTGAAGAAAGTATTTCTTCAATATTGAAGTTGTTGAAAAAGAACAAACAGAATAATAATAAAAGAATGAACTTTTGCTTAAAAACAAGGGTTCATTTTTTGATATTATTTTGTTCTAATTAGCCTATAAAAAAATGTAATAAGTAAAAATTGCATTCAATTTTTGTTTAAATATTAAAAACAAAAATTGAATTATTATCGCTTGCTTGAGGGTCTATTTGAGTGTCTTTTTTGACCTTAACTTCTTTTAGGTTTGTACATCCTTTAAATGTATTTGCTTGTATTTGATTCACGTTGGTATTTGTTAAGTCTAATGTTTCTAATGATTCGCACTGTTTAAAGGCATTAGTTTTAATTATTGTTGTGTTATTTGGGATATGAACGGTTTTTAAATTAAAACACATTTCAAAAGCACCTTCAGTGTTTAAATTTACAGTATCTGGAATGTTTATTTCTTCTATTGAATTACAATGGCTAAAAGTTTCTTTTCCAATTATTTGAAGTTGACTGTTGAAGATAATTTTTGTTAGACTTCTACAACCATAAAAAACACCATCACCTAATTTAATCAAATTTGCGGGGAATGTAATTTCAGTTAATGAAACACAATAGGCAAAAACTTTGTTTCCAATAGTTTGTAAAGATTGAGGTAAATGAATTTTAGTTATTTTATCAAAACCATTAAATACTTCATTTTTAATTTTAGTTATACCTTCTGGTAAAGTAATTTCACCCGCGTAGTTTTTATTTTTGCTAAAAGATATTAGTTCATTGTTAACTATTTCAAAATCTTTGGAGTTGTTGTTTGAAGTTTGATTATTACAACTTAATGTGCTTAAAGGTAATAATGGTAAACTTCAAATTAAGTTTGATAAAAGTATTTTCTTTTTCATGTTAGTCCTCCTTTTATCTATATTTTTATTCTACTCTTTTTTGAAATAATAAAAAATGAACTCGCAAATTCGAGTCCACTTTGTAAGTTAAATTATTTAACAAGCATTAATTTGTCAGCACGTTTAGTCATTTTTTCGCCAAGAGCTGTATCAATAATGTGTTTGTGTTCTGATTCATTAAGAATTACAACGATAACATCACTTACTTTATTTTGGTTTGATAAGTATTCAACATCTACTGTACATAACAAGTCACCAGCTTTAACTTTTTTGCCTTGTTTTAATTTGGTTTCGAAACCTTCGCCATTTAAAGCAACAGTATCAACACCAATGTGAATAAGTACTTTAATACCTTCTTCTGTTGTAATACCATAAGCATGTTTTGTAGGGAATACTGTTTCAAGAGTACCTGAAACTGGAGCTCAAATTGAAGCTTTCTTTTCAGCTTTAAGACGAATTGCAATACCATCACCTAAGTATTTATTTGCAAATGTTTCGTCAGGTACTTGACTAAGAGGAATTACATCTCCAACTGCTGGAGCAAAGAATTCAACTGGACGTTCTAAGTCGTTAGATTCGTCAACTCATTTAGTTTCTGTCATTGATGATGTTTCAACTTGAGGTTCAGGTGTAACTGCTGGAGTTTCTTGAACAGTAGGTTGTTCTTGTGTTGGAGCTTCAACTACTACAACTGGTTGTGTTGGTTCGCTTTTTGCTTCAACAACAATGTCATCTAATTCACCTGAAGCTAAAATTTTTCTAATGTTAGCATTCATTTGTTCAGCAACAGGGCCAACAATAACTTGAACGTGTTTATCACCTTCTCATTTAAGTGCAACTGCACCTGCTGCCTTACATTCAACTTCATTTACTAAGTTTCTATCGTTAATATCATATCTTAATCTTGAAGCACAGTTATTTGTGTTAACGATGTTTTTTGGACCGCCTAAAGCAAGAATCATTGCATATTCTCTTGCGTTTCTTGCTGAAGTATCTTTACTTGAAGAATTTTCATCATTTCCATCTTGTTTAGCTTGGAAGTCTTTTTTGGTGAATAATCTAACTGTTTCACCACGACCTGGGGTTGCCAAGTTGAATTTCTTGATGAAGAAGTAAAAACCACCAAGATAAATAAAGAAGTAAGGAACTCCGACTGCTAATGCTCATCAGAAGTTTGTACCTTTCATCACAGGAATAATGCCATAGATGATTAAGTCCATAATTCCACCTGAGAATGACATACCAATGTGAACGCCAAGAACGTTAGCTAACAAGAATGATAATGCACAGAAGAATGCATGGAATCCCCAAAACAATCATGGTGCTAAGAATAAGAATGTAAATTCAATAGGTTCTGTAACACCAGTAATAACTGAAGTAAGAGCTGCAGGAATAACTGTACCAGCTGCCACTTTTCTATTTTCTTTAGGAGCCGCTAAAATCATAGCAACAGCTGCACCCGGTAATCCTAGAATCATGAATGAGTATTTGCCTTGCATGAATCTACCTAATTTAATGCCTAATTCACTAGCAACAAATTTATATAAAGGTAATGTATGAACTTCATGTGCTGGTGTAGCATCTTTTCATGTTATAGTGTTGAAATTTAATTTCATTACACCAATAGCAGCATATGAGTCACCCATTCATTCATTGTGTTTAGAAGCCATCAAAGCTTTTCAACCATTGTACGCAGTTAATGCAGTAGCATCTCCTGAAAAAGTATTTCCTCTTGCTTCTCAATCTTCAAGCGCTTTAAGAATATCACCGCCTGCATTTGTGTATCAAAGAGGTGCATAAAATGCGTGGTGTAAACCAAATGGAATTAATGATCTTTCAATATAACCAAAAACAAATGATTCAATTCCATAAGGAACATGGCCAAGACTATTACCAAAGTAACTAAGACCAATACCAACTCATGGTCATATAAGGAGGAATAAGAATGCTAATGGAATCATAGCAACTATTGTTATTAATGAAACAAAACGTTTTCCTCCAAAGAATGAAATAACAGTTGGAAGTTTAATTGTGTGTAATTTGTTGTATAAGTATGAAACTATTAAACCAACAAAAATACCACCGAAAACTGAAGTTTGAAGAGTTTTGAATCCTAATGAAGAACCAACGACTTGTTTAACTTCAGCACCAAATTTACCTGTCATTCAAAGAATGTTATATCCTTTTAATGTTTCATAGTAAAGTTGTCCTGTTTTATCATCTTTAAGTATTGTTTTACCTATTAATTCACTAGCTTGTTTAGTGCCATCAATATTAGTTTTTTCTATAACACTTTGAATAAATGGAGCTTGTATTGCTGAGAATACTAAGTATCCAACTATTGCGGCGAAGACACCGACTCCGGCTTCGTCGGTAAATGCCACAACAAAGGCAGCGGCAAACAATAGCGGCATTGCTCCAAATATTGGGTCACCTAATAACTTAATAAAGTCACCAATTACAATACCAGCTTTAGCAGCAGATGATAATGGATTGTTGTTAAGAACAATCGCTGATTGACTTGATATTGTTGCACCAACCCCTAGAAATAGACCAGCTATAGACATAACGGAAATTGGTAACATGAAAGCACCAGAGATTTTACTTAGAATCTTACGAAAATTTCCCTTACGGCTATTGTCTTTTGGTTTAGATTTTTTGTTTTCTATACCTGTAGTTTGTGCTGTAGTCATTTTTCTCCTTTCATATACATTTAATAAAATTACCCTAAAATCTAATATTAATAATAAATATTAAGGTTCTTAAATTATAAAAAGAAAATCAATAAAAATAATTTTCAAAATTAACTATGTGAAAAAAATAAGGAATTTATTCCATACTAATTTTTTAATCATAATCTTTTTAAAAAATTGAAAAACTTTAAAAAAATATTTTACTAAAAAGCCTATAAAATAGATAAATTTATCAATTATTAAAAAAGAGGTTAATGTAATTGAAATTTGATTATAAAATGAAAAAATTTTTAGAGAGGTAAAAAAACTTTCACATAAAGCAAAAATTTTAAATAAAAAAAGGACTTTTTTGAGCCCTTTTTTCACATTGAAGATTAATATAATATTTTGAAATAAAATGAGAAAAGCATCCGCGCACAGCACTTTTCAATTATCTAAAGAAAGGAGTCTATATACTCTTAAGCAACTATCCCTATGGGTATAGTAATTGTCCATCTAAAGGGGACACATCGACATCATTATATACCATTATTGGGATTAAGTCAAGAAAAAAATATACAAAATGGTTGGTACAATTTTCTTTAAAATTTTGAAGATATTAATTTATGCTTTTACTTTACAAAAATTCTTTTTTGTATAAAATAAAGTTAAATTTATATATTCATATAGTAAAGGGGTTAATATGAAAATTTTTAGAATTAAGGAAACTTCACCTTATGTTACTTTAGCTTTAGAAAATATTATTATGCATGATCATGATTTAAAAGGTGATGTACTTTTAATTTATCAACATGATAATGCTATTATTATTGGTAACAATCAAAATGCATATGAAGAAATTAACCGTTCTTTTGTAAAAGAAAATAATATTAAATTAGCTCGTAGAATGTCTGGCGGTGGAGCTGTGTATCATGATTTAGGCAATATTAATTTTAGTTTCATAACTGACTATGATAAAAAAGGCGGTTATGAAAGATTTCTAACTCCAATTATTGCTTTTCTTAAAAGCTTAGGTCTAGATGCTCAATTTAAAGGTCGCAATGATGTTGTGGTTAATGGCTGCAAAATAAGTGGTAACGCTCAATATATTAGTAATAATCGAATAGTAAGCCATGGAACATTATTATTTGATGTTGATTTAACAAAATTATCGAAAGCACTTAATCCTGCAAGAATTAAGTATGAATCTAAAGGCATTCAATCAGTTAGATCTAGAGTAGTTAATATTTATGATGAATTATTAAACAAAATGACAGCTGAAGAATTTATTAGTCGTCTAATTAATTATTTTGTTAAGAATTTTAAAGGCGAATTTATTGATATTGATTATGCAAAATATCAAGAACAATTAAACTCTTTACAACAAAAATTTGCTTCTGAAGAATGAATATATAATAAAGCAGCAACTTTTAAATATAAAAATGGAGCTAAATTTCCTGGTGGAATTTTAGTAGTTAAAGGTAATATTGAAAAAGGTACTATTAAACAATTAGTTTTTGAAGGTGACTTTTTAAGCAAAAAAGATATTCATGAAATTGAACCCTTATTTAGTAATGTAGCTTTAAATGAACAAAGCATTCTAGAAGTTTTAAATCAAATTAAAAACTTGAATGAATATTTTGGAACTGTTACTAAGGAAGAAATTGTTAGCTTGTTAATTGGTTAGGAGGCTAAAATGTATAAAAGACAATCAATTGAACTTTTAGGCGAAACAATTTATTATGAAAGAATAGATTGTTTTCAAAAAGAAAAAAGACCAGCAACTGTTTTACTTGTGCACGGTTTTAATTCTGATTCAAATACCTTTACTAGATTTATTGATTTTGCTGAAGTAGTGGCCGAAAATACACCCGATAATGACGAATTAAGAGAAGAATATAGAAACTTTCATCGTTTCAATTTATATGCTTTAGATTTGCCTGGTTGTGGAAAATCTGGAAAAAATAGTCAAATAACACTTGATTTATATAACAAAATAGTAGTTGAATTTATTAACACAGTTTTAAAAGACCACGAAGTAATTTTAATTTCGCATTCAATGGGTTCAGTACCTTGTTTGTATGCTAAAAAAGAATGCAAAAACGTAACTAAGTTATTTATGGTAGCTCCTTTAAATTATGCTTTAGTTGACGATAAGCAAAAACTAATTAATCTTAAAAAATGATTGATTCCTGCTAATGTTGAAGATGCTAGAAATTCATTAAAAGCATTGGTTTATAATCCTGAAGATTATTATTTAAGAAACTTGGATTATAATGCTGAAAAATTAGCGAGCAAATATAATGAAAACAAAGAAACTTTTTCTTATCTATTAAATGAACAAATTTTAAATAAAGATTATTTATTAAAACATGTAAAGCCTTTATTTGCAAATCCAAATGGTTGTGTAATTATTTATGGTGAAAATGATGAATTTGTGACACCAAAAGAAATTAAAAAAATAGAAGAAGATTATGGTTATAAAATTGTGCAATTAAATGAATGCGGACATGCTGCATTTTATCAAAAATTAAATACCATTTATTGAATAGTTCAAAAAGAATTAAATTATAAATAAAAATAGAACTTTTTTGCAAACTGTTAATTTCAAAAGTGAGACCCCATGTTTTAGAGATCTCACTTTTATAATTTACATTATTTATATTCATTGATAATTATTGAATGTTTAGTAAAATAAGTAATATATATTATATTAAAAGGAAAAATATGAATAGGCAAAAAACTTTATATCAACCTAAAATTTCAATTTTAATTCCCTTGCATAATAGTGCTAAATATTTTAATTCCACTATTAAAACTATTCTTAATCAAAGTTATGAAAATTTAGAAATTTTATTCCATGATGATGCCTCTAATGATGAAACAAGAAAAATCTTAGAAGATTTGACCATTAAAGATTCTAGAGTTAAATATATCTTTTCTGATACTAATATTGGTTCAGGATATTCAAGAAACAAATTAATTGAAGCTTCTACCGGAGATTTCATTTTTTTCATCGATGATGATGATGAATTTTTAAGTCCTAAAATAATAGAACGTTGTGTTAAATATTTAAAGCCTAATTTAGAAATACTTTCAACACAATTTAAATATAAATTTGATGATTTAGGTATTAAAATACCTATCAATAATATTGTTAAAACATTGAAAATCAATCCTTCACCAATGCATTATTATTTGAATACCACAACTTTTGCTTGAGGACATTTCTTCAACAAAAAATTTTTATATGATATTGATGTTAATTTTAGCAAAGTTAAAAAATATGAAGATATTGCAGAAATGGGAAACATTTTTTCCAAATGTAAGAATTTTAAAAGAACTTTGATTTATTCTATAATTTATCATAGAAAAACCAATTCATTAACTTCATTTGATAAAAACTATATCAATAAAATTGAATGTATCAACCAAGCCTACAAATTTAATTTAGAAAACATAGTATCAAATTTAAAAAATGATATAAGTTTTAAAATTCTTAAAAAAACTGTAAATTCCTTATTTATGGAACATTTAAGTTTGATTAGTGTTTACTATATTAATTTAAAAAATCGAAATTTAAAAAAAGAATTTAAAAAATTCATCAAAGATATTTATCCTTCAATTTTAGAAATATATAATGGTTTTGATGTAAAAATCAAATATCGAAGAATTTCTGATTTACCAGTTTTTGTTACTAAATTTTTATATAAAAAACCAAAAGTTAAAAAATAGAAAGGAGAAATAATGACTTATTTATTAATTGGTGGAGCAGGTTATATCGGAAGCCATGTAGCTGAAGTTATAAATAAAGAAAATCAAAATAAAGTAATTATTTATGATAATCTTTCAACAGGATTTAAAGAATTTATTGAAGAAAAAAGTAAATTCATTCATGGAGATATTTTAGATATAAAAACATTAGATTTAACTTTTAAAAAAAATAAAATAGATGTAGTAATTTATTTAGCAGGTTTAATTAAAGTTGGTGAAAGTGTACAAAAACCACTTGACTATTATGAAACAAATATTCAAGGCTTAGTAAATGTTTTAAAAACAATGAAGAAGTATAATGTTAATAATTTGGTATTTTCTTCATCAGCAGCAGTATATGGTAATAACTCATTACACGATGGTTATTTTTTTGAAGAAGACCCAAAAGAACCATGTAGTCCTTATGGTAGAACCAAATATTTTGGAGAACAAATTATCAAAGACTTTGCTAATACAAATAGTGATTTCAAATATACTTTTTTAAGATACTTCAATGTTGCCGGTGCTTCAAAAAGTAAAAGAATTGGTTATTTAACCAAAAATGGTGTTGCACCGACTCATCTTATACCTGCTATTAGTTATTATGCTTTTGGTTTAATTCCCGAATTCAAAATTTTTGGTAATGACTATAACACACCTGATGGGACATGCATAAGAGATTATGTATATGTATATGAATTAGCAGAATTACATTTATTAACTGCAAAAAAAATGATTAAAGAAAATACTTCATTATATTACAACATTGGTAGCTCAAAAGGCTTTTCAAATCTTGAAGTTGTAAATGCTTTTGAAAAAAATTTAAGCAAGAAATTAAATATTGAATATGGTCTAAGAAGAGCAGGAGACCCTGATAAATTAATAGCTTCAAATAAAAAAATATGTAAAGAATTAAATTATGAAGTAAAAACTAATTTAGATGAAATAATTTCTAGTGAGATTGAATTTAGAAAAGAACATTTATAAAATGTGTGAAAGTTATAAAAGCGAGACCCACCATTTTAGTGAATCTCACTTTTTTTGTTTTGTCCTTGTATAATATAAATTGCATCAAAAGAATTAAAAATAAAAATAATGAATTTATAACTGAATATATACATGTTTTTGTCGAAAAATACGATCTTTTAACATTTATGAATTCGTTAAATAGAAATAAATTACATCCCAATAATGTAAGAATTGAAACAGTTATTAAAGCACTAGAAATGCTTAAAGAATTAAATAACTTTAAGAATATTCATAAATCTTTAAATTTAAAACGCGAAACATTTCAAAAACTATTTGATGACTTATCAGATGATAGAAAAATGATTTCCAAAACCGATAAATTTTTTAAAGGTTTGACGTGCCCACATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTTCAGTTTTAAATAAATACAGAAATTGTAGAAGAATAATAGTTAATCAAAAAATTAAAAATAAACACAAAGATTGAATTAAATATATAAATCAAAATTCAAATGTATATGATAGATTATGCAATGAAAATAGCAAAAATTCTTTAAGAACTTTTTGCCAAAAATCAGTTCGAACATTAATTGATGAAGCTAAAGAAAAATGCTTAAAAATTAAGTTTCCACACTTCACTACAATATACAAAAATATAAACATTTTAATATTTGATATAAATATCTCAATTTTGCCCAATAAGTCTGTAGGAAAGGGAGAAAGGCATAAAAAAGACCCTTCAATTCCAAAGAAAAGAAGAGTCGGATTACATATAAGTTTAAGACCAGAAATTACAACAATGTTCGATTTTGAATTTGATACAGTACATGGCAAAAGCGATGATTTATATCATATTGCTGTTTTAATAAATAGAGCAACACGAAATTTTATATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAATACATATGATTGATGAACATGAATTAAATATTAATACTTTAACCATTGACAATGGTTCAGAAAATTACACTTTAGATCAGATCAACACTATTAAGCAAATATATCATTGCGATGCTTTCAATTCAAGTCAAAAAGGTAGAATTGAAAATTGTAATAAAATGATAAGAAAATATAGACCAAAATCAATTACTTTTGATAATTTAACACATGAATGAATTGAAATAATTAACGAAAAAATAAATAATTTTGTAAGACATGAAGTAGATGATTTTCCATTTGCTATGTCAGCAAATCAATTTGAAAAATTTATTCAAGAAAACAAACATTTATACCCATCATTTTATACAAATTTATATGCTTAATTAAATATTTTAAAAAATAAAACTACCTAACTCAGCTGAGTTATTTTTCCTATTGCTTTATTTCGAATTATTTTTATTTATTAACAAAAATAGCAAAAAAACTTTTATAATATCTTTAGCAATTTGTGTTTAACGGACAAATTATTTTAAAAATCCCTAATGGTATGTCTCACTTTTATAAAAATTTTAATAAGATTAAATTAATATTTTACTTCTTAATTTTTGATATAATTTGAAAATTATCTTTTGAATTATTTATATTATATTTTAAATATAATTAAAGAGAAAAATAATAAAATACAATTTACATTTATATACTTAAAACAATATTTTGAAATATTATTTTAGAAATTTGTAGTAAATTTAACAATTAAAAAATTGGATTTTATGTAGTAAGGAGTGGTAATGGTATATCAAAATGTAGTTTATATAGTTATGCCAATTATTGTAGCGATTTTTTTAATTTTTTCATTTCAATATTGATTTGTTTTATTTTTTGGTTTATTTGCTAAGCCAGTGAAATTTAAGACTCAAACTCAAAAGCTAAAATATGGTATAGTCATTTGTGCTAGAAATGAAGAAAAAGTTATTGCTGAGTTAGTAAAAAGCATAAAAAATTCTCAATATGATCAAGATAAACTCCAAACATTTGTGATAGCACATAATTGTACAGATAAAACAGCGGAAAAAGCAAGAAATGCTGGTGCTATTGTTTATGAATATTCTAATTCTAGCGAAAGAACTAAGGGTTATGCATTAAAAAAAATATTTGAATTCATAGAACAAGACTATGGTATACAAAGTTTTGATGGATATCATATATTTGATGCGGACAATATAGTTGATAGTCTATATTTTGAAAAAATGAATGATGCTTTTTTACACTATGATAAAAAAAATGTAATTACTTCATTTAGAAATACTAAAAATTTTGGTAAAAGCATTCAAACAATTAATTATGGACTTATGTTTATGTTCCATACAACCATTGAATCAAACGCTAGAATGAAGTTTAAGATGTCTTCAAAAATTTTGGGTTCTGGTTTTTTAATTAGCAATGAAATGGTTAAAGATGGTTGAAATATAACTATTCCATCCGATGATTCAGACTTTACGGTTGAACAAATTTCAGAAGGAAAAAATGTTAAATATTGTGATGAAGCAATGTTTTATGATGAACAACCTACTAAATTTAAAGATATGTGACGTCAAAGATTGAGATGAGTTTTTGGAAATAGAATTGTTTGAAGAAAAAGATTTAAATCTTTGATAAAAAATATATTTTCATCTAAAAAAGAGGATGATAAAAAAATATCAAAGTCTTCTTCTATTGATATTTTATTTATGATATCACCAATTAGTTTTATAGGTTTTTTAATTTTACTTTTAAATATTTTGTTAGTTTCACTTGGACCTATTTTTGGAGCTAATGCTACTCAAATGTGAATTGATTGAGCAATATCTTTCTCTATAAGTCATGCAATAATATACTTAATATTATTTGTAATTGGTATAATTACATACGCAAGAGGTAGAAAAAGAGTTCAGCGTGTTCCATGGTGAAAAATGATAATATCATTATTTATTTGACCTTTGTTTTTAGCGTTATACTTTCCTCTTCAAATTGTTGCTATATTTAAAAATATCAATAAATTTAATTGAACACAAATTGAACACAACAAAAACATTTAGTATTAAGAACATTGATAGGAAAAATTTATTTATGAAAATAACTATTATTTCAGGTGGATCAGGTAATGATGCACTAGTAAAAGGATTAAAAGCTTTTTACAAAGATAAAAATTTAAGCATTAAAGTTATTGTAAATGCTTATGATAATGGAAAATCTACTGGTGTATGTAGAAAAGTTACTGATACTTTAGGAGTATCAGATATAAGAAAAAATCACTCAAGAATGTATGTGAGTGAATATGGCGATAAAGTGGATAAAAACCTTTTAGAGTTTTATGAAAAAAGATTTGATTTCACTAAAAATAAAGAAGAATTAGAAATTTCTAAACTTTTAGATAAATGAAATTTAAAAGAATATAAAAAATATGTTTCTAACTTTTTCAAAAACCCTAAGGCCAATGAATTTAATTATAAAGACTTTAGTGTTTCAAATATAGTATATTCTCAAATGTATAAAGAATTAGGTTATGAAAAAACAAATAAACATTTTTGCGATAAAATGGGAATTGATGATTTCGTTGTTTTAAATTCATTTGATAATATCTTTATCAAGGCATTAAAGGAATCTGGAACAATAATAGATGATGAAGGAAAAACTGTCTTTTGAAACAATAAAAACGATAGAATAATTTCCACCATATATGAAGGAAAAATAAAATATGGTTTAAATCCTAAAGCCATTGAACTTATTGATAATTCGGATTTAATTATTATATCTACTGGTACTTTTTGATCGTCAATTCAACCTACAATTGAATATATGAATTTTTATGATTATATCAATAAGGCAAAATGTCAAAAAATTTGAATCATTAATAATGAAGATGATGGTGATTCATTTGGAGTTACTAATTTAGATTTTATCCATTATATGGAAAGAACCGGTCTTGACTTATCAAATGTAAAAATTTTACTTAATAAAAGTGCAAAAGAAACCTTAAAATTAACCATACCAAACTATAATTTCATAGTCAAAGATATGGGAAATATTAAGGGTAAACATGAACCAGAAAAAATGGTTAAATCTGTTATTGATACCTATTATGATAAATAATTAAGGAGATAGATGAAATTCGGAATTATACTATCTGCTGGTAATCAAACTAGATTTAAAAGCGCAACTCCTAAAGCCTTGGTTAATGTAAATGGAAAAATTTTACTTGAGGAAAATATTTTGCGAATGTCAAAATATTGTGATCATATTTTTACGATATGTTCTTCCCAAAATGAGCATTTTTTTGAAAAATATAATCATATTGCTATCTCATCAGGTAAGGGTAGTGGAGATGCTGTACTAAAAGCATTATTGGAAATCAAACAAAAATATGGTTTTTCTTCAAATGATACTTGTTTTATATTATGAGGAGATTGTTTAATTGAAGACTTAATTTATAAAAAAGTTGTAGAAAATTATAAAAAAGTTGGTATTGTTCCATGTGTATTTGAAGAAAAACCCTACGTTCAAATATTTCAAAAACAAGATAATAAAATTGATGTTTTATTTTCTAAATTTGGAGAAAATGCATCATCTGGTTTTCATGATTTAAGTATTTTTTATTTTAATTGTGAGGAAATATTAAAATATCTTTTAGATTTTCAAAATAAAATAATAGGTAGGGATGGAAATTATGTTCATAAACATGGAAATGAAATGGAATTTTTAGATGTTTTTAATGAAACAGATATAAAAGCTCAAATATTAGAAATCAAAAATTATCAAGATATGTCATTTAACACTATAGAACAATTAAAAAATATAAAATAGTTTTTACAAAAACATCCCGTAACTTTCGGGATGTTGCTACATTGTAATTTATGAGTTTATACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATCTTTTGTCCGCTAGGACAAATAAAAGTGAGGGAATTAACCCTCCCTAAAATATTTGGCAACTTGTCGCTCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTGAAAAACAAATTTTTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAAATAAATATCCAAAAATTTCTCTTGGATGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGAAAAGAAGCATATGGACCAGCTTTATATATAAAAATTTTTAGTTTATATTTAATCAAAAATAATGTTAAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCACAAATACAAATTTAAATTTCGTGGATTTTTATTAGGAATTTTTACCAAAAATCCATATCTAAAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTGCCTTTGCTCTTACCTACAATAAGGTCTATTTCCCAATGGCCAAATTCATTTCTTTCATTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCGCCAAACCAATCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTGACAGTTCACAATTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATCTAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGTTATACTATAATTAGTTGTTCAAAGTAAGTATTCAAGAATAATGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCCTTGGGTGACTTTTTTTATAAAAAAACTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAGTGAGATCTCTAAAACCTAGGGGTCTCACTTTTGGAATTAACACCGAGATCTAACTAAAATGTTTTGGTTTCTTATTGAAAGATTTTTATATATAATTACATTTATGATAGAAATAGTTAATTTATCAAAAAAAGATAAAAAAAATCGATTGATTTTAAACAACTTAAATTTCAATATTCATGAAAATGAATTGCATGTTTTTTTGGGCAAAAATGGTGCTGGAAAAACAGTTACTTTAGGAATAATTTGTGGATTAGATTCAGAATTTTCTGGAAAAATTTTAAAAGATAATGTAGATATTTACAAATCAAATAAAACTAATAATTATTTGTTTGTTCCAACAGATATTAGATTTCCAAATCATTTAAAAGTTATTGATTTAATTAAATTTTATGTGAATGAAATTTTTGAATTAGAAACAAATGAATTATTTATAAATGAAACATTAAAAGAATTCCATTTAGATGAAAGAAAGGACTATCTTCCTAAAAAATTATCTTCTGGAGAAAAAAGAAAATTAATGCTATTAATATCTAAATTAGTAAAACTAAAATATTTATTCTTAGATGAACCAGAAGCTAATCTAGATCCTTATTCAAGAAATGAATTATTTAAACTTTTGAATATGTTAAAAAAAGAAGGAACTTCAATTTTCACATCATCTCACTTAATATCTGAAGAATTGCAAGATATAGATAGAATAACAATTTTGCATAACAAAACTATCGTTCTAAGTGAAAAAGTAAAATCAGCAAAAAATTTAAAAATTATTTTTGATAATTTTAAGAGAGATGGTTATGACTAAAAAAATACTATCTCTAACTTTTAAGTTTAATAAGAAAAACAAATGAGTATATATTGGTTTATCACTTTCTTTTATAATCAGTTTTATATTTTGTTCTTTGTTCTTATTTGATAATTATCTTTTTTATAATTATTTTTGAATGATTTTTCTATTGGATTTTATTATAGTTTGTGTGTCAACATTATTTATTCTGCATTTAACATTTGTCTCAAACCAAAAATCGATATATGAAAACACATTAAATTGTAGATATTTTAATAAACCTAAAATTGTTTTAGCTTGGTCAATATATTCATTTATTTTATTATCCGCATTTTCATTATTAAAGTCTTTTGCAATAAGTTCATTGCATTTATATAGAGACACGAATATAAAAAGTTTTCTACTTTATTTTATAGGCTATTTTCTAAGTAATTTATTGATAATTATTTTTATTATTTTAGTTTTTGCAAGTTTTATATTTATAACAAAATCATATAAAAAGTCTCTCGTCATTGCAACTTGTATATTACCTTTTTTTACTGTTGTGTCTTTATCATCAAAAACAATAGAAGCGCGAGCAAATAAAAAGGTAGAAATAAACAAGGATATAATAGTTTCTGATGACAAGTATTACTTTTTATCAGATGATGAGATTAAAAGTAAATATAATTTGAACTTAATTTCATCTATATATTTTTTACCCAAAAATACTATAAATAAGTACTTTGATGAGAATTTCTTGACTTATAAAAATAATTCAGATACAAAAAATTATATATTCTCAACATTCAATTTGTCTATTCTAAAAAATAATAAAAAATTTGACAAAGATAAATTTAGAATTTATCATAACGATGGTGAGTTTTTTATAAAAATGAGTACAAATGAATTGGCTAATTATTTATATAGCAAATTAAAAAAATATTTTGAATCAGGTGGAGTTAATTTAGATAAATTACTTAAAAAAATAAAATCTAATATATGAATAAAAAACAACTTTACTGGAAATGAATACAATTTAGTAATGCATTTACTTGGCAAAGATGATTTTAATTTTCAATACTTGCAAAGAGACTGAAATGATAATTTATGAAATGCTGCAAAATTAAAGTCAAATTTAAACAGTATGTTCAAAATTAATTTCTTTGATTTTTTAAATCATTTATACAGATCAAATCAAACAAAATTTAACACCACAATTAACATGGCTGAATTTTATATTGTTAAAGATAATAATTTAATAAAATACTTAGATTCAAAAGAAAAATGAAAAAATCTTGTTTATAGTTCTTCAAATCATTTGTTCATTATTAAACAAACAATTGATAAAATGAATGAACTTGTATCTAATTTAGGAAATTATCAAGCGTTTAAATTTGAAGAAAACGTAAATCCCAAAAACTTTTATGACAATACAAAAATAATAAAACTAAATAATTTGACATATGACCTGATAAACTTATTTTTAATAACATTTGTTATTTTAACACTCAGCATTTTGCCAATAATATTTACTTTTAAAAGGAGAAAACATGAATAAGAAATTAAAAATTAATTTACTATCTATTTCAACAATAATGATGGGGGCATCATTTGTTGTCGCATCATCTTGTGGTTCTAAAGAACAAGAGCAAGAAAAAGTTTTAAGTGATAATGAATATGTTGAGAAACACTCTATAACAAGAGACACAAATATTAAGGAAACAAGATATATTAGTCAAAATGTAAACAAAGTTTTTGACAACCACATACAATTAGAAAATTTTGTTAAAAATAATATAAAATCTGTTGATTTGAAATTTTTAAAAAACTCAAAAAAATTTGATGATATAAATAAAAATAATCCACAATTTATGTCCAATGAAGAGCTTTCATATTTTGATTTTAGCAAGTCAACAAGTAACGAAAAAATGCCGATATTTTTAGGACGAAACGGGAAAGTTTTTACTTCATTAGAAAAAGCAAGAGATTCATATTTTAATATAAATCAAGTTTACAAATTAGGCAATATTTATGCAAACAGTAAAGAGGAACTTTACAATTTCATTTTAAATAAATCAGTAAATAATAAAGATTTTTTGATAGAAGTATTAAATGAAATAAAAAGCAAATTGAATTTTAATTCACCTAACGCAACTATTACAGAATTTTTCAAAAGTCCAAGCGGACAAATTACAAGTTTTAAAAAAGAAGACCTTAAAGATGCAGACAAAAAAAGCAGAATTGAAGATTACATTAAAAATGTTGCAAAGAAATATGTAATCTATTATGATGAAGAAAATAATCCTCACTTAAAAGCGGTTGAAGATTATATATTTGATAGAATATCAAACTCTGAATTTATTAAGAGTAAAATCACTAGAATTAATTCTAATAAAGGATATAACAAATATTATGTAGACAATTCCAAAGATGATGAATTTACTTTGTATGGGCCAAGAATTTTAGAGACTCCTTTTGAAAATATTGAAGAAATAACTGATACAACTAAATGAAGTTCAACAAATAATCATTCTAAAGAAGAAATATTTAATTGATTAAAAATACAAAAGATGAACAGTATTGTCACATCACTTTTCAAAATTGTGTATTTTAATGAAAACATAAAGAAAAATGTTGAAATTACACCATTTACACCTATAACAATAAATGTTGAAAAAGATCATTTAAACAATTTCAAAAAATGTACCAAAAACTTTTGTTTTGAAGGCACTAACCTATACGATTATTTATATAACAAATATGATCTTTTAGCAAAAGGAAAAGAATCTAATTTTCTTTCTAGTTTTTATATATTATATGTTGCGGGTTTAAACAAAATTATAGAATATAAAGCAAATCAAAAAGTGGTTGATGAATATAAAACATATTTTAAAATGATCATAAATGACATTAGCAAAATTTTTGCATCATTATTACCAAAATATTTCGTTCATGAAGGAATTAGTGAATCAGAAAACTCAGATAATCAAGTAAATATATCAAAAATTTGAGATATAACAAACGGGGAATTTGACTTAAATTCAGATTCAAATATATTGATTGCTAAATTATTGAAATATAAAATAGCATGCAATTTTTTGGATGTAATTGCAAGTGGCATATTTAATGCAGTATATAGATTTTCAAGTGAAGATTATGATCCAGAATCTCTCATTTTTCCTGAATATGCAATATATGATAATTATGAACATTATTTTGAAATTTATAGTTTAAAGCGATTTAAAAAACCAGTTTTTTCAAAAAATTATGACAAATTTACTAAACAATCATATGATACTAACAACAGCTTACAAGAATGATTTTTAGAAAAGTTAATGTCGACAGTAAAAAACAACTTTGATAATGTGAATGAATTAGTAAAAGATATTCTTCTAAATAATGAAGATTTATTAAACAAAGATAAAAGTTTTTGATTATTAAACTATTATAATAATGAAAAGCTTCAAAATGAAAATTTATTTACTTTTATATGTAGAAAATTTATCACTATTCATCAAAAATATGAACAAATGAAAAAAGATTTGTTGCCAGTAGAAAAAAAATCATTTGCTAAATATTTATTATATGCTTTCATAGAAATAGATAATATAGTTAAAAAAGGTAGTTGGTCAAGCAAAGTTAATCTTTTAGTAGAAACTACAAAATATATTCATTCAATAATAGATGCAAAAGCAGCAGCATTAGAAATAGCTTTAAATGTTGTCGGAGGTCCAGTTTCTGCTTTTGGTGGTTTATTGTCTAACTTTTATGTTAAATTTTACAAAGCATTATTTGGTGAATTTAGCCAATTTAACTATAAATACGTTGTCAATGCAGGAGATAAAGGTAATGTCGATTATATATGGAATGGTGGATTGCGTTATGTTGGTTTATTTCATTGAATAAATGTTGAGTCTGGAATACAACATGCTAAAATATTACCACCTGTAAAAATAATGGACGGTTATACTGAAAGTTTTTATTACTTCAATGGTAAAAAATATAACTTTGTTGATATAGATTTATTAAAAAGAGATGTTGTAAATTACTATTTAAAAGGAAACAACAAAATTATAAACAACTTATTAGAAAAAGATGAAGCTGAAAAGAACATAAAAATTATTTACTCATGAGACAAACAATTAGAACAAAAAATTTCATTTAATGATTATAATAACCCAAAATTTTTAAGAAAATATAATTCTTGAGCTAAAAATGATGGGTTTAATATAATTGAAGCAAATTTTGGTGAACATGAAGATTCTTGATTTTCGTCATTAGTTAATTATAAAAACGGCACACCATTTAGCTTAGCAAAAGAAACAGACGATATAAAAGACATTTTAAATTCAATTAAACAAGCGATCGACATTTTGAATCCTATTTATTATTCAAAAAAACCAATAGAAACAGAAGATGGTTACACCGACAAGCTATTTCCTATTGATCAATTTGATCCAACAAAAAAAGCTTTTGAAGACAAAAAAATACTAGCAAATATTTATAATGAAGATCCATTAAAAAATTGCTTTTTATATTCAGAAATAGGAAACTTTAATATTCAAAGCGATTTGTTAAAAAATAATAGAAACAAATTTATTCCATTTTTAAATTCATTGAATATAAGAACAAAAGAAGTTTCAAATATATCATTTTTTAATAATTCAAATTATCAAGATATGGAATTGTTGCAAACAAAAACACTTTATTATTTTGATTATAAAAACAATAAATACTTCTTTACAAATTTAGACAAAGCGAGAAATTATTTTATCAATGTTATTATTAACCCAAAAATGGAATTATATAATTTAACCAAAAGAACCAAAATTATATTTATGAATAAAGAGTTCTGTACATTGAATGAAGCAGTATCTTGATACAAAAAGAATAATAAAAGAGGCGAAAATAAAAATTAAAAAATTATTTTTATCTTTAATTCCAACAGTTGTTTTGATTCCATCATGCAATAATTCAGCCTCTATGAATTATTATATGCAAAACAATAATTTAAAAAACGCAAGCAAATATGAAATTAATTTAGAAGGACAAAAATACCAATATACTGATATTGAATCATTTATGTCTAATTTAATAAATAGTAATAAAATAAAAGTTGAAAACAAATTAGTAATAGGCAAAAAGAAAAACAATAGTTCAAATAAAATTCTAGATTCTACTATTTTTTATAATTTCGACCCAAATAAACTTAAAAAAGTTTATCAAAATAAATTCCAAAAAACGGTTTATTCAGTTGATGAAGTAATAGAATCGTTTAGAGAAGAAAGCTCATATATTAAAAAATATCTTTTTGCCAATAAAGAATTTGATTCAGAAGAAAAAGTAAAACAGTATATAAAAACTCAACTTAAAAACGGTTTTAATAAATATGTTTATTATTCTAATAATGGGAAAAAATATAATGCTATGAATTGGCAAGATTCATTAGAATTGCTAAAAGATAAATATAATAAAGATAACAATGTGTTTTATCCTATTTTTGATAACAACAATAATGTAATTTATAATAATTTACTTAATTATGAAACAATGAATTCTATTAGTAAAAAATTCTTGAAAAGTTAATGAAGTTTAAAGTAGAAATAAAATTTGGTTTAGATCTCAATGATGAAAAGTCTTATGACTACAATGAAAAGGATTTAGGTTTTAAAGTAAATGTAAAAAATAATAAATGAAATGTTTATGATGGCAATAAATTGCGCAGAACATTCAATTTAAATGAATTTTACAAATTTTATAAAAATGGAATGAAAAATCATAACGGTTTTTGAGATGCAACTTCAAAAAATGTTAAAAATTATTATGACCACGAAAAAGGAAGATTTGAAGATTGAACTTGGATTGTAAAAGCCAACATATTAAATTTATTTGATACAGAAAATGTCGAATTAATTTTCAATGATATAGCGATGAATAAACCGTATGTAGGCACTATTTGATTAATTGATGCAAATTATACGATTCATAAAAAATTAAAAGTCAAAACATTTGTTGGATATGAAAAAGAGCACTATTATTCAAGACCAGTTAATTCATTTGGGCCAAGAATTAAGGCTTATTATGACCAAAAGAGCACATTTGAAGAATTAAACAACATTGACATTAACAATTTCTTTTCTACTTATCAAATTTCAAATAATAATTTTGATTCGTTATCAATTGAAGAAAAAGAGCAAATTGTTACAGTTTATAAAAAATATATAGAATATATTCTTAAAAATGATGAATGTTTAAATCAAATAAAAAATTATAATGTCATAACTTTTGATAGTTATATGAATAAAAATTTGATAATAAATGGTAATGACTATTCAAAAAAAATTGATTTAGACAAAATAGATTGAGATGATTATAAACCAATTAATTTGAATAAAATATGAGAAGAATTTGATTTTAGTTTTTCAAAAATAAATGATATTAAATTAAATAACAAATATATTTATGATAAATTTTATGCAATTACAGGAAATATTGGATTAATAAATGATGATAAAAGTCAAATTTTAACTGCCAACAACCAAAATTGTTCTTTATATGAAAAAAATAAAAATTCTAATATTTCAAATTTGATTTTTCAAAGAAAAACATGTATAGAATTTTTAAACAAAATAATTTCTTCTAAATCATTTCCAAGTGCTTCTGAACCCGAAGACAAACAAAAACTTTTAAAAGAAATTATTGAAGAACATAAGAATAAAAACAAATTAAATAATAGCTTTTTTGAAAATAATTTGCCCTTACTTATTAATGATAAAAACATTGAATTATTTTCTTTCTATTCAAATAATAATGTTGACATCTTATCAAAAAGATATTCAATACCTTCTAACATACTACTTGAGTTTCCAACTTTTTATTCTAAAGATGATTATGAAAATAACATAGCTCTTTATTTTGACGAAAATTGACACCCTGCATATATTTATCGTTTATCTTCATATACCTTAGAAAATAATGAAGAATTTCCAACAAAGGAAATACTTGATGGGAATTTGAGATATTTACTTAGTAATCAATTAGGGTATAAAGAAGATTTAGTTGCATATGATAATAAAGGTGTAGTAGAAATAATTAACAATGACATTTTCAATGTGTTTGTATTGAAATTAGAAGGCAATGAGTTTTCATTCCTGAGTTATAATGATGCAATTTCATTTTTAAAAGATTACATAATAACAAGATCAAAATTAATATAAATTTAAGATGGCAACATCTTATTTTTATATACAGTATATAACACTTTGTTTTTAAAATAACCAAAATAATATAAAATTAAAACACTATGGGATTTTTTGGATATTTAAAAGAAAAACTTTTTGGTTGAAAAAAGAAAAAAGAAGAAAAGAAAGCAGAAAAAGTTGTTGCTAAGCAAAAAGAATTAGAAGAAAAAGCACAATTAGAATTAATAACTTCAAAGAAGCTTGATAAATATGCTACAGGTCTCTCTAAATCTTCTTCTCTAGGTAAACAAATTTTCAACCTTCAAAATAAACATAAAGAAATAAATGAAGAATTCTTCGATGAACTCGAAGAGATTTTAATCATGAGTGATATTAATGCTAAATTAGTTTATGCAATTATCACACACATTAAAAACGAAGTTAGAGCTAGAGGCTTAAAAGATACTAAAGATATAGGTGAATTAGTTGCTGATCAAATGTTTGTTGTTTATACAAACAAAAGTATTGTTGATACAACTTTAAATTATCAAGATGGAAGATTAAATATCTTTATTTTTGTTGGAGTTAATGGTAGTGGTAAAACAACTTCAATTGCTAAAATAGCCTATAAATATATAAAAGAAGGAAAAAAAGTTTTAATTGCTGCGGGCGATACTTTTAGAGCTGGCGCAGTAGACCAATTAGGTGTGTGAGCAGAAAGAGTAGGCGCTCCTATTGTTAAACCTGTTAAAGAAGGAGCTGATCCTTCATCAGTAGTTTATGAAGCTCTTGAAAAAGCACAAAATGAAAAATATGACTTACTATTAATAGATACAGCTGGTAGATTACAAAATAAAGTCAACTTGATGAAAGAACTTGAAAAAATGTATGGTATTATTGCTAGATTCCAAGACAAAGCGCCACACGAATGTTTATTAGTTTTAGATGCAACAACTGGTCAAAATGGAGTTAGCCAAGCTAGAAGTTTTAAAGAAGTTGCTGCCCCAACGGGAATTATATTAACCAAAATGGATGGAACTTCTAAAGGTGGAATTGTACTTTCAATTAAAGACGAATTTGATTTGAATGTTAAATATATTGGACTTGGCGAAGGGCTAGATGATCTTCAAGAATTTGATTTAGATAACTTCATTTATGAAATGACAAAGGACTTAATTGTAAATGAATAAAGATATTAAAGATATAGAAAATAGAGAGCACTTATTAGAACTATTTGATAAATTTAATTCATTTTTAACTCAAACACAAAAACAAGCTTTTCAATTATATTTCATTGAAGATCTTTCTTATTCTGAAGTTGCCAACATTACAGCAACCACCCGTTCTGGAGCTTATGATAGTGTGAATAAAGCAATTAAAAAATTACTAGATATTGAAGCAAAAATGCAATAAATTAATACCATAAAACTGGTATTTTTTTATTATAAAATATGGAATTGTGTGAAATTTTGCCAAAAAAAATATGGAATTGTGTGAAATTTTACCAAAAAAAATATGGAATTGTGTGAAAAACACATTTTTTATATATAATAAAAATGAGGCAAATATGGCTTTATTAAAAAGAAAAATTGATAAATTTTTACAAGATTGAAAATTAAATAAAGAACGAAAACCTCTTATTATAAAAGGAGCTCGTCAAATTGGCAAAACAACATCTATTTTAGATTTTGCAAAAAATAATTATGAAAATGTTGTTTATATAAACTTTATAAGTGATTTAAGATATCAAGGTTGTTTTCAAAAAGGTTTTGATGTTGAGACAATTGTTAAAGAACTTAGTTTTGTAAATCCAAATTTTCAATTTGTAAAGAATAAAACAATAATAATTTTAGATGAAATACAAGCATATCCGGATGCTTCAACATGTCTAAAATTTTTCGCGTTAGATAAAAAATTTGATGTTATATGTTCAGGATCTTTATTAGGTATTCAATATCAATATATTACCAGTAATAGTGCTGGATACAAAAACGAATATACAATGCAATCTTTAGATTTTGAAGAATTCTTATGAGCAAATGGATATAATGAAAAACAAATAAAGCAAATTTTTAACAATATGAAAGAACTTAAACCTTTCAGCGAAAATGAATTAAAAGTGTTATTTGAAAAATTCAATGACTATATTGTTGTCGGAGGAATGCCAGAGATTGTTAAAAATTTCATAAAGCAAAAAAATTTCTCAAATATTTTAAATCAACAAAAAGAATTACTTGAAGTCTATAAGCAAGACATTGGTAAATATGCAGAAAGCTTGGATAGATACAAAATTTTAAAAGTTTATGAAAGTATTTCACCATTTTTAGGTAAAGAAAACAAAAGATTTATGTTTTCAAAAGTTGAACATAATGCAAGGTTTAGAGATTATTTTTCAACAATGGAATGATTAAAAGATAGTGGCTTAATTAATATTTCATACTTATTAAATAATGTAGAATTTCCATTTTTAGCTAATTATGATTCAAACCAATTTAAAATTTATCACCATGACACTTCTTTATTAATAGCTTCTTTAGATAAAGAAACACAAGATGATTTAAGAATTAGAAAAAATTTAAATACTTATAAAGGTGCTATTTATGAAAACATAGTAGCTGAATCATTAAAAAAACAAGAATACGATCTTTTCTATTGAAAGAATCCAAAAAGCACTATTGAAATTGACTTTATAGTTAGAGATATTGATTATATAGTTCCTTTAGAAATTAAATCTAATAATAAAAAAGCAACAAGTCTTAAAAACTTTTTAGAATTATTCAAAAATAAAAATGTTAAATATGGAATTAAATTTGCTAACAGCAACATCGGATTTAAAAATAATATTTATACCTTTCCATACTTTTGTATTTTTATGCTAAGAGATTTTTTAAAAGATTTAGCTTTAAAAAGAAAATAAAAAAGCCATTTAAGGCTTCCAATTTAATTAAGGGTAAAAAATCACAACACTGTAAAAAATTTTAAAAGCTTTGCTTCTATGAATTTGAAGAAGCCCCTTTTTTAAATTGAATTAAAAGAGAGAGGCGTTAAGATTTCTTGCATAGTAAGATTCTTTTCCTATTTTAGAATTTGAATTTCAAAAATAAGATAATGACTAAAAAATATTATTTTATTCTACAATATTACTTTAAATTTTATTTATTTCAATAATTCATATTCGAATAATTTATTTTCTATATTTTTTCTATAGAATTCCCTTAATGAATCTCTAAATATACAAATTTCACTATATAAAAGACCATCATCTTCAAACCTAGTTATAGTTTTTCCAAATTTGTCAATTTCTTTTATATCTTCTTTCAAAAAATCATCTTTTATTTGTTTAAAACCATATTTATATTGTGGAAAAAAGCTTTTTCAATTGCCTGATTCTACATTATTTATAATTTTTTTTCATTTTTTTATTAAATCATTACAAGTTTCATCAAAAAGATTTTTATCAAGCATTTCAGCTGCCTTTCCGTCTAAACAAATTTCATTTTGTTTATCTTCATCTGAAAAACAGCGAGTTTTTTCACTTAGCAATGTTCATAATAAACAATCATTTAAGAATTTTTTATCACTAAATGCTTTTTCTTGGTTATCAGCTGATTTCATAATTGTCAAATTGTCTTTTAAGTAATTTTCACCCACTCAAGCATTTAAACAATTAAGAACTGCGACTTTTAAAATATTTTTGTTCGAAACATATATTTTTCTATTAGATAAGCCATTGTCTGTATTTGTTAAAATGTTTCCATGTGGAACACACATATTGCCTATTGACAATATAGCATAGTAATTTTCCTTATCGCTTTCAACATCAAATTTATTTACTCTATTTCTTATTTGACTTAATGTAATATTATTTTCTAATTCAATAACATCATTTATATTTTTTCCTTCTTTTCATCTTATGATAGGCAATCCTCCTTCTGAAGAAGCATTATATTCTTTTCTATTAGATAAAAAACCTTCTATAAAATCAAAATTTAAATAATTTCATTTTCAATATTTTATTGGAGAAATTAAACAATATTGGTCCTCCTTTCTCATTAATTTTTTTGCGCTATAAATGAATTGAGTGCTCAAATCTCTTATTTGTTGTTTTGAAAATCCATCTTTAATAGCTGTTTCAATAACATAATTATTTGTTACTGCATGACCTTTTCCATGACTGTTTGCAGTCTCATCCCTAAAAGGAGGGTTTTCATAAAAAAGGATTCTTCCATTTGGATTGTTAATTCTAAAATTCTTAATTTGTTCTGATAATCATATATTAAATTCTTTAGAAAGAGCATCACCAGATTGCATTAAATTTTCTTCAACATATAAGTCTGCTAATTTGGCTGTAGAATATGGAGGTTTTAAATATTTTACTTTGTTACCAAAAATATATCCCAGATGCACTCACTCACAGAATTCAAACGTATTTAAAATAGTGTTTTTAAGTATTTCTGTGCTTAGTCCTTTTTCTAAATTACCAGATCCAGCACATCTATCTATTATTAAGTAATTAACACTATCTTTTTTGCATTCATTAACAAAATTATTAAGATATTGTTGCATTTTTTTAGAATACTTATCAGGTGTAAAAAATGCACCACGATCTTTTCTTGATTGACTGCCACCTACGTGGTCTCATTTTTCAGCAATAATTTCTTGCATATCAGTTATAAAAATAGGTTCTACTCAATCAAGCACCTTCTTAGATTTATTTAGTTCTTTTAACTTTGCAGAAAGCTTGTTTATTTTTTCTTGATAGTCTTTTGAATTTTTATCATAACCAGATTTTTTAATACAATAATCAACATCTGATCCTATTACCAAATCTCTTATTAAAAATTTAGGATCTTGATTTTTTGGGTCAGTATTTTTGTAGAAATAATCATTTATATTAGCAAAGTTTATTTTGTTTATTTTTGTTTTTAAAAATACATTTGAACGAACTTTTTTAACTAAATCTTTAGGATCTGTTCAAGTTTGGTCTTTGCCTTCGAAACTTATGGGGTTTGTTTTTGATGGGCTTACATTACTTATTTCTTCACTATAAATATATTCAAGTTGTTCTTTATGCTTATTTAAATCTATTAATCAATATTTTTTTGTTTCAAAAGTAGCAATACATATTGAACTAGGAATTTTTTTAATACCATCTTGATTAAATTTCTTTAAATAAGATAAAGCTTCAGCAAATGCACTATAAAGTGTGCTGCCTGCCCTACCAAAGTTCATTTTGAATTCAAATAGCACTCCTCCGTCTATTCCATCTGTTCCATATTTGATTTTATCCTCATAATATGGATTTTTTAAAACTGGTTCGTTTTCATTTTCATTTATATAATAATCAGTAATAACATAATTTCAAAATGCTTTTTGACAATCTAGTTCAGAATAATCCTTTCAATTAAATTCTTTCTTCTCTCTCTCTCTCTCTCTAATCTATTGAAATTTAATTCTTTTCTCTTGTTGTTATTCATAATTTGTCACTCCTTTTTCCAAAATTATAACATAGGAAAAAAATAAAAAACAACAGGATATAAAAACAAGTTTTAAATTTATAATCATTTTAAAAAAATGAAATTGTGTGAAAATTTGATAAAAATTGTAACGAATTTTGCGAAAAAATATTTATATTATTCGTAATTTATTTACAAAAATATTAAAAAGAAAAATTGATAATTATTTAAAAGAATGAAAAAGTAATAAAAAAATCATTAATTATTAAAGTTGCTTTTCAAACAGAAAAAACTACTTATGTTTTGAATTTTGTTCAAAAAATTATAAGAAAGTAATTTATGTTGATTTCAAGAAAATAAAAAAGCCATTTAAGGCTTCTAATTTAATTACGGGCGAGAAATCACTCTTTTTTATCTTTTTTCGATTATTAACATTTTATGAAAGTTTAGTTCATCACCTTTATTTGCTCTATAAAGTGTTTCACTGATGCAATCAAAAACTAAACATTTTCTTTTTGCGATATATTCTTTATTATCATTAAATAATGTAATTCTAGTTTTTTTATTGTAAAAAACAGCGCAGTCTTCTTTGTAATACCTATTCATTGATGTGGACGAATTTTCATTGTTAATTTTATTTAAATCTTTAAAATTTTTTATTTCAGTTTCTTTAGTATTAACCGATATTTCTAATAACCTTAATTCATCGTCTGAAGTATAAGCAGAGTGTACAAAGTCATTATTAAAATTAATAAAAGATTTATTTTTTAAATTTAATTTTTTAAAACAATTTTTTTCCAAAACATCTGTTTTATAGTTTTTTAAGCCAAAAAGACCATAAGATGAATTGTTTGTTGTTAAAACATACCAAGCTTTAGATGATTTTTCATTTTGTTCATTTTTTAAATAACTTATTTTCATTAATTCATTCTTTTTATTTAAGGTATGCAAAACTATTTGAGGTTCATATTTATCTAATTCAAAAATTTTACTGTTACCAAAATTTGTTAAGTGTTCATTGTAAGAAATTTTGTTATTAGCTTCAAAATTTTCCTCATCTTTAATGTCATAAAGAATTCTATCAATTGATAAACCTTTTTTGCTTACTTCATCAATGTAAAAATGTAATTCTGGAACTTTTCTTCAATTTAAACTTTTAGCCAAAATTGTTCTAATGTATCCAGAAGCATTTTGTAAAGCTTCTAATCCTCTCTTTTCATTTTCGCTAAATGACATATAGATTTTTACATGTGATAAATCACCTGATAATTCGCAATCAACCAATGTAGGATTAATAATATTTGTATTTGTTAAATCATTTGTTATTATTTCTGCTAATAATGTTCTAATTTGTGATTCTCTTCTTAATGTGTTTATACTTTTAGGCATAATATCTCCTTTATTCGCTATGAGCTTTATCTATTAATGATCAATTTTTAATAAGTATTCTTGCTTTTTTAAGTTTACTTGTATTTTCCTTATTATGCTCTAAAACAGCTTCAAATAATAAATCGGTAATTAACATTTGAGCTATTTTTGAACTTGAAGGAACTAACATAGTTGAACTTTGAATTTTGTGATATCTTAATTTAACTGCAATTAATTTATCTTCTTCTTGGCTAGAAGTTAAAACAATAATTGGCGCTTTTTGTTTTTTAGCTTGCTCGATAACAAAGTGAGCTTCCATTGTTTGTAAATTATTTGAGTAAACAATTACAACATCATTTTCAGTAACATGAGCTAAGGATGGAAAGAAAATATGAAAGTCACTATGAGCTATAACAGGTTTACCAATTTTTAATAAATTAGAAACTAAATTCATTGATATTCTTTGACTTGAACCACAACCGTGAATATAAATGTTTTTTGATTTATTAATTATTTCAGCTGCTTTGTTAATATCTTTTATTAGATCTTCATCATAAACATTATCTATTGCATATCTATGACTGGCTGCAATAAATTTATAGTCATTATCTACTTCATTGTCATTTCAAATAGTAGGATAAGTTTTTTCAAATTCTGTTAATTTGTTATTTATTGAAGCAATAAAAGTGCGATAGTCTTTAAAACCATATTTACGAATAAATCTAGAAACTCCCGCATCACTTGATTCAGTATTTTTAGCTAACTTATTTTGTGAAAAGTTAAAATAATTACCTTCAAAATTATTAATAAAATCTCGAATTCTTTTTTCACTTTTAGTTAATTCAACTGTATCATCCATTAATGGACGGCTTATTATTCTTTTGTCATTATTTTTCATAATTGTAATTATATTAAATTTTTTTAAAACTTATATTAAGCATGGTTATAATAAAAAATTTATTTTTTAATTATGCTTACATTATTAGTTTTGATAGTATCTAAAACATTTTATTTTTTTGTTTTGTATTTTTATATTTTATATTAAAATAATAAAGCAATAAGCTTGGGTGTCAAGTATTTGCACTTGACAGCAAGTTAAGTTATTGATAAAAAGAAAAATAAGGAGAAAAACCACAATGGTTAAAATTAGATTAAAGAGATTAGGAAGCAAATTTAATGCTTGCTACAAAATTGTGGCTGCTGACTCACACGCTCCTAGAGATGGGAAATATATTGAAGCATTAGGCCAATATAATCCACACTCAAAGGAATTTGTTTTAAATGAAGAAGCAACACAAAGATGAATTGACAATGGTGCTCAATTAACTCAAACCGTTTTCAATTTATTTAGACAACACAAATTAAATGTTAAATTCATGAACAACGCACACAAAGAAGCTACAAAAGCACAAGAAGTTAAAAAACCTGCAGCTAAACCAGCTACAAAACCTGCTTCAAAATCAACTGCTCCTAAAAAAGTAGTTGCTGACAAAAAAGTTGCAACAAAAGCAGCAACAACTAAAACTACAAAAGCTGCTGCTAAACCTTCAGCTAAAACTGTTCGTAAAACTACAAAAAAATAGTTATATCTAGATTATGAAAATAAATTTTTTAACTCTTTTTTCAAATTACTACAAACCATTTGTAGAAGAATCAATTATTTCAAAAGCTATTGAAAAGAAATTGATTGAAATTAATGTAGTTGATTTTAGACTTTTCAGCAAAGATAAACATCACAAAGTTGATGATGAAATTTTTGGTGGAGGTCATGGAATGCTCCTGCAAATAGAACCTATTGATTTAGCACTTGATTCTCTTAAAGAAAGAGGAGGTTACAAAATTCTTGTAACTCCTCAAGGAAAGAAATTTGATCAACAAATTGCTAATGAATTAGCTAAGAAAAAAGAAATAACTTTTATTTCTGGTCGCTATGAAGGCTTTGATGAAAGAATAGTAGACCTTGTTGATGAAGAAATTTCAATTGGTGATTATGTTTTAACCGGAGGCGAATTACCTTCAATGGTAATGGCAGACAGTATCATAAGATTAGTTCCTGGCGTAATAAGAGAAGAAAGTTATGCTAATGATTCATTTCAAAATGAAGGTTTGTTAGATTATCCTCAATACACTAGACCAAGAGTGTACAAGAGCAAAGCTAAAAAAGAAGCTTTAATAGTGCCTGAAGTTTTAGTTAATGGAAATCATGCAGAAATTGAAAAATGGAAAAAAGAAGCTCAATTAAAAAAGACAAAAAAAAATCGTCCAGATATAATCGAAAGGATTAAAAATGAGAAATAAATTAATTGAATTAGTAGAATCAAACCAATTACGTACAGACTTCCCAGAATTCAGAGTTGGAGATAATGTTAAAGTTCACGTTCGTATTCGTGAAGGTGAAAAAGAACGTATCCAAATTTTTGAAGGTTTAGTTATTAGCAAAAAAGAATCAGGTACACGTGAAACATTTACAGTTAGAAAAATTTCTTTTGGTATAGGTGTTAATAGAACATTTCCATTACACTCACCTATGATCGCTTCAATTGAAGTTGTTCGTTCAAATAAAGTACGTCGTGCAAAACTTTACTACATGAAAAACAGAAGTGGTAAAAGTGCTCGTCTTAAGGAAATTAAACGTACAAAATAATTTAACTTTTATTAATAATCAAGTAGCTCAGAAGGCTACTTTTTTTGCTGTTTTATAGGCTTTTTAGTAAAAAAAGCAAAATAAAATTTGTGCCTTTAACACAAATAATATTGATGTTTATAACAATTCATTATTTATTTTTATATTTACTTGATAAAAAACTCAGCCATCAATTCTAACATGAATTAGGTCGCCATCTAAATTATGACTTAATGAAGCATTTCTTTTCTTAAAAACATTTAATTTATCTACAGTTTTACCATCAACTAAAAGTTCAATATATGAAACTCCAACCAATACTTCAATAGTTTTATCTTTATAGTTAACAACTTTATAATTAGTTCCAATAGTAAAATTAATAAATCAAATTGTTATTATGAAAAAAACAGCTGATATGATAATGGCTATTATTGAACCAATTGAATTTTTAGTTGTAACAACTATATGAATTATTGGAAATAATGCTCCAGCTAATCCAATACTTATAAAAAGAAATATTAAACTAGCAATTATCATTCCTGTACGTTTCTTTTTAAATTTTTTTCCTTTTTCAGACATATGTCCTTTCTTTATTAAATAATGTTTTTTGTAATTATTTATGATACTTTTTATTATTAATGATCATAAATGAACGATAGATTTGTTCAGCTAACATAACTCTAAACAATTGATGTGGCCAAGTTAATTTTGAAGAATTAATTTTCATATTGCATTTAATTAATTCTTCATTAACTCCATCACTGCCGCCAATTATAAAGGTTAAATTAACATTGTTATTAACTATTTCGCTTAATTCAACTGAATCTAAACTTTTTCCTTTTAATGAAGCATAAATAACAAAACTATTATTATCAACATAAGAATTAATTAATTTTGTTTCCTTGTCTTTCTTGACTTCGATATTTTTTTCCTCACTCAATTCTTTTACTTCAACAACATTTAATTCAGCAAAATGACTTATGTTTTTAGCATATTGATTAAATAAAACTTTAAATTCTGGACTAAGTGAACCAACTGCAATTATTTTAATCTTATGCATTATTCTTTTTTCTTGTAGTTTCTAAATAAAACATAAGCATTTGAATATCAGCTGGATTAATGCCACTGATTCTACTTGCTTGGCCAATTGTTGTTGGTTTAACTTTGATTAATTTTTGTTTAGCTTCTGTAGCAATATTATTAATTTTGTTGTAGTCCAAGTCTTCTGGTATTTTGTAATTTTCTAGTCTTACAGCTTTATTAGCATCACTTTTTTGTTTTTCAATATAACCATGAAGTCTAACCATTGTTGTTAATTCTTCTAGGTATGGAAAATCATGAATGATATCATAAGGATCAACTGCAGGACGAGCTAAAGTTTGTAATAATGAGATACCTGTTGTGATTCCATATTTTATAGCCACAGGATCTTTTGAAGAAAGATAAGTAGTATGAAGTCTTTGAATTTCTTGGTCAATTAATTCATATTTTTTAATAACCTTTTGATATTGATGTTTGTCAATTAAGCCTATTTTATAGCCATATTCGGCTAAACGAATATCAGCATTATCATTTCTTAAAAGTAATCTATATTCAGCTCTTGAAGTTAGCATTCTATAAGGTTCTTTAGTTCCTTTAGTAACCAAATCATCAATTAAAACTCCTATATAAGCATGGTTTCTTAAAATAACTATACCATCTTTTTTTTCTAAGTTTAAGGCTGCATTAATACCTGCAATTAAACCTTGAGCAGCAGCCTCTTCGTAACCACTAGTTCCATTAATTTGACCTGCAGTATAAAAATTTTTAATGATTTTAGTTTCTAAAGTAGGTTTTAATTGCAAAGGATCAATTGCATCATATTCAATAGCATAAGCATACTTTTGAATCTTACATTTTTGAAGACCAGGAATACTTCTAATCATTAGTTCTTGGACTTCAACTGGCATTGAAGTAGAGAGCCCATTAACATAAGTAATTGAACCATCAGCAGTTTCAGGTTCGAAGAAAATTTGATGTCTTTCTTTTGAAGGAAACTTAACAATTTTGTCTTCAACTGAAGGACAATAACGAGGGCCAATACCTTTGATTATTCCTCCATACATAGCACTTTTAGTTAAATTTTCTTCTATTATTTTGTGAGTTTCAGGAGTTGTATAAGTTAAGTAACAAGCAATTTGACGAGGTAATTTTACATTTGATCTAGATGAAAATGATAAGTTAGTATCACTTAATACTTCTTGTTCTACTTGCGAGTAGTCAATTGAATCTGAAAAGATTCTTGGAGGTGTTCCGGTTTTAAGTCTTTGAATTATAAAACCATGTTCTTTTAATGAAGCACTTAATTTAGGTGTAGTTTTTTCATTATCAGGTCCACTGATTTTAATAACATCTCCTCTAAGAATTCTACTGTCCATATATGTTCCAGTAGTTACAACACAGACCTCAGCTTCAACTATATCGCCATTAGCTAGTTTAATTCCTTTAAATTCTTTTTTACTATTTACTAATATTTCTTCAGCTATATCTTCAATAAGATCTAAGTTAGGTTCTTTTAACACGTATTCTAAAGCTATCTTCATGTATTTTTCTTTGTCAATTTGAGCTCTAAGTGCTCTAACTGCAGGACCTTTTGATTGATTAAGCATTTTCAATTGGATCATGCTTAAATCAGCCCAAAGTCCTTGCATTCCGCCTAAGGCATCTATTTCTCTAGTTATGATTCCTTTGGCTGGTCCACCAATTGAAGGATTACATGGCATCATACCTAAACGTTCCTTATTTAAAGATATCAAAACAGTTTTATGATTTTTGTGTGCTAAGGCAAAAGCAGCTTCTAAACCAGCGTGCCCTCCACCTATAACAACAGCATCATATTTATGTACATTATTACTTTTCTTATTCATAGTTCTTATATTTTAGTTTATTTTTTAAAAAAATATATTATAATAATAAAGCAATTTTAATGGCGGATGTGGTGAAGTGGTCTAACACAGCGGGTTGTGGTCCCGTCATTCGAGGGTTCGAATCCCTTCATCCGCCCCATTTTTTTTATTCCTTTTTTCATATTTTTTTCATAAATTTTAAATAATTAAAATGCTGCTTATTTTACTAATTTAGCTATAAAACAGAGTTATTAGTCAAAACACGGTCAAAAATATTTTATTAATTAAATTTAAAAATTTAAATAATTCAATATAATTAAATATCAAACTTAAGGAGAAAAATATGAAAATAGGAAATTATAAAGTTGCATTACAAGCAATCGAAAAATATAAGAATATTATTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATAAAAAAGAATTATCCAAAGAAAAAAGTTTTTACAGTTGGAGATAATGTTCATACTTTTGATTTTATGAGTTACCACTATGACCCAATTGAAACGGTTGATTTTAAAAATTCATTAGGTATTGTAGTTGATGCTTCAAGTAGTGATCGGATTCAAGATGGACATTTATTAGTAGAAAAAAAATGTACAGCTGCTTTAAGAATTGATCACCATCCAAATGGTTCAGATATCGAATATGATTATTTATGGATCGATGAACACTATGTAGCTGCGGCTGAAATGGTGGCTATGTTAGCTAAAAGTGCAAAATGAGAAATCACTAAAAAAGCTGCCGAACATATTTATTTAGGAATAAACACAGACTCAGGTAGATTCTTATATCCAGATACTTCTGCTAGAACATATAAATTAGTAGCTCACTTAATTGAAAAAGGTGGTTTAGATTCTCAATGAATCTTAAGAGAATTAAACAAAAGAAGTTCAAGAGATATTAAATATACAGGCGAAATTCTTTCAGGATTCAAACAATCGGGCAGAGTTCTATATTTTGAAGTAACAAAAGAAATTATGGATAAATATGGCATGAATGATTTAGAAGCCGCAATATTTGTTAATGAATTAGCAAACATTGATGACAATTCTTGTTGAGCATTCTTTATTCAGCTTGCTGATGGCACAGTTAGATGTAGACTTCGTTCAAATGGTCCATTAGTTAATAATGTTGCTAGATTGCACAATGGTGGCGGTCATGATAATGCTGCAGGTTGCTCAATTAATTCTTGAGATGAAGTACCAGTAGTATTAAAAGAATTAAATGACTGCATTAAAGAATGAGAGGCAAAATAATGGGACACATGAAAATTGGTAGCTTAAAAGAAGCAACAAAATTAATTAAAAAATATGACTCAATAGTTATTTTTCATCACATTCGTCCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATTAGAGCTAATTTTCCAGAAAAGAAAGTTTACACAGTTGGCGATAATGAAGGTATGTTTCCATTTTTAAATCTTAAATATGATAAATGTCCTTCAGATAATGTACTTAAAAAATCTTTAGGCATTATTCCGGATGCAAATGGTAAAGAAAGAATTCAATGTCGAGAAATTTTAGATAAAAACTTGTTTGCTGAAACACTTAGAATTGATCATCATCCAAATGGTGATGACTTAGATAAATGTACCAGATGAGTTGAATCAGATAGAATTGCAGCAGCTGAAATGGTAGCTGACTTAGCTTATACAAACAAATGAAAAGTAACAAAAAGAGCTGCTAATTGCATTTTCACAGGTATTGTTACTGATTCAGGAAGCTTTAGATATGGTGATACAAATGCCCGCACTCACCTTTTAGCTTCATATTTATATGAACAAGGTTTAGAGGCTGACAAAATATTCCTAGGTCTAGCAGCTACACCTTTAGACGATTTAAAAATTAATCAAACATTGTTAAATAATTTAAAAACAGTTGGTAAAGTTGCTTATACAACTATTGATTTAAAAACAACAAAAGCTTTAGGCAAAAAACCAAATCAAGTTGTTAGAGTAAATACAATTGGGAATATTATTGGTTATCCATTTTGGGTTCAATTCAATGAAGAAGAAAATGGCAATGTTAGAATTGAATTTAGATCAAGTGGTCCTTGTGTTAGAAATGTTGCAGTTAAATGAGGCGGCGGCGGCCACGAAAGAGCCTCAGGAGCTGTTATTAAAAGCTGAAAAGATTTAGATGCAGTTATTAAAGACTGTAATGCGGAAGTTGAACGTTGAGAAAGAGAAGGCAACGACAACCAAGAAAAATAATTTTAAGACTTGCACGAGTGCAAGTTTTTTTGTTGACTTAAAAATCTTGTGATATAATAAAACCATCAAGTGAATATTACAGAGTTGCAACTTGCAACTCTTTGTTATTTTTGTTGGAGGTTTCATGAATTGAAAAGAAATACTTGAAAAACAATTTGGAAGTCAAATTTTAGAAGCAAAAATTATTGAAGAAGATGACTTAAAATTGTTAGACATTAGTGTCAATTATCATGACTTAAATAAGGTTGAAGAAATATCAAAACAACTTAATGAATTTATAGATTCTAATTATCCATCTTTGGATATTGATTCATTATCAATACATTCTCCAGGTGTCGAATTAACCTATAAAACAGATGAATTAGGAAAACACATTGGAGAAATGTTAACTATAAAATTAAATAAAAGTATTGAAAAACAAGACAAATTTAATGGTAAATTATTAGAAGTTTTAGAAGGCAAAATTCAAATAAGTTGAAACAACAAAGGCCAATTTAGAAAAGTATTTATCGACAAAAATAACATAGTAAAAGTAGAAAAATATATAAAGTTTAATTAAGGAGAAAAAATGCCAAGAAAAAGTAAAACAGAAGCACAAGAAACTTTTAATGCAAGTAAAGCTTGATATCAAATTTTGATAGGTTATTTTGAAAAAGAAAAACTTCCTATTGAAGAAGTTATCAAAATTTTTTCAGAAGAAACTACAAGAATTATTAACAAAATCTTAGATCCAAATGCTGAAATAGTTTTTGAATTAGACAAAGAAAAACAAGAAGTTCACATTTTCAACAATAAAACATGTGTAACTAAAGATGAAGAATTATTGCTTGATCTTGAAAATCAAATAAGTTTTGTTGGATATAAAGAAGCAAAAGTAAGAATGTCTAAAGTTAAAGAAGATGATGAAATTAAATGAGAAATTGACTTAGGAGTATTAGACAAAAGTAAAAATCCTGAAGCTAAAAAATCATTAAAAATTATCAACTCATCAATTGCACAAGCTATTAAAACTCTTCAAAAGAAAATGGTTTTTGAACAATACTCAAGCAAAATTGGACAAACTGTTAAAGTTGTATTGAATTCAAAAAATTCAAATGGTTCATGAAATGTTCAAATAGTGAACAGTAATGTTAATGCATATTTACCAGCAGGATTAATTAGTACAAAAAGAAAAGCAAACCCAGGTCAATATTTTGATGTTGTTATTGAAAAAGTTTCAGAAGAAACAAAATTGAGTCAAATTGAAGTTTCATTAGACTCACCTAAAATTGTTTGAAATATTTTACAAAACAATATTCCAGAAATTGCTGAGGGTTTAATTGAAGTTGTTGATGTAGTTCGTCAACCAGGAGAAAGAGTTAAAGTTGCAGTTAAATTAGCAGAAGGCGCAAATCCTGATATTGATGTTAGAGGTTCAATTATTGGTGAAAATGGATCAAGAATTGATTTATTAAGTCAAAAATTAGATGGTGAAAAAATTGATGTTATTCTATATGATGAAGATATCAAAAAATATGTTGCTAACTCAATTAGTCCAATTCCAGCTGTTGATGTTATTTTAAAAGATGAAAGAGAAAACAAATATTTAGTTATTGTTGTTCCTGAACACTTAACACCTGCTATTGGTAAAAGAGGGGTTAATGCTTATTTAGCTTCAAGTTTAACAAAAGTTAAATTAGATATTATTTCAATAACTGATGCTAAAGCTCAAGGAATAATTTTTGATGAAACATTAGTTCCTACTATTAGTGCAACCATTAAAAAAGAAGGACCTAAAATTAGATATCAAAAATCTAACAATAATAGAAGATTTAATAAGAAACCTGCTAACAACATTTTTGCTCAATCATTTGATTCAATCGATGTTTCAAACTTTGATCAAGATATTGCTGCTTTCGAATTACATGAACAAGACAGATTGGATTCATTACAAGAAGATGACTCAAACTTCGAAGAATTAGTTAAGAAAATTGAAAGCGAATATGCAGTTGAAAATGAAGTAGAAAACATTAAAAATGTTGAAGTAACAAAACCAGTTGCTCAAGATAACAAAATTTCCGCATCTGACTATAAAAAAGCTAAAGAAGTCGCAAAAGACTTTGTTGTTGATGATGATTTAAAGAATTTTGGAATTGACGATTTTGATTTAAGTGACATTGATTTTGATGAAGAAGAAAACAAATAATTTAACAAAAATTAATTCCAATTTTACTCGGAAGTGCATCATCACAGGTCTTATAAAACCAGTTGAAGAATTGGTTAGATTTGATTTTGATAAAAAAAACAATATAATTACACTAGATATTAATAGAGTCAAAAAGGGACGAGGTTGTTACTTTATACCTACAAGAGAGAATTGAGAAAACTTAAAAAAGCATAAAGGACTTAATCGTGCTTTTAGAACTGCTGTTGATCGTGAAGTATATGACAAAATAGAAAAAGAATTGGAGGACGCAAAATGCCTAGAAAGAACAGAATAAGTAACGTTGATGAAGTCAAGAACCAACTTTCAGATATTAAAACTGAAGTTAAGAATGGTGTTTTCATTTTTACAAACAAAATGCCATTAGGAGAATTTGCACAAAAAATTGGTATGAATGCCAATGATTTGATCAAGCGATTTTTGATGAAAGGAAAGCTTTATCAAATTAACTACGTACTTGATGAAGAAGAAATTGCTGAAGTATGTATGGAAAGAGGACTTGACTTTAGAAAAGAACAAAACATTGATGCTGCAAATTTTTTGCATGCAGTTAAATTTGAAGATGATGAAAAATCATTAGTAAAAAGACCTCCAATTATTACAGTTATGGGTCATGTTGATCACGGAAAAACTACATTAATTGACTTTATTAGAAAAACTAAAGTTGCTGAAACTGAAAGTAGTGGTATTACTCAACATACTGGTGCTTACCAAGTAGTTTACAAAGGCAACAAAATTACATTTATTGACACTCCTGGACATGAAGCATTTACCGAAATGCGTTCACGTGGAGCTAAAATTACAGACATTATTATTTTAGTAGTAGCTGCTGATGATGGTGTTATGCCACAAACTAGAGAAGCTATTGACCACGCAAAAGCAGCTGATGTTCCTATCGTTGTTTTTGTAAATAAAATAGATAAACCAAATAAGGATCTTGAAAGAATTAAAAGAGAATTAGCTGAATGCAACGTTATTGTTGAAGAATATGGTGGTGACGTTCAAATGGTAACTGGTTCTGCTTTAAAAGGTCAAGGTATTGAAGATTTATTTGAAGCAATTAGTTTACTTGCTCAATTATTGGATTTAAAAGCCAACCGTTCAAGATATCCAGTTGGTACTGTTATTGAAAGTAAAATAGATAAAGGTGTTGGTGTTGTTTCAACTTTAATTGTTGAAAACGGTAGCTTATATAAAGGAGACTTTATTGTAGCTGGCTCAAGTTATGGGCGTGTGCGTTCATTAAAAGATGCTACTGGAAAACAAATGGAACATGCTCACCCTGGAACACCAGTTGTTGTTACTGGATTAAATAATTCGCCACTTGCAGGTGATAGATTTATTGGTTTTCAAGATGAAAAATTTGCTAAAAAATTAGCTGAAGAAAAAGCTCAAATCGATAAGAACAGAGCTTTATATGACAAGAGCCAAACTTCAGAAATTGAAATTGGCAAAAAGGTATTTAATGTAATCATTAGATCAGATGTTCAAGGAACAGCTGAAGCTATCAAAGGCAAAATAGATGGCATGTGTAATGATGAAGCAATGATTAAAGTTATTGGCGCTCAAGCGGGACAAATAACAAATGCTGACTTATTATTAGCTCAAGCCTCAGATGCAAGGATTATAACTTTCAATGTTAAGCCATCTGCAGCTATTAAGCAAAGTGCAAAACATTCGGGAATTACAATTGTTAATTATTCTGTTATTTACAAAATTATTGAAGATATGGAAGCTCACCTTAAGGGTCAAAAAGCAGTTGTTTATGAAGAAAAGAAAATCGGATCAGCTCATATTATCAAAGTTTTCTTCTATTCAAAAGTTGGAGCAATTGCTGGATGTATGATGGATGAAGGCGTTGTAAAAGCTAATTGTAAAGTTAAAGTTTTTAGAGGCAGAAAAATGATTCATGATGGTGTAATTGAAACGCTTAAACGTGAATTAAATGATTCAAAAGAAGTTGAAAAAGGTAAAGATTTTGGTACTCACATTAAGAAGTTTGATGATATCAAGGAAGATGATATTTTAGAATTCTTTGAAGATGTTCCAGTCTCTTTATAAGACTATAAAAAAGGCAAATAGGTAAAATTATGGATTTAAAAAAATATATTCGAGATATTGAAAATTTTCCTAAAACAGGCATAATGTTTAAAGATATTTCACCATTATTAGCAAATGGTAAAGCATTAAATTATACAATCATAGAAATGTCAAAATTAGCTCAAGATGTTGATGTTATTGTTGGACCAGACGCGAGAGGATTTTTATTTGGCACGCCTACTGCAGCATTTTTGAAGAAACCATTTATTATGGTTAGAAAACCAGGAAAATTACCTGGTGATGTTATTTCAAAAAGTTACGATTTGGAATATGGAAATAATGTTTTACAAATTCAAAAAGGGTTTATTAAAGAAGGTCAAACAGTTGCAATTATTGATGATGTTTTAGCAACAGGTGGAACAACTAAAGCTATTATAAAACTATTAGAAGAACAAGGTGCGATTGTTAAAAAAGTTATTTTACTTCTTGAATTAGTAGATCTTAATGGTAGACAATTAATCACTAAAGATAATGATATTGAAATAGTTTCCTTAGTTAAATTTTAAAAATAATTTATGTTACATAAATTTAAAATGTATGCAAAAAAGACCACAATGGTCTTTTTTTAATTTTTATTATTTTTCAATTTCATCAATGCAAAATTCAAGTGTGTTATTAAGAATTCGAGAAGTAAAAGTTTTAACTTTGTAAGGCTCTCTTTTAAGTTGATCATGGTAGTAAGATTCAAATTCTTTTTTGTTCATGCCACTGAAGAAAAATGTAATTTTATTATTTGCGATTCTGCTTTCGATTATCTCCATTAATACTTGGAGTCTGAATCAGTAGTTATTTTTTTCTAAACCAATTTCGTCAATAATTAATGCATTAACTTTTTTGAGTTTGTTAATTATGTCAATAACTGCATTAGCATTTGTAGCATTGCTACCCATGTTTGAAATTAAGAATGAATATAAAGCACTGGTTGTTAAATAAACTGATGAAATACCTTCAGCTGCAAATTCGTTGGCTATTGCACTAGCAATGTAAGTTTTGCCAGTGTTGTTAGCTCCATAGACATAAATGCCTTTTAGTCCTTTTTGTTTCTTTTTAGCTATTGCAACTTTTAGATGATTAATATATTCAAACATTTCTTTTCTTTGAGCAGAAGAGAGGCGAATACGATCAAGTCTAGCTTCTGGATTAGGTTCGCAAATTTGAGTTAATCATAAATTGATGTGTCGTGTAACAACTGATTTGTATTCATTTTTTGCAGCAATCTTTTTAAAAACTAATTTTTCATTTTTTCTTGAAATAGAAAAAATCCATGGATAAATTTGAGAATTATCCAATGATTCTTTAATATTAATAAATGTAAATAAGTTTTCCAAAATTTCTTCATTTGTAATATTGTTAGCTTGAATTAATGCTTCTAGTTGCGGATGGCCATGCATTATTTCAAGTGCTTTGTTTTCAAATTCAGCCTTGGAATTAAAAAATAACTCATTTTTAATTTCTTCTTTTGCTGCCATAATAATTACTCCATTAAATATTGTTGTTGTCTTCTCTTTGAAGTGATTCTAAATAGGTAGCTTTATATAAATTAATTTTTGAAACTGCAATTTTGTTTTTAACTTTAAGTATGTCATCTAAATAATTTTCAATTGCTTCAAATGAGAAGATTTCTTTTTTAATTAAATCCTTAATAATTTTACCCACATAATTGGTATTTATGCAATTATTTATTTCATTTGAATAAAAGAATACTAAGTTTAAGCAAGGATCACCTAAGCCAGCTGCTCTACTTTCTTTAATTAAATCTAAAACATTTTGACGTGGTATTTTGTGACTAATTTGAGAATAGAAGTATCTTGAATCAGTTTTAAGTATTGCTTCATAAGGATTAGGATAATCAAAAGTATTAAGTGACAATTTGGTTTGAATTTCTTCAACTATTTCTTGAGTTTTAATCATACTTGAGTCAACTTCATGTGAAAAAGTAATAGTTAATTCTTCACTTGGTTCAGGAAAAACTTCTTCAAAAGAAGCTGAAGCATCCAAAAGATTTGAAGCTTTACTTAAATAAATATTTCTTTCACGTCCAACTAATCTTTCAAAGTTTTTGCTACCAATAATACTAATGAGTTTGTTTGCTAGAATTAAATTCTTTTTGAAACCTGTATTATCAAGTGGTTTTTCAATAACAAAAAGTGTTTTGCGATTAAACTCATCTATAAAAGTAGAAATTAAAGATACAGATTCTAATTTTATACGTGCTTGATTTAGTTTATTTACATCCATATTTAATAGATAAGTAAGGCTATCATAGTCATAAAAACCAGTTTCTGGAGATTGAGAAAATGATAGATCTCTAAGGTATTCATAAAGCAAAATTGCATCACTACCTAAAATAGGAGCGTAAAACTTGCGTAAGTTTTTTAAATCTTCTCCACTAACTGATGAAGAATGTTTGATTCTGAAGAATGCGTAAGGAATATTTTTTAGCATTTTTTACCTCCTATTTTTTTGGTCATTTTTGACCTTGTTTGCTCTTATTTTATGAGATAAAAAATAGAAGTTTTAAAAAATCTAAAATATTTTCATTATTAGTGAAGTTATGCCCATAGAAATCCACAATTGGTATAGACCCAAAAAGTTGATTTTTTATCGAAAAAGGGTCGTTTTTTTCATCTATTGCCTTAATTTTAGTTTTATCCACATTACGTTTTTTAAAATTTTTTAACCTATTTTTTTGGCTAGTTTCTAAGCACAAAACTGCAGAAAACAAAGAAATAAATTTAAAATTTTTTGGCAATAAATTTGGTATCTCAACAATGAAAAAATTTCCATCTTTTATTTCTTCAAAAATTAAAGGATAAATGATTTTTTCCAATTCTCCCAAATTATGTGCTTTTTGGTCAATTCAAAGTTTAATTTTTTCCTTACTTATACCATTTTTATCATTTAAAAAATCACCTAATTCTTGATTAATTTTGTGGTAAATTTTGCCATTTTTTTGATATTCACGATTAATAAACTCGTCACAATTAAAAGTTTTAAGACCTAATTTCTTAAAATTTTCTAAAAAAGTACTCTTTCCCACGCCAATTTGACCAATTAAAGCAATCATTTTAGGCTCCCTCCTTTATATATTCTTCTATAGCTAAGTTTGCACTTTTAATATAAGCTCTAGCTAAACCGTTGGCTCCCAACTTAATTCCACCAAAATAGCGAATTACAAAAACAACAACATTATTAATTTTGCGCATAATTAAAAGATCTCTAATTGGTCTGCCGGCTGTACCATTAGGTTCGCCATCATCGCTAAAGCCTGCATTGATCACTCCATTATTTAAAAAGCTATAACCATGACAAATATGAGTTGCTTTCTTGTATTCTTTTCTTAAATTAGCTTCTACTTCTTTTATTTGTTCCTTACTAGTCACATTAAAAGCTAAAGAATAAAACTTTGATTTTTTGATTTCATATAAAAGTGCTTTTTCCATATCTATTATTATATAGAAAGCTGAATAATAAAAAATAAAGTGGACTTACTAAGTCCACAAAAATTATTAGAATTCATCGATTTGATCGATTCTTGCTTGGTGTCTGCCACCTTCATATTCAGTTTTAATGTATTCGTTAAACATAGCTTGTGCTTCAGCAAAAGGTACATATCTACCACCCATAACTAAAACATTAGCATTATTATGTAATTTAGCAAGCCTTGCATCTTCAACTGTAACTACTCTAGCTGCGCGAATGTGTTTATGACGGTTTAAGGCATATGAAATACCTAAACCTGTACCACAAAAACCTAAACCAATTGTGTTTTTGTGTTCATCAATATATTTAGCTAAGTTATGCCCTTGTTCAGCATATGAAATACTTTTTGAGCCATCCGCAGGTCCTAAGTCAACAACTTCATAACCTAAACTTTTTACATAATCGCGCATTTCATTTTTTAATTCGCAACCACCATGGTCGCTAGCAAGAGCAATAATATTTTTTTTAGCCATATTAGCCTCCTTAGTGTCTATTTTTAAATGATAAAGCAAAATTCTGGAAAAGTTTGGTAAAAATTTATCTTTTTTCCTAAATTTTTCCATTTTTTGTGTTTGTTAAAGAAAAAAAAGAAAGTTTAAAGTGCTATGAGAATAAAAAAACAAGAAGATATAAAAGACTGTGGTTTATATGTTTTGCAGTATTTTGTGGCAAAACATAATGACGACTACATTGATATTAATTATTTTAAAAATAAGGCAACTTACTCTTCAGAAGGAGTAAATATTTCAACACTTAAAAAATTAGCTTCTGAACACGATATCGATTTAAATAGTTATACAGGTGATTTTGAATCTCTTGAATCTTTGGACAATTCAAATATGCCTATAGCTTTATTATTAAATAGAAAAGGATATAGCCATTATGTAATTTTAGAAAAAATGAAGGACAATTATTTTATTGTTCAAGATCCAGCTTATGGTCGCAAGATGAAAGTTAAAAAGAAAGACTTGGCTAAGGAATTTGCCAATGTGATACTATTTTTTGATCCAAAAGAAGAAAAATCAAAAAATAAAAATAAAAACTATTCACTTGATAATAAAATAAGCACATTATTTTCATTTAAAAAATATACTTATCCTTTATTCATTTCAGCACTTTTAGGATTGTTTTTATCATTTGGATCAACATTTTTTATAAAAATAGTTTTTGATTTTATATTGCCTAATTATCTTAAAAATAATTTGATTATTATTTTTATATTTTTCCTATGAATTAATATTCTGATGTTTATTAATCGGTTGTTTAAAAATCATGTTATCAAAAAAATAAAAAACGATTTAGAGTTTACTTTAACAAAAGCATTTTTTGACAAAATAAAAAGAGTGCCATCAAATTATTTATCTAAATTAACAAGCAATGATTACTTTAAAAGACTTACTTATATAAAAGAAATATGTGAATTTCAATCTAATTTTGTCTTTACTTTTTTAAGTGAAATATTTTCTTTTATAGGTTCAACAATCTTATTGATTTGAATTAATTATGTTCTATTTTTAATTATGTTTGGTATTGCTATTGTTGTTTTATTAGGTAATGTTATTTATCATTTTTGAATAGAAAAAAACTATCCAGATCTCTTGGAGAAAAATTTAAACAGCATGAAATCAGATATTGATTTTATATATTCCTGAAAAGAAATGGATAATGTAGATTATTCAAGATTTATTTCTAAAAACCAATTTAAAAACATGTTGGAATTTAAGAAAAAAGAATATTCATTTGCTTCTAAAGATGATATTAAAAACTTTTGAAATGATCTTTTTATTGGCAACTTTTCAACCATTATTGTTTTTGCTTCTAGCTTCTTTATTTTAAAAAATAAGTTATCAACTGGTTCACTAATGATGTTCTTAAATGGAATAAATTTCTTTGTTAATCCAATGCTAAATATGAGCTCATTATTACTTTCTCAAACAATGATCAAAAGAAATGTTAATTTAGTAAATTTTGTTTTAAATTTAGACGAAAAAAGCAATTTAGATACTGGTTTATTCATCGATAAAATTAAAAAAATTGAATTAGAAAATGTTAATTTTTATTACGAAAACGGAAAAAATATTTTAAATATTCCTTCATTTACGATTGACCAAAATATTCAATTAAATGGTAAAAATGGTAGTGGTAAAACCACTATGCTAAGTTTTATAAACGGCTGTTTTAAAGGTTATTTAGGAAAAATGAAGATTAATGATATTGATTTTGATTCAATAAATTTGATTAATTATCAAAGTAAAAATATATTGATAAATTCTTCCAGCCATTTACCAGATGTTTCTTTTCTCGAATATATTACAAGTAACAATGAAAATTATAAAAATGAATTGAATAATAATATTCAAAAATATGATCTATACAAAATATTTGAAGAAATGAATTTTAATTTAAATAGCAGCATAATTAGTAATGGCAGCAATTTGTCAAGTGGACAAAAACAACTTTTATTATTACTAAAATTATTTGTAAGAAAATATGACTTAATCATGCTTGATGAAGCCTTTGAAAATATTAATCCAAGTAATTGTGAATTATTGAAAAAGGCTATAAAGAAATATCAAGAGGAAGCATTATTTATTGAAATAAGTCACAATAGGAACTATTTATTTAAAACAAAGGAGGTGTGCTTTGAAAAAATTAACAACTGTTTCTAAAACTGTGTGAATATTGTTAATATTGTTAGCTATTGCAACAGTAACTTTAATTGTTTTTATTTGTTTATATCATGTTGACAAAACAATAAGAGTTAAGTTACAAGTTAATGACAAAAAAGACTTAACTTTGTTAGCAAATGATAAGACTGCTTATTTATTAAAGAAAAATCAAAAAATAATAATTAATGTAAATGACCAAATTTTCAATTTAAAAATCCGCAACTTAAAAATTGAAAATCAAACGATTAAAGTAACCTTCTATAATTTGCCTAATAGTTTGAAACTTTTACCTAATACAACTTTAGATGCCACGATATTTGTGGATCGAGCCACTCTATTAAATTTAATATTAAATTCATAATTAATAATATAATTAGAATTATGGTTGAATTAAATGTTGTAAACAAGACTAAAAATAAATTTTTGTTTGAAAAAGAATACAAGCAAATATTAGAAAATTTAGCTAAAGAATTTGGGCTTAATCAAACTGTTTCAGTTGATGTAACTATTACAAATAATGATGAAATCCAAAAACTGAATAAAGAATATAGAAACAAAGATTATCCAACAGATATCTTGTCTTTTGGTTTAGAAGATTGAGAATTATTTGAAAATATGCCAATTTATCCTTTAGGTGAATTAGTAATAAGCCATGAAAAAGTTGAAGCACAAGCTCAAGAGTTTGGTCATTCAATTCGTAGAGAATATTGTTATTTATTTGCTCATGGATTAATTCACTTGATGGGATATGATCATGAAACAGAAGTCGAAAGAGCTGAAATGAATAAACTTGTTGATTTAATATTTGAACCATTAAAAATAAGTAGAGAGGAATAATCTATGGACGTTAAAAAATTAAAATCTTTATTGAAGTATTCATATTGTCCTTGATCTAAATTTAAGGTTGCTGCTATTGCAATTGATGCTGAGGGTAGAGAATGACCTGGGGTAAATGTTGAAAATGCTGCTTTTCCATCTGGCTTATGTGCAGAAAGATGTGCTCTTTTCGGATCAGTTGCTCATGGTGCAAAAGTTGGAACATTTAAAGAAATTCACATTATTTCTTCTGGAGATGATTTTATTTCTCCATGTGCAGGATGTCGTCAAGTAATGACAGAATTTATGCCCGATGATGGTTTAATTTACCAATACAATAAAAATGGCGACAAATGTAGAGTAAATAAAGTAATTGAAATGGTCCCATTTCCTATTAGAACAGAGCAAATTAAATAATGAAAGTATGTTTAGCTAGTATTATCGGCAGACCTAATGTAGGAAAATCATCATTAATAAATCAAATTGTTAAATATGATGTTGCAATTGTAACTAATGTTCCGCAAACAACAAGAGATCAAATTGTCGGCGTTTATAACGAAGATGATTTTCAATTAGTTTTTGTTGATACACCAGGTATCCACAAACCATTAAATTTATTAGGTGAAGCCTTAAATAAAAGTGCTTTTGAATCTACAAAAGATGTTGATTGTTTAATATTTCTTTCTCCTGTTAATGAAAAAGTTGGACAAGGAGATATTTTAATTTTAGATAAGATCAAAAATACCAAGAATAAAATTGCAGTCATTTCTAAAATTGATTTAGCCAAAAAACCAGAAGAAATACAAGAAAAAATTGAAGAATTAAGCAAATACAGTTTTGACAAAATATTGTCTGTTTCAAGAAATAATGAAAACTCAATTGAAGAATTAATTAAAGTTATTAAGCAATATGCCTATGAAGGTGAGCCATTTTATGATGAAGACTATATAACAGATAAATCAATGAGATTTTTAGCAAAAGAGATAATAAGAGAAAGTGCGATTAATCTTTTAAGAGAAGAGTTGCCACACTCAATTGCTGTAGAAGTTATTGATTTTATTGAAGAAGATGATATTAAAATAAATGCCATCATTTATGTCAAAAAAGCTTCTCAAAAAGGAATGGTAATTGGCAAAAATGGTCAAATGATAAAACAAATAGGAACCAACGCAAGAAAGAAAATGATGAGTCAATTTGATTCAAAAGTGGAATTAAATTTAAAAGTTAAAATAGCAAACAAATGAATTAATGATGGTAAAGCTCTTAAAAAATTTGGATATGAATAAATTGTTATAAAATTAACAAAAAAGGAGAAGTTATGAATCGTAAATATTTAGATAAATTATTTAAAGAAAAGAAAATTGATGCTTTAGTTTCTGAAGCGCCTCAAACAAGACTATGATATTCGAAAGTACAAACATCAGATGGATATATAGTAATTGAAAAAAATAAAGCTTATTTATTCGTTGATGGTAGATATATTGAATATGCAAGAAATAACGCAAAAAATGTAGAAGTTATTTTATTACAAGCTGGTACATTAAAAGAATTTTTTGACAAAAAAGCCTATAAAACAGTTGGTTTAGAAAAAGATTACTTAAATTACCAAGTATATGAAAATCTAAAAAATCTTATAAAACCTAAAAAAATCGAATGAGTTTTAGGACAAGAATTAAGAATTCTTAAATCAGCAGAAGAAAATAAAATTATGCAAAAAACTATTGATATTTCATTAGCTGCATATAAAGAATTAATGACATGAGTACAACCTGGAATGACAGAAAAAGAAGTAGCTGCATACTTAAATTATTTAATGAAAAAACATGGATCAGATAAAGAAAGTTTTGATGAAATAGTTGCTACAGGGCCTTCATCAGCTGAACCTCACCACCATCCAACAGATAGAAAATTAAAAGAAGGGGATCTTCTTAAAATCGACTTTGGTGCTCTTTACAAAGGATACAGTGCAGATATTACTAGAACATGTATTTTAGGTGGAGACAAAAAAGCGAATGATCCTAAACAATTAGAAATTTTACAAATAGTTATGGAAGCTGCAAAAGCTGGTCGTGATGCAGTAAGACCTGGAATTAAAGCAAGTGAAATTGATAAAATTTGTCGTGATTACATAACTAAAAAAGGATATGGTAAATACTTTGTTCACTCAACTGGTCATGGGCTTGGAATTGATGTACATGAATTGCCTAATGTTAGATCAACAAGTGACTATATTTTAGAACCTGGAATGATAATTACTGTTGAACCTGGTATTTATATTGAAGGTTTAGGTGGAGCAAGAAATGAAGATGATGTTCTTGTTACAGAAAAAGGTCGTTATGTATTCAGTAGACCTGAAGAAAAATCTTAGAATTTATTTTTAATAGCTTTAACATTTTGTTAAAGTTATTTTTTTATTGTGGAAATAATATAAAATTAGAAATATGAAATATGAATTAGGCCAAATAATAAAAGGAGTGGAAGCAATAAATTTTACTTATGAAGCATTAGGCTTAGTTAAAGTAGATGATTATTCTATTTTTGTTGAGAATTTATTGCCACAAGAAAAAGCAGATATACAAATTAAAAAATCGAACACAAAATTTGCTTTTGCTATAGTTGTAAAAAGATATAACGATAGTCCAAAAAGAATAAAAGTTTTAAATCAAAAATTAATGCTAAGTGGTTCAACTCCATTAGCAATTTTAAACTATGCAGATCAATTAGAATTTAAAGAAAATGTTGTTAAATATTTATTTCAAAGAAATATCCATTTTAATGAAACACAAAAAATTTTGCCGTGTGAAAAACAATGAAATTATAGAAACAAAATTACAGTTTTTGTTGAATCATCAAAAGGAAAAATCAAATTTGGTTTATATGAAAAAAATACACATAAATTAGTTGAGCAAGATTCTTATGATTTGGCGAATGAGTCTATAAATAAGCTTCTTATGTATTTAAACAAAAATATTAATAATTACAAATGTTTTATAGAAAACGGACAATTTTTAAAACAAATTGTTGTTAGATATTCAGAATCATATGATCAAATGATGTTAGCTTTTGTTCAAGACAAACCATTTGAATTATCTGAAGATTTATTAAAAGATTTAACCTCAAATTTTAAAAATTTAAAAGTTATATTAAAAGAAGAAAAAGTTAAAAAAACAACTTATACTTATTATTCAGAAGCTAAAAACATACAAGACAAAATTAGCAATTTAACTTTTAATATTGATTACAATAGTTTCTTTCAAGTTAACTCGTATCAAACCAATAATTTATATAACTTGCTAGTTGATAATTTGCACCTTGATAAAACTATGAATGTAGTTGATGCATATTCTGGAATTGGAACAATAAGTTTAAAAATTGCAGAAAAAGTTAAAAAAGTTTATGGACTTGAAATAGTAAAAGAAGCAGTTATAAATGCTAAGCAAAATGCAAAATTAAATAACATAAATAATGTAGAATTTTATGAAGGCGATGTTTTAAAAACTATTGATAACATTAAAGAAAAAATAGACACAATAATAATAGATCCTCCAAGAGCAGGAGTATCGAAAGAAGTTTTAGAAAAAATATTAAAAATTGAACCAAAACAAATTGGTTACATAAGCTGTAATCCTCACACACTTTGCAGAGATATAGATTTATTAACATTATCAAAAAAATACAAATTAGAATTTTTAAAACCATGTGATATGTTTTGTCAAACACACCATGTGGAAATAGTTGCAATTTTAAAATTAAAATAAAAAAAGAAAAGTTGTTTTAAGTGAGAGATGTGACTTAATTTTGACACACCAATAAATATGGAATTAGAATTTTCGGAATTTAATTATTTATTTAAGCTCCCTATTCAAATAGTAAATTTATTCTATGGAAAAAATGATTAATGCTGCGAAGCTAAATTCAGAATTATTAAAACTTTTGGTATGCAATTAGATAGCGAAAGTATTAAATCTTGATTACAACCTATTAACAATTCCTTATTTGTAGATTTTCCAAAAACATTTAATAAAGAAATCATTGTTGATGAAATAATGGAAGACATTACTTATGATTTATATATTATTTATTGGCAAATCTCTATGCTTGCAGCAACAAAAAACAAGACAATTTAATCAAAATAATCAAGCTTTATATATGATATAATTTTTATTATTTTAGTAAGGAGAAACTATGGGAGTTACAATTAAACCAAAGAGTTATTTAGATTTTGTGAATGAAAACTTACAACCATTTAAAGAAATAGTTTCAAACAGTTTTGATTCAGTTATTAAAAATAAAAAGAACATTTTGTCAAAATTAAAAATATTACTAATTGTTTCAATAGTACTATTAGTTTTAGGTGGAATTGGATTAATATTATCTTTCTTTTTCTTCATAAAAACTAATTCAAAAAATACAGCAATATTTGCTGGATTAATTATTTCTGCAATAGTAACAGGAATTGGTTCAATTGTATTATCAGTATTTTTAGGAAAAAGGAAAAAACTTTTTAAAGAACTCAAACACGTTCTTGATAAAGAAAAATTATTTGACAAAGCTTTTGAAAAATTATCATACAAAGCAATCGATCCAAATGACCCAGAACAAGCACAATTATTAGAAAAATTTAAAAAAGGTTATGATAAAAATAGAGATTGTTTAGACTGAGTAGATCATATTCCTGGGACATATTGAATTCCTTCAGATGCTAATGTAGACTCAACTGGAAAAATTTATTTTGTTATGGACAAAAATGAAAATCCATGAGCTATTCAAACTGTTCGTTATCATTGAGTAAGAGAAGAAAGAGACTTAAAAGGAAATATAACAATTAGACATATTTATGATTATGCAACATTATTTGAAGGAATCTTCTACTCTCTTCCTAAATCTAAAAACTTTACTTTAGCAATTGGAAATAGATGTGGATTAACTAAAAATTTAAAACAAAACATCAAATATGAAAATCAAGAATTCGACAAATTATTCAAAGCTTATTGTTCAGATTCATTGAAAGGCCACAATATTTACCAACCTTATCCAATGGAGATTACACTTGAACATTGCAAACAAAATAAAAGTTATGTAAAATCATTTGGTATGCTTTTAGATGATCAAAACATTAAATGTTGAGTACGTCCTCGAGGTTCAATTTTAGAAATTGATATGCCTTCAAACACAATTAATAAAGATAAAATTGTTAAAACTGTATGTAAAGATATTCTTGAAGATACATATTCAGTTTACTGATTATTCTCACTTCTAACAATACCAGGATATTTCAACTAATTTTTAATAAAATGAAGTAATGTACTTCATTTTATTTTTTAATTTTTTAGTAAAATTTATTTATAAAATTGGAAAGGAATTTATGGCTAAGATTTTTGTACCAAAGACTTTTGCGGAATTTAAAGATACAAATAAACAGTTTATAAATGACAAAATACAATTAATTATAGACAATAAAAATAAAGAAATTAAGACAAGCTTTTTATATTTAATTACATCAATTGTTTTAATATTTCTAGGAATAATTTTTTGAATTTCAGGCTCACTTTGTTTATTATTAATAAGTTCTTCTAAATCGTTTTACTGAACAGGATATTTACAAATAGCATTATTCTTAGCTTTAATTTTTGCAGGAACAATTTTATTAATTACATTTTTGAGAAAACAAAATTCGATTGTTACAAATATTGACAAAGGTCTAAACGAAGAAGATATTTACAAGCAAATTTTTAACCAAATTAACTATAAAATAGTTGAGTCGGAATATTTAGAAAATGTAAGTGAACAAGAAGAAAAGAATTTTTTATCAATAGAATTAAAGAAAAAAAGTAATATAGAAGAAAGAAAAGAATTGACTAAAAAAATAAATGATATTTTACTTAATGAAGTACCTTTTAACTGATTAAACAATTTTCCGGATGGTTCTTGAGTGTCTAAATCAGCATTTATTGAAGAAAAAACAGATTCAAAAGGAATAATAGATAATCAAAAAAATCATTGAAATATAACATCAGCTAAATTTTCATGGGTAAGTAATCCAATTGACAAAATGTCAAGTATAAGAGAAAAGGATAAAAGTCAATGAATAATATTTTTTGAAACTAAACTCAAAAAGTTTAGTGAATCAAGTAACAAAAACTTTTCTTTCTCATTTGGAAAGAGAAGCGGAATAAACAAATTATTTAAAAACAACATTGGATTTGAAAATAAAAAATTTAATGATATTTTTAGTCCTTATTCAAATGATAAAATAAAATCCATAAAATTTTATGGACCATATGCAATGGAAGTTTCAGTTAATCACTTTGAACAATATTTTGATAAAAACAAAGATTTTGGATTATTTTTAAAAAGAGATTTAATTAGAGGCTGAGTAAAAAAGAATACAAAAATATTAGAATTAAATTTACCAAAATACATAATCAAAAAGCAGAATTTTATTGACAAAGTTGTAAACAATTTGTTAGATCAAATATATTTAGTTTATTGATTTATATCAATGGTTCAAATTTCCAATTATTCATAAAGGAGAAAAAATGAAAGAAACATTTAATCAATTTTTATCTCGTTCATATGTTCCTTTGTCTAAACATTTAAACGAACAATTAAATAAAGAAGATTCAAAAAACAAAAAGCTTAGTAAATCTTTCTTATGAAAAATAATAATGGGGTTGTGTCTATTAATTATATTGGCTTCAATTTCAATAGTTCTGATGTTGTCATTTTTTGAAAAGTCAGATAAGATTGAAATTTTAAAATGGGTTATTGTTGCAATTTTGATTATAGCAAATTTGTTTTGTGGAATAAGTTTTACATTTTCAAGAAAGGCAGAAGTTATTGAAAAAAGTCGAAATTTGGCAAAAATTAATCTTAATAATTCATTAAATTATTTATTTAAATTACTTAATTTTGAATACAAAAATGAAGAAAAAAATAACAATATTCCATGAATTAATTATTGCAAAAATTTAACTACAAATGGTGAAAATTCTAAAGTTAAAATTTTAAGAAAAGCACTATTAAACAGTGAAATTGGTCAAAACAATACTTGAAAAATTCAAGAAGTAACATTTGAATCAAACGATAAGAACGAAACATTTTTATTAATTGAAGGAAGTCATAAAAATATGACTGAAAATTCATATATTTCATGTGGAAGCAAAAATGAAATTTATGATTATTTAAAAAAGAAAAATAAAGTTAAATTTATTCATCAAAATGATCAAATTCATTTCAAAACTTCAAGTAAAGAAACATATGAACTAACTGACACAATTAAGACTTTTGAAGAATTTAAATTGAATGAAAAAGATTATGGATTTTTAATTGATCCAAAAAATAAAGTGTCTTGCAGTTGAATAAAAATTGAAAATAACTTTTTAAATATTCAAAATAGTTCTGATCCAGTAAAAACAATATTAAATCATCTTTATTTGATTTATGTTTTAACTGAAATAGTAAAACAATTTACAAAATAAGAAGGCATTTCCTCCTTTTTTAATTTTTATTTGAGATAAACAAAATATTTACTTTTCCATATTGTCTTTGTTTTTGTATTGTAAGCTTTTTTGGAATATCAATCATTAAGAAATTGTCAGTTTCTAAAATAACTAAACCATTTTTAGTTAAAAATTTATTATCAACTATCAATTCAAGAGATTTATTAACCGATTGATATTCCTTGTATGGAGCATCAATAAAAATAAAATCAAATTTACGTCCCGAATTCTTTTGTAAATAATTCAAAACATCATCTTTTACAATATCGATATTTTCAGCTTTTAGTTTTAAAGCATTATTTCTTATTGTTTTAATTGCTTCATTATTATTATCAACAGCTATTGCTTTTACCGCATAGTTGCTTAATGCTTCAAAACTAAAAGCACCACTACCTGCAAAAAGATCTAAAATGCAAGCACTTTCTAAGTTAAATCTTATTGATGAAAAAATAGCTTCACGAACACGATCCATAGTTGGTCTTGTTTCCAAAGTTTTAGGTTGTTCTATATTTAAATGACGATATTTTCCACTGATAATTCTTAACATTTTATTCTCCAAAATTAAAATTGTTACTTACATTATATTTAATATTTAATACTTAATATATAATATTATTATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTACATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAATCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAATTAATGAGGTATTTATGAGAACTTATGAAGATAATTGAAAGGCATTAGCAACTAATGGATGATGCGTCTTAATTTCCATATCTGTCTTTTTGATAGTCCTTTTGGTTGCAATTGCTATTGGGGCTAAGAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTCTGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGAAGTGATGAAGAAATCGAAAGATTGCCTAAAGTTATTGACTGATTAAATTATTGTTTCAACGATGAAGCTTCTTTTGATTTACTTAACAATAGTTACAATTATCCAACAGGCGAAACTATTAAATACTACTTTAAAGGTGTATATAGTAATGATCCTTCAAAAGCAACATGAAATACCAAAGTATTAAAATATGGCAATGTTTATTATTACAAAAAGATGAATTTTGGTGGACCAGATAGTTGGTCTCCTGCAACAACAACATATGCAGAATCACTAGAAAACAAATTTGATTTTAAAACAATGTTCTCATGAAATATTTTTAAATGATCTCAAACTTTAAGAACATTATCGAAAATAGATTTAGATTCTAAATTTGAAGTTAGTGACAGCAAAGTAGAAGACTTTACAAAAATCAAAAAAATAAAAAATAAAGATATTCAATTCAAATTAAATGTTGAAAATGAAGTTGTTAGAAATAAAATTAAATCGATTTTTTACAAATTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTTAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAATTTGGTAAATTACTTGCAAAAAGTTGCAAGTTTTTTATTTTTCATACAAAAATTGATGACTTTAATTGTTTAAATTTTTAAAAAATTTATACAAACGTAAAAATTTAAATTTATATTTAGTATTAACATTAAAAATAGTATTATTAATATATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGAGAAATTGTCGATAACTCAATCGATGAGGCTTTAGCTGGTTATGCAAATGAAATTAGTGTAACTTTAACTAAAAATGGATCAGTCAAAGTCATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATCGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGACCAAACTATGAAATTTTTAAACGCGCAAAATTTAGTTATGAAACTATTGCTGAACGTCTTAGGGAAAGTTGTTTTTTAATTGGTGGATTAAGAATTCACTTCAAAGATGAAATAAGTGATCGTGAAACAGAATTTATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGAATTAATGAAAATAAACCATTAGCCAAAAAAATACTTGAAAAAATTAAGAGAGCTTCAGAATCTAGAGCCGCCGCAAGAAAAGCGAGAGTAGAAGCTAGAAGTACAAAAAATGCTTTAAAAGAAAAACAAATTTTAAGTGGTAAATTAACTCCAGCTCAAAGCAAAATACCTGCTGAAAAAGAATTATTTTTAGTCGAAGGGGACTCTGCAGGAGGTAGTGCAAAACTTGGTCGTGATCGTAAATATCAAGCAATTTTACCTTTAAGAGGAAAAGTTATTAATACTGAAAAAGCTCGTCTTTTTGAGATTTTAAAAAATGAAGAAATTGGAACAATAATTAATACAATTGGCGCTGGTGTTGGTGATGAATTTGACCTTAAAAAAGTTCAATACGGAAAAGTTGTTATTATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGAGATGAAAATGAATTAAGAGAACTTTTACAGTTACCTAACTTTAAAAATGCTGAAGTACAACGTTATAAAGGACTTGGTGAAATGAATGCTGATCAATTGTGAGAAACCACAATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGAATTAACAAGAATGTTGAATTTAGTTTAGAAGATGATTTTGAAATATAGAAAGGAGCCATTATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGAAATAAACCTTTTAAAAAATCTGCTCGTGTTGTTGGTGATGTTATTGGTAAATATCACCCACATGGTGATAGTTCAATTTATGAAGCTATGGTTCGTATGGCTCAAGATTGAAAAATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAACAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGAAAAGATTTTGGAGTTCACTTAAGTGACTTTGTTGAATTAGTTTCAGGTGAAGAATTAGTTGGCGCAATTAATGTCACTGACTTTAACATACAAAACTATGTTTGCTTATTTACTAAAGAAGCTCAAGGAAAAAGAGTTGTTTTAAAAGAATTTGAAGTTAGTCGTCCAAACAAAACTTTTACAGCTATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAATACTATAAAACAGTAGTTTTAGAAAAATTGAATAATTTTAAAATGGTTCTTTATGAATCATTTTTTGATTTTATTTTATAATTTTAAAAGAGAAAATTATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGATCATTAAGAACACCAAATGTGTTATTTGATGAAACAAAAAATATAGATTGTGACTTTTTAATTTGTGGTCACTCTCATTTTCCTATTTATTATAAAGATGTTAAAACTCATAAAATTTTGTTAAACCCTGGCTCACCTTCATTGCCACGTTGAAATTCAAAACCAAGTTATGCAATAATTACTATCAAAAATGAAAAAAAATATGAAAATGTGGAAGTTATTTTCAAAGAAGTTAACGAAAAAAGTTAGTCTTTGGTAAAATTTGGGTGATATTTATTATATTTTACTTTTCTTACAATTATTACTAACGAAAGGAACAAATGAGTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGAAGAAGTACAAAACCAATTTATAAAGCTTTAGGACTTGATACGGTTGTAGCTAACTACAATGGTGCACAAATTCACCACCCTGAAGATGAAAACTTTATTCCTTACATCAAATATTTAGACTTAAACGAAATGCTCTATATTTTAGGAGACCCTATAGTTAAAAATGAAATTAGCAATGTTGCTATTGAGGGACCTGGTTGAGTTCAACTTCAACATAGAGATCCTGATTTAGAAAGAGTTTTTGGATTCTCAAGCAGTGCTAAATTTATCGTTGGTTTAAATTTTGAAAAAATTCCTCTTAAACCAACTGGAATTATTTTTGATGTTAAAGAAACCACAGATGTTGAAGCATTAAGAAAATACTTAAGAGCTAAATATGGTGACTTAGGTGAATTCTCATACTGATCAAAAGGTGAAGGTTTAACTCCAGTTTTTGATATTACTAATGTTTCGGTTAATAAAGGTAGAGCACTTTCATTATTAGCTAGATACTATGGAATTGATTTAGAACACACTGTAGCATTTGGTGATGGTTTCAACGATGTGCCTATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTGGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAATTATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGAGCTGACTTTAATCAAATTCAAGGTGATATTTTACTTGCTTATAAAGCAGAAAGTATTAGCCTTGAAGTAGCTTGTGCATCTATTATTGCAAGAGATTATTTTTTATACTTTATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAGTTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAATTAAATTCAACTTTTCTAAAGCAAAAAATAAAAAAATATTATCATTTTTATGCAAATTAGAGACGTGCGGATCTAATTATTTTTTTATTCTTTTTTATTGATTATATATTAATCGAAATTTAATTTTTCTTTTTAGTAAGCAATATTTATTGAGTAATTATTGAGTTAATTTGTTAAAAAAAATTGAAAAATTTAGCACTTTTATGTTGTGAGTGCTAAAAATGTGTTATTATTTATACATATACTAAAACGTATTTGAAAGGAAAATTATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAATTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAAAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGATGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGAGACCATGAAATTGTAGATTGATTAGTTGCTAAAATTAAAAACGATCACAAAATTGACATTCGTGAAAACAAAATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGTGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAGTTAATTTAAAAATAAAAAAGTGCCAAATGCTGGTACTTTTTTATTTTTCAATAACTAAAATAAAGCTTTTATTGAATAAAAGTATACTTTTAATATATAAAATAACCAAAAAAATGAACAAAATTAATTTTTTTAATTTTTTTATGCTTTTTTGCTAGCATTTTACTCTTTTAGAATAAAATAAGAATATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATCAGCATTTCATAAATCATTAAGAGGAAGTGATGTTGATGCAGCACTTTATTATGGAGCTTTAATCTTAAAAACAGGCGACTACATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAACATGAATGATATTAAATGAGAAGAAATCAAAACATTTGAAGATTTAAAATTAGCTAAAAGCAAAGTATATTCAAAGGACTCTGAAATAGTTCAATTACAAACAAAAATTAGAACAGCTTCGCCTGAAGAGAAAAAAGAAATAGGTCAAAAAATTGCAACTTTAAGAAACTTTTATGAAGAAAAATTCAAAGAAATTGAATTTAAAATTGAAGCCGATAAAATCAAAAAAATAATTGAAAGCCAATATGTTGATGTAACTAAACCAGTTGACAATCCTGGTTCATTGCACCCAATTACAATAGTAGAAAATAGACTTAGAGATTGATTTATTCAAAACGGATATTTTGAATCAAATGAAAGTGAAATTGTTAGCGATGTTTATAATTTTCAAAAATTAAACATTCCTGAAGATCACCCTGCTCGTGCTATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGTAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCGACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGAACACTAAAATCACTTCTTTCTTATGTGTTTGAAGAAGAAGTTGAACTAAGACTCAGACCAAGTTATTTCCCTTTTACTGAACCAAGTGTTGAAGTCGACGTTTATTATAAAAATGGTTGAATCGAAGTTTTAGGTGCTGGTATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATATAACATCAAGGATGTTAGAGAATTTTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAGCTTCCAAATATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCCTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGAGCAAAACAAGGAGTTTTATTAATTAATACTTGTTTAAGTGTTAATGAAAATCAACCATTAAGCCATAAGAATTTTGGCTGAGAAATATTTGTTATTAATTTAATTAAATACATTTTGAAAAAAAATAAAAATGTCATATTTGGCATCTGAGGAAATGATGCAAAAAGAATATTGCAAATTGTTCAAAATGACTATAAAATAGATGAAAACAAAATTATTTTTACTTCTCACCCTTCCCCTCTTGCATATTCAAAAACAACTAATAGTTTTAAAGATTCAAAGTTTTTTATAAGAGTAAATTCATTATTAAAAAATCCAATTAATTGAGATTTAAGAAAGGATTAATATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATCAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAAAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGTGACTTTGTATTTCAAGATAAAGAAATGGCAGGTGTTGTTTCTCAAGGCATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGATTTGATTACAAGAAAAACTACAAAACTGGAATTCACAAACAAATTCAAAAAGTTGATAATGGTGAAGCGAATGCTTTAAGTTTTCTAGAATGCAGATTCAAACACAAAGACACTAAAATCGAAGATATGTTATTTTTAGCAAAACATAATGTAGAAGCTAAAAATGTTTGAGCAATAGATGTTACAAATTTAAACTTAATTTATACTGGTGCTCCAACTCATGTTTATGATCGCAGCGAAATAGGCGAAGAATTAACTTGCAAAAAATATAGTGGCAAAGTTACTATTTTAGGTAATAAAGAAATTGAAGTAAAAAATGCTTTAGCTATTTATTCAAAAGATAAAGTAGTTTCATTAGCTTCAGTTATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGATTAGGAAAAGTACACCCTTCATATAATAGTGTAGATGCTTGAGTTTGTGAAGTACATAAAGAAGTAAATAAAGGCTATAAATTATTTGAAAATTATGATTCAACACCATTTAAATCAATTGACTTAACTTTCGAATTAAAACCACATCAATCAATTGATGATAAAATTATTGAAATCAAAAAAGTAGCTTCTATTTTTGGAATTTATCAAATTGATGAATTTGCAAAACCGGAATCAAGAAATGTTACTTTAAGAATTATTGGAAGCAATGAAGAAATTCAAAAAATCAATAATGAATTCAATAATTAGGAGGCATTATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCAGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAGCCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGAGCAAAATTTAGAGAAATTGCAGATGCATGCGGTGCTTACTTGATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTACGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGAGTATTTCCTGGATATCAAGGTGGGCCTCTATTCCATACAATAGCAGGTAAAGCTGTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGAAGAGAGCTAGCTGATATTATTGATACTATCTTAAAGAATCATGAAACTATTCTAAAACCAGAAAGTGAAAAGTTATTTAATAGCCTTAAAAAGAAGGTTAGAGATTTCACTAAAAAATTCCCAATCATTACTGTTTATAGATAAAGACGTCAAAAATGCATCTTTATCTTTTTATAAAAAAACTTGCTAAATAGCAAGTTTAATTTTGCATTTGAAGAACAGGCTGATCTTTATTACTATCATCTTCTTGTTCAAGTTGTTTTTTATATTCTTCTTCAAGTTTTGCGGCTTCTGCAAAGAATTTGTGTTCAAGTTTTCTACTTCAAGTAAGTTTAGCTTGATCAATTTTAGGTATAACTTTAGAGACATAAATGTAGCTTCAAATTAATAAAATTATTGAAGCGACTAAAGCATTAGCAATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGAACATTAAGCGCAATTAATCATAAGTTATCAGCTGTAGCAGCTACAAAACTGTAAGCCAAAATTGAATAGATAACTGCAGCTAACATAGTTCAAAGATAAACTTCTTTGCATCTTTTAAAGTTTCTTCGCGTATAGTTATATGACATTAAGCTTCTAGCTCCATCATTAATACCAAAAATAGAAACTAAGAATAATGAAACAATCGGCATACCTTTTGACCAATAATATTGGAATGAAAGTGGTTCTGCAAAAGGGGTTTTGGTTAAGAAAATGTTCATAACAGCAAATGAAATAGCAACACCAAATGATCTTATGAAACCACTAAAACCTAAGGCAAAAGTAGGCAACAATAATTTTTTATTAAATTTAAATTTGAATAAGTGTCCAAGCGAAAGTCAGGTTTTATTCCGCTTGCTATTAATTACCACATAAGCTAAGTAGGCAGTTAAGTTAATTGACCAACCAATAATAGTAGCAACAACACCACCTAATACACCCATTTTACATGGCACAATAAATACTCAGTCTAAAACAACATTTACAATGTTTGCTCCAATAACTCAGCCCATAACATAAGCGTTATAGCCTTCTGCTCTAATAAAGTAAGATAAAAGCATTGAAAGACCTTGAAGTATTGTTCCTGAGGCATAAATATAAACATATTGTTTTGCTCAATGAACACTAAGATGTCTTGAAGCATCATAAAATGCTTGTAGTGTTTCATTGTCTTCTTTAGTCATTAAGCTTAAATTTCTAATATTAGTTTTACCTGCAAAAGCATCAACAAGACCCGATCATATAAAGATAAATGACATTAAGGTTGCTAAGAATGATAAAGCTACTGTCATTCAAAACATAGTTGATCACAAGTCTCTTCCGCTTTTTTCTAATTTTTTAGAAATACATTTTGTGTAATAAATACTTGCACCAACAGGTACTAAAAATACTATTGAGTTAGAAAAAATAATTAAAGGTTGAATTGCAGCTGTGGTCATTGAAACAACCCCATTAGCATCAATTTTTCCTAAATTCAGATCTTTATAACTGGAGTTATTTATGATATCCAAGTATTTCTGCTTCAATTCGTTTGAAGTTAAAGAGTTTAAAGTATCTGGTAAAAATTGAATCATTTCGCTTCAAATATTTCTATTATCAGGAACAAATTTTTGTAAGAAAACTTGATCAATAAAAGTATATAAACCAAACATTAAACTACTTAATAAAGAGGGAATGGCAACTATTCAAATAGCTTTCTTTATTGGAGTATTTGCAAATAATTTTTCTGCTCTTTCCTGTTTAGTTGTTTTTGACATTTCAACCTCCTTTATATGAAAAATAAAAATTCATAATGGAAAAATCATACCATTATAGTCATTCTATTAAAAATAAAATTGGTGTTTAATAAAACAAATGGTGCCGACAACTGGACTTGAACCAGCGACCCCATCCTTACCATGGATGTGCTCTACCGACTGAGCTATGACGGCTAATTATGTTTTTTATTATAACAAAAATCATAGTATTTTTAAAAAAATAATAAATATTTATTGGGTAAATTTTTAAAAATATTTGCTATGTATTTCAATATTTTTAAAATTAATATTTTTACGAAAATCACTGTTTTATAAGCTTTTTTGTAAAATTAATCAATCAAAATTTTAATTAACATTTTATATATATAAAAGTTATTTAAAGAAAATTTAGCACTCTCGATATAAATGTGCTAAAATAGAAATATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAACTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTCAGCAAGTTTACTCAAAACATTTTTGAAACAGTATTCAATTTTGGAGGCGGAACCTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGTGATGATATTTACACTGAAATCGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTCGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATATTAGAACTTGTAATGTTTGTAAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAATATAAGCCCTAGCGGCTTTTTTTATATAATTAAATCATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGAATCGATTTAAAAAATAAAGTTGATTCATATGACACTCAAAATTTTGATTTATCAAACAATTTTTTCAAGAAAATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAACAAAAGTTGTTTTTTTATTTTTCTTTTATATATAAAAGCCTTTTGATTTTTAAGGAAAAAAGTTGCTTTTGTAATTAAATTTAAGTATAATACTAAATATTAAAATATAATTAGAACTATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGAGCAATAAAAGAAGCAATGTTTAAAGTTGACAATTCTTTGTTCGAATTTAAAAACATTAAACTTCAAAAAGAAAACGAAAGTTGACAACATAAAAATTTTGATATTTCAATTTCACATGAAGATGACTATTTAGTAGCTGTGGTTTTGAAAAAGAAGGAGTAATATGAATTATCATTTAAAAATGTTTCTTACATGGTGAATATGATTATGAAGGATTTATCGGATTAGTGTTTATGCGCGTAAATATCGTCATACTCCTGATTACTATACACCACAACAAAGATACGATTGATTACTTAAAAAAGTTAAAAAATATTTAAAGCTATACAACATTAAAGTTACTGTTGAAGGTTATGACAATTTACCTAAAGCGCCTTGTTTATTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGAAAGAAACAAGAATTCAAAGTTAAGTTCTTGAATCCAATTAAACCAAGTACATTTATGTCAATGGATCCAGGAGCTTTAGCAGATAGAGTAAAAAGTCTTATGGAAAGGGAGCTAGAAAATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGACATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACAAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAATTTAATTAAGGGTGCTATTTTTAGCACCTTTATTAATATTTAAGAAAGACAAAATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTATCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGCAACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACTAGCTTTTTTGAAAAAATATATAAACTAAAAAGTGATTTTAAAGAAGCTAAAAAACTTGTAGAAATTAATATTCAACACGATGAAAAATTATATAAAATGGTTGTTACAAATAATACTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATAAAATTTTTGCATCTATTTTAACAGGCAAAAAACATCAAATTAGATTAAGTATGGCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGGGGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGATAAGGAGAATTATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGATGGAATTATGGCTGAATGAAAAGTTTTAAATAAAAACTTAAGTATGCTTAAAAAATTAGATACAACAAATGTTAAACCATTAAGCCACATTAATGAAGAAACTTTAGTTGACTTTTTAAGAGAAGATGTTGAAGACACTTCTTTTAGTATCAATAAACAAACTGCATTAAACAATGCTAAGGACAAAGATCAAGATTATATTATTACAACAAAGGTGGTGAAATAATGAAACTACAAGTTTTAGGTGATTTTGATTTAGCTCAAGATGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGTTTTAAAAGAAGGCAATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCCACTAAAGATGCAAAAACAACAGCTTCAAGCTTGATTTTAGAAAATTTCAAACCTGGTTACAATGCAACTTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAAAAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGAGAAGAAATTATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGAGATAAATTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGAATTAAAAAAGATAACTTACCAATTAATTTAGCTATTGATGCCGATATTTATCAAGATGAAAAACTTCTAAGTCATGCTCTTTGAATGGAAGAACTTTTAGGAGGCAAACATGAATAATTTTGAAGTAATAATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATGATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGAGAAAGAGAAGCTAGATACAAAAAAGAAAATATTCAAGATATTTACATCAACAGTTTATTAAATGATCTTGATTTAGCAAAATATTTTGATTCTATTAATTATGCTGATAGAGATAAATTATCTAAATTATTTTTTTCTGAAATAGTTTCACTAGCAAATAGTAAAAGCATTCATCCAACTGAATTAAATATTAAAAATGGACACTTATTAAAAGGAATTGAATTAATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGAGTTAATGAAATTATTAGCCAAAATGGAAGTGTTATTGCAGAATATCCTAATCGTGAAGAAAGAGTTCTTAAATTTGTTTTAGGTGCTTTAATGAAAGTTTCAGGCGGACAAGTAAGTCCAATTAAAGCAAATGAAATTTCAGTTAGAATTCTTAAAAACAAATTTAAATAATCAATATAACAACCTTTTGAATGTTTATTAACAATCAAAAGGTGTTTTTTTAATTGTTTTGTGTGTTTCACACATAAAAATGTTATAATGTATGTGTAAAAATCACAAAAACACAAAAAGGAGAAGAAAATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAATATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGAAGAACAACAGTTGATTTAGATTTTATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACAGAACCAATAAATGTTAATAATGAAAAGAAAGGAATTAGATTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGAAATTCAAGATTAAAAGACTTTTTTGATATTTATTATTTATTTGAAAATCAAAAAAAATTTATTAATAAAGAAATCTTAAAAAAGGCCATTGACAATGCTTTTAAAGTTAGAAATGCTTCATTTGATAAAACTATTTTTATAGATTTACTAAATGCACTTAAAATTTCAGAATTACAAAATTTACAATGAGAGAAACTTGTTTTATCTAAAAATTGACAAACATCAAAAAGCTTTGGTGAAATTGTAGAAGTAATAATTAAACATTTTGAGAATGATATTGAGAATGTTTAGAATTTTGTGTAAAGTATTTCACTTTCTTAATAGAAAGGAATTGATTCAACACAAAATTTTTTATAGCCCCCCCATTTTTTAAATTCTAGAATTTAGGGTTTGTTTATTATTGAATTATTTAAGATTTTTAAGGACTCCATTTTTTAGATCATTGATGCTATAAAGATGTTCGATGATTTCGAAAGCTTCTTTCAAATTGTTTTTTGCATTTTTTCAACCATTTATTCCATCGGTAATTCAAATGAATTCAAAATTAGTAATTGTTTTTGTAGATAAATAAATTTTTTCATAGCTTCTTGAAACTTCATTAAGTTTAGAACCTTGAGCCGTATAAAAATTTACTTCAATTGCATAAAATTTATGATTTTTAATGATTCCAAAATCAAATCTTTTATTTGCAGTTGTTTCTTGAATATTATTTAAATTAATTCCAAACTTTTCATAGATCTCACTTGAATATATTTGTTTAAAATATGTTTTGTTTTCAACATATCCAGCTTGTTGAATATATTTTTCAACTAGTTTTTCCATTTTAAAACCTGTTCTGTTTTTTCTTGCATTGGTGTTGCTGCCTGCTTGAACACCATCATAATAACCTAAAACATAATCTGGACTTTTATTTTTAAGTCAATCTTTTAGACCTGTTTTTTCAAAAAAGTCATTGTAATCTTTAAAGTCATTTGTAAAATTCTTAAAGTCTAATTCTTTTATTTGCTGATTATCATAAATTTGCAAATAATTATTTCTTACAGCTAATATACTTGGCAATATTTTTAAAACTTGTTTATCATTATCATATATTTTTTTAAATTTTTCAGAAATATTCTTGTCACCTACAATATTGCTGAATTTAACTAGTTCTTTTCTATAAGCTTGTAAATCTTGATTAATTTTTTCAAAATCTACATAATAGTCATAGCCAACTATTGTTGGTGTTAAATTATTAAATCACTTTTCATTTTACTCCCTATAATTTGTAATTATTACTTCTTCAACTTCTCCTCGTCCACTTCCATTTGAATTTATCATTCTTTTTGCTTTAACAACATTAATATTAAAGTCTGCATATAATTCATTAATATATTTTGTGTTGTGATTACTTAACATTACTTTGACACCTTTTTTGTCAAGTTTTTTAAAAACTTCGGATAATCGTTTTTGTTCTTCTTTTCCAAAACTTTCTGAATTATATGAAGTGAAAGTATTTTTGTTTTCAATGACATCATAAGGAGGATCAAAATAAACAAAATCTCCTTTTTGAGCTTTTTCACAAAATACTTCAAAATCTTCACAGAAAATTTGATTATTTGTAGTTTTTAAAAAGTTTTTTAAATTTTCAAAATTGTCTCTATCAAAGCAATTAACTGAGTTTTTCTTGCCAGAAGGTACATTAAATTGTCCTTTAGAATTTACTCGGTATAAACCATTAAAACATGCTTTATTTAAGTATATTGTTCGGGCAGCTTTTTTGTAATTTGGCAGTTTATCGTATTTATCTAATTTGTCCAAATCTCTTATTTTATAGTAATACCCATCATTATGATTGTTTTCATGATTAATTAATTCTTTTTTTAATAATTTAAAATCTTTATCATTTTTAAAGCAATTAAAAACATTAATTAATTCTTTATTAAAATCATTTATTATGAAATTTTGCGGCTTTAGATTTAGTAATAATGCTCCGCCACCAACAAAAGGTTCCAAATAATTGTTATATGTTTTTGGCATTAAACTTTTAAGTTTATCTAATAATTGAGTTTTGTCACCAGCCCATTTTACAAATGGTGAAAGTTTTGTGTTTAATTTTCCAATATAAATATTATTCAAATTCAAAGCATTATATATTTTGCAAATTGTAAAAAACTGAACGCCCTTAATTTGACCTTTTTCAATTTTAGTTAATTGAGATCTTTCGGTGCTACTTAATTCTGCTAATTCTTGTTGTGTCATATTTTTTGCAAGTCTTTCCATTTTAATATTGTCGCCAATTGTTTTAAAAAGTGTTTCCATTTTGTGCTCCTTAAATAAATATATTTTAATTATATCAAATGTGTTGTTTTGTACACAAATTATTTCACTTTTTATTTTAAAATTTTTCAATATCCTCTTGTCTTACCAATTCTAGCTATGTAGCCCTTGTCTTTTAAAAGATCTAAAATTCTTTGAATAGTTCTCGAAGATTTTTTTATTTTTTTAGCTAGTGAACCAGCTGTTTCTTTTGGATTATTTTTTAAAGCCATATATACATAATAAGCAGAATCTGGCAATCAGTATTCTTTAAATTCTTCATCTTTACTAATTAAACTTTTGTTTTCTCTTAAAAATATAAATGCAAAACCATAATTGCCTTTTTGATAAGTAATTTTAACATTAGTTTTTTCACATTCTTCATATACTCTCTTGAAACCATTATTACAAATTTTAGCTTCATCAACTAAATTAATCATTTTTAAAATTAATGGATTTCTAATAATTGATTCTATGTTTTTATATGCAAAATCTTCTGGAGTGTATTCAATTGGAAATTGACCAGGATTATAGATTTTAATATTACTAGGATGAATATTAATTTCGTGTTCTATTGCAGATTCATATTTTGCATGTGCAAAACTATTAATAATTATTTCCTTTAAAGAATCAATTGGTATTTCTGGAATATCAATTCTTTCTATATCTACAAATTCTGCTTTTCAATTGATTTTAGATTTAACATATTCATTAGCTTTCTCAATTAATTGAAAGATGTTACCAGAAATTCTATTGATATCTAAAAATGTTAATTTTTGATCTGTTGCAAAAACAGCCATTTTTAAAACTATAGGTTCTCTATGCGAAAAAAGATAATGAGCAGCATTTGTTAGTTTTTCATTTTTCATCAAACTTAATTTATTTAAAATGTTTTCAGCATTAAATTCTATTTCATTTAATTTTTTACTTTTTATAGCTTTTTTATAAAACTTTTGAAATGTTTCTAAATCAATATCATCAATTTCATAATCGCTTAATTCACTATCTCAGCTTTTGTTTTTATTGAATTCAAAAAGCTGTCTTAATTGATTAGGACTGAGTATTTTATCCTCATCATCATTTCTAATATAATAGATGCCTTCAACTGAATATGGAGCATCATTCCCACAAAAAGTTACCTCTATTATTTGTTTATTATCAACAATTTTTTTCTCAATAATAGGACTAATTACAGGTTCAATATTTTCATAAATTTTTCTGCAAATATCTCTTAAAGTATTTATGTTGATTTTTTGATCATATACTAATCCATCATCTCCGATGCCAAAATATAATGTGCCTTTTCCGTGTTTATTTAACATGGCACAAATTGATTTCATAGCCTCATTTATTTCATTTGTATTTTTCTTAAATTCAAGTGTTTCGCTTTCGATTCCAAAATTCATTTTTAACCTTTTTTTAACTTTTATAATTACATTATACATAAAAAATTTATTTAAGACAAATTTATGACAAAATTTATGACAAAAAAATAAAAAAATATTTGTTCTTATTGTGATAAAATTAAAATAATTAACATAAAAAGGGACAAAAATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTGATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGAGAAAATATAACAAATAAAATTAGCGAAGAAATGGGTAGTGAAGCTGTTAGATTTTTTAAAAATATTGAAAAACTAAAAATATGAAAAGATGATCCTAGATTAAAAAATGAACGACGTGGAATTATTGATGCTATTGAAGAATATGAACACAAATTAAGTGATGAAGTTGAAGAATACTTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACATGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGAGTTGACTATATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGATGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGAGCTAACTACGAATATGATTTATATAATGCTATCGAAAAAGATCAAGCTAGTCCCTCATATACTTCATTAAGTAAGATTTATCATAAGAATTCTATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGAATTAAACTTGGAAAAAAGATATTTAAAGTTAAATAATTTAGTGAGCAAAACATGCTCATTTTTTATTCAAAATTATTGAAAAAATCAAATTTAACATTTTTTATATCGAAAAGTGTAAATTTTGTATTTTTACAACTCAAAATGTTGAAGCAAAAGACAAAATAACTTATTATCCAATTTACATGTCGATGTTTTTAAATGATCAAAATATAAAACTTCCTAAAAATGAAAAACATCAAGATTTAGGCGTTTTAACCCAATATTTATGATTTTTTATTTGAAATATGTTGCATATTTTCTCAATAAAGCTCATTATAAGCAATTTGTCTTATAATTATTTTTATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGATTTTTGGGATCTGCCACTGTGCCTTATTTAGGTTACATTCCTATTGGGCATACTCAAATAACCTTTTTAGCTTTTATAGTTGTTTTTGGAACTTTTCACTTAGATTATGTTGGTAATCTTTCAGCAGGATTATGTTTTGGATTTAGCTCATGAATTTTAGCTTTTATAATGGGGGCGATTAAATATCAAAACTTTGATATCGCCGTTATTCCTAGAGTTTTAATGGCAATATGTGTTTATATGCTGTATGAAATTTTTAATGTTAAGAACAATATAAAATGTTGAAAATTTATTATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATCAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGAATAATTGTTCATCCAATTAATATTATTGGCGAACCTATAATTTGTGGAATGTTAGCCTATTTCACTTTTCCCTTAATAGTATTTTTAAGAAGAAGATTCGAACAAAGATTCAGATATCATTGATAAAAAATATTAAAATATAGATCTAAAATATCGTGAAAAACGGTATTTTTTTAAATACCTAAATTTTTAAACTAGTAAAAAAATGTTTATTAAATTTAATTAATATATTAAAATTAAAATACATTATTAGTTAACAAATAAAATTTAAGGAGAATTATGGCTGAAAATAACAAGAAGTTTACTATTGATGAAAAAAAGAGTGAATTAGTTTACACTCTTGAAATTAATGGCAAAGAATGAGAAAAACTTTACGCTGATGCAAAAGCCAAGGAAGTTAAAAACATTAAAATTCCTGGATTTAGACCAGGTAAAGCACCACAACATGAAATTGAAAAAAGAGTTAATCCTCTTTCTGTAGCAAGTGAAGTAGTTGATCAATACTGAAGAAATAATCACGATCGCTTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGAATCGCTCAACAATTAGAAAAGAATGCTTTATTATTACCACTTAAAAAAGATGAAAAAACAAAAGATGGTGACACTGTAACAATCGATTACAAAGGTTTTATTAAAGATGAACCATTTGATGGTGGTGAAGCTCAAGGTTTTGATCTTAAATTAGGATCACACACATTTATTGATACATTTGAAGATCAATTAATTGGCAAAAAAATTGGTTGAAAAGGTGAAGTTAAAGTTACTTTCCCTAAAGAATATGCAGTTGAAAAACTTAAAGGACAACCCGCTACTTTTGAAGTAGAAATTAAAGATGCAAAAAGAAGAGAAAAAGTTGAATTAACTGACAAAAACTTTGGTACAGTTCGTCTAGCAAAAATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTGTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAATAATTACATATAGGCACATAATAATTAATCAGGCAATTTGCTTGATTTTTTTATTAAACTTTAATTACTTATTTACTAATAACAATATTTATAATAAAATTAATTTATTAATTTAAGGAGAAAAAAATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCATATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGAATAATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGCTAAGTCTATATGAAGTTCAAAAAGGAAAGGATTTCTTAATCAAATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAATAAGAGGGTTTATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGAAATTATGTTAAGCAATTAGAAACTGAAATTGAATCATTAGAAGAAGCTAGAAATGTTGATCGACAAAAATTAAATAACAAAATAACTGAATTGGAAAACAAACAATTTAATGAATTACTTGATACAAACTTAAGAAAGAAAATCAAAAAAAATGAATAATAATGAGCAAACTTTAATTAATTGATATCCTGGTCACATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGACTTGATTTAAGACAACTAAAAAGTAGAAATCAAATCTTAAATCAATTAAAAGTTTTATCTCAATCTAAGATTAAAAAAGATAAAGAAAAAGGTTTTTTAACTAGCAACATAAAAGTTTTTGTATTAGGTGTGCCAAATGCTGGGAAAAGTACATTGATTAATTTAATGAGTCAAAGGAAAAGTTTAAAAGTAGCAAATTATCCTGGAGTTACCAGAGTTAAAAAATGAGTTATATCAGATAATTTTTACTTTATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGAATCAAAAACTTAAAGAATGTTACTTTTGATTAGGAGAAAGTTATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTAAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGACAAAATAAAGCGGCACCATCAATCACATATACAACTTTACTTTGAGTTTTAATTTGTGTCTTTTCAATACTCACAATTCTTTCTCTTTATTTATTAATTTTATTTTTCATTGAATATGGTTTAATCAAAAAAATAGGTCTTAAAAAATCAGAACAAGAAATAGAAGCATCTATAAGAAAATTTGTTAAATTTGGATTTAAAAAATATCCTAAAAAACAAATGGAAATGCTTGAAAAATTCTAAAAAGCCTGTTTTATAGTGCTTTGGTAAAAATTGGCAAAAAAGAAAAATTCCACAAAACAATCATATGATTGTTTTTTTAACAATCTTTAAAGTATAATTTAAATATAAAAAACAAAGGGGGATTTATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTCAAAACATTGCATTATTAGGATCAAATGGAGCTGGTAAAACCACAACAGTTGAAATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAATTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGCCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATCAACTTTATTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTTGCAGCTTGCTTAGAGTACTACATTTAAGGAGAATCATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGAAAAGCATTTGTTGGTCCTTTCTTTGCTTTTGGTTATCCTATAGTTTTTGTAATTATCTTAGGTGCTGTCTTTAATTATGAAATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTTGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTGGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGGTATTTGAACAGCCTTGGTTGCTTTAATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGAGCTGGTTATATTTATAGTGCTATTGTTTTAACACTTGTAAGTATTGCTGTTGGTTTATTTATAGTTTCAGTAAGTAAAAGTATTTTAACTATCCAAGCAATAGGTTCATCATTATTAATTATTTCAATGTTTTTAACTGGACAAGTGTTGCCTATATCTCAAATTATTGGCCAAAAAGTAATGTGATATTTATCATACATAACTCCATTTAAATCGCCAATAACTCAAAACACAATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGGAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGACTTTGAATAGTTGTTTTAGTTGGCTTATCTCAAAAATCATTTACTTGAAATACAAGATAATAAATTATTACATGCTCAGTCATGTAATTTTTTATTATAAAAAATTGGCTTAAACCAATTATTTTATCTTTACTATTTCTTCATTTAATGATTTAAACATTTCATAGCATTTTTAAATATGCAGCAAAACCTTCTTTAATCTTTTTTTCAGCAATTTTGACTTTATAGACTAAATCCATATATTTATTAGCTGTTGTATGGTTTCTTTTTTCAAGATCTTCTTTATCATTAGTTAAATCTATTTGAATTTCAAGTTTATATTTTTTAAACAAATTTTGAACTATTTCTTTTGTTAGTAGTTTTCAATTTTCATTAAATTCTTGTTTAATAAAAGGAAAAACCTTTTTGTTATTATACAAAGCTTGATTTTTTTAAAGCTGCTTATATTTATCAATTTCTTTAGCAGGCTTGTCATACATTCTGTCTTCTTTAATGCATGAAGCACTGGTTAAAAGTAAGGTTGCTAAAGTACTTAATGCACTATTCATTCTTTTCCTTATATATTAATATTATAAAAAGAAAACTTGAGCAATGCTAAATGTGAGAATAATTATCAATTTGTTGCATTAAAACTTTATAATTAAACTAATAAAAAAAGGAGGTTAGTTATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAAGCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAAGGAGTTAATTATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGGCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGCGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAGATTTCCGAATAACACTGTTTTATAGTTTTATTAGTTAAAAATGAAATAAGCATTTGAAAGTTTATTTTAATAGCAATATTAGTTGCTTTTTTAAATATAACTTTAACCTTAAATTTCTAACATTTACATAACAAAATATTTGTTAATTTAAAAAAAATATTACCAATCAATAATTCGCAATAATTCACTCAATTAACATTATTGGTTAGCAAATAATTTTTGCAGTAGTAGAATTTGCTTATCCATTGGCTAAATTTTTAGGAATATAATTTGTGCAAAGATTAGAATTTCATTGATAAAAATAAAGTTATTGTTTATAATGTTAAAAACTAATGGAGGAAAATTATGAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGAGCTAAAGAATTTTTATCAAAAGACTGAGATAAAAGCAAGAATACAAAAAAAAGTAATTATTTTTAATTTAAAAAGATTTTTAAAATGAACAACTGAAGAATTAAATAAATATCAATAAATAAAAATTGAATTTTAAAAACCGGTATACAATGATACCAGTTTTTAAATTTTTTTGCCAAATATACTACGTTTAGAAACAGTGTATGAAGACACATAAGTTTTGTAATATGCTTCATTAACTATAATTCCTAAAAACATAAAATATGTTAAGAATGAAACAAATAATGCAATATACATAAATGAGCCTATAATACCATATTTGTCATAGTTAATTAATGATGTTAAAAATCCAAAAATAGTGATAAAGATCATGACTGGAAAGCTAGTAATTAAAACGCCAGGAAATACTGTAGTTCAACTTAATTTAAAGCTAGGACTCATGGTATAAAGTAAAGTTAAGCCAAAGTAAAGCATTATTAATAAATAGAAAGAAAATGTTATATAAAAATATATATTTTTGTGATATGTTGACATATTGTAAGTAAAAGTTTGATAGAAGAATAGATATAAAGCAGCTGCTAAAAATAAGTAAATACTGATACCTAAAACAACAAATAATCCTTTAATTCTATTAGTAAATCAGTTACCTAAATTTTCATGTCCATAAATATAATTTTGACTGTAAATAAATTTGCCAAAACCACTTGAAGCCATTCATAAAGAAGCCAAACAAAGAATAATTAAAGAAGTTAATTGAGCGCCTTTTTTTGTGGTGTCAGGGATCTTAATAATAGAAGTAACACCAGGAATAATTCGAGTTAAAACTTCATTAATAAATAAAGTATGATAACCAGGAATTAACGAAAGTAAAACAATCATAATTGTAATAATAGGAACAAAGGAAACTAAGAAATAAAAAGCAGTTGAAAGTGGAATAAAAACAAATGATTTATCAGTAAATTTTTTGTAAGTTCTATTAATTAATTCGTTTGTTTTGCTTTTGTTTTTTCAACTTAATCTAGGGGTGGCTATTCACAAAATAATCATGATAAAAAACTTAACTATTTTTTCAAAAAATAGTCATTTTTTATTTTCTACAGGCACAATATTACGAGCAAATCAAGATTTTTTTAGACTGTTTCGGTATTCTTTTTTAAGTCTATTTTGATCTAATCCTGTATAAATTTTATTTTGATTATTATTATTTTGCATATTTATAAGATTATATATTAATTAAATTAAGCATAAAGATTAATTTAAATTATTCTAAACCACTTTTGTTTTTGAAAACTAAATTAATTGGACAACCAGTGTAGTCTAAAACTTGTCTAATTTGCTTTTCTAAAAATCTTTCATAGCTAAAGTGGACATATTTTTTATTGTTAACAAAGAAAACAAAAGTAGGAACTGGATCTTGAGTTTTACGAGCAAAATAAATATTTAAACGGCCACCATTATAAGGAGGAGCTGGCTGAATCATTTGTGTTTCTCTGATTAAGTTAGAAAGAACTGAAGGTTTAACATCACGTTCAAGATTTTGTTTAACTTTAATTATTGTGTCAATTAATTTGTCCATTCTTGAGTTTGTTTTAACTGAAACAAAACAAATAGGAACCCAAGGTACAAAATGAAATTTGTTACGCATTTTCTTTTCGAATTCAACCATTGTTTTAGTTTCTTTATTAATTAAGTCTCATTTATTAACCACAATAATTATTGGTTTATTGTTGTCTAAAGCATAGCCTATAATTCTAGAATCGAAGTGTGATAATTCTTGAGTTGAGTCAATTAAAACTAAAGATAAGTTAGATTCGTCAAGTGAACTCATAGCGCGTTTTAAAGCATAATGGTCAACGCTATCTTCAATTTTACTTTTTCTAGTAATACCAGCTGTATCAATGATTTCAAAAACTTCATCTTTGATTTTTACAATACTTTTAACACTATCTCTAGTAGTTCCCGCAATATCAGAAACTATTGAACGGTTTTCATTTGCTAATCTATTTAACATTGAACTTTTACCTGAATTAGGACGTCCAATAATTGAAAGTTTGAATAGTTTGTCACTCTTTTCATCATCAAGTCTAAGATGTTTAAGACATTGGTCTAAGACATCACCAACACCATTACCATGTTGAGCTGAAATAGGAAAAATATGATCCACGCCAAGTGAGTATCATGAATAATTAAACTGTGAAATATTATCTAATTTGTTTGCAACTACGACAATAGGTTTGCCAGATTTTTGAAGCATACTGTAAATCATTAAATCGTCATTAGTGATGTCAGAAGCACCATCAACCATAAAAATAATTACATTAGCTTCTTCAATAGCAATTTTTGCTTGAATTTGAATATGTTCTTGAAAAGGTCTATTTTCGATTTCGATACCACCAGTATCGATAACTTTAATTTCTTTTCCATTTCAAGAAAAGGTTTCATAAAGACGGTCCCTTGTTACTCCAGGACGGTCATAAATTATTGAACTTCTTTTACCAATTAGCCTATTAAATAGGGTACTTTTACCAACATTTGGTTTTCCAATAATGGCTATAACGTTGTTTCTCATTGATTATTTATTAAGTTTTTCTATTGTTTTTTTAACTAGTTCAATAATTTCAACAACAACTGCTTCAAAACCCATATTTGTACAGTCTATTTCTTTTGCTTCTTTAACTTTATGAAGAGGGTCTACTTCACGATTCATGTCTTGATAGTCTCTTAATTTAACTTCTTCAAGAACTTTGTTGTAGTCCATATTAAATCCTAAAGCAATGTTTTGGTTTAATCTTCTCATTGCTCTTTCTTCTGGTGTTGCTGTTAAAAAGATTTTTAATTCTGCATAAGGCATAATTTTGAATGTAGTATCTCTTCCATCCATAATGTAACCTTTACCTTTTTTAGTAATATTTTGAATTAAATTAACAACATATTGACGTACTTTTTTATATGTTGCAATAGTTGAAGATCCATAAGAAATTGAATCTTCTCTTATTTCAACAGAAACATTTTTTTCTCTTAAAAAGATGTTTTCTCTTTCATCTAAAGTGATGATATCTTTTTCTAAAGTTTTATTGATTTCTTCTTCATTATTTCAATCAACACCTAATAATTTTGCATTTAAAGCTAATGCTCTATACACACTTCCACTATTAATAAATGTGTATCCAAGCCTTCTTGCTACTTCTTGTGAAACAGTTGATTTACCTGCACCGCAAGGTCCATCAATTGCAATATTAATCTTTGTAATTTTTGTCATAGTGCTCCTTTAACTAATAAAATATATTTATATTTTAATATATAAATAAGAAATAATAAAAAAATCACGTAAAGTGATTTAAAAATTATTATTTGTATTCATTAATTAATAGCACTGTATCATTTATAATTTGCTTCATATTGTCAATAGGTTCAAGAAAATTTCTTTTAAAATTTTTATATTTTTCTTCAGCTTTTTCAGTTCCTTTTTTGTCTTTTTCATAGGGCAATTGGGAATTTTTGATTAATTTTTCTAATCTATCTACAAAGGTATATAAATCAACACTTTGAAGTTTATAATTTGAGTTAAAAACAATCATAATATTTTTTGCCTTATATTCTCATGAATTATCAGTTATAAATTTTTTAAAATCATTAATTCATTCATTTAATTTATTAATTCTGTTGTTTTTATCTTTAGGAGAAATAACTGTTGATTTTCATTCTCTTAATAAATTTATTGCAGCATGAGTATGTTTTCCATCACTCTCTGAAGAAGGATTTTTAATTGTGCTATAGTCAATTGACGCTTTGTCTTCATCATAATAACGATTTTTATATTCACTTTCAATTTCTTCATTGTATTGGTTTATTCATTTTAAAAAGCGATCTTGATATCATTTTTGTCTAGATGTTTGATTTTCAAAAAAAGGTCAACCGTTTCAGTGCGAATTTTCAAATAAATTTTTAAATTGAGATAATGTAGAATCATTTTTTATGTATTTAGATCAGTCATTTAAAATTGAATTTATTTCATCATTTATTTTCTTCAAATAGGTTACATTTTTATAAAAATTAAAATTAGTATTATCACTAGGAGTAGATGTAAAGTCTTCAAAATATTTTTTGAATTTAGTACTTTCATCAACATTATTAACCATAAATTTATATGCTTTTTCTTCAAAAGCTTTTGAGTTTTTTAATAAAAAGTTTTCAAATGTGAAATCAGTTATTTCTTTAGTTTTTTCATATTCCTTATAATAAAATGATTGATATGACAATTTGTGGTTATCCTTGTCCATAAATTTAACTAATTTATTAAAGTTATTTGTATAAATAGAGACATTTTGTTTTAGATTGCTAACAAAATTATTTTTTTCATATTCACCCATGTCTCAAAAACTACTTTGACATGCAGCTGATGTCAAAGGAGTGGTTGTGGTTAAAACTGTAAAAGAAATTAAAAATTTTAATTTTCTTCTTTTTGACATAATTTCTTTTCTTGCAATGAAATTAAAACTTTAAGATCTTTTATTTCTTTAAGAGTTAAAGTTCGATATGAGCCTTCAGGCATTTTTTCAACTGTTAAGTTAGCAAATGACACTCTTTTAAGATTAATAACTTCATGATTTAATGCTTTGAATAATTCTTTGATGTGATGGTATGAGCCGACATGAAGAGTTACTAAATAACTTTTAGTATCAACTTGGTCAACTATTTGTTTTGAAAACTTACCATTAACAATCAAACCAGCGTTAATTAATTTGAATTCTTTTAAGCTTAAAGGAGAATCAATTCTAACACGATATGTACGTAAAATTTCGTATTTAGGATGTGTTAGTTTATTAATCATTTCTCAATCATTTGTTAATAACAAAGCGCCAGTTGTATCATAGTCTAAACGACCTACAGGAGCAATTCTTTCTGAGTAATTAATTAAATCAACAACTGTTTTTCTGCCCTTAGGATCTTTAACTGTTGAAATTGTCTTTTTAGGCTTATTTAATAGGAAATATACAAAATTTGGTTTTTGTAATGAGATTAGTTGATTGTTAATTTTTATCTCATCGTTTTCACTTGCTTTGTCACCTAATTGAACAATTTTTCCATTAACTTTAACTAGTTTTTTTTCAATATATTCTTCAGCTTTTCTTCTTGAACAATATCCTGTTTGTGCGATTAGTTTTTGAACCCTAATCAATTCTTTCATTGCAAACCTTTCTACCAAAAAATTTATTTCATTCATTTGTCATAAATTTTTTGTTGTTCTTGTGTAAATATTATACCTTTTTCTTGGTTATATTCATAATTGCGTTTTACAACTTCTTTAAATCCTTCATCGAAGTTACTTAATGCTAGTTGTCTAAGCTTTTGAACTCCGCTAAAAGCTCGACCATCACAGATTTTATCTGAGATATATAAAATTTTATCAAGAGATCCTAATTCTAGATCTAAAGTTGTATGAACTTTTATTGCTTTGACTATATCTTCATTATTTATTTGATAGATTTCTTTAACTCATAAAGCTCCACAAACTTGATGTAAAACATGATTTGGATAAATAGATTTGTTGTTAGCTTTTTTATCATATTGAGCAATAAATTTTCTACTTTCATTTTCACTTAATTCTTTACAAATATCATGAAATAGTCCAGCATAGTAAGCCACTTTTGCATCATATTTTATTGTTTTAGCTAACTCAGCAGCAAAAGTTGCTGCAAAAACTGAATGTTTTGCTCTTTTTGCACTAAGCAAAGCGTGAACTATTTCAAAAGCATATAAAAAGTGACTTCCTATATATTGGTTAACTTTTTCAGGTACCATTCAAAGTTGACCTTTTCTAACTTCAGTCGAACTTTCTTCAAAAAGATCATTGTTTAAAATTTTAACATTGAATTTTTTAGCATTAATTTTATTAAAATTTTTACTTCTTTTAAATACAACGAATTGAGCTGTTTTTGTTATTTCTTCGATATATTCTCATTTATTTAATTTTGGCAATAAATCGCTGCCTATTAAGAAATATAATTCATCATTTGGATATTGTTGTTTGAAATATCTAATTGTTTCAAAAGTATAACTAACGCCACCTTTTTTGATTTCAAAATCATGCACAGCATAATTGCCTTCTAGATTTTCAATAGCTATATTAATCATATTAACTCTGTCTTTATTAGGGGCTACTTTATTATTCTTTTTAAATGGGCTTATCGCTGTTGGTATAAAAAACATTTTGTCTAATTTAAGTGTTTTATAGGCATATTCAGCAATTTTGATATGTCCACTATGGATTGGATTGAATGATCCACCAAATAATCCTATTTTCATAATTCTCCTTAATTTTTATATTAAATTATAAATTCTTTTTTGTAATATCCTCTTGTAAATATCTCATTTGTATATTTTGTTTTTTCTTTTCTTTACTATATTCTGAAGCCAATTTTAATTTTTTGTCACGCAATTGATAATTGACATCGTTAATAATATTTTTAATTTTAATAGTTTGAGAGCTTTCATCTTCAGGAGTTGTAAAAATACTTAGTTGTTTGCCATAATCAAATTCATTTACTAATTTATGTAATCTAACGCCAATACCTCTAATAGGTTCTTCATTTCACAATATTTCAAAAAGTTTACTTGCTTCTTTAAAAATAATATCTTCATCATTAGAAACAACTGACATTGTTTTTTGTTTAGAATGTCAAGTTTTATTTGTGTCTCTTATTGAACATGAAATGACTTCTGGTAAGGTATTTCTATTTTTTGCTCTTTTTGAAACTTTAACACATAAACTTCTTAGAATGAGCAATATTTCCTTGCGATCATCAATATCATATGTTAAAAAAGTAAGTTCATTACCTATATTTTTTAAATCATTATGTTCATAATTTAATTGGTCATTGCCATTGCCTTGAGCTTCAAGAATTATGCCTTTAGTTTTAACACCAAAAATTTTTTGTATTTTAAGATTTTCTCAATTACATTTTGCTAATTTGCCAATAGTATCAATATGCATTCTAATTAATCTCTCAGCTGTTGGAGCACCAATACCGAAAAATTCAATAATTGGTAAAGGCCAAATCCTTTTTTCTATGTCTTCCTCTTTTGTTTCTAGAATTCCAAATGGTTTACCTAAATTAGTTGATAATTTTGCTAAAAATTTGTTGTATGAAATTCCAATTGTTGCAGGAATGTCAAAAGTATTTAATATGTCACTTTGGATACTTTTAGCCAAATCTTTTGGATCTCTATTTTTGATAATATCAGTTACATCAAGCCAACATTCATCAATTGAAAACACTTCGATATTTTTTGTATATTTTTCACTAATAAATTCAAAAATTTTATTTGATAAAGATAAATATAAATCAAAATTAGGTTCAATAAAAACTGTGAGAGGATCTATTTCTAAAATTTTATTTTTAGGTCAGCCAACCTTAGCACCTTTTCTTTTTAACTCATAGCTAACTGAAGAAACAATTGACCTTTTCAGTCTTCTACCAATAGCTACTGATTTGCCTTTTAATTCAGGATTAATTGAGCGATGAGCACTTACAAAATATGAATCAAAATCCATATGAAAAATTGTTTTTTTATTTTCCATTTTGAGATTTTTCCTTTATTGCTCTAGCTAACATTAAAGCACAATTAACACGATTAAATTGTGTTTTGACATTATCAAAAATTGCTAAAGTTCCTAGCTTTTCAATATCATTTTCATTTAATTTTTCACCTAGTATAAATTTTTCGTAAAGTTCTATCAAACAGATACTTTCGTCAATTTTTTTGCCCTTTAATAAGTCGATTAATAAATCAGTTGAAGCTATAAAAAATGCACAACCTTGACCATCAAATTTAATGTCTTCAATTTGATTATTTTTTATATTTAGTTCACATTCTAAATAATCAGCACAGCTTTCTCCGTGCTTAATAATTTTGTTTTTTAAACTTGAACTTTTGTGTCTAGGTTTCAAATAATGTTCCATTATTATTTGACGTTTTTGATCTTTATTATAAGACAAGGAAATCACCTCCTTCTTTTAATGCTTTAACTAATTTATCAATATCTTTTTTGTTGTTATAAATACCCAAAGAAACTCTTACAAAAGACTTTTCTTTGGTGATTTTTTTAAGATAATGATCACAAAATACACCTGCTCTTACATAAATATTTTTTGTTCCTAAATAATGTGAAACATCATGACTATTATAGTTTTTTATATTGAATATTATAATGTGATTTTTAGGATTGCTATATAGTTTTATGCCTTCAATTTTGCTTAATTCTTGATAAGCATAAAAGCTTAATTCGTCTAAAATTTTTTGAGTTTCACAATAACCAATTTGATTAAAAAAGTTTATTGAAACACTAAACATATTAATTGCTGCTAAATTAGGTGTTCCAGCTTCAAAGCGATCAGCTGCTGTTGACAAAGTATATTGTTTTTCACTGTCAATATTGTTAATTATTCCACCGCCAACAAAAGGTGCTTCAAGTTTTTGTAGAATTTCTTTTTTAACTGCTAAAACTCCAAAACCGGTAGGTCCATAAAATTTATTTGAGCTAAAAGCAATTGCATCACAATTATTTAATGAAACACGTTCATAAACTATTGCTTGAGCCGCATCATTAACTACTAAAGTATTAAATTTTTTAGCCTTTTTGTAAATAGCATTTAAATCAATATTGTTTTCAAAACTATTTGAAACTTGACTTACTGCAACAATTTTAACTTTTGAATTTAAGTCATCTAAAACATTTTGACTTATTTTAACACTAGCCCCAATTTCATTAGCTATTTTAATTCAAGGCGCCATATTTGAAGAATGATTATAAACATCTAAAAGGATAATATCCCCTTTTTTAATTTGATTTTTGATCATTAAAGCAAACTTGTTTAGTGAATCAGTTGCTCCTGAAGTAAATATAACTTCTTGTTCTTGACAAGACAACAAAGTAGCAACTTTTTTACGTAAGTTGTTTATTTTTTGCGCGCTTTCAATTCCTAATTTAGTGTTAATAGTACGGGTACTAATTGACTCATTTAAATAAAAATTGTTAACTGCATCAATAGCTATTTTTGGTTTTAAAACTAATGCACTACTATCCAAATAAGTTATCTTTTTTGCTAAAGGGAATTGTTCTTGAATTAATTTGTCTAATGTTTTCATATTTTAAATTATAAAGTTATATTTAATATTTGTATACAAATGTTACATATAAAAAATAACGTTTTTATAAAAAATATATAATTAGTACAACAACTTAAAAAAGGGAAAAATGAAAGAAGTTAAGAATTAAAATAGAGCAATATCTTACTAATGAAAAAGAATTGCTCTTATTTTTTATAATGTTCAATAATTTTTTTGTATTTTTCTATAACTATTTCAATTGCTTCTTCTCATGAATTAAATAAATTGTTCATATTATTTTTCACATTAATTAGATTATTTTTATCTTTAAAAATTTGTTTAATTTTTTCGTACATATTGTCAACATCGTCTTGGCAAATAAAACTATTTTGTCCATCTATCACATTTTCGGCACATGCTGTGTTTTCAACAACTAAGAGAGGCAACTTTAAAGCTGCAGATTCTCTAACAACAAGTCCATAAGTTTCATATAAACTTGGATAGAAAAAAAGATCATGAGATAAATAAATGCCTTTTAATTCTTCTTGATTTTCTATAGGGCCAGTAAACAATATTTTATCTTTTAAACCTATTTTATAGGCATATTTAACAATTTCCTTGTAATTGATATCACCACCAACCATTGTTAAATGGTAATTAGAATTTTCTTCAACAAGTTTTTTATAAGTTAATAGAACTTTTTTAATATTTTTTTCCCAAACCAATCTTGAAACATATAATAAATTTTTATTATTAGTGTTTATATTTCATTTTTTTATAACTTTATTTTTTAATTCATCTGCATTTTCTGGATAAAAGAAATTTGTCCCTAATTGTACAATTTCACTTTCTTTTTTATATCCATATTCAATTAAAGCTTTTTGCGCAACTTTTGACACAGGTCAAATGTAATCAAACTTATTAAGTTTTTTAATCATTAAATGTGTTAAAAATTTTGCAATTGGCTTAGACTTTGTAAAACGCAAAAATGAATCATAAAACAAAGTATGAAAGCTACAAATAGAGGGAATTTTAAATTTTTTTGCAATTTTATTTGCGATATCTCCGGCATAAAAAGGTGTATGAGAATGGACAATATTAGGTTTGAAATTTTCGATTTCTTTTCAAATTTTTCTACTCAAAATAGTGTTCATTACTTCATAACCACCAATTTTAGCTAATTGTCTAGGTATTCTAATGACAGAATAATTAAGACCTTTATCTTCATCTAATTTCATTTTTTTGTATTCTGGAGCCAAAACCAGAACTTTAACATTTTTTTTAATAAAATTAGAAGCATAATTTTGCATTACCCTGACAACTCCATCTATTACAGGAGTAAAACTATCATTAATTAAAATAACTCTAATTTCACTATTTGTCATTTTTCTTTGCCCTTTCTTTTTTACAAAAAACTTTCATATCATACATTCCTAAATGTTCAGACATTTTGTATTTTTTTGGAATTCTTCAATTTGGACCATAGTTTTGTAAAAATATTGTTTCATAATTTTTTGGCACCCGAATTTTTATATCTTCAAATTTAACATCAATAGTATCAAAAAAATTGATATTTTCATATATGTTAATTTTTGTAGCCTTTTTAAAAGGCCAATTAATTACATGATGAATATTTGAATTTGGTTCATACAAAGTCTCAAGAGCTTTTTGATAATTTGGTTTTTTAAACATTCTTGTTCAAAATAATAAAACTTTAGCAATTTTCATTCCTCATTGAATATCAAAAAACTTTCTTTGAGAATAAGCGTTCAAATAACCCAATTTATTTTTAAGAGATAAATATTTTTTTATTCTTTTTTTAGTTGTTGGTATATCGATAAAAAGATCTAAAAAAGTTGCATCTTTTTGAGAATCTTTATCTTTTGAAAATTTAGGGAACATTAAAAAGCTGTTTCTTGTTTCTCAATCTACAATTAAATTTCTGTAATTTTCCTTTAAAAATTTATATGTTTCAATTGAAATTAATAAATCAATATCATCATCTCAAGGTATAAATCCATTGTGTCTTATTGCTCCTAATGCTGTTCCATACATAGCACAATAATCTAAATTGTGTTTATTACAAATTTCAATAAACTCTTTCAACAGTTCATAAACTTTAGTTTGTTTTTCATTCATAGTTTTTATTATACTTTAAAAATTTTTTGGCAATAATAAAAACTATTATCTTGAAGATAATAGCCTTATTAATTATTTAAATTGCATTTTTGCATTGATCAGTTTCGATATATTCTTTTAGATTGTCATAAGTGTATTCAACCATGTTTTTAACAGCTTCATCAGTATAAGAACCAATGTGAGGTGTTAAAACAAAACGAGGATAGAATTCTAATAATTGGCGAAATGTTTTGTTTTCTATTTCTGCATGCTTTTTAAAGAAAATTGATTTTTCATTGTTCAAAACATCTGTAGCAATAGCTTGAATTTTGCCACTTTTTAGACCTTTTAATAATGCTTCTTCATCTTGAATTTGGCCTCTAGCTGTGTTAACAATAATTACACCTTTTTTCATTTGCTTAATTAATGCATCATTAATCATTTTGTCGTTTTTACCTTTAATATAGGGGCAATGGAAAGAAATGATATCTGATTTTTTAACTAATTCTTCTAGAGTTTTTGTATATTTAACAACTTTTTTGCCTTCTTCTGAAGGATATAAATCGAAGCCTAAAACATTGTTTGTCATAACTTTAAACATTTTAGCAGCTGCAAGTCCTATTTTTCCAACCCCAACTATTCCAACTGTTTTATTTTTTAATTCAGTCGCAAAACCTTCAGGATCAATAGTAAAGTCTTTGTTAAAACCTTTATGTCCAAAATGAAGTGTTTGTCTTGATAACATTAAAGCATGAGCAAAAGCTAATTCAGCAATTGCTGTTGGTGAATATGAAGGAACTCTAGCCATTTTAAAGCCTAATTTATGAGCATATTCTAAATCAATATGGTCTGTTCCGACAGTTCTTGTTAAAAGATATTTAATGCCATTTGCTTTTAATGCATCTAACATTTCTTTATTTATTGAACCATCAACTCTAACAATAACAGCGTCAAAACCTTTTGTTAAATTGACATTTTCCATAGTTAAACTTTCACTATGAATTTCAAGTTTATAACCAAATTTTTTGTTAATTTCTTCAAATATTGGTTTTTCAACATTTCTAACTTTAAAAGTAATAATTTTCATAATTTTTCCTACATAAATACTAATTCTGCAACTACAATTCAAATAGGTAAAAAGATAACTGCAAGTAATGTACTAAATGCTGAAGCAGAGCTTGCTAATCTAGCACATTTATTTTCACTAATTGCAAAACCAACAGCAACTGTAGCAGGTGGTGTAGCTGCAAAAATAACCATTGCAATTCCTACTTCTTTTTTAACTAATCCACCTTTGTTTAAACCTCAGAAGATTAAGAAGATTGCAAGTGGAATAACAATCAATTTAATTAATGAATATATTCAAACTCATTTATCTCCTGCCGCTTCTTTTAATTTACTTTCAGCTAGTGTCATACCAATAGCTAATCAAATAAGTGGTGAACATAAACTACCTAAAATATCAATAAATTTGTAAATATATGGTAATGTTGTTTTTCATGTAAATCAACCAACTGGTTTAGTGGTTTTAATATATTCATTATTTGCATTTAACATATAGAATACTTTTTTGTCTGGTGTTAAAACTGTTTTAAAGTTTTCACCAAAAGTAGCTGAATATTTAATAACTACATCACTTCCATTAACTTTAATAGTTTGACTAAAATCTTTATATGTTGCTCCCGCACCTGGTATTAATTGAGTCAATCAAAGAATTAATCCAGCAAAAGTTGCAATAATAATTGGATTTAAAAACATTGATTTTGCTGAAGCTTTAATATTTTCTTTGTTGAATTTTAAACCTTTCATTTGCATGAAGCAGAAAGAATATAAGAAAATTCTATAAGGAACATTTCAAATATTTGCTGCTAGAACACCATTATTATTTGAATATAGTTCCTTTATAATAGGCATACCAAAGAATGTTGTTGATCCGAAGATTAATAACATTCAAACAACCATTGCACGATGACGAGATTCATCAACTGTTTTTGTTTCACTCATTTCACCAATAATAGTGTTTTCTTGAGCATTTTCCACTGATTCTGGTGTTTTATGAGGCATATATTTAGTTCATAATCAAGCCACTAATGAAAGCAAGATATAGAATCCAAATGCAATTCCTAAGATAATACCTTGTTCTTGAAGAAGTCCAAGACTAATATCGCTCATAAAGCCTTTGAGTGCTAAAGCTGGTAAAGCAACTTTCATAACTACGCCAACAAGTGCAGCTTTTCAAGTTGATTTAAATCATTTAACTTTAACCAACAAATAACCTAGTCCGATTATTACAATAGAAGCAATAATTGCGCCTCAAAGATTTACATTAGTTAAAGTTGAAACTAATTTCTTTGTAACATCCATTTTTCTCCTTTAATAATTTGCTCATAAATATTATATTTTTTATATTATAAAAAATGAAATAAATAAAATGGTTTTGTGGAAATTTTCTAGAAATTGGAATTTTTACAATTATGAAAATTACTATAAAACAATGATTTTAGCAAAAAGACTAATAAAAAAACTTTACACTTTACTAAATAAATTTAGCAGTATAAAGTATTTGAATTAATTATTTTGTTCTTGGTTTTGATGACTATTATTATCATTTGTTTCTTCTACTTTTAGTTCTTTCTTTTTCAATTTCCTAAAGATAAATTTATCATCAGGTATTAAGGTTATTATTTGACTTATTAGAATTAAAGAAGCTAATAAATAGCAATATCAATCATAAAATTCAAGTCTCATCAAATCAAACAATATTGTTCAACCAACATAGGAAATATTTAATCCTGTAGCCATTGGAACACCTATTTTATCAAGTGCATAATAGAATGTCATATATGAAATGCAACCCACAATTCCAGAAGCAACAACTAATAAAATGTTTGCTTTAGCAAATAATTCACCCATTTTTCCATAGATTTTTGCTGACGGTAAAACAATGCTACCTAAAACAATCAATGAAACAACATATCTAAAGAATATAGAAACAAAAGGATCAATAGCATTTTTCATTGATAAGGTTGATAAAAATGCTTCCAAAGCTCAACAAAGTGCAGCTAATATTGCAAACAAGAAACCTCATCCATTTTTAATATCGTCTTTATTAAATTGTAAAATTCCTAAAGTTAAAATACATAAAAGTGAAATTATTGATCCGAATAAACCATTTTTTGTACTTTTTTGTTTCAAAAAGATAAAAGCAAGCATTGCGCCAACAATAGGATAACAAACTGTAATTGCACTACTTATTCCAGTACCGACATAAACAATACCAAGGACATACATTGTCATACCCATTGGTGCCCCAAAAAGAGAGCTGAGTAATAATATTCATATTTTTTTATTTTTGAAAGCTGCAACAAATTCTTTATGTTTTTTAAATGAAAAAATAATTATTATGCTAACTAAAAAGCCAAATGTATCATTTAAAAAAGTTATAGCTAAAGCTAATTTAGCTGGGCCTCATTCTCCTTGATTAAAAAATGAACCGGAAGGAATTAAATTTGTAAAAACAGAAATTAAAATTCCATTTAATGCTCACATAATCCCTGAAAATAAAGCAACAAATTTATTAAATAAATTTTTATTTTTAACTTTTTTATCGTTTTGAATTTCATTAACTTCTACATTTGAATTTTTATCATTCATATTTCACCTTGTTTACAAATTTTAATGTTGCAGAATAGTAAATTTTGTAATAATCTCTGAAGAATTCATTAAATATTTCTTTATATTCGCACCAATATGTTCATAAGAAACCTATTGAAGCAATTAAAATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGACATAAAACTTTTGGATAATTTTTACTTTTATGATAAGCAAGCATTTTTCTAATTTTAGCTTCATTGTCTAATAAATGTTTTGGAAATGTTTTGTATTTTTCTCCAATTATTGAAATGTAATAGTCCAAAATTTTTAGAATATCAAATTCAAAATCAACTGATAAATTTTGATTATGTAGTTTGCGATAACTATTTAAAGCTAATTCCATTTCTTTATAATTGTGTCCATCAATTGTTTTTGAGTTATTAATTCATTCAGTTATTTTAAAACCATGTAAAGGATCATCAATATTATTTTTGGTTTCAAAATAAATTAAATTTTCAACTAATTCATTGCCTTTTAGTTTTTGATAAATTTTTTCTTCTTGGTATCTATTAATTAAACTCTTTGAGCCATCTTTTGGTATTCTAGCAATATATTTTTTATCTTTTATTTTAAAAGAAAATGAGTTATTAGTCATTCCTTTTTTAACTGGTTGAATATCTAAAATATCTTCAAATTTGGCATTAAAAACTTCTTTAATTTTGTTTAAATAAACATCGTTTATAAAGCAAGGAGCTTTTCTATTAAATGTCAATAAATCTTTGAAATCGTCTATTTCATAGACCTTTTGGTCACAATTGTATTTCTTAAGCTCTATATCTTTTAAGATATCCATCAAAGCAAATTCTCAATAATCATTTTGCTTTGAAATTGGATTTTCAACTCTCTTAATAATTGCATTTTTAAATTTCAAGAAATCTTTTTCATGAATAAATGAAGCACCTGTAATAAATTCAAAATTATCACTTTCATAGATTTTATTTAAGTCTCTAATTCTAAAGTCAACTATGTTTTTATTCTTTTCAATAATTTCTCATTCAGCTTTATTTGTTGTACTACTCAAAGTATTAACCCATGAGTAATTAAATTCCTTATTAAAGATATCATCTAAAAATAAAGTGTCATTGGTTGTAATTAATAAACCACCCTTAATTGTTTTCAAATCTTTAATTGCTAGATATAAACTAAATAATGAATTAGTATCTGTGAATTCTTTGTTGAATATCTTTTTGATATTTTCTTTTTTAATTAAGTCATTTCAAGATTGATAATTGTAGCCAGTTACTATTGTCTTATTTTTATAATCTTTTAAATAAGATAAAAGATGAGGAACAATTTTTAAATCTTTTTTAATTTCTAATAATCCTTTTGGCGTCTTAAGTGAAAGAGGCGTTAGTCTTGAACCAAAGCCAGCAGCTAAAATTATTATTTGAGTATTTTTGTCCATTTTTCTCCTAATTTTGTTATAAATTATATAATCAATGATATTATAACCATATATAAAAACTATAAATTAAGCAAATGTTCAAAGATGCTGAAAAAAATTTTGTATTTTATTAAATATTTGTTTCTCAAAAAATAAATTTAATATATAATAAAAAAGCAATTTATAAATTGAATTAACGTTTAAGGAGGAAGTCATGAGACAAACCACTATTGTAAACAAAGAAAAAGCTGACAAGAAGTGATATGTTGTTGATGCTGAAGGACAAGTTTTAGGTCGTTTAGCCGCTTTTGTAGCTTCTGTTTTAAGAGGTAAAACAAAACCTACATACACACCAAATGCAGATATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAATATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAATTTCACTTTTTTATAATAATATTAAGTTTTGCTCAAATTTTGAGTGAAACTAAAATGCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCATTTCATTAAATTGACCAAAACCCTTGTTTTATAGGGTTTTTTATTTTTTATTTTTTCTCAAAATTTTAATTGCTATATAATATAAAATTAATATGATGATTGATTTATGATGTCGAAATTTTGAGAATCAATTGTTTTAAAAAGGAAAAAAATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGATACTCACTTGCTTATGGTACTCTTTTAGGAGCTACAAGAAATAAAAAACAAATTGCATGAGATACTGATATTGATGTTCTAATTACAGAAGAAACATATGAATTCTTAAAAAAACATTATCCACAATATATTTTGGATAATACAACATCGCATCACTTTCTTAATTTTCCACGTTTTGTTGATAAGATTGATCAAAATGATCCTAACAATATTTATATCGATATGTTCATTATTGTTCCTTCAACATGGAAGAAAGCTAAAAAAATTAATAACTTTTGATACAAATTAAGATCAATGAGAAGAGCTTTAGCTCCAAGAAAATGATTTATTAATATTTTTGGAAATATTTTAATGTTTATTTTTGGCTGAATGTTTTTATTTTGAGTACCACCTTTAGATATTAAAACAACTATTGAAAAAACTAAGGTTTCAGAAAAAGAAAAAGAAATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGATATGGAAAGACATGAAGAATACCAATTCAAACTAAACATCCTATTTATTATGGTTATTATGAATTTAAAAAAGTTCCAAAAAAATATCGAGATTATTACAAAAATGAAAATGCAGGCCAATAACCTGCTTTTTTAATAAAAAAAACTCAAATTAATGAGTCCTTTTTTGGTCTAAAGTGATATGGTGCCCAAGACAGGACTTGAACCTGCACGGATCTCTCCAGTGGATTTTGAGTCCACAGCGTCTACCATTCCACCACTTGGGCAATTATAAATTATTATAAATGAAGTTTTATTTTTAGTAAATAATTTTTCTAATAAAGCAAAAAAAGTATAAAAAAATAGCAGCTCCCTATTTTCCCATCTCTGGTATCGTCGGCACTAAAGGGCTTAACTACTGAGTTCGGAATGGAATCAGGTGGGTCCCCTTCGCTATAACCACTGGATATATTATAACATGCAATTTAAAAAATCAAGCAAAAAAATAAAAAATTTTTTTCAATTTTTTGAAGTAATTTTTTTAGTCAAAAAACTGAGTAAAAAATATCTTTGTTTAATATTAATTTTTATATAATTGTAATGTATTTATTAAGGAAGAGATGAGAAAAAATAATAAACAAAATAATAAAGAAAAAGAAAGCAAATTTAAAAAATTTTGAGAAGAATGAACAATCTTTCCAAAGTGAACTATTAGAAAAATTTCTTTTGTTGGAATCTTAATTGCAATTTCGGTTGTATTTTTAGTTATTGCTTCTTCTCTTGTTCCTATTGCTTCAATTCCAACTTATAAAATAAGTTTTATAGGTTTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGAAAATTAAGCAAAAAACTTGATAAACTAAAATTAAAAAATACTAATGATAAAAAAATACTTAAACTAGAAAACAAAATTATTCTAATTCAAGAAAAAAGAAAAAGTATTCGGATCAATGGAACACAAAATATTTTGCTAAATATTAATTTATTTGTTGCTGTTATTCTTTTAGCTTTAATATCATTATTTATTGTCTGATTAGTTGGCTTTGTAATTCCTGAAAGCATTATTAAAAAAGGTGTTATTTCGAATAGATGAGGGCTTTTAGCAACAATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGATTTAACATGTTTATTATTTATTTTACATACAATATTATTGCTCCTCTTATTTATAAAAATAAAAGTATATCTTATGATAGTTAATAAGTGAACCTCAAATTTCAAACTTTTTGGAAAGGGGTTCATTTTTACGCAATTAAAATTTAAAAAAATAAAAAGGAAGAAAATCTAAAATAAATTTTGATGCTCAAAAGGCAACAAATTTTAAAATTAATATTGAAAAAGTAAATTTTTTATGAGATATTAAAATCACAAAATAAACCTGACAAAGATGAAAATTTGAAAAAGATTATATTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCATTAAGAAATAAAGGAACAATGATTAATCATAAAAAGGTTTAAAAATTAATAAAATCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATCCAAAAGGATGTTCACAAAAAACATTCCAAACTTGTTTTTAGATAAAGAAAATATCGCAAAATATTTCTTAAGATACAAAATTTTTTAGTAATAAATATTTGAAAATACTAGGAACAGATGTTATTGAATTCAAATTAAAAAAATGATAAAAAAGCATATTTTCTTCTGTGGTTGATTTTGAAAATAGTGAGATTTTAGGTTATTCGATTTCTAAATCACCTAACTTAAGAATGGGGGGTAAAATGTTGGAAAACGTAGAAGGGAATGAGCATAGCTTAAACAATGTATTATTACATTTTGATCAAGGATGACAATATACTTATGAAGATTATATAAATTATTTGAAAAAGAAACAAACAATTCAAAGCTTGTTAAGAAAAGGAATCGTTTAGACAATAGTCCCGCTGAATGTTTATTTAGTGTTGCAAAAAGAGAATTTTAATTTTGAGAAAAAATAGATTTAAAAGTTTTAAAGAACTTAAAACTACTTTAGATTATAATAATGACAGAATTGCTAATAAATTAAAAGGACTTAGCCATATTCAATACATGAATAAGTCCAAACATAATTAAATAGTTCAATAATTGAGGTTCAAACTATAATGATTGTCTGTGCAATTATTTTTTTATATGTTGATTTTTTATATGCTTTTTAGAAAAAGTCACAAAAAATATTAAATTCTAAAAACATTACCATTAAATATTATTTATATAAATAAATTAATATTAATTTATAATATTTTAAAATATAATTACTTTATAAGTTTTAAAGGAGTCTGGAGGTTGTATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGAGTAGCAGAAGCTGAAAATAAAAATTTAGATTTCAAAGATCTAGAAACAAATAAAAAAGGCGAATTATATCGTTTATTAACAGTTGATTCACGTTTCGTTCATAAAGGAAATAATGAATGAACAATTAGAAATGGTTTTGAAATTAATAAATAATATTTTGAAAATAGGAGTACAAAATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGAGCTAAAGCAGTTTTATTAACAGCTCAAGAAGAAAATTCACCTGTTATTGTTGCAACAAGTGAAGGCGCAGTTAAATACATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGAAAATCATTAAACTTCGACAAATTAGTCGAAATTAGAGATGCAGCTAAAATTGGTATAGTTTTACATGGCGGTTCTGGTATTCCGGCTGATCAAATTGCTAAAGCAATTAGTTTAGGTGTTACAAAAATTAATGTTAACACTGAATTACAACAAGCAAATCACAAAGCTTTAAGAGCTTATATTGAAGCAGGAGAAGATCTTAAAGGCAAAAACTTTGACCCACGTAAACTTTATGCACCAGGTTATAAAGCTATGTGTGAAACAGTTAGAGCAAAAATTAGAGAATTTGGTTCAAATAACAAAGCTTAGTTAAAATTTAAGAGGAGTTAAAAGCCTCTTTTTTATTTTTCCATTTTTTTAAATTTATTTATAAAAATGACTAGGTATAAATATGAAGAGATATTTGAAACTTTTTTTAAATTTTTTGCCTTTTACTTGTTTATCTGTTGCTGGTTGTCGACAACAGATTATTAATCCACAATTAAATCAAGTTTTTGATGTCAAATGACATTATCAAGTAGTAGATGGTGACACGTTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGAATTTGCAAAGTTTATAGTGCAGAAGGTAATGATTTAGCTTTTGAATTAGTTAAACAAGGATTTGCTAGAGTATCTTATATTTCAATTGATTATCATGCTAAATATTATTGAGTTAATAATAAAGAACAAAAAGAATATTTTTGAAAATTAAACCAGTTGCAAAATGAAGCACAACAAAATAAAAGAGGATTTTGAAAAGAAAAAAATATTAATTTAGTTTTTAATAAGAATTAAAAAAATACGCAAAATGCGTATCTTCTTGTTTGCTTATTAAGCAACAAATTCAGTAATACTTTCGTTTTCAACTACTAAAAAGCGGTCGCCTTTTTTAACACCATATGGTTTAAGAGCATTAAGAGCTTTGCTTTTATCTTTTTTGATTTTTGCAAATAATTCGATTGAATCTAATGATACTCAAACACTAGAAGTAACACCAAATCCACCGATTAGGTTTTCATCATTTAAAATACCAACGATTAATGTATTTGGTCTAAATTTAGCAACTTCACTTAATAATTGACCTGTTCTTGAAAGAACAACAGCAAATCTATAGTCACCATAACGTGTTCTGTTTGCAACGTCATAAGCAATTCTTGAACGTTTTGAATTTGAAGAATTTTTTCTAATTTCTTCTAATTGAACATCATAGTAATTTTTGTCATAAAATTCACTTTCAGCACGTTTGTTAATTTTTGACATAACTGTAACTGCTTCTAAAGGATAGTTGCCTGATGCACTTTCACCTGAAAGCATTGTTGAGTCAGCACCCATTTCTGTTGCATAGTAAACGTCAGTAACTTCAGCACGTGTAGGTTGTGGTGAGTTTTCCATTGAGTCTAACATTTGAGTTGCAACAACAACTGGTTTACCCGCTTCACGACATTTTCTGATCATTATTTTTTCAAAATAAGGAACATCATAGTAAGGAATTTCAAGACCTAAGTCCCCACGAGCAACCATAACACCATCTGATGCTTGAATGATTTCATCAATGTTTTTGGTTCCTAAGTGGCTTTCAATTTTAGAAATGATTTTAACGTGTTTTCCACCATTGTCATCTAATAATTTACGTAATTCTTGGACATTTTTAGATGAGTTAACAAATGATGCTGCTACATAGTTAATTCCGTTTTTAATACCAAATTTAACATCATCAATGTCTTTTTGAGCTAAAAATGGAAGAGAAAAGTCAACACCTGGTAAATTAATACGTTTGTTAGTTTTTAATAAGTGCTTGTTAAGTGTTTTAACAACAACATAACCAGCTTTTCTATCAACTTTAGTTACAATTGTTGATAATTTACCATCGTCAAAGAGAACTTGGTTTCCAACAACTAAGTCTTGAGCCATGTCATAAGCAACAGTTAACTCTTTTTCTGTTCCTTCTACGGTTTCATATTTTTCTTTAGTTGTTCAAACTTTAATTTCTTTGTTTGCTGGAATTTCTTGTTTTCCATCTTTCATTTTTCCTAAACGAATTTCCGGACCCTTAGTATCTAACATAAGAGATATAGGAAGATGTAAATCTTTAGAAATTTTTTGTGCAAGAACAAATTTGTTTAATTGTTCTGCATGGTCTCCGTGGCTAAAGTTAGCACGAACACAGGTTGCACCTGCTTCAATTAATGAACGAAGCATTTTGTAGTTATCACTTGATGGACCAACTGTAATAACTAACTTACTTCTTTTGTCAGTAATGTTCATTTATTCTCCTTAAATTAAAATAAACTTGTATGTATATATTTGTAATACTAAATATTATTTTATATTAAATTTGCAAAATAAATAACATATTTTTTTATTTGAACCATGATTAATAGTAAATTTGTAGTTTCATTATTTTCTTATTCTTTTTTATTAATAAAAAAAGAGCAATTTGCTCTCGATTTATGATTTTTTAAAACCACCTAAAGTTATATGTCCAATATATTCAGGAATTGAAGAAGACTTATAATTATTATTTGAATTTTTAACTCTAATACCAATTGCAAAGGTAGCTTCGCCTTTTGCTGGATCAAATGAAACTAAACCTTCATTTTTATATTTTGTTTTGTCTTTATCATAATCTTCTGTTTGTTTTGTACAATAGAATAATTGGTAAAGGTGACTATTTTTAAAATCATCTTTAAATTTCAATTGAATTCTTTTGTAGTAACCTTTACTTGCGTTTTTGTATTCACCGAATTCATCTGTTGAATTATTTATTCATTCAAGTGAAGTTGAAGGTGTTTTAAAGTCAATATTTTTAATTTCATCGTTACCTAAAATAGATATTTCAACATGATTTTTCAATACTTTTTCAAGTTCAAAATAATTAATATCTATTTCTTGAACAATAGGATTTTTTATGTCAATCATTCTTGATTTTATTAAAGATGTTTTATCATTTTTGTCCTTAGGCATTTCCGCTTCCGGCACTATTTTATAGGCATAAAAGTGTAAATTTAATGATTTTTCAGAAGCTTTTGTAAATCACATTCCACGCTTTGTGTCTAATTTATATTCGGGGTTATTGCTTTTTTTATCTAAAGTTCTTCCAATGTCAAATCAACTTTCAATTGTTGAATTATTTTTTAAAATTTCTTCTTTATTTTTGTTTAAATCACTTGAAAGATCTTTTTGTGGTAATGCAGCAAGTAATTCATCAAGACTATTCTTTTCTTTCTTGTAAAAAGTTTGTCTTATTTTATCTCTAATATCCATTTTTCAAGATTTAGGATTACTAATTAATTTTGTGTTCGTTTCAACGACGTCTTTTTCAGTAACTTTTTTAAATCCATTTAAAACAAAAGTTTTTGATGTTCAGTTTTTTATTCTATCACTAATGTTTCTTGTTTTTTTATCTTTATCTTTTTCATTAGCTTTATCTTCTAATGAAACAGTCAAATAGAGTGTACCTGTAAAATCATTTGCATATGAATGGTAGAAAATATTGTAATCTTTATAAAATTTGTCAATTCTATCAAAACGATTATTTCTTAATGAAAATAATTGATTTCTCAATGCCCCTTGAGGAGAAAGATCATCATTTTTTAATCAACCTTTTTCAATATTTCCTTCAAGTTGAGTTCTTCAATAATTTTTTTCATATTCTTTTGTGTTTTTCAAAGTTGGTTCTTTGTTGCTATTGTATGTTTCAAAATAGTAATAATTTGAGTAAGTAAATTCACTTGCTAAGTGATTTGCAATATTAGTTTGGTATAATTTGTCTTTAGGTTTTGCAAGAGAAAAAGTAAAAGAGCTTAGCTGTCTTTCAATACTTTTATTGTCTTTATTAGTTTTAACTAAAGCATAAATAGCCGTACCAATTCCAACTGCAACAACACCTGTTGCTAATGTTCCTAATGTTAATGATAATTTACGATTCATTTTTTTCTCCTATTCAAATATAATTTTTAAATTATATATCAAATATTTGATATTAATGATTTTTACTCATTATAAAGTAATTTTTCTATGTGTTGAACTATATCATCAAGGTTAATATTTTTTTGGCTTGATACCAAGAACACTTGAGCTGATTTTTTAAAAATTTTTGAATTTAATTCATTATGTTTTTTAATTAAAGCCGATTTTTCTTTTTGATTTAATTTATCAATTTTTGTATAAACTATGTCATAATTTCAGTTTAATGAATTTAAAAATGTCAAGGTTTCTAAATCATTTTTAGTAAATCTTGTTCGACTATCTATAAGCAAAAAGATATGTTTTGGAGAAGGTTCATTTTTTAAAAAATCTTTAACCGCAAGATTCATTTTAATGATTTTTTCTTTAGATATTTCAGCATAACCATAACCAGGTAAGTCAACTATATATTTATCATTGTTATCTGCAAAATAATTAATTAATTGTGTTCTGCCAGGGTGCTTTGATACAAATGAAATATTTTGATTTGTTATAGCATTTAAAAGTGAACTTTTGCCAACATTTGAGCGACCTCAAAAACAAACTTGAAAGCTGTTTTTGCAATCAAGTCATTGTTTTTTATCTATTGCTGAAGTTATAAATTTTCACATTATTTTTTACCTAATTTTTGTTTATGTCTTAAAAGAGTTGAAGAATAGTCTATGTTTTCATAGTCTGGTACTATAAGTATAGTTTCAAGCTCAGAATTTAAGTGATGATTAGCAGCTGCTAGTACTAGCTCATAGTCATAATCTAAATTATTTCTGGCTGATCTAACTAGGAACTTTACATTTTTTTCTTTAGCAATTTGAGCTATCAAATCATTTTCATTTTTTACCACAGTGACTGTTTTATAGTCTTTTAAGGTTTTTTTGACTGATTGATATCTTTCTTCTAAATTACCTAAATTGTCTTTATCAGGGTTAATTGAAACTATTACTATAAGCTCATCAAAAAGCTTTAGTGCTTTTTCAATTACTGATATATGACCTTTGTGTATAGGATCAAAAGATCCTGGAAAAATTGCTTTTTTAATCATAATTATTTATACAAATTTATTGCATGTTTTGCAATTAATAATTCTTCATTTGTTCTAATTACATATACTTTGATTTCGCTATCTTTTGTAGAAATTAAAGCATAATCTTCAATTTTAGAATTATTCTTTTTGTCATCTAATTTAATCTTTGCAAAATGTAGTTTATCAACTACCATTTGACGCATTGAAGGAGTGTTTTCACCAACACCAGCAGTGAAAACAATAGCATCTAATTTGTTGCCAATTTTGTTTGCATAATTAGCGGTAAAATCAGCTATTTTTTGTGCATACAATTCAAGTGTGAAAATAGCACGTTTATTGCCTTCGTCTGCTGCTTTACATACATCACGCATATCGCTTGAAACACCTGAAACTCCTAAAAGCCCACTTTTCTTGTTAAGAGCTTCTGTGATTTGGTGAATATCTAAACCAAGTTGTTCATTAATAAATTCATGAATTGAAGGATCAATATCGCCTGAACGAGATCCCATCATAATTCCTGCTAAAGGTGTAAGTCCCATTGAAGTGTCAAATGATTTGCTATCTTTAATAGCACATAATGAAGCACCATTTCCAATATGTAAGTTAACTAAATTAACTTTTTCTTTTTTAACTAATTCTTGGATTTTGAGTGTAATAAATTCATGACTAATTCCATGAGCTCCATAACGACGAATTTTTAAATCTTCTGTTCATTGATATGGAATTGGATAAATTGCATTGACATCTGGAATTGTTGTGTGGAATGCTGTATCAAAGTCAACACTCATTTTGGCATGTGGCATAACTTTTTTAAATGCTTTCATGGCTTGAACTGCACCTGGATTGTGAAGTGGTGCATAAATTTTAGCTTTTTCAATTAAATCAATTTCTTTAGTATTCAATTTAACTGTTGTTGTAAAGTAAGGTCCACCATGAACAACTCTAAAGCCAATTAATTCGATTTCTTTTGGATTGTCAATTAAACCAATTTGTGACATTAATTCTAAAATGTTTTCAACAGCCACAGTGTGGTTAGGCATTGTTACATCACGGTTAAATTTTTGACCGTTATATTTGATTGTTATTTTACCTTCTAATAAGGTAATCCTCTCTGCAATACCACTTGCAATAATTTCTAAAGAAGCTTTATCAAAAAGTGATAACTTTATTGAACTACTTCCCGCATTAATAACTAATACTTTTTGCTTACTCATAATATTCTCCTATTTCTCTGCCTGCAATGCAGTGATTAAAATTGTATTAATTACATCTTTTACACTTGAACCTCTTGAAAGATCATTGACTGGTTTTTTAGTACCAACAACAATAGGTCCAATTGCCCCATAATTTCCTAATCTTTGAGCTAATTTATAGCCTATATTTCCAGCATCTAAATCAGGGAAAATTAAAGTATTTGCTGGTTCTTTATAGTAATCTAATTTATATTTAGCTTTTCTAACTTCTAAATCTAAAGCAGCATCTAATTGAATTTCTCCAATAGCTTTAGTACCTTGATAAATGGTATTGAATTCTTCAACAGCATTGTGTACTAATTCACTTTCAGGTGTTTTTGCTGAATAGTTAGTTGAGAAACTTAAGAAGGCTATTTTAGGTTCTATCCCCATGCTTTGAGCAAATTGAGCAGCATTAAGTCCAATTTCTACTAAAGCTTTTTGATCAGGCTTAACATTTACTGAAATGTCACTGAAGAAGTAAGTATTTTTTTCGTTATGTACTACCATAACTGAAGAAATAGTTTTTACTCCTTCTCTAGCGCCTATAGCTTTAAAAGCTGCTCTTAAGATATCTGCAGTTGCATAATTAAGTCCGCCAACTACTCCATCAACTTCACCTGTAGCTAAAAGCATCATAGCATAGAAAGGTCTTGTTTTTAGGTTTCTTTGAGCTTGTTCAATTGTTTCTTTGCCTTTTCTTAATTCAAAATATTTTTGAGCTAAATCTTTTTGTTTTTGTTTATCTTTATTAATATTTATAAACTTAGTATTGGTTTTTGACTTAACATCAGTTTCAACTAATAAAGTTAATTCAACATTAGGAAATTGTTCTAATTGTTTAATGGTATCTAGTGCTCTACTATCATCACCATCTATAAAAGCAATTCTTTTTTTAGTTTTCATTGCTTTAATTCTTTTTTCAATTAAAGATGTAAATTTCATGAATACCTCCTATAAAAAAATATTCTTATATTAATTTTATATATATTATTAAATTTAATAATTAAATAGTTACAATATGGCAATTTTTATCTTAAAAGGTAAATAAAAAGAACCTTAATTAAGGCCATAAACCAGGACAAAAGAATTAGACTTAAATCTCAAATATATTTAAGTCTTTTTGTACTAGTTTAAAGCATGAAATGAGGTTTTATAGGAAAACATTTAAATATTGAAAAATAGATAGAAATTTTTATATTTTTTAAAGAATATTTAGACTATAAAATTACAAAGACGGAATTTAAAAATGAATATTTTAGAATCAGTCAAAGACAAATATTTGATCGAGAAACAATTAGAAGTATCAAAAACAAATTTAAAAAATACAATTTGGGCATGAGTATAGAATCAAACACAGGAAAATCTCCTAAAAAAGGTAAAGGGTCTGGAAGACCTAGAAAAAAGGGATATTGAAGCTGAAAAAAAGAATAGAGAAGAAGCATTAAGAAAGATGACAAGAGTTCAACTTGAAATCTTAATAACTGTTATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCGATTAAAACAAAGAAAAAAAGATTAAATATGAATATGAAATTAGACAAGCTTTTAAAGATTGTAAAGGTAGATATGGAAGAGCAAAAAGAAAAAGAGAAAATAAGAATACAAATGTTAAATATGAAAATCTATCTGAAAGAGATTATGATGGAAAACATAATGATATATTTGCTACTGTTGTAACATACATTCCATCTCCAATTGATGTTTTAGAAAATCATGTTTATTTATCTGCAATTATTCATCATAGAATTAAAAAAATAGTTAGTTGAAATTTATCAAAAAACAATGATAATAATCTCGTTTTAGAACATTTAAAAACAACTGATTTTTCTTCAAATTTTATTATTCATTCAGATCATGGCTATCAATATTCATCAAATGATTATGTAAAATTCATATCATCAAAAAATGAAAAAATCTCAATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGATATAATAATGATAGATTTATGAAAAAATTAGATCTAAAAACACCCCAACAAATGTGGGATGTTTATAAGAACAATAAATTTATTGGAGTCTAATTTTTTTGTCCTAGTTTAGTGACTTTAGTTTTTTAAACCCAAAAAGTTTGTTGAAAGCCCAAATTTTATGGTTTTTAAATTTATAAAATTATAATCTGTGAATATGTATTATTGATTTAGCTAAATTAGTAAAAAGTGAAATAACTGTCATTGGACCAACGCCACCTGGAACAGGGGCTAAAGCACTTAAATAGTTTTCAGGTATTGTTTCAATATCAACATCACCATGAAGAATTTTTTTGCCATCTACTTCAATTCAGTTTGATCCAACATCAACTACAACAGCACCTTCTTTAATGTTTTCTTTTTTAAGAAGTTTGGCTTCACCAGCAGCAACTACAATGATATCAGCATTTTCAACACCTTTAATGCCTGTGTTTCTATTGTAGGTAGAAACATTGCAGCCTTCTTGTTTTAATAGAAATGCTGTAGGTTTTCCAACTAAACTTGAACGACCAATAACAGCAGCTTTTTTGTCAATCATTTTGATATTGTAGCTATTTAATAAATCTAAAATACCAATAGCAGTAGCTGGAACTAAGGTAAAGTCATCCGCTTGATTGTAGAAATTGAATGCATTTCTGGTACATAAACCATCAATATCCTTTTCAATGCGAACGCTATCTAAAATAACTTGTGTGGCAAATTCTTTAGGTAAAGGAAGTTGAATAACAATACCATCAGATTTTTCATTAATAATGTCTAATTTCTTAAGCAAATCTTTTTGTCTAATATCAGCTGGAAAATGATGATGAATTGCTTCTACTCCTAATTCTTCAGCCTTCTTTAATTTGTTTTTGATGTAGTGAGTAGATGCGGGATTATCGCCAACTTGAATAATTGATAAAATTGGTTTTCTGCCTAACTTTTCCTTTATATCCTTAAATTGTGCAATGAGTTCTTTAGTTCTTTTTTGAGCAAGAGTTTTTCCATCTAAGATTTTCATTTTAACTCCTCCTTGTATTTGTCTAATTCTTCTTGATATTTAGCTAGTTTTTGTTTTTCTTCTTCAACTTTAGATGTAGGTGCTTTAGCTATAAAGTTTTGATTACTTAAAATATTGTTTGAACGTTTAACTTCGCTTTCTAAATTAGCTATTTTCTCTTTTAATTTAGCAATGTAAGTGTTTTTTTGCTCGGCTGATTCTTTAATATAAACATTAATATTTCCATCAACAAATAAAGTGTCTTTATTTTCACTAATTTCACTGTTTGATAGTGATTTTAGTGAATTTTTAACAAACTCATTTTGTTTAATATTAGTGTCATAATAAAGCACTTCTTTTTTACTAATATTATTGTCTTCACGATATTTTCTAAGTATATTTACAATAGAAATTATTTGTTCAATTTGTTCAAGATTTTCAACTTTTTGATTCTTTAAGTTTGGAAATTCTTGAGCTAATAATTCTTTGTTAAATAGTACTTTATATAAGTGATCAGTAATAAAAGGTAAAAATGGATGAGCTATTAAAAGTAATGATTTTAGGTTATCTAAAGCAGCTTTTTTATTTGGCATTATTTTTAACAATTCAACATATCATGAAGAAAAATCATTGTAAATAAATTTAGACAATTCTGTACCAACAATTGTGAATTCATAATTTTGCATCGCCTTATCAATTCTCTTTTTAAGAGCAACTAATTTATTATGAATTCAAATGTCAGCAGCTGTTTTTTTATTTTCTTTATCTTCAGGTAATAATTGAATATAACGAGCAATATTTCATAATTTATTACATAATCCTCAAGCTGAAGTTATTTTTTCTTTTGAGTAACGAATATCTAAACCAGGGCTTGTGTTAGTAATCATAAATCATCTTAAAGCATCAGAACCATTTTCATTTATAACTTGAATTGGATCAATACCATTGTTTGATGATTTAGACATTTTTTTACCAAATTGGTCTCTAACAAGACCATGAATTAATACTCTATCAAAAGGTTTTTGTTTCATGAATTCTAATCCAAAAAAATACATTCTAGCAACTCAAAAAAAGATAATGTCATAACCAGTTACTAAAAGTGAGGTTGGATAATATCTTTTTAAAAGAGTGGTTTTCTTAGGCCAACCTAAAAATGAGAATGGAGCTATACCACTACTAAATCAAGTATCAAGAACATCTTCATCTTGAACTCAACCTTCGCCGGGTGATTTAATTTGTACTTTAACTTTGTCATCTTTATATCAAGCAGGAATTCTATGACCTCATCAAAGTTGTCTACTTATTGTTCAGTCATGAATGTTTTCCATTCATTTTTTGATAACATTAACAAATCTTTTTGGATAAAAGTTAACTGCATCTTTTGATTTTAAGTGGTCTAAAATATCTTGAGCTAATTTGTCCATTTTAACAAATCATTGTGGCATAACAAGAGTTTCAACTGGAGTATTTGAACGTTCTGAATAACCAACATTTGAAGTAATGGTTTCTACTTTAACTAATAAATTATTATCAGCTAAATATTGACCAATTTTTTCTCTTGCTTCTTGACGAGTTAATTTGTGGAATTGACTTTTTGGACAGTTAATTAAACCGTCTTTATCAATAGTTTCATTAATTTGTAATTTGTGTTTTTTGATAATGTCAATATCAACTTCAGCATGAGCTGATAATTTCATTAAGCCACTACCAAATTTCATGTCTGCATATTCATCAGGCACAAATGGAATTATTTGTTTAGTTAAAGGATGAACAATTTGAATGTCTTTAAAATCTTTATATCTTTTGTCTTTTGGATTGTAAATAACAGCAACGTCACTAAGCATAGTTTCAGTTCTAACTGTGGCAACTGTTAAATATTTATTAGAATTCAAGATTGGATACTTGATATAGTACATTTTTTGTTCAGTAGGTTTATTAATAACCTCAATGTTACTTAGAGCAGTTTTTAAAACTGGATCTCAGTTTATTGCTTTAGTGCCATGATAAATTAATCCCTTTTTGTATAAGGTAATAAAAGCTTTTAAAACTGCTTCATTACTTTTAACATCTAAAGTAAATCTTTCTCTTTCATAATCTAAAGCTAAACCTAACGATGCTCATTGTTTTCTAAACAATTCAGCATATTCTTCTTTTCACTCTCAAACACGTTTAAGAAATTTCTCTCTTCCTAAATCATGTCTGTTTAATCCTTCTTTTTGATAAATTGTATTTTCAACTTTGCTTTGAGTTGCGATACCGGCATGATCCATTCCGGCTACATAAAAAACATCATAACCGCTTAATTTTTTGTATCGAATTATTGTATCCTGAATATATGTATCTAAAGCATGTCCAATATGTAATTTACCAGTAACATTCGGCGGAGGTAAAAGAATTGTAAAAGGTTTTTTGCTTAAATCATGTTTACTAAATATCTTTTTTTCTTTTCATTTCTTTGCTATATCACTCTCTATAGAAGAGTGGTCATAAATTTTTTCAAGCTCCATACTGCTCCAATCTTTTTTAACTAAATTAATTAAAAATTAAACTTTAAAAACTTAATTTTTTGTGCATATAAATGCATAATTATATATATAAATTATAATACAAAACCTTAATTTTGGAGGGCTCTTTTTAAGCAAAATTTAAAACTAATTTTTAATAATTGAGTAAAGAATTACTACTAAAGTTAGATCTTTTTATCATCCATTTTTAATCCCTGTAATTTTTCAAAATCACTATAAAACAATTGTTTTAAAAAATAAACCAACTTTAAAAATGGTCTATTTCTTAAGTTCAATAAGCATATTAAGTTCCGTCTTAAATGCTTTTGATTTTTTGTATTTGTTTTCACAAACTCTTGTGATTCAACCAATGAATCAGAAAATTCCATGAATTGCATAACCTAAAACAAAATTAAATATTAAATATAACAATGTAGCTACATAATAATTTTTGCTTCCGTTAATATGTAAAATATTTTCAAACATTTTAACAAATGAAATCTCTTGACGATGACTCATTAAAAAGAAGTCAGGTAAATAATTAGGGAATCATTCTAAGTTATTTTTAGCTGTTTGATAAACAATTTCATTCCCTATAACTGCAAGTGACCATAAAATTGTAAAGATAACAAAACTATTAACAATAAACATTCCCTTTTTGTATTTTGCTCAATTATTAACAACTAAAATCATACCGATAGCTAACATGCAGCCATGATACATCATTGTATGGATTGAAATTAGTATAACTTCATGAAATACATCGCCTGGTCAAAAAATCATAATGAATGCTCCACCATAAATTGTTATGTAAGCTAAAAAGTCAGTTATGATTTCTTTAAATTTAGTTTTCTTTCTTAAAAACAAATAAAGAGGTACAAAATATAAAGGAAGTGAACATATAACAACTGGATAAAGGTTTTGATGATAATCTCATTTCCATTTATATTCTCCTGCTGTATTTTTGACAGCTTTTCCTGCTCATAACATTTGTTTCAATAATTCCATAAAGACTATAATAAAGAATAAAAGTCCAATAGTTAATTTAACTGTTCATTCTTTTCTATTTCTTAAAGCAACACATAAAATGATGCCTAAAATAAGAAATGTTGCTAAAAATGTCAAGTGGAATGCGCTAAAAGCTTCTGGTCTTCCATTTGCAAATTCAGTATTAAATTTTAAATAAAATTTTGACATTGTATCCATGCTTAAAATTATACAAAAAATATTGAATTTGTGTCCCACAAACCTTCTAGGTTTTAAAACATTTACTTTTATGTAATATAATTAGTAATATATAAAAATAAATATAGGAGTTAAAAATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGTTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATACTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCGAGAAAATATGATGAATTTATTGATGTTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGACACAAGAGTTTGATTTCATAGAAATGGAGCTTACCGTGGATCAGTAGATTTAGCAAAAGCAAAAATAATCTTAGATCGCCTTACTCAACAAGCAGTTGAAAAACAAAGAATTATTGAATATCTTGAACAAGAAAATCTTGTTGATAAACCAGAACCTAAAAAAGTAACTAAAAAAGTTATTATTGTTGAAGAACCTAAACCAATTGATCTTAATCAAGAAGCTAAAGAAGCAACATTCTTTGTTCCTAATTCAGATTTAATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTGTTGAAGAAATAATTACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGAACAATAATCACAATGTCAGTATTGCTTATTGCCGCTCTTACTATCTTAGTTTTAGTTGCAACCCGAGTAATTTAATTAATTTTTTGAAAATAAAAATGTTCTTATTGAAAGGACATTTTGTTTTTGTTTTCTGGTATCTTATTTGATACTTTTATAATTATAATTAAGCTATATACAGCTAAAGCTAGAACAAAATTTATTACTGGATAAAGCAACGTAATTACTACTCAGTAGTTGCCATTTAATTTAACACTAAAAATTTTCTCAAATAATAGTGTCAAATGATTATTAAGACGATGACTAATTGCTAATAAATTTGGATAATTTTCTTGTAATTTATTTAAGTCTTTGTGTGCTGTTTGATAAAAAATTTCATTCAATATTACTACTACACTAAACATTATTAGAAACATAAAAAGATTATGAATAATGTAATTTCCTTTGTCGAATTTTGAATAATTATTAAAAATTAAAACAATACTAATTAACATTAAAATTCCATGTTGAATCATTGAGTGACAATCAAAAAATATCGATTTATTTAAGACTTCATTTGGAATAAATATCATTATTGAAATTCCGCCATAAAGGCTAGTAAATGAAACAAAATCGATCAAAATTTTATTTAGATTTTTGTTTTGATTAATCAAATAAATAGGAAATAAAAAGTAAGGAATTGAACAAAAAAATAAAGGAAAATAAGATCATTTATATTTTGAATATCATACATTATCTTTATCTATTTAACCTAAAGCACTTAGTTGTTTTATTATTTCAAAAGTTACTAAAATACCTAAACAAATCAAAATAAATAGCTTAACTTGTTTATCATTTTTGTTTTTAAATTTGTAACAAATGACACCAGTTAAAATGACAAAAATACCAATCACGATAAGTAAAATCATCCATAAGCTTGTGGAGTATTTGAGAAGGATCACGATATTACTTTATAAAAATTTGCCATGATTTAACAACCTTTGTTCTAATAATATTTTACTAAAAATCCTATAAAACAGTTTTATAGGATTATATTAATATATGTCATCTGATAATACTTGAGTCATTGTCAAGTCAGTTTCAACTTTTTGATTACTATTATTTACTTTTTGAATAGCTTCTAATTCTTGAGTAAATGTTTTATCCAAATTTTTGTTATTTTTAATTTTATTAGGCTGGAAAGCAGCTATCATAATTGTCTTTTCTAATAAAGGATTCTCTAAATCATTTATTTGTGTTTCAATAGTTTTTTCTTGACTCAATTCATCAGTTACTAATAAAGAATTATTTTCATTTTTATGGCCTTGCATTCATAATTTGAAATATTTTGAAGGTGTAATAATGTAGTGCTCTTCATCTACATCATAAATATCACTAAAAACAAATTCTTTTTGACTTCTAATAATTTCTTGTGAAATGGCTTGGTCATTAATTTTACTTTTAATTTCAGGGTTAATTGTGTTATTTAAAACTTCTTTTAAATCTCCAAATTCAACTTCTTTATTATTAGCAATCAAATAGATATAGTCAAACTCATTGTCTTCAACTAATTCTTTATCTTGAGTAACAAAAATAATATTTATTTTACTTTGTTCACATAATTCAAAAGCAGCTCTTAAAAATTCAAATTTAAGTTTTATATCTTTAATATCAGGATCATCAGCAATAATAATTAGTTTTGGTTTCTTAATAATAGAAGAAATAAAGTTCATTTTTAGTTTGTTAATTTTTGAAATATTTTCATAAGGTCTAATTAAGCTCTTTGTATTGATTGATGATTTTTCAAAAATTTCAATTAATCTGTCAAATTGGTCTAATTTGTGAAGAATTTTATTAGAAACTTTTTTAGCTAAAACTAAACTATTTTTAAGAACAATAACATTATTAAATACAAAGTCAAACATTTCAAATTTTTCAATTATTTTCTTTAAATTAGCACTAAAATTTTTAATGTTTAATTTGTCTTTTTCATTAATTGAACGATTAAAAATGCGTTTAAATCACAATGAAGTTTTTAACTTCATGATTTTTCTTTTTATTTTTGAATTAATCAAAGTCATAGTGTCATAACTGTTTAATAATTTTTGATTTGATTTTAGTGTGTTATAGAATTCTGATTGAATTTTGTTGAATTTTCAATTATGTTCACTTTGAGCATTAGTTAAAATTTCTTTTGCAACTTTGATAGATCTTTCAGGGACAGTATTGAAGTATGTTCCATATGATCTATTTTTAATAGACATTATTTGACTTTGAATTTGATCTTTTGAATCTTTAATTAAGTCAAAATTGCTTTTTGATTTGATTTTATAACTTTCGACATTAAAGTAGAATTCTTCTTTAAGAATCTTTTTAATTTTTTTAATTGAAAAGTTTTTATTGCTGTAAGTTAAATTTCCTAAACTATTGCTAATAAATAATTTGATGGTGGTATCTAGTGTTCTTCTCATTTCATCAAGTTCATCAATTTTTAAATATTTAAAATATTTTTTATATTTTTTAAGTAACTTGTGAGCAAACATTTTAACAAAATATCTTTTGTTTAAAATTTGTCTTTGTGAGCTTTCTTTAGCAAAAAAAGTTGATTTATACAAATCAATTTCGGCACTTCTTTTATATCTAAAAGCAAAGTATTTAATTTTTTGATCTGAGTTGTGACAAATTTCTTTTGCTATTTCATCTAAAGCACAAATATCATGTTTTAAGTCTCTAATTTTTAAGTTGTCTTCCACTCTTTTTTTAGAAGTTTTTCTCATTTTGGTTAAGTAGTAAAGTCTCTTTTTTAAGTGATTTATTTGTTCATCACCCAAGTAGTCAATATTTGATTTAGATGCATTATAAAAGAATTCAAATTTATTAAATACTTGAGAAAACAAGTCTAATATTAAAGTAATTTGTTCACCAAAAAATAAGTCTTGGTAGCTTTCAATTTCTTTAAATCTTGTTTGTAATTCATCAAAGTTATATTCAGCAATATTTTTTGTTGTTTTATTTAAAAAACTATTGAAGTTTCTTTCAAAATCACGAAAAATCGCCATTAATGAGTTAGCTTGTTCTACTAAAATATTCAAAATAACATGACGAACAGGATAATAGTATTTATTAACTGTCTTATTTAATTGGTTAATACGTTCCTTGTTTTCACCGTAATTTTTTTTAAGTTCTTCATATTTGTAAACTAAAGGATATTGTAAATTTTGTTCTTCTTTTTCAGCCATATTGAAAAAGTTAATTTCACTAAAAGCATTATTATTTTTATTGAATTCAATGTTTCAATAATTTGTTCCTGAACCTTTTTTATTATTCTTAGAAAAGAAAGATAAAATGTTCAAATTGTTATTGAAAATTGTATTGTAAAAATTGGAACCAATTAGGTTTCTGTTTTTATCAGTAACTAAAATTGCAGTTTTAAGTTGATCGTAAATCTCAATTTTTTGTAAATCTACATAATCATCTTCAAAATTTAGATTCTTAAAACAATTTTCAATTCCAAATATTTTATTTTTCTTCATATTAATAAAAAGTATATTAAAAAAATGACATTTTTATAGCAAAATATTATATAATATTTTTTGCATGGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGATATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCATATGCTGGCTGAGCCAGCTTTTATTTTTTTAAATTAAGCTGTTTTATAGGCTTATTAGTCAAAATAATAAATAAAAAATATGTTAAAAATTTTTAATAAAAAATTTATAATTTATTTAAAGGAGGCTTATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGACAAACTTATTGAATAAAAGCTTCTATTTCTAAATTAAGTATTAAAAATCATGCTTACTTAAATATTGTTGATGGCGAATATTCATCTAATGCTGCAATATGAGCAAGCAAGATTCCGAATATTGGAATTGATATAACAAGTAACAATAAAGATCTTTATAGTCATGCTTATTTTATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGCTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAAGAATTAGTTAAAAGCAGCAAAGAATTATTCTTACAAAAAATACAAGCAAAATTTGAAATGACTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAAGGAAAAATTATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAATATTAGTCAAATTGAGTTCTTTTTTAGGACTTATTTTTTTAACTTTTTAGAGCTAATTTTTTAACCATTTTTTATTTAAAAAATAGTTATATATTAATATTAAAAATTATTTTAATTATTTACTTATTTATAACTATTCGTTTATATATAATAAATAAATATCAGGAGGGAAAAATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGTGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGAACTAAAGAAAATGATGTTCAAATTACACCAAAAATGGCAGAAAACTTGGCAAAAGATTCTATTAAATGACTTAATGTTTATAAACATAGAATATTAGATGCTAATGGCAACTTTATTAAAACTTGAAAAAGTAATGCCAAAAGAGTTGAAGAAATTTATTCAGAAACAAATATTTGACCTTTATTTATTGGGACTTATTTTGTTAAATATAGAAAAGATGAGGTTTGTTTATATGCACCTTATATTTTAAAGGCTGTAAAAATTACAATTAGCAATCAATCAGTTTTCTTAGAAAATGCTGATCAAAACATCATTATTAATGAAAAAGTTAAATATTTACTTGAACAACACAGTCAATTTAATTTACCTATTTTAGTTGACGGTGAAGAAGTTACTTTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTACTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTGTTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGAAATGTATCGAAAAAAATCATTAAATTCTACAATAAAAATTTAAGTAGATATGACATCAATGATCTTGTACAAATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCAGCCAAGTAACTGATTTAATACTCGTGATGAAAATGAAGATGAATATAGTGAAGAAGAAATTACCTCATTATTAGATTGAGCAATTCATAATCACTTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGAGGGTCAATGAGTTGACAATAAAAATGAAGTTGAATGTGAAAAAATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGATTAAACTACATTCAATTAAATGAAAACAGACAAAAAGAATACGCAAATGTAGTAGCAGGCTTAATTTTTAATGATAAAAGCCAATTTATGTATGAGTTAGAAAAATGAATTAAAGAACAAAACTTTTCAAATGAAATCAAAGTTGTCCAAGATTACTGTGTAGGTTCTTATAACATTGATTTAGCAATTTTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAATTATTAAATTACTAGAGTTTTATAGTGCTCTAGTTTTTTTAATTTGCAATTTTAAAAAGTGAAATTTAGGCCTTAAAAAATATCTTTATGTATATTTTTTGCTTGTCTTATTACCTTTTTAATATATACTTTTATTATTGTTATAATAATAAGGGACAGAAAATGAAAAAATTTGTTGCTAATTTTGTATTAAACAATTGAGGAGGATTATCATCAAATCCTGCTTTTAGTAACAAATATGTCGAGTTCATTTATCAAAAGTAATTAATGCTTAAATGTGCTCAAAAAAGGCACATTTTTGTTTGCTTTCTAAATAACAATGCAATATCAACGAAAGGAAAATATGAAAAAACCAAAATTCAATTTTCGTACTCATGTTTGACCTAAATATGTAATTTCTGCAGGTGTGCTTGTTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGATTTATGACCAATGTTTCTTGAGGCCCAGAAATTATTACTCTTAAATCATTCTCAATTGTTAAGGGCGTTGAAATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGAGTTATTGAAGGTAATTGAAAAGAACAATTAAAACAATACACCTTAGATAATATTGAATTAAGCAGATTAATGTGAGCTAATCCATATTTAACTTTAATTCAAGGAAAAGATGAAGAAACTGCTAGAAAGAATTTACTAATTGCTTTAGAAAAATACAAAAATACAGATGAATTTAACATTATTAAAAACATTGCTCAAAATGTTAAATTAGTTCAAAAAACTATTACTAATATTCATAAAGGTTCCAACCCTATTGCTAAAAATGCAAATATTATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGAGTTAAAAATGATAAAGTTGTAAATATTGCTCGTGGTTACAATGAATCACTTGAAAGAATTAACTTATTAAAAGAAGAATTTATAAACAATATTAGAAAAATTGCAAACAAAAATGTTAGCGAAGAAGAAATTAACAATTATGTTAAAGCGGTTATTGCAATTCAACCAACTATTCAAGAAATTACTAAATTAAATGAAACTAAAGCTCAATTAGATGAAGAATTTCAAGAACAAGAAAACTTTAAAGCTCAAGTTGCAAAATTTGAAGAAGAAATTACTGATAAACAAAATAAAGTAGTTCAAAAAGAAACTAAGCTTAGTGAAGTTAAACAAAAAATAACTCAAAAGAATAATGAAATTAAAGATTTAGAAGAACAACTAAAAAGTGATCCTTCAAATCAAGACTTAAAAGATAAACTAGCAAAAACTAAAGAACAACAAGAAAAACTTGTTAAAGAAAGAAATGAATTATTTAAAGAAATTAATAACTTTAAAAACAATATTAATGCAACAAAAAAACAATTACAAAATGTAAAAAACAAGCTTTGAAAATTTATAACTCAAAAAGCTAAAGAAGACAAACTTAAAGAAACAAAAGAAACTATTGATGCTAAGGTTAAATCAACTACTGAAGAAATAGAAAAAGATTCAAAAATTGAAAAGAATCAAACACCTGAAGCCTTAAACGCTTTAAGAAGTTTAGCACAATTAATTCATGATTTTTATGAAGAAAAAGATGTTAAATTACATAAACAATTAGATGAAAGCACAGAAGATTTAGTTGCTCCAGCAGGCAAAAATATTTATACATACAAAAAAGAATTAGCAAATAAATTTGACCTAATTCATTTTGAATATAATGAAAAAATCAAAAAAATTACAACTCCTTATTTTGATGGAAAAGCCTATGAAACAGTAAAATTTGTTCCTTACTCATATTACACAACTGATATTGCATACTTACCAAACCAACTAATAGCTATTGACTCATTAGTTGATATTTCACGTCTTCAACAAATTAATTGAAGAAACTTTTATAACTTAGATGGTTACATTCGCAGCAAAAATATTGATGAAATTAGTAACAATGATGGCTTTTATGTTTATACAAAAAATGTTTCATCACTTGAAGAAGATTTCAACAAAAATAACCCAGATTTAAAATACAAAATAGAATATTCAAAAACTATTAAAAATAGAAAAGAATTACAAAACAAACAAAAACAATTAATGGCTTGAAATACTGAATTTACTGAATTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAACAATGAGAAGAAACTTACAATAAAGTAGCAAACAAATCTTGATATATAAGTACAAATCAACTTATTTTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGCTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGAGAAGATCTAAAAGCAGAATACACACCTTTAATTAAGCCTTACAATGATGCTTTTGAAGTTGATATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGAATTAATGAAATTAATAGTAAACCAATTTGAAATATTGATAAATGAGTTAAAACACTTATCAATGGTCAATTGAGAAGAGCTATTAGAGAACGTACTGATTGATTTAAAAAGGAAATAGAACAAAAATTAAATATAGCAAAAAAAGCTGAAAAATTAGATGAACTCAAAGGAAAAATTAAAAAGGCAGAAAATGAATTAGCGCCTATTAAAGAACGTTTTGATACTAAAAAAGCAGTTTTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTCTAGCTAAAAATTCAGAAATTGGTAATATGATTCTTGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGAATAATAAAAAATGTAAAAGGAATTCATTACCAATTAGAATTAGATAAAAAGGCAAAATTAGAAGAAATTGCAAATCAACTTAAGAAAATTAAAACCTTAACAATTGATTTAATTAAAAAAACAGAAGATATTCTTGGCCACAAAGATGTTGTAAATTGCATTGATAAAATCAATTCATTATTCAATCAATTTGTTATTAAATCATCAGGTTTAATTTCACCTAAATATATTAATGATCCAGTGATTAAAGCTCATTTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGAGCTAAATTTTGCTACTTCACAGCAGTAAAAGTTACTAAAGATCATTTAGATAATCAAGATGCAAATCAAATTAATAATTTAATTTCACAAAATAATGCTGAAATTAATTCTATTAACAAAAGAGAAATAGAATTAGATAAAATTATTAAAGAATATGATCAAAAAATTAAAGCAACAACAGATTTAAATGAGAAAAAACGTCTTAATAAAGAAAGAGGACCTTTTGCTCTTGAAAGAGATAGAATCAGAAAAAAAAGAAATGAAATATCAGCTCACAATACGTATTTAAACAATAGACTTAAAGCTAATTTATTTGATGCCTTTAATAAAGCTAAAACAACTTTTGGACCTTCTGAACTTTTTGTTGGAGCAGACTTACATTTTGATATCACTAAAGCATTAAACAAAGTTCAAAGAATGAGATGAGAATTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGACTTGAAGGTGAACATTTAGTAAGCAAATTAAATGAAAAAGGTGAAATTGAATATACAGTTGAAAATGGTAGATATTTAGGTTTCACAAAAGATAGTCGTGTTGGTTTATGAGCTATTTTAAAAATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGAGGTACTAAATTAAATAAAGACGATATTATTGCAACACTTAAAAACAATAAACGTCGTTTCTTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGGCGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGAGATAAAACTGAACAAAGATATGTCTTTAAACCAGGAAGTTTAGAAATACTTAAAGGTATTTTAAAAGATGGCAAAGGCAATATTGTTGAGTTTGAAGAAGTTAATGGTGAATTATTACCTAAAGTTGTTGAAGGCGTTAGAGATGCTAAAGGTAATTATGTAATAATAAACAAAGTTTTAATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGAGTTGAATTAGTTAATAATTTAACTTATAAAGCTTTTGTTAATAATAGAAATAACAACACAGGTGAATTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGATATTCTAAATTTACACCTGAGTTTATTGGAAATAATAAAAACAAACATTTATGATTTGCAAAAGACAAAAATGGTGAAACATTAAAAAAATTACCAGCTTTAGGTGAAATTCATGAAGTTTCAAAAATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGAAATATTGATTATGTAAAAGGCAAAGTTGACTTCAGTAAATTTATTGCTGGTTTAAGGCTTGCGATAGATGAAGATCAAACCCTTGAAGCAAAAGAAAAAGAGGCCTATACAAAACTTATTCATAATAATAATGAGCAAGAAATTGCAAATGAAATTATGTTTAGATTCTCGCATTCTAATTTAGGTATGTTAATGTCAAATATAACTATTCAAGATCTAAAAGATAATAATGATTATGGTTGAATATTTGATCAAAACAACGGTTATGGAGATTTAAGTGTTTCAACACTCAACACAATTAACCCTGACATTAATAAATTTGAAATATCACTTGAACAATACATTAAAACATATGAAGCAAAAGCTCAAGAATGAAGTGCTCAATTAAATCAATTTAACTTATTTGATGCCTTAATTTTTGATGGCAAAACATCATGTTATACTTCTCAAACAGTTGGAAATATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGATTAGACTTATTAATTAATTTTAGAGGTGACAAAAATGGTTCTGGTGTTAGAGATACAATTTTAAACTTTGAAAAAATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGATTCAAAGACCGTTGATATCGTGATTTCTTAGACTTTAAACTTTATGATGATCAAGGTAAAGACATCAAAGATGATACTATCAGAATTAAAGATTTAACTGGAACAGTAGTTAAAACAAGAGCCAGAGCTTACTGACAATATTACATTCAATCTCAAGGCGTTGGAAGAAGAAATATCACCAGCATTTGAAGAGATTCAGATAAAGATGCAGTTGCAATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGAGTTTCAGATTATGCAATTATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGAGATTCAATCTCAGAAAATGGCAAAACTTTCTCACAAGCACCTATATCAATTTATAGAGATGCTGATGGTAACTTTAGACTTAGAGTGCAAGATCAATTTAATGGGGTTATTTAACCAAAATAACTATAAAACAGGAGGTTTAGAAAATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGTGAAAGTAGATGAAGTAATAATGATATAGTAACTGCTGATGATTACATTGATGCAATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGAATTTATGATGTTTTAAATCCTAATTACAAACCATGAGCTTGTCAATTAGAAAATGAGGCTGATAAAGCTGAAGTTGAAAAAATTAAAGAAGAAGCTCTAAAATTAGGCCTTTATTCGGGACGTATGTATTGAAATTATGATAATCAAACAATCTTAGCAGCCATTCCTTATTCACCAGACTTTAACGAAAATGATGAAATTACAACGGTGATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTACTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGAACCAACAAAAGTTATCGTCCATTTATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGAAACTCATCATTCCCAGTTATTACTTGAACTGCTTTTGGTCAAGGATCATCAAGCTTTGGTGATGCTGTTGAAATTGGTTTTGATCATGACTTTATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGAACTTACCAAAACTACTTTGAAAGCTTAGGTTATGTTTGAGATGATAAAACTCAAAAATTAATTTCAAATAATGCAGCTTTAGTTGATGAAACAATTAAAAGACTAAATATGGAAACAAAACAATGAAACAAAATTCTTGATTTAGCATTCAGAAAAACTTATGTTGATCAAAAAGATGCTAAACATGAAACAATTACTAAATTTACTGAAAGATATTTAAGATTCTTTTCAAACCAATTCAATGAACAAGAAAAAGCAGAAGGTTGAACTGAACAAAGTGCTTTTGGTATTATTACTGCTTTAGAAAAAATTATTCGTGATGCAGCTCCGGTTGTACCATTAATGGAAGTTGATACTTACTGAGAAATTTCAAGAGTTAATGGAACAGAAAGCTTATTCACCTACTCATTACAATTTGCTTATGACGTGGCAAAACCTCCAAGAGCAACCTTGCCTACAGTCATAAAATCTTAGAAAGGAGACAAAATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGATGATCAAGAATTTTTAACATTCAATCTCCTGCTCTAAAAGTGTTATTCAAAATTTCATGAATGATTTTAGAGTTCTTACTAATTGCTTGAGTAGTTATAACAATTACATTCTTCTTAATTAACTCTGTGCCTGGTTCAACTACTATTTCAGCTGGACTAGATGATGCAGCTCGGCAAGCAGCTGAAGCTCAATATGGACTTAACTTGCCGCTTTATCAAAGATATTGAATTTATTTAGGAAACTTACTTCATGGAGACTTTGGAGTTTCACTCTCAGTTTTCCCTGGTGAAAAAATTAATTCATTTGTCTGAGTTAGATTCCTTAAAAGTTTCATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCAAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGAACTCATGCTCTTAAACCTTCATTATTCCCTATTGCTACATTCTTCCCTGTTGTCATCTTTGGTAGCTTTATTGGATCAATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAGAAAGGAAAAACTATGAATTGATTTAAAACTTGAGTTGAAAAGAAAAAACGTCGACAAGAAAGTCCAAACAATATTCATGGTAAGGACTTAGCTCCAAATCCTTTCTTACAACCATTAAACTATAAAAAATGAGAAATTATCGGTAACCTACTTGAATTCCATGAAACCTCTTCAATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGAGCAAGACTGTGATGAGGTTTAAGATATTCATTAGCTTTAGCTTTTGTAGCAACATTTATCCAAGTGTTTGTTGGCTTAGCTATTGGTATAATGATGGGACATTTTAGAGTCTTTGATAAAATAATGACTTTCATTATTAAAATTATTTCAAATGTGCCTTCTATTATTATTCTTATTGTTATTACTATTATTCTTAAACCTACATTTTGAGTTATTGTCTTTGCTTTAACATTTACTTCCTGAACTGGAATTGCTAACCAAATGCGTTCACAAGTTATGCGTGCTAAAAACTTTGAATGAGTTAGTGCTTCAAGAATTCTTGGAACTCCAACCTACAAAATTTTACTTAACTACTTACCAGTAGTTGTACCACTTCTTGTTACTGAAATAGTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAATATGGAAACAAAAGAAAAGAACAGAGGGTATTCAAAAATATTTAGAAAGCCTATAGTTTATCAAAACAAACCAGCGCCGCTTGACTTGTTTGAAGTAAAAGGCAATCCTAAAAACAAATTTTGAAGAAGTTTTAAAGATGCTTGAATTAACTTCGGAAGAAATATAGCTAAAGGTACAAGAAAAATTGGCGAATGATTACATGTTGTTAAAAAGCAAGAAAAAAATAAATTAGATTTTAATCAATACATTGAGAACGCACAAGTTGAAGATGTATTCGGAGCACCTACAAAAGTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAGCCTAAAATTTTAGTCATGGATGAGCCTACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCGATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGAGGTTTAATTGTTTCAATGCCTGACTTTAACAAAGGCACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCGGTTAGAAATGACTATGCTTTAGGAATAGATTTTGAAGAAGAATCGCCTTTCTATCAAATTTCTCCTACACACTTTGTTAAAAGTAATTTATTTAATGAACAAGCTCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGAAATAAATACAATTCAAAATTAGAATCTATTTATGGCGCTGACAAATATGTTGATCAAAAAATATATGATAATTTTGTCCAAATAGCCTATGAAAATAATGAATTGGTAAAAATTTTAAATACTAAAAGAGATGCCGAATTAGCTTCTAGAGAAGAAAATAATCAAGCGAAATCAGGAGTAAAAAATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGCACAGAGGAAATGCTTAATGTATATCCAGATAAAAAACCTTTAGTAGAAATAAGACATTTAGATATTGATTATGGTTTTGGAACTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGTGAAGTTTTAGGACTTGTTGGTGAAAGTGGTAGTGGTAAAAGTACTACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGAGGTAAAAAATATAAAGAAATAATTAACTTTATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAACTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACAGGCTTTGATCAAACAGTTTTAAAAGAAGTAAATAGATTGGTAAATAAAATTGATCCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATGACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAAATAATTTAAGTGAAAAAGAATTTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTATACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATTGCCGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAGGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCTGATAGAATAGCTGTTATTAATAAAGGCGTCTTACTTGAAGTAGGTCCAACCGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCTGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGACAAGATTTAGGAAATAATCACTTTGTGCTAGCTACCAATGAAGAATTAGCTGAATACAAAAGAGATAGAGCTAAACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTAAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGATGTTAAAGAACCTAAACCAAAGGTTGAAAAATCTAAAGTCAAAATGCCAAAAGACAGACCAAGTGTTCGTGTAACTCCTTCTTATATAGAAGTAGCTAAAGTTATTGAAGATGAAGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAATCATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAATTAGTAGAAAAACTTGCTAAAGAAAATGAATAAAAAATCGCAAGCAACTGCGATTTTTTTAAACCTAAATTTAAACCATATTTTTAGTTTTTAAAACATAAGTTTTGAAAAGTGTTTTGTCTTCTGAAACATATTTAAATTTAAGTTCTAAATGATTTTTGCTTACAAATTGATAATCAAAAGAAATTTTACTTTGATAATCTGCGTTTAGTTTTTGAAAAACATCTTTCACTATTAAAGGCACAACATTTTCATCAATATATTCTTCGCCTTTTTCACCTATTCTAATGTACTTGTAATAGTTGTTATATTCTAATTTAGGAAATTCTTCAAAAGAATTTATTGTTTCTTGTTTTTCAATTTCCTTATGTTTACTTTTAGCTGCAGATACAACAACAATTGGAGTAACAACAGCTAAAACACCAACAGTACTTAAGAGTGGGATTAAAATTTTCTTTTTCTTAGCCTTATCCATTTTTTACCTTATATCTGTCTAAAGAATAAATTGAAGAAAAGTCTTTTGATTTTAAATATTCTTCAATATCTTCTCCTTTAAATTCATATGCTGAATTTGAATTAAAAGTATCATTATTTTCTTCGTATTTTAATTGAATTGTTCCTTCAACATGATCTAATATTTTTCTCTTAAATAATTGGTGATTGTTGATATTTAAAATAATAGGTGAAGAAGTATTTGAATATGCTTTATCATTGAAATAAAGAACATAATTTGTTTTTAAGTTACCTTTGTAATTATATGGAATAAATAGACCTGCTTTTTGACTTTTTGTTGAATTTACTAAATAACCATTGTCATAATCATAATCTATGAAATTTTCGTATGTGCCTGTTTTATAGTCTTTTCACTTAGTTGAATCGTCACTACTTGTTGTGGTTTTAGTGTAGATTTCATTCTTAACTTTTGTAAATAATTCATTTGCAATATTCTTGAAGCCATAATTAGTTAAATTTTGAGAAGCATAAAAACTTTTGCTAATCTTTAAAAGATTTAATAACCTTGGGAATTGTTCATAATATTCTTCTCATGTAATTCCATGTCAAGGTGCAGTATAACCATAAGGTCAGTGATCTGATAAACTACTTTCTTTTGCTTCTTCAGTCTCAATAGTTGCTAATTCATTTTTTCTTTCATATCTATCAAATTCATTAAGTTTAACTATTGAGTTTAAATAATGAATTTTAAGTTCATCTTTTTCTATGTGACTATTGTAACCATTGAAATTAAATTCATATAGTTGAGGTATTTTGAAATATACCTTATTGTCTTTAGCATTGAAAGTATATTCTCTTCTTTCATCATTGATTTTCTTGATTTCTCAATATTGTTTTTCTGTTTTTTCTTTACTTCTAAAGTAAACTCTTAAAATACGGAATTTTTCGTAAGGTAAATTTATAATTTTAGGTGAAAGGATTGTTAATCCATATGCACTTGTTTCTTCAGGAAATTTCGAATACCATTTTTTAACACTAAGTCTTTCATTTAGTAAACTGCTATCCATTGTTTCCAATTTTGTGCAGCTTCTAATTATGTTTCCATCAATTCTTTTTCCATTTATTTCAATATCAAAACCTTCAAAGAATTTTTTATGTAAATTTTTGGGTTGAATAAAAAATATTTTAATTGCAAATCTATCTTCTGTAAAATATCTTTTTATTTCAATATTACCATTTACAATATTGCATGCATCTTTAAAATTTACATCATAGTGATCTTTAGTTAAATAAGATGGAGCATCTAAATATTCTGATTTGTATACATAACGTTCTTGCGAAATATCATGCACCAATTGTTGTGGTGGTTGAGGTGGATTATTCGTTAAAATAATGGTACAAAAACTCCTCTACTGCTTGTTTTTTATACTTATAATAAGTATTTTTGGAATAATAATCTTTGAATCAATTTTTAGATTGACTTTTTAAGAAATCCTCTTTGATAATTTGCGCAAGTTCAGGTTTCATAATAGATAAAATATGCTGACTTTTATTTTTTGGTTTAAAGATGTTTTTAAGTATGTTTGATTTGTTGCCTCCTTCAATTGTCTTTTTGTAAGCTTGCTCATTGACTTTTTCCATTTTAAAGATATTGTGTAAAACACTATTTTGATCTTTATGTGAAATTAAGCCCATAAAATAATCATTAAAAGTTAGATTCATTTTCCTCCTTTCAATTTATATCTTTTCAATATAAATAAAGAAGAGTCAAAAGCGGAAAAAAATAGGAAAAAAGCATGATTTTTCATAAATTTTTCCTATTTTTTAATTTATTTAATAATTTTGTACACAATTTAAAGAAGTTGTACGCCTTCTTTAGCTAAATCATCAATTAATTTTTTAGGTCAAATACCTACTTGAACTTCACCAATGTGTTTCTTCTCTAACAAGAACATTGAAAGTCTACTTTGCCCTATACCACCGCCAATAGTTAAAGGGAAAGTATTATTAATAATATTTTTATGATAGTCTTTAAATTCATCTTGAGAAACATGACTAAATTCAGCTTGTTTTAATAAGGAATCTTTATCAACTCTAATTCCCATTGATGAGATTTCTAAAGCTCTTTTATTAACTGCATCATAAACAATTAAGTCACCATTTAGTTTTCAGTCATCGTAGTCAAAAGCACGTTTTGATTGAGGCTTATTATCTGCTAAAGGATAACCAATTTCATAAATAAATATAGCCTTGTGTTTTTTAGCAAATTTTGTTTCTCTTTCTTCAGGGCTTAATTTTGGATATAAGTTGTAAAGTTCACTACTACTAATAAAAGTTACATTTTCAGGTAATTTTTGACTTAGTTGTGGAAATTCAACTAAAAGTTTGTATTCAACATGACGAACACATTTAAAAATTGTATTGACAATTTTTTTCAAAAATTCAAGATTACGGTCTTCTTTTTTAATAATTAATTCTCAGTCTCATTGATCAACATAAAAAGAGTGTAAGTAATCTAAGTCTTCTTCTTTTCTAATTGCATTCATATCAGTTCAAATACCTTCATAAGTACTGAAGTTGTATTTCTTTAATGCATATCTTTTTCATTTAGCAAGAGAGTGAACTATTTCAACAGTTTCATCAAAATTTTTAGGAACAAAAGACACAGGTTCTTCACCATTTAAGCCATCATTTATTTTGGTATTTCTAGCAACAAATAAAGGGGCTGAAACTCTAGTTAAATGTAATTCCTTGTTTAATTTTTTAGTAAATGCAAACTTCAAATCTTGAATTGCTAATTGAGTTTCTTTAATGTTTAATTTACTCTTGTACATTTTCGTTAGCCTCTGTTTTATAGTTGTTTTGGTCATTTTGCGGAATTGAATTTTCTTGATTATTTTTAGCTTCTTGCATAGCTTTTTTTGTTGTTTTCAAGATGTGTAACATCATTGATAATTGTGATATGTAAATTAACAATGACAATGTTTGTCATAATAGTGTTGAAGTTAAGGCTGGAGTTAATTTTCCTTCTGCAATAATGAGTGATACTCAGTATGCAATTCAGAAAATATTTGTTAATACAAACATTAAAATTAAACCAACTGATAAGCCTTCAATATCTCTCTTTTCAAATGTTTTTAATACTTGTGGTAAGAATGCAAAAGTTGATATAACTGGAATAATTTGAGACAAAATAGCTGTTGTATTAGGATCAAAATTAAGGCCATTGCCTTCACTGTTTTTGTGATATAAACCGACAAAACCAACAATTGCACAAATTGTAGTTAAAGTTAATGTTATTCCTAAAACTACAAATTTTCTTTTGTTTCTTTTTACTTTTTCATCATATTTGTACATAAAGAATAAAAGTACACAATAGACATAAGCACACAAAATATTGGCATAAACTACAGCCGCCATTTTAGCTTCTTCCCCTGTGAATGATCCAAATATTATTCAGCTACATAATGCAGCAAAAAATATTCAGAATGAATAGAATTTAACCTCCCCAGTTTTCTTTCTTTTTAATAAATAAACTAATTGAGGAATTGATACACTTATTGTTGCAATACATGCTATCATTCCTACTATTGCTACAAACACTGCTATGCCATCATTAACATCTTTATTTATAGCAAAAGAACTACGTATAAAATCCATGATTCAATTATATATTAATAATGTTTATAAAAAAAATAATTGAAATATTAAAAAGATAACAAACATTGATAAATAGTAATAAATCAATTTTATGTTGGTTTATTTTTTAAGTTTTTATTATTTTTCTAAAAAATAATCATAAATTTATTTGGTAATTAAATCGCATAATTTTCAGTTTTATCTACTTTTATTTTAAAATTAAACTATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTCTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAACTTAAAGCTTTTTATTATTATATGGATTGTGAATGTAGTCCTTGCAAGCCAAACAAAGAATATGTTGAAATGTGTTTAAAAGGATACCAAGACTGCAATATTAAAAAAGAACAACTTAGAGAAATTTTATACAAGTAATATACAAGTAATATACAAGTTGTGTTATAATATTTTTATGATCAACAAGGAATATAAACCTGTGTTCAAGGTTACAAACAAAATAATAGAATTGGTTGGCAAGATTAAAGAAAAATTGTCAATCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAATGCTTTAAAAGCCTATAAAACAATTGAAGAAGTTAAAAATAACAATGTAGAAAATTTTTTAAAAATACATAAAATTCTTATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGATACAACAATGATATTAGTGATGTTTTAATAAAAAGTTGTGCTTTTCATTACGAATTAGAATTTATTCATCCTTTTGAAGATGGTAATGGAAGAATAGGAAGATTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTCATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATAAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGTCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTGGAACAGCAAAAAATAATGATTATTGGTAAATTAAATTCACCTAAAGTAAAATATAAATTAGTAGAAAACAATAAAGAGTAACTAATGAGCAAATTTATAATTGATAAAAGTTTTTCGAACTATTTTCTCAAGTTTAACTTGGAGCAGTTTTAATTAATGATATTAAAAGTAATTCAAACAGTCCAAAAGAGCTGCAAGACTATTTAAGAAAAAGTACTTGAGTAGCTAAGGAGACCACTTAACTTCCACATTTTTTTCAGACAATGAAGTTGTTAAAATTTATCGTGAGCATATAGTAAATTTAACTGTATGAAAAAAACAAAATCAAGTGTTTAAATAATGTTTAAAAGAGCGAGTAAAGATGCACAATTTTCTTCAATTAATCCTTTAGTTGATATATAAAATAGTGTTGGCTTACATTTTGCATCTCCTTGTGGCGCAGAGGAATTAATAAATTTAGTGGCAATTTAATTTTAGACATTGCCAACCGTAAAAATGAATTTTATAGAATTAATAGCACTGAAAATGAACTTGCTTTAGCAGGAGATTTATGTTATTATGATGATAAAGGTGCTGTTTGTCGTAATTTTAATTGACAAGATGGAGAAAGAACCAAAATTGATGAAAATACTAAAAATGCCTTTTGTGTAATTGAATTATTAGATACTAATAGATTAAATGATTTAAATGAAGCACTAAAAACATTAGCTCAATTATTTAATAAATATTTAGGAGCAAAATGTACTATTCATTTATTAGACATTAACAATTCAAAAATTGAATTAATTTAACAAATTGTTATATTTGCACTTAAAATATGTTTAAAATAAGTGTGGATATAACACTTTTTATAATTTTTTTTGTTCTACTTACACTTAAATGTGATAAAATTTAAGTGTGAAAATAACAGGAGGTAAAATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAAAAATAAATTGAAATATTTGTGTTCCTTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGAAAAAAATATTTAATCAAAAATGAATTTACTTTAAATATTACTTTTAAAAATTTAGTTTTAATGATTGTTGAATTTTTAACTGAAATTTTTGAATAAAAAAGAGTTCACCATTTAAAAGTGAGCTCTAATTTTTTTACAACTTAATTATTTAATGTTGTAGAATGCTTCACGACCTTCAAATTCATCAGCACCATCTAATTCTTCTTCAATAGCTAATAATCTATTGTATTTTGCAATTCTATCTGTACGTGACATTGATCCAGTTTTGATTTGTCCTGTGTTTAATGCAACAGCAACATCAGCTAATGTTGTGTCTTCTGTTTCACCTGAACGGTGTGAAACAACACAAGCCATTCCAGCTTTTTGAGCCATGTTAATAGCATCAATTGTTTCTGTTAATGAACCAATTTGGTTTAATTTAATTAAAATTGCATTCATTGCTTTTTCTTTAATAGCACGCGCTAATAATTTAGGGTTTGTAACTGTTAAGTCATCACCAACGATTTGAATTTGGTGCCCGTATTTAGTTGTCATTTTTGTAAAACCAGCTCAGTCATCTTCTGCTAAACCATCTTCGATTGAGATAATTGGGTATTTAGCAATTAAAGTACCATAGTATTCAATCATTTCATCTGTTGTGTATTCAAGTTTAACATTTGTAAGTTTTTCAAAACCTGGGTGTTTAGCTTCAATAGCTTTTTTAAGTTTTTTGAATACATATTTTTTGCTTTTTTCGTCGTAAATTTCACTTGAAGCAGCATCCATTGCAATAGCAACAGCATCTTTACCGCTTGTAGCTGGTTTGTAACCTGCAGCCTTAATAGCTTGAACTAAGTAGTCAAGAACTTCTTCATGACTTTTGCAGTCAGGTGCAAATCCACCTTCATCACCAACTTGTGTTCCGTGTCCTGCAGCTTTTAATAATTTTGCTAAGTTATGGAATACTTTGTTTGCCATTTGTAATGCTTCACGAATGCTTTTAGCACCAACTGGCATAATCATGAATTCTTGGAAGTCAACTGTGTTTGATGCGTGAGCTCCACCATTCATAACATTTAGCATAGGTAATGGTAATACGTGGGCATTTGTACCACCAATGTATCTATATAAAGGAAGTTGTAATTCATCTGCGGCTGCTCTAGCTACTGCTAATGAAACTCCTAAGATAGCGTTAGCACCAAATTTTGATTTTGTTGCTGTGCCATCTAATTTAATCATTTTCATGTCGATTGCACGTTGATCTGTAACTTCCATTCCTAATAATTTAGGTGCAATATCTTTGTTAACATGATCAACTGCGGTCATAACACCTTTACCACCAAATCAGTTTTTTTCGTATTTGGTTCCTTGGTCTCTTAATTCAAGAGCTTCTTTTGAACCTGTACTTGCTCCTGAAGGAACATTAGCTAAACCATATCCGCCTGCTTCTGTTCATACTTCAACTTGAACTGTTGGGTTTCCACGTGAGTCTAAAATTTCACGTGAATGAATCTTGTAAATTGCTGACATTTTTCTCCTTAATTTTAAATTTTAATTTTATGTTTTATATTATACAAAAATATTTATTACTATTAACAATGAAATTTTGCTAACTATTTTTTATTATGGAAATATTACTCGTAATTTTCATTAATTACAAAATGTCAATATAATTGTTAAAAATAGGAATTAACAAAATAGATAAATTTATTTTGAAAAAAATCTTAATATATATAATATTGATATTAATTAAATAGGAGAAAAATGGCAAAAAACCCAATTATAAATAAATGAAGCAAGAACTCATCTAATTATGATGACTTTATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGAGGCGATGGTAGACCATTTAACCAAGTTGAAATTGCGAGAATTTTATTTAAATTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGAAAATCTTTACTTAAGAAACCAGATGCCAATTTTGCAATCAGTCAAGATGGCTCAGGAATTAATCTTTCTGTTCGCTACAAAAAAGTTTTTAAATATTTTAAACCTGATGAAATAGCGGCTTTATATTTAAACTTTTTAATAGAAGATGATCCAACTTTTGATAAAGCAAACTTTGCTAATAGTTACATTATTAAATCTCTTGGAGCTGGCAGTTTAACAAGTTTAATAGCTTTAAAACATAATATCGGAGTAGAAGAAACTCCTAAAGTTTCTGGAATTTGAGAAATCGCTAATAGAAATAGTATTAAGAACAAAAAATTACTTTTTGCTTTTACAAGATTTAGTCAATTTGCTCCTTTTAATCGTTTTTTCAATGGCTTTGACGCTAACTTATTTATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACAAATGTTGTAATTTTGGAAAGAGAACTTATGGATGGAATTTTAGAGTTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGAACATTTATTAAATGATTCCTATTTATTATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTTAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGAAATGATCTGATGATTAGCTCTCTGATTTCGACAATAGTGCAAAATGTTACACCTAAATCAATTGGTGGCGATTTAGCTACCTATTGATACTTAAGAAGAAAAGGAGTTAAACGTTCAACACTTTTACCAGTTGTCGTAACTAATACTTTCTTATGACAAGTAACTAATGTTATTATAACTGTTTTCTTCTTACCTTTTGGTATTTGATTCTATAAAGATTTCTTTACTAATATTGATAATCCACAAGTAAAAGTTTTCTATGCATTCTTTATTACAGGATTATTCATGGACTCAACTTTTGCTTCGTTCTTCTTCATTTTGGCATTTAACAAAAAAATCCAAAATTGATTATTAAAAGTATTTATCAAATTCCTTGAATGACTACCATTTGTTAAAATTTATGACCCTCTTTTAGTTAAGGCTAAATATGAATATGAATTTTATGAAATGCGGCAAGGAATGAAGAAAACATTTAAAAAATGATATCAATTCGCAGAAGTAATAATTTGAAAATTATTGCCTAACTTCTTCTCGCCAATAGCCTTCTTTATGAAAGCTTCTGGAGTATTGCAAACTAATCTAAAAGGTGGCTGATACTTTAATGTTCTTGTTTCAAATACAATTATTCGGATAGGAAACTCAGTAAGTATAACGCCTGGTGGTCAAGGTACAAACGAGATCTTTACTAATATGATTTATAAAACTATTATGAAGGATCTTACCAATGCAAATGAATGAGTTTACCGTGGAAAAGATTATGGACTAAATCAAATTAGTAACGCTAAATTGATGACAACTATTGGCTCAATTTGAGGAACAGTTTTAGGTTCAATTCTTTCTGCTATTTATTTGATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAGGTACAATAGATGCCTATTTTTTTGTACAAGCCTGTTTTATAGTGCTATCAAAGAAATGACAAGATTTAAAAAAGCAAAAAATTTTAGTCAAATTAAGAAGGCAAAAACAGTTCAAAAATTATTTTTGTAAATTTAATATATGTAAAAGCATTTTATTATGCTAAAATATTAAATGCTATTTATAAATATTTATAAAAGTTATATTAGTAAATAGTATGGATAGGATGCAGATGAAAGGCTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAAATATTACATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGAATTCAAACTCCAGTTAAAGAATTCGACAAACCATTCTTAATGGCTGTTGAAGACGTTTTCACAATTACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACAGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGCACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAATTTAATTACGATACACATAATAATATTTTGAAATTGCAGGGTAGTTTACCCTGCTTTTTTATATATCTAATCAAATCTTTTTGAAACTTTTAATATATAATTAAAAATAATATTTAAGGTACATATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGAAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAACATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCACATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTATTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCCTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCGGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACATTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGCTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGCTAATCATTCTGAAGTTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAAATTAAATGAAATTAATGATTAATAAACATTTTTTTACATCATCTAAACTTTTACAGTCTCTTAATCCTTCTTCTGCCCCCCCTTTTTTTTAATTAAATCAAGAGAGACGGGAGAGGGATTAAGATTTCTTGCATAGCAATAACTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTTCACAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTCTATTAAGAAATAGAGAGACTTTACACAAAATTCTAAACATTCTCTAATTTAACTCCATTACCATCATTTACTTCATTAAATTCTTTTGCGAAATTTTCAGCACTAAAAGTAGTGGTGTCATTGTTGTACTTTTCATTTATAAATTTTATAAATGCATCATATATTTTTTCAATATGTTCATCAAGGGTTAATGGAGAGATTGAATGGGCCACATCTAGAATTTTATATTTTATTTTTAATTTGTAAAGTATTTGTAAATCTTTATTTATTTGTTTTACTATATTTCTTTTAATATTTTTTATTTTTTTAATATCGGTTTCTTGATTTTGTTCAAAGTACTTAAAGAAATTATGTGCTACAGCAGGACTATCTTTAATTTCAAAAATATTTTTATTTTTAAATACAAAAAAGTCTTTAATACCAGGTCAAATATCATTAATGTTAATTATTTTTTCAAAATCAGCAGCATGCATTTTGGTATTTCCAAAACTTATTTTATATTTGTTATTATTATCTACACTTAGAGTTGCATCTTCAAAATAACGCGGTTCTTTAAAGATAAGTTTTGGCAATTCTTTTTTAATTTTTTCATCTATAAAATCTTTTAATTTTATATAAGGCCAAGTATTGCTTTTAACACTTTCATCTTTAACATAATTAATTATAGAATCTTTGATTTTTTTAATGTCATTATATAAATAATATTCTTTTCATTGATTTTTTCATGAATCAGTTGTTTTCATACGTTCTGAGTATAAAAGTATTTCATTTTTTCTTTTTTCAATATAATCGTTGATTGCTAAGTCAAGCAACGTTTCACATTCATTATCTACATTATCAAAAATAATTTCTGATTTCTTTGTGATTGCATATAACACATTGTTGTAATTATTGTTTTCAATTTCGTCCCAATTGAAATATTTTTTTGAAAAATGTCTAAATTTCTTGATTTTTAAAGTAATTTGTGGTTTATTATTTTGATTTGAAACTTCAAATTCAAAGTTTTCGTTTGATAAAGTAGATTCAAATATTGATTTATTCAAGAATTCATTATCAAACAAGAAATTATATATTTTGTCTAAATTTTTTATTATATCTTCGTTAAATAGTAAATGAGAAATACTTTCTTCACTTCATTCCGAATTTTCAAACATTATAAATTTAGAGTTTTTTACATTTTTAATGACATCAAGACTATTAACATCAAATATGAATTGATTGCCACCTTCAAGTAAATTTTTTATTATTTCATATAATGATTTTTTGCCTTCATTAAAATCCTTGAATTCATCTGTAAAAATTCTTTTTAATCTGTTAATCTCTTTTTCGTTTTTGTCTGCAAATAATAATCCATCTTGTAAAATTTGTCTTATTAATCATTGTCATTTAGTAATTTTTTCATCATATTTTAATTCTTTAATTCCAGTTTTTTCACCATTAGAACGAATTTTTCTTTCATTTAAAGAAATCAAGAAAAATCTTTCAAATTCTTTTTCATGAGTATTTGGATCTTCTGCTCCTGTATCATTTAATTTAATATCTTCAACTCTTATAATTTTTTCATCATTTTTTTCATAAGTTGATATATTAGTTTCACTTGATAGATTTGGTACTGTATTACTAATATTTTTAGCCAAATGATTTTTCTTAATCACGTTTAACACATAATAATTTTCTAAATTGTCTATTTTGCTTTGTAATTGGGAAACAAATACATTTCAATCTCCGGCACATTCAAAATAGTTAATACTTTCATATTGATTGTTAACACTATTAAAATATTGGAATTCTTTATATTTTTTATTCACTATTCGTTTATTACCCGAATTATTATTGTAATAATCAATTAAATTTGAAAGTTCAAGCTGAGTGTCTTCATTAGCAATTTGTGTATCGCAAATATATTTTACAAATTTTTCAGAAACTTCATTTGTTGAATTAATTTCGAATAATCCAAAATGTTTGTTTTCATAATTTGATTTGAAAAATAAAGTTAATAATTTTTTTAAGTTTTGAACATCAGTGAACTTAAATTCCTTTGTGTTATTGTCATCGATTTTTAATTTTAAACTTTTTTTCTTTATACTTCATTGAACACTAGGGAAGCTTTGTTTATTAAGAATTATTTTAAAGTTTTGAATTAAATTGTAATCTTTAAATAAATTTTCTAAATCTTTATCAAAGTTCTTTTGTTGATTACCATTAAATAGATTTCTTGCATTAATCAAAGTATATAAATTGTTTTTTATAGTTTTTCATTCGTTTATAGATAGTTGATTACCATCATAAGATATGTCACCTATAGTAGAATCTCCTGTTAAAAATTCTAGCGATTTAACAATTTTTACTTTTTTTCCTCGATAATTTTCATTTGCAAAGATATCACTTAAAGAGATGTTAGCTGTTTTTGAACTATTTGCTATTTTTTCTTTAATTCTGTTGTAAATTTCAATTATAATTTCGTCTTTATTTGTTGCTGTAACTCATTTATCATATTTATATTCTAAAAATCAAATCGAACTATTTAAATAATTTTTTAATTCGTTATATTTTTTAAAACTATCAAAGTCAGAAACACCTGATGGTTTTAATGTTTTTGATTCAAGAGTTACAATTTCTATATTTTTATCTTTGTATTCTGAAGGATAATTTACACCAGCTTTTATATTCAATTTTGATAAATCACATTCATTATCAATTTCAGAAATATAAGCATTAGTCAATTTACTTTGAATATCATTTTTTATTTTTTCAATTATTTCTTTTCTACATTTTTCATAATCTAAATCTTGTAAAGGATATTTAAAGATATTATCTTTTTCTTTAAATAATTCATCAACTATTTTGTTGATATTGTTTGTAGCAAAAAAACCTATTGTATTAAATTCATAAATTTTGTTTACAATATCATATGTTACGCTTATTTCTTTATTTAAATTATTAATGTCGAGATATGTTGTATTATCTTTTTTAAAACCAATATTTTTTAATGAAATATTAAATGTATATTTATCATTTATAAATGAATCAAAAAAATTTGAATTAGTACCGTAAAAGTAATTTGTTATTTTATCGAATTTCTTATCAACTGATAATTTTGCAACTTCTGAATCATAATAAAATGTTTTTTTGTTGTAATCCGCATTTGAAAGTATATTTTCAAAACGACTTATGAATTCTAATTTTGAATTTGAAATGTATTTTTTAATAAAAGTTTGAAATACAATATTTATATTTTTTTCATCTTTTATTCCACAATATTCCTTAACTATATTTAAAGTATTGGCATTGTTTAAATCCGGCATTATGATAAAGTAATTATCGGTCAATTTGAATAATTTTGCAGAAATTCAACTTGTAATAACATTGTTATTTTGATTAGTTATAACACTTAAAGGATCTATGTTATTAAATTCATATTTTCATATAGGCATTTTAAGTTGATTTTTTATTTTATTTCAATTAGTTGCTTTATTTGAATCTACAAAGAATAGACCAAAACTATTTAGGTTACAAATAAAATCACTAAAATAATTATCATCTTTTTGAAGTTTATTTGTTAAGTATCCTAGTTTGTCATAATAATTTAATTTAAATTCTTCATTATTTTTTTTAAATATTTCTTTTGTAAATGGTGTTTGACCATTAAAGTTAATACTTAAAAATTTTGCAAAAGACAATCTAATAGTTTTTGTTGGAATATCTACAATGTAATCAACAATTTTGTAATTTACATTATCTAAATTTGAAGTGTAATATCCAACATTACTGGTTTGTCCTTCGTATTGTGATTTTATAATATTTTTTATATCTGAATATAAAGCATTATTTGCAAATAGTAGATCATTTAATTGAGCAATTGCATTTGAACGACCACCATTGTTTGCTCATTCTGTTAACTGTTTAACAGTAATATCAACTCTGTTTTTAATTCTGATTTCTTGATTGTTTTTTCCATTAATCCCTAATTCATAGATACTTTTTTCATTATCTCTTCCATAAAAATTAATAACTATTTTTTCATATTTTGTATTGTTGTCTTTTGGATTTGAAAAGCTTAAATTAGCATTATATTTTTTAATGTATTCTTTTAATTCATCATTTTTATAAAATGTAATGTAATTTTTAACACTATCTTCAAATCATTCCTTACTTTTATCAATTTCTAAAGCCATTGCTAATTTTTTAAGATCCTCATCATCAAGTGTTCATTCATTATCAACAATTTTTCTTTTGACTTTAAATGTTTTATCAACTACTTTAATTTCAAAGTTATATAAATTAGTGTTTGTGTATAAGCGGATTTCGCCTTCTTTTGAAGACTTTCAATATTCTTTAAACAAATTATAAACAAGGTCATTTTTATCTACTTTTTCAGGTACTTTGTATAAAGTTTTTTGGTCTTCAGAAATGAAATATAAGTTAATCAATGGTGTATTTGGATTAGAAGAAATTAGTTTAGTATTATATTTTTTATTTTCTGAGTAAGATTCAATAAACTCATCACAACCTGGTTCACCTTTAATAGTACTAAATTTTTCAGGAGGTAATAATTCATTTTTAATATTATTTGAAAATATTTTTCTATTGAAAATGTCATCTAATTTATTATTCTTTAATAATGACTCAACTTCTTCGGCATGTTCTTTATTAGTTTTATCGATTGAAATATCAAAAATCTTAATTCTTGGAGAAATATCATATCTAATTTTAAAAGTATTGTTGGTATTTTTTAATTCAGCTTTATTTGATTCATTGCCAAAAACTGTCGAATTATTAAAATCAGAATTTTTTAATAGTTCACTACAATAAAATGGATAATCAATGTTTGTTTTTTTATTGTTTTCCATTTTAAATGTTTCTTCTTTAATAAAACCTTTAGAAATAAATAATTGTTTTAAATTAATTTCTCAAATGTCATTTTTTATTTTTTCAACTATTGATTTATCATTTGAACTTATATAAATGTTGTTTATTTTTAAAATAACCACATCATATTCTGAATTGCATTGACCTTTAACAAGCTCAATTCTAGGCATCTTTTCTCCAAAGTTTCTTAGTTTAGCTTTGAATCTAGTTTCAATTTCATTTTTTATTGTTGAAAAATTAATTTTTGACTCATCGCTTTCATTTTCTCAATCAATCATTCCGCTTCTAAAGTAAAAATGCAAGTCAAAGTTAACTTGTCTACTTTCTCTAGGCATAAAGTTCAACATAATAGTTTTATTACCTGCAAACTTATCGCTATAGGTAATAAATTTGTTGTTAATTTTTGCACCATATTCATCTATTCTTCAGTCTTCATTTTGGCAAATTTCATTAATAAATTTGTTGTCTTTAAGTAAAGACAATACTTGTTCTCAAGCGCTTCCTGATTTAACTTTTATTGAATCAATGAACACATCTTCATTATTTTTGTTTCTATATTTTAGAGTTAGTTTTGTTCAGCTATTTACATTGTAAAAAGCAGCAGCACTAACTACTCCAAGTGTTGTTAACGAGAGTGCAGTTGCCACTGTCGCTATAACTTTTTTTCTTTTGTTTTTAATCATAATTTCTCCTAAAAATAATTCATATATAAATTATATACTATAATTTTTTCGTTTTTATTAGATATTATTAAGTATTAAATTTGAAAAATATTTATATTTTTCTAATTCAATTGTTTTATAGTTGTTTTTATTTAAAAAATATTTAAAAATATATAGAAAAAGATTTTTTAATGATTTTGTATTAAAAAAACTAAAATTTGAAGCCATTTTTTTACTAAAAAATCTGTTTTATAGCCTTTTTGGTTAAAAATAAAAACAACCAAATATTTGTCGCAAAAATAAATAATTTATATTAATATATATTTTAATATAATATAATCATATTTAATATATTAAATGGAGGAATAAAACATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGAAACGAAGGTGAGGCTAAATATGATATTCGTGACTGAAGTGAAGATCACTTACGTTCAAGCAAGGGCATTACTCTTAGTTTAGAAGAATTAAAACAATTAAAAGTTTTGATTGACAAAGAGCTAGTAAGAATTAAATAATTATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAATAAAGGAGATAATATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGAGACTTAAAGAAAATTGCTAATCCAAGTGAAATATTCTTTGTAGCTGATGCTTTAAGTGGACAAGATATTTTAAATGTTGCTGAAACTTTTAATAATAAATTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAATGCATAAAACTGTTGACCATCACCACCCTAAAGGTTTCTTTTCCTATAGTGGAAATCATTTGAATTTCACAGGCTTTTCACAGGCATTCTTTTATATAATAGCTTTTTTGTCTATAACTTGTCTTTTACTAATTTGACTGTTTCATAGACAAATTAAACAAAAGTACTTAGTTAAAATTAAATTATTTTATTTACAAAAAAGAACATTTTGACTATTATTTGGCTTGATTATTTTATTAGGAATGGTAATTCATATTATTATATTAGCTGCTGATAAATTTCATCGAGCTTGAGAATATTTGCCTTTTCATTTTTGCCGAATTTTGATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGATATTATTGAGATTACTTGTTTGCACATGTTTTTGTATTATTGATGCCCGCAGTAATGTATGCTCTCAGCAATAACAAAATTAGATTTAAAGATTCAATTTTTACAGTTATTTTCTTTTCAACACTTTCATTAACAATGTTTTTAATTAACTGAATAACTTATACATATAGTAAAAAATTAACTTGAAAAACAATGAATTACTTTTATTTAGGGCCTAATGAATATAATGGTTTTAGAGACTTTATGGGACCGCTTTCAAAATGACCTTACAGTCTTTTTACTTATATATTTCTCGGCATTTTAGCAGTTATTATCTCAACAATTTTTTACTGTTTTCAAGACAAAATTCATTTGGCTAAAGTTGAAAATAAATGAGTTTTAAAATGAATCAAAAGTGAAAATTGAAAAATATATCGCTCTTCATTTAATACAAGAAACCAAAAAAATGATGAATTAAATTCTAGTTCTAAAGACTTAGAAAACTGTCAAGATAATAATGATTCTATTGAAAATAATAAAGAAAATATTAGTCAAACAGTTGACTAAAAATTAATTAAAAAAGAATCAATTTTGAGCTCAAAAAAATCTCTTAAAAAAATTGATTCTTAAAAAATGAATGTAATATAATACTATTTATGTTAGTAGTTTATGACATGCTTTTTGTGTACTTTTAAAGAAAAATACACTGGAGATCTTATGGATAACCAAAAAGAAAAAAGAGTGCATATAATCTTTTGAAGTTTAACTTTGATTTTAGGTTTATTATCACTTGCTTTTTTTAATTATAAAAAAACTAAATTTGCTGTAATGCTTGGCTATTGAATTGGCACAAGTTGTGGCTTTTTAGCATACGAACTAAGAGAGTTATTATCTTTTAAAATTATACAAACTAGTAAAAATAGAGCAATTATGCTTGGTTTCTTTGGAACAATATTAAATATGCTTTGAATTGGATTATTGCTTTTTGCAATTTTCAAAATTAATTCAAATGCGCTTAATAGTCCTTACACAAAAACCATTGAAAAAAATAGTTTTTGACCAATAAACATCATTGCTTTTATTCCTGGTTTTATGACATTCTATTTTTCTCTTTTCATTGGATATTTAAGTTCTGAAAAAGACAAAAAAGTTCAAAATAACTAGAAAAGGAGGTCAAGTGGAAAAACTGACACAAAACTTGTGAAAATGAAATATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGAATCTGAACACAAATTCCTGGCCTACTAGGTGAATGATTCTTTATTGGATCATTAATTACACCATTTTTACATATGTATTTTGATATGTTTGGTGCAATAATTCAAGCTTTAGTTTTCTGTACATTAACAACAGTTTACTGATCAAAAGAAGCTGTAACAAATGAAAACAAGAAACAACAAGATGCTCAAGAAAAAGCAGAATCTGAAAAATCAGTTGCTAATGTTGAAAACAAAGCTGTTGCTTGATCAAATTCAAGCAGCGTATATTAAAAATTAGGAGGTATTTATTATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAATTTAATAATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAATTTTTCGTATTTATATATAAAACAATTAATTTAGTAAAAATATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGAATGGCCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAGGAGGTAAAAATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAATAAAATTTGCTAAAACAACTGTTTCATAGTTATATAAGAAAAAATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAATTTAGAAATTAATGGACCATTTCACAAGGTTCATTATTTTTTATTTTTTTGTTTCTTAAAATATATTTTTATACTAAAAAAATCATTGCTTTAATGAATAAAGTGCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCATATTGAAAGCACAGGTTTGATACAATGAAAACAACGTATCAAGCCTTTTTTTGTTGCTTTTTATAAGCTTTGTACGGCCTTTTTATTAAAAATAGGTATCATTTATCCTGGTTTCCACTAAAAACAAAACAGTGGGCCAGGATTTTTTATTTATTTGACATTCTTACAAAAAAATGAAAGGCCGCTATAAAAAAACGATAGGTTCTTCATATTCGTTAAGTGTTTTTACAATTTTTTTACTTTTAATATAACTGTGAAACGATAGATTTTCATAATAAAAATTATTCGAGAAAAATATGGAAATATTCAGATATTGAAGAAAGCGTTCTACAATAGCGAGAAGATCCTCAGGGTGGTTTCCTTGGCTTCGTTGCTTATCGGATCAGTAACAGGTGTCGTTTGCTTTTACTTTATCCAGTCGATTATACCAGTTGCAAATGTTGCATTGGACATTGAAAGTTATAAAAATTATCAAAAATGTTGTTTTTGGGCAAAATAGTGATATGATAATAGCATAAAGAAGCTTACTTTTAGTATAAATTTTGTTGAAATTTTGACTTATTTTTAGTGCTGCTGTTATAAAAATCACTACCCAATATTTTAGCTTTTTTATTAAACTATTGAATTTTTTTACCTTTTATAAATACGGAGGAAGCCTTATGGCGATAGAAACTAAAGTAATTAACACAAGTATGTATGGAGTATTTACCCAACAAGAAATAATCGACCATTACACTTGTTTTGGATGACAAACTATAAGTGTTACCGATACCAGAGTAACTATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGAAAAGCAAGCATAATCAATGTTAAATTATTAATTATTTTGTTACTTTTATTCATTGTGGGTGGAGTATTTTACATACTTAACAAAATGAAATGAAGAAAAATTTATAGAAGAGAATCAAATGCTATGCTTGATGCAGTTGAAAAAGCTAGAACATTATATTATGCATATAATTAATGTTTAATTTATAGAACTACACAAGTAATACTGCTTTATGTGCTGCCTATAAGGCCGCACGTTTTTATTTAATGCTATAAGAAACGAGATGTAATTATATTATTTCCAACGATGCTAAGCAATATATATTTTGATAGTGGTATAGTCCTCATTGAACATATATTCATAGTTTGAATCTCTTAAAAATTCTTCAATATATGCTTTTATAGTTTCAGTTCCTTTGCCGATAATGGCTAATATGTAATTATTGCTAAATTCTGAATTATCTTTAGCATTAGCAATTGCAAATTGAATTTCTGCTACTGCTTCATAAATATTTAACCCATGTAAGTCGATGACATTTGATTCTGCTTTATCATAATTGATTATTTTTTTATAGTTTCTCTTATTTTTTCTACTCATAATTTTCTTTCTGGCAATTTGGACAATAATAAGTCACTCTTTGAGGTAATACCGCTTGTTCAATTTTCTCGCCACATACTTCACATGGTTTATTAGTTTTTAAATACACTTTTAAAAAGTTCTGGAATTGGCCTTCAACCGCATTCAGTGATGAATAATCGTTAATTGTAGAACCACCTAATTCAGTAGACTTATCAAGAATTTGCTTAGCATTATTTATTATTTCTTTTCACTTAAATAATGGATTTTATTCGCTTTTGTTCAAGGGCTAATTTTTGATTCAAAAAGAATTTCATTAGCGTGTATATTGCCTATTCCTAAAATGTAAGACTGATCTAATAAAAAAAATTTTATTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTCTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAACAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGAAACAGATGTTACCGAATTTAAATTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATAAATTTTAGGACCAGAGAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAGAAATGTTAGAAAATGTAGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCAAAACATAATTAAAAAGTCCAATATTTGGGGTTCATACCATTCATACCAAGGGTCTCACTTTTGAAATTAACAATTTATAGAATTAACATTTTTTTGTTTCTTAAAATATATTTTTATACTATAATATCAATACTTTAATGAATAAAGTGCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCATTTGATTAAATTCACCGTTTCTTCCTTATTGGAAGATTTTTTATTATAAAAAATAGCCTAAGCTAGGCTACTTATAAATTTTTAATCAACCATTTTGAATCAAGGAACATTTACTTCTTTGATTTCTAAAGGACAAAGCAATTTTGTTTGTTTGTTTTTATTAACATTATCTGTAATTAAAACAATAACATCACTACAAATTTTACTATTTCTTAATTTCTTAAGATCAACAGTTGCTAAGAGTTGTCCTCTTTCAACTTTATCATTTTGTTTAAGATTTGTTTCAAAGCCTAAACCATTTAATTTAACTGTATCAATACCAATATGTACTAAAACTTCAAGACCATTATCATTTGCTATACCATATGCATGTTTAGTTGGATATGACATAATTAATTTTCCATTAATTGGTGCATATACTTCTAATTTGTTTAATTTTTTATCAATTTCAATTGCATAACCACAACCTAGCAAACTAAAGGTTTGATCACTTAAATCTTCAATTGATTTTCTTGTACCTTTGCAAGGTGTATAAACTATATTTTCATCAACAGTTTTTTTAACTTCAACCGGTTTTTCTTCAAATAAGTTGACATCACTTTTAACTTCAATTTTTTTATTTTTAAGTGCTTCTTCTTCTAAGAATGGTGCAGCTTCTTGAATATCTAAATTTAATTTTTCACTAATTTCACCAAATTTAGCTTGCACATGTCTTTTTCCAATAAAAATAACTTCACTTGCCCCAACTTTTTTAAGAGCTTCTTCATTTACTAATGAAGTATCTTTAACATCATATCTAAGACGAACAGCACAGTTATTAAAACCTGTAATATTTTCAATTCCACCCATAGCATTAACTATGTCTCTTGTAAGTTGTGAAATTTCAACAAGCTTCCTAACTTCCTTAATTTTTTGTTTATTGTCTTTACGACCAAAAACTCTTTTAATTCAACTAACAAATCCCATTTTGAATTATCTCCTTGAAAATTAAATCTTATACGTAAGATATACTTAATTAATATTAATATAAAAGTTATCTTTTGAGATTATCTTTAGATTAATAAATTAATATTATATGAAAACATTAACATTAATAACAATAATAATTTGAAAAACATGAATTTAAAAATTAAGTTGTTTCATATAATTAATAAATAAATTGATTAAGTTATATTAATTTAATTAATATGTTTTGTTAGGAGAAAAAATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGCCGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGATTACAAAGATCAATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGAGAAGAATTAATTAGCAATGAAAATGTTATTATTACTTCTCACCAAGCATTCTTAACAGATGTTGCTTTAACTCAAATTGCTAAAATCACTTTAGAAAATGCTGATATGGCACAAAATAAGGACTTTTCTAAAGCATTAGTAATAATGGAAAATGGAAAAGTTAAAAACGGATAGAAAATAATCCTAATAAATAAAATAAAATTTGTATAAGCCTGTTTTATAGGCTTTTTTGTAAAACTTATTTATTGAAAAAGGAGAATATATGTTTAATTATTTTAATTTAAAAAATCGTCAGGCTGCAGTGCAGCCTACTAAAGCGATTCACTGATTTAGTCACTTATTTAGTGAACTAATTGGAACGATTTGAATTTCACTTGGTTTAGCAGGTTTATCAATTATTATTAAAGATAAAGTTGTTGAACATTACTTATTACACAATGTTATTGTTGGCTTTTTTGCTGGCTTTGTAGTTGTCGGATCATGCTTAATTTTCTTTGGCAGATGATCAGTTGATTTAAACCCAGTAGTTACACTTTATCGTTGATTTAATGGTACTAATAAAACTGGTTATGTTATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGAAGTGGTGGTGTTTGAATCTTCTTTGGTGAAATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGAATGGGTATTCTGCTAGGTTCAGCTGCCATTAACCCTGTTCGTGGTTTAGCTCAACAAGTACCTACCTTATTCTTTGATATGAAACACTTAAGTGCTATGGCTCAATATGACATTCACATGGGTACTTGAGCAATGGTTTTAGGTAACTTAATGTCACCAGTCTTCCATGCATTCATGCAAGGATTCACTGAAAAATATGGTAATCCAGCTTTACTTTGAATGTTAAATTACAAAAATAATAAAGCTAGTCATTATGTAAAAGAAACACAAACAAAAGAAGAAAGCGTTAAAAAAAACAATTAATTTCAAGATAAACTTAACAACCTAGTTATGTTTATTTTTTATTATTTATGCTTCTATATAATGAAAAACAAACAACAATATACCTTCTTTATTTATATACAATAGAGCCTGCATCTGTGTGAAAAATTTTTCAAAAAGTTTTTGCATTCGAAAAAAAGCGTATATAATTATTGCGTTATGTTAAAAAATTGAAACTTTATTCGTATTGAAAAGAATATCAACATTATCAGAACAATTATGATGATGTCTAATTTCTAATTCAATACCTCTTAAAGATCAAAATGTAATATAAATTTTGTAGTTTTTAAGCAGGTATGCAACAGAAACCTGCTTTTATAATAGACGCTATAGAGGAGCTCTTAGTAATAGTATATTAGTTTAGCAGGCATGTGTTGAGACTATATAAAAAGGATATAAGAGCCGAACTGGTGATTGTGGCGGCAATTACTGGTGGTAACAATGCATAACAGTTTGTTACTCTTTGGACCTCAAACCATTGGTGCTATAGCTTTGCAAAAATAAATAATTTCTTTAATTATTATTTTGTAGAGTATTTAGTGCTTTACAAAATTTTTATTTAATCATAACATTAATTATTATTAAAAAAGATATTAATATATCGAAAGGAAATTATGAATAAAAAAAGAAGATTATTATGATCTTTTGCCCCATTAGCTACATTAGCTGCTTTTGCGCCTGTTGCAATCAGTGCTAGTTGTGGAAGAAAAGGCCGTGTAATACCTAATCTTGACTTAATTAAAGGGCAAACTAACTGAAGTGTTGTAAACTCTAAATATACTGAAGATTCATACACTACAGACCTTTCAACAACTTATGGTGGTTTCTTAAATAGATTAAAAGAAGAAACAGGAGCTTATTTACTTACAACAAGAAGTTTCGGAAATTGCAAAATTGTTCAAGCTGGTCCAAAAGGGCATGAGCGTAAATTTGTAAAAAGTCCTTCATATTGAAGATACTCATTAGAATATGCAGATGCTGTTGTTGTTACAAAAGCAGATGGTACAGAAGCTGTATTTGACTCAGATGAAGCTGAATTAAAACAAACTCCAGAACATCCTGAAGATAAAGCAAAAAATATACCTGCACACTTTGATTTAGCTAACTATCATGCTAGATCAGCAAATGCTAGAAGTGTTAATAGTGCTGAATTTGAAAAAGCTCTTAAAGGAGCTAAAAAAATCCAATTGCGTGTAAGAAAAGGTGCTAAATGAGTTGATTCATTAGGCAATGAAACAAAATATGATGTTGTTGGTGATGACTTCTACATCTCATGACTTAGAACTTACACACTTTCAACTGGTGTACGTAATAGTTACTTAAAAGGTACAAAATATGAAGACAAAGTAGCAGAACTTGATAAACTTGCAAGCGGAATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGAAATGGTAAAAATGGAAGTAAAAGTAAATCAAACTTATTAAGTGCTTTATCTGAGCTTCCAGAAGATAACTTATTAAGAGTTGCTGGTGTATATACTTATGGTGCTAGTGAAAAAGACATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTCCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGAAATGCTGTTGCTAACACATTATCAAACAATAAAACTAAACCTTGACTTGCTGGTGTTGCGCCTCATGGTAAAATTGGCGGTTCAGATCAAGATACAACAGCTGCAGCGAAAAGACCTGTTGACGATTATGAAAACTTAAACACATTCTACACAGTTGATTCTAATGGTGAAATTGGTATAACAGTTACACCTACAGAAAATGCTACAATTGCAAACACTGCAAGTACAACTGAAGAAAAATACAAATCAGCTAAATTTAATGAAGTTAAAGCTGCTATTAAAAAAATTCTAGATGATTATGAAGCTACATTGCCAGCTGGTGAAAAATTAGAAGTTGAATGAACTGTTCCAAATAGATATATAAACTGAAATCCAAGTAACATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGAGGTGATTCATTATTTGGTGGATCAATTGCCAAAATTATTAGTTGAGGTTATGATATCGATAACATCGGTTCAGGTATTGATGGTATTACAAACAGTGGATTTATCGGAGCATTATTCTCAATTGGTACAAGTGCAAAATTACAAAACAAATTACAAAAAGCTTTTCCAACATTAGTTCAAGCTTCAAAAGAACTTGTTGAATTTGCTTTAAAATCAGAAAATAATTTTAAATTTGGTTTTAACTTACATGACTTCAATAAAATACCAACAAAATTTTCAAATGATTTAGCTGAAAACCTTCACCACTTAAAATGAGATTCTAAAGAAAACAAATTTGTTTTTGACGAAAAAGAAAACTTCACAATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTGACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACATTAATTAATAGCGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGATTAGCAGATATAATTGTTAAAAATGTAAAATAATAGTGTAAATATATATTTACAAAAAAACTATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGAACTATTTTAATTTCATTACCAGCTTTCGTTATTAGTGCCATTTTAGGTGTGGCAATTGGCGTTTTAGCTGGATATAAAAGAGGAACAATTTGAGATACGTTGATAAACATTTTCATTTTTATCTTCGTGGCTGTTCCTTCATTTGTTTTAGCTCCATTCTTCCTTAATATCTTTAATAAATTAGGATTTGGCCGAACATTTGTTTCACCATATGAAACAGATAGTTCATGAGCTAAAACAATTAAATCAATTATTCCTCCAATTACATTATTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTTTATTAAGTGGAAGTATTATTATTGAAAGATTCTGATTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGATTTGAAGTTATTAGAGCTTCAAGATTCCGTAGAAAAGCAGAAAAAGAACTATTCAAGCAACAAGATCAAGAATTATTAATGAAAGGAGCGAACTAAAACATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGAGAAGGCTATCATTATATTGAGCCTTACTTAAAAGATGTCATAGTTAATAGTAAAGAAAAATTACTTATATATAATGCCTATGATTTTTGAAAAGCTTATTATATTTGATCACGTGCTTTAAAAATTAATCCTTTAAGACCAGAAGATATAGAAAACATCAAAGCTTCAATGCCACATTTAAATACTCTTTTTGGTACAACAGTTAAAGAAGCAGATATTTGAACAGGCGCTTGATTTGGTGCAATTAGCTCAATTAAAGTTGCTTTAATTGTTGCAACTATTGAGGTTGTAATTGGTGTTGCTGTTGGTGCTATTCTTGGTTTCCATGCTGGAAAATGACTTGACACTGTAATGATGAGGGTTATTGAAATCTTCCAAGCACCGCCTTCAGTTATTTGATTATTACTATTCATTTCTGCTGCTGGAACTAGTGAATGAGTTTTGATTGCTGGATTACTTTTTGTTGGTTGAACTTGACCTATCGGTGCAACTAGAATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGATTAATGTGACTTCCAACTCTTATATTATTGACACTTTCAGTTAGCTTACAATTTATTGCTATTGGTCTTCACGATGCTTTAGACCCAAGAGTTATTAAAGTAAAAGGATAAAATTATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAACTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATACTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGACAATCAATTAGAGGAACATATATTGGATATATCCCCCAAGATCCTCTCACAAGCTTAAACCCTACTAGAAAAATAGGTCTTCAATTATTGGATGCACTTAATCTAAATAAAGAATGAAAAGGCAAGAGCTACAAAGACAAAAGAGCCTATTTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCATCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGAGCTTTAATTTCTGCTATTCCAGAAAACTCACAATCAAGACTTTACTCAATCAAAGGAACACCACCTGATATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGATTATTACATCCAGATAGTCCAAAAGTAGAAAGACCAGAAGAACTTGAATATAGACTAAAAAGCTTTAGAAAGGTATTTAAAGATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGATTTGATATTAAAGAAAACTTCTTTTATGTATTTAAACAAAGAGCTTTAGAATTAAAGCTAAAAAATCTAGAATCAATTAATCTTTTAGCTGAAAAATTCTTTCCATATTGAATTGAAAAATTCAAGAAGATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCATAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGAGTTAAACCATTTAATATCAACAGTTTTTCATTAGCAACAAACAAATCAATTGATGAAGAATACAATTCAAAAATAGATCATAAACCATACGATGGACATAGCGAGATCCCAAATAATATCGAATATACAGATGAAATTAAAATAGACGAACCTACATGTGAAATAAAAATATCTTCAAATTTTGACTTCTCCAAACCTATAGTTGAAGTTGTAGAAACTAAAGTTTCAACAACCAAAACAACTAAAAAAAGAAGTACTTCTAGAAAAGCTTCAAGTGCTAAAAAAACAACAAAGAAATAATTTTTGTTAAAATTTCATTACTATGAATTCAACAAGAGAATATTTTAGAGAAGCTTTTACTTGAAAAAAACTTCTGCACTTATTTATTATTTTATTAATATCTTTAATAGCAGGTGTTTCACTTTATTTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAAAAATCTAAAATGCACTAAGTGTGCATTTTTTGTTTATAAATAAAAAACAAAAATATAATATAATTAAAATAACTTTTTGAAAGGAGATAAAATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGAAGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGAAAAATATTTAATCAAGCTGCAACAAATGCCAAGGAAAAAGTATGAAGAATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAATATATAGAAGACTTTTTCTTAATTGAATTAGTCTTTTTTATATGCTTTTGTCTAAAATTTCTATTGAAAAAAAGTGGTCAAATGACCAGAAATTTTCAATAGAAATGGTTAAAAATAGCAAAAAGCGAAAATTCTACTGAAAAAAAATGGTCAAATGACCAGAAATTTTCAATAGAAAATAATAATTTAGTATAATTTACATGAGGTTTTTATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACCGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGAAAAGAATATTGTGAATTTGGTGGTATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGACTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGCTGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGGCTTTTTATTAAATGATAACATTTTTTAAAAAAGCTTATAAAACAATTTAATTAGTCAAAATTAAAATTGCAGATTTTACTGTCTGCAATTTTAAATTCTTGTTTTTAATGATAGTTCACTTTTACACCTGTAGGAAATAACTTTAGTAATGAAACCAGCTTTGTTTATTTCGATTTCTCATGATTTAGACAATGTTATTTCTAATAAAGCTTTAGTTCCTCTTTATATTGTGTGATTGTAAGTTTTGATGTTTTTAGATACAACTATTTTTTGATTTTGGTATTGTTAACACTATCAGGAATAATAATTTCATCGATTAGTTGTTTGCTTTCAAAAATTGAGAGTTAATTTTTTGCACACCATTTGCAATATTAATTTTTTTTACATTATTGCAACCACGAAAATCATACTTTTCAATAATTTTAACTATGCTCGGAATATTAATTTCAGTTATTGAATCTAAGCTCTTAAAGTTGCCTTGTTTAATTGTAATTAAATTATTATTATAATTTTTAAACTAGTAAATTTTGTATTTAAAAATGTTCATTCTCCAAGATCTTTTAAATAAGTTGGTAATACTAAATCGCCTTTTAATTTAGTATTTCTAAATGCTTGTTTGCCTATAGTTTCTATTGTATTAACAAAATCCAATTAAACTACCTAAAAATTGTGTTCCTAAAAGATATTGAACACCTTTTCACACCATTGTTACTTGCATATTTTCAATATTTGTCCATTGCAAAACAATATGTAAGAAAACAAACACAAGGTTCATTAAATGAATAACAAGATCTTTTGTATATAGTTTGCCAATTCTCACATTGTAAAAGCTATAATAAATGTTATTGCAGCAAATCAAATAGCCAATGTTTTCATAACTTTTTTAATGTTTTTAATTCTAACGTATAGCATTCATACAAAAAACTATAAAAAAGACTGATAATAGTTCATTTGTAAATGCAACTTTATCAAGAATAAGTATTAAACATAGCTTTCATTTCTGCTTATCTTTTCCTTTCTAAAAATAAAATTACTTATTGATTATTTTAATATAAAAAATTTTTATTCATATAAACACTTAAAAGGTATCACATATATGTTGTCTTTTGTAACATATGATTTATCACCTGTTCCACTGATAACTATTAATCATTTAGGAACTTTTTTATATTGTTTTTCACTTTCGTTTTTAGCTAAAGAAGCTTCTTTAATCATACTATCTCTAAATTTTCTTAAGTTTTCTATAGCACCAGCTTCTTGCATTGAGCCAAGTTTGATTTCAATAGCAGCATAATCACCTTGTTCAGTTTTCAAAATGCAATCTACTTCTTGGCCTCATTTGTTACGATAAAAAAGGACTTCGACATCAATTAATTGACTATAAATTCTTAAGTCTCTTATTACTAAAGATTCAAAATAAACACCTAGTGTATTTTTTGTTTCATTTAATAGTTGAGGACTTAATTTTAATATTCCTGCAGGAATTGAAGGATCAATAAATCTTATTTTTTCAGAATTTAAAACATAATTACTACTTAAAAAGTTAGGTTTAAAGTAGGTTTGATTTTCTATTAAATATAAACTTTCTGCAGTTTTAAAATAATTATTAATTGTTTTAATGTTGATATTTTCATTTTCATATTCCATAATGTCTTTAGCAATAGTTGTTTTTTTGCAAGTTTGGCCCTCATTACGAGCCAAAGATTTTAGAAACATAGAATATTTATTGTAATTTATACGATTATCTTTAAAGTGACTTATGTTTCAAGTCATTAATGAATCAACATAATTTTTAATTCAAATTTTGGCTAATTCATCACTTGATTTATAGTTATTAGGTCATCCGCCTTTGATTATAATTTGAGCTAATTCAAAAGTATCAAAAGGTTTTTTAACTTTAACTTGTTCAAATTTATTTTCATTGATTAATTTACTTAATGAAATTTGTTTTGAAGAAATATTCATTTCACTTAAACTCATTGGATACATTTTTAATCTACCAATTCTTCCAGAACCTGAGTGTTTAGGTTTTTTGACAACAGGTTGAAAAGAACCAGTTAAAATAAATTGACCTGGTTTACCTGTTTCATTAATTTTGGTAATCACTCTTTCTCATGAATATGGATATTCTTGTCACTCATCAATTAATCTTGGACTTTGACCTTTTGTTATTTCTTCAGAATCAAAAAATTGATTATTTTCTATTAATTCTTTTGTTCCTTGTAAACTAATTGAACTTTTTGCCAAATGACTTGATGTTCATGTTTTGCCACAATATCTAGGACCATAAACTAAAACAGCTCCCATAACTTTTAATTGTTTTTCAAGTAAAGAGTCAACTATTCTTGTTTGATAATTATTTTTATCATAATTAATTTTTGCAGTCATTTTTTCTCCTTTTTATATAAAAAATTATAAATTAAAATTTTAATTTTTGGAAAAAATGGTATTTTAGCCTTGGAAAAACATGTATTTTGCTTACGGAAAAAATGGTATTTTGAAAATAATAAAAAACAAAAAGCACTATAAAATAGTACTTTAAGTAAAATGTATGTGATTAAAAATTATTGTATTTTTTAAACACACTGTAAACTGCATCAACTGCTAAATTGAATGCTGTTTTGTGACTAAAATTATCAATATCAGGATTCTTTTCTGATTTCTTTAAAAACAATTCTTTTGTTTCTTTTCAAATTCTGTCATATTTTTTAATTTCAGTAACTTTATCACCAGTATATTCATAGTCATAAACCTTTGCTCAATTGTAAACTATTTTATTAGCTTTATCTTTAGATCTAACTAGAACAAATCTTTCATTATTTTTATTTTCGCTGTATAAAGCAATATCAATTGAAAAATTTTTGAAATCAGTAAATTTTAAATTGAAACTTTTTGAATTTTCAACAATTTTTGTACTAAAACCTAATGGACAAATATGTTTTTGTAGCTTTTCAAGTACATATTTGTTGAAATCAACACTTTCTAAATTAATAGTTTTTTTAGGTTTTAAAATAATGTTGAAGTCATATGCAAGTGTGTCATCAACAATAGCTAACATTTTTTGACCAAATGGAAAAGTTTTTTCAACATAAAAACTAATTTCGCTTTTTTGATTGCACATTTCTTCACAAATATCGTCTAATTTATGTGCAACTCTCGCAATTAAATCTTTATTAATATTGTTTGGAAATTTTACTTTAGTTAAATTTAAATCTGAAAAATAAACTGCTTTAGCCATAATCTTCCTCTTTTCTAATTTCTTTTCTAATATCAATTATAAATGAAGAAAAAACAAATTATTTAATTTTGCAAGAATATCTATAAAATAATCATATTAGTAAAAATTTAGCTCTTAAAAAAACCTTAAAAAAATGTTAATTTCAAAAGTGAGACTTCTAGGTTTTAGAGATTTCACTTTTTAGATTGTCCTTTATAATATAAATTACTTAAAAAAATAAAGAAAGGACACTTAAATTATAATTTACGGGTGCAAATAAAAATGGTTATTGAAAGGCATTAATAAAATAATAGTTTAAAATATATTATTAATAAAGGAAAAAATGAGTTATTTACAAAAGTTATTAGATAAGTATTGTCCAAATGGATATGAGTGAGTAAAATTAGAAAATATTGCAACTTTTATTAATGGTTTAAAATGCAAAACCAAAAAGGATTTTTTAGATGGCAATGAACATTATGTTAGCTATTTAAATGTTTTTAATAATCAAGAAATAGATTTTTTGCCTACTTCAAAAGTAAAAATATCAAACAATGAAAATCAAAATTGCTTGCAAATTGGTGACGTAATTTTCTCGGGTTCTTCAGAAAATTTTGAAGAAACAGGTTATGCTTCAGTTTTTAATTTAATTTCAGAAAACAAAATTTATTTAAATAGTTTTTGCTTTGCAATAAGATTTAACAACAAAAATTTATTTTTGCCAAAGTTTTCAAAATACTTGTTTAATTCTGAAATATTTAGAAATCAATTAGTAAAGTGTATTAATGGAGTTACAAGATTTAACTTATCAAAAGTAAAATTTGCCTCAATTAAAGTTCCTTTAATTCCTCTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATCCTAGAAGCGGAGCTGAAAGCGGAGCTGAAAGCGGAGTTGGAAGCGAGGGGGAAGCAATTTAATTTTTGATTAAAAAAAATATATGGGAATATAGATTCAAAATATATTACTAAATTGGAAAACCTAGATATTAATATTGAAACTGGAAAATTAAATGCAAATAAAAAAAATGAAAATGGAAAGTATTTATTTTTTACATGTGATGAAAAACCTTATCGTATTAATGAATATGCATTTGATGCAGAATCAATATTAATATCTGGTAATGGTTCTAAATTAGGACATATTAGTTATTATGAAGGAAAATTTAATGCTTATCAAAGGACTTATGTTTTAACTTCTAAAGATGTAAATATAAACCTAAAGTATTTATATTATTTCTTAAAACATAATTTTAAAGATTACATTTCTTCAATTCATTTTGGATCTTCATCTGTTCCATATATTACACTTCCTATATTACAAGAATATAAATTAAAATTACCCCCTCTTGAAATCCAAAACAAGATAGTTTCAATTCTTGACAAATTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAAAATAAAATTTATTTGAAAATAAATGTTTTTTATATAATTATTTAGAATAAAAGGACTTATTATGAGAGAATTGATAACTTTAAAAAAGAGTAGAAAAACGCTAACCAATAGGTTAGCTTTTGCTTTCTTTCTTTCTCTCTCTCTCTCTCTAATCTATTAAAAAATATTATTTTTAATTAGTATCCAGCACACACTGGATGTTTTTTAGTTACCTTTTATTCAATTAATTTGTATATAAAAGGAGAAAAATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAACTTAACCTCACTCGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAGGCTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGAGCTGCATTTAAAGGATGTACATCATTAACAGAAATTAATCTTCCTGAAAATGTAAAATTCATTGGTAAACATGCATTTTTAAATTGTTCAAATCTTGCAAAAGTTGAAATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAACAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGAGATAATAAAGATATTAAATCTAAAGCTTTCTATGGTTGTGATGTGTTAACTACTGTAACATTTGCTTCAAAAGTTAAAAATATTTTAAAAGAAGCGTTCTTTGCTTGTCCTCTTTTAAAAGAAGTTTCTATACATAAAGGAACAGACTGAAAAACAGGACTTATGAACAAAAACAAAGCTTTTGATGAAACTATTACAACTGTTAAAGTAATTAATTAATAATTTTAGGACTTGCAAAATTTGCAAGTTCTTTTTTATGCTTATGTTTTCATAATTTTTAATTATGTGAAAAATTATTGTGCTTAAAAATTAAATTAAATATTAAAATATAAATAATTAAAAATTTAAGGAGAAATATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGAGACTGAGCAAGTAAAAACGACGTAACTATTTTTATTACAGCTGATCATGGTAATGCAGAAATTACTGAAGATGAAAATGGTAAACCAGCTACAAAACATACAAGTAGTCCAGTTATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAAAACAAAAATTACGATTCTTTAAAAACATAAATATCATAAATTTGAAATGCTCTTTTTTTATAATGAGCATTTTTTATTTAAATTTAATTATTAAAGTTTTTTAAAAAAATAAATTAACTTTATAATTGAAATATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGAAATAAAAGTGGTGAAATTAACCAAGATCTCCATAAAATAGAGCTTTTTGGAACAAGCGATGAAATAAAAAAAGCTTATGAAGAACTTAAAAAATTAAAGTTAAAACTCAAAGTTATTTACTTTGGAACACACATCGAAATTACTCCAAAAGGTGTTGATAAAGGCTATGGTTTAAAATGAATTTGTGAAAACATTTTTAATTGCAATTTTGATTATGTTATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAGAAATAGGCTATATTCAGCTTATTTTTTTAATATTAATATTAGATTGTAAAAAAATAAATTAAATAAGTAATCAAAATTGAGTTTTAATATATAATTTTGTTAAGCAAATTATATAATTTGTCTTATTTTAATTAAAATAAAAATTTAAGAAAGGTAATAGGTTACTTATGAATTATTACAACGATTTTGATATGAATCAACAACCTAAAGCGAAATTCTGAAGACCTAACGAATGAAATGCATCATTGGTGCAACCTAAGAAATGATGATACTTTGGCGTTATTGGACCAATTATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGATTAGGTAAAAGTCAACAAGCATTTAAAGCTGCTAAAGTTTTCTTTATAATGACACTTATATTGTTCCCATTCTTGCTTATTTGATGTTCAGTTTTAAACTCAAGAATGGATCCACCAGAACCTGAAGAAGTATTTAATCCTTATTCAGCTGCTAATGGTTATGGATTTAATCCAGCATGAGCCTCACAAACTCGTACATTTGCCTTAACAGGCCAATATACAGGTTCTTATGCTGCTCAACCACAACCTCAACAAACACAACAATTTGGTCCACAACCAAATCCACAAGCAACTGGTATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGAATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAAAAATTTAAACAATATTAAAAAAACTTGCAGACGTGCAGGTTTTTTTAATTTGCCTATAAAATAGAGCTTTTAGAAAAACAAGCTTAATTAATTTTCACTATATAAAAAGTTAATAAACATTATTAATATTGATTTTTAAATATTAGTATAAAATTAAAGTATTATGGGTTTTAAATTTAAATCAACAGAAATGCGCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGTTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGAAAAGCTGTTACAGAAAAAATTAAAGAGTTATATGAAATAGGACAACCTGTTTTAGTTGGTACTGCTCAAATTGAAGATAGTGAAATCTTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGAAGAGAACAAGCTATTCGAAATAGCAATGATGATACTGATTATATCAATTGATATGAAAAAGATTTAGTGCTTGAAATTGTTGATATTTATTGACAAAACCATATTGACACAATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAATGTATAATAATCATGAGCCGACATTGAGGTTAAAACTTTAATGGTGAAGTCCTAAAAAGGACTTTTTTTTTATTGTTATTTAGACCAAAAATGCCAAAAATTGGCTAAAAATAAGCTTTTTATAACTTTTTTCAAGAATAGATAATGTTATAAAGTATAATAAAAATATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGACAAGTTCTTTTAAGTGTTTTAATTGGCTTAATTTTTGGTTTAGGACAATTCCTTTTAGTCCAAATAACAGGACTTTATAACTTAGGTTTAGATGCTTTCTGTCAATCAGTTGCTCGGATTGCAAAACACTTTATTACTGATGATAACTGAAAAAATAATATATATAACCTTTTATTCTGAACAGTTAACTTATTAGCTAATATTCCTTTATTTATATTAGGTTATAAAAAAGTTAATAAGCGTTTTGGGTTTTTAACAATAATTTTTATGACTACTGCTACTATTTTTGGGTTGATAATTGGTTTCATACCAGGTAGTGAAAATTGATTTATTTTTACAAAAATTAAAGCAGAAGAATTAACATGAGATGAGGCAAAAGGCGGCAATTTAACAATCTTTGTTTATGCATTAGTTTGAGCATTTTTACAAGCAGGAATAGCAGCGGCTTTATTAATAATTAACTCAAGTACAGGCGGAAGTGATGTCTTAGGAGTTTATTGATCACACAAAAAATTTAAAGATGTTGGTGCAAGTTTCATTTTTGTTCACTTAGCATTTTTAACAGTTGCTAATTTAATAGGAACTTATATTCCTTTAATTACGCCAAAAGGATCTTTTAGAGAAGGCGCTTATAAACCAGAATATTTCTTTAATCCTTCATTTATTGCTGGTATTGTTATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAGTTGTCTTTTATTTTTTAAATTTTAGTATTCCAATTATTTTTTTAAATTATAATTTTTTATTATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGAACAAGTTTAGTATGAAAAGATAGTGACAAACTGATTGAGTCCTTCTGAGTTTTAGTGATTTATAATGTGTTATTCTGGCTGTTAATATTAGTAGCTAATATTCCTTTATTTGTTTTAGCATATAAAAAAATTAGCAAAAAATTTGGTTTCTTATCTATTATTTTCTTAGTTGTAGCTTGTATTACAGGTTTTTGTATTGGTTTAATTCCAACCTCTGAGCAATGATTTATGATTTCAAAAATTGATTCAGCTAATTTAACTTGAGAAACAGGTAAATTTAAAGATTTTTCAATTTTCTTATATGCAATACTATGAGCATTTTTACAAGGAGTATTTGCTTCAGCTTTATTAATTATTGGTTGTTGTACAGGAGGAGCTGATATTCTAGGAGTTTATTGATCACACAAAAAATTCAAGGATATTGGTGCAAGTTTTATACTTGTTCACTTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAAAAATCGGGAACCCCGATTTTTATTATTAAATTTTATTATTTTTTGCCTTTGTGGCTAATAGCATCTCTAACACGGCCTGTTGTTTTGTGAATCAATACAGAACCACCACGTTTTTTAGCAATTTCATTTGCTAATTCAACAGCTTCTTTTTGAGTTTTAACAATTTTTGTAGGTCTTGAGTTCCCAACACCTTTAACTTGTCAACCTAAGCCTTTAGGAGTTACGTGGTATACTGTTGCTTCTTTGTTTTTTGTTTCTGCCATAATTTTCCTTTCATTGTTTTTATTAAGTGTATTAATATTATAAAGAAAAAAACAACAAATTTAAAATATTTTTTTAAAGAGCATTTTTTCCAATAAGCCTATTTTAAAAAGCTATAAGTCACAATTTATTTTTGAACATAAAACCTAATTTTTTAAACTAGTCTTCAATTTTAGCTAAGAAGTCTTTAGATTTTACATTATTTTCTTTAATTGAAAATATAATGTTGACTTTGCCATTTTTATCAAGAACTGTATATAAAAATCCATGAGAACCTGCATTTTTACCTGTAATTTGTTTTGAAGGATGTGTTATAACTTGAGATGGTTTTTTCAATCCAGTTTTTTTGTAAGCTTCAATTGCTTCTTTTGTTAATTTAAGCGAAGTTAGATTAATTGGTTTTTCCTTTTTATTATTACCTAATTTTTTTAAAGTAAAAGTATAATTTTTTGGATTAATATCAATTCTCATGTTTTTGTTGTTCATGTATTCTTTAATTTTAGCTAATTGAGTAGCATATCCAGGATCAGTTTTGTCATAGGTGAATATTTTGCCTTCTTCTGCCAGCTTTCTTAAATCTTCATAAGGATTTGCTGGAACTTTACCTTGTAATTCAACATGAGTAAAGTATTCTTTATCAATGTTTGTATTTTTGTAAGTTACTTTAACTCTTAATTTTCCTGCTTTTTGTAAAGCAAGTTTTTCGGCATTATTTTTTGGTCAATCAACAGTTACGCCAGTTAATGTGAAAATGTCATTTGTTGTTGTAGGTACATTTAATTTAGTTTTTTCATAGATGATGTCATTATCTGTAGCCTTTGTAACATCACAAGGGTTAGGTAATGAAAGATTAATTTCAGCTAAAAATGTATCTTTTAATTCTACAAATGATTTTTCTGCAAAAGTTAATTCAAATTTCTTTTCTTTTGGAGTTGATTTAGTTGTGTATGAAACCGTAATAGTTAATTTACGTGTTGCATCATCAAAATTTAATGTCATTAAATTATTTAAATTAATTGTTGTGGCTTGAATATTCAATTTTTCAAGATTTTCTTTATTGAATTCTTTTTCTTCATTTGTATCTGGATCAGTGTAGTAAAGTTTTACTAAATTTTGTTCTTCTTTAATTTCTGAATAATAGTTCTTTTCACTAATTTTAGTTTTTAAAGTACCGATTAAGTTTTCAAATTTGTCTTCTGAATATGGAGCTGGTTTTTTGAAATCATTAATTTCATATTCAACTTCAACATTTTCTTGACTTTTATCTTCAAAACTTAAAGTTAATTTCAATTTTGCTTGACCTAAATTATTATTAGCAACAATTAATTCTGTTTTATTAATTGTAATCTTTTCTTCACTTAGATATTCGCTTAAGGTTTTACCACTTGGAGTTGTGGCTGTAACTTGAGCCAATAAATCACTTGTGTGAGCTGCAATATATTCTTCAATTGTTTTACTTGCTTTTTCATTTAAGTCAACATTTGTTAATTTAGCCATTTTTTGTTGAATGGTTTCTTTTGTAACAAAACCAGTTATTTCTTTGTTGTATGCATATTCTGGTTGATTTCATTGTTGTCATTTTGCAACTAATTTAACTTTTCCTTCAAAATTGTCAACAACTTCTAATTGATATTTAATGTAATTTAAACCTTCCATGTTTTTAAATACAGCTAAATCATTTTGATAATCTGCATTTGTGAATGTAATTTTTTCTTCTTTTTCTTGATCAGTTAGTGCATTAAATTCTTCAACATATTCTTGTGCAGTTTTAGTTGATTTATCTTTTAAATCCACATTAAATGCTGCTTCTAATTCACTATTAACTTTAATTTCTTTAAAGCCTGCAACTACTTGACTTCAAACATATGTTTCAGTTTTAGTGGCGTCTTTTCAACCAAAAGTAATATTAATTGATAATCATCCTGAATAAGGTTCAGTTTCACCTGATGAGGTATCAGCTTCTTTATTTTTAATTTCAATACCTTTTTCATTAAATACTTTTGTTACATCAACTTGTGTTTTATTTTTGAAGTATCTTAGATCTGTAATTGTATAGTTGAAAACATTGAAATCTTTTTTGTTTTCTAAAACATCAATAGTATCAAAAATACCTTCTAATGTTGCTTGTAACGATTCAGTTTCTTTTTTGAATCCAGTTAATTTGATTGTAATTTCTTTTGATTTAGTTTTTGCTTTATTTTCAAAAGTTGCTTTAATATTTGCTTCACCTTTTGAAGCACTTACTTCTTCTATTTCTATGCTTTTAATTGATATTTCATATTCTTTTAAGAAAGTAGCTAAATCTTTGCTATTTGAAGTTGCTATTGACAATTTAGCTTGTAATTTAGCTAGATCATTAAATTGATTTTTGTATTCTTTAACTGTGTATTTGTCTTTTTCTGCTAAGTCCAAACCAGTTATAAATTTGATGTTTTCGTCAAATTTGTCAGTATCTACATAAAATCCTACAACCTTAATTGTGAATTTTTTAATTTCTTTATTTCCTTCTTTTTGAGTAAGGAAAATTTCTAATTCACCCTTAGCTTTATCTGCTTTTAAGTTGTCAATTATATATGTATATTTCTTTGTTGTACTTTCAAATTTAATATCTTCTTTTTTAATTTGATCAGCTTCTTTTGTAGTTGTGCTAATCTTATATACTTTGTTTGTAGAGTCATAATCAGCATTCTTAATATCTGCAATAGACAAAAGTTTAAAGTCTTCATCTGCTTTAATTTCTTCTTTTGTTGTGTTTTGACATTGAGATGAAATTAAAGGTAAAACACCTGCTGTTACGCTTGAAAAAATAAGACTTAATTTAATTTTCTTCTTCATATATTTCTCCTTCATAAGAATAAATTCTTATGCTAATAAATTATCTTAAATTCCTATTATTTTTTAAATAAATGGAAAAAATATCCACATTTTTCAAAATTAATGAAATTAATAAATTTCTCATTTTTTAAAGGAATTTACCAAAAACACTATAAAACAGCATTATTGGATAAAAGCGGTATAAATTGTGAAAATTTTATGTGGAATAACTATGTTAAAATTACTTTTTTTGTTACCAAAAATAATATAATTCTATAGCAATAAATTTTATAAGAGAGGTGCAAATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAAGCAATTCAAGAAAGAGTTGTTAAATTAACTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGAGTTATTAGATACAAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGTAGGTGGAGGTACATCAACAAGCGAAACAAGAAAAATAGAAGTCAAAGCTAAAGCAAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAAGCAATTCAAGAAAGAGTTGTTAAATTAACTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGAGTTATTAGATACAAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGGAGGCGGAGGAACAACAACAGGTGACTATGTTTATGATAAAAGTAATAATTATTATTCTTCTTTAGAAGGTTTAAGAGGAGTTGATTTATTTAATGCTTTATTAAATTTACAAAAACAAAAAAGAAGCAAACTTACAAATTATGCAGATTTAGTTAATAGAATTTATAGAAATGCATTTATAGATAAATATTTCGAAAAAGACAACACTATTCTTGATGTATATTCAGAAAATCCAAATGGAAAAGACCCTTATGTTTTTAAAGTTGATGAATATGATGGAAGAGGCGGAAAATCAACAGGAAAATCATATGGTAATGGAGAAGGATCTATGTTTAATCGAGAACATATGATTCCTCAGTCATGATTTAATAAAGATGAGGCAACAAGATGTGATGCTCATTTTGTTTGACCTACAGATAAAAATGTAAATGCTTGAAGAGGTAATGATCCTCATGATAATATTAGTAGTCCATCTTGAACTTCTTTAAATGGAACTAAAGGGAAAGATGGTGTTGGTTGTGAACCTATTGATTACTTTAAAGGAGATATAGCAAGAGCATATTTCTATTTCAATGTTACACACCATAATGGAACACATAATAAAGGTGAAAGAGTATATACTTCAACTTTTCCTTTCTTTGTAAAACACTATTTAGATGTATATTCTAAATGAGCAACAAATGATCAAGTTGACTTAATCGAAATTGACAGAAACAATAAATTAGATGAAATCTACGGTGGTTTAAGAAATCCATTTATTGATTATCCTGATTTAATTGAATTAATTTGAGGCAATGATACAACTAAAAAATTTGAAAATCGTGGTGTAATCGTTAGTGTAAAAACACCTTAAAATAATATAAATTCAACAAGCAAGAAATAATTTGCTTGTTTTATTTTTATCTAAAAAGCCTATAAAATAGTTGATTAGAGTAATAACACAAAAAATTTATTTTAATTAATTAAAATACTAAATTAAATCTTGCTATTTGAGTTTATAATTAAAATATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAAAAATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGAGCGGTAAATTTTATAAAAGACTTAAAATTAGAATTAAATAAAGTTAATGATATTGAAGGCTTTTTAGATTTAGTTTTAACTCAAAAAAATCAAAAACTAATTAAACAAAAATTAAGTCAAATATTCAAATTTGTTAACTTAGATGGCAATGTAAAAGACATTATTATAATTTACGATTTAATCTGTTTTATAGGTTTATTAGTAAATTTAACAAACACAGAATTTGAACTTTTACCATATGATGCATGTAATATTATTTCGTAAATAAGGAGGTTTTTTATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGAGCAACGCTTTTAGAAAAACTTAAAAAAGAAGGATTCTTAAGAGTTAAAGTTGACGGTGAAATAAAAAACCTTGAAGATGAAATTAAATTAGAAAAAAATATTAGACATACTATTGATATAGTAATTGACCGTGTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGAAGAAGTATATTTGAAGGTGGAATTAAATATTTTGAAAACACAGTCAATACTCAAAATTTAGAGTGACAAGAATTTGAAGTTTTATTAGATCATTACAAAATTGATAAAAATTGTCCAATTGAAGATTTAGGTAAAAAAGACTTAGAAATCATTAAATATGGATCAAAAGAAGAACTCGACTTTGTTGTTGTTTCTGAAAGTGGAAATAAATGAAGAAAACACAAAGTTATCGAAGGTATTTTGACTAAAGTCCAAAGACTTTATATAGAAACAACTAGCGAAAGAATTCGTGACTGACTTAAAAAATACATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATCAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAAATCACAATTTTGAAGAAGCACAAGCTGCTTTTTTTATTTTTCTAATTTGCTAAATTTTGACCAATTAACCTATAAAAAAGCCTTAAAATTCAAAAAATAAATTTTAATTTTTCTTAATTTAAGAAGGCTTGAAAAATTTTGAAAAAATAATACATTTTTTTTCTTGGTATTTTAGATTTTATTGGTAAAATATAAACACATTTGCTTTTTTATTTAATATTAAATATTTATTATGCAAAAAACCTTTTTATGGTATAAGCTGACAAAAAAGCAAATTTTATTATGAGAAAAAAGGAGTATTACTGTATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGAAACGTTCCTGACTTTTTAGTTACAAGTAAAGAATCATTCGAATGATTCAAAAAAACTGGTATTGAAGAATCTTTAAGAGAACACTACCCAATTACAGCGACAAACAAAAAAGTTTCATTAGAATATATTCCAAATTCTGCAGATTTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTGGAAGTTTTATCATCAATGGTAGCGAAAAAGTTATCGTTAGTCAATTAATTAGATCACCTGGAGCTTACTTTGGACGTGGCGTTAGAAATAAACAATCTGATGACCTTTTCAACAAGGTAGAAATTTTACCTCGTATTGGTTCTTGGGTTGAAATCTCTCACAAGGTTACAACTAAAACACCAGATAGCGTAAAAATTAAAATCGATAAAAACAAAAATGTTAACTTAGTAACTTTCTTAGGTTCACTTGGTTTAGATCGCCAAATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGAATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGACCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGATTAATTGATCCTATTACAGGTAACAAATTAGATAAACCAGTTTCAGTTGGTGTTATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGAGCTATTGAATCTTATGGTGCAACAAATGTTCTTCAAGAAATCTTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGATCAAATGGTGAAGTAACTAAACCAGAAACAATTAACTATAAAAGTTACAAACCAGAACGAGATGGTCTTTTTGATGAATTAATTTTTGGACCAACAACTGACCACAAATGTCCTGTTTGTGGTACTAAATATAAAAGAAGTGATGAAGGAACTCAATGTTCAAAAACTCCTTTATGTGAAAAATCAAAGGCTACAATTTTAGCTAAAATTTCACGTCGTAGCCGTATGGGTCACATTCATTTACAAAATCCTGTTGTTCACTTTTGATTCTTCAAAATTGATCACTCAATTATTTCTAAATTACTTGGTTTAAGAGTTGCAAAAAGTTCTAAAACAGTTTCAAAAACAGATTTAGAAAAACTTATTTACTACAAAAGCCACATTGTTTTAGAAAGTGGTGGCATTAAAGCTCTTCAAAAGAATGCAATTATTGATATTGCTGAAGCTGCAGTTATTTATCGTGATGCTTTACTTGAAATTAGAAGCAAATATTCTGAAGAATCAGAAGAATATGAAATTATTAATGAATCACTAGAAGAATTAGAATCATATGCAACTAGTAAAATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGCCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGAGAAGAAACCGATGCTCCTATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGATGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGAATTATTAAAGAATTAATTAAAGGTGACAACCAAATTAACTCAATTAAAGCTGGTAAAAAAGCTGTTGAAAATTTAGATCCTATTATTTGACCTTATGTTGAAAAAGCAATTCAAGGTCGTCCAGTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGCAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAACGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGTGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGACATACAGAAAACTTAGTACGTATAATAAAAGAAGAATACGAAAAAATTAATGAAGAATTAATGATTGAATATCGTAAAGTTGATCAAGAAATTGGCTGAAGAGTTAGTGATTATACAAAAATGCTTGAAAGAGTATGATTTAGATATACAAACTTTGTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGAGCCGAAATTAAAGACTCAATTGAAAAAGACTTAAAAGAAGTTACAAAAACCGATCCAGATAACCCATTATTTATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCATGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTGTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGAGGTAAACCAGCTGTTATTGCAGAACACTATGGCAAGATTACAAAAATAGAAGATAAAAAAGATACTTTAGGTAATGCTAGTGATTATCAAGCTATTACTCTTGAATATGAAGATGAAGAAGGCAATACACAAAGCAAAATTTATGCTGCTCGTAAAGATCGTCGTCTTAGAGTTAAAAAAGGTGATAAAGTTATCCCTGGACAAAAAATTGTTGAAGGACCTATTATCCTTAATGAATTACTTAAAGTTGCTGACACTCGTTCAGTTCAAAACTACTTATTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAATAAATAAAAAATAAATAGCTTTTACCAAAAATATTGTTTTATAGTACTATTAGTAAAATTTAGCTATTTATTTTTATTTTTTTGTAAAAAATACTCTTTTTGACTAATCAAGTTATTTTATAGTATTTAAAAAAACAAATTAAATTTGGTATATAATTAAAGTCCGGTAATATTTAAAATATTGCCGGGTGAATTTAGGCGCCCTTCACTCAGGCGCAAAAAATTATTGTTATCATTTATTTCATTGAAAAATATTTTTATCGATTTTTATAACTTTTATTAAATTACAATTAATTACATTTTCACATATCATTTATTTATATTTGGTAGTTTATTAAATCATAATGTTTAAAAAACTCTTTTTAAAGTGTTGTTAGGGAATTTTAAATTCATATCAATATGCTCCTTTCTGTGGATTTCTTTTTGTTTTAAAATAGAAATTTTGCATAATAAGAAAATCTTTAAAATTTCTTATATATATTGTTTTTTGTTAATATTATTATCGGGTATACATTTTTTATCCTCCTTTTAATATTATATTTATATTATGAATAATACGCGAAACAGGCGTGGCCTGTTTCGCTTTTATTATTTTTTTAGTATAATAAGGTATATTTATTACTTATAAATAAATTTAAGTATCTTAAATTTAATTAGGAGTCATTTATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGAAATTTACTTTTAAATTATGATCCAAATAAACCTTTAGACCCAAAAGATTTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGCGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGAAAACAAAATTTGAAGTTATTAACTCCAAATATTTTTGATATACACCCTTCAAGTTTTGGCTACAAAAAAATGGCCATGGAAGTATTTGTTAAATTAATTAATCCATCTCGTGATATTAATAAATACCATGAAAATAAAATTGAATGAGATAATAAATACTTACAAAGCGACCAAGATTCTTTTGAATACCAAATCGAAGTTAAAAATCCATTTGAAAAAATCAAAACTATTTTCAAAAATAATTATGATGGTTACTTAAAAGAAAAAGATAGTTTCTTCTTAGCTTATGAAAATCAATTAAATGAAGGCAATTATATTAATAGAGTTTTAAACAATAATGGTTTAGCTGATTTCTTATTTGTTGATTTAATTAACTCAATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGATTCTTTAATGGTCAATTTATGGCTAAATTTTTATATGATGTTCAAAAACAATTATTTGAACAAGACTGAGATCAAGATAATTTACCAGGAGCTAAAGACTATCAATTTAAATACCTTTTAGAAGCAATTAAAACACAAACTTTAAAAGAAGAAAGAATTATTGAATTTATTACAAGTTTTTGTTCAAGTTCAGTAATTACAACTCAAAAAGATGAATTAAAAACCATTTTAGAAGAAATCTTTTCAAATATTGTTGCATATGAAGTTAAATATGAAGACATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTCGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGACAAGAATCAACATTTAATATTATTAAAATTGTTGCTAAATCTAACCTATACAAAAATCGATCAACAATTTCACAATTAATCAAAAATATCTTCAATTACTTAAATACTAAAAACTCATTTGCTAAGAGAATTGTTGATCTATTTTATGACAAAAATCAAGCAAAAATATCAGAATATATCTCAAAAGAAAATCTTACTAGTTTATTCGAAAAGGTCTTTAAAACAGAAGAATTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGACTTGCTAATAAAGGAAGTGTTGACTATATTGTTCTTAAGGCTTCAAAATTTATTCTTGATGTTTCACAACAAAGTTCAATCAAAGGAATACTAACTTCATTAATTTATGAAGCATTATCTAAAGAAAGAAATTTAGTTAAAGATATTGATCAACAAGAAATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGAGAAAAGAATACAGTTAAATTAATTAAAATTGCAATTACTAAAAAAACAATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGACTTGAAGCTATAAGCAAATTCATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGAAGAAAAGAACACTGAACTAATAGTGAAAATGATGACATTATTATTCAAAAATTCATTCGTTCATTTGGTATTGGATTACTTAAAACAAATATTTTAGATGATATTGTAGATAGCATTTTTGAAAGATTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGAAGATTTTATAGCTTTTTAGCTTCAGTATTGTATTCAAACACACCAAGTGGATTGTTTTGAAATGGAACAAGTTTGACAGCTGAAGCTTACTTAATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGAACTGGAACTCTTCATAGTCGCTCAAACAGTTTAAATGACTCTAGATATGGTAGTGATCATGTTCTAACTTACATTTATTACAAAGACAAAAAAGATTCTAAATTTACAAACAAAAAATTCAAGCAAGCATTAATTGAATATATGCATCAAGGTTACATGCCTTATAAATAAAAATAGAAAGAAGATATTATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGAGCAAATGTCGCAATAGGCACTAAAGCAACTTTAATTACTAGTGACAATAAAAGATATAGCGGACTTTTCGGACACACTTCAGTTCACATTATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAATATTTGTGTTAAATAACTTTTACTTTATAATATTAAATAAGTTATCACAAAAACGAGGAGGTTAATATGGGTACAGGTTCATGAATCGGATTAGTTATTGGTATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAATAATTGTAATAATTTGCAAGCAAAATAAAATATTTGCCCTAGTTAGTGAGCAAATATTTTATTTTTTCTTTTTAAAGAATCATCTTCTTTTTGGTCTTTTGTTTTCTTCTCCTTCAACACTACGAGCATAGTGACATTTAGGGAAGTTAGCACAACCAACAAATTTTGTATCATTTCTTTTATTATTACGAACAATTAATTCACCGCCATCATCTGGACATTTTTCATTTAAATATTCAAGTGCAATAGCAGTTAATTCTAATGTTTCAGTTGCATTTTCATAAGTCTTATTAAAACGTTTTCAAAAATCATACATAATAGGTTGAACAGTAGTTTTATTTGAAGCAATTAAGTCTAATTGTTCTTCAACTTCAGCTGTATAGCCTTCATTAATAATATCTGGAAAACCATCTAATAATTTACTTAAAACTAAACGGCCAAATTCTGTAGGATGTAAACTTTTATCAATATTTTCAAAATATTTTCTTTCCAAGTTTACTGCAACAGTAGTTGCAAAAGTTGAAGGACGTCCAACTTTAATATTATCTAAAGCTTCAATTAATGAACCTTCAGTGTAACGAGCTGGTGGTTTAGTTTCATGATTTGTTATTTGATATTCTAAAACATCCACTTTTTGTTGTATTTGATAATTAGGATCTTCAATATCTTCATTTTCATTTTTAACAAGATAGTAACCTGGGAATATGGTTTTACTTACATTAGCTTTAAAAGTTAACTTATTTTTTTTAAAGGTATAAGTTTTATTAGCTTTAATTGGTGGATTAATTAAAGACATAAGTGTGTGTTCATAAATTAGCTTATAAACAATGTATTCACTATTATCTAAATTAAATTTAGCTTTAGCAACTTCAGGCAGTAAGTTTATATCAGTTGGTCTAATAGCTTCATGGGCATCTTGATCGCCTGCAAAGCCTTTGATTTCTTGAAGAATATATTCTTCACCGAATTTATTTTTAATATAACTTCTAGCAATATTTAAGAAATGTTGACTCAATCTAGTACTATCAGTTCTAGGATAACTAATCAAACCACCATCACCATAACCTTCATAAAGTTTTTGAGCTGCAATTTGAGTTTGTTTAGAAGTTAGCGGACTTTTCTTATATAAAACTGCTTGTTTAAGTGGCGTATATTTAGCTAAAGTTTTTCTTGCTTCTTTAATATCTTCTACTTCTAAAACATTAATTGGTTCAATGCTAATTTGTTCTTTAACTTTTTCTAATTCTTCAGGATAAACTCAGTTTTTTTCTGCTGCTGGATGTTTATCTATATAAATAGAAGCACTTAATGTTTCATTTAATTTAGCTTCAACTTTGTGATAACCACGAGGCACAAATGCATTAATTTCATCTTCACGATCAACTACTAATTTAAGAGCAATTGATTGTACACGTCCTGCACTTGGAGTACGAGGACTATTTGAAATTTTCTTTTGCATTAATTGGCTTAATTTGAAACCAATAATTCTATCAAGCATTCTTCTTGCTTTTTGTGAATTAATTAAATTTTGATCTAGTTCTGTTGGATTTTGAATTGCTTTTTGAATAGCTTCTTTTGTTATTTCATTGTATTTAATACGGTAGTATTTTTTCTTATTTTTATTACGATCTTTTTCAATTAAAAAGTTAACTATGTGATCTCCAATTGCTTCTCCTTCTCTATCAGGGTCAGTTGCAATATAAATAACATCGGCTTTTGCTATGGCTTCTTTAAGGTTTTTAATAACATTTTTTTTACTATTGTCTAATGAGTAAAAAGGCTCTCAATTTTCTAAATCAATTCCTAAACCTTGTTCGCCTTTCCTAGTACTCATAATAGCGATATGACCAACAGAAGCAATCACATCATAATCGCTTCCTAAATATTTTTTAATTGTCTCAACTTTGTTTGGAGATTCCACTATAACTAAATTATTCATAACTTTAATTTATCACAATCTTGCAATCTTGTTATCATTTTTTTAGCTATTTTTTTGCCTTATTATATTTAATATTAAAGTATTAATATTAGAGTCAAAAATATTTAAAAATTTATTTACACAAAAATTTCACTATTGAGCATTTAACTTAATTTGCCTATTTTATAGTGCTTTTAGTCAAAACTTCAAATTTACGAATTTATATTTTTAAATATAATTTATTTAACATAATTCTTTTTATTGAAAAATAGTTATTTTTTTACTAGCTAGTAATTAAATTATATATATAATATAACAGAGTATTTTATTAGGATAATAATACTCCTTGGTCACTTAAGACCCTGAAGTCCAAAAGGAGAAAGAATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGAAGACATTTAGCAATTAACTTAGATTCTGAAAGAGGATATGGGAAAGTTAAAAAAGAAAGCCATAGAGCAAAATATGAAGAAAAGAAAGAATTCAAAAAATCAAATAAATCAGCTGAAGCTGGTGAAAAAGTAGCTGAAGAAAAAGCTGCTAAAAAACCTAGATCAGCTAAAAAAGAATCACAAGAAAAAGAAGTTAAACCACGTCAAAGTAAAGCTAAAAAAGTTGAATAGTTAATTAACTTCTCGTAAGGAGAAAGCATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGAAGAAATTCAGCAGAATTTTTAAATAACTATGCTTCAAAAGGAGCTCTTGTTTCAATTGAAGGTTCATTTACTTCATCACAATATGAAGGACAAAATGGAACAGTTTGATCATATGAAGTTACAGTTGACAATATTTCATTACTTGAACCTCGTTCAGTAAGAGAACAAAGAACAGGCAAAAGTGGAGAATATTCAGCAACAACTCCTTCATTTAATCAAGCAAATTATCAACAATCTAAAAATCAAAATACAACTAAAAAAGCAGCATCAAGCCAAACTAAAAATAATTCAAAACAAAATGAAAACCAATTTGGTGGATTTTTACTTGATGGCGAAGATGAAAATGAAGATAGCTATCATACATTTTCAATTGATGTTGATGAAAATTAGTAAGGAGAATCATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAATTTAATTATTTATAAATATTAATTTAATCACCTAACTAGGTGATTTTATTTTACTTATTCACTTAAAAAATAAGTTATATAATTGAAACACTATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGAACTAAAGAAGCAAATGATTTAGCCTTAAAATATTGACCTGGAGCTTATTCTATTATTACTAATAACCAAGGTTTCAGAATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGATTGCGATAAAAAAACTGCCTAAGGCAGTTTAAATTAAATAGTTAATTCTAAATCTTTTGAAACAGCTTCATAACTTTCTTTATCATTAAGATAAAGCAAGTTAAGAGCTGCTTTTTGTTTAGCTAAAATAACATCAGATTGAGCTTTTGTTAATTCATTTGAGCCAGTTGAAATTAAAGTTTTTTCTTTAATTGATTTGTAAAATTCTTCTGCTTCTTTTTCTGTAATTTTGTTTTTAATTAACAATGAAAAAGTAAATTCAATAATTGCGTCTAAAAGAGAATTACGAACATTTGTTTTTGCTAAATCAAAAATTTCATAACCATCTTTTTGTTTATTTTCTATTGCATTTCTTAATAGATTAGCTTTATCTGGAGGTAACATTGCTAATTTACTTTTAATAAAATCATTTACTTGTGTATCTAATAAAATAGGTGGTAAATCAAAACCATATTTATTTCCAACTGTTTCAATTGCTTTTTTCATGTAATCTAATAAATAACTTGAAGCCATTTCATTTTTCAAATTCATTGACAATTGATTATAAAGATCATCAAGAGTATTAATGCCTTCAATTTTGATTTCTTTTATGTTGTCATTTGTTAATTTAGTTTTAATTGGACGAAAAACTTTAGTAACTGTAATTTTAATGTTTTCATTTTCTGGTGTATTTACTGAAAATGATTCATTAACTTTACGATTCATTAATTGACTATTAATTGAGTTAGGATTTTCATCTTTTACAATTTTTTTATCTAAATCTTTACGTGACAATATTTCTTTTTTGTCTTTAGAAACTGAAATATTATATCTAACATAATCATTTTCTTTTGATTGGTCTTCAACTTCTTTAAGTAAAGGATAATCATCAACAAATTTTTTGTAAATTTCATCAATAGCACCTTTTGTCACCATTTCATATTTACCATCTGGTTTAATATTTATGTCAATTTTATCGAATTCAGCTTTTGGATAAATTACAATTGTAACAACAGCTTTAGCTTTATCTTTAGTAATATCTTCGACAACAACAATAGGCGAAAAAGCTAAACAATTTGGATATTTTTCTTTTAATTCTTTACCCATTGATTCAACTATTTTATTTGTATCTGATTTAAAAGCTTCTTCAATTAATTGGTCAGGTGAAATTTTCTTGCCTTCTTTAATGCTTTCCATTAAAAAATTATTTTGAATTTGTTTTCATTCTTGGTCTTTAAATTCAATGGTTTTGGTTTCTAAAACATATTTAATCATTTTTCCTCTTTTCTTTATTTATTATATTTAATAGTTCATGATTTTTTCATATTCATCTAGTGTTACATGATAAATAAAACTTTTATCAGGAGCAATTTCAACAATAACATTAGCTACTTGGTTAACTAAAGCTCTATTATATGTTGTTACAATAACACCACCCTTATAAGCTTTTAATCCTGCAATGACACTGTCAATGCTTTCAGCATCAAGGTGATCTAAAGGTTGATCTAGAATCAAGAAGTTAGATTCTAAAAGCATCATTCTTGAAAACATCAATCTCGCTTTTTCTCCACCTGAAGTTACTTTAACATTTTTAAATACTGAATCATTTGAAAATAACATTCTTCCTAAGAAGCCTCTCATTCTTTGATCACTAACATCTTTGGTTTCTTCAGTTGAGTTGTATGGAGGTCATTTAGCAATTCATTCTAGAATTGTTATATCTTCTTTAAAATATTCTTTATTTTCATTTGGATAATAAGTGTGTTTTATTGTTTGGCCCCATTTTATAGTGCCTTTTGTTGGTTTTATCTTACCAATTAAGCATTCTAAAAATCTAGTTTTAGCAATATCATCTTCACCAATTAAAACCATTTTTTCACCACGAGCTAAAGTGAATGAAACATTTTGGAATAGTGTATTTCCTTTTTCATCAACATAAGTCAAACCTTCAACAGTTAGAATGTCTTTACCAGGTTCACGGTTAATGTCTCAATTAATATAAGGATATTTACGATTAGAAGGTTTAATTTCATCAAGAGTAATTTTTTCTAAAGATTTTTTTCTACTGGTTGCTTGTTTACTTTTAGAAGCGTTGGCGCTAAAACGAGCAATAAATGCTTTAAGTTTTTCAATTTGTACTTCTTTTTTAGCATTTGATTGTTTCATTAATTCTCTAGCTAACTCACTACTTTCTTTTCAAAAAGTGTAATTACCTGTATAAATTTTTGCTTCATTGTAATCAATGTCAACTATGTGAGTGCATATGTTATCTAAAAAGTCACTGTCGTGGCTAACTACTATAACAACATTTTGATAATCTGCTAAAAAGTTCTCTAATCATTTAATTGCACGCAAGTCAAGATGGTTAGTAGGTTCGTCCATGATTAAAATATCAGGATTACCAAATAATGCTTTGGCTAATAAAACTTTAATTTTTTGATTAGCAGTTAATTCTGACATTTTTGATTCTCATTTATTTTTTGGAATTTGTAAACCTGACAATAATTCTTGAGCATCATTTTCAGCAGTTCAACCACCTAATTCGCCGAATTTTTCTTCTAATTCTCCTGCCCTTGTATAGTCTTCCATTGTTGCTTCAGGATTTGCATAAATAGCATCTTTTTCTTGTTTGATTTTATATAAATCAGTATTTCCCATAACTACAACATCGGTTACATTCATATTGTCATAAGCATTATGATCTTGGCTTAAAACACTAAGTCTTCGGCTTTTTTCAATTATTATTTGGCCGCCGGTAGCTTCTATTTCACCAGCTAAAATTTTTAAAAATGTACTTTTGCCAGCTCCATTGGCTCCAATTATTCCATATGTATTTCCAGCTGTAAATTTTAAATTAACATTTTCAAATAATTTTTTGTCGCTAAAAATTTTACTTAGATTATTTACTTCTATCATTTTTCCTCCTAAAGCATAATAATTATTTCAATTATAAATTAAAATAAATTTTAAATTTAAAGTTAATAAAAAAAGCTACAAACTATACTGCAATAGCTGCATCTCTAGCAATTACTAAAAATAATTGAGGTGCATTTAGTTGTAAAAGTTTTAAAGCTATTTTTATATTAATTAAATCTTCTTTTACAGTGTTAACATTTTTGTTTTCATTTCTATTGTAATAGTTGTTTGATTGAAATAAAATATTTTTGCTTAGATTTAATTCTTGATTTACTTCTGTGTCATGGACTTTTATTTTTTCTAATGTGTAAACTAATTTTTTAACAATGTCTTCAACAATTTCTCTTCTAGCACTATTAATCGCATTATCTATTATTTGTTTACCATATTGATCTTTTTCTTGAATACTTTTTTCAATAGCTGCTCGATTATTTGGATTTACTTTTTCTAAATATTGATGAGTATAATCTGATATTTTGCTATTTATAAATTCATCAGGAATAATAATTGTATTAGTATTTTCTAATTCACTAATTAAGCTGTCAAAATATCTCATAACTTCTTTAATGGCATTTTGTTTTTTAGTCATTGAAAAGAAAAAGTTTTTTAAAGTAATTAGATTTGTAACATAAGGTATTTTAAGAAAATAAATATTTTGATTGGTTAGTGGCATCGAAACAGTCCTTTTGATTTCAATTACTTGCATTTCTCATTTTCTACCTAAAGGATCATTGATGCTAAAAATTTCACCATATCTATGATTAAGAATTGAATTATTAATAGTAAAGTTGTTTGTGTGGTTAGCTATTATAAGAACATTTTTATCATCTTTCATTAAGATATTATTTTGAAATAATTTAATGTTTAACTTAACTAAGTCGTTTTGTTTAATAAAATCATTTACTGAATTTTTAAATGTGTAATTTTTAATTGTATTTTCAAAAATTGCACCAATTGAATTTTCATCAAAAAAGTTGTAAATATAATTTACTGGAGTATTTTTATAGTTAATTTTTTTAAGCTCTTCAAAAGGATAATAAGCAACCACAATTTCCGCACTAATAGTGTTATTTGAAATAATATCTTTTCTAATTGCTGGACTAGCAATATAGGCTAAAGCATCACTTTTTAAAATTTCATTTGAAATTAAGTTATATATTTTTTTGCATTTATAATCAAAAGATTTTTCTAATAAAGACATTGAATCAATTTTAGTTCTGTCAATTTTTAAATCTCTAACAGTATTATTTATCATTGCCTCAATTTCATCTTTATTCATTTTAATATTTTCTCTTTTTAATAAATAATTGATCATTCTTCCTCCAAATCATTTAAAAAACCTAATATTTCATTCTTATAATATTTTAAATTATTTATTAAAATTTAATATTAAATAATATATAAACCACATAAAAAATCTCTTTTTAAAATAATTTTTTTACCAAAAAACCTGTTTTATAGTGTTTTTTGTATTTTTGAATTAAAAAAATAAAAATTAAAATATTTCTTTTTTTCTTAATTTATAAGCTTAAAAATTGCTAAAAAAATATTTAAAATTTTTATTTACAAAAAAACTTTTTAATATATAATAATAAGGCATAACTTAACAAATGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCATTTGGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCATTTTGGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCATGCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCATTTATATGCTTTGCTATAGTTAGAAAAGTTTTCTTTTTTTATTTAGATGACTTTTGCAGGTGCAATTCCTTCCAAAAAGCTAGAAAAAGTTGCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCAAATGCTTTAGCACTTAACTTTTTAATAACATCAACTTTGAATGGTTGGTGTTTTTTTATTAATTCAGCCATATCTTTTAAATAATTTCTTATATTCTCTCTTTCAAAAACTTTACGACTTCCAATTAAATAGAAATTGATATTATTGTCTGGACTAATATTTCATTTAATATAAGAATTGTTGCCACAAAAAATATCAATTGTTATATTTTTTTCAAAATAATCAACTTGCAAAATTTTCAAGTTGTGTCTAATTGCTGCATTTTCAAACATTTTAAAATTAATTATCTTATTTTTCATTTTGCTCCTTTTTATTAACTTATCTTTTTTGTTTATAAAAATGCTAAAAACTGGAAAAATTCAGGAAAAAGAGATCAAAAATACCAAATTTTTCCAGAGATAAAGTTTTTTATTTTCTATTAAAAAGGTTATTTTGAAAGGAGATTAAATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGATCTTTATATGGATTTAAAGATTTCAAAGAACGCTTATTATTTATGGACTTAATTAATTTAAATGGAATTGGACCAAGAGTTGCTTTTAACATCCTTAACCAAGGATGAGAAAAAGTTGCATCTTTAATTGCTTTAGGTAAAGTAGATCACCTAATTGAAATTCCTTATATTAATCCAAGAATTGCTCGAATTGTAGTTGGCGAATTACAAGACAAATGAGCTAAAATAACTAATACTCAAGATGCACAAAAACAAGTTAAAAATTCAAATTTATTAAGTGAAGCTAAAGAAACATTAAAAATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGAGGTGCTGTAAACTAATTGTGGGAATGAATTTAAATGAGAGAACAAGCATAAAGCTTGTTTTTTTCTTCTTTCTATCAAACTAAATATAGAAAGGAGGTGAAATTATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTTTTTCAAAACTATGGATTAAATTATGACAAATTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTATAAAATTCCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGAAACAGTAAAAAGTATTTAAATTTTGCTGTTAAGGCATTGATTTTTTTATCTACTCTATTTAAAATGAAAATAACAATATCAAAAAGAATTTTTGATCAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGCAAATTGATTAAAACCATCAACAAAAACGATTCTTGATATACAAATTTTACTTTTGTAAAATTAACTTATAAAAATTGAAAAACTAAAAAAGAACAATATTATTACTTAAAAAATGAAGAAGTTTGAAGATTATCCCTAATCTCATCATTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTAATAATAAAGTTTTAATTAACAATAAGTTTTTAAATTTATCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAACTTATTACAAAAATGACTTTGGAATGTAATGCAGTTGTTTATCTAAATGACTGAAAAATATAAAAAGTAGGTTATACAAAAATTCACTGATATTTTAAATATCTGTTTTTTAATGTTGCATTTTGAGAAAAACACTTAACATTATTTAACTTTTGATATTTGTCTATTCAAAATTAAATGAATAAGTTATTAAGACTTTGGAATGTAATGGAAAAGTCTTAATATATGAATCATTGATTCATAAAGTAATTTAAACAACTATGAAAAACCAAAAATTATCACAAATTACGAGAATTTATTTAAAAATTTGCAAAAAATCTAACAAATCTTGATCAAAAATTAAGAATTTTAAGAATATTTATGATTTTGTGTTTGCTAAATCATCAGAAAAATATTTTTTTATCGATTTAAAACATATAAATGATTCAGAATTCAAGCAAGATTTATGGAATTTTTCGAATTCTTACAGAGTTTATGCTTGTTATCAGATTTATGAAGAAATAAAAAACAACAGGAGATCTCTTGAACAAGTATAAATCAATCTCTCTCTGCTCTCTCATTAGCCAAGCAGCTCGGCGAGGCAATCAAAAATGGACCGAGCAAGCAAGCATGGAGCCAAATCCCAAACTAAAGCGGAATTTTTAAGTTAAATTGTCAGAGAACCACCTTCAAAAACTCTCTAAAAAATAACCTAAAAAACCACTTGATAATCAAAATGGTGCTTGTTGTTTAGTTTTAGTAGCAATAACCGGTACTATGATGGGAACCTATACAGCAACGCCAATAGTTTATGCTAGTTATCAGATTTATGAAGAATTAAAAACAATAGGAGAAAATTATGGAATATAGTCAATTTGAAACACAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATTAATAAGCCTTCTAATTTTGTTTCACTAAGCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGCGCTAAATGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACTAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGATTTTTAGATAATGATTATATAAAGAATATTAGATTATTTAATAATTTTTATTACGAACAAGTAGACTTAAGACCTTATTATGGAGCTGATTTCTTTGAAATATTAGATAAAAGAGAAGATTGATTAAAGATAGAAAGTTTTATAAAAAAATTTAAAGAACACAATCAAAATTATTTTTCAAAAATGCCAGATTTAGATCAATCAGAAGAAGCACTTAGAGCACTGCTTCATTTGGAAAAGTCAGAATGAACCAAATTAAATTCAAATAAAGAAATTTATAGAAATATTAAAGAAAATATTTTCAATTTAAATGATAAAAATTCTAATTTATTTAAAGCAATTGCAAAAAGCAACAAATCTTACTAATTATGAAAGAATTAATAATTTATTTAAGTTGAAAATATGAAGGAAATATTTGAAAAATTTATGAATTTTTAAAAACTTACAAAACAGTTGCTCAAGCTAAAATAGATGAAGTATTAAATGAACTAAAACAAAAAGGCATTAAGTATATAACAACTTTTGATGATAATTTTCCAGAAATATTAAAAAATTATCGCTATTCACCATATGTTCTTTTTTATAAAGGCAATATTAAGATTTTGAAGTTACTGGAAACTCAAAACAAAAATGAAGTTTTCATAGAATGAGATTTTGAAAATAAAAAAATTTTATTCACATCATTAAATAGTAAAGGTAAATCTAAAATCATTTACATACTAGATTGTGGATTTAATCATCTTAACAAAAAAATTAATTATAAAAAAGAGTTGTATATCACACAATTTCCATTTCAAACATTGCCTAGCAAAATTAATCAAGAATCAAGTGAAAAATTATTAAATTGTTTATTCAATGCATAAGTACAAAGGAGAACAGATGTCAAATTTAAGAGAAAAATTAAAAAAATGTAGTAATGAATCTTTGAAATGTTTGTGAGATTTAGTTGGCGATATATCAGACTTACCATTAAGTGATGATATTAAAAAAGTAGTTGAATGTCTTGAACAACCTGATTATTGTGAACATTATAAAACTCATGAACAATTTTTAAGAGAATCATATAAAGAAATAAAAAACAAAATAAAAGAATATAACACCTTAATTGAAACTGCAATAGAATCATTAGCATCAAACTTTTCATTATTTTACTTAACAAAATCTATTAAATTAAAACTAAAAACAGTTGAGATATACGTTAAGAATTATAAATATAACGAATGAGATATAGAAGATGTAAATGTTGAAATTGCAGCAACAAAATTTGATGATTATTTAAATAATTTAAACAATGCAATCTATGATGAATTAGAAACAAGAAAAAAAGAGAATGATTAAATGCATATTTGTAAAAAAATATTGTTATCAGAAGAAGCAATTAAATTAATGTCTAATAAACCAAAAACACTATAAAACAGTCATTTTAGTAAAATGTATAATTTAAAACCAAGAAATTTTGAAGTTTAAAATTAATTAATGAAAGGAGTAAAAATGAAACGTAAAAATATTGTTATATATTTTAAAGACAAGGAAGAGTATAAAACTTTTAAGCAAGTATGGAAACAAATCCCAAACAAAAGCGAATATATTAAACAAATTCTCAAAGAATTCAAAAAATAAATAAAAAATCAAGAAAACTTTGGAAATGCTTGTCAGATAAAGCTAAGGTTGTTCCGATAGATAAATCTAAATTATTGAAAATGAAGCAGTAAAGAAAGTTAATTTAAATCAAGACATAAAATTTTCACTAGAAAAAGCAATTATGTTTAATAAACCAAAACTACTATAAAACAGTCATTTTAGTAAAATATATAATTTAACACCAATAAATTCTGAAGTTCAAAAATTAATTAATAAATCAAGTGAAAATATTAAATTGTTTATTTACTGCATAAGTACAAAGGAGAAAATATGACTAGAAAAGAATATAAAGAAATTATTAATATGAAATCTAGAACTTTAAAAATGGAATTAGTAATAGATTGATTATTAAAAGTTGGTATCTTCAATAGAAGAGAAGCTTATTTACTAGATACGAAAAATTTTGAAGTTCAAAAATTAATTAATGAATCTATTAAAAGAAAAACAAATTATAAAAACTGACAAGAATTATTTAAAAAAGAGCTTGTTAGATAAATATGGAATCAATTATCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAATATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAAAAATTTACTTGAACTAAATATATATTATGAATTACTTAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAGAAAGGAAAAAAATATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAAATAATGTTGGATACTATGTTATGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTATTTATATGAAATTAAAAATGATTTTACTTGATCTTTATATGGTTTTAAAGAATTAGAAGAAAGAAAATTATTTGCTGATTTAGTTAATTTAAATGGCATTGGTCCTAGAGTAGCATTTAATGTTTTAAATGTTGGGTTTGATAAAGCTATAAATTTAATTAGAGATGGCAACATTGAAGAGATTGCAAAAATAGAATATATTAATCCAAGACTTGCAAGAATAATTGTTGAAGAATTAAAAGAAAAATTATCATCAAAATCAAAAGATGTTAAAAAGTTAAAACTATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGAAGCAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCAATAGAAGAAGCAATTAAATTAATGCCCGATAAACTAAAAACACTATAAAACAGTCATTTTAGTAAAATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTACATCCTATAACTAGTAATAATATTATTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTCTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCTAAATGAGAAGGCTTTAAAATATTCAATTTAAAGAAGTATTCTTTAAATAATCTTAATCAAATTATTAATTACAATAGGATAAAAACTAATTTTAAATTAAGCAATAGTCTTGTTGAATATATTCTCAATAATTGTAATAATAATCCTGGAATATTAAATGAAGTGTATTTGAAAGCTATTGATTATTTTGCAATAAAGAATAAAGAAATGAATTTAAAACAATTAAAAAAATTAGTAAATAAAACAAACAAAAAATATGAGAAACTCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCGATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTGCCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACCTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAATCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGTCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGTGTTAATTGAAAAAATAATAATGATATTGATAATTTAATTCTTGCATTGTCATTAGGGCTTATGCATGGACCTATGAAAAAGAATGGCACAACAATTTATTTTTCATTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAATTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGAGAAATTTTTCTATCTAAAAATTATGAATCCATTTTTAAATATTGAAATTCATTAAATAGTTTAAATTTTTTAAAAAATAATAGTGTTGATTTAGTTATTACTTCACCACCCTATTTATCTTTAGTCGATTACACAAAAAGTAATTGATTAAGACTTTGATTATTAGGATTTGAAAAAAATAATTTAAAAAAAGAAATCAAACTTTCAGATTCGCTTGACTTAAAAGAATACACAAATTTTATAAAAAAATACTTAATAAATATTTCAAATAAATTAAAACCAAAAGCTAAAGTTTGTTTAGTAATTGGTGATGTTTATGATTTTGAATTAGTAGAAAACATTTGAAAAGAAATTAAAAATGAAGTTCCTTATTCATTCCTAGAAGTAGGAATTGATACCAATACAAATAATTTTAAAACTTCTAAATTGCTTAATTCTCGTAAGGGCAAAGCAACTAAAGTTGAAAAAATTCTAATATTAGAAAAACAAAATTAAAGACTTAAAAACAAGGAGAAAATATGAACACACCAAATAATAAAATTAATATTTTATCAACCATACCATTAAAAATTAAAGGTCGAGCTTTTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTACAGCAGAAGATGATGAATGATTGTTTTTTCTTGCATGTTATCCCAAAAATGCAAAGGTAAAAAATAATAAAAAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGGTGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATTAGCGCCAGATGAAAGTTATCCTTTTTTGAATTTTAAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACCAATGGTGAATTATCTAAAGCACCGAAAATAGTAATAATAAACAAAAAAGGCGACAAGTTTTTAGATATAAAGAATAAGGAGTTAATAAAAAATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTAATTATGAGAAAACCGAATAAATTTTTATCTAAATCTAAACCTTATAACAGTAAAGAAATTATAAATTTAGAATATTACAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAAAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGAATCAGAAAAAGTGATGGTAAATATCCTAAGAAAAGATATGAAATTGCTTATAGCTTATCAAAGCAATTAGAACTTATTAAATACTGTTTTGATGAAAAAATAATATTAAAAGGTGCTGTCGTTTTCATAAATGATTTTGAAAATAAAAATCCAGATAACTTTTTAATTCGAAAATATGATTCAGACAATGGAATGGACAAAAATTGAGTAAGTTGCTATTATGATAAAGATTTTTTTGAAAAATATAATTCTTTAAAATCTTGAAATGATTTAATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGAAAAGAAAATTCTGACAAAAGAAGAAAGGAAAAAAATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTACTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTTTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCTCCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGAATACTAAAATATTGTGGTGAATTAAATTCTAATAAAAAATTTATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAAATATTATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAGAAGAAAATAATACAATGCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGATTAGTTAATTTTTGATTCCTATTTACTTTAATTCTTTTTTGTATCATATTATTACCTATTTCTTTAGTGTTAGCAATTAATTCAGAAGATGTTTTACTATTAAAAATGTCTTCTAAAGCTTTATTGTGAACTTATACTATCATTAATGTTGTAATTACTGTTTTTGTTTTTTATAGATTTGTTTCTTTATGAATTATTGCATCTAAAATGCATAAGAAACATACATTAGATATGCATAACAAAAAACTAGGCAGAAAAGCAATATTATGACCATTTATTAGATATAGCAAATTAATTAGCTAATTTACTAAAAACACTATAAAACATTCATTTTAGTAAAATATATAATTTAACACCAAGAAATTTACAAAACTTCAAATGCAACAAAATTTAATAAAATCATTGATTATATTGCTTCAAAAAAGCAAATAATTCCAAATATAATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCAAATGTAATTTCTAAATTTAAGGATAAAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAATTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATGCAGTAGATAACTTAACACATAACAATAATAAGATTACCAATTTTATACAAATCATTGCCTGAAACAATCAAGCAGAAATTTTAAAATCTTTTGATTCTAAAGGTTCAGTTATTCCAATTGTTGGACACATTACAACTTCTAAATATGATGACTATGGTAAAACATTATACACGCAAGATATAGTTGCTGAAAACATTACATTTCTTGAATCACGTGCTGTTAGAAAACAAAGAACAAATCAGTTGAGCAGTAAGGCAATTACACCGCCAATTAGTTAGAACATTCCTAAAGAACTAATAAATGAAAGAGGTTTTCGGTATTCAGACGATGATAAATATTAATTGTGTATATGCGACTGTTGAGCGTGCTAGAGCGTGTAACATGTGCTTGAATACTAAATGCCCATGTGCTAGCGTGTGCAGGGCGTGCTTCACTGTGCGCATTCATATGCGACTGTTGAGCGTGTAAGAGTGTGTAACACATGCTTGTATACTAAATGTCCATGTGCTAGCGTGTGCAGGGCGTGCTTCAGTGTGTGCATTCAAATAAGGAGAAGATTTATGTCGAAATGATTTTACAGATTACGTGGCTTCAAGAGTTATGACAAAAACGCCAAAACATTTTCTGCTGGCAGCTCTAATGATTATGAGCAAGCAATATTAATTGCTAGAGGCCTCTTAAAGAAAAAAGAATATATTAAAGTCACAATAGAAAGATCACAAATTATAAAAACAATCAAATAAAGAAGAATAAACGTCTGAAAATACACAAAAAGTACATTTTAAGTACACAAGACATTGATATAGAGAGCAAAGCAATTTTTGACCTGTATTATCTATAAATAGATTAACAAAAGCAAAAAATGCAAAATAAGCTTGCATAATGCTTGAGCAAATATTTTATTAATATATAATTTAAATAATAAAAAATAAAATTAGGTGAGGTTATAAAATGAAGAAAAAAAGCAGATTAAGATTAGGTTTAATAACAACATTGGCTAGTGGAACAATGGCTTTGCCATTAGTTTCATCTAGTTGTTGAGTATTTAATATTTTTAGGAAATATGACAAGGCGAATGAAAATGTTAAACCACCAGCACCAAAGCCAAAAGATAATAATGAGGACAATCTTTACGAAGACAGTAAATTTGATACAAAACTAATTGCTGAGGCTAGAACTGGTAAACAAAGACCTAAATTACGTAATTTTGATGCAATCAAGTTTGAATCTCATGATAAAACCGATTTTATCGGTAATTATTTAGGTATTGAGTGATTTGCTAAAATAGACTGAGGTGGTGATGCAGATGTATTAATTCCAGGCTTATATTATTATGGAATATACCTCAAAACATTACTAACTGGCAAACATTTAAATGATAATAATAAAAATGAATTATTTAATATTTCTGAGCGTAACAATCGATTAATAGAAGAGTTAAAATTTTTCGATAGTCCAATTAATGAAAAAACTAAAATAACATTAGAACAAGCATATCGTGCTGCTGCAGATGAAATTAGTTCTGAAGAAGGATTAAAAGCCTTTTATGGAGAAAACGGCCCAGATAAAACTGGTATGTCCTTGGCGTGGTATTTTAATGAAAGGGTGTCTGATTCAATTACCTTTATAGAGAAGGATTGGCCTGCCGGAACAACCGAAAATGAGTTAGTTGATTTTGTTAAGAAAAACACGATAAGATTTTATGATAGACTTAACAATGTTAATGGTTGAAGTATTAAATGAACTAAACATATTAAATCATTAAATAATCGACAAAAAATGGTAATTGATGATGCTTGTAAAGAACTTCATCAAGCAACAAAAATAGCATCTGCTTTAGGAGCATTTGAAATAATATGCGATCCAACAAAAACAGTGATTTATCAGTTAGATTTATATAAAAGTTTAATTGCTTTTTTCAATAATATTTATGCACTTAAACTTGATAAGACTAAACCGATTGAAGAACAATTAAAAACGGAAGAAATTAAAAATTCGCTACGTGATGCAATAAATAATGCAACAACATCATTGCAAGAAATGATGCCAGCTGGTTGAAATAAGCCAAATAAAATTCTCACAAAGCATGAATATGTTGCCTTTCAATATAGAAGGACAGATGACGATACTGACAAGGAAATGCAATATGATAAAAAATACCCAACAGCCAACTATTATCTATTTAATGCTGATAATCCTGATTGGGACCACTCACAAGCTAGGTTTGTTACCGATGTCTGCTACTATCTCAAAGAATTCTTATTACCACTTGCTGCCTTAATCAAGCTAGAAATTAGTGATGGATTCTGAGGAGATATCAACTTCTTATACGACAAAGCTAAATTATGTGCACAAACTAATGTTACATTCAATGCAAAAGATAAACCAATTAACTTCGAAAACTTATCAGAAGAGGAATGTAAATTAACTCTTAAAAAACACTTTGAAAAAATTAATAAAGAATACGAAATTTTCGGTAAATTAAACAATGATGAATATAAAACATTATTTAAAAACCTTGAAGAAAAACCGAAAAGCAAAAACGAATAATTTTTTACTTATTTATCTATAAAATAGATAAATAAAAAATACAAAATAAATATTTAGTTTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGTATTTTTTGCTTTTCAAAGGAAAGGAAAATATGAATATTACTACAACAATGTTTCGCAATGTAGATACAAGTACAACAGCTGAAACAACATTTAAAAATATACAACATGCATTGATAGGCCAAGCTATTACTTGGTCAAGTATTATACTTGGTTCCTTATTTGCCTTAGCTGTTATTATTACTATTATAATTGTTAGTCTTAAGGCAACTCTTGGCAAAAGAGATGATTCTTACCAAGAAAAATTAAAAGTTGTATTAAAAAGCTGTTTAATTGCAACTGCTGTACTTCTTGCGCTTCTTATTCTAACACCATCAATTTTAAAAATAATTCAAACTAATTATGGTGGCGAAGCACTTAAAGGATAATAAAATATGCTGAAAAGAATAGGATTTTTCTTTTTTAAAATTGGTTGATTTCCTTTTGTTGCTTTTCCATTCACAATAATTAATAGTTTAGGAAAGTTGATTAACTTTTTATCATTTGATTTTATATCATTATTAATTTTCGGTAAATCAATAGATGATATTAAATCAATAGATGATATAAAAAGCATACCAATGGCTTTTGCAGGGCTAGCACTTTTTGCTTTTGTTATGATTGCAATAATCCTTGGTATTGCTTGTTTTAAAGTCTTTTTTGGTGGTGGCGGAGAAGACAAAATGCAAAAGATGAAGCAGAGTTTCAAAGCGGCATTACCACATATGGCTTTTATAATCTTAATTCCAATTGTCGTTTGGGTCCTTGCATTTTTTGCAAAATTTGCATTTAATATGCTAAATCTTGCTTTAGGGGTAGACCCAGCTGCAGGTTTAGGGGAAATAATTTATAAAGCGATAAATCAACCAGAGGCAAGATTTCCAAATCCAACAGAACCAACATATGGCCAATACTCTGCTTCACAAAATGAACTTAATAGAAATAGCGTTGTATTAATGATAAATGCATTAGTACTGATTTGGTCAATGATAGTTATTGCAAATATTGCTATAAATATATCAATCAACTCATATCAAGCATTCTTTTTATTTATACAATCGCCATTAATAGCAGTAGTCTCAATTGAAGATGGTGGCAAGAAGCTTAATATATGGAAGCAACAATTTGTGTCCAAGATTCTTTCCGTTATGGTCTTGAATTTATCATTGTCAGTACTGGTTATGTTTTTTTCAGCTGCCTCAAAAGTTATAATGCCTATGGCTAACGCAACTAAAGTATGACCTTGATTAATAGCACTTATAGTTTTTGTTGCCGCATTATATTCGACTAAATCACTATTAAAAATAGTTTGTTCAATCTTTGGAGTAGAAATGACTAACTTTGTTTCAAACGCAGTTAATAAAGTTAAAAGCCTACACCCAGTACAAAAATTGAAGAACAAACTCAAAAAGAAAAATGCTGGTGCTGATGGAGGAGGAGATTCTGCTGATAATGAAAGTGAAGGTTCCTCTGATGGAAAAAGCAATGATGAAAATGCAAATACGAGTGATGCAACAGCTTATTCTGTTAAAGACGAGACCAATAAGAAAGCAATAAGCACTGAGAAAATTCCGCCAGCAGATATCCAGAAAACCAAAGAAAATATCGAAGGCGTAGCAGGAATAAGGGGAATACCTGATCTTTCAATTTATAAAAATGGAGATATTCAACAAAAATTAAGCGATATTTCTCTTTCATCAAAAGATATAGTGAAAAATCTTGCTGAAATGAAAAAAGCATCCCAAGAACTTGTAAAACATCAAGAAATAGATAACTATAAAGATAGCATAAAGGAGACAAAAAAACATGAATCAAAATAAGAAAATAAGCTCATTAAAGCTTAATATTGTAAAGAATTTAAACCTTTATGATATTTTGTTGTTATTTGCAATAATAGGCTTTTCAGCAGCATTAGGATTTGCTAATAAACTTAACACTTGAGTTCAAGTTGTAATTTTTGCCGTATCATTCTTATCGTTAATTTGATTGATAATACCAAGTAAAAAACACAATTGCAGGCTCTATACGTTAATAGTTAGAATATTTAAATATAAATCCTCTACGAAGAAATATAAAAAGAATACACCAAATGATACAAAACTACTTGTACCATTTAAGAGAATAATTGAAAGTAAATATGTTGAAACATTATCATCAGCTGGAACAAGTATGTTTACTGTGTTTAAATTTAATGGTTTTAATATTTACTCAAACAATAGAGATGATAAATACGCATTTTTTAATCAATTAATTAATGTATTTAACTCAATTGAAACCAAATTCACATTTGTTAAAAAGCCAGCTTCTATGAATATAGAAGATAACTTAAAATACAATGTTGATAAGTATTTTGAAAGCAAAATGTTACAAAGCAACGAACTTGTTCAAAACCATTTTATTTACAATACACATGACTTTGAACAACTTAAAAAAATAGAAAACATTGATATTTATTATCTTGTTATTTATGGTAAGAATATAAATGAAATTAAACAACTAGAAAATGAGTTTATCAAGTCATTTGAAAATACCAAAATACCTTTATCAAATGTCACCGGCTATGAACTATTAGACTTTACAAATATGATTTACAATGTATCATTATCACAAAAACAAAAACAAAATTTCTTTGACGAAACACTTGATGAAGAGCAAACTATGATATTAGATGCTAAAATGAAAGGCAAAGAACTTTCAGACAAGGAATTAAAGAAATTACGTAAAGATAAAGAATATAAGCTTATGAACTTGCTTGCACCTGAAACAATAGAGTTCGGTTCAAAGCATATAACAATTAATAAAGGAGAAAATGAAAAATATTTATCATTTCAAACCTTATATGATTTTAGTTCTTTAAAAATTAATGAAGGTTGAGCATGTGACTTATTTAGCAGTGAATCGCTTATTGTATGGAATTTAAAACCAATTAATGAAGCAACTAAACAAAAGATACTAGATAAAGCTATGCAATTAAATAATGCTAATGCAGACACAAAAAGTAATTTTAAAATTCAAAAAATTAGAGTTGAACAAGAAGTACTGGACGAACTAGTTAATTTTATTAACGTTCAAAAAATGAACTTATTTGATTCAAATTTAATTTTTGTTAATCAAAGTAAAGATTTGAAAACATTACACGAAATTGAAGTTCGGAATGGAATAAACGCTAAAAAAGCATTTGCCAATGTTTATTTAAATCACTATTTACAAAAAGAAGCTTTACAAAACTCAATATTTATCAAAGAAGACTTTTTGAGAAATGGCATTGAAATGAGCTCGCGTAATATTGTTCATGGTTGACCATGAGTATCAAACTCATTGAATGATGGTAATAACTTTCTATTAGGATTTACAGCCAACTCTTATAGTCCTATTATCTTTGACTTATTTAAGAAAACAAAGGACAGAACATCGTTTTCAATGATTTTATGAGGTGTGCCTGGTGCTGGTAAGTCAGCTGCTACATCTAAATTTGCATTACATCAATTTGTTAACGGTAGTGAAGTCATAATTATTGACCCACAACGTGAATATAAGGATTTATGCCATAAATTAAATGGAAACTGAATTGAAATGGGCAAAGGACTTGAAACCAAAATAAATCCTTTACAAGTTAACTTTGAAAGTGAAAAGGATAATGTTGCTGTTACAAACGATATGTTAATAACTAACCATATTAAGAAAACTAAAGAATTCTTTTCATTATTGTATCCTGACTTAGATGAAAAGGATTTAAGAGTTGTGACTTTCACACTATCAGAGTTTTATGCTAAGCAAAAATTCAAACCTATTGAAAAGATGAAAAATAATGAATTCCCAACAATGATTGATTATGTTAATTTCTTAGGTAAATATAAATTTAGCGATAAGAAGACAGAGTTATATTACAAAGATTCAATTAATAAACTATTTATAATCTTTAATCAAGATTTCTTAAATAATGGAATATTTAGTGCATTATATACTGGACATACAAATATTGATTTTGATAATTCATTTACTGTTATTGATACATATAACTTAATTAATAATATTTCTTCAAACGAAGGACAAGCATCATTATTCTTGATTCTTTCACACGTTAGAAATAGAATATCAAACAACTTCTTTATTAACAAAACTAAGAAGATTTGTTTAATTGTTGATGAAGCACATAGATTTATTAATGCTAAGAATATGTCTTCATTAGAATTCTTATTTGACACAGTTAAAACAATTCGTAAATACAACGGTTCTGTTATTTTAACTACACAAAACCCAAGTGACTTTGAATTAAGTGAAGACGTTATGAACCTTACAAAAGGTATGGTTGAAAATATACAATATACACTTATTTTAAGACAAAATCAAAATGGTGTTAATGCTATTAGTCAAATGTATAAATCTAAAAATGAGTTAACTTATTGAGAAAAAAACTTTCTCTCATCAGCGCCAATTGGTTGTGGAATGTTTTCAATCGACAACAAAACAAGATTTCAAATTTCACTACATTACAATGCACTAGAAAAGGAAATTATCTTTAAGTCAGGTGATTTAAGTAATATATAAAAATTTACTTATTTATCTATAAAACAGATAAATTATAAAAAGGAGGAATATGAAAAAACACAAAAAAATATGTCTTTTAGCAGTGCTTAGTGGGTTTGTACCATTAAGTACTATTTTCATTTCAAACTCTGTTAATACACAAAATAATAATGTTAATAAAGAAACTAATATAACAAAGAAAATCACTGTTAATTATTGAAACGATGAAGAATTAAAGGAGAATTCTCCTTTTGTTGATAAAGAAGGAAAATCACTCCAATGAGATGTTGTTTTTAGTTTTATTGAAAGCAAAGGTTTAAAAAGAAATCAATCAATTACATTATATTCAACCACAGGCTTTAAAGACAATTTAAATTTAGATATTAAACTAGAACACTATAAAAATAACAAAAGTTTAAAACAAGTTTGAGATGATTATTTTAAAAATTTACAACTAAACACATTTTTTGATAAAAATAAATTAAATTTAAAAAATGATGTTGAATATAACTTTGATTTTGAAATTAAAGATGATAAGTATATTGATATTATCTTAAATGAATTTAACTCAAGTAATAACCAATATAAGAAATATTATTTAATTAAAAATCAATTAATTAATTGAAATTGACCAAGTGACTTTAAAAACTTATCTTGAAAAGAAAGAATTTATTTCGACGCTGGATTTAACTATAAAACCGAAAATGGCAAAATTGTTTTTAATAAAACTAATCTAAAAATTACTAATAATAGCACACAAAATATAGAGTATATACCAGATAATATTTATATTGCCTCACCTGATGATTTAAATGAAACATTTGAAATTTCATTTAATGGTTCTACCATTTCAGGTCTTAACACAATTCAAGCTGGTAATGGAATATTGCACTTCGATTTAGCAGCCGCTAAAAACAATAAAAATAACAAAAATGATAGCTTAGAATTAAAAATTAAGTATAGAAGTAAATCTAATTCATCAGAAGAAACTATTACATTTAAATCTAATAATTCCTCAAAATATACTGATATAAATTTATCATTTCCTGGTTGAGATAAGAACTCAGATTTAGCAAAAAAATTAACTAGTCCTAAAATTCTTGATAAAGAAGGTAATGAGACAGACGAAGTTAATAAAAATTATGATTCTCTAGTTAATATTGAAACAGGATTAAAAAGTGAATTGGTGTGGGTGGATTTAAAAAATATATATCCAGAACTTGATATTACCACATTCTATCATCCTTTATGAGATAAATTGAAAAGGGAAGATATTAAGTTTGACCAAGATTACATAAAGAAAAAAATTAATACGCTTTTTGAATTATTATTTTGTAATACTCCTACACCTTACTCAGACAAAAGCTCTTTACACACCCCAGGAATTGATAGTGTATGGGAATATATAATAAAAAAAGGTTTTGAGAATAAAAAAGCATTTGAAAATTTTTTGCCTTTATATAACAATTTTTATAATAGTCTTTTTTCTAATGGGACAAAAGTAGACAAATATTTATCTGATAATTCGTTTAATATTCATTGAAACAGCGATGATTATTTACGAAAATTTCAATATATTTATTTAAAAATCGAGGAACTTAAAAAATTACTTAAAAATTTATTTAATAATCTTGACGAAAAATCTAAACATGGCGATATGTATTCTTTTCTTTCATCTTGTTTCAGACATGAATATAAACCTTTTTTTATAAGTATTTCTGGATCTTCGCAAGGTGTATATGTTAGTTTTGATTCTTACGATTATTCAGAATTCTTGCATGAATTATTGCATGAATTATCAAAATATATTCTTTACTATTATTATTATAGAATTGTTGATTTTGAATATTACGATAATAAAAAATTCTTTGAGGATAGTCTTGGTTTGCACCCAGAGCTATATAAAGAAATGGAGAAAGAAGGTGGCCCTCTTTACCATATATTTTTCTTAAAAGAATTTATAAATTTTGGAATTAAATCCTTTGAAATGGAAGACTACAAAAAAGTTAAAAAATTTTGAGATAAAAATATGAAAGATTTTCAAGATAAAAATAAAGAAATTTTAAAAGCAAATCATGAAGTATGAAAAGAAATAGGCTGATGAAAAACTGAATTACCTGTTTTTACTTTTAAAAATAGTTTAATAGATACAACAAATGAAAGCAATATATCAATTCATATGCAGAAGACTGAAAAATGAATACAAAAAACCTTTAAAAATCTTACATACATAGATAATCAACTCACCCCTATTTCTGGCTTCCCAGCAGAATTCATTAAAACAAGTGCAATAAAAGTAAATGCTAATACAATAGTTGTAAAAGCAAAATATGATAATGGGAAGCTTTCAGCAGATGCCGACTTCACCAAAGGTTTTAGTAAAACAGATTTTGAAATCAGAGAGAATGGATTTTACTTGCTTTATGATATTTCTAAATCTAATTTAAGTAAATTTAAATTAGTATGAATAGATAATAAGTTTGAAATTGAAGCTAATAAAGGAAGATTAAGTGAAATAGAAGGCCTACCAAGTTTTGTTCATAAATTCTGAGGATCAAATAACTTTGAAATATCAGAAGAATTTAAAAAATACTTAATTAAAGTTGGCGTTATTAACACAAGTAATTTTAAAGAAGAAGATTTATTAAATTCGAATAAATATAATTATGATAAGTTATTTCCTCTTTATCAAGACTTCTTGTTTTATAGATATAAGTTTGCTACATTTAATATAAATATAAAACTAGATATTCTAAAACTAAAGAATGATTTTAAACCTGATAAAAATAAGAGTGCTAATGAAAAACTTCTCAATCATTTAGAAGCTTCTAATTTTGATGAACAAGATTTTTTAAATAAATATGTTAAAGCGACATCTAAAATAGATAAAAATGATCTAAAAATTAAAAATAAATGAAAAAGTGATAATGGTAGCATTTATATCTATATTGACCATAAAGATAAAAACTATAACCTAAATAACAAAGTCTTTAGAATTGATTTATCTAAAATAGCATTATTGTTAGAATTTAACGATGATGTTGAAACTATAAACAACAATAAGACAAAGACAATAAACGAATTTGAAGATATTATAAAACCATTAACTGTCGACAAATATGTTAAATCTCTTAAAAATTCAGAAACGGGTGAAGATATCAAAGATATTAATAAAATAATTAAGTATAGATATATTATAGATACCCATAAAAAACTAAAAATTAATATTAGTATGTTAAATAATGAATATTATATAAGTAATTTCTCAGAAAAAGAGTATGACGTTGATTTAGGAGCATTTGACGAAGCATATTTATCTGACTTTGAACCACCTGTTATTAATCTTTATGGCCGGAATACGATTGATTTAATTAAAAATTATCTTAAAAACACAAGCGAGTATGGTGTCTTACAAAGTTATTTAAATGAATATAATCGCAAAAAACTATCTGAAAACAACAAACTTTTTTCAAACAAAAAAACTAAATGAAAAAATATTATGATGTCCGACATAATTATCGAATATGACCAAGATGAATTAATAAAAGTTTTAGAACCTAGAAAAGATAGTAAATATTGAGAAGATGACAAAAACAAAGCTAAATTAAAGTTCAAAATCAAAGGCAGAAAGATTAGCGATGCCATTTATATCAAACTTATTAATGATGCACCACCAGGTAAATTTAAATGGCCTGATGTAAAAAAAATAGAAATAAGTGTTGATACGACTGATATGGACAAAAATTTTGAAAAAATTAAGAAAGAAGCAAAAGAACAAATTACATATAAAATTAAAGAAGCTTTTGGCCCAAATTCAAAGCTTGTACAAAATAATTTATATGATTTTTTATGAACAGATGAATTTTTTAAAAACCTAGAATATAAAATAGGTACAAATTTAATTCTTAAAGGCTTACATTACACGTTGCAAGATGATAATGGCGTTGACTTTCAGATAGTTGTTAAAAATTTAAAAGCAACTAAACCATATGACTTAAATAAACTAGAAATTGATACTGTTACAACTAGTGAAGAAAACAAGGAAAAACTTATTGATTCTGTTTATAAAATAATAGAAAAAAATACTCTTATTCCAAAAGAAGAATTAAAAAACAAGACAGAATTAATTGTTGAAGATGGTAATTGAAATTTCTTTTTTATTGAAGCAGGCACACATACTAAAAATGTCAAAATAATACCTAAGAAAGGTTTTAATGACTTTAGTGGTAATAAAAACTTCAATTTATCTAATAAATATACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAATCTAAAAACAAGAAAAAATTACTTTCATGACTAATTCCAGTAATTATTTTAAGTGTTGTTGGAATTATTGCAACAATTATTGCAATATGGTATAGAAAGAAGAAAAAACGTTTATAGATTAAGCCTATAGGCTTCTTTTTCTATTTCTATGCTTTTACTAAAAATACTATAAAACAGGATTATAATAAAAATATGAATTTACAAGATAAACAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAGTTCATTGCTGTTAACTTAATCAAAAATATGATTAATCAATTAATTGATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTGTAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAGGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCTAGACTTGCAAGCAAATACAATGTAAGCAAAACTCAAATATACAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAGAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACCTTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACAATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCCAGCAAATGTTTATCAAAAACAATTAAAGAAGCAATAACAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGAATTCAAACACTAGCAAAACAATTAAAAGGCAATTTTATTTTGTGTAAATTTCACTTTTTAAGAAAATTTAATGATTTTATTTTTAAAAAGAGAAATCGTGGATTTAACAATTTTATAGATAGCATTCAAAATTTAAGTGGGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGTGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAGTATTTATATTAATATTAGTATAGAAAGGCAATTAAATTTGCTATTTTTTCCATACAATTTTCTTTGTAATTTACATTTTTTAATATAAAATATAGTTGATAGAAAAAGGAAATACCGTTTTCTCTTTTAAATTTCTAACTTATCCCATTAATAATTTACATCACCGAAAATGATATCATCTTTATAGATGAAATTCATAGCATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAGTGAATTAATTTTATTTTATTAATATTAAAATTTATATTTGATATATAATTGAAAAGTTATGTGATATAAACTTAAGCCTTTCATGAGAAAAATCTTTAGTTGAAGCAACTGAAAAAGATGAGTTGTTTTAAGTATTACATTAATTTCCACAATCATTGCTATCGTTTGTGGAAGTCTTTTCTATATTTCTAAGAAGCCAAACTTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGATACAAGGCTACTGAATATATTACCAAAAATAATAAAGATCCAAAAAAAAGAAAAATATTGATGTGACTTAATCTAGATGCTTTAGTTAAATTTGCAAAAACTGAATATGCATCAGATTGAAAAGAAGCGGGAGAAAATCCATATAATTTTGTTCATATTGGTAATAAAGCGTTAAATGATAAAGGAAAACCAAACGCTTTAAAAGAATATGAGTTTAAAGGAACAAACTACTTATTAAGTGAAGCTGGTCTTAGGGAACCATTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGACTTCTTGGAATTAGACAAAAGAAAATATTAGCCCCTAAAAAAGAAAATAAATTTAACAAATTTGATTTTGTTAAAAATTCAAAATGATTTGGAATTGGTTCATTAATATTTATTGTTGCAGGAATTATTGTTTTTGCAGTATTTGCTGGAATTAATAAAGATTTCTGAGCAGGATTCAACAGATCTATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGAGGAATTGATCCGAATAAAATTACATTCCAAATAGTGCCTATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGAACTTATTCAATTGCAGCAGTATTTGGCTTATTACATGATGTTTTAATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGACTTTGAATTAAACTTGAAACTAAACGTCAACTTTGTATCAAAAAACGTGCAGATAATAGCTACTGAGTTTTATCTGGATCAGAAGAACAATTATTCCAAGGTATTAATGATTTTACAGCTTAAAAAGAGACAGGCTAAAGCCTGTTTTTTATTTGCCTTCTTATAAAAAATGCTTTTATTTTTATAATTTTTTTATAAAATTATAAGGCACACCAATAAGGAGAAATATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGATTTTTAAATCAAAAAAGCCAAATACCTCCTATTTATAGTGCAAAAAAGATAAATGGTGTTCGTAGTTATAATTTAGCTAGAAATGGCCAAAAGGTTGAGTTAAACACTCAAAAAATAGAAGTTTTTAACTCAAAAATTTTAAATTTTGATTATCAAAAACAAGAGTTAACTATTGAGTTAGAAGTTTCTTTTGGAACATATATTCGTTCCTTAGCAAATGATGTTGGAATAGCATTCAATACTTATTCCTACATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTGATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAATTATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGATATTCACCAAAAATGAATATTAATACATGATTCTTATCACCTCATCAAGCCAAAATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGAGCAACTGGTGTTTTTGTTAGTCCTAAAGGAATAACCAAATCACACACTTTAGATTGATTAGCTAAATATTTAAAACTAGATTCTAAAAAGAATTTAATTGCTTTTGGCGACAGTTCAAATGACTATGAAATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAATTTAATTTATAATTGAAATATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAGTAAAAAAACAAATTACTTTTATTTTGCATTACTTTTTGCATCTAAAATAAATATTTGGTTTTTTATTTTTATTTATTATAATAATAAATATTTATTTAAGAGGAGAACTATGGCTGTTGGAATTATTGCTGAATATAATCCATTTCACAATGGACATATTAAACAAATAAACTGAGTTAAAGAACATTTTCCAGGTGAAAAAATTGTTGTTGTTATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGCGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGAAAATTTCAAAAAATAATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTTGAAGCATGTACTTCAAAAAGATATACAGCTTCAAGAATCCAAAGAATAATAGCTTGAATTTTAAATAGAAAGTGAAAGAAAACTTCAAATCGAAAAGTTAAAAAATTATTAAAATTCATAAAAAAGAAGAAACACAAAATATTGAATCAAAGAGTAGATAAAATTAATTTTTCTTTTTTATTGCATTTATTTTTTTAATTAGGAAATTCTAATAAAATTAAAGTATATTCAAGTAGAAGGAGTTTTATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGAAAAAACATCAAAGAAATATTTGATGCAGATTTTTTATTAAAATATCGTACTTTGCAAAAAGAAGGGCTTAACTCTATAAACTGTTTAGCTCAAGATTTTATTAATGAATGAGAAACAAATCAAAAAAGATTTGAAACAAGTGATTCGCTATTACCTGTTGATTTATTTAAAAATATTTATTCATATTTTGAAGAAAAACAAGATTTACAAATTCAAATAATTGATAACTTACATAAAGATATTAATCAATTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGAGATCATCAAAAAGAAAATGAAATTTTACTTAAAAATTATCAAGAAGAATTCAAATCTGAAATCAAAAATTATGAATTTGAAATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGACTTGCAAAAAGTAATAAGAATGAAAAAGTTGAAAATACAAATTATGCAAATAATTTACAAAATAAATTAAAAGATAAAATTTTAACCTTAAAAGCATTAAATATAGAAATTAGCCAATTACAAGAAGACTTAAAAACTGTTAAAGAATTTATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAATTGAGCCAAATCACTATTTATATGGAACTAGAAATCAAGTGCTCAAGTGAATTGAAAAATATAATTTAGTTAATCCGGTTTTAGCTCAAGAATTAGAATATCAAAAGATAGCTAAAAACACAAAAGATTCACCTTTTGATGGACTTAATGAAATACCTGAAGGTGTTGATTACACTCAAGTTATGGATATTAGTATGTTCACTTCTGAAATTAAAGTTTCAAAAACAACTTTCGATTTAAACAAAAAAAGTAAGGAAAAATAATGCTTAAGCTTTTTTTTACAAGTCAGCATTATTTTCTTTCAAATTTTTTTAATTTTGCTCGTTAGCAAATTCTTTGTATTTACTATCAACATCTTGAACTTTGTTATGAATATCTTCATACCATTTTTCAAATTCTTTACGATATATTGATGAAATAAAAATATGACCATTGCATTTTTTTGATTAATAATATCAATTTTGTTTATATTTTCTAAAAATTAATAATTAGCTCATTTTTAAAAAGTATGAAAATTAATTCTAAATTTTTTGAATTCATTTTTTTCTAGATCGCTTTTGCTATTAATAGTTTTTAGAAATGTTTTAAGATAACTTTTTTCCTTGTTATATTCTTCTTTAACAACTAAAATTGGCTTACAACTAGCAGCCAAAACTGGAGTTAATAAAGCAAGTGAACTAATTGTATCTAAAATCAATTTTCTTATTCTCTTCATACTTCTATCATAGCAAATATAATAATAAAGGGAAATTTGATATTATCAAATGCAAAAAATACTTGTTATAATTAATAAATCACAATAAGGAGAATTATGCCAATTTGAGGTCAAATATTATTAGTACTTTTTTCATCAATATTTTTATCAACAATTTTTGTTGTTTTAACATATAAATTCTTTTTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGAATTGAATCAAATAAAGGATATGAAGAAGAATTTACTTTTTATAATAAAGCATATGAATATCAAATCGATTTTTTTGATTTTGATTTAAATAGTGCAAAATATATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAGCCCTTACTTTTTTAAATAAAAATCTCACAATTCTTGTGTCATTTTGTCATCACAATTTTCATCATTACCATTTTTTAAAATATATTCATATCAATTTTTATGCAATTGTTGAAAACTTTGAAGTAATCTTGTTGCATATTGATATATTTTTCTATTTCTTAGTGATACTGGACCAGGTTTTAAAGTATCTACTTGAACAATATATTTAGAAAAATTCACTAAACTTTCTCTTAATTCTTCATATTTTTCTTCTTGAATATCTTTTTTATTTTCAAATTCTTTGACACATTCTTTTGATTTCACTATCTCATTATTGAATTTATTTTCAATAGCTTTTAAATTGCAGCCACTTGCTGAAATAGTCATTGAAATTGGAATAGCAAAAGAAGAAACAATTAAAATATTTTTGATTTTTTTTCTTTGCATTTCTAACCTTTTTTTGTTACGAAATTAAGTGTTTTTATTATTTATTAAGATAATATTATAACAAATTTCAACATAATGAAAAGAGTTGTGAAAAACTTATGAGAATTTTTCAAAAATTATCTTAAACATTTAAATTTGTATATTAATAAAATATAATTTTCTCGTGATAAATAATATAGCTCAATATACCATAGTTATCCATAACTATACATTCTAAAATTTATTTTACTTATATTTATTTTTGTTTTATTTGTTATTTAGCAATATTTTTTATCATTAAAATACATTTTATTTGAAAGGAAGTCAAAATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATGTAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGATTAATTCCTATTGCTAAAAGCTCAATTAACTTAGGCGCTAATGGTCCTGTTCTTTCATACGTAATTGAAAGTATGCTTGCAAAAGGGGCTAAAATTGATCCAAGTTGAAAAGATAAATGAGATAAAATCAAAAAATCAGGTTTAAAAGATAGAGCAGCCGTTATTGAAAACTGAGGTGCTGTTAAATCAGATGTTAGTGCATTAAAATTAAATGAGTTAACAATTAAAGAATCAACATTCTCAAATATTTACTCATTATTAGAATTTTGTGACAAAGCACAAAAAATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGAATTACAACAGTTGTTGAAGAAGATGGCGAAAGAAAAATAAGTTACAAATCATTTGTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGGTATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGAAATGAAAACAATAATAAATTTGCCCAAATTGTAAAACATGATGATGGAACATATCGTATTGGTGGTCACTTAAATAAAATTAAAAAAGACACTGAAAAACTTGAAAATTATGAATGAAAAGCAAAAACCGCAGATATTTACAACAAATTAGCAGGTTATAGTCAAATTCCTACTGGAACTAAAAAAATTAGTTCACCAAGTGGAACAAATGTTTATGCTTTCATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGATTTGTAACATCAGGCGCAACTGAATTAGAATATAAAGATCAAGAAACTGGAAATGTAATTAAAAAAGTAATAAAACCTTCTTTAATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGAAGAAATGTTTATGGTAACAAAAGCCCAGGACAATATAAAGATCAAATAATTGACGCTGTAGTTGCTTCAAACAACAAATTATTTAATTAATTTTAATTAAAAAAACACAATAAATTAGATAGAAATATCTAGTTTATTTGTATAAACTTAAAAATTTATTTTTAAAAAAGGACTTATTATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGAAAAGAATATGATAAGTATGACATTTCAAAAATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGAGATCAAATGGCAAAAGAAGGTTTAAATTACTATGATGGCGAAATCGTAAGTTGAAATGATGGAGATACTCCTAAAGTCAAATATTATGATCCAAAGAAAAAAGAGATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGAGGCGAAAAAAATGGACACAAGTATGTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAGGGCTTTAAGATTAAATTAATATTATTAAATTAAAGGAAAAAATATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGACAAAACAAAGTTTTAGATAGAAAAACAGAAGCTATCATTAATATTAGAAATATTTATCTTAAATCATTAGGTGCTAGTGATTTAGAAATTAAGCAAATTAATGAAGCTTGAAAAATTTACAAATCAATTTATAGAGAAACACAATCAGAATTAGCTAATTATCGTGTTGAATTACGTGAAAAATTTGATAAAGCGTTTACTGATTATAAAATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAATAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGAATTAGAGATATTCAACAAAAATTAGGTATTACTACAGTATTCGTTACACACGACCAAGAAGAAGCTATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAACTTTTGAGCAAAATTAGCTATCAAGTTTTCTTGATTAACTACTTTTAGTGCTCAAAAGCAAGCTAATAGAAAAGAAGAAGCTCTAGCTGAATCAGTAGTTGAAAAGCGTACTCCTGCTTACAAACCATTGATTTTGTTAGTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGAGCAAGAGGTTTTTGACAAACAATATTCTTCTTACCTTATATAACAAATATTGTTGCTGTGTCTTTATCATTTATTCAATTTTTTGAAAATAATGGTTTATTCAACACAATTTTCAATTTAGGAGAAATTCCTTGATTAAATGATAAATCACCAAAAGCAATTATTTCTTTCTATCCATTCCTTGCAATGGTTGTTCAAGGTATTTGATCAGGGCTAGCATTTAATGTTTTAATTTTTACAACAGCAATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGAAACAAAATTAAAGATTCAGAAGTGATGATCAAATATCAAAATAAGAAAAAACAAAGACAAAACAGCGCAACAAGTTAGAGAAGTCTCTGCTTTTGGAATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGAACAAACTATTCAACCGCTGCTAATACAAAAGCTAATGGTAATAGTGATTATTGATCAGCATTTGCATTAACTTTTGCCAATGTTATTTTCTCGATTATAGTTAAAATATTTATTACATTATTTGCAGGTTATGCCTTCTCGCTTAAAAAGTGAAGAGGTAAAAACATATTATGAGCTATTTTAATGGGATTATTAATTTTACCTGAAGTTGCTCTTCTTTCAGGACAATATAAAGTTGTTACTGAATGAAACAATGCAATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGAAACTCATACTTGTGACCATCTCTTGCTCTTGGAAGCGACTACTTCATTATTTCTACATGATTATTCCATGTTGGTCGTGATGAAAGTAGTGGTGTTCCTATTATTCTTCAAAGTATTAAAATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAGGAAGTGAAATTATGAAAAAAGCAAATATAAAAACCAAAACAATAATTTGATTAATACTCGCTATTATTGCTTTTATACTTTTAATCATATTTTCAGTTGTATTGCATAATACTATTCAAGTTTTAAAATTATCTGAATTTATTAGTTTCGACAAAGACTATCTACATGAAGCTATGTCACAATATTCATTTGCAATTGCAATAATTACATTTAGCTCAATCATAATTCTAATAGGTACTTATATTTCATATGCAGGATTCAAAAGTTGAAAATATAATGCAACTATTTAAGGAGAATAATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGATTACAAAAAAATGCTAAAGATAAAACAAGTAATCAATTAGAATTGGCTTACAACTTTTTTGCTCCACATCGTGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGAAAAATTTTTAGCTTATCAAACTTTTTTGATAACTTAAGAAAAAAATTCGACCCAACTAACCCAGTCGATGAAGAATTCACAAAAGATAAATTAATTACATATGATGCATTTCCATCTGATTGAACATTTGATTTACCTAACACTGTTACAAAAGATGATGTAGATAAAATGTCTATTTACTTAGAAAATGGTGTTAAAGAACTTAAATTTACATTGACAAAAATAAAACAATATTCTAATATCAAATAATCAAAAATAAATTAGGCCTTGCGCCTAATTTTTTATTTAGCCCTGTTTTATAGTGCTTTTGGTAAAAAAAGCAAACTTATTTATTCTTCTTCTTTATCATCATTTTCATCACTTAAATCTTCAATCATAGGCATATTCATTGCAACTTTATCAATGTCATAATCTTCTTTTATAATACCTTTTTTTCGTGCACTATCAATAGAATTTTGAATTCTTTTCTTTACTCTATCGACTATTTTTAATGCTCTATTTAATTCAACTCCTGTTTTATCATTTTTCTTGATACTTTGTTCAATTTTGGTATAAATATTATCTTCACTTTGACGAATAAATAAGAATGCATCTAAAAGAATGTCAATATTTTGTTTTATGTTAATTCCATAATTTGCATAAGCAATGGTTTGAATAATCATCAAAATTGTACTTGGTCCAGCCAACATAATATCATGATCTTTATTCAATGCTGAAACCAATTCATTGTCACTAACTGCTAAAGCAAAAATAGCTTCAGATGGTAGATACATAATTGCTTGCTTGGTAGTTACATTTTTAATAATGTATTTACTTTTAATACTTTTAGCTTGTTCGAGAATAACTTTTCTAAAGTTAATTTGAGCTTTAATTCTTTCTTCTTTAGAGTCTGCATCTTGATATGCATTAAAAGAATCAAGTGGAAACTTACAATCAATAGGCAAGTAACGATCATTTTGAGAGTTAGCTTCTCCATTAGGCATTTTAATAACAAAGTCAACTCTTTCGCTATTTGGGTTCATATCTTCAAAATCAACAGCAGCAGCTTCATCTAATCCTTTTTTAGCAATTTTCTTTAAATCAACTTGTGCAGCTCACAAGTCTTTTGATAAATGTTCATTCAAAATACTCTTTAAATTAAACTCACCATAATTACCGCGACTTTTTGTTCCATCAAAAGTTTTTTGTAATGAATTAACTGAATCTTGTAAGGTCTCAAAACGTACTAAATTCTTGTCAAGATTTTCCATGTTTTCTTTAATATTTTTAAAGTTTTCTTCCATCTTTTTAGCTACTTCATTTTCAAATTTTTCATCAATAGCACCTTTAATTATTTGCACTTCCTTAATTACTAAATCATCAAACGATTTCATTTTTTTGTCAATTTCTTCTCTTAAATCACTAAAGTTTTTATTAATCATGTTGAATGAATTGGCAATTTTTTCATCAACATTTTTATTGAATTTGACAAATTGACTAGACAAATCTTCAGTTATTTGATTTTTAATATTATTTGTCATTTTGTCTTGATCGATAACTTCTGTCTTATTTTTTTTATATAAGATGAAACTAACGACAATACTACTAATAGCTAAAATCATAGTCAATATTACTACAATTAGAATTCCTATTTCCATATTTTTTCTCCTTTAATTAATTGGTTTTTTAAATTATATAATTTTTAATTATATTTTGATTAAAATAACAACTTAAAAGCTCAAAAATTGCCACAATAAATATTATTTTTTTGTTTTTGCAAGACTCAAAAAGTTTTACAATTTAAAATTGTATTGATTTTAAAATCTAGAAAGAATTTATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGATTAAATAAACCAATTTGCTATGGTGCTTTAGATGACCATAATAAGTTGCTAGGTTTTGCTGAAGGTTATTTAGAAGAGTGAAATAATCGTTTTAGAATTACTAACATTTTAGTATTTGATGAAAATAGTAGACATAGTGGTATTGGTTCTAAATTAATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAGAAATTTTGCTATATAATAGAAATATAGTTTTTGGAGGTAAGATATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGAGTTTGTGCTATTTGTGATGCGCCTAATGTTCTTTATGAAAATCATTTTATTGATGATAAGGTTAAATACAGTTCATTTCCAATTATACCTTTAGTTGGCGGTAAAAATAGAAATGAAAAAATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAAAAAGGCAATAAAAAAATCAGCTTTTGAAAGTTGATTTTTTATTATTCTTCTTTTTCAATTTTATGCTTTTGAGCCTTTTTTATAAGCACAAAGAGCAAAATTTGAAGTTTCAACTTATCTCATCAAACAGTGTTCGAAATGTTTTCGAAATTGCAACACATTTGATATTCAATAACCAATTTACCTTTAACATCATCTCAGTAAAAAATCATTTGATGTTTCTTATTGTTAATTAATTTAAAAAGCATGTATTTATGATCATTTCATACTCGATATGCATTAAAAGCTGTAAATCATCTTTTGTAATTTAATGAGAAATATTTAATTAAATTTTCTAGTTTATCAAAAGAATATTTGTATGATTTTTTGCTAATTTTAATCTTGCCAACTCTTTCAGCTAATTGATTGTATTTGAATGAAAGTAAGTTGTTTCGATTTGTATAACTATTTAGCATTCAAATTAAACAAATGAAAAAGTAATAGTTATTAAATTGAGTTGAATAACGAGGATTAGCTATAAATTTGTTATCAATATAAGCAAATAAACAGCTTTCATTTTTTAATTGTTTATTTATATTTTTTAAATCATAGAAATAATATTTAACTTTATATTGTTTTAATAATTCTAATATTTGATAAGTAGTATTTTGTGGAACAACAACATAAGAATTACTAATATCAATTCCATTACGTTTGATTTCTTCTAAAAAGAAATCTAAATAAATTAATACTAGTTCATGAGAATTAAGTCATTTAAATTTGTCATTTAAACGCAAGCAAACATTTAAGTTGCTGTTGATATCAAAATGAAAAATATTTTGAATTTTGTTTTCACGATATCATGAGCTAGCTGCAAAAAATGTTAATCATTTTAGGTTTAAAAGCGAAATATTTTTATTGTTTGTAATGTATTTATTTTGATAATTATTTAATAGTTCTGTACCAATACTTTGACTAAATTTATTGTCACTAGCAAAGTAACTTAATGTTTTATATCTTTGTTTAACATTAACGAAAGCTTTAAGAATTTGTTCTTTTGAAGCTAATTCATCAATCAGTTCAAATTCATTAACTAGTTTAGGATCTTTGATGACAATTTCTTTATTTTGCCATTTGTTTAATTGATAATAATCATAAATTTTTTGTAAGTTTTCAGAGGTCAAAATTTCAAAGTTTTTATCAAATATTTGAATTGTAATATAGTTATCATTTTTAATATTTTTAAATTTGATTAAAAAGTCATATGAAGCTTTTTTATAGGCATATTCATCTAAAATCAAACTAATTGAAAAGTTATTGTGATACTTAAAAACATTATGATTATTTTTGATAAAATAAGAGCTAAAATATTGAATATTTTCATTATAAAATTCTTGACCTTCAAAGCTTAATAAAATGTTACTATTGCTTTTTAAAATAGTTTTATTTATAGCTTGCAATAGTCGCAAGATTGATGAGAGTTCTATTTTTTCTTTGTTATTTGTTTTAAATAAAAAGGTATCATTGCGAATTAAAAGATTATCGTTTGAAACGATAATTTGCTCTTTTAATTGTTGATTATTTGTTTGCATAAAATTACCTATTATTTATATTAAAGATTTTTGATTATTCTTCATTGTTTGAATCAATATCAATTTCATCGTTTGAGTTATCATCTAAGTTGATTTCTTGACTTTGTTCATAAATGCTTTGAACAACAGAAGTTGCAGCCATAGTTGCTTCTGGATCTGTTTTTTCATCAATTTTAATGATTTCAACAGCTTGAATTTCTTCACGCTTTTTAAGGTTAATTATTTTAACACCTTTAGCTGCCCGACCAGTTTGGCTAATTTGGCTAATAGCTGTTCTAATTGTTAAACCACTTGTTGTAATAATTAAAAGTTCATCTTGAGGATTTACGAATCTAGCAAAAACAATGTCGCCTGCTAATTTGCTATTAATTCCTTTAACACCTTTACCACCACGTTTAATTAATCTAAATAGTGAGTGGTGAGTAAGTTTTCCAAAACCTTTTTTACCAATAGTTAAGATTAATTCACCTTCTTGACTTGTTGAAGTGCTTGAAACTGTTTGATGGCTATCAAGTGTTATACCTTTAACACCAGTTGCAGTTCTACTAATTGGTCTAAAGTCAATAGCTTGGAATTTAACAATTCTTTCTTCATCATTTGCTAAAAGAATTAAGTCTTCATCAGTAATAATCATTGCTCTAATTAATTCATCGCCTTCACGTAAGTTGAAAGCATATTTACCATTAGCGTTAATTCTTTCGTATTCAGTTAATCTAGTTTTCTTGATAATACCATTTTTAGTTGCTGTAAATAAGTAGTGATCTTCATCGTAAGTGTCTACTGGAAGCATTGAAACAATTCTTTCACCTTCTTTAACACGAAGTGAAGGAATAATATTAATGAATGCTACACCTTTACTTTGACGAGATAATTCTGGAATTTCATGAGCTCTAACACGGTAGACACGAGCATGATTTGAGAATAATAGTAAGTCGGTGTGAGTTGTAGTTGTTAATATTGAGGCAATATCATCATCGTCATATGTTTTCATAGTACCAATACCAACTCCACCACGACGTTGAGTTTTGTATTCTTCTAAGTCCATTCTTTTTACAAAGCCTTTGGCGCTGGTTGTAATAACAATGTCACGTTGAGGAATCAAGTCTTCATCAGTAATAGTACCTTGAATTTCATAGTTAATTTGTGTGCGACGTTTGTCATTGTATTTTTCTTTAATTTCAGTTAATTCTTTAACAATTAATTTAATTAATTCACTATGGTTAGCCAAAATAAATTCAATTCTTTCAATTTCTTTTCTTAACTCTGCCATTTCTTCAAGCATGTTTTCAATAGCAAGTCCTGTAAGACGACCTAAACGCATGTCAACAATAGCTTTTGTTTGTTTTTCAGTTAAATTAAATCTTTTTGCTAAGTTAGCTTGAGCTTCACCATCTGTTTTAGATTTCTTAATAATTGCAATAACTTCATCAATATTTTCAACTGCAATTTTAAGACCTTCTAAAATGTGAAGACGGTCTTGAGCTTTAATTAAATCAAATTTTAATCTTCTAGTCACTACTTCTTCTTGGTGTCTTAAGTAAACTTCTAAAGCTTGTTTTAAGTTTAAAACTTTAGGTTCACCATCAACTAAAGCAACAAAGTTAACATTGTAGTTTACTTGTAAGTTTGTTTGACGATAAAGCTTGTTTAATAAAACATGAGGATTAACATTCTTTTTAACGTCAATAACAATTCTAATACCTTCACGGCTACTTTCATCACGTAAGTCGGCGATACCTTCGATTTGTTTATCTTTAACTAATTCAGCTATTTTTTCAATAATTGCAGTTTTCTTAACTTCATAAGGAATTTCAGTAACAATAATTTTGCTTTTACCATTTGAGTATTCTTCTATTTTAGCTATTGATCTGATTGGAATGGATCCCTTGCCTGTTAAGTATGCATCTTTAATACCTGCATTGCCTAAAATCATAGCTCCAGTAGGAAAGTCTGGACCAGGTAAAAATTCCATTAAGCCATAAATATCAATTTCTGGATTGTTAGCTAAAGCAATAGTTGCATCAATAGTTTCACCTAAGTTGTGAGGTGGAATACTTGTTGCCATACCAACAGCAATACCACTACCACCTGAAACTAAAAGGTTAGGGAATCTTGATGGTAATACTGAAGGTTCTTTTTCACTAGCATCATAGTTATCAACAAAATCTACTGTATCTTTTTTAATACCTTCAAGCATTTCAGCAGCTAATTTGCTCATTCTTGCTTCAGTATAACGCATCGCAGCAGCTTCATCACCATCAATAGATCCAAAGTTACCGTGACCATCAACTAAAGGATAACGCATACTAAAATCTTGCGCCATACGAACCATAGCTTCATAAACTGAACTGTCGCCATGTGGGTGGTACTTACCTAAAACGTCCCCGACAATACGAGCGCTTTTTCTATGTTGCGATCCAAATGTAATTCCTAATTCATTCATATCAAAAAGAATACGTCTATGTACTGGTTTAAGTCCATCACGAGCATCTGGTAAAGCACGAGAAACAATAACACTCATTGCATATTCTAAGAATGAGTTTTTCATTTCTTTGTGAACTTCAACTGGATGAATACCATCTTTAGGTGCATCTAAAAGTTGTGGTTGAACTACATAGTTGTCTTTATTTTGAGGAACTTCTTCTTCCTCCCCTTCAATAACTTTAGTTTCTTCTTTTTGTGGTTTAAAAGCAACAAAAAGCTCTTCATCAGGAATGTAATCTTCATCTAATTCTTCGATTGCATTTTCTTTTTCTTCTTCTTGATTAAGTTTGCTTTTGTATTCTTTTTCTTTATCTTGTTCTTTTGAACTCATAAAATACCTCCTTCAAACTTTTAATATATAAATATATTAAAATATTATAGCATTAAAATGCAGTTTTTTTAATAAATTTTTTATTTTTTTATTTTCATTTTTTATGGCTTTTTTACATTAAAATTTTAAAACTATATTTTTACTAAATTAGCTGTTTTATAGGCTTTTAATAGAAAAATCAATATAAATTAAGAGAAAAAATTAAAAGCTAATTATGTTTGCTTTAATTAAAATAAAATATAATTAAATAGTTTAAATTATAGAAAGGATTAAAAAATGGAAAATATAAACACAGAAAAACATAAAAAAATGGACTTCAAAAGTTGATCAAAATTGAGAAAATCAACATTTATTGTGGGTATTATTGTTCTAGCAATTTGTGTGTTAACTTCTACTTTAAGTTGAGTTAATATCTCAATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGAAAAGAAACTCAAACTTTTACTTATATTTCTAATTATTTTTTAGCTATAAGTATGATCCTTTTTGGATACTTTTATCACAATAAAAGAATTCTAAAGACTTTCTTTTTAAGTGTAGTTTACATTAGTATTACTTTTGCTATTTATTGAACTTTAATTTTTCCAAAAATATTTGGATCAGAGTATGGTTCCTTTAGATTAGCTTTAAGCACAATTGTACATGCAATTAATCCATTAATTGGAATAGTCATATTGATTTTAAATCGTAAAAATATTACACTTGATAAAAAAGTTATGTACATGTCTAATTTAGGTGTTATTGGTTATTTATTATTTGCCTTTGGCACTTTTTGAATTGGACTAAGTTATTCACAAAACTACAAAAATGATGTAGCAAATAAACTAAATCAATATGTGGATAGTGATATTGTAGTTTATGGCTTTTTAAACTTTATTGAACCATTTTTTTATCGCGGAGGTAATATTGCAATAGTTATTACATTAGACTTATTAATGATTATAATAGCAGTCTTTGTACCTATTGGCTTAAGTTATTTTTGAAAAGCAGTATTAAGAATAAAAATTGAAAAAAATAATGAAAAAGATAGTTTATTAGAAACGAAAAGTGATGAAAAAATAGCAGAAGATATTAATTAAAAAAATATTAGGTTTTTAAAACTTTTAATATAAAATTAAAGAGCATATAACAGCAAGTATTCATAAAAGAAAGATAATAAAGTTTCAGATTCGATTTGAAAGTCGGTTTGCTGATTAAACCAAAATCATTAGAACTTTATTAATTCATTTAGAGAATACTCTCTTCTTGTTTTATGCAGAAAGAGGAAACTTATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGATTAACAATCAAAGGTTTTGAAGTGTCATATGACCGTTTACCAGACAGAAGTGAATTACATCCAGAAATTAAAGAAAACTTTATCGTTGAGTTCTACTCAAAATAATAATTAAAGGAGTAAGCTTATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAATTTTACTAATAAGAGTTTTTAATACTCTTATTTTTTATTTTTGGCACTTAAAAGATTTATTGACATATAAATTTATTTAAAAAATTACCTAAAGCACTATAAAATAGGTCTTTAAAACACAAAGTTAATTTAGCACTCTTTTTAATAATTACTATTAATATTATTAAATTAAGATATAATTAAATTATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGACAAACAATTGAAAGTGAAATTGATGAAGACGAAAATATAAAAATAGCAATTTGTGATGACCACAGTACCTTTATTAGTAAGAAACTAGAAACACAAAGCAAAATTAAAGAAATAGCACTTGTCGGCACTAGCCGAATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACGGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAATTCAAAATAGGTCAGTGATTAAAGTCACTACTTATTTTTTATTAAAACAACTATAAAATAGTTATTTAAGTAAATACAACTTTAATTTTTTTAATTATTTTTCAATTTTTTTATGAAAATAGTATTCAAATATATTAAGCACTAATAAAAATAATATATAATTAGATTATGTTTAAAAAATCATGTAAAGATGCTTGAATAGTTGTCAAACGTGGCAATATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAAAACAAGAAATTGCAAAGTGTAGAGTGAACCCCAAATTCCGAACTTTTTGGAAAGGGGTTCATTTTTATGTAATTTTAATTTAAAAAAAGAAACAAATGAAATAGATATATAAATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAATAAAAGCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATTCAAAGGTGATGCTCATAGAAATATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGGAACAGATGTTACCGAATTTAAGTTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAACGCAATTTAAAGCATGCAAGAAAGGGAAATTGTTTAGACAATAGTCCTACCAAGTGTTTATTTGGTGTTATAAAAATAGAATTTTGATTTGGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACCACTTTAGAAGAATATATTTCATATTATAACAATGATAGAATTGTTAATGAATTAAAAGGACTTAGCCTTGTTCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATGTTTGAGGTTCATACTAGTGTAGCTTTGTAATTTTTTTACCTTTTTTCACAAATTTTTCCATTTTCGCCTTTATTTATTACCAAAAAGAAAAGTAAAAAATGAAAGGAGGACTATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGAGATAATTCTCCAGCAACAAATGGAAATATCTTTTATGCGTGATAAAAAAGAGCAGGGCAAAAGCCTTGTTTTTGCTTTTTATTTAGCTAAAATTATTCAAAAAATATTTTGTTCATTATTTTAATAATGTGGTAAAATGAATAGGCAATATTTAATTGCAGAAAGAAGAATCTAATTCTCACCTGATGGCCAATAGCCTTGGGTTTATGCTACAATTAAAACCTAATTTTAAGTTTTATGTAGTAGAGAGTGGGAAACCACTTTTACTTTTATTTTTAAAAATTTATCTATAAGGAGTTTATTATTCAACCACAAGGAAAAATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAAAATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAATGCTTAACTTGAAGTTATTTAATTTATAATGAAAGGAAATTATCATGAGAGTTAAAGGCGGAACAGTTACAAGAGCAAGACGTAAAAAATGACTAAAATTAGCAAAAGGATATTTTGGCCACAAATCAATCGGTTACAAAGTTGCAAAACAAGCAGTTGTTAAATCATGAACATATGCTTTTAGAGACCGTAAACAAATTAAACGTGAATTCAGAAAATTATGAATCGCACGTATCAATGCAGCAACTAGAGCTGAAGGTTTATCATATTCAAAATTTATTAATGGATTAAAAAGAGCTAATGTTACAATTAACCGCAAAATGCTTTCAGAATTAGCTATTAGCGAACCTAAAACATTTGCAATGTTAATTAAAATAGCTCGTGATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCCCAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAACAATTGAACTAAAACAAGAATTTAAAAAGCTATTTTCTATTAAATCAATACTAGAAAAAATCAAATTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATGGTGAAGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGAAATTGATCTAAAATTTCTACAATGCTTGATTATTCTCTTGAAATAAGAACGCTTATTTATACCACTAATCCAATTGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAAGGGTTTTCACATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTGATTTTGAAAATGAAGAATAATTTTTTTAAATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTTAGGCATTATCTTATTTTATTCTGCAATATTATTAGGTGTTGATCTAAAGTTAATTCCTTTCTATTGAGAAAGGGAGAACTTTACACAAAATTCTAAACAATCTTTATTTTTTTAATTCAAATATTAAATTTCATTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTGCAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGACGAATTATTTTTCAAAATGTATTTCTCAGTGATTGCAAATTCTGATTTTCAGTACATTCTATTTCATATGTAGTATGAAGCATTAAAGTTTCCTAATGGGTTATCAAGAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGTTCACCATTATGTTTTAATAAGAAAGTAGTTGAAGCATGATCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGCACTATCATATTTTTCATTTGAATTGTAAGCAGATGATCTATAGTAAGGACCATTGAAATCACTTTCAGTTGGATTTCCATCTGGACCTGGAAGAGTGGGGAATGGTTCTTCTTCGCCATGAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTTTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTATTGTATTTAATGAAATCGAAGGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCTTTGAAAATCATTTTTAAACATTATTTTAGAATCTGATGTTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTCTTCTCATATTCTATTAATTTGGCTAAAGTATATAGATCATCATGTCCACTTCAAATATCATTTTGTATTGCTTCTAAATAATCACGTGAATTCATATTAGGATATTTTTGATTTATATATTTAACTGCTGGTGTGTTACCAAAAAGCCTACGGTTAATAATTGTATGTCAAGGCACATAATATTCATTTTCAATTTGTTCACCTTGTTCAATTTCACTAGTACTATTTGTTATATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTTCAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTGCCTAGAGCTTTAGGTGTAGTTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTCTGAGACAGACGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTGAACGGCTAATAAATAATCATTTTCTAAATAATTTTTAATTGAATTAATTTTTGCAAAATCAACGCCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACAGCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAATAACACTAAAGTTTTCTGTAGAATTAACATTTTTATAAATTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCTACATTTGCTATTGCTGCTAATTTTGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAATAAAACCATTGATTAAATTAATTTTTTCAAATAGTTCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTGTTTTCAATTGAAGATATTTCTTTAGTTGATAAATCTGGTTTGTTATTATTAATATCTTCAATTGTTTTTTTAGCATCTTCGAATTGGTTTAATGTTTTTAAATTATCAGAAATTTTTGATAAGAAATCAGCAATTTTTTTATCTGCATTGCTAATAAGTTTTGAAATTTCCTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCATTTTTAATAATTTCGAATATATCTAAATAATTTGTTTCATATTCTTTAAGATTTGAAATTACTTCATAGAATGTTGAAATATTATTAATTTTGTTATCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTTGTTTGTATATCAATTATTGATTTATAAGAAGTTTCGAATAAATTAAATTCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAAGGCTTTTTTAAATCCATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATAATCATTAATTAATTTGTTTCTACTTTCAATATACTCATCTCATTTAAAAGAACTCAATTCAATTTTAGATTTTTGTACAAATTGATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTCAATTGAATCAACATTTGAAATATAAGCATTTAAAAATTCTTTAATAACAGACTGAATAGAAGTTGTCAAATCATTTTCAGCGTTTAGAATAAATTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAATAAATGAGTTAAATTCATTAAGAGCATTAACAATAGGCGTTAATGATTTGCTGCTTAAGTCTAAAATTTGAGATTTGTTAAATAATTTTGTACTTTCAATTGTTTTGACAAAAGTGTTAACATAACGATTTATATATTCCTTTGTATTTCCATATGATTGTTCTAATGTTTTACATGCCTTAATAATATCTTTTATAATAACACTTCTATTTACTAAAACACCACCATTTTCTAAAATAACGTCATAATTATTAGAGTCAAATCTTGATAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTATCTATGTTTGACATGAATTTTTTAATTAAGCCGTCTTTTTTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCCTTATTAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTACTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAGTGTTTTGATAATCTTTAATTTTAAGTTCTAAAGAATTTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCAGTTTTTAAAGTTAATAAATCATTACTACGAACAACAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTACCATTTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTATGTTTAACAGTTCTCATATAACTTTTGTTACCAAAGTGGTGACCAAGATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAGTCAATTTGAGTTTGACACAAACTTAGTTCATTTCAAAGACTCGAAAGTCATGCTTCATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAGGTATTTAATATTGTTTTCAATTCACAACTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCACCAACAATCCCGGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAATATAAAAAATTAAATTTTTGGGACATAAGGAAAATTATGTCGATAATTATGTCCTTAAAATCCTTTTTTATAGTTGCATAAGTAAAATGCAAACATATATTTAAATTAATTTCAATATAATTATAGCATTATTTTTATTAATAATAAATATTAATATTTTTATTTTATCATTATTAAATAGCAATTTTTATTAATTTGATTTTACCAAAGTGGCTATTTTATAATGGATTTAGTAAAATGCAGAATTTTTTAAAATTTTATTTAAATATGATAAACATTATTTTTTTAGTTTTTTATTTTATTTTTTAGCATCTCAATTTCTTCTTTTAAGCTGCAATTTTCTTTCTCTAATTTGATAATTGTAAGCTCTAAATTATTATTTATATTTAATAATTGCATAGAATTTAAGCCTATTTTTTGATCTTGTATGGCTAAAATAGCATACTCATCCATGATACTTTTATCATAATCAATATAAGTTTTTTGAGTAATATTTGAATTTGTGTGTCCTAATAATTTGGCTACTAAATCATTATTTTTTAAATTATAAAAAGCTTTAGTTGCATAATTATGTCTTAAGACGTGAGCCGAGATTTTATATTTAAAATCAGGATATTTTTCACTAATATGTTTTTTAAAAAGATAAAACATATTTTTAATTGATTTTTCAGAAATACGTAATTTATCTATATTTTCTAATATATCTTCTCTAAGATTACCTATTAAAGGCATCTCTAATTTTGATGGAATCCTAAAGTATCTAGGATTGTTGTTTTTTAATGTTTTAATAATAAAACTTCAATTCTCTTTTTCTAATTTATCAATGTTTTTAATAAATTCTTTATATTCTCCAACTCTAATACCAGTTGAAAGAAAAATACGAAAAATGATACTAAATTTGGTGTTTTTGTAGCTTTTTAATTCTTCAACAAGTTTAATTAATTCCTTTTCAGGTATAGCTTGTCTTTTGCCTTTTTCTTCTTTTATATATAGAAGTTCATTTTTAACATCCATATTTGTATTAGTGATTTTGCAAAATCAATTTAAAAAAGAAGAAAATATGTTTCTTTTAGTTAATTTAGTATTATTTGAAATTTTATTATTTGCTAATTCACGATTCATCCTTACAATTAATTGCTTCGCATTGTTTCAATTTTTTAAGGATGATAAATACCTTTTATATTGTTTAAAAGTTTGATAACTTCTTTGGTTTTTTTGAACCATTGAGTAAATATTATTCAAAAAAATATCAAGAAAATTATTATTCTTTTTCATGTTTTTTGACCTCCAAAAATGTCTAATAAATATTTTAATATAAAGACATAATTTTCCTTATGTCCCGAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAGCATAAAATACTTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAATAAAGATGGTTTAATTAAAAAATTCATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTAAAATTCTAGACTTAAGCAGCAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAGTTCAAATGTCGATTCAATTGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGACCAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGAGATAAGTATATTGAAGGCAGAAACAAATTAATTAATGATTATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATGGATTTAAAAAAGCCTTTGTTAATATTAAATCAAGATTAAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAGCATTCTTTGAAATAATTTCAAATCTTAAAAAATATGAAACAAATTATTTAGAAATATTTAAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAAAATTGTTGATTTCTTATCAAAAGTTTTTGCTAATTTAAAAACATTAAGCCAATTTGAAGATGTTAAAAAAACAATTGAAGATATTGATAATAACAAACCTGATTTATCAACTGAAGAAATATCTTCAATTGAAAACAGTGAAATTCGAAATGACAAGGAAAAATTTAAAGGACTATTTGAAAAAATAAATTTAATCAATAATTTTATTTCATCGATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCGAAATTAGCAACAATGGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCAAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATCTGTCTCACAAAATAATGCAAATTGAGATGAAAAATCAAAAATTTGAGCTAAACCTAAAGTTTTAGGCAAAAAACATCTATATGATCGTATTTCAGATTTAGATACTGGAGGATACTCAAAAGGTAATACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCTGAATATTCATGGCACCGATATGGAGTAGATAAAGGAAATAGTACTAGTGAAATTGAACAAGGTAAACAAATTGAAAATGAACCTTATAAGCCTTGACATACAATTTTTGACCCTGGTGATCATGGTAAACCAACAGTTAAATATATAAAACAAACATATCCTGATAAAGATTGAGCAGATTATATAAAAGCAGTAAATGATGATATTTGAAGTGGACACGATGATCTATATACTTTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGAGAAAGATGAAGTGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTATAGTTTATGATGAAGAAAAAGGGAATTGATACGGTCCTCTTCCGGGTCCAGATGGAAATCTAGTAAAAAGTGATTACAATGGTCCTTACTATAGAACAGAAAATGGAAAATCAGATGAAAAATATGAAAGTACTAACCCACTATTTGAATATATGCCATTTTCTAATCCATTATTTTCATATTTAAGAACAGTAGACCATAATTCAGTTCATTTCTTATTAAAACATAATGGAGCTCCTATTTCATGAGTAAATTACATCTTTAAATTTACTCTTGATAACCCGCTAGGAAACTTTAATGCTTCATACTACATATGAAATAGAATGTACTGAAAATCAGAATATGCAATCACCGAAAGATACATTTTGAAAAATAAATCTTCTGAAACAGCTTCAATTTGAGATAAATGATCAAATATCTTTACTACAACAAGTAATAATACATATAGCAAACTTATTTGATAAATTAATGAAATTTAATATTATAATGAACCCTAAATTCCTTTTTCAAAAAGGGGTTCATTTAATATTTGGATTAAAAAAATATTATGACTAATCCATACAAAAAGTAATTATTATTTTTAAGTTGGACTACAAAAAGTTTTGTGTAAAGTTTTAAAATCCTTTCTTAATAGATAAAATATTATCAAGAAAGGATTTTTTATTTATGAAAAGAGAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAATTTTAGAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCACATTCCAAGAGATCGTGAAGCAAAATTTGAACCAATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGATATTTGTTTTATATTCTAGAGGAATGCCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTCGAAGATGTAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGATAATAATGTTGTAACTAAGAAAGCAACATATGTTATTATGGGTATTAATGGTGATGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCTGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAAGATATGAAAGAATTTTGCAATGATTTAAAATCCGTATATCAATCTAATGATGTAAAAAATGAAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGAAATTGATCTGAAATTTCTACAATGTTTGATTATTCTCCTGAAATAAGAAAGCTTATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTTCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATAATTAATTTTGAAAATGAAGAATAATTTTTTCAAAATTTTAAAAGCTTTGCTTCTTTAGCTTCAAAGAAGGCCTCTCCCTTAATTGAATTAAGGGAGAGGGGAGAGGAATTAAGATTTCTTGCATAGCAAGATCTTTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTTAGACATTATCTTATTTGTTCTACAATATTGTTAGGTGTTGATCTAAAGTTAATTCCTTTCTATTGAGAAAGGGTGAGACTTTACACAAAATTCTAAACAATCTTTCTTTTTTTAATTCAAATATTAAATGAACCCCTTTTGAAAAAGAAATTTGGGGTTCATTTAATATTAAATTTCATTAATTTATCAAATAAGTTTGCTATATGTATTATTACTTGTTGTAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGACGAATTATTTTTCAAAATGTATCTTTCGGTGATTGCATATTCTGATTTTCAGTACATTCTATTTCATAAGTAATATGAAGCATCAAAGTTTCCTAGTGGGTTATCAAAAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGTTCACCATTATGTTTTAATAAGAAAGAATTTGAAGCATGGTCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATGTATGAGCAGCTGCTCTATAGTAAGGACCATTAAAATCACTCTCTGTTGGATTTCCATCTGGACCCGGAAGAGTATATGATTCTCCATAGTTGTTATGAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTGTTATATTTAATGAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCGTTTAAATTAACAAAATTGTCTTTAAAATCATTTTTAAACATTATTTTAGAATCTGATATTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTTTTCTCATATTCTATTAATTTGGCCAAAGTATATAGATCATCATGCCCACTTCAAATATCATCACCTACTGCTTGTAAAAAACCATTTGATTTCATATTAGGATATTTTTGACTTATATATTTAACTGCTGGTTGGTTACCAGTAAGACTACGGTTAATAATTGTATGTCAAGGTTTATAATTATCATTTTCAATTTCTTCACCTTGTTTGATTTCACTAGTACTATTTCCTTTATCTACTCCATATCGGTGCCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAAACTTTAGGTTTAGCTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTAAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTTAACAGCTGATAAATAATCATTTTCTAAATAATTTTTAATTAAATCATTATTTGTAAAATCAACACCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACACCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAACAGCACTAAAGTTTTCTGTAGAATTAACATTTTTATAAGTTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCTACATTTGCCATTGTTGCTAATTTCGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATCGATGAAATAAAATTATTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAATTTGTTTCATATTTTTTAAGATTTGAAATTATTTCAAAGAATGCTGAAATATTATTAATTTTGTTGTCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTAGTTTGTATATCAATTATTGATTTATAAGAAGTTTCAAATAAATTAAATCCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAATGATTTTTTAAATCAGTTTTTTATTGAATCTAATTCATTTTTGCTATTAACATAATCATTAATTAATTTGTTTCTGCCTTCAATATACTTATCTCATTTGAGAGAATTTAATTCAATTTTAGATTTTTGTACAAATTGGTCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTCAATTAAATCGACATTTGAACTATAAGCATTTAAAAATTCTTTAATAACAGATTGAATAGAAGTTGTCAAATCATTTTCAACATTTAGAATAGCTTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAACAAATGAGTTAAATTCATTAAGACCATTAATAATAGGTGTTAATGATTTGCTGCTTAAGTCTAGAATTTTAGATTTGTTAAATAATTTTGTATTTTCAATTGTTTTGACAAAAGTATTAACATAACGATTTATATATTCTTTTGTATTTCCATATGATTGTTCCAATGTTTTACATGCCTTAATAATATCTTCTATAATAACACTTCTATTTACTGAAACACCGTTTTTTTCTAATATAACGTCATAATTATTAGAGTCAAATCTTGACAATAAAATCTTTTTAACAGCATTGATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTAGATATGTTTGACATGAATTTTTTAATTAAACCATCTTTATTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCCTTATCAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTGTTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAATGTTTTGATAATCTTTAATTTTGAGTTCTAAAGAATCTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCGGTTTTTAAAGTTAATAAATCATTACTACGAACAATAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTATCATCTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTGTGTTTAGCAGCTCTTATATAACTTTTGTTACCAAAGTGGCGGCCAACATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAATCAATTTGAGTTTGGCACAAACTTAGTTCATTTCAAAGACTAGAAAGTCATTCTTGATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAAGTATTTTATGCTGTTTTCAATTCACAATTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCGCCAACAACCCCAGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAACATAAAAAAATTAAATTTTCGGGACATGAGGAAAATTATGTCATTATATTAAAATATTTATTGGACACTTTAAAGGTTAAAAAACATGAAAAAGAATAATAATTTTCTCGATATTTTTTTGAATAATATCTACTCAATGGTTCAAAAAATCAAAGAAGTTATCAAACTTTTAAACAATATAAAAGGTACTTATCATCCTTAAAAAAATGAAACAATGTGAATCAATTAATTATAAGGATGAATCGTGAATTAGCAAATAATAAAATTTCAAATAATACTAAATTAACTAAAAGAAACATATTTTCTTCTTTTTTAAATTGATTTTGCAAAATCACTAATACAAATACAGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGACAAAAGACAAGCTATACTTGAAAAGGAATTCATTAAGCTTGTTGAAGAATTAAAAAGCCACAAAAACACTAAATTTAGTATCATTTTTCGTGTGTTTATTTCAACTGGTATTAGAGTTGGATAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAAAATTGAAGTTTTATTATTAAAACATTGAAAAACAACAATCCTATATACTTTTGGATTTCATCAAAATTAGAGATGCATTTAATAGTTGTTAATTTCAAAAGTGAAATCTCTAAAACCTAGGGGTCTCACTTTTGAAATTAACAAATTTTTAAAATAAGATAATGTTTTTAGGCATTATCTTATTTTGTTCAACAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTTTATTGAGAAATGGAGAAACTTTACACAAAATTCTAAACAGTCTCAAATTAATAGATAATATTATTAATTAGCATTTAAATTTTGAGTTTCCGTTTGTTCTTTAGTTTCAATTTCTTGAGTTAAAATTGATTCTCATTCCTTTGTTGATTCTTCAAAATCTTTAACTGGAACTTCTTCTCTTTCAAGATAATCACTTTGTTTAATTTTATCGCCTTCTTCGATTAATTGTTGAAGTGTTTTTTCTTGTTCTTCAATTGCATTTTCAGCTTCAATATTTTTTGTTTCTCCCTGTTTTATAGTGTTTTCAGTCGAATGTGAAACACTTTGAATTTGTTCATTTTGTTCTTCAACATTAAGCTCAATAACCATAGTTGCTTCATCTTTAATAAAACCAACTTCTTGAGTTTTTTCTGCATTCAAAGTAATTGTGTCTTGAGCTGTTTCTTCTTTTTTGATAGCTTCAACAACTTTTGTATTTTCTTTAGATTTTTCAATTTCTTGAGTTTTGGTGTTTTCACGATCTTGGCTAATTTTTTCTAAAACTTCTTCAATAGTTTCATCTTTCTTTTCATTATCTAAAACTGGCACTTCTTTAATTTCTTCAACTATAATAGTATCTGAGTGTTCTTGACTGATAAAGTCTTCTTCATTAACGTTAATTACTTGACTATTTTCATTAATTATTATTGCTTTAACTTGATTTTCTTCTTCAATAACTTCATTTGCTTCTTTAATTTCTTGGTTTGTTGCTTCAAGAATTTCTAATTGAGTTGTTGGTATTTCTTTAGTTACTTCAACTACTTGAGTCATATCAACTGGTTCAATACTATTAATAGTTTCAAGAATTTCTTCTTTTTTATCTTCTCATAAAATTCGATCTAGTTTATAAACAATATATTTTTTAGATCTAATAAATTTACACAAGTCTTTAAAAATAACAAAGTCTTCAGGATTATTAACATATTTGTAATCATCGATCATAAATGTCATTATTGGTTCATGTTTTCTTCTTAAAACATAATCAACTTTAAGTGTTCCAAGTGTTTCATTTGGAATTAATTTTAGATAAGGTTTATCTTTAATTGCTTCAAGAATTGTGCTTTTGATTTCTTGATTTAATAATGAATTCTCAACTTCAACTGGTTCAGTTTCAATGGTTTGCAATTGATTATTATTTTGTTCTTCATCTTCTAAGTGTAAGAAGTTTTTTCTTTCAATGTCACTTAATTCTAAGAATTCAAGTCAATGTTTGAACATAATAGCATCTTCATTACCTGTCATTATAGTTAAGTCACTAGATTTAAGTGATTTAATAACAATCATTTTATCCTTAGCACGACTAACTGCCACATTTAACGCATTCATACCGCCAGGACGTCCAACATAAGTTGAGTGAATTGCTGTATCTTTATCATAAGCTACTGAAGCTATAACTAAATCAGCTTCATCGCCTTGAATATTTTCAATGTTTCTTAATAACAAGTTTCCATTATTAATTGCTTTTTCTAATTCAGGATGATTTTTGAAAATGCTTGTGGTAATTGAATCTTGTTGTTTAGCATTGAAGCACAACAAAATGATTTTGTTGTATTTATTAATATTTTCTTTAATAAGTTTGATTGCTAAATCAGATTCAACTGTGTTTTGATTATTTTCTCAAAGGCCATTAACTTCATGTACTTCAATTGGCTTGAAATTAGCTTCCATATTAGCTGAATCGATAACATCCAAAGTACTTTTGTAGAAGTATTTAGAGCTAAAAGTCATCAGTGAAGCATAATTTGAACGATAGTTTTTGTCTAAAAGCACACTATAAACACCAAGACTTTTGGCAAAGTCTAGAAGGCTATCTACTTTTCCATAAATGCTTTCGTCATCACTAACTCTGACACCAAATCAGTTACTTGGTTTCATTTGTTGATCATCACCAGCAAGAATTTTAATTTTTGCTAAATATAATACTGGTAAACCTTTTTCAAGGAAAATTTGGCTTGATTCATCTAGGATTGCATAGTCAAATTCGCCTTTATTTCAGGCACTTAAGTCAGCTTCGGGTGTCATAATTACAATAGGGAAAATCTTTTTAATCATATTAGGAAAACGCTTAATAAACTTGTAAGGTTCTAATGTTCCTAATCTAATTGTGGCTGCAAATTCTGAGTATTGATCTTTTTCTTCATCAGTAAATTTGTTAATTCGTTTAATAATACCTTGAGCAATAAATTTCTTTAATTCATTTTCATCGCTAGTTTCTTGTGTATTATTAGAAGGCAAAATGTTTATTAAATCATTTAAAAATTCAAAGTCTTCTTGCTTTAAGTTTTTTAAAACTTGAACTGAATCATTTAAATTTCCTTGAAATTCTTTAAATGTGTTAAACATTTTTTGGGCTGTTAATTTAATTAATCAGTTTTTGAAATGTCAGAATTTGAATTTCATTTTGTTAATTCTAAGCATGTCATTAGTTAAAGCTTTTGAATCATCAAAGTGAAATTCGGCTGGATATAAAATATTTTTAGGCAATTTTAAAATTGCATTTCAGATTTCTTGTGAAAATGATGATCAATTATTATGAACTTTAGCTAAAGCTTGAGTGATATTAACAAAACGTGGATCACTACTGAAATTAGCTATATTGTTAACAAAACGAATATCTTTTTGGTTAATGATTCTTAATGGTTTTAAATTAGGATTATCATCATAGTTTTCTAAATAATCTAAATATAATCTAAGAGGCAAGTAGAAATTCTTTTTGTTCATGTTTTTTGAATTAAGCATGAAAAGACAAAAGATTTTTAAAGCGCCCATTCTATTTTTAATAACTTCAAGAGCCGCTTTCTTTTGAGAACAAACTAAAGAATTTTTGCCATAAACTAAAAGGTTAGTCAAAATATTAACAATAGTTTGGCTTTTTCCTGTACCTGGAGGACCTCAAATAATTGTGTTTTGATTTAAGCTTGAAGCTATAGCTTTATCTTGAGAATAGTTGGTAGGTGTAATTTTAAAAATAGATGTTTTAGGGTTGTAAATTGTTTGTTTTACCCTATTTTTATAGATGTTTTTGTTAAATTCTATATCTAAAATTTTGTTCAATTCTTTATTCTTAATTATTTCAAGCATTCTATTTCTGGCATAGCCACCACTTGGTTGGAATAAGCCTAAAACTACGCCTGGTTCAAATTCCATCGCTTGGTTGCCAATAGCTTCGGGAATTCAACGTTTGAAAGGTTGATTTAAGTCTAAGTTAAAGTTGTAAATATCTTTTCATTCTTCATTTAAATATTTAATTAATTCTTTAATTGATCAATCACCATAGTTTTCCGAGATATTTAAATCAAAACCTGCATTACTTAATAAGAATAGCAATTTTTCATTAATTTTAATGTCACCATTTGAAACTAAATATGGTCTAGCATTTCTAATTTCTAAATAAACTTCTTTTAAAAATAAAGGACCATAAATAGTTTTATCGTCAATAACTAATTTAACAAATAAGAAACCTAAGTGCATAGGTCAAATATTAGTTTCAGTGTTAATTTCATTAGCTTTATTTAAGAATAACTTTCATTTTAATGATTGTTTTTGAAAATTAACTTTTAATTCATTAATAATGTTATTTTTAATTCCAAGAGGATTTTTCTTTAATTGACTATTACGTTTTTCACTTACTTCAATATTGTTTTCTTCAAGAATTTTAATAATTTCTTCATATGTATTTGCTTCTTCAAGTAAATTACAAATTCTGATCAAATTAACTTCAAGAATAGGTATGTCAAAGTTTTTATTGTTGTAAATTTTGCTAAAACTTTCAGGTCCAAAAATAGTATAAAAATCAAAAGCAAATTTGTTATTTACTCTAGTAAATACTGCACTGTCATTTGGACTAACATCTAGTAAATTGTCTAGAATACTTTGATATTGTTTTTCTTCATTGTTTGACATGATATTGACCACCTCATCTATTTATATATGTACATTAATTATAAATATAAAATTTAATATTAATAATAAATCATTGTTTGAAAAAGTGCATTATTTATAATTATTTTTTTCAAAAATCAAGTGCAAAATAAAAAAGTGGCATAACCACTCTTATTTTTATTAATATCAAAATTATTGTTGTTCAGCATTATAAGAATTTGTTGTTTCTCAAACATTTTTCTTTTCTTTACCTTTAACGCTTTCAATAATAATACCAACAATAGAAACTACCACAACACCAATGTGTGTAAAGAATTGGATAAATGGTATTACTCCACCGATGAATTTTTCATCGCTTTCTTTTGTCATTTTGAATGTTCCACCAAAGAATTGGAATACTAATCAAACTAACATTGTTACAAATACAGCAAGCATAATTGGAGCTATAAATCAGTTTTTGTTTACGAATGCTTTTATTGTAACTGCTAACACTGTAAAGATTCATGAAATATAAATGATAAAATACAATGCTGAGATTCCACAAGCTATATAAACCATTGTGTCTGATTCAACTACGCTTTTAATTATTGAAAAATTACTTGTGAAGTTTGAAAAGTCTGCAAAAATTCCATTTGTTACAAGTCTGTTTGCTATTAAAAACTTACCTGCATCTCCACTTTTTTCACTTACTTTTGCCATTCAACTTCCTTTAGCAACAATAATTAATAAAACAGTAAATATTGCTAAAATAATTGACAAAGTAATGACAGAAGCGTATTTAATTTTTTTGCCTTTATCTGACATAAACACCTCATTTTTTAATCAAATTTTATAGTTTTTTAATATATAAAACTATGTAATTATTATACAACTTTTTTTGTTTTTTGCTATGTAAATTATTTAATATTCTTTTAGGTTGTAAAATGATCTAATTTCACTAAAACAATTGTTTTATAGTGCTTTAGCTTGCAAGCAGGTCCTTAAGTTGTTTATATAATTCATAAATTCTTTGCTCATATTTAGTAGTTTCAAAGGGCAATATTTTTTGAGCATTTGCTCATAAAACAACGATATAATTAACCAATATTTTGTGTCTTAAAAGATAAACTAAATTATATTCGCCATAGGTATCTAAAAATAATTTTTCTTGTTTTTTGTCTAACTCATTTGCTTCAATATAATAAGCAAGTTCAAAATGTTTATCACCTAGCGAAGCATATTCTCAGTCAATAAAATATACTTTTTTATTTTTAACTATCATGTTAAAAAATCAGAGATCATTATGCAGAGGAGTATCTTTTCTCATTTTTGAAAGTGTCATATTAATAAATTTATAAAAATCATTCAACACTTTAATTTTAAGTTTCTTTTCTTGAATAATTTTGCGATATTCTTTAATTCTAGCTGCATGATTAGAAGCAGGAAAATTAAGTTTTGAATTGTGTAAAATTTTTAAATTTTGAGCTACTTCCTTTATATTATTATCATCATTTCAGTATTCTTTACCATCAATATATTCTCAAGTGATTTCTTTGTCATTTTGGCTAATAAGTTTTGGTGTAAAACTAAAATCTTTTAATAAACTGTATTCTAATTTGTGATTAAATTTATTTAAAATTTTTTCTTGAATAAACTTATTTTCATCTCGATATGAAATGTTAGTATGTCCTTTTTTAATTAAAACTTTCATTTTTACTCCTTTTTTTATTTCAAAAATTATATATTATATTATTATTTATTTAATATAAAATTTAGCTTACTTAAATAGGTGAAGATAATGGACAAAAGCAAAATTAGAAATTTTTCAATTATTGCTCACATAGATCATGGTAAAAGTACGCTTGCTGATAGAATTCTAGAAATTACTAATACAGTTAGTGACCATGATATGGATGATCAATTTTTGGACCAAATGGATTTAGAACGTGAAAGAGGAATCACAATTAAATTAAATGCTGTACAAATTAAGTATAAAGATTACGTATTCCATTTAATTGATACACCAGGTCACGTTGATTTTACTTATGAAGTTTCACGTTCATTAGCTGCTAGTGAAGGAGCATTATTATTAGTTGATGCTACTCAAGGAATTGAAGCTCAAACTTTAGCTAATGTTTATTTAGCTCTTGAAAATAATTTAACTATTATTCCTATTATTAATAAAATTGATTTACCTTCAGCTGATATTGAAGCCACAAAAAATGAAATAGAAGAAGTGATTGGTCTACCAACTGATAATGCAGTTTGCATTTCAGCTAAAACTGGTTTAAATTGTGAAGCAGTTTTAGCTGCAATTGAAAAATATATTCCAGCACCTAAAGATGCTGATGATTCTAAACCTCTTAAAGCTTTAATTTTTGACTCGTACTTCGATGAATATCGTGGTGTAGTTATGCTTATAAGAATTTTTGAAGGTGAATTAAAAGTTGGCGATGAATTTATGTTTATGTCGAATGGACAAAGATATTTTGTTAGTGAACTTGGAGTTAGAAGTCCAAAAGAAACTAAGAAAAAGAGACTTGAAGCAGGTGAAGTTGGATATGTAGCAGCTACTATTAGAGATGCTAGAGAAGTAAGCGTTGGAGATACTATAACTTTAGTTAATAGACCAGCTGAAAAAGCACTGCCAGGTTACAAAAAGAAAAAACCTGTGCTTTATACTGGGTTTTATCCAATTGATACTCGCGACTATATGGAACTTAAAGAAAGTTTAGAAAAAATAGCTCTTTCAGATAGCTCAATAACTTGAGAACAAGAAACTTCTAAAGCTTTAGGATTTGGTTTTAGAGTTGGATTCCTTGGAATGTTACATATGGAGATATTGCAAGAGAGATTAAATCGTGAATATAAAATCGGTATTATAGCAACTAGTCCTTCAGTTGAATATGATGTTCATATGACAGATGGCAGCATTAAAAAAATATCAAACCCTGCACTTTTACCTGATCGAACTTTTATCGATCATATTGATGAACCTTACATTAAAGCTGAGATTTTGGTACCTAATGAATACATAGGAAATGTTATGGAACTATGTCAAAATAAACGTGGAATTTATATCAATATGGACTATATTGATGCTAATAGATCAAGGCTTATTTATGAAATGCCACTAGGCGAAATTATCTTTGATTTCTTTGATAGACTTAAGAGTCTAACTAAAGGTTATGCATCATTTGAATATGACCTAATAGGCTATAAAACAAGTGATTTGGTAAAAGTAGACATACTTTTAAATGGTGATAAAATTGATGCTTTTTCAATTATTACTCATAAAGATTCAGCTTATAGTAAAGCTCGTGATTTAACTCAAAAATTAAAAGAAGCTATACCAAGACAAAACTTTGAAGTACCTGTTCAAGCAACTATTGGAAGTAAAATTATTGCTAGAGAAACTATTAAAGCTTATCGTAAAGATGTTACTTCAAAACTTCATGCAGCTGATGTTTCAAGATATAAAAAACTTTTAGAAAAGCAAAAAGCGGGTAAGAAAAAAATGAAAATGCTTGGTACTGTTGAAGTTCCTCAAGAAGCATTTTTATCAATTCTTAAAACTAATATTGATAAGAAAAAATAGTAAGGGATTTATTCTCTTATTTTTTTAAAATAATTTATTCGCTTTTGTTAAATCTTCATTTATAATGAATACCACAATTAAACATTGGAAGAAAAATTTTATTATGAAAAAAATAAGTTTTTAAGGCATTTAAGTGCTTTTTTACCTTTAAGGTTATTGCAATGAGCACATCTTGTGAAGAATAAGAAAAGAAGCCTGAATCAGTTAAACCTATCAAAAAGTTAGCTAAAAAATTAGTTGAAGATTTAAATAAATTGAATAAAAAAATTAGATCCGGCTAAATATGAAGTTAAATTAGAAATAAATTCAATTATAAAAAATAATAGTGAAGTAACATTTAATTTTTACTTACAATTGAAAAGCAATGCTAAAGTTAAATCAAAAATTAAACAAGTAAAAATTAATAATTTCAAAATAGTTAAATTAACAAAATCTTTTGAAGAATTATTTTTTGGAGTTAATGATTCTTCAAAATTATCGGCCAATAACGTTATAACTTTAGATCAATATGTTGATAATTTAAACTTATTTCAGAGCAAAATTAATCTTAATTTAAATTTAATTAATAAAGAACTAAAAATTTAAAAATAAGAGTTTATTTAAATGACAATAAATATAAAAAGTTAAAAACCTAATGATTTTAGCTTTAAGTAAAGAATTAAATTTAAAAGATATAGAAAATTTATTTTCTAAAAAGAATTTTTACAAGCTTATTTCAAATATGTAAAACTTGTTTCTGTAGACTCGAGAGAAAATATTTTTAAAGATAAAAATTATTTTGCTTCAAGATTTAGACAAGGTTTTGAAGATACAAATGAAGCTTATACAAGTAGATATTTATATACAAGCAAATTGCCTAAATATGACTTATTCAAAAATAATGACAAAAAGGTTATTTTTAAAACTAAATATAGTTATGATGATAAAAAAGTAGTGCATTTTTAAGTTATTATTTATATTTTGATGATGGTATTTATGAAAAGGTAAGAACTGACGAAAACCACTTTGAAATAGATGGATTCATTCAAATTAATGCTACTAAAATGATATGTTTTCAATAGTTGCTAATGATAAATTTTTGGGCCTAGCAGAAAGTGCCAAAAAATTTAAATATGTATTACAAGATTTAAAAAATAAAGGCGCTACTAACTACGAATGTGTTGAACGAAATATTTTAAAAACCGTATTTAATAGTAAAAAAACACAAATATTTTTATATCAAGAAAATAATAATTTGAGAGCAGAAAATATTGTTAATTTCAATCTTAAATATTTTAGCAATAGTGTTAAAAATGAATTAAATGATTCTTTACTTTCTAATGATGATTTAATAAAATTAAGCTTGAAAAAGCTTATGTTGAAATTACCAAAATATCTTTAAAAGGAAGAAACTAACAAATATATTTTTAACATTCATTTCAATTTAACTATAACAATAAAAAAGGTTGAAACTATAATTTAGCATCCTTCGATTTAACTTTTGAAAATATAATTTAGCCTATAAGTTTTCAAAAGTTATAATTTATTAAACTAGTTAATATATTTTTTTATTTGTTTTATTGATTCAAAATATTAATTTTTATATATTTTAATATATAATTTTTTAGTAAAAAAATATAGAAAAGTATTGTTTTAAGGAGGAAATATGTCTACAAAAACTATTTCAAAAGCAACAAAAGGATTAGGATGAGCTATTGCAACAGCTACTATAACTGGCTCAATTCTATTAACTATTATTGCAATAAAAACACAAAATACTAAAAAAATGTCTGATGATGATTTTCTAAAAAATTTAGAAATTAAAGGACAAAATGTAAGTGAAATTTATGCTAGTGATGCAAAAATTACAAATGTTGAATACAAAAAAGATTCTAATTATGAAATTAATGTTTCTCTAGAAGGATCAAATGTTCTAAAATCAACTGCATACTACAAAATTGAAATTACAAATAAAAATACAAAAGTTAAAAAGTCAAAAATTGTTCCAATAAGTGGTTTCAAGAAGTTTGATGAAAAAAGTACTATTTTAAAAGAAAAAGAGCTATTTGAAGATAAATCTAAATTAAAAGAATATTTAGATAATATGCCTAAAAATGATTCTGAAAATCTTAAAAAATATATTTCTAAAAATAAACATTTTATTTACAACGAAATACCAAGTTCATTAAAAGCTTCATTTAAAGGTTATTCTTTCATTCAATCTAATGGTCAAGATGCTGTTAGAGTTGTTTTCGTTTTAAATGGTCATATTACAAATGATAATCTCGAAAATAATATAGTTAAAAAAGCAAATAAAGAATATAAAAATAATGAAAAAACTTTTGAATTTTACTTAAGTAATTTAGTAAAACAAGGAACATCAAGTTCAGAAATTCAAGCAAGAAATAACTTAAGAGATTTAAAAGAAAAATACGAAACTCTTAAAAAAGAAGCTGAAGTAGCTAAATCGAAAGTTACAGGTGCTATTGTTGCTGAATATGAAAATGTTTTAGTTTCAATTACTAAAAATATTAATGAAATCGACAAAATTTTAAATGATGCAACAATTAAAGCAGCAGATCATGTTGATACATTAAAAGTTAATTATGAAAAAGACTTATTAGATAAAAATACTGTAGATTTAAAAGCAACAATTAATGATGCTGAAGTTACAAGAGATAACTATAAAGCTAAAGATGAAACAAATTATAACAATCCAATAATTGAATTTAACAAAGCTATTGATCAAGCAAAAATTAAACTTGCTTCAACAAATATTAAAGAAATTAATGATGAAATTTCTAACTTAAAAACTAAAAAAGAAGAGTTTATTAATGCTTTAAAAACTATCTTAACTGACACACAAAGTGATTCAAAAACTAAATTGTTAGAATCAAGCGATTACAAATATTTGATCGATGATATTAAAGCTCGTAAAGCTACTTTTGATGCTACACTTAATGAAGATAATATTCTAAGTGAAATTGAAAACTTAGAAGTTTTAGTTCAAAATACTCAAACAATTGATGCTATTACACCAAAAGCAATAATTGAAGCTAATAATAAACTTCTAGCTTCCTTACTAGCCTATAAAACAGCCTTAGAAGCAAAATTACACAAAATAAAACAAGATGTAATAGACTCAACTTATGACAAAGATTCATTGTCACAATTTATCGAATATTTAATTAAAAAAATTAAAACAACAAATGAAAGCAAAGCTTTAGTTAATAAACAAATAGATTTATTTAAAGCTTATTTAACTACACCAGGTTTTGTTCAAAAATATAATTCTTTAGAAAAAGATTTTGATAAATATGAAGCAATTTTTAATGAAAATATAAATAAAGCTAATGAACTTATCGCAACTCTAGATGCAGGATTAGATCAATATTCAAATTCAAAAGATAAATATAGTGAAATCAAAACTAAAGTTGACTCACTTTTAGCAGAACAAGCAACTCGTTTAAGCTCAATTGGAACTCAAATTGTTAATAGTGCACTTATTGATTTTGATAATTATGTTCAAAAGCAAAAAGCTTTAATCAATACTTCACGTAATAACTACAAAACAATTGTGACAAATGTTTACAATTTATTCAAAGATTCAGAAATCTACAAACAATATATAGATGATAATAAAGCGAAACTTACAAACTCAACTATTGATGTTAAAACTGCTTTTGAATTATTAAAAACTTTATATACAAAAGATAATGAAATGAATCAAAAAGTTGCAGCTCACTCAAACTTAGCAACTAATAGCTACTGAAATTCATTATTAGATGGCGATTTAAAAAATAACTTTACAATTCCAATGCAAGTTAATGCTGATTATGAAAATTACTTTAAATTAGATGACCATTACAAAATGGCTTTAGATATTATTATGGACAATTTAATATCTCAAATTCGTTCAGAACATATTAAAGGTATTGAAGCGAAACAAGAAGAAATTTATCGTATTTTCTATGAAAAAATGCGTGCTATTTATAATGCACAATTAACTAATGGAGTTAAATTTGTAAATGATAATATTCTAGATGCTAAAAATAGAAGAATTCAAGCATTAAAAGAATATGAAAAGGCTTTAAAAGAAGCAGAAAATTTAATCAAACATTATACAGATGAACACTATAACAATACTGATGATTTCTTCAAAACACTTGAAGAAGGCATTAAAGCTGTTAAAACTTTAAATAAAGATCGAGCAATGCTAAGTCTTGTAAAAAATACAACTATAGAATTAAAAGAAGTTATCAAAAATTCACAAGATCAATATAGAACATTCCTATTGTCTAAACTTGATGAAGCTAGAAAAAATTGACAAATTTATTCAAACAGCATCAATGGTCCAGAACATCAAGATGTCTTTCCAAAAACAATTAAACTAATTAATGAAAGTTTGGAAAAAATTAAAAATGCTATTGACAATAGTACAACTATTACTATTAAAGAATTAGAAGATTTAGTAAAAGAAAACAATGATGTATATGGAAAATATACAAGCACTAAAATTCCAGAATGAAACGCATTTATAAAGAAATTCGATAGTGCTTTTGAAGAATTAGAAGCAATCAAAAAAGACTATAAAAAAGAAACAATCGTCAAATTAGGTAAAGACAAATATCAAGACAATGACTTAGTTTTAAATCCTGCTTTTGAAGCTAATTCAGTTTATCAAAAAGCAAAAGAATACAAAGAATCTGGAAAATTAAGTGTAGAAAAAAATCTTAAAACATCTAAAGAACATATAACTAAAATTAAAGCTAAAATAGAAGAAATTAAACAATTAGTTAATTATTATGAAACAAAATGGAATCAAGCTAAAACTAATTTAGATAATACAATTAAAACAGTAGAAGAATATATAACTAACAATCCTTCAGCAACAAATATCGATCAACTTCAAGCACTTCTTAATTCAACAAAAGAAGCTATTGAAACTTCAAAATTAGAAGAAGTTAATTTAACAAAACTTCAACAATTAGATAAAGATCTTGAAGCAAAATATCAAGAAGTATTGAATAAAAACAATAGTTTAGCAGCTGCTAAAAATAAATTACAATCATCAATTGAAAAGGTTGAAAATCATTTAAATTCAAGAAATTCTTTAGACAATTTAGATAATTCATATTACTCAAATTTAATGCAAAATGAGAAAATTGATTACAATGAAAATAGTTCAACTGCTGCTTCCAAAAAAGATTTAAAATCTATATTAGATTCAATGAAAAATATTGTTAAAGGCAATGATGAAAATGCAATTAATCACAATGTTCAATTTGGTCAAAAATTAATTGATGCTATTGAAGAAAAAAGAACAGAAGACATAGTTGCAAACAAAATACATTAAAAGATAAATTAAATGAATATAACGATTTAAAAGATTCATTAAAATCATTATCTTCAAAAGATTATTATGGAGCTCAAATTACAAATCTTGAAGCTAATTCAACTGCTGATGAAGATGCTGATAGAATTACAAAATTAGCACGAGCTAACTTAATTAAAGAAAGCCAAAAATCAATTGTTAATACTAAAGTTGAATTAGAAAAATTAATTGGTAATTTAACTAAAATTAAAACTGATCGTGAAGCTCTCATTCGACAAGCAAATGATCAATATAATACAAATAAAGACAAATTTGCTGAGCTTCAAAAACAACTAAATGATTTAAATATTACACCTGCTACAATAGCGGATTATAACACAAAAGTGAAACCTAAACTTGAAGCAGCAAATAATAAAAATGAATTATTAAAAGAAGTTTCTAAAGATCAAAATAATAGTAATATAACAGCTAAAATTAATGATTCTAAAGTTGCAATTCAAGATTTAGAAAATGTTGTAAACAACTATAAAACAGCTCTTAACAACAAAATTAAAGAAAATATTTCAGCAATAAAAGAATTAGTACAATACATTAGAAACAATCCAAACAATTTAACTCTTCCAGCTAATTTTGTAACCAATTTAAAAGCTGTAGAAGACAATCAAGACTCAACTGATATTGCTATTTTAAATCAAACAAAAACTCAAAGTGAAGCATTAATTAAAGAGTTAACCGATTTAATTAATACAGCAGTAGCAACTGTTAATAATAAAGCTCAAGATACAAATCAAAAAGTTGAAGAAGAAAAAGCAAAAAATGAAACATGAATGACAGCTGCTCAAGTTCGAGAAATTCAACAACTTTTTGATAATTCTCAAGCCTTATTTAATGAAGTTAAAAACGCTGCTAATAATAAAGATCAAATCAAAGATGTAGGTGTTGATAACTTAACAAATAATATAACTAAAGCAGAAGCAAAATTAAAAGAAATTCAAAACAAAAAAGCAGAATTTAAACAAGAAAATACTAATTTAAGAAATCAAATTAGTGAATTAATGAATGATTTAAAAGTACAAGCTGATACACTTCCAAATGAATTTAATGAACACATTTTAGCAACTCATAGCTCAATAAAAACCATTCAAGAATATTATGAAAATACATATAGCAAAATTAAAAATCAAGAATGAGAAAACAAAAACATTAATGATTTACAAACTATTAAAGATATTCTAGATGGTTACAAACAATCATTAGATAATTACAAAAATGAAGCTGCAGCTGCTTATCGTAATAAATTAAAAGAACAAATTCAAAAATCAAATAATAAAGCTCAAGAATTGACTACCGCTAATGATGAATTAACTGGTATTAATAAAGTTGATAACTATTCAGATTCGATTACTAAATTAAATAATCAAAAGAAAGCAGAATATCAAGAATTAATTGATCAAAATAACAATGATCTTAAAACTCTTAAAAATGCTTTAATTGAAATTAAAAATAGTAATGACTTAATTGAAATATTAATTAATGAAGTTGTCAATAAAAAATGTAACTTAATTAATGCAACTATTAAAGAATATGAAGATCAACTAGAAGCAATTAATAATTCTATTTACAGCGATTTATATGAAGACATTACAACAAGTTTACATTTAGACTTTTTAAACTCTTTAGCTATTTTAAAAAATAAATATCCAAATAATTTAAATAAAGTAATCAAACTTTCAGAAGTAAAAAGAGATTATGAAGAAATTGAAGGTAAACTTAATGATTTAAAAAATAGATTAGCCGCTACACAAAATGAAAGAAATGACATTCTTGCAGAATTAAGATTAAACTTTGACAAAATTAATTCATATGCTAATTATTCAGATGCTGATAGTAAATTGCCTTTAACTAAAGCTTTAATAAATGATGCAAATAATAATTTAGAAAATCACTTTAGTTCAATGACACTAAAATACTTAAAAGCTGAAAAATCTGCTAGTGGTCAATATACAAAAGTAATAAATGAAATAAATCAATATGAAGCAGATCTTAATGTAGCTTTACAGGAAATTATTAATGAAGTAAAAGATGCTAAATATCAAAAAATAGATGACATTATTTCAAATAATTCAAGTGTCTTTTTAGCTGACAAAGATGATTATAAAAAAACAATTGCACCAGTTAAACAAAAAGCTAATAATGGTGTTAATGACTTAAAAACTTTAATTACAACAATTAAAGATGCAAATGAAAAACTTGTAGCTATTAAAGCTCTTGGCAATAGAATTCTTACAAAAATTAATACTGAATTCAACAATAGAATTAACCGACTTAAAACTATTGCAAATGAAACTGATGGATTCTTAAGTCCTAAAGTTGCTGACGGTAAATATGAAGGTTCTATTTTGCATGGAATTAAACAATCAAATGATGAAATTATTAAAATAATTAAAACAAAAATCGATACAATAATGAACAATAATAGTAACCTTGCATACTTAGTAAAAATCGATAATTCTAGTGATAAAACTATAGAAAAATTAGATCAATTAATTGCAAGAGCAACCCAATTTAAAGATAACGCAATCACAAAAACTAATGAATATGAAAGAGCATGAGAAGAAGCTAAAAATGAAAATCTTCAAAAACTAGATGAAGCTAATGGAATTATTCAATTCTTAAATGATAAAAAAACAACAAGCACAATAACTAGTGAAGAGCAAACGGCTTTAACTGCACTAAATACTGCTAAACAAAATTATGATCGAATTATTGAAAACAATATTGATCACAAATCAAAAACTAAAATTGAAGATGCTACTAATCATTTAAATGATGCATTAAATACTGCTCGTCAAGAATTGAAAAATTCACAAAAAACTACTGCAATTATTAATTTAAAAAACCTTAAATCAGAAATTAATGCAGACTTAGCAAAACTAAAAGGCTATTCATCATTATCAAATGATCTTACTAAATCAATTGGTGATAGCTTAGTTCAATATAAAGCTTCATTACAAAAAGTAATTGATGAAATTGATGATTTAGTTCAAAAAATCAATACCATAGGTTTTGTTCAAAGTGAATATGATACTGTTTATAACAAATATAAAGATGTACCAAATAAAGACGAAAACATCATTAAATTTATTGAAAATAAAGTATTTGTTGAAACTAAATTCTTAAACAAATATGACATCAAATTAGATGAAATAACTAATCAAGAATTAAAAGATAAAGTTACAACTAAATTTAATGAATTCAAGGCAGATATAGTTAACTTCTGTTCACAAAATATTACTAATTATGATGCTGATGTTGCAACACTTACAACAAAACATCAAGCAATTCAAAATAAATGAAATGCCGACAGTGAAATGATAAAAATTGGTAAATACCTTAAATATGTTGTTGAAGCAACAAACTTTGAAAAAGCTATTCAAGCAATTGAAAATTCATATAACAATGAACCTAGCGGATACAAAGATGTTGAAGCTTACAATAACTTCAAAAAAGAAGAACAAAACTTAGCTTCTGGAAAAACATCTATTGAAACAATACTTAACAATTTCAAAAACAAAAAACAAAGTGAATTAATTAGTGGAAATGTTCCTGAAGATGCTCAATGATATGAAACTGAAACAGCTAGAATTAAAGAATTAATGTCAAATAATAATCAAATAATAACAAATAAATTGCGTGAATTTGATGAACAAAATATTGAAAAATTAAGAAACTATGTTGAAGAACTTAGACAATTAGTAAATATTTATGAACATGCTTCAATAAAATTTGCAAGCACAAATTTAAATGACATTAATAAAGAAATTAGTGAAGTTGATGCTTTAATTGCAAATAGCAAAAAAGCCTATAAAACAGAAGATAAAGTAAAAAATAGCGCTGCAAACTTAAGTCAAAAAACACTTAATACTTACAATGAATTTAGAACTCAAACACTGAATAAATATAAACAATTAATGAGTCTAAATGTTACTCCTGTTGTAGCAAATGTTAGACCTTGATTATTATCTAGTGATCCTCAAAATGATATCATTGTAAACCAAAGAGAAATTGATGTATTAAACAATTTAAAAACAAATAAATATTCTTTAGATAAATTTAATGCTAATTATCCTGCTAATTTAGCAACTGTTAATAAACACAAAATAGGTGATTTATTAAGTCATGTTGAACAATTAATAAATGATTATTCTATTATTAAAGATGATATTGAAAATGGTGATTTTGAAGAACTTAAAAAATTAACTGAATTATATTATGAAGCAAGATATAAAGCTAAAGATGTTGAAACTAAACATAATAAAGAATACAAATTATTTGTTGAAAACTCATTTAATGGGCCAAATAAAGCTTTAGTTGAAATTCAAAATTCAATTGACATGCATAATCGTCAATATAAAGAATTAAACGAAAAATACTTCTTTGAAAGATTAAATTCATCAAATAATGCTAAAATGACTAGTTTATTAGTAGATAACTACCACAAATACTTTGTTGATTTAAATGGTAAATTACTAGGTCAAGTATCATTCAATAATGAAGATGAAAAAATTAATAAATATAGATGATATGCAAGTATTGAAACTCTATACAAACATGCTGAAACCTTTGGCAATGTAGCCCTAGCTCGATACATTAAATATTCAAATGTAATGGATAGTTTATATAGAGATTTAGGTAAAGATCCTGGAAATCGTAGAATGAGACATAACAATAACAAAATTGATTGAGAACGAAATATCTTAATGGGTTATGACATGGCTTTAGATGAAGAAATGCTTGCTATTAATGATTCAAATCAAAAATTTGATGATGGTTATAGAACAGTTATGTTAGTTGGTGGATTTGCTCAATATGTTAAAGATCATCTTAATGACAAAAATTCAATTTATGCAGCTAATAGTGGTTTACAATTATGATCATATATTTTTGATGAAAAAACTCAAACGCTTGACTTTACTGCTTTAAATTATGTAAATGAATTTAAATCAAAAAATCAAAACATTAAAGTTGCTAAATATGTTTTTGACTGAAATAGAGGCGAAAATGTTAAAAATAAAGAAGCTGTTAATAAATATTTTACAGATTCAACAGTTGCAAAAGAATTAAGCTATAGTGGTGTTGCTAAATATGGTGAATTATTTAACTTCAACAAAGTTATTATTCCAGATGGCAATTTATTTTCTCAACAAGTTGTAATTCCTTTATTAAGAGATGAAACAATATTTAAAGATGCTAAAGGTCAAAAAACAACAATTATTTTACCTAAAAACAACTTTAGATATAGTTATAGATTACAAACTTTATATAGTTTCTATGAAAATGAAAGTACAGTAGGAATTCCTTGTCCAGGTTATGCATATGATAAACGTTATAACACTCAATTTATTGAATTTGTAGCTGAAAATATGCAATTCATGGCTGATACTTTATACTCAGAAAATATGTATATGTGAAATGGAAATTGACAAAATAAAGGTATAAATCATCAATATTACTTCCCAATTTTCAAATATCATTTCTCAAAAACAAACAAAAGTACTTCATATGTAGATATCATGAATACATTCAAACATGTATTAGATTCTAGATATTCTCTTTTAGCAAATACTCCATATACTTATTGTTTAAATATTGAAAGTTTTGTTGCTTATAAAAATCACATAATTGATGATAATGCAAAAAAACAATATAACCAAATAGTTTCTAATATTACAAATAGAATGAATTCAGCTTCAAGCAAAGAACAAGAAGCTAAAAAAATATTAGAAGAAATAAACAATAAATTACAAACGTTAAAAAATAATCCTAATACGCACTATTTTGGAAATAAAAATGTAGTAGGTCGTTCAATTGATCAAAAGCCACATATTACATTTAATATTGCTATTTATGATGAAAATCAAAAACATGATTACTACATAACAAGTACAACTAATTATCGTTTCTTCTTAAAAGATGTTAGCAAAGATATTTATTAGAAATTTACTTGCACAATTTGTGCAAGTTTTTATTTATTTAAAATTAAGCTAAAATGGTATGTGAACCGGGACTGTAAAAAATTTTGTGTGATGTCCCATGAAGAATAATTTTTCTTCAAATTTTAAAAGCTTTGCTTCTTTAGTTTCAAAGAAGGTCTCTCCTTTAATTGAATAAAGGGGAGAGTGGAGAGGAATTAAGATTTCTTGCATAGCAAGATCTTTTTCCTATTTTAAAATTTTAATTTTAAAAATAAGATAATGTTTTTTAAGCATTATCTTATTTTGTTCCACAATACTATTAGGTGTTGACGCAAAGTCAATTCCTTTCTATTGAAAAATGGAGAGATTTTACACAAAATTCTAAGCAATTTCAATAATACTTTTTGTATGGAATAGTCATAATATTTTTTTAATTTAAATATTAAATGAACCCCCTTTTTTTTGAAAAAGGAATTTTGGGTTCAATATAATATTAAATATCATTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTGCAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGGCGATGATTTTTTCAAAATGTATTTCTCAGTGATTGTATATTCTGATTTTCAGTACATTTTATTTCATATGTAGTATGAAGCATCAAAGTTTCCTAGTGGGTTATCAAGAGTAAATTCAAAGATGTAATTTACTCATGAAATAGGAGCCCCGTTATGTTTTAATAAGAAATGAGCTGAAGAATGGTCTACTGTTCTTAAATATGAGAATAATTTATTAGAAAATGGCATATATCCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCAAGAAATGATCTATAGTGAGGACCGTTGAATCCATTTATTATTTCTTCATCTGGATTTTGTTTACCTGGAGTTTTAAAAGTTTTATATGGTTTTTCTTTGCTATAAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCGCTTCAACTTTCTCATCTTTCTTCATAATATCCATTGCTTGTTGGATTGTTGTATTTAATGAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGTCTTTAAAATCATTTTTAAGCATTATTTTAGAATCTGATATTTTTTTGATATTTAAAAGCATTTTTTCAGTAATTGTTTTCTCATATTCTATTAATTTGGCCAAAGTATATAGATCATCATGTCCACTTCAAATATCATCATGTATTGGTCCTGCATAATCAATATAAGATTTATTAGGATATTTTTGACTTATATATTTAACTGTTGGTTTACCCGGTTTACCAAGTTCTTGAACTTCAAAAATTGTATGTCAAGGGGCATAATGATCATTTTCAATTTGTTTACCTTGTTCGATTTCACTAGTAGTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTCTAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTACCTAGAACTTTAGGTTTAGTTCAAATTTTTGATTTTTCATCTCAATTTACATTATTTTGTGAGTCAAATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTGAACAGCTAATAAATAATCTGTTTCATTTAAATAATTTTTAATTAAATCAATGTTTGTAAAATCAACACCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTGACTATCTAATTTAGAAATTTGATTATTTTTAATTGCAATCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTCGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACACCAAATCATTTTTTAACAAAGTTAACTTTATTGTCTTTATCAGCTTGAACAATACTAAAGTTTTCTGTAGAATTAACATTTTTATAAGCTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCCACATTTGCTATTGCTGCTAATTTTGCATCCATTTCGTTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAATAAAATTATTGATTAAATTAATTTTTTCAAATAGTCCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTATTTTCAATTGAAGATATTTCTTTAGTTGATAAATTAGGTTTGTTATTATTAATATCTTCAATTATTTTTTTAACATCTTCGAATTGGTCTAATGTTTTTAAATTATCAGAAATTTTTGATAAGAAATCAGCAATTTTTGGATTCGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTCGAAGCAAAGTTATTGACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTCAAATATTTTTAAATAATTAGTTTCATATTCTTTAAGATTTGAAATAATTTCAAAGAATGTTGAAATATTATTAATTTTGTTGTCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTAGTTTGTATATCAATTATTGATTTATAAGAAGTTTCAAATAAATTAAATCCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTCAATCTTGATTTAATATTAACAAAGGCTTTTTTAAATCCATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATATTCATTAATTAATTTGTTTCTGCCTTCAATATACTTATCTCATTTGAGAGAACTTAATTCAATTTTAGATTTTTGTACAAATTGATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATTTAACTGTTGTTTCAATTGAATCGACATTTGAATTATAAGCATTTAAAAATTCTTTAATAACAGACTGAATAGAAGTTGTCAAATCATTTTCAACATTTAGAATAGCTTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAACAAATGAGTTAAATTCATTAAGACCATTAATAATAGGTGTTAATGATTTGTTGCTTAAGTCTAGAATTTGAGATTTATTAAATAATTTTGTATTTTCAATTGTTTTAACAAAAGTATTAACATAACGATTTATATATTCCTTCGTATTTCCATATGATTGTTCTAATGTTTTACATGCCTTAATAATATCTTCTATAATAACGCTTCTATTTACTAAAACACCGCCATTTTCTAAAATAACGTCATAATTATTAGAGTCAAATCTTGATAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTATCTATGTTTGACATGAATTTTTTAATTAAACCATCTTTTTTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCGGATTTAATAAAATCTGCTGCCTTATCAATCATTTCAGTTGAAATGTTAGGTTTTATGTTGCTTGAAAGTGCTTCTTCTTCAATTTTACTTAAAGTGTTTTGATATTCCTTAATTTTAAGTTCTAAAGAATTTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCAGTTTTTAAAGTTAATAAATCATTACTACGAACAACAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTATCATTTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTGTGTTTAACAGCTCTTATATAACTTTTGTTACCAAAGTGGTGACCAATATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAGTCAATTTGAGTTTGGCACAAACTTAGTTCATTTCAAAGACTTGAAAGTCATGCTTGATTTTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAGGTATTTTATATTGTTTTCAATTCACAATTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCAGCTGATACTGCACCAACAATTCCGGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAACATAAAAAAATTAAATTTTCCGGACATAAGGAAAATTATGCCTTAAAAATAACCATAAAAAGCACCAAAAAAGGTGAAAATTATTTTCTAAAAGCACAGATTATTTATTAATTTAATGGTAAAATATAAATATATTGACAGAGGTATTTACCTATAAAACAGGTGGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTTGAATTCAAGTGTTTTAATCCTTTTACTCTATCATGAAAGGATTTTATTTTGCAAAGAAACCGAATAGAAAACAAAAAATATCATTATTCATGATTATTTGATATTTATGTTAATAGTAAAAACGAAAGTTATAAAAAACTCAAAAGATGAGTTAAAGTAACTTTTATTTTGCTTATTCTAATAATTTGTTTATATTCATTATTATCTTTTGATTGAAAAATTATGGACAATAATTCAGTAAAACAATTAAAAAATGATCTACAAAGATTATTTACTTTTAATAATATAAACTCTTCATATCCCGCAAATAATTTATGAATTTTATCTTTAAGTTTTTTATGAAGTACCATTCAATATACTGCATTAGGAAGTCTGCTTGGTTTTCTTTTAGCTTTAGTAACAAGCTATTTAGCTTCTTGAAATTTACATAAAAATAAAATATTACCAATAATAAGTAAAATAATAATTACGCTATTAAGAACTTTACCAATATTCTTTTTTGTATTTCTTTTTACTTCAAGCTTTGATAGATTGCTTTCAGCTTCTTTGATATTGACTTGATTTACTTGACTTTGATTGCATAAATATTTATGTGAATTATGAGAAAACAGTAAAGTTAATTTTTATTGAAATTCAATTTTACTAGGACAAAATAAATTTGCTTCTTTTATTAATAATGTTTCTTATCGTAATAAGAATAAAGTAATAGTGTTATTTCTTTATTCATTTGAATCAAATATTAGATGAAGCAGTGTTTTAAGTACTTTAGGACTTTTTGGAATTGGAGAATTAATCAATAATCCTCTTAAAAGTGGTAACTATAGCATTATTGCAATTCCACTTCTTTATTTAGCACTAATACTATTAATATTAGAAATTTATAGTTTAATAATTAATCACTTAATCTTTAATCCACTTAAATTAGAAATTAACAATAAAATAAAAGGTTTAGTTCGAGTTAAACTATATTTAATAATTAGTTTTTCATTAATTTTTTTAACGAGTGTTATTACACTTATTAATATTAAATATCAATTTAATTTAGATATTTTTAAAAGTAGATGAAGTGAATTAACTTATAAAGCAAATTATTCAATTTTGAAAAGCGAAAGTTATTCTATTTTCAAAGAATTACTAATTACTATCAAAATTACTATTGCCAGCATTTTCTTAGCTTTTATCTTTTCAATTTTTGCTTCTTTTTTTGCAAACGATAAAACAAATAATAAATTTGTGAAAATAATAAGTAAAATGCTTTTAGCATTCTTTAGAACTATACCAATAACCTTATTTTTCTATTTATCAATTAATCTTTATTATTCAAGAGAAGTCATTTTAATTTTAGCTTTATCTTTTACTACAATGCGTTCAATGAGTAAATTTTATACTGAATCAATTAATAATATTGATCAAAAATTAATTGACAATTATTTATTATTAAATAAAAATAAAATAACTCTATATTTTGAATTTATTTTACCTAGAGTAATTAAAGATTTTTATAGTTATGCAGCTTTTAGATTCGAATTAATCTTTAGAAACATTATTAATTATGGAACTTTTGCAGCTGTTGGTTTAGGTGCTAGATTAAATTATTACCAAAACAAAAATGAATGACAAAAAGTTGGAGCTTTAATATTAATCTTTTGACTTTGTTTAATTTCCATCGAATTAATTACAACCTTAATCAAATATCGCAAAAACATAATTGTTTGATTGAAATTCAAAATAAAAAAGCCTAAACTAGTTAGACTTTAATATTGTTCTTGAAATTCTTTTTCGCTTATATTATTAGCTTGTTTAATAAAAACAAGTTTCTTATTTTTTAATAATAAAACATAATCCATATATTCTATTGAATTAGCAACATCATGAATGTTACATAATAATAATGCATTATTATCATGAACATATTTTTGCAAATATTTTAATATTTTTTTAGTATTAATAAAATCTAAATTACTTGTTGGTTCATCAGCCAAAATAATATTTTTATTTTCAAAAAAGATTGAGGCTAGTTCCACTCTTTGTTTTTGACCACCGCTAAGATCTTTAACTGGTGTATTAATATAATCAAAAATATCTAAATATTCTAAGATTTTTGCTAGCTTATTTTTTTGTTCTTTAGTTAATATTCTAAAAACTTTGTAAAATCAATTATTATAGATATTATTTGACTTTCTTTTAATATTTTCAATGACATCATCTGTCTCAATTAAAGAAGGCTCTTGAAACAAAAAAGAAATATCTTTTTTAACTGTTTTATAGGTCTTTTGATCAATTTTATTGATATCTTGATCATTAATTAATATTTGCCCTTTAGCTATTAATTTTGGTTCTAAAATAGCTTTAATCAAAGTACTTTTCCCAACCCCACTAAGCCCATAAATTCCATAAGTTTTGCCACTTTCAAAAATGATATTTAAATTGCTTAAGACCACTTCATTTTGATAAGCTAAAGTTACATTTTTAAATTCAATTTTGTATGTTTTCATATTATTGTTTCATAACTGCTTCTATTCTATTTATAAATTCTGTTTGTTCATTACTAATAACTGAATAATGATTTAGACCTCATAAATTTCCAACATAATTATTATTTTTATTTGCTAATTCAATATAAGTTTGAGCTAATAATTCTAATACATTTTTACTTAGTGAATTATTTGCTATAGCCACATTATAAGGAATAGGATCAGTTAAAGTTAACATTTCATAAGTTTTATTTTTATCTTTAACATAAAAATCTCTATTTTCTAATGCTTTATTTCCATGATTACTATAAGCAAAAGAATTCTCATTATCAAAAGCTATATGATAATTTGAAGAATTATCCTTACCCAATTCTTTAGCTTTTCCTTTTTTAAAATATTTGGAATTTGAACTTGCAAAAGAATCTAGTGTAAAGTTTTTGTCTAAATTAAATTGTTTTTTTAATAATGCTTGAGGTAAATGATATTTTGATGCACTATCATTACTTCCATAATAAATTCCTTTTTTATAAAAAGTTTCTCAATCTTTATCATTTCAAGCTTTTTTGATTAAATTTCTTTCTTCATTAGTACCATAAATTCAAATACATCCTCTTTGATGATCTACATATTTATCAGCTTCATACATATTTAAAAATTTATAATCAACAAAGCCGCCCATGCTTTTGTCACTTCAATTTACATAGCCACCTTCACTATTGAATAATTCATTTTCATATTGAGCTTGTAAAGTTGCGAAGTTATTACTTTTACTTGGATCATAAAGACTTTGATCATTGTAATTTAAACTACTTTTTGTTTTAAAAGCTCTAGTTTTTGTTTGCATAAAAGCTACAACATTTTTATTTTTATTATTCAAATATTCTTGATAATATCTTCAAGCTCCGGGAAAAGCCACTTGAAATTTGTTTAAATTAACTTCTTTAAGTGTTGCGCTTTCATCATTTCCTGAATCATGAAACGAAACTACTTTTTGATTTAAATTTGCTTCTTTCATTTTCATATTAAAAACTTGATTAAATACTTTTGTATATGCATCTAACTTTTCTTTATCTGCATATTTAATACCTTCCATTTTAATTTTTATTTCTTCAATATTAGGTTGATATTGGCAACCAGCACTAATACTTCCTAAAGCAAAAATACTTGAACTTGCTACAATAAATTTATATATTTTTTTCATAAAAAAATCCTTTCACGACAGAGTAAAAGGATTAGAGAAAAAACTATTTATAATTCAAATTAATTGAACTTGCTCCGCAGGTATTAACCTGATCAGCTCTAAGAGTTTTTCTCAATCTGCTTGTTATATAAGCAAATACCTCTGTCAATTTCATATTTAGTATAACACAAAAAAATAAAAATCTACTTATTTAGTAGATTCATGTTCTTCTTTTTCACTAATTAAAACAACTTCAGTATCATCTGAATTATCTTCGCTTTTTTTTTGGCCTAATTTACTTTCTGTATGATTAATTAAACGAGTAATATTACTATGATGCATTGCAATAATTAATATTGCAGCTATTAAATAGATAAAACTTGTTACAAAGCAATTGTGATATTCATACACAAAGTTTGTTCAATATCCTAAAATGCCACTAGTCATTCAAGGAATAAAAGCAAAGGGCACTAATATTGCCGCTGTTAATATTGAAGCTAAAGATACATATTTAGAAGTTAAGACAACTATAAAGAAGATAATAGAAGCCATTAATAAAAAGACAATATTAATTGTGGCAATTAAACCAATTGTACAAGCTACACCTTTACCACCTTTAAATTTAAAGTAAACTGGAAAAATATGTCCAATGATTACACCTAAAGCTAAAGATTGTGGGCTCATGTAATAAGTTTGAGCAAAGTCAAAACAAGCATGATTTAAAATTGCTGCTAAAACTATTGTTGGAATAAAAGTTTTTAAAACATCAATAACAAAAACTGTTGCTGCAAACTTTTTACCATAAGTCCTTAAGCTATTAGTCGCTCCCGCATTTTTTGAATTATGCAGTCTAATATCATCTTTTTTCTTTCATTTGGATAATATAATGCTTGTATTAAATGATCCAAGTAAAAAATATCCAATTAAGATTAAAGTTAAATTTAACAAAATACTATAAAAAATATTCATGAATTTATTTTACAATAAAAACAAATAATTTTTATTATTGCTAAAAAATATTTTTGATTAATCATAGAAAAGAGCTAAAGCAATGTTCACATATAAAAATGCTAAAAAAATTGGTTTTTTTATACTTACTATGCTAATAGGAACTGTTGTTGGTTTGGGATATTTTTTAAAAATTAATTAATAAAAAAGCTACTAGTGAAGATAATTTAAGTTGATTAATGACTTGAATTTTAGCGGGGTTAATTTAATTTCACTTTATAATGCTTTAAGTTTTAACAAAATTGACCAACTTAAAAATACTAAAACAAATGGTATCAGCAACTGAGCTACACAATTAGGTAATAAAAAATTTTGTTATTTCATCAGCTTTAACTATACATTTTTATCAGTTGGATCTACTGCTAATAACTTAAAGAAAGATAACTTACCAACTGTTTCTATTGTTGGAATGATTACAGTTATAATAAATTATTCTTTAATTGCTATTTTTGCAATTTTACATAATAGTGGAGTTGTTGAACAAATAATTAAAAATACAATTGATCAAAGAACAAATCAACATTTAGTGCAAAGTTTAAGCATTATTTTTAGTCTATTCTTATTTATTGCTGTTGTTGGAGTATTAAATGATTTTACCAATTCATATGCACATAATATTAATAATGCTATTAATTTAAAAATAATAATTAATACTACTAAAATAAGAAACAAAATGAATCTTAAAAGAATGATGATTTTAATTATAAGTCTTATTTATAGTGCTATTTGACTAATTATTTCAATTAGCATCTGAGTTTTAGGTGGCGATAACATATTAGATGGTTAAAGCAATTTTCCAACTTTTATCTTCTTCAATTTTTATGCTGTTATTATTGTTTTGTATTTACACAAAAAAACATTGAATATTAAAAAGATGAATACAGTTATATTCTGAATCTTTTCAATTTTTGCAATTGTAGGATCATTATTGGCAATTGTTGCTTATATAGGCGCAACAATTGCCGGAATAGTAAATAATGATAAAGCACTAGGCAGTATTTTCTATGGTAAAGACATCTCTATTAGTAAATGAGTTGAATTAGGCTTATATTTAGGATTTTGAGTATTAGTAATAGTAGCCCCAATAATTAATTATTATTTGATAAAAGCTATTGAAAAATGATATGTTTTAGAAGAATTTGATTTAGAAAATTATCAGTCAAAATATGTAGAGGATTTATGGAGCAATTGCAACTTTAATGACTCTCATGAAGTTATTAGAGTTTTAGAAAAAGAACCAGAAAATAATCTTCAAGAACAAAAAAGCGAGTAATTAAATGCTCATTTTTTTGTTCTTTTTATATTATTTATCACAAAAGTGTTCGGCAAATCACCACAAAAGTGTTCGGCAAATTATCACAAAAGTGTTCAGCAAATTACCACAAAAGTGTTCGGCAAATTATTTTTTTGTTATAATGTTTACGAGGTTACTATGAAATATAAAAGAAGAATAATAGAAAAACAAATAAAAAAAAATCTTGAAATTTTTGGTGCTATAAACATAGAAGGAATTAAACAATGTGGTAAAACAACTACTGCACAAAAATTTTCAAAAACATTAATTAAATTACAAGATCCAGACAATTTAAAAACTTACAAAGAAACAATTGAATATAAACCTTCATTACTTTTAGAAGGAGAAAAACCATTATTAATAGATGAATGACAAGACTTTCCAATTATGTGAGATGCCGTAAGAACTTATGTTGATTCAGCAATGGGAAAAAAGGGGTTATTTATTTTAACAGGCTCAAGTACACACAATAAATTAAATACTATGCATACTGGTACTGGTAGAATACATACAATGAAAATGTATCCAATGAGTTTATATGAAACTGGAGAATCTAGTGGAAAAATATCTTTACAAGATTTATTTGATAAAAAAATTAAAGTAAATGGTTTAAAATCAAATTTATCTTTTGAAGATTTAGCAAAAGCAATTTGCAGAGGAGGATGGCCAAGTACTCTAAATTTAAAAAAATATGAAAATCAAATCGAAATTCCTAAAGATTATGTCAATCAAATTTGCAATGTAGATATAAGTAAAATTGATAACATAAAAAGAAGCCCTGAAATTACTAGAAGAATATTGAAAAGTTATGCAAGAAATGTTGCTACTTTAGCTACTTCAAAAACTTTAATAAAAGATTGTAGTTTTAAAGATGAAGGTTCAAAAACAACTTTTTATGATTACACTACAAAACTTGAAGATTTAAATGTTATTGATAATATAACTGCATGAAACCCTAAAATTAGGTCAGCTTCAGCAATTAGAAGTACAAACAAGAGACTTTTTGTAGATCCTTCTTTAGCAACAAGTTCTTTAGGTATTTCATATATAGATTTAATAAAGGATTTACGAACATATGGTTTTGTATTTGAAAATTTGTGTTCTAGAGATTTAAAAATATATTCATCAAGTTTAAATGGAGAATTATCATATTATCACGATAGATATGGTCTTGAAATTGATTTTGTTTTGCACTTAAATGATGGCAGATATGCATTGATAGAATGTAAATTAGGTGGAAATCAAATTGAAAAAGCGAAAAATAATTTATTAAAAGTAAAACAGCTGATAAAAGAAAATGGACAAAGAGAACCGGATCTTATGATTATTTTAACAGCAGAAGATAGAGCGTATGAAATAACAGATGGAATACTTATTATTCCAATTAGTCTACTAAAAGATTAAAATAAAACTTCTCATTTATTGAGAAGTGCTTTTTATTTTTGACAATTTGGACAATAATAAGTCCCTCTTTTGTTAATAAAAATTTTATTAATTTTAGCTTTTTTACATCTTTTACATACATCATTAAAGTGTCCATGAACTTGTAAAAAATCTTGAAATTGACCTTTAACTCCATCAACTGAAGTATATGTGTCAATGCTTGAACCACCTAATTCTGTAGCTTGATCCATAATTTTTGCAGCTGCTTTAATAATTTTTTCTAACTGTTCAAAAGTTATATTTTTTGAAGGCGTCAAAGGATTAATTTGAGTGGCAAATAAAGTTTCATTAGCATAAATATTTCCAATACCTGAAACAAAGCTTTGATCCAAAAGAAGCTGTTTTATAGGTATATTCTTATTTTTAATTTTATTAAATAACTCTTTAATATTTGTTTCTGAAGGAATTTTTGCAACTTTATCTAAAGGTTTAGTTGTATATAAAGTGCCCTTAGTTTTTATATGAAAAGTGCCAAATTGACGTGAGTCATTATAATAAATACAACTATTATCATCAAGATGAAAAATTAAGTAATCATGTTTTGTTGCTGGACGATATTTATAATGTGTAAAATACTTTCCACTCATTCTCAAGTGGCTTAATAAATATTTATTATTTGTTAAAGAAAAAATAATATGTTTAGCTAAATTATTGACATCTAAAAATGTTTCATTAATTAAAAAATTCTTGAATTCTTCAACACTTACCTCTTTAATTAATTTAGGATGTAAGATATCAATTTTAACTATTTTTCTATTAACAACTTTAGGTCTTAAATCTTTGACCACACTTCTAACTTCTGGTAATTCTGGCATAATGTTTTAATTATATATTAAAAAAAGAATATAATTTAATATATATGCATTGGAGGTTAAATTATGGCTTGTTCAAAGAAAAATTGTTGCACAAAAAATTGTGAAGTAAAAGACTGTGCAACTAAATGTACAACAAAAGATTGCTGCAAAAATTCATGTTGTTCATCATGTTGCAAAGAAAAATGCCGCAAAGAATGCAAATGTTGTTAATTAAAAATAGAATATTGCTTTAATTAGCAATATTCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATTGCATGTCAATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCAATAGGACAAATAAAAGTGAGGGCATTAACCCTTTCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTGCTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAACAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAAAATTTCTTCTTCAGTAATATCATCAAAATTAGTCTTTTTCTTAAAAAAATCTTCTAACTAAATCATTGAAATTTTCATTTGAACTTCTTTGAAAAGAAGCATATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTAAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTTAAATTTCGTGGATTTTTATTAGGAATTTTTACCAAAAATCCATATCTAAAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTGCCTTTGCTCTTACCTACAATAAGGTCTATTTCCCAATGGCCAAATTCTTTTCTTTCATTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCGCCGACCAGTCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTTCGCCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATGACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATCAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTAACAGTCCGCATTTTACCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATATAAAGCAAAATAACCTCAAAAAGTAGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGTTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCTTTGGGTGACTTTTTTTATAAAAAAAACTTCACCCATTATAGATTCTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAATATTGCTTTAATTAGCAATATTCTTTTTTCTTAATTTTTTAACAATCCAATAGGCACAACATAAACATTATCTTCTCTTAAATAAGCACTTTTCCCTGTTCCTGTAATTACGATTAATTTTTTAGGTTTTGTTGCCTTTTGTTTTTCTAATTTGTTAGAAAATCTAACTAATTTTTCAGAAGCTTTATCAACTTGTTCTCAGCCTAATTTAATTTCAATTGCAACTCATTCGCCATTTTTTAATTCAATAACAGCATCTAATTCATTATCATTATAATCTTGATAATGATAAATATTTCACTCATTACTTTGAGCATAAACTTTCAAATCTCTTAAGACAAGGTTTTCAAATAAAAAACCCAAAGTATTTAAATCATTTTGCAAACTCTCATTATTAACATTCAATAACGCAGCTGCTAAAGAAGGATCGACAAAAAGTCTTTTTTCATTTTGTTTAATTCTTAAATTGGATCTAATATTCAAACTAAATGGTTGCAAATTATCAACAATAAAAGATCTATTTAAAATATCTAAATATGCATAAATAGTTTTTTTATCAATTGTTTCATTTTCATTTGTTTTAATATCATCAATTAGTTTATTAATGCTAACTGTTATCGCATTATTTCGAGCTAATGATTTTAATAATTTTTGTAATTTATTTTTATCAAATTTAGTATCACTAATTTTATCTAAATCTTCAGTTAAAAAAGTGTTTACATAATTACTTGCCGCTAAATAAGAGTATTTATCATCAACTTCAATATTGTCAGGTCAACCACCTCTAATAATTAAATTAATAATTTTATTTAATTCAATACCTTCATAAGTTGTATTTTCAAAATTGTCATTTATTAAATCTTTTAATGAAATTTGTCCTAAAGAATCTTTAGATTCAAATAAGCTCATAGGTCTCAATTGTAATGTAGCAATTCGACCAATACCACTATGAAATAGGCCTTTATGTTCAGGAGTAGAAGAACCAGTCAAAATGAATTGACCTTTAAGTCCACGGTTATCTACTTCGAATTTAACTACATCTCAAATAGGTGGATATTCTTGTCATTCGTCAATTAGTCTTGGTGTTTTTCCTTCTAAAATTTTAGTTGGATTTATTTTTGCTAAATGTCTATTTTCAAAATTATTTCTAGAATCTCCAACATAAAATGAACTGCTTGAGTGAAATTGTGATGTTCATGTTTTGCCACATCATTTAGGTCCTTGAATGCAAATTGCTCCAAAAACTTTTAAATATTTTCTTATTATTGGATCTAATAATCTTTCATAATACATTTCTTTTTTATATGTCATAAATATTTCTTTTTATTTATTAATATTATATATTAAAAGTTATTAGAGTCTATTAAAAATTCCTAAAATGGTGGAACACATTCCACCAAATTCAGTGGAATGTGTTCCACCATTTTAGGCTTTTTGAATAAAATTTAGAATAAAAATAAGCCTTTTAACAAGACTTATAATTTAAATTTTTATTATTTAAGAATTTTAAACATATTAGCTTTATAAACTTCAACACCTGGTTGGTTGAATGGATTAACACCTAAAAGATAAGCACTAATAGCTAAAGCTCTTTCAAAGAATTGGAATAAGTAACCTAATGTGTATTCATCAAATTTTGCAAAATTCATTACAATGTTTGGAACTTTACCTGTGTTCATGTGAGCATCTAAAGTTGCTGTAAAAGCTGTCATGTTAACATTATGCAATTTGTTTTTATCTAAATAAACAAGTTTATCTAAATCTTCATTATCAGCTTTAAAAGTAATATCGTATTTTGGTTTATCTAAAACTAAAATTGTTTCAAATAATACTTTATTTCCATCTTGAATCATTTGACCAAGTGAGTGCAAGTCTGTTGAAAAAATTCCGCTTGTTGGGAATAATCCTTTGCCATCTTTCCCTTCACTTTCAGCAAATAATTGTTTTCATCATTCTGCAAAATATTGCATTTTTGGCTCATAAGAAATCATCATTTCCATGGTGTAATTTTTACTTAAAACATGTCTTGCAACCGCATAACGATAAGCATCATTTGTTTTTAAACTTAAATCATTTGCATCTTTATTAGCTTCTTGAGCACCTTTAAGAATTTGTTCAATATCAATTCCCGCACAAATGAATGGGAATAAACCAACTGGACTTAAAACACTAAAACGTCCACCAACATCATCAGGAATAATTAATTTTTCATATCCTTTTTGAGTTGCTAATGAGTATAAAACGCCTTTTTTAGCATCAGTAGTAGCCACAATTAAATTCTTAGCTTCTACTGCTCCAACTTTTGCTTCTAATAATTTTCTAAATTCTCTAAAAGCAATAGAAGGTTCTAAAGTTGTTCCACTTTTTGAAATAACATTAATTGCAAACTTTTTGTTTTCAACATATTTTAATGCTGCTACTAAGCTTTCACTACTAATGCTATTTCCAGCAAATAATAATTCCATTTTTGGTTTGTTAATGCTGTAAGGTCCATACATAAATTCATAGCCACTTTTAGCTCCTAAATATGACCCACCAATACCAATAACTACAACTACTTCAATGTTTTTGCTTTCTCATAGTTCAGCTATCTTTTTCATTCTTTTAACTTCTTCACTTTTGCCTTTGTTAGGTAATTCTAATCAACCTAATCAATCTTTTTCTGCAATAGTTTTGTTCATTATTGAATCATGAATTTGTTTAACTTTGTCTTGATAAGAAAGTAATGTTTTTTCATCAACACAATTATGTAAATTAACTTTAACTGCTTTCATATTTTTTTCTCCTTAACTTAATAATTTCTTTAATTATTCTACATTTTTAATATTTTCTTTTGTTAACTATCTTCTTTTAATTAAAAAATATGGAAATTATTACAATTTTTATATATCAAAAGGATTCAATTTATTTAAGTAACATTCAAAAATATAACTTCAATATAGAAATCCAACTAAGTATCCTAAATTTAATTCATTTGAACTTTCCAATGAAATATCAAAAACTGGTAATGATGCAACATTTGAAAGTGTTGTCAAATAACCTTTTTTTGCTACTTTGTAATAGCTGCTTATGCGGTTATCTTTATGGTTCTCTAAACCATCTTGAGTATTATTAATTTCATCACTAATTCTAAAGTCAATTTTTTCATTTTCAATGCTAAAAATTGAGGTAAAAAAGTTGCGATTATTATCAATTAAATATTGTCCATAAGTATAAATATCTTGACTAAAAATTGCATACATTGTCACATTATTTGACTTTAAATATTGACTATTATTTATTGTGCTATAAAGCTTCAAAATACCTTTAAATCTTTCATCAGTTCCAATTAAAAGATCATTTTTTCGATCACCTTTTAAGCTATACCTAATGTAGGCATATTGGAATGCTTTGTTTTGTTCTAAGTTACTACTTAATCAGTTTGTGCAACCTGAAGAATAGCCTTCAGATAAGTCATTAATATTAGCTCCTTGAAGCAACAAAAGTAACATATTAATTTCACTAAAAAAAGCAAAATTATTTGAGAGGATATTTGGACTAATAAGTCTGTTTTGAAGGGGAATACTTAAAAATTCCAATTGTTTTTCTAATGATTCTTTTGAGATTAAAAAAACTCTTTCAAGTGCTCGATAATAACCTTGATTTAATTGAAAATGATTCATTAATAATTTAATAAAATTAATTGTTTCTTCACTAAATTTAGTAAATGATCCAAACATTAAAGCAATGCCTTTTTGTTCAAATTTTTTCTTATACTCTTCAAACTTAGCATAGTAATCTTGCTCATAATTATCAACATTAATAAAATTAAATTTTAACTTGTATTTTTTTAATAAATCATACTTAAATAAAAAGTCATTTCCAGCTTGATAATTATCAATTATTTTGCTTGTTGTCATAATCAAAATTTCTTTAATTTCTTGCTTTTTTATTAAGCGTACAAAATTGTTTAATTCATCAACATTAAAATTACTAAAATTTAAAGCTAATTCATGAAAACCAAAATGTTCAAAACCCTCGATATTTTTTAATTTAACATCCTTAATCAAGTTATTGCCACGATTAATCAAACTTTCATTTTTAAAATCAGGTGAATAATTATAAGCTTTCAAATTAATTGTTGCCATTATTACCTTCTTTATGATAATTTTTGATTTCTTGATCAATTTGGTTTTTCAAGTTTTCAAAACCATGTTTTGTATTGCTTAAATTTTCTTTAAATGGACTGCGTGAAAAGGTGGCATGATTAATTTTAAAATTACCAATACCACTATGACTGTGATAATCAACTTTTTCAACTATAAAATTAATTTTTTCGCCGATCTTAAATTCATGACGCAAGTTAATTTTGTTTCAGTCAGTTATCAAATCACGAGGAATAAAATAAGAATAATCATTATTGCATAAAATTGTAAGTCCTTCACTACTTACACTTTTTATTTTTCCGAATAAAATATCGCCTTTATGATTCATGGTGTTTTTATTATACTTAAACTTTATGTTTATATTAATAATTAAATGGAAAAAAATAAGAAAAAATGCAAAAATATTTGATTTTTTAGCAATTTTTTTGGTCAAAAATAAAAAATATTTATTTTTAGCTAATTTTTTTGAACTAATTGCATTATAAAATTAAATATTAATTTATAATATTAATACAATAATAAAATACTATTGTTTGAGGCCAAAAATAGTAAAAAAAATTTTCAATAAATACACTATTTATTGGCTAATTAGTAAAAAATAAGCGAGGAATCAATATGAAAAGACTTAACTGTACATATGAAGTTAAAAAGGACCCAATTTATCCTTGATTACTAAAACATCCAAAAATCAAAATTGGATTAGCAAAATTTAAAACAAGACAAGAAGCTCTTGAATGATACATGTTACTTAAGTATGAAACAGCTGTTTGATATCAAAATGATCAAAAGATTTTTGAAGGTCAATTAACTTGTGATTGTGAAGATGGACAATGAAGCTACTACATTAAAACTGCTGGTTTTGATGGTGGCGGTACATATGAAGGTAACTGTCGTCAAATTGGAATTAATCCTTCAACATTCCAACACGATGATTATGATGCAAAAAGAAGATTAAGAAATCTTGATTTTGTATTAATTTCTGATCCAGCAACCTACTTTCCACCTGAATTAGAAATTGCTAAAAAGAAAAAGAAATCAGATGTTGTTGACGTTGATGCTATTAGAGCACAATTCCAAGAACAAATTAATATTCTTTTAACTCAAATTCATGAAAACAATGCTGAAGCAGACAAAGAACTTGAACTTCTTAAACAAGAATTAGCAAAAAAAGATATGAACTTCAATGAAATGGCTCGTAAAATGGAACTTTTAAGACAAAACTACAAGCCACTTGAAGGTGTAACATACATGAACTACATCACATTAAGTTTTGATGACTATGTAGGTGGTGTTGCTTTATACACAGAAAAAATTAAAAATATTATTGAAACAACTCCAAAACAAAATATTAGTGAAGCTGATTACAATAATATTAAGAAAAACTATGCTAACTTCAATAAACAATTATTAGAAGTTGAAGCAAATCCTAACTTCCCACATGCTGATTTAGTTCAAAAAATGCATGCTGAATTAAGTACAACTTTTGAATCATTACTTAGTTTATTAGTCTTAGATCCTAAATTAGAAGATACACCAGCTAACCGCGGTTATTTCCTAAATAAAGAAACTAAGCAAAAACAAGAAATCTCATGAGCTACTTCATATGTTTTAGTTGATCTTTATCATGTTGGATTTGTTCCTTATGAAGAATATGAATATGCAATTCCATATTTAGCAATTAGAAACAAATTTGCTGTAACAGTTATTAATGAATCAGATGCTACTAAAACAATAACAATTCAATCTGCAGAAAGAGATGCAGTTGAAATGGAAGAAGCTGTTTTAGCTGCTGAAAAATCTTCTAAAACTAAAAAACAACCTAAAACTGAAACTATAGTTACTGAAGTGCCTCCAGTAGTTGAAGAAGTTAAAGATGAATCAGTTGTTGAAGCTGAAGCTGTAGTTGAAGAAGTTAAACCAGTTGAAGAAAAACAACCAACAATTAAAGAAGAACCGCTTATTGTTTTAGCTCCTGAAGAAGCTAAAAAAGAAAAAGTTGAAGACAATGATTTTGTCTTAATTGACCATAAAAAAGCTGAAAAACCTAAAACAAGAGACTTTGCTGTTAGAGTTGTTGAAGAAAAACCTATTCAAGTAATTGAAGAAAAAGAAGCTCAACCTGAAGATAATTTCTACACAGGAATTAGAGGTAATGAACCAGTTGCTCTAGAATCAGAAGAATCATCAAACAATGTTTCAACTAAGAAAAAACGTTCAGCAGGATTCTACTTCTTAATCTTATTATTAGTACTAATTCTTGCAGGTCTTTTAACCTTAGATATTCTTGCTATTATTCAATTAGCAGGTGGTTATACATTCTTTAATTTTGGTGCTTAATTTAATTATCAAGAAATGCACTTAGATGCATTTTTTGTAATTATTTACTATTATTCAATTAATAAAAGGAGAGATATTTATGAAGAATAATGAAAAAAATATGACTACTTTAATAAATCATTTAAAAGCTAGTGGATTTGTTTATCAAGGTAGTGAAATTTATGGTGGTTTAGCTAATACTTGAGACTATGGTCCTCTTGGTTCACTTTTAAAGGATAATATTGAAGCTGCTTGAAAAAGCGAATTTATTAAAAAAGAAAAAAACAACCATTTACTAGATTCAAAAATTTTAATGAATCCTCAAGTTTGAGTAACTTCAGGTCATGTTATTAACTTTAATGATCCTTTAATTGAAAACAAAGTTAATGGCAAAAGATATCGTGCTGACAAATTAGTTCAAGAGATTGATCCTAACATTATTCCAGAAAAAATGACTAATGATGAAATCAAAAAATTCCTTGAAAAGAATCTTAAAGAATACGATGGTGCTAAAACTAACTGAAGCGATATTAGAAAATTTAACTTAATGTTTGAAACCAAACAAGGAGTTACAGAAGAAACCAAAAACAAAATTTACTTACGTCCTGAAACTGCTCAAGGTATTTTTGTTAACTTTAAAAACGTACAAAGAAGCATGCGTTCAAAATTACCTATGGGCATAGGGCAAGTTGGAAAAAGTTTCAGAAATGAAGTAACACCAGGTAACTTTATTTTCCGTACTCGTGAATTTGAACAAATGGAACTTGAAGTATTTTGCCATCCAAAAGAAGCAAACAAACTTTACAAATACTACATTAAAAAAGCTGAAAAATTTGCTGAATATTTAGGCTTAAGCAAAGAAAATGTTCGTATCAGAGAACATGAAAAAGAAGAACTAAGTCATTATTCTTCAGGCACAAGTGACATTGAATATCTTTTCCCTTTTGGCTGAGGTGAACTTTTAGGAATAGCTAATCGTACTGACTATGATTTAAATGCTCATATGAAAGCTACTGGTGAAAACTTAGAATATGTTGACCCTGTAACAAACGAAAAATTTGTTCCTTTTGTTATTGAACCTTCAATAGGACTTGACCGTTTAATGTTAGCAATTTTAATTGATTCATATGACGAAGAAAAACTTGAAAAAGATGACTCAAGAGTTGTTTTACATCTACACAAAAATTTAGCTCCTTATCAAGTTGCAGTTTTACCTTTAGTTAAAAAACTAAATGATAAAGCAACAGAAGTTTATGAAAAATTACTAAAAGCAGGCTTATCTGTAACTTATGATGAAACTGGATCAATAGGCAAAAGATACCGTCGTCAAGATGCTATTGGTACACCTTATTGTGTTACAATAGACTTTGATTCACTTGAAAAAAATACTGTAACAATTAGAGACAGAGACAGTATGAAACAAAAAAACTGTACTAATTAATAATTTACTAAAAGAACTATAAAACAATAGTTTTAGAAAAAAACGGAGGTAACAAATGAGTAATAATAACCATGAAATAGTTGACTCAATTATTGCAAGTAATGATATTGTTGATGTTATCAACTCATTTGTTACTTTAAGCAAAAAAGGTCGTAGTTTTGTTTGCATTTGTCCTTTTCACCAAGACACAAATCCATCAATGAATGTCGACCCTAAAAAGCAAATATTTAAATGCTTTGTCTGCCAAGTTGGTGGTAATGTTTTAACATTTTTAATGAAATACAAAAAATGAACTTTCCAAGAATCACTAGAATATTTAGCTAATCGTAGTAATATCAATGTTGACTTAAGTTCATTCCAAAAATCAATAAGTCGTTACAATGAAGAAGACTTAAAAATAATTGAAATCAACAACAAAGCTAATTCACTTTTTAAACTAGAATTAATAAAAAGAAAAAACAAAGAATTAAAAGATTTTATTCATAAAAGAGCATTAACTAGTGAAATCATTCAGGCTTTTGATATTGGTTATAGTGAAGAAGAAAGTTTCAATTCAATTTTTGCAAATGAGTTAGAAAATATTCCTGACTTATTAGTCAAAGCAAGTTTAATTAATAGTCATAACAAAAGTGTCGCATTTAAGAATAGAATTACTTTTGCTATTAAAGATGAACAAGGTGAAATAGTTGGATTTAGTGCTCGTGCTTTAGACAATGATACTAAACCTAAATATATCAATAGTAATGAAACTAAACTTTTCAAAAAGAATTCTCTTTTATATAACTATTGACAAGCTAATAGTAAAAGCGAAGACAATTCAATCATAATTACTGAAGGATTTTTTGATGTTATTGCACTTTTTAAAGCTGAAATCTATAATGCTGTTGGTTTAATGGGTACAGCTTTAACCAATAATCATTTCCGATTACTAAAAAACAAAAAAGTAGTCATCTTTTTAGATGGTGATGATGCTGGTCAAAAAGCTTCACTAAAAAGTGCTAAGTTTTTATTAGACCATCGTATTGACACTTACATAGTACGTAACACAACTAAATATGACCCCGATGAATTATTTAATAGTTATGGTGCCGATATTCTTCACAAGTTAATTAAAGAAGCTCCAGCTGCATTAGATTTTATTTATGAAAATTTAAGCAAGAAATATAATCTCAGTGTTTCGCCAAACAATGAAATCAAAAATGTTAAAGCTTTTTGCAATGACTTTGTTAACTTCCTTGAACACCAAGAAATTAATGTTCAAGAATTCTACAAAAACAAAATTCAAACCGATTATGGTTTTAGCATTCAAGTAAAACACAACTTTAAGTTGCCTGATCCTTATGATTCTCAAGAAATTTATGCACCACAACAATTTCCAGAAGACTCAGGTGAATATCAATTATTAGAAAACAATATTCCCCCTTATGCTTATGATGAACAAAATTATAATCAAGTAGTTGAATACAGCAATAATGTCAATAATTTTAATCACCCTTTAAATAACGAAGTGCCATTAAGCGATTATTGAATTAACAATCTTTTCTATTTATTACTTAATCACACTGAATTAATTAAGCTATTTAAAGAAAGAGAAGATACACACAAAGACTTAATGTTTTTCGGTAAATTAAATAATGAAGATTTAAAAAAAGAACTTTATCAAACATTTAGAGAAAGTAAAAGTATTAAGGTTGCTAAATTAAAGGAATATATCAAAGACTTAGAACAGGAAAATGTTGATATCGATTTAAATCAATTTTCAAAAAAATACAAAGAGGATAAAATTATTAAACAAAACTTTAAAGATGTTTATCGCCGAGCATTAAAGGAATCAGATAATGCTTATAATAAATACACAAGCAATCCTGAAGTTTTGAAACAAATCAGCAGTATCAAAAAAATGTATGATGAACACAAAGAAAAATTAAGAGAAATGCAAAAAAGAAAGGAAGAAAATGAATAAAGCAAATGAAATTAAATCAATAATCTCAATGATTAAAAAACATGCAAAAAGCTTAAAGAAAAAACAAATAAGTCAGTCTAAAGTTTATGATTATTTAGATTCAATTAAAGTTGAAATTGATGATGAATCAATGGACGAAGTTTTAGGCCAACTTTTTGAATCAGGCTTAATTCTTGATGAAAGCGACGAGGGCGATTTAGACGATGTTAGTTTAGATGAATTAGACTCAGAATTAGAATTCGAAGAAAAAAGTAAAAAAGACGAAAACACCGATGAAATGGATGTTAAAAACAAAAAAAGTGATTTATATGACGAAGATTTAGATGAAGATGATAACTACAATTTAGTTGGTTCATATGGTGACGATGATGAAAGTCAAGAATACATTATTGAAGATGACTATGTTGATTCAAGTTATGATGAAGATGACGAAGATGAAGACGAAGAAGAATTAAGCAGTAAGAAAAAATCGAAAAAAAGTAAAAATAATGAAGATGAAGATTCACTTGAACTTGAATCTTATGAAAATGAAGAAATTAACCTTAATGATAACAAAGTAATTAAAGATAAAGATTTAACCAACAAACTTACCGAAACTAATGATATTGTTAAATGATACATGCGTTGAATTGGTAAATATGGAAAACTTTTAACCATTGAAGAAGAAAAGAAACTTGCTCAAGAAATGGAAAAAGGTGGCTTTAGAGGAAAACGTGCTAGGGATAAACTTATCAAACGTAATTTACGTTTAGTTATCAATAATGCTAAAAAATACAAAAATAGAGGACTTAGCTTTATTGACTTAATCTCAGAAGGTAACTCAGGTATCGTAAAAGCAGTTTCAAAATATAACGTTTCAAAAGGCTTTAAATTCTCAACATATGCAACTTGATGAATTCGTCAAGCAATAACTCGCGCTGTAGCTGATCAAGCTAGAACTATCAGAGTACCTGTTCACATGGTTGAAACTATTAACAAAATCACTAAAATTGAACGTGAATTGCAACAAGAATTAGGTTATGAACCAACTGATGAAATGATTGCTGAAAGATATGGCAATGGCTATGACGCTGAAAAAGTTAGATACATTAGAAAAATCAACATTGACCCTATTTCTTTAGACAAACAAATTGGTAAAGAAAATGATTCATCATTTAGTGACTTTGTTAAAGATGAAAGTGTTGTTAATCCAGTTGATTATGCTTCACAAGAAGAATTAGTGGAAACACTAAATTCAATTCTTGAAAAATCACTTGATGATGATGAAAGAATTTTAATCTGCAAACGTTTCGGAGTTGGAGTTGACGAAAATGGAAATAAATACCCAATTCACTCACTTGAAAAATTAGCAGAAGAAAGAAATGTTTCTAAAGAAAGAATTCGTCAAATTGAAAACAAAATTTTAAGAAAACTTAAAAGTAGCACACGTAAAGGACGTCACTTGAAAGATTTCATTAAATAATGCAAATTAGAAAAGTAACAAAATATTTATTAGAAAAGTGACCACTTGATCTTAAAGAAGAATGAGACCCAAGTGGTTTTTCTATTAAATTCAATTTATCAGAACCTTTACGTAGTATAGTTTGTGCTATTGACTTAACCAAAGAAGTTTTCGAAAAAGCTTTAGAAATAGATGCTAATTTAATCATCATTCATCACCCTTTTAAATTTGAAGCATCTTGAGATGATGAAGATATCAAAGCTCCATACAAAAGATCAATTTATCAAAAATGCAAAGAAAAAAGAATTAATGTTTTAGCTTTTCATACTAACTATGACAACCAATACTATGGAACTTCATATCAAATCATAAAAAAATTAGGACTTGAAAATTATCGCCAAAATTATGAACATAATTATCCTAATTTAGTAAATTACAAAACTACTTTTAATGATTTAGTTAAAAAAATTAAAACAACTTTTGGCTTCAAAGCTATGCGTACAAATTTACATAAAGACCTATGAAACAATGAAATTAGTAAAATTGCTTTCCTTTCAGGGGCTGGATATATTTCAGAAGTTAATGCTATAAATAATTTAAATTATGATCTTATTATTACAAGCGATATTAAATGAAGTGACTGAATAGTTTATAAAGAAACCAAAGCCAATATTTTAGAAATTTCTCACCTTGATGAACAAGTTTTCGCAGATGATGCATATAAAATAATCAAAGAAAAATTCCCTGGAGTTAAAATTGAATTAGTTCATATTAATGAACCATATTGAAACATTTAACAAAATATTTCTTCAAAATTAAAATTTATTTTTTATAATACTAAGGTAATAAAATTACATTTTTAAAGGAGAAAAATGCATAGAAAAAATCCTAAAAGTTCAGCTATTGCTTTGATTGTTTTATCACTTTTAAGCGCTGTTATAACAGGAATTGCAATATTTATGTTTATTGATGTTGCAGTTAAAATATTTAAAAGTGGAGAAGAAACAAATGAATATATTAAAAATAACTGAAAAGAAATTATTATCCCTACTGCATTAATTTTATCAATACTTCCTATAAGCGTTGCTTCATTTGTATTACATATTGTTATAACTATTCAAGCAAGCAAATTAGAAGATAAAAAATCAATGATAATCTTAATTATAGGATTCTTCGTTGGAATATTAACATTCATCGGAGCATTTATTTATATTTCTGATGCTCAAAAAGAAAATAAAAACTACTTCAATTATTTTAAATCAGATGAATCAATTGACTCAAAAGCAGAATAGAATTAATTTTAAAGACATTCTTATGTGAGTGTCTTTTTTTAAAAAATTTTTTTATTTTGTTTAAATTTATATAAAATTAAATTGCATCGGAAAATAGATGTTTTTGAGTTTAAATGAAAAGGAGATCTATGTCAACAGAAAACAAAACAGAATCTTTAAACAAAGTAAACAATGAAAAATTGGAAGCAAAAGAATTAAGCTATACTAAATGAATAGTTATTTGCAGTATTATTGCATGTAGCTTTATTGGATTTTTAATTCTTTTAGCTATTATTCAAAGTATTGTTATTGCGACTAATGGTTATACTCGATCATCTGCACTAACTTTTTCATTATTTACACTTATTGTTGGATTAGCACTAGGTGCAGTTTGAGTTACAATAACAGTATTTTCAATTTTCTTAGCAATTTACACTTCAGAATGAAATGATAAGACATTTATGATTTTATTTATAATTGGTTGCTTTATTTGAATTGTTTCCTTTGTTGCTGCTATACTACTTAGAAAAAAAGCAAATGTACAATTCTTCAAAGATGAAGTTATTGTAGTTAAAAAAGAAGTAGAAAAACAAGAAGAAAAATAATAATAAAATTATATAATATATGAGCCAAATTAAGGCTCATTTTTATATCAAAAGGATACTTATGAAACCAGTAGAAAATAAAAAACAATGATTATCAAGAGGTTATGTTGATCAAGAAGATATTGCAGAATTTGAAAACAAAAGCAAAAAAGAATTACTAGAATTATTGCAACATGAATATGCATGAGTTAGATCTTCTGCGGCTTATAATTTATCTCCTATGGATGAAGAAAGTGTAGATGCATTATTAAAACGATTAGAAAAAGAAGACAAACTTTATACTAAAATCGCTATTTGTGAAACTTTAGAAACAGGTAATGCTTTTACAGCTAATAAAATGATTGAATATTTGAGCAAAATTGGTAATAACCAAATTAAAGAATTGCCTAAATATATTAGTAATAAAAGTTCTTATCCACTACCAAGAGACATTATTGCCAAATCTTTAGCTAAAATGCATACTTCAATTTTTCCTCTTTTACTTGAAACTCTTAAGCAAGGAACAATTGAACAAAAACAAGAAGTTATTGATGCAATTGGTTACATTTGTTTTAATAAAAAAGAATTAGCTACTGAAAAACTTTTAAATGAGCTAATTGATGTTTATGGTCAAAATATTGATAATAAAATTTTTGTTTGGAAATTTTTAACATTATTATCAGCTTTTAATTTTGAAGCAAGTAAAGAAATACTTAACTCATATTTAAATAGCAAAGATACTTTATCAAAAGAAGCAACTCACTCTTTAGATATATTAAAGGAAGCAAATATTTAGCTAAATAATAATTTAGCTTTTTTATTGCGCTTTTAAACTTGTTGTATAATTGAAACATGAATTTAACAAAAATATTTACAATGCAAAAAGATCTGACAAAGCTATTGCAGCTCGTAATGATATTGATGCAAAGACTTCAAAAAGCAAAATTAAAGAATTTCACTACATGAAATTATTAGCTTTACTTGTTGAAATGGCAGAATTTGCAAATGAAGTCCAAACATTCAAATATTGAAAAGCAAAAAAACAAATTGATAAAGACAAAGCTTTAGAAGAATTTGCTGATATATTGCACTTTGTTGGCTCTTTTGCTTATCAATATGAAGTTGATCCTAACATCACACCATTAATAATGAGTAAGGATGTTAATAAACAAATTTGCTTATTATTCCAAACAATTTCTTCAGCAACAAATAACATCAATAAATATGTTATTGGAGAAATATTAGCTTTAACTTTAGGGGTTGCTAAATTATTAGGTTACAGTCAAAAAGATCTTTTAGAAGCTTATTACAACAAAAACAAAAAGAATTACGAACGCATTAAAAATAAATATTAGAAGCATTGTTACATGCTTCTATTTTTTATGGATTTTTTATAAATCTGTAATTATTTTGTCTCTTTTTTTAAGTTTTAATTCAATGTAAATAATGATTGAAAAGAAACTCAAAAATCAAATTGCAAAAATTATTATGAATAAATAATGATAATCTTTAAGTGAAAAAACAATGCTGCAAGAAATAAAAGACAATATTAAAATGACTATACTTTGAGAAAACAAACCTCTTCCAAAAGTGTGATTTGCATAATAAAAGTATTCATGATTTTTTAATGATAATTTTGTTCTATAGCTAAAAACTTTATTAAGCTTAGGAGTTTTTCAATAAAAATTTGTTCCAAAAATTGTTCCGATAATAGCTAATAATAAAGTTATTGATAATAAAACATAACTTATAATTTGCATTTCTTTCATCTTAAATATTATACATTAGTTGTTACTTTTTAAAAAATATGAGGGTATTAAAAAAAACTTATATTCAACGAATATAAGTCATTTAAATTATTTCTTATTTGCCTGTTTTATAGACTTTTTAGTCGAATCGCTTTTATCAGTTGTAGGCTTTTGATCTTTAAAAATGTGATTATAAATTTCTTCATAAGTTTCAACTGGAATAAAGGTAATTGCTTTTTTAACTTCTTCAGGAATATCTGCTAAATTCTTTTCATTGTCTTTTGGAATAAATACAGTTTTGATTCCTTTTTTATATGCAGCAAATGATTTTTCTTTTAATCCACCAATTTCAAAAACACGACCTCTTAAAGTAATTTCACCTGTCATTGCAATATCTTGAGGTACAGGTTTATTACTTAAAGCACTAACTAAAGCAGTTGTAAATGTTACACCAGCACTAGGCCCATCTTTAGGAACAGCTCCTTCTGGAACGTGAATATGAATTTCTCTTTCATCAAAAGCAAAATCTTTAATATCAAATTTTTCAGCATTAGCTCTTACATATGAAAGAGCAATATTGGCTGATTCCTTCATAACATCTTTAAGCGAACCGGTTAATTTGATACCGCTATTTTTACTCTTGAAGCTTGAAACTTCGATTTGCAATGTTGTTCCACCTATTGAAGTGTAAGCTAAACCGTTAACAACTCCAACTTGAGGTTCTTTCGTATCTTGGTGATCTGTAAATTTAGGAGTACCTAACATTTCAGTAACAATATCTTTTGTTATTGTAAATTTAGCAATTTTTTCACCTTCAACAATTCTTGTAACTATTTTACGAGCAATTTTATCTAAATCTCTCTTAAGTCCACGAACACCAGCTTCAGCTGTATAGTGTTTGATAATAAATTCTAGTGTTTTATCATCAATATTGAATTGTTCTTTTTTAAGACCTGCTTGTTGAATTGTAGAATCAATTAAGTGTTCACGTGCAATTTTAATTTTTTCATTAATTGTATATGAGTTTAATTCGATAATTTCCACACGGTCAATTAAAGGAGCAGGAATATTTTCGTAGTAGTTAGCAGTTGCAATAAACATAACTTTTGATAAGTCATATTCATGTTCAAGATAGTTATCTTGGAATTTTGTGTTTTGTTCAGGATCTAATACTTCAAGCATTGCACTAGCTGGATCACCTTTATAATCAGATGACATTTTGTCAATTTCATCCAATAAAATAAGTGGGTTAGATACACCAGCTCTTTGGATAGCTTTAATAATTTTACCTGGCATTGAACCTACATAAGTACGTCTGTGACCTCTAATTTCACTTTCATCATGCACACCACCAAGACTAATTTTAATGTAACTTTTGTTAAGCGCTTCAGCAATAGCTCTGGCTAAAGAGGTTTTACCAGTACCTGGAGGACCAACTAAAGTAAGAATAGGAACATTGTTAAATTCTGTTTGTAATTTAGCATTTTTTTCATCTTCTTTAAATAAGGACATATCAATTTGATAATTTTTGTCAATATCAATTAATTTGTCTTGGTCTGATTTTTTAGATAAGTTTTTATGGTTAATAATTAATGATAAGTATTCAATAATACGTTCTTTAACTTTTTCTAAGCCGTAGTGATTTTTATCTAAAACTTCTTTGGCACGTTTGATGTCTAAAGTTTCAACTTCTATTTTTCTTCAAGGCATTTTCTTTAATGTGCTGATGTAAGTAGCAGCAACATTAGCATCTGGTGTTCCTGACATCATACCTTTAAGTCTTTCCTTTTCTGTAGAGATAATTTTTAAAACACTTTCAGGATAAATTTGTTTTAATTTAGGATCATGAATAAGTTTTGAAAATGAGTCATCTTCTTCATCACCTTCTGAATCATTTTCTGAAAGACTTTCTTTAATAGCTTTAAGTTTTTCTCTTAAAAGAAATTCTTTTTGTTGTTTTTCTAAATTACTTTTAACTTTTTGGTTTATTTCATTTTCTAATAAAGCTTCAGCAATAGGATCTTTAGAATCTTTTTCACTCTTAGGTTTTTTAGTTAATAAGTTTTTTTTGTTTTCTTGAACTTTTTTAGTTTTTTCATTTTCTTCGTTGTATTCTTTTGCAACTTCTTTAATTTCATTAGCTCTAAAAATTGCTTCTGTTTCAAAGTATTTTACAGCTGCTTTTGAATTTGTGTTAATGCTTTTTGTTTTAGTATAGTTTTCGTCAAAAAATTTAAGTGAATTAATGATCCCTTTTTCTTTTGGAGTTCAACCAAAATTACGATATGAATAAAGTAATAATGACAAGTAATCAATATGGTCATTAACTGAATCTAAGTTAAAGATATCCATCTTATAAAGGCTATTTAAAGCAAAATAAATTAAAAGGGCTTCAACAATATTTTCGCCAGATTCATCATAATCTTCATCTCAACGAGGCGTGTTAAACATGCTTTCTTCCATTCATTTACTTTGTGCTCCTTCTGATTCTTTTAAGCTTAAATTATGATCTAAAATTTTGCTTCATAATCCTTTTTCAACTAAGTTTGTAATAGTTTTATCTAATACATTTAATTTTTTAACTGAATCTTTACTTAATTTATTTTCATAAGAACTTTTTGAAGCAACTGCAAATTCAACATTATATGAATTTTCAGGTGTTTTTTCACTATTATCTTCTAAATGAGTAATTTTATAACGTCCAATTCCTTTTACTTTAAGTTTATATCTATCGATTTCTTTTGAATCTTGTTTGTATTCAATATTTAAATCAAGCACTCTAACATAAGAACAATATTTGTTTACATTGTTACTTTTTGTAACTATTTTTTTTCTGCTTTCATCGCTGCCATCATATTTGTTTTTTGCGTAGTCAAAATAACCAATAAATAAATATTTAGCTTGGTCATTTTTTTCTTTCTTTTCATCATGACTAATTGATTCAAAATAATTAAACAAGTGATTTAAATATTGATCACTAAAACTTTGGTTTTTTGTTTTTATTTCTAAAATTTTTGTTTCATGATTATAAACTGCGTCAGATAAATATTCTTTATCTAAGACGTCCAACAGTAAGATTTTTTCTTTCATATGGTCTCCTTCTAAAATACATAATTTATATTTTAGCACTCTTATGCCTAAAGTGCCAAATATTTTTATATTTTCTTAATCTATTCTTAACATTTATTTAATAAATAAATAAAATTAGGAATTATTTGTGATTTTTGGCTCTATTTAGCCTTTATAACTAGGTTAACAATTTTATTTGGCACAAAAATTTCTTTAATAACAACTTGATTTTCTAATCATTTAGCAGCTTCATTTTTAGCTAAATTTAAAACATAATCTTTGTTATTGTTTGCTAAGTCAACTGTAATTTGAACTCTCATTTTGCCATTAATTGTTACTCCATAAGTGATTTCATCATGTTCTAAAGCACTATTATCAACACTAAAGTCCTTAAGGTTTTTAAGCTTAAAGAATTGCTCACTTAATTCTCAAGCTAAATGTGGAATAAATGGTTCCAAAATGTTTAATAAAACATAGTACATTTCTTTAATTAATAATGAGTTAGTAATCTTATCATAAGCATTGAAAGTTTCCATAGCTCAAGCAATTATAGTATTAAAAGCATAATTATTACGTCTATCACTAAACAAGTTTTCTTGCTTAATTAAACCTTGATAAAGTTTTCTTCTAGCATTCTTTTCTTCATTGTTTAATTTTAGATTTTTAATATCAGCTAACTTGTCTTTAGCCTTAACTTCTTTGCTTCTGGCTATTAATCTTTTAATAAATTTATGACAACCTTCAATACCTGAGGAAATTCATTCAAGTTCTTTTTCAGGCGGTGCTGCAAAAAGAACAAAAAGCCTCATAGTATCAGCATCATATTTTTTAATCATTTCATCTGGGCTAACAACATTGCCTTTTGATTTAGACATCTTAAGTCCATCTTTAAGAATCATTCCTTGAGTTAATAAATTATCAAAAGGTTCTCTAAAGTCAACCATTTTTAAGTCTGCTAAAACTTTAGTAAAGAAACGTGCATACAGTAAGTGCAAAATAGCATGTTCAACTCCACCGATGTATTCATCAACATTATTTCAGTATTTTAATTGAACTTTATCAAAAATATGGCTTTCTCTTTTAGCTGGTGGTGTCGTATATCTTAAAAAGTATCAGCTTGATTCAAAGAAGGTATCAAAAGTATCAGTTTCTCTGGTTGCTTTTGAGTGACATTTAGGGCACTTAGTTTCCAATCATTTATTATTTGTTTTAAGTGGGTTGCCACTAGTAGTAAAGTTGACTTTGTAAGGCAAAGTAACTGGTAAGTTTTCTTCTTTTTCAGGAACTAAACCACACTTAGCACAATTGATCATAGGAATAGGAGCGCCTCAATATCTTTGGCGACTAATTCCTCAATCTTGTAAGTTGATTTTACTCGAGTTTTCAAGCTTACTAAAATCAGCTTTGTTATTTGCATAATTAGTAATTTCTAAGTTATTAAATTTGGCAAATTCTTCATGACTTTTAACTTTAGTAGTATCGATAATTTGACTTCTATTTTTTTCTCCGAGAGAACAAAAATCACTAACATAAATTTTGTATTTTTGATTATTTACTGGATTAATTGCTTGAAGTGGTAAAGCTAAACATAATTTTTGCGAAAAATCTTTTGCTGTAGCTTTTGCTTTAATTAAATTAAGTTTTTCAATTTCTTTATCACTTAATTTATTTTCTTCAATTAATTGTTTAACAAGGCCATGATTTGCACCAATAGTTATAAAATTCATGGTTACTAAAATATCTTTGTTTTTTGTAAAAATATCAATATTACTAAAGTGATTTTGATTATTTTCAACTATGTCAAATTTTGCCAAAAAGCCTGTTTCATAGCCAATTCAGTTTTTTTGCATCATTAAAACTTTGTCAGGTCAATGATTTTTTAATAACTCTAAATCATTTTGTAATTCTTCTGCGTAGTTTCTAATTTTTAAGTAATATTGTTCCATTTCTTTTTGAACAACAATATTGCCACAACGTCAACATTTTCCATCTTCAACTTGTTCATTTGCTAAAACTGTTTGATCATTTTCGCATCAATTTAATAATGCTTTTTTACGATATACTAAACCTTTTTTTCACATCTTAATGAAAATCAATTGTTCTCATTTTGTATAAATTTCATCAGCTGTAATACATTCATAATTTCAAGCAAAAGAGATTCCCAAATCCTTTAATTTAGGGTTCATATTTTTAATGTTTTTGTATGTTCAATCTTTAGGGTGAATGCCATTTTTAATAGCTGCATTTTCAGCTGGTAAACCAAAAGCATCTCAACCAAAAGGATGTAAAACATTAAAACCTTTACGACGATAATAACGTGCTAAAACATCGCCGATTGCATAGTTTCTAACATGCCCCATATGAATATTTCCACTTGGATAAGGGAACATACTTAAGATATATTTCTTTGGTTTCTTGGTTGTTTTTTGTGGTTCAAAATATTGAGTTTCAGCTCAATAATTTTGTCATTTTTTTTCGACTTTATGTTGATTATATGTTGACATTTTGACTCCTAAAATGATGTCTAAATTATTTGTGATTATCATTTAATTTTGCATAACAAGCTTGAATAAAAATATTTTTAAAATGTTTCTTTTTATTGATTTCAAGTAAATCGGATTTTCAAAGTTTAGCTTTAATATTGAAGGCTAAAAAGATAAAAATTATTAAAGCATTTAAACTTAAGTTAATAATATTGAAGAGAGCAAAAATAGTGCTAGTGCCTAAATAATAGTTGTTTATATTAAGAAATAGACCTTTGAATTTGTCTCAATTTTTTATCATTGAATTAATTGTGGCAGGTTCATTTTTTAATGCGCCAAAAGCTTTAAAGTATCAAGGTGTTGTAATAAAAACGTTAACAAAAGTTAAAAACAAAGTTGTAATGACTGCTGCTAAAGATAATTCAGCAATATAAAAATCTATTTGTTTTCGAATTGAATTTTGATAAATTGTTTTATTAAAAAACTTATATAGAAAATAAAAAGTTAATACAAAAGTTAGCTGAGTTAAAGCTAAAAGGAAATGTCCTAATAAACCAATAGGATCATAACCATTGCTGCCATAGGCAGGGCCTATTATTGCTAAAAGTAATATTAAGACTAAAGCTGATCAAGGTGCAATTGCTAAAGCGCTAAAAGTAACGACAACAAGTGCAAAATCAAACTTTAAAAAAGGCGCTAAAGGAAATTTCATAAAGCTTGAGCAAGTAATATTAACTACAATTGCTAAAGCTAATAAAACTCCAGTTTGGACTATTTTTTTAACAATACTTTTGCCATAATAAATACTTTTATTTTGTTCCAAAAATACGGTCTCCTGCGTCGCCTAAACCAGGTTCAATATATTTTTGATTATTGAGTTTTTTGTCTAGAGCAGCTAAATAAATTTCAAAGTCTTTACCAAAAGCCTTTTCAACAGCTGCTACTCCTTCTTTAACTCCAACTAAGCACACTAAATGAATATTAGTAAAGCCATCTGATTTTAATTTTTTAATAGCATCAACTGCGCTACCGCCAGTAGCGAGCATTGGATCAACTACAAAAATGTAGCTATCTTTTTTAACTTCTGGCATTTTGTAAAAATACTCATGAGCTTGATGAGTTACTTCATCGCGGTAAAGTCCAATGTGACCAACTCTAGCTTCAGGCATTAATTCAAGTAAGCCATCTAACATTCCAAGCCCAGCTCTTAAAATTGGCACAATAACTATTTCTTTATCAAAAGTAGCTCCTACATATTCTTGATTTAAAGGTGTCACTATTTTTCTAGCTTTAGTTTTATAGTTTCTTAAAACTTCATACACCATTAATGATGCGATTTCATTTAAGTTTCTTCTAAAACGAGAGTGATTTGCTTCTTTATCTCTCATATTAGTCAGCTTAATTGAAATAAGCGGATGATCTAAAACTTTTAACATCTTTTTTCCTCCTTATATATTAATCTAAATAATAATACTATATATTAAATATATTGTTTGCATTTTTAATAAATAATATATATATTAAAAAAATAAGTTGGCTGAAAAATGTCAAAAATTAAAAAAGAAAAATGCAAACTAGTTGCATTAATCTAATAAATTAATATTTATTAAAAGGATTTTCTAAGTTACTGACTTTTTTGCCTTTTTTTAATAATTTTAACTGTTTTTTCACTGGTTTTTGAATATTTTTTTCTTTGTTGACTATTTCAGAAATCTCTTTTAAAATTAATCTTATAAAATTTATCAAGTTGAGGATCTAAACTAAAGATTAAATAAAAGTAGATTAAATCTGTTAAAAAGTAAACACTAATTTTTGAGCCTAATGAAGCAGAAGTTTTAATATGTTGAGCTAAATTTGTTAAATTTAAAATACTTAAAGCATTTAATTTTGCAATACTTGTGCTTTGAGTTCATAAAGCATAATTAATGTTCTTGTTTTCCAAAATTTCACAAACTTTTATAACTTCAGCTGTTTCAAAAGAATGCGAAATAACTGTAACAAAAGCATCTGGACCCATTAAACATAAACTATGCTAAAAAAATCATGCATATTGTTAATAGGTAATGAAGTAAGTCTAATTTTGTTTAAATTTTCACGTAAATAACTAGCTACTTTTTCACTTTGCCCAATACCAAAAAATATGTGATTTTTAGTTTCGCTTAATAAATTGCAATACTCTTTAATTTCTTCTTTGTTAATTGACTCATAAGTTTTTTGTAAGCTTGAAGTATAGTAAGCAAAGATGTTTTTACTTATTTCATGCATACTTAAATTTGAACCAACATTGATTCTTGTTCAATCATCTGTTACTATTGTTCTTTTAGTAATATAGATTTGTAAAGATTTAAAACTAATAAAACTAGCTGCTCGAGCAAAACGAGTTAATGTTAGCTATGAAACAAACATTTTTTCAGTTAATTCAATTGAAGAGTTGTTTAAAAAAATTTCTGTTTTATTGTCGATATAATCGACTATGAATCTATACGTTTCATTTTCAGGTAAAAATTTTAAATATTTTTTTGAAGTAACTTTGACTTTAAATTTTTCATATCTTAATTTTAACAAATTAAAAAAATAAAATTCGAAGTTTAAAAATTATAGTGAACCCCAAATTCCGGACTTTTTGGAAAAGGGTTCATACCAAATAAGATAATGTTTTTTGAGCATTATTTTATTTTGTTCTACAATATTATTAGGTGTTGATGTAAAGTCAATTCTTTTCTATTGAGAAAGTGAGAGACTTTACACAAAATTATAAATAGTCTTAATAATATTTTTTAATTTAAATAATAAATGAACCCCTTTTTGAAAAAGAAATTTGGGGTTCAGCATAATATTAAATTTCGTTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTTCAGTAAAGATTTTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGATTTATTTTTCAAAATGTATCTTTCGGTGATTGCATATTCTGATTTTCAGTACATTTTATTTCATATGTAGTATGAAGCATCAAAGTTTCCTAATGGGTTATCAAGAGTAAATTTAAAAATGTAATTTACTCATGAAATAGGAGCTCCATTATGTTGTAATATGAAAGCTGAATTATGTTCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCACGTTTTGTTATATAGTGAGGACCATAGAAATTACTTGGTAATACTCCAATAGGATATTCTTCTCCTGTTTTTTTGTTATAAACATTAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTGTTGTATTTAATAAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCCTTGAAAATCATCTTTAAACATTATTTTAGAATCTGACATTTTTTTGATATTTGAAAGCATTTTTTCAGTTATTGTTTTCTCATATTCTATTAATTTGGCTAAAGTATATAGATCATCATGTCCACTTCAAATATCATTTTGTATTGCTTTTAAATAATCGCCATTATTTTTATTAGGATATGCTTCTTTTATATATTTAACTGTTGGTATACTATAATCTAGTGGTAGATTATTATCTTCTGGAGGTTCAATAATTGTATGTCAAGGCCTTCAATATTGATTTTCAATTTGCTTACCTTGTTCGATTTCACTAGTACTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTGCCTAGAACTTTAGGTTTAGTTCAAATTTTTGATTTTTCATCTCAATTTGCATTATCTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTATGAAATATTCTCTTAAATCTTTAACAGCTAATAAATAATCATTTTCTAAATAATTTTTAATTAAATTAGTGTTTGTAAAATCAACGCCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACACCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAACAGCACTAAAGTTTTCTGTAGAATTAACATTTTTATAAGTTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCCACATTTGCTATTGCTGCTAATTTTGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAAGAAAATTATTGATTAAATTAATTTTTTCAAATAGTCCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTGTTTTCAATTGAAGATATTTCTTTAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAATTTGTTTCATATTTTTTAAGATTTGAAATTATTTCAAAGAATGCTGAAATATTATTAATTTTGTTGTCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTAGTTTGTATATCAATTATTGATTTATAAGAAGTTTCAAATAAATTAAATCCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATGTTAACAAAGGCTTTTTTAAATCAATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATAATCATTAATTAATTTGTTTCTGCCTTCAATATACTTGTCTCATTTGAGAGAATTTAATTCAATTTTAGATTTTTGTACAAATTCATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAACTGTTGTTTCAATTGAATCAACATTTGAATTATAAGCATTTAAAAATTCTTTAATAACAGATTGAATAGAAGTTGTCAAATTATTTTCGTCATTTAAAATAGCTTGAGTTTTGTTAATAAATTCGTCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAACAAATGAATTAAATTCATTAAGACCATTAATAATAGATGTTAATGATTTGCTGCTTAAGTCTAGAATTTGAGATTTGTTAAATAATTTTGTATTTTCAATTGTTTTGACAAAAGTATTAACATAACGATTTATATATTCTTTTGTATTTCCATATGATTGTTCCAATGTTTTACATGCCTTAATAATATCTTCTATAATAACGCTTCTATTTACTGAAACACCGTTTTTTTCTAATATAACGTCATAATTATTAGAGTCAAATCTTGACAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTAGATATGTTTGACATGAATTTTTTAATTAAACCATCTTTATTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCCTTATCAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTGTTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAATGTTTTGATAATCTTTAATTTTGAGTTCTAAAGAATCTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCGGTTTTTAAAGTTAATAAATCATTACTACGAACAATAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTATCATCTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTGTGTTTAGCAGCTCTTATATAACTTTTGTTACCAAAGTGGCGGCCAACATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAATCAATTTGAGTTTGGCACAAACTTAGTTCATTTCAAAGACTAGAAAGTCATTCTTGATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAAGTATTTTATGCTGTTTTCAATTCACAATTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCGCCAACAACCCCAGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAACATAAAAAAATTAAATTTTCGGGACATAAGGAAAATTATGTCTTAAAAATAACCATAAAAAGTACCAAAAAAAGGCAAAAATTATTTTCTAAAAATACTATAACTTATAAAAGTAAGACATACCATTAAAGATTTTTAAAATTTTGAAAAAAATTTTATTCTTCATTTTCAAAATCAATAATTTTAAATTCATTTAATATGCTAGATCAATTTCTAATTCTTGAATTACTTCATTTTTTTATGATTTGTTGAGATGCTAGAAAAAGAATTTTTAAAAGACTATCATCAGATGGGAAAACCTTTTTTGTTTTTGCATATTTTCTAAATACACTATTTAAATTTTCGATTGGATTAGTGGTATAAATGAGCGTTCTTATTTCAAGGGAGTAATCAAACATTGTAGAAATTTCAGATCAGTTTTGTCTTCATATTGAAATAGATGTAGGATATTTTTTGCCTCATTTATCTTCAAAAATATCCAAATTATCCATTGCATTTTTTGCATCATTAGATTGATATACAGATTTTAAATCTTTGCAAAATTCTTTCATATCATTTAACAGAATTTCTAATCATATGTACAATGCAACGTTGTTGCTTACTATTAGGAAAAACAACTTCTATAGATTGTTGTATACCTTTTAAATTATCTGAGCAAATAATATCAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGTCGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGCCCATCACTATTAATACCCATAATAACATATGTAGCTTTCTTAGTTACAACATTATTATCTACAACATGAAAATGTACTGCATCAATATAAATTATTGAATAATATGTTTCAAGTTTTCTTTCTTGTCATAATCTTGCGTCTTCGACAATTTCGTTAGTTAATTGACTTATTTGTGCTGGAGAATAATTAACACCATATTTGCTAAACATAAAATCATTTACATCTCTTGTTGACATTCCTCTAGAATATAAAGCAAATATCATATCAACTAATTCAGAAATATCACGTTCACACTTCTTGATGATTATAGGTTCAATTTTTGCTTCACGATCTCTTGGAATGTCAATTTCCATTTGGCCATATTGACTATTAACATTTTTCTTAAAAAAATCATTTCTATAATTTTTTTTGAACTTTATTATCATTTTTTCGTAACCTATAAAATTATCAAATTCTTTGTTCATCATTTTTTGGATTGTATTTTGAAATACTTCTTTCATAGCCATATTAAGATCACCAGTAGTAACTTGGTTTTAATTTTCTAAATTTTTTTGTTATTTCATCAATAGCAGGATTTATTAACCTTCTTTTCATAAATAAAAAAATCCTTTCTTAATTGATAAAATATTATCAGGAAATGATTTTAAGACTTTACACAAAATTTTTTATAGTTCCCAATTTTAAAAAACAGGCAAGACCTGTTTTTTAAATTAATCTGTTTTATAGTTGTTTTGGTAAAATACCAGTTTATTCAAGTTTTGTTCCATCAGCTAAATCTATGGCTACAAAAATGTCTTTAATTTTACTTTTGCCAATTGTATATCTAGTTATTTCTTGTAAAACATTAACAGCTTCTTGACATCTTCTTTTAGCATCTTCAGCATAGTTAAAACCAGCATTTAAAAAGTTGTCATCTTGGATTTTGTTAATAAAATTAGTAAAATCATTTTTTGCTTTTTCTCATATTTCTGCTAATTTTTTATGACCGCTTTTTTTAAGTTCTTCTTCAATAACTAGAACATCTTTTTCAAAGTTTTCTTTAATTTCTTTTCTTAAAGTTAATATGACATTGTATAACTCATAGATCATATTTCCAATTGCATGTGAGTGATTATGACCACTGTTATTACTTGAACTACTATAATTTGAAACTAATTCTTGATTGCCTTTTTCTTTTCAAGCTTTTGGATGTAAATTAGCAATAATATTTGAAATTGATTTATAGTCCGTACTCAAAACAGTTTCTCAGTCTTCATTTAAAATTTGTTTATTTTTTTCAAAATATTTATGCTTAAGAATATAGAATCTACTTCTTTGTTGTTGATTTTCATCGTCTTCTGTTAAACCATCTAATAATCTTGAATCATTGAATATTTGAGCGTTAACATCTAAAGCTAAATAAAGCATTTTATCGAAGAATTGGTAAAATTTGTCTTTTCCACTTTGACTTGTAAAAAAGCTCAATTTGTCTTTAAAATTAACTGCTGATTCAACTTCTTTCATTAAAGTATTTAATTCAACATTATCACTATTTATTATTCCGGTTTGACTGCTGCCAATTATCATATCCATCATGTCACATATTTGAGCTATGTTTTCTTGATTTTTCGATAATGTCATATCTAATGATTTGTCATACAAAACATTATTGTCATATTCAAAAGACAAATTAATTCTAGCCTTATTGATTAAGTCTAAATATTTTTCGATTTTATCGACAACTGGATTATTATGAATATTTTTTTTTGATCATTTATTTGTTGAATTTAATGTGCAACCCGCTGAAATTATTAAGTTTGGTGCTATTATTGTAGGCAACAAATAAAGTAATATTTTTTTTCTTTTGATGAATGATTTATTCATAAAAACTCATCTCTACACTAAAAATTAGTATTTTTTATACATTTATTATATATTAATCACTCAAAAAAACGCAAAAAAGAAATTTTTGCCTTTGAAGGTGTTAAAATATAGTTGCAGCCTTGAATAAGCTGTCTACACAAGCACAGGTTCGAATTTTATAAAAAAATTAGAAATGAGCTGTGTATTATAATTCGATTTAATTTTTCTTTTTTCCCTTAAAGAAAAATTGAAACAAGCGTGCGGAGTATGCTATTTAATTTACAAAATTATTTATCATTTTTTTAATACCCTTTCTATTCTATTTTTTCATCACATTTACTTACCTTTATTTTTTCTGTCTATTTTTTTATGTAAAGGTTAAAAATTTAAAACACGCTTCTCTGCACTTTAGTGTTTTAAACAAAATATTATATTAATAATAATGCAGGTTAACGCCTGTTTTTTTATTATATAATTTAACAAATTAATAAATAAGGAGAGCTATGAATGCTTTAGATATTAAAAAGAAAATTATTGAATTAAGTAATAAATTAAACCAATGAAATAAAGAATATTATCAAGATAATAATCCTAGTGTTAGCGATTTTGAATATGATAAAGCTTTACAAGAATTAGAACAATTAGAAAAAAAATATCCTCAATTTATTTATGTTAATAGTCCTACTCAAAAATTAGGATCTTTTGCTGATAATAAATTTAAAAAAGTAGTTCATCAAAAACCAATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGAAATAAAATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAGTTATTATTTTCATAATATTTAAAATAATTTTTGAGTATTTAACTAAAATACTTTGAGTTTTTATATAATTTAATCAATTATTTAGGAGTTATTATGGATATAAGAAAATATGTTGTTATTGGTAACTGAAAAATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGAGCTATTGGAACAGGTAAAGTTGCAACTCCTGAAATTGCTCAAGATGTTTGCCAATACATTCACTCAATTACAAGTAAAGAATTAGTTGTTCAATATGGCGGTAGTGTAAATCCTAAAAATATTAGCGATTTAGCAGCACAAAAAGACATCAACGGATTCTTAGTTGGTGGTGCAAGTTTAAATGTTGATTCATTTATTAAACTTATTACTTTAGGCAAATAATTTAGATTAAAAACAGCCCTCTTTGCAAGGACTTTTTTAATTGCGATTTTGTATTAAAACAACTGTTTTATAGTGTTATTAGTGAAATTATTAAATTTTATTTTTGATTAATTTCGCTGATAATTCATCAACAATAATTGTTACATCTTTATGATTTTGTAAAAAGCTGGCTGGACAATTTGAATTTACTGGTTTAAGTAAAGTATCATAAATTGCTTGAGCTTTTTTAGGCCCGGTTGCTAATAACATAATTTTTTTACTTTTTAAAATAGTTCCTATTCCCATAGTTACTGCTTTACGAGGAACATCTTTTTCACTAGCAAAAAATCTTTTGTTTGCATTAATTGTGCTTTCAGTTAAATCAACTACTCTTGTAATATCTTGTTCTTGACTATCTGGTTCATTAAATGCAATATGACCGTTTTCGCCAATACCTAAAATAGTAAAATCAATACCTCCAAGTTTTTCAATTACTTGTTCATAGCTACCATTTTGATGTTCTTTTTCATATTTTATGACATCAGGAAAATTGATATTTTCTCTTTTAACATTAATGTGATTAAACAAATTATGATCCATAAAATAATGATAAGAATGTTCATTTTTTAAATCTAATCCAAGGTATTCATCCAAGTTAAAAGTAACCATTTTACTAAAATCAATTTTACCTTTTTGATAATCTTCAATTAATAATTTATATAAATCTAAAGGTGTTGAACCTGTGGCAAAGCAAATTCTTGAATCTGCTTTTTTAGTAATTTGATTCTTAATTAAACTTAATGCTTTTTGTGCTACTGCTAAAGCATCTTTTTCAATATATACTTTCATTTTTACTCCTTATTATTTGACTTTTTGCCCATCAATATAAACTTCTTTAATATCTAAAGTTTTTAAATCCATTAAAACTAAATCTGCTAATTTATTTGCTTTAATTTGTGCTCGATCTTTTAAATTATGAGCTTTAGCTGCATTATATGAAGTTAATTTAACAATATCACGATAATCTAAATCTAATGAAGCTAAATTTTTAAATGAATCATGTAAGCAAATTCCACTTCCAGCTATAGTTGTTGTGCCCTCTAAATAAATTGTTAAACCATGTTTTTCAATATTAATTCCACCTGAAATGCTTTTACCATCAGGTCCATAAGCAGGTCGAATTGCATCGCTAACACACATTAAGTGGTTTATATCTTTATGATTAAGCGTAAAAACTATACTTTCTTGACAAATGTGATGAAAATCAACAATTAATTCACTATAAACATCATGTCTTTCTAATGAAGCTTCTAAGATTCCCGGTTTTCTTGAATCAATTCCACTCATAGCATTTCATAAGTGACAAGTATTAGTTGAACCCAAATCATAGTATTTCATAGCTTGATCATAGCTTGCAGCTGAGTGACCAATTGAAGGCATAATGTCATTTTGAACCATAAAAGGAACTAATTTTTCTTCAACCATTAAAGGATCATAACTAATTTTTTTCAATTGATGATTTGAACTATTTAGCATTTCTAAAATATCTTCTCTAGTTGCTCTTCTTAATCATTCTAATTTGTGAGCTCCTTTTTTAGCCTCTCCAATAAAAGGACCTTCAATATGAATACCAATGTTTTTAGCACCTTGACATTTTGCTAAAGCAATATTTTTTAAAGCAATCTGAATATTTTCTCAACTTGCTGTCATTGCTGTAGGCATAAAACTAGTAATTCCATTTCTAGCTAAATTTAAACTAATATCTTCAACTGCTGCACTACTATCCATTACATCCTTATTCATAAAACCATGAATATGAGTGTCAATAAAGCCTGGAACTAATAAATATTGAGCCTTATTTTTAGTTGGAGTTATTTTAGTAATTTTTTTACTAAACTCAATGTCAGCACACTCAATAATTTGGTTGTGATTAACTATTTTAACGTTTTTTATTAGCATTTTTACCTCATTTGTTTTTTAATAATTTAATTATATTACTTAACTTTTTTTGCCTAAATTAAAAGCAAAAAATGGTAAATAAATTACACTTATTTGCTTTATTTTTGAAAAACCTTTTCAATAAGGTTTCATTTTCACAAATTTTTATTTTTTCCTCTATTTATATGTACATAAAGCATTTAATTGTTTATAATATAAACATATCTTTTAAATAATTGAGGAGTTATTATTTATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGAGCTGAATTAGGCGATGTATTTATTAATGATGCTTTTGGTACAGCTCACAGAGCACATGCTTCTAATGTTGGTATTGCATCAAACATTAAAGAAAGTGCTATTGGTTACTTAATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGAAATGGACCTATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTGCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAGTTTAAAAATAATAAAAAATGCGTAAAGCTCTGTTTTATAGGCTTAAACGCATTTTTACTTTTCTAAATATTCATTCATTTCAAGAGCTAACTTACGTGCATAATCACCAATAACTAATGAAATTTTATTAGTGCTAATCATAATTCCTGTAATGAATTTTGTATCTTGCAATTTATCCATTTTTATTAATGTTTTATCTTTGATTAAAACTTTAATATTTGAAATGCTTGCATTTACTTCTTCTATGTTTTCTTTGCCACCAAGGTTGTCTACTAATTCAACAACTTTGATATTTGAAGGTAGTTTAATATTAACTCCTTCTTTTTTAATCTTTTGTTCTTTTTTATTTTTTAGGTGTCAATATAATCAACAAAAACCTAAAGTTACAATGGTTAAAAGAACAACTAAAAATTTATCTTTTTTACTCATATTTTTATTATACAAAATAAAATTTAAAAGCAAATTAATAACTTTGAACTAAATTTAATTTATTACATTTCTCTTTATCTTTTACAAAGATAATAAAATTGTTGTAAAATAAAAAATAGTTATATATGCTTAAATAAAAAAAGAGGTAGTTATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGACTTATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAAAATGGCAAAAGATAAAAAAATATTACAAAAGTGTTAAACCTGAAGAAATTTATATCTGTGGAATAGACACAGATTGTTGTGTTCAAGCAACAGCAGTAAACTTATTTGAAAAAAGGATTAGACCAATAGTTTTAAGCAATTATTGTGCTTCAAATGGCGGTCCTGAATCTCATGAAGCAGCTCTTAAAACATTATCAAGAGCAATAGGCAAAGAAAACATTATTCAACTATAAAACAGTTGTTTTAGAAAAAAGAAGGTATATATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGAAGAGGTAACAGTGACAATATCCAATTAACAAGAATTTATGGAACAAGTTGAAGCAAAAAAGAAGAATTGGATAACTATTTAGCAATATTAGCTGATAGAAAAGAAAGAGACCACAGAAAGCTTGGTAAAGAAATGAAAATTTTTGCTATGCATCCTTTATGCGGCCAAGGTTTTCCAATTTGACTTGAAGATGGAATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGAAATAGCGCTGAAAAAATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGATTAGCGCCAAAACAAATTACAATTATCCCTGTTAATTTAAATGAAAATTTAGATTACTCTAGAAATATTTTCAATAGATTATGAAATTGTAATTTCAGAGTCAAGATTGATGATCGTGATGAAAGATTAAATAAAAAAATTCGTGAGGCTCAAATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAGTAAACAAAAGAAAAAAGAAGTTAAAACATGAAGACCATTAGTCAATGTATTATTTACATTGCTCTTTTGTGTTGTTTTTCCTTTTGTTTGATGATTATTTGCAACTGTTGATTATCATAATCAAAAAATTACAAATTTAGCAATTTGTATTTCGGTAATTGTTATCTATTCACTTTTAATAATAGGATTAAATATTTTGCTTCATTATTTTAAAGTTTTAAATATTAGAAGTTATAACTTTAATATTCCAATTCTTTCAGTTTTCTTGTGAATTATTTTAACTTCATACATATCTAAATTTAATATTTATGCTCGTGTTGGAGTAGCTATTGCAGTAGTAGTTGTAATTACTTTAATAGTTAACTTTACAACAGGTAAAATTGAAGATAATATGGAGAAAAAAGCTAAACAATTAGAAGAAGAAAAACTTAAAAAAGAAATTGAGGATTTGAACAAATAATACTATAAAACAGTACTATAGGTAAATTCTAATAAAAAAATTATCAATAAATAAAACTAATTTATAATATTATTAATTAGTAGAGGAGTTTAGTAATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAATTATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAAAAGTGGGATTTCTTTTTATTTAATTAAAAAGCAAAATAAAAATAGCTTGTTAGGCTATTTACTATTTTTTGATTGTGTTTGATTATTTTCCTGATTATTATCTTTATTTTGTTCTTTTTCACCTTCAAAAGTTAAATCACTTTCTAAAGGCTTGTTGACAATTTCTTCTTTGACTGATATTTCAAGTCCAAAAGGAACTAATAATTGTAAAGCTAAGTATATTAAATAAAAGTCATCCATTAATTTATTTGTTAAGGTTTCAATAATATCTTCTTTATTAATGGTTTTGGCTTCAAAGATTAAATCAATTAATTCAAAAGGAATGTCAAATCAAATAAATGCTTCTGTATTTCTAATTAAGAATGATAAATTATTTAATAAATATTTTTTATTCGCTTTAGTGTTAGGAACACTAATTTGGCTAATAGTCAATTCATTGAAAATTTCATTTAAGAATTCTAAATTATTTTTTTGATCTAATCAAGCTTTTAAAACAGCATGATTATTTGATTTAAGTAAAAGTTTTTCGTAAGCTTTTGGTAATTCAACGCTTGTGTAATCACTTGGTGTATAGAAGTTATCTTTATCAAAGAATGACACATTTACTTCGGGATTTAGTTCTCTAAGTTTAACGGCAATTCCGAAGCGTTTAACACTTAGTTTTTGTTCGATTAATTGTCCATATGTTCTGCTTTTAGCTTTAATTAATTGTTTCTTAAGTTTATGATTAATTGAGGCTAAATCATGTCAAATGTTGCCTTCGTAGTAAACATTTTGCATCTTTAAATATTGGTTATCAGTTAAAGCTTCATATTGTTCATATTTTTTATGGATTGGAGCAAAACCAAATTCAAAATTTTTGAAATTATGTGTGTTTTTATAAAAATTGATTAAGTCATAATTTTTGTGAATTTTGTTATTTTCATCAACGATATTTTCTTTGTAACCAAGAGTAAAGAAGTTAAATAATTTTTTGATTAATTTATATTTATCAAATTTGGTAATAACATCTTTCAGCACATATTTTTTAGCTTTTTGTCCTATTAAAAGAACAATTAAGCCAGGGATAGTTAAAGTTAATGAAATACCAAATAGCATGCCAAAGAATCATTCACTATTGTTTTCGAAAAATGTTTCTTTTTTGCGAAAGTAAAGAGCAAAAAGGGCAATGGTTGTTATTAATAATAAAAGACCTAATAAAAATATTCCAACGCCACTTCATAAACCGCCTTTAGCTCAATTAATCCTTTTGCTTTTCTTTTTATCAGCATAATAATTATTAATTAATTGAGTAGTTAATTTGTTTATTGTTGGACGTTTAGAACGAATAAAAGCTTCTTTTGAAATTAACTTATTTGATACTGACATTTGACCTCATTTCTATACGTATTATTATAATATTAATTATTTAATTTATTTATTATTTTGTTGTTTTTAGTATAAAAAACTTTTGCCTAAGCAAAAGTCAAAAATGGTAATTTTATTAATGTATATTTAGTACCCTTCTTTTGAAGATTTACCTTCAACAATGGCAACTGAACGGCTTGTTCCGAAACGGTTTGCGCCAGCTTGAGCCATGTTGATCATGTCATCTTTGTTGCTGATTCCACCAGCAGCTTTGATTAATAATTTGTCTCCACAAACACTTTTCATGATTTTAACATCTTCAAGTGTTGCGCCTCTTGTTGAGAAACCTGTTGAAGTTTTAATGAATTCAGCATCAGAAGCCATAACGATTTCAGTAGCTTTTCTAATTTCAGCTTCTGTTAATAAAGCTGTTTCAATAATAACTTTTAAAACATGTTTGCCGCATGCTTTTTTAACCGCTTTAATGTCTTCTAAAACATAGTCATAGTCACCCATTTTCATTTTTCCAATTTGAATAACCATGTCAATTTCATCAGCACCAGCTTTAATAGCTTCTTTAGCTTCAAATGCCTTAGCACTTGTAAGCATAGCACCTAAAGGGAAACCAATAACTGATGTAATTCCAATGTTTGTTTTAGTTAATTTTTCTTTGCAGTATGCAACTCATGATGAATTAACACAAACAGTTTTGAAGTCATATTTCTTGGCTTCATCTAATAAATTATCAATTTCTTTATTTGTCGCATCAGCTTTTAAATAAGTGTGATCAATCATTTTGTTGTAATTAATTTGCATAATTTTTTCTCCTAATTTTTTATTATTTCAACTTAGCAATTATAATCTTATTTTCAACTTTTGAATTGCCAATTTTGTAACCTGTTTCTAATTCTTTAACTAAATTCTTGTCAATCGGATTTGAAGAATAAAGAGTAAATAGTTTTTGCCCTTTAGTTACTTTATGATTTGTTTTAGCGTGAAGAGTAATTCCTGCTTCAAAGTCAATACTATCTTCTTTTTTAGCACGGCCGGCGCCAAGTTTCATAGCTATAATACCGAAAACTAATGAGTCAAAAATATCTAAGTAACCATCTTTAGCAGCAACAATATCTAAAGAATGTTTTGGACTTCAAAATTTAGGATCTTGTAATGCTTTAACATCACCACCTTGAAGTTTGACAAATTCATAGAATTTTTCAAGGGCTTTGCCATTTTTAATAACTTCTTCAACTTTTTTAAGCCCTTCAGCATGAGACTTAACTAATTTGGCTTGTTCTAAAATAGTTGCGCATGAAGAATAAACTAATTCATTGAAATCTTCTGGACCTTTGCCTTGAAGAGTTCAAATAGCTTCTAAAACTTCATTTTTGTTTCCAATTTCACAACCAATTGGACGGTTCATGTTGGTAATTTCTGCACGAACATCAACGTTTAATTCTTTACCAATTGAAATCATAGTTTTAGCTAATTCTTTAGCTTGGTCTTCATTTTTCATGAAGGCGCCTTTACCGCATTTAACATCTAATAAAATAGCATTTGAACCTGTAGCTAATTTTTTAGACATAATACTTGAAGCAATAAGAGGAATTGAGTTAACACAAGCAGTAACATCTCTTAAAGCATAAAGTTTTTTATCAGCTGGTACTAATTCTTTTGATTGACCAATAACTGAAATATTATGTTTTTTAACTTGTTCAATAAATTGACGTTCAGTTAATTCAACATGAAATCCTGGAATAGATTCAAGTTTATCAATTGTTCCACCAGTGTGGCCTAAACCTCTACCACTCATTTTCGCTACTGGAGCACCTAAAGCGGCACAAATAGGAGCAACTGCTAAAGTAGTTTTATCTCCAACTCCACCTGTTGAGTGTTTATCAACTTTAATCCCTGGAATTTCACTTAAATCAATAACTTCACCTGAGTGCATCATGGTTTTAGTAAAAGTTGCTAATTCTTTTTTTGCCATTCCTTGGAACATAACTGCCATTATAAAAGCAGCCATTTGGTAATCAGGTGTATTACCTGATACATAAGAAGTAATTAAGAATTCAATTTCTTGAGTTGTTAATTCTTTACCTTCTCTTTTCTTTTCGATTAAATCAACGACACGCATTATTTAACCTCCCTTATTTTGCAACACCTAAAGCAATTTCCATCATGTTTTTGAAAGCTGTTTGTCTTTCTTCGGGTGTTGTTTCGTCATTTGTAATTAAGTTGTCACTAACTGTTAAAAGACATGCTGCATTTTTACCTAATTTTTCAGCATTAGTAAATAAAGCATATGATTCCATTTCAACACAAAGCGCATGTGTTTCATCGATTCTTTGTTGAAGAGGAACTGATGAATAGAATACATCTGAAGAGTGTACACGGCCTAAAGTTAATTTAATGTTCAAATTTTTAGCCATTTTTTCAATTTCTTTATTTAATTTAGCACTAGGTAAAGCTACATGGCTTTTGTCTCCTAAAACTAAACGACGATAACTAGCACCATCTGCATAAGCTTCAGTTGCTAAAATCGTTTCATATAGTTTGATTTCTTTTTTGTATGAACCAGTTGTACCGATTCTAATAATGTTATCTACATTGTAGAATTTGAATAATTCATATGAATAGATCCCAATTGATGGACAACCCATTCCACTTCCAGCGATTGTTACTGGACGACCTTTATATGTACCTGTATACATGTACATGTTTCTGACAGTATTAACAAGTCTATATCCTTTGTCTAAAAATGTTTCAGCAATAAATTTAGCTCTTAAAGGATCTCCTGGCATTAAAACTGTCTTAGCAATATCACCTTCTTTGGCATTAATGTGTGGTGTCATAAAATCTCCTTAAATTAATGTTAAATAAGTACTTTCAAGTTTGGTTTATATTTAATTTCAAGTACATTATTGGACATCAACTAATAGAAATCTGATAAAGTGCGATTTAAGTAGTATTAGCGATTTAGCTTAATATTTTCTATATTAAACATCCGGTTATAATTTTAACATATATTAAATTTAAAATATATAAATATCACTATTAGAGCTATTTATTTGATTTTTTTAAGCTAAAGGTGTCATTTTTTCATATTTTAGTTGCCTAAAAAATACAAAAATAAAAATATTTTCATATTTGTTAATTATCAGGCTAAATAATATTTTTATTTAGAAACTACTTTTTATATAATTAGTTAGATATTTAGATACTTTTTTTGTCGAGGTGAATTATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGAGAAACTAGACCTAATGTTTTAGGATACATTCAACGTGGTGGTAATCCTTCAGCTCAAGAAAGAATTCTAGCTTCACTTTTTGGAATTAAAGCTGTTGAATGTTTACTTGAAGGTAAAAGCGGTGTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCCCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAGATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAATCAATTATAATGTCATTTATTTGGCATTATTTTAATTTTTTAAGCAAATTTTTCATTATTTTTTTTAGAAAAATATTTAAGCTTATTTTTTGAATAATAAACCTATAAAACAATGTTTTTGGTAAATTACACTATTTGCTTTAAACTAAGATTTTTAAAAAAATGTTTTTGTTTTTTTAATAATAAATTATTATTATATAGGCGCATTTAGAGAAAGGGGCAAATTATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTAGATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGAAGTAGTGATGTTTTTTATGTATTTAAAGAAGCAGATAAAGAATTCCTAAATAAAATTTATTATCGTTATGAAACTTTTGAACAATTCGAATTATATGAAGATCAAAAAGACATTAAAAAAGTAGCCAAAATTGAAGTTTATTTTGAAAACACTCCAAATGTTGGCGATTTAATTAAAGAACTTGAAAAACTTGATTGCCAAGTAATTGTGACACATATGAATCTTGAAATTATTTCTAAAGGAGCTTCAAAAGGAAATGCTATTCTTTGACTTCTAAAACATGTATTAAAAGGATATAACCCAGAAGAAGTTATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAGTCAGTGTACTAGTTTTTTTAATATTTTGAATATTTTTAATAAAAAATAAGCAAATTTGTCAAAAAACCTGCAATATTACATACTTTTTTTACAAAATTAATCAAAAATTAATCAAAATTGTTAAAAAAGTCTGTCATTATGACAAATTAATTGTATAGTTTCTTATATAAATATAAGGAGTTGACTATGACTATTGAAAGAGATAAATATTTAAATGGTCTTTTAGAATGAAAAGATAAAGACATTGTAAAAATAGTAACTGGTCCTCGAAGAAGTGGCAAAACTTTTTTATTATTTAATCTTTTTGCTTCTGAATTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAAATTAAAGTTAGAGAAAAAAATAAAGATAATCAATTAATCACCAAAAACTTAGAAATAGATTTTGCGGCTTCGAAATATGGTGAAGAACTTTATGTTCAAGTAGTTGATAATTTAGAAAATTTTGACATTTTAAAAAGAGAAATTAAACCTTTTTTAAAATTAAAAAATAATTATCCAAAATTTATTTTATATCGCACTAAAAATATTGACATTGACAATGTAGATGGTATCAAATAAAAAGTATTTGTGATTTCTTATTAGAAAAATAATTATTTATATATAATTAAAAAACTTAATTTAATATAGGAGGAACTATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGAAAAGTTATTGAAGCAAATAAAACTACTCCAGTAGCTGAAGTTGAAGAAGTTGAAATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGAGATGATTTAATTAAAAAAGGAATTGAACCAGGTGATACTGTTCGAATTTTAGATTATGAATTCGAATGAGATGGCGAATTTTAGATAAATTTTTAATTAAGTTTAATTGATTGATAGTATTAAAAATTAAAAACATTTTAAATGTAATATAATTTTATAGAATTATTTTAAGGAGTCCTATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGATTAAATAACGATTTATTAAACAACTTTAAAATCATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAAATTTGATTTAAAAATGACAGCATTACACTGTCATTATTTTTTTAAATATTTTTTATCAAAAATTCCAATATAAGGGAGGTTTCTCATTTTTTCATCATAATCCAAACCAAAACCAACTAAGAATTCATTTGGCACGATAAAGGCTGATTTATCAGCTTTCAATTCAACTTTACGGTTGTGTGGTTTGTCTAAAAGAGTTATTATTTTAAATGAACGAGGTTTACGAGATAAAAGAATTTGCTTGATTTTATCAAGTGTAATTCCTGAATCAATAATGTCTTCAACAATTAAAACATCTTTATTTCTAATATCTTGAGCCAAGTCCATAATGATTTTAACACTACCGCTACTATTGCCTGCTCCTGCATAACTAGAAGTAGTCATAAAGTCTAAAACATGGTCAACAGTTATATCTTTTATTAGCTGAGCTAAAAATGGGATTGATCCTTTTAAAAGACCAACAATGATTAAATTGTCTGAGTCTTTGTATTGTTGATTAACTCAAGTTGCTAATTCTTTGATTTTAGCTTCAATTTCTTCTTGAGTATATAAAACCTTTTTAATTCGATAGTCAATATTTTGCTTATTCATTATACCTCTTTGATTTTGAGAAGTCATAGCTATCTATTATATTAATAGCTTCTTGTAAAACTTCTTTTTCACTTTTGTTATTACTATTAATGATAACATAAGGTATCTTAAATTTTTCAACTAATCTTATATAAAGCTTTTTATATAAAGAATGAAGTAATTTAAAGTATGTTTCATTTTGAGCATAATTATCAACTTCTGAAGCGCGACCTCGAGCTAATAAACGAGCTTTGAATGCATTAAAATCAATATCAATAAAGATCGCCAAATCCGGATTATCTTCAGGATCAACTATTTTTTCAAACATAGCATCAAAACCTGCTTGATATTTAGGATCTTTATCCATTAAAGTGACAACAGCAAAAACATAGTGTTCGATGTTGAAACGATCTAAAAAGATATGATTGTCTCTAAAATTTAAGTTATTATTTTTAAATTTAGCTACTGTTTTTTTAAATGTGTCTGATAAGCTTTCAATAATATATGATTGAAAACCAATGTGAATATTAGGTTGTTTTTCATACATTCATTTTAAAAATGTATTAAAAACTGGGTCATTTTCTTGAAATTCTTCTAATAAAACTGATTTTTTATAATGTTTATGTAAGCCTTTAGCAAGTGTACTTTTACCACTGCCAATCATGCCACTTATACCAATAATCATTGTAAGTCCTATTTATTGTAACGTTCCATTTTACTGAAATCGTAATTGTTAATAACTTCAACAGCTTTTTCAAAAACTTGTTTTTCAGTTAAATTATCTGTTTCAATAATAACATAGTTTAAATCATATTTTTTAGCTTGTTTAATAAATAATGGTTTATAAAGATCATAAAGTTCTCTAAAGTAATCTTTGTTTTTATTTCAGTTTTCAGTTTCAACTTCTCTACCTCTTTCAAATAATCTTTTCTTGAAAGTGTCAAAAGTCATATCTAGAAAAATTGCTAAATCTGGAGTTTCATCTTTAGTAATAAGATGAGAGAAAAGTGCATCATAACCTTCTAAATATTTTTTACCTTTAGGTCTTAAGTTAACATTAGCAAAAATGTAATGTTCAATACTAAAACGATCTAAAAATATGTGATTATCCTTGTATTTATAGCCTTTTTCGCTAAATTTTTTAAAACAATCTGCTAATTTAGATGTGTGGTTTTCCACTACATAAGATTGAAAACCAATTGTTAAATTAGGTTGTTTTTCATAAAGTCATTTTAAGAATGTATTGAAGACATCATCGTCCTCTTCATATTCTTTTAACATCATTGAATTTTTGTAGTGTTTAACTAAATTTCTTGTTAGTGTGCTTTTACCACTACTAATCATTCCGCTTATACCTATAACCATAATAATTACCTCTTTCTTTATTTGTTTTTAGTCTAAAAAGTTAATAAAAAAACAATACAAAACTGATTCATGTATTAATCATTTAAATGAAATCAATTGAATAGTCAACTATTTGTATTGAATTGATAAGATTTTTATACTGTATTTATTAGGCACATCAACTTCAACTGTTGCGCCTTCATCACCTTCCATGATAGCTTGTGCTACTGGTGATTCATTAGAAATATTACCTTCCATTGGATTAGAATCATGAATACCCATGATTTTAACTGTAATTTCTTTATTTGTTTTTAAGTTAAGATATTTAACTGTTGCTCCGATACCTGCTTTTAGTTTACCTTTTTTGTTTTGGTTTGTTTCAATAACTTCAGCATTTGCTAAAATGCCTTCAAGTTCAACAATTCTTGCTTCAACAATACCTTGACGATCTCTGGCTGCATCATATTCAGCGTTTTCTGAAAGGTCACCTTGTGCTCTAGCTTCTTTTAAAGCTGCTTGAATTTCAGGTCTTTCAACTTTAATTAAGTGTTCATATTCTGCTTGATATTTTTCTAATGTTTCTTTAGCTAATATGATTTTTTCTTGCTTTTTACCTGCCATCTATCCCTCCTTATATGTTTAATAATAGTTATTTTACAATATATTAAGCTTTATTTTATAAAATAAATTTATTTTTTGTCCATTATTTGAGAAAAGCTAAAAAACGATAAATAATCGATAAAAAATCAACTTTTTAGTGATCTAAATTTAATTAAATATTTAAGTGGAATTTTTTCTTCTTTATATAGAAAAAAGATTTTTAATATAGAATATTAATTATGCATAAGAAAGGAATGAATATGAAAGTTGAATACTTAAGTGAAGCAAAAGTCGAAGAATATAAATGACTTAAAAATTTTTCGAGTAAAGTTCTTATTTTTTGAACAAAATAAGATATTTCAGGAATTAAGGGTTTTAACTCCCATTCGAAAAACTTCAAATTACATCATCCAATTTCAAAATGCCTGAAGTATGTTCGCGAATTTTTTTGATTATTGTTGTTGCTTTCATAATGAATAAATTAATTATTTAGTTTAGTTTTTGTTAAAAAAAGAACATTTTCGTTACAAAAAAGTCCTTAGTTTTCATTTTTTATTTCAGTTTTACTTTTTACTATATATAAGAATCCGCTTTTTACATAACTTAATTCATAAACAATTTTATTAAGTTTTAAATGTTTAGTTAAAACTCTATAACCACTTTTTGTATTTGAATTTTGTAATACAACAAGCATCCCTTTTTCACTTAACATTGGATAATATTTGTCAAATAATTCTTGATTTGTTTCAGTTGAATTAATTGAAAAAATCAATTTGAATTTCTTATCTTGAGTTTTTAATTCATTAACATTAATAAATGAAGGTTGATCTTTAAAGTATTTAGGTAATTCTAAAACAGCTTTATTTCATAAACTTACATTCATGTTTTGTTTTTCTAAACAAATTTGAGCTTGTGATTTATTTTTAAATAAAGCTGTTGGATATTCTAACTTACTTCCAACAAGTAAAATCTCTTGAACATCATTTAAATAAATAGTATTAATAAAGTTTTCTAAATCATAATCATCAAGATTACTTTTAAAAAATTCTTTTAATTCTTTAGGGAATTCTCCTAATTCTTCACCTCTGATTTTCAAAATAGCTTCATGAGCTTTTTGAGCTTCTTTGGTATTTATTTCATTACGAATTTTTCTAGTTTTAACTAGTAAAATAATATAAGTTATAAAGGCAATAATCACAAAAACAATAGCAGAAACAATTAAGCCAATTTTTGCACCTTTACTTAAATTGATTACAGCACTAATCATGTAATTCTACACCTCCATAATTTAAAAATTCTTGCAAGATTAAAACAGCTGACAAAGTATCTTTATGAGCTTTTCTTTTTTTAATAGAAAGTTTTGCTTCTTTTAAAATATTAGTAGCAGCTATAGTAGTTCCATATTCATTAACTTGATAAATTTTGTAGTCTTTAAAAATACCTTTTAGTTTATGAGCAAAAGATTCAAAAAGCACTGTTCTTTCACTACTTGTATTATTAGATCTTAAAGGCTTTCCTAATACCAATTTATCTATTTTATATGTTTTTTGGTAAATTTTGATTTGCTCAATAATTGCATTAAAATCATTTTCTTCAAAATGTAATGTTTCAAGTGCACTAGCTATAATCATGCCTTCATCACTAATTGCAAAACCGCAAGTTTTAGTGCCTAAATCTAGAGCTAAAGCTCGCATTAAATAAGTTCCTTAATAATATTTTCAAGATCTTTAGTATAGTCAACTTTACCCATTGAAATAATTGAACTTCCACCACCACGACCATTGGTTTTTGAAGCTATTTGTTGGAATAATTTGTTAGCATCAAGCACTTTAGCGGCCACACATAAAAGAATTTGATTATTACTTTTAGCACCTAAAACAATTATAGCTTCAGGAAATTTTTCTCTAAGCGTTGCAGCAGCAACTTTAACTTGGGAACTATCAAAATCAATGTTTAAATAATAAGGGTATTTTTTGTTTTTAGTTAATTGAACACTATCAAAATCAAAGGTTATATTGCTTTTGTTTTTGATTAATTCTTTGATATCTTCATGAACTTTATCAATTGTTTTTTTAATGTTTGCACATTCAATTTCAACATCTTTATTAGGTGTAAATTTAATTTGATATTTACTATCAAAGCTTTGAGCTTTTTTAACTAAAGCATCTAATTGTTCTTTTAAACTTTGATTTAATTCTGTTAAATATTGTTTAACATATTTATGAGAAGCTACAGCTCTTATTCTAAATACACCAGCAGTTTTTTTCTCAACTTTAGTAATTTTAAAGTCTTCTAAAATTCCGGTTCTATCTAAGTGAGTACCACCGCAAAGGTCTTTTGTAATACCTTTAAAGTCAACAATACGAATGTTATTAGGATCCATGTATTCACTTTCGTCAATAGTCATAATTGCGCCCATTTCTTTGGCTTTTTTAATGTTTGTTACAATATATTCACGTTCAACATTTTGTTTAATTCAACCATGCATTATGTCTTCGATTTTTTTAATTTGTTCATCAGTTGGTTTTTCATCAGCTGGAAAGTCAAATATTAAACGTTCTTCGGTGATATCTGAACCAAGTTGATCAATATGAGGTCCAAGTACTTTAACTAAAGCACGGAATAATAAGTGAGTTGCTGAGTGACCTCGAGCCATACCAATTCTTACTTCTTTATCAACATAACATTCGACAGTATCCTTATTGTTAATTTTGCCTTCAATTTTATGAATATGGTTACCAAATTTGTCTTTAAAAACATCTAAAATTTTGATTTTATTTTTACCTTGTAAAATGAAACCATGGTCATGATTTTGACCACCACTAGTAGCATAAAAAGGTGTTTTATCTAAGATCAAATAACCTTCACCATTTAATGATTTTACCTCTTCCTCATTATTTAAAAGTTTAAGAATTTTTGATTTACTTTCTAAGTCTGTATAACCAATAAATTCACCGATTTTGCCTTTAATTAAGGCTAATGAGTTAATTACTTTATCCATTCCGTTAACTTTTTCACCCCTGCTTAAGTTGGCGTGTCTTTCCTTAGCTTCTTCAAAAGCTTTAAGATCAACTTTAATATTTTTCTTAGCTAAAATTTCAACAGTTAACTCAATTGGAAAGCCATAAGTTTCAAGTAAATTAAAAGCATCTTCACCTGAGAAAATTTTAGTTTTATTATCCATAAATTTTTCAAGTAATAATTTACCTTTTTCAATAGTTTGATGGAAAGCTAATTCTTCTTCTTTAATGGCTACTACAACAGGTTTTTTGTCATATTCATAAGGTAATGAATCTTGTACTACATCTACTAATTTATGTAAAAATAGACCTTTAACTCCTAGTTGCATTCCTTTGTAAATTGAACGTCGAATAAGTCTTCTAATAATGTAACCACGGCCAACATTTGAAACTTTAGCTCCATCAGCTATAGCATTAACCACTGTTCTAATGTGGTCTGCTATAACTTTAAAGTTAGTGTTGATTTCAGTTTGCTTTTTATCTTTTTTAAAGTAGTTATCAATGTCATATTTAACTGTGGCATATTTTTCTATTTCTTTAATTATGTTAATAAAAAGATCGCTGTCATAGTTAGTTGGAGCATCTTGCATAATTGAAGCAATTCTTTCAAGTCCTGCACCAGTATCAATATTTTTTTGTTTTAATTCAGTATAGTTATTTTCACCATCATTATTGAATTGACTAAAAACTATATTTCAAATTTCGATGTAACGGTCATTTTCAATATCTTTTTTAAGCAACTCTAAACCACGTTTGTTATATTTAGGTCCTCTATCATAAAAGATTTCTGTATCGGGTCCACAAGGTCCACTACCAACATCTCAGAAGTTTGTATCTCTAGTTCCTGGAATTAAGTGTGATTCTTCAACTCCTTGTTCCATTCAGTATTTTTTAGTATCCAAATCTTCTGCAAAATAAGTCATGTAAAGTTTTTTAGGGTCTATTTGTAATTCATTAGTTAAGAATTCATAACCAAAAGCGATAGCTTCTTTTTTAAAGTAATCGCCAATTGAAAAGTTACCTAACATTTCGAAAAATGTGTGGTGTCTTGAAGTTACACCAACGTTTTCTATATCATTAGTTCTAATTGCTTTTTGACTGTTAGTTAATCTATTTCTAGGCGGTTTTTTCTTACCTGAAAAATAATCTTTAAGTGTTGCTACACCTGAGTTAATTCAAAGCAAAGAAGGATCATTAACTGGAATTAATGATTTGCTTTCAACACGCAAGTGACCTTTTGATTCAAAATACCTTAGTCACTTTTCTCTAATTTCTTTTGATGTTAATTTTTCCATAGTTATCTCCTAAATGTAATCTTTTTTAATATTGTTTAAGTATATTTCTTCAATTATTGCTCCGCCTAAGCACTTGTCTTGATCATATAAAACAACTTGTTGACCGGGGGTTACTGCTTGAGCTTTTGATGGATAAAATACTTTAATTTTATTATTTTCTAAGAATTCAACTTTAACTTTAAGATCTTTTTGACGATATCTAAATTTAGCACTTAATTTGTTTTTATTAAAGCGATGATGATTTAGACATAAGTTTGAAGCTATTAGCATATTACTTTCTAAATAACTAGGATCACTAGCTGGTGCAGCATAAATAATATTGTTTTTGACATCATGACCGCAAACATAGTGAGCTTCACTTTTGCCGCCTAAATTTAATCCTTTTCTTTGACCGATAGTGTAATAGAAACAACCATCATGAGTTCCTAAAACTTCTTTAGTTTTAATGTCTAATATTTTTCCTTTTTGAGCTGGAATATAGTTTTGTAAGAATTTTGTAAAATGTCTTTCGCCAATAAAACAAATACCTGTTGAATCTTTTTTTGTAGCTGTAGCTAAGTCTAATTTTTTAGCTATTTTTCTTATTTCTTCTTTAGGATAATCAGCAAGAGGCATTAAAACTTTTTCTAGTTGTTGATTAGTTAATTGAGCTAAAAAATATGTTTGATCTTTATTTTGATCTTTCGCCCGATAAAGTTTACCATTCTTAACTTTGGCATAATGACCCATGGCAATATAATCAGCATTGAGTTTTTCAAAAGCATATTTTAAGAAAGCATCAAATTTAATATATTTATTGCATAAAATATCAGGATTAGGTGTTCGACCTTTTTTATATTCTTCAATAAAGTTTTCAAAAACATTATCTCAGTATTCTTTGACAAAATCAACACGATGAAGCTTAATACCTAATTTATTTGCTACTGCTAAAGCATCTTGATAATCTTGTTCTTGAGGGCAAATATCTTGACTAATATTTTCATTGCCTAAAAAATCATTATTAACATATGAATCTCAATTCCGCATAAAAAGACCTTCAACATCATAACCTTGTTGAAGTAATAAATATGCAGCTACTGAAGAGTCTACTCCTCCAGACATTCCTAAAATAACTTTCTTTTTATGCATATTAATCCTTATATTAAATATTAGTTTTAATTATATTATTAATTTTTATATTAATAATCATTTTTTAAGAAATGTCAAATAAAACCACACAAGTGTGTGTCTTATAATTATCCTTCAATTCACCTAGGTGGGTAATTTGTTTAGTTTTTAGCATTTTATTTTATTTCTATAATTTTATTGTGAATTTCATTTTCGATAAAGCTTTTCCGCTATTTTCTATAGTAAATACTTGAGCAAACTCATCATAGGTATCTTTAACATTTTATTTTAAAATATCTTTTAATGAAGCCATTTTTACTCTGTTTCTATTAACATCTTCATCAGTTAATTTTTGTATTAATTTTTTTAAAATAAATCTTTTAAATGTTATTAGAATTTTATATCTAATAAAATTACTAAATAGACTGTTTTATAGTTAAATTGGACAATTTTGAGTATATTTTTCAAATCAATATTAATCAAAAAATAAAACAATCAAAATTAAACATTCAAACAGTTGATATATAAGTTTAAGTTAATTAATATTGCCTATTTTTAATTTAGTTTTTTATTGTCCCTTTCTATTTTTAATCAAAATATAATTATTATTTTTTCTGAATCTATTAAAAAAACATAATTTTTTGACTCTTTAATAGCGAATTAGAATTATAAAAAAGAAGCACTATTGAGTACTTCCTAAAATTATTTTGAATTACATTTTGTGTTTTGAACTAACACCTAAAAGTCTTAATCCGTTTTCTAAAACAATATTAACAGCACGAGCAATAGTTAAAGAATTCATTTCATTCTTGCTTCCAATAATTTTTTCTGTATTTGAGTATCATGAGTTAAATTCTTTGGCAACTCTAATTAAGTATTGTGTTAGTAAGTGTGTTAAGTACTTAGTTGCTGAACTAATGATAACTTCTGGAAATTCAAGCAATGTCGAAACTAATTTTTTATCAAGTTCGTTGTCAATATTTGTTGCTTTAAGTTTTGAAACATCTAATTTAGATTTCTTAAGCAATTGGTATGAACGAGCATTTGCATATTGAATTAAGAAGACAGGGTTGTCATTTGATTTTGAAGTTGCAATGTTAATGTCGAAGTCAAGTTTTGAATTATATGTTCTATCTAAAAGAATGAAACGAGCACTGTCTACTCCAACAAGGTCAACAAAGTCTCTTAATCAAAAACTTGTACCTTTACGTTTTGACATTTTGTATTCTTGACCATCTTTCATAAGTCTTACTAATTGTAAACAAAGAACAATTAATTTGTTTGGGTCGTAACCTAAGTCTTGCATTGCTATTTTCATTCTAGTGATGTAACTTAAGTGGTCAGCGCCTCAAATGTTCATAATAATTGGATCTTTGCCTGAGTTTTTGAATTTTTTGTCATGGTAAGCAATATCTGGTGCAAAGTAAGTAAAATCACCATTGCTTTTAATTACAACACGGTCTTTATCATCACCATATTTTGTTGTTCTAAGTCATGTAGCACCATCTGCTTCAAAATGGTTTTTAAGTTTTTTAATTGTGTCTAAAATTCTTTTACTGTTGTTTGCATATAAAGTTTTTTCACTAAATCAAATATCAAATCCACATTTAAATTTTTTAAGATCAACTTTAATTTGTTTTAAGAAATTAGCCACACCTTCTTTTTTAAATACTTTTAAAGCTTCATCATTTAATTCTTTAAGATCTTTAAATTTATCTCCATGTTTTGTTCTAATTTGTTCAGCATTTCAAACAATGTCTTGACCTCCATAGCAGTCTTCTGGCATAGGGAAATCTGGTTCATAAAATTGTTTATATCTTGCAAATATACTTTGAGCTAAAGTGTTAATTTGGTTACCACCATCATTGATGTAATATTCACGTAAAACATTTAAACCTGCGAAAGTACAAATGTTGTATAGTGCTGATCCAATAGCAGCATTACGTGCATGACCAATGTGCAAGTATCCAGTAGGGTTTGCTGAAACCCATTCGATGTTTAGATTTCCTTTTAATTTACCACGACCAAAGTTTTCATTTTGTTTGATAATTTGGTTCAAACTTTTAATAATTGTTTGATCATCAATGTAAAAGTTTAAAAATCCAGGAGCTGCAATTTCTACACTTTTTAGACCTAATGAAGTTAGTTTAGGCTCTAATTCTTTAACAATTTTTTTGGCAAGTTCCATAGGATTTTTGCCTTTGTTTCCTAATGCAACGTTAGAAGCGAAATCGGCTTTGACTTTAGGTTCAATTAAGAGATCGTCCTTTTCAATCCCTAATTTGTTTAAAATTGGTTTTAATTCTTTAATAATTTTGCTTTTAATAATGTTTTTATCCATAATGTCAATATTATAACAAATTAATAGTTAAAATATATGCCTATTTTAATATTTTATTTTTTTGTAAAAATAAGTATTTTTTAAGGTTGTTTTTGCTAATTAAGCTTTTTTATAGGTTAATTGAAAAAACAAAAAACTTCACACAATAAATAATGGAGATTTTTATATGTGCAAAGTTTTTCTTTTAATTTAATTGAATGAAAAGTTTTTTTGATGTTACTTATTAAAAGTAATCTTTCAATATTGCATCTTTATATGATTCAACTTCATTTCATAAGTCATTTAAAATAGTATTTATTTCTATTATTTTTTTGTCTTTAATTAATTCGCTGTAGTAATTTTCAGATTTCATTTTTTCGCCAAGTTTTTTAGCTTTATCTCTTTTAGCAATAATTGCAGAATCAGTTATTTTTGTAGTTTTTTGATACTCTTCATAAGAGTCATTTATTTTGGCTAAATATGCTTTAATGTTTTCAAATTTAACTTTAGCAGCTTCTAAGTATTTTTCTTCATCAGTTTTACCATCTCCACAAGAGACACAAACAACTGATACAGGTAAAAATGAAGCAACTGCTAAACTTGATATTAATAGTTTTTTCTTAAACATACTTTCTCCTTATAAATTAAGCTTGAACTTCAACTGGTTTTTGTTTAGTTAAAGTAGCTTCTTCTTTTGACAATTCTGCTAATTTTTGTGCTTTGTATTCTTCATAAGTTTCTAAAGAACCATATTTTTTCTTGTTAATTGCATCTTCAATAACTGCAGGAAGGAATGTTAACATTGCAAATATTCCTAATACACATACTAATGCAACTCTAGAAATAATTAAGTTTTTGTACATTTCAGCTCTTGAAGCATCAATAGCTTTTACATATTCTTCAAGTGTTGCATTTCCTAGACTACTGTCTACTGAATAGTCAACATAATCTTTGTTAACGCCTGCAAGTAAGAACATATCAGTAATAGGAGCTAAAATAGTTACAGCTAAACTAAATCCAACTAAGATAACTGAAATTCATGCAAATGGTAAGAAGTATTTTTTCTTATTTTTAATTTCAATTTTGTTTGTTTTTCTATTTTTAATTCCGCCATAAATTGCAACAGCAATAAATCCGAATGTAAATAAAGCTGTTCAGTTAGCCATCAAGTCGCAGAAGGCATATAGTCTTGCCATTGATTGATGCGCATCACTACTATAAATTGTATAATCTGAGTTATCAGCAATATATGCTAAAGCACCAATTGTAGTAAAGATAATAACAACAGGAATTGTGATTACTAGTGAGTATATAATACCAACATATGGTTTGTTAGGATTTAATTTTTTGTGTCATTTTTCTCAGAATGGTAAGTCGCCTGTAGCTAATAAATCTTCTACATAACGAGGAGCTCACATTGAGAAACCATTAATAATACCTAGAACACCAATTGAAATACAAATGTTAATTAAACCAAAAACAATTCGACCAGCTTTTTCATTTTTGAATAAACTACTCATGTATCCATTGAAGCCAAAGAATGATCCACCGTTAAGTGACATTGAAATAGCAACAATTAAGTAAATTAAGGTTGTGATTCCTAAACCAATGAAAATTGCTAAAGGAGTCTTTTTAGGTTCTTTCATTTCACTTTGAATTCCAGCAGCGACATAGAATCCATCATAAGCAAAGAAGATAGCAGCAACAGCAAGGAATACACCAAGACCAGCTCCTAATTTAGGTAAAGCAGTAACACTTTTACCTGATTTAATTGCTTCTGCAAATGTTTGATCTGGAGCAGGCATTGTTCAGTTAACTTCGTTTAAACCACCATTTCCAGTGAATGCTAATACAAATCCCATAATAGCAATAAATACTAATGGTATAAATTTAACACTTAAAACAACAATGTTTTGAACACTACCAACTTTTGATCAAATTGCAGGTACTGTTAAGAAGTAAACAGTCATTAATAAGCTAATTGATAATCAAATAAGTCAGTCAGCATTTGTTCCGAATGTTAAAGCATCTTTATTCATTAATGCCCCAACACCATCTTGAAGTGACATGATTACATACATAGGCATAAATAAGTATGTAAGAGGTAAATAGATATAGGTCATAAAGTCTTTTGAAGCGCGGAAAATCCGTTTGCTGTTAAAGACTTTACATCAGCTCATAAGTGACAAGTTGTCATTTTTAACTGATGAAATTTCAATCAATGATAAAGCCATTGCAATAACCGCAAATGATGCAATTATTCAACAGAAAATGGCTAGAATCAAACTACTGTGCGAGCTTTTTAAAACAGCACCTGATTTAAAGAAGATACCAGCACCAATTGATCCACCTATAACGATCAACATAGCAGCTATAAAGGAAATCTTCTTTTCTTTTGTTTGTTTTTCTTCCATTATTAAATTTCCTTTTTAGTTTTTTAATAAAGTTTTATTTTTTATTTATTGAATTATCCTTTGATAATTCTTGTACCACTTTTGCCTTCGATAGCTTGTGATACTTTGTTTAAGTCAGCAATAACAGCTACATTTTTTGGATTTTCGTTAACAAAAGCAATAGCAGCTTTAACTTTAGGAAGCATACTTCCTGGAGCAAATTGTTCTTCTTTGATGTATTGTTCAAGTTCAGAAACAGTTACTTCTTTTAAAGCTTTTTGGTTTGGTTTACCAAAGTTAACACAAACATGTTCTACTGCTGTAAGAATCACAAATAAGTTTGCTTCAACTTTTGTAGCCAATTTACTTAAAGCGAAATCTTTGTCGATAACGCCATCAACACCTTTATATTGTCCTTTTTCGTCGATAGTAGGTATGCCACCACCACCACCAACTATAACAACATTATCAGCGTCAAATGTTTTTCTAATTGTTTTCATTCCAATAAAGTCAAGAGGAAGTGGTGAAGGAACAACTTTTCTAAAACCACGACCAGCATCTTCTTTAATGGTTGAATTAGGATTAGCCAATTCAGCTTCTTCTAAAGTTTTATAGAAAGGACCAACTGGCTTTGTAGGGTTTTTAAATGCTTTATCTTTAGCATCAACTATTGTTTGAGTCAAAAAGTATAAAACATCTTTTTCAATTTTTTTCTTTCTCAATTCATTGGTTATCGCTGTGAGCATGTGATAACCAATGTAGCCTTGAGACATACCACCTGCTTCTGCAAAAGGTACTAATGGAGTTTTTTCATTAACTTTTTTTGCATCAGCAAAGGCATTGAAGATCATTCCAACTTGAGGACCATTACCATGACCAACAAGCACTTGGTGACCTTCTTTAACTAAATCTGCTATTTTTTTAGCAGGTAATTTAACTAATTCTTTTTGTTCTTCAGGATTGTTTCCTAAAGCGTTTCCACCTAAAGCTATAACAATTTTTGACATAATTTAAATCTTTTAAATTTTAGTAATTGTACTGTTTTATAAGACTATTGGGAGAATTAGTAACCAATAGTTGCTAAAACAACAGCTTTAATTGTGTGTAAACGGTTTTCAGCTTGATCAATTGATCTGTTGCATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCTCCAGTTGCAACTACTGGGTATTTTTTACCAAATTTTTCTTTGATTTCAGCTGAGAATAATGTGTGGTCATCGTGGAAAGCTGGTAAGCAGTGTAAGAAGATAACGTCTTCTTTTGCAGCTTTAATCATTTTCATATCAACTTGGAATGCTCCTAATTCTTTGATACGAGGTTCAAATAATGAGAAGTCTTCTCCTAATGATACTCAAACATCTGTATAGATTGCATCTGCATCTTTAGCAGCTTTGATTTTGTCGTCTTCAAATGTAATTGATCCACCATTACGTTTGAATAATTCTAGACATTTTTCGTAAACTTCTTTGTAACCTTTTCCAGTTTTTACAATTTTTGCTTGTTCTTTAGGTCCGCATAGAACACAGTTCATTCCTGTAAAAGCAGCACCAATCATTATTGAACGAGCAACATTATTTTTGATGTCTCCAGCAAAGACAATTTTTTTGCCTTTAAGATCACCTTTGAATTCCTTAATTGTCATATAGTCTGCCAACATTTGTGTTGGGTGTTCGGCGTCTGTAAGTCCATTTCAAACTGGAACTCCAGAATATTTAACTAACGCATCAACATCAGATTGTTTGAATCCTCTGAATTCGATACCATCATAAAATCTTCCTAAAACTTGTGCAGTGTCTTCGATAGATTCTTTTTTACCAAAGTTAGAACCTGCAGGTCCGATATATGTGCAAGATGCTCCTAAGTCAGCTGCTGCCACTTCAAAGGCGCAACGTGTTCTAGTAGAGTCTTTTTGGAACATGATAGCAATGTTTTTTCCAACAAGTGGACGATTGTTAGCATGTAACCCTTGTCTTTTTGATCTTTTTAAATCAATAGCAAGGTCAATAAGTGCATTAATTTGATCTGTAGTGAAATTTAGAGCTGAGTCTAAACTCCTACCTTTTAAGTTTAATGGCATATTTTCTCCTTAAATTAATTAATAAATTTATTTTTTTGCTAAATTGGCACTACGGCGCAAATTGAGCTGATAAAATTTTATCACTATGATTTATTTTTTCTTAATGTCTTCTCTAATAAGAGGCATTGACATACATCTTGCTGAACCCATGCCTAATGAAAGTTGGTTTCCTTCGAATGATAAAACTTTAACTTTAGCTGCTTTAAGAGCTTTTTCAGTTTTTTCATTTCTTGAATATCCAACTACAACACTAGGTGCAATTGTCAAGTAGTTTGTACCATCGAAGTGAGTTTCGATATCAATATCTAATTGACTTGCTCCTTTACCTGCGATAGGAATTAAGATAGGTTTCATACCAATAATTTGTTCAAGAATTTCTTCTAATGAACCTTCAATTTCGTGTCATACAAAATCAGGGTCATTTAAGTCGATTCTTCAAACTTTTAATACTGATAACATGTTTGGTGAGTATAAGAATTTATTCTTATCTAACATTGTTAATCATGTATCTAAGTGCATTAAGTTTGGCATAGGAGGAACATTGATTGCATAAATTCTTTTGAATTTAACTTCTTTGTCCGCTGCTATTTTACGAGCCATAACGTTAATAGCTTCTTTGTTAGTTCTTTCGCTAACGCCTACTACTAATGTATCTGCTGAGTAGATAAATACGTCTCCACCTTCAATTCTTCCTTCATCTTTTCTGTCGAATCAACGAGGTACTGTATTGTAATCAAGGTTATTGTCAAAGATAAATTCTGAGAATATTGTTTCTCTTTGTCTTGTGACATATTTCATGTGGTGTAATGAAATACCATTACCAACTGATGCAAATGGATCACGTGTAAAGTATAAGTTTGGCATAGGGTCAACTACAAGATCTCTATCTAATTCGATACCAAGTTCTTTTTTGTCGATACCAGCCATCATAACTCTAACCATTTTTTTAGATGATTTAGCTTTAAGTTCAAGAATGTATTCTTTTACTTTAGCTCTTAATGTAGCATCTAATTTTGGTTCGCATTCATCTAATCATCTTTCAATGAATGATTGCTTAACTGATTCGCTAACCAAGTCAAAAGTTTTAGCGAATAATTCGTCCAATTGAATAACTTTAATTCCGTTATCTTTTAATAATTGAACAAATCTTTTATGTTCCGCAATTGCAGAATCTGGTTCCAAAATTGCTGAAAATAATAGTTCATCGAGGCGAGAAGGTGCGATGCGCCTAATTTCGTCTCCAGGTGTGTGAACAAGTACTTCCTTAAGTTTTCCGTATTCACTGTAAACATTAATTTTTTTCATAATTAATCTCCCGATAAAATAAATTTTCATGAATATTAACATATTTGTTAATAAATCAATTTTTGCAATGATTTGTAAGCGTAGCTTATATTTCTTTTAGATATAATACGTTAGTCATTGCATATAAATTATAATTTTTTAATAGGTTAAAAAGGCAAAATTGTGTTAGTTATGGAAATTTTCTCACATTTTGATACATATAATAATATTAATTCTATTTTTAAGTATTAAATAAGCAATAAAAAAGCAAAAAACATAGATATTTATTCGATATCTATGTCTTCTTATTTGTATATTAGTTGTTTTTGATTACTAATTCTAAGCTATTAGGATCAAAATCATAATATTGGTTTTCCTTTAATTTATACTCGTTTTCGTAAATTTTAGGAAATACACTAGCTACTATTTCCATAATAAAATCATATGTCATAATCATAGCCATAACATCATTTTCACAGTATTCTTTTAAATATTCAATTTTAGCTTCTCAAGCTTTGTCACCAATGATGCCTAATTTACGACTAATTGATTCTTCTAAAGCCATTGATCCATTTTGGATTTCAAGAGTTTTATAAGGTTTAATAATATGTCTTAATTTAAATTTATTTTCAGTGATAATGTGTTCAATTTTTTTAATACTGGTTTTGCCTCATAATTCATAAATGAAAGTTGAAAATCTTTCGTCTTTATAGTAAGTTGCTAAATTTTCAACGTTAAAAGGAATACTATTTTTAGAAACTGAAGTATATAAAATTTCTTTATTTAAATTTTCACTATGAAATTTAGCTTGAGGGACATAATTAGTTTTAAAAACATAATAATTTTTATTAGCATCCATGCCATAACGTTCATCGAATTTATTCTTAGATATTGGTTTAAAACAATCTAATAAGTCAATAGTATGTTCATTAACATAAGTAATTAATTGTTCTAATTTTTCCATATCGAGCTTATTTATAGATAAATAGTGTTCCATGTCTTTTTTATGTTGACTATCAGCATAATAAAGTTTAGCTAATTCTATAAGTTCATTGTTTCTGGTATTTTCATAACCTTTGTTATAAACAATGTAGTAGTCAGCTTTATTTGAATAAACAGCAATAATATTGTCTAGAAGATCATAAATCGTGATATTTTTAGGGTCTTTAACAATATTATTTTTATTTGTTATTTGTCCATTAATTGTTTCAATAATTGAAACTTGGTGAGTGATTTGAATATAAGGATTCAAGTAGTCAATTATTGGAAATACTGAGCTAAGACCTTCATAGTCATATCAAATAATTCTTTTGTCTTTTTGGTGTAATAAAGATAATATTTCTAAAACAAAAGATGAAAAGTTATTATGGAATGATCTTTTTTCCTTAACTTTTTCTCAAAATTGATTTAAGTATTCTTGATCAAAAGTTTTTAAACATTTTTTGACAGCAGGAGAATATTGTAATTCAAGAGGTGTAACAGCTACTCTAACTTTATTCATTAAATCTTTGTTTTTACCATAAAACATATCTGAATCCGGCAACATAATTTGATTATCAAATGAAAATGTTGGTTTAGTTAAATAATAAGCTTCTACAATTTTACTTATTACTTCTTTAAAGTCTCCAAATTTACTTCTTGGATCTTTGCTCATAAATTCTAAAGAAGTTTTTGGCATTTTGCGTGCACTTGGTTGAGTGAAAATATCTTCTTGAAGTGCGGCAAAAAGGTAATCTCCATGAACATTAGCTTCATTTTTTGAAGCAAAATAAGAACCATCATTAATTGTGTAACCAAGATCACTTCTTAAAGCCTTTTTGTCCATTTTGTTTTTACCACAACTTGAAGCTTCAATTAATCTTAAGTTTAGTTTATCTTTTCTAAAAACAAAACCATTTTGAGTATAGTCATCAAAAATAATATTTTGAATTTTTTTAACTTCAACTTTGGCTTTTTTAGCTACTTGATATAAGTAATTAGCTTCTAAATAATCTTTATTAGTTGTTTTAGAATTCATTCTTTTAAAATAAATAATTTTATTAACTTTATCATAAGCAAAAGGTTGGGCTTTTATTGTAAATTCTTCTTTATTTTTGCTTAAAGCTTTAAAAGAAAAAACAGGATTAATAATGTATTTAATTTTGCTGTCTTTTAAAGCTTCCTGTGTGTTAATTGTAGCTTCTTTAACGTCTGCTACTTTTTTGAATATGGCTGTTTCATAGTTGTTTTGGACTTTTCAAATGTTATTTTTTAAGAAGTCGATATATTGAGATTCAATCTTATCATAAGCTATTAAATTAGCTCCTTTAAAGTCTCAAGGAGTGTTTTCTGCTTGATAAACAAAATCACTTCAAAATGTTTCTTCTTCACCTTCTTCACTTTCATCATCGCTAAATTCAGCAAGGTTTAAAGAATGATAATTTTTAATTAAAGGCAATGAAAAGTTGTTAGCATCTTTATTTTCTAAATCACTATCTAAAAGATTGTGTCAAATGTAGTATTTTTGCTTAACAAAAAATTTGTAATATTGGCCAAAATTAATTATTTTTTTAATTGAATTCATAATCTTCATCAACTTTTCCTAATGATTTTCAACGATCAAACAATTCTTTTCATTCAGCTTCATTATGGCATCTTAAATAATCAACTAAATCACGATCTTTAGTTAAATTGTATTTAGGAGATTCACCCAATTGTGAATATCTTTTTCAAAATTCAATTATGGTATTTGCATCATCTTCAGCCATTTTTTTATCAACTTTAAAAGGATAAGTTGCAAAATATCTTTCATTAATTGGAAAGTTTTCAGGATCGGCGTAGTCTTCTTCTTTTAAGAATGCAGCTGCAATCATATATTTGTCATAGCCTAATAAATAAGCATAAAGTTGGGCTTGTTTTTTGTAATCAAGAGGTACTTTATTATTGGGAGCATCTCAATCAACCTTTTTCTTTTCTTGAACAGTTTTAACTTCAACTACAATATTTTTTGAAGGAATAAGACCATCAGGAACACCACCTAAAATGTCGTGTTTGCCTTTAAAATAGTCATATCCGACATTTGCTGCTTCAATATGAATTACTTTTGTTGGTTGACTATCAGCACTAACTTTACCTCTTTTAATATCTTTTTGATGCATTTCATTAAATTTATTTTGCATAAGTTCAAAAACTTTAGGTTCTATAGCTACCCCGGCATCTATATATTTAGTTTGTAACACTGGCATACTTAATCTTGACATAAAACAAAAAGCTTGGAAATGACTTTTGAAGCTATCTGTTTCTAAGACGTTACCAATTGAGCTACCACCAAATTTTTTGAAACCATTAAAAGTTCCTAAACTATTTAAAAGTGTGTTATGAAAATTGGTTGTTAATTTAATTGTTTGTTTTCTTTCATCTAAAATGTAATCTTGTCCATTATAGAATTTTCTTTTTGTTAATTTTTTAACTCTGTTCATTTTTATCTCCTTTTAGTTTGTTGAATTTTCTTCTTTATATATTTTGTCTAATCAATATTTTATAGCAAAAATTAAGTCTCTAAAAGTTTCTATTCTTTTTTCAACTTTAGAATCATTGTCATCTATTGATTTTTTATAAAATAAATTATTGCAAAGTTCTCGTTGTAAAAATCGATGAACAACATTATTTCAAAGAATTGTTAAGATTCTTGAATCTTCAAATGAATCATGCATATTCAATTTTTCAAATTTATATTCTTCTTGAAAAAGTATATTTATTTTTTTATCTTTATTTTTTAATTCTTCTTTTAATTCTTCACTAAGTAAAGCATCATCATAAGTACAAAAATATTTTGCCACATTAAATAATGTTTGTCTATACATTGTTTCTTTTTCTTTACTAATTAATTTTTTAATTATCATTGTAAAATTGCAAACATCAATATAATTGACTAATCGACTTTCAACATATTTTTTACCTACAATTTTTTTCAAGTTGTTTAAATCGGCTTTAATGCCATAAGAAATTAAGATTTTGTTAGCATCTAAAATATTTTGAACTTCTTCTCTTGTTATTAAATTTTGACTTTGATCTTGATAAATATTGCTATATTGATCTAAAATGAAACCATCAATTTGGAAAAACTTACGGTTGTCTTTAACTACACCTTTGTAAAAGTTTTTTCTTTCTTTTTCATTAAATTCTTTATTGTTTAAGTTTGATATAAAAGAAGAATATTGAACTGGATAGTAACTAATTGCCACTTTATCATTTAATTTTGTTAATCTAGATTCAAAATCGAAAAATAAGAAGTTTTTATTGTTGAATATTTTTTGATCTAAAAATTCATCGTCGATTCTAATGACATCTTCAACATTAATTTTTTCTAATTCTTTAACACTTGTTACATTTGTTTTATTTTCTTCACATTCAGATATTTTTTGAATAATAGCTTTATAAGGCATTTTATGTCAATTTTTATTTTTTGAAACTGTATAAACAAAACTATATTTAACTAAATCGTTTTGTCTTTTGTTTGCTTCATTTTGTTTTTCTTGTTTAGTTTTAGTTTGTACTTTGAAACCAAATATTTTTTTAATTACAGAATATGATTTTTCTTTATCAGTTTTATTTTTATCTTTTGGATAATAAAAGAATTTAAGTTCAAAGAAATCATTAGTATCAATTTCATCTCCAACTATTAAAGAAATAAATTGATTATTATCTTTATCAGCCGTTAAGCTTTTATTAAATATGTAGCCTTGTGTTTCATAACATTGTTTTTTAGAAAAAATACTTGGGTTATTTTCTAATTCTTCAATAACTTCGATAAAAGTCTTTTTAATTGTTCCGTTTATATTGCTCATATGTCTCCTTTTTTACTAATAATACTATAAAATAGTATTTTTCAATGATAATGGAATAAATAATCATATATGAGCATATGTTAAAAAATCTACCATTAAGGTAGACTAATTTAAATTATTTTAATTTTAAAGCTTTATTTAAAAGTTTAATAACTTGTTCTTCACTATCTAATTCAATAGCTTGAGCAGCCAATTCTTCCATGTCTTTTTTGCTTAATTGAGATACCAATTCTCTTGAAGCTAAAACATTACCAGCAGACATACTAAATTCATCTAAACCTAAACCTAAAAGTAAAGGCAATGCGCGTCTATCTCCGGCCATTTCACCGCACATACCAGCCCATTTACCATGTTTGTGAGCACCATCAATAACCATTTTTACTAATCTTAAAATAGAAGGGTTAAGCGGTTGATATAGGTATGAAATAGTTTCACTCATTCTATCTGCAGCCATTGAGTATTGAATTAAGTCATTAGTTCCAATCGAAACAAAGTCTGCATATTTACAGAATTGATCGCTTAATACAGCAGCAGCAGGGGTTTCCATCATAAGACCTACTTCAATATCCTTTTTAGGAGCAATTTTGTGTCCTGCTTTTTTAACTTCACTATAAGCTTCTTCATAAAGTTTTTTAGCTTGCAAGAATTCTGGAATGTTTGTTATCATTGGGAACATAATAGCAACTTTACCATAAGCACTTGCTCTAACTAAAGCTCTAAGTTGAGTTTTAAAGATTTCAATTTCTTTAAGACAAAGTCTTATTGCACGATAACCTAAGAATGGGTTCATTTCGTGAGGGAATGTAAAGTATTTAAGAGTTTTATCACCACCAATATCTAGTGTTCTAATAACAACTCTTTTACCTTTCATTCTTTCAACTACTTCTTTATAAGCAGCAAATTGTTCTTCTTCTGTTGGTCAGTGTTCATTATCCATGTATAAGAATTCTGAACGGAATAACCCAATTGCTTCAGCATCATTGTCAATAACTGCATCAACATCTTTTGGACGTCCAATATTTGCAGCTAATTCAACATGATGTTTATCTTTAGTAATTGATTTTTTACCTTTGTATTTAGCAATTGTTTCTAAATATTTAATGTATTTAGCTTCTTCTTTTTTATAGTGTTCTGATTCTTCTTTTGTAGGATCAACAATTACTTCGCCTTTGATTCCATCAATAATAATTGTTTGACCATTTTTAACTGATTCTAAAACATTGTTTGTTCCAACTACTGAAGGAATGTTCATGCTTCTAGCCATAATTGCTGTATGACTTGTTGGTCCACCAATGTTGGTTGCAAAACCTTTAACATATTTTTTATTTAATTGAACAGTTTGAGAAGGACTTAAATCATAAGCCACAATAATTACTTCTTTATCAATAGCAGTTAAATCAGGTTCTTCAATGTCATTTAAAATGTAAAGAAGTTTTTTCATAACATCTTTAACATCAGCAGCTCTTTCTTTCATGTAGGCATCGTCCATATTTGAAAACATTGTTGCCATTTCTTCATAGAAAGCTTTTGTAGCATATTCTGCTGTTTCTTTATTGTCTTTGATTCTACCTGTAATTGTTTCTAAAGCTCAAGGGTCATTAATAATTTGTAAATGCGCATCAAAAATAGCAGCTTGTTCAGGAGTTTTAGCTAATTTTTTAGTAGCTTCAACTTTTTCACCAGCTTTTTTAACAGCATCATTATATAGTTTTAATTCATGATCTGCATCCTTAGCTTTTTCAGTAATATTGACTGGTAGTTCTTCAATTTTAAAAACTTTTGCAACCGCGATTCCACGACTTGCTCCGATTCCTTTAATTGTCATTTTGACCTCCATTTTATTTAAATATGTGTGTAATTTAATTATATAAACATAAAGTCTTTTATGGCTTTTTAACTATCTTAATAAAAATTAAATAGGTAATCATTTCCACAAGCAAAACACATTTTTTTTAAAAGCATTTTACATTTTAAATTTTTCATAAAAGCCTGTTTTATAGTTTTTTTAGTTTAAATTGTTAATTTTGAAAAAGTGAAAAATAAAAATCCGCTTACGCGAATTATTATCAGATTTTGACTCTTTTTTCAGGTGCTAAATACATCTTATCACTAGGTTTTGTTTTAAATGTTACATGAAATTCATCTAAGTTTTTAGCTTGAATATTTGCTCTTAAATCCTTAGGCGCATGAACATCAGCAGCTAAAAGCATTTTAGCATATTCAGGTTTTTGTTTAGCTCTTCAAACTGTAGCTCAGTTTGTAAAGAATTCTTTTGATGAATGATTTAATTCTTTTTGACTAGCTTCTAATGCACATCTAATTCCACCAGCATCAGCAATATTTTCAGATACAGTTAATTTACCATTACATTTACCAAATTCTGAATCTTGGCCTTCAAAAAGATCAACCATAGCTTGTGAAAGCTTATTGAATTTTTTGAAATCTTCATCTTTTCATCATGAATTTAAATTACCTTTTTCATCAAATTTAGCTCCATTATTATCAAAAGCATGTGAAATTTCGTGAGCAATAACAGCTCCAATTCCACCATAGTTAGCTGAAGAACTTTGGGTTAATGAGTAGAATGGTTTTGCTAAAATCCCAGCAGGGAAAACTATATGATTCATAAAAGGATTATAGTAAGCATTCACTTCAGCAGGTGACATACCTCATAAATTTTTATTAATTGGTTGTTTGTATTGACTAAAGTTATATTCGCTTTCAATTGCATTAAGATTTAATGCATTAACTACTAAATTACCTTCATGTTTATATGGAGTTATAACCATTAATTCATAAAAAGGTCTAAGTTCACTAGGATAGCCAACATGAACACCTAAAGCATTTAATTTAACAATGGCTTTATCTTTAGTTTCTTGTGAAAGTCAGCTGTTGTTTCTTAAACGGTCTTGATAAATTTTGATCATTTTTTGAACCATATATTCAACATTTCTTTTAGCTTCAGGACCAAAATTTTCAAGTGCAAATCTTAAACCAAAAGGAATTTTAAAGTATGAATAAGCTAAGTAGAAAGCACTTTTAGCTGCACTTGAAGCTTTATCAATTCCACTTACTGCACGGTTAAATTCTCCTGCAGTAATTCTAGTTTTATTGTCCAAAAGATTTGAAAATCTAATTAAGTTTCTAAGATACATTCAGCATTTAAATGTTTCAAATGTTCTTGAGTTATATAAGCTATTTAAACCTTTCATAAATTTAGGATAAATTACAACAACTTTTTCGCTAACTTCTTGTTTAATTAATCTTTCTGCTATTTCTTTAAGATCAAAAACTTTTGAATGTTCATTAATTTCATCAATTGTAAAAGGATTATATAAAGAGGGATAGTCAGCACTTTCAACTGCACTCATTGTGTATTTAACTAATGAGTTATCAAATTCTAATGTATTAGAAATAATATCTTCAATTTTATTTTTATCTTTAAAATAAGCAGCTAATAATTTTCTTGACATGTTTTTAAATACTTCTAAAAGCGGTTGTTTAGCTGGATGATTGTCATCATAATATGATTTTTCTGGCAAAATTAAACTTGGTCCACTAAAACCTAAAGTTTGTAATTCGGTATTTTTAAAATCTTGCATTACTTCAAAATCAAAAGGCAAGTGGAATTTTCTTAATTGATAAAAATCAAAGTTTTCAATTAAATCATCTCATGATTGCATTTCTCAAATTTTATTTAAGTAAGGTTTAAGTGGTTTAACTTTAGATTTATTTCTAAAAGTAAAATCACTAGCCATTGAATAAAGATTTGTGAAGTTTAAAATTGCTCCAACTTCTAATTCACCTGCATCACGAGTTCTAACTAAATCACTAGCCATCTTTCTTAAAATTCTTTCATTGCGGTTGTGAATTTCTAAAAATGCTCCTGTAGCACTTTTATCTCCAGGAATTTTTGCTTTTTCTAATCATTTTTGATTGACTGCAAAATATAAATCATCTTTAATGTTATTTTTTTTCATTAGTTTCTCCATTTCTATATCTAATATTATATTTAATAAAAGATTTTAATTAATATATTTGCTTTATTATTTTTCTTTGATATTAGCTAGCATATACAAACAAGCTGTTTCAGCACGTAAGATTGTTTTGCCTAATGAAGTAATTTTAATATTGTTTTCTTTAGCTAATTCTATTTCTTCAGGACTAAAACCACCTTCAGGTCCAACTATTAAAATTGAACTAGTTGGATAAGTGATAATGTTATTTTTTTGGTCTTGATTTTCATAAGCTAAGTACTTTTGCATAGTAGGATAAGTCTTAATAATTTCTTCTAAATTCATAATTTTAGTTAAATAAGGAATGATGTTTCTAAAGCTTTGTTCAGCTGCATTTTTAATGATTTCATTAAACCTATTTTGCTTACTGTTGAATTCATATTTTAAGATTTTTGCATCAGTATATTTAGAGGCAAAAGGAATAATTGTAGTAACTCCTAATTCAGTAGCTTTTTGCAAAATTCATTCGAATCTTTTAATTTTAATAATTGGTGCAGCAAGAATAATTGGCTTATTAAATTCATGATTTATATCTAATTTTTCGATAATTAAAGCTTTATTATTTTCTAATTTGCACTTATAAAAAATGTCTTGATAGTTACATAAAAATTCATCTTTTTCAACACGTGCAACTTTGATGTGATTTAATATCTCTTTTGATAGTTCAAAATGATCTTGATCACTTTTTTTAGTAACAAAAAATCTATGCATTTTATTCCTTTCATTTAACTAAAACAACTGTTTTAAAGCAATTTAAGTAAAATTATATTTTTAATTTTTAAATTAATTTATATTAATATTTTAGCAAAAAAATAAAAAGAGGATTAAGATAACCCCCTTCTTTTCTATTAATTTATAAAATACAACTTTAACTATTAATGATTTTCATCAAAGAAATTTGGCATTACATCTTCATTTTCATCTTCATAGTTTTGAGCAGTATAACTCATTATACGAGTATGATGTGATCCATTAATTTGTGTATATGTATTTTGCATATTTTGCTCATAATGTTCACTAACATTTCTTTGCAATAATTTTGGTACCGCATAAGTATTTGTTGAAACATTTGCATTAAGATTTGAAGCAATAATACTAATTCTGAATATTTCTTCACGATCTTGAGTATCGATATATTGAAGTCCGATAAAAGTTTGAGCTTCAAGGTTATTTGCTAAATACTCATCAATTCGATTTTTTGCTGTTTCAATGTCATCTAAACTTGCTCTTTTATCACACTGAAATAAAATAGCAACTTTTTGACAATTTTTAATTTCACTTTGAAAAAGTGGAGTCATTAAAGCATCATCAACTGCTTTAATAGCTCGGTCTTTGCCAAAGCCATTACCAATTCCTATAATTGTTTCTTTTCCATCTAATAAAACTTGACGTAAATCATTGAAATCTAAATTAATAAACCCAGTTTCAAAGATAATATCACGAATTATTTTGATTGAATTTTTCAATGTATAGTTAGAAACTTTAAAAGCTTCTTGAACTGGTAAGTTTCTGTAATTTTCAACCAATTTTTGGTTTGAAATTACAATATAACTATCAACAACTTCACTTAATTTTTTCAAGCCTTCTTGAGCAATTAAGCTTTTATGTTTTCCTTCAATTAATTCAAAAGGAGTGGTTACTACTGCCACTGTCAAAATGCCCATCTTTTTGGCAGCTTCGGCAATCACTGGTGCTGCTCCAGTTCCTGTTCCGCCGCCTAAGCCAGCTGAAATAATAACAATATCTGTGTTTTCTAATGATTTTTGAATTTCTTTAATGCTTTCTAGTGCGCACTCACGTCCTACTTTAGGGTCCCCACCAGCACCAAAGCCTCTGGTAGAATCACCTAAAAGTATTTTATTTGGACATAAGCTTTTAACCAAATCTTGACGATCTGAGTTAGCAACTAGTAATTCAACATTTGGCAAGTTTTCATCAAGCATCAAGTTAATGGCGTTATTACCTGCACCACCAACACCAATGACTTTTAGTTTAACTTCCAAATCTTCAGCATTTAAGTCCATAGGCAACCTCCTTATATTGTTTTATTTAATTTTGTCTTATTTTTGAGCTCTTTGTGATGGCAATATTCATGAGAATAATTTGTTTCAAAAACTTTTTGGTTTGTATTCTGGTAAATTATTCAAAGTATTAACCATGTCTTCACAATTGATTTTTTCAGGATTTAGTAAATTAATAATGCCTAAAAGGCTATTAGGTATATTGAATAAAGTTGAGACGTTATCGTCTAGAATTGCAACATTCATTTCGGGATATAATTTGTTAAAACGATCCATAAAGATTTCTGAGTTTTTGCAGTTTAAAACCAACTCATCAACTTTTTGATTATTTTGGTCTAAAAACGCTTTAACTTTATGAATAGCTTGGTCAAATAAAGAAATCACTAGTCTATAAGCTTTATAGCCTTCAGTTTCATCATTTAAAACTTGGTAAACTTTTCAATTAGCTACCACAGCTTCAAGTAACAAAATTGTATCTTCTTTACTTTTATTTAATTCTTTTGATAAATAATTAACAAAGTTTTTAAAGTCAATGTTAGATTTTTCATAGAAAAATGTTTTAGCATTCATAGAGCCATTTACTACTAGTGCATTTCAGTCAAAGTGGAATAAGTATTTAGCTTTATCTTTTTCTTCTAAATTTGCACTAAGAGCTTGAGATTCTAAAAGTAAAGCATCAAGTTTCAAATTACATTCTTTAATAATGTTAATAATTTTTGCAAGTCAAGTATCTTTTGTAATAACAAAAAGTCCTTGATTAATATTTAATTTTAGAGCTTGTTTGTTTCAAGGAAAAGTACTGTAGCTTTTAACAACATCATTGTTAATTGTTTCATATAAATATATGTTTGAATTAATCAATTCGTATGTTGAACTTTCTTTTTGTTCTTCAAGTTTATTTTTAACTTCTTTTCATACTTGTTGATCAACTGTATTACTTGGAACATCAAAAGTCAAAGTTTGGTTAACAAATTTTAAACTTTTGTATAAACTATCTTTAACTATAAAAGAATATTTAACTACATCATTTTTCTTGGTAGCAACTTTAGATTTCAAATATTTTTTGAAATTCGTAATAAATTCTTTAATCTTATTCGCTTCAAAGTTTTTGGTTTCTAGATATGTTTGAAAAACTGGAAAATATTTAGAATTAATATCTTCCACTACTGTCACATCAAGTCTAGTTTTTTTAAGAAAGAAATTTACATAAATTTTTTTCATAATTACCTCCAAAATAGTCTTTTTAGCAAAAAAAGATAGATTAAAAAAATTAAAAGCATTTTTTCAAAACTCTTAACTTGGCGCTACGAGAGCGTTTATTAAGTTCTAACTCATTATTGGTTGGAAGAAATACTTTTACTTTATATTGCTTCTCTTCATTAATTGGCATTTTTGCTGGTAATTTACTTTTAATTAGTGAACCAAAAAAATGTTTAACAATGCTGTCTTCCAATGAATGAAAAGTTATAACGGCTAAAGAAGTATTAGGTTTTAATAGTTCAATAGCATCTTGTAACATTTGCTTAAGGGAATCAAATTCATTATTAACTTCAATACGGATAGCTTGGAACACTGCTTTATTAGGATTTTTTAGTTTCACTAATTTTGCAGGATAAGCATCTCGCACTACTTGAGCTAATTCCAAAGTGGTAGTTATTGGCCGCTTACTAATGATGGCCTTCGCAACCTGACGAGCAAGTTTTACCTCAGCGTAGTTTCTTAATAATTGTTCCAATTGACTTTCACTATAATTATTAACTACATAGTAGGCATCAAGCTTTTGACTTTGATCCATGCGCATGTCAAGACGTGCATTTTTGTTATAAGAGAAACCTCGTTCATCATTATCAACTTGTGGACTGCTAATACCTAAATCAGCAATGATTCCATCTACTTGGCTAATGTTCAATTTATCTAATTCACTTTTTATATTTTTGAAGTCAGAATGAATTAGTTGAAAATTAGTTGATTTTATTTGGCTGAGACGTTTGCGACTTTCTTCTATCGCAAAAGTGTCCTTATCAAAAGCAATCAAACGACCAGTGTCAAGTCTCTTTAAAATCTCGCTAGAATGTCCACCCATGCCCAAAGTTAAATCAACATAAATACCATCTGGTTTTATATTAAGGGCATCAATAGACTCTTGCAGTAAAACAGAAAAGTGTTGATTCTCCATTTTATTTACCACTTCTTTCAAGAATTTTTTGTGCTGCTGAAGATATGTCACTGTCATTAAAGTTTTGACAATATTGATCAAATTTTTCGCTGCCTCAAAGTTCTACAAGATTACCTGAGGCTACAAAAATGGCTTCTTTTTGGATAGCAGCTTTGTCGAGGAATTGTTTTGGAACAACGAAACGTCCTTGGTTGTCAAGTTCAATTTCTTGAGTATTGCTCAATCAAAATCTTCTAACAAAACGAGCATTTGTATCAAAAGAGTTTTGTTGATTTAGAACTTCAACGAATTTATTGAATTCTTTTTCGCTTCTAATTTCAACAATGCCTTCAAGTCCGACAGTTAAATAAATTTTGCTTCCTAAATCGTCACGCAATTTAGATGGTAAAGCTAATCTTTTTTTGTCGTCAATGTTTCTTTTGTATTGTCCAAACATTTTCCACTTCCTTCCACTATCCACCACTATTTTACACTATATTAAATACATGTGCAAGAAAATATTTGAATTTTTTCATTAATTTAAACAGCTTTTGCAAGCAACTTTTGAAGGGGCTATAACTAAAAAATGCGTTTTTCAGCGAATGTTCGCTGAAAAACGCATTTATCGAGTATAAAACCTTCAAAAATTTTGTGAAAATTAGGCAAAAAATTATTTTTTAGAATATGTGGAGGACTTTCCCTAGTCTTCGACATTTAAGAAACCAAAAGCATTGGTTACATCTTCAACCTTACCTAGAATTGTAATAAGATCACTTTTTTGTAAAACAGTTACCCCAGTAGGTAAAATACTTTCAGTTCCTCTCTTAATTAAAACAACTGAAATTCCTAAGTTAAAGAATTTTAATTCTTTGATTGGTTTATTGATTAATTTTGGATTATTTAAAATAGTAGTACCCATAACGAAGCCATCGCCAATTTCTTGTAATGTTTGAGAATAACGGCTAAAATTTGGATTAGCTGCTAAAAGAGCAGTACGAACACCGGCTTCATATTCAGATCTAACAATAACATTAACTCCAATTTGTTTTAATACACGTGCATGTCTTTTACTTGTTGCACGAGCAATAATATTATTAACTTTTAATTCGAGTAAAGCAGCCACAATTTCAATATTATCAGGAACAGCAACAACAACAGTTCCAATTTCTTGAAGATTAAGTGCTCTTAAAGCTTTCATATCACCTGCATCAGCAATAACAACTTTTTGTGCTAAATCAGCATATGATTTTAAAACTTCTTCATCTTTATCTATTAATAATAAAGGACAATTCATTTTAGCAAGTTGGCTTACAACCACACTACCAAAACGCCCTGTTCCAATAACACAGATATCTTCATTTTTTCTTTTACGAGGCATAATTTACTCCTTTGTGTTTTTATTAACTTATGCTATAAATTATAAAAGATTTTTATTTTTTTATAACTAATATTTTAACTCCAAAAATTTGGCAAACTTTTTTAAATAAAAAAGAAAATAGTGCTCTATTTATTAATAATAAATAAATTTCATAATTTTAAAATTAAGTTAAAATATATGTTATGAAAAATTCAAAAATTGCCGCTTGATGAAGAAAAAGCAAAATTAGACAATTTTTTGCAAACTATAGAGTTTGAAGATTAAATATCACCAAAGTTAAATACATCTTTTTAGCTTATCTTCTTACTGTTATTATTGCAACACTTTTCCTTTTAAGTCCTTGAACTCATAAAGATAGTAGTGTTAAAGTTAGTTTTTGAGATGCTCTTTTTACAACATCTAGTGCTTTCAGTGATACAGGCTTAGTCACTAAGACAACCTATAATACTTGAAATATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGAATAATTTTTAGTTTTGGTTTAACTTTCTATTTCTATTATAATGAACCAAAAGCTTATAGCGATTTTGATAAATATGGAGATTATATTTCACCTTATAAAAATTGGGGCCTAAGTTTTAGATATGGTTTCTTCCATTGTATTAGTGCCTTAAATAATGCAGGCTTTGACATTATTGGTAAAAATTCACTTATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGAAGACTATTTACAAAAATTAGTTTATTAACTTACTTTGTCACAACTTTAGTAGCCTTTATCTTAATTATTTCCTTTGAAGGTACTTTTAAAAATGATAATTATTTTTGAAGAAGTGATGATAAGAATTTAGGCAATAAATGAGAAAAAGCTTGAGCATTATTATTTACAACTTTTTCAACTAGATCAGCAGGATTTTCAACTATTAATTTTAGTAAACTAACCTCTACTTCATTAATCATTTGTTCAATTTTAATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGAGGTAAAAAGAAAAATTATTCTAACAAATATGAATATATTTCAGAGCCTGTAGCAATTGGATAAAATTATGCAAAATAATAATTTATTAGATTTAGGAATAAAAACAGAAAAACTTGAAAGATGAGCAAGCTATAGCACAAATAAAAAATATAGAATATTAGTTTCAGTTTTTTCAACCTTTTTATTACTAACAATAGTTTTATGTTTAATCTTTATTTTTATCTTTAAACATGAAACTAAAGTCTTAATTTCTTTAAGTATTGTTGCTTCAATAGCTTTAATAATTTGATTCTTATTTTTAGCTCCTTTTACTTACTTAATGATCACTTCATTTTGAACCTATAGAGCTATAAAACAGCCAGATAAACCAATTTATAGAAATTATAAAGAAGCTAATTGGTGAATTAAAATACAATTAAATTATGCTAATTTTGGCTTTAAAATATTTAACAAGAAAGCTTTGCATTTAACTAAAGAAGAATACAAGCTTTTTGTCAATTTTTACATGAATGTAAAATAATAGTTTTTATATTTTGCATTCTTTTTTATATAATCTAATTATTATGAAATTAAATTTATTTGCGTCTGAATACAAATCATTGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGATTAAAGATGATTAATGAATGAAATAATTTAAAAAAACACGATTAGCATAATCTTACTAACGTGTTTTTTTATCATTATTTTTTATTGCTTAAATAGTCAACAAATTGTTTTGTTATAAAAGTTGGTCTAGTAATTGCTGAGCCAACAACTACACCATTTGCACCTATTTTATAGGCTTTTTGGACCATTTCAGGTGTATTAAATCCGCCTTCAGCAATTATGTATTTTTTGTACTTTTTATTTAATTCAACTACTTCTTTTAGAAATTCAAAATCATTGTAACTATTAGGTTTATTTAATGTGTCTTTTGTGTAGCCTCTAAGAGTAGTTGAAACAATATCAAAATCTAATTTAAAAGCATTTTCAACATCTTCAATTGAAGAGCAATCTGCCATTAATAACTGATTATGATTATTTTTCTTGAAATATTGAACTAAGTCTTCCAAACTTTCTTTTGGTCTTTTTCTTAAAGTTGCATCTATTGCAATTATTGGACATTTACTTTCAATTAAGAATTTTAAATCATCTAAATCAGGAGTAATAAATACTTCAGAATCTTTAGACATTTTTTTAGTAATACCAATAACAGGAATGTCTTTAGGTACTATTCTTAAGACTGGTTCAATATTTTCTCTTTGGCTAATTCTTATGCCTTGAGCACCACCTAATAAAACAGACTTAACCATTCTTTCGATTACACCAGGACCATACATTGGCTCATTTTCCAATGCTTGACAAGAAACTATAAACAAATTATTTTTAAGACTCATCACTTAATTTTTCGTTAATTTTACTTGCAACTACAGTTGCTTTTCCGCCAAGTATTACTTGAACTGATAAATTATCATGAACTAAACCAAATGATCCATATGGTACAAAATCTTCTGCTTTAATATCAACATTTTCTTTTAATTCAATTCTTAATCTAGTTGCACAAGCAGAAACATTGTTTAAGTTTTTAAGACCGCCTAATTTATTAACAATACCTTCAATATTAGCATCGTCAAATACTTTTTCTCCGCCTGCTTGTTCAATTAATTTTCTATCAGCTTCTTGTTGTTTTAATTCTTGATATTGTTTTTTATTAATTAAACCAATATTATTTCCAAGACGACCTGGAGTTGCGATTTTGCCATATTTAATTCAGAAGAAGAATGTTACAAAGGCAACACCTCCTAAAACAAGAGCTCATAAGAATGCAAAGTAGAATGCAGTACCTTTAGCAACTGGAATAACACCATAAATAATTAAGTCAATGAAACCACGAGCAAAACCAACACCAACGTGAGCTCCTGTTCATTCCATTAATGCATATGCTAAACCACTAAGTGGTACATAAATAGCATAATAAAGTTGAGGAGCAATAAACAAGAATGTAAATTCAAGAGGTTCTGTAATACCTGTTAAGAATGCAACACCCATTGCTGAACCTATTGTTATCGCTACATTTTTACGATTTTCTTTTTCAGCTAAAAGAATCATAGCTAAACCAATACCCATACATGTTCCAATGTAGGTTGGATAGTCTTGGGTAAATCTTCCGGCATTAACTCCAAGAGTTTTTTGGAATCAAGGGAATAATTTAATATTATCAATGCTATTGTAATTTAATTGATTAATGTAGTTTCAGATGTTTTGGTCACCAGTAATAACATCTTTTCCTTTAGAAAAACTCTCTAATGAATTTTTTAAATCAGTAGCTACTTTTGAAGAAATTTTACCAGCTGTTACAGCCGCATCAATCATTTCCTTAGATAACACACCACCAAATTCAGTTTGATATGCTAAAACAATTGGAATGTGGTGTAAACCAAAAGGCATTAAAGCACGGCCTAATATACCATAAATAAATCCATCTAAACCTATTGGTGATCTTTGCATTAGTTTACCTAATTCATAAATTGCTAAACCAATTCATGGTCATAATAATAAGAATACAATAGATACACCAAAAGAAATAGGTACTAAAATAATTGGCACTAATCTAATTCCTGAGAAGAATGAAAGTGCAGGTGGTAATTCAATTTTAGAGAAATAATTAAATGTATATGCAGTGACAATTCCAATAACCATACCACCAAAAATACTTGTATTTAATGATTTCATTCCTAAGTTACTTCCTACAAGTCCAGCAACTGGTTTGTGGAATCAAAAAATACTTTTAACAATATCAACACCATCAAATTGGATGAATGCTAATTGAGAACTAGTAAAGACAAGGTAAGCAATAGCAGCCGAGAATCCTGCTCCACCTTTGTCTTTTGAAAATGTGATCGCTATGGCAATACAGAATAAAATAGGTAAGTTTCCAAAAATAACTTCAGATACACCTTTGAAAATATTAGCAGTTGTTTGACCAGCACTATTAGTTGTATTTGCACCAATAGCTCCACCGATTCCTAATAATAAACCAGCAATAGGTAAAATTGCAATAGGCAACATTAAACCTCTAGCTAGTTGTTGAAATCAAGCTTTGATTTTTCCTTTTTTAGGATTGCTCTTAATTTCTTTATTAGGAATTTTAGCTTGAGCTTTATCCATAAGCTAACCTCCATATTATTGAAAAATGAATTTTTTTCACTTTTCACCATAATTATATTTAGATATAAATATAAGAATATTAATTTGTAAAATCCTTACAAAAGAATACCAAAATTTTGTATATTTTTTTCACCCTAAAAGACTAATTTAAGGAAATTTAAGACATTTTTTACACATATTTTGCATTCTTTTTTTATTAATATAAAATTAGATAAGACAAGGAAGTACAACATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGATTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGATCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAAACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATATTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAAAAAACGATAAAAATTGAAAATAAGAAGCAACAAAAAACAAGAAAAAAATATTTTTGTAAAAGTCTTGTTTTTTTGTTTTTTTATTATAAAATAATAATGAATTTTTATAAATTCAAATTTGATCAAATTTAAATTAAACACACTATTATGGATTCACTTGGGTGTGCCTGGAGAAAGGGTGCTAGGTGTTAGAAATAATAGGAAGTATAACAAACTAAAGGAAAATTTTATATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGAAATCCTCGTATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAACAAATAGCTACTATGTTACTGAAAGATGACTTGGTGGTACATTAACTAATTCTTCAACAATTTTCAAAAGAGTTAAAACTATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAGGAGTTACTATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGAGACATTGAAAAAGCTATTACATGATTAAAAAGTAATGGAAAAATTAAAGCTGCTAAAAAAGCTAACAGAGTTTCAGCTGAAGGTTTAGTTGCTATTGCAGCTAATGCTACTAAAGCTGTTATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAACTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAATTATTTTTTAAAGTTACCATTTGACTTTTCAAATGGTACTTTTTTTGTTTTTAAAAATTATTTTAAAATCAAAAAAGTTATACACAAACAATCAACATAAAAATTGATATTGGATTTTGACCAATTAAACTTATTTAAAGGTCAAAAAGTAAAATAGACAAAAACTTATGCACAATTTTCAAATTAATACTTTTTTGGAAAAATGGAATCATTTTCTTTTATACAATTAAATCATGGAAACAAAAATAACGACGAGTAACGAACAGTTACCAATATTGAAACCTATTTTATTAGCATCAGATTTCGTCGAAAAGATCAAAGGAGATAAGTTATATTCAGATGTGATGATGCTAATAGCACAAGATGAGGATTATGTCTATTTTGTCTCATGTTTAAAAGAATGAGAAAAGAATTATGAATTGGATCCTCGTTTTATTAAAATATATGAGGAGGAAGGCATAATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAATTTTTCTATACACTACGATATTTTGAATATCGCTTTTGGTATTGGGCGATATTTTTTTGTAAAAAAATGAGCCTAATTCAGACTCATAATTCTATTATTCATATTGGTGCATATCAACATTAAATTTACTCATTACTTCATAAATTTCATCAGCATTTTTGGCTTTCATAATTTTATTATCCAAAGTATTATCTAAAGCTAAAATACTAATCATTTGTAAAGTATCAATTTGTAAATTACGATTATTTTTACTTAATACTAAAGCAAAAACTAAATTAACATTCTTTTCATCATCAGCTTTTCAATCATTAACCCCATCATTATTCATAGTTAAAATACATAATGTGTCTTTTTTAACTGTTGAGCTTAATGCTTGACAAATTGCAATGCCACCGCCTATTGCAGTATTCATTAATTCTTCTCTATATAAAAAAGCTTCATATATTTTCTTTTCATCACTAACAAAGTTTTGTTCATTTAGTTGATGTGATAAAAAGTGCAAAACATCATCTTTTTTATCAAAATTTGAATGTACAAAAATAGAATTTTTGTTTAAAAAATTATTGAATTTCATTTTAGTTGTTCTTTCTATTATTAAATTTCTCTTTTTTCTAATCTAGGCATGATACTTAAAGAGAAAGCTTCAGGACCTGTATGAACTGCGATGGCAGAAGATGTTCATCTTTCTGTATCTATTTTAATATTTTTTTCTTTTGAAACTTTACGAACAGTTTTATTAACTTCTTCATCAATTCCGTGAATTCAGTCAATTTTGAAGTTTTCTGTTAATGTTTTGAGATCTTTTGCTTTAACAAAAGCTCCTAATTTTTTAAATATTTGAATTATTGCACCCTTAACACCACGAGCTATTCCAGCTGTTGAAACATGTTGTTTAATAAACTTAACATAAGGATAAATTCTTAATTTAGACATTGTTTTTAAAACGAATTTTTTAAAGCTATTATTAACTCTACCGCCCTCAATCAAATAATTAAGTTTTATTGGCAAAACGTAAATTATTGAATTGTTTTTTATTCATTCTAATTCTTCAAATACTTTGTCTAGTGTTTGATATTTTTCATAAACTTTTTTAATATGATTAATAATTTCATTGTATTGGTCACCAACTCAGCAGTTATCATAAACGTGAAAGTTTTTATATTCTTTGCAAATTGTTGCAATGTTGTTGCAACTTGAAGATAATGATGAACCAATTGGGAAGTAAATTACTTCATCATATTCTTTAGTCATTTTGTCCACTGTATCTAAAACAGCTTTAACATTTGGCATAGATGATTTAACTGATTTTCCTTGATCAAGTTTATGCAATAAATCTTCTTGGTCTAAGTCAATACCATCTTTATAAACTACACCATCAATATCTGTTAAAAAAGGAAAATAACCATAACCTTTTTTAAGAATTAGTTCTTGCGGTTCACTAATAAAGCCGTCAACAATAAAACCTATTTTTTTCATTATTGACCTCCAGTCTTTAAATAAAAATACTTATCTATTATAATATACAATTATAAAATTTTATAAATAATTTTAATATCATTATATCATTAATTTTTATGCATTTTTATTTTAATTTTTTAACATTTTTAATTTAGACTAAAATGCCTATAAAACAATAAAAAAAGAAAAAACACATGGTTCATCTTTTTTCTATTTGCAAACATATTGTACATCTAATGTTTTTACCATAAGTTCATCTAAGCTTTCTGGTAAGTTTTGATAAATAATAACATGATTTTTAATAGTGCTAATTGTTTTAACGAAGTTTAAAAGTTTTATGTATAAAATTTGATCTTTTTCAATGTATTTGCAAATCTTTTCCGATATAACAAAAACCTTGATATTTCCGATATTAACATAGTTAGTTAAACGGTCATTTATCTTTTCGATATATAAACCTAAGTTAATGCCTTCTTTCAAATTACTAAAAGCATTGTTAATTAATCGATAATTAAATCCGTTTTGTAATGGTTTAATTAATAAAGTTGTTTTTGGTCGATCATATGTATTTAAATTAATTAAGTCATATTCATTTGTTTCCAAAATAATGTTTTCATCGTCTTCACAATTTTCCATTAAATATTTGTTTCATTTATTGGTATAAATCAAACGATATCACGAAACATTATTAAAGAATTCTGAATCTTGGTCAGAGTAACCTATGACTCTATTTTTTAAAATTGCAACTTTGCTTTGACGTTTAGTTTTATATGAAATAATTGGAATGTTTTCTTTTATATATTGTTGCAATTCATTTTTCTTTTCATAATCAAGCATCATAGATTTAAATTCTTGATAATGGTTGTCATGTTTTGTATCTGAAATTAATCAAGAAGAATATTTATCAAATTTGCGTTTGTTATTTAAGTGTTGTATTAAGTATTTTGATTTATCAACTAATTCTTGAACATCATCAACACTTTTGAAAGTGTTAACACTTAAAAAAGCAAAGCTATTGAAAAATGGATTAATAATTTTTCTTTCATGTAAGAATTTTAATCTTTTAAGTGCTTTTTTAGTGTAGTGGTTTAGCCAGAATGACGAATTGGTTTCAAAGATTAATAATATTAATCCGTTTTTAACGTAGTAATATCCAAACATATTATTAAGCTCAAATATTCTCAAAATATCTTCAATGTCTGATTTACTAATGACATTATTGTAGAGAAATTCATTGATGCCCAAAGCATAAGCCAAAATATATTTTTCCTTATTTAAAGCATTATATAAATCTTCAGATTTTTTAATAAATTTAAATGTTGTTGAAGCACTTCTTTTTGGTCTTGCGTTTCAGTGGATTGTTAAGTAGTATCGATTATTTCCAAGAGGGAAGAATTTTAAAAGAATTTTTTCTTTAGTCATTTTAATGTTTAGAAGTTTTAAACCTTGAGCATTTTGATGTGTTCGATAGCCTTTAGAACTCATCTTGCAGTAAATATTAAATCTTTTATCATTATTGTCGTTTTGTGAAAGATATTCAGTCAAGTTTTTTTGGCTCTCTTCATCTAAGTAAGAAAGAAAGACATCAAAGTCGACATATTGATTAGCTGAAAGACCTTGAAGACGTGAGTCCATGTTTAAAGATCCACTTAAGCTTTTAGAACTCAAGCGAATTATTTTTTTTTGTCCAAAGTCTAATTTAAAGATTGCTAAACCATTTGTTTTGACTTTATAAACATGATTTAAGTAAATAAAAACTATAATAAAGATGTCTATTAAAATAACACATGCTATTATAATTGCTATTCCCAATAATAATTGATCCATAATGCTCCTTTTTACTAAAATAGCTGTTTTATAGGTTTATTTGTTAATCATCTGTATTTTTTAAAAGTCGAATTGAAGCGTGATTTTTAGCTAAAACTTTGATTGTTTTTTGTCCTGTGAATCTAACGCTAATTGTATCGTTTTCAACAGCCACAACTTGGCCTTCGCCAAAGAAGGTGTGGCTAATAATGTCACCTACTATAATTTTTTTGTTCAAAGCTTTTATTTGTTTTTCGTCAACTATTTCATCAAAATCAAGGCCAACAGAACCTGTTTTAAGAATAAAGTTTTTAAGTTCAATTCCCATTTCACGAATAAAACGTGATGGTTTCTTTTCTAAATCTGTACCAATTAAATATCCACGACTATCGCTAATAAATAATCTATTTCTTGCTCTGGTAATTGCCACATAAGCAAGTCTTCTTTCTTCTTCTAAAGCATCATCTGAGTTTGATTTTAATGATTTATAGTTAGGGAAAATTTGATCACTCATTCCCACTAAAAAGATATTGTCAAATTCTAATCCTTTAGCAGAGTGGACTGTCATAAGTGTTACATAATTTGTAAAGTTATCATATTCATCACCTGCTGAAAGTAGTGAAACTGATTCTAAGTATTCTTTAATACTCTTATTTGGATTCTTCTTTTCTCAAGTTACTATTGAGCGAATTAATTCAGCAACATTATCTTTAGCAGTGCCACGAAGTGAGGTTTCTTCTTCTATATGTTCATAGTAGCCAATTTCCTTTAAGAATTTATCTAAAACAACACTAATTGAGTTGCTTTTTAAAGCTTGACGATAAAGGTTGATTTTGTTAAGAAACTTAACAATGTTGTTTTTAACATTTAAAGGTACTGGCAATTCCTTGTAATATTTAATTAAATATGAATAGAGAGTTAATTTTTTATTATTTGCTGCTTCTTGTAATTTTTTAAGAGTTACTTCTCCGATTTTACGGTTTGGAACATTAATTATTCTATTTAATGAAACATCAGAGCCATCATAGATAACTCTTAAAAATGCTAGAGCATTCTTAACTTCAGTTCTTTGGAAGAATTTTTGACCATTAAAAATCTTGTGGTTAATATTTTCATTAATTAATTCTTCTTCGAAAGGACGCGAATAATAATTTGAACGGTAAAAAATAGCAATATTTTTAAGCTGAATTTTTTGTTTCTTTAATTCATTAATTTTTTGGACCACTCATCTAGCTTCAGCTTCGACACTAAAAGCATGAATAAATTCTATTGGTGCTCCTTGTTCACGATTTGTTATTAGATCTTTACTAAAACGTTTTTTGTTGTGTTTGATTAATTTGTTAGCTGCATCTAAAATTGTTTTAGTTGAGCGGTAATTTGTATTTAAAATAATCGTTTTAGTATTCTTGAAATCCTTATCAAAATCAAGGATCAAATTAACATCAGCTCCTCTTCAACCATAAATAGTTTGGTCAGGATCACCAACAATTGTTAATTGAGCATTTGAAGATAAGTATTTAACAATATCATATTGGATAGTTGAAGTGTCTTGGAATTCATCAACTAAAACATATGAATATTTTTTACTTCAAATTTTTGCCACTTCAGGGAAGTTGTCAAATAATTCCTTAACTTTGATAATTAAGTCATCAAAATCGAGACATTTTTTGCTTGTTAAATATTGATTATATTTAGAATAAATTTTTCCAACGAGTGCATTAGATCTATGCTTTTCTTTAGCATCTAATTCTTTAACAATTTCGTCTTCACTAAAGCCTTGGTTCTTGGCTCATGAGAAAATCTTCATAACATTTTTGTATGAAACATCTTCATTTGAAATGCCTTCAGCTTTATAAAGTTGAGCAATAATTGTCTTCTTATCTGTTTCATCAATAATTTGGAAGTCATTACTTAAATTAACATGAATAGCTTCCTTCCTTAAAATCTTTGAGCTTAAAGAGTGGAAAGTACAAATATCGATTTGATTAGCTTCTTCGCCGACGTATTGTTTAACACGTTCGCTCATTTCGTTCGCAGCTTTATTAGTGAAAGTCACTGCTAAAATTGAACGTGGGCTAATTCCAAGTTCATTAATTAAATAAGCCACCTTCCTAGTTAAAACTCTAGTTTTACCACTTCCAGCTCCAGCGATGATTCTTAAAGGACCATCAAAATACATTAAAGCTTCTTTTTGTTCAGCATTTAATGAATTCAAAATTTCATCTCGTTTGCTCATATAAGTCTCCTCCTATTTTTTAATGTAAATTACAGCTTCATTATTACTTTTGAATGGTAACAGTTTGATTTCATCTAAATAGCTTGATTGTGAGTAATGTTTTAAGTATGTTACTCTGGTGTTTGTTGAACGATCAAAAAGTCCAAGTCTGGTTCTTAAAGCCATTTCCATAATGTAATTAATTAAGATTAAAGTGAATCAAAAGCCAGATAAAAATATGATTAGTTTTGAGGCAATTTGAAAACGATAATTGCCTTCATGTTTTTTAATTATGAATTGACTAAAGTCATTAAATTCATTTGGCATAACTAGTGTTATTGGGATTAAAATTATTAAAAATCAGAAAATTAAAGCAAATGCATTTCTTCTTAAAGGTAATAATTTTAAAGCTAAAGGAGTCATCTTTTTAGCAGGTATTATTTGTACTCTAAAAATCAGCATTCCAATTGTTCTACTACTATAAAACAATGGAATTAGTAAAAATAGCACAAGTAAAATAACTGTTGAAAATATAATAAAAGAATAAAAGTTTACCGCCTTGACAACACCTTTATGGACCATTAAAAAGTAATAATTTAAAATTGCTAAAGTAACAAAAAGTCCAAAGTCAAATAAATTAATTAATAGTCGATATCAAACATTAATATTTTTATGAAATTTCATTTACTATCCTATAAATTATCCAAAATATCTTCTCATTTTTTAACTTTAGGTAGTGTTTTTAAAAAGTTAGCTTCAAAATTATTTTTAATAACATAAATATTAGTATTAAAGAATAGACTGTTGTCTAAGTGTTCTTTTTCTAAGTATTTGTAAAGCTCATTAACAAAACGATCTGCACGTTTTTCATTGTAATTAATTCTGTCTTTAATAACATCTTTTGATCAGAATCTTAGTCTTCAGGTATTTAATAAAGCACCTAGAAAAATTTGTAGAAAATACATGCGAGCATAAGCTATTTCTTGCTCAAATTTTAAGTTATTCGTTTTAACATAGTTTTCAATTGCTTTAAATTGTTCAATAAATGTTTTAGGATTCAATCACAATGATGAAGGAATAAATTCACGAACAATTCTTTTGTTTGTATAAAGATAACTTTGTGCTTTAAGTAGCATAAAATAGTTTAATTCGATACAAAACTTACTATCATTAACAACAATAGAACGGTGTTTTAAAAATTGGTTAATTAAAGATTTTTTGAAAACTTTATTAAAGATAAAAGGATATGTATATGCTAAAACTTGATGATTTTCTTGAATGTTATAAAGTTTTTCTTCTTCTAATCTTGGGTTTGGTTTTCATTTAACTGTGCCAACTAATCTTGGACGATATTCTAAAACATCTGGGTTAAATTTTTCAATATAGTTTGTTAATTCTTGTGCAAAGTAAGAACGCATTGAGTTGTCACTATTAATAATTGTGGCATAGCTTCCTTTAACTAAGTGAAAAGCACAAACTATGTCACTTTGTATAGTTCTCTGGCGATTATTAGTAATAAATTTTAAACGTGTGCCGAAGAATGCTAAGTATTTTTCAATAATAGAATACATTTTAGCTGAAGGGTTTCTTAAAGCTAAAATTAATTCAAAATCTTGATTATTTTGATCTTTCATACCTTCTAAAATATTTTTTAAACTTTTAGCGCTATTTATTCCTGGTGAAATAATTGATAATTTCATTTTACTCCTTTACATTTTAATATTTCCAGATGTTCTTGGATATGGAATAACATCTCTAATATTGTCAACACCTGTGACATACATTACTAATCTTTCAAAACCAACTCCAAAACCGCTTGAACCACTATCGCCAAAACGACGCAAGTCTAAATATCATTGCAATTCTTCTTGACTGATTCCTACTTCTAAAGCTCTTTGAAGTAATTTGTCATATCTAACTTCTCTTTGGCTTCCACCTATTAATTCGCCAATTCCTGGCACTAATAAGTCAAAAGCTGCTACAGTTTCATTGTCATCATTTTGTTGCATATAGAATGCTTTAAAACTTTTTGGAAAATTAATTACAGCTACTGGTCCATTAAAGATTTTTTCTGAAATATATCTTTCATGTTCAGTTGCTAAATCAAGTCCGAATTCAATATCTTGATTTTCAAATTGGTCTTTAACTTTAGCCAATTCTTTAATTGCGTCGCGATAGTCAATAACACTAAGTTTTGTTTTTAAAAAGTGATTTAATCTTTCTAAAAGACCTTTGTCAACTGTTTCATTTAAAAATTTAAATTCAGCAGGATGTTTTTTAATTGTATTTTTGATAACAGATTTTAACATGTCATCAGCTAATTTGATTGCATCCTTTAATTCATAGAAAGCAACTTCAGGCTCAATCATTCAAAATTCTGCTGCATGTTTTTTGGTATTTGAGTGTTCTGCTCTAAAAGTAGGTGCAAAAGTATAAACTTTTTTAAATCCAATTGCATAACTTTCAGCATGCAATTGTCCCGTAACTCCTAAAGTAGCTTTTCTAGTCCCAAAGAAAGGATCTTTTGAATTTTCATCATTAACTACAAAAGTTTCTCCAGCCCCTTCACCATCATTGCTGGTAATAATAGGAGCATTGAAGTATAAGTAACCGTGTTCATCAAAATATTTATGAACTTCTTTAGCTAACGTTGATCTTATTAACATTGTAGCTCTTAAAAGATTTGTTCTATGTCTAACATGAGGAATATCTCTTAAGGTTTCTAAATTGATTCCTTGTTTTTGAATTGGAAAGTCTAAATCAGCATCTCTTAATAATTTAACTTTAGTAGCGACTAACTCTAAAGGTTGTTGAGCTTTAGGAGTTAAAGCTACTATTCCTTTAACTTCAACTGCAGCACCTAAATGAATTTGATCTAATTTGTCAAAGTCAAATTTTTCGCCTTTGCAAGTTACTTGTAATGATTGAACAGTTGAACCATCATTAATACTAATAAATCTAATTTTTTTGTTACCTCTATTTGATGTAACTCATGCTTTAATTTCTAAAGTTTTGTTTTTAAATTCTTTACTCTTCTCAAATAAACTTTTTATGTCACTTGTTGCCATATCCACCTCTAATTAATACTAAATTTATTTATTATTTATTAATAATAATTTTAGCATAGAATATTAGTATTAATATTAAATGAATTTAAAAAAACTTTAAAAAGTTGCTTCTAACAACAAAAAATTTTTGCAAAATAATTTAATTTAAAAAATTATCTATATAATAGCATAGGTTTATATTTAAAAAATAATAATTATTTTTTGGCCAATAATACTGTTTTATAGGCTAATAAGTAATTTTAAGTACATTATTAATTTATATTTTATTTAATTTATGAAGGGACAAAAATATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGAATTTTAGCTTTAAGTGGTTTAGTATTTTTCATTGTAGTATATGCAAACAAAAATGCTAGAACTAACTTAGGACCTGACAACGGTTGAATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGATATGTTTACGTAATTTTCCAATTAGTAGCAATAGCTGTTTATGTTGTACAAACTTACTTATATTATCATTTCAAAAATAAAGCTAAAAACGTTCAAAGTGAACAAGAAGTTCAAATTGAAACTAATAACTAAAATTCAAAATGATTATGAGGCTTTTAAGCCTCATTTTTATTTTTAAAAAATTAATTTAGTATAAAATAAAAAAGATTAACGTAATAAAAAAAATGGAGGCAATTATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGAATTTTTGCAGGTTTAAACATGCTTAAATTAAAAGCTACTAAAAGTTTAAATGTTGAAAATTTTGAATTTAGCCAAAATTACTTTTTATTTTGAGACAAGTTAGAAAAATCAAATACATTTTTACAATTAATGATTGATAATCCTAAATTAAAATATGAAGATCGTCTTTTTGCTTTCTTAATGGAAGGCAGTGTTAGCGATGGCGGATATTGAGAATGAGTATCAGGCTTAATTAAAAAATATGGAGCTGTTCCTAAAATTGTTATGCCTGAAACATTTAATTCAAGCAATACTCATGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGTGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAAGCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAAGATAAAAAATTTGTCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAGCATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTACTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAATCACTTAATGTTACAATGGATGTAATTAAAAAGGCTGTTATTGCTTCATTAAAAGATAATGAACCTGTTTGATTCGATTGTGATGTTGATGCTTACAGTGATCGTAAAGGTGGAATATTTGATATCGATCTTTATAAATATGAAGAAATGTTAAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCCAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGAGAAATTGAAAACTCATGAGGAGAAGATGTTGGTAAAAAAGGTTACTTCTCAATGAGTGATAGATGATTTGATGAATATGTTTTTGGAGTTATTGTAGATCCTAAATATGTAGATAAAGTTGCTCTTAAAGGTTTAGAAAAACCTGCTATTGAATTAGAGCCTTGAGATCCTTTATCATAATAAATTATTAGTATAAAAAAATAACCAATAAGGCTGTTTTATAGTTTAATTGGTTATTTTTTTAAGTTTGTCTTTTTTAAGTTTAATTGCTAGATAAATAGTAATTGAAATAAGTATTATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGACAATTAGTGGCATGTATAATCATTCTAGCCTCCTAAATTAAAACCATTAAAAAGGCAAAAGCAAATCCAAAAATTAATAATGAAGAGATAATATCAGTAAATGTATTAATTAATGAAGCTGATATTGAACTTGGATCTTTTTTAATTTTAGCTCAAATTATTGGAAATGCAGCACCTAGTAGTTTTGAAATTGTTAAAGCTAATCATAAAGCAATTGAAGAACCTATTATTATTGCTCAATAAATCATTTTGTTAGTTGACATTAAATCTCTTGTAGCTGCAAAATAAATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGAATTCCGCTATTACTTGTAGCATTATTAATTACTGGTATAAGGGCTGCAAAAGAAATTGTTGCTAATGAAATTTCGGCTACATCTTTAATTTTTTTAGCAATAGTTCATGTTCCAAAATACACTATAATTTGGGTCACTGTGGCAAAAAGAAGTAGTAATATTAATCATAAAATTCTTGATTTGATTATTTTATATCATGGAGTTTTTAAGTATTCGCTTTCATTAACTTCATGGCTACTAATACCTGCCATTTTGTATAAGTCTTCTGTAGCTTCTTCTTTAATAACATCAATAACGTCGTCACTTGTAATCATTCCGACTAATCTTTTTTCATGGTTAACTACAGGCAATACTGACATATCGTGTTCACTAAAAACCTGAGCTGCTACTTCAGTTTTATCATGAGCTGAAACATAAGTTACAGGATTATAAATATCATCAATTTTAGCATTAGGATCAGCAAAAACTATTTCTTCTAGTGTTAAAACACCAATTAGTTTATTTGTTTCATTAACTACGTAATAGTAATGCACTAATTCAGCGTTTTTCTTATGATAATCACGTCTAATTTTATGTAAAGCTTGTTCGCAAGTATATGAATTACTAATTGAAGAAATGTCTATTGACATTATGCTTCCAACTTCATCATCTTCATAGCTTAAAAGTTTATTTAATTCAGCTCTTTTTTCTTGAGGTGTATAAGCTAAGATTTTGCTTGTGACATTCGAAGGTAATTCTTCTAAAACATCAGCTAGTTCATCACTTTGTAATTCTTGTAGTAGTTTCATACCTCAATCTTCTGTAAAACTTTGAGCTAAATTAGCTTGGACTTCTTCATCAAGGTAGCTAAATAATTCAGCAGCTTCACTAGTTTTAAGTAAAGGCAAAAAAGTTAGCTGTTCTTCTAACTGTAATTCATTTAATAAGTTTGCAACATCAGCCATTGGATTATTATCAATAAATTCTCTGGCTTTAGTTACATTTTTAGTTTTAATAATTTCATGTAGTTCGTTTTGCAAACTAATCTCTTGACTTTCGCTCATTTTAATCCTTTAAGTATTTAACTAGTTGTTCTTGGAATTGTTCAAGACGAATGTTGTAGAAAGTACCATTTTTAACAAATTTAACATCCCCTGTTTCTTCACTAACAATTATTGTGGTAGCATCACAAAGTTCACTAATTCCCATAGCAGCTCTATGACGAGCACCATATTGATTATCAATTGATTTTCTGGTGATTTTATAAAAAGTAGAAGCATAATAAATTTTATTATCTCTTATAATTACAGCTCCATCATGCAATGGACTATATTTATTGAATATTGAAATAAGTAAGCTACTACTGATATTAGCATTTAAAATTACCCCATCAGTTCTCAAATTGTCAATATTATCATTGTTTTCAATTGTAATTAAAGCTCCAATTTTATTTTTTGATAAATATTCAACAGCTTCACGTAGTTGGTTAATTAATCTAATTTGACTACTTTGACCTAATTTTTGGAAATGACTTCTAGCCATTTTGTTTTTAATTGCATTAACTATAAGTGGCATTATTGAAATTAAAATTAAAGTAACTAATAATGCAACAATTACATAAAAACCAATTTTAGATTCTAAACACATATTTTGCTCCTAAGCATAAACTCTTGAAATTAAAACAGCTAGTAGTGTGCAAAGTAAAATAATTAAAACTGATAAGCTGGCTCCTAAAACTTTAAGAATAATTTTTTGCTTTCTAACTTTAGGATCTTCTTTCTCGTGTTTATTTACTAAACACATAAATTCCTGACATTCACCAGTAATAGGTTTTTCATCTTCAATCAAAGCTGGATTAGCTAATTCAGCTTGATTTTCATTATAAAGTTTTAATAGTAATTCTCTTCTTTTTTGAATTCTTTCAGCACATGTTGCTAGATCTCTATCAATAATTGCCATATACACCTCTTTCAATCAATATTAAATTTAATACTAATAATTAATATTTTATATTATATTCCATTAAATTGAAATATATTAGTTACGAAGTAGCTAATTTTATTCAACAACTTCAATAATTAATTTAACTTTTGGTCATATTTTTAGGTATTATCTTATTTTGTTCTGCAATATTATTAGGTGTTGATGTAAGGTTAATTCCTTTCTATTGAGAAAGAGAGAAAATTTACACAAAATTCTAAACAGTATCCTCCCTTAAATTTTTCATAATCTAATTATAAACTAATTGAATAAGTTGCTTTTACTTTTTATATAATTTGCACAAAATATCTATAAAAAAGGGTAATTGGTAAATTATAAGTTGTAACTTATTATGGTCCAATTACCCTTTTTTCTTGAACCATTTCTTTCGATCAATTTTTTATTTTTAAGCTGGTTAATAATTTTTTTAATATATCCTTCGCTCAAAGATAAGGTACTGCTTAATTGATTTATTGTTATATTCGATTCTTTTTCTATAGTTTTAATTACTTTGATTTGATTATCATTTAAATTATTTTTATTTTCTTTTTCATTTATTCTTTTAAATCCTTTACGATTAAAAGGAATTTTTACTCAAATTGTATTTTCGTCAATTTCAAATGCTTCTTCACCGTATTTTTTAACAATTAAAGGCACTCCATGTCCAGTTTGTTCAACTAGATCCAATTGCATAAAAATTTTTTGTAATTTTTCATTTACTGGTCTACTTACGCCTTTGAAAAATTCCTCTTTAGTCATATTTCTAGGAATTCCGCCATTTGATTCTATTTGAATATAGTCTTTGAATATATTAACTTGAGGTGGATATTTTTCATCTCAACGATTATGAATGCATGCATTTAGTCATGCTTCTCTAAAACACTCAAAATCAAATAATTTTTGTTCACTCCTGGTTGCAGTTGACAAATCTACATAAGTATCATTAATAGAACCTACATAATTTAAAACACTCTCAATTGCATAAAATAAACATTTATTTCCATATTCATTTCTTTTCAACAAAACACTTTTATCATTATCTTTAAAAGTATTAACTTTAATTGAAATATCATTATTATCTGCTAATAAATAAGACATTAAATTGTATTTATTATTTTTAGTCTTTAAATCAAATTTTTTTTCGAAAGTATCTTTATTTACTGTTAAATTTTTAATATTAGCATATAGCAAAAAACTATTAAATGTCAAATCTTGATCTTTGTTTTCAATTTCACAAATATTTATTTTAGAAACTGATTTTTGCACAAAATCTACTAATTTATCTCTATTAAGTTTTTTATCTTCATCTCCAATACGAATATAATATTTATCATAAGCTGAATAAGGTTTTTGTTCTCCTTCAGCTTCAACTTTAATTACATTTTGGTTATCAATAAATTCAATCGAAATTCTAGGCATTATTTCTGGCTTAACATTGTTTGAAATAGCTTGAGAAATATCTCTCAAGGTTTTATTGCCAATTTCTAAACCAACAACTGTACGATCATCTTTTACGCCAAAATAAACTGTTCCCATTCCTCTTTTATTGAGCATTGCAGTTAAAGAAATAATTGCTTCTTTTAATTCACTAGTTGATTTTTTGTATTCAACTTCTTCACTTTCTCATGGCATTTTATTCATATTTATCACCTTATAATTTAATTATAAACTATTTTTTTATTTATCACTTTAATTGCTACTTTATTTATTACTCTTTTAAAAATTTAAAAACTAGACTTAAAATTGATTCTTTATTATAAAAATAATTAAATATTTATATTTAAAAAAGTCTATAATAAAAAGATATGCTTATTTATCTAAAGATAGAAAGGAATTAAGATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTTTAAGTGCCTTTTTCGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAATATGAATGAAGTAAGTTTACTATTTTGAAATATAACAACTAGTGGTACTAGTCAAAGCATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGGGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGAGGACTTGTTTTTACAACCACAACTAATGTTTATTTATCAATAGCTATTTACATTATTATTCCTTTTTTAGTAGGAGCAGCAGTTTTTGCAATTTTTAAATTATTTCCTTACTATCGTAAAGAAAACTGAATTTTAGATCAACTTAATGAATTATCAAAAGAAGATATTAATGCTATCGCTTTAATTAAATCATATAACCTTGAAGAACCACGTTATGAAAAATACTTTAATAAAAATCAGGAATGATTTACTATTTATCGTAAAAATGTTGTTTTTGGTTCATTTGCTTGACCAATAATTAATGGATTTGTGCTTTTTGGCAATGTAGCCATTTTTGCTCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTCACAGCAAATTTAAGCATGATTTCAATGGGTGTTTTCAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAATAACTAGTGGAGCTATTGAATTTAAGAATGTTAATCTTGCCTATCCTATCTATAAAAAAGGCGAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGTCAAAAAATTGGAATTATAGGCAAAACCGGAAGTGGTAAAAGTACACTTGTTAAACTTTTAACCCATGAATATTTACTAGATAAAAAAGATGGTGAAATCATTATTGACTCGCACAATATTAATGAAATAGATACTAATAACTTTTATGAAAAAGTTACTCAAGTTTATCAAAAACCAATGATTATTTCAGGCTCAATTAAAGAAAATATTACTTTCTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAAAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCATTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAACAAAACTTAAAAGATAGTACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAGATCAAATCTTTGTTCTTGATAAGGGACAAATTGCTGCTAGTGGTAAACACAACCAACTATTAAACACAAGTTCTATATATAAAGATATATGACAATCTCAAGAACAAAAAGGAGGTGAATAATGCCTCGATTTAGTAATTTAAGATTTAGACAAAAAGGTGAAAAGAAAATCAGCTTTAAAATTTTTTTCAAATTTTTAAGTAACTTCCAAATTTCAAAAAGTGTTTGAATTTTTACAGTGCTTTTTGGACTTTTAGTGGCTAGCTTAACTAATATTGCTTCTTGATTAATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATGAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAACTTAGTGCTCGCCTTGGTCGGAATTTAAGATTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGCTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCTTTAACTAATGATATTCAAAATATAACTGAAGCAATCTGAGACATTTTAATTTCAGTTATATCAATAATATTTGAATTTAGCTTTATTACAATTATGATGTTTATTTATGCTCCACTTTTAGCAGCTATTATTTTAGTAATTGTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGATTCCATTTTAGTAAAACTATTAATTTAATTATTGTAATTGCTGCTAGTGCTGCCTTTGTTTACTACAAAGTTCCAACAGGTGGCATTAAACCTTTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGAAGAAACTATATTGATACTATTTTGCAAGAAACTTATATTTTTAATGATACAGTCAAAAATAATTTAACTATTGTTAATCCAAATATAAAGGATAAAGACTTTAATGACATAGTAGAAAAAACTTCAGTAGATGAATTTATTAATTTAATGCCTGAAAAATTTGACACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGCCAAAAGCAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAACATTGATACAATAACTGAATTAAAAGTGCAACATGCAATGAAATATTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAATACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGATCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTGAATGATACAAAAATTGATTAGTAAAAATAGTCAGCTGCGATACTTTTGCGGCTGATTTTAATTTTTGTTTAGAATATCTAAATCAAGTAAAAATTGTTTGATTCCATAAATTTTATAACCATTAGTATCGGTATCAAAAATAGGGTCATCTTTAATAATAATAATTTTTTTAAAATTATCATTGATGTTTTTAAAAGGTCTTTTTTCTTGAATTTCTTTATTTTGATCATACATATTTAAATAAGATTGAATATAAACTTTTTGATTACCTTTTTGGGCAATAAAATCTACTTCTAATTGAACTTTCTGATTAGCATTAGGTCTTATTTCAACTATTCCAACATTAACATTGTAACCTCTTAAAAGCAATTCATTATAAATGATATTTTCCATTAAATGATTTTCTTCATTTTGTGCAAAATTGATTTTTGCATTTCTTAAGGCTATATCTTCAAAATAATATTTGTATGAAGCATCAATATACTTTTTACCTTTTATGTCATATTTCTTGACTTCATTAATTATGAAAGCATTTTTTAAATTTTCAATATATTTTCTAATTGTTGCATCTGAAATATTTGTTTTCTTAATTGATTTAAAAGTTAATGATAATTTATAAGAATTAGTGTATGAACCTATATTAGAAGCTAAAATATTGATTATGTCATCTAAGTTATCAATATCTTCTATTTTATTTTTTTCAACTATATCTATTAAATAAGTTCTTTTGAATTCATTTACCAAATAATTAGTTTTAGTGTCGGCTTCTTTCATGTTTTGAATTAATGGCAAACCTCCATAAATTAAATATGATTTCCATAACTCATCAAGGTTTTTAAATTCTTTGTGTGAGGTTATTTTATAGAAAGATAAGGGAAAAACATGAAACGAAATCGATCTACCTCTAAATTCTGTAACTACATCATTAATTAAGAATTTAGAATTGGAGTCAGTGACAAAAACATTAAGTTGTTGATTATTATTAAGTTAATTTAAAATAGGCACAAAATCTTTTAAAAGTTGCACTTCATCAATTAAAAGAAAATATTTTTTCGATCTTTAATTTTACTTTTTATAAATTCCAAAAAATTATCAAGATCACAATAAGAATGATATTCTTGATTTTCTAAATTAATTTCAATAATATGATTTTGTTCAACTTTTAATTCATTTATTAAATATTCCTTGAATAAATTAAATAACCAAAACGGCTTTCAACTTCTTCTTAAGCTTGAAATTATTTTAATAAGAGGTATATCAAACATCTTTTTAAGTCTTTCTAAATATATATCCAGTTTAATTATCATAATTACCTTTCATAATGAAACTTTGACACTTTTGTAAAAGTTTCATTATGAAAAAAATAAAAATCAAAACTAGGCTATTAACCCAGTCTCAATATATGGTGCCCGAGACAGGACTTGAACCTGCAAGCCGTTAAGCACTAGATCCTTAGTCTAGCGTGTTTGCCAATTTCACCACTCGGGCATAATAAGTAAATTTATTTTACCAAAAGATTAACTTTTTAGTTAAATAAATTTTTACTTTTTTTATATTTCTTTTAAATCTTTAAATAGCTTGGCTATTCCACTATTTTCAAAAGAAGGCCCTATATAATGTGCTACTTCTTTTACCTCATCCATTGCATTTTCCATAGCAATGAATGTTCCTATCTCAGGTAAAGCATTTAAATCATTTCCGCTATCACCAATGTGAGCAGCGTTTTTAGGATCTATTTTTAATAAGTCACAAACAAATGCTGCACCTTTACCTTTGGTTGCATTTTTATGTGTGATTTCAATTGAGTAACCTTTTGAAACTATATGAAATGCAAGTGAAGGATATTTTTTAGCTAAAAAGTTCTTAAAATTTTTAGTAGCTATTTCATCCTTACCAAAAGTTAAAATTTTAGTTACATTAGTTTTTTGACCTAAATCACTATATTTCAATTTTTCTAATTTAGCAGTTCAAGGGCGCATGTAGGCACTTGATTCATAGTTACAATAGATTTTATTTTCGCCATTAAAAATAAAAAACATTTCTTCTTCTTTTAAAAGCTTAACTATTTCATCAACTTGATTTTGTTCCATCTCATATCTAGCCAAAACTTTATTTTTCTTATCAACTATTAAAGCTCCATTTTGACAAATGGCATAAGGGCACTTTAACAATTTAACTAAGGATAAAACAAATTCGCTATTACTTCTACCTGTTGAAATAATAACTGGTTTAGTTTTATTTAATTTAACAACAGCTTTAACATTAGCACTTGAAAGAGTTTCTTCACCTTCTGGTAAGTCTAATAATGTTCCATCTAAATCTATAAAATAAGCTTGAATTTCTTTTCTCATGATTTAATTATATATAAAGATTTTATATATAGAACAAAAAAATATATCTATAATTCCTAGATTTTTAGTCATTGACTAATAATGCTGTTTTATAGATATATTGGTAAAATATGTTCATTTAAAAATTAATTTTCTTGCTTAATTAAACTATTTTAATTTTCTTTATTTTTTAGTTTGATCTTCATCATAATCACTATTTTTATTTTCAACTTCTGTTATAGCAGCAGCATAGCAATAGAATCAATCTGCATTTCTATTAAATATTTTTGAAAAAGCATTCATTTTTTCTAAATATATATTTTGAACGTCTTTTCAAAGACCGTACTCTTTACATTCTTCTACTCTTTTCATTAAAGAATTTGTATTAGGAATGTTATATCAAAATCATCTTTCTTTTTGAGCATCTGATTTTTTAAAATGTGCAAGTTGAATTCAATATTCTGGTCCAAATTCAAAAACAATATTTAAATTAATTATAAAAGGATTGTTTTTGTAATCTCCAATTGTTAGCTTTTCAGTTGTTAATGAAAGAGGACAATCTTTTGATATTTCGCAGCCAAGCTGATTTAATGATTCATCAAAATTCTTTTTTACAAATTCTCTTATCTTTTTACAATCATATAAACTGTTGCCTTCAAAAAAAGCGTAAACAATAAGGCTATAATCTATTATAATTACGCCTTCATCATTTTGAACTATTAATTTTTCACTTCCACTGCCAAATAGCTCTTTTTGAACTCTAAATATTTTTGTTTATTGATTCTTTTGATCAAATCCTTAATCAAAGTTGAACCAAATGATTCAATGTTTTTAATTAAAGATTTATTTTTTACAAAACTGTACAAATTTACCTCCTGTACTTATTAATAATATCTAATTAAATTTTATTAAATTTCTTTTTCTTTTTTTATAACTTTATTAACTTGACAAATTGTTAAGAATTTTAAATAAGACAATAATGCAATAGTTAAACCTAAAACATAATAAGATTCAACCTTAAATGAATTAACTAAATCGCTAGTTTTAGAATCACTTATTTCTTTAGTTTTATTTAGATAAGGTAATACTTTTGTTATAGAAGTATAAATATCACTAAATAACTCAAATTTGGGATTATTGAAAATAAAACATTGAACAAATTGTTCATTTCAATACTCGTGACAATAGTCTTTAATGTTGATACATTTATTTAAAGCATCTTCTTTACTATATGCTAATGATTAAAGTTTGATAAAGTACAATAAAGTGCTTTATAAAGATAAATATAGAATTCTAAAATCTTTATAGTTTCACTGTCATTACTTGAATAAAAAGGTTGAAGATCTTTAACAAAAATTTTATTAATTTTTAAATCTTGAAATTTAGCTAAAAATTCATTATATTTTTCTTTTGCCAACTTTTGGAAACTATTATTTGAAACAGTTTCAAAATTGTAATTTTCAGTATCTTGATAATCAACATATTTATTTTTGAATACTAAAATATCCGCTTTATTTAATTTGTCATTTAATGCTTTAGCAATTGAGTCTTGTAAAGACTGAATAAATTTATAAAATGAATGTTTAATTAAATAATCAATTGCTTCACTATCTTCATTGTTGTCTTTACTATTCTTATTCTTTTCATATTTGTTAGCATTATTAAATCAGATTTTAAATGCTTCTAAATTAGTAGCATTATTTAAACCTTTAGCGAAAAATGGATAATAGTCTTCATGTCAAATTCAAAATGAAAATTGATTATCTTCTAATCACATTAGGTTTCTAAATCATTCTTCTTTACCTAATTCTCTTTCAGCTTCTTTTTCACTTTCATATTTAAGTCTAAAAGCATAATATTTATTATCATAATGAGGCAAGAAATAAATTCTTATTTTTTCATTAGTATTAGTGACTTTTTCCTTGATATATTTTTTTAATAATTCATAATAATATTCATCAAAAGCAGTCCAATCATCTAAATGAATTCTATTTTCTTGATTTTGACTATAATCATATAAATATTCATTAAATTGCATTTGTGTAATTGTTATATCTAATTTCATAATCTCCTTTATTAGCTATTTAATTATATAAAAGGAGTATTCAATAATGTATATTATTGGAAATTGTGCAATAAAACATATCCTAGGTATTCTAGAATATGCTAAAAAATTATTTTGTATTTTTAAATGCATCTTTTTTTAAGACTATTGATTGTTAAGTTTGAGAAGTATTCTTGATCTATTTCTTCATTATAGGTTATTATCTAGCTATATTAAAACTAACTTAAAAATATATTATAATATTTACTATTATAAGGGAGGCTAAATGAAAGCAAACGATGAAAATATGTTAGATTATATGTTTATGAATCGTAGACAATATCACATACCTGTTTATCAAAGAAATTATGACTGAAAAAAAGAAAATTGTATACTGCTTTATAACGACATTATTCAAGCGGCTAAAAATGACAAAAAACATTTCTTTGGTTCTATAGTTCAAGTCAATTTGAAAGAAGAAAATGGCGTAAACAATTATTTAATAATTGATGGGCAACAAAGAATGACATCAGTTTATCTTTTATTAAAAGCGTTATACGATTTAGAAACTCATCCATTTAATAAAGAGGATTTAGAAAGATTACTATTCAATTTTAGAGATTCAAAGAAATATTCAAATGATTTAATAGGAAAATTAAAATTAAAACCAATAAAAACAGATAACAAACAATTTGAATATTTAATGTCTGGTAATGTTGAAAAAATGGATGATACTTCAAACATTAAAATTAATTATGAATTATTTAAATTGCTAATAAAAGATTCAATTAAATGCAAAATAGAAACTTCTAAAATTTTAAGGGGAATAAAAAATTTAGAAGTTGTTATGATTACTTTGCAAGAAGAAAAAAATGGATTAGGTGACAATCCACAAGAAGTATTTGAAAGAATTAATTCAACTGGAGAAGATTTGACTTTATCTGATTTAATAAGAAACTTTGCATTAATGACTGATCTAGATAGAGAACAACTATTCGAAAATTATTGATTGCCTGTAGAAAATACTATTAAAAAAGATAAACTTAATGATTTTGTTTTAGCATATTTACTTTTCAAAATGCCAGACTCAACAACTGAAAAAAATGCATATCAAACTTTTAAAAAATATGTCGAAAAAAATCAAATTAGCAACAAAGAAATATTGGAAGATTTGAAAAAATATTCTAAATATTACAATGTTTTTATTAATGATGACTATAAAAATTATTCTAAAAAAACAAATAATTTATTAAGTGTTTTCAGAGTATTAAAACAAACAACAATTTATCCATTTTTATTTAGTGTTTTTGAAGATTATGAAAATTCAATAATAGATGAAAATGTTTTAAACAGTGTTCTACAATTTTTTGCCACATACATTATTAGAAGATCAATATGTTCTATTCCAACTAATTCCTTAAGAGGGTTGTTCAAAACACTTTACAAAAGAATTTTTCAAAATGAAAAAAGCAAAGAAGAATATTTAAAAAATTTATATGGCTTTATGGCTTCATTAACAAATACTAAAGATAGTGTTCCATCTAATGAAAATTTTGAAGATTCATTGAAAAAATTTGAATTGTACAAAAATTCAAAATTATGTAAATACTTACTTTCAACAATAGAAAATGGAAATTTGAATTTCAAAGAAAGAATTGAAATCAATGAAAACATTACTATTGAACACATTATGCCTCGTAACAAAACCTCTTATTGAAGAAATGAGATTGGTGCAAATTATGATGCAGTTTACGATAAATATTTAAACACAATTGGAAATTTAACTCTAACAGGATACAATTCAGAATTGTCTGATAAACCTTTTAAAAGTAAGAAAATTTCAATTGAAGAATTTGGTAAATTTAACACATTGAACAAAGATATTAAAAATTGTACAATATGAAATGAAGATTCAATTGTAAAAAGAGCAAATAGATTGTCTAATATTGTTTGTGAAATATTTTCTTTACCTCAAGAAGTATTTGACTATAAAATAGTAGAAGATGAAATAAAACAAATTAATATTGATGACAAAATTGATTTAACTTTCACAAAACCTGTTTATTTCAACTTATTAGGTGAAATGGTTGATTCAACTTCTTATGGTGAGCTAATTAAAAAAGTATCAAATATTTTATATTATCAAGATCCAATGACAATGAAAAAATTGGCTCAAGATAACTACAAACTTCCTAATGGAACAAGTGTACAAATAACAAATGATCCTAAAAAATTAAACAAAGCTATAGAAATCGATGAAAATAGTGGAATTTACATTGAAGGCACAAAAAATGCAAATGCTGTTATAACATATATTAAAGCAATTTTATCAGAATTTGATATTTCACATGATGACTTCATTGTATTTACAAAAAAAGTTAAAAATAAATAAAGAAAATAATTTAAAAAACTAAATTGTAAAGAAATATTACACCTATTATTTGGAATATCGCATGCGATATTCCTTTTAATTTATTAATTCTATCTGTATTGTTTTTGTTTTTTTGCGACAAATAAAAAAGTACAAGCTTTTAAAAAAAGACTATGCATTTAGATTTTATCTAAAAATGGAAAATGTTTAGAATTTTATGTAAAGTCAACTTCTCTATATTGAAAAAAGGATAGAATTTACACAAAATTCTAACAGTCTCATATCATTTACCAATTGTTTAAAATCTGGACACACTTGTATGTCATCTATTAATAAATAGTTGACTTTATCAACTTTATGTTTTGCAAAAATGTAATCTTTTGCTTCTTGATAAGTTTTAAATTTTTTGATGTAAAAATCATCAATATAAATTAGATTTGAGTTATCTATATTTTTTTCAATATAGTCGGCTAATTGTTTAAGTATTGTACTTTTTCCAACTGATTTGTAACCAGTTACAAGTTTAATTTTGGTGTTATTTAGATTATTAATCAATTTGTCTAAATATAATTTTCCAGTTAAAATTTTATGCATTTTGATCTTCTACAATTTGTGATTGTTGGTTACTAAAAATACTATAAAATAGGCATATTCAAAAAATTTATATTTAAAAATATAAACGTTTTGTAATTAGAAAATTTCTTATTATCAAAGAACATTATTGTTCATTATATAAATCTATAAGGTTTTGAATCGCTTCACTAACTTGAACTTTGATGGTTTCTCTTCTTTTATCAAAAAGTCCAAGAGCAGGTAAAATATTTGTGAATTTTGTTCCATAGTTATTAAGTTCATTGTTTTCAATAGCGTTAAGAATAAACTTTTTAGCTTCTTTAACTTTAAGATTAAATTTAGCTATTAATTCATTAACTGCCACTTCTTTATCTAAAGCAACAAATTTGTCAAAGGCTTCTTTTAAGTCGAGAATACTTAATTTGTCAAAATCATTATCGAAGTTGACTGTACGAATAAATCTTTTAATTAATTCTTCTTTGTTTTTCAAGTTTAAACTTGATTTAACAATTCTCTCAATTTGAACTATCAATTCTTTTTCAGTAATATTTTTTTCATGTGCATCCTTAACTTGGCCTAAGATGTAATCTACATTAACATCATTAGCTTTAACAAGTTCAATTTGAAAGACAACATCATCCAAAATTGAAACTGCTTCACTTTTTTGACGAGTTTCAAATTCATCTTTTATTTCTAAATAAAGACTTTGATAGTCTTGCATTTCACGTTCAGTTAGAATTTGTTTATCAGGAGTTCACTCTTCAGTAAAGACTTTAAGAATGTTCAAAAGATAAAGTATTTGACCAAAGGTTTTAACAAATAATTTTTTAGCTGGATCTTCATAGATATATTGATCAAGAGGAAATTCATGTTGAAGTCTATTGACTAAACTTAAATATGAAGGCATTTTAGGACTAGCACCATTACGGTAGTAATCATCAAATGATTTAACAATAATTAATCCTGTTGAATCTTTATCTCCATAAGCTGCAAAAGCTTGATCAACAAATCATTTTAAATTACGGTAGCAAATGATATTACCAAATTGTTTTACAGAATTTAATATTCTGTTTGTTCTTGAAAATGCTTGAATTAATCCATGATTTCTTAATCATTTGTCTACTCATAAAGTATTTAATGTTGTGGCATCAAAACCTGTTAAAAACATATTGACAACTATTAAAAGATCAATTTCTTTGTTTTTCATTCTTAAACTTACATCTTTATAGTAGTTATTAAATTTGTCATCACTAATATCATAATTAGTGCCAAACATTTTGTTGTAATCTTTAATAGCATTAGATAAAAATTCTTTAGAGCTAGGATCTAAATTTTTAGTTGATTCATTGTTTTCATCAGTTTCACTCAAATAATCTAAATCAGGTTCATCATATTTATTTTCATTTGGGTTTCAACTATAAATTAAAGCAATTCTAAGACGTTCACTTTCTGGAAGTTTAGATTGTTGTACTTTAAATTCTTTATAGTAAGCTTTAGCTGAATCAATTGAAGAAGTTGCAAGCATTCCATTGAAACCTTCAAATTCTTTCAATAATTTAACTTCTTTTTCTCCTCTATTGATTGTTTCTTCAATATTGGAAATAGCATAAAAAGAATATTTATTATCATTTCTTCTAGTTTTAGCTTTATAGTTTTTAAGAATATCTTGGACTATAGCTTCAATTCTTGCTGGAGCTCTAAGAGCTTCTTCAGCTTTAATATCTGGAACAAGTTGAGATTCAATTTGTTCTTTTGCCTTAATAGTATTAATGTAATCAATTTTAAAAGATAAAACATTATTATCTTTGATGGCGTCGACAATAGTATAAGCATGAAGTCTTGTGCCAAAGATTTCATCGGTTGTTAAAGGAATTCCACCACCTCTAGGCATTTTGGCAAATCTTGAAAGAATAGGAGTGCCAGTAAAGCCAAAGATATAATAATTTTTAAATTTTTTAATAATATTTTTGTGCATATCGCCAAATTGTGAACGGTGACATTCATCAAAAATAATGACTACATTTTTATTGTAAATAGGATGAGTTGCATATTTTTTAACTAGATTTGAAAGTTTATTAATTGTTGTAATAATAACTTTTGAATTTAAACCAGTTGAAGTTAACTTGTCATTCAATATTGAAGTATTAGTAGTTGCATTAGCTGCACCTTTTTCAAATTTTTCATATTCAACCATTGTTTGATAGTCTAAATCTTTTCTATCGACAACAAATAGTACTTTATCAATAAAGTTCATGTCTCTAATTAAAGTAGCTGTTTTAAATGAGGTTAAGGTTTTACCACTACCAGTTGAGTGTCAGATGTAACCGCCTGCGTCTCTAGTACCTTGTTGTTTCATATTGTAAGCAGCTCTAATTTTTTCAATAATTCGTTCAGTAGCAACAATTTGATATGGTCTCATAACCATTAAGTATTTTTCACTAGTGAAAACACAATATTTTGTAAGTATGTTTAAAATAGTATTTTTAGCAAAGAAGGTATTAGTAAAATCAACTAAGTCAAGGATATTTTTATTGTTTGCATCAGCTCAGAATGAAGTAAATTCAAATGAATTACTTTTTCTAAGTTGACGAGTAAAGCTATTTCTTTTGTTATTTTTATTATTTTCGGAAATAACCTGAGATCTTGTTGTGTTTGAATAATATTTTGTTAAGGTTCCATTTGAAATAACAAATACTTGAACATATTGGAATAATGATTGAGGGATGTTGAAACTTTCATTTGCATATCTTTTAATTTGATTAAAAGCTTGTCGAAGCTCAACGCCTCTTCTTTTTAATTCAATATGAACTAAAGGTAAACCATTAACTAAAATAGTAACATCATAAATGTTTTTTCTAACATAATGAGTTGGCTCATATTGTTCAATAACTTGTAATGTGTTTTTATGAATATTTTCTTTATTTATTAAGTAAATATTTTTAGATTTGCCATTATCAAAAGTAAAATTGTAAATATAATCTTTTTGAATTATTTCAGTTTTTCTTTCAATTGATTCTCTTTCATTACAAATATAGTTATTGTAAATAATTTTTCATTCACGATCATTAAATTTAACATCATTTAATTTTTCAATTTGTTTTCTTAAATTTAAGATTAAATCATTTTCATCTCTAATGTTTATTTTTTCATATCCTTGTTGAAGTAGTTGCTTAACAAAACTTGCTTCCAATTGAGCTTCGCTTTCATATTCAGAAAATTTTTCTTTAGTTGAAATATACTCAGCTAGAACTGTACTATCTGAGTTTTGCATTATAAGCTCTTTTTCTTTAATTTGATTAGTTTTCATAATGACCTTTCTTTACTAATATCACTATTTTATAGTGTTATTCATTATTTTTAAAGTTTAATAATTGATCTCTATAATACTTAAATTGTTTACTTCTTAATTCTATTTCAGCAGGTAAACCTGAATTAATTTCTTGAGAATATTCTGATAGTTTGTCAAGAATTGAAACTATCTTGTTTTGGGTTTTGAGAGGAGGAATAGGTATTTTTATTTTTGACATCTTTTCTTTTGATAAATTAAATCTAGTTACACCATTTATACATTTGAGAAGTAAGTCCCTAAAGTCTTTACAATTAAAAAAATATTTAGAAAATTTAGGCAAAAATAATTCATTATCTTTAAATCTAAAAATAAACGAAAAACTATTTAAATAAACTTTTTCATCACTTTTTATAGTGTAAACAGATGCATAACCTGTTTCATCAAAATTTTCTGATGAACCACAAAAAATAACATCGCCATAATTAAGATTGTTTTGATTTTCATCATCAAATATTTTTACAAAATTGTTAGGTAAATGATTAATTTCTTTGTTATTGAAAACATCTAAATAAGAAATATATCTTTGGTTTCCATCGACAAAACTATCTTTATTTTTTGATCTTAAACCACTAATAAAACAGCCTATAGATTGTAATTCTTTTGAATTATTTTTATTAAAGTTGCTAAAATTTAACAATTTGTTTATTCAGAAATCAAATTGCTTCCCTCTCGCTTCCAACTCCGCTTCTAGTATACGAAATCTCTCTAAGATTTCGACTATTTTTTCTTGAATTTTTAAAGGAATTAAAGGAATTTTAATATTTTCTATATGTTTTCTGGTTAATTGTGGAATACCCGATTTTGACGAAGGACTTATATTCAAGTTTTTCAAAAAATAATAAATAAATTTACTATTATGTCAAGTTGGATGAATTGCCAGAAGTCTAATAATTGGGTAAAAAGGTTCATTTTGGTATTCACAATAGCCTATTGTTCCTCTTGCAGAAATTGTTATTGAATTACCAGGCACTACATAGGAATCGGTATATCCTTTAATATTATTTCCAAAAGATCCATTAGTTAAAATTTTTACATTGAAATTTTCATTTTTTGTTTCTGAAAAAGATTTTTTTGGTAAATCTCCGCCTACATAAATTTTAGTAATATCTTTTAGATCCTTCCACTCATATCCGTCAGGACAATACTTATTTATCAATTTTTCTAAATAAGTCATATTAATCCTCAATTTCTTCAATTATTTTGTCAATTTCTTTTCTTAAAACATCTATTTTCTTAGTTAATTCTTTTAATTGTGCATTAACTTGAACAATATCAATTTTCTCTGAAGTATCTTTTGCTTTAACATAAGTAGAAACTGATAAGCTATAATCATTTTCTTTTACTTCTTCATAAGGTACATTTCTTGAAACATATTCTTTTTCTTTAGTATAACGATAAATATCAAGAATGTTATTGATATTTTCATCAGTTAGTTTATTGTTATTTTTTTCTTTAATAAATTCATTTGAAGCATTAATAAATAAGATGTCGCTATCTTTTTTGTTTTTTCTTAAAACAATAATGCAGGTTGCTATTGAAGTACCAAAGAATAAGTTTGAAGGAAGTTGAATAATAGCATCTACATAATTATTATCAATTAAGTATTGTCTAATTTTTTGTTCAGCACCACCACGATAAAGAACACCAGGGAACTCAACTATAGCAGCTGTTCCTTTAGCACTCAATAAGTGTAGAATGTGCATAACAAAGGCTAAGTCAGCTTTTGATTTAGGAGCTAAAACTCCAGCTGGGGCAAATCTTCGATCATTAATTAAAGTAGTATTAGTATCACCATCTCATTTAATAGAATAAGGAGGATTAGATACTATTGCTTCGAAATTCTTAACATTTTCAAAATATTGAGGATGATTTAATGTATCTCCATGTTTAATTGAGAAATCAGCAAAGTTAATACCGTGTAAAAACATATTAATTCTTGCTAAGTTATAAGTTGTTAAATTAATTTCTTGTCCAAAAAAACCTTTTGTAACCTTATCTTTACCTAAAATCTTAGCAAATTTAAGCAATAATGAACCTGAACCACAAGCAGGGTCGTAGACACTATCAACTTCTTTTTTCATTTCTCTTTTATCACTACTAAAATCAACTAATGTTAAAGAAGCTAAAAGCTCACTAACTTCTTGAGGTGTAAAGAATTCACCACCACTTTTACCGGCATTTGCTGCATACATGGTCATTAAATATTCATATGCATCGCCAAAAGCGTCAATGTTGTGACCATGGAAATCTCCTAGTTGCATTTCATTGATTGCTTCAAGAATTCCACTTAATTTTTTGTTTCTTTCAATAACATTATTCCCTAATTTATCATTGTTAACATTAACATCATTAAATAAACCTTTAATGTCATTTTCACTTGGTGTACCAATAGCTGAAGCTTCAATACCTTTAAAAATATTGTCTAATGTTTCATTTAAATTTAAATCATTGCGACAATTCTTAGCAACATTTTTAAATAGTTTACTTGGTAAAATAAAGAAACCTTTTTCTTGAATTAATGTGTTTCTTATTTCATTTGCTTGTTTATCACTAATTTCAGCATAATCAAAATCTTTAACACCAGCTTCTTCTTGTTTTGTGTTAACAAAATTGGTTAAATTTTCAGAAATATAACGATAGAATAAAAATCCCAAAACGTACTGTTTAAAGTCTCATCCGTCAACTGAACCTCGTAAATCATTAGCTATTTTTCAAATAGTTTTATGTAGTTCAGCTCTTTGAGCTTCTTTTGAATTATTTATAATTTTTGCCATATTTAATTCCTTCCTTTTTTTCATCTTGACTTTTAGTCAAGTAGTATTCAATTCTTTTTTTCTCTAAGTCAATAGCTTCTTGATACAAGTTATTAACTTTATAGGAGTTATCAACATAATCAATTATTCTTTGTTGTTCATTTAATGAAATTACTTTGACATTAAGTGAGCCTAGTATCTTATTATTTATATGCTTTGATACTGATCCAGCTGATTTAAAGACCAATAAATCTTTAATTACATCAATGCTTAATAAATAAGCTAAGTATTTAGGTAATAATCCTTCTAGGTTTCTAATCACTATAAAATTAGTACTAATTAAAATACCTTGATCTTCTTTTTTTATTAAGGCAACTTCATAAGGATACATGTTTCGAACAATGATGTCGCCTTCTTTAGTAAAAAATTGACTATCAATATGATCATTAGAATAAAAATTATCTAATTGGCTATTGATCACATTTAGATTTTCATCAATTGATTTGATTGTCAATAATTTGTAACAAATTGCTTCTTTGTTTTCGACAGTTTTTTTACGTGAAAGAAGTCAACCCGTACTAATTTGCGATATGTCTTTTAATTCCATTAAATACCTCCGCAAAATTATTATATTACTATTTATTTAAAATTGTTATAAATATTTTAGAACAAAAGAATTTAATATTAAAAATAACTGATTTAAACAATTTTGGCTTCTTTCGTGTTTAAAAAAATTACAAATATGTTTATAATCCCTTTGCTATTTTGCTTAGGCTGCAAAGAAAAATCTTTTTGTTAATCTTTGATTGATTTTAGAAATAATACTAATGCTATATCAAAATTTTGTTTAGTATTACAAACAAAAAAATATTAAAATATTGTTTTAATTATAAAAACTCTTTATTTTTAATTCAGCTTAAGTGAAGTTAAAAATGTTTAAATTCATGCAAATAATTATTTAATCAAGTACACCCATTTGTCTATATCATTCCATTGTTTTTTTATTTTCTTTTTCTCTTTCAAAAAATTCTCTTTTTTCATCAATCATTTTAGAAACTATTTCACATTTATTTTTTAAATCCAAATTAAAAGATTTCTCAATTCATTTAATTTCACTTTCTATTTTAACTTCATTTTCTCATAGTTCTTTTTTATATAGAAAATCATTTTCAGAATCATTTTTCTCAAAAAAACAATAAACATCTGAAATTTTATTACCTTTAAAATTAATTACATATTTATGAAGATTTTGAACTTCTTTTTCTAAAAAAAATTCAATGCAAATAGAAAACAAATCGGACTTTAATTCCTTATAAAATTTTTTTCTAAATTTTTTTGATTCATTATATTTATCTATTAAATCAAATTTGTTTTCTATACGAGATTTAAAATTTTGATTTTTTTGGAATAATTCTGTATAAAAAGTTGGACAAAAAACTATTATGAATTGTTTTATTGGGTTATTATTTTTATTTCTATATTCATATAATTTAAAATCACAAATTTGTGCAATATAGTCATAATTATTTAATATTTTTAAAATTTCTAACATTTTGTTTTTTACAACTTTTCAAAATTTGTAAAAATAAATAATATTAAAAAATCATTGTTTTTCTCATAAAGAATAATCATAAAAACTAAATGGATCTAATTCATCATTTTTTTTGGTTAAATCTAATATGGTATCTGAAAAGGAGCAAAAAAATAATTTTTGATCTATAAAAGTTGCTGAAATATATAAAAAACTATTATCTGTTGAAGTCGCAGATGAATACATTCTATAAACTTCACTTACAGCATCAAAAGACTTTTTTATTAAAAATTTTTTGATTGTTTTATACAAAGAATAAAAAACAAAAGTTATTATAGCTAATACAAAAGTTAAAATATTTCAAATCATATATCCTCTTTTTTCTTGTTTATATTTTACATATATTTTTAACAAAAGATAATTTATTTTAATGAATTAGAATAAATCAAAATATCTATTTTATAGCAACAAAAGCACCAACTGGTTAGCCCACTAACCAGTTCATTTTTTTCTTATTTAATTCAAAAAAGATAAAATAAAGATATGGAAAATTCAATAGATCAAAAAAGTAAAATAAAAGCTTATAACTTATTTAACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCTGAAAGTAATTTGGAGCAAATAATAGATAAGTATATTGAAATGAATATAGCACATCCATTTCTAGAAGGCAATGGCCGAAGTACTAGAATCTGATTAGACTTAATGCTAAAAAAGAATTTACAAAAAGTTGTTGATTGAAGTTTAATTGATAAATTTGATTATTTACAAGCTATGCAAGAAAGTCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGAATTGATTATTCTTACTACTATGAAGAGTAATCTTTTTTTATACTTCTATTTATTTAAAAGACTTGTTTTATAGTCTTTTTAGTAAAAAATCAACTATTAAATAGTATCTAAATACATTTGATTATTTATGTCTTCTAAAGGTTCTCTAATTTCCTTTTTAGGTACTAGTCAATGGCTTGAAAATAAGAAATTTAAAATAGTTAAATTTATAATTCCAGCAACTATTATTCAGTCCATTCTAATTTCAACATTTGAATAATCATCAAATTTGAAATTATTGTCAACTAAATAATTAATTTGGTCATATTTGTATCATAAATCTCGATAAAAAGGATTGCCATAAACAAAGTAAATTAATAATGACTCATCAGTAAATTCACTAAGATAAAACAACATATCATGTCAAGGCTTACCACCTAAAGAACTTTTAGATATATCTTCATTTCTAACTAAATCACATAAATTAGCAAGAATCATATTATTTATATCCATTAAATATTTATATAATCTGGCATATTTAATATTTGCTAAATTACCTTTTAGTTTATATCTATAGTCACTTTTGTCGCCTACTTCTTTATTTTTAATTGCTTCTTTAACTTTGTCAAATAATTCCTTAGGTTTAATTTCTTTCATACAATTAATTGTTTGAGTGTAATACTTTTTAAAGTTTTTGATGAAGAATTCAGTATTAAATTCATTACATTTAGCTTCTTTATTACTATCAAATTTATTAAGAACTGCTCATTTTGTATGACTATATTCAATTTTGTCTAATGTGTTTTCTTCTAATTTCAAGTAAGAATTTAAAAGCTTTTTTATTTCTGAATTAGCAATTTGTCCACTATTAAATTTAGTTTCACTTTTTTTGTGTTGATCATAATATCAAGAACCAAATTCTTTTAAATCTTCTTCATAAAAAGTGTAATCTAAAAATAATTGTTCTGTTCATTCTGGAATTAAATGAATTTCAACATCCTTGTCACTAATTACATTAACACGTGGAATATCTATTTGAAAGTTTTTTCTTCCTAAATCAGCTTTTAAAGTTTTTTCATTATTTAGAAATTCAATAAAGTCTTCTCTGATAGTTAAACTATTTGCTCAATTAAGATATCAAATACTTTTACCTTTTGAATCACAAAGTTGATAAGCCACTACTTTAGGTGTTTTTTTGTTGCCTATTTCGCGTTTTATATAATTTCTAACTTCTTCATACATTTCTAATTCATATTCTTCCATGATGTCGCAACCAACTTCATCATTAATGTCATCTGAAGTTATAATATTAAATAGTTTTTTCATAATTTACCTCTCTTTTTATATAAATTTTAAATAAATTGCAAAATATTTTTTAGAAAAATACATAAATAATCAAAATTTAAAAAACAAAAATTTAGATTTTGGATTATTTTAATTTGATAAAATAAAATTATGAAAAGAATAAAGATATTTGACAAAAAATTAGTAGAAAATTATAAAGATACATATTCTTATTTAATTAAATGAATTGAAGAAAATCCGCATGAATTTTTAGCTTGCTCTCAACATCAAATTACAGAAAAGATTTATGTATCTCAATCTTCATTATCTAGATTTACAAAATTTTTAGGTTTTGAATCATATCGTGAATTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAATTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCTAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATGAATTCGATCCATGATTTTAAAGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGACAAAAGCAATTTTAATTTTCAAATATTGACAAGAAATGAAAATGTTTCTTATGCAAATTGCACCTCACCGATAATTTATGATTATATTCCAGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTTATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAATTAGTGAATAAAAAATTCAAAAATAATGAAAAAATTCGGTTTTTTTCATATTTTTTTGCATTTTTTGTCATTTTATATTTCATTATTGAAATTAAATTCCTAGAAAAAAGAGGTCAATATATATATTGATAGAATAAAGTTGAGGAGGGCAAGTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTGAATAATAATTACAACCGTAATTTTGCTTGACTTTCATTAGCTATTTTTGCAATCGGATGAGTTTTGTGAGAATTCGCTATAGCTAAAAGTGGTAATAAAAATAAAGCTTATGCAGCTATTGCTTTTATAGTTGTTGGAAGCGCTTTAAGTTTAAATATCATTAACTATATTTGCACTTTTATTGCATTAATCGTAATTGTGCTTTTAACAATTATGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGTTGATTTAGAAGACAACAAGAAAAAAATACAAAAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGATGGAATTTAAGGAGATATTATGAGATTTAAAACAACATATGCAGTTTCAGCAAACGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGTGGAAAACTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACACCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTTTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTCAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGTCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAGGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGCTTACAATTTTAGAACCTAACAATATGTGAAACACAATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGCCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGAAGTATTCACTCAGGAGTAGGAACAAGTAACTATGCTTTTATTTGAGCAATGGGTGGAGAAAATCTTGATTATACAGATATGCAAGGTGTTAAAACAACAGATCTAAAATAGTGTTTAGAAATCTAAATAATTCATTAGTCTATGAAAAAGAATTCAACGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGAACAGATGGATTTTGAACAGGAATTCTTTGATTAGCTTATGAATACACTAATAATAATAAATATAAAGAATTAGCACTTGAGCATGTCAAATGATACAAAAAAAGAATTAAAGAAAAAATATTGGTTGATCATCATGACATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGAGGAAATATTGGGGCAAATGATAATTATCGTTTAATTATTGACTGCTTATTAAATATTCCTTTATTATATTGAGCTTCTGATTATGATAGAAAAAATTATTTCGATAAAGCATTAAATCATTTTAATACTTCTTTGCATACTGTTATAAGAGAAGATGGAACAACATATCATACATACTTTTTTGATGCTAAAACAAACAAACCTTTATGTGGTAAAACTAGACAAGGTTATAGTGATGACTCTTGCTGAGCAAGAGGACAAGCGTGAGGTATATATGGAATTCCACTTACATTAAAATATGCAAAAAATAATTCAAAAATAGATAAAAACGCTTATCAAATTTATCAAAAAGTGTTAAATCAATTTTTAAATAATTTGCCTCAAGATGATTATGTTTGTTACTGAGATTTAATTTTTAATGAAAAGGCAGATCAATCACGCGACTCATCAGCTAGTGCTATAGCAGTTTGTGGTATTTTAGAAATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGACATTCAAAAAAAGGTGTTGATGAAGGAAATATTTGAGGTGATTATTATTACTTTGAAGCACTAATGAGACTATATAAAAAGGATTGAAATCCTTATTGATAAAAGGGAGATTTTATGGCAAAAATTGTTCAATGTAGAGTTGATGAAAGATTGATGCATGGACAAGCATCTATTTGAATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGACCTAAAGCTGCTGATTGACAATTAATTTACATTGTTGTTAAAAGTATCGATGATGTTTATAAATTAGCTTTAGGAAAATTACCAATTGAAGAAATAAATATTGGTAATGTTCATACAAGAGAAGATCGTGTAAGAATTACACCTTCAGTTAATCTAAGTAAAGATGAAAAAGCAAAAATTAAAGAAATGATTGATTTAGGAATTAAGTTTAATACTGCAAGCCTTCCTGGTGTAACTAAAGGTTTAGTTGACCAAAATAAATTAATTAGTTAGGTAGGACAAAATGGAAATTTCAATTATTCAAGCTATCTTTATCGGAGTTTGAGCCGCGGTAGCAATAACAGGTACTATGATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGATTAGGCTTTGGTATTTCACAAGGTGGTGTTAGACCGCCAGATCCAATTGCACCAGGTATTTTTGCCACAGTTGTTGCAATTGTTGAAAAACACGGAAGCGCACAACCTATTTCAATTGGGGATGCTGGTATTTATATTGGATATTCAATTCCAGTGGGAATCTTAATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGATTAAGAACCGGATTAAGAGTTGCTGGTGGTATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAATAACAAAGATTTTGTGCAAGTTAATCAAGCTCAAACTAAAAAGCTTTCAATTTGAACTTACATTATTGTTGCTTTAAGAAGTTATTTTCTTCAATCAGGCTTTAACTATGCTAACTTTCAAGGTTTAGGCTATGCCAACATAATTTATCCTGCTTTAAAGAAAATTTATGGTTATGGAACTCAACAATTCAAAGATGCTTTACTAAATAATATAGAATTCTATAATACTAACCCTCAATGTATTCCGCTTATAACTTCAATTCACATTCAACTTTTAAACAATGGGACAAGTTGAGCAGATGCAAGAACTATAAAAATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATCGGTTTTGCTCAAAGCGGAAATATACTGGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATTAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTCTAGACAACATTATGCCACTATTTGCTTCAGGAATATGAGTTATCTTTATTTACTTTATGGTAACTAAATATAAATGAAGTCCATATAAAGTTATTGTAGTAACCATTTTAGTTGGTGTTACTGCTGGAGTATTTGGAATTTTAGGTGTTTAATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGGTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAATTATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGATGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAATGGGAGATTATCATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAAGACAAAGTTGTTATTGTTACAGGTGCAAACATGGGATTAGGCTTAGCTTATGCTAAAGCTTTATCAAATTGTGGTGCCAAATTAGTTATTAGTCATTTTGACACAAATACGAAAGAAATAGAAAACTTTTGTGCTAAAAATAAAACAGAACTATTGCTTGTTCAAGGTGACTTAACTAAAGAAACAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGTACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGAAATAAAGTTATTGATATAAATATGAATGCTGTTTGATTCTTAAGTCAAAAAGTAGCTCAAATAATGAAAAAACAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGAGCAAAACCAGAAGAAATTGCCGGTGCTGTTGTATTCTTGTGTTCTAAAGCAAGTGACTACATCTCAGGAGCAATAATTCCTGTTGATGGTGGTTTCTTATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGACTAAAATCAGGAGCTAAAGCAATAAGTGTTGGCTCTAACTTGTTAGCTGGACAAAGTGAAAAAGAAAAACTTGAAAGTATTAAGAAATATTTAAAAATAGTAAATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGAAATAACAAATATGCTGATTTTAAAAAAGAAATTGATCCATTTTTAAATTTGTCTGATATTGTTTTTGGTTGAATTTCAGAACAAAATTATTGCAAAAGTAATAGTTCAATAAGTGCAAAAGATTTTGACAAATTAACCTGTTTAATAGATAAAAAATACAAAAATATCGAATTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTGAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAACTTGAAAGGTAAATATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATAGACCATCCTCTTGCTAACTTATATTATGCTGGAACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAATAAAAGCTAAAAAAACTTTTGATCGAGAGTTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGCCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCCGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGAGAAATTAAAAAATATTATTGAGAATGTTTTAGACGATTTTGCAAAAAATTATTATTACAACGGACAACAACAATATGTTAACTGATGATTTTATGAAATTGGTATTCCTAAGGATTTAACAAAATTATTAGCAGTTTTAAATAACGTTTTTACTAAGGAAAAAATAGAAAAATGAAAAGCTGGTGTTGATTACTTTTTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAACCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGATGGTTTTTATGTTGACGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTTCTGTATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCTTAGAAATAAGGAAGATTGGGAATTTTATGAAAATAATTATTTTGATCAAAGAAAAGAACCAAGTTCAACAAACGAGCATTCAGAAAGCCCAGACAATGCTTTAGTCGGTGTTGATTTACATAAAGAAAACGATGGTTTAGTATTTACAAAAAATCAAGATAGATATGTTTACAAACATGATGATTTTATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTCCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGACAAGCAGTTAATACATTCAAAATTCCTGGCACAACTTCATACATCTCCTCTGCTTACGATAATATATATGATTATATTTTTCCATCTTATATGCCAGTCGATATTGAAAAATGAAAAGAAATAGGTGAGGTCGAAATCAAAGATAAAGAAGGAAATCCAATTCTTGATGCAAATGGTAAACCAGTTAAAAAAACAGGCAAAGAACTTTATGACGAGTATCAAAAATTTATTAAAGAAGATTTGAAACAAAAAGAAAGAGACATTGAATTTCAAGTTTCAACAAAAACTAATAAATCATTTAATAATGGAATAATTTGAAACAAAATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACAATTGAAAATAGAATGATCATTGATAAAAGCACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAGAGGAACAAGATTTATTTATTTGAAAAAATAAATTAAATGAACAAAATGGATATTTCATATTAGGAAATAATAAGGCAAAAGTTGAATATTTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGATAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTTATAGCGTTAAAATATCAAAATAAAGACTCTTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATACGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCCGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGACTTAGATCTAAAAATTAATTTATGAAACCCTACTACTATGATTATCAAGAAAGTTCAAAATTCTTCAATCTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTGTCAATTTAAACGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGATTTACATTAACAAATTAAAGGAGTTATTATGAAAAAACATAAAATTATATTTGCTTTAACTCCGTTCACTTTCACGCCTTTAACCGCAATAAGTTGTAGTATTGAAAGTTCAAATGATTATGAAAACGCTAATAAATGATCTAAAGAATCAAACAAGCCTGGAATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGCGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTAGTTTAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTCAGATGAAACAACTACAAATTTTGACCAAAATTCCTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGAAATAAAGGTGTGCAATATTTGCCTTCATGAATACAATATGATGTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAGCTAACATTATACAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAGTTAGAAAGGAAACAATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATTCTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGAGATGAAGAAAAAGGAAAAGTTTCTTTTGAAATAGCAAATCGAAAAAGAAAACCAAGTACAGTAATAGAAAATGAAATCGAAATAAAAAGACCTCAATTAGATTCTTCAATAAAAATTAATTTAACTTTAGAACCAAATGATGCTGAGAAAAAATTAGTTATTAAATGAAAAATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGATTTAATAAATCAATTAAACTTCTTGAAGCTGAAAGTAAAACTAATTCACATATTGAAGATATATATCCAGCTGTTCAATCACTTAATTCAATTTTAGCTTCTTATGATCAATTAGAAAATGATGAAAAAACTAAAGCTGATGAAATTATTCCTGCAACAATAAATGCTTTAGCTGAAAAATATTTTGGAGCTAACCAAGTTATTTATAAAGAACATTATTTCTATCAAGAAAAAATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGAGCTAATAAAATGTATTCAATAACTGTTTCTAAATATGTTATAGCTTTAGCTAATAAAGATAAAGACGAAGCAAAAAACGTTCTAAAAGATTTTTGAGAATGAACAAAAGATAAAAATGCAGCAAATATTTCATTAAAAGAAAAATCAAGTTATTACTTTAATTTTGACAATAAAGACAATAACAAGAGATTGTATAATTTTTCAAAACAATTCGATATGCTTAGAAATATCAATAGATTGTTAACTAAAAATGTTAAGAATACTGAATGAGGTACATTATTTGATTCTATTAACAATTACATTAATAATGAATTAATTGACTGAATCAAAGATACTTCAAGCTTAGTTAAACTTTCAACAGGAATTCAATTAATTGGCTCATTTATTAAATTCTTAGAAACTGAAAAAGATAATGTAAGATTAGAAACACTAAAAACTAATCTATTAGCAAAAGAATTAAAAAATATAACAGGCTATGATCAAAAACAAGGCGGTTGATCATGAGCTTGAGATGCAGTACATAATTTACTTGCTTAAAATATTTTAATATCACATTCATTTGTGATATTTTTTTAAACTTTTATTATAAAAAACCAGCCTTTACTAAAAGCTGGATCATAATATTAAAAACTAACTAATTTTCTTTTTAACTAATTCAAAATATACACGAATAATTTTCGATAGTATTCTCTTTTACTACTGCTTAAGTCTGAAGGTAAGTTATTAGTTGCTTTTTGAGTTATGCTTTCATATATTTTTTCTTGTTCTAATATTTTTTATCAATACAACTTAGAATTGAGAATCAATCCTCCATACTTAAACTTTGAGCATAGATTTCACTTGGTTTTTTTGTTATTTTTAAAGTTCTTTCTTTTTGTTTAATTTACTAAAAATATATTAGTTTATAAAACTAAAAAATAAAAACTTAATTAAATTTTTTAGTAAGTAATCACAGAAATTACTGCATTATTTCTTAACTTTAATTTATAATTAAATAACTAAAAAATAATAAGGATGGTCCAAATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGAGGTGTTAGAATTGATACTTCTAAAAATATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAGAAAATAATTTGGTGATGTAAATTATTAATGGGATAAGTTAGAAATCTAAAAGAGAAAACGGTATTTCCTTTTTCTATCAACTATATTTTATATTAAAAAATGTAAATTACAAAGAAAATTGTATGGAAAAAATAGCAAATTTAATTGCCTTTCTATACTAATATTAATATAAATACTAATATGTCTGAATAAGAGTATTTTGAATTTTATTATCATTCAACAATAAAGCTTCTATTGTTTTTTGAGAATATAATGCAAAGCTTTTGTTCAGTTTTCTTTTAATATAAACAGATATGTGTCCTTCTATTAGTGAACTATAATCATTATTAATGATTCCACTTTCAAGTTTAGATGTGTATCTTATCAAACTATTTAAATTGTATCTATCAATATCACTCAAAACAAAAGAGTCTCTAAAATAGAGATAAATTGTATTTAATATATCTAACAACTTTCCATAATCTCTACTGTTGTAAGCTTCTGATATTTCTTTTTCGATTTTTCACCCACTTAAATTTTGAATGCTATCTATAAAATTGTTAAATCCACGATTTCTCTTTTTAAAAATAAAATCATTAAATTTTCTTAAAAAGTGAAATTTACACAAAATAAAATTGCCTTTTAATTGTTTTGCTAGTGTTTGAATTCAATGAGCACCATCCCCTAAAATATAAACATTAAAGCTATTATAATCTCCATAATTTTTTGTTATTGCTTCTTTAATTGTTTTTGATAAACATTTGCTGGAAATCATTTTAGAAGATGGCAAAATATTCATTATTTTATTCAAATTAACTATTTTATTATTCTTCAATTGATATATTGTTAAAAATCTTATTTTTAGTTTTTGTTTTGTTCTTCCTTGATTTGAATTTATAAAGGTATCATCGATACCTATAAAAATGTTATTGTTTACTAATTCATTCTTGTAGTTCTTTAGACTGTTTATTTTATCTAAATTTATTTGTTTTACAATTTTGTATATTTGAGTTTTGCTTACATTGTATTTGCTTGCAAGTCTAGAATAAGTGATTCTATCTTTAACAGCATAATTTTGAATTCTTTCCTTTTCATCTTCACATATAGATGATCCAAAATTTAAATAATCAGGATAAAATGTATGCAACTTATTTGTTGTTTTATCTTTATATCTTTCTACTTTCAATTTAAATGTAAAATTTTCAAATCTCACAATTCTTACAATCACATCATGAGCTTTATATTTACTTTTTCTTTCATCACTATATTTATAATCAATTAATTGATTAATCATATTTTTGATTAAGTTAACAGCAATGAATTTATTTAATGAATTTTCATCATTCATAATTAATGAACTTAATGAAAAATTCAAATTACTTTCATAATAATTTTGTTTATCTTGTAAATTCATATTTTTATTATAATCCTGTTTTATAGTATTTTTAGTAAAAGCATAGAAATAGAAAAAGAAGCCTATAGGCTTAATCTATAAACGTTTTTTCTTCTTTCTATACCATATTGCAATAATTGTTGCAATAATTCCAACAACACTTAAAATAATTACTGGAATTAGTCATGAAAGTAATTTTTTCTTGTTTTTAGATTTAATTTCTAAAGCTTCTTTTAACATTTCTTCTGTATATTTATTAGATAAATTGAAGTTTTTATTACCACTAAAGTCATTAAAACCTTTCTTAGGTATTATTTTGACATTTTTAGTATGTGTGCCTGCTTCAATAAAAAAGAAATTTCAATTACCATCTTCAACAATTAATTCTGTCTTGTTTTTTAATTCTTCTTTTGGAATAAGAGTATTTTTTTCTATTATTTTATAAACAGAATCAATAAGTTTTTCCTTGTTTTCTTCACTAGTTGTAACAGTATCAATTTCTAGTTTATTTAAGTCATATGGTTTAGTTGCTTTTAAATTTTTAACAACTATCTGAAAGTCAACGCCATTATCATCTTGCAACGTGTAATGTAAGCCTTTAAGAATTAAATTTGTACCTATTTTATATTCTAGGTTTTTAAAAAATTCATCTGTTCATAAAAAATCATATAAATTATTTTGTACAAGCTTTGAATTTGGGCCAAAAGCTTCTTTAATTTTATATGTAATTTGTTCTTTTGCTTCTTTCTTAATTTTTTCAAAATTTTTGTCCATATCAGTCGTATCAACACTTATTTCTATTTTTTTTACATCAGGCCATTTAAATTTACCTGGTGGTGCATCATTAATAAGTTTGATATAAATGCCATCGCTAATCTTTCTGCCTTTGATTTTGAACTTTAATTTAGCTTTGTTTTTGTCATCTTCTCAATATTTACTATCTTTTCTAGGTTCTAAAACTTTTATTAATTCATCTTGGTCATATTCGATAATTATGTCGGACATCATAATATTTTTTCATTTAGTTTTTTTGTTTGAAAAAAGTTTGTTGTTTTCAGATAGTTTTTTGCGATTATATTCATTTAAATAACTTTGTAAGACACCATACTCGCTTGTGTTTTTAAGATAATTTTTAATTAAATCAATCGTATTCCGGCCATAAAGATTAATAACAGGTGGTTCAAAGTCAGACAAATATGCTTCGTCAAATGCTCCTAAATCAACGTCATACTCTTTTTCTGAGAAATTACTTATATAATATTCATTATTTAACATACTAATATTAATTTTTAGTTTTTTATGGGTATCTATAATATATCTATACTTAATTATTTTATTAATATCTTTGATATCTTCACCCGTTTCTGAATTTTTAAGAGATTTAACATATTTGTCGACAGTTAATGGTTTTATAATATCTTCAAATTCGTTTATTGTCTTTGTCTTATTGTTGTTTATAGTTTCAACATCATCGTTAAATTCTAACAATAATGCTATTTTAGATAAATCAATTCTAAAGACTTTGTTATTTAGGTTATAGTTTTTATCTTTATGGTCAATATAGATATAAATGCTACCATTATCACTTTTTCATTTATTTTTAATTTTTAGATCATTTTTATCTATTTTAGATGTCGCTTTAACATATTTATTTAAAAAATCTTGTTCATCAAAATTAGAAGCTTCTAAATGATTGAGAAGTTTTTCATTAGCACTCTTATTTTTATCAGGTTTAAAATCATTCTTTAGTTTTAGAATATCTAGTTTTATATTTATATTAAATGTAGCAAACTTATATCTATAAAACAAGAAGTCTTGATAAAGAGGAAATAACTTATCATAAGTATATTTATTCGAATTTAATAAATCTTCTTCTTTAAAATTACTTGTGTTAATAACGCCAACTTTAATTAAGTATTTTTTAAATTCTTCTGATATTTCAAAGTTATTTGATCCTCAGAATTTATGAACAAAACTTGGTAGGCCTTCTATTTCACTTAATCTTCCTTTATTAGCTTCAATTTCAAACTTATTATCTATTCATACTAATTTAAATTTACTTAAATTAGATTTAGAAATATCATAAAGCAAGTAAAATCCATTCTCTCTGATTTCAAAATCTGTTTTACTAAAACCTTTGGTGAAGTCGGCATCTGCTGAAAGCTTCCCATTATCATATTTTGCTTTTACAACTATTGTATTAGCATTTACTTTTATTGCACTTGTTTTAATGAATTCTGCTGGGAAGCCAGAAATAGGGGTGAGTTGATTATCTATGTATGTAAGATTTTTAAAGGTTTTTTGTATTCATTTTTCAGTCTTCTGCATATGAATTGATATATTGCTTTCATTTGTTGTATCTATTAAACTATTTTTAAAAGTAAAAACAGGTAATTCAGTTTTTCATCAGCCTATTTTTTTTCATACTTCATGATTTGCTTTTAAAATTTCTTTATTTTTATCTTGAAAATCTTTCATATTTTTATCTCAAAATTTTTTAACTTTTTTGTAGTCTTCCATTTCAAAGGATTTAATTCCAAAATTTATAAATTCTTTTAAGAAAAATATATGGTAAAGAGGGCCACCTTCTTTCTCCATTTCTTTATATAGTTCTGGGTGCAAACCAAGACTATCCTCAAAGAATTTTTTATTATCGTAATATTCAAAATCAACAATTCTATAATAATAATAGTAAAGAATATATTTTGATAATTCATGCAATAATTCATGCAAGAATTCTGAATAATCGTAAGAATCAAAACTAACATATACACCTTGCGAAGATCCAGAAATACTTATAAAAAAAGGTTTATATTCATGTCTGAAACAAGATGAAAGAAAAGAATACATATCGCCATGTTTAGATTTTTCGTCAAGATTATTAAATAAATTTTTAAGTAATTTTTTAAGTTCCTCGATTTTTAAATAAATATATTGAAATTTTCGTAAATAATCATCGCTGTTTCAATGAATATTAAACGAATTATCAGATAAATATTTGTCTACTTTTGTCCCATTAGAAAAAAGACTATTATAAAAATTGTTATATAAAGGCAAAAAATTTTCAAATGCTTTTTTATTCTCAAAACCTTTTTTTATTATATATTCCCATACACTATCAATTCCTGGGGTGTGTAAAGAGCTTTTGTCTGAGTAAGGTGTAGGAGTATTACAAAATAATAATTCAAAAAGCATATTAATTTTTTTCTTTATGTAATCTTGGTCAAACTTAATATCTTCCCTTTTCAATTTATCTCATAAAGGATGATAGAATGTGGTAATATCAAGTTCTGGATATATATTTTTTAAATCCACCCACACCAATTCACTTTTTAATCCTGTTTCAATATTAACTAGAGAATCATAATTTTTATTAACTTCGTCTGTCTCATTACCTTCTTTATCAAGAATTTTAGGACTAGTTAATTTTTTTGCTAAATCTGAGTTCTTATCTCATCCAGGAAATGATAAATTTATATCAGTATATTTTGAGGAATTATTAGATTTAAATGTAATAGTTTCTTCTGATGAATTAGATTTACTTTTATACTTAATTTTTAATTCTAAGCCCTCATTTTTGTTATTTTTATTGTTTTTAGTGGCTGCTAAATCGAAGTGTAATATTCCATTACCAGCTTGAATTGTGTTAAGACCTGAAATGGTAGAACCATTAAATGAAATTACAAATGTTTCATTTAAATCATCAGGTGAGGCAATATAAATATTATCTGGTATATACTCTATATTTTGTGTACTATCATTAGTAATTTTTAGATTAGTTTTATTAAAAACAATTTTGCCATTTTCGGTTTTATAGTTAAATCCAGCGTCGAAATAAATTCTTTCTTTTCAAGATAAGTTTTTAAAGTCACTTGGTCAGTTTCAATTAATTAATTGATTTTTAATTAAATAGTATTTCTTATATTGGTTATTACTTGAGTTAAATTCCTTTAAGATAATATTAATATACTTATCATCTTTAATTTCAAAATCAAAGTTATATTCAACATCATTTTTTAAATTTAATTTATTTTTATCAAAAAATGTGTTTAGTTGTAAATTTTTAAAATAATCATCTCAAACTTGTTTTAAACTTTTATTATTTTTATAGTGTTCTAGTTTAATATCTAAATTTAAATTGTCTTTAAATCCTGTGGTTGAATATAATGTAATTGATTGATTTCTTTTTAAACCTTTGCTTTCAATAAAATTAAAAACAACATCTCATTGGAGTGGTTTTCCTTCTTTATCAACAAAAGGAGAATTCTCCTTTAATTCTTCATCGTTTCAATAATTAACAGTGATTTTCTTTGTTATATTAGTTTCTTTATTAACATTATTATTTTGTGTATTAACAGAGTTTGAAATGAAAATAGTACTTAATGGGACAAACCCACTAAGCACTGCTAAAAGACATATTTTTTTGTGTTTTTTCATATTCCTCCTTTTTATAATTTATCTGTTTTATAGATAAATAAGTAAATTTTTATATATTACTTAAATCGCCTGACTTAAAGATAATTTCCTTTTCTATTGCATTGTAATGTAGTGAAATTTGAAATCTTGTTTTGTTGTCGATTGAAAACATTCCACAACCAATTGGTGCTGATGAGAGAAAGTTTTTTTCTCAATAAGTTAACTCATTTTTAGATTTATACATTTGACTAATAGCATTAACACCATTTTGATTTTGTCTTAAAATAAGTGTATATTGTATATTTTCAACCATACCTTTTGTAAGGTTCATAACGTCTTCACTTAATTCAAAGTCACTTGGGTTTTGTGTAGTTAAAATAACAGAACCGTTGTATTTACGAATTGTTTTAACTGTGTCAAATAAGAATTCTAATGAAGACATATTCTTAGCATTAATAAATCTATGTGCTTCATCAACAATTAAACAAATCTTCTTAGTTTTGTTAATAAAGAAGTTGTTTGATATTCTATTTCTAACGTGTGAAAGAATCAAGAATAATGATGCTTGTCCTTCGTTTGAAGAAATATTATTAATTAAGTTATATGTATCAATAACAGTAAATGAATTATCAAAATCAATATTTGTATGTCCAGTATATAATGCACTAAATATTCCATTATTTAAGAAATCTTGATTAAAGATTATAAATAGTTTATTAATTGAATCTTTGTAATATAACTCTGTCTTCTTATCGCTAAATTTATATTTACCTAAGAAATTAACATAATCAATCATTGTTGGGAATTCATTATTTTTCATCTTTTCAATAGGTTTGAATTTTTGCTTAGCATAAAACTCTGATAGTGTGAAAGTCACAACTCTTAAATCCTTTTCATCTAAGTCAGGATACAATAATGAAAAGAATTCTTTAGTTTTCTTAATATGGTTAGTTATTAACATATTGTTTGTAACAGCAACATTATCCTTTTCACTTTCAAAGTTAACTTGTAAAGGATTTATTTTGGTTTCAAGTCCTTTGCCCATTTCAATTCAGTTTCCATTTAATTTATGGCATAAATCCTTATATTCACGTTGTGGGTCAATAATTATGACTTCACTACCGTTAACAAATTGATGTAATGCAAATTTAGATGTAGCAGCTGACTTACCAGCACCAGGCACACCTCATAAAATCATTGAAAACGATGTTCTGTCCTTTGTTTTCTTAAATAAGTCAAAGATAATTGGACTATAAGAGTTGGCTGTAAAGCCTAATAGAAAGTTATTACCATCATTCAATGAGTTTGATACTCATGGTCAACCATGAACAATATTACGCGAGCTCATTTCAATGCCATTTCTCAAAAAGTCTTCTTTGATAAATATTGAGTTTTGTAAAGCTTCTTTTTGTAAATAGTGATTTAAATAAACATTGGCAAATGCTTTTTTAGCGTTTATTCCATTCCGAACTTCAATTTCGTGTAATGTTTTCAAATCTTTACTTTGATTAACAAAAATTAAATTTGAATCAAATAAGTTCATTTTTTGAACGTTAATAAAATTAACTAGTTCGTCCAGTACTTCTTGTTCAACTCTAATTTTTTGAATTTTAAAATTACTTTTTGTGTCTGCATTAGCATTATTTAATTGCATAGCTTTATCTAGTATCTTTTGTTTAGTTGCTTCATTAATTGGTTTTAAATTCCATACAATAAGCGATTCACTGCTAAATAAGTCACATGCTCAACCTTCATTAATTTTTAAAGAACTAAAATCATATAAGGTTTGAAATGATAAATATTTTTCATTTTCTCCTTTATTAATTGTTATATGCTTTGAACCGAACTCTATTGTTTCAGGTGCAAGCAAGTTCATAAGCTTATATTCTTTATCTTTACGTAATTTCTTTAATTCCTTGTCTGAAAGTTCTTTGCCTTTCATTTTAGCATCTAATATCATAGTTTGCTCTTCATCAAGTGTTTCGTCAAAGAAATTTTGTTTTTGTTTTTGTGATAATGATACATTGTAAATCATATTTGTAAAGTCTAATAGTTCATAGCCGGTGACATTTGATAAAGGTATTTTGGTATTTTCAAATGACTTGATAAACTCATTTTCTAGTTGTTTAATTTCATTTATATTCTTACCATAAATAACAAGATAATAAATATCAATGTTTTCTATTTTTTTAAGTTGTTCAAAGTCATGTGTATTGTAAATAAAATGGTTTTGAACAAGTTCGTTGCTTTGTAACATTTTGCTTTCAAAATACTTATCAACATTGTATTTTAAGTTATCTTCTATATTCATAGAAGCTGGCTTTTTAACAAATGTGAATTTGGTTTCAATTGAGTTAAATACATTAATTAATTGATTAAAAAATGCGTATTTATCATCTCTGTTGTTTGAGTAAATATTAAAACCATTAAATTTAAACACAGTAAACATACTTGTTCCAGCTGATGATAATGTTTCAACATATTTACTTTCAATTATTCTCTTAAATGGTACAAGTAGTTTTGTATCATTTGGTGTATTCTTTTTATATTTCTTCGTAGAGGATTTATATTTAAATATTCTAACTATTAACGTATAGAGCCTGCAATTGTGTTTTTTACTTGGTATTATCAATCAAATTAACGATAAGAATGATACGGCAAAAATTACAACTTGAACTCAAGTGTTAAGTTTATTAGCAAATCCTAATGCTGCTGAAAAGCCTATTATTGCAAATAACAACAAAATATCATAAAGGTTTAAATTCTTTACGATATTAAGCTTTAATGAGCTTATTTTCTTATTTTGATTCATGTTTTTTTGTCTCCTTTATGCTATCTTTATAGTTATCTATTTCTTGATGTTTTACAAGTTCTTGGGATGCTTTTTTCATTTCAGCAAGATTTTTCACTATATCTTTTGATGAAAGAGAAATATCGCTTAATTTTTGTTGAATATCCCCATTTTTATAAATTGAAAGATCAGGTATTCCCCTTATTCCTGCTACGCCTTCGATATTTTCTTTGGTTTTCTGGATATCTGCTGGCGGAATTTTCTCAGTGCTTATTGCTTTCTTATTGGTCTCGTCTTTAACAGAATAAGCTGTTGCATCACTCGTATTTGCATTTTCATCATTTCTTTTTCCATCAGAGGAACCTTCACTTTCATTATCAGCAGAATCTCCTCCTCCATCAGCACCAGCATTTTTCTTTTTGAGTTTGTTCTTCAATTTTTGTACTGGGTGTAGGTTTTTAACTTTATTAACTGCGTTTGAAACAAAGTTAGTCATTTCTACTCCAAAGATTGAACAAACTATTTTTAATAGTGAATTAGTCGAATATAATGCGGCAACAAAAACTATAAGTGCTATTAATCAAGGTCATACTTTAGTTGCGTTAGCCATAGGCATTATAACTTTTGAGCCGGCTGAAAAAAACATAACCAGTACTGACAATGATAAATTCAAGACCATAATGGAAAGAATCTTGGACACAAATTGTTGCTTCCATATATTAAGCTTCTTGCCACCATCTTCAATTGAGACTACTGCTATTAATGGTGATTGTATAAATAAAAAGAATGCTTGATATGAGTTGATTGTTATATTTATAGCAATATTTGCAATAACTATCATTGACCAAATCAGTACTAATGCATTTATCATTAATACAACGCTATTTCTATTAAGTTCATTTTGTGAAGCAGAGTATTGGCCATATGTTGGTTCTGTTGGATTTGGAAATCTTGCCTCTGGTTGATTTATCGCTTTATAAATTATTTCCCCTAAACCTGCAGCTGGGTCTGCCCCTAAAGCAAGATTTAGCATATTAAATGCAAATTTTGCAAAAAATGCAAGGACCCAAACAACAATTGGAATTAAGATTATAAAAGCCATATGTGGTAATGCCGCTTTGAAACTCTGCTTCATCTTTTGCATTTTGTCTTCTCCGCCGCCACCAAAAAGGACTTTAAAACAAGCAATACCAAGGACTATTGCAATCATAACAAAAGCAAAAAGTGCTAGCCCTGCAAAAGCCATTGGTATGCTTTTTATATCATCTATTGATTTAATATCATCTATTGATTTACCGAAAATTAATAATGATATAAAATCAAATGATAAAAAGTTAATCAACTTTCCTAAACTATTAATTATTGTGAATGGAAAAGCAACAAAAGGAAATCAACCAATTTTAAAAAAGAAAAATCCTATTCTTTTCAGCATATTTTATTATCCTTTAAGTGCTTCGCCACCATAATTAGTTTGAATTATTTTTAAAATTGATGGTGTTAGAATAAGAAGCACAAGAAGTACAGCAGTTGCAATTAAACAGCTTTTTAATACAACTTTTAATTTTTCTTGGTAAGAATCATCTTTTTTGCCAAAAGTTGCCTTATGACTAACGATTATAATAGTAATAATAACAGCTAAGGCAAATAAGGAGCCAAGTATAATACTTGACCAAGTAATAGCTTGGCCTATCAATGCATATTGTATATTTTTAAATGTTGTTTCAGCTGTTGTACTTGTATCTACATTGCGAAACATTGTTGTAGTAATATTCATATTTTCCTTTCCTTTGAAAAGCAAAAAATACACTATCAGAAATAGTGCTTGTATTTTTCTTGCTTTTTATCAAACTAAATATTTATTTTGTATTTTTTATTTATCTGTTTTATAGATAAATAAGTAAAAAATTATTCGTTTTTGCTTTTCGGTTTTTCTTCAAGGTTTTTAAATAATGTTTTATATTCATCATTGTTTAATTTACCGAAAATTTCGTATTCTTTATTAATTTTTTCAAAGTGTTTTTTAAGGGTTAATTTACATTCCTCTTCTGATAAGTTTTTGAAGTTAATTGGTTTATCTTTTGCATTGAATGTAACATTAGTTTGTGCGCATAATTTAGCTTTGTCGTATAAGAAGTTGATATCTCTTCAGAATTCATCACTAATTTCTAGCTTGATTAAGGCAGCAAGCGGTAATAAGAATTCTTTGAGATAGTAGCAGATATCGGTAACAAATCTGGATTGTGAGTGGTCCCAATCAGGATTATCAGCATTAAATAGATAATAGTTGGCTGTTGGATATTTTTCATCATATTGCATTTCCTTGTCAGTATCGTCATCTGTCCTTCTATATTGAAAGGCGACATATTCATGCTTTGTGAGGATTTTATATGGCTCATTTCAACCAGCTGGCATCATTTTTTGCAATGATGATGTTGCCTTATATATTGCTTTACCTAGCAGATTTTTAATTTCCTTCGTTTTTAATTGTTCTTCAATCGGTTTAGTTTTATCAAGTTTAAGTGTATAAATATTATTGAAAAAAGCAATTAAACTTTTATATAAATCTAACTGATAAATCACTGTTTTTGTTGGATCGCATATTATTTCAAATGCTCCTAAAGCAGATGCAATTTTTGTTGCTTGATGAAGTTCTTTACAAGCATCATCAATTACCATTTTTTGTCGATTATTTAATGATTTAATATGTTTAGTTCATTCAATACTTCAGCCATCAACATTGTTAAGTCTATCATAAAATCTTATCGTATTTTTCTTAACAAAATCAACTAACTCATTTTCGGTTGTTCCGGCAGGCCAATCCTTCTCTATAAAGGTAATTGAATCAGACACCCTTTCATTAAAATACCAGTTCAAGGGCATACCAGTTTTATCTGGGCTGTTTTCTCCATAAAAGGCTTTTAACCCTTCTTCAGAACTAATTCCTTCTTCAGAAGCACGATATGCTTGCTCTAATGTTATTTTAGTTTTTTCATTAATTGGACTATCGAAAAATTTTAACTCTTCTATTAATCGATTGTTACGCTCAGAAATATTAAATAATTCATTTTTATTATTATCATTTAAATGTTTGCCAGTTAGTAATGTTTTGAGGTATATTCCATAATAATATAAGCCTGGAATTAATACATCTGTATCACTACCTCAGTCTATTTTAGCAAATCACTCAATACCTAAATAATTACCGATAAAATCGGTTTTATCATGAGATTCAAACTTGATTGCATCAAAATTACGTAATTTAGGTCTTTGTTTACCAGTTCTAGCCTCAGCAATTAGTTTTGTATCAAATTTACTGTCTTCGTAAAGATTGTCCTCATTATTATCTTTTGGCTTTGGTGCTGGCGGTTTAACATTTTCATTCGCCTTGTCATATTTCCTAAAAATATTAAATACTCAACAACTAGATGAAACTAATGGCAAAGCCATTGTTCCACTAGCTAATGTTGTTATTAAACCTAATCTTAATCTGCTTTTTTTCTTCATTTTATAACCTCACCTAATTTTATTTTTTATTATTTAAATTATATATTAATAAAATATTTGCTCAAGCATTATGCAAGCTTATTTTGCATTTTTTGCTTTTGTTAATCTATTTATAGATAATAAAGGTCAAAAATTGCTTTGCCCTCTATATCAATGTCTTGTGTACTTAAAATGTACTTTTTGTGTATTTTCAGACATTTATTCTCCTTTATTTGATTGTTTTTATAATTTGTGATCTTTCTATTGTGACTTTAATATATTCTTTTTTCTTTAAGAGGCCTCTAGCAATTAATATTGCTTGTTCATAATCATTAGAGCTGCCAACAGAAAATGTTTTGGCGTTTTTGTCATAACTCTTGAAGCCACGTAATCTGTAAAATCATTTCGACATAAATCTTCTCCTTATTTGAATGCACACACTGAAGCACGCCCTGCACACGCTAGCACATGGACATTTAGTATACAAGCACGTGTTACACACTCTTGCACGCTCAACAGTCGCATATGAATGCGCACAGTGAAGCACGCCCTGCACACGCTAGCACATGGGCATTTAGTATTCAAGCACGTGTTACACGCTCTAGCACGCTCAACAGTCGCATATATATAATTAATATTTATCATCGTCTGAATACCGAAAACCTCTTTCATTTATTAGTTCTTTAGGAATGTTCTGACTAATTGGCGGTGTAATTGCCTTACTGCTCAACTGATTTGTTCTTTGTTTTCTAACAACACGTGATTCAAGAAATGTAATGTTTTCAACAACTATATCTTGCGTGTATAATGTTTTACCATAGTCATCATATTTAGAAGTTGTAATGTGTCCAACAATTGGAATAATTGAACCTTTAGAATCAAAAGATTTTAAAATTTCTGCTTGATTGTTTCAGGCAATGATTTGTATAAAATTGGTAATCTTATTATTGTTATGTGTTAAGTTATCTACTGCATTGCATTATTTTTTAAATTTGCTAGTATGTTTAACAATTCAAATTCATCAACAATATTATTACTAGTTATAGGTTGCAAACTTTTATCCTTAAATTGAGAAATTACATTTGCTAAAGAATATTTGCCATATCCCTTTGGAGCAACAAAAATTATATTTGGAATTATTTGCTTTTTTGAAGCAATATAATCAATGATTTTATTAAATTTTGTTGCATTTGAAGTTTTGTAAATTTCTTGGTGTTAAATTATATATTTTACTAAAATGACTGTTTTATAGTGTTTTTAGTAAATTAGCTAATTAATTTGCTATATCTAATAAATGGTCATAATATTGCTTTTCTGCCTAGTTTTTTGTTATGCATATCTAATGTATGTTTCTTATGCATTTTAGATGCAATAATTCATAAAGAAACAAATCTATAAAAAACAAAAACAGTAATTACAACATTAATGATAGTATAAGTTCACAATAAAGCTTTAGAAGACATTTTTAATAGTAAAACATCTTCTGAATTAATTGCTAACACTAAAGAAATAGGTAATAATATGATACAAAAAAGAATTAAAGTAAATAGGAATCAAAAATTAACTAATCAAAAAATTCTTTTTTTAGTAAAGTTTGTTATTGGCACTTGTATAGTGCTTTCTAAAGTATAAACTGTGCTTGGAATTTGTTTTGGCATTGTATTATTTTCTTCTGAAAAAACTTCAGAATTTTGATCTTTTTCTTGCATAATATTTATAGATTTTTGCTTATTTTAAAGATAAAATCTTTATCGTTTTTTAAATCTTCACTAACAAATTTCAAGCATTTTTCATTTACACTTACAGCTTTAACCATAAATTTTTTATTAGAATTTAATTCACCACAATATTTTAGTATTCATTCTTTGTTCTTGATTAATCTTAAAATTAAGCTTTGATTCTTTAAAATTTCATCTGGAGCTAATTTAATAACTGCCGGGTGAATATTAAAAGCTTTAAAACAGACATTTAAATCTTTTTTAAGTGAATCAGGTAAATACCTGAAAATATATGGATTTCTTTCTATCAATTTTAAAATCATTTTTTTATTGTTTTTACATTTATCAGAAAAATTATCAAAAATTTTTGTATCTCCTGATTGAATAAAAGAAGCTTTTATAACATCATCATCATTTTGTAATTCTTGAGATGAATAGTAATAAGCTCTTCCATTATTTTTTACAGCTTTTAACACTATTTCTTTATCATTTTTAAATTCTGGAAGACAATACTCAATTCTCATTCCATCATTTGTGACAGCTTTTAGTAAACTTTCTCTGTCTGTATAATATCTAAATTTTTTCATTTTTTTCCTTTCTTCTTTTGTCAGAATTTTCTTTTCAATATCTTAAAAGTTTTAATCTATTGGAGTTTTTATCATTTGAAAATATATATTGTCTAATTTGTTCTAATTTAGAATCATCTTTAAAAAGGAAATTGTAAAATTTGATTTTGTTTATTTTTTTAAAGATTTCAAAAACATTTTTATCTTTATCAAAATCAATTCCTTTAATAAATAAGTTTTTAAATTCTTTTTTGAAATTAGTTGAATATGTCATTAAATCATTTCAAGATTTTAAAGAATTATATTTTTCAAAAAAATCTTTATCATAATAGCAACTTACTCAATTTTTGTCCATTCCATTGTCTGAATCATATTTTCGAATTAAAAAGTTATCTGGATTTTTATTTTCAAAATCATTTATGAAAACGACAGCACCTTTTAATATTATTTTTTCATCAAAACAGTATTTAATAAGTTCTAATTGCTTTGATAAGCTATAAGCTATTTCATATCTTTTCTTAGGATATTTACCATCACTTTTTCTGATTCATAACACAGTTAAGCAAGAAGTATCACCATTTTTTCTAAAAATAAAATCAAAGTTAATTGGCACTTCTATGTTTTTATATTCCTTGCCTTTTAAAATTGTATTTATATCTTTGGCAGTTGTTTTTTCAATAGTATATTTGTTTTCTTTTAACACATTTTTAATTTCTTCTTTTAAAAACTTGAAGTACTCAACATTTTGAAGTCTATAATATTCTAAATTTATAATTTCTTTACTATTATAAGGTTTAGATTTAGATAAAAATTTATTTGGTTTTCTCATAATCAAATCAATTAAACTTTTGACTCTTCTTGATTTAAAAACATCTAATAATTTTTCTTTGAATTTATTATATGTCATTTTTTATTAACTCCTTCTTTTATGCTTCAATAAGCTTGTCTCCTTTTTTGTTTATTATTACTAATTTTGGCGCTTTTGATCTTTCGCCATTTGTTAAACTAGTTATTTTTTCAACATATTCAAGTTGCTTTTGTAAATTCAAAAATGGATAACTTTCATCTGTTGCACATTTAACAAAATCTTTTAAATTTTTTACATGTATCTTAAAAATATGACTTAGGTCAATATAAGATGAAATTTCTTTTACATCATCTAATTCACCTTTTGAATTTACTTGATTATTTCTTATGTCAATTAAGTTTTCTTTATCATTTTTTACCTTAGCATTTTTTGGATAACATGCAACAAAAAACAATCATTCATCATCTTCTGCCATAAATAGAATAGAATTAACTTTTAATTTTAGATAATCTTCAATAATTGCTCGACCTTTAATTTTTAATGGTATGGTTGATAACATATTAATTTTATTATTTGGTGTGTTCATATTTTCTCCTTGTTTTTTATAGTTATTAATTTTGTTTTTCTAATATTAGAATTTTTTCAACTTTAGTTGCTTTGCCCTTACGAGAATTAAGCAATTTAGAAGTTTTAAAATTATTTGTATTGGTATCAATTCCTACTTCTAGGAATGAATAAGGAACTTCATTTTTAATTTCTTTTCAAATGTTTTCTATTAATTCAAAATCATAAACATCACCAATTACTAAACAAACTTTAGCTTTTGGTTTTAATTTAGATGAAATATTTATTAAGTATTTTTTTATAAAATTTGTGTATTCTTTTAAGTCAAGCGAATCTGAAAGTTTGATTTCTTTTTTTAAATTATTTTTTTCAAATCCTAATAATCAAAGTCTTAATCAATTGCTTTTTGTGTAATCGACTAAAGATAAATAGGGTGGTGAAGTAATAACTAAATCAACACTATTATTTTTTAAAAAATTTAAACTATTTAATGAATTTCAATATTTAAAAATGGACTCATAATTTTTAGATAGAAAAATTTCTCATTTAATTTTTAATCTTTTAATTAATTATCGAATATGTCAACATTAGGTTTAGTTAATTTATTTATTTCAGAATATTTTTTAACATAATTTGTAGACAATGAAATAGTATTTGGCATATTTAACGAAAAATAAATTGTTGTACCATCTTTTTTCATTGGACCGTGCATAAGTCCTAATGATAATGCAAGTATCAAATTATCAATATCTTTGTTATTTTTCCAATTAATGCCAATTTCATTTTTTAGAAAAATTAGTTGTTTAAGTGTAAAATCACTATAAAAAACTTTTAAATCATTATATTTTTCACTCGTTATATTTATAGACCTTGGCAAAAGCAAATACTTGTTTTTTAATTCTTTTATTCGTTTTTCAATCTTTTCTATATTTGATATATTTATAAGTTTGGATTTTGACAATACAAATGCATAAGGGTTTAAATCATTACCTACAAATTTTCTATTTAATTTTCTTGATGCATAAGCTGTTGTGCCTCTTCCTGAAAAGTTATCCATAACTGTATCATTTTCCTTGGTATATTTTTTTATAAAATATAACGGCAATTCAACAGGAAACATAGCTAAATAAGAAGATATTGAATTAAAACTATTAGGACTGTATTCCTTGTTTAAATTTGCTTCACAAATAAACATTTTTTTATCTATATCTTTAAAAAACTTAATTGAGTTTCTCATATTTTTTCCTTGTTTTATTTAATAATTTTTTTAATTGCTTTAAATTCATTTCTTTATTTTTAGTTGCATAATAATCAATAACTTTCAAATACACTTCATTTAATATTCCAGGATTATTATTACAATTATTGAGAATATATTCAACAAGACTATTGCTTAATTTAAAATTAGTTTTTAGCCTATTGTAATTAATAATTTGATTAAGATTATTTAAAAAATACTTTTTTAAATTAATATTTTAAAGCCTTCTCATTTTGAGGAATCAATTTGATTTGAAGAATTTATTGTCCCTATAATCGAAAAATTTTTAATCTTAAGACGCTTGTTTGAATAACATATTTTTTCAACTTTATCTAGATTTATCGAGTTATAAATATCAAATAATAATTTTTTGAGCTCTGGTTTTAATTTATTAATATCTTCAATGAATAAGATTGCATTATTTTTTAAATTTGCTAGTATGTTTAACAGTTCAAATTCATCAACAATATTATTACTAGTTATAGGTTGCAAACTTCTATCCTTAAATTGAGAAATTATATTCGCTAAAGAATATTTGCCATATCCCTTTGGAGCAACAAAAATCATATTTGGAATTTTTTTACTTTCTTGAAGCAATATAATCAATGATTTTATTAAATCTTTTTGTCCTCTAAACTCTTTAAAATTTATTGGTGTTAAATTATACATTTTACTAAAATGACTGTTTTATAGTGTTTTTAGTTTATCGGGCATTAATTTAATTGCTTCTTCAATCATTGATTCAACATCTTGATTTAATTCAACTTTAGTTAATGCTGAATCAATTTGAGCTGCTTTAAAGCCTAACATTTTTAATATTTCTTTAGCTTCACTTAATAAATTTGAATTTTTAACTTGTTTTTGTGCATCTTGAGTATTAGTTATTTTAGCTCATTTGTCTTGTAATTCTTCAACAATTATTCTTGCAAGTCTTGGATTAATATATTCTATTTTTGCAATCTCTTCAATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAACCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAATTAAGTCCATAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAATACATTTTTATTTCTTGATTTAATTTAATCCTTTTGTTATCTGGAAACATAACATAGTATCCAACATTATCTGATTCAAAAATTAGATTTTTTCCATCTTTGTAAACTATTTTTCCTTTTCTATATAAAACCATATTTTTTCCTTTCTAATCAAAACTATCTAAGTAGTTTTCATATTCAGACAATATATTTATAAAAATTCTTTGTATTTTGAATGAAATATTTTCAGGAGATTTTTTGAAGCCATAACAATATTTTTCATAAATGTAATCCTGATATTCTTTTTCTTCAATCTGATTATTTCCAAGTAATTTAAAAATTTTTTTAATTTTTTCCATTAATTCATTTTTAATTCTTTGAAATTTGAAATTCTTTTTAAAATCATTACTTTTATTCAATTTAAATGCTTTTAGTAATTCATAATATATATTTAGTTCAAGTAAATTATTTTCTAATCTAAGAGTTTTTAATAATTTATAATCAAATAAAACTTGATTGATTTTTAATAAATTAATATCAGATATACCAAATCCTAAATATTTGTTAATATTTTGCAACTTTGCAATGTCAAAATTGATTATTGTTTTTGAATCTATTAATTTGCTAATTGATTCCATATTTATCTAACAAGCTCTTTTTTAAATAATTCTTGTCAGTTTTTATAATTTGTTTTTCTTTTAATAGATTCATTAATTAATTTTTCAACTTCAAAATTTTTTATATCTAGTAAATAAGCTTCTCTTCTACTGAAGATGCCAACTTTTAGTAATCAATCTATTACTAATTCCATTTTTAAAGTTCTAGATTTCATATTAATAATTTCTTTGTATTCTTTTCTAGTCATATTTTCTCCTTTGTACTTATGCAGTAAATAAATAATTTAATATTTTCACTTGATTTATTAATTAATTTTTGAACTTCAAAATTTATTGGTGTTAAATAATACATTTTACTAAAATGACTGTTTTATAGTGGTTTTGGTTATTAGACGCTAATCTAATTGCTTCTTCTGATAACAATGGCGAAGACTAAAAAGCATATTAAAATAATTAATAATATAAGCAAAATGCTTAATAACAGTCATAAGAAAAATCTTTTATTCATTTGTCTTTCACCTTTTTTCTAATTCATCACAGATTGTGCTATACATCATAGTTAAATATCTATCGAGTGCTTTTGCTTCAATTTCAACAGCTGTGTCTACAAAATGTCATTCATCATATTTATAAGCCTTAACATATAACTCAATTGCTTTAAATCTCATCATAATTCTTTTCACAAATAAATATAATGAGACATCTGACTCTAATAATGACATTGTTTTGTTAACTCGTCTTATATAAATTTTTAATTTGTTTTTTATTTCTTCAGAAGAAAATTCTGAAAATTCTTGGTGAGTCAAATAATGTTTACAAATTTGAGAATATTTATTACATTCGACTACTTTGTTAATATCATCACTTAATGGTGAATCTAATAAATCAAATATTAAATTTTTTATATTTTTTAAATCTTGCTTGTTACTGTTTTCTATTGTTGTTTCTGAAAGTCACATCTTTTGTTCTCCTTTGTACTTATGCATTGAATAAACAATTTAATAATTTTTCACTTGATTCTTGATTAATTTTGCTAGGCAATGTTTGAAATGGAAATTGTGTGATATACAACTCTTTTTTATAATTAATTTTTTTATCAAGATGATTAAATCCACAATCTAGTATGTAAATGATTTTAGATTTGCCTTTACTATTTAATGATGTGAATAAAATTTTTTTATTTTCAAAATCTCATTCTATAAAAACATCATTTTTGTTTTGAGTTTCTAGTAACTTTAAAATCTTAATATTACCTTTATAAAAAAGAACATATGGTGAATAGCGATAATTTTTTAATATTTCTGGAAAATTATCATCAAAAGCTGTTATATACTTAATGCCTTTTTGTTTTAGTTCATTTAATACTTCATCTATTTTAGCTTGAACAATTGTTTTGTAAGTTTTTAAAAATTCATAAATTTTTAGAGTATTTCCTTCATATTTTCAACTTAAATAAACTAATAATTCTTTCATAATTAATAAGATTTGTTCCTTTTTGTAATTGCTTTAAATAAATTAGAATTTTTATCATTTAAATTGAAAATATTTTCTTTAATATTTCTATAAATTTCTTTATTTGAATTTAATTTGGTTCATTCTGACTTTTCCAAATAAAGCAGTGCTCTAAGTGCTTCTTCTGATTGATCTAAATCTGGCATTTTTGAAAAATAATTTTGATTGTGTTCTTTAAATTTTTTTATAAAACTTTCTATCTTTAATCAATCTTCTCTTTTATCTAATATTTCAAAGAAATCAGCTCCATAATAAGGTCTTAAGTCTACTTGTTCGTAATAAAAATTATTAAATAATCTAATATTCTTTATATAATCATTATCTAAAAATCATAAAGCACCCTTAATATTCATTTTTGGGTAAATAATTTTTAAGGCTTCTAATTGTTTAATGATATTCTCACAAGTTTTTTTCATTGCTTTATGTGAATAGCAATCTCTCTTTCTTAAATAAACAACAAACATATCTGTTGGTTCATCTTTAGTTGAATTATTATTCAATCTAAAAATGAAAGATGCATTGATTATTACTTGTTCAGGAATGTCTCCTAAGTGTTGTCCCTCTTTAAGAGGCAAATTGTATCTAAAATTGCTTCTTTGTAAACTAAAACCATATTGAGTAATTAAACATTTAGCGCAATATTGAATAAATTTTTCATAAATTCTTTCTTGATGCTTCAAAAATGATTGTTTAATTTTAGTCTTAACATCAAATGGGTTGCTTAGTGAAACAAAATTAGAAGGCTTATTAATTATTTCTTTTATAAGTTTTGATGTGGCATCATTTGTTAAGGCTCTTTTAAGTTGTGTTTCAAATTGACTATATTCCATAATTTTCTCCTATTGTTTTTAATTCTTCATAAATCTGATAACTAGCATAAACTATTGGCGTTGCTGTATAGGTTCCCATCATAGTACCGGTTATTGCTACTAAAACTAAACAACAAGCACCATTTTGATTATCAAGTGGTTTTTTAGGTTATTTTTTAGAGAGTTTTTGAAGGTGGTTCTCTGACAATTTAACTTAAAAATTCCGCTTTAGTTTGGGATTTGGCTCCATGCTTGCTTGCTCGGTCCATTTTTGATTGCCTCGCCGAGCTGCTTGGCTAATGAGAGAGCAGAGAGAGATTGATTTATACTTGTTCAAGAGATCTCCTGTTGTTTTTTATTTCTTCATAAATCTGATAACAAGCATAAACTCTGTAAGAATTCGAAAAATTCCATAAATCTTGCTTGAATTCTGAATCATTTATATGTTTTAAATCGATAAAAAAATATTTTTCTGATGATTTAGCAAACACAAAATCATAAATATTCTTAAAATTCTTAATTTTTGATCAAGATTTGTTAGATTTTTTGCAAATTTTTAAATAAATTCTCGTAATTTGTGATAATTTTTGGTTTTTCATAGTTAAATAAATTAGTTTATGAATCATTGATTCATATATTAAGACTTTTCCATTACATTCCAAAGTCTTAATAACTTATTCCTTTAATTTTGAATAGACCAATATCAAAAGTTAAATAATGTCAAATGTTTTTCTCAAAATGCAACATTAAAAAACAGATATTTAAAATATCAGTGAATTTTTGTATAACCTACTTTTTATATTTTTCAGTCATTTAGATAAACAACTGCCTTACATTCCAAAGTCATTTTTGTAATAAGTTATATTCTCATAACTAGCCAACGAAAGCATTTAAAAGAATGTGAATTAAGGTCATATTCTCTAACATATTCGAAAGCTTGTAAATCTGAGCTAATTACATCAATTCCAAGAACAGCAACAACTGATTTAAAAAAGACATAACCTTGATTTTTTGATAAATTTAAAAACTTATTGTTAATTAAAACTTTATTATTAGTATTTACTTCATATTTGTTTTTAGACACTTTTTTAGACTTATATGTTAAAAACAAAAATTTAGGTATAAATTTCAATGATGAGATTAGAGATAATCTTCAAACCTCTTCATTTTTTAAGTAATAATATTGTTCTTTTTTAGTTTTTCAATTTTTATAAGTTAATTTTACAAAAGTAAAATTTGTATATCAAGAATCGTTTTTGTTGATGGTTTTAATCAATTTACTTTTAAGCAATTTTTTGTATGCAAAAAATAATGAACTTCTAGCAAAACCATATTTCATTAATCCTGTTAATTCTACAAATTTTCTTCTAGCTCCTGAATATATCAAAAAAATATAAACATAAAAACATACCTTTGAATTCGTTGATACTAATTGATCAAAAATTCTTTTTGATATTGTTATTTTCATTTTAAATAGAGTAGATAAAAAAATCAATGCCTTATCAGCAAAATTTAAATACTTTTTACTGTTTCACATTTTTTCAAATAAAGATTTTGAACTAATCTTTTTGAACAAAGATTCAGTTTTAGTTTCAGGAATTTTATAATTTGCCGTTTTCTCTGGTAATAATTTTTTATTAATTATTATTTGTTTTTTTTCTGATTCTAATTTGTCATAATTTAATCCATAGTTTTGAAAAAATGTTGAAAAAGTATCAAAATTTCCTGTATAAGACATAATTTCACCTCCTTTCTATATTTAGTTTGATAGAAAGAAGAAAAAAACAAGCTTTATGCTTGTTCTCTCATTTAAATTCATTCCCACAATTAGTTTACAGCACCAATAATTTTTACTTAAATATCTATAAAACAGGCTTATTGGTCTGTTTTTAAATTTAATTTTTATTAAAAAATGTTAAATAATTTAATAATAAAGTATAATTTAAATATAATTTTTTTGAGAAGGGAGCTTGTAATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGAGTTTTAGCAACAATATTCCTTATTTGTACAATTGTCTATGCAGTTAGATCTAAATAATAACTAAGCTTGCAAGTGCAAGCTTTTTTATATTCTTTTTCTTAGAAAAATAAAGCTTTCTAGTATAATTTTGATACATATTTTAAAACTAAATGAAAAAGGAGAATTTATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGAGAAATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAATAATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAATAATAAGAAGTTCTCAGTAAGGGAGCTTTTTTATTTTGCTTTGATGATGCAAAATAAATGTTAAATAAAAAATAAGAGCAATTAAGCCCTTATCAACTTTTTAATTAATACAATTTCACACTAACATCTTGTCTATCAGCTTCTTTAGCTTGGAATAATAGAATTGTTGTTAATCTCATACTTGAAGCAACAACACTTTTTGGATATGTAAAGATTTCTTGGTTAAATGTTGTGGCTGAAGTATTGTAAATTCTTCTAGCTGAAGCAATGTTGTTTTCACATTCAACGATTTCATCATTTAAACCTTTAACTAATTCAGCACTCTTAAGTTCTGGATATTTTTCGAATTGAGCCATAAATCTTGTTAAGAATGATTCTGTTTGACTTTCAAAGTTTTGACGTACTGCCATAGCATCTGCTGAAGTATTTCCTGATTGTTCTCGAATTGTAGCAACTGCTGAACGGTATGCTGCAACTTGTGTCATAACATCTTTTTCGAATTTCATGTGTGAACTTACTGATTCAACTAACTTAAGAATTGTTCCTGCTCTTTTAGCCAATTGAGTATCAATTAATGAAGCATCGCTACTTATTTTCATTTGTTGTCTTCTAAAATAATTTACTCAAGAAATATGAATAAAAATTGGAATTAAGAAAATAAATGATATGTATCAAATAAATTTTCCAAAAAAGTTTACTTTAACTGGTACTTCTGTACTCATTGAACGTGGTTCATAGCCTTTTTCATTAATAGCACGACTGTTTTCACGATTGTATAAATTAGCCATTTTTTCTCCTTCTATATAATTATTTTTGTGTTCAATACATAAAAATTATATAAGTTTTTATAGTTATTAAAAACAAAAAGTTAAGCTATGCCAGCTTAACTCGTTTTTTAGATTCATATTGATAATCAGTGGCAAATAAATAACCATCTTTAAATTTTAATTTAGGTACATTTTTAATTTCTTTTTTGGTTGCAAAAAAGATCTTAATTCTTTTATTATCAATAAAAGTGTAAGCACCAGGATTCATCTCATAAGCTCTAATAATATTGATTGCTTCTTGCATGGTTAAGGACTCATTTAAAAGAGCTTTTTCTTTTTCTAATTTGGGACTTAAAGTTACTTTACTTTCATCTTGTTTAATTCTTTTAAGTTTGTTTTGATCTAAATCATTTAATCATTGCACAATGTTATCTGCACTAAGCTTTGAAAGCTTATTAAATAAAGTACTTGCTGTGTCATATTCATCTATTTTAATGGCTTTTTGGACAAAAACATCACCTGCATCCATTTGTTTAATCATTTCCATTAGACTAATACCAGTTTCTTGATCATTATTTAAAAGTGAATATTGAATTGGTGCTGCTCCACGATATTTTGGAAGTAAAGAACCATGAATATTTAGACTTAATTTTTTAGCAATATTTAAAACACTTTCAGGAATATATTGACCAAAGGCACAGGTAATTAAATAGTCATAATTTAAAGTTTGTAATTCATCTTTTATTTGTGATATTTTTTCTGGTTGAAAACATTTAATGTTATATTTTTGAGCTAATAATTTTGTTGGCGTTGGAGCTAATTTATGACCTCTGACACTTGGGCGATCAGGTTGCGAAACAATTGCAACAACTTCAAAGTTGTTGATTAATTTTTCAAATATTTCAACTGCAAAAGCAGGAGTTCCGGCTAATAATAATTTCATATTTTAATTATATAAGGAAAAATAAAAAGTGGAATTAATCCACTTATTTTAATTATTCTTTTGTTCTATGTTCAATGGTTGCTGGTTGATTTGCACTTTTTAAGAATAAATGTAAGAATGTTGAAACTAATGTTACTGCAAATGTTACAAATAAAAAGAATGCAAATGCAACGTGAACGTTAGCATATGATTTATCATCTAATGATCTTGGTCCTGTTACACTTCAAATATTCGCAATAATTCAGCCAACATAACAAATAATTGTTAAAACAACTGCCGCAAATACCAAAATATTTAAAATAATGCTAATTACATTTTTCGAAGCTATTGATTTTACAAATGCTTTAATTGCATAAACAAGAATAGCAATCATTGTAATATGAATGATTATATGTGCTAATAATGAAACAACATAAATAATTTTTACATTATTGCTATCATGTTCAAATATACCTTTAATCACATCAAAATTTTTAAATCATAAGAAGAATCCATAATTTAATCCAGAATGATATATTGCTTCGTATTCATTAAAACTAGATTTTGTGTTTTTTACGATAATTTGATGCAAGTCAACAGCTGATTTTACTATTAAACCAAAGATAACTAAACATGAAATTGCTAAACCAAATAGAATTCATGATGTTTTAGTTAATTTTGTTCTTTTTGTGTTTTCCATTTAATGCCTCCATATAAAAATTGTATAAGCAAAAATTTGAAAATAAAAACAAAAAAATAAAAATAATTTTGACTAAAATGTATATAAAACAAGTAAATAATAGAATTATTTAATAAAAAATAAGGCTTTTTAAAACCTTATAATTTTTTTAAGCTACAACCTTTTTACGACGTTTAACAATAAATCTTCAAAGAGCAACTAGAGCTAAAGCAAGAGGTAAAAGAATTGTTAAAGCAACTACTACTAGATAAATTCTAAGTCACATTTTGTTACGATCTTTTTTGCTTTGCTCAACTTGTTCATGAATTTCAACAGAACGTTCACCGAGAAAATCACTAATGTCAGCTAAACGTTTTTTATCAAATAATCTTATTAAAATATACGCCGCTATTGAAGAAGCCATAATAATAACACTAATAATACAGAATAGAATTGTGTTGCCAAAACCTCTATCAAGTCATTTAGCTCATTCAAAATTGATTTTTAATGTATCAGTTTCATAATATCAACGATTTGACATATAAAGTCCATAAACTATTGCTGTAATAATGCCTAGATATGTTAAGACAAAAACAAGCATTCAAGTTAAATTAACATCTTTAAGTACTATCTTACGATAAGCAATATGAAAAGTACTACTTGAAGTGTAATCACCATTACTTACTGCATCACGATATCTTTGAACTGTGTGAGATCATTGAATATTTTCAATCAGATTTTTAAAACCAATTGCCATTGAGATAAAGGCACTTGATCCAAAAAGTACAAGGTAGCCAACTAATCTTTGTTCTTTGTTGAAGATACCAACTTTAGCATAGACTAATGTGAAGAGAATAATTGAAGCAGCCATAATTACAAATGAAATAGAAAGGATTAAAATCTTAAGCATTTGTTCAGCACGGATGACGCGATAAACACGAGGTGATATTTGATTTTTTGGATCACGATAAACACGACTACTTACACTATTGAATTTGTAGTCGTGGTTATTTTGAACAATTTCGATACTTGCTGAATTTTGAATAGGTTGATATTTTCTCATTCTTGGCCTTTCAATTTTAGTACTCTAATTATAAATGATTATTCGCTATATTTATTAATATAAATTTTAATATTTTATAATTATTTTCAATAATTAAATTTGTGAGATTTTTCTCAACAATTTTTCATTTAAAATTGATGGATTTTACTAATAAAGCTTTTTTATAGATTGATTGGGCAAATATATAAAAATTGTGCCTATGATTAAGCACAATAATTATTTGATTATTATTTGTTTTTGTTTGAAGGTTCTAATTCTTCTTTTTGTTCGTCTGCAACAAGTTTTTTAGCATCTGCTAATTGAATGCTGAAGAAGCTTGTAACACCACGGTTTTGCATTGTTGCACCAAATAATGTGTCAACTCTTGACATTGTTCCATGACGGTGTGTAATAACCAAGAATTGAGTTTTTTCTTTAAGTTGTTGAAGGTATTCTGCATAACGAACAACATTAGCTTCGTCAAGTGCAGCTTCAACTTCGTCAAGGATACAAAGAGGTAATGGACGAGCTTTAAGAATACCAAATAATAATGAGATAGCGATAAGTGATTTTTCACCGCCTGAGAATAATTTAAGGTTTTTAATATTTTTTCCAGGAGGTTGAGCTTCGATTTCAACACCTGATTCTAAAATATCTTTAGGGTTGCTGAAAAATAATTTTGCAGTACCACCACCGAACATTGTTCTAAAGACATTGTTAAATTCATCATTAACATCATTAATAATGTTAGTTAGACGAGTGATAATGATTTTATCCATGTCTGCAATAGCGCTTTCAAGTGTTTCTTTTGCAGCACTTAATTCTCTTTCACCTTCGGCATATTCATTGTAACGTTTTTCTTCTTCAACTAATTGTTCAAGAGCTTCTAAGTTAACGTTACCTAAGCCTTCAATTTCTTCTCTTAATTGACGAACAATTTCTTCAGCTTGATCACGATTCATTTCTAAGTGATGAACTTTTTCAGCTGCTTCAAATGTCATTTTGTATTGAGAAGCAAGTCTTTCTCTACTTTGTTCAAGTAAAGTTTTTGATCTTTCTCTTTCACCAATTTTAGCTGAGAAAGCATCTGTTAATTTACGACGTGTAGCTTCATAATCATTTTTAAGAGCTAAAGTTGAATTGATTTCGGCAGCAATTGTATTTACACGTTCATTTTTAGCTCTGAATTGTGATTCAAGAGCAATTTTTTGACTTTCTAAAACTTCAATTGAAGCTTCAGGGCTTGATGCTGAAACGCCTTCTTTAACTTTGAATTCTTTGTTTGTAATATTTTGAAGTTTTAATTGTAATTCGTCGTAAAGACCTTGTTCACTGCTCTTTTGGGTTTTTAGACTAGTAATTTGTTGTTTTAATTGGCTGTCTAATTCAAGAGCACTACGACGATAGTTTCTTATTTCATTGATTTTTGATTCATCAGCTTGAACTGCTGCTCTCATACCTGGAACAATATTTTCAAGCTCTTTAATTTTTTCATCAAATCCAAGTAATGATTGAGCTGTATTTTTAGCTCCACCAATAATCACACCGCCTGAACGAATGGTGTCACCATCAAGTGAAACTACCATGTAACGACGTTCGAGAATACTTGAAATTTTGTTAGCATTTTCAATGTCGCTAGCAACAATAACATTACCTAAAAGGAATTTATTAAGAATTTCATATTGAGGATCAATTGTTACTAAATTACTTGCAACATCAATAAATCCAGGGTGTCCTTGGATTGCTAAAAGATGATCATCACGAATCATTTTTGGTTGAATTGATGTGAGTGGAATAAATGTAGCACGACCACCATTATTATCTTTTAAGAAGTTAACAGCTTTAACTGCTGTTTCAGCTTTATCAACCACTAAGTGTTGTGAAGCATTAGCTAAAATTGAGTCAATAGCAGGAATATATTCTGTTGTAACTTTAATTAAATCAGCTACAGTTCCTTTTAGTGCTTTTCCAAAGTAAGCTTTATTTTCTAAAATGTTTTTGGTCCCTTTAAATAATAAAGAGTTACTTTCTTTTTGTTGGTTAAGCAAGTTTAACTGTGTTTCAATTTTTAATAAGTCTGTACGTTTTTTGTTAATTTTAATGTTCATTAAATTAATTTCTTCGTCATATTTTGAAATTGTTTTTTGGTTTTCAGCAATTTGGTTTGCTTGATTTTCAATTTCACGAGCGTAGTAATTTAATTTATTTGCATGTCTTGTAAGCATAGTTTTGTATGCTTCGATTTGTTCAGCTTGGTTTGCTTGAATTTCACCAGCAGCAATTAATCTTTCACGTTCATTTTCTTTAGCTAAAGAAATGTTTAATTTGTTTAATCTTTCTTTAACTGTTTCATATTCAATTGAAAGTTCTTTTAATTCGGCTTCAACTTCTGATCTGAATTTAGTATTTTGAGTAATAGCAGCTTCAGCTGATGAAATACGAGCATCAAGATCATTTTTAGTTTCTTGAACACCTTCAAGTTCAGTTACAAGTCTTTCGTACATATCACCAAAAGTTTTGATGTTGTCTACTAAAAGACCAACTTCAACAGCTTTTAATTCTTCACTTTTAGCAATGTAAACTTTAGCTTTTTCTGCTTGTTTTCTTAATGGAATTAATTTTCTTTCAATTTCACTAATAACAAGTTTAATTTGGTCTAAACCTTCTTGAGTTTTAGCCAATTTGCTTAAAGCTTCTTTTTTACGAAATTTGTATTTTGAAACACCAGCAGCTTCTTCAAAAATAGACTTTCTTTCTTCAGGTGTAGCTTCAGCAATATCACTAACTGTACCTTGAGAAATAATAGCTAATGAACTTTTACCAATACCGCTTTCCATAGCAATATCTTTAATATCTTTTTGACGACATGGTTCGCCATTTAAGAAATATTGGTTGTTTCCATTTCCTCTTTCAAGCACACGACTAATTGAAATAGTTTCATGCGGGATTGAACTAATACCATTTCGATTATCAAAAGTTAAAGTAACTACTGCTTTATCCATAGGTTTTGCAGTTTTAGAACCAGCGAAGATAACATCATCCATGTTATCACCACGAAGTTCTTTAGCACTACGTTCACCTAAAACTCATTTGATAGCATCGTTAATATTTGATTTACCAGAACCATTAGGGCCAACAATTCCGGCTACTCCACCATCAAATTTAAGCACAATAGGATCAGCAAATGATTTGAATCCGTGGGCTTCAACTTTAATGAGTTTCATATTACCTCCTTTATTTTTTGAACCACTCAATTCTTGATTTTTTGACGGTTCATAATTATAATTTTAATATTAATATCTAATTTAATTGTAAAAATTTTTATTTTTTCATTTTATGCAATGCATCTAGTGCAGCATTTTCTTCGGCTTCAGCTTTGCTTTTACCAAAACCAATACCATAAATTTTATTGTCGTGAACTGCGTGAGCTTCAAATTCAGTTGGACTTTTTTCACTAACTATATACATAACAGCTAGTTTACTGAATGATTGAATTTGTTCTTGAAAAGCAGTTTTAGCATCTTTAAGATGCTTGATGTCAAAGTCTCCAATTTTGATATCAAAAACAGTTTTGTTTAAAAATTCTTTGACTACTTTTAGATTTGTATCCAAATAAATAGCAGCAATAAAGGCTTCAAAAATGTCAGCGCCAACTTTAACTGAGCGTTTAGCATCTTTACTCATTTGACCAGGTGCAGTTAGTAAAAAATCTTTTAATCCAATTTTAGTGCTAATTTCATTTAGTGACTTTGTGCAAACTAATTTAGAACGAATTAAAGTCATATTACCAGCACTTAAATTTGGAAATTTTTTGAATACAAAATCTGACGAAAGATACTGTAAAATAGCGTCTCCGAGAAATTCAAGATCTTGATAATTGCCAACTCTTTCACTCACATTATTTTTGTTTTGATTTTTTTTGAGTCCAAAATATGAACCGTGAGTTACAGCTTGACGATAGTATCCTAAATTGTGATAATTGATGTTTCAAGCTTTTAAAAATTCTTGAATAGTTTGTTTAATTTCATCCATTATTTATTATTTTTCTTTCTACTCTTCATTAATATTTAAGTCTTTTTTAACCTCAGATAAAATGTGGTTATCTAAAGCTAATTTTATTTGATTAAGTGCTCCATAGTAAGCTTTTTCATCACTACTTCCATGGCTTTTAATTACAACACCATTAACCCCAGCGATTCAAGCAGCTCCAACATTACGATAATCAAGAGTTTCAGCTACATCTTTAAATGCACCACGAGCTAAAAGGTAACCTAATTTTCTAATTGGTTTCAATTTAATTTTGCTAGTTAATAAATTTTTAAAACTTAAAATTGCGCCTTCAAGACTCTTAAGAATTAAGTTACCACCATAGCCATCGATTACTGCTACATCACAAACACCATTTAATAAATCACGAGGTTCTTGAAAGCCAATGTAATTTAATTGATGTTGTTCTTTAAGTTTATTATTTGCTTCTTTAATTAATTCTGTTCCTTTATAGTCTTCAGTTCCGATGTTAATTAATGCACAACGAGGTGCTAAGTTTTTTAATAAAACACGAGCAAAAGAATTTGCAATTTTAGTTCATTCAACTAAATAATCACTTGTGGTTTCTAAGTTAGCTCCAACATCAAGAAGTAAGAATTTACGTCCTGTGATTGTAGGCATTAAGGGCATAAATGCTGCTCTTGAAATGCCTTCTAATCTTTTAACTTTAAAAGTAGCAGCTGCAAGATATGTGCCAGAGTCTCCTGAAGAAAGTACAGCATCTGCTTTTTTATTTGCTACTAAATCAAGTGCAACATTCATTGAGGTATTTTCTCTTAAAGATTGGCGAATGTTTTTAACATCGCTTGGCACTGATGAGTTGTCAATTATTTCTAAATTTTCTGGCATGTTTTTGCCATAATATTTTTCAATTTCAGCCTTAGGACCAACTAAAATAATTGCATAATTTTTGTTGGTTTTTAAAAATAATTTAGTTGCTGCTAAAGCTGAATTAATGCCGTTGTCATTGCCATTAATATCAAATGCTATTTTGTACATTTTGCTCCTTATTTTATTCAAAACCAATAATGAAGTGGTAGTTAGGTTGAGCACCATTCACTACTTCAACTTCAACATCATAATGGCTTTCTAAAAAGTTTTTAATTTCGTTAGCATCAACTTCACTTGCATCATTTCCATAGTAAACTGTAACAAGTTCATGATCACTGTTAATTGATTTTTTAATCAATTTTTTAGCTGCATCAATGTAGTTATTTTCACTAACTAAAATCTTGCCATCAGTAATGCCAATGAATTGACCTTCTTTGATTTTAACATTGTCAATTTTTGTGCTTCTAATTGCTTGGGTTACTTCAGCTGTGGTTACAAGCTTAATAGATTCAGACATTGTTTCACGGTTTTCTTTAGGACTTGATTCAGAATTAAAGTTCATTAAAGCTGTTAATCCTTGAATTTGACTTCTAGTTTGAATAATAAATACCTTTTTATTTGTAATAGCTTGAGCTGCTTGTTCAGCTGCTAGGAAAATGTTTGAGTTATTAGGTAAAATGAAAACGTTTTTGCTATTTACTGAGTTAATTGCATCAATAATATCTTGAGCTGAAGGGTTTTGTGTCTGACCACTTTCAATAATAGCGTCAACTCCAAATTCTTTCATTCTTGCAATAATTCCACTGCCTAAGTTACATGAAATAATGGCACATTTTTTGTTTGATTGTTCATCATTTTGGTTAATAATAACGTTTTTATTACGACTTTCACTTGCTTGACGAGTCATATTTTCTGACTTTATTTTAATAAATTCACCATATTTTTGACCAAAGTTTAACATATCACCAGGTTTTAATGTATGACCATGAACTTTAACAATTTTTTCATCTTGAACTACAACTAAAGAATTAGCTTTTTTAAGTAATGATTTTTCAAAATTAGCTTTATCAAAGTCTTTTTCGTCATTTAATTCAATAATGAATTCAGTACAATAACCAAATTCGCCATCATAAACTTCTTTTGAGCTGATAAAAGTATCAATGTTTTCTTCTGATTCACTAATTTTAATGGCTTCAGCATTTAATGCTGCTTCCATACCACTAATAATTGTATATAATCCTTCACCACCTGAGTCAGTTACGCCAACTTCACGTAAGATCTTTAATTTGTTAGGAGTATTGTCACATGCTTTACGAGCTGCATTTTTAGCTAATTTGAAAAATTCTTCTAGTGAAGTTTTATTATTTACTTCTTTTTCTAATGCTTCTGTTGTTTCTCTAATAACTGTTAAAATGGTACCTTCAACAGGTTTTAAAACTGATGAATAAGCTTTTTTAGTCGCTTGCTTAAATCCTTCTAAAAGTCCTTTAATGTCAACGCTTTCAACTTCTTTAAACGCAATTGCAAAACCTTTAAAAATTTGGCTTAAAATAACACCTGAGTTTCCGCGAGCTCCTAAAAGCATATTTCTTGAAATAGTGTTAGTTACTTCACCTAAGTGATTTGAGTTATTTAAAGCCAAAGCATCAGCAGCAGCTTGAGCTGTTGATGACATGTTAGTTCCTGTGTCGCCATCTGGAACAGGAAAAACATTTAAAGCGTCGATCTTATTTTTAGAGTTAATAATGTTATTAGCTCCGGAAATAAAAAGTTGACAAAAAAGCTTACCGTCTAAAATCTTAGTCATTTGCAAGACCTCCTATAAATACATTTAATTTGCCTAAATGTTCCTTTTTCTTATTGAGTTCGTATCTTAAGTGAGAACTAATTGAGTTAACAACATTTTTTGCATTAGCATATTTTAAAATAATAATTGTAGCGCTGAAATGCCATTCATTGTTTATATTTTGAATACTTAAATATTCTTGGCCTAAATTAGGACATTGTTCTGCATTTTCACAGATTCCATAAACCCCCGGCGTTGATTCAAGAATATTCAAAAAAACTTTTCTAATTTCTTCTAAAGTCATTCTTACTCCTTGTGATAATTAAAAGATTATTTATAATCAAGTAAAACTATTTTAACAAAAAAGAAAAAATACAATAAATAAATATTAAAATTAATATTAAATATAGTAATTAAATTAATTTATTAGGAGAATTATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGAACCAAAGAATTAAAATCAATTTATTACATGTATAATGATTTAAAAATTTTCTTGCAAATTTGTATTTCAAAAGTAAATCAAACTATTTTTTATGAGCTAAATAAATACCTTGAAGAAAATGGAATTTATCTAAATTAAACTTTTAAATTTTAATGAAAACAACTATAAAACAGGACTTTTGGTAAAAATCTTGTTTTATATTTTTTAATTAATTTAATTTTTTGTAATAATAAAAACATTTTTTAATATAATTAAAATAGTTATTTTTTAAAATGTAATTTTCTGCAAAAAATAACTTGAATTTTAGGAGGTAAAATCGCATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGAAAAAATAAAAATATTGAAGGCGATTATGTCAATGCCAGTGCTAATTACTTAAGTAAAAGAGTTAGTGGCCGTGACAAAACTATTATTAATATTGCAAAAATTAAAAAAATGTAAATAAAGTATTAAAATAATAAAGATTAATTTACATTCACAAACATATATTAAATAAATAAAGGAGAAAAATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGAAAAAAATTAGGAATTGGAAATATCACAACTTTAGAAGGTAATTATCGTTACAAAGATCAAAAAGCTTACATGACAAGAATTCAAGAAGTTTCAAATGCAAAAGATACTCTTGCAGGTCACTGAGAAATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGAATAGGTTTAGACAACTTATATCGTTATGGCAAAGCAGCAAGACAAATTTGTTCAAGCAAACCAGAATGAAATGTTGGACGTATTATTGTTAGACCATTTATTGGTGAAAAAGGAAAATACACAAGAACATTCAACCGACATGACTATGCAAACCAACCTCGTCCAATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACGTGACTCAAAATTAGGTAAATTAAATAATGCTATGAAAGAAGACGACTTAATTAATTATAACAAGTGACCACGGAAATGATCCTCTTTATTCAGGATTCAACCACACTCGTGAATTACTTCCTTTAACAATCTTTTCAAAAAGATTCAAAAAACCTAAAGTTCTTGAAGATGTTCTTGGTTTAAGGAACAAGTGGAAATATTGTTGCCCGTAACTGAGGTGTTAAAACTATTGAAGAAACAGGTGACGATATCTTCGATCAATTAGTTTAATATTAAAAATAATACTTGCTTTTGGGCAAGTTTTTATTTTGCTTTTTTGCAATAAATAAAATCAATAATATACTAAAATATTTATATATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTCAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGATTAACAAAAGCGAAAAGTGTAGTTGAAAAATACAAAAAAGGAAATAAATAATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATGAATTAATTCATTTCTTTTTTAAGGATATTGAATTTAAACAAACCGATGATCAATTAGCTTTATATCTTGATGAAAGAATTAATTTTTTAGAAGATCTTTTTTCAAGAACTAGACGATGATATGAAATAATCAAAAACAAAATCAAGAAATTAGAAAAATAAGTTAAGAAATAATACTTATTTCATCTAATATTGCATATAATTAAAAAGTATGAATTTTATTTATGGCTCAGAAAAATTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAATTTTTTGTACAATAGCACCTATTTAATTTATATTCAAATATAAAATAAAGTAAATGAAAACAAAAGAAAAATTATCAAAAAATAATATCTCAAGGAATTACATAAAAAATTAATTGAAAATAATTTTATTTCTAAATTATAGAGAGGAATTTGCATCAATCCACTAGAAGTTATTGACCAATATTATTTTTATGAACTCAAATATAAAGAACATTGTTTTTTTAGAAAATAGTGCAATGTATTTGCAAAACATAAATGATACTTTATATTACAAAATCGAGGCAAATTTTAAGCATGACTATAATACTTCAAAAGTAAATAAAGATATTAAGGCTCATCGCTTAGTTGAATGAAAACTTAATTTAGGAAAAGAAAAAGTTAAAACCATTTTTGGAAATTATGTAACAACATATAATAAAGAAAGAATTATTTGCGATTTAGTTGAAAGTATTGATGATTACGATCTTGAAACAAGACCGTAACTTTTAAAACATCTAAAATTTAAATCAAAATTATAAAAGATTATTTGATTATGGTAAAAAAATGGGTATTATAAAAAAATTATGAAAGTTTGAAAAAAATTAAAAAACAGTTTAAAAGAATATGGCAATCAATAAAGATAAGCGAAAATATTCAATTGTTGAATTTAAAAATGAGGGTTCTAAACTAAGGGACCTCATTTTTGCAACTTGAATTTGAAGTTTAAACAAAATTAACAAAAAAATGATAAAAATTGATATAATAAATCAAGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAATGCTAAAACACTTGCGCGAGCAGGCGTTTTTTATTTTATTTTTATACAACAACTGTGCCTAATTAAATATATAATTGAATAAAAATATTAATATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAATTGTAAAAATTGATAGGGGAATATATGTAGATATTGATACTGCAGATGATGAACATTATGTATTTCAATTGAAATACAAAAAGTGCATTTTTTTTGAATTAGTGCAATGTTTTTGCAAAAAATTACCAATGTTTTATATCATAGAACAGAAGCAAATTTTAGTACAAATTACAATACATCAAAAATTGATAAGAAAATTAAATGCCATAGAATTATAGATTGAAAACTAAATTTAGGAGTTGAAAAAGTTATAATAATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAATATTGTATTTACTTGAATAACTATAAAACAGCTAAATTAGAAAAATTTTAAACTATCCAGTTTGTTAAATTATTATTAAAAAAGTAATTTTAATGTATCATTTAATAAGTATTTTAAAAATATAATTTTTAAATAAACAAAGGAGAATATTATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGAGTGTTTCATGATGGAATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAAGCCTATTTTATAGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCATATCAAATTTTGATCAAGCAAGCTATAAGCTTGTTTTTTATTTAACTACTTGATTTTGAAAATTAAAAAATAATAATTTGAACAATTTAACTTAAATTTTAATATAATTGATTTATGCAAAGTAATAATTGACAAGCAAAAAATGGTGCTAATAATCAACAAGCACAAGATAATTTTAAAAACAAAGAAAAACCAAAGCCAAAAGAAAAAATTAAATTTAGAGGAACTAAATTTAATTTTAAAAAAGTTTTAATAAGTATTATTGTCTTCATTTTATTTTTTTTGATTAGTTTAAGTTCATTGATTACTTTCTATGTATTTAAAAATGAAACGATGCAAATAACATCACTTTTCTTATTATCTCTATTTGGTTTTATATTGTTAGTTTTTTGAATTATTCTTTTAATAGAATTATCAACAAAGAAATGATGATATTATTTCACTCTAACAATGTTTGGACCTTTTACCTTATTTATTCTTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGAAAAAGAGATACAACTACTTTTAATGTAGAAGAAAAAATACAACAAAATAATTCACAAAAAAATTCTGAAGAAGAAGCAATAATTGTTACTAACTTCCTTGAAGATAACGAAGATTAAAAGCTGCACTTAATCAGTTTTTTTATCGCATTAAAAAACCTAGACAAATCAAGTTGTCTAGAGGTTTTTTAAGCATGAACTAAATTGATTTTATTAGCTTGTTTGCTTTAATGAAAATAATTATTTTCTTAATTTCATTTGCAATAGTTAAACCTAAAGCTAAAGGTAATGACATTCAAATTAAGATACCATTGCTTGCATAAATGCATTCATACATTCTAAATACTTTAGCTACTTGAGGCACTAATGCAACAAATAAAGTTGAGAAAGTTGAGAAGCTAGCAGCTAAAACAACTGGTCAGTATTTTTTAATATTGCTAATAAAAATACTTTTATCACTCATTAAATTAATACTATTTAATGAAGCTGCAACACCCATTGTAATAAATGCTGTTGTTGAAGCGATTTTAACTAAATTAGCTGGATCATTTTTAATAGCTCACTCAGCTCCAAGTGCATAAGAAATAAGCGATAATAAACTTACTAAAGCACTTTGTCAAAGTAATTTAATTCCCATTCCTCGAGCAAAAATACTTTCGCTTTTTGAAAATGGTTTACGTTTCATAACATCTTTTCCACTATCAACAAAACCAAGTGCAATAGCTGGAAGTCCATGAGTTAATAAATTAATTCATAATAATTGGCTAGCTCCAAAAATGTAAAATCCATCTGGATAATAATCTTTATATGCTAAGAAGAAAGCGACCATACCAATAAGCATTACTAAAATTTCTGTTAGTGAGCTAATTAATAAGTTAAGTATCACTGTTTTGATTTTGTCAAAAATTAAACGACCATTTCTTACTGAATTAACAATAGTATTGAAGTTATCGTCAACTAAGATTAAATCAGCTGCTTGTTTTGAAACATCAGTTCCTGTAATTCCCATAGCACAACCAATGTCACTAGTTTTAAGAGCGGGAGCATCATTAACACCATCACCAGTCATAGCAACTACTTTTTGGTGTGATTGTCATGCTTGAACAATTCTTAATTTGTCTTCAGGATTAACACGAGCATAAACTGAAATATGTTCAACTTTAGCTCTTAATTCTTCTTCACTCATTTTGGCTAAATCTTTGCCTTCAAGACAAGTATCGCTTTCTTGATAAATGCCTAAATTTTGAGCTATAGCTCTTGCTGTAGTTACTTGATCACCTGTGATCATAACAACTTTAATACCAGCACCTTGAGCTTCTCTAATTGAATTTGCAACCTCTGCCCGAGGTGGATCAATCATAGCAACAAGGCCAACAAATTCTAGATCATTTTCGTCCTTAAAGTCTATTTTACTGTGCTTTACGACTTTTTTAGCAACTGCTAAAACACGATAAGATTTGTCAGCTCATTGGTGATTAATTTCTTTAATATCATCAATAGTTAAATTATTACATCTTTTTAATAAAACATCAGGAGCACCTTTAGTAACTAAAAGCATTCTATCTTTTTCTTTGAATACTACAGAGTGCATTTTACGATTTGAATCGAAAGGCAAAGATGCTAAAATTGGATATTCTTTAAGTAATTCTTCTTTTTTATAACCATAAGAATAAGAGTATCTTAAAAGGCCTGTTTCAGTGGGATCACCTACTTCTTTTCATTCATTATTAGCTTCTAAAGTAATAAAAGCATCGTTACATAAAGCTAAATATTTAGCAAGCGGCCTAAATAATTCTTCTTTCTTGTTTGCACTTCAAAGAGTTCTAAAACGATAAGCATCAACTACTGTCATTTTATTTTCTGTTAGTGTTCCTGTTTTATCACTACAAATAATTGAAGCAGAACCTAGGGTTTCAACTGCTAAAAGGTTTTTAACCAACCCTTTTTGTTTGGACATCTGACTAACACCAATTGATAATAGAACAGTGGTAAAAGTAATAAGGCCTTCTGGAATAGCAGCAACTGCAAGTGAGATTCCAGTGACTAAAGCATTAGTATAAACTTGTGAATTTGTTCAATTTCCTGAGTGAACATTATTTAAAATAATTTGTAAAATAAAGCTTACGAATAAAAGGATAATTCCACCATAACCAAATCATTTGCTTAATTTATTAAGTTTGATTTGTAGTGGTGTTGCATTTTTATTTTGTTTTTGAATCATTGAATTAATTTTGCCAATTTCAGTTTCACTAGCTGTATGTTCAACTACAAAAGTTGCCCGTCCATTAATTACATAAGTGCCTGAGTAAACTAAGTGTTTGTTGTTAGCTAAGATTTTGTCATCAGTTGCATTTAAATTTGCGACTTTACTTACTGGTAAACTTTCACCAGTTAATGAAGCTTCTACAACTTCTAAGTTTGAAGCTTCAATTAAGCGACCATCAGCATTAATTGTATCTCCTGCAGCTAAAACAACTAAGTCACCAGGCACTAATAAATTAGCGGGAATAATTTGGACTTTACCATCCCTAATTACTGTAGCATTGACCTGATTAACTTTTTCAAGGGCTCGCACCGCTTGATCACTTTTGACCTCTTGATAAGCTCCTAACATACTATTTAAAATAATAACTAGCATAATAATTCCAGGTTCAACATAAGAGATAATTAAATCGGTTGTGCTTTTTCCGCCTTGAAAATGTTCAACAATTGCCAAAGTTAAACTAATTGCTGCCGCTATGATTAAAAGAATAACCATTGGATCAACAAATTGTCTAAAGAAAGCAACTATTGGATTTAGTTTTTTCTTTTTAATTAAAGCGTTTTCACCATACTTTGCAAGGTTTGCTTCAACTTCTTTTGAAGTCAAGCCTTGATAATTGGAATAGTCTTTTTTGCTCATCTTTTCTCCTTAAATAAACAATGTTTTTATTATATATTAATTAATATAATATAGTTTAAAAAACTTGTTAGTTGCTATTAAAAAATATAAAAAACACTAATTCTTAAAATTAATGTTCTTCTTATCTTACTTTTTAAGTTTTTATTGTTTTCTCATTTCTTTGTGTATCTAGCTCTTCTATTTGCTTTTAATTCGTTAAATCTTATAATTATATCTCGAGTTCTCTCAACTATTTCATTTCAACGTCTTTCATTTTCTTTAGGATCTTCGTTCAATTTTGGAAAGAAGCAACAATCTTCATCATGCTTTACTTCTTCTTTATTGTTTTCATTAACTTTGCCATTTTTGATTTTTTTTACATTTTACATATTAATACTTTCCTTTTAAATTATTCATAATAATTTTATTTAAATCTTAAAAAAATAAAAAAATGGCGGAACAGAGAGGATTCGAACCTCCGCGGGTGTTGCCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCGACTTGAGTACTGTTCCACAAGTGCTTTTTATTATATAACAAAAGTTTAATAAATGAAAATAAAAAGCAGGCTAATTGCCTACTTGTCTTTAATGAATGTCGTTGGGATCTAAAATTAAATCAAAATATATAGGTGAGTGATCGCTAATTGCATGAAAGATATATTTAAAATCTGCATTGTAAGTTTTTTTTCTAATAGTATCAACATATGTTTTTCATTCTTCTATTGTTTGATAATCTTTGAGAATATTGTCTTTAAAAATATCGTATAAAGGAAAGAAGTTCGCGTTTTCATATTTTAAATTAGTTCTAGTAAAAATCTTGTCATAAGGTTCAGAATATTTTCCTCATGTTTGATTACTCAATGAACTTTTGTGTTGTTCTGAGTCAGTTAACAGTGCTTTATAATATTGGTCTTTTAGTATTTCATTAAAAGCGGTTGATTGATTTCCTAATTTTATGTTGGTATCTCCCATAAAAATAAGATCATCATTGTCACCATCTTGTTGATCAAATCAATCCATAAGGTTCTTTAAGTTGTAAGCTTCATCAGCTTCTTGAGCGCCTTGCTTACTTAATAAACTTGATTTTGATTCGCCACTTTTTGCTGCTCCTGGACTATCTAAATGATCTATAACAAAAGTGAAATTATTTTGAATTGGTCCTAAAGTTTTAAATTTAACTCCCATTGGAGGTCTAACATAACCAAGATTATTGTTTTCTTCATTAACAACAAAAGTGCTATTGTCATAACCTAATCATTCCTTGCCATTAATAAATTTTTCAATAGCAATCTTATCTTTTTTATAAATAAAGGCTGGACATTCATGTTGTTTTGAAGTTGGGGCTAATTTAGGATTGTGAATAGTATTATCAGAAGCTATTAATTCTCAATTGTTGTTTCCCGTAAATTTTTTTAAGTATTCACAAAATTTTTGTGCTGAAGCTTTTGTTTTATTTTCAATAAGCCCAATAACATCAAGATTTAAATGTTCAATAACACTAGCTAAACCATATTTTTTGTATTCTTCAGATGTTTCATATAAATTTAAAATATTCCACATTCCGATACGAATTTTAGATTTAGCTACTTTAAAACCAGTAATTTCAAATACCTGAATATCACTAAAAGTATTGTCTTTATTATTTATTTGAAAATGAACTTTGACTGAAGTTGGTAAGCACTCTAATTTGTTATAAGTAATGTTGTATTTTGTTGAGTCAATGTTTGCTACTTTTAGTTGAGTATAATCAATGACACTATTACTAAAAATGTTTTGTTTATTATTAACATCAAAAACAATTTTTTTAGCTATTTCATTTAATTCTTCAACTGTTTCGCCACTAATATTTTTGCTAACTTTAAAGCCTGTTAATATATATGTAAACTCATTACTTTCTAAGTTATAATTTTTATTTTTTAATGTAAAAGTTACTTTAATTTCATTGTTAGTTTTAGCTCAATTTTTAAAAATAACTTCTTCTGTGTTTTTATCAAAACCACTATAAAACAAGTCTTTTAGTAAAATTTCAGAGGTTAATTTTTCAGCTATTTTTTCAACTTCAACCTTAACATTTTTAACGGATTTATTAAGCTTATCAACAATTTCTTGTTTGTTAATTTTATTAGCTTTAAAACCTGTGAAAGTGATTTTAAAACTTTTACTTTTTAGATTATTTTTTTCATCTTTTAATACAAAATTAACAGTTATTGTACCTTCTTGATCATCTTTTTTTATGCTTTTAACTTCAACTAAATATGTTGCGCAATTGTAAAATTTTAATTCATTTTTAATCTCTTCATCATTTAAATTTTTAACATCAATTTGAGCTTTTTTACTTGCTTCAAGATTATCTTTATCTCATCAAATTTTGATTTTTTCAACCGCATTATTTAAGTCATTTTGAATTTCATCTTTGTTTTTTTCTTGTGTTTTATTCATTTCAAAAAAGCTACATTGAGTTGATAAAAAAGGTAAGGATATTAAAGAAAAAGACCCTACCAATAATTTAATAAATTGTTTTTTCTTCATAATATAAACACCACATTCTTATATATAAAATTATATTAAAAAAATTAAAATTGTTTAAATTTTTATTTAGTTGACTATTTTCATATTATTTCCAAAAGTGGGGGTGGTAAGTTTAAAGAAAAATGCATTTTCTTAAATTGACTGCTTAAATTTGATTTATTCTAAAAGTCATCATTATGAATTTTGTGTTTGAATCATCTTTTGTCATAATGTTATTTGTCTCAATATTTTCTTCTTTTTATTTGTATAAAAAGCTGAAAAAATAGAATTTGTAGAACAACTGTTCCACCAATTAATAAAGATATTTTTTTCATATTTTTTATTTTGACATTTCTTTTTTGCAAATACATGATTATTTGCATAACTAGTATATAAAATATTTTTTGCTTTATTCAAATGCGAAATAAACAAAAAATAAAAAAGATAAACAACTAAAGAAAATTGTTTTTTAAGAATTATTTAATTCTTTTTTTCTTTTATTGTTTAAACTTTTTATAATTTATTAATTTGTGAAAAAATTAAAATTCTTTAACTTTTTCATTAATAAAACTTTTAATCTTGTCTGCAATTTCAGGTTCTTCTAAAGCATAATCAATGTTTGCTTTAACAAAGCCTTCCATTGATCCAAGATCATATCTGATGCCTTCAAATTCATAGGCATAAATTTTTTGTTTATATTTTTTCATTAATTCATCAAATGCATCAACTGCTTGAATTTCATTTTTGCCATCATATTCAATTCTAGATAAGATATCTAAAATTTCTGGATTAAAGACATAACGTCCAAGTATAGCTTTATTTGATGGTGCTTTATCAGCCTTAGGTTTTTCAACTGCACCATTAATTTCAAAATATTTCTTATCTTTATCTTTCAAGTTTATTGGATTAACAATTCCATATTTATGAATATATTCTTTTGCTACTGATTGTACACCAATAACATTTGCTCCAGTTTTATTATAAAACTCGATTAATTGCTTAATAGCAGGAGTTTTTGATTTAATTAAGTCATCACCTAAAATAATAGCAAACGGCTCATTGTCAATATATTCTTTACAACTTGCAAGTGCATGACCTAAACCAAATTGTTCCTTTTGAATTACTCTAGTAATACATGAAGAGCGATTTGTTCTTTTAACTTTTTCTAAAATATCTTTTTTACCTTTGATAATTAATTCTGTTTGTAAATCTTGATCAACTGAGAAAAATTTTAATAAATCCTTTTTCCTTTCACTTAAAACAATTAAAATTTCCTCAATACCTGAATCAAGTGCTTCATTAACTAATCTATCAATAACAGGTGTATCAAGAATAGGCACTAATTCTTTATGAATAATTTTTGTCATAGGTAAAAATCTTGTTCCTCAGCCAGCTGCAGGAATAATCAATTTTTTAATTTTTTTAATTTCATTCTCTTTTGTTGTCATGTTCCTAATTATACTATATTGTTTTTATTTTGGAATGCTTCTCTAGCAATGTAAAAATTTGAAAAAAATAAAAAATCAGTCTAATGACTGACTAAATATTTTGGTGCTTAAACATATGGCGATCTTAAAGGGACTCGAACCCTTAACCTTTGGGGCCGAAACCCAACACTCTGTCCATTGAGCTATAAGATCAAGTTAATTAACTAACTCTATTATAAGTGATTATTTAATATATGTCTCAATTTAATTTTTTCTTTAAAAATTTTACTATCTCGCAGTCTACAGCTTCTATTGCATTAATTGTACTGAGTTGCCTAGTTGCTTCGTTACAACTAATTTTGCAACTAAAAATATAAAATCATCTGAAAAACTTTGTAATCTTTTGGCTTCATTATTAACTAAATAAGTGTCTCAGTTTCTTCTATTATTTAATCATGAACCTTTACCACCTACTCTTAATGTATAACCTATTTCTTTATTTACTTCTGCATTGTCAAAAACTCAATTAAGTTTTTTGGTTAATGGTATTGGTTTTGGAAAAGTGAAATTATATTTAAAATCTTTTCTTATTCCAAGCATAAATAATCTTGGTCTATGCTGTTGCAAATTAAAATCAAAAGCTTTAACTATTTAAAAATGAAAATTGTAATCTAATTCATTCAATGTATTTTGGATAATTTAAAAACTTTTACCATTATTATAATTTTTTAATTCTATAACATTTTCTAGAAAAACAATTTTAGGTTGCTTTACTTTTATTATTTCAGCTATATTAAAAAATAAAGTACATCTTGTATCTCCAAAACCTTTTTTATAGCCTATTTAGGAAAAAGGCTAACATGTAAAACTTGCACAAAGTACATTATGATCAGATATATTTTTGGATTTATTAAAGTTATATCACCTGCAAAAAAATTAGTTGGTTCATTATTTTCATTGAATAAATTTTTGCTATTTTTAAAATTTGTCGCATATGCTTTTCTTGCATTTTTATCTCATTCTGATGCGAAAACGCGTTGACCACCTAAATTGTTCATAGCAATATGAAATCCACCTATTCCATCAAATATGTTAATAAATTTAAAGTTTAATTGTTCCTTTTTATTAATCGTTATTAATAATTTAACATTATTTTTGTTTTATAAATAAAATCAGTTTTATCATAAAAAGGAATAAAAAAACTTCGTCAAGACGAAGTACATTAAATAATGATATGGTGGCTCCGGCAGGAATCGAACCAGCGACACACGGAGCTTCAGTCCGTTGCTCTACCAACTGAGCTACAGAGCCATAATGGCGGTCCAGACGGGAATTGAACCCGCGATCTTCTCCGTGACAGGGAGACGTATTAAACCACTTTACCACTGGACCAATTGGTGGCGGAGGCTGGACTCGAACCAGCGGCCTCGAGGTTATGAGCCTCGCGAGATACCAAACTTCTCCACTCCGCGATGTGATATTTTTTTATTTAGGTGTTCATAAAGAACAAATGGCGGGTGATGAGGGATTCGAACCCCCGCGGGCCGTGAAGCCCCTGCTAGTTTTCAAGACTAGTCCCTTCAGCCAGGCTTGGGTAATCACCCTTGGTGGACCCAACTGGATTCGAACCAGTAACCAACCGGTTATGAGCCGGTTGCTCTGCCGTTGAGCTATAGGTCCAAAGCAGTTAGGATTTTCTCCATATAATGGTAGCGTCGATGAGAGTCGAACTCATGACCTTCCGGGTATGAACCGGATGCTCTAACCAACTGAGCTACAACGCCATAAATGGTGGAGGATATGGGATTCGAACCCACCACCCCTTGCGTGCAAGGCAAGTGCTCTAGCCAAATGAGCTAATCCCCCACATGGTGAAGAAGACAGGATTTGAACCTGCGACCACTACGTCCCAAACGTAGCGCTCTGCCAAGCTGAGCTACTTCTCCTACTTTCAATGCTTCACTTTCTAAATCAATAAGATAATAAAAAGCATATATATTATATACTAATTTGTCTTTTTGTAATAGAATAATTTATTTTTTTTATAATAATTTTTTTTAACTTATAAAGCTTATTTATAGTGTTTTTAGTCAAATTGTTAATTGTTTTCAAAAAAGAATTTTATTTTTATTGCTTAAGAAATTAAAATAGTCATTTAAATAAATTGCTTATTTTTCACAAAAATTCCATTAAGCTTTTGAATATCTAAATGTTTGCTAAAATATATATAATAATTTGATAAGGATGAACAATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTCGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGATATAAATTGAGGATAAAAAAGGATTGATTTGATCTTTCAATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGACGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATAAAGTGGCTTGATTAAAAGAATTTGTAGAGCAATTCAAAGACTAATTAAAATAGTATTAAAACTATTTTAATTTTTTTACTTTTTTAATTGTTTTATAGGCTTTTTGGTAAAAAATGAAATAAAAAATGTCACACTTGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAGCTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAAGTCTCACAGTCAAGCACACTTCTACCTTTGCGCTCTACATACGGTTTCTGACCGTACTGAGTGTACCTTTGAACGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCGTCACGCACTGTCCACCCACCTGATGATGATGGCATGTTAGAAACTCAATATACCAAGGGTGGTATTTCAAGGATGACTCCACGAGAACTAGCGTCCTCGCTTCAAAGTCTCCCACCTATCCTACACATGTTAGACCAAGTTCCAATACGAAACTGTAGTAAAGCTCCATGGGGTCTTTTCGTCTTGATGCGGGTACCCAGCGTTTTCACTGGGACCATAATTTCACCGAGTCTAGTGTTGAGACAGTTGAGAGATCATTGCGCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCATTTCAACGCTTCGCAAATGCTAACGCATCCACTTAACCTTCGGGCACTGGGCAGGCTTCACCCCCTATACATCACCTTGCGGTTTAGCAGAGAGCTGTGTTTTTGATAAACAGTTGCCCCTCATAATTTACTGTGGCCTAAGTGTTACCTTAGGCACCCCTTCTTGCGAACTTACGGGGTCATTTTGCAGAGTTCCTTAACACTAGTTTTCTCGCTCGCCTTAGAATACTCATCTCGGGGACGTGTGTCCGTTCTCGGTACAGGTTTCCATAATATTAAGTTTAGAAGCTTTTCTTGGAAGTGTGGAATTATCTAATTCGGTTTGACCCTATGCATCACGCCTCCCGGTTATAGACTGCGGATTTGCCTACAGTCACCAGTTAACGCTTACCCCACAATCCAGTAAGTGGTAAGATTATCCTCCTCCGTCACTCCATCACTATTATAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCGTCTTAGGTCCTGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCGTAGATTCTCACTACGAATCGTTACTCATACCGGCATTCTCACTTCCTAGCGCTCCACCAGTCCTCACGGTCTGACTTCATTGCCCTAGGAACGCTCCTCTAACGTAAATATAATTACCCGCGGCTTCGGTATCGTGTTTTAGTCCCGTTAAATTGTTGGCGCAAGGTCTCTTGACTAGTGAGCTATTACGCACTCTTTAAAAGGTGGCTGCTTCTGAGCCAACTTCCTAGTTGTTTATGAAACCTCACAACCTTTCTCACTTAACACGATTTTGGGACCTTAGCCGGCGATCTGGGTTGTTGCCCTCGCGAGCCGGGACGTTAGCACCCCGGTTCCGACTGCATAGCAATACATAGCGGTATTCGGAGTTTGATTATAGTCAGTACCCCTAGGCGAGGCCATTCCATATTCAGTGCTCTACCACCACTACTTAACACTACACGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCACTATTCACAAGTCATCCGGGCACTTTTCAGCGTACTACGGTTCGGCCCTCCGCTTGGGGTTAGCCAAGTTTCAGCCTGCTCATGAATAGATCACATGGTTTCGGGTATATGACAACATACTAAGACGCCCTATTAAGACTCGATTTCTCTACGGCTCCGCTTTTTTCTGCTTAACCTCGCATGCTGTCATAACTCGCCGGTCCATACTGCAAGATGTACGCCATCACCCATAAACGGGCTCTGACTAATTGTAAGTAAGTGGTTTCAGAATCTATTTCACTCCCCTCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGGGTTCCACGTGCCCCGCCCTACTCAGGATACGATCAGAAGACTTAACAATTTCACATACGGGGGTATCACCCTCTATGCCGCTTCTTCCCAAAAGCTTCTGCTATCATTAAGTTTTGTAACTTCATGTAGATCGTCCTACAACCCCCATTGCTGGGTTTGGGCTCTTTCTCGTTCGCTCGCCGCTACTAAAGAAATCATTGTTTATTTTCTCTTCCTCTTGCTACTAAGATGTTTCAGTTCACAAGGTGTCTCACTCAAGTTCCTATGAATTCAGAACAAGGCAACTAGGCATTACCCTAGTTAGGTTTCCCCATTCGGAAATCCCCGTTTCATAGCATATTTCCGGCTCCACGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTTCAGACCCAAGGCATCCACCACAAACTCTTCCTTATTTAAAAGTATTTTCCTATTGTTATAGTTTGTTATGATGTGTATTTTAAGACATTTCAATGAATCATAATTAATATGATTTTCTCGATGAAATCAAAACAAATTAACCATTTAATTTAATTTTGATGTCGTTGTAAATATCAAAAAATTTACTATTCAGTTTTCAAAGAACAAATGAGAGAAAAAAATATTGTTCTCTCAAAACTAGATATAAAGCTTTAGACCCATAAAAAAGCCACATAACAAAAAATATATAAATAAAATAGGTCTAGTGAAATAAGGTTAATAGTACTTTAAAAAGTACTCTTTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACCCCAGTCACCAGTCCTGCCTTAGGCAGTTTGTTTATAAACCGACTTCGGGCATTACCAGCTCCCATGGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGTAGCGTAGCTGATCTACGATTACTAGCGATTCCGACTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGACCGGTTTTTTGAGGTTTGCTCCATGTCACCACTTCGCTTCTCTTTGTACCGGCCATTGTAGCACGTGTGTTGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCGATTACTCGGGCAGTCTCCTTAGAGTCCTCAACTAAATGGTAGTAACTAAGGATAAGGGTTGCGCTCGTTGCAGGACTTAACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCATTCTGTTAACCTCCACTATGTCTCCATAGCTTTGCAGAAGATGTCAAGAGTGGGTAAGGTTCTACGCGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGATCCCCGTCAATTCCTTTAAGTTTTATTCTTGCGAACGTACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGCCGATGAGTTCCCCATCAGCTAATGATCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGTATATGTCCAGTTAGCTGCCTTCGCCATCTTGGTGTTCTTCCTTATATCTACGCATTTCACCGCTTCACAAGGAATTCCGCTAACCTCTACACAACTCTAGCCTGCCAGTATCCAACGCGTTTTGGGGTTGAGCCCCAAAATTTAACGCCAGACTTAACAAACAACCTACAGACGCTTTACGCCCAATAATTCCGGATAACGCTTGCAACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGTTGCTTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTGCGCACTCTATGGTTTATAGATTGCTTTTTAATTATATATAATAAAAAATTAAAGAGAAGAAAATTTTTTTATTTTTTTTTAAAAATTTTGGCAAGTTAATTTTAAGAGCAAAAAAACTAATTTTTTGCCTATAAAATAAGATATTTTGGTTACTTTAATAATTTATTTAGTTAGCTAAATATTGCAAAAAATATGAAATAAAAAAGCAAGGTTACTCCTTACTTCTTTATATTATTAATAGAAATACTTTTTTATAGACTAACAATAGTTAGGTTGCAAACTATAATAAAGTAACTTTTCCACCTTTAGCTTCGATTGCATCAATAGCACTTTTTGAAGCTGCATGAAGATTAACATTGAATTTTTTAGTTACTTGACCATTACCCAATAATTTAACAGGCATTTGTCTTTTAATCAAATTAGCTTTGAATAATGCTTCTAAGTCAATAGTTGCATTTGCTTTAAAGTTGTTTTCTAAATCAACTAAATTAATAATTTGGTATTCGATGTGGTTAACATTGGTAAATCCACGTTTTCCAATACGACGGAATCAAGGTGTTTGACCACCTTCAAATCCTAGTCTGTGACCCTTACGTTTGTTTTGTCCACTTTGACCTTTACCAGCTTGTTTACCTTTTCCAGCAGCATGTCCGCGACCAACACGGTGTTTTTCAACACGTGAGCCAGCGGTAGGTCTTAATGATTGTAATGTTGAGTTCATTTTAGTCCTTTCCTTTATGTAAATAAATTTTTAATTTTACTTAGTCTAAATTAATTAGGCTAAAAGATCTTTAACATCTTTACCACGAATGTCAGCAATTTGTTCTGGTGTTCTTAAAGCTTGAAGAGCTTTTAAAGTTGCTCTAACAATGTTTGCTTTTGAACGTGAACCATAAGTTTTGGTATAAATATCGGTGTAACCTGCAAGTTCAACAACTGCTCTAACTGTACCACTAGCAACAATACCTTTACCTTTTGTAGCAGGTTTAAGCATAACTCTTGAAGCTAAGAATTTAGCATGAACTTCATGAGGTACTGTGATTTTGTTTTGAATTGGAACTGTAATAAGGTGATTTTTAGCATCTTTAACTGCTTTTTTGATTGAGTCAGGAACTTCGTTTGCTTTTCCATGCCCAAAACCAACACGTCCTTTTTTGTCACCAACAACTACAAAAGCTGCAAATGAGAAACGACGTCCACCTTTAACAACTTTAGTTACACGTGAGATATTAACAACTTTTTCGCTGAATTCATTGTCGCTAGCATCAAAGTGATTACGACGGTCACGGCGATTGCCTTTTTCGAATTTACCTTGTGGACGAGAGTTTTGAGAAATTTTTTGACCTTGAGGTCTTTTTTCTCAAATTTGTTTTTTATCTTTGTTTTCAGCTTTAGCTTTAGGAGCTACAACTTCTTTAGTTCCTTGAGCTTGGGCTTCTTTAGGTTGCTCTTTATTAACAACTTTCTTTACTTCTGCTTCTGCCATAATTAGAATTTAACTCCTTTAGCTATACTTCTAACACCTTCTGCGAAGGCTTTAACACGTCCATGATAAATGTAACCTGAACGATCGAATACTATTTCTTTTAATTTAAGGGCTGCAATTTTAGCACCCATTGCTTCACCAGCTTTAGTTGCTGAAGCAATGTTTCCGTGGTATTTAACTTTAGCTTTAGGATCAAGTGTTGAAACACTTGCCAAAGTGTGTCCTGTTGTATCGTCAATTAGTTGTGCATAAAAGTTTGTATGAGATTTGAATACGCAAAGACGTGGTTTAGTAGCAGTACCTGAAATACTTTGACGTTCACGCAAGTGTTTAACTTGACGTGCTTGATTACGAGATAATGTAGCCATAATATTTTTTCCTTTCAACTAAACTACTATTTAGAAGCAGTTTTTCCTTCTTTAATACGAATGTATTCGCCTTTGTATGAAATCCCTTTACCTGAGTATGAGTTAGGACGACGAACATCATGCACAACAGCAGCAAATTGACCAACTTTTTCTTTGTCAATACCACTAATAATGATTTCAAGAGGTTTTGGAACTTCAACTTTAACACCTTCTGGTACACTTAGACTAACTATGTGGCTGTAACCAACAGTTAATTCTAAAGTTTGACCATTAAGTGCAGCTTTGTAACCAACACCTTTGATTGCTAATTCTTTTTTGAAACCTTTTGAAACACCTTCAAGCATGCCTTGAATTAAAGCATTTGTTGTTCCGTGTAATTGTTTTGTGTGTTTTTCTTCATTAGCACGGAGGGTTGTAATTTGATTATTTTCAATTTTAATTGCAATAAGTGGGCTGAATGTTTTTTCTAATTGGCCTAATGAACCTTTAACATTTAAGGTTGTACCATTTAAATTAACTTCAACACCTGCAGGGATATTTAATACTCTATTTCCAACACGAGACATAAAGCACCTCCCTATCAAATGTAGGCAATGATTTCACCACCCACATTTTCCTTACGAGCTTGTTTTTCAGTCATCATTCCTTTTGAAGTTGAAATGATTGCTGTACCATAACCTGATAAAATGGTAGGTAATTTGTCAGCTGAAGTATAAACTTTAAGACCTGGTTTTGAAACACGTTTAAAGTCAACAATTGCACTTTGGTTGTTTTTGTATTTTAATGAAACTTCAAGTACTTTGTCTTTACCTTGGCCTTTTGTAACAAAGTTTGTAATGTAACCTTCATTTTGAAGAATTTCTAGAATTTTAGCTTTTTTATTTGAAAAAGGAATAGCTACAGTTTTGAATTTACGTTGATTAGCGTTTTTGATTCTAACAATCATATCTGAAATTGGATCTGTGATAAACATAATTATCAACTCGCTTTCTTCATGCCAGGAATTTTTCCTTCATGAGCTAAATTACGGAAACAAATACGGCAAATCTTGTATTTTCTTAAAACTGCGTGTGGACGTCCACATAATTCACAACGTGTGTAAGCACGTGTTGAGAATTTAGGATGTTTCTTTTGTTTTGCTTTTAATGAAACTTTAGCCATATTATCTATCTCCTTTAATAAATGGAATGCCGATTAATTCTAATAAACTTCTAGCTTCTTCATCTTTATTTGTGCTTGTGATTAATTGAACATCAAGTCCCTTGATGTGACGAATTTTGTCAAATTCGATTTCTGGGAAAATAATTTCTTCTTTAACACCTAATGAGTAGTTTCCTCTACCATCAAATGCTTTAGGGTTGGCACCACGGAAATCACGAATACGTGGCATTGCAACGTTAATTAATTTTTCTAAAAAGTCTCACATACGATCACGACGTAGTGTAACTTTACCACCCATAGGCATGCCTTCACGAAGTTTTCATGAAGCATTTGATTTTTTAGCTCTGGTTTGGAATGGTTTTTGACCTGAAATTTGAGTAAGTTCATTAAGAACTTCTTCAATAGCTTTAGAGTTTGTTACTTCTTTACCTGCTGTCATATTCAAAACAATTTTTTCTAAACGTGGTACTTGCATAACTGATGAATAGTTATATTTTTTCATTAATGCAGGTACAACTGTTTTTGTGTAATAAGCTTTTAAATTCATAATTAAATTTCCTTCTTAGTTTTACGAGCAACTCTAACTTTAGTGTTACCTTTAACTTTAAAACCAACTTTTGAATATTGAGCAGCACCACTTTTGGTAGCTTTTTTAGTAACGATTGCAAGATTTGAAATGTGAATAGGAGCTTCAACGTTCTTGATTGAACCTTCTGTATTACTTTGACTTGGTTTTACGTGTTTAGTTACTACGTTTACATCTTTTACATATGCACGGTTTGTTTTAAAATTTGTTCTAATAATACGACCAATTTTACCTTTATTGCTTCCTGCAATAACAATAACTTCGTCATTCTTCTTAAGTTTTAATTTCATAATTACCTTCTTTATTTATTATTAAAGAACTTCAGGTGCTAATGAAACGATTTTTAAATAACCACGGTCACGTAATTCACGAGCAACAGGTCCAAACACACGAGTACCTCTTAAAGTACCATCATCTTTAATTAACACAACTGCATTATCGTCGAAACGAATATGTGTACCATTTTCACGGCTAATTCCATAAACACTTCTAACAATAACACCTTTAACTACTTGTCCTTCTTTTACTAAACCATTAGGTAAAGCTTTTTTAACTGAACAGATAATAACGTCACCGATATTAGCTGTTTTCTTTCTACTTCCACCTAAAACTTTGATTACACCAACTTCTTTTGCTCCAGAGTTATCTGCAACATTTAATTTAGAAAGTGCTATAACCATTATTTTTCTCCTTCAACTGCGTGTTGTTTAATTTCAACAAGACGGAAGTGTTTGGTTTTTGAAAGAGGACGTGTTTCCATAATTGTAACGATGTCGTTAACTTTAGCTAAGTTTTTTTCATCATGAACTGCGAAACGTTTAACAACTTTAAAACGTTTTGAGTAAAGACTATGAGCTTTATAACTTTCAACTTCAACGATGATAGTTTTATCACCACGTGCTGAAGCAACACGACCTTGAAGGGTCTTACGTGAACTTCTTTCCATTATTTAGCTCCTCCTTTAGATTCGTTAATTTTTAAAACTGTTAATACTTTAGCAATGTCACGACGTACTTCTCTAATTTTGTGACTGTGATCTAATGTCCCAGTTTTGTTTTGGAAACGATAAATAAATAATTGTGCTTTTAAATCGTCAAGTAATTTACGGAGTTCAGCATTATTTTTAACTTTAATATCTTTATAAAGCATTATTGTTGACCTCCTTGTTCTTTAGCTACTATTCTTCAGCTTACAGGTAGTTTGTGACCACCTAAACGTAAAGCTTCTCTTGCAATTTCTTCGCTAACACCTGATACTTCGAACATCATTGTGTTAATTCTAACAGGTGTATATCATTTTTCAGGACTACCTTTACCAGAACCCATACGAACACCGATAGGTTTTGATGTTTTTGAGAAGTGAGGGAAGATTCTAATGAAAACTTGACCTTCACGACCCATATAACGAGTGATAGCAATACGAGCACTTTCTATTTGACGTGCATCGACTCAAGCTGAGCTTACAGCTTGTAAACCAAAGTCACCATAAGCAACTGTATTACCTTTGAAGGCTTTACGTTTGTCATGACGTACTAAAAATGGTTTTCTATATTTAGTTCTTTTTGGTTGAAGCATCTTTTGACCCTCCTTCCAAAATTTCGCCTTTTGAAATTCACACTTTAACGCCGATTACACCATAAGTAGTGTGAGCATTTGCGTGTGCATAATCAACATCTTGTCTTAATGTGTGAAGTCTCATTTCTCCACGTGAATAGCCTTCTGTTCTAGCCATATCAACACCATTAAGACGACCTGAAACTGCAGTTTTAATACCTTTAGCTCCAGCTTTTAATGCATCATTAATAATTAATTTTTGTGCAATACGAAAGCTTTCACGATTTTCAAGTTTAGTTGCAATAGCTTCAGCAGCTAAACGAGCATTAAGTTCAGGATTTTTAAGTAAAACAACTTGTAAATTAATATCAAGATTTTTGTTTTTTAAGTATTTGTGTAAATCAACATTAATTTTGTCGATTGTACTTCCACCTTCACTAACTAACTTATTTGGTGTAGCTGAGTGAATGTAAACTGTTATTTTATTGGCTTGATTTCTTTTGATTTCAACTTGTCCAATTTGGTATTTACGTACTAATTTATCGAAGAATTTGTAAATTTTAGCATCTTCTACTAAGTATTTTCCATAGTTTGCTTTTTCAGCAAATCAAGATGTATTACGCGCTTTTGTAATTCCGTAACGGAATCCATTTGGATTAACTTTTTGTCCCATAATTTACTACTTTCTTTCTTCTAATGTAATTGATAAATGTGATGTACGTTTAAAAATTGAATAAGCTGTTCCTCTTGAATGAGGTTGGTATCTTTTAAGTGTTGGTCCTTCATTAACTAAGATTTCTTTAACAAATAATTTAGATGCATCCATTCCGTGGTTGTTTGTAGCATTAGCAATTGCTGAGTTAATAAGTTTAATAAATAATTCTGAAGATTTCTTGTTTGTATTTCTTAAAATACCAAGAGCTTCACGCACGTCTTTAGATCTAACTAAGTCAGCAACTAAACGTGCTTTACGTGCACTAACACGTTGAATTTCAACATGAGCTTTAGCTTGTTGAACAACTGGTGTATTTTGTGCCATAACTATTTCTTACCTTTGTCAGCACCGTGACCTGAGAAGGTTCTTGTAGGTACAAATTCACCTAACTTGTGACCAACCATGTCATCTGTTACATAAACATCAATAAATTGCTTGCCATTGTGTACTTGGAATGTTAAACCAATGAAACTTGGAAAGATGGTTGAACGTCTTGATCAAGTTTTAATTGGTTTTTTTGGCGCTTTGCCTTCAACAATAGCATCTACTTTTTTAAGTAAATGTTCGTCTGCAAAAGGTCCTTTTTTAAGACTACGAGCCATTATTTAGCATCCTTTCTTCTTCTTACGATAAGTTTGTTTGAAGATTTCTTAGTTTTTCTTGTTTTAACTCCAAGAGCTTTTTTACCTCAAGGAGTAAGAGGAGCTTTACGACCAACAGGTTGTTTACCTTCACCACCACCATGTGGGTGATCCACAGGGTTCATAACTGAACCACGAACTGTTGGACGAACACCTTTATAACGGTTTCTACCAGCTTTTCCTAAATCAACCAATAAATGTTCTTCGTTACCAACTGAACCGATAGTTGCACGGCAACGAGCTAAGATACGACGCATTTCACCGCTTTTTAGACGTAAAACAACATATTTACCGTCGTCATCTTTACCTAAGATTTGTGCAGATGTACCTGCTGATCTAGCAATAATACCTCCACCACCTGGTTGCATTTCAATGTTGTGTACAATTGTACCTTCTGGAATGCTTGAAAGTGGTAATGCATTACCTACTAAAATGTCAGCGTGTTCACCTGAAATAACGCTTTGGCCTACTTTAATGCCTTCAGGCGCTAAAATATACCTTTTTTCTCCATCAGCATATGCTAATAAACAAATATTAGCTGAACGGTTAGGATCATATTCAATGGTCTTAACTACTGCTGGAATATTGTCCTTATTACGTTTGAAGTCAATTAATCTGTAAAATCTCTTAACACGACCACCCTTGTGACGTACTGTAATCTTACCTTGGTTATTGCGACCAGCACTATATTTTAATAGTGTAAGTAAAGATTTTTCAGGAGCATGTCCACTTAAGTTTTCTGTGTAATGTAGAGATGACATATTACGACGTCCATTCGTAGTTGGCTTGTAATGTTTAATTGCCATTTCTAATTTTCTCCTTGCTTAAAAATAGATGAGGAAATTTTTCTTTTAAGCAAAATTTAATCCTCTAATAATTATTTTGAACTCTTCTTAGCAGCTGCAACTTTTTTAGCAGCAGGTTTTGAAGTAGTTGATTTTGTAGTTTTTTCAGCTTTTGAGCTGTCTGCTTTTTTGCTTTCAGCTGCTTTAGCTTTTTTAGCAGCAAGTTTTTTAGCAATTTCTGCTTCTTTAGCTGCTGCTTGTTTTTTATCTTCAGCTTTTTTAGCTTTAACTTTAGCTTCTTTTTTAGCATCTTGAGCTTCAGCTTCATTTGGATAATAAACGATTTTATCTCCTTCAGCTAAAGTAATCATTGCTTTTTTGTAACGATTTGTAAAACCGTTGTAACGACCTACTCTTTTAGGTTTTTTGTCAACTTTTAAAATGTTAACTTTAACAACTTTAACTTTGAAAATAAATTCAATTGCTTCTTTAATTTGATATTTGTTTGCAGCTCAATCAACTTCTCATGTGTATGTGTTTGAACTTTGTAAAATGTTGGTTTTTTCAGTGAGAACTGGTCTATGTAATACTTGAGTAATTTCCATTATTTAATTCTCCCTTCAAAGTTTTTAAGACCTTCATTTGAAAGAACTAATACATCTGCTCAAACAAGGTTTTCAACACTTACTGAGTTTGGTTTAACACATAAAACATTTTGTAAGTTAGCTGTTGATTTGTAAACAGTTTCATCTGTTGTTACCACTAAAATGTGTTTTAAGTTTTCAATTTTTAATGATTTAAGTTTGTTAACAGCATCTTTAGTTGAGATTTTTGACATTTTAAAGTCATCAATAACAACTGATTTTTTGTTTGAAAGTAATGTAAGAGCTGATAAGAATGCAGCACGACGTACTTTCTTGTTAACTTTTAATGAGTAGTTACGTTCTGGAGTAGGACCAAATGCACGTCCACCACCAACTCAAATAGGTGATCTTGTTGAACCTGCACGAGCACGTCCTGTTCCTTTTTGTCTTCAAGGTTTTTTACCACCACCACTTACTTCTGAACGATTTTTAACATCATGAGTACCTTGACGTCTTGATGCTCTATCAGCCATAATGGTGTCAAAAATTGCTTGTTCATAAATTTTTGGTGATGCAAATAATTCTTTTGGTAAGGCTTTATTGTCAAAGTGAAGCAAGATAACTTTTGTAAATTTTTCTTGTTCAAGTTTAGCATCATTTAATTTAAGTGAAGCAACTTTTGCTCTTGAAGATGCATAAGTTTTAGCTTTTCTTTTAGCAGCTTCTGCTGCAGCTTTTAATTCATCTAAAGCTGCTTGTGCTTCTTTTTCTGCTTTTGCTTTTGCAGCAGCTTCTTCTTTAGCTAATTTAGCTTTTTGAGCAGCTGTTTTAGCAGCGGTTTTCTTTTTAGCACTTTCTTCTTTAGCTTCTTGAGCTTTTAATTCAGCTTTAGTAGGTTTTTTAGGTTTTAAGTTTTTAGTTAAATCAATTTCAACTGTAACTTTTTTTCTTGAAGTTGTTTTCTTTTCAGCTGTTGCTTTAGGAGCAGCGGATTTTTTAGTTGTTGTAGTAGCTTTTTTAGCAGTAGTTGTTGATTTTTTAGCTGTAGAAGTTGATTTAGCAGTAGTAGTTTTCTTAGCAGTTGCTACTTTTTTAGTTATAGTTTTCTTTTCTTCACTAGCTTTAGTAGTAGCTTTTTTCTTTGTTTCAGCCATTATTTATCTCCTTCTGCTTTTTTACTTTCTTCAGCTGCTGCAGCTGCATCAGCTTCAGCTTTTTGGATAAGAGGTTCTAATTCACTTGAATGCATACCAACTGTAACTTCAATGTTGTATTTTTTAGCTCTTTCAAGCAATTCATTCATTTTAAGAACTTCTTTAATATCAACAAGTTTAATTGCATCATGATTTGGTAAGCCTTTAACAGCTTCACGAATCATAACATATGAATTTTTAGCCCCAGGAATTGATCCTTTAACTAACATAATGTTGTTTGCTACATCAACTTTAACAATTTCAAGATTTTGAACTGTTGTTCTAACAGCTCCTAAGTGACCTGGCATTGTCATACCTTTGAAAACACGGTTACCTGAGATGTCCCCAAGTGATCCTGTTTGTCTAACAGGTTGACTACCACCACCACCACCATGTGATTTAGGTCCAATGTGTTGGTTTCATCTTTTGATTGTACCGGCAAAACCTTTACCTTTTGATGTACCAGTGATATCTACTAATTCACCAGCTTTAAAAATGTTGGCCTTAATAGTTTGACCTAATTCATAGCCTTGCATGCCACGAATTTCTTTAACGTAGCGCTTAGGTGTTGTTTTAGCTTGAGCAAATTGACCTTTAAGAGGTTTATTGCTTCTGTGTTCTTTCTTTTCACCAACAGCAATTTGGGTTGCAACATACCCATTTTTGTCAGCTGAAAGAACTTTAGTAACAACATTTGGTTGCACTTCTATAACTGTAACTGGAACACTAGCTCCAAGTTCAGTAAAGATTTGTGTCATACCAATCTTCCGTCCTAAGATTCCTTTCATAATTTTTTCTCCTTAAATTTTGTTTGTAATTTAACAACCGTCGCTTGTTGTCTAGCACAAGTCGGATTTTGTTATTTTGTTGAGATTTGAACTTCAACACCTGCTGGAAGTTCTAATCTCTTAATTTGGTCAAGAGCTTTATCGTTTGGGTTTAATAAAACGATCAAACGTTGAAAAGTTCTTGATTCAAATTGTTCACGAGATTTTTTGTTAACGTGAACTGATCTTAAGATAGTCACAACTTCTCTTTTTGTTGGAAGAGGAACTGGACCACTTACTTTAACTGAGTTTTTGGTAGCAACTTCAACAATTTTCTTTGCTCCAAAATCAACTAAAGCATGATCAAAACTTTTAACCTTGATATTGATTTTTTTCATAGTTGTCTCCTTTGTTCATATACGAACTTTACTCCACATAAATTACTTGAAATGTCCTCAACAACTCTTCTTGGCATTTCAACAACCTTATGCTTCTATGTAATAGTTGCTTTTCAATTATAAACGAAGACTTAAATTTTCAAAGAAAAATATTAAATATTTTATTATTAAGTATAATTGTTATATAAATAAAACCAGCTAATAAATTAAAAAGTTAATTTTTACTTATTTTTTAGCTTATAAACTCACTTTTTGATTTTTTGTGCAAATTTTAAAAATGTTAATTTTTTAGCCAAAAAAACAATAAGTTATTAAGTAAAAACTTGAAACACCGAAACAAGTTGTTAAAATTATTTTAAAGCACTTTTTTGTGATTTTTGTTATTAAAAAAGTGATTTTTTAAACTATACCACATCTCTATAAAATATAAAGATAAAAATAATTAATATATGTATATAAAGAAAATTTGAATTTTTTTGTTTAGGTGTATAAAAAACAGATATATAATGTAGCTGAAGATTAATCACATTCTATATATAATATTTTGAAAAAAATATTTTTGTTTTATAACAATCTATCATTCTTAAAAATAATTAAAAAAATTAATAATAAAAAGGAAAATAACTATTATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTTTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACCAAGATCAAAATAACATTACAGTTAGCGCCAATGAAAAAGGTTTAGGTTACTATCCAGAAACAATACAAAGATTAATTAAAGGCAAAAAAAACAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAACGTTTATCATGATGATATACACAAATTTGATAGACTCGAGAAAGTTTACTTTTGAGATGGTCAATTAGTAATTGATGGTTACATTAAAGATCCAGAAGTTATTGAAAAAATTAGTTCAGGTGAAGTAGCTCACGAAATTAAAAACAAAAGTTTTGAAGAACAAATTAAAATCATTAAAAAATTATGCAAAGTTAATATTGAATTTCATCCTGATTCTGCTTATGACTACAAAAGTGATGAAGCAACACATTCTCACCCTAATACTTTACACTATGTTTTTGTACGTGTTAAAAAAGACAACCCTGCTGATAGAAGCATGGTTTCTCGTGATATCCAAGGCTTTGAGCCTAATGCTAAAGTCGATCCAAAAAAATGACTAGGAGTTTATGCTTCTGGAGGTGTTCAAGTTAAAGAAGGTGAAATTGATCACGTTGTTGGACATAATGCGGCAGGTGACATTGCTGATAAAAAAATAGGTTCATTTGTATTTCGTCCAAGCGTTAAAGATGAAGGCAAAAAAATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGAGTTGAAAAATCAATCAATATTGTTGGCTGAGGAAGCAACTTTGAAATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGAAACAATATTCAATATCGTCATGACTTTGAAAAATTATCAATTGTCAAAGAAAAGACAAAAGCAAATGATGCAGCTAATACACTTACAATAACATTGAAAATTGAAAATATATTTACTAAAGAAACTAAAGAATATCCAATTACAATTACAGGTTTCTGAAATGACGGAAAAGAGTATAAAAAAGGCTTTTTCGACAATAAAGTACTATTAAAAGACTATATTAATGAAGATGAATATGATTTCGATTTAAAAGATAAATTTTTAAGTGGATTATTTAGTTTCGATAAAATTCAAGACCAACTTAAATTCCTAAAAGATAGAAAAATAATTAATGAAGCAAAAGATTTTGATGGGGCTAATGAAGAATTTACAACATTTGCTTCAAAAGATTACAAATACTACTTTGATAAAGAAAAAACAAACAATTTAGAAAAGGTTAACAAAGATAAAAGAACGTTAGAATTAGTATTAGTTAGAGAAAATTTAAAAAATCCTTCAGATAAAAAAGAAGAGATTATTAAAATACATCAATTTTTAAAAGATTCCAAAGATGAAGTAGGTGCATTAATAAGTGCTTCAGGACATATTAAATATAAAAAATATTTCACTCCAACTAAAGACGCCAAAAACAAAAAAGCAAGCGATTTATATAAAGAACTTGAAAAGAACAATTTTGAATTCACAACTAATTTAACAAGTTTAATGTATAAATGAGGTATCTATTTTATATCAAATAATATTAAAAATAGAGATTATGATGACAGAAGTCACTTACATTTAGTTTTAGAAGTAACTGAAAAAGCAACAAAAAAAACCAATGTTATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGAGAAGATACTGCAAATAAAAATAAAACTTCAGGTCAAAAAATGAAACTTTGAGTAGATTACCTAAAAAATAATAAATTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTGAAGAACACTAAAACAAATGAAGAAAAAAATGTTTATATTCTATTTTGCAAATTAGCCTAAAATCCTTTTCTATCGTTTTTCTAATAATTAATGAGTCATAATTGACTCATTTTTTATATCTAAAAAGCAGCAAAAACTGCTACTTTTTGGATTAGATAAATTATTGTCAATCGTCACATTTGTAGGTTTCATCATGGTTGTAATTTTGGTTATTTGATTGTGAATTGTTACCTGTTGTTTGATTTTGATCTATATTAGATTTGCGTTGTTGGTTCACATTTTGATCTTGTGTTTTTCTATTTTTAAGATATTTTTCAAAATAGCATGAAACCACCAATGACTAATAAAATAATTCCTGAAACTAATGTTCCTATTCTTTGAGTACTATCAAATGTTTTTTATTTAACTCAAGCAGCAATAATTCCTACAAAACCACAAATTACACACAAACCACCAAAATTCAAAAACCAAATTTATCAAAAAAATGATTTTAGAGCACTACAGGTTGAATTTTCAACTTCTTGTTTAAGAGCATTTGAAAATGCATCTTTGAAACTTTTTGCTATTATTTGCTCACTTTTCTCTTCTAATTTCAATTGTCATATAATAAAATTATTTAAATATAACCATATTTATTTCACATAAAAATACTCTAATGAGTATAAATGTATTATTATTTATATGAAAAAACATTAAACTATGTTATTTTTGGTAAAAAAGCCTCAATTTTCACAATTTTTTTCAATTTTTTTAGTAATTTAAGCATAAAAGGCAAGTTTTAATTGAAAGGATAAAATCAACATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCACTTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGATTCGCATATCAAGAAAAGAACATTGCGCAGGGTAAAAAAGCGTTAAAAGATATACTTTTAGAAAATCCAGAATTCAAAAAAGAAATCGAAAATCAAATTTTTGGTAACGAAGTCAAAAATGTTTCTAAATAACTTTAAGCCCTATTTTATAGGGTTTTTAGTTTAAATATAAATTATGGAATTTAAAAAAATTTATTGGCGAAGATAACAAAATTAATTTTTGGGTTGAAAAATTTTCTATGTAACATATAAATATTAATAACGAAATCATATGTAAAAAACAAAAGCAAAAAAATAAATAATTATTATTGCAACATCTATTGCATGATAATATGCATTCTTAGTCCCATCAAATCATTCATGGATACAAGCATCTATTTGTAATTCATCACTAAAATGCATGTTTTCTTTTCTGCATAATCTAACTCTTAGTCTTACTTCGCTAAACAAGCCTAAAAAGTTTTGATAATTGTTTCTAACATCTTCTATTTGGACTTTTAACAATTCAGCAATTTTAATCATTTTTTTACTTTTCTTCTCGTTTTCTTATGGTTAAAACTCCTAAGCTTGTTAATCTGTTAACTAAAGTTTGATAACTAATTTATTTTTCATAGCCTTCATGAGAATAATCTCCAACTTTTATTTCTTTTCAAAAAGATTTGATTGTTGCATCATTAACATTTTTGATTCCGTTAGTTCTATTTTTAGAAACAATTTGAATAGCATTGGTTTTTCTATTTAAAAAATCTCTAATATGGTTTTGTATCTTTGAATAGATATAATTGATAAACCAACTTTTTTAGCAATTTGTCAATTACTCAAATTCATTCAAGGTGCAATTAATTGATAGTTTTTTGTTTGCTTTTTCCAATTTTGCTGGATTAGAACAAAAACTAAATACTAAATCAATAAATACATCCTATTTTACAATTATAAATGTCAAATTGTTTTGTATTGAAGGTATGACAAACTATTTTAGAATTTATATCCCACTTGTTATTTCTTATTACTTTTATATTTTAATAATATAATAATTTTAATAATATATTTTGATTTTTATCAGTAGAATGAGGTCATTAATATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGAGCTAAAGATGGAATTTTTTCAATAATCAATAATCCTGAATTAGTTAGACCTGCTAATATAGATGAAGGATATCCAGGAAATGGATCAATAGTTTATAAATGTAATAATGGAACTACTTTAAGAGTTACTTCATTAATGGGAATTACTTTTAATAAACTATTAGCCCCTTGAACTCAAGAATATGCCAATAACTTTTTTGATACTATAGATAACATCATTGAATATGGAGAAAAAACTGATTTTCACTTTGTTGACTTTCACGCTGAAACAACAAGCGAAAAATATGTTTTAGGTCTTTATTTAGATGGCAAAATTGATGGTATTTGTGGCACTCACACACATGTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAAAAAATGATATAACTAGTTCAAGCTTTACATAGGTAAAGCTGCAAGTTAGTCAAAGAGCTTGGCTATTAGACTAATATATGTGTTACTAATGAACAAGACAAATGTGCTTGAATCAATGGACCAACATTGAGGGTGTAGACTCTTGCATTGTATAGTAAGTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAATCCTTTAATAAAGCTATGGCTTTTATTTTGCATAATGTGATTTTTCACCAACTTTGACATTAAAACAAATTTTGTATTCTCCTGATTTTTCTATTGTTTTATAATCACTTACAAAAAATCTATTTGAATATTTTTCTAAAGCTGTTTGTAATAAAATTATATTAAAAATGCTACTTTTACTTAAAATTAAAAAGTATTCATTAATTTGATCGCTTGATTTTTTAATTTTTCTAAAGCTATTTCTTTAGTTACTTTTGAAGTATCTCACGTCCCTTTCCTCCGAGTCTCCTTTTTTCTTAGTGCCACAACCTTCACTAATTGTTGGTAATAAAATTGTTGAAATTGTAGGTGTTAAAACTGAGAATTTCAATCAAAAATTGTGCTTTTTATTTTTATTTTTCGTTGTACTTTTTTCTATTTTTTTACTTTAAAAATAAATATTATTTTTTTAAAACATTATGTTATATAACGCAAAAGAAAATAAAAAAATATCAGAATTTTAACTAATTAATTCTGATAATTTCTAGTTGCATTTTGTCTTAAACAACTGTTTTACAGTATTTTTTGATTTTATTTAGCTTTTAATTCATTTAACATTTTGTTGTCAAAAGCTTTGACAAATTCTGTAATTAAATCAATTGTTGCTTGAATATCTACTAATGAACAAACGCCTATTGGTGAGTGTAAATAACGTTGAGGTAAACTTAGAGTAATTGTAGGAACCCCACCTTTACCATATTGTAAACTAGCTCCATCAGTACCGCCACCTTCGGCTATATATTTATAAGCTGGAATTTTGTGTTTTTCAGCAATTTTTACTAACATGTTAACTAATTTAGGATCTGTAATAACAGCACCATCTTGTATTAAAAGAGCTACACCTTTACCTAAAACTGGAGTTCCTTTAATACATCCTGTCGTATCATGACTTGCTCCTGTATCAATTGCAAAAGCAACATCTGGTTCAATAATTGAAACTGAAGTTTTAGCACCTCTTGTTCCAATTTCTTCTTGTACTGTACCAACAATATATGTTTCATTTGGTAAATCTAAATCTTTAATATTGTTTGCTAAAAAGTCAATAACTGTAACACCAGCTCTATTATCCATTGCTTTACCAGCAATTAAATTATTAGGCATATCAATTGCTTCACCTGACATATAAATTCTGTCGCCTACTTCAATACCTCATTTTTCAGCTTCTTCTTTTGAATTGAATCCGCAGTCAACAAACATTTCTTTTGTTGTAGGAACTTTAGCACGTTTTTCAGCTTCCATAATATGAATTGATGTATGACCAAAAATACCAAAAATTTCTTTATTGTCTCTATTTGTTACCACACGACATTTTGTCCCAATAACAGCAGCTGGTCAAACACCTCCAACAACTGAAACTAATAAGTTACCATTTGGTTCGATTGATCTAACTAAATAACCAACTTCATCCATGTGTGCAGCAATCATAATTTTTGGTGCATTTTTAACTTTTGTTTTATTTTTAATAATTAAAGAACCAAAACCATCTCTTGAATATTCAAAATTTTTTGAAGCTGTATTCTTCTTTAAAGCTTCAACAACAGGTTCTTCATATCTACTCATAGCTTCAATAGCCATGTATTCTTTTAATCTTTTTGCTAATTTATCATATTTAGCCATTTTTAAACCTCCTTAAAAAATTACTATATGAATTATATAACTAATTTATTTTTGCTAGGTCTATTTTAAGGCGGTAAATTTTTAATTATTTTTTGCATCTTATTTATAGGTCTTTAAGAAAATTAGTTTTAAACTAAAACAGCAAATTTTTATATTTTAGTATCATTTTTAAAATCTTTAATAATTTTTATACATTAAACACAAGCATGAATGCTTGTCGCATTTTTCTTTTTTATAAATTTTTCTAAATATTAGTATACTTATTTCACTTCGGATCGCTAATAATACATAGTATTATAATTTATATACCAAAACGAGAATTGTGAAAAAGAGAAACAAACTAAAGGCTTTAACTATTTTATCAACTGCTTTTGCAACAAGTATTGTTGCTTTTGCTGCTTCATGTAATAATGAAGATAAACAAGTTTATTTTACTTTAAATCATAATTACAACCAAAAACAGCGAAACATATAACATTTTGTCAAATAACTATCGATACTATAATGATTTTGGAATGAAATTAGCTAAAGAAGAAAATTAAGTTTAATATTAAACTAAAGATGCAAACTTTGCATAAAAAAATTAACATCTTTTTATGCAAATTATTAATCACTTTTTTCTAAAATGCCTATAAAACTGAGATTTTAGGCAAAATAGACTATTAATTTTTTACTTTTAGAGTCAATTTTTTGCTTGCAAAAATTATTCTTATTTTATGTAGCAAAAACTATATTATTTTGTTATAATAAATAATATTTATTATTTAAATGGGAGGGAGGTTATTATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGAGATAGCTTCTTCAATCCTTTTTCATTGATCTTGATTTTACTTTCAATAGTTTCATTAATTACTGACATAATACTTCCTTTAACTAAAGGTGAAAAATCCGAACCTTGAACTGTGTCCATTATTTTAACAATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGATTATTAATTAAAATCATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGCGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAAAGTTTAAAATATGGATTCTTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAGTGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGCAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTACGATTTTGCTTGTGGAACAATTCCTTGAGATAAAGTCGATAAAGAATTAATTACTAAACCGCGGAAGTGAAATGCTAAAAGCATATTGCGCTTTATGCTTTGGTTTGGACCAGTTTCTTCTATTGTTGATATACTTGCTTTTATTCTTCTTTATTATGTTTTCATTCCTTATGCCCATCCTTCTGTAGCTAAAAATTCAGAACAATTCAAGATGCTATTTTGAACAGGTTGATTCATAATTTCAATGTGAACTCAAAGTTTAGTAATTCACTTTATTAGAACTGAAAAAGCTGCATTTATTCAATCAAGACCAGCCTTTATATTATTATTCTTTTCAATATTTGGAAGTATATTAATTACTATTACTCCTTATATTCCTGGCCTTAATACAGCTTTAAAAGTAACACAACTTAATCCTTGATTCTATGCCTTTTTAGTAGGTTCAATTGCTTTATATATTTCACTTGTTTTAATTGTTAAAAAAATCTACATTAGGAAATACAATGAGTTACTATAATTTACTAAAACATCTATAAAACAATAGAATTTAAAAAACGGTTAGCCGTTTTTTATTTTTGCTTTTAGTTAACTCCAGTAGTAACAAATAATTTTTGAAGAGGTTTACATGGTTTTATAGAAAGCGTTAATAAGTAATGTCTTTCGCCAACTCTGACTCAATATAAATGAATAACATATCTATCAGAAACATTTGTAGGATTCCAAAACCAATGGTTAATCATTCTCCATTGTCTTCAAGGAATTGTTGGAATTCTTCTAATGTTCATCTTTGATGAGTTAGTGCAGCTAATCTTGAACTAGTAACTTTGTAATTAAATGTTTTTTTCTTCAGTATCAAAAACAATGTAATTGTCTTTTTCTGATCCAACTTTCATTAATGGAGTAAGACAATCCTTATAATCAGAATCTGAAAGAACATTTATATTTGTTTTTCATTCATTATGATTTGGAACAGAAAAATTGAAAAATTTCTTTTAAAAAATAAGTCTCTTAAGACTTATCAAAAATTAATTTTATGTTTATTAATTGTGTCTGAATATTCTTTAAAATTAGGATAATAAAATTCACCCAATTTATAAAATTCTTCAGAATGATCCATATGAAAGTAGTGGCATAATTCATGAACTATCACATAGTCAATATATTTAGGATCAAGTGCTACTAAAAACAAACTATAACTTAGCTTACTAAAAACATTATATTTTTTGTGGTGTGTACCTCAAGCACTTTTTTTCATTCTAACATAAAAATAAGGGTTATTAATTTGCATTAAAGCACTATAAACAAGCTGCTTTTTAGAAATATAATCTTCTAAAAATTTAGTTAAAATTTTGTCAAAAACCTTTAATCTTTTATTTTGATCACTAGCACATTTTATTGAGCATCTAATAGTTAAAATATTGTTTTCTTTATCATAAACCCCATCACTAGTATCGCGATAATGAAAGCTTCATTTTAATAATGTTTCTTTGCCAAAAAAACTAATGTAGTGTTCTTCAACTTCGCTAGTTAAATTTAAAACTATAGCATTATTTAATCTTTTATTTACTTTTTTAAAAGCTTTAATAAGTAGTCTATCTAAATTATAAAAATTATAATCAGCCTCACTAATATGTCTAAAACTAGTGACCTCAAATACTTTTTGGTCACTATTAAATCTAACGATTATATTTTCACAACGGACATTAACTTTTGAATAAACTGGATAACTAACATCATCAATTATGATGTGTTTAATTAAAACTAAATTATTTTTTCTTTGAGTCATTTTTGTTTAAGATCTTTCCAAAACGTTTAATGTCTTTAAGTGAAGCACTTCTATTTGAACTCACTGATTCATTGATTAACATTAATTCATCTTTATTTAAGTTTCGATCTTCACTCATATCTTTAGCCACATCTTCAAGTAAGTTAGCTACTTGATCTTCAGTTGTTACTAAAGCTCATGATTCTAAGTTAGCTATATCAAAAGTAGTATTTGAAGGCAAGATTACCAATGAAAATACTGGGAAGTTAAAGCCACACATTTTGTAAAAGTGTTGAATGTGTTTAAAGTTTTGAATAAGCGGATTTTTAAATTTCAAAACTTTATTGCTTTTGCTTCTTAACTCTAATTCAGCATTAGTAGCTTCACCACTAAGATGGCCAACATAATATTTAACTTCAATAATAAAAACTGCCTTATTACTAATTAAAATACCATCAAGTTCAAATATTTGACTTTGTCCATATTTAAATAAACCACCATTTAAGTATTTGTAGTTTTTATCTTTTGCAATAGTTAGAAGTTTATTTCTAATTTTTGCTTCAAAACGAAAACCAATTGAATTATTGCGTTTGCTTTTAATAAGTTTGATAATTAAAAAAGTTAACACTAGCACAATAATTAATAGTGTTAAAGGAATTCCAACTGATAGTCCAATTATCATTTCTCTACTTGGTTTAATCATATTTATAATTATATTTAAAATTAGAATTTGTAAAGCAAATTAATAATATAATTAAGAAAAGTGAGTAAGGAGGCATTTATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAGCAGCTTTTTGAGAAGATAGTGAAGCAAAAAGCTGAATAACAAAATTAAAGGAAGCAGATTATGTTGTAATTAATAGTCCAATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGATATACTTTTGGAGATCATGTTAAAATGCTTGAAGGAACATTTAACTTTTTAGGGGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATACTTTAATTTAATTATAAAACAGAGCAATTGGTATAAATTGCTTTTTTTATTTATAATAAAAAATCTCTCATTTTTATGAGAGAAATTTTTAATCATGTTTTTTCAAAATAACTTCATTGATAACAAAGAATTTATAAACTTTTTCTAGAATTGCATTAACCATTTTGTAAAAGTTTTTTTCATCTTCAAAATAATATTTAGTTATTTCAACTGATTCATTAATTATTATTTTAGGTTCAATTGACATAAGTTCATGAGTACCATTAATTAAAATGGCTCTAATTAATGGACTAATTCTTTCTCAACTTCAACCTTCTTTTAAAAATTGACTAACTGCTTTTTTATAAAAATCATAGTTTTTTGCTATCTTTTCAAGATGCGAAAATTGTTCATTACTTAAGTTACTTTCTGAATCAAATAATTCATTGACATTGATTTTTTGATCCATTAATTCATAACGGTAAAGAACATTAATTATACCTAAACGAAATTGACGTCTGTTTTGCATGTTTTTATTTTATAACAAAAGTTATAATTGCAAAATATTTAAATAAAAAGAAGCAACAAGTGCTTCACTAAAATTATTCTAATTCTTCAATCGAAACATTGAATTCACCAATTAAATCATCTTTAGGGCCAAATAATTCATATTTGAATTTTTTAATATTGCCTTCAATAACAACTTCATGTAAAAATGGTTTTAAAAAGAATTGTGTGCCATCTACTTTAACAAAAGAGTTAGGAGCTTTTTGACCTTTTAACATTGTCAACGTTGACATTCCTGTAAAAATATTTACTCTTTCACTATTGATTTCTATACGGTTAGATTCACCAATTGAAGGCTCTTTAAAATGATATGTATGATATTGTAATTGCTTTGGATCTTCATCATCTAAAAATAAATCATATTCTGCATCAGCTTCAAAAGAAACTATTTTAGGTTCTTGGTTTTTAATTTTATGATCTTGAATTGAAGTAAATCTTATTTTCATTTTGCTAACCTTTCTTTAATTAAGTCTTCGCCATATAAATAAATTGCTTTAGCTAATTCAGGACCATGTTCAATGTAAGTGGTTGCTAGACGTATTGGCATAAATAGATTTTTGCCTTTTTTACCTGTAATTTCTTTTGTTTGGTCAATTGCAGCTTGAATATTTTCAATAGTAAAAGTGTTTTTATTTAATAAATCTTTAAAAGTTAAAATTACATCATGATTTTCAGCTTCAACAGCTGGTTTTTTATTTGTTGGTTTTAAATATGGCTGCATATCTTCGATTAATTGCGAAGTTGTAAATACACTTTCTTTAAATGTATCAACAAATAATTTGTTTCATTTTGTTGCTTTTAAATTAAGTTCTTTAAGTAACTTAGAAGTTGGTTCTTCTTTTATATATTGTTTAGAAAATCATTGCATCTTTTTGATGTCAAATTTTGAAGGGCTTTTGCTTAGTCTTGCTGGGTCAAATTTTTCAATAATTTCTGCTTTTTTCATAACTTCAACTGTATCTGGTGAACTTCAACCAAGCAAACTTAAAAAGTTAAATACACCTTCAGGATGGTAGCCTTCATTACGATAATCTTCAATAAATTGTTTTAAACTAGTATCACGTTTTGAAAGTTTTTTACCTTCCATATTAGTAATAACAGTTAAGTGGCCAAAGTAAGGAGCTTCTCAATTTAAGGCTTGATAAATTGCTAATTGTTTTGGAGTATTAGTGATGTGTTCTTCACCACGTAAAACATGACTTATTTTCATATCGTGGTCATCAACTACAACTGCAAAGTTGTATGTTGGATAGCCATCGCTCTTTTGAATAACTCAGTCTCCGATATCATTTGAATTAAAACTGATGTTACCTCTAACTAAGTCTTCTCATTCATAAGTTACATCTTTAGGCATACTTAAACGAATTGAGTAAGCTTTAGCTTTTTCTCTTTTGGCTTTTTCAGCTTCGCTAATTTTTAATCAATTACGATCATATCTAAATGAAGCAATACCTTTTTTGTTTGATTCTTCATGTTGAGCTTCTAATTCTTCACTAGTGTCATAAGCTTTATAAGCTAAACCTCTTTTAACTAATTCATCAGCAATTTCTTGGTATCTATCTAATTTTTCGCTTTGGCGATATTTACCACAAGCCTTATTTGGTTTAAGTGGGCTTTCATCTGGAACTATTCCAAGTCAAGCTAAGTTTTCTAATTGACTTCTTTCACCATCAGCTACATTTCTTTTAACATCAGTATCTTCTAATCTGAAAACAAAATCACCTTTAAAGTGCTTAGCATATAAATAGCAAAATAATGCTGTTCTAGCACCTCCAATGTGTAAATAACCTGTTGGGCTTGGTGCATATCTAGTTCTTATTTTATTCATTCTAACCTCTTATTTATGACTTAATTTGTATATTAATTAATATTATATAGAAAATGTTTATTTTAGTATCTAAAAGGCAAAAAAAGAAAATTAAAAAAGATATCAAAGCAGAGTGAACCCTAAATTCCGAACTTTTTGGAAAGGGGTTCACTTTAAATTTCTAATTAATTTTAATTTCATTGATTTCTCCTTATAAATATAATTTGCTAAATTATTCAATAATTTTAATAAAAAAGCCTACTAATTAATAGTAAGCTTCGATAAATTTTAAGCTTTCGAATTAGAATTTAAAATAGATTTGCAGTCGACAAAACATTGCTAATTACATCTAATGATTCATTCAAATTATCAAAAGGTCTGTTTGGAATAATTGGAGCAAATTTTGATAAATTAGGATTTTTAAATAGTTCGTCTTTATTCTTTATTATTCCATTAAGTCCAAGAAAAATTCCTTTTGCAATTTCATTATTATCATAACCACTGATTTTTTCTTTAATTTTTTCTATTGATAAGTTTTGTTTTTTCAAAGATTCATCAACTTTACTTTTGATTTCAGGATTTTCATTTAATTTATCAATCAATTTGTTAATTGTTTCTTTTATATTATTAACCTTGTTATTTATTTCTTTAACTTTTTTAGGATCATTTAATTCTTCTTTAATATTTTTTGCACTTTGATTATCTTCAGCTTTAAGATAAATATTAATTGCACTTGTCATCTTTTTATATTCATTAGTTAAATAATCATTAAAGTTTCCTGTAGGATTAATTGATTTATAAACTTCAACTATTTTTTCATATTCAGATTCTTTATTAAAATACGTAGCTTTAAGTAATGCAAGACCATCTTTAGCTTTAAAATCTAAAATTTTTTCAAGTCCATATTCACTTACGTTATTTGCATCAAAATCACTTTGAAGTGCATTTTTAAATGATGCTAATTGAATAATTTTTTCATATGCGTTTTGACAATTTTGAAATTTTTCTTTTTCACTTATTTCATTATTTGGTTTATTGTTTTTAGGCATCGGACTTTCAAATTCAAAGAATTTTGTTGAATTAACATCTTTTTGATTTTTGCCACATGAAACAATAGAAACTAGCATGATTGGTGAAGTTAACAAGGCTCCAAATTTAAATATTTTTTTTAATTTCATAAAAACCTCCATTTTTAAACACAATTAAATTATATTATTTATTTTACTTTTTTAATTTATCTAATTGTTCTTTTTCTTCTTTAGTTGTTTTTGATAATTCTTTTATTTTTTGTATTGTTTTATTTAAAACATTAATTGAATAATTTCAATTGTTAAGAATTTTTAAAACTTTACTTTTAAAACTCTTGTAAGCTATAGATAAATATCAAGCTTGAACCATTTCTACATAATAATCATTATTGTTAGCTGTATTCATAATTAAATCTAAATAACTTAATTTAGAATCATCTTTTAAATAATATTTCTTAAGCATAACAATAGCAAAACGATTTGTATATGAATTATTATCTCTTAGTCAATCTTTAATATGACTTAAAAAATCTTTTTTATTTTTGTCTTTTAGTTTAAATTCAAAAATATCAACAATAGCTCAATTGTCTAATTTTTTTGCATATTCAACTAAATATTTTATTTGCTTTTTATAATCATTAATTTCTTTAATAAAAAGACCATGAAGTATATTTTCCTCAAACAAATAATGATAGGGGTTTCACATAAATTCTGATACTTTTGTAGTTATTTCTTTATATCATTTTTTAGCTAAATCTTTAAGTATTGGAATTCTAACTCCTAATAATTTTGCACTTTCAATTTTATAAATTCGCAAGCAAAATTCTTTATATTTTTGATCAATATAAGGTATTAATTTATAATAAAGTTTTTCTTCTTTTCTACATCTTAATTCATTCATTTTAATACCTTACTTTTTTTCTTTAAAAATTATACTTTAAATAAAATATTTTTTTGTAATATGTTAAATATTAAACCTATAAAAAATATTGCCAATTTTACTAAAAAGACTATAAAACAATTGAATTAAAAATTATTCTTCAATAAAACTTTAAAAAATATATTTATCATTTAGGGACATAAGTTAAAAATTATGTCCTTTAATTTTTGGGACATAAGGATTAAATTATGTCCCTATTTTTTGGTCAAAAACGTTAGTTTACTTTTATTATTTTCGGGACATAAGAAAAAAATTATGTCCCGAATTCTTATAGGACATAATTTCTATTTCTAATTTATTTCTCAACTTTTTGAGCTTGTTTTACTTTTCTTTTTAAGTAAACTCCATTACCAATTGGAATACCTTTTCATCTTCCTAATTCGCTTGGATCAACTTTTTTTCTTAATGCTCCTAAAATAAATGCGCCAGCAAAAGCACCTACAACAAGAGCTAATAAATATCAATATCATCCTTGTGCAACAACGAATACAAAGATTCCGCCATGAGGAGCAGGAATTGAGCATCCTAATCCAGCTGCCATTCCACCAGCTAATGTACTTGCAAAAATTGATGAACCAATAACTCTCAAGGGATCACTAGCTGCATAAGGAATTGTACCTTCAGTAATAAATGCCATGCCCATTAAGTAGTTAACTTTTGAATCTTTTCTTTCTTTAGCAGTAAATTTTTGAGGGAATAAGTCAGCTGCAATAGCGATTACAAGAGGAGGAACCATACCACCAATCATAACAGCTGCCATAATTCATGGTTGATTGATTCCATCAGGATCTACTGAAGCAACGCCAATAGTATATGCAACTTTGTTAACTGGACCACCCATATCAGCTGCCATCATGCCAGCAAGTAAAGCTCCCATTCCAATTTTAAAGCCAATTGAAATTGTGTTATTTTTAACAACATAGTTTAAACCTTGTTGAATGTAGTAATTTAAATATGAAAGTGGTGTATTAATTACAAAGAACATAATAACAGCAATAAGTAAGATACCAAATACTGGATAAATTAACATTGGTTTAAGTCCATCTAACGATTTAGGTAATCATTTGAAGCATCATTTTAATCCTAATACTAAGAATCCGGCAATGAAACCACCAATTAAGGCTCCTAAGAAGCCTGCATTAGAACCAGTCTCATAAACCGCAAACTGTGTACTAGATGCCATGGCTCCAGCTACCATACCGACTGCTAAGCCTGGTCTTCCTGCAATTGAATGTGCAATAAAACCAGCAAACACTGACATCATAAAGCCAAAAGCAAATCCACCAGCATTTTTAAAGAATTTTGAAAATGGAGTATATTGTCCAAAATCTTTTCCTACTGCTTCTTTTCCTAAAAAAGAGTCAAATAAGAAGGCTAGAGCAATTAGAATACCGCCACCAATAACAAATGGAAGCATATGGCTTACACCGTTCATTAAATTCTTATATATTTGTTTTCCAAAACTTAATTTTTCTTGATTTGTATTTTCATTTGAAGAAGAATCTGATTTATATACAGCTACTTTTTCATCTATTATTTCTTGTATTAATTCTTTGGGTTGTTTAATTCCTTTTGTTACAGTTGTTTGAAGCAATCTTTTACCATTGAATCTTGTGATTTCAATATTTGTATCGGCAGCAACAATGACACCATAAGCTTTTTGAATATCTTCATTTGTCAAAACATTTTTAGCTCCACCTGAACCTTGAGTTTCAACTTTGATTTGATAACCCATTTCCTTAGCTGCATTTTCTAAAGATTCTTGAGCCATGTATGTATGAGCAATACCAGTTGGACAAGCTGTAACAGCTACTATAAATTTTTCACCATTATTAGTTTGTTCAAATTCATTCTGATCAACTTCTTGTTCTTTTGTTTCAATTAATTTTAGAAATTCTTTGTTGCTTTTAGCATTAATTAATTTTTCAATAAATACTTTATCAACTAACATTTTGCTCAATTTAGCCAAAACTTCTAAGTGTAAGTTTTCACTATCTGTTGTTTCTGGAGCTGCAATTAGAAATAGCAATTTAACAGGTTTATTATCTAAAGATTGATAATCAACTCCGTCTTTTAAAACAAGTGCACTTAAGTGAGGTTTCTTAACGAAAGATCCGCGTCCATGAGGCAATGCTAATTCATCGCCCAATCCAGTTGTTGTTTGTTTTTCTCTTTCCAAAACCGCTGTCAAATAGGCGTTTTTGTCAATTATAATGTTATCACCCATCAAATTAACTGCTAATTCAATGGCTTCTAATTTATCTTTTGCTTTTGCATTAATATCAATATTTTTTAGAGTTAGAAGTTCTTTTATTTTCACTTTTGACCCCTTTCAAGTAATTCTAATATTGCACTTTTATTAGCTATTTCTTTATCAAAAGCAGTTGCTGCTCCACAAGCTATTGAGAATTCTAAAGCTTGTTTATAATCTTTATTTTTTAAATAATTATCAATAAATCCAGCAATCATGCTATCTCCTGAACCAACTGTACTTACAACTTTTCCTTTATATGTAGCACTATAAAAGGTTTGATTATCTTCACTAAATAAATAAGCTCCTTCAGCGCCACAAGACACTAACACATTTCTGGCACCTAATTTTTTAAGTTTTTCAACATGACTTTTTATGTCTTCTTTAGTTTTAACTTTTTCATCAAATAATTCTTCAAGTTCTTCTAAATTAGGTTTAATCAAAAATGGTTTGTATTTTAATGTATTAAGTAGAAATTGTTTAGTTGAATCTACTATTATATAAATTCTTTTGTTTTTGAGTTTTTCTAAAATTAATTCATAAACATTTGTGTCAACATTTTTTGGAACATTGCCAGCTAAAATTAAAGTATCATTATCTTTAATATTAGAAGCAATCTTATCTAATAATTGTTTTAAATTTTGATCACTGATTTTAGGCCCATTAGCATTAATAGCTGTTTCTTTAGCTTTGCCATTTAGTTTTACATTAATTCTTGAATTACCTTCAATATTACAAAAATCTGTTTTGATTTTTTTAGCTTTTAAATAATTAATAATATATTGACCAGTAAAACCACCAACAAAACCTAAAGCTACATTTTTAGTTCCTAAATTATTTAATAGTATTGAAACATTAATTCCTTTACCACCAACAGTATAATTTTCACTTTTCGAACGATTAATATCTCCTGTATTTATTTTTCCCTCTAAAGACATTGTATAGTCAATAGCAGGTGAAAAAGTCACTGTATAAATCATCTAACCTCCTCAATTTAATTCTATTTAAAAAACTACAAAAAAGCACAGAAAAACACAAAAAATTACAAAAAACAACAAAAAAATAGGCATAAAAGCCTATAAATTTGTAGAATTTTGTAGAATTTTCCACATTTTCTCACATTTTATACTTCTATAATGTTCTCAAGATTTAGATCTTTTTTCTTGTAATTAGTTATCAATGTAATGTTTTTATTATTGGTTGCAAAAGTATATTTAAATTCAGAATTTATTTTCGTTTTGTCTAACAAAATATAACTTTTTTTGCATTTTGAAATGACCTCTTTTTTAAATAAACTTTCATTAATTTCAGGTGTTGTAAAACCTAGATTATTGCTATATCCATTTGCTCCAATAAATCCAATATCAAAATTAAAGTTTTTCAAATTATCTAAAGCTAATGATCCAACAACATTAAATGTATTACTTTTTAATAATCCACCTATTAAAATAGTTGTGACGTTTGGATGACTTTTTAATTCTGTAGCAACATTAATTGAATTTGTAACTATAGTTACATTCTTATTATTAATTAATTTAGCTAATTGATGAGTTGTTGTTCCTGAGTCAATAAAAATTAATAAGTTATCTTGTACAAATTCATTTGCTTTGGTAGCTAATTTGATTTTTGCATCTAATTCAATATTACTTCTTGATTCATATTCAACATCATTTGCTGAAGAATTATTATTTAATGAATAAGCTCCGCCATGAACTCTTTTTAATAATTTTTGTTTATCTAATTCAGCAACATCTTCGCGTATTGAAGAAATGCTTGCTTGAGTTAATTTTGCAAGCTCTTTTAGTGAAGCAGCACCTTTTGAATTTACTTCTTTTAAAATAACATCTAGTCTTTGTTCTTTAAGCATAATTTTCCTTTATTTATATTTTAATAAATATATTTAAAAAATAAAGTTTTTAACTTTTAAACTAATTATTAAACATTTCTTTTAATTTTAGGGACATAAGGATTAAATTATGTCCCATTTTAAAATCTACAATTTGGAACATAAGAAAAAAACATGTCCCTAAATTGTTTAAGGACATATCTATATTTTTATTCATTTTAGGACATAAGAAAATTTATGTCCTTCTTTATTATTTGTCTTCTTTTTTAATTTCTAAGAATAATTCATCTAATTGTTTTTATCAACACTGCCTGGAGCTCCTGTAAAAACATCTTGGTTTTTAGCATTCTTAGGAAATGCATAACATCACGAATTGTTTTTTGACCTGTAATAATCATTGTCAATCTGTCAAAGTCCTAAACCAATTCCACAGTGAGGAGGAATACCATAATCAAAAGCTTTTAAGAATCAATCAAATCTTGATTGTTGTTCTTTTTCACTCATACCAATTAAATCAAACATTTTCTTTTGTGTTTTTTTATCAAATATTCTTGCTGAACCTGAACCTAATTCAAAACCATTTAAAACTAAGTCATATGAAAGCGCTCTAACATCCTTCATTTCAATTTTGTCTAATTCTTCTAGTGAATGCGCAAATCTAGTAAAAGGGTGATGGGCTGCTGCTCAAGTATTATTTTCTTCATCATATTCAAACATAGGTCAGTCTGTAATTCAAGAAAGATTTCATTCCTTTCTTGCATAATTAAATCTTTCATTTAATTCAACTCTAACTGCACCTAAAGCTTGAGATGCGTGATCATAAGTATTAGCAACTATAAAGTAAGAACCATTTTTGTTTTTGTTATTTTTGACTAATTCTAAAGCTGCATCTTTAACTTTGTTTGCAAAGTTGGTGTGAACTATTTCACCATTTTCATAAACAAAGTAGAATAGAATATTTGCTTTATTTTTCTTAGCAATTTCTTCTAAGTCTTTAAAGTCTTTTTTGGTAATTAAAGTATCTACAAAAAGCATTCTTTTACTTGGAGCATCTTTAATGATATTAAAGTCTGTATTAAGGCAAAAGTCATTGATGTCAACTATTTTATAATCATATCTTAAGTCAGGCTTATCTGTTCCATAATCTCTTAAAGCATCAAAATATTTAACTCTTGGAAATGGAGTCTTGATTTTAATTCCTATTGATTTAAAAATGTGTTTGAATAATTTTTCAATGTATTTTTGAAAGTCTTCAACATTTAAGAATGAAGTTTCAATATCTAATTGAGTAAATTCTGGTTGACGATCTTTTCTACTATCTTCATCTCTGAAACATCTTGCAAATTGATAATATTTTTCAAAACCTGAACACATTAAAAGTTGTTTAAATAATTGGGGGCTTTGAGGTAAAGCTCAAAATTCATCTCTATTTCTAGTAGGTACTAAAAAATCTCTAGCACCTTCAGGTGTACTTTTAGCAAGCATTGGAGTTTCAATATCAATAAAATCATTTTGGTGCATGAATTCTCTAACAGCAAACATAATTTTATCTTTAGTGATAATGTTTTGTTGCATTACTGGACGACGCAAATCTAAGAAACGATATTTCATTCTGGTTTCTTCTTTAACATCAATATCATCTCTAATTGCAAAAGGTAATTCTTCATTTGCTTCAGAAAAAATTGTGTATTTATCAACTACTACTTCAATATCACCAGTAGCTAGATTAGGGTTCTTTTGTTTTCTTAAAACAACCTTCCCTATTATTTCCATTGAACTTTCTTTTGTGACGTTTAAGTCTGTTTTAAAGACACATTGGGTAATTCCATAGCGGTCTCTTAAGTCAATAAAAGTCATTTCTCCAAAACGCCTTTTATTAGCTACTCAACCATGTAAAGTTACTTTTTTACCTTTGTCTTTAGCACTTAATTCATTGTTCTTTATATATTTACTTTTCATTTTTTATTCCTCGTCTTCATCTTCTTCAGCTATTAAATCGCTTAGTAAATTATGTTCTGCTAAAAAGTCTAATAAGTCTGCATAACCATCTTCATTGTATTGGAACATAATACGATCATTATCGCTTAAACTTTTAGCAACAAAGGTTTCACGACCTTGAATGTTGTCATCAAAAATCACAACATCAGCTTGTAATTTTTGAGCCTTATCAAAGATTTTTTTGCTTTTAACTACTTCTTTAATAAATTCAATTTTGCTTCCATAATTTCTTAACTCATTAGTTAATGAAAATAAAATGTCTAGATTTTTTTCACTGGTTGTGGCTACTAAAATGTTTATTTTTTCAGCATCATTATTGTCTTTGTTTTCATCATTAATAATGTCAGCTGCTCGATCTACTCCAAAACCAAAACCGCAAGCATATAATTGAGGTCCACCTAATTCTTTGATTAAGTTCGAATATCTTCCACCGCCAATTATTGTACTTTGACTTCCTGATTCTTTTGAAGTTGAAACAAATTCATAAACAACTTCATCATAATAATCAAGTCCTCTAACTAAAGAATAATCAACTTGATATTTGATATTGTTAAAGTCTAAAATTGAAGTTAATTTATTGAAAAATTCAGTACTATTTTTGCTTAAATATTTATTAATTTTAGGAGCTTTTTTAACAAAATCTTTTTTGCTTTCTTCTTTATCATCAAGAATTCTTAAAACATTATTTTCAAGTCTTTCAAGACTAATTGGATTCAATTGATCTTTATATTTAGCAAAATATTTTTTTAATTCTTCTTGATATCTAGTTCTAGTTTCTTCATCACCAATTGAATTAATTTTTAAAACACATTTAACATCAAGACATTGTAATAGTGAAGCTGCTGCAATAATTAATTCAGCATCATTATATGGATTAATAACTGTATCAACTATTTCCATCCCTGCTTGATTAAATTGTCTATAACGTCCTTTTTGTGGTTGTTCGTAACGAAACATTGGACCATAATAAGCAAACTTTGTATTTTTAAGTGTTGCATATCATTTGTTTTCAACTAAAGCTCTAACAAAACCTGCTGTTCCTTCTGGTCTTAGTGCAATATCACGATCACCTTTGTCTTTAAATTCGTACATTTCTTTTTTAACTATATCGCTACTTGCAACTGAACGACGATAAAGAGTGGCTGATTCAATAATTGGAGTTTCAATTAAATGGAAATTATAATTTCTTGCATAATTAACAAATGCACTTCTAATTAATTCAATCACATTATTTTCTTGTGGACCAAAGTCTCTTGTTCCTTTAATTCGGTTAAACATTTTATGCCTCCTAAAATAAATACTCAATTTAATATTAAATATTATATATAAAAGTTAAATAAATGTTATGTTTTTAGATTTAATAAAGTTAATATTTGCTTTTTTACTAGCTTTTAGTACCTTATTTTTGCAATTTATGTACTTTTGATAAAATATAAAATAACTATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTGCTGCTATGCTAATAGTAATTAGTGGTAGCAATGGCGCTGGTATTTATTTCCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATTTTTTGCTGAATTTTTGGTGCTTTGGCAATTATTGCTATGGCTTTAGCTTTAGTTGAAATTACTAGTGCTAAAAATCATAATTTATCTTTAATAGGATGAAATCATACTTTTAATAATCGGCATGTATTTAATATGTCAAAAAACTTTATGACTTATTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTGCAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCATTTGTTATTGGCACTAAATATGATTGAATGATTTGAACAGGAATTAGTTTATTAATAGTAATTTATTTTTCAATAGTACCAGCTCTTTACACTAAAGCAGGTGATATTCAAAATAAAATTATGTTGGCTATTAAATTTTTGCCTTTATTCTTTATTATCATTATCGGTTTTGTTTTAGCTTTTAGTTCAAAAGGCGGAGTAAATCAAACTCAATGGAATTTACAAGATCAACACTTTGATGTTAGATTAGGAAGTTCAATTGCTTCTTATAAATTTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATTTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCTATTTCAATGAGTATTAATGGTGGCTTATTTGTTAAAATGAAAGATTATTTAAGTAACTGATGAGGCTCAAAAGCTGGCAGAATTATCTTTGGTATTTTAAACATTTGTATAGCTATAGGAATCTTAGGAATCATTAACAATTTAGCAATGTGATTGCCTCGCTATATTGAAGATTTATTAGCTTTAGGCGAATTACCTTTTTGATATAAACTTAGATTTAAATTAAGTAAAGAAAAACCTAAAATTGGTGTAATTTATAGTTTATTTCTTTGTATTCCTTTAATTATTTTGTTTTCAGTTTTTGGAGCATTAGGTTATTTTTCAAATGAAAATTACAATATCTTTAATAGCTTAAATAGTGATAAAACTATTAAATCAGATGCATTAAATAAGTTATATAACTTTACTGATTTAGTGGCTAACTGAATAGCTTTAATTGTTTATACTTTAATTGCTACAGCAATACTTGGTGGCATTAAAAATAGAAAACATAATTTTGTAAAAGTACAAAAGAAAAAATACTTTTTAGTATCGGCTTACTTTATTGTTACAGTTACTTATTTGTCAGTAATAATCACAACAATTATTTCTTTTATTGATTTATTTTTATTGATTGGTTTTGACAAGGAATACTTTAAAACAGTCGAATTGGAAAAATTTGCAAAAGACAGTACTTACATAATTAGAAATGAAAATACATTCTTTAATCAACTGCTTATAGGTAGAATTTTATGTGTTGTTACATTAATAATTTATTTACTTATTTCAATATTGCCAAGTGTTTTTGAAAAGATCTATTTTAAGAAAATGTTTAAATCATGAGACCTTTATTTAGCTTGGAAACAAGAAACACTTGAAAAAGGAATAGTTGTTTAAAAATAAAAAATATCACTTGATAGTGATACTAATAATTGGCTCTCATAATGAGAGTTTTATTTTATTTAAAATCTAATAGTTGATCTCTGTAATACTTAAATTGTTTACTTCTTAATTCTATTTCAGCAGGTAAACCTGAATTAATTTCTTGAGAATATTCTGATAATTTGTCAAGAATTGAAACTATCTTGTTTTGGGTTTCTAAAGGAGGAATAGGTATATTTAATTCCAAAATATTTTTCATAAACGGATGCTTTATACTACCACCTCTAAAATTTTTTCTAATTAAATCAAGATTTAAAAGAAAGTAATAATATAAAAATTTTGTTGCAACTTCATTTTTATTTAGAACTTGTCCAATTCTATTATCTCCCGTAACAAATTTACCTTTATGATATTTTATATTTGCCTCTCCACCTCCGGGAATTAACAACACTTCACCTGAATATATATCTTTTTTATAATTTTTACTATTAATTTTTAAATTTTCTTCTTTGCCTGTGGTAATAATTTTTACATCGCCTTTAGGATCATTATAATTTTCTATTTCTTTATAAAATAAATATTTAAAATTAGATGTTTTACTTTGTTTAAATTTTTCAACTTCTTGGAAGTTTTTATCTCAGAATGTTATTTCTCATAATTTTTTTAATTTGTACTGTTTGAAATTAAAAATTTTTGTTAAAGTGAAATCAAATTGCTTCCCTCTCGCTTCCAACTCCGCTTTCAGCTCCGCTTCTAGTATACGAAATCTCTCCAAGATTTCGACAATTTTTTCTTGAATTTTTAGAGGAATTAAAGGAATTTTTAGTTTTTTCAAAATTTCTGAATTAAGGTTTGATATTGTCCCCGGTGAGGATTGTTGAAGAAAAAATGATTGTGAATAATTAGATAAAAATCAATAATATAAAAATTTTGAAGTTACATTTTTTTCAAAATTTGCTATTAATAACCAGCCATCATGAATACAACCTTTTATATCAGCAATCACTGGTTTTCCAAAAGACATTGAATTAGATAAAATTAAATCTCCTTTATCAACAACAACAGATTTTTTGCTACCTTTTTCGTTGATAAATTGTTTAGTCTTTTTTAAATAAATTTTTCCTTCTTCTATATCTCCAATTTTTATTCATGGATAACCTTCTTTAGACAAAAAAGAAGATATAGGTCTAGGAGAGGCACCTCTCCTAATGCTTGATATTTCGCCCAATGTAACCCACTCATATCCATCTGGACAATACTTATCTAATAACTTTTGTAAATAACTCATTTTCTTCCTTTATTAAAAATATATTTTAAACTATTATTTTATTAATGCCTTCCAATAACCATTTTTATTTGCTCCAACTCTTTTAATAAAACCATGTTTTTTTAAAAAATTAATATTGTTGTCTATTGCTGTTGTGCTTAGATTTAATTTTTCTGCTAATAAAGCTTTAGTAATATGTGGATTTTTAATAATTTCCGATAATATCTTTTTTCTTATTTGATTAAGAATTTTTTTAGCTGTTTCTTCATCTATCTCTTCAACTTCAATTTCAAATTTTACAGCATTAATTCTATTAAAAGGAATTGTAACTTTAATAAATCCATCACCAAAATAAATAGCTTCTTTGCCATAAACTTTTACTATTGCAGGTACTCCACGTCCTGATTTTTCACTAATTCTTAATTGAAGAAAAATTTCGGCTAACTTTTCATTAACTGGTTTTGATTCTCCTTTGTAAAAACCTTCTATTGTTTGTTCAGGTCCTAAATTTCCCATAGAATCAATTTCAATTCTATCTTTAAATATAGAAATCATTGGAGAAGCTTTTGAAGTTCATTTGTTATGTAAGATGGCATTAATTATTGCTTCCTTGTAAACATTAGAATCAAATAAAGGTACTTCTTTTCTTGTGAGAACTCTGTTTCTTTCATCAGCTTGATAAATATTTACTACATTTCCAAATCTAATCAAATCATCTAGTGTATAAAGTAAATTAGTAAATCCAAATTCATCTACTGTATATAAAGGGGTTGTTTTGTCATAACCTCCAAAAATAGAAACTCTAACTGGAAATCCAGAATTATCTGACAAAAGCTGAGCAAGTAAGTTATACTCACCTTTTTCGGTATAAAATTCAAGGTTTTGTCTAAATGTTTCTTCTTTTAATTGAATTCCTCTTGACCCATAAAATCCAAATAATTTATTAAATGTTAAATCTTGAGTATTGGCTTTAGTGTTTTGAATTGTTTCATGACCAAAAATTAAAACTCAAAATAATTTTCTTTCTCTTTCAGGAAATTTCATTAATTTTTCTTTGCTTGATCCAACTCGAATATATCTTTCATTTTTTCAAGCTGTTGGAGTTTTATGAGCTGCAACTATTTTTAAAATAACCAATCTTTTGCCTTGATAATTGAATTCGCTAAATTCAATATTAATTCTTGGACTGAGTTGTCTTTCTAAATAATGAATTAAAGGTTCATTATTTTTTGTATGACTATCATAATTAAATTGTGTACCAACAATTTTATGAGTTTTATCTTCAATACCTCAAACTAAATATCCATAGTCTCTGCCTTCTATAGCACATGAATTTGAAATAGCAGAAATATAAGAGCCTATTTCATCCTCATCAGCTCTATTAACTTTAAATTCAAATCACTCCTTTTCAAATTCATAAGAAGTTAATTCTTTAATTAATTCATTATTTGTCATGTTTTTTTCCTTTTATTTTATCGATTTTATTACCAACTTTATTGCCCACCTTTAGTATATCAAAATTTTAATTAAAAGTCAATTAAATAGTAAGAAATGATTAATAAAAATAAACCGTATTTGCCTATAAAACAGGCTTTTTGGTTTATTTGAATTGATTTTCATATAAATTAAAGTAATAACCTTTTGCTTCCATTAATGATGCATGATTTCCTTGTTCAATAATTTGACCGTCATCAACCACTAAAATTAAGTTTGCATTTTTAATAGTACTTAAACGGTGAGCTATAACTATTGAAGTACGATCTTTCATTAATTCTTCTTGTAAAGCTTTTTGAATAATTTGTTCAGTATTTGAATCAACATTACTTGTGGCTTCATCTAAGATCAATACTTTCTTTTTACCCAACAGTGCCCGAGTAATTGATAACAATTGACGTTCACCTTGACTTAAACTTTGACCACTATTTTCTATTACAGTGTCATAACCTTTTTCAAGTTTTTGAATAAAGTTATGAGCTGAAGTTAAACTAGCCATTTCATATACTTCTTGGTTTGTGATTTTGTCTGAAGCTACTTTTAAGTTGTCCATAATGGTTTCATTAAACATAAATGAATCTTGTAATACAACTGACATTAAGTCGCGTAAGTTTTTCTTAGTAATTTTTGTTAATTCAACACCATCAATTTTGATTGAACCTTCTTCATATTCATAGAATTTGCTAAGTAAATTAATTATAGTTGTTTTACCAGCACCAGTAGGACCAACTATGGCAATAATTTGACCTGGTAATGCTTCAAAAGTTGCATTTTTAAGTTGATATTTACTACTATTTTTATCATATCTGAAGCAAACATTTCTAAATTCAATGTGGCCTTCAATATCTTTTAATTCAATAGAATTTTTGATTTTGGTTGGAAGTTCAAGTTCTAATAAACGGAAAACACGTTTTGTTGAAGCAAGTCCAATTTGAATTGAAAAAATAACATTGAAGAAAACTTGTAAGGTACCTGTATAGTTTCAAAGCAATGATGTATAAGCTATCATGAAACCTGCATCAGCTTTACTAAAGAAACTAAAAATACTTAAAGTAGGGACATTCTTAATATTAAAAATGATGGCAAAGAGTGAGATTAAAAGAACTAAAAGGTTGGTTGAAATAATAAATCAAGGATCAAAAAATTTAATACAAACATCACCTTTGAATGAGTGTCAATAAATATCATGAGTTATTTTTTTGAAATTCTTTTCTGCTGTTTCTTGACGATCAAAAGTTTGAGTGATTTTGCTATTAGTTAGCATTTCTTCAACGTAAGCATTTAAATCACCAAAGTCTTGTTGTACAAGAACATATGATTTTCTAGCTTTTGAAATCATAAAGTAGCCAAGCGAGAAGAATAAAACCGATAAAGGAATTACTATTGCTGTTAAAGTGAAACCAACAAAAGCCATTGCTAATATTGAAAGAATGACATTGAAAATGTTATTTCAAGTTTCATTGAACATTTGAACAAATGAGTTTGCTAAGTTATTTATATCATTGATTAATACTGAAATTAAGTCACCACTTTTTTCTTTATCATAAAAGCTAACTGGCATTTCAATAAGTTTTTCCATTGCTACTTTTCTCATTTTTGTAGCAGCTTTATAAGCTATGGCAATTAATATCCGACTTTGAATAAAACGTACTATTGCATAACAAATAAAAAATATTGCTAAAATTGAGTTTCAAATAATAAAGCCTTTAACATCAAATGCTCCCGGGATAACTTTACCGCTAGCATCGAACATTTTTTGGTTGAAAAAGAGGCTGATAATAACACCAGTTAAAGTGGTTCCCCCAACATAGAAAACAGCATTTCAAAAGCAAATAAATCCACCAAGAATGACTAATTTTTTATCTTTTTTGTCTAAGAAATCATAAACTAATTTAAACAATCTTCAGGTTGAAAAATTCTTTTCTTTTTGTTTATTTTTAGCCATATTGATTATCTCCTTTCTATTGTTCTAGCTGTGTTTCATAGATCTTTTGGTAAAAATCGCAGGTCTTAATTAAGTGACCGTGTTTACCTTGATCCATTACTGCTCCATTAGTTAAAACCATAATATTGTCAGCATTTTTAATAGCTCCAATTTTTTGGCTAATTAATATTGTCGAACAGTCATAATTGTTTTTAATATTATTAATAACTTTTCTAGTTGTAATATTGTCTAATGCACTTGTTGAATCATCTAAAATTAAGATTTTAGGTTTTCTTAATAAACTACGAGCTATTGAAATTCTTTGTTTTTGACCACCGCTAAGGTTTCTAGCTCCTTGAACAACTTTGTGATCTAAACCATCTTCAAATTTATTAACAAAATCATCAGCACAAGCATTTTTAAGTGCTTCACGCATTTCTTCATCAGTTGCATCAGGTTTAGCTCAAAGTAAGTTTGATCTAATTGTTCCAGTGTAAAGTAAAGCTTCTTGGTAAACTATGCCAACAGTTTCATGAAGGTTTTTGGTATTAATTTCTTTAACTTCTTGGCCTCCAATTTTAATTGAACCTTCATCATAAACATAATTATTTAAAAGTAAGTTAATAAATGTACTTTTACCACTACCTGTTGGTCCAATAATACCTAAAGTTTGTTTATAAGGTAATTTAACATTTATATTCATTAGTGAATATGAAGGGCTGGTTTCATAGTAACGAAATGAAAGATTTTGAACTTCTAGATCAAAATTATTTGTTAATTGTTTACCATCTTTAATAAATAAAGGATCTATTTTGGTTTCTAAAATTTCATTAATTCTACCTGCTGAAACACGAGCTCTAAACATTGATGTTAAGAATTGAATGGTCATTAAGAGACCAAAGCTAATTGAAAATAAGTATTCTATAAAAATATTAACTTTAACAAGTGAATCTAAAGTTGCAACTTCAGTATACATTCTATGACCTGCAAAAGCATAGATTCCAATAATTAATAAGTTAATTGAAGCATAAAAAATCGGTACAGCAAAAGCAAAAATCATATTTGTTTTGAATTGCACTTTATATCATCTGTGGTTGTATTCACTAAAACGTTTTTTTCTTGTTTCTTCTAAGTTAAAAGTTTTAATAACTCTTGCTCCTAAAATATTTTCATCAGTTGCTTTAGTAATTTCTTCAACAACTTTTTGTGTTTTCTTTAAAAGTGGATTAGTAGTTAAACCAATAACAAGTACTAGTACAAAAATTACAGGTACAACAGCTAAAACACTAAGTGACATTTTTGGATCGTTTAAAATTGCTAAAACTCCACCACCAATTACTAAAAAGAAACCTCTAATTAATATACTTGAACCATTAACAAGGAATTCTCAAAAGATTGCAACGTCATTTGAAACTCTAGTAATAATACTTTCTGGTTTTAAATCAGCAATATTTTTAAGTGATAATTTTTGAATTTTATTAAACAAAGCATTTCTAAAAAAGCGGCTACTTTGTTCAGCTGGTCACACAATCATAAGTGTTGAAGTAAAAGTTAAAGTGGCTGCTAAAATTGTTTGACAAACAACAGCAATACTTAAATATGTAATTAAATCTTTACATGTTCCTCTAACTTCTCAACGACCATTAAAGAAGGATAATGTCTCAACTTTTGAATAATCATCAACGAATAATAATCTAATAAACTGAGAAATAATATTAGGCAAAAGCAAAGTAAAAATAATATTAATAAAAACAAGCAATGAACCAAAAAGAAATGCTAATTTAATCTTAATTGGTAGCATTTTAATTAACTTAAACATTATTCCTCCTTGCAATATATAATAAATACTTTTTCTATTATAAAACTTTTTAATTATTAATATATATAAAATATATTTAAAATATTAGAATTTATATTAAACTATAATTATTAAATATTTCAAAAGAAATGAGGACTAAATATGAGTGAAAAATCATGTGGATTAGTAATTTTTAGACAATTTACAAAAGGTTGAAAAGTTTTGATTGCTAAGCAAATAAATAATATGTGAAGCTTTGTTAAAGGACATGTTGAAAACAAAGAAAGCGAAATTGAAACTGCACTAAGAGAAGCTAAAGAAGAAGTTAACTTAGAAAATTTTCAAATTTTCAAGGAAACTAGAATGAAAGAAAAATATGTTTTACCTAACGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATTCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGAAGAAAAAAAGAATAAATAAAGAATCCTTTATTAAATACTTGAAAGGATTCATTTTTTAACATTAATTATTATTAAAACAAAATAGGTTTGATTAAGTCTCAAATTAATTTAGAAGTGTCTTGAACTAATAAAGTTGCTTTTTGGAATAAATTATTATTACCGTTTGTTATTGCAATGGATTTAATGTTAGCCTTATTTAAAGCTTCAACTCCAACAACTGCATCTTCAATTCCAACACATTCTTCTTTTTTTAATTTAAGTTGTTCTTGAGCTAAAAGAAAAATATCTGGTGCAGGTTTTCCTTTTTTAACTTCAACAGGATTAACAATAACATCAAAATAATTTATAACTCCGAGTTTTTCTAAAATCAAAGGAGCATTGAGACTTGAAGAAGCTATTGCAATTTTAATCTTATCTTTTTTTAAGTCTTTTAAAAATTGTTCGATGCCTTCTAAAAGGCTATCTTTATTAATATATTTTTTTAAATAACTTAAATATAATTCATTCTTTTTAGTACATAATTCATTGATTTTTTGATCATCAAAATTATGCTTAAATTTCTTTAATTCAAATATTGCAAGTAAAGTATCTCTACGAGGCAAGCCTCTTAATTTTTCATTTTCTTCTTTGCTATAATCAATACCTAATTCATTGACTATTTCTTTTCAAGCTTTGTAATGTAATTTAGCTGTATCGGTAATTACTCCGTCTAAATCAAAAATTATTCCTTTAATCATTTTTCACCTCGAAAACTTGCTTTTCATTTGTAATTTCACAGTCCATACCATTAACACATATTTTTAACTTAGAACCTTTATTTATAGTATTAATGGTAAATTTGTCCTTGTTGATTTCTACTAAAAATGGCTCATTTTTGTAAATTGCTTTATAAACTAAACTATTTCAATTATTTGGCAATTTTGGAGCTATATGGAGCTTTTTATTAAATCATCCTAAACCACCAAAACCAAAGACAATCATTTGTCAGATTGCTGCTAAAGATCCTGCATGAATACCTGCATTTGAAGTGTGCATTCATGGACCAAAGTCAATATTAATACCATATTTAAATAATTCATAAGCTTTTTCAATTTTATTTAATCTAATAGCTTCAATACAATAAGTTGCTGCCGAAAGCGATGAATCATGTGTGGTTATTGCTTCATAGTAATCAAAGTTTTTTTCTCTAATTTCTTTATTATATAACTCAGGGAATAAATAAGTTAAGAGAACGACATCGGCTTGTTTTACTAATTGAGAGCCTAATAATCTATGTCCTTCAGAAGTATTGAATAATTTTTTACCAGCATCTCCAAGCATTTGGAATGGTCTAACATTTTTTAAAGGTAAGGATAAGAATGTATCATTTTCTGCAATAATTTTATCTTTATTAGGAGCTTGAATTTTAAGACCTTTAATGACTTTAGTCATTTTTGAAAAATTAATTTTATAAGGTATTCTTTTTAAAATACAGTTAAGTAAGCCTGGTGTTTGTGCTTGCAATTTGCGATATATTGAAATTGCCAATTTCATATTAAATAAGGCCATTTGGTTGATAAATGCATTATTATTAATGTTTCCTTTATATTCATTTGGACCCATTACATCTTTAATTTCATAACTACCATCTGGTTGTATTTCTGCACGGTTTGTGTAAAAGTAAGCTGTATCAATTATCATTTCATAACCCATTTTTTTCATGAATTCTTCATCATTTGTAAAATGATAATATTGATAAACTGCATAGGCTATATCAGCTGAAACATGGATCTCTTGTCTTCTACTTGCAATCGGTACTTGTTCACCTGTTACAACATCAGTTTGGCCTCAATAAGGACAAACTTCACCATCAGTAGGTCAAGCCATTTCTCAAGGATATTGAGCACCTAATAAATGACTTTCTTCATTTCTTCGTTTTGTTTCGAAAGCTTTATTTCTAGCTCCTTTTATCCCTTTATAACGATATGTTAGTAATCTTTTAACAACACTTGGTCTAGTAAAAAGATAATTAGGATTAATAAAGAATTCAGTATCTCAATATGTGTGCCCTTGGTAACCTTCACCTGAAAGTCCTTTGGCTCCTACATTTAAGTTAGTTGAATTTTTAGGCACAAAATTATTTAAGTGAAAAATTGAAAAGTTTAATGCTAAAGCATCATATTTACTTTGTTCATTACCTTCGATTTTGACATAAAATTGATTTCAAACTTTTTGCATTGCAGCTGTAGAATTTTCTTTTAATGAAGCATATGTATTTTTTAATAAGTAGTTAAAAGTTAAATCTGATTTAGCCAAAACTTCACTATTAGTTAATATTTTTTCTTCATCATCAATTGATGTATGAACTGACATTAATTTTTCTAAAACTAATTCTTCGTTTGCTTTTAATTCATTTTCGATTTTAAAGTAAACTTTGCGGCGATCAGTTTGAATTTGATATTTGTCATTACTGCCTAAAATTAACTTATTATTTTTTAAAAGTCTTGTAAACATGTGGTGCACAACAAAACGATTTGAATTAGTTGTTATTTGTTGCATCTTCAAACTTTCGGTTGTCACACGGTTTTTAAGACCTTCTTCAAAGTGTTGAGTACCACTGTTTGTTACTTGTCCATTAATTCCTGGTAATAATTTAACCTTAACAATTTCATCTTTAGAATCTAAAATTTTAATTGTAATTTTTTGAGCATAAATGTTTCTTATTTCTTGAGAAACAAAACGTTCAAAATTTAATTCAATTGTAGCAAAGTCTCTTTTGGCAATGACTTTACGACTTAAAATTCCATTTTTAATATCTAAAGTTTTAGTATATTGATCCTTTTCATTAACTTCAAAAATTTGATTATTAAAAGAAATAGGAGTTTGAATTAAATCAGCTAAATTAGCTAATTCAGGAACTTCTCCTTTAGATGTTCTATTAAAAATACCATTAACAAAAAAGTCTTCTTTGTTATATTCTTGAACTTCTTCATCAACACTTCTAATACCTAAATAACCATTACCTAGTGAAAAAATACTTTCACTTTTTGCTGTTATATTACGGTCAAATTTAATCTGTGAAATCTGATTATTATCAGGGTCATATTCAATATATTCCATAATTACTCCTTATTTAATTTCTAACATAATCGACTCAAAAGGTCTTAATTTATCAGTAACTTTTTTGTTATCAATATATGTTGATAATATTTGTTTTCCTTGAACATTTTCAACTACTTTTTGATGTTTTGTCATATTAATTAAAATAACTATTTTTTGCTTATTAAATGTTCTTGTAATCTGTATTATTCCACTGTTTTGTAGGTCTATTTGACTTTTTCCTAATACTAAAATGTCATGATATTTTTTAGATCTTAGGGCTATAATTTTTTTGTAAAAATTAAGAATACTATTTTTGTCTTCTAAATTATTTTGAACACTAATTCATTGTGCTCTTTGATTTGTTTTAATTCAAGTTTTTTGGCCTTCATTAAAACCATTGTTTACTGCATAATTATCTCATTGCATCAAAGTGCGGCCTGAGTCACGACTGTTGATATTGCAATATTTAATAAATTCGTATTCACTGTAAATTTTCTTTTTATCTACTAACTCACTAAAGGCATTAAATACGTCACAATCTTTAAATTCTTCTCTTGAGTTAAATTGATTATTTAATAAACCAATTTCTTCTCCATAATAAATACAAGGAATACCTTTTAGCCCAAAAAGCATTAAAGCATGACTTTTAGCACTTTCTTTATGAAATATGTTTTCATCTCCTCATCTAGAAATACTTCTTGAAGTATCATGATTTGATAAAAAATTGGTTGTCATACTTGGTAAAACTTTTGTTGAATGTTGAAATGGTTGTTGTTGATAAACAAACTCTTTGTAATCTCAATTTGAGTCATAACCATTTCTACCTGTTTCTTTTCCTCAACCAATTCATCATCAAGAAAAGTTGTAATAGTTATCAGCTACTTTTTTAGATCCTGTTCCATATTTTAATAATTCTTCTAAATTAATTCCACTAGCTTCACCTAATGTATAAGCATCTTTTTTATCACTAAAAGCTAACTTATTAAACTTAAGAAGCATGTTGTCTGCACCTTTACATCATGCAAAACAAGAATTAGTTAAAGTTTCTTCAAAGTTTTTTGAAACGTGTTTAATAGCGTCAAGTCTAAAACCTCTAACACCTAAGCCATATCAAAAATCAATAACATCTGACATAGCTTTAACTGTATCTTGATGATTTCAGTTTAAATCCACTTGTTCTTTAGCAAAAAGGTGAAAATAGTATTTTTTAACACTTGGAACATATTCTCAAGCTGAACCACCAAAAATACTCTTTGCTTCTTTTTCTTCTTTATTTAATTCTTTTCTTCAAATGAAATAATTGTGTTCAATATTATCTTCTGATTCACAAGCTTTTTTAAATCATTCGTGTTCATTTGAAACATGATTTAAAACAATATCCATGATAATATCAATATTTCTTTTTCTAGCTTCAGTAGTCAAAAGTTTGAAATCTTCTAATGTTCCAAATTGTTTTCAAACACTTTTGTAATCTAAAACATCATAACCAGCATCCACAAAATTAGTGTCATAAATTGGACAAAGTCATATCGCATTTATACCTAAGTCTTGTAAATAATCTAGTTTTTTTGCTATACCTAATAAGTCTCCATCGCCATCATTATTTGAGTCATAAAATGAACGAGGAAATATTTGGTAAATAATTTTATCTTCTAATTTTATTGTTTTCATATCTCTCCTATTTTTTAATTAATATATATGAACAATGCCCCAATATTTCTTTCGTATTAAAGATGCCTATTTTTGAGCAAAATAAAACTTTGTATTTTTTTAAATCAATTGTTTTATAGTTAGATTGGTTAAAATTGTGAATGACTCTTACTTCTTCTTTTTCATTAAATTTAATTGAATAATCGACAACATTTTGATCTTTAATGTCTCAATTAAATTTCAAGTATTTGTTGATTTCTTCACTTGAATTTAGTCTGAAATATTTAGTACTTTGTCTAAATTTCAAGAGTCTAATAAATCAACGATAAAGCTCTTTTTTAATTCAAGGATTTTCCAAATTATTTCATTTTAGTCCGTTAGTATAATCTGTCGTTTTATAAGAATTAAAATGGTATTTATTTTCATCTGAATTTAATTCAAAATAATCTTTGGCATATGAATAAAATGACTTATGAAAATCTTGACCACTTTTATCAGCAGGTTTACTTTGAGCTAATTCAGTACCTGCTAAAAAGAATTGACGTCCTTGTGTTGTAATTTGCAAAATCAAAGCTTGGCGATATTGGTCTAATAAATCTTTTATTTTTAAATCTTTATTTAAAGATTCTGTAATAATACAATCTCATAATGTTCCACCATCATGACAAGCAACATAAGCTAAGTTATTTCCAACATGTTTAGTAAATAAATCATTTGAGTTTTTTGAATACTTAAAATCATAACTAGGTCATTTGTAATTATTAATATTGCCTATTACACATGAACGATATTTGGCAACCTTTTCATTATTTACTTTAGTAATCAATCCTGGTCCAGACCCAAATTCATCTCAAGTAATTATTGACCTAAGTGAATCATTAAAGTAACCAAATGAATGATTATTATCACTAATTCCTTTAACATAAGAATCTTTGAAATCTAAATCACTAAATTGTCAAGCTTCACCATGGATTACTAAATTAGGTTTTATTTTTCTTAAGTTTTTAAAACAATAATCAAGTGTTTCTTTATCAATAAAACTTGATAAATCAAAACGGAAACCATCAACATCATAATATTCAACAAAATGTTTTAGTGATTCATAAATTAGTTTTCTAACCATATATCTTTGACTTGCTAAAGGCGGATAAATAACAGGTTTTACTTTAGCATTATCTCGATAATAGTAACCAGGCAGAATATTATCATAAATTGAATTTATCATCATATGGTTATAAACTACATCTAAAATAACTCCGATTTTACTTTTGTGTGCTTTATCTACAAATCTTTTAAATTCTTTAATTCTATTATAAGGATCACTTGGATTAGTTGAATAAGAACCATTGATACTAAAGTAATTTAAAGGATCATATCCCCAATTGTAATTAGTAGCTCAACCTTTGCCTTCTCCTTTTTTAAGCATTTTTATATTGTTTTCATTTAATGAATAAGTTGAATGAATTGGCAAAAGCTGCACATGAGTTATATTTAAATTTTTTAAATATTTAAAAATATCATGTTTTAATGTTGCATCAAAAGTACCTAATTTTTCTTTTTCTTTTGCTTTCTTTAAACTAGTAAAATCACGTATGTGAAGCTCATAAATGATAGGGTCTACTTGACAATTAAATTCGGTTTTAAGAAGGTGTTTTATTATGCCACATTTTGATTTATTATCTAGATTAACTATTGCGCCTTTTGCAATTTTATCTTCTTTTGCTTCTCAATCAAAAGGAGCTAAAGAATAAGCATAAGGATCTAGTGCAACAGTTTTAGAATTATCTGGATGAGTGATTAAAAATTGATAAAATTTGCCTTCATATTTTTTTTCAATTTTTACAACTCAAACAGTTTCTTCTTTTTCCATAGAAAAACTAATTGTTTCATTAATATCTGAATAATTTTGAAAAATTAATAGTTTTACATCTAAAGCTAAAGGCTGTCAAAATTTTATTTCAATATGATTTTTATGGAATTTTGTTCCTAAATCAGCTTTTTTATAAGCATATTTAGCATCAAAGTCCTTAAAAAATTCTTTAGTTTGTCCTTTTATATTCATAATAATTAAATTTTAAAATTATTTTAAAATAATGTTTCAGAATGCAAAATAATCTTGATGTATCAAGTGTAAAAGTCAATATAAAAAAGCATAAATAAAAAGCACTTTTTTTACAAGTACTTTATTTAATATTTTACAAATATAATTATGATTTTAATGAAAATTCTTGATGGAAAAATCTAAAGTATTTAGGATTAATATAATACTTTCCAGCTACAGAAATGCTATCTTCATCATAGCCAAAAAATGAAACAACTACCAAGCTTTCATATCCAAATTTTTTAACATTTTCATAAGATAATGAATAAACCATATTATTTAAACCATCGCGCTCAATTAATAAGTCGGTTAAAGATTTATTAAAATTAATTTCCTCATCAACTTCAATATTTTTACAGCTTACATAAATTTCAGAAGTTGCTAAAACCTCATTATCTTTATAATAAGTTTTTTCAAAAGTTCTAAAACCTTGAACAAAGAGAATTCTTTTATCTACCATTCATTGAGGTAATTCCTTGCTTTTTATTTCTTTTCCTTCTTGAGCTGTTGCTCCTAAAAGATAGCTAATTGGTTTAATTAAGTTGTGAAAGTTTTCAGCAACAAAATGTCCTCTTTTTGAAATTGAATAGATAGCTCCTAATGCTTCTAATTTTTGATATGCTTTAACAACAATACTTCTTGAACAATCAAAACGCATCATTAAAGAATGCTCACTTGGCAAGATTTTGTTTGTTGGTACTTTTTGAGTTTTAATCAAATCAATTAAATAATCAATAATCACTTGTGTTTTCTTCTTTTCTTGTAAATCCATAATATAAATATTATACACACCTTTAAATTTTAAAAAAATAAAGCTATTTTTTTGTTAACTTATAAATACCACAAGCATATTTTTGCATTATTATTCCTCATGGCATATTTTTGCATTTATATTCATATAATGCTTCAAAGTGATAATTCCTGCTAATCTTTTGAAGAACATTATTTTGACATTTATGATTGTAAATAACAAACATTAAATAATTCTTATCATCATTAAGATCTTTACGGACAATAACATTTGAATAATCTTTACTGTCTACTACTAATCTTGGATCAACTAAAGTTTCTATATTAGTTAAACTTAAAAAAGAATATTCTTTTCTTAGCTTATTAATTAAAGCAATCATATTATAGATACTATTTCCTGGTGCTATTTCATTTTCAACATAATCAAGCGAATTAGATACATTTAAAAATAAGTTTGATTGACAAGCGTCTAATTTCTTTTCGTAAATAGCTGATATTTCTTGATGGTTTTTTCACTTCATTGGTTCTCTTAAACCAGGATCATCAAAGCTTCTAGTTCCTGCATATGCTAATTCATCACCATAATAAATTATTGGTTTAGCAGGCAAAGCTAAAAGATAAAGCAAAGCAGCTCTAATACTGTCTTTTTCATCTTCTGTGATTTTAGGATAGTTTCTAATGTTTTTTAACTTGGCGTCGATAATTGTCTAATCATCTAAAAGTATCATGATTATCTAAAAAAGGAATTCAGGGTGCTTGAAATTTTGTATTTTGGTATTTAGCAACAAGGTTTTGTAAATAAGAAAAATCTAAACGCACATCATCATTATCTCTAAAATGTTCTCATCCATCATAAGTAGAGTCTAGTGCTTTTTGATTATTATAGGTAAAATATTCAAAAGCTTTTAATGCTTCACTTAATCACTCACCCATCATAAACACTTCTTCACGATTTTGCTTTTGAGTGATTTCCTTGCTTGCTTGTCTTAAAAGATGAAATATTTTTGCTTCATTAAAATTGGTTTTAGTTTCGCTTTCAGAAGAAAAGTATTCACTAAAGGCATCATATCTAAAACCATCAACACCTAATTTAGTTCAAAATTTTTGAATAGCACAAAGTTCATTAATTACATCTGAATTATTTAAGTTTAAATCAGGCATACCGCCTCAAAAGCGACCAATAAATTTTTTATTTGTAGCTTTAAAATTTTGATCAATGTTTTTATATCTTTTTCTTAATTTTTCATTATCAACTCTTGCATCTAAATCATTTTTAAGTGGTTTGAAGTTATAATAATTTTCATATTTTTTGTCACCTTTTAAAGCTGCTTGAAATCAAGGATGTTCATAAGAAGTGTGATTAAAAACTAAATCTAAATAGATTCTTATACTTTTTGAATGAGCTAATTTTAGGAATTTTTTAAATGCTTCCATGCCGCCTAATTGAGGTGCTACATCACAGTAATCAATAACTGAATATCCATGATAACTTGATGAAGGATGAATTGGACTAAGTCATATTTGGTCAATACCTAAATTTACCAAATAATCTATTTTATTGATTAATCCGTAAAAATCACCAATGCCATCATTATTACCATCCGCAAAATTGTAAACCAAAATTTGATAAATAACATTGCTGCTTAATTTTGATTTATTAATACTGCAAGTTTCAATTTTTTGATCTAAATTTAAGTAGGTTAAATTTGGATTTTTTATTAAATATTTTGCATCATCTCAAGATACATAATTTTGGTTTGGATGTTTTTTAATGAATTTTTTAAGTTTCATTTTTTTAATAAAGTTCATAAATTTTTCTTTTAATTTGTTTGTTTTCATAATAATAATTTTAACTTTTTTAAATATTAAAATATTTTTTAAATAAAAATCTTGATGTATCAAAAAAGATGCCGAAGCACCTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAATTTTTTTTATAAAAAAGTCACTCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCAATGTTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTCATTGAATTTTGAACTGAAAAGATTTATTAAGAAAATTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTGTAGGCAAGAGTAAAGGCACGCATTGTCATTTCCTTAGTTTTACCGAAAGAGTCTCTAGATATGGATTTTTGGTAAAAATCCATGAAATTTAAATTTGTGTTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATACGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAAACTAATTTTGATGATATTATTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCAATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAAAGATGCCGAAGCACCTTTTTAATTATTATATTAATTGTTTTTCTAAGTCCTTAGTATCAATTTTGCTTTTAGCAATAATTTGGTCATTTTTAACATATCCATATTTTGTTTTAGCAACAATTAACAAAATAAAGTTTGTTAGTATAACGAACATTCCAATTCATGCATAAGTAATGTTCATTTTATCTAAAAATTGATATATAGCATATTCTGAACCGCTTAATTCTTTAAAGTCAATAACTTTGTTAAGTTTATTAGGACTTAGTGAGATTGCAAAAAAGAATAAACAAAAAGAGGTTAATTGGAGAAATATAAGATAAATAAAGTATTTGTCTGCATTACTTTTATTTGAGAAAAGTCGAATTACTGCAATACAATTAATGATTAAAGATATGAAGAGAATATTTATATTAATCATTATTAATGCAGGGAGGAAATTTTTAAGACCATAAGAAGGAGAGTTTGCAAAAGAACTATTGTAAATCTTTTCTTTAGTTGACTTGATAGCAGCAGGATCTACATCTCGAAGTCAATTTAATTCTTCATCTATTCTTTTTAAGAATAAAAAGAGAAAAGCAAACAATATTGCTATTGCAACTATTGTAATAATAATTGATAATACTAATAAAGATTTACGAGCTAAATAATTTGTTTTAGATTTTTTTAATTTTTGATTAACTTTGAACATTAATTAATCCGTTTTGTTGTTTCCGCATCGAAGATATGGATTCTAGGTTCATAAAATTCAATGTAAACTTTTGAATAAAGCTCGTAAGTATCTGAATTGCTTGCTGTAATTGTAAGTTCGATATCGTCATTATCCAATTTAACAGTTACAAGTTGTTCTTTACCAATTAATTCAATATTTTTAATAGTTGCATGAATTGAATTTGATACTTCAGAAGCATGAATTTTGATGTCTTCACTTCTAAAACCAATTTTTAATTTTTTGTCTTTTAATTGTTCCAAATATTCTTTTGGGGCTTTAATTTTAATGTCTTTTTGAGCACTAACAATTTTGCCGTTTTCACATGCTGCTTTGAAAACATTCATGGTTGGAGAACCAATAAATCTAGCTACAAAGACATTAGCAGGTTTGAAGTATAAGTCACGGCCAGTACCATTTTGTTGAATTGAGCCATCATTGAAGACAACAATTTGATCACCCATTGTCATAGCTTCAAGTTGGTCATGAGTTACATAGATACTGGTTGCGCCAAGCATTCTGTGGATATTAACAATTTCACGACGCATACTTTCACGAAGTTTTGCATCAAGGTTAGAAAGAGGCTCATCCATCAAGAACACTTGAGGTTTACGAGCAATAGCACGACCTATTGCCACACGTTGTCTTTGACCACCAGATAAGTCACGTGGTTTTCTGTATAAGTAATCTTCTATTTTTAGAATTTTAGCAACATCCTTAACACGTTTGTCAATAACGTCTTTTCTTTCTTTGGCTATTTTTAAACCAAAAGCAATATTATTGTAAACGTTCATGTGAGGATATAAAGCATATGATTGAAAAACCATTGCAATATTTCTTTCACTAGGTAAAAGGTTATTATATCTTTTGCCATTGAATAATAAGTCACCTTTTGTAATTGAATTTAATCCCGCAATCATTCTAAGTAGTGTAGTTTTACCGCAACCACTAGGTCCTAAAAAGATACAAAATTTACCTGGTTGAATATCTAAATTAATATTTTCAAGTGTATAATTTTCATTACCTTCATATTTTTTAGATAATCCTTTTAGTTCAATATGAGCTCCTTTTTCAATATTTCTAAGCTCACCGATTTCATCTAGCATTTTGTCCAAATTAATTGTTTCAAAGGATAAATCGTCAAAATCTTCATTTTGATTTTGAGTATTATTCAAGTCTTTATTTTTCTTTTTAAATCACATATTTTATCCTTTCACAGCTCCTTCAGATAAGCCACCAACAATGTATTTTTGTAAGTACATAAATAGACAAAAGGCAGGAAGAGAAGCTAGTATTGAGCCAGCTGCATAAGCACCAGCATTTTTATGTTTTTCTTCAGCATTAAGGAAAATATCTAATCCAACAGCTAAAGTTTTTTCTTCGTTACTTATTAAAACAAATTTCGGTAAGATAACATCCGTAAATGGAGTTAAGAATGATCATAGAGCAGTCATAATTAAAGCAGGCATAATAACTGGTAGAAGTATTTTGAAGAATAATCCTCAGTTAGAACATCCATCAACTCTAGCTGAGTCATCTAATTCTCTTGAAACTGTATCTAAATAACTTTTTAGCATAAAGGTATTTGAAGATATAGCTCCACCAGAATAAATAATAACCAGCATTAATATTGAATTAATTCCTAAATCATTCCCCATCCGCACAATAATGTAAAGACAAATAAGAGATGAAGTGGCAGGAATCATTTGCAACATCATAATAATAGTTAATGAGTGTTTTGAACCAGCGAATTTAAATCTTGAATAAGCATAAGCATTCAAAGCAACACAAATGGTTGAGATAACCATAGTTAAGAATGCTAGCAATAATGTATTACCATACCATCTTAAAAAATAGCTTCGTTCACTTGTAAATAAATAAGTAAAGTTGTCAAAGCCAAATTTAAAAGGTGTTATGGCTACAACCCTTTGGTTGTTAACATTTAGTGAGGCCACAATTAGTGAAACCGCAGGGAAAAGCACAATCAAGGATCAACAAATTAAGATTAAGTAATTAAAGAACAGTCAAACTATTTCCATTGGTGTTGGAGGTTTAGTATCTGATAAGTTATAGTTGATACGAACTTTTTTGTTGATAACTTTTTTAGTTGAAATGTTATCAACAATTGAATCATAAATTTTGTTTTCACTATTCATTATTAGTCCCTCCTTGTCATAGATTTAATGAATCCTCTAGCTGCTACAGCGATACTTAATAGAGCTGCAAGAGTTGTCAAAGCTGCTGCAAAAGCTTGATCAGATTCAATTTTTACTGCACCAGTGGCTAGTTTATAGATAAATGAAATTAGAATATCAGTTGCACCTTCTTGATAAGCAGTTGGTTTAGCAAACGCAGGGCCTCCGCCACTAAAGATACTAATAGTTGTGAAGTTGTTGAATGCTCCAACAAATTGACCAATTAACATTGGGGCAATTGCTAATAATAAAGAAGGTAGTGTTAGCTTAAAGAATTTATGATAATTTTTGGCTCCATCAATAGAAGCAGCTTCATAAATATCTTTTGGAATTGCTTGTAAGTTACCAGTAACTAACATGAAGATAAAGGCATAAGCTATTCAAGTTTGAACAACAATTAAGATAGCTCGAGCTGAGGTTATGTTGTTAAGTCATTCAATAGGTCTATTAACAAAATGAAGTTTTAATAGAATCATATTAATATAACCTGTTGTTCCATTAACAAAAGCACTTCTTAAGAAGTACAACATAACGAAAGCTGGAATTGCTCAAGGAATAATAAAGATTAATCTAAAGAATTTTCTTCCTTTAAGACGATTATTATTTGCTAATATCGCTAAAATAATACCGAGTGAAATAGGACAAAGAGTTGAAGCGATTGTTCAAATAAATGTTCAACCAATAATTCTTTCTAGAGCTGTTAGAATTCCATTATTACGATAAATTCATCAAAGTCCTCATTGTTTCAAGCCAACTCAGTGAACTGGTTGAGTAGGGGCTTGATGCATGTATCCATAGTCAGTAAATGAAATTAGAACTGAAGTAATAATAGGAGCTAGAACAATAAATAACATTAAGATTCAACCTGTAATTGAAATCATTCAAGGGAAGCCGCTTGTATTTAATCATCTTTTTGTATATGATCATTTACTTGGACGTGACCCTTCTTCCATTAATTTAGCTGTTCTAAATGCAATAATTGAACAAACTGTAAAGTAAATTAATACTATTGTTAAGATAATAACAGATATGACACCACCAAAAAGGTAACGCCGAGCATCTCTAAATATGCCCATGCTAGAATTGAAGTGTCGAGCTCCTAAGTCAATGAAACTCAGAATCCCTTCCATACCATCAATACCAGTAGTATAAGCTCCGAAACTAAAAGGAATAAAAATTGCATACAATAATGTGGTTACTAAAAGTAAAAGAATCCCTTTAGTTTTTTGTTTGAAGAATATCAATTCTGAAAGTCCAGGGAAAATAAAACTAATTATTGCTGCTAATCATTTAATATTACGTGTACATTCAATTGGTACAGTTTTAACAGCTTCATTAACTTTACTTTCATAAATTTTTGAAACAACCTTCATTTCGGCTTGTCTTCTAAAGAATCAAGTTGATAAAATTTCTTTATTTTTAAGACTGTTAGTTTGAAGTTTTAATTCATATCTTTTTTCATTATAATCAATCTTTGCTTCCATTTTACTATGTTTAAAAGCTTGTTTTGATATTTCTTTATTTTTATATCTTAAAGCTGTTTGCTTAACTGTTTCAAGATATTGATTATAAAGTTGTTTTTTCTTGTTAGAAACTAATTCTTTTTCGCTTTGATATTGTTCTTTGTTTCTAACTTGTTCTTTTAAAATGTTAGAATTTTCATTTGAGCTAAGATTTAAAGAATTTAGTTTTGTTTCATAAATAGCTAAAACTTTTTCATATAAGAATTTTAGTCTTACTTGCTCAGCATTTATTGCTCTTTCTTTTAAGGCTAAAGCTAATTCTTTTTTATAATTTCCTTCATAAGAATATTTTTCATAAATTGCCGATTCTTTGAAATAAAGTTCAGATTTAAGCAATCATTCAAATTCTTTTTTTAGGCTTTTTGTGTTAAAAAAACCAAAGAATAATTTTTTAAATATTTTAGCTAAAGCAGATGTTTTTTTGTCATTATAGACAAGTTGTTTATTTTTCTTAAATGACTGTAAAACAGATTTTTTGGTAATTTTTATAGATTCATCTATATTAGAATCTATAAAGTTATTTTTAGCTAAAAATTCTAAATATTGTTGGTAATTTCTAGTAGCAACTAAATCATCGATTTTCTTTAGTTGTTGCTTGAAATTTTGATTTACAATCTTAATTTTTGATTTTCCATTTTGCTTTATTTTTTTAATTTTTTCAATATTGCCTTTTTCAACACGTTTAATCTCATTTTTGTGTTCTTCGATTTTAGCATATGCTTCTTTTTTATATGCTTTTACTTCTTGTTTTTGTAATTCTGCTTTTTGATTATATTCATATTCTAAAGCAAGATTTTTTTGATTTGTTTCTAATTTAATGTTGTATTTGTTTGCTTTATAAATCTTTAATTCAATAAATTTATTATTTTGATATTTGTGGTTTAGTGAATTACGTTTTTCAATTAATTCTAAACGACGTTTTTCCAATTTTTGATAAAAGTTTTGGAAATAAGATTTTGGGTCTTCTAAAGTATTTAATATTTTTAGTTCTTGTTTATGCAAATAGTTTTTAATTTTATTGATGTCAAAATCAAGATCTGAATTATGTTTAAGATATTTTAGAGTTATATTGAATAAAGCATATTTAGAAAGAATTTTATTTTCTTCTTTAATTGTTTTTTGCTTTAATTCTTCTAATAATTCATTCTTCTTTTCATCAATGTCAGCTGTTAGTCTTAGTGATTTTTGAAATTCTAGTACATATTTTTTAAGAGCCTTTTTTTCTTTTGTTAACGCTTTAATTTCATACTTAATCATTGAGATTTCGTTATCAGCAAACGATATCTTTTTAATAGCATCTTTAATAGCTTTAATTTTATTTGAATAAGCCGCATATAAACTTGAAAGTTCTCTTTTTAAGTTATCTTGTAGTTTTGTTCTAGCTCGTAAAAATAAATTTTTGTTAATCTTCATTTGTGAATTAATTTTATCGATACGCCTATTAACAACATTTCTAACATGATGTTTATAGGCTTTTAAGGTATCTTGTTCTTCAGAAAGAATTGTGTCAAATTCTTCTCCATATCAGTTAAAAAGTTTTAATTTTTCCATATTAAATGTCCTTTATTCATTGTGTACCATTCATAATGGTGCATTCTTCGAGCTAAATGTAATTTGACTGCTAAAAAGATTAGATAAGAAAGTATTATTACACAAAAGATTGTAATAAATACTCCTATAATAATTTCTTGTTTTAATAAAACTCTAGTGCCTCTAAATCAAGGCAAAGCTCCTAAAAGAGCAAATACTAAAATTCATATAATTAAAAACCCATAGCCTGCTTTTAATTTATGCCTTAATAGAACAATGTAAATTAGAAAGCAAATTAATGCTAATGAGTTTGCAATATAGGAAACAATTTTAGTTATTGTCACTTGTTTAGCAATTTGTGCTTTTTCTTCATCGCTAAGACTTGTCTCTATAAATTTAGAACCATAAACAAAAGGAATTAAGACTCAAAAAATCATTAAGACTGTAATTGCTGCTAAACAAGAAACTAAAAAGTAAAAACTAAATTTGTCTATATTTAATTTGGGTTTAATGTATGGATTTTGATCCATTATTCAAATCTAATAACAGCTGTCGATTCAGTTGAACTATTAATTGTGTCAATTTTTTCTTTGTAAATTGCTGTTAATTTTTCATACATCACTTTTTTAAATGCGTCAAGTGTTTTAGTTGTTGTTGCAATTTCAGAATATAATAATTTGTTTCCAAATACAGCATCATTTCATGAACTTCATGCAACATTACCAAATTGAGGATTTGATTGGTCAATACGTCCTGGTTGGTATTGAATTACATTCAATCCACCATCAGCTAATGATTTAGTTGAAGATAATGTTTGATAAATTAAGTCAACTGTTTGATTTTTTCTATATTGTTCAACAATTTCAATAACTTTAGAAGCTGGCACTTTAGTGCTTTTATCTTTTGCTGGAAATTGAAATTCTTTAGTTAATTTGTCACCAAGATTTCTAACTTCAGCTTGGTTAATGTATCTTGCGTAAAGATATGCTTTAACTGCTTTTGTAATAACTTCATCAGCTACTGCTTTTTTAGATAAAAGTTGTGAAACTTTTTTAGCTTCAGCAATTTTTGCATTGATTAAATCTTGCAATTTGTTTTCTTTTAATGCTGTTTTGAATTTTTCGACAACAGCATCGTATTCTTTACCATTGTCACCATCAGCACCAAAAATGATTTTTTCAAGCTTTCTAATATGGAACGAATTGTCACCTTGTTTTGCATTTGCAAATAATTCATTTAAAACATTTGCTTCGTTTAATTTTGTAAAAGCTACTTGTCAAGTTTGGCCATTACCTTGTAAATCCGAAAGTTTTTCCAAACCTAAAATTGCTGCTAATGCATTACGTAATCCAGTACCTTTTTCAGTATATTTATCACGAATATCTTTGAAATTCATGCCTAATTTAGATTCTAAAGTACTGTTTAACACAGCATGTACTGGTAAATTGTTAGCTGAATACATATTTGAATCCGCAAGTGGGTCATCATTCGCTGTATTTTCTCAAGTACTTGCACTTGGAGAATCGCCTACATTTGATAAATTATTAATTACTTGAATTGATGAATCATAATCTCTATTAAATTCAGCAGCATCTGTATATTTCAATTTGCTCATTAATTCTTGTAATTTTGCATTTTGAGCGGCTGAATCTTGATCAACTTTTGTTTTTAATTCTTTTTGTAAGTCTACAATAAATGGAACTTTTGAATCGCTCTTGAAGTATTCATAGAATGATTGAGAATCAAATACACATTCAAGAAATTCTGCAATAGCTTCTTTTCTTGCTTTTGATTTACCATTATTTCTAATGTTTACTAAATATGATCAAGAACTTGAAGCTTGACGGTATGTATAAGCGTCATTTAATTTGCCTGCATTAAAGAATGTTTTGGCATTACCTGATTTTTGAGCTACTTGAACATCTCAAGTACCAATTAAACCATATTCTAGTTTGCCTTCTTTCATTAATTCAAATACTTTGTTATTTACAGCGCCCATGTCATTACTTAATAATGCTTTTAAGTCATCTTGTTTAATATTTCTTTTGCCTCATACTGAAGTAGCATATGCTCCTGATCTAGGATATATGCAGCTTCATATATAGGAAAGAAGAATTCTGACATTAAATCAACTGCTTTTTTGAATTCAACATGGATAGGATTTTCTGTGTAAAGACCTGTTGAAATTTTTCCATTTTTTAAGAAAAGTAATTTCTTCATCATATCTTCAGCTTTGTATTCATGAGCTACAGCGCCTAAAATACCATTACCATATCAGAAGTCTTGGAATCTTAAAAATGCACGACCTTGTTTTACTAATTTTAGTAATGTATCATTTTCAGGTTTAGCTAAGTCTTTTTTAACATCTGCTAAAGATTTTGTTGAAGCAACTATAACACCTTCAGTGTTGTGTCTAATACCAAAAAGTTTTTCAGCTGGTAATTGAGTAATTTTTTCAATACCGACTACTGAACCAAATGATCTAAGTTGTTTCTTTTCTTCTTCAGTTGCTTTAACTTTTTTAGCAATACGATCTAATAATCCTGGTGAAAATTGATCTAAATCAGCAACTGCATTAGATGCAACTAAAGTTGTAATTCTATCATGTGGTGCATAAAAAATATCTGGCACAATTTTTTCATCTGTTGCCCCTGTAGGCATTTCTAAAGCACCTCAAACATCTTTAGCTATTGTTTTAATTTCATAGCCATTTTGGCCTGATTTTGTTTTCTTAAACAATTCAATTGCTTTGTCATAGAATTTTTGTTGAACGCCATCAACTGCAATTAAAATTGTTTTGCTTTCAGTAGTACCACAACTAATTGTAGCTAATGCTGTCACTGGAGCTGCTAAGGTTGCAAAGCCAATTGCTATTTTTTTAAAAGTTTTCTTAAAATTACTCATAATTTCTCCTTGTTAGCTAACTATTCAAAAACGATTAGATTTTTTTCATAAGCAACTATTGAAAAGATAATCGCTAGTGGACTAAAAAAAGTTAAGACAAATATTGTTGCAATAATTCCGCTTCATTTGTAAAATTCGGTTTTATCCAAAATATAGTAAACATGAATGCAAACGAATATTGCAAATATATAAGAAAGTAATGCAGCTGAAAATAAAATTCTATAAGTATAACTTTTTATTTCGCTAACTATGTTTTCTAAATATTCGCCTTCAAAAGTTCATTTATTTTTGCCTAGTAAATACAAAATTAATATTTCTAAAATCAAAATAATGATTTGAGTAAAAACTAATAACATGTATTCAAGAATTTTAGGTAATGATCTTCTTTCATTTGGTCATTTGCCTAAAAAAAGTCATCTCATTCTAGTTTTAAATGAAACTTTTGTACTTTTTTTGTTTTCTAATCTCCTGTTTTTTTGCATAATTAACTAAATAAATTATATTTTAAAATTAAAATATATGCCTTGTCAGTGTGCAATTCTTGATTAAATATAGGAACATTACCCTTGACCACTCAAGATAATAAAAAATTCAAAAAACAACAAATTTTAGTGTAAAAAATTTCTTTTTGCTGTTTTTTGTGCATTTTATATATTCTTATTCTTAATCTAACTTAATCTAATTGTGGAAATTTTCTCATATATGAGATTTTAATTTTTAATAAGTTAATTCAACTAACATTAAACCAGTATTCCCACTAAAAGTTATTTTTTCATTGTTTATCGAAGAATTATTAGAAAAAATAACATTAATTTTAGTTGGAGCATTCTTAAAGACTAGATTTCATTCTTGACCATAAGCTGTATGATAATAAATTGAATTGACTAAAACTAAAAACTTTTTATCTTTTTGCTTTGCTTTAACTGATTTATATCCAAGTCCTTTATAATCATAACCGGGTTTAAAGAGATCATCTAAATTAGCATAAGTATTTTCTTTTTCAGCAAACATTTTTGGATTTTCTTTACGATAATTAATTATTTTTTTGGCTAAATTAAAATTAGATTTTTTATTCTTATGAGCTTGTTCAATGAATTCTCATTCTTGGATAAATTCAAAAAGATATGACTAGTTTCAATACCCGAACGTCTTTCATAAAAATCTACTTGGTTTTTTTATTACTTCATTTAAAAGCTTCTCTTAAAAAAGCATCAGAATGTGCTTGTGGGCCACCTCTCATATTTAATTCATTACCATTATAAAGAATTGGATTACCACTTTGAATTAATAGGTTACTTAATCCATAACTCATTACATTTAAAACTTTAAAATCATTTTGCTTAATTTCGAGATAAAAAGTTTTAACTCCAAAAATCAATTGGTTATTATAGTAGCGCTTGTTAATTCATCTATCTTCATCATGGTTATCTAAGAATGGTAATCATGTTCTAATATGACCTTCAACTTCATGTTCTTTTAAAATTTTTTCATAATCTTTTTTTGAAGTTTCAATAAAAAAACCATTTTTAAAATGAGAACCATCAATTACAATATTTAAGCCAATTCTTTCTTCATAAGGTTTTGTTGTATCATTAATTGCAAAATATTTATGAGCTTTACTTGGTTTATTTCATCATTCACCAAAGAAAAATATATCATCGCTTGATCTTTGATCTTTAGTTTCTTTTATTACTTCTCTTAAGGCTAATCTTTGCTCATTAAATAATTTTCTCTTTTCTAAGCCTGTTGCGTCTTTAAATTCATGTTTATTTTCTGAATTCATAATGTAATAAAAAGCATCAAATCTAAAACCGTCAACTCCTTTTTTAACTCAGAATTTCATAGCATTAATAATTTCTTTTCTTGTTTTGGAATTCTTTAAATTTAAATCAGGCATACCGCCTCAAAATTCAGCAACATATCTTTTATATGAAGACTTTTGATTAGAATCTATATTGTAAAATAAATTTCTTGTACTAGAATCATCAACACCATATTTCTTTTCACAATTATATTGGAAGTTGTAAAAATTATAAAATTTGTCAAATTCGTCTTTGTTTTGAAGACCTGCTTGGAATCATGGATGCTCAAATGAAGTATGATTAATGACTCAATCCATAATAACTTTTATGCCTTTTTTATGGGCATTTATTAAAAATTGATCAAAGGCTTCCATGCCACCTAATTCAGGAGCAACATCTGTATAATCAATTACATCATAGCCATGATATGAGCTGGCTGGATGTATTGGTGAAAGATATAAAGTATCAATTCCTAAATTGCTAAAATATTCTAAATTTTCATTTAGTCCAATAAAGTCGCCAATACCATCATTATTGCCATCTGCAAAAGAATAAATTGTTAATTGATACATAACATTTGAAGGCTTAATAGATTTGTTAAAAGGCACAATAAATTTTGCATTTTTAAATTCACCTTCATAAGACAAATTCTTTATATTTTTTCAACAAATTTTTCATTGTTTCACAATTAATAATCTTTTTTAACACTTGTGCAACTTGCACTTAAACATAAAGCGATAGGTGCAATTAAAGAAAAATATGCTAATTTTTTTCTTGTTTTTTTACTCATTTATACCTTCTTTAATACAAATATTATATTAAAATAAATTATCTTAATAGTACAAGCTTGCAAATCAAATAAAGAAAAATTACCAAAATGCTTATAAAACAATGTTTTTAGTAATTTTAAGTTATTTTAAAAACTTAATTCAACTAACATTAATCCTGTATCACTATTGAAAATAATTAAATATTTTCTATTTTAGAATTATTAGCTGCAATTACATTAATATTAGTTGGTGTGTTTTTAAATTTAGTTTCTAATTGTCAAAATTGTTTGTATGATTTTTTATTGTGTTAGCTAAAACAAGTATTTTATTGACTGCTTTTTTATCTTTAAGATAGTATTCTGTATCATTGCCATAGTTTCTAAAATTATCTTTAAATATGCCATCTAATTTGAATTTTGGATAAACATATTTTGTTAATTCAAAAGCAAGACCATAGGTTTTAGAGATCTCACTTTTGAATTAACAAAATAAATATAAATATAAAATTTCCAGCTTGTGCTGGATTTTGCCAGCTTACGCTGGAGTATTTTCTTTTGCATTTTTTCACTTTCATATGAAAAAAATGATTTTTAAATTAAAATTTAAGCAGACAATTTAAGAAAATGCATTTTTCTTAAAACTTGCCGCCCTCACTTTTGGAAATAATAAAGTAATAAATTTTGATTCTACTTTTATTATATTAATATATAAATTAATATAATTTATTGCTTGCTATATCAATGTCAAAAGCAAATAAATAAAAAAACTAAAAAGCCTGTTTTATAGACTTTTTAGTAAATTTTGAGTAAAAATAATTAATATGAAATTTTGCTATTTTTTTCCACAAATTTTTCTTTTAATAAAATGACCACAGAAAAAATTGGATTCATTATTATTGAGTAAATTGGGATAGTAATAAGGCTTTTTAAAAGGATTGGAATAGTGATTAAGTAATAATGCTCACCAGTATAATTTAATGACTTTCTGCCAGCATAAAGATAATTGTAATAATTAATGTAAACATAAGGCCCAATTAGTCATCTAAAAATGATCATAATAAAAATAATAATACTGAAGACAAATAGATAATCAAATCATCTTTTTTTCACTTTTTTATTCTTAGCAAAAAGCAATTGAAAAATAATAAAAATAAATGCGATAACACTGAAATTAAAATATATTAAGAGCATTCATAAAACTGCATTTTTAGGTAAATATTTTAGCTTCTTAATTCCTTTAATATGATAACCTTTATCATTTCCACTATTTATAACAACATAGAGTAATAAAGCGAAACCAACTGAAATTACAATTATTGGAAAAATAATTCTAAATAAAAAACTAGCACGATAAAAAAAGTAATAAAAGGAGCTTAAAATACAAATGCCTATTGGAATCAATGCATAAAGATAATACCAAGTTCCAATTGTACCAGTTAAAAGCATCATAAAAGTATCAGCTAAAAATGATATTAAAGATCCTTTAAGTGGTCCAAAAAAGATTCCATATAATATAAAAAAAATTGCATCAGTTTCTAAGGGTATTATTTTTAAAATTGTAAACTTAGAAATTATTGCAACAACAAAATGAATTGCCAATAATATACCTGCTAAAGCAATATCTAAAACACTAAGACGAAATGACTTAGCGAAGTGTTGCTTAAACTTTTTGCCAAATGATAATTCATTAAAAGATTTGTCATCTTCATTGGCTGCAATATTATTGGTAAAATTCTCATTTTTTTGCATATTGTGAATTATATGTTAAAAATTTTTATTTGTTATTATATTATATATATTATGAACAAAAAATTAGAATTTTGGAACAGCTGGAATTAGAGGAATTTAGGTTCTGGTGAAGACCGTTTAAATGTAGCTCATGTTCAAAGAGTTATTCATGGTTTTGCTAAATATTTAAAAAACAAATATAGCCACATTAATCCAATTACTGTTGTTATTGGACGCGATAACCGTAGATTCTCAGCTACATTTGCAACCTTAAGTGCTCAAATTTTAAACCAATATGACATTAAGGTTATTTTCCCTCTATACTTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGAATTGGAATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGATGATTCTTGACTTGCACTAAGACCATCAGGAACAGAACCAAAAATCAAATTTTACATTTTTGCATTTGGTAAAGATCAACAAGAAGCTCAAGCAAAATTTGATAATTTTGCAAGAATTATAAAAGAAATAAAATAACCATAATATTAAAGCAAAAGGATTAGCCTTTTGTTTTATTTAACTGTTTTATAGGCTTATACAAAAAATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAACCACTTATGCCTTCAACAACAGTGTAGCCATGTCAATAGTTAATTTTCTTAAAGTACGCTAACACTAAATCTGGTGAAGGTCCACAAGAAATTTCAACTGCAACTTTTTTATATTTAGGATAAATAAAGTTAATTAAGAAGTTAACAACCAATATATAGAAGAATGTTGTAAATTCTCTCATTCCGAAGAATACTCTGTTACCTGAATCTTCAATAATTTGAGTTCCAACACCATTTGTTAAAATTTTTGCATAGTTATGATGTGGTTGAGCAAAACCAAAAATGATTAAGAATGTTATAGTCATTATGATTGAAATAAGACTTAAGATATTGCCAACACTTTTTTGTTTTTTAGTTGAGAAGTAATAAGCAACAATATCTGTGCCGCCAGTTGATCCGCCATTTTTTCAAGCAATAGCAATTGCTGTTCCAGCACAACCTGAACCAATAGCTCCATAAGCAAAAATTGGTCAAGAATTAGGATTTTCTAAAGTTACACTTCCAACTTTTATTTCTTTAGTTCAACCAGGTGCAAAATTAATATGTTCATGTAATCAGTCTAAAACTATTTTATTTTCTCCAATTGGAACTGAAGTTAATATCAAATTAGTTAATATTTGAAATAGCATAAAAGCTAGTGTCATCAAAGTAAAACTTTTCTTAATCTTAAATCCAAATCCTAAAAGAAGCGGTAAGTTAAAAGCTAAGTAAATTAAAGCAAAATAAGGCTTAGTACTCGGTGCAATTAAGTTAATTAATAAAGGAGCTCCACTAAGTCCACTAGGAATAGTTTCACCTCTATTTAAGAATACTTGCACACCAAAGTTAAAGACCAATGCTGATAAAAAGATAAAAACTAAACGACGAGCATAGAGTTTAGCAATTCTTTTCCTAACTTCCTTGTTTTTAGGATTATCTTTTTCATAATTATGACGGACCATTTCAAGACTGTCAAGAGATTTTTCATCAAGAATTTCCTTTTTAGGTTCTTGGATTTGATTTTTTGCTTCAAAAACCATATCTTTAACTTCTTGAATAGCTTCTTTACTAACTTCAAGGTTATGCACTTGTTCTAGTTTGGTAGTATCTTTTTCGTTGTTATTCATAATATTAATTTTATATTAAATATTTATTTTTTTAATTAATATTTTTGTTATATAATAACCATGTTACTTTTGTAACCATTCTGAACAGGTTTAAGAGCTTATAAGCCACCTTAAAGATGAGCCACAAAATTCAGGAGGTATAGCATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGTCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAATCCTAAATCAACTCACAAGAGAAAATTAGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAGGATTATAACATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCAGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAATTTAGATAAATATGCACATTTTTATGATGTGCATATTTTATTTATTTGACTGTTTTATAGGTTTTTAAGAAAAAACAGGACTTGCCTGTTTTAACTTTCTTTAGCAAATTTTCTCTTTTGAAAAATCATTGCAATAATTGAAATAACAATTGAACAACCTGCGACAATAATTGTTAAAATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGAGCTATTGATGTTACAAAGTAAACAATGACTGTAGCTGTTGAAAGAATAAAGAATAAAGCAATTGCAACTAAAGCAATAATTGAGCCACCAATTAATGAAATTGAAGTTGAATTATGAAAAATGTTATTTATAAATTCTTTACAAGCAGCAAATCCATCCTTCATTGAAATAGCAACTGTGCACATATTTCAAGTGCCAAAGAATCCTACTCCAAATTCAGTTGTTGCTGGTTTGGCTTCTTCTTTGATGATCATTTCAGGTTGCATACCAAAAATGAACAATAAACAAAAGCCAACTATAAAAGCTAATAATACTATTAGCGGAATAAATTTAGTTCTACTATACATTATTATTTTCCTTTTTTTAATTACTATAATTATTTTATAGTATATATTTTAATTTTTTAAAAAATAAACAAGACTTTAAAACAAGACCACTATGGGTCTTGTCTAAATTTAGGCAGATTTTATTTCTTTCCTCTGTAACCAAAGTCAATAACAGCACGTCCTAAGAATTCACCTTTTTGAAGTTTTTCAAATACTTCTGAAACTTCTTCTAGTTTAATAACTTTTGTAACTTCTGATTTAACTTTTCCTTCTGCAGCATATTGAAGTGATTCTTGTAAATCAAGTCTTGTACCAACGATTGATCCAGCTAATTCTCTTTCGAACAATACTGTTCAGAATACTGAAACGCCAATGTTGTCTTTACCAAGTTTGTCTTTTGATGGAAGGCCTACTAATACTTGACGTCCTCCACGACGAAGCATTTCCATGCCTTGTTCACCAGCAATTGTAGCAACTGATGTGTTAATTACAGCGTGAGCTCCACCACCAGTCTTTTCAATAAGTTTTTGAATAAAGTTTGGATCTTTCTTAGAGTTGAATGCAAATTCTGCTCCTGATTTTAAAGCTAATTCGCATTTTTCATCTGTTAAGTCGATACCAATAGGTCTATATCCCATTGCTTTAGCATATTGAATTGCTAATTGGCCAAGTCCACCAACACCGATAACAACCATTCAGTAACCTGGACGAGCTTTAGCTCTTTTAATTGCCTTATAGGTTGTAACACCAGCACATACTACAGGTGCTCCAGTAATTAAATCTAATCCTTCTGGAACAATTCCTACATAATCTTCGTGTCCAATTGCATATTCAGCAAATGATCCATCAAATGTGTAAGCTGACATGTTTTGTTTAGGACAAAGTGTTTCTTCACCTTGTAAACATCATTCACATAATCCACAAGCATCATGTAATCATGCTAGACAAACTCTGTCACCTTTTTTAAGACGTGTACATCCTGGACCAAGTTTTTCAACAATACCGATACCTTCGTGTCCTGGAATTAATGGATATTTAGGTTCTACCAATCAGTCATAGTTTGCAGCGTGTAAGTCTGTGTGGCATACCCCTGAAGTTTCCATTCTAATAAGAACTTGTTTAGGCCCTGGTTTTGGAATTGGAACTTCCTTAACACTTCATTGTTTTGGTTTTTCAACAACAAAAGCTTTCATAGTTGTTTTTGCCATAAAATCTATCTCCTTAAAAAAATAATAGTAATTTTTTAATGTTTTATAAAACATTCATGGGAACATTCCCATAATTTAATTCTATATTTTTTTTAAAATAAAATTGCCATTTGAAAAAAATACTAAAAGTTTTCTACAAAAAACAAAGATTTTTATTTTTTTTAATAAATTATGTAAAAAATGGCTAGGTATATATAGGAGGAGAAATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAACAAAAAAATTAATCAAGCAGAAATAGATTCAATACTAAAGGAGTTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACAAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAATTTTTGTCGTCTAAAATTTCATTAATTGCTTGACCAATAGCGCCAGTTAAATTAGAACCTATAACTCTATCGGCAGCTACTTTTGCTTCTTCAGTGGCATTTCCCATTGCAAAACCAATGTGAGCATTTTTTAACATTTCAATATCATTGCCACTATCACCAAAAGTATAGAGCTCATGAACATTAATTTTTAAATAGTCTAGAAGTGAACTTAAACCATTTCACTTAGTTACACCTAAAGGATTAACATCAGTGTAAATTGAGTTTGTTAAGAAAACTTTTTGTTTATCTTTTAATTTATTTTGAAAATACTTTGTGGTTTCTGCTGCAGTAATTAATGGATTTCTTAATGCCATTTGAGTAACTTTACTATTATTATCTTTAAGAACTTTTTCTAAATCTGAATATGGTTTTAAGAAATTTGAATTTTTATCTGTTAATTTTGTTTGATCTTTAACTCATTCTGGTAATATATTTTTATCACTATTTCTAGATCATGCACCATTAAATTCAGGAGTATCAATAGTTAAAATTAAATCATGAGCTTGAGCATAATCAAAAAGATCATTAAATATATCTTTATTAACTTCGCCTAAAACTTTATAATAATCTTTTTCAACATCATAAAATAATGCACCATTTGAACATACAAAATAATTAATACCTTTAATTGATTTTAAAAGTGGTAAAGTTTTTTGCAAACCACGACCTGTACAAATGATATTCGCATGTCCTAAATTATATGATTTTAAAATTGCTTCTTGAGTTTCTGGATGAGTATTAACTCCATCTGTTAATAATGTGCCATCCAAATCAAAAGCAAACACTTTTCTTTTGTTTTGCATAATGCGCTCCTTACTATATTCATTAATTATATTATTTTACCATTTTTTTCAATCTTTTTTTAAAAAAGACTATTAAGGCAAATTATAAACCAGGACAAAAGAATTAGACTTAAATCCCAAAAAGATTTAAGTCTTTTTGTACTAGTTTATTATTCTAAAACATCTGTTTTATAGGCAAAAAATAAAAAAGCATGAATGCTTTAATATTTGAAATATGTGAACTTGTATCAGAATGGTAAGAAATCATATTTTACACTTTGACTAAATGCCACAAATAGAATCAAACCAACTATCAATATAAAGAATAAAATATCTAATCAACCTATTCTTAATTTACGATATCTTGTTCTTTTTGCATAAGGATCATAACCCCTAGTTTCCATAGCATTTGATAAGTCTTCCGCTTTAGCAAAAGAAGTAACAAACAATGGAATAATTAATGTTGTAAATGATTTAACTTTTTCTTTAATTTTTCCATTCTTAAAGTCAACTCCACGACTTGCTTGAGCTTTCATTATTCTTTGTGCTTCTTCAAAAAGAGTAGGAATAAATCTTAACGCAATCGAAATAATCATAGCAATTATGTGAGTTGGAATGAATAGAAGTTTAAATGGAAACATCAAGTCTTCAAGTGCTTTAGTAAGTAATATTGGTTTGGTTGTAATCAATAATAAAGAAGTAACCATAATCATAATGTAAATTCTTATAATTAATGCTCCAGTTCTTGCTAAAGCTTCTAAAGTTAAGGCATATTCACTATGGCCACCTCAGGGTATTATTCTTGTATAGTCCGAGTCAGGTTTTAACTTCATAGTATAGATATTAACAAAGAGAATAAAGAAACCAATAATTAGAGGCATAATCATTAATCTAAATAAAGGATATATTTTTCTTGAACCAATAACAAATGCAATCATTAGAGGTACTAATAAAACAGCTAAAGAGACAAAGTAATTAACAATAAAAGTTAAAACAATATAAATAATATTGATTATCAATTTTAACCTTGGATCAATTTTATGAATTATTGTTTCATGTGGATAATAACGACCAATAGGAGATTTCATTATGCACCTCCTTTCATTCTTTTATTTAAGAATGCAGCTAGTTCATCAATAGATGTAACTTTAGGCACACTAATGCCTCTTTCCTCTAATTTAGAAACGAATTCTAATAATTTTGGAGGTTCCATATTGTTTTCTTTTAAGAATTTTGTATTCCTTAATACTTCATAAGTATTGCCATCTTTAACAACTTTTCCATCTTTAAAAATCAATACTCTTTTTGCTACTTCTAAAACATTATCCAAATCATGTGTAATAATAATAACTGTTTTACCTTGTTGATGAAGTTTATTAAAAATTTCTAAAACTTCTCTAACTCCAACTGGATCTAATCCGGCTGTTGGTTCATCAGCAATTAAAATGTCAGGTTCCATAGCTAAAATTCCAGCTAAAGCAACACGCCTTTTTTGACCACCACTTAAACCAAAAGGTGATTTATTCAAGTAACTTTCATCCAAACCTACTAACTCAAGATATTTTTTAGCTTTTTCATAAGCTTCAGTTTTATGCACACCAAAGCTTCTTGGTCCAAAAGCAATATCATCTTTAATAGTAGATTCAAACAATTGGTATTCAGCAAATTGAAATACTATTCCAACTCTTTTACGAATATCTTTAGATTTCTTAACTCTTTTTTGTCTTGCTTTAACAATATATGAATCAATAAATTTTTCTTTTTCTTTTGTTTTCTTATTTATTTTTTCATTTTCAAAAATTCATTCAATTGAACCATTTCATGGCAAAATAAGAGCATTTAAATGCTCTATGAATGTAGTTTTACCTGAGCCAGTTTGTCCAATTATTCCTATATATTCGCCTTGATCGATTGTTACATTAATACCACTTAATGCTTTAAATTCCATAGGAGTTCTACGACTAAAAATATGTTCTAAATCTTTAACTTTTATTTGCATAGTTGATTTAATAACTCCTCTTCATTATATGTTGATTCAATATTTTCAATTTTTTCACTGAGCTTATAAATAAAAGGTGAATCAATTTTAGCTAATTCAATGATTTCATGATTTTTTAAAATTTCTTTAGGTGAGCCACTTGCAACAACTTGACCTTTAGAAAAAACTAAGCAATAATCAGCTTGAATAGCTTCATCCATATCATGAGTAATTGAAATTAAAGTTTTTTGACGAGTTTTTTGGATTTCTCTAATAATTTTTAAAACTTGACTTTTACCTCTTGGATCAAGCATTGAAGTTACTTCATCAAAAATTATGATTTCAGGATTTAAAGCTAAAACAGAAGCTATGGCAACGCGTTGCTTTTGACCTCCTGAAAGGAATTGAGGTTCTCTTGTCAAATAGTCCTTCATTCCTACTTTTTCAGCTAAATCATCAATAATAGGTTTCATTTCTTTTTGTGGAATGCATTGATTTTCTAAACCAAAGGCAATATCATCTTCAACACTAGCGCCAACAAATTGGTTATCAGGGTTTTGGAAAATAATGCCAATTTTCTTTCTTATTTCTCTGATTGTTTTTTTAGAAATAACTGTTCCATTTATTTCAATAGAACCTGAAGAAGGTTTATACAAAGCAACAAGCAATTTACTGAATGTACTTTTACCTGATCCATTGTGACCTAATATAGCAACATATTTACCATCTGGAATGGTAAAATTAAGCCCATTGAGAGCTTTTGTTTTTGATTCTGGATAAGAAAAAACTAGGTCCTGTACTTTAATCATAAAAATATTATATTATGAAATGATTTTTTTAAAAACCAATAAAATATTTTTTATTTATAAAAAACAAAAATGTTTAATTTTTATTATTTCTAATAATACTAAATTCTTGAAGATATAATTCAACAATTTTTATCTTTTGATCAAGATTTAATTTTAAATAGCCTTTTATTTCTCTATTTCATTTGTTTCTTTTTTAAATTTGAATTGCATAAAAATGAACCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAATGTGCCTTATTGTGTGATTAAAATAATCAAATTATGTATGTTGAATTTAGTCTAAAACAGCTATAAAACTGTCTTATTGGTAAAAATTATTATTTAGTTACTTTAGCATCTTTTTTGCTTTTTGTTGCTTTTGATGCTGTAGTTTTTGTGTCTTTGTCTTTTGATGTTTTTTCTGCTTTGTTTGTTTTAACTTTGTCGCCTTTAACTAATTCAATAATAGCCATAGGTGCATTGTCTCCATCACGTGAAACCAATTTGTAAATACGTGTGTATCCACCATTACGGTTTAAGTAACTTGGAGCAATTTCTTCAAATAAAACTCTTAAAACATCTTTACCATCTGTGTTTTTAATTGTACGTAAGAAACTTGCAGCAATACGACGGTTTGCTAAAGTTGGGTTTTTTGCTTTGGTAATTAATTTTTCTGCATGGCGACGTAATTCTTTAGCACGAATAAGTGTTGTAGTTAAACGTCCGTGGATGAAAAGTTCACTAACTAAGGTACGAATAACACCTTTTCTTCATTTAGTATCACGACTATAAATTTGTTTTGGGTTAGCCATTATTCTTCTCCTTTACTTAGTTCATATCCATATTCTTTTAAACGTTGTTGAATTTCATCAAGTGATTTACGACCAAGGTTTTTAACTTGTTCTAATTCTTCAAGTGACATTTCAGCTACTTGAGAAATCTTTGTTTTACCAATTCTTTTAAGTGCATTCAATGAACGTACCGAAAGATTTAATGAATTGATATCAACATCATTGTCGTTTGTTTCTTGAGTTTGTTCACTTTCTTCTGCAAATACATTTACTTTCATTTCATCAACATTACCAATCAATTTGAAGTGACCAACTAAGATTTCACAAGCTTGTTGTAATGCACTCTTAGGATCAACTGTTCCATCTGTTTGGAATGAGAAGGTTAATCTTTCTTCAATTTTGTTTGATGATGAGTTTAATTCTTCAACTGTGTAGTTAACTTTTTCAATTGGACTAAAGTTACTATCTGTAGCAATAAAAATTCCGTTTTTGATTTTTGATTCCATTCTAGTTGTAATTGAAGAGTTATTAAGTAATTTCTTATTTTCTTCATTAGAAATAAAGCCACGACCTGGTCTTAAATATAATTCAATATTTAATTTGTGTTTACCATTTGCTGATAAGTGAGCAATTTCTAATGATTTGTTTAAAATTTCAATACTTGAGTTGTTTACTTCTAATAATCTTGAAGTAACTTCTCCTGGTTGATCAGCTTTTAATGTTACTTTAATAATTTCGTCATCTTTAACAAATTCTGGGTTGTATTGGAATTTTACTTTTCTTAAATTCATAATAATTGTGGCAACGTCTTCTACGCAACCATCAACTACTTGGAATTCATGATTAACATCTTCAATTTTGATTGCAAATAATGCTAATGAAGTAATGTTTGAAAGTAAAATACGACGAAGAGCAACACCTAATGTTTGACCCATACCACGTTCAAGTGGTTGAAGAGTAAATGCAGTTACATTTGAAGTTGAATTATTTAATTTAGTAACCTTTTGGTACTCTAATTTTGTCATTTTTTCCATAATTATTCAACTCCTTTACTATTTTTTAGCTTCACGTTTAAGAATACGTTTTGGAGGTCTTGTTCCGTTGTGTGGAACTGGTGTAACATCTCTAATTTCTGTTACTGTGATACCGGCAACTTCAATTTGTTTTCTAGCAGCGTCTTTACCTGCACCTAAACCTTTTAATTGAACTTTTACGCTTTTAATACCATGTTCTTTGGCAGCTTCAGCAGCTGCTTGTGCAGCTAAACCAGCTGCATAAGGGGTTTTCTTTTTGGTCCCTTTGTAACCAATAGCACCTGAAGAACTTCATGCAATTACGTTTCCTTTTTCGTCAGCAAAGGTAACAATAGTATTTTGGTTTGTTGAGTGAATGTGTGCTACACCATTTGTTATATTTTTCTTTTTGGTTTTTCTAGCCATAATTAATTCCTCTTCCTATCTATTATTTAGTTGATTTTTTACCTGCAACTGTTTTACGAGGACCTTTACGTGTACGAGCATTCTTTTGTGTACATTGACCTCTAACAGGTAAACCTTTTCTGTGACGAATACCACGGTAGCATTTGATTTCCATTAAACGTTTGATGTTTAAACTGGTTTCACGACGAAGATCACCTTCAGTTGTGTATTCTTTAGCTACTTCACGAATTGCTGATAATTGTTCTTCTGATAAATCTTTAACTCTAAGATTTTCATCAATTTTTGCTTTAGCACAAATTTCTTGTGCTCTAGTTCTTCCTATACCATAAATGTATGTTAATGAGATAACAACACGTTTATTGTTAGGAATTTCAATATTTAAAATTCTAGCCATTTAATTCCTCTTATCCTTGTCTTTGTTTGTGTTTTGGTTGTGAGCAAATTACCCGAATAATTCCTTTACGTTTGATAACACGACAATCTTTACACATTCTTTTTACACTAGCTCTAACTTTCATATAATTCTCCTATAAATCTATTTGTGACGATATGTTATTCGTCCTAAAGATAAATTGTAAGGACTAATTTCAACATCGACAGTATCTCCAGGCAAAATACGAATGTGATTTACACGCATTTTTCCTGAAATGTGAGCTTTAATGAGCATGCCGTTTTCAAGTTCAACATCATATTCATCTGTTGAATAAGCTTCTTTGACAACAGCTTTCATTTTGATTGCGTCTTTTGCCATTTAGATTCCTTTCGTTAATATAACACCTTTGCCATCTTTAATTAAAACAGTGTGTTCGTAGTGAGCTGCATTTAAACCGCTAGCGCTAACAACGGTTCATTTATCTTTTTTAACTTTGATTCTAGCATTTTGTTGTAAAATCATTGGCTCAATACAAATTACCATTCCATCTTTTAATAATGGTCCACTACCAGCATGGCCATCATTAAATACATAAGGGTCTTCATGAACTTCACGTCCAATTCCATGCCCACAGTATTGACTTGGTGTATATAAATTATTTTGTCTTATTACTTGACCAATAGCGTGACTAACATCTCCCACTCTTGCACCAGGTTTAATCGCATTAAAACCTGCATAGAATGATTCTTGAGCCACTCTAATTATTTTTTTATCACTTTCATTGATATTACCAACACCTTTAGTGAAGGCACTATCGCTGTTCATTCCTTTGTAAGTACATCCCATGTCGACTTTAACTATGTCGCCATCTTGAATAATGTAATCACTTGGAATTCCATGGATCAATTCTTCATTTATTGAGATGCAACAGGTTGCTGGAAAACCATATAAGCCTTTAAAAGCTGGTTGTGCACCTCTTTTTACTATTTCATTAAAAGCGACTGAATCCACTTCTTTTAATGAAACCCCTGGTCTTACAAAGTCATAAACTATTTGTTTAACTTCTGCCAGGATTTTGCAGCTTTGAGTAATATTAATAATTTCTTGTTCATTTTTAATAGTTATCATAAGCAGCTTCTTTCTACTTTTAGCAAATTAGTTTTTTAATTTCGCTAAAGATTCAACAATATTTTTTGCTATAGTTTCTGGATCACTTGTAGCATCAAATGTTAATAATAAATTATATTTTTTGTAATAATCAAGCAATGGTGCTGTTTGATCATGATAAACTTTTTGACGTACTTTAATAGCTTCAATTGAGTCGTCTTTTCTTAAGATTAATTCAGCTCCACATTTATCACAATATTTGCCTTTAGTTGAAGGCATAAAATCAATATGGAAACTTGCTTTACATTTAGGACATTGTCTACGTCCATTTAATCTTTTTAAAATAACATCTTCAGGAGCACTTAATTCAAGAGCTACATATTTAAAGTTTGAAATACTATCTAAAAAGTTAACTTGGTCAATAGTACGTGGATAACCATCAAGCATAATAATCTTTTTAGCTTTGATTAAAGCTTGAATTTTATTTTTAACAATAGTATTTGTAATGTCGTCTGGTACATATTGTCCTGCTTCAACTATCTTTTTAACTTGTAAACCAAGTTCACTTTGTGAAGCTATTTCTTCACGAAAGATATTACCAGTTGAAACATGTACTAGTCCATATTTTTCAGCTATTATAGCAGCCACTGTACCTTTTCCTACACCAGGAGGCCCCATAAAAACTATATGTTGTTTAATTTTACTTATCATAATAAGCCATCTCCATTTTTGTATTTGTCATATTCTTTTGAAACGTCTTCAGATTTTTGATCATTTGTTGCAAAAGGATCATTTTCTTGATATTTGTTTTGGAAGTTAAGCACATCTGCAGCTTCTTTTGAAAGACGTTTTTGTTTTGCAAGATGTTGACTTTTACGGCGTGCCATGAATTGTTGAATAGTTTCAAGAGCAACTGATACTAAAATCATCATACCTGTACCACCAAAACTTACTATAGCTGGAATTTGTAATCATAAAATCATTATGTATTGGAATGAAGCTAAGAATACTAAATAGAAACCACTGAAGGTGCAAAGTCTTAAAACAACACCTATTAAATAATCTTCAGTTTCTTCTCCAGGTCTAATACCAGGAATAAATGTTGAGTTCTTTGCAAAGTCTTCTGAAATCTTGTCAATTCTTGATTGTTGCAATCCAATTATGTAANTAAAGAAGAATGTAATTAGAATCAATAATGACAAGCCTAGTGGTTGAGTGAATTGCATATTGGCTTCAATTCATAATTTGCCAGAATTACCTTTTGGAAGTATTCTAGATATCATAATTGGGAAGCTGACTAGCATTGAGGCGAAGATTATTGGCATAATTCCACCGGGATTTAATTTAATTGGCAATTTACCCATTTCATTAATATTTCTACTACGTCCAGCACCAATTTGTTGAATAGGTACATGTCTTTCAGCATTGTAAACTAAAGCAATAATAAATAACAATGCAAAGTAAACAAATAAGTAAGTTAAGAATTTTAATGCTCCAACAAAAATTGCTGCTGTATTTTCACTACCAACAAATAAGCTAAATGCTCCTTTAAATTGACTTGGTAATTGGAATGCAATACCAACAAAAATAATTAAGCTTGTACCATTTCCTAAACCTTTATCTGTAATTTGTTCAGCAATGAATAATGAGAACAATGATCCACCAATTAAGATTAAAGGCAATATTAAGTAGATTGTAACTTCTCGAGGGAGTTTTTGAATTGGAGTAAATCCAATAAATGGATTGTCACCTAAACTAAGTGATTTAGTAAGCAAGATAGCTTGAGGGAATGCAATAAACAAAGTCAAAATTCTTGTAATGATATTTAATTTTCTCCGACCTTGTGGTCCACTTTGACTAAGTTTTTGAATTGGAGGGAAAAGTTTGGTTTGTAAAAGAGACATTATCAAGCTGGCATTAATGAATGGACTAATTCCAAGTGCTGCAAGTGAGAAGTTTTTAAGACCTCCACCGCCAACAAGGTTTAATGTATTTAAGAATGGGTCATCATTAATATTTACATCAGATAATTTAACAAAAGGAGCTTTGATTGTTGTCATTAAAATATAGATTGATAACAACAATAATGTATAAAGCAATTTTTTAGTAATTTCTTTAGTACTTCAGAAAACTATTCATTTATTTTTGAAGTTATAAAAAAATTTTGAAATCTTAAATCCTACATTTGAGAAAAATTTCTTCATATATTCAACCCTCACTTTCATTGCAAAAACCAATTCAACTTTGTCTCTTATAAAATACTTTATTATTATATAATAAACTACCAAATTTTATAGTAAAAGTTTAATAAAAAAACTTATATTTTTTTAGCCTTAAAATAAATAAAAAATATTTTAGTTTTTTAGTATTTTAAAAAAATAAAAAAATTTCACCAAATCCTTTGTTTTATAGGCTTTTTGGTAAAAAAATGAATAAAAAAATGTCACACTCGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAACTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAAGTCTCACAGTCAAGCACACTTCTACCTTTGCGCTCTACATACGGTTTCTGACCGTACTGAGTGTACCTTTGAACGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCGTCACGCACTGTCCACCCACCTGATGATGATGGCATGTTAGAAACTCAATATACCAAGGGTGGTATTTCAAGGATGACTCCACGAGAACTAGCGTCCTCGCTTCAAAGTCTCCCACCTATCCTACACATGTTAGACCAAGTTCCAATACGAAACTGTAGTAAAGCTCCATGGGGTCTTTTCGTCTTGATGCGGGTACCCAGCGTTTTCACTGGGACCATAATTTCACCGAGTCTAGTGTTGAGACAGTTGAGAGATCATTGCGCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCATTTCAACGCTTCGCAAATGCTAACGCATCCACTTAACCTTCGGGCACTGGGCAGGCTTCACCCCCTATACATCACCTTGCGGTTTAGCAGAGAGCTGTGTTTTTGATAAACAGTTGCCCCTCATAATTTACTGTGGCCTAAGTGTTACCTTAGGCGCCCCTTCTTGCGAACTTACGGGGTCATTTTGCAGAGTTCCTTAACACTAGTTTTCTCGCTCGCCTTAGAATACTCATCTCGGGGACGTGTGTCCGTTCTCGGTACAGGTTTCCATAATATTAAGTTTAGAAGCTTTTCTTGGAAGTGTGGAATCATCTAATTCGGTTTGACCCTATGCATCACGCCTCCCGGTTATAGACTGCGGATTTGCCTACAGTCACCAGTGAACGCTTACCCCACAATCCAGTAAGTGGTAAGATTATCCTCCTCCGTCACTCCATCACTATTATAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCGTCTTAGGTCCTGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCGTAGATTCTCACTACGAATCGTTACTCATACCGGCATTCTCACTTCCTAGCGCTCCACCAGTCCTCACGGTCTGACTTCATTGCCCTAGGAACGCTCCTCTAACGTAAAATAATTACCCGCGGCTTCGGTATCGTGTTTTAGTCCCGTTAAATTGTTGGCGCAAGGTCTCTTGACTAGTGAGCTATTACGCACTCTTTAAAAGGTGGCTGCTTCTGAGCCAACTTCCTAGTTGTTTATGAAACCTCACAACCTTTCTCACTTAACACGATTTTGGGACCTTAGCCGGCGATCTGGGTTGTTGCCCTCGCGAGCCGGGACGTTAGCACCCCGGTTCCGACTGCATAGCAATACATAGCGGTATTCGGAGTTTGATTATAGTCAGTACCCCTAGGCGAGGCCATTCCATATTCAGTGCTCTACCACCACTACTTAACACTACACGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCACTATTCACAAGTCATCCGGGCACTTTTCAGCGTACTACGGTTCGGCCCTCCGCTTGGGGTTAGCCAAGTTTCAGCCTGCTCATGAATAGATCACATGGTTTCGGGTATATGACAACATACTAAGACGCCCTATTAAGACTCGATTTCTCTACGGCTCCGCTTTTTTCTGCTTAACCTCGCATGCTGTCATAACTCGCCGGTCCATACTGCAAGATGTACGCCATCACCCATAAACGGGCTCTGACTAATTGTAAGTAAGTGGTTTCAGAATCTATTTCACTCCCCTCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGGGTTCCACGTGCCCCGCCCTACTCAGGATACGATCAGAAGACTTAACAATTTCACATACGGGGGTATCACCCTCTATGCCGCTTCTTCCCAAAAGCTTCTGCTATCATTAAGTTTTGTAACTTCATGTAGATCGTCCTACAACCCCCATTGCTGGGTTTGGGCTCTTTCTCGTTCGCTCGCCGCTACTAAAGAAATCATTGTTTATTTTCTCTTCCTCTTGCTACTAAGATGTTTCAGTTCACAAGGTGTCTCACTCAAGTTCCTATGAATTCAGAACAAGGCAACTAGGCATTACCCTAGTTAGGTTTCCCCATTCGGAAATCCCCGTTTCATAGCATATTTCCGGCTCCACGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTTCAGACCCAAGGCATCCACCACAAACTCTTCCTTATTTAAAAGTATTTTCCTATTGTTATAGTTTGTTATGATGTGTATTTTAAGACATTTCAATGAATCATAATTAATATGATTTTCTCGATGAAATCAAAACAAATTAACCATTTAATTTAATTTTGATGTCGTTGTAAATATCAAAAAATTTACTATTCAGTTTTCAAAGAACAAATGAGAGAAAAAATATTGTTCTCTCAAAACTAGATATAAAGCTTTAGACCCATAAAAAAGCCACATAACAAAAAAATATATAAATAAAATAGGTCTAGTGAAATAAGGTTAATAGTACTTTAAAAAGTACTCTTTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACCCCAGTCACCAGTCCTGCCTTAGGCAGTTTGTTTATAAACCGACTTCGGGCATTACCAGCTCCCATGGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGTAGCGTAGCTGATCTACGATTACTAGCGATTCCGACTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGACCGGTTTTTTGAGGTTTGCTCCATGTCACCACTTCGCTTCTCTTTGTACCGGCCATTGTAGCACGTGTGTTGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCGATTACTCGGGCAGTCTCCTTAGAGTCCTCAACTGAATGGTAGTAACTAAGGATAAGGGTTGCGCTCGTTGCAGGACTTAACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCATTCTGTTAACCTCCACTATGTCTCCATAGCTTTGCAGAAGATGTCAAGAGTGGGTAAGGTTCTACGCGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGATCCCCGTCAATTCCTTTAAGTTTTATTCTTGCGAACGTACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGCCGATGAGTTACCCATCAGCTAATGATCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGTATATGTCCAGTTAGCTGCCTTCGCCATCTTGGTGTTCTTCCTTATATCTACGCATTTCACCGCTTCACAAGGAATTCCGCTAACCTCTACACAACTCTAGCCTGCCAGTATCCAACGCGTTTTGGGGTTGAGCCCCAAAATTTAACGCCAGACTTAACAAACAACCTACAGACGCTTTACGCCCAATAATTCCGGATAACGCTTGCAACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGTTGCTTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTTTGTCGCATTATTTTGTAAATGCTTTAATATGATAACACATAAATTAATATTTGCAAAACATTTTTTTGAAAATTTTTTTATTTTTTTACAAAGCATTTTAGAAATTATTTTTTTATGCTAATTTAACTTTTTTAAATTATGAAAAAATAAAAAAAGTGCCTTATTTTTAAAGCACATCTCTTATATATAAATAAAGAAAGGAATTTTTTAGAATTTGATTATTTCAATATTTTTTTCTAAGCATTCTTTTTCTAAGTCATTAATCATGTTTAATTCTTCTTCTAATTTAAGAGCTTCTTGAACATTAGGTTTAGTTACTGGTTCTTTAGGAGCAAAAAGCTCACAGGTTTCATTACATTTAGTAATTGAAAGATCATAGGTTTGAATTTTTTTAGCTAAATTGATTGTTTCAATTTTGTCATTAGTTAATAGTGGCCGCAAAATTGGCATGTTAATATCATGACTAATTGTATAAAGTGATTCTAAAGTTTGTGAAGCTACTTGACCTAAGTTTTCGCCATTAGAAATGGCTAGATTTTTTTTACTTTGAGCTAATTTAGCAGCAATACGATAAAAGCTCCGACGCATAAGAGTAATTTTATAAGCTTGATTTGAAACTAAAGCTATGTAGTTCATTAAAGTAGAATAGTTAGCTAAATAAAAAGTGCTTGTGTTTTGATATTTAGTTAATATTTTGATTAATTCTTTCATTTTATTTACTGTTGCTTGATCAGTTTGAGGAGGTGTAATAAAAGATAAGAAATTAACTTTAAGACCTCTTTTCATAAGTTGAAAAGCAGCAACAGGACTATCAATTCCACCACTCATTAAGTGCAATACTTGCCCACTAACACCAACGGGGAGGCCGCCTAAAGCTTGGACAAAATCACTAAAAACATAAGTAAATTTGCCTCTAACTTCAATATAAACAATAAAGTCTGGATTATTAACATCAACTTTTAAATTTTGGTTGTTTTCTAATAAAAAACCGCCAACTATATTGTTAATTTGACTTGATTGGTATTGAAAACTTTTATTGTTTCTACGAGCAGCAACTTTAAAAGTTCCATTTTTATTTTTTGCTAAATTTAAAGCTTGCTTTTTAATTTCTTCAATATCATTAGCACAAATAATAACAGGTGAGTAAGAAGTAATACCAAAAATGTATTGCAATGCATCTAAATTTTCTTGACTATAAGGCAAAAACATACGGTCAAATTCAACCTCTAATTTATCTTTAGTTTTATGACGAATATTTGAAGCTAAAGTATCAATAAAATTAACTCTATTTTTGCCTTTTAGAGTCAATTCACCATATCTAATTAAAATCTTATTGTACATATTTTTTTCCTTTTTTCATGCAGGTTGCTAGTAGTGAGAAAGCTTCATCAAATTTCTTAGCTCGCATGTGTTTATTGACTGAAATTAAGAGTTCATTAAAGTCTGGATTATTAAATCGATAATGCATACTTTTTAACATGAGTTCTTCTGTCATTTTCTTATATAAATAAGCCTTAAACGTCATTTTGAATAGCTTTGTAAAGATGTTGTAAAAGTGGTTTCAATTTTTAGATTTGAAGTTAATTTTTTTACTTTGTTCAAAGTCTTTTTTGACTTTTAATAAAGAAACAATTAAAAGCTTAATATCGTTTTCTAATTCTAAACTTTGTTCTAATACTGTGCTTTGAACAAATTGAATTTTAAGGCTTCAAGTTTCCAAAATTTTTAAGTAGTAAGCATTGTAATTTTTTTGATAAGTATCGAGGTTAAAAATCTCAATTTTATCATTGCATTTTTTAATTAATTCCATTATGCCATGAAGTAGTGTTTGAATCTCCATTAAACTAACATTGCCATTAGCTTTATTAGCTTTATTAATGCTATCATATTGGTTTCTAAGTTGTAACAATTCTTGTGAAATTTTGGTTACAAAATTGCTGTCAAAATCATCTAAATCAATTGAAATATAGCTTAATTTTTGATCAAGCTCATTAATCATTTTGCCTATGTTTTCTAATTCATTATTTATGAAATCTTGAAACTCAGCATTAAGAGCAGTTTTCTTGTTTAAAGTATAATAATCAGCATAGATTTTTTTGATTCTTTCTTGCACATCTTGATAAGGTAATTTTAATCATTCAGTTGCTAAGTTTTCTCTTTCGTCAATTAATTGTTTGATAGCTAATTTGTCTTTTGTTTCAAAAGAGAGCTTGCTTAAGATTTCAGGTAAGTGGTTAAAAAGACTTCATTCGATATTAACAAAGTGGTCCACTTTGCGAGCAAAAATATTGATTCTTTTTTCATGTTGATTTAAAGCATTTGAAATACTATCAATTTTATGAATAAGTTTATTTTCTTCTCATTCAATTGTAGTGTTACTTAATTGTTTTAATTCATTAGTTAAATAATCATAACTATGATTTAAGCAATATTTATTTTTTTCTAAATGTTCTTCAATTTTTCTTAAGATTCTAAATGATTCATTGGTTACTATTTCAATAACATTTCAATGTTTATTTAAGTTCTTAGTTTTTTCATCAAATTGTTTATGCATTGAAATGCATTCTTCATATTTAGGCAAAATTTCTAAGTAAAGTTTTTTAGCTTCTTTAAGTTTATAAGCTTTAGCCGCTTTTCTTAATGCATTATATTTATCATTTAAATCAACAATTATTTTTTTTAATTTGTTGTCTAAACTTTTTAATTGATTTAAAGCAGCTTTATATGTTTCTTGAGTATTTGATAAAGTAATAAAACGTTGAATTGTTGCGTTACGTCCTGTTAGATCATTTTTAATATTTTTAGTTGAATCATCTAAAAATTTAATGTATTTATTAAGATTAGTTTTTCTAATATAAAGGAAAATAAAAATGCCCAAAAGACCTAAACAGGCCAAAATAACTATTGGTACAATAATTTTAATTACTTGCATTTTTCTCCTTAATTAATAATTATATTTTATATTAATATCATTTTTATTTATCAAAGTAGCATTAAAGAATAATAAAAAAATGGGATTGCCCATTTAATTATTATTAAGCATTACGCATATCTAAACTTTTGTAACCTTCAACTTTTTTACCTTTATAAAATCCACAAGATTCACATACACGGTGTTGTTGAATTAATTGAGAACAGTTTTTGCATGCAACAAGATTTTGAACAGGTAAAGCATCATGGGTACGTCTTTTGTGTTTACGTTGTTTAGATGTCTTACGTTTTGGAACTATAGCCACGATTCCTCCTTATACAATTTTTATATTAGCTAATAAATGTATTCTAATTATATGTTAAAAAATTAAAATGTATAGCAATTTATTAAAAATTTTTTTTGTAGTGTAACTAACATTTTATTGCTTTTTAAAGCAATTATTTTTTTGGCCTATTTTACAGTACTTTTAGTTAAAAGTAATATCTTGAAAATAAGTAAAACCATCTAGATTTATTGGTTTTGTGTAGAAGTATTGAACTATTGATTTTTGGTATTCGTTAGTAAAAAGGAAATTATCTTCAGTTACTCTGATTCCTAAAAATGAGTCAATACTTTTTAAAATATTGAATTGTTGATCAAAATTTAAAGCTTGAATTTTCTCTTTAAATCTAGCTTTGAATTCTTTCAATTTCAAATTATTTTTTTGCAAAATTTGATCTAAAAGTGAACTGTTTGCTTTAATTTTTTGCAACTCATAATTTTTAAAGTAATCAACTTTATTAATTGTTTCTTCTATTGTTGAAATAATTTTTTTGATATTTTTATCATAGTTAATTGATTCTAAATTGACTTTTGAATATAAGAATAAATTTAAAAAGAATTGGTTATTTGCTGTTATTAAATTAATTAAATAGTTACTAAATGAATTATCAACTGCACTAAAGTTTTGATATTTAGAAAAGTAATTATTTGCTTCTTTTGAAGCATATTCAAAATTAACATTTAAACGATTGTCTAGGTTGTTGTACAAGAAGGCTATTCTTTTATATAAACTGTCAATAAATGAACTCTTTTCAGCAAAAATAGGTAATGTAAATTGAATTTTTTGATTTTTATAATCTCCATTTTTGCTATAAAAATCATCTAAAATTTGCTTCATTTCATTTTGATAAATTTGATAATTTGCACTCTTTAGTTGTTCATTAATATCCAAAATATTTTTGCTATTTATTGAATGTTTAATTAAGCCATCAGATCCATTAAAATCAATTATTTTATTATCAAATTTATTAGTTTTAGAATCAAAATGATAATCATAAACTCGATTAATGTCATCTAAAAAGTTAACATTTTGGTTGTCTAAAATACCACTATTATTGCTGCTGTCATAGTTTAAACTTTGTGAAGCAAATGAATTTCAGTAAGTATTATGATTCAAATTTTTAATAAATGAATATTGATTAATACAGTTATTTAAAATATTTCTGAACACAATTTGATGTGGTTCATAATATTTTTTATTTACTAAATTTTGATCTTTTAATTCTTTTAATTTCTGATTATAAAATAGTAAGCTAAAAGCTTCATTAAATTTGAAATCTTTGTTATTTTTAGCATATGGATTAACATTGTAAAAATAGCTTATAACATTACTATTATTTGGTTTAAATAATGTATAAGTAACACCAAAAAGATTAGAGAAATTATCTATTTCATCTTTTTGCAATTGGTTAAAAATATCATAAGAAGCTTCACTTAAAAGATTTTGTTTAAATGCATTATAACTTTGTAATCTAAAGGTAGGCTCATCGCTTTGAATTGGATTATATTTTATAGTCACTTTATTAAGAATATTTTGCTTTTCATTTTGGTTAAAACTATCTAAAGCAGAGTTATTATTTTTAATAAAAATTTGCTCATCAACAGTGTTCTTATTTAAAACATAATAACCTATAAAACAGATGTTTTGGTAATTTTTGCCATAACTATTTTTATTATTTTTATAGTAGTCTGTTGATATTGGATTGAAGATCAAATTATTAGTAATTTCTTGAGTTGTAAATAGTTTAATAAAGTTATTATTTTTGCTTTTTTGAGCATCGTTTAATTCAAAAATTAACTTGTTATTTTGGATGCTATTTTGGCCTAAATTTTTCAAAGAATAGTTGTCTAAAAGAGCTGAAAATTGTTCACGATTTTGTTTCAAATTAAAGCTAGTTAAATAGTCTTGGGCTTGTAATTTATGATTTGTTTTTTTGCCTTTATAATCTGAATAATAAACATCGTCTTTTAATTCAAATTCAATTTTAGTTGCACTATTTAAAGCTTCAAAAAATTCTTTAGAATTTATATTATTATTTTCATCAGAATAAATTTGTTTAATACCGCTTAATGCTCCATCTTCATTAACTTTAGTACTACTTTGAAGAGCTTGATCAACTTTTTCTTTAACATCATCACTATTAAAAATTTTTTGACTATTATCCTTCAAAGTCACTGTTATTGATTTAGCTAAATAGAATTCTAAAAATCTAGTTGTAGTTTTATAAAAGAATTTGTTTAAGTGATCAAATTTACTTTTGCCTAAACTTTTTCATCTAATTAAAGGTGCAGTTAATAAACTCTCAAAATCATTATCAATTCTTTTAACTTTGTTAATAATAAAATTGTCTTTAATTTTGTTTGGTTCATTATATTTTTTAATATAACTGTCTTTATGATTAACTTGTCTAATATTGTTGCATGAAAGAGTAGCAACACTAGAAGTAAGTGTTGCACTAATTAAAAATAAGCTGCCATATTTTTTAATTTTTCTTTTCATACTATCCTTTACTTTTCTAATTTAGGTACACTCGCAATAAAGAAATTTATACCTAAGAATTTTATTAATATTACATAAAAACTTGCTAATATTGAAACAAAAATTGTATATTCAACATTTTTTAGATTGTCAACTATATCTTTAAAAACGTTTCCGATATTTAAGCTTTGATCAATTTTATAAATATTAAAGAAAGCTAATGAAGCTAAAGCTAACATATTAAGTGATAATGAATCAGAAACTAAAGCAATATTTCTTCACAAGCAGTGAATGAAAATAATTTTGTAAATTATTCTTCAATTACTCAAGCCATTAGCTTTGTAAGCTAAAATAAATTCACTGTTTTTGATTTCAAGCCCTATTGAATAACTTGAATAAAAGAAGCTAAGGGAACCAATAAAACCTAAAATTAATATAGCATTAAGATGGTTATATGAACTTATTTTAAAAATCAAGAAAACTACAATAAGGAAAGGCACAATATTAATAGTACTAATTAAATAGTAACTAAATTTAGCACTGCTTTTATTTGAATAATAAGCCACTATTGAACCTAAAAAGGTCCCTAAAAATAATTGGATTAAAGCAGCCACAAATGTTACTAAAATACTTATGCCAAATGAATGAATAAAGAAACTAAAGCGGTCTATACCTTGAGAATTGGTACCATATAAAAAGATATAGTTAGTGCCTTTTAAGGCATAAAGCAATTGATATGGATTGTAAGTTAAATTAACTAAGTCAAAATCAGGTGTTTCATTTATGACTATTTTTAAATTAGGATGATCTTTTTGAGTTTGCAAAATAAATTGATATAAGGGGTCACTTTGTGCAAACTTTTTAGTAATACTTGCATTTAAGTAGTTAGGCAAATTATAAGCAAGTTGTGAATCGATGACACTAGTTTTTGGCGAATAAGGGCTAAAAATAGCACTTAAAGTCAAAAACAGTAAGAAGATTCCAATAATTGAAACTAAAGCAATGTTGATCTTTTTGCTAAAGAAACGAATTAAGTATTGTTTAAATTCACTTTTGTTATTGCTGATATTTACTTGGAAGTGTTTATGGTGAGTTAAAGTGAAAAGACTTTTTTCAAATTCAATATGACTTTGGCCTGCTTTTTCGTGAGAATGGTGGTGCTCATGATTAAATGCCATTATGCGCCTCCTTTCTGAATCTAGCTTTTTCTCTATTTTCTTTGAAGTTAAAATTGAAATTAAAATTATAACCATTTTCAACTTGCAATAAATCATAAGTTAATTCACAAATAAAAGCTAATAAGAATAAAAGCAAAGCTTTAAAAAATACTAAAGCCATAATAACATTAATTTCTTTTTGATCAAACATGCTAATTAAAATAACACTTTGACCTGGTATTTGAAAAATACGTTCGATAATTAAGCTAAAACTTATAGTTAAAACTAAAATTGGCACTATTTGGTTAACTAATGAAATAAATAAATTTTTAATAACAGCTTTATTAAATATTTGTAAAGTACTTAAGCCATTTGCTTTTAAAGTGATTACATAATCTTTTTTTAGAATTTGAACAAGATTATTTTTGGTAATGGTTGCAAAGAATGCAATGATTCCTAAAGATAAAAGTGAAATAGGAAGTATTAAAGAAAGCAATGTATAACCAAAACCAAAAGCTGATGGCTCTATAAAATTAACTGGTAAGTCATTGACTTCTGAAATAATTAGCATTATTGGTGCAAAGATAAAAGTAGGAATAGCAGCTAAAATAAAGACAATAAATGAGAGGACTAAATCAGTCATTTTATTGTTTTTATAGCCTAATCATATTCCGACAAAAACACCTATAAAAAAGCTAAATACAAAAGAAATGCTTACAAAAAGTAAACTAAATTTATAATAATGGAAGAATAATTCTAACGATGAATTGAAACCACTATTATTAGTTGAAAATGGTTTACCAAAACCACTAAAAATAGAACCTAAGAAAGTGAAATAATTTTTGATAATTGGTTCTCTGTTTATTTGTGAGTTATTAATAAATAAAGCTATAACAAAATAGCCAAGGGTAACTGTTATAAAAATACCTAAGAATGTAAGTAGAATTTTTTTGAAAAAGTATTTGCTAAAATTCATAAAAATTCATCTTTCTACATAAAAAAACCGCTTAATGGCGGTTTCTTATTACATTAAATAATTTCATTTGATTTAAAAGCATCATAAATAGCTTTGTATGCTTCTTCTTCATCTGCTCCATTGATTTTAATAGTAATTACGTCGCCTTTTTTAACACCTAAAGACATAACATTCATAATTGATTTAAGATTTCCAACTTTTCCTGTCTTGTCTAAAACAATTTGCACATCACTCTTGAATTTTGAAGCAATTGTTGAAACAACACTAGCAGGTCTTGCGTGAAGACCAATTGGATCTATAATTGAAGCTGTAAATTCTTTCATGTTTATCTCCTTATTAATTTAATATTTCAAAATATATCTTCTTAAATATCTAAAGTTGCCAATAAATATTATTAATTTTATATACTAATTCTTATATATACTTATAATATTACTACAAATGTCATTTTTAAAATATAAATTTTAAAGAATTTTTTAGTGGTGTTTTTAACTTATAAACCTTTAAAACAGTTTGTTTGGTAAATTATTCTGCTTTTTCAACTTTGAAAAACTTTTCGTTTAAGGTTTGAATATTTTTTCCAATGTTTTCAAAATCGATATTACTACTAATTAATGCTTTTGTTATGTTTGTGATTTTATTGAAAGGTAAATCAACAGCAATACCCGTTTCTCGACATTGATATTCTTTGTTTGATGAAACTAGTTTTGCTTTTTCTAAGGCTTTAATAGTTTCATTAATTTGTTCAGTTTTAATAAATACTTCAAAGTTTGAATTTTTTAAAATTTCTAAGTCACCAGAATATAAAACTTTACCTTTATCTAAAACAGTTAGACTATCAATATATTGTTCTAGCTCACTTAAAATATGAGAAGAAACAAAAATGGTTTTGCCTTGTTTGTGAAGTTTGCTTAAGTTAAAATAAAGTTCATTTCTAACTTTTGGATCTAAGTTTGCTGCAGGCTCATCTAAGATCAATATTTCTGGATTATGAATTAAAGCTTGGACTAAAATAACTTTCTTTTGTTGACCTGAAGATAAATTTTTACCTATCTTTTTTCATATTTTTTTGTTAACATCTAACTCGATTAATAATTCTTCAATTCTTTTTTTAGCTTTGTCATTTTTAAGTCCATTTAATTCAGCTAAAGCAACTAAGTAATCATAAATATTCAGACTTTTAGGAAATGTTGCTACTTCAGGAACATAACCAATTTTGATTCTACTTTTTTTGTTTTTAAAGTTAATTCCATCTATTAAAATTTCACCTTGGACTTCTGGATAAAAATACAATAATGAACGGAAACTTGTAGTTTTACCTGCTCCATTTGCTCCAATAAATCCATGAAATTCACCTTTTTTGATTGAAAATGTCACATTGTCTAAAGCTTTAAAATTTTTAAAACTCATTGTAACATTTTTAAATTCAATTGCGTTTTGTGACATTTTTTCCTCCTATTTTAAAAAGTTAGTTTTTTTGTAAATAAATCATCCGCCAATAGCTAAAGGTGTAGCCATTAAAAAGTAAATAATTATTTGCATGTAAGATGGAAATATATTAACTTTTTGTACTTTGTAAAATTTCAAATCAATGTTGTTTTCTTTTAATAAATCTTTATTAATTAGCATGCTGCCAAAAATTTCTTCAAGTCTTGAATTAAAGAATTCTTTATTTTGATTTATTTTTAAAGTTTCGCGATAATCATTCATAATAACTTTTGACATTGCATCAACTATAGAATATAAATTATTTTTTACCAAGATTGTAAAGAAAAGTTTTTTTGCAATAGAGTTATAGTCAAAGCTTAAGTAACGAGTTTTTTTAAATAATTCTTCTTTTTTGTCAAAAGTTAAATTATCTTCAACAAAATTTCTGAATTCTCTTTCTTCATTTTTTGCTTTTGAAGGATCTTCATTAGCTGAGAGAAAAATGGTACTTCTATATATTAATGGCACCATTTCATTGTAAAGTCTTTGAGTTGCAAAATTAATTAATGTTTCATATGTTTCAAGAGTTTTGGTTGAGTCTTTACCATCAAAATTTGCTTTAGCATTGACAATTTCAGTTTTGAAATCGGTTTTTAAATTTTCAATTACATTAACAAGACCATTAAATAATTTTTGACTAAAAACATCTGGATTTGCTTTTAAAATTTGGGCTTCTTTTTCAAAACGATAAACTCTATCAGTATATTCATCATTTCATTTTATAATGTGTTTTTTGCGAGCCATATAAGTAATTTTTTGTTCTAAGGTTTCTTGTTTTTGATATGGATTAAGTCGATCTGTTGTGCTTAAATCATAATCAATAATATAAATCTTTTGATTTCCATTTGTGACATAATCATAATTATTCAAAACAGATTCCATATCTCTTTTATAAAAATATTGGTTATGAACTCCTTCACTCATAATTGGAATAAAGCGGTTGCTCTTTGGATCATCTAAAATAAAATCATTATAAAAGTTCATTGATCAAAAGTTCATATTTAAACCATTAGATAAATTGACAACTGAAAAATTATGTTTAAATAAATTTGAGCTTGAAGTTACATAATCATTAATAACAAATTTTTTGTTTATTTCATTTTCTGCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAAGTTAAGCCTTCATTTTGAGGCATCTTAAAAATATAAGAACCCGTAATAATTGCGGCCAATATAATTAAAATAGGTGACATGCTTCCATAATAAATAACTATATCTCGAGCTGAATTTGTGTGAGATACAATAAGAATAATAAGGGTACCTATTAAAAGTAAAAACGGTACTAGGAAAAAGTTAATAATTAATATTGACTTGTCTTTGTATGCTGTTTTAAAGTTTAACTTTAATAATTTTCCCATTTTTTCCTTTCATATTTGTATGCTACTATTTTACTAAATTATTAATAAAATAGTCTAATTTGGAAAAAATAACCACAAATTTGAATAAAAAAATGTAGCTTTCTTGCTACACTAATTATTAGAATGAAAATTACTACTATTTTCTTAAGTTTAATTCAGCTAAAACTTTGTTGTATGTTTCTAAGTCATTTGACTTAAGGTGTTGTAATAAAACACGACGTTTTGAAATTTTTGCTAAGAAACCACGACGTGAGTGGTTGTCTTTAGGATTTGCTGCAAAGTGTTTTTTCAAATCTTCAATTTCTGCTGTTAAAATTGCGATTTGAACAAATGTGTTTCCTGTGTCTTTTTTATTAGCACCATACTTAGCAACTAATTTAGCTTTTTCTTCTTTTGTAATCATATATCGTCTCCTTATATATGTTCTAACCTAGCTAAAATCTAATATCTTAACCAAGTATAAAATTTGAATGCATTTTTTAATGCCTATAAATAATAGCACATTAATAATAAAAGCAAAATAAAAACATTTTGTGACCTAATCGTATTATTTTCAAAAGTTAGATTTTTAAAACCTGGATGTTTCACTTTTGGAATTAACAATAATTAGTAAAAAATATTAAAAAATTTTTGAAACACACGGCAAAGTTGTTATATAATAGAAATCACTATATTTCATTTATAGTATTTAGGACATTTATTAAATAAGAGAAAGGAAACAAAAATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAAGCCTACTAATATAGAAGGAGAAAATTATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGATTAGTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGATAATATAAGATATTATGGCAAGACAATATAAATTAGAAGATTACCGTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGAATGGCCCAAGAACAAGAACGTGGTATTACTATTACTTCAGCTGCTACAACAGCATTCTGAAAAGGAAAAAGAATTAACATTATCGACACCCCAGGACACGTTGACTTTACAGTTGAAGTTGAACGTTCACTTCGTGTATTAGACGGTGCTGTTACCGTTTTAGATGCTCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCTACAAACTACAAAGTTCCTCGTTTAGTTTATGTAAACAAAATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCAGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGAATCAAATTTGAACCAAACCATGACAAAGGTTTTGAATTCGTAGACAAAATTGTTGGTGGTAAAATTCCTAAAGAATACATTAAACCAATTCAAAAAGGCCTTGAAGACAAAATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAATAATACAAAAAATGAACTCATAAACAAAAAACCGAGTTCATTTTTTTATATCATAGATATAAGGAAATTAGAATAATCTAAAATGTATATTTTTCCAGAATTATGAATAATTTGATTAATAAAAGGAGTAAGTTATGACAGATAAACTTTTTGAAAAAAGCAATATTTGCCTAGACTTGTTGACCAAAAAATAAAAGATTCCTTAGAAGTTATTGGAGGAATATTTTTGGTTGGCCCTAAATATTGTGGCAAAACATGAACAGGAATTAATTTTACTAAAAGTCAAATAAACATACTAAAAAACAATTTAGACTATGTATTTGATTTAAACAATACCCTTCAAGGAAAAACACCTCGACTTATAGATGAGTGACAAATTAGACCAGAAATTTGAAATGCTGTAGTCAATGAAATTAGTGTTAGAAGAATATATTTTAACTGGATCAATACTAAGTAACTGAGACAAGATACAACACTCTAACGTTGATCGTATCGGAATGTTAAATATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGAAAAGATGGATGACCTTCTTTAATTGACAATAACTCAAATGCTCTAGTTCAAATTTGATTTGATTACTATATTAACAATATAATGAATCTTGATGAATAATATTTTAAAGCTAGAAAAATAGACTTTGACAAATACAATTTATTTCTTAAATCACTAGCAAGAAATGAAGGTCAAACAATCAAATTAAGTGCTATTGCAAATTATATAAATAATGTTGCTAAAATTGATACAAGAACAATTGATGATTATTTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGAAATATTTTACATTATAGAGATAAATGGTCAAATGAGGTAGACTGTATTTTACAACTTAAAAATGGAGACTTTGCAGCCATTAAAATTAAATTAAGTAATAGTTATGAAGAGCAAACAGCTACAGGCTTACTTAGGTTTAAAAAAGAAATGGAAGAAGAACAAAAATTAGCTAAAATCAATAGGGAAAAAATGTACAAAAAAGTACCTAAATGATTAATAATAATTAGTGGGACAGCTCAAAAGGCATATCAAAGAGAAGATAAAATTTTTGTAATTCCATATAAAATGCTTTGTGAATAATTTTGCTTTATAAGCCTATAAAACAGCCAAAAATAAAAAAGACCATTATCTAAAAATGATCTTTATTCCTTTGGCTTTTGATTCATTAGAAACTATGTCCATAATTTCTTTTTTAACTCTTGGATCTAGATTAATTTTTTCACTTTCAAAATAATCTTTGAACATTGTACGATCATTATGAAAAAGAAAAGTCATATAATAAATTTCAGCTGTTACTTCAGGGAATTTTATTAACTTTTTCAAGATTAGAAATGATTCAACTGTAATACTTAAATAAACAATTACAACTATCATACAAAATGCTGCTGCAGTCGCCGCTAAATCTTTAGTTTGCTTCATTATATTATTGTCATATTTATACTCTAAACCTTTGTAAATTGGCATAATAAAGATGCACAAATAAAAGATAAAAGCCATTAAACATGAAGCAATAATGTTATATTTTGAAACGCCTTTACGATCAAATTTAATTCAAAAATAACAAGCTAAAATAAGCGTAATTAATGAAATTATTGAACTAGTTGCAATATAAACAAAAAAATTATATCAATAATTTAAACCACTTAAATAGTAATCATAATTCTTTAATGCTAAACTCAAAAAAGTTACAGTAATAGTAAAAGCTACTAATAAAGAAATAAAAGCAAAAAGAGAACTTCAAGACAAATCTCTTCTTAATTTAACTTCTTCTGTGTGATATTTTTGAGCTTCTTGATTAAAAGTTTCAAGCATTTTGTATACCTCTTAACTAATTTTATAAAAAATAATTTTTTTTAATTGAATTTAATTTATTTTAGTGTATTCTTATATTAATTAGAAGCCAAAAATAGGCTTCAAAATAATAAATATAAAGCATAAAGAGTTGCTGAGCTAGCTGGCTTTGACGCATGCCAACCTGCATTTGTAAGGTGGACAGTGAGGGTTTATTAACTAAACTAACATGTTTAATTATTGAGCTAATAACCTCTAACTTTTTATGCAACAAAAGTGGAGGTTTTTTATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGACCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGAGAAAAATTAGACAAAGTTGAAGCTATCAAAAAATTAATGAAATAAATTATTTCAAAATTAAAAAATAATGTAAAATAAATACACTTATATTTTTGATTTTAATACACTAAGGAGGAACATCTTATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAATAATCATAATAATAAGCAAGGAACAATTTTGTTCTTTGTTTTTTTATCCTTTTTTAGGAAGATATTATTAATTTTGAATTAAGTTTTTTAAACTTGGATGGTTCTCACTTTTATAATTTTTAGAATTGTGGTTCAAAATATAATACCTATTGATTTACTTGGAAATCTGAATATTGAAATTCAGGTACAAGATATTAAAACTAATGAAACATCTAAAGTAAGAAAATTTAATTTACTTTTTAAAAGTTTGCTAAATAAAAATAAAAGAGCCTTATAAAAAGGCTCAAATATTATTAATTAAGTATGAAATTATTTAACTTCAACTGTAGCACCAGCTTCAACAAGTTTAGCTTTGATAGCTTCTGCTTCATCTGGTTTGATGTTTTCTTTAACAACAGCTGGAAGGTTTGAAACTAATTTGTTTGCATCCATTAAAGCTAAGTTTAATAATTCTTTAACTGCTTTAACAATAGGTAATTTTTTACCATTGTCTGAAGTAATTGAAACTGTAACTACTGAAGGACCTTCAGCAGCTGCAGCTGCAGCAGGAGCAGCAGCAACAGCAACAGCTGATGGGTCAATTCCAAATTCTTCTTTCATTGCTTCAACTAATTCCATAACTTCTTTAATTGACATTTCTTTTAATGATGAAATGAATGATTCTTTTGTTAATTTTGCCATGATTAATTTCCTTTCGTAAAATTATTTGTTTTCTTCTTTTTCACTAAACATTTTTAGAGAAAGAGAGAGTTGTTGTAATGGTGACATAAGTGAACGAGCAAGAATTGTAAGAGCTTCTTCATAGTTTGGTAATGAAGCAACTTCTTGAACACCTTTAGCATCGATAACTTTGCCTTCAAAAATACCAGCTTTAGGAACCAAAAGTTTGTTTTCTTTAGAGAATTTAACTAAAACTTTTGCAGCAATTAGAGCATCGGCTGTACCAAAAGCAAAAATGTTTGGTCCTACTAAGTGTTGATCTAAATCATTAATGCCATGATTTTTAGCAGCTATTTTGAAAAGACGGTTTTTGTAAACTTTAATGTCAATACCTTCTTTTTTAGCTTCTTGACGTAATGAAGTCAATTCAGCAACACTTAATCCACGATATTCAGCTATAACAAGAGCTTGTGAAGATTCAAGTTTATTTTGAATTTCTTTAACTGTATCTTTTTTAACTAATCTAAAAGCAGATTCATGTCTTTTTTGCATGAGTATCCCCCTTTCATAATTTAATATGCAACACGGGACAATAATAATGTGATAGAAAAAAAGTAATTCTACACTCGGTAACGAATTAAGCTTTCGCTGTTACTGTCTTTATGTATTGCGTTTTTATTATATTAATAAATTTAAAAAATCAAAGCAATTTTTTTACTAATTTTAGCCAGTTGATTTTAATAGAAAAAATAAAAACAGCAAGTAAGCTGCTTTATTAATTTTTTTCATTATCAGTTAAGGTTTCATCAGTATTTAAAATGTCTGCTTTATTACCTAATTCAATATCTACACGAACATTTTTAAATCTGTTAGTTCCTAAGTCTATAACTTTAAAGTAAACATCATTGTATTCATATTTTGTATTTTTATAAAGTTTGTGGCCGAGTCTTTTTTCTAATCATTTATGAAGTGTTAATTCATTTTCTTCTTCTTCAAGATCTAATTCTATTTCTAGTTTTTTAATTAATTCTTTCATTGTTAAAGAAGCTGAGACATGGAAGTGTTCAAGACTAATTTCAAAAATGTCTTTTAATTCTTCATCATCAAATTCATCATAAATTTCGCCAACAATTTCTTCAATAATGTCTTCAATTGTAATAATTCCAATTATTTCACTTGAGTTGTTATTTGGTGTAACAAAAGCCATTTGAGCTCTTTGGTATCTCATTTTTTCTAGAGCCGAAGAAAGAGAAGCATTAGCACTAATAGTAGGCACAGTTTTAAAATAGTTTATAACTTTACCTTTTTGTAAAAAGAAGACATCTTTTAAGTGTAAAATGCCAATTAAATTTTCATCTTTTTTAACAGGGATACGGCTATAGTTTGTATCTTTAAAAATTTCAAATGCTTCTTGAATATTAGCATTAGCATCAATTATAGATACATCTTTTAATCTTATGTAGTGTTTTTTAACTTTGGTACTATCTAAGTCTAAAGCATTTTGAGCCATGATGCTTTCATTAGCCTCTAAAACGCCTTCGTTTTGCGCAATATCAATAATATTTTTAACATCATTTTCTGTATTTGTTATATAGATTTTTTTACCAATCTTGCTTATTGGATAGGTAATTGGTCAAAACAAGTAATATAAAATAATTAACAAGTAGCAGAAAACTTTAACTGTCGCTTCAGGTTTACTTTTGGCTATCAATTTAGGTGTAATTTCTCCAAATAAAACAATTATTGGAGTCATTACAGCAGTTGAAATTAAAACGTTATATGACTCGAATTTTTTACCTAAGTAAGTTGATAAGAGTCAGGATGTTAAGGCCGAAGCAGCAATATTAACTATATTGTTGCATATTAAAATAGTACTTAAAGTTTGGTTGAAAAATGTATGTTGTTTTTTGATTATTTTGTGGCCAAATTCTTTATTGCCAACCATTGTTTCAATTTTGCCAACATTTAAGGATGTATAAGCAGTTTCACTGGCACTAAAAATTCCGCTAGCTATTAACAAAATAACTAGTCCGAATATGCTTAATCCTAATTTCAGTTTCGGGTCCATGATTATTCCTCTTCTTCGTCATCATCACTTGAGACGGCAATTTGTTCATTTTCAAAATTAATGAAACGACCACATGACATTTTGAATCATAATGGAAAAATTCCTGTTGGTCCATTTCTGTGTTTTGCAATAATGATATCTATTTTTTCTCCAATATCTCCAAAGAGATCTTTGGTTTCTTGTGAGACTTGTTTTTTATAATAATTTTCACGATATAAGAACATAACAATATCGGCATCTTGTTCAATGTTACCTGATTCACGTAAGTCAGACATAATAGGTCTTTTGTCTTCACGTTTTTCAACTTCACGGGAAAGTTGGCTTAGTGCAATAATTGGAACATTTAATTCTAAGGCTAATGTTTTTAATGAACGGCTAATTTTAGCTATTTCATTTTGCCGACTTTCATTTCTTCTATTTGAGTCTGATGAAATCAATTGTAAGTAGTCAATAACAATTAAATCAATTGGACTAACTTTGTGTAAACGACGACATTTTCAAAGCAGTGTATTTAATTCATTATTTACTGATTCATCTATAAATAAGTTCATTTTGTCAATTACATCTTGTTTTAAACTGTTGATTAAAAGAAATTCATTATCTGTAATTGTTTGAGGTTGTTTTAGTTTTTGAAGTGGCACATTTGTGTTCAAGCTATAAATTCTACCCATAAGTTGGTTTGATGACATTTCAAGCGTAAAGAAGACAGCTCTTCTGTTTTTTCTTTTAGCAACATTTGAAGCAATATTAAGAGCAAAAGCAGTTTTACCCATTGCTGGACGAGCAGCAATAATAATTAATTCACCACCATGTAGACCTTGAGTAATTTGGTCTAAATTGTCGTAACCTGTTGCTAGTCCATTTAATTCATTTAGATCTCTTGAGTGGCGATTTTCTAAATCTTTAAAGTATTCATCGCTAACTTCTTTGGCTGTTAAAAAGTCTGCACTAACTTGACTACGATCAATGTCAAGTAATAGGTTTTGTAAATCTGAAATAACATCTTTTTCATTGATTGCTTTTTCAACATCAAGTCTTCTTTGAACTAAAGCTAAACGTGATTCAATTAAACGTAATTTTGTTAACTTAACTAATTCTTCTAAGTAAGTTTGAATATTAGATGAGAAACCAGCAGTATGATAAAGTTTAGATAAATATAAAGGTGTTATTTGAACAAAGTTTCTTTTTTTAGCAATAGTAAAAATGTTTTCTTCATTAATCGAAATGTTGTTTTCAAATAGTTCTTTAAAAACAGCAAAAAGTCTTTGATTTTCTAAAATCGCAAAGTCTTCTTCAAGTAAGTAAGGTACAATTTTAGGTGCGTTTTTGTTGTCTGTTAAGATTAAGCCTAAAAGAGACTCCTCATATCTAGAGTTAATATGACGAGATGTATTTATTGGTGCATTACTTGGATTATTATTTGGCATTAATTATCACTTCTACTTTCAATTTTGCAATGATATCTTTATATAAAATAACATCTATATCATGCAATCCATTAGATACTAAATGAACTTTTTGAACTGCGTATTTATCTAATTTATAGCCATTTTGAACTAAATCTTTAACAATATCTTTGGTTGATACAGCACCATGAGCAATTAAATTTCCATTATTGTCAATATGTGCATCTAAAGTATATTTTAAAGTTAATTTTTCAATTTGATCTTTAAGTTCAAGAGCTTCTGAACGTTTTTCCATTTCATTTTTAGTAAGCTCACTAAGTCTTTGTTCAAGCTCTTTTTTAGTCTTGTCATTATAGACAACTGCAAAACCTTTTGCAATTAAGAAGTTATTACCATAACCAGCTGAAACTTCAATAATTATATTGGCTTTACCTTTATCACAATCTTTAATCAATATTACTTTCATTTTTCACACTCACTATCGCTTGTTTAATATTATCTACAAATGTCAACAAGTCTTCTTTTGAAACTGCAGCTGCTGTCCCAAAGTGTCCACCTCCACCAACAGCTTCGCAAATAATTTGAACATTTGTATCAATGCCTCTGGCACTTAATTTATATGATTTTTTATCTTCACTTTTAGCTACAACAAAACTTGCTTTACGACCACTAATTCTAAGAATTTCTTCAGCTGCTATAGAAATAACATCATTCGAAAGTGGAATATCTTTGTAAGCTAAATAGAATCCTGGTTTTACTTCTTCTAAGTTTTTTAATACTTGATCAACTTTTTCATAAGTGGTTGCATCAATTTTTAATGAATTAGAACTTTCAGTTGAATCTGCGCCTCTATTTTTAAGTCAACTTGCTGCTTCAAATGTTCTTGAAGTAACATGTTTTTGAAATTGTAAGGTGTCAAGATAAATACCATTTAATAACATTTGAGCAATGTTCTTTTTAACTTTAACTTGCTTTTCTAAAAACATCATTAATTCAGTTACTATTTCACTAGCACTTGAAGCTGAAGGCTCAATGTGGCGATTAATTTTTGAACAAAAGTCAACTGAAGAATTTAATCTATGGTGGTCTAAAATAAAGATATTTGTGTTTTTAGCTTTTTCAACACATTTTGGATTATCAGTCCTATTTGGAGCTGAGTTATCAACTAAAAATACAAGTGTATTTTCATCAGTCAATGTGTTAGCTTGATGAGCTTTAATGAAAATTGAATTATCTTCACCAAAGTATTTAGCAATTGCTTTTTTAGTTGTAACATCTTGAGTCATTGTGCAAATGTAAGCTTCTTTATCAAATGTTTTAGCTATAGCATAAACTCCTAAAGCTGAACCGATTGCATCCAAGTCTGCATGTGCATGACCATAAGCAATAACTCTTTTAATTTTTGGATCTAAAAGTTTCTTTTCAATTCAATTAGCAAGTGCTTTGATTCTTGCACGGTTGATATTTGGCAAGATTTCACTTGTTGAACCAAAGTAACGAGGTAAATTATTTCTTGAGAAAATAGCTACTTGGTCACCACCACGACTTTGAGCTTGTAATAAAGCATTTTTAGCTTGTTCCATTTTTTCTCATAAGTTTTTTGTTCCATATGCAAAACCTGCAGAAACTGAAATAATAATGTTTTGACTTTTTAGTGTTTTATGTAAATCATCAAAAAACTTAAAGTTTACTTGACTCATTTTATCTAAAGCTTGCTTGTTAGTTACAATAAGAAATTTACCGTTTGTATTATATTGACGATAAACTAAGTTGTAATCACACACTAAGTTATCTAATACTGTAACAACTTCTTTAGTTACATTATAAAGTTGTTCTTCAGAAAGAATTGATTGATAAAGCTGATAGTTATCAATTTCAATTTCACCTAAAACTGTTAATTCATCTTCATATAATTGAAGAGTTCTTTGTTCAAGAGTATTGTCCTTAATACTTAAACAATTTTCAAGTGGTCAAATATTTACTGTATATGAAAAATCCTTGTAATTAAAATCAAAAGAAATGTTATTAGAATCAAAATTAATATTAAATTTCTTGAAAAAATCAGTTAATTTTGATCCGACTCATTTTCTACCAAAACGGTTTTTAATAAATTTACTTGTTCAAAGAATTTGGCCTTCACTGTCAAAAATAAGCACTCCAATTGCATTATGACTAATGATTTCATCAATGAAACTGTTAAAAGATTTTTTTACTATAGCTTGAGTTGATAAAAAACTTTTAACAGATAAAATTCCAAATAATCCAACGATTATTAATGAAAGACATATGCCAAAAATTCCAAAAATGCTGCTATAAATATCTGAACGAGTAAAATACAAAATTACTGACCCTACAGCTAGGAAAACGCCAGCAACTGCTATTAAAGAATATAAAATTATTTTTTGTTTTAAGTTCATAATATAAATTATATATTAAGTATTAATATTCATTTGTAATATATAAAATTTTTGACTCAAAATAAAAATAATCATGAAAAAAACAATAAAAAACTAAAAATGCTATTTTATAGTTCTTTTACTTTTAATTATATTTCTTTTCAATGTTTGAAAGCAAGCTTTTTATGTCTTGCATTTTGTCTTTAAATGTTTCTTGAGTTTCTTCAAATTTGTATTCATTCAAAATAAATTGATAGTCATCTTTGTCAATTTTTAAGAATTTTTTTCTTACTCATTTTTTTGATAACTACAAATTTTAGTATTTTATCATCTGTTGTTTTTCTAAATTCATCTATAAAAAATTGGAGTTTGTTTTCAATATTTAAATCAAGCATTTCATTAAAGGAATTTATAAGTTTTTTAGAATCATTATTATTGTGGTCAATATTAGCCAAAAGCAGAAAATCAGATTTTGATCTAGAAATAACAAAATGATATTCATAATCAGAGTCATCAAAATTGTAATTAGTTCGAAAAACACCTTTGATGTATTTTTCTTTATTTAAAAATTCAACAATGATTTGTATTTTTTTATTATTTATTAAATTATTTTTTGATATAGAAATTATTAATTTATCATATTCAAGATTTTGTAAAAAGTAATCATTATTTTTTAAGATATTTCCATTATTATCAAATACTAAAAAGTCAAAAGAATTAGCAGTTTCTTTTTCAATTAATAATGGGTTATTGAAGTCGATTCAGGTTATAATTTGACCTGTGTTTGCATTTAAATTTTTATTTAATATTTCTTTTAGGGAGCTTTTTTCAAAGATATAGCGTTTGTCTTTTTCTCTAAAAATATACTGTGAATTGTAAGTGGTTAATTTATTATTAACTAGATAAAGCATTATGTTTTTCATTCTATTAATTAAGAAATTTAATTTATTATTTTCATGTCAAGTATTCATATTTTCAAGATGTCTTTTATTTAAAATTATTTTATAAAGATTTGAGTTTTGTATATAGAACAAATTGTCGTTATTAACTGTAAAATAGGTTTTTTTGTAAAAAGTGTTATCTTTTGAAAAGCATTTGTTAAGCTATCTATAAAGTTTAAACGATCTGAAAAATTGTTATAAAATTCTAGATTTGCTTCAAATTTTTCCAATCTTTTTCAATACAATTCATAACTAGAAACAAAGTTATTAAAAGCAAGAACAAAGTTGGTATGAACCTCAAATATTGGACTTTTAATTATGTTTGGACTTATTCCTGTATTGAACAGGGCTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTTCTAAAGCGGTTTTAAATTTATTTTCTTTCCCAAATCAAAATTCTCTTTTTACAACACTAAATAAACATTCAGCGGGACTATTGTCTAAACAATTTCCTTTTCTTAACAAGCTTTGAATTGTTTGTTTCTTTTTCAAATAATTTATATACTCTTCATGAGTATATTGTCATCCTTGATAAGAATGCAATAATACATTGTTTAAGCTATGTCAATTCTCTTCTACGTTTTCCAACATTTTACCCGCCATTCTTAAGTTAGTTGATTTAGAAATCGAATAACCTAAAATCTCTCAACTTTTAAAATCAACCACACGAAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTATTCCTAGTATTTTCAAATATTTATCACTAAAAAAATTTTTTTGTATCTGAAGAAATATTTTGCGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTTTGTGAGTATTGCCTATGAATGAAGAATACTTATTTTTTTCTTAATGTTTTGCCAAAAAGATTTAATGATTTTATTAATTTTTGAACCTTTTTATGATTAATCATTATTCCTTTATTTCTTAATGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAATATAATCTTTTTCAAATTTTCATCTTTGTCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAATTGACTTTTTCTAATATTAATTTAATAGAAAAATAACTTTTTAAATTCTTGCCTTAGTTCAATTATTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTTAATTGAACCAAAAACGCTCACTTTTTTTGCTATCGCGTTTTCCAATTTAAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATCTTCCATTTTATTTTCAATATTGATTTTAAAATTTGTTATTTTTTTAAATTTAAATTGAATAAAAATGAACCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAAATGACAAAATTAAAAACATTTTTTAAATATTTTAGATGAATGCAAATTTATCAATTATATAAATGTTGGATATGACTGTAGTAAATTAATTTTAATTTTTCAAAGTAATGTTAGTCAAGATAATTATTCAAATAACAAAGATAAAAATAGCCAAAAAGAACAAGAAAAAATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGAAAAAGCTATTTCAAATTTATTTATCGATATTAAGAAATCAGATAAATTTTATGATGATGAATTGAAGCTTAAAATTTATGCTATTAAAAATGATGGTAGTGGCAAAGTATTCAGAACCAAAATTTACTAATTTTAAGAATTTTTCAGAGCTTATTTATAAAAAATAGGCTCTTTTTTATTTGCAAATAATATCCACTATTTTTTGTTGTATAATACAAAAACTATGGATCAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGACAAAGAACATTCAAAAAAGATACAGCTAATAAAATTCAAAAATTTGCTGAAGAAAATAAGATTTTATATGTAATTTTCGATGAAGAAGGCGAAACAATTTATGTCCATAATGCTTTTGATTATTGAATCAATAAATTGTTTCTTTCTAAAAAAATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCACAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGTTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTAGGCGCTCCTCAGAATGCTAAAAGAGTTGATCCAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAATTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGAGATGCAGGTTATAATATTAAAAATTATCAAGAATTTAAGAGTGAAATTAATAAATATAATTTACTAAAACATATAAAAGTTATTCATTTAAATGATTCAAAAAATGATTTAAATTCACATAAAGATCGCCATGAAAATATCGATAAAGGTTTTATTGGAATTGATACTTTAGCTAAGTTTGTTCATGATAAAGATTTTGATAATGTGCCAATAATTTTAGAAACTCCAATTCCTGAAAATGGACCTATTTATGATCAAGAAATAGCTGCTTTATTAGCTTACAAAAAATAACAAAAACACCGAGAAATTCTCAGTGTAATTTTGTGCTTTTTTACCGTATTTTTACCATGAATAGCTTTTGCATTATTTAATGTACTATTTGCAATTTCATTAATTGATTTATAGTTTTCATGATCACTAGTTTGATGAATTTAAATTAATTCATTTAACTTTTGAATTTCTCCATTCAATCATTCCTTAGTTTTTTTAATTTCATCACTTTCAATATTAGGTATTTCAGGTTTCTAAGCGCATCCTGTTGATATTGCTATTGTTGGTAAAATTGTTGTTGAAATAGAAATAGCTACAACAAAAAATGTTTATTTTTTTATAATAATTTGCCTCTATTTTTTTCTATTATCACAATATAAAATGTATAAAAATTTAATTCTTTTCATAATAATATGAAACAGTTTTTGCAACAATTTATTAAAAAAATTACCATATTAATAAAACTAAAAAAATTAAAAATAATGAAATATTGAAATGTTTTATTTACTAATAAAAAGGTTACACATTAATAATAAATACTAAATTAGATATTAATATTTATATAAAAATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGATTTGGTTCAATATTCAAAAATCATTATTTTGCTTGGTTTAAATCTTATACAAGAGTTGATTACTGAATTTTAAGAAAAAGAATCATCAGCTTTGTTTGGTTAACATTATTATTTGTAGTTTTAATTTATCATGTGGTTTTAGTTAGCTTCCGTGTTGAAAACATTTGATATTATTCAACAGCTGATGTTAAAAATATTTACTTAAAAGGTTGATACTATACTTTTACAAATCAAGCTGATTTATTAGATGGTAAAAGTGTTGATTATCCAGTTGCTTATTTAAATATTGGTTTTTTACTTGATACATTATTTAATCTTTTTTATGTAGTAACTTTTAGTCCTTATTTAGCAATAATTAGTGCATTTTTAATTTGAACCTTAGCTTGATTATTCCTTGTAACATTAAATCCAATTAAATATTTTAAAAACATTAACGAACACAAAAAAACAATTGTTATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGAGTTAGTCCAATATTAAGTGATAATTAGGAGTAAAAATGAAACAAGTTGCATGAAATGAAGTACAAAATATGGTTGCTAATAGCAACAACGAAAACAATATTTACTTTATAACCTTTACAACTTCATGATGTCCAGATTGTAAAGTTATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGAAAAATTTTACATGTTCCAACATTTTTAATAATGAAAAATAACAAAATCATTCAAAGAGGAGAAAACTATTATCCAAAAGAAATTCTTTGTGATTGAGTAGAAAAAGTTTTAGAATAAAAAATGACTAAAGAAGAAATAATTACTTCATTAAAAAATGTTTCAACAGCACCTGGTATTTATCTCTGAAAAGATAAAAATGATCAAGTTATTTATGTTGGAAAAGCAAAAAACTTACGCAAAAGAATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGACAAGAAAAGTTCAACAAAATTAAATCCATTTTAAGTTTTAAAGATAACTCTTATATTAATGAAATTAAAGAAAAAATGTTCAAAGAAGCTGAGGCTGAACATTTTGAATTAGCTCGTGATTTAAAACTTTCCTGAGAATATCTTAAAAAGCTTAATGAAGAACAAATTATTGAATTAAAAGATATTCACAATTTAGACGTATTTGCCTATAAAACAGTTGATAACATAATTTATATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTTAAAACATATGTTAATATCTTTAACGCTCCTTTAGAAGAATTAGAAAAAGTTGTTTCTAAAAAAATAGCTTTAGAAATCAAAAAACTTTATGAAAGTAAGAAATAAGCAAGTTTATTTTGCTTGTTTTTTTAATCTATTTTTTATTTACAATAAAATATAAATAAGAATAATAAGGAGATTTTATGAGTTTTAGTAAAAAGTTTTTATGAGGTACTGCTTTTGCAGCTAATCAAATTGAAGGTGCTTATAATGAAGAAAATAAGGGTTTAAATGTCCAAGATGTATTACCTAAAGGTGCTTTAGGTCATATTGATTTAAATTTAAAAGAATTTAATATTAAAAGAAAAGCTATTGATTTTTATCATAAATATCAAGAAGATTTAGATCTTTTAAGTGAAATGGGAATTAAAGTTTTAAGAATTTCAATTGCTTGAAGTCGAATTTTTCCAAAAGGTGATGAAAGTAAACCTAATCAAAAGGGTCTTGAGTTTTATCTTAAATTGTTTAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAGTAATAAAAAAGTTGTTAAATACTTTTTAAAGTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTCTGCTAAAGTCAAAGAAATGATTAAAAATAATTACAATCATATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGCGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGACAAAATCTTTATTTTACTGATGTTTTAGCTCGCGGCGTTTATCCTAAATATTTAGATAATTATTTTTTAAAAAATAATATAAAAATTAATAAGACTAAAGAAGAACTGGAAGTGATTAAAAATAATATAGTAGATTATATTGGTTTTAGTTACTGTTAATTCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTTTTTAAATTGTCCTTTATAATATAAATTGCTTAAAAAATAAGAAAGGACACTTAAATTATAATATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGAATTTCTAACAATTATTTTAGAAATAAAGTTCATATTATTTTCAAAAGTGAGGGTGGTAAGTTTTAAAAAATTATATATGTGATTATGTTAATAATTTAACAAGTTCATTCAAATTTTAGTTAATTTATTGAATTCTTATTTAGCTTCTCCATATAAGTCTACATTGAACTTAACATTGTTTTTTAAATCTGTTTCATAGTTTTTACATTTGAAATAAGTGAGAGGAAATAGAAACAATCCAATTAAATATATTAAGCAAACTAAAATAAAGTTAACAACAAGTTTCATAAAATAAACAAAGATAACTCATTTAACACTTCCTCAAGCTATTATGCTACTTCCTCTTCATCTAGGATAAAAACAATCCATATGATAATAAGTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGAAATACTCCTATTTGATAATTTGAATAAACAAATTATAAAATAAAGAAATAAAAACTTTATTTAAATATTTAAAAAGCTTAATAAAAATTAGATAAGTAATTATGTTAAATACTTTTTTTAAAATTCTTTAATTATGTCAGGATTTTCTAAATAACTTAATAAAGAAACATGAGTTATTCCATCTTGATCTATTCAGTTATTTTTTTCGCTATAATCAACAATAACTTTTCTAAAGTTGTTGTTAGTTAAAAAGAATGGTCTCAATTCTCTTTCTTTAACTTCTTGATTGTTTAAAGAAGTTGTTACTTGAAAATATACTTTTGTATTGAAATTTTGTGCTATAAAATCTATTTCATATTGATTATTAATTCATTGATTATTAATATTATTTTTTTGATTAATTGCCCCATAACTAATATCGAAGTAATTTATTTTTAGAGCATTAAAAAAGGCATTTTCGATTAATTGACCTTGTCCTAGATTTCTAAAATTGAGAGCTACATTTCTTAATCCGTTGTCAACAAAATAGTATTTATATTGACCCGAAAGAAGTTTTAAACCTTTAATATCATATTTTCTTATTTTATCTATTAAGAAAGAATCTTCTAGTGCATCTAATATTTTATCTATTTGATAAGTAGAAGTCTTTTTATTATTTAAAGAATTAAAAGAGTTGGCAATTCTACTTGAATTACATTGAGATGAAACTATTGAAGCAAGATATTCAACTATGTTTAAAACATCTTGAGTACTATTAAGTTTATGTCTCTCAGCAATGTCATTAACATAAACATTTGTTAAAAGATCTTTTAATATTTGTTTCTTTTCGTTTTCCTTATCTACTAAAACTACTTCAGGCATACCACCATAAAGAGAATATAAATTTCAAGCTTCATTTATATCAAGATTACTAAAGTTTAAATATTCTTGAAATGATAAAGGCAAAACATTAATCTCCATACCTCGGCCCGCAAAAGTAGTAGCTATATCTTTTGATAAGAATTTAGAATTACTACCAGTAACATAAATATCAATATTATCTTGATCTTGAAATGAATTTAAAACCAATTCAAACTTTTTGACATTTTGAATCTCATCAATTAAAAGAAAATATTGTTTATCATTAACAATTCTGTCTTTAATGTATTTGTATAACTTTTGACGATCTCAAAGTTCTTCATTTTCTACTCGGTCAAGTTTTATTGTTATTATTTGGTCAGCTTCAACTTTATTAGCTAATAAATAACTATAGAATAAATTGAAGAGTAAAAAACTTTTACCTACTCTTCTTATACCTGTAATTATTTTAATTTTTTTGTTATTGATTTTATCAATTAGCATATTTAAATAATTGTCTCTTTTAATAACTTTATTCATTTCTAACTCCTTATAAAAAATTATACTATTTTTTTAAAAAGTTAGCAATATTGCAAATGTTTTAAAGATTTTACCTATTTTTATATACTTTTCGTTAAAAAAATAAGCAATATTGCAAATGTTTTAAAAAAATTACTTACTTGTTTTTTAGTGATATTCTATTTCACATATAGGTGGGAATGCGAAGTGATAAATTTTTAAAGATTTGACATGTCCGCATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTCCAGTTTTAAATAAATACAAAAATTGTAGAAGAATAATAGTTAATGATAGTATGCACTCGTAGTCCTATCAAATCATTCATAGATACAAGCGTCTATTTGTAATTCATCACCAAAATGCATGTTTTCTTTTCTACACAATCTAACTCTTAGTCTCGCTTCGCTAAACAAACATAAAAAGTTTTGATAATTGTTTCTAACTTCTTCTATTTGAACTTTTAACAATTCAGCAATTTTAATCATTTTTCTTACTTTTCTTCTAGTTTTTTTATGACTAAAAATGGTTTAAAATCTTAAGCTTGTTAATCTGTTAACTAAAGCTTGATAACTAATTTGTTTTTCATAGCTTTCATGAGAATAATTTCCAACTCTTATTTCTTTTCAAAAAGATTTAATTGTTGCATCATTAACATTTTTGATTCCATTAGTTCTATTTTTAATATATTCAGTGTATTCATCCATAAAATTATCAACTGTCTGATTTAAATTTTTCTTGATTGCATTTTTATTTAATCTATTTTTAGAAACAATTTGAATAGCGTTGGTTTTTCTATTTAAAAAATCTCTAATATGGTTTTTGTATCTTTGAATAGATCTAATTGATAAATCAACTTTTTTAGCAATTTGTCAATTACTCAAATTCATTCAAGATGCAATTAATTGATAGTTTTTTTCTTTGTTTTTTCTAATTCTGTTGGATTAAAAAAAACTAAATACTAAATTCATAAATACCTCATATTTACAATTATAAATGTCAAATTGTTTTATATCGAAGGTATGACAAACTGTTTTAGAATTTATACTTATAAAATTAAAATGATATTTTTTGCATATATTGAATTATGTTAATTTATATTTAGCATTTTTATATTGACCAACTTTTTTGATTTTATTTTGTTCTAATAAGACTTTAAGTTTTCTTTCAAGTGTTCTTCTTGAAATTGAAACAAGTTGTTCACTTATTTCTTTAGTTGATAAAGGAATTAAGCTAGTAGTTAAAATTTTGATTATTTGATTTACTAAATAATCTTTTTTATTTACATCTATTAATTTATTATAGGAAATAATTAAGCAATCTAAAAAATAATCAATAAAAGGCAAATAATCGTTTTGGTTATTGTGTCAATTAGTTGAGCTTTTTCTTAAAGCAAAATAATATTGATTTAATTTTTCTTCTATGATTTTTTCAAAGCTAATGTATTTATTAATCATAAAATCATTTTGAAATAATAAACAGTTAGTTAATAATCTTGAAATTCTACCATTGCCATCACTAAAAGGGTGAATACATAAAAAATCCAAAATAAATGTTGGAATTAGTATTAAAGGATCAATTATTTTTTCATCATTTCTTTTATTGTATTCATCACATATTTTTGTTATGTAATCATCAACCAAAGAATGATCAACCGGTTCAAATATAACAGTTTTATAGTTGTTTTGGTCAATTGAAAAAATATAATTGTTCATTTCTTTAAACTTTCCTTTTAAATAAGTTTTATTACTATATTTATATAATTGATTATGTAAATTTAAGATGTTTGATTTAGTAATATCTATATTATCAAAATTATTTAAAATAATATTTAATACATCATTATAACCTTGAATTTCTAATTCGTTTTCGCCTGTATATTTTTTATTATTAAAAATAATATTTTCAATTTTGTTTGTAGTTGAAACAATATTTTCAATTTTGTTTGAAGAAGTAACACTTTGAAAAATACTATTTTTAATTAAAGTATTTTTAGTTTGTTCTGGAATATTAGAAAAATCATTTATAAGTCCATTTAACTCATAAATTTGTTTTAATTTTTTGTTAATTTCATTATTAATAATGTTCTTTAAAAATGAATAATCAAAAAACCTTTTTTCTTTTTTAACCATAATAATTAATTATAAACTAAAATATTTTTTACGACATTTTTTAAATTTTGTCGCTAAAGTTAATAATGTTGTCGTAGAAGTCTAAAATGACTTTGAAAAACATTTTCGAGGGGCATTTTTCAAAAAACGTGTTTCTAATTCTTGCCAAACAATAAATAATTTTGTAAATAAATGGTATTAAATACCAAAATGAAGCACTTTTTATTAATGTTATGTAAAAAACAAACAAATTTGTGGTTTTATATTACGATATATTACATTTTAATTAAACAAATGATTTTATGTTTTTAAAAAAATCCAAATTTTATCAATAAAAAATGTCCTAAAAAAGTCACTTTTTCGAGGTACATTTTCGAGGGACATTTTCGAGGGACATTCTTTTGTTAGGTATTATAAGGGCCTATTTATCAATAATTTCCCAATAACCATTTTTACCTTTTCCAACATATTTTATGTTTTTTATTTCTCTAATATATCTTGAAATAGTTTTTTCAGTTAAATTTAAAATTGCTGCAATTTCACTTCTTTTTATTGTAGAATTTTCTTTAACTAAATTGAAAATTCTTTCAATATTTAATTGTTTTTTGTTTGTAATATTTTTATTATCTGTATCTATATTAGAGGTCATTTTACCAACTTTTAATGTAGCAATTTTTGGAATAGGAATAATCGTTTTAAATATGTTACTATCCAAAAATGTAGGTTTATTATTTGAATAAAGTTTTGTATATTTATAAGTTTTTCTAATTCCTGAACCTAATTCATCAGCTAAACCAATTTCTCTAAAAACTTTTGAAATTGGTGGATTTTTTGGATTTGGTCTAAAGGTTTTAGGATCTAATTCCTCTTTACTGCTTGAACTATTATCATTTTCAACAGTTATATTGTTTTCATCAATGACAATAGTAGCTGTCATATTGCTTGCATAGTTTCTATGAGCTAGTGTATTAGAAATGATTTCTCTAAGTATCAAATTACGAGCACTAACACTTACAATACCATCTAAAACAAAAATATCACTTAAGTGTTTTTCACCAAAATTAATTAATTTCTCATAGCTATCGATTAAATTGGTTGCTACTATTTCTCTATCATCATATCTATCTTTATTTTTAACTCTAAAAATAGCATCTGTACGATATTGAGGAAGAACAGACATAATAGTTTGATCTTTTCCAAAAAGCAAAATAGCTGCTATAGTTATTCCTTCTTTATTGGTTTCAGGATCTTTTAGAATTAAACCTGCGCTTTTAAGCATTTCCTCATCATTTAAGTTTTTTCACAAATGATTTTGATTTCTAATAATTGCCATTTTTCTTGCTTTATTAATAACTTTTTTGTCTAAATCATTAATACTTAAATTAGGATAAACTTTGTTTACAAAACAAATACTTTTTTTCTTTTGAAATAGTTTTCAAACTAATTCATCTTTATTAGTGATATCAACATCACCTTCAAAAGATCTTTCAAAGATTCTACCATTATGTCAGCAAACATTTACTCCAACTGGAACATAAATATAAATTATTTTTTTATCATCAAAATCTATTACTTCAGGTTCAATAAATAAAGGAGGATTAATTTTAGTTGAATCATGAATTGAATTCAAGAAGTCATTTACTATTTTTTTAACTTTAGTTTTGTCTACACCTAAAACTTCACCATTGTCTCCAATACCTAAATTAGATGACCTCCATTTTTATTGCTAAAAGCTGAAACGGTATCAAAAACACTTTTAGATAAGGCATCTTTGCATGTTTTGAATTCTATTTCTTCGCCTTCACCTTGGATTAAAAGTTTTTTCAATTCTTCTTTATTCATAATATCTCCTTATATTAAATATTTTATATTATTTATACAAATCTATCATCTAAAAGAAAATCATATAAACCTATTACTAAAATATTATCTTTAATAAAAGATTTTCCTCTAAAGTTCTTAACAATTAGTGATTGTAAAGTACCTTTTTCAACTTTTTTCTAATGGTCTTATTTTTCTTTCTAATACACCAGGATTATTAATGTCATAACAACATTGAAATAAACTTTAGTTATTGAATTTGTCACTACAAAATCAATTTCTAATTTTTCTATTTTTTGTCTTTAGTTCAAGTTTTCGATTACACCAGCATTAACATTGTAACTTTTTCTTAATAATTCAAAGTAAACAATGTTTTCTATGTTCGACCAAAGTCATCGAATTTAAAATCATTTAAAGCATTCTTTAAACCATTATCGATAAAATAATATTTTGAATTACTATCGTTTAATATTTTTAAACCATTAATATCATATTGAGTAACTTTTTTAAAAATAAATGATTCTTAATAATAGTTACAATAAGTATCTATTGTTCTACTATCAATTTTAAAATTGTTATTACTATTTATAAATTACTTATTTTTTAGTATTAGTTAAAGATGAAGAACTAGAAGCTAAGAATTTTAATAATTCCTCTATTTTATAGTCATTTTTGACATTATAGTGTTCTAATATATCACTTAATAAGAATGTTATTAAATATTCTTTTAAATATTTTTGTTTGTCATTGATGTTTTCTTTTAAAATAACTAATAACATACCACCTTATTTATAATAATCATCAAAATATAAATCATATTCATTTTCTTTTAAATTGAAATATTGAACTATTTCTTTAAATGAATATGGACAGATTTTTATTTCTTCACCGCGACCTATAAATTCTATAGCTATATCATTTGAAAGAAATTTTAAGTTACTACCAGTAACATAAATGTCAACTTGACTTTTGACTAATCTAAAAGTGAATTTAATAATAACGGAAAGTCTTTAATGTTTTGAATTTCATCAAGTAATAAATAATATATTCCTTGATCTTTAATTAATTTTTTAATATGATTATATGCCTTAGTGGCATCTAATAATTCTTTGGTTTTAAGGTTATCAAATGATAAAGAAATGATATGATTAATATCTACGTTGTTTTGAATTAAGTAATTATAAAAAAAATATTTAATAAAACACTTTTACCACTTCTTCTAATACTAGTAATAACTTTAATTAATCCATTGTTTTTATATTTGATTAATTGATTCAAATAAAAATAATGTAATTTTGTGAAATTTAACCACTCAATTACATTATAGTTCAATTTATTTTTAATCTATAAATCCTAAATCACTAACTTTAAATTTTGCATACTTTTTATTTAATTTATAGGAAGTAATTCTTTTAGTACCACTATTTGGAACTAAAATATCTTTGTCTATTCAGTCTTTAGTTCATTTTGATATAGCTCTAGAAGTAACGCCAAAGACTAAAGCTAAATCTTTAATAAGAATAATACTTTTTTTATTTTCTATGCAATATCTTAATAAAGTTAAGTCTTTTTTATTAAGATTATTTAACAAATCATTATAACTATTATCTTTAGCGCTTGCTTTTAAAGCACTTAAATATATATTTTGTGCATTAAGAGCCATAATTTTACAAAAGTAAGTTATTCATTTTTCTAAATGTGGCGGTTTATTACGTCCATTATAAAATAAAGGACTTAAATCCATTTGTAATGCTTGATAATATTCATTTAAGTTATTACTATAATATTCTTCAAAAGAATTAAATCCTTTGAAATCATAATCATCTAACATTAATGAATAAGTAGCTAGTGCTCTAGCACTACGACCATTATCATCTTCAAAAGGGTGAATTGTAACAAATGCATAATGAATGATAGCTGCTCTTATTGCACTTGGTAAATGTTGATTTTCATTTATTCAAGTTATTAATTCATCTATTAGAATATTTATATCCTTAGCTTCTGGTGGTATATATTCAGGACTTTTAGTAACTGAATCATATACAGCAAAAAGTACTCCAAGAGGAGTAGGTTGTCGAAAATCTATTTTGGTAATTCTGGAACTTTTAGCTTCAATAATATTATGTAAACTAAAAATAAAATCTTTAGTTATTTTTATTTTAGCTTTTTTACTTCTTTCTAAGAAACTTAAAGCTTCTCAATAATTTAATACTTCTTGTTCAGCTTTAATTCTAGTTTGATGTGTTTTATTGTTTATAACGTTTTTAACTTGTTCAATGTTTAGAACATTGCCTTCAATGCTTGTAGAAAAATGAGTTTGTTTGATTTTTGCTTCAAATTGAATTCTTTGTTTACTTCTTGTAGGTAAAGACAAAAATTCTAATGAACTTTTATATTTTTCAATTTCTAGAAGATAATTGACAAGCTCATCTGTATATTTAAATGATAGATCTAATTCCTTTTTCATGTAAATATTATACCAAATAAGGAACAAATAGAGAACAAGTAAAGAACGGTTTTGTTCTTAATTTGTTAAACAAAAGCATAAAAAATCTTCCCTAAATAATGAGAAGATCTTTTTATATTATTGTTTACCTCTAACAAATGTTATGACAAGGCATACTTTTCAATTCATTCAATAATTGACAGCTAAAACTTCTCAACCTTTACTTGCCATTTCATTCATTAAATCTTCAGCTTTTGATGGGTGTTCTGTTAAAATTTTATATTCTTTCATATAGACTTTCCTTTATTAATATTTTAGTATTATTTTAACAACTAATAATATTATATATTAGAGCGAACCTTTTTCCAAAAAGTCCGGAATTTGTGGTTCACTATAAGGTTATTAGAACTAATAAAAGAAAAGAATTTTTTGTTTCTGAAAACACCACAACCCCTTTAACTAATACTTTAATGGAAAAAAATAAAAGTAGAATCTTCAAGTTCTCCTACTTTTAAAGTACAAGTTAAAAGATCTTTCAAAACAGCTCAAGCTAATTATTTACCAATACTAAATGGTAAAAATTTAGAAGAATCTAATAAAATTGCAAATGACCTTAACAACTACTTTAATGAAAAACTTAGAAAAAATATTGAAGTAAAAGAAAATCATTTTGGAAAAGTAATAAATGGTGATTATAAAATCATTGAGAGATTTATAAGAATAGTAACCGCTAACACAATACAATATGATTACAAATATTATTATCCTTACAATCCAGAAAATGGTAAAAGAGTTTTTCTAAATGATAGATCAAAAGTGTATGTGCTTATTGATAAGGATGGTAGTATTTATATCGACATTTTAGGAATTGAAAAATTTCCTACTAAAACATTGACTAGAGAAGAACTTAACGAATATCAACTTTTTGTACACAATTTAGGTGTGACAGAAAGCACAATATCAAAACTTCTTTATACAAAAATCAAATCAACAAATAGTTTTTATAACTTAATAGAATCTAAAATTGAAAAATTAAAAAATCTTTTACTCACTCTAAATTTAGACAAAAATAAATTAGAAAGCGAAATAAAAGATTTTACTCATAACATCTGTAAAATCAATAAAAAAATAGGTGAATTAATTCAATTCTAATTTCAATTATTTCTTTCAGATGACAAAAATATACTCTTTTTAAAAAGAAAGAGTTAGTTTTTTGTACTAAAAAATTAAATTTTTAATTTTAAACACTGTTTTATTTTTTTAACTTTTCCTATAAAAAAACATGTTTTTTTGAAAAAAATTGACAAAAATTTAATAATTTTGTATCATTAAATTAATGTCAAAAAAATATGAAAAAAAGTATGACAATCCGTTTTAAATTTCACATTTTGCTTTGTTAAATTTTATAAAAATTCATATATGCTATTATGTTAAAAAATAAGCAATATTGCCAATGCTTTGACAAAAATTGCTTATTTGATTTGATTATTAATCTTCTCAAATTAATTTGCCTTGAATTCTTTCTCTTTCTTTATCTATTTCAAGGATTTGGATTTTAATGTTATCTCCTGTTTTAACTACTTCGCTAGAGTGTTTGATGTAGTGATCTTTATTTCTTTTCATATTTGAAATGTGAATTAAAGCAGCTTGCTTAATTCCAATATAAACAAAGATACCAAAGTCTGTAATATTTTGCACTGAACCATCTAGAATCATACCTGGTTTTAAGTCTTCTAACTTAAGAACATCTTTTTTAAGAATGTAGCCATCTTTGTCATCACGAATATCTTTAGTTGGATTAGATAAAGAATCTAATATTAACTTAACATCATATTCATTTGAATTAAAGTCTTTAGCTAATTTATTAACATCTTGTTGTTCAATAGTTTTTAAGTCTATATCATTTAGATCTAATTGAAGCGCATTAACAATATTAATTGCTAAAGGATATGATTCAGGGTGAATAGCTGTGCGGTCATAAAAGTTTTTAGAATCATGGATTCTTAAGAAGCCAACCGCTTGTTCATAGGCTTTTTCGCCTAAACCTTTAACCTTTAATAGTTCTTTTCTTTCTTTAAATTTATTGATTTTTTCACGATAATCAATAATGTTTTGAGCTAATGTTTTAGTTAAACCTGAAATGTAGCATAAGATTTCTTTAGTAGCACTATTTAAGTCAACTCCAACCATGTTAACTACTTTATCAACTTTAAAGTCTAAAGCCTTAGATAAATCTTTTTGATTAACATCATGTTGATATTGACCTACCCCTATAGCTTTAGGATCAATTTTAACTAATTCATTAAGAGGATCTTGGAAACGACGTCCAATATTAACAGCTGATCTTTCTTCAACACTAAAATCTGGAAATTCATCAATAGCAACTTTAGAAGCTGAATAAACACTAGCTCCTACTTCACTAACAACAGCATAAGAAATATCTTCATTAGGATTTACTTTTGATCTTTCTTTTAATAAATTAGCAATAAATTCTTCAGTTTCTCTTGAAGCTGTTCCATTGCCAATAACAATAATATTAACTTTGTATGAATCTAATAGTTTATTAACTATTTCTTTAGCTTTATCAACATAATTTTGAGGAGGGTTAGGATAAATTAAAGCTTTTTTTAAGAACTTACCTTGAGGATCTAAGATTGCAATTTTGCAACCATTAACAAAAGCAGGGTCTATTGCCATAACTGTTTTATTTTTAACAGCAGGTCATAGTAACATTGTTTCAAGATTAGTTGCGAAAAGGTTAATTGCTTCTTTTTCTGCACGAGCAAAAAGATCACTTTTGATTTCTCTAATAATTGAAGGTAAAATCAAACGATCTAAACTGTCTTTTAATGATTCGTTCAAAATCTTGCCTGTTCTTTTGATTTTAAAATATTTGTTGTTTAAGTCATAAGTAATTTTAGCTAAATTATAAGTCATGTCATAACTTAAAATCTTTTTGTCTTCACCACGACTAATTGCTAAAACACGATGGTTAGGAATAAACTTGATTCTTTCTTTATAATCATAGTATTGTTGAAATACTTCTTTTTCATCTTCAGCATTCTTTTTAACTTTTGTTACAATGAAACCAAAATCATAAATTTGTTTTTTAACATATTCTCTATTTTCAACATCTTGAGACATAATTTGGCTAATAATGTATTGAGCTTGCTCAATAGCAAATTCAACTGTTGGTACTTTGTCATTTAAGTATTTTTTAGCTTCTTTATAAGGATCAAAACTTTCATCAGTTGCTTCCATAATTGCTTTAGCTAAAGGTTCTAAACCTAAAGCAATAGCTTCACTAGCTTTAGTTTTTTTACCAACTTTAAATGGTTCATAAATGTTTTCAACTTCAGCTTTAGTTTCAGCTGCTTTTAATTTATTTTCAATTTCAGGTGTGAGTAATCCTTTTTCTTTTAAGATGTCAATAATAGCTTCTTTTCTTTTGTTTAATTCAACATTGTAAACATACATGTCACTTATTTGTTGAATAACTTCTTCATCAAGTCCACCAGTTACATTTTTACGATAACGGGCAATAAAAGGTACTGTTGCTCCTTCATCCATTAACTTAAGAACAACTTCAACTTGGTCTTCTTTTAAGTTTAATTTTTGTGCTACAAATTTTTTAGATAAATCCATATTTTCCTCCAATTAATTAGTATGTTAATTATACATATAATTTGCATAAAAGTAATTTAAAAATGTAATCATTTTTGACTAAAAAACCTGTTTTAATAATTGATTAGAAAATAATTTGTAAATAGTGATTTCAAATCACAATTTTTTTCTTTTTTTTAACTAAAACAACTATAAAACAGTTATTTAGGCAAAATCAGTGAAGAACAAAAATAAACAAAAAAATTATTTTATTATTAATATAAATATTTAATATTATAATTAAGTCTATAACTATTTAAATCCTATTGTATTAAGGAGAATTATCGTGACTAAATTTATTGTGACAACTGGTGGAGTTTTATCTGGTTTAGGTAAGGGCGTTACCAGTGCAAGTATTGGAAATTTATTAAAGGCACAAGGCTTTAAAGTCTTTGCTTTAAAACTTGATCCATATTTAAATATTGACCCAGGAGTAATGAGTCCCATTGAACATGGTGAAGTATTTGTAACTGCTGATGGTGGTGAGACTGACTTAGACTTAGGACACTATGAAAGATTTATTGATGTTAAGCTAAGCAAGGAAAGTAACTACACAAGTGGAAGAATTTTTACTCGCATTTTTGAAAAAGAACGTCAAGGACTTTATGGTGGAAAAACTGTTCAAATAGTACCTCATGTTATTGATGAAATTATTAGCATTATCGAAAATACTGCTAAAAAACAAAAACCAGATTTCATGTTAATTGAAATCGGAGGAACTGTTGGAGACATTGAAAGTAATCCTTATATTTATGCAATTAGTAAGTTTGCTAGTTTAAATCCTAAAAGTGTTTTCTTTGCTCACTTAGCATTTATTCCTTATTTAACTGCTTCAAAAGAATACAAATCAAAACCATCTCAAGTTTCAATTGCTTCTTTAAGATCATTTGGAATTAACCCTAATATTTTACTTTTAAGAAGTCAAGGCGAAGTTGATGAAAGAATTATTAATAAAGTTGCTGAAGCTTCATTTTTGGACCCTAATCATGTAATTGGAGTTCCTGATAAAAGCAGTATCTATGAAATTCCAATGTTCTTATATGAACAAAATATTTTAAAAATAATTTACTCACATTTTGAAATTAAAAAACAAATTAATGACAAGGTTTATGATTCATGAAAAGTATTTTTGAATAAATATCACGCAACTAAAAATTATAAAATTAACTTACTTTTAGTTGGTAAATATACTGAATTAGAAGATGCTTATTTATCAATTATTTCTTCATTAAAAATAGCTGCTGTTCATCAAAATGTCGAATTAAAATATGACTTAATTGATGCTGATTTAGTTAACAATAAAAACATTGAAAAATTAACAAAAGCACATGATGCAGTAATGATTTTACCAGGCTTTGGTGCTAGAGGTTTTGAATCTAAAGTTAATGTTACGACTTACACAAGAGAACACAAAATTCCTACTTTAGGTATTTGTTTAGGTTTTCAAGCAATGTCAGTAGCTCAAGCTCGATTATTAGGCATTAAAGATGCAACCAGCAAAGAATTCGCTGAAAATGGTAAAAAACAAACTTTTGTTTTAACTCCTTTTTATGAAAATGGAGACAAAATGGCTATTGGTGGCACACTAAGACTTGGTGAAGATCAAGTTCAAGCTAAAAATAACACCTTAGCTCAAAAAATTTATGGTTCAAATATTTTCTATGAACGTCACAGACATAGATATGAAATTAATCCTGAATACTTAGAAAAATTACAAGATGATGAATTTACATTCAGTGCAATTCATCCAACTAAAAAAGTGGCTGAAATTTGTGAAGTTAAAAACCATCCTTTCTATTTAGGTGTTCAATATCACCCAGAATTTAGTACTAGAGTTTTAGCTTCAAATCCACTTTTTGATAATTTCTTCCAAGCAATTATTAAAAATCAAAAATAGGAGGTCAATATATGGCAATTGATTACAAAAAAACATTAAATATGCCTCAAACTAATTTCGATATGCGTGCTAACTTAACAACTAAAGAGCCAGCATTCCGAAAAGAATGAGAAGACAATAAAATTTATGAAAAAGTATTAAATAAAAACAAAAAGAATACACCTTTCATTTTGCATGATGGTCCACCATATGCTAATGGTAATTTGCATATCGGACATGCTTTAAATAAAATCCTTAAAGATATTGTAATCCGTTATAAATCTTTAAGAGGCTTTTATGCTCCATATGTTCCAGGTTGAGATACGCATGGTCTTCCTATTGAACACAAAATGCTTGAGGAAGCACACTTAAAGAAAAATGAATTAAGTGTTGTTGACTTACGTAAAAAAGCTGCTCTTTATGCTCAAGATCAAGTTAATAAACAAGCTGAACAATTCAAAAGCATGCAACTTTTATGTGACTTTAAAAAATACTACATTACTAAAGATCCTAAATTCGAAGCTCAACAATTAAGACTTTTTAAAAAGATGATTTTTAGTGGTCTTATTTACAAAGGATTAAAACCTGTTTATTGATCACCTTCAAGTCAATCAGCATTAGCTGAAGCTGAAGTTATTTATAAAGATATTAAATCACCTTCAATTTTTGTGGCATTTGAAATTAAAGATAATTTAGGTAGCCAAAAAATTAATGTTAAAGATAACTTAGTAATTTGAACTACTACACCTTGAACTTTACTTGCTAATGCTGCTGTAGCAGTTGGCCAAGATATTCAATACTCAAGAGTAGAACATGATAAAAAAGGCTATATTTTTGCTTCTGATTTAGTTGAAAAAGTAGTTAGTCAATTAGGCTGAAAAAGCTACAAAATCACTAACACTTTTAATAGCCAAGAAATCTTAAATATGCAATATGTAAGCCCACTTAATAAAAATGTATGTCCTGTAGTTATAGGCCACCACGTTACTTTAGAAAGTGGTAGTGGTTTAGTTCATATTGCTCCTCTTTTTGGTGAAGATGACTTCCAAATTGGAATCAAAAACTCACTTAAAATGATTATGCATATTGAAGATGATGGAAAAATTAATAAAGAAGGTGGAGCTTACCAAGGTCAATTTTATGAAAAAGCTAATGCTTTAATTATTGAAGAATTAACTAAATCTAAAAACTTACTTTTAGAAAGCTCAATAACTCACTCATATCCACATGACTGACGTACTCATAAACCAATTTTATTCAGAGGAACACCTCAATGATTTGTTTCAATAAATAAAATTAAAGATCAAATTTTAAGCCAACTTGAAAAAGTTAAAACTTATCCAGATTGAGCTAAAAAACGTTTAAGCAACATGATAGTTGATAGAGATGACTGAACTATTAGTCGTCAAAGAACTTGAGGTGTGCCTCTTATCATTTTCTATGATAAAAGTAAAAAACCAGTTTTAAACGAAGACATTTTTGATCACATTATTAAGCTTGTTGAAAAACACGGAACTGATATTTGATATCAAAAAACTGCTGATGAATTATTACCTAAAAAATACCAAAACAAAGGTTATACAAAAGAAAACGACATCATGGATGTTTGATTCGACTCAGGTTCAACATTTATGGCAGTTAATATTGATAAAGGTTTAGAAACTCCTTATGATTTATATCTTGAAGGTAGTGACCAATTTAGAGGCTGATTCAACTCTTCTTTAATTAACTCAGTAGCTTATAATGGTAAAGCACCATACAAAAACATTGTTTCACACGGTTTTGTTTTAGATGCTAAAGGTGAAAAAATGTCTAAATCAAAAGGTAATGTTGTTGATCCTCTTAAAATAATTAGCAAAAATGGAGCTGATATTTTACGTCTTTGAGTAGCCAATTCTGAATACTCAAATGACGTAAGTATTAGTGATAATATCATTAATCAAAACAGCGAAATTTACCGTAAAATTAGAAACACAATTAAATTTATGCTCGGTAATTTAAATGGTTTTAAATATGACTCAAATCTCAAAAGAATTGGCGTACATGCCTATATTAAAGAGCAATTGGAAAAAGTTAAAGCTCAAGTTATTGAAGCTTATGATGAATATAAATTCATTAACGTTATTAAACTTTTAAATAATTATGTAGTTGAATTATCAAGCTTTTATTTAAACATTTCAAAAGATGTTTTATATGTCGAAGCTAAAGATTCAATTAACCGTCTTATGACTTTAACAAATATTTATGAAATTGTTGATTTTCTAATTATTGCTCTTGCTCCAATTCTTCCAACTACAGCTGAAGATGCTTACAAAAACTTTAATAAAGTAAATAAAGCTGAAAGTGTTCATCTCGAAACTTTAGCTAACCCACTCAAAGTTAACGAACAAGTTTTAACTCAATGGCAAGACTTTTTTAAACTTCGTGATGAAGTGAACTTATCACTTGAAAATGCAATTAAAGAAGGTTTAATTAAAAGAACTAATGAAGCTAAAGTTGTAATTAAAAGCGATAGCGACTTTATTAAAAGTCTTGATTTAAGACAATTATTAATGGTTGGTAAAGTTGAATTTGGAGCTCAAACTTCTGTTTCAGTTTTCAAATCAATTAAATGCCAAAGATGTTGAAATCATTTCATGCCTTCACAAATTAAAGAGGAACTTTGTCCTCTTTGTCACAAGGTAGTTTTTAATGAGTAAAGAATACAATGGCTATGAGCCAGAAAAAGAACCATTAAAAAATACAATTAGTAAAAAATGAAATGATTTTATTAACTACTGCAAAAATAACAAAAAGAAAATCTTAATTGCTTTTGGAATATTTTTTGCTGTTGCTTTTGTTGCCATTCTAATTGATCAATTAACTAAAACCTTCTTATTCGAATGAAATGAAGAACATAATGGTGGCAAAATAGGAAAAGTTTATTATGAAGGAACTTTACTCGGAATTAGATCTGTAGGTCACTATGGAGTAACCTTCATTCCAAGTGTTGAAAATAAAAAAGGTGCTTTAATCTTTATTCAATTTTTAAGTATCCTAATTTTACTTAGTTTATTATTAGTACCACTTTTATCAAGACACTTAACCACTATTATTATTTGTGCAATTATTTGAGCCGGTGACTTTGGAAACATGTTAGACCGCTTTATGTTTAATATGATGGTTAAAGATATTTTCTATGTTCCTTTTATGGAAAAGTGAACTGGCAAAGTATTAGGAACCTTTAACTTCGCAGACTTATGTATTGTTTTAGGCTGCATCTCATTAGTACTCTTCTTTGTGATTGAAATGATATTAGATAAAAGAAGAGAAGATACACACCAAGAAGCACAAGAAATAATAGATGCAATTCAAGAAGACAATAGTCAAAATAATACTGTTGAAAATGATCCAAAAGAAGAACAAGAAGACAATAAAAAAGAATAATATATAGGCTTTAACATGATGTTAAGGTCTATTTTTTATGGCGACATTTATACCGACAAATAAACCGACATTTTACTTGGCGGTTTAAATTAAAAAGTATATAATTTTCATAAAGAGGTGAAAAATGAAATTTGTTGAAACAAATAAAATTGAATTAAAGGAAAAATTAAATGATAACTTAATTAGAGAAATTGTTGGCTTTTTAAACACAGAAGGCGGAACAATTTATATAGGTGTAAAAGATAATGGTGAAGTAATTGGCATAAAAGATGTAAATAAAGCTTTTACTGAATTAAACGAAATTATTTGTGATCAAATTGAACCAAAAGCGGAAAAAGAAGTAAAAAGTGAAATAAAATTTGAAAATAACAAAATGATTATGGGAATAAACGTTCAAAAAGGAGAAAAAGCTATTTATTACATAAAAAAATATGGTTGTTCTTCAAAAGGATGTTTAGTAAGAGTTGGGACAAGCACAAGAGAAATGTCAAATGAAGAAATAGAAGAAAGAAACAAAATTAGATTTTATGATGAGGACAACATAATAAATTATGTTAGTGATTTGGATGATTTGAAATTTTCAATATTAAAAATAAATTATGAACTAAATAATTTACATTTAAGAAATAAATTAGGTAGTTACAATAAGCTAGCTGAATTGCTAGCTAATGAAAATAAAATTGACTTGAAATATGTAAAATTTCAAGGAAATGATAAAACAGTCTTTTCAGAAATACAAAAATTCAGTAATAAAAGTATATTGGTTGGTTATTCACAAATTTTAGCAAGATTAGAAGTTGAAAATAAATGCTATGTAGATACTAGTTCAATGCAAAGAATTGAAAAGACTTTATTTGATTTTAATGTAGCTAAAGAGGCTTTATTAAATGCAATTGTACATAATGATTGATTGAGTGGCGTACCAACTATCCAACAATTTGAAAATAGAATTGAAATTATGTCTTATGGTGGATTACCAAAAGGTTTGAAACTTGAAGAATTTTATCAAGGAATAAGTAAGCCAAGAAATCCAAAATTAATGAAAATATTTATAGATCTTAATTTGGCAGAACAAACCGGACATGGCGTTCCACTTATTCTAAAAAAATATGGAAAAATGGCAATTGAAGTAGAAAAAAACTATATCAGGATAACAATACCTTTTAACAAGGAATTGCTACAAAAAAATAACAATTATGAAATTAAAGAAATAAAAGACAAATTAAACAAAATCGAAAATCAAATATTCGAATTAATTCAAAAAAATGAAGAATATACAGCTTATGAATTGTCAAACGAATTAGAAGTTTCTAAAAGAACAATAGAAAGAGCTTTAGCATCTTTAAAAAACAAACTAATTTTAAAAAGAATTGGTTCAAGAAAAACAGGAAAATGATCTGTTTTAGGAATAAAACCAGAAAATTAGAAAAAGGAATACAATTAAATCATGTACAAAAAATATTTATATCCAAAAATTGAACCTTATCAAAAAGGTTATTTAAAAGTAGATGATATTTATCAAATTTATTATGAAGTTAGTGGCAATCCACAAGGTCAACCAATAGTTTATGTTCATGGTGGTCCTGGTGGAGGAACTAGTGAAGTATGTAGAAGATATTTTGACCCAAAATACTATAAAATAGTTTTATTTGACCAAAGAGGTTGCGGAAAAAGTACTCCAAGTTTAGAGTTAAAAAATAATACAACTTGAGATTTAATCAATGATATGGAAGCCATTAGAAAAGAATTAAAAATAGATAAATGAATCCTTTTTGGTGGCTCATGGGGCACTACTTTATCATTATCTTATGCAATTAATCATTCAGATAAAGTTGATAAACTTATTTTAAGAAGTATATTTTTGGGACGTCAAAGTGACATCGACTGATTATATCAAGAAGGAGCTAGCTACTTTAAACCTGAAGCTTATGAAAGATACACATCATTTTTAAGTGATAAAGAAAGAAAAAATATAGTTAATGCTTATTATAAACATATACATTCAACAAATAAAGAATTAAGACATAAAGCATTAATTGAATGAACGAGATGAGAATCATCTTTAGTGATGCTTAAAGAACAACCATTTAAAGAACCTAAAAAACCAAAGTGAATATATGAAATTTCATTAATTGAAAATTATTATTTCTATAATAAATGTTTCTTCGAAGAAAACTATATTTTAAATAATATTGACAAAATTAAAGATATTGAAACTTACATTGTTCATGGTCAATTTGATTTAGACTGTCGTCCAAGTGGCGCTTATGAACTTCATCAAAAACTAAATAATTCACATTTAATTATGGTTGAAAAAGCAGCTCATTCACAAAGAGAAGTTAATATAACTAAGGCTTTAGTTAAAATTACAGATCAAATTAGAAAAAAATAGTTTTATTTAGGACTGCAAAAAATTTTGAATAAAAAAAGCCACATCATAGATGTAGCTAAATATGTATAACCCTTTACTTCCATATTTGAAGTTTTTAAATAATGCTTTAATTTTATGAATAATTTTTACACAAACAATAAACATTAAAAATATATTAATAAAATAATAAGCACTAACTAAAACTATTAAACACTTTCAATAACAATAAATCTAAATTGTAATTTTAAGTATAAATAGCATTATTAAGGAAAGATAAATTATTATATTTCAATTAATGTTTCAATATTAGCTTTATCAATAGCAACTTGCATTGTTGCTATTTCTTGTTTTAATTTTAAATTAGTATTTTTTAATTCGTTAATATCATATAAATATTCAATATATTCAATTTGACCATCGTTGGTAGTTTTTCTTGTTTTTTGCTTTTTAGAACATAACAAATCAAATCTTTGAACTAAAGAAGATTTTTGTGCTAATAAAATTAAAAGTTCAGAAATACTTAATCCTTTATAACTAGTTATTGTTTTGATGTTTGCTTTTTGAACAATAATTTTAAGTCTTAAAATTTCTTGATTAATTTCTTGACGTTTTTTATTAGTTTTATTAAAATTATACGATGATTTTTCAACTGTTTCATCTTTTAAATATATAACATAATTATCTTTATCTTCTTCTTGATTAAGAACAACTAATTCGTCCTCAAGAATTTTAATTCTCTTGATAATTTCTTTGATCGATAGTTTCACATTTGCTCCTTTATATATTATTATGATATAACATTTTCTATATTATGCAAACAAAATGAATTAGGTTATTAAAGTTGAAAGTTATAAAAAAAGTAACATTTTTTTGCTAATCTGCGATAATTTAAGCATGACAACAAATACTAATAAAAATTCTTTAACAACAACCATTCAATTAATATTAGAAGATGGGACATTAGACGGGATTTTGTATCTCAAAACTTTAAGATGGCCTCTAGGAGGAATTATGTTGGTAAGTCCTCGCGATAAGGTTACTAAATTATTGGACGAATATGAAGAATGTAATAATTGAGGAATATATTTATTGATTGCAAAAAATCAAGTTTATGTAGGACAATCTTCAGAATTGAAACAAAGAATTGAACAACATTTATTAGGAAAAGATTGATGAGAAAAAGTTTTCTTATTTACTACATCAAATAATTCATTAGACAAAAGCGGAATCGATTATTTAGAATCAAAACTAATTGATAAGGCAAAAGCGAATAATAAATTAAGTTCAGATAATAAAAAAAATGGAAACAAAACTAATATTGATAGATTCCGAGAAACAGAATTAAAAGAATATTTAAATGAATTAATTTTCTTATTAGACTTTATTAATATTGATGTTTTTAACGATAATACAAACAAGAAAAATAATTTAGAAAATGTGAATTTATCAAATATTATTAAACCCAAATCAGAAGAAGAAATTAGAAGTCGAAGTAAACAAGAAATATTTTCTTATATTGAAACAAAAACAAAAATTGATTTAAAAAATAATACTTACTATAGTAAATATTATGATCATAAAAATTGTTATTGGTTTACTTTAAAAACTAATTCAGTTAATAGTGATTTAAAATTAGTACTAAATGATATAAATTCTCAAGAAATAACAATTTTGCAAATTCCAGCCAAAAAATTTGAAATTTCAGATACTAAAGGAACGAACAAATTTCAAAAAACTAGAAGCGATAATAAATTTGATTTATATATAAATAATGTTTTATTCGAAAAACAAAATAAAATTGACTTTACAGAATATGTAATAGATAAAATTCATTATTCTTCCTCATAAGAATACATTTGAACCACTCTATGTGCTTTTTTTTAATAAAAAAGTGTCCAACTAAGAACACTTAATTTACTCTAAAGAATCTAAGTCAACTTTAGTAACTTCAATTCCTTCAACAATTGTTTTTTGATTTTGAATATCAGAATTGTTTTTATCTTTATCGCTAAATATTTTATGTAAACCAACAACATATTTATAGAAAACATAATTAATAATTACTGTTGCAAAGATTAAGAATAAGATAGCTAAAATAATAAAAGCAAAATGAACATAAACATTTAAATCCTTAGTAAATACCACTTGTGCATATTTGAATAAAGCTGTTGAACTTATTGCGATAGGGAATGTAAAACAAGCTCAAGTGAAATAAAATTTTTTAGATTTTATTGAGATAGCTAAAGAATAAAAATATGCAGCTCAACCAACTAAAATAAATAAGAATAATATTCCTGTAAAAGTATATAAAATTACATAATTATTTAAAATATTTGCGTTTGGATTCTTAGGATCAAATAAAGTTAAAAATGCAAAACTTAATACCATTGCTGGAGCACAAAAAATTGCTAAGGAAGGAATATCTTGATGATTTTTGTGGCCTACAAAAGCAACCTTAAATGCTACTAAAAAAAGGTTAAAAAGCAGAATAATAAACCTATAAATCAGAGTATTTGGTAAAAAAGCAAAGGTATTATATTTTGATAATTATTAGCTCCAAAAGTAAGGATTATAAAACCAACTGTTGGAATAAATCAAGAAGCAAAAGCTTCATCATATTTAAAATCATGTCGAACTATAATTAATACTATAAAAGTTATTAAATAAGCTATTTGTAATATAAAAGCAATGAATGAAAATACCAATGGAATATATAACCATTTTTTAGCTTCACTAGCTTCTAAATAAGTATTTAATATTGAGGCTATAAAATTAAAAATAGAAATAAATGTCATTAATAAAGTAGGTATAAATGCTGCTGTATTTGGAGCTTTTGCTTCTTTATTAAATACATGTCTTGCTTTAATAAATCTTAAGCAAACAATAAAAGCTAAAGCAAAAGCAATTAACATCCAAAAAGCCTGAATGCCAATTGAAACATTATTAATTAATTCTTCATTGCTTGAGTTTTTAAACTCATTAAAAGCTCCATTATAAGCACCAGCTAAACCACTCATACCAAGTGCAAGTCCTCCTAAACCAAGAGGCAATTTTTCTAATTTATTTTTAAAAGTCATGAATTAATTTTATAACAAAAGTTTAATTTAAGAATAAACATAATTAAAATCCGCGTTATAATCTTTTTTAATGAAAAAATATGTAAAATTATTTGAATTTTTTGCTGGTATTGGAAGTCAATATCAAGCATTGAAAAATCTAGAAAATGAAATGAATTTTAAAACAATTTCACTTGGCACATGTGATTTTTTTATTCCATCAATAATTAGTTACATGATTATTCATCATGGACTTTTAAAACCAGAAAATGAATTGTCCAAAACTGAAATGATTAAAATTTTAAAACAATTTTCATGAAGCAAAGACAGTAAAAAGTTAGTTGCAGATAATTATTTTGAAAAAATGAGTGAAAGCAAACTTAAAAGCATTTTTCCTTATCTATTTGCATATGTTGACAATGAATACTTTGCTAAAAAATATCAATTTTCAATAGATCAGAGAGAGAGAGAGAGAGAGAGAACTATCGCTTAAGTGGTACTGACATTAATAAATATAATGTTCTACCAAAAGATATTGATATAATGACATATTCTTTCCCTTGTCAAGATTTGTCGCAACAAGGAAAACAACGAGGAATACATGCAAACACAAGAAGTGGTTTGCTTTTTCAAATTGAAAGAATACTTAAAAATAATACAGATAAATTACCCAAAATTTTACTTTTAGAAAATGTAAAAGCTTTAGTATCTAATAAATTTATAGACAGTTTACATAGCTGAATTAAAACACTAGAAATTCTTGGATATCACTCATATTACAAAGTGGTTAATGCTACTGAATATGGTAGCTGTCAAAATCGCGAAAGAGTTTTTATGGTTTCAATTTTAAACAATAATAAACCATTTGTTTTCCCTAAAAAGTCTATAAAACAGAAAGATTTATCAAAAATTATACTGCCAGAAAATGAAGGAAAAAGTTTTCAACATTTGCTCAAATATGAAAAATTACCTTTTAATAAAACCAAAAGTCAAATTACGAAAAGTAAATTGTTAAATTACACATCGTTCAATTCTGAAGCTTATATTTATGTACCTATTGGTTTTGGCCCTACATTAACAGCTTCTGGAGCAAATAGCCGTTTGAAATTTTATTTTGAAAAAGAAAATATATTAAGAAGCATTAACGCGATTGAAACTTATCAATATATGGGCTTTGAAATTAAGAATGCTAAAGATATTATTAAATCAAAATTAGTTACAGAAAATGAAATGATTTTTACTTGTGGAAATAGCATAAGTGTTGAAGCGCTTGAAGCAATTTTTAGAGAGGCTATAAATGAGTACTTATAAAGAAAAATCTGATTACATTTTAAGCGAATATAAAATATATAAAATACCTTTTACCAGCAAAAATTATTTAAAAAAGGAATCTAAAAATAAATGATTTTTTAAAGGCAATATATATGTTTTTATTGGTATAGATAATCTTGTATACAACTGAATTGTAAAATTTGATGAAATTTCATTGGATTGTTTTTATACACCAATATGAAAAGAAAATAAAAATCACAATTATTTGATAAGAGAAAAACTTTTTAAAAGATTGAATATTGAACAATTGCCAAGACCATCTATTAAAGACAATATGATAAAACATTTAAACAAAGAAGAAGTTAATATTGTAAATAATTTTATTAATGAAAATTATGATTCTACATTAAAGAACTTAACTGCTTTAATAAAGGGAAATAATCCAACTGGTAGTTTATTAAAACCAGAAGCATCTGAAATAATAGCATTTAATAATGAAAAAAGAATTTATAACTTAATTATGTTTCTAGACAGAATATCATTAATAAAAAAGAGAAAAGATTTAATGACATTAAAAGATATTTTTAACATAGAACAAGAAGATTTTATAAATGCTTTATATAATGCTGAAAATAAAAACATTATTTTAAAAGTAGAAGACTGTATTAATTTATACACAAAAGATGCTGATGACTATTTCAAAAATTATTTTGTTAAATTAAATAATTCTGATTATTATGAAGGCAAAAGAATAACAAATTCACAAAGCATTAAAAGAGCAAAAGAAATCATTAATAAATTTGCTAAGAAAATTTCACTTACATATAAAATTGACCAAATTTGTAAAGAAGAAGAAGGAAAGTACCACAAACATAAGATAGATTTTAATGCAACTTTTAATAATTGCTGAGATAAAATGGAAGAAGCTGCTCATATATATAATAGAAAAGATATCAAAAAGGAAATGGTCAAAGAAGCTATTAAATATGATGTTTTTTCAGATGACAAAAACAGTGACAAACACTTAAAAATAATTTTGAATAATATAAAGATAATTGGAAAACAAATCAGTGATCCAAATAATTTACTAAACTTAGATAAAACAATGCATGGATATTTTGATGACAACTATTTTACGTATGATGAAAATGGAAAATTAATATCTTTAAAAGATAATATTAAATTTGAAAATTTTTTAAATAAATTTGATTATTACAAACAAATACCACTGCAAAAATTAAATGAAAACAGAAAAAAATACATTAAATTAAGAAACGAAAACATAAAAAATAAATAAAAAAGGTACATTGATTTTTACCAAAGTGCCTTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAATTGTGAACTGTCAAATTAGCAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTATAGGCAAGAGTAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTGAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAAAAGGTACATTGATTTTTACCAAAGTGCCTTTTTTATTATTAAATTAGTAAAATTATTTCTTGTTTTCTTCTAAGTAAAGTTTAAGTGAAGCAGCTCTTGCAGCTTCTCAAATAATTTCCGAATAAGTTGGGTGTGGGTGAATTGTGTAAGCTAATTCTTTGACACTAACTTCTTGTTCCATAGCAAGTGCTATTTCCGAAATATAGTCAGTTGCATGAGCTCCAATAATGTGAGCTCCTAATATTTGCCCATATTCACGATCAACAACTAATTTAACAAAACCAGAAGTTTGTTTGGTTGCAATAGCTTTTCCTAAATAAGAGAACATGTATTTTGAAGTAAAAATATTGTATCCTTTATTTTTTGCTTCTTCTTCAGTTAAACCAATGAAAGCAATTTCAGGGTTTGTATAAATACATCCTGGAACTGGTTTATTGTGATAACGAACATTTTTTTCACCTAAAATGTTACCAACAGCAATTAAAGCATGTTGATATGCAACGTGAGCTAACATGTTTTGTCCAGTTAAATCTCCAATTGCATAAACGCCTTTAACATTTGTTCTCATTTTTTCATCAACTAGAACTTCCTCTCTAACTCCGACATTAACTCCAACTTCTGCAATACCTTTACTTGATGGAATACGTCCAACAGCAATCAAATAAACATCAGCTTTAATTTTTCTTTCTTTACCACCAATTTCGTAAAGAAGTTCTTTTTTATTATTTAAACCTGTTGAAGTTGCATTGTAAATAATTTGAACCCCATGCTTTTCTAGATGTTTAAGAATTTCATTTGTTACATCATGATCAATACCAGGAAGTAAGTGGTTTTGGTTTTGAATAATAGTTACTTTAGCACCTGCTGAAGCAAATACTTGAGCAAATTCAACGCCAACAACACCACCACCAACAATAACTACCGATCCAGGCAATTTTGAATCATAGTTAATTGCTTCGCGGCTTGAAAGAACATAATTATTTTCATATCCAACCTTAAATCCAGGTAAAGCACGCAATCTACGTGAGTGACTGCCTGTGGCTAAAATAATATTTGTGCCACGATATACTTTACCATTTATTTTAATTTCGTGAGCTCCAACAAATTCTGCTTCGCCTTCTTCTATTTTACATTTAGAAGCAATCATTAATTTTTTAACACTACTTGAAATTTGATCTACTACGCCTTTTTTACGTTCATGCATTTTGACTCATGTTTTTTGTAGATCTATCTTTAATTTATCTAAGTTCCCAACAATACCATAACCAGCAGCATCGGTTAAGGTTTCTAAAACATCAGTTGATTTAAGCATTGCTTTAGTAGGAATACATCCTATATTAAGACATACACCACCTCAGAATTCTTTTTCAACAATTAATACTTTTTTGCCTGCTTTGCCTAATTCTTCAGCGGCTAAATATCCTCCAGGACCACTACCTACAATAATTACATCAAATTCTTCATCAACTTTGCCTTCGTATTTTTTACCTTCAGAAGTAAATGAAACACCATTGCTTTCTTCTTCTTGGTTAGCTTCATAAGTTGAAGCTTGAGGTGCAACTGGTTGAGGAGTTTCTTTTGCGGGTGAATTTAGTGAACTGAAATCTAGATTTATTAATTCACTTGAATTTGGAGCTTCACCTACTAAACATTTTTCTTCTTCTTTATTGGAACTTGGTGCAGATGAAACTGAAGAAGAAGTAGGATTTTTACTTCCTGCATCAATAACAATAACTTCTTGTCCTACATGAATTACATCACCTGTTTTCATTTTAATTTCTTTGATTATTCCATCTACTGGAGAAGGAATTTCAGAAGCAACTTTATCAGTTTCTACTGAGAATAATGTGTCGCCTTCTTTTACTTTATCACCTATTTTAATAGGAATTTCACCAACTGTTCCTTCGTGTAAACCTTCACCAATGTCAGCAAATTTAAAAACATAAAAGCTTGTAGATGATGAAGTAGCAGGTGTACTTTTCGATTCTTCAACTTTAGTAGCAGGAGCTGCAGCTACTGAGCCACCTTTGCCATCATCAATTACAAATATTTCTTGGCCAACGTGAATTACATCACCAGTCTTCATTCTAATTTCTTTGATTATTCCATCTACTGGAGAAGGAATTTCAGAAGCAACTTTATCAGTTTCTACTGAAAAAAGTGTATCTCCTTCTTTAATCTTGTCGCCAACTTTAACACTGATTTCACCAACTGTTCCTTCGTGTAATCCTTCACCTATATCAGCAAATTTAAATACGTACATATTAATACACTCCTAAAACATCTGGTTTTTCTAATAATTCTTTGATTCTTGAAGCGAATCTTCCAATTACGGCACCATCAATTCAACGGTGATCAGCAGCGACAGTTAGGTACATAACTTTTCCAGGTACAACTTGTCCATTTTTAACTACAGGCTTGTCAATAATAGCACCAACACCACCAATAGCTAATTCTGGATAGTTAATAACAGGAACTCCAAAAAGTGATCCAACTGAACCATAGTTAGTAATAGTAAAACCGGCACCTTTCATTTCAGCAGGTTTAATTGTTCTATTTCTTGCAGCGCCTGCAAGTCTAGTTACTTCGTTAGCAATATTAACAATACTTAAATTATCAGCATTATTAATAACAGGAACCATTAAACCAGCTTCAGTATCAACAGCAATACCAACATTAATTACACCAGGGTAATCTAGAGTTTGATTTGCTTCATTATATTTAGCAGTGAATACTGGAAAATCTCTTAAAGCAATAGCAGCTGCTTTAATAATATAAGGCAAGAATGTAATTTTTACATTATCACTTTTAAGTACAATATCTTTGATTCTTGAACGTAAGTCTCAAAGAGCAGTCATATCAATTTCGTGAACTAAATTTGTATAAGCAACATTACTTCATGAGTTAGTCATAGCTTTAGAAATTGCTTTTCTAATTGGAGTAATAGCTTCTGAGTGAGCTGAACGATCAATGGCAGGACGAGGTGCTGGAGTAGCTTTAGGAGCTACTGCACTAGGTTGAGTAATTACTTTATTATCAGCTACTGGAGCTGGTGCAAAGTTTGAATGAGCAGCACATGAAATAGGACTGTTTTTAAATTTTTGAATATCTTCTATAAGAATACGGCCATCAATTCCTGAACCTGGAACTAAGTTAATATCAATTCCAAGTTTAGCAGCCATAGCTCTTGCAATAGGGGTTGATTTAATTTTCATTATTTCTCCTTATAAGACTATAAAACAAGTCTTTTAGTAAATTAAATGGTCTTTAAATATTAAAATCTAAATTCCATAACTTCTTTTATTTTTGCAAGAATTTTGTCTTCATTGATGCACATTCATGCTTCGCCTTTTGCAAGAGGAACAGTTACATCATAACCTGTACATCTTGTCATAGGTGCTTTAAGATATTCAAATGCTTTTTCATTAACTCTAGCCATAATTTCAGAAGAAACTGAAAATGATTTAACAGCTTCATGAACTACTAATAAACGTCCTGTTTTCTTAACACTTTCAACAATAGTTTTAGTATCAAGAGGTTTAATTGTTCTTAAATCAATTAATTCAATTGAAGCGTTAGGATTGATTTCTTTATATTTTTTAACAGCATTAATTGAGTCGAATACTTGAGCTCCATAAGTTACTAAAGTTAAGTCATTACCTTGAGTTAAGACATTAGCTTTTCCAATTTCAACTGTATATTCGCCAGCTGGAACTTCTTGTTTCCCAGCACGATAAATTTTCTTATTTTCTAAGAATACTACTGGATCTGGATCATTTAAAGCTGCTAATAATAAACCTTTAACATCATAAGGAAAGGCCGGCATAACAACTTTAACACCTGGAATGTGAGCATAAATAGCTTCTAAAGCTTCTGAGTGATGTTCCATAGCTTTAATTCCACCACCCATAGGCATTCTTAAAATCATAGGCACGGTGAAACGTCCTCTTGAACGGTTTCTTCATCTAGCTGCATGAGTAAATAATTGTTGCATAGCTGGATAACTAAAACCTTGGAATTGAATTTCGGCAATAGGTCTAAGACCTGCTACTGCAGCACCAATTGCGGTACCAGCAATTGTTGCTTCTGAAATAGGGGTATCAAATACTCTACTTTTACCATATTTTTTTTGTAAGCCTTCTGTAGCTCTGAAAACTCCACCTTCAACACCAGCATCTTCACCATAACAAACTACACGGGGGTCTTTAGCCATAGCAATGTCTAAAGCATTATTAACTGCTTGAACATTATTTAAAGTTAATTTTTCTTCAGCCATGATTATTTGCCTCCTTTAGCTTGTCAGAATTTAATAGCTTCTTCTCTTTGCTCTAATAAATCATCATCAAGCTTTTCATATGTATAGTCAAAAATATCTTCTAATTTGGTATTGATGTCTTTCAAGCTTTCTTCATAAGTTTTCTTAACTTGTTCAAGAGCATCGGCTCAAATCTTTTCTTTTTCTTTTTCAGTAATGATTTTTTTGTCTTTCATATATCTTTCGATGCGATGCATTGGTTCTCATTTTTCATTTTCTTTTTCGTCTTTTTCACTACGATAAAGTTTTGGATTGTCACTAGTTGTGTGAGGGCCTTGACGTCATGTTAAGAATTCAACAATAACTGGTTTGCTTTCTTTTCTAGCATAATCAATTGCTTCTTTAATTACTTCATAAGAAGCTAGAAGGTCATTACCGTCAACTCTAACACCAGCACAACCAAAAGCATGAGCTTTAGCTGCAATAGTAGCTGATCTACTTTCTTCTGAGTTAGGTGTTGAAATAGCTCATTGGTTATTGTTTACACAAAATACTGCGGGTCATTCTTGAACAGTTGCAATATTAACACCTTCGCTAAATTCTCCTTCAGTAGTTCCGCCATTTCCAATAAATGAAATAGCTACACCACCAGTTTTAAGTTGTTTTTGTGCATAAGCAATACCAGCACATTGTGAAATTTGAGTTGCAATAACAATATTAACCGGCATTACATTAACATCTTTAGGATGGTTGCATCCTCATTCTTGACCATTTCAGTAACGAATTAAGTCAATCATAGGAACGCCTAAACGAAGCATAGCAGCATTAGATCTAAAAGCTGGAACAAATCAGTCTTTCTTTTCCATAGCCATTGCTGTTGCAATTTGTAATGCTTCTTCACCTAAGTTAGGTGGAAATGTAAGCATACGTCCTTGACGTTGCAATTGCAACATGTATGTATCTTGTTGTCTAGATAGTACCATTCATTTGTAAGCTTCTAAAACTTCAGCATTACTTAGTGGTGCTTTGTAATTTTTTTCAATTACTTTACCGTCTATGTCTAAATGTCTAATAATCTTATTTGGATCGTCCATAATTTTATTAACATCGACATATTTGTACTTTGTTTTCATAAAGATACTTCCTTTCTTGAGTCAAAATTAAGTCTTTTTATAATTAAGACTATAAAAATTTTATTATTTATATGAAAATATTTCCAAAAATATATTTATATAGCATTCTTTTTGTATCTTTTATGGTAATTTTTTCATTTATTTTTCTTATTCTTGATTTTTTGAAAACTTTTTTGTTAATTTTGAAGCAAAAAGTGAAATTTATTTTTTTCATTTTAAACGCCAAAAATGATATAATGGCTTATAAAAGGTAGCAAAAAAATGAAAAATATTGATTATAAAAGAATTTTTAATAAAGGTATCCCCACAAATATTATTAACTTGTTGACTGAAATATATGAATATAAAGGAAGACAAAACATTTATATCAATAACAAGAATTTAGAAATCCAAAAGCTTAAAAAACAAGCTTTAATTGACAGTGCAGTTTATTCAAATAAAATAGAAAACATCAATATAAGCAAAAAAAGAATTAAAGATATTTTGCTTGAAAATGATGAGTTGCAAAATGAAAATGAAGAAGATATTAAAGGTTATTTTGAATTATTAAATTCTCTATATAATCAAGAAACATTTGAATTATTAAACACTAATTTGATTCTTGATCTTCATTATCAATTGTTTCAAAACTCAAATACCAAAATGAGTGGGCGTTTTAAGTTAAATGATAATTTTATTTTAGAAACAACCTTAGAAGGTACTCAAGAAATAAAATTTATTCCTGTCAAAGCATTTTTTATTAGTGAATATATAAATAATTTATGTAATAAATATAATGAAGAATATAAAAGTAAGAAGATTAATTCATTGCTTCTAACAACAGCTTTTGTTTTGGATTTTATTTGCATTTCGCCTTTTAATCACGGCAATAGCAGAATGTCTAGAATTCTTTTAAATTGACTATTAAGTGTTGGTGATATTAAAGTCGTTCAGTATATAAGTTTAGAAAAAATCATTTTTGAAAACTTGGAAAAATATCAAAATGTTCAACAAAAAAGTTCATATAAATGACATGATAATTTGAATAACTATTATTACTTTATAGAGTTTATTTTAAAAATTGTTTTACAAGCTTATAAAGAATTTGAAAGCCGTTTTACAAGTTTAGAAAATAAAAAAATTGATGTAGCGGACAACATTTTAAAACTGATAGCTCAATATCAAAAAGTAAGTAAAAATGAGCTAATTAATTTACTACCTGAAAGCAGTAAAAGAACAATTGAAAGAAAACTAAATGTCTTATTAAAAGCTAAAAAAATACTTAAAATTAATCAAGGCAAAAATACTAAATACACTATAAAACAGCATTAAAAGAAAAAATGGGACTGTAAAAAATTTTGTGTAAAGTTCTAAAATCTTTTCTTGATAGACAATAAATTATCAAGAAAGGATTTTTTATTTATGAAAAGAAAAACAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTTAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAAAGATGTAAGTGATTTTTGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATGGTGAAGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGAAATTGATCTGAAATTTCTACAATGTTTGATTATTCTCCTGAAATAAGAACGCTTATTTATACTACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAACATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATAATTGATTTTGAAAATGAAAAATAATTTTTTCAAATTTTAAAAGCTTTGCTTCTTTGGCTTCAAAGAAGGCCTCTCCCTTAAATGAGTTAAGGGAGACGGGAGAGGGATTAAGATTTCTTGCATGGCAAGAACTTTTTCCTATTTTAAAATTAAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTGCAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTCTATTGAAAAAAGGAGAGACTTTACACAAAATTCTAAACAGTCTCGAAAAAATAGAGGTTTTATTTAATTAACCTTTATTTTTTCTATTTCTTCATTTAAAACTATAACTACTTGCTTTTTTAACTCTAAAATCACGATAAATTGATTTGTCTTTTTTAGCTTGATTGATGTAATAATCAATTACATCTGGATTAAAGAAATACTCACCATTATTATGTTTATTAACTATTTCCTCTAAATTATCGTAAATCATATTTATCAGTTTTTTAGGATATTTGAGGCGTCTAGAATTGTAACGAAATTCTTCTTTATCTACAATTGTAAAATCTTTTTTAGGATAGCTTTTAACATCTAAATCAAAATCAACAAATTTGATTGTATTATCTTCATAATATGGTTTGGAAGCTAAATTTACATAGACATAATTACTTTTTGGTTTTAATAAAATCAAGGCATTATACATACTATTTTTAGGCATAAATCATAAAACATATTCGCGATATACTCAACCTTTTTTATCAGTTTCAGCTACTTTAGTTTTATACATAAATAGAACATAATGCTGCGGTGTATTTCTTAAAACCTTAACACCATTTCATTGGCGGTATAATTCACCATTAAATTTAAAAGCTTGGACATTAATAATTGATCCAATTAAGGGAAAAACTATTTTTTTAGGTTTTAAATTTTCTTCTTTAATAAGAAGATTATCCTTATTAACTAAAGACATTCATTTCCCTCCTTTTAAAATATATTCCATATTTTTTATTCGATTGTTTTTTGCTTATAAAGCTAGCTTCTATTTTATATAAATTCGTTGAATCTAATTCACTAGGTGCATCACTACTAGTTAAATATCTATCTGGCAATGGATCTTTTTCATATTCTTTGATTAATTCATTATCTTGCATTGCACCATCATTATTTAGATCTTGTCAAAATTCAATAGGTGAATTTTCTTTTATATTTCTATAATCAAAATAATCACTTGTATAAGCTGCTTGTTTTGAAATTAAATCACTTAAAACCATATTTGGAGATAAGGTAAAAGTAGTATCTAATAAATTATCTTTTCCCTCAAATTTAAATGCATTTAAATAATTTATTTTAACAAACTTTTTTTCATCTTTATTATTTGTGAAATAAAATCCTTTGATATTTTTGGTATCTAAATAACCACTAATTCTTGTTTGAAAAATATTATTTTTATCAATTTCAACTGAATAACTATCAGTGTCTCCAGCAATTAATTTTTTAACATCTATTTTGCTTTTAACTTGAGCTATAGTTTTACCATAACCCATTGTTTCAAGAAACAATTTGTAAAATAGTTGTTGATTTTCACTTTTATAAATATTGTTACTATAAAAGTTTCGATCAGCTTCTAATTGAGCTTTATGCATTGTTCTTGATCAATCAATTGAATAACTATTAGCATAGGTCAACAAATTATTTGAATCTTTAAAAGTAACACCATAAAAAGATTCAATTATATCTAAATCATCTATAGTATCTGATTTAATTATTTTTTCATATAAATAATTATAAGGATTTGATGGATCATTAAATGGTATGTAAGCATATTTTTGTCATTCTCTTGGCACTAGTTCAACTAGAGAATAATAATAAGGCAAACTATTTATTGAATAATCGGCTATTAAATATTTTGAATCGAAATCTTTAGTTTCTCTAAAAGTTACTTTGTCATTTTCATCTTTAATAGATTTCAATTTATTTAAAGCTTCTTTGAATTTTTTATTATCGTTTTCATTTGATAAACTTCACAAGTCATTAAGACTTAGTTTATAAGGAACAAATGAATTGTCTCCTGGAAACCATGGATTTATTGAGTTTATTCATGTATCATCATAATTTAATAATTCCTTAAATTGATTAGCAAATTTTTTATATCAAAAATCACTTTTTACTGAAAATTCTAAATTTGAACGATAAGGAATTGTAATTGCTTTATTATTTTTAACATCTTCAATTGAACCATATGAAGCATAACTGTGTGCTCAATGGTGCATGTATTCATGAAAAAGTGAATAAAGTATTTGTTTTACTTTGACACTTAATTTTGCTCCTTTTTCACTTAGTGCCGCACCATTTAAATAAATTCTTTGGATTGTTGGCAAATAAATACCTGCAGATTCTTTAGTGATAATACTAAAGTCATTAACGTTTATAGAATCTAAAAATTTTATTTCCGGTCCATAAGTCATTCTTGAGTAGAAATAATTAGCTAATAAAGCTAAACCATAAGGGCCTAAAAGGAAGCGTGGTTTTTTGTTTTTATCAACGCCATAAGATTTGTCAATAAATTTGACATTGTTAATAGGATCAATATATACTCAACTGTTATTTTCAAATTTAAACGTTCCATTTTTAACTCAATTTCTCTTATCATATTTATAGCTATGTTTTTTCGTATCATACTTTAAAACAGTGTTATTAGCCAAAATATAATCATTAAATTTTAAATCAATAAATTGATTTTTTAAATCATTTGTAACATTGTCAAAATTATTTAATGAAGAAGGACTTTTTGCATATTGTTCAATATATTTATCTTGATTTATAAGTCCTATTTTATCTTCTTTACTTTGTATTTTTATTTTAACTAAATTATTATTTTTATTTATTGTTTCATTTTTATTATTTAAAAGTAAATAAACAGCAACAAACAATGAAACAAAACACAAAAAGATTGACAATGATATTAATGTTCAAAATAATGCATTGTTGCGCTTTTTTGATCCTGATCGCTTAATATTTTGAGGTGGGATCATGATAACCTTTCATAAAAAACTTACATATTAGTATATTTAACTAACATTTTTATTATAAATGATTAAATACCATATTTTTATAATTTTTGAAAATAATATTCAACAATTTAACCAAAAGTAATAAAAATTCCATAAATAAGTGTTAAATTTTGCACAAACTAATTTTTGATACTTCATACTTGATATAATAAAAATAGGTGGTTACAATAAACCACAAAAGGAGATTTATGTATAAATTTAAAGCAAAATTAGTTTCAACTCAAGAAGTTGTTGCACAAGCAAACTCACTTGAAGAAATCGAAGGATTAATCCTTGGCTTCCGTCGTAAACAAAAATATGACGAACACACAAGAGCCAACGAAAAAATCCAAATTATACATGTTGAAAGGGATTCTTTAAAAGGTAAACATAAATCAAAAGAAGAAATTCTTAAAGTTGTTTAATTACAATTAAATATAGGGGCTAAAGCTCCTTTTTTATGTGTCATTTTTTTCATAAAAGCACTTTTGAAGTCTTATTTTAATATAATTGTAATGTTAATATTGAGCTTGGTTGCTTATTTTTTTAGCCTTTTTTCAATATTAATTAAAAGAATAAAAAAATAGAGGAGAACTAATGAAGAAAAGATTTTTATTAGGTGCTTTAGCTCCAGTTGTTGCCTTAAGCCCTATTGCTATTGCAGTTAGTTGTGGAAAACAACCAACCATTGAAGAACAATTCGCTAAATTAAATAATGAAGGAAAATTAGCCTTTATTAAAGCCCTTGACTACAAAGGAATTATGTCAATGGACGAAAAAGAAAAATTAATTTCTAAATTAAATAAAGATGCTGGTCAATTTGGATCAATTGTTTGATACATTAAATCAGTTGAAGCAAGAATTGCAAAAGAAGCTGAATATTCTAGAGCAAAAGCTGTTTTTGACAACTTAATGAAACGTGCTGCAACCGATGGTTTTGAATATGGTCAAAACTTTAATACAACTAAACAAGTAAATAATGCTGCAACAGATAAATACATTCCAGTTATCTTTATGGACATTGATGAAACTGTTTTACAAAATGATTACACAGAAACATTTGGTATGTTAAATGGTGGTTACAATGGAGACATGAAAGAAAAGAACGACCTCAAAGGAAATAGATTTGCTGTGCCAGGTGCTATTGATTTCATTAACTATGTACAATCAAAAGGTGCTTTAGTTGTTTATAACTCAGATATGAACCAATCTACAGCAGTTAGAGAAGCTGTTAAAAAGAATTTGATAAACTTAGGTGTTAAATATGTTTCAGACTTCCAATTCTGAATGCGTGGTTCAATGCCTTATGTAGCAATGGATGGAACTAAAGCTATTACAGACGAAATGACAAAAGATATGTCTAAAAACGATCTTAAAAGTCTAGCTGGAAGAATGAAATTTACAAAAACTTTTGAAACTACACCATGAAGAACATGAACAAATTCAAATGCAGCTTATGTATTAGGTAAAAAAGTTTACAAAACAGACCGTATGAATGGTTTAGACGACAACGCAACAGGATGAAACTTAAAAACTGTTGATTCTAAATCAGGTGAAGCCGTTAGATTGAGAACACTTATGAGAATAGGCGACAACTTTAATGACTTCTTTGATAGAAACTCAAAAGGTAAAGACAATGACAAAAGAACTCAACAATACGTTAAAAACACAGACATGAGAAAATTATTCACAGATATTAATGGTGCTCCAGGATTAAGATTTGTTAAAAAAACAGATGGTAAATATGGAGAATTTCAAAAAACAACATACTGACAAGGTTACAGTATGGTTCCAGGAAATAGTGAATATGGTGGATGATTAGATTCATTCGGATACAGTGAAACTTACAAAAACATCTATGATGAATTGAAGAAAATATTAGCTAATCCTAAATATCAAGCTGAACGTACAACTGCAGATATTTACAACGAAAACTAATAAATCAATATTAATATCAGGCTTATTATTGAGCTTGATATTTTTTTACTTTGCAAAAATAAAAGCACCCTATTGAAAGGATGCTTGGTCAATTACTTGAACTGGTACGTCCTAGAGGACTCGAACCTCCGACCCAATGGTTAAAAGCCATTTGCTCTACCGCCTGAGCTAAGGACGCATATTTGATGCCTAACGAAGTAGACAACTGGTACCCAGAACTGGACTTGAACCAGCACGAACTAACGTTCGAGGGATTTTAAGTCCCTTGCGTCTACCTATTCCGCCACCTGGGCATTTGCTAAAATGCTCTTTAATTATAAAGCAAAATAAAATAAAAACAAATATTTTTTAAAAATAAAATTTTGTGTTTTCATCTATATTTTTTGCTTTTAAAATTATAAAAAAGGAGTAAATTATGTTTATAAGTTATATTCTTAATAAAAGAGGTTTAAAATGAAAAAAAATAAAATTTTTAATAGTTTATTATTAACTCCATTAATTATAACTCCTATAACATCTTGTGGTAGTAAGCCAGAAGAAAAAAAGTCATTACCATTACCCAGATCTTGCACAGATTATGACAAAAAAGAATTGAATAAAATAGTTGAAAAAAATACAAAATGAATTGAAAACTCCAAAACGCATGAATTAGTAAAATATATTGATTACGATTTTTTCTATGATGTTGTAAAAAAATGATATTGTGATGATAATAATAATTTAAAATCTGATATTTTTTATGTTCCAGCAATAAATAAGATATCAGTCCTTAAGGAAAAAAATAATATTGATTTAGTTTCAAGTTATATATTTTCAACAAAAAAAGATCTTCAAGAAGAACTGGACTGAATTAACAATAACAAAAAAAAGGCAATTCAAAAATATTCTAAAAGAAATGAAACACTAAGTGATGAAGAAAACCTGAAGAAAGTCAAAAAAGAAATAGAAAATGAAATTAAAATAAGAGAAAACAAAAGGGACATTTTTTCTTTTAAAAAATTTGTTATTTCATCTAACTTTTTTACTTTAGAAACTAATGATTTACAAGATCATAAATTTAAAACAAGCATGTTAAGACTTTCTTTATTAAAAAACTGTAATATTGATTTTGACTACAGTGTCAACGATATATTTATCAAACGAATTAATATAAAATTAACAGAATTCGACAATGTTCTAATTAACTCCTTTAGTGACCCAAAATATTCTAGTTATTACACAAATGAAATTTATTTCTTTTTAAATAATTTTAATAGTGATAAACAAAATATCATTGTTGATAAAAATAATTTTGATTATTATAGAGCAAATCAATTTCAAAATGTAAAAATAAACACTTTAACATTAAACAATTTTGAACCTGATTCGGTTGAAAATGCAAATGTTATAGCAACCGATATAGATGTTAATACTATAGAATTTAGTGATGAATTTTCAAATAATATTGATATAGATGAAACATTTAAAAATATAAATTTCAAAGATATAAAAGGTGTTCAAAATGTGTTAAAAAATTTGAATTGTGTAAGTTCAAAAGATAAATACATTTTATTTGAAAAATTAACTAAAATAAATAAGTTGCCCGCTTCATTTAAAATTAATTTAAAAAAGATACATTATACAATAATGATTTATCAGTAGGTGATTTAAACAGCATTTTAAATGCATGTAAAGACACTCTTGACTTATCAGACTCAGATTCAATTGACTATTTATTGTTTGATGACGAAAGACAGGATTTTTTTCGCACTAAAAAGATTATTTTTCCTAAAAAAATAAAAAGAATTGAAGGACAAATTTACCCTGGTTCTTTTGTAAATATTAAATTTTGAGAAAATATTGGTTTTACATCAGACAATTTTAAATTATTTTTTGGCAGGGATTGATGATTAGATGAAGACAAACAAATTGAAATCTATAGCTTTGATAGTTCCAAAAAATTTGAAGTTTTAAATGACGCTGGTATTTTCTTAAAAGATAATGGCGAATGAGAAAACAAAAAAATTAGACTTACTATAAAAATAGATTATATGGAATCAAAAACATTAAATCCTATTTACAATATTACAAAAAATATGGACTTGTTTAGAGCTGAAAATTATATGGACACTTTAGATATTAATACATTTAATTTTGAATTAACAGAGAAAAGTTTGATGGGATTTAAAACAATCAGATGTTTAAAAGATGATTTTGAAATTTTCAAAGAAAAAAATATGGAAAAAACAACAAATAATAATGGAGAAGAAGTTTGATATTTTAAAAATCATGCAGATTATTTCAATGTTCCAGTTAATACTTATCCAAATATGGAATATAATAAATTAAGTTTTATTTTTGAATGCAATAATGGAACATACACATTAATTCCTGATAAAGTGCCTTACTTTTACGATGGAAATTATTTAACATATAATATAACAAAAATAAAATAGCATAAAGCTATTCCTCATTTTTAAGAGTATCTTATTGATATTCTTTTTTTATTTCAATTCTTCAAATAAATTTGCGCATTTATTTAAACCATAAAAACGCATTGCATCTAAGCCATCTTCTAAGTTTAAGTTCAATAGGTAATCAGAAATGTTTTTATTCATATCAACATTTAAATTTAATTTTGCTAATGCTTTACATAAAAAAGCTTGATCTTTTTCATCTAATAATTTTTCTTTAGTTTTGCCTTTAATTTGATCAATATTTTGATACATATTTTCAATTGAATCAAAAGTCTGAATTAATTTAATTGCACCTTTGTGTCCAATGCCATTAATTCCTCTTAAATTGTCAGAAGAATCTCCTGCTAAACCTTTATAATCAGCAACTTGACTTGGTTTAATACCGTGAATAGTTTCAAAATTGTTAATGTCTTCTAATTCATAAGAAACTTTGAAATTTTCTTTAACTACTTTAATTACACTAGTATTAAGATTAACTAATTGTAAAAGGTCTTTGTCTTTAGAAAAAATTAAATTATTGCAATCTTGATTTTGAGCTAAAGTTGCAATTAAATCATCAGCTTCATCACCAACTTGTTCAAATCATTTGATATTTAATTTTGTCAAAATGTCTTTAATTAAACTAAATTGTTCAAAAATTATTTCTGGTGCTTGAGTTCTACCTGCTTTATAATCTTTGAATATTTTATGTCTTTTAGTTTCTCCATGAGCATCAAAAGCAATAAATAAGTATTTAGGTCTAAAGTATGCTATTAGTTTACTTAAAGTCATAAGAAAAACATGAACACCATTAGTTGTTAAACCTTGTGGTGAACGCATAATATTTTCTAATCTATCCGGATTATATGAAGCATAGAAAGAAGAAAATAATAAATAATTCCCATCTATTAATAAAAAGTTATCTTTATTCATTATTGAATTCCTTTTCAACTAATTAAATTATGTTTGTTACGACTGAAACTAATTGTAGCTTCAATTATTTGTCCTTTTTTAAGAGGAAGTGAACTTGCCAATACTTTGTTGTTAACAAAGAAAGTAATTTTAGCTGTACTATCTCTTAATTCTAAAACACTAAATTCTTTACCTCTTGGTCTTCTTAAATCAGCTACTTCAGCAGCAACAGAAACAGGAATTGAAGAGGTTATTGTATTTAATTTATTTTTGTATTTTGCTTCATAAATTGAAGTACTAAAAGCATTGTATAATTCACCTAAAAGAGCTATTTCATAGTCCATTTCGTAATTTATGTCACGTTCATTTTCTTGATCAAAAACTATATCATAGTAACCCATTTTTTTGATATCTTTTAATGCTTCTTTATAAGTTGAATGTGAAGATAAAACTTTAAAAATGTCTTTAATTTGATTATCACAAGATAATATATATTTCATGTGTCCAAAGTCTCTAAAAGCATTGGCTTTAATTAATATTTCCATAGCAGCATCTTTAATACCTGCAAAACGAAGTCTAAGCATTGTATCAATTAAATTCTTTTGAAATGGTCCATTTTCATTTAAATCAGCCACAATTTTATTAACACCTTCAGCTCCAAAACCTTTAATCATTTCAAAAGGTAAATAAATGCCATCGTTTGTTACTATACATTTATTACTTGAATGCAAAATATTTGGCGAATAAACTGGAAAATTAAGTTTCTTAATTTCAGCTACATATTTATTAATTGTAGCTTGTGCTCCAGCTGCATTTGTAATTAAAGAGCTATAAAATAAGTTAGCATAACGAGCTTTATAGTATGACATTTTCATAGTTAAATATGCATAACTAACAGCATGAGATTTATTAAAACCGTATAGTCCAAATTTTTCAATATTGTTATAAATAGCATTAATAATTTTTTCGTTTATGCCTTTTTTAATGGCATTTTCAAAAAATATGCTTCTATATTTTTTGATATCAACTTCACTTTTTTTGCTAATTGCTTTACGCAATAAATCAGCTTGAGCAAAAGTCATTCCGCCAATACTTTGAGCTATTTGCATAATTTGTTCTTGATAAACTATTATGCCATATGTGTTGGCAACAACTTTGTCATAATCAGGATGTATTTTTTTAATTAATTTTTGATTTTTCTTATTAGTTGCATAGTCATTGATAAAGTCCATAGGTCCAGGTCTAAATAATGAAATAATAGCATATAAATCATCAAAAGAATTCAAGACAACTTTTTGAATTGTCTTCTTCATGCCCGGAGATTCTAATTGAAATATTCCTTCAGTAAAACCTAAGTTGAGCATTGTTAAAGTTTTAGGATCATTTAAAATTTGAGGATCGCTTTTTACTAAATAATCAAATTTTTGGTTTTCTTCTAAACTTTTTTCAATTAGTTGAATTTCAGTTAAAGTTCTTAAACCTAGCAAGTCAATTTTTAAAAGTCCATAACTCTCTATGTAATTCATTGGTACTTGTACTTGTTGGTAATTGTCTAAATTGCTTAAACAAACAGGGACATAATTAAATAATTTGTCCTTAGCAATAATAAAACCTGCTGCATGGAAACTTTGTTGACGAGGTAAGCCTTCGATTTTAAGTGCATGTTTATATAACTCAGGATAATTATCCATTTCAATTTTAAAGTCAATATCATTTTTATAAGCATCAGTTAAGTTATCTGATTTAATAGTTTTACTGATGCGGTTGATGTCAATTAAGCTAATTCCTATAACTCTGCCCATATCTCTAATTGCCATTTTAGCACCAATAGTTTGAAAAGTTGAAATAAGTCCAGTTTTATCTACTCCATATTTTTTTTGTAAATATTCAAAAACTTCAAAACGACGATCATCTTGAATATCTATATCAATGTCAGGCCATGAAACTCTATCAGGGTTTAAAAATCTTTCAAAAAGTAAGTTATATCTTAATGGATTAATTTCAGTTATATTTAAAAGATAAGAAACAAGTGAACCTGCTGCACTACCACGACCAGGTCCTATTGCTATTTTTTGACTTTTAGCTCAGTTAACTAAATCTTGAATAATTAAAAAGTAGTTAATATAGCCAAGTTTTTTAATGACATTAAATTCATATTCAAGTCTTTTGTTTCACTCTGGATAATTTTTTAATTCACTTGTTAGTCTTTTAGCGCCTGCTTTTAAAAGCGAAACAAAAAGTTTTTCGTTGGTAGCTTCATCATTATTTTGAAAAGGAGCTAAATGCAAGGCTTTTTTAGGAAAAATAACATTACATTCATTGATTATTGAATAAATACGGTCAATTATTTTGCTATCTACATTAACTTCATCAAAATAGTTGGCATAGTGTTTAGTTTCTTCTAAATTGCCTAATTTTTGTAAGTAATTTAGTGTTTCATTATCATTAGCTTCAAAAAGTTTATTTTCTTTTACTAATATAGAGTTAGGCAATGAAGCATCTGAAGAATTAATATAGTAGTTTTTACTTGCACTATATTTATTATCTTTAGTTTGAGCATAATAACCATACTTTGGATGATCTAAAACTATTAAATTAGGATTTCACAATTCGCTAATTTCAATATCTTTATTGATACTTTTTTGATAAATTAGTTGATTAATTTTTTGGTAACCTTGGTAGTTTTTTGCTAAGACTATGAAGCGATAATTATCTACATCTAAGTCAACTCCTATAATGCCTTTGATATCATATTTTAAGCATGCTTCCATAAAAACACCTAAACCGAACATATTGTTTCGGTCACTTAAAACTACTGCAGGAAGATTGTGTTCTTTTGATAATCTAACTAAATCATCTATCCTAATTAAAGAATCTAAAAAAGTGTATTCACTTGTATTGTATAAATTGACATATTTTTTCATATTTTAATTATATTTATTTAATTAATAAAGCTAAGTAAAAGAATTATTTTTATATTTAGTTGTTAAAATAGTAAAATTATTATATACATTTCGGAGGTGTATTATGGCAAAAAAGATGAGCAAAGGTGCCATTATTGGTATATCAGTAGGAGCTACAATTGTAGTAGCTGGTGCAGTAACTGCTGCTCTTGTATGTACATTGCATGGAAAAACTGTTTATAAAATTGAATACTCTAAGTATAAAAGAGCTAGAGAAAAAGGCAATATTGGAGACAAAATAGCTTTAGCTACTTTAGAAGGACTTATTGTAACAGGTGAAACAACTTTGAAAGAAATAAATGGAACAAACCAACAATGATTGGATTTAGCTAAATTTGTAAAAGACACTAGAAAAGAAATAATAGGAAATAAATCAAAAGCTGATTTTCAAAAATTAATTGATGATTTTAAAAATTTTTTAAAAAGTTTAAAAGTAAAAAAATAATAGGCTGATAATGCCTATTTTTTGTTTGCTTTTAGACATAATTGTTAACTAATTTAGTTAAAACAAAACGAATGTCAACTTTGTTTGTATTTACCTTTGGAGAAAGATAATTTAAGAATACTTTGCTTGTTTCAAAATGAATTTTAGTTGGTTCAATTTTCTTTAAATATTTATTCATTTCTTTTGCGTCTTTAAATTTTAAAAACATTGCATGATTGTCAAATGATTTAAGTGATGTTGATGAAAAATACTCATTAAGTATTAGTTCACGACCTAATAATGTGTTTTTAATTGATTGTGATAAATCAACTATTTTTTTGTCGAAAACAACTTTTTTAATGACCTTTTCGTAAAATAGGTTAAGTGATTTTAAACCAAATTTTGCATTGTTTTCCAAAGCTGTAAAAATGTTTTTGAGTTTTGTTCCTTCATAAATATCAAGCATTCTAATTGAATAATTTAAGAATTCACTACGATCATAAAAAGCTGCAAACATATTTGAAATTAAAAACAACTCAAACACTTCTTTTAGTTTAACAATATTTCATTTCTTTCATTTTGACAAAATAAGTGCTAAGTCACTCAATATGAAATATAAAATTGAATCACTAAGTGTTTCTTCATTATTTACAAATTGTAATAATTTGAAAATGCTTGATGACATTAGTCAGTATCTATTAGGTATATTTACACCACGATATTTTTCAACTAAACTAGTTGGTATTAATTTTGCATATTCAATTTCAACTTGGTTATTGTTAATTTGCCCCACAAAAAGATTTTCTTTGTTTATTATTGCTTTATATTCTTTAATTTTGAAAACATGATTTCTAGTCTTTTTGTCTGAACTAATAAAGTGAAGTTTATTTAATTCACGATAAAAGTTTTGATCTAATCACATTAAAGCTGTTGAATCTAATAATGTAATCTTTTCTTTATTAGTAACTGATTGGATAGTTTCTTTCATATTTGTGTCTAAATCAAATATTAATCTTTTTAATTCGTTATATTCTTTTAATTTAAGATTAACTTTTAAATTATTAAGCATATATTTGTAACTCTTCAAATAGTTGTAATCAAATGTTATTAAAAAGTTATAAAGGAAAAATCTCTTCAATATCAAATAAATAGATATTCCAACTACAAAAATACAATACAATGAATACAAAACAACTGTTATTACAAAACTATTACTTTTAATGAAAGGAAGTTTTCAATTATTTAAATAATGAAGTAAAACTCCCATAATAACAGATGATAAAACTAAAAGAAGAGCTATCAACCAATCTAATAACATCAAATTATGAATTTTGTCTTGTGATCTTTTGTATTTTTCAAAATATGGTTTAAGTTTCTTAATAAAAAGCATATATCCTCGTTTTCTTAATGAAATTATATCAATTTATTATTTAGATAATAAATTCTGCAAAATTTTTCTTAAAAATGAAAAAAGCCCTATAAAACAGGCTTATATGCTCATTAATTCTTTTTCTTTGTTGATTGTCATTTTGTTAATGTGTTCGATTTTTTTATCAACTTCTTTTTGAATTTCATCTAAGTATTTCTTTTCTTGATCTTCTGATAATTCTTCATCAGCCTTGATAGATTTGTTAACATTTTGTCTTGCATTTCTAATTGCAACTTTAGCATTTTCTGTAAATTTTGCAAGACTCTTAACCATATCTTTACGTCTTTCAGTTGTAAGAGGAGGAAAAGTTATTCTAACTTGATTTCCTTCATCAACAGGATTTAAACCTAAGTTAGCATTTAAAATTGTTTTTGCAATTTCATGGTTGCATGAAACGTCAAAAGGTTTAATTAATAATTGTTGTGGTTCTGGTACTGTAATTGAAGCAAGTTCTTCAAGAGGTGTCAAAGTATCATAATAATTAATTCTAACTCCCTTAATTAATTGAGGGTTAGCACGTCCAGTTGAAATTTTTGATAATTCAAAAGCATAATGATTAATTGCTTTTTCAGCCTCATCTAAAAAATCTAATAAATATAAATCTAATTCCATTATTTAATTACCTCTGTGTGTTCAATTTTACCTTCAAGAGCTTTGACAATAGCATTCTTTTCTTTAATGTTGAAAACAATTAAATTAATATTGTTGTCTCTAGCCATACTTGTGGCCGTTAAGTCCATGACTTGTAATTTTTTGTCTAAAATTTCATCATAAGTTACAACATCATATTTCTTGGCATTTGGATTTTTCTTAGGGTCTGAATCATAGATTCCACTAACACCATTTTTACCCATTAAGATAACTTCAGCTTTGATTTCTGAAGCAAATAATGTTGCTGCTGTATCTGTTGTAAAGTAAGGACGACCTGTACCGCCAACAAAAATAACTACTTCACCTTCTTCAAGGTATTTTAAAGTCTTTTCATTAATATAATTTTCTGCAACTTTTTGATCAATTGCAATTGATGATTGGATTCTAGTTTTTAATCCAACTTTTTCGAATCCACTTTTAAGAGCTAGACCATTCATGATAGTTGCCAACATACCAATGTAGTCTGCTCTATTTCTAGGTATTCCATTCTTTTCAGCAGAAGCACCTCTTCAAAAGTTACCTCCACCAATAACAACACTAACTTGAACACCTTTGTCAATAATTTTTTTAAGCTGTTTAGCAATGTCTTCTACTAAATCATAGTCAATAGCTAAACTTTTTTGTTTATTTACTAAACCTTCACCTGAAAGTTTTACTAATATACGTTTGTATTTCACTTTTTTTCTCCTACAAATTTAACTTTAATTTTTTATATTATAAATTAATATCAAATAATTGTGAGTATTTTTCTTTACAAATATTTTGTAAAAAGCAAAAATACATAAAAAAATATAGAAATTAAAATCTATACTTCTTTTAATCATTTATTTTTTTATTTTATTTTTAAAAATTGACTAAACACCGGAAAATATTCATCATTGATTTTTGCCAATTCGACTGTTTTTCAGTTTAAATCAGAAAAAAATCTAAAATCGTCATGGCAAGAACTCAAGACAATTAATCTTAAAAATCTGTCTTCTTTAATTGTATTAATTAGCTCTATTAAACCTAATATTTTTGACTTACTTCCAAATATTTTTGTTGCTAAATTTATCTTATTTTTTCCTCAAAAAAGCAGTCTATTATGAGTTTTAAAACTATATAAATTTTTGCATTTTTCTTTACTTGTGCCAACAATTAAATCAATATAAATTCTTTTATTTTGGCATTTAAAAAATGGACTTAAATCTTTTTCATTTGTTTCATCATTTGTTAGAAATATTGAATTATTAAAATATTTGAGGTCAAAAAAATCTTTTCACCACAAAGAAATACGAAAGTTATCAGGTGTAACTTTTTCATTTTTAGAGATTACTCTTGCTGTCATTGGGCTTAAAGAATATGGAATTTTGTTAAGTTCTAATAGTTTTTTAAATTCATTTCAAGCAATCCAAAGCTCATCTTTTGTCATAAGAATAATTATAAATAAACTTTGAAAAAAGCATTGTTTTTTTTACATTTACTATATAATTAATTAGATTATAATTTTTATATCAATATTAATTTTAATATATATTTAAATATATGGAGGAAGCATGGCTAAAAGAAAAGAAACATTTTTCGAACGTTTGACAAGAAAAAATGCCGAACACGAATATAAAAATCAACAAGCAATGCCTAAAAAGAAAAAGCTAAAAGGTATAATCCTTGGAACACTTTTAACATCTGCAATTGCAACAGGTATCATTGTTCCAGTTGTAATTAATTCAACAAAAGTAACATATGTAGATGCACTCAAAGATGAAAGTGCTGTTTTAACATTTACATTGCCAGGATCAAAAGAAAAACATTCTATTACAATCAAAGATTTAAAACATTCAAATAAAACTGAAGATGCTAAAGATCCAAGTAAATTGTTACAACAAATTAAACGTCTTGCGATGTTTTATCTTTATAATAAAGAACAAAAAGCCTCAGCTGAATATCAAAGATTATGAAATATGTCACGTGAAGGCAATGAAGAAGAAAATACTTCATTCAAATTAAAATCAATTGAAGAACTTAAAACAAAAGAAAGAAACAAGATTCTAGATATTCAAGAAAACACCAAAAGAACAGCCGGTTATGATAAATGAGAAGAAAAATTCAACAAAATTTTAGTTTCAAATTACAATGGAGCTAAAAACATTGATGAAGCAGTTGACTATCAAGTTTTTAAAGCTATTGAAAAAGATGCTTTAAGACGTTTTGAACTTTCAACAGTTGATGTTAATAAAATAGTAAATCGTAAGGCTAACCAAAAGATCTATGAATTAGATAAAGACGGAAATCCAGATACAAGCAAAGTTTTATTTGACACTGGTCAATTAGTTTTTAACTACTACAAAGAAAATGAAAACTATTTCAAAATTAAAGATGATTACATGTCATTTACAACAAATAGTTATGTTAAAGCATATAAAGATGCATCTGCATTTATTGATGAATACTACAAAAATGATAATCCATATGCATTCACACAATTTACAATTCCAGGTGTTGCACCTACAAAAATAGCTAAAAAAGGTGAAAAAGAATTAGAATGAACAATTGACAGAACAGCTTTAAGAAAATTATTATATTTCTACCCAGCTGATGAAAAACATGGAAAAATTCAATCTTATGAAAAAATCTTTACAGACTTTAAGCCATATTCACATTACTCAAAGATTATTACAGATGCTAACGGTACAACTAATTTACCTCAAGAAATTGTTGACTATACAACATTTTTAAGTTGAATATCAGCAGACGCTGATGAATACAAAAATAATTTTGGAACTAAAGGAATTCTTTCTCTAACCAATTTATTAAAATCAAATAATGATGAAGTTATTAAAGGTTTAATAACAATGCCAGAATTAGTTTATGGATCAACCTCTGTTCCCGAAATTGATTTAAAAGCATTATTTGAAAGTATTCAATCTAAATTAGAAACACTATTCCCTGAATTAGTTCCATTAAAACCAAATTATGATGCTACAGCTTCATTAGAAGAAAAAACTAAAAAAGTTGGAGATTACAACAAAAAACTTCTTGACATCATGAGAGATTTTGATAACAACAAGAAGAGTGGTTTATATGATGACAAATTAAAAGAAGAAATCATACCATTAATTGCTAAAGCTTTTGAAGGTGCTGACAAAAAAATAAGAACATTTTGAAAAGTTAAAGGTTTAGATAATGCTTACCTTTATTTAAATAATAAAGGTGCTACAATAGTTTACTGTCAATCATTGACAGATGCTGATCAAGCTAAAAACAGAGCTAAAATGCTTGAAATCTTAAAAAATGATTTTATTTTAAGCAAAAAATACAAATCAATTAATGCAACAAAATACAATGCATTAGATGTAATTTCTAAAAACTTAAAATCAGATTATTATGTAAATAAAGTTTTATTAGAAGATGCCGATTTTGTTAAATATCTCAAAGAACAAGAAAATATTTATGCGCTTGATGCACAAGGCAAAGCAGTAAAAGGTGTTAAATATTCTGATGATGATATTGAAAAATTGAAAAAATTGAATGAAATTAAATATAGAATTAAAGCTGAACAAAACATTTTGGATGTTAATAAGAAATTAACAACTTGACTTAAAGAACGTGCTAAAAATGAAAAAGATAAAGACTTGTTATTTGAAGGCGGTTACAAAGTATACTTTACAAACAATAACAACAGCAAAAAAGATGAAGTGGCAAAAATTATTAGTGAAAAAATTAAACAAATTTTGAAAGTTTTTAGCTAGGAATGAGGTGCATAATGAAAAATAAAAAATTAATATTTTCTTTAGGAAGCATTAGCACTTTAACTGCCTTCCCTATAGCTGCTGTTAGCTGTGGCCGTGTTGTTGAAACTCCATCTAAAAAAATTCAAGATGATTTTTTAAAAGCTGGTGCCAGAAAAGAAGAAATCAAAAACATTTGAAGACAAATAACAATTGCTTCTTTATATAAGATCAACGCAAGTAATTCATATAACACTGATAACTCTGAATACATAAAAACATTTAATGATACTAATTCACAACTTTATAAGGATTCTTTAGAAATTTTTAAAGTTTATGCTGAAGATAAGGTTTTCAAAAATGGAACTGATGGTTATTACTTCTATGAAAATTTATTAAAATGAAAAGAAAAAAATTACTTTACTAAAGATCAAGAATTAAAATTATCAGCTTTAAAAGCTCGTGAAATTCCTAATGAAGAAATTTTTAGAATTTTTTGAAATGCTGAAAAAACAGGAATTAGAGCCGAAATTGAAAAAATACTTTTAGTCCAAAAGTACTTTGAAATAAGCAAAAAAGAACAATTAGCTAAACTTGATGACAAATTTAAATACAATTCATCATTCTTAAAATATGAATTGGAAAATTATAATTTAACTAAATATGCAGTTCAAAAGAAACCTGTTCAACTTTGAAAGAAAACTGCAAAAACTAAAATTACATCTGAAGAAGATTTCTTTGTTGCTCAATTAAAAACAATTAAAAGTATTGACACATTTAATGCCTTCATGAAAGATTCAGAAGATGAAGACAAAAAAATAAGTGACATTGAAAAAATTACTGATAATGCATACGAAAATCAATTATTAGGTTATCAAGGCATTAACTTAAACTTTAGCGATTACAACTTGAAATGAGATGAAAAGAATTTACAAAATGCTAAAGCTAACAAACTTTATGGTTTCTATACACCTATTGAAGATAGATTAATTTTACAAGATGATGAATTATTTAAAAAAGGTTATAGTCCATATAAAGGCTCAAATTCAAACAAAGAAATCGTTGCTGCTTACATAAACCAAATTGCTCCTATTGGGCAAGCTGAAGTTGAATTGCCAAAAGAATCAAATGAAAAAGAAACTGAAAAAGTTAAACTTCTTTCATTCAAAGGCACAATCTTCCAAAATCATCTTAAAAAATTAGCAACCTTATTTGCTTTAAAAGATTCTTCATTAATTGATACAGCAGAAACTGCATTCACTAAATTAGGTTACAAACTTAAAGTAGAAAATGATGCATTAAAAGAACTACTTAAAGGCGAAAAATTTGTTGAAGGTGTAAAAGACAAAGACTCTGACAAAAAATAAGGAGTTAATTATGCAAAACATATTTCAATTAATAATTAACAAAAAAGCAGAAGCAAATATTATCTTTGAAGATGATAAATGTATAGCTTTTTATGACAAATTTCCAATTCAACCTGGACATTTCTTAGTGGTTCCAAAAAAACACAGTAAAAACATTACTGAAGCAGATGATGAAACAGCTGCGCACTTAATAAATGTTGCTCGGAGACTTGGAAAAAAACATGTATTAGATAAGAAAATTGCAGGCTTCAAAATAATAATTAATACAGGTGAAGCTGCTGATCAAACAATTTTCCATACACATGTACACGTAATTCCATATCGTGAAAAAGTACAAGATAACTAAAAAGATGGCTTAATAAAAAAGTCATTTTTTTAGTTTTTAGTTTTTAACTAAACAAAATGTGCCAAATTATTTATAATTTTATAAAAGTTATATGCAAAAGTACGTTTTTGACTTTTTGTACTATAAATAAGCAAAAAAATATAAAATAATAAAAAATATAGTATGATTATTATGCTATAAATCAAGAAAGGAAGATTATGACCAAAAAAGAATTTGTACAAAAAATTGCTGAAATGTTAGAAGTTCCAGTTAGAGTAGCAGATCAATACTTTGATGCATTTCTATTTGTTTTGAAAGAACAACTTATAGCTGAAGACAAAGTTCAACTTTCAGATTTAGGAACCTTCGAAACAAAAATTAGAAGAGGGCGTGAAACAATTAATCCATTTACAAATGAACCAATTCAAGTACCTGAAAAACGTGTTGTAAAATTCACACCTTCAAAATACTTAAGAGATATTGTAAACTTCTAATATCAACAATCACTAATAAAAATTTTAAACATAGGGCAAAAAGTCGATTATACCTGGAAAGGTTAATTCGATTCTAAAACCTATGTTTTTTATTTCTTCATAAGAAATAGATTTATTCATATGCTGAATTAACTCACTTGCTTTAACAAGATAAAACTCATCACAAAAACTAAAAAATATAATAAAGAAAGCTAATCCACTATTACTTTCAATATCCAATAAATACTTAATTTGGTGCCGGGCAATATTATTTGCTGGCAATCTTTTTTCATTTGTGCTTTTTGCTTCAAAAGCAACAAAAAAACCTTTATAACAACCAATGTAATCAACTGTACTCTTCTTTAATATATAAGCCTCATCCAATTTCAAATTATCATTTTCAACCTTCTTAAACTTAACAGGTAAGGTCTTCTTTTCAATATAAGCTAATTTATTTTTGGCATAGTAATCAATTGTTTTATTAATTATTTTTTCTAATAACATTCCTCTATTTTTTTGCATGAAACACCTCAAATTTGAATTAACCTTTTTTATGAAAAACATATCAAAAATGGAAAAATTTAGGAAAAATAGACTTAAAATACCAAATTTTTCCTAGAATCAACCAAAAATTAAAAAAAGCTAAAGTATAAAATGGAATGAAATATAGAAAGATAATATTAGGAACTTTGTTAGCAGGCACAATTGTAATTTCGACAAGTACAATTGCTTTTAATATTCAAAGAACCAAGGTTGCAAAGGTTAAAAGCAATGATCCTGCATCAATAGGATCAAAATTTTGTATCAAAGTATCCGGTGCAGTTGCCTTTGCTAAAAATTATTATTTCGAAAAGCCTATTTATTTAAAAGAATTATTAGATATGGCCCAACCTTATGAAAATGCAGACTTAAGCAAAATTAACTTAAAAAAAGAATTAAAAGAAAATCTTAATATTTACATTCCATATAAAAAAACTGATAAACATTTTTTGTATTGAAATAACTTAAGTAAAAAACACTTTAAATTGTTAGTGGATTTGGGCATTAAAAAATCAATAGTTAACAAGATTTTGGCTTTAAGAGAAAAGAAAAATCAAATAACATGGAACGACTTAGAAAATATTTCGGGAGTTGGCAAGGTAACCTTAAAGAAATTACAAGAAATTTTAATTCTGTAAGTCTTTTTCTTTTTAGTTTACTCTCGCCCATTTTATTACACTTAGTAATAAATGCTCAAGAAACCAAAATATTTTGAATACTATTATTTTTAATTTATGTTACTTTCTTAGCTTTAATTAAGCCTAAATATTTAATTTTAGTAGCCGTGCTTTTGACTTTATATTTAAGTTATTTTTTATCTAAAACAAAAATTTATTATGAAAAAGGTATTTATAAAATTGAAGGATCGATAAGCGTCCTAAGCAAAAAATATTTAGTTATATCAAATGAAAATAATAATATTTTAATTTACACAAGTGATTTAAAAAATAGCACTCCTTTAAGTCTAGGGGACCAAGTAGAAATTCAAGGTGAATTAAATCCTATTGAAATAGACAATAGTAAAATAGCAACATTTTATTTGCAAAATAATATAAATTATTTGCTAAAAAAAACAGTCATTTTAACTGTTATTAAACCAGAATTTTCTGTTGAAGAAAAAATAATGAATTATTCAAAAAATCATGGTGAATTATTTGATTCATATTGAAGGTTGTTAGTTTTTGGATCCTATGATTCAAAAGAATTAGTATTGAATAAAGTTAATAGACTTAATATTGTTCATTTAATTGTTATTTCAGGTTTGCATTTTGACTTATTATTCTATTTAATTTTGTTGATACTAAAACCTTTGGATAAAATTAAAATCAAAAGTAAATATATGGCTTTTGCCCTCATATTCTATTATTTAACATTACTTGCTAATCCTGTTTCTGCTTTAAGAGCTTATATTATGCAAGTTGTTAAAAATTCAAATAATCCTTTTAGTTATAACAACAAATTTAAAAAATATGATGGTCTAGTAATTTCGATTTTAGTTTTATTTTTACTTAAAAATAATTACTTGTTTTCTTTATCATTTATTCTTAGTTTTTCATCAACTTTTACAATTATGTTATTAGTGCCAATGGTCAAAAGTAAAAAATTAAACAACTTTTTAAAAACATTAATTTTGATGACTTTAATATATCTTTTTAATATTCCAATGCTTTTATATATTAATGAATATTGAAATTACTTTGGATTATTACTAGGAATAATTATGGCTCCATTGTTTCAATTTTTTCACTTGTGTTCTTTATTATTTTGATGAAGTCCAACAATGCTAAGTTGAATGTATTACTTGTTAGATAATTTATTATATTACTTAGTTGAATACACTATTGCTTTAAAAATAAATATAAGCGTCAATTGATGAATATTAATCTTTTGAAGTATCTTTTGATATTTAACAATCTTTTTTATGAAGCTAAAATCTAATATAAAAATTTGAAAAATAAAAACAGCCTAAGCTGTTAAGGGTCATCACTGATGAAATGGCGTACCCAGCAGGAGTCGAACCCGCAACCTTCAGGGCCGTAACCTGACACTCTGTCCAGTTGAGCTATGGGTACATAATGGAGACACCAACGGGATTCGAACCCGTAATCAGAGTTTTGCAGACTCATGCCTTGGCCATTTGGCTATGGTGTCATCTGCAAATAATTATATAATATACGACTAAAACTAATTATACAATTATATTTTTTTATTTAAAGAAAAAAAATAATTACTATTTTTGTCAATTAAAACTGCAAATTCTCCATTAATAAATTTAATATTTTTTGCTTTTTAATAATACCACAAAAAATATTTATTTTTTATTTTATTTATAGCCTTGGTATTTTTAGCCTTAAAATTGTTTTTCAATTTTGATAAAACAACTATAAAACAAACTTTTATTTATATTTTTTAACAAAATCTAAAAGTAATTCAGGTTTCTTGTTTTTATATAAAATATCAATTATGCTTTTTAAAAATACAATTTTAAGATCTTTATTTTTCTTTAGAATATTAGCTAATATTTTAGCAGCATGATATCCTTCAATAGTTTTCTTATTATTTTTTAAAGTTGCTTCCAAGCCAAATTCTGCAACTTGTCTACCAAAAGAAAAGTTACGGCTTTTTGTACTTGAACAAGTTAAAAAGATATCGCCAATTCCCGCTAATTCAAATCCCATATTGTCATTTGATTCAGGAAATATTCTCTTGTAAACCATGTGAATTTCTTTAACACCAATTGAAAGCAAGGCCGCCATTGTATTTCGATATGGTCTTAAATAATCAATCATTCCAATTCCAATAGCTAGAACATTTTTTAGAGCAGCAAACAATTCTGAACCGTGTTCATTTTCATTAACAACAAGTCTAAAAGTGTCATTATTAAATAATTCAGAAACTTTTTTTAAAAATCTTGTTTTTGGACCAACAACATTAATCATTGTTAAAACATTTTCAAAAACTTCAACAGCAAAAGAAGGACCAATTAAAGTACAATAATTTTTTAAATTATTCGCAAATTGTTTGTTTAAAACTTCTGAAAAAAATTCATTTGTTTTTTCATCAATACCTTTTGCAACATTAATAACATTTATTTTTTTATTTTTGATTATTTCCTTTATTTGTTCTAACACAGAAGTAATTGCACTTGAAGGTACAGCTAAAATTAAATAATCAAATTCTTTTAAAGCTTCATTTAAGTCCAAAGTAGCTTTTACATTAAAAGGATTATTAAATAAAGTATCTTTAAAATATTTACTATTATTTCCTTTGTTAATTGAATCTACTTCACTTGAATCAATTCCTCACATTAAAACCTTGTTATTATTTTTACTTAAAACAGTTGCAAGTGCTGAAGCTCAGGCACCTGTTCCGATAATTGTTACTTTTTTCATAATGCTCCTAAATTATTTAACTATTATTGTGTCATCATCATTTTTGTTATTAGAATTTTTATCACTGTAATCTTTTTTGCGTTTAAAAGCAATTGTACAATATGCTTTATAGTATTTAATAGCTTTTTCTTTTTGATCACTTTCATAATACTTGATGGCTTTCATTATTAAAATTCAACCAGATAATTGCAAAGTTCCAAATAATGTGAACACTGTGAAACATAAGAAAATTGCAACTTGTGCGCCAACATTAAAATAACCAAAGCCAAAAACCATTGAAAGTGTAAATCAAACCGCAAAAAGTCCAAAAGCAATAAGATAGCAAAGTGTTACTAAAATAATTATGAACTTTTTGGCATTTTTATAAGCTTGTGCTTCTTCATGATAGTTTTTACAAACAACAATTAATTCTTTTTCATATTCTAAATCTAAGTCCATAAATCTCCTAATTTTCTAAGCAATCGTATAAAGCTAATATAGTTTTTAAATCTAATTCTTGAATTCTAGTTAATTCTTTTAAATTTAATTTATTATATGCAATTTTAATTTTATCATTACTATATTTTGTAGCTAAAGCAAATGATAATTTTTTTCTTCTAGCTAAAAAACATAATTTGAAAAAGTCTTTTAAATTTTCATAATTATCATTTTCTTCATTTTTAAAAGTAATAGAAACAATAGCAGAATCAACTTTAGGAACTGGATCAAAATAAGTCTTCTTAACAAATAATTCTTTTTTAACATCAGCACAATATTGAACTGTTATTGAAAGTTTGCTGTAATCATTTTGATTAGGTTTAGCCACTAATCTTTGAGCCACTTCATTTTGAACCATAAGAATAGCTTTTTTAAAATTATGTCGATAATCTAATAGTTTAAAAATAATATCACTAGTTATGTAATAAGGAATATTTCCAACAACAACATAGTCTTTATATTTTTCCAAATCAGCTATCAAAAAGTCTCCTTGGACAATTTGGTTTTGATTTAAGATATTATTTTTTTGTAAATAATCAACCATATCTCGATCTATTTCAAAAGCAGTAAATTCTTTTGCTTGTTGATTTAAAATTTTAGTTAAAGCACCTCTTCCAGGACCTATTTCTAAAATCTTTTCACCTTGTGGTTTAATCAATTCAATTATTTTGCTAATAACATTTTGATCTTTTAAAAAATTTTGACCATATCTTTTTTTGGCTTTTAATTTATTTTCTTGAAACATTTATCTTAAATAAATCCTTTGCATTTTTTAAAATCTGGTCAGTAAATTTTTCAATTGGTATTTTTTTAATACCTGCAATAAAGTGAGTTGTTTGAATTACATAATTTGGATAATTTAATTCACCTCTTTTGTGAGCTGGAGAAAGAAAAGGAGCATCAGTTTCAGTTAATATTCTATCAATTGGCAATCAATCTAAAACTTCAATAGTTTCAGTTCCATTTTTATAAGTTGCCACTCCGCTAAAAGAAAAGTAACAACCTAATTCGTAAAACTTTTTGGCTCAATATAAGTTACCACTATATGTATGAATCATAAAAACAACATCTTTATAATATTGTTTAATAATTTCGTACAAATCTTCATATGCATCACGCATGTGAATTACTACTGGTAAATTGTGTTTTTTAGCTACTTGAATTTGAGCATGCAATGATTCAATTTGAATATCTTTCTTTTCTGGTGTATAAAAGTAATCTAAACCAATTTCGCCAATTGCAACAACAGAATCATCTAATTGCTTTTCAATAATTTCGCCGTCAACCTTTCCATGACATTCATTTGGATGAATTCCAATAACAGGAAAAGTATATGAAAAGTTTTTGGCAATTTTAATAACTTCTTCATTTTCTTGTTCATTACATCCGGTAATTAATAAAGCTACTAAACCTTTAGCATGAGCTCTTTCAATTACATGATAATTGTCATCATAGTATTCTTTTAATGGATGAGTATGAGCATCAATATATTTTATTGACATAGTTGTTCCTTTCTTATTTCTATTTTCCTAAACAAAAGTTTTTAAACATTTCGTCAAGCAATAATTCATTGTCAGCTCTACCTGAAATATCAACTAAATCAGCTCAAGCTTGTCTTACATCAATAATTACTACATCAACATCAATGCCATTATTTATTGATTCAATTGCATCAGAAATACTTGAATGAGCCTTTTTAACCAATGCTAGTTGTCTTGCATTAACAACGTATTCTTCATTGTTAATTTCATTCTTTGAAAAAATATTAACTAATGCTTCCTTTAAATCATCAATGTTTTTATTTTTAGCACTGACATAAATAACATTCTTTTTCTTTTTATTATTTTTTAATAAATCAACTTTATTAATTACTTTAATTAATGGTTTGTTAAATTGTTTAGCTAATTTTTCAACTTTTTTATCGTAATCATTTTCTAAAGTATTAGCTTCATTAACATGAATTACAACATCACATTTTTCAATTTGTTCAAAAGATTTTTCAATACCTATTTTTTCAATTTTTCGATTGGTTTTTCTAATTCCGGCGGTATCAACTAATTTGAATAATAAACCATCAATTTGATATGAAGCTTCAACAAGATCACGAGTTGTTCCAGCCTGATCTGTAACTATTGCTTTATCTTCATTTATTAAGCAATTTAATATTGAACTTTTTCCAACATTTGGCTTACCAAGAATAGCAACTTTAATGCCATCGAAAATTAATCTTGAGTTTTCACTTTCTTTGATAATTAAGCTCAATTTGTCTTGCAATTTTTGCAATCTAGAAATTAAATCTTTTGTCAAAACATTTTCAAAATCTTCATACTCTGGATAATCTATATTTATTTCCATTTGACCTATTAAATAGGCTAATTCGTCAATAAATGATTTAATCATGTTTGAAGTTTTGCCATCAAATTTCTTAATGGCTAATTCACTTTGTTTTAATGTTTTGGCATGTATTAAATCATTAATTGCTTCAGCTTTAATTAAGTCCATTTTACCATTCAAAAATGAACGTCGACTAAATTCACCTGGCAGTGCCATTCGAGCACCATTAGCTAATAATAACTCAAGAATTCTATTGGTTACTAATACACCACCATGGCAATTAATTTCAACAGTATTTTCGCCTGTAAAATTTTTATTTCCTATAAATCAAGCAATTAAAACTTCATCAATAATTTCTTTTGTTTTATTATCAAAAATATGGCCATAAGTTATTGTTTGATTTGTTCCTATTTTTCCTTTAAAAATTTTAGAAACTATTTCAATACTATCTTGACCACTAACTCTGATTATTGATATGGCTTGATTAATCTTACCACCTGAAGATATTGCTGCAATTGTGTCATTTATCATAATGTAAATATTTTACATTAAATTTAGATTTTTGTTTGTATCAATAAATTGACAAATAAAGGAGAAAAAAATGAATAATCAAAATTTTAAGAATTTATTAACTAAATATATTGAATTCTTTTATAGGTATAAAAGCATTTCATTATTAAAAAAGGCAAATAATGAATCACAATTTGATGTTAAATTAAGATTAGTTTTAACACAAAAATTTTTACTGCATTCACTAGGTTTGCAATACAACAAAAAGTTATTTAGAAAATATAAGAACAATAATTTTGAACTTTATAGAGATATAAAAAATGATCAACTAAATTATGATGAGTTAATTAAAGACATAAGCAAAAACAAAAAAGATTTAATTGACAAAAAATTAAACGCTCTTGAGATTTTTGTCAATATTATAAAAAATATTAATCAAGAAAAACTATTTACTTTTGCATTTGAATTTTTTAAATTAAAACCAAAATTTGATATTGATGGAGCTTATAACCATTCACAATTGATTTTAGTTACAAAAAGCAATAAAGAAAATCAAAGATGTATTATTTATGGAATTTTGGGAGATTTATATACCACCAATTCAAAAGATAATAATCATTTTTTAAACGAAAATAAAAATCTTATGAACACAGTTTTTATACCTATATCAATAAAAATCAAAAACAATGAAAATCTTTTAAATTCTCAAAATATACAAAAAATTGATATTGCATTTTTAAACAAAAGAAGAATACAAGAAAAAGAAATAAGAGTATAAGAAAACTTGCTAATAAGCAAGTAATCTTTTATTTATATTATTTATTTGATTCAATTTCATTGTTAATGTTTTCAGCTTCAACACCTACAATAATTTGAATTGAATTCTTTCCTAATCTAATCAAACCACTGCATCCCGCAAGTTTTAATTTTGCTTCATCAACAATATCTGAGTCTTTAACTTCATAACGAAGTCTTGTTGCACAGTTTGCAAAAGTTGTAATGTTTTCGTAACCACCAACAGCTTCAACAATAGCTTTTGCTTTTAGACTTAGGTTATTTAATTTCGAATCTGTTGGAGTTTCTTCACCAGAAGCACTTGAAGTTGATTTAACTTCTGTTAAAGCATTTGGATCAGGCAATTGGTTAGCACCACGACCTGGAGTTTCAAGTTTAAGACCTTTAATTGTATAGTAAGCAACAAAGTAATATGTAGCTGCACATACAACACCAATTACTAAGATTCAACCAGGGTTAGCTAATACAGCATCAATACCTGTTTTGTTAGCTTTAATAATTGCTAATGATTTAGGAATTGATAATAAGTAATCCATTAAACCAGCTGAGAACCCAAATCCAATTTGAATTCCAAATGCACCAGTTATAAATGCAAATATACCAGTTAGAATGCAGTGGAAACCATATAATAATGGACTAACAAACATAAATGCAAATTCGATAGGTTCAGTAATACCTGAAAGAATTGATACTACTGCGGCTGCTCCAAAGATAGCCATTACTCTTCTCTTTTGCACTTTATCATCTGCAGTCATGTAGAAAGCAAATGCTAGAGCAGGAAGACCAAACATCATAACTGGAAAGAATCCTGATTGGAAGATACCAGCTGTGTTTCCTTTTGCTTTACCACTTAAGAAAATAAAGATATCACCAAATACAGGTGTACCATCAGGAGTTACATGTTCTCCTAATTGGAATCAGAATAAGTTGTTTGGAATGTGGTGTAATCCAAATGGAATTAATAGACGATTAATAACGCCATATACACCCATAATAGCAGCTGAAGCTCAACGACCTTTAGCTGCACCTGATAACACTTTTGAAAATGCATAAATACCATATCCAATTCATGGAAAAATAGCAGCATATGCAATGCCTCAAATAAGAGTTGAAAGAATTGCAAGAGCTGGAACTAATCTTCTACCCGAAAAGAATCCAAGAACTTTAGGTAATTGAACATCATTTGTATGGTTATAAATAGCTGCAACAATAAATCCAACAATAATACCATTAAGTACATTGCCAGACATTATTGCATTGTATTTGCCACCAAAGACACCTGCAAAACCATTAGCTATAACAAATTCTGAAGCAGGTTTACCTTCAGCTATAAAAGCTTCAAGTTTTTTACCATCTTCATTAAATTCGGCTCCAGGGAGTTTAAATGTGCTGTAGATTTTATCTACATATCCACCACTTTTTAATAAAAGTGAAAGTAATAGAATACTAATTAAAGCAGCAAAAGCAGCTTCACCACGGTTATCTTTAGTAAAACCAAATCCTATTCCGACGGCAAATAAAATGTACAAGTTGTCAAATACAACTGATCCGCCTGCTGTTATCATTTGTTGAACAAAGTTAACAAAGTTAGCTTCGACTTGTGAAATATCAGGTCCAGCTTTAACTGTTGGTAAATCAGCTCCAACTCTAAGAAGAATAGCAGCAATAGGAAGAACTGCAATAGGGAACATCAATACCCGACCAATGCGCCCAAGTTTTTCTAATAGTCTTTTTCCAAATGAAGGTCTTGATGATTGTGTTTTTGTACCAGTCATTATTTGCCTCCTTGTTTTTGTTGTGCTTTTTTTGTAACAGCTTTTTTATCTAAGATTGATAAAATAACTGTTCCAAAAATTGCAGCACATAAAGATATAAAGAAAAATAAGGCAACAATGGCTACGTATTTAGCATTTGTTGTTTTTAAAGATTCTGCAGCATCGAAGTTCTTAATAGAAGCTAAGATAATAATTAAAACCATAAAAACAAGAAACACCATTCAAAGTGAAAAAATAATCCACTTGTTTCGAGTCATGTTTTTGAACATAATTTTTCCTTTCATGTATATACGTTAGTGGAACCTTAATGCATAGTTCCATATATATTTTAATATTTATATTAATTAGAGCAATTTAGCGACACACAAATTTGATTAAAAAGCCTTAAAAATAGGCGTTTTTTATTATTTTGACTCAAAATTTGTGAAATTTTTCACATCATTAAGGGCTGAAAAAACCAAAAAAAATGCGAATTTTGGACTACTTTCCAAGATTCGCAAAATATTATTTTTGATTATTTTTCAACTGCAAATAATAAGTTATTTACTAAAACAACTGAACCAGTTTGGTCGCCTTTTAGCAAATCACAAATCACAAATTTAATATTTAACTTGCCTTTAACAAATTCTAAGTTTACTCCGTTTGCATGAGGTTGAGTTTTACCACCATATTTATTTTCATAAAATGTTGAAATTCTAGCAACTTCGAATACTTTTGAAGTTAAATAAAATGCCTTAAATTTTTCAGTAAATTCAATAATTGTATAAGCAGATCTTCCGATTTTATGTTGTGCAGGATAAACTTGAGCAAATATATCACCATTTTCTAGTTTTTGTTTAGCACTAGCAATCCCATCTTCTTTAATTATAAAAGCACTTCCGTTTTCAACCATTGCTTTAATTGTTCTAGCTAAAACTAATGGCGCTTTTTCTTTAATAGTTTTAATTGCATTATCTACATCAGAAGGATTACCTTTAGCAGTTAAATCTACTCCTTCTTTTTTAAATTCTTCTACTAATTCCTTGAATTGTTTTAATTTTTCCTTGTCAGTAATTAGTGCAGAATATTTAGAAGCATCTGCATCCTTTGTCAATTTTTCCTTTAAAAGATCAGCTAAAGTTGCTTCATCTTTACTACCACATGATGAAGCAACAAATGGTATAGCTGTAATAACTGGAGCAATAATTCCTGTTAACTTTAATAAGTTTTTCTTCATAAATTCTCCTTTAATTCTATATATCTATTATATCAAAATGCTAAAAAGTTAAAATGGCAAATAAAAAAGCACTCATATTGAGTGCCTTGAAAAAAATGATTATTTTAGTTTTGCAAAGTATGACAATGTTCTTACTAATTGTGAAACGTATGACATTTCATTATCGTATCATGCAAAGATTTTGTAAAGTCTTTGACCATTTCCTTCTTTAACTTTTGTTAAAGTTGAATCGAATAATGAACCATAGTGTGAGTTAATAACGTCTGAAGAAACGATAGGGTCAACTACATATTTCATTGTTTCGTTTTCTGCTTTTTTGAATGCAGCATTAATTTCTTCAACTGTAGGTTGTTTTTTCAATTGAACTGTTAAGTCAACGAATGAACCTGTAATTGTAGGAACACGCAATGCTGAACCATCTAATAATCCATCAGCTTCAGGAACAACTAAACCAATAGCTTTTGCAGCACCTGTTGTTGAAGGAACAATGTTTTGAGCAGCAGCACGAGCACGTCTTAAGTCACCTTTACGGTGTGGACCATCTTGTAACATTTGGTCACCTGTGTAAGCGTGAACTGTTGTCATGTAACCGTTTAATAAACCAAATTTGTCTACTAAAACTTTAACAACTGGTGCTAAACAGTTTGTTGTACATGAAGCACCCGAAATAATTTCATCTTGTTTTGTTAAGGTTTCGTGGTTTACGTTGTAAACGATTGTTTTAACATCTTTACCAGCTGGAGCTGAAATAACAACTTTCTTAGCACCTGCTTCGATGTGTTTGTGTGCTAATTCTTTTTTAACAAAGAAACCTGTACATTCTAAAACAATGTCAATTCCTAATTCACCTCATGGTAAGTTAGCTGGATCTTTTTCGCAGAATACTTTAATTTCTTTTCCATTAACTTTAATAGCATTTTCTTTAACTTCAACCTTAGCTTCCAATGGGCCAAAAGCTGTATCGTATTTAAGTAAATGTGCTAACATTTTTGGATCTGTTAAGTCGTTAACTGCAACAACTTGAACTTCTTTATTTTGGGTTTCAAGTAAGTGACGAAGTGTTAAACGTCCAATACGACCGAAACCGTTGATTGCAACTTTTTTCATCTTTCTCCTTAATTAAAAAAATGTAAATTTATAAAAAGCTTTTTATACGCAATAGCGTTGTATTCATATGAATAAATACATTTTAATTATATATTTTTTTAAATAATAAAATCATTTAAGTAAGCAAAAAAAATGAATCTTGTGCAAATGCTCTCACAAATAAAAAATAAAAGGCTTTGAGCCTTAAATTATTAACAAATAGTATTGCCTTCATAGGTCTTAATTTCTTCACTAAAATATTTAGCTAAAGATTTTCATTGTAAAATTACTTCTTCTTTTTTAGCAAACTTCTTATATTTTTCTAATGTACTTCATCTTTCCATAAAAATGAATGTATTATCATTTACTCAGTAGCCATCAAAAGATAAGTTGTATTCTTCTTTTTTAACTTCATCAATTCATAAACTAACAGTTTTTTCAAAAATCGATTTGCTTTCTAACTTGATTTTTACTTTTCTAAAAATTGCATAAATCATTCTTCCTCCTTTATTTTAATCTATTAAATAACAAATATTTCTCATCACAAATAACTTTATCAAATTTATTGAAATCATTAATTTTTTCAAAGTCAAATTTTTTAACTTTTAAAACACTTGCAACTTCTTTTATTTTTGTAGGCAATACTATTTGTAATGCACAACTAACAGCATAAATTCCATTTAATAATCTTACTAAAATTGGTTCCAATTCTTCTAATTTATCAGTTAATTTTCATGGTGTTGTTTCATCAATATATTTATTTAATTTAGCTGATAATTCAATTGCTTTTTTAAATCCTTTATCAATTTCAAAAGCGTCCATATGTTTTTGAAATTGAGCACTGAAATTCTTAATTTCTTTTTCAATGTCTTTATGTATTTTTTTACTATTAACACTATAAAACAGACCTTTTGGGAAAGAATTGTGAATCATTTTTAGTGTTCTTGAAACTAAATTACCATAATTATTTATTAAATCTGCATTAACAACTTCTTTTAATTTTTCTTCACTAAAATTACCATCTTCTCCAAATATTATTTGAGTTGCAAAATAGTATTTAATCATCTCATGATGATACTTGTCAAATAAATAATCTGGGGCTATAACATTATTTAAAGATTTGGACATTTTTAGTCCATCTTTATCTCTTAAAAGTCCATGACTAACAATATGATTTGGTAATTTAATACCTAAACAATTTAAAACAATTGGTCAGTAAATAAAGTGAAATCTTGCTATTTCTTTACCTAAGATATGCACAATTTCATCACCATTTTTTCAATATTTAACAAAGTCATCTTTTGGTTTATTGAAGTCGAAACCAAGTTTTGTTATGTAGTTGAATAAAGCATCAAATCAAACATAAATTGTATGTTTAGGATCATCAATTGTTGGAATGGCTCATTTAACATTTGTTCTTGTAACTGATAAATCTTCTAAGCCTTCTTTAACAAAATTGTTTGTCATTTCATTAACTACTTTTTTAGGCAATAAGAAGTCTGGATGTTTTTTGTTATATTCGCTTCATCATTTTTGAACTTCCTTCATTTTGAAGAAATAACTTTCTTCTTCCATATTGATTAATTCATGTTTTGATGAAGGATGATAATACTTGCCATCTTTATAAACCGCTTGATTTATTGTCAAAAATTCTTCATCTTGAACTGAATAAAGTCCTTGATATGAGCCTTTGTAAATTAAACCTTTTTGTAAAAAGTAAGAAAATATTTTTTGAACAGCTTGTTCATGAAATGATTCTGTTGTTCTACTAAAATAGTCATAATCGATTTCTCATTTAGCTCACATTTCTTTGTAGCTTTTAACTAAATCATCTACATATTGTTTTGGTTCTTTTTTAGCAACTAAGGCTTTTTCTTGGATTTTTTGACCGTGTTCATCACTTCCAGTTAAAAGCTTAGCATCATAACCTAAAGCTTTTTTATAGTTTCTAATAACTCAAGCCATAATTGTACAATAAAGATGTCCGATATGAAGCGGACCTGAAGCATAATAAATAGGAGTAGTTATATAAAAAGTCTTTTGTTTTTTCATGTTTATTTACCTTCTTTAACTTTAATTGGTTTGTACAATTTTTTAATATTTTCTAAATATTCATGTTGCTGTTTGTCATTAGGATCATGCAAGTACAAGTTAGGCAAGAAATGAACACCTCAACCAGCCTGATATCTGCCTTCAACTAAAACTAATTTAGGTTTATCTTGCATTCTTGGAATAATGAATTGAACTCTTTTAGGCTCAAAATTATATTTTCTCATTAAGCAAAAGCAATCAACTAAGCGTTCAACTGGAATCACTAAAGTTAAGTAGCCTTTTTGCTCAATTAATTTTGAAGAACCTAAAATAATTTGTTCTAAATTAATTTTATACTCATGTGTTGCAATTAGTAATTCTTCACTAATATTTTTGCTAACTTTATTAGTTTGCATTGTATAAAAAGGAGGATTGCAAACTATTGAGTCATATTTACTAGCTGCATTTTTAATTTTTTCTAAATAAAATTCATTGAAATCTTGATTAATTATTTTGATTTGATTTTGCTTGTTGTTAAGTTCAATATTGTCTTGAGCTAACTTACAAGCTTTAGTTTGGATTTCAACTGCATCTATTTTTAAATTTGGATTTCTTTCAGCTATAAAAATACTGAGAGCTCCATTGTTTGCTCCAATTTCTAATAGGTTTTTAATTTTATTATTTAAAAAAACAAAGTTGCCAAGCATAATAGTGTCAACTGAATAGTTGAACATATCTTTGTCTTGCACAACATATAAATTAGAATCAAAACCTAAAGAATTTTTAACTAATTTTTGTTTTTTATTCATAATTATTTACCTGATTTTTTGTGTTTGCGATTTGAGTTTGAATTAAGTTTTTTCTTGATTTCTTCTAAGTATGAACCATAGCATTTTGCAAGCACTGCATCAATTTTACCGTTAGTTTCATCAGCTCCAGCAATAACCACTTCTACTTGGTCTCCAAGTTTGAAAGTGTGTTGTTTACTTCTTAATTCAGTAAAGTTTTCATTAACTTCATAATCACCATCACATAATGTTGAAATATGAACTAAAGCCTCTATTTTGCAGTCAAATTGGACAAACATACCAAAGCGTAAAATACTAATAATTTGAGCTTTCATTGTTTGACCAATTTTGTTTTTGAAAAACTCAGCATATTTTAAATCATTTGTATCACGTTCAAGTTGTAATGCTTCTTGTTCACTATTTGAATTTAATTCTGCAACATTACTTAAAATGTTTTTGTAGTGAGTAATTTTACTTTTATCATTGTTAAAAACAATTTCTCTTAAAATCCTATGAACAACTAAATCTGGATAACGTCGAATTGGACTAGTAAAGTGACAATAGTGTTCACTTGCTAAACCAAAGTGGCCAATATTATTTGGAGAATATTTAGCTTTTTGCATTGTTCTTAAGAAAACTGTTTTTAAAAAGTCATCATTTCTTGTTTCTTTAATTTTGTTGATTATTTTTTGAAAACTTTTTGGATTAATATCATTTCGATCAATGTTTACTTTAATACCTAAAACATTAAGTACACTCAAAAATGAATCAATTTTGTCACTATCAGGGCTTTCATGAACACGATAAATAAAAGGTATTTTTCTTTTAGCTAAAAATTTAGCTACTTCTTCATTAGTTCTAACCATGAAATCTTCAATAAGATTTTCACTAAAACCAGTTGGCTTAACTACAACATCAATTGTTTTACCTTTATCATCTAAGACAATTTCGGGTTCTTCAATTTCAAAATCAATGTAGCCTTCTTTGACTTTATAATTTCTAATTATTTTTGCCAATTCAGCTGCTTCATAGAGCATTCGATTTAATTTCGGGTCATCAAATTTTTGATTTTTATCATAAAGTTCATTAACCCTATCATATGTGAGACGATATTTTGATTGAATTATTGACTGATAAAATTTAGCATCAATAGTATCACCATTTTTATCAATTGTCATTATTGCACTAAGAGTAAAGCGATCTTCATTAGGATTAAGTGAGCAAATACCATTTGATAATTTATGAGGCAACATTGGAATAACGCCACTAACTAAATATCTTGAGGTTCCTCTTTTTAATGCTTCAACATCAAGAGAGCTGTCTTCTTTGACATAATAACTAACATCAGCAATATGAACGCCTAATTCATAAGTTCCATCAGCATTCTTTTTAACATTAATTGCATCATCAAAGTCTTTTGTGTCATTACCATCAATAGTAACAATTAATTCTTTTCTCAAGTCAAGTCTATTATTAAGTTTTTCATCTGCAATACTATTTGGAATTTTAACTAAGTAATCTTTAACTTCTTGTGGGAAATCTTCAGGTTCATTTCTAATAACTGTTAAGGCTTTAACATAAGCCATTGGATCATTACTGTTAGCTATTTTTTTGATAATGTTAAGCTCAATAATATTATTTTTAAATGAAACCATTTGACCTAAAACAACATCATGAAGTCTAGCTTCTTTAACCAATTCTTTAATATGAAAATTAATTTTTTTGTAGTCATTTTTAAGTGGTTTAAATGAAAGAAAACCTTCATGCATACCTAAAATACCAATGAATTTATCACTGTTTCTGCTTAAAACTTTTTCAACAATTGCAAAAGTTTTATCATCTAAAGAATGAAAGTATTTTAAAACTGTTATTTCAACAAAGTCTCCATCTAATGCTCTATTAAGATGAATACCAGGTACAAAATAACTTATTTTTTCAGCTTCAGGATTATCATTTTCTTCTTCTACAAAAGCATACTTGCCTTCTGAAGCAAATCGAATTTTTCCTTGGACGATTTTAATTTTTTCTATTAATGAATAAGTTGAGAGTTTGCCATTTTTAATTAATTTTCTTTCATCAATTAATTTATTTAGTAATTCGGTTAATTTTTTGTTATCTTTATAACTTATTCTTAAGCCTTTTGCAATATCAATGAAAGTTGAATTATCATGTTTTTTTAAAAAATTTAATATTTCTTGCGGATTCATAAGTTATACAATAATCCTTAAAATAATAGCTAAAATTAGCAATGCTATTCCACCAAATAACATTGAGTATTTTAAAACCTTTTTAAGTCCTCTTTCTTTTGAATATTTAAAAAGTTCCAAATCACCACTACCAACAAGTGCACCAGAAAAACCATTACTGTCTGGTGACATAAGCAATGAAATCACAATAATTGCGATCGATAAAATAGCGATAATTATTGATACAAATATAACTGCCATAATTCTCCTTTCAACCTAAAAACTTCTTATTAGTATTAATATTAAGATTATAAAGGAAAAGTTGCAAAATTTATGTTAATTTTTAGCATAAATATTATTATTCTAAATTTTAAGTTAATTTTTCTACTAATTTATTGTATTTTTTATAGTGCTTTTTAATATTTTTAAAATTATTCAATATAATTATGAAGATAAAAAATATATGGAGTTAGTATGAAAAACAAAATAACCAAACACAATATGTGTGAAATTTATGAGTCATTTCGTTTTATTCAACCTGAATATAAAAGTCGTGTAAATTCATTAAAAAATAAAAATGAAAATGACGAATTTTTAATCAAAGCACAAATGGAAAGTGCTATTTCATTGAATGAATATATTGATTCAAAATTAGAAAGAAAAAAGATCAGAATTGCTAATCGTAAGATTTCAAAAATCTCAAGTATCTTTATTCCACTAATTGTTTTCTTCTTAGTAACTGTTTTAATTGCAGTGTCTTATATTGCTTATATAGCAGCTAACTATATGGTTAAAGATGCTGAAGTTTATACACTATTTGGTATAGCTATATTATCACTTATTGCAGCAGCAATATGCTTTGTAGTTTACTATAGAAAATACACGCGATATAACAATGATAAGGCTAAATATTTTGAAGATAAATTAAAAGAAGAAATCAAAATTGGAGATTTAAAATCTATAGTTTTAAAAAACAAAAATACATTAAATTATCAAGAAGATGTTGCTTCATTAGTTGTTGCTAACGATCTAAAATATCCTGATATTCCTGCAGGCTACAAAGTTGAAGCTTCAAAATACGCTAAACGTTTTACACTATATGGAATGAATGTTTTAGCACAAGATGCTCAACTAGTAAAAATTGACGAAAAGGCTAATGAAACAGCTGAATCAGACAAAAGTATTATTAGTAATAAAGCTAAAAAAATTAAGATTTTTGAATTCTCATTTATGAATAGTCAATTTGACAAAATTTATTTACCATTTAACTTATTTAACAAATTTGAAGTTTTCAAAGCTAAACTTGAACCAATCAAAAGTAACGTTTCTGATTTAGACAAAATCTTCAATATTCATGCAATCAAAAACCCGCTTAACAGTAAACTTTTCGATGCTCTTAAATTATGAAAAAATAATATTCATTTTGCTCATACTTTTATACCTCAAGAATCAGGTGTTAATTACTTTAACTTCAAACTTACAGCAATCTTTACTTTAAAAGAAGAATGCGAAGATGAATTTAATATTCAAACAAATGCAAAATACTGAAACAATCCATTCAAAGTTGAATTACCTGAAACAATTGATGATTCAACAAAATTACCAATGTGATTTATTATCAATAATTTCTACAAAATGGTAGACTATATTGCACTTTCAAAAGCAACAGTTGAAAAATATGTTATGCCAATGATATTCTACTTATTTAATGAAATTAATGACACCAAAGTATTTAATGAAAAAACTCTTTCAGATTTATACGAAATTTAATAGATTAAATAGTGCCCTCAAGGGCATTTTTTAATTTTTGCTAATTTCAATATTAAAAATTATTATTAATATTAGTAAAAAAATATATAATGAATATTATGAACAATTTAATTAGCAAACGTAAGCCAATAATAATATTTACAGGACCTAGCGGAGTTGGCAAAGGAACAATTGAAAAGTACCTTTTTGATTTTAAAAATTTAAAATTACATTTATCATGTTCAGCAACAACTAGAGCTCCTCGTGAAGGTGAAATTAATGGCATTCATTACTTTTTTATAACAAAAGAAGAATTTGAAAAACACATTCAAAATGATGATCTTTTTGAATACAGTTTTCATTTTGATAATTATTATGGAACACTTTATAGTGAAATTGCAAGAATTCATAGAAATGGCGAAATTCCTGTTTTAGAAATTGAAACCAATGGTGCAAAACAAATATTAGAAAAGGTTTCAAATAACAAGAATTACAAATTGATCACACTTTTTATCTTACCTCCTTCAATTGAAGAACTTGAAAGAAGAATTATTAACCGAAAGACAGAAAGTCAAGAAGCTATTAAAAAACGTTTAGCTAAAGCGCAAGCAGAAATAGCAGAAAAAGGAATATTTAGACATCACATAATTAATGATGACCCTTTAAGAGCTGCAAAAGAAATAAAAGAAATTATAGAGAAAGAATACGGTATCAAATAATGGAATATGGAATAACAAGCGACATGGGAAGAGTAAGGTTGGAAAACCAAGACAAAGCTAGTTACTTTACAAAAGGTAACTATGTTTTATTAATTTTATGTGATGGAATGGGTGGTCACTATGGTGGTGGGATTGCTTCAAGGACAACAATAAATACTTTTAATAAAATTTTTAACAATTCAATTCCAGCTGAAAATTCTGAAATTAAAGATTATGTATCTTGATTTAAAAAAACTGTTGATGCTTCACGTGATGAAATGATTAAAATTAGTGCTAATGATGAAGCAAAACTAGACATGGGAACAACAGTTACAGCTGCCTTACTTAATACAAAAACAAAATTATTATTAATATTTAATATTGGCGATTCACGTACTTATGCTATGACAACTTTTGGTGAATTAAAGCAAATTTCAATTGATCACAACTTATACAATAAGTTAATTAATGAAGAAGGTAAAAGTCCTTATGAGGCTAAATTGAATCGTTTCCATGCTTCATTAACTTCAGCTTTAGGACCTGATAAAAAAACTAAAATTGAAGTTTTTGACTTAAGTAGTCAATACGAACATGTTTATAGTTTATTAGTCACAAGTGATGGTGTACACGACTTTATCGAAAAACCAAAAATGGAAATGATTTTAAGGGAAAATCTTAATGCTGAAGACATGACTAAAAAATTAGTGGAAGAGGCTTTAGATAATAAGTCAACAGATAACTGCTCAGCAGGTATGGTCATATTAGACAATCTAAAAGAATGGAGTTAAAATGGGAATTAAACCACAAAGTAATATATTTAAGAAATATCGTGTGCTATCAACTATTGGTGATGGTGGTTTTAGTCAAGTTTATAAAGTTGAACTTTTAAATGATAAAAGCCCAATTAAAAGAAAGTTTGCACTTAAATATAGTGTTAATAAAAATAAAAATGATGAAGACATAACTCGTAGAAGATTTGAACAAGAAATCACGATATACAAAAAATTAAATACTGAACGTGTGGCATTATACTTTGACTCTTATAGCGATGAATATGAACAATATCTTGTAATGGAATTTGTTGAAGGACAAAATTTAAGAGAAATAATTAAAAGAAATGGTAAATTCACTACAACAGTTGCTGTTAACTATGCAATTCAAATAGCTGAAGGAATTAGTGAATTACATAACTTAGGTGTAATTCACCGTGATATTAAAAGTAATAACATTTTAATTACCAAAGATAAAAATGTAAAAATTATTGACCTTGGCCTAGCTTTAGATGATGAAAGTCAAAGACTTACACAAGAAAACAAAGTAGTCGGTTCTGTTTTCTATATGGCTCCTGAAATTTGTTTAGCTAATTCGAAACCATCTGTCCGTTCGGACATTTATGCTTTAGGCATTTTGTTATTTGAAATGCTAACTGGCTCATATCCTATTTCAGGTAAAGACCAACAAGAAACTTTAAGAATGCAAAGAACAACAAATGTTCCTAACTTATTAAACTTTGTTAATGCACCTCAAGCTTTAGCTAACGTTATCATCAAAGCAACAGCTAAAGATCCAAACAAAAGATATGCAACTATGTGAGATATGCGTCGTGATCTTATGACTTGTCTTGATACTAAAAGAGTTTATGAAAAACCTCTTGATCTTAGAAAAGTTAAACCTAAAAACACAATTCAAGAAAAAGTTAATAGCAAGAAATTTATTATTGCAGGTATTTCAATAATATTAGTTGTTTTGATTATAGCTTTAGTATTGATTGTTGTTTATGTTCACTAATAAGGAGAGAATTTGAAAGGCAAAATTTATTCAATTAATTCAGGTAAATATTACATAAATGATTTGCAAAATAAAATGCACATATTACCTGCAGCTGGTGTTTTTCGTCACAAAGAAATAACACCTTTAGTGGGTGATATTGTTGAGTTTGAAGAAGGCCAATATATCAAAGAAATATATCCTAGAAAAAATTCTTTTGTTCGTCCAAAAGTAGCAAACATCGACAATATTGTTGTGGTTATGTCAATTAAAGAACCTGAATTTCAAAGTTACTTAGTTGACAAATATTTAGCTTTTATAGAAAGTAAAAATATCAAACCAATTATCATTTTGACTAAAGATGATTTAGGTTCAACCCCTTACTTTGAACAATATAAAAATATGGGTTATGAAATTTACCAAATAAACTATAAAATAAATGATTGAGTAAAAATATTTACCAAAATATTTGATCATAAAACTTGCTCTTTAATGGGTCAAAGCGGAGTTGGAAAAACTACAATAATTAATAAATTAACCAACAATAATTATGAAACTCAAAACATTTCAAAATCAGTAAATCGGGGTAAACATACCACAAGAATTGTTCAAATAATTCCAATATTAAATGGTGAATTAATTGACACTCCAGGCTTTAGCTCTTTTGATTTAAACATCTCAAAATTAGAACTTGCTTCTTCTTATTCTCAATTTAGAGAACTAGGAAAATTATGTAAATTTAAATCATGCTTGCATGAAAATGAACCTGAAGAATATTGCAATATAAAACTCAATGTAAAAAACAAAACAATACCAGAATTTAGATATCAAAATTATCTAAAATTATTAAAGGAGATTAAACATGAATAAAAAAGAAAAATATGTAACACCTTCATTATTAAATGTTGAAAAAGATAAACGTTGTGAAATGGCAACACTATTAGTTAATAATGGAATTAAATGAATTCACTATGATGTTATGGATGGCAAATTTGTACCTAACACAGCTATTGAATTAGATGAAATTAAAAACATTAACAAGAATGCACCTAAACACTTTAAAGATGCTCACTTAATGGTTGAAAATCCTCTTGATTACTTAGATGAATTTAGAGACCATGTTGATATTACAACAATTCATTATGAAGCAGTTAATAAAAAAGAATTGTTGCAATTCTTAAAAGATCATCATTCAGCTTACAGTATAGGACTTGCTATTAAACCAGCAACCGAAGTTAAAGAAATAGTTAAAATGCTTCCTTATTTACAACTTGTTTTAGTTATGTCTGTAGAACCTGGCAAGGGAGGACAAAAATTTATTGATCATGCTGTTGATAAAATTAGAGAACTTAAACAATTAAGAGACAAAAATGGTTACAAATATTTAATTCAAGTTGATGGTGGAATTAATGGTGAAACTGGACCAAGATGTTTTGCAGCTGGAGTAGATGCAACAGTAGCTGGAACTTATATTGTTTCAGAACCAACTAAGGAAAGAATTGCTAGTGTATTTGGTAAAAAATAATGGTTGCTTTATTGCAACTTTTTTTATACAAATTAAGCAAAAAAGGAATAAAAAAATATGTTGACTAAAACAACATATAATTTTTATTATTTTGCCTTATTATAGGCTTTTGGTAAATTAGGCAACAACACTAACACCTTTGTTCTTTAAGGTTTTTGCTGTTTTAGCACTAACTCTTAAAGTTACTTTTTTACCATTAACATTCATTGTTACTTTTTGCAAGTTAACGTTAAATTTTCTTTTTGAAGCATTCATTGCGTGTGAACGAATGTTTCCAACCATAGGTCCTTTGCCTGTTAATTGGTCTCTTCTTGACATAAGTATTGCTCCTTTCCTAATTTACGATATTCGTTAAATGTATACAAGTAAGTTAATTATACATTATATGTATTTTTATAAAAATGTTTTTTTAATTATTTTTTAGCCTATTTTTTTCTTGCTTGAGCTCTTCTATATCTTGTAATATTGTCAACAATAATGCAGAAGAATACATAGTAAAGAATTATAAACAATCAGCCAATTACAATTCCAATTCAGAAGAAAATTTCAAAAACAATTGAAAATGCTCTTACTGTTTCTCAACTAATTGTACTTGAATTATTATTATGAACAATAGCTACCATTACAAAATACAAAATTGCTGTAACTGCTGCAACAGCATAACAAATTAAAGCTACTAAAACAAAAATTTGTGATGCTTTTGTGTGTCTAAAACGACTAAAATCTTCTGTACGTTTTGCCATTATTCTATTGTAAAGCAAGTAAATAATAAGAACAAAGACAAAGGCTATAATTGCTAAGATAAATGCTGCTATTGACATTCCTAAGGATGTTCTTACTCCTATAACTACACCACTTAAAGTAGCGAATGCAATTCTTGAAACTCCAAATAATGCCATAGCTATAATAGTCATTAATGAAGCAAGAGCAAACATTACTGTTAATGTTATAAATCTTTCTTTTAAAGTTCTAAACATATTGTCTCCTATTTAATCTTTTAATGTATCAGAAATGTGTTTTGCAGCTATTGCACCATCGGCAGCTGCTGTAATAATTTGTCTTACTTTTTTATCTCTGATATCACCAATTGCATAGATGCCTTTTACTTTTGTTTCCATATCTTCATTGGTTTTTACAAAACCTTCTTTAGTTGTTATTTTTAGATGCTTAAATGCATCTTGGTTTGGTATAAAGCCTATGAAAACAAATAAGCCATCAGCTACAATATTAATTTCTTTATTGGTTTTTTTATTCCTTATAGTTACTGAAGTTAACTGATTTTCACCATTTAAAGAAATTAATTCATGATCATAATAAATTTTAGCATTTTTTTGCTTTTTGAGTTCCTCAACCAATTCTTCCTCAGCACTAAATGTATTTTGATTTGTAACTATTGTTACATCAGTACATAATTTAGCTAAAAAAGTTCCTTCTTCAACTGCAGAATTTCCGCCGCCTAATACAACTACTTTTCTATTCTTATATAATGCACCATCGCAAATAGCACAAAAGCTTACATATTCTTCAAGTCTTTGATAATTATCAACACCTTTAGGATATCTATTAATCATACCTGAAGCTATAATTAATTTTTTAGCTTGAATAATTTCACCATTTTCAAGAATTGCTTCTTGTAAATTTTCATTAATTGAATTGATTTTTACAACCTTGCCATAAAGATGTTTTGCGCCATATTTTAAGGCATGTTCGTAAAATTTTTGTGATAAGTCAAAACCACTTATATCTTGAATTCCAAGGTAGTTATCTACCTTAGCTGTTTTTTGAATTTTGCCACCAAAGACATCATTATCAATAAAAGCTGTATTTAGTCCTGCTCGAGAGGTATAAAGAGCAGCATTTAGTCCTGCGGGTCCTGATCCAACAATTAAAACATCAAATTTTTTAATATCTTTTTTGTCCATTATTGCACTAACTCTGTTATTGGCTTACTTTTTTAGTTTTAAAAATTCTTGTTTTTTCAATTCATTGGATATATTTTTCAGGATGAGCATAACCATATTGGAATCTGCCCTTTCTTCAAACACGAACATAGTGAGTTGGATTGAAGAATTCAAAGAAGACAAAAGCTAAACCACCTAAACCAATAAATACAATAGAGGTTATTGTATACATGTGGTATTGGTCTTCACGCAATGGTTCCATTGCTAAACGGACAAGACCATATCACACAAAGTACAATGAAGCACTTGAACCTGGTTTTAATAAACCAAATTGGTTAATAATTCATACTAAAACAATGTAACCAACTAAGTTAGCAAGAGATTCATATAAGAAGAGAGGATAACGATAAGCGCCTTTTGAAATATCATCGCTAATAAACATGTTTTGGGCAAAATTTCTTCCTCAAATTTCACAACTTGCTCCAGTAGGATCTATTTTTCCATAAACCTCATGGTTAGCGTAATTACCTCAACGTCCAATAACTTGACCAATTAGAATTGTTGGAATAATAATTGAAGCAACTTTACGAATGTCAACTAGATCTCTTTTGGTATAAACATAAATCATGTCAAGTACAAAGGCCAAGACAACACCACCTTGGATACTTAAACCACCTTCTCAGATTTTGTATCAATTCTTCCAGTTATAGTTTGGATTATAAATTGCTTCTTGGACTAAGTCTCAAAGACGGGCACCAATAATAGCGGAAGGAATTGTAATTAAAATAAGAGTTAAAAGAATTTCTCATTTATATTTTTCTCTTTGTCAAAAAATAACAATTGTCAAAATAGAAGCCAACATACCTAGCATCATAGTTAATGAATAAACTTGAATTGGTCAATTACCAATATGAAATAATGGTGTATGAGTATTCGCTGGAATTGGCGATAATTCATATTTATAATTCATGTTCTTTTTCTCCTCTCTCTTGTATTTGTTTTAAAACATCGATTCCATCTTTTCTTGCTAAGAAAGTACTTACGGCTGCTTCGATTAAGCTAGCAGCGCTTGAACCATCTTTAACTGGAATTTGAATCTTAGGTAAAGCTCCATCTAAAATTGGAAACTTAAGATCTGAAAGACCTAAGCGATCTAAATTTTTTGCTTCTTCTGTTTGGTTACTTACTAATTCAACAACTAAATCAATTTCACAATTATCAGTTACTGACTTAACTCCATAAGTATATTTCATATCAATCAAACCAATACCTCTAGCTTCCAAAAAGTTTTTAGTAATAGCAGGACTTTTACCATAATAGGTATTACCAACAAGCTTTACTAAAACAACATCATCACTAACAAAAATATGACCTCTCTGAATTAATTCTAGGGTTGCTTCACTTTTACCTGAACCACTTGGTCCAACAATTAAAACTCCAACACCACCGATTAAAACTAAACAACCATGAACTTGAACTTCTTCGGAATATTTTTCACTTAAATATGTTCCAATAGAAGCTGAAATGCTTGATGTAGATGAGTTTTCAGTTGAGAAAACCGGTATTCTATATTTGTTGCAAACCTTTAAAATTCATTTTTTAGGTAACTCTCTAACCCCTTTTGAAAGAATAACTAAAGGCGGTTCTTCTTTAAGGACTCGAGCTAAAATGTTGATAGCTTCTTCTTTACCTTTTTTTGTAAAGAAAATTGATTCACTCGTACCTCAACAAATAATATTTCTTGAATAACGAGTGTTTTCAATTTGACCTGATAATTCTAAACCTAATCTTTTAATCGCAGGTTCTTGAACATCTCGGTAAATAGGATCACATTCTTCATTACAAATTGTTAGTTCGAATTCCTTAATAATTTCTTTAATATTTATTTTTCTTTTTCTCATATATTGTCCTTTTTGAAGCTATTAGCTATTTAATATATGCTTTAATATTTTATATTAATTTTAATATATATTAATTATTTTTTAAAAATGTTACATTTTTGTAAATCCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAATTGTGAACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGACCATTGGGAAATAGACCTTATTATAGGCAAGAGCAAAGGAACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATACGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTAGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAGTGAGACCCCTAGGTTTTAGAGATCTCACTTTTTAAATTGTCCTTTATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGCGAATGAATTTCTAACAATTATTTTAGAAATAAGGTTCACAAAAATCAAATTAATTTGGATACTTCTAAAGATATTTTGAATCATTTAAGAGCTAGAACTTCAATACAAAATATTTCAAAATTTGTTAATTTTAGTACAATGCTATACGTAAATTTATTGACAACTGTGCTTCGGATTTTAGACATTCCAGAAGAGATAAAAAACAAACTTTTGAGATGTGCAAAGTGTAATGAGTAGGGTCATAAAGTAAAGATTATTTCTTTAGAAAGCAAAATTAAAAATCTCAATTCTTACAGATATAAAAGAAATTGTTTTTGTAGCGAAAATATTATAAATAAATAAAAACATTTATTAAGCAACATAAAACTTTATAAATGAATATAATTGCAAAAAAGAAAGCAATGAAATCAAGAAGAAAAATACTATTTTAAAAATCAATTTAAACAATAATAGATAAAATGAATCTAGATGAAAACTGCAATAAATTTATTAGAAAATATATTCCAAAAGGAAAACATTAGACAATTTATCAAACAAATATATTGCATGAATTCAAAATGAAACTAACTCATTCATAAAACATAAAATTAAAGAATATCCATATGCAATGAGTGCAAATAATTTTTCACCTTAAATTATTATGAATAAACAATACGACCTTCAATGATTTTTTAATTTAAAATTTAAGCAGGCGATTTAGAAAAATGTGTTTTTTTAAACTTACCACCCTCGATTTTGAAAATAATAAAAATGTTACGTTTTAGTTTGAAAAAATAAATACTATTTTGAACTAATTCAACTGTTTTATAGTATTTTAAGTAAAATTTTAAAAATAAAATTAGCAATTTTTTGTTAATCAAATTTTGTATCTGGTTTGACTTTATCCATGATTTCTGGTCATTTAAATTCTGACATGTCTCTTAAATTAAAATTTGTAATTATTTCATCATTTGTTGGAAAAGTTAATGCATTTTTTGAATAAACTTTTTGGATTTTTGCTTTGTTTTTTGGATCAAATATTGTATATAAAAACTTCAATTCTTTTTTATAATTTTTCTTTAAACCTATGACTAAATAGAAACCTCTATTTCATACATAATCAAATAATTCACGATCATTTAAAGGACTATAATATTTGTTATTATCAGGATTCAAATAACTTAAAACTAAGCAATTATATCGTTTGAACTCTTCTATATTTTTCTTAATAAAATCTAAGGTAAAAAAGCCTAAACATGGTTTATCATAATGCTTCATTGACATTCAAAATTCATTACTTGAATCATTTTCTAATTTGACATCATACTTTGGTTTATCACTTGTATCTTCTAAAAATTTAGCTGCTTTTTCACTTGGTTTAATATCAAGAAAAGGATTTTGTTTTGATAAAGCTTCAGCTGTTCCGCTGAAGTTTGCTCCTCCTGATTTATAAAAAGGAGTAATGTTAAATTCTTTTAATTTAGAATCATTATTTAAAGTAATATCAGCTAAAGTCAATTGTTCTTGACCACCAAAAGATTCTAATAATATTCTTCAATTATTTTTGTTAAAATAAACATTTAAATCTTTTGCTTCAACTTCTCCACTTGATAATTGAATCTTTGATTTAGGAAGTTTAAGTACAAAAGCAACATTTTCTTTTGCAACTAAAAAAGTCTTAAGTTGATTTTCTAAATATTCTTTTTTAGCTTGTTTTTTCTTTGACGAATTCAAACCAAAACGTGGATCTTTACTTATTGAAGCAAAATCAGTTTTGCCATGAATTAATTGACTTAGGCCAGTGCCACTGCCTCCTCCTACAATATTCATTTCATATTCATAACCTAATTCTTTAGAAATATCATTTAAATACTGAATCATGCTACTTGAACCCTTGAAACTAATAACTGAAAAGTTACAAGAGGTTGTAAAAGTAACTAAGGGTAGGGAATAAATTGGGGCAAGTGCTCGCAAAAGATTTTTCTTTTTTTTCATGTCATTATTATAAATATTTTTCTTTTTTTATTTTATTTTTTCTTGTTTTTGTTAACATAAAGTTAATTGGTGAGACATGCAAAAAACACTGAAGAATGAAAATAAAAATAAATCTTTAAAATATATTTCATTAGTGATGAGCCTTTTAGCTCTTGCTGTTTTAGTTACCTTTGTTGCTTTTATCTTCTTTAAATCATTATTAAGCTGAAAAGAATTTGGTGTAAAAATCTTTTATTTTGATTATAAAATCGCAGGAGTTAAAGGTGGTTTATATTTCCCATTATTAACTAGTTTGATAATTTGCATATTGGTTTTATTTATTAGTTGTCCTATTTCTTTGAGGGTTGCAATCTTAATAATTTATCGAAATAAAAACTATAAAGCTTATCGAATATTTAAATTTATAGCTTTAATTTTAAGTGCTTTGCCTTCTGTTTTATATGGGCTTTTTGGTTTAGTGCCTTTGACTTGAATGAACAAAAATATATTTAATATGGAAAATGGGACAAACTTATTTAATGCCACAATGGTCTTAAGTTTAATGTGCATTCCAACTATGATCAATTCATTATTATTGCAATTTGAAAAAGTTGATTTATGTCTAAAACTCTCAGGTAGTGCTATTGGTTTAAGTGAATCAAAAGTAATTTATAAAATAATTAAAAAGTCATTAAACAAAAAAATATTTTTAATTTATATTAGCTCTTTTGCCAGAGTTATCGGTGAATCAGCTGCTTTAATGTTTATTTTAAATAGCAATAATTATTCAGAAAATTTTACAAATATTAAGAAATTTTTTAACTCTTCATTGCGTCCTCTTTCAGTATTTATTAATGCTAATTTCTTTGCTGAAAATGGTGGCGATGCAACTAGAAGTTACATGTTTAATTTAGCTTTTGTTCTATTAATTGTGATAACTTTAATTAATGCTGCAATCATTATTATTGTTAATTTAAAAGTCAAAAAACCTAAGTGATTAGACAAGTTTAAAACATGACAAGAAATCAAAGATTTCCTGTTGAAAAAAGATAAAAATTATAAAACTATAAATATTTTATATACTATAAACAAGTGGTTTTGGGAAATTGTTGCTTTAACTTTTGTCTTAATATTTTACTCGTGATTTGCTATTGATCTTTTTTATCGAGTCTTAAATTATTTAGCTTTAGGCAAAATTGATGCTGAAACAAATGTGGGCAACTTTAGAGCTTTATTTAACACTTTATATATCTCTTTAGTAGCAATTTTAATAACCTTCCCTTTTGCTTTTTTAGTAACAATTTATTTTGTTGAATTTAACAAAAGCAAAAAAGCAAAATTAGTATTTGATAACTTCATTTCAGTTATGACTTCAATGCCTGCTTTACTTTTTGGTGTCTTTGGTTCAGCTATCTTTTTAGGAACATTTCAATTGGCTTTAGGTGGTAAAACCAGTAACTCACTTTTAGCTGGAGCTTTAGCAATTAGCGTTATGTTATTTGCCTTCAGCTGTCGCTGAATTAAAGAAGCTCTAGAAATAGTTGATCAAAGTGTAGTAACTAGTGCAGCTGCTTTAGGACTCAGCGAAAGTTCAATTTTATTCAAAATAAAATTACCTAAAATTTGGCCTAATCTTTTAAATATTATTATTTTAATGCTGGCTAAAATCTTTTCAGAAACAACACCATTTATTTTAACTAGCGGACTAAGTAATTCAAAACATATTAGCTTATTATTATTTGGCCAAACATTAACAACTAGAATGAATGCTCAATTATCATTACCTTCTGCTTACTCAATTCCAACTATGTACTACTGTGCTATGATGTCATTTGTGACAATAGTTATCCTCTTTTCATTATCAGAATTTGTGATTCCAAAATTAAGCAAATTAAAAAGAAAACATTAGGAGTAAATATGGAAAAAAATACAATTTTTAAATTAGAAAGTGTTAACTTTTGATACAACAAAAAAGGTAAACAAATTCTAAAAAATATCAATCTAGAATTCAAAGAAAACGAAACAGTTGCTTTAATTGGGCCTAGTGGTTGTGGCAAGAGTACAACTATTAAACTTTTGAATCGAATTCATGGGCCACAAGCTGGAAATTATCTAGAAGGTAAAGTTGAATACTTAAATCAAGATCTCTTTAGCAAAAAATTTATTAATGATATTGAATTAAGAAAGCAAGTCGGAATGATATTTCAACAGCCAAGTGTTTTCCCTCTTTCTATTTATGATAATGTTGCTATTGGACTAAGAAATATTGGAATTAGAGATAAAAAAATATTAGACCAAAAAGTGCAAGAAGCTTTAGAAAGAGCTGCACTTTATGATGAAGTTAAAGATAAAATTAAAGAAAATGCAGAAGCATTAAGCGGTGGTCAAAAGCAAAGATTAGCTATTGCTCGAGCAATAGCGCTAAGTCCAAAAGTACTTTTAATGGATGAACCTACTAGTGCGCTAGATCCAATTGCAACTCAAAAAATAGAGAACTTAATTTTAGATTTAAAAAATGATTACACCATTATTTTAGTAACTCACTCAATGGCTCAAGCTCAGAGAGTTAGTGATAAAACTGCATTCTTTTTAGCAGGTGAATTAATTGAAATTAATAGCACTAGAAACATATTTACTAAACCACTTAAAGAAGAAACAAAAGACTACATTATGGGAAAGATAGGATAGAATTATGCCAATAAATTACACTATATTAAAGAAGAGCGAAGATAACTTAAGAAAAATGTTTTTTGATTACTTAGACCAAGCATATCAAATGCATCTTAAATTAACTGAACTTATTTCAATATATCAAAACAAAATTGATATTGAAGGCTTATTTTTAGAAATCAATAATCGTGAAAAACAAAGCGACTTAACTCAAGTAAATACCATTGAAGAATTGCTTTGAGACATTTCAAAAGACCAACCTTTATTAAGCCACTTGCGTTGATATATTTGTGTTTTAACTTCTTCAGGAGACATTGAGAGAATTTGTGATTACCTCTATTTTATGGCCAGATTCTTCTATCAAAACAAAAAAATTCCTCAAGAGGTTGTTAATGCTGCTTTAGCTATGAATATAGAATTAAGCAAAATTTTTAAAGAATATAGTAGCCGTCTTCAAGATGAAAAAAGTACTAAAGAATTCTTCAAAGAATTCCAAAAACAAAAAGTAGAATACAAACAATTCTGTGATAAAGAGATCAAAAAAATGGCTCGTAAATTAACCAAAAATGATGAGTATTCTAACGAAATAATTATTAAGTTTTCTTTTGAATTGCACTCATATTATCGTGTAGTTGAAAGACTAGAAAATATCATTGAAAATTTGATTTTCATTCATGATACAGATTTCTTTAGAAAGAAATTGGAAAATGAAGAAATGTTTTAAAAGACTCTAGAGTGAGCTTTAAATTCCAGTCTTTTTGGAAAGAAATTCATTCTAATTTAATTTTAAATTTTTACTTATTCCAAAAAAGGAATAAGTTTTTTTATGTAACCATTAACATAGGCAACCAAAATTTTTGTACTCTTTTAATACTGCCTTTTTAATATATTGTAAAATATAAATAATTAAATTAAATAATAGGAGGTTAATTATGTCACTTTTTACAAAATTAGTAAATAAAGCTACTAAAGATGTTTTATTGCAAGCAACTTTTAAAGATGCTAAAAAGATTGAATATTTAATCGAAAAAAACAACGAAATTACAGAATATCTTAGTGAAAACAAAGCTTATGTTTACTTAGGAGAAGAAAGCAAGTTAGATTTTAGAACTCTTGAAAGATTTTTTAGATCTTTAAGTGAAAATAGCGCACGTAACTATTGTGTTGATTTAGCTTCATTTGCAACAAAAAATGTTTCAGTTGCTGATGTTGTTGAAGCTTTTACAAGAGCAGTTTACTTTGTTAAAGCTTCTTTATACAATGAAAAAGCTAAAGATAAAGATGCTAAAGAATTTACTTTAGTTCCTTATGTAGAAAAAGTAACTAAAGAAGTTGAAGAAAGATTAGAAAAAACATTAATTTTAGCTGAAGCTACAAATTATGCTCGTAACTTACAAGTTACACCACCAAACATTTGCAACTCAGAATATTTAGCAGACCAAATTGTTAAAGATTTCAAACAATACAAAAACTTAAAAGTTACTGTTTTAAATAAAAAACAAATTCAAGACCTTAAAATGGGATTATTACTTTCAGTTAACAGAGGGTCAGTTTATGAACCACGTGTAGTTGTTATCGAATATGCTGGTGATCCTTCAAGTAAAGAAAAAACAGTTTTAGTCGGTAAAGGTATTACTTTTGACTCAGGTGGTTACTCACTTAAGAGTCCAAGAAACATGTTAGGTATGAAATTCGACATGTCAGGTAGTGTTATTGTTGCTTCAACATTAAAAGCTATCGCACAACTTAAACCTAAGAAAAATGTTTCAGCAATTATGTGTATTACAGACAACAGAGTTAATGGTGATGCTTCATTACCTGACTCAGTTTGAAAAGCAATGAATGGTAAAAGCGTTGAAATCAACAACACAGATGCTGAGGGTCGTTTAGTTATGGCTGATGGTTTAGTTTATGGAGCAACCAAACTTCATGCAACACGTTTAATTGACGTTGCTACATTAACTGGTGCTATTCTTATTGCTTTAGGTCAAACCTACACAGGTATTTGAGCAACAAGCGAAAAAGCTTGAGAAGATATTTCTAAAGCTGCAGACAATGCGCATGAATTAGTATGAAGAATGCCATTTGATGAAGCATTCTCAAAAGGAATCAAAGCTTCTAAAGTTGCTGATCTTAAAAACACAGACTTAAGCGGTTATGGTGGATCAAGCTCAGCTGCTATGTTCTTAAAAGAATTTACAGAAGGCGTTGAATACATCCACTGTGATGTCGCAGGTACAGCAGACATTAATGAAGTGCCACAAGGTGTTTTAGTCAAAACACTTACAGAGCTTGCTTTAATTTAATTTGAATAATAAAGTCCATATCTATTAAATTACTTATTGGACTTTTTATTTACTAACTTGGAGGAAAAAACAATGTATTTAAAACAAAGTGATGGGATTTTAGAAGTTATAACTGGCCCAATGTTCTCAGGTAAAACAGAAGAGCTTATTAAAAGAATTAAAATTTTAGAAATAGCAGATATTAAAACTTTAGTTCTTAAACCTAAATTTGATACTCGCTTTTCTGAAACAGAAATAGTAAGTAGAACAGGTGCAAAAGTTATAGCTAAAAATATTAAAAAATCAAGTGATATAATGAAATTTTGAGACCCTTCTTATAAAGCTGTTGCTATTGATGAAATTAACTTTTTAGATGAAGGCATTCTTGATGTTATTGATGAATTAGTTTTAAAAGGAGTTAGGGTTCTTTGCTCGGGACTTGATATGGATTTCTTAAGAAGACCTTTTGGTGTAACTCCTGCAATCTTAGCAATAGCAGATCACATTACAAAGCTAAAAGCGGTATGCATGAAATGTAAATCTAGTGCAGGTTTTAGTTTTAGAAAAACAAACGAACAATCATTAAATTCGCTTGGTGATGATACTTATGAAGCTCGTTGTAGAGTCTGCCACATCAAAGGTGAACTAAAGAAAAAAGAAGACAAAAAATAAAACAAATTGCAACTCATATAAAGAGCTGCAATTTTTATATATATCTATAAAACAGTTATTTCGGTAAAATTTCTACTTAAATAAATGTTCAAATTTGTCTACTTTAATAATGGCACTATCTAATTGTTTGTCTAATTTTTTATCAGCGCCTTTAACATATTGAAGTCTAAAATAATCCCTTTTAGCAATTCTTTTAGCCTCTTCTCTATTATAGGCATCTCATTCTTCTTGAGTATATTTTTGTCTTAATAAATATGATTTCATGGCTAAATCTCCTTCTTGCTATATGTATCATTATAAAGGCAAAAAGTAGATTTGAAAAACAGATTTTTTACTTCTAAAAACTCTTTTTTAATATATTAAAAAAGAAAATTTATAAATTTTTTTCAATATGTTTTATAAACTCTTTTAATTTAAAAGCTAAGAAATATGGAATGGTATAAATGTCATTTTGAAAACTTATATTGTTTCTTGAAAGTTTAATACCAAAAGCAACATTTTTATTTCTAAACTCACTTAAAAATTTATTTAAAGAAGAACTATCTCCTTTATTAGCTTTTACTTCAATAGGAATAACATTGTTAAAAGTTTCAACAACAAAATCTACTTTAGCTATACCTTTGTTGTTCTTTCAATAAAATAATTTAAAGTCCTGTTTGTATAAACTATTAGCTATTGCATTTTCATACAAGGCGCCATTATATGTATTGAAGTTTTTATTGATTCTTAAATCATTCATACTTTCAACATCTAATTGTGAAATAAGCATACCTGTGTCAAAGTGATATATTTTAAATGAGTTTTCAATAAAGTTTGTTTTAATTGGCTTTTCAATATTTTTTAATTGATAACAGTAATTAATAACTCCAATATGTTTTAATCAGTCTATAGAAGGTATACAGCGTTTGCTTTTTAAATTCAAATTAAGATCATTAAAAACAAATTTCTTGTTTTCACGTCCTAAGAATTTGCCAACCATATCATAAATTCTTTCAACTTTCATCATTTCTTCATAATCTAAATATTGTCCAAAATCATCTTTATAAATATTTTGTAATTCTTTTTGATATTCAAAAACTTTTCCAAAATTACTTTTTGTATTGGGATTAACTAAAATTTATCAATAATATAAGGAAGGCCACCAATAACTAAAAATTCATCATATGATTAAAAACACTTTGCAGCATATTATTTTCAAAAGGTTTTTGTTCTATCAAATGTTTAAAAAGATAATTAATATCTTCATCTTTATATCCTTTTGCTCAAAGGTATTCTTCAAAATCCATAGGTTTCATTTCATACTAAGAAATATAACCGACTCCAATGTGTCTAATATTGTGTTCTTTAAAATTAATTCCTAATAAAAAACCTGAGCAAATAACATCAAATCTTTTGTCTAAGGCAAAACTTTTTAAACTTGTAATGCAATTAGGTTGTTCTTGAATTTCATCAAAAAATATTAAAGTTTCATTTGGCACAAATTCAAAACCGCTTTTTGCAAAAGAAATAAATTTAATTAAATCATCAACTTCATAAGATTTAACATTTTTGTTATTAAACAATTCTTTTATTTCATTCATTAAAGCAAAGTTGATATAAACTACATATTTGTAATTATTTTTAGCAAATTCTAAAATAGAAGTTGTCTTGCCTATTTGTCTTGCTCCTTTAATTATTAATGGTTTTTTATTGGCATCATTTTTTCAATCCAACAAAAAAGCATCTATTTTTCTTTTTAACATAGGACCTACTTAATAATAATTTTAATTATATTTTACTTTTTTCCGTACTTTTTAAACAAAATCTTTACTTTTTCTTAAAAAAGAGCATAAAAAATCCTTAGATTTTGCATCTAAGAATCTTAAATTTAATTAACTTTTTAGTTTTGTATCATAGTAAACTTTGATTTCAGCCATTGTTTGTTTACTTTCTGGTTCAATAGGAAAATGTTTAACTTCATATTTCATTTTTGGTGTTGTATTATCAACATCAAAAATAGTTTGTTCTTTATTTATTCCATAAAGACTAGCAATATATTGTGGATATGTATCATCAACAAAATAGTTATTTTTAACAAATTCATATTGTGATTTTCAAGCAGGAGTATAACCTACAAAGTCAAATGTTGTAAATGATTCATAACCTTCTATATTATTTCATTCTTCAGTAGTACTATTTCATTTTGGACCATTGATTCATTTATCTTTTTCAACTCCACCAAGAAAGCTCTTGTGAACTGTTTTATAAATTAAATCAGTAAAATAATCACTTGTATTTTCTGAGATAACTAACCCATCAACTAATAATAAATTAACTTTTGGTCTTAAGAATCTAATAGTACTTTGAGGCACTGTGGCATAGTTATCTTCTGAGAAATAAGCATCAACTGCATCACCGTTATAAATTAAACCAGCACATGTTTTCGAATCAGGATTAATTAAGTTATTTAAAAGAAATTGTCCATCTCCATTAAAGTTAATATGTTTTGTATCTCTAACACTATAACCTGTACCATGATTAAATAATTCAACAAAGTGGTCAATGTGTTCTTTATATCAATTAGGCTTATCTTTTGATGTGCCTTTTCCAGTAGCGTGATCATCAACAAAGCCTTTATTAACGTCATAAACATAAGCTGAACCATAAATCATATTATCTCTTACAGCATTTGTTGCTTCTCAATGTTCATATTTGTTAGCTTTTAATGTTTTAAGAATATCAATGAGTCTAAATTCACCTTTGTTGTTTTTTATTTCATTAATTAGGTGATTTTGCATTGCTTGTTGATGAGCATTGATTTTATTTTGATATTCAGGGTCATCTTTTTTAAGATTTAAAACTTTAGCAGGATTATAAGCTACTACCATATCTTGTGAAAAGTATGGAGCAAAGTAGTCAATTAAGTGACGATCCTCAGTAGCTTGCGAACCATCGGCATTAATACATTTATCTTTAGTTAAATATTTGTTATATGACTCAAGATGATTTTTTACTGTGTCAGTTAAAAGTTTTAAAACTTCATTGCGGTCATTAAATTTAGGATCACCAAACATTTTAGCATAATCAATTTTGCGAAGTTTACCCTCTCTAATAAGTTGAACTGCTTGAGAATCGTTGCCAATTCCGGCAGCTGCTTTATTAGTTAAAATAGCTTTAGTAAAATCTTGAATTTCAGCAAATTCTTTGAAGTTGAAGTTTTTATTTATTTTTGCTTTGGTATTTGGATCAATATAAGATTGATAGTTGTAGAATGAAGGCTTGAAGTTATTTTTGTTACTATATTTATAAGATAAAGCACCAACTAAAGCAGCTGCAGCAACAACTGCAAGAGCACTTGTACCAAAAGTTTTGGTTAAAATTTTGGTTTTAATAAGTGTTTTAAATTTGTTGTTCTTTGGTTTGTTGTTGTTCATTTTTTATCCTTTTTGCTTTTTTATTACTTCTTTTAATCATGTAAGTTTTATAACCAATATAGCAAGCATTAGCAACAATAGTAACAAATAATAAGCAAGCTCCAACTACTAAGCCTCAAGCTTTAAATTGACCTTCATAAAGTTTGGTTCCAAGTGTACTTACATTTGAAGTTGTTTGTAAAATAATAAAGTCATCAAATGATAAGAAAATGGAAACTAAACCAGCAAAAACAATTGATGGAATCATGTAAACAAAATAAGTTTTGAATCATGATCTAATTTTTGAATAACCTAAATCTTGGCTCGCTTCAAATAATGAAGCATTGAATTTTTCACTTCTTGGATACATTAAAGTAATACCATAAGGTAAAGCCATAATTGTGTGACCTACTATTCCTCTAATCATTCCTTCTTTAGTGTTAGCTAAAGTTCCGAAAAGTGCTGTGAATACTAAAACTAAACCAATAGCAGTAATGTTATCTGGGTTAATAAAGGGTATATTATTAACAGCTCTAACTGCTCTTGAGTAGTTTTTATTTTTTTGTTTTCACATTGCAAACGCTGTCACAAGTGAAATACTAATAACTAATAAAGATACAAAGAAAGCAATAATAATTGAGTTAATTAAAGCTAGTTCTCTACCTTCATCAAAAAAGTTTGTTCAAGCAGTATTAGTAAAACCATTTCATGAAAAGCTCAAGAAACCTTTATCACTTGGCTTATTAAAACTAAAGATAACTGCGAACACTAAAGGAATATAAGTTACAGCTAAAATAATGTAAATGTAACTTCTTTTAATAAAGTTTCAAAATTTAGTCATGTTTCTTACCTCCTTTAGTTTTTTTAAGTCTAATTATAATTTTAGGAACAAAATTGATTAATCCGTAACAACCTATAAATATGGCTGATACAACTAAAACAAGTGTGCTTCCTAAAGCTAATTCATATTTGTTTGAAGGGCTTGAATAATCATTAATTACAGATCCAATAGTTTGTAATTGAGTACCATTTGGAAGTAATTTTGCACTAATAACAAAGGTGGTTGCTGATGATAAGAAGATCATACTGAAACCACTTAAAATAGCTTTGAAACTATATGGAACAACTACTTTAAATAATGTTTGAAATTTGTTGTAACCTAAGTCTGCACTAGCTTCAAGAATGTTTTTAGGCATGTCTTTAAAGACTGTGTAAAGTGGCATAATCATGTATGGCAAGTTTAAATAAGTTAAACCAAAAACCATAAATCATTCAGCATTCAAACTGTCTTCTTCAAAAATTGAAAGGAAGAAACCTCTAATTGCATAAATTTTAGCAATTGTAAAAATTATCATAGGACTAAGAATTAAACTTAAAGCATAAATTGCTAAATATTTGCTCTTAGTTGTAGCAATAAAATAAGCATATGGAAAAGCAATAATTAAACATAATATTGCTGAAATTAATCCAATTTTTAAACTACGTCAAATTATTAATCAAATTCCTTTTTCAGCTAATAAAGCTGATGCATTAAAGCCATCTTGTGGTTTTAAAGCATTAATTACGATTAATAAAATTGGCAAAATTATTAAAAAGATAGCAATAAATAAATAAGGTAATAAAAGTAGTAATCTTTTATTTAAACCAAGTTTGTTTTTAATTTTTGAATTCATTTGTTTTGTAATCCCATTTGCTATCTTTATTCATTAAATGAATTGAGTCAATTGTTCAGCTTAAGTTTACTTTTTCACCTATTTCGAATTTTTTAGCTGTTTCAACATAAATGATTTCATCATTGTCAAGTTGAATTTTTAAGTAGTAATAACTTCCACGGTAAGCCATGTTAATAATTTCACCAACAAGCTTGTCTTTTTTCTTTTCATCATAAGCCATGATATCGATGTCTTCTGGTCTAATTAAAACATCAACTTCAGTTTGGTTTGGAAATTCATCTTCATCATGAATGGTTTTAAATTTACGTTCTCACATTGTAACGCTGCCATCTTCATTAAAGATTCCATTGTAAATATTTGAGTCTCCAACGAATGATGCAACTCATTTATTAACTGGATAGTCATAAATATTTTTTGGCGTGTCATATTGTTCAATTTTTCCACCACGCATAATTGCAATTCTATCTGAAAGTTCAAGAGCTTCATCTTGATCATGTGTAACAAAAATAAATGTAAGACCTAATTTTTGTTGTAAGCTTCTTAATAATATTTGCATTTTTTGTCTAATTTTGGCATCAAGAGCACTAAGAGGTTCATCTAATAAAAGAATAGCAGGTTCAATAACAAGAGATCTGGCTAAAGCAACACGTTGTTTCATACCACCCGAAAGTTCATTAATAGATCTTTTTTCATTACCTTGAAGTCCGACTAATTCTACAATTTCTTTAACTTCTTGGTCCATTTCTTCTTTTGTTATTCTTCTAGTTAAATAACGTTTTTCAAAATTTTCAGTTTGTTGTTCAACATAGTTTTCTCAATATGAGTAATTGAAGTCTGATTTATCTAATCATTTTTGTCTACGACGATATGCAAAAGTATTTGGTTTTAATGTTTCCATTTCTCTTTCATATTTTTCTTGTAGAGCATCTAATTTTTTCATTTCAGCTTGAGCTTTTTCTTCTCATTGTTTTTGTTTTTGAACTAATAATTTTTCATGTTTAGCATTAATAACTTCTTTTGGTTTTCTTTTAAGAGCTAAACCATATTTAATGTTTCCTTCAACATCTAAGTGAGGAAATAATGCATAATCTTGAAAAATAGTTGAAAGGTCTCTTTTGTGAGGCGGCAAGTCTTTAATATCTACACCATGAAATTTAATTTCACCACGTGTTGCTCTTTCAAAACCAGCAATAAGTCTAAGGATTGTAGTTTTGCCAGATCCTGAAGGTCCTAGCAAAGTAACAAATTCACCTTTATTGATTTTTAAATCAACATTGTCCAAAACAACCTTATCATCGAATTCTTTAACTACTTCTTTTAATTCAATAATATAAGGTGTATTATTTTTCCTAATCATAAATTCTCCTAAAAAATAAAATATAAATTTTTTCTAAATAATCTGTTTTATAGCGTTTTTGGTAATAAATAAATTTAAAATATTTTATTTTGGCGTCAAATCAAAGTTAACTAAATTTAATTAAATCGATTGACTTCTTAGGGAGGCTAAATCAAACACGTTTTGAGAATACTCAATAACAAAAAATGGAAGATCTGGATTTTTATCTTCGCAAAAGTTTAATAATACAAATTTAGATTTAAATTTATCACGCTTAAAATTTTGTATTCTCATAATGAGAATAATTATAATAATAAAAAATATTTTTGTTTAAAAATAATAAAAATTTTCATTCTTTGTTTTCTGCATTTTTTATTCATTATTTTCTTTTGTTTTTTTAAAACTAAATAGCATTTCTATTATTACGTTTTTAAGTCTTTAATGATGTTATTTTATTTTTTATAAAAATCTAAGTAAGGAGAAATTATGAGCATTAAAAATAAGAGAGAATATGGTTTGGTTTCATTATTCTTAATACAATTATTTTATTATTAGAATTATCCTTAATAATATTGATTGCAATAAAAGGAAAAATAGGCTTTATGTTATACACCATTTTTGCAATATCTATACCATATGTTGTTTTAGGTTTTTTATATAATGATTTAATACAGGAGTAACATTGTTTATACAATTAATTACAATTGCTTTAGTTATATTTTTAGCATTAAATACAGCAAGTGGGCCAATTTCAAAAGATAGTTCTCATAAGAAAAACAACTCAAATTTTATTTTCACTATTGCCTACTTACTTTTGTCTTTTAATTTTTATATTAGTTAGAGTATTAATATAATTGTCAAAACCTGTGAAATCAAAAATTAATTATTTCAAAAGAAATGATTTTGAAGCAAATGATACAGAATTTGAATCAACTTTTATTGATTTATCAGATAAAAAATAATAACCTAATAATTTTTGTTAATTCCAAAAGTAAGATCTCTAAAAGTTAACTGTTTCACTTTTGGAATTAGGCAACTTAACATCTTTAAAAATAATGTTTTGCATTAATTATATTTAATGTATAATAAACATGATTTTTTCTGTTAGTTATAACTAACAAATTTATGAAAAAAGAGGAGGCTATTATGGCAAATATAAAATCAAAAGTAAAAAGCATTGCTAAGATGGAAGAATTTCGTGTACGTAACAATGCTATGAAAACAAGAGTTCGTAAAGCAATTAGAGTAGCTAGAGAAGCAATTTTAGCTAAAGATAAAAACGCTCAAGAGTTAGTTAATAAAGCTCACTCAATTATTGCTACAGCTGTTCAAAAAGGTGTTTTCCACCCAAATAAAGGTGCAAGAAAATCATCTCGTTTAGACAAATTTGTTAACGAACAAAATGCTAAAAAAGATGCATAATTAGCTTCTGAACTATAAATTAAAAAACCAGAAACTTCTGGTTTTTTGTTTGCTTTAATCATTAAGCTTTATATTTAAGTCCAAGTGCTTTAAGCATTGGCATTAAGAAGAAATTAAATGGTTTGTTAAAGTGAGGCAAGAAGAAGACGTCAGTTAAAGCACATTCAGGAAGAGTCAATTTTCTTTGAAGTGCTAAACCAAACATGTAAATAACTTCTGTATGATTTACTTGACCTCATGATCCGATTTGAACACCTAAAATTTGTAATGTTTTCTTGTCAAAAATAATTTTGCATCCAACTTTTTCACTTTTACCAGCAAATTCGCAGCGGTCATTGTCTTCAAAATATTCACTAGCTACATCAAATCCATGAGCTCTAGCTGCATCTTCATTAAATCCTGTTGCAGCATAGTGACAACCAAAAACATTAATAGCATTTGTTCCTACTACTCCTGGAAATGGCACTTTAAGTCCAGCTAAATTTAATGCAGCTACAATACCTGTTTTAACTGCATTAGTTGCTAAAGCTACGTGTTCATATGTGTTGCTTACACAGTGTTTTAATGCTGCAGAATCACCTAAAACATAGATGTTTTCGTCAGAAATTGATTGTTGGAATTCATTAACTTTAATTGCTCCATTAGGTAATTTTTCAACATCATTCAATACATATGTTCTAGGTTTGAAACCTACTGACATAATTACAAGATCTGCATCATATTTACTTTTATCTGTAATAACTTGTGATACATGTTTTCCATCTTTTGATTTGAATTCAACAACTTTTTCTCCAAGAGCAAGTTTAACACCTTCTTTTTTCATGTTTTTTTGCATGATGCCTGTAAATTCACCATCTAAGTATCCCGGAACAACACGATCAGCTAAGTCAATTAAAGTAACTTTTTTGCCTTTATTGTGAAATGCTTCTACTAATTCAATTCCAATATATCCAGCACCGACAACTACAACATTTTGAATCTTTTTATCATTTGCTTTTTTAACTAAAACTTGAGCATGTTGATATAGTTTTGATAAAAGAATGTTGTCTAATTCAATGCCTGGAATCTTTGATTCAATAGGTCATGTACCACCTGCAAAAACCAATTTATCATATGTGTCTTTAAAGGTTTTACCACTATCTAAATCTTGAACTGTAACTTCTTTTTTCTTTCTATCAATTTCGATAACTTCATGTGAAGTTTTTAAATTTACTCCATATTCTTTTTGCAAAATATTTGGTGAAGAATAAAATAATCCATCTGGTTGTTTAGCTTTTCCACCTACTCAAATTGCAATACCACAACCTAAAAATGATGTGTTTGTATTACGGTCATAAGCTGTAATTTCTGCTTTAGGATTTACAGTTTTTAATGTTCTTAAAAAGCTGGTCCCTGCATGGTTTGCACCAATGACAATAATTTTCATATTTCCTCTTTTCTGTAATAAAAATTTGTATTTGTACATTTTTATTTTATTTAAAAATGTTTTGTTTTAAGTGTAATATGAAAAAAATATTTTTTTACCATAGTTTTATTATTAATAAAAAAACTTGCATTTTTTGCAAGTTAATTTGTATTAAATTTAATGATTATTAAAAGATTTGAGCAACATTTGTTAAGCTAAGTAACACTAATATATCAAGAATTAAGATAATTAACAACACTGCTGCAGCAATACCAACACCTACTCAAATTCCTTTGTCTGATTTTCGTTGAACAGGGTAGAATTGAGTACCATCATACATACCTGTTGGGTATCCATAGTTAGCTTCATATGGAGTTAATTGCCCATAGGCTTCAGGTTGTACTAAATATCCGCCTTCTGTTCCATAAGGATCTGCCATAATTGTTTCACTTTGTACATATGGATTTCCATAAGGGTCCATTGCTGGCTGTGCATTTAGATCAATTGGGTTTCCATATGGATCGTATTGAACTTCAGCATTTGGATCATAAGCTGGTTGTTCTGCTGTTGCAGCTGCTTCTGCTTTAACTTCTTCAATTTTCTTTTCAGCTTCTTCACGTACTGCGTTGATTTCTTGATCTTTTTGGTTTTTAATAGCTTCAATTTCTTCTTCAACTTTCATGAAGTCGAATTTTTTGTTTTCTAATTGTTCTTTTAAGCTATTGATTGTTTCATCTGAAGCATTTTTTTGGTCTTCAAGTTCTTTAATTTTAAGTTCAAGAGATGCTTTGATAGCGTTAAGGTCAACATATGATGATTGACTACGTTTTTTCATTTCAAGATCTGCTGGGAAGTATGTAGCAGGATCTGAAATTAATACAAAGTCAATTTCTGGAATTCTTTTAGCTAAAGCCGATTTGTAAGGTTCTCTTGAAAGATATGATGGGTGAATACTAAATTCTTCACAAATACCGTTATATGTTTCACCACCATCAAAACCTGAAACTTTAGGAATTGATAATAATTTTTTAGAATCTTTGTCTTTTGGATTTTCTGATTCTAATACTGCTAATTGTCCACCAAAAATACCTTCGTTATTTTGAAATCAAATGTAAACTTCATCTCCAAAGTTCAAGAATCAGTCTACAGCATCTTGACGTGTTTTAAATTTTGCAAGCCCTTCTTTAATTTTAGGGTGTTTAAGAAGTCATGGATATGTTTCATCTTCTTTAGGTTTTCATAAAAGATTAAGTCTTTTCATAATTTATTTCCTCGCTTGTCCTAATAATCTATATATTAAGTATTTGAATTATAATTAATTATTTAAATTTTGTACAAATTTTTATAAAAAAAATAGTAAATTCAGCATATTTTGACACTTTTTGCACTAAAAAAGCACTTTCTTTATTGAAAATGCCTTTAATTTTTAAAATCTATTCCAAGTAAATTTAGTCCATTATCAAATACATTTTTGACAGCTTCTAAAATTGGATAGAAGTTTCTATAATCTTTTTTAAGTTTATCTTTTAGATTTTGATTTATCAAATAACACATTTCTTTTAGATATTTTGTTAAGTAAAAAGGATTTAAAGTTTTGATTACTTTTCTTAAAACATAAGGAAAATAATCAAGCCTTAAAATAAGTTTGCTTAAAAAATTGTTTTGATTCATTTTGTTACAATAACATTTATTGTTTTTTGATTCATCTAATAATTTATTTATTTTATTATAAGTATTTAATATGAAATCGAAATTATGACTAATTTTCTTTTGATTTAAAATTTCCTTTTCAATTGTTATTTGATGTTTAGTACTCTTTTCTAAAAAGAAAAATTTGATTTCATTTAAATTAACTTTTTTTACTAATTCATCTATAAAAATGACGTTTTGGTTTTTTTCTGTCATACTTTTAACTTCATTATTTTCTTTAATTTGGAAGTTTTCACAAAAGGCAATATCAATATTATTATTGTAAGCATTTAAGAATTTTAAAGCAGAAATTAAAGAGTGTGAATATTGAGTATGGTTAGCATCTAAAATGTCAATAATTCAGTCTGCTCTTTTTAATTTTTCAAGATGATAAATTATGTCTAATAATAAATATGAACTCGTGCCATTTCTATGAACAAGAATGCGTTCTTTACTATCATCAAGATTTGTATTAAGTATTAAATTTGAATCATTATCATAAATAAAAGGTGACAATAATTCTAATACTTTGTCAATCTTTTCTTTGACCAAAATGTCTTCAAAATAGCTCACTATGTCAAAAAAGACACCTAAATTTTTAAGTTGTTTTTTGGATTTATTTAAGAAATAAAAAACAATTGCTAAATCAAATTTATTTCTTTTAATTGTGTCATTTATGTCTAAATAAAGATCACCATTTTGTGCTTTAATATTCTTTGCACATTCTAAAATATCCTTGTCAACTTTAATAGAATTGTCATTTAATTCAAACAAGTTAACATATTGTTCATGAACAATTTGGCTTACTTTTTCCATTTTCTTTAAACCTGGATAAACTAAAAATTCTCGAGTAACTTGATAACCTAAGTATTCTAAAATTCTTGCTATAGTTTCTCCAACTAAAGCATTTCTAGCATTCATCAAAGTTAAAGAATTAAGTGGCGAAGCTGAAGTATATTCAATATTAACTTTAATATTATTTTTCTTTTCTTGTCCATATTTATAAGCTTTCTTAATAACTTCATTAACCACTGAAACTAAATCTTTTTCATTCAAAACAATATGTAAAAGCCCATAACGATCAATTGTCACATCTTTAACCATTGAATCTTTGATTAATCTCTTTTTAATTTCTTCTGCTAATTCAATATAAGGAATAAAACTAAATTTTTTAAGTTGAAAAACAATATCGCATTCAATATGGTTAATATTATTGTTATGGCCCTTAGTTTTACGAGCCATTTTTAGTGAAAACTTCTTTTCACCATCAAGAAACTTAAAAGAACTGAAAATCTTGTTTTCAGCTTTTAGTTCATTTACTATGTTTTTAATTAACTTGTTCAATTTATGAACTATAACCATATTGCTCCCTGAATATATCTTTAAATATATTTATTTGTATTTAATATTTTAACTTATTTATTATAATTTAACAAATGTTAAAAATTTATGTCTGCGGTCCAACTGTTTATAATGATCTACATATTGGAAATATGGTTCCAATATTAACTTTTGATTTAATGCTAAAAGCTAGACGAGTTTTAGGAGAAAAATTCACTTTTGTTCACAATATTACTGATATTGATGACAAAATAATCAATCGTGCTAGAGAGCTAAATATAAGCGAAAAAGAATTAAGTCAAAAATATACTAAAGACTACTTGAAATTATTAAAAACCTTGGATGTTGACACTATAAGTCATCTTGAAAAAGTTACTGACAATATGGGCTTAATTATTGAATATGTTAATAAGCAAGTTAAAAATAATAATGCTTATATTGACCAAGAAGGTAATGTTTGATTTGATGTTAAAAAGTTTGAAAATCATTATGGTCAAGTAGCAAATTTAAATCTTGACAAAATGATTTTTGAAGAGCAAAATAATAACAAAAAATTTGAAGCAGATTTTGCGCTTTGAAAGAAAACTAATGTTGGAATTCAATTTGATTCACCTTTTTCAAAAGGTCGCCCAGGCTGACATACTGAATGTGTGGTTTTGATTAATAAACACTTTAAAAACAAAGGTTTAGATATTCATGGAGGCGGAATGGATTTAACTTTTCCTCATCATGAAAATGAAAATATTCAACACTTTGCTTTATATAATCAAGACATAACTAAAGAATGAATTCGTTGTGGACAAATTAATTATGAGGGCGAAAAAATGTCAAAATCTTTAGGCAATGTTATTCTTGCAAAAGACTTTTTAAAAGAACATGATCGCGAAATTTTGAAGTTGATTATTTTGAGTTCAAAAATTTCAGCTCCAATTAATGTGACAAATGAATTAATTGAAAATATGTATGTTATTTTGAATAAATACAAAAAATTAATTTTTAAATTCTTTATTAATTTTAAAAATATTGACAAATCAAAAATTAATAAAAATGAAGAATTTATTAAGATAATGAATTCTTTAAAAATAGGCGATTTTTCTGATTTTAATTATTTATTAAATGAACAAATTAAAGAATTCAATAAAACCAAAAATATAAAAAATGCAGAAATAATTTTTAATACTTTAAAAGTTATCCACCCTCAATTAACAATTAAAGCTAACTATAACAAAGCTTTCGAGCTTTTTGACCAATGACGCTGTTTTATAGACAAAAAAGAATATAAACAAGCTGATAAAATTAGAAAAAAATTAATGAAATTAGGAGTTTATTAACATTTTTTGAATTACTATTTTTTAGGAAAAATTGAGGTGCTAAATATGGAAAAATTATATGTGTGTGGTAGAAATTCAGTTGAAGATGCTTTAAAAGTAAACTTGCCAATTGAAACAATTTATGTCAACTCATTTCAACTCGCACAAAAATTAAAACAAATTACCAAAGCAAAAATAGAAATGAAGGATAATTCATTTTTTGCTAACTATCGTAATGATAACCATCAAGGTGTCATTGCTATTTTAAGAGACTTCCCTATTTATGATCTTGAAACAATTAAAAAAGATAATCCTGAAAATGTTTTAGTTTTAGACCACATTCAAGACCCTCATAATTTAGGTGCAATCATTAGAACTGCTAATACTTGCGGAATTAAGCATCTTATTATTAGTAAAGAAAGATCAGCTAATATTACTTCAACAGTTTTAAAAGTTAGCTCAGGTGGTTTTGTTAATATGAAAATAATTAAAGTTAATAGTATTAGTGCTACCTTAACAAAACTTAAAAAATGAAACTATTGAATTTATGTTTCAGCTTTAGATGAAACTGCTCAAAAAGTAAATCAAGTTACTTTTAATAAACCAAGTGTTTTAGTAGTTGGCAATGAAGGAGATGGTGTTAGCAAATCAACTCTAAATGAAGCTGACCAAGTGATTTACATTCCTCAAAAAGGAAGTGTTCAATCAATGAATGTTTCAGTTGCAACTGGTATTTTGTTATATGAATTAACAAAGGACTAACATGAAATATTTACAAAAATTTTTAGACTTACATCAAACACCCACGCAAGAGTTTGATGAATTTTTACATAGCTTAAAAGACAATCAATTGATTATGATATTGGATTATTTTTACAAAAATGAATTTATCAAAAATATCAAAAGCACTTTAATTAGATTTCCTTATATTCCATTAGAAGCTGAAGATATTTATATTGAATTTTTGCAAACCTATTTAGAAGAAGTTAAAAAGTATAATTCAACTGACAAAAATGTTAAATTTTTAAATTTCTTTTTAAATATCTCCAAATTTTACACATTAAATAAAATTAGATATTGGCTTAGAAAAAAAAGAATTCATAACTCACTTATGACTTCAACTGATGAATTGTTATATGTTTTAGATGAGCATTCTGAAGAACAAATTGAACAAAGAATTAATCAAATTGATACTGAAAACTTTTATCATTTATTAACTGACAAAGATAAAAATATAATTAAAATATTGCAAAATAGTATCAATCAAAAGGACAAATTGATTACACCTAGTAAACTAAAAGAATTTAAAACTAAATTTTTAACTAAATTTAATAATTATTTTCATTTTGCCCATTAAAAATGTTTATTAAATATTAATAAAAGAAGCAAAAATAAGTTATAAAATAAAATTTGCTTTTTTTTAATTATTCTTTATAATTTAGATTATGCAAAAAAGAAAAGTGACTCTTTCTTGTGAAAATTGCCAAAAACTTAACTATTCAACAAATAAAAGTTTAAGCAATCCTAAAAGAATAGAGATTAAAAAATTTTGTCCAAACTGTAGAATGCACACAATACACAAAGAGGAAAAATAATGAAAAAGGAAAAAGAAGTCAAAACTAAAAAACCTAAAAAGTATCTATTCAGACGTTTTGTAAAAGAAATGAAAAGAGTTAGATGACCTTCTGTAAGAAAGAATTGAATTTCTTTTGCTCAAATAGTTATTTTTACATTGATATTTACATTGTGTGCTGTTTTACTTGGCGTTCTTTTCTCACTTATTCTTACTAAAGCAGGAGTTAAATAATTGGAGGATATAATGGATAAGAACTTTCAATGATATATGATTAGTACAGTTTCTGGAAAAGAAGATAAGGTTGTTGAAGCTTTAAAAAATAGAATTATTAGTGAACAAGTTGAAGATTGCTTTAATCATGAAGCAACACCTGAAGGCGCTTTTAAAATTTTCAAAAAACCAACCTTAACACCTAAAGAATTAGAAAAACAAAAAAATGGTGAAGAATACAAAGTTAAATATGTAAACATGTATTCAGGCTACATTTTTATTAATATGGACATGACAGATAGAGCTTGATTTATTGTACGTAATACTCAATATGTAACAGGTTTAATTGGATCATCAGGCCGTGGAGCAAAACCTACTCCAGTTTCACAACACCAAATTGATAAAAGTTTTAAAAAAGAAATCAAATTACAAGAAGACTTTGACAAAGGCGTTACACATACAAAATTCCAAATTGGTGAAATTGTTGAAATTATTGATGGACCTTTCAAGGGTGAACAAGGTAGAGTTTTAGAATGTTCAGAAGCAAATAAAAAAGTAACTGTAGAAATCGAATATTTAGGTAAAAAAACTCCAGCTGATTTTGAATACAAAGAAATTAAAAGTCAAGAATATGAATAATTTTTTAACTAGCAAAGAGCTAGTTATTTTTGACTTATTTAACTATAAAACAATTTAATTGATACAAAAGAGCATAATAAAAAAGCCTAATTAATAGACTTTATTTTAACTTTACATTTTGTTAATTTTAGTTCATGAATGTGGTCAATCATCAGGAATTTTTCCTTTGTTAATAACCACCACGCTAGACGTTTCATTTAAAACATTATCATATTCAAAGCCGCCCATTTCGCCCATGTATTTAGCAATATTTACAATATCAGTAAGCTCATCTCTAAAGTTTTCGCCTTTTAATAAAACAAATTTTCCTCTGTTTTTTAATAAGTGTGCTGCACTTAAATATAAAACTTTAATGCTTGCAACAGCTCGAGCAGTAATAATCTCAAAGTAATTTGTTAATTTAAAAAACTCTTCAACTCTTGAATTAATTATATTGACACTTAAATTAAATCGTGCTTTAACTTTTTCTAAAAAATTGCATCTTTTCTTTTGAGATTCAATAATAGTTAAATTGAACTTATTATCTAATGCAATTAGAATTGGAATTGAAGGAAATCCTGCACCGGCACCTACATCTAATATTTTTTTGTTTTCTAGATTTTCATCATAATGCATCATGTAATTTTCAAAAGCTAAAATAGAATCAAGTATACCTTGTTCAAAAATATCATCTAGTGTTTTAAAACCTGTGATATTCATAACTTGATTTTGATCATAAATCATTTGAGCATATTCTTTTAATATGCTAATTTTTGAAGAATATTTTTCAAGTAAATCTTTTTTAGTAATTTTTCTATAGCTCATTATTTATTTGAAGTAATTTCTTTTGCAAATTGTTGATATAAATGAGAAATAGATTTACCACTAATTGATGATTCAATAACATTAGCTAGGAATCTGTCAAGACTTATTACTTCGAGTTTTTTGAATTTACCATCTCTTAATTCTGCATTTTCAATTGAATCAGTAATAATTACTTTATAAACATTTGGATTATTTTCAAAAATTTCAAATCCTTTTGTAAAAATACCGTGAGTTGCAGCTACAATAATTTTCTTTGCACCGTGTGCTTTTAATGTATCAACTGCTTTTAAAATTGTTCCTCCAGTATCAATGATATCATCAATGATAACTGCATTTTGACCTTCAATATCACCAATAAGTCCTAAAACTTCAGTTTGGTTTGTGCCAATACGACGTTTGTCAATAATACAAATTTTTACATCGTTTGAAATTAATTCAGCAAGTTTTCTTGCTCTAATTGTTCCACCGTGGTCAGGTGAAACAACAGTAAATGATTCATGTGTATTTTTAATAGCTTTTGCTAAAGTATAAGCACCACGCAAATCATCAATAGGAATATTGAAGAAACCTTGAATTGCAGGGTTATGTAAATCAACTGCAATAACTTTGTCAGCACCCGCTGTTTGTAATAAGTCTGCTACTAGTTTTGCACCAATAGGTTGACGTCCACTTGCTTTGCGATCTTGACGTGCATATCCATAGTAACTTAAAGCAACTGTAACACTTTTAACACTTGCTCTTTTAAGCGAGTCAATAAATAAGAATAACTCCATTAAGTTTTCATTAACTGGTCTTGATGTTGAAGCAATAACGAAAACATCTTTTTCTCTAACTGTTTTGTCGCTAACTAACATAACTTCGCCATCAGCAAAAGTTGTTTTAGTAATTCCTGTCAAAGGAACTTTAATAATTTTTGAAATTTTTTCTGCTAATTTCAAGCTATTCGGCATACCAAAAAGCATTACATTTTTTTTATCCATTATATAAATTTCTCCTTAAAATGACTTTTATATTTATATTTTAAACTAAAATTAATTAAAATGAATCTTATGGTAAATTATTGTTGAAAATATCATTTTTAAAAGTTTAAATTTTATTTATAAACCTATAAAAAAGGCTTTTAGCTTTATTTTTAGACTGATTATTAGCTATAAATAAATATGCTTTTTAAAAATGAATATTTTTATATAATTATAAACATGGAAAAAAATATGTTCAATTCGTTGTCTGATATTAAAAATACCTATGACAATTTAGTTAAAAAATTGGATGATCATGAAGTTATCAATAACATCAAGGAATACACAAAAATAAGTAAAGAAGTCAACAAAATTAAGGATATAGCAGAAACCTTTACTAAGTACTTAAATTTACAAAATGACTACAATGATGCAAAAGTAATGATCAATTCAAAAAATGAAGAGGAAGTTCTTTTTGCTAAAAGTATCATTGAAGAAAATGAAGTTAAATTAGCACAACTTGAAGAAGAATTAAAAATTTTAATTCTGCCTAAAGATGAAAACGATGACAAGAATGTTATTATGGAAATTAGAGGTGCAGCAGGCGGTGACGAAGCTAATATTTTTGCTGGCGACTTATTCAAAATGTACACAAAATTTGCAGAAGATTTAAATTTCAAATTAAAAGTTATATCTTCTTCAGCAGCTAGTGTTGGTGGTTTTACTCAAATAGTATTTAGTATTAAGGGTGATAAAGCTTATTCAAAACTTAAGTTTGAAAGTGGAGTGCATCGTGTGCAACGCGTACCTGTAACTGAAAGTGCAGGTAGAATTCATACTTCAACAGCAACAGTTACTGTTATGCCTGAAATTGATGATAGTGTTGATATTGAAATCAAACCACAAGATTTACAAATTGATACATATAGATCATCTGGAGCTGGTGGCCAATCAGTTAATACAACAGACTCAGCGGTTAGAATTACACACTTGCCTACTGGTATAGTTGTTTCATCGCAAGATGAAAGAAGTCAAATAGCAAATAGAGAAACTGCTTTAACAGTTCTTAAAAGTAAGCTTTATGATTTAGAAATTCAAAAGAAACAACAAGAAGAAGCAGGGTATAGAAAACTAGCAGGTCATGGTGATCGGAGCGAAAAAATTAGAACATATAACTATCCACAAGATAGAGTTACAGACCATAGAATTGGATATTCAACAAGTTTAAAACAAGTAATGGAAGGTAAATTATTAACTATTATTGAAGCATTACTAACAGAAGAACAAAATCAAAAAATAAAGGAAAGTGGCTTATAAAATGAATAACGAAGAAACATTAATTTTAGAAAAAAAGCGGTATGGTTTGGAACCTAAAATTACTGAAAAAGAATATGAATTATTAAGGCAAAATTATCCAATTCAATTAATAATGGGCTATGTTGAATACTTAAATACTAGAATTAATTTAAGAGAACAAGTTTTAATTCCAAGATATGAAACTGAAGAAATGGTTGATATTTATCTAAAAAAATTTGCAGCTGAAAATCAAGAAGTTTTAGATCTTTGTTGTGGTTCAGGTTTTATAGGTTTAGCAATTAAGAAAAACTTACGTTCAGCTAAAGTAACTTTAGTAGATATTAGTGATGAAGCTATTAAACAAACCATTGAAAATTCTATAGTAAATTTTGGTTTTAATCATGACTTAAAAATTTACCAAAGTGACTTGTTTACTAATGTTAAGGGAAGATTTGATGTTATCATTTCAAATCCACCATATTTAAATTGCAATGACAAAAGTTTAGACTGAAAAGCATTAAGTTTTGAACCTAAAATTGCATTATGTGCACCTGAAGATGGCTGATATTTTTATGATAAAATCTTAAAAAAATACAAGAATTATTTAAAGAAAAATGGTTGATTAATTATGGAAATTAATCCTCAACACATTGAAAAATGAAAACAAATTAAGGGAGCACAAATTAAAAAAGATATCAATGGTAAATATCGCTTTGTATTTATTCAAAATATTTAGCTTTTTTAATTTATAATAAGAACATGGAAAAAAATAAAATTAAAGAAATTGTTATTGCAAGATTAGACAAACTTTCAAAGAAAAAGGTAACTTTTACTTCAACACTCGAAGAATTAAAAATTGATTCATTATCACTTGCTGAATTAATTTACGAAAGTGAAATAGAATTCAAAATTAGAATTGAAGATGAAGTGCTTTTAAAAATTAAAAATGTTAATGATATTATTGATGCAATAGAAAATGCTAAGAAAATTTAATTTTTGTGTATAATTATTTTTAGCTTATTAATAGGAGTATAGTTCAATGGTAGAACAACGGGCTTCAACCCCGTGTGTTGTGGGTTCGAGTCCTGCTACTCCTGCCATATCAGAATGACCTTTTTGCAACATTTGTTGCTTTTTTTATACAAAAAATCTAACCAAATGGTTAGACTTTTTTGATTATTATTTATTTTGGAATGATATTACTAATTTTTGATTTTTTCCTTTTATACGGAAAATAACTTCATATTCATCATACGTTTTTCTTACTGCATCAATTAAAACTAAATTATCAAGCACTTGTTGTTTAAGATGAGGATTACTTTCAATTTCAACATTTTTAAATTTTATAAATGATTTTATTTTAACTATTAATTCATTTAATTTTTCATGAGAATCAATTGAATAAAAATCTGTTTTATCTTTAATTAATGCAAATAATTCTTTTTGTTGAGCAACTGTAAAATTTTCAATTTCAGCTCAAATACCTTTAAGTTGTGAAGCTACTGGGCTTAGAAACTTATCTATATCAAAATCTAAATTAATTCCATCGGTTACATAAGGTAAACTATATCCATACATTTCTCTCATTTTGGTATCTATTACTGATATTTGAAGATTATTTAAACTATGACTAATAATTCTAACTTTAAATCTGTTGCCTTTATCTGTCGTTTTTGTATAATCAATTTCAAATACTTTTTCAAGATCTCTATCATGTAAATTTTCATCATAATACTTATTATCTTTTAATTTAATGCAATTATTTTTTATAATTTCATCAATAAATTTATTTAACTCATTGTTTGCAAAAACTTTAATTACATTTAAAACTTTCTTGTCAAATTTATTGCTATCAATATTAAATACATGTGTTTCATATCCAAATACCAATTTAACAACAACTTTGTTATTTACTTGATCATATGTAAATTCTACTTTGTCAAATCGATTAAGTCTTTCATAAACTTTAACATTTTCGAAAATTAACATCTTGAATAAATAAGCATTAATAAAGGAAATAGCTGAATCATGTTCTTTTATTTTAGCACCACCAACAGACATTAATTTTTCAAGGAATTCTAGTTTGTCTGCTTTAGATGACATAGCTTGTTCAACACCATATGCACTCATTAAGTTAATAAATTGTGTTTGAATTTCTTGTGAAATACCAATTGTTGCATCTTTTAATTCAGTTGATTTTGTGCTTAAACTTGGACAATGATTTTCTCTAATTTCTGAATCATGTTGATAAACAAATTCAAATTCATCTACAACTTTTCAAGCCTTAGCTTCTATTTTGAATATTTCAACTTGAATTTTAATTTTAGATTGATAGGCATCAATATTTGAGAATTCTTTAATTGTATATCTAAACAATTTTTGATCATCTTTAATCGCTTTGTTCTTTCTAGCTATTTCTGCTTGTTTGAAATATGAATTTAAAAATTTATTAAATTTAGAATCAAATTTATTATTTAAATAATTTTCAATATTTGTAACAATGATCTTTTTTGATTCTTCATCTAACAATATTGAATCATATTCTTTTTGTAGTTCTAAATCAGAAAGTACAAATTTGCTTATATCTTTTTTATTATCAGAAGTTTCTTTTTTAATGAAATCAAGTAAATAAAAGTTTTCTTTAATATTTGCTTCTAAATTGTGTTTAAAATCGTCTGTTTTAACATATTCTTCAAGTTTCGAATCTGTGAATGGAAAACTATTATTAATTTTTGGATCAAAGGTTTTTGGCGAATTAATTGAATTATGTGTTAATTTAATTGCTTCCAAGTCAAAATGATTATAGTCTGGTTCATATAATTCTAAATCTTCAAAAATTATTCTTGTATTTAAATTAAAATTATTTGATGAATCATAATATGTTTCAAATTTACAATTAGGAGCTAATTTAGCATAATTGCCTAAACTTCCACCAGAATCATAAGCATTAATATCTCATTCATTATCATAATAACTTTGAATTTCATTTAAAGCACTTTGATCTGTAAAATCTATTGCTTTAAATTTATTTGATACTGTATAAAAAAGATTACTACCATTCATTCAAGATTTTAATTGACGTCCTAATGATTCATTAAATGTATCAAGACTTTCATTTGGATTAATATAAGCTACCATTGACTTATTCAATCTGTCTACTAATTTAATAAGTGGATTTAAATCTTTAAAGTTATTAAATTGCAAGGTTCTAACTTTACTTATAACTCTTTGATTTTTATTGCTTCCATCTTTTTGATAACTAAATGTTACATCCGCTTCAAATGAATTAACATCTTTTTGACGATAATCAATAATATTTAAATAAACGTTTTCACTTTTTAAATCTGGTCATTCTTTTTTTAATTCAGCAATTGCATTTTTTCATTTTTTTTGTTCAAAAAATCCTATTTGTTTTTGTTTTAATTGAGAAAGAACTTTATGGGCATGATATTGTTGTAAGCCTTCTGGAAGTGAAAAGTCATAATTATCAATTAGTTTGTCCAAATATGCTTTTTCTGTTGTTTTTTCTGTAGCTAAAGTAATTTCTTTTGTGAATTCTTTTGTTGATATTTCATAAAATGACTTTTTACTTTTATCATCTAAATTACGACAAAGTTTAAATTTTAATTCTACTTTATCACCATTAATTGTGTAATCAGTTACAACAAATTTAGTTTTATTATATATAAAAGCTCGTTCCAATATGTTTCAACTATTAAGTGTTGTAATTGGATTAGATAAATCTTTTTTTGCTAATGATTCTAGTGCTGATTTTGTAAGATCATTAATGAGTCAAGAACTATATTTTCCATAAACTTGTGTTTTAAATTCTCCTGTGTAAATATCGAAGTTTTCTGGTTTTAGATAAAACTCTTTTGCTTTGTCAAAAATTGCATCTAATTCTTTAGATATTCTAAATGTTGGTCTTTTTTGTGTAGTGGGCATTGTTGCAAATACTCCGCTTGGTGTAACAGATAAATTTTGAGATTTTTTATTGTATAAAGCAGGTTTAAAACCATCAATAACAGTAACTTTTTTTATTAAACTATTTGTATTTTTATTTCAAAAACTTCAACTAATAATCACAGTTGAAACATTATAAATATTTAAAGTTCAAGATTCTAGGCTTAAATCATAAATAGATGAATCATAATTTTCAAATTTAACTAAATTCGCATTATATTCATAAATAGAAAAGCTACCTTTATCTTCATACGCAACATTTACAGCTTTTGCTGCATTAGCTACATCTATTAAATCAGGATTTTTAACACTATCAAATGGTTTGAAACCTTGAACTTTACAATAACCGTCAACAGAAATATTATTTGCTTTTTCTCTTAATTTAAACGATATATATAAATCGCCTGTTGCAGTATCCACTAGTTTGTTGTCGTAGTTGATATTTATTTCAAATAAGTCCTCATTAAAACCTGTAGCAACAAAATCACTAGCTTCAGTCTTATCTACTAAAACATCTTTTTTAAGAGCTGGATTATAATAATCAATTTTAACTTTTTTTACTTCTTTAAGTAAACTTTCTGAAGGATTATTAGCTGGAACTTTCCCTGGGTTTGAAGTAGTAGCAGATTTTTTGAATCCAGTTATTTCATAGGTATTTTCGTCACTTACAAGATTTGCAGAATTCTTAATTTTAAAGTAAATTTTTACTGAAGTTTCTTGCGCTTCAATTCTTCAATGTATTAATTCATATTTATTTAAATCTAAATTTGATACAACTAAATTTGATATGTTTTTAATATCCTTTGCAAATTTATCTTGTTTATTTTTAACATCAAAAACAATATTTTTTGCAAGTTTATTTAAATCAGCTTTTGTTTCTTCACTAGTTGGTTTACCGGTTGTTCCACCATTATCTTTACCAACACAACTACCTGCTACAAAAGCAAAAGGTAATGTACTTAATGAAGATAGGATTAAGAATAATTTTCTTTTTTTATTTTTCATGATTTCCTCCATTTTTTCTAAAAATCATTTAACTAACTAATTATTAGTTAACTAAATTATAATGTATTTTTGCAAAAAGTTTAAATTTTGTTTAAAAAATAAGTTTTTTTTGCACTTTTTGAATAAATCATTCTTTTAAAGCTTTTTAAAATTTCACTCATTTTTTGGCTCTTTAAGTAATTAATTTCACCTTATAAAAAAATAAGCATATTTTTTAAGAAAAAGTTAAAATTCTTGTTTTAAAATAATCATATATAAACAAAATAAGGAGTTATTATGTCAACACACATCAGCGCAAAAAAAGGTGAAATAGCAAGAATTTGTTTTCTTTCAGGAGACCCTTTAAGATCAAAATTTATGGCTCATAAATATTTAAAAAATGTAAAAGAAGTAAGCTCAGTTAGAAATGAAAATTACTACACAGGAGAATATAGAGGTATCAAAGTTACTTTTGGAGGACATGGAATGGGTATGTTCTCAATTGGAGCTTATGCTCATGAACTTTATAATGAATATGGAGTAGATGTTATTATTCGTTTAGGATCAACTGGTTCATATTATGACGAACACAATGTTATGGATACAGTAATTATTAATAGAGCTTTTAGCGACAACATTTCAATAGCTCAATTAATAAATAAAGAAAATACATATGAATACTTTCCAGACAAAGGTGTAACAGATGAATTAATTAAGGCAGCTAAAAAATTAAACAAAAAAGCACCAGTAGTTTCAGTACACTCAACAGATGTTTTCTATGCAACTAGACCTTTAGAGGAAACTAAGAAAGTATCAAAATGTCAAGTAGTTGAAGCAGAAAGTTATGCGCTTTTTGCAGAAGCTAAAAGAAGCAATAAAAAAGCCGCAACCATTTTACAAGTTAGTGACTCTTTAGCTAAAATGGAATTTAGTGATTATAAAACTAGAGAAACAGGATTTACAGAAATGTTCGAAATAGCTTTGGAAGCATTAAAAAATCTATAATGATTTTTACTAAAAAAACTATAAAAAAGCCGCATAGCGCGGTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAAAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCAACTTTTTTGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAAATGTGGACTGTCAAATTAACAATTGAAAAAATAAAGAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAAGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCCGCTAGAAGACTGGTTGGTGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAATGAAAGAAATGAATTTGGCCATTGGGAAATAGACCTTATTGTAGGTAAGAGTAAAGGAACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATGCGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAAAAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGACTAATGCCCTCACTTTTATTTGTCCTGACGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAAGCCGCATAGCGCGCTTTTTAATTTTTGATTTTTTTAGTGAATTGAACTCCAAAAGCTATTGAATATAGCAATAGTGAAGTGCCTGCTACAATTATGAAATTAACATATCATTGCATTTGATAATCGTTAAATTTGTAAAAACAACCCATTATTTTTTTAAATTGTTCTATGTGTGTTGGATCACTAATTAGACGATCAATAGCAGGTGAAACTAAGGTTTGTCTAAATAAAACATTAGCATGCGAAGCAGGAAAGCATTTAATAATTCATTGAAGCGCATCAGGCAGTGATTCCATTGTTGAATAACCACCAATTATAAAACCTGCTATAGATCCAATTAAAGCATTGATAGCTGCAAAAACATTATGTTTTTTAATGAAGTAAACAATTAAAAAGCTAAAACTACTTCCTATTAAACTTGAAACTAAAATCAATCCAATTACTTCAACAATTTGTAAAAATGATAAGAATTTTGCTCCAAAGCATAATGCATACACAAAACTAATAATAAATGATACAGTTGACATAATTACAGTAACAGCATAAATAGAAATAGCGTTTCCAATAATTATTGAAGATTTATTAACTGGCGAAGCATAAATGTCTTTATAGGCTTTTGATTCTATATCTTCAACCATGAAACTTGAAGCACCTAAACTAACTGTAATACAACCAATTGTCGTTGTAGTTGAAATCATTCATAAATTCATTATTTCTCTAGCGCTTTTGCTTTTATCTTCAATCACTCATCCTTTAAGTCCATCACTAATTGATTTACCTAAAAAAACTGCAACTATAAAGAAGACTAAAATAACACTTAATAAAGAAAAGAAAACATTAGTTTTGTCCATGAAATATTTTTTAATATTTCTTTTAATTATTGCACCCATATTATCTAATCTCCTTTCCAGTTATATTGATAAATGCATCATCCATATCACCCTTTAAAACTTCGAAAGAAGATATCAAATCTTTAAGTTGATTTGCCAAATCTATAGCTTTAAACGAAGAGTCTAAATATACATCAATATAATCTTTAAATTCTTTGAATTTTAAACCTTTGTTTTTTAACAATTCAACTGTTTTAAAGTACTTTACATCATTTTCAAGATATACTTTTAACCTATCTTTTGTATATTCTTCTTTTAGTTTTGCAGGAGTATTCTTAGCCACAATTTTTCCATTATCCATAATCACAACATAGTCAGCTTTTTCAGCTTCTTCCATATAGTGAGTAGTTAAAAATATTGTTGTTTTATTTTTATTTTTTAATTCATAAATTAATTCTCAAATAAGCTTTCTAGTTTGAGGATCTAATCCTGTTGTTGGTTCATCTAAAAATAAAATTTTTGGATAATTTAATAAGGCTCTAGCTATATCCACACGACGTCTTTGACCACCTGAAAGTTTTTCATATTTTCTTTTCAAAATATCTTCAATTTGAGTATCTTTTACAACCCTTTCAAAAGCTGCTTTTATATCTGCTTTTTTACGATAATAATATGCTGCTCTAATTGTTAAATTATCTCTAACACTTAATTTTCGATCTAATACACTATCTTGAAAAACCACACCAATTTGTTTTTTGATTTCATCACTTTGTTTCTTTAAATCAAAACCACAAATTGTTATTTGGCCTTCATTATAATCATTCAATGTACACAAGGTATCAATTGTGGTACTTTTGCCTGCACCATTAGGCCCTAAAAATGCAAAAAGCTCTCCTTCTTTTACAATGAAAGAAATACCTTTGACAGCTTCAACTTTGCCATATGATTTTTTTAAATTTTCAACTTTTATTATTTCAGACATAAAAACCTCCTTAAAATTATAAAGCATATTAATTATAAAAAAATTTTTTATAGTGTGGCATGGTTTTTACATTTTAGAAAACTTTTTTGCGTTTATTTTTAAGGCTTTTAATTTTTAATATATGATTATAAATATATAAGTAATTTAATGTTAATATTAATTATTTGGAAAGGGGTATTATGGCATATAAAGCACTTTATCGAAAATATCGTCCGAAGTCTTTTGATGATGTTAAGGGTCAAGATCATATTGTGCAAACATTAAAAAATATCATTTTAAATCACAAGATAAGTCATGCATACTTATTTAGTGGACCAAGAGGTGTTGGTAAAACTTCAGTTGCTAAAATTTTTGCTAATGTAGTTAACTGTGGTCATGGTGAAGATTTAACTAAACTTTGTGAAAGTTGTATTGAAAAAGCGGATCAAAATTTTGATGTTATCGAAATGGATGCTGCTTCAAATAATGGTGTTAATGAAATTAGAGATTTAAGAGATAAAATCCAACATTCACCAGCAAGTGGAAACTACAAAGTTTATATAATCGATGAAGTTCACATGCTTTCAAAAGGAGCTTTTAATGCACTTTTAAAAACATTAGAAGAACCACCAGCACATGCAATATTTATTTTAGCAACAACTGATCCTCAAAAAATACCTCTAACAATTCTTTCAAGAGTCCAAAGATATAATTTTAGGAAAATATCAAACCAAGTTCTAATTAACCAAATTAAAGAGGTCTTAGATAAAGAAAAAATTAAATATGAAGATTCAACCATAGCTTATATTGCAAGATTAGCAACTGGTGGTATGCGTGATGCACTTTCAATAGTCGACCAAGCTGCAGCTTATGGTAACGGCAAAATATCGCTCAAAGATGTTATGTATGCTTTTGGAATTACTTCAAATGAAAGCTTAATTCAAATTGTAAACTACCTTTATCAAGGCAAAATCAAGGAAGTCTTAATTTTATTTAATAACCTTAAAAATGCGGGAATTGATTCAAATCAATTTGTTCAAAGCTTAATAGAATTGTTCAAGGATTACTTGATTTATGCAAAATCAAATGATGCTAATTTATTAGAACTTTTATCTCTTGAAGAATTAAATACTTTGAAAATTGATGTTAATTTTGCATTCAATTCATCTGAAAAACTTTATAAATTATTCAAAGAATTAATTTTTTCAGAAAACCCATTCCAATTAATCGAACTTTATTTAATTAAACTTAATCAAACTAATGAATTACAAAATAGTAAGGAGCAAACTATGTCAATTGATGTAAACAAAAAGAAACCTAAAAAAGATGACACAATCAAAACAGTTTTAAATCAAACTCAAGAATTAGTACTTGAAGCTAATCGTGACGCTGATGACAATTTTAGCCTAGATCAAGATCTTCTCAGTAGCAATTTTGAAATAGAAGAAGATTATGATGATGGTTTAGTAAGTACAGCAGAAATTCAACTAGATGATGAAATTCAAAATATGCCAAAACCAGTAGTAATTCCTGCTTTTGACTCAAAATACAAAAACTTGAAAACATTCAAATCAAACTTAAGTAAAAAAGATTTAATCAACTTACTTAAACAATCAACATTAACATTTTTAAATAGATACAAATCAAATTTAGATATTGTGATTAACACAGTTCAAAATCCAATTTACAGTGATTTAATTACAGTACTTAAAAAATGTAAAATTGCCGCAGCAGGCCCTAATTACATGATTTTTATCTCAAAAAATGTACCAGAATTAGTTTATTTACAAGAAAATGGTTACAACTCAAATATTCAAAAATTTATTCAAGATTACTTTGAAGATTACAAACACTTATACTGCATTATGAGTGATGATTACAAAGAAACAGCAAAAGATTTTGCAGCTAAAATGAAAGATGCAAAATTTGACACAAGCTTTACAGTACCAGGACCAGTTGTTTTACAAAATGTAAAGAGTCCAACAGGCAAATTATTTGATAAATTCAAAAAATAATTTTTAGTTTATACTAAATTTTATTTATACACTTTTAAGGAGAAATTATGGATCAAGCAATGTTAAGAAAGATGCAAAAATTGCAACAAGAAGTTCAAGAAAAAACTGAACAATTCATGGAAGAAACATTTAAATTAGAAAAACATGGAATTGAAGTTACAGCTAAAGGAAGCAAAAAAATAGTTTCAGTAAAAATCAAAGATTCTATTCTTTTAGACCCAGATGATGCTGAAACTTTAGAAGACTTGTTATCACTTGTGATTAATGAGTTATTTGACACAATTGATGAAGAACAAGAAAAATTAATGCCAGCTATGCCTGGTTTAGGTGGACTTGGCTTCTAATAATGAAAATTGAAACAATTGAAAAACTTAATCAAGTTTTAACAACAATTCCAGGAATTAGTAAAAAGCAAGCAGACAAAATGTCAAACTTTTTCTTAAAACAAGATCAAGAATATATTGACAATTTAGTTAAGTCAATTAATGATCTTAAAACCAACATTTCATATTGTTCTATTTGTAACTTTTTAAAAGAAGGTGGCAAATGTTTAGTATGTGATGATAAGAGCAGATACAACTTTTTAATGATAGTTGAAAATGCTGCAACTTTAAGAAAAATTGATGACATGAATTTTTACAATGGCTACTACTATGTTTTGCCTTATTTGTTTAATTTAAAGAATAATAAGGAAATTAAAAACTATGATTACAAAGAATTAATAAACTATATTCAACATAAAAAATTTGATGAAGTAATCATAGTTTTGAGCCCTTCTTTAGAAGGCGAAATGACAACAGGTCATTTATTAAAATTGATTAATAGCCAATTTAAAATTAAAGTAACAAGAGCAGCTATTGGCATGCCTATGGGTTCAAATTTAGAATATCTTGATACTTTTACAATCAAACAATCTATTGAAAATAGAAACAAATAAAACTTAAGGGAGGTTAATTATGTTTATTACTTTTGAAGGACCAGATGGTAGTGGTAAAACTACAATAATTAACCGCCTTATTATTGAATTAATTGAAAAATATCCAAAGCTTGCATTAAATTATGTTTTGACTCGTGAACCGGGTGGCAAAGACATAATTGAAGCAGAAAAAATTAGATCATTAATTTTGGATAAAGATTCAAAGCTTTCTTCAGTTTCTGAAGCTCTTTTATATACAACTAGTCGTCGAATTCATCTTGAAAGAGTAGTATGACCCGCTCTAAAAGAAAACAGACTTGTTTTGTGCGATCGCTATGTCGACAGCTTTTTTGCATATCAAGGTTATGCTCGTAATTTAGGTATTACTTTTGTTAAAAAACTTACTAATTTAATAATTGATAAAACTATGCCTGATATTACAATCTTTTTTGAAATCAAACCTGAAGAAAGTAAAATCAGAAGAGAACAAATAAGAATTGTTAGTGACAGATTAGACAGTGAAACCGAATTATTCCACCAAAAAGTTTACAAAGGTTATCATGAGCTAATTAAAAAAGAAAAAGATAGATTCATTGTTGTTGATGCTACAAAAAGTATTGAAGAAGTTTTAAAAGAATCATTTGAAAAATTAGATAAACATCCAAGATTTAAAAAATGAATTAAGGAGTTTAAATAATGGTTTCAAAAAATGTCATAACTATTTTAGAAAACTCAATAAAAAATAACAAACTTAGTCACTGTTATTTACTAAAAGCTCCTTCAGGTTTAAACATCGATGATTCAATTTTATACATGATTAATAAACTTTGCAACTCACAATTGCAATCTTTAGATCTTGAAACAATGCTGCCTAATGTTCAAATTTTTGAAGATAATGGCACTGAAAATAATCTTAAAAAAGATAAAATTGTGGCCGGCTTTGAAGAATCAAGTTTATCTTCATTTGTTGAAGGGCAAAAGAAGATAACTGTATTTAAAAATGTTGAAAATGCTTCTAAAGCTGCTCTTAATTCATTACTTAAAATAATTGAAGAACCAAGTGATAATGTAGTCTTTATTTTAACAACTAATAACATAACAAAAGTTTTAGAAACCATCAAATCAAGATCAATAACAATTAACATTAATGCGCCTTCAAACGAAGAGTTAGCAGAAGAATTAAAAGGTGAAAATTTAAAAGAAGAAGCTATATGATTTTATAGCCATATTTTTGCTGATTTAACACAAGTAAAAAAATATACAAATAATGAATCATTTAAACTAATTAAAGACTTGCTTGAAGCTATAGAAAAAAGTATTAAAAATCCTCATTATTTATATATTTTCTTATCTAGATTTACAAAAAAAGATAGCAAAGATACCTTTGTATTTTTGGCTATGGCTTTGCGTTTTGTTTTCTCTTGAGTATGAACGGCTGATGCTTTTTGTAGAAAAAATTATGCTAAATTATTAGTTAAATTAAAAAACATAAAATTTGACTTATTCAACTGTTTTATAGTAATAGACGACTTTTTAAAGATTCAAAATACAAATGCAAATTTCTTCTTACAAGCTGAAAAAATGTTAATTAAATTAGTGGAGTCTTATGCCTAAAATATATATTGTTGGAACACCTATTGGAAACTTAAAAGATATAACTTTAAGAGCTCTTGAAACTCTTAAGGCTGTTGATGTTATTGCTTGCGAAGATACTCGTGTTAGTGCTAAATTATTGAACCATTATGACATTAAAAAACGTTTAATTTCATACAACAAAATCAATGAAAAAGCAAGTGCCCAAGGCCTTATTGATTTAATTGAAAAAGATGACTTAAAAATGGCTTTAATTAGTGATGCAGGTATGCCTTTAGTTAGTGATCCGGGTTTTGAATTAATTAAACAAGCAAGAGAAAAAAATATTGAATTAGAACTAATTCCAGGAGTCAATGCAGCTATTAGTGCTTTTGCTTTTAGTGGACTAAGTAATACCTTTATTTTTATGACTTTTCCTAAAGAAAAAAGTGGCCAAAGATTAGAACAAGTCAAAAGCTTCACAAGTGAACATGCATATGTTTTTTATGTAGCTCCGCATAAATTAGAAAGTTTCTTAAAAGACATTGACTCAGTGTGAGGCAATCAAGCTCAATTATTTATGGCTAGAGAGCTTACTAAAATACATGAAGAATTTATTTATGCAACCGCAACTGAACTATTAGCAAAAAACAAAACTAGTCCTTTTAAAGGTGAGATAACTTTAGTTATCAAACTAAAAGAAATTAAAAAAGAGAAAATAAATAAATATGCTCACTTAAAATAAAATTATTCTTATATAATTAAAGTCAACTTAATTGTTTATTATTTTTCACATTAAATGATTATTATAAAAGGAGATAATATGAATCATATTAATATTTTTGCTCTTGGTGGGCTTGATGAAAATGGTAAAAACTGTTATGTTTTCGAATTCAATGATGACATTTACATTATTAACTCAGGAACTAAAATTCCAATCAACTCAAATAATGGAGTTGATACTTTAATTCCTTCTTTTGAATACTTAGAAAAACACAAAGATAGAATTAAAGGTATCTTTATTTCTGATGTTAAAAATGAATCGTTTAGTGCATTGCCTTGATTATTAATGAAAATTCCTAACTTAACAATTTATACTTCAGCATTCAACAAAATCATGATCATGGATCGTTTATCAAAATATAAGATTCCAACAAAAAACTACAAAGTTATGGTTATTAATAAAGTTACACCATTTAGTGACAAACTTTTTGTTAAGCCAATCGATTTAGCTGGTTCAATGCCAGGACACATTGGTTTTGACTTCATCACACCAGATGGAGATATCTTATTTATGTTTAACTTTGTCGAAGGTGACTTAGGAATTTATGGTAAATTAAGCTTTAATGAACTTAAACAAAGATTTACAAAACGTAAAATTTTAGCTCTAGTTGTTGACTCAGGTAAAGCAAACTTTGGTGGTAAGGCTATTGAAAAAATTGGTTTACCAGAAGGTATTAGAGATGTTTTTTTAGAAACCAAAAAGAATGAAAGAATAATAGTTGGTGCTTATGACGAAGAAATGGTAGCTATCCACAAAATTTTAGAATTAGCTCAAGAAACAAGTCGTCCAGTTGTAACTTATGGTAAAACTTATGGCCAAATATTTTATTTAATTAAAAAAGCTCATCCTGAACTTAAGTTACCAGAATTAATTGATTATCGTAATGCTAACAAAGTTAAAAATGCTGTTATTTTAGTAACTGGTGCTACTGAAAGACTTTACTCAAGATTTATTAGAATAACTGACAATAATGATGTTTTTTTAAAACTTCAAAAAAGCGATACTGTTGTAATGATTGCTCCTCCTATTAATGGACTTGAAAGTCTTGAAGCTTTAACTTTAGATGAAATTGCAAGAATTACTCCAAAAATTAGTGATGTTAATGCTAATGAATTTTATAGATGTCGTCCTGCTAAACAAGACTTAATTGATTTAGTTAATGCTCTAAATCCTGAATATGTGATTCCTGTGCAAGGACTTTATCGTTACTTAAATGAAGCTACTATAGCTATAAGTGAAAATACACAAGTGAAACAAAATCATTGTTTAGTTATGCAAAATGGAAAAATTGTTCACTTTATTGATGGCAAAATGGCAACAACCAAGGGCAAAGTTAAAGAAATTGGCGACACTATTATTGATGGTTTTGGTGTTGGCGATATTAGTACTGAGGTTATTAGTGAGCGTGAAGCTTTAGGTCGTGAAGGTGTGATTTTAGTGTCTTCTCAATATAATCCAAAAACAAAATTATTAACTGGTAAATTACAAATAAATTTTGTTGGTGTCATAAACAAAGAGGAAAAAAATGAAGCTAGCGACATAATCAAAAGCACAATTGTAAAATTAATTGAAACAGAAAGTTTTAATGGAATAAAAGATTTCCAAAATGAAGCTCGTCATGCTATTAGAAAAAGAATTTACAAAACTTTTGAAAAAGAACCAATAGTAATTGTAACATTGGCTCAAATTTAAAATCATAAGTTATAAATTTAGAATTTAAGCAAAAAATAAAAATAATTTATAGAATACTTTTACTAGCCTTATTTATAAGTATAAAAGTATTTTTTTTATATATAAAAAGTTAAACATAAACTTTGTTTTGCAAAAGCATTTTTAATAACATTTTTTAAAATATAAAAGCCTATTTAGCTTAAATATAAATATCTATGTTTTATGCGTTTATGATGTTCTATGACGCTTTTTTTAAATAACCAATAATTTATCTTTTTAGTTGTTAATTCAGTTAAAAATGTGACGCAATTTTCATTTTTAATATAAAAATTAGTAAAAAATTTTAAATTTTTTTAATCTCATATTATTAATAACAAATTTAATATTGCCACATTATAATTTTGCACATATAAACTAAAAAATGAAATCACAAAACAAATTGCAATTTTTTCTTATTTTTTGCAAAATTTGCCAATAAAAACCAATTTTTGAGATATTTAAGCAAAATATCTATATAATGTTCAATATACAATTAAAAATAAGGAGATTATATGAAAAAGTCAAAAAAAATTTTATTAGGATTGAGTCCTATTGCTGCTATTCTTCCTGCAGTAGCTGTTTCTTGTAGAAACAACGATGAATCCAATATTTCATTCAAAGAGAAAGATATTAGTAAATATACCACAACAAATGCTAATGGAAAACAAGTTGTTAAAAACGCTGAATTGTTAAAATTGAAACCAGTTCTTATTACAGATGAAGGTAAAATTGATGATAAATCATTTAACCAATCAGCTTTTGAAGCTTTAAAAGCTATAAATAAACAAACTGGTATTGAAATTAACAGTGTTGAACCTAGCTCAAACTTTGAAAGTGCTTACAACAGTGCACTTTCAGCCGGACACAAAATTTGAGTACTTAATGGCTTCAAACACCAACAATCTATTAAACAATACATTGATGCTCACAGAGAAGAACTTGAAAGAAATCAAATCAAAATCATTGGTATCGACTTTGATATTGAAACAGAGTACAAGTGATTCTACTCATTACAATTCAATATTAAAGAATCTGCATTTACAACAGGCTATGCAATTGCAAGTTGATTAAGTGAACAAGATGAAAGTAAAAGAGTTGTTGCATCATTTGGTGGAGGTGCATTCCCAGGTGTTACAACATTTAACGAAGGTTTTGCAAAAGGTATTCTATACTACAACCAAAAACATAAATCAAGTAAAATTTACCACACATCACCTGTTAAATTAGACTCAGGTTTTACTGCTGGTGAAAAAATGAACACTGTTATTAATAATGTTTTATCTTCAACACCAGCTGATGTTAAATACAACCCACATGTTATCTTATCTGTTGCTGGACCTGCTACATTTGAAACTGTAAAATTAGCAAACAAAGGTCAATATGTAATTGGTGTTGACTCAGACCAAGGCATGATTCAAGACAAAGACAGAATTCTTACATCAGTTCTAAAACACATTAAACAAGCTGTTTATGAAACATTATTAGATCTTATTCTTGAAAAAGAAGAAGGATATAAACCATATGTAGTTAAAGACAAAAAAGCAGACAAAAAATGAAGCCACTTTGGAACTCAAAAAGAAAAATGAATCGGTGTCGCAGAAAACCACTTCTCAAATACAGAAGAACAAGCAAAAATTAATAACAAAATTAAAGAAGCAATTAAAATGTTTAAAGAATTACCAGAAGATTTCGTTAAATATATTAATAGTGACAAAGCTTTAAAAGATGGTAATAAAATTGACAATGTTAGTGAAAGATTAGAAGCAATTATTTCTGCTATTAACAAGGCAGCAAAATAATTAATCAAAAAAATGCTGGAAAATATCCAGCATTCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCAATAGGACAAATAAAAGTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGAAAAGAAGCGTATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTTAAATTTCATGGATTTTTATTAGGAATTTTTACCAAAAACCCATATCTAGAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTTCCTTTGCTCTTGCCTATAATAAGGTCTATTTCCCAATGGTCGAATTCTTTTCTTTCGTTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCGCCAACCAATCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTGACAGTTCACAATTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATCTAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGCTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCTTTGGGTGACTTTTTTTATAAAAAAAACTTCACCCATTATAGATTCTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAATGCTGGAAAATATCCAGCATTTTTTATTTTAAATATGAAAAAAGTATATTTTTTTGTTAATTTTTGAAGAAATTAGATAAAACAGTTTTTCCGTTTTTGTCTTCAAATAAGATAAATAAGAGAAAAAAGGTTGTAAAACTGCCTAAGAAAAAAAGAGTTGGAAAACATAAAAGTTAAAGACCAAATTATTTAACTATTTATGACTTTGAGATGGATACGGTTCATGGGTGTAGAATGGATGAATATTTCATTTTGATTTTAATAAATAGATTGACTAGAGAAGTACACTTTAAAAAAAGATAAAAAAATTCTTTATCTACCAAAATAACTTTATAGGAAATCATATAAGAAAATGGCCTTAAAATCAATACATTAACTATTGATAATGGACCTGAAAATTATAGGCTAGATGAAATCGATGCCGTGGGCCAAATATATCATTGCGATGCTTTTTGTTCTTCATAAAAAGGTCGTGTTGAAAACTACAATAAATTTATTAGAAAATATATTCCAAAAGGAAAAACATTAGACAATTTATCAAATAAATATATTGCGTGAATTCAAAATGAAACTAACTCATTCATAATAGATAAAATTAAAGAATATCCATATGCAATGAGTGCAAATGATTTTGCAATTTAAGTTATGATGAACAAACAATATAACCAACAATGATTTATCAATTTATATCCAAATATTTAAAAATAAAAAATAAATATAAAATTACTAGCTTTCGCTGAATTTTGCTAGCTTGCATTAAAATATTTTCTTTTGCGTTTTTTTCGCTTTCAGTCAAAAAATGACTTTTTAATTTAAAATTTAGCAATCGATTTAGAAAAATGTTTTTCTTAAAACTTACCACACTCACTTTTGGAAATAATAATAAACTATATTTTTAACATATTATTTCTTATTTATCTATTAATTGATATACTTAAATAGTTAATTTTATTATTTATATTAATATATAAAAATATATATAAATATGAGTCTGAAACAAAATTAACTAAAAATTTTATTTAAGAAAAGGACAAAGATGAACGCAATTGAATTTATTAATATTTCGAAAAGTTTCGGTGTAATCAAAGCTAACCAAGATATTAATATTCAAATTAAAAAAGGTACAATTCATGCACTTATTGGTGAAAATGGTGCAGGTAAAAGTACCTTGCTATCAATTCTTTTTGGACTATATGAACCCGACCATGGAATTATCAAGGTTAATGGTAAAGAGGTTTTAATAAATAATCCAAATGATGCTAATAATTTGGGTATTGGTATGGTTCACCAACATTTTAAATTGGTTGGTGCATACACAAACTTACAAAATATAATTCTTGGCGCTGAAAGTGAATCAAAAACTTTTAAAACTTTGGATTATAAACCATCAATTAAAAAAATTAAAACTATTCAAAGATTATTTAATTTAAATTTTGATTTGAATAAAGACACAGGCACTGAATCAGTTTCAACACAGCAAAAAGTTGAAATCATGAAGATGTTATATCGTGATTCAGAAATTCTAATCTTTGATGAACCAACTGCTGTTTTAACAGATGAAGAAATTCAAGGTCTTCTTGAAACATTCAAATTGTTTAAATCGCAAGGTAAAACAATCATTTTTATCTCTCACAAACTAAAAGAAGTTAAAGCTGTAGCTGATTATGCAACAGTTATACGTCACGGTAAAGTTACTGGTAACTTCGAAGTTGCTAATACTTCTATTGAAGAAATGGCTAACAGAATGGTTGGTGACAATATTGTTATGGCACAAAACCAATCACTTGACGTTTTTGAAGATAAAGACACAGTTTTAGAACTTGAAAATGTATCAACAAAAGGTACAAAATCACTTAAAAACTTAAATCTTAAGGTTCATGCTGGAGAAATTCTAGCTATTGCTGGTGTTGAAGGCAATGGACAACAAGATTTAGAATATGTTGTTAGTGGAATTGTTAAACCTACAAAAGGTAATGTCTACTTAAAGAAAACACCTTTAGTTGAGGAAAGAGTTAAATTCATTCATAAAAATAAAATCAAGAATTTCATTATTTTAAGTTCATTGCTTGCATTATTTATAGCTTTTGGATCAATTTTTTTTGGTGTTAGTCCATTTCAGCAAAATTATTCAAACATTTGATTAATGTGAGCTATAGGCATTAGTTTCTTTGCTCTATTTGCTGTTGGTTTAAGTATCTTGATAGTAAAAATTATTAAATTCTTTAAAACCAAAAAAGATGAAGAGAAACTTAAAGACAGGATTAATTTAAACAATTATTCAGTCTTAGAAAAATCAATGCTTGCTTCAAGTTTTATTCCAAGTGATAGACATAAACATGGTCTAGCATTAGATTACAATATTAAAAATAATACAATATTAAGACGTTTATGAGATCCAAAATATCAAACTTTTGGTTTCATAAAACCTAAAGCTGTTAAAAAAGAAACAGCTGAAGTTATTGACAAATATGACGTTCGTGGTGCTAGAGAAGGTTATTCAACTTCTAGATCACTTTCTGGCGGTAACCAACAAAAATTCATTGTTGGTCGTGAAATGAACTCACCACACGACTTTATATTAATTCTTCAACCAACACGTGGACTTGACGTTGGGGCTATTAAAAATATCCATGAAAGAATTCTTAAAGAAAAAGCAGAAGGAAAAGCAATTCTTTTAATTTCTTATGAATTAGACGAGGTTCTTTCACTAGCTGATACTATTGCTGTTATTAACGAAGGCGAAATTAGACTTTCAAAACCCGCAAGAGAATTAACTAGAAAAGAAATCGGTCTTTACATGGCTCAAAAAGAACATCAAACGGAAGGAGGCACACAATAATGAAAGAAACAAAAGAAAAATTATCATTTTCTTCTAAAGTAAGAACAGGCTGAAATAAATTTAGATCATTTATTACACTTGAAGATCAACAAAAAGGAAGAAGAAAATTATACTCATCATTATGAGCTGTTGTTTTCGGATTAGTTGTTGCTAGCATCTTCTTCATGATTCATCAAAGCATGGAAGGACGTAGTGCAGGACCATTCGACTTTGTTATAAAATTATGAAATTATAGCTTTATTGACAAAAACATTAAATTTGACTTTGCAACATTTTTTGTAATATTTGGTTTTACTGGTTTAGCTGTTTCAATCGGTTTTAAAGTTGGATTATTTAACATTGGTATTTCTGGTCAAATGCTTTTACCAGCAATCTTAGTATTCAGTTTTATAGCTAAAGCTAGAAACTTTAATGTTGACGCAGGATTCTTATGATTAGCAATTATTTTGTTCATAATCGTAGGATTTGCAACAGGTGCAATCGCTGGATTATTAAAAGCATATTGCAAAATTCACGAAGTTATTTCTACTATTTTCATTAACTGAATTGTTGCCTTTATATCAGGATGATTATTTAGTTTAAATGGGAAAATGTTTAATCCAGAAGCATATTCACCTACAACTATAGGTAGTGCTAGAATTGCAATTGAAAATGCAGTTAAATATCAATTTGTTTACTTTGGTATTGGAATGTTTGTTTTAACAGCTATTGTTTTATTTGTGATCTACAACTTCACAACATTAGGTTACAAAATAAACATGGTTGGTAAAAATCCTTCAAACGCTAAATATATTGGTCTTAACGAAAAAATGTTAACCATTATAGTTATGGGATTTTCAGGAGCTTTAGCTGGTTTCGCTGGATTCTACTACATTATTATCAAAGAAGGCTGAATTGTCGATTCATTTGGTAGCAACCCTATTGCAATAGGTTTTGAATCAATTGCTGTTTCACTTATTGCATTAAACCACCCTATTGGTATAACATTATCCGGATTTTTCTATTCAATGATTTATACAACAAGAGATTTATTTGCTATAAAAGATAAATTAACAAAAGACTTTTTCCCTATTATTACTGGTATAATTGTCTTTATGGCTGCTTTAGCTATTATGCTTTATAAATTCAAACCACTTGGTTTCATGTGAAAATACGGAACGCTTTGAATCAAAAAAGAATACTGAAAAGAATATGGTTCATACATGAAAACCAAAAATGCTAAATATAGAGAATATGTTAAAGCTCTTAATGAAGAAAAAAGAAGGAATAGAAAACTCAAAAAAGAATTTGCTTCAATTAAAGAAGAATATCAAAAATGAGTTGATTGTAAAATAACTTCACTTAAAAGCGCAAGTGATGATACTATTATGTCTATCTATGACGAAATGTCTAAGAAAAAATTCGAATATCTCAAAAAATACAGTGATTTTGGCTTAAACAATCTTAAAACATTAAAAAATCAATATAAAGCTGAAAAACACGAAAGAAAAATTCAATATATTACTAAAAGAGATTTAATTTATACATCTCATTGAGACAAAGTAAAAGAAAATTGAGCTAAAAAAAGAGAAATGAAAAAAAGCAATCAAGCAAGTAAAGGAGGTGAATAATGTATAGTTGAATATTAATTTTAACTACAACTATTTTTATGTTTTCAGCTCTAACACTTGCAACTCTTTCAGGTATTTTTTCAGAAAGAGTTGGTATTACAAACATTGCTGTTAACGGGTTCATGATTTTTGGAGCTGCTATGTATGCTCTAATAAGCTTCCTAATGACTGATGTAATTTTCAAAGGTAAAACATTAAGTCCATGATTCCAATTATTATACTTTGCTTTTGCGGGTATCTTATCCTCGGGATTTGCACTGTTGTTTGGTTTTGCAACGATTAAACTTAAATCAGATCAATCTGTTTCAGGTTTCGCTATGAACATTCTTGCAGTTGGTGTTGCTTCTATCCTTTTATTATTTGTTAAAAAAGCACAACAAGGTGGAGACTACTTCCAAATGCGTGGCCAAACAGAACTTGCTTTAAGTCCTAATGTTGACAACTGAAAAAACATTATTTCACTTAAACTATTCTTAGCTTTAATTATTGCTGGTGGTTCTTGATTCTTCCTTAACAAAACTAAATGGGGTTTAAGATATAAAGCTATAGGTGAAAATCCTCAAGCAGCAGACGTTGCTGGTATTAACGTTTTTAAATACAAATGATTAGGTATTGCATATTCAGGTTTTATTGCTGGTTTAGCAGGCGCTATATTTATTCAATGTAAAACAAACCAATTCTCACTATACATCGAAGTTGGAGGTTTAGGTTTCTTAGCTCTAGCTATTATGATTACTAGTGGTTGAAAAATTAGTTGAACATTATTGATATGTATCATTTTCTCATTTATCTATGGATTCTCATACTTCGGAATTTCATGACTTGAAAGATTCGAATCTATGAAAGGTGTTTCAAGATTCGCTGAACTACTCTACACATTGCCATTTATTATCACACTTGGAATTATGATTGCTAAAATGAAAAGCAAGCAAATGGGGCCAGCCGCTGCAGGTCGACCTTATGACAAATCAGAGCGTTAGCTATATAAGGAGATTATTATGAAAAAAAGAAAATTTATATTTGCTTTATCATCATTAACAACAGCTTTTTTAGCTGCACCTCTTATTTCTTCATCATGTACTAATGCTGATAAATTGTCTAATCTATTAGATGATTTAGACATTACTATTCCAGATTCAGAATCTAAAAAACCAAATGAAATCAAAGAATCGGATTTAAAATTTTACTCAAAAAATATGCCAAGTTTTAATAGTGAATATACTGTTGAAATACAAAAAATTCAAAAATATTCTTCAGAACTTGTAGTAACTTTTAGACTACGCGAAAAAAACTCAAACATTGTTTCAAAATACAAAACAATTACATACAAAAATTTCTTAAACTTTGATCATATTAATGAAGCTTTAACAGTACAATATTCTAATTCAAAAAATACTTATGTAAAAGATGTTGCTCAAACAAGTGACATTATCTTTAGAGATATTAAAACAGGATACAAAATTATCGATGTTAAAATTGATAACTCAAGAATTGAAGAATATCAAAAAAATTTATCTGACAAACTAAAAGATATAAATGAATTCTTAACAAAAAATAAATCTGAATATGATGCTGAAAAAGCTAAAGCAAATCCTAATAAGGAAATTATTGAAAAATATGAACGCGCTTTAATTAGAAAAGCTAAATATGAAGATACTGCGGATAAAGATTCATATATCAAATCATTCGAGAATTTAAATATAAAATACAAAATTCAATCAATAAATGATCCTAAAATTGTATCTGATGAATTCAGTCGTTCAATTCCAAACTTCAAAAATGATTCATACATTGATTACTATGTTTTTGATAAAAGTGACACATACTCAACAATTTCGCATTCATTTCACTCTGTTTCAGATTTAACTGAAGAAATAAAGAAATACATTAAAAAAACTGCTTCACTAGAATATACAGGAAATACAAATGTTTCTGTATCTGAAGTTAAGGCTGATAAATTTAAATTTAATTTTGACACACCAATTGATTTTTCAATAGAAAACATCAAAGTTGAACCTGCTGATTCAGAAGGCAAAGTAAATCTTAAATTTAATATTAAAGCACCTATTAACAAACAAGCAACAACTTTATTAGTTGATGAAAACAAAAAAACAATTGAATTTGATTATGCATGTGAAATAGAATTTAACAAAAAAGATAGTAAAGAAAAATTAAGTGAAATTGAAAAACAAATTAGCTTTACATATGAAAATGCTGCAACAACAGAAGTTGATAAGGCAGAAGTTTCTAAAGTTAAATACACTTTATCTAACCCAAATTATGAAATTACTGATTTAAAAATTATTGGTTATGAATTCAACAATTCAACTATTGTTATTGAATACAAAGCAAAAAGTAAAAGCAGTTCAATTGAACAAGTTTATTCAAGAACAATTAGTGGCTTTTCTAAACTTTCACAATCTAAAATTGATGCAATTTTAGATTCAATTAAATTGAATTATAAACGTAAAAACTTAGTAGCTGCAAATGAAGCGAATAAAGATCATGTATTTATTGAATCAATGGATTCAAACTTCATGATTGAAGTTACAAAAATAGATTCATTTGATCTTAAAGATAAATCAATATTAGTTGAATTTAAATTATATGAAGTTGATGCACTTTCTAAATTAAAACCAGACAACAAAAAACGCGGTTTCAAATTTGTTAAATCATTCACAAAGAAAATTACAGGCTTCTCAGAAAAATCATTGACACAACATATCAATGAAGAAATTCAAAAGGTCACTTGTACTTGTCCAACAGCTTCAAGCAAATTGCCAAGTCAATTAAACATTAATAATATTAAATTTACTAACTTTGATAACAATATTTTTGAAATTGAAAAACCATCAATAATTGTTGCTAATGATCAAAAAGGCGAAGTTGTTATTACATTTAAATTACGTTACAAAATTGGTTATGTACAAAAAGTTTATGACCAACAAAAGAATGAATATGTTGAAAAAGAAATACCTTTCAAAACTGTTTCAGACTCAAAAACAGTTATATTAAATGGTTTTAAAAAAAATAAATTATAAGGCTCATAAAGCCTTATTTTTTATGCTTTAGGTTTAAAATAAATTTAAAAAACTCAAATATTAAATTTAAAACTTATTTGAAGATTAATATTTGACTTCGAAAAATCAAAAAACAAAAACGAATATTTTCTTTTCAAAACTAATTTTTGCAAAATTTTTTAGACCAATTTTTTTAAAAAAATAGAACACAAAAAAATTTTTAACTAATTTTTAAAATGTGGTAAAAATTACAAAAAGTGCATTTTTGATTAAAAAATAGGTTAAAAAATAATTTTTTATTTTTTAACAAAAGATTAAAAATATGTTAAGATTAAGTTACTTAAAAAAATGAATAAGGAGATTTTATGTTAAAAGAAATTAATCAAGATGAATACAAGAACAATGTTATTGGAAAAGAAAAAGGCATTTATGTTTTGGTTTTTCACGCATTATGATGTCCTCCATGTCGAATGTTTAAATCTTCTTTAGAACAATTAGACAAATTAGACAACATTCCAGTTTATCGTGTAGATGTTGATGAAAATACAAAGCTAACTCAAGAAATGGGTGTTAATTCAATGCCAACATGATTCATTTTTAAAAATGGAAAAATGATGGAAAAAGTTATGGGTTATGTACCTTATGATCACCTTAAAGCAAAAGTTATGGAATACAAATAAAATAATTAAATAGGCTAGTCCTATTTTTTATTTATAATATTTAAACTATGAAAATAATAAGCGACAACAAACGCGGAATGCACAACTATAAAGTAATTGACAAATATGAATCCGGCATATCTTTAATGGGTTGAGAAGTTAAGAGTGCTCGAGCTAATACTGTTAGTTTATTGAATTCATATTGCTTTTTTAGAAAAGGCGAAATTTTCTTGTGCAATGCACAGTTCAAACAATACATGTTAGTTAAGTGTGATGAAACTAGAGATCGCAAATTATTGATGCATAAAAATGAAATAGTTAGATTGCAGTCTAAACTTCATAAGCTAGGTCATGCAACTATTATTCCAAGCAAAATTTATTTTGATAATCGCTCACGTATTAAGATTGAAATAGCTTTAGTTCAAGGAATGAAAAAAACAGATAAACGTGAAGAAATCAAAAAAAGAGATAATGAAAGATACATCAAAAAAAGTCTTGAAAAATGTATATTAAAATGACGCTAAAGTGCGTCATTAATTTTTTAATTATCGAATAAAATTTTTGGTTTATAAATTTTGTTTTCAATTGTGTTATCAACGTCACAACTATAAAGCATTTTTTCAATTTCTAATCCAACTGCAGGATAATCAATAAAGATTTTAGCTTTATATTTGTGTTGATAGTCATAAATTGAAACTCAACCAATATCAGTTAATCTTAAATTACGATCGCCTGATGAAATTAAGTTAATAAATACTTCATGAGTTGAGCAAACAACATTAACCAAATTGTTATTTCTTAAGTAATTTAAGAAAAATTGTACTTCTTTATTATTTTTGTTTTTAAGTGAATAGATTTCATATTCTACATTAAGCTTCTTGCAAGCATTTTCAAAACCTATACAACGAAGTCTTTTTTGTTCTTCAGTTAACTTAACATCTTCAACAAAAACAACCTTTTCGCCTTTATCTATATAGTTTATGAATCTTGAAGTTAAATGATGAAATGAGTTCTCTTCATCAATTTTTATTCAATTTAATGTTTCTACTTCTTTGCCATAAACTAAAATATTTGATTCAAAATTTGTGTTCTTAATATAGTTTGTAATTGTGTTTTGAGGATCTTCAGGAAGAAAGAAAACTATTGCCATAGGTTTTCAGCTTGAAACATATTTGATAGTTTCAATATATTCTTCAGGATTAGGTGAAGAGTGAGTTATAACAACTTTTCTATTTCTTGTCTTTGCTCCTTGTTCAATTCCATTCATAATTTGAGTATATGAGTTTTCATATCACTCAGGCACAATAATAAAGATAGAAGTGTCACGGCCACGAATTGTTCTAGCTCCATGATTTGGATAATAATCGTTTTCTTTTATGATATTAGCAATAACTTTTTTAGTTTCCTTGCTAACATAACCACCACGATAGTAACGGCTTACAGTACTAATAGAGACACCTGCTTCTTTAGCAATATTTTTATATGAGAAATTTTTCATATATTAATTATATAGAAAAAATTAAAATGTGAAAATTTCCACAAAAAAATTTATATTTTTTTTAAATAGTGTATATGAATAAGCAAAAAACGATCTTTAGACCAATAAAAAAAGCCTGACTTAGTCAAACAATTTGAAATAAATAAATTTTTGGTATAATTGTAGCGTCAGTAGAACAATAACCTTGTAACCTGGTCAGGTCAGAGATGAAGCAGCCATATCGAGTAGTGTTGTGTCTATTGACTTTTTTTATTTTTCTAAAGCTACTAATATTTCATATTCTTTAATTAGCTTTTGATAGATCTTTTCTTCTTCATTTTTCAAATGCCTAAATTGTTAATAAATATTTCACTTGGTTTATGTCTTTTCATACTTCAATTTTAAATAAAGTTGACTCAAAATAAAAAATGTTTTGATTTTTAAAAAATGTGTTTATAATATTAAAAGCAAAAACGCGAATGTAATTTAATGGTAAAATGCCAGCTTGCCATGCTGATAATGGGGGTTCGATTCCCCTCGTTCGCTCCATTTATTCTTTATGAATGCCACACCTGTCTTCGACAGGTTTTTTATTTAAAAATCTGTGCAAAAACTAAATCGTTATTTTTTGATTTGACCTTTAACATTATATTTTTTAAATTAAAATTTATATATATTCTTATGTTAAATTAAAAGTTAATAAGTTTATTTTTTAAAATATTTTTTTAATATATTTAAAAAGATAATAAGTATTAATTCTAAAAGTGAGACTTCTAGGTTTTAGAGATCTCACTTTTAGAATTAATAAAACAATGAGAAAATTTATCAAAAATTTTTTGTATATGTAATTATGTTAAATAAAAATACCACAAATGTGGTACTTAAAATAATTATTAAATGTCAATATTCTTTACATATTTAGCGTTTTGTTCAATGAATTCACGACGAGGCAACACTTCATCTCCCATTAATGTTGTAAATGCTCAGTCAGCTCTTGCTGCATCTTCAATTTGAACTTGAAGCATTTTTCTATTAACTGGATCCATAGTTGTTTCTCAAAGTTGTTCAGGGTCCATTTCACCAAGTCCTTTGTAACGTTGAACTGTTACTTTTTGATTTTGATTTAAGCCTTGCATAATTTCTTCTTTTTGAGCATCGTTGTAAGCATATCCAATAAATTTGTTTTGAGCTATTTTATATAAAGGAGGTTGAGCAATATAAATAAATCCGTATTCAATTAAAGGTCTAAAGTAACGGTAGAAGAACGTCAATAACAATGTTCTAATGTGAGCACCATCGACATCGGCATCGGTCATGATGATAATTTTGTGATATCTTAATTTATTAATATTAAATTCTTCACCGACACCTGTTCCAAGTGCTGTAATTAATGAAAGAATTTCAGCATTTGCAAACACTTTTTTAGGTTGATTCTTTTCAGCATTAATAACTTTACCACGAAGAGGCAAGATAGCTTGAGTCATTCTGTTTCTTCCCATTTTAGCTGAACCACCAGCTGAGTTACCTTCGACAATGTAAAGCTCACTAATTTCAGCATTTTTTGAAGAACAGTCAGCTAATTTTCCTGGAAGTGAACCTGTATCAAAAACAGTTTTTCTTCTAGCTGCATCTCTAGCTGCTAAACCAGCAAGACGAGATTTTTTAGCAAAAATTGTTTTTTGAACAATAGTTTTTGCTTCAGCAGGATTTTCATTTAAGAAACGTTCAAATGAATCTGAAAACACTTTGTTAACAGCCATTCTGGCATCTTTGTTTCCTAATTTTCCTTTTGTTTGGCCTTCGAAAATAGGGTTTGTATGTTTAACTGAGATAATAGCAACAATACCTTCTCTAACGTCTTCACGAGTAACTTTGTCTTCTTCAGTTTTGATTAATCCACATTCAGTTCCATAATTATTTACTAAACGAACTAAAGCATCATAAAAACCGCTTTCGTGTGTTCCACCTTCAACTGTAATAATGTTGTTAGCATAAGAAACTACATTTCCTGTAATTCTTTCATTATATTGAACAGCTACTTCAACCTGAACTGCTGGAGAATCTTGGAATGAGTATTCACCTTCAGCATAAATAACATCGGGATGAATTAATTTTTGACCTGTATTTAATTCTTTAACATAATCAACAATGCCACCTTCAAAGTGATATTCTTCTCTTGATTTGTCTCTTTCATCATTAACAATAATTCTTAGGCCTTTGTTAAGATATGCAATTTGTTTAGCATGATCAACAATAACACCTTTATCTCATTCGTTTTTGTCCATAATGCTAAAGTCTGGATGGAATTTAATTCTTGTTCCTGTATCTTGAGGATCTACTTCGTTTAATATTGTAAGATGTTGAAGAATTTTTCCACCATTGTTAAAATGAGCATAGTGTAATTTACCATCACGTTTAACTCAAACTTCAAAGTCATCGCTAAGTCCATTAACTACTGAAGCACCAACCCCGTGAAGTCCACCAGAAACTTTATATGAGTCAGAGTCAAACTTACCGCCAGCATGTAAAACCGTTAAAACTGTTTCAACTGTTGAAAGACCAGTTTTAGGGTGAACTGCTGTAGGAATACCACGACCATTATCGATAACATCAATGTAACCATCCTTTGTAATCGTGATTTCTACTTTGTTTGCAAAACCTGCCATAGCCTCATCAACAGAGTTATCAACTATTTCTCAAATAAGGTGGTGAAGACCTCTAATTCCTGTTGTACCGATGTACATACCAGGTCTAACTCTAACGGCTTCTAATCCTTCTAAAACTTTAATATTCGATGCATCATATTCTTTAGACATATTGCCTCCAATTCTTTATATTTTTTAATATAAAGATTATACCTTAAAAAGGTGAAAATTTTATGGAATTTTTCATAAATTTTTTTTAATTTTATTTTTATAAATTTCAATAAATAAAATTTTTTATTTATTTAATTAAAAGACTATTCTATAGGCTTTTTCGTAAAAAATGACTATTTTAATAAAATTAAAAATATTTAAAAATAACTAAAAAATTGCACTTACAATTATTACAATTAATTAAGGTTTATAAATTTAATAAAAAATAATGTAAAATACTATAAGTGCTTTTGCGCGTTTTTTTAAAATTTTTGAATAATTAAAAGAAAGGAGTTTATGCCTAATAAATCACAAAATGATGAAAAATTAAATCAATCATCAGTCGAAGAACATAATGTAAATGAAGAACATACACAAAAACAAAAAGACAAAGAAGTGATGGACGAGGTTAATGCATTCGAAAGAGACGTTTTACTTAATAATGGTAGAGTCGAAGACAAGCATGTTGAAGAACATCATGAAATGAATTCAATTCATTTAGATGACGTTGTTAACAAAAAAAATACTCAATACAAATACACAAAAATGTCGAATTTTGTGTTGAAATTATCTCGTTTTTATGCTCCTATGCCTCTTTGAAAACTAGGTCTAATCACAGCTGGTTTTGCAATTGTTTTTGGTATTGTCGGAGTTTTCTTTGTTAAAAACCCAGGAATTTATAACTTTGGTTTAGCAGCTTTTGGACAAGCAATTTCAAGATTGACTAACGTTCTTTTAAGATCAAACCCAAACATTAATGACACTATTTATAACGTTATTGACCACGCCTTGTTCTGAATTCTTTATCTTGTTTTGAGTATTCCAATTTTTATATTTGGATGAAAAAAAGTCGGTAAAGTTTTTACTATATTAACACTAGAATTTCTAGTTGTAAGTAGTTTAGTTTCATTTGGTTTAGGTCAAATACCAGGGGCTAACTCAGTTTATATTATTGGTAACTTTTCACACAGTGATATACCAGAAGAATTAAGAAAAGCAGTAGCAGACAAATATTGACAAGGAAAAGAACAATTATGATCACTAGTTCCTCTTCAATGAAATGATGGAGGTAACATAATTGCTCAAATAATTTTTTCTGTTGCTTATGGTTGAATGCTTGCTTTCTTCTTTGCAATTATTGCAATAATAGGAGGTAGTGCAGGAGTTACTGGAATTATTGGCGAATATATGGCTGTCGTTAAACAAAAAAACTTTGGAACAATTAATGGTTATATCAACTTAGTAATTATTATTGTTTCAGTTGTACTTGGTACATACTTACCAGGAAGTTTATTATTAAATGACATTAATGGTTTTAAAGCAGATTCAGTGACTTGAGAAAATACAACAGTAGAAAAAGTTGAAGAAATTCTTAAATCTCTAAAAACGTTACCTTGAAAACCATCGCTTTACTTCTCGCCTAACTTTATATCAACATTTATTTGTAACATTATTTTTGTTATGTACCTTAACAAATTATTCCCTCGTTACAAAATTGTTCAAGTTAAAATTTACTCGCCTCACATGAGTGCTATTAGAGAAGCAATTATTAGTGATAATAAAACTGTTAATAGCTTTACTATCACTAAAGGCGTTGGAGGTTACTCAGGAAATAACACCAAAGTTCTTTCATCAATTACTCTTTACAATCAAGTGCCACGTCTTATTAAGAAAGTTCGGGCCGTTGATCAAAACTCGCTGATTACTATTAGTAATGTTGCTAGTGTTGATGGAAAAATATATCTTCCTGAAAACAAATTCTAAAATTACAGCTGCGGCTGTTTTTTTATTTCAAAAATAAAAGTAATATTTAATATAATAATAAATACTATGAAATTAAACTATATTGAATACAATGGCGACAAAGTTCCTTTATATATAGAAGATAACAAAAAAAATACAACTTTTTTGTTTCTTCATGGACTTAATTCAAACTGAAAATACATGAGACCATTATTAGATTACACACATAATTACAATGTTGTGGCTATTAATTTTCCAGGAAGTAAATTTTTCAAAAATGTAGAACCAGAAGAAATTAAACTTGAGTGATGAATTGAAGTAGCTAAAGAGGTTTTAAATAGAATTAAAACTAAAAAAGTGGTCATAGTAGCTCACTCAATGGCAGGTGGCGTGGCTACTGTTTTAGCTAAGGATCCTAAAGTTGTTAGATTAATTATGATGTCAACAATTAATCCTTATATGAAAGAATCTACTTCTTATTCAGTGTTACAATCTGTTATTAAACCTCAAAATAAATGAAGCTCATTTATTGGCAAAGTTATTTCTAAAATAGCTTCTAAGTTTAAAAAAACTGAAAAATTAATTGAAAGTTTTACTCGACAAGGAGCTTGATTTAACTTACTTGAAAAATATGTTTTGAATCCTGAATATATGGAACAATTAGATTTACAATACAAAGCAAATTGTCACAAAATGCTCTTTTTAGTAGGTGAAAAGGACAAAATTATTGGTACTAAATATTTTGAAGATTATGCAATTAGTTTAGAAGTTCCAATTTTAATTATGGGTACGGGACACTCGCCAATTAAAGATGAACCAAAAAGAATTGCAAGATTTTTTAATGCATTGATTGAAAGCAAAAAAAGACACTTTTGACAAAAATTTATGAATATTAAAATGAATCAATTCTTAGAATTTGATGAAGAAAAAACAAAAGAAGAAGATATTCTTCTTGAACTAGAACAAAACGTTGATTAGGAGATTATTATGGAATACAATCAATTTGAAATGGAACTTAGAAGTGCTTTAACAACAGGCAATGGTACAAAACTTTTATACAAAATAATTGATGCACCTTATAGATTTTTTTCACCTTTGCAACCTTTTAATTACAAAGTTAAATTAGAACAAGCATTCTTAAGAACACAAGAAAACACATATGTAAAATTTATTAAATATTGTGCTGAATATTTAATTACTCAATATGGTTTTAAATTAGAAAAAAGTTCTTTTAAATATACTTTACCACTTAAAGAGGGAGAAGAATTCCCAGAAGAAGTAAAAGTCAATGCAGCTATGATTTTTAGACAAAAAGAAAATGGCGGAGATGAAGAAACAGCTGATATTTGTGTAATTTACTTGAAAAAAAGAGATTACTATGGAGCTAAAGAAGTTACTAAACTTCATGACAATATCATTAAGCAATTAGAAGTATTAAGAATAATTTATCCTAATAATAAAATCAAAGGTATCTTATGATTTTTAGATAATGACTATCACAAAAATGTTAACTTTTTTAATGAATGCTATGATAAAGAACATTCATTTGAAACACCTGGTTTTAGAGCTTATTATGGTGCACAATTTTTCAAAATTTTTGATAAAGAAGAAGACTGATTATTAGTTGAAAATCATATTAAAAAATTTAAAAATAGTAACTATGATTACTTCTTAAAAATGCCAGATTTAGATCAAGATGAAGATGTTTTAAATGCTATGATTAATTTAAATGAAACATCTTGACAAAAATTAATTTCACCTGATGAAGTTTATGCAAATATTAGACAATATATTTTCAATGAAAGAAATGAAAATTCTAACTTATTTAAAGCTCTAAAACTTAGAGATATTAAACGAACTTCAGTAGATGATGATGAAGAAAAAAGACGCATTTTAGAATTGGAAAATAAAGCAAATCAAAATGAATAGATTTTTCTAATCAATCTGTTTTATAGGTAAATTAAACAAAAAGCCAGACACTTCAAATGCCTGGTTTTTCTTATTATTATTTTGTAATTTTTGTTATTCCGTCCATATAAGGAACTAATTTCTTAGGTATAGTAATTGAACCATCTTTGTTTTGATAATTTTCGAGAATTGCTGCAATTAATCTATCAATTGCAAGACCTGAACCATTCATGGTATGAGCATAAACATTTTCACCATTTTCATTCTTATATCTAATCATTGCACGACGTGCTTGGAAATCTCCCATATATGAAACTGATGATATTTCACGATACTTAATTTCTGAAGGCAATCAAACTTCTAAGTCAATAGTTTTTCTACTTGAGAATCCTAAGTCACCTGTACATAATAATAATTCTCTATAAGGTATTTCTAATTCTTCTAAAACCGATTTAGCTTGTTCAAGCATTTGATTAAATTCAGCTTCTCAATCTTTTTCACTAACAATTTTTACTAATTCAACTTTTTTGAATTGGTGGTTTCTAATAATACCTCTTGTATCTTTTCCACTACTTCCTGCTTCTGATCTAAAACATTCAGTAAATGCTGTCATTCTAATAGGTTTTGATAAATCTATTATTTCATTATTATAGTAGTTTGTCAAAGGGACTTCAGCTGTTGGAATTAAATAACTTTGAGCATCTTCTAAAGCAAAAAGATCTTCTTTAAATTTAGGCAATTGACCTGTACCAAAAAGAATTTCAGGTTTAACTATTACAGGTACCGAATATTCTTCATAACCATTTTTGGTATGTAAATCTAACATAAAATTCATAATAGCTCTAGCTAATCTAGCACCTAAACCTTTGTAAATTACAAATCTTGAACCTGACATTTTTACTGCTCTTTCAAGATCGATAATGTCTAAGTCTTTAGCTATAACATAGTGAGGTTGAACGCCTGAAACTAATCCACGTCCTAATTTATCATGATTTTTTATAACTTTATTTTGGTTTTCGTCTTTACCAAGTGGCACATCTTCCATTGAAATATTTGGTATTCTGATTAAAATATCATTAACTTTCTTTTCAATATTTTTAGCAGCTTCTTCAAGTGCTACTTGTTCTTTTTTAATATTTGCTACTTCTTTTTGTAATTTAGCTAATTTAGTTTTATTGTTTTTGTTAGCTGCAAATTCTTTACTAAATTTTGTTAATTCAGCTTTCTTGCTTTGAGCTTCAAACATTAGCTTTCCACGCTTATTTGCTTCTTTAATAAGCTCATTAAATTGCTTTAGATCGAAATTTCTATTTTTTAAAGCTTCTTTAACTTTCTTTTCATTTTCTAAAACGTATTTTAAATTTAGCATTTTGCCTTTCCGCCTATTTTATAGGCTTATAAATCAAAAAATAACCTTAAAAATTCTTCCTTAATTTCATAAAATTATCTTACTTAAATAATACAACAATATTAAATTTTTAATACTTTTTTACAAAAATGATTTAAGACAATAATTTCTTTTGCATTTTTAATAATTAATATATAATTAAGGCACATTTTATTGAGATTTAAGTATTAATATTAATATTTGATAAAATGCATGCATAAAAAAATAATTTAAGGAGGAAATATGAGTTTAAGAACATATCAACCAAACAAGACCCAACACATCAAGACTCATGGTTTTAGAGCAAGAATGGCAACAACAAATGGTAGAAAAGTTTTAGCTGCTAGAAGAGCTAAAGGTAGAGCAAGACTTACAGTTTCAGATGCTAAATAATTAGTTAGTTTATAAAATGAAAAAAATTTATCGTCTTCAAAAAAGTTGAGAATTTGACGCTGTCATCAAATTTCAAAAGCAAGTTCTAAATAAATACATGATTATTTATTACATGCCTAACGATGATTTCAAAATTGGTATAACTGTACCGAAAAAGTTTTGCAATGCAGTTTTAAGAAATTACTATAAACGCCAATTAAAATCAATTATCCATTCTTTGAATTTGTATGGCGAAAAAATGCATTTTGTCTTAATTCCAAGAAAAGATTTTTTGAATTTAATATATGAAGATAAATTGACTATAACTAAAAAATTATTTGAAAAGATTAAAAATGGAAAACAAAAATAGATCAGACAAATTCAACTATTTTTCAGATTCTAATTCCGAAAAAGAAAAGAAAAAGAATCTCTGAAAGAAAATATGATACTGAACCAAAATAGTTTTATACACCTTGTTATTTGGTTTAACTATGACAGGATGTATTCAGACTATGGTTATCAAGACCTCAACATATACAGGTGCAGGTACTGAATATTACACATCATACGATAAAATTAGTCCTTATGTAACATCACTTGAACCAGCTAAAGCTGATAAAAAAATCGACAAAGATGACAAGGCTTTACAACCTGGTGAATTCAAGAGAATTGTTAAAAATGATTCTACAAACTACCATGCTAGCTACAAAAATCATGCCGAAATACTTAAAAAGTTAAGAGAGCAAACAGGCGGAGATGTTTATGGAAAATATAACTCATATTCATCTTCTATTCAATGATTCTATGACACTCATAATGCTGATGGTAAATGAAAAGCAAACTATTACGATTCTGCAATAGTTAAAGGTGAAAATGGTAAATATCTTTTTGATATGAGCGAATCTGCAACATATAAACCTACCTACACAACACCTTATAGTGAAATTAAATTTATTGACCCACAATTCAATTTAAATTCAATTTTATCTTTAGTTGATCAAAAAAATCCAGATCTTTATGAAGTTAAAAAAGATCGTTTAGAAAATAAAACATTCAAAACCCCTAAAAAAGCAACATTGTTTTCAATAGCTACACCAGACCAATTGAAAGCAAAAAATAAAATTGCTGGTAAGGAACTTAGTGAAATTGAGCTTTCAAAACAATACTATGCTCGTGATGTTTTAGAATTTTTATATAGAAAAACATTTATTGAACAAGTAAATGGTAAAGATAATTATTTTGCTAGAAGATTCAAACAAGAGTTTGGTACCGATAAATACAATGATGTATTAGGCAAAATAATTGATCCTACAAATACTAACAAATATAGTAAAAAACAAGTGGAACTTATTTATGAATATGTAAATATATTAAATGGATATCTTAAAACTACAGGTTTCACAAATATTTATGACGACACTCAATTAACAAAAGTTGAATTAGCTAAAGGAACTGAAAAAGTTAAAATAGCTAAAGAAAATGCTTATCTTTCTCCTGCTCCAAAACTTGTATACACTCATAGTGAACCATTCAAACCTTTTGTTAAATGAGGTGATTCATGAAAACGCTGAGGTCCATTCTTTGGATTATTCGTTCACCCTATTGCTTGATTAACAGGATCAATGCGTGAAAATATGCCTTCACTTGCTGGTTGAAGTACAATATTTGTTATTATTATTGCTGTTGTTATAACTCGTCTGCTTGCTTTAGCAATTACTTGAAAATCTCAAGTTAATCAATCTAAACAAGAAGATATTAAAGCTAAAAAAGCAAAAATTGATGCTAAATATGCTGACTTCAAAGGCAATAAACAAATGAAAATAAGACATCAACAAGAAATTGCGGCTTTATATAAAAAGAATAATATAAATCCTCTTGATGCTTTCTTGAATATAATTATTTCCTTCCCTATCTTCATTGCTATGTGAAGGGTTATTCAAAGTATTCCAGAAATGAAATCAACTCATTGACTAGGTATGGACTTTGCTGCAACTAGCTGAAAACGTTTATTTGCTGGTGAATGAGCTTACCTTGGAATATTAGTTGTTGCTTTAGCAGTTCAATTCTTCTCGCAATTTACTCCACGGTTACTGAACAGGAAAAAATTCAAAGAAAGAACAACTATCGAAGAAGAAAAAGCATTGAAGAAAAGTAATAAGACACAAAACATTATTATGATTGTATTCTTATTCATTACTTTAATGTTTAGTTGTGGTGTCCAAGTGTATTGAATCTTCAGTGGGCTTTGAAATATCATCCAAACAATTTCAATTCACTTCTTCAAAAAATCTGCATATTATAGAAGAAAATATGCAAATGCAACCGTTTAAAAATAATCATAAAACCAGATCATTCTGGTTTTATTTTTACTCTTTTTTAAAATAATTTATTAAATATTTTAAGAGTCGACTTTTTAAAATATAATAAAGAAATAAACATAAAAAAAATAGGAGAATAATATGAAAAAAATACATGCTTATGTAGCTGGTCCATTGTTTACTAGAGCAGAAATTGACTTAAGATACGCAATCGAAGAAACAATGAAAAAAGCACTAAAATCAAAAGAATTAAAAGGCAAAATTGACTTTGACATTTTCAATCCGATTCATCTAAATGAAGAATTAGAACAAAATGGAAAATTAACACCACAAGAAATTTTCAAAAATGACTTAGCAGCAATTCAAAAAAGTAAACTTACAATTCTTGACATTGACAACAAAGATGATGGCACCATGGCAGAATTTGGTTACTTCTTAGCAATGAAAGAAAGAGATCCAGAAGTTAAAATATGTGTTTGAATGTCAGATTTTAGAGATGTAGCAGATCGCGACATTAGATTAAACAGATTTATCAACGGAATGATTTATGTATCAGATGGTTGCGTAAAAAATCAACAAGAACTATATGATTGATTAATTAAGGCATATAAATAGAAGGGCTTAGGCTCTTTTTTCTTAATAAAAAAGGCTTTATTTAATTTATAATAGATATATAAAATTTTAGGAGACTTATGAAAAATAATTTAATCGAAAATAAATTAATTGCTTCAATGCGCGGCATAGCATTGGATTCAATCAACAAAGCAAAAGGTGGCCACATTGGTATGGCTATTGGAGCTGCAAATATTACTTATTCATTATTAGGAAAGGAATTAAGATTCTCAAAAGAAGATCCGAAATGAATCAATCGCGATCGCTTTGTTTTATCAGCTGGACATGGTTCAATGTCATATTATTCAATTATGCATTTCTTAGGTTTATTAGCAAAAGAAGACATGCAAAATCATAAAAAAATGGACTCAAAAACACCTTCTCATCCTGAGTTAGAATATACACCTTTTGTGGATGCTTCAACAGGTCCTTTAGGTCAAGGAATAGCTATGGCTACTGGTATGGCTCTTAGTCTAAAAGTACTACAAAACAGGTTTAATCGTAAAAATTACAATATTATAGACCACTACATTTATGCGCTTCATGGAGACGGTTGTATTCAAGAAGGTGTGGCTCATGAAGCTATTCAATTTGCAGGTACAAATCAATTAGATAGACTTATTTTAATTCATGATTTTAATAATGCTCAAATTGATACAAAAACCGAGGTTGTTAATAATATTAACTTCATTAAATATTTTGAAGCTTGTCATTTTGCAACCTTTGTAGTTAAAGAAGATAAACCTGAATTAATTCTTGAAGCAATTCAAAAAGCTAAAGCTTCTAAGAAACCTTCATACATTCAAGTTCACACAGCAATAGCTAAAAATACACCTTTTGAAAACTCGCCAAAAGGTCACCATGGAATGTTAAAAGAAGAAGATACTATTGAATTTAAAAAGAAATGTGGTTTAACTTCATTTGTTCCTTTTGAATATGACAAAGAAGTTTATGACTATGGAGAAGAATTATTAAAAGAAAAAGACAAACATTATAAAGAATGATTACATCTTTATGATAAATATAAAAAAGAATATTCTCAAGAAGCTAAAGAATTAGAAGATTTATATAATAAAAAAGAAAAATACAATTTTGATGATATTGAATTTAATGAAAGTAATGCAGCAACTAGAAACTATTTTGCTCCTATAATGCAAAAAATAGATCAAAATTACAAAAACGTTTTAGGTGGTTCAGCTGACTTAGCCTCAGCTACAAAAGTTAACTTTAGCAAATCTTATGCTGAAGGTGGCAAAAACATTCACTATGGAATTAGAGAATTTGCTATGACTGCTATAAATAATGGAATCTATTTACACTCACATTTAAGAACAATAGATTCAACATTCTTAGCTTTTAGCGATTATGCTAAAGGTGCTCTAAGACTAGGTTCAATTATGAAAATACCTTCAGTTCATGTTTACACTCATGATAGTTACCAAGTTGGGGGTGACGGACCAACTCATCAACCAGTAGAACAATTAGCAATGCTTAGAAGTACTCCAAATTTTAAAGTTATTAGACCTATGGATGAGAGCGAAGTCAAAGCAGCATTCAATTATGCATTAAATCAAAATGAAAATCAAGTAGCTATTATTGGTTGTCGTCAAAATGTTAAATCTTACAATTTACATCCTGAAGGTGAATTGCAACCAGCTTACATTATTCGTCCTGCAAAAAACCATGACATTAGTATTTTGGCTTCTGGTAGTGAAGTTGACTTAGCAGAAAAGGTTGCTAATAAATTAGTTGGAGCAGGCATTAAAGCTCAAGTTATTTCCGTTCCTTTATTACAAGATTTAGTTAATGATGAAAAATTAATCAAAAGCTTAGGCATTGACAAAAAACCAATATATGCTATTGAAGCTTCAACAGATCCATTGTGATATAAACTTGGAAAATATAACAAAATTGATGCCTTTTTAGCTGATGGCTATGGTTATTCAGCTGATGGTTTTGAAGTGTACAAAATCAAAGGATTTAGCTTAGATAATATTCACAAAAAAGTTTTAGAGTTCTTAAAATAAAAAGAATAAACACAAAAAAAATATTTAAGAGATTTCTTTATTTATTTAACATGGATACATATAATTTTTTTCACTAATAAATTTTAGATACGGCTTATTTATAGGTAAATTTTTCAAAACTTAGTTGTTTTGATTTTGTATTGATATATAATAAATTAACACAAAAAAAATATAAAAATAAAGGGAGAAAAAATGGCATCTACAAAATCAAGAACAGTTTTAACTATTCTTTCATTCTTCCTTGGATGATGTGGAGTTGATAGATTCTACTCTGGTGAAGTGCTTCTTGGATTTTTAAAATTGGTAACAGTTGGTGGATTAGGAGTATGATATCTTGTCGACTTTATTCTAGCTATTACTGGCGCACAAATAGATGGTCAAGGATACATTATTAGCGATTGAAGTCTTTCATCTACACCTGTAAACGGAGGACCAAGAATCGAAACTGATGAAGGTCCAAAAGACAAACACAGCTCATAATTTCAAGCTATTAATGAACTTTATTCTATTAATTGGAATAGAGTTCATTTTTTATTCGAAAGTTATTAATATTATATTTAATGCTTTCTTTAATTTTTAATTTTTTTGAATAAATTGAATTATATTCAAACATATATAGTGTTATGTAAAAAAAATAAATGATTTTTCTGTCATTTTTAACCATTTTTTGCAAAAATTGTAATAAAATTATAAAGTAACATAATAGGAGGAATAAATGTCAGCAAAATTTTATCAAGTTATAGAAAATCAAGGGATTGGCACACAAAATTGATTAATTTGAAAACATCCAGAGTGTGAAGTAAATAACAATTCAAAATTAATCGTTAGACCAGGTCAAGTTGCCATAATTGTTCAAAGCGGACAAATGATTAAGCTTGTTGAACCAGGAACAAGTAATATTGATAGCGATTGATTCCCTATATTAAAAAATAAGACAAAAGAATTTTGAAATAAAAACCCATATCCAATTGAATTTTATTTCGTTAACACAAGATTAAAATTAGACTTTCTTTGAGGAACAAATTCGCCTATTCCACTTAGAGACCCTAAATATGGATTGCTTTTATATTTAAGAGCAAGAGGTCAATTCGGTTTAAGAATTACTAAATATGAATACTTATATAGAACATTAAATGGATCATTTGATTCAAGTAGAACAATTTATTACGAATTCATAGACAGAATCTTAAGAGGCTGAATTAATCAAAACATTAAAAAAATTATTTCAAAATTCATTCTTGAACACAAAATACCTTTTGATGAAATAATCGCATCAATAGATAATATTAATGAAGATTTCAAAGAAGCTTTAAAATCTGAAACACAAAAATTAGGTATGGAAGTTGTTAACTCAAGCATTGAAGATATTAGTATACCTAAAGAGCAACAAGATGAATTGAACAAAATTCTTAAAAGAAAAGCCGAAGTTGATATCATGGGAGACGACTATGCTAAAGTTAGAGGCTATGACGTTTACCAAGATGCAGCAAACAATTCGGGTTCAGTAGGAACTTTTTTTGGAGCAGGCATTGGTTTAGGACTAAACGAAGGTGCTTCAAATTTATTAAGTGATAGCAAAATCAATAAATCAGCAACCCCAACAGCAGCAGCTGCATTATTAATCACTTGCGGTACATGTGGAAGCAAAGTATCTAAAGATTCTAAATTTTGTTTTGAATGTGGTTCAAAAATCGCAACAAACAAATGTTCATCATGTAATATCGACTTATCGCCAAATGCAAAATTCTGCGTAAATTGCGGAAAGAAGGTGAAATAAAATGAATAACAACAATCAACAACAAAAATTAGTTGAACCTAAAACAACAATCTTTAATGATCGTGAAACTATTAAAAAAAGATGACCATGAATGCTCGCTTTTGGATTAATATTCATCACAATGATTTTAATTCCAATTGGTGTTAAGAAATTTCCATTTCATGACAAAAGCACCGACATGTTGTATTGATATATTGGTGTTAAATGCTTCGCTTTATTGTTTTTAATTTTCGGTTTTGTACACACAACATTCTTCAAAATAGCTAGATCATTCTACTATAGATTAATGGTAATAGTGGCCCTTTATCAAGTTGTGCCATTTATATTAAGATTTTTTGGTTACTTACCTAATTTCAACAATATCAACATTTGATTATCACTTTACTTTGTTGTATCACTTATTATCTTTATCGTTCCATATGCTACTTTAACATTTATAAGCAACAAAATGAGTAAAACAGATCAAAAATTTAAATCAAGAGAAAGATCACTTAGAGAAGAATACATCACAGGACAAAAACCTAAAGATGAAGAAGGGTTGTACTAATTATGGAATGCAAATCATGTGGAGCTAATGTTAGAATCACAGATAAATTTTGTTCTTTTTGCCGATCATTAATCGAAGCACCTAAATCAAGCGTTGAAACATCTCAACACACAACTGCAACTACAAGAGTAGTAACAGAATCAAAAGCAATTAATTATGATGTATTGAACTTATTAGTTATAGAAGATAAATATGACGAAATTGATTTTGATTTATCTTCTAACACAGCTGCAAACTCAATGAAAACATATGTAAACAAAGGAGGAAGCATTTCAGAACTTGAGAAAAGTTATGTTTTAGCAAATAAAATCGATTACTATGAATCACTTGACAAAGATGAAATCTTAAAAACTTACTCAGAAAAAATTAAGAAATATTCCGGTAAAGATATTTGTTGTTTAAAAAGAAGTGAATTACAGGCCTTAGAAAGAAAAATGAGTTTATATGAATTCAATGATTATCTTAAAGTATTAAAAGATAGAGTATTTAATAAAGAAGGAATTATGCTAAGTGACAGCGACCTACTCGACAAAATAAACTCAGATAGAAACATTGAAGTTTTTGCTATTTGTTCTTATGACTTCTGAGACAAAAAAACTGTTGAATTAGATAGACAGAACAAAGTCGAATATGAACAAAGAATAAAAAAAATTAATAACAAAAAGAAAACAGGTAAAATCATTATATGAACTTTTGTTGGTATTTTTATTGCTACATTTATAATATGTTTTTGAGTTATTTTTAGTGTATCAATTAGATCTTCTGTATCAATTGCTTCAACATCAAGCAGTTATTACTAAAAACAAATTGACATCTTTTTAAAAGGTGTCTTTTTTTATTAACTCCAAAGCAAAAAATAAAACCTAAACTAGTACAAAAAGACTTAAATCTTTTTGGGATTTAAGTCTAATTTTTTTGTCCTGGTTTATTATTAAACTCAATTTAGTTTACTATCATGTAATAGTTGATCTAAATAAAGCAGGGAAGAATTTAGACATAATTTTTTTACCATAAATTACTTCCATAATTCATTGTCTTAATGCATCATGTTTTTCTACTTCATTTTCAAAAGACAAGTGTTCAAGTTTGATTTTTTTATCAATAAATCTTGATACTAAATTGATTTCATTCATTTTAATTGCTTCTAATTCTAAGTTTATTTTTGAAATATAAGCTATCTTTTTTATGTCTGTTTCATAGTCATTATTATTAACTTTATTTCATCTTTCAATAGCATGGTCTGAAACCATAATATCAAAAGTTTCTATTGCTTTATCTCTTATTTCTCTAAAGAATTTTGCATAAACATCATGCTTATATCATCTTTTAATTTCATTTAATTCTTCTTTTGCTAAATTTTTTGAACCGCCTGGATTATATTCCATTGCATATTCTTCTAATTTATTAATTGGTAAAGGATCAAAAGCTGGAATATAGTCTTTAGGAACTAAATTAATTTTAGCAATTTCATTTGGTTTGAATGTTTCCATGATAACTTGGTAGTCACAATTATCTAAGGTTAAAGTTTCTTCTTTAACAATTACATCAAAGTAAATAAATGCTCCATATTTCTTTTCTAATTCAATTTTTTGTTGAACAATTTCATTAAATTTTTCATTTAAAACTGTTTTGCCACTTAGAGCAATTAGCATAGGTTTTGAAATTGGTTTTGAGTGTTTAATTTGTTCATTCGTTGTTAATTTAATTCAATCAAATTGAGGATAATTTTTATCCAAAGTTGTAATACTTGATTTTACTAAAGCTTTCATATTAACTCCTTTTATAATTAACTCAATTTCTTATTAAATATAGCATAAAACAATGGTTTTGGTAAAAAATTAATTTGTTTAATTCAAATATTTTTTAACATTTTTATATGACTTTCTATGAAATTCACAGATGCCATATTGACTCATTGCTGCTTCATGTTCTTTTGTACAATAGCCCTTATTTTTCTTGAAATTATACATAGGAAATTTTTTGTCAATTCCAACCATAATTAAGTCCCTAGTTACTTTAGCTAAAATTGAAGCTGCTGCCACATTAATACTTAATTGGTCACCTTTTACTAAATTAATTTGAGGTATATTGATGTCATTAATTTTTTCAAAATCTGTAATTACTAATTCAGGTTTTGTATTAAGTTTATTAATACACCTTTGCATGCCAATTCTTGAAGCTTGCTTTGGATTTAGTTGATCTACTTCTTGAGCATTAATAATAGTTGTTACTGTTTCAATAGCATTTTCAAGAATAATTTCTAATGCTTCCTCACGTTGTTTTTGACTCAATTGTTTAGAATCCTTTATTAAAGGATGGTTAAAATTTTGAGGCATAATAACACAACCAACTACTAAAGGCCCTGCACAACAACCTCTTCCTGCTTCATCTAAGCCTACAATTAATTTAGTGTCAGGATAATTTTCTTTTTCATAATTCAACATAATCAAAATTATACAAAAAAAGCAAAACTTGAATTCTGCTTTTTGTATTGAAATTATTTTTTGTCTTTGTTTGCTTCTTCGTCTTCTTTAGCCTTTTGAGTATCAACAATTTGAACAATTAAGCGGCATAATTCTTCTTTGTCTATTTTTTTGTAACCACTAATGTATAGGGTTTCTGCTATGTTGTAAAGTTTTGCATTAGGCAATGCTAATAATTTTTTGATTTTTTCTTCATTTTCTTTTTCATTATTTTTAAGATGTGCTTTGTTATCAACTATTTCAGCTTCTTCAATATCTTCTTCTTTATTGAATTTGCTTTTATCTTGATTAGCACTTACATCATCAACATCATCTAACATGATTCCAAAAAGATTGTTTGCTAAATTGCTTTCATCCATCATACCTGATTCTTTCATCTTTTTTTGAAGTTGTTTGATTTCTTCTAATCTTCTTGCATTAATAAATACACCTTTGAAGTATCTTAATTGACTAACAAACAAGAATTGGAAAACAATTAAAACAACTGTGATAACTGAAGTAACTATTGTTATTGATAAAATAATTGGATTGTAAGCAGTAGCTAGTGATCTAATGTTTCTAATAATTGAGAACAAGTTTATAAATCAAGAAATGATTAAAACCATTGAAGGTCATGTATGCATGTTGGCTAATGATTTTTCTTTAAATGTTCGAGCATAAAAAACTAAAAATACAATAATGCTAACTAACATACCAACTGTAACTAAACCATTTGTAATAATCATGCTCATTATTTGATCTTCAGGTTTAAAACTTGGAGTTGTACCACCATTAATTTTTACAATATAGTCAACTAAATTATCTTTAAAAGCAGCGAGCAAAATTGTTGAAGTTAAAGCACAAGCAAGTGCTAAAAAGAATGTTAAAAGAGAAAGAATTACAACTGATCTAAATCTTTCTTTTTCTAGAGCTCAAAGTTTATTTGTACTTTTAATTTGTAAATATTTTTTGTCGAATAAATTCATTTTTTCTCCTATATCTAATTAATATATTATAAAACATTATTTTTTAATAGTTCATTAAAATCATAATCGGCTACATCAAATTCTTTAGGCACCGGTGCATAAACACAAATTTCTTTTTTAGTAAGTGGATGAATAAATTTTAATTTATAAGCATGTAATCTTTGATTAAATTCATCAACACTTTTGCCATAAGTAGGATCACCGTAAACTGGATGTTTGATGTAAGACAAGTGGACTCTAATTTGATGAGTTCGACCTGTTTTTAATTCGCATCTAACTAAAGACATTGGTTCTTTATCTAAATAAAAGTGCTTAAGCAAATGAACAAAGGTTATAGCTTCTTTACCTTCTTTATGAACTGACATCATTTTACGATCAACAGTATTTCTAGCAATTGGTAAATTAATTTTAGTAAGTGGATTTTCAATTAAGCCTCTAACGATAGCTAAATAACTTCTTTCAATTTCATGTCTTTTGAGCATGTCAGTTAATGAGTTATGCATCTTGTTATTTTTAGCAATTATTAATAAGCCACTTGTGTCTTTATCAATACGGTGTACTATACCTGGTCGAAGTAACCCATTTTCATTTGATAAATTGTTTTTAAAATGATATAAAAGTCCATTAACTAGTGTGTCATTGTAATGCCCCGGTGAAGGGTGGACAACTAGATTACTTGGTTTATTAATTATTACTAAATCATCATCTTCATATTTAATGTCAAGAGGAATATTAACTGGTTCAATTTTAATTTCTTTGTCAAGTAATTTAACAATTGTAATTTCTTGTCCTTCGCGAACGATGTAATTACATTTATGTACTTGAACGTTGTCATTAATATAAACTGCACCTTGCTCAATTAACTCTTTAATATCATTTCGACTTAATTCACTATTTTGACCGATATACTTATCGATTCTCTCTTTATATTGAACACTAATTTTTAACATAGAGTAATTATATATTATAATTAAAAATTCAAAATTGTTTTTAACCTAGTCAAGGTTCAAAAATAATTAAAGCTAAAACTATGTTGCTTAAGATCAAAGCGACCACAGGGACTTTTTTAAGATAAAGAGTAGTTGAAAGTATCACTCCAACAGGCACATAACAGTAAACAGCAATTCGACGTCAACCCCTATAAAACAGTGCTTTTTGTGTATCATTATTTATTCCTACATAGTTAGTAAATGATTTATTAAAGAAAACTTGAGGTGCTACTGAAGCTATAAAAAGTTGAAAACCCAAGGCAACAATAATACCTGTTACAACAGGATTCATTATGTTAATTAATTTTATTAAATATTTATTATTTTCAAATTTTTTTGAATATTTCATTGCTAATTTCATCATTAAAATTGGTGGAATAACAAAGACTAAATAAGTTAAAAACATAACTATAAATCCTCATCATTCTTTATTAGCATAGAAGTATCCCGTAACTAAAGCAAACTTAGTAGATAAAATTCCAGGTGTTGCATTACCAACAGCAAATACTTGGCTAATATCACTTTCAGTAATATTACTTCCAAATCATTCTCTAAATAATCCTCAGAATCAACTAAAAATAGGCATAAATATTTGCCCACCGCCAAAAACAGATAAGCTAATTAATACCAAAAAAGGTAGGGCAACAAGTAAAGCAAGTCAAGCCATTATTGACCTCCTTTCTTGTCACTATTCAAATCATTTTCTTTGGTCTCTAATTCTAAGTTTTGTTCTATTTTTTCTTCTATGTTATTGTCTAAATTAGTAACTTTATTTTCAATATTTTCTTTAAGTTTTTTGATTTCTTTTTTGTGTTTGATTGCTTCAACAGTTGCAAAAATACAAATAATTAGAACCATAATTATTACAGGCATATTTCATGGAGTTGGTATAAAGAAACTAAAGGCAAAGGTTATTAAAAATAAAATTAATCACAAACTAATTTTAGTTTTGCCTCTTGATTTTTTGAAGTATTCCCAAGCAAAACCAATCAATGATCCAATAATTGCAACAATAACACCAAGTTCAATAACAAACAAATATTTTTGAGGAATATGTTGCACAAAATAAAAGATTAAAAAGAAAACTAAAACATGAGGTAAGGTTGCAAAAGTTGCCAAAAACAAGCCCTTTTTGAAACCTAAAAGTTTAATAGTAATATAACTAATTGCTTCAATGACGCTAGGACCTGGAAGCATATTTGTAATAATTACATTTTCATCAAATTCTTTTTCATTTAATCATTTATATTTATCAACAGCATACTTTTTAATAACAGGCATCAAGGCATTGCCACCCCCAAAACCTATGAAAGTAATAAATAAAATAAACAAAAAAACATTTCAAAATGTTGGTTTTTTTGTATATTGAGTTGCTTGTTGATTTTGATTATTCATATTTTGAATTATACTTTTATTTTTAAATTTTTTTAGTGCTTTTTTGTGTACAAAAAATTTAATTTAAATAAGATCAAAATTTAAAATAACTTACAAATTTAATATAATTTTTATAACAAAATTATTTATTTATAATATTTTTAGACAAACAGGAGATATTATGAATGCAAAAAATGTTTATCAATTGCAAAGAGAAAAATTATTAAAAAATGCTTTTATTGACAAAAAATTGAGAAAAGAAAATATCGAAAAACTTTACTCAATAATTGTGCAAAATGAAAGTAAGCTTAAAGAAGCTTTATCTTTGGACCTTAATAAATCATCACTAGAATCATATTTTAGTGAGATAGGTTTAGTCTTAAAAGAAATCAAATTTAATATTAAAAATTTAAATAAATGAACTAAGCCTTTTCATGTTTCAACGCCTTCTCATTTAGCTTTAGGAAAAAGTGAAATTCAATATGAACCTTTAGGTCAAGTATTAATAATTAGTCCTTGAAATTATCCTTTTTATTTGAGTTTAATGCCATTAGTTTCAGCTCTTGCAGCAGGCAATAGAGTAATCATTAAACCAAGTGAATTTTCAGTCCACACTTCACAATTATTACAAGAATTAATCAACAATAATTTTGATCCTCAATATGCTTTTGTAGCAGACAGTTCTTTAGAAGTTGCTCAAGAATTGTTGGACTACAAATTTGATTTAATTCATTTCACAGGTAGCACAACAGTTGGAAAAATTATTGCTAAAAAAGCATCTGAAACTTTGACACCAATTACTTTAGAATTAGGTGGGAAGTGCCCTTGCGTTGTTGACAAAAGTCAAGACATAATTTATTCTGCTCAAAAAATTATGGCTGGTAAATTGCTTAATGCAGGTTAAACTTGTATAGCTCCTGATTATGTTTTAATTGATAAAAATAGAATTAACGCATTTAAAGCTGCTTGTTATGTTTATCTTGAAAACGTTTTGCAAAATGAAGAATATATTAAAGGTAATTATCCAAAGATAATTAATCAAAAACATTTTGAAAGATTAAAAAAATATTTACCTAAAGATCATAAAATTAATGAAAAAACATTACAAATAATGCCTTATATTTTTGAAACAAGCTGAAATGATCTTATAATGCAAGAAGAAATTTTTGGGCCGTTGTTGCCTATTATTCCTTATGATAATTTAGATGATTTATTAGTCAAATTTAAATCACTTCCTAAACCTTTAGCTGCTTATTTATTTACTAAAGATAAGAAAATAATAAAGCTATTTAAAGAACAAGTTACTAGTGGAGCTTTAGTAATTAATGATACCATTATGCATATAGCAAATAGTAATTTACCTTTTGGTGGTGTTGGTGATTCTGGCTATGGCAGAAGTCATGGTTTTGAAGGTTTTAAAGCTATGTCAAATCCAAAAGCTATTTACCAAAGAAAAGGTTTTAATTTTAAATTAAATGAGCATCCTTACAGCAAGAAAAAAGAATTCTGAATCAAAAAAATAATTAAGTAGATTTTACTTGGAATACTATAAAACAGCATAATAAATCACACTTAGTGTAAAAAATCAGTGTGATGTTTTTTAAAATTTTAGTTAGTAATCTATTTAAAGTTCTTTTAAATATTGTTTAAATATTAATAAAGATTCTTTGAAACTTTGTAGATAATCAAAAAAGAAAATAATGCCAAAAATCAAACTAATAGTTATGCAAATAACGTTAATTATTTCAAAGCTTATTAAGAAATAAATAAGGAAATTTGCTTTAGTTGAAACTGCTACTTGCGAAATAGTTAAGATAATTAAGATTACTCCTAACGATATATTGACATGCGAAAAATATTTTAATTTTTCATGACTGTAGCCAACAATAATATAAGGCAACATTACTAATATTGTTTGACCAATTAAAGATATAAAAACTCCACTACCTTTTTGTCAACAACAAACTAATACAACATTAATTATTAATAAAACTAAGCCAATTATTACATAGCTTAATAGCATTCATCATCTCTTTTGTTTATTCATTTTTACTCCTTAATAATTATATTTAAAATTTTTATTTTTATAGCCTTGTATGTTCAAGTTATAAAAAAATTACGTAAAAATTAAATTATAATTTTAGAAGGAGATAAACAATTATGAAAATAGCAACAGCAGATATTGGCGGAACAAATACAAGATTCGCTTTATTTAAAAAAAATAAATTTTGATCTAAAGTTAAATTTGAAACTAATAAAAATAATTATCGTGAAACTTTAAATCATATTGCTCATTTATGCAATTTGCATAAAATAGAATGCTTGGCTTTATGTATTCCAGGACCAGCCGACTATGACAAAGGAATAGTTTTAAATACACCTAATTTACCTGGTTGAGTTAACAAAAACATTAAAAAATATTTATTTAAGCATACTAATCTAAAAGAAATAATTACTGAAAACGATGCTAATGTTATGGCTTTGGCAAATCACAAATATTACAAACAAAAGAAAAGTGATGTAACTCAATTTTTCACAGTATCAACAGGTTTAGGTGCTGGTCTTATTATTAATGATCAAATATATGTTGGTAAAAATCACTTAGCTCAAGAAATCGCGAGAGCACCTTTAGGTACTGATGAAGATGACTCATTTCATTTACTTCCTTATTCAGTGGAATTGTATGCTTCAGGAACAGGCATTGAATTAAGATATAACAAATTAACAGGAGAAAAATTAAACGCTAAAAAAATTTTTGCTTTATATCCAAATAATGAAAATGCAAAAAAAATAATAAATGAAGGTATAGATTCTTTAGCTAGAACTATCTCAACTTCTTTAGCTTTTTTAAATCCAAGTCTTGTTGTTTTTGGCGGTAGTGTTAGTGAACATAATAATTGGTTTATAGAAAAAGCTATTGAAAAAGCTAAAACATATACAATTAAATCTCAATATCAAAATGTAAAATTTGCTTTTGATCCTTATACAGATTTCAGCGCTGTAATGGGTTTATATTGTTTAGCAAAAGATAAATTTAAATAACATTTTTTTCTAAAAATACTTTAAAATAGAATTATTGGTAAATCAATAATTTTTTTAAATCATAATTAATGAAATTTTTACTATTAATTTGTATAATTTTAAAGTAATCATTAAATTAAAAATGATGTAGAGAAGAGATGAATTAAATGAAAAAATTCAAGTGACTTTTAGCTCCAACAGTTATTGGTGTTTTATCTGCGCCAATGATTTCAGTAAGCTGTTTAGGAAATCAAGAAAAATGAATAGCTTTACAAGACTTTGTTGACAATTTAAAAGAAGGAAGAGATTATAATTTTTCAATTTCTACTGACGACTTAAAAAAAGAATGCAAAGCTATAGCTGATGAATGAATAAATAATCCAGAATTCAAGAAGAAATATGCAAAAGATGTATTGCCTCCTTCTGCAAGACAATTAATTGATAATCCTTCTGCAACAATAAATCCTGAACCATCAGAATTTACTGCTGAATATTTATGAATAAGAGGATATATAAAAATTGAATCTAAAAATCCTAAATATAGATTCGATGTTCTAGAAGTTTATGACAGTGGAACATTAAGAAATGCTCGAGCTAGGGTTGGTTTTAAAATTGTCGATGTTTCTGATGAAAATTATTATCAAAAGATTAATACTAATAGCTCGCCTATTAATATAACAAATACAAGAAGTTATGGTAGTGAGCACTTCCATGTTGATTTAACTAAAATAAGAGAAAATGAAAGAACATTATTGAAATTATATGCATTTAATCTAAAATTAAATGGTTTTTATGAGTATTAACAAACACTAGTAGAAAGAAAAAAATGAAAAAATATTTTTTGAATGGTTCTATTTTAGGTAGTACTTTATCTTTTGTTCCAATAATATCTGCTTCTTGTGTTCAAAAACAAGAAGTTCCTTTTGAAGACAAATTAATTGAATCAAAATATGAAAATAGTTCAATAATTGGCTTAAAGGACAAAGATTTACTTAAATTAAATTTAAATCAAATAAAGTATTTTATCGAGCAAACTAATAAATTAAGATTAAAGAATAATTTTAAACTAAAACAAGTTGCAAAAGAAAATGATGATCTATCTTTATTAATAAATGATAAAAAATATTCATTAAAAAGTTTTTTAAAATCAGTTAATGATAATTGGGATAAATTATCAAAAAATTGAGAAATTGTTACAAATAACAATATTAGCTTAATTAGAAAAGGTAACAATAGTACTGATGTAAATGTGTTTTTTGAAAATGATAATTCAGATTTTAAAAATTTAGGCTATGATAAATATGTTAAAAGATTGTTTAAATCAAAATACAATAATTTAGCAAATGCTTCTAAAAATTTACCTGATTTGCAAATGATTTTACAAGTTGCAATTTTAGGAAGTAATAGATTTTCAATTTGAAAAAATCAGCTAAGTTCAAACTTATTAAGAATTAAAAATATTTTTGATGAAAATGCTATTTTGAATCAATTTAAAGAATACTTCGAAAAACAAGCAACTTTTTACTTAAAAGCCTATAAAATAGTTAATAAAGAAAATAAAGAATTTGAAAAATTAATTATTACAAGCAAGGAAAAAGAAGAGTACAAAGGCAAGTATATTTTAAAAATAAAAGTAGATTTATTGGATAAAAATAATCAAAGTATATTAAATAATGATCAAAAAAATAAAGTCTTTTATTTGACGGATTTTCAAGATTCTAATCCTAGATATCATGCATTTCAAAAATATGGAAATTTTGAAATTGATTCTTCATTAGAAACAAATGAAAAGAACAAATCTTTATTTAATGAAATGTTTAAAGAACCGACACTTTCATTTGAAAAAAATATGTTCAACTTTAATGATTTTGATTCATTGATGCACCCCTCAAAACCTTATTTAGGTTTTAATCTTAATTCTTTTATGCAATTCTATAACGCTTATAAAGAAAAGGTCAAAATTAATGTTCCTGATGAAAATTATGAAGCAAAAATAAAAGAAATAAAATTTGACAAAATATTAAATAATTCACATTCAATAGGCAAGGCTATAGTTGAAATTAAAAACAAAAAAACTAATAGAGTACAAGAAGCTAATTGGTTTACTATTGACTTTACATATGCACATAAACATATATTTGATGGCTTTTATATTCAAAATAAATTAGATTGTGATCCTTTAAAAAATGATGAATTCTTCAGCTATTATATAAATAAAGGAATTGGAACAAATAATGAATTAAAATTACCTGAAGGCATTAATGCAGAAGAATTTTATAACACTAATTTAATAGATATTGTTAACTTTATTATTTATCAAAACAGAGATTCATTTAACATTTGAAATAATAAAAAAATGAACAAAAATAGTGTTCTAGAAATAATTCAAAATAAAGCAGAATTTGAAAAAAAAATAAGTACATTAATTTCCCAATATGTCCTTAAATTCTTTATAAACAACAAAAAACCAGGTTATGAAGAATTAATAAAAGAAGTTAAAGTTACTATTGATCCAAATAATGATTATGACGCCTTAAAATATGGCGTCGGTAAGTTACCAATTAGAATAGATTTCATTAATCATAAAAATGAAGATATGATTAAAAACATAAGTGAAAGAAAATTCTTATTAGGCGGTTTTGTTGGTTATGATTTCAGTGATATTGAAGCAAAATTAAAAACATTAACTGATGCTGATTTTGAGAGACCTCTTAAGGATAAAACTCCACCTTATTTAATCAAAAACTAAACAAAAAAACTTGCGATTGGCAAGTTTTTTTCATCTGGCCGGTAGTAAGGGATTTGAACCCTTGAATGACTTTCACCATTATTGGTTTTCGAGACCAACCTCTTCAGCCTCTCGAGCAACTACCGTTAAAAAAATTATAAATAAAAACTCAAATTAATGAGTAAATTATTTATGATCTTTATGATCATGAGATGCGTGTTCGTTTGTGTGTTTTAATTCTTTTTCTTTTTGAATTGTTTCATCAAAACGATGTAATAAATCGTGTTTCATTTCTTTTTCTTCTTCTTTAATTCTTTCTTTAACTCTTCTTTCAATTTCTGCATCAATTATTTCAGCAAGCTCATTAGCTTTTTCTTTATTATCTTTTTTCTTTTGAAAACTAGTTAAAACACTTGCTGCTACCATACCACCAGGTATAGCAATAATTGCAATACCAACCATAGAAATTAAGGGCACAATCATTTTTGTAAAACCACTATAAGGAATATAGTCTCCATAACCAATAGTTGTTAAGGTTATGGCACTAAAGTATAAGGCATCTCCAAAGTTTTTTACAATGTTGGTGTGTTCTGTATAAAACTTAATTTTTTCACTTTCAAATTTAGTTGGATTAGCTTTAACTTCAGGTAATTCTCTAACTAAATTATTTACATATTCTGTTTCTGTATATCAAACTGTAAGAGCAAATAAAACGATCAAAATACCTATAAATAAGAAGATATTGAAAAACAAAACTTTTTCATTTGATACAGATTCTGAAATATTTTTAAAAACTACAAAAACATTTAAAAGCATAATTAATCTTAAAATTCTTAAAAACTTAAAGCCGTTAAAAGCTTTTAATGCATTACTTTGATTAGCTTCAGGCAAGAAATTATTAATCACATAAAGTGAAGGCAAAAATGTAAGAATCAAAATAATATTGCCGCTTGTAATCAAATATTTTAAATATGAAACTCCTATATTTTTACTTTTCTTGTCTCTTGCTGGATAAGTGATTCAATGTATTAATCAGTCAACAACAAAAACAAAAAATGAAGAAATTTCAATAAATTTAATTGCTTTATCAAAAGTATTTTTATGATTTGAATACTTTAATAAAATAAAGCTAACAAATCCAAATAGAACAAAAAGTGCAATGAAAACTAAATAAAGTGTTCTAAAAATTCATAATGTAATTCTATGTTTTTTGTTGTTTGTTTTTAAATCGTTATCAACTGTAACAATTATTCCAATTGCTTCAAAAATGTCATTTATTTTCTTATTTTTTATTAAGTTAGTTTCTCTTTCCATAATGGAAAAAATTATAAAACTAAAATCAATATTTTGTTTATAATTAATATACTTATTTTACTTATACATTTATTTTATAGGAGGTTATATGAAGGGTGGCGTAACAATGTGGATAATACTTGGTATCTTAGTTGCTGTTTTAGCCAGCTTCTTTGTATGAAGTGCTGTTAAAGAAAAGATCAAGAAAAAGAAAAGAAAGAAAGAAGAATTACAATTCAAAAAAGATACTGAACAACAATCTAGATTACTTATTTTTGCTTTAAAAATATTAGTTGACAAAAATGAAAAATATTTAAAAGAATTTGAACCATCAATTGGTCAATACAAAATGTCAAACATAATTAATACAGCTCGTGGTTTCTTAGAAAAATATCAAGAAGATGCTTTATTTAAAGAATGTATTTTGAATAATGCTGATGCTAGGGATGAAATTTTTGCTTTTGGTTTTTTAAGAGATACAAGAAGCAATTCGTGAAGTAAAAGATGTGCTGATAAACTTCAATGAATAGAAGAAAAATTTAATTCATATAACATTTCTCTTTATGAAGATGAATATCAAAGAGTGGAAAAAAGAGTTAATGATTATTATGATGCGGAGTTTTTAAAAGATGACAAAGAAGATGAATCTACCAAATAAACTAACAATACTTAGATTGATTTTATTCTTACCTCTTTTAGCTCTTTTTATTACTTATAAGATGATCCAAAATTACATGGATAATTACTATAAAATAGCTGGCCGTTCAATATTATCTGGAATTTTACTTATCTTTTTAGGAGCAATGATTACTGACTATTTTGATGGCAAAATTGCTAGAAAATACAATATGATTACAAGCTTTGGAAAGCTGTGAGATCCTTTAGCTGACAAAATTATTGTCACAAGTACATTAATTTTTTTAGCTGCTTTTAAATTCATTCCTTTATGAATTGTTATAGTTTTTGTAGTTCGTGATTTGATAGTGGATGGTTCTCGTGTAATAATGGCTGAAAAGAAAATTGGGGTTGAGGCTTCAATTTGAGGAAAGATGAAAACCTTGTTTCAAACGCTTGCAATTATTGCTATTTTAATTATTGCTATAAGCTTTGAATATCCTATTGAATTGGTTGAAAAATATGGCCTAACTTTAGTTCAAAGTGATGCTTGTATTTATGCAATTAACACTCTTACAATTGTTGCATTATTCTTCAGTATTTACTCAGGAATATTGTATGTTAAAAAAATAATCCCTTTCCTAGAAATGGTATAAAAAAGCTGCTTTGGTCAAAAAGCAACTTTTTTATATAATTATTTTTTATGTAAGTAAATAATTAGTGCAAGTATCAAAATTACTGTAATTGCAATGATTATTCCAGCTACTAAAATTACAGCTAAAGAAGACAAATATCTTTTCTTCCTATTTGCAATTATATTGGCTTCATTTTTTTTAGCATTATCAATTGCATCTTTAACTAATTTTTCTCTTTGTTCATTAGTTAAATTGTTTAAATCTTCATTCTTAATAATTCTAAATTTTTCAGCATCGTATAGAGTTGTAATCTCTAAGCTGTTTTCTTTTTTGTCATCTTTATTCATGATAATTTTATTTTATCAAAATTGTTTCATTTAACTATGAATTAGCTAAATTTTCAAAAACATCCTTCATATTTTCATTAAACAACAAGATATTTTCATTGTTACTATTTTCATAACGTGAAGTATCTTTATTTAGAATTACTATTCTTTTACCCTGATAAAAATGAATATATGAAGCTGCAGGATAAACACTTAATGAAGTACCTGCAATAATTAATAAATCACTATTTGAAATGGCTTCAGCTGCATCATTAGTTGAATCTTCCATTAGTGGATCTTCATATAAAACTACATCTGGATTTATTACTCCCCCACATTTACAAGTAGGAATTCCATCTTTATCTTTTTGATGAATTAATTGAGCTAAATCATAAAACTTACCACAGAGCAAACAATAATTACGATTAATATTTCCATGTAATTCAATTACATATTTATTACCAGCTTTAATATCCAAGTTATCAATATTTTGTGTAATAACATGGCATTTATTTTTATTGCATCAGCTTGCCACAGTTTCATGAATAATATTTGGTTTAATGTTATCAAAAGCTATATTTGAAAAATAGTATTCATAAAAATCTTTTTTATTTTTTCTTCAAAAACTTCTTGAAAGAATACTTTCAGGATTCATGTTTTTAAATTTTTTAGAATATAAACCATCAGCTGATCTAAAATCAGGAATTCCTGAAGCTGTTGAAACACCAGCTCCACTAAAGAAAACAATATGTTTAGATTCTTTAATTCAATTTTTAAATTTGTCTATTTTTTCATTAAAATTTTGCACCATATACCTCTATTTAATTTTAGCAAAAATATAATTTATGTTTTTGCCAGCTTGACTTCATTTTGCTTCATAGCCTGTCATAACATTATCTTTAGCATATTTGCTTTTATGAAAATCTGTACCATATGCTATTATGTTTCAATCGTTTTCTTTAAAGCTTTCTAGCGAAAATCCATAAAGTTTATCATTGTCTGATTTGAATTTTAGAATAGTATTTTTATCCATAATAAGTTTATATTTTTCCAAAAAATCTTTGTAAGTTAATCTTCTTTTAAAATGTCTAGCCTTAGGTCAAGGATCACTAAAAGTTAATCAAATTGTGCCACAAGTTCCTTTAAAAATGCTTTCTAATTCTTTAGCATCTTCTAAAATAATTTTAAAGTTATTAAGCTTATATTCTTTAGCTCTTCTTACGCTTTTAGCAGCTACAGTAGCAAACTTTTCTAAACCATAAAAATTTATATTTGGATTTAGAGCAGCTAATTGAGTTATCATTTCGCCTTTACCCATGCCAATTTCAATTACATCAGTATTTTTTAAGCTAATAGGATATTGACTTTTAGTAATCAATAAATTAGATTCTTCAAGCTTTTTTGGTGCATCTTTGTCATTTCTTAATCTCATGTTTTCTATTTTACTATATAAAGGAAGTTAAATATTAATTTAATATTTATAAAAAAATAAAATGTTATAATTTGTTATAAGTTGTTAGCGAATGGAGGCAAAAGATAATGGAATTTAAAGACAAAATTTTACGTGCTAGAGCTCTTTTAAACTTAACTCAAGAAGAATTAGCAAAACAACTTGGTGTTTCTTTTAACACCATAAATAGATGGGAAAATGGAAAAGTTAAGCCAACAAAAGTTGCTAAATTGAGATTTGATGAATATTGTAGAAAAAATGGTGTTTTGTTGGAGGTTAGATAATGGAATTTAATGAAGATACAAGAGTAAAATTACCAGCAACGATTCACTTTTTAAAACTTGGTTACAATTATCAAACTGTTAGAAGCCCAGAAATTAATAGAGACAATAACATTTTTGAAGAAAGATTTCATAGAGCAATTGAAAAAATAAATAAGAAAAAATTTGCTAAAGAAGAAATAAATGAATTGATCTCTGATATAAGCAGAAAAATCAAAAATAAGGATTTAGGAAAAGAATTTTATAATCGATTAATTTCGACCGATTACCCTATAAAACTGATAGATTTTGAAAATATTGAGAACAATGATTTTGCAATTGTTAATGAATTAACTTTTAGAAGTAACGCTAATTCTAATTATGATTCATTTAGACCAGATATTACAATTTTAGTTAATGGTATGCCATTAGCATTTTTAGAAGTAAAAGTTAAAAACAACAAAAATGGTATTCAAGCAGAATTTATTAGAATGTGAGAAGATAGATTAAAAGTTGATGAATACAGAAAATTTTTTAATATGTTGCAAATTGTTTCATTTACAAACAATATGGATTATGAAGAATTTAATGATGCTGAGGCTTCACTACTAAAACAAGGCTCTTTCTATACAACACCAAATGGAAAAGACAATACTACTTTTTCAGCTTTTAGAGAAGATGAACCTAACTACCATGCTAACTACTCTTTTGAGGAAATAGACAATGACAAAATAAAAGAAATTTTAATTGATAATAATATTAAAACTTCACATGTTGATAGTGATTGATTCCAAACAAATTTATCAGATACAACTCCTTGCAACCGTTTTATAACTTCAATTTTTGACAAAGAAAGATTTCTATACTTCATTAAATATGGAATGATGTATCTTAGCCAAATAAAAGAAATTAAAAATGAAACTTTAAATAAAGTTGAACAATTGCCAATTAATCAAAAACACATTATGAGATATCCTCAATTTTTCGCTACAAGAGCAATTATCAAAAGATTCAAACGAACTAATAAAAATGGAATTATTTGACATACTCAAGGTTCAGGCAAAACAGCACTAAGTGCTTTTGCTACAAAAGTAATTAAAGATTATTGAAGTAAAAAGAAAATAAATGCAAGATTCTTCTTTATAGTTGATAGATTAGACTTAATGAGACAAGCTTCTGCAGAATTTGCCAATAGAGGTTTCAATGTTGTTAATGTTGATTCAAAAGAAGCATTCGCTAAAGAATTAAATGAGACAATCGATAGAAATAATAATGGTATTGGCACAATTTGTGTTGTAAACATTCATAAATTTATGGATAGTTCAAAAATGCCAGAAGCTAAAAATGATTACAACATAAGAACTCAAAGAATTTTCTTTATTGATGAAGCTCACAGAAGTTATACAATGCATGGTGAATTTTACAAAAACCTTATGACTTGTGATAGAGATGGAATTTACATTGCAATGACTGGTACACCTTTATTAACTAAAAGAGAAAGATCAAATTTAAAATTTGGAGACTACATTCATAAATACTTTTATGATAAATCAATAGCTGATGGTTATACTTTGAGAATTAAAAAAGAACAAATTAAAACTGAAGCTAGAGCTACAATTATTAGAAACTTAAATATTGAAATTGAAAAATTAGAATCAAGAATTATTTATGAATCAGATGATTATGTTAAAAGCGTTAGCCAATATATAGAAAAAGACTTTATTGATTTTAGATTTATTTGCAGAAATGATTTAACAATTGGTGGAATGATTGTCTGTCGTTCAAATGACCAAGCGAAAAAAATTCATGAATGATTTAAAAAGAATTCTAAATTAACTACTGGCCTTGTTTTATCAGACACTGATAATCCATTGCAAGACAACATAAATAAAAATAATCAAAAAACCTTTAAAGAAAGTAATTATCCAGATATTTTAGTTGTTCACTATATGCTTACAACTGGATATGATGTTAAAAGACTTAAGAAGATGTATTTATTAAGAGGACCTAAAGCTCAAAATCTTTTGCAAACTATTTCACGTGTTAATAGACCCTATCGTAGTCCTAATGGCCAAGAATATCAATATGGATATATAGTTGATTTTATTGATATTGAAAAAGAATACAACAATACTATTGATGCTTATCGTAAAGAAATAGAAGAAGAATACGATTTTGAAGAAGAAGACACTTCCCTTTCTGGTTTAATTATTGATAAAACAGATATCAAAAAAAGATATGATGTTTTAAAAAGTGATCTTGAAAAATTTGTTAATAATTACAATTTAGAAACCTTTGTTGACAAAATTAACAATTTTGACAAAGATACTTTACAAAAGATCAAAAAGATTCTTGTTAATATTAAAAACTGTTCAATTGAATTCAAATTATCACAAGCTGATGAATATTCAAAACTCATTAACGATTATGAAATCAATGTGTTTATTAAAGAAGTACAAAAGAAAATTAATTTTTTAAACTTGCGTTCAGATCCTATTGATACAATTCAATTAATAGATAACAAAGAAGTTGTTCAAATCATTTATGATTTTCTTTTAAAAAATATTACTGTTTTAGATTTAGGTAAATTTGATAAAGAAAATAAAATGGAACTTATTAAACAAAAGTTTATGAAAATGCAAAAAGAAATTAAAGAAAACAAAAATACAAGAGATGTTAAATTTATGAATCTTAATGCATTGATTAAGAAATTACTTGATAAATTAGCTGTTGAGGAATTGACTTACGAAGAAGTAGAAGATGAATTAGATTTAATTTACAATTCAACTAAAAAAATAAATGAAGAAAATAAAGAAATATCTAAAGACTTTGGAAATAGTTTGGCTTTTGTCACAACAATGAATATTGTTTTAGCAGAAACTAAATTAGATCGTTCAATTTTAAAGAACTTTTTATTAATGGTTTATGATGAATTAAAAGGCAACATTTCTGACGAAATCCTCATTAAACAAGGCAAAAATGGATTTATTGACTCAACAAAATCTAAAATAACAACTAGATTAATTAAGAAGAATTTATATAAACAAATTAAAGATAATTACGATTATATTTTGAACGAATTATACTTTAATCTGCTTAATTACAAGGAAACACTTTAAGGAGAAAAATGACTAATTTTACAAAACTTGAAAATAATATAAAATCAATAATCGATGATTTGAAGGGATTGTGTTCAACAAATGGTCTTTCAAATACAGCTGGCGAAGAAATAGTTGTTACTTCTGTCTTTTTATATAAATTTCTAAATGATAAATTCATTTCAAATTTAAAAAAATTTGCAAAAGAATTAAATGTTCCTTTTGAAGAAATAATTAAAAATTCAAACTATATGGATGGATTTTATGATGCTTATCCACAAGATGTTGCATTCAAATATGAAGATACAATTGATTATTTAGTTGGATACATTAATGAAGATGATTTTTATCAAAAATTTGACGATACATTAATTAGAATTTCAGATTATAAACAAAATGAAGCTTTTAATGTTGATACTGCAGAAGGAACTAAAAAGCCACTTTTTGTTCGTCTTAGTACTTTTGTTGAACCTTCAAAAAGAAATAACTTCACAAAAAATGTTTTTTCTTACATTACAAGAGATAGATTTGATTTTGGTGAAGCTTTTAATAATAACTATGATTTCTTTTCAACTATTTTTGAATACCTAATTTCAGATTACAACATTGCAAGTGGTAAATATGCTGAATACTTTACACCTCAAACATTATCAAAAGCTATTGGTGAAATATTAGTTAAAATGTCTCCAATTGAAGATAAAATTTATGAAATATATGACCCAAGTGCAGGTTCTGGATCGCTTGTGTTGCATTTAGCAAATGAACTTGGAGAAGGTAAATTTGGAAATAAGGCTCGTGTTTACACTCAAGATATTTCACAAAAATCATCAAGATTTTTAAGAATAAATATGTTACTTAATGGTTTAAAAGAATCACTAGATAACATAATTGAAGGCGATACCTTACTTACCCCAACACACTATAAAAAAGCAGGAGACGCAACAACAGGTCTTAAACAATTTGATTTTATTACATCTAATCCACCATTCAAAACTGACTTCTCTTCTACTAGAGATAACATTGAATTAATGTGACAAAATACAGAAAGATTTTTTGCTGGTATTCCTAAAGTTCCCAAAACTAAAAAAGATTCAATGGCTATTTATCTTTTATTTATTCAACATATTCTTTATTCATTAAAAGAAGGTGGTAAAGCCGCTATAGTTGTACCTACAGGGTTTATTACAGCACAAAGTGGAATTGAAAAAACTATTAGACAATATATTATTGATAGAAAATGACTAAAGGGTGTGATTTCAATGCCATCAAATATTTTTGCTAATACTGGAACTAACGTATCCGTTTTATTTATAGATAAAACAAATACTAATGGTGAAGTGTTATTAATGGACGCTTCAAAAATGGGTCATAAAGAAAAAGTTAAAGATCTTCAAAAAACAGTTTTAACTCATGATGAATTAAACAAAATAGTTAATGATTTTGTTAATCATAAAGTTGAAGATGATTTTACAATTTCAGTTACCTATGATCAAATAAAAGAAAGAAACTATTCATTATCAGCTGGTCAATATTTTGAAGTAAAAATTGAATATGTTGATATTACTCAAGAAGAATTTGACAAAATGATTGATGACTTTAATAAAGAATTAGAACAATTTACAAAAGATACAGAAGAATTCCAAAAAGAGATGTCTAAAACATTGAAGGAGTTGAAGCTAAAATAAATTAATAAGGATAAATATGGAATATAAATTAAAAGAATTAGGTATATTTGAAACTGGAAGCACATTAATCAAAAAAATTGGAAATTTTCCTGCTTTTGGAGGCAATGGCATTATTACATATGTGAATAAATGAAATGTTGATGAAGATGCAATAATTATTGGTAGAGTTGGAGCGAATTGTGGTTGTGTAAATATCACAAACAAAAAAAGTTTTGTTACAGATAATGCTTTAATATTTAAACCTAAAGAAAAAAATATGGCAAGATTTTATTTTTATTTTCTTTTACATTTAAACTTAAATAAATTTCATATAGGTTCTTCACAGCCTTTATTAACTCAAGGAATTTTAGGAAACATAAAAATAAATATTCCTAGTTTAAATAAGTGCCAAAAAATATCAAAAATATTAGATAACATAGATAATCAAATTGAGCGAAATAATTCTATGGTTCAAAAGTTACAGTGTTTTGAGCAAGCATTAATTTTCTCAAAAAAGTGAGGGGCAAATTATGCTTGTTAAATTGAAAGATATTGTAACTTTCATTAATGGAAGAGCCTATTCTCAACCTGAATTACAAGACAAGGGAAAATATAGAATAGTGCGAGTAGGAAATTTTTCTGGTAAAAATGAATGATTTTATTCAGATATGGAATTGAATGAAGATAAATATTGTGAAAATGGCGATTTGTTATATAAATGAGCTTGTAATTTCGGCCCTGAAATTTGGAAATCTGAAAAAACAATATTTCATTATCATATATGAAAAATAAAATGAGATGAAAAGCGCGTCGATAAAATGTTTCTTTATTATCTTTTAATGTATATGACACCTTATTGGCTTTCATCAACTAATGGATCAATAATGATTCATATTACAAAAGAAACAATGGAAGAAAAAATTGTTGATTTGCCTCCACTAAAAACTCAAAAAAAGATTTCTAAAATATTAGAAAATTTAGACAAGCAGATTGAAAAGAATTTGCATATAGTCAAAAGATTACAAGTTATGGGACAGGCTATTTTTGATATGTTTTTGAATAATGCGAAAGATTATGAAAATATAGAAAGTTTGTGCAAAATAATTTGGGGTCAGTGTCCAAAAGGAAACAACATTTTAAGCGAAAACGTATCAAATAATTTAATGCTTTATGCATCAGGAGCAGGTGATTTAGAGAATAATAAAATATTAATTTCTCCTAAAGCTTTTACTGATAAACCAATTAAAATAATAGATAATAGGACAATTTGTATGTCTATTGCGGGAACTGTTGGTAAAATTGGAATAAGTGATAAAAATATTGCAATTGGGAGAGCTATGGTTGGATTTTATAATGAAAAAAAATTTGGACTAATTTATTTTATTCTAAATAAATATAGTTCATTTTTAAAAAGACAATCAATTGGAGCTATACAAAAAATTATTAACAAAAATCATCTCAATATTGTGAATGTTCCAATATTAACAAACGAAAAAAACAATTTATTAAACGAATTAATAACTAAATGCATGAAATTAGAAAAAAATACTTTAAGTTTGATAAAGCTTAAAGAAAAGTTGATACCTTTATTAATAAATGGCCAAATAGAAATAAATTAGCCCTATCTATTGATAGGACTTTTTTAAATAATTTGTTGATTAATCAATAAAGGTAATAGCTTATTTTTGAATTTTATTAATTTCTTAGTCATTAAACTGATTTGACTTATTTTAGCATATATATTTTGAGTTAAATTATTAAATATGTCCAAAACATTTTTTGGTGGTAAAACAATTTTTAAATTTTCTATAAAATTTTTATTTATATGTTTTTGACAGTTCCCAGTAGAAGATCTTATTAAAAACTCAATTTTATTCTTTAAAAAAGGATATAAATATCCTATTTTCAATAACTCATTTTCTTCTATTCCTACTATAGATTGATTTATACATGAATCAATTGCAAGATAACTGACCCTTATATTTCCTGTTATAGAAATAACTACAGTACCTTTTTTCAATAATTTAGTGTTTGAATTTTTTAAACCTTTTTCATTTATTGTTTTTTCGCTATCTATTATTGGAAAATTGGCTACCTCACCAGAATTTAGTCAATTTATCTCGCCATTTCAATATTCTCTATTTTTTGTGCTAGGGGTTCCTCCAAGAAAAATTTTACAAATTTCAGCAATTTTTTTAACTTCTCAATTTTCAGGAATTTTTAAATTTAAATCTTCATTGTATTTAAATTTATTATCTTTTTTAAAATGCTCAAATTGTAAAAATCACCTATTAAAAATAGCCTGTCCCATAACTTGTAACTTTTGAAACTAGCTATTTTTTAGTGCTTTTTTAGCTCTAACTAATGTTGATTTACTAATACCAGTTAAAAGTGAAACTTGGCGATAGGAAAAGGAATCTAAAAGCAAAATTGCTTCACTTAAAAGATCTTCAGTATAAACTTTTGGTCTACCTTCTTTATAATCTGTTCTTAGTCTTGCTATCTCTTTACCATTTTGTGTTCTTTCTACTATCATGTTTCTTTCTAATTCAGCAACTGCTAACATAGTTGTTAAGAAAAAACGACCCATTGGAGTGTCCTCTAATAAGCCTATATTTAAAATATGTATTGCTACTTTCTTTTTAAATAAAGCTTCAACTACTTTAATACCTTCAACTGTATTTCTAGCAAATCTATCTAATTTAGAAACTACTAGAATATCATCTTTTTCTAATTTAGTTAATAGATCATTAAATAGTGGTCTATTTAATTTTGTTCCTGTAAATTGTTCATTAATTATTATTGCGTTTGGATATTTATTCAATATTTCATTTTGTTGTATTTCAAAACCATATCCATCAATTTGTTTTTTTGTGCTAACTCTTGTATAACCATAAATCATTTTTTTACCTCGCTTTGCAATTAACACATAATTAAATAGTTAATATTAATTCTTAATTTTTGAGCTCAAAAAGCAATAAATAATATTTTAAATAATTTTTGGCACTCTCACCCTTAAAGTGCTAAAATTATGATATAATTGATTTTCTATAGGAGGTGAAATTATGGATTTTAATTTAGATGACTTATTTGGTTCTAATGACAATAAAAGTAAAAAATCAAGTGATCCATTAAAAATTTATGGAAGAAACTTAACTGATTTAGCTGCTCGGAATGAATTAGATCCTGTTATTAATCGTGATGATGAAATCAGAAGAATGATTCGCATTTTGAGTAGGAAAACAAAAAACAACCCAGTATTAGTCGGCGAACCAGGTGTTGGTAAAACAGCTATTGTTGAAGGATTGGCTAGAAAAATAGTTGAGGGTCAAGTACCAGAAAACTTAAAAGGTAAAGATATTTTTGAACTTGACTTAGCTGCTTTAATAGCTGGCGCTTCATATCAAGGACAATTTGAAAAGAGATTAAAAGACGTACTTAAAAGAATTGAAGAATCTAATGGTGACATTATAGTTTTCATTGATGAAATTCACATGCTTATTGGCACAGGTCGAAATGCTGAAGGTGGTATGGATGCTGCTAACATCATTAAACCTTTAATGGCCAGAGGTAAAATGCATTTAATTGGTGCTACAACTTTTGATGAATATAGAAAATATATAGAAAAAGATGCGGCTCTTGAAAGAAGAATGCAAAGAGTGGATGTATTAGAGCCTTCAATTGAAGATACTATTACAATTCTTCGTGGTATCAAAGACCGTTTTGAAAATTACCACAATGTAAAAATTCAAGATGATGCTTTAGTAGCTGCTGCTCGTCTTTCTTCAAGATATATTTCTGATAGATTTTTACCAGATAAAGCTATTGACTTAGTTGATGAAGCTGCTGCAACTATTAAAACAGAAATAAACTTTCAACCTGAAGAATTAGAAAAAGCTAAACAAAAAATGGCTAGATTGAAAATGGAAAAAATAGCCATTTCTAGTGAAGACAAGAAAAAACATGCAGTTAGACTTAAAGAAATTGATGCCGAAATAAAAGATATTGAAAAAACAATAAAACAATTAGAAGATAAATGAAATTTTGAAAAACAAAGACTTGAAGAATTGTCTAATTTAAAAAGTAAATTAGATGACGCAAGACATAGATTAAATATTTACCAAAAAGAAACAGATTATGAAAAAGCTTCAAAAATTTTGTATGATGAAATTCCAAGCATTAAGAAACAAATCGAAGAAACAGAAAAAGAAATAGCAGAATCAGGTAACACAATTGTTAAAGATAGTGTTACAAGTGAAGAAATTGCTAACATTGTTTCGAAATGAACTAAAATTCCAATTTCAAAATTACTTGAAAGTGATAAAGAAAAATTGTTAAATCTTGAAAACGATTTAAAGAAAAGAATAAAAGGGCAAGATGCTGCCATTAGATTAGTTGCTCAAGCTGTATTAAGAGCTAAAGCAAATATTAATGATCCTAATAGGCCTTTAGCAAGTTTCTTATTTACAGGACCTACTGGTGTTGGTAAAACAGAATTAGCAAGAGCTCTTGCATATTCTCTTTTTGATAGTGAAAAACAAATGATTCGTTTAGACATGTCTGAATACATGGAAAAACATAGTGTTTCTAAAATCATTGGTGCTCCTCCAGGATATGTTGGATATGACAGCGGTGGATCATTAACTGAAAAAATTAGAAAGAATCCTTACACAATTCTTTTATTCGATGAAATCGAAAAAGCTGACCGCGATGTTCTTAACATTCTTTTACAAATGATGGATAATGGGGCTATTACAGACTCAAAAGGCCGTGTAATTAACTGTCGTAATTTAATTATAATTATGACAAGTAATTTAGGCTCACAAGAAATACTTAAACATTTAGATCAAATGCCAAGTATTAAAGCTGAATTGCTTAAATTCTTGAGTCCTGAATTTGTAAACAGAATTGATGAAATTGTCAAATTTAATCCATTAACTAAAGATGTAATTAATGAAATTGTTGAACTTGAACTTCAAAAATTAGCTACTAGAATGAAAGATGTTAAAGATGTAAAAATATCATTCACAAGCAAAACTGTTGACTTTGTAGGTTCTTCTTCATATGATGAAAACTTTGGGGCAAGGCCTATTAAAAGATTTATCCAAAATAAAATTGAAAGCTTATTAGCTTACAAGATTATTGATGGCACCATAAGAAGCGGAAAAGCCTATAAAATAGACGTTTTCGCCGGAAACTTTGTTGTTAAAGAAGCCTAAATTTAAATAAGTATTGTTACAAAACAGTACTTATTTTTTGATCAAAAAAAGACCTTTAAAAGGTCTTAATTTTTAAAATAATTTCATTTGGTCAGTATCGTCAAGACCTTTAAAAATGTTAAGTTTATCCATTTTTTCAATTAATGTTTTATTAACTGAAGTACGGCGAATAACATCTTCTTTTGAAGTAAAATCTTTAGCATCTCTAGCTTCTACTAAAGATACTGCAGCTGCATCACCTAATCCATCAATTGATTTAAAAGGAGGAATTAAACAACCGTGTTTATTGTCAATAATTCATTCATGTGCTGCCGATTTTTTCAAGTCAATATTAGCAATATAGAAACCACGAGCATAAAGTTCTTGAGCTATTTCAAATACTGGAATTAAGTCATCATCTTTAACTTTTCTTTCATTCTTTGGAATGTTATTCAATTGATTCAATCTGTCTGTAACTTTATGTCCACCTTTAATATCAATCATACTTTCAATTTCAACCGCTTTAGCATGGGTTGCAAAATAACAAGCATAGTGAGCTAAAGGATAATAAAGTTTAAATCAAGCAATTCATCAAGCCATTAAAACATAAGCGGTAGCATGGGCTTTGGGGAACATGTACTTGATTTTCTTCATACTTTCAATCATTCAGTCAGCCACTTTATATTCTTTTAATCTCTTAACTTCATCATCAGTTAAACCTTTACCTTTACGAACTTTTTCCATAATATTGAAAGCAAATAAAGGTTCAACTTTTTTCTTTAAAAGAAATTGCATAATGTCGTCACGACAACAAATAACATCAGTTATTTCAAAATTCTTTTTGCTTACTAATTCTTGAGCATTGTTCAACCAAACATTTTCACCATGAGAAAGTCCAGCTAAAGAAATAAGGTTAGCAAATGATTTTGGTTTAGCTTCTTTTAACATTTGACGAACAAAACTTGTTCCGAATTCAGGCAAACCTAATGCCCCTGTTGTTTCATTATTAATTTGTTCAGGTTTAATATTCATAGCTTTAGTACTTGCAAATAAACTTAAAACTTTAGGATCATTTTTTGGAATGTCATCAATATTAATATTGGTATATTTTTTAAGCAATCTAATAATTGTTGGATTATCATGACCAAGTAAGTCAAGTTTTAAAATGTTGTCATGAATTGAATGGAAGTCGAAGTGACTTGTTAATCAAGAAGAATCAGGTTCATTGGCTGGATAGTTTATTGGGGTAAAATCATAGACATCAAATTCTTTTGGAATAATAAGAATACCACCTGGGTGTTGGCCTGTAGTTCTTTTCAATTTTTCTATTTTATGGGCTAAAAAGTCAATAAATGGTTCTGAATAATTACGCATTGCTTCTTCTGTAAATGCTTTAACATGTCCATATGAAGTTTTAAGAGCAACTGTTGATATGGTACCAGCTTTTAAAGTGTGAGTATTACCAAAAATACGTTTAACTTCATTGTGAATATCACCTTGAATATCGCCTGAGAAGTTAAGGTCAATATCAGGAACTTTATCAGCTTTAAAACCTAAGAAGGTTTCAAAAGGAATTCCTTGTCCATCAGCTTCTAATAAAATATTACATTTAGGGCAATTTTTGTTAGGTAAGTCATATCCACTTGTTATTTTTTCATCTTTTGACAATTCAAAATATTTACATTTGTTGCAAAGATAATGAGGAGGTAAGGGATTAACTTCAGTAATACCTGCAAGGGTTGCAACAAATGAGCTACCTACAGATCCCCGACTACCAACAACAAAACCATTGTCAGTTGATTTTTGAATTAAGATATGACTAATTCAATAAATAACACTAAAACCATAATCAATAATAGGATTTAATTCGGCTTCAATTCTTTCTTCAACTATTTTTGGTAATTTTTCACCATATTTTTTGTATGCTGTTTCATAAACTAAATCATGTAGTTTTTTACCGCTGTCTTCAAACTTAGGTGTATAAAGTTTATCTTTGATAACTTGAATTTTGTCGCACATGTCTGCTATTTTATTTGTGTTGTTTATAACTATGTCTTCAATTAATTGTTTGTCTTTTAAAAAGGCAAATTGTTCAAGCATTTCTTCAGTGGTTAAATAGTCTTGATTTGGAATTGGAATATTTCCGTTTTTAGCTTGTTCATAGTTGAATAAGTAATGACGAGAGTTTTTAATTCCTTTAGCATAAACTAAAACCTCATAAGCTACTTTGTCATATAAATTTAGATACTTAACATCAGCTGTAGCAACCACTATTTTTTTAGCTTTTATTGCTTCTTTAATAATTTCTTTTAAAGAAATTTCTAATTGTTCTTGAGTAATAAATTCATTATCAATTCAATGGCTAAACACTTGAGGTGCTGGCACTTCAATATAGTCATAAAGTTTTAATTCTTTAATGAATTCTTCATGTGAAGAATAAAAATAAACATCAAGCAATCTACTTTTTAGTGTTCCTGAACCTAATAAAAGATTTTCACTATGAGCTGCTAAATCTTCCATAAATGTTTTAGGTCCATTGTATAAGTTTTTAGTTAAAGCTGTTGTTAAATAACCATATTGTTCTTTTAAACCTTCATAGTTTTTAGCTATTGTTGAAACTTCATATGAAAAGGTTTTATTATAAAGATCATCTTTAATATATGAATTAAGTTGATTAAAGGTTAAAATACCTTTTTTACCTAATTCGTCCATTAAAGCCACTCAAACTTCAGCTAAAACTTTCGCATCATAATCACCACGGTGAGCAACTGTAGGATCATATTCAACACCAACACGAGTAGCAACATCTTCAAGTTTATGTTTTTTGTTTTGAGGGAAAACAATTCTTGAAACAATAAGTGTGTCAATTACTGTTACATTTGGAAATTCTATTTTAGCTTGTCTAAATTTTTCTTTTAAGAAGTTGAAGTCAAATCTAGCATTGTGTGCTAAAGCAATTTTGCCATTTAAGTCATTATAAATTTTTTGCAAAGCTTCTTTTAATTCCAAACCACTTTTTTCAATCATTGCATTAGTAATACCTGTCAAGTTTTCTGTAAAAGGTTTAATTGGTTCTTGACATTTAATGAAAAATTGTGTTTTTCTCCCATACTTCATGTTTGAGTGAATTGGCAATGAACCATATTCAATAATTTCGGCAAAACGAGGACAAAGTCCAGTGGTTTCAATATCGAATGAAACATATTCTTGACCTTTAATTTCGCCTTCAGGTATGTTTCCTAAAATAGATTTATTACTTTTGTTTATAACTGAAAACGCAGTTCCAAAAAGAGGTTTAACTTTTGATTTTTTAGCTGCATTATAAAATTCAGGATAACCTTGAGCACCATCAACGTCCATAATTGCAACAGCAGAATGATTCAATTTTTCAGCAAAAGCCACAATGTCTTTAGGAAAAATTAATCCATCCATTGTGTTCATTTTTGAGCTAACATGTAACTCAACTCTTTTTTTAGCTATGTCTTTATTTTCTTTCTTTTCTGTTAGTAAACTTTTTGCTTTTTCATAGAAATCTAAGAAGACAAATTTGTTTCTAGCACCAAAAGTACTTTCAGAAACTGTTCCATAAATTGTTACTCATTTGTTGTCTTCAAGCTCATTTATTTGTTCAGGTTCTAATAATGTTGTTCTTATTGTTTTTACTTCTACAGCATCTTTATAGTTGGTTAGAAAAAATGTGTAAATGTGTTTACCATTTTTACTTAAAGCATAGTCTTGAGAATAAATCATTCCAGTAAATGAAACAGCAGTTTTATCATCTAACGAAGATATGTCTTGGAGCTCAACTTTTTCATAGCTTTTTCTTCTTCATCTATTCGAATTATTTGGAGTTGTAACTCTTTCATTTTCTTTGGTAAGTTTTTCTTGGTTTTCTTGTCATTTTTTCATAAATTCTAGAGTTCTTTTGTTTTCTTCCTCAATAACACCGTTGTCAACTGAAACCATGGTTTTTTGTTCAAAATTAATTTTTAAATCTTTTCAACCGTATTTATTTAAAGTTTTTTGAAGGTTTTTAAAAACGGGGTTAAACTCTTCATATTCTTTAGCTGAAATAGTTGTAATTGTTGCTACAAATTGATCATTAAATTTAAGTTTTTTAACAATTGGCAAATTTCTCAATTTGATGAATTTGGCTTTTTCATAGGTTAATTCGTTAATAAAATCTATTAAATTTTCTTTTTTATATTGTGAAATATCAAAAGTAATTTTACCTTTGATGTTATAAACTTTATTATTTTTTAAACTAGAAAGAAACGAAAAAAATTCCTTTGCACTGATTGAATTTGTAAATTTAAATGACACATTTAATTGGTCTTTAACACTGTCATATTCGACATCGTCTAATTCAACTTTATCCAATCCAGGTGTCACTTTTACATTAATATCTTTACAAAATGTAATGAAATTTTTATTTTCTATTTTTCTATTTTCTTTAGGCATATTATTACTATTATACATATATTTTTTTATAAAAATTTAATTATGTAAATTAATTTATTTTAATAATTTTTTAGCCTATTTTTAAGCTTTTTTATGTTAAAAATTAACAAGAAAAATACCACTAATTTTGTGGTATTAAACCAGGACAAAAGAATTAGACTTAAATCCCAAAAAGATTTAAGTCTTTTTGTACTAGTTTAAAGCATAAAATGAAGTTTTATGGGAAAACATTTAAATACTGAAAAATGGATAGAAATTTTTATATTTTTTAAAGAATATTTAGACTATAAAATTACAAAGATGGAATTTAAAATGAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGATGGCTAATGCCCTCACTTTTATTTGTCCTATCGGAAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGGATTAGCCTTTAAAGCTAATCATTTTTATTATTCTGGATAATTTGATATTTAATAAATGTGTCTTTAAACATATTAATATCAAAATATGTTAATTTATCAGGCTTAGTATTTTCAAAATCGAAGATTGAATTTGCATATACTAATTTTCTTTGCTTTTTAGGAGATAAGTTTTCAAAATTTTCTGGATATTTAATATAAAAATCGACTGATCTGAAAAAGAATGAAACTAACGACTTTTCATTTGGAAGGGAGTTATAGTGATATTTTTGGCCTAATGAAAAATCAAATTGTGTTTTGTTAGTTTCTGAATTAATCTTCTTTTCATAAAGATTTTCGTCATTTAAATTATTGTTGAATTTCAATCATTTGGTTGTTTTTACAAATATGTTTATCTGTATAAGGATAAAACAAATTACTAAAAAAGTGCTACCGCCGAATATAATTCAATAGTATGCAAGCGAATCATAGTATTTGATATTAGGCTTTCATAATAATCCTACTAGCAAAGCAATAAGTATTTCTATTAACAGTCAGCCCAATGAACCTAACATATAGAATAATTGATATTTTTGTTGGTTTTTGATAACAAAATCTTGAAGTTTTATTTCATTACTTATGCAATTAGGTCTTACTGTTAGACAAAAATATAAGATTTTTTTAAAGGTATTTTGTTTGACTAAAATTGAATCTATGAACAAAGGCATAAGTGTAATAAAACATATAATAATCACTAATCAAATCATTTTTAACTTTCTAAACAATGAATAATATATTATTCAATAATTTTATAAGCTTTTGCTTCATTTTTGAACATATTAATATTTAGCGGCTCAAATTTATTATCAAATGCTGTATCATCGTAATTGAGAACCATAGTCATATAAATAAAAAGTTTTTGCTTTTCGAATGAGTATGACTTGAATTTTTGAGTATTAAAAGTTGAAAAATAAAGTGCATTAAAACCTTGTCTTTTAAAATAACATTTATTTTTAGATGGATACAATTGTTCAACATCTGTGTTTATGTCAATTGATTTTTCGATTAAAGAACTATCTGGAAGCATATTATTAAACTTTCTTCACTTTTTTTGTTTTATATATCATAGTAAGCATACAACAATATATCACAAGGGCAATGGTACATAAAATATTATGCCAAGTGTTACAAACATTGTAAAAAATGATTCCTTTTCATATACTTGTAGAAAAACCATTAAAACTAAGCTTTCTAGCATTAATGCGATTGCAAGTAAAGCATAAATTAATGGAATTTTAGACATCAGACAATAATGATTTACTAATGAATTTTGAACTCTTAATTTTCTTTTCATGCAATATTTATTTACACTACCAAAAGCATATATATATCATCTAATTTTAGGAAACAAAATAATGAATGGCGTAAGATTAATAAAAGCAATTCAAAAACTAAAAAAGATAATTGAAAAAATTAAAAAATTACTCATATGTGATTATCCTAAATTATCATATAAAGAATTATTAGCTTTTTTACGCAAATATGTTCCTTTTTTAGGGTCATTAACTCTTTGTAAACTTCACAAACTTAATTCAAAATCAGAAAGCTCTAGCATTTCATTAATAGTTTTTATTTCGTTGGTTTCTAAATTTTTAAAAATAAGGTTTTTTCCACCAGTACCTCCCATATAATAAGGACCAGTTTGTTCGCTTTCATGTATAACAACTCATCCTATTTTTTTAATATCTTTTATTTTCTTTGAAAAATCACTAATTAAATCTGATGCCTTTTTAGCACATTCATTAAAATAATCTAATTCTTTTGAAGCGGTGTAAACATCATAAAATGATCCAAGAGTTTGTAATGCTGAACCAACAACACCAGTAGCCATAGTTGCTATTGATGGCGCAGCTTGTGATAATTTAAGCATTTCATATACTCTAGCAACACGATTTAATGCCATAGGAATACTTGAAACCATACCTCATATTGGTATGCTATAATTTGACGATTGAAATAGCTCAATAAATTTTTGAGAATTTTTTAGAGTCTCGTTTAAATAATATATTCTATCAGATAATGTAATTGCGGCAATTGATAGCAAAGATGCTGACGCTGATGCTACTGATGCAGCAATTGCACATGGTGGACAGAAAAAGCTAGCTATTCATAATCCAGTTGCTGATGCTGCTGATATGGCAGATAATGAAACACAAGCGATTTTTCATTTTTGCAAGAACGCAATATGATCTATTGTTTTTTTAATTGAATCATTAAGGTTAATTAAATGATTATTCAATAACTCATAATCTTTTAAAATAAATTTAGATGAAATTGTACCGTCATCATTTATTTTTCAATCTTTATTTTCAAAAGACGAATTGACTCTTTTTATCATTGAATTGATTTCTATTTTTGATTTTTTTTCGCTTTTTTTAAAAGTTAAAGATTGTTCTTTTATTTTTTTAATTAATTCAGTTTTAAAAATTTCTCTTTTGTCTCTTAGTTTATTTAATAATTCATCTTTATTTTTTATACTAGATTGGCTAATTTTAGATATAAACAAATCATAAATTTTATCTAATTTTATTTCATCAATGGAATTTATAACTTTTTTAGTTGAATTTTTGTTATTGATAATATCATTTGAGATATAGTTAAAAATTTCAATTTCATCGTTTGTAAATCGAGAAAATAATTCCAAAATTTTTTGTTGCTCTGAATTTAAATTTTCATTTGATGCAACAATGACTGATGGTGTTATGTTAGTTACATTAATTGTATTTAGTAAAGTAGATAATTTCATAAATTATATTAGCAAAATGCCACTCTCCATTTTATTATAAGGTTTTTAGATGCTACGACATTTCATATTTATAAATAATAAGACAATGATCCAAAACAAACATTAGATATTTATATAACTGCATTGGGAAATTGTTATTAATATTTTTAATAAATATTACTATAAAACAGCACTAAAAGTATTATAGAATATATATAGTCCTTAACTTTAACTTCCTAAATTAATAACTGTATATATTCTTAATTAGATTATAACATGAAAAATTAAACTAGGTGTAATAATTGATTAGTTTCTGTTTTAAATACTCATTTATTAGATTGTTTAAATGCTAATACAATTTTATAATTGTGATTATTCCCATATGCTTTAAATTAATAAAATGATTGCTGGGTTTAACTTGTATTAAATAAGTAATATTATCGTAGCAATTAAATCAATGCCTATAATCGCGTCTATTAATATGTCTGAGACTTTTCTTTATAAGTCAATAAATCTAATAATTCCTTTTCTCTGTTGTAATTAATAAAACCTTGATAAAAAATAATTTGATACATATTTAAAAAATTTGTTTTGGTGTTGGCTTTATGCTTACTTAGATAATGACCTAATTTAGCACAAAAAACGCCACTATATGCCTTTTGGAACTCTGTAAAGCTTTTATAATTTTTAGTCCACAAAATCTTTTTAATATTTATATTTAAATCTTTATAGGTAGGAACTTTTAACATTGAAAATAATTGTAAAATAAAAAACAATATTAACTGTTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAATTTTTTTTATAAAAAAGTCACTCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCAATGTTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTCATTGAATTTTGAACTGAAAAGATTTATTAAGAAAATTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTGTAGGCAAGAGTAAAGGCACACATTGTCATTTCCTTAGTTTTACCGAAAGAGTCTCTAGATATGGATTTTTGGTAAAAATCCATGAAATTTAAATTTGTGTTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATACGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAAACTAATTTTGATGATATTATTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCAATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGATACCACTAATTTTGTGGTATTTTAAAATTATATTAAGGTTTTATAACTGCACGGGGAGTATAGTCCAAATAATAAATTAAAAATTCGTCAATAGCATTTTTTCTATTTTTATAATATGTCGATTTTGAAAAATAATCCTGGTATCATAACTTGTTTTCAGATTGTTTCATAAACTCATTTTTAATGATCAGTTGACTTACACCGCTTAAGCAATTGTATACTCTTTTGAATTTGTCTGAAAAGTAATTATTAGATCTTGAATTTTCATTCTTCTTGTAATATTTTTCTATAAAATCTTCTTGACGATTTTTCAAGAATATGGCTTTTTCCATATTAATTATTGTTTCTACACTACGATACTTTTGTAAATTACTCATTTGGTATTGTGAAGCAAATTGCGAAATAGTACTTGTCATATTACCTTTCTTTTTTAAATCAGGTAAATAAAAGTATATTGCTAGTAAGCTCTTGCAAAGATTACATATCTATTGGTTTCTTTACAACATTTTTCAAAAATACATTTGTGTTTTTGATTTGGTTTATCAAACTTTTTAGGTAAGCAACGAGTTGAAGCGCCAGTTTCATTTTTGATTATTTCTTCAGCCACATCTAAACAACAAAATGGGGCTATAACAAATTTATGATTTTTAATTTGTTTTTTGAAATCTTCGTAGTTATCTACAAAGACTGTATTTTCATTTAGTCTTTTTTCAGCTTGTAAGTACAAGTTATCGTGGATTTGATTTAAAAGAGTTTCAACTTGATCCTTAACTTCTGATTGTTTTATATTAATCTTTTCAAGGGTGTCTCTTCTAACAATTGTCACACTTTTATTTTCAATGTCTTTAGGTCCAACTTCAATTCTAAGAGGAACACCTTGAATTTCAGAATTTGAGGCTTTAAATCCTGGACCTTTGTCACTTGAATCAAGTCTTACTCTAAATTTTCTACTTAGGTCTTTGTAAAGATTATCACAGTATGTATGAACTTCTTTATTTTTATTTGCAAAAAGCTCTAAAACATCTATTTGAATTGGAGCTACTCGAGGTGGAATAATAATTCCTCTATTGTCTCCATGAGTCATTATAATAGCTCCAAGCAGACGGGTTGAAACACCCCATGAAGTTTGGTAAGCATAATCTAATTCATTGTTTTCAGTTTTGAATTGAATTTCAAATTGTTTTGAAAAGTTTTGAGCTAAATAATGGCTTGTTCCTGCTTGTAAAGCACGACCATCCTTCATCATTGCTTCAACTGTATAAGTTGAACATGCGCCAGCAAATTTTTCTTTTGGCGTTTTCTTACCAATAATTGTTGGTATTGCTAAAAAGTTTTTTAAAAATTTTGAATAAGTTGAAATCATTCCTTTTGTAAATTTTCTAGCTTCAAGCGGATTACTATGACATGTATGTCCTTCTTGTCATAAGAATTCTCTACTTCTTAAAAATGGGTTGGTTGTTTTTTCTCATCTAACAACATTAGCTCATTGGTTATAAATCATAGGTAAGTCTTTGTAAGACTCAATTGAGTTTTTAAATAAATCAGCAAATAAAACTTCTGATGTTGGTCTAATAAATACTTTTTCATCTAAATCCTTGTCGCCAACTTTAGTAATTGTTGCTAATTCTGGATTAAATCCTGCTATGTGATCTTTTTCTTTGTTAAACAATTTTTCAGGTATTAAAAGTGGCAAATATACATTTTGCACACCTTTTTCTCTAAAACATTCATTTAAATTTTTTTGAATTTGTTCTCAAATTCCATATGAATTGGGTTTAAATACAATTGTTCCTTTTACAGGCCCATAAGCAATTAAATTCCCTTGTTTAACAACATCTGTATATCACTTAGCAAAATCTTGTTCTAATGGAGTAATTTTTTCTAGTTCTTTCATAATTTTAATTATATTAAAAAGTTAATTTTTGGCAAGTAAAAAGGTTGAAAAACTAACTTTTTTAAATTTTTGGTGTTTTTTAAATTAAAAAACTTGCTTATTAATTTAGCAAGCTTCTTATATATTATTTTTTATTTCTTTTCTTTGTGATTTTGGTGTTTTCTACATTTTGAACAATATTTGCTTAATTCAACTTTTTCTGGTGTATTTTTCTTGTTCTTTTTACTAATGTAGTTTTCCATTTTACATGATTCGCAAACTAATGTAAAACCGTCTCTTGGCATATTAATCCTCCTAAAAGTTCAAAATTCTTTTTATATTATATAAAGAAATTAGTTTTTTGAAAATATTTTATTTCATAAATTAATTTACATGTATGTAATCTAAACCTAAACTTTGACGATATGCAGCAATTTTTTTAATACTTTGATCAAAATCTCTTGCAGTATTTCCAGTATTTTTTATTTGATCTCAAGTTAATAAATAATAAGTAAATCTATGTGGCATCATTTTTTTACCTTCAAAATAATTTTTCATTGAAGGGCCCATTGTTGGATCATTTCAAGTTGGATCTAAATATAATTCTCGCCCTCCAAGTCTTACCATATTTCAAGCATGACCTTCTTGCCCACTTGAATTAGCAGCTTGACCGGTTACATATCACACAGGAATATTTATTAATGTTAGAAATCTTGAAAATGTTCTTGCATATCCTTCGCAAACTATTCTTCTTTTTGGTTTAGTAAAATATGAATATGGACTACGACATTGACTTCGTATTGGTTCATTATTTGATAAAGCTAACTCTCCATCAGTCAAATATTCATTATTGTCAATCACTCAATTATGTATTGCTCTTATTTTTTGTTCATCAGTCATTTTTTTAGAAATTATTTTATAAATTACTTCTGTCATCAATGAATTTAGTTTAACTTCTTCAGAAGCACTATCTGTTCTATATCTAAATTCTGATTCTTTTATATTTATTTTTTGACGCGTGTAAGTAATACGTTCAAGGATTTTGTCATTAAAATAATTGCTACCACCAGTTATGTCATATCAAGTTGAATTTAAATCTCAGAAATAATCTCCTAAATTATGATTAGTTACGGCAATATCAGAAGTTATTTTTCCTTTAAATAGCAAGTCTTTTCTAATTTTTAAAACTTGTTCTTTTGTGAGAGTATCAACAGCTTTAGCACTTTTTAGAAAGTCCAAATTGTTTAAATCAACTTCATATGCATCTTTAATGTCAAATGATAAGTCTAAGTCTTTTTTATAGTACTTTACAGATTTTTCTATATTGCTTAAAATGTCGTTAAAAAATTTATAATTAAGATTAAGAAGACTTCCTTGAGATATATAATTCTCTAAAATATATTTTATTGTAGGTTCAGGATTCCCATTTATTGTAGCAGTTAATCCTGTATTTGATTGTACAAAACCTATATTAACGAAATTTTTGTAAGCATTTAAATAACTTTCAAATCTTCCCTCATGTAATCCAAAAGCATTAAAAATTTTTTCATTTAATTCTAATAATTCTTTTTTAAGTTTTAAAGCTTTCTTTACTAACAAGTCAACTTTGGAACTTGTTGTATCTTTTACAGTTTCTACATCACTAACAAAATATTTTACCTTTGATAAATATAAAGCTTTTTCAACTTCATTTGCTTCTTTTTCTTCTTTTGTATTTTCTTTTAATCCATATTTATAGATGCTAGATGGTTTATTATCTCCAGGTTTTGGGGCTGGGCCTGGACCTGGTTCTGGTGTTACAGGTGTAACTGTCGTACTTGGTGTTGAAGGTATATTGCCAGTAACATCATCTCAAAAACATGATGTTGTTATAGTTGGTAGACTAACAATAGCAGTTAGAGTACCTAGTCAATTAAATATTTTATTTTTTTTCATATTTATCTCTTTCTACAATTAAATAATAACAAAATTTTAATTAAATATCTTAAAAGTGAACAATAAAAATGCAACCATAAGGCTACAAATTTTATTAAACATATTCATTAGCTTTAAAATAAGGAAGACCTAAGTTAGTTCTTAATTCATATAAATCATGATAACTATCATATAGTTCTCTGGTTATTTCTATCTTTTTATTATTTTTATTAATATATGTTTCATATTTAATATCTTTTCATTCTTTTAATAAATAGTTATTATTAATTGGACTAATTTCTTTTGAATTTACATTGTATTTTTCTTTATATTCATTATAAGAAGGATCATCTCAAGTACAATCTACAAAATATTCTTTACCATTTAATAAAACTAGGTTTCAAGCATGGCCCTCATCATCTTTTAGTTTGCCATCAACATAAGTTTTAACTGGTCCAGTTATATATCAAGATGGAATATCTAAAAGAGTCATAAATCTTTGAAATGTTCTAGCATAAGCGTCACAAACTATTCTTCAATTTCCAATATAAGCATATGGACTACGACAAGTTTGAGCTCATTGTTTGTCACAATATACCATCTCATCATCTCTTAAGTAGTCATTATTTAAAATGATTCAATTGTGAATAGCTCTAATTTTTTGTTCATCATTCATTTGTTTTGAAATAATAGAATAAATAATTTTTTCAAAATATTTATTGATATCAATTTCTTCTTGTGCATTTTCAGTTCTATAAGCTAAACGATTTATTTTAATGTTTATTACATTATTTTCTGGTTGATAAAATGTAACAGATGTTGAATTTTTATAGTGGTTTCCTTTAAAACAAAAATCATTAAAATAGTTTGAACCGCCCGTTAGATCATAAACTAAATTTCTTAAGCTGTTATATTTATCCGTGCCATAATAATATTTGATAAGAGGAGCAACATTAACACCATTTTTAACTAATGATTTTCTAAAATGTTCAACATTCAATTGTTTGTTTAAAACTGAGTTAATATTTTCTGTATACATTTTTAAATTATTAATATTAATTTGTTTAGCATCTTTTAAATCAAAAACTAATTCAAGATCCTTTTTGTTTCTATCAATAAAGTTTCTAAACAAGTCATAATTTTTGTAGAATAAATCTTTATTATTGACTATTCGATCAAAAGCACCTCTTTTGTATTTGTCTAAATAAAATGAAATTCAGGAACAAAAATTATTATGTTTTGGAGCTAAAAGATCAAAAATATAAGCTCTGCTATTTTCATTATCAATATATAAATTTATAAAATTTTTGTAAGCAACTAAATATTTTTTATAACGGTTTTCATGCAAACCAAATTTATCATAAACATCATTGTTTAATTGCAATAATTCTTGTTTAATTGCTCAAGCATCTTTAATAATTTTATTAACTTCAGGATCTGAGCTAGCTTCAAAATTAGTTATCTCAATATCTGAAACAAATTTCTTTATTTTTAATAAAGATAATTCTTTTTCTTTAGAATTGATTTCAATTTCTTGAGCAGTATTTGGTTTTAAATAATATTTGTAAATACTGCCATCATCTTCTCATTCACCTTCAGGCACAAAATTAGGAACACTTAAACTTGGCTGTTTATAATCTTCTCAGTTGTCATCATCGTTTGAAGGTTTTTGTGGTTCAGCTGAATTATTGCAGCTTCAAGAAGAAAATGATGACATAAGTAATAAACATATAATTGGACCAAATGACATTAATATTTTTTTCTTCATCAATCCTCCTATTTTAATTATATTAAAATTTATAAATAATATTTAAAATTCAAGACTTTGAAATCAACAAAAATAAACTAAAACGCCTGTTTTAAAGGCGTTTTGGAAAATTAAGCTTCATATTTTTCTGGGTGTTCTTTCATCATTTTCTTGATGAATTCTTTTTTAGGCATCTTGCCATATTTAACCATTAATTCTTCACATTCTTTTAATTCTGCTAAGGCTCATTTTTCGCCTTCAAATCCTTGAACTTTTGTAGCTCCTTTTTTCTTTCAATTTTTATAGTTGCTAAAGAATGTTTCAATGTGCATTAAAAATGGTTCAGGTAAGTCTTCTAATTTTTTAATACCATCTAAACGATAATCATCAGCATGAACAGCAATTAATTTTGTATCTGTTTCTCCTGAGTCAATCATTTTCATAGCACCAATAATTCTTGCATTAAGAACAACACCTGGTGTAAATGTTTCTGGAGAGTAAAGTAAAACATCTAACTCATCGCCATCTCAATCTAAAGCTGTAGGAATAAAGCCATAATTACATGGGTAGACAAAATCTCCTCTCAAAATACGGTCTACATGAATATTTCCGTCAGCACGATCATATTCGTATTTTATGTTTGAATTCTTTTGAATTTCAACTTTCATTTCAATAATATTACTCATGCTATTATTATAGTTAAATAAATTAAAAAATATAATAAAAAGCAGCCTAAGCTACTTTATAATGTCGAAATAGTTTTGCCAACAATCATTGTTATACCAACAACAACAAATAAGATCCCGAAAAATAATGGTGCAAATAATTTAATTCTTTCTCAAGCTGGTAAATACATTCCTGTTGCTTCATAATTCGTTACTTTAGATTTTGGATTATTTTTTTTATATTCTTCCATTTGTTTTTCTTTATACAAATAAACCTTGTCTTTTGCTTTAGGATAAAAGAAAAAGCATGTAATTCCAATTACTATTAGTGATAATCCAATAATAAATAAAGCTAAATAAGGAGCATTTAATTTGTGTGTTCCTTGTTCGTAAAATGGGTTATTTGCTTTATAAAATACTGAGTTTAAAATCATTTAAGTCCTTTTTTAATGTTAAAAGTATAACAAAAATAAAAATATTTTGTACATTCTTTTATTTGTGTTTTTAATAATCAAAATTTAATATCTTTTATATAATAAAAGCATTATGACTAAAGAATTAAAAAAACCTAAAATAATTTTTATCGATTTGGATGGTACAACAATTGATAAAAGTAGCAAATTTAAAAAAGATATCAGTGAAATTAACCAAAAAGGTTTAGCTAAACTTAAAGAAAAATATGGCTTAGAAATTTTTGTTTCAACTGGCCGCGCAGTAGTTGAAAAAACAAAAATGTTGGCTAGAAAATGTGGCAATGAAAATAATTTTGTTGCTTGAAATGGGGCTAAAGTTTTAAAAGATGGTCAAGAAATTTTTTCAGCAGCTATAGATCAAGAAGTAGTAGAACAAATTTTTGAATTAGCTGAAAAATGAAAAATGACAATTATTATTAACTCAAACTTTAAAAATGGTTTGTACTCGAATTCATTTCTTTTTAAAGTTGCTTCAATTTTCAAAAAAGGCAAATGCTACAAACTAAAAGAATTTAATAAAGAAACTGAAGTATTTAAGTTACTTTTTCTTAATTTGTCAAAGAAAAAACTTCATTTATTTGCTGAAGAACTTAAAAAATTATGACCAAAAGATCTTAATGTATCTATATCAGGATTTAAAAATGAATTTTTAGAAGTTACATCATTTTTTGCTTCTAAGGGTAGTTCAAATAAATACTTAGCTAAAAGTTTTGGAGTAGATGCTGAAGATTGCTGACATGTTGGTGATTCACAAAATGATTCAACAGCTAGTGGACATATGGGTTATGTAATTGCTATGAAAAACTCTGTTCCTGAATTTAAAGATAAAGCAGATTTTATTAGTCCTTTTACAAATAAAAAAGGTGGTTTATATAAAACTTTAGAAATGTTACTTACAAAGTTTGAATAATTAAATTTAATATTAATTTATAATTTAATTTATGAAAGCATTTTTTAATTTACAACTAAAAATATATTTTAGACACGCATCAAGCTATGTCGTGCCTTTAATTTTCGGTATCTTTTTTATCATTATTAATGTTGTTCTTAAATGAAGTGCTGCAAGTCCAAAAGTTTTTGAAACTCTTTTATATTCGGTGAACTTCTTAACAAATATTAACCATTTTTCAATTTTTATGATTTTTATTGTTGCCACTTTTGTGTGTCAAACTGTATTTTATAAATACAAAAGAGAAGGTGTGGATTACATTTTATATTCAAAACCTTTAAGTAGAACTCAAATATATTTTGCTAATATTTTTGTATCTGCAATTGGTCTATTATTTTCAATAGCAATTATCGAATTAGGTTATTTTATAAGCAGATTGGTTTATCCAACAATTATTTGAAAAGAGGTCTGAGGTGCTTCAGGTGCTCACTTATTAGCAATAACACTAACAGCTCTTTTTGCTCTAGGAATTGCTAGCTTAGTACACTCAAGTGTGGGTTTAAGGATGTTTCAAATTTTAATAGGTGCTATACCTTTTCTTATAACAATTATTTTCGGTGTTGTTAAGTTTGCTTGATCTGTAGATAACATTAAAAGCTTAACTAAATCAACAAATAATGTTATAGCGACCTTGCCTTCTAAAGAAAAAGGCAACAAGAAATTCCTTGAAGAATTAAAAGGCAGATTCAATGAAAATCAACCTATCTTGATGAGTAGATACAACGATGACTTAAAAAATACTTTATTAAATATTACAGAAGATTCAGAAGATATCACAAAATATATTGACAATACTCATAAGTCAGTTTATAACTATTTATATTGATTAAACATTGAAAACTATTTCTCATTACAAAGCGGTTGCTTTGATCATCGTATTAATTCATTTAACAAAAAAATTAGTTATTACAAAATAAATCCACTTAAAAGTGACGGTACTATAGATTATCGAATGTATGAAAAATATGGCTATCATTTAGACAACAATATTAAATTAAATATCTGAAATGTTGTTTATCAAGATAACAATAAAAATTCTATAAAAGAAGTTAAATCAAAAAATCTTTTTGGTGTTAGTTATGATGCAAATTCAATTAAAAATAAATTAAGTAAAGGCTACTTAACTAAAGAAGATTTATATGAAAGTTTTTATAAAACTTATAAAGAAGAAACAATTGAACCAATGATTGATAAGTTAAAACTTTTTGAATCAATATTTTTAGATAAATTATATGAAAATATTCAAGAGATCAAATTTAACAAATTACCTTATGTTATAGATTACGAGGGCAAAAAGGTATTAGTCTTATCAAAAGTATTTCAAGACTTTAACAAAATATTAAGCTATGTTGAAGAACATCAAAAAGATAAATACAAAAACTTAAAAATTAAAACGTATATTGCAACATATTATCCACAATGAATCGATTTCTTTGAACAATTTAATGATGAAAAAGTAGCAAAACTTAATGAATTTTTTGTTGCTGTTGCAGAGCTACTCACAATACAACCTAAAAAACCAAAATCTAATTCAATTGGTGAAAGAATTAGATATGAAAAGAAACTTAAAATATATCAAGAAAAATTAGAAGAAAACAAAAAACTTTTTAAAAAAGTTGGTTACTTAATGACTCTTATGTATGGTTTAAATAAAGAACAATATGTTTTAAGTTATCAAGCTTCACCTAATAATTTGGATGAATTAAATGAATTAGACAATGCAGGTGGTATTGAATTAATTAAATTAACACCACTTGAAAGAGATTTAATTGTTGTTACAAGAAAAGATCACATGACAGTTGGCGGATCAATAATTTTATCAATGTTAGCTGCTTTTGCTATGATGGCTGGTGGTTGAGCAATATTTGTTAGAAAGAATTTTAAATAATGAAAACACTAGTTAAAAAAAGTAAATGAAGAATTTATCTTGAAAAAAGAGCAAATTATAAAGCTACAATCAAGAGAGCTAAAAATTATGAATATCAACAGCCTCAAGAGGATGTTGCAATTTCAATTCAACATTTACATAAATTTTTTAAACTTGGACATGGCGATTTTAAAACTGCTTTAGACGATGTTTGTTTTGATGTTAAAAAAGGATCATTTCATGGTTTTATTGGTGATAATGGTGCCGGTAAAACCACAACTATAAGATCAATACTGGGATTCTATCCTTCTAAAATAGGCAAAATATTTATCAATGGTATTGATTCAACAAACAAGAAAGCAAAATCATTTATTGGTTATATTCCTGAAGTTTCTCTTTTTCCTAGAAAAGTAAAAGTAAAAGAATATCTTTTAGCATTTGCTGAAATGTCAAATTTAAAACCTGAAGAAGCAAAAGAAAAAGTAAATTTCTTATTAAAAAAATATCACTTTGATGGACCTGAATTTGAAAAATCAGCAGAGCAACTTTCATCAGGCCAAAAGAAAACAATTTTATTAATGCAAGCTTTATTAAATGATCCTGAAATTTTAATTATGGATGAACCTGCTGCTAATTTAGACCCAAGTGCTAGAGTGATATTTTATGAACAAATAAAAGAATTAAGTTTACAAGGAAAAACTATATTTATTTCTAGTCATATTCTTCTAGAACTTGAAAGATATATCGATTCTTTTACGGTGCTTGCTAAGGGCAAAGTACTAGACAATGGAACAATAAAAGAAAAATTGCACAATGCAAAATATAACAAAAAAATAAATACAAATGATAACAATAAAATAGTGGAATATTTAATTAAAAACCGAATTGATTTTATTGAAAAACATGATTGAATTAATGCAAAATTAGATGAAGAACAAGTTAATAAATTTCTTCAATATTGTTTAAAAAACAATATTCAAATTTATAATTTAGCTGATAACAAATTAAGTCTAAATGAACTTTATTTTAATGCCGAAAATGTTGAATAAATAATGAATAAAAATAGAACTGAGAGATTAAAACTCAGTTCTATTTTTTTACGAAAACTATTGTTTTATAGGATTATTAGGATTTTGTGGTTGTCTTTGAGCTACATTTATGTTTGTATAATTTTGGTTTGGATAATATGGATTTTGTCTAGGAGGATATTGTGCAAATCTTGGATCAACATAGCCTTTATTTGGAAAGTTTTGATATTGGTTTGGATATTGAACATTTTGATACATATTTGGATTTACTTGGCGAAGTCTAGAAGTTTGTTGGTATTGATCTTGAGGTTTATTTAAATTGAATTTAGGTTGATTTGGTTGAGCTTGTTGTGCAGAAGGATTTTTAACTTCTTCAACTTTAATATTTTGTTCTTCAACTTTAGTTTCAGGAACAACTTCTTCCTTAATTTCTGTAATTGTTTCTTTTGCATCTTCTTCTATCTTTTGAGTTTCAACCTTTTGATTTTTCTTTTCTTCAATTTCAGCTTCTTCAAAGTTAGCCAAGTTTTCTAAATCAACAACTTTTTGAGCTTCTGCTTGTAATTGTTCAAGATTATCAACATTTTGAACTTCGACATTTAAGTCAACTTCTTTTTCGCCACCATTTTCATCATCATCTGAATTATCTAATTTGATTTCACTTGAAAGTTGATGACTATCATTCATGTAATATTGACAGTCTAAACCTAAGTATGATTCTTTGAATAATTGCTCAATTAATTCAAGATGTTCAATTTGATATTTCTCAGTAATTTCTTCTCTTGCTTTTTCAAAATTAATGATATTTTTATTAGATTCAACTTTGTTGTATTCATCTTGTGTGAAGTCCATTAAGATTTCTCTATTTATTACAACTTCTGAATTTTCTTTATTAGTTAATGTATCAACAATAGCATTTGCTACAAATTCTCTTGATCAGTAGAACGATTCAGTTAAATTCATATCTAAGTTTAATTGTGAACATAAATTAAAGGTTAATAAATATGCAAGTGATTTAACTGTAATTGCTTTACCATCTAATTTAATGTTGGCAAATACTTTTTCTTTGCTTTCATTTTCCACTATTTTAAATAATCTATTGTATTGTTGTTTTTTAATTGAATTAAGTTTGTATAATCAACCTTTTTCTTCTAATAGGTTGCTGTATTCATGTGAGAATAAGTTTTTGTAAATTAAGAATGCAAATAATTTACTTCTATTGTTTTTCAATGCTTTACGAACATAAGTAATTAAATATCTACTTTCATTAGGTTTTTTTGTTTTAAAGAATATTTCAAGATCTGAATTATTTCTTAATAAATCTAATTGACTTTCAATGTAAGGATCAAAACCAATTTCTTGATAGTGAATTGAACAACCTGCATAAGCTAATTCTTGAGCTATCTTGTTGTTTTCACCTAATGTTTGTTTTAAAGCTTTGAATGCATTTAAAGCTAAATTGTTTTGATTTTTGATTTTGCTAAATACATTATTTTGCAATAAACTGTTTACATATTTAACAATTTCTTCAAATTTATTGTTGTAATCTTTTTTGAATTCTTCTGCAATATCATCACCCATTGTGTTAGTGCTTCTGAAGTTATGGATATCTGAGTACATATTAAAATCATTAATTAATACTTTGTTTAATTTAACGTCTTCAATTTGTTTTATTCAGCTTAATTCTTTAACACTTAAATTTGATTGTGATAACAATTTATTGATTTGTTCTTTTTTGTATTCTTTGCTAATTGGTAAAACAGTTAATGAAAAATCAAAAATATTACCTTTGTCTTCTAGTGATGCAAGTGTGTTGTCAATTACATAGAAGAATTTAATTGCTTTACATTTTTTAACATAGTATTTTGATGCATAAAATTTAGCAATTTGTTCTTTTTTATTTTCAAGTTTAGCTAAAATTTTTTCAATTCTTTTTTGTCTTCTTTTGTTTACAAGTGTGTTAATATCTTTAATTCTTTTGATTAATTGATAATCTTTAGATTCGCCTAAATCTTCTATAATCAAAGCATCTAAGCCAGATTTGTCAATTAAGTTAACAAGTTTTTCTTTATCTTCAATAACTACTATTTCTTTAGTTTCTTGAACTACTATATTTTTTGATTTTTTTAAGTCTAATAAATAGTTTCTACCATAATAAATAGCAATTGCTAAGAATCCAACTATCATTCCAAATAAAATAAATGCAACTGGTAAGAAGAATCCTAAATGGTTTAAATCAAATGATTTGCTTCAACCATGAGCAATAATTTTTCTTGCACTAAAGCTAAAAAATACTACTGATAATAAAATGAAAATACTTGTTAAAGCTGTTCATAATACAACATGTTTTACACTTGTTTTATTATTTGCTTGATTAACATTCTTTTTAGTAACTTGGCCTGAAATTTGATTAATGATTTCTGATTCTAATAATTCATTATTTGTGTTTACTAATGAAACATTAGCTATATTAAAATCTTTTCTACTTGATTTATATGTTTCAATAACATTTTGATTTTGTCCATCTTGAGAACCTATAATAGCAATATTATTCACTTTGTCATTGTTGTTACTCATTGCAAAATCAAGTGATTTTTTATATAACTTGAAAGCATTAGAATTTTTCATGTCTTCAGTTGAATAAAGCTCTATAGTTTCAATAATATGTTCTTCATTTTGAACTTTATTTTGGATGTTTCCATTTCTAATACATTGGTGATTTGTAAAAGAAGAAGAATTATTATTTTGTGACATCCCCACCTCCTTATATTAAGTAAAAAAATTATACCTTATTAATTGTATTAATAATTAAATTGAAAAATAATTTATATGCTTTTTTAAACAAAAATTATTCTAGTTTGTCCAAAAATTATAAATTTTTGACAAATAATACTATTTGATAAGACTATTGGCAGAATGTAAAATTTCAAATAATTTTTTTATACTTTTTAATAATAGTTTGCTTATTCTTTGGTATTATATTTACATAACTATTAATAGTAAACTGTATTGTATTTCTACATCTTTATAATAATCCTTCATAATAGAAGCATAAAGGTACTTTTAGCAGTTTTTTTAAACATTAAAAAAGGAGATCAAAACATGAATCCTACAAGATTAATACCTATTGGTGGAGTTCAAGAAATTGGTAAAGCTGCACTAATAGTTGAATACAAAAACCAAATATTTATTATTGATGCAGGTATCAAATTTGCTGATACAGCTTTAACTGGTATTAAAGGTATTATTCCTGACTATACATATTTGGCAGAAAGATATAAAAATATTGAAGGTCTTTTTATTACTCATGGTCACGAAGACCACATTGGTGGTGTTATTCATTTAATGAAACAAGTAGCAGTTAAAAAGATTTTTGCACCTAGAATTGCTATTCAATATTTGAAATTAAAATTCGATGAAAATAAAATTAAAAACAAGGTTGAATTTATTGAAATTGAAAAAAGTGCTGTACACACTTTTGGCGAATGTTCAGTAGATTTTTGAACAGCTCAACACTCAATCCCAGATGCTTTTGGTATTAGAATCAAAACTCCAAATGGTTCAATAATGTGTACCGGTGACTTTAGATTTGATTACAACCCTATTGGTGAAATTTATACTGATTTTGCTCGTTTAGATCAAATTGGAAAAGAAGGCCTAACTGTTCTTTTGTCAGACTCAACTAATGCTATGCGTCCTTTCCACTCGCCTAGTGAAAATGACATCTTAACAGATATTGAAAAATACATGCGTCAAGCTACAAGAAAAACTATTATTACAGCTTTTGCTTCAAACTTAACTAGAGTTACAGCAATTATTGAGTTAGCTGTTAAACTTGGCAAAAAAGTTGCAGCTTTTGGTCGTTCAATGGTTCAAGGAATCAAAATTGGACGTAAATTAGGTTACATTAAAGCGCCTGCAAGTGTTTTTGTTGATAAAAAAGATGTATCAAAAGTTCCTGACAAAGATCTTGTTATTCTTACAACAGGTTCACAAGGTGAACAATTGGCTGCTCTTTCAAGAATGTCTTATGGTAAACACCAAAGCGTAAAAATTGAAAAAGGTGACATGATTATCTTTTCATCAAGTCCAATTCCAGGGAATAGAATGGTTATTGAACTTTTAGTTAATAGACTTTCAAAACTTGGAGCTATTATTAAAGAAAATGGTGTTGATGGTTACTTGCATACCTCAGGTCATGCTTACAAACACGAACATGATAAAATTTTCCAACTTACTAAACCAAAATACTTTTTACCTTACCATGGTGAATTCAGAATGAGTATAGTTCATGGCCAAAGCGCTATTAGAAATGGAGTTAAACCAGAAAATATAATGATTCCTGAAATTGGTCGTGTTTACAACATGATTAATGAAGAAATTACACAAACAGATGAAAAAATAGATTGTGGTCCTATTTATATTGATGGACACACAACCTTGAATTTAAGTGCAAATATACTTAAGGAAAGAGCTAAATTAGCTGATAGCGGTTTTGTAAATATGATCCTAAATATTAACAAAAAAACTAATTCAATAGTTGGCCGTCCAAGTTTAATTAGTCGTGGGAGCTTCTATGTAAAAACTTCGCTTGAATTAGTTGAAGAAGCTAAAAGAATTGCTCATGGTGCTGCTCTTTTCTATATTAAAAATACACCTGATTGAAATATTGTAGATTTAAAACAATTGATTATTGATCGTCTTGAAAATCTTTTCTACAAAGAAAAAAGAAGAAGACCAATTGTTGTGGCAACTATTCTTTTTACAGATGAAGAAAATGAACCAGCATTTGCAACTTCTAAAATTAAATTTAATGTTAAGGGTACAAAAGAAGATGAGAAAAGCAAAAAAGCTACTGCTGCTCTTAAAGAAGCAAAATCAGAAATGTTTGGTGAAAACAGTGAAATGTCAGAAGACATTCATGATGAAGAAGACGAAGAATAATGATAAAAAAATTAGCAATTTGATTGTTGCTAATTTTTTAATAACACTATAAAACAGTGCTTTTGGTCAAATAAATGATTTGGTACACCGTGCAGGGTTCGAACCTGTGACCCGCTGGTTAAGAGCCAGCTGCTCTACCGACTGAGCTAACGGTGCTTAAGTGCATTTATTATATATCAATTGTTTTTTTCTCAAAATATTTTTACAAAAACAATGAGTAAAAAGTGCAATTTCAATAGAAATGAAAACAAATAAATATAAGATTTTTTGAATGGTGGATCTCACTTTTATAACATTTGTTGCTATTGAATAACTTAACATTTTTTGTCATACTATATAATTAGTTGCTTAAAGTAAGTATTCAAGAACAGTGCATTCATTTCATGACAGTTGTCAAATACTCTTTTGGTGACTTTTTTATAAAAAAGTTGAATAAAAAAATGCAATAATGCACTTTTTAAACTTCAATTGCTTCATTTTCTTCATCAACTAATTCATTAACAATAGAATCTCTTTTAGCAAGAATAATTCCTGCTACTACATAGAAAACAGGGACAAAGAAAATTCCTAAAATTGCTAAAACATAAATGTAACGATCTTTTTTAAGAAAACTTAATTTTGCTGCTTGAATTGAACCAACAACAATAAAAGAAATGCATAGAGCAAGAGTAATAACTAATAAAATAATGTTAGTAATTCCAGCCACATAATTGTTATTTGTCGCTTTTATTACTGCTTCTGGGATATTTTTTTGTAATCCAAAAAATGAGATAGTTAGTGTTAATAATAAAATTGCTGCTATAGCAATTGAAGTTAAAGTTGATTTAAAACTTTTTTTATTCATTTAGTTTCTCCTTATTATTTACAATAAAATTATACAAAATATTATTTCATTTCTAAAAGGTTAAATATGCAAAATTAATTGTTTTCATTTTTTAAACTTTTGTATATAATAAAAAAGCAATTATAAATAATTGCCTCTAACTAGTGGGAGAGAAAACAAAAAGTATCATTAGTACCACACTTATCGAAAGGATATTACAATGGCAAAATCAAAAGCTGATATTGTCCGTGTTCGTAAGCTACAATTCATCGCTGGCCAAGCCAAACCTGGTCCAGCTTTAGCTGGTGTTGGAATCAATATGCCTGATTTCACACGTGCTTTTAATGACGCAACACGTGATAGAGGTAATGAACCAGTTCCTGTTGAAATTACCGTTTACAAAGATAAATCATTTGACTACAAGTTATTTACAGCTCCAGCTTCGTACAAATTAAAGCAAGCTGCTAAATTAAACAAAGGTAGTTCAAACTCAAAAACAACAATTGCTGCTACTATTACAAAAGAACAATTGAGAGAAATTGCTGAATACAAATTACCAGACTTAAACACAAATGATGTTGAAGCTGCTATGAGAACTATTGCTGGAACAGCAAGACAAATGGGTATTATTATCGAAGGATTTGATAACGTTGCTGCTAAAAAAGCTGCTGCTGCTAAAGCTGCAAAAGACAACGCAGCTGCTGCAGCTAAAGAAGCCGCTTTAGATGAAGCTCAAAACGAACTAAAAGAATCTAAAGGTAAAACCATTGATGTTAAAACAATTTCAGACGAAAAAGCTGCTGCTAAAGAAGGAGAAGAATAATGTCTCTTAAAGGTGGAAAGAAAATAAGAAATGCTCGTGAATCATTTGACAAAACAGTTGCATATGATTTAAAAGAAGCATTAGAAATTGTTAAAAGAACATCATTTACAAAATTTGTTCCTACAGTAGAATTAGTATTCAAATTAAACCTTGACGTTAGAAAAGCTGACCAACAATTGCGTGGATCAGTATTGTTGCCTAACGGTACAGGTAAAAGCGTTCGTGTATTAGTTGTTTCAAACAATCCAGCAATTCAAAAATCATCAAAAGCTGCTGGTGCAGACATCGTTGTTGATGGTCCAGCACTTGAACAAAAAATTAAAGAAGACGAATTTGACTTTGATGTTATGGTTGCTGACCCTTCAATGATGCCTTTATTAGGTAAATATGGTAAAAAACTTGGACCTAAAGGTTTAATGCCAAACCCTAAAACAGGTACAGTTACACCTACTCCTGATAAAACAGTTGCTGAACTTAAAAAAGGTAAAGCTAACTACCGTACAGATAAAGCAGGTATTGTACATACAATCATTGGTAAATTAAATATGACAACAGACCAATTAGCAGAAAATGCTCAAACAATCATTAATTTAATTAAAAAACTTAAACCAGCTACAGTTAAAGGTGCTTACATGCAAAACTTAGTTGTAACATCAACAATGGGTCCTGGTGTTAAAGTTAAAATTGAAAAATAATCCAAACAATTAAACTTCCTATTTAGGAAGTTTTTTGTATAAAAAAATAAGCCAAACTTTGACTTATTTTATTGTTTTATAGTGCTTTTAATCAATTTTAATCTTTAAAGTTTCCTTAATTACTTTAACAAGTTTTTTAGCTTTTTCCATGTCTATAGTATTAGGAATTCCAATTCTGACTTGTTTACTTCTTCTATTTCCACGACCTAATTCAAAATAAATATTGTGATTATATTCAATTTTATTTCTTAACTTAATTGCATCAACACCTTCTGGAACATTTAAAACTAAAAGTCCAATAGTTATATTCTTTTTAGGAACTACATCCTTAAATCCAATAGCAATTAATTCTTTTTTAATGTAGTCATATATTTTGAGTTTATTATTTCTTATATTCTTAAATGTTTGGTTTTTGATAACTTTCATTGCAGCATTCAAAGCTAATAAAATACCTGAAGCTGGAGTATAAGGAGTTGATTTTTCTCTTTCATAAAAATTAATATAAGCTGATAAATCAAAATAGAATTTAAAGTTTTTATTTGCTTTAACTACTTGTTGAGCTCTTTTAGATAAAACAACACAACTTAAACCAGGCATTACACAAAATGCTTTTCCGCTTGTTGCTATAGCAACATCTAAACCTCAATTATCAAACTCAACATTTTCATGAATAAATGAACTAACTGTATCCACAATAAATAATGTATCTTTAGCATATTTTTTAACTACTTTTGAAATAGCTTCAATATCATTTAAAACACCTGTTGATGTTTCATGATGAGTACAGAAGAATGCATCATATTTATTTTCTTTAAGTTTATTTTCTATATCTTTTACTTTGAATGCTTCACCATCTTCATAATGAATAACATCAAGATTATTAGCTCCAATTAATCTTTCTAAAATTATTTGGAATCTTTTACCAAAGTCACCTGCTTCAACCAATAAAACTTTGTTTTCAGGACTAATAAGGTTAACAACAACTGCTTCCATAGCCCCAGTTGCTGAACATGTAATAAATAATGGAAGTGCTTCTTTAGTTCTAAAGAATTCTTTAATTAAATTAGTTGATTCTTCCATGTAGTGTTTGAACAACTCACTTCTATGATGCAACACTGACTCAGATAAAATCTCAGAAACTTCTGGATAATTATTAATAGGACCTGGTGTAAATAATAAAGTAGAAGCGTGGTTTGCATAATGATGATCAATATTAATTAAGTCTTGAAAATTGTCAAGTTCTTTAATCAAATTTGAAGAAATTGCTTCCAATCTCATTTTGTTTTCTAAAGTTCATAAAAATGTTTCAAAATATTGTTGCTTATTTCCTTGTTGATTAAATCATGCATCAACTTTTTCTTTTGCTTCATTAGCTAATTTTTTATTTAAATATAAAATACCAATTCAGATATTTTGATTATATGCATCTTTTTCATTGTGATGTTTAACTACATATCCTTTATTATTTGTATCAAAAACTCATTCCGATTTGTGTTTTGTACATTTTTCAGTAAAACAAATTGAATCTTCAGAGTTTTCAATTATTTTAATTATTTCTGGAAAAATATTTTTTTCAATATACAAATCACCTTCAATATATAAAGTGTCTCCAAATGCATTTTTGGAAACAACATAAGAATAAATGTTGTTTGCTTCATCATATAAATCATTTTCAATTAATTTGACATTATATTTTTCTTTAATTAAATTAAATTGGTCTTTCATATAACCAACAACCACAGTGATGTCATTAATGCCTATTTCTTGTAAATGCTTTATGTTTCTTTCAATCATTGATTCATCATTTACAGTTATTAATGGTTTAGGAACATAATGAGTTAATGGGGTCATTCTAGTCCCCTTACCTGCTGCAAAAATAACAGCATTTCTAACTTTTGCCATAATTACCTCCAAACATTTATTAAATTATATATTTAAAATAAAAATTGATTTATATAAATATAACAAACAACAAAAGAAAAGTTAATCCTCCTAAAGCAACACTATCAAAACGATCTAAAATACCACCATGACTTATTAAAATTTTTGAATAATCTTTTATCGCATTTATTCTTTTAACATATGAAAAGTATAAATCACCTAATATCGAAAAGAAAGGCATTAAAACAGCAATTGATATTTGTGCTTCTAATTTTCCATAGAATAAATTAAGCCCTAATATTAATGCTAATCCAATTCCAATTCCACCAATTAAAGCAAAAATAAAACCTTCTCAAGTTTTTTTAGGTGAAACATTTGGAGCAAATGATTGTTTGATTCATTTTTTGCCTGCTAATGTTCCTCCAACAAAACCAAAGATATCACAAGTAGAAGGAATAACAAATAATAGGATGGTATATCTTCAATCAAGTATAATGGTTATTTGAATCAATTTTGAAGTGTTGACTATTACGTATAGAGAAATAAACATTAGTATTCCTCTAACCAAACGATCTTCAAAACTCATATTATTTCTTGTTATATATTCAATAATAATAAAAGCAACAGAAATCATTAATAAAACAACTGGTGTAAATCATTCTTTTGCTATTTCTTGAATAAAACTAGGATGAAATTCTAAAGGATGAGAAACAAAAATAAACTCAGTAAAGTTATTAACCGGTAAAACTACAGTAGGTAGTATAAGCATAATAAAAATAGCAATAACATATCATTTTAAATGATTAGCTTTGAAATATTCATAGCATAGTATTCCTATCCCAATAGCAGTAAAAATATATGAAACTATTCTAGCTTCATAATGATTTTTTCCAAAAATTGCTAAAGGTACCATTCAACTTAGCAATACTACTACGAAAATAAAAGCAGGTAGTATTCTTGATTTAAAAAATTTCATGTTAATAATTATACATTTTTTTAAAATAAAAAAATATTAAAAGTTTGTATGAAATATTCCTTTTTTATAGTCATTTTGGTCAAAAAATATCCTTTGCATTAAGCAAAGAATATCTAAATTATTATTTTTCTTTAAAAAGTTCAATATCATCAGGGTGAATTGGTTTTTGATTAACTTCAACTTTTGCAATTCTTCCTTTAACAACATGAATAATTCTATCAGCCATTTGCGCCATAACTGGGTTATGACTAACCATAACAACTGTAGCGCCTGTTTCTTTATTAATATTGTAAAGAAATCTTAAAACTATTTTTGTTGTTGCTTCATCAAGAGCACCAGTAGGTTCATCAGCAAAAATGATATCTGCATTTTTAGCTAAGGCTCTCATAATTGAAACACGTTGTTGTTGACCACCAGACATTTGAGCAGGGAATTTTGATATTTCTTCATCCATTTCAAATTTCTTAAATAACTCAACAACATCAACTTTTTTAGCAGGATTTTTTTGTAAGTAAGCTCCCGTTTGAACATTGTCAAAAGCATTTAAGTTTGCTAACAAGTTATAGTTTTGGAAAATGAAACTAACATGTTTTCTTCTAAATAGAGTTAATTGACTATCAGACATATAAGGAAGATTATTATCGCAAGCAATAACTTGTCCTTTTGAAGGTCTATCAAGTCCTGAAATAAGGTTTAGAAGTGTACTTTTACCACCACCACTAACACCATAAATAAGCATTAGCTCACCTTTTTTAATTTCAATTGAAACATCTTTTAAAACTCTTGTAACAATATTACCATTAACATAATATTTGCTAACGTTCTTAACTTCAATAACATTACCTTCTTCATTTTTTAGGTTTTCATTGCCATCTTTTTTTCTTTTTTTCTTAGCTAACTTAGTCAATAATTTTCGAACTTTTTTAGGAACAATAATATTCTTTTTAGAACTATCTCTGTCTAAAAGCTCAAAAACATTGCCTTTAGTAATTTTGATATTTTTGTCAATTTCTAATTCAGCGACTTGTTCTTCAAGAAGTTTTTCTTCTTCAATAATAGCTTGTAAAAGACCAGTTTCTTCTTTTTTATTTTTTTCTCTTTCAGCCTTTTTTGCAGACTTTTTGGTCATTTTTTTGGCTGTTGATTTTTTAGTAGAAGTTTGATCTTTTTTAACTTTTTTTTCTTTGTTTTCTAATTCTTCAGACACACTATTTTCCTTTCAATAAATCTACTGGTTTCATTTTACTAATACTAATTCAGGTAGCAAATGATGTAATAATAAATATTCCAAAAATTACTAAAGCTGTTAAGAATATATGTAGTGGCGTAAGCACTAATGGCAAGGCAATTGACGAGGAGGCCAGCAAGAACTTGTTAAATGCAAATATCATCAGTATTACTATTGGTATCGCCAGAGCGATGGCCAGAACTATAAATGGAATAAAGACACTGAAGAACATACTAATTTTTTCTTTTTGTGTATATCCAAGAATAGATCAAATAGCAATGTTTTTCTCATTTTCACTAATCATAATTGTTGACATAATAATAAGAATAACAAGTGAAATTATGAAACTAATTGCAATAATTGCAATACTAATTGTTCCTATAGTAGAACCAATTTGGGTTGTAAATCCAGCTTCTATATCTTTTGAATCAATACTTGTACCTAAAGCAATATAATGTTTAGAATCATACAATTTAGCATAATTTTCTATTGCGTTTTTAGGCGCATTTTTAATTTGATCATATTTAGCTTTATTAAATGTTAATTCAGTTGAATCTAATAAATTCATAATTTGATCTTTTGTTAGATTCAATCTGGTTTTTAATATTCCAGTATCTGGATTATAAATTTGTTCAAACATTGATTTAAGTGTGTTGTCATCGATACCGCTTAAATCAAAACCATCATATCCCGATCAATAACCACTTAATGAATAAAGCGAAGTTGAATCAAGAACTTGGCTAGGCGCTGGACTATTTGTCATAACACCATTGAAGGCTTTAAATCCAACAACATTTGGAAGTTTGTCTAAGCCAACTAATTTATTAGCTGCATCAACTGTTGTTATTAATTCATTATTAATGTATGTAGGGTTAATACCAACAACTTTAAATGTTACTTTTGGATTTTTTAAGTCAACATTACTTCTTAATGATTTATCTATTTTTGACAACACTTTGTTTGCATAACGTTTTGTATGGTTAAGAACTTCAAATTGGACTAAATCGCCTATATTAATGTTATATTTCTTCGAAGTAACTTCATTTACAACCAAAGGATAAATGTTGTTTTTGACTTTATAGTTATATAAATCATTGTATAAATTATTACCATTTTTATCTACAAGTTTTATGTATTTACTATCTTTTTGATAACCATAAATTTTGAAGTTATTATCTTTGTTTGCAACTTTTACATAAGTATAAGTTTCATCATGATTTGGGTCAATATAAACACCACCAAAACTAATAAAGTAATCTTGAATAGTTGGACCACTTAAATCACTTTTATCAGTTAATCAGTAATCAGATAAATTGGTTGGTTTCTTTTCTTTAGGTTTAATTAAACTTGGATTTTCTTTTTCGGCTTTAATTTTTTGAGCAATTTTAGTATAACCATTGATTAAGAGTGTTCTAAATTCATCACGATATTTATCGGTTGTTATATTTACCATTTGATAATTTGCATTATCTTTATCTCAGTAAGCATATTTGAAACTACCTTGTTTTTCATATGGTGAATGATAGTATTTAAAGAAGCTTAATTTTTCCTTATTAGCCTTATTTACTAAGCTTAAAGCATAAGTATTAGGATCTACAAAGAATTCTTTATCTTTGTCTTCTTGAGTTCTTTCTAACTCATAACCAACACGGTCTCTTATCTTATCAATTTTAGCTTTTTGAGTATCTGGCATACCATTGTAAGCTACTTGTCAAGGGTCAGCGGCTACACCAGCTGCAACAGTAACATTAACTGAGAATTGGCTTAAGATATGTGAGTCTAAATCTTGAGGACTACCATTTTTAGTTCCTTTATTAAGCACACTAGAAGCACCAGGTTTAAAGTAATCTCCTATTTCTCTGTTACCTTCATTTATATCACCAAGAGGAGTATAAATGTTGTTATATAAATTATCAGGATTAAATGGTTTGAATGCTCCACCTTCAACTGTTGGACTTTCTAAATCAACTTTAAATGTATAACTTCTATTTTTATAAGTATCATTAATAGTTCTATTAAAGACGTTTGTATTGGCTACACCAAATAAGGTTGCGATACCTGTTAACATAACACTGACTGTAAATGAAATTAGTTTTCAAAAACTTGTAAATGCCAATGTAAATGAGAAACGTTTTTTAACATTAAATTTCTTAGTTGCTTTTTGATAAGTTTTGAATAATTTTGATTGAGGTATTTCATTAACTCCACTAATTAAGTCTATAGCTTTATGTCTTAAAGCAAATAATGAAACTATAAAGATCAATAAGCTCATTCCAATAAATGGTAAGAAGACAGTGAAGAATAATGTAAAGAAGTTAAATGAAATCGTTTGTTTTGGCAGTGTCCAATAACTACTAAAGATGTTCATAACCATCAGTTGTAGTTTATAACCAATTACATAACCTAAGATTCCACCAATAACTGATGTAACTAAAGCAAACACTGTAAAGCTTAGTGATATTTGTCATGGACGATAACCTTGGGCCACTAAAATACCAATAACTTTGTTTTTATTTGAAATATATCTCTTAATAATAAAGATAGTTGAAATAGCCACAAGTGTAACGAATACTGAAACTAAGGCAATAGTAGCTGATGAAACAGCTTTAATAATTCCTTGAACAGTTGTTATTCTAAGAGACCTTTCAGGGTTAATTGGATCAATTTCATTTGCTAAGAAAACTCTTTGAAGTTTGTTACTGTCTGCAATTGAGTTATCTATTATTTTAGTAATGAATTTTTTAAGTTCATTATTATTCATACCAGTTGCTGCTGAGTTTTTAACTAAAAGAGTTTTCTTAATAACATTTCCAATGTAACCTGAGTGAATTCTGTCAAAACCATATTTGTTAACATAAACTAAGGATTGGTTTTGTGTATTAACTTGTAAGTTATTTTCATCAATAACTGGGTACATATAGTCGACTGTAGTTTCTTGACCAATAATAATAAATTTGGTTCCATTAACATTTAAAATGAATTTTTCATCTAATTTATCTAATAATGATAATAGTTCAATAGGATCACTTGGAATTTTACCTGTATAAATTGCTTTATTATTTTTTGCTAAGTATGCATAGTTAACTTTAGCTAAGTAACTATTTGAATTTGAGAACACAATGTCCTTCATTTCTTTAGGTTCAACTATCGTGTTCATTGCTTCATTAACAAATTTAATAAAATCAATTCCAAATAATGAAGAGCTTGTATCTTTTAATTTATCAAATGTAGCTCCATCCATTACTAATGTTTGTTCGTCATCTAAGTCAAGAGGAATTAGTGAGGTATCTTTTTTGCTAAATGCAGTTCCTAACATGTTAGTTAAGGCATAAAATTTGTCTTCGCCTTTTCTAACAATTTTTTCGAAATCATCTATTTTATTGAGCAATAAGTATTGTAAGTTAGCTTTAACTAAACTAGCATTTGTTTTTTGACTTGCTAAATAAGATAATGTAATTTCAGGGAAAGTAAATCTTAAAATAGGATTGACTAAGGCTTCACTTAAACCAAAAATTTGTAATGGTACATTAAGATTTAATGAAGACATAATGATTTGAGCTTGGTCTTTAATGTCATAAAAATCTTTGCTTAATAAATAATTGTTCAAGAATTTTTGAATTATTCCTAAATAAATACCATCAGGATATTCAGAAGAATTACTGTAAAGTTTGCTTGCTGTAATATAACCTAATTGTTGTAATATTAATAATTTATTATTTTGAATTCATTTTCTATATATTTCATTTTTAGCAGCAAATTGTTCAACTTTGTCATTTAAGAATTTGCCATTAACATCATAAGCAAATAAGTTTGATTTTTCGGGATTTTCAAGTAATTCTAATAATCCATAGTTAGTTACACCAGCAAAAGGTATATTGTCATTTGTTGACATTTTTTTGTTAATGTCAAATATTTCAGGTTCCAATGCTAATGCAATTAAATCTTTAACAAATCTTTTTGCTTCTTCATGATTTGCATATTTATTCATAAACATTTGAATTCATGGATTATAAATTGAAAATGTTTGAGCTCCTAATGCATATTCATTTTGAACAACTGGAGTTGTTAATGAAGATAATAATTTTTTAATTAATGATCAAGTCCCGTTGTTTTCAAAATTTTTTAAATCATTTAATAAATCACTACCTGTTTTATTAGCATCATTACTTATATATGAAGTTCCACCTTTTGTTTGTTTTATGAACCTTAATATTTTATTAATTTTTTCTAATATGACTGTTTTATAGGTTTTTGTGACAAATAAACCATATTGTTTAAGTAATCTTTTTTGAGGTTCTTTTAAGTAATCTAAATTAATTTGAGTGCCTTCCTCTTTAAGACTTTCAATTGATGATTTAAGTGCTAAGAAGTCATGTTCAATTTTGTAGTTATTGAATTCATTAGCTTCTGTATTTGATAAACCTGAAGCAAAAATTTTTAAGAAATCAAAGAAACCTACTTTTGTGTCGTCATTATCTGGAACATAAATTTTTACACTACTATTAGGTAGTTCTATTTCTTTATATTTGTCAGACAAATTGAACATTTTGATTAAGTTTTCTTTAAATGCTCTATTTGATCCAGGCATACCAAACATAGATCTTAAGAATGATATAATTCCATCTTTTGGACTTATTGCATTTAAAACAACTGAAAAGACTTTTTCTTCATCTTTGTTTAAGTCTGGATTAAATTTAACTACTTTGTAATCAATATAATGTGTTGTTATTGTTTGATTTAATTCACGAGCCAGAATATTAAAATCAATATTTGATAGAATATTAATTAAGCCTTGTTTTACATTAGAATAAGGATTTGTTGGATCGCTATTCATTTTTTTAATAATAGGTTTTAAACCACTAATTAATGAGGGATTCATTTTAATTAATAAATTGTAAAGTAAACTATCACTATTATCTAAATTAATTGAAGCAGCTATATCTAAATTATCAACTAATAATTTAAGACCTTCTTTTAATGTTTTTTGGTCTAATGAATTGAATAATCATTTGATTAATAAACCTGATGAAAGTTTTTGAGTGTATGTTTTACCATTATGAACTACTTTTTTATCACTTTCATTTTTATATCATTCATGCGAGTATTCACTAAATTTTTTAAAATCTATTGAATCAATTATTTTTCAAATTGCATTAATAAATATTTTTGGATCATTTGAAACATTAACTAATGATTCTGCACTTAAGTATTGGTTTAAATCAATAGAAGCTAAATCTTGTAGTAATTTGTATAAGTTATTTACTTCAGCTGCTAAATAAGCTTTTTGTTCAACTAAAGTTGATTTAGAAGCTATTTTAGCTTTTGCTTGTTCTAGAATGCTAAAGAATAGTGATTTGAGAACATCACCACCTTCATAATGACTTAACTCATAAACAAAGTCAAACATTAATTTAGGTAATATTTTCTTGTTCATTTTGCCAGTAGCAAACACTGAAGCAAAGTTGTTATCATTTAACACTTTTTTCATAACTTTTGTTAATTGATAAACTTGCTCAGGTGTAATAAATTGTTGTTTAACTAAATCAAAATTTAATAAATATTTTTCAATTGCATTAGCTAAATAATCAATTTTTCCATGATTTAAAACATCATAAATTAATTGAGATTTTGGACTTAAAAAAAGCATTGGGATATATGAAACATTTTGGAAAGTAGAGTCTTGTTTTACTCAACCATTACCACTTGTTTTAATAAATTTGGTTGCAATAGTTAATTGATATTTTTCCATTCATTTTGACAAAAGACCCTTATCCATACCATTTTGTGGCATTTTTTCTGTATAAACAACTTCTCATGTCTCAGGGCTTACATATTTATTGTTTACATCTTTTTGTCTTCTAACGATTTTGTATTTGCTTCCAAAAAATGCAACACCAAGATTAGTTAAATTATAATCTGGAGTTTTCGAACCATCTTTATTTTGATCGTGTGGAATAAATTGAGTTAAAGGAACAATTTTTGCATTTTTTGTTTCTTTTAATTCACCTGTTTTTTGGTTGTAGTCAATTAATTTTCCAAAATGATATGCAGGTCTAATATACTCAGGATTAGGATATAAGTTACGTCCAATTGATTGTAAAACTAAAGAAGAAACATAAGGTGTAAGTTGATGAGTTTTATAAATGTTGTTCTGGTTTTCGGTTGCTTTATTTAAAATTGAAGGATCATAATATTCATCATATAATTTACCAACATTTAATTTAACACCATCAACTACATAATTCTCATCACCTGTATTAATAAAGTGGAAAACATTTTGTTTTCCTGTTTCCTCATTAATTCCATCAACAGTTAAAGTTTTACGAAGTCCGATATTATCTTGTTTAACTTTTGCTACAATTAAATCAATGATTTTTCTTTTTGTTGATTCATAAGCTTCATTTTTAATAATTGCAAATCTTTCAGATTTAATGTTTTCATTGTTAATTTCATCTAAAAATCTAACTTTATCTTTTAAGAATTGTGATTCTTTTTCAATTTTATTAATTGTTCTAGGAGTGTCTTTTCCAATTAAATATTTTTGACCATCTGGTCTAATTCTTGAATCTTTTGTATATAACTCTAATTGAGTTGTTTCAAAATAATTAAAACTTGAGCCTAATGGAATAACCGATTCTTTTGTAATTTTGTTACTTAAATCTAATGTTTTGTCACTTTTGTATGGGGTAACTATATTCTTTTTACTTTTTCTTCAATATGAAAAAGTTGTTAATTTATCTAACATTTTTTTGTTTTCTGGATTTGTTTTTGAAGACTCAATTAAGTCTTCCATAAATGTTTTGAAAGCACCTAATCATTTATCTTTTGATTTATCTTTATCAACAAAACTTGTGTAGTAGTTTCATCTTAAAAAACGAACTATGTTTTCTTCTTTAGCAATTCATTCAATTGGTAAAGTAAATTTATTATTTTTGTTTAAAGATAATTTCTTTTGAGCTTTTATTGCTTTAGGGTTGGCTGCAAAAACATCTTCAAGATTTCAGTCAGGTTTTAAATTTAAGTTTTCATTTATTTCAGCTCAACTTGGATTATATTTAATAAATTTATCTAAATTGGTTGTTTTATCTTTTTGTTTAAAAAGTAAGCCATTTGGAGTACTGCCATCAAATGAAGTAGGTTCAAAATTAAATAATGCAGCAAGTTGTTTAGGTTCTATTTCAATACCTTTTTCTTGGTTAATTCAACTTTTACCTTTTGAATAGTCTGCTGTTATTTCCTTTGTGTCAGTATTAATAAATAATGAACTTAATTGAGTTGCATAAATTTCATTATTGTTGATAGTTAAATTCATTAAATTACCTATTTTATAGGTTTTATCGAAATGAACAATGTTAGTTCTTTCAAGTTTCTTTTCAAGAAAAACTTGTTCATAAGTATCGCCTTTCTTTTCATAAATATTCAAGAAACGGTTTTTTTGTTTTAGTTCAAAAGTTAAATTATTTATGTCTTTTAAATCAAAAACAATGTCTGGGTTTTTATTATTTTCAATGTTTTGTTCAGCTTTATAAATTGAGTAAAGATTTAATAAGTCATCTTTTTTAAAGTAATAACTATTTACCGAAGGGTCAATATTACCTATTTTATTAAATGGCAAATATTCTCTTTTATCATTGCCAAAAAATAAAACATTTCTTGTAAAGTCTTCATATTCTTTTTTAGTTGAATCGGGCAATGAAGAAGCTTTTACACTTTGATAATCAATTTGATATTCAATTGGTGTATAGTCTTTATTTGTCCCTTTGAAATCATCTGATTTTTCACCATTAATGTAGTAACCTTGATTATATGCACTACCTTGTGTTGGTAAATTTAAGTCAACATTGATATCTTGCAATTTTGATACATGTTTATAATCATTAAAACCATTAACCATTGAAGAGCTAACGTTTGATAGCAAGGTAAAAATTGCTGAAGTTAAAAAAACTAAAATGCTTAGTCCAGCAACTATAACTTTATTTTTAGAGAGAGATTTAAAAACTTCTTTGAATAATCTTCACATACTTTCTCCTAATAATTAATATGTATTATTATAATAAAAAATATTTAATATTAAAGATTTAATTTTTTATAGAAAATTACAAAAATTAATTTTTGTAAAAATTATTAATCAATAGATTATGTTTCTATTTTTAGAATTATCTTTTTTATAGGATTTTTAGTCAAAATTTCAAATAAAAACTTGCTCTTTTAAAGCAAGTAATTATGATTATTTTTTAGAATTATCTTTTTTTGTTTCAGCTTTTGAATCTTCTAATTTTTTGTCATCTTTGTGTTCATTGATAAGCTCATCTAAAGTATAAACTTTGGTTTTGGTTTTGTCAGCTTCTCTTTTTGGAGGAAGTTTCATATTCTTTTCAATGTATTCAATTTCTTCAGCAACAATTGTTTCATTTTCAAGTAAAGCTGTCTTGATTAATTCAAGAAGTTGTCTGTTTTCTTTAATGATTTTAGCGGCATTCTTTTCTGCTTCTGTCATTATTCTTCTAACTTCAAGATCAATTTCGTGGGCTACTTGAGAACTAAATGAAGCACTTTTTAGATAATCACGGCCTAAGAATGGAGAGCTTTCATCTTTTTCATATTGAATAGGGCCAAGTTGTGACATACCAAATTCAGTTACCATTCTACGAGCAATTTTGGTTGCTTTAGAAATATCATCACTAGCGCCTGTAGAAACATTGTCTTTTCCATAAATTAATTCTTCAGATGCACGACCACCCATAAATCCAGTAATCATTGCAATCAATTCTTGTTTTGTTGCATTATATTTTTCTTCTTCGGGTGTCATTAAGTTGTAACCACCGGCATTTCCACGAGGAATAATGGTAATTTTTTGAACTTTGTTTCCACCTTTAACTTTTAATCCAACAACAGCATGACCGGCTTCGTGGTAAGCAACCATTGTAAGTTCTTCTTTAGTAATAACTCTATTTTTCTTAGCAGGTCCAGCCATAACTCTATCGATACCTTCATCAATATCTTCACGTCTAATAACGTCTGAGTTAGCTCTAACTGCTAATAATGAGGCTTCATTAATAACATTTTCAAGTTGAGCGCCAGAAAATCCTGGAGTTCTTTTTGCAACTTGATCAAGTTTAATTTCAGGTGATAATCTTTTACCTTTAGCATGCAATTCTAAAATTTCTTCACGTTCTTTAACATCAGGTAATCCAACTGTAATTGTTCTGTCAAAACGACCTGGACGAGTTAAAGCAGGGTCTAAAACGTCTGTACGGTTAGTTGCTGCAAAAATTAATATTCCATTATTTTGAGTCATACCATCCATTTCAACAAGAAGTTGGTTAAGTGTTTGTTCACGTTCATCATGACCTCCGCCTAAACCAGCTCCACGGGTACGACCAATAGCATCTAATTCATCAATAAAGATAATAGCTGGAGCATTTTTACGAGCTTCTGCAAAAACAGTTCTAACTCTTTTAGCACCTAAACCAACATACATTTCAACAAAGTTTGAAGCTGAAATAAAGTAGAAAGGTACATTTGCTTCACCTGCTGTAGCTTTAGCTAACAATGTTTTACCTGTCCCTGGAGGGCCTCCAAGTAAAATACCATGTGGCATTCTAGCACCAGCAAGTGCATATTTTTTAGGATTTTTAAGATAATCAACAATTTCTTCAATTTCTTCAATTGCTTCTTTGTTACCTGCAACATCTTTAAATGTTTTATCTGATTTGACTTTTCTTGCAGGATTCTTTTCATCTCCAATTGCACCCATCATACTCATATTTTTTTTCATCAAGTATCTGTAAAGGAAAAATAATAAAAGAATAGTAATAAATGTTGGAATTAATGAAGTAAGTAAACTTCTTAATAATGAAGGTTGTCCATCGGGAATAGGTTTAGCTAAGTAAGATCCACCTTTTGTTAGGACAAAAGTTTTTAAGAATTCAAAAAAAGTTAATCCTTTAAAACCAGCTTTATTACCTAAGACATTGGCTAATCCTTCTCCAATAATTTCATTAGTTGAAGATCATGATGCTGCATTAGATCTTCCAATTTGAGCAATTCTTTCAACTAATATGCGGTTACCTTTTAAGTCTAAACCAGCATATACATATTTTGCAGTTCCATAATACGAGTCAAACTCTATACTTGCAAAATAAACATCATCTGATTGACTAGCTGAAGCAGCTTTTAAATCATTCATAAATTGATTTAAGTCAACAATTTGAGGCTTAGGTCCAAATCTTTTAAACAAATAATATGAAACGATTGATAAAGCTAAGAATGACAATACTGTAATTAAAATAATTGACAGTATGCTTGGTTTTTTTTGCATAAATGCTCCTTTCTTAATTTGGTTAATAAATAAAACAAATTATACATTAATATTTATATTAAATAGTAATTTTACTTTTTAGTAATAATTTTCCTTGCGACTTTTGCAAATAATATTGACTGTTTAAAATAAAAATAGGATTATTTGTATTATCTGCAATTACATAATTAACAAGATTTTTAATCTTGCCTGAAGTTAAATTGATTTTATCTTCGCAATGAGCAAAAATAAATTTTCTAATTAATTGCTCTTGAATTTCATAATCAAGATCTTTATAATAATTAACCTTGAATTCTATTTTTTTTCAGCTTGCAAACGCTTTATTAAATTCTTTTTCTAATCATTCTAATTCTTTGTTTTTTTCATTAACTTCATCAATAATTTTTTGTTTTTCTTCGTTAGTTAATTTCTTTAGTTTTAATCTAAATTTATTGCGTGTATAAATAGGCAATTCATTGGTGTAATCAATTGCATAATCTAATTTTAGTTTTTTACATTTTTCTAAAATTTCATCTTTAAAATACTTATCAATAAAAGGTCTTTCAATTAAACAGCCTTTATAAATATTTGTTTTTTTAATGCCTCAAAATGAAGGTTTTCTTTTCGCTACTTTTTGCATAAAAAATGACTCTAAAAAATCATCTTTGTGGTGTGCTAACAAAATTTTAGAAGCATTCTTTTCATGATAATTTTGAATAAAGATTTCCATTCTTTTGTCTCTGGCCCATTCTTCAAAATTGCCTGTTTTATAGTCTTTTTGGTCAAAATTGTATATTTGATAATCTATAAAATTTTGTTTGCAATAATTTTCTACAATTTCCACATCTTTATGTGAATCCATCCTTTGATTATAATTAATGCACACTACAAAAATGGGCTCTTTTTTATAAGCATATTCATGTAAAAGTAGCATTGAATCTGGGCCGCCACTAATTCCTAAAATATACATATTATTTACCTTTACGAAAAACATTAAAGTTATTCATTACTATCTTAATATCATTGTAAACAAAAGATATTGCAGCTTGTGCAGCAACATTTAGAACTTGTTCGACTACTAACATTTGATCACTTGTAAAATTACTTAAAACATAATCTTTTAATTCCATTTTAGGATCTCTTCCGATTCCTATTCTAAGCCTCTTAAATTCATTGCTTCTAAGAGCATTAATTATGCTTTGAACACCATTATGTCCAGCACTTGAACCGCCTACTTTAATTGCTGCTTGACCAATTTCATAATCCTTTTCATCATGAATAATCAAAATATCTGAAGGATCTATTTTATAAAATGAAGCTATCGCTTGAACAAATTCACCAGAATTATTCATATAAGTAGTTGGCTTAGCTAAAATAAAATCTTCAGTTTTTGCATAAAAGCCATTAAATTTTTCTTTTGTAAGAGTTAAATTAAAGTTCGCACAAATTTTGTCGACAGTTAAAAAACCAATATTGTGTTTTGTAAAACGATATTGGCTTCCTGGATTACCTAAACCTACGATTAATTTCATATGTTAATTATAAATTATTTTTATTTTTAAATTCAAAATTCATATTTGTTAAAACATTAATTTCTTTTAGTTTAGGTACTGTTCTTTAAATTCATCTACATCATTCATAATAATGATTTTCTTTTTAGCTCTTGAAAGAGCAGTATATAAGTGATTTTTAGTAATATATTTGGCTATGTTTTTGTCAAAGTAAATGATAACTACATCAGATTCAGAACCTTGAACTTTATAATAATTCAAACAATAAGCTGATAAAAAATCTTCACTTTTTTTATTGTAAATGATCTTTTTTTCTAAGTCATCTTTGTAGAATCTTAATTCAATTAGATCTCTTTTATAATTTTCTAAAGCTTGACCTAATTCACTATTATAAATGTTTAAATGATACTTATTTACATTTTGAATAAAAGCATAAACAAAGGAACTAAATAAATTTTACATTACACCTTGGAAATAAAAATCAATATTATTTAATTTGTGATTAACATCTCTAACTTGTGAATTTAAAGGAAACAAAGCAACTACTTTTTCAACTCAGTTGGTTTTCCTTCTGGATAATTGCACCCATAAAATTCTTTTAGAAGTGCATAAATAACAAAAAGTTCATCTTTATAATTTGGGAAACATTCTTTAATATTTTTTGATGAATCGATAAACTTCGAGTCAATATAATTATAATAATATTTGAGAGCTTCGTATTTTCTAGCCATATCATTTTGATTAAATGCAATTACATTTTCACCATCAAAGTGAAATGGTTTGTTTTCTAAAATACTTTCATAGAATTCTTCAATATTTTTGTTCGCACGGAATGAGCCTTTAAGTTTTAATACTTGTGAATCTTCAGAATAATAATTAATGATCTTTTTTTTATCATCAAAATTGTAATTAATTGAACACAATGTGCCAATTTTAAGATCTTCTAATTCAAATTTGATGTGAAATATAACCTTTTATTTTGTTATAAACCATTTTGTTGATATCAAAAGATTCAAAAACAGCTAAAACACCATTCACGTTGTGATTTTTTAATTTGTTTAAAACCATTCGTTCATTTGTATTAGTTATAAACATTATGAAAATTATAATAAATTATTTATTAATTTTGTTCCTTAATTAAATCAAATAAATATGTTTTTATTTTACTATAATCTTCAGTTTTTGTTTTTTTAGCGTATAAAGCTAAGTCGCCTAAAATAAATAATTTTTCTTTAGCTCTTGAAACTGCTGTATATAAAAGTTTTTTATTAAGCATTCTTGAATAATCTTTAAAAACCGGAATTACAACATGTGGCGCTTCTGAACCTTGAAATTTATGAACAGTTAAAGCATATGCTAAAGATAAATTAATTGCTATTTCTGATTTTACATAATTAACTGTTTTAAATTTATTTCCACCTTGTGGATATTCAACACTAACAATTTTGTCATCTATTATTTCTTTTATTATTCCGATCTCGCCATTAAAAACATTTTTTTCATAATCATTTATTAATTGGATAACATTGTCAAATAAATAGAATTTTGTACCTTCTCAAGATTGGATATAGTTGTCTTTACTTTGTTTAAATTTAGGCAACTTATATGTGTAATTATTTAGTTTTTCTTTTTGCAATCATTTATTAACTGTTTTGGCTCCAACTTGACCATCATGAAGTGGAATCAAGGTTACCATTTTTTCAGAATTGTATTCTGGATTATAAGTTTGATATAAGTATGATAATTCGTTTAATAATGCAACTGAGTTTATTTCTTTCATTTGAATTGTGTTTGTACTTCCTATTTCAACTTCTTTAACTTTTGAATCAATAATACTTAAAAAGTGTTCGCAAATTTCATCACTTTCAGCTCTTTGATTTTCAATTAAAAGAGTTTTAGGAATAAAATCTCAATTACATAAGTCGTGCATTAAATTGCCAGGTCCAATACATGGAAGCTGATCTTTATCTCCAACAATAATGAGCTTTTCAAGCTTGGATAAATGACTTAAAAGTTGATAAAAAATCTTTAAATTAACCATTGAAAACTCGTCAATAATTACAACTTTCGTTTTGTCACAATCATTGTTATAAACAACGTCTTCATCTTCTTTATCAATTTTTAAATAGTTGTGAATCGTTTTTGCAGGCAAATTATTACTCTTATTTTTAATATTAATTGCAGCTCTACCAGTGGGGGTCAAAATTTCTATTTCACCTTTTTTATAGACTTTTTGGGCAATTAATGACTCATAAATATTACTAATTAAATATGTTTTGCCAGTGCCAGGGAATCCTGAAATTATGCTTATCGGTTCATTTAGTGCAATGTTTAAAGCATCAAGTTGTTTAGCAGTTAATGTATCATCTTCTATTTGCGGAATAGTTTTATTTATCTTATTTTTTATAGTTATTAATTTTTCAAAAATAAAGTCTTCTTGTTCTTTAATAAAACTAAGTGCTATAGTTGGAACTTCTTTTTCTGCATAATATAATTTTTGCATTTCAATTAGTTTGTTTATCGAATTAATAAATACATTATCAATAGCAAGCTTTGAAATTGATAACTTATTATTTAGTATTAACTCATTTGTAAATTCAAGTCAATTTTTATGTAAATCAGACAAATTGTAATATGTATCATTAACTTGAATTCCAAAATCATAAATAGATTTATAAACTATTGCATAATATCTAGTTTCGCAATCTTTTGAATATCCTATTAAGCTTGCAAAATTGTCTACTGAGTCAAAACTTAATCCTAATTCAATATACAAAATGAGATAAGGATCTTCATGATTTTCTTTGTTTTTGTCAAAAAAATCTAAATATGATTCGCCATCTGCAAGAGGTAATTTTTGCTTCAATTTAATATAAAAATTGTAAATATTATTGGTTATAAAAAATCTTTCATCTTTGCCCATGTTTAGAACTTCTTCAATAAAAGCTTCTATTTTTTTAAGCAAAGGGGGTTTAATAAATTCGTAAAATATTTTTCTTTCTTCTTTAGGTGTATCTTTAATAACATCTAAATAATTTTCACCTAAATTTTCTTTTAAAATATTATGATCTTCTTCTTTAAAACCTCTAATATGATTCTTCAACATAACTTTTAAATCTTCTTTTGTTGCTTCAATCATATTGTAGCCTTCAAGTATGTAATTATTATATTTATTAGGCCCACTTAATTCCATTTCATATAAATAACCAACTTGTAAAAAGTCATCGGAACTAATTCGAGCATAGACAACAAATAATTGGTTTTCTTGAGATTTAAATTCAAAAAGAATTCACTTTTGAGCCAATCCTTTTCTTATTTTTTTTATTGTTCCTTTTACTTTTATTGTTGTTTTTGCTTGCATATTATTAAATTATATTGAATAATATAAAAATAAATATGCTTAATTTTAAAAAATAAAATAATAAAAACAAAATATAAAATCCACTTTCTAGTTTCATATGCTTGGTAGTTTGTACTTATTATTTTAAACAATGAATTCACTATAAAGATAATTCTTTAGATCTAAATTATAAGTTTTATTCTTATTTGATCACAAATTACCATCATTAACTACTAAATTTTTATATAGTTTTTAGAAATTGAATCTTCTTTTCTTTTGAAACTAAATTTAAATCTAATTGCTGAATTATAGTGGTGTAACATATTTGTAGCTATAAAAGTTGATAAAATTTGAAACACAACATTATTGAGCTCATTTAAATAAGTTTGATATTCATTTTCATTAACTATTTTTTTGTCTTTAAATAAGGATATTATCAAATTTTTAAGTATATATAAAGAATATTCTTATTTTTAAACACAGTTTTCCACATAGTTATAAACATATGTTAATAAGTTAACTTTTCTACTTTTTATACTATAAATAAGA", "length": 977524, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000148625.1|CP001995.1", "orig_description": "HMT-607 Mycoplasmopsis fermentans JER" } ], "run": { "start": "2026-01-26 11:31:08", "end": "2026-01-26 11:38:21", "duration": "7.23 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }