{ "genome": { "genus": "Mycoplasmopsis", "species": "fermentans", "strain": "M64", "taxon": "Mycoplasmopsis fermentans M64", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 1118751, "gc": 0.26859506717759357, "n_ratio": 0.0, "n50": 1118751, "n90": 1118751, "coding_ratio": 0.6582581825625183 }, "features": [ { "type": "oriC", "sequence": "contig_1", "start": 1, "stop": 290, "strand": "?", "product": "origin of replication", "nt": "AACATTATTGAGCTCATTTAAATAAGTTTAATATTCATTTTCATTAACTATTTTTTTGTCTTTAAATAAGGATATTATCAAATTTTTAAGTATATATAAAGAGTATTCTTATTTTTAAACACAGTTTTCCACATAGTTATAAACATATGTTAATAAGTTAACTTTTCTACTTTTTATACTATAAATAAGACTTTTAATTGTTGATAAGTTTGTGGATAACTTGTTTAAATAACTAAAAAACTTACTAATTATTTCTTACATTAAAATATAAATAATACTATTTTTATATT", "id": "EFPKPENHBP_2040" }, { "type": "cds", "sequence": "contig_1", "start": 291, "stop": 461, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAGAATAAAAATATAAAAGATAGTATCACAATGAAAGCAAATACATTAACATTTTTAAAATCTTTAAGTTCAGATGTTAATGACCAAATGTTGTTCAAAAATTTCATTTCAAACTTCACAATTGTAGGCATAGATCAGAACAATCATGTCAAAATTCAAACATGA", "aa": "MAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVGIDQNNHVKIQT", "aa_hexdigest": "e455e33f97f0962e50210e5d426462f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6362.225699999999, "isoelectric_point": 8.12064723968506 }, "id": "EFPKPENHBP_47", "locus": "GCA000186005_00001" }, { "type": "cds", "sequence": "contig_1", "start": 495, "stop": 1235, "strand": "+", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A168RAH0", "UniRef:UniRef90_C4XFM3" ], "nt": "ATGCTAGATGGTAATATTTTAAAAGCTTTAGAAGAAACTTTTGAAAGAGAATGCACATATGAATTTGTTCATATTCCTAAACCTATTGTTAAATTAACAAAAGCCTCAAGCAACAAAACAATTTCTTCTAAAACAATTGAAGAAGAAGTGAAATTAGGCGTTGATTTAAATAAAGAATTAACATTTGACAACTATGTTGAAAGTGATTTTAATAAAGATGCAATCAAATTAGCTAACTATTTAGTTAAAGGTGGAAAAGAATATAATCCTATTTTTATTTATGCTAAAAGTGGACTAGGAAAAACACACTTATTACATGCAATTGGTAACAAATTACAAGAGCAAGGCAAAGTTGTTAAATACATTAATCCAAGCATTTATGCTAGAGATATATCATTCTATTTGCAAGAAAATGATCAAGCAAAATTAAAGAAAATAAAAGATGAATTTGATAATGCTGATTACTTAATATTTGATGATTTTCAAAGTTATGGAGTCGGAAATAAAAAAGCAACAATTCAAACAATTTATAATATTTTAGACTATCGTTTGAACACAAATAAAACAACCATATTTTGTTCGGATAAGCCTATAAATAACTTGAATGCGATGTTTGATGAAAGATTAATTTCTCGTCTTTATATGGGTTTACAACTTGAAATTAAAATTCCACAACAAAGTGATTTATTAAGAGTTTTAGATTACATGCTAGATATTAATGATATGAATCCAGAGGCTTGA", "aa": "MLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLIFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEA", "aa_hexdigest": "437979b0c65b6713510117a09417e7b5", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "both", "pseudogene": { "start": 288, "stop": 1538, "observations": { "mutation": [ 459, 1233 ], "stop": [ 459, 1233 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM3", "query_cov": 1.0, "subject_cov": 0.5222929936305732, "identity": 1.0, "score": 486.0, "evalue": 2.57e-170, "gene_start": 1, "gene_end": 246, "reference_start": 70, "reference_end": 315, "reference_sequence": "MMAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVGIDQNNHVKIQTWLQKPSLEILIKMLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLIFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEAWEKEAKNFVTRNYSTSIRTLIGAITRLKFYKMEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK" }, "paralog": false, "identity": 0.9927884615384616, "score": 807.0, "evalue": 2.92e-299, "description": "Internal stop codon at 459, 1233. Nonsense mutation around 459, 1233.", "psc": { "uniref90_id": "UniRef90_C4XFM3", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A168RAH0", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM3", "UniRef:UniRef50_A0A168RAH0", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A168RAH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RAH0" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "EFPKPENHBP_48", "locus": "GCA000186005_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1326, "stop": 1703, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGGAAATGGCCCGGACTAATTCTAAATATACTGTTGCTGTTGTTAATTCAATATTAAAAGACATGCAACAAACAAAAGAAAATATTACACCAGATTCAATAATAGAATATGTAGCAAAATATTACAAAATTCCTAAAAAAGAAATCTTAGGCAAAAGCAGACAAAAAGATGTCGTTCTAGCAAGACACATAGCTATTTATGTTATAAGAACACAACTGGAAGTTCCATTAGAACAAATTGGTCATATATTTGGTAATAGAGATCACTCAACTATTATTAATGCAATTAGAAAAATAGAAACTGAAAGTGATATGCCAGATAAAACAATTAAAAGAACCATATCAATGATTAGTGATGATATCTACAAATTAAAATAG", "aa": "MEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK", "aa_hexdigest": "367f31c02b7669217b6976ba446b42c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 69, "aa_cov": 0.552, "hmm_cov": 1.0, "evalue": 1.0073555488002122e-27, "score": 92.33539581298828, "start": 31, "stop": 99 } ], "seq_stats": { "molecular_weight": 14478.732099999987, "isoelectric_point": 9.396283149719238 }, "id": "EFPKPENHBP_49", "locus": "GCA000186005_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1834, "stop": 2652, "strand": "+", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_C4XFM2", "UniRef:UniRef90_C4XFM2" ], "nt": "ATGAAATTACACATTAAAAAACAAATTTTAGATGAAACATTGGAAATTGTTTCAAAGTATACAGATCCTATTAATTCATTGTATGGGTTCAGATGTATATTAATTAAAGTAGATAATGAAAAAGTAACATTTTCAGCATCTAATGGAGTTATAAGTATAGTTAAATCACTAGATGTTGAAGATGTGGATATTAAAGTAGAAGAAACAGGAACATTTTTAATTCAAGCAAATATATTTAAAGCTATTATTAAAAAATTAAGTGAAACAATTACTTTAAGTCAAGAATATTCAACAACATTAGAAATATCACAAGGCAATTCAAATTATTCATTAACTACAAACCCAATCAATTCATTTCCACAAATAGAAGAAAATATTAATTTAAAAAAAATTGAAATCGATACAAATGAATTTAAAAAAGCTATTAGATGCACAGCTTTTGCTGTTAGTCAAGACGCTTCATTAGTATATAAATGTATTAATTTTAAATTTAAAAATAATAAATTAAATCTAACAGCTACAGATGCAGCAAGACTTGCATATTACACAATGGATCTTAAAAGTTCGCAAGTAGATAATGAAGAATTTTCAGTAAACAATAAAGATGTTAAAGATTTAATACCTGCAGATGCATCTAAAAAAGTAACATTATTCTTTAATAGAATAAAAATAGGAATTGAATATAAAAATACAGTTATCACTTCAAGAATAGTTGATTTGCCTTACCCAGATGTTGAAAGTTTATTTGCTTCAATAGATATTGAAAATAAAATATTTATTAAAAAAGAAGAAATAAATGATTTAATTAACAAAGTTTGA", "aa": "MKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKKIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPADASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKV", "aa_hexdigest": "a765a82a43c80ac677b227a62e0d1ac2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaN" ], "truncated": "3-prime", "pseudogene": { "start": 1831, "stop": 2940, "observations": { "mutation": [ 2650 ], "stop": [ 2650 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM2", "query_cov": 1.0, "subject_cov": 0.7371273712737128, "identity": 1.0, "score": 515.0, "evalue": 7.92e-183, "gene_start": 1, "gene_end": 272, "reference_start": 2, "reference_end": 273, "reference_sequence": "MMKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKKIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPADASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKVWLGTSDKQNRLEFTINKNEITVLNRLNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR" }, "paralog": false, "identity": 0.994579945799458, "score": 707.0, "evalue": 4.97e-261, "description": "Internal stop codon at 2650. Nonsense mutation around 2650.", "psc": { "uniref90_id": "UniRef90_C4XFM2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_C4XFM2", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM2", "EC:2.7.7.7", "UniRef:UniRef50_C4XFM2", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM2" ], "product": "DNA polymerase III subunit beta" } }, "id": "EFPKPENHBP_50", "locus": "GCA000186005_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2725, "stop": 2940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAATCGGAACATCTATTGCAAAAACAGAAAATTTTAAATTTGAAGGAAAAGCTTTTGAATTTGATATTAACTATCATTTCTTAAAAGATGCTTTAAGTGTGTTTGAAGGTGACGTTTTAATTCTATTTGATAAAAGTGTACAAAAAATTTTAGTAGTTTCAGATTCAAATAAAGAATCTAAACAACTAGTAACTCCTATGAGAAGATAA", "aa": "MNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR", "aa_hexdigest": "3b9f9ef2ee1fbc2738233ee09d95103e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8158.273899999998, "isoelectric_point": 5.727858543395997 }, "id": "EFPKPENHBP_51", "locus": "GCA000186005_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2942, "stop": 3163, "strand": "+", "frame": 2, "gene": null, "product": "S4-like RNA-binding protein", "db_xrefs": [ "KEGG:K14761", "RefSeq:WP_013526579.1", "SO:0001217", "UniParc:UPI0001A8C3D2", "UniRef:UniRef100_C4XFM1", "UniRef:UniRef50_A0A3P8LAR7", "UniRef:UniRef90_C4XFM1" ], "nt": "ATGAATAATGAAATTTATTTAAAAGATGATGTAATTACACTAGGTCAATTATTAAAGAAAATAGGACTTATTGACACAGGCGGATCAGCTAAAGGTTATTTACTTTCTAATAAAATTTTAATAAATAATAGAACACCAAATGGACGTAGTGCAAAAGTTAAAATTGGTGACATTGTGTGGATAAATAATAAAGTTTTTATGATTAAAAAACTTGAAAAATAA", "aa": "MNNEIYLKDDVITLGQLLKKIGLIDTGGSAKGYLLSNKILINNRTPNGRSAKVKIGDIVWINNKVFMIKKLEK", "aa_hexdigest": "9aeb56913a561be2220eaac433b8a17c", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001A8C3D2", "ncbi_nrp_id": "WP_013526579.1", "uniref100_id": "UniRef100_C4XFM1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3D2", "RefSeq:WP_013526579.1", "UniRef:UniRef100_C4XFM1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM1", "uniref90_id": "UniRef90_C4XFM1" }, "psc": { "uniref90_id": "UniRef90_C4XFM1", "uniref50_id": "UniRef50_A0A3P8LAR7", "kegg_orthology_id": "K14761" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LAR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LAR7" ], "product": "S4-like RNA-binding protein" }, "genes": [], "id": "EFPKPENHBP_52", "locus": "GCA000186005_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3319, "stop": 6099, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0", "UniRef:UniRef90_C4XFM0" ], "nt": "ATGAAAAAAATAAAGAAAATAAGTTTGGCTATTGTTCCTGCAATTGCAACAGTAACAACTTTACCGTTGACTACTATTTCATGTCATAAAGATCCTAAGGAAACTCCAAAATCTCTTACTGAAACAGAACTAAATAGTTTATTAGAAAAAATCAAAGTAACTTTATTAGATAAAAATTTAACTGCTGATGAAGTTATAGCTAAAGGCAAAGACATTTTTAAATTTACAGATGTAGATACTAAAGTTAATTTTAAAATTGTATCAATTAAAAAAGGTGCAAATAAAGGTGAAGTTGAATTAACACTTAAAGTTGTTGATAAAAAAGATTCAAATAAATTCTCTAAAGAAAAAACATTTGAAATAAAAACTTTATTAACACCTGATATGGTATTGAATCAAATTAAAGCTGAATATCCAGATCACTTAAATGTGTTTGTTACAGATGCTAATATAAATAAAATTGTTTACTCTAATTTTGACCAAAACACCTTTGAAATAGTTGAATCTAAACTTTTGAGTAAATTTTATTCAACATTAAAATTTGAATTTAAAATTAAAAACAAAATTAGAAATGAAGTTTCTACAAATAAAAGAACTATACAAATTGATGGTTTTAAATTAACAGACAAATATTTAAATTATCAACTCGGAAATATTGAAATTTATTCAGATGGATCTAAAACTTTAAAAGAAACAAAAACTGAACTTTTAAATCCAAATACAACAACATACAAAACAATATTTATTAATAACTTACCATCAACAGTAAAAATTGTTTTTGAATCTGTTGAAGTATATAAAGAAAATGCTGATCAGTTAGAAATTAAATTTCATCTTACAAATATTCACAATGAAAACAATAAATCTAATACTAGAACTGAATACATATATCACAAAAGTGATCATTATGATGCTTTAGTTCAAGAATTTGAAAAAGCTAAATTATCATATGAAGGCGGTACTAACAAAGTAAATGCTTTTGATATTTTTGAAAGCAAAATTAAAATAGAAGGACTTAAAACACCATCAGATTTTTCAGATTTTGTTTTTAAATTCACAAATTCTAGCCCTAGCTCTACTGAATACAAAAGTGGTAAAAGAAAAATTGATTTTAGTTTTGTTTATAAAGGAAACAAATTCTTCAGAAGTGAACAAGAACTTCAAGTAAGACCAACAACGAATGAAATAATTACATCATTAAAAAAACTTAATAAAATTAAACTTGTTGAAACAACTGAAGCTTTAAATAAATTAGAAAGCTTAGTTGATGGTACAACTTTAGATGCATTTAACAATTCTATTTCAATAGATGGGGTTGAATTATTTAGTTACAATTTTACTGCATATCTATTTTCTGAAGATAATGATACTTCAGCTAAAAAAGTATTACTAAATAAAGATAATGAAAACTATTATTTAAGTTTCTACTTAGGTGGAAAAGTATATGGCAAATATGTAAGACACTATGAAGAAAGTAGCACTACTATAGCTAAAATCCAAACACCTGCTGAATTGACAGCAATGGTAAATTCAAAAATTAATGAATTTGATTATAAAAACAAAACAACAACAAAAGCTAAAAATGCCATTCAAGAAAACATTGTTAGCCCAGATTTTGGCTCAGGTTACAGCGTTAAAATTTCAGACTTCACAACTTTAAATGACAAAATTACATTTAATGTTCAATTAAGTAATTCTCAACATCCAGATGTTTTCTCTAATAAAGTTCCAATTACAATTTCAGGTTTCTACCGTAGCGAATTAGCAGAAGAAATCACTAACATGGAAGTTACAATTGCTGATTCATTTAAAGCTAAAGAAGCTAAAGAATTCACAGATGTAAACAACATTACAATCACAGACAAAAGAACAAGTACTGCTTATGATTTTGCAACTAAAGGTGTTTCTCTTGAAACAACTATTGTTGAAGTTAAAAATTTAGAAGGTATTATTAAATTCAACTTAAAATTCACAAAAGAAACTGAAGTTATTAATAAAGAATACATCATTAATGATTTCAAAAAAGAAACTTTAACAGCTGAAAAAATTGTTAAGCTATTAAATATCCAATTAATTAAAGAAAATGTTCAATTAGACAAAAAATACTTCTTGTCTTCATATATTACAAAAAATGAAGTATCTATTACAAACTTAGATGATCTCAAAAAAATAAATTCAACAGTTAATGTTGTTATTAATAAAACAACACCTAATGTTGCTGAAGGTAAATTAGACATTGAATTAAACATTTCGTTTGATTCAAATTCAAATACTAAAACAATTTCAGTCAGTGGCTTTAACAAAAACGTTAAATCTTCAACTGAAAATATGATATTTAAAGACAAAATGAAGTTAATTGTTTACTTGGCAAAAAATAATATTTTGTTCAAACAAGTAGATCAAAACACAATTAATGAAATGAATGTTGCAGGTCCATTTGGCATGACAACATCTATTAAACAAGGCTTAACAAAATATGGTTTTAACCAAATGACAATGGAAAACAACTTCAATTTTGAATATGTATTTAACTTTGTTTCAGCTGATACTGAAACAGTTCAAGATGAAAAATTTGATACTTTAATTAGACATCATATTGTTGGTAATAAATATAATAAAGATCAACCGATGTCTCGCCACATAAAAGTTTTTAGAATAAATGGTAATACACAATTAGCTGCATCATTTAGAATTGTTGCTAGAGATCATGTTAAATTTGATTTAACTAAATTTGAATATAAAACATCAGTTGACGATGATGAATATAAAGTATTTACATGA", "aa": "MKKIKKISLAIVPAIATVTTLPLTTISCHKDPKETPKSLTETELNSLLEKIKVTLLDKNLTADEVIAKGKDIFKFTDVDTKVNFKIVSIKKGANKGEVELTLKVVDKKDSNKFSKEKTFEIKTLLTPDMVLNQIKAEYPDHLNVFVTDANINKIVYSNFDQNTFEIVESKLLSKFYSTLKFEFKIKNKIRNEVSTNKRTIQIDGFKLTDKYLNYQLGNIEIYSDGSKTLKETKTELLNPNTTTYKTIFINNLPSTVKIVFESVEVYKENADQLEIKFHLTNIHNENNKSNTRTEYIYHKSDHYDALVQEFEKAKLSYEGGTNKVNAFDIFESKIKIEGLKTPSDFSDFVFKFTNSSPSSTEYKSGKRKIDFSFVYKGNKFFRSEQELQVRPTTNEIITSLKKLNKIKLVETTEALNKLESLVDGTTLDAFNNSISIDGVELFSYNFTAYLFSEDNDTSAKKVLLNKDNENYYLSFYLGGKVYGKYVRHYEESSTTIAKIQTPAELTAMVNSKINEFDYKNKTTTKAKNAIQENIVSPDFGSGYSVKISDFTTLNDKITFNVQLSNSQHPDVFSNKVPITISGFYRSELAEEITNMEVTIADSFKAKEAKEFTDVNNITITDKRTSTAYDFATKGVSLETTIVEVKNLEGIIKFNLKFTKETEVINKEYIINDFKKETLTAEKIVKLLNIQLIKENVQLDKKYFLSSYITKNEVSITNLDDLKKINSTVNVVINKTTPNVAEGKLDIELNISFDSNSNTKTISVSGFNKNVKSSTENMIFKDKMKLIVYLAKNNILFKQVDQNTINEMNVAGPFGMTTSIKQGLTKYGFNQMTMENNFNFEYVFNFVSADTETVQDEKFDTLIRHHIVGNKYNKDQPMSRHIKVFRINGNTQLAASFRIVARDHVKFDLTKFEYKTSVDDDEYKVFT", "aa_hexdigest": "50863267d315c61d748c9d79f00ce5cb", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XFM0", "query_cov": 1.0896328293736501, "subject_cov": 0.9931102362204725, "identity": 0.915, "score": 1711.0, "evalue": 0.0, "valid": true, "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XFM0" }, "pscc": { "uniref50_id": "UniRef50_C4XFM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0" ], "product": "Lipoprotein-associated type-17 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_53", "locus": "GCA000186005_00007" }, { "type": "cds", "sequence": "contig_1", "start": 6988, "stop": 7200, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354280.1", "SO:0001217", "UniParc:UPI0001E32ED1", "UniRef:UniRef100_C4XFL9", "UniRef:UniRef50_C4XFL9", "UniRef:UniRef90_C4XFL9" ], "nt": "ATGCTATGTCAAACAAGTTATACACAAAGCGTCCAATATTATGAAAAAGCAAATTTTGTTTATATTGTTTTTTCTCTTAAATATGTTTATAGTGATGGCGAAAAAGTATCATTAAAAAATATGACTATTAAAAAATACAAAAAAATCACAAATTATAAAAAAGAACAATTCATTAAAGATAACTTAGAATTGGCAGTTTCGGAGAAATTTTAA", "aa": "MLCQTSYTQSVQYYEKANFVYIVFSLKYVYSDGEKVSLKNMTIKKYKKITNYKKEQFIKDNLELAVSEKF", "aa_hexdigest": "2624e3ad5ee27e47fe5d8d3229bb11f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32ED1", "ncbi_nrp_id": "WP_013354280.1", "uniref100_id": "UniRef100_C4XFL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32ED1", "RefSeq:WP_013354280.1", "UniRef:UniRef100_C4XFL9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFL9", "uniref90_id": "UniRef90_C4XFL9" }, "psc": { "uniref90_id": "UniRef90_C4XFL9", "uniref50_id": "UniRef50_C4XFL9" }, "pscc": { "uniref50_id": "UniRef50_C4XFL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8399.661499999998, "isoelectric_point": 9.301772499084471 }, "id": "EFPKPENHBP_54", "locus": "GCA000186005_00008" }, { "type": "cds", "sequence": "contig_1", "start": 7424, "stop": 7681, "strand": "-", "frame": 3, "gene": null, "product": "CMP/dCMP-type deaminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7", "UniRef:UniRef90_C4XFL7" ], "nt": "ATGAATGCAATTCTAAATACAAACAATAATCTTCAAGGTTGCACAATCTTCACTTCTTTATATCCATGCTCAAGTTGTGCTAAGACCATAGCTCAATCAGGCATTAGTGAAGTAGTTTATGAAGATGACAAATATCATGACACTGAAGACGCAGAAATAGCAAGGCACATTTTAAATGCAAGCAATATTAAAACAAGAAAAATTGAAATAGATACAAATGTTGAAGTTCAAGTGGGTAAAAACAAATACAAAAATTAA", "aa": "MNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN", "aa_hexdigest": "804a9da7e38b9b189bcd6b75ea1f94ac", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 7424, "stop": 7915, "observations": { "mutation": [ 7741, 7894 ], "stop": [ 7741, 7894 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL7", "query_cov": 1.0, "subject_cov": 0.5214723926380368, "identity": 1.0, "score": 167.0, "evalue": 2e-51, "gene_start": 1, "gene_end": 85, "reference_start": 79, "reference_end": 163, "reference_sequence": "MKNKSLYWDGYFMALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFPWDREGESPKETKYAYVVHAEMNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN" }, "paralog": false, "identity": 0.9877300613496932, "score": 324.0, "evalue": 6.19e-116, "description": "Internal stop codon at 7741, 7894. Nonsense mutation around 7741, 7894.", "psc": { "uniref90_id": "UniRef90_C4XFL7", "product": "CMP/dCMP-type deaminase domain-containing protein", "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL7", "UniRef:UniRef50_C4XFL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7" ], "product": "CMP/dCMP-type deaminase domain-containing protein" } }, "id": "EFPKPENHBP_55", "locus": "GCA000186005_00009" }, { "type": "cds", "sequence": "contig_1", "start": 7739, "stop": 7879, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00383.29" ], "nt": "ATGGCTTTAGCTAAAGTATCTGCATTAAGATCTAAAGATCCTTCAACAAAGGTCGGTGCTTGCGTAGTTAATGAAAACAAAAGAATTATTGCTCTAGGTTACAATGGAATGCCAAAAGGCATAGATGACGAATTCCCTTGA", "aa": "MALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFP", "aa_hexdigest": "d7330ea7e3051064131807cc2b734702", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00383.29", "name": "dCMP_cyt_deam_1", "length": 42, "aa_cov": 0.8913043478260869, "hmm_cov": 1.0, "evalue": 2.1246781619299935e-08, "score": 30.32900047302246, "start": 1, "stop": 41 } ], "seq_stats": { "molecular_weight": 4905.6738000000005, "isoelectric_point": 8.978270149230958 }, "id": "EFPKPENHBP_56", "locus": "GCA000186005_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8131, "stop": 8511, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6", "UniRef:UniRef90_C4XFL6" ], "nt": "ATGGCTAAAGATCCTTCAAAAATGGATGAATATCTTAAATACATTGAAGACGGGGGGTTCCACAAATCAATTACCCTTATACCTAAATACAATGATTATTTAGTTAAAGGTGGAAGACTGCATCCTGCTGCCGCAGCCATAGCAAAATTATTTAAAATTGTTCCTTTAATTGCTGTTGATGGTGGTCAACTTAAAAAAGAAGGCGTCGGTCATGTTTTTTCAAAAACCACTATTAAAAATATAGTATCTAAAGCTAAAAACTTCAAAATCAATGACAAGAAAAATGTACTTCTTACTGCTTATATACACACAGGTGCTTCAGAAGAAGAACAAAAAACTTTTATTGAAGAATTCAAAAAACAATACCACCAAGACCCTTGA", "aa": "MAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDP", "aa_hexdigest": "6913da68583222c0c0779a18b0f73455", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "both", "pseudogene": { "start": 7999, "stop": 8811, "observations": { "mutation": [ 8034, 8124, 8133, 8526 ], "stop": [ 8034, 8124, 8133, 8526 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL6", "query_cov": 1.0, "subject_cov": 0.42, "identity": 1.0, "score": 250.0, "evalue": 8.65e-82, "gene_start": 1, "gene_end": 126, "reference_start": 131, "reference_end": 256, "reference_sequence": "MKYAIIVDSSCALTKKQAEALGWFYTPLHINIDEKEFEDGVDINTSNLFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLIWFEQQMAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDPWVNWLAPVVAIHTGPEAFSNVAVECPKETKQIFWEKMDKIKDLL" }, "paralog": false, "identity": 0.9851851851851852, "score": 521.0, "evalue": 9.22e-191, "description": "Internal stop codon at 8034, 8124, 8133, 8526. Nonsense mutation around 8034, 8124, 8133, 8526.", "psc": { "uniref90_id": "UniRef90_C4XFL6", "product": "DegV family protein", "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL6", "UniRef:UniRef50_C4XFL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6" ], "product": "DegV family protein" } }, "id": "EFPKPENHBP_57", "locus": "GCA000186005_00011" }, { "type": "cds", "sequence": "contig_1", "start": 8524, "stop": 8760, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTTTTACACTAAAAAAGCTAAAGTAAAAACATCGGCTATCAATATAGCTAAAACTACAGAATTATTTGAAAAATTGTCAAAAGAGTATGACAAAATTATTTTCTATCCAATCTCTCATCATTTGTCAAGTACTTATCAAACTTCAAAAACAATGGAAGAAGAGTTTCCAAAATTAAGAACAATTAAATCGGTTCAAATTGTTCAATTAATTGTTTTAGATTTAATTTGA", "aa": "MFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLI", "aa_hexdigest": "3284f02f1e1b235258535b2266f4a381", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9159.662299999996, "isoelectric_point": 9.398346138000488 }, "id": "EFPKPENHBP_58", "locus": "GCA000186005_00012" }, { "type": "cds", "sequence": "contig_1", "start": 8871, "stop": 9566, "strand": "+", "frame": 3, "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3CC", "UniRef:UniRef100_C4XFL5", "UniRef:UniRef50_A0A3P8MEK0", "UniRef:UniRef90_C4XFL5" ], "nt": "ATGATGAGTCGACAATTATTGCATATTTCATATTTTATATTATATAATAAAAATATTATGGGTATATTTTTTAATTTAAATTCAAAATTTAAGAACACAAAAATCATATATTTTGATACAACAATAAACAAAAAAACTATGGATATTTATGAAGATATGGTTGTTTTTAGAGATGAAAATTTTAATGTAAATTCGGTTAATTTACTGAATGCAAAAAGTAAAAAAAGAGATGAAAAAATATTTGGTTTTTTAAATGAAAATGAAACTAAAAAACTAGTCAATAAATTGCACAAATATAACAAAAAATATTTACTAAAATCAATTAAATACTTTAGAATTGGTAAAATTGTAGAAAGAAAAAAACACCCAAAAAGCGATAGATTATTTGTATTAAAAATAGATTTTGGAAATGAAGTAAAACAAATAATAACTAACACAACATATACACTTGAAAATAAATATTTTACATTCTGTTTACCAGGATCAATTACAGCTAATGGATTAGAAATTTTAGATGGTAAAATTATGAATGTAGAAAGTGATGGAATGCTTTGCTCAGCAGAATCTTTAGATTTAGAAGCAAAAAGCTATAAAGAATTTGAAGAAGGCTTTTTCAATAATGTTTTAGAAGAAGAAAAACAAAAATATTTAGGCAAAGAAATACAAGAATTATTGCCAGAATTAATAAGCAAATAG", "aa": "MMSRQLLHISYFILYNKNIMGIFFNLNSKFKNTKIIYFDTTINKKTMDIYEDMVVFRDENFNVNSVNLLNAKSKKRDEKIFGFLNENETKKLVNKLHKYNKKYLLKSIKYFRIGKIVERKKHPKSDRLFVLKIDFGNEVKQIITNTTYTLENKYFTFCLPGSITANGLEILDGKIMNVESDGMLCSAESLDLEAKSYKEFEEGFFNNVLEEEKQKYLGKEIQELLPELISK", "aa_hexdigest": "cff29f1e9b65af178a6a0b85e740a348", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C3CC", "uniref100_id": "UniRef100_C4XFL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3CC", "UniRef:UniRef100_C4XFL5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFL5", "uniref90_id": "UniRef90_C4XFL5" }, "psc": { "uniref90_id": "UniRef90_C4XFL5", "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "uniref50_id": "UniRef50_A0A3P8MEK0" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEK0" ], "product": "Phenylalanyl-tRNA synthetase subunit beta" }, "genes": [ "tapR" ], "id": "EFPKPENHBP_59", "locus": "GCA000186005_00013" }, { "type": "cds", "sequence": "contig_1", "start": 9575, "stop": 10072, "strand": "+", "frame": 2, "gene": null, "product": "Tyrosine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "nt": "ATGACAATATTAGAAGATTTAAAATTAAGAGGAATATTAAAGCAAATTAGTAATGAAGATAAGTTTAAAAAACTTGATCCTAAGAAAGTTGCTGTATATTGTGGTTTTGATCCAACTGCGCAAAGTTTGCATTTAGGAAACTATGTTTTAATTTCAGTTTTAAAAAGATTTAAGCAATATGGTTTTAAAGTTTATGGACTTATTGGAGGGGCGACAGGAATGATTGGCGACCCTTCATTTAAAGATAGTGAAAGAGTTCTTTTAGATAATAAAACTTTGAAATTAAATAAAAGTAAAATTAGAAAACAATTAGAAGCTCAAGGCTTAGAAGTAATTGACAACATTCAATTTTATGAAAAAATGAATGTGCTTGAATTCTTAAGAGATGCCGGGAAACTTGTTAATGTTTCATATATGATGACTAAGGATTCAGTTCAAAAAAGAATTGAAAGAGGTTTGTCATTTACTGAATTTTCATATCAAGTTTTACAAGGTTGA", "aa": "MTILEDLKLRGILKQISNEDKFKKLDPKKVAVYCGFDPTAQSLHLGNYVLISVLKRFKQYGFKVYGLIGGATGMIGDPSFKDSERVLLDNKTLKLNKSKIRKQLEAQGLEVIDNIQFYEKMNVLEFLRDAGKLVNVSYMMTKDSVQKRIERGLSFTEFSYQVLQG", "aa_hexdigest": "32e2d6135d2d90e58316d6cf03a8d0e8", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FDW9", "query_cov": 0.9878787878787879, "subject_cov": 0.9878787878787879, "identity": 0.589, "score": 189.0, "evalue": 1.11e-58, "valid": false, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_A0A432FDW9", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FDW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "product": "Tyrosine--tRNA ligase" }, "genes": [], "id": "EFPKPENHBP_60", "locus": "GCA000186005_00014" }, { "type": "cds", "sequence": "contig_1", "start": 10160, "stop": 10273, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTAAAGTTGAGGGTGATGATCATAAAGCTGTTGTAGTTACTGCAGACTTATTAACTGATGAAAATGGAAATAAAATTGGTAAATCAACAGGTGGTGGCAAACTTTGA", "aa": "MISKVEGDDHKAVVVTADLLTDENGNKIGKSTGGGKL", "aa_hexdigest": "c08784d71ee5ffecd1a387dce222ba13", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3798.2368000000006, "isoelectric_point": 5.527671623229979 }, "id": "EFPKPENHBP_61", "locus": "GCA000186005_00015" }, { "type": "cds", "sequence": "contig_1", "start": 10397, "stop": 10810, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGGAAGAACATAATCAAGCACCGTTTAAACATGTAGCTCAAAGAGTAGTAGCTAGTGAAGTAGTTAGAGATATTTTTGGTCAAGATGAATTAGATTCAGCTATTCAAATTACAGAAGTTTTGTTTAACAAAAATTTTGATACAAGCAAACTAAATTTAAAAGATCTTAAATCAATTGAGCAATATTTACCAGTAGTTGAAATTAAGCAAAATCAAAATATTATTGAAACTTTAATTGAAAAAGGTTTTATTGCTTCAAAACGTGAAGCTAGAGAATTTATGGCAACTAAAGCTTTAAAAATAGATGAAGATATAGTTGAAGAAAACTCAGTTTATAAACCAAAAAACTTTTCTAAAAAGTATGCATTCTTTAGAAAAGGAAAGAAACAAACTATACTATTAAAAACTATTTAG", "aa": "MEEHNQAPFKHVAQRVVASEVVRDIFGQDELDSAIQITEVLFNKNFDTSKLNLKDLKSIEQYLPVVEIKQNQNIIETLIEKGFIASKREAREFMATKALKIDEDIVEENSVYKPKNFSKKYAFFRKGKKQTILLKTI", "aa_hexdigest": "1262a29a1076fc2da7cc82ff67a5a347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 81, "aa_cov": 0.5693430656934306, "hmm_cov": 1.0, "evalue": 5.948834893563697e-13, "score": 44.889930725097656, "start": 56, "stop": 133 } ], "seq_stats": { "molecular_weight": 15920.161699999993, "isoelectric_point": 8.70859889984131 }, "id": "EFPKPENHBP_62", "locus": "GCA000186005_00016" }, { "type": "cds", "sequence": "contig_1", "start": 10811, "stop": 11023, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAAAAAGCGGAAAAGACCGCTTTTTTAATTTTGATTACATAAATGTTTCAACTAAAGGAATAATGCCAACTAGTAAACCTGCTGCAAAAGGTGTAGCACCAGGAACAATTGAACCATATACTCAATTTGCTGAAGGAGCTTTAACTACTTCTCCTGTTTTTTCATCTTTTATTCCGTCTTTAAAGAAGAATAATACTGTCATTTGA", "aa": "MIKKSGKDRFFNFDYINVSTKGIMPTSKPAAKGVAPGTIEPYTQFAEGALTTSPVFSSFIPSLKKNNTVI", "aa_hexdigest": "0d53abd1446e1a46912379ffe11964e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7586.693399999997, "isoelectric_point": 9.822999382019042 }, "id": "EFPKPENHBP_63", "locus": "GCA000186005_00017" }, { "type": "cds", "sequence": "contig_1", "start": 11041, "stop": 11169, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTACTTAATAGAAAAATCAAAATTTACTATTTTAGACCCTCTACCTGTGGCATTAGTTATTATGTCTATTGGTATGTCACTTGGTGGTGTAACAGGTTTTGCACTTAATCCTGCAAGAGACTGA", "aa": "MIYLIEKSKFTILDPLPVALVIMSIGMSLGGVTGFALNPARD", "aa_hexdigest": "48487e3f0f6b8ff31ca0e5460d570a3b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4493.3966, "isoelectric_point": 5.980678367614746 }, "id": "EFPKPENHBP_64", "locus": "GCA000186005_00018" }, { "type": "tRNA", "sequence": "contig_1", "start": 11772, "stop": 11845, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 71.4, "nt": "GCCAATTTCGCTTAGTGGTAAAGCAGCTGACTCGTAATTAGCCGCCATGAGTTCGATTCTCATAATTGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 11804, 11806 ], "id": "EFPKPENHBP_1", "locus": "GCA000186005_00019" }, { "type": "cds", "sequence": "contig_1", "start": 12104, "stop": 12289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAATACTTAGAATATTCATTTCAAGTTCTTTTAGGTTTAAATGTAAATGTTACAAATAATATTGTGAAGAGAATTGATAAATCAAATTGGATTGCTGTTGTTATAAAAGCAGTTGATTTGTATTCTTTTTTCATAATACTTTTAATAAATAAATTAAATAACCTTAAGCTGCAATAA", "aa": "MVIKYLEYSFQVLLGLNVNVTNNIVKRIDKSNWIAVVIKAVDLYSFFIILLINKLNNLKLQ", "aa_hexdigest": "811166614d595fe9606d134e30d77b73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7111.4774, "isoelectric_point": 9.698317527770996 }, "id": "EFPKPENHBP_65", "locus": "GCA000186005_00020" }, { "type": "cds", "sequence": "contig_1", "start": 12898, "stop": 12993, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATATTGGATTAAGAAAAGAAGCTAAATATGAAGCAGATTTTGATGATAAAAAAATTAAAGCTTATATTGACTATTTAATTTCTTGA", "aa": "MKKHIGLRKEAKYEADFDDKKIKAYIDYLIS", "aa_hexdigest": "4cc01b3a973439287611d1f38117fe11", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3731.3213000000005, "isoelectric_point": 9.049894523620605 }, "id": "EFPKPENHBP_66", "locus": "GCA000186005_00021" }, { "type": "cds", "sequence": "contig_1", "start": 12994, "stop": 13509, "strand": "+", "frame": 1, "gene": null, "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0", "UniRef:UniRef90_C4XFL0" ], "nt": "ATGAAAGAGAGAGTTGCTGATGCTTGTGCTAAAGGAATTGTTTTAGGTATTAGTGGAGGTATAGATTCTGCTGTAGTTGTCGCCTTAGCAAAAAAAGCTTTTGGCAAAAATGTTCTAGGTGTAATAATGCCTGTTGATAGCATGCAAAATGATTTAAAAGATATCAAAGAATTAGAAAAAGCAGTTGATTTAAAATTTATAACTATTGATCTAAAAAATACCTATGATACTATAAAGCAGGCTTTACCGTTAATTAACAGCAATTTAGCTTTAAGTAATATTAAACCAAGATTGCGTATGGCTGTCATATATGCTTTAGCTCAAGAAAACAATTATTTAGTTGCAGGAACAGGTAATAAATGTGAAACTCATATTGGTTATTTTACAAAATATGGTGATGGCGGAAGTGACATTTTGCCTTTAAGTAAACTGTTAAAAAGTGAAGTAAAAATTATTGCAAAGCACTTAAAAGTGCCAGACTCAATTATTAATAAAAAACCTTCTGCCGGTCTTTGA", "aa": "MKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVIMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPLINSNLALSNIKPRLRMAVIYALAQENNYLVAGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGL", "aa_hexdigest": "422726d1beec85a006952d65c65dff70", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 12880, "stop": 13692, "observations": { "mutation": [ 12991, 13507 ], "stop": [ 12991, 13507 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL0", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 1.0, "score": 327.0, "evalue": 1.61e-111, "gene_start": 1, "gene_end": 171, "reference_start": 39, "reference_end": 209, "reference_sequence": "MKKRGLMKKHIGLRKEAKYEADFDDKKIKAYIDYLISWMKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVIMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPLINSNLALSNIKPRLRMAVIYALAQENNYLVAGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGLWEGQNDEDELGFSYKDLDSYLMDSNAKISQEVKNKIDKMYKNSEHKRNVSLQPQEIDTILK" }, "paralog": false, "identity": 0.9851851851851852, "score": 519.0, "evalue": 1.03e-189, "description": "Internal stop codon at 12991, 13507. Nonsense mutation around 12991, 13507.", "psc": { "uniref90_id": "UniRef90_C4XFL0", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL0", "UniRef:UniRef50_C4XFL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "EFPKPENHBP_67", "locus": "GCA000186005_00022" }, { "type": "cds", "sequence": "contig_1", "start": 13570, "stop": 13692, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTCTAATGCAAAAATTAGTCAAGAGGTCAAAAATAAAATAGACAAAATGTACAAAAATAGTGAACACAAAAGAAATGTTTCATTACAACCTCAAGAAATTGATACAATATTAAAATAA", "aa": "MDSNAKISQEVKNKIDKMYKNSEHKRNVSLQPQEIDTILK", "aa_hexdigest": "8adb8b6f9a2d635929924fbedc3f5072", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.3276000000005, "isoelectric_point": 9.158008003234862 }, "id": "EFPKPENHBP_68", "locus": "GCA000186005_00023" }, { "type": "cds", "sequence": "contig_1", "start": 13796, "stop": 14452, "strand": "+", "frame": 2, "gene": null, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636", "UniRef:UniRef90_A0AB32XA88" ], "nt": "ATGTTTAAAGAAATTTATGTAGCAGCAACAACAGCTGGTGGACCAGATCCAGATATGAACCCAGCACTTAAATTAGCTATTGCAAAAGCTAAAAGTAAAAACATGCCTAAAGCAAATATTGAAAGAGCTTTAGCAAAAGCAAAAGGTGATGGTAAAGATGCATCAGCTTATACAGAAGTTTTATTCAATGCAACAATTGCAGGGGGAGCTACATTTTTAGTTGTTGCCTTAACAGATAATATGAATAGAACAACAAGTTCTATGAAAGCACACTTTAATAGACAAAATGCAGCTTTAGGTAAAACAGGTCAAGTACCTTTTGCTTTCGATCATAAAGGTGTTTTAGAAGTTTCAAAAAACTTAGTTGGAGAAGACGATCTAACTATGGTTGCACTTGAAAATGGTGCCGAAGATTTTGAAGTAACTGAAGATTCTTATATAATTACAACAGCACCAGAAAACTTTTCAAAATGTAAAAGCGCTGTTGAAAGTAACTTAAATATTAGTGATTTTATGCAATGTGAAGTTACATATATTCCTAACACAACAGTTGCTTTTGATAAAGAAAAAAGTGAAAAACTTCAAGAATTTATCGCTCGTTTAGAAGATGACGATGATGTTCAAGAAGTATTTCACAATATTGAATTTGAAGAATAA", "aa": "MFKEIYVAATTAGGPDPDMNPALKLAIAKAKSKNMPKANIERALAKAKGDGKDASAYTEVLFNATIAGGATFLVVALTDNMNRTTSSMKAHFNRQNAALGKTGQVPFAFDHKGVLEVSKNLVGEDDLTMVALENGAEDFEVTEDSYIITTAPENFSKCKSAVESNLNISDFMQCEVTYIPNTTVAFDKEKSEKLQEFIARLEDDDDVQEVFHNIEFEE", "aa_hexdigest": "154d2d629acd25bb20dcb6537374bf95", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XA88", "query_cov": 1.0, "subject_cov": 0.8685258964143426, "identity": 1.0, "score": 412.0, "evalue": 6.02e-145, "valid": true, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q4A636" }, "pscc": { "uniref50_id": "UniRef50_Q4A636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636" ], "product": "Probable transcriptional regulatory protein MS53_0373" }, "genes": [], "id": "EFPKPENHBP_69", "locus": "GCA000186005_00024" }, { "type": "cds", "sequence": "contig_1", "start": 14485, "stop": 14793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATTTATTAGAGCTCTTGAAAATGGTGCTTTAACAATAGGCACAAGAGAAATAGCAGCTAAATTCTATTTGATCTTAATTGCATTTGCAATTATATTTTTAGTGCCAGTTTTAGTGGCATTAATTTTTCCTTTATTTAAGAAAAGCTTAAGTAAAAAAGGAAGTATATTGCTTTATGCTTTTGTAACTGGTTTCTTTTTAACGATGGCTTTATTTGGATTTTTAAAAGAAGCTTTGGAAATAAGTTCAATTAGTGCTCCGGCATCAATGCCACCAGAAGCTTCGTCAGCGCAGATTAAATGA", "aa": "MRKFIRALENGALTIGTREIAAKFYLILIAFAIIFLVPVLVALIFPLFKKSLSKKGSILLYAFVTGFFLTMALFGFLKEALEISSISAPASMPPEASSAQIK", "aa_hexdigest": "89fbce365ff8e912c1f7d7670d63a162", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11144.412399999987, "isoelectric_point": 9.995065498352051 }, "id": "EFPKPENHBP_70", "locus": "GCA000186005_00025" }, { "type": "cds", "sequence": "contig_1", "start": 14869, "stop": 15240, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGTTAAAAAGATAAGTGAAAAACATTTAGCTAAAAGTAAAGCAAGTGTATTTGTGCATACACATGATATGGTTCATAACCATGAACATGGTGAAAATGAAGAACATTCTCATGATCATGTGGCACAAGAACATAAAATATCAACAGACGAAAAAAATGCTAATAACAAACTAAAAGTAGTTGCATTATTGTTGTTATTAACTCACAGAATACCAGAAGGTTTCTTAATTGGTTATTCATTAAATAACTTATATAATCAGAATATTGGCTCATTAAGTATTGCATTCTTTGTTTCATTTATAATGCACTTAATACCTGAAGAAATGGTTTTTTATTATAGACAACGTGAAATGGGTGTAGGTCGTTGA", "aa": "MTVKKISEKHLAKSKASVFVHTHDMVHNHEHGENEEHSHDHVAQEHKISTDEKNANNKLKVVALLLLLTHRIPEGFLIGYSLNNLYNQNIGSLSIAFFVSFIMHLIPEEMVFYYRQREMGVGR", "aa_hexdigest": "9c001a56dc7c38a9eb214bd163165308", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14216.102099999991, "isoelectric_point": 6.864524650573731 }, "id": "EFPKPENHBP_71", "locus": "GCA000186005_00026" }, { "type": "cds", "sequence": "contig_1", "start": 15358, "stop": 15525, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGCTGGTATATTCTTCTTTACAGCTGTGATGGAATTTCTACCTGAGTTTTATCATGCACATCATGACAAAGATTTGTTCAAAAAAGTTATGATTACATTTATGGCTGGACTTGTGGTTTGTGCCTTGATATTAGCATTGCATCAACATGGGACATATAATTAA", "aa": "MIAGIFFFTAVMEFLPEFYHAHHDKDLFKKVMITFMAGLVVCALILALHQHGTYN", "aa_hexdigest": "a8919743e0d3618869803bc5ad767110", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.501399999999, "isoelectric_point": 6.476882743835449 }, "id": "EFPKPENHBP_72", "locus": "GCA000186005_00027" }, { "type": "cds", "sequence": "contig_1", "start": 15719, "stop": 16123, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAGATTTGTTAACGTTGTAGATTTACTTAAATTAAGACATCCAAAAATTGACCCACGCGGTTTAAGTGATGAAGAATTTGATGAAATCTTTACAAAAGACAAACCAGTATTATTTGCTTTCCATGGTTTTGAAGGTATTTTAAGAGACATCTTCTTTGACCGTCACAACCACAATTTAATTGCTCACGGTTATAGAGAAAATGGTGACATTACAACATCATTCGACATTCGTCAATTAAGTCACATGGACAGATTCCACATGGCTAGCGATGCTGCTGCAGCTGTATTCGGATCATCAAAAGCAAAAGAATTCATGGACAAAATGGAAGAAACAATCCAATTCCACAACAAATACATTCGTGAAGTTGGTACAGATATTCCTGAAGTTAAAAACTGA", "aa": "MKIRFVNVVDLLKLRHPKIDPRGLSDEEFDEIFTKDKPVLFAFHGFEGILRDIFFDRHNHNLIAHGYRENGDITTSFDIRQLSHMDRFHMASDAAAAVFGSSKAKEFMDKMEETIQFHNKYIREVGTDIPEVKN", "aa_hexdigest": "841dfb96fb36c35c9497b9abd1ba2ec1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 1.0, "subject_cov": 0.8072289156626506, "identity": 0.657, "score": 192.0, "evalue": 2.08e-60, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15654.597799999987, "isoelectric_point": 6.0416666030883786 }, "id": "EFPKPENHBP_73", "locus": "GCA000186005_00028" }, { "type": "cds", "sequence": "contig_1", "start": 16238, "stop": 16507, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACTTCAACTGCTTTCCAACAAGACCACAATGGTTATACACACCAAGACCCAGGTATTATTGGACACATGGCAGATAAAAGACCAGAACTAATTAGAGAATACTTACCAGCTGATACAAACACATTATTAGCCGTTATGGATAAAGCATTTACAGAAAGAAATGTAATTAACTTAATCGTTTCTTCAAAACAACCAAGACACCAATTCTACTCAGTAGAAGAAGCTGAAACATTGGTTGAAAAAGGTTTAGACATTATTGATTGA", "aa": "MATSTAFQQDHNGYTHQDPGIIGHMADKRPELIREYLPADTNTLLAVMDKAFTERNVINLIVSSKQPRHQFYSVEEAETLVEKGLDIID", "aa_hexdigest": "74af9989c280f6b35af6fe415926b4db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10085.208899999996, "isoelectric_point": 5.021747779846191 }, "id": "EFPKPENHBP_74", "locus": "GCA000186005_00029" }, { "type": "cds", "sequence": "contig_1", "start": 16562, "stop": 16753, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAATTGATTCTAAATTAGATGAAGCTCTTAGTCCAGTAGGTAGAATCATTGACTCACAACTTTCAGAACACCAAGCTCAAGGTTTCTTAGAAGGTTATGTATTAACAGGTCGTCATGGTATCTTTGCTTCATATGAATCATTCTTAAGAGTTGTTGACTCAATGGTTACACAACACATGAAATGA", "aa": "MERIDSKLDEALSPVGRIIDSQLSEHQAQGFLEGYVLTGRHGIFASYESFLRVVDSMVTQHMK", "aa_hexdigest": "956d56023b3219d2e85c34ae7d915f9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7124.998099999999, "isoelectric_point": 5.415187263488768 }, "id": "EFPKPENHBP_75", "locus": "GCA000186005_00030" }, { "type": "cds", "sequence": "contig_1", "start": 16754, "stop": 16882, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAATTAGGAACATACTTTGCTGACTTAGTAAAAAGAAATCCAGAAAACTTTAGAATTTTTGGACCAGATGAAACTAAATCAAACCGTCTTTACAACTTATTCAAAGTTACAAACCGTCAATGA", "aa": "MVELGTYFADLVKRNPENFRIFGPDETKSNRLYNLFKVTNRQ", "aa_hexdigest": "214a769cc8436e2b93a27ee96b92b44a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5022.652500000001, "isoelectric_point": 9.394735908508299 }, "id": "EFPKPENHBP_76", "locus": "GCA000186005_00031" }, { "type": "cds", "sequence": "contig_1", "start": 16989, "stop": 17285, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCATTCTTCTTTTGCCTTTAGGAGCAATAGCAGCGATTTCTTTTACCAATTTACCTTTTTTATCAAATAATTCTTCAGGACGATATGATCTTAATCATTTTTCAAGTGCATCTGCATGTTCCATGTTTTCTGCATTAATTGGAAGTGGAACTTGGTGTGCACGGAAGCTTCCTTCAATAGCTTCTTTGTTTCATGATTTAGGACCTGTTCAACCTTTAGGTGTTCTAAGAACGATCATTGGTCATACTGGTCTTTTAGCTTTGTCAGCTGGTTTTTTACGAGCTTTTGTTTGA", "aa": "MPILLLPLGAIAAISFTNLPFLSNNSSGRYDLNHFSSASACSMFSALIGSGTWCARKLPSIASLFHDLGPVQPLGVLRTIIGHTGLLALSAGFLRAFV", "aa_hexdigest": "be327aa5fc9f75f675274ea0a8aac226", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10272.94589999999, "isoelectric_point": 9.495048713684085 }, "id": "EFPKPENHBP_77", "locus": "GCA000186005_00032" }, { "type": "cds", "sequence": "contig_1", "start": 17234, "stop": 17341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTAATGGCAAAAACTTTAGACTCAGTTATTGAAGAAATTCAAAGCATTCAAACAAAAGCTCGTAAAAAACCAGCTGACAAAGCTAAAAGACCAGTATGA", "aa": "MHELMAKTLDSVIEEIQSIQTKARKKPADKAKRPV", "aa_hexdigest": "03a2515dbd7b44d45134cf5fd3f995f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3991.6814000000013, "isoelectric_point": 9.696447944641115 }, "id": "EFPKPENHBP_78", "locus": "GCA000186005_00033" }, { "type": "cds", "sequence": "contig_1", "start": 17522, "stop": 17839, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTACATTGAAGGACCAGGACATGGTGGCCAAGTTATGACATCTAACTCATACTTAGATGGAAGTTACACAGAATTATTCCCACATGTAACACAAGATGAAGCCGGAATGCAACATTTATTTAAATACTTCTCATTCCCTGGTGGAACAGCTTCACATGCTGCTCCTGAAACCCCTGGATCAATTCATGAAGGTGGAGAATTAGGTTACTCAATTTCACACGCAACAGGTGCAATTTTAGATAACCCTGATGTTATTGCTGCTACTATCGTTGGTGATGGTGAAGCTGAAACAGGACCTCTTGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMTSNSYLDGSYTELFPHVTQDEAGMQHLFKYFSFPGGTASHAAPETPGSIHEGGELGYSISHATGAILDNPDVIAATIVGDGEAETGPLATS", "aa_hexdigest": "abaf62c10f830fc60a38ce8589f21cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10886.74039999999, "isoelectric_point": 4.366280555725097 }, "id": "EFPKPENHBP_79", "locus": "GCA000186005_00034" }, { "type": "cds", "sequence": "contig_1", "start": 18310, "stop": 18627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACAAACCAAATTTCGGTTGTTCCTAAACCTGCTAATGTTGCAGTTAGTGACAAACTATACATGCTTGAGCCTTTAACTCAAATTACTGAAAAGGATTTAGAATTCTTTAACTCAAAAGATTTTAGACACTTTTATCAAATAGCCACAAACTTTTCATCAAGATCAAGCTCAGTTGATACATCAAGTGTATTCTACTTCGGTTTGCTTCAAGAAGATGATGTTGAAACAAACAAAAAACAAAGCGAAACTCAAGAATACGATTTCTCAGATGACTTTGATATTGACACCAAAGAATACGAAAGCAGTTTCTAA", "aa": "MNTNQISVVPKPANVAVSDKLYMLEPLTQITEKDLEFFNSKDFRHFYQIATNFSSRSSSVDTSSVFYFGLLQEDDVETNKKQSETQEYDFSDDFDIDTKEYESSF", "aa_hexdigest": "c283a4e7094f8b701bc0531b84b86c42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12211.143299999992, "isoelectric_point": 4.2429399490356445 }, "id": "EFPKPENHBP_80", "locus": "GCA000186005_00035" }, { "type": "cds", "sequence": "contig_1", "start": 19162, "stop": 19752, "strand": "-", "frame": 1, "gene": null, "product": "DNA methylase adenine-specific domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "nt": "ATGTTTGGTTCACTAACTGAAAGAAACATAAAAGATATCATCAAAAAAGAAAAATTAACAGATGTTAAATATTTAATTAAATATATTGAAAAAAATCCAAAACTTATGGATGTTAGATCAATGTACCAAGTAGCTCAATATGCAAACTGCTTGAGATTAGGTCAAAAACCTTTTTATACAGATGAAAGTTTACTAAACTCAATTTACAAAAGACTTCCAAATATTAGAGGCAAAGAATTAACGATTGTTGATCCTTTATGTGGTGTTGGTATTTTCTTACCTTTAATTATTGAAAAATATGGAGACAAAGACAAACTAAGTATTACTTTTTATGATGTAGATAATGAAACAATAAATATTGCTCGCGCTTTATCTAAAATGATAAAACTTAAAGTTACTTTTGGTGTTAGTTTTCAAAATGCTGATTTTCTAATGAAAAGAACTAATGAACCAATTGACTTATTAATTGGTGATTTCCCTAACATAAGAATGTCTCATCCAGCTTTAAGAAAAAAATATCCTTCATTAACTAAAGAAAACAAATTATTACAAAGTTTTGCTCCATTATACTTAATGCAAAGTAAAAAATGA", "aa": "MFGSLTERNIKDIIKKEKLTDVKYLIKYIEKNPKLMDVRSMYQVAQYANCLRLGQKPFYTDESLLNSIYKRLPNIRGKELTIVDPLCGVGIFLPLIIEKYGDKDKLSITFYDVDNETINIARALSKMIKLKVTFGVSFQNADFLMKRTNEPIDLLIGDFPNIRMSHPALRKKYPSLTKENKLLQSFAPLYLMQSKK", "aa_hexdigest": "a4736865b936e010739a379ee8d7e4b1", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8L726", "query_cov": 0.8775510204081632, "subject_cov": 0.9398907103825137, "identity": 0.855, "score": 297.0, "evalue": 1.16e-100, "valid": false, "product": "DNA methylase adenine-specific domain-containing protein", "uniref50_id": "UniRef50_A0A3P8L726" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L726", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "product": "DNA methylase adenine-specific domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_81", "locus": "GCA000186005_00036" }, { "type": "tRNA", "sequence": "contig_1", "start": 20043, "stop": 20126, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 20049, 20051 ], "id": "EFPKPENHBP_2", "locus": "GCA000186005_00037" }, { "type": "tRNA", "sequence": "contig_1", "start": 20130, "stop": 20204, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 64.1, "nt": "TGCCCCATAGCCAAGCGGTAAGGCAACGGGTTTTGGTTCCGTCATGCGTTAGTTCGAATCTAACTGGGGCAACCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 20162, 20164 ], "id": "EFPKPENHBP_3", "locus": "GCA000186005_00038" }, { "type": "cds", "sequence": "contig_1", "start": 20509, "stop": 20631, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAGGACCTAGAATGCATATAGGTGTATCCATACCTATGGTTATGATCATAATGATGGGATTTGGTCTATTTGCGCTTTCTATTCCTCTTTACTATGCAAAAGAAACCATGGTTTGA", "aa": "MFLGPRMHIGVSIPMVMIIMMGFGLFALSIPLYYAKETMV", "aa_hexdigest": "cfb0ab3d9054e9ace7fcd6ff0300b101", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4507.642900000001, "isoelectric_point": 8.274855613708496 }, "id": "EFPKPENHBP_82", "locus": "GCA000186005_00039" }, { "type": "cds", "sequence": "contig_1", "start": 20902, "stop": 21027, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATGAAGAAACCAAAAAAGAAATGATAAAATTATCTCTTTTCTTTGGTTTCAAATTAGCATATGATTATATTTATTGTGTACTTTATTTAATTAGATTATTGTCAAATATGAAAAATTAA", "aa": "MDDEETKKEMIKLSLFFGFKLAYDYIYCVLYLIRLLSNMKN", "aa_hexdigest": "54551c09f10e2b39f7a050e4f48a271d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5000.9117, "isoelectric_point": 6.057411003112793 }, "id": "EFPKPENHBP_83", "locus": "GCA000186005_00040" }, { "type": "cds", "sequence": "contig_1", "start": 21342, "stop": 21821, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4", "UniRef:UniRef90_C4XFK4" ], "nt": "ATGAATAAAAAATTTTTAAAATTAGGCTCAATTGCAGGAATTTTATCTTTTGCACCAGTAGCAATTAGTGCTGGATGTGGTTCTAAAGGTGGAGAAACCCCTACTGATCCAGTTGCTGCTAAGAAAGCATTAGTTGAACAAGCATTAAAAGATTTAAATGCTAAAATTGAAACTGTTACTGATGAAACTAAAAAAGCTGAACTTAAAAAGGAAGCAGAAGCTATTAAAAAAGATTTCGATGCTGCTAAAACAGTTAAAGATTTTGAAGCTGTAGATGCAAAAATTAAAAAAGTTGTTGCTAAGGTTGAAAGTAAAAGTACATCTGACCAAGACAAATATAAAACATGCTTTACTTTAGGAGAAGGCAAAGATAGAATTAAAGAAGCTATTGAAGCCATAAAAAAAGATTCTAAATCATATTCATTTGCATATGATCGTAAAGTCCACAACATTGTTTGCTTTAAAGGAAGAATTAATTGA", "aa": "MNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDPVAAKKALVEQALKDLNAKIETVTDETKKAELKKEAEAIKKDFDAAKTVKDFEAVDAKIKKVVAKVESKSTSDQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKVHNIVCFKGRIN", "aa_hexdigest": "9cabec73935897851bbeea1d8b8e611f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 21087, "stop": 21848, "observations": { "mutation": [ 21344 ], "stop": [ 21344 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK4", "query_cov": 1.0, "subject_cov": 0.6284584980237155, "identity": 0.9940000000000001, "score": 292.0, "evalue": 1.94e-98, "gene_start": 1, "gene_end": 159, "reference_start": 10, "reference_end": 168, "reference_sequence": "MVKIMRRKSMNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDPVAAKKALVEQALKDLNAKIETVTDETKKAELKKEAEAIKKDFDAAKTVKDFEAVDAKIKKVVAKVESKSTSDQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKGHNIVCFKGRINWSNGDKVIALNITGALKEHIEKNSVQLANAKKPTYNSKKGESISSILDFKLEGNTVKFSFKLAKFENNKPVLDDPIVYNAEVSLA" }, "paralog": false, "identity": 0.9881422924901185, "score": 482.0, "evalue": 1.01e-175, "description": "Internal stop codon at 21344. Nonsense mutation around 21344.", "psc": { "uniref90_id": "UniRef90_C4XFK4", "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK4", "UniRef:UniRef50_C4XFK4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17319.835999999996, "isoelectric_point": 9.341291618347167 }, "id": "EFPKPENHBP_84", "locus": "GCA000186005_00041" }, { "type": "tRNA", "sequence": "contig_1", "start": 21904, "stop": 21981, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 67.5, "nt": "GTCCTTGTAGCTCAGATGGATCAGAGCACGAGCCTCCTAAGCTTGGGGTGGGTGGTTCAATTCCACTCAAGGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 21977, 21979 ], "id": "EFPKPENHBP_36", "locus": "GCA000186005_00042" }, { "type": "tRNA", "sequence": "contig_1", "start": 22020, "stop": 22096, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 64.3, "nt": "GGTCGCGTAGCTCAGTAGGATAGAGCACGAGCCTTCTAAGCCCGTGGTCAGAGGTTCGAATCCTCTCGTGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 22060, 22062 ], "id": "EFPKPENHBP_35", "locus": "GCA000186005_00043" }, { "type": "cds", "sequence": "contig_1", "start": 22519, "stop": 22710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAM5", "UniRef:UniRef90_A0AB32XAM5", "PFAM:PF13518.12" ], "nt": "ATGCTTGATCAAAAGATAAAAATTGTCGAATTATATCTTCGAGAATTTAGTATTTTAGAAATATCTAAAATAATGAATAACTCTTATTCAACATGCTATTCAGTGATAGAGAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATGAATTTAAAGTTATAA", "aa": "MLDQKIKIVELYLREFSILEISKIMNNSYSTCYSVIEKYKKFGYNSFAMEKKKGRKSKMNLKL", "aa_hexdigest": "abe104260aa19d2c84c2d30157ba603a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 22432, "stop": 22710, "observations": { "mutation": [ 22486 ], "stop": [ 22486 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XAM5", "query_cov": 1.0, "subject_cov": 0.6847826086956522, "identity": 1.0, "score": 119.0, "evalue": 1.01e-33, "gene_start": 1, "gene_end": 63, "reference_start": 30, "reference_end": 92, "reference_sequence": "MESDSFLCNSNLKKKKNKWNREIKGYLKLMLDQKIKIVELYLREFSILEISKIMNNSYSTCYSVIEKYKKFGYNSFAMEKKKGRKSKMNLKL" }, "paralog": false, "identity": 0.9782608695652174, "score": 179.0, "evalue": 3.43e-60, "description": "Internal stop codon at 22486. Nonsense mutation around 22486.", "psc": { "uniref90_id": "UniRef90_A0AB32XAM5", "uniref50_id": "UniRef50_A0AB32XAM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XAM5", "UniRef:UniRef50_A0AB32XAM5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XAM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAM5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF13518.12", "name": "HTH_28", "length": 41, "aa_cov": 0.6507936507936508, "hmm_cov": 1.0, "evalue": 1.4336973979814985e-07, "score": 27.81732177734375, "start": 4, "stop": 44 } ], "seq_stats": { "molecular_weight": 7550.944300000001, "isoelectric_point": 9.752406501770018 }, "id": "EFPKPENHBP_85", "locus": "GCA000186005_00044" }, { "type": "cds", "sequence": "contig_1", "start": 22828, "stop": 23154, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATACACAGAACAAGAACAAATTCGTCGTGATAAACTAGAATTTTATAAAAAATTTGATGTAGTACCTTTTAAAAAAGCTTATGGTCTAGGTAAATTGAGTACAAGTGATGAACTTAATAAAAAATATGGTCAATTTGCTAGAGAAGAATTAGAAGCTAAAAAAGTTAAGAAAAATATTAGTGGTAGATTAATTACAGCTAGAGGACCTTTCTTAGTATTAAAAGATCGTAAAGGAACAATTCAAGTTTATTTCAATAAAAAAGAAAATCCTGAATTAACTAAAATAGTTGAAACATTTGACTTAGGGGACATTTTATGA", "aa": "MAKYTEQEQIRRDKLEFYKKFDVVPFKKAYGLGKLSTSDELNKKYGQFAREELEAKKVKKNISGRLITARGPFLVLKDRKGTIQVYFNKKENPELTKIVETFDLGDIL", "aa_hexdigest": "7f6fb2e8bd9d4880b9bf1a1bf91ff176", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12622.515799999983, "isoelectric_point": 9.638877677917481 }, "id": "EFPKPENHBP_86", "locus": "GCA000186005_00045" }, { "type": "cds", "sequence": "contig_1", "start": 23170, "stop": 23364, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "nt": "ATGAAAACTCATACAGGTGAAATGACAGTTAGGGCTCAAGATATTCAATTATTAACTAAAGCTCTTAAACCCCTTCCAGATAAATTTCATGGATTAACTGATACTGAAGAAAGATATCGTCACAGATACTTAGATTTAATTACTAATCCAGAATCAAGAAATACATTCATTATGAGAACAAAAATTGTTCAATGA", "aa": "MKTHTGEMTVRAQDIQLLTKALKPLPDKFHGLTDTEERYRHRYLDLITNPESRNTFIMRTKIVQ", "aa_hexdigest": "6f306cee6dd0378974b3e25ee4b1629a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00257604EB", "query_cov": 0.96875, "subject_cov": 0.8378378378378378, "identity": 0.629, "score": 85.9, "evalue": 8.26e-21, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00257604EB" }, "pscc": { "uniref50_id": "UniRef50_UPI00257604EB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "EFPKPENHBP_87", "locus": "GCA000186005_00046" }, { "type": "cds", "sequence": "contig_1", "start": 23416, "stop": 24294, "strand": "+", "frame": 1, "gene": null, "product": "Lysine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "nt": "TTGCATGATTATCTTTCAGGAGCAGCTGCTAAACCATTTACTACACATCATAATTCATTGAACCAAGATTTTGTTTTACGAATAGCAACAGAAATTCCGCTTAAAAAATTAGTTGTTGGTGGATTTGAAAGAGTTTATGAAATGGGTCGCATATTCAGAAATGAAGGATACGACACAACACATAACCCTGAATTTACAACTATTGAATTTTATGAAGCTTATTCAAATGTTGAAGGTATGATGAATAGAACTGAAACATTAATTAAAGAACTTTGCAAAAAAATTGGTAAAAGTAAATTTGTAACAAATGGTGTTGAAGTTGATTTATCAAAACCATTTAAGCGTGTAAATATGATTGATGAAGTAAGCAAGAAAACTGGTAAAAACTTTAGAAAAATTACTTTAGAAGAAGCTATTGAAGTAGCTAAAGCTTACAAAATCAAAATCGAAAAATTCTTTACTATTGGACACATTATTAATGCTTTATATGAAGAATTAGTTGAACCAACATTAATTCAACCTACATTCTTATATGGACATCCAATTGAAATTTCTCCTTTAAGTGCTAAGAGTGATGATCCAAGATTTACTGAACGTGCTGAATTATTTATTAACACAAAAGAATATGCCAACATGTATACAGAATTAAGTGATCCAATTGACCAATTAGAAAGATTCGAAAGTCAATTAGAAGAAAAGAATAAAGGTAATGATGAAGCAAGTGATATTGACTATGACTTTGTTGATGCTTTAGAATATGGCATGCCTCCAACAGGTGGTTGTGGAATTGGAATTGACCGTTTAGTTATGTTATTAACTGAAACAGATTCTATTAGAGATGTACTTTTATTTCCCACACTAAAAAGAATCAAAAAATAA", "aa": "MHDYLSGAAAKPFTTHHNSLNQDFVLRIATEIPLKKLVVGGFERVYEMGRIFRNEGYDTTHNPEFTTIEFYEAYSNVEGMMNRTETLIKELCKKIGKSKFVTNGVEVDLSKPFKRVNMIDEVSKKTGKNFRKITLEEAIEVAKAYKIKIEKFFTIGHIINALYEELVEPTLIQPTFLYGHPIEISPLSAKSDDPRFTERAELFINTKEYANMYTELSDPIDQLERFESQLEEKNKGNDEASDIDYDFVDALEYGMPPTGGCGIGIDRLVMLLTETDSIRDVLLFPTLKRIKK", "aa_hexdigest": "34de8cf3f4ef36d74884392ef4f02aaa", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0030049D44", "query_cov": 0.9794520547945206, "subject_cov": 0.9225806451612903, "identity": 0.6709999999999999, "score": 385.0, "evalue": 5.81e-132, "valid": false, "gene": "lysS", "product": "lysine--tRNA ligase", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A1V5HK23", "kegg_orthology_id": "K04567", "go_ids": [ "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HK23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "product": "Lysine--tRNA ligase" }, "genes": [], "id": "EFPKPENHBP_88", "locus": "GCA000186005_00047" }, { "type": "cds", "sequence": "contig_1", "start": 25633, "stop": 25965, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02126.24" ], "nt": "ATGAAGAAAGAAAAATTTGTTAGGACTGTGCTAGGCGATGTGCCTGCTAGTTCTATCGGTATTACTGACTGTCACGACCACCTTATAAAGAACGGTGGCCCTGAAATGGAAGAACACATTGACTTTTTAATGATCGATGTTGAAGCTGCTAAAAAAGAACTTCAAGAATTCGTTGATCACGGTGGAAAAACAATGGTTACAATGGATCCACCTAATGTTGGACGTGATGTATTTAGAATGATGGAAATTGCTAATGCATTCAAAGGCAAAGCAAACATTATTATGTCTACAGGTTTCCACAAAGCTAAATTCTATGACAAATACTGTTCATGA", "aa": "MKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCS", "aa_hexdigest": "14467491478e05d57b812bd0a5b99b68", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02126.24", "name": "PTE", "length": 92, "aa_cov": 0.8090909090909091, "hmm_cov": 0.9782608695652174, "evalue": 3.375119633359479e-07, "score": 26.286685943603516, "start": 17, "stop": 105 } ], "seq_stats": { "molecular_weight": 12380.227199999987, "isoelectric_point": 6.1835935592651365 }, "id": "EFPKPENHBP_89", "locus": "GCA000186005_00048" }, { "type": "cds", "sequence": "contig_1", "start": 25999, "stop": 26694, "strand": "+", "frame": 1, "gene": null, "product": "phospho-furanose lactonase", "db_xrefs": [ "EC:3.1.1.25", "GO:0050490", "SO:0001217", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_C4XFK1" ], "nt": "ATGTGTGTCGCAGAAATTGAAGAAGGTATGGACGTTTACAACTATAATGGACCAGTTGTAAAACGTGGTAAAGCTAAAGCTGGTATTATTAAAGCTGGTACAGGTTATGCTGCTATTGACCGTCTTGAATTAAAAGCACTTGAAGTTGCTGCTAGAACCTCAATTACAACAGGTTGCCCAATTTTAGTACATACTCAATTAGGTACAATGGCTTTAGAAGTTGCACAACACTTAATTGACTTTGGTGCAAACCCTCGTAAAATTCAAATTTCTCACTTAAATAAAAACCCAGACCCATACTACTATGAAAAAGTTATTAAAGATACAGGCGTTACACTTTGTCTTGATGGACCAGACAGAGTTAAATACTACCCTGACTCAACTCTTGCAGATAACATTAAATACTTAGTAGACCAAGGTCTTCAAAAACATATAACATTAAGCCTTGATGCTGGTAGAATTCTATACCAACGTAACTATGGTTTAACAAAAGGTAAAGAAACATTTGGATTCTCATACTTATTTGATAGATTTATTCCTTTACTTAAAAAAGTTGGTGTTCCTCAAGATGCTATTGATGACATTTTGATCAACAACCCACGTGAAATTCTTGCATTTGATGAACCTCGTAAATATGAACCATCAAAAGTATCAAACGAATTAAAACAACTTAAAAAAAATCTAAAACTAGATTAA", "aa": "MCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPREILAFDEPRKYEPSKVSNELKQLKKNLKLD", "aa_hexdigest": "3645091e774248648b79842571d6af99", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 25699, "stop": 26694, "observations": { "mutation": [ 25963 ], "stop": [ 25963 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK1", "query_cov": 1.0, "subject_cov": 0.6434540389972145, "identity": 1.0, "score": 464.0, "evalue": 2.65e-163, "gene_start": 1, "gene_end": 231, "reference_start": 129, "reference_end": 359, "reference_sequence": "MQKRRFMKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCSWLANVPTDDIVKMCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPREILAFDEPRKYEPSKVSNELKQLKKNLKLD" }, "paralog": false, "identity": 0.9969788519637462, "score": 669.0, "evalue": 1.17e-246, "description": "Internal stop codon at 25963. Nonsense mutation around 25963.", "psc": { "uniref90_id": "UniRef90_C4XFK1", "product": "phospho-furanose lactonase", "ec_ids": [ "3.1.1.25" ], "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0050490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK1", "EC:3.1.1.25", "UniRef:UniRef50_Q4A724", "GO:0050490" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" } }, "id": "EFPKPENHBP_90", "locus": "GCA000186005_00049" }, { "type": "cds", "sequence": "contig_1", "start": 27113, "stop": 27286, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTGTCTTAATGATGCAAATCGGTTCAGGAATGATTGTTGGTTTAGCTAAACCAGTGTTCCTTGCATTCTCTAAATTCGGTAAAGGGCTTACACCACTTGATACATACTTAGGACAAGTTTCAGTAGAAAACTTCCTTAACTCAGTTGGTCAAGGTTTTGCTTCATGA", "aa": "MIGVLMMQIGSGMIVGLAKPVFLAFSKFGKGLTPLDTYLGQVSVENFLNSVGQGFAS", "aa_hexdigest": "bf903f80e1dca179388b132ae892eb90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.034700000002, "isoelectric_point": 8.189692878723147 }, "id": "EFPKPENHBP_91", "locus": "GCA000186005_00050" }, { "type": "cds", "sequence": "contig_1", "start": 27368, "stop": 27514, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGGACACGTTATGTTCCAACAAGCAGCTGTTGTTGTTCCAGTTGTACTTGTATTAATGTTCTCAGGACAACAATTTAGAACAGCAGAAGGTGCTATTAATGTAGGCGGCCAAGTCGGTACAATCTTCTTCTCAGGTTGA", "aa": "MITGHVMFQQAAVVVPVVLVLMFSGQQFRTAEGAINVGGQVGTIFFSG", "aa_hexdigest": "59b89b24fbb2d274c7f0eb89921fdb37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5040.879099999999, "isoelectric_point": 6.501948738098144 }, "id": "EFPKPENHBP_92", "locus": "GCA000186005_00051" }, { "type": "cds", "sequence": "contig_1", "start": 27611, "stop": 27853, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ], "nt": "ATGTTCGGTATTGCTATTGCATACCAAATTGGTAAATTCTTCGGTAAAAAAGAAGATTCAGCAGAAACTAAAAAACTTTCAAACAAAGTTAAATTATTAGAAGACAATATCTTCACACAAAGTGTATTGATCTTATTAATTTTCTTAATCCTTATCTTAATTATTCAATTCGCACCTGCAGGTGCATCAGTAAGATTTGGTTTAAGCAATGGACTTGTAAACAAAGCAATTTATGGTTCATGA", "aa": "MFGIAIAYQIGKFFGKKEDSAETKKLSNKVKLLEDNIFTQSVLILLIFLILILIIQFAPAGASVRFGLSNGLVNKAIYGS", "aa_hexdigest": "febae287c0e4d5a8ae6e901578a2b714", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B4D71", "query_cov": 0.8125, "subject_cov": 0.9848484848484849, "identity": 0.585, "score": 75.1, "evalue": 2.35e-16, "valid": false, "product": "PTS transporter subunit IIC", "uniref50_id": "UniRef50_A0AAX3F0H9" }, "pscc": { "uniref50_id": "UniRef50_A0AAX3F0H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8750.335599999991, "isoelectric_point": 9.631657218933103 }, "id": "EFPKPENHBP_93", "locus": "GCA000186005_00052" }, { "type": "cds", "sequence": "contig_1", "start": 27932, "stop": 28555, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCTGGTATTCGTATGTTCGTTTCAGAACTTCAACAAGCTTTTCAAGGAATTAGTGAAAAATTAGTTCCAGGATCAGTTGTTGCTGTTGACGTTGCTGCCACATATGGTTTCAGCCAAAACTCAGTTACCTATGGTTTCTTATTTGGTTCAATTGGCCAATTCTTAGCTATGGGTCTTGTAATTGGATTAGCTCAAATTCCAAACTCAAGATTTGAAATTGTTATGGTAGTTCCATTATTCATTACATTGTTCTTTAACTCAGGTTCAATTGGAGTATTTGCAAATGCTTCAGGTGGATATAAAGCTACTATTGCAGTACCAATGATCTTTGGTTTCGTTGAAATTATTGTTGTTGCTCTTGCTTTAAGTATGATTAAAAACTTTGGTGTAATGCACCAAGTAGATCTTGACTTCCAATACTTTGAAAAAACTCTCACAAAAGCAGATGCATTCAAATCATTTACAGATTCATTAAATGCAAATTCTGCTAACTTGTTTATTCAAGGTGATGCTACTAAAGCTGTTTCAGCAGCACAATTAAATGATGCTATTACAAACATTAACAGTCAAGATTCATTTAAAATCTTAAAAAGCTTAAGTGAAGTTACAACAAACTGA", "aa": "MQAGIRMFVSELQQAFQGISEKLVPGSVVAVDVAATYGFSQNSVTYGFLFGSIGQFLAMGLVIGLAQIPNSRFEIVMVVPLFITLFFNSGSIGVFANASGGYKATIAVPMIFGFVEIIVVALALSMIKNFGVMHQVDLDFQYFEKTLTKADAFKSFTDSLNANSANLFIQGDATKAVSAAQLNDAITNINSQDSFKILKSLSEVTTN", "aa_hexdigest": "658b8435876284eae9cab90838aaf212", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22199.367700000003, "isoelectric_point": 5.01293773651123 }, "id": "EFPKPENHBP_94", "locus": "GCA000186005_00053" }, { "type": "cds", "sequence": "contig_1", "start": 28601, "stop": 28726, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATACTTATCAAGATATGTTTCAGACCAAATAGCTAAAGCTAAAGATATGAAAGAAATCTTGAAATTATCTTCATTCCAAAGAACACCATTCTTAACAGGATTTAATGGTATGTTTGACTGA", "aa": "MQYLSRYVSDQIAKAKDMKEILKLSSFQRTPFLTGFNGMFD", "aa_hexdigest": "8a8e49f17da4a40e9d73196cca569844", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4806.539099999999, "isoelectric_point": 9.301579093933103 }, "id": "EFPKPENHBP_95", "locus": "GCA000186005_00054" }, { "type": "cds", "sequence": "contig_1", "start": 28811, "stop": 28984, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGCTGCTCAATTCTTTGACTCACACAGACAACACAAACCTACAGTATTCCAAAGAATGCTTAAAGTTAAAGTCGAAACTTTAGAAGATCAAAAGAAAGCTGAAGAAGTTGCTAATGCACCAGTTGAAGAAGTTCCTCAACCAGAAGTTGCAGAACAAGCTGCTTAA", "aa": "MIFAAQFFDSHRQHKPTVFQRMLKVKVETLEDQKKAEEVANAPVEEVPQPEVAEQAA", "aa_hexdigest": "9ef2f2516a6357660ae7dc9281ae3a0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6522.337500000003, "isoelectric_point": 5.218922233581542 }, "id": "EFPKPENHBP_96", "locus": "GCA000186005_00055" }, { "type": "cds", "sequence": "contig_1", "start": 29083, "stop": 29361, "strand": "+", "frame": 1, "gene": null, "product": "PTS EIIB type-2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_041594238.1", "SO:0001217", "UniParc:UPI0002EBAD74", "UniRef:UniRef100_C4XFJ9", "UniRef:UniRef50_A0A0D5ZIZ4", "UniRef:UniRef90_C4XFJ9" ], "nt": "ATGAAAGTATTATGTGTTTGTGGCTCAGGTATGGGCACAAGTATGATTATTAAAATGAAAGTTCAACAAGCTATGAAAGAACTTTCAATTCCAGGAACAGTTGAAGCTTTAGGTTTAGGTCAAGGCAAAACAGTTGTAAACAACTTTGATGTTGTTTTGTGTACCTCAAACTTTGTTTCAGAAGTATCAAAAGGCAAAGCAAAAGCTTATGGTTTAAAAAACATCATGGATGTTAATGAAATCAAAGCTGCTCTAAAAGATGCATCAAGCAAGATGTAA", "aa": "MKVLCVCGSGMGTSMIIKMKVQQAMKELSIPGTVEALGLGQGKTVVNNFDVVLCTSNFVSEVSKGKAKAYGLKNIMDVNEIKAALKDASSKM", "aa_hexdigest": "a8172f4c7a6c8a860341a396a2478c4b", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002EBAD74", "ncbi_nrp_id": "WP_041594238.1", "uniref100_id": "UniRef100_C4XFJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002EBAD74", "RefSeq:WP_041594238.1", "UniRef:UniRef100_C4XFJ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFJ9", "uniref90_id": "UniRef90_C4XFJ9" }, "psc": { "uniref90_id": "UniRef90_C4XFJ9", "product": "PTS EIIB type-2 domain-containing protein", "uniref50_id": "UniRef50_A0A0D5ZIZ4" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZIZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZIZ4" ], "product": "PTS sucrose transporter subunit IIABC" }, "genes": [], "id": "EFPKPENHBP_97", "locus": "GCA000186005_00056" }, { "type": "cds", "sequence": "contig_1", "start": 29571, "stop": 29849, "strand": "+", "frame": 3, "gene": null, "product": "PTS sugar transporter subunit IIA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "nt": "ATGCCACATGCTTCAGCAACAAAAGACGCTGTTTTTGGAAACGGATTCTCACTTATTACTCTTAAAAAACCTATCAAATTTGAAGGAGATGAAAGAGAAGTAAAAATCCTTATGGGACTAGCCGCAAAAGATGGTGAAACTCATACATCTGTTGCTATTCCACAAATTATTGCTGTTTTTGAAGATCCACAAAATATTGAAAAAATAGCACAAGCTAAAACAAAAGAAGAAATTATAAATATCATAAAAGGTGTTGATTATACTAAATACCTTAGTTAG", "aa": "MPHASATKDAVFGNGFSLITLKKPIKFEGDEREVKILMGLAAKDGETHTSVAIPQIIAVFEDPQNIEKIAQAKTKEEIINIIKGVDYTKYLS", "aa_hexdigest": "78542f5ce103e681644f869f4883fd5a", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFC0FB", "query_cov": 0.967391304347826, "subject_cov": 0.8557692307692307, "identity": 0.596, "score": 103.0, "evalue": 8.23e-27, "valid": false, "product": "PTS sugar transporter subunit IIA", "uniref50_id": "UniRef50_UPI002FEFC0FB" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEFC0FB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "product": "PTS sugar transporter subunit IIA" }, "genes": [], "id": "EFPKPENHBP_98", "locus": "GCA000186005_00057" }, { "type": "cds", "sequence": "contig_1", "start": 29858, "stop": 30217, "strand": "+", "frame": 2, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGAAACCAATGTTACAAATTGCTTTAGATAACTTAACAATTGAAGATGCAATTGCAAGTGCCAAAAAGGTTGAAAAATACATAGACATTATTGAAGTAGGAACTATTCTTATTGCTTCAGAAGGTAAAAAAGCTATTAAAGCTTTGAGAGAAGCATTTCCTACAAAAATCATTGTTGCTGATGGCAAAATTGCAGATGCTGGGAAAGTTTTTGGAAAAATGTTTTTCGAAAATGGTGCTGACTTTACAACATGCATTTGTGCAGCTGAAGTGCCTACAATTGTTGAAACAATGAATATAGCCAAAGAATATGGCAAAAATAAAGAAGTTCAAATTGAATTAACTTCAAACTTTACTTGA", "aa": "MKPMLQIALDNLTIEDAIASAKKVEKYIDIIEVGTILIASEGKKAIKALREAFPTKIIVADGKIADAGKVFGKMFFENGADFTTCICAAEVPTIVETMNIAKEYGKNKEVQIELTSNFT", "aa_hexdigest": "c95f416a38be741e8cd606a348554817", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.865546218487395, "subject_cov": 1.0, "identity": 0.728, "score": 146.0, "evalue": 1.45e-43, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "EFPKPENHBP_99", "locus": "GCA000186005_00058" }, { "type": "cds", "sequence": "contig_1", "start": 30492, "stop": 30656, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAAAATAAAAAAAGACTTCTAGGCATCTATGAAAAAGCTATAAACAACAAATTTAGCTTAGAAGAAAAAATTATAATTGCTAAAGAAGCTGGTTATGACTTTATGGAATTTTCAGTTGATGAAAGTCCTCAAAGATTAGCTCGCTTAGACTGA", "aa": "MPNIENKKRLLGIYEKAINNKFSLEEKIIIAKEAGYDFMEFSVDESPQRLARLD", "aa_hexdigest": "89188bb3d15a1fee9f6ef502f4c23430", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.206900000001, "isoelectric_point": 5.368465614318848 }, "id": "EFPKPENHBP_100", "locus": "GCA000186005_00059" }, { "type": "cds", "sequence": "contig_1", "start": 30720, "stop": 30920, "strand": "+", "frame": 3, "gene": null, "product": "Sugar phosphate isomerase/epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "nt": "ATGACCCTTAGTGGTCATCGTAAATATCCTTTTGGTTCTAAAGATCCTAAAATCAGAGCTAGAGCCAAAGAAATAATGACTAAAGCAATTATTTTAGCCAAAAAATTAGGTATTAGAACTGTTCAACTTGCTGGTTATGATGTTTATTATGAACCAGGTGATGAAGAAACAAGAAAAAACTTTATTGAAGGAATGAAATGA", "aa": "MTLSGHRKYPFGSKDPKIRARAKEIMTKAIILAKKLGIRTVQLAGYDVYYEPGDEETRKNFIEGMK", "aa_hexdigest": "2f1942fdec59377ad574e7a8d649bca4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A936AG02", "query_cov": 1.0, "subject_cov": 0.8048780487804879, "identity": 0.606, "score": 85.9, "evalue": 1.11e-20, "valid": false, "product": "Sugar phosphate isomerase/epimerase", "uniref50_id": "UniRef50_A0A936AG02" }, "pscc": { "uniref50_id": "UniRef50_A0A936AG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "product": "Sugar phosphate isomerase/epimerase" }, "genes": [], "id": "EFPKPENHBP_101", "locus": "GCA000186005_00060" }, { "type": "cds", "sequence": "contig_1", "start": 30951, "stop": 31076, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTTGAAATTATGGATACTCAATTCATGGGAACTAATTCAAGAGCAATGACTTATATCAACATGATTAATAGTCCATATTTACAAATCTATCCTGATTTAGGTAATATTTATCAATGA", "aa": "MLAFEIMDTQFMGTNSRAMTYINMINSPYLQIYPDLGNIYQ", "aa_hexdigest": "78a88673ce127e65dc7852d2fbfdc0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.524100000001, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_102", "locus": "GCA000186005_00061" }, { "type": "cds", "sequence": "contig_1", "start": 31092, "stop": 31265, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGTATAACGAATTTGAACTTGCTAGAAATCATATAGTAGCTTTCCACTTTAAAGATACTGTACCTGGTAAATTTAGAGATACACCTTTTGGAACTGGCACTGTAGATTTCCCTTATCTTTTAAAAATATTAAAAGATTTAAAAATGAACCAACCTATTATGATAGAAATGTGA", "aa": "MYNEFELARNHIVAFHFKDTVPGKFRDTPFGTGTVDFPYLLKILKDLKMNQPIMIEM", "aa_hexdigest": "4ec6181250e55cf9a0c8a574a353f138", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 58, "aa_cov": 0.9473684210526315, "hmm_cov": 1.0, "evalue": 1.443977300619407e-10, "score": 37.551326751708984, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 6719.8261, "isoelectric_point": 6.707649040222168 }, "id": "EFPKPENHBP_103", "locus": "GCA000186005_00062" }, { "type": "cds", "sequence": "contig_1", "start": 31370, "stop": 31474, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATAAAAAATATGAATCTGAAATCAAAAAACTTAAACAAGAAGTTTATGAAGCTAATATGTTATTAGTTAAATATGGACTTGTTATTCATACTTGA", "aa": "MAIDKKYESEIKKLKQEVYEANMLLVKYGLVIHT", "aa_hexdigest": "5dc66d56658d75c69e823906de281374", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4026.7597000000005, "isoelectric_point": 8.029940223693849 }, "id": "EFPKPENHBP_104", "locus": "GCA000186005_00063" }, { "type": "cds", "sequence": "contig_1", "start": 31511, "stop": 31873, "strand": "+", "frame": 2, "gene": null, "product": "L-ribulose-5-phosphate 4-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "nt": "ATGGTTATTAAACCAAGTGGTGTTTCTTATGACACACTTAAAGCTGATGACATGGTCATAACTGATTTAGATAACAATGTATTTGATTCTAAATATCGCCCTTCTTCAGACACACCTACTCATACCTTACTTTATAAAGAAAATCCTGAAATAAAAGGTATTGTTCATACTCACAGTCCTCATGCAGTAGCTTTTGCACAAGCAGGTAAAGATATTCCTTGCTTTGGTACAACACATGCAGATAATTTTTATGGACCAGTGCCATGTGCTAGAGCTCTAACTCCAAAAGAAATTGATGGAGAATATGAACACAATACAGGACTTGTAATAATAGAAACATTTAAAAAACGCAAGATTGACTGA", "aa": "MVIKPSGVSYDTLKADDMVITDLDNNVFDSKYRPSSDTPTHTLLYKENPEIKGIVHTHSPHAVAFAQAGKDIPCFGTTHADNFYGPVPCARALTPKEIDGEYEHNTGLVIIETFKKRKID", "aa_hexdigest": "bb6b4f2a7f0b646380327b2d7ceac778", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6LVT1", "query_cov": 0.8416666666666667, "subject_cov": 0.9901960784313726, "identity": 0.594, "score": 128.0, "evalue": 2.97e-36, "valid": false, "product": "L-ribulose-5-phosphate 4-epimerase", "uniref50_id": "UniRef50_R6LVT1" }, "pscc": { "uniref50_id": "UniRef50_R6LVT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "product": "L-ribulose-5-phosphate 4-epimerase" }, "genes": [], "id": "EFPKPENHBP_105", "locus": "GCA000186005_00064" }, { "type": "cds", "sequence": "contig_1", "start": 31982, "stop": 32101, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTAATACCTTAATAGTTTCAAATAATAGTGCTCCAACTGCGCAAGAAGCATTGATTGAAAAGCACTATAACCGTAAGCACGGAAAGAATGCTTATTATGGTCAAAATAAATAA", "aa": "MAINTLIVSNNSAPTAQEALIEKHYNRKHGKNAYYGQNK", "aa_hexdigest": "0f6872b9d7517e1b12f6900b7304aad9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4388.875100000002, "isoelectric_point": 9.60174388885498 }, "id": "EFPKPENHBP_106", "locus": "GCA000186005_00065" }, { "type": "cds", "sequence": "contig_1", "start": 32076, "stop": 32462, "strand": "+", "frame": 3, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1", "UniRef:UniRef90_C4XFJ4" ], "nt": "ATGCTTATTATGGTCAAAATAAATAATATAGACTACTTCATTTTTGATTTAGATGGTACACTCTATGATGATAATAAAAACATCTTAGAATCAACCAAAAACACTTTAAAACAGCTTCAAAAGGAAAATAAAAATATAATAATTGCTACTGGTAGACCTTATTATATGAACTATAAATTAATTAAGGATTTAGATATTAAACTACCAATAATAGCAGTTAATGGGGCTATGATTTATGATCCTATCACTAAAGAAGTGCCTTATTTTCAAGGACTAGACAAAAAAGTTGTTCAAGAATTAATTAACTTTTTAGAAGAGAATAGAATTGATTTTTTAGCTTACGATATTAATGGTATGAAAGGTAATAATTTCTCGAACCCTTATTGA", "aa": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPY", "aa_hexdigest": "7c82ae5ddbbb9a93d9a8c8ed992b41db", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 32076, "stop": 32765, "observations": { "mutation": [ 32460, 32511 ], "stop": [ 32460, 32511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ4", "query_cov": 1.0, "subject_cov": 0.44912280701754387, "identity": 1.0, "score": 257.0, "evalue": 1.08e-84, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPYWFEFMIYKNVVETNPYRWDYQEGSLKETCNQFNFAKILIITQNVPQEKIDSLLTIASKFPKQIYTVSSQKGVIDIMPIGISKGETLKKIAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND" }, "paralog": false, "identity": 0.9912663755458515, "score": 449.0, "evalue": 1.21e-162, "description": "Internal stop codon at 32460, 32511. Nonsense mutation around 32460, 32511.", "psc": { "uniref90_id": "UniRef90_C4XFJ4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ4", "UniRef:UniRef50_A0A3P8MEN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1" ], "product": "Cof-like hydrolase" } }, "id": "EFPKPENHBP_107", "locus": "GCA000186005_00066" }, { "type": "cds", "sequence": "contig_1", "start": 32685, "stop": 32933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCCAATAGGAATATCAAAAGGTGAAACATTAAAAAAAATAGCTAAGATTAAAAATATTGATTTATCAAAAGCAATGGCTTTTGGTGATGCTGATAATGACATAAGTATGTTAAAATCTGTTAAAATTTCAGTTGCAATGGGTAATGCAACAGAGAATTTAAAGAAAGTTGCAACTTATGTAACAGATACAAATAATAATGATGGAATTGCCAAAATTATTAAAGAATTAGAGTTAGTAAATGACTAA", "aa": "MPIGISKGETLKKIAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND", "aa_hexdigest": "3df4e08f2e3fe2a5236892bf55f0f750", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 74, "aa_cov": 0.9024390243902439, "hmm_cov": 1.0, "evalue": 3.901089363556826e-30, "score": 101.71113586425781, "start": 1, "stop": 74 } ], "seq_stats": { "molecular_weight": 8824.179999999997, "isoelectric_point": 7.9259527206420906 }, "id": "EFPKPENHBP_108", "locus": "GCA000186005_00067" }, { "type": "cds", "sequence": "contig_1", "start": 33049, "stop": 33363, "strand": "+", "frame": 1, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3", "UniRef:UniRef50_UPI001F306D39", "UniRef:UniRef90_C4XFJ3" ], "nt": "ATGAGACATTTAGAAATTAAATATAATCCAGGGTATTTTAATTTCAATGGTGATCTAAGATTTAAAGCATTCAATGAGTTACAACGAGTACATAAAGTAGGTATCTCAATTAATCCTTCAGCGGTTAAAGATAAAGACAATTATTTCAAGTTTCATATTAGTGAAAATGTTGAAGTAGTGTTAGATGATTTATCACTAAAATTAATTAGTAAAGGTAGATCGTTTGCCTGTCCAATGCATTTCTTTGATGATTTATACTTAGAATATTTTGATGAGAAAAAAGTAACTAAAAATCAAAAATTGAGATTAACTTGA", "aa": "MRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKDKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLT", "aa_hexdigest": "42ee6cf8daede0351ca1ce8cbe4c8d1a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F306D39", "query_cov": 0.8269230769230769, "subject_cov": 0.8269230769230769, "identity": 0.7559999999999999, "score": 140.0, "evalue": 1.59e-41, "valid": false, "uniref50_id": "UniRef50_UPI001F306D39" }, "pscc": { "uniref50_id": "UniRef50_UPI001F306D39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F306D39" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 32926, "stop": 33414, "observations": { "mutation": [ 32974, 33013, 33361 ], "stop": [ 32974, 33013, 33361 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ3", "query_cov": 1.0, "subject_cov": 0.6419753086419753, "identity": 1.0, "score": 218.0, "evalue": 2.36e-71, "gene_start": 1, "gene_end": 104, "reference_start": 42, "reference_end": 145, "reference_sequence": "MTKIKVYKVNIIENRYWYCPSLFTFSRRLWASRPFATLEELMRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKDKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLTWRKYFFNIEVVEKEQTE" }, "paralog": false, "identity": 0.9814814814814815, "score": 320.0, "evalue": 5.71e-114, "description": "Internal stop codon at 32974, 33013, 33361. Nonsense mutation around 32974, 33013, 33361.", "psc": { "uniref90_id": "UniRef90_C4XFJ3", "product": "MPN499 family protein", "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ3", "UniRef:UniRef50_C4XFJ3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3" ] } }, "id": "EFPKPENHBP_109", "locus": "GCA000186005_00068" }, { "type": "cds", "sequence": "contig_1", "start": 33624, "stop": 33914, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_110", "locus": "GCA000186005_00069" }, { "type": "cds", "sequence": "contig_1", "start": 33980, "stop": 34537, "strand": "+", "frame": 2, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_111", "locus": "GCA000186005_00070" }, { "type": "cds", "sequence": "contig_1", "start": 34937, "stop": 35341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGATTAAATTAGGTGATGGTGTTTTTTATGGTTGTAGAAGTCTAACAAAAATTATCTTCAACAGTCAACTTCAAATAATTGGAAAAGAAACTTTTAGCCATTGTAATTCAATAGAAGAAATAAACATTCCAGATACTGTAAATTTAAACACTGAAGGTGCTTTTGAAATGTGTTTTAATTTAAAAACCGTTCATATCCCAAATAACACAACAATAATTAAAACTAATGCCTTTAAACAGTGCGAATCATTAGAAACATTAGACTTAACAAATACCAACGTGAATCAAATACAAGCAAATACATTTAAAGGATGTACAAACCTAAAAGAAGTTAAGGTCAAAAAAGACACTCAAATAGACCCTCAAGCAAGCGATAATAATTCAATTTTTGTTTTTAATATTTAA", "aa": "MIKLGDGVFYGCRSLTKIIFNSQLQIIGKETFSHCNSIEEINIPDTVNLNTEGAFEMCFNLKTVHIPNNTTIIKTNAFKQCESLETLDLTNTNVNQIQANTFKGCTNLKEVKVKKDTQIDPQASDNNSIFVFNI", "aa_hexdigest": "c764be6894559c8a073df3ec6f6d9980", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 125, "aa_cov": 0.9253731343283582, "hmm_cov": 0.968, "evalue": 3.8520570475003846e-29, "score": 97.4892349243164, "start": 3, "stop": 126 }, { "id": "PF13855.12", "name": "LRR_8", "length": 51, "aa_cov": 0.373134328358209, "hmm_cov": 1.0, "evalue": 3.554768969683782e-13, "score": 45.46135711669922, "start": 61, "stop": 110 } ], "seq_stats": { "molecular_weight": 15036.963899999975, "isoelectric_point": 5.628674507141112 }, "id": "EFPKPENHBP_112", "locus": "GCA000186005_00071" }, { "type": "cds", "sequence": "contig_1", "start": 35755, "stop": 35913, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTACAGTAGATGTTGAATACTTATCAAACCAAAATAAAGTAAGTGATGTTATCGTTGTAATTCTTAATGAATCAGAACACAAACACATTATTGATACAGCTCTTGGCGAAAAAATGACTAAACGTGCTGACAAATTAATGCTTGTTAAATAA", "aa": "MLCTVDVEYLSNQNKVSDVIVVILNESEHKHIIDTALGEKMTKRADKLMLVK", "aa_hexdigest": "0245acafaf42e63c52f2da9e2ed48dbf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5913.903799999999, "isoelectric_point": 6.024785423278809 }, "id": "EFPKPENHBP_113", "locus": "GCA000186005_00072" }, { "type": "cds", "sequence": "contig_1", "start": 36247, "stop": 36438, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAACATTAGAAAAATTTTAGCTTCAGGTGAATTAGATGACATTGTTGTTGAAGCAAAAAGCGAACCAACACAACCAGTTGTAGTAGTTGAAGCTCCAACACAAGAACAACCTACTGTTCAAGAAACCCCAGCAGTTACACCTGAACCTCAAGTTGAAACATCATCAATGACAGAAACTAAATGA", "aa": "MNANIRKILASGELDDIVVEAKSEPTQPVVVVEAPTQEQPTVQETPAVTPEPQVETSSMTETK", "aa_hexdigest": "88e98fbed809d246d02c73f7a196e5b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6789.516500000002, "isoelectric_point": 4.16302433013916 }, "id": "EFPKPENHBP_114", "locus": "GCA000186005_00073" }, { "type": "cds", "sequence": "contig_1", "start": 36487, "stop": 36753, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGGCAACCCCAGGTCGTGGTGAAACAGTTAGATTATTCACCAAAAAAGACTTCCAAGCTAAACAAGATGGAAATGATGAAAATTCTTCAAGTAAAGATACTTCAGCAAGAAACGCAAGAGAATATGCAATGATTCTTGCTTTAGGCGGTCCAAAAAACATCGTTAACACAAATAACTGTGCTTCAAGATTAAGATATGATATTAACGATAGAAACTTAGTAAATGAAGTTGAATGTAAGGCAGCAGGTGCAGTTGCACTTAAATGA", "aa": "MATPGRGETVRLFTKKDFQAKQDGNDENSSSKDTSARNAREYAMILALGGPKNIVNTNNCASRLRYDINDRNLVNEVECKAAGAVALK", "aa_hexdigest": "85e4901081aca55cc3e0ad07257329e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 31, "aa_cov": 0.3522727272727273, "hmm_cov": 1.0, "evalue": 2.1679909759023766e-12, "score": 42.57704544067383, "start": 45, "stop": 75 } ], "seq_stats": { "molecular_weight": 9604.646399999994, "isoelectric_point": 9.061563301086426 }, "id": "EFPKPENHBP_115", "locus": "GCA000186005_00074" }, { "type": "cds", "sequence": "contig_1", "start": 36979, "stop": 37161, "strand": "-", "frame": 1, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607", "UniRef:UniRef90_UPI002FF38607" ], "nt": "ATGCAAGGCAAATACTCATTCATGATTCTAGGATTACCGGGTGCAGCTGTTGCTATGATTTTAGCGGCTCCTAAAGAAAATAGAAAAGTGGCAGCTGGTACAGTTATTCCTGCAGCTCTTACTTCAGTTATTACTGGTGTTACAGAACCTATTGAATTTACATTCTTATTCTTAGCACCATGA", "aa": "MQGKYSFMILGLPGAAVAMILAAPKENRKVAAGTVIPAALTSVITGVTEPIEFTFLFLAP", "aa_hexdigest": "a333f9de734758970e8316a67670feb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 36931, "stop": 37179, "observations": { "mutation": [ 36981 ], "stop": [ 36981 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FF38607", "query_cov": 0.9833333333333333, "subject_cov": 0.7108433734939759, "identity": 0.831, "score": 98.2, "evalue": 1.18e-25, "gene_start": 2, "gene_end": 60, "reference_start": 8, "reference_end": 66, "reference_sequence": "MKIGRFADGKFSFMIFGLPAAAAAMILAAPKENRKLALGTVVPAAFTSIITGVTEPIEFTFLFLAPLLFWGFHALICIFIYVC" }, "paralog": false, "identity": 0.7439024390243902, "score": 130.0, "evalue": 4.87e-41, "description": "Internal stop codon at 36981. Nonsense mutation around 36981.", "psc": { "uniref90_id": "UniRef90_UPI002FF38607", "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FF38607", "UniRef:UniRef50_UPI002FF38607" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607" ], "product": "PTS transporter subunit EIIC" } }, "id": "EFPKPENHBP_116", "locus": "GCA000186005_00075" }, { "type": "cds", "sequence": "contig_1", "start": 37259, "stop": 37462, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAAATTTAATTTCATTACACCAATAGCAGCATATGAGTCACCCATTCATTCATTGTGTTTAGAAGCCATCAAAGCTTTTCAACCATTGTACGCAGTTAATGCAGTAGCATCTCCTGAAAAAGTATTTCCTCTTGCTTCTCAATCTTCAAGCGCTTTAAGAATATCACCGCCTGCATTTGTGTATCAAAGAGGTGCATAA", "aa": "MLKFNFITPIAAYESPIHSLCLEAIKAFQPLYAVNAVASPEKVFPLASQSSSALRISPPAFVYQRGA", "aa_hexdigest": "0effe25a92e7635d023a379d4464e728", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7269.399700000002, "isoelectric_point": 8.978850364685059 }, "id": "EFPKPENHBP_117", "locus": "GCA000186005_00076" }, { "type": "cds", "sequence": "contig_1", "start": 37588, "stop": 37827, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGGTAAATTTGGTGCTGAAGTTAAACAAGTCGTTGGTTCTTCATTAGGATTCAAAACTCTTCAAACTTCAGTTTTCGGTGGTATTTTTGTTGGTTTAATAGTTTCATACTTATACAACAAATTACACACAATTAAACTTCCAACTGTTATTTCATTCTTTGGAGGAAAACGTTTTGTTTCATTAATAACAATAGTTGCTATGATTCCATTAGCATTCTTATTCCTCCTTATATGA", "aa": "MTGKFGAEVKQVVGSSLGFKTLQTSVFGGIFVGLIVSYLYNKLHTIKLPTVISFFGGKRFVSLITIVAMIPLAFLFLLI", "aa_hexdigest": "6fb69cb74b120360696917bdbe8af925", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8585.338099999994, "isoelectric_point": 10.218255043029785 }, "id": "EFPKPENHBP_118", "locus": "GCA000186005_00077" }, { "type": "cds", "sequence": "contig_1", "start": 37828, "stop": 38355, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTACAGCACAAACTACAGGTATAGAAAACAAAAAATCTAAACCAAAAGACAATAGCCGTAAGGGAAATTTTCGTAAGATTCTAAGTAAAATCTCTGGTGCTTTCATGTTACCAATTTCCGTTATGTCTATAGCTGGTCTATTTCTAGGGGTTGGTGCAACAATATCAAGTCAATCAGCGATTGTTCTTAACAACAATCCATTATCATCTGCTGCTAAAGCTGGTATTGTAATTGGTGACTTTATTAAGTTATTAGGTGACCCAATATTTGGAGCAATGCCGCTATTGTTTGCCGCTGCCTTTGTTGTGGCATTTACCGACGAAGCCGGAGTCGGTGTCTTCGCCGCAATAGTTGGATACTTAGTATTCTCAGCAATACAAGCTCCATTTATTCAAAGTGTTATAGAAAAAACTAATATTGATGGCACTAAACAAGCTAGTGAATTAATAGGTAAAACAATACTTAAAGATGATAAAACAGGACAACTTTACTATGAAACATTAAAAGGATATAACATTCTTTGA", "aa": "MTTAQTTGIENKKSKPKDNSRKGNFRKILSKISGAFMLPISVMSIAGLFLGVGATISSQSAIVLNNNPLSSAAKAGIVIGDFIKLLGDPIFGAMPLLFAAAFVVAFTDEAGVGVFAAIVGYLVFSAIQAPFIQSVIEKTNIDGTKQASELIGKTILKDDKTGQLYYETLKGYNIL", "aa_hexdigest": "a215c937b1056fca8d3d7aade24d8e93", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18467.3631, "isoelectric_point": 9.42574520111084 }, "id": "EFPKPENHBP_119", "locus": "GCA000186005_00078" }, { "type": "cds", "sequence": "contig_1", "start": 39039, "stop": 39743, "strand": "+", "frame": 3, "gene": null, "product": "BPL/LPL catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "nt": "ATGAAAATTTTTAGAATTAAGGAAACTTCACCTTATGTTACTTTAGCTTTAGAAAATATTATTATGCATGATCATGATTTAAAAGGTGATGTACTTTTAATTTATCAACATGATAATGCTATTATTATTGGTAACAATCAAAATGCATATGAAGAAATTAACCGTTCTTTTGTAAAAGAAAATAATATTAAATTAGCTCGTAGAATGTCTGGCGGTGGAGCTGTGTATCATGATTTAGGCAATATTAATTTTAGTTTCATAACTGACTATGATAAAAAAGGCGGTTATGAAAGATTTCTAACTCCAATTATTGCTTTTCTTAAAAGCTTAGGTCTAGATGCTCAATTTAAAGGTCGCAATGATGTTGTGGTTAATGGCTGCAAAATAAGTGGTAACGCCCAATATATTAGTAATAATCGAATAGTAAGCCATGGAACATTATTATTTGATGTTGATTTAACAAAATTATCGAAAGCACTTAATCCTGCAAGAATTAAGTATGAATCTAAAGGCATTCAATCAGTTAGATCTAGAGTAGTTAATATTTATGATGAATTATTAAACAAAATGACAGCTGAAGAATTTATTAGTCGTCTAATTAATTATTTTGTTAAGAATTTTAAAGGAGAATTTATTGATATTGATTATGCAAAATATCAAGAACAATTAAACTCTTTACAACAAAAATTTGCTTCTGAAGAATGA", "aa": "MKIFRIKETSPYVTLALENIIMHDHDLKGDVLLIYQHDNAIIIGNNQNAYEEINRSFVKENNIKLARRMSGGGAVYHDLGNINFSFITDYDKKGGYERFLTPIIAFLKSLGLDAQFKGRNDVVVNGCKISGNAQYISNNRIVSHGTLLFDVDLTKLSKALNPARIKYESKGIQSVRSRVVNIYDELLNKMTAEEFISRLINYFVKNFKGEFIDIDYAKYQEQLNSLQQKFASEE", "aa_hexdigest": "15eb3472949c422187a5f6ed7da5e547", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00111975EB", "query_cov": 0.9658119658119658, "subject_cov": 0.9576271186440678, "identity": 0.6729999999999999, "score": 311.0, "evalue": 7.48e-105, "valid": false, "product": "lipoate--protein ligase", "uniref50_id": "UniRef50_A0A432FB30" }, "pscc": { "uniref50_id": "UniRef50_A0A432FB30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "product": "BPL/LPL catalytic domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_120", "locus": "GCA000186005_00079" }, { "type": "cds", "sequence": "contig_1", "start": 40032, "stop": 40520, "strand": "+", "frame": 3, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "ATGTATAAAAGACAATCAATTGAACTTTTAGGCGAAACAATTTATTATGAAAGAATAGATTGTTTTCAAAAAGAAAAAAGACCAGCAACTGTTTTACTTGTGCACGGTTTTAATTCTGATTCAAATACCTTTACTAGATTTATTGATTTTGCTGAAGTAGTGGCCGAAAATACACCCGATAATGACGAATTAAGAGAAGAATATAGAAACTTTCATCGTTTCAATTTATATGCTTTAGATTTGCCTGGTTGTGGAAAATCTGGAAAAAATAGTCAAATAACACTTGATTTATATAACAAAATAGTAGTTGAATTTATTAACACAGTTTTAAAAGACCACGAAGTAATTTTAATTTCGCATTCAATGGGTTCAGTACCTTGTTTGTATGCTAAAAAAGAATGCAAAAACGTAACTAGGTTATTTATGGTAGCTCCTTTAAATTATGCTTTAGTTGACGATAAGCAAAAACTAATTAATCTTAAAAAATGA", "aa": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKK", "aa_hexdigest": "d37823e7e29b412ec68943aaa9590935", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 40032, "stop": 40823, "observations": { "mutation": [ 40518 ], "stop": [ 40518 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.552901023890785, "identity": 1.0, "score": 331.0, "evalue": 5.17e-113, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.9961977186311787, "score": 532.0, "evalue": 7.9e-195, "description": "Internal stop codon at 40518. Nonsense mutation around 40518.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "EFPKPENHBP_121", "locus": "GCA000186005_00080" }, { "type": "cds", "sequence": "contig_1", "start": 40521, "stop": 40883, "strand": "+", "frame": 3, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "TTGATTCCTGCTAATGTTGAAGATGCTAGAAATTCATTAAAAGCATTGGTTTATAATCCTGAAGATTATTATTTAAGAAACTTGGATTATAATGCTGAAAAATTAGCGAGCAAATATAATGAAAACAAAGAAACTTTTTCTTATCTATTAAATGAACAAATTTTAAATAAAGATTATTTATTAAAACATGTAAAGCCTTTATTTGCAAATCCAAATGGTTGTGTAATTATTTATGGTGAAAATGATGAATTTGTGACACCAAAAGAAATTAAAAAAATAGAAGAAGATTATGGTTATAAAATTGTGCAATTAAATGAATGTGGACATGCTGCATTTTATCAAAAATTAAATACCATTTATTGA", "aa": "MIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIY", "aa_hexdigest": "30b36ee128c2e7586cf6ca2c83cdd545", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 40221, "stop": 40913, "observations": { "mutation": [ 40518, 40881 ], "stop": [ 40518, 40881 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.40955631399317405, "identity": 0.992, "score": 248.0, "evalue": 4.41e-81, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.991304347826087, "score": 462.0, "evalue": 5.56e-168, "description": "Internal stop codon at 40518, 40881. Nonsense mutation around 40518, 40881.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "EFPKPENHBP_122", "locus": "GCA000186005_00081" }, { "type": "cds", "sequence": "contig_1", "start": 41063, "stop": 41557, "strand": "+", "frame": 2, "gene": null, "product": "Glycosyltransferase 2-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6", "UniRef:UniRef90_C4XFI6" ], "nt": "ATGAATAGGCAAAAAACTTTATATCAACCTAAAATTTCAATTTTAATTCCCTTGCATAATAGTGCTAAATATTTTAATTCCACTATTAAAACTATTCTTAATCAAAGTTATGAAAATTTAGAAATTTTATTCCATGATGATGCCTCTAATGATGAAACAAGAAAAATCTTAGAAGATTTGACCATTAAAGATTCTAGAGTTAAATATATCTTTTCTGATACTAATATTGGTTCAGGATATTCAAGAAACAAATTAATTGAAGCTTCTACCGGAGATTTCATTTTTTTCATCGATGATGATGATGAATTTTTAAGTCCTAAAATAATAGAACGTTGTGTTAAATATTTAAAGCCTAATTTAGAAATACTTTCAACACAATTTAAATATAAATTTGATGATTTAGGTATTAAAATACCTATCAATAATATTGTTAAAACATTGAAAATCAATCCTTCACCAATGCATTATTATTTGAATACCACAACTTTTGCTTGA", "aa": "MNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFA", "aa_hexdigest": "819d4df325371f604d7a18fecc4fcf32", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "both", "pseudogene": { "start": 40991, "stop": 41860, "observations": { "mutation": [ 41015, 41555 ], "stop": [ 41015, 41555 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI6", "query_cov": 1.0, "subject_cov": 0.4568245125348189, "identity": 1.0, "score": 327.0, "evalue": 1.19e-110, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MFIFIDNYWMFSKISNIYYIKRKNMNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFAWGHFFNKKFLYDIDVNFSKVKKYEDIAEMGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDVSFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKIPKVKK" }, "paralog": false, "identity": 0.9896193771626297, "score": 574.0, "evalue": 2.44e-210, "description": "Internal stop codon at 41015, 41555. Nonsense mutation around 41015, 41555.", "psc": { "uniref90_id": "UniRef90_C4XFI6", "product": "Glycosyltransferase 2-like domain-containing protein", "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI6", "UniRef:UniRef50_C4XFI6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6" ], "product": "Glycosyltransferase 2-like domain-containing protein" } }, "id": "EFPKPENHBP_123", "locus": "GCA000186005_00082" }, { "type": "cds", "sequence": "contig_1", "start": 41639, "stop": 42070, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAACATTTTTTCCAAATGTAAGAATTTTAAAAGAACTTTGATTTATTCTATAATTTATCATAGAAAAACCAATTCATTAACTTCATTTGATAAAAACTATATCAATAAAATTGAATGTATCAACCAAGCCTACAAATTTAATTTAGAAAACATAGTATCAAATTTAAAAAATGATGTAAGTTTTAAAATTCTTAAAAAAACTGTAAATTCCTTATTTATGGAACATTTAAGTTTGATTAGTGTTTACTATATCAATTTAAAAAATCGAAATTTAAAAAAAGAATTTAAAAAATTCATCAAAGATATTTATCCTTCAATTTTAGAAATATATAATGGTTTTGATGTAAAAATCAAATATCGAAGAATTTCTGATTTACCAGTTTTTGTTACTAAATTTTTATATAAAATACCAAAAGTTAAAAAATAG", "aa": "MGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDVSFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKIPKVKK", "aa_hexdigest": "1d3a3c88af45e7ccdb2d5b35d0aa79f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17170.183899999996, "isoelectric_point": 9.956835746765137 }, "id": "EFPKPENHBP_124", "locus": "GCA000186005_00083" }, { "type": "cds", "sequence": "contig_1", "start": 42083, "stop": 43075, "strand": "+", "frame": 2, "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "db_xrefs": [ "EC:5.1.3.2", "GO:0003978", "GO:0006012", "RefSeq:WP_013526609.1", "SO:0001217", "UniParc:UPI0001F59A9D", "UniRef:UniRef100_C4XFI5", "UniRef:UniRef50_A0A507SK18", "UniRef:UniRef90_C4XFI5" ], "nt": "ATGACTTATTTATTAATTGGTGGAGCAGGTTATATCGGAAGCCATGTAGCTGAAGTTATAAATAAAGAAAATCAAAATAAAGTAATTATTTATGATAATCTTTCAACAGGATTTAAAGAATTTATTGAAGAAAAAAGTAAATTCATTCATGGAGATATTTTAGATATAAAAACATTAGATTTAACTTTTAAAAAAAATAAAATAGATGTAGTAATTTATTTAGCAGGTTTAATTAAAGTTGGTGAAAGTGTACAAAAACCACTTGACTATTATGAAACCAATATTCAAGGCTTAGTAAATGTTTTAAAAACAATGAAGAAGCATAATGTTAATAATTTGGTATTTTCTTCATCAGCAGCAGTATATGGTAATAACTCATTACACGATGGTTATTTTTTTGAAGAAGACCCAAAAGAACCATGTAGTCCTTATGGTAGAACCAAATATTTTGGAGAACAAATTATCAAAGACTTTGCTAATACAAATAGTGATTTCAAATATACTTTTTTAAGATACTTCAATGTTGCCGGTGCTTCAAAAAGTAAAAGAATTGGTTATTTAACCAAAAATGGTGTTGCACCGACTCATCTTATACCTGCTATTAGTTATTATGCTTTTGGTTTAATTCCCGAATTCAAAATTTTTGGTAATGACTATAACACACCTGATGGGACATGCATAAGAGATTATGTATATGTATATGAATTAGCAGAATTACATTTATTAACTGCAAAAAAAATGATTAAAGAAAAAACTTCATTATATTACAACATTGGTAGCTCAAAAGGCTTTTCAAATCTTGAAGTTGTAAATGCTTTTGAAAAAAATTTAAGCAAGAAATTAAATATTGAATATGGTCTAAGAAGAGCAGGAGACCCTGATAAATTAATAGCTTCAAATAAAAAAATATGTAAAGAATTAAATTATGAAGTAAAAACTAATTTAGATGAAATAATTTCTAGTGAGATTGAATTTAGAAAAGAACATTTATAA", "aa": "MTYLLIGGAGYIGSHVAEVINKENQNKVIIYDNLSTGFKEFIEEKSKFIHGDILDIKTLDLTFKKNKIDVVIYLAGLIKVGESVQKPLDYYETNIQGLVNVLKTMKKHNVNNLVFSSSAAVYGNNSLHDGYFFEEDPKEPCSPYGRTKYFGEQIIKDFANTNSDFKYTFLRYFNVAGASKSKRIGYLTKNGVAPTHLIPAISYYAFGLIPEFKIFGNDYNTPDGTCIRDYVYVYELAELHLLTAKKMIKEKTSLYYNIGSSKGFSNLEVVNAFEKNLSKKLNIEYGLRRAGDPDKLIASNKKICKELNYEVKTNLDEIISSEIEFRKEHL", "aa_hexdigest": "ee1e43407a438734a3d2e3aa37eace2f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F59A9D", "ncbi_nrp_id": "WP_013526609.1", "uniref100_id": "UniRef100_C4XFI5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59A9D", "RefSeq:WP_013526609.1", "UniRef:UniRef100_C4XFI5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFI5", "uniref90_id": "UniRef90_C4XFI5" }, "psc": { "uniref90_id": "UniRef90_C4XFI5", "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "ec_ids": [ "5.1.3.2" ], "uniref50_id": "UniRef50_A0A507SK18", "go_ids": [ "GO:0003978", "GO:0006012" ] }, "pscc": { "uniref50_id": "UniRef50_A0A507SK18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A507SK18" ], "product": "UDP-glucose 4-epimerase" }, "genes": [ "galE" ], "id": "EFPKPENHBP_125", "locus": "GCA000186005_00084" }, { "type": "cds", "sequence": "contig_1", "start": 43114, "stop": 43590, "strand": "+", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4", "UniRef:UniRef90_C4XFI4" ], "nt": "GTGAATCTCACTTTTTTTGTTTTGTCCTTGTATAATATAAATTGCATCAAAAGAATTAAAAATAAAAATAATGAATTTATAACTGAATATATACATGTTTTTGTCGAAAAATACGATCTTTTAACATTTATGAATTCGTTAAATAGAAATAAATTACATCCCAATAATGTAAGAATTGAAACAGTTATTAAAGCACTAGAAATGTTTAAAGAATTAAATAACTTTAAGAATATTCATAAATCTTTAAATTTAAAACGCGAAACATTTCAAAAACTATTTGATGACTTATCAGATGATAGAAAAATGATTTCCAAAACCGATAAATTTTTTAAAGGTTTGACGTGCCCACATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTTCAGTTTTAAATAAATACAGAAATTGTAGAAGAATAATAGTTAATCAAAAAATTAAAAATAAACACAAAGATTGA", "aa": "MNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMFKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKD", "aa_hexdigest": "bae4b8e243c6510ed992a4b617416322", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 43072, "stop": 43893, "observations": { "mutation": [ 43588 ], "stop": [ 43588 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI4", "query_cov": 1.0, "subject_cov": 0.4906832298136646, "identity": 0.9940000000000001, "score": 313.0, "evalue": 1.15e-105, "gene_start": 1, "gene_end": 158, "reference_start": 15, "reference_end": 172, "reference_sequence": "MKCVKVIKARPTILVNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMFKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKDWIKYINQNSNVYDRLCNENRKNSLRTFCQKSVRTLIDEAKEKCLKIKFPHFTTIYKNINILIFDINISILPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLMNRATRNFICDYANEMQIQLKKR" }, "paralog": false, "identity": 0.9926739926739927, "score": 549.0, "evalue": 5.55e-201, "description": "Internal stop codon at 43588. Nonsense mutation around 43588.", "psc": { "uniref90_id": "UniRef90_C4XFI4", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI4", "UniRef:UniRef50_C4XFI4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4" ], "product": "Transposase" } }, "id": "EFPKPENHBP_126", "locus": "GCA000186005_00085" }, { "type": "cds", "sequence": "contig_1", "start": 43795, "stop": 44040, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAAGTCTGTAGGAAAGGGAGAAAGGCATAAAAAAGACCCTTCAATTCCAAAGAAAAGAAGAGTCGGATTACATATAAGTTTAAGACCAGAAATTACAACAATGTTCGATTTTGAATTTGATACAGTACATGGCAAAAGCGATGATTTATATCATATTGCTGTTTTAATGAATAGAGCAACACGAAATTTTATATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAA", "aa": "MPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLMNRATRNFICDYANEMQIQLKKR", "aa_hexdigest": "bb5b4b39f4644efdc6d01c57d7d58757", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9499.922899999992, "isoelectric_point": 9.774712562561035 }, "id": "EFPKPENHBP_127", "locus": "GCA000186005_00086" }, { "type": "cds", "sequence": "contig_1", "start": 43995, "stop": 44255, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3", "UniRef:UniRef90_C4XFI3" ], "nt": "ATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAATACATATGATTGATGAACATGAATTAAATATTAATACTTTAACCATTGACAATGGTTCAGAAAATTACACTTTAGATCAGATCAACACTATTAAGCAAATATATCATTGCGATGCTTTCAATTCAAGTCAAAAAGGTAGAATTAAAAATTGTAATAAAATGATAAGAAAATATAGACCAAAATCAATTACTTTTGATAATTTAACACATGAATGA", "aa": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIKNCNKMIRKYRPKSITFDNLTHE", "aa_hexdigest": "032228743de9e81b7b36b2298fecea41", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 43995, "stop": 44402, "observations": { "mutation": [ 44253 ], "stop": [ 44253 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI3", "query_cov": 1.0, "subject_cov": 0.6370370370370371, "identity": 1.0, "score": 175.0, "evalue": 5.46e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIKNCNKMIRKYRPKSITFDNLTHEWIEIINEKINNFVRHEVDDFPFAMSANQFEKFIQENKHLYPSFYTNLYA" }, "paralog": false, "identity": 0.9925925925925926, "score": 278.0, "evalue": 6.2e-98, "description": "Internal stop codon at 44253. Nonsense mutation around 44253.", "psc": { "uniref90_id": "UniRef90_C4XFI3", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI3", "UniRef:UniRef50_C4XFI3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3" ], "product": "Transposase" } }, "id": "EFPKPENHBP_128", "locus": "GCA000186005_00087" }, { "type": "cds", "sequence": "contig_1", "start": 44919, "stop": 45482, "strand": "+", "frame": 3, "gene": null, "product": "Glycosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2", "UniRef:UniRef90_C4XFI2" ], "nt": "GTGAAATTTAAGACTCAAACTCAAAAGCTAAAATATGGTATAGTCATTTGTGCTAGAAATGAAGAAAAAGTTATTGCTGAGTTAGTAAAAAGCATAAAAAATTCTCAATATGATCAAGATAAACTCCAAACATTTGTGATAGCACATAATTGTACAGATAAAACAGCGGAAAAAGCAAGAAATGCTGGTGCTATTGTTTATGAATATTCTAATTCTAGCGAAAGAACTAAGGGTTATGCATTAAAAAAAATATTTGAATTCATAGAACAAGACTATGGTATACAAAGTTTTGATGGATATCATATATTTGATGCGGACAATATAGTTGATAGTCTATATTTTGAAAAAATGAATGATGCTTTTTTACACTATGATAAAAAAAATGTAATTACTTCATTTAGAAATACTAAAAATTTTGGTAAAAGCATTCAAACAATTAATTATGGACTTATGTTTATGTTCCATACAACCATTGAATCAAACGCTAGAATGAAGTTTAAGATGTCTTCAAAAATTTTGGGTTCTGGTTTTTTAATTAGCAATGAAATGGTTAAAGATGGTTGA", "aa": "MKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDG", "aa_hexdigest": "bd160d29b4c432524c9c95793c6169ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 44802, "stop": 45785, "observations": { "mutation": [ 44883, 45480, 45606, 45624, 45648 ], "stop": [ 44883, 45480, 45606, 45624, 45648 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI2", "query_cov": 1.0, "subject_cov": 0.43488372093023253, "identity": 0.995, "score": 367.0, "evalue": 7.11e-125, "gene_start": 1, "gene_end": 187, "reference_start": 40, "reference_end": 226, "reference_sequence": "MVMVYQNVVYIVMPIIVAIFLIFSFQYWFVLFFGLFAKPVKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDGWNITIPSDDSDFTVEQISEGKNVKYCDEAMFYDEQPTKFKDMWRQRLRWVFGNRIVWRKRFKSLIKNIFSSKKEDDKKISKSSSIDILFMISPISFIGFLILLLNILLVSLGPIFGANATQMWIDWAISFSISHAIIYLILFVIGIITYARGRKRVQRVPWWKMIISLFIWPLFLALYFPLQIVAIFKNINKFNWTQIEHNKNI" }, "paralog": false, "identity": 0.981651376146789, "score": 628.0, "evalue": 3.95e-230, "description": "Internal stop codon at 44883, 45480, 45606, 45624, 45648. Nonsense mutation around 44883, 45480, 45606, 45624, 45648.", "psc": { "uniref90_id": "UniRef90_C4XFI2", "product": "Glycosyltransferase", "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI2", "UniRef:UniRef50_C4XFI2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2" ], "product": "Glycosyltransferase" } }, "id": "EFPKPENHBP_129", "locus": "GCA000186005_00088" }, { "type": "cds", "sequence": "contig_1", "start": 45669, "stop": 45848, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAAAAAATATATTTTCATCTAAAAAAGAGGATGATAAAAAAATATCAAAGTCTTCTTCTATTGATATTTTATTTATGATATCACCAATTAGTTTTATAGGTTTTTTAATTTTACTTTTAAATATTTTGTTAGTTTCACTTGGACCTATTTTTGGAGCTAATGCTACTCAAATGTGA", "aa": "MIKNIFSSKKEDDKKISKSSSIDILFMISPISFIGFLILLLNILLVSLGPIFGANATQM", "aa_hexdigest": "bae7fff709340b2d86514a3b27dee090", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6502.784399999997, "isoelectric_point": 9.303642082214353 }, "id": "EFPKPENHBP_130", "locus": "GCA000186005_00089" }, { "type": "cds", "sequence": "contig_1", "start": 46126, "stop": 46437, "strand": "+", "frame": 1, "gene": "cofD", "product": "2-phospho-L-lactate transferase CofD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1", "UniRef:UniRef90_C4XFI1" ], "nt": "ATGAAAATAACTATTATTTCAGGTGGATCAGGTAATGATGCACTAGTAAAAGGATTAAAAGCTTTTTACAAAGATAAAAATTTAAGCATTAAAGTTATTGTAAATGCTTATGATAATGGAAAATCTACTGGTGTATGTAGAAAAGTTACTGATACTTTAGGAGTATCAGATATAAGAAAAAATCACTCAAGAATGTATGTGAGTGAATATGGCGATAAAGTGGATAAAAACCTTTTAGAGTTTTATGAAAAAAGATTTGATTTCACTAAAAATAAAGAAGAATTAGAAATTTCTAAACTTTTAGATAAATGA", "aa": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDK", "aa_hexdigest": "04bbf442d71f3436bf8ce0bed53fd935", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cofD" ], "truncated": "3-prime", "pseudogene": { "start": 46126, "stop": 46621, "observations": { "insertion": [ 46582 ], "mutation": [ 46435 ], "stop": [ 46435 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI1", "query_cov": 1.0, "subject_cov": 0.6280487804878049, "identity": 1.0, "score": 202.0, "evalue": 3.01e-65, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDKWNLKEYKKYVSNFFKNPKANEFNYKDFSVSNIVYSQMYKELGYEKTNKHFAIKWELMISLF" }, "paralog": false, "identity": 0.9939024390243902, "score": 323.0, "evalue": 5.08e-115, "description": "Internal stop codon at 46435. Frameshift due to insertion around 46582. Nonsense mutation around 46435.", "psc": { "uniref90_id": "UniRef90_C4XFI1", "product": "2-phospho-L-lactate transferase CofD", "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI1", "UniRef:UniRef50_C4XFI1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1" ] } }, "id": "EFPKPENHBP_131", "locus": "GCA000186005_00090" }, { "type": "cds", "sequence": "contig_1", "start": 46543, "stop": 46701, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAGAATTAGGTTATGAAAAAACAAATAAACATTTTTGCGATAAAATGGGAATTGATGATTTCGTTGTTTTAAATTCATTTGATAATATCTTTATCAAGGCATTAACGGAATCTGGAACAATAATAGATGATGAAGGAAAAACTGTCTTTTGA", "aa": "MYKELGYEKTNKHFCDKMGIDDFVVLNSFDNIFIKALTESGTIIDDEGKTVF", "aa_hexdigest": "7153c4a67b0057a76b70011783c211a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5981.733999999999, "isoelectric_point": 4.603071784973144 }, "id": "EFPKPENHBP_132", "locus": "GCA000186005_00091" }, { "type": "cds", "sequence": "contig_1", "start": 46966, "stop": 47148, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAACCGGTCTTGACTTATCAAATGTAAAAATTTTACTTAATAAAAGTGCAAAAGAAACCTTAAAATTAACCATACCAAACTATAATTTCATAGTCAAAGATATGGGAAATATTAAGGGTAAACAAGAACCAGAAAAAATGGTTAAATCTGTTATTGATACCTATTATGATAAATAA", "aa": "MERTGLDLSNVKILLNKSAKETLKLTIPNYNFIVKDMGNIKGKQEPEKMVKSVIDTYYDK", "aa_hexdigest": "af346afa7d4768c0f60280ef41288d9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6905.020500000004, "isoelectric_point": 9.260577201843262 }, "id": "EFPKPENHBP_133", "locus": "GCA000186005_00092" }, { "type": "cds", "sequence": "contig_1", "start": 47161, "stop": 47457, "strand": "+", "frame": 1, "gene": null, "product": "MobA-like NTP transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9", "UniRef:UniRef90_C4XFH9" ], "nt": "ATGAAATTCGGAATTATACTATCTGCTGGTAATCAAACTAGATTTAAAAGCGCAACTCCTAAAGCCTTGGTTAATGTAAATGGAAAAATTTTACTTGAGGAAAATATTTTGCGAATGTCAAAATATTGTGATCATATTTTTACGATATGTTCTTCCCAAAATGAGCATTTTTTTGAAAAATATAATCATATTGCTATCTCATCAGGTAAGGGTAGTGGAGATGCTGTACTAAAAGCATTATTGGAAATCAAACAAAAATATGGTTTTTCTTCAAATGATACTTGTTTTATATTATGA", "aa": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFIL", "aa_hexdigest": "e22e22e646e501c11daa6d2383647b4f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 47161, "stop": 47760, "observations": { "mutation": [ 47455 ], "stop": [ 47455 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH9", "query_cov": 1.0, "subject_cov": 0.4224137931034483, "identity": 1.0, "score": 199.0, "evalue": 7.13e-63, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFILWGDCLIEDLIYKKVVENYKKVGIVPCVFEEKPYVQIFQKQDNKIDVLFSKFGENASSGFHDLSIFYFNCEEILKYLLDFQNKIIGRDGNYVHKHGNEMEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK" }, "paralog": false, "identity": 0.9899497487437185, "score": 402.0, "evalue": 2.98e-145, "description": "Internal stop codon at 47455. Nonsense mutation around 47455.", "psc": { "uniref90_id": "UniRef90_C4XFH9", "product": "MobA-like NTP transferase domain-containing protein", "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH9", "UniRef:UniRef50_C4XFH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9" ], "product": "MobA-like NTP transferase domain-containing protein" } }, "id": "EFPKPENHBP_134", "locus": "GCA000186005_00093" }, { "type": "cds", "sequence": "contig_1", "start": 47710, "stop": 47859, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGAAATTATGTTCATAAACATGGAAATGAAATGGAATTTTTAGATGTTTTTAATGAAACAGATATAAAAGCTCAAATATTAGAAATCAAAAATTATCAAGATATGTCATTTAACACTATAGAACAATTAAAAAATATAAAATAG", "aa": "MDGNYVHKHGNEMEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK", "aa_hexdigest": "10adb88e727c7890ba3c200d5df5fb29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5847.5224, "isoelectric_point": 4.817638969421386 }, "id": "EFPKPENHBP_135", "locus": "GCA000186005_00094" }, { "type": "cds", "sequence": "contig_1", "start": 47910, "stop": 48356, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATCTTTTGTCCGCTAGGACAAATAAAAGTGAGGGAATTAACCCTCCCTAAAATATTTGGCAACTTGTCGCTCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCATACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MSLYLEIWNIACRYSFLIINIVIIFCPLGQIKVRELTLPKIFGNLSLFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "2f3c5b7ff591f6f7e321ae457d1cb3ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17417.73539999999, "isoelectric_point": 9.727328300476074 }, "id": "EFPKPENHBP_136", "locus": "GCA000186005_00095" }, { "type": "cds", "sequence": "contig_1", "start": 49330, "stop": 50076, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B41DE", "UniRef:UniRef100_C4XE52", "UniRef:UniRef50_C4XE52", "UniRef:UniRef90_C4XE52" ], "nt": "ATGTTTTGGTTTCTTATTGAAAGATTTTTATATATAATTACATTTATGATAAAAATAGTTAATTTATCAAAAAAAGATAAAAAAAATCGATTGATTTTAAACAACTTAAATTTCAATATTCATGAAAATGAATTGCATGTTTTTTTGGGCAAAAATGGTGCTGGAAAAACAGTTACTTTAGGAATAATTTGTGGATTAGATTCAGAATTTTCTGGAAAAATTTTAAAAGATAATGTAGATATTTACAAATCAAATAAAACTAATAATTATTTGTTTGTTCCAACAGATATTAGATTTCCAAATCATTTAAAAGTTATTGATTTAATTAAATTTTATGTGAATGAAATTTTTGAATTAGAAACAAATGAATTATTTATAAATGAAACATTAAAAGAATTCCATTTAGATGAAAGAAAGGACTATCTTCCTAAAAAATTATCTTCTGGAGAAAAAAGAAAATTAATGCTATTAATATCTAAATTAGTAAAACCAAAATATTTATTCTTAGATGAACCAGAAGCTAATCTAGATCCTTATTCAAGAAATGAATTATTTAAACTTTTGAATATGTTAAAAAAAGAAGGAACTTCAATTTTCGCATCATCTCACTTAATATCTGAAGAATTGCAAGATATAGATAGAATAACAATTTTGCATAACAAAACTATCGTTCTAAGTGAAAAAGTAAAATCAGCAAAAAATTTAAAAATTATTTTTGATAATTTTAAGAGAGGTGGTTATGACTAA", "aa": "MFWFLIERFLYIITFMIKIVNLSKKDKKNRLILNNLNFNIHENELHVFLGKNGAGKTVTLGIICGLDSEFSGKILKDNVDIYKSNKTNNYLFVPTDIRFPNHLKVIDLIKFYVNEIFELETNELFINETLKEFHLDERKDYLPKKLSSGEKRKLMLLISKLVKPKYLFLDEPEANLDPYSRNELFKLLNMLKKEGTSIFASSHLISEELQDIDRITILHNKTIVLSEKVKSAKNLKIIFDNFKRGGYD", "aa_hexdigest": "da481b283756ebdcdf5fc56b9c84cf08", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000B41DE", "uniref100_id": "UniRef100_C4XE52", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B41DE", "UniRef:UniRef100_C4XE52" ] }, "ips": { "uniref100_id": "UniRef100_C4XE52", "uniref90_id": "UniRef90_C4XE52" }, "psc": { "uniref90_id": "UniRef90_C4XE52", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE52" }, "pscc": { "uniref50_id": "UniRef50_C4XE52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE52" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_137", "locus": "GCA000186005_00096" }, { "type": "cds", "sequence": "contig_1", "start": 50216, "stop": 51043, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51", "UniRef:UniRef90_C4XE51" ], "nt": "ATGATTTTTCTATTGGATTTTATTATAGTTTGTGTGTCAACATTATTTATTCTGCATTTAACATTTGTCTCAAACCAAAAATCGATATATGAAAACACATTAAATTGTAGATATTTTAATAAACCTAAAATTGTTTTAGCTTGGTCAATATATTCATTTATTTTATTATCCGCATTTTCATTATTAAAGTCTTTTGCAATAAGTTCATTGCATTTATATAGAGACACGAATATAAAAAGTTTTCTACTTTATTTTATAGGCTATTTTCTAAGTAATTTATTGATAATCATTTTTATTATTTTAGTTTTTGCAAGTTTTATATTTATAACAAAATCTTATAAAAAGTCTCTCGTCATTGCAACTTGTATATTACCTTTTTTTACTGTTGTGTCTTTATCATCAAAAACAATAGAAGCGCGAGCAAATAAAAAGGTAGAAATAAACAAGGATATAATAGTTTCTGGTGACAAGTATTACTTTTTATCAGATGATGAGATTAAAAGTAAATATAATTTGAACTTAATTTCATCTATATATTTTTTACCCAAAAATACTATAAATAAGTACTTTGATGAGAATTTCTTGACTTATAAAAATAATTCAGATACAAAAAATTATATATTCTCAACATTCAATTTGTCTATTCTAAAAAATAATAAAAAATTTGACAAAGATAAATTTAGAATTTATCATAACGATGGTGAGTTTTTTATAAAAATGAGTACAAATGAATTGGCTAATTATTTATATAGCAAATTAAAAAAATATTTTGAATCAGGTGGAGTTAATTTAGATAAATTACTTAAAAAAATAAAATCTAATATATGA", "aa": "MIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSGDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNI", "aa_hexdigest": "d0db43746aa6a7a40d47c6510953ecc0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 50069, "stop": 51346, "observations": { "mutation": [ 50120, 50213, 51041, 51134, 51149 ], "stop": [ 50120, 50213, 51041, 51134, 51149 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE51", "query_cov": 1.0, "subject_cov": 0.48672566371681414, "identity": 1.0, "score": 512.0, "evalue": 1.33e-178, "gene_start": 1, "gene_end": 275, "reference_start": 50, "reference_end": 324, "reference_sequence": "MTKKILSLTFKFNKKNKWVYIGLSLSFIISFIFCSLFLFDNYLFYNYFWMIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSGDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNIWIKNNFTGNEYNLVMHLLGKDDFNFQYLQRDWNDNLWNAAKLKSNLNSMFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSNSKITDSDLNFISEKMVNFVGDKVIIDFNKYAQSELENSITTTITKFNLYFPEIDSKEKWKNLVYSSSNHLFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE" }, "paralog": false, "identity": 0.9882352941176471, "score": 826.0, "evalue": 1.07e-304, "description": "Internal stop codon at 50120, 50213, 51041, 51134, 51149. Nonsense mutation around 50120, 50213, 51041, 51134, 51149.", "psc": { "uniref90_id": "UniRef90_C4XE51", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE51", "UniRef:UniRef50_C4XE51" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51" ], "product": "ABC transporter permease" } }, "id": "EFPKPENHBP_138", "locus": "GCA000186005_00097" }, { "type": "cds", "sequence": "contig_1", "start": 51185, "stop": 51496, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAATTAATTTCTTTGATTTTTTAAATCATTTATACAGATCAAATCAAACAAAATTTAACACCACAATTAACATGGCTGAATTTTATATTGTTAAAGATAATAATTTAATAAAATACTTAGATTCAAATAGTAAAATAACTGATTCAGATTTAAATTTTATTAGCGAAAAAATGGTGAATTTTGTTGGTGATAAAGTTATTATTGATTTTAATAAATATGCACAAAGTGAACTTGAAAATTCAATAACAACAACAATTACTAAATTCAATTTATATTTTCCTGAAATAGATTCAAAAGAAAAATGA", "aa": "MFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSNSKITDSDLNFISEKMVNFVGDKVIIDFNKYAQSELENSITTTITKFNLYFPEIDSKEK", "aa_hexdigest": "fa56cdd38d71c654c2de82f713b72066", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12219.709499999986, "isoelectric_point": 5.297644233703613 }, "id": "EFPKPENHBP_139", "locus": "GCA000186005_00098" }, { "type": "cds", "sequence": "contig_1", "start": 51527, "stop": 51766, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCATTATTAAACAAACAATTGATAAAATGAATGAACTTGTATCTAATTTAGGAAATTATCAAGCGTTTAAATTTGAAGAAAACGTAAATCCCAAAAACTTTTATGACAATACAAAAATAATAAAACTAAATAATTTGACATATGACCTGATAAACTTATTTTTAATAACATTTGTTATTTTAACACTCAGCATTTTGCCAATAATATTTACTTTTAAAAGGAGAAAACATGAATAA", "aa": "MFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE", "aa_hexdigest": "093021895d27c9b3ac1f070b46c997bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9458.04919999999, "isoelectric_point": 9.346964836120605 }, "id": "EFPKPENHBP_140", "locus": "GCA000186005_00099" }, { "type": "cds", "sequence": "contig_1", "start": 51759, "stop": 52721, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAGAAATTAAAAATTAATTTACTATCTATTTCAACAATAATGATGGGGGCATCATTTGTTGTCGCATCATCTTGTGGTTCTAAAGAACAAGAGCAAGAAAAAGTTTTAAGTGATAATGAATATGTTGAGAAACACTCTATAACAAGAGACACAAATATTAAGGAAACAAGATATATTAGTCAAAATGTAAACAAAGTTTTTGACAACCACATACAATTAGAAAATTTTGTTAAAAATAATATAAAATCTGTTGATTTGAAATTTTTAAAAAACTCAAAAAAATTTGATGATATAAATAAAAATAATCCACAATTTATGTCCAATGAAGAGCTTTCATATTTTGATTTTAGCAAGTCAACAAGTAACGAAAAAATGCCGATATTTTTAGGACGAAACGGGAAAGTTTTTACTTCATTAGAAAAAGCAAGAGATTCATATTTTAATATAAATCAAGTTTACAAATTAGGCAATATTTATGCAAACAGTAAAGAGGAACTTTACAATTTCATTTTAAATAAATCAGTAAATAATAAAGATTTTTTGATAGAAGTATTAAATGAAATAAAAAGCAAATTGAATTTTAATTCACCTAACGCAACTATTACAGAATTTTTCAAAAGTCCAAGCGGACAAATTACAAGTTTTAAAAAAGAAGACCTTAAAGATGCAGACAAAAAAAGCAGAATTGAAGATTACATTAAAAATGTTGCAAAGAAATATGTAATCTATTATGATGAAGAAAATAATCCTCACTTAAAAGCGGTTGAAGATTATATATTTGATAGAATATCAAACTCTGAATTTATTAAGAGTAAAATCACTAGAATTAATTCTAATAAAGGATATAACAAATATTATGTAGACAATTCCAAAGATGATGAATTTACTTTGTATGGGCCAAGAATTTTAGAGACTCCTTTTGAAAATATTGAAGAAATAACTGATACAACTAAATGA", "aa": "MNKKLKINLLSISTIMMGASFVVASSCGSKEQEQEKVLSDNEYVEKHSITRDTNIKETRYISQNVNKVFDNHIQLENFVKNNIKSVDLKFLKNSKKFDDINKNNPQFMSNEELSYFDFSKSTSNEKMPIFLGRNGKVFTSLEKARDSYFNINQVYKLGNIYANSKEELYNFILNKSVNNKDFLIEVLNEIKSKLNFNSPNATITEFFKSPSGQITSFKKEDLKDADKKSRIEDYIKNVAKKYVIYYDEENNPHLKAVEDYIFDRISNSEFIKSKITRINSNKGYNKYYVDNSKDDEFTLYGPRILETPFENIEEITDTTK", "aa_hexdigest": "6697426d5bd46219397135d13fa442ae", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37353.61180000001, "isoelectric_point": 8.22224941253662 }, "id": "EFPKPENHBP_141", "locus": "GCA000186005_00100" }, { "type": "cds", "sequence": "contig_1", "start": 52779, "stop": 53219, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAGTATTGTCACATCACTTTTCAAAATTGTGTATTTTAATGAAAACATAAAGAAAAATGTTGAAATTACACCATTTACACCTATAACAATAAATGTTGAAAAAGATCATTTAAACAATTTCAAAAAATGTACCAAAAACTTTTGTTTTGAAGGCACTAACCTATACGATTATTTATATAACAAATATGATCTTTTAGCAAAAGGAAAAGAATCTAATTTTCTTTCTAGTTTTTATATATTATATGTTGCGGGTTTAAACAAAATTATAGAATATAAAGCAAATCAAAAAGTGGTTGATGAATATAAAACATATTTTAAAATGATCATAAATGACATTAGCAAAATTTTTGCATCATTATTACCAAAATATTTCATTCATGAAGGAATTAGTGAATCAGAAAACTCAGATAATCAAGTAAATATATCAAAAATTTGA", "aa": "MNSIVTSLFKIVYFNENIKKNVEITPFTPITINVEKDHLNNFKKCTKNFCFEGTNLYDYLYNKYDLLAKGKESNFLSSFYILYVAGLNKIIEYKANQKVVDEYKTYFKMIINDISKIFASLLPKYFIHEGISESENSDNQVNISKI", "aa_hexdigest": "3e74e0684d3cb0cc6e916c9fa3a5ea68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17090.457899999998, "isoelectric_point": 8.372783088684084 }, "id": "EFPKPENHBP_142", "locus": "GCA000186005_00101" }, { "type": "cds", "sequence": "contig_1", "start": 53265, "stop": 53528, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCTAAATTATTGAAATATAAAATAGCATGCAATTTTTTGGATGTAATTGCAAGTGGCATATTTAATGCAGTATATAGATTTTCAAGTGAAGATTATGATCCAGAATCTCTCATTTTTCCTGAATATGCAATATATGATAATTATGAACATTATTTTGAAATTTATAGTTTAAAGCGATTTAAAAAACCAGTTTTTTCAAAAAATTATGACAAATTTACTAAACAATCATATGATACTAACAACAGCTTACAAGAATGA", "aa": "MIAKLLKYKIACNFLDVIASGIFNAVYRFSSEDYDPESLIFPEYAIYDNYEHYFEIYSLKRFKKPVFSKNYDKFTKQSYDTNNSLQE", "aa_hexdigest": "58bb8d6021ab725a8297f91dfee73712", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10441.674799999988, "isoelectric_point": 5.790495109558106 }, "id": "EFPKPENHBP_143", "locus": "GCA000186005_00102" }, { "type": "cds", "sequence": "contig_1", "start": 53544, "stop": 53642, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGACAGTAAAAAACAACTTTGATAATGTGAATGAATTAGTAAAAGATATTCTTCTAAATAATGAAGATTTATTAAACAAAGATAAAAGTTTTTGA", "aa": "MSTVKNNFDNVNELVKDILLNNEDLLNKDKSF", "aa_hexdigest": "16e0d69176099dc4f8a134ae5fe80175", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3725.1410000000005, "isoelectric_point": 4.528214836120605 }, "id": "EFPKPENHBP_144", "locus": "GCA000186005_00103" }, { "type": "cds", "sequence": "contig_1", "start": 53739, "stop": 54110, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATTTGTTGCCAGTAGAAAAAAAATCATTTGCTAAATATTTATTATATGCTTTCATAGAAATAGATAATATAGTTAAAAAAGGTAGTTGGTCAAGCAAAGTTAATCTTTTAGTAGAAACTACAAAATATATTCATTCAATAATAGATGCAAAAGCAGCAGCATTAGAAATAGCTTTAAATGTTGTCGGAGGTCCAGTTTCTGCTTTTGGTGGTTTATTGTCTAACTTTTATGTTAAATTTTACAAAGCATTATTTGGTGAATTTAGCCAATTTAACTATAAATACGTTGTCAATGCAGGAGATAAAGGTAATGTCGATTATATATGGAATGGTGGATTGCGTTATGTTGGTTTATTTCATTGA", "aa": "MKKDLLPVEKKSFAKYLLYAFIEIDNIVKKGSWSSKVNLLVETTKYIHSIIDAKAAALEIALNVVGGPVSAFGGLLSNFYVKFYKALFGEFSQFNYKYVVNAGDKGNVDYIWNGGLRYVGLFH", "aa_hexdigest": "c9ad9b5a9f148db23e3d8e95c12b079b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13798.869099999989, "isoelectric_point": 9.323756217956543 }, "id": "EFPKPENHBP_145", "locus": "GCA000186005_00104" }, { "type": "cds", "sequence": "contig_1", "start": 54165, "stop": 54341, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGGTTATACTGAAAGTTTTTATTACTTCAATGGTAAAAAATATAACTTTGTTGATATAGATTTATTAAAAAGAGATGTTGTAAATTACTATTTAAAAGGAAACAACAAAATTATAAACAACTTATTAGAAAAAGATGAAGCTGAAAAGAACATAAAAATTATTTACTCATGA", "aa": "MDGYTESFYYFNGKKYNFVDIDLLKRDVVNYYLKGNNKIINNLLEKDEAEKNIKIIYS", "aa_hexdigest": "5f30dddf43e072cd30bfbcd985bdd02e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6978.839899999999, "isoelectric_point": 6.139145469665528 }, "id": "EFPKPENHBP_146", "locus": "GCA000186005_00105" }, { "type": "cds", "sequence": "contig_1", "start": 54582, "stop": 55088, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTATTTATTATTCAAAAAAACCAATAGAAACAGAAGATGGTTACACCGACAAGCTATTTCCTATTGATCAATTTGATCCAACAAAAAAAGCTTTTGAAGACAAAAAAATACTAGCAAATATTTATAATGAAGATCCATTAAAAAATTGCTTTTTATATTCAGAAATAGGAAACTTTAATATTCAAAGCGATTTGTTAAAAAATAATAGAAACAAATTTATTCCATTTTTAAATTCATTGAATATAAGAACAAAAGAAGTTTCAAATATATCATTTTTTAATAATTCAAATTATCAAGATATGGAATTGTTGCAAACAAAAACACTTTATTATTTTGATTATAAAAACAATAAATACTTCTTTACAAATTTAGACAAAGCGAGAAATTATTTTATCAATGTTATTATTAACCCAAAAATGGAATTATATAATTTAACCAAAAGAACCAAAATTATATTTATGAATAAAGAGTTCTGTACATTGAATGAAGCAGTATCTTGA", "aa": "MNPIYYSKKPIETEDGYTDKLFPIDQFDPTKKAFEDKKILANIYNEDPLKNCFLYSEIGNFNIQSDLLKNNRNKFIPFLNSLNIRTKEVSNISFFNNSNYQDMELLQTKTLYYFDYKNNKYFFTNLDKARNYFINVIINPKMELYNLTKRTKIIFMNKEFCTLNEAVS", "aa_hexdigest": "7e129a86dc9ae178808e7d802a2d0e76", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.779200000008, "isoelectric_point": 8.669337654113772 }, "id": "EFPKPENHBP_147", "locus": "GCA000186005_00106" }, { "type": "cds", "sequence": "contig_1", "start": 55117, "stop": 55905, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAAATTATTTTTATCTTTAATTCCAACAGTTGTTTTGATTCCATCATGCAATAATTCAGCCTCTATGAATTATTATATGCAAAACAATAATTTAAAAAACGCAAGCAAATATGAAATTAATTTAGAAGGACAAAAATACCAATATACTGATATTGAATCATTTATGTCTAATTTAATAAATAGTAATAAAATAAAAGTTGAAAACAAATTAGTAATAGGCAAAAAGAAAAACAATAGTTCAAATAAAATTCTAGATTCTACTATTTTTTATAATTTCGACCCAAATAAACTTAAAAAAGTTTATCAAAATAAATTCCAAAAAACGGTTTATTCAGTTGATGAAGTAATAGAATCGTTTAGAGAAGAAAGCTCATATATTAAAAAATATCTTTTTGCCAATAAAGAATTTGATTCAGAAGAAAAAGTAAAACAGTATATAAAAACTCAACTTAAAAACGGTTTTAATAAATATGTTTATTATTCTAATAATGGGAAAAAATATAATGCTATGAATTGGCAAGATTCATTAGAATTGCTAAAAGATAAATATAATAAAGATAACAATGTGTTTTATCCTATTTTTGATAACAACAATAATGTAATTTATAATAATTTACTTAATTATGAAACAATGAATTCTATTAGTAAAAAAATTCTTGAAAAGTTAATGAAGTTTAAAGTAGAAATAAAATTTGGTTTAGATCTCAATGATGAAAAGTCTTATGACTACAATGAAAAGGATTTAGGTTTTAAAGTAAATGTAAAAAATAATAAATGA", "aa": "MKIKKLFLSLIPTVVLIPSCNNSASMNYYMQNNNLKNASKYEINLEGQKYQYTDIESFMSNLINSNKIKVENKLVIGKKKNNSSNKILDSTIFYNFDPNKLKKVYQNKFQKTVYSVDEVIESFREESSYIKKYLFANKEFDSEEKVKQYIKTQLKNGFNKYVYYSNNGKKYNAMNWQDSLELLKDKYNKDNNVFYPIFDNNNNVIYNNLLNYETMNSISKKILEKLMKFKVEIKFGLDLNDEKSYDYNEKDLGFKVNVKNNK", "aa_hexdigest": "466aad8a694c451aa6689ecf538d868b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31110.143100000023, "isoelectric_point": 9.340324592590331 }, "id": "EFPKPENHBP_148", "locus": "GCA000186005_00107" }, { "type": "cds", "sequence": "contig_1", "start": 56797, "stop": 57138, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTCAAAGAAAAACATGTATAGAATTTTTAAACAAAATAATTTCTTCTAAATCATTTCCAAGTGCTTCTGAACCCGAAGACAAACAAAAACTTTTAAAAGAAATTATTGAAGAACATAAGAATAAAAACAAATTAAATAATAGCTTTTTTGAAAATAATTTGCCCTTACTTATTAATGATAAAAACATTGAATTATTTTCTTTCTATTCAAATAATAATGTTGACATCTTATCAAAAAGATATTCAATACCTTCTAACATACTACTTGAGTTTCCAACTTTTTATTCTAAAGATGATTATGAAAATAACATAGCTCTTTATTTTGACGAAAATTGA", "aa": "MIFQRKTCIEFLNKIISSKSFPSASEPEDKQKLLKEIIEEHKNKNKLNNSFFENNLPLLINDKNIELFSFYSNNNVDILSKRYSIPSNILLEFPTFYSKDDYENNIALYFDEN", "aa_hexdigest": "847c1d7cacac38fcc8b6662a8cebaf44", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13409.995499999979, "isoelectric_point": 5.182545280456542 }, "id": "EFPKPENHBP_149", "locus": "GCA000186005_00108" }, { "type": "cds", "sequence": "contig_1", "start": 57223, "stop": 57426, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806470", "UniRef:UniRef90_UPI0021806470" ], "nt": "TTGAGATATTTACTTAGTAATCAATTAGGGTATAAAGAAGATTTAGTTGCATATGATAATAAAGGTGTAGTAGAAATAATTAACAATGACATTTTCAATGTGTTTGTATTGAAATTTGAAGGCAATGAGTTTTCATTCCTGAGTTATAATGATGCAATTTCATTTTTAAAAGATTACATAATAACAAGATCAAAATTAATATAA", "aa": "MRYLLSNQLGYKEDLVAYDNKGVVEIINNDIFNVFVLKFEGNEFSFLSYNDAISFLKDYIITRSKLI", "aa_hexdigest": "e1482f762bc20ed36c7d2630a9d9637d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021806470", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.985, "score": 131.0, "evalue": 7.65e-39, "valid": true, "uniref50_id": "UniRef50_UPI0021806470" }, "pscc": { "uniref50_id": "UniRef50_UPI0021806470", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806470" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7862.912499999995, "isoelectric_point": 4.810363578796387 }, "id": "EFPKPENHBP_150", "locus": "GCA000186005_00109" }, { "type": "cds", "sequence": "contig_1", "start": 57774, "stop": 58103, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "ATGAGTGATATTAATGCTAAATTAGTTTATGCAATTATCACACACATTAAAAACGAAGTTAGAGCTAGAGGTTTAAAAGATACTAAAGATATAGGTGAATTAGTTGCTGATCAAATGTTTGTTGTTTATACAAACAAAAGTATTGTTGATACAACTTTAAATTATCAAGACGGGAGATTAAATATCTTTATTTTTGTTGGAGTTAATGGTAGTGGTAAAACAACTTCAATTGCTAAAATAGCCTATAAATATATAAAAGAAGGAAAAAAAGTTTTAATTGCTGCAGGCGATACTTTTAGAGCTGGTGCAGTAGATCAATTAGGTGTGTGA", "aa": "MSDINAKLVYAIITHIKNEVRARGLKDTKDIGELVADQMFVVYTNKSIVDTTLNYQDGRLNIFIFVGVNGSGKTTSIAKIAYKYIKEGKKVLIAAGDTFRAGAVDQLGV", "aa_hexdigest": "54fa3717e7fe33575a41645ca6c556b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.44036697247706424, "hmm_cov": 1.0, "evalue": 9.21559649830197e-20, "score": 67.41764831542969, "start": 61, "stop": 108 } ], "seq_stats": { "molecular_weight": 11912.65809999999, "isoelectric_point": 9.362372779846194 }, "id": "EFPKPENHBP_151", "locus": "GCA000186005_00110" }, { "type": "cds", "sequence": "contig_1", "start": 58257, "stop": 58580, "strand": "+", "frame": 3, "gene": "ftsY", "product": "Cell division protein ftsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "nt": "ATGAAAGAACTTGAAAAAATGTATGGCATTATTGCTAGATTTCAAGATAAAGCGCCACATGAATGCTTATTAGTTTTAGATGCAACAACTGGTCAAAATGGAGTTAGCCAAGCTAGAAGTTTTAAAGAAGTTGCGGCTCCAACTGGAATTATATTAACTAAAATGGATGGAACTTCTAAAGGTGGAATTGTACTTTCAATTAAAGACGAATTTGATTTGAATGTTAAATATATTGGACTTGGCGAAGGGCTAGATGATCTTCAAGAATTTGATTTAGATAACTTCATTTATGAAATGACAAAGGACTTAATTGTAAATGAATAA", "aa": "MKELEKMYGIIARFQDKAPHECLLVLDATTGQNGVSQARSFKEVAAPTGIILTKMDGTSKGGIVLSIKDEFDLNVKYIGLGEGLDDLQEFDLDNFIYEMTKDLIVNE", "aa_hexdigest": "9c8e085c190240b900b91241932ea558", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PPJ9", "query_cov": 0.9719626168224299, "subject_cov": 0.9203539823008849, "identity": 0.76, "score": 157.0, "evalue": 9.43e-48, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_E4QS11" }, "pscc": { "uniref50_id": "UniRef50_E4QS11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "product": "Cell division protein ftsY" }, "genes": [ "ftsY" ], "id": "EFPKPENHBP_152", "locus": "GCA000186005_00111" }, { "type": "cds", "sequence": "contig_1", "start": 58573, "stop": 58803, "strand": "+", "frame": 1, "gene": null, "product": "Sigma-70, region 4", "db_xrefs": [ "KEGG:K09787", "RefSeq:WP_013354325.1", "SO:0001217", "UniParc:UPI0001A8C3BB", "UniRef:UniRef100_C4XE46", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_C4XE46" ], "nt": "ATGAATAAAGATATTAAAGATATAGAAAATAGAGAGCACTTATTAGAACTATTTGATAAATTTAATTCATTTTTAACTCAAACACAAAAACAAGCTTTTCAATTATATTTCATTGAAGATCTTTCTTATTCTGAAGTTGCCAACATTACAGCAACCACCCGTTCTGGAGCTTATGATAGTGTGAATAAAGCAATTAAAAAATTACTAGATATTGAAGCAAAAATGCAATAA", "aa": "MNKDIKDIENREHLLELFDKFNSFLTQTQKQAFQLYFIEDLSYSEVANITATTRSGAYDSVNKAIKKLLDIEAKMQ", "aa_hexdigest": "ca972cf6120e9f43c803240e82563c66", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C3BB", "ncbi_nrp_id": "WP_013354325.1", "uniref100_id": "UniRef100_C4XE46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3BB", "RefSeq:WP_013354325.1", "UniRef:UniRef100_C4XE46" ] }, "ips": { "uniref100_id": "UniRef100_C4XE46", "uniref90_id": "UniRef90_C4XE46" }, "psc": { "uniref90_id": "UniRef90_C4XE46", "product": "Sigma-70, region 4", "uniref50_id": "UniRef50_C4XE46", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [], "id": "EFPKPENHBP_153", "locus": "GCA000186005_00112" }, { "type": "cds", "sequence": "contig_1", "start": 59440, "stop": 59766, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAACTTAAACCTTTCAGCGAAAATGAATTAAAAGTGTTATTTGAAAAATTTAATGACTATATTATTGTTGGAGGAATGCCAGAGATTGTTAAAAATTTCATAAAGCAAAAAAATTTTTCAAATATTTTAAATCAACAAAAAGAATTACTTGAAGTTTATAAGCAAGACATTGGTAAATATGCAGAAAGTTTGGATAGATACAAAATTTTAAAAGTTTATGAAAGTATTTCTCCATTTTTAGGTAAAGAAAACAAAAGATTTATGTTTTCAAAAGTTGAACATAATGCAAGGTTTAGAGATTATTTTTCAACAATGGAATGA", "aa": "MKELKPFSENELKVLFEKFNDYIIVGGMPEIVKNFIKQKNFSNILNQQKELLEVYKQDIGKYAESLDRYKILKVYESISPFLGKENKRFMFSKVEHNARFRDYFSTME", "aa_hexdigest": "dacaee29711aa2e76541ce43d720af53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13003.91509999999, "isoelectric_point": 9.023849296569825 }, "id": "EFPKPENHBP_154", "locus": "GCA000186005_00113" }, { "type": "cds", "sequence": "contig_1", "start": 60328, "stop": 60867, "strand": "-", "frame": 1, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8LI57", "UniRef:UniRef50_C4XE44", "UniRef:UniRef90_C4XE44" ], "nt": "TTGCATACCTTAAATAAAAAGAATGAATTAATGAAAATAAGTTATTTAAAAAATGAACAAAATGAAAAATCATCTAAAGCTTGGTATGTTTTAACAACAAACAATTCATCTTATGGTCTTTTTGGCTTAAAAAACTATAAAACAGATGTTTTGGAAAAAAATTGTTTTAAAAAAATAAATTTAAAAAATAGATCTTTTATTAATTTTAATAATGACTTTGTACACTCTGCTTATACTTCAGACGATGAATTAAGATTATTAGAAATATCAGTTAATACTAAAAAAACTGAAGTAAAAAATTTTAAAGATTTAAATAAAATTAACAATGAAAATTCATCTACATCAATGAATAGATATTATAAGGAAGATTGTATTGTTTTTTACAATAAAAAAACTAGAATTACATTATTTAATGATAATAAAGAATATATCGTAAAAAGAAAATGTTTAGTTTTTGATTGCATCAGTGAAACGCTTTATAGAGCAAATAAAGGCGATGAACTAAACTTTCATAAAATGTTAATAATTGAAAAAAGATAA", "aa": "MHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKINLKNRSFINFNNDFVHSAYTSDDELRLLEISVNTKKTEVKNFKDLNKINNENSSTSMNRYYKEDCIVFYNKKTRITLFNDNKEYIVKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR", "aa_hexdigest": "b80e09c667bb6c244e28f358f999ec67", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8LI57", "query_cov": 0.9497206703910615, "subject_cov": 1.0059171597633136, "identity": 0.7, "score": 234.0, "evalue": 1.72e-76, "valid": false, "uniref50_id": "UniRef50_A0A3P8LI57" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI57" ] }, "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 60328, "stop": 61167, "observations": { "mutation": [ 61062 ], "stop": [ 61062 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE44", "query_cov": 1.0, "subject_cov": 0.5343283582089552, "identity": 0.9940000000000001, "score": 352.0, "evalue": 1.31e-120, "gene_start": 1, "gene_end": 179, "reference_start": 157, "reference_end": 335, "reference_sequence": "MKLIANKGDIMPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLNWRKVPELHFYIDEVSKKCLSIDRILYDIKDEKNFEANNKISYNEHLTNFGNSKIFELDKYEPQIVLHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKINLKNRSFINFNNDFVHSAYTSDDELRLLEISVNTKKTEVKNFKDLNKINNENSSTSMNRYYKEDCIVFYNKKTRITLFNDNKEYIVKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR" }, "paralog": false, "identity": 0.989247311827957, "score": 555.0, "evalue": 5.24e-203, "description": "Internal stop codon at 61062. Nonsense mutation around 61062.", "psc": { "uniref90_id": "UniRef90_C4XE44", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_C4XE44", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE44", "UniRef:UniRef50_C4XE44", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE44" ], "product": "Ribosome-binding factor A" } }, "id": "EFPKPENHBP_155", "locus": "GCA000186005_00114" }, { "type": "cds", "sequence": "contig_1", "start": 61060, "stop": 61305, "strand": "-", "frame": 1, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8L789", "UniRef:UniRef90_A0A3P8L789" ], "nt": "ATGCCTAAAAGTATAAACACATTAAGAAGAGAATCACAAATTAGAACATTATTAGCAGAAATAATAACAAATGATTTAACAAATACAAATATTATTAATCCTACATTGGTTGATTGCGAATTATCAGGTGATTTATCACATGTAAAAATCTATATGTCATTTAGCGAAAATGAAAAGAGAGGATTAGAAGCTTTACAAAATGCTTCTGGATACATTAGAACAATTTTGGCTAAAAGTTTAAATTGA", "aa": "MPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLN", "aa_hexdigest": "5888db5fd4d08b4f705c51a66dd6f053", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rbfA" ], "truncated": "5-prime", "pseudogene": { "start": 60907, "stop": 61305, "observations": { "mutation": [ 61062 ], "stop": [ 61062 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8L789", "query_cov": 1.0, "subject_cov": 0.5664335664335665, "identity": 0.889, "score": 142.0, "evalue": 5.04e-42, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MAKNINVLRRESQIRTLLAQIITNDLTNTNIINPTLVDCELSADLSHVKVYMSFSENEKRGLEALQNASGYIRSSLSKSLNWRKVPELHFYIDEVSKKGLSIDKILYNIRDKENFETNNKVSFEQKETNYGNDVNPKTCTKKL" }, "paralog": false, "identity": 0.8333333333333334, "score": 224.0, "evalue": 1.22e-76, "description": "Internal stop codon at 61062. Nonsense mutation around 61062.", "psc": { "uniref90_id": "UniRef90_A0A3P8L789", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A3P8L789", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8L789", "UniRef:UniRef50_A0A3P8L789", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L789" ], "product": "Ribosome-binding factor A" } }, "id": "EFPKPENHBP_156", "locus": "GCA000186005_00115" }, { "type": "cds", "sequence": "contig_1", "start": 61346, "stop": 61738, "strand": "-", "frame": 3, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43", "UniRef:UniRef90_C4XE43" ], "nt": "ATGAATTTAGTTTCTAATTTATTAAAAATTGGTAAACCTGTTATAGCTCATAGTGACTTTCATATTTTCTTTCCATCCTTAGCTCATGTTACTGAAAATGATGTTGTAATTGTTTACTCAAATAATTTACAAACAATGGAAGCTCACTTTGTTATCGAGCAAGCTAAAAAACAAAAAGCGCCAATTATTGTTTTAACTTCTAGCCAAGAAGAAGATAAATTAATTGCAGTTAAATTAAGATATCACAAAATTCAAAGTTCAACTATGTTAGTTCCTTCAAGTTCAAAAATAGCTCAAATGTTAATTACCGATTTATTATTTGAAGCTGTTTTAGAGCATAATAAGGAAAATACAAGTAAACTTAAAAAAGCAAGAATACTTATTAAAAATTGA", "aa": "MNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKN", "aa_hexdigest": "dcf30baf8efb0cff998da307d29276ff", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpiR" ], "truncated": "both", "pseudogene": { "start": 61316, "stop": 62038, "observations": { "mutation": [ 61348, 61912 ], "stop": [ 61348, 61912 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE43", "query_cov": 1.0, "subject_cov": 0.4377104377104377, "identity": 1.0, "score": 246.0, "evalue": 4.33e-80, "gene_start": 1, "gene_end": 130, "reference_start": 158, "reference_end": 287, "reference_sequence": "MKKFNIITIMKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTIWNDNEVDNDYKFIAASHRYAIDNVYDEDLLKDINKAAEIINKSKNIYIHGCGSSQRISMNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKNWSLIDKAHSE" }, "paralog": false, "identity": 0.9916666666666667, "score": 462.0, "evalue": 1.34e-167, "description": "Internal stop codon at 61348, 61912. Nonsense mutation around 61348, 61912.", "psc": { "uniref90_id": "UniRef90_C4XE43", "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE43", "UniRef:UniRef50_C4XE43" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43" ], "product": "RpiR family transcriptional regulator" } }, "id": "EFPKPENHBP_157", "locus": "GCA000186005_00116" }, { "type": "cds", "sequence": "contig_1", "start": 61910, "stop": 62182, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01418.23" ], "nt": "ATGAAAAATAATGACAAAAGAATAATAAGCCGTCCATTAATGGATGATACAGTTGAATTAACTAAAAGTGAAAAAAGAATTCGAGATTTTATTAATAATTTTGAAGGTAATTATTTTAACTTTTCACAAAATAAGTTAGCTAAAAATACTGAATCAAGTGATGCGGGAGTTTCTAGATTTATTCGTAAATATGGTTTTAAAGACTATCGCACTTTTATTGCTTCAATAAATAACAAATTAACAGAATTTGAAAAAACTTATCCTACTATTTGA", "aa": "MKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTI", "aa_hexdigest": "82241fe3d94061dd2cd7df428f06b7f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01418.23", "name": "HTH_6", "length": 60, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.608574536832507e-11, "score": 39.7202033996582, "start": 18, "stop": 77 } ], "seq_stats": { "molecular_weight": 10671.916599999988, "isoelectric_point": 9.598262596130368 }, "id": "EFPKPENHBP_158", "locus": "GCA000186005_00117" }, { "type": "cds", "sequence": "contig_1", "start": 62422, "stop": 62589, "strand": "+", "frame": 1, "gene": null, "product": "30S ribosomal protein S16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "nt": "ATGGTTAAAATTAGATTAAAGAGATTAGGAAGCAAATTTAATGCTTGCTACAAAATTGTGGCTGCTGACTCACACGCTCCTAGAGATGGGAAATATATTGAAGCATTAGGCCAATATAATCCACACTCAAAGGAATTTGTTTTAAATGAAGAAGCAACACAAAGATGA", "aa": "MVKIRLKRLGSKFNACYKIVAADSHAPRDGKYIEALGQYNPHSKEFVLNEEATQR", "aa_hexdigest": "78461022c437652e957a87f8934ac54f", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FB42", "query_cov": 0.8727272727272727, "subject_cov": 0.8727272727272727, "identity": 0.688, "score": 75.5, "evalue": 4.6e-17, "valid": false, "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A0A432FB42", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FB42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "product": "30S ribosomal protein S16" }, "genes": [], "id": "EFPKPENHBP_159", "locus": "GCA000186005_00118" }, { "type": "cds", "sequence": "contig_1", "start": 62662, "stop": 62868, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACGCACACAAAGAAGCTACAAAAGCACAAGAAGTTAAAAAACTTGCAGCTAAACCAGCTACAAAACCTGCTTCAAAATCAACTGCTCCTAAAAAAGTAGTTGCTGACAAAAAAGTTGCAACAAAAGCAGCAACAACTAAAACTACAAAAGCTGCTGCTAAACCTTCAGCTAAAACTGTTCGTAAAACTACAAAAAAATAG", "aa": "MNNAHKEATKAQEVKKLAAKPATKPASKSTAPKKVVADKKVATKAATTKTTKAAAKPSAKTVRKTTKK", "aa_hexdigest": "362355c0a988b88a03a798e7950e342f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.3544000000065, "isoelectric_point": 10.71852970123291 }, "id": "EFPKPENHBP_160", "locus": "GCA000186005_00119" }, { "type": "cds", "sequence": "contig_1", "start": 62882, "stop": 63583, "strand": "+", "frame": 2, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "RefSeq:WP_013354331.1", "SO:0001217", "UniParc:UPI0001E32F1D", "UniRef:UniRef100_C4XE41", "UniRef:UniRef50_Q98Q98", "UniRef:UniRef90_C4XE41" ], "nt": "ATGAAAATAAATTTTTTAACTCTTTTTTCAAATTACTACAAACCATTTGTAGAAGAATCAATTATTTCAAAAGCTATTGAAAAGAAATTGATTGAAATTAATGTAGTTGATTTTAGACTTTTCAGCAAAGATAAACATCACAAAGTTGATGATGAAATTTTTGGTGGAGGTCATGGAATGCTCCTGCAAATAGAACCTATTGATTTAGCACTTGATTCTCTTAAAGAAAGAGGAGGTTACAAAATTCTTGTAACTCCTCAAGGAAAGAAATTTGATCAACAAATTGCTAATGAATTAGCTAAGAAAAAAGAAATAACTTTTATTTCTGGTCGCTATGAAGGCTTTGATGAAAGAATAGTAGACCTTGTTGATGAAGAAATTTCAATTGGTGATTATGTTTTAACCGGAGGCGAATTACCTTCAATGGTAATGGCAGACAGTATCATAAGATTAGTTCCTGGCGTAATAAGAGAAGAAAGTTATGCTAATGATTCATTTCAAAATGAAGGTTTGTTAGATTATCCTCAATACACTAGACCAAGAGTGTACAAGAGCAAAGCTAAAAAAGAAGCTTTAATAGTGCCTGAAGTTTTAGTTAATGGAAATCATGCAGAAATTGAAAAATGGAAAAAAGAAGCTCAATTAAAAAAGACAAAGAAAAATCGTCCAGATATAATCGAAAGGATTAAAAATGAGAAATAA", "aa": "MKINFLTLFSNYYKPFVEESIISKAIEKKLIEINVVDFRLFSKDKHHKVDDEIFGGGHGMLLQIEPIDLALDSLKERGGYKILVTPQGKKFDQQIANELAKKKEITFISGRYEGFDERIVDLVDEEISIGDYVLTGGELPSMVMADSIIRLVPGVIREESYANDSFQNEGLLDYPQYTRPRVYKSKAKKEALIVPEVLVNGNHAEIEKWKKEAQLKKTKKNRPDIIERIKNEK", "aa_hexdigest": "cf67f528c10da360f56575dc0b732524", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F1D", "ncbi_nrp_id": "WP_013354331.1", "uniref100_id": "UniRef100_C4XE41", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F1D", "RefSeq:WP_013354331.1", "UniRef:UniRef100_C4XE41" ] }, "ips": { "uniref100_id": "UniRef100_C4XE41", "uniref90_id": "UniRef90_C4XE41" }, "psc": { "uniref90_id": "UniRef90_C4XE41", "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_Q98Q98", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q98" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "EFPKPENHBP_161", "locus": "GCA000186005_00120" }, { "type": "cds", "sequence": "contig_1", "start": 63573, "stop": 63929, "strand": "+", "frame": 3, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_013354332.1", "SO:0001217", "UniParc:UPI0001A8C3B5", "UniRef:UniRef100_C4XE40", "UniRef:UniRef50_O31742", "UniRef:UniRef90_A5IZ68" ], "nt": "ATGAGAAATAAATTAATCGAATTAGTAGAATCAAACCAATTACGTACAGACTTCCCAGAATTCAGAGTTGGAGATAATGTTAAAGTTCACGTTCGTATTCGTGAAGGTGAAAAAGAACGTATCCAAATTTTTGAAGGTTTAGTTATTAGCAAAAAAGAATCAGGTACACGTGAAACATTTACAGTTAGAAAAATTTCTTTTGGTATAGGTGTTAATAGAACATTTCCATTACACTCACCTATGATCGCTTCAATTGAAGTTGTTCGTTCAAATAAAGTACGTCGTGCAAAACTTTACTACATGAAAAACAGAAGTGGTAAAAGTGCTCGTCTTAAGGAAATTAAACGTACAAAATAA", "aa": "MRNKLIELVESNQLRTDFPEFRVGDNVKVHVRIREGEKERIQIFEGLVISKKESGTRETFTVRKISFGIGVNRTFPLHSPMIASIEVVRSNKVRRAKLYYMKNRSGKSARLKEIKRTK", "aa_hexdigest": "1dd843e14e47f79b7c4df38568a503bd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C3B5", "ncbi_nrp_id": "WP_013354332.1", "uniref100_id": "UniRef100_C4XE40", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3B5", "RefSeq:WP_013354332.1", "UniRef:UniRef100_C4XE40" ] }, "ips": { "uniref100_id": "UniRef100_C4XE40", "uniref90_id": "UniRef90_A5IZ68" }, "psc": { "uniref90_id": "UniRef90_A5IZ68", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_O31742", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O31742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O31742" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "EFPKPENHBP_162", "locus": "GCA000186005_00121" }, { "type": "cds", "sequence": "contig_1", "start": 64092, "stop": 64226, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGGAGTTTCATATATTGAACTTTTAGTTGATGGTAAAACTGTAGATAAATTAAATGTTTTTAAGAAAAGAAATGCTTCATTAAGTCATAATTTAGATGGCGACCTAATTCATGTTAGAATTGATGGCTGA", "aa": "MVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDG", "aa_hexdigest": "0cd091e3115818f9704f451dec542a25", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4880.5796, "isoelectric_point": 6.700998878479004 }, "id": "EFPKPENHBP_163", "locus": "GCA000186005_00122" }, { "type": "cds", "sequence": "contig_1", "start": 64293, "stop": 64487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39", "UniRef:UniRef90_C4XE39" ], "nt": "ATGTCTGAAAAAGGAAAAAAATTTAAAAAGAAACGTACAGGAATGATAATTGCTAGTTTAATATTTCTTTTTATAAGTATTGGATTAGCTGGAGCATTATTTCCAATAATTCATATAGTTGTTACAACTAAAAATTCAATTGGTTCAATAATAGCCATTATCATATCAGCTGTTTTTTTCATAATAACAATTTGA", "aa": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITI", "aa_hexdigest": "9d77ab5e6f8731718071735c0a82944e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64047, "stop": 64487, "observations": { "mutation": [ 64094, 64295 ], "stop": [ 64094, 64295 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE39", "query_cov": 1.0, "subject_cov": 0.4383561643835616, "identity": 1.0, "score": 108.0, "evalue": 6.46e-29, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITIWFINFSIGTNYKVVNYKDKTIEVLVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDGWVFYQVNIKINNELL" }, "paralog": false, "identity": 0.9863013698630136, "score": 273.0, "evalue": 1.9e-96, "description": "Internal stop codon at 64094, 64295. Nonsense mutation around 64094, 64295.", "psc": { "uniref90_id": "UniRef90_C4XE39", "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE39", "UniRef:UniRef50_C4XE39" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6935.540999999996, "isoelectric_point": 10.751730918884277 }, "id": "EFPKPENHBP_164", "locus": "GCA000186005_00123" }, { "type": "cds", "sequence": "contig_1", "start": 64522, "stop": 64968, "strand": "-", "frame": 1, "gene": null, "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_013354334.1", "SO:0001217", "UniParc:UPI0001E32F40", "UniRef:UniRef100_C4XE38", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_C4XE38" ], "nt": "ATGCATAAGATTAAAATAATTGCAGTTGGTTCACTTAGTCCAGAATTTAAAGTTTTATTTAATCAATATGCTAAAAACATAAGTCATTTTGCTGAATTAAATGTTGTTGAAGTAAAAGAATTGAGTGAGGAAAAAAATATCGAAGTCAAGAAAGACAAGGAAACAAAATTAATTAATTCTTATGTTGATAATAATAGTTTTGTTATTTATGCTTCATTAAAAGGAAAAAGTTTAGATTCAGTTGAATTAAGCGAAATAGTTAATAACAATGTTAATTTAACCTTTATAATTGGCGGCAGTGATGGAGTTAATGAAGAATTAATTAAATGCAATATGAAAATTAATTCTTCAAAATTAACTTGGCCACATCAATTGTTTAGAGTTATGTTAGCTGAACAAATCTATCGTTCATTTATGATCATTAATAATAAAAAGTATCATAAATAA", "aa": "MHKIKIIAVGSLSPEFKVLFNQYAKNISHFAELNVVEVKELSEEKNIEVKKDKETKLINSYVDNNSFVIYASLKGKSLDSVELSEIVNNNVNLTFIIGGSDGVNEELIKCNMKINSSKLTWPHQLFRVMLAEQIYRSFMIINNKKYHK", "aa_hexdigest": "5a24f8abde1e52ce51a1ab84206930eb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F40", "ncbi_nrp_id": "WP_013354334.1", "uniref100_id": "UniRef100_C4XE38", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F40", "RefSeq:WP_013354334.1", "UniRef:UniRef100_C4XE38" ] }, "ips": { "uniref100_id": "UniRef100_C4XE38", "uniref90_id": "UniRef90_C4XE38" }, "psc": { "uniref90_id": "UniRef90_C4XE38", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [], "id": "EFPKPENHBP_165", "locus": "GCA000186005_00124" }, { "type": "cds", "sequence": "contig_1", "start": 64961, "stop": 66814, "strand": "-", "frame": 3, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "GO:0002098", "GO:0008033", "KEGG:K03495", "RefSeq:WP_013526627.1", "SO:0001217", "UniParc:UPI0001F59AAE", "UniRef:UniRef100_C4XE37", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_C4XE37" ], "nt": "ATGAATAAGAAAAGTAATAATGTACATAAATATGATGCTGTTGTTATAGGTGGAGGGCACGCTGGTTTAGAAGCTGCTTTTGCCTTAGCACACAAAAATCATAAAACTGTTTTGATATCTTTAAATAAGGAACGTTTAGGTATGATGCCATGTAATCCTTCAATTGGTGGACCAGCCAAAGGAATCATAACTAGAGAAATAGATGCCTTAGGCGGAATGCAAGGACTTTGGGCTGATTTAAGCATGATCCAATTGAAAATGCTTAATCAATCAAAAGGTCCTGCAGTTAGAGCACTTAGAGCTCAAATTGACAAAGAAAAATACATGAAGATAGCTTTAGAATACGTGTTAAAAGAACCTAACTTAGATCTTATTGAAGATATAGCTGAAGAAATATTAGTAAATAGTAAAAAAGAATTTAAAGGAATTAAACTAGCTAATGGCGATATAGTTGAAGCTGAGGTCTGTGTTGTAACTACTGGAACATATATGGACAGTAGAATTCTTAGAGGAGATGTTATTAAAATCAGTGGACCTGATAATGAAAAAACTACACCTAAATTAAGTGCTTCATTAAAAGAACATGGTTTTATAATTCAAAGACTTAAAACTGGAACACCTCCAAGAATCTTTTCAGATTCAATTGACTACTCGCAAGTAGAACAAGAAGTATTAAGTGATACTAACTTATCATTTTCATCTAGATCAAATGTAAAATTACCTCGTCAAATTGCTTGTTACTTAACTTATACAACTCCTGAAACTCACAAAATAATAGAAGAAAATTTAACTAAAAGTGCTATGTATGGAGGAATAATCAAAGGTATTGGCCCTCGTTATTGTCCTTCAGTTGAAGACAAAATTGTTAAGTTTCCTTCAAAAGAAAGACATCAAATTTTCTTCGAACCTGAAACTGCTGATGGTTCAATTACTTATGTTAATGGGCTCTCTACTTCAATGCCAGTTGAAGTCCAAGAACTAATGATTAGAAGTATTCCTGGTCTTCAAAAATGTAAGATTCAAAAGTATGCTTATGCTATTGAATATGATGCAATTGATCCTTTGCAATTAAAACCTACTTTAGAAACTAAAATCATTAAAAATTTTTATACTGCAGGTCAAATTAATGGAACTAGTGGTTACGAAGAGGCTGCTGCTCAAGGTTTAATTGCAGGTATTAATGCAGCCTTAAACTTAGAAAAAAAAGATGGTATAGTTATTTTAAGAAACCATGCTTATATAGGAGTTTTAATTGATGATTTGGTTACTAAAGGAACTAAAGAACCTTATAGAATGCTAACTTCAAGAGCTGAATATAGATTACTTTTAAGAAATGATAATGCTGATATTCGTTTAGCCGAATATGGCTATAAAATAGGCTTAATTGACAAACATCAATATCAAAAGGTTATTAAAAAATATGAATTAATTGACCAAGAAATTCAAAGACTTCATACTACTTATCTTTCTTCAAAAGATCCTGTGGCTATAAAATATGGAATCACAACAGGTATCTCATTATTACAAACTTTAGCTCGTCCTGCAGTTGATCCTTATGATATCATTCCTGATTTTCCATACCTAGAAGAATTAACAACAATGGTTAGACTTCATGGTTATATTGAAAAACAAAAAAGTGATGCTAATAAAGCTGTAAGACTAGAAAATTACAAAATACCAGAAGACTTGGACTACAACAAAATTAATAATATTGCTACAGAAGCTAAACAAAAATTAATCAAAGTTAAACCAACAACAATCGGCCAAGCAAGTAGAATCAGTGGCATTAATCCAGCTGATATTCAAATGCTTATGTTTTATTTAGAAACTACAAGAAAAAAGAATAATGCATAA", "aa": "MNKKSNNVHKYDAVVIGGGHAGLEAAFALAHKNHKTVLISLNKERLGMMPCNPSIGGPAKGIITREIDALGGMQGLWADLSMIQLKMLNQSKGPAVRALRAQIDKEKYMKIALEYVLKEPNLDLIEDIAEEILVNSKKEFKGIKLANGDIVEAEVCVVTTGTYMDSRILRGDVIKISGPDNEKTTPKLSASLKEHGFIIQRLKTGTPPRIFSDSIDYSQVEQEVLSDTNLSFSSRSNVKLPRQIACYLTYTTPETHKIIEENLTKSAMYGGIIKGIGPRYCPSVEDKIVKFPSKERHQIFFEPETADGSITYVNGLSTSMPVEVQELMIRSIPGLQKCKIQKYAYAIEYDAIDPLQLKPTLETKIIKNFYTAGQINGTSGYEEAAAQGLIAGINAALNLEKKDGIVILRNHAYIGVLIDDLVTKGTKEPYRMLTSRAEYRLLLRNDNADIRLAEYGYKIGLIDKHQYQKVIKKYELIDQEIQRLHTTYLSSKDPVAIKYGITTGISLLQTLARPAVDPYDIIPDFPYLEELTTMVRLHGYIEKQKSDANKAVRLENYKIPEDLDYNKINNIATEAKQKLIKVKPTTIGQASRISGINPADIQMLMFYLETTRKKNNA", "aa_hexdigest": "23272e9ffd7deed5b575b5c56c0a4d03", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59AAE", "ncbi_nrp_id": "WP_013526627.1", "uniref100_id": "UniRef100_C4XE37", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59AAE", "RefSeq:WP_013526627.1", "UniRef:UniRef100_C4XE37" ] }, "ips": { "uniref100_id": "UniRef100_C4XE37", "uniref90_id": "UniRef90_C4XE37" }, "psc": { "uniref90_id": "UniRef90_C4XE37", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "EFPKPENHBP_166", "locus": "GCA000186005_00125" }, { "type": "tRNA", "sequence": "contig_1", "start": 66877, "stop": 66953, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.4, "nt": "GCGGATGTGGTGAAGTGGTCTAACACAGCGGGTTGTGGTCCCGTCATTCGAGGGTTCGAATCCCTTCATCCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 66883, 66885 ], "id": "EFPKPENHBP_4", "locus": "GCA000186005_00126" }, { "type": "cds", "sequence": "contig_1", "start": 67137, "stop": 67571, "strand": "+", "frame": 3, "gene": null, "product": "DHH family phosphoesterase", "db_xrefs": [ "EC:3.1.13.3", "EC:3.1.3.7", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_A0A449B7A5", "UniRef:UniRef90_C4XE36" ], "nt": "ATGAAAATAGGAAATTATAAAGTTGCATTACAAGCAATCGAAAAATATAAGAATATTATTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATAAAAAAGAATTATCCAAAGAAAAAAGTTTTTACAGTTGGAGATAATGTTCATACTTTTGATTTTATGAGTTACCACTATGACCCAATTGAAACGGTTGATTTTAAAAATTCATTAGGTATTGTAGTTGATGCTTCAAGTAGTGATCGGATTCAAGATGGACATTTATTAGTAGAAAAAAAATGTACAGCTGCTTTAAGAATTGATCACCATCCAAATGGTTCAGATATCGAATATGATTATTTATGGATCGATGAACACTATGTAGCTGCGGCTGAAATGGTGGCTATGTTAGCTAAAAGTGCAAAATGA", "aa": "MKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAK", "aa_hexdigest": "323886bd708bbedc1b7ebd8def08d762", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 67110, "stop": 67874, "observations": { "mutation": [ 67569, 67710 ], "stop": [ 67569, 67710 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE36", "query_cov": 1.0, "subject_cov": 0.4350453172205438, "identity": 1.0, "score": 297.0, "evalue": 2.09e-99, "gene_start": 1, "gene_end": 144, "reference_start": 10, "reference_end": 153, "reference_sequence": "MNIKLKEKNMKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAKWEITKKAAEHIYLGINTDSGRFLYPDTSARTYKLVAHLIEKGGLDSQWILRELNKRSSRDIKYTGEILSGFKQSGRVLYFEVTKEIMDKYGMNDLEAAIFVNELANIDDNSCWAFFIQLADGTVRCRLRSNGPLVNNVARLHNGGGHDNAAGCSINSWDEVPVVLKELNDCIKEWEAK" }, "paralog": false, "identity": 0.9881889763779528, "score": 503.0, "evalue": 8.27e-183, "description": "Internal stop codon at 67569, 67710. Nonsense mutation around 67569, 67710.", "psc": { "uniref90_id": "UniRef90_C4XE36", "product": "DHH family phosphoesterase", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_A0A449B7A5", "kegg_orthology_id": "K06881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE36", "EC:3.1.13.3", "EC:3.1.3.7", "UniRef:UniRef50_A0A449B7A5", "KEGG:K06881" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7A5" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase nrnA" } }, "id": "EFPKPENHBP_167", "locus": "GCA000186005_00127" }, { "type": "cds", "sequence": "contig_1", "start": 68105, "stop": 68488, "strand": "+", "frame": 2, "gene": "nrnA", "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "nt": "ATGGGACACATGAAAATTGGTAGCTTAAAAGAAGCAACAAAATTAATTAAAAAATATGACTCAATAGTTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATTAGAGCTAATTTTCCAGAAAAGAAAGTTTACACAGTTGGCGATAATGAAGGTATGTTTCCATTTTTAAATCTTAAATATGATAAATGTCCTTCAGATAATGTACTTAAAAAATCTTTAGGCATTATTCCGGATGCAAATGGTAAAGAAAGAATTCAATGTCGAGAAATTTTAGATAAAAACTTATTTGCTGAAACACTTAGAATTGATCATCATCCAAATGGTGATGACTTAGATAAATGTACCAGATGA", "aa": "MGHMKIGSLKEATKLIKKYDSIVIFHHIRPDGDCLGSQAGLAELIRANFPEKKVYTVGDNEGMFPFLNLKYDKCPSDNVLKKSLGIIPDANGKERIQCREILDKNLFAETLRIDHHPNGDDLDKCTR", "aa_hexdigest": "1ef3fc7e774a6e89631548d43958c6ca", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024C6B900", "query_cov": 0.984251968503937, "subject_cov": 0.8741258741258742, "identity": 0.6559999999999999, "score": 175.0, "evalue": 3.75e-54, "valid": false, "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "uniref50_id": "UniRef50_UPI0024C6B900" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C6B900", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "EFPKPENHBP_168", "locus": "GCA000186005_00128" }, { "type": "cds", "sequence": "contig_1", "start": 68744, "stop": 68968, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATAATTTAAAAACAGTTGGTAAAGTTGCTTATACAACTATTGATTTAAAAACAACAAAAGCTTTAGGCAAAAAACCAAATCAAGTTGTTAGAGTAAATACAATTGGGAATATTATTGGTTATCCATTTTGGGTTCAATTCAATGAAGAAGAAAATGGCAATGTTAGAATTGAATTTAGATCAAGTGGTCCTTGTGTTAGAAATGTTGCAGTTAAATGA", "aa": "MLNNLKTVGKVAYTTIDLKTTKALGKKPNQVVRVNTIGNIIGYPFWVQFNEEENGNVRIEFRSSGPCVRNVAVK", "aa_hexdigest": "2970673e9479e747f725cac31bca1e1e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8280.499499999998, "isoelectric_point": 9.917961311340331 }, "id": "EFPKPENHBP_169", "locus": "GCA000186005_00129" }, { "type": "cds", "sequence": "contig_1", "start": 69306, "stop": 69578, "strand": "+", "frame": 3, "gene": "rimP", "product": "Ribosome maturation factor RimP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34", "UniRef:UniRef90_C4XE34" ], "nt": "TTGTTAGACATTAGTGTCAATTATCATGACTTAAATAAGGTTGAAGAAATATCAAAACAACTTAATGAATTTATAGATTCTAATTATCCATCTTTGGATATTGATTCATTATCAATACATTCTCCAGGTGTCGAATTAACCTATAAAACAGATGAATTAGGAAAACACATTGGAGAAATGTTAACTATAAAATTAAATAAAAGTATTGAAAAACAAGACAAATTTAATGGTAAATTATTAGAAGTTTTAGAAGGCAAAATTCAAATAAGTTGA", "aa": "MLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQIS", "aa_hexdigest": "91f3260c4d36865ff043a97367d2ffa2", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "rimP" ], "truncated": "both", "pseudogene": { "start": 69027, "stop": 69659, "observations": { "mutation": [ 69150, 69171, 69231, 69576 ], "stop": [ 69150, 69171, 69231, 69576 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE34", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.9890000000000001, "score": 173.0, "evalue": 3.89e-53, "gene_start": 1, "gene_end": 90, "reference_start": 94, "reference_end": 183, "reference_sequence": "MQLLKTVMRKLNVEKEKATTTKKNNFKTCTSASFFVDLKILWYNKTIKWILQSCNLQLFVIFVGGFMNWKEILEKQFGSQILEAKIIEEDDLKLLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQISWNNKGQFRKVFIDKNNIVKVEKYIKFN" }, "paralog": false, "identity": 0.9809523809523809, "score": 397.0, "evalue": 4.7e-144, "description": "Internal stop codon at 69150, 69171, 69231, 69576. Nonsense mutation around 69150, 69171, 69231, 69576.", "psc": { "uniref90_id": "UniRef90_C4XE34", "product": "Ribosome maturation factor RimP", "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE34", "UniRef:UniRef50_C4XE34" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34" ], "product": "Ribosome maturation factor RimP" } }, "id": "EFPKPENHBP_170", "locus": "GCA000186005_00130" }, { "type": "cds", "sequence": "contig_1", "start": 69735, "stop": 70016, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "TTGATAGGTTATTTTGAAAAAGAAAAACTTCCTATTGAAGAAGTTATCAAAATTTTTTCAGAAGAAACTACACGAATTATTAACAAAATCTTAGATCCAAATGCTGAAATAGTTTTTGAATTAGACAAAGAAAAACAAGAAGTTCACATTTTTAACAACAAAACATGTGTAACTAAAGATGAAGAATTATTGCTTGATCTTGAAAATCAAATAAGTTTTGTTGGATATAAAGAAGCAAAAGTAAGAATGCCTAAAGTTAAAGAAGATGATGAAATTAAATGA", "aa": "MIGYFEKEKLPIEEVIKIFSEETTRIINKILDPNAEIVFELDKEKQEVHIFNNKTCVTKDEELLLDLENQISFVGYKEAKVRMPKVKEDDEIK", "aa_hexdigest": "b4eb22474a87a29a35e377925828c3e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 88, "aa_cov": 0.9354838709677419, "hmm_cov": 0.9204545454545454, "evalue": 2.5567925340891687e-07, "score": 27.253284454345703, "start": 6, "stop": 92 } ], "seq_stats": { "molecular_weight": 11001.563999999995, "isoelectric_point": 4.7830808639526365 }, "id": "EFPKPENHBP_171", "locus": "GCA000186005_00131" }, { "type": "cds", "sequence": "contig_1", "start": 70218, "stop": 70376, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAATGTTAATGCATATTTACCAGCAGGATTAATTAGTACAAAAAGAAAAGCAAACCCAGGTCAATATTTTGATGTTGTTATTGAAAAAGTTTCAGAAGAAACAAAATTGAGTCAAATTGAAGTTTCATTAGACTCACCTAAAATTGTTTGA", "aa": "MNSNVNAYLPAGLISTKRKANPGQYFDVVIEKVSEETKLSQIEVSLDSPKIV", "aa_hexdigest": "2af832be75027ba1af4df14ab9309e20", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5722.4785999999995, "isoelectric_point": 6.090263938903808 }, "id": "EFPKPENHBP_172", "locus": "GCA000186005_00132" }, { "type": "cds", "sequence": "contig_1", "start": 71019, "stop": 71285, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCATTACAAGAAGATGACTCAAACTTCGAAGAATTAGTTAAGAAAATTGAAAGCGAATATGCAGTTGAAAATGAAGTAGAAAACATTAAAAATGTTGAAGTAACAAAACCAGTTGCTCAAGATAACAAAATTTCCGCATCTGACTATAAAAAAGCTAAAGAAGTCGCAAAAGACTTTGTTGTTGATGATGATTTAAAGAATTTTGGAATTGACGATTTTGATTTAAGTGACATTGATTTTGATGAAGAAGAAAACAAATAA", "aa": "MDSLQEDDSNFEELVKKIESEYAVENEVENIKNVEVTKPVAQDNKISASDYKKAKEVAKDFVVDDDLKNFGIDDFDLSDIDFDEEENK", "aa_hexdigest": "1994556343f9dcef3bd3cc6ad8fea579", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10124.812699999997, "isoelectric_point": 4.052472496032714 }, "id": "EFPKPENHBP_173", "locus": "GCA000186005_00133" }, { "type": "cds", "sequence": "contig_1", "start": 71559, "stop": 71720, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAAAGAACAGAATAAGTAACGTTGATGAAGTCAAGAACCAACTTTCAGATATTAAAACTGAAGTTAAGAATGGTGTTTTCATTTTTACAAACAAAATGCCATTAGGAGAATTTGCACAAAAAATTGGTATGAATGCCAATGATTTGATCAAGTGA", "aa": "MPRKNRISNVDEVKNQLSDIKTEVKNGVFIFTNKMPLGEFAQKIGMNANDLIK", "aa_hexdigest": "3364c9d5629f3127b5f868b7a157eec0", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6035.9703, "isoelectric_point": 9.600390052795408 }, "id": "EFPKPENHBP_174", "locus": "GCA000186005_00134" }, { "type": "cds", "sequence": "contig_1", "start": 71727, "stop": 73364, "strand": "+", "frame": 3, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_A0A3P8LI10" ], "nt": "ATGAAAGGAAAGCTTTATCAAATTAACTACGTACTTGATGAAGAAGAAATTGCTGAAGTATGTATGGAAAGAGGACTTGACTTTAGAAAAGAACAAAACATTGATGCTGCAAATTTTTTGCATGCAGTTAAATTTGAAGATGATGAAAAATCATTAGTAAAAAGACCTCCAATTATTACAGTTATGGGTCATGTTGATCACGGAAAAACTACATTAATTGACTTTATTAGAAAAACTAAAGTTGCTGAAACTGAAAGTAGTGGTATTACTCAACATACTGGTGCTTACCAAGTAGTTTACAAAGGCAACAAAATTACATTTATTGACACTCCTGGACATGAAGCATTTACTGAAATGCGTTCACGTGGAGCTAAAATTACAGACATTATTATTTTAGTAGTAGCTGCTGATGATGGTGTTATGCCACAAACTAGAGAAGCTATTGACCACGCAAAAGCAGCTGATGTTCCTATCGTTGTTTTTGTAAATAAAATAGATAAACCAAATAAGGATCTTGAAAGAATTAAAAGAGAATTAGCTGAATGCAACGTTATTGTTGAAGAATATGGTGGTGACGTTCAAATGGTAACTGGTTCTGCTTTAAAAGGTCAAGGTATTGAAGATTTATTTGAAGCAATTAGTTTACTTGCTCAATTATTGGATTTAAAAGCCAACCGTTCAAGATATCCAGTTGGTACTGTTATTGAAAGTAAAATAGATAAAGGTGTTGGTGTTGTTTCAACTTTAATTGTTGAAAACGGTAGCTTATATAAAGGAGACTTTATTGTAGCTGGCTCAAGTTATGGGCGTGTGCGTTCATTAAAAGATGCTACTGGAAAACAAATGGAACATGCTCACCCTGGAACACCAGTTGTTGTTACTGGATTAAATAATTCACCACTTGCAGGTGATAGATTTATTGGTTTTCAAGATGAAAAATTTGCTAAAAAATTAGCTGAAGAAAAAGCTCAAATCGATAAGAACAGAGCTTTATATGACAAGAGCCAAACTTCAGAAATTGAAATTGGCAAAAAGGTATTTAATGTAATCATTAGATCAGATGTTCAAGGAACAGCTGAAGCTATCAAAGGCAAAATAGATGGCATGTGTAATGATGAAGCAATGATTAAAGTTATTGGCGCTCAAGCGGGACAAATAACAAATGCTGACTTATTATTAGCTCAAGCCTCAGATGCAAGGATTATAACTTTCAATGTTAAGCCATCTGCAGCTATTAAGCAAAGTGCAAAACATTCGGGAATTACAATTGTTAATTATTCTGTTATTTACAAAATTATTGAAGATATGGAAGCTCACCTTAAGGGTCAAAAAGCAGTTGTTTATGAAGAAAAGAAAATCGGATCAGCTCATATTATCAAAGTTTTCTTCTATTCAAAAGTTGGAGCAATTGCTGGATGTATGATGGATGAAGGCGTTGTAAAAGCTAATTGTAAAGTTAAAGTTTTTAGAGGCAGAAAAATGATTCATGATGGTGTAATTGAAACACTTAAACGTGAATTAAATGATTCAAAAGAAGTTGAGAAAGGTAAAGATTTTGGTACTCACATTAAGAAGTTTGATGATATCAAGGAAGATGATATTTTAGAATTCTTTGAAGATGTTCCAGTATCTTTATAA", "aa": "MKGKLYQINYVLDEEEIAEVCMERGLDFRKEQNIDAANFLHAVKFEDDEKSLVKRPPIITVMGHVDHGKTTLIDFIRKTKVAETESSGITQHTGAYQVVYKGNKITFIDTPGHEAFTEMRSRGAKITDIIILVVAADDGVMPQTREAIDHAKAADVPIVVFVNKIDKPNKDLERIKRELAECNVIVEEYGGDVQMVTGSALKGQGIEDLFEAISLLAQLLDLKANRSRYPVGTVIESKIDKGVGVVSTLIVENGSLYKGDFIVAGSSYGRVRSLKDATGKQMEHAHPGTPVVVTGLNNSPLAGDRFIGFQDEKFAKKLAEEKAQIDKNRALYDKSQTSEIEIGKKVFNVIIRSDVQGTAEAIKGKIDGMCNDEAMIKVIGAQAGQITNADLLLAQASDARIITFNVKPSAAIKQSAKHSGITIVNYSVIYKIIEDMEAHLKGQKAVVYEEKKIGSAHIIKVFFYSKVGAIAGCMMDEGVVKANCKVKVFRGRKMIHDGVIETLKRELNDSKEVEKGKDFGTHIKKFDDIKEDDILEFFEDVPVSL", "aa_hexdigest": "55a4826e0a8eda6a849cfc1cc2bdce66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8LI10", "query_cov": 1.0, "subject_cov": 0.9068219633943427, "identity": 1.0, "score": 1055.0, "evalue": 0.0, "valid": true, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "EFPKPENHBP_175", "locus": "GCA000186005_00135" }, { "type": "cds", "sequence": "contig_1", "start": 73394, "stop": 73909, "strand": "+", "frame": 2, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_013526633.1", "SO:0001217", "UniParc:UPI0000125CDD", "UniRef:UniRef100_Q9RFQ2", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_Q9RFQ2" ], "nt": "ATGGATTTAAAAAAATATATTCGAGATATTGAAAATTTTCCTAAAACAGGCATAATGTTTAAAGATATTTCACCATTATTGGCAAATGGTAAAGCATTAAATTATACAATCACAGAAATGGCAAAATTAGCTCAAGATGTTGATGTTATTGTTGGACCAGATGCGAGAGGATTTTTATTTGGCACGCCTACTGCAGCATTCTTGAAGAAACCATTTATTATGGTTAGAAAACCAGGAAAATTACCTGGTGATGTTATTTCAAAAAGTTACGATTTGGAATATGGAAATAATGTTTTACAAATTCAAAAAGGGTTTATTAAAAAAGGTCAAACAGTTGCAATTATTGATGATGTTTTAGCAACGGGTGGAACAACTAAAGCTATTATAAAACTATTAGAAGAACAAGGTGCAATTGTTAAAAAAGTTATTTTACTTCTTGAATTAGTAGATCTTAATGGTAGACAATTAATCACTAAAGATAATGATATTGAAATAGTTTCCTTAGTTAAATTTTAA", "aa": "MDLKKYIRDIENFPKTGIMFKDISPLLANGKALNYTITEMAKLAQDVDVIVGPDARGFLFGTPTAAFLKKPFIMVRKPGKLPGDVISKSYDLEYGNNVLQIQKGFIKKGQTVAIIDDVLATGGTTKAIIKLLEEQGAIVKKVILLLELVDLNGRQLITKDNDIEIVSLVKF", "aa_hexdigest": "4bb0073cef756d6de0022d2f5d20bfd0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000125CDD", "ncbi_nrp_id": "WP_013526633.1", "uniref100_id": "UniRef100_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CDD", "RefSeq:WP_013526633.1", "UniRef:UniRef100_Q9RFQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9RFQ2", "uniref90_id": "UniRef90_Q9RFQ2" }, "psc": { "uniref90_id": "UniRef90_Q9RFQ2", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "EFPKPENHBP_176", "locus": "GCA000186005_00136" }, { "type": "cds", "sequence": "contig_1", "start": 74239, "stop": 74424, "strand": "-", "frame": 1, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_UPI002805CAB8", "UniRef:UniRef90_UPI000F044D4B" ], "nt": "ATGGACTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTAATGAAGAAGAAACTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGA", "aa": "MDSNENFNGLVRRFFKKKTNFDDINEEETLKVQNEINNRPREIFGYLSALEMYEKLNAENP", "aa_hexdigest": "46b18a51e020676b557ee352632478bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 74188, "stop": 74628, "observations": { "mutation": [ 74241, 74550, 74583 ], "stop": [ 74241, 74550, 74583 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F044D4B", "query_cov": 1.0, "subject_cov": 0.4178082191780822, "identity": 0.9840000000000001, "score": 119.0, "evalue": 4.51e-33, "gene_start": 1, "gene_end": 61, "reference_start": 69, "reference_end": 129, "reference_sequence": "ISRYGFLVKIPNKNPWNLNLYLWNLIWKHKLNVKSITQDNGLEFSTLFFIGYKLKIFIYKADPYASFQMDSNENFNGLVRRFFKKKTNFDDINEEEILKVQNEINNRPREIFGYLSALEMYEKLNAENPWNSTGIDKVLYGKQKRN" }, "paralog": false, "identity": 0.958904109589041, "score": 283.0, "evalue": 2.68e-100, "description": "Internal stop codon at 74241, 74550, 74583. Nonsense mutation around 74241, 74550, 74583.", "psc": { "uniref90_id": "UniRef90_UPI000F044D4B", "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI002805CAB8", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F044D4B", "UniRef:UniRef50_UPI002805CAB8", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002805CAB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002805CAB8" ], "product": "IS30 family transposase" } }, "id": "EFPKPENHBP_177", "locus": "GCA000186005_00137" }, { "type": "cds", "sequence": "contig_1", "start": 75364, "stop": 75549, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGATAATCGAAAGCAGAATCGCAAATAATAAAATTACATTTTTCTTCAGTGGCATGAACAAAAAAGAATTTGAATCTTACTACCATGATCAACTTAAAAGAGAGCCTTACAAAGTTAAAACTTTTACTTCTCGAATTCTTAATAACACACTTGAATTTTGCATTGATGAAATTGAAAAATAA", "aa": "MEIIESRIANNKITFFFSGMNKKEFESYYHDQLKREPYKVKTFTSRILNNTLEFCIDEIEK", "aa_hexdigest": "af2a623284fc58496cd29cefca5b5ed6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.4203, "isoelectric_point": 6.563391685485839 }, "id": "EFPKPENHBP_178", "locus": "GCA000186005_00138" }, { "type": "cds", "sequence": "contig_1", "start": 75568, "stop": 75876, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF00308.24", "PFAM:PF01695.23" ], "nt": "ATGTTTGAATATATTAATCATCTAAAAGTTGCAATAGCTAAAAAGAAACAAAAAGGACTAAAAGGCATTTATGTCTATGGAGCTAACAACACTGGCAAAACTTACATTGCTAGTGCAATAGCCAACGAATTTGCAGCTGAAGGTATTTCATCAGTTTATTTAACAACCAGTGCTTTATATTCATTCTTAATTTCAAACATGGGTAGCAATGCTACAAATGCTAATGCAGTTATTGACATAATTAACAAACTCAAAAAAGTTAATGCATTAATTATTGACGAAATTGGTTTAGAAAAAAATAACTACTGA", "aa": "MFEYINHLKVAIAKKKQKGLKGIYVYGANNTGKTYIASAIANEFAAEGISSVYLTTSALYSFLISNMGSNATNANAVIDIINKLKKVNALIIDEIGLEKNNY", "aa_hexdigest": "cdc3287e94fa236ed8c6793449701b29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 73, "aa_cov": 0.7156862745098039, "hmm_cov": 0.9041095890410958, "evalue": 5.4548395620591484e-08, "score": 29.68245506286621, "start": 23, "stop": 95 }, { "id": "PF00308.24", "name": "Bac_DnaA", "length": 89, "aa_cov": 0.8529411764705882, "hmm_cov": 0.9438202247191011, "evalue": 9.864259308686022e-07, "score": 25.41262435913086, "start": 9, "stop": 95 }, { "id": "PF01695.23", "name": "IstB_IS21", "length": 76, "aa_cov": 0.7450980392156863, "hmm_cov": 0.9342105263157895, "evalue": 3.432052661071294e-08, "score": 29.561506271362305, "start": 23, "stop": 98 } ], "seq_stats": { "molecular_weight": 11094.677899999986, "isoelectric_point": 9.30196590423584 }, "id": "EFPKPENHBP_179", "locus": "GCA000186005_00139" }, { "type": "cds", "sequence": "contig_1", "start": 75952, "stop": 76257, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAAAAGAAGAAATTAAAAATGAGTTATTTTTTAATTCCAAGGCTGAATTTGAAAACAAGGCACTTGAAATAATGCATGGCCATCCGCAACTAGAAGCATTAATTCAAGCTAACAATATTACAAATGAAGAAATTTTGGAAAACTTATTTACATTTATTAATATTAAAGAATCATTGGATAATTCTCAAATTTATCCATGGATTTTTTCTATTTCAAGAAAAAATGAAAAATTAGTTTTTAAAAAGATTGCTGCAAAAAATGAATACAAATCAGTTGTTACACGACACATCAATTTATGA", "aa": "MAAKEEIKNELFFNSKAEFENKALEIMHGHPQLEALIQANNITNEEILENLFTFINIKESLDNSQIYPWIFSISRKNEKLVFKKIAAKNEYKSVVTRHINL", "aa_hexdigest": "389e0559edf23678371fb16b54db587f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11823.435999999983, "isoelectric_point": 6.295850563049316 }, "id": "EFPKPENHBP_180", "locus": "GCA000186005_00140" }, { "type": "cds", "sequence": "contig_1", "start": 76271, "stop": 77233, "strand": "-", "frame": 3, "gene": "dnaD", "product": "DnaD domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526636.1", "SO:0001217", "UniParc:UPI0001A8C3A8", "UniRef:UniRef100_C4XE27", "UniRef:UniRef50_N9V249", "UniRef:UniRef90_C4XE27" ], "nt": "ATGCTAAAAAATATTCCTTACGCATTCTTCAGAATCAAACATTCTTCATCAGTTAGTGGAGAAGATTTAAAAAACTTACGCAAGTTTTACGCTCCTATTTTAGGTAGTGATGCAATTTTGCTTTATGAATACCTTAGAGATCTATCATTTTCTCAATCTCCAGAAACTGGTTTTTATGACTATGATAGCCTTACTTATCTATTAAATATGGATGTAAATAAACTAAATCAAGCACGTATAAAATTAGAATCTGTATCTTTAATTTCTACTTTTATAGATGAGTTTAATCGCAAAACACTTTTTGTTATTGAAAAACCACTTGATAATACAGGTTTCAAAAAGAATTTAATTCTAGCAAACAAACTCATTAGTATTATTGGTAGCAAAAACTTTGAAAGATTAGTTGGACGTGAAAGAAATATTTATTTAAGTAAAGCTTCAAATCTTTTGGATGCTTCAGCTTCTTTTGAAGAAGTTTTTCCTGAACCAAGTGAAGAATTAACTATTACTTTTTCACATGAGGTTGACTCAAGTATGATTAAAACTCAAGAAATAGTTGAAGAAATTCAAACCAAATTGTCACTTAATACTTTTGATTATCCTAATCCTTATGAAGCAATACTTAAAACTGATTCAAGATACTTCTATTCTCAAATTAGTCACAAAATACCACGTCAAAGTGTTTTAGATTTAATTAAAGAAAGTAGAGCAGCTGGCTTAGGTGATCCTTGCTTAAACTTAGTATTCTTTTATTCAAATGAAATAAATAATTGCATAAATACCAATTATGTGGGTAAAATTATTAAGGATTTAATTAAAAAAGAAATCTTCTCATTTGAAGCAATTGAAAATTATTTAGATGACATACTTAAAGTTAAAAACAGAATTGCAGTTTCAAAAATTAATTTATATAAAGCTACCTATTTAGAATCACTTCAAAGAGAAGACAACAACAATATTTAA", "aa": "MLKNIPYAFFRIKHSSSVSGEDLKNLRKFYAPILGSDAILLYEYLRDLSFSQSPETGFYDYDSLTYLLNMDVNKLNQARIKLESVSLISTFIDEFNRKTLFVIEKPLDNTGFKKNLILANKLISIIGSKNFERLVGRERNIYLSKASNLLDASASFEEVFPEPSEELTITFSHEVDSSMIKTQEIVEEIQTKLSLNTFDYPNPYEAILKTDSRYFYSQISHKIPRQSVLDLIKESRAAGLGDPCLNLVFFYSNEINNCINTNYVGKIIKDLIKKEIFSFEAIENYLDDILKVKNRIAVSKINLYKATYLESLQREDNNNI", "aa_hexdigest": "baca658575854f61784cbbd66cb31001", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C3A8", "ncbi_nrp_id": "WP_013526636.1", "uniref100_id": "UniRef100_C4XE27", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3A8", "RefSeq:WP_013526636.1", "UniRef:UniRef100_C4XE27" ] }, "ips": { "uniref100_id": "UniRef100_C4XE27", "uniref90_id": "UniRef90_C4XE27" }, "psc": { "uniref90_id": "UniRef90_C4XE27", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_N9V249" }, "pscc": { "uniref50_id": "UniRef50_N9V249", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V249" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaD" ], "id": "EFPKPENHBP_181", "locus": "GCA000186005_00141" }, { "type": "cds", "sequence": "contig_1", "start": 77283, "stop": 77627, "strand": "-", "frame": 2, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_A0A3P8KM71", "UniRef:UniRef90_C4XE26" ], "nt": "TTGGGAGAATTGGAAAAAATCATTTATCCTTTAATTTTTGAAGAAATAAAAGATGGAAATTTTTTCATTGTTGAGATACCAAATTTATTGCCAAAAAATTTTAAATTTATTTCTTTGTTTTCTGCAGTTTTGTGCTTAGAAACTAGCCAAAAAAATAGGTTAAAAAATTTTAAAAAACGTAATGTGGATAAAACTAAAATTAAGGCAATAGATGAAAAAAACAACCCTTTTTCGATAAAAAATCAACTTTTTGGGTCTATACCAATTGTGGATTTCTATGGGCATAACTTCACTAATAATGAAAATATTTTAGATTTTTTAAAACTTCTATTTTTTATCTCATAA", "aa": "MGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNNPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS", "aa_hexdigest": "ded5c8f640009da2b7fb50f435665ff9", "start_type": "TTG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "3-prime", "pseudogene": { "start": 77283, "stop": 77855, "observations": { "mutation": [ 77651 ], "stop": [ 77651 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE26", "query_cov": 1.0, "subject_cov": 0.6, "identity": 0.991, "score": 224.0, "evalue": 3.29e-73, "gene_start": 1, "gene_end": 114, "reference_start": 77, "reference_end": 190, "reference_sequence": "MIALIGQIAVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKLWIDQKAHNLGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNNPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS" }, "paralog": false, "identity": 0.9947368421052631, "score": 379.0, "evalue": 1.17e-136, "description": "Internal stop codon at 77651. Nonsense mutation around 77651.", "psc": { "uniref90_id": "UniRef90_C4XE26", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_A0A3P8KM71", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE26", "EC:2.7.1.24", "UniRef:UniRef50_A0A3P8KM71", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM71" ], "product": "Dephospho-CoA kinase" } }, "id": "EFPKPENHBP_182", "locus": "GCA000186005_00142" }, { "type": "cds", "sequence": "contig_1", "start": 77649, "stop": 77855, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTAATTGGTCAAATTGCCGTGGGAAAGAGTACTTTTTTAGAAAATTTTAAGAAATTAGGTCTTAAAACTTTTAATTGTGACGAGTTTATTAATCGTGAATATCAAAAAAATGGCAAAATTTACCACAAAATTAATCAAGAATTAGGTGATTTTTTAAATGATAAAAATGGTATAAGTAAGGAAAAAATTAAACTTTGA", "aa": "MIALIGQIAVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKL", "aa_hexdigest": "efc83422adb763fcb088466d62bf4452", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7849.048899999999, "isoelectric_point": 9.336520957946778 }, "id": "EFPKPENHBP_183", "locus": "GCA000186005_00143" }, { "type": "cds", "sequence": "contig_1", "start": 77857, "stop": 78231, "strand": "-", "frame": 1, "gene": null, "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354345.1", "SO:0001217", "UniParc:UPI0001E32F4A", "UniRef:UniRef100_C4XE25", "UniRef:UniRef50_D5E655", "UniRef:UniRef90_C4XE25" ], "nt": "ATGGAAAAAGCACTTTTATATGAAATCAAAAAATCAAAGTTTTATTCTTTAGCTTTTAATGTGACTAGTAAGGAACAAATAAAAGAAGTAGAAGCTAATTTAAGAAAAGAATACAAGAAAGCAACTCATATTTGTCATGGTTATAGCTTTTTAAATAATGGAGTGATCAATGCAGGCTTTAGCGATGATGGCGAACCTAATGGTACAGCCGGCAGACCAATTAGAGATCTTTTAATTATGCGCAAAATTAATAATGTTGTTGTTTTTGTAATTCGCTATTTTGGTGGAATTAAGTTGGGAGCCAACGGTTTAGCTAGAGCTTATATTAAAAGTGCAAACTTAGCTATAGAAGAATATATAAAGGAGGGAGCCTAA", "aa": "MEKALLYEIKKSKFYSLAFNVTSKEQIKEVEANLRKEYKKATHICHGYSFLNNGVINAGFSDDGEPNGTAGRPIRDLLIMRKINNVVVFVIRYFGGIKLGANGLARAYIKSANLAIEEYIKEGA", "aa_hexdigest": "28f4398ba41d5b95b867a2e497c60f17", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F4A", "ncbi_nrp_id": "WP_013354345.1", "uniref100_id": "UniRef100_C4XE25", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F4A", "RefSeq:WP_013354345.1", "UniRef:UniRef100_C4XE25" ] }, "ips": { "uniref100_id": "UniRef100_C4XE25", "uniref90_id": "UniRef90_C4XE25" }, "psc": { "uniref90_id": "UniRef90_C4XE25", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D5E655" }, "pscc": { "uniref50_id": "UniRef50_D5E655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E655" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_184", "locus": "GCA000186005_00144" }, { "type": "cds", "sequence": "contig_1", "start": 78298, "stop": 78744, "strand": "-", "frame": 1, "gene": null, "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "RefSeq:WP_013526638.1", "SO:0001217", "UniParc:UPI0001F59BD7", "UniRef:UniRef100_C4XE24", "UniRef:UniRef50_A0A449BAM0", "UniRef:UniRef90_C4XE24" ], "nt": "ATGGCTAAAAAAAATATTATTGCTCTTGCTAGCGACCATGGTGGTTGCGAATTAAAAAATGAAATGCGCGATTATGTAAAAAGTTTAGGTTATGAAGTTGTTGACTTAGGACCTGCGGATGGCTCAAAAAGTATTTCATATGCTGAACAAGGGCATAACCTAGCTAAATATATTGATGAACATAAAAACACAATTGGTTTAGGTTTTTGTGGTACAGGTTTAGGTATTTCATATGCCTTAAACCGTCATAAACACATTCGCGCAGCTAGAGTAGTTACAGTTGAAGATGCAAGGCTTGCTAAATTACATAATAATGCTAATGTTTTAGTTATGGGTGGTAGATATGTACCTTTTGCTGAAGCACAAGCTATGTTTAACGAATACATTAAAACTGAATATGAAGGTGGCAGACACCAAGCAAGAATCGATCAAATCGATGAATTCTAA", "aa": "MAKKNIIALASDHGGCELKNEMRDYVKSLGYEVVDLGPADGSKSISYAEQGHNLAKYIDEHKNTIGLGFCGTGLGISYALNRHKHIRAARVVTVEDARLAKLHNNANVLVMGGRYVPFAEAQAMFNEYIKTEYEGGRHQARIDQIDEF", "aa_hexdigest": "4f5c7f5bd1fa86bfcb99770a47fce7af", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59BD7", "ncbi_nrp_id": "WP_013526638.1", "uniref100_id": "UniRef100_C4XE24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BD7", "RefSeq:WP_013526638.1", "UniRef:UniRef100_C4XE24" ] }, "ips": { "uniref100_id": "UniRef100_C4XE24", "uniref90_id": "UniRef90_C4XE24" }, "psc": { "uniref90_id": "UniRef90_C4XE24", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_A0A449BAM0" }, "pscc": { "uniref50_id": "UniRef50_A0A449BAM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAM0" ], "product": "Ribose-5-phosphate isomerase B" }, "genes": [], "id": "EFPKPENHBP_185", "locus": "GCA000186005_00145" }, { "type": "cds", "sequence": "contig_1", "start": 78877, "stop": 79503, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAAAAAAACAAGAAGATATAAAAGACTGTGGTTTATATGTTTTGCAGTATTTTGTGGCAAAACATAATGATGACTACATTGATATTAATTATTTTAAAAATAAGGCAACTTACTCTTCAGAAGGAGTAAATATTTCAACACTTAAAAAATTAGCTTCTGAACACGATATCGATTTAAATAGTTATACAGGTGATTTTGAATCTCTTGAATCTTTGGACAATTCAAATATGCCTATAGCTTTATTATTAAATAGAAAAGGATATAGCCATTATGTAATTTTAGAAAAAATGAAGGACAATTATTTTATTGTTCAAGATCCAGCTTATGGTCGCAAGATGAAAGTTAAAAAGAAAGACTTGGCTAAGGAATTTGCCAATGTGATACTATTTTTTGATCCAAAAGAAGAAAAATCAAAAAATAAAAATAAAAACTATTCACTTGATAATAAAATAAGCACATTATTTTCATTTAAAAAATATACTTATCCTTTATTCATTTCAGCACTTTTAGGATTGTTTTTATCATTTGGATCAACATTTTTTATAAAAATAGTTTTTGATTTTATATTGCCTAATTATCTTAAAAATAATTTGATTATTATTTTTGTATTTTTCCTATGA", "aa": "MRIKKQEDIKDCGLYVLQYFVAKHNDDYIDINYFKNKATYSSEGVNISTLKKLASEHDIDLNSYTGDFESLESLDNSNMPIALLLNRKGYSHYVILEKMKDNYFIVQDPAYGRKMKVKKKDLAKEFANVILFFDPKEEKSKNKNKNYSLDNKISTLFSFKKYTYPLFISALLGLFLSFGSTFFIKIVFDFILPNYLKNNLIIIFVFFL", "aa_hexdigest": "54bc59a349a4f6b16e512665ed051fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24377.981800000027, "isoelectric_point": 9.086319160461429 }, "id": "EFPKPENHBP_186", "locus": "GCA000186005_00146" }, { "type": "cds", "sequence": "contig_1", "start": 79516, "stop": 79767, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAATCGGTTGTTTAAAAATCATGTTATTAAAAAAATAAAAAACGATTTAGAGTTTACTTTAACAAAAGCATTTTTTGACAAAATAAAAAGAGTGCCATCAAATTATTTATCTAAATTAACAAGCAATGATTACTTTAAAAGACTTACTTATATAAAAGAAATATGTGAATTTCAATCTAATTTTGTCTTTACTTTTTTAAGTGAAATATTTTCTTTTATAGGTTCAACAATCTTATTGATTTGA", "aa": "MFINRLFKNHVIKKIKNDLEFTLTKAFFDKIKRVPSNYLSKLTSNDYFKRLTYIKEICEFQSNFVFTFLSEIFSFIGSTILLI", "aa_hexdigest": "ada8443b97f9896a4645d090619f7959", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9958.638099999993, "isoelectric_point": 9.673174858093262 }, "id": "EFPKPENHBP_187", "locus": "GCA000186005_00147" }, { "type": "cds", "sequence": "contig_1", "start": 79930, "stop": 80043, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTAGATTATTCAAGATTTATTTCTAAAAACCAATTTAAAAACATGTTGGAATTTAAGAAAAAAGAATATTCATTTGCTTCTAAAGATGATATTAAAAACTTTTGA", "aa": "MDNVDYSRFISKNQFKNMLEFKKKEYSFASKDDIKNF", "aa_hexdigest": "5e6c384aeaedc959b54a9a48799bcbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4557.1226000000015, "isoelectric_point": 9.100179862976074 }, "id": "EFPKPENHBP_188", "locus": "GCA000186005_00148" }, { "type": "cds", "sequence": "contig_1", "start": 80134, "stop": 80940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATGTTCTTAAATGGAATAAATTTCTTTGTTAATCCAATGCTAAATATGAGCTCATTATTACTTTCTCAAACAATGATCAAAAGAAATGTTAATTTAGTAAATTTTGTTTTAAATTTAGACGAAAAAAGCAATTTAGATACTGGTTTATTCATCGATAAAATTAAAAAAATTGAATTAGAAAATGTTAATTTTTATTACGAAAACGGAAAAAATATTTTAAATATTCCTTCATTTACGATTGACCAAAATATTCAATTAAATGGTAAAAATGGTAGTGGTAAAACCACTATGCTAAGTTTTATAAACGGCTGTTTTAAAGGTTATTTAGGAAAAATGAAGATTAATGATATTGATTTTGATTCAATAAATTTGATTAATTATCAAAGTAAAAATATATTGATAAATTCTTCCAGCCATTTACCAGATGTTTCTTTTCTCGAATATATTACAAGTAACAATGAAAATTATAAAAATGAATTGAATAATAATATTCAAAAATATGATCTATACAAAATATTTGAAGAAATGAATTTTAATTTAAATGGCAGCATAATTAGTAATGGAAGCAATTTGTCAAGTGGACAAAAACAACTTTTATTATTACTAAAATTATTTGTAAGAAAATATGACTTAATCATGCTTGATGAAGCCTTTGAAAATATTAATCCAAGTAATTGTGAATTATTGAAAAAGGCTATAAAGAAATATCAAGATGAAGCATTATTTATTGAAATAAGTCACAATAGGAACTATTTATTTAAAACAAAGGAGGTGTGCTTTGAAAAAATTAACAACTGTTTCTAA", "aa": "MMFLNGINFFVNPMLNMSSLLLSQTMIKRNVNLVNFVLNLDEKSNLDTGLFIDKIKKIELENVNFYYENGKNILNIPSFTIDQNIQLNGKNGSGKTTMLSFINGCFKGYLGKMKINDIDFDSINLINYQSKNILINSSSHLPDVSFLEYITSNNENYKNELNNNIQKYDLYKIFEEMNFNLNGSIISNGSNLSSGQKQLLLLLKLFVRKYDLIMLDEAFENINPSNCELLKKAIKKYQDEALFIEISHNRNYLFKTKEVCFEKINNCF", "aa_hexdigest": "61d6e27bcc3f2d7163edc3a59f445cf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 140, "aa_cov": 0.5149253731343284, "hmm_cov": 0.9, "evalue": 8.299257539976334e-18, "score": 61.646934509277344, "start": 82, "stop": 219 } ], "seq_stats": { "molecular_weight": 31045.39700000003, "isoelectric_point": 6.744537544250489 }, "id": "EFPKPENHBP_189", "locus": "GCA000186005_00149" }, { "type": "cds", "sequence": "contig_1", "start": 80963, "stop": 81301, "strand": "+", "frame": 2, "gene": null, "product": "MAG1140 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05", "UniRef:UniRef90_A0AB32XAW0" ], "nt": "TTGTTAGCTATTGCAACAGTAACTTTAATTGTTTTTATTTGTTTATATCATGTTGACAAAACAATAAGAGTTAAGTTACAAGTTAATGACAAAAAAGACTTAACTTTGTTAGCAAATGATAAGACTGCTTATTTATTAAAGAAAAATCAAAAAATAATAATTAATGTAAATGACCAAATTTTCAATTTAAAAATCCGCAACTTAAAAATTGAAAATCAAACGATTAAAGTAACCTTCTATAATTTGCCTAATAGTTTGAAACTTTTACCTAATACAACTTTAGATGCCACGATATTTGTGGATCGAGCCACTCTATTAAATTTAATATTAAATTCATAA", "aa": "MLAIATVTLIVFICLYHVDKTIRVKLQVNDKKDLTLLANDKTAYLLKKNQKIIINVNDQIFNLKIRNLKIENQTIKVTFYNLPNSLKLLPNTTLDATIFVDRATLLNLILNS", "aa_hexdigest": "4928e7b648ebfbc2b2b36daba65916e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XAW0", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.991, "score": 202.0, "evalue": 1.92e-65, "valid": true, "product": "MAG1140 family protein", "uniref50_id": "UniRef50_A0A3P8LI05" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05" ] }, "genes": [], "id": "EFPKPENHBP_190", "locus": "GCA000186005_00150" }, { "type": "cds", "sequence": "contig_1", "start": 81322, "stop": 81540, "strand": "+", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "GO:0004540", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_L0RUR5", "UniRef:UniRef90_C4XE22" ], "nt": "ATGGTTGAATTAAATGTTGTAAACAAGACTAAAAATAAATTTTTGTTTGAAAAAGAATACAAGCAAATATTAGAAAATTTAGCTAAAGAATTTGGGCTTAATCAAACTGTTTCAGTTGATGTAACTATTACAAATAATGATGAAATCCAAAAACTGAATAAAGAATATAGAAACAAAGATTATCCAACAGATATCTTGTCTTTTGGTTTAGAAGATTGA", "aa": "MVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLED", "aa_hexdigest": "9720586fa9bf70882e50697d09f9bd76", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "3-prime", "pseudogene": { "start": 81256, "stop": 81777, "observations": { "mutation": [ 81538 ], "stop": [ 81538 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE22", "query_cov": 1.0, "subject_cov": 0.4161849710982659, "identity": 1.0, "score": 141.0, "evalue": 2.43e-41, "gene_start": 1, "gene_end": 72, "reference_start": 23, "reference_end": 94, "reference_sequence": "MCGSSHSIKFNIKFIINNIIRIMVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLEDWELFENMPIYPLGELVISHEKVEAQAQEFVHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE" }, "paralog": false, "identity": 0.9826589595375722, "score": 340.0, "evalue": 3.09e-122, "description": "Internal stop codon at 81538. Nonsense mutation around 81538.", "psc": { "uniref90_id": "UniRef90_C4XE22", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_L0RUR5", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE22", "UniRef:UniRef50_L0RUR5", "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUR5" ], "product": "Endoribonuclease YbeY" } }, "id": "EFPKPENHBP_191", "locus": "GCA000186005_00151" }, { "type": "cds", "sequence": "contig_1", "start": 81556, "stop": 81777, "strand": "+", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "nt": "ATGCCAATTTATCCCTTAGGTGAATTAGTAATAAGCCATGAAAAAGTTGAAGCACAAGCTCAAGAGTTTGGTCATTCAATTCGTAGAGAATATTGTTATTTATTTGCTCATGGATTAATTCACTTGATGGGATATGATCATGAAACAGAAGTCGAAAGAGCTGAAATGAATAAACTTGTTGATTTAATATTTGAACCATTAAAAATAAGTAGAGAGGAATAA", "aa": "MPIYPLGELVISHEKVEAQAQEFGHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE", "aa_hexdigest": "4069d2eaaa4daa1d06dcff116782426f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024C77EC7", "query_cov": 0.8904109589041096, "subject_cov": 1.0, "identity": 0.631, "score": 90.9, "evalue": 9.81e-23, "valid": false, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_UPI0024C77EC7", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024C77EC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "product": "rRNA maturation RNase YbeY" }, "genes": [ "ybeY" ], "id": "EFPKPENHBP_192", "locus": "GCA000186005_00152" }, { "type": "cds", "sequence": "contig_1", "start": 82051, "stop": 82173, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAATTTATGCCCGATGATGGTTTAATTTACCAATACAATAAAAATGGCGACAAATGTAGAGTAAATAAAGTAATTGAAATGGTCCCATTTCCTATTAGAACAGAGCAAATTAAATAA", "aa": "MTEFMPDDGLIYQYNKNGDKCRVNKVIEMVPFPIRTEQIK", "aa_hexdigest": "2888be777c1f96d3b037da4c5c27f0e2", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4762.5082, "isoelectric_point": 6.058149909973144 }, "id": "EFPKPENHBP_193", "locus": "GCA000186005_00153" }, { "type": "cds", "sequence": "contig_1", "start": 82173, "stop": 83000, "strand": "+", "frame": 3, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q98QI1", "UniRef:UniRef90_C4XE20" ], "nt": "ATGAAAGTATGTTTAGCTAGTATTATCGGCAGACCTAATGTAGGAAAATCATCATTAATAAATCAAATTGTTAAATATGATGTTGCAATTGTAACTAATGTTCCGCAAACAACAAGAGATCAAATTGTCGGCGTTTATAACGAAGATGATTTTCAATTAGTTTTTGTTGATACACCAGGTATCCACAAACCATTAAATTTATTAGGTGAAGCCTTAAATAAAAGTGCTTTTGAATCTACAAAAGATGTTGATTGTTTAATATTTCTTTCTCCTGTTAATGAAAAAGTTGGACAAGGAGATATTTTAATTTTAGATAAGATCAAAAATACCAAGAATAAAATTGCAGTCATTTCTAAAATTGATTTAGCCAAAAAACCAGAAGAAATACAAGAAAAAATTGAAGAATTAAGCAAATACAGTTTTGACAAAATATTGTCTGTTTCAAGAAATAATGAAAACTCAATTGAAGAATTAATTAAAGTTATTAAGCAATATGCCTATGAAGGTGAGCCATTTTATGATGAAGACTATATAACAGATAAATCAATGAGATTTTTAGCAAAAGAGATAATAAGAGAAAGTGCGATTAATCTTTTAAGAGAAGAGTTGCCACACTCAATTGCTGTAGAAGTTATTGATTTTATTGAAGAAGATGATATTAAAATAAATGCCATCATTTATGTCAAAAAAGCTTCTCAAAAAGGAATGGTAATTGGCAAAAATGGTCAAATGATAAAACAAATAGGAACCAACGCAAGAAAGAAAATGATGAGTCAATTTGATTCAAAAGTGGAATTAAATTTAAAAGTTAAAATAGCAAACAAATGA", "aa": "MKVCLASIIGRPNVGKSSLINQIVKYDVAIVTNVPQTTRDQIVGVYNEDDFQLVFVDTPGIHKPLNLLGEALNKSAFESTKDVDCLIFLSPVNEKVGQGDILILDKIKNTKNKIAVISKIDLAKKPEEIQEKIEELSKYSFDKILSVSRNNENSIEELIKVIKQYAYEGEPFYDEDYITDKSMRFLAKEIIRESAINLLREELPHSIAVEVIDFIEEDDIKINAIIYVKKASQKGMVIGKNGQMIKQIGTNARKKMMSQFDSKVELNLKVKIANK", "aa_hexdigest": "fd3af8ade559fc7fb09080e965318b26", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XE20", "query_cov": 1.0, "subject_cov": 0.9482758620689655, "identity": 1.0, "score": 518.0, "evalue": 3.06e-185, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_Q98QI1", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QI1" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "EFPKPENHBP_194", "locus": "GCA000186005_00154" }, { "type": "cds", "sequence": "contig_1", "start": 83531, "stop": 84124, "strand": "+", "frame": 2, "gene": null, "product": "M24 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "nt": "ATGACAGAAAAAGAAGTAGCTGCATACTTAAATTATTTAATGAAAAAACATGGATCAGATAAAGAAAGTTTTGATGAAATAGTTGCTACAGGGCCTTCATCAGCTGAACCTCACCACCATCCAACAGATAGAAAATTAAAAGAAGGGGATCTTCTTAAAATCGACTTTGGTGCTCTTTACAAAGGATACAGTGCAGATATTACTAGAACATGTATTTTAGGTGGAGACAAAAAAGCGAATGATCCTAAACAATTAGAAATTTTACAAATAGTTATGGAAGCTGCAAAAGCTGGTCGTGATGCAGTAAGACCTGGAATTAAAGCAAGTGAAATTGATAAAATTTGTCGTGATTACATAACTAAAAAAGGATATGGTAAATACTTTGTTCACTCAACTGGTCATGGACTTGGAATTGATGTACATGAATTACCTAATGTTAGATCAACAAGTGACTATATTTTAGAACCTGGAATGATAATTACTGTTGAACCTGGTATTTATATTGAAGGTTTAGGCGGAGCAAGAAATGAAGATGATGTTCTTGTTACAGAAAAAGGTCGTTATGTATTCAGTAGACCTGAAGAAAAATCTTAG", "aa": "MTEKEVAAYLNYLMKKHGSDKESFDEIVATGPSSAEPHHHPTDRKLKEGDLLKIDFGALYKGYSADITRTCILGGDKKANDPKQLEILQIVMEAAKAGRDAVRPGIKASEIDKICRDYITKKGYGKYFVHSTGHGLGIDVHELPNVRSTSDYILEPGMIITVEPGIYIEGLGGARNEDDVLVTEKGRYVFSRPEEKS", "aa_hexdigest": "bbf05903b3e9a818f78b4c01a085a7bf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00313C6812", "query_cov": 0.8883248730964467, "subject_cov": 0.8663366336633663, "identity": 0.514, "score": 177.0, "evalue": 4.17e-53, "valid": false, "product": "M24 family metallopeptidase", "uniref50_id": "UniRef50_UPI00313C6812" }, "pscc": { "uniref50_id": "UniRef50_UPI00313C6812", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "product": "M24 family metallopeptidase" }, "genes": [], "id": "EFPKPENHBP_195", "locus": "GCA000186005_00155" }, { "type": "cds", "sequence": "contig_1", "start": 84198, "stop": 84569, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01938.26" ], "nt": "ATGAAATATGAATTAGGCCAAATAATAAAAGGAGTGGAAGCAATAAATTTTACTTATGAAGCATTAGGCTTAGTTAAAGCAGATGATTATTCTATTTTTGTTGAGAATTTATTGCCACAAGAAAAAGCAGATATACAAATTAAAAAATCGAACACAAAATTTGCTTTTGCTATAGTTGTAAAAAGATATAACGATAGTCCAAAAAGAATAAAAGTTTTAAATCAAAAATTAATGCTAAGTGGTTCAACTCCATTAGCAATTTTAAACTATGCAGATCAATTAGAATTTAAAGAAAATGTTGTTAAATATTTATTTCAAAGAAATATCCATTTTAATGAAACACAAAAAATTTTGCCGTGTGAAAAACAATGA", "aa": "MKYELGQIIKGVEAINFTYEALGLVKADDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQ", "aa_hexdigest": "b300418954365ea01cdf738fe394eb96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01938.26", "name": "TRAM", "length": 40, "aa_cov": 0.3089430894308943, "hmm_cov": 0.975, "evalue": 5.109556491755421e-07, "score": 25.837770462036133, "start": 23, "stop": 60 } ], "seq_stats": { "molecular_weight": 14244.428599999983, "isoelectric_point": 9.118231010437015 }, "id": "EFPKPENHBP_196", "locus": "GCA000186005_00156" }, { "type": "cds", "sequence": "contig_1", "start": 84708, "stop": 85514, "strand": "+", "frame": 3, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_C4XE18", "UniRef:UniRef90_C4XE18" ], "nt": "ATGTATTTAAACAAAAATATTAACAATTACAAATGTTTTATAGAAAACGGACAATTTTTAAAACAAATTGTTGTTAGATATTCAGAATCATATGATCAAATGATGTTAGCTTTTGTTCAAGACAAACCATTTGAATTATCTGAAGATTTATTAAAAGATTTAACCTCAAATTTTAAAAATTTAAAAGTTATATTAAAAGAAGAAAAAGTTAAAAAAACAACTTATACTTATTATTCAGAAGCTAAAAACATACAAGACAAAATTAGCAATTTAACTTTTAATATTGATTACAATAGTTTCTTTCAAGTTAACTCGTATCAAACCAATAATTTATATAACTTGCTAGTTAATAATTTGCACCTTGATAAAACTATGAATGTAGTTGATGCATATTCTGGAATCGGAACAATAAGTTTAAAAATTGCAGAAAAAGTTAAAAAAGTTTATGGACTTGAAATAGTAAAAGAAGCAGTTATAAATGCTAAGCAAAATGCAAAATTAAATAACATAAATAATACAGAATTTTATGAAGGCGATGTTTTAAAAACTATTGATAACATTAAAGAAAAAATAGACACAATAATAATAGATCCTCCAAGAGCAGGAGTATCGAAAGAAGTTTTAGAAAAAATATTAAAAATTGAACCAAAACAAATTGGTTACATAAGTTGTAATCCTCACACACTTTGCAGAGATATAGATTTATTAACATTATCAAAAAAATACAAATTAGAATTTTTAAAACCATGTGATATGTTTTGTCAAACACACCATGTAGAAACAGTTGCAATTTTAAAATTAAAATAA", "aa": "MYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVNNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNTEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVETVAILKLK", "aa_hexdigest": "53bd3085092ef572d0638fc831bfa033", "start_type": "ATG", "rbs_motif": null, "genes": [ "rlmD" ], "truncated": "5-prime", "pseudogene": { "start": 84408, "stop": 85514, "observations": { "mutation": [ 84567 ], "stop": [ 84567 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE18", "query_cov": 1.0, "subject_cov": 0.6077097505668935, "identity": 1.0, "score": 525.0, "evalue": 1.37e-185, "gene_start": 1, "gene_end": 268, "reference_start": 174, "reference_end": 441, "reference_sequence": "MRNMKYELGQIIKGVEAINFTYEALGLVKADDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQWNYRNKITVFVESSKGKIKFGLYEKNTHKLVEQDSYDLANESINKLLMYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVNNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNTEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVETVAILKLK" }, "paralog": false, "identity": 0.9972826086956522, "score": 729.0, "evalue": 2.65e-268, "description": "Internal stop codon at 84567. Nonsense mutation around 84567.", "psc": { "uniref90_id": "UniRef90_C4XE18", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_C4XE18", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE18", "EC:2.1.1.190", "UniRef:UniRef50_C4XE18", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE18" ], "product": "TRAM domain-containing protein" } }, "id": "EFPKPENHBP_197", "locus": "GCA000186005_00157" }, { "type": "cds", "sequence": "contig_1", "start": 85572, "stop": 85706, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BDB", "UniRef:UniRef100_A0AB32XAH3", "UniRef:UniRef50_A0AB32XAH3", "UniRef:UniRef90_A0AB32XAH3" ], "nt": "ATGGAATTAGAATTTCTAGAATTTAATTATTTATTTAAGTCCCCTATTCAAATAGTAAATTATTATTCTATGAAAAAAATGATTAAAGTTGCAAAACTAAATTCAGAATTATTAAAACTTTTGGTATGCAATTAG", "aa": "MELEFLEFNYLFKSPIQIVNYYSMKKMIKVAKLNSELLKLLVCN", "aa_hexdigest": "2cd743ec5c70526871689779ff580ecb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BDB", "uniref100_id": "UniRef100_A0AB32XAH3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BDB", "UniRef:UniRef100_A0AB32XAH3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAH3", "uniref90_id": "UniRef90_A0AB32XAH3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAH3", "uniref50_id": "UniRef50_A0AB32XAH3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XAH3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAH3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5287.3682, "isoelectric_point": 8.85242786407471 }, "id": "EFPKPENHBP_198", "locus": "GCA000186005_00158" }, { "type": "cds", "sequence": "contig_1", "start": 85944, "stop": 86420, "strand": "+", "frame": 3, "gene": null, "product": "DUF3137 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16", "UniRef:UniRef90_C4XE16" ], "nt": "ATGGGAGTTACAACTAAACCAAAGAGTTATTTAGATTTTGTGAATGAAAACTTACAATCATTTAAAGAAATAGTTTCAAACAGTTTTGATTCAGTTATTAAAAATAAAAAGAATATTTTGTCAAAATTAAAAATATTACTAATTGTTTCAATAGTACTATTAGTTTTAGGTGGAATTGGATTAATATTATCTTTCTTTTTCTTCATAAAAACTAATTCAAAAAATACAGCAATATTTGCTGGATTAATTATTTCTGCAATAGTAACAGGAATTGGTTCAATTGTATTATCAGTATTTTTAGGAAAAAGGAAAAAACTTTTTAAAGAACTCAAACACGTTCTTGATAAAGAAAAATTATTTGACAAAGCTTTTGAAAAATTATCATACAAAGCAATCGATCCAAATGACCCAGAACAAGCACAATTATTAGAAAAATTTAAACAAGGCTATGATAAAAATAGAGATTGTTTAGACTGA", "aa": "MGVTTKPKSYLDFVNENLQSFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKQGYDKNRDCLD", "aa_hexdigest": "08c5df4cc96faed715c139dffb82d168", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 85944, "stop": 86723, "observations": { "mutation": [ 86418, 86445, 86517, 86544 ], "stop": [ 86418, 86445, 86517, 86544 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE16", "query_cov": 1.0, "subject_cov": 0.4328767123287671, "identity": 1.0, "score": 290.0, "evalue": 8.36e-96, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MGVTTKPKSYLDFVNENLQSFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKQGYDKNRDCLDWVDHIPGAYWIPSDANVDSTGKIYFVMDKNENPWVIQTVRYHWVREERDLKGNITIRHIYDYATLFEGIFYSLPKSKNFTLAIGNRCGLTNNLKQNIKYENQEFDKLFKAYCSDSLKGHNIYQPYPMEITLEHCKQNKSYVKSFGMLLDDQNIKCWVRPRGSILEIDMPSNTINKDKIVKTVCKDILEDTYSVYWLFSLLTIPGYFN" }, "paralog": false, "identity": 0.9845559845559846, "score": 498.0, "evalue": 4.6e-180, "description": "Internal stop codon at 86418, 86445, 86517, 86544. Nonsense mutation around 86418, 86445, 86517, 86544.", "psc": { "uniref90_id": "UniRef90_C4XE16", "product": "DUF3137 domain-containing protein", "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE16", "UniRef:UniRef50_C4XE16" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16" ], "product": "DUF3137 domain-containing protein" } }, "id": "EFPKPENHBP_199", "locus": "GCA000186005_00159" }, { "type": "cds", "sequence": "contig_1", "start": 87116, "stop": 87286, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGATTTTTGTACCAAAGACTTTTGCGGAATTTAAAGATACAAATAAACAGTTTGTAAATGACAAAATACAATTAATTATAGACAATAAAAATAAAGAAATTAAGACAAGTTTTTTATATTTAATTACATCAATTGTTTTAATATTTCTAGGAATAATTTTTTGA", "aa": "MAKIFVPKTFAEFKDTNKQFVNDKIQLIIDNKNKEIKTSFLYLITSIVLIFLGIIF", "aa_hexdigest": "918a411f3120123dc14306e17b3dd998", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6553.787999999997, "isoelectric_point": 9.45688343048096 }, "id": "EFPKPENHBP_200", "locus": "GCA000186005_00160" }, { "type": "cds", "sequence": "contig_1", "start": 87410, "stop": 87655, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAAACAAAATTCGATTGTTACAAATATTGACAAAGGTCTAAATGAAGAAGATATTTACAAGCAAATTTTTAACCAAATTAACTATAAAATAGTTGAGTCGGAATATTTAGAAAATGTAAGTGAACAAGAAGAAAAGAATTTTTTATCAAGAGAATTAAAGAAAAAAAGTAATATAGAAGAAAGAAAAGAATTAACTAAAAAAATAAATGATATTTTAATTAATGAAATACCTTTTAACTGA", "aa": "MRKQNSIVTNIDKGLNEEDIYKQIFNQINYKIVESEYLENVSEQEEKNFLSRELKKKSNIEERKELTKKINDILINEIPFN", "aa_hexdigest": "a09586b7cf99781ca23b287d946dad0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9762.92729999999, "isoelectric_point": 5.2397253036499025 }, "id": "EFPKPENHBP_201", "locus": "GCA000186005_00161" }, { "type": "cds", "sequence": "contig_1", "start": 88037, "stop": 88126, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTTCGGTTAATCACTTTGAACAATATTTTGATAAAAACAAAGATTTTGGATTATTTTTAAAAAGAGATTTAATTAGAGGCTGA", "aa": "MEVSVNHFEQYFDKNKDFGLFLKRDLIRG", "aa_hexdigest": "a0119ad52b43e4144f5ae7d993f6585b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3547.004200000001, "isoelectric_point": 6.52206974029541 }, "id": "EFPKPENHBP_202", "locus": "GCA000186005_00162" }, { "type": "cds", "sequence": "contig_1", "start": 88289, "stop": 88408, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACATTTAATCAATTTTTATCTCGTTCATATGTTCCTTTGTCTAAACATTTAAATGAACAATTAAATAAAGAAGATTCAGAAAACAAAAAGCTTAGTAAATCTTTCTTATGA", "aa": "MKETFNQFLSRSYVPLSKHLNEQLNKEDSENKKLSKSFL", "aa_hexdigest": "fcb684531ba649301cf0f7cda11a0bc9", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.2364, "isoelectric_point": 9.224345970153806 }, "id": "EFPKPENHBP_203", "locus": "GCA000186005_00163" }, { "type": "cds", "sequence": "contig_1", "start": 88418, "stop": 88702, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTGTGTTTATTAATTATATTGACTTCAATTTCAATAGTTCTGATGTTGTCATTTTTTGAAAAGTCATATAAGATTGAAATTTTAAAATGGGCTACTGTTGCAATTTTGATTATAACAAATTTGTTTTGCGGAATAAGTTTTACATTTTCAAGAAAGGCAAAAGTTATTGAAAAAAGTCGAAATTTGGCAAAAATTAATCTTAATAATTCATTAAATTATTTATTTAAATTACTTAATTTTGAATACAAAAATGAAGAAAAAAATAACAATATTCCATGA", "aa": "MGLCLLIILTSISIVLMLSFFEKSYKIEILKWATVAILIITNLFCGISFTFSRKAKVIEKSRNLAKINLNNSLNYLFKLLNFEYKNEEKNNNIP", "aa_hexdigest": "7f2ddd0760bd29f8ea5d9ede8ebf7c38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10878.915399999985, "isoelectric_point": 9.554681968688964 }, "id": "EFPKPENHBP_204", "locus": "GCA000186005_00164" }, { "type": "cds", "sequence": "contig_1", "start": 88880, "stop": 89077, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAAATTCATATATTTCATGTGGAAGTAAAAATGAAATTTATGATTATTTAAAAAAGAAAAATAAAGTTAAATTTATTCATCAAAATGATCAAATTCATTTCAAAACTTCAAGTAAAGAAATATATGAACTAACTGACACAATTAAGACTTTTGAAGAATTTAAATTGAATGAAAAAGATTATGATTTTTAA", "aa": "MTENSYISCGSKNEIYDYLKKKNKVKFIHQNDQIHFKTSSKEIYELTDTIKTFEEFKLNEKDYDF", "aa_hexdigest": "688b8295a0d7b972ecd5bec4c87494d4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7909.776199999997, "isoelectric_point": 6.071563911437988 }, "id": "EFPKPENHBP_205", "locus": "GCA000186005_00165" }, { "type": "cds", "sequence": "contig_1", "start": 90100, "stop": 90306, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 90100, "stop": 90409, "observations": { "insertion": [ 90379 ], "mutation": [ 90304 ], "stop": [ 90304 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9901960784313726, "score": 192.0, "evalue": 5.56e-65, "description": "Internal stop codon at 90304. Frameshift due to insertion around 90379. Nonsense mutation around 90304.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "EFPKPENHBP_206", "locus": "GCA000186005_00166" }, { "type": "cds", "sequence": "contig_1", "start": 90370, "stop": 90594, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDELVSMNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "ee1a46cfc99fa70b8a20f44b791b3aad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8704.383199999993, "isoelectric_point": 10.111495399475096 }, "id": "EFPKPENHBP_207", "locus": "GCA000186005_00167" }, { "type": "cds", "sequence": "contig_1", "start": 90706, "stop": 90978, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTDNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "a6cf9241f23083862b43c93386ae60e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10698.441299999997, "isoelectric_point": 9.751826286315914 }, "id": "EFPKPENHBP_208", "locus": "GCA000186005_00168" }, { "type": "cds", "sequence": "contig_1", "start": 91817, "stop": 92317, "strand": "+", "frame": 2, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETELKRALTNDATSKLIKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYEKFIQYCAKRLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "db6b55899c8f181b6b7105e638679bda", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 91817, "stop": 92620, "observations": { "mutation": [ 92315, 92438, 92564 ], "stop": [ 92315, 92438, 92564 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9520000000000001, "score": 316.0, "evalue": 3.11e-107, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9550561797752809, "score": 510.0, "evalue": 3.67e-186, "description": "Internal stop codon at 92315, 92438, 92564. Nonsense mutation around 92315, 92438, 92564.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "EFPKPENHBP_209", "locus": "GCA000186005_00169" }, { "type": "cds", "sequence": "contig_1", "start": 93198, "stop": 93362, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATTTAAGTGAAAAATTAAAAAATTGTAGTGAGCAAGAATTAAAAGATTTCAATTATTTTATAGATGGTATGTTAGAAATGCCATTAATAAATGCTATTGATGAAGTAATTGATTGTCTTGAAAAACCTGATTATTGTGAACATTATGATACTCATTGA", "aa": "MSNLSEKLKNCSEQELKDFNYFIDGMLEMPLINAIDEVIDCLEKPDYCEHYDTH", "aa_hexdigest": "cf8540ac23419744ba1c09e0b3a718ca", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6386.1314, "isoelectric_point": 4.215486717224121 }, "id": "EFPKPENHBP_210", "locus": "GCA000186005_00170" }, { "type": "cds", "sequence": "contig_1", "start": 94082, "stop": 94552, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526654.1", "SO:0001217", "UniParc:UPI0001F59BE2", "UniRef:UniRef100_A0AB32XBB0", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAATATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAAAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESISKLIDSKTIINFDIAKLQNINKYFGFGISDINLLKINQVLFDYELLKTLRLENNLLQLNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEKKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "e28f1eb41085965ad430512c369d58be", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BE2", "ncbi_nrp_id": "WP_013526654.1", "uniref100_id": "UniRef100_A0AB32XBB0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE2", "RefSeq:WP_013526654.1", "UniRef:UniRef100_A0AB32XBB0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBB0", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18893.765299999992, "isoelectric_point": 9.172642326354982 }, "id": "EFPKPENHBP_211", "locus": "GCA000186005_00171" }, { "type": "cds", "sequence": "contig_1", "start": 94566, "stop": 94730, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESDNVGYYVTFPDNKRIKLNQELKMCLYEIKNDFT", "aa_hexdigest": "c8dc609ca0a49ee72ca57b660e6dd3fc", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6497.5178, "isoelectric_point": 9.09843921661377 }, "id": "EFPKPENHBP_212", "locus": "GCA000186005_00172" }, { "type": "cds", "sequence": "contig_1", "start": 94682, "stop": 94873, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAATCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAAGTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAACACATTTTTAATTCTTGA", "aa": "MLPSLIKFIALSNPTFKTLNATLGPMPFKLSKSANNFLSSNSLKPYKDQVKSFLISYKHIFNS", "aa_hexdigest": "2f86f56e58c2d4a6a30a4f5d75ffa7b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.271900000002, "isoelectric_point": 10.176221656799317 }, "id": "EFPKPENHBP_213", "locus": "GCA000186005_00173" }, { "type": "cds", "sequence": "contig_1", "start": 94985, "stop": 95167, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526656.1", "SO:0001217", "UniParc:UPI0001A8C12C", "UniRef:UniRef100_C4XEG5", "UniRef:UniRef50_C4XEG5", "UniRef:UniRef90_C4XEG5" ], "nt": "ATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAA", "aa": "MFENYVTTYKSSKEINETLKMLGFKDSLIDDVVRKINLNQDIEFSLEEAIKLMSDKPKTL", "aa_hexdigest": "c6bdaa9e4aa39c8bf37f7321449d566e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12C", "ncbi_nrp_id": "WP_013526656.1", "uniref100_id": "UniRef100_C4XEG5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12C", "RefSeq:WP_013526656.1", "UniRef:UniRef100_C4XEG5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG5", "uniref90_id": "UniRef90_C4XEG5" }, "psc": { "uniref90_id": "UniRef90_C4XEG5", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEG5" }, "pscc": { "uniref50_id": "UniRef50_C4XEG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG5" ], "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein" }, "genes": [ "ruvA" ], "id": "EFPKPENHBP_214", "locus": "GCA000186005_00174" }, { "type": "cds", "sequence": "contig_1", "start": 95186, "stop": 95626, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLQPITSNNIVDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRFKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "f2a1161e604ea609c72117339484b6f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 0.993, "score": 279.0, "evalue": 5.88e-95, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "EFPKPENHBP_215", "locus": "GCA000186005_00175" }, { "type": "cds", "sequence": "contig_1", "start": 95877, "stop": 96572, "strand": "+", "frame": 3, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGA", "aa": "MRKSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISMSTNYVKKYSEINKLTKPNVDIFDNLIKRLKIK", "aa_hexdigest": "9122dc5204b70e2613022a967e5f69c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "query_cov": 0.9653679653679653, "subject_cov": 1.0, "identity": 0.991, "score": 439.0, "evalue": 1.61e-155, "valid": true, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" }, "genes": [], "id": "EFPKPENHBP_216", "locus": "GCA000186005_00176" }, { "type": "cds", "sequence": "contig_1", "start": 97093, "stop": 97224, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGA", "aa": "MNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDE", "aa_hexdigest": "1bc3e1363e697e2abdb23ef609e7e097", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.7792, "isoelectric_point": 6.142328453063963 }, "id": "EFPKPENHBP_217", "locus": "GCA000186005_00177" }, { "type": "cds", "sequence": "contig_1", "start": 97225, "stop": 97560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_C4XEG8" ], "nt": "TTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAA", "aa": "MFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA", "aa_hexdigest": "05d6c4774ad92cc4040ff46cab4c8f18", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 97063, "stop": 97560, "observations": { "mutation": [ 97222 ], "stop": [ 97222 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEG8", "query_cov": 1.0, "subject_cov": 0.6727272727272727, "identity": 0.991, "score": 216.0, "evalue": 2.87e-70, "gene_start": 1, "gene_end": 111, "reference_start": 55, "reference_end": 165, "reference_sequence": "MNNYKKQGENMNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDEWLFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA" }, "paralog": false, "identity": 0.9878787878787879, "score": 325.0, "evalue": 1.23e-115, "description": "Internal stop codon at 97222. Nonsense mutation around 97222.", "psc": { "uniref90_id": "UniRef90_C4XEG8", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEG8", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12876.668799999981, "isoelectric_point": 8.799241447448733 }, "id": "EFPKPENHBP_218", "locus": "GCA000186005_00178" }, { "type": "cds", "sequence": "contig_1", "start": 97580, "stop": 97939, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKPYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFRKNGDTSCLTVL", "aa_hexdigest": "960afa9397d38dc95b18e550185890cb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14329.662599999978, "isoelectric_point": 9.686004066467284 }, "id": "EFPKPENHBP_219", "locus": "GCA000186005_00179" }, { "type": "cds", "sequence": "contig_1", "start": 98213, "stop": 98431, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "EFPKPENHBP_220", "locus": "GCA000186005_00180" }, { "type": "cds", "sequence": "contig_1", "start": 98466, "stop": 98912, "strand": "+", "frame": 3, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "4164854a7130acf16c96c160bbf5ab07", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 98427, "stop": 99056, "observations": { "mutation": [ 98910 ], "stop": [ 98910 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 9.07e-94, "description": "Internal stop codon at 98910. Nonsense mutation around 98910.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "EFPKPENHBP_221", "locus": "GCA000186005_00181" }, { "type": "cds", "sequence": "contig_1", "start": 98955, "stop": 99056, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 4.829350255693511e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "EFPKPENHBP_222", "locus": "GCA000186005_00182" }, { "type": "cds", "sequence": "contig_1", "start": 99062, "stop": 99202, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSKENNTIPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "cc5458cac4667834d74f24e26816b731", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5374.040500000001, "isoelectric_point": 8.099630546569824 }, "id": "EFPKPENHBP_223", "locus": "GCA000186005_00183" }, { "type": "cds", "sequence": "contig_1", "start": 99550, "stop": 99798, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAAATAACTTTTTTATCACTAAAACCTTCTGCATTTTCCTTTTTTATAGTTGGGTTTTTAGATGGTTTTGGTGGATTAATAGGAAAAGCAAACCAATTACAACTAGTTGCTGTTAAAGCAGTTACAATTGGTAATGTTATTGTTGGTAATATTGTCTTTAATCATATCTTTTTAGTTTTCATATTGCTCCTTATTTGCTATATAAATATTATACTTTATATTTATATTAAAATAAATCTTTAA", "aa": "MAEITFLSLKPSAFSFFIVGFLDGFGGLIGKANQLQLVAVKAVTIGNVIVGNIVFNHIFLVFILLLICYINIILYIYIKINL", "aa_hexdigest": "f50b57062a5a2a7a8f94084e8acefc52", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9064.948599999994, "isoelectric_point": 8.93223972320557 }, "id": "EFPKPENHBP_224", "locus": "GCA000186005_00184" }, { "type": "cds", "sequence": "contig_1", "start": 100046, "stop": 100204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGA", "aa": "MKYFENKKFEVLDNKEEVEKNHKSPTYIYKNYIECVDALGYFMENYKDPDLK", "aa_hexdigest": "84e4dd2b6bf174ecff57cd3cc33d7ffb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6403.183200000001, "isoelectric_point": 5.264279747009277 }, "id": "EFPKPENHBP_225", "locus": "GCA000186005_00185" }, { "type": "cds", "sequence": "contig_1", "start": 100400, "stop": 100708, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGA", "aa": "MNSKQIQALDKIYLMMEEATRAFYRVKHFENLCKPYNTIGNQINLYVSMINLFKNLYNLKIDTNKTLKEQLQDLKPEVLRALEKSKNALNKLLPIIDSGFGS", "aa_hexdigest": "1cdeb99ea6952a6abf34c664964b4a43", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.784499999983, "isoelectric_point": 9.380423927307131 }, "id": "EFPKPENHBP_226", "locus": "GCA000186005_00186" }, { "type": "cds", "sequence": "contig_1", "start": 101155, "stop": 101532, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5", "UniRef:UniRef50_C4XEH2", "UniRef:UniRef90_C4XEH2" ], "nt": "TTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAA", "aa": "MIKSKKNTSTISDSAFFAFGKEKMKITSLYYANNNPGISVLKNTSTYVLDEFVKYSAIIFGILFAIAFVTSIITIAMNAAFTSKGNNTPAKLKKALNGSLISCGVLLALLILGPGLCGLISNSLK", "aa_hexdigest": "541bd4002d390de57c1f8e6291c84a4a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BE8", "uniref100_id": "UniRef100_A0AB32XAQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAQ5", "uniref90_id": "UniRef90_C4XEH2" }, "psc": { "uniref90_id": "UniRef90_C4XEH2", "uniref50_id": "UniRef50_C4XEH2" }, "pscc": { "uniref50_id": "UniRef50_C4XEH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13307.625799999976, "isoelectric_point": 9.769168281555174 }, "id": "EFPKPENHBP_227", "locus": "GCA000186005_00187" }, { "type": "cds", "sequence": "contig_1", "start": 101673, "stop": 101909, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGA", "aa": "MIFGKPLSNINSYEDLKVLPGAFIGIAIFSICLFFIILAISAFKLSYNKSKESFQSFKASLKAIIPNLVFIIVIPILI", "aa_hexdigest": "c4a50977911cf7020c43ced63351ec3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8654.436699999991, "isoelectric_point": 9.519095420837402 }, "id": "EFPKPENHBP_228", "locus": "GCA000186005_00188" }, { "type": "cds", "sequence": "contig_1", "start": 102063, "stop": 102266, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGA", "aa": "MKDELSLLNSNMGSLFLNSFIAIFALYIVGNITINISINVYQAFFLFIQAPLISVVSIEDGGKKLKV", "aa_hexdigest": "bcb457581ccf759ebf931c004962c8ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7397.688899999997, "isoelectric_point": 5.970674705505371 }, "id": "EFPKPENHBP_229", "locus": "GCA000186005_00189" }, { "type": "cds", "sequence": "contig_1", "start": 102288, "stop": 102749, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGA", "aa": "MLAIMILNLSISFMVLFFSGASKIIKPMSDATGIDSGICALIVYTGALITSLSLNKVMFGIFGVEYTSYLGKFTKKLAAKATKKISKRKSNSKNNSSSEPETTSQTDYHPTGNAKNAELIIIMQNNNQTKKPETTPQTIGAAKKAKKPETLND", "aa_hexdigest": "f013f8d8f6533028ea4dd7abb837224c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16557.087599999977, "isoelectric_point": 9.739512825012206 }, "id": "EFPKPENHBP_230", "locus": "GCA000186005_00190" }, { "type": "cds", "sequence": "contig_1", "start": 102780, "stop": 102917, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAA", "aa": "MDQLSKTLRGEKDLKKTSEISKELSELIENESSIKRIITSMERKK", "aa_hexdigest": "90459cbe5d27ff28c389422274ab21d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5252.027499999999, "isoelectric_point": 9.156267356872558 }, "id": "EFPKPENHBP_231", "locus": "GCA000186005_00191" }, { "type": "cds", "sequence": "contig_1", "start": 103076, "stop": 103975, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAACCTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTCAAAATGATTTTGAAAGTCTTAGAAATATTGAAAACATAGATGTCTATTATTTAGTTATCTATGCTAATGAAATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGA", "aa": "MAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKPSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYSQNDFESLRNIENIDVYYLVIYANEMNKLKQYENEFIKSFETTKIPLNVVTGMELIDFANMIFKKNISKSDKQNYFVEELDTELTKELDMKMQGQKLNDKVIKKLQSDKEYKLISLLAPERVEFNSKYIKVDNEYLSFQTLVDFNSLQINEG", "aa_hexdigest": "fe22ce43ff026985cdcb565578cfb4d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35192.32100000002, "isoelectric_point": 9.08876895904541 }, "id": "EFPKPENHBP_232", "locus": "GCA000186005_00192" }, { "type": "cds", "sequence": "contig_1", "start": 104066, "stop": 104401, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGA", "aa": "MSLNEANAETKSNFKMQKISVEQKVLDELVDFINVQKMNLFDSNIIFVNQSKTLEGIKDLETKNDINAKKAFANVYKMHYLQKQALQNSIFIKDDFLNNGIEMSSRNIVYG", "aa_hexdigest": "d468523640bb645f71595be49c8c4145", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12798.509799999985, "isoelectric_point": 6.572315406799317 }, "id": "EFPKPENHBP_233", "locus": "GCA000186005_00193" }, { "type": "cds", "sequence": "contig_1", "start": 104537, "stop": 104668, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGA", "aa": "MPGSGKSATTSKIALHQFVVGNEVIIIDPQREYKDLCKKLDGN", "aa_hexdigest": "fc61c848a8aa12fc080e164cc7532130", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.368800000001, "isoelectric_point": 7.907514762878418 }, "id": "EFPKPENHBP_234", "locus": "GCA000186005_00194" }, { "type": "cds", "sequence": "contig_1", "start": 104675, "stop": 105508, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGA", "aa": "MGKGLETKINPLQINYEIEKDNIKVSDELLISNHTKMVKEFFSLLYPDLDARDLRVANFLLNEFYLSNKIKNIQKLKNTDFPTMKDYIKFLKSYKFKDKKAELYYKDSLNKLSIIFDQDFNNNGIFSNLYNGHTNINFNKTLTVIDTYNLINNIASNEGQASLYLILSYVRNRISNNFFENKTRKICLIVDEAHRFINANNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSDEVMNLTKGMVENIQYTLIFRQNQNGVNAISQMYKSKNELTN", "aa_hexdigest": "280b7bca572fc66e13b2387afc433124", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 49, "aa_cov": 0.16967509025270758, "hmm_cov": 1.0, "evalue": 4.151631598292585e-06, "score": 22.68251609802246, "start": 185, "stop": 231 } ], "seq_stats": { "molecular_weight": 32361.543500000033, "isoelectric_point": 8.832313728332519 }, "id": "EFPKPENHBP_235", "locus": "GCA000186005_00195" }, { "type": "cds", "sequence": "contig_1", "start": 105548, "stop": 105649, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAA", "aa": "MFSIDNKTRFQISIYYNQFEKDMIYKSGDLSNI", "aa_hexdigest": "035e85ec4bfd28e346bfa357462f8ecf", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.5033000000003, "isoelectric_point": 5.9190080642700185 }, "id": "EFPKPENHBP_236", "locus": "GCA000186005_00196" }, { "type": "cds", "sequence": "contig_1", "start": 105691, "stop": 105801, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGA", "aa": "MKKLKNFLLGISSTIPASGVFLISSTNSKNEDSFNN", "aa_hexdigest": "a0e4b77d48cb83e2dc872eacbda44321", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3903.415100000001, "isoelectric_point": 9.524639701843263 }, "id": "EFPKPENHBP_237", "locus": "GCA000186005_00197" }, { "type": "cds", "sequence": "contig_1", "start": 107356, "stop": 107472, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGA", "aa": "MEISNLAPGNEIKLTKNGFYLIYDISLDKTNINKFKIV", "aa_hexdigest": "46b9446de2d42f5c527e5fc58df131d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4359.047700000001, "isoelectric_point": 8.06391506195068 }, "id": "EFPKPENHBP_238", "locus": "GCA000186005_00198" }, { "type": "cds", "sequence": "contig_1", "start": 107508, "stop": 107744, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTGTCAAGATCTTTTTTTGTTGCGTTTAAATCAATAATTTTTTTCTTGGCTAAGTATTTTTCAAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTTTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAA", "aa": "MLISGLISKFSSKDFSIKKLLYSLKISSYFNLSRSFFVAFKSIIFFLAKYFSKSFETLRFLFLQNFLINDGEFASSVK", "aa_hexdigest": "ef675762ed1fa72f7ae50d5f3eee181e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9093.62449999999, "isoelectric_point": 9.997321891784669 }, "id": "EFPKPENHBP_239", "locus": "GCA000186005_00199" }, { "type": "cds", "sequence": "contig_1", "start": 107986, "stop": 108492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGA", "aa": "MKPIFIKFNNINIEEHIPFNVFVNELINNFNNLIESAKDINNEDIDLNIIELKILKESDENQIIVKAISKDANYYIANNDTYTYNVNLLKSDYSYLLNFNPGTLNVFGLKDKEEIKKYIKELLKNYLIQYNEKIDQSLYKKITIDDINISFNDEELNKIINPKDDDIY", "aa_hexdigest": "25aa009585af386a0ed42f8f656a45a5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19855.2482, "isoelectric_point": 4.60994930267334 }, "id": "EFPKPENHBP_240", "locus": "GCA000186005_00200" }, { "type": "cds", "sequence": "contig_1", "start": 108643, "stop": 109080, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGA", "aa": "MENKKDPVKMAQIKNETIRQVREKIIEEFKKRAPKLEIDKLYTIDFTDSFFNNLDLAIGTTLTLKGKHFMLKNPYTVNVINTHSSANKKLNLKDLNFEVITLSEKNLNDFKKALNEKIIDILKKENYPAKEIIKLIEFKLLENQK", "aa_hexdigest": "8d500fe1206dd8cb856ae29ceacad26a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17024.737599999993, "isoelectric_point": 9.435415458679199 }, "id": "EFPKPENHBP_241", "locus": "GCA000186005_00201" }, { "type": "cds", "sequence": "contig_1", "start": 109120, "stop": 109257, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAGAAAAAATTACTTTCATGA", "aa": "MMIQLKEESNLISGDKEFKLINIFTEEMLKEALEIKSKNKKKLLS", "aa_hexdigest": "4b6526b274932fbecbd98c07284bc658", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5284.2575, "isoelectric_point": 8.009826087951662 }, "id": "EFPKPENHBP_242", "locus": "GCA000186005_00202" }, { "type": "cds", "sequence": "contig_1", "start": 109420, "stop": 110166, "strand": "+", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "c3fcc899803f7ec5ead3347237f6e376", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 109345, "stop": 110469, "observations": { "mutation": [ 110164, 110308 ], "stop": [ 110164, 110308 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 1.0, "score": 484.0, "evalue": 3.89e-170, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9919786096256684, "score": 742.0, "evalue": 5.33e-274, "description": "Internal stop codon at 110164, 110308. Nonsense mutation around 110164, 110308.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "EFPKPENHBP_243", "locus": "GCA000186005_00203" }, { "type": "cds", "sequence": "contig_1", "start": 110362, "stop": 110640, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTYH", "aa_hexdigest": "5dd97fea0cd43f7cf7d9092425b8453f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8141592920353983, "identity": 0.9890000000000001, "score": 177.0, "evalue": 2.98e-56, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10826.261399999988, "isoelectric_point": 7.949612617492677 }, "id": "EFPKPENHBP_244", "locus": "GCA000186005_00204" }, { "type": "cds", "sequence": "contig_1", "start": 110981, "stop": 111331, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "6a907dccef0cbbba0bbf260e0f56003e", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13576.067399999976, "isoelectric_point": 9.912288093566893 }, "id": "EFPKPENHBP_245", "locus": "GCA000186005_00205" }, { "type": "cds", "sequence": "contig_1", "start": 111466, "stop": 112014, "strand": "-", "frame": 1, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "RefSeq:WP_013526667.1", "SO:0001217", "UniParc:UPI0001F59D2D", "UniRef:UniRef100_C4XE55", "UniRef:UniRef50_A0A3P8MDG1", "UniRef:UniRef90_C4XE55" ], "nt": "ATGTTAAGAATTATCAGTGGAAAATATCGTCATTTAAATATAGAACAACCTAAAACTTTGGAAACAAGACCAACTATGGATCGTGTTCGTGAAGCTATTTTTTCATCAATAAGATTTAACTTAGAAGGTGCTTGCATTTTAGATCTTTTTGCAGGTAGTGGTGCTTTTAGTTTTGAAGCATTAAGCAACTATGCGGTAAAAGCAATAGCTGTTGATAATAATAATGAAGCAATTAAAGCAATAAGAAATAATGCTTTAAAACTAAAAGCTGAAAATATCGATATTGTAAAAGATGATGTTTTGAATTATTTACAAAAGAATTCGGGACGTAAATTTGATTTTATTTTTATTGATGCTCCATACAAGGAATATCAATTGATTAATAAATCTCTTGAATTAATAGTTGATAATAAATTTTTAACTAAAAATGGTTTAATTATTTTAGAAACTGACAATTTCTTAATGATTGATATTCCAAAAAAGCTTACAATACAAAAACAAAAACAATATGGAAAAGTAAATATTTTGTTTATCTCAAATAATAATTAA", "aa": "MLRIISGKYRHLNIEQPKTLETRPTMDRVREAIFSSIRFNLEGACILDLFAGSGAFSFEALSNYAVKAIAVDNNNEAIKAIRNNALKLKAENIDIVKDDVLNYLQKNSGRKFDFIFIDAPYKEYQLINKSLELIVDNKFLTKNGLIILETDNFLMIDIPKKLTIQKQKQYGKVNILFISNNN", "aa_hexdigest": "39e8e1dbd7c392c265564285e26a746c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F59D2D", "ncbi_nrp_id": "WP_013526667.1", "uniref100_id": "UniRef100_C4XE55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D2D", "RefSeq:WP_013526667.1", "UniRef:UniRef100_C4XE55" ] }, "ips": { "uniref100_id": "UniRef100_C4XE55", "uniref90_id": "UniRef90_C4XE55" }, "psc": { "uniref90_id": "UniRef90_C4XE55", "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A3P8MDG1", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDG1" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "EFPKPENHBP_246", "locus": "GCA000186005_00206" }, { "type": "cds", "sequence": "contig_1", "start": 112085, "stop": 112654, "strand": "+", "frame": 2, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "RefSeq:WP_013526668.1", "SO:0001217", "UniParc:UPI0001F59D2E", "UniRef:UniRef100_C4XE56", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_C4XE56" ], "nt": "ATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTATATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAACCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAA", "aa": "MKYEIKLVELPEKILRKKSKEVKIPLSKEDEELAKKMIYHIDESQKEDSKLGFRPGVGVAAIQYGIPKRMFYININNETINGAKIEDFRDVLINPKVLATSEYEVSLPGEGCLSVGDNIKNQEGYVYRKKRIVLEAYSYFEKKVKHYDLVDYAAIVAQHEFDHLEGKLFIDRINKKDPFVIKDDSEILE", "aa_hexdigest": "be95a1707f560b5646e6a1050d46db22", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59D2E", "ncbi_nrp_id": "WP_013526668.1", "uniref100_id": "UniRef100_C4XE56", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D2E", "RefSeq:WP_013526668.1", "UniRef:UniRef100_C4XE56" ] }, "ips": { "uniref100_id": "UniRef100_C4XE56", "uniref90_id": "UniRef90_C4XE56" }, "psc": { "uniref90_id": "UniRef90_C4XE56", "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "EFPKPENHBP_247", "locus": "GCA000186005_00207" }, { "type": "cds", "sequence": "contig_1", "start": 112744, "stop": 113454, "strand": "+", "frame": 1, "gene": "cvpA", "product": "CvpA family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57", "UniRef:UniRef90_C4XE57" ], "nt": "TTGATAGTCCTTTTGGTCGCAATTGCTATTGGGGCTAAAAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTATGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGA", "aa": "MIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNYAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLAS", "aa_hexdigest": "bb72a2cc7bcdc5efd433bcd9371bc673", "start_type": "TTG", "rbs_motif": null, "genes": [ "cvpA" ], "truncated": "both", "pseudogene": { "start": 112651, "stop": 113757, "observations": { "mutation": [ 112690, 112714, 113452, 113494, 113623 ], "stop": [ 112690, 112714, 113452, 113494, 113623 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE57", "query_cov": 1.0, "subject_cov": 0.48459958932238195, "identity": 0.992, "score": 437.0, "evalue": 1.15e-150, "gene_start": 1, "gene_end": 236, "reference_start": 32, "reference_end": 267, "reference_sequence": "MINEVFMRTYEDNWKALATNGWCVLISISVFLIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNSAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLASWSDEEIERLPKVIDWLNYCFNDEASFDLLNNSYNYPTGETIKYYFKGVYSNDPSKATWNTKVLKYGNVYYYKKMNFGGPDSWSPATTTYAESLENKFDFKTMFSWNIFKWSQTLRTLSKIDLDSKFEVSDSKVEDFTKIKKIKNKDIQFKLNVENEVVRNKIKSIFYKLISLENEISNTKIDESKVLGFSTDSANKNQINDNTIKRFQLYFDNLLDTIMG" }, "paralog": false, "identity": 0.9809782608695652, "score": 703.0, "evalue": 4.6e-258, "description": "Internal stop codon at 112690, 112714, 113452, 113494, 113623. Nonsense mutation around 112690, 112714, 113452, 113494, 113623.", "psc": { "uniref90_id": "UniRef90_C4XE57", "product": "CvpA family protein", "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE57", "UniRef:UniRef50_C4XE57" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57" ], "product": "CvpA family protein" } }, "id": "EFPKPENHBP_248", "locus": "GCA000186005_00208" }, { "type": "cds", "sequence": "contig_1", "start": 113956, "stop": 114114, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTCAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAA", "aa": "MISLENEISNTKIDESKVLGFSTDSANKNQINDNTIKRFQLYFDNLLDTIMG", "aa_hexdigest": "47c6fdda95958ade5fbdcc9d29a5f90a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5940.5796, "isoelectric_point": 4.4576208114624025 }, "id": "EFPKPENHBP_249", "locus": "GCA000186005_00209" }, { "type": "cds", "sequence": "contig_1", "start": 114265, "stop": 114381, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2", "UniRef:UniRef90_UPI0020BDD874" ], "nt": "ATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGA", "aa": "MQKYDESSIQQLKGLEAVRKRPGMYIGSTDVNGLHHLI", "aa_hexdigest": "b449758f9a54df4fdf5a724069df0dba", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 114274, "stop": 114405, "observations": { "mutation": [ 114379 ], "stop": [ 114379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0020BDD874", "query_cov": 0.9210526315789473, "subject_cov": 0.660377358490566, "identity": 0.8, "score": 60.8, "evalue": 1.33e-11, "gene_start": 4, "gene_end": 38, "reference_start": 11, "reference_end": 45, "reference_sequence": "MEQKQMPENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSI" }, "paralog": false, "identity": 0.813953488372093, "score": 72.0, "evalue": 5.04e-19, "description": "Internal stop codon at 114379. Nonsense mutation around 114379.", "psc": { "uniref90_id": "UniRef90_UPI0020BDD874", "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0020BDD874", "UniRef:UniRef50_A0A829GPU2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "EFPKPENHBP_250", "locus": "GCA000186005_00210" }, { "type": "cds", "sequence": "contig_1", "start": 114472, "stop": 114768, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "nt": "ATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATTGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGA", "aa": "MDNGRGIPVGKTPSGKSAVELVFTELHAGGKFNEGAYKTSGGLHGVGSSVVNALSTKLIVTVYRDKKIYETIFENGDNIVQRTLEVGNTNKHGTMVEF", "aa_hexdigest": "a1a737656f6194c0eeefed80e0d6070d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920EUL8", "query_cov": 0.8163265306122449, "subject_cov": 0.8333333333333334, "identity": 0.588, "score": 102.0, "evalue": 1.67e-26, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920EUL8" }, "pscc": { "uniref50_id": "UniRef50_A0A920EUL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "EFPKPENHBP_251", "locus": "GCA000186005_00211" }, { "type": "cds", "sequence": "contig_1", "start": 114904, "stop": 115335, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00204.31" ], "nt": "ATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGA", "aa": "MYEDGLHAFVDFINDSKNALGDIKTFKETKRDIEVECGFQYTDSYNETVLSFVNNVKTKDGGTHEIGLKSALTKTFNEFALEEKALKGKNTFDGDDIREGLTIILSVKIPEKLLEFVSQTKEKLGTPEAKGVVEEIVSKNFKI", "aa_hexdigest": "d3567811b9e0fe67cd93cb1d3264be85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00204.31", "name": "DNA_gyraseB", "length": 142, "aa_cov": 0.965034965034965, "hmm_cov": 0.9859154929577465, "evalue": 4.898635986848452e-46, "score": 152.67640686035156, "start": 5, "stop": 142 } ], "seq_stats": { "molecular_weight": 16044.96759999999, "isoelectric_point": 5.041698265075683 }, "id": "EFPKPENHBP_252", "locus": "GCA000186005_00212" }, { "type": "cds", "sequence": "contig_1", "start": 115501, "stop": 115908, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGCTGAAAAAGAATTATTCTTAGTCGAAGGTGACTCTGCTGGAGGTAGTGCAAAACTTGGACGTGATCGTAAATATCAAGCAATTTTACCTTTAAGAGGAAAAGTTATTAATACTGAAAAAGCTCGTCTTTTTGAGATTTTAAAAAATGAAGAAATTGGAACAATAATTAATACAATCGGCGCTGGTGTTGGTGACGAATTTGACCTTAAAAAAGCTCAATACGGAAAAGTTGTTATTATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGA", "aa": "MPAEKELFLVEGDSAGGSAKLGRDRKYQAILPLRGKVINTEKARLFEILKNEEIGTIINTIGAGVGDEFDLKKAQYGKVVIMTDADTDGAHIQILLLTFFFRHMRQLVENGMVYIALPPLFKLTSTRGKKEVLYA", "aa_hexdigest": "e22811784afa56e0e4e29a121b6eb988", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC415C3", "query_cov": 0.9629629629629629, "subject_cov": 0.8609271523178808, "identity": 0.5770000000000001, "score": 162.0, "evalue": 6.93e-49, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A353NP07" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15027.384699999982, "isoelectric_point": 8.829992866516115 }, "id": "EFPKPENHBP_253", "locus": "GCA000186005_00213" }, { "type": "cds", "sequence": "contig_1", "start": 116020, "stop": 116130, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGA", "aa": "MNPITRTLVQVKIEDAALAERRVSTLMGDNVEPRKE", "aa_hexdigest": "8cc26119281ffd9c9602e0f30e1db464", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4081.676299999999, "isoelectric_point": 6.16506404876709 }, "id": "EFPKPENHBP_254", "locus": "GCA000186005_00214" }, { "type": "cds", "sequence": "contig_1", "start": 116191, "stop": 116391, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGA", "aa": "MNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQ", "aa_hexdigest": "488e542d0a74fbc41414112f39c48729", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 24, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 2.6796495315950306e-12, "score": 42.56110763549805, "start": 40, "stop": 63 } ], "seq_stats": { "molecular_weight": 7887.083800000003, "isoelectric_point": 9.62727336883545 }, "id": "EFPKPENHBP_255", "locus": "GCA000186005_00215" }, { "type": "cds", "sequence": "contig_1", "start": 116506, "stop": 117936, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2", "UniRef:UniRef90_C4XE59" ], "nt": "ATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAACAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGA", "aa": "MGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDNKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESK", "aa_hexdigest": "9c5f5778615b5d8b3c7ede33755da640", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 116206, "stop": 118239, "observations": { "mutation": [ 116389, 116500, 117934 ], "stop": [ 116389, 116500, 117934 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE59", "query_cov": 1.0, "subject_cov": 0.5554259043173863, "identity": 0.998, "score": 921.0, "evalue": 0.0, "gene_start": 1, "gene_end": 476, "reference_start": 107, "reference_end": 582, "reference_sequence": "MMNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQWNKPFKKSARVVGDVIGKYHPHGDSSIYEAMVRMAQDWKMGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDKKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESKWKDFGVHLSDFVELVSGEELVGAINVTDFNIQNYVCLFTKEAQGKRVVLKEFEVSRPNKTFTAMKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK" }, "paralog": false, "identity": 0.9940915805022157, "score": 1314.0, "evalue": 0.0, "description": "Internal stop codon at 116389, 116500, 117934. Nonsense mutation around 116389, 116500, 117934.", "psc": { "uniref90_id": "UniRef90_C4XE59", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE59", "UniRef:UniRef50_I5D5N2" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "EFPKPENHBP_256", "locus": "GCA000186005_00216" }, { "type": "cds", "sequence": "contig_1", "start": 118123, "stop": 118761, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAA", "aa": "MKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK", "aa_hexdigest": "db991e1d924fe66d1fc9ba1eb9d19c86", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23818.17920000002, "isoelectric_point": 8.704215049743652 }, "id": "EFPKPENHBP_257", "locus": "GCA000186005_00217" }, { "type": "cds", "sequence": "contig_1", "start": 118860, "stop": 119135, "strand": "+", "frame": 3, "gene": null, "product": "Phosphoesterase", "db_xrefs": [ "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_C4XE60", "UniRef:UniRef90_C4XE60" ], "nt": "ATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGA", "aa": "MEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDY", "aa_hexdigest": "afec43428a0c5d56c07ac79c75d19c96", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 118818, "stop": 119384, "observations": { "mutation": [ 118827, 119133, 119286 ], "stop": [ 118827, 119133, 119286 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE60", "query_cov": 1.0, "subject_cov": 0.4789473684210526, "identity": 1.0, "score": 194.0, "evalue": 6.67e-62, "gene_start": 1, "gene_end": 91, "reference_start": 17, "reference_end": 107, "reference_sequence": "MWIIFWFYFIILKEKIMEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDYWSLRTPNVLFDETKNIDCDFLICGHSHFPIYYKDVKTHKILLNPGSPSLPRWNSKPSYAIITIKNEKKYENVEVIFKEVNEKS" }, "paralog": false, "identity": 0.9840425531914894, "score": 384.0, "evalue": 7.26e-139, "description": "Internal stop codon at 118827, 119133, 119286. Nonsense mutation around 118827, 119133, 119286.", "psc": { "uniref90_id": "UniRef90_C4XE60", "product": "Phosphoesterase", "uniref50_id": "UniRef50_C4XE60", "kegg_orthology_id": "K07095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE60", "UniRef:UniRef50_C4XE60", "KEGG:K07095" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE60" ], "product": "Phosphoesterase" } }, "id": "EFPKPENHBP_258", "locus": "GCA000186005_00218" }, { "type": "cds", "sequence": "contig_1", "start": 119456, "stop": 119620, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGATTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGA", "aa": "MINKKNKPQYLFAIDLDGTLLASSATGEVHNTTAEAIARAKKEGHIVCALTGRP", "aa_hexdigest": "6646c448f929cf0f731a0bb4e3ebf6c7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7962962962962963, "hmm_cov": 0.9534883720930233, "evalue": 4.107411922435321e-10, "score": 36.10429382324219, "start": 12, "stop": 54 } ], "seq_stats": { "molecular_weight": 5765.5797999999995, "isoelectric_point": 8.979430580139162 }, "id": "EFPKPENHBP_259", "locus": "GCA000186005_00219" }, { "type": "cds", "sequence": "contig_1", "start": 120170, "stop": 120388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTAGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAA", "aa": "MFKVANISVAVGNAPDAVKKYATIKLKKTNRQGAVSEYINKFLDNPEKEIEKSNKVMAKLIKRRDEEADLED", "aa_hexdigest": "02c26970f6095ab68012cd4097b4307d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 39, "aa_cov": 0.5416666666666666, "hmm_cov": 0.9743589743589743, "evalue": 1.3370661111574368e-07, "score": 27.871370315551758, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 8153.306099999999, "isoelectric_point": 9.222154045104979 }, "id": "EFPKPENHBP_260", "locus": "GCA000186005_00220" }, { "type": "cds", "sequence": "contig_1", "start": 120391, "stop": 120822, "strand": "+", "frame": 1, "gene": null, "product": "Ribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7", "UniRef:UniRef90_C4XE62" ], "nt": "ATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGA", "aa": "MKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNN", "aa_hexdigest": "90c5062305b19d17c4a57a36634ae7b4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 120388, "stop": 121125, "observations": { "mutation": [ 120820 ], "stop": [ 120820 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE62", "query_cov": 1.0, "subject_cov": 0.572, "identity": 1.0, "score": 285.0, "evalue": 7.11e-96, "gene_start": 1, "gene_end": 143, "reference_start": 2, "reference_end": 144, "reference_sequence": "MMKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNNWADFNQIQGDILLAYKAESISLEVACASIIARDYFLYFMQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKNFKMDLEDQNTTTINLFATDFENKK" }, "paralog": false, "identity": 0.9918367346938776, "score": 491.0, "evalue": 2.18e-179, "description": "Internal stop codon at 120820. Nonsense mutation around 120820.", "psc": { "uniref90_id": "UniRef90_C4XE62", "product": "Ribonuclease", "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE62", "UniRef:UniRef50_A0A6M4JBT7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7" ], "product": "Ribonuclease" } }, "id": "EFPKPENHBP_261", "locus": "GCA000186005_00221" }, { "type": "cds", "sequence": "contig_1", "start": 120934, "stop": 121140, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAATTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAA", "aa": "MQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKNFKMDLEDQNTTTINLFATDFENKK", "aa_hexdigest": "0bd711aaf46cf49880db4efe71bc2519", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8046.993199999998, "isoelectric_point": 8.599067115783694 }, "id": "EFPKPENHBP_262", "locus": "GCA000186005_00222" }, { "type": "cds", "sequence": "contig_1", "start": 121410, "stop": 122021, "strand": "+", "frame": 3, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "nt": "ATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAACTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAAAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGACGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGA", "aa": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEKAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDD", "aa_hexdigest": "1c4064bcd07e3e7fa96728bdaa5d15b0", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A7Y0YRM2", "query_cov": 0.9753694581280788, "subject_cov": 0.8181818181818182, "identity": 0.7020000000000001, "score": 273.0, "evalue": 4.43e-90, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_W7C0L0" }, "pscc": { "uniref50_id": "UniRef50_W7C0L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "EFPKPENHBP_263", "locus": "GCA000186005_00223" }, { "type": "cds", "sequence": "contig_1", "start": 122094, "stop": 123167, "strand": "+", "frame": 3, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "GO:0005524", "GO:0006457", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_C4XE63" ], "nt": "ATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGCGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAG", "aa": "MAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE", "aa_hexdigest": "f932c8163746fec5f053c5d8492b0d77", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PNF8", "query_cov": 0.8991596638655462, "subject_cov": 0.9250720461095101, "identity": 0.579, "score": 346.0, "evalue": 4.94e-115, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_A0A8S2D270" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 121794, "stop": 123167, "observations": { "mutation": [ 122019, 122040 ], "stop": [ 122019, 122040 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE63", "query_cov": 1.0, "subject_cov": 0.6102564102564103, "identity": 1.0, "score": 645.0, "evalue": 2.81e-229, "gene_start": 1, "gene_end": 357, "reference_start": 229, "reference_end": 585, "reference_sequence": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEKAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDDWDHEIVDWLVAKIKNDHKIDIRENKMAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE" }, "paralog": false, "identity": 0.9956236323851203, "score": 861.0, "evalue": 1.13e-316, "description": "Internal stop codon at 122019, 122040. Nonsense mutation around 122019, 122040.", "psc": { "uniref90_id": "UniRef90_C4XE63", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE63", "UniRef:UniRef50_Q49539", "GO:0005524", "GO:0006457", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "EFPKPENHBP_264", "locus": "GCA000186005_00224" }, { "type": "cds", "sequence": "contig_1", "start": 123356, "stop": 124570, "strand": "+", "frame": 2, "gene": null, "product": "AAA+ ATPase domain-containing protein", "db_xrefs": [ "KEGG:K07478", "RefSeq:WP_013354366.1", "SO:0001217", "UniParc:UPI0001E32F5C", "UniRef:UniRef100_C4XE64", "UniRef:UniRef50_A0A449A4D0", "UniRef:UniRef90_C4XE64" ], "nt": "ATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATCAGCATTTCATAAATCATTAAGAGGAAGTGATGTTGATGCAGCACTTTATTATGGAGCTTTAATCTTAAAAACAGGCGACTACATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAA", "aa": "MKNLANEIRPRTLDDIVGQKHVVSLLKEIVKNKASTSFIFFGESGTGKSSAAVALANDLKLKYGYFNATINNKDELVNTIQTNDIIIIDEIHRLNKDKQDILLSYLEYDKIIVYATTTENPYFKVNPALRSRMQILQFNKLSEEEILFGLKKIIKTYYPKFKIKDDILKVLIKNSTGDYRSCINNLQILTLLNKDKEVTEESIKTLIPNVNFYSDKESNAHYNNLSAFHKSLRGSDVDAALYYGALILKTGDYMGLFRRLTAATYEDVGLADPTLPMRVEAAFNAVERLGFPEAYLPLYYIIISVAIAPKSNTVYKAIQKVEPFIEQGNIYEIPKHLRDAHYKSASKLGDGLDYKYPHDFPNHWVKQEYLPKRIASKKFFEPDNNDSPSLLKYYNFIKNIKGDK", "aa_hexdigest": "8c9719697d297e25499b5530e32b7687", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F5C", "ncbi_nrp_id": "WP_013354366.1", "uniref100_id": "UniRef100_C4XE64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F5C", "RefSeq:WP_013354366.1", "UniRef:UniRef100_C4XE64" ] }, "ips": { "uniref100_id": "UniRef100_C4XE64", "uniref90_id": "UniRef90_C4XE64" }, "psc": { "uniref90_id": "UniRef90_C4XE64", "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_A0A449A4D0", "kegg_orthology_id": "K07478" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4D0" ], "product": "ATPase" }, "genes": [], "id": "EFPKPENHBP_265", "locus": "GCA000186005_00225" }, { "type": "cds", "sequence": "contig_1", "start": 124998, "stop": 125219, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGCAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCAACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGA", "aa": "MHDSLYLNPTTLLRTHNTGISALELERNANKEMNNFAIGKVYRNDEDDATHSHQFTQLDFVSVGKSVSFNNLI", "aa_hexdigest": "803e37801c05792fafefc81ae2c211d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 79, "aa_cov": 0.9863013698630136, "hmm_cov": 1.0, "evalue": 8.828465023540772e-21, "score": 70.78111267089844, "start": 1, "stop": 72 } ], "seq_stats": { "molecular_weight": 8295.0804, "isoelectric_point": 5.667268180847168 }, "id": "EFPKPENHBP_266", "locus": "GCA000186005_00226" }, { "type": "cds", "sequence": "contig_1", "start": 125361, "stop": 125534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATACAACATCAAGGATGTTAGAGAATTCTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAG", "aa": "MLHPNVMELAGYDSKEFNGFAAGIGIERLTMIKYNIKDVREFYKNDLRTLNQFNYEE", "aa_hexdigest": "e6961cf0370b84c54345c6dea50dd53e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 53, "aa_cov": 0.9298245614035088, "hmm_cov": 0.9811320754716981, "evalue": 1.75596770566361e-19, "score": 66.52803802490234, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 6748.5875, "isoelectric_point": 5.2735445022583 }, "id": "EFPKPENHBP_267", "locus": "GCA000186005_00227" }, { "type": "cds", "sequence": "contig_1", "start": 125524, "stop": 125850, "strand": "+", "frame": 1, "gene": null, "product": "uracil-DNA glycosylase", "db_xrefs": [ "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_N9V498", "UniRef:UniRef90_C4XE67" ], "nt": "ATGAAGAATAGCTTCCAAACATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCTTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGA", "aa": "MKNSFQTFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDLYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKS", "aa_hexdigest": "bdd0a050bbded19856dbb92b63064677", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 125512, "stop": 126153, "observations": { "mutation": [ 125848, 125929, 125998 ], "stop": [ 125848, 125929, 125998 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE67", "query_cov": 1.0, "subject_cov": 0.47577092511013214, "identity": 0.991, "score": 211.0, "evalue": 1.8e-67, "gene_start": 1, "gene_end": 108, "reference_start": 5, "reference_end": 112, "reference_sequence": "MNLTMKNSFQTFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDPYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKSWAKQGVLLINTCLSVNENQPLSHKNFGWEIFVINLIKYILKKNKNVIFGIWGNDAKRILQIVQNDYKIDENKIIFTSHPSPLAYSKTTNSFKDSKFFIRVNSLLKNPINWDLRKD" }, "paralog": false, "identity": 0.9765258215962441, "score": 412.0, "evalue": 4.03e-149, "description": "Internal stop codon at 125848, 125929, 125998. Nonsense mutation around 125848, 125929, 125998.", "psc": { "uniref90_id": "UniRef90_C4XE67", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_N9V498", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE67", "EC:3.2.2.27", "UniRef:UniRef50_N9V498", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_N9V498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V498" ], "product": "Uracil-DNA glycosylase" } }, "id": "EFPKPENHBP_268", "locus": "GCA000186005_00228" }, { "type": "cds", "sequence": "contig_1", "start": 126022, "stop": 126177, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTGTTCAAAATGACTATAAAATAGATGAAAACAAAATTATTTTTACTTCTCACCCTTCCCCTCTTGCATATTCAAAAACAACTAATAGTTTTAAAGATTCAAAGTTTTTTATAAGAGTAAATTCATTATTAAAAAATCCAATTAATTGA", "aa": "MQIVQNDYKIDENKIIFTSHPSPLAYSKTTNSFKDSKFFIRVNSLLKNPIN", "aa_hexdigest": "e006ee88406ae666a4c667a6cba69e7e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5946.741299999999, "isoelectric_point": 9.52289905548096 }, "id": "EFPKPENHBP_269", "locus": "GCA000186005_00229" }, { "type": "cds", "sequence": "contig_1", "start": 126197, "stop": 126775, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATTAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAGAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGCGACTTTGTATTTCAAGATAAAGAAATGGCAGGCGTTGTTTCTCAAGGTATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGA", "aa": "MIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFK", "aa_hexdigest": "0ebbddf26e49b7727938c6bf77eb5f09", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 84, "aa_cov": 0.40625, "hmm_cov": 0.9047619047619048, "evalue": 4.856509411092615e-08, "score": 29.353538513183594, "start": 44, "stop": 121 } ], "seq_stats": { "molecular_weight": 21730.671700000006, "isoelectric_point": 6.372071647644043 }, "id": "EFPKPENHBP_270", "locus": "GCA000186005_00230" }, { "type": "cds", "sequence": "contig_1", "start": 127142, "stop": 128074, "strand": "+", "frame": 2, "gene": null, "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A3P8L797", "UniRef:UniRef90_C4XE68" ], "nt": "ATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGA", "aa": "MGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIG", "aa_hexdigest": "24897d433d68fab6222ca4e15ece78af", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 126842, "stop": 128377, "observations": { "mutation": [ 126944, 128072, 128114 ], "stop": [ 126944, 128072, 128114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE68", "query_cov": 1.0, "subject_cov": 0.4246575342465753, "identity": 1.0, "score": 612.0, "evalue": 4.72e-215, "gene_start": 1, "gene_end": 310, "reference_start": 318, "reference_end": 627, "reference_sequence": "MNMIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFKWFDYKKNYKTGIHKQIQKVDNGEANALSFLECRFKHKDTKIEDMLFLAKHNVEAKNVWAIDVTNLNLIYTGAPTHVYDRSEIGEELTCKKYSGKVTILGNKEIEVKNALAIYSKDKVVSLASVMGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIGWLGKVNPSYNSVDAWVCEVHKEVNKGYKLFENYDSTPFKSIDLTFELKPHQSIDDKIIEIKKVAFIFEIYQIDEFAKPESRNVTLRIIGSNEEIQKINNEFNN" }, "paralog": false, "identity": 0.9941291585127201, "score": 1004.0, "evalue": 0.0, "description": "Internal stop codon at 126944, 128072, 128114. Nonsense mutation around 126944, 128072, 128114.", "psc": { "uniref90_id": "UniRef90_C4XE68", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A3P8L797", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE68", "EC:6.1.1.20", "UniRef:UniRef50_A0A3P8L797", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L797", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L797" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "EFPKPENHBP_271", "locus": "GCA000186005_00231" }, { "type": "cds", "sequence": "contig_1", "start": 128392, "stop": 128931, "strand": "+", "frame": 1, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "EC:2.1.2.1", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P56089", "UniRef:UniRef90_C4XE69" ], "nt": "ATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCGGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAACCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGA", "aa": "MYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIID", "aa_hexdigest": "d9f4a5bd7785b51daa98e8967244b4ad", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002618815A", "query_cov": 0.9776536312849162, "subject_cov": 0.8293838862559242, "identity": 0.5770000000000001, "score": 197.0, "evalue": 3.74e-61, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A533YJM1", "kegg_orthology_id": "K00600" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 128380, "stop": 129234, "observations": { "mutation": [ 128929, 129139 ], "stop": [ 128929, 129139 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE69", "query_cov": 1.0, "subject_cov": 0.4221698113207547, "identity": 1.0, "score": 345.0, "evalue": 1.87e-116, "gene_start": 1, "gene_end": 179, "reference_start": 5, "reference_end": 183, "reference_sequence": "MGGIMYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIIDWAKFREIADACGAYLMADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDRWVFPGYQGGPLFHTIAGKAVAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTKWRELADIIDTILKNHETILKPESEKLFNSLKKKVRDFTKKFPIITVYR" }, "paralog": false, "identity": 0.9894366197183099, "score": 554.0, "evalue": 5.37e-201, "description": "Internal stop codon at 128929, 129139. Nonsense mutation around 128929, 129139.", "psc": { "uniref90_id": "UniRef90_C4XE69", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "kegg_orthology_id": "K00600", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE69", "EC:2.1.2.1", "UniRef:UniRef50_P56089", "KEGG:K00600" ] }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" } }, "id": "EFPKPENHBP_272", "locus": "GCA000186005_00232" }, { "type": "cds", "sequence": "contig_1", "start": 128974, "stop": 129141, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTATGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGA", "aa": "MADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDR", "aa_hexdigest": "345e31943a1700467e5aca3128ae9edf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 46, "aa_cov": 0.8363636363636363, "hmm_cov": 1.0, "evalue": 2.813820050770408e-22, "score": 75.16732025146484, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 5839.704599999999, "isoelectric_point": 8.247972297668458 }, "id": "EFPKPENHBP_273", "locus": "GCA000186005_00233" }, { "type": "cds", "sequence": "contig_1", "start": 129196, "stop": 129510, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGA", "aa": "MAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTK", "aa_hexdigest": "11dd0db7be7e1ddd133574db06e7805b", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 102, "aa_cov": 0.9807692307692307, "hmm_cov": 0.9803921568627451, "evalue": 3.28931844933566e-34, "score": 114.4564437866211, "start": 2, "stop": 103 } ], "seq_stats": { "molecular_weight": 11428.000799999985, "isoelectric_point": 9.435479927062985 }, "id": "EFPKPENHBP_274", "locus": "GCA000186005_00234" }, { "type": "cds", "sequence": "contig_1", "start": 129930, "stop": 130187, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGA", "aa": "MNAATIIQLAIYLLPNLLKIPMAIPNILGIITAKVIKFSIIEAIIDLPISFVPWKTRSELIDKLKAITLSVTILINKVVPAALSG", "aa_hexdigest": "38101b8e94f8a3d0d2697a829e0a3953", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.25609999999, "isoelectric_point": 9.777162361145017 }, "id": "EFPKPENHBP_275", "locus": "GCA000186005_00235" }, { "type": "cds", "sequence": "contig_1", "start": 130277, "stop": 130537, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGCCTACTTTTGCCTTAGGTTTTAGTGGTTTCATAAGATCATTTGGTGTTGCTATTTCATTTGCTGTTATGAACATTTTCTTAACCAAAACTCCTTTTGCGGAACCACTTTCATTCCAATATTATTGGTCAAAAGGTATGCCGATTGTTTCATTATTCTTAGTTTCTATTTTTGGTATTAATGATGGAGCTAGAAGCTTAATGTCATATAACTATACGCGAAGAAACTTTAAAAGATGCAAAGAAGTTTATCTTTGA", "aa": "MLPTFALGFSGFIRSFGVAISFAVMNIFLTKTPFAEPLSFQYYWSKGMPIVSLFLVSIFGINDGARSLMSYNYTRRNFKRCKEVYL", "aa_hexdigest": "9d5d739d0015717808804b47359bbafe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9872.533399999991, "isoelectric_point": 9.916414070129392 }, "id": "EFPKPENHBP_276", "locus": "GCA000186005_00236" }, { "type": "cds", "sequence": "contig_1", "start": 130583, "stop": 130687, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTATTAGGTGGTGTTGTTGCTACTATTATTGGTTGGTCAATTAACTTAACTGCCTACTTAGCTTATGTGGTAATTAATAGCAAGCGGAATAAAACCTGA", "aa": "MGVLGGVVATIIGWSINLTAYLAYVVINSKRNKT", "aa_hexdigest": "3d1d62a2394cb888ef13294ae4f8eb2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3623.2691000000004, "isoelectric_point": 9.996161460876465 }, "id": "EFPKPENHBP_277", "locus": "GCA000186005_00237" }, { "type": "cds", "sequence": "contig_1", "start": 131051, "stop": 131317, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCAATTTTTACCAGATACTTTAAACTCTTTAACTTCAAACGAATTGAAGCAGAAATACTTGGATATCATAAATAACTCCAGTTATAAAGATCTGAATTTAGGAAAAATTGATGCTAATGGGGTTGTTTCAATGACCACAGCTGCAATTCAACCTTTAATTATTTTTTCTAACTCAATAGTATTTTTAGTACCTGTTGGTGCAAGTATTTATTACACAAAATGTATTTCTAAAAAATTAGAAAAAAGTGGAAGAGACTTGTGA", "aa": "MIQFLPDTLNSLTSNELKQKYLDIINNSSYKDLNLGKIDANGVVSMTTAAIQPLIIFSNSIVFLVPVGASIYYTKCISKKLEKSGRDL", "aa_hexdigest": "203bcc40dc06ae7e616f1f5464935e5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9728.218799999995, "isoelectric_point": 8.759271049499514 }, "id": "EFPKPENHBP_278", "locus": "GCA000186005_00238" }, { "type": "tRNA", "sequence": "contig_1", "start": 131599, "stop": 131674, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 75.0, "nt": "GCCGTCATAGCTCAGTCGGTAGAGCACATCCATGGTAAGGATGGGGTCGCTGGTTCAAGTCCAGTTGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 131655, 131657 ], "id": "EFPKPENHBP_34", "locus": "GCA000186005_00239" }, { "type": "cds", "sequence": "contig_1", "start": 131924, "stop": 133045, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "RefSeq:WP_013526678.1", "SO:0001217", "UniParc:UPI0001A8C08C", "UniRef:UniRef100_C4XE71", "UniRef:UniRef50_C4XE71", "UniRef:UniRef90_C4XE71" ], "nt": "ATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAGCTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTTAGCAAGTTTACTCAAAATATTTTTGATACAGTATTCAATTTTGGAGGTGGAGCTTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGCGATGATATTTACACTGAAATTGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTTGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATGTTAGAACTTGTAATGTTTGTCAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAA", "aa": "MPKKNYYDVLGVPKTASEREIKTAYRKLAMKYHPDKLKDGTSDQKMQELNEAYEVLSDPTKKSNYDRFGSPDGPRPGQGFGMNFGGEMRDFSKFTQNIFDTVFNFGGGASKQKSSKVEKARGDDIYTEIVVDFIEAVKGKEFKEKLNKFELCSRCKGTGADNPSDVRTCNVCQGKGQKETQTRSVLGYMKMVSTCDKCNGTGKLIGKTCRECNGSLYTKKLKNVTFKIPEGSDSGDKIKIDGYGEKGRNGGESGDLYIVIIVKEHKYFKRNGLDLFLELPVSFLDIVKENSVLVPTPYGFERIKMKNTYQFGKVLNLTGKGIRTKKGTGNLKVALQVVMPNFTSDQYNEMAKILEPYEDNVNAEFSELVKSEK", "aa_hexdigest": "2808ef31d4a480422d963c1a69d9a2ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C08C", "ncbi_nrp_id": "WP_013526678.1", "uniref100_id": "UniRef100_C4XE71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C08C", "RefSeq:WP_013526678.1", "UniRef:UniRef100_C4XE71" ] }, "ips": { "uniref100_id": "UniRef100_C4XE71", "uniref90_id": "UniRef90_C4XE71" }, "psc": { "uniref90_id": "UniRef90_C4XE71", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_C4XE71" }, "pscc": { "uniref50_id": "UniRef50_C4XE71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE71" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "EFPKPENHBP_279", "locus": "GCA000186005_00240" }, { "type": "cds", "sequence": "contig_1", "start": 133082, "stop": 133318, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72", "UniRef:UniRef90_C4XE72" ], "nt": "ATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGA", "aa": "MKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKK", "aa_hexdigest": "7eb248a538f98237d26455d7d21a1f52", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 133079, "stop": 133558, "observations": { "mutation": [ 133316 ], "stop": [ 133316 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE72", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 1.0, "score": 152.0, "evalue": 8.01e-46, "gene_start": 1, "gene_end": 78, "reference_start": 2, "reference_end": 79, "reference_sequence": "MMKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKKWIDLKNKVDSYDTQNFDLSNNFFKKMSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL" }, "paralog": false, "identity": 0.9874213836477987, "score": 315.0, "evalue": 1.31e-112, "description": "Internal stop codon at 133316. Nonsense mutation around 133316.", "psc": { "uniref90_id": "UniRef90_C4XE72", "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE72", "UniRef:UniRef50_C4XE72" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9479.60649999999, "isoelectric_point": 5.366135215759278 }, "id": "EFPKPENHBP_280", "locus": "GCA000186005_00241" }, { "type": "cds", "sequence": "contig_1", "start": 133391, "stop": 133558, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAA", "aa": "MSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL", "aa_hexdigest": "4d58b6faf28baa5f09a76ea62af54572", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6282.857500000002, "isoelectric_point": 4.286308097839356 }, "id": "EFPKPENHBP_281", "locus": "GCA000186005_00242" }, { "type": "cds", "sequence": "contig_1", "start": 133677, "stop": 133823, "strand": "+", "frame": 3, "gene": null, "product": "4'-phosphopantetheinyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8", "UniRef:UniRef90_C4XE73" ], "nt": "ATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGA", "aa": "MIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAI", "aa_hexdigest": "d87b1d34bf5650a801bdbba8e7e3782f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 133665, "stop": 133985, "observations": { "mutation": [ 133821, 133905 ], "stop": [ 133821, 133905 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE73", "query_cov": 1.0, "subject_cov": 0.4528301886792453, "identity": 1.0, "score": 95.9, "evalue": 1.1e-24, "gene_start": 1, "gene_end": 48, "reference_start": 5, "reference_end": 52, "reference_sequence": "MIRTMIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAIWAIKEAMFKVDNSLFEFKNIKLQKENESWQHKNFDISISHEDDYLVAVVLKKKE" }, "paralog": false, "identity": 0.9716981132075472, "score": 199.0, "evalue": 4.22e-68, "description": "Internal stop codon at 133821, 133905. Nonsense mutation around 133821, 133905.", "psc": { "uniref90_id": "UniRef90_C4XE73", "product": "4'-phosphopantetheinyl transferase domain-containing protein", "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE73", "UniRef:UniRef50_D5E5U8" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "EFPKPENHBP_282", "locus": "GCA000186005_00243" }, { "type": "cds", "sequence": "contig_1", "start": 134206, "stop": 134604, "strand": "+", "frame": 1, "gene": null, "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74", "UniRef:UniRef90_C4XE74" ], "nt": "TTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGA", "aa": "MIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNR", "aa_hexdigest": "23c9f92da5bd43a2c14388764de830ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 133960, "stop": 134733, "observations": { "mutation": [ 133969, 134020, 134026, 134032, 134113, 134602 ], "stop": [ 133969, 134020, 134026, 134032, 134113, 134602 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE74", "query_cov": 1.0, "subject_cov": 0.5136186770428015, "identity": 0.992, "score": 254.0, "evalue": 1.2e-83, "gene_start": 1, "gene_end": 132, "reference_start": 83, "reference_end": 214, "reference_sequence": "MWFWKRRSNMNYHLKMFLTWWIWLWRIYRISVYARKYRHTPDYYTPQQRYDWLLKKVKKYLKLYNIKVTVEGYDNLPKAPCLLIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNRWKKQEFKVKFLNPIKPSTFMSMDPGALADRVKSLMERELENES" }, "paralog": false, "identity": 0.9727626459143969, "score": 491.0, "evalue": 1.29e-179, "description": "Internal stop codon at 133969, 134020, 134026, 134032, 134113, 134602. Nonsense mutation around 133969, 134020, 134026, 134032, 134113, 134602.", "psc": { "uniref90_id": "UniRef90_C4XE74", "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE74", "UniRef:UniRef50_C4XE74" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74" ], "product": "Phospholipid/glycerol acyltransferase domain-containing protein" } }, "id": "EFPKPENHBP_283", "locus": "GCA000186005_00244" }, { "type": "cds", "sequence": "contig_1", "start": 134723, "stop": 135481, "strand": "+", "frame": 2, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "RefSeq:WP_013354376.1", "SO:0001217", "UniParc:UPI0001A8C090", "UniRef:UniRef100_C4XE75", "UniRef:UniRef50_A0A449B1R9", "UniRef:UniRef90_C4XE75" ], "nt": "ATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGATATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAA", "aa": "MKASLYATDDHKYDIKLENFDGPLDLLLALVQEKNVDIMDVDVSELATAYLKIIQNLQEHEIDIAGDYLVMAATLLALKTKMLLFTPEEKPEIEEDKREILRRLYEYQQFKEISQTLRQQEALRHEIFIKSPSDVEEFMVDDDSTALDGASNPLKLITTLRKMFERTYAMQLRRTKLETFNLTPKDQIPFILNLFDHCDTVTFEMIFKQPSLNHFVITLLAVLDLSRRQIIILKQNEQFGTITFEKGPEYEK", "aa_hexdigest": "74a996ae10002adb3c97e3bb0b11aa93", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C090", "ncbi_nrp_id": "WP_013354376.1", "uniref100_id": "UniRef100_C4XE75", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C090", "RefSeq:WP_013354376.1", "UniRef:UniRef100_C4XE75" ] }, "ips": { "uniref100_id": "UniRef100_C4XE75", "uniref90_id": "UniRef90_C4XE75" }, "psc": { "uniref90_id": "UniRef90_C4XE75", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_A0A449B1R9", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_A0A449B1R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B1R9" ], "product": "Segregation and condensation protein A" }, "genes": [], "id": "EFPKPENHBP_284", "locus": "GCA000186005_00245" }, { "type": "cds", "sequence": "contig_1", "start": 135471, "stop": 136055, "strand": "+", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_013526679.1", "SO:0001217", "UniParc:UPI0001F59D37", "UniRef:UniRef100_C4XE76", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_C4XE76" ], "nt": "ATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACCAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAA", "aa": "MKNKILEALLYVQGDEGLTLIQVKEIFGLNTIAEARKVMNDFIKSYNDADGALKIVNFNEVYKLATRETYKEYITKMVQVVKKHRLSNAAIEVAGITAYKQPVTRSMINNIRGVQSDQVLNSLLTKGVIEEVGISPTPGNPILYGITNKFYDYFKIKSLAELPKLTEFNFVDGVENEAEDFNLFDSQRPENYDN", "aa_hexdigest": "7ddafeacf8dce10e28c72552807be4df", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F59D37", "ncbi_nrp_id": "WP_013526679.1", "uniref100_id": "UniRef100_C4XE76", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D37", "RefSeq:WP_013526679.1", "UniRef:UniRef100_C4XE76" ] }, "ips": { "uniref100_id": "UniRef100_C4XE76", "uniref90_id": "UniRef90_C4XE76" }, "psc": { "uniref90_id": "UniRef90_C4XE76", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "EFPKPENHBP_285", "locus": "GCA000186005_00246" }, { "type": "cds", "sequence": "contig_1", "start": 136109, "stop": 136948, "strand": "+", "frame": 2, "gene": null, "product": "RNA-binding S4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96", "UniRef:UniRef90_C4XE77" ], "nt": "ATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTAGCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGTGACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACAGGTTTTTTTGAAAAAATATATAAATTAAAAAGTGATTTTAATGAAGTTAAAAAACTTGTAGAAATTAATATTCAGCATGATGAAAAATTACACAAAATGGTTGTTACAAATAATGCTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATGAAATTTTTGCATCTATTTTAACTGGCAAAAAACATCAAATTAGATTAAGCATGTCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGACGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGA", "aa": "MLEFIATKNDDGRKLISYLKRILINTPTSHIYKILRNKDIKINGVRINDPQYIINEKDIIQIYGIEANDYQNKKFENSAKKDFKIIYEDDNILIVSKPINLAVHSENNCLDKQVLSYLKYNQKDSFKPSHVGRLDKVTSGLMIYAKNYATLVELNYKTGFFEKIYKLKSDFNEVKKLVEINIQHDEKLHKMVVTNNAKDPLAKTLFYTKNNEIFASILTGKKHQIRLSMSHLKTPIYGDIKYDGKKADRLYLHCYSITFKNLDNSLKYLNNKEFNDPIN", "aa_hexdigest": "363e4e2f86aef649538d891c8121866f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XE77", "query_cov": 1.0, "subject_cov": 0.9425675675675675, "identity": 1.0, "score": 556.0, "evalue": 7.36e-200, "valid": true, "product": "RNA-binding S4 domain-containing protein", "uniref50_id": "UniRef50_A0A449AI96" }, "pscc": { "uniref50_id": "UniRef50_A0A449AI96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [], "id": "EFPKPENHBP_286", "locus": "GCA000186005_00247" }, { "type": "cds", "sequence": "contig_1", "start": 136960, "stop": 137055, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGACCAAATTATGACTGAATGA", "aa": "MKKVTKEELKKIVASLMLEPTNEVLDQIMTE", "aa_hexdigest": "cd56f7c12800aee7ae9f920db93b2950", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3590.2747, "isoelectric_point": 5.232790946960448 }, "id": "EFPKPENHBP_287", "locus": "GCA000186005_00248" }, { "type": "cds", "sequence": "contig_1", "start": 137080, "stop": 137262, "strand": "+", "frame": 1, "gene": null, "product": "Glutamyl-tRNA amidotransferase subunit C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61", "UniRef:UniRef50_UPI0021AE6C6E", "UniRef:UniRef90_C4XE78" ], "nt": "ATGCTTAAAAAATTAAATACAACAAATGTTAAACCATTAAGTCACATCAATGAAGAAGCTTTAGTAGACTTCTTAAGAGAAGATGTTGAAGACACTTCTTATAGTATCAACAAACAAACTGCATTAAACAATGCTATGGACAAAGATCAAGATTACATTATTACAACAAAGGTGGTGAAATAA", "aa": "MLKKLNTTNVKPLSHINEEALVDFLREDVEDTSYSINKQTALNNAMDKDQDYIITTKVVK", "aa_hexdigest": "8a18ba8f4a1d0b68e9f1595b3dce1efd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AE6C6E", "query_cov": 0.9333333333333333, "subject_cov": 1.0, "identity": 0.518, "score": 57.8, "evalue": 6.02e-10, "valid": false, "product": "Glu-tRNAGln amidotransferase C subunit", "uniref50_id": "UniRef50_UPI0021AE6C6E" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AE6C6E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AE6C6E" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 136945, "stop": 137262, "observations": { "mutation": [ 137053 ], "stop": [ 137053 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE78", "query_cov": 1.0, "subject_cov": 0.5714285714285714, "identity": 1.0, "score": 117.0, "evalue": 7.51e-33, "gene_start": 1, "gene_end": 60, "reference_start": 46, "reference_end": 105, "reference_sequence": "MIRRIMKKVTKEELKKIVASLMLEPTNEVLDQIMTEWKVLNKNLNMLKKLNTTNVKPLSHINEEALVDFLREDVEDTSYSINKQTALNNAMDKDQDYIITTKVVK" }, "paralog": false, "identity": 0.9809523809523809, "score": 199.0, "evalue": 1.24e-67, "description": "Internal stop codon at 137053. Nonsense mutation around 137053.", "psc": { "uniref90_id": "UniRef90_C4XE78", "product": "Glutamyl-tRNA amidotransferase subunit C", "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE78", "UniRef:UniRef50_D4XV61" ] }, "pscc": { "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61" ], "product": "Glutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amidotransferase, C subunit" } }, "id": "EFPKPENHBP_288", "locus": "GCA000186005_00249" }, { "type": "cds", "sequence": "contig_1", "start": 137262, "stop": 138164, "strand": "+", "frame": 3, "gene": null, "product": "amidase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "nt": "ATGAAACTACAAGTCTTAGGTGATTTTGATTTAGCCCAAGCTGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGCTTTAAAAGAAGGCGATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCAACTAAAGATGCAAAAACAACAGCTTCGAGCTTAATTTTAGAAAACTTCAAACCTGGCTACAATGCAACCTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAATAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGA", "aa": "MKLQVLGDFDLAQAELKKNKNNAVAYVYDKALKEGDGLLNNAVFTIKEVFATKDAKTTASSLILENFKPGYNATCVQKLIDAGALIVAKVYCDELALGGTGTFSAFGLIKNPLDKERLAGGSSSGSVATFTDNISFALGSDTGDSVRLPASYNGNVGFKPSYGAISRYGMFAYASSLDTVSYFAHNVNDIAVISQVLFGVDNKDFTSKKVKIDNVQKLQPKKIGVLNVSKNLSNFVSNNFQELIVKLKANKNIKVEVIEPQEDLLNAVKAIYDIISYSEASSNLANLNGIAFGSRKEGES", "aa_hexdigest": "b7baf40c1ef36ba7cfcb134b2f427f7f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D5FF4", "query_cov": 0.83, "subject_cov": 0.9841897233201581, "identity": 0.51, "score": 230.0, "evalue": 1.47e-71, "valid": false, "product": "amidase family protein", "uniref50_id": "UniRef50_UPI00215D5FF4" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5FF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "product": "amidase family protein" }, "genes": [], "id": "EFPKPENHBP_289", "locus": "GCA000186005_00250" }, { "type": "cds", "sequence": "contig_1", "start": 138174, "stop": 138314, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGA", "aa": "MTNTRSQGFGKMVQRRLTLGTFFLHSENQKDLFLKAQQGRRLFKEY", "aa_hexdigest": "c4269d1ec5cc4fa248b92c99039a2fde", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5508.303, "isoelectric_point": 11.000063133239745 }, "id": "EFPKPENHBP_290", "locus": "GCA000186005_00251" }, { "type": "cds", "sequence": "contig_1", "start": 138321, "stop": 138467, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGA", "aa": "MHKQYDIVIYPAYADVAPYINKKNKNYKYMEYILTGSNLAGNPSITIP", "aa_hexdigest": "42b402ce2ced37fc65c407ffb3b23ffb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.311700000001, "isoelectric_point": 8.964216041564942 }, "id": "EFPKPENHBP_291", "locus": "GCA000186005_00252" }, { "type": "cds", "sequence": "contig_1", "start": 138577, "stop": 139464, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002963D433" ], "nt": "ATGAATAATTTTGAAGTAATTATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATAATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGA", "aa": "MNNFEVIIGIEIHLELNTKTKMFSPARIDFNAEPNTTMNQIDLGYPGTLPLVNKEAVLSGIKLAKALNMKIDDELHFDRKNYFYPDLPKGYQITQFLRPIGSEGYVTINTDKGPKKIQIERIHLEEDTARQHHDEEGTKLDYNRAGVPLIEIVTHPVLRSADEAVAYVDMIRKIALSLNISDAKMEQGSLRADVNVSLRPYGYKGFGTKVEIKNMNSFRAIKNALEFEIDFQRKQILTNKPILQQTKRYNEATLSTVVMRTKTGEVDYKYFPEPNIPFIKLSKKLIDSVKLNELP", "aa_hexdigest": "d6148eafd332b0aedca401f8909b698b", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002963D433", "query_cov": 0.9830508474576272, "subject_cov": 0.8787878787878788, "identity": 0.541, "score": 298.0, "evalue": 2.51e-97, "valid": false, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "uniref50_id": "UniRef50_UPI002963D433", "go_ids": [ "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002963D433", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002963D433" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB" }, "genes": [ "gatB" ], "id": "EFPKPENHBP_292", "locus": "GCA000186005_00253" }, { "type": "cds", "sequence": "contig_1", "start": 139690, "stop": 139827, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGA", "aa": "MDQDIISGRSFKKLVPLLVNFDGDFDKLVVDNNLKQISDEATITK", "aa_hexdigest": "61620e15f46e48a5677bbd62617ae68e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 44, "aa_cov": 0.9777777777777777, "hmm_cov": 1.0, "evalue": 1.715557308439862e-07, "score": 27.535066604614258, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5081.7497, "isoelectric_point": 4.609039878845214 }, "id": "EFPKPENHBP_293", "locus": "GCA000186005_00254" }, { "type": "cds", "sequence": "contig_1", "start": 140128, "stop": 140715, "strand": "+", "frame": 1, "gene": null, "product": "Abortive infection protein AbiGI", "db_xrefs": [ "RefSeq:WP_013354381.1", "SO:0001217", "UniParc:UPI0001A8C096", "UniRef:UniRef100_C4XE81", "UniRef:UniRef50_C4XE81", "UniRef:UniRef90_C4XE81" ], "nt": "ATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAA", "aa": "MQEEKVIKIGKKHNGVITTKECAMQRIHNQTLHRLLSKKIIEKISHGIYLLTEYLEDDHYLFQLRYPKTIFSYETALYLIHETDFLPINFDVSVTKGYKFNDKENIRVNYVSDELADLGKIKVKTMFNNDVYVYCYERVLCDLIKNRKKANLETYVNAIKRYAQYKNKNLDLLYEIAKKMNILDEMKNIMIGIIY", "aa_hexdigest": "0f07e925844bbe441d709750411fb93b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C096", "ncbi_nrp_id": "WP_013354381.1", "uniref100_id": "UniRef100_C4XE81", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C096", "RefSeq:WP_013354381.1", "UniRef:UniRef100_C4XE81" ] }, "ips": { "uniref100_id": "UniRef100_C4XE81", "uniref90_id": "UniRef90_C4XE81" }, "psc": { "uniref90_id": "UniRef90_C4XE81", "product": "Abortive infection protein AbiGI", "uniref50_id": "UniRef50_C4XE81" }, "pscc": { "uniref50_id": "UniRef50_C4XE81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE81" ], "product": "Abortive infection protein AbiGI" }, "genes": [], "id": "EFPKPENHBP_294", "locus": "GCA000186005_00255" }, { "type": "cds", "sequence": "contig_1", "start": 140717, "stop": 140902, "strand": "+", "frame": 2, "gene": null, "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82", "UniRef:UniRef90_C4XE82" ], "nt": "ATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGA", "aa": "MTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYK", "aa_hexdigest": "ce0a3ea49624b32cb75c3919e102d53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 140711, "stop": 141034, "observations": { "mutation": [ 140900 ], "stop": [ 140900 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE82", "query_cov": 1.0, "subject_cov": 0.5700934579439252, "identity": 1.0, "score": 120.0, "evalue": 5.02e-34, "gene_start": 1, "gene_end": 61, "reference_start": 3, "reference_end": 63, "reference_sequence": "MNMTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYKWRTTVDLDFMSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKT" }, "paralog": false, "identity": 0.9813084112149533, "score": 209.0, "evalue": 7.75e-72, "description": "Internal stop codon at 140900. Nonsense mutation around 140900.", "psc": { "uniref90_id": "UniRef90_C4XE82", "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE82", "UniRef:UniRef50_C4XE82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82" ], "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein" } }, "id": "EFPKPENHBP_295", "locus": "GCA000186005_00256" }, { "type": "cds", "sequence": "contig_1", "start": 140927, "stop": 141034, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACATAA", "aa": "MSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKT", "aa_hexdigest": "4ec8de24b34104968cd2f97803fa8802", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4173.783000000001, "isoelectric_point": 8.007634162902832 }, "id": "EFPKPENHBP_296", "locus": "GCA000186005_00257" }, { "type": "cds", "sequence": "contig_1", "start": 141071, "stop": 141265, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGA", "aa": "MYVKVKIKNITEIVPLDIVEESKIDPTYISKEFNSIYGENFTFLIYNIENVIAEKVFAIYTNRF", "aa_hexdigest": "bfc267f768b0b7193ae3276e881156a2", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7569.673999999996, "isoelectric_point": 4.9316011428832995 }, "id": "EFPKPENHBP_297", "locus": "GCA000186005_00258" }, { "type": "cds", "sequence": "contig_1", "start": 141794, "stop": 142087, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTAGTTGAAAAATATATTCAACAAGCTGGATATGTTGAAAACAAAACATATTTTAAACAAATATATTCAAGTGAGATCTATGAAAAGTTTGGAATTAATTTAAATAATATTCAAGAAACAACTGCAAATAAAAGATTTGATTTTGGAATCATTAAAAATCATAAATTTTATGCAATTGAAGTAAATTTTTATACGGCTCAAGGTTCTAAACTTAATGAAGTTTCAAGAAGCTATGAAAAAATTTATTTATCTACAAAAACAATTACTAATTTTGAATTCATTTGA", "aa": "MEKLVEKYIQQAGYVENKTYFKQIYSSEIYEKFGINLNNIQETTANKRFDFGIIKNHKFYAIEVNFYTAQGSKLNEVSRSYEKIYLSTKTITNFEFI", "aa_hexdigest": "f4fe23e10362a9884fb675aa0d21918a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11564.959199999988, "isoelectric_point": 8.730066871643068 }, "id": "EFPKPENHBP_298", "locus": "GCA000186005_00259" }, { "type": "cds", "sequence": "contig_1", "start": 142530, "stop": 143579, "strand": "-", "frame": 2, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "RefSeq:WP_041594275.1", "SO:0001217", "UniParc:UPI0002ECF00F", "UniRef:UniRef100_C4XE85", "UniRef:UniRef50_A0A1V5HP79", "UniRef:UniRef90_C4XE85" ], "nt": "ATGGAAACACTTTTTAAAACAATTGGCGACAATATTAAAATGGAAAGACTTGCAAAAAATATGACACAACAAGAATTAGCAGAATTAAGTAGCACCGAAAGATCTCAATTAACTAAAATTGAAAAAGGTCAAATTAAGGGCGTTCAGTTTTTTACAATTTGCAAAATATATAATGCTTTGAATTTGAATAATATTTATATTGGAAAATTAAACACAAAACTTTCACCATTTGTAAAATGGGCTGGTGGCAAAACTCAATTATTAGATAAACTTAAAAGTTTAATGCCAAAAACATATAACAATTATTTGGAACCTTTTGTTGGTGGCGGAGCATTATTACTAAATCTAAAGCCGCAAAATTTCATAATAAATGATTTTAATAAAGAATTAATTAATGTTTTTAATTGCTTTAAAAATGATAAAGATTTTAAATTATTAAAAAAAGAATTAATTAATCATGAAAACAATCATAATGATGGGTATTACTATAAAATAAGAGATTTGGACAAATTAGATAAATACGATAAACTGCCAAATTACAAAAAAGCTGCCCGAACAATATACTTAAATAAAGCATGTTTTAATGGTTTATATCGAGTAAATTCTAAAGGACAATTTAATGTACCTTCTGGCAAGAAAAACTCAGTTAATTGCTTTGATAGAGACAATTTTGAAAATTTAAAAAACTTTTTAAAAACTACAAATAATCAAATTTTCTGTGAAGATTTTGAAGTATTTTGTGAAAAAGCTCAAAAAGGAGATTTTGTTTATTTTGATCCTCCTTATGATGTCATTGAAAACAAAAATACTTTCACTTCATATAATTCAGAAAGTTTTGGAAAAGAAGAACAAAAACGATTATCCGAAGTTTTTAAAAAACTTGACAAAAAAGGTGTCAAAGTAATGTTAAGTAATCACAACACAAAATATATTAATGAATTATATGCAGACTTTAATATTAATGTTGTTAAAGCAAAAAGAATGATAAATTCAAATGGAAGTGGACGAGGAGAAGTTGAAGAAGTAATAATTACAAATTATAGGGAGTAA", "aa": "METLFKTIGDNIKMERLAKNMTQQELAELSSTERSQLTKIEKGQIKGVQFFTICKIYNALNLNNIYIGKLNTKLSPFVKWAGGKTQLLDKLKSLMPKTYNNYLEPFVGGGALLLNLKPQNFIINDFNKELINVFNCFKNDKDFKLLKKELINHENNHNDGYYYKIRDLDKLDKYDKLPNYKKAARTIYLNKACFNGLYRVNSKGQFNVPSGKKNSVNCFDRDNFENLKNFLKTTNNQIFCEDFEVFCEKAQKGDFVYFDPPYDVIENKNTFTSYNSESFGKEEQKRLSEVFKKLDKKGVKVMLSNHNTKYINELYADFNINVVKAKRMINSNGSGRGEVEEVIITNYRE", "aa_hexdigest": "1d5dc5de2bf46fa4d728d379609e56fe", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002ECF00F", "ncbi_nrp_id": "WP_041594275.1", "uniref100_id": "UniRef100_C4XE85", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002ECF00F", "RefSeq:WP_041594275.1", "UniRef:UniRef100_C4XE85" ] }, "ips": { "uniref100_id": "UniRef100_C4XE85", "uniref90_id": "UniRef90_C4XE85" }, "psc": { "uniref90_id": "UniRef90_C4XE85", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A1V5HP79", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HP79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HP79" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "EFPKPENHBP_299", "locus": "GCA000186005_00260" }, { "type": "cds", "sequence": "contig_1", "start": 143666, "stop": 143836, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13412.12", "PFAM:PF13730.12", "PFAM:PF01978.25" ], "nt": "TTGCCAGATTCTGCTTATTATGTATATATGGCTTTAAAAAATAATCCAAAAGAAACAGCTGGTTCACTAGCTAAAAAAATAAAAAAATCTTCGAGAACTATTCAAAGAATTTTAGATCTTTTAAAAGACAAGGGCTACATAGCTAGAATTGGTAAGACAAGAGGATATTGA", "aa": "MPDSAYYVYMALKNNPKETAGSLAKKIKKSSRTIQRILDLLKDKGYIARIGKTRGY", "aa_hexdigest": "7ef3bf57f3b285762e7fce32fe31178b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13412.12", "name": "HTH_24", "length": 37, "aa_cov": 0.6607142857142857, "hmm_cov": 1.0, "evalue": 1.8518021203578027e-06, "score": 23.758155822753906, "start": 11, "stop": 47 }, { "id": "PF13730.12", "name": "HTH_36", "length": 35, "aa_cov": 0.5714285714285714, "hmm_cov": 1.0, "evalue": 3.234606193648088e-07, "score": 26.415742874145508, "start": 16, "stop": 47 }, { "id": "PF01978.25", "name": "TrmB", "length": 45, "aa_cov": 0.8035714285714286, "hmm_cov": 1.0, "evalue": 5.163869127958328e-07, "score": 25.81563377380371, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6365.430400000001, "isoelectric_point": 10.118457984924316 }, "id": "EFPKPENHBP_300", "locus": "GCA000186005_00261" }, { "type": "cds", "sequence": "contig_1", "start": 143837, "stop": 144016, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTAGTTGATGAAGCTAAAATTTGTAATAATGGTTTCAAGAGAGTATATGAAGAATGTGAAAAAACTAATGCTAAAATTACTTATCAAAAAGGCAATTATGGTTTTGCATTTATATTTTTAAGAGAAAACAAAAGTTTAATTAGTAAAGATGAAGAATTTAAAGAATACTGA", "aa": "MINLVDEAKICNNGFKRVYEECEKTNAKITYQKGNYGFAFIFLRENKSLISKDEEFKEY", "aa_hexdigest": "415fb4a15b0c0f217fed02bb453cd76a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7021.913099999998, "isoelectric_point": 6.222414588928221 }, "id": "EFPKPENHBP_301", "locus": "GCA000186005_00262" }, { "type": "cds", "sequence": "contig_1", "start": 144260, "stop": 144445, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGAAAAACTAACAAATGCTGCTCATTATCTTTTTTCGCATAGAGAACCTATAGTTTTAAAAATGGCTGTTTTTGCAACAGATCAAAAATTAACATTTTTAGATATCAATAGAATTTCTGGTAACATCTTTCAATTAATTGAGAAAGCTAATGAATATGTTAAATCTAAAATCAATTGA", "aa": "MKNEKLTNAAHYLFSHREPIVLKMAVFATDQKLTFLDINRISGNIFQLIEKANEYVKSKIN", "aa_hexdigest": "f8413889cba5f0f0837b8715b90f8b0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7096.193599999999, "isoelectric_point": 9.455529594421389 }, "id": "EFPKPENHBP_302", "locus": "GCA000186005_00263" }, { "type": "cds", "sequence": "contig_1", "start": 144584, "stop": 144982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04326.19" ], "nt": "ATGAATTTTGGAATCGAAAGCGAAACACTTGAATTTAAGAAAAATACAAATGAAATAAATGAGGCTATGAAATCAATTTGTGCCATGTTAAATAAACACGGAAAAGGCACATTATATTTTGGCATCGGAGATGATGGATTAGTATATGATCAAAAAATCAACATAAATACTTTAAGAGATATTTGCAGAAAAATTTATGAAAATATTGAACCTGTAATTAGTCCTATTATTGAGAAAAAAATTGTTGATAATAAACAAATAATAGAGGTAACTTTTTGTGGGAATGATGCTCCATATTCAGTTGAAGGCATCTATTATATTAGAAATGATGATGAGGATAAAATACTCAGTCCTAATCAATTAAGACAGCTTTTTGAATTCAATAAAAACAAAAGCTGA", "aa": "MNFGIESETLEFKKNTNEINEAMKSICAMLNKHGKGTLYFGIGDDGLVYDQKININTLRDICRKIYENIEPVISPIIEKKIVDNKQIIEVTFCGNDAPYSVEGIYYIRNDDEDKILSPNQLRQLFEFNKNKS", "aa_hexdigest": "c41e6403b0996c4f92f8cefef0c78054", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04326.19", "name": "SLFN_AlbA_2", "length": 117, "aa_cov": 0.8333333333333334, "hmm_cov": 0.9914529914529915, "evalue": 4.493733346123219e-21, "score": 71.92584991455078, "start": 6, "stop": 115 } ], "seq_stats": { "molecular_weight": 15230.18459999999, "isoelectric_point": 5.072220802307128 }, "id": "EFPKPENHBP_303", "locus": "GCA000186005_00264" }, { "type": "cds", "sequence": "contig_1", "start": 145131, "stop": 145418, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTTATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGA", "aa": "MKQYKNIKEVEEKYTFDLEDILKGQTIYDLFNEFIELSKIALKTKDTKYENVENYIKSVELGKDMGVLSNRIHNYLSNHSNQNLVDSKWTDLSTK", "aa_hexdigest": "570e72111223cc751911b6ccbc54df73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11240.561299999988, "isoelectric_point": 5.662323188781738 }, "id": "EFPKPENHBP_304", "locus": "GCA000186005_00265" }, { "type": "cds", "sequence": "contig_1", "start": 145596, "stop": 146174, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACGTGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGA", "aa": "MNQSSSANPDYESIFNIISDSELDYGFATDSKGKKYKLSPAIKAKFMKKDDFKIRKSTRDSFLKAHMKHKDSMANLLYQHFKGLTVTAKIRKYKNTIDMLTHSDKVDDSMLQFLFDKVSTLKHTIKNRNKYYKKFYEAKFKEKYHAKYDSYRELVKVKSTYTVEQMQNIVSEALKPFGSEYHKMITKAINER", "aa_hexdigest": "a42a758137a74d58059930eb56efc4a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22652.824700000012, "isoelectric_point": 9.68361873626709 }, "id": "EFPKPENHBP_305", "locus": "GCA000186005_00266" }, { "type": "cds", "sequence": "contig_1", "start": 146184, "stop": 146522, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGACGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGA", "aa": "MTVDNKLSGAYSIGNTYGLDKKYILMNFDGELGSVETLAHELGHSMHSYFSDKNNDISNASYPIILAEIASIFNELMLYNYLLKTSKNDLFKFKILDNMIDGFVGTVFRQIL", "aa_hexdigest": "4883482d946ef3c0ce0354fe6dd4bc16", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12672.344699999985, "isoelectric_point": 5.1619695663452125 }, "id": "EFPKPENHBP_306", "locus": "GCA000186005_00267" }, { "type": "cds", "sequence": "contig_1", "start": 146616, "stop": 146921, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGA", "aa": "MKYATKKIKYKKEKNIMSVYVPHYYYGFYVYKYAIGQLVANYFYTRVKNEGEKYLQVYINDFLSAGDRDYPLETLKKVGADLKDPQFYEIGFSYFKEIVKE", "aa_hexdigest": "4d2b96b4d73b5534a500c5b01b53f353", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12170.87519999999, "isoelectric_point": 9.17128849029541 }, "id": "EFPKPENHBP_307", "locus": "GCA000186005_00268" }, { "type": "cds", "sequence": "contig_1", "start": 147264, "stop": 147404, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGA", "aa": "MNLIFAFRNQKRYKRDFADFILKKYKKATKAAFLPFLISTLAFYLA", "aa_hexdigest": "e553bb910996a9e17b526de4f0921ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.583899999999, "isoelectric_point": 10.354412269592284 }, "id": "EFPKPENHBP_308", "locus": "GCA000186005_00269" }, { "type": "cds", "sequence": "contig_1", "start": 147423, "stop": 147557, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTATTTAGGTTACATTCCTATTGGGCATACTCAAATAACCTTTTTAGCTTTTATAGTTGTTTTTGGAACTTTTCACTTAGATTATGTTGGTAATCTTTCAGCAGGATTATGTTTTGGATTTAGCTCATGA", "aa": "MPYLGYIPIGHTQITFLAFIVVFGTFHLDYVGNLSAGLCFGFSS", "aa_hexdigest": "ab97a21f85ac26d91f15f5148104965b", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4815.563399999999, "isoelectric_point": 5.926397132873535 }, "id": "EFPKPENHBP_309", "locus": "GCA000186005_00270" }, { "type": "cds", "sequence": "contig_1", "start": 147699, "stop": 147809, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATAAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGA", "aa": "MISIIAAILNIVLTLSFQHLHNKIFGDLKNLLPIKE", "aa_hexdigest": "cda994597b2b724a3f34ab4257e347c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4071.911, "isoelectric_point": 8.296323585510255 }, "id": "EFPKPENHBP_310", "locus": "GCA000186005_00271" }, { "type": "cds", "sequence": "contig_1", "start": 148335, "stop": 148532, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGA", "aa": "MKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIED", "aa_hexdigest": "378f1d9c066f4018d6a3a668c29f5142", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7653.0238999999965, "isoelectric_point": 7.911253929138184 }, "id": "EFPKPENHBP_311", "locus": "GCA000186005_00272" }, { "type": "cds", "sequence": "contig_1", "start": 148899, "stop": 149612, "strand": "+", "frame": 3, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_C4XE89", "UniRef:UniRef90_C4XE89" ], "nt": "ATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTGTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAA", "aa": "MSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEALSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN", "aa_hexdigest": "fd79a4319fcf278f90f1744197601759", "start_type": "ATG", "rbs_motif": null, "genes": [ "tig" ], "truncated": "5-prime", "pseudogene": { "start": 148599, "stop": 149612, "observations": { "mutation": [ 148743 ], "stop": [ 148743 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE89", "query_cov": 1.0, "subject_cov": 0.4637964774951076, "identity": 0.996, "score": 421.0, "evalue": 4.34e-144, "gene_start": 1, "gene_end": 237, "reference_start": 275, "reference_end": 511, "reference_sequence": "MRRIMAENNKKFTIDEKKSELVYTLEINGKEWEKLYADAKAKEVKNIKIPGFRPGKAPQHEIEKRVNPLSVASEVVDQYWRNNHDRLKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIEDWIAQQLEKNALLLPLKKDEKTKDGDTVTIDYKGFIKDEPFDGGEAQGFDLKLGSHTFIDTFEDQLIGKKIGWKGEVKVTFPKEYAVEKLKGQPATFEVEIKDAKRREKVELTDKNFGTVRLAKMSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEAFSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN" }, "paralog": false, "identity": 0.9940652818991098, "score": 651.0, "evalue": 3.94e-237, "description": "Internal stop codon at 148743. Nonsense mutation around 148743.", "psc": { "uniref90_id": "UniRef90_C4XE89", "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_C4XE89", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE89", "EC:5.2.1.8", "UniRef:UniRef50_C4XE89", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE89" ], "product": "Trigger factor" } }, "id": "EFPKPENHBP_312", "locus": "GCA000186005_00273" }, { "type": "cds", "sequence": "contig_1", "start": 149739, "stop": 150047, "strand": "+", "frame": 3, "gene": null, "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "nt": "ATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCGTATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGA", "aa": "MLNIVLFEPEISPNTGNIIRTCFALGAKLHIIKPIGFDLDPKYLSRPAAGRLLSDIQHEVHASYKDFYEKYKNKNIFYLTRYGLKTYTDVDFKKEYKKNKEV", "aa_hexdigest": "22b6a188d1e5174ba9a3513e0f65bdfc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A449AH69", "query_cov": 0.9607843137254902, "subject_cov": 0.9514563106796117, "identity": 0.7240000000000001, "score": 150.0, "evalue": 1.55e-45, "valid": false, "product": "tRNA/rRNA methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A449AH69", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [], "id": "EFPKPENHBP_313", "locus": "GCA000186005_00274" }, { "type": "cds", "sequence": "contig_1", "start": 150051, "stop": 150272, "strand": "+", "frame": 3, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGTTAAGTCTATATGAAGTTCAAAAAGGAAAGGACTTCTTAATCAAATGGAAAAAACAGTAA", "aa": "MFGKESTGIDKEILQTHLENCLRIPMMSEMRSINLANTVAIVGFEIMRQLDFKELSLYEVQKGKDFLIKWKKQ", "aa_hexdigest": "57d40f84f2e8ab4431ba3a570ef850f3", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 0.958904109589041, "subject_cov": 0.8433734939759037, "identity": 0.643, "score": 94.7, "evalue": 4.76e-24, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "EFPKPENHBP_314", "locus": "GCA000186005_00275" }, { "type": "cds", "sequence": "contig_1", "start": 150257, "stop": 150970, "strand": "+", "frame": 2, "gene": "spoU", "product": "SpoU rRNA Methylase family", "db_xrefs": [ "RefSeq:WP_013354390.1", "SO:0001217", "UniParc:UPI0001E32F90", "UniRef:UniRef100_C4XE91", "UniRef:UniRef50_A0A3P8KX10", "UniRef:UniRef90_C4XE91" ], "nt": "ATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAA", "aa": "MEKTVISSVSNPKIKYLKEVQKDNKSDYFLVSGYHLVQEALAKNLVIEIFEEESKSTYKTATLVKSNVLKSISSTMHPDGVVALCLKPKSQIKNKVILLDNVQDPGNVGTIIRNAACFGFDTVISNVNFYNDKVVRATQGALFQVSLIKENNNEDLIKVLNNEGYFIVATSLDKDSIKLDELKINKEKVVLILGNEGNGINKNLYKYCDQKVYIPIEFESLNVAVASGIILNKIYNK", "aa_hexdigest": "49962aab1b0023aa589850a65d2a39c5", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F90", "ncbi_nrp_id": "WP_013354390.1", "uniref100_id": "UniRef100_C4XE91", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F90", "RefSeq:WP_013354390.1", "UniRef:UniRef100_C4XE91" ] }, "ips": { "uniref100_id": "UniRef100_C4XE91", "uniref90_id": "UniRef90_C4XE91" }, "psc": { "uniref90_id": "UniRef90_C4XE91", "product": "SpoU rRNA Methylase family", "uniref50_id": "UniRef50_A0A3P8KX10" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KX10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KX10" ], "product": "tRNA/rRNA methylase" }, "genes": [ "spoU" ], "id": "EFPKPENHBP_315", "locus": "GCA000186005_00276" }, { "type": "cds", "sequence": "contig_1", "start": 150982, "stop": 151083, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGA", "aa": "MKKTNNEKFFNKNFSRELNGYSVPEVDNFIDQL", "aa_hexdigest": "8611b7902e270cc7485b45ba406ab0c1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3967.376000000001, "isoelectric_point": 6.038369941711425 }, "id": "EFPKPENHBP_316", "locus": "GCA000186005_00277" }, { "type": "cds", "sequence": "contig_1", "start": 151280, "stop": 151498, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGA", "aa": "MAKAMREIKENATLADIFLIVLDARIPISSYNEDFDNIAPQKPRLFVITKSDLMDKSKKEKIQKRFQNEKLL", "aa_hexdigest": "8eca640255274886bdac0eb287ae0541", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8410.785899999999, "isoelectric_point": 9.345675468444824 }, "id": "EFPKPENHBP_317", "locus": "GCA000186005_00278" }, { "type": "cds", "sequence": "contig_1", "start": 151751, "stop": 152056, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGA", "aa": "MDTPGILLPKHEDQSAAIKLAMIGSIETNIFPQRFLAENFLKVIVKYYPNKIKEEFNIDIESKDDLSETEAYNIVAQIADNKNFKVDKKIDFDRTYKYILN", "aa_hexdigest": "4bd537d463d1bbe23bd18d8bac485910", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11745.310899999982, "isoelectric_point": 5.1215002059936525 }, "id": "EFPKPENHBP_318", "locus": "GCA000186005_00279" }, { "type": "cds", "sequence": "contig_1", "start": 152099, "stop": 152287, "strand": "+", "frame": 2, "gene": null, "product": "Phage protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94", "UniRef:UniRef90_C4XE94" ], "nt": "ATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTGAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGA", "aa": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYA", "aa_hexdigest": "d1022672db1a9e878e1e3842c3b10eff", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 152099, "stop": 152530, "observations": { "mutation": [ 152285, 152330 ], "stop": [ 152285, 152330 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE94", "query_cov": 1.0, "subject_cov": 0.43356643356643354, "identity": 1.0, "score": 111.0, "evalue": 4.72e-30, "gene_start": 1, "gene_end": 62, "reference_start": 1, "reference_end": 62, "reference_sequence": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYAWQNKAAPSITYTTLLWILICVFSILTILSLYLLILFFIEYSLIKKIGLKKSEQEIEASIRKFVKFGFKKYPKKQMEMLEKF" }, "paralog": false, "identity": 0.986013986013986, "score": 269.0, "evalue": 7.49e-95, "description": "Internal stop codon at 152285, 152330. Nonsense mutation around 152285, 152330.", "psc": { "uniref90_id": "UniRef90_C4XE94", "product": "Phage protein", "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE94", "UniRef:UniRef50_C4XE94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94" ], "product": "Phage protein" } }, "id": "EFPKPENHBP_319", "locus": "GCA000186005_00280" }, { "type": "cds", "sequence": "contig_1", "start": 152656, "stop": 153351, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter related protein", "db_xrefs": [ "RefSeq:WP_013526690.1", "SO:0001217", "UniParc:UPI0001F59E82", "UniRef:UniRef100_A0AB32XAQ9", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_A0AB32XAQ9" ], "nt": "ATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTCAAAACATTGCATTATTAGGATCAAATGGAGCTGGTAAAACCACAACAGTTGAAATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAACTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGTCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATAA", "aa": "MPKLTETQETRMSNSIKEFVENLEKHENRILNHPAIEPREYDGLEKLIEIKGLCKEYKIKGKKFRAVDDMNLTLYKSQNIALLGSNGAGKTTTVEMLVGILKPSAGNIKYFFGDSMDKGKIDATKIGIQFQDSSYPQGLTVRDVIKSMNKIYGNKMKGLELDYLIKIFGVDEYIDNKAASLSGGQHQRLNALLALIHKPELVILDELSTGLDIKIKTRLVEFINNFVKDNK", "aa_hexdigest": "4b2b9c0106e116549dd6251a84605b05", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59E82", "ncbi_nrp_id": "WP_013526690.1", "uniref100_id": "UniRef100_A0AB32XAQ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E82", "RefSeq:WP_013526690.1", "UniRef:UniRef100_A0AB32XAQ9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAQ9", "uniref90_id": "UniRef90_A0AB32XAQ9" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAQ9", "product": "ABC transporter related protein", "uniref50_id": "UniRef50_D1J8M4" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_320", "locus": "GCA000186005_00281" }, { "type": "cds", "sequence": "contig_1", "start": 153358, "stop": 153507, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "RefSeq:WP_013526691.1", "SO:0001217", "UniParc:UPI0001F59E83", "UniRef:UniRef100_A0AB32XAT4", "UniRef:UniRef50_A0AB32XAT4", "UniRef:UniRef90_A0AB32XAT4" ], "nt": "TTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTGGCAGCTTGCTTAGAGTACTACATTTAA", "aa": "MIVSHDINEINTLADRIVIMNKGKVIFDKTKDEAVKEFKTLAACLEYYI", "aa_hexdigest": "7d87fa3978bb260db76778a16d5994e4", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59E83", "ncbi_nrp_id": "WP_013526691.1", "uniref100_id": "UniRef100_A0AB32XAT4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E83", "RefSeq:WP_013526691.1", "UniRef:UniRef100_A0AB32XAT4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAT4", "uniref90_id": "UniRef90_A0AB32XAT4" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAT4", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0AB32XAT4" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XAT4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAT4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_321", "locus": "GCA000186005_00282" }, { "type": "cds", "sequence": "contig_1", "start": 153516, "stop": 153650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGA", "aa": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHF", "aa_hexdigest": "524f818323dd96e7ee2b338dfc14b92b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5298.046899999999, "isoelectric_point": 10.118586921691893 }, "id": "EFPKPENHBP_322", "locus": "GCA000186005_00283" }, { "type": "cds", "sequence": "contig_1", "start": 153732, "stop": 153920, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTCGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTAGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGCTATTTGA", "aa": "MILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSAI", "aa_hexdigest": "9101cc85ce64dfdd67f680e611eaccea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6722.067, "isoelectric_point": 9.655059242248537 }, "id": "EFPKPENHBP_323", "locus": "GCA000186005_00284" }, { "type": "cds", "sequence": "contig_1", "start": 153939, "stop": 154058, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGA", "aa": "MVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIH", "aa_hexdigest": "89a559746d51c1689a36966f10a29b8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4509.1675, "isoelectric_point": 8.274984550476077 }, "id": "EFPKPENHBP_324", "locus": "GCA000186005_00285" }, { "type": "cds", "sequence": "contig_1", "start": 154293, "stop": 155102, "strand": "+", "frame": 3, "gene": null, "product": "ABC-2 type transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97", "UniRef:UniRef50_UPI000FF82EAB", "UniRef:UniRef90_C4XE97", "UniRef:UniRef90_UPI000FF82EAB" ], "nt": "ATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGAAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGA", "aa": "MAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENENDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILP", "aa_hexdigest": "7cffe5bea967eec53048794c8ac67e56", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82EAB", "query_cov": 0.9330855018587361, "subject_cov": 0.9061371841155235, "identity": 1.0, "score": 480.0, "evalue": 1.83e-170, "valid": true, "uniref50_id": "UniRef50_UPI000FF82EAB" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82EAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82EAB" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 153993, "stop": 155162, "observations": { "mutation": [ 154056, 154242, 155100, 155106, 155148 ], "stop": [ 154056, 154242, 155100, 155106, 155148 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE97", "query_cov": 1.0, "subject_cov": 0.4908759124087591, "identity": 1.0, "score": 515.0, "evalue": 5.12e-180, "gene_start": 1, "gene_end": 269, "reference_start": 260, "reference_end": 528, "reference_sequence": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHFWKAFVGPFFAFGYPIVFVIILGAVFNYEMILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSAIWTALVALMVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIHWAGYIYSAIVLTLVSIAVGLFIVSVSKSILTIQAIGSSLLIISMFLTGQVLPISQIIGQKVMWYLSYITPFKSPITQNTMAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENENDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILPWLWIVVLVGLSQKSFTWNTR" }, "paralog": false, "identity": 0.987146529562982, "score": 736.0, "evalue": 1.83e-269, "description": "Internal stop codon at 154056, 154242, 155100, 155106, 155148. Nonsense mutation around 154056, 154242, 155100, 155106, 155148.", "psc": { "uniref90_id": "UniRef90_C4XE97", "product": "ABC-2 type transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE97", "UniRef:UniRef50_C4XE97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97" ], "product": "ABC-2 type transporter domain-containing protein" } }, "id": "EFPKPENHBP_325", "locus": "GCA000186005_00286" }, { "type": "cds", "sequence": "contig_1", "start": 155264, "stop": 155434, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98", "UniRef:UniRef90_C4XE98" ], "nt": "TTGTTTAAAAAATATAAACTTGAAATTCAAATAGATTTAACTAATGATAAAGAAGATCTTGAAAAAAGAAACCATACAACAGCTAATAAATATATGGATTTAGTCTATAAAGTCAAAATTGCTGAAAAAAAGATTAAAGAAGGTTTTGCTGCATATTTAAAAATGCTATGA", "aa": "MFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKML", "aa_hexdigest": "c98de4fdb68c65f42dc564070b0ffdba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 155255, "stop": 155521, "observations": { "mutation": [ 155266, 155467 ], "stop": [ 155266, 155467 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE98", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.982, "score": 108.0, "evalue": 8.74e-30, "gene_start": 1, "gene_end": 56, "reference_start": 30, "reference_end": 85, "reference_sequence": "MYNNKKVFPFIKQEFNENWKLLTKEIVQNLFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKMLWNV" }, "paralog": false, "identity": 0.9659090909090909, "score": 169.0, "evalue": 2.08e-56, "description": "Internal stop codon at 155266, 155467. Nonsense mutation around 155266, 155467.", "psc": { "uniref90_id": "UniRef90_C4XE98", "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE98", "UniRef:UniRef50_C4XE98" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6740.905500000001, "isoelectric_point": 9.363597679138184 }, "id": "EFPKPENHBP_326", "locus": "GCA000186005_00287" }, { "type": "cds", "sequence": "contig_1", "start": 155534, "stop": 155650, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43", "UniRef:UniRef50_A0AB32XB43", "UniRef:UniRef90_A0AB32XB43" ], "nt": "ATGAATAGTGCATTAAGTACTTTAGCAACCTTACTTTTAACCAGTGCTTCATGCATTAAAGAAGACAGAATGTATGACAAGCCTGCTAAAGAAATTGATAAATATAAGCAGCTTTAA", "aa": "MNSALSTLATLLLTSASCIKEDRMYDKPAKEIDKYKQL", "aa_hexdigest": "ed80be0d1b049de55233c5bf81ab5965", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59E84", "uniref100_id": "UniRef100_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB43", "uniref90_id": "UniRef90_A0AB32XB43" }, "psc": { "uniref90_id": "UniRef90_A0AB32XB43", "uniref50_id": "UniRef50_A0AB32XB43" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XB43" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4290.974300000002, "isoelectric_point": 7.869091606140137 }, "id": "EFPKPENHBP_327", "locus": "GCA000186005_00288" }, { "type": "cds", "sequence": "contig_1", "start": 155771, "stop": 156478, "strand": "+", "frame": 2, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013526694.1", "SO:0001217", "UniParc:UPI0001F59E85", "UniRef:UniRef100_C4XE99", "UniRef:UniRef50_C5J6Z6", "UniRef:UniRef90_C4XE99" ], "nt": "ATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAACCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAA", "aa": "MPTPHISAQKGEIAKIVLMPGDPLRAEYMAKKFLKNPKLVSSVRNVLFYTGKYNGKDVTIGASGMGSASIGIYAYELFTVYEVETIVRVGSTGSYIKELNVKQPVLVKRAYADGTGFVELMVGNKKQENYPDLETYEKLKQTTKEMNVELKEVSCHSTDVFYGLRSLEDTIKITKCQTVDNECFALFATAQRCKKKAAALLSVSENIVTGASLSSDERLKEFSTMFLVALNALTK", "aa_hexdigest": "2052ad9099d0a63fd2ed41041f4d0e6f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59E85", "ncbi_nrp_id": "WP_013526694.1", "uniref100_id": "UniRef100_C4XE99", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E85", "RefSeq:WP_013526694.1", "UniRef:UniRef100_C4XE99" ] }, "ips": { "uniref100_id": "UniRef100_C4XE99", "uniref90_id": "UniRef90_C4XE99" }, "psc": { "uniref90_id": "UniRef90_C4XE99", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_C5J6Z6", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_C5J6Z6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6Z6" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "EFPKPENHBP_328", "locus": "GCA000186005_00289" }, { "type": "cds", "sequence": "contig_1", "start": 156489, "stop": 156998, "strand": "+", "frame": 3, "gene": "tpx", "product": "thiol peroxidase", "db_xrefs": [ "EC:1.11.1.-", "EC:1.11.1.24", "GO:0008379", "GO:0016684", "KEGG:K11065", "RefSeq:WP_013354398.1", "SO:0001217", "UniParc:UPI0001A8C0CB", "UniRef:UniRef100_C4XEA0", "UniRef:UniRef50_A8MM68", "UniRef:UniRef90_C4XEA0" ], "nt": "ATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGTCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGTGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAG", "aa": "MNKRQETTFFKNKVTLLGDKIEVGQKAPNFTALNNELKSVQLKDFKDKVILISSVPSLDTGVCQMQTVRFNKEVEQIKGLKVITISCDLPFAQSRFCTSFNTNNVVTLSDHKNLDFGLKYGCVIEELRLLNRAIFVIDKNGIIQYVEYVKENTDHPNYDKALKCVKNII", "aa_hexdigest": "2cd7c50fc38f1dd5528e8b82e42a6e94", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C0CB", "ncbi_nrp_id": "WP_013354398.1", "uniref100_id": "UniRef100_C4XEA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0CB", "RefSeq:WP_013354398.1", "UniRef:UniRef100_C4XEA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEA0", "uniref90_id": "UniRef90_C4XEA0" }, "psc": { "uniref90_id": "UniRef90_C4XEA0", "gene": "tpx", "product": "thiol peroxidase", "ec_ids": [ "1.11.1.-", "1.11.1.24" ], "uniref50_id": "UniRef50_A8MM68", "kegg_orthology_id": "K11065", "go_ids": [ "GO:0008379", "GO:0016684" ] }, "pscc": { "uniref50_id": "UniRef50_A8MM68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A8MM68" ], "product": "Thiol peroxidase" }, "genes": [ "tpx" ], "id": "EFPKPENHBP_329", "locus": "GCA000186005_00290" }, { "type": "cds", "sequence": "contig_1", "start": 157344, "stop": 157577, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1", "UniRef:UniRef90_C4XEA1" ], "nt": "ATGAAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGA", "aa": "MKKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKD", "aa_hexdigest": "28275387d2515573e29c00381c066fbf", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 157341, "stop": 157694, "observations": { "mutation": [ 157575, 157602, 157662 ], "stop": [ 157575, 157602, 157662 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA1", "query_cov": 1.0, "subject_cov": 0.6581196581196581, "identity": 1.0, "score": 147.0, "evalue": 2.01e-44, "gene_start": 1, "gene_end": 77, "reference_start": 2, "reference_end": 78, "reference_sequence": "MMKKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKDWAKEFLSKDWDKNKNTKKVIIFNLKRFLKWTTEELNKYQ" }, "paralog": false, "identity": 0.9658119658119658, "score": 221.0, "evalue": 5.22e-76, "description": "Internal stop codon at 157575, 157602, 157662. Nonsense mutation around 157575, 157602, 157662.", "psc": { "uniref90_id": "UniRef90_C4XEA1", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA1", "UniRef:UniRef50_C4XEA1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1" ], "product": "Lipoprotein" } }, "id": "EFPKPENHBP_330", "locus": "GCA000186005_00291" }, { "type": "cds", "sequence": "contig_1", "start": 157740, "stop": 157949, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTATCACTATTTTTGGATTTTTAACATCATTAATTAACTATGACAAATATGGTATTATAGGCTCATTTATGTATATTGCATTATTTGTTTCATTCTTAACATATTTTATGTTTTTAGGAATTATAGTTAATGAAGCATATTACAAAACTTATGTGTCTTCATACACTGTTTCTAAACGTAGTATATTTGGCAAAAAATTTTAA", "aa": "MIFITIFGFLTSLINYDKYGIIGSFMYIALFVSFLTYFMFLGIIVNEAYYKTYVSSYTVSKRSIFGKKF", "aa_hexdigest": "716b9c63099d5cc5a312984a991830d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8094.541599999997, "isoelectric_point": 9.477190971374515 }, "id": "EFPKPENHBP_331", "locus": "GCA000186005_00292" }, { "type": "cds", "sequence": "contig_1", "start": 158292, "stop": 158456, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTACTTTCGTTAATTCCTGGTTATCATACTTTATTTATTAATGAAGTTTTAACTCGAATTATTCCTGGTGTTACTTCTATTATTAAGATCCCTGACACCACAAAAAAAGGCGCTCAATTAACTTCTTTAATTATTCTTTGTTTGGCTTCTTTATGA", "aa": "MIVLLSLIPGYHTLFINEVLTRIIPGVTSIIKIPDTTKKGAQLTSLIILCLASL", "aa_hexdigest": "f908270ace9a5d457ffeac582491fca2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5847.109199999999, "isoelectric_point": 9.10185604095459 }, "id": "EFPKPENHBP_332", "locus": "GCA000186005_00293" }, { "type": "cds", "sequence": "contig_1", "start": 158839, "stop": 159243, "strand": "-", "frame": 1, "gene": "engA", "product": "GTP-binding protein EngA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "nt": "ATGGTTGAATTCGAAAAGAAAATGCGTAACAAATTTCATTTTGTACCTTGGGTTCCTATTTGTTTTGTTTCAGTTAAAACAAACTCAAGAATGGACAAATTAATTGACACAATAATTAAAGTTAAACAAAATCTTGAACGTGATGTTAAACCTTCAGTTCTTTCTAACTTAATCAGAGAAACACAAATGATTCAGCCAGCTCCTCCTTATAATGGTGGCCGTTTAAATATTTATTTTGCTCGTAAAACTCAAGATCCAGTTCCTACTTTTGTTTTCTTTGTTAACAATAAAAAATATGTCCACTTTAGCTATGAAAGATTTTTAGAAAAGCAAATTAGACAAGTTTTAGACTACACTGGTTGTCCAATTAATTTAGTTTTCAAAAACAAAAGTGGTTTAGAATAA", "aa": "MVEFEKKMRNKFHFVPWVPICFVSVKTNSRMDKLIDTIIKVKQNLERDVKPSVLSNLIRETQMIQPAPPYNGGRLNIYFARKTQDPVPTFVFFVNNKKYVHFSYERFLEKQIRQVLDYTGCPINLVFKNKSGLE", "aa_hexdigest": "368c090a2b6a3155c58b38da933c87c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0QDV4", "query_cov": 0.8432835820895522, "subject_cov": 0.9912280701754386, "identity": 0.6459999999999999, "score": 159.0, "evalue": 3.45e-48, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A3B0QDV4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0QDV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "product": "GTP-binding protein EngA" }, "genes": [ "engA" ], "id": "EFPKPENHBP_333", "locus": "GCA000186005_00294" }, { "type": "cds", "sequence": "contig_1", "start": 159271, "stop": 159741, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00350.29", "PFAM:PF02421.24" ], "nt": "GTGGATCATATTTTTCCTATTTCAGCTCAACATGGTAATGGTGTTGGTGATGTCTTAGACCAATGTCTTAAACATCTTAGACTTGATGATGAAAAGAGTGACAAACTATTCAAACTTTCAATTATTGGACGTCCTAATTCAGGTAAAAGTTCAATGTTAAATAGATTAGCAAATGAAAACCGTTCAATAGTTTCTGATATTGCGGGAACTACTAGAGATAGTGTTAAAAGTATTGTAAAAATCAAAGATGAAGTTTTTGAAATCATTGATACAGCTGGTATTACTAGAAAAAGTAAAATTGAAGATAGCGTTGACCATTATGCTTTAAAACGCGCTATGAGTTCACTTGACGAATCTAACTTATCTTTAGTTTTAATTGACTCAACTCAAGAATTATCACACTTCGATTCTAGAATTATAGGCTATGCTTTAGACAACAATAAACCAATAATTATTGTGGTTAATAAATGA", "aa": "MDHIFPISAQHGNGVGDVLDQCLKHLRLDDEKSDKLFKLSIIGRPNSGKSSMLNRLANENRSIVSDIAGTTRDSVKSIVKIKDEVFEIIDTAGITRKSKIEDSVDHYALKRAMSSLDESNLSLVLIDSTQELSHFDSRIIGYALDNNKPIIIVVNK", "aa_hexdigest": "0d3731e8754831daa3dbc867dd11e066", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00350.29", "name": "Dynamin_N", "length": 78, "aa_cov": 0.5, "hmm_cov": 0.9102564102564102, "evalue": 4.72805199486865e-09, "score": 32.799156188964844, "start": 78, "stop": 155 }, { "id": "PF02421.24", "name": "FeoB_N", "length": 118, "aa_cov": 0.7564102564102564, "hmm_cov": 0.9491525423728814, "evalue": 4.3330698105285473e-10, "score": 35.63899230957031, "start": 38, "stop": 155 } ], "seq_stats": { "molecular_weight": 17318.51749999999, "isoelectric_point": 6.193256187438965 }, "id": "EFPKPENHBP_334", "locus": "GCA000186005_00295" }, { "type": "cds", "sequence": "contig_1", "start": 159754, "stop": 159882, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATGGTGCTTCTGACATCACTAATGACGATTTAATGATTTACAGTATGCTTCAAAAATCTGGCAAACCTATTGTCGTAGTTGCAAACAAATTAGATAATATTTCACAGTTTAATTATTCATGA", "aa": "MVDGASDITNDDLMIYSMLQKSGKPIVVVANKLDNISQFNYS", "aa_hexdigest": "20814215acf8a8b0796e5cd1a8cbeede", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4636.2374, "isoelectric_point": 4.282727241516113 }, "id": "EFPKPENHBP_335", "locus": "GCA000186005_00296" }, { "type": "cds", "sequence": "contig_1", "start": 160003, "stop": 160146, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24" ], "nt": "ATGAGAAACAACGTTATAGCCATTATTGGAAAACCAAATGTTGGTAAAAGTACCCTATTTAATAGGCTAATTGGTAAAAGAAGTTCAATAATTTATGACCGTCCTGGAGTAACAAGGGACCGTCTTTATGAAACCTTTTCTTGA", "aa": "MRNNVIAIIGKPNVGKSTLFNRLIGKRSSIIYDRPGVTRDRLYETFS", "aa_hexdigest": "34ef83a718c1b72f31f2c9c8bf5c3e78", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3S4P1B8", "query_cov": 0.8297872340425532, "subject_cov": 0.8297872340425532, "identity": 0.615, "score": 56.6, "evalue": 8.25e-10, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A4Z0KSI3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 37, "aa_cov": 0.7872340425531915, "hmm_cov": 0.972972972972973, "evalue": 6.38379586075356e-09, "score": 31.843690872192383, "start": 5, "stop": 41 } ], "seq_stats": { "molecular_weight": 5368.181400000001, "isoelectric_point": 10.956482505798338 }, "id": "EFPKPENHBP_336", "locus": "GCA000186005_00297" }, { "type": "cds", "sequence": "contig_1", "start": 160150, "stop": 160458, "strand": "-", "frame": 1, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0015949", "SO:0001217", "UniRef:UniRef50_A5IZG6", "UniRef:UniRef90_C4XEA4" ], "nt": "ATGGATGGAAGAGATACTACATTCAAAATTATGCCTTATGCAGAATTAAAAATCTTTTTAACAGCAACACCAGAAGAAAGAGCAATGAGAAGATTAAACCAAAACATTGCTTTAGGATTTAATATGGACTACAACAAAGTTCTTGAAGAAGTTAAATTAAGAGACTATCAAGACATGAATCGTGAAGTAGACCCTCTTCATAAAGTTAAAGAAGCAAAAGAAATAGACTGTACAAATATGGGTTTTGAAGCAGTTGTTGTTGAAATTATTGAACTAGTTAAAAAAACAATAGAAAAACTTAATAAATAA", "aa": "MDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK", "aa_hexdigest": "d55537de57d95860022538db926cbe00", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 160150, "stop": 160758, "observations": { "mutation": [ 160674 ], "stop": [ 160674 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA4", "query_cov": 1.0, "subject_cov": 0.4473684210526316, "identity": 1.0, "score": 202.0, "evalue": 3.19e-64, "gene_start": 1, "gene_end": 102, "reference_start": 127, "reference_end": 228, "reference_sequence": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVDWNNEEEINKTLEKDIITLDERENIFLREKNVSVEIREDSISYGSSTIATYKKVRQYVVNLIQNITKKGKGYIMDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK" }, "paralog": false, "identity": 0.995049504950495, "score": 391.0, "evalue": 3.88e-141, "description": "Internal stop codon at 160674. Nonsense mutation around 160674.", "psc": { "uniref90_id": "UniRef90_C4XEA4", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_A5IZG6", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0015949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA4", "EC:2.7.4.25", "UniRef:UniRef50_A5IZG6", "GO:0004127", "GO:0005524", "GO:0015949" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZG6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZG6" ], "product": "Cytidylate kinase" } }, "id": "EFPKPENHBP_337", "locus": "GCA000186005_00298" }, { "type": "cds", "sequence": "contig_1", "start": 160672, "stop": 160836, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13207.12", "PFAM:PF02224.24" ], "nt": "ATGACAAAAATTACAAAGATTAATATTGCAATTGATGGACCTTGCGGTGCAGGTAAATCAACTGTTTCACAAGAAGTAGCAAGAAGGCTTGGATACACATTTATTAATAGTGGAAGTGTGTATAGAGCATTAGCTTTAAATGCAAAATTATTAGGTGTTGATTGA", "aa": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVD", "aa_hexdigest": "2701fd7a256c8bd6ee28a59ae86263a8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13207.12", "name": "AAA_17", "length": 38, "aa_cov": 0.7037037037037037, "hmm_cov": 1.0, "evalue": 9.535547460068491e-09, "score": 31.983428955078125, "start": 13, "stop": 50 }, { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 40, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 2.3621940863627957e-16, "score": 56.42131042480469, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5683.5617, "isoelectric_point": 9.743251991271972 }, "id": "EFPKPENHBP_338", "locus": "GCA000186005_00299" }, { "type": "cds", "sequence": "contig_1", "start": 160925, "stop": 161155, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTAACTCAAATTATAAACTTCAAAGTGTTGATTTATATACCTTTGTAGATAGATTAGAAAAATTAATCAAAAATTCCCAATTGCCCTATGAAAAAGACAAAAAAGGAACTGAAAAAGCTGAAGAAAAATATAAAAATTTTAAAAGAAATTTTCTTGAACCTATTGACAATATGAAGCAAATTATAAATGATACAGTGCTATTAATTAATGAATACAAATAA", "aa": "MIVFNSNYKLQSVDLYTFVDRLEKLIKNSQLPYEKDKKGTEKAEEKYKNFKRNFLEPIDNMKQIINDTVLLINEYK", "aa_hexdigest": "6ac33935e39f8d85c25834f75ffd5f4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.484499999995, "isoelectric_point": 8.802593803405763 }, "id": "EFPKPENHBP_339", "locus": "GCA000186005_00300" }, { "type": "cds", "sequence": "contig_1", "start": 161564, "stop": 161947, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAATATGAAAAAAATAATTTTGTTAGCAATCTAAAACAAAATGTCTCTATTTATACAAATAACTTTAATAAATTAGTTAAATTTATGGACAAGGATAACCACAAATTGTCATATCAATCATTTTATTATAAGGAATATGAAAAAACTAAAGAAATAACTGATTTCACATTTGAAAACTTTTTATTAAAAAACTCAAAAGCTTTTGAAGAAAAAGCATATAAATTTATGGTTAATAATGTTGATGAAAGTACTAAATTCAAAAAATATTTTGAAGACTTTACATCTACTCCTAGTGATAATACTAATTTTAATTTTTATAAAAATGTAACCTATTTGAAGAAAATAAATGATGAAATAAATTCAATTTTAAATGACTGA", "aa": "MGEYEKNNFVSNLKQNVSIYTNNFNKLVKFMDKDNHKLSYQSFYYKEYEKTKEITDFTFENFLLKNSKAFEEKAYKFMVNNVDESTKFKKYFEDFTSTPSDNTNFNFYKNVTYLKKINDEINSILND", "aa_hexdigest": "d57bb2f903f709e1ed7fd177013c66c6", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15383.972799999987, "isoelectric_point": 6.605282020568848 }, "id": "EFPKPENHBP_340", "locus": "GCA000186005_00301" }, { "type": "cds", "sequence": "contig_1", "start": 162025, "stop": 162408, "strand": "-", "frame": 1, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.22", "KEGG:K06178", "SO:0001217", "UniRef:UniRef50_A0A454AP59", "UniRef:UniRef90_C4XEA6" ], "nt": "ATGATTAATAAACTAACACATCCTAAATACGAAATTTTACGTACATATCGTGTTAGAATTGATTCTCCTTTAAGCTTAAAAGAATTCAAATTAATTAACGCTGGTTTGATTGTTAATGGTAAGTTTTCAAAACAAATAGTTGACCAAGTTGATACTAAAAGTTATTTAGTAACTCTTCATGTCGGCTCATACCATCACATCAAAGAATTATTCAAAGCATTAAATCATGAAGTTATTAATCTTAAAAGAGTGTCATTTGCTAACTTAACAGTTGAAAAAATGCCTGAAGGCTCATATCGAACTTTAACTCTTAAAGAAATAAAAGATCTTAAAGTTTTAATTTCATTGCAAGAAAAGAAATTATGTCAAAAAGAAGAAAATTAA", "aa": "MINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN", "aa_hexdigest": "55c819be69260b4bad3d8a552b4cfb79", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 162025, "stop": 162708, "observations": { "mutation": [ 162414 ], "stop": [ 162414 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA6", "query_cov": 1.0, "subject_cov": 0.4721189591078067, "identity": 1.0, "score": 248.0, "evalue": 3.89e-81, "gene_start": 1, "gene_end": 127, "reference_start": 143, "reference_end": 269, "reference_sequence": "MYTRTTKNLWQMNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTNDWEMINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN" }, "paralog": false, "identity": 0.9955947136563876, "score": 441.0, "evalue": 1.02e-159, "description": "Internal stop codon at 162414. Nonsense mutation around 162414.", "psc": { "uniref90_id": "UniRef90_C4XEA6", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.22" ], "uniref50_id": "UniRef50_A0A454AP59", "kegg_orthology_id": "K06178", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA6", "EC:5.4.99.22", "UniRef:UniRef50_A0A454AP59", "KEGG:K06178" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454AP59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454AP59" ], "product": "Pseudouridine synthase" } }, "id": "EFPKPENHBP_341", "locus": "GCA000186005_00302" }, { "type": "cds", "sequence": "contig_1", "start": 162412, "stop": 162801, "strand": "-", "frame": 1, "gene": null, "product": "pseudouridine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "nt": "ATGAATGAAATAAATTTTTTGGTAGAAAGGTTTGCAATGAAAGAATTGATTAGGGTTCAAAAACTAATCGCACAAACAGGATATTGTTCAAGAAGAAAAGCTGAAGAATATATTGAAAAAAAACTAGTTAAAGTTAATGGAAAAATTGTTCAATTAGGTGACAAAGCAAGTGAAAACGATGAGATAAAAATTAACAATCAACTAATCTCATTACAAAAACCAAATTTTGTATATTTCCTATTAAATAAGCCTAAAAAGACAATTTCAACAGTTAAAGATCCTAAGGGCAGAAAAACAGTTGTTGATTTAATTAATTACTCAGAAAGAATTGCTCCTGTAGGTCGTTTAGACTATGATACAACTGGCGCTTTGTTATTAACAAATGATTGA", "aa": "MNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTND", "aa_hexdigest": "67f54b5998efcf146e26742f1cbe475a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FC9400C", "query_cov": 0.9147286821705426, "subject_cov": 0.9147286821705426, "identity": 0.508, "score": 112.0, "evalue": 1.1e-29, "valid": false, "product": "pseudouridine synthase", "uniref50_id": "UniRef50_UPI002FC9400C" }, "pscc": { "uniref50_id": "UniRef50_UPI002FC9400C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "product": "pseudouridine synthase" }, "genes": [], "id": "EFPKPENHBP_342", "locus": "GCA000186005_00303" }, { "type": "cds", "sequence": "contig_1", "start": 163074, "stop": 163379, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08668.18" ], "nt": "ATGGTACCTGAAAAAGTTAACCAATATATAGGAAGTCACTTTTTATATGCTTTTGAAATAGTTCACGCTTTGCTTAGTGCAAAAAGAGCAAAACATTCAGTTTTTGCAGCAACTTTTGCTGCTGAGTTAGCTAAAACAATAAAATATGATGCAAAAGTGGCTTACTATGCTGGACTATTTCATGATATTTGTAAAGAATTAAGTGAAAATGAAAGTAGAAAATTTATTGCTCAATATGATAAAAAAGCTAACAACAAATCTATTTATCCAAATCATGTTTTACATCAAGTTTGTGGAGCTTTATGA", "aa": "MVPEKVNQYIGSHFLYAFEIVHALLSAKRAKHSVFAATFAAELAKTIKYDAKVAYYAGLFHDICKELSENESRKFIAQYDKKANNKSIYPNHVLHQVCGAL", "aa_hexdigest": "d91cccf887183e7bb44afaeda972a71e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08668.18", "name": "HDOD", "length": 62, "aa_cov": 0.6039603960396039, "hmm_cov": 1.0, "evalue": 1.3945145732116456e-06, "score": 24.255308151245117, "start": 29, "stop": 89 } ], "seq_stats": { "molecular_weight": 11435.049399999989, "isoelectric_point": 9.077486991882324 }, "id": "EFPKPENHBP_343", "locus": "GCA000186005_00304" }, { "type": "cds", "sequence": "contig_1", "start": 163536, "stop": 163883, "strand": "-", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAAATAGGATTATTTGGTGGATCATTCAATCCAATCCATAGTGGACATATCAAAATTGCTGAATATGCCTATAAAACACTTAAATTAGACAAAATGTTTTTTATACCAACAGCGATAAGCCCATTTAAAAAGAATAATAAAGTAGCCCCTAATAAAGACAGAGTTAATATGATTAATATAGCTATTGAAAATCTAGAAGGCAATTATGCTGTGCATGATTTTGAAATCAAAAAAGGTGGCGTTAGTTATACTTTTGAAACAATTAGATATTTCAAACAACAATATCCAAATGATGAATTATATTTCTTAATAGGCAGCGATTTATTGCCAAAATTAAATAAATGA", "aa": "MKIGLFGGSFNPIHSGHIKIAEYAYKTLKLDKMFFIPTAISPFKKNNKVAPNKDRVNMINIAIENLEGNYAVHDFEIKKGGVSYTFETIRYFKQQYPNDELYFLIGSDLLPKLNK", "aa_hexdigest": "b1dec7b34b8b3b87832ee5bfd24113d9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.0, "subject_cov": 1.0267857142857142, "identity": 0.5479999999999999, "score": 123.0, "evalue": 3.13e-34, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "EFPKPENHBP_344", "locus": "GCA000186005_00305" }, { "type": "cds", "sequence": "contig_1", "start": 164530, "stop": 164856, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11798.14" ], "nt": "GTGTTTTCAATTGATGAATGTTGGCTTGATGTAACTGATATTATCAAAAATAGAGATCCAAAAGATTTGGCTAAAAGTATCCAAAGTGACATATTAAATACTTTTGACATTCCTGCAACAATTGGAATTTCATACAACAAATTTTTAGCAAAATTATCAACTAATTTAGGTAAACCATTTGGAATTCTAGAAACAAAAGAGGAAGACATAGAAAAAAGGATTTGGCCTTTACCAATTATTGAATTTTTCGGTATTGGTGCTCCAACAGCTGAGAGATTAATTAGAATGCATATTGATACTATTGGCAAATTAGCAAAATGTAATTGA", "aa": "MFSIDECWLDVTDIIKNRDPKDLAKSIQSDILNTFDIPATIGISYNKFLAKLSTNLGKPFGILETKEEDIEKRIWPLPIIEFFGIGAPTAERLIRMHIDTIGKLAKCN", "aa_hexdigest": "6a332b7d0156303f109f8fd90ed536aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11798.14", "name": "IMS_HHH", "length": 31, "aa_cov": 0.28703703703703703, "hmm_cov": 1.0, "evalue": 8.630856400700751e-07, "score": 25.29746437072754, "start": 68, "stop": 98 } ], "seq_stats": { "molecular_weight": 12236.127199999983, "isoelectric_point": 5.35516529083252 }, "id": "EFPKPENHBP_345", "locus": "GCA000186005_00306" }, { "type": "cds", "sequence": "contig_1", "start": 164983, "stop": 165159, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAAAACAATTTTTCACATGGATTTTGATTCATATTTTGTAAGTGCTCATCGCTCAATTAATCCTGAATTAAAAGGAAAATCAGTAGCTATTGGTAGAAGATTAAAAAGATCAATAGTTTCTTCAATTAGTTATGAGTTAAAAAGAAAAGGCGCTAAAGTTGGCTGA", "aa": "MENKKTIFHMDFDSYFVSAHRSINPELKGKSVAIGRRLKRSIVSSISYELKRKGAKVG", "aa_hexdigest": "607e38b44feedb1fde82dc651c692c95", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6612.6438, "isoelectric_point": 10.345902442932129 }, "id": "EFPKPENHBP_346", "locus": "GCA000186005_00307" }, { "type": "cds", "sequence": "contig_1", "start": 165149, "stop": 165577, "strand": "-", "frame": 3, "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "db_xrefs": [ "GO:0005198", "GO:0016226", "RefSeq:WP_013526700.1", "SO:0001217", "UniParc:UPI0001F59E8A", "UniRef:UniRef100_A0AB32XAS2", "UniRef:UniRef50_C4XEA9", "UniRef:UniRef90_C4XEA9" ], "nt": "TTGTCTTATAATAAAGATCAAAAACGTCAAATAATAATGGAACATTATTTGAAACCTAGACACAAAAGTTCAAGTTTAAAAAACAAAATTATTAAGCACGGAGAAAGCTGTGCTGATTATTTAGAATGTGAACTAAATATAAAAAATAATCAAATTGAAGACATTAAATTTGATGGTCAAGGTTGTGCATTTTTTATAGCTTCAACTGATTTATTAATCGACTTATTAAAAGGTAAAAAAATTGACGAAAGCATCTGTTTGATAGAACTTTACGAAAAATTCATACTAGATGAAAAATTAAATGAAAATGATATTGAAAAGCTAGAAACTTTAGCTATTTTTGATAATGTTAAAACACAATTTAATCGCGTTAATTGTGCTTTAATGTTAGCTAGAGCAATAAAGGAAAAATTGCAAGATGGAAAATAA", "aa": "MSYNKDQKRQIIMEHYLKPRHKSSSLKNKIIKHGESCADYLECELNIKNNQIEDIKFDGQGCAFFIASTDLLIDLLKGKKIDESICLIELYEKFILDEKLNENDIEKLETLAIFDNVKTQFNRVNCALMLARAIKEKLQDGK", "aa_hexdigest": "2e35a2eeba09b0c09c824cfe5cee97ee", "start_type": "TTG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59E8A", "ncbi_nrp_id": "WP_013526700.1", "uniref100_id": "UniRef100_A0AB32XAS2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E8A", "RefSeq:WP_013526700.1", "UniRef:UniRef100_A0AB32XAS2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAS2", "uniref90_id": "UniRef90_C4XEA9" }, "psc": { "uniref90_id": "UniRef90_C4XEA9", "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "uniref50_id": "UniRef50_C4XEA9", "go_ids": [ "GO:0005198", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA9" ], "product": "NIF system FeS cluster assembly NifU N-terminal domain-containing protein" }, "genes": [ "sufU" ], "id": "EFPKPENHBP_347", "locus": "GCA000186005_00308" }, { "type": "cds", "sequence": "contig_1", "start": 165567, "stop": 165989, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGTCAACAGCAGCTGATCGCTTTGAAGCTGGAACACCTAATTTAGCAGCAATTAATATGTTTAGTGTTTCAATAAACTTTTTTAATCAAATTGGTTATTGTGAAACTCAAAAAATTTTAGACGAATTAAGCTTTTATGCTTATCAAGAATTAAGCAAAATTGAAGGCATAAAACTATATAGCAATCCTAAAAATCACATTATAATATTCAATATAAAAAACTATAATAGTCATGATGTTTCACATTATTTAGGAACAAAAAATATTTATGTAAGAGCAGGTGTATTTTGTGATCATTATCTTAAAAAAATCACCAAAGAAAAGTCTTTTGTAAGAGTTTCTTTGGGTATTTATAACACCAAAAAAGATATTGATAAATTAGTTAAAGCATTAAAAGAAGGAGGTGATTTCCTTGTCTTATAA", "aa": "MSTAADRFEAGTPNLAAINMFSVSINFFNQIGYCETQKILDELSFYAYQELSKIEGIKLYSNPKNHIIIFNIKNYNSHDVSHYLGTKNIYVRAGVFCDHYLKKITKEKSFVRVSLGIYNTKKDIDKLVKALKEGGDFLVL", "aa_hexdigest": "74f1b85d2cf308b30f7136d608f54491", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 128, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 1.1176124792188423e-29, "score": 99.8290023803711, "start": 4, "stop": 127 } ], "seq_stats": { "molecular_weight": 16000.246899999984, "isoelectric_point": 8.969631385803225 }, "id": "EFPKPENHBP_348", "locus": "GCA000186005_00309" }, { "type": "cds", "sequence": "contig_1", "start": 166362, "stop": 166727, "strand": "-", "frame": 2, "gene": null, "product": "aminotransferase class V-fold PLP-dependent enzyme", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "nt": "ATGAAAACATTAGACAAATTAATTCAAGAACAATTCCCTTTAGCAAAAAAGATAACTTATTTGGATAGTAGTGCATTAGTTTTAAAACCAAAAATGGCTATTGATGCAGTTAACAATTTTTATTTAAATGAGTCAATTAGTACCCGTACTATTAACACTAAATTAGGAATTGAAAGCGCGCAAAAAATAAACAACTTACGTAAAAAAGTTGCTACTTTGTTGTCTTGTCAAGAACAAGAAGTTATATTTACTTCAGGAGCAACTGATTCACTAAACAAGTTTGCTTTAATGATCAAAAATCAAATTAAAAAAGGGGATATTATCCTTTTAGATGTTTATAATCATTCTTCAAATATGGCGCCTTGA", "aa": "MKTLDKLIQEQFPLAKKITYLDSSALVLKPKMAIDAVNNFYLNESISTRTINTKLGIESAQKINNLRKKVATLLSCQEQEVIFTSGATDSLNKFALMIKNQIKKGDIILLDVYNHSSNMAP", "aa_hexdigest": "2f416e543c03322904c436cf14dba10b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028BE3002", "query_cov": 0.9421487603305785, "subject_cov": 0.8837209302325582, "identity": 0.526, "score": 117.0, "evalue": 1.75e-31, "valid": false, "product": "aminotransferase class V-fold PLP-dependent enzyme", "uniref50_id": "UniRef50_UPI0024C98C43" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C98C43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "product": "aminotransferase class V-fold PLP-dependent enzyme" }, "genes": [], "id": "EFPKPENHBP_349", "locus": "GCA000186005_00310" }, { "type": "cds", "sequence": "contig_1", "start": 166958, "stop": 167413, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13692.12" ], "nt": "TTGGTTTGGGAAAAAAATATTAAAAAAGTTCTATTAACTTATAAAAAACTTGTTGAAGAAAATTCTAATTACCATTTAACAATGGTTGGTGGTGATATCAATTACAAGGAAATTGTTAAATATGCCTATAAAATAGGTTTAAAAGATAAAATATTGTTTACTGGCCCTATAGAAAATCAAGAAGAATTAAAAGGCATTTATTTATCTCATGATCTTTTTTTCTATCCAAGTTTATATGAAACTTATGGACTTGTTGTTAGAGAATCTGCAGCTTTAAAGTTGCCTCTCTTAGTTGTTGAAAACACAGCATGTGCCGAAAATGTGATAGATGGACAAAATAGTTTTATTTGCCAAGACGATGTTGACAATATGTACGAAAAAATTAAACAAATTTTTAAAGATAAAAATAATCTAATTAATGTGAAAAATAATATGAACAATTTATTTAATTCATGA", "aa": "MVWEKNIKKVLLTYKKLVEENSNYHLTMVGGDINYKEIVKYAYKIGLKDKILFTGPIENQEELKGIYLSHDLFFYPSLYETYGLVVRESAALKLPLLVVENTACAENVIDGQNSFICQDDVDNMYEKIKQIFKDKNNLINVKNNMNNLFNS", "aa_hexdigest": "0b68726ac7b29655a1b4aa37cd2c1a18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13692.12", "name": "Glyco_trans_1_4", "length": 135, "aa_cov": 0.8675496688741722, "hmm_cov": 0.9555555555555556, "evalue": 1.4219471502069472e-19, "score": 67.07129669189453, "start": 4, "stop": 134 } ], "seq_stats": { "molecular_weight": 17527.011300000006, "isoelectric_point": 5.728370094299317 }, "id": "EFPKPENHBP_350", "locus": "GCA000186005_00311" }, { "type": "cds", "sequence": "contig_1", "start": 167456, "stop": 167587, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAAAAGTTGCGCAAAAAGCTTTAATTGAATATGGATATAAAAAAGAAAGTGAAATTGTACAATTAGGGACAAATTTCTTTTATCCAGAAAATGCAGATGAATTAAAAAATAAAGTTATAAAAAAATGA", "aa": "MSKVAQKALIEYGYKKESEIVQLGTNFFYPENADELKNKVIKK", "aa_hexdigest": "e5d117b08f961fae864ee84be65e4df8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4993.729100000001, "isoelectric_point": 9.004702186584474 }, "id": "EFPKPENHBP_351", "locus": "GCA000186005_00312" }, { "type": "cds", "sequence": "contig_1", "start": 167813, "stop": 168073, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAGTGAAATTAGAGTTATTTTAATTAATGATAGTTTTACTCCTGTAATAGATGGAGTTGTCAGGGTAATGCAAAATTATGCTTCTAATTTTATTAAAAAAAATGTTAAAGTTCTGGTTTTGGCTCCAGAATACAAAAAAATGAAATTAGATGAAGATAAAGGTCTTAATTATTCTGTCATTAGAATACCTAGACAATTAGCTAAAATTGGTGGTTATGAAGTAATGAACACTATTTTGAGTAGAAAAATTTGA", "aa": "MTNSEIRVILINDSFTPVIDGVVRVMQNYASNFIKKNVKVLVLAPEYKKMKLDEDKGLNYSVIRIPRQLAKIGGYEVMNTILSRKI", "aa_hexdigest": "91b8d0591ea5591d0999f147bee4d1b0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9842.572999999995, "isoelectric_point": 9.791796684265137 }, "id": "EFPKPENHBP_352", "locus": "GCA000186005_00313" }, { "type": "cds", "sequence": "contig_1", "start": 168159, "stop": 168380, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAAAACCAAATTATCAAAAAGCTCTTGAGACTTTGTATGAACCAAATTCAAATATTCATCATGTAATTAATTGGCCTTTTAAAAAGGCTACAAAAATTAACATATATGAAAATATCAATTTTTTTGATACTATTGATGTTAAATTTGAAGATATAAAAATTCGGGTGCCAAAAAATTATGAAACAATATTTTTACAAAACTATGGTCCAAATTGA", "aa": "MFKKPNYQKALETLYEPNSNIHHVINWPFKKATKINIYENINFFDTIDVKFEDIKIRVPKNYETIFLQNYGPN", "aa_hexdigest": "0595a260e0fd2fc1e42b5d7c9cb9dc8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8838.021799999995, "isoelectric_point": 8.89278507232666 }, "id": "EFPKPENHBP_353", "locus": "GCA000186005_00314" }, { "type": "cds", "sequence": "contig_1", "start": 168420, "stop": 168590, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCCCTAAATTTTCAAAAGATAAAGATTCTCAAAAAGATGTAACTTTTTTAGATCTTTTTATCGATATACCAACAACTAAAAAAAGAATAAAAAAATATTTATCTCTTAAAAATAAATTGGGTTATTTGAACGCTTATTCTCAAAGAAAGTTTTTTGATATTCAATGA", "aa": "MFPKFSKDKDSQKDVTFLDLFIDIPTTKKRIKKYLSLKNKLGYLNAYSQRKFFDIQ", "aa_hexdigest": "4bef104fc0092c4b94310fbf3e1ec783", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6751.8451000000005, "isoelectric_point": 9.8733491897583 }, "id": "EFPKPENHBP_354", "locus": "GCA000186005_00315" }, { "type": "cds", "sequence": "contig_1", "start": 168693, "stop": 168827, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04991.19" ], "nt": "ATGAATGAAAAACAAACTAAAGTTTATGAACTGTTGAAAGAGTTTATTGAAATTTGTAATAAACACAATTTAGATTATTGTGCTATGTATGGAACAGCATTAGGAGCAATAAGACACAATGGATTTATACCTTGA", "aa": "MNEKQTKVYELLKEFIEICNKHNLDYCAMYGTALGAIRHNGFIP", "aa_hexdigest": "d5025df6f2ee5b016ad3728226234bea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04991.19", "name": "LicD", "length": 26, "aa_cov": 0.5909090909090909, "hmm_cov": 1.0, "evalue": 3.663060075358907e-09, "score": 33.315032958984375, "start": 19, "stop": 44 } ], "seq_stats": { "molecular_weight": 5117.8992, "isoelectric_point": 6.6983842849731445 }, "id": "EFPKPENHBP_355", "locus": "GCA000186005_00316" }, { "type": "cds", "sequence": "contig_1", "start": 168904, "stop": 169893, "strand": "-", "frame": 1, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "EC:1.1.1.399", "KEGG:K25634", "RefSeq:WP_013526703.1", "SO:0001217", "UniParc:UPI0001F59E8C", "UniRef:UniRef100_A0AB32XB50", "UniRef:UniRef50_A0A3P8K9L4", "UniRef:UniRef90_C4XEB3" ], "nt": "ATGAAAATTATTACTTTTAAAGTTAGAAATGTTGAAAAACCAATATTTGAAGAAATTAACAAAAAATTTGGTTATAAACTTGAAATTCATAGTGAAAGTTTAACTATGGAAAATGTCAATTTAACAAAAGGTTTTGACGCTGTTATTGTTAGAGTTGATGGTTCAATAAATAAAGAAATGTTAGATGCATTAAAAGCAAATGGCATTAAATATCTTTTAACAAGAACTGTCGGAACAGACCATATTGATTTAGAATATGCTCATAAATTAGGCTTTAAAATGGCTAGAGTTCCTTCATATTCACCAACAGCAATTGCTGAATTAGCTTTTGCTCATGCTTTAATGTTATCAAGACAAACACTTCATTTTGGACATAAAGGTTTTAACAAAGACTTTACTATTGATCCTGAAGGTTTTGCTACTGAATTAAAAAATAAAACAGTTGGAATAGTTGGGGTTGGAAAAATAGGACTTGCAGCTGCTAAAATGTTTAAAGTTATGACAAACAATGTTTTAGGCTTCGATTTATATCCTTCAGAAGAAGGCAAAAAAGTTGTTAAATATACAAAAACTCTAGAAGAATTAGTTAAAAAATCAGATATCATTTCTTTCCATTGCCCCTATATTAAAGGTAAAAACGACAAAATGATTAATGATGCATTAATTAAGCAAATGAAAAAAGGTGTAATTATTGTTAACACAGCTAGAGGCCAAATTCAAGATGAAGAAGCATTATTAAAAGGTCTAAAAAGTGGCAAAATTCAAGCTATTGCTACAGATGTTTTGAACAATGAAAAATCAATTTTCTTTAAAAAGCATGCAGAAATAGAAAACAAAACATTTCGCCAATTATTAGAATTCTATCCTCGTTTTGTTTTAACACCTCACATTGGTTCTTATACTGATGAAGCTGTTGAAAACATGGTTGAATACACTTATGACAATCTAAAAGAATATATCGAAACTGATCAATGCAAAAATGCAATTTAA", "aa": "MKIITFKVRNVEKPIFEEINKKFGYKLEIHSESLTMENVNLTKGFDAVIVRVDGSINKEMLDALKANGIKYLLTRTVGTDHIDLEYAHKLGFKMARVPSYSPTAIAELAFAHALMLSRQTLHFGHKGFNKDFTIDPEGFATELKNKTVGIVGVGKIGLAAAKMFKVMTNNVLGFDLYPSEEGKKVVKYTKTLEELVKKSDIISFHCPYIKGKNDKMINDALIKQMKKGVIIVNTARGQIQDEEALLKGLKSGKIQAIATDVLNNEKSIFFKKHAEIENKTFRQLLEFYPRFVLTPHIGSYTDEAVENMVEYTYDNLKEYIETDQCKNAI", "aa_hexdigest": "b97e1c810d70dc9fd674fd8fe8c9f625", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59E8C", "ncbi_nrp_id": "WP_013526703.1", "uniref100_id": "UniRef100_A0AB32XB50", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E8C", "RefSeq:WP_013526703.1", "UniRef:UniRef100_A0AB32XB50" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB50", "uniref90_id": "UniRef90_C4XEB3" }, "psc": { "uniref90_id": "UniRef90_C4XEB3", "product": "D-lactate dehydrogenase", "ec_ids": [ "1.1.1.399" ], "uniref50_id": "UniRef50_A0A3P8K9L4", "kegg_orthology_id": "K25634" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K9L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K9L4" ], "product": "D-lactate dehydrogenase" }, "genes": [], "id": "EFPKPENHBP_356", "locus": "GCA000186005_00317" }, { "type": "cds", "sequence": "contig_1", "start": 169932, "stop": 170066, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTTTTGCAGCTACACCACCTGCTACAGTTGCTGTTGGTTTTGCAATTAGTGAAAATAAATGTGCTAGATTAGCAAGCTCTGCTTCAGCATTTAGTACATTACTTGCAGTTATCTTTTTACCTATTTGA", "aa": "MVIFAATPPATVAVGFAISENKCARLASSASAFSTLLAVIFLPI", "aa_hexdigest": "c24c58320307d048a84b3244beda3903", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4468.282899999997, "isoelectric_point": 7.975980186462403 }, "id": "EFPKPENHBP_357", "locus": "GCA000186005_00318" }, { "type": "cds", "sequence": "contig_1", "start": 170313, "stop": 170513, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAATTAATACCAGGTGCAGGAGCAACATATAAAGATTTTAGTCAAACTATTAAAGTTAATGGAAGTGATGTAGTTATTAAATATTCAGCTACTTTTGGTGAAAACTTTAAAACAGTTTTAACACCAGACAAAAAAGTATTTTATATGTTAAATGCAAATAATGAATATATTAAAACCACTAAACCAGTTGGTTGA", "aa": "MNQLIPGAGATYKDFSQTIKVNGSDVVIKYSATFGENFKTVLTPDKKVFYMLNANNEYIKTTKPVG", "aa_hexdigest": "fa4802d779d6d150b0055bd852f23f15", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7308.300000000002, "isoelectric_point": 9.302352714538571 }, "id": "EFPKPENHBP_358", "locus": "GCA000186005_00319" }, { "type": "cds", "sequence": "contig_1", "start": 170514, "stop": 170621, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATGAAAGGTTTAAAATTCAACAAAGAAAATATTAAAGCTTCAGCAAAATCAATGTTTTTAAACCCAATTATTATTGCAACTTTTGCTGGATTAATTCTTTGA", "aa": "MQMKGLKFNKENIKASAKSMFLNPIIIATFAGLIL", "aa_hexdigest": "78cfbf956a516b43818a46747f703483", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3882.7434999999987, "isoelectric_point": 10.176350593566895 }, "id": "EFPKPENHBP_359", "locus": "GCA000186005_00320" }, { "type": "cds", "sequence": "contig_1", "start": 170757, "stop": 170870, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCATAAAACACCAGAATCAGTGGAAAATGCTCAAGAAAACACTATTATTGGTGAAATGAGTGAAACAAAAACAGTTGATGAATCTCGTCATCGCGCAATGGTTGTTTGA", "aa": "MPHKTPESVENAQENTIIGEMSETKTVDESRHRAMVV", "aa_hexdigest": "10755b970deafaacb484efd14149e65c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4182.627900000001, "isoelectric_point": 5.086316871643065 }, "id": "EFPKPENHBP_360", "locus": "GCA000186005_00321" }, { "type": "cds", "sequence": "contig_1", "start": 170886, "stop": 171017, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTGCTTTACCAGCTTTAGCACTCAAAGGCTTTATGAGCGATATTAGTCTTGGACTTCTTCAAGAACAAGGTATTATCTTAGGAATTGCATTTGGATTCTATATCTTGCTTTCATTAGTGGCTTGA", "aa": "MKVALPALALKGFMSDISLGLLQEQGIILGIAFGFYILLSLVA", "aa_hexdigest": "68ad2e2e4a7845b60a4de1f7e9b59846", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4536.525399999999, "isoelectric_point": 5.8228364944458 }, "id": "EFPKPENHBP_361", "locus": "GCA000186005_00322" }, { "type": "cds", "sequence": "contig_1", "start": 171573, "stop": 171824, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGCTATTGATCCTTTTGTTTCTATATTCTTTAGATATGTTGTTTCATTGGTTGTTTTAGGTAGCATTGTTTTACCATCAGCAAAAATCTATGAAAAAATGGATGAACTATTTGCTAATACAAACATTTTATTAATTGTTGCTTCTGGAATTGTGGGTTGCATTTCATATATGACATTCTATTATGCACTTGATAAAATAGGTGTTCCAATGGCTACAGGATTAAATATTTCCTATGTTGGTTGA", "aa": "MKNAIDPFVSIFFRYVVSLVVLGSIVLPSAKIYEKMDELFANTNILLIVASGIVGCISYMTFYYALDKIGVPMATGLNISYVG", "aa_hexdigest": "3e0fff2e79b66575fee525df8745586a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9090.746299999993, "isoelectric_point": 5.996877479553221 }, "id": "EFPKPENHBP_362", "locus": "GCA000186005_00323" }, { "type": "cds", "sequence": "contig_1", "start": 171891, "stop": 172109, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTATGACAATGTATGTCCTTGGTATTGTTTATGTCGGTACTGGAATAAGTAGTGCAATTACAGTTTGTTATCCTATTGTTGGCGCAATGCTTGCTTTTATCTTTTTGAAACAAAAAACTACAAAAAATGGTTTATTTGGATCAATAATTTCGCTTTTATGTATTTTAACTTTAGGAATTTTACAATTTCATAAAGGCGATATTAAAAATGGATGA", "aa": "MGMTMYVLGIVYVGTGISSAITVCYPIVGAMLAFIFLKQKTTKNGLFGSIISLLCILTLGILQFHKGDIKNG", "aa_hexdigest": "02f003578eb20d1715acdfe84f1f5df8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7686.295599999993, "isoelectric_point": 9.391705894470213 }, "id": "EFPKPENHBP_363", "locus": "GCA000186005_00324" }, { "type": "cds", "sequence": "contig_1", "start": 172350, "stop": 172460, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATAAAAATTCAAATGTAGAAGTTAATGAAATTCAAAACGATAAAAAAGTTAAAAATAAAAATTTATTTAATAAATTTGTTGCTTTATTTTCAGGGATTATGTGA", "aa": "MNDKNSNVEVNEIQNDKKVKNKNLFNKFVALFSGIM", "aa_hexdigest": "f5070d8a45990f166b5e2f6e68fd5c90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4171.753500000001, "isoelectric_point": 9.303642082214353 }, "id": "EFPKPENHBP_364", "locus": "GCA000186005_00325" }, { "type": "cds", "sequence": "contig_1", "start": 172589, "stop": 172798, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGA", "aa": "MYNLFFSSLVGFLCKYVSKSQLASLTPYSLSMHIPAKSKNGSLLAAYSQSINLILLSLFIKIFPEVESI", "aa_hexdigest": "1399650964bfb56fc1c382f6599735c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7664.005399999998, "isoelectric_point": 9.25226078033447 }, "id": "EFPKPENHBP_365", "locus": "GCA000186005_00326" }, { "type": "cds", "sequence": "contig_1", "start": 172732, "stop": 173007, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAGCTTTAAATAGTTATCGCAAACTACATAATCAAAATTTATCAGTTGATTTTGAATTTGATATTCTAAAAATTTTGGACTATTACATTTCAATAATTGGAGAAAAATACAAAAGATTTCCAAAACATTTATTAGACAATGAAGCTAAAATTAGAAAAATGCTTGCTTATCATAAAAGTAAAAATTATCCAAAAGTTTTATGTCATATTGATTCAACTTCAGGAAATATCTTAATAAATAAGGATAATAAAATTAAATTAATTGATTGA", "aa": "MELALNSYRKLHNQNLSVDFEFDILKILDYYISIIGEKYKRFPKHLLDNEAKIRKMLAYHKSKNYPKVLCHIDSTSGNILINKDNKIKLID", "aa_hexdigest": "d9c6b851be682b510d6594e446a48221", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10796.503999999984, "isoelectric_point": 9.32594814300537 }, "id": "EFPKPENHBP_366", "locus": "GCA000186005_00327" }, { "type": "cds", "sequence": "contig_1", "start": 173050, "stop": 173457, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATCTTAAAAGATATAGAGCTTAAGAAATACAATTGTGACCAAAAGGTCTATGAAATAGACGATTTCAAAGATTTATTGACATTTAATAGAAAAGCTCCTTGCTTTATAAACGATGTTTATTTAAACAAAATTAAAGAAGTTTTTAATGCCAAATTTGAAGATATTTTAGATATTCAACCAGTTAAAAAAGGAATGACTAATAACTCATTTTCTTTTAAAATAAAAGATAAAAAATATATTGCTAGAATACCAAAAGATGGCTCAAAGAGTTTAATTAATAGATACCAAGAAGAAAAAATTTATCAAAAACTAAAAGGCAATGAATTAGTTGAAAATTTAATTTATTTTGAAACCAAAAATAATATTGATGATCCTTTACATGGTTTTAAAATAACTGAATGA", "aa": "MDILKDIELKKYNCDQKVYEIDDFKDLLTFNRKAPCFINDVYLNKIKEVFNAKFEDILDIQPVKKGMTNNSFSFKIKDKKYIARIPKDGSKSLINRYQEEKIYQKLKGNELVENLIYFETKNNIDDPLHGFKITE", "aa_hexdigest": "5b961e929c2f8c8d42b547d59ec90c57", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16068.357999999991, "isoelectric_point": 8.37097797393799 }, "id": "EFPKPENHBP_367", "locus": "GCA000186005_00328" }, { "type": "cds", "sequence": "contig_1", "start": 173881, "stop": 174069, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAAATACTCAAATAATAATTTTAGCTGCTGGCTTTGGTTCAAGACTAACGCCTCTTTCACTTAAGACGCCAAAAGGATTATTAGAAATTAAAAAAGATTTAAAAATTGTTCCTCATCTTTTATCTTATTTAAAAGATTATAAAAATAAGACAATAGTAACTGGCTACAATTATCAATCTTGA", "aa": "MDKNTQIIILAAGFGSRLTPLSLKTPKGLLEIKKDLKIVPHLLSYLKDYKNKTIVTGYNYQS", "aa_hexdigest": "e210dcd2e2b6ed5bc32040c1362c3f38", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6993.214900000004, "isoelectric_point": 9.795277976989745 }, "id": "EFPKPENHBP_368", "locus": "GCA000186005_00329" }, { "type": "cds", "sequence": "contig_1", "start": 174413, "stop": 174697, "strand": "+", "frame": 2, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAA", "aa": "MGDYVIVVNAEKVILTAKKEEDKVYYHHSGYPGGLKSITAAKLRAKKPTALVEKAIYGMLPHTKLGNKQRKNLSVVAGPDHKHQAQKPESLEVR", "aa_hexdigest": "029cc29247d3ecb5cd02735fdeba0c5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.755, "score": 145.0, "evalue": 2.95e-43, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "EFPKPENHBP_369", "locus": "GCA000186005_00330" }, { "type": "cds", "sequence": "contig_1", "start": 174699, "stop": 175118, "strand": "+", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_013354416.1", "SO:0001217", "UniParc:UPI0001A8C0DC", "UniRef:UniRef100_C4XEB8", "UniRef:UniRef50_Q8R7Y9", "UniRef:UniRef90_C4XEB8" ], "nt": "ATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAA", "aa": "MATAKKVTKKEVVEYRGLGRRKSSTARVILRPGKGKFTINKREALAYLTSNLYIQDAEQPLVLTETKGQFDITVNVSGGGLSGQAGAIRLGIARALLAASDTYREKLKPAGMLTRDARVTERKKPGLNKARRARQFSKR", "aa_hexdigest": "6865a3611ec67d39576e69dc1c2da313", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C0DC", "ncbi_nrp_id": "WP_013354416.1", "uniref100_id": "UniRef100_C4XEB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0DC", "RefSeq:WP_013354416.1", "UniRef:UniRef100_C4XEB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEB8", "uniref90_id": "UniRef90_C4XEB8" }, "psc": { "uniref90_id": "UniRef90_C4XEB8", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q8R7Y9", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q8R7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8R7Y9" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "EFPKPENHBP_370", "locus": "GCA000186005_00331" }, { "type": "tRNA", "sequence": "contig_1", "start": 175175, "stop": 175251, "strand": "+", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 82.2, "nt": "GCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 175209, 175211 ], "id": "EFPKPENHBP_5", "locus": "GCA000186005_00332" }, { "type": "cds", "sequence": "contig_1", "start": 175403, "stop": 175513, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGA", "aa": "MKKDQLPYLPKNLKRRQQKSFELLKVLDKICRENNL", "aa_hexdigest": "00fb306c34e80ce74b54c09c2d458fbd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4429.2621, "isoelectric_point": 10.01279430389404 }, "id": "EFPKPENHBP_371", "locus": "GCA000186005_00333" }, { "type": "cds", "sequence": "contig_1", "start": 175883, "stop": 176110, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTTAGTACCACCTTTAGATATTAAAACAACTATTGAAAAAACTAAGGTTTCAGAAAAAGAAAAAGAAATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGA", "aa": "MFLFLVPPLDIKTTIEKTKVSEKEKEMYYITNWPNKRIFHQVTLTLEQIENGMQDIEMNGHYFKSLKENESVLER", "aa_hexdigest": "cf17c2726fbf78c83a8070c50a237ccf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9036.344099999998, "isoelectric_point": 5.637996101379394 }, "id": "EFPKPENHBP_372", "locus": "GCA000186005_00334" }, { "type": "tRNA", "sequence": "contig_1", "start": 176293, "stop": 176376, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 67.2, "nt": "GCCCAAGTGGTGGAATGGTAGACGCTGTGGACTCAAAATCCACTGGAGAGATCCGTGCAGGTTCAAGTCCTGTCTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 176371, 176373 ], "id": "EFPKPENHBP_33", "locus": "GCA000186005_00335" }, { "type": "rRNA", "sequence": "contig_1", "start": 176450, "stop": 176556, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8991596638655462, "score": 69.1, "evalue": 1.5e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TCCAGTGGTTATAGCGAAGGGGACCCACCTGATTCCATTCCGAACTCAGTAGTTAAGCCCTTTAGTGCCGACGATACCAGAGATGGGAAAATAGGGAGCTGCTATTT", "id": "EFPKPENHBP_40", "locus": "GCA000186005_00336" }, { "type": "cds", "sequence": "contig_1", "start": 176917, "stop": 177150, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGA", "aa": "MPIKISGFIFGPLIGGIVGLISDLLSFLFVPSFYNPIYTVAAMVDGIISGLVGLLFIKLFGYYFGGEFQDNVHENRI", "aa_hexdigest": "82836e21f88382467fca2d6588f01146", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8433.870399999996, "isoelectric_point": 4.8263353347778315 }, "id": "EFPKPENHBP_373", "locus": "GCA000186005_00337" }, { "type": "cds", "sequence": "contig_1", "start": 177424, "stop": 177654, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGA", "aa": "MVSGYTLMFFFIIIARFKMKPSNYLVIVPIVIFSALIELINVPLLSFADQQSLQKGEDNNIFVFVFNHILFSPVKI", "aa_hexdigest": "004f4485996cecc998465410b5c0913e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8741.412499999995, "isoelectric_point": 8.120389366149901 }, "id": "EFPKPENHBP_374", "locus": "GCA000186005_00338" }, { "type": "cds", "sequence": "contig_1", "start": 178898, "stop": 179014, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGA", "aa": "MKTMLSIALEYISEKSKDGKNIKFNDIFKEIKANLNDK", "aa_hexdigest": "15155089558628a0506e499f424d0955", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4419.124800000002, "isoelectric_point": 9.100760078430177 }, "id": "EFPKPENHBP_375", "locus": "GCA000186005_00339" }, { "type": "cds", "sequence": "contig_1", "start": 179191, "stop": 179280, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGA", "aa": "MKLVNASSMLKKAKKGKYAVPHININNLE", "aa_hexdigest": "63c020b77579d9ad77c92bd5bc852cc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3240.8828000000003, "isoelectric_point": 10.12516269683838 }, "id": "EFPKPENHBP_376", "locus": "GCA000186005_00340" }, { "type": "cds", "sequence": "contig_1", "start": 179359, "stop": 179745, "strand": "+", "frame": 1, "gene": null, "product": "class II fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "nt": "ATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGA", "aa": "MGGMNIIVGMINGMMKDYNITVPVALHLDHGTYEGAKKAIKAGYTSLMFDGSHLPYEENLAKTKELVKLAKKVNMSMEAEVGTIGGEEDGIIGAGEFADPAEAKEMAETGIDVLAAGIGNIHGPYPIS", "aa_hexdigest": "b8ece84c05e30ae815d3fcafb7083b00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0015C42C51", "query_cov": 0.9921875, "subject_cov": 0.8523489932885906, "identity": 0.504, "score": 132.0, "evalue": 4.97e-37, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_UPI0035C6842C", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_UPI0035C6842C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "product": "class II fructose-bisphosphate aldolase" }, "genes": [], "id": "EFPKPENHBP_377", "locus": "GCA000186005_00341" }, { "type": "cds", "sequence": "contig_1", "start": 180281, "stop": 180484, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "TTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGA", "aa": "MYFTNDTIKQGVRFFGIDTPEIFKGKNNQHLALLENYYGQKAKNYLYNLTYQKNIKIKYITKDKYNR", "aa_hexdigest": "39ea9fe130d16a562402197c5ffa17dd", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 56, "aa_cov": 0.835820895522388, "hmm_cov": 0.8928571428571429, "evalue": 1.4231756939533748e-09, "score": 34.69647979736328, "start": 12, "stop": 67 } ], "seq_stats": { "molecular_weight": 8120.213899999999, "isoelectric_point": 9.698124122619632 }, "id": "EFPKPENHBP_378", "locus": "GCA000186005_00342" }, { "type": "cds", "sequence": "contig_1", "start": 180899, "stop": 181834, "strand": "-", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_S5M1G4", "UniRef:UniRef90_A0A3P8K9P9" ], "nt": "ATGGCTCAAGACTTAGTTGTTGGAAACCAAGTTCTCTTTGACGATGGTAAATTATCAACAATTGTAACTAAAGTTGATAGAAAAGCTGGTTATGTTGTTGTTAAAACACTTAACAAGCACTTATTAAAAACTAACAAACGTATTAATTTACCAGGTGTTGACTTTTCTCTTCCATTTTTAGCTCAAAAAGACATTGATGATGTTAAATTTGGTATTAAAAACGGAATTAACTATGTAGCAGCATCATTTGTTAACTCATCTAAAAATGTCCAAGAATTACGTAAATTATTAGATGACAATGGTGGAAAACACGTTAAAATCATTTCTAAAATTGAAAGCCACTTAGGAACCAAAAACATTGATGAAATCATTCAAGCATCAGATGGTGTTATGGTTGCTCGTGGGGACTTAGGTCTTGAAATTCCTTACTATGATGTTCCTTATTTTGAAAAAATAATGATCAGAAAATGTCGTGAAGCGGGTAAACCAGTTGTTGTTGCAACTCAAATGTTAGACTCAATGGAAAACTCACCACAACCTACACGTGCTGAAGTTACTGACGTTTACTATGCAACAGAAATGGGTGCTGACTCAACAATGCTTTCAGGTGAAAGTGCATCAGGCAACTATCCTTTAGAAGCAGTTACAGTTATGTCAAAAATTAACAAACGTGCTGAAAGTGAATTTTATGACAAAAATTACTATGATGTTCAATTAGAAGAAATTAGAAAAAATTCTTCAAATTCAAAACGTTCAAGAATTGCTTATGACGTTGCAAACAGAACACGTTATGGTGACTATAGATTTGCTGTTGTTCTTTCAAGAACAGGTCAATTATTAAGTGAAGTTGCTAAATTTAGACCAAATACATTAATCGTTGGTATTTTAAATGATGAAAACCTAATCGGTGGATTTGGTGTTACTTCTAGTGTTTGA", "aa": "MAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSV", "aa_hexdigest": "1f4f1a05519381d88e406f276f439efe", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 180755, "stop": 182134, "observations": { "mutation": [ 180901, 181900 ], "stop": [ 180901, 181900 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K9P9", "query_cov": 1.0, "subject_cov": 0.6519916142557652, "identity": 1.0, "score": 601.0, "evalue": 2.09e-214, "gene_start": 1, "gene_end": 311, "reference_start": 119, "reference_end": 429, "reference_sequence": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKVWTTKEKYETVEGTEKELTVAYDMAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSVWVSLDSIELFAKIKKDKSKALNALKPYGVKKGDRFLVVENESITEFVA" }, "paralog": false, "identity": 0.9956427015250545, "score": 888.0, "evalue": 0.0, "description": "Internal stop codon at 180901, 181900. Nonsense mutation around 180901, 181900.", "psc": { "uniref90_id": "UniRef90_A0A3P8K9P9", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_S5M1G4", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K9P9", "EC:2.7.1.40", "UniRef:UniRef50_S5M1G4", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_S5M1G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5M1G4" ], "product": "Pyruvate kinase" } }, "id": "EFPKPENHBP_379", "locus": "GCA000186005_00343" }, { "type": "cds", "sequence": "contig_1", "start": 181898, "stop": 182188, "strand": "-", "frame": 3, "gene": null, "product": "pyruvate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "nt": "ATGAACATTACTGACAAAAGAAGTAAGTTAGTTATTACAGTTGGTCCATCAAGTGATAACTACAAAATGCTTCGTTCATTAATTGAAGCAGGTGCAACCTGTGTTCGTGCTAACTTTAGCCACGGAGACCATGCAGAACAATTAAACAAATTTGTTCTTGCACAAAAAATTTCTAAAGATTTACATCTTCCTATATCTCTTATGTTAGATACTAAGGGTCCGGAAATTCGTTTAGGAAAAATGAAAGATGGAAAACAAGAAATTCCAGCAAACAAAGAAATTAAAGTTTGA", "aa": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKV", "aa_hexdigest": "d00046a4307e35aa0ac1a254290185b4", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387B60A9", "query_cov": 0.8645833333333334, "subject_cov": 0.9540229885057471, "identity": 0.711, "score": 125.0, "evalue": 8.67e-36, "valid": false, "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_UPI00387B60A9", "kegg_orthology_id": "K00873" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B60A9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "product": "pyruvate kinase" }, "genes": [], "id": "EFPKPENHBP_380", "locus": "GCA000186005_00344" }, { "type": "cds", "sequence": "contig_1", "start": 182372, "stop": 182620, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTAAAGATGATTTTAAAAATAGTCACCTTTACCAATTATTCTATTGTACAAAACAAACAGAAGATTATGATAAAGACAAAACAAAATATAAAAATGAAGGTTTAGTTTCATTTGATCCAGCAAAAGGCGAAGCTACCTTTGCAATTGGTATTAGAGTTAAAAATTCAAATAATAATTATAAGTCTTCTTCAATTCCTGAATATATTGGACATATAACTTTAGGTGGTTTTAAAAAATCATAA", "aa": "MKFKDDFKNSHLYQLFYCTKQTEDYDKDKTKYKNEGLVSFDPAKGEATFAIGIRVKNSNNNYKSSSIPEYIGHITLGGFKKS", "aa_hexdigest": "ba99a65ba617ad9e85d4b70fba226503", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9440.507699999998, "isoelectric_point": 9.16303653717041 }, "id": "EFPKPENHBP_381", "locus": "GCA000186005_00345" }, { "type": "cds", "sequence": "contig_1", "start": 182690, "stop": 182887, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAGGACAAAAATGATAAAACATCTTTAATAAAATCAAGAATGATTGACATAAAAAATCCTATTGTTCAAGAAATAGATATTAATTATTTTGAACTTGAAAAAGTATTGAAAAATCATGTTGAAATATCTATTTTAGGTAACGATGAAATTAAAAATATTGACTTTAAAACACCTTCAACTTCACTTGAATGA", "aa": "MPKDKNDKTSLIKSRMIDIKNPIVQEIDINYFELEKVLKNHVEISILGNDEIKNIDFKTPSTSLE", "aa_hexdigest": "6b95fea2f551b33ff42349dbf176ccfc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7544.609099999998, "isoelectric_point": 5.330781364440917 }, "id": "EFPKPENHBP_382", "locus": "GCA000186005_00346" }, { "type": "cds", "sequence": "contig_1", "start": 183032, "stop": 183187, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTAGAGATAAAATAAGACAAACTTTTTACAAGAAAGAAAAGAGTAGTCTTGATGAATTACTTGCTGCATTACCACAAAAAGATCTTTCAAGTGATTTAAACAAAAATAAAGAAGAAATTTTAAAAAATAATTCAACAATTGAAAGTTGA", "aa": "MDIRDKIRQTFYKKEKSSLDELLAALPQKDLSSDLNKNKEEILKNNSTIES", "aa_hexdigest": "2fa731d2308752bb98229033ee3aa0b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5939.660099999998, "isoelectric_point": 6.175920295715332 }, "id": "EFPKPENHBP_383", "locus": "GCA000186005_00347" }, { "type": "cds", "sequence": "contig_1", "start": 183287, "stop": 183487, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAATCAATTATTTTCATTAAGAAATAATCGTTTTGATAGAATTGACAAATTTTATAAAGATTACAATATTTTCTACCATTCATATGCAAATGATTTTACAGGTACACTCTATTTGACTGTTTCATTAGAAGATAAAGCTAATGAAAAAGATAAAGATAAAAAAACAAGAAACATTAGTGATAGAATAAAAAACTGA", "aa": "MRNQLFSLRNNRFDRIDKFYKDYNIFYHSYANDFTGTLYLTVSLEDKANEKDKDKKTRNISDRIKN", "aa_hexdigest": "70cab7cf96435a1a43b11fa1e643cbbd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.936699999999, "isoelectric_point": 9.474483299255372 }, "id": "EFPKPENHBP_384", "locus": "GCA000186005_00348" }, { "type": "cds", "sequence": "contig_1", "start": 183557, "stop": 183853, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCGTAAATTATCATTAACATTAGGAACATTAGCAACAGGTGTTGTTGCAGTTGGAATTGGTACGGCTATTTATGCTTTAGTTAAAACTAATAAAGACAATAAAAGTATTGAAAGACAGCTAAGCTCTTTTACTTTTTCTCTTGCAAAACCTAAAGACAAATTATACCAAACTAATATTGCAAATCACTTAGCAAGTGAATTTACTTACTCAAATTATTACTATTTTGAAACATACAATAGCAACAAAGAACCAACTTTGAAAAACACAAAAGAATATGAAAAAAATTATTGA", "aa": "MNRKLSLTLGTLATGVVAVGIGTAIYALVKTNKDNKSIERQLSSFTFSLAKPKDKLYQTNIANHLASEFTYSNYYYFETYNSNKEPTLKNTKEYEKNY", "aa_hexdigest": "a5046db0ae9ce25ddb62516bcc737bcb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11177.487099999991, "isoelectric_point": 9.397894859313965 }, "id": "EFPKPENHBP_385", "locus": "GCA000186005_00349" }, { "type": "cds", "sequence": "contig_1", "start": 184108, "stop": 184239, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTTGCGGTTAAAGATTTTTTAAAAAATGAACCTTCTCCAAAACATATCTTTTTGCTTATAGATAGTCGAACAAGATTTACTAAAAATGATTTAGAAACCTTGACATTTTTAAATTCATTAAACTGA", "aa": "MNLAVKDFLKNEPSPKHIFLLIDSRTRFTKNDLETLTFLNSLN", "aa_hexdigest": "9a7b4fa6c0f92d811c625176db386597", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5035.772399999999, "isoelectric_point": 8.211225318908692 }, "id": "EFPKPENHBP_386", "locus": "GCA000186005_00350" }, { "type": "cds", "sequence": "contig_1", "start": 184500, "stop": 184928, "strand": "-", "frame": 2, "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "db_xrefs": [ "EC:2.7.7.3", "GO:0004595", "GO:0015937", "KEGG:K00954", "RefSeq:WP_013526711.1", "SO:0001217", "UniParc:UPI0001F59FC9", "UniRef:UniRef100_C4XEC9", "UniRef:UniRef50_A0A449A8S0", "UniRef:UniRef90_C4XEC9" ], "nt": "ATGATTAAAAAAGCAATTTTTCCAGGATCTTTTGATCCTATACACAAAGGTCATATATCAGTAATTGAAAAAGCACTAAAGCTTTTTGATGAGCTTATAGTAATAGTTTCAATTAACCCTGATAAAAACAATTTAGGTAATTTAGAAGAAAGATATCAATCAGTCAAAAAAACCTTAAAAGACTATAAAACAGTCACTGTGGTAAAAAATGAAAATGATTTGATAGCTCAAATTGCTAAAGAAAAAAATGTAAAGTTCCTAGTTAGATCAGCCAGAAATAATTTAGATTATGACTATGAGCTAGTACTAGCAGCTGCTAATCATCACTTAAATTCTGAGCTTGAAACTATACTTATAGTACCAGACTATGAAAACATAGACTATTCTTCAACTCTTTTAAGACATAAACAAAAATTAGGTAAAAAATAA", "aa": "MIKKAIFPGSFDPIHKGHISVIEKALKLFDELIVIVSINPDKNNLGNLEERYQSVKKTLKDYKTVTVVKNENDLIAQIAKEKNVKFLVRSARNNLDYDYELVLAAANHHLNSELETILIVPDYENIDYSSTLLRHKQKLGKK", "aa_hexdigest": "087e78ccb649164a0c260c2e363af15b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59FC9", "ncbi_nrp_id": "WP_013526711.1", "uniref100_id": "UniRef100_C4XEC9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59FC9", "RefSeq:WP_013526711.1", "UniRef:UniRef100_C4XEC9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEC9", "uniref90_id": "UniRef90_C4XEC9" }, "psc": { "uniref90_id": "UniRef90_C4XEC9", "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "ec_ids": [ "2.7.7.3" ], "uniref50_id": "UniRef50_A0A449A8S0", "kegg_orthology_id": "K00954", "go_ids": [ "GO:0004595", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A8S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A8S0" ], "product": "Phosphopantetheine adenylyltransferase" }, "genes": [ "coaD" ], "id": "EFPKPENHBP_387", "locus": "GCA000186005_00351" }, { "type": "cds", "sequence": "contig_1", "start": 184931, "stop": 185449, "strand": "-", "frame": 3, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef90_C4XED0" ], "nt": "ATGGGACTTACACCTTTAGCAGGAATTATGATGGGATCGCGTTCAGGCGATATTGATCCTTCAATTCATGAATTTATTAATGAACAACTTGGTTTAGATATTCACCAAATCACAGAAGCTCTTAACAAGAAAAGTGGGCTTTTAGGAGTTTCAGGTGTTTCAAGCGATATGCGTGATGTATGTAAAGCAGCAGACGAAGGCAATAAACGTGCTATTTTCACACTTGAATTGTATGCACAAAAAATAGCTGATTTTACCGCTAATTATGCAAACAAAATTGGCAACAAATTAGATGCTATTGTTTTCACTGCTGGTGTTGGTGAAAACACTCCTTCAATGCGTCAAATGGTAGTTGATAAACTACATTTTGCAAAGATTAAATTAGATGACAAAAAGAATAATTCTAAAATTGAAGATTATGCTTTAATTTCTACAAAAGATAGCGAAATCAAAGTATATGTAATTAGAACAAATGAAGAATTATTAATTGCAAAACATGCAATAAATTTGTATAAATAA", "aa": "MGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK", "aa_hexdigest": "43a499d540fc49d4941e831af02a5074", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6YLS3", "query_cov": 0.9651162790697675, "subject_cov": 0.8383838383838383, "identity": 0.518, "score": 145.0, "evalue": 5.34e-41, "valid": false, "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_R6YLS3", "kegg_orthology_id": "K00925" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 184931, "stop": 185749, "observations": { "mutation": [ 185623 ], "stop": [ 185623 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.4321608040201005, "identity": 1.0, "score": 333.0, "evalue": 2.17e-112, "gene_start": 1, "gene_end": 172, "reference_start": 227, "reference_end": 398, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9963235294117647, "score": 533.0, "evalue": 2.63e-193, "description": "Internal stop codon at 185623. Nonsense mutation around 185623.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "EFPKPENHBP_388", "locus": "GCA000186005_00352" }, { "type": "cds", "sequence": "contig_1", "start": 185621, "stop": 186124, "strand": "-", "frame": 3, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef50_UPI0028BDD23C", "UniRef:UniRef90_C4XED0" ], "nt": "ATGAGTAAGCAAAAAGTATTAGTTATTAATGCAGGAAGTAGTTCAATAAAGTTATCACTTTTTGATAAAGCTTCTTTAGAAATTATTGCAAGTGGTATTGCAGAGAGGATTACCTTATTAGAAGGTAAAATAACAATCAAATATAACGGTCAAAAATTTAACCGTGATGTAACAATGCCTAACCACACTGTGGCTGTTGAAAACATTTTAGAATTAATGTCACAAATTGGTTTAATTGACAATCCAAAAGAAATCGAATTAATTGGCTTTAGAGTTGTTCATGGTGGACCTTACTTTACAACAACAGTTAAATTGAATACTAAAGAAATTGATTTAATTGAAAAAGCTAAAATTTATGCACCACTTCACAATCCAGGTGCAGTTCAAGCCATGAAAGCATTTAAAAAAGTTATGCCACATGCCAAAATGAGTGTTGACTTTGATACAGCATTCCACACAACAATTCCAGATGTCAATGCAATTTATCCAATTCCATATCAATGA", "aa": "MSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQ", "aa_hexdigest": "b3d35d81c65fe847b2a9740e420e5e5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021AE5BBA", "query_cov": 0.9640718562874252, "subject_cov": 0.8256410256410256, "identity": 0.609, "score": 192.0, "evalue": 9.47e-60, "valid": false, "product": "Acetokinase family", "uniref50_id": "UniRef50_UPI0028BDD23C" }, "pscc": { "uniref50_id": "UniRef50_UPI0028BDD23C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028BDD23C" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 185318, "stop": 186127, "observations": { "mutation": [ 185623 ], "stop": [ 185623 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.41959798994974873, "identity": 1.0, "score": 327.0, "evalue": 9.51e-110, "gene_start": 1, "gene_end": 167, "reference_start": 2, "reference_end": 168, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9925650557620818, "score": 525.0, "evalue": 4.8e-190, "description": "Internal stop codon at 185623. Nonsense mutation around 185623.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "EFPKPENHBP_389", "locus": "GCA000186005_00353" }, { "type": "cds", "sequence": "contig_1", "start": 186134, "stop": 187087, "strand": "-", "frame": 3, "gene": null, "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526713.1", "SO:0001217", "UniParc:UPI0001A8C0E9", "UniRef:UniRef100_C4XED1", "UniRef:UniRef50_Q4A5B1", "UniRef:UniRef90_C4XED1" ], "nt": "ATGAAATTTACATCTTTAATTGAAAAAAGAATTAAAGCAATGAAAACTAAAAAAAGAATTGCTTTTATAGATGGTGATGATAGTAGAGCACTAGATACCATTAAACAATTAGAACAATTTCCTAATGTTGAATTAACTTTATTAGTTGAAACTGATGTTAAGTCAAAAACCAATGTTAAGTTTATAAACATTAATAAAGATAAACAAAAACAAAATGATTTAGCTCAAAAATATTTTGAATTAAGAAAAGGCAAAGAAACAATTGAACAAGCTCAAAGAAACCTAAAAACAAGACCTTTCTATGCTATGATGCTTTTAGCTACAGGTGAAGTTGATGGGGTAGTTGGCGGACTTAATTATGCAACTGCAGATATCTTAAGAGCAGCTTTTAAAGCTATAGGCGCTAGAGAAGGAGTAAAAACTATTTCTTCAGTTATGGTAGTACATAACGAAAAAAATACTTACTTCTTCAGTGACATTTCAGTAAATGTTAAGCCTGATCAAAAAGCTTTAGTAGAAATTGGACTTAATGCTGCTCAATTTGCTCAAAGCATGGGGATAGAACCTAAAATAGCCTTCTTAAGTTTCTCAACTAACTATTCAGCAAAAACACCTGAAAGTGAATTAGTACACAATGCTGTTGAAGAATTCAATACCATTTATCAAGGTACTAAAGCTATTGGAGAAATTCAATTAGATGCTGCTTTAGATTTAGAAGTTAGAAAAGCTAAATATAAATTAGATTACTATAAAGAACCAGCAAATACTTTAATTTTCCCTGATTTAGATGCTGGAAATATAGGCTATAAATTAGCTCAAAGATTAGGAAATTATGGGGCAATTGGACCTATTGTTGTTGGTACTAAAAAACCAGTCAATGATCTTTCAAGAGGTTCAAGTGTAAAAGATGTAATTAATACAATTTTAATCACTGCATTGCAGGCAGAGAAATAG", "aa": "MKFTSLIEKRIKAMKTKKRIAFIDGDDSRALDTIKQLEQFPNVELTLLVETDVKSKTNVKFININKDKQKQNDLAQKYFELRKGKETIEQAQRNLKTRPFYAMMLLATGEVDGVVGGLNYATADILRAAFKAIGAREGVKTISSVMVVHNEKNTYFFSDISVNVKPDQKALVEIGLNAAQFAQSMGIEPKIAFLSFSTNYSAKTPESELVHNAVEEFNTIYQGTKAIGEIQLDAALDLEVRKAKYKLDYYKEPANTLIFPDLDAGNIGYKLAQRLGNYGAIGPIVVGTKKPVNDLSRGSSVKDVINTILITALQAEK", "aa_hexdigest": "42d7ecc2928b49aab7ff94df45fca491", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C0E9", "ncbi_nrp_id": "WP_013526713.1", "uniref100_id": "UniRef100_C4XED1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0E9", "RefSeq:WP_013526713.1", "UniRef:UniRef100_C4XED1" ] }, "ips": { "uniref100_id": "UniRef100_C4XED1", "uniref90_id": "UniRef90_C4XED1" }, "psc": { "uniref90_id": "UniRef90_C4XED1", "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "uniref50_id": "UniRef50_Q4A5B1" }, "pscc": { "uniref50_id": "UniRef50_Q4A5B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5B1" ], "product": "Phosphate acetyltransferase" }, "genes": [], "id": "EFPKPENHBP_390", "locus": "GCA000186005_00354" }, { "type": "cds", "sequence": "contig_1", "start": 187392, "stop": 187484, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBJ3", "UniRef:UniRef90_A0AB32XBJ3" ], "nt": "ATGAATATTTTAGAATCAGTCAAAGACAAATATTTGATCGAAAAACAATTAGAAGTATCAAAAACAAATTTAAAAAATACAATTTGGGCATGA", "aa": "MNILESVKDKYLIEKQLEVSKTNLKNTIWA", "aa_hexdigest": "58f624c707146b32f95fdfa773846146", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0AB32XBJ3", "query_cov": 1.0, "subject_cov": 0.9375, "identity": 1.0, "score": 59.7, "evalue": 1.78e-11, "valid": true, "uniref50_id": "UniRef50_A0AB32XBJ3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBJ3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3550.1278, "isoelectric_point": 8.099308204650882 }, "id": "EFPKPENHBP_391", "locus": "GCA000186005_00355" }, { "type": "cds", "sequence": "contig_1", "start": 187548, "stop": 187802, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013526716.1", "SO:0001217", "UniParc:UPI0001A8C0EB", "UniRef:UniRef100_C4XED3", "UniRef:UniRef50_C4XED3", "UniRef:UniRef90_C4XED3" ], "nt": "ATGGATATTGAAGCTGAAAAAAAGAATAGAGAAGAAGAATTAAGAAAGATGACAAGAGATCAACTTGAAATCTTAATAACTGATATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCAATTAAAACAAAGAAAAAAAGATTAA", "aa": "MDIEAEKKNREEELRKMTRDQLEILITDISYKEILERNNSKDLDEAIEKFKKRYGFSVTIKQIIAILGIKKSTYYYQLKQRKKD", "aa_hexdigest": "b784ba13d9bee4bf5f79cb33046cb416", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C0EB", "ncbi_nrp_id": "WP_013526716.1", "uniref100_id": "UniRef100_C4XED3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0EB", "RefSeq:WP_013526716.1", "UniRef:UniRef100_C4XED3" ] }, "ips": { "uniref100_id": "UniRef100_C4XED3", "uniref90_id": "UniRef90_C4XED3" }, "psc": { "uniref90_id": "UniRef90_C4XED3", "product": "Transposase", "uniref50_id": "UniRef50_C4XED3" }, "pscc": { "uniref50_id": "UniRef50_C4XED3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED3" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_392", "locus": "GCA000186005_00356" }, { "type": "cds", "sequence": "contig_1", "start": 188203, "stop": 188352, "strand": "+", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "nt": "ATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGA", "aa": "MSRVGNSLYNREIEYFFSILKTEIFPNFCKRVKLLTFNELKNEIKEFID", "aa_hexdigest": "80df6f3186f2c7c96547632095695c59", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFF27A6", "query_cov": 1.0, "subject_cov": 0.8448275862068966, "identity": 0.6940000000000001, "score": 70.5, "evalue": 3.7e-15, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0009993DF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0009993DF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "product": "IS3 family transposase" }, "genes": [], "id": "EFPKPENHBP_393", "locus": "GCA000186005_00357" }, { "type": "cds", "sequence": "contig_1", "start": 188702, "stop": 189391, "strand": "-", "frame": 3, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "nt": "ATGAAAATCTTAGATGGAAAAACTCTTGCTCAAAAAAGAACTAAAGAACTCATTGCACAATTTAAGGATATAAAGGAAAAGTTAGGCAGAAAACCAATTTTATCAATTATTCAAGTTGGCGATAATCCCGCATCTACTCACTACATCAAAAACAAATTAAAGAAGGCTGAAGAATTAGGAGTAGAAGCAATTCATCATCATTTTCCAGCTGATATTAGACAAAAAGATTTGCTTAAGAAATTAGACATTATTAATGAAAAATCTGATGGTATTGTTATTCAACTTCCTTTACCTAAAGAATTTGCCACACAAGTTATTTTAGATAGCGTTCGCATTGAAAAGGATATTGATGGTTTATGTACCAGAAATGCATTCAATTTCTACAATCAAGCGGATGACTTTACCTTAGTTCCAGCTACTGCTATTGGTATTTTAGATTTATTAAATAGCTACAATATCAAAATGATTGACAAAAAAGCTGCTGTTATTGGTCGTTCAAGTTTAGTTGGAAAACCTACAGCATTTCTATTAAAACAAGAAGGTTGCAATGTTTCTACCTACAATAGAAACACAGGCATTAAAGGTGTTGAAAATGCTGATATCATTGTAGTTGCTGCTGGTGAAGCCAAACTTCTTAAAAAAGAAAACATTAAAGAAGGTGCTGTTGTAGTTGATGTTGGATCAAACTGA", "aa": "MKILDGKTLAQKRTKELIAQFKDIKEKLGRKPILSIIQVGDNPASTHYIKNKLKKAEELGVEAIHHHFPADIRQKDLLKKLDIINEKSDGIVIQLPLPKEFATQVILDSVRIEKDIDGLCTRNAFNFYNQADDFTLVPATAIGILDLLNSYNIKMIDKKAAVIGRSSLVGKPTAFLLKQEGCNVSTYNRNTGIKGVENADIIVVAAGEAKLLKKENIKEGAVVVDVGSN", "aa_hexdigest": "b0ba4cc88f8b47363debf5a24bf6dbee", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8L7E0", "query_cov": 1.0, "subject_cov": 0.8063380281690141, "identity": 0.873, "score": 403.0, "evalue": 1.38e-140, "valid": false, "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_L0RXG1" }, "pscc": { "uniref50_id": "UniRef50_L0RXG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "EFPKPENHBP_394", "locus": "GCA000186005_00358" }, { "type": "cds", "sequence": "contig_1", "start": 189388, "stop": 189963, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19", "PFAM:PF10458.14" ], "nt": "ATGCCAAATAAAAAAGCTGCTTTAGATAACCTAAAATCATTACTTTTAATAGCTCATCCATTTTTACCTTTTATTACTGATCACTTATATAAAGTACTATTTAACAAAGAATTATTAGCTCAAGAATTTCCAAACTTAAAGAATCAAAAAGTTGAAAATCTTGAACAAATTGAACAAATAATTTCTATTGTAAATATACTTAGAAAATATCGTGAAGACAATAATATTAGTAAAAAAGAAGTGCTTTATTATGACACTAATATTAAACAAAATGAGTTTGTTAAAAATTCACTAAAATCACTATCAAACAGTGAAATTAGTGAAAATAAAGACACTTTATTTGTTGATGGAAATATTAATGTTTATATTAAAGAATCAGCCGAGCAAAAAAACACTTACATTGCTAAATTAAAAGAGAAAATAGCTAATTTAGAAAGCGAAGTTAAACGTTCAAACAATATTTTAAGTAATCAAAACTTTATAGCTAAAGCACCTACATCTAAAGTTGAAGAAGAAAAACAAAAACTAGCTAAATATCAAGAAGAATTAGACAAATACAAGGAGGAGTTAAAATGA", "aa": "MPNKKAALDNLKSLLLIAHPFLPFITDHLYKVLFNKELLAQEFPNLKNQKVENLEQIEQIISIVNILRKYREDNNISKKEVLYYDTNIKQNEFVKNSLKSLSNSEISENKDTLFVDGNINVYIKESAEQKNTYIAKLKEKIANLESEVKRSNNILSNQNFIAKAPTSKVEEEKQKLAKYQEELDKYKEELK", "aa_hexdigest": "11acec314f31c8c1d10b522c436ddeb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 78, "aa_cov": 0.3769633507853403, "hmm_cov": 0.9871794871794872, "evalue": 3.2284865816842954e-14, "score": 49.35884475708008, "start": 9, "stop": 80 }, { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 58, "aa_cov": 0.3036649214659686, "hmm_cov": 1.0, "evalue": 9.031386634413257e-13, "score": 44.672828674316406, "start": 134, "stop": 191 } ], "seq_stats": { "molecular_weight": 22322.269500000017, "isoelectric_point": 8.473611640930176 }, "id": "EFPKPENHBP_395", "locus": "GCA000186005_00359" }, { "type": "cds", "sequence": "contig_1", "start": 190261, "stop": 190425, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTTTTTGGATTAGAATTCATGAAACAAAAACCTTTTGATAGAGTATTAATTCATGGCCTTGTTAGAGACCAATTTGGTAAAAAAATGTCTAAATCATCAAACAATGGTGTTGATCCAATTCAAGTTATAAATGAAAATGGTTCTGATGCTTTAAGATGA", "aa": "MYFFGLEFMKQKPFDRVLIHGLVRDQFGKKMSKSSNNGVDPIQVINENGSDALR", "aa_hexdigest": "91455ff6f6a1dbabfc0de21e00dfe881", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.8566315474635373e-18, "score": 62.12314224243164, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 6204.080200000002, "isoelectric_point": 9.300805473327635 }, "id": "EFPKPENHBP_396", "locus": "GCA000186005_00360" }, { "type": "cds", "sequence": "contig_1", "start": 190699, "stop": 190794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAATTAGCTCAAGATATTTTAGACCACTTAAAATCAAAAGATGCAGTTAACTTTTATCCAAAAAGATTTGTTAATGTTATCAAAAAATGA", "aa": "MDKLAQDILDHLKSKDAVNFYPKRFVNVIKK", "aa_hexdigest": "76a673df5861ddef108e6deeff302e9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3675.3042000000005, "isoelectric_point": 9.601679420471193 }, "id": "EFPKPENHBP_397", "locus": "GCA000186005_00361" }, { "type": "cds", "sequence": "contig_1", "start": 190804, "stop": 191286, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ], "nt": "ATGTACTATATCAAGTATCCAATCTTGAATTCTAATAAATATTTAACAGTTGCCACAGTTAGAACTGAAACTATGCTTAGTGACGTTGCTGTTATTTACAATCCAAAAGACAAAAGATATAAAGATTTTAAAGACATTCAAATTGTTCATCCTTTAACTAAACAAATAATTCCATTTGTGCCTGATGAATATGCAGACATGAAATTTGGTAGTGGCTTAATGAAATTATCAGCTCATGCTGAAGTTGATATTGACATTATCAAAAAACACAAATTACAAATTAATGAAACTATTGATAAAGACGGTTTAATTAACTGTCCAAAAAGTCAATTCCACAAATTAACTCGTCAAGAAGCAAGAGAAAAAATTGGTCAATATTTAGCTGATAATAATTTATTAGTTAAAGTAGAAACCATTACTTCAAATGTTGGTTATTCAGAACGTTCAAATACTCCAGTTGAAACTCTTGTTATGCCACAATGA", "aa": "MYYIKYPILNSNKYLTVATVRTETMLSDVAVIYNPKDKRYKDFKDIQIVHPLTKQIIPFVPDEYADMKFGSGLMKLSAHAEVDIDIIKKHKLQINETIDKDGLINCPKSQFHKLTRQEAREKIGQYLADNNLLVKVETITSNVGYSERSNTPVETLVMPQ", "aa_hexdigest": "4df4a5302e1b8becaf4a3335513dd746", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF093D7", "query_cov": 0.84375, "subject_cov": 0.8709677419354839, "identity": 0.593, "score": 161.0, "evalue": 5.81e-48, "valid": false, "uniref50_id": "UniRef50_UPI002FF093D7" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF093D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18421.091699999994, "isoelectric_point": 8.502171134948732 }, "id": "EFPKPENHBP_398", "locus": "GCA000186005_00362" }, { "type": "cds", "sequence": "contig_1", "start": 191530, "stop": 191640, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGATCATGCTGGTATCGCAACTCAAAGCAAAGTTGAAAATACAATTTATCAAAAAGAAGGATTAAACAGACATGATTTAGGAAGAGAGAAATTTCTTAAACGTGTTTGA", "aa": "MDHAGIATQSKVENTIYQKEGLNRHDLGREKFLKRV", "aa_hexdigest": "4580192047fb739f6a76c75b47c368e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 1.384271777526599e-07, "score": 26.926347732543945, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 4183.7084, "isoelectric_point": 9.396476554870606 }, "id": "EFPKPENHBP_399", "locus": "GCA000186005_00363" }, { "type": "cds", "sequence": "contig_1", "start": 192060, "stop": 192263, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTTCTGATTCATTGGTTGAATCACAAGAGTTTGTGAAAACAAATACAAAAAATAAAAAGCATTTAAGACGGAACTTAATATGCTTATTGAACTTAAGAAATAGACCATTTTTAAAGTTGGTTTATTTTTTAAAACAATTGTTTTATAGTGATTTTGAAAAATTACAGGGATTAAAAATGGATGATAAAAAGATCTAA", "aa": "MEFSDSLVESQEFVKTNTKNKKHLRRNLICLLNLRNRPFLKLVYFLKQLFYSDFEKLQGLKMDDKKI", "aa_hexdigest": "311a3de9d2fae3a25f6669830a300332", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8154.533599999995, "isoelectric_point": 9.721655082702636 }, "id": "EFPKPENHBP_400", "locus": "GCA000186005_00364" }, { "type": "cds", "sequence": "contig_1", "start": 192250, "stop": 192393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCATCGTCAAGAGATTTCATTTGTTAAAATGTTTGAAAATATTTTACATATTAACGGAAGCAAAAATTATTATGTAGCTACATTGTTATATTTAATATTTAATTTTGTTTTAGGTTATGCAATTCATGGAATTTTCTGA", "aa": "MSHRQEISFVKMFENILHINGSKNYYVATLLYLIFNFVLGYAIHGIF", "aa_hexdigest": "5c39bea7bddd000749e6b6aa04520755", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5553.4569999999985, "isoelectric_point": 8.157845497131351 }, "id": "EFPKPENHBP_401", "locus": "GCA000186005_00365" }, { "type": "cds", "sequence": "contig_1", "start": 192928, "stop": 193119, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACACAAATTCAATATTTTTTGTATAATTTTAAGCATGGATACAATGTCAAAATTTTATTTAAAATTTAATACTGAATTTGCAAATGGAAGACCAGAAGCTTTTAGCGCATTCCACTTGACATTTTTAGCAACATTTCTTATTTTAGGCATCATTTTATGTGTTGCTTTAAGAAATAGAAAAGAATGA", "aa": "MGHKFNIFCIILSMDTMSKFYLKFNTEFANGRPEAFSAFHLTFLATFLILGIILCVALRNRKE", "aa_hexdigest": "73c5e07f6cf408f0ac09f2c2f8fe65fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7292.634699999997, "isoelectric_point": 9.236852836608886 }, "id": "EFPKPENHBP_402", "locus": "GCA000186005_00366" }, { "type": "cds", "sequence": "contig_1", "start": 193197, "stop": 193505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGCTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATATTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCAAGAAAATATGATGAATTTATCGATGCTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGATACAAGAGTTTGA", "aa": "MAEAKKKTTAANKAKKPVATKTTKKVTAAKTIKKPAATKAKVAKKPTLDKYYISEKPIKKTGDIEFNFKKAKQKTSRKYDEFIDALKEFIFVSETSKNDTRV", "aa_hexdigest": "19970ec1eb8ce9ec7cfbfc635afd95e5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11472.35979999999, "isoelectric_point": 10.115041160583491 }, "id": "EFPKPENHBP_403", "locus": "GCA000186005_00367" }, { "type": "cds", "sequence": "contig_1", "start": 193752, "stop": 194102, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTACTGAAGAAATAATGACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGA", "aa": "MLASEVQKDDIVASTPTRGCEVFVTNIYPSNGQMEVEYVVKNENNQSDSQIKTLYGFKQNPVTEEIMTISDEEEKKLESNIVEDTEPAEVVDVESDENKNTSVENDKKSKMSKELL", "aa_hexdigest": "fcad94414cc4237ce97bcef126f916cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13095.280099999996, "isoelectric_point": 4.27033634185791 }, "id": "EFPKPENHBP_404", "locus": "GCA000186005_00368" }, { "type": "cds", "sequence": "contig_1", "start": 194327, "stop": 194668, "strand": "-", "frame": 3, "gene": null, "product": "Integral membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9", "UniRef:UniRef90_C4XED9" ], "nt": "ATGATATTTATTCCAAATGAAGTCTTAAATAAATCGATATTTTTTGATTGTCACTCAATGATTCATCATGGAATTTTAATGTTAATTGGTATTGTTTTAATTTTTAATAATTATTCAAAATTCGACAAAGGAAATTATATTATTCATAATCTTTTTATGTTCCTAATAATGTTTAGTGTAGTAGTAATATTGAATGAAATTTTTTATCAAACAGCACACAAAGACTTAAATAAATTACAAGAAAATTATCCAAATTTATTAGCAATTAGTCATCATCTTAATAATCATTTGACACTATTATTTGAGAAAATTTTTAGTGTTAAATTAAATGGCAACTACTGA", "aa": "MIFIPNEVLNKSIFFDCHSMIHHGILMLIGIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHHLNNHLTLLFEKIFSVKLNGNY", "aa_hexdigest": "59a1d99c492e5aadd43b78defb7acdf7", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [], "truncated": "both", "pseudogene": { "start": 194204, "stop": 194842, "observations": { "mutation": [ 194329, 194806, 194824 ], "stop": [ 194329, 194806, 194824 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED9", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 1.0, "score": 225.0, "evalue": 2.27e-73, "gene_start": 1, "gene_end": 113, "reference_start": 59, "reference_end": 171, "reference_sequence": "MDKDNVWYSKYKWSYFPLFFCSIPYFLFPIYLINQNKNLNKILIDFVSFTSLYGGISIMIFIPNEVLNKSIFFDCHSMIHHGILMLIGIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHHLNNHLTLLFEKIFSVKLNGNYWVITLLYPVINFVLALAVYSLIIIIKVSNKIPENKNKMSFQ" }, "paralog": false, "identity": 0.9811320754716981, "score": 416.0, "evalue": 8.55e-151, "description": "Internal stop codon at 194329, 194806, 194824. Nonsense mutation around 194329, 194806, 194824.", "psc": { "uniref90_id": "UniRef90_C4XED9", "product": "Integral membrane protein", "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED9", "UniRef:UniRef50_C4XED9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9" ], "product": "Integral membrane protein" } }, "id": "EFPKPENHBP_405", "locus": "GCA000186005_00369" }, { "type": "cds", "sequence": "contig_1", "start": 195131, "stop": 195406, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGCCATAAAAATGAAAATAATTCTTTATTAGTAACTGATGAATTGAGTCAAGAAAAAACTATTGAAACACAAATAAATGATTTAGAGAATCCTTTATTAGAAAAGACAATTATGATAGCTGCTTTCCAGCCTAATAAAATTAAAAATAACAAAAATTTGGATAAAACATTTACTCAAGAATTAGAAGCTATTCAAAAAGTAAATAATAGTAATCAAAAAGTTGAAACTGACTTGACAATGACTCAAGTATTATCAGATGACATATATTAA", "aa": "MQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY", "aa_hexdigest": "f1d9a50958cafc651587f3115446cffd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10449.592199999994, "isoelectric_point": 4.6114839553833 }, "id": "EFPKPENHBP_406", "locus": "GCA000186005_00370" }, { "type": "cds", "sequence": "contig_1", "start": 195407, "stop": 196000, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGACTTTGTATTTAATAATGTTATTGTTCTTAAAAATAGTTTAGTTTTAGCTAAAAAAGTTTCTAATAAAATTCTTCACAAATTAGACCAATTTGACAGATTAATTGAAATTTTTGAAAAATCATCAATCAATACAAAGAGCTTAATTAGACCTTATGAAAATATTTCAAAAATTAACAAACTAAAAATGAACTTTATTTCTTCTATTATTAAGAAACCAAAACTAATTATTATTGCTGATGATCCTGATATTAAAGATACAAAACTTAAATTTGAATTTTTAAGAGCTGCTTTTGAATTATGTGAACAAAGTAAAATAAATATTATTTTTGTTACTCAAGATAAAGAATTAGTTGAAGACAATGAGTTTGACTATATCTATTTGATTGCTAATAATAAAGAAGTTGAATTTGGAGATTTAAAAGAAGTTTTAAATAACACAATTAACCCTGAAATTAAAAGTAAAATTAATGACCAAGCCATTTCACAAGAAATTATTAGAAGTCAAAAAGAATTTGTTTTTAGTGATATTTATGATGTAGATGAAGAGCACTACATTATTACACCTTCAAAATATTTCAAATTATGA", "aa": "MFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDTKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKL", "aa_hexdigest": "459813f0047f9350c5f0921f759b0fd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23178.578100000006, "isoelectric_point": 5.581043434143066 }, "id": "EFPKPENHBP_407", "locus": "GCA000186005_00371" }, { "type": "cds", "sequence": "contig_1", "start": 196232, "stop": 197314, "strand": "-", "frame": 3, "gene": null, "product": "MAG1360 family OppF-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97", "UniRef:UniRef90_C4XEE0" ], "nt": "ATGGCTGAAAAAGAAGAACAAAATTTACAATATCCTTTAGTTTACAAATATGAAGAACTTAAAAAAAATTACGGTGAAAACAAGGAACGTATTAACCAATTAAATAAGACAGTTAATAAATACTATTATCCTGTTCGTCATGTTATTTTGAATATTTTAGTAGAACAAGCTAACTCATTAATGGCGATTTTTCGTGATTTTGAAAGAAACTTCAATAGTTTTTTAAATAAAACAACAAAAAATATTGCTGAATATAACTTTGATGAATTACAAACAAGATTTAAAGAAATTGAAAGCTACCAAGACTTATTTTTTGGTGAACAAATTACTTTAATATTAGACTTGTTTTCTCAAGTATTTAATAAATTTGAATTCTTTTATAATGCATCTAAATCAAATATTGACTACTTGGGTGATGAACAAATAAATCACTTAAAAAAGAGACTTTACTACTTAACCAAAATGAGAAAAACTTCTAAAAAAAGAGTGGAAGACAACTTAAAAATTAGAGACTTAAAACATGATATTTGTGCTTTAGATGAAATAGCAAAAGAAATTTGTCACAACTCAGATCAAAAAATTAAATACTTTGCTTTTAGATATAAAAGAAGTGCCGAAATTGATTTGTATAAATCAACTTTTTTTGCTAAAGAAAGCTCACAAAGACAAATTTTAAACAAAAGATATTTTGTTAAAATGTTTGCTCACAAGTTACTTAAAAAATATAAAAAATATTTTAAATATTTAAAAATTGATGAACTTGATGAAATGAGAAGAACACTAGATACCACCATCAAATTATTTATTAGCAATAGTTTAGGAAATTTAACTTACAGCAATAAAAACTTTTCAATTAAAAAAATTAAAAAGATTCTTAAAGAAGAATTCTACTTTAATGTCGAAAGTTATAAAATCAAATCAAAAAGCAATTTTGACTTAATTAAAGATTCAAAAGATCAAATTCAAAGTCAAATAATGTCTATTAAAAATAGATCATATGGAACATACTTCAATACTGTCCCTGAAAGATCTATCAAAGTTGCAAAAGAAATTTTAACTAATGCTCAAAGTGAACATAATTGA", "aa": "MAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHN", "aa_hexdigest": "60d9e0ab51f4738d19f5480ecea7d40e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 195929, "stop": 197614, "observations": { "mutation": [ 196099, 196234, 197371 ], "stop": [ 196099, 196234, 197371 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE0", "query_cov": 1.0, "subject_cov": 0.43373493975903615, "identity": 1.0, "score": 659.0, "evalue": 6.07e-231, "gene_start": 1, "gene_end": 360, "reference_start": 104, "reference_end": 463, "reference_sequence": "MLFINMKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNYWNIEFNKNNNAFSEINFFNMAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHNWKFNKIQSEFYNTLKSNQKLLNSYDTMTLINSKIKRKIMKLKTSLWFKRIFNRSINEKDKLNIKNFSANLKKIIEKFEMFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDTKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKLWMQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY" }, "paralog": false, "identity": 0.9875222816399287, "score": 1049.0, "evalue": 0.0, "description": "Internal stop codon at 196099, 196234, 197371. Nonsense mutation around 196099, 196234, 197371.", "psc": { "uniref90_id": "UniRef90_C4XEE0", "product": "MAG1360 family OppF-related protein", "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE0", "UniRef:UniRef50_A0A3P8LI97" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97" ], "product": "ABC transporter ATP-binding protein" } }, "id": "EFPKPENHBP_408", "locus": "GCA000186005_00372" }, { "type": "cds", "sequence": "contig_1", "start": 197369, "stop": 197608, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAATAAAATATTTGGAATTGAAAATTGTTTTAAGAATCTAAATTTTGAAGATGATTATGTAGATTTACAAAAAATTGAGATTTACGATCAACTTAAAACTGCAATTTTAGTTACTGATAAAAACAGAAACCTAATTGGTTCCAATTTTTACAATACAATTTTCAATAACAATTTGAACATTTTATCTTTCTTTTCTAAGAATAATAAAAAAGGTTCAGGAACAAATTATTGA", "aa": "MKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNY", "aa_hexdigest": "7fde6c4fb76b82243f2d789bc966aacc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9251.382599999994, "isoelectric_point": 9.068719291687014 }, "id": "EFPKPENHBP_409", "locus": "GCA000186005_00373" }, { "type": "tmRNA", "sequence": "contig_1", "start": 197675, "stop": 198052, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 197758, "stop": 197847, "aa": "AEDKKAEEVNISSLMIAQKMQSQSNLAFA", "nt": "GCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAG" }, "nt": "GGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGACATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCA", "id": "EFPKPENHBP_37", "locus": "GCA000186005_00374" }, { "type": "cds", "sequence": "contig_1", "start": 198181, "stop": 198282, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGA", "aa": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNF", "aa_hexdigest": "6d44cb23e3ab1d5550544ec4465c6797", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3692.2652000000007, "isoelectric_point": 8.140632438659669 }, "id": "EFPKPENHBP_410", "locus": "GCA000186005_00375" }, { "type": "cds", "sequence": "contig_1", "start": 198457, "stop": 199557, "strand": "+", "frame": 1, "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "KEGG:K03601", "SO:0001217", "UniRef:UniRef50_C4XEE1", "UniRef:UniRef90_C4XEE1" ], "nt": "ATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGTTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAAGAATTAGTTAAAAGCATCAAAGAATTATTCTTACAAAAAATACAAGCAAAATTTGAAATGGCTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAA", "aa": "MNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIVESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFKELVKSIKELFLQKIQAKFEMAKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK", "aa_hexdigest": "a435a6d89cfe0e56660df2c5e13f9299", "start_type": "ATG", "rbs_motif": null, "genes": [ "xseA" ], "truncated": "5-prime", "pseudogene": { "start": 198181, "stop": 199557, "observations": { "mutation": [ 198280, 198292, 198382 ], "stop": [ 198280, 198292, 198382 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE1", "query_cov": 1.0, "subject_cov": 0.7991266375545851, "identity": 1.0, "score": 624.0, "evalue": 1e-222, "gene_start": 1, "gene_end": 366, "reference_start": 93, "reference_end": 458, "reference_sequence": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNFWQTYWIKASISKLSIKNHAYLNIVDGEYSSNAAIWASKIPNIGIDITSNNKDLYSHAYFMNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIVESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFKELVKSIKELFLQKIQAKFEMAKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK" }, "paralog": false, "identity": 0.9934497816593887, "score": 808.0, "evalue": 9.9e-298, "description": "Internal stop codon at 198280, 198292, 198382. Nonsense mutation around 198280, 198292, 198382.", "psc": { "uniref90_id": "UniRef90_C4XEE1", "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE1", "kegg_orthology_id": "K03601", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE1", "EC:3.1.11.6", "UniRef:UniRef50_C4XEE1", "KEGG:K03601", "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE1" ], "product": "Exodeoxyribonuclease VII large subunit" } }, "id": "EFPKPENHBP_411", "locus": "GCA000186005_00376" }, { "type": "cds", "sequence": "contig_1", "start": 199567, "stop": 199755, "strand": "+", "frame": 1, "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "RefSeq:WP_013354436.1", "SO:0001217", "UniParc:UPI0001E32FD5", "UniRef:UniRef100_C4XEE3", "UniRef:UniRef50_C4XEE3", "UniRef:UniRef90_C4XEE3" ], "nt": "ATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAA", "aa": "MSEKLKFNQIMNQIDEISNKLGSNIDLEDSINLYKQAEKLIEEAETKLVEVRKIVAKESIKQ", "aa_hexdigest": "bb796ea96a719634f36991ffb4c5135f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FD5", "ncbi_nrp_id": "WP_013354436.1", "uniref100_id": "UniRef100_C4XEE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FD5", "RefSeq:WP_013354436.1", "UniRef:UniRef100_C4XEE3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEE3", "uniref90_id": "UniRef90_C4XEE3" }, "psc": { "uniref90_id": "UniRef90_C4XEE3", "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE3", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE3" ] }, "genes": [ "xseB" ], "id": "EFPKPENHBP_412", "locus": "GCA000186005_00377" }, { "type": "cds", "sequence": "contig_1", "start": 199930, "stop": 200229, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGCGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGA", "aa": "MKQIFANDTNSIKRIDVQKMYASKHKKLVNNLLDIKSKGDNATFFKTSNLSLDLKKLIGIKNLREILNNVEFKIALKNSFDSKKLNELKNINTVSQFEV", "aa_hexdigest": "2eef6b667d819f30d1c02bf12a9934cd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.177699999987, "isoelectric_point": 9.901650810241698 }, "id": "EFPKPENHBP_413", "locus": "GCA000186005_00378" }, { "type": "cds", "sequence": "contig_1", "start": 200626, "stop": 201576, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTGCTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTATTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGA", "aa": "MTEALNSIKNIDSKYLNNLDYKETIFQEPFAEYSKVQLENNGCVELFPGTVLIEANPGGTKLREAMINLILNNKIDNLIEIDDLKDYTKTAYEKALGKGNIARVTPSDFSQEKAIIGTLNNSAIIIGPPGTGKSQTIANILVNIAKENKKALFISQKKVALDVVLKRLGKYQNMMFQFSETAKVNQSEKEYFYKPIIEYFKEINEVQENHDISKYNSSFFSKGEFKYLDAKSEIGEIFSKDLNCYFNLKNKGKIEQNIPKFIQFYKLYNELFSKNKFLKIFNNYKTLSKREIAYKTGYLKKYATIFTKLPAEFKRL", "aa_hexdigest": "1210eea2f1b59b6bc06462f9fd20d031", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 74, "aa_cov": 0.20253164556962025, "hmm_cov": 1.0, "evalue": 5.619162596076349e-10, "score": 35.706966400146484, "start": 109, "stop": 172 } ], "seq_stats": { "molecular_weight": 36422.52059999997, "isoelectric_point": 9.223701286315915 }, "id": "EFPKPENHBP_414", "locus": "GCA000186005_00379" }, { "type": "cds", "sequence": "contig_1", "start": 201649, "stop": 202116, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "ATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCGGCCAAGTAACTGA", "aa": "MSNFIRLNSFIEFDKNEKDYNKFLETKKSDNTTPELLEMIYENNAFEAKKRLDDYRQKSEAQRQKLNKFFGRLERKITPPYIISSLFKDIIKNVYNIYVGTPEILGNFIDFENDHYDYVIFDEASQIYIEKAIPFISIADKVIVAGDNQQMRPSN", "aa_hexdigest": "2499eac8e59a76e53372315dd534dd17", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 139, "aa_cov": 0.8903225806451613, "hmm_cov": 0.8920863309352518, "evalue": 8.543484256685015e-10, "score": 35.11067199707031, "start": 16, "stop": 153 } ], "seq_stats": { "molecular_weight": 18373.580899999986, "isoelectric_point": 5.314525413513183 }, "id": "EFPKPENHBP_415", "locus": "GCA000186005_00380" }, { "type": "cds", "sequence": "contig_1", "start": 202198, "stop": 202356, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGAGGGTCAATGA", "aa": "MPKYLLEMNYRSNASELVLFSSKEFYESGLKGLDNYPRKEKTSVEVIDVEGQ", "aa_hexdigest": "1747e62b2a8fd188a931bb4f8e573a63", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6033.747500000001, "isoelectric_point": 4.9316011428832995 }, "id": "EFPKPENHBP_416", "locus": "GCA000186005_00381" }, { "type": "cds", "sequence": "contig_1", "start": 202390, "stop": 202761, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGA", "aa": "MIQVCEKNIKKYNSIILLAFNRKQQDYIREQIALNSPKIFAELEDKVILRNIENIQGDEAELVIASVGYTGKTALHSTYIGSKKGRNALNVAITRAKDKMIIIKSIKTSEVDAKSENLMTFKR", "aa_hexdigest": "224945089dfb186540a8e58bbdf3938f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.8969072164948454, "evalue": 2.8769234094773247e-12, "score": 42.88240432739258, "start": 7, "stop": 103 } ], "seq_stats": { "molecular_weight": 13944.106899999977, "isoelectric_point": 9.575053977966306 }, "id": "EFPKPENHBP_417", "locus": "GCA000186005_00382" }, { "type": "cds", "sequence": "contig_1", "start": 202951, "stop": 203157, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAA", "aa": "MDNKSQFVVGLNVDSSSDDIEIDDFIEKRIKTDFMSVKGYPIYRVSNFRFAENQPSIYRFLNTLISKA", "aa_hexdigest": "6cac69579f4fd64bfaa62e3c79a728c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7914.845199999997, "isoelectric_point": 5.161571693420409 }, "id": "EFPKPENHBP_418", "locus": "GCA000186005_00383" }, { "type": "cds", "sequence": "contig_1", "start": 203564, "stop": 203932, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGCTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGA", "aa": "MLAAATAITLGSLYGCAKNSDEKLGKAHTGNPKDFKNYFVSVDKAESNFVTTDKSKKVAKFDFAKNQVIKENGQAISVDEFLDQYYREHHNLPFLNIKYGSFNFYNEYIEAVSPLEFQKFTN", "aa_hexdigest": "9d12f38d8e66c3a7f8be3ad04c7937f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13865.381299999992, "isoelectric_point": 6.824737358093262 }, "id": "EFPKPENHBP_419", "locus": "GCA000186005_00384" }, { "type": "cds", "sequence": "contig_1", "start": 204005, "stop": 204313, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGA", "aa": "MHGNSITLGAHSSKNKELTTIKFYPDAFFGTLPIYSELSGAGNAQDSLVYKVNQKLLTSEQLKDFLANISNYNAISNYSKTTLDNYGFRNLLDYRTLINQKV", "aa_hexdigest": "d236ab7c6e2c75a156e90a3fe3e31119", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11459.742699999986, "isoelectric_point": 8.860808753967284 }, "id": "EFPKPENHBP_420", "locus": "GCA000186005_00385" }, { "type": "cds", "sequence": "contig_1", "start": 204587, "stop": 204685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGA", "aa": "MDSYLNITFEDGTSFDVFNNFEDITHKNPKNE", "aa_hexdigest": "cb32795d8e0fccf327f9b3ecb853fab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3783.0048000000006, "isoelectric_point": 4.279487419128418 }, "id": "EFPKPENHBP_421", "locus": "GCA000186005_00386" }, { "type": "cds", "sequence": "contig_1", "start": 205943, "stop": 206365, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAATAA", "aa": "MISKRIKGLFTPFITKNETLPENYLILGNHGDYPLTKFNIIEKFDQYDSKNILEYIDIVYNGLSTATNPHHKEGYKKYYTRIEEKIKAGDATAFNLYSQLKALQVKRFEIIEKQNDPKEDKTLLQAQLLSITYQFDQLKK", "aa_hexdigest": "a594f942a7a2dd69ad44d3979adbeac2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16442.641599999984, "isoelectric_point": 8.889432716369633 }, "id": "EFPKPENHBP_422", "locus": "GCA000186005_00387" }, { "type": "cds", "sequence": "contig_1", "start": 206429, "stop": 206923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGTTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGA", "aa": "MLCKLDLNLQTAKKIAELHQSLINEYKQKRLELETIERKIELENFKILYMTQTANFADPVVIEQYNRILHKFEENINTHMPTYIAKVVDEQFKAADLKGKQVLEILKNPTTYNQVSIFISEIEKTNRYFKDFKNKVLLSQAELVAKIQVEVNSDENLYNLNKKF", "aa_hexdigest": "540e9ee28e22a1aa6a8b723a4de1ee78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19371.179500000002, "isoelectric_point": 7.757180976867676 }, "id": "EFPKPENHBP_423", "locus": "GCA000186005_00388" }, { "type": "cds", "sequence": "contig_1", "start": 206987, "stop": 207409, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGA", "aa": "MTYEIEDNNNKKISVTKKAKERMFDSYAQEEDELNQVATHLTNRISTHQTVLDNINAKINQLQVEYKDPKITAERKVEIIELLKQIAKLYDNTKTAIYHINDVLREIKLAISNLLEIKQGITNGTHTYLDAYDLDSNLLS", "aa_hexdigest": "80eaa76e9341eaea77431378e92d201e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16194.129399999987, "isoelectric_point": 5.568140983581542 }, "id": "EFPKPENHBP_424", "locus": "GCA000186005_00389" }, { "type": "cds", "sequence": "contig_1", "start": 207647, "stop": 208189, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTTTAGCTAAAAATTCAGAAATTGGTAATATGATTCTCGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGA", "aa": "MDVANKIETSLYKEAINKEIESTLAYKASENSIKNQIEFFSNLMPDYSKIINGLAKNSEIGNMILGLNIEVEKSCANDPLIKKLNNVLKDLDDPNSEINKFKEKAQNAYKTAGLKDQLDNIESEKSKYGKMTDSFIKLLNDTIKVKILSNYIKNESIDIESYVKNTNNEARLADTKDAVT", "aa_hexdigest": "5e3c6fe1d93397057903f4bfbcf77360", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20293.77760000001, "isoelectric_point": 5.243760871887206 }, "id": "EFPKPENHBP_425", "locus": "GCA000186005_00390" }, { "type": "cds", "sequence": "contig_1", "start": 208430, "stop": 208675, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAGCTCATTTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGA", "aa": "MIKAHLEIIDKSRIEAIEKSMPKLNQDEKLVKYEAIINEGFQKTIDKLYNIDENLKTINLFDNAFERMAISKDFIETRTYD", "aa_hexdigest": "2b867cdb640047d6a05a2b41a46e878e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.920599999992, "isoelectric_point": 5.165266227722166 }, "id": "EFPKPENHBP_426", "locus": "GCA000186005_00391" }, { "type": "cds", "sequence": "contig_1", "start": 209105, "stop": 209830, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGA", "aa": "MFNKAVDDHMKQTEKILKYQAPISNFFNEFNREYLTLLNPNIVAFEKIANSKIASIKTAIGELINARIREILISGREFPLYDSKNNGFEYKDADSIIVAKSRSELIDILSKKKLVNANDSVEKINKDIYFVRLSHLKKDGTKLVVTLRNILTDEKEMFANGYSDKYIKFNIDASVNDKRLSTLNALFTAANYKKQVTPTLIREEGNIRDENGKSVKGYSVFVDAYQDMTEKLLHEVPYAGE", "aa_hexdigest": "333bc44d9304527628d94babca1320b2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27578.191400000025, "isoelectric_point": 8.748891639709473 }, "id": "EFPKPENHBP_427", "locus": "GCA000186005_00392" }, { "type": "cds", "sequence": "contig_1", "start": 209954, "stop": 210319, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGA", "aa": "MTDPNFKGISTDFLKFVGAHEYGHHITLNGAHDLGNKGNNPIYVSALTPGATPHIDNYYSKDALELYLKARTHLELNTTRLLKENNVVIDYGEYITFGIPKIEIVNGKPNLIYKNENEENI", "aa_hexdigest": "e3d3f63e6fd1a4e7ad53e1f909ebee3d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13631.155199999985, "isoelectric_point": 5.810559272766113 }, "id": "EFPKPENHBP_428", "locus": "GCA000186005_00393" }, { "type": "cds", "sequence": "contig_1", "start": 210380, "stop": 210559, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGACGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGA", "aa": "MQNFKGLLEAVIARRQANGLDDDYEKYLTAFDLWLMNSLDHHSGTINPSLRGGTAKYMV", "aa_hexdigest": "40be366d4999376e777c12fbbec82708", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6687.509600000001, "isoelectric_point": 5.999548912048339 }, "id": "EFPKPENHBP_429", "locus": "GCA000186005_00394" }, { "type": "cds", "sequence": "contig_1", "start": 210740, "stop": 211003, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGA", "aa": "MFNEDGTPVINVPLHVDFNDKSSPYYDANAIKYVNSKIKAIIETIKSLIVEKYSINGWDTTATDLSLEPKFILSNLYLSTLLGKNDD", "aa_hexdigest": "40c2b22ba963ea8f116b9e1fcfc22e45", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9784.96219999999, "isoelectric_point": 4.748409080505371 }, "id": "EFPKPENHBP_430", "locus": "GCA000186005_00395" }, { "type": "cds", "sequence": "contig_1", "start": 211070, "stop": 211414, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGA", "aa": "MIDDSKPLPYFDNSGKLIYPSNKKIPQASIYANIKKYALGIKRPEDLTSLMFAMNTAGDSFNNVVNSGEGHILYVDKDHQYLPNINLAQTFENNFLYNYVADMADVLQLKKIMK", "aa_hexdigest": "fc7fa4955eac2188585ff404244219ba", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12968.71679999999, "isoelectric_point": 6.711570930480956 }, "id": "EFPKPENHBP_431", "locus": "GCA000186005_00396" }, { "type": "cds", "sequence": "contig_1", "start": 211541, "stop": 211732, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGA", "aa": "MSINEKIIEKDPVLSLFNAFYSIEGSQKLFGKDVAFTDYKKFLEFVSVDLKQAKLDKNTKVVN", "aa_hexdigest": "57091bbb1cd30df09ed7768297cc6004", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.293100000003, "isoelectric_point": 7.942972373962403 }, "id": "EFPKPENHBP_432", "locus": "GCA000186005_00397" }, { "type": "cds", "sequence": "contig_1", "start": 212195, "stop": 212497, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGA", "aa": "MLESKNSVYGLLLTLGKGIFKKAKPSQDVLDYYGSKTERKFNEFFTDYTYNFAEVINRDNLQITYSPSNSNFGNMPSYLSNLSESNTGLEYVVDGAATSR", "aa_hexdigest": "95284cf6269465cfaa8a247f74256388", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.379299999991, "isoelectric_point": 5.302987098693847 }, "id": "EFPKPENHBP_433", "locus": "GCA000186005_00398" }, { "type": "cds", "sequence": "contig_1", "start": 212573, "stop": 212719, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGA", "aa": "MLNEEARRRSQILNTRFIERVFANQDNMQYGQNYKNNYFGQFKTINNG", "aa_hexdigest": "1dfe9e9dded48c15f5990b26ac6cfcd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.407800000001, "isoelectric_point": 9.812748908996582 }, "id": "EFPKPENHBP_434", "locus": "GCA000186005_00399" }, { "type": "cds", "sequence": "contig_1", "start": 212936, "stop": 213190, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGA", "aa": "MFGYLSTAIADKANYLAFKNVRTGEVKTLRITKQNASNMFYYKEQQVNNEIRHQNGEKVRHYLIDEPFDFTDKNGHQKGTGFVA", "aa_hexdigest": "87fccb8041248ebca426ed11b6fc2cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9747.825799999997, "isoelectric_point": 9.259610176086426 }, "id": "EFPKPENHBP_435", "locus": "GCA000186005_00400" }, { "type": "cds", "sequence": "contig_1", "start": 213209, "stop": 213316, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGA", "aa": "MSRYRNRLLDTNEEYYVYFASDEQGNKALEADLGT", "aa_hexdigest": "45c3ce5d1dbd9e45a270f0d42f4d2ea9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4133.422600000001, "isoelectric_point": 4.440682792663575 }, "id": "EFPKPENHBP_436", "locus": "GCA000186005_00401" }, { "type": "cds", "sequence": "contig_1", "start": 213463, "stop": 213948, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGCGAAAGTAGATGA", "aa": "MKRNKLILSLASLSTISTASIVAVSCGNKAYKTNYDLGLVTEPINSLNYLKFNSVSKVLPSIVESPLKTGPNEALKRILSLPEIPMGVYGNDDKSNSMDDYIANNRAPKEASGRFYPLDQFGSAPGTINTDRSEYQPVSVVMTKNNKILSLHITLNRGESR", "aa_hexdigest": "00fcbe3ecaa3d9c41544572d195dd2dd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17610.908699999993, "isoelectric_point": 9.324529838562015 }, "id": "EFPKPENHBP_437", "locus": "GCA000186005_00402" }, { "type": "cds", "sequence": "contig_1", "start": 213991, "stop": 214167, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGA", "aa": "MHYILDINTGSQKQTNILQRKFRSSSSLIDAQQEYIKKHKKAYSNPFAYPKLIKENGQ", "aa_hexdigest": "c4826ade9e167ede28aa5312607bf347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6799.682800000002, "isoelectric_point": 9.85201015472412 }, "id": "EFPKPENHBP_438", "locus": "GCA000186005_00403" }, { "type": "cds", "sequence": "contig_1", "start": 214378, "stop": 215061, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTAGTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGA", "aa": "MLPNPEYSLSRHTAEELKLIPQRIATKIRKYLYFDPRQSYSETKFKPLVKKAKKLKSRMNKNVSFEKDINEYNQEVNKLYGKENTLNNNSIDSREFMENRVLALDEFSLRVEYDSNEPTSLSNAYSDIQSTLIPVNRKFVESIGGIREFGLDKSKFLTNGPFYIKNIVLGPQGYMELVKNNTYYSSTKTISDSIKIYFSSDANINSAMYDDGYIAATKIPAVQQINY", "aa_hexdigest": "1897cee0dcccce0a62f0b090b7ea841e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26218.381600000015, "isoelectric_point": 9.070331001281737 }, "id": "EFPKPENHBP_439", "locus": "GCA000186005_00404" }, { "type": "cds", "sequence": "contig_1", "start": 215086, "stop": 215235, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGA", "aa": "MKKSSGFGTIALAFNLDQETNKNSLINDVDLRNALYFGINRNEMLNIVG", "aa_hexdigest": "3d45a261e11820698e48f301f580578c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5461.146999999999, "isoelectric_point": 6.0008562088012685 }, "id": "EFPKPENHBP_440", "locus": "GCA000186005_00405" }, { "type": "cds", "sequence": "contig_1", "start": 215329, "stop": 215796, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGA", "aa": "MKTKVSDKKIPIQNYNHVDHLAKQYNNEHVDRTDLGYNLEIARAYMQRFKDKHPDVKQVTLKYISNSTDEQQNAGIALKDFIKKAFGDYLIIDVQGLPENVYEDFRTTGKYDLIYRNFDTFGSDSYSYVKVFFKPDEIDRKNQKTTGFRNNPSGS", "aa_hexdigest": "10a1e4ba01d5dafbc5d51422879e05b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18135.033199999983, "isoelectric_point": 8.583530235290528 }, "id": "EFPKPENHBP_441", "locus": "GCA000186005_00406" }, { "type": "cds", "sequence": "contig_1", "start": 216283, "stop": 216396, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGA", "aa": "MQKRNDQNYFTGTVNKDQYNLYNREVDFSGKAKKDNR", "aa_hexdigest": "4d5062402735ed610fcae5a083d11ceb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.849100000002, "isoelectric_point": 9.520900535583493 }, "id": "EFPKPENHBP_442", "locus": "GCA000186005_00407" }, { "type": "cds", "sequence": "contig_1", "start": 216730, "stop": 217089, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCGAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGA", "aa": "MIGIFSVFLTLIIGIPVGVYVGMNPDKLPDHIATLIVSVFSSIPSLVFALLLLLIGRSLNMPYLFNELDIATYVLPGLALSLGSIIVYIKYIRTELNRELNSQHAKFCYLKGLSKRRFV", "aa_hexdigest": "b5f7ee1c8f44c5e7baa8aa8fe17ce081", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13272.822699999979, "isoelectric_point": 9.41491451263428 }, "id": "EFPKPENHBP_443", "locus": "GCA000186005_00408" }, { "type": "cds", "sequence": "contig_1", "start": 217168, "stop": 217350, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAG", "aa": "MFVEQIFFITGSGGLLIQAITSKDYNIILFMVTLFSLITILSYACRDALYQIIDPRVRRR", "aa_hexdigest": "a44c8e6b125770220820a8f7800758e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6946.2058, "isoelectric_point": 9.098052406311037 }, "id": "EFPKPENHBP_444", "locus": "GCA000186005_00409" }, { "type": "cds", "sequence": "contig_1", "start": 217525, "stop": 217746, "strand": "+", "frame": 1, "gene": null, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "nt": "ATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGA", "aa": "MHKQKKPFLEFFYRFSRSTAGVFGFVALALLIILAIFLPFTTKDPNTVNTEKRYLIFFTDGHIFGTDALGRDI", "aa_hexdigest": "76472b4940abb58951f87b0170df7f8a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAE2EIN1", "query_cov": 1.0, "subject_cov": 0.9733333333333334, "identity": 0.6579999999999999, "score": 102.0, "evalue": 2.4e-27, "valid": false, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "uniref50_id": "UniRef50_A0AAE2EIN1" }, "pscc": { "uniref50_id": "UniRef50_A0AAE2EIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "product": "N-terminal TM domain of oligopeptide transport permease C family protein" }, "genes": [], "id": "EFPKPENHBP_445", "locus": "GCA000186005_00410" }, { "type": "cds", "sequence": "contig_1", "start": 218137, "stop": 218361, "strand": "+", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "nt": "GTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAA", "aa": "MFHIPGVILSETSLAFIGLSITDIPTLGNLINDGSKIFTTFPRYVLIPSTMLIIVTTSIQLMSAAIQDSLLRQR", "aa_hexdigest": "ff062bc2a790fe596087ebfb724b0aab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGD7", "query_cov": 1.0, "subject_cov": 0.8809523809523809, "identity": 0.662, "score": 100.0, "evalue": 2.64e-26, "valid": false, "uniref50_id": "UniRef50_A0A3B0PSU9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PSU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "product": "ABC transmembrane type-1 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_446", "locus": "GCA000186005_00411" }, { "type": "cds", "sequence": "contig_1", "start": 218654, "stop": 219421, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3", "UniRef:UniRef90_A0A3P8MDR3" ], "nt": "GTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAACCTAAAATTTTAGTCATGGATGAGCCCACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCAATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGA", "aa": "MAAEIEDVYLTFRNPADPKEKNLVLRGPSLKIYEGKVHAIIGESGSGKSVITSLLYGLTGSNAIIESGKIKLYGLEVQDYTMYNWEKSKLRGRVVSAVFQNPMSILDPTMKVGKQIIEGMLINKIVKTKKEAKAEAIKYLEMTKINNPQKVMDLYPHELSGGMIQRIAIAAIVSLKPKILVMDEPTTALDPTVQALVLDIIRELQERFHIAIAFITHDLGVVASISDFINIMYAGQIIESGTKEEILQNPQHPYT", "aa_hexdigest": "fccfa6d2da0b76016ecd842b85b4407c", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR3", "query_cov": 0.8117647058823529, "subject_cov": 1.0, "identity": 0.9520000000000001, "score": 376.0, "evalue": 1.63e-130, "valid": true, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MDR3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_447", "locus": "GCA000186005_00412" }, { "type": "cds", "sequence": "contig_1", "start": 219437, "stop": 219670, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07", "UniRef:UniRef90_A0A3P8LB07" ], "nt": "ATGCCTGACTTTAATAAAGGTACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCAGTTAGAAATGACTATGCTTTAGGAATAGATTTCGAAGAAGAATCACCTTTCTATCAAATCTCTCCTACACACTTTGTTAAAAGTAATTTGCTTAATGGACAAGCCCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGA", "aa": "MPDFNKGTKLQVIRGAVPSNLNNIDGDAFAVRNDYALGIDFEEESPFYQISPTHFVKSNLLNGQAPKYEPPKIIQNL", "aa_hexdigest": "6d3971f89d03edccc517277886356ddc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 219437, "stop": 219862, "observations": { "mutation": [ 219668 ], "stop": [ 219668 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LB07", "query_cov": 1.0, "subject_cov": 0.5460992907801419, "identity": 0.857, "score": 135.0, "evalue": 2.2e-39, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MPDFNKDSKLQVIRGAVPSNLNNIKGDAFAVRNDYALGIDFEEESPYYQISTTHFVKSNLLNKNVSKYEPPKIIKRLWDKYENQMNSIYGDDKYVSQKVYDEFVQKAYENNELVKKLIAKREEEKAIKQSTEVKKPGEKNA" }, "paralog": false, "identity": 0.723404255319149, "score": 206.0, "evalue": 1.32e-69, "description": "Internal stop codon at 219668. Nonsense mutation around 219668.", "psc": { "uniref90_id": "UniRef90_A0A3P8LB07", "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LB07", "UniRef:UniRef50_A0A3P8LB07" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8607.587299999994, "isoelectric_point": 5.115418434143066 }, "id": "EFPKPENHBP_448", "locus": "GCA000186005_00413" }, { "type": "cds", "sequence": "contig_1", "start": 219855, "stop": 220196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGTACAGATGAAATGCTTAATGCATATCCAAATAAAAAAGCTTTAGTAGAAATAAGAAATTTAGATATTGATTATGGTTTTGGAGCTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGCGAAGTTTTAGGACTTGTTGGTGAAAGTGGCAGTGGTAAAAGTACCACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGA", "aa": "MPKYYYEKRKFGKPLAREIRQGTDEMLNAYPNKKALVEIRNLDIDYGFGAKKFSAIKDLNLNIYDGEVLGLVGESGSGKSTTGRAIIGLTPHTYGYIKILDRIIPKNLDKGFK", "aa_hexdigest": "377bf9bd8060109b3c9803d3fbd39afc", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 46, "aa_cov": 0.40707964601769914, "hmm_cov": 1.0, "evalue": 5.270981577241561e-12, "score": 42.85324478149414, "start": 57, "stop": 102 } ], "seq_stats": { "molecular_weight": 12730.593599999986, "isoelectric_point": 9.572088432312011 }, "id": "EFPKPENHBP_449", "locus": "GCA000186005_00414" }, { "type": "cds", "sequence": "contig_1", "start": 220227, "stop": 221093, "strand": "+", "frame": 3, "gene": null, "product": "Oligopeptide ABC transporter, ATP-binding component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "nt": "ATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAATTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACACGCTTTGATCAAATAGTTTTAAAAGAAGTAAATAGATTGATAAATAAAATTGATTCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATAACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAGATAATTTAAGTGAAAAAGAATGTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTGTACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATCGCAGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAAGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCAGATAGAATAGCTGTTATTAATAAAGGTGTCTTACTTGAAGTAGGTCCAACTGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCCGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGA", "aa": "MVNKVQMIFQDPTNSLNPYKNVETVVGEGLTNLKNSKYIFLTRFDQIVLKEVNRLINKIDSTNSLISNLLEKCREIEVSIEKSYNFVFKELIDEINRRYTTNANYKDALEYLESQKVERDSLDNLSEKECKKRLIIDILKQVGLDETVLGRFPLEFSGGQQQRLGICRSVVLQPKLLIADEPISALDVSIQAQVINIFNELKEKYNLTILFIAHDLRMVEYISDRIAVINKGVLLEVGPTDEIIHNAHHPYTRSLLDAVPSIESEKGSLLGYIYDPSMHKYDENNQPV", "aa_hexdigest": "acebac3e5e9c77a1189a8b17d93a7de6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q6MRZ9", "query_cov": 1.0034722222222223, "subject_cov": 0.903125, "identity": 0.6509999999999999, "score": 359.0, "evalue": 1.07e-121, "valid": false, "product": "Oligopeptide ABC transporter, ATP-binding component", "uniref50_id": "UniRef50_Q6MRZ9" }, "pscc": { "uniref50_id": "UniRef50_Q6MRZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "product": "Oligopeptide ABC transporter, ATP-binding component" }, "genes": [], "id": "EFPKPENHBP_450", "locus": "GCA000186005_00415" }, { "type": "cds", "sequence": "contig_1", "start": 221118, "stop": 221843, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCTACCGATGAAGAATTAGCTAAATACAAAAGAGATAGAACTAGACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTGAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGAAGATGATGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAAACATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAAATAGTAGAAAAACTTGCTAAAGAAAATGAATAA", "aa": "MLATDEELAKYKRDRTRLNEIEAKRERNEKITLDDFIIEENVINEQEKYDLVTKIKEAQIDLNNYNEGSEVKITPSYVKTATVIQEEVSTYDSLKETTKLIKEDDLPEVKVTPSYIEVAKVIEDDEIKKTEKSSTKKVEKITIISNVHKDSTVELIPENDKITVEEKPKKVRKTRKVSATLKKPTKKSTTKKQTKTSTITVDLSKKIKPNKGVTKAELRAQARKEKSANKKIVEKLAKENE", "aa_hexdigest": "8331c57e384b9c4550972a514e6a31a5", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27677.369600000064, "isoelectric_point": 9.201137351989747 }, "id": "EFPKPENHBP_451", "locus": "GCA000186005_00416" }, { "type": "cds", "sequence": "contig_1", "start": 221880, "stop": 222284, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354444.1", "SO:0001217", "UniParc:UPI0001A8C120", "UniRef:UniRef100_C4XEF3", "UniRef:UniRef50_C4XEF3", "UniRef:UniRef90_C4XEF3" ], "nt": "ATGGATAAGGCTAAGAAAAAGAAAATTTTAATTCCACTCTTAAGTACTGTTGGTGTTTTAGCTGTTGTTACTCCAATTGTTGTTGTATCTGCAGCTAAAAGTAAACATAAGGAAATTGAAAAACAAGAAACAATAAATTCTTTTGAAGAATTTCCTAAATTAGAATATAACAACTATTACAAGTACATTAGAATAGGTGAAAAAGGCGAAGAATATATTGATGAAAATGTTGTGCCTTTAATAGTGAAAGATGTTTTTCAAAAACTAAACGCAGATTATCAAAGTAAAATTTCTTTTGATTATCAATTTGTAAGCAAAAATCATTTAGAACTTAAATTTAAATATGTTTCAGAAGACAAAACACTTTTCAAAACTTATGTTTTAAAAACTAAAAATATGGTTTAA", "aa": "MDKAKKKKILIPLLSTVGVLAVVTPIVVVSAAKSKHKEIEKQETINSFEEFPKLEYNNYYKYIRIGEKGEEYIDENVVPLIVKDVFQKLNADYQSKISFDYQFVSKNHLELKFKYVSEDKTLFKTYVLKTKNMV", "aa_hexdigest": "b103e33d00b36b030ab5faad621b356a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C120", "ncbi_nrp_id": "WP_013354444.1", "uniref100_id": "UniRef100_C4XEF3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C120", "RefSeq:WP_013354444.1", "UniRef:UniRef100_C4XEF3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEF3", "uniref90_id": "UniRef90_C4XEF3" }, "psc": { "uniref90_id": "UniRef90_C4XEF3", "uniref50_id": "UniRef50_C4XEF3" }, "pscc": { "uniref50_id": "UniRef50_C4XEF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15662.085599999993, "isoelectric_point": 9.171546363830569 }, "id": "EFPKPENHBP_452", "locus": "GCA000186005_00417" }, { "type": "cds", "sequence": "contig_1", "start": 223147, "stop": 223614, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGATATTTCGCAAGAACGTTATGTATACAAATCAGAATATTTAGATGCTCCATCTTATTTAACTAAAGATCACTATGATGTAAATTTTAAAGATGCATGCAATATTGTAAATGGTAATATTGAAATAAAAAGATATTTTACAGAAGATAGATTTGCAATTAAAATATTTTTTATTCAACCCAAAAATTTACATAAAAAATTCTTTGAAGGTTTTGATATTGAAATAAATGGAAAAAGAATTGATGGAAACATAATTAGAAGCTGCACAAAATTGGAAACAATGGATAGCAGTTTACTAAATGAAAGACTTAGTGTTAAAAAATGGTATTCGAAATTTACTGAAGAAACAAGTGCATATGGATTAACAATCCTTTCACCTAAAATTATAAATTTACCTTACGAAAAATTCCGTATTTTAAGAGTTTACTTTAGAAGTAAAGAAAAAACAGAAAAACAATATTGA", "aa": "MHDISQERYVYKSEYLDAPSYLTKDHYDVNFKDACNIVNGNIEIKRYFTEDRFAIKIFFIQPKNLHKKFFEGFDIEINGKRIDGNIIRSCTKLETMDSSLLNERLSVKKWYSKFTEETSAYGLTILSPKIINLPYEKFRILRVYFRSKEKTEKQY", "aa_hexdigest": "9adade7555a4b8bb602a84ccaa94efc4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18612.110800000002, "isoelectric_point": 9.052537727355958 }, "id": "EFPKPENHBP_453", "locus": "GCA000186005_00418" }, { "type": "cds", "sequence": "contig_1", "start": 223727, "stop": 223984, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5", "UniRef:UniRef90_C4XEF5" ], "nt": "ATGAATCTAACTTTTAATGATTATTTTATGGGCTTAATTTCACATAAAGATCAAAATAGTGTTTTACACAATATCTTTAAAATGGAAAAAGTCAATGAGCAAGCTTACAAAAAGACAATTGGAGGAGGCAACAAATCAAACATACTTAAAAACATCTTTAAACCAAAAAATAAAAGTCAGCATATTTTATCTATTATGAAACCTGAACTTGCGCAAATTATCAAAGAGGATTTCTTAAAAAGTCAATCTAAAAATTGA", "aa": "MNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIGGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKN", "aa_hexdigest": "622a308b90e7224fe28346681ade097f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 223640, "stop": 223999, "observations": { "mutation": [ 223729 ], "stop": [ 223729 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF5", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 1.0, "score": 168.0, "evalue": 1.75e-52, "gene_start": 1, "gene_end": 85, "reference_start": 6, "reference_end": 90, "reference_sequence": "MERRKMNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIGGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKNWFKDYYSKNTYYKYKKQAVEEFLYHYFNE" }, "paralog": false, "identity": 0.9831932773109243, "score": 240.0, "evalue": 1.9e-83, "description": "Internal stop codon at 223729. Nonsense mutation around 223729.", "psc": { "uniref90_id": "UniRef90_C4XEF5", "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF5", "UniRef:UniRef50_C4XEF5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9839.358899999996, "isoelectric_point": 9.778387260437011 }, "id": "EFPKPENHBP_454", "locus": "GCA000186005_00419" }, { "type": "cds", "sequence": "contig_1", "start": 224167, "stop": 224367, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAGAGTTGATAAAGATTCCTTATTAAAACAAGCTGAATTTAGTCATGTTTCTCAAGATGAATTTAAAGACTATCATAAAAATATTATTAATAATACTTTCCCTTTAACTATTGGCGGTGGTATAGGGCAAAGTAGACTTTCAATGTTCTTGTTAGAGAAGAAACACATTGGTGAAGTTCAAGTAGGTATTTGA", "aa": "MGIRVDKDSLLKQAEFSHVSQDEFKDYHKNIINNTFPLTIGGGIGQSRLSMFLLEKKHIGEVQVGI", "aa_hexdigest": "28e07f933ce3c23cef55071f2989b38e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.448299999997, "isoelectric_point": 6.820531272888183 }, "id": "EFPKPENHBP_455", "locus": "GCA000186005_00420" }, { "type": "cds", "sequence": "contig_1", "start": 224431, "stop": 224700, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAAAAAAATAGTCAACACAATTTTTAAATGTATTCGTCATGTTGAATACAAACTTTTAGTTGAATTTCCACAACTAAGTCAAAAATTACCTGAAAATGTAACTTTTATTAGTAGTAGTGAGCTTTACAACTTATATCCAAAATTAAGCCCTGAAGAAAGAGAAACAAAATTTGCTAAAAAACACAAGGCTATATTTATTTATGAAATTGGTTATCCTTTAGCAGATAATAAGCCTCAATCAAAACGTGCTTTTGACTACGATGACTGA", "aa": "MKKIVNTIFKCIRHVEYKLLVEFPQLSQKLPENVTFISSSELYNLYPKLSPEERETKFAKKHKAIFIYEIGYPLADNKPQSKRAFDYDD", "aa_hexdigest": "94c5f14c16e70b051e30b92ae4251115", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 89, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.5434444266474577e-29, "score": 99.6573486328125, "start": 1, "stop": 89 } ], "seq_stats": { "molecular_weight": 10573.116699999986, "isoelectric_point": 8.967632865905763 }, "id": "EFPKPENHBP_456", "locus": "GCA000186005_00421" }, { "type": "cds", "sequence": "contig_1", "start": 224866, "stop": 225096, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGTACAAGAGTAAATTAAACATTAAAGAAACTCAATTAGCAATTCAAGATTTGAAGTTTGCATTTACTAAAAAATTAAACAAGGAATTACATTTAACTAGAGTTTCAGCCCCTTTATTTGTTGCTAGAAATACCAAAATAAATGATGGCTTAAATGGGGAAGAACCTGTATCTTTTGTTCCTAAAAATTTTGATGAAACTGTTGAAATAGTTCACTCTCTTGCTAAGTGA", "aa": "MYKSKLNIKETQLAIQDLKFAFTKKLNKELHLTRVSAPLFVARNTKINDGLNGEEPVSFVPKNFDETVEIVHSLAK", "aa_hexdigest": "fd57b4d2e512df91a6ada4ac12db9f79", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 2.792227604539184e-19, "score": 66.10094451904297, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8657.9479, "isoelectric_point": 9.347029304504396 }, "id": "EFPKPENHBP_457", "locus": "GCA000186005_00422" }, { "type": "cds", "sequence": "contig_1", "start": 225083, "stop": 225244, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCATTGTTAATTTACATATCACAATTATCAATGATGTTACACATCTTGAAAACAACAAAAAAAGCTATGCAAGAAGCTAAAAATAATCAAGAAAATTCAATTCCACAAAATGACCAAAACAACTATAAAACAGAGGCTAACGAAAATGTACAAGAGTAA", "aa": "MSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE", "aa_hexdigest": "d104dafb46ec731f98f5447d9804ae5b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6168.919100000002, "isoelectric_point": 5.697790718078613 }, "id": "EFPKPENHBP_458", "locus": "GCA000186005_00423" }, { "type": "cds", "sequence": "contig_1", "start": 225323, "stop": 225682, "strand": "-", "frame": 3, "gene": null, "product": "PQ loop repeat", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7", "UniRef:UniRef90_C4XEF7" ], "nt": "ATGGCGGCTGTAGTTTATGCCAATATTTTGTGTGCTTATGTCTATTGTGTACTTTTATTCTTTATGTACAAATATGATGAAAAAGTAAAAAGAAACAAAAGAAAATTTGTAGTTTTAGGAATAACATTAACTTTAACTACAATTTGTGCAATTGTTGGTTTTGTCGGTTTATATCACAAAAACAGTGAAGGCAATGGTCTTAATTTTGATCCTAATACAACTGCTATTTTGTCTCAAATTATTCCAGTTATATCAACTTTTGCATTCTTACCACAAGTATTAAAAACATTTGAAAAGAGAGATATTGAAGGCTTATCAGTTGGTTTAATTTTAATGTTTGTATTAACAAATATTTTTTGA", "aa": "MAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIF", "aa_hexdigest": "c7f5c9bfba956729557ae0903bac8929", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 225083, "stop": 225922, "observations": { "mutation": [ 225253, 225313, 225325, 225721, 225748 ], "stop": [ 225253, 225313, 225325, 225721, 225748 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF7", "query_cov": 1.0, "subject_cov": 0.4265232974910394, "identity": 1.0, "score": 233.0, "evalue": 1.6e-75, "gene_start": 1, "gene_end": 119, "reference_start": 81, "reference_end": 199, "reference_sequence": "MDFIRSSFAINKDVNDGIAAFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSFWIFFAALCSWIIFGSFTGEEAKMAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIFWIAYWVSLIIAEGKLTPALTSTLLWQTLSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE" }, "paralog": false, "identity": 0.982078853046595, "score": 523.0, "evalue": 3.94e-192, "description": "Internal stop codon at 225253, 225313, 225325, 225721, 225748. Nonsense mutation around 225253, 225313, 225325, 225721, 225748.", "psc": { "uniref90_id": "UniRef90_C4XEF7", "product": "PQ loop repeat", "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF7", "UniRef:UniRef50_C4XEF7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7" ], "product": "PQ loop repeat" } }, "id": "EFPKPENHBP_459", "locus": "GCA000186005_00424" }, { "type": "cds", "sequence": "contig_1", "start": 225746, "stop": 225922, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTATACGTAGTTCTTTTGCTATAAATAAAGATGTTAATGATGGCATAGCAGCGTTTGTAGCAATAGTAGGAATGATAGCATGTATTGCAACAATAAGTGTATCAATTCCTCAATTAGTTTATTTATTAAAAAGAAAGAAAACTGGGGAGGTTAAATTCTATTCATTCTGA", "aa": "MDFIRSSFAINKDVNDGIAAFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSF", "aa_hexdigest": "996b09043a343c4a26662b57c1bf715b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6401.559499999999, "isoelectric_point": 9.35966510772705 }, "id": "EFPKPENHBP_460", "locus": "GCA000186005_00425" }, { "type": "cds", "sequence": "contig_1", "start": 226163, "stop": 226588, "strand": "+", "frame": 2, "gene": null, "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526740.1", "SO:0001217", "UniParc:UPI0001A8C125", "UniRef:UniRef100_C4XEF8", "UniRef:UniRef50_C4XEF8", "UniRef:UniRef90_C4XEF8" ], "nt": "ATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTTTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAAATTAAAGCTTTTTATTATTATATGGATTGCGGATATAGTCCTTGCAAACCAAATAAAGAATATGTTGAAATGTGCTTAAAAGGATACCAAGACTGCAGTATTAAAAAAGAACAACTTAGAGAGATTTTATACAAGTAA", "aa": "MKKYYIAYGSNLNSGHMENLCPSAKFLGKVILNNFSLSFEGEKDRCYLNIRKVKDKNIEVGVFELNDKDIFKLDEYEDYPFLYKKEDIEIELNSKKIKAFYYYMDCGYSPCKPNKEYVEMCLKGYQDCSIKKEQLREILYK", "aa_hexdigest": "5208f99e708b19b80dd149db43d84edf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C125", "ncbi_nrp_id": "WP_013526740.1", "uniref100_id": "UniRef100_C4XEF8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C125", "RefSeq:WP_013526740.1", "UniRef:UniRef100_C4XEF8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEF8", "uniref90_id": "UniRef90_C4XEF8" }, "psc": { "uniref90_id": "UniRef90_C4XEF8", "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "uniref50_id": "UniRef50_C4XEF8" }, "pscc": { "uniref50_id": "UniRef50_C4XEF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF8" ], "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_461", "locus": "GCA000186005_00426" }, { "type": "cds", "sequence": "contig_1", "start": 226626, "stop": 226889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A111", "UniRef:UniRef100_A0AB32XB79", "UniRef:UniRef50_A0AB32XB79", "UniRef:UniRef90_A0AB32XB79" ], "nt": "ATGATTAACAAGGAATATAAGCCTATATTCAAGGTTACAAACAAAATAATTGAATTAATTGGCAAGATTAAAGAGAAATTGTCAAGCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAAGGTGCTGTAA", "aa": "MINKEYKPIFKVTNKIIELIGKIKEKLSSVFLCKDFLILNRKIKIKSIYSSVKIEGNPYDFQTFYKVINNEEKEQKDKYKLEFKKVL", "aa_hexdigest": "519bc18f3343baa767c8cdbcd657e2a3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A111", "uniref100_id": "UniRef100_A0AB32XB79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A111", "UniRef:UniRef100_A0AB32XB79" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB79", "uniref90_id": "UniRef90_A0AB32XB79" }, "psc": { "uniref90_id": "UniRef90_A0AB32XB79", "uniref50_id": "UniRef50_A0AB32XB79" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XB79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XB79" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10453.347299999992, "isoelectric_point": 9.630948066711426 }, "id": "EFPKPENHBP_462", "locus": "GCA000186005_00427" }, { "type": "cds", "sequence": "contig_1", "start": 226984, "stop": 227190, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 226984, "stop": 227293, "observations": { "insertion": [ 227263 ], "mutation": [ 227188 ], "stop": [ 227188 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9901960784313726, "score": 192.0, "evalue": 5.56e-65, "description": "Internal stop codon at 227188. Frameshift due to insertion around 227263. Nonsense mutation around 227188.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "EFPKPENHBP_463", "locus": "GCA000186005_00428" }, { "type": "cds", "sequence": "contig_1", "start": 227254, "stop": 227478, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDELVSMNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "ee1a46cfc99fa70b8a20f44b791b3aad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8704.383199999993, "isoelectric_point": 10.111495399475096 }, "id": "EFPKPENHBP_464", "locus": "GCA000186005_00429" }, { "type": "cds", "sequence": "contig_1", "start": 227590, "stop": 227862, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTDNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "a6cf9241f23083862b43c93386ae60e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10698.441299999997, "isoelectric_point": 9.751826286315914 }, "id": "EFPKPENHBP_465", "locus": "GCA000186005_00430" }, { "type": "cds", "sequence": "contig_1", "start": 228701, "stop": 229201, "strand": "+", "frame": 2, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETELKRALTNDATSKLIKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYEKFIQYCAKRLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "db6b55899c8f181b6b7105e638679bda", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 228701, "stop": 229504, "observations": { "mutation": [ 229199, 229256, 229322, 229436, 229448 ], "stop": [ 229199, 229256, 229322, 229436, 229448 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9520000000000001, "score": 316.0, "evalue": 3.11e-107, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.947565543071161, "score": 503.0, "evalue": 2.02e-183, "description": "Internal stop codon at 229199, 229256, 229322, 229436, 229448. Nonsense mutation around 229199, 229256, 229322, 229436, 229448.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "EFPKPENHBP_466", "locus": "GCA000186005_00431" }, { "type": "cds", "sequence": "contig_1", "start": 230308, "stop": 230778, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526745.1", "SO:0001217", "UniParc:UPI0001F5A115", "UniRef:UniRef100_C4XEG3", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAGTATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESISKLIDSKTIINFDIAKLQSINKYFGFGISDINLLKINQVLFDYELLKTLRLENNLLQLNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEEKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "915f0a1a2ff5d36ed2d11f97c42870a7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A115", "ncbi_nrp_id": "WP_013526745.1", "uniref100_id": "UniRef100_C4XEG3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A115", "RefSeq:WP_013526745.1", "UniRef:UniRef100_C4XEG3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG3", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18867.681699999994, "isoelectric_point": 8.92366542816162 }, "id": "EFPKPENHBP_467", "locus": "GCA000186005_00432" }, { "type": "cds", "sequence": "contig_1", "start": 230792, "stop": 230956, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGTATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESDNVGYYVTFPDNKSIKLNQELKMCLYEIKNDFT", "aa_hexdigest": "d4f2fe22c90eab35e78a6f8e6cf0c5a3", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6428.4094000000005, "isoelectric_point": 8.732967948913572 }, "id": "EFPKPENHBP_468", "locus": "GCA000186005_00433" }, { "type": "cds", "sequence": "contig_1", "start": 230908, "stop": 231099, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAATCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAAGTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAACACATTTTTAATTCTTGA", "aa": "MLPSLIKFIALSNPTFKTLNATLGPMPFKLSKSANNFLSSNSLKPYKDQVKSFLISYKHIFNS", "aa_hexdigest": "2f86f56e58c2d4a6a30a4f5d75ffa7b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.271900000002, "isoelectric_point": 10.176221656799317 }, "id": "EFPKPENHBP_469", "locus": "GCA000186005_00434" }, { "type": "cds", "sequence": "contig_1", "start": 231211, "stop": 231393, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526656.1", "SO:0001217", "UniParc:UPI0001A8C12C", "UniRef:UniRef100_C4XEG5", "UniRef:UniRef50_C4XEG5", "UniRef:UniRef90_C4XEG5" ], "nt": "ATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAA", "aa": "MFENYVTTYKSSKEINETLKMLGFKDSLIDDVVRKINLNQDIEFSLEEAIKLMSDKPKTL", "aa_hexdigest": "c6bdaa9e4aa39c8bf37f7321449d566e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12C", "ncbi_nrp_id": "WP_013526656.1", "uniref100_id": "UniRef100_C4XEG5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12C", "RefSeq:WP_013526656.1", "UniRef:UniRef100_C4XEG5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG5", "uniref90_id": "UniRef90_C4XEG5" }, "psc": { "uniref90_id": "UniRef90_C4XEG5", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEG5" }, "pscc": { "uniref50_id": "UniRef50_C4XEG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG5" ], "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein" }, "genes": [ "ruvA" ], "id": "EFPKPENHBP_470", "locus": "GCA000186005_00435" }, { "type": "cds", "sequence": "contig_1", "start": 231412, "stop": 231852, "strand": "+", "frame": 1, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLQPITSNNIVDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRFKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "f2a1161e604ea609c72117339484b6f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 0.993, "score": 279.0, "evalue": 5.88e-95, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "EFPKPENHBP_471", "locus": "GCA000186005_00436" }, { "type": "cds", "sequence": "contig_1", "start": 232103, "stop": 232798, "strand": "+", "frame": 2, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACGAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGA", "aa": "MRKSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISMSTNYVKKYSEINKLTKPNVDIFDNLIKRLKIK", "aa_hexdigest": "9122dc5204b70e2613022a967e5f69c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "query_cov": 0.9653679653679653, "subject_cov": 1.0, "identity": 0.991, "score": 439.0, "evalue": 1.61e-155, "valid": true, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" }, "genes": [], "id": "EFPKPENHBP_472", "locus": "GCA000186005_00437" }, { "type": "cds", "sequence": "contig_1", "start": 233319, "stop": 233450, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGA", "aa": "MNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDE", "aa_hexdigest": "1bc3e1363e697e2abdb23ef609e7e097", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.7792, "isoelectric_point": 6.142328453063963 }, "id": "EFPKPENHBP_473", "locus": "GCA000186005_00438" }, { "type": "cds", "sequence": "contig_1", "start": 233451, "stop": 233786, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_C4XEG8" ], "nt": "TTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAA", "aa": "MFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA", "aa_hexdigest": "05d6c4774ad92cc4040ff46cab4c8f18", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 233289, "stop": 233786, "observations": { "mutation": [ 233448 ], "stop": [ 233448 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEG8", "query_cov": 1.0, "subject_cov": 0.6727272727272727, "identity": 0.991, "score": 216.0, "evalue": 2.87e-70, "gene_start": 1, "gene_end": 111, "reference_start": 55, "reference_end": 165, "reference_sequence": "MNNYKKQGENMNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDEWLFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA" }, "paralog": false, "identity": 0.9878787878787879, "score": 325.0, "evalue": 1.23e-115, "description": "Internal stop codon at 233448. Nonsense mutation around 233448.", "psc": { "uniref90_id": "UniRef90_C4XEG8", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEG8", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12876.668799999981, "isoelectric_point": 8.799241447448733 }, "id": "EFPKPENHBP_474", "locus": "GCA000186005_00439" }, { "type": "cds", "sequence": "contig_1", "start": 233806, "stop": 234165, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKPYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFRKNGDTSCLTVL", "aa_hexdigest": "960afa9397d38dc95b18e550185890cb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14329.662599999978, "isoelectric_point": 9.686004066467284 }, "id": "EFPKPENHBP_475", "locus": "GCA000186005_00440" }, { "type": "cds", "sequence": "contig_1", "start": 234439, "stop": 234657, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "EFPKPENHBP_476", "locus": "GCA000186005_00441" }, { "type": "cds", "sequence": "contig_1", "start": 234692, "stop": 235138, "strand": "+", "frame": 2, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "4164854a7130acf16c96c160bbf5ab07", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 234653, "stop": 235282, "observations": { "mutation": [ 235136 ], "stop": [ 235136 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 9.07e-94, "description": "Internal stop codon at 235136. Nonsense mutation around 235136.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "EFPKPENHBP_477", "locus": "GCA000186005_00442" }, { "type": "cds", "sequence": "contig_1", "start": 235181, "stop": 235282, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 4.829350255693511e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "EFPKPENHBP_478", "locus": "GCA000186005_00443" }, { "type": "cds", "sequence": "contig_1", "start": 235288, "stop": 235428, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSKENNTIPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "cc5458cac4667834d74f24e26816b731", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5374.040500000001, "isoelectric_point": 8.099630546569824 }, "id": "EFPKPENHBP_479", "locus": "GCA000186005_00444" }, { "type": "cds", "sequence": "contig_1", "start": 235776, "stop": 236024, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAAATAACTTTTTTATCACTAAAACCTTCTGCATTTTCCTTTTTTATAGTTGGGTTTTTAGATGGTTTTGGTGGATTAATAGGAAAAGCAAACCAATTACAACTAGTTGCTGTTAAAGCAGTTACAATTGGTAATGTTATTGTTGGTAATATTGTCTTTAATCATATCTTTTTAGTTTTCATATTGCTCCTTATTTGCTATATAAATATTATACTTTATATTTATATTAAAATAAATCTTTAA", "aa": "MAEITFLSLKPSAFSFFIVGFLDGFGGLIGKANQLQLVAVKAVTIGNVIVGNIVFNHIFLVFILLLICYINIILYIYIKINL", "aa_hexdigest": "f50b57062a5a2a7a8f94084e8acefc52", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9064.948599999994, "isoelectric_point": 8.93223972320557 }, "id": "EFPKPENHBP_480", "locus": "GCA000186005_00445" }, { "type": "cds", "sequence": "contig_1", "start": 236272, "stop": 236430, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGA", "aa": "MKYFENKKFEVLDNKEEVEKNHKSPTYIYKNYIECVDALGYFMENYKDPDLK", "aa_hexdigest": "84e4dd2b6bf174ecff57cd3cc33d7ffb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6403.183200000001, "isoelectric_point": 5.264279747009277 }, "id": "EFPKPENHBP_481", "locus": "GCA000186005_00446" }, { "type": "cds", "sequence": "contig_1", "start": 236626, "stop": 236934, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGA", "aa": "MNSKQIQALDKIYLMMEEATRAFYRVKHFENLCKPYNTIGNQINLYVSMINLFKNLYNLKIDTNKTLKEQLQDLKPEVLRALEKSKNALNKLLPIIDSGFGS", "aa_hexdigest": "1cdeb99ea6952a6abf34c664964b4a43", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.784499999983, "isoelectric_point": 9.380423927307131 }, "id": "EFPKPENHBP_482", "locus": "GCA000186005_00447" }, { "type": "cds", "sequence": "contig_1", "start": 237381, "stop": 237758, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5", "UniRef:UniRef50_C4XEH2", "UniRef:UniRef90_C4XEH2" ], "nt": "TTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAA", "aa": "MIKSKKNTSTISDSAFFAFGKEKMKITSLYYANNNPGISVLKNTSTYVLDEFVKYSAIIFGILFAIAFVTSIITIAMNAAFTSKGNNTPAKLKKALNGSLISCGVLLALLILGPGLCGLISNSLK", "aa_hexdigest": "541bd4002d390de57c1f8e6291c84a4a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BE8", "uniref100_id": "UniRef100_A0AB32XAQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAQ5", "uniref90_id": "UniRef90_C4XEH2" }, "psc": { "uniref90_id": "UniRef90_C4XEH2", "uniref50_id": "UniRef50_C4XEH2" }, "pscc": { "uniref50_id": "UniRef50_C4XEH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13307.625799999976, "isoelectric_point": 9.769168281555174 }, "id": "EFPKPENHBP_483", "locus": "GCA000186005_00448" }, { "type": "cds", "sequence": "contig_1", "start": 237899, "stop": 238135, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGA", "aa": "MIFGKPLSNINSYEDLKVLPGAFIGIAIFSICLFFIILAISAFKLSYNKSKESFQSFKASLKAIIPNLVFIIVIPILI", "aa_hexdigest": "c4a50977911cf7020c43ced63351ec3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8654.436699999991, "isoelectric_point": 9.519095420837402 }, "id": "EFPKPENHBP_484", "locus": "GCA000186005_00449" }, { "type": "cds", "sequence": "contig_1", "start": 238289, "stop": 238492, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGA", "aa": "MKDELSLLNSNMGSLFLNSFIAIFALYIVGNITINISINVYQAFFLFIQAPLISVVSIEDGGKKLKV", "aa_hexdigest": "bcb457581ccf759ebf931c004962c8ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7397.688899999997, "isoelectric_point": 5.970674705505371 }, "id": "EFPKPENHBP_485", "locus": "GCA000186005_00450" }, { "type": "cds", "sequence": "contig_1", "start": 238514, "stop": 238975, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGA", "aa": "MLAIMILNLSISFMVLFFSGASKIIKPMSDATGIDSGICALIVYTGALITSLSLNKVMFGIFGVEYTSYLGKFTKKLAAKATKKISKRKSNSKNNSSSEPETTSQTDYHPTGNAKNAELIIIMQNNNQTKKPETTPQTIGAAKKAKKPETLND", "aa_hexdigest": "f013f8d8f6533028ea4dd7abb837224c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16557.087599999977, "isoelectric_point": 9.739512825012206 }, "id": "EFPKPENHBP_486", "locus": "GCA000186005_00451" }, { "type": "cds", "sequence": "contig_1", "start": 239006, "stop": 239143, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAA", "aa": "MDQLSKTLRGEKDLKKTSEISKELSELIENESSIKRIITSMERKK", "aa_hexdigest": "90459cbe5d27ff28c389422274ab21d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5252.027499999999, "isoelectric_point": 9.156267356872558 }, "id": "EFPKPENHBP_487", "locus": "GCA000186005_00452" }, { "type": "cds", "sequence": "contig_1", "start": 239302, "stop": 239748, "strand": "+", "frame": 1, "gene": null, "product": "DUF304 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH4", "UniRef:UniRef90_C4XEH4" ], "nt": "GTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAACCTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTTAA", "aa": "MAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKPSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYS", "aa_hexdigest": "cec5bff51c96bda119173ce659bb621d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 239146, "stop": 239748, "observations": { "mutation": [ 239188, 239278 ], "stop": [ 239188, 239278 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH4", "query_cov": 1.0, "subject_cov": 0.74, "identity": 0.98, "score": 238.0, "evalue": 8.61e-78, "gene_start": 1, "gene_end": 148, "reference_start": 53, "reference_end": 200, "reference_sequence": "MNQNKKISSLKLNIWKNLNIYDAIIILGISGFSAALGFANGLPLWIKIIIFLVAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKTSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYS" }, "paralog": false, "identity": 0.98, "score": 335.0, "evalue": 1.26e-118, "description": "Internal stop codon at 239188, 239278. Nonsense mutation around 239188, 239278.", "psc": { "uniref90_id": "UniRef90_C4XEH4", "product": "DUF304 domain-containing protein", "uniref50_id": "UniRef50_C4XEH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH4", "UniRef:UniRef50_C4XEH4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH4" ], "product": "DUF304 domain-containing protein" } }, "id": "EFPKPENHBP_488", "locus": "GCA000186005_00453" }, { "type": "cds", "sequence": "contig_1", "start": 239818, "stop": 240201, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGA", "aa": "MNKLKQYENEFIKSFETTKIPLNVVTGMELIDFANMIFKKNISKSDKQNYFVEELDTELTKELDMKMQGQKLNDKVIKKLQSDKEYKLISLLAPERVEFNSKYIKVDNEYLSFQTLVDFNSLQINEG", "aa_hexdigest": "54de155b356ae5aa8a0b75791cec2e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14989.076699999989, "isoelectric_point": 5.307647895812988 }, "id": "EFPKPENHBP_489", "locus": "GCA000186005_00454" }, { "type": "cds", "sequence": "contig_1", "start": 240292, "stop": 240627, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGA", "aa": "MSLNEANAETKSNFKMQKISVEQKVLDELVDFINVQKMNLFDSNIIFVNQSKTLEGIKDLETKNDINAKKAFANVYKMHYLQKQALQNSIFIKDDFLNNGIEMSSRNIVYG", "aa_hexdigest": "d468523640bb645f71595be49c8c4145", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12798.509799999985, "isoelectric_point": 6.572315406799317 }, "id": "EFPKPENHBP_490", "locus": "GCA000186005_00455" }, { "type": "cds", "sequence": "contig_1", "start": 240763, "stop": 240894, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGA", "aa": "MPGSGKSATTSKIALHQFVVGNEVIIIDPQREYKDLCKKLDGN", "aa_hexdigest": "fc61c848a8aa12fc080e164cc7532130", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.368800000001, "isoelectric_point": 7.907514762878418 }, "id": "EFPKPENHBP_491", "locus": "GCA000186005_00456" }, { "type": "cds", "sequence": "contig_1", "start": 240901, "stop": 241734, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGA", "aa": "MGKGLETKINPLQINYEIEKDNIKVSDELLISNHTKMVKEFFSLLYPDLDARDLRVANFLLNEFYLSNKIKNIQKLKNTDFPTMKDYIKFLKSYKFKDKKAELYYKDSLNKLSIIFDQDFNNNGIFSNLYNGHTNINFNKTLTVIDTYNLINNIASNEGQASLYLILSYVRNRISNNFFENKTRKICLIVDEAHRFINANNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSDEVMNLTKGMVENIQYTLIFRQNQNGVNAISQMYKSKNELTN", "aa_hexdigest": "280b7bca572fc66e13b2387afc433124", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 49, "aa_cov": 0.16967509025270758, "hmm_cov": 1.0, "evalue": 4.151631598292585e-06, "score": 22.68251609802246, "start": 185, "stop": 231 } ], "seq_stats": { "molecular_weight": 32361.543500000033, "isoelectric_point": 8.832313728332519 }, "id": "EFPKPENHBP_492", "locus": "GCA000186005_00457" }, { "type": "cds", "sequence": "contig_1", "start": 241774, "stop": 241875, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAA", "aa": "MFSIDNKTRFQISIYYNQFEKDMIYKSGDLSNI", "aa_hexdigest": "035e85ec4bfd28e346bfa357462f8ecf", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.5033000000003, "isoelectric_point": 5.9190080642700185 }, "id": "EFPKPENHBP_493", "locus": "GCA000186005_00458" }, { "type": "cds", "sequence": "contig_1", "start": 241917, "stop": 242027, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGA", "aa": "MKKLKNFLLGISSTIPASGVFLISSTNSKNEDSFNN", "aa_hexdigest": "a0e4b77d48cb83e2dc872eacbda44321", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3903.415100000001, "isoelectric_point": 9.524639701843263 }, "id": "EFPKPENHBP_494", "locus": "GCA000186005_00459" }, { "type": "cds", "sequence": "contig_1", "start": 243582, "stop": 243698, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGA", "aa": "MEISNLAPGNEIKLTKNGFYLIYDISLDKTNINKFKIV", "aa_hexdigest": "46b9446de2d42f5c527e5fc58df131d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4359.047700000001, "isoelectric_point": 8.06391506195068 }, "id": "EFPKPENHBP_495", "locus": "GCA000186005_00460" }, { "type": "cds", "sequence": "contig_1", "start": 243734, "stop": 243970, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTGTCAAGATCTTTTTTTGTTGCGTTTAAATCAATAATTTTTTTCTTGGCTAAGTATTTTTCAAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTTTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAA", "aa": "MLISGLISKFSSKDFSIKKLLYSLKISSYFNLSRSFFVAFKSIIFFLAKYFSKSFETLRFLFLQNFLINDGEFASSVK", "aa_hexdigest": "ef675762ed1fa72f7ae50d5f3eee181e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9093.62449999999, "isoelectric_point": 9.997321891784669 }, "id": "EFPKPENHBP_496", "locus": "GCA000186005_00461" }, { "type": "cds", "sequence": "contig_1", "start": 244212, "stop": 244718, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGA", "aa": "MKPIFIKFNNINIEEHIPFNVFVNELINNFNNLIESAKDINNEDIDLNIIELKILKESDENQIIVKAISKDANYYIANNDTYTYNVNLLKSDYSYLLNFNPGTLNVFGLKDKEEIKKYIKELLKNYLIQYNEKIDQSLYKKITIDDINISFNDEELNKIINPKDDDIY", "aa_hexdigest": "25aa009585af386a0ed42f8f656a45a5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19855.2482, "isoelectric_point": 4.60994930267334 }, "id": "EFPKPENHBP_497", "locus": "GCA000186005_00462" }, { "type": "cds", "sequence": "contig_1", "start": 244869, "stop": 245306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGA", "aa": "MENKKDPVKMAQIKNETIRQVREKIIEEFKKRAPKLEIDKLYTIDFTDSFFNNLDLAIGTTLTLKGKHFMLKNPYTVNVINTHSSANKKLNLKDLNFEVITLSEKNLNDFKKALNEKIIDILKKENYPAKEIIKLIEFKLLENQK", "aa_hexdigest": "8d500fe1206dd8cb856ae29ceacad26a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17024.737599999993, "isoelectric_point": 9.435415458679199 }, "id": "EFPKPENHBP_498", "locus": "GCA000186005_00463" }, { "type": "cds", "sequence": "contig_1", "start": 245346, "stop": 245483, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAAAAAAAATTACTTTCATGA", "aa": "MMIQLKEESNLISGDKEFKLINIFTEEMLKEALEIKSKNKKKLLS", "aa_hexdigest": "4b6526b274932fbecbd98c07284bc658", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5284.2575, "isoelectric_point": 8.009826087951662 }, "id": "EFPKPENHBP_499", "locus": "GCA000186005_00464" }, { "type": "cds", "sequence": "contig_1", "start": 245646, "stop": 246392, "strand": "+", "frame": 3, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTAATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKLIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "be7a5766b2b816d133a40d3f81b599cd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 245571, "stop": 246695, "observations": { "mutation": [ 246390, 246534 ], "stop": [ 246390, 246534 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 0.996, "score": 482.0, "evalue": 3.18e-169, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9893048128342246, "score": 739.0, "evalue": 4.37e-273, "description": "Internal stop codon at 246390, 246534. Nonsense mutation around 246390, 246534.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "EFPKPENHBP_500", "locus": "GCA000186005_00465" }, { "type": "cds", "sequence": "contig_1", "start": 246588, "stop": 246866, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTYH", "aa_hexdigest": "5dd97fea0cd43f7cf7d9092425b8453f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8141592920353983, "identity": 0.9890000000000001, "score": 177.0, "evalue": 2.98e-56, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10826.261399999988, "isoelectric_point": 7.949612617492677 }, "id": "EFPKPENHBP_501", "locus": "GCA000186005_00466" }, { "type": "cds", "sequence": "contig_1", "start": 247119, "stop": 247250, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGA", "aa": "MNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFE", "aa_hexdigest": "29bc878b2b3c9cdc4c11e320477ea167", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4674.316799999999, "isoelectric_point": 6.532073402404785 }, "id": "EFPKPENHBP_502", "locus": "GCA000186005_00467" }, { "type": "cds", "sequence": "contig_1", "start": 247353, "stop": 247763, "strand": "+", "frame": 3, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9", "UniRef:UniRef90_C4XEH9" ], "nt": "TTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTTATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATGAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGCCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTAGAACAACAAAAAGTAATAATTATTGGTAAATTAAACTCACCTAAAGTAAAATATAAATTAGTGGAAAATAATAAGGAGTAA", "aa": "MWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKVIIIGKLNSPKVKYKLVENNKE", "aa_hexdigest": "108e7a4df35dd109f76d4b3a54969ad4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 247098, "stop": 247763, "observations": { "mutation": [ 247248 ], "stop": [ 247248 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH9", "query_cov": 1.0, "subject_cov": 0.6153846153846154, "identity": 0.993, "score": 267.0, "evalue": 2.02e-89, "gene_start": 1, "gene_end": 136, "reference_start": 86, "reference_end": 221, "reference_sequence": "MKIHKILMNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFEWYNNDISDVLIKSCAFHYELEFIHPFEDGNGRIGRLWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKVIIIGKLNSPKVKYKLVENNKE" }, "paralog": false, "identity": 0.9909502262443439, "score": 441.0, "evalue": 1.8e-160, "description": "Internal stop codon at 247248. Nonsense mutation around 247248.", "psc": { "uniref90_id": "UniRef90_C4XEH9", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH9", "UniRef:UniRef50_C4XEH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9" ], "product": "Fido domain-containing protein" } }, "id": "EFPKPENHBP_503", "locus": "GCA000186005_00468" }, { "type": "cds", "sequence": "contig_1", "start": 247767, "stop": 247904, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAATTTATAATTGATAAAAGTTTTTTTGAACTTTTTCCTCAAGCTAAACTTGGAGTAGTTTTAATTAATGATATTAAAAGTAATTCAAACAGTCCAAAAGAGCTGCAAGACTATTTAAGAAAAAGTACTTGA", "aa": "MSKFIIDKSFFELFPQAKLGVVLINDIKSNSNSPKELQDYLRKST", "aa_hexdigest": "b1c15633e11f4ecc7a0bc847eb4c2168", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5190.9633, "isoelectric_point": 9.22402362823486 }, "id": "EFPKPENHBP_504", "locus": "GCA000186005_00469" }, { "type": "cds", "sequence": "contig_1", "start": 247992, "stop": 248279, "strand": "+", "frame": 3, "gene": null, "product": "B3/4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C0199FF" ], "nt": "ATGAAAAAAGCAAGATCAAGTGTTGAAATGATGCTTAAAAGAGCAAGTAAAGACGCACAATTTTCTTCAATTAACCCTTTAGTTGATATATACAATAGTGTTGGCTTACGTTTTGCATTTCCTTGTGGCGCAGAGGATATTAATAAATTTAGTGGCAATTTAATTTTAGGCATTACCAAAGGTGGAAATGAATTTTATAGAATTAATAGCACTGAAAATGAACCTGCTTTAGCAGGAGAGTTATGTTATTATGATGATAAAGGTGCTGTTTGTCGTAATTTTAATTGA", "aa": "MKKARSSVEMMLKRASKDAQFSSINPLVDIYNSVGLRFAFPCGAEDINKFSGNLILGITKGGNEFYRINSTENEPALAGELCYYDDKGAVCRNFN", "aa_hexdigest": "75375675ebb57b0e571a599c28ce7cbf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000C0199FF", "query_cov": 0.968421052631579, "subject_cov": 0.8679245283018868, "identity": 0.5329999999999999, "score": 102.0, "evalue": 1.97e-26, "valid": false, "product": "B3/4 domain-containing protein", "uniref50_id": "UniRef50_UPI000C0199FF" }, "pscc": { "uniref50_id": "UniRef50_UPI000C0199FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C0199FF" ], "product": "B3/4 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_505", "locus": "GCA000186005_00470" }, { "type": "cds", "sequence": "contig_1", "start": 248602, "stop": 249189, "strand": "+", "frame": 1, "gene": null, "product": "Abortive phage infection protein", "db_xrefs": [ "RefSeq:WP_013526752.1", "SO:0001217", "UniParc:UPI0001F5A11B", "UniRef:UniRef100_C4XEI1", "UniRef:UniRef50_C4XEI1", "UniRef:UniRef90_C4XEI1" ], "nt": "ATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAA", "aa": "MNYNEKLEKVIEECNGVVTTKECNKRNIPNSIISRAAKKGRIEKYSEGIYVAEEIPMDPHFLFQLRFPRSIFSYESALQLHGVSERFEKALEVTVNTGYKFNDTKKIFPHYVKDEYLELGVEEIKTSQGNKVRVYSLERTFCDMVANRNKVDAETFVKFMITFQGIKINYSQLNSIAKKMGVINKVVDVMGIIRE", "aa_hexdigest": "939390d8773cf80f17abb4e440145976", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F5A11B", "ncbi_nrp_id": "WP_013526752.1", "uniref100_id": "UniRef100_C4XEI1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A11B", "RefSeq:WP_013526752.1", "UniRef:UniRef100_C4XEI1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI1", "uniref90_id": "UniRef90_C4XEI1" }, "psc": { "uniref90_id": "UniRef90_C4XEI1", "product": "Abortive phage infection protein", "uniref50_id": "UniRef50_C4XEI1" }, "pscc": { "uniref50_id": "UniRef50_C4XEI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI1" ], "product": "Abortive phage infection protein" }, "genes": [], "id": "EFPKPENHBP_506", "locus": "GCA000186005_00471" }, { "type": "cds", "sequence": "contig_1", "start": 249215, "stop": 249919, "strand": "+", "frame": 2, "gene": null, "product": "Abortive infection protein AbiGII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2", "UniRef:UniRef90_C4XEI2" ], "nt": "TTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGA", "aa": "MVSKKFDIDYNSIRKLFYLEEILKRIAKSKYRNNFIFKGGMLFSNIIGIENRRTVDIDFSLKGIESNEKNLKIVFNEILLIKNDEINFIFNDIQPIKTDLNYPCYRFKFRASLENEKEDIVLDIGVGDKIFPKPIEYSYKSFFKDESFQVLSYTLETYFSEKLFAIISKGEANTRLKDYYDLHFLILNCKKMIDKSNLGKAISITFDNRNEKFDIKTAKLKLLELSNFELLDNK", "aa_hexdigest": "9028a0862dc82d4cacd999c7fbaffc0f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEI2", "query_cov": 1.0, "subject_cov": 0.8447653429602888, "identity": 0.996, "score": 459.0, "evalue": 1.52e-162, "valid": true, "product": "Abortive infection protein AbiGII", "uniref50_id": "UniRef50_C4XEI2" }, "pscc": { "uniref50_id": "UniRef50_C4XEI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2" ], "product": "Abortive infection protein AbiGII" }, "genes": [], "id": "EFPKPENHBP_507", "locus": "GCA000186005_00472" }, { "type": "cds", "sequence": "contig_1", "start": 250064, "stop": 250453, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGACAACTAAATACGGGCACCAAATTCAAATCGTTGGTGATGACTTAACAGTTACAAACCCTAAATTATTAGCGCGTGCTATTAAAGAAAAAGCAATGAATGCAATTTTAATTAAATTAAACCAAATTGGTTCATTAACAGAAACAATTGATGCTATTAACATGGCTCAAAAAGCTGGAATGGCTTGTGTTGTTTCACACCGTTCAGGTGAAACAGAAGACACAACATTAGCTGATGTTGCTGTTGCATTAAACACAGGACAAATCAAAACTGGATCAATGTCACGTACAGATAGAATTGCAAAATACAATAGATTATTAGCTATTGAAGAAGAATTAGATGGTGCTGATGAATTTGAAGGTCGTGAAGCATTCTACAACATTAAATAA", "aa": "MTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK", "aa_hexdigest": "5b4f7b6538a069aadc8a2cdbe95c8244", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 1.0, "subject_cov": 0.8376623376623377, "identity": 0.628, "score": 164.0, "evalue": 1.48e-49, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "EFPKPENHBP_508", "locus": "GCA000186005_00473" }, { "type": "cds", "sequence": "contig_1", "start": 250469, "stop": 251224, "strand": "-", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_C4XEI3" ], "nt": "ATGACCGCAGTTGATCATGTTAACAAAGATATTGCACCTAAATTATTAGGAATGGAAGTTACAGATCAACGTGCAATCGACATGAAAATGATTAAATTAGATGGCACAGCAACAAAATCAAAATTTGGTGCTAACGCTATCTTAGGAGTTTCATTAGCAGTAGCTAGAGCAGCCGCAGATGAATTACAACTTCCTTTATATAGATACATTGGTGGTACAAATGCCCACGTATTACCATTACCTATGTTAAATGTTATGAATGGTGGAGCTCACGCATCAAACACAGTTGACTTCCAAGAATTCATGATTATGCCAGTTGGTGCTAAAAGCATTCGTGAAGCATTACAAATGGCAAACAAAGTATTCCATAACTTAGCAAAATTATTAAAAGCTGCAGGACACGGAACACAAGTTGGTGATGAAGGTGGATTTGCACCTGACTGCAAAAGTCATGAAGAAGTTCTTGACTACTTAGTTCAAGCTATTAAGGCTGCAGGTTACAAACCAGCTACAAGCGGTAAAGATGCTGTTGCTATTGCAATGGATGCTGCTTCAAGTGAAATTTACGACGAAAAAAGCAAAAAATATGTATTCAAAAAACTTAAAAAAGCTATTGAAGCTAAACACCCAGGTTTTGAAAAACTTACAAATGTTAAACTTGAATACACAACAGATGAAATGATTGAATACTATGGTACTTTAATTGCTAAATACCCAATTATCTCAATCGAAGATGGTTTAGCAGAAGATGACTGA", "aa": "MTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDD", "aa_hexdigest": "7db3fce6604d83ecc25876a608756bf6", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 250166, "stop": 251446, "observations": { "mutation": [ 250471, 251245, 251353 ], "stop": [ 250471, 251245, 251353 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI3", "query_cov": 1.0, "subject_cov": 0.5456521739130434, "identity": 1.0, "score": 487.0, "evalue": 1.52e-170, "gene_start": 1, "gene_end": 251, "reference_start": 75, "reference_end": 325, "reference_sequence": "MKLRRKMSAIYKIHSREILDSRGNPTVQVEVWTEAGGYGLANVPSGASTGSKEALELRDQGTKYEKNWFGGKGVMTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDDWAGFTKMTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK" }, "paralog": false, "identity": 0.9906103286384976, "score": 819.0, "evalue": 6.34e-304, "description": "Internal stop codon at 250471, 251245, 251353. Nonsense mutation around 250471, 251245, 251353.", "psc": { "uniref90_id": "UniRef90_C4XEI3", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI3", "EC:4.2.1.11", "UniRef:UniRef50_P75189", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" } }, "id": "EFPKPENHBP_509", "locus": "GCA000186005_00474" }, { "type": "cds", "sequence": "contig_1", "start": 251723, "stop": 252151, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGA", "aa": "MKKLLTFGMKKENQDIDSAFSRPLQFISNKVIAKLGVGSHLINDYTVTALGHAFCDSIVESEKNNKEFKIFLSNDNTFHALLYTNILARIFDEKGFKSYIFEKNSSYPSVLKNLAAKDDDCDVIISVESFKGLKNIMQISFN", "aa_hexdigest": "2b03d5f235cc455860a869c3ad8112e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16085.282099999982, "isoelectric_point": 7.737003135681152 }, "id": "EFPKPENHBP_510", "locus": "GCA000186005_00475" }, { "type": "cds", "sequence": "contig_1", "start": 252203, "stop": 252496, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGA", "aa": "MNSVNYLSIDIPNQGISFKYNHSPLNYNREILKRYSPLISTFKKQNLKFGVDISQLSTANFYYDTLNRLQIDYFTHNRKKDAYIINANNPDCLKKIF", "aa_hexdigest": "c189ee9498c31e6d549ab6f99fd898df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11473.931199999983, "isoelectric_point": 9.519159889221193 }, "id": "EFPKPENHBP_511", "locus": "GCA000186005_00476" }, { "type": "cds", "sequence": "contig_1", "start": 252893, "stop": 253336, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACCAATGTTGTAATTTTAGAAAGAGAACTTATGGATGGAATTTTAGAATTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGA", "aa": "MLELMRMIAFYKEKSLTLYEVLQQIYDKYGLHHVITKTFNLDDEMASRFIKRILKSETIGNHKIVNFKEYKNATIVNNNIHLKLSFEGGNSITVKYSIIEKEITIDCEAIAKTNSQEDRTNVVILERELMDGILELKEDFKIRKITP", "aa_hexdigest": "1f67790d191061df217ae854bdf05d66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17229.84289999999, "isoelectric_point": 6.914940834045409 }, "id": "EFPKPENHBP_512", "locus": "GCA000186005_00477" }, { "type": "cds", "sequence": "contig_1", "start": 253364, "stop": 253606, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTCAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGA", "aa": "MTFIGVIIFLYFSIYNFKDDSFFGANGDMGEMFKRMGIVIFKDHKTQFAFLSIVLSFFIWTLFNAIIFKRLLDFQGQKVK", "aa_hexdigest": "be23b2457f79a1601612a08498fcf622", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9455.13379999999, "isoelectric_point": 9.455400657653808 }, "id": "EFPKPENHBP_513", "locus": "GCA000186005_00478" }, { "type": "cds", "sequence": "contig_1", "start": 254459, "stop": 254659, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAG", "aa": "MLVFIGEKRVDLYQIKEKNLRLIQNDNMSATTRTKTRFYKISFTIYTIIIIALAITILCPLMGSYK", "aa_hexdigest": "a44c1c53148b18455fec6649fea04e5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7718.2154999999975, "isoelectric_point": 9.841888618469238 }, "id": "EFPKPENHBP_514", "locus": "GCA000186005_00479" }, { "type": "cds", "sequence": "contig_1", "start": 254896, "stop": 255567, "strand": "+", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "nt": "ATGAAAGACTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAATATTACATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGA", "aa": "MKDCTSVGFSAKIQFINKMKGKYYMAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDT", "aa_hexdigest": "3e01d601cd1c824d862be759c3db4bc3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AF59E5", "query_cov": 0.874439461883408, "subject_cov": 0.8297872340425532, "identity": 0.836, "score": 335.0, "evalue": 1.73e-114, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI0021AF59E5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF59E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "product": "GTP-binding protein" }, "genes": [], "id": "EFPKPENHBP_515", "locus": "GCA000186005_00480" }, { "type": "cds", "sequence": "contig_1", "start": 255607, "stop": 256155, "strand": "+", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "GO:0003746", "GO:0005525", "GO:0006414", "SO:0001217", "UniRef:UniRef50_O83217", "UniRef:UniRef90_C4XEI5" ], "nt": "ATGGCTGTTGAAGACGTTTTCACAATCACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACTGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAA", "aa": "MAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK", "aa_hexdigest": "ae0171f1ba10dc3f8e3ac9906b75c5a6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00211B1E30", "query_cov": 1.0, "subject_cov": 0.9479166666666666, "identity": 0.7090000000000001, "score": 270.0, "evalue": 2.93e-90, "valid": false, "product": "EF-Tu/IF-2/RF-3 family GTPase", "uniref50_id": "UniRef50_A0A0A8KZ15" }, "genes": [ "tuf" ], "truncated": "5-prime", "pseudogene": { "start": 255307, "stop": 256155, "observations": { "mutation": [ 255565 ], "stop": [ 255565 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI5", "query_cov": 1.0, "subject_cov": 0.41935483870967744, "identity": 1.0, "score": 365.0, "evalue": 3.78e-124, "gene_start": 1, "gene_end": 182, "reference_start": 253, "reference_end": 434, "reference_sequence": "MFIKVILVNSMDRMQMKDCTSVGFSAKIQFINKMKGKYYMAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDTWIQTPVKEFDKPFLMAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK" }, "paralog": false, "identity": 0.9964539007092199, "score": 556.0, "evalue": 7.45e-202, "description": "Internal stop codon at 255565. Nonsense mutation around 255565.", "psc": { "uniref90_id": "UniRef90_C4XEI5", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_O83217", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI5", "UniRef:UniRef50_O83217", "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_O83217", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O83217" ], "product": "Elongation factor Tu" } }, "id": "EFPKPENHBP_516", "locus": "GCA000186005_00481" }, { "type": "cds", "sequence": "contig_1", "start": 256280, "stop": 256681, "strand": "+", "frame": 2, "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "db_xrefs": [ "GO:0002949", "RefSeq:WP_013526755.1", "SO:0001217", "UniParc:UPI0001F5A25F", "UniRef:UniRef100_C4XEI6", "UniRef:UniRef50_A0A449B9H2", "UniRef:UniRef90_C4XEI6" ], "nt": "ATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGTAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAGCATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCATATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAA", "aa": "MKNLKTFITRDVKVVKKVAQYVLDHLTKSKLVLMNGDLGAGKTTLTKEIAKLLSIDEVITSPTFNYMKVYDGLVHIDAYNLKGDISEFEDYFEDNVVVIEWANRIKHYYKNYLDINITLDKDNNHVFTIKEAK", "aa_hexdigest": "e3374e7e7779f815b6d8b5972d02e756", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F5A25F", "ncbi_nrp_id": "WP_013526755.1", "uniref100_id": "UniRef100_C4XEI6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A25F", "RefSeq:WP_013526755.1", "UniRef:UniRef100_C4XEI6" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI6", "uniref90_id": "UniRef90_C4XEI6" }, "psc": { "uniref90_id": "UniRef90_C4XEI6", "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "uniref50_id": "UniRef50_A0A449B9H2", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B9H2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B9H2" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" }, "genes": [ "tsaE" ], "id": "EFPKPENHBP_517", "locus": "GCA000186005_00482" }, { "type": "cds", "sequence": "contig_1", "start": 256681, "stop": 257241, "strand": "+", "frame": 1, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_013354458.1", "SO:0001217", "UniParc:UPI0001E33008", "UniRef:UniRef100_C4XEI7", "UniRef:UniRef50_N9VF08", "UniRef:UniRef90_C4XEI7" ], "nt": "ATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAA", "aa": "MKLYLDTANEDFVLALLNDKHQVIAQKVLESYPKKVELIPQLTEEILKENNFKIQDFDTFYINIGPGFFTGVRIALVYLRTIVLITKANIKTISTMQILAKQNRRKRSFKINAQGNKYYLYETNKNSAFNYKNIKVETGTLKAYDKVNYFDFLSCFIDYLDEFKSYKDVMDIEPYYIKMPQIGAKK", "aa_hexdigest": "7a8f44009b96a1347d13d10bc328d8dc", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33008", "ncbi_nrp_id": "WP_013354458.1", "uniref100_id": "UniRef100_C4XEI7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33008", "RefSeq:WP_013354458.1", "UniRef:UniRef100_C4XEI7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI7", "uniref90_id": "UniRef90_C4XEI7" }, "psc": { "uniref90_id": "UniRef90_C4XEI7", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_N9VF08", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF08" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "EFPKPENHBP_518", "locus": "GCA000186005_00483" }, { "type": "cds", "sequence": "contig_1", "start": 257241, "stop": 258167, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q4A734", "UniRef:UniRef90_C4XEI8" ], "nt": "ATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTGTTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCTTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCAGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACACTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGTTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGATAATCATCCAGAAATTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAGATTAAATGA", "aa": "MRILGIETSHDDTSIALLEDGKVLALKTISQIDIFKEFGGTIPEISSREHVKNINLIEKIFLQEFDLNTIDYIAYTQKPGLIGTLQIGFLFASALALALNKPLLPINHLEGHFYSAAIKQEIKYPALSLLVSGGHTQLMLVKSPFDIQIIGQTLDDAVGEAYDKVASRLGFGFPGGPIIDKMYQDYKGDFVALTLPHTDGLYDFSFSGLKSQVLNHYNNALQRKKALNKEQLAVSFQETAVTYLINKTKLALDNHPEIKTLVLAGGVSANSELRKRFLKLSTKAIVPNLKYSTDNGAMIAMCAYCKIK", "aa_hexdigest": "76797c6e264aceb77345a7d7f134f2ce", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEI8", "query_cov": 1.0, "subject_cov": 0.9903536977491961, "identity": 1.0, "score": 606.0, "evalue": 4.46e-219, "valid": true, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q4A734", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A734", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A734" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" }, "genes": [ "tsaD" ], "id": "EFPKPENHBP_519", "locus": "GCA000186005_00484" }, { "type": "cds", "sequence": "contig_1", "start": 258442, "stop": 258621, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCATTCAATCTCTCCATTAACCCTTGATGAACATATTGAAAAAATATATGATGCATTTATAAAATTTATAAATGAAAAGTACAACAATGACACCACTACTTTTAGTGCTGAAAATTTCGCAAAAGAATTTAATGAAGTAAATGATGGTAATGGAGTTAAATTAGAGAATGTTTAG", "aa": "MAHSISPLTLDEHIEKIYDAFIKFINEKYNNDTTTFSAENFAKEFNEVNDGNGVKLENV", "aa_hexdigest": "469031b184769c8c4abd8857365a8739", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6767.3659000000025, "isoelectric_point": 4.562602424621583 }, "id": "EFPKPENHBP_520", "locus": "GCA000186005_00485" }, { "type": "cds", "sequence": "contig_1", "start": 258835, "stop": 258981, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAAACTTATCTTTAAAGAACCGCGTTATTTTGAAGATGCAACTCTAAGTGTAGATAATAATAACAAATATAAAATAAGTTTTGGAAATACCAAAATGCATGCTGCTGATTTTGAAAAAATAATTAACATTAATGATATTTGA", "aa": "MPKLIFKEPRYFEDATLSVDNNNKYKISFGNTKMHAADFEKIININDI", "aa_hexdigest": "57638aadb113e50bf9359a2750029b05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5635.3845, "isoelectric_point": 6.533323860168457 }, "id": "EFPKPENHBP_521", "locus": "GCA000186005_00486" }, { "type": "cds", "sequence": "contig_1", "start": 259132, "stop": 259470, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAATGAATTCTTGAATAAATCAATATTTGAATCTACTTTATCAAACGAAAACTTTGAATTTGAAGTTTCAAATCAAAATAATAAACCACAAATTACTTTAAAAATCAAGAAATTTAGACATTTTTCAAAAAAATATTTCAATTGGGACGAAATTGAAAACAATAATTACAACAATGTGTTATATGCAATCACAAAGAAATCAGAAATTATTTTTGATAATGTAGATAATGAATGTGAAACGTTGCTTGACTTAGCAATCAACGATTATATTGAAAAAAGAAAAAATGAAATACTTTTATACTCAGAACGTATGAAAACAACTGATTCATGA", "aa": "MFDNEFLNKSIFESTLSNENFEFEVSNQNNKPQITLKIKKFRHFSKKYFNWDEIENNNYNNVLYAITKKSEIIFDNVDNECETLLDLAINDYIEKRKNEILLYSERMKTTDS", "aa_hexdigest": "8d4c4933ad0dc1aac0b5485f225cf56c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13523.950899999982, "isoelectric_point": 4.929839134216308 }, "id": "EFPKPENHBP_522", "locus": "GCA000186005_00487" }, { "type": "cds", "sequence": "contig_1", "start": 259792, "stop": 260064, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAGAAAAATCATTTGGCTAAAAATATTAGTAATACAGTACCAAATCTATCAAGTGAAACTAATATATCAACTTATGAAAAAAATGATGAAAAAATTATAAGAGTTGAAGATATTAAATTAAATGATACAGGAGCAGAAGATCCAAATACTCATGAAAAAGAATTTGAAAGATTTTTCTTGATTTCTTTAAATGAAAGAAAAATTCGTTCTAATGGTGAAAAAACTGGAATTAAAGAATTAAAATATGATGAAAAAATTACTAAATGA", "aa": "MIKKNHLAKNISNTVPNLSSETNISTYEKNDEKIIRVEDIKLNDTGAEDPNTHEKEFERFFLISLNERKIRSNGEKTGIKELKYDEKITK", "aa_hexdigest": "c4a771f85a435bd438e01046707582df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10494.68619999999, "isoelectric_point": 6.744082832336426 }, "id": "EFPKPENHBP_523", "locus": "GCA000186005_00488" }, { "type": "cds", "sequence": "contig_1", "start": 260920, "stop": 261714, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGGATTTAAACAATGCCAATACTTTAAATATAGTTAAGGAATATTGTGGAATAAAAGATGAAAAAAATATAAATATTGTATTTCAAACTTTTATTAAAAAATACATTTCAAATTCAAAATTAGAATTCATAAGTCGTTTTGAAAATATACTTTCAAATGCGGATTACAACAAAAAAACATTTTATTATGATTCAGAAGTTGCAAAATTATCAGTTGATAAGAAATTCGATAAAATAACAAATTACTTTTACGGTACTAATTCAAATTTTTTTGATTCATTTATAAATGATAAATATACATTTAATATTTCATTAAAAAATATTGGTTTTAAAAAAGATAATACAACATATCTCGACATTAATAATTTAAATAAAGAAATAAGCGTAACATATGATATTGTAAACAAAATTTATGAATTTAATACAATAGGTTTTTTTGCTACAAACAATATCAACAAAATAGTTGATGAATTATTTAAAGAAAAAGATAATATCTTTAAATATCCTTTACAAGATTTAGATTATGAAAAATGTAGAAAAGAAATAATTGAAAAAATAAAAAATGATATTCAAAGTAAATTGACTAATGCTTATATTTCTGAAATTGATAATGAATGTGATTTATCAAAATTGAATATAAAAGCTGGTGTAAATTATCCTTCAGAATACAAAGATAAAAATATAGAAATTGTAACTCTTGAATCAAAAACATTAAAACCATCAGGTGTTTCTGACTTTGATAGTTTTAAAAAATATAACGAATTAAAAAATTATTTAAATAGTTCGATTTGA", "aa": "MPDLNNANTLNIVKEYCGIKDEKNINIVFQTFIKKYISNSKLEFISRFENILSNADYNKKTFYYDSEVAKLSVDKKFDKITNYFYGTNSNFFDSFINDKYTFNISLKNIGFKKDNTTYLDINNLNKEISVTYDIVNKIYEFNTIGFFATNNINKIVDELFKEKDNIFKYPLQDLDYEKCRKEIIEKIKNDIQSKLTNAYISEIDNECDLSKLNIKAGVNYPSEYKDKNIEIVTLESKTLKPSGVSDFDSFKKYNELKNYLNSSI", "aa_hexdigest": "8e6119a6635980c69c115b71b2737a3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30974.611400000045, "isoelectric_point": 5.829770851135254 }, "id": "EFPKPENHBP_524", "locus": "GCA000186005_00489" }, { "type": "cds", "sequence": "contig_1", "start": 261868, "stop": 262089, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTGCAAAATTTTTAAGTATTAACTTTAATGGTCAAACACCATTTACAAAAGAAATATTTAAAAAAAATAATGAAGAATTTAAATTAAATTATTATGACAAACTAGGATACTTAACAAATAAACTTCAAAAAGATGATAATTATTTTAGTGATTTTATTTGTAACCTAAATAGTTTTGGTCTATTCTTTGTAGATTCAAATAAAGCAACTAATTGA", "aa": "MSFAKFLSINFNGQTPFTKEIFKKNNEEFKLNYYDKLGYLTNKLQKDDNYFSDFICNLNSFGLFFVDSNKATN", "aa_hexdigest": "800390bf71359bb1de6d63477cda288a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8632.609499999997, "isoelectric_point": 7.7970159530639656 }, "id": "EFPKPENHBP_525", "locus": "GCA000186005_00490" }, { "type": "cds", "sequence": "contig_1", "start": 262735, "stop": 263259, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAATAAAAAAACAAACATTGATTATCCATTTTATTGTAGTGAACTATTAAAAAATTCTGATTTTAATAATTCGACAGTTTTTGGCAATGAATCAAATAAAGCTGAATTAAAAAATACCAACAATACTTTTAAAATTAGATATGATATTTCTCCAAGAATTAAGATTTTTGATATTTCAATCGATAAAACTAATAAAGAACATGCCGAAGAAGTTGAGTCATTATTAAAGAATAATAAATTAGATGACATTTTCAATAGAAAAATATTTTCAAATAATATTAAAAATGAATTATTACCTCCTGAAAAATTTAGTACTATTAAAGGTGAACCAGGTTGTGATGAGTTTATTGAATCTTACTCAGAAAATAAAAAATATAATACTAAACTAATTTCTTCTAATCCAAATACACCATTGATTAACTTATATTTCATTTCTGAAGACCAAAAAACTTTATACAAAGTACCTGAAAAAGTAGATAAAAATGACCTTGTTTATAATTTGTTTAAAGAATATTGA", "aa": "MENNKKTNIDYPFYCSELLKNSDFNNSTVFGNESNKAELKNTNNTFKIRYDISPRIKIFDISIDKTNKEHAEEVESLLKNNKLDDIFNRKIFSNNIKNELLPPEKFSTIKGEPGCDEFIESYSENKKYNTKLISSNPNTPLINLYFISEDQKTLYKVPEKVDKNDLVYNLFKEY", "aa_hexdigest": "9c710547700f4c05a9c03d0a7c309ff0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20434.69460000001, "isoelectric_point": 5.627708244323729 }, "id": "EFPKPENHBP_526", "locus": "GCA000186005_00491" }, { "type": "cds", "sequence": "contig_1", "start": 263320, "stop": 263448, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAATTGAGCTTGTTAAAGGTCAATGCAATTCAGAATATGATGTGGTTATTTTAAAAATAAACAACATTTATATAAGTTCAAATGATAAATCAATAGTTGAAAAAATAAAAAATGACATTTGA", "aa": "MPRIELVKGQCNSEYDVVILKINNIYISSNDKSIVEKIKNDI", "aa_hexdigest": "46fdcb4130c5689acf9d8b4fbef4e0ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4837.5699, "isoelectric_point": 6.057922554016113 }, "id": "EFPKPENHBP_527", "locus": "GCA000186005_00492" }, { "type": "cds", "sequence": "contig_1", "start": 263854, "stop": 263961, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACAAAAGAAAAAAAGTTATAGCGACAGTGGCAACTGCACTCTCGTTAACAACACTTGGAGTAGTTAGTGCTGCTGCTTTTTACAATGTAAATAGCTGA", "aa": "MIKNKRKKVIATVATALSLTTLGVVSAAAFYNVNS", "aa_hexdigest": "9a5e590a97f90a0d09366438282a78dd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3681.3502, "isoelectric_point": 10.582501411437985 }, "id": "EFPKPENHBP_528", "locus": "GCA000186005_00493" }, { "type": "cds", "sequence": "contig_1", "start": 264313, "stop": 264417, "strand": "+", "frame": 1, "gene": "ydbC", "product": "YdbC family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1", "UniRef:UniRef90_UPI000FF2E50B" ], "nt": "ATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGA", "aa": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVS", "aa_hexdigest": "172154335c0cd7455d67729fa2d6f02c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [ "ydbC" ], "truncated": "3-prime", "pseudogene": { "start": 264313, "stop": 264551, "observations": { "deletion": [ 264505 ], "mutation": [ 264415, 264451 ], "stop": [ 264415, 264451, 264529 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF2E50B", "query_cov": 1.0, "subject_cov": 0.43037974683544306, "identity": 1.0, "score": 70.5, "evalue": 3.25e-15, "gene_start": 1, "gene_end": 34, "reference_start": 1, "reference_end": 34, "reference_sequence": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVSWNEGEAKYDIRDWSEDHLRSSKGITLSLEEFKTIKSFDWQRASKN" }, "paralog": false, "identity": 0.9493670886075949, "score": 139.0, "evalue": 6.89e-45, "description": "Internal stop codon at 264415, 264451, 264529. Frameshift due to deletion around 264505. Nonsense mutation around 264415, 264451.", "psc": { "uniref90_id": "UniRef90_UPI000FF2E50B", "product": "YdbC family protein", "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF2E50B", "UniRef:UniRef50_A0A4P6MMX1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1" ], "product": "Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein" } }, "id": "EFPKPENHBP_529", "locus": "GCA000186005_00494" }, { "type": "cds", "sequence": "contig_1", "start": 264558, "stop": 265355, "strand": "+", "frame": 3, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354463.1", "SO:0001217", "UniParc:UPI0001E33029", "UniRef:UniRef100_C4XEJ2", "UniRef:UniRef50_C4XEJ2", "UniRef:UniRef90_C4XEJ2" ], "nt": "ATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAA", "aa": "MILATKDDKEVILKFLNQEPIYNLLIVGDIMTYGVRTPYLFTYIIKEEEQIKAVILIFNKTVLIYDPEFKVSLKDLKSLIKKHSLINVNLSEKMYQHYQDEFENNGDKYKVHRQTIAILENRYTGDTSLAKKAIFTDSEPIVRSRFLIDEFVDFRGTYQQEVKSYKNALIKKVSTPFIIKDKQNRVASHANILISSDKASMIGGVFTLKEYRQKGYAIQVVGALCNHIFANKRVPMLFFDNPAAAQLYHKIGFKDFGKVFTIKIN", "aa_hexdigest": "811f442de7ab516ae9258b3f107138e7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33029", "ncbi_nrp_id": "WP_013354463.1", "uniref100_id": "UniRef100_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33029", "RefSeq:WP_013354463.1", "UniRef:UniRef100_C4XEJ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ2", "uniref90_id": "UniRef90_C4XEJ2" }, "psc": { "uniref90_id": "UniRef90_C4XEJ2", "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XEJ2" }, "pscc": { "uniref50_id": "UniRef50_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ2" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_530", "locus": "GCA000186005_00495" }, { "type": "cds", "sequence": "contig_1", "start": 265369, "stop": 265986, "strand": "+", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_A0A449BAE8", "UniRef:UniRef90_A0A3P8MDR8" ], "nt": "ATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGA", "aa": "MLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQEL", "aa_hexdigest": "53b37c338906263ae741a9d209ed6956", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ffh" ], "truncated": "3-prime", "pseudogene": { "start": 265351, "stop": 266289, "observations": { "mutation": [ 265984 ], "stop": [ 265984 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MDR8", "query_cov": 1.0, "subject_cov": 0.4466230936819172, "identity": 1.0, "score": 383.0, "evalue": 2.28e-130, "gene_start": 1, "gene_end": 205, "reference_start": 7, "reference_end": 211, "reference_sequence": "MNKGDNMLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQELWDLKKIANPSEIFFVADALSGQDILNVAETFNNKLKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA" }, "paralog": false, "identity": 0.9935897435897436, "score": 587.0, "evalue": 4.33e-213, "description": "Internal stop codon at 265984. Nonsense mutation around 265984.", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR8", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A449BAE8", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MDR8", "EC:3.6.5.4", "UniRef:UniRef50_A0A449BAE8", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAE8" ], "product": "Signal recognition particle protein" } }, "id": "EFPKPENHBP_531", "locus": "GCA000186005_00496" }, { "type": "cds", "sequence": "contig_1", "start": 266086, "stop": 266730, "strand": "+", "frame": 1, "gene": null, "product": "Signal recognition particle protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "nt": "TTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAA", "aa": "MKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA", "aa_hexdigest": "3c1974398956a2d55a7827bd71c8d0d4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PFZ2", "query_cov": 0.9719626168224299, "subject_cov": 0.9285714285714286, "identity": 0.635, "score": 249.0, "evalue": 4.86e-81, "valid": false, "product": "Signal recognition particle protein", "uniref50_id": "UniRef50_A0A3B0PFZ2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PFZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "product": "Signal recognition particle protein" }, "genes": [], "id": "EFPKPENHBP_532", "locus": "GCA000186005_00497" }, { "type": "cds", "sequence": "contig_1", "start": 267057, "stop": 267221, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGA", "aa": "MLLIALSLIFNKLHYVKYYGFLAIISGLLALVKVDFNFTQEEGKDIIFPIGIDN", "aa_hexdigest": "11aa76552efaa29230bbb8baa593fc89", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6133.240400000001, "isoelectric_point": 5.498115348815917 }, "id": "EFPKPENHBP_533", "locus": "GCA000186005_00498" }, { "type": "cds", "sequence": "contig_1", "start": 268414, "stop": 268902, "strand": "+", "frame": 1, "gene": null, "product": "F0F1 ATP synthase subunit A", "db_xrefs": [ "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_C4XEJ6", "UniRef:UniRef90_C4XEJ6" ], "nt": "ATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGA", "aa": "MPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTG", "aa_hexdigest": "5f677a454876725bd33c4273e99f211d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 268333, "stop": 269172, "observations": { "mutation": [ 268402, 268408, 268900, 268906, 268936, 269041, 269146 ], "stop": [ 268402, 268408, 268900, 268906, 268936, 269041, 269146 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEJ6", "query_cov": 1.0, "subject_cov": 0.5806451612903226, "identity": 1.0, "score": 299.0, "evalue": 9.62e-101, "gene_start": 1, "gene_end": 162, "reference_start": 28, "reference_end": 189, "reference_sequence": "MKKTKKFKITRKGGQVEKLTQNLWKWNMPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTGWIWTQIPGLLGEWFFIGSLITPFLHMYFDMFGAIIQALVFCTLTTVYWSKEAVTNENKKQQDAQEKAESEKSVANVENKAVAWSNSSSVY" }, "paralog": false, "identity": 0.9713261648745519, "score": 512.0, "evalue": 3.91e-187, "description": "Internal stop codon at 268402, 268408, 268900, 268906, 268936, 269041, 269146. Nonsense mutation around 268402, 268408, 268900, 268906, 268936, 269041, 269146.", "psc": { "uniref90_id": "UniRef90_C4XEJ6", "product": "F0F1 ATP synthase subunit A", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEJ6", "EC:7.1.2.2", "UniRef:UniRef50_C4XEJ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ6" ], "product": "ATP synthase subunit a" } }, "id": "EFPKPENHBP_534", "locus": "GCA000186005_00499" }, { "type": "cds", "sequence": "contig_1", "start": 269192, "stop": 269428, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354468.1", "SO:0001217", "UniParc:UPI0001E3302E", "UniRef:UniRef100_C4XEJ7", "UniRef:UniRef50_C4XEJ7", "UniRef:UniRef90_C4XEJ7" ], "nt": "ATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAA", "aa": "MTQGGGLVAIGIGIAMLGSFGTGLGQGLAAGKATEAVGRNPEAAAKIRSMLLIGQGVAESSAIYCLVVAFILAFAFTK", "aa_hexdigest": "c899121cf9fca3778600cf0a4ba77d9a", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3302E", "ncbi_nrp_id": "WP_013354468.1", "uniref100_id": "UniRef100_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302E", "RefSeq:WP_013354468.1", "UniRef:UniRef100_C4XEJ7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ7", "uniref90_id": "UniRef90_C4XEJ7" }, "psc": { "uniref90_id": "UniRef90_C4XEJ7", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ7", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ7" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "EFPKPENHBP_535", "locus": "GCA000186005_00500" }, { "type": "cds", "sequence": "contig_1", "start": 269436, "stop": 269960, "strand": "+", "frame": 3, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354469.1", "SO:0001217", "UniParc:UPI0001E3302F", "UniRef:UniRef100_C4XEJ8", "UniRef:UniRef50_C4XEJ8", "UniRef:UniRef90_C4XEJ8" ], "nt": "ATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAA", "aa": "MHTINKSLGTKIEEELNGLFPSVPMLVATLVALAIIILILFFLLYKPIKKSIKARQDYIQSNIDQAKATNDLSKNKLKEANDKLILAHEEANNLVKNAKIRAEKVIISYTAKAKMESKRIIEEAELDIEQQKDSLMEDSKNQIAKAATELSRQILKKEVSKKTESEIIDKFLES", "aa_hexdigest": "36b825b4af6c6c4ced67b7de4879d183", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3302F", "ncbi_nrp_id": "WP_013354469.1", "uniref100_id": "UniRef100_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302F", "RefSeq:WP_013354469.1", "UniRef:UniRef100_C4XEJ8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ8", "uniref90_id": "UniRef90_C4XEJ8" }, "psc": { "uniref90_id": "UniRef90_C4XEJ8", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ8", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ8" ], "product": "ATP synthase subunit b" }, "genes": [ "atpF" ], "id": "EFPKPENHBP_536", "locus": "GCA000186005_00501" }, { "type": "cds", "sequence": "contig_1", "start": 270002, "stop": 270559, "strand": "+", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354470.1", "SO:0001217", "UniParc:UPI0001A8C1A4", "UniRef:UniRef100_C4XEJ9", "UniRef:UniRef50_C4XEJ9", "UniRef:UniRef90_C4XEJ9" ], "nt": "ATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAG", "aa": "MYSKANPEGYALALFELTKEEKDMKAINEVVVSLYNTVVESDKLVMILNDQALEKNKKFDYIDKIFKNISSSKLIVNFLKVLVERNAATLLRRSLEHYLKLSNEFLNIRFALITSAFEISEDKLKKIKQKLEKKYNTEIILKHQIDESLISGFKIKMDSLVIEQNFKKDLQELNTILTKKGGLDG", "aa_hexdigest": "cfc6a7b40b325693d6bb4832d6c95216", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A4", "ncbi_nrp_id": "WP_013354470.1", "uniref100_id": "UniRef100_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A4", "RefSeq:WP_013354470.1", "UniRef:UniRef100_C4XEJ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ9", "uniref90_id": "UniRef90_C4XEJ9" }, "psc": { "uniref90_id": "UniRef90_C4XEJ9", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ9" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "EFPKPENHBP_537", "locus": "GCA000186005_00502" }, { "type": "cds", "sequence": "contig_1", "start": 270552, "stop": 271298, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGA", "aa": "MASRTDDISAIIKDRIRNFDSKVDYSEVGTIITIGDGIALVKGLDNAKNGEIILFKNNIYGLVLNLEEEVVGVTIFGNANSLSEGDTCKRSGEVISIPVGDKLLGRVVNALGQPIDGKGKINTTVKKEIFKVAPGVMTRQEVNQPLETGIIAIDSMIPIGKGQRELIIGDRQTGKTAIAIDTIINQKGKNVNCIYVAIGQKNSTVAQIVKKLEDTGAMEYTTVVVSGASELAPQQYIAPYSGVTIAEE", "aa_hexdigest": "af621f36fcb4a40cce4bae0cd82dd14c", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.967741935483871, "subject_cov": 0.9411764705882353, "identity": 0.604, "score": 302.0, "evalue": 1.09e-100, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "EFPKPENHBP_538", "locus": "GCA000186005_00503" }, { "type": "cds", "sequence": "contig_1", "start": 271299, "stop": 272138, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "ATGGCCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAA", "aa": "MAKGKDCLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSQLLERAARVTKEYGGGSITALPIIETQQGDISAYIPTNVISITDGQIFTKENLFNSGQRPAVDVGFSVSRVGSAAQIKAMKKVAGSLKLELAQYNEMLAFAQFGSDLDDSTKAILQHGAKVYEILKQEQYAPISQIAQVAILLGVKERIINPLPAKYIALYREEVIKYIESDPEGVSIALEIHNNNNEFSDVHFETITKAILKIVKRIIASIPNYDPSDYLPMPEKYSEKSKK", "aa_hexdigest": "638d954f06212560349a21f44ec448d5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.8853046594982079, "subject_cov": 0.8487972508591065, "identity": 0.5870000000000001, "score": 277.0, "evalue": 5.93e-90, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "EFPKPENHBP_539", "locus": "GCA000186005_00504" }, { "type": "cds", "sequence": "contig_1", "start": 272138, "stop": 273010, "strand": "+", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "RefSeq:WP_013354472.1", "SO:0001217", "UniParc:UPI0001E33031", "UniRef:UniRef100_C4XEK1", "UniRef:UniRef50_F9UJQ6", "UniRef:UniRef90_C4XEK1" ], "nt": "ATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAG", "aa": "MAGTQAIKSRISTVESIRKITHAMELVAASKLRRARIEFENVKAYNEVIKETFRSILNHLSPTEIKHLFPIHNIESRMFIIVTSDLGLAGSYNSNIIKFAKNTIKKTDKVILIGRKAISSLASLYENQIVTQFNCDSSEKDYNIVHDIMTIVFELYNRKQVNSINVIYNKYMNNLVQQETCDQVFPFKFDEDKKQKHAEKHKESLNSIIEFEPSAQQVLSESIPLFIDAELYLAYTSAKLSEMAARRSAMESATDNADELIDNLNLEFNRKRQSSITQELNEIVAGADAV", "aa_hexdigest": "bf8dd84f316fdf6e87e135e633de846f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E33031", "ncbi_nrp_id": "WP_013354472.1", "uniref100_id": "UniRef100_C4XEK1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33031", "RefSeq:WP_013354472.1", "UniRef:UniRef100_C4XEK1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK1", "uniref90_id": "UniRef90_C4XEK1" }, "psc": { "uniref90_id": "UniRef90_C4XEK1", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_F9UJQ6", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_F9UJQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UJQ6" ], "product": "ATP synthase gamma chain" }, "genes": [ "atpG" ], "id": "EFPKPENHBP_540", "locus": "GCA000186005_00505" }, { "type": "cds", "sequence": "contig_1", "start": 273020, "stop": 274528, "strand": "+", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354473.1", "SO:0001217", "UniParc:UPI0001A8C1A7", "UniRef:UniRef100_C4XEK2", "UniRef:UniRef50_Q1AVH9", "UniRef:UniRef90_C4XEK2" ], "nt": "ATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAA", "aa": "MAVKKTTTKKVTSAKKSTTKVTITKKLTEKINKNNVGKIIQVLGPVVDVKFNKGFLPSLLNALKVYSNDEVYTLEVAQHIGDDTVRTISMVSTNGLARGLDVIDTGAPISVPVGDKVLGRMFDVLGQPIDLQEAPKAPLMPIHASAPSYEEQKTVSEVLETGIKVIDLLIPYAKGGKIGLFGGAGVGKTVLVQELINNIATEHGGLSVFAGVGERTREGNDLYHEMKAAGVLDKTALVFGQMNEPPGARMRVALTGLTMAEYFRDKENQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERITSTRRGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLDRNIAALGIYPAVDPLESNSRLLDPLVVGQEHYEVAHGVVNILQRFKELQDIIAILGMGELSEEDKLLVARARRIRNFLSQPFNVAEKFSGKKGKYVPISDTIRSFKDILEGKYDDCPEEFFLYVGSIDEVEAKYKEYLAKKEANKN", "aa_hexdigest": "8a9106edcfa70595edde033d65814494", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1A7", "ncbi_nrp_id": "WP_013354473.1", "uniref100_id": "UniRef100_C4XEK2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A7", "RefSeq:WP_013354473.1", "UniRef:UniRef100_C4XEK2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK2", "uniref90_id": "UniRef90_C4XEK2" }, "psc": { "uniref90_id": "UniRef90_C4XEK2", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1AVH9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1AVH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1AVH9" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "EFPKPENHBP_541", "locus": "GCA000186005_00506" }, { "type": "cds", "sequence": "contig_1", "start": 274572, "stop": 274997, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase epsilon chain", "db_xrefs": [ "RefSeq:WP_013354474.1", "SO:0001217", "UniParc:UPI0001A8C1A8", "UniRef:UniRef100_C4XEK3", "UniRef:UniRef50_A0A3P8MDS1", "UniRef:UniRef90_C4XEK3" ], "nt": "ATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAA", "aa": "MLNKKTTHLKITTLEEIFFEGDVRSVTLRTKTGGAICLQPSRTPFFSTIDICELTINSPKDNDFRVCSIGGGLVYADATNINIITDDIIFGKNIDIKRAKIDRDFAMAQLEKFRDTKEEVQYEIKLRKALNRIDVYNRNIK", "aa_hexdigest": "a1ab310e0a3c7964d2ecff8d80619e81", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A8", "ncbi_nrp_id": "WP_013354474.1", "uniref100_id": "UniRef100_C4XEK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A8", "RefSeq:WP_013354474.1", "UniRef:UniRef100_C4XEK3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK3", "uniref90_id": "UniRef90_C4XEK3" }, "psc": { "uniref90_id": "UniRef90_C4XEK3", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A3P8MDS1" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDS1" ], "product": "ATP synthase epsilon chain" }, "genes": [], "id": "EFPKPENHBP_542", "locus": "GCA000186005_00507" }, { "type": "tRNA", "sequence": "contig_1", "start": 275102, "stop": 275175, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "pseudogene": true, "score": 44.9, "nt": "GCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 275134, 275136 ], "id": "EFPKPENHBP_6", "locus": "GCA000186005_00508" }, { "type": "cds", "sequence": "contig_1", "start": 275958, "stop": 276050, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGA", "aa": "MNRNNNLNNYMELVTLGKEYEDRSNRANLA", "aa_hexdigest": "4fce2eaae5b6407c283ff1ba7c0d9ef4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.8830000000007, "isoelectric_point": 6.013872337341308 }, "id": "EFPKPENHBP_543", "locus": "GCA000186005_00509" }, { "type": "cds", "sequence": "contig_1", "start": 276339, "stop": 276626, "strand": "-", "frame": 2, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354476.1", "SO:0001217", "UniParc:UPI0001E33033", "UniRef:UniRef100_C4XEK6", "UniRef:UniRef50_C4XEK6", "UniRef:UniRef90_C4XEK6" ], "nt": "ATGAGTAGAAAAAATAAGAGAAACTATAAAAAAATAATCAATTATGATAAAGCAGAATCAAATGTCATCGACTTACATGGGTTAAATATTTATGAAGCAGTAGCAGAAATTCAATTTGCAATTGCTAATGCTAAAGATAATTCAGAATTTAGCAATAATTACATATTAGCCATTATCGGCAAAGGAACTGAAACTATAAAAGCATATATTGAAGAATTTTTAAGAGATTCAAACTATGAATATATGTTCAATGAGGACTATACCACTATCAAAATATATATTGCTTAG", "aa": "MSRKNKRNYKKIINYDKAESNVIDLHGLNIYEAVAEIQFAIANAKDNSEFSNNYILAIIGKGTETIKAYIEEFLRDSNYEYMFNEDYTTIKIYIA", "aa_hexdigest": "ffc184f34b9af43a1aa7957a0ec31ef5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33033", "ncbi_nrp_id": "WP_013354476.1", "uniref100_id": "UniRef100_C4XEK6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33033", "RefSeq:WP_013354476.1", "UniRef:UniRef100_C4XEK6" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK6", "uniref90_id": "UniRef90_C4XEK6" }, "psc": { "uniref90_id": "UniRef90_C4XEK6", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEK6" }, "pscc": { "uniref50_id": "UniRef50_C4XEK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK6" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_544", "locus": "GCA000186005_00510" }, { "type": "cds", "sequence": "contig_1", "start": 276980, "stop": 277261, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013354477.1", "SO:0001217", "UniParc:UPI0001E33034", "UniRef:UniRef100_UPI0001E33034", "UniRef:UniRef50_C4XEK7", "UniRef:UniRef90_C4XEK7" ], "nt": "TTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MYNEPQIPDFLERGSFLCNSNLKKKKGRKSKINLDAQKTTNFKINIENKIEDKDLLIKQLKEENKILKLENAIAKKMSALVQLKDSLTKKNSK", "aa_hexdigest": "1d2463fd289f62c22a2d77c16b95063d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33034", "ncbi_nrp_id": "WP_013354477.1", "uniref100_id": "UniRef100_UPI0001E33034", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33034", "RefSeq:WP_013354477.1", "UniRef:UniRef100_UPI0001E33034" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33034", "uniref90_id": "UniRef90_C4XEK7" }, "psc": { "uniref90_id": "UniRef90_C4XEK7", "product": "Transposase", "uniref50_id": "UniRef50_C4XEK7" }, "pscc": { "uniref50_id": "UniRef50_C4XEK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK7" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_545", "locus": "GCA000186005_00511" }, { "type": "cds", "sequence": "contig_1", "start": 277327, "stop": 277683, "strand": "+", "frame": 1, "gene": null, "product": "Transposase, IS861", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAATAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFGLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKSLNLFGKTLRRKNKYSSFKGDAHKNIPNLLLEKENIIKDFFRHIRNFSNEKYLDI", "aa_hexdigest": "a43f80955edde4aaaabafb4298700a2b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AEN5", "query_cov": 0.8135593220338984, "subject_cov": 0.8727272727272727, "identity": 0.51, "score": 83.2, "evalue": 1.98e-18, "valid": false, "product": "Transposase, IS861", "uniref50_id": "UniRef50_A0A449AEP7" }, "pscc": { "uniref50_id": "UniRef50_A0A449AEP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_546", "locus": "GCA000186005_00512" }, { "type": "cds", "sequence": "contig_1", "start": 277915, "stop": 278163, "strand": "+", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "nt": "TTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCCAAACATAATTAA", "aa": "MKEKNAIQSMSRKGNCLDNSPTECLFSVIKREFWFGEEKKFNSFKEFKTALEEYISYYNNDRIVNKLKGLSPVQYRNKSKHN", "aa_hexdigest": "2886c266660afe5f4866afcaa139d6a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0003B3129C", "query_cov": 0.9878048780487805, "subject_cov": 1.0, "identity": 0.877, "score": 143.0, "evalue": 3.96e-43, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0003B3129C" }, "pscc": { "uniref50_id": "UniRef50_UPI0003B3129C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "product": "IS3 family transposase" }, "genes": [], "id": "EFPKPENHBP_547", "locus": "GCA000186005_00513" }, { "type": "tRNA", "sequence": "contig_1", "start": 278300, "stop": 278373, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 79.5, "nt": "GCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 278332, 278334 ], "id": "EFPKPENHBP_7", "locus": "GCA000186005_00514" }, { "type": "cds", "sequence": "contig_1", "start": 278473, "stop": 279222, "strand": "-", "frame": 1, "gene": null, "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9", "UniRef:UniRef90_C4XEK9" ], "nt": "ATGGGTGGAATTGAAAATATTACAGGTTTTAATAACTGTGCTGTTCGTCTTAGATATGATGTTAAAGATACTTCATTAGTAAATGAAGAAGCTCTTAAAAAAGTTGGGGCAAGTGAAGTTATTTTTATTGGAAAAAGACATGTGCAAGCTAAATTTGGTGAAATTAGTGAAAAATTAAATTTAGATATTCAAGAAGCTGCACCATTCTTAGAAGAAGAAGCACTTAAAAATAAAAAAATTGAAGTTAAAAGTGATGTCAACTTATTTGAAGAAAAACCGGTTGAAGTTAAAAAAACTGTTGATGAAAATATAGTTTATACACCTTGCAAAGGTACAAGAAAATCAATTGAAGATTTAAGTGATCAAACCTTTAGTTTGCTAGGTTGTGGTTATGCAATTGAAATTGATAAAAAATTAAACAAATTAGAAGTATATGCACCAATTAATGGAAAATTAATTATGTCATATCCAACTAAACATGCATATGGTATAGCAAATGATAATGGTCTTGAAGTTTTAGTACATATTGGTATTGATACAGTTAAATTAAATGGTTTAGGCTTTGAAACAAATCTTAAACAAAATGATAAAGTTGAAAGAGGACAACTCTTAGCAACTGTTGATCTTAAGAAATTAAGAAATAGTAAAATTTGTAGTGATGTTATTGTTTTAATTACAGATAATGTTAATAAAAACAAACAAACAAAATTGCTTTGTCCTTTAGAAATCAAAGAAGTAAATGTTCCTTGA", "aa": "MGGIENITGFNNCAVRLRYDVKDTSLVNEEALKKVGASEVIFIGKRHVQAKFGEISEKLNLDIQEAAPFLEEEALKNKKIEVKSDVNLFEEKPVEVKKTVDENIVYTPCKGTRKSIEDLSDQTFSLLGCGYAIEIDKKLNKLEVYAPINGKLIMSYPTKHAYGIANDNGLEVLVHIGIDTVKLNGLGFETNLKQNDKVERGQLLATVDLKKLRNSKICSDVIVLITDNVNKNKQTKLLCPLEIKEVNVP", "aa_hexdigest": "c2732983f364223a965ba0d7fe98c794", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEK9", "query_cov": 1.0, "subject_cov": 0.8469387755102041, "identity": 1.0, "score": 483.0, "evalue": 1.84e-171, "valid": true, "uniref50_id": "UniRef50_A0A449B3Q9" }, "pscc": { "uniref50_id": "UniRef50_A0A449B3Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9" ], "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component" }, "genes": [], "id": "EFPKPENHBP_548", "locus": "GCA000186005_00515" }, { "type": "cds", "sequence": "contig_1", "start": 279581, "stop": 279793, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00389.36" ], "nt": "ATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGCCGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGA", "aa": "MKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKV", "aa_hexdigest": "24160e7e57cd710d2fea81ee33e9c68b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00389.36", "name": "2-Hacid_dh", "length": 63, "aa_cov": 0.8857142857142857, "hmm_cov": 0.9523809523809523, "evalue": 2.467038179750884e-07, "score": 26.80187225341797, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 8092.2386, "isoelectric_point": 7.8017866134643565 }, "id": "EFPKPENHBP_549", "locus": "GCA000186005_00516" }, { "type": "cds", "sequence": "contig_1", "start": 279806, "stop": 280444, "strand": "+", "frame": 2, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0", "UniRef:UniRef90_C4XEL0" ], "nt": "ATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGA", "aa": "MGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPE", "aa_hexdigest": "861682a6d127cd52b70f2926599b40dd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 279566, "stop": 280618, "observations": { "mutation": [ 279791, 280442 ], "stop": [ 279791, 280442 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL0", "query_cov": 1.0, "subject_cov": 0.6057142857142858, "identity": 1.0, "score": 411.0, "evalue": 6.34e-143, "gene_start": 1, "gene_end": 212, "reference_start": 81, "reference_end": 292, "reference_sequence": "MLGEKMKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKVWLQRSMGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPEWEELISNENVIITSHQAFLTDVALTQIAKITLENADMAQNKDFSKALVIMENGKVKNG" }, "paralog": false, "identity": 0.9914285714285714, "score": 677.0, "evalue": 1.76e-250, "description": "Internal stop codon at 279791, 280442. Nonsense mutation around 279791, 280442.", "psc": { "uniref90_id": "UniRef90_C4XEL0", "product": "D-lactate dehydrogenase", "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL0", "UniRef:UniRef50_C4XEL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0" ], "product": "D-lactate dehydrogenase" } }, "id": "EFPKPENHBP_550", "locus": "GCA000186005_00517" }, { "type": "cds", "sequence": "contig_1", "start": 281019, "stop": 281255, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGA", "aa": "MAKLAMQIVGGILAGLIIYGIGHNASGIKFEANYLANATDTAHKANHYISAHNVSAKSFMPNFDSAFAKEAVITKKTI", "aa_hexdigest": "5de9688470557a7fcbe4adac6461d93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8279.486499999999, "isoelectric_point": 9.399442100524901 }, "id": "EFPKPENHBP_551", "locus": "GCA000186005_00518" }, { "type": "cds", "sequence": "contig_1", "start": 281286, "stop": 281387, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGA", "aa": "MVLGAILLFPIFSPRLDNKYRDTFICFIISFDV", "aa_hexdigest": "2db4f4ce743334ed205d39dad0d1a35c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3865.603400000001, "isoelectric_point": 5.791972923278809 }, "id": "EFPKPENHBP_552", "locus": "GCA000186005_00519" }, { "type": "cds", "sequence": "contig_1", "start": 281388, "stop": 281516, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTATTCTGCTAGGTTCAGCTGCCATTAACCCTGTTCGTGGTTTAGCTCAACAAGTACCTACCTTATTCTTTGATATGAAACACTTAAGTGCTATGGCTCAATATGACATTCACATGGGTACTTGA", "aa": "MGILLGSAAINPVRGLAQQVPTLFFDMKHLSAMAQYDIHMGT", "aa_hexdigest": "463db2fbb911d5eeb66c7da318f8b90c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4545.332000000001, "isoelectric_point": 6.691620445251465 }, "id": "EFPKPENHBP_553", "locus": "GCA000186005_00520" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 282031, "stop": 282203, "strand": "+", "label": "Lysine", "product": "Lysine riboswitch aptamer", "score": 60.4, "evalue": 4.5e-09, "db_xrefs": [ "RFAM:RF00168", "SO:0000035" ], "nt": "AGACGCTATAGAGGAGCTCTTAGTAATAGTATATTAGTTTAGCAGGCATGTGTTGAGACTATATAAAAAGGATATAAGAGCCGAACTGGTGATTGTGGCGGCAATTACTGGTGGTAACAATGCATAACAGTTTGTTACTCTTTGGACCTCAAACCATTGGTGCTATAGCTTTG", "id": "EFPKPENHBP_45" }, { "type": "cds", "sequence": "contig_1", "start": 282280, "stop": 282504, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATCTTCAGTATATTTAGAGTTTACAACACTTCAGTTAGTTTGCCCTTTAATTAAGTCAAGATTAGGTATTACACGGCCTTTTCTTCCACAACTAGCACTGATTGCAACAGGCGCAAAAGCAGCTAATGTAGCTAATGGGGCAAAAGATCATAATAATCTTCTTTTTTTATTCATAATTTCCTTTCGATATATTAATATCTTTTTTAATAATAATTAA", "aa": "MYESSVYLEFTTLQLVCPLIKSRLGITRPFLPQLALIATGAKAANVANGAKDHNNLLFLFIISFRYINIFFNNN", "aa_hexdigest": "5f67508648e3a8f79c47ef80c01fa223", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8351.697699999997, "isoelectric_point": 9.42800159454346 }, "id": "EFPKPENHBP_554", "locus": "GCA000186005_00521" }, { "type": "cds", "sequence": "contig_1", "start": 283085, "stop": 283366, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGA", "aa": "MLSKATKNFTNALRYPNQYLYGLYGVDSNKFKNRSEFLTEKDGREFINFTQLAGKEDITSFQDLLYNVATSKDFVAAPSQYIKETMNSPKISV", "aa_hexdigest": "eae2c119c5f30dca23c210a897b02a19", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10662.89459999999, "isoelectric_point": 8.859325981140138 }, "id": "EFPKPENHBP_555", "locus": "GCA000186005_00522" }, { "type": "cds", "sequence": "contig_1", "start": 283484, "stop": 284017, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTTCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGA", "aa": "MLYAGRYFSAGYYPEASEYRFYKNRFYFDKDFVESEKNFGRVVTKYYSTTEDVFVAQIFDLYQKGKLHTLSFAQLNQQQQLDVTKNPQKYGLEYRQSLNKLNSPFKLLFNTIPYGKNFNADTKNRFLNVHAAKLFYGLTDNQVHNGTTQEETNEDLYVNMLSGRSVAFRSLLTAAVN", "aa_hexdigest": "10f3d075eefbfa456539ad7b720d677d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20701.935900000004, "isoelectric_point": 9.079421043395996 }, "id": "EFPKPENHBP_556", "locus": "GCA000186005_00523" }, { "type": "cds", "sequence": "contig_1", "start": 284399, "stop": 284506, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGA", "aa": "MEKVFKSLESLIKSIDPRLKPSYKKFESSESDKAK", "aa_hexdigest": "c55eec0a479edb08ab97ae7dc1f732df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4074.6954000000023, "isoelectric_point": 9.456109809875489 }, "id": "EFPKPENHBP_557", "locus": "GCA000186005_00524" }, { "type": "cds", "sequence": "contig_1", "start": 284870, "stop": 285076, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTAACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACTTTAATTAATAGTGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGA", "aa": "MSSVISSQFFVSYVKKHTNAQVLTLTKEIMNYMGVVVNPAKTIANENFGPTLINSEYYEVPFAADNNL", "aa_hexdigest": "b4d34c1b5fd13e82d0bcc19f350314ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7590.5722, "isoelectric_point": 5.5737112045288075 }, "id": "EFPKPENHBP_558", "locus": "GCA000186005_00525" }, { "type": "cds", "sequence": "contig_1", "start": 285139, "stop": 285447, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGA", "aa": "MVKYIFKRIAFAILTLFIIIIFSYSIIIAFQQNPYRIAALEEGLSKLEVQQKLQQAEEFDQTPGIVKLFNYLINIFQFNYGEVYVYKSEYETIPEMFFEPLQ", "aa_hexdigest": "3b6c2ef26f2827f064866588a9387984", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12151.050899999995, "isoelectric_point": 4.854925346374511 }, "id": "EFPKPENHBP_559", "locus": "GCA000186005_00526" }, { "type": "cds", "sequence": "contig_1", "start": 285393, "stop": 285626, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAGATATTAAGGAAGAATGGAGCTAAAACAAATGAAGGAACAGCCACGAAGATAAAAATGAAAATGTTTATCAACGTATCTCAAATTGTTCCTCTTTTATATCCAGCTAAAACGCCAATTGCCACACCTAAAATGGCACTAATAACGAAAGCTGGTAATGAAATTAAAATAGTTCATTGTAAGGGTTCAAAAAACATTTCTGGAATTGTTTCATATTCTGATTTATAA", "aa": "MLKILRKNGAKTNEGTATKIKMKMFINVSQIVPLLYPAKTPIATPKMALITKAGNEIKIVHCKGSKNISGIVSYSDL", "aa_hexdigest": "bd6715f103415e5ef31bed2278136e27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8372.052799999998, "isoelectric_point": 10.132060813903806 }, "id": "EFPKPENHBP_560", "locus": "GCA000186005_00527" }, { "type": "cds", "sequence": "contig_1", "start": 285718, "stop": 285939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTCTATTAAGTGGAAGTATTATTATTGAAAGATTCTGA", "aa": "MTLGSLSVYTLYSRNQVITVLTSNYILIAKTKGLSGSKIFFKYVFRNISIPLAAIIIPSYIGLLSGSIIIERF", "aa_hexdigest": "7287c065c528190c491d732c78dba455", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.574099999996, "isoelectric_point": 10.08996295928955 }, "id": "EFPKPENHBP_561", "locus": "GCA000186005_00528" }, { "type": "cds", "sequence": "contig_1", "start": 285940, "stop": 286119, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGA", "aa": "MVKGTSDLIVNAFPKGETNIVMFNILFFTTLSLFTEVLVDVTFTLIDPRIKYSKSGGIN", "aa_hexdigest": "b90260ee76ab9263feebfa26e750f73e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6557.626, "isoelectric_point": 6.000572013854979 }, "id": "EFPKPENHBP_562", "locus": "GCA000186005_00529" }, { "type": "cds", "sequence": "contig_1", "start": 286219, "stop": 286566, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12911.12" ], "nt": "ATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGA", "aa": "MDSRTFNDKYNISDELIQKIKLAEKEKKSTNIVGKPKILFYEICKRFFKNPVVLISFTAFIALLLTGIIVTLTSPYSPTGSILKGGDELTQNLPPVFSPVITNASNYETFQRMKE", "aa_hexdigest": "83bdb90f2be798f2de7b7e896720e0d8", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12911.12", "name": "OppC_N", "length": 59, "aa_cov": 0.4782608695652174, "hmm_cov": 0.847457627118644, "evalue": 1.447959334671112e-08, "score": 30.81315803527832, "start": 41, "stop": 95 } ], "seq_stats": { "molecular_weight": 13009.031499999983, "isoelectric_point": 9.239431571960449 }, "id": "EFPKPENHBP_563", "locus": "GCA000186005_00530" }, { "type": "cds", "sequence": "contig_1", "start": 286665, "stop": 286979, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCAATAATCAAATAACTGAAGGTGGTGCTTGGAAAATTTCAATGACCCTCATCATTACAGTGTCAAGTCATTTTCCAGCATGGAAACCAAGAATAGCACCAACAGCAACACCAATTACAACCTCAATAGTTGCAACAATTAAAGCAACTTTAATTGAGCTAATTGCACCAAATCAAGCGCCTGTTCAAATATCTGCTTCTTTAACTGTTGTACCAAAAAGAGTATTTAAATGTGGCATTGAAGCTTTGATGTTTTCTATATCTTCTGGTTTTAAAGGATTAATTTTTAAAGCACGTGATCAAATATAA", "aa": "MNSNNQITEGGAWKISMTLIITVSSHFPAWKPRIAPTATPITTSIVATIKATLIELIAPNQAPVQISASLTVVPKRVFKCGIEALMFSISSGFKGLIFKARDQI", "aa_hexdigest": "08d882d11204a7f87a87bd4c5ef4d39b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11197.12149999999, "isoelectric_point": 10.132383155822755 }, "id": "EFPKPENHBP_564", "locus": "GCA000186005_00531" }, { "type": "cds", "sequence": "contig_1", "start": 287062, "stop": 287319, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGA", "aa": "MFIITVKDEEYIIAAKSVGASTSRQIFFHALPAILGKIAMNYVRRIPSIILSIASLAFLGFYNKDTDINLGKFIYDNINDAKTNP", "aa_hexdigest": "b1dc4ed1cb0aa22119e4a5794d206c2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9483.959199999985, "isoelectric_point": 9.048863029479982 }, "id": "EFPKPENHBP_565", "locus": "GCA000186005_00532" }, { "type": "cds", "sequence": "contig_1", "start": 287435, "stop": 287758, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "nt": "ATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAATTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATATTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGA", "aa": "MTSVKEKKNIKKRNEDIKAEKLANDLLLKVRNLRVDFKVGRNKYLRIVRGIDLDIFKGQIVGLVGESGSGKSVTSKSLINVNEGATTTADFMQIEDINLNKFKKERQ", "aa_hexdigest": "f9cf7bbf4a1e88555798cfcb2bb8ff90", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FDFF16B", "query_cov": 0.8411214953271028, "subject_cov": 0.8108108108108109, "identity": 0.611, "score": 101.0, "evalue": 7.27e-26, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002FDFF16B" }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFF16B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_566", "locus": "GCA000186005_00533" }, { "type": "cds", "sequence": "contig_1", "start": 287909, "stop": 288271, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9", "UniRef:UniRef90_UPI002FE1EF1F" ], "nt": "TTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCGTCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGA", "aa": "MVGLLDKFGIRDSEQVFDRYPHTLSGGMKQRVVITMVVALKPSLIIADEPTTALDPTVQASVLALFEKIRQEMNISIILISHNISVVAKFCDYIYVMYAGRIVEKGTRKEIFTDPCHPYT", "aa_hexdigest": "abc1a5bcb977d74598da640d6d4e3d40", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0022487FDE", "query_cov": 0.9916666666666667, "subject_cov": 0.8623188405797102, "identity": 0.504, "score": 118.0, "evalue": 7.69e-32, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A838SM00" }, "genes": [], "truncated": "both", "pseudogene": { "start": 287831, "stop": 288556, "observations": { "mutation": [ 287873, 288269, 288458, 288548 ], "stop": [ 287873, 288269, 288458, 288548 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "query_cov": 1.0, "subject_cov": 0.4979253112033195, "identity": 0.8079999999999999, "score": 197.0, "evalue": 9.43e-62, "gene_start": 1, "gene_end": 120, "reference_start": 27, "reference_end": 146, "reference_sequence": "MGKQLLDALNNNEDWKDKTYSEKKNYLLDLLKTFGLRNVEKIFMSYPHTLSGGMKQRIVITMVVALKPDVIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVERGTKQEIFTDPAHPYTWALISAIPESKEEKLYNIKGTPPDMANLPIGDPFAVRSDYALEIDFIKEPPLIPITQTHAAATWLLSPEAPKIEKSDDLKKRLELFKKAFNHEQK" }, "paralog": false, "identity": 0.7219917012448133, "score": 355.0, "evalue": 1.7e-126, "description": "Internal stop codon at 287873, 288269, 288458, 288548. Nonsense mutation around 287873, 288269, 288458, 288548.", "psc": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE1EF1F", "UniRef:UniRef50_A0A223MAZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "EFPKPENHBP_567", "locus": "GCA000186005_00534" }, { "type": "cds", "sequence": "contig_1", "start": 288341, "stop": 288460, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGA", "aa": "MANLPLGDPFAPRNDYALEIDFYKEPPLIEVSETHAAAT", "aa_hexdigest": "67c325ea9b5612a2a08640ce93aaf67e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4317.782400000001, "isoelectric_point": 4.192523765563965 }, "id": "EFPKPENHBP_568", "locus": "GCA000186005_00535" }, { "type": "cds", "sequence": "contig_1", "start": 288543, "stop": 288941, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGA", "aa": "MNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKD", "aa_hexdigest": "2a23d78d0acce62e0e3ffc4d1872fe20", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9242424242424242, "subject_cov": 0.953125, "identity": 0.779, "score": 193.0, "evalue": 1.38e-61, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_569", "locus": "GCA000186005_00536" }, { "type": "cds", "sequence": "contig_1", "start": 289071, "stop": 290894, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6", "UniRef:UniRef90_C4XEL6" ], "nt": "ATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCACAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGA", "aa": "MNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSITDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEE", "aa_hexdigest": "9653af907871df6126bd86e7819f188a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 288771, "stop": 291194, "observations": { "mutation": [ 288939, 289050, 290892 ], "stop": [ 288939, 289050, 290892 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL6", "query_cov": 1.0, "subject_cov": 0.6827896512935883, "identity": 1.0, "score": 994.0, "evalue": 0.0, "gene_start": 1, "gene_end": 607, "reference_start": 183, "reference_end": 789, "reference_sequence": "MERYLKMNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKDWFDIKENFFYVFKQRALELKLKNLESINLLAEKFFPYWIEKFKKMNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSITDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEEWVKPFNINSFSLATNKSIDEEYNSKIDHKPYDGHSEIPNNIEYTDEIKIDEPTCEIKISSNFDFSKPIVEVVETKVSTTKTTKKRSTSRKASSAKKTTKK" }, "paralog": false, "identity": 0.9962825278810409, "score": 1400.0, "evalue": 0.0, "description": "Internal stop codon at 288939, 289050, 290892. Nonsense mutation around 288939, 289050, 290892.", "psc": { "uniref90_id": "UniRef90_C4XEL6", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL6", "UniRef:UniRef50_C4XEL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_570", "locus": "GCA000186005_00537" }, { "type": "cds", "sequence": "contig_1", "start": 291322, "stop": 291693, "strand": "+", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7", "UniRef:UniRef90_C4XEL7" ], "nt": "TTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAA", "aa": "MYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM", "aa_hexdigest": "4f93706ad97d0fb4bb6cc06fbf692075", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 291211, "stop": 291693, "observations": { "mutation": [ 291256 ], "stop": [ 291256 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL7", "query_cov": 1.0, "subject_cov": 0.76875, "identity": 0.992, "score": 214.0, "evalue": 1.62e-69, "gene_start": 1, "gene_end": 123, "reference_start": 38, "reference_end": 160, "reference_sequence": "MTMNSTREYFREAFTWKKLLHLFIILLISLIAGVSLYLYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM" }, "paralog": false, "identity": 0.9875, "score": 298.0, "evalue": 6.93e-105, "description": "Internal stop codon at 291256. Nonsense mutation around 291256.", "psc": { "uniref90_id": "UniRef90_C4XEL7", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL7", "UniRef:UniRef50_C4XEL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7" ], "product": "DUF3899 domain-containing protein" } }, "id": "EFPKPENHBP_571", "locus": "GCA000186005_00538" }, { "type": "cds", "sequence": "contig_1", "start": 291785, "stop": 292870, "strand": "+", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "nt": "ATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGATGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGA", "aa": "MSNLYQTKRDDNILLKAYYDANKNKEIKIDQLNYKTGKITEFLNKKEAYIFLDKNFKNYDQLVDLIDTKIVTKNRNYQIDLESFVNKDFPIESVLKAFYSRIIFYTYNYFNKKKEAPKKNQYTFLLKDNSHKELANKFELIANNRNLVRNLQVMPENFCNSEQLAQYIVNDFKNEKDLKVTVLNKKEIEKLNMGLIISVNKGSTHEPRVVIVEYNGDPNSKEKIVYVGKGITFDTGGMNTKGYHMEGMKYDMSGSVIVAYTVKTLAQLKAKKNVAAIMCITDNRQDGDASLPENVYESMSGITVEVTDTDAEGRLVLADGLYYGAKKLNATTLVDVATLTGAVLRALGTVYSGIYSTDEKN", "aa_hexdigest": "d4d4bd4bd3e170e8dd229e1ff8ff588f", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00068F9161", "query_cov": 1.005540166204986, "subject_cov": 0.9784366576819407, "identity": 0.6940000000000001, "score": 481.0, "evalue": 9.9e-168, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_A0A449ADP4" }, "pscc": { "uniref50_id": "UniRef50_A0A449ADP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "product": "Cytosol aminopeptidase" }, "genes": [], "id": "EFPKPENHBP_572", "locus": "GCA000186005_00539" }, { "type": "cds", "sequence": "contig_1", "start": 292919, "stop": 293149, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAA", "aa": "MPLHEDFHEPNTASIVADLNNYNNDEKSDCNTAAMFLKQFTNDVPYIHCDVAGTADLSGKPQGVLVDTLVEFALLK", "aa_hexdigest": "2d8095404762afb20e369da7dd37587b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.9078947368421053, "hmm_cov": 0.974025974025974, "evalue": 4.5585813538925834e-14, "score": 48.7059440612793, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 8368.247, "isoelectric_point": 4.345022773742676 }, "id": "EFPKPENHBP_573", "locus": "GCA000186005_00540" }, { "type": "cds", "sequence": "contig_1", "start": 293359, "stop": 293850, "strand": "+", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "nt": "ATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACTGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGA", "aa": "MEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQL", "aa_hexdigest": "cd0eed35ea2c69e80d192ebeb0188957", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q6MUD9", "query_cov": 1.0552147239263803, "subject_cov": 0.8349514563106796, "identity": 0.512, "score": 173.0, "evalue": 4.4e-52, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_Q6MUD9", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_Q6MUD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_574", "locus": "GCA000186005_00541" }, { "type": "cds", "sequence": "contig_1", "start": 293875, "stop": 294609, "strand": "+", "frame": 1, "gene": null, "product": "ATPase", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEL9", "UniRef:UniRef90_C4XEL9" ], "nt": "ATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGTCTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGATGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGACTTTTTATTAAATGATAACATTTTTTAA", "aa": "MPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLVYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQRCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFDFLLNDNIF", "aa_hexdigest": "302a1c02505692db7167977c11a47756", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 293575, "stop": 294609, "observations": { "mutation": [ 293848 ], "stop": [ 293848 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL9", "query_cov": 1.0, "subject_cov": 0.5795724465558195, "identity": 1.0, "score": 471.0, "evalue": 3.59e-165, "gene_start": 1, "gene_end": 244, "reference_start": 178, "reference_end": 421, "reference_sequence": "MHEVFMEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQLWKEYCEFGGMPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLVYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQRCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFDFLLNDNIF" }, "paralog": false, "identity": 0.997093023255814, "score": 681.0, "evalue": 2.62e-250, "description": "Internal stop codon at 293848. Nonsense mutation around 293848.", "psc": { "uniref90_id": "UniRef90_C4XEL9", "product": "ATPase", "uniref50_id": "UniRef50_C4XEL9", "kegg_orthology_id": "K07133", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL9", "UniRef:UniRef50_C4XEL9", "KEGG:K07133" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL9" ], "product": "ATPase" } }, "id": "EFPKPENHBP_575", "locus": "GCA000186005_00542" }, { "type": "cds", "sequence": "contig_1", "start": 295048, "stop": 295527, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0", "UniRef:UniRef90_C4XEM0" ], "nt": "ATGGCTAAAGCAGTTTATTTTTCAGATTTAAATTTAATTAAAGTAAAATTCCCAAACAATATTAATAAAGATTTAATTACGAGAGTTGCACATAAATTAGACGATATTTGTGAAGAAATGTGCAATCAAAAAAGCGAAATTAGTTTTTATGTTGAAAAAACCTTTCCATTTGGTCAAAAAATGTTAGCTATTGTTGATGACACACTTGCATATGACTTCAACATTATTTTAAAACCTAAAAAAACTATTAATTTAGAAAGTGCGGATTTCAACAAATATATACTTGAAAAGCTACAAAAACATATTTGTCCATTAGGTTTTAGTACTAAAATTGTTGAAAATTCAAAAAGTTTCAATTTAAAATTTACTGATTTCAAAAATTTTTCAATTGATATTGCTTTATACAGCGAAAATAAAAATAATGAAAGATTTGTTCTAGTTAGATCTAAAGATAAAGCTAATAAAATAGTTTACAATTGA", "aa": "MAKAVYFSDLNLIKVKFPNNINKDLITRVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESADFNKYILEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYN", "aa_hexdigest": "0eb8ef20a1ae7f61cb77e947792d89d7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 294847, "stop": 295563, "observations": { "mutation": [ 294981, 295050, 295554 ], "stop": [ 294981, 295050, 295554 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM0", "query_cov": 1.0, "subject_cov": 0.6680672268907563, "identity": 1.0, "score": 317.0, "evalue": 1.48e-108, "gene_start": 1, "gene_end": 159, "reference_start": 13, "reference_end": 171, "reference_sequence": "MILWKKLEKRKIMAKAVYFSDLNLIKVKFPNNINKDLITRVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESADFNKYILEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYNWTKVYDYEYTGDKVAEIKKYDRIWKETKELFLKKSEKNPDIDNFSHKTAFNLAVDAVYSVFKKYNNF" }, "paralog": false, "identity": 0.9831932773109243, "score": 465.0, "evalue": 4.15e-169, "description": "Internal stop codon at 294981, 295050, 295554. Nonsense mutation around 294981, 295050, 295554.", "psc": { "uniref90_id": "UniRef90_C4XEM0", "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM0", "UniRef:UniRef50_C4XEM0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18536.305899999996, "isoelectric_point": 8.994838523864747 }, "id": "EFPKPENHBP_576", "locus": "GCA000186005_00543" }, { "type": "cds", "sequence": "contig_1", "start": 296238, "stop": 296399, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGGTAATTTATTAACTATTTTAATACCTAAAATTCCCTTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATACTAGAAGCGGAGCTGAAAGCGGAGCTGAAAGCGGAGTTGGAAGTGAGGGGGAAGCAATTTGATTTTTGA", "aa": "MTKGNLLTILIPKIPLKIQEKIVEILERFRILEAELKAELKAELEVRGKQFDF", "aa_hexdigest": "a89219b5f4f496226aef9cac3786dce7", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6207.414399999998, "isoelectric_point": 8.066751670837402 }, "id": "EFPKPENHBP_577", "locus": "GCA000186005_00544" }, { "type": "cds", "sequence": "contig_1", "start": 296409, "stop": 297041, "strand": "+", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "nt": "TTGTTAAATTTTACCAACTTTGATAAAAATAATTCAAAAGAATTACAATCTATAGGTTGTTTTATTAGTGGTTTAAGATCAAAAAATAAAGATAGTTTTGTCAATGGAAACCAAAGATACGTTTCTTATTTGGATGTTTTCAATAACAAAGAAATTAATTATTTACCTAACAATTTTGTAAAAATATTTGATGATGAAAATCAAAACGATCTTAATTATGGCGATGTTATTTTTTGCGGTTCATCAGAAAATTTTGATGAAACAGGTTATGCATCTGTTTACACTATAAAAAATGATGAAAAAGTTTATTTAAATAGTTTTTCATTTATTTTTAGATTTAAAGATAACAATTTATTTTTGCCTAAATTTTCTAAATATTTTTTTAATTGTAAAGACTTTAGAGACTTACTTCTCAAATGTATAAATGGTGTAACTAGATTTAATTTATCAAAAGAAAAGATGTCAAAAATAAAAATACCTATTCCTCCTATTGAAACCCAAAACAAAATAGTTTCAATACTTGATAAACTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAA", "aa": "MLNFTNFDKNNSKELQSIGCFISGLRSKNKDSFVNGNQRYVSYLDVFNNKEINYLPNNFVKIFDDENQNDLNYGDVIFCGSSENFDETGYASVYTIKNDEKVYLNSFSFIFRFKDNNLFLPKFSKYFFNCKDFRDLLLKCINGVTRFNLSKEKMSKIKIPIPPIETQNKIVSILDKLSEYSQEINSGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "c8a0ba698c3d30eab1b49210ab2b2522", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A5B8JXU3", "query_cov": 0.9428571428571428, "subject_cov": 0.8284518828451883, "identity": 0.545, "score": 207.0, "evalue": 2.32e-64, "valid": false, "product": "Restriction endonuclease subunit S", "uniref50_id": "UniRef50_A0A5B8JXU3" }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JXU3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "EFPKPENHBP_578", "locus": "GCA000186005_00545" }, { "type": "cds", "sequence": "contig_1", "start": 297284, "stop": 298258, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2", "UniRef:UniRef90_C4XEM2", "PFAM:PF04200.17", "PFAM:PF13306.12" ], "nt": "ATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAATTTAACCTCACTTGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAAGTTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGA", "aa": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTSLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVS", "aa_hexdigest": "574849dca9cbecf60bc69a9b7c191e4b", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 297284, "stop": 298525, "observations": { "mutation": [ 298256 ], "stop": [ 298256 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM2", "query_cov": 1.0, "subject_cov": 0.542713567839196, "identity": 1.0, "score": 464.0, "evalue": 3.29e-158, "gene_start": 1, "gene_end": 324, "reference_start": 1, "reference_end": 324, "reference_sequence": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTSLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVSWAAFKGCTSLTEINLPENVKFIGKHAFLNCSNLAKVEMKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTTITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTGWDNKDIKSKAFYGCDVLTTVTFASKVKNILKEAFFACPLLKEVSIHKGTDWKTGLMNKNKAFDETITTVKVIN" }, "paralog": false, "identity": 0.9975786924939467, "score": 493.0, "evalue": 4.03e-172, "description": "Internal stop codon at 298256. Nonsense mutation around 298256.", "psc": { "uniref90_id": "UniRef90_C4XEM2", "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM2", "UniRef:UniRef50_C4XEM2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 81, "aa_cov": 0.22530864197530864, "hmm_cov": 0.9259259259259259, "evalue": 2.0805076326438153e-06, "score": 24.336694717407227, "start": 50, "stop": 122 }, { "id": "PF13306.12", "name": "LRR_5", "length": 86, "aa_cov": 0.2654320987654321, "hmm_cov": 0.9883720930232558, "evalue": 4.5281717634222014e-35, "score": 116.65596008300781, "start": 189, "stop": 274 } ], "seq_stats": { "molecular_weight": 34716.519000000044, "isoelectric_point": 8.186598396301271 }, "id": "EFPKPENHBP_579", "locus": "GCA000186005_00546" }, { "type": "cds", "sequence": "contig_1", "start": 298367, "stop": 298858, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "ATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAACAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGA", "aa": "MKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTTITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTG", "aa_hexdigest": "ee906a2dee62d00aa63961c2a8eb06c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 105, "aa_cov": 0.6441717791411042, "hmm_cov": 0.9904761904761905, "evalue": 6.979632271672124e-48, "score": 158.06832885742188, "start": 2, "stop": 106 }, { "id": "PF13855.12", "name": "LRR_8", "length": 46, "aa_cov": 0.27607361963190186, "hmm_cov": 1.0, "evalue": 1.1140623024567465e-15, "score": 53.48106384277344, "start": 49, "stop": 93 } ], "seq_stats": { "molecular_weight": 17336.678299999985, "isoelectric_point": 8.446212577819825 }, "id": "EFPKPENHBP_580", "locus": "GCA000186005_00547" }, { "type": "cds", "sequence": "contig_1", "start": 299215, "stop": 300465, "strand": "+", "frame": 1, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "EC:5.4.2.12", "GO:0006096", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_C4XEM3" ], "nt": "ATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGA", "aa": "MKRTILVVIDGLGLREEKQGNGFKLAKTPTFDKLFKEYPNSILQASGEYVGLPKGQMGNSEVGHLNIGAGTVVYTGLSLINKAMKDGEFEKNKTFNEVFADVKKKNSTLHLMGLLSPGGVHSLEDHLFKLIDMAHAYGLKKVSVHAFGDGRDVAPKSIITSLRKLQTQVDKYGYKIASIGGRFYGMDRDKMFDRVEKHYEALLGNSDHTYESVIDYVNSQYDEDITDEFFIPAIDKGANFVKDNDSIIFFNFRPDRARQLTHLFIGSKLYDNKPKHPVKISKFCSMMKYEGINTLVAFSEMEVKMPIGRVLEKAGKSQLRVAETQKYAHVTYFMDGGNDIEFKNSKRIMVPSLKVESYANAPHMSAEGITDELTKNVLDYDVTIMNYANPDMVGHTGNLKSTIEAVSFLDTQIKRL", "aa_hexdigest": "44271c866e7b4d78b026fe7ca9c32834", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEM3", "query_cov": 1.0, "subject_cov": 0.8205128205128205, "identity": 1.0, "score": 832.0, "evalue": 2.68e-303, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0006096", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "EFPKPENHBP_581", "locus": "GCA000186005_00548" }, { "type": "cds", "sequence": "contig_1", "start": 300580, "stop": 300714, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAA", "aa": "MFICTDKNIKLANGKLANVAPTILDYIGVKQPKEMDEKSLLIRK", "aa_hexdigest": "ea90937aacb199a5afd32d3c427db213", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.905999999999, "isoelectric_point": 9.30203037261963 }, "id": "EFPKPENHBP_582", "locus": "GCA000186005_00549" }, { "type": "cds", "sequence": "contig_1", "start": 300847, "stop": 301278, "strand": "+", "frame": 1, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9", "UniRef:UniRef90_C4XEM4" ], "nt": "ATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGA", "aa": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYD", "aa_hexdigest": "f630fc66a06dddf3e2a4bf7ded7145ae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 300847, "stop": 301581, "observations": { "mutation": [ 301276, 301456 ], "stop": [ 301276, 301456 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM4", "query_cov": 1.0, "subject_cov": 0.4847457627118644, "identity": 1.0, "score": 291.0, "evalue": 1.67e-97, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYDWNKSGEINQDLHKIELFGTSDEIKKAYEELKKLKLKLKVIYFGTHIEITPKGVDKGYGLKWICENIFNCNFDYVMSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY" }, "paralog": false, "identity": 0.9918032786885246, "score": 493.0, "evalue": 1.95e-179, "description": "Internal stop codon at 301276, 301456. Nonsense mutation around 301276, 301456.", "psc": { "uniref90_id": "UniRef90_C4XEM4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM4", "UniRef:UniRef50_A0A3P8L7G9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9" ], "product": "Cof-like hydrolase" } }, "id": "EFPKPENHBP_583", "locus": "GCA000186005_00550" }, { "type": "cds", "sequence": "contig_1", "start": 301498, "stop": 301734, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAG", "aa": "MSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY", "aa_hexdigest": "8c425ea810c83680e259a29abca0aa1d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 49, "aa_cov": 0.6282051282051282, "hmm_cov": 1.0, "evalue": 3.8245136458209925e-17, "score": 59.14257049560547, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8942.941199999994, "isoelectric_point": 5.769976234436036 }, "id": "EFPKPENHBP_584", "locus": "GCA000186005_00551" }, { "type": "cds", "sequence": "contig_1", "start": 302028, "stop": 302189, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGA", "aa": "MALMLVMIILGSYFIAQPDNGSGKNVETYICLIFFGVYGFIITLLLMIYFLIL", "aa_hexdigest": "683c983f6bacf6df334e5565c1924d1f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5998.331799999996, "isoelectric_point": 4.3690088272094725 }, "id": "EFPKPENHBP_585", "locus": "GCA000186005_00552" }, { "type": "cds", "sequence": "contig_1", "start": 302487, "stop": 302585, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVQQRP", "aa_hexdigest": "4cad5b797311cc167a306173bb16a06e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3644.063, "isoelectric_point": 10.834379386901855 }, "id": "EFPKPENHBP_586", "locus": "GCA000186005_00553" }, { "type": "cds", "sequence": "contig_1", "start": 302586, "stop": 302927, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNAQPQNPQPNPYGQPNPQDSGMFQDPNGQDFGGFDPQGN", "aa_hexdigest": "c38993dfc5ccf0d0578af36a1f85a9a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12354.314099999996, "isoelectric_point": 4.5761869430542 }, "id": "EFPKPENHBP_587", "locus": "GCA000186005_00554" }, { "type": "cds", "sequence": "contig_1", "start": 303091, "stop": 304332, "strand": "+", "frame": 1, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q4AAP5", "UniRef:UniRef90_C4XEM6" ], "nt": "ATGGGTTTTAAATTTAAATCAACAGAAATGCGCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGCTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGA", "aa": "MGFKFKSTEMRIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKAQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAK", "aa_hexdigest": "1e1800f8e6ada0a5ec520ecd9eb5f8f0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9782082324455206, "subject_cov": 0.9688249400479616, "identity": 0.557, "score": 439.0, "evalue": 1.75e-149, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 303088, "stop": 304635, "observations": { "mutation": [ 304330 ], "stop": [ 304330 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM6", "query_cov": 1.0, "subject_cov": 0.49108204518430437, "identity": 0.998, "score": 800.0, "evalue": 1.64e-285, "gene_start": 1, "gene_end": 413, "reference_start": 2, "reference_end": 414, "reference_sequence": "MMGFKFKSTEMLIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKAQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAKWKAVTEKIKELYEIGQPVLVGTAQIEDSEILHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKKWREQAIRNSNDDTDYINWYEKDLVLEIVDIYWQNHIDTMDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN" }, "paralog": false, "identity": 0.9941747572815534, "score": 1000.0, "evalue": 0.0, "description": "Internal stop codon at 304330. Nonsense mutation around 304330.", "psc": { "uniref90_id": "UniRef90_C4XEM6", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q4AAP5", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM6", "EC:7.4.2.8", "UniRef:UniRef50_Q4AAP5", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q4AAP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4AAP5" ], "product": "Protein translocase subunit SecA" } }, "id": "EFPKPENHBP_588", "locus": "GCA000186005_00555" }, { "type": "cds", "sequence": "contig_1", "start": 304420, "stop": 305184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "TTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGA", "aa": "MHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKK", "aa_hexdigest": "9aa2252219956fa6b5019b8302c8044b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 54, "aa_cov": 0.2125984251968504, "hmm_cov": 1.0, "evalue": 5.17856445568776e-48, "score": 159.6897430419922, "start": 54, "stop": 107 } ], "seq_stats": { "molecular_weight": 29412.01260000001, "isoelectric_point": 7.811456871032715 }, "id": "EFPKPENHBP_589", "locus": "GCA000186005_00556" }, { "type": "cds", "sequence": "contig_1", "start": 305296, "stop": 305613, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAA", "aa": "MDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN", "aa_hexdigest": "966fb897797673cecf218e001389645b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12499.864199999993, "isoelectric_point": 4.600741386413574 }, "id": "EFPKPENHBP_590", "locus": "GCA000186005_00557" }, { "type": "cds", "sequence": "contig_1", "start": 305780, "stop": 305905, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGA", "aa": "MNTPQDNKNHEDKRVQVEIKRSKVKQSFLHFGSLYRVKKLR", "aa_hexdigest": "3f65eaa5943d982a099af793f7aab63e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4983.7145, "isoelectric_point": 10.446279716491699 }, "id": "EFPKPENHBP_591", "locus": "GCA000186005_00558" }, { "type": "cds", "sequence": "contig_1", "start": 306584, "stop": 306982, "strand": "+", "frame": 2, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE", "UniRef:UniRef90_UPI000FF5F3CE" ], "nt": "ATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAG", "aa": "MVLINGVVLNVLFPKYKIVKVEIYSSKIPEIQEKINTLKDVRFATSHLSVKGGYSGQEQEVLVTMCLYLDAAHLLEIVREFDENALFTVTDLKKVDGYFYVSVENHKSLFNKKIENKEQKKTKENVEDNSKK", "aa_hexdigest": "d0b89e52f80b83486cc237f32fe05585", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF5F3CE", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.992, "score": 246.0, "evalue": 2.92e-82, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI000FF5F3CE" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5F3CE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_592", "locus": "GCA000186005_00559" }, { "type": "cds", "sequence": "contig_1", "start": 307044, "stop": 307307, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGA", "aa": "MENKNNQNELTNQNEIEIQRSKIKQSFLHFGTLYRVRSFKKQIIYTIIIGVLFGIVQHLLISITGLYNMGLDGLCQSIARIVRHFLR", "aa_hexdigest": "a108be0960c4f784d15c099e102f04d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10177.83499999999, "isoelectric_point": 10.006669807434083 }, "id": "EFPKPENHBP_593", "locus": "GCA000186005_00560" }, { "type": "cds", "sequence": "contig_1", "start": 307362, "stop": 307532, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATAATGTGTTATTCTGGCTGTTAATATTAGTAGCTAATATTCCTTTATTTGTTTTAGCATATAAAAAAATTAGCAAAAAATTTGGTTTCTTATCTATTATTTTCTTAGTTGTAGCTTGTATTACAGGTTTTTGTATTGGTTTAATTCCAACCTCTGAGCAATGA", "aa": "MIYNVLFWLLILVANIPLFVLAYKKISKKFGFLSIIFLVVACITGFCIGLIPTSEQ", "aa_hexdigest": "c7ff9c1a082520c4c88ea1257a88b440", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6323.783299999997, "isoelectric_point": 9.185020256042481 }, "id": "EFPKPENHBP_594", "locus": "GCA000186005_00561" }, { "type": "cds", "sequence": "contig_1", "start": 307755, "stop": 308279, "strand": "+", "frame": 3, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD", "UniRef:UniRef90_UPI00217FD9AD" ], "nt": "TTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAA", "aa": "MLFLFACNLIGSYIPFAVEDPIAASFSKHPGNRYELENFFNPSFVAGFIMVVVNGIVLNVLFPKFKMVKVEIISNQVEMIQQKLNTLKDRRYATSTLTVIGGYSHKEQKILLTTCLYIDAAKLLETVRGIDQNALFTVMDLKKADGYIYTTHEHQNHLFKHKKRDEINDQQKQN", "aa_hexdigest": "a5363b2b2ebc89e51b58f1a7dc4717ad", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00217FD9AD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 337.0, "evalue": 7.01e-117, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI00217FD9AD" }, "pscc": { "uniref50_id": "UniRef50_UPI00217FD9AD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_595", "locus": "GCA000186005_00562" }, { "type": "cds", "sequence": "contig_1", "start": 308700, "stop": 309026, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACAAAAACATGAGAATTGATATTAATCCAAAAAATTATACTTTTACTTTAAAAAAATTAGGTAATAATAAAAAGGAAAAACCAATTAATCTAACTTCGCTTAAATTAACAAAAGAAGCAATTGAAGCTTACAAAAAAACTGGATTGAAAAAACCATCTCAAGTTATAACACATCCTTCAAAACAAATTACAGGTAAAAATGCAGGTTCTCATGGATTTTTATATACAGTTCTTGATAAAAATGGCAAAGTCAACATTATATTTTCAATTAAAGAAAATAATGTAAAATCTAAAGACTTCTTAGCTAAAATTGAAGACTAG", "aa": "MNNKNMRIDINPKNYTFTLKKLGNNKKEKPINLTSLKLTKEAIEAYKKTGLKKPSQVITHPSKQITGKNAGSHGFLYTVLDKNGKVNIIFSIKENNVKSKDFLAKIED", "aa_hexdigest": "81037d5d29170d4cc97526bc406084bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12219.110099999985, "isoelectric_point": 9.999384880065918 }, "id": "EFPKPENHBP_596", "locus": "GCA000186005_00563" }, { "type": "cds", "sequence": "contig_1", "start": 309261, "stop": 310037, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAACAAATGTTGACTTAAATGAAAAAGCAAGTAAAACAATTGAAGAATATATTGCAGCTCACACAAGTGATTTATTGGCTCAAGTTACAGCCACAACTCCAAGTGGTAAAACCTTAAGCGAATATCTAAGTGAAGAAAAGATTACAATTAATAAAACAGAATTAATTGTTGCTAATAATAATTTAGGTCAAGCAAAATTGAAATTAACTTTAAGTTTTGAAGATAAAAGTCAAGAAAATGTTGAAGTTGAATATGAAATTAATGATTTCAAAAAACCAGCTCCATATTCAGAAGACAAATTTGAAAACTTAATCGGTACTTTAAAAACTAAAATTAGTGAAAAGAACTATTATTCAGAAATTAAAGAAGAACAAAATTTAGTAAAACTTTACTACACTGATCCAGATACAAATGAAGAAAAAGAATTCAATAAAGAAAATCTTGAAAAATTGAATATTCAAGCCACAACAATTAATTTAAATAATTTAATGACATTAAATTTTGATGATGCAACACGTAAATTAACTATTACGGTTTCATACACAACTAAATCAACTCCAAAAGAAAAGAAATTTGAATTAACTTTTGCAGAAAAATCATTTGTAGAATTAAAAGATACATTTTTAGCTGAAATTAATCTTTCATTACCTAACCCTTGTGATGTTACAAAGGCTACAGATAATGACATCATCTATGAAAAAACTAAATTAAATGTACCTACAACAACAAATGACATTTTCACATTAACTGGCGTAACTGTTGATTGA", "aa": "MAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKISEKNYYSEIKEEQNLVKLYYTDPDTNEEKEFNKENLEKLNIQATTINLNNLMTLNFDDATRKLTITVSYTTKSTPKEKKFELTFAEKSFVELKDTFLAEINLSLPNPCDVTKATDNDIIYEKTKLNVPTTTNDIFTLTGVTVD", "aa_hexdigest": "658942cfc30296747c60896b1b1d6c01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29300.57820000006, "isoelectric_point": 4.775009727478028 }, "id": "EFPKPENHBP_597", "locus": "GCA000186005_00564" }, { "type": "cds", "sequence": "contig_1", "start": 310116, "stop": 310334, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAAAAGATAAATCAACTAAAACTGCACAAGAATATGTTGAAGAATTTAATGCACTAACTGATCAAGAAAAAGAAGAAAAAATTACATTCACAAATGCAGATTATCAAAATGATTTATCTGTATTTAAAAACATGGAAGGTTTAAATTACATTAAATATCAATTAGAAGTTGTTGACAATTTTGAAGGAAAAGTTAAATTAGTTGCAAAATGA", "aa": "MDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLSVFKNMEGLNYIKYQLEVVDNFEGKVKLVAK", "aa_hexdigest": "1ff10af9a0e02a06a1b071213972da6d", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8431.336199999998, "isoelectric_point": 4.61694049835205 }, "id": "EFPKPENHBP_598", "locus": "GCA000186005_00565" }, { "type": "cds", "sequence": "contig_1", "start": 310458, "stop": 311375, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1", "UniRef:UniRef90_C4XEN1" ], "nt": "ATGAAGAAGAAAATTAAATTAAGTCTTATTTTTTCAAGCGTAACAGCAGGTGTTTTACCTTTAATTTCATCTCAATGTCAAAACACAACAAAAGAAGAAATTAAAGCAGATGAAGACTTTAAACTTTTGTCTATTGCAGATATTAAGAATGCTGATTATGACTCTACAAACAAAGTATATAAGATTAGCACAACTACAAAAGAAGCTGATCAAATTAAAAAAGAAGATATTAAATTTGAAAGTACAACAAAGAAATATACATATATAATTGACAACTTAAAAGCAGATAAAGCTAAGGGTGAATTAGAAATTTTCCTTACTCAAAAAGAAGGAAATAAAGAAATTAAAAAATTCACAATTAAGGTTGTAGGATTTTATGTAGATACTGACAAATTTGACGAAAACATCAAATTTATAACTGGTTTGGACTTAGCAGAAAAAGACAAATACACAGTTAAAGAATACAAAAATCAATTTAATGATCTAGCTAAATTACAAGCTAAATTGTCAATAGCAACTTCAAATAGCAAAGATTTAGCTACTTTCTTAAAAGAATATGAAATATCAATTAAAAGCATAGAAATAGAAGAAGTAAGTGCTTCAAAAGGTGAAGCAAATATTAAAGCAACTTTTGAAAATAAAGCAAAAACTAAATCAAAAGAAATTACAATCAAATTAACTGGATTCAAAAAAGAAACTGAATCGTTACAAGCAACATTAGAAGGTATTTTTGATACTATTGATGTTTTAGAAAACAAAAAAGATTTCAATGTTTTCAACTATACAATTACAGATCTAAGATACTTCAAAAATAAAACACAAGTTGATGTAACAAAAGTATTTAATGAAAAAGGTATTGAAATTAAAAATAAAGAAGCTGATACCTCATCAGGTGAAACTGAACCTTATTCAGGATGA", "aa": "MKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSG", "aa_hexdigest": "ae850df99fd388f214e58ee19d300b84", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 310155, "stop": 311396, "observations": { "mutation": [ 310400, 310433, 310460 ], "stop": [ 310400, 310433, 310460 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN1", "query_cov": 1.0, "subject_cov": 0.5360281195079086, "identity": 1.0, "score": 558.0, "evalue": 3.09e-196, "gene_start": 1, "gene_end": 305, "reference_start": 8, "reference_end": 312, "reference_sequence": "MLMKEKYMKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSGWLSINITFGWKDATKTETYVWSQVVAGFKEIKVNSELEAAFNVDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLSVFKNMEGLNYIKYQLEVVDNFEGKVKLVAKWQQWNQPEYAYNKEITGFVTKETIQQKMAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKIS" }, "paralog": false, "identity": 0.9903147699757869, "score": 786.0, "evalue": 1.9e-288, "description": "Internal stop codon at 310400, 310433, 310460. Nonsense mutation around 310400, 310433, 310460.", "psc": { "uniref90_id": "UniRef90_C4XEN1", "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN1", "UniRef:UniRef50_C4XEN1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1" ], "product": "Lipoprotein-associated type-17 domain-containing protein" } }, "id": "EFPKPENHBP_599", "locus": "GCA000186005_00566" }, { "type": "cds", "sequence": "contig_1", "start": 311660, "stop": 311878, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGA", "aa": "MTRKTKIGILIGTNIIVTAGLITALAIAPTRNNSNHSNSGTTTSETRKIEVKAKVSVDLSKKIDQLSNPSTE", "aa_hexdigest": "f7a31823f30009d5ceca04e301345f8a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7649.6922, "isoelectric_point": 10.11536350250244 }, "id": "EFPKPENHBP_600", "locus": "GCA000186005_00567" }, { "type": "cds", "sequence": "contig_1", "start": 313312, "stop": 315321, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_013354505.1", "SO:0001217", "UniParc:UPI0001A8C1E6", "UniRef:UniRef100_C4XEN3", "UniRef:UniRef50_Q8EUN2", "UniRef:UniRef90_C4XEN3" ], "nt": "ATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAA", "aa": "MSIFKLISPYKPAGDQPEAIKELVENIKDNMQHQVLKGVTGSGKTFTIANVIKEFDRPVLVLSHNKTLASQLYSELKGFFPHNRVEYYVSYFDYYRPEAYIPSSDTYIDKQSQNNKDLDAMRMSAMNAVLTRNDTIIVASVSAIYGALNPSEYYASVFPIEVGMQIKRQEFFKKLVQRNYKRNQIDLELGTFSAKGDAVYIQPADSDEYQIRVDFFGDEIDSIATCDPITKEVIERFSHYTIFPGDAYTVDRSVIDEACDKILVELDERIKYFKANNQLIEAQRIQERVEKDVDSLREFGMCPGIENYARYMDRREAGERPYTLLDYFKGKKPLLFIDESHMMIPQLNGMYHGDYSRKKNLVDYGFRLPSALDNRPLLFKEFENEFDFQTVYISATPDDYELDKTHGLLTKLFVRPTGLLDPKIEIRLSKNQIEDIYDELQRQKDKNERTLILTTTKRLAEELSRYFLEKNEKIAYIHSEHNTFERNEILRKLRKGVYDTVIGINLLREGVDLPEVSLIMVLDADKEGFMRSTKSLIQISGRAARNANGRVIFYADKTTKSMQECIEDNEIKRKMQIAYNKKHNITPKTIIKPIAEPIHGEGIEGEIEFFFKNEKRNQTQSGKKAKEELIKRLREQMNQAAKELDYERAIELRDIILELQAGKDKKVEE", "aa_hexdigest": "dbfa3505caad1d745101853c8b8ae6bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1E6", "ncbi_nrp_id": "WP_013354505.1", "uniref100_id": "UniRef100_C4XEN3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1E6", "RefSeq:WP_013354505.1", "UniRef:UniRef100_C4XEN3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEN3", "uniref90_id": "UniRef90_C4XEN3" }, "psc": { "uniref90_id": "UniRef90_C4XEN3", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_Q8EUN2", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUN2" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "EFPKPENHBP_601", "locus": "GCA000186005_00568" }, { "type": "cds", "sequence": "contig_1", "start": 315324, "stop": 315566, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4", "UniRef:UniRef90_C4XEN4" ], "nt": "ATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGA", "aa": "MRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVN", "aa_hexdigest": "da113e026a68a00f28314dbbeadff8ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 315318, "stop": 315830, "observations": { "mutation": [ 315564 ], "stop": [ 315564 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN4", "query_cov": 1.0, "subject_cov": 0.47058823529411764, "identity": 1.0, "score": 156.0, "evalue": 3.67e-47, "gene_start": 1, "gene_end": 80, "reference_start": 3, "reference_end": 82, "reference_sequence": "MKMRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVNWAVNFIKDLKLELNKVNDIEGFLDLVLTQKNQKLIKQKLSQIFKFVNLDGNVKDIIIIYDLICFIGLLVNLTNTEFELLPYDACNIIS" }, "paralog": false, "identity": 0.9882352941176471, "score": 329.0, "evalue": 9.77e-118, "description": "Internal stop codon at 315564. Nonsense mutation around 315564.", "psc": { "uniref90_id": "UniRef90_C4XEN4", "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN4", "UniRef:UniRef50_C4XEN4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9398.959099999995, "isoelectric_point": 9.380746269226073 }, "id": "EFPKPENHBP_602", "locus": "GCA000186005_00569" }, { "type": "cds", "sequence": "contig_1", "start": 315846, "stop": 316340, "strand": "+", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "nt": "ATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGA", "aa": "MSAKEEIIVKGAREHNLKNVSVTLPKNKLIVFTGLSGSGKSSLAFNTIYEEGRRKYVDSLSNYARLFLGGTSKPDVDSIDGLSPSISIEQKTTHNNPRSTVGTVTEIYDYFRLLFARIGKPFCPHHNIEITSQTNKDIIDSVFNLKDGSKLYILAPVVDGEKGT", "aa_hexdigest": "9d7e153b83907ee5c6a8c1cceb991b4e", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI0021D31D26", "query_cov": 0.9817073170731707, "subject_cov": 0.8944444444444445, "identity": 0.6459999999999999, "score": 218.0, "evalue": 6.86e-70, "valid": false, "uniref50_id": "UniRef50_A0A848YIN1" }, "pscc": { "uniref50_id": "UniRef50_A0A848YIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "product": "UvrABC system protein A" }, "genes": [], "id": "EFPKPENHBP_603", "locus": "GCA000186005_00570" }, { "type": "cds", "sequence": "contig_1", "start": 316473, "stop": 316742, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGA", "aa": "MVLNEENRNRISEALSVAVEYGKGIVKVETTDDEVKTFSKLHSCVYKDFEMPKIETKLFSFNSPYGMCQNCKGLGVDLKADFDALTPEP", "aa_hexdigest": "fc551f295422ab42edea8ee30980c0e5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10011.31079999999, "isoelectric_point": 4.910002326965332 }, "id": "EFPKPENHBP_604", "locus": "GCA000186005_00571" }, { "type": "cds", "sequence": "contig_1", "start": 317052, "stop": 318668, "strand": "+", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "ATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATTAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAA", "aa": "MGTFECSTCHGSRLNEYALAVRINGYNIEDFSRMSVEDALITIENLKLNEEETKISTLIINELINRLSFLKNVGLNYLTLNRNAETLSGGESQRIKLATQIGSNLTGVLYVLDEPSIGLHQKDNDRLIKTLKSMVEIGNTLIVVEHDEETIRSADYIVDIGPKAGIHGGEIIAQGTLEDIIKEPKSITGKYLSGEMKIEVPRVRRSGNGKVVTIFQAAENNLKNIDVKFPLGKFIGVTGLSGSGKSSLVNEILVKGIESVLGHKTGKIGKFKSISGLNQIDKIVPISQSPIGRTPRSNPATYTSVFDDIREIFASVEESRARAYSRSRFSFNVPGGRCEKCQGDGSIKIEMHFLPDVYVPCDECDGQRYNRETLEIKYKNKNIAQVLDMTADEALEFFSTRANIRIKLETLVAVGLGYIKLGQSSTTLSGGEAQRVKLATYLQKKATGKTIYVLDEPTTGLHSYDVANLLKVLNKIVDNGDTVVVIEHNLDVIKCCDYIIDLGPEGGVNGGRVVATGTPEQVAENENSYTGQYLKMIL", "aa_hexdigest": "c9f1e1ab50166988e5f6cd6d899b9299", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XVF5", "query_cov": 1.0111524163568772, "subject_cov": 0.9963369963369964, "identity": 0.691, "score": 757.0, "evalue": 4.79e-271, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "EFPKPENHBP_605", "locus": "GCA000186005_00572" }, { "type": "cds", "sequence": "contig_1", "start": 318975, "stop": 319079, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGA", "aa": "MDVATKKYVNKYPYKVRKYGPITERRDYSVTKIT", "aa_hexdigest": "5c7091208219028974a42cfdee7e2a1e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4112.751300000001, "isoelectric_point": 9.917123222351073 }, "id": "EFPKPENHBP_606", "locus": "GCA000186005_00573" }, { "type": "cds", "sequence": "contig_1", "start": 319224, "stop": 320177, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "TTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTGGAAGTTTTATCATCAATGGTAGCGAAAAAGTTATCGTTAGTCAATTAATTAGATCACCTGGAGCTTACTTTGGACGTGGCGTTAGAAATAAACAATCTGATGACCTTTTCAACAAGGTAGAAATTTTACCTCGTATTGGTTCTTGGGTTGAAATCTCTCACAAGGTTACAACTAAAACACCAGATAGCGTAAAAATTAAAATCGATAAAAACAAAAATGTTAACTTAGTAACTTTCTTAGGTTCACTTGGTTTAGATCGCCAAATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGA", "aa": "MEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSGGSFIINGSEKVIVSQLIRSPGAYFGRGVRNKQSDDLFNKVEILPRIGSWVEISHKVTTKTPDSVKIKIDKNKNVNLVTFLGSLGLDRQMIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKK", "aa_hexdigest": "7fc93c917cd2c8d1eea12740016aa66a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 130, "aa_cov": 0.38801261829652994, "hmm_cov": 0.9230769230769231, "evalue": 2.1590202225432696e-07, "score": 27.07325553894043, "start": 108, "stop": 230 } ], "seq_stats": { "molecular_weight": 35709.0328, "isoelectric_point": 9.555520057678223 }, "id": "EFPKPENHBP_607", "locus": "GCA000186005_00574" }, { "type": "cds", "sequence": "contig_1", "start": 320178, "stop": 322136, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_Q98Q23", "UniRef:UniRef90_C4XEN6" ], "nt": "ATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGATCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGA", "aa": "MDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQ", "aa_hexdigest": "f4a833893f9c61d3741c0dda25f49b89", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028FF9953", "query_cov": 0.8634969325153374, "subject_cov": 0.9825479930191972, "identity": 0.51, "score": 563.0, "evalue": 1.63e-192, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A7V5ZEA3", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 319878, "stop": 322439, "observations": { "mutation": [ 320175, 322134, 322329 ], "stop": [ 320175, 322134, 322329 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN6", "query_cov": 1.0, "subject_cov": 0.5370675453047776, "identity": 1.0, "score": 1278.0, "evalue": 0.0, "gene_start": 1, "gene_end": 652, "reference_start": 411, "reference_end": 1062, "reference_sequence": "MMRKKEYYCMDVATKKYVNKYPYKVRKYGPITERRDYSVTKITWNVPDFLVTSKESFEWFKKTGIEESLREHYPITATNKKVSLEYIPNSADLEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSGWSFIINGSEKVIVSQLIRSPGAYFGRGVRNKQSDDLFNKVEILPRIGSWVEISHKVTTKTPDSVKIKIDKNKNVNLVTFLGSLGLDRQMIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKKWMDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQWLIDPITGNKLDKPVSVGVMYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMETWAIESYGATNVLQEILTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE" }, "paralog": false, "identity": 0.9964830011723329, "score": 1669.0, "evalue": 0.0, "description": "Internal stop codon at 320175, 322134, 322329. Nonsense mutation around 320175, 322134, 322329.", "psc": { "uniref90_id": "UniRef90_C4XEN6", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q98Q23", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN6", "EC:2.7.7.6", "UniRef:UniRef50_Q98Q23", "KEGG:K03043", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q23" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "EFPKPENHBP_608", "locus": "GCA000186005_00575" }, { "type": "cds", "sequence": "contig_1", "start": 322191, "stop": 322331, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79", "UniRef:UniRef90_A0A920JZ79" ], "nt": "ATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGA", "aa": "MYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMET", "aa_hexdigest": "6afa928b5687044909329d0b39d090c4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 322191, "stop": 322381, "observations": { "deletion": [ 322362 ], "mutation": [ 322329 ], "stop": [ 322329 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A920JZ79", "query_cov": 0.9782608695652174, "subject_cov": 0.703125, "identity": 0.8220000000000001, "score": 84.0, "evalue": 1.72e-20, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MYIIKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGATNVPSRSVDS" }, "paralog": false, "identity": 0.746031746031746, "score": 100.0, "evalue": 1.31e-29, "description": "Internal stop codon at 322329. Frameshift due to deletion around 322362. Nonsense mutation around 322329.", "psc": { "uniref90_id": "UniRef90_A0A920JZ79", "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A920JZ79", "UniRef:UniRef50_A0A920JZ79" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79" ], "product": "DNA-directed RNA polymerase" } }, "id": "EFPKPENHBP_609", "locus": "GCA000186005_00576" }, { "type": "cds", "sequence": "contig_1", "start": 322374, "stop": 322592, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "nt": "TTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAA", "aa": "MTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE", "aa_hexdigest": "43872484d4cbd0d223949c4907053db8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8L4", "query_cov": 0.9305555555555556, "subject_cov": 0.8933333333333333, "identity": 0.537, "score": 74.7, "evalue": 3.08e-16, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "uniref50_id": "UniRef50_A0A3B0P8L4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "product": "DNA-directed RNA polymerase subunit beta" }, "genes": [], "id": "EFPKPENHBP_610", "locus": "GCA000186005_00577" }, { "type": "cds", "sequence": "contig_1", "start": 322585, "stop": 322680, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGA", "aa": "MSNFIDKNKLNKDREIEKITLSLATKDDVLQ", "aa_hexdigest": "14a851522361cc5d273f42f8abbf6053", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3621.1214, "isoelectric_point": 6.026888465881346 }, "id": "EFPKPENHBP_611", "locus": "GCA000186005_00578" }, { "type": "cds", "sequence": "contig_1", "start": 323245, "stop": 323661, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGTCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGA", "aa": "MGKDYGIDFYEYNEIIHEYSDAIISTGSKAIEYLLEHVDLQKEAQLIEDEIKKINRSIKDKNHTGTRGQERSKLYKRLAVINAFINSGQDPKSMLIYDLPVIPADLRPLVQLDGGRHSTSDINELYRRIIIRNNRLKK", "aa_hexdigest": "c74c7a2c8f2075b913fff079790609c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 130, "aa_cov": 0.8623188405797102, "hmm_cov": 0.9615384615384616, "evalue": 1.2435571313233373e-22, "score": 76.9033432006836, "start": 20, "stop": 138 } ], "seq_stats": { "molecular_weight": 16003.04999999999, "isoelectric_point": 7.908546257019044 }, "id": "EFPKPENHBP_612", "locus": "GCA000186005_00579" }, { "type": "cds", "sequence": "contig_1", "start": 323680, "stop": 323958, "strand": "+", "frame": 1, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "ATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGACGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGA", "aa": "MLIKQNEYRMIQEAVDSLIDNARKKPNPVTSKDSRPLKSISDALTGKKGRFRQNLLGKRVDYSGRSVIVVGPELKMHQVGIPREMAAKLFEP", "aa_hexdigest": "cb37aaaa2b3672a596c9a0d2891c39d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030FE3613", "query_cov": 0.8913043478260869, "subject_cov": 0.9647058823529412, "identity": 0.6459999999999999, "score": 109.0, "evalue": 1.75e-29, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "EFPKPENHBP_613", "locus": "GCA000186005_00580" }, { "type": "cds", "sequence": "contig_1", "start": 324082, "stop": 324939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25", "PFAM:PF04983.23" ], "nt": "GTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGTAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAATGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGCGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGA", "aa": "MLLNRAPTLHRLSIQAYEPVLIRGKAIKLHPLSCTPFNADFDGDQMAVHVPISDEAVREAKELMLASRNILGPKDGEPIINPGQDIILGLYYLTQEATDDKVIGEGRYFGSYDEMMTAYENKLVSLHARVVLPISEINKRVIPADGQDRYIVSTVGKFIFNSIFPDSFEFIFGKYVERNIKEVDGKKVISEKEVLHTSGDKNQLKNYLLPYGMNFPEIIKNMPLNLPLGKKDIAKIVRRIFDRYVAIVTMEDLAAIISDLNSETINNLFDNCANLVDYNKNTIPV", "aa_hexdigest": "4491e408f55da7532f23bf48b0a78893", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 64, "aa_cov": 0.22456140350877193, "hmm_cov": 1.0, "evalue": 2.3721689352171308e-26, "score": 89.2982177734375, "start": 2, "stop": 65 }, { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 183, "aa_cov": 0.631578947368421, "hmm_cov": 0.73224043715847, "evalue": 5.811008502071216e-23, "score": 77.87889862060547, "start": 69, "stop": 248 } ], "seq_stats": { "molecular_weight": 32178.772000000023, "isoelectric_point": 5.638109779357909 }, "id": "EFPKPENHBP_614", "locus": "GCA000186005_00581" }, { "type": "cds", "sequence": "contig_1", "start": 325096, "stop": 325305, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGA", "aa": "MADVLDKLKALGYHYSTISGTTISISDVTTLPSTKDKIKEGDKYIEQLRDYFEQGLLTDDERYALTIQK", "aa_hexdigest": "748e7fd156653e0795e49424036d87ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7845.750899999997, "isoelectric_point": 4.898918724060058 }, "id": "EFPKPENHBP_615", "locus": "GCA000186005_00582" }, { "type": "cds", "sequence": "contig_1", "start": 325378, "stop": 326145, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCACGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTGTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGA", "aa": "MMFTSGARGNSSNFVQLAGMRGLMNNNTKILKADAENERVVRSTVEIPVKSSFLDGLTAYEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQGIVVREDDCGTDYGFEVKDILDTKTNTIIESLGERIEGRFSNVPIYHPRTKEMILDADQLITPQIAEEIVKAGIKSVEIRSVLSCYTKNGVCKKCYGKDLALNRVVNIGEAVGVVAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFGRLMELIDAYDSP", "aa_hexdigest": "b214c0c8d4d605aa3f50b5788d288fe7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20784313725490197, "hmm_cov": 0.9821428571428571, "evalue": 5.503067750202248e-12, "score": 41.91604232788086, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7647058823529411, "hmm_cov": 0.5948717948717949, "evalue": 1.4646957675694363e-46, "score": 155.34707641601562, "start": 56, "stop": 250 } ], "seq_stats": { "molecular_weight": 27669.165000000005, "isoelectric_point": 5.736384391784668 }, "id": "EFPKPENHBP_616", "locus": "GCA000186005_00583" }, { "type": "cds", "sequence": "contig_1", "start": 326425, "stop": 327060, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAA", "aa": "MKEVQRLYRLQGITISDKYIEIIIRQMLSKIVITDPGDSKFFSGNLVDIFAYQRESARLISQGKKPPFGEVKIKGAKQTPLLSDSFLAAASYQETPKILVNASIAAQSDKLEGLKENIILGHKIPAGTNCNFEEKGKYDIRDPRSYFQSKFAENSDFPNPNFEDNLNNLQSQLDEFNSVDPEELAIEEEMGIEEVYEDDYYSDLDENNDNN", "aa_hexdigest": "a21de706efa36cc86eee2f4bf5c11a92", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23991.430699999997, "isoelectric_point": 4.520939445495605 }, "id": "EFPKPENHBP_617", "locus": "GCA000186005_00584" }, { "type": "cds", "sequence": "contig_1", "start": 327727, "stop": 328197, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGA", "aa": "MKIKLKQTLIILSTTLVGTIALATGLSFLPTKDKTIPRGKQSFNDDHKNYNDPNIPNLPGNNVPTKNPKPTLVLKNRPKSANAITKENSIKYLALGDSISAGFNAQLDKDYPGQLVNSKIEGLSFPTYLASFFQQLADKKLESFDNKAVSGTTFKQ", "aa_hexdigest": "ff68db457853b9f942c62f73e6c18645", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17097.40229999998, "isoelectric_point": 9.726296806335448 }, "id": "EFPKPENHBP_618", "locus": "GCA000186005_00585" }, { "type": "cds", "sequence": "contig_1", "start": 328246, "stop": 328746, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGCGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGA", "aa": "MAELNKYFGTDLVNIKNDLIAKIKNANLITVTLGANDFLKDLLFKMVSNMPILDLIKQIKNKNLDYNMLVSHFDQVFRTLFEQLENRQKQFIQTIKSLNDKVNINFIAYPLPLTFIMDLIDKAINKGDTNNALSIGQTILDLLNKKLKYTAISQKTYYINPYQSSF", "aa_hexdigest": "24568c701aac3ed95bab71f0e0a0a1c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19174.1777, "isoelectric_point": 9.280111122131348 }, "id": "EFPKPENHBP_619", "locus": "GCA000186005_00586" }, { "type": "cds", "sequence": "contig_1", "start": 329125, "stop": 329226, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGA", "aa": "MFKSKIYETIDPNRKLEKFMHRDDYANQKSLRD", "aa_hexdigest": "d0f0b5888402d395d3ba5483dc28b33d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4118.6532, "isoelectric_point": 9.222669792175292 }, "id": "EFPKPENHBP_620", "locus": "GCA000186005_00587" }, { "type": "cds", "sequence": "contig_1", "start": 329506, "stop": 330051, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTTGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGA", "aa": "MLKLVKSFYNENISKYISQNDLASLLKLVLNTKSIKSAVGDLVVELINSAPEYAKCKSINELFNVFISNKNNQNTIKKFIESIVNDLLSQNEFKQIVLRLASKIIKQYPELFKDIDENNLTNLIYESLSLGGEINNALGLVTKFVNSLLNELTTTNIKEFKFDNVIKNTLTEIKTLFDKDN", "aa_hexdigest": "2c7e66bd216c2bf58613fee642f53b91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20601.528700000013, "isoelectric_point": 7.697386360168458 }, "id": "EFPKPENHBP_621", "locus": "GCA000186005_00588" }, { "type": "cds", "sequence": "contig_1", "start": 330283, "stop": 330666, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGA", "aa": "MSNLISQLVSDLLKTNQTQLMDVTNFFDAIKVIFKNFVNTSAYDYISPLIKKVFEFPEAKDLFKKAMAKIPAQEAKNLNDRDVADIVSFVLDNNEFKDILQDFLVKGIFGDAITIEKLKDANNIIKS", "aa_hexdigest": "26b8481b3cb62cff42b8bd457e13f4a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14425.519299999976, "isoelectric_point": 5.098309898376464 }, "id": "EFPKPENHBP_622", "locus": "GCA000186005_00589" }, { "type": "cds", "sequence": "contig_1", "start": 330826, "stop": 331014, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGA", "aa": "MENFIGDFLTIIPSLDEEFRFIKDVLTKIFNELKATGKAIDFKKVMSEFAKCIKNKLFNKND", "aa_hexdigest": "0255e34fa769216ded9bbcf6fc28bc14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7248.440299999999, "isoelectric_point": 7.8331182479858406 }, "id": "EFPKPENHBP_623", "locus": "GCA000186005_00590" }, { "type": "cds", "sequence": "contig_1", "start": 331063, "stop": 331965, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09204.15" ], "nt": "ATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGA", "aa": "MNKHQAFLRKFVKNILVYVSEDIALGKKLFKSLSEKAHKIIDKHIDENDFNNMFIDLFQRTEGLQRIIDNILDSLFSDIDSYQNANSLIDILKIYVMPQDRADRLAKNLEDVLKEFLKNEALHKLLRGLFEENVAPYGIDPKSPGNKALVDDLLANLHTFFVDLKILPNLANSMVDSFKKYNNFNEIIKNIEPILVNGLKLKEYSFVATILKNNIVNKHKESIRDDINKVIAGITSKINLVNKFIDDFNLIKPIKDMGLSETKARDFLTQVIQSQNLKTIIDVFVKELLDKNLEYAQLNS", "aa_hexdigest": "43ea93ea515c6d8e67721c724751e3c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09204.15", "name": "Colicin_immun", "length": 54, "aa_cov": 0.16, "hmm_cov": 1.0, "evalue": 1.8192244149478188e-06, "score": 24.79534912109375, "start": 37, "stop": 84 } ], "seq_stats": { "molecular_weight": 34552.618900000045, "isoelectric_point": 7.029585075378416 }, "id": "EFPKPENHBP_624", "locus": "GCA000186005_00591" }, { "type": "cds", "sequence": "contig_1", "start": 331987, "stop": 332103, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGA", "aa": "MSSPSALIIKGTIKQYITELFEKNEEVAFTIGKLIANG", "aa_hexdigest": "02765bf2b02be693f886f10db481de01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4155.807500000001, "isoelectric_point": 6.049339866638183 }, "id": "EFPKPENHBP_625", "locus": "GCA000186005_00592" }, { "type": "cds", "sequence": "contig_1", "start": 332230, "stop": 332676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGA", "aa": "MKRINTYGFEKLIDNLRDAAKEGALKFISEGGKVRLSKIFDQIPLIRDLLAKVPQKEFSDFLNLAFSSTPKNDTEGLFKVMFNSDKGTSKFEVGIGASGIIDILKGKVGDLVSAIASPLVKEFFKQASEQNYSDHNTLRKTNTGFQAL", "aa_hexdigest": "53a8667c8547f80e4d9fd11db7de92c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16379.613899999986, "isoelectric_point": 9.272439384460448 }, "id": "EFPKPENHBP_626", "locus": "GCA000186005_00593" }, { "type": "cds", "sequence": "contig_1", "start": 332749, "stop": 332922, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGCTGAAGCTTACTTAATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGA", "aa": "MTAEAYLMDGFVKAFKDNVKDYKTALLAKYGDKSLIGFNNSFDPLDYYLSGMTTVNY", "aa_hexdigest": "9a9d5de8cad1f81d6b402a827c8a7781", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6460.273900000002, "isoelectric_point": 4.919437599182127 }, "id": "EFPKPENHBP_627", "locus": "GCA000186005_00594" }, { "type": "cds", "sequence": "contig_1", "start": 333112, "stop": 333393, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGA", "aa": "MNKKEFKKRLIEYCETEGLSRYEENVAHALVKNINSKNFEVNYDNFGSLILHKKSKVKDAPKFMIAAHMDEVGYLVRQIHEDGQILLSSVGGI", "aa_hexdigest": "9358c8ae01ae0baa5ada4f3e8ff7af1a", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10670.157199999998, "isoelectric_point": 7.862451362609863 }, "id": "EFPKPENHBP_628", "locus": "GCA000186005_00595" }, { "type": "cds", "sequence": "contig_1", "start": 333484, "stop": 334197, "strand": "+", "frame": 1, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAA", "aa": "MEAEARNRAVTNKEIYADFGFKNKEEALKKNVQIGDRVLMSGETINFDNENLVGAKAMDNRAGVTTLEYIAHQLKDLDLAVDLYLVGTVQEEVGTRGAKTSVTVIDPQIAIALDTTSTHDTIGTIAGTTALGKGAAIRIMDGAMMANPRLVELMCKIGRKEKIKHYKFVAMGGGTDASALQYAKGGAATLTISLAQRYLHSPIGVCDIEDLMAAGDLIVETVKAINPKVYDQEIKYH", "aa_hexdigest": "2bf573c54280bf9338c038ebe2726a29", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8649789029535865, "subject_cov": 0.9715639810426541, "identity": 0.649, "score": 255.0, "evalue": 3.94e-83, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "EFPKPENHBP_629", "locus": "GCA000186005_00596" }, { "type": "cds", "sequence": "contig_1", "start": 334300, "stop": 334500, "strand": "+", "frame": 1, "gene": null, "product": "putative protein family (UPF0154)", "db_xrefs": [ "KEGG:K09976", "SO:0001217", "UniRef:UniRef50_Q98R64", "UniRef:UniRef90_A0A3P8KMY9" ], "nt": "ATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAA", "aa": "MAILFFLMGGFIAFIVSKKMFEKQIKENPPITEKMIRAMFLQMGRKASESQIKAVMRSMTNAKDEK", "aa_hexdigest": "6a8e6265ae53ebfde947e8b0bc752ba5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3P8KMY9", "query_cov": 0.9848484848484849, "subject_cov": 0.8125, "identity": 0.9229999999999999, "score": 114.0, "evalue": 7.78e-32, "valid": true, "product": "Uncharacterised protein family (UPF0154)", "uniref50_id": "UniRef50_Q98R64", "kegg_orthology_id": "K09976" }, "pscc": { "uniref50_id": "UniRef50_Q98R64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R64" ], "product": "UPF0154 protein MYPU_1460" }, "genes": [], "id": "EFPKPENHBP_630", "locus": "GCA000186005_00597" }, { "type": "cds", "sequence": "contig_1", "start": 334802, "stop": 335548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAGTTATCCTAGAACTGATAGTACTAGATTGAGTCAACATTTCTTAAATATTGCTAGAAGTTATATTAAAAATAAATTCGGTGAAGAATATATTCTTCAAGAAATCAAAGGCTTTGCAGGCGATCAAGATGCCCATGAAGCTATTAGACCAACTGATATAAACTTACTGCCTGAAGTTGCTAAAGCTAAATTTAATTTAGATAATAGTGAATACATTGTTTATAAGCTAATTTATGAACACACACTTATGTCTTTAATTAATCCACCAATTAAAGCTAATAAAACTTATACCTTTAAAAAAAATAAGTTAACTTTTAAAGCTAATGTAAGTAAAACCATATTCCCAGGTTACTATCTTGTTAAAAATGAAAATGAAGATATTGAAGATCCTAATTATCAAATACAACAAAAAGTGGATGTTTTAGAATATCAAATAACAAATCATGAAACTAAACCACCAGCTCGTTACACTGAAGGTTCATTAATTGAAGCTTTAGATAATATTAAAGTTGGACGTCCTTCAACTTTTGCAACTACTGTTGCAGTAAACTTGGAAAGAAAATATTTTGAAAATATTGATAAAAGTTTACATCCTACAGAATTTGGCCGTTTAGTTTTAAGTAAATTATTAGATGGTTTTCCAGATATTATTAATGAAGGCTATACAGCTGAAGTTGAAGAACAATTAGACTTAATTGCTTCAAATAAAACTACTGTTCAACCTATTATGTATGATTTTTGA", "aa": "MISYPRTDSTRLSQHFLNIARSYIKNKFGEEYILQEIKGFAGDQDAHEAIRPTDINLLPEVAKAKFNLDNSEYIVYKLIYEHTLMSLINPPIKANKTYTFKKNKLTFKANVSKTIFPGYYLVKNENEDIEDPNYQIQQKVDVLEYQITNHETKPPARYTEGSLIEALDNIKVGRPSTFATTVAVNLERKYFENIDKSLHPTEFGRLVLSKLLDGFPDIINEGYTAEVEEQLDLIASNKTTVQPIMYDF", "aa_hexdigest": "ff4d88f72bc9d24d9bff51216862bea4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28586.06860000002, "isoelectric_point": 5.490498924255371 }, "id": "EFPKPENHBP_631", "locus": "GCA000186005_00598" }, { "type": "cds", "sequence": "contig_1", "start": 335759, "stop": 335998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAGAATTATTGGTTTCAAATTAAGCCAATTAATGCAAAAGAAAATTTCAAATAGTCCTCGTACTCCAAGTGCAGGACGTGTACAATCAATTGCTCTTAAATTAGTAGTTGATCGTGAAGATGAAATTAATGCATTTGTGCCTCGTGGTTATCACAAAGTTGAAGCTAAATTAAATGAAACATTAAGTGCTTCTATTTATATAGATAAACATCCAGCAGCAGAAAAAAACTGA", "aa": "MLDRIIGFKLSQLMQKKISNSPRTPSAGRVQSIALKLVVDREDEINAFVPRGYHKVEAKLNETLSASIYIDKHPAAEKN", "aa_hexdigest": "3c96d2e6c57863e33521765f8e9558d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8877.174699999994, "isoelectric_point": 9.628240394592286 }, "id": "EFPKPENHBP_632", "locus": "GCA000186005_00599" }, { "type": "cds", "sequence": "contig_1", "start": 336284, "stop": 336439, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTAGTTATAGTGGAATCTCCAAACAAAGTTGAGACAATTAAAAAATATTTAGGAAGCGATTATGATGTGATTGCTTCTGTTGGTCATATCGCTATTATGAGTACTAGGAAAGGCGAACAAGGTTTAGGAATTGATTTAGAAAATTGA", "aa": "MNNLVIVESPNKVETIKKYLGSDYDVIASVGHIAIMSTRKGEQGLGIDLEN", "aa_hexdigest": "9faf956af32f225ce53d04ce4d2af35a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5561.301199999999, "isoelectric_point": 5.045847511291503 }, "id": "EFPKPENHBP_633", "locus": "GCA000186005_00600" }, { "type": "cds", "sequence": "contig_1", "start": 336803, "stop": 337045, "strand": "+", "frame": 2, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "SO:0001217", "UniRef:UniRef50_C4XEP2", "UniRef:UniRef90_C4XEP2" ], "nt": "ATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGA", "aa": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDI", "aa_hexdigest": "acdce77b6e5843fd69c6a76237c7d384", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 336803, "stop": 337276, "observations": { "mutation": [ 337043 ], "stop": [ 337043 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP2", "query_cov": 1.0, "subject_cov": 0.5095541401273885, "identity": 1.0, "score": 152.0, "evalue": 1.16e-45, "gene_start": 1, "gene_end": 80, "reference_start": 1, "reference_end": 80, "reference_sequence": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDIWRHLAINLDSERGYGKVKKESHRAKYEEKKEFKKSNKSAEACEKVAEEKAAKKPRSAKKESQEKEVKPRQSKAKKVE" }, "paralog": false, "identity": 0.9872611464968153, "score": 256.0, "evalue": 4.82e-89, "description": "Internal stop codon at 337043. Nonsense mutation around 337043.", "psc": { "uniref90_id": "UniRef90_C4XEP2", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_C4XEP2", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP2", "UniRef:UniRef50_C4XEP2", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP2" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "EFPKPENHBP_634", "locus": "GCA000186005_00601" }, { "type": "cds", "sequence": "contig_1", "start": 337304, "stop": 337453, "strand": "+", "frame": 2, "gene": null, "product": "Single strand binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7", "UniRef:UniRef90_A0A3P8MEY7" ], "nt": "ATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGA", "aa": "MNKVLLVGRISNDIRMYRTNSGISYARTSIAVNRRSSNDKITDFIPIVA", "aa_hexdigest": "624a0c9ac922e317e98f68c319adc8e4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 337304, "stop": 337479, "observations": { "deletion": [ 337439 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEY7", "query_cov": 0.9183673469387755, "subject_cov": 0.7758620689655172, "identity": 0.9109999999999999, "score": 85.1, "evalue": 5.86e-21, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MNKVLLVGRISNEIRMYRTNSGIAYARTSVAVNRRSSNDKITDFVSYCRMKKFSRISK" }, "paralog": false, "identity": 0.896551724137931, "score": 101.0, "evalue": 4.32e-30, "description": "Frameshift due to deletion around 337439.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEY7", "product": "Single strand binding protein", "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEY7", "UniRef:UniRef50_A0A3P8MEY7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7" ], "product": "Single strand binding protein" } }, "id": "EFPKPENHBP_635", "locus": "GCA000186005_00602" }, { "type": "cds", "sequence": "contig_1", "start": 337480, "stop": 337632, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGTTCTTTGTTCTCTTACTGAACGAGGTTCAAGTAATGAAATATTGTCAACTGTAACTTCATATGATCAAACTGTTCCATTTTGTCCTTCATATTGTGATGAAGTAAATGAACCTTCAATTGAAACAAGAGCTCCTTTTGAAGCATAG", "aa": "MPVLCSLTERGSSNEILSTVTSYDQTVPFCPSYCDEVNEPSIETRAPFEA", "aa_hexdigest": "b4499a9fb398002494902aaefd897972", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5515.054299999999, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_636", "locus": "GCA000186005_00603" }, { "type": "cds", "sequence": "contig_1", "start": 337855, "stop": 338109, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02963", "RefSeq:WP_013354516.1", "SO:0001217", "UniParc:UPI0001E3308C", "UniRef:UniRef100_C4XEP4", "UniRef:UniRef50_A5IY67", "UniRef:UniRef90_C4XEP4" ], "nt": "ATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAA", "aa": "MVFNNKKKNNFRRRRCETCDQKLTYVDYKDVEFLSKFITATGQIKPHSATGACAKDQRKIANAIKRARFMALIPYSKDRVRLVK", "aa_hexdigest": "bb4e0704f05956295c561802130d9245", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3308C", "ncbi_nrp_id": "WP_013354516.1", "uniref100_id": "UniRef100_C4XEP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3308C", "RefSeq:WP_013354516.1", "UniRef:UniRef100_C4XEP4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEP4", "uniref90_id": "UniRef90_C4XEP4" }, "psc": { "uniref90_id": "UniRef90_C4XEP4", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_A5IY67", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY67" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "EFPKPENHBP_637", "locus": "GCA000186005_00604" }, { "type": "cds", "sequence": "contig_1", "start": 338202, "stop": 338372, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGA", "aa": "MNFEKIFICTTDTVTGIGGPICQETLDALYYLKKRPKSKKIMILVASLKQAQSFKE", "aa_hexdigest": "b85bc97ab39d8c249103dfa7341fc723", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6340.497000000001, "isoelectric_point": 9.301772499084471 }, "id": "EFPKPENHBP_638", "locus": "GCA000186005_00605" }, { "type": "cds", "sequence": "contig_1", "start": 338451, "stop": 338648, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGA", "aa": "MPNNKKLLEFLEKNGPMYVTSANVSTQEPIDIKDAHKVFPEVKNVYNFGKGKGVASHIIDAQSNE", "aa_hexdigest": "21ce2223b919c584756e23f5caaa5add", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7232.124700000004, "isoelectric_point": 6.7218019485473635 }, "id": "EFPKPENHBP_639", "locus": "GCA000186005_00606" }, { "type": "cds", "sequence": "contig_1", "start": 338682, "stop": 339824, "strand": "-", "frame": 2, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6", "UniRef:UniRef90_C4XEP6" ], "nt": "ATGGAAAGCATTAAAGAAGGCAAGAAAATTTCACCTGACCAATTAATTGAAGAAGCTTTTAAATCAGATACAAATAAAATAGTTGAATCAATGGGTAAAGAATTAAAAGAAAAATATCCAAATTGTTTAGCTTTTTCGCCTATTGTTGTTGTCGAAGATATTACTAAAGATAAAGCTAAAGCTGTTGTTACAATTGTAATTTATCCAAAAGCTGAATTCGATAAAATTGACATAAATATTAAACCAGATGGTAAATATGAAATGGTGACAAAAGGTGCTATTGATGAAATTTACAAAAAATTTGTTGATGATTATCCTTTACTTAAAGAAGTTGAAGACCAATCAAAAGAAAATGATTATGTTAGATATAATATTTCAGTTTCTAAAGACAAAAAAGAAATATTGTCACGTAAAGATTTAGATAAAAAAATTGTAAAAGATGAAAATCCTAACTCAATTAATAGTCAATTAATGAATCGTAAAGTTAATGAATCATTTTCAGTAAATACACCAGAAAATGAAAACATTAAAATTACAGTTACTAAAGTTTTTCGTCCAATTAAAACTAAATTAACAAATGACAACATAAAAGAAATCAAAATTGAAGGCATTAATACTCTTGATGATCTTTATAATCAATTGTCAATGAATTTGAAAAATGAAATGGCTTCAAGTTATTTATTAGATTACATGAAAAAAGCAATTGAAACAGTTGGAAATAAATATGGTTTTGATTTACCACCTATTTTATTAGATACACAAGTAAATGATTTTATTAAAAGTAAATTAGCAATGTTACCTCCAGATAAAGCTAATCTATTAAGAAATGCAATAGAAAATAAACAAAAAGATGGTTATGAAATTTTTGATTTAGCAAAAACAAATGTTCGTAATTCTCTTTTAGACGCAATTATTGAATTTACTTTTTCATTGTTAATTAAAAACAAAATTACAGAAAAAGAAGCAGAAGAATTTTACAAATCAATTAAAGAAAAAACTTTAATTTCAACTGGCTCAAATGAATTAACAAAAGCTCAATCTGATGTTATTTTAGCTAAACAAAAAGCAGCTCTTAACTTGCTTTATCTTAATGATAAAGAAAGTTATGAAGCTGTTTCAAAAGATTTAGAATTAACTATTTAA", "aa": "MESIKEGKKISPDQLIEEAFKSDTNKIVESMGKELKEKYPNCLAFSPIVVVEDITKDKAKAVVTIVIYPKAEFDKIDINIKPDGKYEMVTKGAIDEIYKKFVDDYPLLKEVEDQSKENDYVRYNISVSKDKKEILSRKDLDKKIVKDENPNSINSQLMNRKVNESFSVNTPENENIKITVTKVFRPIKTKLTNDNIKEIKIEGINTLDDLYNQLSMNLKNEMASSYLLDYMKKAIETVGNKYGFDLPPILLDTQVNDFIKSKLAMLPPDKANLLRNAIENKQKDGYEIFDLAKTNVRNSLLDAIIEFTFSLLIKNKITEKEAEEFYKSIKEKTLISTGSNELTKAQSDVILAKQKAALNLLYLNDKESYEAVSKDLELTI", "aa_hexdigest": "b3cd4cf4494135e4138bc6e4039a8be5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEP6", "query_cov": 1.0, "subject_cov": 0.9223300970873787, "identity": 0.997, "score": 712.0, "evalue": 6.16e-258, "valid": true, "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP6" }, "pscc": { "uniref50_id": "UniRef50_C4XEP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6" ], "product": "Trigger factor" }, "genes": [], "id": "EFPKPENHBP_640", "locus": "GCA000186005_00607" }, { "type": "cds", "sequence": "contig_1", "start": 339930, "stop": 340268, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C", "UniRef:UniRef90_UPI0024C76683" ], "nt": "ATGAGAGGCTTCTTAGGAAGAATGTTATTTTCAAATGATTCAGTATTTAAAAATGTTAAAGTAACTTCAGGTGGAGAAAAAGCGAGATTGATGTTTTCAAGAATGATGCTTTTAGAATCTAACTTCTTGATTCTAGATCAACCTTTAGATCACCTTGATGCTGAAAGCATTGACAGTGTCATTGCAGGATTAAAAGCTTATAAGGGTGGTGTTATTGTAACAACATATAATAGAGCTTTAGTTAACCAAGTAGCTAATGTTATTGTTGAAATTGCTCCTGATAAAAGTTTTATTTATCATGGAACACTAGATGAATATGAAAAAATCATGAACTATTAA", "aa": "MRGFLGRMLFSNDSVFKNVKVTSGGEKARLMFSRMMLLESNFLILDQPLDHLDAESIDSVIAGLKAYKGGVIVTTYNRALVNQVANVIVEIAPDKSFIYHGTLDEYEKIMNY", "aa_hexdigest": "dce887d91e1c9a076258e30bafb102fa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 339936, "stop": 340568, "observations": { "mutation": [ 340319, 340331 ], "stop": [ 340319, 340331 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024C76683", "query_cov": 0.9910714285714286, "subject_cov": 0.4847161572052402, "identity": 0.8109999999999999, "score": 176.0, "evalue": 1.04e-53, "gene_start": 1, "gene_end": 110, "reference_start": 116, "reference_end": 226, "reference_sequence": "MNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKMVIVGEDDIAKTRLLECLYGIKKPTSGTIEWGQTITPSYFPNDNSKFFETDETILEWISKWPLENKEKVNQENDDARMRGFLGRMLFSNDSVFKKVNVTSGGEKFRLMFSRMMLLESNFLILDQPLDHLDTESIDSVIEGVNNYKGGAIFTTYNRAFVNQCANVILELQSPEKSFIFRGTLEEYEQIMLDE" }, "paralog": false, "identity": 0.6966824644549763, "score": 291.0, "evalue": 1.35e-101, "description": "Internal stop codon at 340319, 340331. Nonsense mutation around 340319, 340331.", "psc": { "uniref90_id": "UniRef90_UPI0024C76683", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024C76683", "UniRef:UniRef50_UPI0030841F7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "EFPKPENHBP_641", "locus": "GCA000186005_00608" }, { "type": "cds", "sequence": "contig_1", "start": 340329, "stop": 340571, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGACTTATGTTGATGAAAAAGGAAATACACTATTCCAAAATGTTTCATTCACTTTAGCTCGTGGTGAAAAAATGGTTTTAATTGGTGAAGATGATATTGCTAAAACTAGATTTTTAGAATGCTTAATTGGTAAGATAAAACCAACAAAAGGCACTATAAAATGGGGCCAAACAATAAAACACACTTATTATCCAAATGAAAATAAAGAATATTTTAAAGAAGATATAACAATTCTAGAATGA", "aa": "MTYVDEKGNTLFQNVSFTLARGEKMVLIGEDDIAKTRFLECLIGKIKPTKGTIKWGQTIKHTYYPNENKEYFKEDITILE", "aa_hexdigest": "fb132871bd886214785d80abd84b6037", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.7, "hmm_cov": 0.9122807017543859, "evalue": 7.493164333862914e-09, "score": 32.64236068725586, "start": 13, "stop": 68 } ], "seq_stats": { "molecular_weight": 9305.617999999995, "isoelectric_point": 6.581352806091309 }, "id": "EFPKPENHBP_642", "locus": "GCA000186005_00609" }, { "type": "cds", "sequence": "contig_1", "start": 340617, "stop": 340793, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3", "UniRef:UniRef90_UPI0021FF81A7", "PFAM:PF12848.13" ], "nt": "ATGAAACAATCAAATGCTAAAAAAGAAGTACAAATTGAAAAACTTAAAGCATTTATTGCTCGTTTTAGCGCCAACGCTTCTAAAAGTAAACAAGCAACCAGTAGAAAAAAATCTTTAGAAAAAATTACTCTTGATGAAATTAAACCTTCTAATCGTAAATATCCTTATATTAATTGA", "aa": "MKQSNAKKEVQIEKLKAFIARFSANASKSKQATSRKKSLEKITLDEIKPSNRKYPYIN", "aa_hexdigest": "bf4955c82b17bcc239a7c115be587dbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 340497, "stop": 340850, "observations": { "mutation": [ 340619, 340826 ], "stop": [ 340619, 340826 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF81A7", "query_cov": 0.9827586206896551, "subject_cov": 0.4830508474576271, "identity": 0.877, "score": 94.0, "evalue": 1.36e-23, "gene_start": 1, "gene_end": 57, "reference_start": 20, "reference_end": 76, "reference_sequence": "MYTGNYSFWKQSSELARELMKQSNVKKEAQMEKLKEFIARFSANASKSKQYTSRKKALEKITLDEIKPSNRKYPFIRWDMNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKW" }, "paralog": false, "identity": 0.7606837606837606, "score": 176.0, "evalue": 1.51e-58, "description": "Internal stop codon at 340619, 340826. Nonsense mutation around 340619, 340826.", "psc": { "uniref90_id": "UniRef90_UPI0021FF81A7", "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF81A7", "UniRef:UniRef50_UPI00220019C3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3" ] } }, "hypothetical": true, "pfams": [ { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 54, "aa_cov": 0.9310344827586207, "hmm_cov": 0.9629629629629629, "evalue": 4.148002058411154e-14, "score": 48.78986740112305, "start": 3, "stop": 56 } ], "seq_stats": { "molecular_weight": 6685.7099, "isoelectric_point": 10.248297309875486 }, "id": "EFPKPENHBP_643", "locus": "GCA000186005_00610" }, { "type": "cds", "sequence": "contig_1", "start": 340965, "stop": 341087, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGTCAGAATTAACTGCTAATCAAAAAATTAAAGTTTTATTAGCCAAAGCATTATTTGGTAATCCTGATATTTTAATCATGGACGAACCTACTAACAATCTTGACTTGCGTGCAATTAAATGA", "aa": "MSELTANQKIKVLLAKALFGNPDILIMDEPTNNLDLRAIK", "aa_hexdigest": "c3f1ca6a81b111cbf9e4bb5c858949f1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 31, "aa_cov": 0.775, "hmm_cov": 1.0, "evalue": 1.4903140628746068e-06, "score": 25.197847366333008, "start": 2, "stop": 32 } ], "seq_stats": { "molecular_weight": 4453.2288, "isoelectric_point": 6.0008562088012685 }, "id": "EFPKPENHBP_644", "locus": "GCA000186005_00611" }, { "type": "cds", "sequence": "contig_1", "start": 341157, "stop": 341543, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "nt": "ATGATAGAAGTAAATAATCTAAGTAAAATTTTTAGCGACAAAAAATTATTTGAAAATGTTAATTTAAAATTTACAGCTGGAAATACATATGGAATAATTGGAGCCAATGGAGCTGGCAAAAGTACATTTTTAAAAATTTTAGCTGGTGAAATAGAAGCTACCGGCGGCCAAATAATAATTGAAAAAAGCCGAAGACTTAGTGTTTTAAGCCAAGATCATAATGCTTATGACAATATGAATGTAACCGATGTTGTAGTTATGGGAAATACTGATTTATATAAAATCAAACAAGAAAAAGATGCTATTTATGCAAATCCTGAAGCAACAATGGAAGACTATACAAGGGCAGGAGAATTAGAAGAAAAATTCGGCGAATTAGGTGGTTGA", "aa": "MIEVNNLSKIFSDKKLFENVNLKFTAGNTYGIIGANGAGKSTFLKILAGEIEATGGQIIIEKSRRLSVLSQDHNAYDNMNVTDVVVMGNTDLYKIKQEKDAIYANPEATMEDYTRAGELEEKFGELGG", "aa_hexdigest": "f387d22bab52c37a23d576cc4bd3c611", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002614D0AA", "query_cov": 1.0, "subject_cov": 0.9343065693430657, "identity": 0.57, "score": 138.0, "evalue": 9.1e-40, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002614D0AA" }, "pscc": { "uniref50_id": "UniRef50_UPI002614D0AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_645", "locus": "GCA000186005_00612" }, { "type": "cds", "sequence": "contig_1", "start": 341626, "stop": 342216, "strand": "-", "frame": 1, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGCCACTAACCAATCAAAATATTTATTTTCTTAAAATACCTTATGTTACAAATCTAATTACTTTAAAAAACTTTTTCTTTTCAATGACTAAAAAACAAAATGCCATTAAAGAAGTTATGAGATATTTTGACAGCTTAATTAGTGAATTAGAAAATACTAATACAATTATTATTCCTGATGAATTTATAAATAGCAAAATATCAGATTATACTCATCAATATTTAGAAAAAGTAAATCCAAATAATCGAGCAGCTATTGAAAAAAGTATTCAAGAAAAAGATCAATATGGTAAACAAATAATAGATAATGCGATTAATAGTGCTAGAAGAGAAATTGTTGAAGACATTGTTAAAAAATTAGTTTACACATTAGAAAAAATAAAAGTCCATGACACAGAAGTAAATCAAGAATTAAATCTAAGCGAAAATATTTTATTTCAATCAAACAACTATTACAATAGAAATGAAAACAAAAATGTTAACACTGTAAAAGAAGATTTAATTAATATAAAAATAGCTTTAAAACTTTTACAACTAAATGCACCTCAATTATTTTTAGTAATTGCTAGAGATGCAGCTATTGCAGTATAG", "aa": "MPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV", "aa_hexdigest": "6337901f89b1b553956cb07860501614", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 341626, "stop": 342516, "observations": { "mutation": [ 342255 ], "stop": [ 342255 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 366.0, "evalue": 2.11e-124, "gene_start": 1, "gene_end": 196, "reference_start": 225, "reference_end": 420, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.9966216216216216, "score": 577.0, "evalue": 9.58e-210, "description": "Internal stop codon at 342255. Nonsense mutation around 342255.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "EFPKPENHBP_646", "locus": "GCA000186005_00613" }, { "type": "cds", "sequence": "contig_1", "start": 342253, "stop": 342858, "strand": "-", "frame": 1, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGATCAATTATTTATTAAAAAGAGAAAATATTAAAATGAATAAAGATGAAATTGAGGCAATGATAAATAATACTGTTAGAGATTTAAAAATTGACAGAACTAAAATTGATTCAATGTCTTTATTAGAAAAATCTTTTGATTATAAATGCAAAAAAATATATAACTTAATTTCAAATGAAATTTTAAAAAGTGATGCTTTAGCCTATATTGCTAGTCCAGCAATTAGAAAAGATATTATTTCAAATAACACTATTAGTGCGGAAATTGTGGTTGCTTATTATCCTTTTGAAGAGCTTAAAAAAATTAACTATAAAAATACTCCAGTAAATTATATTTACAACTTTTTTGATGAAAATTCAATTGGTGCAATTTTTGAAAATACAATTAAAAATTACACATTTAAAAATTCAGTAAATGATTTTATTAAACAAAACGACTTAGTTAAGTTAAACATTAAATTATTTCAAAATAATATCTTAATGAAAGATGATAAAAATGTTCTTATAATAGCTAACCACACAAACAACTTTACTATTAATAATTCAATTCTTAATCATAGATATGGTGAAATTTTTAGCATCAATGATCCTTTAGGTAGAAAATGA", "aa": "MINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRK", "aa_hexdigest": "0a93f1f061d39da5c95b12f4aa85f321", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 341950, "stop": 342888, "observations": { "mutation": [ 342255, 342873 ], "stop": [ 342255, 342873 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4785714285714286, "identity": 1.0, "score": 389.0, "evalue": 2.86e-133, "gene_start": 1, "gene_end": 201, "reference_start": 11, "reference_end": 211, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.9903846153846154, "score": 610.0, "evalue": 6.76e-223, "description": "Internal stop codon at 342255, 342873. Nonsense mutation around 342255, 342873.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "EFPKPENHBP_647", "locus": "GCA000186005_00614" }, { "type": "tRNA", "sequence": "contig_1", "start": 343156, "stop": 343230, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 80.8, "nt": "GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 343188, 343190 ], "id": "EFPKPENHBP_8", "locus": "GCA000186005_00615" }, { "type": "tRNA", "sequence": "contig_1", "start": 343234, "stop": 343309, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.4, "nt": "GGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 343267, 343269 ], "id": "EFPKPENHBP_9", "locus": "GCA000186005_00616" }, { "type": "tRNA", "sequence": "contig_1", "start": 343314, "stop": 343389, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 79.7, "nt": "GGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 343347, 343349 ], "id": "EFPKPENHBP_10", "locus": "GCA000186005_00617" }, { "type": "tRNA", "sequence": "contig_1", "start": 343391, "stop": 343466, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 91.8, "nt": "GCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 343424, 343426 ], "id": "EFPKPENHBP_11", "locus": "GCA000186005_00618" }, { "type": "tRNA", "sequence": "contig_1", "start": 343558, "stop": 343642, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 59.4, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 343586, 343588 ], "id": "EFPKPENHBP_12", "locus": "GCA000186005_00619" }, { "type": "cds", "sequence": "contig_1", "start": 343804, "stop": 343941, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGATAATTAATTTTAAAATGTTTGAAAATGCAGCAATTAGACACAACTTGAAAATTTTGCAAGTTGATTATTTTGAAAAAAATATAACAATTGATATTTTTTGTGGCAACAATTCTTATATTAAATGA", "aa": "MKNKIINFKMFENAAIRHNLKILQVDYFEKNITIDIFCGNNSYIK", "aa_hexdigest": "babf2593f658da377b49c0a53648ce13", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.2961, "isoelectric_point": 9.302159309387207 }, "id": "EFPKPENHBP_648", "locus": "GCA000186005_00620" }, { "type": "cds", "sequence": "contig_1", "start": 344090, "stop": 344254, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01330.27" ], "nt": "ATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGA", "aa": "MVLYRIGEIVYKNNQNLIFESQGVGYSIIFPDHARVEAKQKMKLYLYEIRNDYT", "aa_hexdigest": "48038776229e059bd268cfe29ed1aa7b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01330.27", "name": "RuvA_N", "length": 51, "aa_cov": 0.9074074074074074, "hmm_cov": 1.0, "evalue": 9.138204794707335e-08, "score": 28.46644401550293, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 6473.391900000002, "isoelectric_point": 8.005506706237792 }, "id": "EFPKPENHBP_649", "locus": "GCA000186005_00621" }, { "type": "cds", "sequence": "contig_1", "start": 344555, "stop": 344692, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAG", "aa": "MLGFKAAQIDSALTKVELNQDVESMIEEAIKFISQSGAQNEQLNA", "aa_hexdigest": "7e7e499e2935b65702a950444eae9e99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 35, "aa_cov": 0.7333333333333333, "hmm_cov": 1.0, "evalue": 3.140767101820881e-08, "score": 30.333208084106445, "start": 2, "stop": 34 } ], "seq_stats": { "molecular_weight": 4911.4763, "isoelectric_point": 4.250954246520997 }, "id": "EFPKPENHBP_650", "locus": "GCA000186005_00622" }, { "type": "cds", "sequence": "contig_1", "start": 344685, "stop": 345635, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "SO:0001217", "UniRef:UniRef50_Q4A6N6", "UniRef:UniRef90_A0A3P8KA19" ], "nt": "ATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGATATCATCTTTATAGATGAAATTCATAGCATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAG", "aa": "MLRPANFFEFVGQEKLIYTLKTMIEGSRYRKEPLDHILFYGPPGTGKTSLASILANELDTKIHYLQGALLEKKSDILTVFANVKENDIIFIDEIHSMNKNVEEIIYNAMEDYKIDIIIGPEGNSKVMRMNLKPFTLIGATTKINLLTQPLKDRFGFIARLSQYNEKEIAKILENSEMVLNIKSERGVIPLIAKYSKGTPRVANHLLKRILDFSIQNKEDEITKKTTYSTFKHLELYDLGLGREHIEYLKVLNETFENKFAAIDSIAGVLNIDRDILIYELEPLLLYLKLIAKGPRGRKITTKGIDYLLKNKLDIHY", "aa_hexdigest": "338f66fcfd1b8885ba5e1fdd2cbede44", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KA19", "query_cov": 1.0, "subject_cov": 0.9875, "identity": 1.0, "score": 623.0, "evalue": 2.4e-225, "valid": true, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q4A6N6", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N6" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "EFPKPENHBP_651", "locus": "GCA000186005_00623" }, { "type": "cds", "sequence": "contig_1", "start": 345838, "stop": 346299, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGA", "aa": "MSIEYGGGVEILVQAQKNGKKVNDKYAKNIANTMDDRLTGGIAFNGTTVNVEGDGILKITQNGKLSSKEKEELEKIVSSKPNLILTDGDMIPLFVRGKFNESREKIDYSRIEDYIPPLKENGAKFKFIPPSEKGRSGYYGIDVSLRNNAAADE", "aa_hexdigest": "4667b0b884826d93bf19f3a9421556b0", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16875.891599999984, "isoelectric_point": 6.404697227478027 }, "id": "EFPKPENHBP_652", "locus": "GCA000186005_00624" }, { "type": "cds", "sequence": "contig_1", "start": 346561, "stop": 347202, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02355.23" ], "nt": "TTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGA", "aa": "MKGTQFFISGRFNEAQAKKTAANINFGSANYDLKILSSNYIEASKTSNSFRSALLAGAVVFSIIAVFMIVNYGLLGALSTISIALYMFLTLLLFTVLRGEYSPVTIAALIIGIGISVDSNIITFERLKNEVYAGDRLKKAMKNANHRTLGSILDANILTLLISFVLFFFGTSTIKGFSISLVFSVTFILIVMLIFTRGLSVLLVNTGAFDKKL", "aa_hexdigest": "2a71420e1fa4ac53e07a7acd7d525e25", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02355.23", "name": "SecD_SecF_C", "length": 161, "aa_cov": 0.7417840375586855, "hmm_cov": 1.0, "evalue": 5.156306017730186e-15, "score": 51.66889190673828, "start": 30, "stop": 187 } ], "seq_stats": { "molecular_weight": 23212.17610000001, "isoelectric_point": 9.897847175598145 }, "id": "EFPKPENHBP_653", "locus": "GCA000186005_00625" }, { "type": "cds", "sequence": "contig_1", "start": 347389, "stop": 347499, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGA", "aa": "MEFGGGVRISIESANSAYVNSDILDIQNKFMAHMNK", "aa_hexdigest": "bc270f5c0f2dd1d01024fd82a607eb59", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3988.4829, "isoelectric_point": 5.4648645401000975 }, "id": "EFPKPENHBP_654", "locus": "GCA000186005_00626" }, { "type": "cds", "sequence": "contig_1", "start": 347539, "stop": 347775, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGA", "aa": "MDLGNGNFKLQIETSQDLSNVLSKIESDVATFVSSGNKPRFIAYAISTSRAFDLLKYSMIAIAGAFLVVIVYTFIRFK", "aa_hexdigest": "0ca290aeffc772b4f154dd0e124788c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8643.935499999996, "isoelectric_point": 9.156267356872558 }, "id": "EFPKPENHBP_655", "locus": "GCA000186005_00627" }, { "type": "cds", "sequence": "contig_1", "start": 347821, "stop": 348174, "strand": "+", "frame": 1, "gene": null, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "nt": "ATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGA", "aa": "MSIAFIVVARLQLSPIMIAALLSITAFSINDTIIVFERIKETINTQYHGVFLSKALIKEIANNAIKDTFKRSLYTTLTTVVSILVLLMFGNATDLSFNIVMIFGLVVGSYSSIFICT", "aa_hexdigest": "b62689f9cb1c4fc95ff0771e77b94d8f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9G7", "query_cov": 0.9743589743589743, "subject_cov": 0.9193548387096774, "identity": 0.579, "score": 109.0, "evalue": 9.97e-29, "valid": false, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A432F9G7" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9G7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_656", "locus": "GCA000186005_00628" }, { "type": "cds", "sequence": "contig_1", "start": 348402, "stop": 348707, "strand": "+", "frame": 3, "gene": "truB", "product": "TruB family pseudouridylate synthase (N terminal domain)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1", "UniRef:UniRef90_UPI000FF45FC1" ], "nt": "ATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGA", "aa": "MFYLIKKTKGISSFSCIKTFAKKMNIKKIGHSGTLDPLASGLLLCASEDDTKLIPYISHKSKTYISKIIFGKHTSTYDSEGEITNTSEVKIKEEDLEKINK", "aa_hexdigest": "22f91c7a57129b1d5b2754c594a64c1a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF45FC1", "query_cov": 1.0, "subject_cov": 0.8859649122807017, "identity": 1.0, "score": 197.0, "evalue": 5.24e-64, "valid": true, "product": "TruB family pseudouridylate synthase (N terminal domain)", "uniref50_id": "UniRef50_UPI000FF45FC1" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF45FC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1" ] }, "genes": [ "truB" ], "id": "EFPKPENHBP_657", "locus": "GCA000186005_00629" }, { "type": "cds", "sequence": "contig_1", "start": 348960, "stop": 349253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTAATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAA", "aa": "MSELERIKIGKLALSNTKNEYEQIEINNLFDLDFYNATDSEIESLKKGQVINANKKLKDNKYLLINKTKDQNLILGILELKNNNLKVVKLFGNRLEE", "aa_hexdigest": "6f7e1c7ec8dc8dd5f9e3cb8b3a390db3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11254.82969999999, "isoelectric_point": 7.867995643615723 }, "id": "EFPKPENHBP_658", "locus": "GCA000186005_00630" }, { "type": "cds", "sequence": "contig_1", "start": 349256, "stop": 349624, "strand": "+", "frame": 2, "gene": null, "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7", "UniRef:UniRef90_A0A3P8LIG5" ], "nt": "ATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGA", "aa": "MKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYG", "aa_hexdigest": "f68b02b222dbfe34258929425b1c9a06", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 349226, "stop": 349927, "observations": { "mutation": [ 349622, 349652, 349829, 349886 ], "stop": [ 349622, 349652, 349829, 349886 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LIG5", "query_cov": 1.0, "subject_cov": 0.4206896551724138, "identity": 1.0, "score": 245.0, "evalue": 5.03e-80, "gene_start": 1, "gene_end": 122, "reference_start": 11, "reference_end": 132, "reference_sequence": "MIWQPIRGVIMKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYGWYSPKMNINTWFLSPHQAKMKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQWATGVFVSPKGITKSHTLDWLAKYLKLDSKKNLIAFGDSSNDYEMIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK" }, "paralog": false, "identity": 0.9785407725321889, "score": 455.0, "evalue": 5.97e-165, "description": "Internal stop codon at 349622, 349652, 349829, 349886. Nonsense mutation around 349622, 349652, 349829, 349886.", "psc": { "uniref90_id": "UniRef90_A0A3P8LIG5", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LIG5", "UniRef:UniRef50_A0A449B5A7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7" ], "product": "COF family HAD hydrolase protein" } }, "id": "EFPKPENHBP_659", "locus": "GCA000186005_00631" }, { "type": "cds", "sequence": "contig_1", "start": 349679, "stop": 349831, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGA", "aa": "MKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQ", "aa_hexdigest": "19fc09a76ae14d87ebb826c9a48e764f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5734.4913, "isoelectric_point": 5.507266426086424 }, "id": "EFPKPENHBP_660", "locus": "GCA000186005_00632" }, { "type": "cds", "sequence": "contig_1", "start": 349961, "stop": 350098, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAA", "aa": "MIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK", "aa_hexdigest": "feb24920720995d9d47f54d63584c650", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4965.651199999998, "isoelectric_point": 4.716181373596191 }, "id": "EFPKPENHBP_661", "locus": "GCA000186005_00633" }, { "type": "cds", "sequence": "contig_1", "start": 350118, "stop": 350990, "strand": "+", "frame": 3, "gene": null, "product": "FAD synthase", "db_xrefs": [ "RefSeq:WP_013354548.1", "SO:0001217", "UniParc:UPI0001E330A9", "UniRef:UniRef100_C4XEQ5", "UniRef:UniRef50_C4XEQ5", "UniRef:UniRef90_C4XEQ5" ], "nt": "ATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAG", "aa": "MSLKVYTFDNIPKFQKPIFIIGSFESFHLGHYKLLEKARELAKESEKERDIVLVYFEDIENLSKTKNLIFSDAKNRVQEFANLKINFAISLFYSQISHLSAQDFIDKLIANQDDYSFVIGEEFKFGFKRSGNVQFLENHYNKDKVFAQEVVRLKNKQKISTSYLKELIEFGDIDLLNTINMFPYSFSAKFSTIENKIELKKHELLIPLRQGIYAVLVELNNYNYYALMQVNFDTKYFIEFLDFKIKENQNIDARIKVLKAIRFFRNKELESINEQNKLNAKSILLDLNNN", "aa_hexdigest": "8be05d7590d4d50ccd4ba131fd99ec20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330A9", "ncbi_nrp_id": "WP_013354548.1", "uniref100_id": "UniRef100_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330A9", "RefSeq:WP_013354548.1", "UniRef:UniRef100_C4XEQ5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEQ5", "uniref90_id": "UniRef90_C4XEQ5" }, "psc": { "uniref90_id": "UniRef90_C4XEQ5", "product": "FAD synthase", "uniref50_id": "UniRef50_C4XEQ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ5" ], "product": "FAD synthase" }, "genes": [], "id": "EFPKPENHBP_662", "locus": "GCA000186005_00634" }, { "type": "cds", "sequence": "contig_1", "start": 351205, "stop": 351795, "strand": "+", "frame": 1, "gene": null, "product": "nucleotidyltransferase", "db_xrefs": [ "EC:6.3.1.22", "KEGG:K27343", "SO:0001217", "UniRef:UniRef50_C4XEQ6", "UniRef:UniRef90_C4XEQ6" ], "nt": "ATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGAGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGA", "aa": "MSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDIRAYSPMTFKKVPRTTASLY", "aa_hexdigest": "03e392d826bce26bb90ca861f94cc335", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 350974, "stop": 351986, "observations": { "insertion": [ 351919, 351950 ], "mutation": [ 351163, 351793 ], "stop": [ 351163, 351793 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEQ6", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 384.0, "evalue": 1.29e-132, "gene_start": 1, "gene_end": 196, "reference_start": 78, "reference_end": 273, "reference_sequence": "MLIIISKKTNYFYFALLFASKINIWFFIFIYYNNKYLFKRRTMAVGIIAEYNPFHNGHIKQINWVKEHFPGEKIVVVMSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDIRAYSPMTFKKVPRTTASLYWKFQKIMNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVKHVLQKDIQLKNPKNNSLNFK" }, "paralog": false, "identity": 0.9910714285714286, "score": 650.0, "evalue": 5.15e-240, "description": "Internal stop codon at 351163, 351793. Frameshift due to insertion around 351919, 351950. Nonsense mutation around 351163, 351793.", "psc": { "uniref90_id": "UniRef90_C4XEQ6", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_C4XEQ6", "kegg_orthology_id": "K27343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEQ6", "EC:6.3.1.22", "UniRef:UniRef50_C4XEQ6", "KEGG:K27343" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ6" ], "product": "tRNA(Met) cytidine acetate ligase" } }, "id": "EFPKPENHBP_663", "locus": "GCA000186005_00635" }, { "type": "cds", "sequence": "contig_1", "start": 351811, "stop": 351975, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTTGAAGCATGTACTTCAAAAAGATATACAGCTTCAAGAATCCAAAGAATAATAGCTTGA", "aa": "MNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVEACTSKRYTASRIQRIIA", "aa_hexdigest": "dac9956a6a16b0839c58984e796d96f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6269.3001, "isoelectric_point": 9.917961311340331 }, "id": "EFPKPENHBP_664", "locus": "GCA000186005_00636" }, { "type": "cds", "sequence": "contig_1", "start": 352166, "stop": 352573, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGA", "aa": "MDSTNKKKILEVQGLKKYFFNNNKINKAVDNVSFNLYEGQILGLIGESGSGKTTVGRSIAKLYDDFQGLLLVDGKLINNKKMSESDRKFLRKNIQMIFQNPKNSLDEQKSIYSILKESLIANNILKIKTKEIFKN", "aa_hexdigest": "5e0d2e4b6d055a4df5b2ba9abe5ff329", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9185185185185185, "subject_cov": 0.96875, "identity": 0.621, "score": 147.0, "evalue": 4.09e-43, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_665", "locus": "GCA000186005_00637" }, { "type": "cds", "sequence": "contig_1", "start": 352823, "stop": 353020, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGA", "aa": "MLEFYEQKQIDYTNGNFPFGIFDFIELENERKKALILTKMSKENYESMQLIDNLNNEMKNIKKEF", "aa_hexdigest": "d14b506dad54c79ae4b3d9f26d882cc5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.971699999996, "isoelectric_point": 4.8526517868041985 }, "id": "EFPKPENHBP_666", "locus": "GCA000186005_00638" }, { "type": "cds", "sequence": "contig_1", "start": 353102, "stop": 353713, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGA", "aa": "MNNTLDEELYKFDYKKAFLAKMQIDLLNKNKKLINNLLVKDIKELIYDLKNYNLDFFNKYFQPLEFNKKIKENMQSTYETYYEFDFNKYIIRSNLNLTQYEEKIDHIKKLISKQMILSKNAGDVKNSIEYLNELKTKIEKIDLEFEDKLVNHKEETLKKVKQVETELDEKEKINNVLKIRQSNFYQNYSDIKLLKLFNTFLAN", "aa_hexdigest": "67a7453ca611bcae005df33fb02c1ac2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24647.12770000004, "isoelectric_point": 8.653800773620606 }, "id": "EFPKPENHBP_667", "locus": "GCA000186005_00639" }, { "type": "cds", "sequence": "contig_1", "start": 353864, "stop": 354496, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "ATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAATTGAGCCAAATCACTATTTATATGGAACTAGAAATCAAGTGCTCAAGTGA", "aa": "MGLSHKNSKSFFGVFKDFFTNQKIKRNVKELLCKNIIYKALEDVGLLKQFAYRYPHEFSGGQLQRVAIARALIVEPKIIIADEPIASLDISIQAQVVNLLKELCEKRNIGVIFIAHDLSMIEYLADEVQIMHHGKFVEGGKTNIIYKKPMHPYTQNLFASIPRVENANKKFINQDFEAKYLEEQKFSNTTDLYLIEPNHYLYGTRNQVLK", "aa_hexdigest": "10e973adcf75266caea3e3530c76d264", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 0.9333333333333333, "subject_cov": 0.9468599033816425, "identity": 0.633, "score": 246.0, "evalue": 7.85e-80, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_668", "locus": "GCA000186005_00640" }, { "type": "cds", "sequence": "contig_1", "start": 354946, "stop": 355143, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTAGATACAATTAGTTCACTTGCTTTATTAACTCCAGTTTTGGCTGCTAGTTGTAAGCCAATTTTAGTTGTTAAAGAAGAATATAACAAGGAAAAAAGTTATCTTAAAACATTTCTAAAAACTATTAATAGCAAAAGCGATCTAGAAAAAAATGAATTCAAAAAATTTAGAATTAATTTTCATACTTTTTAA", "aa": "MILDTISSLALLTPVLAASCKPILVVKEEYNKEKSYLKTFLKTINSKSDLEKNEFKKFRINFHTF", "aa_hexdigest": "106acd6d13e317bcbc94095e529e3111", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7538.819599999997, "isoelectric_point": 9.374750709533693 }, "id": "EFPKPENHBP_669", "locus": "GCA000186005_00641" }, { "type": "cds", "sequence": "contig_1", "start": 355345, "stop": 355641, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7", "UniRef:UniRef90_UPI000FEECEC7" ], "nt": "TTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGA", "aa": "MPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQE", "aa_hexdigest": "c9c5ba0b2045cbcfddbd7c7a8d9c4e8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 355258, "stop": 355773, "observations": { "mutation": [ 355267, 355639 ], "stop": [ 355267, 355639 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECEC7", "query_cov": 1.0, "subject_cov": 0.5664739884393064, "identity": 0.99, "score": 176.0, "evalue": 7.38e-55, "gene_start": 1, "gene_end": 98, "reference_start": 30, "reference_end": 127, "reference_sequence": "MPIWGQILLVLFSSIFLSTIFVVLTYKFFLPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQEWIESNKGYEEEFTFYNKAYEYQIDFFDFDLKSAKYMVVESNNLLRL" }, "paralog": false, "identity": 0.9824561403508771, "score": 325.0, "evalue": 4.98e-116, "description": "Internal stop codon at 355267, 355639. Nonsense mutation around 355267, 355639.", "psc": { "uniref90_id": "UniRef90_UPI000FEECEC7", "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECEC7", "UniRef:UniRef50_UPI000FEECEC7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11468.611399999989, "isoelectric_point": 9.673819541931152 }, "id": "EFPKPENHBP_670", "locus": "GCA000186005_00642" }, { "type": "cds", "sequence": "contig_1", "start": 355744, "stop": 355998, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAG", "aa": "MVVESNNLLPALKVKHFISFKKLQKIYKKTLHIQLLENECYLYSLINTDHCFIKKTKVKNIQYFALLQDFIEKFKKVRAFLNLN", "aa_hexdigest": "4efa76a6fa17cc2ee86ba8a17d6f4548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10091.987599999991, "isoelectric_point": 9.732163429260254 }, "id": "EFPKPENHBP_671", "locus": "GCA000186005_00643" }, { "type": "cds", "sequence": "contig_1", "start": 356089, "stop": 356424, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0", "UniRef:UniRef90_C4XER0" ], "nt": "ATGCAAAGAAAAAAAATCAAAAATATTTTAATTGTTTCTTCTTTTGCTATTCCAATTTCAATGACTATTTCAGCAAGTGGCTGCAATTTAAAAGCTATTGAAAATAAATTCAATAATGAGATAGTGAAATCAAAAGAATGTGTCAAAGAATTTGAAAATAAAAAAGATATTCAAGAAGAAAAATATGAAGAATTAAGAGAAAGTTTAGTGAATTTTTCTAAATATATTGTTCAAGTAGATACTTTAAAACCTGGTCCAGTATCACTAAGAAATAGAAAAATATATCAATATGCAACAAGATTACTTCAAAGTTTTCAACAATTGCATAAAAATTGA", "aa": "MQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKN", "aa_hexdigest": "716207286e8245e04cf5dfd285df89da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 356002, "stop": 356430, "observations": { "mutation": [ 356025, 356091 ], "stop": [ 356025, 356091 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER0", "query_cov": 1.0, "subject_cov": 0.7816901408450704, "identity": 1.0, "score": 209.0, "evalue": 5.24e-68, "gene_start": 1, "gene_end": 111, "reference_start": 3, "reference_end": 113, "reference_sequence": "MEMQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKNWYEYILKNGNDENCDDKMTQELWDFYLKK" }, "paralog": false, "identity": 0.9788732394366197, "score": 276.0, "evalue": 7.8e-97, "description": "Internal stop codon at 356025, 356091. Nonsense mutation around 356025, 356091.", "psc": { "uniref90_id": "UniRef90_C4XER0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER0", "UniRef:UniRef50_C4XER0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0" ], "product": "Lipoprotein" } }, "id": "EFPKPENHBP_672", "locus": "GCA000186005_00644" }, { "type": "cds", "sequence": "contig_1", "start": 356733, "stop": 357296, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATATAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGA", "aa": "MKKANKLIISLGTAFAALSPVVLSVGCGGGGSSNLDSINGESTPSNENSAYANDKKGYNIKANDKVQIATTFSKGRAQYEALNDIIKNYNRLMKDQPGFKEVEQVSLGSGYDGQADVVRKKITAKEAQMPNLLLNYSSLASSLAESGVLLNFTTKENGGKESISNSDFAESFSRANRTTSKINNGGS", "aa_hexdigest": "d2fd6ad824e216f376b320711043b5f1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19829.867400000006, "isoelectric_point": 9.07793827056885 }, "id": "EFPKPENHBP_673", "locus": "GCA000186005_00645" }, { "type": "cds", "sequence": "contig_1", "start": 357582, "stop": 357704, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGA", "aa": "MFEKSSAVTTLEGPHALGIDDITGFIQTLGYASVGADPES", "aa_hexdigest": "7925ceb9795947445814e282e18d460b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4126.508000000001, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_674", "locus": "GCA000186005_00646" }, { "type": "cds", "sequence": "contig_1", "start": 357759, "stop": 357998, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGGTATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGA", "aa": "MEATSQANSKFKELYDMFVPKLKSQSVVFQQSGAYTSSEQIRHNFMFGIGSTAGYTHNFAKKSETSIEIKNSEGKFSSG", "aa_hexdigest": "1a9513a8c98dbbc81e859a3546bded5e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8782.685499999998, "isoelectric_point": 9.005153465270997 }, "id": "EFPKPENHBP_675", "locus": "GCA000186005_00647" }, { "type": "cds", "sequence": "contig_1", "start": 358221, "stop": 358556, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGA", "aa": "MIETKNKDDIKTLDAALADGKLVKVGEFVNNQNKEAILYLAFGKGAGISGLKIVEKGLDSLLEKDEFFKFATPSKTKDTDAKNVYYAQGPSLFGIRSSDNAMNQATRMFVK", "aa_hexdigest": "df6c7e4fc1bd61b7dac4879eb2adff91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12183.815599999984, "isoelectric_point": 8.776741981506348 }, "id": "EFPKPENHBP_676", "locus": "GCA000186005_00648" }, { "type": "cds", "sequence": "contig_1", "start": 358641, "stop": 358838, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGA", "aa": "MFSEQASYIFAVKDFEKTPMTTTNPYLRECFEMFKKTIEDPEHNAIYEEYGDPNGDAFRGALGTA", "aa_hexdigest": "ccd26344ed9bce850cc6cb66f87f58e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.2107, "isoelectric_point": 4.482516288757325 }, "id": "EFPKPENHBP_677", "locus": "GCA000186005_00649" }, { "type": "cds", "sequence": "contig_1", "start": 359017, "stop": 359427, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGA", "aa": "MKFKNNTLNFCLNTIVPSMAIVVCSSCQSKDDKIYNEAFKEDIKFDYVAYNPATGKYDIVDKNIENQLTTSLNNLSNISREFTYDGEEYKLEIDTISLARTFDKNKTFSENLKDPNFINNQREYTYKIFKKNPKGK", "aa_hexdigest": "5ab25517ebd08e93a55a015ffe71c73a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15859.692099999982, "isoelectric_point": 7.595303535461427 }, "id": "EFPKPENHBP_678", "locus": "GCA000186005_00650" }, { "type": "cds", "sequence": "contig_1", "start": 359458, "stop": 359559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGA", "aa": "MTKKFDVKLNLVDNFDKMDTRIDYSKLQATAYD", "aa_hexdigest": "f428e6a48ff83c30e7cda2c2c0749920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3928.4445000000005, "isoelectric_point": 6.022057151794433 }, "id": "EFPKPENHBP_679", "locus": "GCA000186005_00651" }, { "type": "cds", "sequence": "contig_1", "start": 359665, "stop": 359865, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "ATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGA", "aa": "MVVETIRIQGVDTPEKAVGKEIAPIFEKRFAEKSSEFANVNLPAGTKVRFIYSNKDAFKRIVAHLF", "aa_hexdigest": "33a7fc38db56d2043591462342e6f0ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 61, "aa_cov": 0.9242424242424242, "hmm_cov": 0.9180327868852459, "evalue": 2.0732746787270486e-06, "score": 24.523019790649414, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7453.5775, "isoelectric_point": 9.519997978210448 }, "id": "EFPKPENHBP_680", "locus": "GCA000186005_00652" }, { "type": "cds", "sequence": "contig_1", "start": 359890, "stop": 360195, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAG", "aa": "MYEYSAAITRFGFTLPYADGTELINMTNAGKTQHYTFLQIGEAFEAAMAEKTGFFKCLSDPDDISRYVYLSKRNGKYTIFQKKSNSNVFKFKDKAYKNPIN", "aa_hexdigest": "5661ce40177eeabb1f7757512e22f194", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11612.045599999985, "isoelectric_point": 9.162327384948732 }, "id": "EFPKPENHBP_681", "locus": "GCA000186005_00653" }, { "type": "cds", "sequence": "contig_1", "start": 360239, "stop": 360679, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGA", "aa": "MIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDAD", "aa_hexdigest": "90bd6f8ed3309e634846891e505fe558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.547945205479452, "hmm_cov": 0.9634146341463414, "evalue": 1.0891868473894444e-20, "score": 70.98065948486328, "start": 61, "stop": 140 } ], "seq_stats": { "molecular_weight": 16553.856099999983, "isoelectric_point": 9.329300498962404 }, "id": "EFPKPENHBP_682", "locus": "GCA000186005_00654" }, { "type": "cds", "sequence": "contig_1", "start": 360890, "stop": 361780, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3", "UniRef:UniRef90_C4XER3" ], "nt": "ATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAACAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGA", "aa": "MSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQ", "aa_hexdigest": "98f83c74bab74ce76359384bfbb2f653", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 360590, "stop": 362083, "observations": { "mutation": [ 360677, 360788, 361778 ], "stop": [ 360677, 360788, 361778 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER3", "query_cov": 1.0, "subject_cov": 0.42105263157894735, "identity": 1.0, "score": 522.0, "evalue": 4.43e-180, "gene_start": 1, "gene_end": 296, "reference_start": 223, "reference_end": 518, "reference_sequence": "MKGKNMIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDADWQNKVLDRKTEAIINIRNIYLKSLGASDLEIKQINEAWKIYKSIYRETQSELANYRVELREKFDKAFTDYKMSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQWIRDIQQKLGITTVFVTHDQEEAMSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK" }, "paralog": false, "identity": 0.993963782696177, "score": 940.0, "evalue": 0.0, "description": "Internal stop codon at 360677, 360788, 361778. Nonsense mutation around 360677, 360788, 361778.", "psc": { "uniref90_id": "UniRef90_C4XER3", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER3", "UniRef:UniRef50_C4XER3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_683", "locus": "GCA000186005_00655" }, { "type": "cds", "sequence": "contig_1", "start": 361847, "stop": 362335, "strand": "+", "frame": 2, "gene": null, "product": "TOBE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "nt": "ATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAA", "aa": "MSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK", "aa_hexdigest": "803def7f3858fe188acb441811a784e7", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C8186B", "query_cov": 0.808641975308642, "subject_cov": 0.9492753623188406, "identity": 0.534, "score": 130.0, "evalue": 7.14e-36, "valid": false, "product": "TOBE domain-containing protein", "uniref50_id": "UniRef50_UPI0024C8186B" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C8186B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "product": "TOBE domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_684", "locus": "GCA000186005_00656" }, { "type": "cds", "sequence": "contig_1", "start": 362475, "stop": 362717, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGA", "aa": "MPTLLVITAFTLVPFIINIIQSFTVQNAKNGVLHNFESLFKEPKFAVGVRNSILYAIVVLPFVMMVSLLISSCIANVYRK", "aa_hexdigest": "10afcda7012ffb74a434f2cbb33af95f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8944.721199999996, "isoelectric_point": 9.744928169250485 }, "id": "EFPKPENHBP_685", "locus": "GCA000186005_00657" }, { "type": "cds", "sequence": "contig_1", "start": 362964, "stop": 363344, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGA", "aa": "MLSVDKNLYKSASIDGIGPVKQFFTITLPSIKSTTTFLVTMGIINGIKVFPLALFNNRPEEAVLKGASTLMIFVYYYTSLGGAENLSLAAAGSILLFIIGIVYSTIIRGGFGTIMRVSLNLGESNV", "aa_hexdigest": "8b9d57ddc326d6fd3e561ff740d2ff53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13488.749299999985, "isoelectric_point": 9.346255683898928 }, "id": "EFPKPENHBP_686", "locus": "GCA000186005_00658" }, { "type": "cds", "sequence": "contig_1", "start": 363442, "stop": 363600, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGA", "aa": "MLLGLFLKLLALTFFALIILFPFFFMLMISLMPETQAKTLQSSFSIIPKSLT", "aa_hexdigest": "c9463c2ae26de4d5c9eac5ef58a0baf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5928.348499999999, "isoelectric_point": 9.700251579284668 }, "id": "EFPKPENHBP_687", "locus": "GCA000186005_00659" }, { "type": "cds", "sequence": "contig_1", "start": 363859, "stop": 364092, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGA", "aa": "MALKNNFIGAMVIIAVPFVASIFNALMFKNAFESIPSRIKEVALVDGAAGAKYLFKIAIPMVTPTTLTVVILTALAA", "aa_hexdigest": "b984adcf4cc5919a3b14d1bcd3edaec6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8140.814599999996, "isoelectric_point": 9.69799518585205 }, "id": "EFPKPENHBP_688", "locus": "GCA000186005_00660" }, { "type": "cds", "sequence": "contig_1", "start": 364213, "stop": 364320, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAG", "aa": "MAGAVLVIIPMFIFYFAFRKRIMNAISRQGSTIKG", "aa_hexdigest": "cc64338f5b148f1a69f97488c4a299f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3948.8095999999996, "isoelectric_point": 11.733906745910645 }, "id": "EFPKPENHBP_689", "locus": "GCA000186005_00661" }, { "type": "cds", "sequence": "contig_1", "start": 364619, "stop": 364795, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGA", "aa": "MNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPY", "aa_hexdigest": "c912bcb3fc7340d47c9f219846e6fca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6705.986799999999, "isoelectric_point": 9.76465549468994 }, "id": "EFPKPENHBP_690", "locus": "GCA000186005_00662" }, { "type": "cds", "sequence": "contig_1", "start": 364838, "stop": 365866, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7", "UniRef:UniRef90_C4XER7" ], "nt": "TTGGCTTACAACTTTTTTGCTCCACATCGTGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGA", "aa": "MAYNFFAPHRAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNK", "aa_hexdigest": "50a6cd77fe95201787ece5d183be7d81", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 364607, "stop": 366115, "observations": { "mutation": [ 364793, 365864, 365987 ], "stop": [ 364793, 365864, 365987 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER7", "query_cov": 1.0, "subject_cov": 0.6812749003984063, "identity": 0.9940000000000001, "score": 674.0, "evalue": 4.38e-242, "gene_start": 1, "gene_end": 342, "reference_start": 78, "reference_end": 419, "reference_sequence": "MRRIMNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPYWLQKNAKDKTSNQLELAYNFFAPHHAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNKWKIFSLSNFFDNLRKKFDRTNPVDEEFTKDKLITYDAFPSDWTFDLPNTVTKDDVDKMSIYLENGVKELKFTLTKIKQYSNIK" }, "paralog": false, "identity": 0.9880478087649402, "score": 998.0, "evalue": 0.0, "description": "Internal stop codon at 364793, 365864, 365987. Nonsense mutation around 364793, 365864, 365987.", "psc": { "uniref90_id": "UniRef90_C4XER7", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER7", "UniRef:UniRef50_C4XER7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7" ], "product": "Lipoprotein" } }, "id": "EFPKPENHBP_691", "locus": "GCA000186005_00663" }, { "type": "cds", "sequence": "contig_1", "start": 366196, "stop": 366888, "strand": "-", "frame": 1, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGAACCCAAATAGCGAAAGAGTTGACTTTGTTATTAAAATGCCTAATGGAGAAGCTAACTCTCAAAATGATCGTTACTTGCCTATTGATTGTAAGTTTCCACTTGATTCTTTTAATGCATATCAAGATGCAGACTCTAAAGAAGAAAGAATTAAAGCTCAAATTAACTTTAGAAAAGTTATTCTCGAACAAGCTAAAAGTATTAAAAGTAAATACATTATTAAAAATGTAACTACCAAGCAAGCAATTATGTATCTACCATCTGAAGCTATTTTTGCTTTAGCAGTTAGTGACAATGAATTGGTTTCAGCATTGAATAAAGATCATGATATTATGTTGGCTGGACCAAGTACAATTTTGATGATTATTCAAACCATTGCTTATGCAAATTATGGAATTAACATAAAACAAAATATTGACATTCTTTTAGATGCATTCTTATTTATTCGTCAAAGTGAAGATAATATTTATACCAAAATTGAACAAAGTATCAAGAAAAATGATAAAACAGGAGTTGAATTAAATAGAGCATTAAAAATAGTCGATAGAGTAAAGAAAAGAATTCAAAATTCTATTGATAGTGCACGAAAAAAAGGTATTATAAAAGAAGATTATGACATTGATAAAGTTGCAATGAATATGCCTATGATTGAAGATTTAAGTGATGAAAATGATGATAAAGAAGAAGAATAA", "aa": "MNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE", "aa_hexdigest": "fd352c1047aa627bcd1878a783a3777e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rmuC" ], "truncated": "3-prime", "pseudogene": { "start": 366196, "stop": 367188, "observations": { "mutation": [ 366963 ], "stop": [ 366963 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.5287356321839081, "identity": 1.0, "score": 431.0, "evalue": 2.12e-149, "gene_start": 1, "gene_end": 230, "reference_start": 206, "reference_end": 435, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.996969696969697, "score": 612.0, "evalue": 6.25e-223, "description": "Internal stop codon at 366963. Nonsense mutation around 366963.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "EFPKPENHBP_692", "locus": "GCA000186005_00664" }, { "type": "cds", "sequence": "contig_1", "start": 366961, "stop": 367503, "strand": "-", "frame": 1, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGGAAATAGGAATTCTAATTGTAGTAATATTGACTATGATTTTAGCTATTAGTAGTATTGTCGTTAGTTTCATCTTATATAAAAAAAATAAGACAGAAGTTATCGATCAAGACAAAATGACAAATAATATTAAAAATCAAATAACTGAAGATTTGTCTAGTCAATTTGTCAAATTCAATAAAAATGTTGATGAAAAAATTGCCAATTCATTCAACATGATTAATAAAAACTTTAGTGATTTAAGAGAAGAAATTGACAAAAAAATGAAATCGTTTGATGATTTAGTAATTAAGGAAGTGCAAATAATTAAAGGTGCTATTGATGAAAAATTTGAAAATGAAGTAGCTAAAAAGATGGAAGAAAACTTTAAAAATATTAAAGAAAACATGGAAAATCTTGACAAGAATTTAGTACGTTTTGAGACCTTACAAGATTCAGTTAATTCATTACAAAAAACTTTTGATGGAACAAAAAGTCGCGGTAATTATGGTGAGTTTAATTTAAAGAGTATTTTGAATGAACATTTATCAAAAGACTTGTGA", "aa": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDL", "aa_hexdigest": "a8011aee3ee43a45f6749d607917feee", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rmuC" ], "truncated": "5-prime", "pseudogene": { "start": 366658, "stop": 367503, "observations": { "mutation": [ 366963 ], "stop": [ 366963 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 300.0, "evalue": 1.42e-98, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.99644128113879, "score": 515.0, "evalue": 1.31e-185, "description": "Internal stop codon at 366963. Nonsense mutation around 366963.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "EFPKPENHBP_693", "locus": "GCA000186005_00665" }, { "type": "cds", "sequence": "contig_1", "start": 367683, "stop": 367856, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGA", "aa": "MKIVKLTKKEYKGKEFCVQYKTKGYYFIEHTKNGFSFVYKKFDQEKTFGYCDKFFQD", "aa_hexdigest": "e7e1c937be06c6942f6b226f649f2982", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7032.078200000002, "isoelectric_point": 9.369270896911619 }, "id": "EFPKPENHBP_694", "locus": "GCA000186005_00666" }, { "type": "cds", "sequence": "contig_1", "start": 368013, "stop": 368207, "strand": "+", "frame": 3, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "nt": "ATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAG", "aa": "MKKMIAKAKTTPARMIVLETETCNEKAIRFYQKFNFEVIGFDLYAYTNEDPKEHEVRVEMGLKI", "aa_hexdigest": "05d928382105f62a47e001beb02f031c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1H9U7D2", "query_cov": 0.953125, "subject_cov": 0.8356164383561644, "identity": 0.557, "score": 78.2, "evalue": 9.01e-18, "valid": false, "product": "Acetyltransferase (GNAT) family protein", "uniref50_id": "UniRef50_A0A358PH68" }, "pscc": { "uniref50_id": "UniRef50_A0A358PH68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_695", "locus": "GCA000186005_00667" }, { "type": "cds", "sequence": "contig_1", "start": 368251, "stop": 368535, "strand": "+", "frame": 1, "gene": null, "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0", "UniRef:UniRef90_C4XES0" ], "nt": "ATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGA", "aa": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDK", "aa_hexdigest": "3013309bd069b81796dec4c8369696a4", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 368251, "stop": 368790, "observations": { "mutation": [ 368533 ], "stop": [ 368533 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES0", "query_cov": 1.0, "subject_cov": 0.5251396648044693, "identity": 1.0, "score": 191.0, "evalue": 1.25e-60, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDKWVCAICDAPNVLYENHFIDDKVKYSSFPIIPLVGGKNRNEKMVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA" }, "paralog": false, "identity": 0.994413407821229, "score": 360.0, "evalue": 7.52e-130, "description": "Internal stop codon at 368533. Nonsense mutation around 368533.", "psc": { "uniref90_id": "UniRef90_C4XES0", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES0", "UniRef:UniRef50_A0A3P8MDU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0" ], "product": "Putative intracellular protease/amidase (Putative glutaminase)" } }, "id": "EFPKPENHBP_696", "locus": "GCA000186005_00668" }, { "type": "cds", "sequence": "contig_1", "start": 368656, "stop": 368790, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAA", "aa": "MVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA", "aa_hexdigest": "db2917e9cc46a20c64f75ee3c74e2c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4858.635200000001, "isoelectric_point": 6.068665122985839 }, "id": "EFPKPENHBP_697", "locus": "GCA000186005_00669" }, { "type": "cds", "sequence": "contig_1", "start": 369084, "stop": 369245, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTATACAAATCGAAACAACTTACTTTCATTCAAATACAATCAATTAGCTGAAAGAGTTGGCAAGATTAAAATTAGCAAAAAATCATACAAATATTCTTTTGATAAACTAGAAAATTTAATTAAATATTTCTCATTAAATTACAAAAGATGA", "aa": "MLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKR", "aa_hexdigest": "eb7899aac6b2d12912ba15f767e07369", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4464, "isoelectric_point": 10.054183006286618 }, "id": "EFPKPENHBP_698", "locus": "GCA000186005_00670" }, { "type": "cds", "sequence": "contig_1", "start": 369657, "stop": 370361, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1", "UniRef:UniRef90_C4XES1" ], "nt": "ATGCAAACAAATAATCAACAATTAAAAGAGCAAATTATCGTTTCAAACGATAATCTTTTAATTCGCAATGATACCTTTTTATTTAAAACAAATAACAAAGAAAAAATAGAACTCTCATCAATCTTGCGACTATTGCAAGCTATAAATAAAACTATTTTAAAAAGCAATAGTAACATTTTATTAAGCTTTGAAGGTCAAGAATTTTATAATGAAAATATTCAATATTTTAGCTCTTATTTTATCAAAAATAATCATAATGTTTTTAAGTATCACAATAACTTTTCAATTAGTTTGATTTTAGATGAATATGCCTATAAAAAAGCTTCATATGACTTTTTAATCAAATTTAAAAATATTAAAAATGATAACTATATTACAATTCAAATATTTGATAAAAACTTTGAAATTTTGACCTCTGAAAACTTACAAAAAATTTATGATTATTATCAATTAAACAAATGGCAAAATAAAGAAATTGTCATCAAAGATCCTAAACTAGTTAATGAATTTGAACTGATTGATGAATTAGCTTCAAAAGAACAAATTCTTAAAGCTTTCGTTAATGTTAAACAAAGATATAAAACATTAAGTTACTTTGCTAGTGACAATAAATTTAGTCAAAGTATTGGTACAGAACTATTAAATAATTATCAAAATAAATACATTACAAACAATAAAAATATTTCGCTTTTAAACCTAAAATGA", "aa": "MQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLK", "aa_hexdigest": "8537e5c8cd94727749f5b0d6e3142810", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 369354, "stop": 370376, "observations": { "mutation": [ 369542, 369632, 369659 ], "stop": [ 369542, 369632, 369659 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES1", "query_cov": 1.0, "subject_cov": 0.45525291828793774, "identity": 1.0, "score": 446.0, "evalue": 5.89e-154, "gene_start": 1, "gene_end": 234, "reference_start": 6, "reference_end": 239, "reference_sequence": "MIGNFMQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLKWLTFFAASSWYRENKIQNIFHFDINSNLNVCLRLNDKFKWLNSHELVLIYLDFFLEEIKRNGIDISNSYVVVPQNTTYQILELLKQYKVKYYFYDLKNINKQLKNESCLFAYIDNKFIANPRYSTQFNNYYFFICLIWMLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKRWFTAFNAYRVWNDHKYMLFKLINNKKHQMIFYWDDVKGKLVIEYQMCCNFENISNTVWWDKLKLQILLFVLIKKAQKHKIEKEE" }, "paralog": false, "identity": 0.9882352941176471, "score": 664.0, "evalue": 5.32e-242, "description": "Internal stop codon at 369542, 369632, 369659. Nonsense mutation around 369542, 369632, 369659.", "psc": { "uniref90_id": "UniRef90_C4XES1", "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES1", "UniRef:UniRef50_C4XES1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 27937.44870000004, "isoelectric_point": 9.023269081115721 }, "id": "EFPKPENHBP_699", "locus": "GCA000186005_00671" }, { "type": "cds", "sequence": "contig_1", "start": 370396, "stop": 373176, "strand": "-", "frame": 1, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "RefSeq:WP_013354563.1", "SO:0001217", "UniParc:UPI0001E330BB", "UniRef:UniRef100_C4XES2", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_A0A3P8MFB5" ], "nt": "ATGAGTTCAAAAGAACAAGATAAAGAAAAAGAATACAAAAGCAAACTTAATCAAGAAGAAGAAAAAGAAAATGCAATCGAAGAATTAGATGAAGATTACATTCCTGATGAAGAGCTTTTTGTTGCTTTTAAACCACAAAAAGAAGAAACTAAAGTTATTGAAGGGGAGGAAGAAGAAGTTCCTCAAAATAAAGACAACTATGTAGTTCAACCACAACTTTTAGATGCACCTAAAGATGGTATTCATCCAGTTGAAGTTCACAAAGAAATGAAAAACTCATTCTTAGAATATGCAATGAGTGTTATTGTTTCTCGTGCTTTACCAGATGCTCGTGATGGACTTAAACCAGTACATAGACGTATTCTTTTTGATATGAATGAATTAGGAATTACATTTGGATCGCAACATAGAAAAAGCGCTCGTATTGTCGGGGACGTTTTAGGTAAGTACCACCCACATGGCGACAGTTCAGTTTATGAAGCTATGGTTCGTATGGCGCAAGATTTTAGTATGCGTTATCCTTTAGTTGATGGTCACGGTAACTTTGGATCTATTGATGGTGATGAAGCTGCTGCGATGCGTTATACTGAAGCAAGAATGAGCAAATTAGCTGCTGAAATGCTTGAAGGTATTAAAAAAGATACAGTAGATTTTGTTGATAACTATGATGCTAGTGAAAAAGAACCTTCAGTATTACCATCAAGATTCCCTAACCTTTTAGTTTCAGGTGGTAGTGGTATTGCTGTTGGTATGGCAACAAGTATTCCACCTCACAACTTAGGTGAAACTATTGATGCAACTATTGCTTTAGCTAACAATCCAGAAATTGATATTTATGGCTTAATGGAATTTTTACCTGGTCCAGACTTTCCTACTGGAGCTATGATTTTAGGCAATGCAGGTATTAAAGATGCATACTTAACAGGCAAGGGATCCATTCCAATCAGATCAATAGCTAAAATAGAAGAATACTCAAATGGTAAAAGCAAAATTATTGTTACTGAAATTCCTTATGAAGTTAAGAAAACTGCAATTATTGAAAAAATAGCTGAATTAGTTAAAGATAAACAAATCGAAGGTATCGCCGACTTACGTGATGAAAGTAGCCGTGAAGGTATTAGAATTGTTATTGACGTTAAAAAGAATGTTAATCCTCATGTTTTATTAAACAAGCTTTATCGTCAAACAAACTTACAAGTAAACTACAATGTTAACTTTGTTGCTTTAGTTGATGGTGAACCTAAAGTTTTAAACTTAAAACAAGCTTTAGAAGTTTACTTAAGACACCAAGAAGAAGTAGTGACTAGAAGATTAAAATTTGATTTAATTAAAGCTCAAGACCGTCTTCACATTTTAGAAGGTCTTAAAATTGCAGTTGAAAATATTGATGAAGTTATTGCAATTATTAAGAAATCTAAAACAGATGGTGAAGCTCAAGCTAACTTAGCAAAAAGATTTAATTTAACTGAAAAACAAACAAAAGCTATTGTTGACATGCGTTTAGGTCGTCTTACAGGACTTGCTATTGAAAACATGCTTGAAGAAATGGCAGAGTTAAGAAAAGAAATTGAAAGAATTGAATTTATTTTGGCTAACCATAGTGAATTAATTAAATTAATTGTTAAAGAATTAACTGAAATTAAAGAAAAATACAATGACAAACGTCGCACACAAATTAACTATGAAATTCAAGGTACTATTACTGATGAAGACTTGATTCCTCAACGTGACATTGTTATTACAACCAGCGCCAAAGGCTTTGTAAAAAGAATGGACTTAGAAGAATACAAAACTCAACGTCGTGGTGGAGTTGGTATTGGTACTATGAAAACATATGACGATGATGATATTGCCTCAATATTAACAACTACAACTCACACCGACTTACTATTATTCTCAAATCATGCTCGTGTCTACCGTGTTAGAGCTCATGAAATTCCAGAATTATCTCGTCAAAGTAAAGGTGTAGCATTCATTAATATTATTCCTTCACTTCGTGTTAAAGAAGGTGAAAGAATTGTTTCAATGCTTCCAGTAGACACTTACGATGAAGATCACTACTTATTTACAGCAACTAAAAATGGTATTATCAAGAAAACTAGATTAACTGAATACGAAAGAATTAACGCTAATGGTAAATATGCTTTCAACTTACGTGAAGGCGATGAATTAATTAGAGCAATGATTATTACTGATGAAGACTTAATTCTTTTAGCAAATGATGAAGAAAGAATTGTTAAATTCCAAGCTATTGACTTTAGACCAATTAGTAGAACTGCAACTGGTGTTAAAGGTATAACACTTGATAGCCATCAAACAGTTTCAAGCACTTCAACAAGTCAAGAAGGTGAATTAATCTTAACTATTGGTAAAAAAGGTTTTGGAAAACTTACTCACCACTCACTATTTAGATTAATTAAACGTGGTGGTAAAGGTGTTAAAGGAATTAATAGCAAATTAGCAGGCGACATTGTTTTTGCTAGATTCGTAAATCCTCAAGATGAACTTTTAATTATTACAACAAGTGGTTTAACAATTAGAACAGCTATTAGCCAAATTAGCCAAACTGGTCGGGCAGCTAAAGGTGTTAAAATAATTAACCTTAAAAAGCGTGAAGAAATTCAAGCTGTTGAAATCATTAAAATTGATGAAAAAACAGATCCAGAAGCAACTATGGCTGCAACTTCTGTTGTTCAAAGCATTTATGAACAAAGTCAAGAAATCAACTTAGATGATAACTCAAACGATGAAATTGATATTGATTCAAACAATGAAGAATAA", "aa": "MSSKEQDKEKEYKSKLNQEEEKENAIEELDEDYIPDEELFVAFKPQKEETKVIEGEEEEVPQNKDNYVVQPQLLDAPKDGIHPVEVHKEMKNSFLEYAMSVIVSRALPDARDGLKPVHRRILFDMNELGITFGSQHRKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSMRYPLVDGHGNFGSIDGDEAAAMRYTEARMSKLAAEMLEGIKKDTVDFVDNYDASEKEPSVLPSRFPNLLVSGGSGIAVGMATSIPPHNLGETIDATIALANNPEIDIYGLMEFLPGPDFPTGAMILGNAGIKDAYLTGKGSIPIRSIAKIEEYSNGKSKIIVTEIPYEVKKTAIIEKIAELVKDKQIEGIADLRDESSREGIRIVIDVKKNVNPHVLLNKLYRQTNLQVNYNVNFVALVDGEPKVLNLKQALEVYLRHQEEVVTRRLKFDLIKAQDRLHILEGLKIAVENIDEVIAIIKKSKTDGEAQANLAKRFNLTEKQTKAIVDMRLGRLTGLAIENMLEEMAELRKEIERIEFILANHSELIKLIVKELTEIKEKYNDKRRTQINYEIQGTITDEDLIPQRDIVITTSAKGFVKRMDLEEYKTQRRGGVGIGTMKTYDDDDIASILTTTTHTDLLLFSNHARVYRVRAHEIPELSRQSKGVAFINIIPSLRVKEGERIVSMLPVDTYDEDHYLFTATKNGIIKKTRLTEYERINANGKYAFNLREGDELIRAMIITDEDLILLANDEERIVKFQAIDFRPISRTATGVKGITLDSHQTVSSTSTSQEGELILTIGKKGFGKLTHHSLFRLIKRGGKGVKGINSKLAGDIVFARFVNPQDELLIITTSGLTIRTAISQISQTGRAAKGVKIINLKKREEIQAVEIIKIDEKTDPEATMAATSVVQSIYEQSQEINLDDNSNDEIDIDSNNEE", "aa_hexdigest": "c1907b31a1ae232dcb9284fadec2ec78", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E330BB", "ncbi_nrp_id": "WP_013354563.1", "uniref100_id": "UniRef100_C4XES2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330BB", "RefSeq:WP_013354563.1", "UniRef:UniRef100_C4XES2" ] }, "ips": { "uniref100_id": "UniRef100_C4XES2", "uniref90_id": "UniRef90_A0A3P8MFB5" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MFB5", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "EFPKPENHBP_700", "locus": "GCA000186005_00672" }, { "type": "cds", "sequence": "contig_1", "start": 373594, "stop": 373698, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGA", "aa": "MTINRMITKFDEENPGILVKMQKAHIAPSFLAYI", "aa_hexdigest": "52ceff1930d05be9e6b2bd35a8ec370e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3921.6504999999997, "isoelectric_point": 8.211289787292483 }, "id": "EFPKPENHBP_701", "locus": "GCA000186005_00673" }, { "type": "cds", "sequence": "contig_1", "start": 373839, "stop": 373997, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACATAACTTTTTTATCAAGTGTAATATTTTTACGATTTAAAATCAATATGACTATTCCAATTAATGGATTAATTGCATGTACAATTGTGCTTAAAGCTAATCTAAAGGAACCATACTCTGATCCAAATATTTTTGGAAAAATTAAAGTTCAATAA", "aa": "MYITFLSSVIFLRFKINMTIPINGLIACTIVLKANLKEPYSDPNIFGKIKVQ", "aa_hexdigest": "99409788cfdf9cb41ca922d09144a65b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.127000000001, "isoelectric_point": 9.655059242248537 }, "id": "EFPKPENHBP_702", "locus": "GCA000186005_00674" }, { "type": "cds", "sequence": "contig_1", "start": 374110, "stop": 374256, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGTGATATTGTAGTTTATGGCTTTTTAAACTTTATTGAACCATTTTTTTATCGCGGAGGTAATATTGCAATAGTTATTACATTAGACTTATTAATGATTATAATAGCAGTCTTTGTACCTATTGGCTTAAGTTATTTTTGA", "aa": "MDSDIVVYGFLNFIEPFFYRGGNIAIVITLDLLMIIIAVFVPIGLSYF", "aa_hexdigest": "2925e74b6286f27787552784d1df5c43", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5431.448099999998, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_703", "locus": "GCA000186005_00675" }, { "type": "cds", "sequence": "contig_1", "start": 374545, "stop": 375036, "strand": "+", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "nt": "ATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGA", "aa": "MRYRGPVFKKSRRYGFSILETGKEFSKGRKRTYAPGQHGDKRVKLSDYGLHLYEKQKVKYLYGLNEKQMHKYYIRATKIKGVTGTVLLQLLESRLDNLVYRAGFASTRRQARQLVSHGHFTLNGHKADIPSMNVEVGSKIQLKEKSQKNKIIEANLKDRSAAK", "aa_hexdigest": "fee83a092c3b0cd7e14f7ad20f689366", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A974WKF8", "query_cov": 1.0, "subject_cov": 0.8232323232323232, "identity": 0.816, "score": 258.0, "evalue": 8.85e-86, "valid": false, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [], "id": "EFPKPENHBP_704", "locus": "GCA000186005_00676" }, { "type": "cds", "sequence": "contig_1", "start": 375161, "stop": 375400, "strand": "+", "frame": 2, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354566.1", "SO:0001217", "UniParc:UPI0001A8C219", "UniRef:UniRef100_C4XES6", "UniRef:UniRef50_A0A449BAA4", "UniRef:UniRef90_A0A3P8KXC8" ], "nt": "ATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAA", "aa": "MKKNIHPQYFTVEATCSTCGKKFQFGSTKKSISVDVCSGCHVVYTGDRTKTKATGMIDKFNQRLAKQKAAIEKSKGKKK", "aa_hexdigest": "a74ba465a9352977cd327abffc417088", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C219", "ncbi_nrp_id": "WP_013354566.1", "uniref100_id": "UniRef100_C4XES6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C219", "RefSeq:WP_013354566.1", "UniRef:UniRef100_C4XES6" ] }, "ips": { "uniref100_id": "UniRef100_C4XES6", "uniref90_id": "UniRef90_A0A3P8KXC8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KXC8", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_A0A449BAA4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAA4" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "EFPKPENHBP_705", "locus": "GCA000186005_00677" }, { "type": "cds", "sequence": "contig_1", "start": 375587, "stop": 376381, "strand": "+", "frame": 2, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "GO:0003677", "GO:0006355", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_N9VB78", "UniRef:UniRef90_C4XES7" ], "nt": "ATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGA", "aa": "MANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSI", "aa_hexdigest": "78cd355c64ac915be62402a5bae62818", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "3-prime", "pseudogene": { "start": 375584, "stop": 376609, "observations": { "mutation": [ 376379 ], "stop": [ 376379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES7", "query_cov": 1.0, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 488.0, "evalue": 9.56e-173, "gene_start": 1, "gene_end": 264, "reference_start": 2, "reference_end": 265, "reference_sequence": "MMANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSIWQTIESEIDEDENIKIAICDDHSTFISKKLETQSKIKEIALVGTSRMDYEKGFSALKALEEFIENKPCKSKDEGDK" }, "paralog": false, "identity": 0.9941348973607038, "score": 647.0, "evalue": 1.03e-237, "description": "Internal stop codon at 376379. Nonsense mutation around 376379.", "psc": { "uniref90_id": "UniRef90_C4XES7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_N9VB78", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES7", "UniRef:UniRef50_N9VB78", "KEGG:K03705", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_N9VB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VB78" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "EFPKPENHBP_706", "locus": "GCA000186005_00678" }, { "type": "cds", "sequence": "contig_1", "start": 376517, "stop": 376609, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAA", "aa": "MDYEKGFSALKALEEFIENKPCKSKDEGDK", "aa_hexdigest": "c21a28a2550c58b0a55b5ed381902f00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3450.8446000000013, "isoelectric_point": 4.812637138366699 }, "id": "EFPKPENHBP_707", "locus": "GCA000186005_00679" }, { "type": "cds", "sequence": "contig_1", "start": 376609, "stop": 377862, "strand": "+", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "KEGG:K03687", "RefSeq:WP_013354568.1", "SO:0001217", "UniParc:UPI0001E330BF", "UniRef:UniRef100_A0AB32XBD6", "UniRef:UniRef50_C4XES8", "UniRef:UniRef90_C4XES8" ], "nt": "ATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACGGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAA", "aa": "MKVVPNLNKFKTGEGIIANISVYNSSNAYQKQLSKEKVSFVLGENTFLPNFDNNIVEQTVRPVLDFVVTFPKSYEVAEFKDKTFRFVIEILDYQDSEYVKAYKQMLALREEIQKLNVASGNHLKELEELKAKNEKQMSEISNLKTENDKHTKDSADLKNQYQEQEKTIQKLIEDNKKFIADNNALKEDNENKGKQIKELMSGKTTKLMEQTSEHEAKGVNPAVLRKQVDVLRKQLEEQSIELKELRKNKAPEAVQRVTVPKEEVDKIKQYALQKFFEDFSTHYTTLKGAVKAGAKSENSSVKNYVVGFEMILNLINGVLEKHGIKAIEPKIGDEFDPNTQKVLEVEECKDKKHNTIVKVSAIGFKLHDRVIKPALVVIAQDSSAKDKKLAAETKKPEAPKAKETTKKTETKKTTTKK", "aa_hexdigest": "a78c5899fed54e642ee4f195fc36391c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330BF", "ncbi_nrp_id": "WP_013354568.1", "uniref100_id": "UniRef100_A0AB32XBD6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330BF", "RefSeq:WP_013354568.1", "UniRef:UniRef100_A0AB32XBD6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBD6", "uniref90_id": "UniRef90_C4XES8" }, "psc": { "uniref90_id": "UniRef90_C4XES8", "product": "Protein GrpE", "uniref50_id": "UniRef50_C4XES8", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_C4XES8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES8" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "EFPKPENHBP_708", "locus": "GCA000186005_00680" }, { "type": "cds", "sequence": "contig_1", "start": 378082, "stop": 378450, "strand": "+", "frame": 1, "gene": null, "product": "Large conductance mechanosensitive channel", "db_xrefs": [ "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_A0A077LBI7", "UniRef:UniRef90_C4XES9" ], "nt": "ATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAA", "aa": "MFMLAIGLLLGTAFNAVVSSLANDVIMAAIAKAFNVDEVKDLKAGPILIGKFLAALISFLIVATVIFILLVVVFLVKNAIETRKFKKNPPVAEEPKPTTEELILAEIKKLNERLEKLEQPKK", "aa_hexdigest": "4071d75cdfd5135218d201d4f67d5683", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XES9", "query_cov": 1.0, "subject_cov": 0.8776978417266187, "identity": 1.0, "score": 209.0, "evalue": 1.06e-67, "valid": true, "product": "Large conductance mechanosensitive channel", "uniref50_id": "UniRef50_A0A077LBI7", "kegg_orthology_id": "K03282" }, "pscc": { "uniref50_id": "UniRef50_A0A077LBI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBI7" ], "product": "Large-conductance mechanosensitive channel" }, "genes": [], "id": "EFPKPENHBP_709", "locus": "GCA000186005_00681" }, { "type": "cds", "sequence": "contig_1", "start": 378563, "stop": 378670, "strand": "+", "frame": 2, "gene": null, "product": "HTH-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1", "UniRef:UniRef90_A0AB32XCG9" ], "nt": "ATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAA", "aa": "MVIFDLFNYNKGLYGYRRITFVLRNKEVMINYKKD", "aa_hexdigest": "09eee22433129b8a8b122a6da5976d4e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 378566, "stop": 378714, "observations": { "deletion": [ 378668 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCG9", "query_cov": 0.9428571428571428, "subject_cov": 0.6111111111111112, "identity": 0.848, "score": 63.9, "evalue": 7.18e-13, "gene_start": 2, "gene_end": 34, "reference_start": 4, "reference_end": 36, "reference_sequence": "MKKIIFDLFNYNKGLYGYRRITFALRNKGIMINHKKVQKLIKSLNLFGKTLRKK" }, "paralog": false, "identity": 0.8235294117647058, "score": 80.1, "evalue": 4.04e-22, "description": "Frameshift due to deletion around 378668.", "psc": { "uniref90_id": "UniRef90_A0AB32XCG9", "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCG9", "UniRef:UniRef50_C4XEC1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1" ], "product": "HTH-like domain-containing protein" } }, "id": "EFPKPENHBP_710", "locus": "GCA000186005_00682" }, { "type": "cds", "sequence": "contig_1", "start": 378902, "stop": 379042, "strand": "+", "frame": 2, "gene": null, "product": "Integrase catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0", "UniRef:UniRef90_C4XET0" ], "nt": "ATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGA", "aa": "MNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQG", "aa_hexdigest": "8f2563d043fc78156bf87b6c69c55c2d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 378749, "stop": 379096, "observations": { "mutation": [ 378839, 379040 ], "stop": [ 378839, 379040 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET0", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 94.0, "evalue": 7.5e-24, "gene_start": 1, "gene_end": 46, "reference_start": 52, "reference_end": 97, "reference_sequence": "MPNLLLDKENITEDFFRYKRNFSNEKYLDIWGTDVTEFKLINEEKIYFSPVMNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQGWQYTHQDYIDYLKEKNAI" }, "paralog": false, "identity": 0.9739130434782609, "score": 228.0, "evalue": 4.31e-79, "description": "Internal stop codon at 378839, 379040. Nonsense mutation around 378839, 379040.", "psc": { "uniref90_id": "UniRef90_C4XET0", "product": "Integrase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET0", "UniRef:UniRef50_C4XET0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0" ], "product": "Integrase catalytic domain-containing protein" } }, "id": "EFPKPENHBP_711", "locus": "GCA000186005_00683" }, { "type": "cds", "sequence": "contig_1", "start": 379442, "stop": 379639, "strand": "+", "frame": 2, "gene": null, "product": "Class IIb bacteriocin, lactobin A/cerein 7B family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6", "UniRef:UniRef90_C4XET1" ], "nt": "ATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGA", "aa": "MKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIK", "aa_hexdigest": "2b2fb9a0f6957ba4c47116ceac72589a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 379403, "stop": 379681, "observations": { "mutation": [ 379637 ], "stop": [ 379637 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET1", "query_cov": 1.0, "subject_cov": 0.6989247311827957, "identity": 1.0, "score": 127.0, "evalue": 4.98e-37, "gene_start": 1, "gene_end": 65, "reference_start": 14, "reference_end": 78, "reference_sequence": "MLPKRKVKNERRTMKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIKWDNSPATNGNIFYAW" }, "paralog": false, "identity": 0.9782608695652174, "score": 178.0, "evalue": 1.07e-59, "description": "Internal stop codon at 379637. Nonsense mutation around 379637.", "psc": { "uniref90_id": "UniRef90_C4XET1", "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET1", "UniRef:UniRef50_A0A3P8MFB6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6" ], "product": "Class IIb bacteriocin, lactobin A/cerein 7B family" } }, "id": "EFPKPENHBP_712", "locus": "GCA000186005_00684" }, { "type": "cds", "sequence": "contig_1", "start": 379961, "stop": 380581, "strand": "+", "frame": 2, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2", "UniRef:UniRef50_Q05426", "UniRef:UniRef90_Q05426" ], "nt": "ATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAA", "aa": "MKKPTAEHMINENIPFQKVFVVGANGEKVGVLSTREAIEMAKEQRLDLVLISVQPKPIARILDYGKFKYERKKKQKAAKEKQTVIQNRQIRLTPLIGEHDLNVKARKAKEFLLDGDRIKVSLKFRGRESARPELGHKTLEKFYKAVEDLADIAKEAELVNDRFLDMYLQPNKIKVNKYRKEHNLVDKNSDSQDKCASEEDTNEGEQ", "aa_hexdigest": "a0f29309140a45a2b7ddf14c8c28bc66", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330C1", "uniref100_id": "UniRef100_C4XET2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2" ] }, "ips": { "uniref100_id": "UniRef100_C4XET2", "uniref90_id": "UniRef90_Q05426" }, "psc": { "uniref90_id": "UniRef90_Q05426", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_Q05426", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q05426", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q05426" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "EFPKPENHBP_713", "locus": "GCA000186005_00685" }, { "type": "cds", "sequence": "contig_1", "start": 380583, "stop": 380771, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_013526795.1", "SO:0001217", "UniParc:UPI000016F938", "UniRef:UniRef100_Q05428", "UniRef:UniRef50_Q05428", "UniRef:UniRef90_Q05428" ], "nt": "ATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAA", "aa": "MPKMKTKSALKKRIKITGTGKIMREQAYRSHLSQNKTTKQKRQARKSVQMHSSDVKRFKALI", "aa_hexdigest": "cdc21f893c75ed1d684dbb06b25c3927", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000016F938", "ncbi_nrp_id": "WP_013526795.1", "uniref100_id": "UniRef100_Q05428", "db_xrefs": [ "SO:0001217", "UniParc:UPI000016F938", "RefSeq:WP_013526795.1", "UniRef:UniRef100_Q05428" ] }, "ips": { "uniref100_id": "UniRef100_Q05428", "uniref90_id": "UniRef90_Q05428" }, "psc": { "uniref90_id": "UniRef90_Q05428", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_Q05428", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmI" ], "id": "EFPKPENHBP_714", "locus": "GCA000186005_00686" }, { "type": "cds", "sequence": "contig_1", "start": 381156, "stop": 381461, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A622", "UniRef:UniRef100_A0AB32XBD7", "UniRef:UniRef50_C4XET5", "UniRef:UniRef90_C4XET5" ], "nt": "ATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCCCAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAA", "aa": "MQNNNIKILFKMNPKFRTFGKGSFLYNLNLENKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALLQLKDSLTKKNSK", "aa_hexdigest": "d5df08b61ab78c2622bc9f902d821f96", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A622", "uniref100_id": "UniRef100_A0AB32XBD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A622", "UniRef:UniRef100_A0AB32XBD7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBD7", "uniref90_id": "UniRef90_C4XET5" }, "psc": { "uniref90_id": "UniRef90_C4XET5", "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_715", "locus": "GCA000186005_00687" }, { "type": "cds", "sequence": "contig_1", "start": 381527, "stop": 382216, "strand": "+", "frame": 2, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFF0", "UniRef:UniRef90_C4XET6" ], "nt": "TTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGA", "aa": "MKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARL", "aa_hexdigest": "6c839917bf40975022c366e9fab0935c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 381458, "stop": 382459, "observations": { "mutation": [ 382214, 382394 ], "stop": [ 382214, 382394 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET6", "query_cov": 1.0, "subject_cov": 0.6815476190476191, "identity": 0.996, "score": 446.0, "evalue": 7.69e-157, "gene_start": 1, "gene_end": 229, "reference_start": 24, "reference_end": 252, "reference_sequence": "MTIELKQEFKKLFSIKSILEKIKLKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARLWQERKLETYYPIIYIDAVHFHIVDNNVVTKKATYVIMGINGDGQKEILGLYIRENESAKFWMSVLNALKNRGISKIDIICSQII" }, "paralog": false, "identity": 0.978978978978979, "score": 644.0, "evalue": 2.56e-237, "description": "Internal stop codon at 382214, 382394. Nonsense mutation around 382214, 382394.", "psc": { "uniref90_id": "UniRef90_C4XET6", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFF0", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET6", "UniRef:UniRef50_C4XFF0", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF0" ], "product": "Mutator family transposase" } }, "id": "EFPKPENHBP_716", "locus": "GCA000186005_00688" }, { "type": "cds", "sequence": "contig_1", "start": 382397, "stop": 382663, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "b1d2697471a467a119612aa745a6f757", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 9.227899805118591e-09, "score": 32.01397705078125, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10129.612099999993, "isoelectric_point": 8.806848716735843 }, "id": "EFPKPENHBP_717", "locus": "GCA000186005_00689" }, { "type": "cds", "sequence": "contig_1", "start": 383375, "stop": 383470, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGATCATGCTTCAACTACTTTCTTATTAAAACATAATGGTGAACCTATTTCATGA", "aa": "MPFSNTLFSYLRTVDHASTTFLLKHNGEPIS", "aa_hexdigest": "926f94348d4dc2b9400c4f72fc0640c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3524.9525000000003, "isoelectric_point": 6.694576072692871 }, "id": "EFPKPENHBP_718", "locus": "GCA000186005_00690" }, { "type": "cds", "sequence": "contig_1", "start": 383687, "stop": 383851, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAACATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAAGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCCTTCGATTTCATTAAATACAATAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKTSDSKIMFKNDFQSNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "dbccbc6003fce992d206baffb5cabe3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6355.1217000000015, "isoelectric_point": 8.963700294494632 }, "id": "EFPKPENHBP_719", "locus": "GCA000186005_00691" }, { "type": "cds", "sequence": "contig_1", "start": 384332, "stop": 384550, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTGCAAAAATTAATTCAATTAAAAATTATTTAGAAAATGATTATTTATTAGCCGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFAKINSIKNYLENDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "be262ada710dde32b74c0c884593f0b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8388.426699999996, "isoelectric_point": 4.867032051086425 }, "id": "EFPKPENHBP_720", "locus": "GCA000186005_00692" }, { "type": "cds", "sequence": "contig_1", "start": 384662, "stop": 384829, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAATTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGTTATTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEIYKNVNSTENFSVIQADKDNKVNFVKK", "aa_hexdigest": "cde530d978da5e11e1277961cd9f5198", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.042100000002, "isoelectric_point": 7.942263221740723 }, "id": "EFPKPENHBP_721", "locus": "GCA000186005_00693" }, { "type": "cds", "sequence": "contig_1", "start": 385652, "stop": 386191, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGTGGTGTTTTAGTAAATAGAAGTGTTATTATAAAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACAAAGGAATATATAAATCGTTATGTTAACACTTTTGTCAAAACAATTGAAAGTACAAAATTATTTAACAAATCTCAAATTTTAGACTTAAGCAGCAAATCATTAACGCCTATTGTTAATGCTCTTAATGAATTTAACTCATTTATTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAATTTATTCTAAACGCTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATATTTCAAATGTTGATTCAATTTAA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIKDIIKACKTLEQSYGNTKEYINRYVNTFVKTIESTKLFNKSQILDLSSKSLTPIVNALNEFNSFIESSNQISFEKLDEFINKIQFILNAENDLTTSIQSVIKEFLNAYISNVDSI", "aa_hexdigest": "4059ffbaf415295375c49c6a9c4db444", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 385361, "stop": 386191, "observations": { "mutation": [ 385561, 385654 ], "stop": [ 385561, 385654 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.6485507246376812, "identity": 0.927, "score": 316.0, "evalue": 3.51e-107, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.8840579710144928, "score": 466.0, "evalue": 8.7e-169, "description": "Internal stop codon at 385561, 385654. Nonsense mutation around 385561, 385654.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20163.653100000007, "isoelectric_point": 4.97110424041748 }, "id": "EFPKPENHBP_722", "locus": "GCA000186005_00694" }, { "type": "cds", "sequence": "contig_1", "start": 386246, "stop": 386710, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATCTTGGTCACCACTTTGGTAACAAAAGTTATATGAGAACTGTTAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHLGHHFGNKSYMRTVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHV", "aa_hexdigest": "17efb620cb5c875ee72ec80fcc4d0074", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 386210, "stop": 386971, "observations": { "mutation": [ 386248, 386725, 386740, 386764, 386842 ], "stop": [ 386248, 386725, 386740, 386764, 386842 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.968, "score": 280.0, "evalue": 1.78e-93, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9209486166007905, "score": 441.0, "evalue": 1.74e-159, "description": "Internal stop codon at 386248, 386725, 386740, 386764, 386842. Nonsense mutation around 386248, 386725, 386740, 386764, 386842.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17770.864899999997, "isoelectric_point": 6.302102851867676 }, "id": "EFPKPENHBP_723", "locus": "GCA000186005_00695" }, { "type": "cds", "sequence": "contig_1", "start": 386840, "stop": 386971, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGGATTGTTGGTGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAGTTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "EFPKPENHBP_724", "locus": "GCA000186005_00696" }, { "type": "cds", "sequence": "contig_1", "start": 387338, "stop": 387553, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGGCAAAAGACAAGCTATACCTGAAAAGGAATTAATTAAACTTGTTGAAGAATTAAAAAGCTACAAAAACACCAAATTTAGTATCATTTTTCGTATTTTTCTTTCAACTGGTATTAGAGTTGGAGAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAGAGAATTGA", "aa": "MDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKEN", "aa_hexdigest": "c32fee22b7da91596b9956dae8297d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8507.892799999994, "isoelectric_point": 9.155622673034667 }, "id": "EFPKPENHBP_725", "locus": "GCA000186005_00697" }, { "type": "cds", "sequence": "contig_1", "start": 387620, "stop": 388075, "strand": "+", "frame": 2, "gene": null, "product": "Tyr recombinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG84", "UniRef:UniRef90_C4XG84" ], "nt": "ATGCCTTTAATAGGTAATCTTAGAGAAGATATATTAGAAAATATAGATAAATTACGTATTTCTGAAAAATCAATTAAAAATATGTTTTATCTTTTTAAAAAACATATTAGTGAAAAATATCCTGATTTTAAATATAAAATCTCGGCTCACGTCTTAAGACATAATTATGCAACTAAAGCTTTTTATAATTTAAAAAATAATGATTTAGTAGCCAAATTATTAGGACACACAAATTCAAATATTACTCAAAAAACTTATATTGATTATGATAAAAGTATCATGGATGAGTATGCTATTTTAGCCATACAAGATCAAAAAATAGGCTTAAATTCTATGCAATTATTAAATATAAATAATAATTTAGAGCTTACAATTATCAAATTAGAGAAAGAAAATTGCAGCTTAAAAGAAGAAATTGAGATGCTAAAAAATAAAATAAAAAAACTAAAAAAATAA", "aa": "MPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKKLKK", "aa_hexdigest": "fa9748b6dc4e581eea21cfd8ec3835b9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 387320, "stop": 388075, "observations": { "mutation": [ 387551 ], "stop": [ 387551 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG84", "query_cov": 1.0, "subject_cov": 0.4326647564469914, "identity": 1.0, "score": 288.0, "evalue": 2.17e-95, "gene_start": 1, "gene_end": 151, "reference_start": 199, "reference_end": 349, "reference_sequence": "MCLYIKIFIRHFWRSKNMKKNNNFLDIFLNNIYSMVQKNQRSYQTFKQYKRYLSSLKNWNNAKQLIVRMNRELANNKISNNTKLTKRNIFSSFLNWFCKITNTNMDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKENWSFIIKTLKNNNPRYFRIPSKLEMPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKKLKK" }, "paralog": false, "identity": 0.9960159362549801, "score": 490.0, "evalue": 1.54e-177, "description": "Internal stop codon at 387551. Nonsense mutation around 387551.", "psc": { "uniref90_id": "UniRef90_C4XG84", "product": "Tyr recombinase domain-containing protein", "uniref50_id": "UniRef50_C4XG84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG84", "UniRef:UniRef50_C4XG84" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG84" ], "product": "Tyr recombinase domain-containing protein" } }, "id": "EFPKPENHBP_726", "locus": "GCA000186005_00698" }, { "type": "cds", "sequence": "contig_1", "start": 388374, "stop": 388505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGAATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "EFPKPENHBP_727", "locus": "GCA000186005_00699" }, { "type": "cds", "sequence": "contig_1", "start": 388635, "stop": 389099, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATATTGGTCACCACTTTGGTAACAAAAGTTATATAAGAGCTGTTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAGGAATATCAAAACACTTTAAGTAAAATTGAAGAAGAAGCACTTTCAAGCAACATAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCCGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHIGHHFGNKSYIRAVKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKEYQNTLSKIEEEALSSNIKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "667b65428f8842532550fc40bf57fbba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 388374, "stop": 389135, "observations": { "mutation": [ 388503, 388581, 388605, 388620, 389097 ], "stop": [ 388503, 388581, 388605, 388620, 389097 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.9420000000000001, "score": 274.0, "evalue": 2.4e-91, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9090909090909091, "score": 437.0, "evalue": 8.19e-158, "description": "Internal stop codon at 388503, 388581, 388605, 388620, 389097. Nonsense mutation around 388503, 388581, 388605, 388620, 389097.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17763.89219999999, "isoelectric_point": 6.07150707244873 }, "id": "EFPKPENHBP_728", "locus": "GCA000186005_00700" }, { "type": "cds", "sequence": "contig_1", "start": 389154, "stop": 389786, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGCGGTGTTTTAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTTAAAACAATTGAAAATACAAAATTATTTAATAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTCGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCAAAACAAATTATTGATCAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNVDSIETTVKLAKLNNSKQIIDQFVQKSKIELSSLK", "aa_hexdigest": "fb7e6d4e1dd83e29bd5a97f7ba3a45f7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 389154, "stop": 389984, "observations": { "mutation": [ 389784 ], "stop": [ 389784 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.976, "score": 381.0, "evalue": 2.31e-132, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9637681159420289, "score": 508.0, "evalue": 1.48e-184, "description": "Internal stop codon at 389784. Nonsense mutation around 389784.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23671.59690000002, "isoelectric_point": 5.213408851623535 }, "id": "EFPKPENHBP_729", "locus": "GCA000186005_00701" }, { "type": "cds", "sequence": "contig_1", "start": 390516, "stop": 390683, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTATTGTTCAAGCTGATAAAGACAATAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEAYKNVNSTENFSIVQADKDNKVNFVKK", "aa_hexdigest": "7b41b65147eabf9a36bdb16c44d99af6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6214.962400000002, "isoelectric_point": 7.942263221740723 }, "id": "EFPKPENHBP_730", "locus": "GCA000186005_00702" }, { "type": "cds", "sequence": "contig_1", "start": 390795, "stop": 391016, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAATTAAAAATAATCAAATTTCTAAATTAGATAGTCAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAACATTGATTTAATTAAAAATTATTTAAATGAAACAGATTATTTATTAGCTGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MIAIKNNQISKLDSQGLKALQQLDGGVDFTNIDLIKNYLNETDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "16de9c6bd8d0e41364b20c1cc8b94209", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8529.637399999998, "isoelectric_point": 4.791663551330567 }, "id": "EFPKPENHBP_731", "locus": "GCA000186005_00703" }, { "type": "cds", "sequence": "contig_1", "start": 391461, "stop": 391667, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAATAACTGAAAAAATGCTTTTAAATATCAAAAAAATATCAGATTCTAAAATAATGCTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGAAAGATGA", "aa": "MAKLIEYEKTITEKMLLNIKKISDSKIMLKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEER", "aa_hexdigest": "f64bc6f4bc27e9b20bf6785b8ea22649", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8181.370100000003, "isoelectric_point": 8.802851676940918 }, "id": "EFPKPENHBP_732", "locus": "GCA000186005_00704" }, { "type": "cds", "sequence": "contig_1", "start": 392488, "stop": 392649, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8", "UniRef:UniRef50_A0AB32XBM8", "UniRef:UniRef90_A0AB32XBM8" ], "nt": "ATGCAAGAAATCTTAATTCCTCTCCACTCTCCCCTTTATTCAATTAAAGGAGAGACCTTCTTTGAAACTAAAGAAGCAAAGCTTTTAAAATTTGAAGAAAAATTATTCTTCATGGGACATCACACAAAATTTTTTACAGTCCCGGTTCACATACCATTTTAG", "aa": "MQEILIPLHSPLYSIKGETFFETKEAKLLKFEEKLFFMGHHTKFFTVPVHIPF", "aa_hexdigest": "80a69bfdd031766b0fe6329d2d4678bc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001F5A628", "uniref100_id": "UniRef100_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBM8", "uniref90_id": "UniRef90_A0AB32XBM8" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBM8", "uniref50_id": "UniRef50_A0AB32XBM8" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBM8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6323.4245999999985, "isoelectric_point": 6.901811027526856 }, "id": "EFPKPENHBP_733", "locus": "GCA000186005_00705" }, { "type": "cds", "sequence": "contig_1", "start": 392698, "stop": 393120, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACATTCAAACATGTATTAGATTCTAGATATTCTCTTTTAGCAAATACTCCATATACTTATTGTTTAAATATTGAAAGTTTTGTTGCTTATAAAAATCACATAATTGATGATAATGCAAAAAAACAATATAACCAAATAGTTTCTAATATTACAAATAGAATGAATTCAGCTTCAAGCAAAGAACAAGAAGCTAAAAAAATATTAGAAGAAATAAACAATAAATTACAAACGTTAAAAAATAATCCTAATACGCACTATTTTGGAAATAAAAATGTAGTAGGTCGTTCAATTGATCAAAAGCCACATATTACATTTAATATTGCTATTTATGATGAAAATCAAAAACATGATTACTACATAACAAGTACAACTAATTATCGTTTCTTCTTAAAAGATGTTAGCAAAGATATTTATTAG", "aa": "MNTFKHVLDSRYSLLANTPYTYCLNIESFVAYKNHIIDDNAKKQYNQIVSNITNRMNSASSKEQEAKKILEEINNKLQTLKNNPNTHYFGNKNVVGRSIDQKPHITFNIAIYDENQKHDYYITSTTNYRFFLKDVSKDIY", "aa_hexdigest": "2d1dd985d0c8bdff229b3ccec7af2d6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16483.280699999992, "isoelectric_point": 9.042738533020017 }, "id": "EFPKPENHBP_734", "locus": "GCA000186005_00706" }, { "type": "cds", "sequence": "contig_1", "start": 393760, "stop": 393933, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGACATGGCTTTAGATGAAGAAATGCTTGCTATTAATGATTCAAATCAAAAATTTGATGATGGTTATAGAACAGTTATGTTAGTTGGTGGATTTGCTCAATATGTTAAAGATCATCTTAATGACAAAAATTCAATTTATGCAGCTAATAGTGGTTTACAATTATGA", "aa": "MGYDMALDEEMLAINDSNQKFDDGYRTVMLVGGFAQYVKDHLNDKNSIYAANSGLQL", "aa_hexdigest": "058cc38f27be0fd0ac50babc20ee7b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6392.057600000002, "isoelectric_point": 4.265277671813965 }, "id": "EFPKPENHBP_735", "locus": "GCA000186005_00707" }, { "type": "cds", "sequence": "contig_1", "start": 394111, "stop": 394299, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAATCGTCAATATAAAGAATTAAACGAAAAATACTTCTTTGAAAGATTAAATTCATCAAATAATGCTAAAATGACTAGTTTATTAGTAGATAACTACCACAAATACTTTGTTGATTTAAATGGTAAATTACTAGGTCAAGTATCATTCAATAATGAAGATGAAAAAATTAATAAATATAGATGA", "aa": "MHNRQYKELNEKYFFERLNSSNNAKMTSLLVDNYHKYFVDLNGKLLGQVSFNNEDEKINKYR", "aa_hexdigest": "4d1107b9a47eb967b608671f251a60ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7550.3743, "isoelectric_point": 8.963442420959474 }, "id": "EFPKPENHBP_736", "locus": "GCA000186005_00708" }, { "type": "cds", "sequence": "contig_1", "start": 394678, "stop": 395037, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATAATAATCAAATAATAACAAATAAATTGCGTGAATTTGATGAACAAAATATTGAAAAATTAAGAAACTATGTTGAAGAACTTAGACAATTAGTAAATATTTATGAACATGCTTCAATAAAATTTGCAAGCACAAATTTAAATGACATTAATAAAGAAATTAGTGAAGTTGATGCTTTAATTGCAAATAGCAAAAAAGCCTATAAAACAGAAGATAAAGTAAAAAATAGCGCTGCAAACTTAAGTCAAAAAACACTTAATACTTACAATGAATTTAGAACTCAAACACTGAATAAATATAAACAATTAATGAGTCTAAATGTTACTCCTGTTGTAGCAAATGTTAGACCTTGA", "aa": "MSNNNQIITNKLREFDEQNIEKLRNYVEELRQLVNIYEHASIKFASTNLNDINKEISEVDALIANSKKAYKTEDKVKNSAANLSQKTLNTYNEFRTQTLNKYKQLMSLNVTPVVANVRP", "aa_hexdigest": "fc80d43e29d81d59d8ae6aedc9c0c765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13779.387299999977, "isoelectric_point": 9.125387001037598 }, "id": "EFPKPENHBP_737", "locus": "GCA000186005_00709" }, { "type": "cds", "sequence": "contig_1", "start": 395071, "stop": 395322, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAATTGGTAAATACCTTAAATATGTTGTTGAAGCAACAAACTTTGAAAAAGCTATTCAAGCAATTGAAAATTCATATAACAATGAACCTAGCGGATACAAAGATGTTGAAGCTTACAATAACTTCAAAAAAGAAGAACAAAACTTAGCTTCTGGAAAAACATCTATTGAAACAATACTTAACAATTTCAAAAACAAAAAACAAAGTGAATTAATTAGTGGAAATGTTCCTGAAGATGCTCAATGA", "aa": "MIKIGKYLKYVVEATNFEKAIQAIENSYNNEPSGYKDVEAYNNFKKEEQNLASGKTSIETILNNFKNKKQSELISGNVPEDAQ", "aa_hexdigest": "01ee9d39c6e4db39b23adda7938e5d25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9421.390299999994, "isoelectric_point": 5.436388206481933 }, "id": "EFPKPENHBP_738", "locus": "GCA000186005_00710" }, { "type": "cds", "sequence": "contig_1", "start": 395338, "stop": 395838, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATTCACAAAAAACTACTGCAATTATTAATTTAAAAAACCTTAAATCAGAAATTAATGCAGACTTAGCAAAACTAAAAGGCTATTCATCATTATCAAATGATCTTACTAAATCAATTGGTGATAGCTTAGTTCAATATAAAGCTTCATTACAAAAAGTAATTTATGAAATTGATGATTTAGTTCAAAAAATCAATACCATAGGTTTTGTTCAAAGTGAATATGATACTGTTTATAACAAATATAAAGATGTACCAAATAAAGACGAAAACATCATTAAATTTATTGAAAATAAAGTATTTGTTGAAACTAAATTCTTAAACAAATATGACATCAAATTAGATGAAATAACTAATCAAGAATTAAAAGATAAAGTTACAACTAAATTTAATGAATTCAAGGCAGATATAGTTAACTTCTGTTCACAAAATATTACTAATTATGATGCTGATGTTGCAACACTTACAACAAAACATCAAGCAATTCAAAATAAATGA", "aa": "MKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIYEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIKLDEITNQELKDKVTTKFNEFKADIVNFCSQNITNYDADVATLTTKHQAIQNK", "aa_hexdigest": "176459bf6b1d959e98ebadb5d1ee8a47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19061.414900000003, "isoelectric_point": 7.6834608078002935 }, "id": "EFPKPENHBP_739", "locus": "GCA000186005_00711" }, { "type": "cds", "sequence": "contig_1", "start": 396082, "stop": 397353, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6", "UniRef:UniRef90_UPI00217F41A6" ], "nt": "TTGACTACCGCTAATGATGAATTAACTGGTATTAATAAAGTTGATAACTATTCAGATTCGATTACTAAATTAAATAATCAAAAGAAAGCAGAATATCAAGAATTAATTGATCAAAATAACAATGATCTTAAAACTCTTAAAAATGCTTTAATTGAAATTAAAAATAGTAATGACTTAATTGAAATATTAATTAATGAAGTTGTCAATAAAAAATGTAACTTAATTAATGCAACTATTAAAGAATATGAAGATCAACTAGAAGCAATTAATAATTCTATTTACAGCGATTTATATGAAGACATTACAACAAGTTTACATTTAGACTTTTTAAACTCTTTAGCTATTTTAAAAAATAAATATCCAAATAATTTAAATAAAGTAATCAAACTTTCAGAAGTAAAAAGAGATTATGAAGAAATTGAAGGTAAACTTAATGATTTAAAAAATAGATTAGCCGCTACACAAAATGAAAGAAATGACATTCTTGCAGAATTAAGATTAAACTTTGACAAAATTAATTCATATGCTAATTATTCAGATGCTGATAGTAAATTGCCTTTAACTAAAGCTTTAATAAATGATGCAAATAATAATTTAGAAAATCACTTTAGTTCAATGACACTAAAATACTTAAAAGCTGAAAAATCTGCTAGTGGTCAATATACAAAAGTAATAAATGAAATAAATCAATATGAAGCAGATCTTAATGTAGCTTTACAGGAAATTATTAATGAAGTAAAAGATGCTAAATATCAAAAAATAGATGACATTATTTCAAATAATTCAAGTGTCTTTTTAGCTGACAAAGATGATTATAAAAAAACAATTGCACCAGTTAAACAAAAAGCTAATAATGGTGTTAATGACTTAAAAACTTTAATTACAACAATTAAAGATGCAAATGAAAAACTTGTAGCTATTAAAGCTCTTGGCAATAGAATTCTTACAAAAATTAATACTGAATTCAACAATAGAATTAACCGACTTAAAACTATTGCAAATGAAACTGATGGATTCTTAAGTCCTAAAGTTGCTGACGGTAAATATGAAGGTTCTATTTTGCATGGAATTAAACAATCAAATGATGAAATTATTAAAATAATTAAAACAAAAATCGATACAATAATGAACAATAATAGTAACCTTGCATACTTAGTAAAAATCGATAATTCTAGTGATAAAACTATAGAAAAATTAGATCAATTAATTGCAAGAGCAACCCAATTTAAAGATAACGCAATCACAAAAACTAATGAATATGAAAGAGCATGA", "aa": "MTTANDELTGINKVDNYSDSITKLNNQKKAEYQELIDQNNNDLKTLKNALIEIKNSNDLIEILINEVVNKKCNLINATIKEYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLAILKNKYPNNLNKVIKLSEVKRDYEEIEGKLNDLKNRLAATQNERNDILAELRLNFDKINSYANYSDADSKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERA", "aa_hexdigest": "f639a5a7d28221397cf1996faf0ae93d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 395779, "stop": 397653, "observations": { "mutation": [ 396078, 396084, 397506 ], "stop": [ 396078, 396084, 397506 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217F41A6", "query_cov": 1.0, "subject_cov": 0.4890173410404624, "identity": 0.95, "score": 751.0, "evalue": 1.02e-265, "gene_start": 1, "gene_end": 423, "reference_start": 173, "reference_end": 595, "reference_sequence": "MNDSCSSSRNSTTFDNSQALFNEVKNAANNKDQIKDVGVDNLTNNITKAEAKLKEIQNKKAEFKQENTNLRNQISELMNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQEWENKNINDLQAIKDILDGYKQSLDNYKNEAAAAYRNELKEQIQKSNNKVQKLTTANDELTGINKVKNYSDLITKLNNQKKAEYQELIDQNNNVLKTLKDALIEIKNSNNLVETLINEVVNEKCNSINAIIKKYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLATLKNKYPDNLNKVIKLSEVKRDYEEIKSKLDDLNNRLAATQNERNNILAELRLNFDKINSYANYLDADTKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERAWEEAKNENLQKLDEANGIIQFLNDKKTTSTITSEEQTALTALNTAKQNYDRIIENNIDHKSKTKIEDATNHLNDALNTARQELKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIDEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIELDEITNQELKDKVTAKFNEFKADIVNFCSQNITNYDADVATLTTKHQTIQNKWNVDSEMIKIGKYLKYVVVKQL" }, "paralog": false, "identity": 0.9567307692307693, "score": 1153.0, "evalue": 0.0, "description": "Internal stop codon at 396078, 396084, 397506. Nonsense mutation around 396078, 396084, 397506.", "psc": { "uniref90_id": "UniRef90_UPI00217F41A6", "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217F41A6", "UniRef:UniRef50_UPI00217F41A6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 47995.530699999996, "isoelectric_point": 5.4840192794799805 }, "id": "EFPKPENHBP_740", "locus": "GCA000186005_00712" }, { "type": "cds", "sequence": "contig_1", "start": 397504, "stop": 397638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATTTAAAAGTACAAGCTGATACACTTCCAAATGAATTTAATGAACACATTTTAGCAACTCATAGCTCAATAAAAACCATTCAAGAATATTATGAAAATACATATAGCAAAATTAAAAATCAAGAATGA", "aa": "MNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQE", "aa_hexdigest": "2430bc0db4efedd934b7684774efa761", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5199.6706, "isoelectric_point": 5.14724826812744 }, "id": "EFPKPENHBP_741", "locus": "GCA000186005_00713" }, { "type": "cds", "sequence": "contig_1", "start": 397771, "stop": 397866, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTGCTCAAGTTCGAGAAATTCAACAACTTTTTGATAATTCTCAAGCCTTATTTAATGAAGTTAAAAACGCTGCTAATAATAAAGATTAA", "aa": "MTAAQVREIQQLFDNSQALFNEVKNAANNKD", "aa_hexdigest": "d51460820a9d06fa5b3e406d20bbd021", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3508.827300000001, "isoelectric_point": 4.938876533508299 }, "id": "EFPKPENHBP_742", "locus": "GCA000186005_00714" }, { "type": "cds", "sequence": "contig_1", "start": 397867, "stop": 399006, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAACAATAGTTTAGCAGCTGCTAAAAATAAATTACAATCATCAATTGAAAAGGTTGAAAATCATTTAAATTCAAGAAATTCTTTAGACAATTTAGATAATTCATATTACTCAAATTTAATGCAAAATGAGAAAATTGATTACAATGAAAATAGTTCAACTGCTGCTTCCAAAAAAGATTTAAAATCTATATTAGATTCAATGAAAAATATTGTTAAAGGCGATGATGAAAATGCAATTAATCACAATGTTCAATTTGGTCAAAAATTAATTGATGCTATTGAAGAAAAAAGAACAGAAGACATAGTTGCAAAACAAAATACATTAAAAGATAAATTAAATGAATATAACGATTTAAAAGATTCATTAAAATCATTATCTTCAAAAGATTATTATGGAGCTCAAATTACAAATCTTGAAGCTAATTCAACTGCTGATGAAGATGCTGATAGAATTACAAAATTAGCACGAGCTAACTTAATTAAAGAAAGCCAAAAATCAATTGTTAATACTAAAGTTGAATTAGAAAAATTAATTGGTAATTTAACTAAAATTAAAACTGATCGTGAAGCTCTCATTCGACAAGCAAATGATCAATATAATACAAATAAAGACAAATTTGCTGAGCTTCAAAAACAACTAAATGATTTAAATATTACACCTGCTACAATAGCGGATTATAACACAAAAGTGAAACCTAAACTTGAAGCAGCAAATAATAAAAATGAATTATTAAAAGAAGTTTCTAAAGATCAAAATAATAGTAATATAACAGCTAAAATTAATGATTCTAAAGTTGCAATTCAAGATTTAGAAAATGTTGTAAACAACTATAAAACAGCTCTTAACAACAAAATTAAAGAAAATATTTCAGCAATAAAAGAATTAGTACAATACATTAGAAACAATCCAAACAATTTAACTCTTCCAGCTAATTTTGTAACCAATTTAAAAGCTGTAGAAGACAATCAAGACTCAACTGATATTGCTATTTTAAATCAAACAAAAACTCAAAGTGAAGCATTAATTAAAGAGTTAACCGATTTAATTAATACAGCAGTAGCAACTGTTAATAATAAAGCTCAAGATACAAATCAAAAAGTTGAAGAAGAAAAAGCAAAAAATGAAACATGA", "aa": "MNKNNSLAAAKNKLQSSIEKVENHLNSRNSLDNLDNSYYSNLMQNEKIDYNENSSTAASKKDLKSILDSMKNIVKGDDENAINHNVQFGQKLIDAIEEKRTEDIVAKQNTLKDKLNEYNDLKDSLKSLSSKDYYGAQITNLEANSTADEDADRITKLARANLIKESQKSIVNTKVELEKLIGNLTKIKTDREALIRQANDQYNTNKDKFAELQKQLNDLNITPATIADYNTKVKPKLEAANNKNELLKEVSKDQNNSNITAKINDSKVAIQDLENVVNNYKTALNNKIKENISAIKELVQYIRNNPNNLTLPANFVTNLKAVEDNQDSTDIAILNQTKTQSEALIKELTDLINTAVATVNNKAQDTNQKVEEEKAKNET", "aa_hexdigest": "025f079ecd49e67b322e08b1d9a69ea7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42603.91460000002, "isoelectric_point": 5.613327980041503 }, "id": "EFPKPENHBP_743", "locus": "GCA000186005_00715" }, { "type": "cds", "sequence": "contig_1", "start": 399502, "stop": 399624, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAATTAAAAATGCTATTGACAATAGTACAACTATTACTATTAAAGAATTAGAAGATTTAGTAAAAGAAAACAATGATGTATATGGAAAATATACAAGCACTAAAATTCCAGAATGA", "aa": "MEKIKNAIDNSTTITIKELEDLVKENNDVYGKYTSTKIPE", "aa_hexdigest": "e9f6967401b1ff1b986153fc06b609bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4587.117800000001, "isoelectric_point": 4.924950981140136 }, "id": "EFPKPENHBP_744", "locus": "GCA000186005_00716" }, { "type": "cds", "sequence": "contig_1", "start": 399703, "stop": 400212, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTTAATGCTGATTATGAAAATTACTTTAAATTAGATGACCATTACAAAATGGCTTTAGATATTATTATGGACAATTTAATATCTCAAATTCGTTCAGAACATATTAAAGGTATTGAAGCGAAACAAGAAGAAATTTATCGTATTTTCTATGAAAAAATGCGTGCTATTTATAATGCACAATTAACTAATGGAGTTAAATTTGTAAATGATAATATTCTAGATGCTAAAAATAGAAGAATTCAAGCATTAAAAGAATATGAAAAGGCTTTAAAAGAAGCAGAAAATTTAATCAAACATTATACAGATGAACACTATAACAATACTGATGATTTCTTCAAAACACTTGAAGAAGGCATTAAAGCTGTTAAAACTTTAAATAAAGATCGAGCAATGCTAAGTCTTGTAAAAAATACAACTATAGAATTAAAAGAAGTTATCAAAAATTCACAAGATCAATATAGAACATTCCTATTGTCTAAACTTGATGAAGCTAGAAAAAATTGA", "aa": "MQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKN", "aa_hexdigest": "42864cbd500023e603dc397f908f3820", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20091.6405, "isoelectric_point": 6.448179054260252 }, "id": "EFPKPENHBP_745", "locus": "GCA000186005_00717" }, { "type": "cds", "sequence": "contig_1", "start": 400258, "stop": 402243, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80", "UniRef:UniRef90_C4XG80" ], "nt": "ATGTCTGATGATGATTTTCTAAAAAATTTAGAAATTAAAGGACAAAATGTAAGTGAAATTTATGCTAGTGATGCAAAAATTACAAATGTTGAATACAAAAAAGATTCTAATTATGAAATTAATGTTTCTCTAGAAGGATCAAATGTTCTAAAATCAACTGCATACTACAAAATTGAAATTACAAATAAAAATACAAAAGTTAAAAAGTCAAAAATTGTTCCAATAAGTGGTTTCAAGAAGTTTGATGAAAAAAGTACTATTTTAAAAGAAAAAGAGCTATTTGAAGATAAATCTAAATTAAAAGAATATTTAGATAATATGCCTAAAAATGATTCTGAAAATCTTAAAAAATATATTTCTAAAAATAAACATTTTATTTACAACGAAATACCAAGTTCATTAAAAGCTTCATTTAAAGGTTATTCTTTCATTCAATCTAATGGTCAAGATGCTGTTAGAGTTGTTTTCGTTTTAAATGGTCATATTACAAATGATAATCTCGAAAATAATATAGTTAAAAAAGCAAATAAAGAATATAAAAATAATGAAAAAACTTTTGAATTTTACTTAAGTAATTTAGTAAAACAAGGAACATCAAGTTCAGAAATTCAAGCAAGAAATAACTTAAGAGATTTAAAAGAAAAATACGAAACTCTTAAAAAAGAAGCTGAAGTAGCTAAATCGAAAGTTACAGTTGCTATTGTTGCTGAATATGAAAATGTTTTAGTTTCAATTACTAAAAATATTAATGAAATCGACAAAATTTTAAATGATGCAACAATTAAAGCAGCAGATCATGTTGATACATTAAAAGTTAATTATGAAAAAGACTTATTAGATAAAAATACTGTAGATTTAAAAGCAACAATTAATGATGCTGAAGTTACAAGAGATAACTATAAAGCTAAAGATGAAACAAATTATAACAATCCAATAATTGAATTTAACAAAGCTATTGATCAAGCAAAAATTAAACTTGCTTCAACAAATATTAAAGAAATTAATGATGAAATTTCTAACTTAAAAACTAAAAAAGAAGAGTTTATTAATGCTTTAAAAACTATCTTAACTGACACACAAAGTGATTCAAAAACTAAATTGTTAGAATCAAGCGATTACAAATATTTGATCGATGATATTAAAGCTCGTAAAGCTACTTTTGATGCTACACTTAATGAAGATAATATTCTAAGTGAAATTGAAAACTTAGAAGTTTTAGTTCAAAATACTCAAACAATTGATGCTATTACACCAAAAGCAATAATTGAAGCTAATAATAAACTTCTAGCTTCCTTACTAGCCTATAAAACAGCCTTAGAAGCAAAATTACACAAAATAAAACAAGATGTAATAGACTCAACTTATGACAAAGATTCATTGTCACAATTTATCGAATATTTAATTAAAAAAATTAAAACAACAAATGAAAGCAAAGCTTTAGTTAATAAACAAATAGATTTATTTAAAGCTTATTTAACTACACCAGGTTTTGTTCAAAAATATAATTCTTTAGAAAAAGATTTTGATAAATATGAAGCAATTTTTAATGAAAATATAAATAAAGCTAATGAACTTATCGCAACTCTAGATGCAGGATTAGATCAATATTCAAATTCAAAAGATAAATATAGTGAAATCAAAACTAAAGTTGACTCACTTTTAGCAGAACAAGCAACTCGTTTAAGCTCAATTGGAACTCAAATTGTTAATAGTGCACTTATTGATTTTGATAATTATGTTCAAAAGCAAAAAGCTTTAATCAATACTTCACGTAATAACTACAAAACAATTGTGACAAATGTTTACAATTTATTCAAAGATTCAGAAATCTACAAACAATATATAGATGATAATAAAGCGAAACTTACAAACTCAACTATTGATGTTAAAACTGCTTTTGAATTATTAAAAACTTTATATACAAAAGATAATGAAATGAATCAAAAAGTTGCAGCTCACTCAAACTTAGCAACTAATAGCTACTGA", "aa": "MSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTVAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSY", "aa_hexdigest": "db7503173919034919add0ad609e86a5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 399955, "stop": 402363, "observations": { "mutation": [ 400260, 402321 ], "stop": [ 400260, 402321 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG80", "query_cov": 1.0, "subject_cov": 0.7099892588614393, "identity": 0.998, "score": 1217.0, "evalue": 0.0, "gene_start": 1, "gene_end": 661, "reference_start": 41, "reference_end": 701, "reference_sequence": "MSTKTISKATKGLGWAIATATITGSILLTIIAIKTQNTKKMSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTGAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSYWNSLLDGDLKNNFTIPMQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKNWQIYSNSINGPEHQDVFPKTIKLINESWKKLKMLLTIVQLLLLKN" }, "paralog": false, "identity": 0.9962593516209476, "score": 1537.0, "evalue": 0.0, "description": "Internal stop codon at 400260, 402321. Nonsense mutation around 400260, 402321.", "psc": { "uniref90_id": "UniRef90_C4XG80", "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG80", "UniRef:UniRef50_C4XG80" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 75411.24320000027, "isoelectric_point": 6.638475990295411 }, "id": "EFPKPENHBP_746", "locus": "GCA000186005_00718" }, { "type": "cds", "sequence": "contig_1", "start": 402628, "stop": 402960, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354585.1", "SO:0001217", "UniParc:UPI0001A8C1FC", "UniRef:UniRef100_C4XG79", "UniRef:UniRef50_C4XG79", "UniRef:UniRef90_C4XG79" ], "nt": "ATGTTTTCAATAGTTGCTAATGATAAATTTTTGGGCCTAGCAGAAAGTGCCAAAAAATTTAAATATGTATTACAAGATTTAAAAAATAAAGGCGCTACTAACTACGAATGTGTTGAACGAAATATTTTAAAAACCGTATTTAATAGTAAAAAAACACAAATATTTTTATATCAAGAAAATAATAATTTGAGAGCAGAAAATATTGTTAATTTCAATCTTAAATATTTTAGCAATAGTGTTAAAAATGAATTAAATGATTCTTTACTTTCTAATGATGATTTAATAAAATTAAGCTTGAAAAAGCTTATGTTGAAATTACCAAAATATCTTTAA", "aa": "MFSIVANDKFLGLAESAKKFKYVLQDLKNKGATNYECVERNILKTVFNSKKTQIFLYQENNNLRAENIVNFNLKYFSNSVKNELNDSLLSNDDLIKLSLKKLMLKLPKYL", "aa_hexdigest": "8efe922e4c33b3d09deb2d5d138b68db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1FC", "ncbi_nrp_id": "WP_013354585.1", "uniref100_id": "UniRef100_C4XG79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1FC", "RefSeq:WP_013354585.1", "UniRef:UniRef100_C4XG79" ] }, "ips": { "uniref100_id": "UniRef100_C4XG79", "uniref90_id": "UniRef90_C4XG79" }, "psc": { "uniref90_id": "UniRef90_C4XG79", "uniref50_id": "UniRef50_C4XG79" }, "pscc": { "uniref50_id": "UniRef50_C4XG79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG79" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12802.756799999983, "isoelectric_point": 9.520449256896974 }, "id": "EFPKPENHBP_747", "locus": "GCA000186005_00719" }, { "type": "cds", "sequence": "contig_1", "start": 403424, "stop": 403654, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354584.1", "SO:0001217", "UniParc:UPI0001E330CC", "UniRef:UniRef100_A0AB32XBB6", "UniRef:UniRef50_A0AB32XBB6", "UniRef:UniRef90_A0AB32XBB6" ], "nt": "TTGAAAAGCAATGCTAAAGTTAAATCAAAAATTAAACAAGTAAAAATTAATAATTTCAAAATAGTTAAATTAACAAAATCTTTTGAAGAATTATTTTTTGGAGTTAATGATTCTTCAAAATTATCGGCCAATAACGTTATAACTTTAGATCAATATGTTGATAATTTAAACTTATTTCAGAGCAAAATTAATCTTAATTTAAATTTAATTAATAAAGAACTAAAAATTTAA", "aa": "MKSNAKVKSKIKQVKINNFKIVKLTKSFEELFFGVNDSSKLSANNVITLDQYVDNLNLFQSKINLNLNLINKELKI", "aa_hexdigest": "a839b59558589508e25b624123782c0d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330CC", "ncbi_nrp_id": "WP_013354584.1", "uniref100_id": "UniRef100_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330CC", "RefSeq:WP_013354584.1", "UniRef:UniRef100_A0AB32XBB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBB6", "uniref90_id": "UniRef90_A0AB32XBB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBB6", "uniref50_id": "UniRef50_A0AB32XBB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBB6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8730.137599999995, "isoelectric_point": 9.875218772888182 }, "id": "EFPKPENHBP_748", "locus": "GCA000186005_00720" }, { "type": "cds", "sequence": "contig_1", "start": 404309, "stop": 404866, "strand": "-", "frame": 3, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_749", "locus": "GCA000186005_00721" }, { "type": "cds", "sequence": "contig_1", "start": 404932, "stop": 405222, "strand": "-", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_750", "locus": "GCA000186005_00722" }, { "type": "cds", "sequence": "contig_1", "start": 406016, "stop": 406762, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0", "UniRef:UniRef90_C4XFB0" ], "nt": "ATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTTACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGA", "aa": "MYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNFTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLE", "aa_hexdigest": "db07f0014a89237cf6973524632b4509", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 405764, "stop": 406936, "observations": { "mutation": [ 405931, 406018, 406882 ], "stop": [ 405931, 406018, 406882 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB0", "query_cov": 1.0, "subject_cov": 0.6358974358974359, "identity": 0.996, "score": 489.0, "evalue": 1.43e-172, "gene_start": 1, "gene_end": 248, "reference_start": 59, "reference_end": 306, "reference_sequence": "MIMEGAVKNKMSKTLADNWNAQAEVFKNSEERKIKYNVERKIERDIFIWGETVRIIYYMYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLEWITKQEKNKTVYANAKKFKKYYKENREYIWNSVLVKNYFGGCAETVVGHDLKRFCSKKFATFSLKTIQLSIAKNMETKANLFFI" }, "paralog": false, "identity": 0.9871794871794872, "score": 766.0, "evalue": 2.1e-284, "description": "Internal stop codon at 405931, 406018, 406882. Nonsense mutation around 405931, 406018, 406882.", "psc": { "uniref90_id": "UniRef90_C4XFB0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB0", "UniRef:UniRef50_C4XFB0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0" ], "product": "ISLre2 family transposase" } }, "id": "EFPKPENHBP_751", "locus": "GCA000186005_00723" }, { "type": "cds", "sequence": "contig_1", "start": 407179, "stop": 407628, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_C4XFA9" ], "nt": "TTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAAAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGA", "aa": "MLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHND", "aa_hexdigest": "d11fea40fd11324cc7c0bad85465fa78", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 406978, "stop": 407919, "observations": { "mutation": [ 407181, 407685, 407889 ], "stop": [ 407181, 407685, 407889 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA9", "query_cov": 1.0, "subject_cov": 0.476038338658147, "identity": 0.993, "score": 280.0, "evalue": 6.88e-93, "gene_start": 1, "gene_end": 149, "reference_start": 98, "reference_end": 246, "reference_sequence": "MYNFYMSLELWRPLKITNPLLEDIFHLNIDTKFKIHPLIIFENTKNKTYYCIRLQTARKSSSKYNLIIDNSSYQTNEYWKNHESVVVTKDIFIIDKELLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHNDWKDLDKTKDNVNIYSFENIGFLSKSSKSESSYIDPYDNKNIEKAKDKVAKEIPIKESKTDKDKTKLK" }, "paralog": false, "identity": 0.987220447284345, "score": 610.0, "evalue": 1.67e-225, "description": "Internal stop codon at 407181, 407685, 407889. Nonsense mutation around 407181, 407685, 407889.", "psc": { "uniref90_id": "UniRef90_C4XFA9", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA9", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17230.18999999998, "isoelectric_point": 8.557549476623539 }, "id": "EFPKPENHBP_752", "locus": "GCA000186005_00724" }, { "type": "cds", "sequence": "contig_1", "start": 409068, "stop": 409217, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTGCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAA", "aa": "MNKKGFKAGKSKKQVAIKKFKEIQIYKKDRIKAKPVVLSDEEINKLFAK", "aa_hexdigest": "d9f0168a356d3bfa98a930622ed41f2c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5693.836700000001, "isoelectric_point": 10.22006015777588 }, "id": "EFPKPENHBP_753", "locus": "GCA000186005_00725" }, { "type": "cds", "sequence": "contig_1", "start": 409988, "stop": 410326, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTAAAGATGGTATTGATATTAAGATAA", "aa": "MRGTGKYINHSITFNLKNSAYHVYYPPIDLSSLDIQKHFEIEMGKNISNDSKELKEKIRNKLIGLIEEQFNLYKNKTGIEMLLNKDIKVEGLDNVLDLLVQGNKLKMVLILR", "aa_hexdigest": "2dbf337af9a77bdf4cea55b81071f60a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12992.037199999986, "isoelectric_point": 9.072393989562986 }, "id": "EFPKPENHBP_754", "locus": "GCA000186005_00726" }, { "type": "cds", "sequence": "contig_1", "start": 410429, "stop": 410596, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGA", "aa": "MNYGDETNEAIKENYRRQVKEIVLNFVKRNIANSAKLKAIGITENEINGGWVIAN", "aa_hexdigest": "81fb4d5b440c05c797eb70b5ddfcd8b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.0062, "isoelectric_point": 8.06539783477783 }, "id": "EFPKPENHBP_755", "locus": "GCA000186005_00727" }, { "type": "cds", "sequence": "contig_1", "start": 410621, "stop": 410719, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGA", "aa": "MISLYKNFVDQASKFNVETGNEYENIFKDKIT", "aa_hexdigest": "1f15fa2f5e0a93065b5e0a705db0eb3e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3787.208700000001, "isoelectric_point": 4.93575038909912 }, "id": "EFPKPENHBP_756", "locus": "GCA000186005_00728" }, { "type": "cds", "sequence": "contig_1", "start": 410801, "stop": 411265, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGA", "aa": "MFSDELKEQLRFVYPNIKITTDKADLGIYAEANVLGKGALRNLVVDKVLESSSRYYKCLLFRKENDKYVEIGRKEQKLIEIPVNNGTSEYSYMSEEGIWLLFTVNEKSISNVNLVLIDAKSEPKSFFLDSIENKIEKINNQSEDYFEAFLDGGN", "aa_hexdigest": "14f3e53a5efaf9d6cffd2a93f9497e12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17724.8618, "isoelectric_point": 4.860722923278809 }, "id": "EFPKPENHBP_757", "locus": "GCA000186005_00729" }, { "type": "cds", "sequence": "contig_1", "start": 411278, "stop": 411421, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGA", "aa": "MDQKQIISPYMLDSKGKELKDKEGKLIVNKNYDPSIDKETGTKKQIV", "aa_hexdigest": "819168f07f3459a3efeb395e0df65970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5396.195300000002, "isoelectric_point": 8.96376476287842 }, "id": "EFPKPENHBP_758", "locus": "GCA000186005_00730" }, { "type": "cds", "sequence": "contig_1", "start": 411437, "stop": 411712, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTAGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGA", "aa": "MVNKQKVSVLNRIFEKKLIDERVNVNDASKIPFDKNSGSGNGEGDVAKNEYLVEVYRYKKGSNNSREPEEKYVVIYEVIGNQFKQNIKYYA", "aa_hexdigest": "237428c664dc7c59657ea21ba204f35c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10538.7424, "isoelectric_point": 9.071555900573731 }, "id": "EFPKPENHBP_759", "locus": "GCA000186005_00731" }, { "type": "cds", "sequence": "contig_1", "start": 411788, "stop": 411988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGA", "aa": "MYKRLSGASLASRLTISTSITNPDGSISQIEAKTKANGGDLKNLIKRDEPERVSNKELEKDFVGKP", "aa_hexdigest": "e3d98ceff45638283a1d2f30053658e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.081999999999, "isoelectric_point": 9.453788948059085 }, "id": "EFPKPENHBP_760", "locus": "GCA000186005_00732" }, { "type": "cds", "sequence": "contig_1", "start": 412115, "stop": 412465, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTGACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGTTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGA", "aa": "MNRFEYGVNKKIDQNHYSIDLLSDTLGDLTTSVDGVFKDNGGKSNIEHLNKIFDKEYEFEYASGIKVKYKYDYKIKNEVVGEKNKIDVYIKVLSVKIDDKEIIDKDLDKILNNIED", "aa_hexdigest": "eec9c8672b54a060b72b4d66c421f750", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13503.051099999984, "isoelectric_point": 5.077279472351074 }, "id": "EFPKPENHBP_761", "locus": "GCA000186005_00733" }, { "type": "cds", "sequence": "contig_1", "start": 412508, "stop": 412759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAGTAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGA", "aa": "MEEHKGASFTNLFAEYKVSQKTLEFLNNLETDKDKRVYSGLTDDKNKMFLWGLHDFDCEIEGMYDVRYYSNKVLNNLEYAKVG", "aa_hexdigest": "e962835ba7f8c5dbf29977ecf123985e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9799.882199999998, "isoelectric_point": 5.06073932647705 }, "id": "EFPKPENHBP_762", "locus": "GCA000186005_00734" }, { "type": "cds", "sequence": "contig_1", "start": 412871, "stop": 413332, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGA", "aa": "MHAEARAKNEEKMNKKKMLKLIPLSVLSIAIPSVLNLSLNTNKKLLGYGKDFKAPEEKHKIDTNSLFSNFTSMEDDEIAKNERLQNRYQVKWSQLCDKGLLYAQDQWVGYHKTGPVKNKITSIVSRPTLGSFEVRYEKAYYTNPSNHNSYGWF", "aa_hexdigest": "547e92dcc2bb3430ecf9e1292e14eeb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17690.019999999993, "isoelectric_point": 9.477900123596193 }, "id": "EFPKPENHBP_763", "locus": "GCA000186005_00735" }, { "type": "cds", "sequence": "contig_1", "start": 413378, "stop": 413773, "strand": "-", "frame": 3, "gene": null, "product": "ICEF Integrative Conjugal Element-II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7", "UniRef:UniRef90_C4XFA7" ], "nt": "TTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAA", "aa": "MTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK", "aa_hexdigest": "1e201de3510ce021ade96f9646eff4b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 413378, "stop": 414064, "observations": { "mutation": [ 413782, 414055 ], "stop": [ 413782, 414055 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA7", "query_cov": 1.0, "subject_cov": 0.5745614035087719, "identity": 0.992, "score": 242.0, "evalue": 2.31e-79, "gene_start": 1, "gene_end": 131, "reference_start": 98, "reference_end": 228, "reference_sequence": "MKTWEISQENKKGDKLYVKSSRASKNGSKCISTRLSEPDYVALYERWVKRIKQSGSTPYVELKDIIRNALIKQEKEEIAKDFKNDLFYALRKVSWASLTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK" }, "paralog": false, "identity": 0.9912280701754386, "score": 441.0, "evalue": 2.72e-160, "description": "Internal stop codon at 413782, 414055. Nonsense mutation around 413782, 414055.", "psc": { "uniref90_id": "UniRef90_C4XFA7", "product": "ICEF Integrative Conjugal Element-II", "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA7", "UniRef:UniRef50_C4XFA7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7" ], "product": "ICEF Integrative Conjugal Element-II" } }, "id": "EFPKPENHBP_764", "locus": "GCA000186005_00736" }, { "type": "cds", "sequence": "contig_1", "start": 414045, "stop": 414851, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ], "nt": "ATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAACATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MDDLIKQMKKFVAPKDHHDKEGYTKKVLDMAELLEGKFGRYGTLGALYNGHTNINIENDLVVFKTSNLIDTEGSVNARIGIMVLISLVNGFIFDNAFNNKKRINEYKEKNKIRLISKKEIQKLTRYCSFCIDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "fd4e47e07cfb58f2a4c9ea07298295fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F2982C0", "query_cov": 0.9776119402985075, "subject_cov": 0.9225352112676056, "identity": 0.622, "score": 325.0, "evalue": 4.81e-109, "valid": false, "uniref50_id": "UniRef50_UPI001F2982C0" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2982C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30615.79740000003, "isoelectric_point": 8.78963565826416 }, "id": "EFPKPENHBP_765", "locus": "GCA000186005_00737" }, { "type": "cds", "sequence": "contig_1", "start": 414858, "stop": 415304, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGA", "aa": "MFSLTSSRANHNVMILGTSGMGKSTLTKKILTSSLAAGNIAIIIDPQAEYVKWANKMGGQIVDLGSGDGTVINPLQVKSFIAREEDDANVNFNTLINNHLLWLEKFFSFIFDDMNEMKKNILQLEIIKLYQSWGIYRLKEVKELKNDG", "aa_hexdigest": "7bdeadcb03d81e2d1d7547110e406fd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 49, "aa_cov": 0.3310810810810811, "hmm_cov": 1.0, "evalue": 6.643206648327057e-08, "score": 28.243488311767578, "start": 9, "stop": 57 }, { "id": "PF01935.23", "name": "DUF87", "length": 108, "aa_cov": 0.722972972972973, "hmm_cov": 0.7777777777777778, "evalue": 2.353833018396146e-10, "score": 37.21625900268555, "start": 12, "stop": 118 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 76, "aa_cov": 0.5135135135135135, "hmm_cov": 0.881578947368421, "evalue": 2.441558326642471e-08, "score": 29.528656005859375, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 16628.021899999992, "isoelectric_point": 6.732430839538575 }, "id": "EFPKPENHBP_766", "locus": "GCA000186005_00738" }, { "type": "cds", "sequence": "contig_1", "start": 415308, "stop": 415721, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGA", "aa": "MDAGNLDNSIINRFGSALEEESIAVMIDQIQADNFKLFDSNIFVLAEAENAEELKQNNLKIKNGFTRSNFGINDLTFRQFEGFLDMSNYPLSKLDREYYQITSINNAIGYPLKNLPLNDGNNLLLGNEVGGNDSPIV", "aa_hexdigest": "60571d7151425553be189582d3abe5bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15326.890099999975, "isoelectric_point": 4.264652442932129 }, "id": "EFPKPENHBP_767", "locus": "GCA000186005_00739" }, { "type": "cds", "sequence": "contig_1", "start": 415827, "stop": 416174, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGTTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGA", "aa": "MIETFYLILNAPTLEELDECYSTFIQKVEDYEVISYEKLSGFELLSFLNKLNHFNKSEEDIETFLNESDIYESEYSLDRLFKYEKVEFKSNSLKIDDKLYSVKCAGNIPIRLNNE", "aa_hexdigest": "a5b47dac5558579fb47e32e7c5595410", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13650.17029999998, "isoelectric_point": 4.459382820129394 }, "id": "EFPKPENHBP_768", "locus": "GCA000186005_00740" }, { "type": "cds", "sequence": "contig_1", "start": 416229, "stop": 416393, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGACGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGA", "aa": "MVLIENFARILDSFKYKFSIVKLDEQIDYRRNLNYINTIQSKKMLNSGGETKEF", "aa_hexdigest": "7eabf1fafeeaa9103dfce5aa5be32540", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.395699999999, "isoelectric_point": 9.098954963684083 }, "id": "EFPKPENHBP_769", "locus": "GCA000186005_00741" }, { "type": "cds", "sequence": "contig_1", "start": 416415, "stop": 416618, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTTAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGGAAAAATATATGA", "aa": "MFKFKAMPKKYSNVVDDKNVRDSSDLNPYSTLVENDVVKNKSFLNKLFQKSEHSNYFSVFKLGGKNI", "aa_hexdigest": "6dcc1c77a31225f74ee1d7d21c4c74f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7780.800799999997, "isoelectric_point": 9.601872825622557 }, "id": "EFPKPENHBP_770", "locus": "GCA000186005_00742" }, { "type": "cds", "sequence": "contig_1", "start": 417145, "stop": 417585, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCACCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAA", "aa": "MNICVKNRFTAPKKAKSICSPRKNTPTDNIFPTYLSFHILTFLPSSSKLKGKIIGNIKYKKNNGNIFPMASIHPKTSGIAINTAPIIDRNAAVIKMDAQLPMFKCSVFFAKATKLPKLISKLIVKPKGFVVAGIKISLTSPANFGG", "aa_hexdigest": "52b82da9c6fa93967d593309ac13844f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15892.917899999971, "isoelectric_point": 10.454209327697754 }, "id": "EFPKPENHBP_771", "locus": "GCA000186005_00743" }, { "type": "cds", "sequence": "contig_1", "start": 417438, "stop": 417749, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGA", "aa": "MRSALVFSVKSLFILLAFQITILLFNLFVQKIITLMIGEGNDIVVAFINGLHRLFYPPKLAGDVKDILIPATTNPFGFTINLDISFGSFVALAKKTEHLNIGN", "aa_hexdigest": "1f530bd3ff05364bbc363b2280c88bc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11381.43519999998, "isoelectric_point": 9.223765754699706 }, "id": "EFPKPENHBP_772", "locus": "GCA000186005_00744" }, { "type": "cds", "sequence": "contig_1", "start": 417816, "stop": 418064, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGA", "aa": "MSGFFTRVMEIFKYGPTYVLYLLLFFVSLIVFILLAILLSVVKLATFDLLNFVIFGIMPRQNILDHQSPYMYVRFAIVSAVI", "aa_hexdigest": "594dbf9addb063641ab0662d22f90068", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9502.483199999995, "isoelectric_point": 9.222605323791505 }, "id": "EFPKPENHBP_773", "locus": "GCA000186005_00745" }, { "type": "cds", "sequence": "contig_1", "start": 418154, "stop": 418345, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0395 family pilin-like conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4", "UniRef:UniRef90_C4XFA4" ], "nt": "ATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAACAAAACAATAAAAAGATTTTGTGA", "aa": "MKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKIL", "aa_hexdigest": "5087b9cbe03a20493b3e281279e3d943", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 418064, "stop": 418351, "observations": { "mutation": [ 418120, 418156 ], "stop": [ 418120, 418156 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA4", "query_cov": 1.0, "subject_cov": 0.6631578947368421, "identity": 1.0, "score": 117.0, "evalue": 4.48e-33, "gene_start": 1, "gene_end": 63, "reference_start": 3, "reference_end": 65, "reference_sequence": "MKMKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKILWAGIVLILVIVGWGIFELVISIQASGGKTS" }, "paralog": false, "identity": 0.968421052631579, "score": 172.0, "evalue": 2.82e-57, "description": "Internal stop codon at 418120, 418156. Nonsense mutation around 418120, 418156.", "psc": { "uniref90_id": "UniRef90_C4XFA4", "product": "Mbov_0395 family pilin-like conjugal transfer protein", "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA4", "UniRef:UniRef50_C4XFA4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4" ] } }, "id": "EFPKPENHBP_774", "locus": "GCA000186005_00746" }, { "type": "cds", "sequence": "contig_1", "start": 418517, "stop": 418723, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "EFPKPENHBP_775", "locus": "GCA000186005_00747" }, { "type": "cds", "sequence": "contig_1", "start": 418727, "stop": 419092, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTCAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACCAATATATAGAAATTATTTTAAAGAGCACTTGGATAAAGCAACAAATGCATTGCTTAATGTTCTTAAATATGGAGCAGTCTTTGGTGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGTGATAGTCAATTTGCTGATTATGAATATAGCTACGTGTGGGCGTATAGAACACTATATGATGTGCTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEPIYRNYFKEHLDKATNALLNVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDSQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "bda667bc8220b44beca36b09b39af68d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14106.255799999994, "isoelectric_point": 6.592834281921387 }, "id": "EFPKPENHBP_776", "locus": "GCA000186005_00748" }, { "type": "cds", "sequence": "contig_1", "start": 419117, "stop": 419443, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAGAACACTATCACTATTATTTTGATATGGACCTTGATAATTACTATAAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATATGGAATAATTATTTCGACTTAGGAAGCGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAAGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVREHYHYYFDMDLDNYYKQFINDFALKGTSKTFKDIAIDGLYKQGIWNNYFDLGSDYSKWFYLAFTRSNVEKYHESFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "a0ba9ed63962212ed2112a9f49446b5d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.521199999988, "isoelectric_point": 5.049883079528808 }, "id": "EFPKPENHBP_777", "locus": "GCA000186005_00749" }, { "type": "cds", "sequence": "contig_1", "start": 419444, "stop": 419707, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGACCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGPQKDVFAYKDSYHGNK", "aa_hexdigest": "14665cc564c7c5abfd5e5a988a3ba75f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10213.393699999997, "isoelectric_point": 4.955473518371581 }, "id": "EFPKPENHBP_778", "locus": "GCA000186005_00750" }, { "type": "cds", "sequence": "contig_1", "start": 419723, "stop": 420028, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCACAAGTATTGCATAATGAGCAAAAACATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MKKSKKFMKFMAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKHDADVYRNDN", "aa_hexdigest": "97c66c7a439d072574bce14a5b39a997", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11515.666999999992, "isoelectric_point": 6.024785423278809 }, "id": "EFPKPENHBP_779", "locus": "GCA000186005_00751" }, { "type": "cds", "sequence": "contig_1", "start": 420109, "stop": 420306, "strand": "-", "frame": 1, "gene": null, "product": "ICEF-IA ORF13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945", "UniRef:UniRef90_Q8G945" ], "nt": "ATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGA", "aa": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVY", "aa_hexdigest": "37a7ed9202ae80cc53604b2722443644", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 420052, "stop": 420306, "observations": { "mutation": [ 420087, 420111 ], "stop": [ 420087, 420111 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8G945", "query_cov": 1.0, "subject_cov": 0.7738095238095238, "identity": 1.0, "score": 122.0, "evalue": 3.68e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVYWVKKRIEYWKQSKKNSDLH" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.29e-53, "description": "Internal stop codon at 420087, 420111. Nonsense mutation around 420087, 420111.", "psc": { "uniref90_id": "UniRef90_Q8G945", "product": "ICEF-IA ORF13", "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8G945", "UniRef:UniRef50_Q8G945" ] }, "pscc": { "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945" ], "product": "ICEF-IA ORF13" } }, "id": "EFPKPENHBP_780", "locus": "GCA000186005_00752" }, { "type": "cds", "sequence": "contig_1", "start": 420306, "stop": 420857, "strand": "-", "frame": 2, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "RefSeq:WP_013526819.1", "SO:0001217", "UniParc:UPI00000C02A9", "UniRef:UniRef100_Q8GCN6", "UniRef:UniRef50_C4XFA2", "UniRef:UniRef90_C4XFA2" ], "nt": "ATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAATCAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAA", "aa": "MNKVILTGRVVSEDYFKAKTTSGKAVLGFVIAVNNEYGGEPDFLNITAFGLQASNFEKYNKKGDLVEVIGKIKRNAYVDRDNHKQYKMEIVADNIIYSAKKVDRNAEGNDEDIIYKDESKLPKEEKEKVVKARLKKKDEMMEPFKQYIGMDPLCQSVYDKYEEEAFNKDVEVKDETKKHGQTL", "aa_hexdigest": "70632c095dd94960fc10b7a413bafc76", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02A9", "ncbi_nrp_id": "WP_013526819.1", "uniref100_id": "UniRef100_Q8GCN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A9", "RefSeq:WP_013526819.1", "UniRef:UniRef100_Q8GCN6" ] }, "ips": { "uniref100_id": "UniRef100_Q8GCN6", "uniref90_id": "UniRef90_C4XFA2" }, "psc": { "uniref90_id": "UniRef90_C4XFA2", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_C4XFA2" }, "pscc": { "uniref50_id": "UniRef50_C4XFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA2" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "EFPKPENHBP_781", "locus": "GCA000186005_00753" }, { "type": "cds", "sequence": "contig_1", "start": 420867, "stop": 421583, "strand": "-", "frame": 2, "gene": null, "product": "Mbov_0392 family ICE element protein", "db_xrefs": [ "RefSeq:WP_013526820.1", "SO:0001217", "UniParc:UPI0001F5A76C", "UniRef:UniRef100_A0AB32XBD2", "UniRef:UniRef50_C4XFA1", "UniRef:UniRef90_C4XFA1" ], "nt": "ATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGAATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAA", "aa": "MIEKVFCFKDLYYLTQYALSDISDEEVVAKFNYLAKNIKNNTMRNKYKKQVLSIVDKMSNVDDDINQKIFDKLFYVEDTNEKNLEQYITLLNICNVEDLENIENNSLDLKFSFNDFFGENGYIESEYLFDLVDEDLKQNGINSKYIYSLGDLSRIDTEYVYIDDYLNFHPEDIDDIKKEYIENLASLKLDEIIDLNNKESIDANNKVEITDKTVKEAQKHVAGIVEKESETAKVKNTK", "aa_hexdigest": "1d5dae40ecb08a4a7d0e659a5dbbb4b8", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F5A76C", "ncbi_nrp_id": "WP_013526820.1", "uniref100_id": "UniRef100_A0AB32XBD2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A76C", "RefSeq:WP_013526820.1", "UniRef:UniRef100_A0AB32XBD2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBD2", "uniref90_id": "UniRef90_C4XFA1" }, "psc": { "uniref90_id": "UniRef90_C4XFA1", "product": "Mbov_0392 family ICE element protein", "uniref50_id": "UniRef50_C4XFA1" }, "pscc": { "uniref50_id": "UniRef50_C4XFA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA1" ] }, "genes": [], "id": "EFPKPENHBP_782", "locus": "GCA000186005_00754" }, { "type": "cds", "sequence": "contig_1", "start": 421596, "stop": 421760, "strand": "-", "frame": 2, "gene": null, "product": "ICEF-IA ORF10", "db_xrefs": [ "RefSeq:WP_013526821.1", "SO:0001217", "UniParc:UPI00000C02A7", "UniRef:UniRef100_Q8GCN8", "UniRef:UniRef50_Q8GCN8", "UniRef:UniRef90_Q8GCN8" ], "nt": "ATGATTAGTAAAAGAAAAGTAGATGAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAG", "aa": "MISKRKVDEDLKQNGIKSTLAKKYLEEFKKMEPEKYEKFRKESNKKICNKLFAK", "aa_hexdigest": "745e3710917449f05cf73c983a39c512", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02A7", "ncbi_nrp_id": "WP_013526821.1", "uniref100_id": "UniRef100_Q8GCN8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A7", "RefSeq:WP_013526821.1", "UniRef:UniRef100_Q8GCN8" ] }, "ips": { "uniref100_id": "UniRef100_Q8GCN8", "uniref90_id": "UniRef90_Q8GCN8" }, "psc": { "uniref90_id": "UniRef90_Q8GCN8", "product": "ICEF-IA ORF10", "uniref50_id": "UniRef50_Q8GCN8" }, "pscc": { "uniref50_id": "UniRef50_Q8GCN8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8GCN8" ], "product": "ICEF-IA ORF10" }, "genes": [], "id": "EFPKPENHBP_783", "locus": "GCA000186005_00755" }, { "type": "cds", "sequence": "contig_1", "start": 422197, "stop": 422697, "strand": "-", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAGCAGAACTTAAAAGCGCCTTAACAAATGACCCTACATTAAAACTTTTAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGACAAATTTATTCAATATTGTGCTTTAAGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "def91864d865c35b1e877f7777dd1f12", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 421894, "stop": 422697, "observations": { "mutation": [ 421950, 422076, 422199 ], "stop": [ 421950, 422076, 422199 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 1.0, "score": 335.0, "evalue": 1.55e-114, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9887640449438202, "score": 528.0, "evalue": 2.56e-193, "description": "Internal stop codon at 421950, 422076, 422199. Nonsense mutation around 421950, 422076, 422199.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "EFPKPENHBP_784", "locus": "GCA000186005_00756" }, { "type": "cds", "sequence": "contig_1", "start": 422687, "stop": 422875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526823.1", "SO:0001217", "UniParc:UPI00000C02A5", "UniRef:UniRef100_C4XE09", "UniRef:UniRef50_C4XE09", "UniRef:UniRef90_C4XE09" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAGATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTAAAAACAATAGGAGAAAAATATGGAATATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEELKTIGEKYGI", "aa_hexdigest": "f1ce7457fea8a13f6a0f5878f8f91e20", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A5", "ncbi_nrp_id": "WP_013526823.1", "uniref100_id": "UniRef100_C4XE09", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A5", "RefSeq:WP_013526823.1", "UniRef:UniRef100_C4XE09" ] }, "ips": { "uniref100_id": "UniRef100_C4XE09", "uniref90_id": "UniRef90_C4XE09" }, "psc": { "uniref90_id": "UniRef90_C4XE09", "uniref50_id": "UniRef50_C4XE09" }, "pscc": { "uniref50_id": "UniRef50_C4XE09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE09" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7209.3438000000015, "isoelectric_point": 9.331879234313966 }, "id": "EFPKPENHBP_785", "locus": "GCA000186005_00757" }, { "type": "cds", "sequence": "contig_1", "start": 422884, "stop": 423831, "strand": "-", "frame": 1, "gene": null, "product": "MORN repeat protein", "db_xrefs": [ "RefSeq:WP_013526824.1", "SO:0001217", "UniParc:UPI00000C02A4", "UniRef:UniRef100_C4XF99", "UniRef:UniRef50_C4XF99", "UniRef:UniRef90_C4XF99" ], "nt": "ATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCGAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGTATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MQVFNALENEEIINRRNYEKNGTNFELISTKSGLTLAHNVLNDNSQEYYEMNEGAFSGRYYKIAENNQLVGVANYQNDELNGEATIYNTKGDILYKAHFEDGLLNGAVEEYKDGALVKSTMYENGFKTGEEVVYDAKGEVEGKTNFITNNEVKEKEVKEYKEADKEKKAEIRNKNFKEVLKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "0cdba05f663e8efc85d8182828c25163", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A4", "ncbi_nrp_id": "WP_013526824.1", "uniref100_id": "UniRef100_C4XF99", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A4", "RefSeq:WP_013526824.1", "UniRef:UniRef100_C4XF99" ] }, "ips": { "uniref100_id": "UniRef100_C4XF99", "uniref90_id": "UniRef90_C4XF99" }, "psc": { "uniref90_id": "UniRef90_C4XF99", "product": "MORN repeat protein", "uniref50_id": "UniRef50_C4XF99" }, "pscc": { "uniref50_id": "UniRef50_C4XF99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF99" ], "product": "MORN repeat protein" }, "genes": [], "id": "EFPKPENHBP_786", "locus": "GCA000186005_00758" }, { "type": "cds", "sequence": "contig_1", "start": 423833, "stop": 424021, "strand": "-", "frame": 3, "gene": null, "product": "ICEF-IA ORF6", "db_xrefs": [ "RefSeq:WP_013526825.1", "SO:0001217", "UniParc:UPI00000C073C", "UniRef:UniRef100_Q8G8G5", "UniRef:UniRef50_Q8G8G5", "UniRef:UniRef90_Q8G8G5" ], "nt": "ATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAA", "aa": "MKRYFYRIRVKKNLESKKTKSYAVCSNFNEADKIANEIIEKGDYGWVQVERVEKIKHYFKEV", "aa_hexdigest": "536d7d5d695db8bc2e435e625cfadd0d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C073C", "ncbi_nrp_id": "WP_013526825.1", "uniref100_id": "UniRef100_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C073C", "RefSeq:WP_013526825.1", "UniRef:UniRef100_Q8G8G5" ] }, "ips": { "uniref100_id": "UniRef100_Q8G8G5", "uniref90_id": "UniRef90_Q8G8G5" }, "psc": { "uniref90_id": "UniRef90_Q8G8G5", "product": "ICEF-IA ORF6", "uniref50_id": "UniRef50_Q8G8G5" }, "pscc": { "uniref50_id": "UniRef50_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G8G5" ], "product": "ICEF-IA ORF6" }, "genes": [], "id": "EFPKPENHBP_787", "locus": "GCA000186005_00759" }, { "type": "cds", "sequence": "contig_1", "start": 424164, "stop": 424391, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGA", "aa": "MPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALA", "aa_hexdigest": "a7bce82dcd65675848f6e9422130e73d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8724.933399999994, "isoelectric_point": 9.221702766418456 }, "id": "EFPKPENHBP_788", "locus": "GCA000186005_00760" }, { "type": "cds", "sequence": "contig_1", "start": 424404, "stop": 424988, "strand": "-", "frame": 2, "gene": "traD", "product": "TraM recognition site of TraD and TraG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1", "UniRef:UniRef90_A0AB32XBH1" ], "nt": "ATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAACTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGA", "aa": "MLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNES", "aa_hexdigest": "a58b5514450262ccad7d519232569fc7", "start_type": "ATG", "rbs_motif": null, "genes": [ "traD" ], "truncated": "both", "pseudogene": { "start": 424101, "stop": 425270, "observations": { "mutation": [ 424166, 424406, 425225 ], "stop": [ 424166, 424406, 425225 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBH1", "query_cov": 1.0, "subject_cov": 0.470873786407767, "identity": 1.0, "score": 343.0, "evalue": 1.9e-115, "gene_start": 1, "gene_end": 194, "reference_start": 95, "reference_end": 288, "reference_sequence": "MSICYINAETNKRYYWAELFKNVKTIANIVPETLTGNLSHTTSALKNFTEDEFIKRLTRETKSFDLHGIVSSNEPYVIFIHYPDHKPSNHFLVSMLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNESWNYQDLPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALAWQKILSFQQKYKNKLSSELVNDFTKSINICLDQMNDIKTAGAKT" }, "paralog": false, "identity": 0.9897172236503856, "score": 728.0, "evalue": 1.37e-269, "description": "Internal stop codon at 424166, 424406, 425225. Nonsense mutation around 424166, 424406, 425225.", "psc": { "uniref90_id": "UniRef90_A0AB32XBH1", "product": "TraM recognition site of TraD and TraG", "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBH1", "UniRef:UniRef50_A0AB32XBH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1" ] } }, "id": "EFPKPENHBP_789", "locus": "GCA000186005_00761" }, { "type": "cds", "sequence": "contig_1", "start": 425289, "stop": 425429, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTAA", "aa": "MWVDNAKLIVNVVAQFLLLLSIDRPDLLPERHFNLQSVASMCNLDT", "aa_hexdigest": "1994a274baad84226fc2088274757087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5254.1116, "isoelectric_point": 4.74869327545166 }, "id": "EFPKPENHBP_790", "locus": "GCA000186005_00762" }, { "type": "cds", "sequence": "contig_1", "start": 425514, "stop": 425816, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTGTTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGA", "aa": "MNEPESDINSVVLGGVGSGKTQRVLLPNIIYNSHLRYKYRANFCVPDPKKEIIKMVGKKLEKQGYKIYAVDFSDTKKSVGWNPLSFAYKLAHRVPEGHPK", "aa_hexdigest": "226e327f60fcb50bfcc7eed04607c657", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11335.019999999991, "isoelectric_point": 9.68065319061279 }, "id": "EFPKPENHBP_791", "locus": "GCA000186005_00763" }, { "type": "cds", "sequence": "contig_1", "start": 425955, "stop": 426251, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTCCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGA", "aa": "MGDKKKKILKISLSAVAILLAAIIIFLALAIIFNVKRLKSFKMVSIKDFFAQTKASIYAFPKQFLISYAFLLIASIAGISYLFYKNKLKTSLLKPNKY", "aa_hexdigest": "a7ee8fb1bcb6146dfc81f181ec136612", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11069.498099999988, "isoelectric_point": 10.299291801452636 }, "id": "EFPKPENHBP_792", "locus": "GCA000186005_00764" }, { "type": "cds", "sequence": "contig_1", "start": 426251, "stop": 426472, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAA", "aa": "MVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ", "aa_hexdigest": "4e375f5225f369e001680770108e444f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 426251, "stop": 426721, "observations": { "mutation": [ 426508, 426667 ], "stop": [ 426508, 426667 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.46794871794871795, "identity": 1.0, "score": 147.0, "evalue": 3.96e-44, "gene_start": 1, "gene_end": 73, "reference_start": 84, "reference_end": 156, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9807692307692307, "score": 295.0, "evalue": 1.61e-104, "description": "Internal stop codon at 426508, 426667. Nonsense mutation around 426508, 426667.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8364.290099999997, "isoelectric_point": 8.664953804016115 }, "id": "EFPKPENHBP_793", "locus": "GCA000186005_00765" }, { "type": "cds", "sequence": "contig_1", "start": 426812, "stop": 427153, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATAGGTTCAATAAGTCTTATAAACTCGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGCGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENRFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ed4157fbcdf2d18665df0a32937d7513", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13380.135499999978, "isoelectric_point": 9.546301078796386 }, "id": "EFPKPENHBP_794", "locus": "GCA000186005_00766" }, { "type": "cds", "sequence": "contig_1", "start": 427259, "stop": 427399, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "EFPKPENHBP_795", "locus": "GCA000186005_00767" }, { "type": "cds", "sequence": "contig_1", "start": 427517, "stop": 427690, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGTTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTGATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGDLGLEHNVLTAND", "aa_hexdigest": "a6e4eac163e8f8773d728fed3a44313e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6295.746999999999, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_796", "locus": "GCA000186005_00768" }, { "type": "cds", "sequence": "contig_1", "start": 428451, "stop": 428921, "strand": "-", "frame": 2, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGCTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTGATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGDKDWKIFLLFGLANL", "aa_hexdigest": "64ceadfbbde65f76d363fcfbc070e5a5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 428202, "stop": 428993, "observations": { "mutation": [ 428432, 428453 ], "stop": [ 428432, 428453 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 0.9940000000000001, "score": 300.0, "evalue": 2.18e-101, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9695817490494296, "score": 496.0, "evalue": 4.29e-181, "description": "Internal stop codon at 428432, 428453. Nonsense mutation around 428432, 428453.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "EFPKPENHBP_797", "locus": "GCA000186005_00769" }, { "type": "cds", "sequence": "contig_1", "start": 429206, "stop": 429979, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "SO:0001217", "UniRef:UniRef50_Q8GCP5", "UniRef:UniRef90_Q8GCP5" ], "nt": "ATGTTACATATGGAGATATTGCAAGAGAGATTAAATCGTGAATATAAAATCGGTATTATAGCAACTAGTCCTTCAGTTGAATATGATGTTCATATGACAGATGGCAGCATTAAAAAAATATCAAACCCTGCACTTTTACCTGATCGAACTTTTATCGATCATATTGATGAACCTTACATTAAAGCTGAGATTTTGGTACCTAATGAATACATAGGAAATGTTATGGAACTATGTCAAAATAAACGTGGAATTTATATCAATATGGACTATATTGATGCTAATAGATCAAGGCTTATTTATGAAATGCCACTAGGCGAAATTATCTTTGATTTCTTTGATAGACTTAAGAGTCTAACTAAAGGTTATGCATCATTTGAATATGACCTAATAGGCTATAAAACAAGTGATTTGGTAAAAGTAGACATACTTTTAAATGGTGATAAAATTGATGCTTTTTCAATTATTACTCATAAAGATTCAGCTTATAGTAAAGCTCGTGATTTAACTCAAAAATTAAAAGAAGCTATACCAAGACAAAACTTTGAAGTACCTGTTCAAGCAACTATTGGAAGTAAAATTATTGCTAGAGAAACTATTAAAGCTTATCGTAAAGATGTTACTTCAAAACTTCATGCAGCTGATGTTTCAAGATATAAAAAACTTTTAGAAAAGCAAAAAGCGGGTAAGAAAAAAATGAAAATGCTTGGTACTGTTGAAGTTCCTCAAGAAGCATTTTTATCAATTCTTAAAACTAATATTGATAAGAAAAAATAG", "aa": "MLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK", "aa_hexdigest": "fc4c3be7f24ef9cedbc56b387e762b6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8741496598639455, "identity": 0.767, "score": 399.0, "evalue": 4.02e-138, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 429206, "stop": 430279, "observations": { "mutation": [ 430036 ], "stop": [ 430036 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8GCP5", "query_cov": 1.0, "subject_cov": 0.4290484140233723, "identity": 1.0, "score": 501.0, "evalue": 7.02e-174, "gene_start": 1, "gene_end": 257, "reference_start": 343, "reference_end": 599, "reference_sequence": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSITWEQETSKALGFGFRVGFLGMLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK" }, "paralog": false, "identity": 0.9971988795518207, "score": 695.0, "evalue": 9.37e-253, "description": "Internal stop codon at 430036. Nonsense mutation around 430036.", "psc": { "uniref90_id": "UniRef90_Q8GCP5", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q8GCP5", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8GCP5", "EC:3.6.5.n1", "UniRef:UniRef50_Q8GCP5", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "pscc": { "uniref50_id": "UniRef50_Q8GCP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8GCP5" ], "product": "Elongation factor 4" } }, "id": "EFPKPENHBP_798", "locus": "GCA000186005_00770" }, { "type": "cds", "sequence": "contig_1", "start": 430034, "stop": 431005, "strand": "-", "frame": 3, "gene": null, "product": "Elongation factor 4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "nt": "ATGGACAAAAGCAAAATTAGAAATTTTTCAATTATTGCTCACATAGATCATGGTAAAAGTACGCTTGCTGATAGAATTCTAGAAATTACTAATACAGTTAGTGACCATGATATGGATGATCAATTTTTGGACCAAATGGATTTAGAACGTGAAAGAGGAATCACAATTAAATTAAATGCTGTACAAATTAAGTATAAAGATTACGTATTCCATTTAATTGATACACCAGGTCACGTTGATTTTACTTATGAAGTTTCACGTTCATTAGCTGCTAGTGAAGGAGCATTATTATTAGTTGATGCTACTCAAGGAATTGAAGCTCAAACTTTAGCTAATGTTTATTTAGCTCTTGAAAATAATTTAACTATTATTCCTATTATTAATAAAATTGATTTACCTTCAGCTGATATTGAAGCCACAAAAAATGAAATAGAAGAAGTGATTGGTCTACCAACTGATAATGCAGTTTGCATTTCAGCTAAAACTGGTTTAAATTGTGAAGCAGTTTTAGCTGCAATTGAAAAATATATTCCAGCACCTAAAGATGCTGATGATTCTAAACCTCTTAAAGCTTTAATTTTTGACTCGTACTTCGATGAATATCGTGGTGTAGTTATGCTTATAAGAATTTTTGAAGGTGAATTAAAAGTTGGCGATGAATTTATGTTTATGTCGAATGGACAAAGATATTTTGTTAGTGAACTTGGAGTTAGAAGTCCAAAAGAAACTAAGAAAAAGAGACTTGAAGCAGGTGAAGTTGGATATGTAGCAGCTACTATTAGAGATGCTAGAGAAGTAAGCGTTGGAGATACTATAACTTTAGTTAATAGACCAGCTGAAAAAGCACTGCCAGGTTACAAAAAGAAAAAACCTGTGCTTTATACTGGGTTTTATCCAATTGATACTCGCGACTATATGGAACTTAAAGAAAGTTTAGAAAAAATAGCTCTTTCAGATAGCTCAATAACTTGA", "aa": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSIT", "aa_hexdigest": "3da9e17740625522f70716f9a6309cba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BB04", "query_cov": 0.9256965944272446, "subject_cov": 0.9373040752351097, "identity": 0.833, "score": 494.0, "evalue": 1.66e-174, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A448ZZP8" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "product": "Elongation factor 4" }, "genes": [], "id": "EFPKPENHBP_799", "locus": "GCA000186005_00771" }, { "type": "cds", "sequence": "contig_1", "start": 431093, "stop": 431263, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTTTAATTAAAAAAGGACATACTAACATTTCATATCGAGATGAAAATAAGTTTATTCAAGAAAAAATTTTAAATAAATTTAATCACAAATTAGAATACAGTTTATTAAAAGATTTTAGTTTTACACCAAAACTTATTAGCCAAAATGACAAAGAAATCACTTGA", "aa": "MKVLIKKGHTNISYRDENKFIQEKILNKFNHKLEYSLLKDFSFTPKLISQNDKEIT", "aa_hexdigest": "96cc984c8c4b9940453da2d23516638b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6727.7394, "isoelectric_point": 9.522963523864746 }, "id": "EFPKPENHBP_800", "locus": "GCA000186005_00772" }, { "type": "cds", "sequence": "contig_1", "start": 431978, "stop": 432082, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAAGGCAAAAAAATTAAATACGCTTCTGTCATTACTTTGTCAATTATTTTAGCAATATTTACTGTTTTATTAATTATTGTTGCTAAAGGAAGTTGA", "aa": "MSDKGKKIKYASVITLSIILAIFTVLLIIVAKGS", "aa_hexdigest": "fadfcd6737dfef9ae20a803efaf1bb28", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3635.4870999999994, "isoelectric_point": 10.000287437438963 }, "id": "EFPKPENHBP_801", "locus": "GCA000186005_00773" }, { "type": "cds", "sequence": "contig_1", "start": 432083, "stop": 432280, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGTAAGTGAAAAAAGTGGAGATGCAGGTAAGTTTTTAATAGCAAACAGACTTGTAACAAATGGAATTTTTGCAGACTTTTCAAACTTCACAAGTAATTTTTCAATAATTAAAAGCGTAGTTGAATCAGACACAATGGTTTATATAGCTTGTGGAATCTCAGCATTGTATTTTATCATTTATATTTCATGA", "aa": "MAKVSEKSGDAGKFLIANRLVTNGIFADFSNFTSNFSIIKSVVESDTMVYIACGISALYFIIYIS", "aa_hexdigest": "b777bb95341859c8d374dde6e0ccc2be", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.114299999999, "isoelectric_point": 5.997616386413574 }, "id": "EFPKPENHBP_802", "locus": "GCA000186005_00774" }, { "type": "cds", "sequence": "contig_1", "start": 432413, "stop": 432553, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGAAAGCGATGAAAAATTCATCGGTGGAGTAATACCATTTATCCAATTCTTTACACACATTGGTGTTGTGGTAGTTTCTATTGTTGGTATTATTATTGAAAGCGTTAAAGGTAAAGAAAAGAAAAATGTTTGA", "aa": "MTKESDEKFIGGVIPFIQFFTHIGVVVVSIVGIIIESVKGKEKKNV", "aa_hexdigest": "ece4e6e2520054c07d59d020cc718c29", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.974999999999, "isoelectric_point": 8.121743202209473 }, "id": "EFPKPENHBP_803", "locus": "GCA000186005_00775" }, { "type": "cds", "sequence": "contig_1", "start": 432761, "stop": 433138, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACAATGAAGAAAAACAATATCAAAGTATTCTAGACAATTTACTAGATGTTAGTCCAAATGACAGTGCAGTATTTACTAGAGTAAATAACAAATTTGCTTTTGATTTTTATACTATTTTTGGACCTGAAAGTTTTAGCAAAATTTACAACAATAAAAACTTTGACATACCTATTCTTGAAGTTAATTTGATCAGAATTTGTAATTTACTTGAAGAAGCAAATACATATGAAGAAATTATTAAAATTCTTGAAGAAAACAATATTGAAGTAAGTGAAAAACGTAATAGTCAATTAAAGAAAAATCCTCTTGGAATTAAAAATAACATTATTAATGAATTAAAAGTTAATTTTCAAAAACAATCATTAAAATGA", "aa": "MSNNEEKQYQSILDNLLDVSPNDSAVFTRVNNKFAFDFYTIFGPESFSKIYNNKNFDIPILEVNLIRICNLLEEANTYEEIIKILEENNIEVSEKRNSQLKKNPLGIKNNIINELKVNFQKQSLK", "aa_hexdigest": "ce8210b51537f9379f31bdd9e1b7bf39", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14605.367199999982, "isoelectric_point": 5.0644338607788075 }, "id": "EFPKPENHBP_804", "locus": "GCA000186005_00776" }, { "type": "cds", "sequence": "contig_1", "start": 433193, "stop": 433399, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTAGGTTTCTTATTTGTTAAATTAGTTATTGACGATAAAGCTGTTTATGGTCCTTTATTTTTAAAAGAAGTTTATTTAGAAATTAGAAATGCTAGACCATATTTAGTTTCAAATGGTGACATTAAAATTAATGAAAAATTGCTATTCTTATTAAGTAATGCAGGTTTTGATTTAAATATCTCGGAAAACTATGGTGATTGA", "aa": "MHLGFLFVKLVIDDKAVYGPLFLKEVYLEIRNARPYLVSNGDIKINEKLLFLLSNAGFDLNISENYGD", "aa_hexdigest": "267726fef962aee9d0c9b16e4333d5e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7777.940599999999, "isoelectric_point": 5.184250450134276 }, "id": "EFPKPENHBP_805", "locus": "GCA000186005_00777" }, { "type": "cds", "sequence": "contig_1", "start": 433517, "stop": 433771, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGAACCAGGCGTAGTTTTAGGCTTATTCCAACCAAGTGGTGGCTATGCCAGAAATAGAATGCTTGAAATAATTAAGAATAAAGAATTGAACAAAATTTTAGATATAGAATTTAACAAAAACATCTATAAAAATAGGGTAAAACAAACAATTTACAACCCTAAAACATCTATTTTTAAAATTACACCTACCAACTATTCTCAAGATAAAGCTATAGCTTCAAGCTTAAATCAAAACACAATTATTTGA", "aa": "MEFEPGVVLGLFQPSGGYARNRMLEIIKNKELNKILDIEFNKNIYKNRVKQTIYNPKTSIFKITPTNYSQDKAIASSLNQNTII", "aa_hexdigest": "161ac7f1ea6832377a31b4414712c963", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9665.068599999991, "isoelectric_point": 9.646098136901855 }, "id": "EFPKPENHBP_806", "locus": "GCA000186005_00778" }, { "type": "cds", "sequence": "contig_1", "start": 433814, "stop": 434140, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAACCTTTTAGTTTATGGCAAAAATTCTTTAGTTTGTTCTCAAAAGAAAGCGGCTCTTGAAGTTATTAAAAATAGAATGGGCGCTTTAAAAATCTTTTGTCTTTTCATGCTTAATTCAAAAAACATGAACAAAAAGAATTTCTACTTGCCTCTTAGAGAATATTTAGATTATTTAGAAAACTATGATGATAATCCTAATTTAAAACCATTAAGAATCATTAACCAAAAAGATATTCGTTTTGTTAACAATATAGCTAATTTCAGTAGTGATCCACGTTTTGTTAATATCACTCAAGCTTTAGCTAAAGTTCATAATAATTGA", "aa": "MTNLLVYGKNSLVCSQKKAALEVIKNRMGALKIFCLFMLNSKNMNKKNFYLPLREYLDYLENYDDNPNLKPLRIINQKDIRFVNNIANFSSDPRFVNITQALAKVHNN", "aa_hexdigest": "04326da75199e2c7d39d167e4568af7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12600.588499999976, "isoelectric_point": 9.696447944641115 }, "id": "EFPKPENHBP_807", "locus": "GCA000186005_00779" }, { "type": "cds", "sequence": "contig_1", "start": 434321, "stop": 434671, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAACACATTTAAAGAATTTCAAGGAAATTTAAATGATTCAGTTCAAGTTTTAAAAAACTTAAAGCAAGAAGACTTTGAATTTTTAAATGATTTAATAAACATTTTGCCTTCTAATAATACTCAAGAAACTAGCGATGAAAATGAATTAAAGAAATTTATTGCTCAAGGTATTATTAAACGAATTAACAAATTTACTGATGAAGAAAAAGATCAATACTCAGAATTTGCAGCCACAATTAGATTAGGAACATTAGAACCTTACAAGTTTATTAAGCGTTTTCCTAATATGATTAAAAAGATTTTCCCTATTGTAATTGTGACACCTGAAGCTGACTTAAGTGCCTGA", "aa": "MFNTFKEFQGNLNDSVQVLKNLKQEDFEFLNDLINILPSNNTQETSDENELKKFIAQGIIKRINKFTDEEKDQYSEFAATIRLGTLEPYKFIKRFPNMIKKIFPIVIVTPEADLSA", "aa_hexdigest": "0e7b14cd6ddc9d0f5dd14f3f8bc4719b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13523.284199999978, "isoelectric_point": 5.02566967010498 }, "id": "EFPKPENHBP_808", "locus": "GCA000186005_00780" }, { "type": "cds", "sequence": "contig_1", "start": 434894, "stop": 435046, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAGACAAAAACTATCGTTCAAATTATGCTTCACTGATGACTTTTAGCTCTAAATACTTCTACAAAAGTACTTTGGATGTTATCGATTCAGCTAATATGGAAGCTAATTTCAAGCCAATTGAAGTACATGAAGTTAATGGCCTTTGA", "aa": "MLLDKNYRSNYASLMTFSSKYFYKSTLDVIDSANMEANFKPIEVHEVNGL", "aa_hexdigest": "f812a67849f10f5f88119ebd0c2cab77", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5796.5169, "isoelectric_point": 5.564048576354979 }, "id": "EFPKPENHBP_809", "locus": "GCA000186005_00781" }, { "type": "cds", "sequence": "contig_1", "start": 435128, "stop": 435460, "strand": "+", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "nt": "TTGTTGTGCTTCAATGCTAAACAACAAGATTCAATTACCACAAGCATTTTCAAAAATCATCCTGAATTAGAAAAAGCAATTAATAATGGAAACTTGTTATTAAGAAACATTGAAAATATTCAAGGCGATGAAGCTGATTTAGTTATAGCTTCAGTAGCTTATGATAAAGATACAGCAATTCACTCAACTTATGTTGGACGTCCTGGCGGTATGAATGCGTTAAATGTGGCAGTTAGTCGTGCTAAGGATAAAATGATTGTTATTAAATCACTTAAATCTAGTGACTTAACTATAATGACAGGTAATGAAGATGCTATTATGTTCAAACATTGA", "aa": "MLCFNAKQQDSITTSIFKNHPELEKAINNGNLLLRNIENIQGDEADLVIASVAYDKDTAIHSTYVGRPGGMNALNVAVSRAKDKMIVIKSLKSSDLTIMTGNEDAIMFKH", "aa_hexdigest": "8fbb0e89d4f835c318bccc7213e428da", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00313CCABD", "query_cov": 0.990909090909091, "subject_cov": 0.8384615384615385, "identity": 0.55, "score": 114.0, "evalue": 1.37e-30, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI00313CCABD" }, "pscc": { "uniref50_id": "UniRef50_UPI00313CCABD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_810", "locus": "GCA000186005_00782" }, { "type": "cds", "sequence": "contig_1", "start": 435539, "stop": 435838, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCATTGAAACTGAACCAGTTGAAGTTGAGAATTCATTATTAAATCAAGAAATCAAAAGCACAATTCTTGAAGCAATTAAAGATAAACCTTATCTAAAATTAATTCCAAATGAAACACTTGGAACACTTAAAGTTGATTATGTTTTAAGAAGAAAACATGAACCAATAATGACATTTATGATCGATGATTACAAATATGTTAATAATCCTGAAGACTTTGTTATTTTTAAAGACTTGTGTAAATTTATTAGATCTAAAAAATATATTGTTTATAAACTAGATCGAATTTTATGA", "aa": "MQTIETEPVEVENSLLNQEIKSTILEAIKDKPYLKLIPNETLGTLKVDYVLRRKHEPIMTFMIDDYKYVNNPEDFVIFKDLCKFIRSKKYIVYKLDRIL", "aa_hexdigest": "4c678a3d073495c3420da6f6874b85dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11841.784199999986, "isoelectric_point": 6.582262229919434 }, "id": "EFPKPENHBP_811", "locus": "GCA000186005_00783" }, { "type": "cds", "sequence": "contig_1", "start": 435893, "stop": 436570, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCAAGTAGTTGAAGTAACTAAAGAAATACCAACAACTCAATTAGAAATTCTTGAAGCAACAAACCAAGAAATTAAAGAAGCAAATGAAGTTATTGAAGAAGAAAATCAAGTTAAAGCAATAATAATTAATGAAAATAGTCAAGTAATTAACGTTAATGAAGAAGACTTTATCAGTCAAGAACACTCAGATACTATTATAGTTGAAGAAATTAAAGAAGTACCAGTTTTAGATAATGAAAAGAAAGATGAAACTATTGAAGAAGTTTTAGAAAAAATTAGCCAAGATCGTGAAAACACCAAAACTCAAGAAATTGAAAAATCTAAAGAAAATACAAAAGTTGTTGAAGCTATCAAAAAAGAAGAAACAGCTCAAGACACAATTACTTTGAATGCAGAAAAAACTCAAGAAGTTGGTTTTATTAAAGATGAAGCAACTATGGTTATTGAGCTTAATGTTGAAGAACAAAATGAACAAATTCAAAGTGTTTCACATTCGACTGAAAACACTATAAAACAGGGAGAAACAAAAAATATTGAAGCTGAAAATGCAATTGAAGAACAAGAAAAAACACTTGATCAATTAATCGAAGAAGGCGATAAAATTAAACAAAGTGATTATCTTGAAAGAGAAGAAGTTCCAGTTAAAGATTTTGAAGAATCAACAAAGGAATGA", "aa": "MTQVVEVTKEIPTTQLEILEATNQEIKEANEVIEEENQVKAIIINENSQVINVNEEDFISQEHSDTIIVEEIKEVPVLDNEKKDETIEEVLEKISQDRENTKTQEIEKSKENTKVVEAIKKEETAQDTITLNAEKTQEVGFIKDEATMVIELNVEEQNEQIQSVSHSTENTIKQGETKNIEAENAIEEQEKTLDQLIEEGDKIKQSDYLEREEVPVKDFEESTKE", "aa_hexdigest": "7a300d8eea78da7a586e90152292cf34", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25872.054900000025, "isoelectric_point": 4.257149696350097 }, "id": "EFPKPENHBP_812", "locus": "GCA000186005_00784" }, { "type": "cds", "sequence": "contig_1", "start": 436835, "stop": 437263, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAACAACTATTAAATGCATCTCTAATTTTGATGAAATCCAAAAGTATATAGGATTGTTGTTTTTCAATGTTTTAATAATAAAACTTCAATTTTTTTCTAATTTATCAATGTTTTTAATAAATTCTTTATATTATCCAACTCTAATACCAGTTGAAATAAACACACGAAAAATGATACTAAATTTAGTGTTTTTGTGGCTTTTTAATTCTTCAACAAGCTTAATGAATTCCTTTTCAAGTATAGCTTGTCTTTTGTCTTTTTCTTCTTTTATATATAGAAGTTCATTTTTAACATCTGTATTTGTATTAGTGATTTTGCAAAATCAATTTAAAAAAGAAGAAAATATGTTTCTTTTAGTTAATTTAGTATTATTTGAAATTTTATTATTTGCTAATTCACGATTCATCCTTATAATTAATTGA", "aa": "MKLTTIKCISNFDEIQKYIGLLFFNVLIIKLQFFSNLSMFLINSLYYPTLIPVEINTRKMILNLVFLWLFNSSTSLMNSFSSIACLLSFSSFIYRSSFLTSVFVLVILQNQFKKEENMFLLVNLVLFEILLFANSRFILIIN", "aa_hexdigest": "19400a15aad0ab8224e517766e270f2b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16629.7832, "isoelectric_point": 9.263607215881347 }, "id": "EFPKPENHBP_813", "locus": "GCA000186005_00785" }, { "type": "cds", "sequence": "contig_1", "start": 437505, "stop": 437636, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "EFPKPENHBP_814", "locus": "GCA000186005_00786" }, { "type": "cds", "sequence": "contig_1", "start": 437766, "stop": 438230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTADEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "d58bf7ad8ae3df16d904eca596400295", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 437505, "stop": 438266, "observations": { "mutation": [ 437634, 437712, 437736, 437751, 438228 ], "stop": [ 437634, 437712, 437736, 437751, 438228 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.935, "score": 271.0, "evalue": 2.78e-90, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8932806324110671, "score": 432.0, "evalue": 5.48e-156, "description": "Internal stop codon at 437634, 437712, 437736, 437751, 438228. Nonsense mutation around 437634, 437712, 437736, 437751, 438228.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17768.86899999999, "isoelectric_point": 5.962433052062988 }, "id": "EFPKPENHBP_815", "locus": "GCA000186005_00787" }, { "type": "cds", "sequence": "contig_1", "start": 438285, "stop": 438917, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATTGAAACAACAGTTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSSKSLTSIINGLNEFNSFVESSNQISFEKLDEFINKTQAILNDENNLTTSIQSVIKEFLNAYNSNVDSIETTVRLAKLNNSKQIIDEFVQKSKIELNSLK", "aa_hexdigest": "beb44c1c06e9e97a51f7aebf68fc9063", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 438285, "stop": 439115, "observations": { "mutation": [ 438915, 438996 ], "stop": [ 438915, 438996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9570000000000001, "score": 369.0, "evalue": 2.45e-127, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9420289855072463, "score": 494.0, "evalue": 3.12e-179, "description": "Internal stop codon at 438915, 438996. Nonsense mutation around 438915, 438996.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23710.505700000023, "isoelectric_point": 5.4200754165649405 }, "id": "EFPKPENHBP_816", "locus": "GCA000186005_00788" }, { "type": "cds", "sequence": "contig_1", "start": 439647, "stop": 439814, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "1160043971d8aa939f463149be73f6fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6202.908700000004, "isoelectric_point": 7.942263221740723 }, "id": "EFPKPENHBP_817", "locus": "GCA000186005_00789" }, { "type": "cds", "sequence": "contig_1", "start": 439926, "stop": 440144, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTACAAACACTAATTTAATTAAAAATTATTTAGAAAATGATTATTTATTAGCTGTTAAAGATTTAAGAGAATATTTCATAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNTNLIKNYLENDYLLAVKDLREYFINKLIYLLHEKFNYGDVY", "aa_hexdigest": "15caef378a4fc0f00bb59b16fd4d993f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.452699999998, "isoelectric_point": 4.867032051086425 }, "id": "EFPKPENHBP_818", "locus": "GCA000186005_00790" }, { "type": "cds", "sequence": "contig_1", "start": 440643, "stop": 440807, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAATGTCAGATTCTAAAATAATGTTTAAAGATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKMSDSKIMFKDDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "18bfa3abdb1bd2c335e94e6ecba71249", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6356.1726, "isoelectric_point": 7.940844917297363 }, "id": "EFPKPENHBP_819", "locus": "GCA000186005_00791" }, { "type": "cds", "sequence": "contig_1", "start": 440948, "stop": 441073, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCTGAATTATGTTCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCACGTTTTGTTATATAG", "aa": "MKAELCSTVLKYENNVLENGIYSNSGLVLSYFSSDFPRFVI", "aa_hexdigest": "beb5d1d27752508245a5a3598068f700", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4649.2559, "isoelectric_point": 4.867770957946777 }, "id": "EFPKPENHBP_820", "locus": "GCA000186005_00792" }, { "type": "cds", "sequence": "contig_1", "start": 441607, "stop": 441780, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354617.1", "SO:0001217", "UniParc:UPI0001E330E8", "UniRef:UniRef100_A0AB32XBI3", "UniRef:UniRef50_C4XFB5", "UniRef:UniRef90_C4XFB5" ], "nt": "TTGTTAAAATTAAGATATGAAAAATTTAAAGTCAAAGTTACTTCAAAAAAATATTTAAAATTTTTACCTGAAAATGAAACGTATAGATTCATAGTCGATTATATCGACAATAAAACAGAAATTTTTTTAAACAACTCTTCAATTGAATTAACTGAAAAAATGTTTGTTTCATAG", "aa": "MLKLRYEKFKVKVTSKKYLKFLPENETYRFIVDYIDNKTEIFLNNSSIELTEKMFVS", "aa_hexdigest": "3a10f14dffe7222b9b1a7448407b93a7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330E8", "ncbi_nrp_id": "WP_013354617.1", "uniref100_id": "UniRef100_A0AB32XBI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330E8", "RefSeq:WP_013354617.1", "UniRef:UniRef100_A0AB32XBI3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBI3", "uniref90_id": "UniRef90_C4XFB5" }, "psc": { "uniref90_id": "UniRef90_C4XFB5", "uniref50_id": "UniRef50_C4XFB5" }, "pscc": { "uniref50_id": "UniRef50_C4XFB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6974.100399999999, "isoelectric_point": 9.260125923156735 }, "id": "EFPKPENHBP_821", "locus": "GCA000186005_00793" }, { "type": "cds", "sequence": "contig_1", "start": 441907, "stop": 442155, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526834.1", "SO:0001217", "UniParc:UPI0001F5A770", "UniRef:UniRef100_C4XFB6", "UniRef:UniRef50_C4XFB6", "UniRef:UniRef90_C4XFB6" ], "nt": "ATGCATGAAATAAGTAAAAACATCTTTGCTTACTATACTTCAAGCTTACAAAAAACTTATGAGTCAATTAACAAAGAAGAAATTAAAGAGTATTGCAATTTATTAAGCGAAACTAAAAATCACATATTTTTTGGTATTGGGCAAAGTGAAAAAGTAGCTAGTTATTTACGTGAAAATTTAAACAAAATTAGACTTACTTCATTACCTATTAACAATATGCATGATTTTTTTAACATAGTTTATGTTTAA", "aa": "MHEISKNIFAYYTSSLQKTYESINKEEIKEYCNLLSETKNHIFFGIGQSEKVASYLRENLNKIRLTSLPINNMHDFFNIVYV", "aa_hexdigest": "6cb27aec406fca34ebacc45794ec036a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A770", "ncbi_nrp_id": "WP_013526834.1", "uniref100_id": "UniRef100_C4XFB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A770", "RefSeq:WP_013526834.1", "UniRef:UniRef100_C4XFB6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFB6", "uniref90_id": "UniRef90_C4XFB6" }, "psc": { "uniref90_id": "UniRef90_C4XFB6", "uniref50_id": "UniRef50_C4XFB6" }, "pscc": { "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB6" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 441787, "stop": 442155, "observations": { "mutation": [ 441874 ], "stop": [ 441874 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB6", "query_cov": 1.0, "subject_cov": 0.6721311475409836, "identity": 1.0, "score": 166.0, "evalue": 9.8e-52, "gene_start": 1, "gene_end": 82, "reference_start": 41, "reference_end": 122, "reference_sequence": "MTRFARAASFISFKSLQIYITKRTIVTDDWTRINVGSNLSMHEISKNIFAYYTSSLQKTYESINKEEIKEYCNLLSETKNHIFFGIGQSEKVASYLRENLNKIRLTSLPINNMHDFFNIVYV" }, "paralog": false, "identity": 0.9836065573770492, "score": 238.0, "evalue": 2.19e-82, "description": "Internal stop codon at 441874. Nonsense mutation around 441874.", "psc": { "uniref90_id": "UniRef90_C4XFB6", "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB6", "UniRef:UniRef50_C4XFB6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9712.957199999992, "isoelectric_point": 6.821440696716309 }, "id": "EFPKPENHBP_822", "locus": "GCA000186005_00794" }, { "type": "cds", "sequence": "contig_1", "start": 442155, "stop": 442262, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTCCAGATGCTTTTGTTACAGTTATTTCGCATTCTTTTGAAACAGCTGAAGTTATAAAAGTTTGTGAAATTTTGGAAAACAAGAACATTAATTATGCTTTATGA", "aa": "MGPDAFVTVISHSFETAEVIKVCEILENKNINYAL", "aa_hexdigest": "d3df982869dcd29e230ffbbbb843dc32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3896.4423000000006, "isoelectric_point": 4.571128273010253 }, "id": "EFPKPENHBP_823", "locus": "GCA000186005_00795" }, { "type": "cds", "sequence": "contig_1", "start": 442736, "stop": 443362, "strand": "+", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.9", "GO:0004845", "GO:0009116", "RefSeq:WP_013354614.1", "SO:0001217", "UniParc:UPI0001A8C2FF", "UniRef:UniRef100_C4XFB8", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_C4XFB8" ], "nt": "ATGTTAAAAGTTTTAGATCATCCGCTTATTTCAATTAAGCTGACTAATATGAGAGATAAAGAAGCAAATCACTCTCGTTTTAGAAGAAACTTAAATGAAATCGCATCATTAATGGTGTATGAAGTTTTAAGAAACTATAAAACTAAAGCTAGAAAAATAGTGACACCTTTAAATCAAGAATATGTAGGAGCTACTTTTGATAAAGAAATAGTTATTGTGCCAATTTTAAGAGCTGGGCTTGGAATGTTAGATGGCTTACTTGAATTAATGCCTGAAGCTAGAGTTGGTCACATTGGACTTTACCGCGATGAAGTAACTCATCAAGCTCATGAGTATTTTTACAAAATGCCAGAAGTTAAAAAAGATAGCTACATTTTTGTAGTTGATCCAATGCTCGCTACTGGCGGTAGCGCAGTTGATGCTATTAAAAAATTAAAATCAGATGGCTTTACTAATATTCATTTAGTGTGCTTAGTTGGAGTTAAAGAAGGAGTAGCAGCTGTTGAAAAGGCTTTTGGTAAAGACTTTGAAATTTATTTAGCTGCTCTAGACAAAAAACTCAATAATCAAAAATATATTGAACCTGGTTTAGGCGACGCAGGAGACCGTATTTTTGGAACAAAATAA", "aa": "MLKVLDHPLISIKLTNMRDKEANHSRFRRNLNEIASLMVYEVLRNYKTKARKIVTPLNQEYVGATFDKEIVIVPILRAGLGMLDGLLELMPEARVGHIGLYRDEVTHQAHEYFYKMPEVKKDSYIFVVDPMLATGGSAVDAIKKLKSDGFTNIHLVCLVGVKEGVAAVEKAFGKDFEIYLAALDKKLNNQKYIEPGLGDAGDRIFGTK", "aa_hexdigest": "257b399ec7c1330e5d86530cc756a37d", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C2FF", "ncbi_nrp_id": "WP_013354614.1", "uniref100_id": "UniRef100_C4XFB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2FF", "RefSeq:WP_013354614.1", "UniRef:UniRef100_C4XFB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFB8", "uniref90_id": "UniRef90_C4XFB8" }, "psc": { "uniref90_id": "UniRef90_C4XFB8", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "go_ids": [ "GO:0004845", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "EFPKPENHBP_824", "locus": "GCA000186005_00796" }, { "type": "cds", "sequence": "contig_1", "start": 443349, "stop": 443543, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACAAAATAAAAGTATTTATTATGGCAAAAGTATTGTTAAAAAAATAGTCCAAACTGGAGTTTTATTAGCTTTAGCAATTGTAGTTAATATTACTTGCTCAAGCTTTATGAAATTTCCTTTAGCGCCTTTTTTAAAGTTTGATTTTGCACTTGTTGTCGTTACTTTTAGCGCTTTAGCAATTGCACCTTGA", "aa": "MEQNKSIYYGKSIVKKIVQTGVLLALAIVVNITCSSFMKFPLAPFLKFDFALVVVTFSALAIAP", "aa_hexdigest": "759bf4df9aeac6cfbb6557141f6737f0", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6996.426699999998, "isoelectric_point": 9.581758689880367 }, "id": "EFPKPENHBP_825", "locus": "GCA000186005_00797" }, { "type": "cds", "sequence": "contig_1", "start": 444265, "stop": 444384, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCAAGTGGAAATATTCATATGGGGCATGTTAGAAACTATGCAATCGGCGATGTTTTAGCACGTTATTATCGTCGTAAAGGTTTTAATGTTTTACATCCTTTTGGTTGA", "aa": "MFPYPSGNIHMGHVRNYAIGDVLARYYRRKGFNVLHPFG", "aa_hexdigest": "d46ec1b438548f1f01d589ed7806e043", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.9487179487179487, "hmm_cov": 1.0, "evalue": 7.740411428773164e-08, "score": 27.757923126220703, "start": 3, "stop": 39 } ], "seq_stats": { "molecular_weight": 4552.2083, "isoelectric_point": 10.15604305267334 }, "id": "EFPKPENHBP_826", "locus": "GCA000186005_00798" }, { "type": "cds", "sequence": "contig_1", "start": 444841, "stop": 445302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGGCAAAATTTGACATAGTTGAAAATAATCAAAATCACTTTAGTAATATTGATATTTTTACAAAAAACAAAGATATTTTAGTAACCATGAATTTTATAACTATTGGTGCAAATCATGGCCTTGTTAAACAATTAATTGAAGAAAATAAATTAAGTGATAAAGAAATTGAAAAACTTAATTTAATTAAAGCAAAAGCTACAGCAAAAGATTTTTCGCAAAAATTATGTTTAGCTTTACCACTTCAAGCAATTAATCCAGTAAATAATCAAAAATACAAAATTTATGTTAGTGATTTTTGTTCTCTCGGAGAAAAAAATAGAAGTCAAATTATCGATACTACTAAAGTTAAAAGTCATGAAGAATTTGCCAAATTTAATAACTTAGAAATTACTAATTATGCAAATAACAAAGCTGATTTTAGTAAGCTTGAAAACTCGAGTAAAATCAACTTACAAGATTGA", "aa": "MAKFDIVENNQNHFSNIDIFTKNKDILVTMNFITIGANHGLVKQLIEENKLSDKEIEKLNLIKAKATAKDFSQKLCLALPLQAINPVNNQKYKIYVSDFCSLGEKNRSQIIDTTKVKSHEEFAKFNNLEITNYANNKADFSKLENSSKINLQD", "aa_hexdigest": "3270587a8835bf9ee5d81e0e62c0dc66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 128, "aa_cov": 0.8300653594771242, "hmm_cov": 0.9921875, "evalue": 2.073554742610658e-13, "score": 46.50330352783203, "start": 13, "stop": 139 } ], "seq_stats": { "molecular_weight": 17468.729499999994, "isoelectric_point": 8.439249992370609 }, "id": "EFPKPENHBP_827", "locus": "GCA000186005_00799" }, { "type": "cds", "sequence": "contig_1", "start": 445342, "stop": 445461, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATTGTGCTAAGTGTGGTTTAGTTCCTGAAAAAGAAGAAAACTTACCAGTTACTTTGCCTTACAAAGTCAACTTTACTACTAGTGGCAACCCACTTAAAACAAATAATAAATGA", "aa": "MINCAKCGLVPEKEENLPVTLPYKVNFTTSGNPLKTNNK", "aa_hexdigest": "9efae394d5bc670e937c1677f55fc07d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4306.978400000002, "isoelectric_point": 8.737867546081542 }, "id": "EFPKPENHBP_828", "locus": "GCA000186005_00800" }, { "type": "cds", "sequence": "contig_1", "start": 445660, "stop": 445896, "strand": "+", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "nt": "TTGCACTTACTGTATGCACGTTTCTTTACTAAAGTTTTAGCAGACTTAAAAATGGTTGACTTTAGAGAACCTTTTGATAATTTATTAACTCAAGGAATGATTCTTAAAGATGGACTTAAGATGTCTAAATCAAAAGGCAATGTTGTTAGCCCAGATGAAATGATTAAAAAATATGATGCTGATACTATGAGGCTTTTTGTTCTTTTTGCAGCACCGCCTGAAAAAGAACTTGAATGA", "aa": "MHLLYARFFTKVLADLKMVDFREPFDNLLTQGMILKDGLKMSKSKGNVVSPDEMIKKYDADTMRLFVLFAAPPEKELE", "aa_hexdigest": "24ecdb51c27b920da956198c777c61fb", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002381B5B9", "query_cov": 0.9230769230769231, "subject_cov": 0.8780487804878049, "identity": 0.639, "score": 90.9, "evalue": 1.89e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002381B5B9" }, "pscc": { "uniref50_id": "UniRef50_UPI002381B5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "product": "class I tRNA ligase family protein" }, "genes": [], "id": "EFPKPENHBP_829", "locus": "GCA000186005_00801" }, { "type": "cds", "sequence": "contig_1", "start": 446119, "stop": 446226, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTTTCAATGCTTATGATAAGATTACTAACTCATTATTAATTAAAGAAATGTACTATGTTTTATTAAACATTTTGGAACCATTTATTCCACATTTAGCTTGA", "aa": "METFNAYDKITNSLLIKEMYYVLLNILEPFIPHLA", "aa_hexdigest": "2aedf62b5f480532fe37bba34bd7b74d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4158.8739000000005, "isoelectric_point": 4.830996131896971 }, "id": "EFPKPENHBP_830", "locus": "GCA000186005_00802" }, { "type": "cds", "sequence": "contig_1", "start": 446634, "stop": 447200, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C", "UniRef:UniRef90_UPI000FF5D93C" ], "nt": "ATGAAAGAAAAAATCTTACTGTTGGACGTCTTAGATAAAGAATATTTATCTGACGCAGTTTATAATCATGAAACAAAAATTTTAGAAATAAAAACAAAAAACCAAAGTTTTAGTGATCAATATTTAAATCACTTGTTTAATTATTTTGAATCAATTAGTCATGATGAAAAGAAAGAAAAAAATGACCAAGCTAAATATTTATTTATTGGTTATTTTGACTACGCAAAAAACAAATATGATGGCAGCGATGAAAGCAGAAAAAAAATAGTTACAAAAAGTAACAATGTAAACAAATATTGTTCTTATGTTAGAGTGCTTGATTTAAATATTGAATACAAACAAGATTCAAAAGAAATCGATAGATATAAACTTAAAGTAAAAGGAATTGGACGTTATAAAATTACTCATTTAGAAGATAATAGTGAAAAAACACCTGAAAATTCATATAATGTTGAATTTGCAGTTGCTTCAAAAAGTTCTTATGAAAATAAATTAAGTAAAGATTCAGTTAAAAAATTAAATGTATTAGATAAAACTATTACAAACTTAGTTGAAAAAGGATTATGA", "aa": "MKEKILLLDVLDKEYLSDAVYNHETKILEIKTKNQSFSDQYLNHLFNYFESISHDEKKEKNDQAKYLFIGYFDYAKNKYDGSDESRKKIVTKSNNVNKYCSYVRVLDLNIEYKQDSKEIDRYKLKVKGIGRYKITHLEDNSEKTPENSYNVEFAVASKSSYENKLSKDSVKKLNVLDKTITNLVEKGL", "aa_hexdigest": "538bdbb1a9a3e4b5b4c67d5328ad9018", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF5D93C", "query_cov": 1.0, "subject_cov": 0.9082125603864735, "identity": 0.9890000000000001, "score": 361.0, "evalue": 9.85e-126, "valid": true, "uniref50_id": "UniRef50_UPI000FF5D93C" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5D93C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22128.702400000016, "isoelectric_point": 8.606029701232913 }, "id": "EFPKPENHBP_831", "locus": "GCA000186005_00803" }, { "type": "cds", "sequence": "contig_1", "start": 448212, "stop": 449615, "strand": "+", "frame": 3, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "nt": "ATGTCCTTATTTAAAGAAGATGAAAAAAATGCTAAATTACAAACAGAATTTAACAATGTTCCTATTCTTACTTTAGTTGGTCCTCCAGGTACTGGTAAAACCTCTTTAGCCAGAGCTATTGCTGAAGCGCTTAACAAAAGTTACATTAAAATTAGTCTTGGTGGTGTGCATGATGAAAGTGAAATTAGAGGTCACAGACGTACTTATGTAGGTTCAATGCCAGGTAAAATTATTAAAGCTATCCAAAGAGCTGGTGTATCTAACCCACTTATTTTATTGGATGAAATTGACAAAATGTCATCTGATTATAAAGGTGATCCAGCTAGTGCAATGCTTGAAGTATTAGATCCTGAACAAAACACAAAATTCCAAGATAACTATCTTGAACATGAATATGACTTATCAAAAGTTATGTTTATTGCAACTGCTAACTACTACGAAAATATTCCTGCTCCTTTAATTGACCGTGTGGAAATTATCGAATTAAACTCATATACAATTAATGAAAAAATTAAAATTGCACGTGAACACTTAATTGATTCTACAATTCAACAAGCAGGTCTTAAAAAAGAACAATTCAATATTGATGATAAAACACTAGAATTTATTATCAAACACTATACAGCTGAAGCTGGTGTTCGTGGACTTAAGAGAGATTTAGATAAAATTGCTCGTAAAATAGTTACAAGAATTGTTGAAGGTGAAAAAATTGCTAAATTTACAATAACAAAAGATATTGTTACTGAAATGTTAGGTACTCCTAAATTTACAGATCACCAAGATACGAAAGAACCTCAAGTTGGAGTTGTTAACGGTTTAGCTTACACTTCAATAGGTGGAACAACATTGCAAATCGAAGTTTCAAGCTTCAAGAGTAAAAATAGCGGTATCAAATTAACCGGTTCGCTTAAAGATGTTATGAAGGAATCAGCCAATATTGCTCTTTCATATGTAAGAGCTAATGCTGAAAAATTTGATATTAAAGATTTTGCTTTTGATGAAAGAGAAATTCATATTCACGTTCCAGAAGGAGCTGTTCCTAAAGATGGGCCTAGTGCTGGTGTAACATTTACAACTGCTTTAGTTAGTGCTTTAAGTAATAAACCTGTACCTCAAGATATTGCAATGACAGGTGAAATTACTTTAAGAGGTCGTGTTTTTGAAATTGGTGGATTAAAAGAAAAATCATTTGCTGCATATAAAAAAGGAATCAAAACTGTATTTATTCCAAAAGACAATGAAAAGAATTTAGCAGATATTCCTGAAGAAGTTAAAAAAGTAATTACCTTTATTCCAGTTGAAACTTATGAAGAAATTTATAATCACATTTTTAAAGATCAAAAGCCTACAACTGATAAAAGCGATTCGACTAAAAAGTCTATAAAACAGGCAAATAAGAAATAA", "aa": "MSLFKEDEKNAKLQTEFNNVPILTLVGPPGTGKTSLARAIAEALNKSYIKISLGGVHDESEIRGHRRTYVGSMPGKIIKAIQRAGVSNPLILLDEIDKMSSDYKGDPASAMLEVLDPEQNTKFQDNYLEHEYDLSKVMFIATANYYENIPAPLIDRVEIIELNSYTINEKIKIAREHLIDSTIQQAGLKKEQFNIDDKTLEFIIKHYTAEAGVRGLKRDLDKIARKIVTRIVEGEKIAKFTITKDIVTEMLGTPKFTDHQDTKEPQVGVVNGLAYTSIGGTTLQIEVSSFKSKNSGIKLTGSLKDVMKESANIALSYVRANAEKFDIKDFAFDEREIHIHVPEGAVPKDGPSAGVTFTTALVSALSNKPVPQDIAMTGEITLRGRVFEIGGLKEKSFAAYKKGIKTVFIPKDNEKNLADIPEEVKKVITFIPVETYEEIYNHIFKDQKPTTDKSDSTKKSIKQANKK", "aa_hexdigest": "3c79830e44e2da6bc89de95234376dbf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0U7", "query_cov": 0.9271948608137045, "subject_cov": 0.8694779116465864, "identity": 0.674, "score": 572.0, "evalue": 7e-200, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A3B0P0U7", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "product": "endopeptidase La" }, "genes": [], "id": "EFPKPENHBP_832", "locus": "GCA000186005_00804" }, { "type": "cds", "sequence": "contig_1", "start": 450116, "stop": 450385, "strand": "-", "frame": 3, "gene": null, "product": "dUTPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33", "UniRef:UniRef90_C4XFC3" ], "nt": "TTGCACTTTGTTGGCTCTTTTGCTTATCAATATGAAGTTGATCCTAACATCACACCATTAATAATGAGTAAGGATGTTAATAAACAAATTTGCTTATTATTCCAAACAATTTCTTCAGCAACAAATAACATCAATAAATATGTTATTGGAGAAATATTAGCTTTAACTTTAGGGGTTGCTAAATTATTAGGTTACAGTCAAAAAGATCTTTTAGAAGCTTATTACAACAAAAACAAAAAGAATTACGAACGCATTAAAAATAAATATTAG", "aa": "MHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY", "aa_hexdigest": "36cb5dfe1e264b4a6a1c29ae44be49fc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 450116, "stop": 450652, "observations": { "mutation": [ 450442, 450625 ], "stop": [ 450442, 450625 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC3", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.9890000000000001, "score": 175.0, "evalue": 1.7e-54, "gene_start": 1, "gene_end": 89, "reference_start": 90, "reference_end": 178, "reference_sequence": "MALLNLLYNWNMNLTKIFTMQKDLDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVETAEFANEVQTFKYWKAKKQIDKDKALEEFADILHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY" }, "paralog": false, "identity": 0.9831460674157303, "score": 341.0, "evalue": 2.45e-122, "description": "Internal stop codon at 450442, 450625. Nonsense mutation around 450442, 450625.", "psc": { "uniref90_id": "UniRef90_C4XFC3", "product": "dUTPase", "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC3", "UniRef:UniRef50_N9VF33" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33" ], "product": "dUTP diphosphatase" } }, "id": "EFPKPENHBP_833", "locus": "GCA000186005_00805" }, { "type": "cds", "sequence": "contig_1", "start": 450440, "stop": 450619, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGAATTTAACAAAAATATTTACAATGCAAAAAGATCTTGACAAAGCTATTGCAGCTCGTAATGATATTGATGCAAAGACTTCAAAAAGCAAAATTAAAGAATTTCACTACATGAAATTATTAGCTTTACTTGTTGAAACGGCAGAATTTGCAAATGAAGTCCAAACATTCAAATATTGA", "aa": "MNLTKIFTMQKDLDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVETAEFANEVQTFKY", "aa_hexdigest": "c21748c1ffa36f76db06dbac1a3d977f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 58, "aa_cov": 0.9830508474576272, "hmm_cov": 0.896551724137931, "evalue": 3.9171639866620147e-13, "score": 46.32384490966797, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6855.9497999999985, "isoelectric_point": 9.004702186584474 }, "id": "EFPKPENHBP_834", "locus": "GCA000186005_00806" }, { "type": "cds", "sequence": "contig_1", "start": 450676, "stop": 451230, "strand": "-", "frame": 1, "gene": null, "product": "HEAT repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4", "UniRef:UniRef90_C4XFC4" ], "nt": "ATGGATGAAGAAAGTGTAGATGCATTATTAAAACGATTAGAAAAAGAAGACAAACTTTATACTAAAATCGCTATTTGTGAAACTTTAGAAACAGGTAATGCTTTTACAGCTAATAAAATGATTGAATATTTGAGCAAAATTGGTAATAACCAAATTAAAGAATTGCCTAAATATATTAGTAATAAAAGTTCTTATCCACTACCAAGAGACATTATTGCCAAATCTTTAGCTAAAATGCATACTTCAATTTTTCCTCTTTTACTTGAAACTCTTAAGCAAGGAACAATTGAACAAAAACAAGAAGTTATTGATGCAATTGGTTACATTTGTTTTAATAAAAAAGAATTAGCTACTGAAAAACTTTTAAATGAGCTAATTGATGTTTATGGTCAAAATATTGATAATAAAATTTTTGTTTGGAAATTTTTAACATTATTATCAGCTTTTAATTTTGAAGCAAGTAAAGAAATACTTAACTCATATTTAAATAGCAAAGATACTTTATCAAAAGAAGCAACTCACTCTTTAGATATATTAAAGGAAGCAAATATTTAG", "aa": "MDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI", "aa_hexdigest": "0537e01ee56c60b62ad7e6c83d2e8095", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 450676, "stop": 451431, "observations": { "mutation": [ 451266, 451362 ], "stop": [ 451266, 451362 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC4", "query_cov": 1.0, "subject_cov": 0.7330677290836654, "identity": 1.0, "score": 356.0, "evalue": 3.78e-123, "gene_start": 1, "gene_end": 184, "reference_start": 68, "reference_end": 251, "reference_sequence": "MSQIKAHFYIKRILMKPVENKKQWLSRGYVDQEDIAEFENKSKKELLELLQHEYAWVRSSAAYNLSPMDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI" }, "paralog": false, "identity": 0.9920318725099602, "score": 482.0, "evalue": 2.44e-175, "description": "Internal stop codon at 451266, 451362. Nonsense mutation around 451266, 451362.", "psc": { "uniref90_id": "UniRef90_C4XFC4", "product": "HEAT repeat domain-containing protein", "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC4", "UniRef:UniRef50_C4XFC4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4" ], "product": "HEAT repeat domain-containing protein" } }, "id": "EFPKPENHBP_835", "locus": "GCA000186005_00807" }, { "type": "cds", "sequence": "contig_1", "start": 451823, "stop": 451921, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGGAGATCTATGTCAACAGAAAACAAAACAGAATCTTTAAACAAAGTAAACAATGAAAAATTGGAAGCAAAAGAATTAAGCTATACTAAATGA", "aa": "MKRRSMSTENKTESLNKVNNEKLEAKELSYTK", "aa_hexdigest": "5d5edf307d0e1169c7525b30cfa8f4ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3788.2687000000014, "isoelectric_point": 9.600390052795408 }, "id": "EFPKPENHBP_836", "locus": "GCA000186005_00808" }, { "type": "cds", "sequence": "contig_1", "start": 452035, "stop": 452178, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATCTTAATTATAGGATTCTTCGTTGGAATATTAACATTCATCGGAGCATTTATTTATATTTCTGATGCTCAAAAAGAAAATAAAAACTACTTCAATTATTTTAAATCAGATGAATCAATTGACTCAAAAGCAGAATAG", "aa": "MIILIIGFFVGILTFIGAFIYISDAQKENKNYFNYFKSDESIDSKAE", "aa_hexdigest": "33a6840712a18ef3e6ea85f4642cdb96", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5415.169400000001, "isoelectric_point": 4.748352241516113 }, "id": "EFPKPENHBP_837", "locus": "GCA000186005_00809" }, { "type": "cds", "sequence": "contig_1", "start": 452296, "stop": 452454, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGAAAAAATCCTAAAAGTTCAGCTATTGCTTTGATTGTTTTATCACTTTTAAGCGCTGTTATAACAGGAATTGCAATATTTATGTTTATTGATGTTGCAGTTAAAATATTTAAAAGTGGAGAAGAAACAAATGAATATATTAAAAATAACTGA", "aa": "MHRKNPKSSAIALIVLSLLSAVITGIAIFMFIDVAVKIFKSGEETNEYIKNN", "aa_hexdigest": "9c018b39b53836b8fc329e9044cc8e2f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5736.745, "isoelectric_point": 9.302868461608885 }, "id": "EFPKPENHBP_838", "locus": "GCA000186005_00810" }, { "type": "cds", "sequence": "contig_1", "start": 453116, "stop": 453313, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTAAATTAGCATCTATTTCTAAAGCTTTTTCGAAAACTTCTTTGGTTAAGTCAATAGCACAAACTATACTACGTAAAGGTTCTGATAAATTGAATTTAATAGAAAAACCACTTGGGTCTCATTCTTCTTTAAGATCAAGTGGTCACTTTTCTAATAAATATTTTGTTACTTTTCTAATTTGCATTATTTAA", "aa": "MMIKLASISKAFSKTSLVKSIAQTILRKGSDKLNLIEKPLGSHSSLRSSGHFSNKYFVTFLICII", "aa_hexdigest": "e26b4431c9406e3ce81c146386714cbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.508499999996, "isoelectric_point": 10.302902030944821 }, "id": "EFPKPENHBP_839", "locus": "GCA000186005_00811" }, { "type": "cds", "sequence": "contig_1", "start": 453306, "stop": 453809, "strand": "-", "frame": 2, "gene": null, "product": "sigma-70 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "nt": "ATGGTTGAAACTATTAACAAAATCACTAAAATTGAACGTGAATTGCAACAAGAATTAGGTTATGAACCAACTGATGAAATGATTGCTGAAAGATATGGCAATGGCTATGACGCTGAAAAAGTTAGATACATTAGAAAAATCAACATTGACCCTATTTCTTTAGACAAACAAATTGGTAAAGAAAATGATTCATCATTTAGTGACTTTGTTAAAGATGAAAGTGTTGTTAATCCAGTTGATTATGCTTCACAAGAAGAATTAGTGGAAACACTAAATTCAATTCTTGAAAAATCACTTGATGATGATGAAAGAATTTTAATCTGCAAACGTTTCGGAGTTGGAGTTGACGAAAATGGAAATAAATACCCAATTCACTCACTTGAAAAATTAGCAGAAGAAAGAAATGTTTCTAAAGAAAGAATTCGTCAAATTGAAAACAAAATTTTAAGAAAACTTAAAAGTAGCACACGTAAAGGACGTCACTTGAAAGATTTCATTAAATAA", "aa": "MVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK", "aa_hexdigest": "d8910b176a042d5196fe307d3e10f9a7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF345E0", "query_cov": 0.8922155688622755, "subject_cov": 0.9933333333333333, "identity": 0.591, "score": 181.0, "evalue": 8.13e-56, "valid": false, "product": "sigma-70 domain-containing protein", "uniref50_id": "UniRef50_UPI002FF345E0" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF345E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "product": "sigma-70 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_840", "locus": "GCA000186005_00812" }, { "type": "cds", "sequence": "contig_1", "start": 453876, "stop": 454082, "strand": "-", "frame": 2, "gene": null, "product": "sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6", "UniRef:UniRef90_UPI0021E0C4F6" ], "nt": "ATGGAAAAAGGTGGCTTTAGAGGAAAACGTGCTAGGGATAAACTTATCAAACGTAATTTACGTTTAGTTATCAATAATGCTAAAAAATACAAAAATAGAGGACTTAGCTTTATTGACTTAATCTCAGAAGGTAACTCAGGTATCGTAAAAGCAGTTTCAAAATATAACGTTTCAAAAGGCTTTAAATTCTCAACATATGCAACTTGA", "aa": "MEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYAT", "aa_hexdigest": "ed6a4481227ac60f778d6860788d7ef0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 453804, "stop": 454128, "observations": { "insertion": [ 454086 ], "mutation": [ 454110 ], "stop": [ 453875, 453878, 454110 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "query_cov": 1.0, "subject_cov": 0.591304347826087, "identity": 0.868, "score": 117.0, "evalue": 7.01e-33, "gene_start": 1, "gene_end": 68, "reference_start": 19, "reference_end": 86, "reference_sequence": "MERWKTSRWCWRKKLCNEIEKGGFRGKRARDKLVLRNLRLVINNAKKYKNRGLSFIDLISEGNQGIMKAVQKFDVSKGYKFSTYATWWIRQAITRAVADQARTIRVYLYIYRNNK" }, "paralog": false, "identity": 0.7850467289719626, "score": 159.0, "evalue": 1.19e-51, "description": "Internal stop codon at 453875, 453878, 454110. Frameshift due to insertion around 454086. Nonsense mutation around 454110.", "psc": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021E0C4F6", "UniRef:UniRef50_UPI0021E0C4F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6" ], "product": "sigma-70 family RNA polymerase sigma factor" } }, "id": "EFPKPENHBP_841", "locus": "GCA000186005_00813" }, { "type": "cds", "sequence": "contig_1", "start": 454152, "stop": 454775, "strand": "-", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8", "UniRef:UniRef90_C4XFC8" ], "nt": "ATGAATAAAGCAAATGAAATTAAATCAATAATCTCAATGATTAAAAAACATGCAAAAAGCTTAAAGAAAAAACAAATAAGTCAGTCTGAAGTTTATGATTATTTAGATTCAATTAAAGTTGAAATTGATGATGAATCAATGGACGAAGTTTTAGGCCAACTTTTTGAATCAGGCTTAATTCTTGATGAAAGCGACGAGGGCGATTTAGACGATGTTAGTTTAGATGAATTAGACTCAGAATTAGAATTCGAAGAAAAAAGCAAAAAAGACGAAAACACCGATGAAATGGATGTTAAAAACAAAAAAAGTGATTTATATGACGAAGATTTAGATGAAGATGATAACTACAATTTAGTTGGTTCATATGGTGACGATGATGAAAGTCAAGAATACATTATTGAAGATGACTATGTTGATTCAAGTTATGATGAAGATGACGAAGATGAAGACGAAGAAGAATTAAGCAGTAAGAAAAAATCGAAAAAAAGTAAAAATAATGAAGATGAAGATTCACTTGAACTTGAATCTTATGAAAATGAAGAAATTAACCTTAATGATAACAAAGTAATTAAAGATAAAGATTTAACCAACAAACTTACCGAAACTAATGATATTGTTAAATGA", "aa": "MNKANEIKSIISMIKKHAKSLKKKQISQSEVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVK", "aa_hexdigest": "250567412293912e59135d44f9265a0f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 453849, "stop": 454775, "observations": { "mutation": [ 453875, 453878, 454142, 454154 ], "stop": [ 453875, 453878, 454142, 454154 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC8", "query_cov": 1.0, "subject_cov": 0.4233128834355828, "identity": 1.0, "score": 308.0, "evalue": 9.79e-101, "gene_start": 1, "gene_end": 207, "reference_start": 1, "reference_end": 207, "reference_sequence": "MNKANEIKSIISMIKKHAKSLKKKQISQSEVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVKWYMRWIGKYGKLLTIEEEKKLAQEMEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYATWWIRQAITRAVADQARTIRVPVHMVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK" }, "paralog": false, "identity": 0.987012987012987, "score": 525.0, "evalue": 3.89e-188, "description": "Internal stop codon at 453875, 453878, 454142, 454154. Nonsense mutation around 453875, 453878, 454142, 454154.", "psc": { "uniref90_id": "UniRef90_C4XFC8", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC8", "UniRef:UniRef50_C4XFC8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8" ], "product": "RNA polymerase sigma factor" } }, "id": "EFPKPENHBP_842", "locus": "GCA000186005_00814" }, { "type": "cds", "sequence": "contig_1", "start": 454768, "stop": 455118, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTCGGTAAATTAAATAATGAAGATTTAAAAAAAGAACTTTATCAAACATTTAGAGAAAGTAAAAGTATTAAGGTTGCTAAATTAAAGGAATATATCAAAGACTTAGAACAGGAAAATGTTGATATCGATTTAAATCAATTTTCAAAAAAATACAAAGAGGATAAAATTATTAAACAAAACTTTAAAGATGTTTATCGCCGAGCATTAAAGGAATCAGATAATGCTTATAATAAATACACAAGCAATCCTGAAGTTTTGAAACAAATCAGCAGTATCAAAAAAATGTATGATGAACACAAAGAAAAATTAAGAGAAATGCAAAAAAGAAAGGAAGAAAATGAATAA", "aa": "MFFGKLNNEDLKKELYQTFRESKSIKVAKLKEYIKDLEQENVDIDLNQFSKKYKEDKIIKQNFKDVYRRALKESDNAYNKYTSNPEVLKQISSIKKMYDEHKEKLREMQKRKEENE", "aa_hexdigest": "9d84694ae9e99a902a594380dcf948f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14195.017899999986, "isoelectric_point": 9.18708324432373 }, "id": "EFPKPENHBP_843", "locus": "GCA000186005_00815" }, { "type": "cds", "sequence": "contig_1", "start": 455203, "stop": 455838, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13662.12" ], "nt": "ATGGGTACAGCTTTAACCAATAATCATTTCCGATTACTAAAAAACAAAAAAGTAGTCATCTTTTTAGATGGTGATGATGCTGGTCAAAAAGCTTCACTAAAAAGTGCTAAGTTTTTATTAGACCATCGTATTGACACTTACATAGTACGTAACACAACTAAATATGACCCCGATGAATTATTTAATAGTTATGGTGCCGATATTCTTCACAAGTTAATTAAAGAAGCTCCAGCTGCATTAGATTTTATTTATGAAAATTTAAGCAAGAAATATAATCTCAGTGTTTCGCCAAACAATGAAATCAAAAATGTTAAAGCTTTTTGCAATGACTTTGTTAACTTCCTTGAACACCAAGAAATTAATGTTCAAGAATTCTACAAAAACAAAATTCAAACCGATTATGGTTTTAGCATTCAAGTAAAACACAACTTTAAGTTGCCTGATCCTTATGATTCTCAAGAAATTTATGCACCACAACAATTTCCAGAAGACTCAGGTGAATATCAATTATTAGAAAACAATATTCCCCCTTATGCTTATGATGAACAAAATTATAATCAAGTAGTTGAATACAGCAATAATGTCAATAATTTTAATCACCCTTTAAATAACGAAGTGCCATTAAGCGATTATTGA", "aa": "MGTALTNNHFRLLKNKKVVIFLDGDDAGQKASLKSAKFLLDHRIDTYIVRNTTKYDPDELFNSYGADILHKLIKEAPAALDFIYENLSKKYNLSVSPNNEIKNVKAFCNDFVNFLEHQEINVQEFYKNKIQTDYGFSIQVKHNFKLPDPYDSQEIYAPQQFPEDSGEYQLLENNIPPYAYDEQNYNQVVEYSNNVNNFNHPLNNEVPLSDY", "aa_hexdigest": "b73073e19d5197eec1d296505d8a2b58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13662.12", "name": "Toprim_4", "length": 56, "aa_cov": 0.22274881516587677, "hmm_cov": 1.0, "evalue": 2.792407063332767e-07, "score": 27.045278549194336, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 24562.979999999996, "isoelectric_point": 5.014642906188963 }, "id": "EFPKPENHBP_844", "locus": "GCA000186005_00816" }, { "type": "cds", "sequence": "contig_1", "start": 456439, "stop": 456675, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGAGTAATAATAACCATGAAATAGTTGACTCAATTATTGCAAGTAATGATATTGTTGATGTTATCAACTCATTTGTTACTTTAAGCAAAAAAGGTCGTAGTTTTGTTTGCATTTGTCCTTTTCACCAAGACACAAATCCATCAATGAATGTCGACCCTAAAAAGCAAATATTTAAATGCTTTGTCTGCCAAGTTGGTGGTAATGTTTTAACATTTTTAATGAAATACAAAAAATGA", "aa": "MSNNNHEIVDSIIASNDIVDVINSFVTLSKKGRSFVCICPFHQDTNPSMNVDPKKQIFKCFVCQVGGNVLTFLMKYKK", "aa_hexdigest": "279c97a7e20988a35d5eda3ed4f4a468", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 73, "aa_cov": 0.9358974358974359, "hmm_cov": 1.0, "evalue": 1.4944588035251754e-28, "score": 94.85169219970703, "start": 6, "stop": 78 } ], "seq_stats": { "molecular_weight": 8798.178199999998, "isoelectric_point": 8.781061363220214 }, "id": "EFPKPENHBP_845", "locus": "GCA000186005_00817" }, { "type": "cds", "sequence": "contig_1", "start": 456709, "stop": 457167, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGAAAGCTACTGGTGAAAACTTAGAATATGTTGACCCTGTAACAAACGAAAAATTTGTTCCTTTTGTTATTGAACCTTCAATAGGACTTGACCGTTTAATGTTAGCAATTTTAATTGATTCATATGACGAAGAAAAACTTGAAAAAGATGACTCAAGAGTTGTTTTACATCTACACAAAAATTTAGCTCCTTATCAAGTTGCAGTTTTACCTTTAGTTAAAAAACTAAATGATAAAGCAACAGAAGTTTATGAAAAATTACTAAAAGCAGGCTTATCTGTAACTTATGATGAAACTGGATCAATAGGCAAAAGATACCGTCGTCAAGATGCTATTGGTACACCTTATTGTGTTACAATAGACTTTGATTCACTTGAAAAAAATACTGTAACAATTAGAGACAGAGACAGTATGAAACAAAAAACTGTACTAATTAATAATTTACTAAAAGAACTATAA", "aa": "MKATGENLEYVDPVTNEKFVPFVIEPSIGLDRLMLAILIDSYDEEKLEKDDSRVVLHLHKNLAPYQVAVLPLVKKLNDKATEVYEKLLKAGLSVTYDETGSIGKRYRRQDAIGTPYCVTIDFDSLEKNTVTIRDRDSMKQKTVLINNLLKEL", "aa_hexdigest": "f97b55e36458c04480f1374f6be2bb55", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.993421052631579, "subject_cov": 0.8830409356725146, "identity": 0.57, "score": 173.0, "evalue": 9.79e-53, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 91, "aa_cov": 0.5723684210526315, "hmm_cov": 1.0, "evalue": 2.7029393965454448e-27, "score": 91.13040924072266, "start": 66, "stop": 152 } ], "seq_stats": { "molecular_weight": 17329.781799999997, "isoelectric_point": 5.477766990661621 }, "id": "EFPKPENHBP_846", "locus": "GCA000186005_00818" }, { "type": "cds", "sequence": "contig_1", "start": 457219, "stop": 457623, "strand": "-", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "ATGTTTGAAACCAAACAAGGAGTTACAGAAGAAACCAAAAACAAAATTTACTTACGTCCTGAAACTGCTCAAGGTATTTTTGTTAACTTTAAAAACGTACAAAGAAGCATGCGTTCAAAATTACCTATGGGCATAGGGCAAGTTGGAAAAAGTTTCAGAAATGAAGTAACACCAGGTAACTTTATTTTCCGTACTCGTGAATTTGAACAAATGGAACTTGAAGTATTTTGCCATCCAAAAGAAGCAAACAAACTTTACAAATACTACATTAAAAAAGCTGAAAAATTTGCTGAATATTTAGGCTTAAGCAAAGAAAATGTTCGTATCAGAGAACATGAAAAAGAAGAACTAAGTCATTATTCTTCAGGCACAAGTGACATTGAATATCTTTTCCCTTTTGGCTGA", "aa": "MFETKQGVTEETKNKIYLRPETAQGIFVNFKNVQRSMRSKLPMGIGQVGKSFRNEVTPGNFIFRTREFEQMELEVFCHPKEANKLYKYYIKKAEKFAEYLGLSKENVRIREHEKEELSHYSSGTSDIEYLFPFG", "aa_hexdigest": "8d4028caa58c5e3eefa13a1b12699004", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8582089552238806, "subject_cov": 0.8273381294964028, "identity": 0.6, "score": 145.0, "evalue": 3.18e-42, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "EFPKPENHBP_847", "locus": "GCA000186005_00819" }, { "type": "cds", "sequence": "contig_1", "start": 457966, "stop": 458070, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATAATGAAAAAAATATGACTACTTTAATAAATCATTTAAAAGCTAGTGGATTTGTTTATCAAGGTAGTGAAATTTATGGTGGTTTAGCTAATACTTGA", "aa": "MKNNEKNMTTLINHLKASGFVYQGSEIYGGLANT", "aa_hexdigest": "051db83d179e4d9698cbf2e553135eee", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.2002, "isoelectric_point": 8.161391258239746 }, "id": "EFPKPENHBP_848", "locus": "GCA000186005_00820" }, { "type": "cds", "sequence": "contig_1", "start": 458150, "stop": 458719, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGTTTTAGCTGCTGAAAAATCTTCTAAAACTAAAAAACAACCTAAAACTGAAACTATAGTTACTGAAGTGCCTCCAGTAGTTGAAGAAGTTAAAGATGAATCAGTTGTTGAAGCTGAAGCTGTAGTTGAAGAAGTTAAACCAGTTGAAGAAAAACAACCAACAATTAAAGAAGAACCGCTTATTGTTTTAGCTCCTGAAGAAGCTAAAAAAGAAAAAGTTGAAGACAATGATTTTGTCTTAATTGACCATAAAAAAGCTGAAAAACCTAAAACAAGAGACTTTGCTGTTAGAGTTGTTGAAGAAAAACCTATTCAAGTAATTGAAGAAAAAGAAGCTCAACCTGAAGATAATTTCTACACAGGAATTAGAGGTAATGAACCAGTTGCTCTAGAATCAGAAGAATCATCAAACAATGTTTCAACTAAGAAAAAACGTTCAGCAGGATTCTACTTCTTAATCTTATTATTAGTACTAATTCTTGCAGGTCTTTTAACCTTAGATATTCTTGCTATTATTCAATTAGCAGGTGGTTATACATTCTTTAATTTTGGTGCTTAA", "aa": "MEEAVLAAEKSSKTKKQPKTETIVTEVPPVVEEVKDESVVEAEAVVEEVKPVEEKQPTIKEEPLIVLAPEEAKKEKVEDNDFVLIDHKKAEKPKTRDFAVRVVEEKPIQVIEEKEAQPEDNFYTGIRGNEPVALESEESSNNVSTKKKRSAGFYFLILLLVLILAGLLTLDILAIIQLAGGYTFFNFGA", "aa_hexdigest": "bcf4e3f85d9e51ff9a607d5f6a1ffabb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21064.854000000007, "isoelectric_point": 4.723740959167479 }, "id": "EFPKPENHBP_849", "locus": "GCA000186005_00821" }, { "type": "cds", "sequence": "contig_1", "start": 458894, "stop": 459313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTCAATGAAATGGCTCGTAAAATGGAACTTTTAAGACAAAACTACAAGCCACTTGAAGGTGTAACATACATGAACTACATCACATTAAGTTTTGATGACTATGTAGGTGGTGTTGCTTTATACACAGAAAAAATTAAAAATATTATTGAAACAACTCCAAAACAAAATATTAGTGAAGCTGATTACAATAATATTAAGAAAAACTATGCTAACTTCAATAAACAATTATTAGAAGTTGAAGCAAATCCTAACTTCCCACATGCTGATTTAGTTCAAAAAATGCATGCTGAATTAAGTACAACTTTTGAATCATTACTTAGTTTATTAGTCTTAGATCCTAAATTAGAAGATACACCAGCTAACCGCGGTTATTTCCTAAATAAAGAAACTAAGCAAAAACAAGAAATCTCATGA", "aa": "MNFNEMARKMELLRQNYKPLEGVTYMNYITLSFDDYVGGVALYTEKIKNIIETTPKQNISEADYNNIKKNYANFNKQLLEVEANPNFPHADLVQKMHAELSTTFESLLSLLVLDPKLEDTPANRGYFLNKETKQKQEIS", "aa_hexdigest": "573070d8fb7684ef898c1a37d418e986", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16130.149199999983, "isoelectric_point": 5.434739875793456 }, "id": "EFPKPENHBP_850", "locus": "GCA000186005_00822" }, { "type": "cds", "sequence": "contig_1", "start": 460054, "stop": 460278, "strand": "+", "frame": 1, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGACCAAAAAAATTGCTAAAAAATCAAATATTTTTGCATTTTTTCTTATTTTTTTCCATTTAATTATTAATATAAACATAAAGTTTAAGTATAATAAAAACACCATGAATCATAAAGGCGATATTTTATTCGGAAAAATAAAAAGTGTAAGTAGTGAAGGACTTACAATTTTATGCAATAATGATTATTCTTATTTTATTCCTCGTGATTTGATAACTGACTGA", "aa": "MTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITD", "aa_hexdigest": "3302a32d0eb16ac32954932f9fb56dd5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 460012, "stop": 460518, "observations": { "mutation": [ 460276 ], "stop": [ 460276 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44047619047619047, "identity": 0.986, "score": 149.0, "evalue": 1.03e-44, "gene_start": 1, "gene_end": 74, "reference_start": 15, "reference_end": 88, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.39e-122, "description": "Internal stop codon at 460276. Nonsense mutation around 460276.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "EFPKPENHBP_851", "locus": "GCA000186005_00823" }, { "type": "cds", "sequence": "contig_1", "start": 460291, "stop": 460518, "strand": "+", "frame": 1, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGCGTCATGAATTTAAGATCGGCGAAAAAATTAATTTTATAGTTGAAAAAGTTGATTATCACAGTCATAGTGGTATTGGTAATTTTAAAATTAATCATGCCACCTTTTCACGCAGTCCATTTAAAGAAAATTTAAGCAATACAAAACATGGTTTTGAAAACTTGAAAAACCAAATTGATCAAGAAATCAAAAATTATCATAAAGAAGGTAATAATGGCAACAATTAA", "aa": "MRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN", "aa_hexdigest": "8887cc25eed6748d55227d3c5a208f77", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 460012, "stop": 460518, "observations": { "mutation": [ 460276 ], "stop": [ 460276 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 0.987, "score": 149.0, "evalue": 1.53e-44, "gene_start": 1, "gene_end": 75, "reference_start": 94, "reference_end": 168, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.44e-122, "description": "Internal stop codon at 460276. Nonsense mutation around 460276.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "EFPKPENHBP_852", "locus": "GCA000186005_00824" }, { "type": "cds", "sequence": "contig_1", "start": 460505, "stop": 461215, "strand": "+", "frame": 2, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHM8", "UniRef:UniRef90_C4XFD3" ], "nt": "ATGGCAACAATTAATTTGAAAGCTTATAATTATTCACCTGATTTTAAAAATGAAAGTTTGATTAATCGTGGCAATAACTTGATTAAGGATGTTAAATTAAAAAATATCGAGGGTTTTGAACATTTTGGTTTTCATGAATTAGCTTTAAATTTTAGTAATTTTAATGTTGATGAATTAAACAATTTTGTACGCTTAATAAAAAAGCAAGAAATTAAAGAAATTTTGATTATGACAACAAGCAAAATAATTGATAATTATCAAGCTGGAAATGACTTTTTATTTAAGTATGATTTATTAAAAAAATACAAGTTAAAATTTAATTTTATTAATGTTGATAATTATGAGCAAGATTACTATGCTAAGTTTGAAGAGTATAAGAAAAAATTTGAACAAAAAGGCATTGCTTTAATGTTTGGATCATTTACTAAATTTAGTGAAGAAACAATTAATTTTATTAAATTATTAATGAATCATTTTCAATTAAATCAAGGTTATTATCGAGCACTTGAAAGAGTTTTTTTAATCTCAAAAGAATCATTAGAAAAACAATTGGAATTTTTAAGTATTCCCCTTCAAAACAGACTTATTAGTCCAAATATCCTCTCAAATAATTTTGCTTTTTTTAGTGAAATTAATATGTTACTTTTGTTGCTTCAAGGAGCTAATATTAATGACTTATCTGAAGGCTATTCTTCAGGTTGCACAAACTGA", "aa": "MATINLKAYNYSPDFKNESLINRGNNLIKDVKLKNIEGFEHFGFHELALNFSNFNVDELNNFVRLIKKQEIKEILIMTTSKIIDNYQAGNDFLFKYDLLKKYKLKFNFINVDNYEQDYYAKFEEYKKKFEQKGIALMFGSFTKFSEETINFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTN", "aa_hexdigest": "043d74a416c8e1991b8d051ddb05fc79", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 460505, "stop": 461518, "observations": { "mutation": [ 461213 ], "stop": [ 461213 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD3", "query_cov": 1.0, "subject_cov": 0.5700483091787439, "identity": 1.0, "score": 464.0, "evalue": 1.58e-162, "gene_start": 1, "gene_end": 236, "reference_start": 1, "reference_end": 236, "reference_sequence": "MATINLKAYNYSPDFKNESLINRGNNLIKDVKLKNIEGFEHFGFHELALNFSNFNVDELNNFVRLIKKQEIKEILIMTTSKIIDNYQAGNDFLFKYDLLKKYKLKFNFINVDNYEQDYYAKFEEYKKKFEQKGIALMFGSFTKFSEETINFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTNWLSSNLEQNKAFQYAYIRYSLKGDRKNDLLIGTDERFKGILKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLYWSYIFECYLNKLNPFDI" }, "paralog": false, "identity": 0.9970326409495549, "score": 667.0, "evalue": 4.47e-245, "description": "Internal stop codon at 461213. Nonsense mutation around 461213.", "psc": { "uniref90_id": "UniRef90_C4XFD3", "uniref50_id": "UniRef50_A0A3P8LHM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD3", "UniRef:UniRef50_A0A3P8LHM8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHM8" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "EFPKPENHBP_853", "locus": "GCA000186005_00825" }, { "type": "cds", "sequence": "contig_1", "start": 461333, "stop": 461698, "strand": "+", "frame": 2, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4", "UniRef:UniRef90_C4XFD4" ], "nt": "TTGAAGCTTTATAGCACAATAAATAATAGTCAATATTTAAAGTCAAATAATGTGACAATGTATGCAATTTTTAGTCAAGATATTTATACTTATGGACAATATTTAATTGATAATAATCGCAACTTTTTTACCTCAATTTTTAGCATTGAAAATGAAAAAATTGACTTTAGAATTAGTGATGAAATTAATAATACTCAAGATGGTTTAGAGAACCATAAAGATAACCGCATAAGCAGCTATTACAAAGTAGCAAAAAAAGGTTATTTGACAACACTTTCAAATGTTGCATCATTACCAGTTTTTGATATTTCATTGGAAAGTTCAAATGAATTAAATTTAGGATACTTAGTTGGATTTCTATATTGA", "aa": "MKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLY", "aa_hexdigest": "cc5ee58e5096f7b663262de3093daad5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 461033, "stop": 461749, "observations": { "mutation": [ 461213, 461696 ], "stop": [ 461213, 461696 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD4", "query_cov": 1.0, "subject_cov": 0.4548872180451128, "identity": 0.992, "score": 236.0, "evalue": 9.96e-77, "gene_start": 1, "gene_end": 121, "reference_start": 129, "reference_end": 249, "reference_sequence": "MNFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTNWLSSNLEQNKAFQYAYIRYSLKGDRKNDLLIGTDERFKGILKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLYWSYIFECYLNKLNPFDI" }, "paralog": false, "identity": 0.9915966386554622, "score": 466.0, "evalue": 6.53e-170, "description": "Internal stop codon at 461213, 461696. Nonsense mutation around 461213, 461696.", "psc": { "uniref90_id": "UniRef90_C4XFD4", "product": "Glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD4", "UniRef:UniRef50_C4XFD4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "EFPKPENHBP_854", "locus": "GCA000186005_00826" }, { "type": "cds", "sequence": "contig_1", "start": 461858, "stop": 461980, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAGTTAAAGTTAATTTACATAATTGTGTTGATGAAAAAACATTACTTTCTTATCAAGACAAAGTTAAACAAATTCATGATTCAATAATGAACAAAACTATTGCAGAAAAAGATTGA", "aa": "MKAVKVNLHNCVDEKTLLSYQDKVKQIHDSIMNKTIAEKD", "aa_hexdigest": "c5ee275feb6609528749c64ef5df2ee1", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4629.3396999999995, "isoelectric_point": 7.901132392883301 }, "id": "EFPKPENHBP_855", "locus": "GCA000186005_00827" }, { "type": "cds", "sequence": "contig_1", "start": 462134, "stop": 462682, "strand": "+", "frame": 2, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.9", "GO:0004347", "GO:0006096", "SO:0001217", "UniRef:UniRef50_Q4A5S9", "UniRef:UniRef90_A0A3P8LAA1" ], "nt": "ATGTATGGACCTTACAGCATTAACAAACCAAAAATGGAATTATTATTTGCTGGAAATAGCATTAGTAGTGAAAGCTTAGTAGCAGCATTAAAATATGTTGAAAACAAAAAGTTTGCAATTAATGTTATTTCAAAAAGTGGAACAACTTTAGAACCTTCTATTGCTTTTAGAGAATTTAGAAAATTATTAGAAGCAAAAGTTGGAGCAGTAGAAGCTAAGAATTTAATTGTGGCTACTACTGATGCTAAAAAAGGCGTTTTATACTCATTAGCAACTCAAAAAGGATATGAAAAATTAATTATTCCTGATGATGTTGGTGGACGTTTTAGTGTTTTAAGTCCAGTTGGTTTATTCCCATTCATTTGTGCGGGAATTGATATTGAACAAATTCTTAAAGGTGCTCAAGAAGCTAATAAAGATGCAAATGATTTAAGTTTAAAAACAAATGATGCTTATCGTTATGCGGTTGCAAGACATGTTTTAAGTAAAAATTACACCATGGAAATGATGATTTCTTATGAGCCAAAAATGCAATATTTTGCAGAATGA", "aa": "MYGPYSINKPKMELLFAGNSISSESLVAALKYVENKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLATQKGYEKLIIPDDVGGRFSVLSPVGLFPFICAGIDIEQILKGAQEANKDANDLSLKTNDAYRYAVARHVLSKNYTMEMMISYEPKMQYFAE", "aa_hexdigest": "10efbc395c6977cd16e1c7548848d97a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 461858, "stop": 462985, "observations": { "mutation": [ 461978, 461987, 462053, 462680, 462683 ], "stop": [ 461978, 461987, 462053, 462680, 462683 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LAA1", "query_cov": 1.0, "subject_cov": 0.4262295081967213, "identity": 0.945, "score": 339.0, "evalue": 6.19e-114, "gene_start": 1, "gene_end": 182, "reference_start": 93, "reference_end": 274, "reference_sequence": "MKAVKLNLHNCVDEKLLLSYEDKVKSIHNSIMSKSVVEKDWLGWLELPNKAGSDEVKKMKKIVELWESKNIEAVVVIGIGGSYLGAKSGYEFIYGQYSINKPKMELVFAGNSISSESLVAALKYVEGKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLASQKGYEKLIIPDDVGGRFSVLSPVGLFPFLCAGIDIDQILSGAQAANKDANDLSLKTNDAYRYAVARHILSKNYTMEMMISYEPKMQYFAEWWKQLFAESEGKDGKGLFPTSGIFSTDLHSLGQMIQDGNKVLFETILVLDKPKYDIDFKADKEDLDKLVYLDKNKLHSVNMTAFNATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILKG" }, "paralog": false, "identity": 0.9093333333333333, "score": 667.0, "evalue": 8.57e-246, "description": "Internal stop codon at 461978, 461987, 462053, 462680, 462683. Nonsense mutation around 461978, 461987, 462053, 462680, 462683.", "psc": { "uniref90_id": "UniRef90_A0A3P8LAA1", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_Q4A5S9", "go_ids": [ "GO:0004347", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LAA1", "EC:5.3.1.9", "UniRef:UniRef50_Q4A5S9", "GO:0004347", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5S9" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "EFPKPENHBP_856", "locus": "GCA000186005_00828" }, { "type": "cds", "sequence": "contig_1", "start": 462779, "stop": 463138, "strand": "+", "frame": 2, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "nt": "ATGATTCAAGATGGAAATAAAGTATTATTTGAAACAATTTTAGTTTTAGATAAACCAAAATACGATATTACTTTTAAAGCTGATAATGAAGATTTAGATAAACTTGTTTATTTAGATAAAAACAAATTGCATAATGTTAACATGACAGCTTTTACAGCAACTTTAGATGCTCACATGAACACAGGTAAAGTTCCAAACATTGTAATGAATTTTGCAAAATTTGATGAATACACATTAGGTTACTTATTCCAATTCTTTGAAAGAGCTTTAGCTATTAGTGCTTATCTTTTAGGTGTTAATCCATTCAACCAACCAGGTGTTGAAGTTTATAAAGCTAATATGTTTAAAATTCTTAAATAA", "aa": "MIQDGNKVLFETILVLDKPKYDITFKADNEDLDKLVYLDKNKLHNVNMTAFTATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILK", "aa_hexdigest": "0ccad641ba841aa1870ecf5ded61c6ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A847B363", "query_cov": 0.9831932773109243, "subject_cov": 0.841726618705036, "identity": 0.513, "score": 111.0, "evalue": 2.88e-29, "valid": false, "product": "glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_A0A971BB97" }, "pscc": { "uniref50_id": "UniRef50_A0A971BB97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "product": "glucose-6-phosphate isomerase" }, "genes": [], "id": "EFPKPENHBP_857", "locus": "GCA000186005_00829" }, { "type": "cds", "sequence": "contig_1", "start": 463321, "stop": 463422, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAAAAGAAATGTATTATGAAAGATTATTAGATCCAATAATAAGAAAATATTTAAAAGTTTTTGGAGCAATTTGCATTCAAGGACCTAAATGA", "aa": "MKYKKEMYYERLLDPIIRKYLKVFGAICIQGPK", "aa_hexdigest": "37118d86afd9c251cbf486223585ce72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4036.9117000000015, "isoelectric_point": 9.666663551330569 }, "id": "EFPKPENHBP_858", "locus": "GCA000186005_00830" }, { "type": "cds", "sequence": "contig_1", "start": 463642, "stop": 463857, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGGTTCTTCTACTCCTGAACATAAAGGCCTATTTCATAGTGGTATTGGTCGAATTGCTACATTACAATTGAGACCTATGAGCTTATTTGAATCTAAAGATTCTTTAGGACAAATTTCATTAAAAGATTTAATAAATGACAATTTTGAAAATACAACTTATGAAGGTATTGAATTAAATAAAATTATTAATTTAATTATTAGAGGTGGTTGA", "aa": "MTGSSTPEHKGLFHSGIGRIATLQLRPMSLFESKDSLGQISLKDLINDNFENTTYEGIELNKIINLIIRGG", "aa_hexdigest": "e72666246ead2cdd8db9f28b1014b4e9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7831.846499999996, "isoelectric_point": 6.034504890441894 }, "id": "EFPKPENHBP_859", "locus": "GCA000186005_00831" }, { "type": "cds", "sequence": "contig_1", "start": 464134, "stop": 464334, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGCAACCATTTAGTTTGAATATTAGATCCAATTTAAGAATTAAACAAAATGAAAAAAGACTTTTTGTCGATCCTTCTTTAGCAGCTGCGTTATTGAATGTTAATAATGAGAGTTTGCAAAATGATTTAAATACTTTGGGTTTTTTATTTGAAAACCTTGTCTTAAGAGATTTGAAAGTTTATGCTCAAAGTAATGAGTGA", "aa": "MQPFSLNIRSNLRIKQNEKRLFVDPSLAAALLNVNNESLQNDLNTLGFLFENLVLRDLKVYAQSNE", "aa_hexdigest": "62a5f12d2a6b060b2dd6002d34aa8ddc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 0.96875, "evalue": 2.4559281326741275e-12, "score": 43.17341995239258, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7591.5720999999985, "isoelectric_point": 6.178137016296386 }, "id": "EFPKPENHBP_860", "locus": "GCA000186005_00832" }, { "type": "cds", "sequence": "contig_1", "start": 465579, "stop": 465899, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAACCCTTCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTGCTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAACAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAAAATTTCTTCTTCAGTAATATCATCAAAATTAGTCTTTTTCTTAAAAAAATCTTCTAACTAA", "aa": "MRALTLPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKTDKYPKISLGRLFISFCTFKISSSVISSKLVFFLKKSSN", "aa_hexdigest": "d4b8debfab38e3e77715dbefa5e800f5", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12382.585999999981, "isoelectric_point": 9.913126182556148 }, "id": "EFPKPENHBP_861", "locus": "GCA000186005_00833" }, { "type": "cds", "sequence": "contig_1", "start": 466255, "stop": 467076, "strand": "+", "frame": 1, "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104", "KEGG:K10563", "RefSeq:WP_013526847.1", "SO:0001217", "UniParc:UPI00000B1D7B", "UniRef:UniRef100_C4XFD9", "UniRef:UniRef50_A0A974WKQ3", "UniRef:UniRef90_C4XFD9" ], "nt": "ATGCCAGAATTACCAGAAGTTAGAAGTGTGGTCAAAGATTTAAGACCTAAAGTTGTTAATAGAAAAATAGTTAAAATTGATATCTTACATCCTAAATTAATTAAAGAGGTAAGTGTTGAAGAATTCAAGAATTTTTTAATTAATGAAACATTTTTAGATGTCAATAATTTAGCTAAACATATTATTTTTTCTTTAACAAATAATAAATATTTATTAAGCCACTTGAGAATGAGTGGAAAGTATTTTACACATTATAAATATCGTCCAGCAACAAAACATGATTACTTAATTTTTCATCTTGATGATAATAGTTGTATTTATTATAATGACTCACGTCAATTTGGCACTTTTCATATAAAAACTAAGGGCACTTTATATACAACTAAACCTTTAGATAAAGTTGCAAAAATTCCTTCAGAAACAAATATTAAAGAGTTATTTAATAAAATTAAAAATAAGAATATACCTATAAAACAGCTTCTTTTGGATCAAAGCTTTGTTTCAGGTATTGGAAATATTTATGCTAATGAAACTTTATTTGCCACTCAAATTAATCCTTTGACGCCTTCAAAAAATATAACTTTTGAACAGTTAGAAAAAATTATTAAAGCAGCTGCAAAAATTATGGATCAAGCTACTGAATTAGGTGGTTCAAGCATTGACACATATACTTCAGTTGATGGAGTTAAAGGTCAATTTCAAGATTTTTTACAAGTTCATGGACACTTTAATGATGTATGTAAAAGATGTAAAAAAGCTAAAATTAATAAAATTTTTATTAACAAAAGAGGGACTTATTATTGTCCAAATTATCAAAAATAA", "aa": "MPELPEVRSVVKDLRPKVVNRKIVKIDILHPKLIKEVSVEEFKNFLINETFLDVNNLAKHIIFSLTNNKYLLSHLRMSGKYFTHYKYRPATKHDYLIFHLDDNSCIYYNDSRQFGTFHIKTKGTLYTTKPLDKVAKIPSETNIKELFNKIKNKNIPIKQLLLDQSFVSGIGNIYANETLFATQINPLTPSKNITFEQLEKIIKAAAKIMDQATELGGSSIDTYTSVDGVKGQFQDFLQVHGHFNDVCKRCKKAKINKIFINKRGTYYCPNYQK", "aa_hexdigest": "d8905f5119a432459c837c4c7f468128", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000B1D7B", "ncbi_nrp_id": "WP_013526847.1", "uniref100_id": "UniRef100_C4XFD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B1D7B", "RefSeq:WP_013526847.1", "UniRef:UniRef100_C4XFD9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFD9", "uniref90_id": "UniRef90_C4XFD9" }, "psc": { "uniref90_id": "UniRef90_C4XFD9", "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A974WKQ3", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104" ] }, "pscc": { "uniref50_id": "UniRef50_A0A974WKQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A974WKQ3" ], "product": "Bifunctional DNA-formamidopyrimidine glycosylase/DNA-(Apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "EFPKPENHBP_862", "locus": "GCA000186005_00834" }, { "type": "cds", "sequence": "contig_1", "start": 467109, "stop": 467417, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGTGTTCTAGAGATTTAAAAATATATTCATCAAGTTTAAATGGAGAATTATCATATTATCACGATAGATATGGTCTTGAAATTGATTTTGTTTTGCACTTAAATGATGGCAGATATGCATTGATAGAATGTAAATTAGGTGGAAATCAAATTGAAAAAGCGAAAAATAATTTATTAAAAGTAAAACAGCTGATAAAAGAAAATGGACAAAGAGAACCGGATCTTATGATTATTTTAACAGCAGAAGATAGAGCATATGAAATAACAGATGGAATACTTATTATTCCAATTAGTCTACTAAAAGATTAA", "aa": "MCSRDLKIYSSSLNGELSYYHDRYGLEIDFVLHLNDGRYALIECKLGGNQIEKAKNNLLKVKQLIKENGQREPDLMIILTAEDRAYEITDGILIIPISLLKD", "aa_hexdigest": "50c3ca6f07f0e61a10b35c2d9dfeaa70", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_G6AUJ2", "query_cov": 0.9019607843137255, "subject_cov": 0.968421052631579, "identity": 0.522, "score": 95.1, "evalue": 1.46e-23, "valid": false, "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_G6AUJ2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 36, "aa_cov": 0.3431372549019608, "hmm_cov": 1.0, "evalue": 1.673682831365206e-08, "score": 30.7131404876709, "start": 12, "stop": 46 } ], "seq_stats": { "molecular_weight": 11685.353499999987, "isoelectric_point": 5.352891731262207 }, "id": "EFPKPENHBP_863", "locus": "GCA000186005_00835" }, { "type": "cds", "sequence": "contig_1", "start": 467562, "stop": 468089, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAAAGGGGTTATTTATTTTAACAGGCTCAAGTACACACAATAAATTAAATACTATGCATACTGGTACTGGTAGAATACATACAATGAAAATGTATCCAATGAGTTTATATGAAACTGGAGAATCTAGTGGAAAAATATCTTTACAAGATTTATTTGATAAAAAAATTAAAGTAAATGGTTTAAAATCAAATTTATCTTTTGAAGATTTAGCAAAAGCAATTTGCAGAGGAGGATGGCCAAGTACTCTAAATTTAAAAAAATATGAAAATCAAATCGAAATTCCTAAAGATTATGTCAATCAAATTTGCAATGTAGATATAAGTAAAATTGATAACATAAAAAGAAGCCCTGAAATTACTAGAAGAATATTGAAAAGTTATGCAAGAAATGTTGCTACTTTAGCTACTTCAAAAACTTTAATAAAAGATTGTAGTTTTAAAGATGAAGGTTCAAAAACAACTTTTTATGATTACACTACAAAACTTGAAGATTTAAATGTTATTGATAATATAACTGCATGA", "aa": "MGKKGLFILTGSSTHNKLNTMHTGTGRIHTMKMYPMSLYETGESSGKISLQDLFDKKIKVNGLKSNLSFEDLAKAICRGGWPSTLNLKKYENQIEIPKDYVNQICNVDISKIDNIKRSPEITRRILKSYARNVATLATSKTLIKDCSFKDEGSKTTFYDYTTKLEDLNVIDNITA", "aa_hexdigest": "a60c6c17d3e853e2d6fa181ffec30ae7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19724.474400000006, "isoelectric_point": 9.331621360778808 }, "id": "EFPKPENHBP_864", "locus": "GCA000186005_00836" }, { "type": "cds", "sequence": "contig_1", "start": 468141, "stop": 468374, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAAGAAGAATAATAGAAAAACAAATAAAAAAAAATCTTGAAATTTTTGGTGCTATAAACATAGAAGGAATTAAACAATGTGGTAAAACAACTACTGCACAAAAATTTTCAAAAACATTAATTAAATTACAAGATCCAGACAATTTAAAAACTTACAAAGAAACAATTGAATATAAACCTTCATTACTTTTAGAAGGAGAAAAACCATTATTAATAGATGAATGA", "aa": "MKYKRRIIEKQIKKNLEIFGAINIEGIKQCGKTTTAQKFSKTLIKLQDPDNLKTYKETIEYKPSLLLEGEKPLLIDE", "aa_hexdigest": "ce4ef8650e664774e20486f27b1139a7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8968.482799999996, "isoelectric_point": 9.380681800842286 }, "id": "EFPKPENHBP_865", "locus": "GCA000186005_00837" }, { "type": "cds", "sequence": "contig_1", "start": 469077, "stop": 469397, "strand": "-", "frame": 2, "gene": null, "product": "Amino acid permease/ SLC12A domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2", "UniRef:UniRef90_C4XFE2" ], "nt": "ATGATTACAGTTATAATAAATTATTCTTTAATTGCTATTTTTGCAATTTTACATAATAGTGGAGTTGTTGAACAAATAATTAAAAATACAATTGATCAAAGAACAAATCAACATTTAGTGCAAAGTTTAAGCATTATTTTTAGTCTATTCTTATTTATTGCTGTTGTTGGAGTATTAAATGATTTTACCAATTCATATGCACATAATATTAATAATGCTATTAATTTAAAAATAATAATTAATACTACTAAAATAAGAAACAAAATGAATCTTAAAAGAATGATGATTTTAATTATAAGTCTTATTTATAGTGCTATTTGA", "aa": "MITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAI", "aa_hexdigest": "298fdedea63d1b0b8f16822e0146dae8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 469020, "stop": 469634, "observations": { "mutation": [ 469055, 469079, 469523 ], "stop": [ 469055, 469079, 469523 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE2", "query_cov": 1.0, "subject_cov": 0.5196078431372549, "identity": 1.0, "score": 187.0, "evalue": 2.13e-58, "gene_start": 1, "gene_end": 106, "reference_start": 80, "reference_end": 185, "reference_sequence": "MINDLNFSGVNLISLYNALSFNKTDQLKNTKTNGISNWATQLGNKKFCYFISFNYTFLSVGSTANNLKKDNLPTVAIVGMITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAIWLIISICIWVLGGDNILDG" }, "paralog": false, "identity": 0.9803921568627451, "score": 382.0, "evalue": 6.08e-138, "description": "Internal stop codon at 469055, 469079, 469523. Nonsense mutation around 469055, 469079, 469523.", "psc": { "uniref90_id": "UniRef90_C4XFE2", "product": "Amino acid permease/ SLC12A domain-containing protein", "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE2", "UniRef:UniRef50_C4XFE2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2" ], "product": "Amino acid permease/ SLC12A domain-containing protein" } }, "id": "EFPKPENHBP_866", "locus": "GCA000186005_00838" }, { "type": "cds", "sequence": "contig_1", "start": 470131, "stop": 470367, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCCACAATCTTTAGCTTTAGGTGTAATCATTGGACATATTTTTCCAGTTTACTTTAAATTTAAAGGTGGTAAAGGTGTAGCTTGTACAATTGGTTTAATTGCCACAATTAATATTGTCTTTTTATTAATGGCTTCTATTATCTTCTTTATAGTTGTCTTAACTTCTAAATATGTATCTTTAGCTTCAATATTAACAGCGGCAATATTAGTGCCCTTTGCTTTTATTCCTTGA", "aa": "MSPQSLALGVIIGHIFPVYFKFKGGKGVACTIGLIATINIVFLLMASIIFFIVVLTSKYVSLASILTAAILVPFAFIP", "aa_hexdigest": "8d66a0f3ff6af79c81325c8b74145ed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8288.133699999991, "isoelectric_point": 9.748087120056148 }, "id": "EFPKPENHBP_867", "locus": "GCA000186005_00839" }, { "type": "cds", "sequence": "contig_1", "start": 470404, "stop": 470577, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATCACAATTGCTTTGTAACAAGTTTTATCTATTTAATAGCTGCAATATTAATTATTGCAATGCATCATAGTAATATTACTCGTTTAATTAATCATACAGAAAGTAAATTAGGCCAAAAAAAAAGCGAAGATAATGGTATGAACCCCAAAAATTGGACTTTTTAA", "aa": "MYEYHNCFVTSFIYLIAAILIIAMHHSNITRLINHTESKLGQKKSEDNGMNPKNWTF", "aa_hexdigest": "1dd8db8e89a7398194bfd61a15d52852", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6685.6443, "isoelectric_point": 7.951030921936035 }, "id": "EFPKPENHBP_868", "locus": "GCA000186005_00840" }, { "type": "cds", "sequence": "contig_1", "start": 470853, "stop": 471410, "strand": "-", "frame": 2, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_869", "locus": "GCA000186005_00841" }, { "type": "cds", "sequence": "contig_1", "start": 471476, "stop": 471766, "strand": "-", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_870", "locus": "GCA000186005_00842" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 472052, "stop": 472153, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 54.3, "evalue": 8e-09, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TGAAATTGACAGAGGTATTTGCTTATATAACAAGCAGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTGAATT", "id": "EFPKPENHBP_46" }, { "type": "cds", "sequence": "contig_1", "start": 472205, "stop": 472510, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATATATAAATTTATTGTAGCAAGTTCAAGTATTTTTGCTTTAGGAAGTATTAGTGCTGGTTGCCAATATCAACCTAATATTGAAGAAATAAAAATTAAAATGGAAGGTATTAAATATGCAGATAAAGAAAAGTTAGATGCATATACAAAAGTATTTAATCAAGTTTTTAATATGAAAATGAAAGAAGCAAATTTAAATCAAAAAGTTGTTTCGTTTCATGATTCAGGAAATGGTGAAAGCGCAACACTTAAAGAAGTTAATTTAAACAAATTTCAAGTGGCTTTTCCCGGAGCTTGA", "aa": "MKKIYKFIVASSSIFALGSISAGCQYQPNIEEIKIKMEGIKYADKEKLDAYTKVFNQVFNMKMKEANLNQKVVSFHDSGNGESATLKEVNLNKFQVAFPGA", "aa_hexdigest": "438388db6a770fa377e83dcdf6951637", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11302.961399999991, "isoelectric_point": 9.187276649475098 }, "id": "EFPKPENHBP_871", "locus": "GCA000186005_00843" }, { "type": "cds", "sequence": "contig_1", "start": 472529, "stop": 472714, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATAAAAATAAAAATGTTGTAGCTTTTATGCAAACAAAAACTAGAGCTTTTAAAACAAAAAGTAGTTTAAATTACAATGATCAAAGTCTTTATGATCCAAGTAAAAGTAATAACTTCGCAACTTTACAAGCTCAATATGAAAATGAATTATTCAATAGTGAAGGTGGCTATGTAAATTGA", "aa": "MNNKNKNVVAFMQTKTRAFKTKSSLNYNDQSLYDPSKSNNFATLQAQYENELFNSEGGYVN", "aa_hexdigest": "817667c37054e92ce57c53d97b033df4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7004.609200000002, "isoelectric_point": 9.049507713317869 }, "id": "EFPKPENHBP_872", "locus": "GCA000186005_00844" }, { "type": "cds", "sequence": "contig_1", "start": 473036, "stop": 473305, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCAAGGATAATTCTTCAAATTATCATATAGCTTTTGATAATGAGAATTCTTTTGCTTATAGTAATCATGGAAATAAAGCATTAGAAAATAGAGATTTTTATGTTAAAGATAAAAATAAAACTTATGAAATGTTAACTTTAACTGATCCTATTCCTTATAATGTGGCTATAGCAAATAATTCACTAAGTAAAAATGTATTAGAATTATTAGCTCAAACTTATATTGAATTAGCAAATAAAAATAATAATTATGTTGGAAATTTATGA", "aa": "MGKDNSSNYHIAFDNENSFAYSNHGNKALENRDFYVKDKNKTYEMLTLTDPIPYNVAIANNSLSKNVLELLAQTYIELANKNNNYVGNL", "aa_hexdigest": "764a55c8c98340f759798a3f323c0fc6", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10138.073699999995, "isoelectric_point": 5.528865242004394 }, "id": "EFPKPENHBP_873", "locus": "GCA000186005_00845" }, { "type": "cds", "sequence": "contig_1", "start": 473385, "stop": 473726, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE5", "UniRef:UniRef90_C4XFE5" ], "nt": "ATGAAAACATACAAAATTGAATTTAAAAATGTAACTTTAGCTTATCAAAATGAAGTGGTCTTAAGCAATTTAAATATCATTTTTGAAAGTGGCAAAACTTATGGAATTTATGGGCTTAGTGGGGTTGGGAAAAGTACTTTGATTAAAGCTATTTTAGAACCAAAATTAATAGCTAAAGGGCAAATATTAATTAATGATCAAGATATCAATAAAATTGATCAAAAGACCTATAAAACAGTTAAAAAAGATATTTCTTTTTTATTTCAAGAGCCTTCTTTAATTGAGACAGATGATGTCATTGAAAATATTAAAAGAAAGTCAAATAATATCTATAATAATTGA", "aa": "MKTYKIEFKNVTLAYQNEVVLSNLNIIFESGKTYGIYGLSGVGKSTLIKAILEPKLIAKGQILINDQDINKIDQKTYKTVKKDISFLFQEPSLIETDDVIENIKRKSNNIYNN", "aa_hexdigest": "e20d82acec63dbe603376cdd4b243d43", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 473376, "stop": 474029, "observations": { "mutation": [ 473724 ], "stop": [ 473724 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE5", "query_cov": 1.0, "subject_cov": 0.452, "identity": 0.938, "score": 199.0, "evalue": 1.63e-62, "gene_start": 1, "gene_end": 113, "reference_start": 6, "reference_end": 117, "reference_sequence": "MWNNNMKTYKIEFKNVTLAYQNEVVLAFKYHFWSGKTYGIYGLSGVGKSTLIKAILEPKLIAKGQILINDQDINKIDQKTYKTVKKDISFLFQEPSLIETDDVIENIKRKSNNIYNNWFYKVFRILTKEQKNKLAKILEYLDIFDYINTPVKDLSGGQKQRVELASIFFENKNIILADEPTSNLDFINTKKILKYLQKYVHDNNALLLCNIHDVTNSLEYMDYILLLKNKRLVFIKQANNISEKEFQEQY" }, "paralog": false, "identity": 0.9631336405529954, "score": 405.0, "evalue": 4.3e-146, "description": "Internal stop codon at 473724. Nonsense mutation around 473724.", "psc": { "uniref90_id": "UniRef90_C4XFE5", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE5", "UniRef:UniRef50_C4XFE5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE5" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_874", "locus": "GCA000186005_00846" }, { "type": "cds", "sequence": "contig_1", "start": 473856, "stop": 474125, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACTAGCCTCAATCTTTTTTGAAAATAAAAATATTATTTTGGCTGATGAACCAACAAGTAATTTAGATTTTATTAATACTAAAAAAATATTAAAATATTTGCAAAAATATGTTCATGATAATAATGCATTATTATTATGTAACATTCATGATGTTACTAATTCGCTAGAATATATGGATTATATTTTATTATTAAAAAATAAGAGACTTGTTTTTATTAAACAAGCTAATAATATAAGCGAAAAAGAATTTCAAGAACAATATTAA", "aa": "MELASIFFENKNIILADEPTSNLDFINTKKILKYLQKYVHDNNALLLCNIHDVTNSLEYMDYILLLKNKRLVFIKQANNISEKEFQEQY", "aa_hexdigest": "76cb33609cef8b1a96b426de9eb5cd1f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10586.088499999993, "isoelectric_point": 5.592013359069824 }, "id": "EFPKPENHBP_875", "locus": "GCA000186005_00847" }, { "type": "cds", "sequence": "contig_1", "start": 474262, "stop": 474780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGCGAAAGTTATTCTATTTTCAAAGAATTACTAATTACTATCAAAATTACTATTGCCAGCATTTTCTTAGCTTTTATCTTTTCAATTTTTGCTTCTTTTTTTGCAAACGATAAAACAAATAATAAATTTGTGAAAATAATAAGTAAAATGCTTTTAGCATTCTTTAGAACTATACCAATAACCTTATTTTTCTATTTATCAATTAATCTTTATTATTCAAGAGAAGTCATTTTAATTTTAGCTTTATCTTTTACTACAATGCGTTCAATGAGTAAATTTTATACTGAATCAATTAATAATATTGATCAAAAATTAATTGGCAATTATTTATTATTAAATAAAAATAAAATAACTCTATATTTTGAATTTATTTTACCTAGAGTAATTAAAGATTTTTATAGTTATGCAGCTTTTAGATTCGAATTAATCTTTAGAAACATTATTAATTATGGAACTTTTGCAGCTGTTGGTTTAGGCGCTAGATTAAATTATTACCAAAACAAAAATGAATGA", "aa": "MKSESYSIFKELLITIKITIASIFLAFIFSIFASFFANDKTNNKFVKIISKMLLAFFRTIPITLFFYLSINLYYSREVILILALSFTTMRSMSKFYTESINNIDQKLIGNYLLLNKNKITLYFEFILPRVIKDFYSYAAFRFELIFRNIINYGTFAAVGLGARLNYYQNKNE", "aa_hexdigest": "2056a0524c234575a59fe4e34dc7d20a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20263.694399999993, "isoelectric_point": 9.74731349945068 }, "id": "EFPKPENHBP_876", "locus": "GCA000186005_00848" }, { "type": "cds", "sequence": "contig_1", "start": 475529, "stop": 475750, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACTTCATAAAAAACTTAAAGATAAAATTCATAAATTATTTGCGGGATATGAAGAGTTTATATTATTAAAAGTAAATAATCTTTGTAGATCATTTTTTAATTGTTTTACTGAATTATTGTCCATAATTTTTCAATCAAAAGATAATAATGAATATAAACAAATTATTAGAATAAGCAAAATAAAAGTTACTTTAACTCATCTTTTGAGTTTTTTATAA", "aa": "MVLHKKLKDKIHKLFAGYEEFILLKVNNLCRSFFNCFTELLSIIFQSKDNNEYKQIIRISKIKVTLTHLLSFL", "aa_hexdigest": "a96987788128ee145e4ed8e57011b5a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8718.346999999992, "isoelectric_point": 9.596844291687013 }, "id": "EFPKPENHBP_877", "locus": "GCA000186005_00849" }, { "type": "cds", "sequence": "contig_1", "start": 476126, "stop": 476257, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGGATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "EFPKPENHBP_878", "locus": "GCA000186005_00850" }, { "type": "cds", "sequence": "contig_1", "start": 476387, "stop": 476851, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCGCTTTGGTAACAAAAGTTATATGAGAGCTGTAAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTGGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHV", "aa_hexdigest": "ccb8803a02cc51d36329f58c8d23c026", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 476126, "stop": 476887, "observations": { "mutation": [ 476255, 476333, 476357, 476372, 476849 ], "stop": [ 476255, 476333, 476357, 476372, 476849 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.9940000000000001, "score": 286.0, "evalue": 3.25e-96, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9446640316205533, "score": 451.0, "evalue": 1.92e-163, "description": "Internal stop codon at 476255, 476333, 476357, 476372, 476849. Nonsense mutation around 476255, 476333, 476357, 476372, 476849.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17764.905099999993, "isoelectric_point": 6.913292503356933 }, "id": "EFPKPENHBP_879", "locus": "GCA000186005_00851" }, { "type": "cds", "sequence": "contig_1", "start": 476906, "stop": 477538, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAAGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTAACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCCTATAATTCAAATGCTGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCTAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLK", "aa_hexdigest": "eeab87916b5d962c26bbd893192ae7b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 476906, "stop": 477736, "observations": { "mutation": [ 477536 ], "stop": [ 477536 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 1.0, "score": 389.0, "evalue": 1.47e-135, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.967391304347826, "score": 508.0, "evalue": 1.04e-184, "description": "Internal stop codon at 477536. Nonsense mutation around 477536.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23689.56930000002, "isoelectric_point": 5.2369401931762685 }, "id": "EFPKPENHBP_880", "locus": "GCA000186005_00852" }, { "type": "cds", "sequence": "contig_1", "start": 478268, "stop": 478435, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEAYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "ffe7870c92ac45336f99773804f27cea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6172.882700000004, "isoelectric_point": 7.942263221740723 }, "id": "EFPKPENHBP_881", "locus": "GCA000186005_00853" }, { "type": "cds", "sequence": "contig_1", "start": 478547, "stop": 478765, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNNDLIKNYLENDYLSAVKDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "1d4319ff09a22acc5365a504b9799701", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8406.355899999999, "isoelectric_point": 4.6834421157836905 }, "id": "EFPKPENHBP_882", "locus": "GCA000186005_00854" }, { "type": "cds", "sequence": "contig_1", "start": 478747, "stop": 479022, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCACTAGTAGTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAAACTTTAGGTTTAGCTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAA", "aa": "MISLVVFPLSTPYRCHEYSALTLSPLEFPQPPFVLPFEYPPVSKSEIRSYRCFLPKTLGLAQIFDFSSQFALFCETDVGLYNFVGDQYTSP", "aa_hexdigest": "f6ce9c8dd16ef992161b08df3311427a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10396.849499999993, "isoelectric_point": 4.794903373718263 }, "id": "EFPKPENHBP_883", "locus": "GCA000186005_00855" }, { "type": "cds", "sequence": "contig_1", "start": 479204, "stop": 479410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAACGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATATAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "84a9c29ce6fbec573d274b5d361b5d54", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8060.188600000002, "isoelectric_point": 8.803109550476076 }, "id": "EFPKPENHBP_884", "locus": "GCA000186005_00856" }, { "type": "cds", "sequence": "contig_1", "start": 480359, "stop": 480526, "strand": "-", "frame": 3, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTATTCTCCTGAAATAAGAACGCTTATTTATACTACTAATCTAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTATTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSPEIRTLIYTTNLIENLNSVFRKYTKTKRVFPSDDSLLKIIFLASQQIIKK", "aa_hexdigest": "67e4356b6ed447bfc4d9282f75679d63", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 68.6, "evalue": 2.97e-14, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "EFPKPENHBP_885", "locus": "GCA000186005_00857" }, { "type": "cds", "sequence": "contig_1", "start": 480587, "stop": 480853, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCTGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAAGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGTAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQSIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDVKNAMDNLDIFEDK", "aa_hexdigest": "21cc45ab9b26762f583e32f8a16b7771", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 5.639421594685681e-08, "score": 29.49219512939453, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10173.664599999995, "isoelectric_point": 8.806848716735843 }, "id": "EFPKPENHBP_886", "locus": "GCA000186005_00858" }, { "type": "cds", "sequence": "contig_1", "start": 481034, "stop": 481171, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGTTTTATATTCTAGAGGAATGCCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTCGAAGATGTAAGATTATGA", "aa": "MIFVLYSRGMPTRDVSDFMFSKYGVNYSPAQISQLTNEIVEDVRL", "aa_hexdigest": "54078c20f8f59997e722d9839537b3cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5218.9333, "isoelectric_point": 4.8610639572143555 }, "id": "EFPKPENHBP_887", "locus": "GCA000186005_00859" }, { "type": "cds", "sequence": "contig_1", "start": 482093, "stop": 482299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "512f6d302678aef6e46dcbb1e9f8c22a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8002.109400000004, "isoelectric_point": 8.830702018737792 }, "id": "EFPKPENHBP_888", "locus": "GCA000186005_00860" }, { "type": "cds", "sequence": "contig_1", "start": 482735, "stop": 482953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNNDLIKNYLENDYLSAVKDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "1d4319ff09a22acc5365a504b9799701", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8406.355899999999, "isoelectric_point": 4.6834421157836905 }, "id": "EFPKPENHBP_889", "locus": "GCA000186005_00861" }, { "type": "cds", "sequence": "contig_1", "start": 483065, "stop": 483232, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCGAAATTAGCAACAATGGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLATMANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "49892cd94114844b2b217e714c8dfe30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.973100000005, "isoelectric_point": 7.942263221740723 }, "id": "EFPKPENHBP_890", "locus": "GCA000186005_00862" }, { "type": "cds", "sequence": "contig_1", "start": 483324, "stop": 483638, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAA", "aa": "MIKFIFSNSPLNFSLSFRISLFSIEDISSVDKSGLLLSISSIVFLTSSNWLNVFKLAKTFDKKSTIFGFALLISFEISLGFEAKLLTFCKFKSVAFLIILNISK", "aa_hexdigest": "6036e17d51597afc3f4d971a728c2a35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11676.756399999982, "isoelectric_point": 9.667566108703614 }, "id": "EFPKPENHBP_891", "locus": "GCA000186005_00863" }, { "type": "cds", "sequence": "contig_1", "start": 483813, "stop": 484082, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAATGCTTTTTTAAATCCACTTTTTATTGAATCTAATTCATTTTTGCTATTAACATATTCATTAATTAATTTGTTTCTGCTTTCAATATACTCATCTCATTTGAGAGAATTTAATTCAATTTTAGATTTTTGTACAAATTCATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTCGATTGAATCAACATTTGAATTATAA", "aa": "MFASNAKLNSFNLDLILTNAFLNPLFIESNSFLLLTYSLINLFLLSIYSSHLREFNSILDFCTNSSIICFELFSLANLIVVSIESTFEL", "aa_hexdigest": "ecdcb3573624772f90080294155e0366", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10110.622799999988, "isoelectric_point": 4.246577644348145 }, "id": "EFPKPENHBP_892", "locus": "GCA000186005_00864" }, { "type": "cds", "sequence": "contig_1", "start": 483962, "stop": 484594, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATCGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSSKSLTSIINGLNEFNSFVESSNQISFEKLDEFINKTQAILNDENNLTTSIQSVIKEFLNAYNSNVDSIETTIRLAKLNNSKQIIDEFVQKSKIELNSLK", "aa_hexdigest": "41330f4a34df5d8f73cbaf868ff1f701", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 483764, "stop": 484594, "observations": { "mutation": [ 483964 ], "stop": [ 483964 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9520000000000001, "score": 368.0, "evalue": 3.48e-127, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9347826086956522, "score": 493.0, "evalue": 1.27e-178, "description": "Internal stop codon at 483964. Nonsense mutation around 483964.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23724.532300000024, "isoelectric_point": 5.4200754165649405 }, "id": "EFPKPENHBP_893", "locus": "GCA000186005_00865" }, { "type": "cds", "sequence": "contig_1", "start": 484649, "stop": 485113, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCACAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAATGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTTDEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDNAADFIKSDFHV", "aa_hexdigest": "f0cfebd605a500edc678fbd9dc4709b7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 484613, "stop": 485374, "observations": { "mutation": [ 484651, 485128, 485143, 485167, 485245 ], "stop": [ 484651, 485128, 485143, 485167, 485245 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.922, "score": 268.0, "evalue": 9.24e-89, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8853754940711462, "score": 429.0, "evalue": 1.28e-154, "description": "Internal stop codon at 484651, 485128, 485143, 485167, 485245. Nonsense mutation around 484651, 485128, 485143, 485167, 485245.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17784.825299999993, "isoelectric_point": 5.740135765075684 }, "id": "EFPKPENHBP_894", "locus": "GCA000186005_00866" }, { "type": "cds", "sequence": "contig_1", "start": 485243, "stop": 485374, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "EFPKPENHBP_895", "locus": "GCA000186005_00867" }, { "type": "cds", "sequence": "contig_1", "start": 485625, "stop": 485792, "strand": "-", "frame": 2, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTACTCCCTTGAAATAAGAACGCTCATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATGCAAAAACAAAAAAGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKK", "aa_hexdigest": "d72a3c85770b93ad34e400e9ef47f997", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 65.9, "evalue": 3.47e-13, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "EFPKPENHBP_896", "locus": "GCA000186005_00868" }, { "type": "cds", "sequence": "contig_1", "start": 485820, "stop": 485945, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATTAGGCAAAAAATATCCTACATCTATTTCAATATGA", "aa": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDKLGKKYPTSISI", "aa_hexdigest": "01d869fb16f96e61ee6513b861c0995e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4729.3643, "isoelectric_point": 6.046952629089356 }, "id": "EFPKPENHBP_897", "locus": "GCA000186005_00869" }, { "type": "cds", "sequence": "contig_1", "start": 485947, "stop": 486105, "strand": "-", "frame": 1, "gene": null, "product": "transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83", "UniRef:UniRef90_UPI0028527D83" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGTTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATGA", "aa": "MSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVK", "aa_hexdigest": "4d2caef1bccf1cce25b0674586a48bbd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 485962, "stop": 486255, "observations": { "mutation": [ 486108 ], "stop": [ 486108 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0028527D83", "query_cov": 0.9038461538461539, "subject_cov": 0.47474747474747475, "identity": 0.915, "score": 89.4, "evalue": 4.19e-22, "gene_start": 1, "gene_end": 47, "reference_start": 51, "reference_end": 97, "reference_sequence": "MIYIDAVHFHVVDNNVVTKKATYVIMGINGEGQKEILGLYIGENESAKFWMSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIEI" }, "paralog": false, "identity": 0.9072164948453608, "score": 177.0, "evalue": 1.69e-59, "description": "Internal stop codon at 486108. Nonsense mutation around 486108.", "psc": { "uniref90_id": "UniRef90_UPI0028527D83", "product": "transposase", "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0028527D83", "UniRef:UniRef50_UPI0028527D83" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83" ], "product": "transposase" } }, "id": "EFPKPENHBP_898", "locus": "GCA000186005_00870" }, { "type": "cds", "sequence": "contig_1", "start": 486286, "stop": 486666, "strand": "-", "frame": 1, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFB2", "UniRef:UniRef90_C4XFB2" ], "nt": "ATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATAGGTTACGAAAAAATGATAATAAAGTTCAAAAAAAATTATAGAAATGATTTTTTTAAGAAAAATGTTAATAGTCAATATGGCCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCAAAAATTGAACCTATAATCATCAAGAAGTGTGAACGTGATATTTCTGAATTAGTTGATATGATATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAATGATTTTATGTTTAGCAAATATGGTGTTAATTATTCTCCAGCACAAATAAGTCAATTAACTAACGAAATTGTCGAAGACGCAAGATTATGA", "aa": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARL", "aa_hexdigest": "f68bd9483c5e921774c802e29186cde5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 485983, "stop": 486666, "observations": { "mutation": [ 486108, 486288 ], "stop": [ 486108, 486288 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB2", "query_cov": 1.0, "subject_cov": 0.49606299212598426, "identity": 1.0, "score": 249.0, "evalue": 5.66e-82, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARLWQERKLETYYSIIYIDAVHFHVVDNNVVTKKATYVIMGINSDGQKEILGLYIGENESTKFWMSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVKWYERILQRFKICISI" }, "paralog": false, "identity": 0.9911894273127754, "score": 443.0, "evalue": 7e-161, "description": "Internal stop codon at 486108, 486288. Nonsense mutation around 486108, 486288.", "psc": { "uniref90_id": "UniRef90_C4XFB2", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFB2", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB2", "UniRef:UniRef50_C4XFB2", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB2" ], "product": "Mutator family transposase" } }, "id": "EFPKPENHBP_899", "locus": "GCA000186005_00871" }, { "type": "cds", "sequence": "contig_1", "start": 487225, "stop": 487782, "strand": "-", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_900", "locus": "GCA000186005_00872" }, { "type": "cds", "sequence": "contig_1", "start": 488599, "stop": 489345, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0", "UniRef:UniRef90_C4XFB0" ], "nt": "ATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGA", "aa": "MYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLE", "aa_hexdigest": "d325320591f32ac6814f3d3679059197", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 488347, "stop": 489519, "observations": { "mutation": [ 488514, 488601, 489465 ], "stop": [ 488514, 488601, 489465 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB0", "query_cov": 1.0, "subject_cov": 0.6358974358974359, "identity": 1.0, "score": 491.0, "evalue": 3.51e-173, "gene_start": 1, "gene_end": 248, "reference_start": 59, "reference_end": 306, "reference_sequence": "MIMEGAVKNKMSKTLADNWNAQAEVFKNSEERKIKYNVERKIERDIFIWGETVRIIYYMYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLEWITKQEKNKTVYANAKKFKKYYKENREYIWNSVLVKNYFGGCAETVVGHDLKRFCSKKFATFSLKTIQLSIAKNMETKANLFFI" }, "paralog": false, "identity": 0.9897435897435898, "score": 768.0, "evalue": 5.17e-285, "description": "Internal stop codon at 488514, 488601, 489465. Nonsense mutation around 488514, 488601, 489465.", "psc": { "uniref90_id": "UniRef90_C4XFB0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB0", "UniRef:UniRef50_C4XFB0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0" ], "product": "ISLre2 family transposase" } }, "id": "EFPKPENHBP_901", "locus": "GCA000186005_00873" }, { "type": "cds", "sequence": "contig_1", "start": 489762, "stop": 490211, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_C4XFA9" ], "nt": "TTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGA", "aa": "MLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHND", "aa_hexdigest": "d11fea40fd11324cc7c0bad85465fa78", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 489561, "stop": 490502, "observations": { "mutation": [ 489764, 490268, 490472 ], "stop": [ 489764, 490268, 490472 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA9", "query_cov": 1.0, "subject_cov": 0.476038338658147, "identity": 0.993, "score": 280.0, "evalue": 6.88e-93, "gene_start": 1, "gene_end": 149, "reference_start": 98, "reference_end": 246, "reference_sequence": "MYNFYMSLELWRPLKITNPLLEDIFHLNIDTKFKIHPLIIFENTKNKTYYCIRLQTARKSSSKYNLIIDNSSYQTNEYWKNHESVVVTKDIFIIDKELLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHNDWKDLDKTKDNVNIYSFENIGFLSKSSKSESSYIDPYDNKNIEKAKDKVAKEIPIKESKTDKDKTKLK" }, "paralog": false, "identity": 0.987220447284345, "score": 610.0, "evalue": 1.67e-225, "description": "Internal stop codon at 489764, 490268, 490472. Nonsense mutation around 489764, 490268, 490472.", "psc": { "uniref90_id": "UniRef90_C4XFA9", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA9", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17230.18999999998, "isoelectric_point": 8.557549476623539 }, "id": "EFPKPENHBP_902", "locus": "GCA000186005_00874" }, { "type": "cds", "sequence": "contig_1", "start": 491077, "stop": 491226, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTTCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAA", "aa": "MNKKGFKAGKSKKQVSIKKFKEIQIYKKDRIKAKPVVLSDEEINKLFAK", "aa_hexdigest": "df2560211445a3f631e2ef9afa6784c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5709.8361, "isoelectric_point": 10.22006015777588 }, "id": "EFPKPENHBP_903", "locus": "GCA000186005_00875" }, { "type": "cds", "sequence": "contig_1", "start": 491644, "stop": 492336, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGA", "aa": "MRGTGKYINHSITFNLKNSAYHVYYPPIDLSSLDIQKHFEIEMGKNISNDSKELKEKIRNKLIGLIEEQFNLYKNKTGIEMLLNKDIKVEGLDNVLDLLVQGNKFKDGIDIKIKGINANLISSTKIHIINDTEAPAFDLSLLNDTLVKNPIVISQKEPEKITKDIINKLNEITIASGIEFKKDYLVSLVNASFYLLGLVADIDKLNEAKYEKFKKVYEETKAKVMAEYNT", "aa_hexdigest": "765f71697b058a00437b3fa17058fe4a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26199.983000000033, "isoelectric_point": 6.923068809509277 }, "id": "EFPKPENHBP_904", "locus": "GCA000186005_00876" }, { "type": "cds", "sequence": "contig_1", "start": 492439, "stop": 492606, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGA", "aa": "MNYGDETNEAIKENYRRQVKEIVLNFVKRNIANSAKLKAIGITENEINGGWVIAN", "aa_hexdigest": "81fb4d5b440c05c797eb70b5ddfcd8b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.0062, "isoelectric_point": 8.06539783477783 }, "id": "EFPKPENHBP_905", "locus": "GCA000186005_00877" }, { "type": "cds", "sequence": "contig_1", "start": 492631, "stop": 492729, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGA", "aa": "MISLYKNFVDQASKFNVETGNEYENIFKDKIT", "aa_hexdigest": "1f15fa2f5e0a93065b5e0a705db0eb3e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3787.208700000001, "isoelectric_point": 4.93575038909912 }, "id": "EFPKPENHBP_906", "locus": "GCA000186005_00878" }, { "type": "cds", "sequence": "contig_1", "start": 492811, "stop": 493275, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGA", "aa": "MFSDELKEQLRFVYPNIKITTDKADLGIYAEANVLGKGALRNLVVDKVLESSSRYYKCLLFRKENDKYVEIGRKEQKLIEIPVNNGTSEYSYMSEEGIWLLFTVNEKSISNVNLVLIDAKSEPKSFFLDSIENKIEKINNQSEDYFEAFLDGGN", "aa_hexdigest": "14f3e53a5efaf9d6cffd2a93f9497e12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17724.8618, "isoelectric_point": 4.860722923278809 }, "id": "EFPKPENHBP_907", "locus": "GCA000186005_00879" }, { "type": "cds", "sequence": "contig_1", "start": 493288, "stop": 493431, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGA", "aa": "MDQKQIISPYMLDSKGKELKDKEGKLIVNKNYDPSIDKETGTKKQIV", "aa_hexdigest": "819168f07f3459a3efeb395e0df65970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5396.195300000002, "isoelectric_point": 8.96376476287842 }, "id": "EFPKPENHBP_908", "locus": "GCA000186005_00880" }, { "type": "cds", "sequence": "contig_1", "start": 493447, "stop": 493722, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGA", "aa": "MVNKQKVSVLNRIFEKKLIDERVNVNDASKIPFDKNSGSGNGEGDVAKNEYLVEVYRYKKGSNNSGEPEEKYVVIYEVIGNQFKQNIKYYA", "aa_hexdigest": "57d3445016ece3a9d5ba8fc5ce158842", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10439.608, "isoelectric_point": 8.752115058898926 }, "id": "EFPKPENHBP_909", "locus": "GCA000186005_00881" }, { "type": "cds", "sequence": "contig_1", "start": 493798, "stop": 493998, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGA", "aa": "MYKRLSGASLASRLTISTSITNPDGSISQIEAKTKANGGDLKNLIKRDEPERVSNKELEKDFVGKP", "aa_hexdigest": "e3d98ceff45638283a1d2f30053658e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.081999999999, "isoelectric_point": 9.453788948059085 }, "id": "EFPKPENHBP_910", "locus": "GCA000186005_00882" }, { "type": "cds", "sequence": "contig_1", "start": 494125, "stop": 494475, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTCACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGA", "aa": "MNRFEYGVNKKIDQNHYSIDLLSDTLGDFTTSVDGVFKDNGGKSNIEHLNKIFDKEYEFEYASGIKVKYKYDYKIKNEAVGEKNKIDVYIKVLSVKIDDKEIIDKDLDKILNNIED", "aa_hexdigest": "3b51870aa20a7ab6eaf58d735c44552d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13509.014199999981, "isoelectric_point": 5.077279472351074 }, "id": "EFPKPENHBP_911", "locus": "GCA000186005_00883" }, { "type": "cds", "sequence": "contig_1", "start": 494518, "stop": 494769, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGA", "aa": "MEEHKGASFTNLFAEYKVSQKTLEFLNNLETDKDKRVYSGLTDDKNKMFLWGLHDFDCEIEGMYDVRYYNNKVLNNLEYAKVG", "aa_hexdigest": "9bda0e8721a59f6b7453f6ce3f3a7db8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9826.907499999998, "isoelectric_point": 5.06073932647705 }, "id": "EFPKPENHBP_912", "locus": "GCA000186005_00884" }, { "type": "cds", "sequence": "contig_1", "start": 494881, "stop": 495342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGA", "aa": "MHAEARAKNEEKMNKKKMLKLIPLSVLSIAIPSVLNLSLNTNKKLLGYGKDFKAPEEKHKIDTNSLFSNFTSMEDDEIAKNERLQNRYQVKWSQLCDKGLLYAQDQWVGYHKTGPVKNKITSIVSRPTLGSFEVRYEKAYYTNPSNHNSYGWF", "aa_hexdigest": "547e92dcc2bb3430ecf9e1292e14eeb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17690.019999999993, "isoelectric_point": 9.477900123596193 }, "id": "EFPKPENHBP_913", "locus": "GCA000186005_00885" }, { "type": "cds", "sequence": "contig_1", "start": 495388, "stop": 495783, "strand": "-", "frame": 1, "gene": null, "product": "ICEF Integrative Conjugal Element-II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7", "UniRef:UniRef90_C4XFA7" ], "nt": "TTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAA", "aa": "MTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK", "aa_hexdigest": "1e201de3510ce021ade96f9646eff4b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 495388, "stop": 496071, "observations": { "mutation": [ 495792, 496062 ], "stop": [ 495792, 496062 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA7", "query_cov": 1.0, "subject_cov": 0.5745614035087719, "identity": 0.992, "score": 242.0, "evalue": 2.31e-79, "gene_start": 1, "gene_end": 131, "reference_start": 98, "reference_end": 228, "reference_sequence": "MKTWEISQENKKGDKLYVKSSRASKNGSKCISTRLSEPDYVALYERWVKRIKQSGSTPYVELKDIIRNALIKQEKEEIAKDFKNDLFYALRKVSWASLTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK" }, "paralog": false, "identity": 0.9780701754385965, "score": 429.0, "evalue": 1.42e-155, "description": "Internal stop codon at 495792, 496062. Nonsense mutation around 495792, 496062.", "psc": { "uniref90_id": "UniRef90_C4XFA7", "product": "ICEF Integrative Conjugal Element-II", "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA7", "UniRef:UniRef50_C4XFA7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7" ], "product": "ICEF Integrative Conjugal Element-II" } }, "id": "EFPKPENHBP_914", "locus": "GCA000186005_00886" }, { "type": "cds", "sequence": "contig_1", "start": 496052, "stop": 496858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ], "nt": "ATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MDDLIKQMKKFVAPKDHHDKEGYTKKVLDMAELLEGKFGRYGTLGALYNGHTNINIENDLVVFKTSNLIDTEGSVNARIGIMVLISLVNGFIFDNAFNNKKRINEYKEKNKIRLISKKEIQKLTRYCSFCIDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "fd4e47e07cfb58f2a4c9ea07298295fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F2982C0", "query_cov": 0.9776119402985075, "subject_cov": 0.9225352112676056, "identity": 0.622, "score": 325.0, "evalue": 4.81e-109, "valid": false, "uniref50_id": "UniRef50_UPI001F2982C0" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2982C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30615.79740000003, "isoelectric_point": 8.78963565826416 }, "id": "EFPKPENHBP_915", "locus": "GCA000186005_00887" }, { "type": "cds", "sequence": "contig_1", "start": 496865, "stop": 497311, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGA", "aa": "MFSLTSSRANHNVMILGTSGMGKSTLTKKILTSSLAAGNIAIIIDPQAEYVKWANKMGGQIVDLGSGDGTVINPLQVKSFIAREEDDANVNFNTLINNHLLWLEKFFSFIFDDMNEMKKNILQLEIIKLYQSWGIYRLKEVKELKNDG", "aa_hexdigest": "7bdeadcb03d81e2d1d7547110e406fd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 49, "aa_cov": 0.3310810810810811, "hmm_cov": 1.0, "evalue": 6.643206648327057e-08, "score": 28.243488311767578, "start": 9, "stop": 57 }, { "id": "PF01935.23", "name": "DUF87", "length": 108, "aa_cov": 0.722972972972973, "hmm_cov": 0.7777777777777778, "evalue": 2.353833018396146e-10, "score": 37.21625900268555, "start": 12, "stop": 118 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 76, "aa_cov": 0.5135135135135135, "hmm_cov": 0.881578947368421, "evalue": 2.441558326642471e-08, "score": 29.528656005859375, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 16628.021899999992, "isoelectric_point": 6.732430839538575 }, "id": "EFPKPENHBP_916", "locus": "GCA000186005_00888" }, { "type": "cds", "sequence": "contig_1", "start": 497315, "stop": 497728, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGA", "aa": "MDAGNLDNSIINRFGSALEEESIAVMIDQIQADNFKLFDSNIFVLAEAENAEELKQNNLKIKNGFTRSNFGINDLTFRQFEGFLDMSNYPLSKLDREYYQITSINNAIGYPLKNLPLNDGNNLLLGNEVGGNDSPIV", "aa_hexdigest": "60571d7151425553be189582d3abe5bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15326.890099999975, "isoelectric_point": 4.264652442932129 }, "id": "EFPKPENHBP_917", "locus": "GCA000186005_00889" }, { "type": "cds", "sequence": "contig_1", "start": 497834, "stop": 498181, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGA", "aa": "MIETFYLILNAPTLEELDECYSTFIQKAEDYEVISYEKLSGFELLSFLNKLNHFNKSEEDIETFLNESDIYESEYSLDRLFKYEKVEFKSNSLKIDDKLYSVKCAGNIPIRLNNE", "aa_hexdigest": "7329907af2238aaf3f1e5143e18b2cfa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13622.117199999982, "isoelectric_point": 4.459382820129394 }, "id": "EFPKPENHBP_918", "locus": "GCA000186005_00890" }, { "type": "cds", "sequence": "contig_1", "start": 498236, "stop": 498400, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGATGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGA", "aa": "MVLIENFARILDSFKYKFSIVKLDEQIDYRRNLNYINTIQSKKMLNSGGETKEF", "aa_hexdigest": "7eabf1fafeeaa9103dfce5aa5be32540", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.395699999999, "isoelectric_point": 9.098954963684083 }, "id": "EFPKPENHBP_919", "locus": "GCA000186005_00891" }, { "type": "cds", "sequence": "contig_1", "start": 498422, "stop": 498625, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGA", "aa": "MFKFKAMPKKYSNVVDDKNVRDSSDLNPYSTLVENDVVKNKSFSNKLFQKSEHSNYFSVFKLGGKNI", "aa_hexdigest": "da513e353c81c917ec7655a69dc05219", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7754.720499999998, "isoelectric_point": 9.601872825622557 }, "id": "EFPKPENHBP_920", "locus": "GCA000186005_00892" }, { "type": "cds", "sequence": "contig_1", "start": 499152, "stop": 499592, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAA", "aa": "MNICVKNRFTAPKKAKSICSPRKNTPTDNIFPTYLSFHILTFLPSSSKLKGKIIGNIKYKKNNGNIFPMASIPPKTSGIAINTAPIIDRNAAVIKMDAQLPMFKCSVFFAKATKLPKLISKLIVKPKGFVVAGIKISLTSPANFGG", "aa_hexdigest": "be119545e9f8a00836c1c4d3d042829e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15852.893799999973, "isoelectric_point": 10.454209327697754 }, "id": "EFPKPENHBP_921", "locus": "GCA000186005_00893" }, { "type": "cds", "sequence": "contig_1", "start": 499445, "stop": 499756, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGA", "aa": "MRSALVFSVKSLFILLAFQITILLFNLFVQKIITLMIGEGNDIVVAFINGLHRLFYPPKLAGDVKDILIPATTNPFGFTINLDISFGSFVALAKKTEHLNIGN", "aa_hexdigest": "1f530bd3ff05364bbc363b2280c88bc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11381.43519999998, "isoelectric_point": 9.223765754699706 }, "id": "EFPKPENHBP_922", "locus": "GCA000186005_00894" }, { "type": "cds", "sequence": "contig_1", "start": 499823, "stop": 500071, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGA", "aa": "MSGFFTRVMEIFKYGPTYVLYLLLFFVSLIVFILLAILLSVVKLATFDLLNFVIFGIMPRQNILDHQSPYMYVRFAIVSAVI", "aa_hexdigest": "594dbf9addb063641ab0662d22f90068", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9502.483199999995, "isoelectric_point": 9.222605323791505 }, "id": "EFPKPENHBP_923", "locus": "GCA000186005_00895" }, { "type": "cds", "sequence": "contig_1", "start": 500161, "stop": 500352, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0395 family pilin-like conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4", "UniRef:UniRef90_C4XFA4" ], "nt": "ATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGA", "aa": "MKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKIL", "aa_hexdigest": "5087b9cbe03a20493b3e281279e3d943", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 500071, "stop": 500358, "observations": { "mutation": [ 500127, 500163 ], "stop": [ 500127, 500163 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA4", "query_cov": 1.0, "subject_cov": 0.6631578947368421, "identity": 1.0, "score": 117.0, "evalue": 4.48e-33, "gene_start": 1, "gene_end": 63, "reference_start": 3, "reference_end": 65, "reference_sequence": "MKMKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKILWAGIVLILVIVGWGIFELVISIQASGGKTS" }, "paralog": false, "identity": 0.968421052631579, "score": 172.0, "evalue": 2.82e-57, "description": "Internal stop codon at 500127, 500163. Nonsense mutation around 500127, 500163.", "psc": { "uniref90_id": "UniRef90_C4XFA4", "product": "Mbov_0395 family pilin-like conjugal transfer protein", "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA4", "UniRef:UniRef50_C4XFA4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4" ] } }, "id": "EFPKPENHBP_924", "locus": "GCA000186005_00896" }, { "type": "cds", "sequence": "contig_1", "start": 500524, "stop": 500730, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "EFPKPENHBP_925", "locus": "GCA000186005_00897" }, { "type": "cds", "sequence": "contig_1", "start": 500734, "stop": 501099, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEQVYRNYFKEYLDKATNALLDVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDNQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "013ce869a4b1cb22bf3d6b450ba5ea08", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14177.287199999993, "isoelectric_point": 5.539209938049315 }, "id": "EFPKPENHBP_926", "locus": "GCA000186005_00898" }, { "type": "cds", "sequence": "contig_1", "start": 501124, "stop": 501450, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVRKHYHYYFDMKLDNYYEQFINDFALKGTSKTFKDIAIDGLYKQGMWNNYFDLGKDYSKWFYLAFTRSNVEKYHEGFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "46c6c097a53fd654c69c00bd8f34eab2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13261.713499999989, "isoelectric_point": 5.872456932067871 }, "id": "EFPKPENHBP_927", "locus": "GCA000186005_00899" }, { "type": "cds", "sequence": "contig_1", "start": 501451, "stop": 501714, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGSQKDVFAYKDSYHGNK", "aa_hexdigest": "ea19c6d5ae4af4eb3676eadbf877fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.355799999998, "isoelectric_point": 4.955473518371581 }, "id": "EFPKPENHBP_928", "locus": "GCA000186005_00900" }, { "type": "cds", "sequence": "contig_1", "start": 501730, "stop": 502035, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MKKSKKFMKFMAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKYDADVYRNDN", "aa_hexdigest": "5ae4a8cbd911d43c80787cf35874157b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11541.700899999993, "isoelectric_point": 5.83141918182373 }, "id": "EFPKPENHBP_929", "locus": "GCA000186005_00901" }, { "type": "cds", "sequence": "contig_1", "start": 502116, "stop": 502313, "strand": "-", "frame": 2, "gene": null, "product": "ICEF-IA ORF13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945", "UniRef:UniRef90_Q8G945" ], "nt": "ATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGA", "aa": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVY", "aa_hexdigest": "37a7ed9202ae80cc53604b2722443644", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 502059, "stop": 502313, "observations": { "mutation": [ 502094, 502118 ], "stop": [ 502094, 502118 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8G945", "query_cov": 1.0, "subject_cov": 0.7738095238095238, "identity": 1.0, "score": 122.0, "evalue": 3.68e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVYWVKKRIEYWKQSKKNSDLH" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.29e-53, "description": "Internal stop codon at 502094, 502118. Nonsense mutation around 502094, 502118.", "psc": { "uniref90_id": "UniRef90_Q8G945", "product": "ICEF-IA ORF13", "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8G945", "UniRef:UniRef50_Q8G945" ] }, "pscc": { "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945" ], "product": "ICEF-IA ORF13" } }, "id": "EFPKPENHBP_930", "locus": "GCA000186005_00902" }, { "type": "cds", "sequence": "contig_1", "start": 502313, "stop": 502864, "strand": "-", "frame": 3, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "RefSeq:WP_013526867.1", "SO:0001217", "UniParc:UPI00000C02C3", "UniRef:UniRef100_C4XFA2", "UniRef:UniRef50_C4XFA2", "UniRef:UniRef90_C4XFA2" ], "nt": "ATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAA", "aa": "MNKVILTGRVVSEDYFKAKTTSGKAVLGFVIAVNNEYGGEPDFLNITAFGLQASNFEKYNKKGDLVEVIGKIKRNAYVDRDNHKQYKMEIVADNIIYSAKKVDRNAEGNDEDIIYKDETKLPKEEKEKVVKARLKKKDEMMEPFKQYIGMDPLCQSVYDKYEEEAFNKDVEVKDETKKHGQTL", "aa_hexdigest": "7876c7aa53c01bbcd9e8e8b7b6e1a631", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02C3", "ncbi_nrp_id": "WP_013526867.1", "uniref100_id": "UniRef100_C4XFA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C3", "RefSeq:WP_013526867.1", "UniRef:UniRef100_C4XFA2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA2", "uniref90_id": "UniRef90_C4XFA2" }, "psc": { "uniref90_id": "UniRef90_C4XFA2", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_C4XFA2" }, "pscc": { "uniref50_id": "UniRef50_C4XFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA2" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "EFPKPENHBP_931", "locus": "GCA000186005_00903" }, { "type": "cds", "sequence": "contig_1", "start": 502874, "stop": 503590, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0392 family ICE element protein", "db_xrefs": [ "RefSeq:WP_013526868.1", "SO:0001217", "UniParc:UPI00000C0686", "UniRef:UniRef100_C4XFA1", "UniRef:UniRef50_C4XFA1", "UniRef:UniRef90_C4XFA1" ], "nt": "ATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAA", "aa": "MIEKVFCFKDLYYLTQYALSDISDEEVVAKFNYLAKNIKNNTMRDKYKKQVLSIVDKMSNVDDDINQKIFDKLFYVEDTNEKNLEQYITLLNICNVEDLENIENNSLDLKFSFNDFFGENGYIESEYLFDLVDEDLKQNGINSKYIYSLGDLSRIDTEYVYIDDYLNFHPEDIDDIKKEYIENLASLKLDEIIDLNNKESIDANNKVEITDKTVKEAQKHVAGIVEKESETAKVKNTK", "aa_hexdigest": "7a9c47a621909a378797adedb1d43586", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C0686", "ncbi_nrp_id": "WP_013526868.1", "uniref100_id": "UniRef100_C4XFA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0686", "RefSeq:WP_013526868.1", "UniRef:UniRef100_C4XFA1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA1", "uniref90_id": "UniRef90_C4XFA1" }, "psc": { "uniref90_id": "UniRef90_C4XFA1", "product": "Mbov_0392 family ICE element protein", "uniref50_id": "UniRef50_C4XFA1" }, "pscc": { "uniref50_id": "UniRef50_C4XFA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA1" ] }, "genes": [], "id": "EFPKPENHBP_932", "locus": "GCA000186005_00904" }, { "type": "cds", "sequence": "contig_1", "start": 503603, "stop": 503842, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526869.1", "SO:0001217", "UniParc:UPI00000C02C2", "UniRef:UniRef100_C4XFA0", "UniRef:UniRef50_C4XFA0", "UniRef:UniRef90_C4XFA0" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEEFKKMEPEKYEKFRKESNKKICNKLFAK", "aa_hexdigest": "9df3ce0167d8cced414b46ed0b0c03a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02C2", "ncbi_nrp_id": "WP_013526869.1", "uniref100_id": "UniRef100_C4XFA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C2", "RefSeq:WP_013526869.1", "UniRef:UniRef100_C4XFA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA0", "uniref90_id": "UniRef90_C4XFA0" }, "psc": { "uniref90_id": "UniRef90_C4XFA0", "uniref50_id": "UniRef50_C4XFA0" }, "pscc": { "uniref50_id": "UniRef50_C4XFA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9481.049399999993, "isoelectric_point": 9.631012535095213 }, "id": "EFPKPENHBP_933", "locus": "GCA000186005_00905" }, { "type": "cds", "sequence": "contig_1", "start": 503851, "stop": 504798, "strand": "-", "frame": 1, "gene": null, "product": "MORN repeat protein", "db_xrefs": [ "RefSeq:WP_013526824.1", "SO:0001217", "UniParc:UPI00000C02A4", "UniRef:UniRef100_C4XF99", "UniRef:UniRef50_C4XF99", "UniRef:UniRef90_C4XF99" ], "nt": "ATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCTAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MQVFNALENEEIINRRNYEKNGTNFELISTKSGLTLAHNVLNDNSQEYYEMNEGAFSGRYYKIAENNQLVGVANYQNDELNGEATIYNTKGDILYKAHFEDGLLNGAVEEYKDGALVKSTMYENGFKTGEEVVYDAKGEVEGKTNFITNNEVKEKEVKEYKEADKEKKAEIRNKNFKEVLKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "0cdba05f663e8efc85d8182828c25163", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A4", "ncbi_nrp_id": "WP_013526824.1", "uniref100_id": "UniRef100_C4XF99", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A4", "RefSeq:WP_013526824.1", "UniRef:UniRef100_C4XF99" ] }, "ips": { "uniref100_id": "UniRef100_C4XF99", "uniref90_id": "UniRef90_C4XF99" }, "psc": { "uniref90_id": "UniRef90_C4XF99", "product": "MORN repeat protein", "uniref50_id": "UniRef50_C4XF99" }, "pscc": { "uniref50_id": "UniRef50_C4XF99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF99" ], "product": "MORN repeat protein" }, "genes": [], "id": "EFPKPENHBP_934", "locus": "GCA000186005_00906" }, { "type": "cds", "sequence": "contig_1", "start": 504800, "stop": 504988, "strand": "-", "frame": 3, "gene": null, "product": "ICEF-IA ORF6", "db_xrefs": [ "RefSeq:WP_013526825.1", "SO:0001217", "UniParc:UPI00000C073C", "UniRef:UniRef100_Q8G8G5", "UniRef:UniRef50_Q8G8G5", "UniRef:UniRef90_Q8G8G5" ], "nt": "ATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAA", "aa": "MKRYFYRIRVKKNLESKKTKSYAVCSNFNEADKIANEIIEKGDYGWVQVERVEKIKHYFKEV", "aa_hexdigest": "536d7d5d695db8bc2e435e625cfadd0d", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00000C073C", "ncbi_nrp_id": "WP_013526825.1", "uniref100_id": "UniRef100_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C073C", "RefSeq:WP_013526825.1", "UniRef:UniRef100_Q8G8G5" ] }, "ips": { "uniref100_id": "UniRef100_Q8G8G5", "uniref90_id": "UniRef90_Q8G8G5" }, "psc": { "uniref90_id": "UniRef90_Q8G8G5", "product": "ICEF-IA ORF6", "uniref50_id": "UniRef50_Q8G8G5" }, "pscc": { "uniref50_id": "UniRef50_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G8G5" ], "product": "ICEF-IA ORF6" }, "genes": [], "id": "EFPKPENHBP_935", "locus": "GCA000186005_00907" }, { "type": "cds", "sequence": "contig_1", "start": 505131, "stop": 505358, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGA", "aa": "MPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALA", "aa_hexdigest": "a7bce82dcd65675848f6e9422130e73d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8724.933399999994, "isoelectric_point": 9.221702766418456 }, "id": "EFPKPENHBP_936", "locus": "GCA000186005_00908" }, { "type": "cds", "sequence": "contig_1", "start": 505371, "stop": 505955, "strand": "-", "frame": 2, "gene": "traD", "product": "TraM recognition site of TraD and TraG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1", "UniRef:UniRef90_A0AB32XBH1" ], "nt": "ATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAGCTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGA", "aa": "MLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSANGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNES", "aa_hexdigest": "f918f8a0bf6604f81688e687aaff7c58", "start_type": "ATG", "rbs_motif": null, "genes": [ "traD" ], "truncated": "both", "pseudogene": { "start": 505068, "stop": 506237, "observations": { "mutation": [ 505133, 505373, 506192 ], "stop": [ 505133, 505373, 506192 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBH1", "query_cov": 1.0, "subject_cov": 0.470873786407767, "identity": 1.0, "score": 343.0, "evalue": 1.9e-115, "gene_start": 1, "gene_end": 194, "reference_start": 95, "reference_end": 288, "reference_sequence": "MSICYINAETNKRYYWAELFKNVKTIANIVPETLTGNLSHTTSALKNFTEDEFIKRLTRETKSFDLHGIVSSNEPYVIFIHYPDHKPSNHFLVSMLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNESWNYQDLPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALAWQKILSFQQKYKNKLSSELVNDFTKSINICLDQMNDIKTAGAKT" }, "paralog": false, "identity": 0.9897172236503856, "score": 728.0, "evalue": 1.37e-269, "description": "Internal stop codon at 505133, 505373, 506192. Nonsense mutation around 505133, 505373, 506192.", "psc": { "uniref90_id": "UniRef90_A0AB32XBH1", "product": "TraM recognition site of TraD and TraG", "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBH1", "UniRef:UniRef50_A0AB32XBH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1" ] } }, "id": "EFPKPENHBP_937", "locus": "GCA000186005_00909" }, { "type": "cds", "sequence": "contig_1", "start": 506256, "stop": 506396, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTGA", "aa": "MWVDNAKLIVNVVAQFLLLLSIDRPDLLPERHFNLQSVASMCNLDT", "aa_hexdigest": "1994a274baad84226fc2088274757087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5254.1116, "isoelectric_point": 4.74869327545166 }, "id": "EFPKPENHBP_938", "locus": "GCA000186005_00910" }, { "type": "cds", "sequence": "contig_1", "start": 506481, "stop": 506783, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGA", "aa": "MNEPESDINSVVLGGVGSGKTQRVLLPNIIYNSHLRYKYRANFCIPDPKKEIIKMVGKKLEKQGYKIYAVDFSDTKKSVGWNPLSFAYKLAHRVPEGHPK", "aa_hexdigest": "6390e01b408d03e3933cb69dff4b3dd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11349.04659999999, "isoelectric_point": 9.68065319061279 }, "id": "EFPKPENHBP_939", "locus": "GCA000186005_00911" }, { "type": "cds", "sequence": "contig_1", "start": 506922, "stop": 507218, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTTCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGA", "aa": "MGDKKKKILKISLSAVAILLAAIIIFLALAIIFNVKRLKSFKMVSIKDFFAQTKASIYAFPKQFLISYAFLLIASIAGISYLFYKNKLKTSLLKPNKY", "aa_hexdigest": "a7ee8fb1bcb6146dfc81f181ec136612", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11069.498099999988, "isoelectric_point": 10.299291801452636 }, "id": "EFPKPENHBP_940", "locus": "GCA000186005_00912" }, { "type": "cds", "sequence": "contig_1", "start": 507218, "stop": 507439, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAA", "aa": "MVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ", "aa_hexdigest": "4e375f5225f369e001680770108e444f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 507218, "stop": 507688, "observations": { "mutation": [ 507475 ], "stop": [ 507475 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.46794871794871795, "identity": 1.0, "score": 147.0, "evalue": 3.96e-44, "gene_start": 1, "gene_end": 73, "reference_start": 84, "reference_end": 156, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 8.41e-107, "description": "Internal stop codon at 507475. Nonsense mutation around 507475.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8364.290099999997, "isoelectric_point": 8.664953804016115 }, "id": "EFPKPENHBP_941", "locus": "GCA000186005_00913" }, { "type": "cds", "sequence": "contig_1", "start": 507473, "stop": 507685, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGGAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAACAATATGCCATTAAGAAACGTAGCACTTGTATTGTTTTCAATATCAATTTTAGTTTTCTTCATATTTGCTAGCCTAGTGAATGTATTAAATATAAGTTTTGCTTGA", "aa": "MGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFA", "aa_hexdigest": "0635f0269fe1de7e3229df2928cd1cab", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 507218, "stop": 507688, "observations": { "mutation": [ 507475 ], "stop": [ 507475 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.44871794871794873, "identity": 1.0, "score": 131.0, "evalue": 1.21e-37, "gene_start": 1, "gene_end": 70, "reference_start": 2, "reference_end": 71, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 7.52e-107, "description": "Internal stop codon at 507475. Nonsense mutation around 507475.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7962.459199999995, "isoelectric_point": 9.601679420471193 }, "id": "EFPKPENHBP_942", "locus": "GCA000186005_00914" }, { "type": "cds", "sequence": "contig_1", "start": 507779, "stop": 508120, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENMFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ca03355bf950c1fafc7488b9f7c761da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13355.14579999998, "isoelectric_point": 9.442893791198731 }, "id": "EFPKPENHBP_943", "locus": "GCA000186005_00915" }, { "type": "cds", "sequence": "contig_1", "start": 508226, "stop": 508366, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "EFPKPENHBP_944", "locus": "GCA000186005_00916" }, { "type": "cds", "sequence": "contig_1", "start": 508484, "stop": 508657, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGNLGLEHNVLTAND", "aa_hexdigest": "2e7232a416963aea6061be918ee46d1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.762199999999, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_945", "locus": "GCA000186005_00917" }, { "type": "cds", "sequence": "contig_1", "start": 508918, "stop": 509109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526872.1", "SO:0001217", "UniParc:UPI00000C0690", "UniRef:UniRef100_C4XF95", "UniRef:UniRef50_C4XF95", "UniRef:UniRef90_C4XF95" ], "nt": "ATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAG", "aa": "MKYKEGQLILVNYKFAQKFLSSSVGDDFLYLGLQNRKNLAKRLSQIMNIPKLTPEFHCIAKGS", "aa_hexdigest": "4e10fe405d634def018d3530d31e48fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C0690", "ncbi_nrp_id": "WP_013526872.1", "uniref100_id": "UniRef100_C4XF95", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0690", "RefSeq:WP_013526872.1", "UniRef:UniRef100_C4XF95" ] }, "ips": { "uniref100_id": "UniRef100_C4XF95", "uniref90_id": "UniRef90_C4XF95" }, "psc": { "uniref90_id": "UniRef90_C4XF95", "uniref50_id": "UniRef50_C4XF95" }, "pscc": { "uniref50_id": "UniRef50_C4XF95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF95" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.454599999999, "isoelectric_point": 9.779418754577634 }, "id": "EFPKPENHBP_946", "locus": "GCA000186005_00918" }, { "type": "cds", "sequence": "contig_1", "start": 509589, "stop": 510059, "strand": "-", "frame": 2, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANL", "aa_hexdigest": "adc8cbbec5672ec1b725519e74a278c1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 509340, "stop": 510122, "observations": { "mutation": [ 509570, 509591 ], "stop": [ 509570, 509591 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 1.0, "score": 304.0, "evalue": 6.56e-103, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9923076923076923, "score": 501.0, "evalue": 6.38e-183, "description": "Internal stop codon at 509570, 509591. Nonsense mutation around 509570, 509591.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "EFPKPENHBP_947", "locus": "GCA000186005_00919" }, { "type": "cds", "sequence": "contig_1", "start": 510224, "stop": 510478, "strand": "-", "frame": 3, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ2", "UniRef:UniRef90_C4XG76" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLEVIERSVLNTFFISLLIIYKSTHKH", "aa_hexdigest": "69c351ec48c5e3020f31cfdaa84e8132", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 510224, "stop": 510688, "observations": { "mutation": [ 510583, 510661 ], "stop": [ 510583, 510661 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG76", "query_cov": 1.0, "subject_cov": 0.5454545454545454, "identity": 1.0, "score": 162.0, "evalue": 9.74e-50, "gene_start": 1, "gene_end": 84, "reference_start": 71, "reference_end": 154, "reference_sequence": "MVDRKRKLEWTPNSGLFGKGFIFMQFKFKKVKRNKWNRDIKGYLKLKLDQKIKIIELYFQEFSILEISKIMENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLEVIERSVLNTFFISLLIIYKSTHKH" }, "paralog": false, "identity": 0.9805194805194806, "score": 296.0, "evalue": 5.56e-105, "description": "Internal stop codon at 510583, 510661. Nonsense mutation around 510583, 510661.", "psc": { "uniref90_id": "UniRef90_C4XG76", "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_C4XFJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG76", "UniRef:UniRef50_C4XFJ2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ2" ], "product": "Transposase" } }, "id": "EFPKPENHBP_948", "locus": "GCA000186005_00920" }, { "type": "cds", "sequence": "contig_1", "start": 510780, "stop": 511055, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY", "aa_hexdigest": "25f9e36794dd6b75a6d134d610038f68", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8053097345132744, "identity": 0.9890000000000001, "score": 174.0, "evalue": 4.75e-55, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10689.122099999988, "isoelectric_point": 7.929176139831544 }, "id": "EFPKPENHBP_949", "locus": "GCA000186005_00921" }, { "type": "cds", "sequence": "contig_1", "start": 511251, "stop": 511997, "strand": "-", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATTAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "c3fcc899803f7ec5ead3347237f6e376", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 510948, "stop": 512072, "observations": { "mutation": [ 511109, 511253 ], "stop": [ 511109, 511253 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 1.0, "score": 484.0, "evalue": 3.89e-170, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9919786096256684, "score": 742.0, "evalue": 5.33e-274, "description": "Internal stop codon at 511109, 511253. Nonsense mutation around 511109, 511253.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "EFPKPENHBP_950", "locus": "GCA000186005_00922" }, { "type": "cds", "sequence": "contig_1", "start": 512100, "stop": 512297, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTCAATTAAAAGAAGAATCGAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAATCTAAAAACAAGAAAAAATTACTTTCATGGCTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGA", "aa": "MMIQLKEESNLISGDKEFKLINIFTEEMLKEALEIKSKNKKKLLSWLIPVIILSVAGIIATVIAI", "aa_hexdigest": "f14970bb7a2b7d51169f76b839fd9002", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7341.860199999997, "isoelectric_point": 8.009826087951662 }, "id": "EFPKPENHBP_951", "locus": "GCA000186005_00923" }, { "type": "cds", "sequence": "contig_1", "start": 512337, "stop": 512774, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGGCTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAGCTTGAAATTGATAAGTTATATACTATTGATTTTACTGATTCATTTTTCAATAATTTAGATTTGTCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAATCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGATTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGA", "aa": "MENKKDPVKMAQIKNEAIRQVREKIIEEFKKRAPKLEIDKLYTIDFTDSFFNNLDLSIGTTLTLKGKHFMLKNPYTVNVINTHSSANKKLNLKDLNFEVITLSEKNLNDFKKALNEKIIDILKKENYPAKEIIKLIEFKLLENQK", "aa_hexdigest": "68bbb28bf58d56b7fd3ef29e1a1a764c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17010.710999999992, "isoelectric_point": 9.435415458679199 }, "id": "EFPKPENHBP_952", "locus": "GCA000186005_00924" }, { "type": "cds", "sequence": "contig_1", "start": 512925, "stop": 513335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATCTGCTAAAGATATAAATAACAAAGACATTGACTTGAATATTATCGAATTAAAACTTTTGAAAGATAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGACAAAAACTATTATATAGCTAATAATGATACATATACATACAATGTAAGTTTACTTAAATCAGATTATAGTTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATCAAAAAATACATTAAAGAACTATTAAAGAATTATTTAATCAAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATCACAATAGATGATATTAATATATCGTTCAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGACGATGACATTTATTGA", "aa": "MIKSAKDINNKDIDLNIIELKLLKDSDENQIIVKAISKDKNYYIANNDTYTYNVSLLKSDYSYLLNFNPGTLNVFGLKDKEEIKKYIKELLKNYLIKYNEKIDQSLYKKITIDDINISFNDEELNKIINPKDDDIY", "aa_hexdigest": "8a65a77a14df229a3af7d43c9dcf2f65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16028.07889999999, "isoelectric_point": 5.063240242004394 }, "id": "EFPKPENHBP_953", "locus": "GCA000186005_00925" }, { "type": "cds", "sequence": "contig_1", "start": 513676, "stop": 513912, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTATCAAGATCTTTTTTTGTTATGTTTAAATCAATAATTTTTTTCTTAGCTAAGTATTTTTCGAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTCTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAA", "aa": "MLISGLISKFSSKDFSIKKLLYSLKISSYFNLSRSFFVMFKSIIFFLAKYFSKSFETLRFLFLQNFLINDGEFASSVK", "aa_hexdigest": "155e05d570100d73304fda214788c006", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9153.74259999999, "isoelectric_point": 9.997321891784669 }, "id": "EFPKPENHBP_954", "locus": "GCA000186005_00926" }, { "type": "cds", "sequence": "contig_1", "start": 513948, "stop": 514064, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATATCTAATTCAGCTCCTGGGAAAGAAATCAAATTAACTAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGA", "aa": "MEISNSAPGKEIKLTKNGFYLIYDISLDKTNINKFKIV", "aa_hexdigest": "4e8472f64594401e7d09d6fa39250277", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4347.0371000000005, "isoelectric_point": 9.158330345153807 }, "id": "EFPKPENHBP_955", "locus": "GCA000186005_00927" }, { "type": "cds", "sequence": "contig_1", "start": 514089, "stop": 514451, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGCCCATATCCCTACGATGGATATTATTTTGGTAAAAATGTTCCTGATGAAGAAATAGAAAAAAATATCGAAATTCTATATGGGCTAATAGATTTATATGACAAAATAAATAAAATTGACTTTCTTCAAGACGAATCAGTAAACAAAGATAGTTATTCAAATTTAAAACAATTAAGAAAATATAAAAATCATTTTGATTCAAACTTTGCTTCAAATCCTTTTGGTTCATTTTATCTAAAATATAAAAATAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTAACTATTAATGCAAATTCAAATATTTTAATAAGAGAAATAAAACAAAATTCAATAGGAAAATGA", "aa": "MFGPYPYDGYYFGKNVPDEEIEKNIEILYGLIDLYDKINKIDFLQDESVNKDSYSNLKQLRKYKNHFDSNFASNPFGSFYLKYKNNNSSVLAEAIKTQYLTINANSNILIREIKQNSIGK", "aa_hexdigest": "d36c5471216c6bac02d91de7b169b522", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14013.55789999998, "isoelectric_point": 6.572372245788575 }, "id": "EFPKPENHBP_956", "locus": "GCA000186005_00928" }, { "type": "cds", "sequence": "contig_1", "start": 515841, "stop": 515951, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGA", "aa": "MKKLKNFLLGISSTIPASGVFLISSTNSKNEDSFNN", "aa_hexdigest": "a0e4b77d48cb83e2dc872eacbda44321", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3903.415100000001, "isoelectric_point": 9.524639701843263 }, "id": "EFPKPENHBP_957", "locus": "GCA000186005_00929" }, { "type": "cds", "sequence": "contig_1", "start": 515993, "stop": 516094, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAA", "aa": "MFSIDNKTRFQISIYYNQFEKDMIYKSGDLSNI", "aa_hexdigest": "035e85ec4bfd28e346bfa357462f8ecf", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.5033000000003, "isoelectric_point": 5.9190080642700185 }, "id": "EFPKPENHBP_958", "locus": "GCA000186005_00930" }, { "type": "cds", "sequence": "contig_1", "start": 516134, "stop": 516967, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGA", "aa": "MGKGLETKINPLQINYEIEKDNIKVSDELLISNHTKMVKEFFSLLYPDLDARDLRVANFLLNEFYLSNKIKNIQKLKNTDFPTMKDYIKFLKSYKFKDKKAELYYKDSLNKLSIIFDQDFNNNGIFSNLYNGHTNINFNKTLTVIDTYNLINNIASNEGQASLYLILSYVRNRISNNFFENKTRKICLIVDEAHRFINANNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSDEVMNLTKGMVENIQYTLIFRQNQNGVNAISQMYKSKNELTN", "aa_hexdigest": "280b7bca572fc66e13b2387afc433124", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 49, "aa_cov": 0.16967509025270758, "hmm_cov": 1.0, "evalue": 4.151631598292585e-06, "score": 22.68251609802246, "start": 185, "stop": 231 } ], "seq_stats": { "molecular_weight": 32361.543500000033, "isoelectric_point": 8.832313728332519 }, "id": "EFPKPENHBP_959", "locus": "GCA000186005_00931" }, { "type": "cds", "sequence": "contig_1", "start": 516974, "stop": 517105, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGA", "aa": "MPGSGKSATTSKIALHQFVVGNEVIIIDPQREYKDLCKKLDGN", "aa_hexdigest": "fc61c848a8aa12fc080e164cc7532130", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.368800000001, "isoelectric_point": 7.907514762878418 }, "id": "EFPKPENHBP_960", "locus": "GCA000186005_00932" }, { "type": "cds", "sequence": "contig_1", "start": 517241, "stop": 517576, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGA", "aa": "MSLNEANAETKSNFKMQKISVEQKVLDELVDFINVQKMNLFDSNIIFVNQSKTLEGIKDLETKNDINAKKAFANVYKMHYLQKQALQNSIFIKDDFLNNGIEMSSRNIVYG", "aa_hexdigest": "d468523640bb645f71595be49c8c4145", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12798.509799999985, "isoelectric_point": 6.572315406799317 }, "id": "EFPKPENHBP_961", "locus": "GCA000186005_00933" }, { "type": "cds", "sequence": "contig_1", "start": 517667, "stop": 518566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAACCTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTCAAAATGATTTTGAAAGTCTTAGAAATATTGAAAACATAGATGTCTATTATTTAGTTATCTATGCTAATGAAATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGA", "aa": "MAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKPSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYSQNDFESLRNIENIDVYYLVIYANEMNKLKQYENEFIKSFETTKIPLNVVTGMELIDFANMIFKKNISKSDKQNYFVEELDTELTKELDMKMQGQKLNDKVIKKLQSDKEYKLISLLAPERVEFNSKYIKVDNEYLSFQTLVDFNSLQINEG", "aa_hexdigest": "fe22ce43ff026985cdcb565578cfb4d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35192.32100000002, "isoelectric_point": 9.08876895904541 }, "id": "EFPKPENHBP_962", "locus": "GCA000186005_00934" }, { "type": "cds", "sequence": "contig_1", "start": 518725, "stop": 518862, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAA", "aa": "MDQLSKTLRGEKDLKKTSEISKELSELIENESSIKRIITSMERKK", "aa_hexdigest": "90459cbe5d27ff28c389422274ab21d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5252.027499999999, "isoelectric_point": 9.156267356872558 }, "id": "EFPKPENHBP_963", "locus": "GCA000186005_00935" }, { "type": "cds", "sequence": "contig_1", "start": 518893, "stop": 519354, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGA", "aa": "MLAIMILNLSISFMVLFFSGASKIIKPMSDATGIDSGICALIVYTGALITSLSLNKVMFGIFGVEYTSYLGKFTKKLAAKATKKISKRKSNSKNNSSSEPETTSQTDYHPTGNAKNAELIIIMQNNNQTKKPETTPQTIGAAKKAKKPETLND", "aa_hexdigest": "f013f8d8f6533028ea4dd7abb837224c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16557.087599999977, "isoelectric_point": 9.739512825012206 }, "id": "EFPKPENHBP_964", "locus": "GCA000186005_00936" }, { "type": "cds", "sequence": "contig_1", "start": 519376, "stop": 519579, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGA", "aa": "MKDELSLLNSNMGSLFLNSFIAIFALYIVGNITINISINVYQAFFLFIQAPLISVVSIEDGGKKLKV", "aa_hexdigest": "bcb457581ccf759ebf931c004962c8ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7397.688899999997, "isoelectric_point": 5.970674705505371 }, "id": "EFPKPENHBP_965", "locus": "GCA000186005_00937" }, { "type": "cds", "sequence": "contig_1", "start": 519733, "stop": 519969, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGA", "aa": "MIFGKPLSNINSYEDLKVLPGAFIGIAIFSICLFFIILAISAFKLSYNKSKESFQSFKASLKAIIPNLVFIIVIPILI", "aa_hexdigest": "c4a50977911cf7020c43ced63351ec3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8654.436699999991, "isoelectric_point": 9.519095420837402 }, "id": "EFPKPENHBP_966", "locus": "GCA000186005_00938" }, { "type": "cds", "sequence": "contig_1", "start": 520110, "stop": 520487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5", "UniRef:UniRef50_C4XEH2", "UniRef:UniRef90_C4XEH2" ], "nt": "TTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAA", "aa": "MIKSKKNTSTISDSAFFAFGKEKMKITSLYYANNNPGISVLKNTSTYVLDEFVKYSAIIFGILFAIAFVTSIITIAMNAAFTSKGNNTPAKLKKALNGSLISCGVLLALLILGPGLCGLISNSLK", "aa_hexdigest": "541bd4002d390de57c1f8e6291c84a4a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BE8", "uniref100_id": "UniRef100_A0AB32XAQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAQ5", "uniref90_id": "UniRef90_C4XEH2" }, "psc": { "uniref90_id": "UniRef90_C4XEH2", "uniref50_id": "UniRef50_C4XEH2" }, "pscc": { "uniref50_id": "UniRef50_C4XEH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13307.625799999976, "isoelectric_point": 9.769168281555174 }, "id": "EFPKPENHBP_967", "locus": "GCA000186005_00939" }, { "type": "cds", "sequence": "contig_1", "start": 520934, "stop": 521242, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGA", "aa": "MNSKQIQALDKIYLMMEEATRAFYRVKHFENLCKPYNTIGNQINLYVSMINLFKNLYNLKIDTNKTLKEQLQDLKPEVLRALEKSKNALNKLLPIIDSGFGS", "aa_hexdigest": "1cdeb99ea6952a6abf34c664964b4a43", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.784499999983, "isoelectric_point": 9.380423927307131 }, "id": "EFPKPENHBP_968", "locus": "GCA000186005_00940" }, { "type": "cds", "sequence": "contig_1", "start": 521438, "stop": 521596, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGA", "aa": "MKYFENKKFEVLDNKEEVEKNHKSPTYIYKNYIECVDALGYFMENYKDPDLK", "aa_hexdigest": "84e4dd2b6bf174ecff57cd3cc33d7ffb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6403.183200000001, "isoelectric_point": 5.264279747009277 }, "id": "EFPKPENHBP_969", "locus": "GCA000186005_00941" }, { "type": "cds", "sequence": "contig_1", "start": 521844, "stop": 522092, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAAATAACTTTTTTATCACTAAAACCTTCTGCATTTTCCTTTTTTATAGTTGGGTTTTTAGATGGTTTTGGTGGATTAATAGGAAAAGCAAACCAATTACAACTAGTTGCTGTTAAAGCAGTTACAATTGGTAATGTTATTGTTGGTAATATTGTCTTTAATCATATCTTTTTAGTTTTCATATTGCTCCTTATTTGCTATATAAATATTATACTTTATATTTATATTAAAATAAATCTTTAA", "aa": "MAEITFLSLKPSAFSFFIVGFLDGFGGLIGKANQLQLVAVKAVTIGNVIVGNIVFNHIFLVFILLLICYINIILYIYIKINL", "aa_hexdigest": "f50b57062a5a2a7a8f94084e8acefc52", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9064.948599999994, "isoelectric_point": 8.93223972320557 }, "id": "EFPKPENHBP_970", "locus": "GCA000186005_00942" }, { "type": "cds", "sequence": "contig_1", "start": 522440, "stop": 522580, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSKENNTIPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "cc5458cac4667834d74f24e26816b731", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5374.040500000001, "isoelectric_point": 8.099630546569824 }, "id": "EFPKPENHBP_971", "locus": "GCA000186005_00943" }, { "type": "cds", "sequence": "contig_1", "start": 522586, "stop": 522687, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 4.829350255693511e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "EFPKPENHBP_972", "locus": "GCA000186005_00944" }, { "type": "cds", "sequence": "contig_1", "start": 522730, "stop": 523176, "strand": "-", "frame": 1, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "4164854a7130acf16c96c160bbf5ab07", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 522586, "stop": 523215, "observations": { "mutation": [ 522732 ], "stop": [ 522732 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 9.07e-94, "description": "Internal stop codon at 522732. Nonsense mutation around 522732.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "EFPKPENHBP_973", "locus": "GCA000186005_00945" }, { "type": "cds", "sequence": "contig_1", "start": 523211, "stop": 523429, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "EFPKPENHBP_974", "locus": "GCA000186005_00946" }, { "type": "cds", "sequence": "contig_1", "start": 523703, "stop": 524062, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACATTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKHYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFRKNGDTSCLTVL", "aa_hexdigest": "34a89f6db1099ba4fc093a5902eb0beb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14369.686699999977, "isoelectric_point": 9.686068534851074 }, "id": "EFPKPENHBP_975", "locus": "GCA000186005_00947" }, { "type": "cds", "sequence": "contig_1", "start": 524082, "stop": 524417, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_C4XEG8" ], "nt": "TTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAA", "aa": "MFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA", "aa_hexdigest": "05d6c4774ad92cc4040ff46cab4c8f18", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 524082, "stop": 524579, "observations": { "mutation": [ 524420 ], "stop": [ 524420 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEG8", "query_cov": 1.0, "subject_cov": 0.6727272727272727, "identity": 0.991, "score": 216.0, "evalue": 2.87e-70, "gene_start": 1, "gene_end": 111, "reference_start": 55, "reference_end": 165, "reference_sequence": "MNNYKKQGENMNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDEWLFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA" }, "paralog": false, "identity": 0.9878787878787879, "score": 325.0, "evalue": 1.23e-115, "description": "Internal stop codon at 524420. Nonsense mutation around 524420.", "psc": { "uniref90_id": "UniRef90_C4XEG8", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEG8", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12876.668799999981, "isoelectric_point": 8.799241447448733 }, "id": "EFPKPENHBP_976", "locus": "GCA000186005_00948" }, { "type": "cds", "sequence": "contig_1", "start": 524418, "stop": 524549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGA", "aa": "MNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDE", "aa_hexdigest": "1bc3e1363e697e2abdb23ef609e7e097", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.7792, "isoelectric_point": 6.142328453063963 }, "id": "EFPKPENHBP_977", "locus": "GCA000186005_00949" }, { "type": "cds", "sequence": "contig_1", "start": 525070, "stop": 525765, "strand": "-", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGA", "aa": "MRKSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISMSTNYVKKYSEINKLTKPNVDIFDNLIKRLKIK", "aa_hexdigest": "9122dc5204b70e2613022a967e5f69c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "query_cov": 0.9653679653679653, "subject_cov": 1.0, "identity": 0.991, "score": 439.0, "evalue": 1.61e-155, "valid": true, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" }, "genes": [], "id": "EFPKPENHBP_978", "locus": "GCA000186005_00950" }, { "type": "cds", "sequence": "contig_1", "start": 526016, "stop": 526456, "strand": "-", "frame": 3, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLQPITSNNIVDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRFKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "f2a1161e604ea609c72117339484b6f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 0.993, "score": 279.0, "evalue": 5.88e-95, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "EFPKPENHBP_979", "locus": "GCA000186005_00951" }, { "type": "cds", "sequence": "contig_1", "start": 526475, "stop": 526657, "strand": "-", "frame": 3, "gene": "ruvA", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526656.1", "SO:0001217", "UniParc:UPI0001A8C12C", "UniRef:UniRef100_C4XEG5", "UniRef:UniRef50_C4XEG5", "UniRef:UniRef90_C4XEG5" ], "nt": "ATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAA", "aa": "MFENYVTTYKSSKEINETLKMLGFKDSLIDDVVRKINLNQDIEFSLEEAIKLMSDKPKTL", "aa_hexdigest": "c6bdaa9e4aa39c8bf37f7321449d566e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12C", "ncbi_nrp_id": "WP_013526656.1", "uniref100_id": "UniRef100_C4XEG5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12C", "RefSeq:WP_013526656.1", "UniRef:UniRef100_C4XEG5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG5", "uniref90_id": "UniRef90_C4XEG5" }, "psc": { "uniref90_id": "UniRef90_C4XEG5", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEG5" }, "pscc": { "uniref50_id": "UniRef50_C4XEG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG5" ], "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein" }, "genes": [ "ruvA" ], "id": "EFPKPENHBP_980", "locus": "GCA000186005_00952" }, { "type": "cds", "sequence": "contig_1", "start": 526769, "stop": 526960, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAATCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAAGTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAACACATTTTTAATTCTTGA", "aa": "MLPSLIKFIALSNPTFKTLNATLGPMPFKLSKSANNFLSSNSLKPYKDQVKSFLISYKHIFNS", "aa_hexdigest": "2f86f56e58c2d4a6a30a4f5d75ffa7b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.271900000002, "isoelectric_point": 10.176221656799317 }, "id": "EFPKPENHBP_981", "locus": "GCA000186005_00953" }, { "type": "cds", "sequence": "contig_1", "start": 526912, "stop": 527076, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGTATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESDNVGYYVTFPDNKSIKLNQELKMCLYEIKNDFT", "aa_hexdigest": "d4f2fe22c90eab35e78a6f8e6cf0c5a3", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6428.4094000000005, "isoelectric_point": 8.732967948913572 }, "id": "EFPKPENHBP_982", "locus": "GCA000186005_00954" }, { "type": "cds", "sequence": "contig_1", "start": 527090, "stop": 527560, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526745.1", "SO:0001217", "UniParc:UPI0001F5A115", "UniRef:UniRef100_C4XEG3", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAGTATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESISKLIDSKTIINFDIAKLQSINKYFGFGISDINLLKINQVLFDYELLKTLRLENNLLQLNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEEKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "915f0a1a2ff5d36ed2d11f97c42870a7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A115", "ncbi_nrp_id": "WP_013526745.1", "uniref100_id": "UniRef100_C4XEG3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A115", "RefSeq:WP_013526745.1", "UniRef:UniRef100_C4XEG3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG3", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18867.681699999994, "isoelectric_point": 8.92366542816162 }, "id": "EFPKPENHBP_983", "locus": "GCA000186005_00955" }, { "type": "cds", "sequence": "contig_1", "start": 528667, "stop": 529167, "strand": "-", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETELKRALTNDATSKLIKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYEKFIQYCAKRLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "db6b55899c8f181b6b7105e638679bda", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 528364, "stop": 529167, "observations": { "mutation": [ 528420, 528546, 528669 ], "stop": [ 528420, 528546, 528669 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9520000000000001, "score": 316.0, "evalue": 3.11e-107, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9550561797752809, "score": 510.0, "evalue": 3.67e-186, "description": "Internal stop codon at 528420, 528546, 528669. Nonsense mutation around 528420, 528546, 528669.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "EFPKPENHBP_984", "locus": "GCA000186005_00956" }, { "type": "cds", "sequence": "contig_1", "start": 530006, "stop": 530278, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTDNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "a6cf9241f23083862b43c93386ae60e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10698.441299999997, "isoelectric_point": 9.751826286315914 }, "id": "EFPKPENHBP_985", "locus": "GCA000186005_00957" }, { "type": "cds", "sequence": "contig_1", "start": 530390, "stop": 530614, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDELVSMNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "ee1a46cfc99fa70b8a20f44b791b3aad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8704.383199999993, "isoelectric_point": 10.111495399475096 }, "id": "EFPKPENHBP_986", "locus": "GCA000186005_00958" }, { "type": "cds", "sequence": "contig_1", "start": 530678, "stop": 530884, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 530575, "stop": 530884, "observations": { "insertion": [ 530605 ], "mutation": [ 530680 ], "stop": [ 530680 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9901960784313726, "score": 192.0, "evalue": 5.56e-65, "description": "Internal stop codon at 530680. Frameshift due to insertion around 530605. Nonsense mutation around 530680.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "EFPKPENHBP_987", "locus": "GCA000186005_00959" }, { "type": "cds", "sequence": "contig_1", "start": 531254, "stop": 531382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATGAGTTATACAATGTCATATTAACTTTAAGAAAAGAAATTAAAGAAAACTTTGAAAAAGATGTTCTAGTTATTGAAGAAGAACTTAAAAAAAGCGGTCATAAAAAATTAGCAGAAATATGA", "aa": "MIYELYNVILTLRKEIKENFEKDVLVIEEELKKSGHKKLAEI", "aa_hexdigest": "592dbaac8b237b3688e3fe44e99cd674", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5047.9042, "isoelectric_point": 5.739283180236817 }, "id": "EFPKPENHBP_988", "locus": "GCA000186005_00960" }, { "type": "cds", "sequence": "contig_1", "start": 531539, "stop": 531961, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTGAATATGACAATAATGTTTTGTATGACAAATCATTAGATATGACATTATCGAAAAATCAAGAAAACATAGCTCAAATATGTGACATGATGGATATGATAATTGGCAGCAGTCAAACCGGAATAATAAATAGTGATAATGTTGAATTAAATACTTTAATGAAAGAAGTTGAATCAGCAGTTAATTTTAAAGACAAATTGAGCTTTTTTACAAGTCAAAGTGGAAAAGACAAATTTTACCAATTCTTCGATAAAATGCTTTATTTAGCTTTAGATGTTAACGCTCAAATATTCAATGATTCAAGATTATTAGATGGTTTAACAGAAGACGATGAAAATCAACAACAAAGAAGTAGATTCTATATTCTTAAGCATAAATATTTTGAAAAAAATAAACAAATTTTAAATGAAGACTGA", "aa": "MSFEYDNNVLYDKSLDMTLSKNQENIAQICDMMDMIIGSSQTGIINSDNVELNTLMKEVESAVNFKDKLSFFTSQSGKDKFYQFFDKMLYLALDVNAQIFNDSRLLDGLTEDDENQQQRSRFYILKHKYFEKNKQILNED", "aa_hexdigest": "d439b38e85e9fa7d10c06e131121e0cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16448.27789999999, "isoelectric_point": 4.554019737243652 }, "id": "EFPKPENHBP_989", "locus": "GCA000186005_00961" }, { "type": "cds", "sequence": "contig_1", "start": 532879, "stop": 533517, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "ATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGA", "aa": "MLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTY", "aa_hexdigest": "62ccb3cb0c9a68c7c406150ba77312b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 218, "aa_cov": 0.9952830188679245, "hmm_cov": 1.0, "evalue": 1.1999266885350828e-71, "score": 237.5041961669922, "start": 1, "stop": 211 } ], "seq_stats": { "molecular_weight": 24290.117800000015, "isoelectric_point": 4.9036363601684565 }, "id": "EFPKPENHBP_990", "locus": "GCA000186005_00962" }, { "type": "cds", "sequence": "contig_1", "start": 533524, "stop": 534621, "strand": "+", "frame": 1, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281", "SO:0001217", "UniRef:UniRef50_A0A953T6S3", "UniRef:UniRef90_C4XEU9" ], "nt": "ATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAG", "aa": "MGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK", "aa_hexdigest": "37df700e8a13e5c038b373723d1a13b1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "5-prime", "pseudogene": { "start": 533224, "stop": 534621, "observations": { "mutation": [ 533515 ], "stop": [ 533515 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU9", "query_cov": 1.0, "subject_cov": 0.5496987951807228, "identity": 1.0, "score": 701.0, "evalue": 5.64e-250, "gene_start": 1, "gene_end": 365, "reference_start": 300, "reference_end": 664, "reference_sequence": "MRRAMNALDIKKKIIELSNKLNQWNKEYYQDNNPSVSDFEYDKALQELEQLEKKYPQFIYVNSPTQKLGSFADNKFKKVVHQKPMLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTYWNKMGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK" }, "paralog": false, "identity": 0.9978494623655914, "score": 905.0, "evalue": 0.0, "description": "Internal stop codon at 533515. Nonsense mutation around 533515.", "psc": { "uniref90_id": "UniRef90_C4XEU9", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0A953T6S3", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU9", "EC:6.5.1.2", "UniRef:UniRef50_A0A953T6S3", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0A953T6S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A953T6S3" ], "product": "DNA ligase" } }, "id": "EFPKPENHBP_991", "locus": "GCA000186005_00963" }, { "type": "cds", "sequence": "contig_1", "start": 534754, "stop": 535248, "strand": "+", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.1", "GO:0004807", "GO:0006096", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P50919", "UniRef:UniRef90_C4XEV0" ], "nt": "ATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGA", "aa": "MNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPI", "aa_hexdigest": "daac67b9ba546fb79c39dce817c0ffaf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 534682, "stop": 535470, "observations": { "mutation": [ 534748, 535246 ], "stop": [ 534748, 535246 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV0", "query_cov": 1.0, "subject_cov": 0.6259541984732825, "identity": 1.0, "score": 319.0, "evalue": 1.02e-108, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MYNLINYLGVIMDIRKYVVIGNWKMNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPIWAIGTGKVATPEIAQDVCQYIHSITSKELVVQYGGSVNPKNISDLAAQKDINGFLVGGASLNVDSFIKLITLGK" }, "paralog": false, "identity": 0.9885496183206107, "score": 509.0, "evalue": 3.74e-186, "description": "Internal stop codon at 534748, 535246. Nonsense mutation around 534748, 535246.", "psc": { "uniref90_id": "UniRef90_C4XEV0", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P50919", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV0", "EC:5.3.1.1", "UniRef:UniRef50_P50919", "KEGG:K01803", "GO:0004807", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P50919", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50919" ], "product": "Triosephosphate isomerase" } }, "id": "EFPKPENHBP_992", "locus": "GCA000186005_00964" }, { "type": "cds", "sequence": "contig_1", "start": 535561, "stop": 536298, "strand": "-", "frame": 1, "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "db_xrefs": [ "EC:3.5.99.6", "GO:0004342", "GO:0006044", "RefSeq:WP_013354622.1", "SO:0001217", "UniParc:UPI0001A8C230", "UniRef:UniRef100_C4XEV1", "UniRef:UniRef50_C4XEV1", "UniRef:UniRef90_C4XEV1" ], "nt": "ATGAAAGTATATATTGAAAAAGATGCTTTAGCAGTAGCACAAAAAGCATTAAGTTTAATTAAGAATCAAATTACTAAAAAAGCAGATTCAAGAATTTGCTTTGCCACAGGTTCAACACCTTTAGATTTATATAAATTATTAATTGAAGATTATCAAAAAGGTAAAATTGATTTTAGTAAAATGGTTACTTTTAACTTGGATGAATACCTTGGATTAGATTTAAAAAATGAACATTCTTATCATTATTTTATGGATCATAATTTGTTTAATCACATTAATGTTAAAAGAGAAAATATCAATTTTCCTGATGTCATAAAATATGAAAAAGAACATCAAAATGGTAGCTATGAACAAGTAATTGAAAAACTTGGAGGTATTGATTTTACTATTTTAGGTATTGGCGAAAACGGTCATATTGCATTTAATGAACCAGATAGTCAAGAACAAGATATTACAAGAGTAGTTGATTTAACTGAAAGCACAATTAATGCAAACAAAAGATTTTTTGCTAGTGAAAAAGATGTTCCTCGTAAAGCAGTAACTATGGGAATAGGAACTATTTTAAAAAGTAAAAAAATTATGTTATTAGCAACCGGGCCTAAAAAAGCTCAAGCAATTTATGATACTTTACTTAAACCAGTAAATTCAAATTGTCCAGCCAGCTTTTTACAAAATCATAAAGATGTAACAATTATTGTTGATGAATTATCAGCGAAATTAATCAAAAATAAAATTTAA", "aa": "MKVYIEKDALAVAQKALSLIKNQITKKADSRICFATGSTPLDLYKLLIEDYQKGKIDFSKMVTFNLDEYLGLDLKNEHSYHYFMDHNLFNHINVKRENINFPDVIKYEKEHQNGSYEQVIEKLGGIDFTILGIGENGHIAFNEPDSQEQDITRVVDLTESTINANKRFFASEKDVPRKAVTMGIGTILKSKKIMLLATGPKKAQAIYDTLLKPVNSNCPASFLQNHKDVTIIVDELSAKLIKNKI", "aa_hexdigest": "a3a0e82afc87760eaa544d701bc3c27e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C230", "ncbi_nrp_id": "WP_013354622.1", "uniref100_id": "UniRef100_C4XEV1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C230", "RefSeq:WP_013354622.1", "UniRef:UniRef100_C4XEV1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV1", "uniref90_id": "UniRef90_C4XEV1" }, "psc": { "uniref90_id": "UniRef90_C4XEV1", "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "ec_ids": [ "3.5.99.6" ], "uniref50_id": "UniRef50_C4XEV1", "go_ids": [ "GO:0004342", "GO:0006044" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEV1" ], "product": "Glucosamine-6-phosphate deaminase" }, "genes": [ "nagB" ], "id": "EFPKPENHBP_993", "locus": "GCA000186005_00965" }, { "type": "cds", "sequence": "contig_1", "start": 536311, "stop": 536796, "strand": "-", "frame": 1, "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "db_xrefs": [ "EC:3.5.1.25", "GO:0006044", "GO:0008448", "KEGG:K01443", "SO:0001217", "UniRef:UniRef50_L0RWM1", "UniRef:UniRef90_C4XEV2" ], "nt": "ATGAGTGGAATTGATTCAAGAAAACCGGGAATCTTAGAAGCTTCATTAGAAAGACATGATGTTTATAGTGAATTAATTGTTGATTTTCATCACATTTGTCAAGAAAGTATAGTTTTTACGCTTAATCATAAAGATATAAACCACTTAATGTGTGTTAGCGATGCAATTCGACCTGCTTATGGACCTGATGGTAAAAGCATTTCAGGTGGAATTAATATTGAAAAACATGGTTTAACAATTTATTTAGAGGGCACAACAACTATAGCTGGAAGTGGAATTTGCTTACATGATTCATTTAAAAATTTAGCTTCATTAGATTTAGATTATCGTGATATTGTTAAATTAACTTCATATAATGCAGCTAAAGCTCATAATTTAAAAGATCGAGCACAAATTAAAGCAAATAAATTAGCAGATTTAGTTTTAATGGATTTAAAAACTTTAGATATTAAAGAAGTTTATATTGATGGGCAAAAAGTCAAATAA", "aa": "MSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK", "aa_hexdigest": "c2794937e22f911759187dc2058a9756", "start_type": "ATG", "rbs_motif": null, "genes": [ "nagA" ], "truncated": "3-prime", "pseudogene": { "start": 536311, "stop": 537096, "observations": { "mutation": [ 536805, 537021 ], "stop": [ 536805, 537021 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV2", "query_cov": 1.0, "subject_cov": 0.4410958904109589, "identity": 1.0, "score": 323.0, "evalue": 8.47e-109, "gene_start": 1, "gene_end": 161, "reference_start": 205, "reference_end": 365, "reference_sequence": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTASWENIQIALKNIALAKCQGAKNIGIHIEGPFIGEAKKGAHKLEWLRRATREDILEMLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHLWNAMSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK" }, "paralog": false, "identity": 0.9923371647509579, "score": 517.0, "evalue": 1.28e-187, "description": "Internal stop codon at 536805, 537021. Nonsense mutation around 536805, 537021.", "psc": { "uniref90_id": "UniRef90_C4XEV2", "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "ec_ids": [ "3.5.1.25" ], "uniref50_id": "UniRef50_L0RWM1", "kegg_orthology_id": "K01443", "go_ids": [ "GO:0006044", "GO:0008448" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV2", "EC:3.5.1.25", "UniRef:UniRef50_L0RWM1", "KEGG:K01443", "GO:0006044", "GO:0008448" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWM1" ], "product": "N-acetylglucosamine-6-phosphate deacetylase" } }, "id": "EFPKPENHBP_994", "locus": "GCA000186005_00966" }, { "type": "cds", "sequence": "contig_1", "start": 536803, "stop": 536985, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTCAAATCATCAATTGAAAAAAATTAGTTATGATCCTTTAATGGTTGAAGAAAAATTAGTTCCTTTTATGGTTCAAAATGACATTATGCCTTCAATTGGTCACTCAGCTGCAAGCTATGATCAAGCTATGAAATACTATGATTTGGGTTCAACTAATACTTGTCACTTATGA", "aa": "MLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHL", "aa_hexdigest": "0a06cc54445ef5fe5a341ea80c828741", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6806.729700000002, "isoelectric_point": 5.756505393981934 }, "id": "EFPKPENHBP_995", "locus": "GCA000186005_00967" }, { "type": "cds", "sequence": "contig_1", "start": 537145, "stop": 537408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01979.26" ], "nt": "ATGCTAATAAAAAACGTTAAAATAGTTAATCACAACCAAATTATTGAGTGTGCTGACATTGAGTTTAGTAAAAAAATTACTAAAATAACTCCAACTAAAAATAAGGCTCAATATTTATTAGTTCCAGGCTTTATTGACACTCATATTCATGGTTTTATGAATAAGGATGTAATGGATAGTAGTGCAGCAGTTGAAGATATTAGTTTAAATTTAGCTAGAAATGGAATTACTAGTTTTATGCCTACAGCAATGACAGCAAGTTGA", "aa": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTAS", "aa_hexdigest": "6961c50969b1e300b5bdb9937c776592", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01979.26", "name": "Amidohydro_1", "length": 58, "aa_cov": 0.5287356321839081, "hmm_cov": 1.0, "evalue": 1.6118086645576435e-07, "score": 27.16958236694336, "start": 40, "stop": 85 } ], "seq_stats": { "molecular_weight": 9636.156699999994, "isoelectric_point": 7.90738582611084 }, "id": "EFPKPENHBP_996", "locus": "GCA000186005_00968" }, { "type": "cds", "sequence": "contig_1", "start": 537641, "stop": 538054, "strand": "+", "frame": 2, "gene": null, "product": "Phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "nt": "ATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGA", "aa": "MKKTIDDLVLKGKKVLMRVDFNVPLKDGVITSNKRIVAALPTIEKVIKEKGKLILFSHLGRVKSEEDKAKNNLLPVANELARLLKKPVLFVDSTRGAELEKAIAKMEPGEVILVQNTRYEDLNEKAESKNNPILGKY", "aa_hexdigest": "34e8d9ace610e7b2b32a6d05df889a28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378MFN7", "query_cov": 0.9343065693430657, "subject_cov": 0.8767123287671232, "identity": 0.617, "score": 151.0, "evalue": 1.64e-44, "valid": false, "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A378MFN7", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_A0A378MFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "product": "Phosphoglycerate kinase" }, "genes": [], "id": "EFPKPENHBP_997", "locus": "GCA000186005_00969" }, { "type": "cds", "sequence": "contig_1", "start": 538166, "stop": 538585, "strand": "+", "frame": 2, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "nt": "ATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGA", "aa": "MEKEVNALTKAIKNPKKPYVAIIGGAKVSDKIQVLENLIPLVDKMIIGGGMAYTFLKSQGYKIGKSLVEDEFIPFATEFLKKNKDKIILPVDHACAKEFADVEPVVYGLDIDDNYDGLDLGPKSIEKFVSALEGAKTVV", "aa_hexdigest": "c0d9705ec49c9e350b532b7ff52e19a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ACD8294", "query_cov": 0.8992805755395683, "subject_cov": 0.984251968503937, "identity": 0.536, "score": 124.0, "evalue": 4.22e-34, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI002ACD8294", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI002ACD8294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "EFPKPENHBP_998", "locus": "GCA000186005_00970" }, { "type": "cds", "sequence": "contig_1", "start": 538595, "stop": 538825, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTTCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAG", "aa": "MGVTEFENYKTGTLEVAKAVSALKGCYSIVGGGDSVAAVQKLKMESKFSHVSTGGGASLEFLQGIALPGVEAIQNK", "aa_hexdigest": "6362537120497bf39f886349fa8ef66e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002A7EA9FD", "query_cov": 1.0263157894736843, "subject_cov": 0.9176470588235294, "identity": 0.564, "score": 85.5, "evalue": 2.57e-20, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A166IH01", "kegg_orthology_id": "K00927" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 65, "aa_cov": 0.8421052631578947, "hmm_cov": 1.0, "evalue": 1.4513549090578132e-24, "score": 83.04300689697266, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7806.853399999999, "isoelectric_point": 6.544691658020019 }, "id": "EFPKPENHBP_999", "locus": "GCA000186005_00971" }, { "type": "cds", "sequence": "contig_1", "start": 538880, "stop": 539005, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCAAGATACAAAATTCATTACAGGAATTATGATTAGCACTAATAAAATTTCATTAGTTATTGGTGATTATGCACGTAAGTTAGCTCTTGAAATGAATGAATATTTAGAAAAGTAA", "aa": "MDKLQDTKFITGIMISTNKISLVIGDYARKLALEMNEYLEK", "aa_hexdigest": "9cc1c20f22f06a4ddd509858ca720f13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4735.540999999999, "isoelectric_point": 6.060423469543457 }, "id": "EFPKPENHBP_1000", "locus": "GCA000186005_00972" }, { "type": "cds", "sequence": "contig_1", "start": 539406, "stop": 539690, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGA", "aa": "MSKKRLLSGIKPTGDLTLGNYIGALRNFVKLQENYDEAYYFVADLHALTTGYVDPKELYQARREIVTMYLACGLDPKKSTIFYQSDVVEHTLAQ", "aa_hexdigest": "12e4e8eaa0cebc5bd1158ac7bc69e37f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10710.146499999997, "isoelectric_point": 6.712594032287597 }, "id": "EFPKPENHBP_1001", "locus": "GCA000186005_00973" }, { "type": "cds", "sequence": "contig_1", "start": 539694, "stop": 540407, "strand": "+", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAA", "aa": "MTTEISIGELSRMTQFKDKSQKMSKQANGTEKIPVGLLMYPVLMAADILIYNPDFVPIGEDQKQHLELARTIAERLNRDYKTNFKLAEGIIPPVGARIMSLTDPTKKMSKSDKGTKATIYLHDDPEVAYKKIMKSVTDSENKVYISEDKPGVLNLLNIYASLTNKTLKEAEKEFKDKDYAQFKSAVAIVVKDELTKIQANYAKATKMVDKVVAEGAQKAQAICQPIVEDLMKKMGYR", "aa_hexdigest": "6bb5c218c02ae2bd8eb195662435a1b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 0.9915611814345991, "subject_cov": 0.9791666666666666, "identity": 0.574, "score": 260.0, "evalue": 1.1e-84, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "EFPKPENHBP_1002", "locus": "GCA000186005_00974" }, { "type": "cds", "sequence": "contig_1", "start": 540671, "stop": 541105, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "ATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGA", "aa": "MKIQVDKLLNHTTSHLLGAAVEKLYPEVKLGFGPATEEGFYYDFEFKEPLSVNELSKIEKLMKKLASRNLVTIQIPEKDYDFKNKPYKKELYDELKAQGKKITFYALQDPLNKEIIFKDLCAGGHVESTKVIKNFKLLSIAGAY", "aa_hexdigest": "4a2b8493d6aab52ff09f2d16689b2883", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.2569444444444444, "hmm_cov": 0.8378378378378378, "evalue": 3.5312127747844055e-08, "score": 29.6651611328125, "start": 103, "stop": 139 } ], "seq_stats": { "molecular_weight": 16492.008699999984, "isoelectric_point": 8.765911293029784 }, "id": "EFPKPENHBP_1003", "locus": "GCA000186005_00975" }, { "type": "cds", "sequence": "contig_1", "start": 541301, "stop": 541807, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGA", "aa": "MYIHNEIKNLVLKMDRKYGFTEVLTPHFGSEDLYKTSGHLAHYKDDMFAPLVIENERLIPRPMTCPHHIVCYNLEKRSYRDLPIRYSEQSQLYRYEKSGALTGLERVRGMLLTEGHLFVREDQIEQEIKSMYQLIEETLKIFNIDISYISLSLRDKNDKEKYFNDNKM", "aa_hexdigest": "1733c70d9481a3e87c054679e4699825", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.6130952380952381, "hmm_cov": 0.9809523809523809, "evalue": 2.2783931059526567e-18, "score": 63.21187973022461, "start": 54, "stop": 156 } ], "seq_stats": { "molecular_weight": 20087.83030000001, "isoelectric_point": 6.385428810119628 }, "id": "EFPKPENHBP_1004", "locus": "GCA000186005_00976" }, { "type": "cds", "sequence": "contig_1", "start": 541823, "stop": 542113, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGA", "aa": "MLKKALDDMGVKYTTVVGEAAFYGPKIDIQIDTALGHEITVSTIQLDFLQPKNFDITYTDKNGKEARPVMIHRGLIGTYERFVAILLEQTKGNLPF", "aa_hexdigest": "1c0de043d776b6270796897da626c34f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8645833333333334, "subject_cov": 0.8217821782178217, "identity": 0.639, "score": 109.0, "evalue": 2.29e-29, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 89, "aa_cov": 0.8645833333333334, "hmm_cov": 0.9775280898876404, "evalue": 1.781283382082897e-15, "score": 53.78683853149414, "start": 7, "stop": 89 } ], "seq_stats": { "molecular_weight": 10774.359399999992, "isoelectric_point": 6.056899452209472 }, "id": "EFPKPENHBP_1005", "locus": "GCA000186005_00977" }, { "type": "cds", "sequence": "contig_1", "start": 542267, "stop": 542419, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAG", "aa": "MSKSKYQVILGANEEKDKTISYREYGKEDTKTVTVEEFINILTKLRNNYE", "aa_hexdigest": "1018cd1ce96b3544e9adc90a81fea955", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 42, "aa_cov": 0.84, "hmm_cov": 1.0, "evalue": 2.682872146536773e-06, "score": 23.8287296295166, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 5918.595899999999, "isoelectric_point": 6.2491857528686525 }, "id": "EFPKPENHBP_1006", "locus": "GCA000186005_00978" }, { "type": "cds", "sequence": "contig_1", "start": 542975, "stop": 543502, "strand": "+", "frame": 2, "gene": null, "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354629.1", "SO:0001217", "UniParc:UPI0001E330EE", "UniRef:UniRef100_C4XEV9", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_C4XEV9" ], "nt": "ATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAA", "aa": "MKKIICVIDMLEGFCNEGALASPIVKKIIPNIEKVLKDNKKEDNLFICDSHNTFDLEMKQYPLHCLKDTKEAEIVKELKPYVKSVLEKNSTNAFHLFDKKLIEKYDQFVLTGCCTDICVLQFALSLKTYLNENRIDKKVIVLKDAVATFDAPGHNAQQFHNFTLNLMANAGIEIQ", "aa_hexdigest": "d5053b3198c14a52349f1c515f70fa1e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330EE", "ncbi_nrp_id": "WP_013354629.1", "uniref100_id": "UniRef100_C4XEV9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EE", "RefSeq:WP_013354629.1", "UniRef:UniRef100_C4XEV9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV9", "uniref90_id": "UniRef90_C4XEV9" }, "psc": { "uniref90_id": "UniRef90_C4XEV9", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1007", "locus": "GCA000186005_00979" }, { "type": "cds", "sequence": "contig_1", "start": 543505, "stop": 543786, "strand": "+", "frame": 1, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354630.1", "SO:0001217", "UniParc:UPI0001E330EF", "UniRef:UniRef100_C4XEW0", "UniRef:UniRef50_C4XEW0", "UniRef:UniRef90_C4XEW0" ], "nt": "ATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAA", "aa": "MTKTIDLHKLNVEQATSKVILALSEAQEKKLTSLEIITGYGSGALRARTLELLESENLTYIEEGPMVIVYFFNNNEDENDDNFSDIEYQKKFQ", "aa_hexdigest": "e7b8c658c22a427049059c3588eff924", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330EF", "ncbi_nrp_id": "WP_013354630.1", "uniref100_id": "UniRef100_C4XEW0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EF", "RefSeq:WP_013354630.1", "UniRef:UniRef100_C4XEW0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEW0", "uniref90_id": "UniRef90_C4XEW0" }, "psc": { "uniref90_id": "UniRef90_C4XEW0", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEW0" }, "pscc": { "uniref50_id": "UniRef50_C4XEW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW0" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1008", "locus": "GCA000186005_00980" }, { "type": "cds", "sequence": "contig_1", "start": 543841, "stop": 544026, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGACTTTTATTTAATATACTTAGCTTTACAATTATTAGTTCCTTTTGGACTTGAAATATCAGTCAAAGAAGAAATTGTCAACAAGCCTTTAGAAAGTGATTTAACTTTTGAAGGTGAAAAAGAACAAAATAAAGATAATAATCAGGAAAATAATCAAACACAATCAAAAAATAGTAAATAG", "aa": "MDDFYLIYLALQLLVPFGLEISVKEEIVNKPLESDLTFEGEKEQNKDNNQENNQTQSKNSK", "aa_hexdigest": "6cba0285e07590f901d81f9ad8ff4fde", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7090.793200000002, "isoelectric_point": 4.339623069763184 }, "id": "EFPKPENHBP_1009", "locus": "GCA000186005_00981" }, { "type": "cds", "sequence": "contig_1", "start": 544067, "stop": 544261, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTGGCTTCAAAGATTAAATCAATTAATTCAAAAGGAATGTCAAATCAAATAAATGCTTCTGTATTTCTAATTAAGAATGATAAATTATTTAATAAATATTTTTTATTCGCTTTAGTGTTAGGAACACTAATTTGGCTAATAGTCAATTCATTGAAAATTTCATTTAAGAATTCTAAATTATTTTTTTGA", "aa": "MVLASKIKSINSKGMSNQINASVFLIKNDKLFNKYFLFALVLGTLIWLIVNSLKISFKNSKLFF", "aa_hexdigest": "2cb561f63287978b91bc3b3c5172e282", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.754299999998, "isoelectric_point": 10.368079566955565 }, "id": "EFPKPENHBP_1010", "locus": "GCA000186005_00982" }, { "type": "cds", "sequence": "contig_1", "start": 544534, "stop": 544884, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTTGGTATTTCATTAACTTTAACTATCCCTGGCTTAATTGTTCTTTTAATAGGACAAAAAGCTAAAAAATATGTGCTGAAAGATGTTATTACCAAATTTGATAAATATAAATTAATCAAAAAATTATTTAACTTCTTTACTCTTGGTTACAAAGAAAATATCGTTGATGAAAATAACAAAATTCACAAAAATTATGACTTAATCAATTTTTATAAAAACACACATAATTTCAAAAATTTTGAATTTGGTTTTGCTCCAATCCATAAAAAATATGAACAATATGAAGCTTTAACTGATAACCAATATTTAAAGATGCAAAATGTTTACTACGAAGGCAACATTTGA", "aa": "MLFGISLTLTIPGLIVLLIGQKAKKYVLKDVITKFDKYKLIKKLFNFFTLGYKENIVDENNKIHKNYDLINFYKNTHNFKNFEFGFAPIHKKYEQYEALTDNQYLKMQNVYYEGNI", "aa_hexdigest": "7fff1fc8bc39a78472e457ec01c4bc53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13861.003299999984, "isoelectric_point": 9.357150840759278 }, "id": "EFPKPENHBP_1011", "locus": "GCA000186005_00983" }, { "type": "cds", "sequence": "contig_1", "start": 545023, "stop": 545154, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTATCAAATAAGTTAATTTCAAAAGAAGCTTTTATTCGTTCTAAACGTCCAACAATAAACAAATTAACTACTCAATTAATTAATAATTATTATGCTGATAAAAAGAAAAGCAAAAGGATTAATTGA", "aa": "MSVSNKLISKEAFIRSKRPTINKLTTQLINNYYADKKKSKRIN", "aa_hexdigest": "69bbbeab83aa6042f17205e2270e75ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5041.871600000003, "isoelectric_point": 10.573346900939939 }, "id": "EFPKPENHBP_1012", "locus": "GCA000186005_00984" }, { "type": "cds", "sequence": "contig_1", "start": 545281, "stop": 545742, "strand": "-", "frame": 1, "gene": null, "product": "Deoxyribose-phosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "nt": "ATGCTTACAAGTGCTAAGGCATTTGAAGCTAAAGAAGCTATTAAAGCTGGTGCTGATGAAATTGACATGGTTATTCAAATTGGAAAAATGAAAATGGGTGACTATGACTATGTTTTAGAAGACATTAAAGCGGTTAAAAAAGCATGCGGCAAACATGTTTTAAAAGTTATTATTGAAACAGCTTTATTAACAGAAGCTGAAATTAGAAAAGCTACTGAAATCGTTATGGCTTCTGATGCTGAATTCATTAAAACTTCAACAGGTTTCTCAACAAGAGGCGCAACACTTGAAGATGTTAAAATCATGAAAAGTGTTTGTGGAGACAAATTATTAATCAAAGCTGCTGGTGGAATCAGCAACAAAGATGACATGATCAACATGGCTCAAGCTGGCGCAAACCGTTTCGGAACAAGCCGTTCAGTTGCCATTGTTGAAGGTAAATCTTCAAAAGAAGGGTACTAA", "aa": "MLTSAKAFEAKEAIKAGADEIDMVIQIGKMKMGDYDYVLEDIKAVKKACGKHVLKVIIETALLTEAEIRKATEIVMASDAEFIKTSTGFSTRGATLEDVKIMKSVCGDKLLIKAAGGISNKDDMINMAQAGANRFGTSRSVAIVEGKSSKEGY", "aa_hexdigest": "ea58885d93b8b8d4fe29a208fefe0b40", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00220D32D4", "query_cov": 0.9673202614379085, "subject_cov": 0.9367088607594937, "identity": 0.77, "score": 212.0, "evalue": 2.92e-68, "valid": false, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_A0A139MP28", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0009264" ] }, "pscc": { "uniref50_id": "UniRef50_A0A139MP28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [], "id": "EFPKPENHBP_1013", "locus": "GCA000186005_00985" }, { "type": "cds", "sequence": "contig_1", "start": 545818, "stop": 545949, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTAATTACAACAAAATGATTGATCACACTTATTTAAAAGCTGATGCGACAAATAAAGAAATTGATAATTTATTAGATGAAGCCAAGAAATATGACTTCAAAACTGTTTGTGTTAATTCATCATGA", "aa": "MQINYNKMIDHTYLKADATNKEIDNLLDEAKKYDFKTVCVNSS", "aa_hexdigest": "cec1bd5b04f178dbf5da8c6d6cd17892", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.631500000001, "isoelectric_point": 5.553703880310058 }, "id": "EFPKPENHBP_1014", "locus": "GCA000186005_00986" }, { "type": "cds", "sequence": "contig_1", "start": 545972, "stop": 546196, "strand": "-", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4", "UniRef:UniRef90_A0A3P8MDY4" ], "nt": "ATGAAACTTGGCGCCGGCCGTGCTAAAAAAGAAGATAGTATTGACTTTGAAGCAGGAATTACTCTTCACGCTAAAACAAATCATAAAGTAACTAAAGGGCAAAAACTATTTACTCTTTATTCTTCAAATCCGATTGACAAGAATTTAGTTAAAGAATTAGAAACAGGTTACAAAATTGGCAATTCAAAAGTTGAAAATAAGATTATAATTGCTAAGTTGAAATAA", "aa": "MKLGAGRAKKEDSIDFEAGITLHAKTNHKVTKGQKLFTLYSSNPIDKNLVKELETGYKIGNSKVENKIIIAKLK", "aa_hexdigest": "96115f3718663ff416c9a1c04620841f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.905, "score": 132.0, "evalue": 3.06e-39, "valid": true, "product": "Pyrimidine-nucleoside phosphorylase", "uniref50_id": "UniRef50_A0A3P8MDY4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "EFPKPENHBP_1015", "locus": "GCA000186005_00987" }, { "type": "cds", "sequence": "contig_1", "start": 546494, "stop": 547267, "strand": "-", "frame": 3, "gene": null, "product": "thymidine phosphorylase", "db_xrefs": [ "EC:2.4.2.4", "GO:0006206", "SO:0001217", "UniRef:UniRef50_R5VCS9", "UniRef:UniRef90_A0A3P8LBE3" ], "nt": "ATGCGTGTCGTTGATTTAATCGAAAAGAAAAGAGAAGGTAAAGAATTAACAACTCAAGAAATTGAATTCTTAATTACTTCTTATGTATCAGGTAATACACCTGATTACCAAATGGCTGCTTTTATAATGGCAGTTATGTTCCAAGGAATGGCAAAAAAAGAATTAGCAACTTTTACTAAAACCATGATGCACTCAGGTGAAGTTATTGATTTAAGTGAAATTCCAGGGATTAAAGTTGATAAACACTCAACAGGTGGAGTTGGAGATAAAACTACTTTAGCAGTTGCTCCTATTTGTGCCGCTTTAGGTGCTCCAGTAGCGAAAATGAGTGGTAGAGGTTTAGGCCACACTGGTGGAACAATTGATAAACTTGAATCTATTCCAGGATTTCATGTTGAATTAACTGAACGTCAATTTATTGAACAAGTTAAAAAACATAATATTTCAGTTATTGGTCAATCAAAAGAATTAGTACCAGCTGATAAAAAACTTTATGCTTTAAGAGATGTTACTGCTTGTGTTAACTCAATTCCTCTTATTGCTTCAAGTATTATGTCTAAAAAATTAGCTACAGGTTCAAATGCTATTTTATTAGATGTTAAATGCGGTAAAGGCGCCTTCATGAAAAATGAAGACCAAGCTAAAGAATTAGCTAAAACTATGATTTCAATTGGTAAAGAATTAAACGTTGATGTTCGTGCAGAAATTACCAACATGAACCGTCCAATTGGTTGTGAAATTGGAAACAAAAATGAAGTTTTAGAAGCTATTTGA", "aa": "MRVVDLIEKKREGKELTTQEIEFLITSYVSGNTPDYQMAAFIMAVMFQGMAKKELATFTKTMMHSGEVIDLSEIPGIKVDKHSTGGVGDKTTLAVAPICAALGAPVAKMSGRGLGHTGGTIDKLESIPGFHVELTERQFIEQVKKHNISVIGQSKELVPADKKLYALRDVTACVNSIPLIASSIMSKKLATGSNAILLDVKCGKGAFMKNEDQAKELAKTMISIGKELNVDVRAEITNMNRPIGCEIGNKNEVLEAI", "aa_hexdigest": "56c498535f478982cacbcac4e28cd371", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LBE3", "query_cov": 1.0, "subject_cov": 0.9178571428571428, "identity": 0.9179999999999999, "score": 458.0, "evalue": 8.64e-162, "valid": true, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_R5VCS9", "go_ids": [ "GO:0006206" ] }, "genes": [], "id": "EFPKPENHBP_1016", "locus": "GCA000186005_00988" }, { "type": "cds", "sequence": "contig_1", "start": 547281, "stop": 547985, "strand": "-", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_013526885.1", "SO:0001217", "UniParc:UPI0001A8C23D", "UniRef:UniRef100_C4XEW4", "UniRef:UniRef50_S5MIP4", "UniRef:UniRef90_C4XEW4" ], "nt": "ATGACACCACACATTAATGCCAAAGAAGGTGATATTGCTAAGACAGTTTTAATGCCAGGAGATCCTTTAAGAGCTAAATTTATTGCTGAAACATTTTTAGACAAAGGATATAGACTTGTTAATACTGTCAGAAACATGTACATGTATACAGGTACATATAAAGGTCGTCCAGTAACAATCGCTGGAAGTGGAATGGGTTGTCCATCAATTGGGATCTATTCATATGAATTATTCAAATTCTACAATGTAGATAACATTATTAGAATCGGTACAACTGGTTCATACAAAAAAGAAATCAAACTATATGAAACGATTTTAGCAACTGAAGCTTATGCAGATGGTGCTAGTTATCGTCGTTTAGTTTTAGGAGACAAAAGCCATGTAGCTTTACCTAGTGCTAAATTAAATAAAGAAATTGAAAAAGTGGCTAAAAATTTGAACATTAAATTAACTTTAGGCCGTGTACACTCTTCAGATGTATTCTATTCATCAGTTCCTCTTCAACAAAGAATCGATGAAACACATGCGCTTTGTGTTGAAATGGAATCATATGCTTTATTTACTAATGCTGAAAAATTAGGTAAAAATGCAGCATGTCTTTTAACAGTTAGTGACAACTTAATTACAAATGACGAAACAACACCCGAAGAAAGACAAACAGCTTTCAAAAACATGATGGAAATTGCTTTAGGTGTTGCAAAATAA", "aa": "MTPHINAKEGDIAKTVLMPGDPLRAKFIAETFLDKGYRLVNTVRNMYMYTGTYKGRPVTIAGSGMGCPSIGIYSYELFKFYNVDNIIRIGTTGSYKKEIKLYETILATEAYADGASYRRLVLGDKSHVALPSAKLNKEIEKVAKNLNIKLTLGRVHSSDVFYSSVPLQQRIDETHALCVEMESYALFTNAEKLGKNAACLLTVSDNLITNDETTPEERQTAFKNMMEIALGVAK", "aa_hexdigest": "86abc929af10e3782e99a3cae902b30d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C23D", "ncbi_nrp_id": "WP_013526885.1", "uniref100_id": "UniRef100_C4XEW4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C23D", "RefSeq:WP_013526885.1", "UniRef:UniRef100_C4XEW4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEW4", "uniref90_id": "UniRef90_C4XEW4" }, "psc": { "uniref90_id": "UniRef90_C4XEW4", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_S5MIP4", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_S5MIP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5MIP4" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "EFPKPENHBP_1017", "locus": "GCA000186005_00989" }, { "type": "cds", "sequence": "contig_1", "start": 548375, "stop": 549112, "strand": "+", "frame": 2, "gene": null, "product": "ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "nt": "ATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGA", "aa": "MKKIKRIAILTSGGDAPGMNSAIRAAAKMARSRGIETFLVYEGYKGLVDDNIVPSTKIDLDFFNSRGGTCIYSARYPQFKDPEIREIAIKNLNKRKIDALIVIGGDGSYAGAQLLHEKGIKTIGLPGTIDNDIASSDFTIGYDTALNTVVRCIDQIRDTAASHKRIMIIEVMGNNCGDLALHTGLATGAEVIATSEYRLDEKEIVKTCVELTTNAKPVRRNITIVVSEKLYNIKQLAKDIEKATG", "aa_hexdigest": "ce9d337683932a4658b53dc08bd059e9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A975M207", "query_cov": 0.9795918367346939, "subject_cov": 0.8791208791208791, "identity": 0.5, "score": 233.0, "evalue": 1.75e-73, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A7V6J2C4" }, "pscc": { "uniref50_id": "UniRef50_A0A7V6J2C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "product": "ATP-dependent 6-phosphofructokinase" }, "genes": [], "id": "EFPKPENHBP_1018", "locus": "GCA000186005_00990" }, { "type": "cds", "sequence": "contig_1", "start": 549236, "stop": 549367, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCTCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAAATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAA", "aa": "MAIGIIDNDIVASPISEALSMINKSKAKVRNKAIKFNELNRLD", "aa_hexdigest": "de38ff7d544e9981c8d979ad599baa0f", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.520399999999, "isoelectric_point": 9.397443580627442 }, "id": "EFPKPENHBP_1019", "locus": "GCA000186005_00991" }, { "type": "cds", "sequence": "contig_1", "start": 549594, "stop": 549920, "strand": "+", "frame": 3, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "nt": "ATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTATATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGA", "aa": "MSQKEDKKPIVFSDVDGTLYARDCYVSNFNLNIIKQDNLSFNIATGNPICPRMLKLAKLVYADYMIGSSGVQIYDYKHNKYLHEEYLKASVVKKIFKLFKESKVSAAA", "aa_hexdigest": "d64c9811c405c9286c0bc7cac099e6d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00210774DB", "query_cov": 0.9074074074074074, "subject_cov": 0.875, "identity": 0.7140000000000001, "score": 143.0, "evalue": 2.9e-42, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI00210774DB" }, "pscc": { "uniref50_id": "UniRef50_UPI00210774DB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "product": "HAD family hydrolase" }, "genes": [], "id": "EFPKPENHBP_1020", "locus": "GCA000186005_00992" }, { "type": "cds", "sequence": "contig_1", "start": 550233, "stop": 550418, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAG", "aa": "MVIGDSENDYSMFHKFNYSYVMDNAKDEVKDKANFITKAVQEHGLGYAVMDYLEKFNKRNN", "aa_hexdigest": "d61069b349d6d13fc53c2b8168a08c7c", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 48, "aa_cov": 0.7868852459016393, "hmm_cov": 1.0, "evalue": 1.329379599395558e-15, "score": 54.09297561645508, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 7174.945400000002, "isoelectric_point": 5.50738010406494 }, "id": "EFPKPENHBP_1021", "locus": "GCA000186005_00993" }, { "type": "cds", "sequence": "contig_1", "start": 550734, "stop": 551573, "strand": "+", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEW7", "UniRef:UniRef90_C4XEW7" ], "nt": "TTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAA", "aa": "MLKLGVLSQNIIQINLELEENKKLRKSLNLLNFIKEQIINENKYYVLIDEIQNVKNFEDSVNYLLNIKNVDLYITGSNSKFLSRDIETQISSRATEIKVRPLTFKEFYSVKKELNIDEVFKEYLTFGGMPFLVNLNYESKKKYLKELFETTYINDIVERNKIKSDEYLQEFVDFLTSNVGSLTNNLNICNTYKTLKQNNIAYFTIDKYLKNLSHTYLIDLVQLFNLKGKRIIKRLNKIYFYDLGIRNSRIGYNNIKIEHAIENIVYNELVIRNYDVNIG", "aa_hexdigest": "089c8ef35cfe98923aeaf5bea060da5b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEW7", "query_cov": 1.0, "subject_cov": 0.8353293413173652, "identity": 0.996, "score": 538.0, "evalue": 3.05e-192, "valid": true, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XEW7", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_C4XEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW7" ] }, "genes": [], "id": "EFPKPENHBP_1022", "locus": "GCA000186005_00994" }, { "type": "cds", "sequence": "contig_1", "start": 551888, "stop": 552853, "strand": "+", "frame": 2, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "nt": "ATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGA", "aa": "MARFIDEIKITLQAGKGGDGMISFRREAHVDKGGPDGGDGGRGGNIYFIGDLGKNTLLNLYGNKKITAEDGINGGPKNLYGAAGKDTYVKVPLGTVVYKNNKVVADIIEPNKNYLVAQGGQGGRGNMKFKSPRNTAPRICENGTKGEKFEAHIVLKVMSDVGVVGKPSAGKSTLLSVISNAKAKIAEYEFTTLVPQLGLVRYFDNSFTVADLPGLIKGASLGKGLGFQFLKHIERCRVIAHIIDFGLSEKNPIEDYETINNELASYSMHLENKPQIVVANKSDMPDFKDHLKAFKKKYPKVKVIVISALEQENLDQLKAEL", "aa_hexdigest": "e41655d052ef5ddce95ed7a8d8e7ce4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_Q3XWL6", "query_cov": 1.02803738317757, "subject_cov": 0.9041095890410958, "identity": 0.515, "score": 313.0, "evalue": 3.57e-102, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q3XWL6", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q3XWL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "product": "GTPase Obg" }, "genes": [], "id": "EFPKPENHBP_1023", "locus": "GCA000186005_00995" }, { "type": "cds", "sequence": "contig_1", "start": 552908, "stop": 553072, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09269.16" ], "nt": "ATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGA", "aa": "MVEIKLEDDFTIANPYRGFFEVSGPKVEKIYEKIPLISYDNLIRFNNMLKKIGV", "aa_hexdigest": "ab4751304e8aae3c9754b8556ed596ba", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09269.16", "name": "DUF1967", "length": 46, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.7176576360887484e-10, "score": 37.1574821472168, "start": 10, "stop": 54 } ], "seq_stats": { "molecular_weight": 6337.3634, "isoelectric_point": 6.147955513000488 }, "id": "EFPKPENHBP_1024", "locus": "GCA000186005_00996" }, { "type": "cds", "sequence": "contig_1", "start": 553252, "stop": 553719, "strand": "+", "frame": 1, "gene": null, "product": "Glucosamine-6-phosphate deaminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0", "UniRef:UniRef90_C4XEX0" ], "nt": "ATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGA", "aa": "MAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNF", "aa_hexdigest": "fde3a6b565bab662ecd6ded12682939a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 553234, "stop": 553989, "observations": { "mutation": [ 553717 ], "stop": [ 553717 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX0", "query_cov": 1.0, "subject_cov": 0.6175298804780877, "identity": 1.0, "score": 314.0, "evalue": 4.61e-107, "gene_start": 1, "gene_end": 155, "reference_start": 7, "reference_end": 161, "reference_sequence": "MILRSPMAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNFWLNNDLLNNFKIMQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN" }, "paralog": false, "identity": 0.9920318725099602, "score": 493.0, "evalue": 3.1e-180, "description": "Internal stop codon at 553717. Nonsense mutation around 553717.", "psc": { "uniref90_id": "UniRef90_C4XEX0", "product": "Glucosamine-6-phosphate deaminase", "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX0", "UniRef:UniRef50_C4XEX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0" ], "product": "Glucosamine-6-phosphate deaminase" } }, "id": "EFPKPENHBP_1025", "locus": "GCA000186005_00997" }, { "type": "cds", "sequence": "contig_1", "start": 553753, "stop": 553989, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAA", "aa": "MQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN", "aa_hexdigest": "8465db29de169fbeba7073fa9a33727a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8450.764899999995, "isoelectric_point": 9.901844215393066 }, "id": "EFPKPENHBP_1026", "locus": "GCA000186005_00998" }, { "type": "cds", "sequence": "contig_1", "start": 554023, "stop": 554373, "strand": "-", "frame": 1, "gene": null, "product": "Hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "nt": "ATGACTACTTCTAGTTATGCAGGAGCAGGCAATAGTAGCGGTAGTGTTAAAATCATTATGGACTTGGCTCAAGATATTAGAAATAAAGATGTTTTAATTGTTGAAGACATTATTGATTCAGGAATTACACTTGATAAAATCAAGCAAATTCTTTTATCTCGTAAACCTCGTTCATTTAAAATAATAACTCTTTTAGACAAACCACACAACCGTAAAGTTGAATTGAAAGCTGATAAATCAGCCTTTATCGTGCCAAATGAATTCTTAGTTGGTTTTGGTTTGGATTATGATGAAAAAATGAGAAACCTCCCTTATATTGGAATTTTTGATAAAAAATATTTAAAAAAATAA", "aa": "MTTSSYAGAGNSSGSVKIIMDLAQDIRNKDVLIVEDIIDSGITLDKIKQILLSRKPRSFKIITLLDKPHNRKVELKADKSAFIVPNEFLVGFGLDYDEKMRNLPYIGIFDKKYLKK", "aa_hexdigest": "81af85f6aba0c8dde6570696b4e959be", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PE13", "query_cov": 0.9827586206896551, "subject_cov": 0.8085106382978723, "identity": 0.632, "score": 151.0, "evalue": 6.2e-45, "valid": false, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_A0A3B0PE13", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PE13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "product": "Hypoxanthine phosphoribosyltransferase" }, "genes": [], "id": "EFPKPENHBP_1027", "locus": "GCA000186005_00999" }, { "type": "cds", "sequence": "contig_1", "start": 554491, "stop": 554580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAGCAAAATATTGACTATCGAATTAAAAAGGTTTTATATACTCAAGAAGAAATTGAAGCTAAAATCAAAGAATTAGCAACTTGA", "aa": "MNKQNIDYRIKKVLYTQEEIEAKIKELAT", "aa_hexdigest": "53592a0486315c4d4995180bac52042d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3511.052100000001, "isoelectric_point": 8.064688682556149 }, "id": "EFPKPENHBP_1028", "locus": "GCA000186005_01000" }, { "type": "cds", "sequence": "contig_1", "start": 554573, "stop": 555106, "strand": "-", "frame": 3, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX2" ], "nt": "ATGTATGAAAAACAACCTAATATTCACATTGGTTTTCAATCATATATTATTGAAAGCTTATCAGACACATTTAAAAAAACAGTAGCTAAATTTAAAAATAATAACTTAAATTTTAGAGACAATCATATCTTTTTAGATCGTTTCAACATCGAACACTATGTTTTTGCTGTTGTCACTTTAATGGATAAAGATCCTAAATATCAAGCAGGTTTTGATGCTATGTTTGAAAAAATAGTTGATCCTGAAGATAATCCGGATTTGGCGATCTTTATTGATATTGATTTTAATGCATTCAAAGCTCGTTTATTAGCTCGAGGTCGCGCTTCAGAAGTTGATAATTATGCTCAAAATGAAACATACTTTAAATTACTTCATTCTTTATATAAAAAGCTTTATATAAGATTAGTTGAAAAATTTAAGATACCTTATGTTATCATTAATAGTAATAACAAAAGTGAAAAAGAAGTTTTACAAGAAGCTATTAATATAATAGATAGCTATGACTTCTCAAAATCAAAGAGGTATAATGAATAA", "aa": "MYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE", "aa_hexdigest": "f606b2530d97c1f8c64984f741358fc3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 554573, "stop": 555256, "observations": { "mutation": [ 555109 ], "stop": [ 555109 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX2", "query_cov": 1.0, "subject_cov": 0.7797356828193832, "identity": 1.0, "score": 350.0, "evalue": 2.36e-121, "gene_start": 1, "gene_end": 177, "reference_start": 51, "reference_end": 227, "reference_sequence": "MGLTMIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLKWMYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE" }, "paralog": false, "identity": 0.9911894273127754, "score": 451.0, "evalue": 2.36e-163, "description": "Internal stop codon at 555109. Nonsense mutation around 555109.", "psc": { "uniref90_id": "UniRef90_C4XEX2", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX2", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "EFPKPENHBP_1029", "locus": "GCA000186005_01001" }, { "type": "cds", "sequence": "contig_1", "start": 555107, "stop": 555244, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTGGTATAAGTGGCATGATTGGCAGTGGTAAAAGTACACTTGCTAAAGGCTTACATAAACATTATAAAAAATCAGTTTTATTAGAAGAATTTCAAGAAAATGACCCAGTTTTTAATACATTTTTAAAATGA", "aa": "MIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLK", "aa_hexdigest": "4738f28a360d814ddd5b12c268723004", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5007.825100000001, "isoelectric_point": 9.225441932678223 }, "id": "EFPKPENHBP_1030", "locus": "GCA000186005_01002" }, { "type": "cds", "sequence": "contig_1", "start": 555445, "stop": 555738, "strand": "-", "frame": 1, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX3" ], "nt": "GTGGAAAACCACACATCTAAATTAGCAGATTGTTTTAAAAAATTTAGCGAAAAAGGCTATAAATACAAGGATAATCACATATTTTTAGATCGTTTTAGTATTGAACATTACATTTTTGCTAATGTTAACTTAAGACCTAAAGGTAAAAAATATTTAGAAGGTTATGATGCACTTTTCTCTCATCTTATTACTAAAGATGAAACTCCAGATTTAGCAATTTTTCTAGATATGACTTTTGACACTTTCAAGAAAAGATTATTTGAAAGAGGTAGAGAAGTTGAAACTGAAAACTGA", "aa": "MENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRPKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETEN", "aa_hexdigest": "7baebc4d7e0039080bf186700ffa63c2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 555253, "stop": 555945, "observations": { "mutation": [ 555447, 555789 ], "stop": [ 555447, 555789 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX3", "query_cov": 1.0, "subject_cov": 0.4217391304347826, "identity": 0.9790000000000001, "score": 200.0, "evalue": 1.59e-63, "gene_start": 1, "gene_end": 97, "reference_start": 70, "reference_end": 166, "reference_sequence": "MKKEVIIMVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLKWLYEKQPNLTIGFQSYVVENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRTKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETENWNKNKDYFRELYDLYKPLFIKQAKKYDLNYVIIETDNLTEKQVFEKAVEVINNYDFSKMERYNK" }, "paralog": false, "identity": 0.9826086956521739, "score": 456.0, "evalue": 1.27e-166, "description": "Internal stop codon at 555447, 555789. Nonsense mutation around 555447, 555789.", "psc": { "uniref90_id": "UniRef90_C4XEX3", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX3", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "EFPKPENHBP_1031", "locus": "GCA000186005_01003" }, { "type": "cds", "sequence": "contig_1", "start": 555787, "stop": 555924, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAGGTATAAGCGGAATGATTAGTAGTGGTAAAAGCACACTAACAAGAAATTTAGTTAAACACTACAAAAATTCAATGATGTTAAAAGAATATGAAGAGGACGATGATGTCTTCAATACATTCTTAAAATGA", "aa": "MVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLK", "aa_hexdigest": "ff5e553325210b21fc920c2e7d9c832b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5171.940100000001, "isoelectric_point": 6.530084037780762 }, "id": "EFPKPENHBP_1032", "locus": "GCA000186005_01004" }, { "type": "cds", "sequence": "contig_1", "start": 556033, "stop": 556524, "strand": "-", "frame": 1, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_013354643.1", "SO:0001217", "UniParc:UPI0001A8C247", "UniRef:UniRef100_C4XEX4", "UniRef:UniRef50_A5IYH4", "UniRef:UniRef90_A0A3P8MDY7" ], "nt": "ATGGCAGGTAAAAAGCAAGAAAAAATCATATTAGCTAAAGAAACATTAGAAAAATATCAAGCAGAATATGAACACTTAATTAAAGTTGAAAGACCTGAAATTCAAGCAGCTTTAAAAGAAGCTAGAGCACAAGGTGACCTTTCAGAAAACGCTGAATATGATGCAGCCAGAGATCGTCAAGGTATTGTTGAAGCAAGAATTGTTGAACTTGAAGGCATTTTAGCAAATGCTGAAGTTATTGAAACAAACCAAAACAAAAAAGGTAAACTAAAAGCAGGTATCGGAGCAACAGTTAAATATCTTAACTTAAAAACAAATAAAGAAATTACAGTTAAAATCATGGGTATTCATGATTCTAATCCAATGGAAGGTAATATTTCTAATGAATCACCAGTAGCACAAGCTATCATGGAAGGTGATGAAGGCGCAACAGTTGAAGTTGATGTGCCTAATAAATACAGTATAAAAATCTTATCAATTCAATACAAATAG", "aa": "MAGKKQEKIILAKETLEKYQAEYEHLIKVERPEIQAALKEARAQGDLSENAEYDAARDRQGIVEARIVELEGILANAEVIETNQNKKGKLKAGIGATVKYLNLKTNKEITVKIMGIHDSNPMEGNISNESPVAQAIMEGDEGATVEVDVPNKYSIKILSIQYK", "aa_hexdigest": "9912c554e9a6fd621826c079a4171577", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C247", "ncbi_nrp_id": "WP_013354643.1", "uniref100_id": "UniRef100_C4XEX4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C247", "RefSeq:WP_013354643.1", "UniRef:UniRef100_C4XEX4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEX4", "uniref90_id": "UniRef90_A0A3P8MDY7" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY7", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_A5IYH4", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYH4" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "EFPKPENHBP_1033", "locus": "GCA000186005_01005" }, { "type": "cds", "sequence": "contig_1", "start": 557042, "stop": 557275, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTTCAATTAATTCAACTGAAACAAATCAAGAATTATTTGACAAATATTATCCAATGTTAAGTGAAAAAGGGATGCTTGTTGTATTACAAAATTCAAATACAAAAAGTGGTTATAGAGTTTTAACTAAACATTTAAAACTTAATAAAATTGTTTATGAATTAAGTTATGTAAAAAGCGGATTCTTATATATAGTAAAAAGTAAAACTGAAATAAAAAATGAAAACTAA", "aa": "MIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN", "aa_hexdigest": "dab3e62cf1061dcdea90814a94a25db2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9001.359299999998, "isoelectric_point": 9.363597679138184 }, "id": "EFPKPENHBP_1034", "locus": "GCA000186005_01006" }, { "type": "cds", "sequence": "contig_1", "start": 557369, "stop": 557770, "strand": "-", "frame": 3, "gene": null, "product": "Methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5", "UniRef:UniRef90_C4XEX5" ], "nt": "ATGATTAGTGCTGTAATCAATTTAAGTAAAGGTGCAAAAATTGGCTTAATTGTTTCTGCTATTGTTTTTGTGATTATTGCCTTTATAACTTATATTATTTTACTAGTTAAAACTAGAAAAATTCGTAATGAAATAAATACCAAAGAAGCTCAAAAAGCTCATGAAGCTATTTTGAAAATCAGAGGTGAAGAATTAGGAGAATTCCCTAAAGAATTAAAAGAATTTTTTAAAAGTAATCTTGATGATTATGATTTAGAAAACTTTATTAATACTATTTATTTAAATGATGTTCAAGAGATTTTACTTGTTGGAAGTAAGTTAGAATATCCAACAGCTTTATTTAAAAATAAATCACAAGCTCAAATTTGTTTAGAAAAACAAAAAATGAATGTAAGTTTATGA", "aa": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQKMNVSL", "aa_hexdigest": "e4b96dbd05e991abab0e0df6b49e0905", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 557066, "stop": 557770, "observations": { "mutation": [ 557371 ], "stop": [ 557371 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX5", "query_cov": 1.0, "subject_cov": 0.5495867768595041, "identity": 1.0, "score": 243.0, "evalue": 9.75e-80, "gene_start": 1, "gene_end": 133, "reference_start": 1, "reference_end": 133, "reference_sequence": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQKMNVSLWNKAVLELPKYFKDQPSFINVNELKTQDKKFKLIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN" }, "paralog": false, "identity": 0.9914529914529915, "score": 447.0, "evalue": 2.53e-162, "description": "Internal stop codon at 557371. Nonsense mutation around 557371.", "psc": { "uniref90_id": "UniRef90_C4XEX5", "product": "Methyltransferase", "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX5", "UniRef:UniRef50_C4XEX5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5" ], "product": "Methyltransferase" } }, "id": "EFPKPENHBP_1035", "locus": "GCA000186005_01007" }, { "type": "cds", "sequence": "contig_1", "start": 557763, "stop": 558197, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "EC:3.1.21.10", "GO:0006364", "RefSeq:WP_013354645.1", "SO:0001217", "UniParc:UPI0001E330FB", "UniRef:UniRef100_A0AB32XC51", "UniRef:UniRef50_A5IYK4", "UniRef:UniRef90_A0AB32XC51" ], "nt": "ATGCGAGCTTTAGCTCTAGATTTAGGCACTAAAACTTGCGGTTTTGCAATTAGTGATGAAGGCATGATTATAGCTAGTGCACTTGAAACATTACATTTTGAAGAAAATGATTTTAATGCAATTATTGAGCAAATCAAAATTTACCAAAAAACATATAAAATAGATAAATTGGTATTAGGAAAGCCTTTAAGATCTAATAATACAAGTAGTGAAAGAACAGTGCTTTTTGAATCTTTTGCTCATAAACTAAAAGGTATTTTTAAAGACTACAAAATTTATCAAGTTAATGAATATGGAACTACTATAGCTGCTACTAATATTTTAAAAGAAGCAAAACTTTCTATTAAAAAAAGAAAAGCTCATAAAGATACTTTGTCAGCTGTTTTAATCTTGCAAGAATTTTTAAATTATGGAGGTGTAGAATTACATGATTAG", "aa": "MRALALDLGTKTCGFAISDEGMIIASALETLHFEENDFNAIIEQIKIYQKTYKIDKLVLGKPLRSNNTSSERTVLFESFAHKLKGIFKDYKIYQVNEYGTTIAATNILKEAKLSIKKRKAHKDTLSAVLILQEFLNYGGVELHD", "aa_hexdigest": "e8fd5b429388892961df9e96ae9ae147", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E330FB", "ncbi_nrp_id": "WP_013354645.1", "uniref100_id": "UniRef100_A0AB32XC51", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330FB", "RefSeq:WP_013354645.1", "UniRef:UniRef100_A0AB32XC51" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC51", "uniref90_id": "UniRef90_A0AB32XC51" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC51", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A5IYK4", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYK4" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "EFPKPENHBP_1036", "locus": "GCA000186005_01008" }, { "type": "cds", "sequence": "contig_1", "start": 558197, "stop": 558922, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02272.25", "PFAM:PF07973.19" ], "nt": "ATGGGCGCAATTATGACTATTGACGAAAGTGAATACATGGATCCTAATAACATTCGTATTGTTGACTTTAAAGGTATTACAAAAGACCTTTGCGGTGGTACTCACTTAGATAGAACCGGAATTTTAGAAGACTTTAAAATTACTAAAGTTGAGAAAAAAACTGCTGGTGTATTTAGAATAAGAGCTGTAGCTTCTCATAAATATGTTAAACAATATTTAACAGAATTAAATCAAAGTTTAAAAGAACAATTAGATGCTTTAGTTAAAAAAGCTCAAAGCTTTGATAGTAAATATCAAATTAAATTTACACCTAATAAAGATGTTGAAATTGAATGTGCAAACATTAAAAAAACAATTGATAAAGTTCATGAAGATATCAAAGAATTAATCAAAAACAAAAGCAATATAACCTTTGATTTTGATAGTGTTCAATTAACTAAAAACAAAAAATACCCTTATTATTTAAACATTGATTTTGATAGTTCCCAAGTTAAAGTTGCTGCTGCAACGCTTAGAGAAAAATTTCCTGAAGCTATAATTGTTTTAGGTGCTAAAAGTAATAATCAAATTCTTTTATGTGTGGCCGCTAAAGTGCTTGATGCTAACAAATTATTCCAACAAATAGCTTCAAAAACCAATGGTCGTGGTGGTGGAAGTTCAATTATTTCAATGGGTAAAGTTGACTATACTAAAGATCTTGAAAATATTATTAAGGAACTTATTTAA", "aa": "MGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI", "aa_hexdigest": "7d29e705574c7529a619c84fc12dbbe1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02272.25", "name": "DHHA1", "length": 89, "aa_cov": 0.35269709543568467, "hmm_cov": 0.9775280898876404, "evalue": 2.709858354889229e-09, "score": 33.768638610839844, "start": 156, "stop": 240 }, { "id": "PF07973.19", "name": "tRNA_SAD", "length": 43, "aa_cov": 0.17842323651452283, "hmm_cov": 1.0, "evalue": 1.2370914751411077e-13, "score": 47.12787628173828, "start": 18, "stop": 60 } ], "seq_stats": { "molecular_weight": 27148.053900000024, "isoelectric_point": 8.986135292053223 }, "id": "EFPKPENHBP_1037", "locus": "GCA000186005_01009" }, { "type": "cds", "sequence": "contig_1", "start": 558980, "stop": 560113, "strand": "-", "frame": 3, "gene": "alaS", "product": "alanine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.7", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419", "SO:0001217", "UniRef:UniRef50_Q98Q12", "UniRef:UniRef90_C4XEX7" ], "nt": "ATGCAAGATGCTCCAACTAACTATGACAGCGATCTTTTTATTAACATAATTAAAGAAATAGAAAAATATGCCACAGTTAAATATGACATTGATAACTACTTTAAAAAAGATAAAAAGCAAACTGAAATCAACACTAACTTTAAAGTTATAGCAGACCACATTAGAACAGTGGTTAATGCTATAGCTGATGGAGCTAAAGTTTCAAATGTTGGCCGTGGTTACATTATTAGAAGACTTATTCGACGTTCAATTTACAAAGGAATGCAACTAGGAGTTAAAGGTCTATTTTTACATAAATTAGTAGATGTAGTACAAGATTCATTACCTTATGAATATGACAAAAAACCTGTTGTAGTAGCCATTAAAGAAGAAGAATTAGCTTTCCATCAAACTATTGAAAAAGGTAAATTATTACTTGAAAAATTTATGGATAATAAAACTAAAATTTTCTCAGGTGAAGATGCTTTTAATTTACTTGAAACTTATGGCTTTCCAATTGAGTTAACTGTTGAAATTTTAGCTAAGAAAAATATTAAAGTTGATCTTAAAGCTTTTGAAGAAGCTAAGGAAAGACACGCCAACTTAAGCAGGGGTGAAAAAGTTAACGGAATGGATAAAGTAATTAACTCATTAGCCTTAATTAAAGGCAAAATCGGTGAATTTATTGGTTATACAGACTTAGAAAGTAAATCAAAAATTCTTAAACTTTTAAATAATGAGGAAGAGGTAAAATCATTAAATGGTGAAGGTTATTTGATCTTAGATAAAACACCTTTTTATGCTACTAGTGGTGGTCAAAATCATGACCATGGTTTCATTTTACAAGGTAAAAATAAAATCAAAATTTTAGATGTTTTTAAAGACAAATTTGGTAACCATATTCATAAAATTGAAGGCAAAATTAACAATAAGGATACTGTCGAATGTTATGTTGATAAAGAAGTAAGAATTGGTATGGCTCGAGGTCACTCAGCAACTCACTTATTATTCCGTGCTTTAGTTAAAGTACTTGGACCTCATATTGATCAACTTGGTTCAGATATCACCGAAGAACGTTTAATATTTGACTTTCCAGCTGATGAAAAACCAACTGATGAACAAATTAAAAAAATCGAAGACATAATGCATGGTTGA", "aa": "MQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHG", "aa_hexdigest": "ac67aa2f7429c6039832759aba239c3a", "start_type": "ATG", "rbs_motif": null, "genes": [ "alaS" ], "truncated": "both", "pseudogene": { "start": 558677, "stop": 560413, "observations": { "mutation": [ 558982, 560221, 560347, 560407 ], "stop": [ 558982, 560221, 560347, 560407 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX7", "query_cov": 1.0, "subject_cov": 0.4308571428571429, "identity": 1.0, "score": 744.0, "evalue": 1.2e-263, "gene_start": 1, "gene_end": 377, "reference_start": 238, "reference_end": 614, "reference_sequence": "MTMEKLTSKEIREKWLRYFESKGHLRVESKSLIPVNDPSLLWINSGVATLKDYFSGKKKPPRNRLTNSQKAIRTNDIENVGVTSRHHTFFEMLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKYWMEQGVEESHLIPGTRDTNFWDVGSGPCGPDTEIFYDRGPKYNKRGLELLKKDIENDRYIEIWNIVFSQFNNDGENNYTELKQKNIDTGAGLERIASIMQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHGWIKQNVEREYIVTNIKKAKEMGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI" }, "paralog": false, "identity": 0.9930795847750865, "score": 1137.0, "evalue": 0.0, "description": "Internal stop codon at 558982, 560221, 560347, 560407. Nonsense mutation around 558982, 560221, 560347, 560407.", "psc": { "uniref90_id": "UniRef90_C4XEX7", "gene": "alaS", "product": "alanine--tRNA ligase", "ec_ids": [ "6.1.1.7" ], "uniref50_id": "UniRef50_Q98Q12", "go_ids": [ "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX7", "EC:6.1.1.7", "UniRef:UniRef50_Q98Q12", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q12" ], "product": "Alanine--tRNA ligase" } }, "id": "EFPKPENHBP_1038", "locus": "GCA000186005_01010" }, { "type": "cds", "sequence": "contig_1", "start": 560405, "stop": 560551, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTAACTTTTCAATTGGCGATTACTTTAAAAAAGAAGCTATCGCTTTTGGTTATGAATTCTTAACTAATGAATTACAAATAGACCCTAAAAAACTTTACATGACTTATTTTGCAGAAGATTTGGATACTAAAAAATACTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKY", "aa_hexdigest": "ad41bcfa2a8b765b721ac15ac798e365", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5711.468700000001, "isoelectric_point": 4.7658586502075195 }, "id": "EFPKPENHBP_1039", "locus": "GCA000186005_01011" }, { "type": "cds", "sequence": "contig_1", "start": 560828, "stop": 561547, "strand": "-", "frame": 3, "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "db_xrefs": [ "EC:2.8.1.13", "GO:0008033", "GO:0016740", "GO:0016783", "KEGG:K00566", "SO:0001217", "UniRef:UniRef50_Q600M2", "UniRef:UniRef90_C4XEX8" ], "nt": "ATGGGTCATTATGCCAAAGTTAAGAATGGTAAACTTTATCGGGCGAAAGATCAAAATAAAGATCAAACATATTTTTTAGCTCAATTAACTAATCAACAACTAGAAAAAGTTTTAATGCCTCTTGCTGATTATCCTAAAGAAGAAATAAGAAAAATAGCTAAAAAATTAGACTTAGCTACAGCTACAAAAAAAGATTCAACAGGTATTTGTTTTATTGGCGAAAGACATTTTACAAAATTCTTACAAAACTATATTCCAGCTCAAAAAGGAAAAATATTAGACATTAAAACTAAAGAAGTTTTAGGAACTCATGATGGTTGTTTCTATTACACTATCGGTCAAAGAAAAGGATTAAATTTAGGCGGCAAAAGTGAAGCTCACTATGTTTGCGGTCATGATGTCAAAAACAATATTATTTATGCTGCACCAGCTAGTGATCCTAGTTATTTAGAAAGTAATATGCTAATAGCTTCAAACTTATGTCTAAATCATCATCGCTTTAATAAAAACAAATTAAGTGCTAAATTTAGATATCGTCAAAAAGATCTTAAAGTTAAAGTTGAATTCTTAGAAAATAATAAAATTAAAGTATTTTATCCATCAAAAGCTCAAGCAGTAACCCCCGGTCAACAAGTTGTTTTATATGATCAAGACAAGTGCTTAGGCGGAGCAATAATTGAAGAAATATACTTAAACAATATTAAAAAAGATTACATTTAG", "aa": "MGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI", "aa_hexdigest": "e6786ae432ac4a22986b42ab2e220612", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmA" ], "truncated": "3-prime", "pseudogene": { "start": 560828, "stop": 561847, "observations": { "mutation": [ 561688, 561835 ], "stop": [ 561688, 561835 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX8", "query_cov": 1.0, "subject_cov": 0.6256544502617801, "identity": 1.0, "score": 477.0, "evalue": 3.9e-168, "gene_start": 1, "gene_end": 239, "reference_start": 144, "reference_end": 382, "reference_sequence": "MKLIFNIRINMHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRNWDSYVNNDFLGNENISQDICPQEQDYQDALAVANKLGIKLHRVDFVKEYWDNVFENFIEEYKKGRTPNPDILCNKYIKFDAFLKYAFEKLNADYIAMGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI" }, "paralog": false, "identity": 0.9941002949852508, "score": 687.0, "evalue": 1.85e-253, "description": "Internal stop codon at 561688, 561835. Nonsense mutation around 561688, 561835.", "psc": { "uniref90_id": "UniRef90_C4XEX8", "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "ec_ids": [ "2.8.1.13" ], "uniref50_id": "UniRef50_Q600M2", "kegg_orthology_id": "K00566", "go_ids": [ "GO:0008033", "GO:0016740", "GO:0016783" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX8", "EC:2.8.1.13", "UniRef:UniRef50_Q600M2", "KEGG:K00566", "GO:0008033", "GO:0016740", "GO:0016783" ] }, "pscc": { "uniref50_id": "UniRef50_Q600M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q600M2" ], "product": "tRNA-specific 2-thiouridylase MnmA" } }, "id": "EFPKPENHBP_1040", "locus": "GCA000186005_01012" }, { "type": "cds", "sequence": "contig_1", "start": 561833, "stop": 561946, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCATAAAAAGAAAGTTATTTTAGGAATGTCTGGAGGAGTAGACTCTTCAGTAGCTGCATATTTATTACTTCAACAAGGTTATGATGTTGAAGGTCTTTTTATGCGGAATTGA", "aa": "MHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRN", "aa_hexdigest": "bb6bd5eed5ff4427b49b46d59b44511e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 22, "aa_cov": 0.5945945945945946, "hmm_cov": 1.0, "evalue": 1.9414714963556628e-07, "score": 26.732755661010742, "start": 2, "stop": 23 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.918918918918919, "hmm_cov": 1.0, "evalue": 8.310319361639413e-13, "score": 44.75435256958008, "start": 4, "stop": 37 } ], "seq_stats": { "molecular_weight": 4056.7289999999994, "isoelectric_point": 8.160811042785646 }, "id": "EFPKPENHBP_1041", "locus": "GCA000186005_01013" }, { "type": "cds", "sequence": "contig_1", "start": 562638, "stop": 562727, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTTTAACTATTGCTCGTGCTGTTAATATTGTTTTAGAAAACGGATTAAGACTTTTAGGTGTTAGTTCAAAACACAAAATGTAA", "aa": "MNSLTIARAVNIVLENGLRLLGVSSKHKM", "aa_hexdigest": "10bc86e11d2336a6882e93b0147c64d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3165.7748, "isoelectric_point": 11.000127601623536 }, "id": "EFPKPENHBP_1042", "locus": "GCA000186005_01014" }, { "type": "cds", "sequence": "contig_1", "start": 562767, "stop": 562952, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "TTGCTTAAGAAATCTAAATTAGATGTTTCAAAACTTAAAGCAACAAATATTGACAACGAACTTGATAAAAAATTAGTTTCGACATTGCTTGAATTTCCAGAAGTTATCATTAGTTCAGCAACTAAGTACTTAACACACTTACTAACACAATACTTAATTAGAGTTGCCAAAGAATTTAACTCATGA", "aa": "MLKKSKLDVSKLKATNIDNELDKKLVSTLLEFPEVIISSATKYLTHLLTQYLIRVAKEFNS", "aa_hexdigest": "67f166eb88701eb9e028287d6f393c8a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 62, "aa_cov": 0.9836065573770492, "hmm_cov": 1.0, "evalue": 3.5598562278353634e-10, "score": 36.31890106201172, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6981.156399999999, "isoelectric_point": 9.34754505157471 }, "id": "EFPKPENHBP_1043", "locus": "GCA000186005_01015" }, { "type": "cds", "sequence": "contig_1", "start": 563115, "stop": 563243, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGCAATGCAAGACTTAGGTTACGACCCAAACAAATTAATTGTTCTTTGTTTACAATTAGTAAGACTTATGAAAGATGGTCAAGAATACAAAATGTCAAAACGTAAAGGTACAAGTTTTTGA", "aa": "MKIAMQDLGYDPNKLIVLCLQLVRLMKDGQEYKMSKRKGTSF", "aa_hexdigest": "c6255dd01225134f784e6a40e94c48ce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8623, "isoelectric_point": 9.579889106750485 }, "id": "EFPKPENHBP_1044", "locus": "GCA000186005_01016" }, { "type": "cds", "sequence": "contig_1", "start": 563673, "stop": 564266, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGGATAAAAACATTATTAAAAGCAAAATTATTAAAGAATTAAAACCAATTTTAAACAAATTAGGGATTGAAAAGGACGATCTCTTAATTGAACCTAAAGTCAAAGCCGATTTCGCTTCTAACGTTGCATTAGGAAACAAAGGCAAAAATCCTATGGAACTTGCCAAAAAAATTGTTAAAGAATTAGAGCCTAAACTAACTTCATTAGGTCTAAAAAGTGTAGAAATTGCAGCTCCTGGATTTTTAAACTTTTACATTGATGATCAAACAATTATTAAAAGTTTGAACCAAATTATCAAACAAAATGAAAACTTTGGTCGTGGTAAATTAAAAGGAAATCTAAACATCGAATGGGTTTCAGCAAACCCTACTGGATACTTGCACATTGGTCATGCACGTAATGCTGCTATTGGATCAGCACTATACAACATTTGTACTTTCGCAGGTTTAAATGTTTTACGTGAATATTACATCAATGATGGTGGTAACCAAATTAACACTTTAGCTCAAAGTATATTTGCAAGATATAAACAATTTTATGAACCAGATTTCCCTATGCCAGAAGACTGCTATGGAGGTCAAGACATTGTTTGA", "aa": "MDKNIIKSKIIKELKPILNKLGIEKDDLLIEPKVKADFASNVALGNKGKNPMELAKKIVKELEPKLTSLGLKSVEIAAPGFLNFYIDDQTIIKSLNQIIKQNENFGRGKLKGNLNIEWVSANPTGYLHIGHARNAAIGSALYNICTFAGLNVLREYYINDGGNQINTLAQSIFARYKQFYEPDFPMPEDCYGGQDIV", "aa_hexdigest": "34b945c66ddadab51a07dedeb599802f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 87, "aa_cov": 0.41116751269035534, "hmm_cov": 0.9540229885057471, "evalue": 3.0772538200277005e-13, "score": 46.527488708496094, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 21959.130199999992, "isoelectric_point": 8.692804145812985 }, "id": "EFPKPENHBP_1045", "locus": "GCA000186005_01017" }, { "type": "cds", "sequence": "contig_1", "start": 564538, "stop": 564873, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0", "UniRef:UniRef90_C4XEY0" ], "nt": "ATGTTTAAGAAAAAACTATTAATATCAAGTTTAGCAGTTGCTTCATTTTTACCTGTATCAGTTGTTTGTGTCTCTTGTGGAGATGGTAAAACTGATGAAGAAAAATACTTAGAAGCTGCTAAAGTTAAATTTGAAAACATTAAAGCATATTTAGCCAAAATAAATGACTCTTATGAAGAGTATCAAAAAACTACAAAAATAACTGATTCTGCAATTATTGCTAAAAGAGATAAAGCTAAAAAACTTGGCGAAAAAATGAAATCTGAAAATTACTACAGCGAATTAATTAAAGACAAAAAAATAATAGAAATAAATACTATTTTAAATGACTTATGA", "aa": "MFKKKLLISSLAVASFLPVSVVCVSCGDGKTDEEKYLEAAKVKFENIKAYLAKINDSYEEYQKTTKITDSAIIAKRDKAKKLGEKMKSENYYSELIKDKKIIEINTILNDL", "aa_hexdigest": "72eec781810d4f72e02ba356db2a85c2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEY0", "query_cov": 1.0, "subject_cov": 0.8740157480314961, "identity": 1.0, "score": 208.0, "evalue": 9.2e-68, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEY0" }, "pscc": { "uniref50_id": "UniRef50_C4XEY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0" ], "product": "Lipoprotein" }, "genes": [], "id": "EFPKPENHBP_1046", "locus": "GCA000186005_01018" }, { "type": "cds", "sequence": "contig_1", "start": 564890, "stop": 565222, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTACTTGCAGGCGTTAACAAAGATTATGTTGACTATTCAGTAGACAGTAGTCTAGGAAATGCAACACTTGAAGAATATGTAAAAGCTATTGATGCTTCAAGAGCTGAAATGTACAAAAACTTAATTATTTCTAGAGTTGCATTAGTATGTGTATTAGGAATATTTGCAATGTTAACATTCCTTCCTGCAGTTATTGAAGATGCAATTAACAAGAAAAAATATGGTTCTTTAGAAACTTATGAAGAATACAAAGCACAAAAATTAGCAGAATTGTCAAAAGAAGAAGCTACTTTAACTAAACAAAAACCAGTTGAAGTTCAAGCTTAA", "aa": "MFLLAGVNKDYVDYSVDSSLGNATLEEYVKAIDASRAEMYKNLIISRVALVCVLGIFAMLTFLPAVIEDAINKKKYGSLETYEEYKAQKLAELSKEEATLTKQKPVEVQA", "aa_hexdigest": "25fdb73ff790f550619a8d5400bec70e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12260.048999999988, "isoelectric_point": 4.990429496765136 }, "id": "EFPKPENHBP_1047", "locus": "GCA000186005_01019" }, { "type": "cds", "sequence": "contig_1", "start": 565658, "stop": 566050, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCCAGATCAAACATTTGCAGAAGCAATTAAATCAGGTAAAAGTGTTACTGCTTTACCTAAATTAGGAGCTGGTCTTGGTGTATTCCTTGCTGTTGCTGCTATCTTCTTTGCTTATGATGGATTCTATGTCGCTGCTGGAATTCAAAGTGAAATGAAAGAACCTAAAAAGACTCCTTTAGCAATTTTCATTGGTTTAGGAATCACAACCTTAATTTACTTAATTGTTGCTATTTCAATGTCACTTAACGGTGGATCATTCTTTGGCTTCAATGGATACATGAGTAGTTTATTCAAAAATGAAAAAGCTGGTCGAATTGTTTTTGGTTTAATTAACATTTGTATTTCAATTGGTGTTCTAGGTATTATTAATGGTTTCTCAATGTGA", "aa": "MPAPDQTFAEAIKSGKSVTALPKLGAGLGVFLAVAAIFFAYDGFYVAAGIQSEMKEPKKTPLAIFIGLGITTLIYLIVAISMSLNGGSFFGFNGYMSSLFKNEKAGRIVFGLINICISIGVLGIINGFSM", "aa_hexdigest": "0a0cda4308aec5277add6979894ad13c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.043899999986, "isoelectric_point": 9.13157596588135 }, "id": "EFPKPENHBP_1048", "locus": "GCA000186005_01020" }, { "type": "cds", "sequence": "contig_1", "start": 566243, "stop": 566368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTATATCTATTTACCTCTTACATACTTATTTATGCCTATGTATGTAATCATGTCACTTCAAGATGGTGTTGGGGCATTAATGAATAAAGATGCTTTAACATTCGGAACAAATGCTGACTGA", "aa": "MTYIYLPLTYLFMPMYVIMSLQDGVGALMNKDALTFGTNAD", "aa_hexdigest": "8845ad847763da78011822c1c28973e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4624.438300000001, "isoelectric_point": 4.16927661895752 }, "id": "EFPKPENHBP_1049", "locus": "GCA000186005_01021" }, { "type": "cds", "sequence": "contig_1", "start": 566507, "stop": 566659, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAAAACAAACAAAAGAAAAGAAGATTTCCTTTATAGCTGCTATGTTGATCGTTATAGGTGGATCAATTGGTGCTGGTATCTTCTTTAAATCAGGTGCTGTTTTAAAAAGCTCGCACAGTAGTTTGATTCTAGCCATTTTCTGTTGA", "aa": "MEEKQTKEKKISFIAAMLIVIGGSIGAGIFFKSGAVLKSSHSSLILAIFC", "aa_hexdigest": "9af944d4ff5ad9f2f66088e98547419b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.333899999998, "isoelectric_point": 9.430902671813964 }, "id": "EFPKPENHBP_1050", "locus": "GCA000186005_01022" }, { "type": "cds", "sequence": "contig_1", "start": 566713, "stop": 567645, "strand": "-", "frame": 1, "gene": null, "product": "Carbamate kinase", "db_xrefs": [ "RefSeq:WP_013354651.1", "SO:0001217", "UniParc:UPI0001E33100", "UniRef:UniRef100_C4XEY2", "UniRef:UniRef50_P78030", "UniRef:UniRef90_C4XEY2" ], "nt": "ATGTCAAAAATTGTTATAGCTTTAGGTGGAAACGCTTTAGGAAACAATCCTGAAGAACAAAAAGAATTAGTTAAATTACCTGCTAAAAAAATAGCAGATTTAGTTAAAGAAGGTCACCAAGTGCTTGTTGGTCATGGTAATGGTCCTCAAGTTGGAATGATCTTCAATGCCTTTGCTGATGCAAAAAAAGTTAATGAAAAAACTCCATTAGTACCTTTTGCAGAAGCAGGTGGTATGTCTCAAGGCTACATTGGTTATCACATGCTCACAGCGATAACCAATGAATTGAGAAAGAAAAAAATTGAAAAAGATGTTTTATACTTTTTGACTCAAACAATAGTTGATGCTAAAGATAAAGCATTTAAAAACCCTACAAAGCCAGTTGGTCCTTTCTATAAAACTTTAGAAGAAGCTGAATTGGCTAATCCTAATTCAACCATTAAAGAAGATGCTGGTCGTGGTTTTAGAAAAGTTGTTCCTTCACCACTTCCTCTTGACTTTATTGGAATGAAAACAATTAGAAAAACATTTGACGCTGATAATGTTGTTATAGTTGGTGGTGGTGGTGGCATACCTACTATCGACGAAAAAGGACAATATAAAGGTGTTGATGGCGTTATCGACAAAGATTTCGCTTTAAGTAAATTGGCTACAAAAGTTGAAGCAAACTTATTTGTGATTCTTACAGCAGTAGAACATGTTTGTGTTAACTTTGGTAAACCAAACCAAAAAGCTTTAAAAGAAGTAACTGTTTCTGAACTTGAACAATACATCAAAGAAGAACAATTTGCTCCAGGAAGTATGCTTCCTAAAGTTAAAGCTGCTATTGCTTTTGTTAACGAAAATCCAAAAAATGTAGCTGTTATTGCTGACTTAAACAAAGTATCACAAGCTATCGAAGGCAAAAGTGGTACAAGAATTATCAAAGGATAA", "aa": "MSKIVIALGGNALGNNPEEQKELVKLPAKKIADLVKEGHQVLVGHGNGPQVGMIFNAFADAKKVNEKTPLVPFAEAGGMSQGYIGYHMLTAITNELRKKKIEKDVLYFLTQTIVDAKDKAFKNPTKPVGPFYKTLEEAELANPNSTIKEDAGRGFRKVVPSPLPLDFIGMKTIRKTFDADNVVIVGGGGGIPTIDEKGQYKGVDGVIDKDFALSKLATKVEANLFVILTAVEHVCVNFGKPNQKALKEVTVSELEQYIKEEQFAPGSMLPKVKAAIAFVNENPKNVAVIADLNKVSQAIEGKSGTRIIKG", "aa_hexdigest": "9389ce12ac167fa28f76f2a1b13e5d56", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33100", "ncbi_nrp_id": "WP_013354651.1", "uniref100_id": "UniRef100_C4XEY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33100", "RefSeq:WP_013354651.1", "UniRef:UniRef100_C4XEY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEY2", "uniref90_id": "UniRef90_C4XEY2" }, "psc": { "uniref90_id": "UniRef90_C4XEY2", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [], "id": "EFPKPENHBP_1051", "locus": "GCA000186005_01023" }, { "type": "cds", "sequence": "contig_1", "start": 567702, "stop": 567950, "strand": "-", "frame": 2, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGAAAATGATTAAAGCTGCAAAAGAAGACGTTATCTTCTTACACTGCTTACCAGCTTTCCACGATGACCACACATTATTCTCAGCTGAAATCAAAGAAAAATTTGGTAAAAAATACCCAGTAGTTGCAACTGGAGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATGCAACAGATCAATTGATCAAGCTGAAAACCGTTTACACACAATTAAAGCTGTTGTTTTAGCAACTATTGGTTACTAA", "aa": "MKMIKAAKEDVIFLHCLPAFHDDHTLFSAEIKEKFGKKYPVVATGAMEVTDEVFQSKCNRSIDQAENRLHTIKAVVLATIGY", "aa_hexdigest": "3b36ec5ac8edc23a46c8307d9ab284c0", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 80.5, "evalue": 3.27e-18, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "EFPKPENHBP_1052", "locus": "GCA000186005_01024" }, { "type": "cds", "sequence": "contig_1", "start": 568020, "stop": 568325, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGTTGGCAGACTATATGACAATTAAGGAATTCAAAGGTGATCTTAAAGGCAAAAAAATTGTCTTTGCTGGAGACATCAAAAATAATGTTGCTCGTTCAATAATGATTGGTGCTGCTTTTACAGGAATGAACTGTGTTCTATGCGGACCTAAAGAACAAGCAAAAATTGTAAAAACTGGAAAAGGTTACAAAGAAGTTTACGAAAAATGTCTAGAATTATTCAAACGTAATGGTGGATCAATTACATTTGAAGACGACAAAATCAAAGCTGCTAAAGATGCAGATGCAATCTATACAGATGTTTGA", "aa": "MLADYMTIKEFKGDLKGKKIVFAGDIKNNVARSIMIGAAFTGMNCVLCGPKEQAKIVKTGKGYKEVYEKCLELFKRNGGSITFEDDKIKAAKDADAIYTDV", "aa_hexdigest": "83345eb2642fcf610372ace96df904cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.8514851485148515, "hmm_cov": 0.9186046511627907, "evalue": 1.6649405915677676e-18, "score": 63.5078125, "start": 16, "stop": 101 } ], "seq_stats": { "molecular_weight": 11120.914499999988, "isoelectric_point": 8.942103385925293 }, "id": "EFPKPENHBP_1053", "locus": "GCA000186005_01025" }, { "type": "cds", "sequence": "contig_1", "start": 568359, "stop": 568745, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAACTTAAAAGGTAGGAGTTTAGACTCAGCTCTAAATTTCACTACAGATCAAATTAATGCACTTATTGACCTTGCTATTGATTTAAAAAGATCAAAAAGACAAGGGTTACATGCTAACAATCGTCCACTTGTTGGAAAAAACATTGCTATCATGTTCCAAAAAGACTCTACTAGAACACGTTGCGCCTTTGAAGTGGCAGCAGCTGACTTAGGAGCATCTTGCACATATATCGGACCTGCAGGTTCTAACTTTGGTAAAAAAGAATCTATCGAAGACACTGCACAAGTTTTAGGAAGATTTTATGATGGTATCGAATTCAGAGGATTCAAACAATCTGATGTTGATGCGTTAGTTAAATATTCTGGAGTTCCAGTTTGA", "aa": "MPLNLKGRSLDSALNFTTDQINALIDLAIDLKRSKRQGLHANNRPLVGKNIAIMFQKDSTRTRCAFEVAAADLGASCTYIGPAGSNFGKKESIEDTAQVLGRFYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "7de699fd2dd1b14b4ad631581d29788d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.586, "score": 144.0, "evalue": 6.2e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 6.11879633638672e-31, "score": 103.83216094970703, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 13940.724999999984, "isoelectric_point": 8.974337577819824 }, "id": "EFPKPENHBP_1054", "locus": "GCA000186005_01026" }, { "type": "cds", "sequence": "contig_1", "start": 568836, "stop": 569099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCTATCTTAATTCCTATCGCAGGTAAAGGAGCAAGTCAATTAGATATTGATATCGAAACTCACTTCGATGGTACAAACTACTTGACAATTGCACCTAGTGTTGTAGTTGGATATTCAAGAAATGAAAAAACTGAAAAAGCTCTTAAAGCAGCTAAAGTTAAAGTTTTATCATTCGAAGGAAACCAACTTTCATTAGGCATGGGTTCAGCAAGATGTATGTCAATGCCTCTTATTAGAGAAGACATTAAGAAAAAATAA", "aa": "MKPILIPIAGKGASQLDIDIETHFDGTNYLTIAPSVVVGYSRNEKTEKALKAAKVKVLSFEGNQLSLGMGSARCMSMPLIREDIKKK", "aa_hexdigest": "2f2c5d3fcf96a4992e30421335741837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9464.039899999998, "isoelectric_point": 9.373719215393066 }, "id": "EFPKPENHBP_1055", "locus": "GCA000186005_01027" }, { "type": "cds", "sequence": "contig_1", "start": 569247, "stop": 569345, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAAAATAGCAGCGGACAAAGAAGTTAAATTCAAAAGAATTTATGCAATCAATGTTCCTCCTATGCCAAACTTAATGCACTTAGATACATGA", "aa": "MARKIAADKEVKFKRIYAINVPPMPNLMHLDT", "aa_hexdigest": "c57cbf45ae18dee8a6fdae57809795db", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3712.4553000000014, "isoelectric_point": 9.819775962829588 }, "id": "EFPKPENHBP_1056", "locus": "GCA000186005_01028" }, { "type": "cds", "sequence": "contig_1", "start": 569457, "stop": 569672, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTATCGACAAAAAAGAACTTGGTATCGAATTAGATAGAGATCTTGTAGTTGACCCTATGCCAAACTTATACTTTACACGTGATCCATTTGCATCAGTTGGTAATGGTATTTCATTACACCACATGAAATATGTCACAAGACAAAGAGAAACAATATTCTCAGAATTTATCTTTGACAATAACCTTGATTACAATACAGTACCTCGTTGA", "aa": "MAGIDKKELGIELDRDLVVDPMPNLYFTRDPFASVGNGISLHHMKYVTRQRETIFSEFIFDNNLDYNTVPR", "aa_hexdigest": "57e3b907be017ce5528ff33462303180", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8262.283799999994, "isoelectric_point": 5.127866172790527 }, "id": "EFPKPENHBP_1057", "locus": "GCA000186005_01029" }, { "type": "cds", "sequence": "contig_1", "start": 569778, "stop": 570050, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTAATGTTTACAGTGAATACGGAAAACTTAAGGAAGTACTTGTTCACACACCTGGAGACGAAATTAGGCGCATCGCGCCTTCTCGCCTCGATGAACTATTATTTTCAGCAATTTTGGAACCAGATTCTGCAATTGCGGAACATAAAAGATTTGTTCAATTATTAAAAGATAACGGAATTAAAGTTATTCAATTGGACGAATTATTCGCTAAAACTTTTGACTTGGTTAGCGAATCAGTTAAGCAATCATTAATTGAAAGATGA", "aa": "MKKINVYSEYGKLKEVLVHTPGDEIRRIAPSRLDELLFSAILEPDSAIAEHKRFVQLLKDNGIKVIQLDELFAKTFDLVSESVKQSLIER", "aa_hexdigest": "5d92807252daa81b9ba8f499ed4ff6ce", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10324.860799999991, "isoelectric_point": 6.101233863830565 }, "id": "EFPKPENHBP_1058", "locus": "GCA000186005_01030" }, { "type": "cds", "sequence": "contig_1", "start": 570346, "stop": 570492, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATGATTATGACATATGATTTTATTATGGAAATAGTAGCTAGTGTATTTCCTAAAATTTACGAAAACGAGTATAAATTAAAGGAAAACCAATATTATGATTTTGATCCTAATAGCTTAGAATTAGTAATCAAAAACAACTAA", "aa": "MAMIMTYDFIMEIVASVFPKIYENEYKLKENQYYDFDPNSLELVIKNN", "aa_hexdigest": "ed2ff75802bea58ddd481f1811b27e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5816.648800000003, "isoelectric_point": 4.333427619934083 }, "id": "EFPKPENHBP_1059", "locus": "GCA000186005_01031" }, { "type": "cds", "sequence": "contig_1", "start": 570700, "stop": 571281, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATCCTTATATTCAAATCACTCACCAAGTTTCAATTATTGAAACAATTAATGGACAAATAACAAATAAAAATAATATTGTTAAAGACCCTAAAAATATCACGATTTATGATCTTCTAGACAATATTATTGCTGTTTATTCAAATAAAGCTGACTACTACATTGTTTATAACAAAGGTTATGAAAATACCAGAAACAATGAACTTATAGAATTAGCTAAACTTTATTATGCTGATAGTCAACATAAAAAAGACATGGAACACTATTTATCTATAAATAAGCTCGATATGGAAAAATTAGAACAATTAATTACTTATGTTAATGAACATACTATTGACTTATTAGATTGTTTTAAACCAATATCTAAGAATAAATTCGATGAACGTTATGGCATGGATGCTAATAAAAATTATTATGTTTTTAAAACTAATTATGTCCCTCAAGCTAAATTTCATAGTGAAAATTTAAATAAAGAAATTTTATATACTTCAGTTTCTAAAAATAGTATTCCTTTTAACGTTGAAAATTTAGCAACTTACTATAAAGACGAAAGATTTTCAACTTTCATTTATGAATTATGA", "aa": "MNPYIQITHQVSIIETINGQITNKNNIVKDPKNITIYDLLDNIIAVYSNKADYYIVYNKGYENTRNNELIELAKLYYADSQHKKDMEHYLSINKLDMEKLEQLITYVNEHTIDLLDCFKPISKNKFDERYGMDANKNYYVFKTNYVPQAKFHSENLNKEILYTSVSKNSIPFNVENLATYYKDERFSTFIYEL", "aa_hexdigest": "5dc021c36ce31ad97f9460d4e05b8c47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 125, "aa_cov": 0.6217616580310881, "hmm_cov": 0.768, "evalue": 1.5001622900455403e-13, "score": 47.455665588378906, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 22937.642800000016, "isoelectric_point": 5.750025749206543 }, "id": "EFPKPENHBP_1060", "locus": "GCA000186005_01032" }, { "type": "cds", "sequence": "contig_1", "start": 571426, "stop": 572046, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTAAAACAACTAATAAAGATTATTTAGAAGCTAATTACTTATATCAAGTAGCTAAAAAAGCCAAAGTTGAAGTTAAAAAAATTCAAAATATTATTTTTGATGACTATACTCAAAATGGTTTTGTTTTTAGAAAAGATAAACTAAACTTAAGATTAATTGAAGCTTCAAGTTGTGGTAAAAACAAAATGGACAAAAAGGCTTTAAGAAGTGATCTTGGTTACACAATTAATGATGGTTCTTATTTTGCTTCAAAAAATGAAGCTAATGTTCATGGAGATTACCTTTTTGCCGCACTTCAAGAAGATATTTTCACTCAACCAAGTGCACGCAAAATGCCAAAAACTTCTTTAGAATTTATGAGCAAAGATCCAAGAAGTAAATTTGGAGACTTTAAAGAAGTAATAAGTAAAATTGTAGAAGCTTATTATTTAACTAAACCAACATTTTCATTTGATAATCAAATTATGTTGCCGGATTCAGATATGTTTTATGGTAAAAACAAAGATTTAATGAATAAAGTTAGAGTAGCTGTTACACCTCTTGAATTACAATATTCTCCTGCTGTCAAAAAATGTTTAAAAACTTTTGATCAAGAATACTTAAATCAATTTTGA", "aa": "MNSKTTNKDYLEANYLYQVAKKAKVEVKKIQNIIFDDYTQNGFVFRKDKLNLRLIEASSCGKNKMDKKALRSDLGYTINDGSYFASKNEANVHGDYLFAALQEDIFTQPSARKMPKTSLEFMSKDPRSKFGDFKEVISKIVEAYYLTKPTFSFDNQIMLPDSDMFYGKNKDLMNKVRVAVTPLELQYSPAVKKCLKTFDQEYLNQF", "aa_hexdigest": "32560bdf123e582d890723fb91325331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23845.065899999998, "isoelectric_point": 9.1501428604126 }, "id": "EFPKPENHBP_1061", "locus": "GCA000186005_01033" }, { "type": "cds", "sequence": "contig_1", "start": 572738, "stop": 572881, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAGCTGCATTCTTAAAAGAAGAAGACTACGCCGATCCTGAAAACTTTCCAATTAATGAAAGATATTTTGCAACTTATCCTTTTAAAGTTGATAAAAAAATGGCTGAAGATGATGCAAATACCATAATTGAATTTTGA", "aa": "MIAAAFLKEEDYADPENFPINERYFATYPFKVDKKMAEDDANTIIEF", "aa_hexdigest": "59a9b8a7fb826e66fc6d94f67f779798", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.117300000002, "isoelectric_point": 4.214918327331543 }, "id": "EFPKPENHBP_1062", "locus": "GCA000186005_01034" }, { "type": "cds", "sequence": "contig_1", "start": 572963, "stop": 573487, "strand": "-", "frame": 3, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E", "UniRef:UniRef90_UPI000FF8613E" ], "nt": "ATGAACAGAGTTAAAAAATTAACAAAAAGAAAATTCTATAATGGACAAGATTACATTTTAGATGAAAGAAAACAAACAATTAAATTAACAACCAATTTTCATAACACACTTTTAAATAGTTTAGGAACTTTTAATGGTTTCAAAAAATTTGGTGGTAGCTCAATTGGTAACGTCTTAGAAACAGATAGCTTCAAAAGTCATTTCCAAGCTTTTTGTTTTATGTCAAGATTAAGTATGCCAGTGTTACAAACTAAATATATAGATGCCGGGGTAGCTATAGAACCTAAAGTTTTTGAACTTATGCAAAATAAATTTAATGAAATGCATCAAAAAGATATTAAAAGAGGTAAAGTTAGTGCTGATAGTCAACCAACAAAAGTAATTCATATTGAAGCAGCAAATGTCGGATATGACTATTTTAAAGGCAAACACGACATTTTAGGTGGTGTTCCTGATGGTCTTATTCCTTCAAAAAATATTGTAGTTGAAGTTAAAACTGTTCAAGAAAAGAAAAAGGTTGATTGA", "aa": "MNRVKKLTKRKFYNGQDYILDERKQTIKLTTNFHNTLLNSLGTFNGFKKFGGSSIGNVLETDSFKSHFQAFCFMSRLSMPVLQTKYIDAGVAIEPKVFELMQNKFNEMHQKDIKRGKVSADSQPTKVIHIEAANVGYDYFKGKHDILGGVPDGLIPSKNIVVEVKTVQEKKKVD", "aa_hexdigest": "5ee56e127bb1e91fcf8a2a23d9b46c64", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF8613E", "query_cov": 1.0, "subject_cov": 0.8877551020408163, "identity": 0.971, "score": 336.0, "evalue": 3.06e-116, "valid": true, "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_UPI000FF8613E" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF8613E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E" ], "product": "MAGa7180 family putative nuclease" }, "genes": [], "id": "EFPKPENHBP_1063", "locus": "GCA000186005_01035" }, { "type": "cds", "sequence": "contig_1", "start": 573677, "stop": 574453, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7", "UniRef:UniRef90_C4XEY7" ], "nt": "ATGCCTTATAAAGCTATTATTCAAAAAATATCTGAATGTGAAGAAAATAAAACAAATGTAACAAGTGTTAAAGAATTAGAAAAAATTAATGTTGAAGATGTCATTAGAATCGACGATGAATTTTTAGATCAAAAAATATTCAACAATAAAAACTTCTTATTTTTCGATTTTGAATCTAGATTAACAAAATTAAATGATAAAGTGGCAATTAGTTACTATCCAGTTCAATATTCTTCTTTTATATCAAACTTAAACAATAAAGAATTTAATGAAAAAGAAAGAAAAAACTTTTACAAAGGTGTAGTTAAAGACAACCGTAAGTTTTTCCAAATTGATGGTTTCATTTTAGATCAATATAGCAATATTTATCAAGATCAAAGTCAAAATTTAATAACAAGAGAAGAAGTTCAAAATATTTTAGATGCTAACAAAATCTTAATTTCTTATGGCATTAAAGCCGATTTAAACAACTTGAAAAAAATTGTAGGTAAAAAATATGTTGAAAGTCGATTAGTCAATTATATTGATGTTTGCAATTTTACAATGATAATTAAAAAATTAATTAGTAAAGAAAAAGAAACAATGTATAGACAAACATTATTTAATGTGGCAAAATATTTTTGTACTTATGATGATGCTTTACTTAGTGAAGAATTAAAAGAAGAATTAAAAAATAAAGATAAAAAAATAAATATACTTTTTCAAGAAGAATATAAATTTGAAAAATTGAATATGCATGATTCATTTGAAGATTCAAGAATCTTAACAATTCTTTGA", "aa": "MPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTIL", "aa_hexdigest": "b03ec4737b699f792aeab9e6971cef24", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 573500, "stop": 574753, "observations": { "mutation": [ 573541, 573679, 574462 ], "stop": [ 573541, 573679, 574462 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEY7", "query_cov": 1.0, "subject_cov": 0.5720620842572062, "identity": 1.0, "score": 494.0, "evalue": 1.35e-173, "gene_start": 1, "gene_end": 258, "reference_start": 135, "reference_end": 392, "reference_sequence": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKNWHKMPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTILWNNVVHRFLQRELCNNLFYKKSIDDNDSKVEKRIETFRDLIFAIKYWLDKIYKEENSTN" }, "paralog": false, "identity": 0.9928057553956835, "score": 821.0, "evalue": 7.27e-305, "description": "Internal stop codon at 573541, 573679, 574462. Nonsense mutation around 573541, 573679, 574462.", "psc": { "uniref90_id": "UniRef90_C4XEY7", "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEY7", "UniRef:UniRef50_C4XEY7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30831.941400000054, "isoelectric_point": 6.098789787292478 }, "id": "EFPKPENHBP_1064", "locus": "GCA000186005_01036" }, { "type": "cds", "sequence": "contig_1", "start": 574460, "stop": 574855, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAATATAAACGGAACAATTAAAAAGACTTTTATCGAAGTTATTGAAGAATTAGAAAATAACCCAAGTATTTTTTCTAAAAAACAATGTTATGAAACACAAGGCTACATATTTAATAAAAGCTTAACGGCTGATAAAGATAATAATCAATTTATTTCTTTAATAGTTGGAGATGAAATTGATACTAATGATTTCTTTGAACTTAAATTCTTTTATTATCCAAAAGATAAAAATAAAACTGATAAAGAAAAATCATATTCTGTAATTAAAAAAATATTTGGTTTCAAAGTACAAACTAAAACTAAACAAGAAAAACAAAATGAAGCAAACAAAAGACAAAACGATTTAGTTAAATATAGTTTTGTTTATACAGTTTCAAAAAATAAAAATTGA", "aa": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKN", "aa_hexdigest": "993e24a5051a319a611a075aabe5fd88", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15458.295499999987, "isoelectric_point": 9.128030204772951 }, "id": "EFPKPENHBP_1065", "locus": "GCA000186005_01037" }, { "type": "cds", "sequence": "contig_1", "start": 574971, "stop": 575726, "strand": "-", "frame": 2, "gene": null, "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961", "UniRef:UniRef90_A0A3P8LBF2" ], "nt": "ATGAAAGGTAAAAGAGTTGTTATTAGAACACTAGATATTGGTGGTGATAAAACTCTTAAATACTTTACATTCCCTCACGAAATGAACCCATTCTTAGGTTATCGTGCAATAAGACTTTGTCTTAAAGAAATTGAAATCTTTAAAACTCAACTTAGAGCTTTAGTTAGAGCAAGTGCTTATGGTAAAGTTGCTATTATGTTCCCAATGATAACAAACATTCCAGAATTCTTGCAAGCTAAAAAACTTTATGAAGAAGCTTATAGTGAAGTTAAAAAAGCAGGACACAAAATTGCTCCTAAAAAGGATATTGAAGTAGGTCTTATGATGGAAACCCCTGCTGCTGCTGTATTAAGCGATCAATTCTGTAAATATGCAGACTTTGTTTCGATTGGAACTAATGACTTAATTCAATACTCAATGGCTGCAGATAGAATGAGTGAAACTATTTCATACCTATATCAACCGCTTAACCCTTCTATTTTAAGATTAGTAAAAATGGTTATTGATGGTGCTCACAAACATGGTAAATGGGCTGGTATGTGCGGTGAAATGGCCGGAGATAGACGCGCATTGCCTTTACTTTTAGGTTTAGGTTTAGATGAATTTAGTATGTCTGCTGGTAATGTTTTAGCTTCAAGAGAATTGGTATCTCAATTAAGCAAAAAAGACATGGAAGAATTGGCTGCTCAAGCTATTGAATTAGATAGTGAAGAACAAGTTATTAAACTTTTAAATAAAGCTTTAAAATTAAAATAA", "aa": "MKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK", "aa_hexdigest": "421008abf5a5322aa455ec99bbb5cc7b", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FF43100", "query_cov": 0.9920318725099602, "subject_cov": 0.8861209964412812, "identity": 0.759, "score": 377.0, "evalue": 9.49e-130, "valid": false, "product": "phosphoenolpyruvate--protein phosphotransferase", "uniref50_id": "UniRef50_A0A938YW30" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 574971, "stop": 576026, "observations": { "mutation": [ 575777 ], "stop": [ 575777 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBF2", "query_cov": 1.0, "subject_cov": 0.42327150084317033, "identity": 1.0, "score": 483.0, "evalue": 3.01e-167, "gene_start": 1, "gene_end": 251, "reference_start": 343, "reference_end": 593, "reference_sequence": "MKSHKRLYVYIIKLHTYLNKMEVKMTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDPWALETITGRIKDNKETAEYATKAFYEEMATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEHWPTEEEQFAAYKEVVERMKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK" }, "paralog": false, "identity": 0.9971509971509972, "score": 687.0, "evalue": 1.32e-249, "description": "Internal stop codon at 575777. Nonsense mutation around 575777.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBF2", "product": "Phosphoenolpyruvate-protein phosphotransferase", "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBF2", "UniRef:UniRef50_W6A961" ] }, "pscc": { "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "EFPKPENHBP_1066", "locus": "GCA000186005_01038" }, { "type": "cds", "sequence": "contig_1", "start": 575775, "stop": 576365, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00391.29", "PFAM:PF02896.24" ], "nt": "ATGGCAACAATGTTTTCAAATATGGACGATGCCTACATGAAAGAAAGAGCTGCTGATGTTAAAGATGTTATGAAAAAACTTCTTTACATTTTAAATGACATTGAAGAACCTGATTTAACTGCTATTGATAAAGAAGTAATTATTGTGGCTTATGATTTAAGTCCTTCTCAAACTGTTCAATTAAATAAAAAATATGTTAAAGGTTTTGCAACCAACATTGGTGGACCAACAAGTCATACAGCAATTATGGCTAGAAGCATGAACATTCCTTCAGTAGTTGGAACAAACAATGTTTTAGAATCAGTTAAAAATGGTCAAACAATTATTATTGATGGAATCAAAGGCGAAGTAATTGTTGATCCTACAAAAGAAGAATCAGAACACTATAAAAAAGAAGAAGCTAAATACATTAAATATTTAGAAACAATTGCTAAATACAAAGGTAAAAAATCAATTACTAAAGATAAACATCATGTTGAATTAGCTGCAAATATTGGACGTCCAAAAGATGTTGATGCAGTTATTGACAATGATGCTGAAGCAATTGGGTTATTCCGTTCAGAATTCTTATACATGGATAATGAACACTGA", "aa": "MATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEH", "aa_hexdigest": "61080fa331e7a2447a8542b4409ab0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00391.29", "name": "PEP-utilizers", "length": 73, "aa_cov": 0.37244897959183676, "hmm_cov": 0.9726027397260274, "evalue": 1.623327138761744e-26, "score": 88.05028533935547, "start": 43, "stop": 115 }, { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 52, "aa_cov": 0.2653061224489796, "hmm_cov": 1.0, "evalue": 3.49836067873323e-13, "score": 45.6678466796875, "start": 144, "stop": 195 } ], "seq_stats": { "molecular_weight": 21956.865300000012, "isoelectric_point": 5.436558723449706 }, "id": "EFPKPENHBP_1067", "locus": "GCA000186005_01039" }, { "type": "cds", "sequence": "contig_1", "start": 576444, "stop": 576680, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAAAGGAATCGGAGCAAGTCGTGGAATCGCGGTTGCAAAAGTTTTTAAAATTGAAGAACTACCAGTCAATATTACTGAAAAAGCTAAGGATGCAGATCATGAATTAAAACTATATAATGATGCTGTTAAAAAAGCTGGTGAAAAAGTTGAAGCTACTAAAAAATTAGCTAAAACTCCTGAACAAGCTGCTATTTTTGATGCGCATTTACAAATTATTAATGACCCTTGA", "aa": "MTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDP", "aa_hexdigest": "daf661ae6e2f783cd7f62229f659d7f7", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8483.747499999998, "isoelectric_point": 7.969017601013183 }, "id": "EFPKPENHBP_1068", "locus": "GCA000186005_01040" }, { "type": "cds", "sequence": "contig_1", "start": 576922, "stop": 577065, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTAGCTGCTGATGTTCATGCGCCTAAGGATTTAAGAGCAAATATTCAAGCTAAAAACTTAGATGAATTTCATGTAACATTTAAAACAAAACCTAGTGATAAGATGTATTTAGCACCTGAAAAAAGAGTCAAAATCTGA", "aa": "MLLAADVHAPKDLRANIQAKNLDEFHVTFKTKPSDKMYLAPEKRVKI", "aa_hexdigest": "2456438a79a605ac355e2e761026d5e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.290500000001, "isoelectric_point": 9.52199649810791 }, "id": "EFPKPENHBP_1069", "locus": "GCA000186005_01041" }, { "type": "cds", "sequence": "contig_1", "start": 577108, "stop": 577266, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATCTTTTTGAAGGCCAAGATTCAGAATTTGGTAAATGTAATGGTAAATTAACTGTATCTGAAAATATTGCTGATGCTGGTGGAATTAGATGTGCATTAGAAGCTAGTCAAAAAGAATTAAATCATTCATCAAAAGAATTCTTTACAAACTGA", "aa": "MVDLFEGQDSEFGKCNGKLTVSENIADAGGIRCALEASQKELNHSSKEFFTN", "aa_hexdigest": "b883c3b01f53be6c7dc51cb7daaa4681", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5667.191499999998, "isoelectric_point": 4.638823509216309 }, "id": "EFPKPENHBP_1070", "locus": "GCA000186005_01042" }, { "type": "cds", "sequence": "contig_1", "start": 577312, "stop": 577521, "strand": "-", "frame": 1, "gene": null, "product": "M13-type metalloendopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "nt": "ATGTCACCTGCTGAAGTGAATGCTTACTATAATCCTTTTATGAATCATATAGTTTTCCCTGCTGGGATTTTAGCAAAACCATTCTACTCATTAACCCAAAGTTCTTCAGCTAACTATGGTGGAATTGGAGCTGTTATTGCTCACGAAATTTCACATGCTTTTGATAATAATGGAGCTAAATTTGATGAAAAAGGTAATTTAAATTCATGA", "aa": "MSPAEVNAYYNPFMNHIVFPAGILAKPFYSLTQSSSANYGGIGAVIAHEISHAFDNNGAKFDEKGNLNS", "aa_hexdigest": "4d0f0ef07347504266be0d5db2a78593", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025523856", "query_cov": 0.927536231884058, "subject_cov": 0.8205128205128205, "identity": 0.516, "score": 72.4, "evalue": 2.42e-15, "valid": false, "product": "M13-type metalloendopeptidase", "uniref50_id": "UniRef50_UPI0025523856" }, "pscc": { "uniref50_id": "UniRef50_UPI0025523856", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "product": "M13-type metalloendopeptidase" }, "genes": [], "id": "EFPKPENHBP_1071", "locus": "GCA000186005_01043" }, { "type": "cds", "sequence": "contig_1", "start": 577525, "stop": 577644, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAACTCCATATAAACATGAAGGTAATTTAGTAGTTAATGCATTAAATCTTAATGCAATTGAAAGCGAATATAACTTTAGTCAATACAAACAACCAATTAATAAAAATTTATGA", "aa": "MVITPYKHEGNLVVNALNLNAIESEYNFSQYKQPINKNL", "aa_hexdigest": "f06ea9320b2c4b85cf7627aa8624f18b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4508.071700000001, "isoelectric_point": 6.519455146789551 }, "id": "EFPKPENHBP_1072", "locus": "GCA000186005_01044" }, { "type": "cds", "sequence": "contig_1", "start": 577741, "stop": 578010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTAGAAACTTAATTAGATTTTCAAATCTTTTGGACAATAAAACTAGAATTACTGCAGGAGAATTTAACCGTGCAGTAAGTGGAATTGATAAAGCTTCAAGTGCAGCTAAAAGTGCTTTCTACTTAGCTTATTCATACTTTAAAATTCCTTTTGGTTTAAGATTTGCACTTGAAAATTTTGGTCCTGAAGCTAAAAGAAATGTTGAATATATGGTTCAAAAAATGATCAAAATTTATCAAGACCGTTTAAGAAACAACAGCTGA", "aa": "MYLRNLIRFSNLLDNKTRITAGEFNRAVSGIDKASSAAKSAFYLAYSYFKIPFGLRFALENFGPEAKRNVEYMVQKMIKIYQDRLRNNS", "aa_hexdigest": "a133bad68f540abfca27b32ddb24981f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10353.833199999994, "isoelectric_point": 10.05502109527588 }, "id": "EFPKPENHBP_1073", "locus": "GCA000186005_01045" }, { "type": "cds", "sequence": "contig_1", "start": 578011, "stop": 578478, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTGATTTTGAAGTAATGCAAGATTTTAAAAATACCGAATTACAAACTTTAGGTTTTAGTGGACCAAGTTTAATTTTGCCAGAAAAATCATATTATGATGACAATCATCCAGCTAAACAACCGCTTTTAGAAGTATTTAAAAACATGTCAAGAAAATTATTAGCTGCTTATTTTAAAGATAAAAATAAAATTGAAGATATTATTTCTAATACATTAGAATTTGATAACTCATTAGTTAAATACACAATGAGTGCAGTTGAAAGTGCTGACTATCCCTCTTTATATAATCCTTTTACAATTGATGAAATTAATGAACATTCAAAAGTTTTTGATCTTAAAGAAATAGCAGAAAGATTAATTAAACAAGAAGTTAGCGAAAAAGTTGTTGTAATTTATCCTAAATTTATGAAAGGTTTAAATAGCTTATATAACTCAAGAACATTTGAAACATTTAAATGCTGA", "aa": "MPFDFEVMQDFKNTELQTLGFSGPSLILPEKSYYDDNHPAKQPLLEVFKNMSRKLLAAYFKDKNKIEDIISNTLEFDNSLVKYTMSAVESADYPSLYNPFTIDEINEHSKVFDLKEIAERLIKQEVSEKVVVIYPKFMKGLNSLYNSRTFETFKC", "aa_hexdigest": "9b945939e30db9d4665034f7a33c910f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18076.451199999996, "isoelectric_point": 5.122978019714354 }, "id": "EFPKPENHBP_1074", "locus": "GCA000186005_01046" }, { "type": "cds", "sequence": "contig_1", "start": 578542, "stop": 578694, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGATTTAGTTAGAACTCGTGATGCAGGTGAATTAGAAGTTGGAGCAATTTTAAACTTCACAAATCTTTATTCAATGGCTAGTGATTTTACTTTTAGAAATAAATCTAAAGTTAAACCACTTAAACCTTACTTAAATAAAATTTGA", "aa": "MASDLVRTRDAGELEVGAILNFTNLYSMASDFTFRNKSKVKPLKPYLNKI", "aa_hexdigest": "0fb247e5823063ec4eb8a7e71f994ff8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5664.5136, "isoelectric_point": 9.599229621887208 }, "id": "EFPKPENHBP_1075", "locus": "GCA000186005_01047" }, { "type": "cds", "sequence": "contig_1", "start": 578902, "stop": 579330, "strand": "-", "frame": 1, "gene": null, "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "EC:2.1.1.193", "KEGG:K09761", "SO:0001217", "UniRef:UniRef50_A0A449B7G8", "UniRef:UniRef90_C4XEZ0" ], "nt": "TTGCAAAAAGCTACTGAATTAGGAGTTACTACAATTATTCCTTTTGCCTCTAAATATACTGATGCAAAAATCTTAAAATATGAATTCAACAGTAAGCAAAATAGGTTTAATGAAATCATTAAAAATGCAGCTGAACAAAGCTTTAGAAACATCATTCCTTATTTAACTAAAATTATGAATTTAGAAGAAATTATTAAGACTTATCCTACTATGCAAAAGTACTTAGCTTATGAAAATCAAGACCAAAAAAATAACATTATCACTTATCCAACTAGTTCAATTTTAATAGTTGGACCTGAAGGTGGTTTTAGTCCTGAAGAAATAGAATTAGCTAAAGAAAACAATATTAAAATTACTTCATTAGGCAAAACAATCTTACGTGCTGAAACAGCTTGTTTGTATATGCTAGCTAATATCAAAGAAAAATAA", "aa": "MQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK", "aa_hexdigest": "2246d4ae69939870fd27bd08e12df65f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 578902, "stop": 579600, "observations": { "mutation": [ 579336 ], "stop": [ 579336 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEZ0", "query_cov": 1.0, "subject_cov": 0.6120689655172413, "identity": 0.993, "score": 280.0, "evalue": 3.54e-94, "gene_start": 1, "gene_end": 142, "reference_start": 91, "reference_end": 232, "reference_sequence": "MNERNKMHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFEWILQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK" }, "paralog": false, "identity": 0.9913793103448276, "score": 456.0, "evalue": 3.89e-166, "description": "Internal stop codon at 579336. Nonsense mutation around 579336.", "psc": { "uniref90_id": "UniRef90_C4XEZ0", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A449B7G8", "kegg_orthology_id": "K09761", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEZ0", "EC:2.1.1.193", "UniRef:UniRef50_A0A449B7G8", "KEGG:K09761" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7G8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7G8" ], "product": "Ribosomal RNA small subunit methyltransferase E" } }, "id": "EFPKPENHBP_1076", "locus": "GCA000186005_01048" }, { "type": "cds", "sequence": "contig_1", "start": 579334, "stop": 579582, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGATTTTTTGTTACTAAAAAAAGTGATCAAGATCATTTTGAACTATCAAAAGAGATATTAAATCACATCAAAGTTGCACGTGTTGAAAAAGATGAATTTTTATGTAACTATCAAGACATTTTTTATAAGTGCAAATTAGAAAATAATAAAGCTTTAATTATCGAAAAATTAGATATAAATCATGAATTTAATAAGCCAATTATTCTTGCTGCACCAATTATTAAAATTAAAAGATTCGAATGA", "aa": "MHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFE", "aa_hexdigest": "da417bb2bf34f539d5efe3f686e7eaf7", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9904.495299999988, "isoelectric_point": 8.566639518737794 }, "id": "EFPKPENHBP_1077", "locus": "GCA000186005_01049" }, { "type": "cds", "sequence": "contig_1", "start": 579747, "stop": 580895, "strand": "-", "frame": 2, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "GO:0000910", "GO:0003924", "GO:0005525", "KEGG:K03531", "RefSeq:WP_013354660.1", "SO:0001217", "UniParc:UPI0001E33107", "UniRef:UniRef100_C4XEZ1", "UniRef:UniRef50_C4XEZ1", "UniRef:UniRef90_C4XEZ1" ], "nt": "ATGGACTTAAATGCTGAAGATTTGGAAGTTAAACTAAAAGTCATTGGTGTTGGTGGTGCAGGTAATAACGCCATTAACTTGATGCTTGATGAAAACTTGCCAAATGTTGAATTACTAGTTGCTAACTCAGATCGTCAAGATTTGGTTAAAAGCTTATGTCCAAATAAAATACTTTTAGGTGATTCTACCAGAGGCTTTGGTGCTGGTGGGGACCCTAAAGTAGGACGTGAGTGCGCACTAGAAAGCATTAAAGAAATTCAAAAATCATTAGAAAACACAGATATTGTTATTATTTCAGCTGGCTTAGGCGGCGGAACAGGAACTGGAGCAGCACCAGTGATTGCCGAAGCTGCCAAAAAGATGGGCATTTTGACAGTGGCAGTAGTAACCACTCCTTTTGAATTAATTGAAGGAAAACATAAAAGCTTAATTGCTCAAGAAGGCTTGAAAAAATTAAGTGAAGTTGTTGATAGTTATATTGTAATTTCAAACCAAAAATTGGTTGAAAATTACAGAAACTTACCAGTTCAAGAAGCTTTTAAAGTTTCTAACTATACATTGAAAAATTCAATCAAAATAATTCGTGATATTATCTTTGAAACTGGGTTTATTAATTTAGATTTCAATGATTTACGTCAAGTTTTATTAGATGGAAAAGAAACAATTATAGGAATTGGTAATGGCTTTGGCAAAGACCGAGCTATTAAAGCAGTTGATGATGCTTTAATGACTCCACTTTTTCAAAGTGAAATTAAAAATTGTCAAAAAGTTGCTATTTTATTTCAGTGTGATAAAAGAGCAAGTTTAGATGACATTGAAACAGCAAAAAATCGAATTGATGAGTATTTAGCAAATAACCTTGAAGCTCAAACTTTTATCGGACTTCAATATATCGATACTCAAGATCGTGAAGAAATATTCAGAATTAGTATTATTGCTTCAAATCTTAATGCAAATGTTTCAACAAATACTTATGCGGTACCAAAATTATTGCAAAGAAATGTTAGTGAACATTATGAGCAAAATATGCAAAATACATATACACAAATTAATGGATCACATCATACTCGTATAATGAGTTATACTGCTCAAAACTATGAAGATGAAAATGAAGATGTAATGCCAAATTTCTTTGATGAAAATCATTAA", "aa": "MDLNAEDLEVKLKVIGVGGAGNNAINLMLDENLPNVELLVANSDRQDLVKSLCPNKILLGDSTRGFGAGGDPKVGRECALESIKEIQKSLENTDIVIISAGLGGGTGTGAAPVIAEAAKKMGILTVAVVTTPFELIEGKHKSLIAQEGLKKLSEVVDSYIVISNQKLVENYRNLPVQEAFKVSNYTLKNSIKIIRDIIFETGFINLDFNDLRQVLLDGKETIIGIGNGFGKDRAIKAVDDALMTPLFQSEIKNCQKVAILFQCDKRASLDDIETAKNRIDEYLANNLEAQTFIGLQYIDTQDREEIFRISIIASNLNANVSTNTYAVPKLLQRNVSEHYEQNMQNTYTQINGSHHTRIMSYTAQNYEDENEDVMPNFFDENH", "aa_hexdigest": "61bfc8f8ae42dae9f9463bea5f770824", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33107", "ncbi_nrp_id": "WP_013354660.1", "uniref100_id": "UniRef100_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33107", "RefSeq:WP_013354660.1", "UniRef:UniRef100_C4XEZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ1", "uniref90_id": "UniRef90_C4XEZ1" }, "psc": { "uniref90_id": "UniRef90_C4XEZ1", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_C4XEZ1", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ1" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "EFPKPENHBP_1078", "locus": "GCA000186005_01050" }, { "type": "cds", "sequence": "contig_1", "start": 580980, "stop": 581276, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCTTTATTTGACCAAGCTATTCATAAAGTTAAAGCGTTTTTAGACCAAAATAATCAAAAAGTTGATGAGTTGGTTTTAAACTGCAAAAACTCAGAAATCTTTATGGATCGTTTTAACAAATTATATCCCGAAATGAATGTTGCAATTCTAGACGATAACGTCTCAACTTTATTCAATATACCTAATAGCCTTTTAGGCATTATTAATTTACTAAATCCTGAAAAAATCAATTGTGAAGACATGGTTAATACTTTGAATAATTTACCAGAATACAAACCAAAAAGTTTTTGA", "aa": "MISLFDQAIHKVKAFLDQNNQKVDELVLNCKNSEIFMDRFNKLYPEMNVAILDDNVSTLFNIPNSLLGIINLLNPEKINCEDMVNTLNNLPEYKPKSF", "aa_hexdigest": "bd784c4c0456baab6732eb2f6dcc214c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11317.950999999986, "isoelectric_point": 4.780182075500488 }, "id": "EFPKPENHBP_1079", "locus": "GCA000186005_01051" }, { "type": "cds", "sequence": "contig_1", "start": 581331, "stop": 581462, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAAAACATTTTTCTATGAAAAATCTAACATTGACTTTAAAAACTTTGTTAATTATTTATCAAAAGAATTAAATAAAAGTAAAGAAGATACAATTTTGTTACTTGAAGCTGTGGTAGCTAATTGA", "aa": "MNAKTFFYEKSNIDFKNFVNYLSKELNKSKEDTILLLEAVVAN", "aa_hexdigest": "56f21b868eee47f2cdce876dd7497f23", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5030.702700000001, "isoelectric_point": 6.089979743957519 }, "id": "EFPKPENHBP_1080", "locus": "GCA000186005_01052" }, { "type": "cds", "sequence": "contig_1", "start": 581805, "stop": 582113, "strand": "-", "frame": 2, "gene": null, "product": "MAG3720 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D", "UniRef:UniRef90_UPI000FEECA7D" ], "nt": "ATGAAAAAAATTTATGTAAATTTCTTTCTTAAAAAAACTAGACTTGATGTGACAGTAGTGGAAGATATTAATTCTAAATATTTTCCAGTTTTTCAAACATATCTAGAAACCAAAAACTTTGAAGCGAATAAGATTAAAGAATTTATTACGAATTTCAAAAAATATTTGAAATCTAAAGTTGCTACCAAGAAAAATGATGTAGTTAAATATTCTTTTATAGTTAAAGATAGTTTATACAAAAGTTTAAAATTTGTTAACCAAACTTTGACTTTTGATGTTCCAAGTAATACAGTTGATCAACAAGTATGA", "aa": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQV", "aa_hexdigest": "36e174daa2dba9331720acc57107e6a9", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 581650, "stop": 582113, "observations": { "deletion": [ 581699 ], "mutation": [ 581807 ], "stop": [ 581807 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECA7D", "query_cov": 1.0, "subject_cov": 0.6623376623376623, "identity": 1.0, "score": 199.0, "evalue": 6.97e-64, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQVWKEVKNKLEEQKESSTYELINSNIYLYETINNDVVKKLQYFSLKQTSSKIKY" }, "paralog": false, "identity": 0.9935064935064936, "score": 294.0, "evalue": 8.76e-104, "description": "Internal stop codon at 581807. Frameshift due to deletion around 581699. Nonsense mutation around 581807.", "psc": { "uniref90_id": "UniRef90_UPI000FEECA7D", "product": "MAG3720 family protein", "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECA7D", "UniRef:UniRef50_UPI000FEECA7D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D" ], "product": "MAG3720 family protein" } }, "id": "EFPKPENHBP_1081", "locus": "GCA000186005_01053" }, { "type": "cds", "sequence": "contig_1", "start": 582163, "stop": 583125, "strand": "-", "frame": 1, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "EC:2.1.1.199", "GO:0016434", "KEGG:K03438", "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_A0A3P8MEW3" ], "nt": "ATGACAGTGACATATCTTCAGCAGCACAAAAAATTCTTGAAAGAAGTGGTAAATAAAATGGAGAATCAACACTTTTCTGTTTTACTGCAAGAGTCTATTGATGCCCTTAATATAAAACCAGATGGTATTTATGTTGATTTAACTTTGGGCATGGGTGGACATTCTAGCGAGATTTTAAAGAGACTTGACACTGGTCGTTTGATTGCTTTTGATAAGGACACTTTTGCGATAGAAGAAAGTCGCAAACGTCTCAGCCAAATAAAATCAACTAATTTTCAACTAATTCATTCTGACTTCAAAAATATAAAAAGTGAATTAGATAAATTGAACATTAGCCAAGTAGATGGAATCATTGCTGATTTAGGTATTAGCAGTCCACAAGTTGATAATGATGAACGAGGTTTCTCTTATAACAAAAATGCACGTCTTGACATGCGCATGGATCAAAGTCAAAAGCTTGATGCCTACTATGTAGTTAATAATTATAGTGAAAGTCAATTGGAACAATTATTAAGAAACTACGCTGAGGTAAAACTTGCTCGTCAGGTTGCGAAGGCCATCATTAGTAAGCGGCCAATAACTACCACTTTGGAATTAGCTCAAGTAGTGCGAGATGCTTATCCTGCAAAATTAGTGAAACTAAAAAATCCTAATAAAGCAGTGTTCCAAGCTATCCGTATTGAAGTTAATAATGAATTTGATTCCCTTAAGCAAATGTTACAAGATGCTATTGAACTATTAAAACCTAATACTTCTTTAGCCGTTATAACTTTTCATTCATTGGAAGACAGCATTGTTAAACATTTTTTTGGTTCACTAATTAAAAGTAAATTACCAGCAAAAATGCCAATTAATGAAGAGAAGCAATATAAAGTAAAAGTATTTCTTCCAACCAATAATGAGTTAGAACTTAATAAACGCTCTCGTAGCGCCAAGTTAAGAGTTTTGAAAAAATGCTTTTAA", "aa": "MTVTYLQQHKKFLKEVVNKMENQHFSVLLQESIDALNIKPDGIYVDLTLGMGGHSSEILKRLDTGRLIAFDKDTFAIEESRKRLSQIKSTNFQLIHSDFKNIKSELDKLNISQVDGIIADLGISSPQVDNDERGFSYNKNARLDMRMDQSQKLDAYYVVNNYSESQLEQLLRNYAEVKLARQVAKAIISKRPITTTLELAQVVRDAYPAKLVKLKNPNKAVFQAIRIEVNNEFDSLKQMLQDAIELLKPNTSLAVITFHSLEDSIVKHFFGSLIKSKLPAKMPINEEKQYKVKVFLPTNNELELNKRSRSAKLRVLKKCF", "aa_hexdigest": "ca69d16297885b96282fb4baac62a1bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C25A", "uniref100_id": "UniRef100_C4XEZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ3", "uniref90_id": "UniRef90_A0A3P8MEW3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEW3", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "kegg_orthology_id": "K03438", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "EFPKPENHBP_1082", "locus": "GCA000186005_01054" }, { "type": "cds", "sequence": "contig_1", "start": 583163, "stop": 583291, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATACTCAAGAAATTGAACTTGACAACCAAGGACGTTTCGTTGTTCCAAAACAATTCCTCGACAAAGCTGCTATCCAAAAAGAAGCCATTTTTGTAGCCTCAGGTAATCTTGTAGAACTTTGA", "aa": "MSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVEL", "aa_hexdigest": "e6c6179a47da3362b311faa7d14a7294", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4692.305799999999, "isoelectric_point": 4.655875205993653 }, "id": "EFPKPENHBP_1083", "locus": "GCA000186005_01055" }, { "type": "cds", "sequence": "contig_1", "start": 583292, "stop": 583504, "strand": "-", "frame": 3, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44", "UniRef:UniRef90_A0A3P8KXP2" ], "nt": "ATGTTTGGACAATACAAAAGAAACATTGACGACAAAAAAAGATTAGCTTTACCATCTAAATTGCGTGACGATTTAGGAAGCAAAATTTATTTAACTGTCGGACTTGAAGGCATTGTTGAAATTAGAAGCGAAAAAGAATTCAATAAATTCGTTGAAGTTCTAAATCAACAAAACTCTTTTGATACAAATGCTCGTTTTGTTAGAAGATTTTGA", "aa": "MFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRF", "aa_hexdigest": "fc1bcb8076e3e37f9a1362ad29c21bbf", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "mraZ" ], "truncated": "5-prime", "pseudogene": { "start": 583070, "stop": 583534, "observations": { "mutation": [ 583165, 583294 ], "stop": [ 583165, 583294 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KXP2", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 1.0, "score": 142.0, "evalue": 6.29e-42, "gene_start": 1, "gene_end": 70, "reference_start": 11, "reference_end": 80, "reference_sequence": "MVVDSGRKWKMFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRFWLSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVELWGSEKFDQYCQNFNDSDISSAAQKILERSGK" }, "paralog": false, "identity": 0.9805194805194806, "score": 295.0, "evalue": 6.67e-105, "description": "Internal stop codon at 583165, 583294. Nonsense mutation around 583165, 583294.", "psc": { "uniref90_id": "UniRef90_A0A3P8KXP2", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KXP2", "UniRef:UniRef50_F9UK44" ] }, "pscc": { "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44" ], "product": "Transcriptional regulator MraZ" } }, "id": "EFPKPENHBP_1084", "locus": "GCA000186005_01056" }, { "type": "cds", "sequence": "contig_1", "start": 583748, "stop": 584425, "strand": "-", "frame": 3, "gene": null, "product": "Potassium uptake protein A", "db_xrefs": [ "RefSeq:WP_013526894.1", "SO:0001217", "UniParc:UPI0001F5ABF9", "UniRef:UniRef100_A0AB32XC29", "UniRef:UniRef50_C4XEZ5", "UniRef:UniRef90_C4XEZ5" ], "nt": "ATGCCTCGTAAAAGAAAAAATGAATATATCTGTGTTATTGGAACAGGGCGTTTTGGTAGTGCGGTTGTAAGCCAACTTGCTAAAATGAATTGTCCTTTATTATTAATAGATAAAGATGAAGAAGTTTTAAAATCATATGCTGATTTAGCACAAAAAGTTGTTATTGCTGATGCAGGTGATATGAAAGCTTTAAGAGCACTTAATCTTCAAGAAATTGGAACTGTTGTTGTTGCTGTTCCTGATAATATTGAAATTGTGGCTGCTTTACTCGAATTAAAAGTTAATAATATTATTGCTCGTGCAACAAGTAAAAGACATGCACGTGTATTAAAACAAATTGGAGTTAATGTTATTGTTAGACCTGAATATGAAGCCGGTGTTCGTACTGCTCTTTTAGCAGCTAATCCAAATTTTAGCCGTTATTCTCAAACATTACAAGAAATTGGCGATGGCTTCGTTATGGGTACTACTATTTTAAATAATCCAAAATTAATCAATAAACCAATCAAAGAATTAAAATTCTTTAACTTAGGAATTTCAGTTGTTTTAATTAAGAGAGGAACTGAAAGTATTTTACCTACTGGGGTAACTGTTTTACAAAAAAGTGATCTTATTACAATTCTAGGTAAGGTTGAAGATGTAACCAATGCTTTTGGTTTCTTAAATGTCGAAGACTAG", "aa": "MPRKRKNEYICVIGTGRFGSAVVSQLAKMNCPLLLIDKDEEVLKSYADLAQKVVIADAGDMKALRALNLQEIGTVVVAVPDNIEIVAALLELKVNNIIARATSKRHARVLKQIGVNVIVRPEYEAGVRTALLAANPNFSRYSQTLQEIGDGFVMGTTILNNPKLINKPIKELKFFNLGISVVLIKRGTESILPTGVTVLQKSDLITILGKVEDVTNAFGFLNVED", "aa_hexdigest": "a6279cca7ed9f91db05156a5005f1959", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F5ABF9", "ncbi_nrp_id": "WP_013526894.1", "uniref100_id": "UniRef100_A0AB32XC29", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5ABF9", "RefSeq:WP_013526894.1", "UniRef:UniRef100_A0AB32XC29" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC29", "uniref90_id": "UniRef90_C4XEZ5" }, "psc": { "uniref90_id": "UniRef90_C4XEZ5", "product": "Potassium uptake protein A", "uniref50_id": "UniRef50_C4XEZ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ5" ], "product": "Potassium uptake protein A" }, "genes": [], "id": "EFPKPENHBP_1085", "locus": "GCA000186005_01057" }, { "type": "cds", "sequence": "contig_1", "start": 584881, "stop": 585090, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGA", "aa": "MFGQAIIAILIFLGGLGIFALRIFLINLIFFKRRNSLSELEVVSHERGSGDSGQTKKLIMDSIGTLLII", "aa_hexdigest": "72e2093f0a50a4cc2a10b6822ff0e2da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7607.031999999997, "isoelectric_point": 9.687744712829591 }, "id": "EFPKPENHBP_1086", "locus": "GCA000186005_01058" }, { "type": "cds", "sequence": "contig_1", "start": 585280, "stop": 585423, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGA", "aa": "MPYYHNIGLQIIFLTLLIIGGLGYPVIHDILNFFRFLIKYKGKRRYQ", "aa_hexdigest": "b42de4efa5623b2c760ac6ac8621978d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.7618999999995, "isoelectric_point": 9.993905067443851 }, "id": "EFPKPENHBP_1087", "locus": "GCA000186005_01059" }, { "type": "cds", "sequence": "contig_1", "start": 585676, "stop": 586086, "strand": "+", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6", "UniRef:UniRef90_UPI0021AD00B6" ], "nt": "ATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGA", "aa": "MFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILV", "aa_hexdigest": "83415cf343d9ab6d60be54f0641ea146", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 585625, "stop": 586149, "observations": { "mutation": [ 586084 ], "stop": [ 586084 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021AD00B6", "query_cov": 1.0, "subject_cov": 0.7816091954022989, "identity": 1.0, "score": 256.0, "evalue": 7.61e-86, "gene_start": 1, "gene_end": 136, "reference_start": 18, "reference_end": 153, "reference_sequence": "MNFSKLTSTSLIICSILMFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILVWGKKKNYSNKYEYISEPVAIG" }, "paralog": false, "identity": 0.9885057471264368, "score": 332.0, "evalue": 7.68e-118, "description": "Internal stop codon at 586084. Nonsense mutation around 586084.", "psc": { "uniref90_id": "UniRef90_UPI0021AD00B6", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021AD00B6", "UniRef:UniRef50_UPI0021AD00B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6" ], "product": "potassium transporter TrkG" } }, "id": "EFPKPENHBP_1088", "locus": "GCA000186005_01060" }, { "type": "cds", "sequence": "contig_1", "start": 586650, "stop": 587435, "strand": "+", "frame": 3, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00", "UniRef:UniRef90_C4XF00" ], "nt": "ATGAAATTAAATTTATTTGCGTCTGAATACAAATCATTGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGA", "aa": "MKLNLFASEYKSLTNAEQKIIDYINENPNEFAENSIEKNAVETFASTAVLSRIYKKLGFSSYKDMQFYVKYNYLSNLKIPKELLDNSIYKIAKTYMEAINLTSKYLDIKEIDYICEEINKSKLVYCFGIGSSSISAKELSLNIEKFDYYSVFHNDFHNFLISLYSHELKENKPPVILFSKSGKTNEILFLCETLLEHKFPFLLITANRSLKNKYPHVLLHETIEQKERYHALSSKIVQQYIADIIADHLISNSPNKLNRKN", "aa_hexdigest": "49c50d289a29db5aba707230865bf656", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF00", "query_cov": 1.0, "subject_cov": 0.9456521739130435, "identity": 1.0, "score": 508.0, "evalue": 1.93e-181, "valid": true, "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XF00" }, "pscc": { "uniref50_id": "UniRef50_C4XF00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00" ], "product": "RpiR family transcriptional regulator" }, "genes": [ "rpiR" ], "id": "EFPKPENHBP_1089", "locus": "GCA000186005_01061" }, { "type": "cds", "sequence": "contig_1", "start": 587510, "stop": 588205, "strand": "-", "frame": 3, "gene": null, "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "db_xrefs": [ "RefSeq:WP_013354668.1", "SO:0001217", "UniParc:UPI0001E3310F", "UniRef:UniRef100_A0AB32XC18", "UniRef:UniRef50_A0A097SSE0", "UniRef:UniRef90_A0AB32XC18" ], "nt": "ATGAGTCTTAAAAATAATTTGTTTATAGTTTCTTGTCAAGCATTGGAAAATGAGCCAATGTATGGTCCTGGTGTAATCGAAAGAATGGTTAAGTCTGTTTTATTAGGTGGTGCTCAAGGCATAAGAATTAGCCAAAGAGAAAATATTGAACCAGTCTTAAGAATAGTACCTAAAGACATTCCTGTTATTGGTATTACTAAAAAAATGTCTAAAGATTCTGAAGTATTTATTACTCCTGATTTAGATGATTTAAAATTCTTAATTGAAAGTAAATGTCCAATAATTGCAATAGATGCAACTTTAAGAAAAAGACCAAAAGAAAGTTTGGAAGACTTAGTTCAATATTTCAAGAAAAATAATCATAATCAGTTATTAATGGCAGATTGCTCTTCAATTGAAGATGTTGAAAATGCTTTTAAATTAGATTTTGATATTGTTTCAACTACTCTTAGAGGCTACACAAAAGACACATTAAATAAACCTAATAGTTACAATGATTTTGAATTTCTAAAAGAAGTAGTTGAATTAAATAAAAAGTACAAAAAATACATAATTGCTGAAGGCGGATTTAATACACCTGAAATGGTCCAAAAAGCCTATAAAATAGGTGCAAATGGTGTAGTTGTTGGCTCAGCAATTACTAGACCAACTTTTATAACAAAACAATTTGTTGACTATTTAAGCAATAAAAAATAA", "aa": "MSLKNNLFIVSCQALENEPMYGPGVIERMVKSVLLGGAQGIRISQRENIEPVLRIVPKDIPVIGITKKMSKDSEVFITPDLDDLKFLIESKCPIIAIDATLRKRPKESLEDLVQYFKKNNHNQLLMADCSSIEDVENAFKLDFDIVSTTLRGYTKDTLNKPNSYNDFEFLKEVVELNKKYKKYIIAEGGFNTPEMVQKAYKIGANGVVVGSAITRPTFITKQFVDYLSNKK", "aa_hexdigest": "982de2b82f08ad3af98b8d5627f9c901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3310F", "ncbi_nrp_id": "WP_013354668.1", "uniref100_id": "UniRef100_A0AB32XC18", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3310F", "RefSeq:WP_013354668.1", "UniRef:UniRef100_A0AB32XC18" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC18", "uniref90_id": "UniRef90_A0AB32XC18" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC18", "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "uniref50_id": "UniRef50_A0A097SSE0" }, "pscc": { "uniref50_id": "UniRef50_A0A097SSE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097SSE0" ], "product": "Putative N-acetylmannosamine-6-phosphate 2-epimerase" }, "genes": [], "id": "EFPKPENHBP_1090", "locus": "GCA000186005_01062" }, { "type": "cds", "sequence": "contig_1", "start": 588732, "stop": 588935, "strand": "-", "frame": 2, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "nt": "ATGGGTATTGGTTTAGCTATGATTCTTTTAGCTGAAAAAGAAAATCGTAAAAATGTAGCGATAACAATAGGTTCAGCAATGGGTGTTGCATTCTTAACAGGTATTACAGAACCTCTTGAATTTACATTCTTGTTTATTGCTCCTCAACTTTATTATGCTATTTATGTACCACTTAGTGGTTTAGCATATGCATTAATGGAATGA", "aa": "MGIGLAMILLAEKENRKNVAITIGSAMGVAFLTGITEPLEFTFLFIAPQLYYAIYVPLSGLAYALME", "aa_hexdigest": "27feea9e1a1b1384811f86f00fb7d837", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00168119FF", "query_cov": 0.9253731343283582, "subject_cov": 0.8611111111111112, "identity": 0.5, "score": 50.8, "evalue": 6.31e-07, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI00168119FF" }, "pscc": { "uniref50_id": "UniRef50_UPI00168119FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "EFPKPENHBP_1091", "locus": "GCA000186005_01063" }, { "type": "cds", "sequence": "contig_1", "start": 589062, "stop": 589316, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGATCACCAATAGGTTTAGATGGATTTATTTATGGTATATTAGGCCGTGCTTTAATGCCTTTTGGTTTACACCACATTCCAATTGTTTTAGCATATCAAACTGAATTTGGTGGTGTGTTATCTAAGGAAATGATTGATGCGGCTGTAACAGCTGGTAAAATTTCTTCAAAAGTAGCTACTGATTTAAAAAATTCATTAGAGAGTTTTTCTAAAGGAAAAGATGTTATTACTGGTGACCAAAACATCTGA", "aa": "MQRSPIGLDGFIYGILGRALMPFGLHHIPIVLAYQTEFGGVLSKEMIDAAVTAGKISSKVATDLKNSLESFSKGKDVITGDQNI", "aa_hexdigest": "a473ba75fcc88d38b11e284fafc49bdf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8979.317399999998, "isoelectric_point": 6.709013175964355 }, "id": "EFPKPENHBP_1092", "locus": "GCA000186005_01064" }, { "type": "cds", "sequence": "contig_1", "start": 589356, "stop": 589541, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTAAATACAAGTATTTTTGGTGGTATGGTTATTGGAATTGTCACTGCATATACATTTAATTATTTCTCTAAAATTGAATTACCACCTGCACTTTCATTCTTCTCAGGAATTAGATTAGTGCCAATTATTTTAGTACCTATTTCTTTTGGTGTATCTATTGTATTCTTATTATTATGA", "aa": "MKSLNTSIFGGMVIGIVTAYTFNYFSKIELPPALSFFSGIRLVPIILVPISFGVSIVFLLL", "aa_hexdigest": "3096ce78a6fd002e495bc430cc4f19ad", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6654.0108999999975, "isoelectric_point": 9.522705650329591 }, "id": "EFPKPENHBP_1093", "locus": "GCA000186005_01065" }, { "type": "cds", "sequence": "contig_1", "start": 589584, "stop": 589877, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCTATTGCAATTTTACCTATTGCTGGTTTATTATTAGGAATCGGTGGAGCTATTGGTGCAAATACAACTAATAGTGCTGGTCAAACAACTGCTAATATTTTCAAAGGTGTATCTGAAGTTATTTTTGGAAACTTACCTATTTTATTCTGTATTGCCATAGCGATCACATTTTCAAAAGACAAAGGTGGAGCAGGATTCTCGGCTGCTATTGCTTACCTTGTCTTTACTAGTTCTCAATTAGCATTCATCCAATTTGATGGTGTTGATATTGTTAAAAGTATTTTTTGA", "aa": "MLPIAILPIAGLLLGIGGAIGANTTNSAGQTTANIFKGVSEVIFGNLPILFCIAIAITFSKDKGGAGFSAAIAYLVFTSSQLAFIQFDGVDIVKSIF", "aa_hexdigest": "cae89d5cef978e68e85658c77fc48a9f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9895.530799999991, "isoelectric_point": 5.916791343688964 }, "id": "EFPKPENHBP_1094", "locus": "GCA000186005_01066" }, { "type": "cds", "sequence": "contig_1", "start": 590208, "stop": 590954, "strand": "+", "frame": 3, "gene": "cutC", "product": "PF03932 family protein CutC", "db_xrefs": [ "KEGG:K06201", "RefSeq:WP_013526898.1", "SO:0001217", "UniParc:UPI0001F5ABFD", "UniRef:UniRef100_C4XF03", "UniRef:UniRef50_A0A3P8LIN9", "UniRef:UniRef90_C4XF03" ], "nt": "ATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGGTTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGGTCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAGACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATAGTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAA", "aa": "MKNIVTEVAVDSYQSALNADLAKAKRVELCSDLVLGGLTPSVGLYNLVRKNTKLDIVVMIRPRSGDFCYSASEIEHIIEDIKVFSSLKADALVVGILNGDFSINLEAMKKVIKAAKNTPIVFHRAFDVVNDQFKELENLKRLGVKRILTSGAKPNCDLGLERIKELVKVANGEITIMPGAGVNANNADKIVQETKATEIHFTAKSIVNTSSLDKSKEVNYSSKELNEHLKIESEVAKITEIIDKIKAA", "aa_hexdigest": "111ce63fb4a9eedea60cd8d3f8d47cd4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F5ABFD", "ncbi_nrp_id": "WP_013526898.1", "uniref100_id": "UniRef100_C4XF03", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5ABFD", "RefSeq:WP_013526898.1", "UniRef:UniRef100_C4XF03" ] }, "ips": { "uniref100_id": "UniRef100_C4XF03", "uniref90_id": "UniRef90_C4XF03" }, "psc": { "uniref90_id": "UniRef90_C4XF03", "product": "PF03932 family protein CutC", "uniref50_id": "UniRef50_A0A3P8LIN9", "kegg_orthology_id": "K06201" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LIN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LIN9" ], "product": "PF03932 family protein CutC" }, "genes": [ "cutC" ], "id": "EFPKPENHBP_1095", "locus": "GCA000186005_01067" }, { "type": "cds", "sequence": "contig_1", "start": 591186, "stop": 591305, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGA", "aa": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASM", "aa_hexdigest": "acea58cda84d9cc5bb1a24e6c4432506", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4389.0560000000005, "isoelectric_point": 9.10114688873291 }, "id": "EFPKPENHBP_1096", "locus": "GCA000186005_01068" }, { "type": "cds", "sequence": "contig_1", "start": 591315, "stop": 591533, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAATAAATAGCTACTATGTTACTGAAAGATGA", "aa": "MKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRINSYYVTER", "aa_hexdigest": "610e9e4f4734bb1e96dbed18d9de0d5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8511.816199999997, "isoelectric_point": 10.163263511657714 }, "id": "EFPKPENHBP_1097", "locus": "GCA000186005_01069" }, { "type": "cds", "sequence": "contig_1", "start": 591585, "stop": 592178, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_A5IY48", "UniRef:UniRef90_C4XF04" ], "nt": "ATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAG", "aa": "MEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK", "aa_hexdigest": "87e883a310d404affcc9da91bef69281", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 591285, "stop": 592178, "observations": { "mutation": [ 591303, 591531 ], "stop": [ 591303, 591531 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF04", "query_cov": 1.0, "subject_cov": 0.5969696969696969, "identity": 1.0, "score": 324.0, "evalue": 2.85e-109, "gene_start": 1, "gene_end": 197, "reference_start": 134, "reference_end": 330, "reference_sequence": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASMWNPRMKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRINSYYVTERWLGGTLTNSSTIFKRVKTMEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK" }, "paralog": false, "identity": 0.9932659932659933, "score": 526.0, "evalue": 4.58e-191, "description": "Internal stop codon at 591303, 591531. Nonsense mutation around 591303, 591531.", "psc": { "uniref90_id": "UniRef90_C4XF04", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_A5IY48", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF04", "UniRef:UniRef50_A5IY48", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY48" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "EFPKPENHBP_1098", "locus": "GCA000186005_01070" }, { "type": "cds", "sequence": "contig_1", "start": 592187, "stop": 592276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGA", "aa": "MDKLGLIKELRERTNGGMVDCKKALEESN", "aa_hexdigest": "3f533d98b1c752413aa9c3129e34b77a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3278.778800000001, "isoelectric_point": 6.088445091247558 }, "id": "EFPKPENHBP_1099", "locus": "GCA000186005_01071" }, { "type": "cds", "sequence": "contig_1", "start": 592397, "stop": 593059, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_A5IY49", "UniRef:UniRef90_C4XF05" ], "nt": "ATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAGCTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAA", "aa": "MVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVEAKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK", "aa_hexdigest": "da67cf1959e64fb9ca5ad5025aa9f57e", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "5-prime", "pseudogene": { "start": 592175, "stop": 593059, "observations": { "mutation": [ 592274, 592298 ], "stop": [ 592274, 592298 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF05", "query_cov": 1.0, "subject_cov": 0.7482993197278912, "identity": 1.0, "score": 416.0, "evalue": 9.07e-146, "gene_start": 1, "gene_end": 220, "reference_start": 75, "reference_end": 294, "reference_sequence": "MGVTMDKLGLIKELRERTNGGMVDCKKALEESNWDIEKAITWLKSNGKIKAAKKANRVSAEGLVAIAANATKAVMVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVEAKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK" }, "paralog": false, "identity": 0.9897959183673469, "score": 556.0, "evalue": 3.97e-203, "description": "Internal stop codon at 592274, 592298. Nonsense mutation around 592274, 592298.", "psc": { "uniref90_id": "UniRef90_C4XF05", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_A5IY49", "go_ids": [ "GO:0003746", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF05", "UniRef:UniRef50_A5IY49", "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY49" ], "product": "Elongation factor Ts" } }, "id": "EFPKPENHBP_1100", "locus": "GCA000186005_01072" }, { "type": "cds", "sequence": "contig_1", "start": 593521, "stop": 593820, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06", "UniRef:UniRef90_C4XF06" ], "nt": "ATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAA", "aa": "MENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF", "aa_hexdigest": "76153966046da841818a9a276aaf90d7", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 593290, "stop": 593820, "observations": { "mutation": [ 593461 ], "stop": [ 593461 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF06", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 1.0, "score": 199.0, "evalue": 6.23e-64, "gene_start": 1, "gene_end": 99, "reference_start": 78, "reference_end": 176, "reference_sequence": "MMETKITTSNEQLPILKPILLASDFVEKIKGDKLYSDVMMLIAQDEDYVYFVSCLKEWEKNYELEPRFIKIYEEEGIMENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF" }, "paralog": false, "identity": 0.9886363636363636, "score": 345.0, "evalue": 1e-123, "description": "Internal stop codon at 593461. Nonsense mutation around 593461.", "psc": { "uniref90_id": "UniRef90_C4XF06", "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF06", "UniRef:UniRef50_C4XF06" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11499.206599999983, "isoelectric_point": 9.413754081726076 }, "id": "EFPKPENHBP_1101", "locus": "GCA000186005_01073" }, { "type": "cds", "sequence": "contig_1", "start": 593915, "stop": 594034, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTATTTTAGCTTTAGATAATACTTTGGATAATAAAATTATGAAAGCCAAAAATGCTGATGAAATTTATGAAGTAATGAGTAAATTTAATGTTGATATGCACCAATACGAATAA", "aa": "MISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE", "aa_hexdigest": "969d29bc32388b7cc542439509c441fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4577.236600000001, "isoelectric_point": 4.780352592468262 }, "id": "EFPKPENHBP_1102", "locus": "GCA000186005_01074" }, { "type": "cds", "sequence": "contig_1", "start": 594122, "stop": 594394, "strand": "-", "frame": 3, "gene": null, "product": "PTS EIIA type-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07", "UniRef:UniRef90_C4XF07" ], "nt": "ATGAAATTCAATAATTTTTTAAACAAAAATTCTATTTTTGTACATTCAAATTTTGATAAAAAAGATGATGTTTTGCACTTTTTATCACATCAACTAAATGAACAAAACTTTGTTAGTGATGAAAAGAAAATATATGAAGCTTTTTTATATAGAGAAGAATTAATGAATACTGCAATAGGCGGTGGCATTGCAATTTGTCAAGCATTAAGCTCAACAGTTAAAAAAGACACATTATGTATTTTAACTATGAATAATGATGGGGTTAATGATTGA", "aa": "MKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVND", "aa_hexdigest": "00845ba209b2df49145b0c4e31cd8a4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 593915, "stop": 594436, "observations": { "mutation": [ 594124 ], "stop": [ 594124 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF07", "query_cov": 1.0, "subject_cov": 0.5202312138728323, "identity": 1.0, "score": 181.0, "evalue": 7.9e-57, "gene_start": 1, "gene_end": 90, "reference_start": 15, "reference_end": 104, "reference_sequence": "MRKKRNLIIERTTKMKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVNDWKADDEKNVNLVFALVLSKNNRNLQIDTLQMISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE" }, "paralog": false, "identity": 0.9884393063583815, "score": 339.0, "evalue": 1.75e-121, "description": "Internal stop codon at 594124. Nonsense mutation around 594124.", "psc": { "uniref90_id": "UniRef90_C4XF07", "product": "PTS EIIA type-2 domain-containing protein", "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF07", "UniRef:UniRef50_C4XF07" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07" ], "product": "PTS EIIA type-2 domain-containing protein" } }, "id": "EFPKPENHBP_1103", "locus": "GCA000186005_01075" }, { "type": "cds", "sequence": "contig_1", "start": 594583, "stop": 594828, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAAAACTTAATTATTTGATTGAGGGCGGTAGAGTTAATAATAGCTTTAAAAAATTCGTTTTAAAAACAATGTCTAAATTAAGAATTTATCCTTATGTTAAGTTTATTAAACAACATGTTTCAACAGCTGGAATAGCTCGTGGTGTTAAGGGTGCAATAATTCAAATATTTAAAAAATTAGGAGCTTTCGTTAAAGCAAAAGATCTCAAAACATTAACAGAAAACTTCAAAATTGACTGA", "aa": "MPIKLNYLIEGGRVNNSFKKFVLKTMSKLRIYPYVKFIKQHVSTAGIARGVKGAIIQIFKKLGAFVKAKDLKTLTENFKID", "aa_hexdigest": "4667e27c91505db3ca2c179c5788b992", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9217.032699999992, "isoelectric_point": 10.371818733215331 }, "id": "EFPKPENHBP_1104", "locus": "GCA000186005_01076" }, { "type": "cds", "sequence": "contig_1", "start": 594949, "stop": 595296, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGGTTTTATTGTTGACGGCTTTATTAGTGAACCGCAAGAACTAATTCTTAAAAAAGGTTATGGTTATTTTCCTTTTTTAACAGATATTGATGGTGTAGTTTATAAAGATGGTATTGACTTAGACCAAGAAGATTTATTGCATAAACTTGATCAAGGAAAATCAGTTAAATCATCTATGCCAAATGTTAAAGCTGTTTTAGATACAGTGGACAAAATGACTAAAGAATATGATGAAGTAATTTACTTCCCAATTGGTTCATCATTATCTTCAAGTTGCAACAACATTGCAACAATTTGCAAAGAATATAAAAACTTTCACGTTTATGATAACTGCTGA", "aa": "MKKIGFIVDGFISEPQELILKKGYGYFPFLTDIDGVVYKDGIDLDQEDLLHKLDQGKSVKSSMPNVKAVLDTVDKMTKEYDEVIYFPIGSSLSSSCNNIATICKEYKNFHVYDNC", "aa_hexdigest": "b035d9d7828fa00fd8885e999c2c6109", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.75079999999, "isoelectric_point": 4.889824485778808 }, "id": "EFPKPENHBP_1105", "locus": "GCA000186005_01077" }, { "type": "cds", "sequence": "contig_1", "start": 595489, "stop": 595914, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATTGTGAAGAAGATGAAAACATTATTTTGGAAACAAATGAATATGACTTAATTAATTTAAATACATATGATCGACCAAAAACAACTTTATTAATTAAACCATTACAAAACGGATTTAATTATCGATTAATTAACAATGCTTTTAGTAATTTGAAAGAAGGCATTAACTTAGGTTTATATATCGAAAAGATAAATGACCGTTTAACTAACTATGTTAATATCGGAAATATCAAGGTTTTTGTTATATCGGAAAAGATTTGCAAATACATTGAAAAAGATCAAATTTTATACATAAAACTTTTAAACTTCGTTAAAACAATTAGCACTATTAAAAATCATGTTATTATTTATCAAAACTTACCAGAAGGCTTAGATGAACTTATGGTAAAAACATTAGATGTACAATATGTTTGCAAATAG", "aa": "MENCEEDENIILETNEYDLINLNTYDRPKTTLLIKPLQNGFNYRLINNAFSNLKEGINLGLYIEKINDRLTNYVNIGNIKVFVISEKICKYIEKDQILYIKLLNFVKTISTIKNHVIIYQNLPEGLDELMVKTLDVQYVCK", "aa_hexdigest": "a9dbe22fda2d6b8cf452d580def35fcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16537.991999999987, "isoelectric_point": 5.228698539733886 }, "id": "EFPKPENHBP_1106", "locus": "GCA000186005_01078" }, { "type": "cds", "sequence": "contig_1", "start": 595954, "stop": 596115, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATTATGAAAAGAAAAATGAATTGCAACAATATATAAAAGAAAACATTCCAATTATTTCATATAAAACTAAACGTCAAAGCAAAGTTGCAATTTTAAAAAATAGAGTCATAGGTTACTCTGACCAAGATTCAGAATTCTTTAATAATGTTTCGTGA", "aa": "MLDYEKKNELQQYIKENIPIISYKTKRQSKVAILKNRVIGYSDQDSEFFNNVS", "aa_hexdigest": "2770d6336b0c9917ac49cd5717d50604", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6328.123400000001, "isoelectric_point": 9.003799629211429 }, "id": "EFPKPENHBP_1107", "locus": "GCA000186005_01079" }, { "type": "cds", "sequence": "contig_1", "start": 596167, "stop": 596535, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTTATGCTTTGGGCATCAATGAATTTCTCTACAATAATGTCATTAGTAAATCAGACATTGAAGATATTTTGAGAATATTTGAGCTTAATAATATGTTTGGATATTACTACGTTAAAAACGGATTAATATTATTAATCTTTGAAACCAATTCGTCATTCTGGCTAAACCACTACACTAAAAAAGCACTTAAAAGATTAAAATTCTTACATGAAAGAAAAATTATTAATCCATTTTTCAATAGCTTTGCTTTTTTAAGTGTTAACACTTTCAAAAGTGTTGATGATGTTCAAGAATTAGTTGATAAATCAAAATACTTAATACAACACTTAAATAACAAACGCAAATTTGATAAATATTCTTCTTGA", "aa": "MAYALGINEFLYNNVISKSDIEDILRIFELNNMFGYYYVKNGLILLIFETNSSFWLNHYTKKALKRLKFLHERKIINPFFNSFAFLSVNTFKSVDDVQELVDKSKYLIQHLNNKRKFDKYSS", "aa_hexdigest": "9ce520f26f5d313bc2eb115ff611fbaf", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14595.680999999973, "isoelectric_point": 9.371076011657713 }, "id": "EFPKPENHBP_1108", "locus": "GCA000186005_01080" }, { "type": "cds", "sequence": "contig_1", "start": 596629, "stop": 597105, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA", "UniRef:UniRef90_UPI000FF46DBA" ], "nt": "ATGGATCAATTATTATTGGGAATAGCAATTATAATAGCATGTGTTATTTTAATAGACATCTTTATTATAGTTTTTATTTACTTAAATCATGTTCATAAAGTCAAAACAAATGGTTTAGCAATCTTTAAATTAGACTTTGGACAAAAAAAAATAATTCGCTTGAGTTCTAAAAGCTTAAGTGGATCTTTAAACATGGACTCACGTCTTCAAGGTCTTTCAGCTAATCAATATGTCGACTTTGATGTCTTTCTTTCTTACTTAGATGAAGAGAGCCAAAAAAACTTGACTGAATATCTTTCACAAAACGACAATAATGATAAAAGATTTAATATTTACTGCAAGATGAGTTCTAAAGGCTATCGAACACATCAAAATGCTCAAGGTTTAAAACTTCTAAACATTAAAATGACTAAAGAAAAAATTCTTTTAAAATTCTTCCCTCTTGGAAATAATCGATACTACTTAACAATCCACTGA", "aa": "MDQLLLGIAIIIACVILIDIFIIVFIYLNHVHKVKTNGLAIFKLDFGQKKIIRLSSKSLSGSLNMDSRLQGLSANQYVDFDVFLSYLDEESQKNLTEYLSQNDNNDKRFNIYCKMSSKGYRTHQNAQGLKLLNIKMTKEKILLKFFPLGNNRYYLTIH", "aa_hexdigest": "7982d92f1d45b1c475cf241714921b50", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF46DBA", "query_cov": 1.0, "subject_cov": 0.8540540540540541, "identity": 0.9940000000000001, "score": 297.0, "evalue": 1.9e-101, "valid": true, "uniref50_id": "UniRef50_UPI000FF46DBA" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF46DBA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18272.16730000001, "isoelectric_point": 9.394155693054199 }, "id": "EFPKPENHBP_1109", "locus": "GCA000186005_01081" }, { "type": "cds", "sequence": "contig_1", "start": 597149, "stop": 597652, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "ATGACAGTCCACTCTGCTAAAGGATTAGAATTTGACAATATCTTTTTAGTGGGAATGAGTGATCAAATTTTCCCTAACTATAAATCATTAAAATCAAACTCAGATGATGCTTTAGAAGAAGAAAGAAGACTTGCTTATGTGGCAATTACCAGAGCAAGAAATAGATTATTTATTAGCGATAGTCGTGGATATTTAATTGGTACAGATTTAGAAAAGAAACCATCACGTTTTATTCGTGAAATGGGAATTGAACTTAAAAACTTTATTCTTAAAACAGGTTCTGTTGGCCTTGATTTTGATGAAATAGTTGACGAAAAACAAATAAAAGCTTTGAACAAAAAAATTATAGTAGGTGACATTATTAGCCACACCTTCTTTGGCGAAGGCCAAGTTGTGGCTGTTGAAAACGATACAATTAGCGTTAGATTCACAGGACAAAAAACAATCAAAGTTTTAGCTAAAAATCACGCTTCAATTCGACTTTTAAAAAATACAGATGATTAA", "aa": "MTVHSAKGLEFDNIFLVGMSDQIFPNYKSLKSNSDDALEEERRLAYVAITRARNRLFISDSRGYLIGTDLEKKPSRFIREMGIELKNFILKTGSVGLDFDEIVDEKQIKALNKKIIVGDIISHTFFGEGQVVAVENDTISVRFTGQKTIKVLAKNHASIRLLKNTDD", "aa_hexdigest": "61feab745687eac4e2a0d90e0d4f1d63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 61, "aa_cov": 0.3652694610778443, "hmm_cov": 0.9672131147540983, "evalue": 1.891220968016977e-26, "score": 89.60643005371094, "start": 1, "stop": 61 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 23, "aa_cov": 0.1377245508982036, "hmm_cov": 1.0, "evalue": 6.955912266099755e-11, "score": 38.21699523925781, "start": 36, "stop": 58 } ], "seq_stats": { "molecular_weight": 18852.393399999997, "isoelectric_point": 7.916862678527832 }, "id": "EFPKPENHBP_1110", "locus": "GCA000186005_01082" }, { "type": "cds", "sequence": "contig_1", "start": 597755, "stop": 598309, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "GTGGTCCAAAAAATTAATGAATTAAAGAAACAAAAAATTCAGCTTAAAAATATTGCTATTTTTTACCGTTCAAATTATTATTCGCGTCCTTTCGAAGAAGAATTAATTAATGAAAATATTAACCACAAGATTTTTAATGGTCAAAAATTCTTCCAAAGAACTGAAGTTAAGAATGCTCTAGCATTTTTAAGAGTTATCTATGATGGCTCTGATGTTTCATTAAATAGAATAATTAATGTTCCAAACCGTAAAATCGGAGAAGCAACTCTTAAAAAATTACAAGAAGCAGCAAATAATAAAAAATTAACTCTCTATTCATATTTAATTAAATATTACAAGGAATTGCCAGTACCTTTAAATGTTAAAAACAACATTGTTAAGTTTCTTAACAAAATCAACCTTTATCGTCAAGCTTTAAAAAGCAACTCAATTAGTGTTGTTTTAGATAAATTCTTAAAGGAAATTGGCTACTATGAACATATAGAAGAAGAAACCTCACTTCGTGGCACTGCTAAAGATAATGTTGCTGAATTAATTCGCTCAATAGTAACTTGA", "aa": "MVQKINELKKQKIQLKNIAIFYRSNYYSRPFEEELINENINHKIFNGQKFFQRTEVKNALAFLRVIYDGSDVSLNRIINVPNRKIGEATLKKLQEAANNKKLTLYSYLIKYYKELPVPLNVKNNIVKFLNKINLYRQALKSNSISVVLDKFLKEIGYYEHIEEETSLRGTAKDNVAELIRSIVT", "aa_hexdigest": "5e395766e0c7a5ec03084fa9c6a9d23f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 109, "aa_cov": 0.5869565217391305, "hmm_cov": 1.0, "evalue": 4.0307983522269165e-24, "score": 81.94786834716797, "start": 2, "stop": 109 } ], "seq_stats": { "molecular_weight": 21590.796600000016, "isoelectric_point": 9.696447944641115 }, "id": "EFPKPENHBP_1111", "locus": "GCA000186005_01083" }, { "type": "cds", "sequence": "contig_1", "start": 598310, "stop": 598519, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "TTGATCCTTGATTTTGATAAGGATTTCAAGAATACTAAAACGATTATTTTAAATACAAATTACCGCTCAACTAAAACAATTTTAGATGCAGCTAACAAATTAATCAAACACAACAAAAAACGTTTTAGTAAAGATTTAATAACAAATCGTGAACAAGGAACACCAATAGAATTTATTCATGCTTTTAGTGTCGAAGCTGAAGCTAGATGA", "aa": "MILDFDKDFKNTKTIILNTNYRSTKTILDAANKLIKHNKKRFSKDLITNREQGTPIEFIHAFSVEAEAR", "aa_hexdigest": "eed357a029a74435c1ec55ba62ef2466", "start_type": "TTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 55, "aa_cov": 0.7971014492753623, "hmm_cov": 1.0, "evalue": 2.13942748728647e-11, "score": 40.09764099121094, "start": 15, "stop": 69 } ], "seq_stats": { "molecular_weight": 8054.158399999998, "isoelectric_point": 9.62907848358154 }, "id": "EFPKPENHBP_1112", "locus": "GCA000186005_01084" }, { "type": "cds", "sequence": "contig_1", "start": 598874, "stop": 599320, "strand": "-", "frame": 3, "gene": null, "product": "UvrD-helicase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "nt": "ATGAGCAAACGAGATGAAATTTTGAATTCATTAAATGCTGAACAAAAAGAAGCTTTAATGTATTTTGATGGTCCTTTAAGAATCATCGCTGGAGCTGGAAGTGGTAAAACTAGAGTTTTAACTAGGAAGGTGGCTTATTTAATTAATGAACTTGGAATTAGCCCACGTTCAATTTTAGCAGTGACTTTCACTAATAAAGCTGCGAACGAAATGAGCGAACGTGTTAAACAATACGTCGGCGAAGAAGCTAATCAAATCGATATTTGTACTTTCCACTCTTTAAGCTCAAAGATTTTAAGGAAGGAAGCTATTCATGTTAATTTAAGTAATGACTTCCAAATTATTGATGAAACAGATAAGAAGACAATTATTGCTCAACTTTATAAAGCTGAAGGCATTTCAAATGAAGATGTTTCATACAAAAATGTTATGAAGATTTTCTCATGA", "aa": "MSKRDEILNSLNAEQKEALMYFDGPLRIIAGAGSGKTRVLTRKVAYLINELGISPRSILAVTFTNKAANEMSERVKQYVGEEANQIDICTFHSLSSKILRKEAIHVNLSNDFQIIDETDKKTIIAQLYKAEGISNEDVSYKNVMKIFS", "aa_hexdigest": "3ac20ae09ec7741bfea9f24769d48083", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002961EF37", "query_cov": 0.8513513513513513, "subject_cov": 0.9197080291970803, "identity": 0.508, "score": 139.0, "evalue": 6.76e-40, "valid": false, "product": "UvrD-helicase domain-containing protein", "uniref50_id": "UniRef50_UPI002204F9A8" }, "pscc": { "uniref50_id": "UniRef50_UPI002204F9A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "product": "UvrD-helicase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1113", "locus": "GCA000186005_01085" }, { "type": "cds", "sequence": "contig_1", "start": 599333, "stop": 599491, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATGGCTTTAAGAACCAGACTTGGACTTTTTGATCGTTCAACAAACACCAGAGTAACATACTTAAAACATTACTCACAATCAAGCTATTTAGATGAAATCAAACTGTTACCATTCAAAAGTAATAATGAAGCTGTAATTTACATTAAAAAATAG", "aa": "MEMALRTRLGLFDRSTNTRVTYLKHYSQSSYLDEIKLLPFKSNNEAVIYIKK", "aa_hexdigest": "24259b18c6c6dda4e02fcec5cf01e20e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.107499999999, "isoelectric_point": 9.695158576965333 }, "id": "EFPKPENHBP_1114", "locus": "GCA000186005_01086" }, { "type": "cds", "sequence": "contig_1", "start": 599660, "stop": 599881, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCATAAAGGTGTTGTCAAGGCAATTAACTTTTATTCTTTTATTATATTTTCAACAGTTATTTTACTTGTGCTATTTTTACTAATTCCATTGTTTTATAGTAGTAGAACAATTGGAATGCTGATTTTTAGAGTACAAATAATACCTGCTAAAAAGATGACTCCTTTAGCTTTAAAATTATTACCTTTAAGAAGAAATGCATTTGCTTTAATTTTCTGA", "aa": "MVHKGVVKAINFYSFIIFSTVILLVLFLLIPLFYSSRTIGMLIFRVQIIPAKKMTPLALKLLPLRRNAFALIF", "aa_hexdigest": "364b28c26d48461e1e7a845902056279", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8381.402699999991, "isoelectric_point": 11.614382362365724 }, "id": "EFPKPENHBP_1115", "locus": "GCA000186005_01087" }, { "type": "cds", "sequence": "contig_1", "start": 600619, "stop": 600999, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCAATTATTTCACCAGGAATAAATAGCGCTAAAAGTTTAAAAAATATTTTAGAAGGTATGAAAGATCAAAATAATCAAGATTTTGAATTAATTTTAGCTTTAAGAAACCCTTCAGCTAAAATGTATTCTATTATTGAAAGATATTTAGCATTCTTTGGCACACGTTTAAAATTTATTACTAATAATCGTCAGAGAACTATGCAAAGTGATATAGTTTGTGCTTTTCACTTAGTTAAAGGAAACTATGCCACAATTATTAATAGTGACAACTCAATGCGTTCTTACTTTGCACAAGAATTAACAAACTATATTGAAAAATTTAATCCAGATGTTTTAGAATATCGTCCAAGATTGGTTGGCACAGTTAAATGA", "aa": "MKLSIISPGINSAKSLKNILEGMKDQNNQDFELILALRNPSAKMYSIIERYLAFFGTRLKFITNNRQRTMQSDIVCAFHLVKGNYATIINSDNSMRSYFAQELTNYIEKFNPDVLEYRPRLVGTVK", "aa_hexdigest": "93ef7331a216a15f14767417a79719b9", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 111, "aa_cov": 0.8809523809523809, "hmm_cov": 0.990990990990991, "evalue": 5.2887347341892454e-11, "score": 39.0565185546875, "start": 4, "stop": 114 } ], "seq_stats": { "molecular_weight": 14546.66419999998, "isoelectric_point": 9.586658287048337 }, "id": "EFPKPENHBP_1116", "locus": "GCA000186005_01088" }, { "type": "cds", "sequence": "contig_1", "start": 601009, "stop": 601143, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGCGTCGTTTTGGCGATAGTGGTTCAAGCGGTTTTGGAGTTGGTTTTGAAAGATTAGTAATGTATGTCACAGGTGTTGACAATATTAGAGATGTTATTCCATATCCAAGAACATCTGGAAATATTAAAATGTAA", "aa": "MRRFGDSGSSGFGVGFERLVMYVTGVDNIRDVIPYPRTSGNIKM", "aa_hexdigest": "3d7ca7e8900fcfe6bdb806abfc6963ef", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 0.9318181818181818, "subject_cov": 0.8541666666666666, "identity": 0.659, "score": 60.8, "evalue": 1.55e-11, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 36, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 7.49289804262544e-11, "score": 37.924766540527344, "start": 3, "stop": 38 } ], "seq_stats": { "molecular_weight": 4883.5452000000005, "isoelectric_point": 9.684585762023925 }, "id": "EFPKPENHBP_1117", "locus": "GCA000186005_01089" }, { "type": "cds", "sequence": "contig_1", "start": 601153, "stop": 601668, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGAGCCTGAAGTTGCTTTCTATGAATTAAAGGATGCAATCAAATTAGCTGATGACATGTTAAAATCTGTTATCAAAAATACAATTAAAAAACATCCTGCTGAATTTAAATTTTTAAATGAAACAGTTGACAAAGGTCTTTTAGAAAGATTAAATCACTTTTTAAAAACAAAACTTAGTGTTATTGACTATCGCGACGCAATTAAAGAATTGGCTAAAGTTAAAGACCAATTTGAAAATCAAGATATTCAATTCGGACTTGATTTAGCAACTGAACATGAAAGATATATTTCAGAAAAAATCTTTAATGGACCAGTAGCTGTAATTAATTTTCCAAAAAGTTTTAAAGCATTCTATATGCAACAAAATGATGACAATGAAACTGTAGCAGCTTTTGACTTATTAGTGCCAGGAATTGGCGAATTAATAGGTGGAAGCCAAAGAGAAGTTAGATATGACAAATTACTTCAAAGAGCTTTAGAAGTAGGAATCAGTCAAGAAGAATTGCAATGA", "aa": "MIEPEVAFYELKDAIKLADDMLKSVIKNTIKKHPAEFKFLNETVDKGLLERLNHFLKTKLSVIDYRDAIKELAKVKDQFENQDIQFGLDLATEHERYISEKIFNGPVAVINFPKSFKAFYMQQNDDNETVAAFDLLVPGIGELIGGSQREVRYDKLLQRALEVGISQEELQ", "aa_hexdigest": "9137cbf77936c547f6e0cee74be3d316", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 178, "aa_cov": 0.9883040935672515, "hmm_cov": 0.8089887640449438, "evalue": 3.2294742715076445e-21, "score": 71.9686508178711, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19665.288799999988, "isoelectric_point": 5.082110786437987 }, "id": "EFPKPENHBP_1118", "locus": "GCA000186005_01090" }, { "type": "cds", "sequence": "contig_1", "start": 601669, "stop": 601965, "strand": "-", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "nt": "ATGTTAATAAGATCAACGTTAGCTAAAGAAGTTAATAAATATTTTGATGAACACGGTTACTTATACTTCAATGCTCCTATTATTACCAGCAATGATGGTGAAGGAGCTGGAGAAACTTTTGTAGTTAATGATGGAAATTCAAAAGATCCTTTTTTTGGGACTAGAAAAGCTACTTTAGGAGTTACAGGACAATTGCATGCTGAAAGTTATGCAATTGGATTTAAAAAAGTTTATACTTTTGCACCTACTTTTAGAGCAGAACACTCAAATACCAAAAAACATGCAGCAGAATTTTGA", "aa": "MLIRSTLAKEVNKYFDEHGYLYFNAPIITSNDGEGAGETFVVNDGNSKDPFFGTRKATLGVTGQLHAESYAIGFKKVYTFAPTFRAEHSNTKKHAAEF", "aa_hexdigest": "c8911058b0c3505beaa206642a212cb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387B779D", "query_cov": 0.8775510204081632, "subject_cov": 0.8514851485148515, "identity": 0.674, "score": 116.0, "evalue": 4.51e-32, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00387B779D" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B779D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "EFPKPENHBP_1119", "locus": "GCA000186005_01091" }, { "type": "cds", "sequence": "contig_1", "start": 602657, "stop": 602986, "strand": "+", "frame": 2, "gene": null, "product": "PQ-loop repeat-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35", "UniRef:UniRef90_UPI000FF44E35" ], "nt": "ATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGA", "aa": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLL", "aa_hexdigest": "b37ea1e873c200715c04adc101b217a4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 602657, "stop": 603196, "observations": { "mutation": [ 602984, 603071 ], "stop": [ 602984, 603071 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44E35", "query_cov": 1.0, "subject_cov": 0.5989010989010989, "identity": 1.0, "score": 205.0, "evalue": 5.96e-66, "gene_start": 1, "gene_end": 109, "reference_start": 1, "reference_end": 109, "reference_sequence": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLLWILALSGLVFFIVVYANKNARTNLGPDNGWMIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGKLY" }, "paralog": false, "identity": 0.9888268156424581, "score": 342.0, "evalue": 2.09e-122, "description": "Internal stop codon at 602984, 603071. Nonsense mutation around 602984, 603071.", "psc": { "uniref90_id": "UniRef90_UPI000FF44E35", "product": "PQ-loop repeat-containing protein", "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44E35", "UniRef:UniRef50_UPI000FF44E35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35" ], "product": "PQ-loop repeat-containing protein" } }, "id": "EFPKPENHBP_1120", "locus": "GCA000186005_01092" }, { "type": "cds", "sequence": "contig_1", "start": 603074, "stop": 603259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGA", "aa": "MIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGIVLNALLCIYLGLVVPFNTAT", "aa_hexdigest": "ebb8bc4c1a04248b81aee1cce0420332", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 49, "aa_cov": 0.8032786885245902, "hmm_cov": 1.0, "evalue": 1.1023054200503825e-08, "score": 31.071535110473633, "start": 3, "stop": 51 } ], "seq_stats": { "molecular_weight": 6462.937399999999, "isoelectric_point": 8.45897731781006 }, "id": "EFPKPENHBP_1121", "locus": "GCA000186005_01093" }, { "type": "cds", "sequence": "contig_1", "start": 603495, "stop": 603701, "strand": "+", "frame": 3, "gene": null, "product": "Aminopeptidase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7", "UniRef:UniRef90_A0A3P8MEW7" ], "nt": "ATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGA", "aa": "MQINKKEINNLYKKYLKNSNNKLIESAVTKNGIFNASYNNDMQTKHNFEFNTEVAKAGMTNQKSSGRC", "aa_hexdigest": "a730b0eb2dd06b5c996edae0430fcb1b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 603495, "stop": 603804, "observations": { "insertion": [ 603750 ], "mutation": [ 603699 ], "stop": [ 603699 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEW7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.8240000000000001, "score": 114.0, "evalue": 1.6e-31, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MKIDKKEINKFYKKYAKNSTNKLIESAMTKNGIFNASYNNDMQVKHNFEFNTQVTKTGITNQKSSGRCWIFSGLNMLKVKASKALMLKILNSAKTTFYFETN" }, "paralog": false, "identity": 0.7941176470588235, "score": 161.0, "evalue": 6.96e-53, "description": "Internal stop codon at 603699. Frameshift due to insertion around 603750. Nonsense mutation around 603699.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEW7", "product": "Aminopeptidase E", "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEW7", "UniRef:UniRef50_A0A3P8MEW7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7" ], "product": "Aminopeptidase E" } }, "id": "EFPKPENHBP_1122", "locus": "GCA000186005_01094" }, { "type": "cds", "sequence": "contig_1", "start": 603966, "stop": 604436, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACATTTAACTCAAGCAATACTCACGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGCGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAATCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAAGATAAAAAATTTATCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAACATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTGCTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAGTCACTTAATGTTACAATGGATGTAATTAAAAAAGCTGTTATTGCTTCATTAAAAGATAATGAGCCAGTTTGA", "aa": "MPETFNSSNTHGLNTALNCQLISATKEIREAIKNKASQEVLMKIKHEALERIFEINAKSLGLPPQKFSFEYRDKDKKFIRIENVTPLEFLEKHVGTDFLNKVNLLDDPRKENPKNRLLVAKYYKSVIEEKGLESLNVTMDVIKKAVIASLKDNEPV", "aa_hexdigest": "ce3d5d4a20a2844e5ec8bbf4be5fb125", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17884.47619999999, "isoelectric_point": 8.87009220123291 }, "id": "EFPKPENHBP_1123", "locus": "GCA000186005_01095" }, { "type": "cds", "sequence": "contig_1", "start": 604512, "stop": 604634, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCAAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGA", "aa": "MLNIKNTLTKEDRVNYRLSVQTHAMTIVGVNLDKDNNPIN", "aa_hexdigest": "2b0d6b4aa31f0a9e3ef043cbe2504b00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4584.1962, "isoelectric_point": 8.210580635070801 }, "id": "EFPKPENHBP_1124", "locus": "GCA000186005_01096" }, { "type": "cds", "sequence": "contig_1", "start": 604933, "stop": 605034, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGA", "aa": "MTAKVIKTIAQVLVVLSLLFICSLAIFNMQAGH", "aa_hexdigest": "5ee581c4bc7dae510991c297905e9406", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3574.4103999999998, "isoelectric_point": 9.305705070495605 }, "id": "EFPKPENHBP_1125", "locus": "GCA000186005_01097" }, { "type": "cds", "sequence": "contig_1", "start": 605323, "stop": 605466, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGA", "aa": "MPKRAKLITANIKPVTKPIKVSFQITLPYSPGSISFKAKARLTEILA", "aa_hexdigest": "e981b0f201ed9c55f48b7cd127fac8e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5168.235, "isoelectric_point": 10.79015407562256 }, "id": "EFPKPENHBP_1126", "locus": "GCA000186005_01098" }, { "type": "cds", "sequence": "contig_1", "start": 605661, "stop": 606254, "strand": "-", "frame": 2, "gene": "mgtE", "product": "magnesium transporter MgtE N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "nt": "ATGAAACTACTACAAGAATTACAAAGTGATGAACTAGCTGATGTTTTAGAAGAATTACCTTCGAATGTCACAAGCAAAATCTTAGCTTATACACCTCAAGAAAAAAGAGCTGAATTAAATAAACTTTTAAGCTATGAAGATGATGAAGTTGGAAGCATAATGTCAATAGACATTTCTTCAATTGGTAATTCATATACTTGCGAACAAGCTTTACATAAAATTAGACGTGATTATCATAAGAAAAATGCTGAATTAGTGCATTACTATTACGTAGTTAATGAAACAAATAAACTAATTGGTGCTTTAACACTAGAAGAAATAGTTTTTGCTGATCCTAATGCTAAAATTGATGATATTTATAATCCTGTAACTTATGTTTCAGCTCATGATAAAACCGAAGTAGCAGCCCAAGTTTTTAGTGAACACGATATGTCAGTATTACCTGTAGTTAACCATGAAAAAAGATTAGTTGGAATGATTACAAGTGACGACGTTATTGATGTTATTAAAGAAGAAGCTACAGAAGATTTATACAAAATGGCAGGTATTAGTAGCCATGAAGTTAATGAAAGTGAATACTTAAAAACTCCATGA", "aa": "MKLLQELQSDELADVLEELPSNVTSKILAYTPQEKRAELNKLLSYEDDEVGSIMSIDISSIGNSYTCEQALHKIRRDYHKKNAELVHYYYVVNETNKLIGALTLEEIVFADPNAKIDDIYNPVTYVSAHDKTEVAAQVFSEHDMSVLPVVNHEKRLVGMITSDDVIDVIKEEATEDLYKMAGISSHEVNESEYLKTP", "aa_hexdigest": "b69a6432d78c5459c90f2ae14c5c1d39", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C659EE", "query_cov": 0.9137055837563451, "subject_cov": 0.8411214953271028, "identity": 0.522, "score": 177.0, "evalue": 6.02e-53, "valid": false, "product": "magnesium transporter MgtE N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0024C659EE" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C659EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "product": "magnesium transporter MgtE N-terminal domain-containing protein" }, "genes": [ "mgtE" ], "id": "EFPKPENHBP_1127", "locus": "GCA000186005_01099" }, { "type": "cds", "sequence": "contig_1", "start": 606258, "stop": 606509, "strand": "-", "frame": 2, "gene": null, "product": "Magnesium transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "nt": "ATGAGCGAAAGTCAAGAGATTAGTTTGCAAAACGAACTACATGAAATTATTAAAACTAAAAATGTAACTAAAGCCAGAGAATTTATTGATAATAATCCAATGGCTGATGTTGCAAACTTATTAAATGAATTACAGTTAGAAGAACAGCTAACTTTTTTGCCTTTACTTAAAACTAGTGAAGCTGCTGAATTATTTAGCTACCTTGATGAAGAAGTCCAAGCTAATTTAGCTCAAAGTTTTACAGAAGATTGA", "aa": "MSESQEISLQNELHEIIKTKNVTKAREFIDNNPMADVANLLNELQLEEQLTFLPLLKTSEAAELFSYLDEEVQANLAQSFTED", "aa_hexdigest": "e79bd320d1434f37e735e5c9a4e1b022", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A3P8KNN3", "query_cov": 1.0, "subject_cov": 0.8645833333333334, "identity": 0.892, "score": 136.0, "evalue": 3.59e-40, "valid": false, "product": "Magnesium transporter", "uniref50_id": "UniRef50_A0A3P8KNN3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "product": "Magnesium transporter" }, "genes": [], "id": "EFPKPENHBP_1128", "locus": "GCA000186005_01100" }, { "type": "cds", "sequence": "contig_1", "start": 606511, "stop": 607092, "strand": "-", "frame": 1, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "RefSeq:WP_013354684.1", "SO:0001217", "UniParc:UPI0001A8C272", "UniRef:UniRef100_C4XF17", "UniRef:UniRef50_L0RXD2", "UniRef:UniRef90_C4XF17" ], "nt": "ATGTGTTTAGAATCTAAAATCGGTTTTTATGTAATTGTTGCATTATTAGTTACTTTAATTTTAATTTCAATAATGCCACTTATAGTTAATGCAATTAAAAACAAAATGGCTAGAAGTCATTTCCAAAAATTAGGTCAAAGTAGTCAAATTAGATTAATTAACCAACTACGTGAAGCTGTTGAATATTTATCAAAAAATAAAATTGGAGCTTTAATTACAATTGAAAACAATGATAATATTGACAATTTGAGAACTGATGGGGTAATTTTAAATGCTAATATCAGTAGTAGCTTACTTATTTCAATATTCAATAAATATAGTCCATTGCATGATGGAGCTGTAATTATAAGAGATAATAAAATTTATTATGCTTCTACTTTTTATAAAATCACCAGAAAATCAATTGATAATCAATATGGTGCTCGTCATAGAGCTGCTATGGGAATTAGTGAACTTTGTGATGCTACCACAATAATTGTTAGTGAAGAAACAGGGGATGTTAAATTTGTTAAAAATGGTACTTTCTACAACATTCGTCTTGAACAATTCCAAGAACAACTAGTTAAATACTTAAAGGATTAA", "aa": "MCLESKIGFYVIVALLVTLILISIMPLIVNAIKNKMARSHFQKLGQSSQIRLINQLREAVEYLSKNKIGALITIENNDNIDNLRTDGVILNANISSSLLISIFNKYSPLHDGAVIIRDNKIYYASTFYKITRKSIDNQYGARHRAAMGISELCDATTIIVSEETGDVKFVKNGTFYNIRLEQFQEQLVKYLKD", "aa_hexdigest": "fd947db9bc030b75f4722be4eed0c1e6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C272", "ncbi_nrp_id": "WP_013354684.1", "uniref100_id": "UniRef100_C4XF17", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C272", "RefSeq:WP_013354684.1", "UniRef:UniRef100_C4XF17" ] }, "ips": { "uniref100_id": "UniRef100_C4XF17", "uniref90_id": "UniRef90_C4XF17" }, "psc": { "uniref90_id": "UniRef90_C4XF17", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_L0RXD2", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_L0RXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXD2" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1129", "locus": "GCA000186005_01101" }, { "type": "cds", "sequence": "contig_1", "start": 607102, "stop": 607404, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526910.1", "SO:0001217", "UniParc:UPI0001F5AC06", "UniRef:UniRef100_C4XF18", "UniRef:UniRef50_C4XF18", "UniRef:UniRef90_C4XF18" ], "nt": "ATGGCAATTATTGATAGAGATCTAGCAACATGTGCTGAAAGAATTCAAAAAAGAAGAGAATTACTATTAAAACTTTATAATGAAAATCAAGCTGAATTAGCTAATCCAGCTTTGATTGAAGATGAAAAACCTATTACTGGTGAATGCCAAGAATTTATGTGTTTAGTAAATAAACATGAGAAAGAAGATCCTAGAGTTAGAAAGCAAAAAATTATTCTTAAAGTTTTAGGAGCTAGCTTATCAGTTTTAATTATTTTACTTTGCACACTACTAGCTGTTTTAATTTCAAGAGTTTATGCTTAG", "aa": "MAIIDRDLATCAERIQKRRELLLKLYNENQAELANPALIEDEKPITGECQEFMCLVNKHEKEDPRVRKQKIILKVLGASLSVLIILLCTLLAVLISRVYA", "aa_hexdigest": "6ab1b92ccf6a12287af4e5fdad6c5583", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F5AC06", "ncbi_nrp_id": "WP_013526910.1", "uniref100_id": "UniRef100_C4XF18", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AC06", "RefSeq:WP_013526910.1", "UniRef:UniRef100_C4XF18" ] }, "ips": { "uniref100_id": "UniRef100_C4XF18", "uniref90_id": "UniRef90_C4XF18" }, "psc": { "uniref90_id": "UniRef90_C4XF18", "uniref50_id": "UniRef50_C4XF18" }, "pscc": { "uniref50_id": "UniRef50_C4XF18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF18" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11402.467599999987, "isoelectric_point": 7.644867134094238 }, "id": "EFPKPENHBP_1130", "locus": "GCA000186005_01102" }, { "type": "cds", "sequence": "contig_1", "start": 607679, "stop": 608041, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FDEF42B", "UniRef:UniRef90_UPI001FDEF42B" ], "nt": "TTGTATTTAAATGAAAGAAAAAAAGAAAAATTAAAAATCGATCCTTTTTATGAAAAAAATCATTTTTATGCAAATTCATATTTAATTGAAAAATTATCGAATTTTAAAAATAATTTTATTTACACAATTTCTATCGTTATAAAAGAAAATTCAGAAGAAAATCAATTATATATAAAGAATATCAAAAAACAGATAGACGAAATTAACAAAAAATTATTTGATAGCGAAAAAACATATTTTAAATTCTTTTATCTAAAAACAATTTTTGTCAAAGCTAAAAAACATTTTAAATTATTTACTAAGAAAATAACAAATAAAAGAAATTTGAAATTAAAAAAATTAAATATTGTTAATGAAAAATAG", "aa": "MYLNERKKEKLKIDPFYEKNHFYANSYLIEKLSNFKNNFIYTISIVIKENSEENQLYIKNIKKQIDEINKKLFDSEKTYFKFFYLKTIFVKAKKHFKLFTKKITNKRNLKLKKLNIVNEK", "aa_hexdigest": "9ea024d4ffb96ecbbc3faf137b086911", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 607679, "stop": 608341, "observations": { "mutation": [ 608098, 608113, 608197, 608263 ], "stop": [ 608098, 608113, 608197, 608263 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FDEF42B", "query_cov": 1.0, "subject_cov": 0.4332129963898917, "identity": 0.992, "score": 222.0, "evalue": 5.58e-71, "gene_start": 1, "gene_end": 120, "reference_start": 158, "reference_end": 277, "reference_sequence": "MDISIVKKILVENKNNLFIIFQWKNEHFNIKLQNKFKIIDFIRNSCEDIEFFSFFDAYCEKNDESNIDLHEILGAKYNKKIWFWTKINNIEKTKLKLTFYYLDSFWNFALFLLPIILALFLNNEIPVLKTFNKWVILFWITFVYLTVSFVRIFFKKDLYLNERKKEKLKIDPFYEKNHFYANSYLIEKLSNFKNNFIYTISIVIKENSEENQLYIKNIKKQIDEINKKLFDSEKTYFKFFYLKTIFVKAKKHFKLFTKKITNKRNLKLKKLNIVNEK" }, "paralog": false, "identity": 0.9818181818181818, "score": 427.0, "evalue": 3.14e-154, "description": "Internal stop codon at 608098, 608113, 608197, 608263. Nonsense mutation around 608098, 608113, 608197, 608263.", "psc": { "uniref90_id": "UniRef90_UPI001FDEF42B", "uniref50_id": "UniRef50_UPI001FDEF42B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FDEF42B", "UniRef:UniRef50_UPI001FDEF42B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FDEF42B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FDEF42B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14804.260199999975, "isoelectric_point": 9.874380683898927 }, "id": "EFPKPENHBP_1131", "locus": "GCA000186005_01103" }, { "type": "cds", "sequence": "contig_1", "start": 608261, "stop": 608512, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATTAGTATAGTAAAAAAAATTTTGGTAGAAAATAAAAACAATTTATTTATTATTTTTCAATGGAAAAATGAACATTTTAATATTAAATTACAAAACAAATTTAAAATTATAGATTTTATTAGGAATAGTTGTGAAGATATAGAATTTTTTAGTTTTTTTGATGCTTATTGTGAAAAAAATGATGAATCAAATATTGATTTACATGAGATATTAGGCGCGAAATATAATAAAAAAATTTGGTTTTGA", "aa": "MDISIVKKILVENKNNLFIIFQWKNEHFNIKLQNKFKIIDFIRNSCEDIEFFSFFDAYCEKNDESNIDLHEILGAKYNKKIWF", "aa_hexdigest": "c46cf596eefbbe7fecec248100533b47", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10140.582799999991, "isoelectric_point": 6.076167869567871 }, "id": "EFPKPENHBP_1132", "locus": "GCA000186005_01104" }, { "type": "cds", "sequence": "contig_1", "start": 609370, "stop": 609534, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAGAGGAATTTTTCAAAGGCGTAAGTAGACCAGTAAATGAAAAATTACAAAAAATTTTTATGCAATTAGATCTAGTTGAACAAACTGGATATGGAGTGCCTTTAATTGTCAAAAAATATGACGAAGAAGCATTTGAAATTGATGAAAATACAATTTGA", "aa": "MTKEEFFKGVSRPVNEKLQKIFMQLDLVEQTGYGVPLIVKKYDEEAFEIDENTI", "aa_hexdigest": "42fc4fc0a2a96e81ee9b060082f65a0a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.2024, "isoelectric_point": 4.609210395812987 }, "id": "EFPKPENHBP_1133", "locus": "GCA000186005_01105" }, { "type": "cds", "sequence": "contig_1", "start": 609640, "stop": 610329, "strand": "-", "frame": 1, "gene": null, "product": "Putative ATP-dependent DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7", "UniRef:UniRef90_Q6GYZ7" ], "nt": "ATGCTCAATAAAAGAGGAACGGGAACAATTTATTTTAGTCTAAAAGATGATCATACAATTGTTGACTTAGATATTTGTAATAAATCCATGAGAGATATTTCTCAAGCTATTTCAAACAATGTTAAGCCAGAAATAATGCCTAGAATTTCGATTGAATTTATTGATAACCAAAATGTAATTAAAGTTGAAGCTGAAGGAGAACAAAAACCTTATTCAGCTTATGATAAATATTATATTCGTATTGGAGAAGAAGATAAAAAACTTAATAGAGATAAATTAGTAGATTTTGTGCAAAAATCAGTTTTTAAAATAAATATTTGTGAAATTGAAAACAAAGATCAAGATTTGACATTTAATAGTTTTTTGCTATATGCTAATATTAAAAATTTAACAGTAAATAAAGATACTTTCGAAAAAAATTTTGATTTAAAGACTAAAAATAATAAATACAATTTAATGTCTTATTTATTATCAAATAATAATGATATTTCAATTAAAGTTAATACTTTTAAAGATAATGATAAAAGTGTTTTGTTGAAAAGAAACGAATATGGAAATAAATGTTTATTTTATGCAATTGAGAGCATTTTAAATTATGTAGGTTCTATTAATGATACTTATGTAGATTTGTCAACTGCAACCAGGAGTAAACAAAAATTATTCGATTTTGAGTGTTTTAGAGAAGCATGA", "aa": "MLNKRGTGTIYFSLKDDHTIVDLDICNKSMRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGEEDKKLNRDKLVDFVQKSVFKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKNFDLKTKNNKYNLMSYLLSNNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESILNYVGSINDTYVDLSTATRSKQKLFDFECFREA", "aa_hexdigest": "4cbfdae80d914b527267de72a2777514", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 609337, "stop": 610417, "observations": { "insertion": [ 610357 ], "mutation": [ 610402 ], "stop": [ 609372, 609615, 609642, 610402 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GYZ7", "query_cov": 1.0, "subject_cov": 0.5264367816091954, "identity": 0.93, "score": 422.0, "evalue": 9e-146, "gene_start": 1, "gene_end": 229, "reference_start": 30, "reference_end": 258, "reference_sequence": "MNKMPWESEEVEYKKSTSELKEAIISLTAMLNKKGTGTVYFGVKDDRTVVGLEIGNKTMRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGEEDKKLNRDKLVDFVQKSVSKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKNFDLKTKNNKYNLMSYLLADNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESVLNYVGSINDTYVDLSTATRSEQKLFNFECFREAWLNACIHNRWDEKYPPQVNIFKDYIQIESNGGIPRNMTKEKFFKGVSRPVNEKLQKIFMQLDLVEQTGHGVPLIVKKYGEEAFEIDENTIWVKIPFNRKGFKRINEKENKNNLNDNQIKVIKTIEKESNITINQLSSTLSLSEGYIKKIINQLKNKNLIERNGLRKNGNWTIISYNL" }, "paralog": false, "identity": 0.9220055710306406, "score": 654.0, "evalue": 1.57e-239, "description": "Internal stop codon at 609372, 609615, 609642, 610402. Frameshift due to insertion around 610357. Nonsense mutation around 610402.", "psc": { "uniref90_id": "UniRef90_Q6GYZ7", "product": "Putative ATP-dependent DNA helicase", "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GYZ7", "UniRef:UniRef50_Q6GYZ7" ] }, "pscc": { "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7" ], "product": "Putative ATP-dependent DNA helicase" } }, "id": "EFPKPENHBP_1134", "locus": "GCA000186005_01106" }, { "type": "cds", "sequence": "contig_1", "start": 610627, "stop": 610809, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTCTAAGTGCCTTTTTTGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAGTATGAATGAAGTAAGTTTACTATTTTGA", "aa": "MLKIFKYMPARIKFFAFLSAFFAILQPFLSMLNPTITKQLITLIAKVQNGTSMNEVSLLF", "aa_hexdigest": "add83737200df4372843180430a2d7d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6888.356699999998, "isoelectric_point": 10.294907951354979 }, "id": "EFPKPENHBP_1135", "locus": "GCA000186005_01107" }, { "type": "cds", "sequence": "contig_1", "start": 610840, "stop": 611115, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGGGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGA", "aa": "MIYLIIMAIGFAFLLMIVAFLSAFFAARTCIYGTYYLRKNLFEHLLTLSRSNLEKYSVGSLITRFSNDLNKIKDGIFVVVRGLVVSPFFVV", "aa_hexdigest": "d5b6c3db62fa9e0a7ff9d94b6f109c51", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10382.362399999989, "isoelectric_point": 9.727586174011229 }, "id": "EFPKPENHBP_1136", "locus": "GCA000186005_01108" }, { "type": "cds", "sequence": "contig_1", "start": 611425, "stop": 611796, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTTGGCAATGTAGCTATTTTTGCCCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTTACAGCGAATTTAAGCATGATTTCAATGGGCGTTTTTAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAGTAACTAGTGGGGCTATTGAATTTAAGAATGTTAATTTTGCCTATCCTATCTATAAAAAAGGCAAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGCTAA", "aa": "MLFGNVAIFALVAYLIGSKGDDNVAKLIGDVSQFTANLSMISMGVFNTLFTINRLFRSSVSAKRYIELMNSKSEIPFVVSDKKVTSGAIEFKNVNFAYPIYKKGKLVDTKEALKNINLKIEAG", "aa_hexdigest": "ba353abb194dd39e922159023d3b3b8b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13549.713299999981, "isoelectric_point": 9.618570137023926 }, "id": "EFPKPENHBP_1137", "locus": "GCA000186005_01109" }, { "type": "cds", "sequence": "contig_1", "start": 611971, "stop": 612414, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF22", "UniRef:UniRef90_C4XF22" ], "nt": "ATGATTATTTCTGGCTCAATTAAAGAAAATATTACTTTTTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAGAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCTTTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAAGAAAACTTAAAAGATAGCACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAAATCAAATCTTTGTTCTTGATAAAGGACAAATTGTTGCTAGCGGTAAAAACAGTCAACTATTAAACACAAGTTCTATATATAAAGATATATGA", "aa": "MIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDDKYEARVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKENLKDSTLIVISQRINSIKDSNQIFVLDKGQIVASGKNSQLLNTSSIYKDI", "aa_hexdigest": "2e473a0cdb8e0dc021bafb3584ad4fad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 611800, "stop": 612444, "observations": { "mutation": [ 612412 ], "stop": [ 612412 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF22", "query_cov": 1.0, "subject_cov": 0.6869158878504673, "identity": 0.993, "score": 274.0, "evalue": 6.46e-92, "gene_start": 1, "gene_end": 147, "reference_start": 58, "reference_end": 204, "reference_sequence": "MGIIGKTGSGKSTLVKLLTHEYLLDKKDGEIIIDSHNINEIDTNNFYEKVTQVYQKPMIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDDKYEARVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKENLKDSTLIVISQRINSIKDSNQIFVLDKGQIVASGKHSQLLNTSSIYKDIWQSQEQKVGE" }, "paralog": false, "identity": 0.985981308411215, "score": 406.0, "evalue": 1.5e-146, "description": "Internal stop codon at 612412. Nonsense mutation around 612412.", "psc": { "uniref90_id": "UniRef90_C4XF22", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XF22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF22", "UniRef:UniRef50_C4XF22" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF22" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_1138", "locus": "GCA000186005_01110" }, { "type": "cds", "sequence": "contig_1", "start": 612612, "stop": 612911, "strand": "+", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23", "UniRef:UniRef90_C4XF23" ], "nt": "ATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATAAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAGCTTAGTGCTCGCCTTGGTCGGAATTTAAGGCTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGTTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCATTAACTAATGATATTCAAAATATAACTGAAGCAATCTGA", "aa": "MGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFIKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAI", "aa_hexdigest": "658ed711dbaeb7c5477cca72093968f8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 612411, "stop": 613007, "observations": { "mutation": [ 612438, 612549, 612606, 612909 ], "stop": [ 612438, 612549, 612606, 612909 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF23", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 184.0, "evalue": 1.08e-57, "gene_start": 1, "gene_end": 99, "reference_start": 68, "reference_end": 166, "reference_sequence": "MTISRTKSRWIMPRFSNLRFRQKGEKKISFKIFFKFLSNFQISKSVWIFTVLFGLLVASLTNIASWLMGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFIKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAIWDILISVISIIFEFSFITIMMFIYVPLLAAII" }, "paralog": false, "identity": 0.9797979797979798, "score": 362.0, "evalue": 4.39e-130, "description": "Internal stop codon at 612438, 612549, 612606, 612909. Nonsense mutation around 612438, 612549, 612606, 612909.", "psc": { "uniref90_id": "UniRef90_C4XF23", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF23", "UniRef:UniRef50_C4XF23" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "EFPKPENHBP_1139", "locus": "GCA000186005_01111" }, { "type": "cds", "sequence": "contig_1", "start": 612966, "stop": 613253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTTTATTTATGTTCCACTTTTAGCAGCTATTATTTTAGCAATTGTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGA", "aa": "MMFIYVPLLAAIILAIVPINASVIFLIIRSNQKFYIQQQERLGEFNGYLEEIMDAFPLVNLHQQKENVAKEFDKYSRALIKPDASATTRMNIVFP", "aa_hexdigest": "f145af2d28dff8627ca42a6722232759", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10962.781599999986, "isoelectric_point": 6.554865837097168 }, "id": "EFPKPENHBP_1140", "locus": "GCA000186005_01112" }, { "type": "cds", "sequence": "contig_1", "start": 613347, "stop": 613763, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGA", "aa": "MSAGVVMSFTLYLFRISDNISLLLEVINGIQHGLGSIVRINKILALVPQFDEVKLNELDYKTGKIEFKNVSFAYASKPDKLVLKNISFTIEKGKSLALVGHTGCGKTTIAKLLSKFYVPTSGDILIDGQSILKTKSKS", "aa_hexdigest": "cfd665868848676fff88c05bfc11cde4", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 52, "aa_cov": 0.37681159420289856, "hmm_cov": 1.0, "evalue": 1.182425888566479e-16, "score": 57.910335540771484, "start": 83, "stop": 134 } ], "seq_stats": { "molecular_weight": 15094.607899999983, "isoelectric_point": 9.547654914855958 }, "id": "EFPKPENHBP_1141", "locus": "GCA000186005_01113" }, { "type": "cds", "sequence": "contig_1", "start": 613911, "stop": 614276, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter, ATP-binding/permease protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139QXF8" ], "nt": "ATGCCTGAAAAATTTGATACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGGCAAAAACAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAATATTGACACAATAACAGAATTAAAAGTGCAACATGCAATGAAATACTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAGTACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGACCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTAAATGATACAAAAATTGATTAG", "aa": "MPEKFDTVLKSNGANLSEGQKQLIAISRSLIASKPISILDEATSNIDTITELKVQHAMKYLMKDRSMLIIAHRLSTIKNVDEILVINEGEIIERGNHDQLMKQNGHYKKIYSLGLNDTKID", "aa_hexdigest": "17e9f0fadd868aeee6f0f1183666f8c9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A139QXF8", "query_cov": 0.9256198347107438, "subject_cov": 0.9491525423728814, "identity": 0.563, "score": 132.0, "evalue": 7.46e-38, "valid": false, "product": "ABC transporter, ATP-binding/permease protein", "uniref50_id": "UniRef50_A0A139QXF8" }, "pscc": { "uniref50_id": "UniRef50_A0A139QXF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139QXF8" ], "product": "ABC transporter, ATP-binding/permease protein" }, "genes": [], "id": "EFPKPENHBP_1142", "locus": "GCA000186005_01114" }, { "type": "cds", "sequence": "contig_1", "start": 614313, "stop": 615029, "strand": "-", "frame": 2, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_UPI0001E32E7D", "UniRef:UniRef90_UPI0001E32E7D" ], "nt": "ATGAAAGAAGCCAACATTAAAACTAATTATTTGGTAAATGAGTTCAAAAGAACTTATTTAATAGATATAGTTGAAAAAAATAAAATAGAAGATATTGATAACTTAGATGACATAATCAATATTTTAGCTTCTAATATAGGTTCATACACTAATCCTTATAAATTATCATTAACTTTTAAATCAATTAAGAAAACAAACATTTCTGATGCAACAATTAGAAAATATATTGAAAATTTAAAAAATGCTTTCATAATTAATGAAGTCAAAAAATATGATATTAAAGGTAAAAAGTATATTAATGCTTCATACAAATATTATTTTGAAGATATAGGTTTAAGAAATGCTAAAATCAATTTTGCACAAAATGAAGAAAATCATTTAATGGAAAATATCATTTATAATGAATTGCTTTTAAGAGGTTACAATGTTAATGTTGGAATAGTAGAAATAAGACCTAATGCTAATCAGAAAGTTCAATTAGAAGTAGATTTTATTGCTCAAAAAGGTAATCAAAAAGTTTATATTCAATCTTGTTTAAATATGTATGATCAAAATAAAGAAATTCAAGAAAAAAGACCTTTTAAAAACATCAATGATAATTTTAAAAAAATTATTATTACTAAAGATGACCCTATTTTTGATACTGATACTAATGGTTATAAAATTTATGGAATCAAACAATTTTTACTTGATTTAGATATTCTAAATAAAAATTAA", "aa": "MKEANIKTNYLVNEFKRTYLIDIVEKNKIEDIDNLDDIINILASNIGSYTNPYKLSLTFKSIKKTNISDATIRKYIENLKNAFIINEVKKYDIKGKKYINASYKYYFEDIGLRNAKINFAQNEENHLMENIIYNELLLRGYNVNVGIVEIRPNANQKVQLEVDFIAQKGNQKVYIQSCLNMYDQNKEIQEKRPFKNINDNFKKIIITKDDPIFDTDTNGYKIYGIKQFLLDLDILNKN", "aa_hexdigest": "d1d0d9a95fd08e1e89f844b12b228ea6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E7D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.971, "score": 445.0, "evalue": 9.39e-158, "valid": true, "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI0001E32E7D", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E7D" ], "product": "DUF4143 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1143", "locus": "GCA000186005_01115" }, { "type": "cds", "sequence": "contig_1", "start": 615072, "stop": 615563, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTAAACGGGATATATATTTAGAAAAACTTAAAGAGATGATTGATATACCTCTTATTAAAATAATTTCAGGCTTAAGAAGAAGTGGAAAGTCGTTTTTGTTATTTAATTTATTCAAGGAATATTTAATAAATGAGTTAAAAGTTGATCAAAATCATATTATTGAAATTAATTTAGAAAATCAAGAAAATCATTCTTATTGTGATCCTAATAATTTTTTGGAATTTATAAAAAGTAAAATTAAAGATCGAAAAAAGTATTTTCTTTTAATTGATGAAGTGCAACTTTTAAAAGATTTTGTGCCTGTTTTAAATCAACTTAATAATAATCAACAACTTAATGTTTTTGTCACTGGCTCCAATTCTAAATTCTTAATTAATGATGTAGTTACAGAATTTAGAGGTAGATCGATTTCGCTTCATGTTTTTCCCTTATCTTTCTATGAAATAGCCTCACACAAAGAATTTAAAAACCTTGATGAGTTATGA", "aa": "MVIKRDIYLEKLKEMIDIPLIKIISGLRRSGKSFLLFNLFKEYLINELKVDQNHIIEINLENQENHSYCDPNNFLEFIKSKIKDRKKYFLLIDEVQLLKDFVPVLNQLNNNQQLNVFVTGSNSKFLINDVVTEFRGRSISLHVFPLSFYEIASHKEFKNLDEL", "aa_hexdigest": "060299fe7312b29129bebbc3566ea142", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19323.2052, "isoelectric_point": 6.912951469421387 }, "id": "EFPKPENHBP_1144", "locus": "GCA000186005_01116" }, { "type": "tRNA", "sequence": "contig_1", "start": 615739, "stop": 615823, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(aag)", "amino_acid": "Leu", "anti_codon": "aag", "score": 71.4, "nt": "GCCCGAGTGGTGAAATTGGCAAACACGCTAGACTAAGGATCTAGTGCTTAACGGCTTGCAGGTTCAAGTCCTGTCTCGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 615787, 615789 ], "id": "EFPKPENHBP_32", "locus": "GCA000186005_01117" }, { "type": "cds", "sequence": "contig_1", "start": 615886, "stop": 616284, "strand": "-", "frame": 1, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26", "UniRef:UniRef90_C4XF26" ], "nt": "TTGAAATATAGTGATTTAGGTCAAAAAACTAATGTAACTAAAATTTTAACTTTTGGTAAGGATGAAATAGCTACTAAAAATTTTAAGAACTTTTTAGCTAAAAAATATCCTTCACTTGCATTTCATATAGTTTCAAAAGGTTACTCAATTGAAATCACACATAAAAATGCAACCAAAGGTAAAGGTGCAGCATTTGTTTGTGACTTATTAAAAATAGATCCTAAAAACGCTGCTCACATTGGTGATAGCGGAAATGATTTAAATGCTTTACCTGAGATAGGAACATTCATTGCTATGGAAAATGCAATGGATGAGGTAAAAGAAGTAGCACATTATATAGGGCCTTCTTTTGAAAATAGTGGAATAGCCAAGCTATTTAAAGATTTAAAAGAAATATAA", "aa": "MKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI", "aa_hexdigest": "0c273b52d5694e192a0637ad9698282b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 615886, "stop": 616584, "observations": { "mutation": [ 616305 ], "stop": [ 616305 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF26", "query_cov": 1.0, "subject_cov": 0.43137254901960786, "identity": 0.992, "score": 265.0, "evalue": 3.24e-87, "gene_start": 1, "gene_end": 132, "reference_start": 175, "reference_end": 306, "reference_sequence": "MNIFYQYIYKTALLVNDWKLGNYRYIFFVSYIKSLYIIKIMKKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRPWTAKLEKLKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI" }, "paralog": false, "identity": 0.9956896551724138, "score": 458.0, "evalue": 6.67e-166, "description": "Internal stop codon at 616305. Nonsense mutation around 616305.", "psc": { "uniref90_id": "UniRef90_C4XF26", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF26", "UniRef:UniRef50_C4XF26" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26" ], "product": "COF family HAD hydrolase protein" } }, "id": "EFPKPENHBP_1145", "locus": "GCA000186005_01118" }, { "type": "cds", "sequence": "contig_1", "start": 616303, "stop": 616686, "strand": "-", "frame": 1, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "nt": "ATGAAAAAAGAAATTCAAGCTTATTTTATAGATTTAGATGGAACATTATTAGACTTACCAGAAGGTGAAGAAACTCTTTCAAGTGCTAATGTTAAAGCTGTTGTTAAATTAAATAAAACTAAACCAGTTATTATTTCAACAGGTAGAAGTAATAGCGAATTTGTTTTATCCTTAGTTAAATTGTTAAAGTGCCCTTATGCCATTTGTCAAAATGGAGCTTTAATAGTTGATAAGAAAAATAAAGTTTTGGCTAGATATGAGATGGAACAAAATCAAGTTGATGAAATAGTTAAGCTTTTAAAAGAAGAAGAAATGTTTTTTATTTTTAATGGCGAAAATAAAATCTATTGTAACTATGAATCAAGTGCCTACATGCGCCCTTGA", "aa": "MKKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRP", "aa_hexdigest": "e43ef937a803625d914152e38a7e467f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KXX5", "query_cov": 0.8110236220472441, "subject_cov": 0.9716981132075472, "identity": 0.6990000000000001, "score": 153.0, "evalue": 3.94e-46, "valid": false, "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_A0A3P8KXX5" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "product": "COF family HAD hydrolase protein" }, "genes": [], "id": "EFPKPENHBP_1146", "locus": "GCA000186005_01119" }, { "type": "cds", "sequence": "contig_1", "start": 617122, "stop": 617535, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF27", "UniRef:UniRef90_C4XF27" ], "nt": "TTGTACAGTTTTGTAAAAAATAAATCTTTAATTAAAAACATTGAATCATTTGGTTCAACTTTGATTAAAGATTTGATCAAAAGAATCAACAAACAAAAATATATTAGAGTTCAAAAAGAACTATTTGGCAGTGGAAGCGAAGAATTAATAGTTCAAAATGATAAAGGCGTAATTATAATAGATTATAACCTTATTGTTTATGCTTTTTTTGAAGGCAACAGTTTATATGACTGTAAAAAGATAAGAGAATTTGTAAAAAAGAATTTTGATGAATCACTAACTCAGCTTGGCTGCGAAATATCAAAAGATCATTCTCTTTCATTAACAGCTGAAAATCTAACAATTGATGATTATAAAGATACTCCTTTTATAATTAATTTAAATATTGTTTTTGAATTTGGACCAGAATATTGA", "aa": "MYSFVKNKSLIKNIESFGSTLIKDLIKRINKQKYIRVQKELFGSGSEELIVQNDKGVIIIDYNLIVYAFFEGNSLYDCKKIREFVKKNFDESLTQLGCEISKDHSLSLTAENLTIDDYKDTPFIINLNIVFEFGPEY", "aa_hexdigest": "73a70c9b9263c566981e8471c4803850", "start_type": "TTG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 616861, "stop": 617535, "observations": { "mutation": [ 616932, 617004, 617070, 617076, 617124 ], "stop": [ 616932, 617004, 617070, 617076, 617124 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF27", "query_cov": 1.0, "subject_cov": 0.6116071428571429, "identity": 1.0, "score": 273.0, "evalue": 8.54e-92, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MYSFVKNKSLIKNIESFGSTLIKDLIKRINKQKYIRVQKELFGSGSEELIVQNDKGVIIIDYNLIVYAFFEGNSLYDCKKIREFVKKNFDESLTQLGCEISKDHSLSLTAENLTIDDYKDTPFIINLNIVFEFGPEYWIQLAHFKKSDAKKERWFWYNVPNTNSLMKRVEECKECDLWKDVQNIYLEKMNDFSKIFNRNADWFYCYAAAITEVENKNSDYDGED" }, "paralog": false, "identity": 0.9732142857142857, "score": 434.0, "evalue": 2.31e-157, "description": "Internal stop codon at 616932, 617004, 617070, 617076, 617124. Nonsense mutation around 616932, 617004, 617070, 617076, 617124.", "psc": { "uniref90_id": "UniRef90_C4XF27", "uniref50_id": "UniRef50_C4XF27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF27", "UniRef:UniRef50_C4XF27" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF27" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15895.071899999988, "isoelectric_point": 5.480665779113769 }, "id": "EFPKPENHBP_1147", "locus": "GCA000186005_01120" }, { "type": "cds", "sequence": "contig_1", "start": 617839, "stop": 618180, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATATTTTAGTATTCAAAAATAAATATGTTGATTATCAAGATACTGAAAATTACAATTTTGAAACTGTTTCAAATAATAGTTTCCAAAAGTTGGCAAAAGAAAAATATAATGAATTTTTAGCTAAATTTCAAGATTTAAAAATTAATAAAATTTTTGTTAAAGATCTTCAACCTTTTTATTCAAGTAATGACAGTGAAACTATAAAGCTTTTAGAATTTTATATTTATCTTTATAAAGCACTTTATTGTACTTTATCAAACTTTATATCATTAGCGTATAGTAAAGAAGATGCTTTAAATAAATGTATCAACATTAAAGATTATTGTCACGAGTATTGA", "aa": "MDILVFKNKYVDYQDTENYNFETVSNNSFQKLAKEKYNEFLAKFQDLKINKIFVKDLQPFYSSNDSETIKLLEFYIYLYKALYCTLSNFISLAYSKEDALNKCINIKDYCHEY", "aa_hexdigest": "47f00f766684777b481db34dbd78112b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13607.305399999987, "isoelectric_point": 5.3758546829223635 }, "id": "EFPKPENHBP_1148", "locus": "GCA000186005_01121" }, { "type": "cds", "sequence": "contig_1", "start": 618460, "stop": 618735, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGATATAACAATTACACAAATGCAATTTAATGAATATTTATATGATTATAGTCAAGATCAAGAAAAGAAAATTCGTTTAGATGATTGGACTGCTTTTGATGAATATTATTATGAATTATTAAAAAAATATATCAAAGAGAAAGTTACTAATACTAATGAGAAAATAAGAATTTATTTCTTGCCTCGTTATGATAATAAATATTATGCTTTTAGACTTAAATATGAAAGTGAAAAAGAAGCTGAAAAAGAATTAGGCAAAGAAGAATGA", "aa": "MKLDITITQMQFNEYLYDYSQDQEKKIRLDDWTAFDEYYYELLKKYIKEKVTNTNEKIRIYFLPRYDNKYYAFRLKYESEKEAEKELGKEE", "aa_hexdigest": "d4bf6510253b5d325f7b78432f4ea127", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11487.806199999992, "isoelectric_point": 5.140995979309081 }, "id": "EFPKPENHBP_1149", "locus": "GCA000186005_01122" }, { "type": "cds", "sequence": "contig_1", "start": 618915, "stop": 619136, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC57", "UniRef:UniRef90_A0AB32XC57" ], "nt": "ATGTTCAAAAATGTTAATATCGATTTTCTCCCTTATTACATTGTTGATGGCCAAGGTTATAAGAGCTTAATTAGAAAATATCAAGTAATAACTTCTTCTAAGAATGCAAAATTTGAAATAGTCTATGGTTACTTATTACTAGTAGCAAGCATTAACTTAATTTTATTAGATTATTTAAATCAAAATAGTATTAATAATTCTGCTTTTGAATATGAAGTTTAG", "aa": "MFKNVNIDFLPYYIVDGQGYKSLIRKYQVITSSKNAKFEIVYGYLLLVASINLILLDYLNQNSINNSAFEYEV", "aa_hexdigest": "ce759fb92ec6a27806a2e278e9e0d2d9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 618915, "stop": 619259, "observations": { "mutation": [ 619157 ], "stop": [ 619157 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XC57", "query_cov": 1.0, "subject_cov": 0.6403508771929824, "identity": 1.0, "score": 143.0, "evalue": 7.36e-43, "gene_start": 1, "gene_end": 73, "reference_start": 42, "reference_end": 114, "reference_sequence": "MNNEFTNFIFKEKIITNLYDNDNKLTKELKNKNIWDILNYKMFKNVNIDFLPYYIVDGQGYKSLIRKYQVITSSKNAKFEIVYGYLLLVASINLILLDYLNQNSINNSAFEYEV" }, "paralog": false, "identity": 0.9824561403508771, "score": 224.0, "evalue": 1.78e-77, "description": "Internal stop codon at 619157. Nonsense mutation around 619157.", "psc": { "uniref90_id": "UniRef90_A0AB32XC57", "uniref50_id": "UniRef50_A0AB32XC57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XC57", "UniRef:UniRef50_A0AB32XC57" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XC57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC57" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8493.687599999996, "isoelectric_point": 6.058888816833496 }, "id": "EFPKPENHBP_1150", "locus": "GCA000186005_01123" }, { "type": "cds", "sequence": "contig_1", "start": 619632, "stop": 619769, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGGATTAACAAGAGAAATTTATACTAGAAAAGGTAAAGTTAATAGTAGTGGAGCTTATCAAGGACCTAAATATTTCCAATTTAATGGTAAAAGGCTTACTGAATTGCCAATAATTGAAGAATGCCAATCTTAA", "aa": "MSGLTREIYTRKGKVNSSGAYQGPKYFQFNGKRLTELPIIEECQS", "aa_hexdigest": "c4eab319b03caa553623bfe6a41946fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5126.778799999998, "isoelectric_point": 9.358891487121582 }, "id": "EFPKPENHBP_1151", "locus": "GCA000186005_01124" }, { "type": "cds", "sequence": "contig_1", "start": 619773, "stop": 620267, "strand": "-", "frame": 2, "gene": null, "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF30", "UniRef:UniRef90_C4XF30" ], "nt": "ATGCCAGAAAAAGGATATGTTTATGTTTTAACTAACCCAAGTTTTAGAGATGATTGGGTTAAAATTGGTAAAAGCAGTAGAATGCCTGAAGTTAGAGGTCGTGAATTATATAATACAGCTGTTCCACTTCCTTATGAAATCTATGCCACTTTACAAACTGAAAAATATAATGAAGTTGAAAAAATGATTCACAAAAGTATTGATCGAATTTCAAAATTAAGAATAAACAAAAATAGAGAATTTTTTAATATACCACCTGAAAAAGCTTATGACATTTTACTTGATATTAAGGAATTATTAGGAAATGAAGCTAAATTAGAATTAATGGGTGATAATGTCGAAGTACATACTAAAGAAAAAACAAAAAGAGGTGAACCATTTGACTTTTATAGTAGAGGTTTAAAAGATGGAGATGTTGTTTCATTCATAGAAAATTCAAACATAAAAGCAATTGTTGAATCAAATAAAACTGTATATTTTGAAAATCAAAGTTGA", "aa": "MPEKGYVYVLTNPSFRDDWVKIGKSSRMPEVRGRELYNTAVPLPYEIYATLQTEKYNEVEKMIHKSIDRISKLRINKNREFFNIPPEKAYDILLDIKELLGNEAKLELMGDNVEVHTKEKTKRGEPFDFYSRGLKDGDVVSFIENSNIKAIVESNKTVYFENQS", "aa_hexdigest": "af3ece7a340f895bec58c09b9b2a5269", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 619632, "stop": 620267, "observations": { "mutation": [ 619775 ], "stop": [ 619775 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF30", "query_cov": 1.0, "subject_cov": 0.7772511848341233, "identity": 1.0, "score": 328.0, "evalue": 5.47e-113, "gene_start": 1, "gene_end": 164, "reference_start": 1, "reference_end": 164, "reference_sequence": "MPEKGYVYVLTNPSFRDDWVKIGKSSRMPEVRGRELYNTAVPLPYEIYATLQTEKYNEVEKMIHKSIDRISKLRINKNREFFNIPPEKAYDILLDIKELLGNEAKLELMGDNVEVHTKEKTKRGEPFDFYSRGLKDGDVVSFIENSNIKAIVESNKTVYFENQSWSLSGLTREIYTRKGKVNSSGAYQGPKYFQFNGKRLTELPIIEECQS" }, "paralog": false, "identity": 0.995260663507109, "score": 422.0, "evalue": 1.04e-152, "description": "Internal stop codon at 619775. Nonsense mutation around 619775.", "psc": { "uniref90_id": "UniRef90_C4XF30", "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein", "uniref50_id": "UniRef50_C4XF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF30", "UniRef:UniRef50_C4XF30" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF30" ], "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein" } }, "id": "EFPKPENHBP_1152", "locus": "GCA000186005_01125" }, { "type": "cds", "sequence": "contig_1", "start": 620751, "stop": 621341, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB32XBZ4", "UniRef:UniRef90_A0AB32XBZ4", "PFAM:PF01420.25" ], "nt": "ATGCAAAAAACTTTAAAATGCGCAATTATAAATAATGAATTAAATGGTTCATTATTTTCAACTGGTTTTTATGGTTTTAAATCTATTTATAATAAAATAAAATATTTAAAACTGTTTATAGAGTCTCCTTATTATCAAATTTTAAAAGATTCTTTTTGCAGTGGAGTAACTCAAAAATCAATTAATGATGAAAAATTGCTAAATATTTTAATTGCTATTCCGCCAATAAATGAACAAGAAAAAATCATTAACAAATTAATATCATTAGATAAATTTATGAAGAAATATTTAGAAAAAGAAAATCAATTATTCAAACTAGATTCTGAAATTAAAGACAAATTACAAAAATCTATCTTACAATATGCAATTCAAGGAAAATTAGTTAAACAAGATCCTAATGATGAACCAGCATCAAAATTATTAGAAGCTATACAAATTGAAAAAAACAAACTAATTAAAGAAGGAAAAATAAAGAAAGATAAACATGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGCTCAAAAGTTATAAATATAACAAACGAAATACCTTTTGAAATACCAAAAAATTGA", "aa": "MQKTLKCAIINNELNGSLFSTGFYGFKSIYNKIKYLKLFIESPYYQILKDSFCSGVTQKSINDEKLLNILIAIPPINEQEKIINKLISLDKFMKKYLEKENQLFKLDSEIKDKLQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNKLIKEGKIKKDKHESFIFQGEDKNYYEKIGSKVINITNEIPFEIPKN", "aa_hexdigest": "16d0c726a0dfcf50ecd3d95c6180974d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 620592, "stop": 621644, "observations": { "mutation": [ 621339 ], "stop": [ 621339 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBZ4", "query_cov": 1.0, "subject_cov": 0.49370277078085645, "identity": 1.0, "score": 374.0, "evalue": 9.13e-128, "gene_start": 1, "gene_end": 196, "reference_start": 54, "reference_end": 249, "reference_sequence": "METGVKKFDGLKKYYSTGSITKDNYVPEGEYSYNSKPSRANRIHFLGELIDAKMQKTLKCAIINNELNGSLFSTGFYGFKSIYNKIKYLKLFIESPYYQILKDSFCSGVTQKSINDEKLLNILIAIPPINEQEKIINKLISLDKFMKKYLEKENQLFKLDSEIKDKLQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNKLIKEGKIKKDKHESFIFQGEDKNYYEKIGSKVINITNEIPFEIPKNWVIVKISNISFRIDKKNIIIKTKQILSTGKYPIITQGQKFIEGYTNNVNNIFKVKEPIIIFGDHTKTTKFVDFNFVPGGDGTVFLKPLKINPLFFYYLVNYLSKKIRNRGYARHYILLKKEIIPIPNINEQNQIVSKIKKVFYFINCL" }, "paralog": false, "identity": 0.9942857142857143, "score": 694.0, "evalue": 2.16e-256, "description": "Internal stop codon at 621339. Nonsense mutation around 621339.", "psc": { "uniref90_id": "UniRef90_A0AB32XBZ4", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB32XBZ4", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBZ4", "EC:3.1.21.3", "UniRef:UniRef50_A0AB32XBZ4", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBZ4" ] } }, "hypothetical": true, "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 80, "aa_cov": 0.3979591836734694, "hmm_cov": 0.9625, "evalue": 1.2750718961256362e-07, "score": 28.327777862548828, "start": 16, "stop": 93 } ], "seq_stats": { "molecular_weight": 22789.326900000015, "isoelectric_point": 9.118166542053224 }, "id": "EFPKPENHBP_1153", "locus": "GCA000186005_01126" }, { "type": "cds", "sequence": "contig_1", "start": 621996, "stop": 622508, "strand": "-", "frame": 2, "gene": null, "product": "Type I restriction-modification system, S subunit", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD44", "UniRef:UniRef100_A0AB32XBX2", "UniRef:UniRef50_A0AB32XBX2", "UniRef:UniRef90_A0AB32XBX2" ], "nt": "ATGTTAAATTTGGTTAAAATTATTTCAGGAATATCTTATGACAAAAACGATATTTTAACTAAAATGGATAAAAACTACATTCGTATAATTCGAAGTGGAAATATTCAAAACAGCAGATTAATATTATTCGATGATGACATTTTTTTACCTGTTTTTTATAAAAACAACATAAAAATGTTACATTACAATGACATTGTTATTATGGCTTCGACAGGAAGTAAGAACTTAATAGGAAAGCCAGCATTTGTCGAAGAACAACTTGATAATGTTTATATTGGAGCTTTCTTAAGAATAATAAGACCTAATATTAATAATATTTTTGATTATTTAAAATTAATTTTTATGAGCGAATATTATAGAAGTGAAATAAGAAAAAATGTTAATGGAACAAATATTAATAATGTTAATTCAAATATATTATTAAATATGTTAATTCCAATTCCATCAATAAAAAATGAAAGAAAAATTTCAAAAAAAATTTACCAAGTATTAAATATATTAACATTAATGTAA", "aa": "MLNLVKIISGISYDKNDILTKMDKNYIRIIRSGNIQNSRLILFDDDIFLPVFYKNNIKMLHYNDIVIMASTGSKNLIGKPAFVEEQLDNVYIGAFLRIIRPNINNIFDYLKLIFMSEYYRSEIRKNVNGTNINNVNSNILLNMLIPIPSIKNERKISKKIYQVLNILTLM", "aa_hexdigest": "61e4e523ab494cad50bf0d5fc1aefe9e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F5AD44", "uniref100_id": "UniRef100_A0AB32XBX2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD44", "UniRef:UniRef100_A0AB32XBX2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBX2", "uniref90_id": "UniRef90_A0AB32XBX2" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBX2", "product": "Type I restriction-modification system, S subunit", "uniref50_id": "UniRef50_A0AB32XBX2" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBX2" ], "product": "Type I restriction-modification system, S subunit" }, "genes": [], "id": "EFPKPENHBP_1154", "locus": "GCA000186005_01127" }, { "type": "cds", "sequence": "contig_1", "start": 622642, "stop": 622902, "strand": "-", "frame": 1, "gene": null, "product": "Tyr recombinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ3VAA0" ], "nt": "TTGAATAGATTCGGAAAAGCAATAACAACTAGAGGCATTTCTCAACAATTAAAAAAATATGCTTTAAAATTTGGCATAAACCCAAAAGTTGTTTATCCTCATTCATTTAGACATAGATTCGCTAAAAACTTTTTAGAAAAATATAACGATATATCTTTTTTAGCTGACTTGATGGGTCATGAATCAATTGAAACAACAAGAATTTATTTAAGAAAAACTTCAAATGAACAACAAGAAATTGTAGACAAAATTATCACTTGA", "aa": "MNRFGKAITTRGISQQLKKYALKFGINPKVVYPHSFRHRFAKNFLEKYNDISFLADLMGHESIETTRIYLRKTSNEQQEIVDKIIT", "aa_hexdigest": "8eda6ee2c88ef8db3dfffa0b89f91c41", "start_type": "TTG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0026E9C367", "query_cov": 1.0, "subject_cov": 0.8958333333333334, "identity": 0.826, "score": 148.0, "evalue": 7.61e-45, "valid": false, "product": "tyrosine-type recombinase/integrase", "uniref50_id": "UniRef50_A0AAJ3VAA0" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ3VAA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ3VAA0" ], "product": "Tyr recombinase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1155", "locus": "GCA000186005_01128" }, { "type": "cds", "sequence": "contig_1", "start": 622939, "stop": 623064, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTGAACTTATAAAAATTAAAGTCGAACATGTAAAACTTGGGTATATTGATATTTATAGCAAAGGTGGAAAAGCTAGAAGAATTTACATTCCTTTAAAATTAAAAAAAGAAGCAATCATTTGA", "aa": "MSELIKIKVEHVKLGYIDIYSKGGKARRIYIPLKLKKEAII", "aa_hexdigest": "7eae79e4272a789459381c93ae555678", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4756.782099999999, "isoelectric_point": 9.918412590026854 }, "id": "EFPKPENHBP_1156", "locus": "GCA000186005_01129" }, { "type": "cds", "sequence": "contig_1", "start": 623104, "stop": 623400, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027349DB5" ], "nt": "TTGACTAAAAATACTATAAATGCTTACAAATCGGCAATTAAACTATTTAATTTAAAATATGAAGAAATTAATAAAAAAAATTTATTGTTATTCAAAACATATCTTATAGAAAACTATAAACCTAATTCAGTTAATTTAAAAATCCAAGCTTTAAATAGGTATCTTGAATTTATAAAAAAAGATACTTTAAAATTAAAATTTATAAAAGTTCAACAAAAAACTTTTATTGACAACGTAATTAGTGATGCTGATTACGTTTTTTTAAAAAGAAAACTAAAAAAAGAATCACTCGATTGA", "aa": "MTKNTINAYKSAIKLFNLKYEEINKKNLLLFKTYLIENYKPNSVNLKIQALNRYLEFIKKDTLKLKFIKVQQKTFIDNVISDADYVFLKRKLKKESLD", "aa_hexdigest": "89a3b79f51d4c3f29870c12a2cf8755a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027349DB5", "query_cov": 0.9387755102040817, "subject_cov": 0.8363636363636363, "identity": 0.5429999999999999, "score": 103.0, "evalue": 8.7e-27, "valid": false, "uniref50_id": "UniRef50_UPI0027349DB5" }, "pscc": { "uniref50_id": "UniRef50_UPI0027349DB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027349DB5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11739.763299999986, "isoelectric_point": 9.823063850402832 }, "id": "EFPKPENHBP_1157", "locus": "GCA000186005_01130" }, { "type": "cds", "sequence": "contig_1", "start": 623889, "stop": 624038, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTTTATAAATCAAGTATTTATCAAACATTAGTTAAAAATAATTCAAGAAGTGCGCAAGCCGGATTTAATAAAGACGATTTAAAAAATCTTTTTATGCCAATTCCTTCTTTAAATGAATCGTCAAGAATTGTAAGTAAATGA", "aa": "MFIFYKSSIYQTLVKNNSRSAQAGFNKDDLKNLFMPIPSLNESSRIVSK", "aa_hexdigest": "7a5dbe482dff17f5a06cda5d26a867b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5629.4245999999985, "isoelectric_point": 9.871673011779784 }, "id": "EFPKPENHBP_1158", "locus": "GCA000186005_01131" }, { "type": "cds", "sequence": "contig_1", "start": 624120, "stop": 624371, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAATCTATTTTGCAATATGCAATTCAGGGTAAATTAGTTAAACAAGATCCCAATGACGAACCAGCTTCTAAATTATTAGAAGCTATACAAATTGAAAAAAATGAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAATAAATTGA", "aa": "MQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNELIKEGKIKKDKQESFIFQGEDKNYYEKIGSKVINITNEIPFEIPIN", "aa_hexdigest": "b70ea2aac4327a7643c24e52d1c19ceb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ED51773", "query_cov": 0.9759036144578314, "subject_cov": 0.9418604651162791, "identity": 0.593, "score": 91.3, "evalue": 1.82e-22, "valid": false, "uniref50_id": "UniRef50_K1U594" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9593.913399999992, "isoelectric_point": 5.470434761047363 }, "id": "EFPKPENHBP_1159", "locus": "GCA000186005_01132" }, { "type": "cds", "sequence": "contig_1", "start": 624562, "stop": 624873, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_013526928.1", "SO:0001217", "UniParc:UPI0001F5AD47", "UniRef:UniRef100_C4XF32", "UniRef:UniRef50_C4XF32", "UniRef:UniRef90_C4XF32" ], "nt": "ATGTCCAAAAAAGGAACTTTATTATTGAGTTTTAAATTAACAATAGGTAAAACATCAATAATTAATCAAGATTCAGTTCATAATGAAGCAATTGTTTCAATAAATTTTTACAAAGATAATAATATAACAAAAATGTTTCTATTAATATTTTTAGGATTATTGATCAATAATTGTGAAAAAATTAATGCTATCAAAGGAAAAACATTAAATAAGGAAAAATTGCAAAAAATGTTAATTCCTATACCCCCTATCAAAAATCAAAATAATATTTTATTAATAACTAATAAAATTATCGATCTTTTTAAGTTTTAA", "aa": "MSKKGTLLLSFKLTIGKTSIINQDSVHNEAIVSINFYKDNNITKMFLLIFLGLLINNCEKINAIKGKTLNKEKLQKMLIPIPPIKNQNNILLITNKIIDLFKF", "aa_hexdigest": "b915a3cf47e17c1eb5e0dca93857817e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5AD47", "ncbi_nrp_id": "WP_013526928.1", "uniref100_id": "UniRef100_C4XF32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD47", "RefSeq:WP_013526928.1", "UniRef:UniRef100_C4XF32" ] }, "ips": { "uniref100_id": "UniRef100_C4XF32", "uniref90_id": "UniRef90_C4XF32" }, "psc": { "uniref90_id": "UniRef90_C4XF32", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XF32", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_C4XF32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF32" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1160", "locus": "GCA000186005_01133" }, { "type": "cds", "sequence": "contig_1", "start": 624835, "stop": 625587, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_013526929.1", "SO:0001217", "UniParc:UPI0001F5AD48", "UniRef:UniRef100_A0AB32XCC0", "UniRef:UniRef50_A0AB32XCC0", "UniRef:UniRef90_A0AB32XCC0", "PFAM:PF01420.25" ], "nt": "GTGTTATATAAAAAAACAGAAAATCAACTATTTAAACTTGATTCCGAAATTAAAGATAAATTGCAAAAATCTATTTTGCAATATGCAATTCAGGGAAAATTGGTTAAACAAGATCCTAATGATGAACCAGCTTCTAAGCTTTTAGAAGCTATACAAATTGAAAAAAATAAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTAATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAAAAAAATGGGCTTGGGTTAGACAAAAAAACATTTTAAAATTGACAAAAAATGAAGCTTCTAAAAATGGAAATTACCCTTATCTAGAAGCAAAGGTACTAAGAAAAATTATTAAACCGAAAATTATTAATAATGGGGTTTTAATAAATAAAGGAGATATTGTTATTTTGGTTGATGGTGAGAATTCAGGTGAAACATTTGTTCTAGACCAAACTGGTTATATGGGTAGTACATTTAAACTATTAAAAATAAATAACAAAATAGATCAGGAATATGTTTTAATGTTATTAAAATTTTATAAAGAATTATTCAAGAAAAATAAAAAAGGAGCTGCAATACCACATTTAAATATAGACATTTTCAATAATTTACTTTTGGCTATTCCAAACATAAAAGAACAAAAAGAAATCATTTTAAAACTTAAAAAGATCGATAATTTTATTAGTTATTAA", "aa": "MLYKKTENQLFKLDSEIKDKLQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNKLIKEGKIKKDKQESLIFQGEDKNYYEKIGSKVINITNEIPFEIPKKWAWVRQKNILKLTKNEASKNGNYPYLEAKVLRKIIKPKIINNGVLINKGDIVILVDGENSGETFVLDQTGYMGSTFKLLKINNKIDQEYVLMLLKFYKELFKKNKKGAAIPHLNIDIFNNLLLAIPNIKEQKEIILKLKKIDNFISY", "aa_hexdigest": "1eaa8f8550d78b759c87cbdee01c986a", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5AD48", "ncbi_nrp_id": "WP_013526929.1", "uniref100_id": "UniRef100_A0AB32XCC0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD48", "RefSeq:WP_013526929.1", "UniRef:UniRef100_A0AB32XCC0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCC0", "uniref90_id": "UniRef90_A0AB32XCC0" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCC0", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB32XCC0", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCC0" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 156, "aa_cov": 0.588, "hmm_cov": 0.9935897435897436, "evalue": 1.5772406206003147e-15, "score": 54.05693054199219, "start": 102, "stop": 248 } ], "seq_stats": { "molecular_weight": 28997.783600000053, "isoelectric_point": 9.543915748596191 }, "id": "EFPKPENHBP_1161", "locus": "GCA000186005_01134" }, { "type": "cds", "sequence": "contig_1", "start": 625986, "stop": 626840, "strand": "+", "frame": 3, "gene": null, "product": "SF4 helicase domain-containing protein", "db_xrefs": [ "EC:5.6.2.3", "KEGG:K02314", "RefSeq:WP_013526930.1", "SO:0001217", "UniParc:UPI00003B49A9", "UniRef:UniRef100_C4XDP9", "UniRef:UniRef50_C4XDP9", "UniRef:UniRef90_C4XDP9" ], "nt": "ATGCTACTCACTACAAAACAGTTACAGGAATTTTACAAAATTGAAATGGAAAATATTGCAAAAAATAAATCTCTATTAACTAACTTCAATATCATTGATAATTATGCAAATGGTTTAAAACCTAAACAACTTTATTTTTTAGCTGCAAGACCAGGCGTTGGAAAAACTTCTTTTGCTCTTAACATTATTTTTAATGCTTTGGAAAATTTGAAAGAAGATGAATGCATTGTTTTGTTTAGTCTTGAAATGGACTCAATTGAAATATATTCAAAACTTTTAGCTCTTGCCAATTCTTGTTCACTTAAAGAAGTTGAAACAAATTCTAAGAAAATAGATTTTGAAGATTTAAACAAAAAGAACCTTTTTATATTTGATAGATATAGAAACAATTTTAATAATGAAAAATTAAGTGAAGTGACTCCTGACATTATTTATAAAAGTTTGAATTCTATTGGTTTAAAGATCAAAGCTGTTTTTATTGATTATTTTCAATTGCTTGATAATCAAAAATATTCAAGTGAAAGAGAAAAGTTGTCTCAATGCTCTAAACAATTAAAAGAATTGTCAAAAATATTTAATTGTAATTTTTTTGTTTTATCTCAACTTTCAAGAGAGTATGAAAAGAAAACCAATAATGAACCATCTTTTTCTGATTTAAAAGGAACTGGTTCTATTGAACAAGACGCTGATTTAATTATGTTTCTTTATAATTCTTCAAATAATTTTAATGTTGGCACTTTAATCCCTAACAAGAAAACAATTTTGAACTTATCAATTGCAAAAAATAGAAATGGTGAATTGGTCAAAACAAATATTGATTTTATTCCGCATTTAGCAAAATTTATAGAAAGTTAA", "aa": "MLLTTKQLQEFYKIEMENIAKNKSLLTNFNIIDNYANGLKPKQLYFLAARPGVGKTSFALNIIFNALENLKEDECIVLFSLEMDSIEIYSKLLALANSCSLKEVETNSKKIDFEDLNKKNLFIFDRYRNNFNNEKLSEVTPDIIYKSLNSIGLKIKAVFIDYFQLLDNQKYSSEREKLSQCSKQLKELSKIFNCNFFVLSQLSREYEKKTNNEPSFSDLKGTGSIEQDADLIMFLYNSSNNFNVGTLIPNKKTILNLSIAKNRNGELVKTNIDFIPHLAKFIES", "aa_hexdigest": "4a93e6f8cdf2090e0fd7683161a28ac6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49A9", "ncbi_nrp_id": "WP_013526930.1", "uniref100_id": "UniRef100_C4XDP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49A9", "RefSeq:WP_013526930.1", "UniRef:UniRef100_C4XDP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XDP9", "uniref90_id": "UniRef90_C4XDP9" }, "psc": { "uniref90_id": "UniRef90_C4XDP9", "product": "SF4 helicase domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDP9", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_C4XDP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP9" ], "product": "SF4 helicase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1162", "locus": "GCA000186005_01135" }, { "type": "cds", "sequence": "contig_1", "start": 627003, "stop": 627164, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526932.1", "SO:0001217", "UniParc:UPI00003B49BC", "UniRef:UniRef100_C4XDQ0", "UniRef:UniRef50_C4XDQ0", "UniRef:UniRef90_C4XDQ0" ], "nt": "ATGAATAAAGAAAAACTGATTAAATTTCTTATTGATTTAACAGAAAAAGAAAATACAAAATTAAAATCAGATGTTAAAATTCTAAAATCTAAACTTAAAAAAATAAAAAAAGAATATGAAGAACAAGAAAAAGCTTTTGATGACTTATTAAAAAAACTTTAG", "aa": "MNKEKLIKFLIDLTEKENTKLKSDVKILKSKLKKIKKEYEEQEKAFDDLLKKL", "aa_hexdigest": "b9b9b01faeae9033cd045880907e0c37", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00003B49BC", "ncbi_nrp_id": "WP_013526932.1", "uniref100_id": "UniRef100_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49BC", "RefSeq:WP_013526932.1", "UniRef:UniRef100_C4XDQ0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDQ0", "uniref90_id": "UniRef90_C4XDQ0" }, "psc": { "uniref90_id": "UniRef90_C4XDQ0", "uniref50_id": "UniRef50_C4XDQ0" }, "pscc": { "uniref50_id": "UniRef50_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.590999999999, "isoelectric_point": 9.488601875305175 }, "id": "EFPKPENHBP_1163", "locus": "GCA000186005_01136" }, { "type": "cds", "sequence": "contig_1", "start": 627201, "stop": 627407, "strand": "+", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC", "UniRef:UniRef90_UPI0021806FCC" ], "nt": "TTGTATGATGACGGCAATATGAGTTATTTTAAAGCATTACAAAACAATGAAAATTGTGTTATTTATTCTATAGGAATACAAGAACATAAAGATTTAAAGAATTATTATAATATTGACCTTTCATTAAATAATTTTAATTTAATTGACCAACTAAAACTAATTCCAAAACCTGACATTATAATTGCTTCTCCACCTTGTGAGAGTTGA", "aa": "MYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDLSLNNFNLIDQLKLIPKPDIIIASPPCES", "aa_hexdigest": "3e37fdb238bb2c41f499446157961cd2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 627168, "stop": 627552, "observations": { "insertion": [ 627501 ], "mutation": [ 627195, 627405, 627435, 627459 ], "stop": [ 627195, 627405, 627435, 627459 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021806FCC", "query_cov": 1.0, "subject_cov": 0.5354330708661418, "identity": 0.941, "score": 136.0, "evalue": 4.9e-40, "gene_start": 1, "gene_end": 68, "reference_start": 12, "reference_end": 79, "reference_sequence": "MNENKKITIWALYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDFSLNNVNLINQLKLIPKPDIIIASPPCESWSRADCSLAIWKDISEASWKLNNYEFYKTVETKKTKSEIFTLKNKKEY" }, "paralog": false, "identity": 0.8976377952755905, "score": 223.0, "evalue": 9.25e-77, "description": "Internal stop codon at 627195, 627405, 627435, 627459. Frameshift due to insertion around 627501. Nonsense mutation around 627195, 627405, 627435, 627459.", "psc": { "uniref90_id": "UniRef90_UPI0021806FCC", "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021806FCC", "UniRef:UniRef50_UPI0021806FCC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC" ], "product": "DNA cytosine methyltransferase" } }, "id": "EFPKPENHBP_1164", "locus": "GCA000186005_01137" }, { "type": "cds", "sequence": "contig_1", "start": 627465, "stop": 627611, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAACTATGAATTTTATAAAACAGTAGAAACCACAAAAAACAAAAAAAGAGATTTTTACTCTAAAGAACAAAAAAGAGTATTAGGTGAAAGTACTGCTGGAGCGACGGCTTATATAATTAATGTCTTTCAACCTAAAGTGTGA", "aa": "MNNYEFYKTVETTKNKKRDFYSKEQKRVLGESTAGATAYIINVFQPKV", "aa_hexdigest": "0ddb1aa4599f82ebcc2d737a532efb01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5637.337600000002, "isoelectric_point": 9.573055458068847 }, "id": "EFPKPENHBP_1165", "locus": "GCA000186005_01138" }, { "type": "cds", "sequence": "contig_1", "start": 627669, "stop": 627968, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526934.1", "SO:0001217", "UniParc:UPI0001F5AD4A", "UniRef:UniRef100_A0AB32XBS1", "UniRef:UniRef50_A0AB32XBS1", "UniRef:UniRef90_A0AB32XBS1" ], "nt": "TTGAATGGAATTAAAAATAATACCTATTACTCAAGCTATAACAAAGAATTTAGTGTTAAGCCAACTTGTTTTTATTCAAATATTAATTTAAATTTGAAAAACAAAAGAAAACCAGGCAATGCTGAACACTATTCAAAAGGTAGTTATTATCAACGCTCAATGATTCCAAAAGAATTAATTAAAGATTTATTTTTAAATGTTTTTGTCCAATTGAAAAAAATTTCTGTATTAAGTAAAGAATATACAAAAAACTATATTTCAAATTCACTAGAGAAAGATCAGATCTCCTTATTTGATTAA", "aa": "MNGIKNNTYYSSYNKEFSVKPTCFYSNINLNLKNKRKPGNAEHYSKGSYYQRSMIPKELIKDLFLNVFVQLKKISVLSKEYTKNYISNSLEKDQISLFD", "aa_hexdigest": "156bf1aca29644cfe6b9ec01445bd65c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5AD4A", "ncbi_nrp_id": "WP_013526934.1", "uniref100_id": "UniRef100_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD4A", "RefSeq:WP_013526934.1", "UniRef:UniRef100_A0AB32XBS1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBS1", "uniref90_id": "UniRef90_A0AB32XBS1" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBS1", "uniref50_id": "UniRef50_A0AB32XBS1" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBS1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11665.21419999999, "isoelectric_point": 9.567897987365722 }, "id": "EFPKPENHBP_1166", "locus": "GCA000186005_01139" }, { "type": "cds", "sequence": "contig_1", "start": 628402, "stop": 628746, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCTAAAAGAACCTCCTGTTTTTACAGAAGAACAATTTAAAGAATTAGTTCATTATACTGATTTAGAAGATAATCCAACTTTTGAAGCTTACAAATCTAATGCATTTTTCAAAATCTATTCAGATGCTAAAGGTGTTTCAAAATCTTTTAGTAGAATGATTGAAACTGTTTATAGATTAGTGAATGAAAAAAATTTTAATAGTTTTTGGTGTAGAAATCAATATAACCACATTAAAGGAACATTAAAACCATTATTAGAAAAAGTTTTAAGTTTTCTTGCTGCTAAAGGTTTAGATTATAGACCTTATATTAGTATTTACAATACTGAAGCTTATTGA", "aa": "MKFLKEPPVFTEEQFKELVHYTDLEDNPTFEAYKSNAFFKIYSDAKGVSKSFSRMIETVYRLVNEKNFNSFWCRNQYNHIKGTLKPLLEKVLSFLAAKGLDYRPYISIYNTEAY", "aa_hexdigest": "520962d1ecdaad8930985ed86dc5849a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13546.303799999983, "isoelectric_point": 8.573408699035646 }, "id": "EFPKPENHBP_1167", "locus": "GCA000186005_01140" }, { "type": "cds", "sequence": "contig_1", "start": 629065, "stop": 629280, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAGGAACAGATTTTTTAAAGAAGATCAATTTAAATTTAGCAAAAGAATTAATTGAAAATACTTATTTGCAAAAAGAAGACTTAGCTTTTAAAGAGGGAATGGGAATTATTGTCACAATGTTTTCTAAATATTTTGTTCCTAAAAATGAATTAAGTGATTTGCAATTAAAACAATTTGAAGCTCTAAAAACTAAAGATTATAAATTATGA", "aa": "MYSGTDFLKKINLNLAKELIENTYLQKEDLAFKEGMGIIVTMFSKYFVPKNELSDLQLKQFEALKTKDYKL", "aa_hexdigest": "b97bc9f1af0001dfc0b4eb58d428ffb2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8328.668299999996, "isoelectric_point": 7.8927515029907225 }, "id": "EFPKPENHBP_1168", "locus": "GCA000186005_01141" }, { "type": "cds", "sequence": "contig_1", "start": 629281, "stop": 629373, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTGTTGCTGGTTTTGCTTTTGAAGAGCCACCTGAAAGATATGATTATTTTATGAAAAGTGTTTTGCTCAACAAAAATGGCAATTGA", "aa": "MITVAGFAFEEPPERYDYFMKSVLLNKNGN", "aa_hexdigest": "80388edbdc6eb996ef0a3dcc1fa895a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3481.947400000001, "isoelectric_point": 4.867827796936035 }, "id": "EFPKPENHBP_1169", "locus": "GCA000186005_01142" }, { "type": "cds", "sequence": "contig_1", "start": 629731, "stop": 629832, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAGTTTTTATTAACAATGGGATAAATACTAAATTGTTACCTGCTTCAAGATTTAAAGGCAAAAAAGGCTTTGGAATAATTGACAGACAAGTATGA", "aa": "MTEVFINNGINTKLLPASRFKGKKGFGIIDRQV", "aa_hexdigest": "c94b5dbbcc53bf80dbeedb9fc5400956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3693.3227000000015, "isoelectric_point": 10.446408653259276 }, "id": "EFPKPENHBP_1170", "locus": "GCA000186005_01143" }, { "type": "cds", "sequence": "contig_1", "start": 630065, "stop": 630196, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAACAATTATTAATAAATTAGAAAATGAAAGCAACAAATATTTTAGTTTAAGGTTTAATAATAAACCTGATTCATATAATCAATTTTTAAAATTTGGAACTCAAACAACTATTAACACTATTTGA", "aa": "MSKTIINKLENESNKYFSLRFNNKPDSYNQFLKFGTQTTINTI", "aa_hexdigest": "642a23fba149adc6f0842031d0c11584", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.690500000001, "isoelectric_point": 9.601614952087402 }, "id": "EFPKPENHBP_1171", "locus": "GCA000186005_01144" }, { "type": "cds", "sequence": "contig_1", "start": 630329, "stop": 630442, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCAAGTTTTAGATTATTTGCTTGATAGAAATGGTAACTTGGTAAAACTTAAAACAAGAATCAAATTTTATTCATCAAATATAAGTTTCTATATTGATGAAGAGTGA", "aa": "MPAQVLDYLLDRNGNLVKLKTRIKFYSSNISFYIDEE", "aa_hexdigest": "c5530ee6537ad677a5cf30013a1d86db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4394.997, "isoelectric_point": 6.101006507873534 }, "id": "EFPKPENHBP_1172", "locus": "GCA000186005_01145" }, { "type": "cds", "sequence": "contig_1", "start": 630734, "stop": 631129, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTCTTAAAGACATGATCAAACAAGGACTTAAAGATCCTAATGATAGATTTATTCCAATTAATACTGCTATTCAAATGGATGGAACTCCTCCTGTTCAACTTGTGCAAGGCAATAGTCAAGCAATACCATTAATTGAAAAAATAAGATTCAATATTAAACAAATTAAAAAATTTGCTTTCTTAAAAGAAGATTCAAGTGATTCTGGAACTAAAAATATGCATAATGCTGAAGTTCAACAAATTAATAGTGATTTTGAAGATCATATTGAAGCAAAATCAAACTTAAGACAAATGCAACTATTAAACTTTTTCAAAACTTTTGAACCTAGTTTGCTAATTGATAAAGTTTTAATTTTTGGTTCAACTGAATGGCTAAAAAATGAAGCTTGA", "aa": "MNGLKDMIKQGLKDPNDRFIPINTAIQMDGTPPVQLVQGNSQAIPLIEKIRFNIKQIKKFAFLKEDSSDSGTKNMHNAEVQQINSDFEDHIEAKSNLRQMQLLNFFKTFEPSLLIDKVLIFGSTEWLKNEA", "aa_hexdigest": "0dd1a897a9693c93b3eab5c088ec459b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14978.032499999988, "isoelectric_point": 6.10345058441162 }, "id": "EFPKPENHBP_1173", "locus": "GCA000186005_01146" }, { "type": "cds", "sequence": "contig_1", "start": 631224, "stop": 631334, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTTATGGAATTAAAATCTTTTTTAGAGCCAAGAACTGGTGAATATATTTTAATAGACAATAAATCACTTTCTTTTGAAAATGGTTCAGTTAATGTAATATCTTGA", "aa": "MDFMELKSFLEPRTGEYILIDNKSLSFENGSVNVIS", "aa_hexdigest": "53b58b1c26164d20f808658d99ee974a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.644200000002, "isoelectric_point": 4.3588346481323255 }, "id": "EFPKPENHBP_1174", "locus": "GCA000186005_01147" }, { "type": "cds", "sequence": "contig_1", "start": 631479, "stop": 631871, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACTAGATAATAAATTTTATATTTTAGATGCTTTAAATAACCAATATGATTTGGAGCAAAAAAATAATGCTTATTCATATGGAATAGCTCAACCAATTTCTCAAATTGAAAATGAGAAAAAAATAAAATTTGTTTTCATTGAAGAAAAGCCAAATGAAATTAATGTCAGTTTAAGAACTCCATATTTGCACCAAAAAGCTTTAGAAATTGAAAACAATGGTTTAACTAGTGAATTGCCAACAAATGGCCTAGCTTTTGATATGTTTGTTAATCCAATTGAAATTAATGGTATTGACAATAAATACTATTTAAATACTTTGAGCAAATTTTCTCATTTATATTTGAATATTATTTTGAACAAAGAAAATGATTTAATGAAAAACTTTTGA", "aa": "MKLDNKFYILDALNNQYDLEQKNNAYSYGIAQPISQIENEKKIKFVFIEEKPNEINVSLRTPYLHQKALEIENNGLTSELPTNGLAFDMFVNPIEINGIDNKYYLNTLSKFSHLYLNIILNKENDLMKNF", "aa_hexdigest": "050fd94a5c6fe42199a7b8547b95ee1b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15210.12469999998, "isoelectric_point": 5.154751014709471 }, "id": "EFPKPENHBP_1175", "locus": "GCA000186005_01148" }, { "type": "cds", "sequence": "contig_1", "start": 631923, "stop": 632162, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATGACTGAAGAAGAAATAAAAAAATTTAAAGAGACTATTTTTGCTGGAAACAATTTAAAAGATTTTGTTGATTTTAAATTTTACACTTTGATTAACTTATATACAAAGAAAGAAAAATTTTTAAAAACTTTATTTGCAATGAGTTCAGGCAATGTTCCTTCAACATATTGTATTGATAATGGTGAAACTGAAAAACATGAACTTTATTTGCCTTTTTATTTTGATAAAAATTGA", "aa": "MGMTEEEIKKFKETIFAGNNLKDFVDFKFYTLINLYTKKEKFLKTLFAMSSGNVPSTYCIDNGETEKHELYLPFYFDKN", "aa_hexdigest": "ca3d6f91fed08138f14434c30ee4989d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9356.634399999995, "isoelectric_point": 5.774637031555175 }, "id": "EFPKPENHBP_1176", "locus": "GCA000186005_01149" }, { "type": "cds", "sequence": "contig_1", "start": 632355, "stop": 632900, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTGACCAAAAACATGAAGAATTTTTAATGTTTAATAAAGAAAAATTTAATGAAGCTTTTCAATTATCATCAATTCAAACTTCTAGATATACTATGACACCAGAAGAAGCTAGAAAAGAAATTAAAGACTTAGAACCTGGTTTAACTAAAAAGCAAATGTTTGTTCAATTAATTAAAAAAAGCGTTAAAAAAGAAATAAACATTGTTAACGCTTTTGCAAAGGTTCAATTAAACCCAACATATACAGCTAAAATTGCACATGGTGATGAACCTGGAATGATTCATTATACTTATGACACAATTCCTGCTGCATTTAAAATTGTTGATTTAGATGATTTAAATGATTGGTTAGAAGTTGTTAATTTTTCAGGTCTTGATTACAAAAATCTTGACGATAACTATGAATTAATAAATGAAACTTATGTTCCTATTTATACAATTGAATATCAAGTATATGAAAAAGAAGATTTTAAATCAAATATATATGACACTTATTTTAAGTTTCTAAACAATGATTTTATTATCAATAAAAATCAATGA", "aa": "MFIDQKHEEFLMFNKEKFNEAFQLSSIQTSRYTMTPEEARKEIKDLEPGLTKKQMFVQLIKKSVKKEINIVNAFAKVQLNPTYTAKIAHGDEPGMIHYTYDTIPAAFKIVDLDDLNDWLEVVNFSGLDYKNLDDNYELINETYVPIYTIEYQVYEKEDFKSNIYDTYFKFLNNDFIINKNQ", "aa_hexdigest": "e48a24573744bd0463c5248f7d8d07ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21492.101900000005, "isoelectric_point": 4.87601261138916 }, "id": "EFPKPENHBP_1177", "locus": "GCA000186005_01150" }, { "type": "cds", "sequence": "contig_1", "start": 633113, "stop": 633436, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6", "UniRef:UniRef90_C4XDQ6" ], "nt": "ATGCATAAAATTATTAGTGTTTTATCTAGCAAACTAGGAATTGAAGAAAGTGAACTCCTTAAAAAACTTTCATTAAATGAAAATTCTTCTCAAAAGGAGATTGCTTCTTCGCTTGGAGTTTATGAGTTTTTCCAAGAAAAAGATGACTTGACTTCATTCATCTTAAATAAAACTCAAAACCAACATAAAGATAATCAAAATTTAAAAACTGAAAATGAAAAGTTAAAAAATGATTATCAAAGTCTTGAGCAACAATTCAACACCAATTTAGAAAATTATAAAAACTTGTCCGGAAAAGTTAAAGATACTTTTAAAAATGAATGA", "aa": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNE", "aa_hexdigest": "2df2c8230f62cbfa481e4dfd77cc15a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 633113, "stop": 633643, "observations": { "mutation": [ 633434, 633617 ], "stop": [ 633434, 633617 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDQ6", "query_cov": 1.0, "subject_cov": 0.6079545454545454, "identity": 0.981, "score": 173.0, "evalue": 1.93e-53, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNEWNKYKLNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSINNINQDSSSNSSDPWSPYIRNY" }, "paralog": false, "identity": 0.9772727272727273, "score": 315.0, "evalue": 6.92e-112, "description": "Internal stop codon at 633434, 633617. Nonsense mutation around 633434, 633617.", "psc": { "uniref90_id": "UniRef90_C4XDQ6", "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDQ6", "UniRef:UniRef50_C4XDQ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12444.701699999987, "isoelectric_point": 5.738146400451661 }, "id": "EFPKPENHBP_1178", "locus": "GCA000186005_01151" }, { "type": "cds", "sequence": "contig_1", "start": 633449, "stop": 633619, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAATTGATTTTGAGAAGATAGATGTAAATTCAATTAATCTAAATAATTTGTTTCAAAGTGTTGAGCAAGTGGCAAAAGAAAATAACATAACTATTAAGACTAAAGAACCTAATTCTGTAAATAATATTAACCAAGATTCTAGTTCTAATAGTTCTGATCCATGA", "aa": "MNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSVNNINQDSSSNSSDP", "aa_hexdigest": "478824230b7a48d6d4a4cf1a37b17b6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6281.705800000002, "isoelectric_point": 4.207586097717285 }, "id": "EFPKPENHBP_1179", "locus": "GCA000186005_01152" }, { "type": "cds", "sequence": "contig_1", "start": 633656, "stop": 633883, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATCAAATACCAACTTTAAAACAATATTTAACTGCTGAATCTGCTACTTTATTTGAACAAGGAGCTGATAAAGCTTATACCATATCAAATGGTGGTCTAACTAAAATTTCAACTTTATTGAATGCAATTTTAGAAACAAACTTTTTAGGTAGTACATTAACTCCTGCAAGTGATCCAAATTCACCAGTTATTGAATATTTAATTTCTTTAGATCAAGAATGA", "aa": "MSYQIPTLKQYLTAESATLFEQGADKAYTISNGGLTKISTLLNAILETNFLGSTLTPASDPNSPVIEYLISLDQE", "aa_hexdigest": "8f6d975d7cc68fc30c5c7462443b62c8", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8113.025099999999, "isoelectric_point": 4.246691322326661 }, "id": "EFPKPENHBP_1180", "locus": "GCA000186005_01153" }, { "type": "cds", "sequence": "contig_1", "start": 634166, "stop": 634387, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAAAAAAACAGATGAAGAAGTTTATAGTGAAATTGTAAACATGGCATTAAGATTAATGGAACATGAAGATTCAACATTAGGAATTGACGGAATTGAACAAAGCAAAATTGGAATTTTTGTCAAACCAAGTATTTATCAAAAAATAGTCAGATATTCAACACAAGGAAACTTTGTAAAAGAATCAATTCAAGGTAATCCTAAAGACCCAAGAACATGA", "aa": "MTKKTDEEVYSEIVNMALRLMEHEDSTLGIDGIEQSKIGIFVKPSIYQKIVRYSTQGNFVKESIQGNPKDPRT", "aa_hexdigest": "bc0ef6f55afd932b9a9a0218ba8d1c7a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8333.397099999996, "isoelectric_point": 5.746331214904786 }, "id": "EFPKPENHBP_1181", "locus": "GCA000186005_01154" }, { "type": "cds", "sequence": "contig_1", "start": 634553, "stop": 634648, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCAAAACTTTTTTGGAATAGTTTGTGATTCAAACTTTGAAGCTATTAAAAAACCAACAACTGCACCAAGTCCAAAACCTGGCTCTTAA", "aa": "MEIQNFFGIVCDSNFEAIKKPTTAPSPKPGS", "aa_hexdigest": "219b1dd5e5d2ebd9958b8645f900d938", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3354.805000000001, "isoelectric_point": 5.934922981262207 }, "id": "EFPKPENHBP_1182", "locus": "GCA000186005_01155" }, { "type": "cds", "sequence": "contig_1", "start": 634651, "stop": 634917, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATTCTAACCCAAAACCAAAAACAACCTTGCAATTAAGCCCAAAATCATTTGTTATTAATGATTCAAAAAAACAAATGAATCCTTTTTTGCGAGGTTTTCTTTCATTCCTACATGTTGCTTTGGATATAGGAGCAAGTTTTTTAGGTAGTTTTATTAATACCTTTGCGCCTGGTCTTGGTCTTGTTATTGAATATGGTCTAAGTACTGCCTTAGATTTAGGTTTTAATGCTGCTATAAACTATGGAAATATCAATTGA", "aa": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFINTFAPGLGLVIEYGLSTALDLGFNAAINYGNIN", "aa_hexdigest": "babb1ec3af0953af48148397bc63a708", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9554.99199999999, "isoelectric_point": 9.157943534851075 }, "id": "EFPKPENHBP_1183", "locus": "GCA000186005_01156" }, { "type": "cds", "sequence": "contig_1", "start": 634960, "stop": 635442, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B", "UniRef:UniRef90_UPI000FF6197B" ], "nt": "GTGACAAGAGGAATAAGATATGCAGCAAACACACATAAAACTAACAAGTTTTTTCAAATTGCAAAAAATCTTAAAAAAGAGGGTCAAGAAGAATTAAGTGAAAAAATAACAAAAATAGCTGCTAATTATTCTGAATTAGGAGTTGACAAAGCTAAATTTATTGACAATAAATCACTTTTAAAAAAAGAACTAAAATTATTTAATAATGATTCATTTTTGAGAACTTTATCTGATAACAAAAATATTTATTATTTTTTGAATAAAAAGGTTCAAAATATACAAAAAGCTTTCACACATTTAAGATCTATGGTCTCATTATTAACCTCTCCCTCATATTTAGCAAAAAAAGCAGTTGACACTGTATTTAAACACCCAAAAGCCTATTTGAACAAACAACTTGGCAAATTTACCAAATTATTAAAAAATACTTTTAAATCAAAACCAATTAAGAAAGTAGTTAAGAAAGCTTCTAATTATATTTGA", "aa": "MTRGIRYAANTHKTNKFFQIAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDNKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVDTVFKHPKAYLNKQLGKFTKLLKNTFKSKPIKKVVKKASNYI", "aa_hexdigest": "f2b10e432bf332c6d2062da8dfad3aa6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 634660, "stop": 635380, "observations": { "insertion": [ 635314 ], "mutation": [ 634915 ], "stop": [ 634915, 635345 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF6197B", "query_cov": 0.8875, "subject_cov": 0.5867768595041323, "identity": 0.8590000000000001, "score": 218.0, "evalue": 3.07e-69, "gene_start": 1, "gene_end": 142, "reference_start": 104, "reference_end": 238, "reference_sequence": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFVNTFAPGLGLVIEYGLSTALDLGFNAAINYGNINWQDFGISAGINAIPFVTRGIRYAANTHKTNKFFQLAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDQKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVALYLNTQKPIWTNNLANLQNY" }, "paralog": false, "identity": 0.9707112970711297, "score": 451.0, "evalue": 2.14e-163, "description": "Internal stop codon at 634915, 635345. Frameshift due to insertion around 635314. Nonsense mutation around 634915.", "psc": { "uniref90_id": "UniRef90_UPI000FF6197B", "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF6197B", "UniRef:UniRef50_UPI000FF6197B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18511.5323, "isoelectric_point": 10.1827974319458 }, "id": "EFPKPENHBP_1184", "locus": "GCA000186005_01157" }, { "type": "cds", "sequence": "contig_1", "start": 635611, "stop": 635817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1", "UniRef:UniRef90_UPI000FEDB9C1" ], "nt": "TTGATTAAATTAGTTTGTGCTAAAAGTCCTGGAAGATACTATTTAAATAATATTGCTTATGGCCATATAGTAGGAAGATTTATTAGAAAATTTGAAATGTTTCAAGCTGTTCCAATACACCCTATTTTCTCCAATTTAATAGGAACTTCATTATTTACTTTTAGAACAATTAATTCTCTTATTATGAATTTTAAAAGACAAAAATGA", "aa": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQK", "aa_hexdigest": "bd01a20bf3fc41619262206686a58e9c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 635611, "stop": 636066, "observations": { "mutation": [ 635815, 635821, 635890, 635935, 635980, 636028, 636040 ], "stop": [ 635815, 635821, 635890, 635935, 635980, 636028, 636040 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "query_cov": 1.0, "subject_cov": 0.4503311258278146, "identity": 1.0, "score": 141.0, "evalue": 1.06e-41, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQKWVWLEDKNIILNEIVKGVKENVFKGWRAPGFQFISSMARSWATGNVGYQTKAALRWANKKAFTSKTQKTNYWVNHWVKNKYKK" }, "paralog": false, "identity": 0.9470198675496688, "score": 276.0, "evalue": 1.58e-97, "description": "Internal stop codon at 635815, 635821, 635890, 635935, 635980, 636028, 636040. Nonsense mutation around 635815, 635821, 635890, 635935, 635980, 636028, 636040.", "psc": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEDB9C1", "UniRef:UniRef50_UPI000FEDB9C1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7952.441199999995, "isoelectric_point": 10.73645191192627 }, "id": "EFPKPENHBP_1185", "locus": "GCA000186005_01158" }, { "type": "cds", "sequence": "contig_1", "start": 636266, "stop": 636523, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTTGTTAAAACTCAAAGTGACTCCAAAGCTATTAAAAATATTATTGGAGAAACAAGATTTGAAAACTTCAAAAAAGCTATTTATTTGGAAATTAAATATAGACTTTCAAGTAAAAGTTTTCCAGAATATTCAAATAGTGTTTTTCTGGTTTCTAATAATATTTCACTTAATGGTGAAACAAGAGATTTTGGTTCATTATCAAAATTAATAAATTATGAATCTATTCAATTATTAACATTTCCAAGTTGA", "aa": "MSYFVKTQSDSKAIKNIIGETRFENFKKAIYLEIKYRLSSKSFPEYSNSVFLVSNNISLNGETRDFGSLSKLINYESIQLLTFPS", "aa_hexdigest": "ae7805db04171dbe811287e695ce2f8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9788.01729999999, "isoelectric_point": 9.259674644470213 }, "id": "EFPKPENHBP_1186", "locus": "GCA000186005_01159" }, { "type": "cds", "sequence": "contig_1", "start": 636575, "stop": 636778, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCATTAAAAGAATTTGTTAACAATAAAGATATTGAATTAGAAAACAAAATAAAAAGCTTGGAAGAATATGACAAATCTCAAACTCAAAATAATATTAATCAAGAAGCGCGTATTAAAAATTTAGAATATAAAACTAATGTTATTAAAAGCAAAAGCGAGATACGACTTGAAGACAATGAGGGAAAATTTCATTTATGA", "aa": "MKSLKEFVNNKDIELENKIKSLEEYDKSQTQNNINQEARIKNLEYKTNVIKSKSEIRLEDNEGKFHL", "aa_hexdigest": "b8d6947ecc42db5bd9d1dded588e4115", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7998.901199999998, "isoelectric_point": 6.583569526672363 }, "id": "EFPKPENHBP_1187", "locus": "GCA000186005_01160" }, { "type": "cds", "sequence": "contig_1", "start": 636812, "stop": 637021, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTATGCATTTCAAAATGAAAGTTTTAAAAAACTAAAAGACATTTTAGACAACTACGAAAATTTAAAGCAAGCTTTTGAATCCTTTAAAATTGCGCAAGAAAACCAAAATACTAAAAATGTCCAAATCAATTCCACACAAGATAATCAAATAAAAGCTTTAGAAGATAAAGTTGCTACTTTAGAAAATACCACATCTAATGTTTGA", "aa": "MLYAFQNESFKKLKDILDNYENLKQAFESFKIAQENQNTKNVQINSTQDNQIKALEDKVATLENTTSNV", "aa_hexdigest": "60efe6ccc3bc9e3f07fae9f50c636036", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8000.782500000001, "isoelectric_point": 4.927224540710449 }, "id": "EFPKPENHBP_1188", "locus": "GCA000186005_01161" }, { "type": "cds", "sequence": "contig_1", "start": 637652, "stop": 637762, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4", "UniRef:UniRef50_A0AB32XCS4", "UniRef:UniRef90_A0AB32XCS4" ], "nt": "ATGAAAATTGAAAAAAGAAATTTAGCTAAAAGAATCAAAGAAGCTTCCAAAGCTTTGAAAACAAATACATTAGACAATAAAATTGAACATGATAAGAAAGGAAACAAATAA", "aa": "MKIEKRNLAKRIKEASKALKTNTLDNKIEHDKKGNK", "aa_hexdigest": "b832ac12d69be293f91c2f306a418c97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59902", "uniref100_id": "UniRef100_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCS4", "uniref90_id": "UniRef90_A0AB32XCS4" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCS4", "uniref50_id": "UniRef50_A0AB32XCS4" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.8879000000015, "isoelectric_point": 10.170935249328611 }, "id": "EFPKPENHBP_1189", "locus": "GCA000186005_01162" }, { "type": "cds", "sequence": "contig_1", "start": 637762, "stop": 638409, "strand": "+", "frame": 1, "gene": null, "product": "Mem", "db_xrefs": [ "RefSeq:WP_013526945.1", "SO:0001217", "UniParc:UPI00003B49C6", "UniRef:UniRef100_C4XDR0", "UniRef:UniRef50_Q5QGJ3", "UniRef:UniRef90_Q5QGJ3" ], "nt": "ATGAAAGATTTTTTCGCAAACCCAACTAACTTAATTATATTTTTAGGTGGTTTTGTTTCTTTAATAGCTTCTATTACTGGAATCACAACCACTTTCATTAAAACTCATAATTTAAAGCTTCAAAATAATAACCTTAAACAAGAAAATAAAGACATAAGATTTGAACTTCTTCAAACTCAAAAAGAATTGGCTGATAAAAACAATTCACTTTCAAAACTTGAAGTTATGTTTGACAACCAAAGAAAAGAAATTGAAAGACTAAGTGAAGAATTAAAAGCTTTACAAAGTGGCAATGTTGATCCTTTGGTTAAAAAGCTAAAAGAACAAATTGAGAACCTTGTTAATGAAAAGAAACAATTAACTGAACAAGTCTATAATGTTAAAGCTGAATTAAATGAGTCTAAATATCATTCTGGAGCTTATGCAAATGAAATTGTTGAATTGAAAAGGGAAAATAGTGAGCTTAAAGAGCAATTAAAGCTTGAAAGAGCTAAGATTGTTGAAGACATAGCAAATATATTAACTCCCAAAAACAATGAGGCAAATAAGGCTTCTAATGAAGCAATAAAGGAAAATAAAATAGAAGCCCCAAAAGCAGAAACCTTTGAAGCTCCAAAAAATAATGAAAATGTTAGCCTATATATTTAA", "aa": "MKDFFANPTNLIIFLGGFVSLIASITGITTTFIKTHNLKLQNNNLKQENKDIRFELLQTQKELADKNNSLSKLEVMFDNQRKEIERLSEELKALQSGNVDPLVKKLKEQIENLVNEKKQLTEQVYNVKAELNESKYHSGAYANEIVELKRENSELKEQLKLERAKIVEDIANILTPKNNEANKASNEAIKENKIEAPKAETFEAPKNNENVSLYI", "aa_hexdigest": "e970ed109eecf36c29fe7895627855cd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49C6", "ncbi_nrp_id": "WP_013526945.1", "uniref100_id": "UniRef100_C4XDR0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49C6", "RefSeq:WP_013526945.1", "UniRef:UniRef100_C4XDR0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDR0", "uniref90_id": "UniRef90_Q5QGJ3" }, "psc": { "uniref90_id": "UniRef90_Q5QGJ3", "product": "Mem", "uniref50_id": "UniRef50_Q5QGJ3" }, "pscc": { "uniref50_id": "UniRef50_Q5QGJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5QGJ3" ], "product": "Mem" }, "genes": [], "id": "EFPKPENHBP_1190", "locus": "GCA000186005_01163" }, { "type": "cds", "sequence": "contig_1", "start": 638469, "stop": 638843, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGACACTTCTTTATTGTTAATGCTCATTTAAACATTGGAAGAACAGAAGAAGTTGCAAAGCTTGTTGGTCATGTTAACTCACAAACAACAGCAACAACATATTTAAACTTTAGCAATGTTGCTCTTGAAAATATTATGGAACAAGCTCAAATGGAAAATGAAAGTGCTTTTGACTATTCAAAAATTAAAATTAAATATAAAGAACTAAGCGAAGAAAATGCTCAACTCAAATTAACAATTAGTGAACTAAACAACCAATTAGACAAATACAAAAAAGTTTTAGGTGAAACAAAAGAAAATGTTAATTTTACAGAAGTATTAAATAGCAATATAAGTAAATTAAAAAATTTAATTAAAAATATTAAGTAA", "aa": "MLRHFFIVNAHLNIGRTEEVAKLVGHVNSQTTATTYLNFSNVALENIMEQAQMENESAFDYSKIKIKYKELSEENAQLKLTISELNNQLDKYKKVLGETKENVNFTEVLNSNISKLKNLIKNIK", "aa_hexdigest": "556fdf48c55ed646aa196dd5a0660b85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14263.132599999979, "isoelectric_point": 7.916024589538574 }, "id": "EFPKPENHBP_1191", "locus": "GCA000186005_01164" }, { "type": "cds", "sequence": "contig_1", "start": 639090, "stop": 639212, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAATATTTAAAAATATTCAATTATGTTGAATTATGGGGAGATCTTAAATTCAAATTAGTATTCCAAATTTTAGCTATTACAGGAGTTAGAATTGGAGAACTTGCAAATGTTAATTGA", "aa": "MEEYLKIFNYVELWGDLKFKLVFQILAITGVRIGELANVN", "aa_hexdigest": "c802affec5a2eff5b9b4a496be02debb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4688.4856, "isoelectric_point": 5.002479362487793 }, "id": "EFPKPENHBP_1192", "locus": "GCA000186005_01165" }, { "type": "cds", "sequence": "contig_1", "start": 639354, "stop": 639560, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTACAAAAATAAAATTGTTTTAAATAAAGAAATTGACTATGTTAAAGTTTACTTAGATCAATTTAAAATCTTAGTTGAAAGAAAACAAAAAAGTAAAAAAACTTTTCAACATTATTTAACTTTATTGAAAAGACTTAAAAGATTTGGATCAGTTGAAGAATTACTAGACCAAATGAATCATTATTTAGCTTCTTCAAATTGA", "aa": "MFYKNKIVLNKEIDYVKVYLDQFKILVERKQKSKKTFQHYLTLLKRLKRFGSVEELLDQMNHYLASSN", "aa_hexdigest": "14ae6d86cf0451ed63e4c3bddb6dc20a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8313.694899999997, "isoelectric_point": 9.776324272155762 }, "id": "EFPKPENHBP_1193", "locus": "GCA000186005_01166" }, { "type": "cds", "sequence": "contig_1", "start": 639629, "stop": 639778, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6", "UniRef:UniRef90_UPI001004FEA6" ], "nt": "ATGTTTGAAAAAATAAAAATGAACAGATGGCGTTGCCGTTGTTGTCAAATTTTGACAAATGACAAAAAGAGAGAAGTTTTTAATTATAAAAATGAAGAATATGTGTTTCCTTATTTTTGTCAAAAATGTTGTCAAAGATTAATTAATTGA", "aa": "MFEKIKMNRWRCRCCQILTNDKKREVFNYKNEEYVFPYFCQKCCQRLIN", "aa_hexdigest": "dbe65f769164264e21a35b9da30dfa0b", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001004FEA6", "query_cov": 1.0, "subject_cov": 0.8909090909090909, "identity": 1.0, "score": 114.0, "evalue": 1.35e-32, "valid": true, "uniref50_id": "UniRef50_UPI001004FEA6" }, "pscc": { "uniref50_id": "UniRef50_UPI001004FEA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6262.3648, "isoelectric_point": 9.21532039642334 }, "id": "EFPKPENHBP_1194", "locus": "GCA000186005_01167" }, { "type": "cds", "sequence": "contig_1", "start": 640427, "stop": 640522, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAGTGAAAAAGAATATTTTGAAAGAAATGGTATGAAATTTCTAAAACAAACAGAACAAAGAATAGATATTGTTGAAGAAAGATTATGA", "aa": "MLISEKEYFERNGMKFLKQTEQRIDIVEERL", "aa_hexdigest": "29fdb8ea267d583168c0458b7e0f4927", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3874.4435999999996, "isoelectric_point": 5.189195442199705 }, "id": "EFPKPENHBP_1195", "locus": "GCA000186005_01168" }, { "type": "cds", "sequence": "contig_1", "start": 641036, "stop": 641236, "strand": "+", "frame": 2, "gene": null, "product": "Archaeal holliday junction resolvase (hjc)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36", "UniRef:UniRef90_C4XF36" ], "nt": "TTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGA", "aa": "MSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIA", "aa_hexdigest": "0f7b71e56878e69c8b68c51f46a7ce5f", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 640775, "stop": 641272, "observations": { "mutation": [ 640889, 641234, 641252 ], "stop": [ 640889, 641234, 641252 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF36", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.985, "score": 125.0, "evalue": 1.78e-35, "gene_start": 1, "gene_end": 66, "reference_start": 88, "reference_end": 153, "reference_sequence": "MYENFSRYNLTMKMPNYKDLNFNIKRELNKLKPKSFEEWKEAKSKVFCLALGKYLTKINSHQPDIFKNYYQVLFYHSFINYKREEELLSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIAWKESTEWKFKEI" }, "paralog": false, "identity": 0.9757575757575757, "score": 319.0, "evalue": 3.09e-114, "description": "Internal stop codon at 640889, 641234, 641252. Nonsense mutation around 640889, 641234, 641252.", "psc": { "uniref90_id": "UniRef90_C4XF36", "product": "Archaeal holliday junction resolvase (hjc)", "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF36", "UniRef:UniRef50_C4XF36" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36" ] } }, "id": "EFPKPENHBP_1196", "locus": "GCA000186005_01169" }, { "type": "cds", "sequence": "contig_1", "start": 641259, "stop": 641837, "strand": "-", "frame": 2, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDR5", "UniRef:UniRef90_C4XDR5" ], "nt": "GTGTCAAAGGAAAATGGTATAAGAATATTAAGAATATCTGATTTTGATGAACGTGGTTTAAAAAATAACAATATTGTTTATTATAAATATGAAAGTAAAATGTTTGATTATTTTTTAAATAATAAAAACATTGTTATTTGTATGACAGGTGGAACAGTTGGTAAATCTTTATTAATTAAAGAATTAAAAGAAAAAATTCTTGTAAATCAAAGAGTTGGCAATATAAAAATATTAAATGAAATGTTACCTGATTATGTAGATATTGTTATGAAAAGTGAACTAATATCAAAAATTATCAGAAAAAATAAAAATTCTACAAATGATAATATTTCAATAGAGCTCATAAAATTGTTTTTTATTCCAGTTCCCTCTATTGAAGAACAATTAAAAATTATAGTTAAATATAACAAATTGTTAACACAATTGACATTATATAAAAATATATTCCTTTATATATTCCCACTATATATTCCCGTCAATATCTGGTATTTAAGATTTTTAGTGAATAAAACAATTCACTTTTCAACTTTATTTTATATCAAAATTTTAAATATGTTAAAAAATGTTAAATTTCTTTGA", "aa": "MSKENGIRILRISDFDERGLKNNNIVYYKYESKMFDYFLNNKNIVICMTGGTVGKSLLIKELKEKILVNQRVGNIKILNEMLPDYVDIVMKSELISKIIRKNKNSTNDNISIELIKLFFIPVPSIEEQLKIIVKYNKLLTQLTLYKNIFLYIFPLYIPVNIWYLRFLVNKTIHFSTLFYIKILNMLKNVKFL", "aa_hexdigest": "531f73d07ba47cb7c445658d07b232a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 641208, "stop": 642137, "observations": { "mutation": [ 641261, 641900, 641906 ], "stop": [ 641261, 641900, 641906 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDR5", "query_cov": 1.0, "subject_cov": 0.5907692307692308, "identity": 0.995, "score": 362.0, "evalue": 3.54e-124, "gene_start": 1, "gene_end": 192, "reference_start": 117, "reference_end": 308, "reference_sequence": "MKINRLTAEQLRKSILQLAIQGKLVKQDPNDEPASKLIERIYQEKQKLIKEGKIKKDKQESFIFKGEDNHYYEKIGKNIKDITEELPFEIPENWMWVRLKNISIINGGFAFKSSEFVSKENGIRILRISDFDERGLKNNNIVYYKYESKMFDYFLNNKNIVICMTGGTVGKSLLIKELKEKILVNQRVGNIKILNEMLPDYVDIVMKSELISKIIRKNKNSTNDNISIELIKLFFIPVPSIEEQLKIIVKYNKLLTQLTLYKNIFLYIFPLYIPVNIWYLRFLVNKTIHFSTLFYIKILNMLKNVKFLWIFTQLTLFMQLVLERP" }, "paralog": false, "identity": 0.9902912621359223, "score": 597.0, "evalue": 2.16e-219, "description": "Internal stop codon at 641261, 641900, 641906. Nonsense mutation around 641261, 641900, 641906.", "psc": { "uniref90_id": "UniRef90_C4XDR5", "product": "Type I restriction modification DNA specificity domain-containing protein", "uniref50_id": "UniRef50_C4XDR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDR5", "UniRef:UniRef50_C4XDR5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDR5" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "EFPKPENHBP_1197", "locus": "GCA000186005_01170" }, { "type": "cds", "sequence": "contig_1", "start": 641904, "stop": 642185, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTAATAGACTTACAGCAGAACAATTGAGAAAATCTATTCTTCAATTGGCTATTCAAGGAAAATTAGTTAAACAAGATCCAAATGATGAGCCTGCTAGCAAACTTATTGAGAGAATTTATCAAGAAAAACAAAAATTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTAAAGGTGAGGATAATCATTATTATGAGAAGATAGGCAAAAATATTAAAGATATTACTGAAGAATTGCCTTTTGAAATACCAGAGAATTGA", "aa": "MKINRLTAEQLRKSILQLAIQGKLVKQDPNDEPASKLIERIYQEKQKLIKEGKIKKDKQESFIFKGEDNHYYEKIGKNIKDITEELPFEIPEN", "aa_hexdigest": "086a9e3314cbc420a472e1686bec0810", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002ED51773", "query_cov": 0.9247311827956989, "subject_cov": 1.0, "identity": 0.628, "score": 103.0, "evalue": 3.61e-27, "valid": false, "uniref50_id": "UniRef50_K1U594" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10984.550299999992, "isoelectric_point": 8.752953147888185 }, "id": "EFPKPENHBP_1198", "locus": "GCA000186005_01171" }, { "type": "cds", "sequence": "contig_1", "start": 642499, "stop": 643284, "strand": "-", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1V4Q7" ], "nt": "ATGAAGGACGGTGTTTTATTAAGACAAGTTATTAATGTAATTAATGAATTAGATTTTGCTGACTATAAAGAAAAACATGAGTTTGGTACTATATATGAAACTATTTTAAAAAGCTTGCAAAGTGCTGGAAATGCAGGTGAATTTTATACACCAAGAGCAGTAACTGATTTTATGGTTAAAATGATTAATCCAAAATTAGGAGAAAAAATTGCAGATTTTGCTTGCGGAACAGGTGGATTCTTAACATCTTCATTGAAGCATTTAGAGGATCAAAAGAAAACAGTTGAAGATGAAAACTTATATGATAATTCAGTCTATGGCATTGAGAAAAAACCATTGCCATATCTTTTATGTATTACTAATATGTTAATTCATGATGTAGATGAACCAAAAATATTTCATGATAATTCTTTAGAAAAAAGAGTACAAGATTATACTGAAGCTGATAAATTCGACATTATCTTAATGAATCCCCCATATGGTGGTTCAGAAAAAGAAGCTGTAAAAAATAATTTTCCAGCTGATTTAAGAAGTAGTGAAACTGCAGATTTATTTATGAACGTTATTATGTATCGTTTAAAGAAAAAAGGACGTTGTGCAGTTATATTGCCTGATGGTTTTTTATTTGGAGCAGATAATGCAAAAGTTGCAATAAAAACTAAATTATTAAAAGAATTTAATTTACACACCATTATTAGAATGCCGCATAGTGTTTTTGCTCCATATACTTCAATAACAACAAATATTTTGTTTTTTGATCACACTCATCCAACTGAAGAAGTTTGA", "aa": "MKDGVLLRQVINVINELDFADYKEKHEFGTIYETILKSLQSAGNAGEFYTPRAVTDFMVKMINPKLGEKIADFACGTGGFLTSSLKHLEDQKKTVEDENLYDNSVYGIEKKPLPYLLCITNMLIHDVDEPKIFHDNSLEKRVQDYTEADKFDIILMNPPYGGSEKEAVKNNFPADLRSSETADLFMNVIMYRLKKKGRCAVILPDGFLFGADNAKVAIKTKLLKEFNLHTIIRMPHSVFAPYTSITTNILFFDHTHPTEEV", "aa_hexdigest": "5ac17f212597497eccde84c953af62dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003461D892", "query_cov": 0.8275862068965517, "subject_cov": 1.0, "identity": 0.6940000000000001, "score": 325.0, "evalue": 3.53e-110, "valid": false, "product": "class I SAM-dependent DNA methyltransferase", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0AAJ1V4Q7", "kegg_orthology_id": "K03427" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1V4Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1V4Q7" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "EFPKPENHBP_1199", "locus": "GCA000186005_01172" }, { "type": "cds", "sequence": "contig_1", "start": 643582, "stop": 643677, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTGGAAATTTAATTAAAAGAATTCAAGATATTATGAGAAACGATGCAGGTGTTGATGGCGATGCTCAACGTATTAGTCAAATGACTTGA", "aa": "MAIGNLIKRIQDIMRNDAGVDGDAQRISQMT", "aa_hexdigest": "0be4c54aad361adb3bac644183bc58d7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3431.9204999999993, "isoelectric_point": 5.854097938537596 }, "id": "EFPKPENHBP_1200", "locus": "GCA000186005_01173" }, { "type": "cds", "sequence": "contig_1", "start": 643696, "stop": 644058, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAGGCATTTTAATTGAAAACTTAAGAAATATTTATCCAAGAGATTTTGATGATTTCGATTTAATCTGTAGCATTGCATATGGATCAAAATTACTAAAAAGATCTGAAAGAGTAAATAATAAAAAAGTTGTTCAAATAATAGAAAGACAAAATGTTGAAACATGTAGAAAAATTTTACAAATTCTTATAAATAAATATCAAGAAGAAGTCATTGATGAATTAACAGATAACAATAATTTGAGATTAAAAGAATTTGAACAATTTGGCGGTCCTTTAAAAATTGCTAAACTTTTTGGTGGAAAAGAACAATATATTCAGACTGTTAAAGAAATTCAAAAAGCACTTTATATAGTATAA", "aa": "MSKGILIENLRNIYPRDFDDFDLICSIAYGSKLLKRSERVNNKKVVQIIERQNVETCRKILQILINKYQEEVIDELTDNNNLRLKEFEQFGGPLKIAKLFGGKEQYIQTVKEIQKALYIV", "aa_hexdigest": "a621653482aa9d1c3d44a020045661ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14093.229899999988, "isoelectric_point": 8.847399330139162 }, "id": "EFPKPENHBP_1201", "locus": "GCA000186005_01174" }, { "type": "cds", "sequence": "contig_1", "start": 644293, "stop": 644934, "strand": "-", "frame": 1, "gene": null, "product": "Type I restriction-modification enzyme R subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377ZNK3" ], "nt": "GTGGAAGACAATGAATACAATGTAAAGGATTTTGATAAAACTATTGTAATAGAAGAAAGAACAAAGGAGGTAGCTAAAAAAATTACCGATTATTTAAAATCAATTAACAATAGATATGCAAAAACAATTGTATTTTGTGACGACATAGAACATGCTGAGAGAATGAGACAAGCTTTAGTTCACTACAATAGTGATATGATGGCCAAAGATCATAGATATATTGTTCGTATTACTGGTGACAATGAAGAAGGTAAAAAACAATTAGATAATTTCATTGATGATAATGAGCTATATCCAACAATAGTTACAACCAGTGAACTTATGACTACAGGTGTCAATTGTAAATTGTGTCGTGTTATTGCACTTGATAATACTTTTGGTGAAAATGGTATGACTAAATTTAAACAAATAATTGGCCGAGGCACCAGAATTAAAGAATTTGATGATCCTAAAGATAAACATAAAAACAAATTGTATTTCACTATTTTAGACTTTAAAAATGCTACTAAATTATTTGCTGATCCTGATTTTAATGGCCCAGCAATCCAAGAAGAATTTTTTAATCCTGAAATTCTTAGAAATAAAAAAGCAAAACAAGAAACAAAAAAAGAATATGAATTAGTTGATGAGGGATTAGTCTGA", "aa": "MEDNEYNVKDFDKTIVIEERTKEVAKKITDYLKSINNRYAKTIVFCDDIEHAERMRQALVHYNSDMMAKDHRYIVRITGDNEEGKKQLDNFIDDNELYPTIVTTSELMTTGVNCKLCRVIALDNTFGENGMTKFKQIIGRGTRIKEFDDPKDKHKNKLYFTILDFKNATKLFADPDFNGPAIQEEFFNPEILRNKKAKQETKKEYELVDEGLV", "aa_hexdigest": "a5e57a0eeff3a2d57b4c593c32ad93f3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A377ZNK3", "query_cov": 0.8544600938967136, "subject_cov": 0.9479166666666666, "identity": 0.56, "score": 189.0, "evalue": 1.55e-57, "valid": false, "product": "Type I restriction-modification enzyme R subunit", "uniref50_id": "UniRef50_A0A377ZNK3" }, "pscc": { "uniref50_id": "UniRef50_A0A377ZNK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377ZNK3" ], "product": "Type I restriction-modification enzyme R subunit" }, "genes": [], "id": "EFPKPENHBP_1202", "locus": "GCA000186005_01175" }, { "type": "cds", "sequence": "contig_1", "start": 645172, "stop": 645408, "strand": "-", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0031B703C8" ], "nt": "TTGATTGATCAAACTATAATAGGGGATTTTAAACCTTTCAAAAATAGTATGGTTAAAGTTTCAAAAAGAAATATGGACACATCTTATGAAATTTATTTATCTCTATATCAACAATTATCTGAAAATGGCTCCGAAGATTCACTCAAAAAATTAAAAGAAAGCTTTAAACCAGACTTTTTCGATTTAATAATTGTTGATGAATGTCACAGAGGTAGTGCAAGTGAAGATTCTAAATGA", "aa": "MIDQTIIGDFKPFKNSMVKVSKRNMDTSYEIYLSLYQQLSENGSEDSLKKLKESFKPDFFDLIIVDECHRGSASEDSK", "aa_hexdigest": "595984c7b02e00fba0ae456d81d14e2e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00300492D9", "query_cov": 0.9871794871794872, "subject_cov": 0.9058823529411765, "identity": 0.5579999999999999, "score": 91.3, "evalue": 1.45e-22, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI0031B703C8" }, "pscc": { "uniref50_id": "UniRef50_UPI0031B703C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0031B703C8" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "EFPKPENHBP_1203", "locus": "GCA000186005_01176" }, { "type": "cds", "sequence": "contig_1", "start": 645457, "stop": 645987, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13588.12" ], "nt": "ATGGAATATTATATTTCTGCTGGAAAAGTGATTTTCGAAAATGGTGAGGCTAAAAAACTAAAAGCAAAAAGAATTGATTATTTGTTGAATTACAATGGAGATGGTTCACCGCTCGCAATTATTGAAGCAAAAGGTGAAGATCATAAAGAAGGCGACGGATTACTCCAAGCTCAAAATTATGCTAAAAGGTTGGGCGTTCGTTTTGTTTTTACATCAAATGGTAACAATTTTCGATTTTATGATATGAAAACAGGAATAGAGAAAACAATAGGGTTAGATGAATTTTTCACGCCCGATGAATTATTTGAGATGACATATGGTGAAGAAATCAAGGAAAATAAGGCTTTAGAAAAAATAATTTCCACTCCTTATTATTATGGACAAAATTCTCATATTCCTAGATATTATCAAAAAAGAGCCATAAATGATACAGTAGACGCTGTTGCTAAAGGAAAAGATAGAATTTTACTAGTTATGGCCACTGGCACAGGTAAAACATATGTTGCTTTTCAAATAATCTGGAGATTGTGA", "aa": "MEYYISAGKVIFENGEAKKLKAKRIDYLLNYNGDGSPLAIIEAKGEDHKEGDGLLQAQNYAKRLGVRFVFTSNGNNFRFYDMKTGIEKTIGLDEFFTPDELFEMTYGEEIKENKALEKIISTPYYYGQNSHIPRYYQKRAINDTVDAVAKGKDRILLVMATGTGKTYVAFQIIWRL", "aa_hexdigest": "dc17bafc6ae91c0dfe2829e24a1fbdf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13588.12", "name": "HSDR_N_2", "length": 76, "aa_cov": 0.42613636363636365, "hmm_cov": 0.9868421052631579, "evalue": 1.659214942623701e-08, "score": 30.914335250854492, "start": 20, "stop": 94 } ], "seq_stats": { "molecular_weight": 20151.709899999994, "isoelectric_point": 7.782317161560059 }, "id": "EFPKPENHBP_1204", "locus": "GCA000186005_01177" }, { "type": "cds", "sequence": "contig_1", "start": 646384, "stop": 646716, "strand": "+", "frame": 1, "gene": null, "product": "protein adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "nt": "ATGAAAAATTCAATAGATCAAAAAAGTAAAATAAAGGCTTATAACTTATTTGACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCCGAAAGTAATTTGGAGCAAATAATAGATAAATATATTGAAATGAATATAGCGCATCCATTTCTAGAAGGCAATGGACGAAGTACTAGAATTTGA", "aa": "MKNSIDQKSKIKAYNLFDLIIEDKLEIGTTKSLMQIHGYLFGGLYSFAGQIRKKDISKGGFRFANSTYLNETLKNIENMPESNLEQIIDKYIEMNIAHPFLEGNGRSTRI", "aa_hexdigest": "973984e6cb6815f6a28c7ffd5ddc9218", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6G5QHC3", "query_cov": 1.009090909090909, "subject_cov": 0.8473282442748091, "identity": 0.613, "score": 132.0, "evalue": 7.2e-38, "valid": false, "product": "protein adenylyltransferase", "uniref50_id": "UniRef50_A0A6G5QHC3" }, "pscc": { "uniref50_id": "UniRef50_A0A6G5QHC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "product": "protein adenylyltransferase" }, "genes": [], "id": "EFPKPENHBP_1205", "locus": "GCA000186005_01178" }, { "type": "cds", "sequence": "contig_1", "start": 646795, "stop": 646920, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ], "nt": "ATGCAAGAGAGCCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGGATTGATTATTCTTACTACTATGAAGAGTAA", "aa": "MQESPYDDTKIKNLIKKSLTDKINDRELFMKGIDYSYYYEE", "aa_hexdigest": "c7979606339603708649a7e1e6577ae1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A134CFU1", "query_cov": 1.0, "subject_cov": 0.803921568627451, "identity": 0.585, "score": 48.5, "evalue": 1.11e-06, "valid": false, "uniref50_id": "UniRef50_A0A134CFU1" }, "pscc": { "uniref50_id": "UniRef50_A0A134CFU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.603400000001, "isoelectric_point": 4.920460700988768 }, "id": "EFPKPENHBP_1206", "locus": "GCA000186005_01179" }, { "type": "cds", "sequence": "contig_1", "start": 647293, "stop": 647526, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATTAAACCTAAGGAATTATTCGACAAAGTTAAAGAAGCAATTAAAAATAAAGAAGTAGGTGACAAAAGTGACTATAGATATAAACTAAAAGGTAATTTAGCAAATATTAAATATGCCAGATTATATAAAGATTTAATGGATATAAATAATATGATTCTTGCTAATTTATGTGATTTAGTTAGAAATGAAGATATATCTAAAAGTTCTTTAGGTGGTAAACCTTGA", "aa": "MKEIKPKELFDKVKEAIKNKEVGDKSDYRYKLKGNLANIKYARLYKDLMDINNMILANLCDLVRNEDISKSSLGGKP", "aa_hexdigest": "fb181170c2c7abb9c6fc525910557e75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8877.295499999995, "isoelectric_point": 9.35205783843994 }, "id": "EFPKPENHBP_1207", "locus": "GCA000186005_01180" }, { "type": "cds", "sequence": "contig_1", "start": 648007, "stop": 648195, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTATTTAATATTATAACTTCAGATGACATTAATGATGAAGTTGGTTGCGACATCATGGAAGAATATGAATTAGAAATGTATGAAGAAGTTAGAAATTATATAAAACGCGAAATAGGCAACAAAAAAACATCTAAAGTAGTGGCTTATCAACTTTGTGATTCAAAAGGTAAAAGTATTTGA", "aa": "MKKLFNIITSDDINDEVGCDIMEEYELEMYEEVRNYIKREIGNKKTSKVVAYQLCDSKGKSI", "aa_hexdigest": "a31bd328f002aa5802a348a92729451f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7269.242900000002, "isoelectric_point": 4.850491905212402 }, "id": "EFPKPENHBP_1208", "locus": "GCA000186005_01181" }, { "type": "cds", "sequence": "contig_1", "start": 648523, "stop": 649125, "strand": "+", "frame": 1, "gene": null, "product": "HTH rpiR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0", "UniRef:UniRef90_C4XDS0" ], "nt": "TTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAGTTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCCAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATAAATTCGATCCATGATTTTAAGGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGATAAAAGCAATTTTAATTTTCAAATATTGACAAGAAACGAAAATGTTTCTTATGCAAATTGCACCTCACCAATAATTTATGATTATATTCCTGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTCATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAA", "aa": "MQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCINSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK", "aa_hexdigest": "226807ccab47cad368ce4d053dd09b1e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 648322, "stop": 649125, "observations": { "mutation": [ 648397 ], "stop": [ 648397 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS0", "query_cov": 1.0, "subject_cov": 0.7490636704119851, "identity": 0.995, "score": 389.0, "evalue": 1.01e-135, "gene_start": 1, "gene_end": 200, "reference_start": 68, "reference_end": 267, "reference_sequence": "MMKRIKIFDKKLVENYKDTYSYLIKWIEENPHEFLACSQHQITEKIYVSQSSLSRFTKFLGFESYRELQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCINSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK" }, "paralog": false, "identity": 0.9925093632958801, "score": 525.0, "evalue": 1.46e-191, "description": "Internal stop codon at 648397. Nonsense mutation around 648397.", "psc": { "uniref90_id": "UniRef90_C4XDS0", "product": "HTH rpiR-type domain-containing protein", "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS0", "UniRef:UniRef50_C4XDS0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0" ], "product": "HTH rpiR-type domain-containing protein" } }, "id": "EFPKPENHBP_1209", "locus": "GCA000186005_01182" }, { "type": "cds", "sequence": "contig_1", "start": 649279, "stop": 649440, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTAAGTAATAATTACAACCGTAATTTTGCTTGA", "aa": "MKEKLVKYLKMTIVIFLAGSLIGIYFLKTGQFENHSQKILLDRLSNNYNRNFA", "aa_hexdigest": "a38f002e3941c1695047e633cfac5783", "start_type": "GTG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6223.3135999999995, "isoelectric_point": 9.90029697418213 }, "id": "EFPKPENHBP_1210", "locus": "GCA000186005_01183" }, { "type": "cds", "sequence": "contig_1", "start": 649609, "stop": 649782, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAACAGTTACGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGCTGATTTAGAAGACAACAAGAAAAAAATACAAGAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGACGGAATTTAA", "aa": "MLLTVTYVPKVQKKLVIADLEDNKKKIQEDNQKLKEAISFKKKEEVVSEQETYEDGI", "aa_hexdigest": "af3c8b88a027043851a269e600c6744a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6653.6034, "isoelectric_point": 5.384835243225097 }, "id": "EFPKPENHBP_1211", "locus": "GCA000186005_01184" }, { "type": "cds", "sequence": "contig_1", "start": 649792, "stop": 650364, "strand": "+", "frame": 1, "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "db_xrefs": [ "EC:5.3.1.17", "GO:0008697", "GO:0045490", "KEGG:K01815", "SO:0001217", "UniRef:UniRef50_A0A449A2L5", "UniRef:UniRef90_C4XDS2" ], "nt": "ATGAGATTTAAAACAACATATGCAGTTTCAGCAAATGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGCGGAAAGCTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACGCCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTCTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTTAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGCCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAAGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGATTACAATTTTAGAACCTAACAATATGTGA", "aa": "MRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGITILEPNNM", "aa_hexdigest": "b99137388033af85f29693fce6dc1463", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "kduI" ], "truncated": "3-prime", "pseudogene": { "start": 649789, "stop": 650631, "observations": { "mutation": [ 650362, 650518, 650563 ], "stop": [ 650362, 650518, 650563 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS2", "query_cov": 1.0, "subject_cov": 0.6785714285714286, "identity": 0.995, "score": 380.0, "evalue": 3.38e-132, "gene_start": 1, "gene_end": 190, "reference_start": 2, "reference_end": 191, "reference_sequence": "MMRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGLTILEPNNMWNTMPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAPWSIHSGVGTSNYAFIWAMGGENLDYTDMQGVKTIDLK" }, "paralog": false, "identity": 0.9821428571428571, "score": 555.0, "evalue": 1.06e-203, "description": "Internal stop codon at 650362, 650518, 650563. Nonsense mutation around 650362, 650518, 650563.", "psc": { "uniref90_id": "UniRef90_C4XDS2", "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "ec_ids": [ "5.3.1.17" ], "uniref50_id": "UniRef50_A0A449A2L5", "kegg_orthology_id": "K01815", "go_ids": [ "GO:0008697", "GO:0045490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS2", "EC:5.3.1.17", "UniRef:UniRef50_A0A449A2L5", "KEGG:K01815", "GO:0008697", "GO:0045490" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2L5" ], "product": "4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase" } }, "id": "EFPKPENHBP_1212", "locus": "GCA000186005_01185" }, { "type": "cds", "sequence": "contig_1", "start": 650371, "stop": 650520, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGTCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGA", "aa": "MPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAP", "aa_hexdigest": "e3d1cba795fd1407655ae716ebcf170c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5662.5078, "isoelectric_point": 6.891182136535646 }, "id": "EFPKPENHBP_1213", "locus": "GCA000186005_01186" }, { "type": "cds", "sequence": "contig_1", "start": 650631, "stop": 650828, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAGAAATCTAAATAATTCATTAATCTATGAAAAAGAATTCAATGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGA", "aa": "MFRNLNNSLIYEKEFNDTKLLTKEEIKKALDLIIKQVDQNIDYFGKEFMIANTFNNKYQKIYSSE", "aa_hexdigest": "4f717bc35b55e86fe7931c661e90bf37", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.878000000002, "isoelectric_point": 6.243842887878418 }, "id": "EFPKPENHBP_1214", "locus": "GCA000186005_01187" }, { "type": "cds", "sequence": "contig_1", "start": 650967, "stop": 651104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGA", "aa": "MGFLYSPSCVAAYKLTKNQEAKEAALLAADNLISRYQEKGKFIQA", "aa_hexdigest": "008699fd48050ab2f7dac701666d2cec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4981.7018, "isoelectric_point": 8.889368247985843 }, "id": "EFPKPENHBP_1215", "locus": "GCA000186005_01188" }, { "type": "cds", "sequence": "contig_1", "start": 651588, "stop": 651731, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGA", "aa": "MLKITQKDYKTDLYEKSAHLILRNLINKYHNKKIEPGTPILLHGVYS", "aa_hexdigest": "a2005db7a4beef07139611715a016d0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5539.4529999999995, "isoelectric_point": 9.573958015441892 }, "id": "EFPKPENHBP_1216", "locus": "GCA000186005_01189" }, { "type": "cds", "sequence": "contig_1", "start": 651909, "stop": 652052, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGA", "aa": "MQVNGSNSVIVANDEAAVSETQQQLMKVTVPEGVRCSFYSVDKLIEI", "aa_hexdigest": "fdbc5a0ad85c6edb553bf231e3ff5b5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.764699999999, "isoelectric_point": 4.3588346481323255 }, "id": "EFPKPENHBP_1217", "locus": "GCA000186005_01190" }, { "type": "cds", "sequence": "contig_1", "start": 652405, "stop": 652512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGA", "aa": "MGTYTATPIVYALGVGIILGDIQTAVIVGAAGQTV", "aa_hexdigest": "850023f892d116746b7ff6b3a76fe1a3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3434.9936999999995, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_1218", "locus": "GCA000186005_01191" }, { "type": "cds", "sequence": "contig_1", "start": 652678, "stop": 652887, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGA", "aa": "MQLLTTLLFTLMSPISEIAKKQITKGKFKTFKFLSHVTILVLMIITFGFGLLVGVSANGIARFSSSIPD", "aa_hexdigest": "642d306918ad83035b34f00046d454a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7562.090899999997, "isoelectric_point": 10.294907951354979 }, "id": "EFPKPENHBP_1219", "locus": "GCA000186005_01192" }, { "type": "cds", "sequence": "contig_1", "start": 652918, "stop": 653211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAA", "aa": "MLPALGFALILKVMLKKEYIGFALLGYFLALVFEVISKITGQAFSVLALALTAMAFVLIIISIKKLAGIKEAAGVARNISNTSSNNENNKGGQQDGI", "aa_hexdigest": "dfcdad617cfb3ed09a556a990b2ab440", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10287.194299999986, "isoelectric_point": 9.631657218933103 }, "id": "EFPKPENHBP_1220", "locus": "GCA000186005_01193" }, { "type": "cds", "sequence": "contig_1", "start": 653528, "stop": 653950, "strand": "+", "frame": 2, "gene": null, "product": "PTS system mannose/fructose/sorbose family IID component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7", "UniRef:UniRef90_C4XDS6" ], "nt": "ATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATTGGTTTTGCTCAAAGCGGAAATATACTAGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATCAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTTTAGACAACATTATGCCACTATTTGCTTCAGGAATATGA", "aa": "MSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGI", "aa_hexdigest": "6bb174903953e55ef2f94aae8d55bd27", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 653228, "stop": 654064, "observations": { "mutation": [ 653264, 653504, 653948, 653987 ], "stop": [ 653264, 653504, 653948, 653987 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS6", "query_cov": 1.0, "subject_cov": 0.4713804713804714, "identity": 1.0, "score": 255.0, "evalue": 2.11e-83, "gene_start": 1, "gene_end": 140, "reference_start": 120, "reference_end": 259, "reference_sequence": "MMKITREVNKMESNNKDFVQVNQAQTKKLSIWTYIIVALRSYFLQSGFNYANFQGLGYANIIYPALKKIYGYGTQQFKDALLNNIEFYNTNPQCIPLITSIHIQLLNNGTSWADARTIKMSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGIWVIFIYFMVTKYKWSPYKVIVVTILVGVTAGVFGILGV" }, "paralog": false, "identity": 0.9856115107913669, "score": 520.0, "evalue": 2.89e-190, "description": "Internal stop codon at 653264, 653504, 653948, 653987. Nonsense mutation around 653264, 653504, 653948, 653987.", "psc": { "uniref90_id": "UniRef90_C4XDS6", "product": "PTS system mannose/fructose/sorbose family IID component", "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS6", "UniRef:UniRef50_A0A449A2J7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7" ], "product": "PTS system mannose-specific EIID component" } }, "id": "EFPKPENHBP_1221", "locus": "GCA000186005_01194" }, { "type": "cds", "sequence": "contig_1", "start": 654064, "stop": 654471, "strand": "+", "frame": 1, "gene": null, "product": "PTS EIIA type-4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354709.1", "SO:0001217", "UniParc:UPI0001E32E8F", "UniRef:UniRef100_A0AB32XCB1", "UniRef:UniRef50_C4XDS7", "UniRef:UniRef90_C4XDS7" ], "nt": "ATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGGTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAA", "aa": "METKILVIGHSQFPRGVVEGLKFVSGIEEDIDYKYIDNDHSIDELKNDLEKYLEKCENIIIFADLPGGSPHRLAVETIEKTKKKNAIVIAGAGMPMIMDIAFKVILIKFKSFDEIICFIGEKKKNLDKYIMITGA", "aa_hexdigest": "61f2c5627429de0f7932c21002b0d7d5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32E8F", "ncbi_nrp_id": "WP_013354709.1", "uniref100_id": "UniRef100_A0AB32XCB1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E8F", "RefSeq:WP_013354709.1", "UniRef:UniRef100_A0AB32XCB1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCB1", "uniref90_id": "UniRef90_C4XDS7" }, "psc": { "uniref90_id": "UniRef90_C4XDS7", "product": "PTS EIIA type-4 domain-containing protein", "uniref50_id": "UniRef50_C4XDS7" }, "pscc": { "uniref50_id": "UniRef50_C4XDS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS7" ], "product": "PTS EIIA type-4 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1222", "locus": "GCA000186005_01195" }, { "type": "cds", "sequence": "contig_1", "start": 654474, "stop": 654809, "strand": "+", "frame": 3, "gene": "yajC", "product": "Preprotein translocase subunit YajC", "db_xrefs": [ "KEGG:K03210", "RefSeq:WP_013354710.1", "SO:0001217", "UniParc:UPI0001E32E90", "UniRef:UniRef100_C4XDS8", "UniRef:UniRef50_C4XDS8", "UniRef:UniRef90_C4XDS8" ], "nt": "ATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGACGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAA", "aa": "MDLAVKIVLFILLGLFILQIIVVTVILPILKKKRDEKIAREHKELVKNIKSGHEVLLQNGIYGTYVNSSGDETFNMRINEENNVIIKVNKSAILGFFDKDIAKKIEQRKQK", "aa_hexdigest": "b6902950ec64bb2a671c1fd0db03b859", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32E90", "ncbi_nrp_id": "WP_013354710.1", "uniref100_id": "UniRef100_C4XDS8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E90", "RefSeq:WP_013354710.1", "UniRef:UniRef100_C4XDS8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDS8", "uniref90_id": "UniRef90_C4XDS8" }, "psc": { "uniref90_id": "UniRef90_C4XDS8", "product": "Preprotein translocase subunit YajC", "uniref50_id": "UniRef50_C4XDS8", "kegg_orthology_id": "K03210" }, "pscc": { "uniref50_id": "UniRef50_C4XDS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS8" ], "product": "Preprotein translocase subunit YajC" }, "genes": [ "yajC" ], "id": "EFPKPENHBP_1223", "locus": "GCA000186005_01196" }, { "type": "cds", "sequence": "contig_1", "start": 654822, "stop": 655163, "strand": "+", "frame": 3, "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "db_xrefs": [ "EC:1.1.1.127", "SO:0001217", "UniRef:UniRef50_A0A975S3K9", "UniRef:UniRef90_C4XDS9" ], "nt": "ATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAGGACAAAGTTGTTATTATTACAGGTGCAAACATGGGATTAGGTTTAGCTTATGCTAAAGCTTTATCAAATTGTGGCGCCAAATTAGTTATTAGTCATTTTGACACAAATACAAAAGAAATAGAAAACTTTTGTGCTAAAAACAAAACAGAATTATTGCTTGTTCAAGGTGACTTAACTAAAGAAGCAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGCACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGA", "aa": "MDFKFNKDYFSVKDKVVIITGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKEADRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKS", "aa_hexdigest": "fa7ef4aaa710400d80d15db49a94e0ac", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "kduD" ], "truncated": "3-prime", "pseudogene": { "start": 654822, "stop": 655466, "observations": { "mutation": [ 655161, 655197 ], "stop": [ 655161, 655197 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS9", "query_cov": 1.0, "subject_cov": 0.4396887159533074, "identity": 1.0, "score": 224.0, "evalue": 2.75e-72, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MDFKFNKDYFSVKDKVVIITGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKEADRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKSWNKVIDINMNAVWFLSQKVAQIMKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGHWAKPEEIAGAVVFLCSKASDYISGAIIPVDGGFLCR" }, "paralog": false, "identity": 0.9906542056074766, "score": 409.0, "evalue": 1.17e-147, "description": "Internal stop codon at 655161, 655197. Nonsense mutation around 655161, 655197.", "psc": { "uniref90_id": "UniRef90_C4XDS9", "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "ec_ids": [ "1.1.1.127" ], "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS9", "EC:1.1.1.127", "UniRef:UniRef50_A0A975S3K9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A975S3K9" ], "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD" } }, "id": "EFPKPENHBP_1224", "locus": "GCA000186005_01197" }, { "type": "cds", "sequence": "contig_1", "start": 655227, "stop": 655487, "strand": "+", "frame": 3, "gene": null, "product": "2-keto-3-deoxygluconate oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "nt": "ATGAAAAAGCAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGA", "aa": "MKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGH", "aa_hexdigest": "c17948bc9b73689f5eed682bff6a069d", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0015F461D0", "query_cov": 0.8488372093023255, "subject_cov": 0.9733333333333334, "identity": 0.589, "score": 88.6, "evalue": 1.76e-21, "valid": false, "product": "SDR family NAD(P)-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A377BY30" }, "pscc": { "uniref50_id": "UniRef50_A0A377BY30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "product": "2-keto-3-deoxygluconate oxidoreductase" }, "genes": [], "id": "EFPKPENHBP_1225", "locus": "GCA000186005_01198" }, { "type": "cds", "sequence": "contig_1", "start": 655586, "stop": 656083, "strand": "+", "frame": 2, "gene": null, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "db_xrefs": [ "EC:4.1.2.14", "EC:4.1.3.42", "KEGG:K01625", "SO:0001217", "UniRef:UniRef50_D5E5I3", "UniRef:UniRef90_C4XDT0" ], "nt": "ATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGA", "aa": "MSLSALDLIKKEKLIAVIRYNSYEEAKKAIETCIKGGIKIIEITLTTPEALKLIKEFSKSKVLIGAGTVVSHEDAYKSVKSGAKFLVSPHFSDSVNEVSQISEIPYLAGVMTPTEAISAYRQGSKLLKVFPSEIVGPNFISGLLKPFPFLELMPSGGVNLDNAAA", "aa_hexdigest": "1b6e50eb9980b0490f624c012d0f7917", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_C4XDT0", "query_cov": 1.0, "subject_cov": 0.8168316831683168, "identity": 1.0, "score": 316.0, "evalue": 1.55e-108, "valid": true, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "ec_ids": [ "4.1.2.14", "4.1.3.42" ], "uniref50_id": "UniRef50_D5E5I3", "kegg_orthology_id": "K01625" }, "pscc": { "uniref50_id": "UniRef50_D5E5I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5I3" ], "product": "Khg/kdpg aldolase" }, "genes": [], "id": "EFPKPENHBP_1226", "locus": "GCA000186005_01199" }, { "type": "cds", "sequence": "contig_1", "start": 656187, "stop": 656699, "strand": "+", "frame": 3, "gene": "pfkB", "product": "Carbohydrate kinase PfkB domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1", "UniRef:UniRef90_C4XDT1" ], "nt": "ATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGA", "aa": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTL", "aa_hexdigest": "8f957613d390c60272f158a2246db4d6", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkB" ], "truncated": "3-prime", "pseudogene": { "start": 656187, "stop": 657002, "observations": { "mutation": [ 656697, 656769 ], "stop": [ 656697, 656769 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT1", "query_cov": 1.0, "subject_cov": 0.5167173252279635, "identity": 1.0, "score": 332.0, "evalue": 8.45e-113, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTLWNNKYADFKKEIDPFLNLSDIVFGWISEQNYCKSNSSISAKDFDKLTCLIDKKYKNIELIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFEIER" }, "paralog": false, "identity": 0.992619926199262, "score": 531.0, "evalue": 1.15e-193, "description": "Internal stop codon at 656697, 656769. Nonsense mutation around 656697, 656769.", "psc": { "uniref90_id": "UniRef90_C4XDT1", "product": "Carbohydrate kinase PfkB domain-containing protein", "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT1", "UniRef:UniRef50_C4XDT1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1" ], "product": "Carbohydrate kinase PfkB domain-containing protein" } }, "id": "EFPKPENHBP_1227", "locus": "GCA000186005_01200" }, { "type": "cds", "sequence": "contig_1", "start": 656871, "stop": 657176, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00294.29" ], "nt": "TTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTAAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAAATTGAAAGGTAA", "aa": "MIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFEIER", "aa_hexdigest": "5164913bd6ea9f9a288e955f9f7c61ba", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00294.29", "name": "PfkB", "length": 51, "aa_cov": 0.49504950495049505, "hmm_cov": 1.0, "evalue": 1.1063108062203531e-08, "score": 31.062702178955078, "start": 21, "stop": 70 } ], "seq_stats": { "molecular_weight": 11662.07439999999, "isoelectric_point": 6.231167793273925 }, "id": "EFPKPENHBP_1228", "locus": "GCA000186005_01201" }, { "type": "cds", "sequence": "contig_1", "start": 657179, "stop": 658189, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATGGATCATCCTCTTGCTAACTTATATTATGCTGGGACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAGTAAAAGCTAAAAAAATTTTTGATCGAGAATTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGTCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCTGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGCAAAATTAAAAAATATTATTGAGAATGTTTTAGATGATTTTGCAAAAAATTATTATTACAGCGGACAACAACAATATGTTAACTGA", "aa": "MKLKKIILPLSMSAAIMPALSSSCTNLKSEGFDKYGNRIPSEINQLKNYLIFKERKVAIENTKASVAANAINNVENEDRYNQILSFLEPEFEILPKNFEYQYSAKVINKSEGDLLEINILVIQTSTGSKENIKLTLGQDTKFKKDSNLSKSDYEQILKNILIVNDLKYLVEEDESKLSDNDKKNKNNAVRFTDIILNKYNENLFMDHPLANLYYAGTYNNGTGIKATDSNEVKAKKIFDRELKAIEEAKELIYRKESQPVQDPETKRIVDEGYSDAQKVRVAIQKLLFLAAAYQIKPKGIFTNKFYQNAKLKNIIENVLDDFAKNYYYSGQQQYVN", "aa_hexdigest": "5222acf1ecc6d19b1106a586c0bd355d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38543.423000000024, "isoelectric_point": 8.490115547180178 }, "id": "EFPKPENHBP_1229", "locus": "GCA000186005_01202" }, { "type": "cds", "sequence": "contig_1", "start": 658301, "stop": 658999, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08124.17" ], "nt": "TTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAGCCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGACGGTTTTTATGTTGATGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTCCTATATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCCTAGAAATAAGGAAGATTGA", "aa": "MPNPRYGGAAPTAILAYKPVTKKRAQTGANAVDNAKPILISGILNEDTSKIEDCILSLYDNIFRDFVKKGDGFYVDGSYMQHTNIPYVGSYGEVLFTNIADIFKYFENTDLALGKDKRFEKIYQFIELSIMPFMFKGAISDGLSGRSIVRDDHSDLKKGLNILGSLAIIEKSASPIYKSRLREFIKNQISHLNKTQLQKLASEHKVRPLLIDALLEIKDLPDLPHPPRNKED", "aa_hexdigest": "62471aaada51bafc485f8b5451088f2f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08124.17", "name": "Lyase_8_N", "length": 169, "aa_cov": 0.728448275862069, "hmm_cov": 0.9822485207100592, "evalue": 1.9828297103603984e-40, "score": 135.17376708984375, "start": 18, "stop": 186 } ], "seq_stats": { "molecular_weight": 26091.72250000003, "isoelectric_point": 8.74012393951416 }, "id": "EFPKPENHBP_1230", "locus": "GCA000186005_01203" }, { "type": "cds", "sequence": "contig_1", "start": 659168, "stop": 659311, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTTCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGA", "aa": "MFNINLHGRISGFPEASVQENMDSYYQYDGSTFLATNNNYSPYSNKY", "aa_hexdigest": "4289bd61e48713dd358c909d4c653a7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5449.817700000001, "isoelectric_point": 4.74920482635498 }, "id": "EFPKPENHBP_1231", "locus": "GCA000186005_01204" }, { "type": "cds", "sequence": "contig_1", "start": 659636, "stop": 659860, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACGATTGAAAATAGAATGATCATTGATAAAAACACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAAAGGAACAAGATTTATTTATTTGA", "aa": "MGFVKAEVINYNNTLITDKVYFMVNGIIIVIGNTNIKNKLVNTTIENRMIIDKNTDMFSNQKVNINGKEQDLFI", "aa_hexdigest": "2919218f8863a3f368b4fd31959b0426", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8482.781299999999, "isoelectric_point": 8.007634162902832 }, "id": "EFPKPENHBP_1232", "locus": "GCA000186005_01205" }, { "type": "cds", "sequence": "contig_1", "start": 659927, "stop": 660367, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGACAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTCATAGCTTTAAAATATCAAAATAAAGATTCCTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATATGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCTGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGATTTAGATCTAAAAATTAATTTATGA", "aa": "MHEAKYPIITNMNSKKMVPVVRDFASLSINHEQNNTFMYAIVPNYEDSKKSNYLDILNNIKIIKNDQNFIALKYQNKDSLGQINDYYFVASLYENGNIKYKEQLNSLGSYDNEVSINYNEIPEFERNYKAYKNGLYLEDLDLKINL", "aa_hexdigest": "20624892c748cbec32c183ed6ffe1637", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17188.1405, "isoelectric_point": 5.3910306930542 }, "id": "EFPKPENHBP_1233", "locus": "GCA000186005_01206" }, { "type": "cds", "sequence": "contig_1", "start": 660380, "stop": 660634, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAAGAAAGTTCAAAATTCTTCAATTTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTATCAATTTAAGCGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGA", "aa": "MIIKKVQNSSILDVLVSNDLNENQYEIEFSKAFQNITSRKITNKKFSAKNVINLSEVTLNNVLGQSKIRVDNRLGEVNDYNHIS", "aa_hexdigest": "c46baadd3b26ba60394deb0c52212957", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.759499999996, "isoelectric_point": 9.048927497863769 }, "id": "EFPKPENHBP_1234", "locus": "GCA000186005_01207" }, { "type": "cds", "sequence": "contig_1", "start": 660663, "stop": 660773, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATAAAATTATATTTGCTTTAACTCCATTCACTCTCATGCCTTTAACTGCAATAAGTTGTAGTATTGAGAGTTCAAATGATTATGAAAACGCTAATAAGTGA", "aa": "MKKHKIIFALTPFTLMPLTAISCSIESSNDYENANK", "aa_hexdigest": "37792286e1ab56c56d51f2a437f292f6", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4057.710900000001, "isoelectric_point": 7.922987174987794 }, "id": "EFPKPENHBP_1235", "locus": "GCA000186005_01208" }, { "type": "cds", "sequence": "contig_1", "start": 660798, "stop": 661034, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGTGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTGGTTCAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTTAGATGAAACAACTACAAATTTTGACCAAAATTCTTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGA", "aa": "MKESENILINKLIKSEFKITNFYNEMILNKEFNINEVGSKITYNKNFKAFNNLDETTTNFDQNSFKTGKIVNLKKYFT", "aa_hexdigest": "05ac174fc673d49731b133c65794c07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9255.451099999995, "isoelectric_point": 9.188759422302244 }, "id": "EFPKPENHBP_1236", "locus": "GCA000186005_01209" }, { "type": "cds", "sequence": "contig_1", "start": 661077, "stop": 661286, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAACTAACGCTATTCAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAG", "aa": "MQSYFNPLSSKFADYVTEDTLYLKLTLFNSCALNLKTQHIIYAFTNSNKTGRLITKDNTLELQKLTQIS", "aa_hexdigest": "6bf9ac25f9c3518010822bd5738a8748", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.0176999999985, "isoelectric_point": 8.819097709655765 }, "id": "EFPKPENHBP_1237", "locus": "GCA000186005_01210" }, { "type": "cds", "sequence": "contig_1", "start": 661301, "stop": 661663, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATACTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGA", "aa": "MKKIKMILTLSSLTTCLPIIATSCKNEKEEKITNFDEVKSKLTFDVDNKSNIKASEFKDTNLIKINNLPSDFKKEITLENYEDGKIKFKLILIDKNNKKSQAYTITISGFKAETATPTIN", "aa_hexdigest": "88211fb482ff6b73c1ed9741f28ed660", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 85, "aa_cov": 0.6416666666666667, "hmm_cov": 0.9647058823529412, "evalue": 2.7398644079521625e-08, "score": 30.36296272277832, "start": 35, "stop": 111 } ], "seq_stats": { "molecular_weight": 13624.682299999977, "isoelectric_point": 9.148466682434083 }, "id": "EFPKPENHBP_1238", "locus": "GCA000186005_01211" }, { "type": "cds", "sequence": "contig_1", "start": 661835, "stop": 662113, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGA", "aa": "MTKDTSNSPVYTFEISNFEFTEEFDFVNFDEVASKYKELKLLTANKAEAKNVIAAYNATKSSSLDAKIKDNILVLMAGIFAKGKNSSKPQAS", "aa_hexdigest": "43177eb6ec6e342c5437a931d5148af9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10166.370999999994, "isoelectric_point": 6.232304573059082 }, "id": "EFPKPENHBP_1239", "locus": "GCA000186005_01212" }, { "type": "cds", "sequence": "contig_1", "start": 662348, "stop": 662539, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGA", "aa": "MLELIARTFATLSDTQKTANKTKLDILYNATKIFLPKASNLNSFKGEDANLSDGKNYKPTFNR", "aa_hexdigest": "1b942679f5eb2234a6ad148529c40b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7082.998200000002, "isoelectric_point": 9.630754661560058 }, "id": "EFPKPENHBP_1240", "locus": "GCA000186005_01213" }, { "type": "cds", "sequence": "contig_1", "start": 663563, "stop": 664225, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "EC:6.3.4.21", "KEGG:K00763", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_C4XDT5" ], "nt": "ATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGA", "aa": "MTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKL", "aa_hexdigest": "e0c4b595eac16f195cfadef4cc951529", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 663545, "stop": 664528, "observations": { "mutation": [ 664223 ], "stop": [ 664223 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT5", "query_cov": 1.0, "subject_cov": 0.6358381502890174, "identity": 1.0, "score": 435.0, "evalue": 2.8e-152, "gene_start": 1, "gene_end": 220, "reference_start": 7, "reference_end": 226, "reference_sequence": "MIRMVQMTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKLWGVRIDTSKNMVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKRK" }, "paralog": false, "identity": 0.9938837920489296, "score": 639.0, "evalue": 5.03e-235, "description": "Internal stop codon at 664223. Nonsense mutation around 664223.", "psc": { "uniref90_id": "UniRef90_C4XDT5", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "kegg_orthology_id": "K00763", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT5", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "KEGG:K00763" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "EFPKPENHBP_1241", "locus": "GCA000186005_01214" }, { "type": "cds", "sequence": "contig_1", "start": 664253, "stop": 664585, "strand": "+", "frame": 2, "gene": null, "product": "Nicotinate phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3", "UniRef:UniRef90_A0A3P8LBM3" ], "nt": "ATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAAAAAATAA", "aa": "MVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKKK", "aa_hexdigest": "3a733c58115d36a6586a4c368edd1341", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 664157, "stop": 664641, "observations": { "insertion": [ 664517, 664587 ], "mutation": [ 664223 ], "stop": [ 664223, 664560, 664594 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBM3", "query_cov": 0.8272727272727273, "subject_cov": 0.52, "identity": 0.835, "score": 150.0, "evalue": 2.78e-44, "gene_start": 1, "gene_end": 91, "reference_start": 33, "reference_end": 123, "reference_sequence": "MDYHNNVIEQALESYKVLGNKLWGVRIDTSKNMVDHMFDNQEENPEHYGVNIEQVKNLRSALDKVGANDVKIVVSSGFNAAKIKKFEEADTPVDSYGVGAGIFKFVSSFSADAVLLNGKKKQKRVANIDLIQNCKFTILKNNQFSKTPTKQSFWKIFIINNTLQKISKFKKLSII" }, "paralog": false, "identity": 0.75625, "score": 225.0, "evalue": 2.34e-76, "description": "Internal stop codon at 664223, 664560, 664594. Frameshift due to insertion around 664517, 664587. Nonsense mutation around 664223.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBM3", "product": "Nicotinate phosphoribosyltransferase", "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBM3", "UniRef:UniRef50_A0A3P8LBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3" ], "product": "Nicotinate phosphoribosyltransferase" } }, "id": "EFPKPENHBP_1242", "locus": "GCA000186005_01215" }, { "type": "cds", "sequence": "contig_1", "start": 664714, "stop": 665073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6", "UniRef:UniRef90_C4XDT6" ], "nt": "ATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGA", "aa": "MKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFT", "aa_hexdigest": "158bb4985583269e648457ca26a8d0c8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LIM6", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.6809999999999999, "score": 147.0, "evalue": 1.98e-43, "valid": false, "uniref50_id": "UniRef50_C4XDT6" }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 664651, "stop": 665130, "observations": { "mutation": [ 665071 ], "stop": [ 665071 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT6", "query_cov": 1.0, "subject_cov": 0.7484276729559748, "identity": 1.0, "score": 216.0, "evalue": 1.62e-70, "gene_start": 1, "gene_end": 119, "reference_start": 22, "reference_end": 140, "reference_sequence": "MLNNLIIKYNLNIIFLRRELVMKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFTWVLATIFLICTIVYAVRSK" }, "paralog": false, "identity": 0.9937106918238994, "score": 298.0, "evalue": 5.8e-105, "description": "Internal stop codon at 665071. Nonsense mutation around 665071.", "psc": { "uniref90_id": "UniRef90_C4XDT6", "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT6", "UniRef:UniRef50_C4XDT6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12818.22569999998, "isoelectric_point": 9.20713291168213 }, "id": "EFPKPENHBP_1243", "locus": "GCA000186005_01216" }, { "type": "cds", "sequence": "contig_1", "start": 665240, "stop": 665539, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGA", "aa": "MINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVE", "aa_hexdigest": "7b2f40e187a81d911ad9a1851c7c4198", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "3-prime", "pseudogene": { "start": 665162, "stop": 665800, "observations": { "mutation": [ 665537 ], "stop": [ 665537 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4669811320754717, "identity": 1.0, "score": 200.0, "evalue": 9.8e-64, "gene_start": 1, "gene_end": 99, "reference_start": 27, "reference_end": 125, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9905660377358491, "score": 414.0, "evalue": 2.87e-150, "description": "Internal stop codon at 665537. Nonsense mutation around 665537.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "EFPKPENHBP_1244", "locus": "GCA000186005_01217" }, { "type": "cds", "sequence": "contig_1", "start": 665543, "stop": 665800, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAA", "aa": "MNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK", "aa_hexdigest": "9c49cf9b22f296322c4146d5fc2768cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 665243, "stop": 665800, "observations": { "mutation": [ 665537 ], "stop": [ 665537 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4009433962264151, "identity": 1.0, "score": 167.0, "evalue": 4.48e-51, "gene_start": 1, "gene_end": 85, "reference_start": 128, "reference_end": 212, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9945945945945946, "score": 365.0, "evalue": 1.8e-131, "description": "Internal stop codon at 665537. Nonsense mutation around 665537.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "EFPKPENHBP_1245", "locus": "GCA000186005_01218" }, { "type": "cds", "sequence": "contig_1", "start": 665803, "stop": 666105, "strand": "+", "frame": 1, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "RefSeq:WP_013354736.1", "SO:0001217", "UniParc:UPI0001E32EA9", "UniRef:UniRef100_C4XDT8", "UniRef:UniRef50_C4XDT8", "UniRef:UniRef90_C4XDT8" ], "nt": "ATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAA", "aa": "MNLVTTNCGLNLSYSITENVFSQLIDQCFEEVKNVKLANEARISFDKEQTNVAFVIDIKIKKGSGLQQVIENLTQELESRCLGLLDIKPRSIRLCFVGYY", "aa_hexdigest": "d78de6b5c1f9791c3efd907a1110f8c4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EA9", "ncbi_nrp_id": "WP_013354736.1", "uniref100_id": "UniRef100_C4XDT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EA9", "RefSeq:WP_013354736.1", "UniRef:UniRef100_C4XDT8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDT8", "uniref90_id": "UniRef90_C4XDT8" }, "psc": { "uniref90_id": "UniRef90_C4XDT8", "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_C4XDT8" }, "pscc": { "uniref50_id": "UniRef50_C4XDT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT8" ] }, "genes": [], "id": "EFPKPENHBP_1246", "locus": "GCA000186005_01219" }, { "type": "cds", "sequence": "contig_1", "start": 666210, "stop": 666704, "strand": "-", "frame": 2, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_C4XDT9", "UniRef:UniRef90_C4XDT9" ], "nt": "ATGAAAATAAGTAGCGATGCTTCATTAATTGATACTCAATTGGCTAAAAGAGCAGGAACAATTCTTAAGTTAGTTGAATCAGTAAGTTCACACATGAAATTCGAAAAAGATGTTATGACACAAGTTGCAGCATACCGTTCAGCAGTTGCTACAATTCGAGAACAATCAGGAAATACTTCAGCAGATGCTATGGCAGTACGTCAAAACTTTGAAAGTCAAACAGAATCATTCTTAACAAGATTTATGGCTCAATTCGAAAAATATCCAGAACTTAAGAGTGCTGAATTAGTTAAAGGTTTAAATGATGAAATCGTTGAATGTGAAAACAACATTGCTTCAGCTAGAAGAATTTACAATACTTCAGCCACAACATTTAACCAAGAAATCTTTACATATCCAAAAAGTGTTGTTGCTTCAAGTATGAGATTAACAACAATTCTATTATTCCAAGCTAAAGAAGCTGATAGACAAGATGTTAGTGTGAAATTGTATTAA", "aa": "MKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY", "aa_hexdigest": "e8ec4ee496bed29dc3dfc5e235ceb26e", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "lemA" ], "truncated": "3-prime", "pseudogene": { "start": 666210, "stop": 666935, "observations": { "mutation": [ 666731, 666779 ], "stop": [ 666731, 666779 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT9", "query_cov": 1.0, "subject_cov": 0.6804979253112033, "identity": 1.0, "score": 301.0, "evalue": 3.52e-102, "gene_start": 1, "gene_end": 164, "reference_start": 78, "reference_end": 241, "reference_sequence": "MCIEHKNNYIEGEKMANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFIWYISFIFLIPIFIHISWVNYFRRQQMKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY" }, "paralog": false, "identity": 0.9875518672199171, "score": 459.0, "evalue": 7.58e-167, "description": "Internal stop codon at 666731, 666779. Nonsense mutation around 666731, 666779.", "psc": { "uniref90_id": "UniRef90_C4XDT9", "product": "LemA family protein", "uniref50_id": "UniRef50_C4XDT9", "kegg_orthology_id": "K03744", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT9", "UniRef:UniRef50_C4XDT9", "KEGG:K03744" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT9" ], "product": "LemA family protein" } }, "id": "EFPKPENHBP_1247", "locus": "GCA000186005_01220" }, { "type": "cds", "sequence": "contig_1", "start": 666777, "stop": 666893, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAATTTATACAATCGTGAAAACAGTCGTGCTATTAATGAAAAAGGCTATGAACCACGTTCAATGAGTACAGAAGTACCAGTTAAAGTAAACTTTTTTGGAAAATTTATTTGA", "aa": "MANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFI", "aa_hexdigest": "3130e5d19c8ce1b5d638747949276b32", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4438.0085, "isoelectric_point": 9.39608974456787 }, "id": "EFPKPENHBP_1248", "locus": "GCA000186005_01221" }, { "type": "cds", "sequence": "contig_1", "start": 666979, "stop": 667176, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE", "PFAM:PF02911.23" ], "nt": "ATGCAAGAAGCAATCAATATTATTAGAGCTTATGAGATGAATCCTGGTGCTTACACTTTTATTGATAATAAAAGAATTAAGATCTTTTTTGCAACCAAAAAAGAAATTAAAAATGTACCTAAATTAAAATTTAAAGATGGTTATTTATTTGCCACTGATTATCAATATGAATCTAAAAAACGAGTTAAGCTGGCATAG", "aa": "MQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA", "aa_hexdigest": "161e228b91d84bd13897f2a070d15771", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF014FE", "query_cov": 0.9846153846153847, "subject_cov": 0.810126582278481, "identity": 0.531, "score": 76.6, "evalue": 4.47e-17, "valid": false, "uniref50_id": "UniRef50_UPI002FF014FE" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF014FE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 33, "aa_cov": 0.5076923076923077, "hmm_cov": 1.0, "evalue": 7.360582034187748e-07, "score": 25.66942024230957, "start": 3, "stop": 35 } ], "seq_stats": { "molecular_weight": 7740.009099999999, "isoelectric_point": 9.776259803771971 }, "id": "EFPKPENHBP_1249", "locus": "GCA000186005_01222" }, { "type": "cds", "sequence": "contig_1", "start": 667288, "stop": 667815, "strand": "-", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_D3VRI0", "UniRef:UniRef90_C4XDU0" ], "nt": "ATGAAATTATTATTAGCCGGAACTCCTGCTTTTGCAGTTGAAATATTTGAAAAATTAATCAACAACTTTGAAGTTGTTGCAATTGTTTCGCAACCTGATCGCCCAAGTGTCAGAGGTCATAAATTAGCTCCAACGCCAACAAAATTATTAGCTCAAAAATATAACATTAAATGTTTTCAACCAGAAAAAATATCACAAATAAAAGATGAATTACAAACTTTAAATTATGACTATTTAATTACCTGTGCCTTTGGTCAATATATTCCTGAAAGTGTTTTAAATATTGCTAAAAAATTAAGTCTAAATATTCATGGTTCTTTACTTCCAAAATATCGTGGAGCAGCACCAATTCAATATTCACTTTTAAATAATGATCAAGAAACTGGTATTAGTCTAATGGAAATGATTAAACAAATGGATGCAGGTGATGTTTTTGTCCAAAAAGCCATTAAAATAGATGAATATGACACAGCAAGTACTTTATTTAATAAGCTTTCAAAGCTTAGTGCAGATAACATTGTGCAATGA", "aa": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQ", "aa_hexdigest": "82b0558bc26312d350f2db7090d9a928", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF2EE64", "query_cov": 0.9942857142857143, "subject_cov": 0.9560439560439561, "identity": 0.644, "score": 227.0, "evalue": 1.77e-73, "valid": false, "product": "methionyl-tRNA formyltransferase", "uniref50_id": "UniRef50_A0A3B0PK42" }, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 666985, "stop": 667815, "observations": { "mutation": [ 667290 ], "stop": [ 667290 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU0", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 1.0, "score": 342.0, "evalue": 1.43e-117, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQWLNDLDQNKLKRIKQDESKVTLSPKLEKEKALLNESLTMQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA" }, "paralog": false, "identity": 0.9963768115942029, "score": 537.0, "evalue": 7.9e-197, "description": "Internal stop codon at 667290. Nonsense mutation around 667290.", "psc": { "uniref90_id": "UniRef90_C4XDU0", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_D3VRI0", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU0", "EC:2.1.2.9", "UniRef:UniRef50_D3VRI0", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRI0" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "EFPKPENHBP_1250", "locus": "GCA000186005_01223" }, { "type": "cds", "sequence": "contig_1", "start": 668057, "stop": 668278, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAGGTATATTTGAACATGATAGCAATAATGTAAAAATTATTTATGTTGTTTCATTATTAGCACATATAATCATTCATATTACAATGATTGCTATTCTTGTTTATGCAATTAAAGCATTTGTAAAATCAATAGCTTCGAAAAATGTAATTAGCATTATTTTAAATATTTTGGTATTTGCGGCAGTTGTTTTAACAATTATTTGTTATGTTGGCTGA", "aa": "MIKGIFEHDSNNVKIIYVVSLLAHIIIHITMIAILVYAIKAFVKSIASKNVISIILNILVFAAVVLTIICYVG", "aa_hexdigest": "0a424a6c8e1bc6c805bd31726ec706d8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8023.816999999997, "isoelectric_point": 9.30312633514404 }, "id": "EFPKPENHBP_1251", "locus": "GCA000186005_01224" }, { "type": "cds", "sequence": "contig_1", "start": 668754, "stop": 668897, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTCAATAGCGGCGTATATTTTAATAAGATTATTTGATAAAAAACGTTTAGCTGACATTAGTGATTTTCTCGGTGAACGTTCTGTTGAAATTCATGAACAAGTTGAGCAAAGCAAAAAAGATCGTAACAAAATGTGA", "aa": "MASSIAAYILIRLFDKKRLADISDFLGERSVEIHEQVEQSKKDRNKM", "aa_hexdigest": "49879b9c837c8368de215a3a0fab766b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5481.2681, "isoelectric_point": 8.120969581604005 }, "id": "EFPKPENHBP_1252", "locus": "GCA000186005_01225" }, { "type": "cds", "sequence": "contig_1", "start": 669189, "stop": 669536, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATATCAACCTATTCAAAATTCAGCAAGTATCGAAATTGTTCAAAATAACCACGACTACAAATTCAATAGTGTAAGTAGTCGTGTTTATCGTGATCCAAAAAATCAAATATCACCTCGTGTTTATCGCGTCATCCGTGCTGAACAAATGCTTAAGATTTTAATCCTTTCTATTTCATTTGTAATTATGGCTGCTTCAATTATTCTCTTCACATTAGTCTATGCTAAAGTTGGTATCTTCAACAAAGAACAAAGATTAGTTGGCTACCTTGTACTTTTTGGATCAAGTGCCTTTATCTCAATGGCAATTGGTTTTAAAAATCTGATTGAAAATATTCAATGA", "aa": "MRKYQPIQNSASIEIVQNNHDYKFNSVSSRVYRDPKNQISPRVYRVIRAEQMLKILILSISFVIMAASIILFTLVYAKVGIFNKEQRLVGYLVLFGSSAFISMAIGFKNLIENIQ", "aa_hexdigest": "1f470b6d0be182ce1045367f9bd195de", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.433999999987, "isoelectric_point": 10.056503868103025 }, "id": "EFPKPENHBP_1253", "locus": "GCA000186005_01226" }, { "type": "cds", "sequence": "contig_1", "start": 669764, "stop": 672559, "strand": "-", "frame": 3, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "GO:0005524", "GO:0007059", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_Q98PK8", "UniRef:UniRef90_C4XDU3" ], "nt": "ATGGATGATGTTATCTTCGCTGGTTCTAAAACTGCAAAACCTATGGATAAAGCAGTAGTTACTTTAACTTTTGATAATCGAAATGGTATTAGTTCAATCCCGCATGAAACTATTTCAATTAGTCGTGTGCTTGAAAGAGGAAATGGAAACAACCAATATTTCTTAAATGGCGAACCATGTCGTCAAAAAGATATTAAAGATATTGCTATGGAAAGCGGTATTGGTAAAAGTTCATTAGCTATTATTTCTCAAGGTACAGTTAGTGATATTGCTGAAGCTACACCTGAAGAAAGAAAGTCTATTTTTGAAGAAGCTGCTGGTGTTTCAAAATACAAATTTCGTAAAAAAGAAGCTTTAAGCAAATTGGCTAAAACTCAAGAAGGTTTAGACCAAATTAAACTTGTTATTAGTGAAATTGAAAGAAAATTAATTCCATTAAGAAAACAAGCAGAAAAAGCTAAAGTTTACATTGCTAAAAGTGAAGAATTAAAAGCTGTTGAAGTTGGTCTTTTAGTAGACAACATCAAAACTTTTGGTGATATGTACGAAAGACTTGTAACTGAACTTGAAGGTGTTCAAGAAACTAAAAATGATCTTGATGCTCGTATTTCATCAGCTGAAGCTGCTATTACTCAAAATACTAAATTCAGATCAGAAGTTGAAGCCGAATTAAAAGAACTTTCAATTGAATATGAAACAGTTAAAGAAAGATTAAACAAATTAAACATTTCTTTAGCTAAAGAAAATGAACGTGAAAGATTAATTGCTGCTGGTGAAATTCAAGCAAACCAAGCTGAACAAATCGAAGCATACAAAACTATGCTTACAAGACATGCAAATAAATTAAATTACTACGCTCGTGAAATTGAAAATCAAGCAAACCAAATTGCTGAAAACCAAAAAACAATTTCAAAATATGACGAAGAAATTAATTTAATGAACATTAAAATTAACAAAAAACGTACAGACTTATTAAAAATTGAAACACAGTTAAACTTGCTTAACCAACAAAAAGAAAGTAACTCTTTATTATTTAAAGGGACCAAAAACATTTTAGAAAATAAAGCTTACTTTGGAAAAGCACTAAAAGGAACTGTAGCTGATTTAATTAAAGTTACAACAGAATATATTCCTGCTATTGACTCAATTTTAGCTAATGCTTCACAACACTTAGTGGTTGATAAAGCTGAAACAGCAGTTAAAGCTGTTAACTTCTTAAAAGATAATAATGGTGGTCGTGCTACATTTATTCCACTCACATCAATTCAACCAAAAATGATTCGTGATGATCATCTTTTAGCAATCCAAGGACACCCTGGATTTATTGATGTTGCAAGTAATTTAGTAACAATTGATCCTCAATATGAAATTCTTAATAAATTCCTTTTAGGTAATGTTATTGTTGCTAGCGACATTGAAAATGCTAACAAAATTTCAAGTATTCTCGAACGTCGTTACATGGTAGTTTCACTTGATGGTGACACCATTCGTTCAGGCGGTGTGATTATTGGTGGAGCTAAAAATACAGCTCAATCATTACTTGGATTTGATGAAAAAATTAAAGAGCTTGAAAATATTGTTCCAGGTATGAGAGCAGCAGTTCAAGCTGATGAATCAAAAATCAATGAAATAAGAAACTATCGTCGTAGTGCTCTTGAATTAGACAGCCAATTAAAACAACAAATTACTAGTCTAAAAACCCAAAAGAGCAGTGAACAAGGTCTTTACGACGAATTACAATTAAAACTTCAAAATATTACAAACAAAGAATTCAAAGTTAAAGAAGGTGTTTCAGCATCAAGCCCTGAAGCTTCAATTGAAGTTTTAGAAAGTCAAAAAATTGCTCTTGAATCACAATTCAGAGCTAAAAATGAACGTGTAAATACAATTGCTGCCGAAATCAATTCAACTTTAGCTCTTAAAAATGATTATGAAGCTACACGTCGTAAATTAACAGATGCTTTCTCAGCTAAAATTGGTGAAAGAGAAAGATCAAAAACTTTACTTGAACAAAGTAGAGAAAGACTTGCTTCTCAATACAAAATGACATTTGAAGCAGCTGAAAAAGTTCATCACTTAGAAATGAATCGTGATCAAGCTGAAGAAATTGTTCGTCAATTAAGAGAAGAAATTGAAGGCTTAGGTAACGTTAACTTAGAAGCTCTTGAACAATTAGTTGAAGAAGAAAAACGTTACAATGAATATGCCGAAGGTGAAAGAGAATTAAGTGCTGCAAAAGAAACACTTGAAAGCGCTATTGCAGACATGGATAAAATCATTATCACTCGTCTAACTAACATTATTAATGATGTTAATGATGAATTTAACAATGTCTTTAGAACAATGTTCGGTGGTGGTACTGCAAAATTATTTTTCAGCAACCCTAAAGATATTTTAGAATCAGGTGTTGAAATCGAAGCTCAACCTCCTGGAAAAAATATTAAAAACCTTAAATTATTCTCAGGCGGTGAAAAATCACTTATCGCTATCTCATTATTATTTGGTATTCTTAAAGCTCGTCCATTACCTCTTTGTATCCTTGACGAAGTTGAAGCTGCACTTGACGAAGCTAATGTTGTTCGTTATGCAGAATACCTTCAACAACTTAAAGAAAAAACTCAATTCTTGGTTATTACACACCGTCATGGAACAATGTCAAGAGTTGACACATTATTTGGTGCAACAATGCAAAACCGTGGTGTTACAAGCTTCTTCAGCATTCAATTAGCAGATGCTAAAAAACTTGTTGCAGACGAACAAAAAGAAGAATTAGAACCTTCAAACAAAAACAAATAA", "aa": "MDDVIFAGSKTAKPMDKAVVTLTFDNRNGISSIPHETISISRVLERGNGNNQYFLNGEPCRQKDIKDIAMESGIGKSSLAIISQGTVSDIAEATPEERKSIFEEAAGVSKYKFRKKEALSKLAKTQEGLDQIKLVISEIERKLIPLRKQAEKAKVYIAKSEELKAVEVGLLVDNIKTFGDMYERLVTELEGVQETKNDLDARISSAEAAITQNTKFRSEVEAELKELSIEYETVKERLNKLNISLAKENERERLIAAGEIQANQAEQIEAYKTMLTRHANKLNYYAREIENQANQIAENQKTISKYDEEINLMNIKINKKRTDLLKIETQLNLLNQQKESNSLLFKGTKNILENKAYFGKALKGTVADLIKVTTEYIPAIDSILANASQHLVVDKAETAVKAVNFLKDNNGGRATFIPLTSIQPKMIRDDHLLAIQGHPGFIDVASNLVTIDPQYEILNKFLLGNVIVASDIENANKISSILERRYMVVSLDGDTIRSGGVIIGGAKNTAQSLLGFDEKIKELENIVPGMRAAVQADESKINEIRNYRRSALELDSQLKQQITSLKTQKSSEQGLYDELQLKLQNITNKEFKVKEGVSASSPEASIEVLESQKIALESQFRAKNERVNTIAAEINSTLALKNDYEATRRKLTDAFSAKIGERERSKTLLEQSRERLASQYKMTFEAAEKVHHLEMNRDQAEEIVRQLREEIEGLGNVNLEALEQLVEEEKRYNEYAEGERELSAAKETLESAIADMDKIIITRLTNIINDVNDEFNNVFRTMFGGGTAKLFFSNPKDILESGVEIEAQPPGKNIKNLKLFSGGEKSLIAISLLFGILKARPLPLCILDEVEAALDEANVVRYAEYLQQLKEKTQFLVITHRHGTMSRVDTLFGATMQNRGVTSFFSIQLADAKKLVADEQKEELEPSNKNK", "aa_hexdigest": "3233d6bd1b66605abbdcf2f33b755637", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU3", "query_cov": 1.0, "subject_cov": 0.9300699300699301, "identity": 0.9990000000000001, "score": 1699.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_Q98PK8", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PK8" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "EFPKPENHBP_1254", "locus": "GCA000186005_01227" }, { "type": "cds", "sequence": "contig_1", "start": 672602, "stop": 672739, "strand": "-", "frame": 3, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687", "UniRef:UniRef90_UPI0024CCD326" ], "nt": "ATGAAACTCATTAAAGTTGAAGCCCACGGATTCAAATCATTTGCTGATCCTATTGTGCTTAAATTTGATGGTGGAGTAGCCGGAATTGTTGGCCCTAATGGTTCTGGTAAATCAAATATTAACGATGCTATCAAATGA", "aa": "MKLIKVEAHGFKSFADPIVLKFDGGVAGIVGPNGSGKSNINDAIK", "aa_hexdigest": "566a740d3cb0dc930db18b8fd124f59e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 672506, "stop": 672739, "observations": { "mutation": [ 672604 ], "stop": [ 672604 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024CCD326", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.9109999999999999, "score": 87.8, "evalue": 7.69e-22, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MKLVKLEAHGFKSFADPIVLRFDGGVAGIVGPNGSGKSNINDAIRWVLGEQSSKELRGDTMEDVIFAGSKTVQPMNKNRFL" }, "paralog": false, "identity": 0.8441558441558441, "score": 136.0, "evalue": 1.64e-43, "description": "Internal stop codon at 672604. Nonsense mutation around 672604.", "psc": { "uniref90_id": "UniRef90_UPI0024CCD326", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024CCD326", "UniRef:UniRef50_UPI0021DFD687" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687" ], "product": "AAA family ATPase" } }, "id": "EFPKPENHBP_1255", "locus": "GCA000186005_01228" }, { "type": "cds", "sequence": "contig_1", "start": 672837, "stop": 673412, "strand": "-", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_C4XDU4", "UniRef:UniRef90_C4XDU4" ], "nt": "GTGAGTGAAAGAGTTGGCAATTATCAAGATCTTGAATTTCTCGGAGACGCTATTTTACAGTATCTTTCGTCAGATTTTGTATTCAAAAAATTTCCAAATTTAAGTGCTGGTAATATGACTTTAATTCGTTCTAAATTAGTTTGCACAAAGTCACTAAATGAAATTAGCACTAAAATTGGATTAAAAGATTTTTTACTAACTGCACCTGGTCAAATGAGTAAAGATGCTAAACGCTCAGTTAAAGTTGGCGCTGACATTTTTGAAGCCTTTATTGCTGCTATTTATTTGGATACAAATCTAAAAGTAGTCAAAGAATTTTTAAACAAAACTGTTTTTGATATCAAAATTGGAGACTTTGACATCAAGCATCTTAAAGATGCTAAAACTGCTTTTCAAGAACAAATTCAATCATTCAGTAAACTAGCTGTTATGTATATAGTTAGTGAAAAAAGTCCAACTGAATTTGAAGCTCACGCAGTTCACGACAATAAAATTTATGGTATTGGTTTTGGTAAAAGCAAAGCTGAAGCCGAAGAAAATGCTGCACTAGATGCATTGCATAAAATGAAAAAATAA", "aa": "MSERVGNYQDLEFLGDAILQYLSSDFVFKKFPNLSAGNMTLIRSKLVCTKSLNEISTKIGLKDFLLTAPGQMSKDAKRSVKVGADIFEAFIAAIYLDTNLKVVKEFLNKTVFDIKIGDFDIKHLKDAKTAFQEQIQSFSKLAVMYIVSEKSPTEFEAHAVHDNKIYGIGFGKSKAEAEENAALDALHKMKK", "aa_hexdigest": "8e9a13a2f6ad83f7d183e08768bbcbaa", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU4", "query_cov": 1.0, "subject_cov": 0.809322033898305, "identity": 0.995, "score": 371.0, "evalue": 2.38e-129, "valid": true, "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_C4XDU4", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU4" ], "product": "Ribonuclease 3" }, "genes": [ "rnc" ], "id": "EFPKPENHBP_1256", "locus": "GCA000186005_01229" }, { "type": "cds", "sequence": "contig_1", "start": 674100, "stop": 674561, "strand": "-", "frame": 2, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "EC:2.3.1.274", "GO:0008610", "GO:0043811", "KEGG:K03621", "SO:0001217", "UniRef:UniRef50_Q98R48", "UniRef:UniRef90_C4XDU5" ], "nt": "ATGTACAAAATAGCATTTGATATTAATGGCAATGACAACGGCATTAATTCAGCTTTAGCAGCAACTAAATTATTTTTAAAAACCAACAAAAATTATGCAATTATTTTAGTTGGTCCTAAGGCTGAAATTGAAAAATATTATGGCAAAAACATGCCAGAAAATTTAGAAATAATTGACAACTCATCAGTGCCAAGCGATGTTAAAAACATTCGCCAATCTTTAAGAGAAAATACCTCAATGAATGTTGCACTTGATTTAGTAGCAAATAAAAAAGCAGATGCTGTACTTTCTTCAGGAGACTCTGGCACATATCTTGCAGCTGCTACTTTTAAAGTTAAAAGATTAGAAGGCATTTCAAGAGCAGCATTTATGCCCTTAATGCCTACAATCACAGGACGTAAATTCTTACTTCTTGATGTTGGAGCTAACTTAGAAACCACAAGTGATTATTTAGTTGAATGA", "aa": "MYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVE", "aa_hexdigest": "3c509b4348dba4600e103eb9c6b8ce5d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "plsX" ], "truncated": "5-prime", "pseudogene": { "start": 673797, "stop": 674618, "observations": { "mutation": [ 674102 ], "stop": [ 674102 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU5", "query_cov": 1.0, "subject_cov": 0.43714285714285717, "identity": 1.0, "score": 292.0, "evalue": 5.22e-97, "gene_start": 1, "gene_end": 153, "reference_start": 20, "reference_end": 172, "reference_sequence": "MVLNLTTTSLLVLNKIRSKMYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVEWTKIANSFDRVLLKNLAPRCALINIGTEDYKGTELIKEANNKLKEQHQLNYIGFQEPRDLLNGVCDVAVIDGYGGNLILKSLEGAILSFKNLLTSKIKLKPIRKLGYLLARGAFKDVAETLDYRNVGAAWIAGVNGVVIKSHGSSDEKAYYGALNQIKLALDNHILSEVKKDLNINEE" }, "paralog": false, "identity": 0.9963369963369964, "score": 525.0, "evalue": 3.48e-191, "description": "Internal stop codon at 674102. Nonsense mutation around 674102.", "psc": { "uniref90_id": "UniRef90_C4XDU5", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q98R48", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU5", "EC:2.3.1.274", "UniRef:UniRef50_Q98R48", "KEGG:K03621", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R48" ], "product": "Phosphate acyltransferase" } }, "id": "EFPKPENHBP_1257", "locus": "GCA000186005_01230" }, { "type": "cds", "sequence": "contig_1", "start": 674575, "stop": 676200, "strand": "-", "frame": 1, "gene": "dhaL", "product": "DhaL domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354744.1", "SO:0001217", "UniParc:UPI0001A8C31B", "UniRef:UniRef100_C4XDU6", "UniRef:UniRef50_A0A449B6P5", "UniRef:UniRef90_C4XDU6" ], "nt": "ATGACTAAGATTTTAGACGGTAAGCTTTTTTGTCAACTTTTTATTTCCGGAGCTAATAACATTATTAACTCTAAAAATAAGATCGACGCTTTAAATGTTTTTCCTGTTCCAGATGGCGACACAGGAACTAACATGTCATCAACAGCTCAAGCTGCTGCTGATGCTTTGGCTTTAAATAACTCAAATCACTTAGGTGAAGTAACTAACACTATTTCAAGAAATATGCTTTTAGGAGCTCGCGGAAACTCAGGTGTTATTTTAAGCCAAATTTTTAAAGGTTTTGCAATTGCGTTTAAAGAAGTTGAAAGCGTTGACATTAAAGGACTTTTAGAAGGATTTAAGCAAGCGACTAAAAAAGCTTATTCATCAGTTTTAAAACCTGTTGAAGGTACCATTTTAACAGTTATTAGAGAAACAACAGAAGCATTAGAAAAAGAAGTAAATAATAAAACTTCACTAGAAGAATTTTTCAAATTAGCTAAAAATGCAGCTCGTAAAGCATGTGACAATACTCCTAACAAATTAAAGATCTTACGTGAAGTTGGCGTAACTGACTCAGGTGGTGAAGGATTATATACAATTATTAGTGGTATGGAAGCAGCATTAAATGCTGAAGCCATTAAAATTAGTGAATCAGAAGAAAACATTGATACTTTTATCAGCTCAAAAGAAGTTTATGATGGCGAATTTGGTTATTGTACTGAATTCATTATTGAATTAAATGACGAAAAAGACTTTGATAAAGCTAATTTTGAAAAATCATTACTTAAAAAAGCTAATTCTTTAGTTGTAGTTCAAGATGAAAAAATTGTTAAAGTTCATGGTCATACATTAAAACCTGGTGATATGTTAAACTTTGGTCAAAAATATGGTGAATTTATTAAAATAAAGTCAGAAAATATGACTCGTCAAGCAAGTGAAAGTCGTAATAAAAACGTTATTATTAACCAAAATGATGAACAATCAAACAAAAAATGTGCCATTATTTCATGTAACTTAGGCAGTGGAATTATTGCAAGAATGAAAGAATTTGGAGTTGACGCTATTATTGAAAGTGGTCAGACACAAAACCCTTCAGCTCAAGATATTATTGATGCAATTAACTCAGTAAATAGCAAAAACGTTTTCATTTTACCTAATAACTCAAACATTTTCCTAGCAGCTGAACAAGCAGCTCAAGCTATTACAAATAAAAAGGTATTTATTATTCAAACTAGAAGTCAAATTCAAGGATTAACAGCTTTAATGAACTTTAATTCTGAATCAAGTCCTAAAGAAAACCGTGAAACAATGTCTGAATCTATTAAGCTTGTAACCACAGCTGAAGTAACCCAAGCAATTAGAAGCACAAAAATTGACAATGTTAAAATCAAAGAAGGTCAATTCATTGGCATTACTGATGGCAAGATTTTAGTTAGTGAAAATAACTACATTGATGCAGCTAAAAAATTGATTAAAAAATCAATTAACAGTGATCATGAACTTGTTACAGTTTACTATGGAAATGATGCAAGTGAAGTTGATGCTAACGAAATTAAAAACTTTTTAGAAAGCCATTATGATGTTGAAGTTGAAGTAGTGAATGGTGCTCAACCTAACTACCACTTCATTATTGGTTTTGAATAA", "aa": "MTKILDGKLFCQLFISGANNIINSKNKIDALNVFPVPDGDTGTNMSSTAQAAADALALNNSNHLGEVTNTISRNMLLGARGNSGVILSQIFKGFAIAFKEVESVDIKGLLEGFKQATKKAYSSVLKPVEGTILTVIRETTEALEKEVNNKTSLEEFFKLAKNAARKACDNTPNKLKILREVGVTDSGGEGLYTIISGMEAALNAEAIKISESEENIDTFISSKEVYDGEFGYCTEFIIELNDEKDFDKANFEKSLLKKANSLVVVQDEKIVKVHGHTLKPGDMLNFGQKYGEFIKIKSENMTRQASESRNKNVIINQNDEQSNKKCAIISCNLGSGIIARMKEFGVDAIIESGQTQNPSAQDIIDAINSVNSKNVFILPNNSNIFLAAEQAAQAITNKKVFIIQTRSQIQGLTALMNFNSESSPKENRETMSESIKLVTTAEVTQAIRSTKIDNVKIKEGQFIGITDGKILVSENNYIDAAKKLIKKSINSDHELVTVYYGNDASEVDANEIKNFLESHYDVEVEVVNGAQPNYHFIIGFE", "aa_hexdigest": "daf6d8778c82e3384ca53ddab710e206", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C31B", "ncbi_nrp_id": "WP_013354744.1", "uniref100_id": "UniRef100_C4XDU6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31B", "RefSeq:WP_013354744.1", "UniRef:UniRef100_C4XDU6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU6", "uniref90_id": "UniRef90_C4XDU6" }, "psc": { "uniref90_id": "UniRef90_C4XDU6", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_A0A449B6P5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6P5" ], "product": "Dihydroxyacetone (Glycerone) kinase, DhaK2 subunit" }, "genes": [ "dhaL" ], "id": "EFPKPENHBP_1258", "locus": "GCA000186005_01231" }, { "type": "cds", "sequence": "contig_1", "start": 676193, "stop": 676483, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354745.1", "SO:0001217", "UniParc:UPI0001A8C31C", "UniRef:UniRef100_C4XDU7", "UniRef:UniRef50_C4XDU7", "UniRef:UniRef90_C4XDU7" ], "nt": "ATGACTTTAGAAGAAATTAGAAAAGTTTTTTTGAATATTCTTGAATCAACGCCGGGGGTTTATGGAATCTGTGAAAATGCAGAACAATGTCCTAATTTAGGCCAAGAATATTTAAGTATTCAAAATATAAACAATGAATGGCATTTCAGCGCTACAATTATTATTTTAAAATATGCTAATGCAAAAAATGTTGTTAACTCAATTAGTTCTCACTTAAGATACGAACTCAATAAGAAAAAGGAACATTTAGGCAAATTAAATGTATTTATAGGAGGTCTTGCAAATGACTAA", "aa": "MTLEEIRKVFLNILESTPGVYGICENAEQCPNLGQEYLSIQNINNEWHFSATIIILKYANAKNVVNSISSHLRYELNKKKEHLGKLNVFIGGLAND", "aa_hexdigest": "3a51432363c8cd1ca400ef9de6aa183e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C31C", "ncbi_nrp_id": "WP_013354745.1", "uniref100_id": "UniRef100_C4XDU7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31C", "RefSeq:WP_013354745.1", "UniRef:UniRef100_C4XDU7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU7", "uniref90_id": "UniRef90_C4XDU7" }, "psc": { "uniref90_id": "UniRef90_C4XDU7", "uniref50_id": "UniRef50_C4XDU7" }, "pscc": { "uniref50_id": "UniRef50_C4XDU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10877.31659999999, "isoelectric_point": 6.275900077819824 }, "id": "EFPKPENHBP_1259", "locus": "GCA000186005_01232" }, { "type": "cds", "sequence": "contig_1", "start": 676617, "stop": 677159, "strand": "+", "frame": 3, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_C4XDU8", "UniRef:UniRef90_C4XDU8" ], "nt": "ATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGA", "aa": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQR", "aa_hexdigest": "bfc34cb513bf9df5df8f4a04af468ad4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "recO" ], "truncated": "3-prime", "pseudogene": { "start": 676617, "stop": 677297, "observations": { "mutation": [ 677157 ], "stop": [ 677157 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU8", "query_cov": 1.0, "subject_cov": 0.7964601769911505, "identity": 1.0, "score": 362.0, "evalue": 3.42e-126, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQRWTKELKSIYYMYNDLKIFLQICISKVNQTIFYELNKYLEENGIYLN" }, "paralog": false, "identity": 0.995575221238938, "score": 455.0, "evalue": 3.8e-165, "description": "Internal stop codon at 677157. Nonsense mutation around 677157.", "psc": { "uniref90_id": "UniRef90_C4XDU8", "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_C4XDU8", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU8", "UniRef:UniRef50_C4XDU8", "KEGG:K03584", "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU8" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" } }, "id": "EFPKPENHBP_1260", "locus": "GCA000186005_01233" }, { "type": "cds", "sequence": "contig_1", "start": 677480, "stop": 678082, "strand": "+", "frame": 2, "gene": null, "product": "Nitroreductase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9", "UniRef:UniRef90_C4XDU9" ], "nt": "ATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGA", "aa": "MSFIENNLKRGSTRDYQIGYEISEEDKEKLIKTIQNAPTSNNYFASSVIVIEDQNTKDKLAEILQQKQVRECSMFFIFLADNNRIDYVLKTKNKNIENKTLNSLLLSVGDAFIQATMLQDAATELDLGTCFIGGVRGHISEINRLLKIKNSAFPAVALTVGKAAKKADLKPKINRIYYEQYSYAQVVDEIVPYNEKLTNY", "aa_hexdigest": "ba2d98a50dfbad4f7a08c7e2fc3e9f55", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XDU9", "query_cov": 1.0, "subject_cov": 0.8403361344537815, "identity": 1.0, "score": 386.0, "evalue": 4.21e-135, "valid": true, "product": "Nitroreductase domain-containing protein", "uniref50_id": "UniRef50_C4XDU9" }, "pscc": { "uniref50_id": "UniRef50_C4XDU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9" ], "product": "Nitroreductase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1261", "locus": "GCA000186005_01234" }, { "type": "cds", "sequence": "contig_1", "start": 678265, "stop": 678408, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGA", "aa": "MAKFKRVFMIVTDGLGIGPDKDQRAFGDKGANTILSASKSSMFFIDT", "aa_hexdigest": "073303c3d294ddbd4feec3b74c7ef7c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5113.885799999999, "isoelectric_point": 9.397443580627442 }, "id": "EFPKPENHBP_1262", "locus": "GCA000186005_01235" }, { "type": "cds", "sequence": "contig_1", "start": 678535, "stop": 678768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGA", "aa": "MMGIKTMVPFPTFTENGFPQDLLDELSKAFDGRKIICNKSGSGTEMIDEYAEQQKKDGSIIVYTSMDSVLQIAAHEE", "aa_hexdigest": "6b70b7a38edd571932330ad7b6fe5ca5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8577.6579, "isoelectric_point": 4.5660696029663095 }, "id": "EFPKPENHBP_1263", "locus": "GCA000186005_01236" }, { "type": "cds", "sequence": "contig_1", "start": 678934, "stop": 679398, "strand": "+", "frame": 1, "gene": null, "product": "Phosphopentomutase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C5TNN4" ], "nt": "ATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACTTGACTCAAAATTAGGTAAATTAATTAATGCTATGAAAGAAGACGACTTATTAATTATAACAAGTGACCACGGTAATGATCCTCTTTATCCAGGATTCAACCACACTCGTGAATTACTTCCTTTAACAATCTTTTCAAAAAGATTCAAAAAACCTAAAGTTCTTGAAGATGTTCTTGGTTTAGGAACAAGTGGAAATATTGTTGCCCGTAACTGA", "aa": "MILNELQKAGVNVIGIGKIFDIFVGQGISESLHSDGDAHGMDLTIKCAEERPENTFVFTNLVQFDSHYGHRRDVDGYASNIALLDSKLGKLINAMKEDDLLIITSDHGNDPLYPGFNHTRELLPLTIFSKRFKKPKVLEDVLGLGTSGNIVARN", "aa_hexdigest": "cd97e1534fa4d8b09c15c4b3354eb5a3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1C5TNN4", "query_cov": 0.8961038961038961, "subject_cov": 0.8518518518518519, "identity": 0.5, "score": 146.0, "evalue": 4.76e-42, "valid": false, "product": "Phosphopentomutase", "ec_ids": [ "5.4.2.7" ], "uniref50_id": "UniRef50_A0A1C5TNN4", "kegg_orthology_id": "K01839" }, "pscc": { "uniref50_id": "UniRef50_A0A1C5TNN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C5TNN4" ], "product": "Phosphopentomutase" }, "genes": [], "id": "EFPKPENHBP_1264", "locus": "GCA000186005_01237" }, { "type": "cds", "sequence": "contig_1", "start": 679540, "stop": 680328, "strand": "+", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "GO:0003677", "GO:0043937", "SO:0001217", "UniRef:UniRef50_C4XDV1", "UniRef:UniRef90_C4XDV1" ], "nt": "ATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTAAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGA", "aa": "MNFTKFVKNEIINRKKNEVESLEYLRGLIFGNGLIKNDSIHLNIRDEEFFYLIIELIKKVNLDYQIKNSKTIVLNKKDIDLDVKFLKPSLFFGGVFQTGGSISNLGKTSYHLQLSSNYENFIDIIMSKLNEYYFNFQKLKHQNKYIIYVKQKEKIEDFLKAILALESFYKFTDITIKRDFENSFNRINNIDIYNIKKAVEANVKHIENLNYIFENKLENKFKDEQILLYKTVLNNPEAPLSNIILSLEKEHNLIKSKSTIHH", "aa_hexdigest": "4079e155a7f43b90c638e0046b8a8881", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDV1", "query_cov": 1.0, "subject_cov": 0.9390681003584229, "identity": 1.0, "score": 507.0, "evalue": 4.52e-181, "valid": true, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_C4XDV1", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV1" ] }, "genes": [ "whiA" ], "id": "EFPKPENHBP_1265", "locus": "GCA000186005_01238" }, { "type": "cds", "sequence": "contig_1", "start": 680379, "stop": 680720, "strand": "+", "frame": 3, "gene": null, "product": "DUF86 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526970.1", "SO:0001217", "UniParc:UPI0001F59AB8", "UniRef:UniRef100_C4XDV2", "UniRef:UniRef50_C4XDV2", "UniRef:UniRef90_C4XDV2" ], "nt": "ATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATTAA", "aa": "MKERMKYLNIKIQEKMNHIHYLTGTVVHSVQKLEYRLTYLITLKKTIDEYEKKGKFKHNLFLKVVLEASSEAQKIFRSLYENTFGNLSQKAYQNGILSKNEHLELSKVVEDRN", "aa_hexdigest": "a4db8ce7076eae27f11e187070c02f6f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59AB8", "ncbi_nrp_id": "WP_013526970.1", "uniref100_id": "UniRef100_C4XDV2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59AB8", "RefSeq:WP_013526970.1", "UniRef:UniRef100_C4XDV2" ] }, "ips": { "uniref100_id": "UniRef100_C4XDV2", "uniref90_id": "UniRef90_C4XDV2" }, "psc": { "uniref90_id": "UniRef90_C4XDV2", "product": "DUF86 domain-containing protein", "uniref50_id": "UniRef50_C4XDV2" }, "pscc": { "uniref50_id": "UniRef50_C4XDV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV2" ], "product": "DUF86 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1266", "locus": "GCA000186005_01239" }, { "type": "cds", "sequence": "contig_1", "start": 680929, "stop": 681894, "strand": "+", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_013526971.1", "SO:0001217", "UniParc:UPI0001A8C322", "UniRef:UniRef100_C4XDV3", "UniRef:UniRef50_A0A3P8KAF3", "UniRef:UniRef90_C4XDV3" ], "nt": "ATGAATTTTATTTATGGCTCAGAAAATTTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAA", "aa": "MNFIYGSENFFIEQEINKIVKKFPNSAITTFNIENDENVDSLIQFISSGDLFNKKRIIVINDLFYFEKKLSSNDETNVNNLIKSIDNNSSDELIFVNTNISNKEQINKNFFTNFIFNKLQQNNFIEANSIDDNQLTKKVVSLVQQKGGTIDALATSALLKKIPNDLYLINLEIDKLINQNKHITENMINVSVIDIYVEDAFGFSNSFETNDFNLIWKKYKEKLIEGVDVTVLIAQTSQLFILANQIYAYKNIGKTLENLASDFKINQYRVKKVSYLLTRLGTLKIRRMIKSLAKLDQDIKEGKTDPTIGFERFLIKFFING", "aa_hexdigest": "75553d84ac2538df7f415568daa5558a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C322", "ncbi_nrp_id": "WP_013526971.1", "uniref100_id": "UniRef100_C4XDV3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C322", "RefSeq:WP_013526971.1", "UniRef:UniRef100_C4XDV3" ] }, "ips": { "uniref100_id": "UniRef100_C4XDV3", "uniref90_id": "UniRef90_C4XDV3" }, "psc": { "uniref90_id": "UniRef90_C4XDV3", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A3P8KAF3", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KAF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KAF3" ], "product": "DNA-directed DNA polymerase" }, "genes": [ "holA" ], "id": "EFPKPENHBP_1267", "locus": "GCA000186005_01240" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 682644, "stop": 682916, "strand": "+", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 209.8, "evalue": 2.6e-25, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "AGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAAT", "id": "EFPKPENHBP_43", "locus": "GCA000186005_01241" }, { "type": "cds", "sequence": "contig_1", "start": 683008, "stop": 683130, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354753.1", "SO:0001217", "UniParc:UPI0001E32EB1", "UniRef:UniRef100_A0AB32XC49", "UniRef:UniRef50_A0AB32XC49", "UniRef:UniRef90_A0AB32XC49" ], "nt": "ATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAA", "aa": "MSVIDKIKELIKENNGYMSTAILDKNKISRNYLKFLIEKN", "aa_hexdigest": "180a7bcc61b14991a91355f416da1c11", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32EB1", "ncbi_nrp_id": "WP_013354753.1", "uniref100_id": "UniRef100_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EB1", "RefSeq:WP_013354753.1", "UniRef:UniRef100_A0AB32XC49" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC49", "uniref90_id": "UniRef90_A0AB32XC49" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC49", "uniref50_id": "UniRef50_A0AB32XC49" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC49" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4716.521099999999, "isoelectric_point": 9.456109809875489 }, "id": "EFPKPENHBP_1268", "locus": "GCA000186005_01242" }, { "type": "cds", "sequence": "contig_1", "start": 683387, "stop": 683587, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6", "UniRef:UniRef90_C4XDV6" ], "nt": "ATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAA", "aa": "MFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL", "aa_hexdigest": "9db485b1af58dc8b45d309d01aa42a9d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 683153, "stop": 683587, "observations": { "mutation": [ 683165, 683177, 683180, 683225, 683351 ], "stop": [ 683165, 683177, 683180, 683225, 683351 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDV6", "query_cov": 1.0, "subject_cov": 0.4583333333333333, "identity": 1.0, "score": 135.0, "evalue": 1.07e-39, "gene_start": 1, "gene_end": 66, "reference_start": 79, "reference_end": 144, "reference_sequence": "MCRYWYCRWWTLCISIEIQKVHFFWISAMFLQKITNVLYHRTEANFSTNYNTSKIDKKIKCHRIIDWKLNLGVEKVKIMFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL" }, "paralog": false, "identity": 0.9583333333333334, "score": 276.0, "evalue": 2.33e-97, "description": "Internal stop codon at 683165, 683177, 683180, 683225, 683351. Nonsense mutation around 683165, 683177, 683180, 683225, 683351.", "psc": { "uniref90_id": "UniRef90_C4XDV6", "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDV6", "UniRef:UniRef50_C4XDV6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7998.4307999999955, "isoelectric_point": 9.329042625427245 }, "id": "EFPKPENHBP_1269", "locus": "GCA000186005_01243" }, { "type": "cds", "sequence": "contig_1", "start": 683739, "stop": 684626, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8", "UniRef:UniRef90_C4XDV7" ], "nt": "ATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTKYQVEASNYAFTTIEPNISSVPLKDPRLYELAKIVKPGRIVPATFDFVDIAGLVKGASKGEGLGNKFLGNIREVDAVVHVVRCFDDKNIMHVANEVNPVNDKDVINMELMLADLETINNIINRIHKKAKSGDKEAILEENLALKIKDLLENNKPARMIKNLSEDEEKLIKNYHLLTSKPMIYVANMSAEEIAEYENAPLYKQLKVSVEDDVKILPICVQLESEISQMDESESKEWLDSYNIKFSGLDLLAREAFDLLKLKTYFTAGEMEVKA", "aa_hexdigest": "bb607f6fba9700308db2b1169e322269", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XDV7", "query_cov": 1.0, "subject_cov": 0.8060109289617486, "identity": 1.0, "score": 565.0, "evalue": 4.06e-202, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_A0A3D5Z7W8", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D5Z7W8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "EFPKPENHBP_1270", "locus": "GCA000186005_01244" }, { "type": "cds", "sequence": "contig_1", "start": 684642, "stop": 684839, "strand": "+", "frame": 3, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "nt": "ATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAA", "aa": "MLAPACAGIIHSDFEKKFIKADVISYQDYIDNNGELGARNAGKIRSEGKNYVMQDGDICHFKFGK", "aa_hexdigest": "332c967efb390a89ade3681ad845c738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378MX97", "query_cov": 0.9384615384615385, "subject_cov": 0.8591549295774648, "identity": 0.639, "score": 92.0, "evalue": 2.89e-23, "valid": false, "product": "GTP-dependent nucleic acid-binding protein engD", "uniref50_id": "UniRef50_A0A0P9MY34" }, "pscc": { "uniref50_id": "UniRef50_A0A0P9MY34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "EFPKPENHBP_1271", "locus": "GCA000186005_01245" }, { "type": "tRNA", "sequence": "contig_1", "start": 684851, "stop": 684924, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 65.6, "nt": "AGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 684883, 684885 ], "id": "EFPKPENHBP_13", "locus": "GCA000186005_01246" }, { "type": "cds", "sequence": "contig_1", "start": 685375, "stop": 685485, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACCTTTTACCTTATTTATTATTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGA", "aa": "MFGPFTLFIILIVAAILLKIEIKENTRFATRVTGYR", "aa_hexdigest": "d709db95fa6de6d389cb775ec6b87363", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4157.017100000002, "isoelectric_point": 10.266412925720214 }, "id": "EFPKPENHBP_1272", "locus": "GCA000186005_01247" }, { "type": "cds", "sequence": "contig_1", "start": 686232, "stop": 686549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTGATGGTGTTAATGATGCTCCCGCTCTTAAAACTAGTGACATTGGTTGTGCTATGGGAATTACAGGAACTGAAGTTTCAAAACAAGCAGCTGATTTAATCTTAGTTGACGATAACTTCAATACTATTGTTAATTCAGTAAGAAATGGTCGTTTAATTTTTGACAAAATCAAAACAGTGATACTTAACTTATTAATTAGCTCACTAACAGAAATTTTAGTAATGCTTATTGGTATGGTCGCTTTCTTCTTAGCATATAAAGATTATTATCCAGATGGATTTTACATTTTTGGAGCTAGCCAATTATTATGA", "aa": "MTGDGVNDAPALKTSDIGCAMGITGTEVSKQAADLILVDDNFNTIVNSVRNGRLIFDKIKTVILNLLISSLTEILVMLIGMVAFFLAYKDYYPDGFYIFGASQLL", "aa_hexdigest": "4bc9ea45c1223c7fa7907418ac6d2104", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11385.136099999992, "isoelectric_point": 4.392767524719238 }, "id": "EFPKPENHBP_1273", "locus": "GCA000186005_01248" }, { "type": "cds", "sequence": "contig_1", "start": 686574, "stop": 686840, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGATTGATCCACCTCGGGCAGAGGTTGCAAATTCAATTAGAGAAGCTCAAGGTGCTGGTATTAAAGTTGTTATGATCACAGGTGATCAAGTAACTACAGCAAGAGCTATAGCTCAAAATTTAGGCATTTATCAAGAAAGCGATACTTGTCTTGAAGGCAAAGATTTAGCCAAAATGAGTGAAGAAGAATTAAGAGCTAAAGTTGAACATATTTCAGTTTATGCTCGTGTTAATCCTGAAGACAAATTAAGAATTGTTCAAGCATGA", "aa": "MIDPPRAEVANSIREAQGAGIKVVMITGDQVTTARAIAQNLGIYQESDTCLEGKDLAKMSEEELRAKVEHISVYARVNPEDKLRIVQA", "aa_hexdigest": "0a8369a165fe0e7449d5ec3d92cfbd6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 85, "aa_cov": 0.9431818181818182, "hmm_cov": 0.8117647058823529, "evalue": 8.547214931597635e-09, "score": 32.37348937988281, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9651.904999999993, "isoelectric_point": 5.089272499084472 }, "id": "EFPKPENHBP_1274", "locus": "GCA000186005_01249" }, { "type": "cds", "sequence": "contig_1", "start": 686946, "stop": 687095, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTCGATTCAAATCGTAAAATGCACTCTGTAGTATTCAAAGAAAAAGATAGAATGCTTTTAGTTACTAAAGGTGCTCCTGATGTTTTATTAAAAAGATGTAATAATTTAACTATTGATGATATTAAAGAAATTAATCACCAATGA", "aa": "MPFDSNRKMHSVVFKEKDRMLLVTKGAPDVLLKRCNNLTIDDIKEINHQ", "aa_hexdigest": "5574b6ef4dc0134333c5777e7845c54c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5739.6747, "isoelectric_point": 8.978850364685059 }, "id": "EFPKPENHBP_1275", "locus": "GCA000186005_01250" }, { "type": "cds", "sequence": "contig_1", "start": 687294, "stop": 687437, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAACAAAAAGGGTTGGTTAAAAACCTTTTAGCAGTTGAAACCCTAGGTTCTGCTTCAATTATTTGTAGTGATAAAACAGGAACACTAACAGAAAATAAAATGACAGTAGTTGATGCTTATCGTTTTAGAACTCTTTGA", "aa": "MSKQKGLVKNLLAVETLGSASIICSDKTGTLTENKMTVVDAYRFRTL", "aa_hexdigest": "7cff828fbd3b514d5e27dff5fb1ad487", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5132.972300000001, "isoelectric_point": 9.428839683532715 }, "id": "EFPKPENHBP_1276", "locus": "GCA000186005_01251" }, { "type": "cds", "sequence": "contig_1", "start": 687636, "stop": 688382, "strand": "-", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "nt": "ATGAACAAAAAAGACTATTCCAATTATCAAGGCTTGACTTCAAAAGAAGTTGAAGCAAACCTTGCAAAGTATGGTGAAAACGCTTTAATTAAAAAGAAAAAACTAAATCCAATAGTTGCTTTCTTTAGACAATTTGTTGATCCAATGGTTATTCTTTTAATCATAGCGGCAGCAATTAGTTTAACTTTGGCAATTGTTGAACATTTTCAAGGCGGAAAAAGCACAACCGATTTAATTATCTCTTATGTTGAACCTGGAATTATTATGCTAGTTATTATTTTAAATAGTATGTTAGGAGCTTATCAAGAGGTCAAAAGTGATCAAGCGGTGCGAGCCCTTGAAAAAGTTAATCAGGTCAATGCTACAGTAATTAGGGATGGCAAAGTCCAAATTATTCCCGCTAATTTATTAGTGCCTGGTGACTTAGTTGTTTTAGCTGCAGGAGATACAATTAATGCTGATGGTCGCTTAATTGAAGCTTCAAACTTAGAAGTTGTAGAAGCTTCATTAACTGGTGAAAGTTTACCAGTAAGTAAAGTCGCAAATTTAAATGCAACTGATGACAAAATCTTAGCTAACAACAAACACTTAGTTTACTCAGGCACTTATGTAATTAATGGACGGGCAACTTTTGTAGTTGAACATACAGCTAGTGAAACTGAAATTGGCAAAATTAATTCAATGATTCAAAAACAAAATAAAAATGCAACACCACTACAAATCAAACTTAATAAATTAAGCAAATGA", "aa": "MNKKDYSNYQGLTSKEVEANLAKYGENALIKKKKLNPIVAFFRQFVDPMVILLIIAAAISLTLAIVEHFQGGKSTTDLIISYVEPGIIMLVIILNSMLGAYQEVKSDQAVRALEKVNQVNATVIRDGKVQIIPANLLVPGDLVVLAAGDTINADGRLIEASNLEVVEASLTGESLPVSKVANLNATDDKILANNKHLVYSGTYVINGRATFVVEHTASETEIGKINSMIQKQNKNATPLQIKLNKLSK", "aa_hexdigest": "4e78b4d62684d3dfb594b35b06e5267a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FFE00D", "query_cov": 1.0120967741935485, "subject_cov": 0.8869257950530035, "identity": 0.502, "score": 222.0, "evalue": 9.12e-69, "valid": false, "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI002FF3AF86" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF3AF86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "EFPKPENHBP_1277", "locus": "GCA000186005_01252" }, { "type": "tRNA", "sequence": "contig_1", "start": 688814, "stop": 688905, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 73.9, "nt": "GGAACAGTACTCAAGTCGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGGCAACACCCGCGGAGGTTCGAATCCTCTCTGTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 688868, 688870 ], "id": "EFPKPENHBP_31", "locus": "GCA000186005_01253" }, { "type": "cds", "sequence": "contig_1", "start": 689104, "stop": 689364, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGATACCAACATAAAACTAGGAAATCAAGCAACTGCTTTTAGTGAAATACTAAAAGATCAATATTATAAAGCACTGTTAACTGACTCAAAACAACACAAGAGTTCATTGAGTAATAGTACATGGGGAAAATATTCTGAACCTTATGACAAGATTTTTACTAGAACCAATTTAAAATATGAAAACGCAAACTTCTTTCCTTTATACGATATTTTTAAAGATAATATTCTCAAAGATTATCAAACAATAGAAGAATGA", "aa": "MGDTNIKLGNQATAFSEILKDQYYKALLTDSKQHKSSLSNSTWGKYSEPYDKIFTRTNLKYENANFFPLYDIFKDNILKDYQTIEE", "aa_hexdigest": "d6af4ff35a32557b368bc92d5ce06ce7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10102.138099999993, "isoelectric_point": 5.775148582458496 }, "id": "EFPKPENHBP_1278", "locus": "GCA000186005_01254" }, { "type": "cds", "sequence": "contig_1", "start": 689404, "stop": 689577, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTAAATTTAAAACTTTAGGACCAATTCAAAATAATTTCACTTTTGTTATAGATCATTTAGATAGTCCAGGAGCAGCAAAAAGTGGCGAATCAAAATCAAGTTTATTAAGTAAGCAAGGCGCTCAAGAAGCTGATGAAGCTTACAACTTAAAGAACCTTATGGATTGA", "aa": "MGVKFKTLGPIQNNFTFVIDHLDSPGAAKSGESKSSLLSKQGAQEADEAYNLKNLMD", "aa_hexdigest": "5532db168bd029edaecbddbe88526eef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6142.8354, "isoelectric_point": 5.641122245788575 }, "id": "EFPKPENHBP_1279", "locus": "GCA000186005_01255" }, { "type": "cds", "sequence": "contig_1", "start": 689779, "stop": 689952, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGAATATTTTAAATTTATATGAAACATCTGAAGAATACAAAAAATATGGTTTAGCTAGTGTTATTGAACATTTAAATCTTGATGTTATTGGGCTTATTGAAAATAAAACAAAAGCTTCAGCACAAAAATTTTGTGAATACTTAAAAAAATTTACGGGAAACAACAATTGA", "aa": "MWNILNLYETSEEYKKYGLASVIEHLNLDVIGLIENKTKASAQKFCEYLKKFTGNNN", "aa_hexdigest": "f427d57dde27cf0904ea9bfeb67a583a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6628.495500000001, "isoelectric_point": 6.543952751159668 }, "id": "EFPKPENHBP_1280", "locus": "GCA000186005_01256" }, { "type": "cds", "sequence": "contig_1", "start": 690754, "stop": 690921, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAACAATTTATTAAATTATTGGTAGGGTCTTTTTCTTTAATATCCTTACCTTTTTTATCAACTCAATGTAGCTTTTTTGAAATGAATAAAACACAAGAAAAAAACAAAGATGAAATTCAAAATGACTTAAATAATGCGGTTGAAAAAATCAAAATTTGA", "aa": "MKKKQFIKLLVGSFSLISLPFLSTQCSFFEMNKTQEKNKDEIQNDLNNAVEKIKI", "aa_hexdigest": "1d662c39a195fe31e3711c808814489d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6408.464800000002, "isoelectric_point": 9.252131843566893 }, "id": "EFPKPENHBP_1281", "locus": "GCA000186005_01257" }, { "type": "cds", "sequence": "contig_1", "start": 691440, "stop": 692267, "strand": "-", "frame": 2, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80", "UniRef:UniRef90_C4XDW2" ], "nt": "ATGACAAAAATTATTCATAAAGAATTAGTGCCTATTCTTGATACACCTGTTATTGATAGATTAGTTAATGAAGCACTTGATTCAGGTATTGAGGAAATTTTAATTGTTTTAAGTGAAAGGAAAAAGGATTTATTAAAATTTTTCTCAGTTGATCAAGATTTACAAACAGAATTAATTATCAAAGGTAAAAAAGATATTTTAGAAAAAGTTAAAAGAACAAATCGCTCTTCATGTATTACTAGAGTAATTCAAAAGGAACAATTTGGTTTAGGTCATGCACTTGCAAGTTGTAAAGAATATATTGACAATGAGCCGTTTGCTATTATTTTAGGTGATGACTTAATTAAATCAAAAACTCCTGCTATTAAGCAATTAATCGAGTTTTATAATAAAACTGGAGCAAATGTTATTGGTGTACAATCAGTAGCAAAAGAATATATTCATAAATATGGAATTGTTAATCCAATAAACTTGAAAGATAAAGATAAGAAATATTTTGAAATTAATGGTGCAGTTGAAAAACCTAAGGCTGATAAAGCACCATCAAATAAAGCTATACTTGGACGTTATGTCTTTAATCCAGAAATTTTAGATATCTTATCTAGAATTGAATATGATGGCAAAAATGAAATTCAAGCAGTTGATGCATTTGATGAATTAATGAAAAAATATAAACAAAAAATTTATGCCTATGAATTTGAAGGCATCAGATATGATCTTGGATCAATGGAAGGCTTTGTTAAAGCAAACATTGATTATGCTTTAGAAGAACCTGAAATTGCAGACAAGATTAAAAGTTTTATTAATGAAAAAGTTAAAGAATTTTAA", "aa": "MTKIIHKELVPILDTPVIDRLVNEALDSGIEEILIVLSERKKDLLKFFSVDQDLQTELIIKGKKDILEKVKRTNRSSCITRVIQKEQFGLGHALASCKEYIDNEPFAIILGDDLIKSKTPAIKQLIEFYNKTGANVIGVQSVAKEYIHKYGIVNPINLKDKDKKYFEINGAVEKPKADKAPSNKAILGRYVFNPEILDILSRIEYDGKNEIQAVDAFDELMKKYKQKIYAYEFEGIRYDLGSMEGFVKANIDYALEEPEIADKIKSFINEKVKEF", "aa_hexdigest": "35ea3657a8e612087c2d43f66493c65d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_C4XDW2", "query_cov": 1.0, "subject_cov": 0.9105960264900662, "identity": 1.0, "score": 533.0, "evalue": 7.68e-191, "valid": true, "product": "UTP--glucose-1-phosphate uridylyltransferase", "uniref50_id": "UniRef50_W5UT80" }, "pscc": { "uniref50_id": "UniRef50_W5UT80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "EFPKPENHBP_1282", "locus": "GCA000186005_01258" }, { "type": "tRNA", "sequence": "contig_1", "start": 692465, "stop": 692540, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 65.7, "nt": "GATCTTATAGCTCAATGGACAGAGTGTTGGGTTTCGGCCCCAAAGGTTAAGGGTTCGAGTCCCTTTAAGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 692505, 692507 ], "id": "EFPKPENHBP_30", "locus": "GCA000186005_01259" }, { "type": "tRNA", "sequence": "contig_1", "start": 693559, "stop": 693634, "strand": "-", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 79.1, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCGCTGGTTCGATTCCTGCCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 693599, 693601 ], "id": "EFPKPENHBP_29", "locus": "GCA000186005_01260" }, { "type": "tRNA", "sequence": "contig_1", "start": 693639, "stop": 693715, "strand": "-", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 75.8, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGTCTCCCTGTCACGGAGAAGATCGCGGGTTCAATTCCCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 693712, 693714 ], "id": "EFPKPENHBP_28", "locus": "GCA000186005_01261" }, { "type": "tRNA", "sequence": "contig_1", "start": 693719, "stop": 693795, "strand": "-", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 72.3, "nt": "CGCGGAGTGGAGAAGTTTGGTATCTCGCGAGGCTCATAACCTCGAGGCCGCTGGTTCGAGTCCAGCCTCCGCCACCA", "db_xrefs": [ "SO:0000266" ], "id": "EFPKPENHBP_27", "locus": "GCA000186005_01262" }, { "type": "tRNA", "sequence": "contig_1", "start": 693835, "stop": 693927, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 76.1, "nt": "GGGTGATTACCCAAGCCTGGCTGAAGGGACTAGTCTTGAAAACTAGCAGGGGCTTCACGGCCCGCGGGGGTTCGAATCCCTCATCACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 693922, 693924 ], "id": "EFPKPENHBP_26", "locus": "GCA000186005_01263" }, { "type": "tRNA", "sequence": "contig_1", "start": 693929, "stop": 694003, "strand": "-", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 82.6, "nt": "GGACCTATAGCTCAACGGCAGAGCAACCGGCTCATAACCGGTTGGTTACTGGTTCGAATCCAGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "EFPKPENHBP_25", "locus": "GCA000186005_01264" }, { "type": "tRNA", "sequence": "contig_1", "start": 694031, "stop": 694107, "strand": "-", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 78.8, "nt": "GGCGTTGTAGCTCAGTTGGTTAGAGCATCCGGTTCATACCCGGAAGGTCATGAGTTCGACTCTCATCGACGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 694080, 694082 ], "id": "EFPKPENHBP_24", "locus": "GCA000186005_01265" }, { "type": "tRNA", "sequence": "contig_1", "start": 694113, "stop": 694189, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 83.0, "nt": "GGGGGATTAGCTCATTTGGCTAGAGCACTTGCCTTGCACGCAAGGGGTGGTGGGTTCGAATCCCATATCCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 694158, 694160 ], "id": "EFPKPENHBP_23", "locus": "GCA000186005_01266" }, { "type": "tRNA", "sequence": "contig_1", "start": 694193, "stop": 694269, "strand": "-", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 81.9, "nt": "GGAGAAGTAGCTCAGCTTGGCAGAGCGCTACGTTTGGGACGTAGTGGTCGCAGGTTCAAATCCTGTCTTCTTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 694250, 694252 ], "id": "EFPKPENHBP_22", "locus": "GCA000186005_01267" }, { "type": "cds", "sequence": "contig_1", "start": 694575, "stop": 694811, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTTGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGA", "aa": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDH", "aa_hexdigest": "d5e72f9b9cdf92903d1042cffd5882ee", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9370.73569999999, "isoelectric_point": 8.831411170959473 }, "id": "EFPKPENHBP_1283", "locus": "GCA000186005_01268" }, { "type": "cds", "sequence": "contig_1", "start": 694851, "stop": 695615, "strand": "+", "frame": 3, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0", "UniRef:UniRef90_C4XDW6" ], "nt": "ATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGATGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATCAAGTGGCTTGA", "aa": "MVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHQVA", "aa_hexdigest": "951c9adc58108e834de067f91d4e433b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 694575, "stop": 695648, "observations": { "mutation": [ 694809, 694836, 695613 ], "stop": [ 694809, 694836, 695613 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDW6", "query_cov": 1.0, "subject_cov": 0.711484593837535, "identity": 1.0, "score": 508.0, "evalue": 2.75e-180, "gene_start": 1, "gene_end": 254, "reference_start": 93, "reference_end": 346, "reference_sequence": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDHWYKLRIKKDWFDLSMVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHQVAWLKEFVEQFKD" }, "paralog": false, "identity": 0.9915966386554622, "score": 704.0, "evalue": 5.22e-260, "description": "Internal stop codon at 694809, 694836, 695613. Nonsense mutation around 694809, 694836, 695613.", "psc": { "uniref90_id": "UniRef90_C4XDW6", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDW6", "UniRef:UniRef50_A0A3P8L7N0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0" ], "product": "Haloacid dehalogenase-like hydrolase" } }, "id": "EFPKPENHBP_1284", "locus": "GCA000186005_01269" }, { "type": "rRNA", "sequence": "contig_1", "start": 695808, "stop": 698691, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9859829059829059, "score": 2773.7, "evalue": 2.1e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TTTTAAATAAGGAAGAGTTTGTGGTGGATGCCTTGGGTCTGAAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGTGGAGCCGGAAATATGCTATGAAACGGGGATTTCCGAATGGGGAAACCTAACTAGGGTAATGCCTAGTTGCCTTGTTCTGAATTCATAGGAACTTGAGTGAGACACCTTGTGAACTGAAACATCTTAGTAGCAAGAGGAAGAGAAAATAAACAATGATTTCTTTAGTAGCGGCGAGCGAACGAGAAAGAGCCCAAACCCAGCAATGGGGGTTGTAGGACGATCTACATGAAGTTACAAAACTTAATGATAGCAGAAGCTTTTGGGAAGAAGCGGCATAGAGGGTGATACCCCCGTATGTGAAATTGTTAAGTCTTCTGATCGTATCCTGAGTAGGGCGGGGCACGTGGAACCCTGTCTGAATCTGCCGGGACCACCCGGTAAGGCTAAATACTAATCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGAGAGGGGAGTGAAATAGATTCTGAAACCACTTACTTACAATTAGTCAGAGCCCGTTTATGGGTGATGGCGTACATCTTGCAGTATGGACCGGCGAGTTATGACAGTATGCGAGGTTAAGCAGAAAAAAGCGGAGCCGTAGAGAAATCGAGTCTTAATAGGGCGTCTTAGTATGTTGTTATATACCCGAAACCATGTGATCTATTCATGAGCAGGCTGAAACTTGGCTAACCCCAAGCGGAGGGCCGAACCGTAGTACGCTGAAAAGTGCCCGGATGACTTGTGAATAGTGGAGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCCTCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAGTAGTGGTGGTAGAGCACTGAATATGGAATGGCCTCGCCTAGGGGTACTGACTATAATCAAACTCCGAATACCGCTATGTATTGCTATGCAGTCGGAACCGGGGTGCTAACGTCCCGGCTCGCGAGGGCAACAACCCAGACCGCCGGCTAAGGTCCCAAAATCGTGTTAAGTGAGAAAGGTTGTGAGGTTTCATAAACAACTAGGAAGTTGGCTCAGAAGCAGCCACCTTTTAAAGAGTGCGTAATAGCTCACTAGTCAAGAGACCTTGCGCCAACAATTTAACGGGACTAAAACACGATACCGAAGCCGCGGGTAATTATATTTACGTTAGAGGAGCGTTCCTAGGGCAATGAAGTCAGACCGTGAGGACTGGTGGAGCGCTAGGAAGTGAGAATGCCGGTATGAGTAACGATTCGTAGTGAGAATCTACGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGACCTAAGACGAGGCTGAAAAGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTATAATAGTGATGGAGTGACGGAGGAGGATAATCTTACCACTTACTGGATTGTGGGGTAAGCGTTAACTGGTGACTGTAGGCAAATCCGCAGTCTATAACCGGGAGGCGTGATGCATAGGGTCAAACCGAATTAGATAATTCCACACTTCCAAGAAAAGCTTCTAAACTTAATATTATGGAAACCTGTACCGAGAACGGACACACGTCCCCGAGATGAGTATTCTAAGGCGAGCGAGAAAACTAGTGTTAAGGAACTCTGCAAAATGACCCCGTAAGTTCGCAAGAAGGGGCGCCTAAGGTAACACTTAGGCCACAGTAAATTATGAGGGGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAACCGCAAGGTGATGTATAGGGGGTGAAGCCTGCCCAGTGCCCGAAGGTTAAGTGGATGCGTTAGCATTTGCGAAGCGTTGAAATGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGCGCAATGATCTCTCAACTGTCTCAACACTAGACTCGGTGAAATTATGGTCCCAGTGAAAACGCTGGGTACCCGCATCAAGACGAAAAGACCCCATGGAGCTTTACTACAGTTTCGTATTGGAACTTGGTCTAACATGTGTAGGATAGGTGGGAGACTTTGAAGCGAGGACGCTAGTTCTCGTGGAGTCATCCTTGAAATACCACCCTTGGTATATTGAGTTTCTAACATGCCATCATCATCAGGTGGGTGGACAGTGCGTGACGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGAGTAACGGAGGCGTTCAAAGGTACACTCAGTACGGTCAGAAACCGTATGTAGAGCGCAAAGGTAGAAGTGTGCTTGACTGTGAGACTTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGCTGACCAATACTAATAAATCGATCGGTTTAAAAGT", "id": "EFPKPENHBP_42", "locus": "GCA000186005_01270" }, { "type": "rRNA", "sequence": "contig_1", "start": 699005, "stop": 700517, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9869536855838226, "score": 1507.4, "evalue": 1.6e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGGCGTTAAATTTTGGGGCTCAACCCCAAAACGCGTTGGATACTGGCAGGCTAGAGTTGTGTAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAAGATGGCGAAGGCAGCTAACTGGACATATACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATCATTAGCTGATGGGGAACTCATCGGCGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTACGTTCGCAAGAATAAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATGGAGACATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCTGCAACGAGCGCAACCCTTATCCTTAGTTACTACCATTTAGTTGAGGACTCTAAGGAGACTGCCCGAGTAATCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCCGGTACAAAGAGAAGCGAAGTGGTGACATGGAGCAAACCTCAAAAAACCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTATAAACAAACTGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "EFPKPENHBP_38", "locus": "GCA000186005_01271" }, { "type": "cds", "sequence": "contig_1", "start": 700841, "stop": 700963, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00828.25" ], "nt": "ATGCCTGTTAAATTATTGGGTAATGGTCAAGTAACTAAAAAATTCAATGTTAATCTTCATGCAGCTTCAAAAAGTGCTATTGATGCAATCGAAGCTAAAGGTGGAAAAGTTACTTTATTATAG", "aa": "MPVKLLGNGQVTKKFNVNLHAASKSAIDAIEAKGGKVTLL", "aa_hexdigest": "c78cf49d5fc03939e8b08fbbd696d387", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00828.25", "name": "Ribosomal_L27A", "length": 37, "aa_cov": 0.925, "hmm_cov": 1.0, "evalue": 1.0458236070034506e-14, "score": 51.03211212158203, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4162.896099999999, "isoelectric_point": 10.000287437438963 }, "id": "EFPKPENHBP_1285", "locus": "GCA000186005_01272" }, { "type": "cds", "sequence": "contig_1", "start": 701111, "stop": 701281, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCAACATTACAATCATTAAGACCTACCGCTGGCTCACGTGTTGAAAAACACCGTGTTGGTCGCGGACATGCTGCTGGAAAAGGTAAACAAGCTGGTAAAGGTCAAAGTGGACAAAACAAACGTAAGGGTCACAGACTAGGATTTGAAGGTGGTCAAACACCTTGA", "aa": "MNSTLQSLRPTAGSRVEKHRVGRGHAAGKGKQAGKGQSGQNKRKGHRLGFEGGQTP", "aa_hexdigest": "dfca84a96eb9aa8097633e2899427aab", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5893.543500000002, "isoelectric_point": 11.999967765808105 }, "id": "EFPKPENHBP_1286", "locus": "GCA000186005_01273" }, { "type": "cds", "sequence": "contig_1", "start": 701336, "stop": 701554, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03719.21" ], "nt": "ATGCTTAAACCTGCTACAAAAGGTAAAGGTATTGTTGCTAGTGGTACAGTTAGAGCAGTTGTTGAACTTGCAGGTTACACCGATATTTATACCAAAACTTATGGTTCACGTTCAAAAGCAAACATTGTTAGAGCAACTTTAAAAGCTCTTCAAGCTTTAAGAACACCAGAACAAATTGCTGACATTCGTGGTAAAGATGTTAAAGATCTTTTAGCCTAA", "aa": "MLKPATKGKGIVASGTVRAVVELAGYTDIYTKTYGSRSKANIVRATLKALQALRTPEQIADIRGKDVKDLLA", "aa_hexdigest": "28fc6c4456239668fbf572f76a0c66b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03719.21", "name": "Ribosomal_S5_C", "length": 63, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 1.870331035803906e-26, "score": 87.71133422851562, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 7702.929999999999, "isoelectric_point": 10.070106697082519 }, "id": "EFPKPENHBP_1287", "locus": "GCA000186005_01274" }, { "type": "cds", "sequence": "contig_1", "start": 701921, "stop": 702046, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAGAAGTAAAGAAAGTTGTTAATAAAGAACAACCTAAAGAAGCCCAAGCTCAAGGAACTAAAGAAGTTGTAGCTCCTAAAGCTAAAGCTGAAAACAAAGATAAAAAACAAATTTGA", "aa": "MAEAEVKKVVNKEQPKEAQAQGTKEVVAPKAKAENKDKKQI", "aa_hexdigest": "4287dce5a67b631dc5ca2eaa847fb5dc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4520.171200000001, "isoelectric_point": 9.399055290222169 }, "id": "EFPKPENHBP_1288", "locus": "GCA000186005_01275" }, { "type": "cds", "sequence": "contig_1", "start": 702049, "stop": 702414, "strand": "-", "frame": 1, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354765.1", "SO:0001217", "UniParc:UPI0001E32EBC", "UniRef:UniRef100_C4XFZ9", "UniRef:UniRef50_Q4A5D7", "UniRef:UniRef90_A0A3P8LIJ1" ], "nt": "ATGGCTACATTATCTCGTAATCAAGCACGTCAAGTTAAACACTTACGTGAACGTCAAAGTATTTCAGGTACTGCTACTAAACCACGTCTTTGCGTATTCAAATCTCATACAAACTTTTATGCACAACTAATTGACGATACAACAGGACACACTTTAGCAAGTGTTTCAACACTTGATCCTAAAGCTAAAGTTAAATACCACGGAAACATTGCTTCAGCAACTAAAGCTGGTGAAGCAATGGGTGCTAAAATTGCAGCCCTTAAATTAAAAGAAATAGTATTCGATCGTTCAGGTTACATTTATCATGGACGTGTTAAAGCCTTCGCAGAAGGTGTTAGAAGTATAGCTAAAGGAGTTAAATTCTAA", "aa": "MATLSRNQARQVKHLRERQSISGTATKPRLCVFKSHTNFYAQLIDDTTGHTLASVSTLDPKAKVKYHGNIASATKAGEAMGAKIAALKLKEIVFDRSGYIYHGRVKAFAEGVRSIAKGVKF", "aa_hexdigest": "58b59e311212f3d90497aedf3886588a", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EBC", "ncbi_nrp_id": "WP_013354765.1", "uniref100_id": "UniRef100_C4XFZ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EBC", "RefSeq:WP_013354765.1", "UniRef:UniRef100_C4XFZ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ9", "uniref90_id": "UniRef90_A0A3P8LIJ1" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LIJ1", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q4A5D7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D7" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "EFPKPENHBP_1289", "locus": "GCA000186005_01276" }, { "type": "cds", "sequence": "contig_1", "start": 702441, "stop": 702980, "strand": "-", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_013526982.1", "SO:0001217", "UniParc:UPI0001F59BEB", "UniRef:UniRef100_A0AB32XC65", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_C4XG00" ], "nt": "ATGTCTCGTGTTGGAAATAGAGTATTAAATATCCCTGCAGGTGTTGAAGTTAATTTAAATGGTACAACCTTAAATGTTAAAGGTTCATTAGGCCAATTAGAAAAAACATTCAGCCCACTTATTGCAATTAAAATTGAAAATAATCAAATTACAACCCTCCGTGCTAATGAAGAAAAACACACAAAACAATTACACGGAACAACAAATGCTTTAATTCAAGGCATGCTTGAAGGTGTTTCAAAAGGTTTCAAAAAAGAATTAGCAATCAAAGGTGTTGGTTACAAAGCTGCACTTAATGGTCAAACTTTAGAATTAACTGTTGGTTGCAGCCACATAGTTAGTCTAAGTGTACCAGAAGGTGTTAAAGTTGAAGTTCCAAAACCTCTTGAAATCATTATTAGTGGTATTGACAAAGAAAAAGTTGGTCAATTTGCTGCTGTTGTGCATGATGTTCGTCGTCCTAACTCATACTCAGGTAAAGGGATTTCATACAAAGGCGAATACATTCGTATTAAAGAAGGAAAAACTGCTTCTAAATAG", "aa": "MSRVGNRVLNIPAGVEVNLNGTTLNVKGSLGQLEKTFSPLIAIKIENNQITTLRANEEKHTKQLHGTTNALIQGMLEGVSKGFKKELAIKGVGYKAALNGQTLELTVGCSHIVSLSVPEGVKVEVPKPLEIIISGIDKEKVGQFAAVVHDVRRPNSYSGKGISYKGEYIRIKEGKTASK", "aa_hexdigest": "f50c4f85b770dd8d5f69be8bd533be29", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001F59BEB", "ncbi_nrp_id": "WP_013526982.1", "uniref100_id": "UniRef100_A0AB32XC65", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BEB", "RefSeq:WP_013526982.1", "UniRef:UniRef100_A0AB32XC65" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC65", "uniref90_id": "UniRef90_C4XG00" }, "psc": { "uniref90_id": "UniRef90_C4XG00", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "EFPKPENHBP_1290", "locus": "GCA000186005_01277" }, { "type": "cds", "sequence": "contig_1", "start": 702995, "stop": 703387, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_C4XG01" ], "nt": "ATGTTTATCACAGATCCAATTTCAGATATGATTGTTAGAATCAAAAACGCTAATCAACGTAAATTCAAAACTGTAGCTATTCCTTTTTCAAATAAAAAAGCTAAAATTCTAGAAATTCTTCAAAATGAAGGTTACATTACAAACTTTGTTACAAAAGGCCAAGGTAAAGACAAAGTACTTGAAGTTTCATTAAAATACAAAAACAACCAAAGTGCAATTGTTGACTTTAAACGTGTTTCAAAACCAGGTCTTAAAGTTTATACTTCAGCTGACAAATTACCTACCATTTTATCAGGTTATGGTACAGCAATCATTTCAACTTCAAAAGGAATGATGACTGAAAAACAAGCTCGTAAGGAAAATGTGGGTGGTGAAATCATTGCCTACATTTGA", "aa": "MFITDPISDMIVRIKNANQRKFKTVAIPFSNKKAKILEILQNEGYITNFVTKGQGKDKVLEVSLKYKNNQSAIVDFKRVSKPGLKVYTSADKLPTILSGYGTAIISTSKGMMTEKQARKENVGGEIIAYI", "aa_hexdigest": "a5856a56c4197a0d5098d6f8d5af4378", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XG01", "query_cov": 1.0, "subject_cov": 0.9701492537313433, "identity": 1.0, "score": 250.0, "evalue": 4.25e-84, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "EFPKPENHBP_1291", "locus": "GCA000186005_01278" }, { "type": "cds", "sequence": "contig_1", "start": 703393, "stop": 703575, "strand": "-", "frame": 1, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_A5IYX3", "UniRef:UniRef90_A5IYX3" ], "nt": "ATGGCTAAAGTTTCATTAAAAGCAAAACAAAAGAAACATCCTAAATTCTCAACACGTGCTTACACACGTTGTGAATTATGTGGACGTCCACACGCAGTTTTAAGAAAATACAAGATTTGCCGTATTTGTTTCCGTAATTTAGCTCATGAAGGAAAAATTCCTGGCATGAAGAAAGCGAGTTGA", "aa": "MAKVSLKAKQKKHPKFSTRAYTRCELCGRPHAVLRKYKICRICFRNLAHEGKIPGMKKAS", "aa_hexdigest": "d8ef3eb6eb98730ca40562b821f7b0c7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A5IYX3", "query_cov": 1.0, "subject_cov": 0.8823529411764706, "identity": 1.0, "score": 127.0, "evalue": 2.79e-37, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_A5IYX3", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX3" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [ "rpsZ" ], "id": "EFPKPENHBP_1292", "locus": "GCA000186005_01279" }, { "type": "cds", "sequence": "contig_1", "start": 703577, "stop": 703804, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "nt": "ATGCCACGTATTCGTGATTTCCGTGGTGCCAACCCTAAAGCATTCGATGGTAGAGGAAACTACTCATTAGGTGTTAAAGAAGAAATTATTTTCCCAGAAATCGAATTTGACAAAATTCGTCACATCAAGGGACTTGATGTTCAATTAATCACAAGCACAAATAAAGATGAAGAAGCTAGAAGTTTATTAGAATTAATCGGCATTCCATTTATTAAAGGAGATAGATAA", "aa": "MPRIRDFRGANPKAFDGRGNYSLGVKEEIIFPEIEFDKIRHIKGLDVQLITSTNKDEEARSLLELIGIPFIKGDR", "aa_hexdigest": "e529e4da82ed9014b0ac75de865e36ee", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NY94", "query_cov": 0.9866666666666667, "subject_cov": 0.9866666666666667, "identity": 0.595, "score": 104.0, "evalue": 6.4e-28, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A3B0NY94", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NY94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "EFPKPENHBP_1293", "locus": "GCA000186005_01280" }, { "type": "cds", "sequence": "contig_1", "start": 703895, "stop": 704122, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGAATTTAAAAGCTTATTACACAAAAACAGTTGTACCTGCATTAATGAAAAAATATAACTATTCATCAGTTATGCAAGTACCACGTTTAGAAAAAATTGTTTTGAATATGACAGCAGGTAAAGAAGTAACAAACTCTAAAGCTATCGAAGAAGTTCTTAATGAACTTACTCAAATTTCAGGTCAAAAACCATTCCAAACCAGAGCTAAAAAATCAAATGCTTCATGA", "aa": "MNLKAYYTKTVVPALMKKYNYSSVMQVPRLEKIVLNMTAGKEVTNSKAIEEVLNELTQISGQKPFQTRAKKSNAS", "aa_hexdigest": "fa8b97cf1baa8f1c4b7da190658b4a16", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.9333333333333333, "subject_cov": 0.8333333333333334, "identity": 0.586, "score": 83.6, "evalue": 1.39e-19, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "EFPKPENHBP_1294", "locus": "GCA000186005_01281" }, { "type": "cds", "sequence": "contig_1", "start": 704125, "stop": 704451, "strand": "-", "frame": 1, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354770.1", "SO:0001217", "UniParc:UPI0001E32EC1", "UniRef:UniRef100_C4XG04", "UniRef:UniRef50_Q98PZ3", "UniRef:UniRef90_C4XG04" ], "nt": "ATGAAATTAAAACTTAAGAAGAACGACGAAGTTATTGTTATTGCAGGAAGCAATAAAGGTAAAATTGGTCGTATTATTAGAACAAATTTTAAAACAAACCGTGCATATGTAAAAGATGTAAACGTAGTAACTAAACACGTAAAACCAAGTCAAAGTAATACAGAAGGTTCAATCAAGAACGTTGAAGCTCCTATTCACATTTCAAATCTTGCAATCGTTACTAAAAAAGCTACCAAAAGTGGTGCTGCTCAATATTCAAAAGTTGGTTTTAAAGTTAAAGGTAACACTAAAGTTAGAGTTGCTCGTAAAACTAAGAAGGAAATTTAA", "aa": "MKLKLKKNDEVIVIAGSNKGKIGRIIRTNFKTNRAYVKDVNVVTKHVKPSQSNTEGSIKNVEAPIHISNLAIVTKKATKSGAAQYSKVGFKVKGNTKVRVARKTKKEI", "aa_hexdigest": "3e73b247f84e4d4d23a189070ede1090", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001E32EC1", "ncbi_nrp_id": "WP_013354770.1", "uniref100_id": "UniRef100_C4XG04", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC1", "RefSeq:WP_013354770.1", "UniRef:UniRef100_C4XG04" ] }, "ips": { "uniref100_id": "UniRef100_C4XG04", "uniref90_id": "UniRef90_C4XG04" }, "psc": { "uniref90_id": "UniRef90_C4XG04", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_Q98PZ3", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ3" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "EFPKPENHBP_1295", "locus": "GCA000186005_01282" }, { "type": "cds", "sequence": "contig_1", "start": 704473, "stop": 704841, "strand": "-", "frame": 1, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_013354771.1", "SO:0001217", "UniParc:UPI0001E32EC2", "UniRef:UniRef100_A0AB32XC52", "UniRef:UniRef50_Q85CT4", "UniRef:UniRef90_C4XG05" ], "nt": "ATGGTTATAGCACTTTCTAAATTAAATGTTGCAGATAACTCTGGAGCAAAAGAAGTTGGTGTAATCAAAGTTTTAGGTGGAAGTAGAAAGAAAACAGCTAATATCGGTGACGTTATTATCTGTTCAGTTAAAAAAGCTTTACCTAATGGTTTAGTAAAAGAAGGACAAGTAGTTAAAGGTGTTATTGTTAGAAGTGTTTATGGAATTAGCCGTGAAAATGGTACACATATTCGTTTCGACGATAATGCAGTTGTGTTAATTAAAGATGATGGTACTTTAAGAGGTACTCGTGTGTTTGGACCTGTTGCTCGTGAATTACGTGACCGTGGTTATTTAAAAATCGTTTCATTAGCACCTGAAGTTCTTTAA", "aa": "MVIALSKLNVADNSGAKEVGVIKVLGGSRKKTANIGDVIICSVKKALPNGLVKEGQVVKGVIVRSVYGISRENGTHIRFDDNAVVLIKDDGTLRGTRVFGPVARELRDRGYLKIVSLAPEVL", "aa_hexdigest": "e3333d702fd7dd8deb44c64a446d5a98", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32EC2", "ncbi_nrp_id": "WP_013354771.1", "uniref100_id": "UniRef100_A0AB32XC52", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC2", "RefSeq:WP_013354771.1", "UniRef:UniRef100_A0AB32XC52" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC52", "uniref90_id": "UniRef90_C4XG05" }, "psc": { "uniref90_id": "UniRef90_C4XG05", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q85CT4", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "genes": [ "rplN" ], "id": "EFPKPENHBP_1296", "locus": "GCA000186005_01283" }, { "type": "cds", "sequence": "contig_1", "start": 704841, "stop": 705107, "strand": "-", "frame": 2, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354772.1", "SO:0001217", "UniParc:UPI0001E32EC3", "UniRef:UniRef100_C4XG06", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_C4XG06" ], "nt": "ATGGAAAGAAGTTCACGTAAGACCCTTCAAGGTCGTGTTGCTTCAGCACGTGGTGATAAAACTATCATCGTTGAAGTTGAAAGTTATAAAGCTCATAGTCTTTACTCAAAACGTTTTAAAGTTGTTAAACGTTTCGCAGTTCATGATGAAAAAAACTTAGCTAAAGTTAACGACATCGTTACAATTATGGAAACACGTCCTCTTTCAAAAACCAAACACTTCCGTCTTGTTGAAATTAAACAACACGCAGTTGAAGGAGAAAAATAA", "aa": "MERSSRKTLQGRVASARGDKTIIVEVESYKAHSLYSKRFKVVKRFAVHDEKNLAKVNDIVTIMETRPLSKTKHFRLVEIKQHAVEGEK", "aa_hexdigest": "9988ace3ccb6a0572c22a13f19cba37f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC3", "ncbi_nrp_id": "WP_013354772.1", "uniref100_id": "UniRef100_C4XG06", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC3", "RefSeq:WP_013354772.1", "UniRef:UniRef100_C4XG06" ] }, "ips": { "uniref100_id": "UniRef100_C4XG06", "uniref90_id": "UniRef90_C4XG06" }, "psc": { "uniref90_id": "UniRef90_C4XG06", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "EFPKPENHBP_1297", "locus": "GCA000186005_01284" }, { "type": "cds", "sequence": "contig_1", "start": 705107, "stop": 705310, "strand": "-", "frame": 3, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354773.1", "SO:0001217", "UniParc:UPI0001E32EC4", "UniRef:UniRef100_C4XG07", "UniRef:UniRef50_A5IYX8", "UniRef:UniRef90_C4XG07" ], "nt": "ATGCTTTATAAAGATATTAAAGTTAAAAATAATGCTGAACTCCGTAAATTACTTGACGATTTAAAAGCACAATTATTTATTTATCGTTTCCAAAACAAAACTGGGACATTAGATCACAGTCACAAAATTAGAGAAGTACGTCGTGACATTGCTAAAGTATTAACAGTTTTAAAAATTAACGAATCTAAAGGAGGAGCTAAATAA", "aa": "MLYKDIKVKNNAELRKLLDDLKAQLFIYRFQNKTGTLDHSHKIREVRRDIAKVLTVLKINESKGGAK", "aa_hexdigest": "37e9c9e6d745504fa848d9e6f84a8d70", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC4", "ncbi_nrp_id": "WP_013354773.1", "uniref100_id": "UniRef100_C4XG07", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC4", "RefSeq:WP_013354773.1", "UniRef:UniRef100_C4XG07" ] }, "ips": { "uniref100_id": "UniRef100_C4XG07", "uniref90_id": "UniRef90_C4XG07" }, "psc": { "uniref90_id": "UniRef90_C4XG07", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A5IYX8", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX8" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "EFPKPENHBP_1298", "locus": "GCA000186005_01285" }, { "type": "cds", "sequence": "contig_1", "start": 705454, "stop": 705561, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGTCGTGAAGGTCAAGTTTTCATTAGAATCTTCCCTCACTTCTCAAAAACATCAAAACCTATCGGTGTTCGTATGGGTTCTGGTAAAGGTAGTCCTGAAAAATGA", "aa": "MGREGQVFIRIFPHFSKTSKPIGVRMGSGKGSPEK", "aa_hexdigest": "697d90d1d37e401998077d88652ea801", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.1390569890759957e-10, "score": 36.4363899230957, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3847.4728000000005, "isoelectric_point": 11.068786430358884 }, "id": "EFPKPENHBP_1299", "locus": "GCA000186005_01286" }, { "type": "cds", "sequence": "contig_1", "start": 705610, "stop": 705735, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAATATAGAAAACCATTTTTAGTACGTCATGACAAACGTAAAGCCTTCAAAGGTAATACAGTTGCTTATGGTGACTTTGGTTTACAAGCTGTAAGCTCAGCTTGA", "aa": "MLQPKRTKYRKPFLVRHDKRKAFKGNTVAYGDFGLQAVSSA", "aa_hexdigest": "a28e4943ebc9e731e4abe2f6b2bfc039", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.454900000001, "isoelectric_point": 10.74824962615967 }, "id": "EFPKPENHBP_1300", "locus": "GCA000186005_01287" }, { "type": "cds", "sequence": "contig_1", "start": 705773, "stop": 705892, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "ATGGCTAGAACAGAAGGCTATTCACGTGGAGAAATGAGACTTCACACATTAAGACAAGATGTTGATTATGCACACGCAAATGCTCACACTACTTATGGTGTAATCGGCGTTAAAGTGTGA", "aa": "MARTEGYSRGEMRLHTLRQDVDYAHANAHTTYGVIGVKV", "aa_hexdigest": "6bec5ed16b8584a907b907b62f925a05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 39, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 5.114048353796125e-15, "score": 52.15079879760742, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4404.901, "isoelectric_point": 8.247198677062986 }, "id": "EFPKPENHBP_1301", "locus": "GCA000186005_01288" }, { "type": "cds", "sequence": "contig_1", "start": 706394, "stop": 706753, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02890", "RefSeq:WP_013354776.1", "SO:0001217", "UniParc:UPI0001E32EC7", "UniRef:UniRef100_C4XG10", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_C4XG10" ], "nt": "ATGGCACAAAATACACCAGTTGTTCAACAAGCTAAAGCTCATGTTGAAATTCAACGTGTTAGTGCACGTAAAGCACGTTTAGTTGCTGACTTAGTTAGATCTAAAGACGTGCGTGAAGCTCTTGGTATTTTAAGAAATACAAACAAGAAATCTTCAGAATTATTTATTAAACTTATTAACTCAGCAATTGCTAATGCTACAAACAACCACGGAATGGATGCATCTAAATTATTTGTTAAAGAAATCTTAGTTAATGAAGGACCAACACTTAAAAGATACCAACCTCACTCAAGAGGAACAGCTTATTCAATTTTTAAACGTACATCACATTTATCAATTACATTAGAAGAAAGAAAGTAG", "aa": "MAQNTPVVQQAKAHVEIQRVSARKARLVADLVRSKDVREALGILRNTNKKSSELFIKLINSAIANATNNHGMDASKLFVKEILVNEGPTLKRYQPHSRGTAYSIFKRTSHLSITLEERK", "aa_hexdigest": "eb44942af9594e8c854873f987f7fb42", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32EC7", "ncbi_nrp_id": "WP_013354776.1", "uniref100_id": "UniRef100_C4XG10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC7", "RefSeq:WP_013354776.1", "UniRef:UniRef100_C4XG10" ] }, "ips": { "uniref100_id": "UniRef100_C4XG10", "uniref90_id": "UniRef90_C4XG10" }, "psc": { "uniref90_id": "UniRef90_C4XG10", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "EFPKPENHBP_1302", "locus": "GCA000186005_01289" }, { "type": "cds", "sequence": "contig_1", "start": 706927, "stop": 707031, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAGTCTTAAAAAAGGACCTTTTGCAGACGAACATTTACTTAAAAAAGTAGATGCTATTGTTGAAGGCAAAGCGCCAAAAAAACCAATTAAAACTTGA", "aa": "MARSLKKGPFADEHLLKKVDAIVEGKAPKKPIKT", "aa_hexdigest": "159a49d0b0c41ac497ccc03212c29f2d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.4823000000015, "isoelectric_point": 9.998095512390137 }, "id": "EFPKPENHBP_1303", "locus": "GCA000186005_01290" }, { "type": "cds", "sequence": "contig_1", "start": 707118, "stop": 707876, "strand": "-", "frame": 2, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_D5E4V9" ], "nt": "ATGGCAATTAAACATTACAAGCCAACTACGAATGGACGTCGTAATATGTCATCTCTACATTACACAGAAAACTTAAGTGGACATGCTCCTGAAAAATCTTTACTTACACTATTAAAATATAGTGCTGGTCGCAATAACCAAGGTAAGATTACAGTACGTCACAAGGGTGGTCGTGTTAAGAGATTTTACAGATTAATTGACTTCAAACGTAATAAGGACAATATTCCAGCAGTAGTTAAGACCATTGAATATGATCCTAACCGTTCAGCTAATATTTGTTTATTAGCATATGCTGATGGAGAAAAAAGGTATATTTTAGCGCCTGAAGGCATTAAAGTAGGCCAAAGCGTTATTTCAGGTGAACACGCTGACATTTTAGTAGGTAATGCATTACCACTTTCAAGCATTCCAGAAGGTACAATTGTACACAACATTGAAATGCAACCAGGTGGTGGAGGTATTATTGCTAGATCAGCAGGTACATCTGCACAAATCTTAGGTAAAGATGACGACGGTAAATATGTTGTTTTACGTCTAAAAAGCGGTGAAATGCGTCGTATCTTAGCTCGTTGCCGTGCAACTATCGGTTCAGTTGGTAACGAAGAACATTTATTGGTTGATTTAGGAAAAGCTGGTAGAAACCGTTATAAAGGTGTTCGTCCAACAGTTCGTGGTTCAGTTATGAACCCTGTGGATCACCCACATGGTGGTGGTGAAGGTAAACAACCTGTTGGTCGTAAAGCTCCTCTTACTCCTTGA", "aa": "MAIKHYKPTTNGRRNMSSLHYTENLSGHAPEKSLLTLLKYSAGRNNQGKITVRHKGGRVKRFYRLIDFKRNKDNIPAVVKTIEYDPNRSANICLLAYADGEKRYILAPEGIKVGQSVISGEHADILVGNALPLSSIPEGTIVHNIEMQPGGGGIIARSAGTSAQILGKDDDGKYVVLRLKSGEMRRILARCRATIGSVGNEEHLLVDLGKAGRNRYKGVRPTVRGSVMNPVDHPHGGGEGKQPVGRKAPLTP", "aa_hexdigest": "054fb9374afee5cadbbdf737004e8920", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D5E4V9", "query_cov": 1.0, "subject_cov": 0.8904593639575972, "identity": 1.0, "score": 503.0, "evalue": 1.17e-179, "valid": true, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "EFPKPENHBP_1304", "locus": "GCA000186005_01291" }, { "type": "cds", "sequence": "contig_1", "start": 707948, "stop": 708226, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTTTAGCTGAAGGAGATAAAATCGTTTATTATCCAAATGAAGCTGAAGCTCAAGATGCTAAAAAAGAAGCTAAAGTTAAAGCTAAAAAAGCTGAAGATAAAAAACAAGCAGCAGCTAAAGAAGCAGAAATTGCTAAAAAACTTGCTGCTAAAAAAGCTAAAGCAGCTGAAAGCAAAAAAGCAGACAGCTCAAAAGCTGAAAAAACTACAAAATCAACTACTTCAAAACCTGCTGCTAAAAAAGTTGCAGCTGCTAAGAAGAGTTCAAAATAA", "aa": "MITLAEGDKIVYYPNEAEAQDAKKEAKVKAKKAEDKKQAAAKEAEIAKKLAAKKAKAAESKKADSSKAEKTTKSTTSKPAAKKVAAAKKSSK", "aa_hexdigest": "792aa382020172fe834ae68c3dcba999", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9736.16699999999, "isoelectric_point": 9.875154304504395 }, "id": "EFPKPENHBP_1305", "locus": "GCA000186005_01292" }, { "type": "cds", "sequence": "contig_1", "start": 708520, "stop": 708693, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAATCTCAACTAAAGATGCTGTTAACAAACTTAAATCATTAAAAATTGAAAACTTAAAACACATTTTAGTGGTAACAACAGATGAAACTGTTTACAAATCAACAGCTAACTTACAAAATGTTTTATGTGTTAAACCAAACTCAGTAAGTGTTGAAAACCTTGTTTGA", "aa": "MSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLV", "aa_hexdigest": "f17e344e8b3a504831f257ea37f66ff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6299.3160000000025, "isoelectric_point": 9.25226078033447 }, "id": "EFPKPENHBP_1306", "locus": "GCA000186005_01293" }, { "type": "cds", "sequence": "contig_1", "start": 708889, "stop": 709593, "strand": "-", "frame": 1, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_C4XG14", "UniRef:UniRef90_C4XG14" ], "nt": "ATGGCTGAAACAAAGAAAAAAGCTACTACTAAAGCTAGTGAAGAAAAGAAAACTATAACTAAAAAAGTAGCAACTGCTAAGAAAACTACTACTGCTAAATCAACTTCTACAGCTAAAAAATCAACAACTACTGCTAAAAAAGCTACTACAACAACTAAAAAATCCGCTGCTCCTAAAGCAACAGCTGAAAAGAAAACAACTTCAAGAAAAAAAGTTACAGTTGAAATTGATTTAACTAAAAACTTAAAACCTAAAAAGCCTACTAAAGCTGAATTAAAAGCTCAAGAAGCTAAAGAAGAAAGTGCTAAAAAGAAAACCGCTGCTAAAACAGCTGCTCAAAAAGCTAAATTAGCTAAAGAAGAAGCTGCTGCAAAAGCAAAAGCAGAAAAAGAAGCACAAGCAGCTTTAGATGAATTAAAAGCTGCAGCAGAAGCTGCTAAAAGAAAAGCTAAAACTTATGCATCTTCAAGAGCAAAAGTTGCTTCACTTAAATTAAATGATGCTAAACTTGAACAAGAAAAATTTACAAAAGTTATCTTGCTTCACTTTGACAATAAAGCCTTACCAAAAGAATTATTTGCATCACCAAAAATTTATGAACAAGCAATTTTTGACACCATTATGGCTGATAGAGCATCAAGACGTCAAGGTACTCATGATGTTAAAAATCGTTCAGAAGTAAGCGGTGGTGGTAAAAAACCTTGA", "aa": "MAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKP", "aa_hexdigest": "9ae2affe01022b21dd6220ad6a3783ba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 708586, "stop": 709596, "observations": { "mutation": [ 708837, 708891 ], "stop": [ 708837, 708891 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG14", "query_cov": 1.0, "subject_cov": 0.6190476190476191, "identity": 1.0, "score": 215.0, "evalue": 4.45e-65, "gene_start": 1, "gene_end": 234, "reference_start": 2, "reference_end": 235, "reference_sequence": "MMAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKPWRQKGTGRARAGSTRSPIWVGGGRAFGPTPERNYSLKVNKKVRRAAFLSALTLLSNKKSVVIDDFKMSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLVWADVLVLSNEGLKNFEGRIK" }, "paralog": false, "identity": 0.9880952380952381, "score": 410.0, "evalue": 3.58e-144, "description": "Internal stop codon at 708837, 708891. Nonsense mutation around 708837, 708891.", "psc": { "uniref90_id": "UniRef90_C4XG14", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_C4XG14", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG14", "UniRef:UniRef50_C4XG14", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG14" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "EFPKPENHBP_1307", "locus": "GCA000186005_01294" }, { "type": "cds", "sequence": "contig_1", "start": 709593, "stop": 709940, "strand": "-", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGCCAGGTCACTTAGGAGCTGTTAGAACAACAGTTCAAAATCTTGAAATTGTTAAAGTTGATGTAGCAAACAACATTATGTTAGTTAAAGGATCAATTCCTGGGGCTAAAAATTCATATGTTATGATTCGTGAAGCTGTTAAAGGCTTACCAAATCATGATGCAATTAAACTTGTTGATATTAAAGAAGTTCTTAAAATGAATGAATTGCTTGAAAGAGCTAAAAAATACAACATTGAAGTTACAGTTGGTATGCATTCAAGTGAATTAGAACCTCTTATCCAAAAAGCTGAAGCTGATGCAGCTGCAGCAGCTGAAGAAAGTAAAAAAGCAGAAGGAGATAAATAA", "aa": "MPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK", "aa_hexdigest": "acab8c86c449961495c19a7a3340cc7a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 709593, "stop": 710240, "observations": { "mutation": [ 710054 ], "stop": [ 710054 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4166666666666667, "identity": 1.0, "score": 212.0, "evalue": 2.72e-67, "gene_start": 1, "gene_end": 115, "reference_start": 162, "reference_end": 276, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9953488372093023, "score": 420.0, "evalue": 1.94e-151, "description": "Internal stop codon at 710054. Nonsense mutation around 710054.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "EFPKPENHBP_1308", "locus": "GCA000186005_01295" }, { "type": "cds", "sequence": "contig_1", "start": 710052, "stop": 710420, "strand": "-", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGAAAGGAATCTTAGGACGGAAGATTGGTATGACACAAATCTTTACTGAACTTGGAGCTAGTGTTCCAGTTACAGTTATAGAAGTGCAACCAAATGTTGTTACTAAAGTTCTTTCAGCTGACAAAAATGGGTATGTTGCAACCCAAATTGCTGTTGGTGAAAAGAAAGAACACAGAAGCAATAAACCTCTTAAAGGTCAATTTGCTCAAGCTAAAACAACACCTAAGCGCTACGTTAAAGAAATTCGTGGCATGCAAGGCTATGAATTAGGTCAAACTATTAAGGCCAACATTTTTAAAGCTGGTGAATTAGTAGATATCACTGGTACATCAAAAGGTAAAGGTTTTGCCGGTACAATCAAAAGATGA", "aa": "MKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKR", "aa_hexdigest": "5cd896c66477f169406bf9b1ac3518b2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 709749, "stop": 710423, "observations": { "mutation": [ 710054 ], "stop": [ 710054 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4420289855072464, "identity": 1.0, "score": 233.0, "evalue": 2.33e-75, "gene_start": 1, "gene_end": 122, "reference_start": 2, "reference_end": 123, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9910714285714286, "score": 437.0, "evalue": 3.61e-158, "description": "Internal stop codon at 710054. Nonsense mutation around 710054.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "EFPKPENHBP_1309", "locus": "GCA000186005_01296" }, { "type": "cds", "sequence": "contig_1", "start": 710492, "stop": 710797, "strand": "-", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354782.1", "SO:0001217", "UniParc:UPI0001A8C186", "UniRef:UniRef100_C4XG16", "UniRef:UniRef50_Q98PX9", "UniRef:UniRef90_C4XG16" ], "nt": "ATGAAAAAAATCAATATCAAGGTTAAAAGTTTCGATCATGCTTTAGTTGATTTTGGAGCAAAGAAAATTGTTGAAGTTGCTACCAAAAACTCAGTTAAAGTAAGTGGTCCAGTTCCTCTTCCAACAAAAAGAGAAGTTGTGACTATCTTAAGATCAGTTCACGTTAACAAAAAATCTCGTGAACAATTTGAATCAAGAACTTTTCAACGTTTGATCGTTTTATTAAACCCAAACGATAAAGCTCTTGACCAAATTAAGAGATTAGAACTTCCAGCAGGTGTTGAAGTTCAAATCTCAACAAAATAA", "aa": "MKKINIKVKSFDHALVDFGAKKIVEVATKNSVKVSGPVPLPTKREVVTILRSVHVNKKSREQFESRTFQRLIVLLNPNDKALDQIKRLELPAGVEVQISTK", "aa_hexdigest": "15182ac8da1878398a66916b08ae983e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C186", "ncbi_nrp_id": "WP_013354782.1", "uniref100_id": "UniRef100_C4XG16", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C186", "RefSeq:WP_013354782.1", "UniRef:UniRef100_C4XG16" ] }, "ips": { "uniref100_id": "UniRef100_C4XG16", "uniref90_id": "UniRef90_C4XG16" }, "psc": { "uniref90_id": "UniRef90_C4XG16", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_Q98PX9", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PX9" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "EFPKPENHBP_1310", "locus": "GCA000186005_01297" }, { "type": "cds", "sequence": "contig_1", "start": 711427, "stop": 711879, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTCTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACTCAGATCAAAATATACCTAACATTACAGTTAGCGCTAGTGAAAAAGGTTTAGGCTACTATCCAAAAACAATACAAAGATTAATTAAAGGCAAAAAAAATAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAATGTTTATCATGATGATATACACAAATTTGATAGACTTGAGAAAGTTTACTTTTGA", "aa": "MKKKLFFNFSLLSSVALPLTTIAASCNQNSDQNIPNITVSASEKGLGYYPKTIQRLIKGKKNKELIDTINQFINNKEQIKYENNKEYSLNENESTFGYNELKKEYKCIIALNIKDNDKTKVKKFSINKIGHNNVYHDDIHKFDRLEKVYF", "aa_hexdigest": "64dcfc6ace6f2d782e9f968980b0b097", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17476.774599999997, "isoelectric_point": 9.345546531677247 }, "id": "EFPKPENHBP_1311", "locus": "GCA000186005_01298" }, { "type": "cds", "sequence": "contig_1", "start": 712345, "stop": 712572, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGA", "aa": "MTSKDFLDLLDKVDKEHNHEPKDIINFLKQYVNIDGTYDANDKYEYAFNVEKTHTHGSGQLHLYTYYREKGKNQK", "aa_hexdigest": "137ca94b2b95e6cad4d06177a5b7958a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.7844, "isoelectric_point": 6.165973472595215 }, "id": "EFPKPENHBP_1312", "locus": "GCA000186005_01299" }, { "type": "cds", "sequence": "contig_1", "start": 712618, "stop": 712833, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGA", "aa": "MGGLKFNNAGIKDAKQANEDPGFYTSPHQILREMETKSTFDEQLAVLKKYCEVKDESDKNKYDYKFNFKES", "aa_hexdigest": "49406b5a7f89a963e2a6e0704ca5ebec", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8260.130999999998, "isoelectric_point": 5.769635200500488 }, "id": "EFPKPENHBP_1313", "locus": "GCA000186005_01300" }, { "type": "cds", "sequence": "contig_1", "start": 713656, "stop": 713787, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGA", "aa": "MRLDLKNFGAFSKVGDFLIKNTPSETDIVNVRAQDIINKAKSI", "aa_hexdigest": "4722c44c811fc246110dd46ef1c40979", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4808.513800000001, "isoelectric_point": 9.397443580627442 }, "id": "EFPKPENHBP_1314", "locus": "GCA000186005_01301" }, { "type": "cds", "sequence": "contig_1", "start": 713863, "stop": 714036, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTAAAGAACACTAAAACAAATGAAGAAGAAAATGTTTATATTCTATTTTGCAAATTAGCCTAA", "aa": "MGFPGYDNTVINEDLEYTIDFNKAVVYKSGKLELNIKVKNTKTNEEENVYILFCKLA", "aa_hexdigest": "51ce64c015467534b7f401ad78473eaf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6578.4301, "isoelectric_point": 4.893632698059082 }, "id": "EFPKPENHBP_1315", "locus": "GCA000186005_01302" }, { "type": "cds", "sequence": "contig_1", "start": 714778, "stop": 715665, "strand": "+", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_Q07809", "UniRef:UniRef90_C4XG20" ], "nt": "ATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCAATTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGA", "aa": "MKSKNIEIKNKDKELKEALEEITKKFGSEAIMLFGQNEIEEKIETFPSGSYLLDNALGIGGYPKGRIIEIYGPESSGKTTLCLHAIAEIQKQGGIAAYIDAEHSLDPNYARNLGINLDNLILSQPDSGEQALQIVDILAKSGSIDLIVVDSVAALVPEVELNGTMSDSSVGALARLMAKGLRKITGNLNKNKTAIIFVNQIREKIGVLFGNPETTTGGRAIKFFSSVRIEVRKTTSIVEGKDITGNEVKIKIVKNKLAAPYKSFTTEIIFSKGIDKLNELINLATEANVIEKKGA", "aa_hexdigest": "60b1b70bcf4cd495875ccf9d0878ed77", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XG20", "query_cov": 1.0, "subject_cov": 0.8651026392961877, "identity": 1.0, "score": 558.0, "evalue": 8.98e-200, "valid": true, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_Q07809", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_Q07809", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q07809" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "EFPKPENHBP_1316", "locus": "GCA000186005_01303" }, { "type": "cds", "sequence": "contig_1", "start": 716020, "stop": 716187, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_C4XG21" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTCCAAATAGAAGATGTTAGAAACAATTATAAAAACTTTTTAGGCTTGTTTAGCGAAGTAAGACTAAGAGTTAGATTATGCAGAAAAGAAAACATGCATTTTAGTGATGAATTACAAATAGATGCTTGTATCCATGAATGA", "aa": "MIKIAELLKVQIEDVRNNYKNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHE", "aa_hexdigest": "4878c4ad8a954fbe475a1c7480f64bd2", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.8909999999999999, "score": 102.0, "evalue": 1.26e-27, "valid": false, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 715921, "stop": 716187, "observations": { "mutation": [ 715935, 716022 ], "stop": [ 715935, 716022 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG21", "query_cov": 1.0, "subject_cov": 0.625, "identity": 1.0, "score": 114.0, "evalue": 4.31e-32, "gene_start": 1, "gene_end": 55, "reference_start": 1, "reference_end": 55, "reference_sequence": "MIKIAELLKVQIEDVRNNYKNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHEWFDGTKNAYYHAIDVAIIIIYFFAFVFYIWFRY" }, "paralog": false, "identity": 0.9772727272727273, "score": 174.0, "evalue": 4.35e-58, "description": "Internal stop codon at 715935, 716022. Nonsense mutation around 715935, 716022.", "psc": { "uniref90_id": "UniRef90_C4XG21", "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG21", "UniRef:UniRef50_C4XG21" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6619.675899999998, "isoelectric_point": 6.719755744934083 }, "id": "EFPKPENHBP_1317", "locus": "GCA000186005_01304" }, { "type": "cds", "sequence": "contig_1", "start": 716748, "stop": 716978, "strand": "+", "frame": 3, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGA", "aa": "MTKENKTLNILFIGDIFSEAGIEIVEKHLPSLIKENKIDVVIAQSENVSGRKGLVKKDYLRLKKAGVNIFTIGNHV", "aa_hexdigest": "2a3296f3a3bd25315eab08a421cf3e34", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9078947368421053, "subject_cov": 1.0, "identity": 0.58, "score": 89.7, "evalue": 3.53e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "EFPKPENHBP_1318", "locus": "GCA000186005_01305" }, { "type": "cds", "sequence": "contig_1", "start": 717321, "stop": 717566, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "GTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAA", "aa": "MQTNDAHVLPKGTCYLTDAGMSGPFDCAIGANFDEVYQKMRYNKNVKFIPSKNKSQFNGAILKLNSDKSQNKIIPINIRQE", "aa_hexdigest": "9d9c00019db0b50b5a1ddd9eecb21784", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 73, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.5140250352118277e-18, "score": 63.27640914916992, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 9110.331299999996, "isoelectric_point": 9.230534934997557 }, "id": "EFPKPENHBP_1319", "locus": "GCA000186005_01306" }, { "type": "cds", "sequence": "contig_1", "start": 717725, "stop": 717910, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24", "UniRef:UniRef50_C4XG24", "UniRef:UniRef90_C4XG24" ], "nt": "GTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAA", "aa": "MILSGILYRYFFTIYYKICKDKKEIKIMKYPKSFVDFSNIDVDLEASNELFLMGPLVFLFY", "aa_hexdigest": "d7bfa93e70bb7dd942a257ab1b248e14", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C18E", "uniref100_id": "UniRef100_C4XG24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24" ] }, "ips": { "uniref100_id": "UniRef100_C4XG24", "uniref90_id": "UniRef90_C4XG24" }, "psc": { "uniref90_id": "UniRef90_C4XG24", "uniref50_id": "UniRef50_C4XG24" }, "pscc": { "uniref50_id": "UniRef50_C4XG24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG24" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7363.714, "isoelectric_point": 7.796629142761231 }, "id": "EFPKPENHBP_1320", "locus": "GCA000186005_01307" }, { "type": "cds", "sequence": "contig_1", "start": 718481, "stop": 719071, "strand": "-", "frame": 3, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGTCAGGTGAAGCAATTGATATGCCTAATAATTTAATTGCTGGTAAAGCAATGGATAATAGAGCTGGTGTTACAGTTATTGACTTTTTAGCAAACAATATTAAAGATTTAGATTTACCGAATGAAACATATATTGTTGGTACAGTACAAGAAGAAATTGGAACAAGAGGTGCTAAAACTTCAGTTTCAATTATTGAACCAGATGTTGCTTTTGCAATTGATACAGGAGCAAGTCATGATACGACAGGATGTATTAAAGGAACTCCAGTTTTAGGTAAAGGTGTAGCTCTTTTAATACAAGATGGTGCTGTTATTACAGATCCTAAATTAGTTAACATGTTAGTAAAAATTGCTGAAAAACACAAAATTCCAGCTTATAAATATATAGCCGAAGGTGGCGGTACTGATGGAGCTAGTTTACAATATGGTAAAGGTGGGGTTCCTACAATTACTCTAAGTTTACCTCAACGTTATTTACACTCACCAATAGGCGTTTGTTCATTAGTAGATATTCAAGCAACAATTGATTTAATTACAGAATTTGTCAAAGCTTTTGACAACAAAATGTTAAATGAATTAAAAGCTAAATAA", "aa": "MSGEAIDMPNNLIAGKAMDNRAGVTVIDFLANNIKDLDLPNETYIVGTVQEEIGTRGAKTSVSIIEPDVAFAIDTGASHDTTGCIKGTPVLGKGVALLIQDGAVITDPKLVNMLVKIAEKHKIPAYKYIAEGGGTDGASLQYGKGGVPTITLSLPQRYLHSPIGVCSLVDIQATIDLITEFVKAFDNKMLNELKAK", "aa_hexdigest": "aa14b09513a8db6338a005c2107b1327", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 1.0051020408163265, "subject_cov": 0.933649289099526, "identity": 0.5529999999999999, "score": 225.0, "evalue": 5.93e-72, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "EFPKPENHBP_1321", "locus": "GCA000186005_01308" }, { "type": "cds", "sequence": "contig_1", "start": 719099, "stop": 719251, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAACAAATAGAGACAATAAAGAAATTTTTGGTATTTTTGGTCATACATCAATTCATATTATGGAAGCTGAAAAACGTGCTAAAGTTCCTACAACAAAAGAAATGTTTGTTGACTGCGGATTCAATTCAAAAGAAGAAGCTGAAAAATGA", "aa": "MVTNRDNKEIFGIFGHTSIHIMEAEKRAKVPTTKEMFVDCGFNSKEEAEK", "aa_hexdigest": "f7e0d5ae347e5fe47023b9314bbd5913", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5745.502199999999, "isoelectric_point": 6.056331062316894 }, "id": "EFPKPENHBP_1322", "locus": "GCA000186005_01309" }, { "type": "cds", "sequence": "contig_1", "start": 719282, "stop": 719566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATATGATAAATTAGCAAAAAGATTAAAAGAATACATGGCTATTGAAGCTATGAGTAGATATGAAGAACCTGTTGTTGAAGCTTTAAAGAAGAATACAGCTTCAAAAAATTTTGAATATTCAAGAGATGGTTTTGGTTCTTTAATTATTAAAAATAAAACAAAAGTTAAAAATGCACCAAAAATTATGATTGCTGCACACATGGATGAAGTTGGTTATTTAGTTAGATCAATCGAACCAAATGGTAACTTATTAGTTTCAGTTGTTGGAGGTGTTTGA", "aa": "MAKYDKLAKRLKEYMAIEAMSRYEEPVVEALKKNTASKNFEYSRDGFGSLIIKNKTKVKNAPKIMIAAHMDEVGYLVRSIEPNGNLLVSVVGGV", "aa_hexdigest": "d833bab3ea9363b9634ee1fce8aed2cc", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10508.202299999999, "isoelectric_point": 9.486925697326662 }, "id": "EFPKPENHBP_1323", "locus": "GCA000186005_01310" }, { "type": "cds", "sequence": "contig_1", "start": 720352, "stop": 720570, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGA", "aa": "MKKTVKNKKNNEALIIKNRLVEAANMNQNEIYEKYNSSNLGITKNEIIEENKENYGVNKLSKKKRGGVLKRI", "aa_hexdigest": "f4d0319613161825ce66d2a0340f6dd1", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 48, "aa_cov": 0.6527777777777778, "hmm_cov": 0.9791666666666666, "evalue": 8.14744182326659e-07, "score": 24.98471450805664, "start": 25, "stop": 71 } ], "seq_stats": { "molecular_weight": 8336.564999999995, "isoelectric_point": 9.946262931823732 }, "id": "EFPKPENHBP_1324", "locus": "GCA000186005_01311" }, { "type": "cds", "sequence": "contig_1", "start": 720513, "stop": 720689, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACAGTTCAAGGTTCGGATTTTTCACCTTTAGTTAAAGGAAGTATTATGTCAGTAATTAATGAAACTATTGAAAGTAAAATCAAGATCAATGAAAAAGGATTGAAGAAGCTATCTCATATTCTTTTTAATACTCCGCCTCTTTTCTTTTTGCTGAGTTTATTAACTCCATAA", "aa": "MDTVQGSDFSPLVKGSIMSVINETIESKIKINEKGLKKLSHILFNTPPLFFLLSLLTP", "aa_hexdigest": "903eb4c9a151afbd9cd8e2011158b000", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6431.557799999997, "isoelectric_point": 8.121356391906737 }, "id": "EFPKPENHBP_1325", "locus": "GCA000186005_01312" }, { "type": "cds", "sequence": "contig_1", "start": 720700, "stop": 721173, "strand": "+", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGA", "aa": "MVIISGILHFVEETRSSASAEKLVKMIQTTTRVERNGVAYEIPLEEVVVGDIVVLAAGDIIPADVRILSATDLFVSQSQLTGESDSIEKFPNLVPNYDYDNVTDYHNLAFMGSNVISGSAKAMVVVTGDQTYIGQVAQKINEKAPKTDFEKGIKSIS", "aa_hexdigest": "fb3ae051800ebd2e30134e9a80545abf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 1.0063694267515924, "subject_cov": 0.8272251308900523, "identity": 0.551, "score": 156.0, "evalue": 1.46e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "EFPKPENHBP_1326", "locus": "GCA000186005_01313" }, { "type": "cds", "sequence": "contig_1", "start": 721189, "stop": 722577, "strand": "+", "frame": 1, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "db_xrefs": [ "EC:7.2.2.14", "GO:0015444", "GO:0015693", "GO:0016020", "KEGG:K01531", "SO:0001217", "UniRef:UniRef50_A0A502M658", "UniRef:UniRef90_C4XG28" ], "nt": "ATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGTGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAAAGTTTTAAAATATGGATTCTTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAATGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGTAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTATGATTTTGCTTGTGGAACAATTCCTTGA", "aa": "MLVAVPLVLIITGTRASIKGEHEKWVDALLFAISVAIGLTPEMLPMIITSTLAKGAMSMSKKQTIVKSLNSIQNFGAMDIFCTDKTGTLTLDQVVLERHLDVRGLENIKVLKYGFLNSYYQTGLKNLLDISIINKTDELSDVEMELRSLEEIYKKVDEIPFDFIRKRMSVVVKSIKSDKTQLITKGAVEEILSICSKLEYKGEVVDLDEKMIQKVLKQVDKLNDEGMRVIAMARKSNPSQVGKFGVADEKDMILIGYLAFLDPPKESTKSAVENLHNLGVEVKILTGDNARVTRAICAQVGIPTDKIMLGKDLMELSDEELQKVANEYNIYAKLSPDQKARVITALRANGHVVGYMGDGINDAPAMKVADVSISVDTAVDIAKESANIILLEKDLNVLATGITEGRKTYANMNKYVKMTVSSNFGNIISMILASILIPFVPLMAIQVLFLNLIYDFACGTIP", "aa_hexdigest": "6480c29640fac73003734c8234ee5734", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0009C0D368", "query_cov": 0.9523809523809523, "subject_cov": 0.9128630705394191, "identity": 0.525, "score": 447.0, "evalue": 4.33e-151, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X2TA75", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "genes": [ "mgtA" ], "truncated": "both", "pseudogene": { "start": 720889, "stop": 722880, "observations": { "mutation": [ 721171, 722575, 722617, 722770, 722779, 722797 ], "stop": [ 721171, 722575, 722617, 722770, 722779, 722797 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG28", "query_cov": 1.0, "subject_cov": 0.5032679738562091, "identity": 1.0, "score": 868.0, "evalue": 1.57e-310, "gene_start": 1, "gene_end": 462, "reference_start": 294, "reference_end": 755, "reference_sequence": "MINNIYYLNGREVIMKKTVKNKKNNEALIIKNRLVEAANMNQNEIYEKYNSSNLGITKNEIIEENKENYGVNKLSKKKRGGVLKRIWDSFFNPFSLILILLSIVSLITDIILPLTKGEKSEPWTVSIILTMVIISGILHFVEETRSSASAEKLVKMIQTTTRVERNGVAYEIPLEEVVVGDIVVLAAGDIIPADVRILSATDLFVSQSQLTGESDSIEKFPNLVPNYDYDNVTDYHNLAFMGSNVISGSAKAMVVVTGDQTYIGQVAQKINEKAPKTDFEKGIKSISWLLIKIMLVAVPLVLIITGTRASIKGEHEKWVDALLFAISVAIGLTPEMLPMIITSTLAKGAMSMSKKQTIVKSLNSIQNFGAMDIFCTDKTGTLTLDQVVLERHLDVRGLENIKVLKYGFLNSYYQTGLKNLLDISIINKTDELSDVEMELRSLEEIYKKVDEIPFDFIRKRMSVVVKSIKSDKTQLITKGAVEEILSICSKLEYKGEVVDLDEKMIQKVLKQVDKLNDEGMRVIAMARKSNPSQVGKFGVADEKDMILIGYLAFLDPPKESTKSAVENLHNLGVEVKILTGDNARVTRAICAQVGIPTDKIMLGKDLMELSDEELQKVANEYNIYAKLSPDQKARVITALRANGHVVGYMGDGINDAPAMKVADVSISVDTAVDIAKESANIILLEKDLNVLATGITEGRKTYANMNKYVKMTVSSNFGNIISMILASILIPFVPLMAIQVLFLNLIYDFACGTIPWDKVDKELITKPRKWNAKSILRFMLWFGPVSSIVDILSFILLYYVFIPYAHPSVAKDSEQFKMLFWTGWFIISMWTQSLVIHFIRTEKAAFIQSKPAFILLFFSIFGSVLITITPYIPGLNTALKVTQLNPWFYGFLVGSIALYISLVLIVKKIYIIKYNELL" }, "paralog": false, "identity": 0.9909502262443439, "score": 1252.0, "evalue": 0.0, "description": "Internal stop codon at 721171, 722575, 722617, 722770, 722779, 722797. Nonsense mutation around 721171, 722575, 722617, 722770, 722779, 722797.", "psc": { "uniref90_id": "UniRef90_C4XG28", "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A502M658", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG28", "EC:7.2.2.14", "UniRef:UniRef50_A0A502M658", "KEGG:K01531", "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A502M658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M658" ], "product": "Magnesium-transporting ATPase, P-type 1" } }, "id": "EFPKPENHBP_1327", "locus": "GCA000186005_01314" }, { "type": "cds", "sequence": "contig_1", "start": 723569, "stop": 723730, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCACTTGATCCTAAATATATTGACTATGTGATAGTTCATGAATTATGCCACTACTTTCATATGGATCATTCTGAAGAATTTTATAAATTGGGTGAATTTTATTATCCTAATTTTAAAGAATATTCAGACACAATTAATAAACATAAAATTAATTTTTGA", "aa": "MALDPKYIDYVIVHELCHYFHMDHSEEFYKLGEFYYPNFKEYSDTINKHKINF", "aa_hexdigest": "de568364f38ef790125d47555de67207", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6624.400299999998, "isoelectric_point": 5.582237052917479 }, "id": "EFPKPENHBP_1328", "locus": "GCA000186005_01315" }, { "type": "cds", "sequence": "contig_1", "start": 724019, "stop": 724336, "strand": "-", "frame": 3, "gene": null, "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30", "UniRef:UniRef90_C4XG30" ], "nt": "ATGACTCAAAGAAAAAATAATTTAGTTTTAATTAAACACATCATAATTGATGATGTTAGTTATCCAGTTTATTCAAAAGTTAATGTCCGTTGTGAAAATATAATCGTTAGATTTAATAGTGACCAAAAAGTATTTGAGGTCACTAGTTTTAGACATATTAGTGAGGCTGATTATAATTTTCATAATTTAGATAGACTACTTATTAAAGCTTTTAAAAAAGCAAATAAAAGATTAAATAATGCTATAGTTTTAAATTTAACTAGCGGAGTTGAAGAACACTACATTAGTTTTTTTGGCAAAGAAACATTATTAAAATGA", "aa": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFHNLDRLLIKAFKKANKRLNNAIVLNLTSGVEEHYISFFGKETLLK", "aa_hexdigest": "ff7b35607f9d980cca6abd0657a87b27", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 723716, "stop": 724336, "observations": { "mutation": [ 723790, 724021 ], "stop": [ 723790, 724021 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG30", "query_cov": 1.0, "subject_cov": 0.41015625, "identity": 1.0, "score": 207.0, "evalue": 9.16e-66, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFHNLDRLLIKAFKKANKRLNNAIVLNLTSGVEEHYISFFGKETLLKWSFHYRDTSDGVYDKENNILTIRCSIKCASDQNKRLKVFEKTLTKFLEDYISKKQLVYSALMQINNPYFYVRMKKSAWGTHHKKYNVFSKLSYSLFLVALDPKYIDYVIVHELCHYFHMDHSEEFYKLGEFYYPNFKEYSDTINKHKINFW" }, "paralog": false, "identity": 0.9902912621359223, "score": 405.0, "evalue": 2.76e-146, "description": "Internal stop codon at 723790, 724021. Nonsense mutation around 723790, 724021.", "psc": { "uniref90_id": "UniRef90_C4XG30", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG30", "UniRef:UniRef50_C4XG30" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30" ], "product": "YgjP-like metallopeptidase domain-containing protein" } }, "id": "EFPKPENHBP_1329", "locus": "GCA000186005_01316" }, { "type": "cds", "sequence": "contig_1", "start": 724317, "stop": 724454, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAGATCGAAACTTAAATAAAGATGAATTAATGTTAATCAATGAATCAGTGAGTTCAAATAGAAGTGCTTCACTTAAAGACATTAAACGTTTTGGAAAGATCTTAAACAAAAATGACTCAAAGAAAAAATAA", "aa": "MSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK", "aa_hexdigest": "8e005d109165793d4f611c438c2825e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5195.8859, "isoelectric_point": 9.77445468902588 }, "id": "EFPKPENHBP_1330", "locus": "GCA000186005_01317" }, { "type": "cds", "sequence": "contig_1", "start": 724512, "stop": 725018, "strand": "-", "frame": 2, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31", "UniRef:UniRef90_C4XG31" ], "nt": "ATGATTAAACCAAGTAGAGAAATGATAATTGGACTATCAGTTGGAATTCCTTTAACACTATTAATTATTGTGCTAGTGTTAACTTTTTTAATTATCAAACTTATTAAAAGCAAACGCAATAATTCAATTGGTTTTCGTTTTGAAGCAAAAATTAGAAATAAACTTCTAACTATTGCTAAAGATAAAAACTACAAATACTTAAATGGTGGTTTATTTAAATATGGACAAAGTCAAATATTTGAACTTGATGGTATTTTAATTAGTAATAAGGCAGTTTTTATTATTGAAGTTAAATATTATGTTGGCCATCTTAGTGGTGAAGCTACTAATGCTGAATTAGAGTTAAGAAGCAAAAGCAATAAAGTTTTGAAATTTAAAAATCCGCTTATTCAAAACTTTAAACACATTCAACACTTTTACAAAATGTGTGGCTTTAACTTCCCAGTATTTTCATTGGTAATCTTGCCTTCAAATACTACTTTTGATATAGCTAACTTAGAATCATGA", "aa": "MIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLES", "aa_hexdigest": "b7fec0ca1287f650dd67e23f100062f5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 724317, "stop": 725051, "observations": { "mutation": [ 724514 ], "stop": [ 724514 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG31", "query_cov": 1.0, "subject_cov": 0.6885245901639344, "identity": 1.0, "score": 319.0, "evalue": 4.55e-109, "gene_start": 1, "gene_end": 168, "reference_start": 12, "reference_end": 179, "reference_sequence": "MQILILNIIINMIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLESWALVTTEDQVANLLEDVAKDMSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK" }, "paralog": false, "identity": 0.9918032786885246, "score": 465.0, "evalue": 5.13e-169, "description": "Internal stop codon at 724514. Nonsense mutation around 724514.", "psc": { "uniref90_id": "UniRef90_C4XG31", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG31", "UniRef:UniRef50_C4XG31" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31" ], "product": "NERD domain-containing protein" } }, "id": "EFPKPENHBP_1331", "locus": "GCA000186005_01318" }, { "type": "cds", "sequence": "contig_1", "start": 725097, "stop": 725285, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAACAGCTTTTTGA", "aa": "MSAKKIISILGSVNKNSLSNKINNIVSSKLFKKYPNSEITLLDLANSPFSELMLNGHDTTAF", "aa_hexdigest": "6b19def8555b70166c7db5f108cdfd31", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6798.7907, "isoelectric_point": 9.523801612854005 }, "id": "EFPKPENHBP_1332", "locus": "GCA000186005_01319" }, { "type": "cds", "sequence": "contig_1", "start": 725355, "stop": 725519, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGA", "aa": "MINFNYSALVKNFIDAICIADHSFSYKYSKKNGSVGLLNKMKVIIIGTQGAPKG", "aa_hexdigest": "261b72bc83ebc312a1971da398a55dd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9622641509433962, "evalue": 5.253104158263262e-10, "score": 35.646446228027344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5909.8963, "isoelectric_point": 9.66846866607666 }, "id": "EFPKPENHBP_1333", "locus": "GCA000186005_01320" }, { "type": "cds", "sequence": "contig_1", "start": 725544, "stop": 725687, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAAGGAACATTTAACTTTTTAGGAGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATGCTTTAATTTAA", "aa": "MLEGTFNFLGAKEVKTLLVAGTKVTPLKDMSQEEIIKQYQKEIDALI", "aa_hexdigest": "553991972b2c020f8bed4c015ac62b2c", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.1615999999985, "isoelectric_point": 5.144690513610839 }, "id": "EFPKPENHBP_1334", "locus": "GCA000186005_01321" }, { "type": "cds", "sequence": "contig_1", "start": 725764, "stop": 725922, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "ATGTCAATTGAACCTAAAATAATAATTAATGAATCAGTTGAAATAACTAAATATTATTTTGAAGATGAAAAAAACTTTTACAAAATGGTTAATGCAATTCTAGAAAAAGTTTATAAATTCTTTGTTATCAATGAAGTTATTTTAAAAAAACAAGATTAA", "aa": "MSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKQD", "aa_hexdigest": "dc21ac322b43ef772389b9ca666207f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 38, "aa_cov": 0.7307692307692307, "hmm_cov": 0.9473684210526315, "evalue": 2.015073176429422e-07, "score": 27.785888671875, "start": 2, "stop": 39 } ], "seq_stats": { "molecular_weight": 6336.368700000002, "isoelectric_point": 5.3466394424438475 }, "id": "EFPKPENHBP_1335", "locus": "GCA000186005_01322" }, { "type": "cds", "sequence": "contig_1", "start": 725983, "stop": 726183, "strand": "-", "frame": 1, "gene": null, "product": "NusB/RsmB/TIM44 domain-containing protein", "db_xrefs": [ "KEGG:K03625", "SO:0001217", "UniRef:UniRef50_C4XG33", "UniRef:UniRef90_C4XG33" ], "nt": "ATGCAAAACAGACGTCAATTTCGTTTAGGTATAATTAATGTTCTTTACCGTTATGAATTAATGGATCAAAAAATCAATGTCAATGAATTATTTGATTCAGAAAGTAACTTAAGTAATGAACAATTTTCGCATCTTGAAAAGATAGCAAAAAACTATGATTTTTATAAAAAAGCAGTCAGTCAATTTTTAAAAGAAGGTTGA", "aa": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEG", "aa_hexdigest": "7d736dd278c923cfe77172f929eee637", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 725764, "stop": 726183, "observations": { "mutation": [ 725979, 725985 ], "stop": [ 725979, 725985 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG33", "query_cov": 1.0, "subject_cov": 0.4748201438848921, "identity": 1.0, "score": 129.0, "evalue": 2.5e-37, "gene_start": 1, "gene_end": 66, "reference_start": 1, "reference_end": 66, "reference_sequence": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEGWSWERISPLIRAILINGAHELMSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKQD" }, "paralog": false, "identity": 0.9856115107913669, "score": 267.0, "evalue": 6.19e-94, "description": "Internal stop codon at 725979, 725985. Nonsense mutation around 725979, 725985.", "psc": { "uniref90_id": "UniRef90_C4XG33", "product": "NusB/RsmB/TIM44 domain-containing protein", "uniref50_id": "UniRef50_C4XG33", "kegg_orthology_id": "K03625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG33", "UniRef:UniRef50_C4XG33", "KEGG:K03625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG33" ], "product": "NusB/RsmB/TIM44 domain-containing protein" } }, "id": "EFPKPENHBP_1336", "locus": "GCA000186005_01323" }, { "type": "cds", "sequence": "contig_1", "start": 726257, "stop": 726667, "strand": "-", "frame": 3, "gene": null, "product": "DUF1934 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354794.1", "SO:0001217", "UniParc:UPI0001A8C198", "UniRef:UniRef100_C4XG34", "UniRef:UniRef50_C4XG34", "UniRef:UniRef90_C4XG34" ], "nt": "ATGAAAATAAGATTTACTTCAATTCAAGATCATAAAATTAAAAACCAAGAACCTAAAATAGTTTCTTTTGAAGCTGATGCAGAATATGATTTATTTTTAGATGATGAAGATCCAAAGCAATTACAATATCATACATATCATTTTAAAGAGCCTTCAATTGGTGAATCTAACCGTATAGAAATCAATAGTGAAAGAGTAAATATTTTTACAGGAATGTCAACGTTGACAATGTTAAAAGGTCAAAAAGCTCCTAACTCTTTTGTTAAAGTAGATGGCACACAATTCTTTTTAAAACCATTTTTACATGAAGTTGTTATTGAAGGCAATATTAAAAAATTCAAATATGAATTATTTGGCCCTAAAGATGATTTAATTGGTGAATTCAATGTTTCGATTGAAGAATTAGAATAA", "aa": "MKIRFTSIQDHKIKNQEPKIVSFEADAEYDLFLDDEDPKQLQYHTYHFKEPSIGESNRIEINSERVNIFTGMSTLTMLKGQKAPNSFVKVDGTQFFLKPFLHEVVIEGNIKKFKYELFGPKDDLIGEFNVSIEELE", "aa_hexdigest": "2174371c5bb31c858753072833bd3158", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C198", "ncbi_nrp_id": "WP_013354794.1", "uniref100_id": "UniRef100_C4XG34", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C198", "RefSeq:WP_013354794.1", "UniRef:UniRef100_C4XG34" ] }, "ips": { "uniref100_id": "UniRef100_C4XG34", "uniref90_id": "UniRef90_C4XG34" }, "psc": { "uniref90_id": "UniRef90_C4XG34", "product": "DUF1934 domain-containing protein", "uniref50_id": "UniRef50_C4XG34" }, "pscc": { "uniref50_id": "UniRef50_C4XG34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG34" ], "product": "DUF1934 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1337", "locus": "GCA000186005_01324" }, { "type": "cds", "sequence": "contig_1", "start": 726664, "stop": 726942, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "ATGCAGCCATATTTAAAACCAACAAATAAAAAACCAGTTGTCGAAGCTGAAAATCATGATGTAATTTTAACTTTTAAAGATTTATTAAATAAAAACACTTTTACTATTGAAAATATTCAAGCTGCAATTGACCAAACAAAAGAAATTACAGGTAAAAAAGGCAAAAATCTATTTATGCCAATACGTCTAGCAACCACTTACATTGAACATGGTCCTGAATTAGCTAAAGCAATTTATTTATATGGCGAAGACTTAATTAAAGAAAGGTTAGCAAAATGA", "aa": "MQPYLKPTNKKPVVEAENHDVILTFKDLLNKNTFTIENIQAAIDQTKEITGKKGKNLFMPIRLATTYIEHGPELAKAIYLYGEDLIKERLAK", "aa_hexdigest": "716b3e44b08ce9adaafe89facb90e8e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 79, "aa_cov": 0.8478260869565217, "hmm_cov": 1.0, "evalue": 5.920814599547222e-10, "score": 36.024383544921875, "start": 15, "stop": 92 } ], "seq_stats": { "molecular_weight": 10529.14899999999, "isoelectric_point": 8.831411170959473 }, "id": "EFPKPENHBP_1338", "locus": "GCA000186005_01325" }, { "type": "cds", "sequence": "contig_1", "start": 727192, "stop": 727308, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0", "UniRef:UniRef90_UPI0021FF3334" ], "nt": "ATGGAAGGTAAAAAACTTTCAAAACGTGATACTAGTTTAAAACAATTTATTGAAGATTATCGTAATGAAGGCTACCATCCTGAAGGTGTATTTAACTTTTTAAGTTTGCTTGGTTGA", "aa": "MEGKKLSKRDTSLKQFIEDYRNEGYHPEGVFNFLSLLG", "aa_hexdigest": "0fa7d902c7d175366f672234bafc8822", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 727144, "stop": 727308, "observations": { "mutation": [ 727194 ], "stop": [ 727194 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF3334", "query_cov": 1.0, "subject_cov": 0.6909090909090909, "identity": 0.868, "score": 73.6, "evalue": 1.28e-16, "gene_start": 1, "gene_end": 38, "reference_start": 1, "reference_end": 38, "reference_sequence": "MEGKKLSKRDTSLKQFIEDYKNEGYLPEAIFNFLTLLGWTASDASELMSKDEIIS" }, "paralog": false, "identity": 0.7407407407407407, "score": 83.2, "evalue": 2.92e-23, "description": "Internal stop codon at 727194. Nonsense mutation around 727194.", "psc": { "uniref90_id": "UniRef90_UPI0021FF3334", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF3334", "UniRef:UniRef50_A0A3B0PLI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0" ], "product": "Glutamate--tRNA ligase" } }, "id": "EFPKPENHBP_1339", "locus": "GCA000186005_01326" }, { "type": "cds", "sequence": "contig_1", "start": 727858, "stop": 728049, "strand": "-", "frame": 1, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "nt": "ATGAAAAAAATAAGAACCAGATATGCGCCAAGTCCAACAGGTTACTTACACATTGGAGGTGCTAGAACAGCATTATTTTGCTATTTATATGCTAAGCACTTTAAAGGTGATTTTGTTTTCAGATTAGAAGACACTGATGTTAAAAGAAATGTAGCTGATGGTGAAAGAAGTCAATTAGAAAACTTAGCTTGA", "aa": "MKKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFKGDFVFRLEDTDVKRNVADGERSQLENLA", "aa_hexdigest": "71b6c1c2e80d80f5a82ea70deef88add", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0037F98A17", "query_cov": 0.9523809523809523, "subject_cov": 0.8571428571428571, "identity": 0.5670000000000001, "score": 79.3, "evalue": 2.79e-18, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2N2SDT7" }, "pscc": { "uniref50_id": "UniRef50_A0A2N2SDT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "EFPKPENHBP_1340", "locus": "GCA000186005_01327" }, { "type": "cds", "sequence": "contig_1", "start": 728336, "stop": 729205, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013526998.1", "SO:0001217", "UniParc:UPI0001F59BF5", "UniRef:UniRef100_C4XG36", "UniRef:UniRef50_C4XG36", "UniRef:UniRef90_C4XG36" ], "nt": "ATGAAATTAAAAAAAATATTTAAATTTGGAGCATTGTTAACTTCACCAATCATGTTAGTTCCTATTGTTTCATGTGGCAAAAATCAAAAAGATGTTAATTCAACAAAATTCTTTGAATTTGAAAGTCCGATGCCTAAAAACAATAAACCAAATAATGAAATAAGTGAAAAAGAAAAATTTCAAAATTGTCAAAACGCATATGAAAAAATTATCCAATTAGCATCATTTAAAAATGCGCTTCAAAGTGATTTTGATGCAAATAACGTAAGTGAATATGGACCTGAAAAAATTTTAGATTTTAAAGCTAAGGACGGTCTTGCATTACTTAAAGCTACATATTTTAATAAAGAATCTGAATATGAAAAAATAGTTGAAGTTTATAAATCAATTAATCCTACAGGAAACTTTAATGATTATTTAACTAATGAATATAAAAAGATGACAAGTGCAATTAATATTTATCTTAAAGCTGAAGATAATCAAAGTGCAAAAAATATTAAAGAAGAATTAAATGATCCTAAAAAAGTTAAAGAAATAAATAACAAGGTTAATAATATAAAAGAAACAATTAACAAATTGATTGATAAATTAAATGAAAATCCTGAAATCAAAAGTAAAGTTGATGAATCTTTGAAAAAACAAAACTTATCAATAGAAAAAATTAAAGAAAAAATCAGTGGTTATGATAATAATGAAATTGCAAAAGGAATTTTTCTTGGACTTAATGGAATAATAAAGAATAAAGACGAACTATTTAAAAATCCTAATTTATCAAAATTTGCTCCGATTATTCCAAACAGACCTTTTGATAATTTGAATGAATCATTAGATGTAATTAGCAATGTTTTGTCGACTGCAAATCTATTTTAA", "aa": "MKLKKIFKFGALLTSPIMLVPIVSCGKNQKDVNSTKFFEFESPMPKNNKPNNEISEKEKFQNCQNAYEKIIQLASFKNALQSDFDANNVSEYGPEKILDFKAKDGLALLKATYFNKESEYEKIVEVYKSINPTGNFNDYLTNEYKKMTSAINIYLKAEDNQSAKNIKEELNDPKKVKEINNKVNNIKETINKLIDKLNENPEIKSKVDESLKKQNLSIEKIKEKISGYDNNEIAKGIFLGLNGIIKNKDELFKNPNLSKFAPIIPNRPFDNLNESLDVISNVLSTANLF", "aa_hexdigest": "a2d0df164650869171eab2137048e069", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001F59BF5", "ncbi_nrp_id": "WP_013526998.1", "uniref100_id": "UniRef100_C4XG36", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BF5", "RefSeq:WP_013526998.1", "UniRef:UniRef100_C4XG36" ] }, "ips": { "uniref100_id": "UniRef100_C4XG36", "uniref90_id": "UniRef90_C4XG36" }, "psc": { "uniref90_id": "UniRef90_C4XG36", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XG36" }, "pscc": { "uniref50_id": "UniRef50_C4XG36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG36" ], "product": "Lipoprotein" }, "genes": [], "id": "EFPKPENHBP_1341", "locus": "GCA000186005_01328" }, { "type": "cds", "sequence": "contig_1", "start": 729411, "stop": 729518, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGAAATATTATTTAAAAGATGATTCTAAATTAAGTTATTTAGATTTAATTATGAATACAGCTAACAATAATGATTATTATGTAGAAATGGTTCAAGCTTGA", "aa": "MLKKYYLKDDSKLSYLDLIMNTANNNDYYVEMVQA", "aa_hexdigest": "5c74b3d0f0f832c9857cf9ed2202a669", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4208.783600000002, "isoelectric_point": 4.777908515930175 }, "id": "EFPKPENHBP_1342", "locus": "GCA000186005_01329" }, { "type": "cds", "sequence": "contig_1", "start": 729795, "stop": 729956, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38", "UniRef:UniRef90_C4XG38" ], "nt": "ATGAATGAATTAAGATGTAGAAAAGAAGAAAAACTTTATTATAAATTAATACCTTATATTGATCAAAAATATAAAGAATTTTGCTTGCAAATTTATAAAATTGAAAGTGCAAAATTATTAGGAGTTAGAATTCCAATACTTAAAGATTTAGCTAAAAAATGA", "aa": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLQIYKIESAKLLGVRIPILKDLAKK", "aa_hexdigest": "99f63e159400816f497060608c1bab0a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 729674, "stop": 729956, "observations": { "insertion": [ 729728 ], "mutation": [ 729758, 729797 ], "stop": [ 729758, 729797 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG38", "query_cov": 1.0, "subject_cov": 0.5698924731182796, "identity": 1.0, "score": 107.0, "evalue": 3.61e-29, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLQIYKIESAKLLGVRIPILKDLAKKWYKEIPTKVSEFMWNPYHYLFEEIYFMVFLLKKLMIIKSK" }, "paralog": false, "identity": 0.978494623655914, "score": 177.0, "evalue": 2.06e-59, "description": "Internal stop codon at 729758, 729797. Frameshift due to insertion around 729728. Nonsense mutation around 729758, 729797.", "psc": { "uniref90_id": "UniRef90_C4XG38", "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG38", "UniRef:UniRef50_C4XG38" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6478.775, "isoelectric_point": 9.481768226623537 }, "id": "EFPKPENHBP_1343", "locus": "GCA000186005_01330" }, { "type": "cds", "sequence": "contig_1", "start": 730462, "stop": 730755, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTGTTATGATTGGTGGTATGGTTCCTCCTCTTGTAATCGCTATTGCAGCTGACTTATTCCCTCAAAAATTTACTGCTAAAGAAAGAAAAGATTCAAAAGTTAACTACTTAATGGGCATGGCATTTATTACTGAAGGTACAATTCCTTATGCAGCTAGTGATCCCTTGAGAGTTATTGGTTCATCAATTTTTGCAAGTACATTAGCTGGTGGAATGGCAGCTGGATTAGGATGCTCAATTCCTGCTCCTCATGGCGGAATCTTTGTATTCGTTGTTGCACAAGGATGA", "aa": "MAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGTIPYAASDPLRVIGSSIFASTLAGGMAAGLGCSIPAPHGGIFVFVVAQG", "aa_hexdigest": "385df7906a33e218c1dbc36b538bcc55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9901.656399999994, "isoelectric_point": 7.90732135772705 }, "id": "EFPKPENHBP_1344", "locus": "GCA000186005_01331" }, { "type": "cds", "sequence": "contig_1", "start": 730759, "stop": 731025, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATCCAGTATTTGGTATCTTACTTATTGCTGTTATTATGTTCTTTGTAATTAATACACCACTTTCATATTTAAATTACTACATTCAACAAGGTTTAAACTATGTTGTTAAAAATAACACAATTTCAATTGGCTTTAAAATTGGAATGGGAGCTTTACTTGCTGGTATGATGGCAGTTGATATGGGTGGTCCAGTTAACAAAGTTGCATATACTATTGGTGTTGCTTCAGTAGATCCTGATGGAATCAATCAACCATGA", "aa": "MLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGALLAGMMAVDMGGPVNKVAYTIGVASVDPDGINQP", "aa_hexdigest": "658b1ea6237a8689bc7b0be39e4e3a29", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9474.214999999998, "isoelectric_point": 5.792711830139161 }, "id": "EFPKPENHBP_1345", "locus": "GCA000186005_01332" }, { "type": "cds", "sequence": "contig_1", "start": 731068, "stop": 731676, "strand": "-", "frame": 1, "gene": null, "product": "PTS EIIC type-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG39", "UniRef:UniRef90_C4XG39" ], "nt": "TTGACAAATGAAGATATTCAAAAAGCTTATGGTGTCATTGTTGCTGCCGATACAAATATTGAAATCACAAGATTCAATGGTAAAAGATTGCTTCAAACAACTGTAACAAAAGGAATTAAACAACCCAAAGAATTAATACAAGAAATAATAGATGAAAAAGTAGCTGTATATAAATCAGATTCTTCTTCAAATGAAAATACAAATCAAGAAAAATTAAGTTTTGGAAAACAAATATATAAGAATTTAATGAACGGTGTAAGCCATATGCTTCCATTTGTTATTGGTGGCGGTATTCTAATTGCTCTAGCCTTCTTATTTGACTCTTTTTTAGGAAAAGAAGCAGTAGGAAAAGATTTTGGACAATATACTCCATTTTCAAAATTCTTTAAAAATGCTGGTGGATTTGCTTTTGGCTTTATGATGTCAGTGTTTGCTGGTTTTATTGCACATTCAATTGCAGGAAGACCAGGCTTAGCAGTCGGTATGGTAGCTGGAGCCATGGCATCTAGTACACAGTTTGCGGTTTATGAGACTGGTTCTAATGCAGGCTTCTTAGGAGCCTTAATTGGTGGTTTCATTGCCGGATTCTTAGTATTAGGATTAAAATGA", "aa": "MTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLK", "aa_hexdigest": "2cd91a8a0e90cade06225c91a85c37f7", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 730765, "stop": 731676, "observations": { "mutation": [ 731058, 731070 ], "stop": [ 731058, 731070 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG39", "query_cov": 1.0, "subject_cov": 0.43817787418655096, "identity": 0.946, "score": 356.0, "evalue": 8.75e-120, "gene_start": 1, "gene_end": 202, "reference_start": 1, "reference_end": 202, "reference_sequence": "MTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLKWCFKWLPKSLDGLKPMLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGALLAGMMAVDMGGPVNKVAYTIGVASVDPDGINQPWIMAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGTIPYAASDPLRVIGSSIFASTLAGGMAAGLGCSIPAPHGGIFVFVVAQGWYWYLLALVVGAFAGAFILGALRKKVDPSELGRWKGIPIGNGVYLKRKVKQAQKVEK" }, "paralog": false, "identity": 0.9537953795379538, "score": 562.0, "evalue": 2.22e-203, "description": "Internal stop codon at 731058, 731070. Nonsense mutation around 731058, 731070.", "psc": { "uniref90_id": "UniRef90_C4XG39", "product": "PTS EIIC type-2 domain-containing protein", "uniref50_id": "UniRef50_C4XG39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG39", "UniRef:UniRef50_C4XG39" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG39" ], "product": "PTS EIIC type-2 domain-containing protein" } }, "id": "EFPKPENHBP_1346", "locus": "GCA000186005_01333" }, { "type": "cds", "sequence": "contig_1", "start": 731695, "stop": 732315, "strand": "-", "frame": 1, "gene": null, "product": "PTS system protein", "db_xrefs": [ "RefSeq:WP_013527001.1", "SO:0001217", "UniParc:UPI0001F59D3F", "UniRef:UniRef100_A0AB32XC71", "UniRef:UniRef50_A0A1I6IS36", "UniRef:UniRef90_A0AB32XC71" ], "nt": "GTGAAAATAAAAGAACTTCTAACTCTAAAAAATATTGATATTAATGCAAAAGCAAAAGATAAATTAGAAGCCATTGAATTAGCAGTTAATTTGATGGGTGATAACATTATAATTGACAAAAACGCCTATTTGACAGCGGTTTTGGAAAGAGAAAAACAAACAACAACTGGATTGGGCGATGAATTAGCATTGCCTCATGGACGCGGATCTTTCGTTAAGAAACCTCACTTAAGTGCACTTGTTTTAAAAGACGGAGTTGATTATCAATCTTTAGATAATAAACCTGTTAAATTGCTATTTCTAATTGCAGCTCCAGAAACAACAGATAGTGAAAACTTACACTTAGAAGTTTTGGCTAAATTGAGCAAAATGTTAGTTGATAAAGTATTTATTGAAAAATTAATTAATGCTAAAAGCAACAAAGAATTTCTAAAATTAATTGAAACAAAAGAACAAGAAGTTGATCAGAATGAATTTGAACAAACTAATAATGGTGAAAAATTTATAGTAGCTGTTACAGCTTGTCCAACTGGTATTGCTCATACATACATGGCTCAAGAATCTTTAGAAAATGCAGCTAAGGAAATGGGTTATCAAATCAAAGTTGAAACTCAAGGTTAA", "aa": "MKIKELLTLKNIDINAKAKDKLEAIELAVNLMGDNIIIDKNAYLTAVLEREKQTTTGLGDELALPHGRGSFVKKPHLSALVLKDGVDYQSLDNKPVKLLFLIAAPETTDSENLHLEVLAKLSKMLVDKVFIEKLINAKSNKEFLKLIETKEQEVDQNEFEQTNNGEKFIVAVTACPTGIAHTYMAQESLENAAKEMGYQIKVETQG", "aa_hexdigest": "75faa41b3cae68b5513e0370ab33114c", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F59D3F", "ncbi_nrp_id": "WP_013527001.1", "uniref100_id": "UniRef100_A0AB32XC71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D3F", "RefSeq:WP_013527001.1", "UniRef:UniRef100_A0AB32XC71" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC71", "uniref90_id": "UniRef90_A0AB32XC71" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC71", "product": "PTS system protein", "uniref50_id": "UniRef50_A0A1I6IS36" }, "pscc": { "uniref50_id": "UniRef50_A0A1I6IS36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I6IS36" ], "product": "PTS system, fructose-specific IIC component" }, "genes": [], "id": "EFPKPENHBP_1347", "locus": "GCA000186005_01334" }, { "type": "cds", "sequence": "contig_1", "start": 732312, "stop": 733226, "strand": "-", "frame": 2, "gene": "pfkB", "product": "1-phosphofructokinase", "db_xrefs": [ "EC:2.7.1.56", "GO:0008662", "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0", "UniRef:UniRef90_C4XG41" ], "nt": "ATGATTTACACAGTGACTTTTTCACCAGCTATTGACTATACAATGTCTTTAGAGGGAAAAATAAATATAGGAGATATTAATCGCTCCAAAAGTGAAAATTATACAGTTGGTGGTAAAGGAATTAATGTTTCAATACTATTAAATAATTTAGGAACTAAAAATGTAGCTTTAGGTTTTGTCGGTGGTTTTACTGGTCAATATATTATTAATTACTTAAAAGATAAAAAAGTTAAAACAGATTTTTGTAATATTGAAGGTAATTCAAGAATTAATGTAAAACTAAATGGCAAAGCTAAAGAAACAGCTATTAATGCTAATGGACCTAAAATCAGTGATCAAAATTTAAAACAATTATTAGATAAGATTGCTTTTAATATTAAAGATAATGATACTTTAATTTTGGCTGGCAATGTTCCAAAAAATGTTGACACAAATGTTTATGAATTAATTTTAGAAAAACTCAAAAACAAAAGAATTTATATAATAGTAGATTCAACTAAACAATTTCTACTTAATACATTAAAATACAAACCATTTTTGATTAAACCTAATTTAGAAGAACTTGAAGAATTATTTGATGAAAAAGTTAAAACTAAAGAAGACATAAAAAGTCATGTTGAAAAACTTAAAAAATTAGGTGCCAGAAATGTGTTAGTGTCTTGTGGCGCTGAAGGAGCTTATTTATTTAGTGAAGATAATCAAACCTTTTATAGTGCTACATATAAAGGAAAAGTTGTAAGTACAGTTGGTTCAGGAGATAGCATGATTGCTGGATTTATTGATAATTATTTAAAAAATAAAGATTATAAACAAGCTTTAGAATTCTCAATAGCTTGTGGAGCAGCAACTGCTTTTGATAAAGAAATAGCTAATAAAAGTGCAATATTAGAATTACTTGAAAGGGGTCAAAAGTGA", "aa": "MIYTVTFSPAIDYTMSLEGKINIGDINRSKSENYTVGGKGINVSILLNNLGTKNVALGFVGGFTGQYIINYLKDKKVKTDFCNIEGNSRINVKLNGKAKETAINANGPKISDQNLKQLLDKIAFNIKDNDTLILAGNVPKNVDTNVYELILEKLKNKRIYIIVDSTKQFLLNTLKYKPFLIKPNLEELEELFDEKVKTKEDIKSHVEKLKKLGARNVLVSCGAEGAYLFSEDNQTFYSATYKGKVVSTVGSGDSMIAGFIDNYLKNKDYKQALEFSIACGAATAFDKEIANKSAILELLERGQK", "aa_hexdigest": "e5ee5bd9bfd88c9c6a56e7cc75693dae", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_C4XG41", "query_cov": 1.0, "subject_cov": 0.9934640522875817, "identity": 1.0, "score": 583.0, "evalue": 4.41e-210, "valid": true, "gene": "pfkB", "product": "1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_A0A3P8KBW0", "go_ids": [ "GO:0008662" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KBW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0" ], "product": "1-phosphofructokinase" }, "genes": [ "pfkB" ], "id": "EFPKPENHBP_1348", "locus": "GCA000186005_01335" }, { "type": "cds", "sequence": "contig_1", "start": 733369, "stop": 734112, "strand": "-", "frame": 1, "gene": null, "product": "HTH deoR-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527003.1", "SO:0001217", "UniParc:UPI0001F59D40", "UniRef:UniRef100_C4XG42", "UniRef:UniRef50_C4XG42", "UniRef:UniRef90_C4XG42" ], "nt": "ATGCTTAAAGAACAAAGACTAGATGTTATTTTAAAAGAAGTAAATTCAAAAGGTGCTGCTTCACTAAAAGAGCTTGCAAAATTAACTCAAGCAAGCATTTCTTCAATACGCGAAGATGTTGCTGAATTAGATAAACAAAAATTATTAAAAAGAGTTCATGGCGGAGCTTATTCATTAAATAATAATTCTTCAGCAAATGATGTTGAATATGAATCAAGAAGTAATATTGAATTAGATGCAAAAATCAAATTAGCTACCAAAGCAAATGAATTTGTACAAGATAACTTATTAATTTTTATTGACTCAGGAACAACAACTCATCAATTAGCTAAATTAATTAATAACAAGAATGTAACTATAGTTACGAATTCAATTAATGTTGCTACAGAATTAAAAAGTCATTCAAACGTTACAACTATTTTAATAGGTGGATTATTAAAAAGTAATACGTTTAATGTTGTTGGATCATTAGCTTTAGATAATTTGAAAAACTTTAATTTTGATATTGGATTTATTGGAGCAAATGGATATAGCAATAATCTAGGTTTTACAACACCTGAAATTAATGAAAGTTTATTTAAAAAAGAAGCAATTTTAAAATGCAAAAAAAGTTATATTTTGTTAGACAAAACCAAAATAAATTCTGAATTTAAATATACTTTTGCAACCAATAATAAAAACATCACATTGATAACTAATTACAAGAAAAAAGATCTAAATCTTGAGAATATCATAGAAGTATAA", "aa": "MLKEQRLDVILKEVNSKGAASLKELAKLTQASISSIREDVAELDKQKLLKRVHGGAYSLNNNSSANDVEYESRSNIELDAKIKLATKANEFVQDNLLIFIDSGTTTHQLAKLINNKNVTIVTNSINVATELKSHSNVTTILIGGLLKSNTFNVVGSLALDNLKNFNFDIGFIGANGYSNNLGFTTPEINESLFKKEAILKCKKSYILLDKTKINSEFKYTFATNNKNITLITNYKKKDLNLENIIEV", "aa_hexdigest": "e1131629919161a5cbf6664cef52baec", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59D40", "ncbi_nrp_id": "WP_013527003.1", "uniref100_id": "UniRef100_C4XG42", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D40", "RefSeq:WP_013527003.1", "UniRef:UniRef100_C4XG42" ] }, "ips": { "uniref100_id": "UniRef100_C4XG42", "uniref90_id": "UniRef90_C4XG42" }, "psc": { "uniref90_id": "UniRef90_C4XG42", "product": "HTH deoR-type domain-containing protein", "uniref50_id": "UniRef50_C4XG42" }, "pscc": { "uniref50_id": "UniRef50_C4XG42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG42" ], "product": "HTH deoR-type domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1349", "locus": "GCA000186005_01336" }, { "type": "cds", "sequence": "contig_1", "start": 734174, "stop": 734308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D41", "UniRef:UniRef100_A0AB32XCD5", "UniRef:UniRef50_A0AB32XCD5", "UniRef:UniRef90_A0AB32XCD5" ], "nt": "ATGAATAAAAATATAGATATGTCCTTAAACAATTTAGGGACATGTTTTTTTCTTATGTTCCAAATTGTAGATTTTAAAATGGGACATAATTTAATCCTTATGTCCCTAAAATTAAAAGAAATGTTTAATAATTAG", "aa": "MNKNIDMSLNNLGTCFFLMFQIVDFKMGHNLILMSLKLKEMFNN", "aa_hexdigest": "27e4ccb15e6ab8c8e5c6d05ec32e75af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59D41", "uniref100_id": "UniRef100_A0AB32XCD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D41", "UniRef:UniRef100_A0AB32XCD5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCD5", "uniref90_id": "UniRef90_A0AB32XCD5" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCD5", "uniref50_id": "UniRef50_A0AB32XCD5" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCD5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5200.235999999998, "isoelectric_point": 7.939620018005372 }, "id": "EFPKPENHBP_1350", "locus": "GCA000186005_01337" }, { "type": "cds", "sequence": "contig_1", "start": 734342, "stop": 734491, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTACAGGTCAAAAAACAATTCGTGATGTTATTGCATTTCCTAAGAATGCTAAAAACCAAGATGTTTTTACAGGAGCTCCAGGCAGTGTTGATAAAAAACAATTAGATGAATTATTCTTAGAAATTAAAAAAGAAGACAAATAA", "aa": "MIITGQKTIRDVIAFPKNAKNQDVFTGAPGSVDKKQLDELFLEIKKEDK", "aa_hexdigest": "07b496c0c7929d5adcab76461698f2aa", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5519.329399999999, "isoelectric_point": 8.034710884094238 }, "id": "EFPKPENHBP_1351", "locus": "GCA000186005_01338" }, { "type": "cds", "sequence": "contig_1", "start": 734561, "stop": 734713, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAAGGATGTTAGAGCGCTTTCATATGACTTAGTTTTAAATGGTTTTGAATTAGGTTCAGGTTCAGCAAGAATATTTGATAAAAAAACACAAAAGAAAATGTTTGATTTAATTGGTATGATTGAAAAAGAACAACAATCAAGATTTGATTGA", "aa": "MKDVRALSYDLVLNGFELGSGSARIFDKKTQKKMFDLIGMIEKEQQSRFD", "aa_hexdigest": "1cce22170a340808982aede1387a5288", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 47, "aa_cov": 0.94, "hmm_cov": 1.0, "evalue": 2.923090853697313e-11, "score": 39.26742935180664, "start": 3, "stop": 49 } ], "seq_stats": { "molecular_weight": 5785.628699999999, "isoelectric_point": 8.065204429626462 }, "id": "EFPKPENHBP_1352", "locus": "GCA000186005_01339" }, { "type": "cds", "sequence": "contig_1", "start": 734837, "stop": 735466, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTGTTCAGGTTTTGAAAAATATTATCAATTTGCAAGATGTTTCAGAGATGAAGATAGTAGAAAAGATCGTCAACCAGAATTTACTCAATTAGATATTGAAACTTCATTCTTAAATGTTGAAGACTTTCAAAAATACATTGAAAAATTATTCAAACACATTTTTAAATCAATAGGAATTAAAATCAAGACTCCATTTCCAAGAGTTAAATATTTTGATGCTTTAAGAGATTATGGAACAGATAAGCCTGACTTAAGATATGATTATAAAATAGTTGACATCAATGACTTTTGCCTTAATACAGACTTTAATATCATTAAATATGCTCCAAGTAAAAGAATGCTTTTTGTAGATACTTTAATTACCAAAAAAGACTTTAAAGACTTAGAAGAAATTGCTAAGAAAAATAAAGCAAATATTCTATTCTACTTTGTTTATGAAAATGGTGAAATAGTTCACACCAACTTTGCAAACAAAGTTAAAGATGCAGCTTTAGAATTAGTCAAAAATAACAAAAACAAAAATGGTTCTTACTTTATAGTTGCTAATACTTATGATCACGCATCTCAAGCTTTAGGTGCAGTTAGAGTTGAATTAAATGAAAGATTTAATTATGCAAGAAAGGAATGA", "aa": "MCSGFEKYYQFARCFRDEDSRKDRQPEFTQLDIETSFLNVEDFQKYIEKLFKHIFKSIGIKIKTPFPRVKYFDALRDYGTDKPDLRYDYKIVDINDFCLNTDFNIIKYAPSKRMLFVDTLITKKDFKDLEEIAKKNKANILFYFVYENGEIVHTNFANKVKDAALELVKNNKNKNGSYFIVANTYDHASQALGAVRVELNERFNYARKE", "aa_hexdigest": "51c2a9d7caf6eb43fe2c14f49eb0fd24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 185, "aa_cov": 0.8564593301435407, "hmm_cov": 0.7513513513513513, "evalue": 3.2175941873063524e-34, "score": 114.67032623291016, "start": 1, "stop": 179 } ], "seq_stats": { "molecular_weight": 24829.998800000016, "isoelectric_point": 8.735740089416506 }, "id": "EFPKPENHBP_1353", "locus": "GCA000186005_01340" }, { "type": "cds", "sequence": "contig_1", "start": 735506, "stop": 735949, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25", "PFAM:PF01336.31" ], "nt": "ATGACTTTTATTGACTTAAGAGACCGCTATGGAATTACCCAATGTGTCTTTAAAACAGACTTAAACGTCACAAAAGAAAGTTCAATGGAAGTAATAGGGAAGGTTGTTTTAAGAAAACAAAAGAACCCTAATCTAGCTACTGGTGATATTGAAGTAGTAGTTGATAAATACACAATTTTTTCTGAAGCAAATGAAGAATTACCTTTTGCAATTAGAGATGATATTGATGTTAAAGAAGAAACCAGAATGAAATATCGTTTCTTAGATTTGCGTCGTCCAGTAATGCAACAAAACATTATCACTAAAGATAAAATTATGTTTGCTGTTAGAGAATTCATGCACCAAAATGATTTTATTGATATTGAAACTCCAATGCTTGCTAAAAGTACACCTGAAGGTGCTAGAGATTTTTTAGTACCTACTAGAAATAGAGATGAATTTTGA", "aa": "MTFIDLRDRYGITQCVFKTDLNVTKESSMEVIGKVVLRKQKNPNLATGDIEVVVDKYTIFSEANEELPFAIRDDIDVKEETRMKYRFLDLRRPVMQQNIITKDKIMFAVREFMHQNDFIDIETPMLAKSTPEGARDFLVPTRNRDEF", "aa_hexdigest": "f154b9af50561ab94a591ddd296e2f1d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 68, "aa_cov": 0.4557823129251701, "hmm_cov": 1.0, "evalue": 5.590808579545003e-23, "score": 77.75453186035156, "start": 78, "stop": 144 }, { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 65, "aa_cov": 0.4013605442176871, "hmm_cov": 0.8923076923076924, "evalue": 1.402746034197251e-07, "score": 27.71599006652832, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 17285.704099999988, "isoelectric_point": 5.311058235168456 }, "id": "EFPKPENHBP_1354", "locus": "GCA000186005_01341" }, { "type": "cds", "sequence": "contig_1", "start": 736055, "stop": 737047, "strand": "-", "frame": 3, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_A0A0F5H223", "UniRef:UniRef90_C4XG44" ], "nt": "ATGTTTCGTTACGAACAACCACAAAAAGGACGTTATAGACAATTTAATCAAGCAGGGATGGAAATAGTTGATACAGTTATTAATCCATATAATGATGCTGAATTAATTATTGCAGCAGCTTCACTATTACAATGTCTTGATGTTAAATGTGTTTTAAAAATTAATTCAATTGGTGATGAAGAAACCAGAACTAGATATCAAGAAGAATTAAAAAAATATTTTGCTAAATATAAAGATCAATTAAATCCAATTAGTCTTGAAAGACTTGAAAATAATGTTTTAAGAATTCTTGATGATAAAGAAGAAAGGCAAAAAGATTTTGTTAAAAAAGCTCCTAAAATTAATAAATACTTAAGCAAAAACAGCACTGAATTTTTTAATAAATTAACTTCAATTTTAGACTTCAACAACATCAAATATCAAGTTGATTATTCTCTAGTTAGAGGACTTGATTATTATGATGAAGTTGTTTATGAATTTGTTTCAACTTCAAAAGAATCAGGAAGTCAAAGTACAATAATTGGCGGTGGAAGATATTCGAACTTAATCAAAGAATTAGGTGGACCTCAATTATATGCTTGCGGTTTTGGTTTTGGAGTAGATCGAGCAGCTGACATTATTAATGATGAAAACAAAGACAATAATGATGCTGAAAAAATAAACATTTTAGTAGCCACAACCAGTGAAAAAAATCTAGACATTTTATTTTCATTAACTAATGAGCTAAGAAATTATGGAAGCAAAATTGAATTTATTAAAGAAGTAGTTAAAAGCAAAAAAATCTTTGATAAGGCTCAAAAATTACAAGCTGATGTTGTGATTTTTGATGACAACATTCAAGGTCGTGAAACCTTTGTTGCTAAAAGTTTAAGCGATAATGATCGTATTATGTTCCAATACAATGAAGATGGTTATGCAGACTTATTAGACTTTTTAGCAGAACATAATTTACTAAGCGATTTAATAGCTGAAGAAGATGAAGACGAGGAATAA", "aa": "MFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEERQKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE", "aa_hexdigest": "164d7510889c1fd474910838282a6592", "start_type": "ATG", "rbs_motif": null, "genes": [ "hisS" ], "truncated": "3-prime", "pseudogene": { "start": 736055, "stop": 737347, "observations": { "mutation": [ 737092 ], "stop": [ 737092 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG44", "query_cov": 1.0, "subject_cov": 0.7268722466960352, "identity": 1.0, "score": 630.0, "evalue": 7.54e-226, "gene_start": 1, "gene_end": 330, "reference_start": 125, "reference_end": 454, "reference_sequence": "MYIIFNIKLSIYFRRHKMFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENKWYATLKNTKFAYYGPMFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEERQKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE" }, "paralog": false, "identity": 0.9976744186046511, "score": 847.0, "evalue": 6.17e-314, "description": "Internal stop codon at 737092. Nonsense mutation around 737092.", "psc": { "uniref90_id": "UniRef90_C4XG44", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A0F5H223", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG44", "EC:6.1.1.21", "UniRef:UniRef50_A0A0F5H223", "KEGG:K01892", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H223" ], "product": "Histidine--tRNA ligase" } }, "id": "EFPKPENHBP_1355", "locus": "GCA000186005_01342" }, { "type": "cds", "sequence": "contig_1", "start": 737090, "stop": 737368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "ATGTTTAACCGAATTAAAGGAACAAGAGACTTTGGTCCACAAGAAAATAATGTGATTGAATTAATTAGAAGTGCATTTGTTAATTATGCAAGAAATTATAATTTCCATTTAATTGAAACTCCAATTATTGAATCAGCCACTCTTTATCGTCGTTCAGTTGCAAGTAGCGATATAGTTAAAAAAGAAATGTACGAATTTAAAGACAAAGGTGATCGTGATATTGCACTAAGACCAGAAGGAACAGCAGGTTTTGTTAGAGCTTTAGTTGAAAACAAATGA", "aa": "MFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENK", "aa_hexdigest": "9a6234ea33c88e0ae5f1c82fd56bb223", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 84, "aa_cov": 0.8913043478260869, "hmm_cov": 0.9404761904761905, "evalue": 6.971609248160096e-10, "score": 34.91695785522461, "start": 7, "stop": 88 } ], "seq_stats": { "molecular_weight": 10642.96799999999, "isoelectric_point": 9.045768547058106 }, "id": "EFPKPENHBP_1356", "locus": "GCA000186005_01343" }, { "type": "cds", "sequence": "contig_1", "start": 737534, "stop": 737707, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTACTGCTATGCTAATAGTAATTAGTGGCAGCAATGGTGCTGGTATTTATTTTCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATCTTTTGTTGA", "aa": "MKKAKDNLNQKVNKRKKISFFTAMLIVISGSNGAGIYFRSESILNNSHQSLILAIFC", "aa_hexdigest": "4d61d16f94456fda6743f89e04ab57f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6389.474499999997, "isoelectric_point": 10.227280616760254 }, "id": "EFPKPENHBP_1357", "locus": "GCA000186005_01344" }, { "type": "cds", "sequence": "contig_1", "start": 737831, "stop": 737971, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAAACTTTATGACTTACTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTACAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCGTTTGTGATTGGCACTAAATATGATTGA", "aa": "MSKNFMTYLYLPLSYFLMPLYALMSLQQGVGAILNQEAFVIGTKYD", "aa_hexdigest": "13da91fbf543d50881c93dd170be8092", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5285.2212, "isoelectric_point": 5.946972846984862 }, "id": "EFPKPENHBP_1358", "locus": "GCA000186005_01345" }, { "type": "cds", "sequence": "contig_1", "start": 737996, "stop": 738448, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTAATTTATTTTTCAATAGTACCAGCTCTTTATACTAAAGCAGGTGATATTCAAAATAAAATTATGTTAGCTGTTAAATTTTTGCCTTTATTCTTTATTATCATTATTGGTTTTGTTTTGGCTTTTAGTTCAAAAGGTGAAGTAAATCAAACTCATTGGAATTTACAAGATCAACACTTTGATGTTAGATTGGGAAGTTCAATTGCTTCTTATAAATCTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATCTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCCATTTCAATGAGTATTAATGGTGGTTCATTTATTAAAATGAAGGATTATTTAAGTAACTGA", "aa": "MIVIYFSIVPALYTKAGDIQNKIMLAVKFLPLFFIIIIGFVLAFSSKGEVNQTHWNLQDQHFDVRLGSSIASYKSLGAGFGVFLSVSAIFFVYDGFYISAGIQSEMKEPQKTPLALLFGLLITTIIYLLIAISMSINGGSFIKMKDYLSN", "aa_hexdigest": "494bc9d6f6491d71f00d46eb57526dd8", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16623.47759999999, "isoelectric_point": 8.831669044494632 }, "id": "EFPKPENHBP_1359", "locus": "GCA000186005_01346" }, { "type": "cds", "sequence": "contig_1", "start": 739326, "stop": 739697, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTTAATTCCCGGAGGTGGAGAGGCAAATATAAAATATCATAAAGGTAAATTTGTTACGGGAGATAATAGAATTGGACAAGTTCTAAATAAAAATGAAGTTGCAACAAAATTTTTATATTATTACTTTCTTTTAAATCTTGATTTAATTAGAAAAAATTTTAGAGGTGGTAGTATAAAGCATCCGTTTATGAAAAATATTTTGGAATTAAATATACCTATTCCTCCTTTAGAAACCCAAAACAAGATAGTTTCAATTCTTGACAAATTATCAGAATACTCTCAAGAAATTAATTTAGGTTTACCTGCCGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAA", "aa": "MLIPGGGEANIKYHKGKFVTGDNRIGQVLNKNEVATKFLYYYFLLNLDLIRKNFRGGSIKHPFMKNILELNIPIPPLETQNKIVSILDKLSEYSQEINLGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "837053176ece321e545d9cb7b07dfca1", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.9896907216494846, "evalue": 2.880824303164448e-18, "score": 62.966827392578125, "start": 10, "stop": 106 } ], "seq_stats": { "molecular_weight": 14290.483499999986, "isoelectric_point": 9.396927833557129 }, "id": "EFPKPENHBP_1360", "locus": "GCA000186005_01347" }, { "type": "cds", "sequence": "contig_1", "start": 740175, "stop": 740285, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTTGGAAAACCAGTGATTGCTGATATAAAAGGTTGTATTCATGATGGCTGGTTATTAATAGCAAATTTTGAAAAAAATGTAACTTCAAAATTTTTATATTATTGA", "aa": "MSFGKPVIADIKGCIHDGWLLIANFEKNVTSKFLYY", "aa_hexdigest": "be1cc584f713b8b7d4e325832740a79c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4119.803000000001, "isoelectric_point": 7.90674114227295 }, "id": "EFPKPENHBP_1361", "locus": "GCA000186005_01348" }, { "type": "cds", "sequence": "contig_1", "start": 740409, "stop": 740549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTACAAAAGTTATTAGATAAGTATTGTCCAGATGGATATGAGTGGGTTACATTGGGCGAAATATCAAGCATTAGGAGAGGTGCCTCTCCTAGACCTATATCTTCTTTTTTGTCTAAAGAAGGTTATCCATGA", "aa": "MSYLQKLLDKYCPDGYEWVTLGEISSIRRGASPRPISSFLSKEGYP", "aa_hexdigest": "7ae243b45b3552fb620c88577b9b2e05", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5239.9313999999995, "isoelectric_point": 7.869220542907715 }, "id": "EFPKPENHBP_1362", "locus": "GCA000186005_01349" }, { "type": "cds", "sequence": "contig_1", "start": 740583, "stop": 741083, "strand": "-", "frame": 2, "gene": null, "product": "HTH domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "nt": "ATGATTTCTATATTTAAAGATAGAATTGAAATTGATTCTATGGGAAATTTAGGACCTGAACAAACAATAGAAGGTTTTTACAAAGGAGAATCAAAACCAGTTAATGAAAAGTTAGCCGAAATTTTTCTTCAATTAAGAATTAGTGAAAAATCAGGACGTGGAGTACCTGCAATAGTAAAAGTTTATGGCAAAGAAGCTATTTATTTTGGTGATGGATTTATTAAAGTTACAATTCCTTTTAATAGAATTAATGCTGTAAAATTTGAAATTGAAGTTGAAGAGATAGATGAAGAAACAGCTAAAAAAATTCTTAATCAAATAAGAAAAAAGATATTATCGGAAATTATTAAAAATCCACATATTACTAAAGCTTTATTAGCAGAAAAATTAAATCTAAGCACAACAGCAATAGACAACAATATTAATTTTTTAAAAAAACATGGTTTTATTAAAAGAGTTGGAGCAAATAAAAATGGTTATTGGAAGGCATTAATAAAATAA", "aa": "MISIFKDRIEIDSMGNLGPEQTIEGFYKGESKPVNEKLAEIFLQLRISEKSGRGVPAIVKVYGKEAIYFGDGFIKVTIPFNRINAVKFEIEVEEIDEETAKKILNQIRKKILSEIIKNPHITKALLAEKLNLSTTAIDNNINFLKKHGFIKRVGANKNGYWKALIK", "aa_hexdigest": "a49de4b4edd527e5d86e6c1466d6061b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X8GT79", "query_cov": 0.9879518072289156, "subject_cov": 1.0123456790123457, "identity": 0.573, "score": 176.0, "evalue": 7.64e-54, "valid": false, "product": "Winged helix-turn-helix DNA-binding protein", "uniref50_id": "UniRef50_D3MT65" }, "pscc": { "uniref50_id": "UniRef50_D3MT65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "product": "HTH domain protein" }, "genes": [], "id": "EFPKPENHBP_1363", "locus": "GCA000186005_01350" }, { "type": "cds", "sequence": "contig_1", "start": 741102, "stop": 741359, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATAATTCTGGATTTCCAGTTAGAGTTTCTATTTTTGGAGGTTATGACAAAACAACCCCTTTATATACAGTAGATGAATTTGGATTTACTAATTTACTTTATACACTAGATGATTTGATTAGATTTGGAAATGTAGTAAATATTTATCAAGCTGATGAAAGAAACAGAGTTCTCACAAGAAAAGAAGTACCTTTATTTGATTCTAATGTTTACAAGGAAGCAATAATTAATGCCATCTTACATAACAAATGA", "aa": "MSDNSGFPVRVSIFGGYDKTTPLYTVDEFGFTNLLYTLDDLIRFGNVVNIYQADERNRVLTRKEVPLFDSNVYKEAIINAILHNK", "aa_hexdigest": "cda976f63e031c812f605beb8c012c8d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9763.917699999993, "isoelectric_point": 5.173166847229003 }, "id": "EFPKPENHBP_1364", "locus": "GCA000186005_01351" }, { "type": "cds", "sequence": "contig_1", "start": 742131, "stop": 742622, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "nt": "ATGTCAGTTGTATTACAAGATTCATTTATGTTTAATGAAACCATTATGGACAACTTAAAAGTAGCTTCAGACAAAATCACAAACCAAGAAGTATATGAAATGGCTAGTTTAACTTCAGCTCATAACTTTATTCAAAAACTTGAAAAAGGTTATGACACTGTAATAGAAAATAGTGGTCAAAGTTTAAGTCAAGGTGAACGTCAATTGTTATCAATTACTCGGGCACTGTTGGGTAAAAAGAAAGTATTGATCTTAGATGAAGCCACAAGTAATGTTGATTCAAATACTGAACAAATTATTCAAAAAGCTTTACAAGAAGAATTAATGAAAGATCGTACTTCAATAGTTATAGCTCACCGTTTAAGTACTATTAAAAATGCGGACTTAATTTTAGTGGTTGATGATGGTCAAATTATTGAACAAGGAAATCATGCATCATTAATGGAAGCAAAAGGTTATTACTTTAATTTATATGAAAATCAATTCAAATAA", "aa": "MSVVLQDSFMFNETIMDNLKVASDKITNQEVYEMASLTSAHNFIQKLEKGYDTVIENSGQSLSQGERQLLSITRALLGKKKVLILDEATSNVDSNTEQIIQKALQEELMKDRTSIVIAHRLSTIKNADLILVVDDGQIIEQGNHASLMEAKGYYFNLYENQFK", "aa_hexdigest": "523b4f3bf27f526a669a4635f9842b70", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00261D2CD4", "query_cov": 0.9938650306748467, "subject_cov": 0.9700598802395209, "identity": 0.562, "score": 174.0, "evalue": 6.51e-53, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A257LJQ8" }, "pscc": { "uniref50_id": "UniRef50_A0A257LJQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1365", "locus": "GCA000186005_01352" }, { "type": "cds", "sequence": "contig_1", "start": 743199, "stop": 743438, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTGTCGGTTTTACTTTAACAGCAATAGTAATTCCTTTATCAGTTTTATTCTTCTCACTTGGCTACTTCATGATTTCAAAAGCTAGAAAATCATATGTTCTTGTACAACAAGACTTTGGTGATTTAAATGCTTATGTTGAAGAAATGCTAACTAATAGCAAAATCACTCAAACTTTTGATCGTCAAGAAACAGCAGAAAAGAATTTCAAAAAAATAACTCATGATATTTATTGA", "aa": "MAFVGFTLTAIVIPLSVLFFSLGYFMISKARKSYVLVQQDFGDLNAYVEEMLTNSKITQTFDRQETAEKNFKKITHDIY", "aa_hexdigest": "fdfdb893b78e48f911fdba37f6ccc169", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9124.468399999996, "isoelectric_point": 6.541281318664551 }, "id": "EFPKPENHBP_1366", "locus": "GCA000186005_01353" }, { "type": "cds", "sequence": "contig_1", "start": 743475, "stop": 743603, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTTATTGAAATGCCAGTTAGCTTTTATGATAAAGAAAAAAGTGGTGACTTAATTTCAGTATTAATCAATGATATAAATAACTTAGCAAACTCATTTGTTCAAATGTTCAATGAAACTTGA", "aa": "MEKLIEMPVSFYDKEKSGDLISVLINDINNLANSFVQMFNET", "aa_hexdigest": "6178115e24ce4af06281e576f40eaf8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4838.4865, "isoelectric_point": 4.196388816833497 }, "id": "EFPKPENHBP_1367", "locus": "GCA000186005_01354" }, { "type": "cds", "sequence": "contig_1", "start": 743986, "stop": 744384, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGCGTGAAGCACTTAAAAATGCTTGTGCTGATGATTTTGTTAATAAATTTGAAGATGGTTTAGATCACAAAGTTGTTCAAGGAGCTAGAAACCTTAGCGGTGGTCAAAAACAAAGAATTTCAATAGCTCGTAGTTTATTAAGAAAACCTAAAATCTTAATTTTAGATGATTCAACAAGTGCACTAGACAATATTACAACTAGAAAAGTTATTAATAATATTAAAAATAATTATGACTGTTCAACAATATTAATTAGCCAAAAAATTGGAGCTATTAAAAATGCTGACAATATTATGGTTTTAACTAATGGAGCAGTAATGGATCAAGGTAAACACGGTCACTTAATTAAGACCTGCGATTTTTACCAAAAGATCTATGAAACACAGCTAGAACAATAG", "aa": "MREALKNACADDFVNKFEDGLDHKVVQGARNLSGGQKQRISIARSLLRKPKILILDDSTSALDNITTRKVINNIKNNYDCSTILISQKIGAIKNADNIMVLTNGAVMDQGKHGHLIKTCDFYQKIYETQLEQ", "aa_hexdigest": "49ff5100b3412023d25897d9a9573dda", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.614, "score": 167.0, "evalue": 1.01e-50, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_1368", "locus": "GCA000186005_01355" }, { "type": "cds", "sequence": "contig_1", "start": 744412, "stop": 745008, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATTTTTGCTTTTGCTGTACCGATTTTTTATGCTTCAATTAACTTATTAATTATTGGAATCTATGCTTTTGCAGGTCATAGAATGTATACTGAAGTTGCAACTTTAGATTCACTTGTTAAAGTTAATATTTTTATAGAATACTTATTTTCAATTAGCTTTGGTCTCTTAATGACCATTCAATTCTTAACATCAATGTTTAGAGCTCGTGTTTCAGCAGGTAGAATTAATGAAATTTTAGAAACCAAAATAGATCCTTTATTTATTAAAGATGGTAAACAATTAACAAATAATTTTGATCTAGAAGTTCAAAATCTTTCATTTCGTTACTATGAAACCAGCCCTTCATATTCACTAATGAATATAAATGTTAAATTACCTTATAAACAAACTTTAGGTATTATTGGACCAACAGGTAGTGGTAAAAGTACATTTATTAACTTACTTTTAAATAATTATGTTTATGATGAAGGTTCAATTAAAATTGGAGGCCAAGAAGTTAAAGAAATTAATACCAAAAACCTTCATGAAACTGTTGGCATAGTTTACCAAGAAGCTTTACTTTACACTGGAACAATTAGATCAAACTTACTTTGA", "aa": "MIFAFAVPIFYASINLLIIGIYAFAGHRMYTEVATLDSLVKVNIFIEYLFSISFGLLMTIQFLTSMFRARVSAGRINEILETKIDPLFIKDGKQLTNNFDLEVQNLSFRYYETSPSYSLMNINVKLPYKQTLGIIGPTGSGKSTFINLLLNNYVYDEGSIKIGGQEVKEINTKNLHETVGIVYQEALLYTGTIRSNLL", "aa_hexdigest": "b7d2e9fbc18ce98e3055e89f9cb0be74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 77, "aa_cov": 0.3838383838383838, "hmm_cov": 1.0, "evalue": 1.0207440518334262e-16, "score": 58.117149353027344, "start": 119, "stop": 194 } ], "seq_stats": { "molecular_weight": 22367.73180000001, "isoelectric_point": 6.737148475646973 }, "id": "EFPKPENHBP_1369", "locus": "GCA000186005_01356" }, { "type": "cds", "sequence": "contig_1", "start": 745033, "stop": 745245, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTTAGTGTTTTAGCTGTTGTACCTGTAATTTTTGTACTAGTACTTGTTATTGGTTTAACTACTAATCCACTTTTAAAGAAAACACAAAAAGTTGTTGAAGAAATTACTAAAGCAACTGATGAAAATATTTTAGGAGCAAGAGTTATTAAAACTTTTAACTTAGAAGAAACAAGAAAAAAACGTTTTAGTGAATACAACCACAGATGA", "aa": "MSLSVLAVVPVIFVLVLVIGLTTNPLLKKTQKVVEEITKATDENILGARVIKTFNLEETRKKRFSEYNHR", "aa_hexdigest": "5035845b45203479abbec87401ce82ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.3474, "isoelectric_point": 9.773100852966305 }, "id": "EFPKPENHBP_1370", "locus": "GCA000186005_01357" }, { "type": "cds", "sequence": "contig_1", "start": 745582, "stop": 745767, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAGTTAATTAAAATGCTACCAATTAAGATTAAATTAGCATTTCTTTTTGGTTCATTGCTTGTTTTTATTAATATTATTTTTACTTTGCTTTTGCCTAATATTATTTCTCAGTTTATTAGATTATTATTCGTTGATGATTATTCAAAAGTTGAGACATTATCCTTCTTTAATGGTCGTTGA", "aa": "MFKLIKMLPIKIKLAFLFGSLLVFINIIFTLLLPNIISQFIRLLFVDDYSKVETLSFFNGR", "aa_hexdigest": "316671a72d6d69cb3154fa8398c32412", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7154.733899999997, "isoelectric_point": 9.994614219665529 }, "id": "EFPKPENHBP_1371", "locus": "GCA000186005_01358" }, { "type": "cds", "sequence": "contig_1", "start": 746110, "stop": 746343, "strand": "+", "frame": 1, "gene": null, "product": "Nudix hydrolase domain-containing protein", "db_xrefs": [ "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_C4XG50", "UniRef:UniRef90_C4XG50" ], "nt": "ATGAAAGAAAAATATGTTTTACCTAATGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATGCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGATGAAAAAAAGAATAAATAA", "aa": "MKEKYVLPNGIPKEVVYFLAMQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLDEKKNK", "aa_hexdigest": "bd8e15c55a65609500915f50e1fb41a0", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 745882, "stop": 746343, "observations": { "mutation": [ 745897, 745960, 745996 ], "stop": [ 745897, 745960, 745996 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG50", "query_cov": 1.0, "subject_cov": 0.5032679738562091, "identity": 1.0, "score": 149.0, "evalue": 1.5e-44, "gene_start": 1, "gene_end": 77, "reference_start": 77, "reference_end": 153, "reference_sequence": "MFQKKWGLNMSEKSCGLVIFRQFTKGWKVLIAKQINNMWSFVKGHVENKESEIETALREAKEEVNLENFQIFKETRMKEKYVLPNGIPKEVVYFLAMQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLDEKKNK" }, "paralog": false, "identity": 0.9738562091503268, "score": 293.0, "evalue": 9.69e-104, "description": "Internal stop codon at 745897, 745960, 745996. Nonsense mutation around 745897, 745960, 745996.", "psc": { "uniref90_id": "UniRef90_C4XG50", "product": "Nudix hydrolase domain-containing protein", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_C4XG50", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG50", "EC:3.6.1.17", "UniRef:UniRef50_C4XG50", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG50" ], "product": "Nudix hydrolase domain-containing protein" } }, "id": "EFPKPENHBP_1372", "locus": "GCA000186005_01359" }, { "type": "cds", "sequence": "contig_1", "start": 746423, "stop": 746926, "strand": "-", "frame": 3, "gene": "pgmB", "product": "beta-phosphoglucomutase", "db_xrefs": [ "EC:5.4.2.6", "GO:0000287", "GO:0005975", "GO:0008801", "SO:0001217", "UniRef:UniRef50_A0A5B8JY41", "UniRef:UniRef90_A0A3P8K8F6" ], "nt": "TTGCCTCGTAGAGATACTTTACTTGCAATATTTGAATTAAAGAAATTTAAGCATAATTTTGATGATCAAAAAATCAATGAATTATGTACTAAAAAGAATGAATTATATTTAAGTTATTTAAAAAAATATATTAATAAAGATAGCCTTTTAGAAGGCATCGAACAATTTTTAAAAGACTTAAAAAAAGATAAGATTAAAATTGCAATAGCTTCTTCAAGTCTCAATGCTCCTTTGATTTTAGAAAAACTCGGAGTTATAAATTATTTTGATGTTATTGTTAATCCTGTTGAAGTTAAAAAAGGAAAACCTGCACCAGATATTTTTCTTTTAGCTCAAGAACAACTTAAATTAAAAAAAGAAGAATGTGTTGGAATTGAAGATGCAGTTGTTGGAGTTGAAGCTTTAAATAAGGCTAACATTAAATCCATTGCAATAACAAACGGTAATAATAATTTATTCCAAAAAGCAACTTTATTAGTTCAAGACACTTCTAAATTAATTTGA", "aa": "MPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLI", "aa_hexdigest": "62ea9c14ed358c8169efc4738cc1a76d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pgmB" ], "truncated": "both", "pseudogene": { "start": 746396, "stop": 747118, "observations": { "mutation": [ 746425, 746992, 747067 ], "stop": [ 746425, 746992, 747067 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F6", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.9940000000000001, "score": 318.0, "evalue": 1.55e-108, "gene_start": 1, "gene_end": 167, "reference_start": 65, "reference_end": 231, "reference_sequence": "MLMVWTVKLQMKSKFSRWKMIKGIIFDLDGVITDTAKLHYKAWKEIVNELGIDYSKEENEKLRGLPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLIWDLIKPILF" }, "paralog": false, "identity": 0.9833333333333333, "score": 454.0, "evalue": 7.79e-165, "description": "Internal stop codon at 746425, 746992, 747067. Nonsense mutation around 746425, 746992, 747067.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F6", "gene": "pgmB", "product": "beta-phosphoglucomutase", "ec_ids": [ "5.4.2.6" ], "uniref50_id": "UniRef50_A0A5B8JY41", "go_ids": [ "GO:0000287", "GO:0005975", "GO:0008801" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F6", "EC:5.4.2.6", "UniRef:UniRef50_A0A5B8JY41", "GO:0000287", "GO:0005975", "GO:0008801" ] }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JY41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JY41" ], "product": "Beta-phosphoglucomutase" } }, "id": "EFPKPENHBP_1373", "locus": "GCA000186005_01360" }, { "type": "cds", "sequence": "contig_1", "start": 747360, "stop": 748025, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03632.21" ], "nt": "ATGAAAAAAATAGGTTATGAAATGATAATTGATACAGCTTACTTTTACACAAACCGTGCAGAAATACAACCAGATGGTAGTTATGAAATTAAAGATGTAATGGGTCCAAATGAATATAAAGGAAACATTAATAATAATGCATTTATCAACCAAATGGCCTTATTTAATATGAAATTGGCAATTTCAATATATCGCAAATTGCAAGCACAAACACCAGGCTTACTTAACTGTATTTTAAAAAGAATACCTTATAAAATTAATTTTTCAAAAATGACTAAAGTCATTAAAGGTCTTAAAATTCAAGCTCCTAATAAAGATAAAATTATTGCAGAAAATGATACATTCTTATCCTTACCTTTAAAAAATGTTAGACCATTTCAAATGCTTGGAGATGCTGGTAAAAAATTATTCAATACTTCTGAAGGACATAGATTATTAGGCTCTCAATTAGTAAAACAAGCCGATGTCATTCTCTTAACTTATTTATTCCCTGAGTTATATAATAAAGAAATTAGAGAAAAAAACTTTGATTACTATGAAGCAATAACCACACATGATTCATCACTTTCGGCAGCAACTTATTGTATTGAAGCTATTAGATTAAATAAAATTGAAAAAGCTTATGAATTATTTAAATATGGTATTAATATTGACTTTGGTCCATGA", "aa": "MKKIGYEMIIDTAYFYTNRAEIQPDGSYEIKDVMGPNEYKGNINNNAFINQMALFNMKLAISIYRKLQAQTPGLLNCILKRIPYKINFSKMTKVIKGLKIQAPNKDKIIAENDTFLSLPLKNVRPFQMLGDAGKKLFNTSEGHRLLGSQLVKQADVILLTYLFPELYNKEIREKNFDYYEAITTHDSSLSAATYCIEAIRLNKIEKAYELFKYGINIDFGP", "aa_hexdigest": "30b817bdc780bf80d9fe33155f5fcfe2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03632.21", "name": "Glyco_hydro_65m", "length": 221, "aa_cov": 0.9909502262443439, "hmm_cov": 0.9638009049773756, "evalue": 1.1933443440068907e-55, "score": 185.2943115234375, "start": 1, "stop": 219 } ], "seq_stats": { "molecular_weight": 25390.247700000007, "isoelectric_point": 9.173480415344237 }, "id": "EFPKPENHBP_1374", "locus": "GCA000186005_01361" }, { "type": "cds", "sequence": "contig_1", "start": 748527, "stop": 749423, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATATTGAATATGACCCTGATAATAATCAGATTTCACAGATTAAATTTGACCGTAATATAACAGCAAAAAGTGAAAGTATTTTTTCACTAGGTAATGGTTATTTAGGTATTAGAAGTGTTGATGAAGAAGTTCAAGAATATAACAAAGAAGACTTTTTTGTTAATGGTATTTTTAATAGAACATCTAAAGGAGAAGTTCCTGAATTAGCTAATTTAGCTGATTTAATTCAAACTCCTATTTCTTTTAATAATCAAATTTTTGAAGTTAATGAAAAGGATCAATATACTAAAACTTTAGATATTAAAAATGGAATTTTAAGTCGTAAAGTCATTGCCAAAAGAGACTTTGCTACAATTGAATTAAATTTTGAACGTTTTGTTTCTCAAGAAATAAGAAACATTTATGCTCAAAAAATTACAATTAAAATTTTAGATTCTAAAGATGAAATTGTTAAGGTTAAATTATTACCAGGAATTAATGGACAAGTAACAAACAGTGGTACTCAACACTTTGAAGAAGGTCTTAAAAACCGTGTGACAACCGAAAGTTTGAAGATGCAACAAATAACAACTAATTCAAATCGTTTTGTTGTGCACCACATGTTTACAAGACTTTTAAAAAATAATAAGTTAATTTTAGGCAGTAATGACAAATATCAAATTCAAACTGATCGCCGCAAAGTTTACTTTAAAATCGAAAATGAATTAAAAGCAAACGAAGAATTAGTTTTAGAAAAATTAATGTCAGTTCATACATCAATTGATGATGAAGAAAAAATATTAACTAATAGTGAAGTTTTGGCTAAATCAGATTTAACTTTTAACTACTTATTAAAAAATACATATGCTTCATTAAAAGAAAATTCTACAGCTGCAATGCAAAAAGTTTGA", "aa": "MEYIEYDPDNNQISQIKFDRNITAKSESIFSLGNGYLGIRSVDEEVQEYNKEDFFVNGIFNRTSKGEVPELANLADLIQTPISFNNQIFEVNEKDQYTKTLDIKNGILSRKVIAKRDFATIELNFERFVSQEIRNIYAQKITIKILDSKDEIVKVKLLPGINGQVTNSGTQHFEEGLKNRVTTESLKMQQITTNSNRFVVHHMFTRLLKNNKLILGSNDKYQIQTDRRKVYFKIENELKANEELVLEKLMSVHTSIDDEEKILTNSEVLAKSDLTFNYLLKNTYASLKENSTAAMQKV", "aa_hexdigest": "a1b45e26078bfdf17cfe32092316992b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34425.60590000004, "isoelectric_point": 5.952997779846191 }, "id": "EFPKPENHBP_1375", "locus": "GCA000186005_01362" }, { "type": "cds", "sequence": "contig_1", "start": 749433, "stop": 749558, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACATCAAAAAGTAGTTGAAAATGTTCAAGGAAAACAAATATTATCAACATATATTGATAACAAAAAAGTTACTGATAAATTAAGACCTTTTGAGTCGATTATGTTAGAAATTAAATAA", "aa": "MTKHQKVVENVQGKQILSTYIDNKKVTDKLRPFESIMLEIK", "aa_hexdigest": "f0dee4995cbcbd89a904d5f347d56a20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.654999999999, "isoelectric_point": 9.52289905548096 }, "id": "EFPKPENHBP_1376", "locus": "GCA000186005_01363" }, { "type": "cds", "sequence": "contig_1", "start": 749823, "stop": 750038, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00128.31" ], "nt": "ATGCTTTTTGGGCTAAAAGGTATTCCTTGTATTTATTATGGAGAAGAAATTGGTTTATTAAATAATCAATTTAACTCAAGAGAAGAATTTAAAGATTGTGACATATTTAATGCCTTTAGTGAGTTAGTAGATAAAAAGAAAATTTACAGTGAATACGAATTTATTAAATATTGCAATATCAACAGTCGTGACTCAGGCCGCACTTTGATGCAATGA", "aa": "MLFGLKGIPCIYYGEEIGLLNNQFNSREEFKDCDIFNAFSELVDKKKIYSEYEFIKYCNINSRDSGRTLMQ", "aa_hexdigest": "b957924e0ae6890f9dff0f674016da08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00128.31", "name": "Alpha-amylase", "length": 70, "aa_cov": 0.9859154929577465, "hmm_cov": 0.7285714285714285, "evalue": 2.0877179880946309e-07, "score": 26.826805114746094, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 8391.477099999996, "isoelectric_point": 4.990997886657714 }, "id": "EFPKPENHBP_1377", "locus": "GCA000186005_01364" }, { "type": "cds", "sequence": "contig_1", "start": 750258, "stop": 750404, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCTTAAGTTTAATAAGTTAGCTTTTAGTGATAAAAAAGATGCTTATACATTAGGTGAAGCTAGTGGAATTAATTTAGAAGAATTATTAAAATATGGAACAGGATCTAAAAAAGTAGCTGATAACTATTACAACTTTTCTTGA", "aa": "MLLKFNKLAFSDKKDAYTLGEASGINLEELLKYGTGSKKVADNYYNFS", "aa_hexdigest": "b6d4506498b4c2104deb739d609a6e3c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.071800000002, "isoelectric_point": 7.961668205261231 }, "id": "EFPKPENHBP_1378", "locus": "GCA000186005_01365" }, { "type": "cds", "sequence": "contig_1", "start": 750930, "stop": 751076, "strand": "-", "frame": 2, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "nt": "ATGAAAACAATAAAATTAGAAGATAAAATTATTTACCAAATATTTCCTCGTTCATTTTATGACTCAAATAATGATGGCGATGGAGACTTATTAGGTATAGCAAAAAAACTAGATTATTTACAAGACTTAGGTATAAATGCGATATGA", "aa": "MKTIKLEDKIIYQIFPRSFYDSNNDGDGDLLGIAKKLDYLQDLGINAI", "aa_hexdigest": "53bacd49ab9424190d5d678759b2dd5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FD1789", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.7709999999999999, "score": 80.1, "evalue": 5.15e-19, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI0021FD1789" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FD1789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "EFPKPENHBP_1379", "locus": "GCA000186005_01366" }, { "type": "cds", "sequence": "contig_1", "start": 751420, "stop": 751626, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTGCAAATTACAACACAAGGACGTCAATTCTTTTTAGCAGGTACTGAATTAGCTCAAAGTAAACCTGCTGATAAAAGTGGTCAAGATTTTCATAAGTCATTTTATTCATATGCCAAAGATTATTTTGAATTAAATTCAGATGAAAATAAATACCATTTTAATTCTTATAAAACGACAGATTATACTAACGGACTAAAATGA", "aa": "MILQITTQGRQFFLAGTELAQSKPADKSGQDFHKSFYSYAKDYFELNSDENKYHFNSYKTTDYTNGLK", "aa_hexdigest": "14bc447c73774bc7e88605fb093b9d0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7929.621499999998, "isoelectric_point": 6.706910133361816 }, "id": "EFPKPENHBP_1380", "locus": "GCA000186005_01367" }, { "type": "cds", "sequence": "contig_1", "start": 752011, "stop": 752283, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ], "nt": "ATGATGATAAATTCAATTTATGATAATATTCTGCCTGGTTACTATTATCGAGATAATGCTAAAGTAAAACCTGTTATTTATCCGCCTTTAGCAAGTCAAAGATATATGGTTAGAAAACTAATTTATGAATCACTAAAACATTTTGTTGAATATTATGATGTTGATGGTTTCCGTTTTGATTTATCAAGTTTTATTGATAAAGAAACACTTGATTATTGTTTTAAAAACTTAAGAAAAATAAAACCTAATTTAGTAATCCATGGTGAAGCTTGA", "aa": "MMINSIYDNILPGYYYRDNAKVKPVIYPPLASQRYMVRKLIYESLKHFVEYYDVDGFRFDLSSFIDKETLDYCFKNLRKIKPNLVIHGEA", "aa_hexdigest": "22ce29c00e40eccfa8031a13fb171c4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002540EC32", "query_cov": 0.9444444444444444, "subject_cov": 0.8854166666666666, "identity": 0.6940000000000001, "score": 123.0, "evalue": 7.28e-35, "valid": false, "uniref50_id": "UniRef50_UPI0021AF9781" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF9781", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10795.404299999991, "isoelectric_point": 8.62549915313721 }, "id": "EFPKPENHBP_1381", "locus": "GCA000186005_01368" }, { "type": "cds", "sequence": "contig_1", "start": 753001, "stop": 753117, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATAAAAGGACAAACTAAAGAATTTTTTAAGGACTTTGATGCTAAATATGCTTATAAAAAAGCTGATTTAGGAACAAAATTCCATAAAAATCATATTGAAATAAAATTTTGA", "aa": "MNIKGQTKEFFKDFDAKYAYKKADLGTKFHKNHIEIKF", "aa_hexdigest": "47b64872272290e3a6bff20cd568ba2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4581.255099999999, "isoelectric_point": 9.574667167663574 }, "id": "EFPKPENHBP_1382", "locus": "GCA000186005_01369" }, { "type": "cds", "sequence": "contig_1", "start": 753263, "stop": 753616, "strand": "-", "frame": 3, "gene": null, "product": "HTH gntR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07", "UniRef:UniRef50_UPI0021AECA4B", "UniRef:UniRef90_C4XG55" ], "nt": "ATGGTAGATAAAAGAATTCTCTTTGTTCAAGGTTTTAGAACTTTTGAAAAAACTTATTATAAAGATAATGAGGTTTTAGCAACTTCTGAAATTTATGTAAGCTGTAAAAATATTGAAGTTGATGAGGAAATTAATTTTAATAAATCTTTAACCGACTTATTAATTGAGCGCGATGGTTTAAATAATATGGTTTATTCATTATCTTATGAAAATGTTAAAAAATTTGGATATGAAAGCTTGGTAGTTGTTTCATTTTTTGGCTATGATGAAGATAGCATTTCTGTAGCTGGAAAGTATTATATTAATCCTAAATACTTTAGATTTTTCCATCAAGAATTTTCATTAAAATCATAA", "aa": "MVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS", "aa_hexdigest": "63039c7ad349478c37a49bd95f022305", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0021AECA4B", "query_cov": 0.9658119658119658, "subject_cov": 0.8828125, "identity": 0.513, "score": 123.0, "evalue": 5.44e-34, "valid": false, "uniref50_id": "UniRef50_UPI0021AECA4B" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AECA4B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AECA4B" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 753263, "stop": 753916, "observations": { "mutation": [ 753619 ], "stop": [ 753619 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG55", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 232.0, "evalue": 1e-75, "gene_start": 1, "gene_end": 117, "reference_start": 118, "reference_end": 234, "reference_sequence": "MFLKFKGVYNIYIMDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQWMVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 427.0, "evalue": 5.96e-155, "description": "Internal stop codon at 753619. Nonsense mutation around 753619.", "psc": { "uniref90_id": "UniRef90_C4XG55", "product": "HTH gntR-type domain-containing protein", "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG55", "UniRef:UniRef50_L0RW07" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07" ], "product": "HTH gntR-type domain-containing protein" } }, "id": "EFPKPENHBP_1383", "locus": "GCA000186005_01370" }, { "type": "cds", "sequence": "contig_1", "start": 753617, "stop": 753928, "strand": "-", "frame": 3, "gene": "gntR", "product": "GntR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "nt": "ATGGATTTACAAGAAAAGAAGAAAACACAAGTGATTATTGATTATTTAATTGATTTGATTAAAACTCAAAAAGTACCAACAAACAAAATCTTGCCAAGTGAGCATTCTTTAATGATGCGTTTTGATTGTTCAAGAAGTATTGTTGTTAAAGCATATCAAAAATTAGAAGCATTAGGAGCTATCTATTCAATTTCAAAAAGAGGACATTTTGTTGCTGAAAACTTTCACAACTTAATTAAACCAATTAGTTATCTTTTAGGAGCAACAGCTCAAGAAGGAAAAGAAATAAAAAGCAAGGAATTACCTCAATGA", "aa": "MDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQ", "aa_hexdigest": "31cec69671252884f422fec175262b5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF0E19D", "query_cov": 0.8058252427184466, "subject_cov": 0.8137254901960784, "identity": 0.711, "score": 127.0, "evalue": 2.17e-36, "valid": false, "product": "GntR family transcriptional regulator", "uniref50_id": "UniRef50_UPI0021E0F715" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0F715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "product": "GntR family transcriptional regulator" }, "genes": [ "gntR" ], "id": "EFPKPENHBP_1384", "locus": "GCA000186005_01371" }, { "type": "cds", "sequence": "contig_1", "start": 754026, "stop": 754286, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF", "UniRef:UniRef90_UPI0001E32EFF" ], "nt": "ATGATTGCTTTAATTAATAAGCTAAGAAAAGATTATTCTTTTTTAAGTTTAACTAATATAGAAACTTTAGTTGATCCAAGATTAGTAGTAGACAGTAAAGATTATTCAAATGTTATTGTCCGTAAAGATCTTAATGATGATAAGAATTATTTAATGTTTGTTATTTACAATCATAAATGTCAAAATAATGTTCTTCAAAAGATTAGCAGGAATTATCACTTTGAAGCATTATATGAATATAAATGCAAAAATATGCCATGA", "aa": "MIALINKLRKDYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMP", "aa_hexdigest": "07270961a4e46e10aa869bdcf8d83666", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 753972, "stop": 754532, "observations": { "mutation": [ 754028, 754421 ], "stop": [ 754028, 754421 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32EFF", "query_cov": 1.0, "subject_cov": 0.46236559139784944, "identity": 0.919, "score": 156.0, "evalue": 5.41e-47, "gene_start": 1, "gene_end": 86, "reference_start": 83, "reference_end": 168, "reference_sequence": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMKWKNHQEISAIYEKKLDACQSNLFLNVSNSLDYVENEIAPGNSIYNMIALINKLRKEYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMPWGIIMQKYACGIYKLTKK" }, "paralog": false, "identity": 0.956989247311828, "score": 353.0, "evalue": 3.91e-127, "description": "Internal stop codon at 754028, 754421. Nonsense mutation around 754028, 754421.", "psc": { "uniref90_id": "UniRef90_UPI0001E32EFF", "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32EFF", "UniRef:UniRef50_UPI0001E32EFF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10322.852299999997, "isoelectric_point": 8.886080360412599 }, "id": "EFPKPENHBP_1385", "locus": "GCA000186005_01372" }, { "type": "cds", "sequence": "contig_1", "start": 754419, "stop": 754532, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTATCTTTTAGCTTTGCCTGCTAAACCAATAATTTATTATGGTGATGAATTAGCATATGCAGGAACTAGAAGCTTTGATGATCCTGGTTTAAGAGAACCAATGAAGTGA", "aa": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMK", "aa_hexdigest": "389e5d2b2e414406c4f45697653c71b4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4189.8484, "isoelectric_point": 4.8610639572143555 }, "id": "EFPKPENHBP_1386", "locus": "GCA000186005_01373" }, { "type": "cds", "sequence": "contig_1", "start": 755643, "stop": 755807, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACATCAAGATTTTTATTTAAAAAATATTTTAATATTTAAAAAAGTTAAAATTATTATTATGAAAACAAACAAATTAAAAGAAAAATTTATGAACTTTATTAAAAAAATGAAACTTAAAAAATTCATTAAAAAACATCCAAACCAAAATTATGTATCTTGA", "aa": "MIHQDFYLKNILIFKKVKIIIMKTNKLKEKFMNFIKKMKLKKFIKKHPNQNYVS", "aa_hexdigest": "6e65700a5dc70567e8ee1bf50ff87fcf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6714.3002000000015, "isoelectric_point": 10.3802640914917 }, "id": "EFPKPENHBP_1387", "locus": "GCA000186005_01374" }, { "type": "cds", "sequence": "contig_1", "start": 755836, "stop": 755964, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGTTATACTAACAAACTTTATTTTGTTAATTGTTGCTAAAACAAAATATGGATATGTTAAAAATGACCAAATTATTGCTAAAAGCAAAATTGATACTAAGGACTTAGAAAAACAATTAATATAA", "aa": "MFVILTNFILLIVAKTKYGYVKNDQIIAKSKIDTKDLEKQLI", "aa_hexdigest": "9a1f1b8edc24a089397c55dd38a791a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4870.8315, "isoelectric_point": 9.45688343048096 }, "id": "EFPKPENHBP_1388", "locus": "GCA000186005_01375" }, { "type": "cds", "sequence": "contig_1", "start": 755971, "stop": 756228, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATAAATATTCTCTTCATATCTTTAATCATTAATTGTATTGCAGTAATTCGACTTTTCTCAAATAAAAGTAATGCAGACAAATACTTTATTTATCTTATATTTCTCCAATTAACCTCTTTTTGTTTATTCTTTTTTGCAATCTCACTAAGTCCTAATAAACTTAACAAAGTTATTGACTTTAAAGAATTAAGCGGTTCAGAATATGCTATATATCAATTTTTAGATAAAATGAACATTACTTATGCATGA", "aa": "MININILFISLIINCIAVIRLFSNKSNADKYFIYLIFLQLTSFCLFFFAISLSPNKLNKVIDFKELSGSEYAIYQFLDKMNITYA", "aa_hexdigest": "9cb1d343e5f21775a9d36a7a46107721", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9907.670199999991, "isoelectric_point": 8.617247200012208 }, "id": "EFPKPENHBP_1389", "locus": "GCA000186005_01376" }, { "type": "cds", "sequence": "contig_1", "start": 756340, "stop": 756498, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAGTTAACCAAAAATTAAAAAAATCTAAAACAAATTATTTAGCTCGTAAATCTTTATTAGTATTATCAATTATTATTACAATAGTTGCAATAGCAATATTGTTTGCTTTTCTCTTTTTATTCTTAAAAAGAATAGATGAAGAATTAAATTGA", "aa": "MFKVNQKLKKSKTNYLARKSLLVLSIIITIVAIAILFAFLFLFLKRIDEELN", "aa_hexdigest": "c36ec00f5fb637ce26c7203e8208a0b5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6070.406899999995, "isoelectric_point": 10.214515876770019 }, "id": "EFPKPENHBP_1390", "locus": "GCA000186005_01377" }, { "type": "cds", "sequence": "contig_1", "start": 756498, "stop": 757691, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1", "UniRef:UniRef90_C4XG58" ], "nt": "TTGAATAATACTCAAAATCAAAATGAAGATTTTGACGATTTATCCTTTGAAACAATTAATTTGGACAAAATGCTAGATGAAATCGGTGAGCTTAGAAATATTGAAAAAGGAGCTCATATTGAACTAAAAGGATTATCTAAAAAATATGAAGGTAATGAAAATTATACACTTGAAAATATTAATTTAGATATTCAACCAGGTAAATTTTGTATCTTTTTAGGACCTAGTGGTTGCGGTAAAACTACACTACTTAGAATGATTGCGGGATTAAATTCAATTACAAAAGGTGACTTATTATTCAATGGCAAAAGATATAATAACCTTTTACCTAGTGAAAGAAATATTGCAATGGTTTTTCAATCATATGCTTTATATCCTCACATGAACGTTTACAATAATATTGCTTTTGGTTTAAAAATAGCCAAAGAAAGAAAAGACGTTATTGACAAACGTGTTAAGGATGTTGCTAAAATTCTAAAAATAGAAGATTACTTATACAGAAAACCACGTGACTTATCTGGTGGTCAAAGACAACGTGTGGCAATAGGTCGTGCTATTGCTCGTAAACCTCAAGTGTTCTTGATGGATGAGCCTCTTTCTAACCTTGATGCAAAACTTCGTGAAAGTATGCGTCGTGAAATTGTTAATATCCACAGAATGCTTGGCGCAACCAGTATCTATGTAACTCATGACCAACTTGAAGCTATGACAATGGGTGATCAAATTGTTGTCTTCAATGATGGCTCAATTCAACAAAATGGTACTGGCCGTGACTTATACTTCAAACCTGCTAATGTCTTTGTAGCTAGATTTATTGGTTCTCCAACCATGAATGTTTTCAAAGCAGCATGCGAAAACGGCAAAATTGTTAGTGCTCAAAAAGACATTAAAATTAAAGCCCCAAAAGAATATTTGGAACAATTAAAAGACAAAAAATTAAAAATTGGTTTTAGAAGTGAAGACATCAAAATTCATGCTTCTGAAGTATCAAATTCAATTCATGCAACTATTAAAAATATTGAATTAATTGGTAAAGAACAACTTGTAACTGTTAAATTGGATAATGACGATATCGAACTTACAATTACAGCAAGCAATTCAGATACTTACGAGCTTTATTCAAAAGTTTACATTGAATTTTATGAACCTAGAATCCATATCTTCGATGCGGAAACAACAAAACGGATTAATTAA", "aa": "MNNTQNQNEDFDDLSFETINLDKMLDEIGELRNIEKGAHIELKGLSKKYEGNENYTLENINLDIQPGKFCIFLGPSGCGKTTLLRMIAGLNSITKGDLLFNGKRYNNLLPSERNIAMVFQSYALYPHMNVYNNIAFGLKIAKERKDVIDKRVKDVAKILKIEDYLYRKPRDLSGGQRQRVAIGRAIARKPQVFLMDEPLSNLDAKLRESMRREIVNIHRMLGATSIYVTHDQLEAMTMGDQIVVFNDGSIQQNGTGRDLYFKPANVFVARFIGSPTMNVFKAACENGKIVSAQKDIKIKAPKEYLEQLKDKKLKIGFRSEDIKIHASEVSNSIHATIKNIELIGKEQLVTVKLDNDDIELTITASNSDTYELYSKVYIEFYEPRIHIFDAETTKRIN", "aa_hexdigest": "6e4938bc3c00c1deea62cc35414349c4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XG58", "query_cov": 1.0, "subject_cov": 0.9778325123152709, "identity": 0.997, "score": 772.0, "evalue": 1.16e-281, "valid": true, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A449AYT1" }, "pscc": { "uniref50_id": "UniRef50_A0A449AYT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1" ], "product": "ABC-type maltose/maltodextrin transporter ATP-binding protein MalK" }, "genes": [], "id": "EFPKPENHBP_1391", "locus": "GCA000186005_01378" }, { "type": "cds", "sequence": "contig_1", "start": 758019, "stop": 758324, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTCAACCATTTGTGTTGCTTTGAATGCTTATGCTTATTCAAGATTTAAATTCGCTGGTTCAAAACACTCATTAACTATTATTATGATGTTGCAAATGATTCCTGCCACTTCATCTCTTATTTGTCTTTACATTATTGTGCGGATGGGGAATGATTTAGGAATTAATTCAATATTAATGCTGGTTATTATTTATTCTGGTGGAGCTATATCTTCAAATACCTTTATGCTAAAAAGTTATTTAGATACAGTTTCAAGAGAATTAGATGACTCAGCTAGAGTTGATGGATGTTCTAACTGA", "aa": "MVISTICVALNAYAYSRFKFAGSKHSLTIIMMLQMIPATSSLICLYIIVRMGNDLGINSILMLVIIYSGGAISSNTFMLKSYLDTVSRELDDSARVDGCSN", "aa_hexdigest": "dd98732cdd46758f0f01f06dcbe70765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.933999999987, "isoelectric_point": 7.704548072814942 }, "id": "EFPKPENHBP_1392", "locus": "GCA000186005_01379" }, { "type": "cds", "sequence": "contig_1", "start": 758541, "stop": 758672, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGTGAAAACAAAATTTATGATTCAATTGTTGATAACATTTCAACTAAAAAAGTTATCAACAAAAAAGTTCGTATCAACTATAACTTATCAGATACTAAACCTCCAACACCAATGGAAATAGTTTGA", "aa": "MNSENKIYDSIVDNISTKKVINKKVRINYNLSDTKPPTPMEIV", "aa_hexdigest": "6aaebf92ca75576726d99dd6b9b01785", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4951.672700000002, "isoelectric_point": 9.15762119293213 }, "id": "EFPKPENHBP_1393", "locus": "GCA000186005_01380" }, { "type": "cds", "sequence": "contig_1", "start": 758675, "stop": 759070, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter permease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "nt": "ATGTTAGTTACTGGTAACTTACAAGCAATTCCAAAAGATATTTATGAAGCTGCTTCTATTGATGGAGCCAAAAATTATCATAAATTCTTTAAGCTAACACTACCTTCTTTATTATTAGCAATTGCCCCAATGTTAATTGGTCAATTTGTTGGAGCATTCAACAACTTCACAACTATTAGTATCTTTAGTGGCGGAGGCCCTGCGTTTGCTAAACCAACTGCTTATCAAGAAGGTGCAACTGATATTCTAATTTCATTTATCTATAAACTAGCCACTGGTGCAGTAAAAATTGAATCTGATCAAGCTTTTGCAGCAGCTTTGACAACTCTTGCAGCTCTATTAAGTATCGCTGTAGCAGCTAGAGGATTCATTAAATCTATGACAAGGAGGGACTAA", "aa": "MLVTGNLQAIPKDIYEAASIDGAKNYHKFFKLTLPSLLLAIAPMLIGQFVGAFNNFTTISIFSGGGPAFAKPTAYQEGATDILISFIYKLATGAVKIESDQAFAAALTTLAALLSIAVAARGFIKSMTRRD", "aa_hexdigest": "7bf93e1623a5a2fd25a7600fba67b3ab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF2AB6", "query_cov": 0.916030534351145, "subject_cov": 0.8633093525179856, "identity": 0.725, "score": 169.0, "evalue": 5.23e-52, "valid": false, "product": "ABC transporter permease subunit", "uniref50_id": "UniRef50_UPI0021AF2AB6" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF2AB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "product": "ABC transporter permease subunit" }, "genes": [], "id": "EFPKPENHBP_1394", "locus": "GCA000186005_01381" }, { "type": "cds", "sequence": "contig_1", "start": 759635, "stop": 759955, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTCTTCAAACAAAAAACTAAAGGGATTCTTTTACTTTTAGTAACCACATTATTGTATGCAATTTTTATTCCTTTTAGTTTCGGAGCTTATACTACTGGTATTGATGGTATGGAAGGGATTCTGAGTTTCATTGACTTAGGAGCTCGACACTTCAATTCTAGCATGGGCATATTTAGAGATGCTCGGCGTTACCTTTTTGGTGGTGTCATATCTGTTATTATCTTAACAATAGTATTAATTTACTTTACAGTTTGTTCAATTATTGCATTTAGAACAGCTAAATTAATGGAAGAAGGGTCACGTCCAAGTAAATGA", "aa": "MIFFKQKTKGILLLLVTTLLYAIFIPFSFGAYTTGIDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFTVCSIIAFRTAKLMEEGSRPSK", "aa_hexdigest": "85875a450262fe94b1b9272598bd0912", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11855.068399999987, "isoelectric_point": 9.726554679870606 }, "id": "EFPKPENHBP_1395", "locus": "GCA000186005_01382" }, { "type": "cds", "sequence": "contig_1", "start": 760106, "stop": 760570, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86", "UniRef:UniRef90_UPI002114FE86" ], "nt": "TTGCTTAAATCTGAACTTTATTTCAAAGAATCGGCAATTTATGAAAAATATTCTTATGAAGGAAATTATAAAAAAGAATTAGCTTTAGCCTTAAAAGAAAGAGCAATAAATGCTGAGCAAGTAAGACTAAAATTCTTATATGAAAAAGTTTTAGCTATTTATGAAACAAAACTAAATTCTTTAAATCTTAGCTCAAATGAAAATTCTAACATTTTAAAAGAACAAGTTAGAAACAAAGAACAATATCAAAGCGAAAAAGAATTAGTTTCTAACAAGAAAAAACAACTTTATAATCAATATCTTGAAACAGTTAAGCAAACAGCTTTAAGATATAAAAATAAAGAAATATCAAAACAAGCTTTTAAACATAGTAAAATGGAAGCAAAGATTGATTATAATGAAAAAAGATATGAATTAAAACTTCAAACTAACAGTCTTAAAAATAAAGAAATTTTATCAACTTGA", "aa": "MLKSELYFKESAIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKVLAIYETKLNSLNLSSNENSNILKEQVRNKEQYQSEKELVSNKKKQLYNQYLETVKQTALRYKNKEISKQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILST", "aa_hexdigest": "5f33af9db1673729df1141a3ff681cf8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 759803, "stop": 760570, "observations": { "mutation": [ 760000, 760108 ], "stop": [ 760000, 760108 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002114FE86", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.948, "score": 263.0, "evalue": 2.59e-85, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.9372549019607843, "score": 463.0, "evalue": 2.72e-166, "description": "Internal stop codon at 760000, 760108. Nonsense mutation around 760000, 760108.", "psc": { "uniref90_id": "UniRef90_UPI002114FE86", "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002114FE86", "UniRef:UniRef50_UPI002114FE86" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18426.88150000001, "isoelectric_point": 9.476417350769044 }, "id": "EFPKPENHBP_1396", "locus": "GCA000186005_01383" }, { "type": "cds", "sequence": "contig_1", "start": 760571, "stop": 761647, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0", "UniRef:UniRef50_UPI002113FE78", "UniRef:UniRef90_A0A3P8K8F0" ], "nt": "ATGAAGATTAACAAAAATTTATTTTTACGAGCTAGAACAAAACTACAAGATAACTTAAAAAGAGAACTTTCAAGTTTATATGCGGCTTATTCAAATAAAATTAAAGCTATTAAAGATGCTATTAAAAAGATATCGTTTGCTGATAACGAAATCTCAATGATTAAGTATGAAATTAAAGCGTTAATAAAAGAAAAAAAGGCTCTTAAAAAATATGTACTAGAATTTCAAAAATCACTAAGACTAACAGCTGACATTGATGAAAAGAAGAATGAATTATTAGAAGAATTAAAGCAAAAAACAATTAAAGAAGAAAATAAAATTCTTTCTAAATATGCTTTATTCAATATAACTCTAAAATATCTTAAACATAATTCAGATCTTGATTTTGACATCAATAAAATTAAAAACTATTTGCATAAACAAGAACTAAAAATATTAAATACTTTAGAAGACCCAAAATCTTATTTCCAAAACTTTTATCAAAAATTGGAAAAACGTCGTTTAGAATTAATTGAAAAACGTAATTCACTAAACCACAAATATCAAAATAATAAATTTATTGAATTAAAGATTTATAAAGCAAACAAATACAACATTAAATTAGAAACAAATCAAAAAAATCTTGCTTTAGAATATGAATATAATCAAAAAGCAGAATTACAAAAACAAGAAGTAAAAGCATATAAAAAAGAAGCATATGCTAAAATCGAAGAACACAAAAATGAGATTAAACGTGTTGAAAAAGGCAATATTGAAAAAATTAAAAAAATAAAGCAAAATGGAAAATCAAAAATTAAGATTGTAAATCAAAATTTCAAGCAACAACTAAAGAAAATCGATGATTTAGTTGCTACTAGAAATTACCAACAATATTTAGAATTTTTAGCTAAAAATAACTTTATAGATTCTAATAAAGATGAATCTATAAAAATTACCAAAAAATCTGTTTTACAGTCATTTAAGAAAAATAAACAACTTGTCTATAATGACAAAAAAACATCTGCTTTAGCTAAAATATTTAAAAAATTATTCTTTGGTTTTTTTAACACAAAAAGCCTAAAAAAAGAATTTGAATGA", "aa": "MKINKNLFLRARTKLQDNLKRELSSLYAAYSNKIKAIKDAIKKISFADNEISMIKYEIKALIKEKKALKKYVLEFQKSLRLTADIDEKKNELLEELKQKTIKEENKILSKYALFNITLKYLKHNSDLDFDINKIKNYLHKQELKILNTLEDPKSYFQNFYQKLEKRRLELIEKRNSLNHKYQNNKFIELKIYKANKYNIKLETNQKNLALEYEYNQKAELQKQEVKAYKKEAYAKIEEHKNEIKRVEKGNIEKIKKIKQNGKSKIKIVNQNFKQQLKKIDDLVATRNYQQYLEFLAKNNFIDSNKDESIKITKKSVLQSFKKNKQLVYNDKKTSALAKIFKKLFFGFFNTKSLKKEFE", "aa_hexdigest": "d7271fe973ad96c0118c2cf0c95f8fe7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002113FE78", "query_cov": 0.8715083798882681, "subject_cov": 0.8167539267015707, "identity": 0.84, "score": 416.0, "evalue": 4.28e-142, "valid": false, "uniref50_id": "UniRef50_UPI002113FE78" }, "pscc": { "uniref50_id": "UniRef50_UPI002113FE78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002113FE78" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 760268, "stop": 761785, "observations": { "insertion": [ 761530 ], "deletion": [ 761448 ], "mutation": [ 760572, 761761 ], "stop": [ 760572, 761463, 761466, 761490, 761761 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F0", "query_cov": 0.9972067039106145, "subject_cov": 0.4518987341772152, "identity": 0.8540000000000001, "score": 491.0, "evalue": 6.04e-166, "gene_start": 2, "gene_end": 358, "reference_start": 48, "reference_end": 404, "reference_sequence": "MEKLKLFNWYGEEFDTILPEEQDTLKAYKHHVRNVVNRRIDKINSQKKINKNLFLRARTKLQDNLKRELSSLYASYSNKIKAIKDAIKKISFANSTISLIKYEIKALIKEKKALKKYVLEFQKSLRLTADTDEKKTELLEELKQKTIKEENEILSKYALFNITLKYLKHNPDLDFDIDKIKNHLHEQELKVLNTLEDPKSYFQNFYQKLENRRLKLIEKRNSLNHKYQNNKSIELKIYKANKYNIKLETNQKILALEYKYNHKAELQKQEVKAYKKEAYAKIEEHKNKIKRVEKDNIEKIKKIKQNGNSKIKIINQNFRQQLKKIDDLVATRNYQQYLEFLAKNNFINSNIEESKKITKKSVLQSFKKSGQLVYNDKKTSALAKIFKKLFFGFFNTKSLKKEFEWLLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.8851485148514852, "score": 796.0, "evalue": 6.34e-288, "description": "Internal stop codon at 760572, 761463, 761466, 761490, 761761. Frameshift due to insertion around 761530. Frameshift due to deletion around 761448. Nonsense mutation around 760572, 761761.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F0", "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F0", "UniRef:UniRef50_A0A3P8K8F0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 42917.5666, "isoelectric_point": 9.822805976867674 }, "id": "EFPKPENHBP_1397", "locus": "GCA000186005_01384" }, { "type": "cds", "sequence": "contig_1", "start": 762190, "stop": 762294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAATCCATACATTAAACCCAAATTAAATATAGACAAATTTAGTTTTTACTTTTTAGTTTCTTGTTTAGCAGCAATTACAGTCTTAATGATTTTTTGA", "aa": "MDQNPYIKPKLNIDKFSFYFLVSCLAAITVLMIF", "aa_hexdigest": "a0d5561bfb9bc7bbbb48c77e6151620b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3984.785399999999, "isoelectric_point": 7.898231315612794 }, "id": "EFPKPENHBP_1398", "locus": "GCA000186005_01385" }, { "type": "cds", "sequence": "contig_1", "start": 762294, "stop": 762383, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAAAAATTAACAGCAATTTACAAAGAAAAAATTGACACAATTAATAGTTCAACTGAATCGACAGCTGTTATTAGATTTGAATAA", "aa": "MYEKLTAIYKEKIDTINSSTESTAVIRFE", "aa_hexdigest": "0dc7400cd9bf7eca0ac9adff960cfd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3381.8024000000005, "isoelectric_point": 5.155035209655761 }, "id": "EFPKPENHBP_1399", "locus": "GCA000186005_01386" }, { "type": "cds", "sequence": "contig_1", "start": 762474, "stop": 762854, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTAATCAATGCAAAAATTGCTGAAGCTAAAAAAGTTTCACAACTTTTATCTAAAAAAGCAGTAGCTGATGAAGTTATTACAAAAGCAGTTAAAGCATATCTTTACGCAAGATACATTAACCAAGCTGAAGTTAGAAATCTTGGTGACAAATTAACTAAAGAATTTCAATTTCCAGCAAAAGATAAAAGCACTAAAGTGCCAGCTTCTAAAGTTATTGAAATTGTTGAACAATATAGAAAAAATCAAACAGTTGACTTAATTTATCAAACATTATCTTCAACTAAATCATTAGCTGATGGTGGATTGAATGTAATTCAATACCAACCAGGACGTATTGACCAATCAAATCCTCAATTTGGTAATGTTGCATGA", "aa": "MQDLINAKIAEAKKVSQLLSKKAVADEVITKAVKAYLYARYINQAEVRNLGDKLTKEFQFPAKDKSTKVPASKVIEIVEQYRKNQTVDLIYQTLSSTKSLADGGLNVIQYQPGRIDQSNPQFGNVA", "aa_hexdigest": "040187f514c64e9f0fa1172cff937f15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14046.941599999984, "isoelectric_point": 9.49382381439209 }, "id": "EFPKPENHBP_1400", "locus": "GCA000186005_01387" }, { "type": "cds", "sequence": "contig_1", "start": 763041, "stop": 763226, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCAGCTAACAATTTACCAGTACATGCTGTGTTAAACAGTACTTTAGAATCTAAATTAGGCATGAATTTCAAAGATATTCGTGATAAATATACTGAAAAAGGTACTGGATTACGTAATGCATTAGCAGCAATTTTAGGTTTGGAAAAACTTTCGGATTTACAAGGTAATGGCCAAACTTGA", "aa": "MYSANNLPVHAVLNSTLESKLGMNFKDIRDKYTEKGTGLRNALAAILGLEKLSDLQGNGQT", "aa_hexdigest": "9e1b85fcaf7c5daa9a61b449701ccc49", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6621.468400000001, "isoelectric_point": 8.08609218597412 }, "id": "EFPKPENHBP_1401", "locus": "GCA000186005_01388" }, { "type": "cds", "sequence": "contig_1", "start": 763266, "stop": 763379, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAATTGAAATATACAGATGCTGCTGAATTTAATAGAGATTATGATTCATCAATTCAAGTAATTAATAATTTATCAAATGTAGGCGATTCTCCAAGTGCAAGTACTTGA", "aa": "MSKLKYTDAAEFNRDYDSSIQVINNLSNVGDSPSAST", "aa_hexdigest": "cd325d09b4557dbba019b2b12b6d5bc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4039.3065000000006, "isoelectric_point": 4.359459877014161 }, "id": "EFPKPENHBP_1402", "locus": "GCA000186005_01389" }, { "type": "cds", "sequence": "contig_1", "start": 763815, "stop": 763988, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAAATTACTTTTCTTAAAAAATGGAAAAATTTCAACAGGTCTTTACACAGAAAATCCTATCCATGTTGAATTCAAAAAAGCAGTTGATTTAATGTCAGAATTCTTCTTTCCTATATATGAAGCTGCATATATCCTAGACTCAGGAGCATATGCTACTTCAGTATGA", "aa": "MMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDSGAYATSV", "aa_hexdigest": "78f115e3329eec8aa4dee78466592bf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6482.537899999999, "isoelectric_point": 6.534346961975097 }, "id": "EFPKPENHBP_1403", "locus": "GCA000186005_01390" }, { "type": "cds", "sequence": "contig_1", "start": 764040, "stop": 764426, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26", "UniRef:UniRef90_UPI0001E32F26" ], "nt": "ATGCCTACAGGGGCAACAGATGAAAAAATTGTGCCAGATATTTTTTATGCACCACATGATAGAATTACAACTTTAGTTGCATCTAATGCAGTTGCTGATTTAGATCAATTTTCACCAGGATTATTAGATCGTATTGCTAAAAAAGTTAAAGCAACTGAAGAAGAAAAGAAACAACTTAGATCATTTGGTTCAGTAGTCGGTATTGAAAAAATTACTCAATTACCAGCTGAAAAACTTTTTGGTATTAGACACAACACTGAAGGTGTTATAGTTGCTTCAACAAAATCTTTAGCAGATGTTAAAAAAGACTTAGCTAAACCTGAAAATGATACATTGCTAAAATTAGTAAAACAAGGTCGTGCATTTTTAAGATTCCAAGACTTCTGA", "aa": "MPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDF", "aa_hexdigest": "e3df7699676ab7edcd1fc9399818730e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 763784, "stop": 764669, "observations": { "insertion": [ 763841 ], "mutation": [ 764042, 764441 ], "stop": [ 764042, 764441 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32F26", "query_cov": 1.0, "subject_cov": 0.43537414965986393, "identity": 1.0, "score": 248.0, "evalue": 8.98e-81, "gene_start": 1, "gene_end": 128, "reference_start": 82, "reference_end": 209, "reference_sequence": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDVWGALEMPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDFWYGNGILGAVAHEYKAEDMMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDQEHMLLQYEAKEILNKMT" }, "paralog": false, "identity": 0.9931972789115646, "score": 560.0, "evalue": 1.63e-206, "description": "Internal stop codon at 764042, 764441. Frameshift due to insertion around 763841. Nonsense mutation around 764042, 764441.", "psc": { "uniref90_id": "UniRef90_UPI0001E32F26", "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32F26", "UniRef:UniRef50_UPI0001E32F26" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14150.149499999978, "isoelectric_point": 8.859583854675293 }, "id": "EFPKPENHBP_1404", "locus": "GCA000186005_01391" }, { "type": "cds", "sequence": "contig_1", "start": 764439, "stop": 764669, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAATTTTAAGAAAACTTTTAAAAAAATAGCAATTGGCTTTGCAACCTTAGCAGCTCCAGTGACAGCATTAGCTACAATTAGTTGTGGTACTACTGAAAGCAAAACAATTTTAATTGCAGTTGATGGCGTTCAACAAAAATTCTATGACAAAGCAATTGAATTGTTTAAGAAAACAAAATCAGGCCAAAATGGCTATGAAATTAAAACAATAGCTAAAGATGTTTGA", "aa": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDV", "aa_hexdigest": "05fdf836fc0fafb13ac8783bb023d959", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8232.566499999997, "isoelectric_point": 9.630819129943848 }, "id": "EFPKPENHBP_1405", "locus": "GCA000186005_01392" }, { "type": "cds", "sequence": "contig_1", "start": 765513, "stop": 765788, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG64", "UniRef:UniRef90_C4XG64" ], "nt": "TTGAATTTATCCAAGAATGAGAATTCGATTGAACAAGCTCATAAGAATAAAAAATCTAATTTTAATTTAGCCAAAAAAATAATTAATTATCGTAAAGAAAATCCAAAAATGTTTGCTGAAAAAGAAAATACTTATGCTAATTTAGATGATCTCTTTAAACCCGGTTATGATTATAAAGGACTTGGATATAAATTAGTTAAAGCAAAGCAAAAAGATAAAAAGTTTTTAGTTTTAGTCAATTCAATTTATTATCATACAGCTTATGGTCAAGAATGA", "aa": "MNLSKNENSIEQAHKNKKSNFNLAKKIINYRKENPKMFAEKENTYANLDDLFKPGYDYKGLGYKLVKAKQKDKKFLVLVNSIYYHTAYGQE", "aa_hexdigest": "65d3d91ba46794b1bd58859ead91a0e1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 765387, "stop": 765809, "observations": { "mutation": [ 765515 ], "stop": [ 765515 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG64", "query_cov": 1.0, "subject_cov": 0.65, "identity": 0.9890000000000001, "score": 180.0, "evalue": 8.23e-57, "gene_start": 1, "gene_end": 91, "reference_start": 8, "reference_end": 98, "reference_sequence": "METSHIFLNLSKNENSIEQAHKNKKSNFNLAKKIINYRKENPKMFAEKENTYANLDDLFKPGYDYKGLGYKLVKAKQKDKKFLVLVNSIYYHTAYGQEWNLVFKNAPTKINVIFSNNSSINNEKITFSGNTGLMLVELTY" }, "paralog": false, "identity": 0.9857142857142858, "score": 279.0, "evalue": 3.08e-98, "description": "Internal stop codon at 765515. Nonsense mutation around 765515.", "psc": { "uniref90_id": "UniRef90_C4XG64", "uniref50_id": "UniRef50_C4XG64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG64", "UniRef:UniRef50_C4XG64" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG64" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10713.135499999997, "isoelectric_point": 9.589752769470213 }, "id": "EFPKPENHBP_1406", "locus": "GCA000186005_01393" }, { "type": "cds", "sequence": "contig_1", "start": 765854, "stop": 766048, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGGAGTTAAAACTTTTTATCTCGAAATTAAGCAAAATGATTTTAAAGTTTTAAATGTAATGAGTTATGGATTAAGTAACCTATTAATTCAAAGTGGCAATCCAATTCTTTATAATGGTAATGAATTAAATATGAGAGGTGGCCCACAAGCACATTCTGATGCCTTTTTAAGAGAAGCTTTTAAATGA", "aa": "MIFGVKTFYLEIKQNDFKVLNVMSYGLSNLLIQSGNPILYNGNELNMRGGPQAHSDAFLREAFK", "aa_hexdigest": "02683061485069b064bc45f3d1003325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7254.2836, "isoelectric_point": 8.085898780822756 }, "id": "EFPKPENHBP_1407", "locus": "GCA000186005_01394" }, { "type": "cds", "sequence": "contig_1", "start": 766304, "stop": 766453, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAGAAAATAAACATGAATTTAAAGACGCAACAGGCTTAGAAAAGAGAAAATTATTTAATGAGCAAAGATTAGCCTTAAGAGAAGTAATAAAAGAAACTAAAGATCAAAGATCAAGCGATGATATATTTTTCTTTGGTGAATGA", "aa": "MNSENKHEFKDATGLEKRKLFNEQRLALREVIKETKDQRSSDDIFFFGE", "aa_hexdigest": "58a91c581e03181e6b7760ec7eedf752", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.4847, "isoelectric_point": 5.796065330505371 }, "id": "EFPKPENHBP_1408", "locus": "GCA000186005_01395" }, { "type": "cds", "sequence": "contig_1", "start": 766781, "stop": 767119, "strand": "-", "frame": 3, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "nt": "TTGTCTTATGAAGGTGAATTTAAAAATGCAAAATTTATTGTGCCTTTTAACAAATCTATTAAGCCTTCAAATGTTATGTATCAATTAACAATTTATTCTTTTGCAGATGGCAATAATGATGGTATTGGCGACTTTATTGGACTAAATGAAAATTTAGAATATTTTAGCAATTTAGGAATTGATACTTTATATCTTTCACCAATACATCCAGCCAGCTCATATCATGGCTATGATGTAATTGATTATACAGATGTTGCTCCTGAATTAGGTGGCATGGAAGCCTTTGATCAATTTTTAATAAATGCCCATAAAAAAGGCATAAAAGTTATTATGGATTGA", "aa": "MSYEGEFKNAKFIVPFNKSIKPSNVMYQLTIYSFADGNNDGIGDFIGLNENLEYFSNLGIDTLYLSPIHPASSYHGYDVIDYTDVAPELGGMEAFDQFLINAHKKGIKVIMD", "aa_hexdigest": "92899d05716cb1b7e7f0ea738b36f460", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF384D3", "query_cov": 0.9285714285714286, "subject_cov": 0.832, "identity": 0.779, "score": 172.0, "evalue": 9.51e-54, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI002FF384D3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF384D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "EFPKPENHBP_1409", "locus": "GCA000186005_01396" }, { "type": "cds", "sequence": "contig_1", "start": 767161, "stop": 767262, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3", "UniRef:UniRef50_A0AB32XCK3", "UniRef:UniRef90_A0AB32XCK3" ], "nt": "ATGAGTAAAAAAACAAGAAAAAAATTAGCATATTTTTCTTTAATTGCACCTATCGCTTTATGTTTAAGTGCAAGTTGCACAAGTGTTAAAAAAGATTATTAA", "aa": "MSKKTRKKLAYFSLIAPIALCLSASCTSVKKDY", "aa_hexdigest": "0d0991061a8fb19f63031ca6f39809b5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59D51", "uniref100_id": "UniRef100_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCK3", "uniref90_id": "UniRef90_A0AB32XCK3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCK3", "uniref50_id": "UniRef50_A0AB32XCK3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCK3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3666.4218999999994, "isoelectric_point": 9.835119438171386 }, "id": "EFPKPENHBP_1410", "locus": "GCA000186005_01397" }, { "type": "cds", "sequence": "contig_1", "start": 768427, "stop": 768984, "strand": "-", "frame": 1, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5", "UniRef:UniRef90_C4XG67" ], "nt": "ATGCAAAAAAATGAGAATTTTACCAATAATATTGCAGCCAATGAAGATGACAAATCTTTTAATGAATTATCATTTGGCAAAAAGTTTAAGCAACACTTCGCTAAGTCATTTCGTCTTAGTGTTTTAGATATTGCTTTAGCAGGTATATTATTGGCAATTCATTTTGTTGTTGCAATAATTTCTAAGTTTACAATTTTAAAAATAATACCCTTAGAAACTGATGCAATTTTTTTTATATTATATGGAATCTTTTTTGGACCACTTAAAGGATCTTTAATATCATTTTTAGCTGATACTTTTATGATGCTTTTAACTGGTACAATTGGAACTTGGTATTATCTTTATGCATTGATTCCAATAGGCATTTGTATTTTAAGCTCCTTTTATTACTTTTTTTATCGTGCTAGTTTTTTATTTAGAATTATTTTTCCAATAATTGTAATTTCAGTTGGTTTCGCTTTATTACTCTATGTTGTTATAAATAGTGGAAATGATAAAGGTTATCATATTAAAGGAATTAAGAAGCTAAAATATTTACCTAAAAATGCAGTTTTATGA", "aa": "MQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVL", "aa_hexdigest": "a94329f6d0f86e7ade84262bce6e0fec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 768124, "stop": 768996, "observations": { "mutation": [ 768276, 768333, 768429 ], "stop": [ 768276, 768333, 768429 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG67", "query_cov": 1.0, "subject_cov": 0.589171974522293, "identity": 1.0, "score": 343.0, "evalue": 2.58e-117, "gene_start": 1, "gene_end": 185, "reference_start": 5, "reference_end": 189, "reference_sequence": "MIQNMQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVLWMLLIYFNFSVIAFIFIIFQLLFAKNKKVKKRWFDYLFVFSIIIFIMIIFRWLIGPYVYINYYNYLYAGRKSLNYTGEHYYLITIPILLKSLITIPIYSIIMNPIFSVAILLKEKFVEKNSKISY" }, "paralog": false, "identity": 0.9862068965517241, "score": 535.0, "evalue": 2.52e-195, "description": "Internal stop codon at 768276, 768333, 768429. Nonsense mutation around 768276, 768333, 768429.", "psc": { "uniref90_id": "UniRef90_C4XG67", "product": "ECF transporter S component", "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG67", "UniRef:UniRef50_A0A3P8LHP5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5" ], "product": "G-protein coupled receptors family 1 profile domain-containing protein" } }, "id": "EFPKPENHBP_1411", "locus": "GCA000186005_01398" }, { "type": "cds", "sequence": "contig_1", "start": 768997, "stop": 770076, "strand": "+", "frame": 1, "gene": null, "product": "Phosphomannomutase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68", "UniRef:UniRef90_C4XG68" ], "nt": "ATGTTAAAAATTTTTATTTGTTATTATATTATATATATTATGAACAAAAAATTAGAATTTGGAACAGCTGGAATTAGAGGAATTTTAGGTTCTGGTGAAGACCATTTAAATGTAGCTCATGTTCAAAGAGTTATTCATGGTTTTGCTAAATATTTAAAAAACAAATATAGCCACATTAATCCAATTACTGTTGTTATTGGACGTGATAACCGTAGATTCTCAGCTACATTTGCAACCTTAAGTGCTCAAATTTTAAGCCAATATGACATTAAGGTTATTTTTCCTCTATACTTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGA", "aa": "MLKIFICYYIIYIMNKKLEFGTAGIRGILGSGEDHLNVAHVQRVIHGFAKYLKNKYSHINPITVVIGRDNRRFSATFATLSAQILSQYDIKVIFPLYLTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFK", "aa_hexdigest": "336e5ae5457fab16cff26d42d6dfea7c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 768922, "stop": 770379, "observations": { "mutation": [ 770074 ], "stop": [ 770074 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG68", "query_cov": 1.0, "subject_cov": 0.6468468468468469, "identity": 1.0, "score": 719.0, "evalue": 4.59e-259, "gene_start": 1, "gene_end": 359, "reference_start": 26, "reference_end": 384, "reference_sequence": "MKRFVIFIGCNIIGKILIFLHILNYMLKIFICYYIIYIMNKKLEFGTAGIRGILGSGEDHLNVAHVQRVIHGFAKYLKNKYSHINPITVVIGRDNRRFSATFATLSAQILSQYDIKVIFPLYLTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFKWIGMIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDSWLALRPSGTEPKIKFYIFAFGKDQQEAQAKFDNFARIIKEIK" }, "paralog": false, "identity": 0.9958762886597938, "score": 966.0, "evalue": 0.0, "description": "Internal stop codon at 770074. Nonsense mutation around 770074.", "psc": { "uniref90_id": "UniRef90_C4XG68", "product": "Phosphomannomutase", "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG68", "UniRef:UniRef50_C4XG68" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68" ], "product": "Phosphomannomutase" } }, "id": "EFPKPENHBP_1412", "locus": "GCA000186005_01399" }, { "type": "cds", "sequence": "contig_1", "start": 770083, "stop": 770463, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGACGATTCTTGA", "aa": "MIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDS", "aa_hexdigest": "1f68d738d7186442c746fb060d112ead", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 57, "aa_cov": 0.4523809523809524, "hmm_cov": 0.9649122807017544, "evalue": 1.2821035474960183e-06, "score": 24.884675979614258, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14355.937999999982, "isoelectric_point": 4.472285270690917 }, "id": "EFPKPENHBP_1413", "locus": "GCA000186005_01400" }, { "type": "cds", "sequence": "contig_1", "start": 770657, "stop": 770851, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAA", "aa": "MNFLKSPLIDYFSSTYLVIKLPEIPITGLTEIHNLVSKVFKSRIMLRVSKRSIVVTTLYVFKPE", "aa_hexdigest": "67dff05b1e79d9cedba223846d52d62f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7415.8406, "isoelectric_point": 9.89874973297119 }, "id": "EFPKPENHBP_1414", "locus": "GCA000186005_01401" }, { "type": "cds", "sequence": "contig_1", "start": 770884, "stop": 771156, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATAACATTCTTAATCATTTTTGGTTTTGCTCAACCACATCATAACTATGCAAAAATTTTAACAAATGGTGTTGGAACTCAAATTATTGAAGATTCAGGTAACAGAGTATTCTTCGGAATGAGAGAATTTACAACATTCTTCTATATATTGGTTGTTAACTTCTTAATTAACTTTATTTATCCTAAATATAAAAAAGTTGCAGTTGAAATTTCTTGTGGGCCTTCACCAGATTTAGTGTTAGCGTACTTTAAGAAAATTAACTATTGA", "aa": "MTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINY", "aa_hexdigest": "1ec7a4f6836d5d243dfd1b43fbe04920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10396.10749999999, "isoelectric_point": 9.13157596588135 }, "id": "EFPKPENHBP_1415", "locus": "GCA000186005_01402" }, { "type": "cds", "sequence": "contig_1", "start": 771406, "stop": 771957, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69", "UniRef:UniRef90_C4XG69" ], "nt": "ATGAATAACAACGAAAAAGATACTACCAAACTAGAACAAGTGCATAACCTTGAAGTTAGTAAAGAAGCTATTCAAGAAGTTAAAGATATGGTTTTTGAAGCAAAAAATCAAATCCAAGAACCTAAAAAGGAAATTCTTGATGAAAAATCTCTTGACAGTCTTGAAATGGTCCGTCATAATTATGAAAAAGATAATCCTAAAAACAAGGAAGTTAGGAAAAGAATTGCTAAACTCTATGCTCGTCGTTTAGTTTTTATCTTTTTATCAGCATTGGTCTTTAACTTTGGTGTGCAAGTATTCTTAAATAGAGGTGAAACTATTCCTAGTGGACTTAGTGGAGCTCCCTTATTAATTAACTTAATTGCACCGAGTACTAAGCCTTATTTTGCTTTAATTTACTTAGCTTTTAACTTACCGCTTCTTTTAGGATTTGGATTTAAGATTAAGAAAAGTTTTACTTTGATGACACTAGCTTTTATGCTATTTCAAATATTAACTAATTTGATATTAACTTCAGTTCCAATTGGAGAAAATAAAATAGTTTTAGACTGA", "aa": "MNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLD", "aa_hexdigest": "3c08ae7db96ca4ca29606584b3feb53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 771103, "stop": 771966, "observations": { "mutation": [ 771261, 771324, 771375, 771408 ], "stop": [ 771261, 771324, 771375, 771408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG69", "query_cov": 1.0, "subject_cov": 0.4295774647887324, "identity": 1.0, "score": 321.0, "evalue": 8.24e-107, "gene_start": 1, "gene_end": 183, "reference_start": 4, "reference_end": 186, "reference_sequence": "MNIMNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLDWLHEHINFAPGWTKEIKVGSVTLENPNSWPIFAYGAIGSGCAGTAIAIAWKNGGSTGGTDIVAYYFSTKKQKSVGNILSLISIIMTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINYWHGYTVVEGISGYSGLKTYSVVTTMLLLETRSIIRDLKTLDTKLWISVKPVIGISGSFITKYVDEK" }, "paralog": false, "identity": 0.9825783972125436, "score": 527.0, "evalue": 2.09e-190, "description": "Internal stop codon at 771261, 771324, 771375, 771408. Nonsense mutation around 771261, 771324, 771375, 771408.", "psc": { "uniref90_id": "UniRef90_C4XG69", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG69", "UniRef:UniRef50_C4XG69" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69" ], "product": "ABC transporter permease" } }, "id": "EFPKPENHBP_1416", "locus": "GCA000186005_01403" }, { "type": "cds", "sequence": "contig_1", "start": 772102, "stop": 772401, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02888", "RefSeq:WP_013354830.1", "SO:0001217", "UniParc:UPI0001A8C1DC", "UniRef:UniRef100_C4XG70", "UniRef:UniRef50_A9NF54", "UniRef:UniRef90_C4XG70" ], "nt": "ATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGCCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAACCCTAAATCAACTCATAAGAGAAAATTGGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAG", "aa": "MIAIIETGGKQILVKEGQTIFIEKIEGKEGSTVDFDKVLLIDNKIGKPYLKGAKVTGKIQKQGKAKKIVVYRHNPKSTHKRKLGHRQPYTRVEITKIVG", "aa_hexdigest": "912f8497cc2d988986112d18fe7b9be6", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1DC", "ncbi_nrp_id": "WP_013354830.1", "uniref100_id": "UniRef100_C4XG70", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DC", "RefSeq:WP_013354830.1", "UniRef:UniRef100_C4XG70" ] }, "ips": { "uniref100_id": "UniRef100_C4XG70", "uniref90_id": "UniRef90_C4XG70" }, "psc": { "uniref90_id": "UniRef90_C4XG70", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_A9NF54", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A9NF54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NF54" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "EFPKPENHBP_1417", "locus": "GCA000186005_01404" }, { "type": "cds", "sequence": "contig_1", "start": 772411, "stop": 772809, "strand": "+", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71", "UniRef:UniRef50_A0A3P8MF53", "UniRef:UniRef90_C4XG71" ], "nt": "ATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCGGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAA", "aa": "MAHTKAGGSTRNGRDSRGQRLGIKLGDGQFCTAGSIIFRQRGTKIFPGTNCGRGNDDTLFALITGYVKFETRRNRTYASVYPERVSSALSQPVVKKAAVKKETAPKVAVKKPAAKKATTTTKKVSAAKKATK", "aa_hexdigest": "9de227b38602171f777e5432384a2e2f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C1DD", "uniref100_id": "UniRef100_C4XG71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71" ] }, "ips": { "uniref100_id": "UniRef100_C4XG71", "uniref90_id": "UniRef90_C4XG71" }, "psc": { "uniref90_id": "UniRef90_C4XG71", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_A0A3P8MF53", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF53" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "EFPKPENHBP_1418", "locus": "GCA000186005_01405" }, { "type": "cds", "sequence": "contig_1", "start": 772988, "stop": 773167, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGA", "aa": "MLFQVISFLNKSTDSSTLKEELVIFEYTSPYKQLIVIPAIVAAAKMIKIVPKTQFFLAI", "aa_hexdigest": "79b1208bcc328e84e83f8063087d7015", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6678.974700000001, "isoelectric_point": 9.158330345153807 }, "id": "EFPKPENHBP_1419", "locus": "GCA000186005_01406" }, { "type": "cds", "sequence": "contig_1", "start": 773146, "stop": 773352, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCGCAGTTGCTATTTCAATGAAGGATGGATTTGCTGCTTGTAAAGAATTTATAAATAACATTTTTCATAATTCAACTTCAATTTCATTAATTGGTGGCTCAATTATTGCTTTAGTTGCAATTGCTTTATTCTTTATTCTTTCAACAGCTACAGTCATTGTTTACTTTGTAACATCAATAGCTCATATAGCAAGAAAGAATTGA", "aa": "MCAVAISMKDGFAACKEFINNIFHNSTSISLIGGSIIALVAIALFFILSTATVIVYFVTSIAHIARKN", "aa_hexdigest": "1464c39fe1539d6b33bed806147b9515", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7250.568299999998, "isoelectric_point": 8.80420551300049 }, "id": "EFPKPENHBP_1420", "locus": "GCA000186005_01407" }, { "type": "cds", "sequence": "contig_1", "start": 773356, "stop": 773514, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAGTAGAACTAAATTTATTCCGCTAATAGTATTATTAGCTTTTGTAGTTGGCTTTTGTTTATTGTTCATTTTTGGTATGCAACCTGAAATGATCATCAAAGAAGAAGCCAAACCAGCAACAACTGAATTTGGAGTAGGATTCTTTGGCACTTGA", "aa": "MYSRTKFIPLIVLLAFVVGFCLLFIFGMQPEMIIKEEAKPATTEFGVGFFGT", "aa_hexdigest": "10b16adc0eb81f538f845a036654f133", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5850.026299999999, "isoelectric_point": 6.047066307067872 }, "id": "EFPKPENHBP_1421", "locus": "GCA000186005_01408" }, { "type": "cds", "sequence": "contig_1", "start": 773634, "stop": 773840, "strand": "-", "frame": 2, "gene": null, "product": "Alcohol dehydrogenase 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "nt": "TTGTTCGAAAAAGAATTAGCTGGATCAATCGTTGGTACAAGACTTGATTTACAAGAATCACTTCAATATGCTGCAGAAGGAAAAGTTAAATCAGAAGTTACAAAAGTTATTAAACTAGAAGAAGTTTCAGAAGTATTTGAAAAACTTCAAAAAGGTGAATTCTTAGGACGTGCTGTTATTGACTTTGGTTACAGAGGAAAGAAATAA", "aa": "MFEKELAGSIVGTRLDLQESLQYAAEGKVKSEVTKVIKLEEVSEVFEKLQKGEFLGRAVIDFGYRGKK", "aa_hexdigest": "cd2ec2b1850c9fbed62381028544c82f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P3G6", "query_cov": 0.8823529411764706, "subject_cov": 0.9523809523809523, "identity": 0.8, "score": 95.5, "evalue": 1.12e-24, "valid": false, "product": "Alcohol dehydrogenase 1", "uniref50_id": "UniRef50_A0A3B0P3G6" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P3G6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "product": "Alcohol dehydrogenase 1" }, "genes": [], "id": "EFPKPENHBP_1422", "locus": "GCA000186005_01409" }, { "type": "cds", "sequence": "contig_1", "start": 773847, "stop": 774188, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGTTGTTATCGGTGTTGGTGGACTTGGCCAATTAGCAATTCAATATGCTAAAGCAATGGGATATAGACCTATTGGTATCGACTTAACAGATGAAAAATGCGAATTAGCTTTAAAATCAGGAGCAGAATTTGCATTCAACTCTAAGAAAGATCCAAACTTTATTCAAAAACTTATTGAAAAGACTGGTGGTGGAGCTCACGCTGTAATTAACACATCAGTTGCTACAATTGCTGGTGAACAAGGCATGGAAATGCTTCGTCGTGGAGGACGTCAAGTATTAGTAGGCCTTCCATCAAAAGACAAACTTGGTAAAGACAACATTGGCGTTTCAGTATTCTGA", "aa": "MVVIGVGGLGQLAIQYAKAMGYRPIGIDLTDEKCELALKSGAEFAFNSKKDPNFIQKLIEKTGGGAHAVINTSVATIAGEQGMEMLRRGGRQVLVGLPSKDKLGKDNIGVSVF", "aa_hexdigest": "711b2e3fb273a542520c76c9ad915162", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 95, "aa_cov": 0.831858407079646, "hmm_cov": 0.9894736842105263, "evalue": 1.075632094541473e-25, "score": 86.43392181396484, "start": 8, "stop": 101 } ], "seq_stats": { "molecular_weight": 11905.738499999992, "isoelectric_point": 9.130608940124514 }, "id": "EFPKPENHBP_1423", "locus": "GCA000186005_01410" }, { "type": "cds", "sequence": "contig_1", "start": 774189, "stop": 774359, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCTTACACATTTGATGGATCATTTGCTGAATATGCAATTGGACACGAAGATTATGTAGGAATTGTTCCAGAAGGATTAGATTTAATTACTGGAGCACCTGTAGTATGTGCTGGTGTTACAACCTATAAGGCAATTAAAAGAGCTAAAGCTCGTCCAGGTTACTGA", "aa": "MSAYTFDGSFAEYAIGHEDYVGIVPEGLDLITGAPVVCAGVTTYKAIKRAKARPGY", "aa_hexdigest": "7bc716c17e1ab59e296874f5d62d1db6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5941.698300000004, "isoelectric_point": 5.563707542419433 }, "id": "EFPKPENHBP_1424", "locus": "GCA000186005_01411" }, { "type": "cds", "sequence": "contig_1", "start": 774651, "stop": 774755, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATAAAACATTAAAAAATTACTATTATTTTTTTAAGGAGATAGATTTTATGGCAAAAACAACTATGAAAGCTTTTGTTGTTGAAAAACCAAAACAATGA", "aa": "MFYKTLKNYYYFFKEIDFMAKTTMKAFVVEKPKQ", "aa_hexdigest": "d2de4e8ac69d91b8acd5697a4390473f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4280.078300000001, "isoelectric_point": 9.574538230895993 }, "id": "EFPKPENHBP_1425", "locus": "GCA000186005_01412" }, { "type": "cds", "sequence": "contig_1", "start": 775029, "stop": 775319, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_1426", "locus": "GCA000186005_01413" }, { "type": "cds", "sequence": "contig_1", "start": 775385, "stop": 775942, "strand": "+", "frame": 2, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "EFPKPENHBP_1427", "locus": "GCA000186005_01414" }, { "type": "cds", "sequence": "contig_1", "start": 776319, "stop": 777050, "strand": "+", "frame": 3, "gene": null, "product": "DNA processing protein", "db_xrefs": [ "RefSeq:WP_013527027.1", "SO:0001217", "UniParc:UPI0001F59832", "UniRef:UniRef100_C4XFY3", "UniRef:UniRef50_C4XFY3", "UniRef:UniRef90_C4XFY3" ], "nt": "ATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAAGAAAAAAATTAATCAAGCAGAAATAGATATAGTATTAAAGGAGCTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACGAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAA", "aa": "MNELLVYLSYKYEGNIFEIYKFLKNKKKINQAEIDIVLKELEEKGIKYITALDENFPEILKNFRYSPYVLFYKGNIGLLNKKAVCLTGDIESPIVDINIKKSQNTFLENYPLITNDFKNLDQKFVEIYRQNKKEIIHVLPYGLDYIKETINLDTELYISQYPPQTHAKLFRFKERNILVSFLAEFLIVYSSKSDSGILSLATAFANNNKEVYCYPGLSYDDGNNYLIKLGANLITQIAEVQYY", "aa_hexdigest": "ccab20775a6ba2f960cb48801231d001", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59832", "ncbi_nrp_id": "WP_013527027.1", "uniref100_id": "UniRef100_C4XFY3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59832", "RefSeq:WP_013527027.1", "UniRef:UniRef100_C4XFY3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY3", "uniref90_id": "UniRef90_C4XFY3" }, "psc": { "uniref90_id": "UniRef90_C4XFY3", "product": "DNA processing protein", "uniref50_id": "UniRef50_C4XFY3" }, "pscc": { "uniref50_id": "UniRef50_C4XFY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY3" ], "product": "DNA processing protein" }, "genes": [], "id": "EFPKPENHBP_1428", "locus": "GCA000186005_01415" }, { "type": "cds", "sequence": "contig_1", "start": 777047, "stop": 777187, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAAAAAATGCTCACATTGGTTTTGCAATGGGAAATGCCACTGAAGAAGCAAAAGTAGCTGCCGATAGAGTTATAGGTTCTAATTTAACTGGCGCTATTGGTCAAGCAATTAATGAAATTTTAGACGACAAAAATTAA", "aa": "MLKNAHIGFAMGNATEEAKVAADRVIGSNLTGAIGQAINEILDDKN", "aa_hexdigest": "3a66b63781eb15e149a092db13a4386e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 38, "aa_cov": 0.8260869565217391, "hmm_cov": 1.0, "evalue": 8.36400891165543e-09, "score": 31.815629959106445, "start": 1, "stop": 38 } ], "seq_stats": { "molecular_weight": 4783.357, "isoelectric_point": 4.950130653381346 }, "id": "EFPKPENHBP_1429", "locus": "GCA000186005_01416" }, { "type": "cds", "sequence": "contig_1", "start": 777272, "stop": 777406, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTAAGAAATCCATTAATTACTGCAGCAGAAACCACAAAGTATTTTCAAAATAAATTAAAAGATAAACAAAAAGTTTTCTTAACAAACTCAATTTACACTGATGTTAATCCTTTAGGTGTAACTAAGTGA", "aa": "MALRNPLITAAETTKYFQNKLKDKQKVFLTNSIYTDVNPLGVTK", "aa_hexdigest": "abd291e4e154f62124e4ba317619ee8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5014.794700000002, "isoelectric_point": 9.777162361145017 }, "id": "EFPKPENHBP_1430", "locus": "GCA000186005_01417" }, { "type": "cds", "sequence": "contig_1", "start": 777545, "stop": 777895, "strand": "-", "frame": 3, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "EC:3.1.3.104", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_C4XFY4", "UniRef:UniRef90_A0A3P8KLL0" ], "nt": "ATGCAAAACAAAAGAAAAGTGTTTGCTTTTGACTTGGATGGCACATTATTAACAGATGGAGTTAATACTCATCCAGAAACTCAAGAAGCAATTTTAAAATCATATAATTTAGGACATGCAAATATCATTTGTACTGGTCGTGGTTTGCAAAAAACTTTACCACTTTTAAAATCAATTAAAGGCATTAATTATTTTGTATGTTCAAATGGTGCATTATTTTATGATGTTGAAAAAGATTATTATAAAGTTTTAGGCGAAGTTAATAAAGATATATTTAATGATCTTTTTGATTATGCTCAAGCTCATGATTTAATTTTAACTATTGATACTCCTGAATTTAATGGTGCATGA", "aa": "MQNKRKVFAFDLDGTLLTDGVNTHPETQEAILKSYNLGHANIICTGRGLQKTLPLLKSIKGINYFVCSNGALFYDVEKDYYKVLGEVNKDIFNDLFDYAQAHDLILTIDTPEFNGA", "aa_hexdigest": "0a4025e14158ec0fc81e5f56f09c34d2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 777242, "stop": 777895, "observations": { "mutation": [ 777274, 777511, 777547 ], "stop": [ 777274, 777511, 777547 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KLL0", "query_cov": 1.0, "subject_cov": 0.4128113879003559, "identity": 0.845, "score": 207.0, "evalue": 4.37e-65, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MSKKRIVFAFDLDGTLLTDGVNAHPETQDAIIKSYNQGHANIICTGRGLQKTLPLLKTIKGINYFVCSNGALFYDVEKDYYRVLGEVDKSIFNELFDYANTNNLILTIDTPDFNGSWSKNSDKNILPEWVSKQNKLSDKNPHFLKPLSALTSVLNNPESKITQMAIRNPLDSAQETTKHFQGRLKGKQKVFLTNSIYTDVNPLHTTKWYGLKTLLDYLKIDTHELYCFGDSGNDIEMLQNAHIGYAMGNGTNDAKLAADKIIGSNLTGAIGQVINEILEKN" }, "paralog": false, "identity": 0.7695852534562212, "score": 336.0, "evalue": 2.31e-118, "description": "Internal stop codon at 777274, 777511, 777547. Nonsense mutation around 777274, 777511, 777547.", "psc": { "uniref90_id": "UniRef90_A0A3P8KLL0", "product": "COF family HAD hydrolase protein", "ec_ids": [ "3.1.3.104" ], "uniref50_id": "UniRef50_C4XFY4", "kegg_orthology_id": "K21064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KLL0", "EC:3.1.3.104", "UniRef:UniRef50_C4XFY4", "KEGG:K21064" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY4" ], "product": "COF family HAD hydrolase protein" } }, "id": "EFPKPENHBP_1431", "locus": "GCA000186005_01418" }, { "type": "cds", "sequence": "contig_1", "start": 778212, "stop": 778586, "strand": "-", "frame": 2, "gene": "ecfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "db_xrefs": [ "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_C4XFY5", "UniRef:UniRef90_C4XFY5" ], "nt": "ATGATTATGGTTACTTCTTTATTATTGATTACAACCAAACCAATACTACTTACTAAAGCACTTGAAGACTTGATGTTTCCATTTAAACTTCTATTCATTCCAACTCACATAATTGCTATGATTATTTCGATTGCATTAAGATTTATTCCTACTCTTTTTGAAGAAGCACAAAGAATAATGAAAGCTCAAGCAAGTCGTGGAGTTGACTTTAAGAATGGAAAAATTAAAGAAAAAGTTAAATCATTTACAACATTAATTATTCCATTGTTTGTTACTTCTTTTGCTAAAGCGGAAGACTTATCAAATGCTATGGAAACTAGGGGTTATGATCCTTATGCAAAAAGAACAAGATATCGTAAATTAAGAATAGGTTGA", "aa": "MIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIG", "aa_hexdigest": "569452122d6e589593b0f7f8381d720c", "start_type": "ATG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 778095, "stop": 778886, "observations": { "mutation": [ 778124, 778214, 778664 ], "stop": [ 778124, 778214, 778664 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY5", "query_cov": 1.0, "subject_cov": 0.4290657439446367, "identity": 1.0, "score": 228.0, "evalue": 2.51e-73, "gene_start": 1, "gene_end": 124, "reference_start": 127, "reference_end": 250, "reference_sequence": "MMKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIPWGGHSEYALTLEALARTGALIIRIYIMIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIGWLDILFFILIVGLILFVAFSQSAKYDFLPFWYKFTYFKY" }, "paralog": false, "identity": 0.9885931558935361, "score": 496.0, "evalue": 3.19e-181, "description": "Internal stop codon at 778124, 778214, 778664. Nonsense mutation around 778124, 778214, 778664.", "psc": { "uniref90_id": "UniRef90_C4XFY5", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "uniref50_id": "UniRef50_C4XFY5", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY5", "UniRef:UniRef50_C4XFY5", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY5" ], "product": "Energy-coupling factor transporter transmembrane protein EcfT" } }, "id": "EFPKPENHBP_1432", "locus": "GCA000186005_01419" }, { "type": "cds", "sequence": "contig_1", "start": 778662, "stop": 778961, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCTCCTATTGGTCGTTATTATCCACATGAAACAATAATTCATAAAATTGATCCAAGGTTAAAATTGATAATCAATATTATTTATATTGTTTTAACTTTTATTGTTAATTACTTTGTCTCTTTAGCTGTTTTATTAGTACCTCTAATGATTGCATTTGTTATTGGTTCAAGAAAAATATATCCTTTATTTAGATTAATGATTATGCCTCTAATTATTGGTTTCTTTATTCTCTTTGTTAATATCTATACTATGAAGTTAAAACCTGACTCGGACTATACAAGAATAATACCCTGA", "aa": "MKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIP", "aa_hexdigest": "7a9ac08d0e5979782e99b999bd27eeb6", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11645.265499999985, "isoelectric_point": 9.9275671005249 }, "id": "EFPKPENHBP_1433", "locus": "GCA000186005_01420" }, { "type": "cds", "sequence": "contig_1", "start": 778961, "stop": 779524, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "nt": "TTGTTTGAATCTACTATTAAAGATGATATTGCTTTTGGACCAAGAAGCTTTGGTGTGCATAAAACTGAAGCTTATGAAAAAGCTAAAAAATATCTTGAGTTAGTAGGTTTGGATGAAAGTTACTTGAATAAATCACCTTTTGGTTTAAGTGGTGGTCAAAAAAGGCGTGTTGCTTTAGCTGGAATTTTAGCTATGGAACCCGACATTTTAATTGCTGATGAACCAACAGCTGGATTAGATCCAGTTGGAGTTAGAGAAGTTTTAGAAATTTTTAATAAACTTCATCAACAAGGTAAAACGGTTATTATTATTACACATGATTTGGATAATGTTTTAGAAGTAGCAAAAAGAGTATTAATTTTTAAAGATGGAAAAGTTGTTAAAGATGGTGATACTTATGAAGTATTAAGGAATACAAAATTCTTAAAAGAAAACAATATGGAACCTCCAAAATTATTAGAATTCGTTTCTAAATTAGAGGAAAGAGGCATTAGTGTGCCTAAAGTTACATCTATTGATGAACTAGCTGCATTCTTAAATAAAAGAATGAAAGGAGGTGTATAA", "aa": "MFESTIKDDIAFGPRSFGVHKTEAYEKAKKYLELVGLDESYLNKSPFGLSGGQKRRVALAGILAMEPDILIADEPTAGLDPVGVREVLEIFNKLHQQGKTVIIITHDLDNVLEVAKRVLIFKDGKVVKDGDTYEVLRNTKFLKENNMEPPKLLEFVSKLEERGISVPKVTSIDELAAFLNKRMKGGV", "aa_hexdigest": "4c0a8bb572a8e863dcff576ff069b0c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003084074B", "query_cov": 0.983957219251337, "subject_cov": 0.8598130841121495, "identity": 0.62, "score": 241.0, "evalue": 3.87e-78, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI003084074B" }, "pscc": { "uniref50_id": "UniRef50_UPI003084074B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1434", "locus": "GCA000186005_01421" }, { "type": "cds", "sequence": "contig_1", "start": 779693, "stop": 779872, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAAATAAAAGTTAAAGATTTAGAACATATTTTTAGTCGTAGAACTCCTATGGAATTTAAAGCATTAAGTGGTATTAATGTAACAATCAATCAAGGCGAATATATAGGAATAATTGGACAAACTGGCTCAGGTAAAACTACATTCATAGAGCATTTAAATGCTCTTATTTTGCCATGA", "aa": "MQIKVKDLEHIFSRRTPMEFKALSGINVTINQGEYIGIIGQTGSGKTTFIEHLNALILP", "aa_hexdigest": "9a071ab99d2d6dc20b15bca2aa03d263", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.576271186440678, "hmm_cov": 1.0, "evalue": 1.2134239623634057e-08, "score": 31.96434783935547, "start": 23, "stop": 56 } ], "seq_stats": { "molecular_weight": 6570.593699999998, "isoelectric_point": 8.181505393981933 }, "id": "EFPKPENHBP_1435", "locus": "GCA000186005_01422" }, { "type": "cds", "sequence": "contig_1", "start": 779863, "stop": 780660, "strand": "-", "frame": 1, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_013527030.1", "SO:0001217", "UniParc:UPI0001F59835", "UniRef:UniRef100_A0AB32XCI8", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_C4XFY7" ], "nt": "ATGATTAAAGTACAGGACCTAGTTTTTTCTTATCCAGAATCAAAAACAAAAGCTCTCAATGGGCTTAATTTTACCATTCCAGATGGTAAATATGTTGCTATATTAGGTCACAATGGATCAGGTAAAAGTACATTCAGTAAATTGCTTGTTGCTTTGTATAAACCTTCTTCAGGTTCTATTGAAATAAATGGAACAGTTATTTCTAAAAAAACAATCAGAGAAATAAGAAAGAAAATTGGCATTATTTTCCAAAACCCTGATAACCAATTTGTTTGCGCTAGTGTTGAAGATGATATTGCCTTTGGTTTAGAAAATCAATGCATTCCACAAAAAGAAATGAAACCTATTATTGATGATTTAGCTGAAAAAGTAGGAATGAAGGACTATTTGACAAGAGAACCTCAATTCCTTTCAGGAGGTCAAAAGCAACGCGTTGCCATAGCTTCTGTTTTAGCTTTAAATCCTGAAATCATAATTTTTGATGAAGTAACTTCAATGCTTGATCCAAGAGGTAAAAGTCAAGTTTTAAAAATTATTAGAGAAATCCAAAAAACTCGTCAAAAAACTTTAATTTCAATTACTCATGATATGGATGAAGCTATTCAAGCTGATTATTGCTTAGTTTTTTCTAAAGGTCAAGTTGTTGCAAGTGGCTCACCTAAAGAAATTTTAAAAAATCATGAAATCATTGAATTAGCTAAAATTGATTCACCTTTTATTTATAAGCTCAGTGAAAAAATTGAAAATATTGAATCAACATATAATGAAGAGGAGTTATTAAATCAACTATGCAAATAA", "aa": "MIKVQDLVFSYPESKTKALNGLNFTIPDGKYVAILGHNGSGKSTFSKLLVALYKPSSGSIEINGTVISKKTIREIRKKIGIIFQNPDNQFVCASVEDDIAFGLENQCIPQKEMKPIIDDLAEKVGMKDYLTREPQFLSGGQKQRVAIASVLALNPEIIIFDEVTSMLDPRGKSQVLKIIREIQKTRQKTLISITHDMDEAIQADYCLVFSKGQVVASGSPKEILKNHEIIELAKIDSPFIYKLSEKIENIESTYNEEELLNQLCK", "aa_hexdigest": "6741eb0135fe2cd9fd9938cfa29ade33", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59835", "ncbi_nrp_id": "WP_013527030.1", "uniref100_id": "UniRef100_A0AB32XCI8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59835", "RefSeq:WP_013527030.1", "UniRef:UniRef100_A0AB32XCI8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCI8", "uniref90_id": "UniRef90_C4XFY7" }, "psc": { "uniref90_id": "UniRef90_C4XFY7", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [], "id": "EFPKPENHBP_1436", "locus": "GCA000186005_01423" }, { "type": "cds", "sequence": "contig_1", "start": 781008, "stop": 781172, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTCACGTGATGGAGACAATGCACCTATGGCTATTATTGAATTAGTTAAAGGCGACAAAGTTAAAACAAACAAAGCAGAAAAAACATCAAAAGACAAAGACACAAAAACTACAGCATCAAAAGCAACAAAAAGCAAAAAAGATGCTAAAGTAACTAAATAA", "aa": "MVSRDGDNAPMAIIELVKGDKVKTNKAEKTSKDKDTKTTASKATKSKKDAKVTK", "aa_hexdigest": "32932a419cbea1c214d45bd3906cd3f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5837.681300000006, "isoelectric_point": 9.874316215515137 }, "id": "EFPKPENHBP_1437", "locus": "GCA000186005_01424" }, { "type": "cds", "sequence": "contig_1", "start": 781481, "stop": 782494, "strand": "-", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "RefSeq:WP_013354840.1", "SO:0001217", "UniParc:UPI0001E32F35", "UniRef:UniRef100_C4XFY9", "UniRef:UniRef50_D5E4Y4", "UniRef:UniRef90_C4XFY9" ], "nt": "ATGGAAAAAATGACAAAATTAGAGTACCAAAAGGTTACTAAATTAAATAATTCAACTTCAAATGTAACTGCATTTACTCTTCAACCACTTGAACGTGGTATGGGTCAAACATTAGGTGTTGCTCTTCGTCGTATTTTACTTTCAAACATTACTTCATTAGCATTATTTGCAATCAAAATTGAAGATGTTAATCATGAATTCCAAGTAGTTGATGGTTGCGTAGAAGACGTTGCCACAATTATTATGAATTTAAGAAAAGTAAAATTCCAATACAACCCAGAATTTGTTAAAGATGACGAAATTATTAAAGTAACATTAAAAGCTGATCAACCAGGAGAAGTTACTTCAAGATTATTAGAAGTAAACAACTCAAGTATTGAAATTTTAAACAAATCATTAGAAATTGCTCACTTATCAGCAAATGGTAAACACAAATTAAATATTGAATTATATTTAAGACCAGGTCGTGGCTTTATTTCTAATGAAGAAAATAAGAAATTACTTAATAACTCTTCAATTACAACTAGAATGGAATCAAAAATCAAAAACGGAATTTTTATTGCTACAGATAGTAACTTTAGTCCAATTGAAAAAGTTAACTACACAGTTGAAGAATTAAACTCATCATCAAACAAAATTGAAGAAAGATTAACCTTCTCATTCCAAACAGATGGAACAGTTGATCCTAAGAGTGCATTACAACAAGCTTGTGAAATCTTAGTTGGTCACTTCAAATTGATTGGTAATGTTGATGAAATGAAAGTAAATGTATTTGCAGAAGAAAGTGAACAAACTCAAGAAACAAACGACAATGATGTTGATATCAATTCATTAAATCTTTCGGTACGTTCATTGAATGCACTTAAAAGAATTGGTAAAACAAAGATTTCTCAAGTAGCTGAAATGTCACTTGAAGAATTAGAACAAGTTAAAAACCTTGGTCGTAAATCACTTGATGAAATTCAACAACGTTTAAAAGAATATGGATATGAACTAAGTAAAGGAGAAGAATAA", "aa": "MEKMTKLEYQKVTKLNNSTSNVTAFTLQPLERGMGQTLGVALRRILLSNITSLALFAIKIEDVNHEFQVVDGCVEDVATIIMNLRKVKFQYNPEFVKDDEIIKVTLKADQPGEVTSRLLEVNNSSIEILNKSLEIAHLSANGKHKLNIELYLRPGRGFISNEENKKLLNNSSITTRMESKIKNGIFIATDSNFSPIEKVNYTVEELNSSSNKIEERLTFSFQTDGTVDPKSALQQACEILVGHFKLIGNVDEMKVNVFAEESEQTQETNDNDVDINSLNLSVRSLNALKRIGKTKISQVAEMSLEELEQVKNLGRKSLDEIQQRLKEYGYELSKGEE", "aa_hexdigest": "e4a20ddfac56c0d6702415765ca2ce3c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F35", "ncbi_nrp_id": "WP_013354840.1", "uniref100_id": "UniRef100_C4XFY9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F35", "RefSeq:WP_013354840.1", "UniRef:UniRef100_C4XFY9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY9", "uniref90_id": "UniRef90_C4XFY9" }, "psc": { "uniref90_id": "UniRef90_C4XFY9", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_D5E4Y4", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4Y4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4Y4" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [], "id": "EFPKPENHBP_1438", "locus": "GCA000186005_01425" }, { "type": "cds", "sequence": "contig_1", "start": 782786, "stop": 782899, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACCAAAAAGAAAAATATAACAAATGGTGTAGCGCACATTCACTCAACAAACCAAAATACTATTGTTACCTTTGCTGACGAAAAAGGAAACGTAATTGCATGA", "aa": "MARKTKKKNITNGVAHIHSTNQNTIVTFADEKGNVIA", "aa_hexdigest": "edc136720f859cda4a2c66c2182ccb8f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4051.5889000000006, "isoelectric_point": 10.171128654479979 }, "id": "EFPKPENHBP_1439", "locus": "GCA000186005_01426" }, { "type": "cds", "sequence": "contig_1", "start": 782922, "stop": 783293, "strand": "-", "frame": 2, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02952", "RefSeq:WP_013354842.1", "SO:0001217", "UniParc:UPI0001A8C14C", "UniRef:UniRef100_C4XFZ1", "UniRef:UniRef50_P38017", "UniRef:UniRef90_A5IYW1" ], "nt": "ATGGCTAGAATTTTAAATATTGAAATTCCTAACAATAAACGTGTTGTTATCTCATTAACATACATTTATGGTATAGGAAGAACTAGAGCACAAGAAATTTGTGCTAAAGCAAAAATTGATGAAAATCTTAGAGTTAAAGATTTATCAGAAGAACAATTATCAGCAATTCGTGAAGTAGCTAAAGAATACACAACTGAAGGTGATCTTCGTCGTGAAACCAGTTTAAACATCAAACGTTTAATGGAAATCAAATGCTACCGTGGTATTCGTCACAGAAAAGGTTTACCTGTTAGAGGTCAATGTACACAAAAGAATGCTCGTACACGTAAAGGTCCTCGTAAAACAGTTGCAGGTAAAAAATCAACTAAATAA", "aa": "MARILNIEIPNNKRVVISLTYIYGIGRTRAQEICAKAKIDENLRVKDLSEEQLSAIREVAKEYTTEGDLRRETSLNIKRLMEIKCYRGIRHRKGLPVRGQCTQKNARTRKGPRKTVAGKKSTK", "aa_hexdigest": "b9ae51f6d5f1f476d803c12f5915d571", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C14C", "ncbi_nrp_id": "WP_013354842.1", "uniref100_id": "UniRef100_C4XFZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14C", "RefSeq:WP_013354842.1", "UniRef:UniRef100_C4XFZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ1", "uniref90_id": "UniRef90_A5IYW1" }, "psc": { "uniref90_id": "UniRef90_A5IYW1", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "EFPKPENHBP_1440", "locus": "GCA000186005_01427" }, { "type": "cds", "sequence": "contig_1", "start": 783304, "stop": 783417, "strand": "-", "frame": 1, "gene": "rpmJ", "product": "50S ribosomal protein L36", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02919", "RefSeq:WP_013354843.1", "SO:0001217", "UniParc:UPI0001E32F37", "UniRef:UniRef100_A0AB32XCA5", "UniRef:UniRef50_P20278", "UniRef:UniRef90_A0A944MPI7" ], "nt": "ATGAAAGTTAGAGCTAGTGTAAAAAGAATGTGTAAAGATTGTCGTGTTATCAAACGTAAAGGAATTATTCGGGTAATTTGCTCACAACCAAAACACAAACAAAGACAAGGATAA", "aa": "MKVRASVKRMCKDCRVIKRKGIIRVICSQPKHKQRQG", "aa_hexdigest": "137a65f66db9c2840d9e402850c0e6b2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F37", "ncbi_nrp_id": "WP_013354843.1", "uniref100_id": "UniRef100_A0AB32XCA5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F37", "RefSeq:WP_013354843.1", "UniRef:UniRef100_A0AB32XCA5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCA5", "uniref90_id": "UniRef90_A0A944MPI7" }, "psc": { "uniref90_id": "UniRef90_A0A944MPI7", "gene": "rpmJ", "product": "50S ribosomal protein L36", "uniref50_id": "UniRef50_P20278", "kegg_orthology_id": "K02919", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20278", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20278" ], "product": "Large ribosomal subunit protein bL36" }, "genes": [ "rpmJ" ], "id": "EFPKPENHBP_1441", "locus": "GCA000186005_01428" }, { "type": "cds", "sequence": "contig_1", "start": 783435, "stop": 783650, "strand": "-", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "GO:0003743", "GO:0006413", "RefSeq:WP_013354844.1", "SO:0001217", "UniParc:UPI0001A8C14D", "UniRef:UniRef100_C4XFZ2", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_C4XFZ2" ], "nt": "ATGGCAAAAGACGCAATCAAAATGAAAGCTGTTGTCAAAGAAGCTTATTCAACAGATGAATATGATGTTGAACTTGAAAACGGCATGCTCATTAAAGCTCACATTTCAGGAAAAATGCGTGTAAATCACATTCGTATTTTGCCTGGAGATACTGTCGATGTTGAAATTAGTCCTTACAATTTATCTTTAGGACGAATAACATATCGTCACAAATAG", "aa": "MAKDAIKMKAVVKEAYSTDEYDVELENGMLIKAHISGKMRVNHIRILPGDTVDVEISPYNLSLGRITYRHK", "aa_hexdigest": "af11d2dbf559feacad294a6fa64b008c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C14D", "ncbi_nrp_id": "WP_013354844.1", "uniref100_id": "UniRef100_C4XFZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14D", "RefSeq:WP_013354844.1", "UniRef:UniRef100_C4XFZ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ2", "uniref90_id": "UniRef90_C4XFZ2" }, "psc": { "uniref90_id": "UniRef90_C4XFZ2", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "EFPKPENHBP_1442", "locus": "GCA000186005_01429" }, { "type": "cds", "sequence": "contig_1", "start": 783747, "stop": 784400, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_C4XFZ3" ], "nt": "ATGATAACTATTAAAAATGAACAAGAAATTATTAATATTACTCAAAGCTGCAAAATCCTGGCAGAAGTTAAACAAATAGTTTATGACTTTGTAAGACCAGGGGTTTCATTAAAAGAAGTGGATTCAGTCGCTTTTAATGAAATAGTAAAAAGAGGTGCACAACCAGCTTTTAAAGGCTTATATGGTTTTCCAGCAACCTGTTGCATCTCAATAAATGAAGAATTGATCCATGGAATTCCAAGTGATTACATTATTCAAGATGGCGACATAGTTAAAGTCGACATGGGATGTACTTACAAAGGAATGAACAGCGATAGTGCCTTCACTAAAGGTGTTGGTAATATCAATGAAAGTGATAAAAAAATAATTAGAGTGGCTCAAGAATCATTCTATGCAGGTTTTAATGCGATTAAACCTGGTGCAAGAGTGGGAGATGTTAGTCACGCTATTGGTCAAGTAATAAGACAAAATAATTTATATACACCAAGTCAATACTGTGGGCATGGAATTGGACGTGAAGTTCATGAAGACCCTTATGTATTTAATGATGGCCATGCTGGTAGTGGACCATTATTAAAAGATGGAATGGTAATTTGTATTGAGCCAATGATTTTACAACAAAATGCTAGAATCAAAGTTAAAAAAGATAAATGA", "aa": "MITIKNEQEIINITQSCKILAEVKQIVYDFVRPGVSLKEVDSVAFNEIVKRGAQPAFKGLYGFPATCCISINEELIHGIPSDYIIQDGDIVKVDMGCTYKGMNSDSAFTKGVGNINESDKKIIRVAQESFYAGFNAIKPGARVGDVSHAIGQVIRQNNLYTPSQYCGHGIGREVHEDPYVFNDGHAGSGPLLKDGMVICIEPMILQQNARIKVKKDK", "aa_hexdigest": "013c6f1a702865bb9e88b7aa9bb30814", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_C4XFZ3", "query_cov": 1.0, "subject_cov": 0.8645418326693227, "identity": 0.995, "score": 434.0, "evalue": 2.4e-153, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "EFPKPENHBP_1443", "locus": "GCA000186005_01430" }, { "type": "cds", "sequence": "contig_1", "start": 784428, "stop": 785090, "strand": "-", "frame": 2, "gene": null, "product": "Adenylate kinase", "db_xrefs": [ "RefSeq:WP_013354846.1", "SO:0001217", "UniParc:UPI0001E32F39", "UniRef:UniRef100_C4XFZ4", "UniRef:UniRef50_F9UK13", "UniRef:UniRef90_C4XFZ4" ], "nt": "ATGATAAGTAAAATTAAACAACATATAGTTTTTATGGGGCCTCCTGGTGTAGGAAAAGGTACAGTGGCTGCTATAATAGCTGAAAAATATGGACTAGTACATGTTTCAACTGGTAATATCTTTCGTGAAGAAATAGCTTCACAAAGTGAACTTGGTTTACAAGTTAAAAAGATAGTTGAAGCAGGACAATATGTACCAGACGACATTACAAATACTATTGTTAAAAATAAAATTCAAGCTTTAATCAAAGCTAAAAAGATTATTATGCTTGATGGTTATCCACGTACTATTGACCAAGTTAACTTTTTAGATAGTATTTCAAACTTTAAATATGTAGCTCTTGAATTAAGTGCTCCTGAAGATGTTATTTTAAAAAGATTAAATGGACGTAGACAATGTCCTAAATGTAAAGCAAGTTTCCATATTGATTTTATGCCTTCAACTAAAGGCAAATATTGTGATAAATGTGGAGCTGAATTAATCTTAAGAAAAGACGACTCAATTGAAGCTATTAAAGTACGTCAAAAAGTTTATCATGATCAAACAGCACCATTGCTTGATTATTACAAAAAATATAATTTATTATTAACATTTGATGCTACAAGTGATCCAGAAACTATAGCAAAAAATATTGTTGAATCTTTAGCGAAATTAAAAAACTAA", "aa": "MISKIKQHIVFMGPPGVGKGTVAAIIAEKYGLVHVSTGNIFREEIASQSELGLQVKKIVEAGQYVPDDITNTIVKNKIQALIKAKKIIMLDGYPRTIDQVNFLDSISNFKYVALELSAPEDVILKRLNGRRQCPKCKASFHIDFMPSTKGKYCDKCGAELILRKDDSIEAIKVRQKVYHDQTAPLLDYYKKYNLLLTFDATSDPETIAKNIVESLAKLKN", "aa_hexdigest": "d16dd8f9994701b2e5cea4a7b2b1b031", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F39", "ncbi_nrp_id": "WP_013354846.1", "uniref100_id": "UniRef100_C4XFZ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F39", "RefSeq:WP_013354846.1", "UniRef:UniRef100_C4XFZ4" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ4", "uniref90_id": "UniRef90_C4XFZ4" }, "psc": { "uniref90_id": "UniRef90_C4XFZ4", "product": "Adenylate kinase", "uniref50_id": "UniRef50_F9UK13" }, "pscc": { "uniref50_id": "UniRef50_F9UK13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK13" ], "product": "Adenylate kinase" }, "genes": [], "id": "EFPKPENHBP_1444", "locus": "GCA000186005_01431" }, { "type": "cds", "sequence": "contig_1", "start": 785087, "stop": 785311, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTTTAGTATCAGTTGCTCTTGAAACTATTCAACAATTCATGGCACGCCGTAAAAGTCAACATCTTGCAAAACAAAAACGTCTTTCAAAAGAAGCTGCAGATGTGCTTAACTTCCAAAACAAATATCAAGAAAATGATCCTTTTGCAACAAATGATCAAAAATCTGAAGACGTTTCAAAAGAATATGATAAATACAAAAATGGAGATGGCTTATTATGA", "aa": "MMILVSVALETIQQFMARRKSQHLAKQKRLSKEAADVLNFQNKYQENDPFATNDQKSEDVSKEYDKYKNGDGLL", "aa_hexdigest": "1c758825e9f37433c9856efbff4ba77e", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8595.625100000001, "isoelectric_point": 7.965729713439941 }, "id": "EFPKPENHBP_1445", "locus": "GCA000186005_01432" }, { "type": "cds", "sequence": "contig_1", "start": 785351, "stop": 785605, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTCACTCAACCACTAGGCTTGTCATTATTGATTCTAATTACATTCTTCTTTAGTTACATAATTGGATTGCAACAATCAAGAATTGACAAGATTTCAGAAGACTTTGCAAAGAACTCAACATTTATTCCTGGTATTAGACCTGGAGAAGAAACTGAAGATTATTTAATAGGTGTTGTTTTAAGACTTTGCACCTTCAGTGGTTTCTATTTAGTATTCTTAGCTTCATTCCAATACATAATGATTTTATGA", "aa": "MQFTQPLGLSLLILITFFFSYIIGLQQSRIDKISEDFAKNSTFIPGIRPGEETEDYLIGVVLRLCTFSGFYLVFLASFQYIMIL", "aa_hexdigest": "0bb7717a2da83d7017885cd2d4ccfd93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9640.237099999995, "isoelectric_point": 4.715101432800293 }, "id": "EFPKPENHBP_1446", "locus": "GCA000186005_01433" }, { "type": "cds", "sequence": "contig_1", "start": 785618, "stop": 786478, "strand": "-", "frame": 3, "gene": "secY", "product": "Preprotein translocase subunit SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "nt": "TTGCTTTATACATTATTGTTGTTATCAATCTATATTTTAATGACAACAATCAAAGCTCCTTTTGTTAAATTATCTGATGTAAATATTAATGATGACCCATTCTTAAATACATTAAACCTTGTTGGCGGTGGAGGTCTTAAAAACTTCTCACTTGCAGCACTTGGAATTAGTCCATTCATTAATGCCAGCTTGATAATGTCTCTTTTACAAACCAAACTTTTCCCTCCAATTCAAAAACTTAGTCAAAGTGGACCACAAGGTCGGAGAAAATTAAATATCATTACAAGAATTTTGACTTTGTTTATTGCATTCCCTCAAGCTATCTTGCTTACTAAATCACTTAGTTTAGGTGACAATCCATTTATTGGATTTACTCCAATTCAAAAACTCCCTCGAGAAGTTACAATCTACTTAATATTGCCTTTAATCTTAATTGGTGGATCATTGTTCTCATTATTCATTGCTGAACAAATTACAGATAAAGGTTTAGGAAATGGTACAAGCTTAATTATTTTTGTTGGTATTGCATTCCAATTACCAAGTCAATTTAAAGGAGCATTTAGCTTATTTGTTGGTAGTGAAAGTACAGCAGCAATTTTTGTTGGAGCATTAAAATTCTTAACTTACTTATTTGTTTACTTTGCATTGTTATTTATTATTGCTTTAGTTTACAATGCTGAAAGACATGTACCTATTCAACAAATTGGTGCTGGACGTAGTAGAAATATTAATGAAATGGGTAAATTGCCAATTAAATTAAATCCCGGTGGAATTATGCCAATAATCTTCGCCTCAATGCTAGTCAGCTTCCCAATTATGATATCTAGAATACTTCCAAAAGGTAATTCTGGCAAATTATGA", "aa": "MLYTLLLLSIYILMTTIKAPFVKLSDVNINDDPFLNTLNLVGGGGLKNFSLAALGISPFINASLIMSLLQTKLFPPIQKLSQSGPQGRRKLNIITRILTLFIAFPQAILLTKSLSLGDNPFIGFTPIQKLPREVTIYLILPLILIGGSLFSLFIAEQITDKGLGNGTSLIIFVGIAFQLPSQFKGAFSLFVGSESTAAIFVGALKFLTYLFVYFALLFIIALVYNAERHVPIQQIGAGRSRNINEMGKLPIKLNPGGIMPIIFASMLVSFPIMISRILPKGNSGKL", "aa_hexdigest": "8dd4c90137bfa54a14085e9a79ba8af0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CD17B9", "query_cov": 0.9090909090909091, "subject_cov": 0.8843537414965986, "identity": 0.604, "score": 291.0, "evalue": 2.97e-95, "valid": false, "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A3B0PVK7" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PVK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "product": "Preprotein translocase subunit SecY" }, "genes": [ "secY" ], "id": "EFPKPENHBP_1447", "locus": "GCA000186005_01434" }, { "type": "rRNA", "sequence": "contig_1", "start": 786897, "stop": 789779, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9856410256410256, "score": 2775.7, "evalue": 2e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TTTTAAATAAGGAAGAGTTTGTGGTGGATGCCTTGGGTCTGAAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGTGGAGCCGGAAATATGCTATGAAACGGGGATTTCCGAATGGGGAAACCTAACTAGGGTAATGCCTAGTTGCCTTGTTCTGAATTCATAGGAACTTGAGTGAGACACCTTGTGAACTGAAACATCTTAGTAGCAAGAGGAAGAGAAAATAAACAATGATTTCTTTAGTAGCGGCGAGCGAACGAGAAAGAGCCCAAACCCAGCAATGGGGGTTGTAGGACGATCTACATGAAGTTACAAAACTTAATGATAGCAGAAGCTTTTGGGAAGAAGCGGCATAGAGGGTGATACCCCCGTATGTGAAATTGTTAAGTCTTCTGATCGTATCCTGAGTAGGGCGGGGCACGTGGAACCCTGTCTGAATCTGCCGGGACCACCCGGTAAGGCTAAATACTAATCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGAGAGGGGAGTGAAATAGATTCTGAAACCACTTACTTACAATTAGTCAGAGCCCGTTTATGGGTGATGGCGTACATCTTGCAGTATGGACCGGCGAGTTATGACAGCATGCGAGGTTAAGCAGAAAAAAGCGGAGCCGTAGAGAAATCGAGTCTTAATAGGGCGTCTTAGTATGTTGTCATATACCCGAAACCATGTGATCTATTCATGAGCAGGCTGAAACTTGGCTAACCCCAAGCGGAGGGCCGAACCGTAGTACGCTGAAAAGTGCCCGGATGACTTGTGAATAGTGGAGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCCTCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAGTAGTGGTGGTAGAGCACTGAATATGGAATGGCCTCGCCTAGGGGTACTGACTATAATCAAACTCCGAATACCGCTATGTATTGCTATGCAGTCGGAACCGGGGTGCTAACGTCCCGGCTCGCGAGGGCAACAACCCAGATCGCCGGCTAAGGTCCCAAAATCGTGTTAAGTGAGAAAGGTTGTGAGGTTTCATAAACAACTAGGAAGTTGGCTCAGAAGCAGCCACCTTTTAAAGAGTGCGTAATAGCTCACTAGTCAAGAGACCTTGCGCCAACAATTTAACGGGACTAAAACACGATACCGAAGCCGCGGGTAATTATTTTACGTTAGAGGAGCGTTCCTAGGGCAATGAAGTCAGACCGTGAGGACTGGTGGAGCGCTAGGAAGTGAGAATGCCGGTATGAGTAACGATTCGTAGTGAGAATCTACGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGACCTAAGACGAGGCTGAAAAGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTATAATAGTGATGGAGTGACGGAGGAGGATAATCTTACCACTTACTGGATTGTGGGGTAAGCGTTCACTGGTGACTGTAGGCAAATCCGCAGTCTATAACCGGGAGGCGTGATGCATAGGGTCAAACCGAATTAGATGATTCCACACTTCCAAGAAAAGCTTCTAAACTTAATATTATGGAAACCTGTACCGAGAACGGACACACGTCCCCGAGATGAGTATTCTAAGGCGAGCGAGAAAACTAGTGTTAAGGAACTCTGCAAAATGACCCCGTAAGTTCGCAAGAAGGGGCGCCTAAGGTAACACTTAGGCCACAGTAAATTATGAGGGGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAACCGCAAGGTGATGTATAGGGGGTGAAGCCTGCCCAGTGCCCGAAGGTTAAGTGGATGCGTTAGCATTTGCGAAGCGTTGAAATGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGCGCAATGATCTCTCAACTGTCTCAACACTAGACTCGGTGAAATTATGGTCCCAGTGAAAACGCTGGGTACCCGCATCAAGACGAAAAGACCCCATGGAGCTTTACTACAGTTTCGTATTGGAACTTGGTCTAACATGTGTAGGATAGGTGGGAGACTTTGAAGCGAGGACGCTAGTTCTCGTGGAGTCATCCTTGAAATACCACCCTTGGTATATTGAGTTTCTAACATGCCATCATCATCAGGTGGGTGGACAGTGCGTGACGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGAGTAACGGAGGCGTTCAAAGGTACACTCAGTACGGTCAGAAACCGTATGTAGAGCGCAAAGGTAGAAGTGTGCTTGACTGTGAGACTTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGCTGACCAATACTAATAAATCGATCGGTTTAAAAGT", "id": "EFPKPENHBP_41", "locus": "GCA000186005_01435" }, { "type": "rRNA", "sequence": "contig_1", "start": 790093, "stop": 791605, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9869536855838226, "score": 1506.9, "evalue": 1.7e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGGCGTTAAATTTTGGGGCTCAACCCCAAAACGCGTTGGATACTGGCAGGCTAGAGTTGTGTAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAAGATGGCGAAGGCAGCTAACTGGACATATACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATCATTAGCTGATGGGGAACTCATCGGCGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTACGTTCGCAAGAATAAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATGGAGACATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCTGCAACGAGCGCAACCCTTATCCTTAGTTACTACCATTCAGTTGAGGACTCTAAGGAGACTGCCCGAGTAATCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCCGGTACAAAGAGAAGCGAAGTGGTGACATGGAGCAAACCTCAAAAAACCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTATAAACAAACTGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "EFPKPENHBP_39", "locus": "GCA000186005_01436" }, { "type": "cds", "sequence": "contig_1", "start": 791856, "stop": 792989, "strand": "-", "frame": 2, "gene": null, "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "RefSeq:WP_013354848.1", "SO:0001217", "UniParc:UPI0001E32F3B", "UniRef:UniRef100_A0AB32XCZ2", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_C4XDW7" ], "nt": "ATGTACAATAAGATTTTAATTAGATATGGTGAATTGACTCTAAAAGGCAAAAATAGAGTTAATTTTATTGATACTTTAGCTTCAAATATTCGTCATAAAACTAAAGATAAATTAGAGGTTGAATTTGACCGTATGTTTTTGCCTTATAGTCAAGAAAATTTAGATGCATTGCAATACATTTTTGGTATTACTTCTTACTCACCTGTTATTATTTGTGCTAATGATATTGAAGAAATTAAAAAGCAAGCTTTAAATTTAGCAAAAAATAAAAATGGAACTTTTAAAGTTGCTGCTCGTAGAAACAATAAAAGTTTTCAATACCAATCAAGTCAAATTAACAATATAGTTGGCGGTTTTTTATTAGAAAACAACCAAAATTTAAAAGTTGATGTTAATAATCCAGACTTTATTGTTTATATTGAAGTTAGAGGCAAATTTACTTATGTTTTTAGTGATTTTGTCCAAGCTTTAGGCGGCCTCCCTGTTGGTGTTAGTGGGCAAGTATTGCACTTAATGAGTGGTGGAATTGATAGTCCTGTTGCTGCTTTTCAACTTATGAAAAGAGGTCTTAAAGTTAATTTCTTATCTTTTATTACACCTCCTCAAACTGATCAAGCAACAGTAAATAAAATGAAAGAATTAATCAAAATATTAACTAAATATCAAAACACAAGCACTTTTTATTTAGCTAACTATTCTACTTTAATGAACTACATAGCTTTAGTTTCAAATCAAGCTTATAAAATTACTCTTATGCGTCGGAGCTTTTATCGTATTGCTGCTAAATTAGCTCAAAGTAAAAAAAATCTAGCCATTTCTAATGGCGAAAACTTAGGTCAAGTAGCTTCACAAACTTTAGAATCACTTTATACAATTAGTCATGATATTAACATGCCAATTTTGCGGCCACTATTAACTAATGACAAAATTGAAACAATCAATTTAGCTAAAAAAATTCAAACCTATGATCTTTCAATTACTAAATGTAATGAAACCTGTGAGCTTTTTGCTCCTAAAGAACCAGTAACTAAACCTAATGTTCAAGAAGCTCTTAAATTAGAAGAAGAATTAAACATGATTAATGACTTAGAAAAAGAATGCTTAGAAAAAAATATTGAAATAATCAAATTCTAA", "aa": "MYNKILIRYGELTLKGKNRVNFIDTLASNIRHKTKDKLEVEFDRMFLPYSQENLDALQYIFGITSYSPVIICANDIEEIKKQALNLAKNKNGTFKVAARRNNKSFQYQSSQINNIVGGFLLENNQNLKVDVNNPDFIVYIEVRGKFTYVFSDFVQALGGLPVGVSGQVLHLMSGGIDSPVAAFQLMKRGLKVNFLSFITPPQTDQATVNKMKELIKILTKYQNTSTFYLANYSTLMNYIALVSNQAYKITLMRRSFYRIAAKLAQSKKNLAISNGENLGQVASQTLESLYTISHDINMPILRPLLTNDKIETINLAKKIQTYDLSITKCNETCELFAPKEPVTKPNVQEALKLEEELNMINDLEKECLEKNIEIIKF", "aa_hexdigest": "eb42021110b1507bb89351709575da15", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32F3B", "ncbi_nrp_id": "WP_013354848.1", "uniref100_id": "UniRef100_A0AB32XCZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F3B", "RefSeq:WP_013354848.1", "UniRef:UniRef100_A0AB32XCZ2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCZ2", "uniref90_id": "UniRef90_C4XDW7" }, "psc": { "uniref90_id": "UniRef90_C4XDW7", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2" }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [], "id": "EFPKPENHBP_1448", "locus": "GCA000186005_01437" }, { "type": "cds", "sequence": "contig_1", "start": 792979, "stop": 793128, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGCATGCATTATCGATTTAATAATCCAGACTTTAATGAACTCTTAATTTCAGTCAATAAACACATGCGAGCTAAGAAATTTGATGAAGCTTTCTCACTACTAGCAACCTGCATGAAAAAAGGAAAAAAATATGTACAATAA", "aa": "MLKSMHYRFNNPDFNELLISVNKHMRAKKFDEAFSLLATCMKKGKKYVQ", "aa_hexdigest": "e844a480200caf064afe2d9ccdfffa01", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5852.8982000000005, "isoelectric_point": 9.844273948669432 }, "id": "EFPKPENHBP_1449", "locus": "GCA000186005_01438" }, { "type": "cds", "sequence": "contig_1", "start": 793357, "stop": 793761, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAAGAATCAAAAAAATCTATGCTGATTATTATACTTTAAACAAGAAAACTGCTCTTAATGCTGAGTTTCAAGATTTCATAAATAATGAATTAGAAAACATAGGCAAAATGATTAATGAGCTTGATCAAAAATTAAGCTATATTTCAATTGATTTAGATGATTTTGACAGCAATTTTGTAACCAAGATTTCACAAGAATTGTTACAACTTAGAAACCAATATGATAGCTTTAATAAAGCTAATAAAGCTAATGGCAATGTTAGTTTAATGGAGATGCAAACACTACTTCATGGCATAATGGAATTAATCAAAAAATGCAATGATAAAATTGAGATTTTTAACCTCGATACTTATCAAAAAAATTACAATGCTTACTACTTAAAAATTTTGGAAACTTGA", "aa": "MQERIKKIYADYYTLNKKTALNAEFQDFINNELENIGKMINELDQKLSYISIDLDDFDSNFVTKISQELLQLRNQYDSFNKANKANGNVSLMEMQTLLHGIMELIKKCNDKIEIFNLDTYQKNYNAYYLKILET", "aa_hexdigest": "9c40a421b6c921aefbe53378678d0e5e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15871.892799999992, "isoelectric_point": 5.021918296813964 }, "id": "EFPKPENHBP_1450", "locus": "GCA000186005_01439" }, { "type": "cds", "sequence": "contig_1", "start": 794155, "stop": 794580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAATTAAAATTATTGTACCAATAGTTATTTTGGCCTGTTTAGGTCTTTTGGGCATTTTTATTTTCCTTTATATTAGAAAAACTAATCTTAATAAATACATTAAATTTTTAGATGATTCAACTAAAAATATTAAAAATGATCTAACAGGACGTAACGCAACAATTCAACGTTTTATTACTTTATCAAATACTCAAGAAACATATAAAGCTGCTTTAAATCAATTAAAAAGTTTAGACAACAAATTAAAAAAAATAATTGTTGATTTAAATGATAAATATAATGCATTAAGAAAAGCGGCTAAAGCTTATAAACTTAAAGAAGCTAAAAAACTTTACTTAGAAATTTTGCCTAAATATGAAGAATGCATTTCAATGCATAAACAATTTGATGAAAAAACTAAGAACTTAAATAAACATTGA", "aa": "MQVIKIIVPIVILACLGLLGIFIFLYIRKTNLNKYIKFLDDSTKNIKNDLTGRNATIQRFITLSNTQETYKAALNQLKSLDNKLKKIIVDLNDKYNALRKAAKAYKLKEAKKLYLEILPKYEECISMHKQFDEKTKNLNKH", "aa_hexdigest": "7884f6c7642278e0466e9921c9a21e0a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16434.35519999999, "isoelectric_point": 9.778967475891111 }, "id": "EFPKPENHBP_1451", "locus": "GCA000186005_01440" }, { "type": "cds", "sequence": "contig_1", "start": 794684, "stop": 794884, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013354850.1", "SO:0001217", "UniParc:UPI0001E32F3D", "UniRef:UniRef100_A0AB32XC94", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_C4XDW9" ], "nt": "GTGGCTATAGTTCCAAAACGTAAGACATCTAAACAACGTAAACACAAAAGACGTACCCATGATGCTTTACCTGTTCAAAATCTTGTTGCATGCAAAAACTGTTCTCAATTAATTCAACAACACCGTGTATGTGAATCTTGTGGATTTTATAAAGGTAAAAAAGTTGAAGGTTACAAAAGTTTAGATATGCGTAATGCTTAA", "aa": "MAIVPKRKTSKQRKHKRRTHDALPVQNLVACKNCSQLIQQHRVCESCGFYKGKKVEGYKSLDMRNA", "aa_hexdigest": "ed8e2872a40e75b33585b60cb4069913", "start_type": "GTG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32F3D", "ncbi_nrp_id": "WP_013354850.1", "uniref100_id": "UniRef100_A0AB32XC94", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F3D", "RefSeq:WP_013354850.1", "UniRef:UniRef100_A0AB32XC94" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC94", "uniref90_id": "UniRef90_C4XDW9" }, "psc": { "uniref90_id": "UniRef90_C4XDW9", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "EFPKPENHBP_1452", "locus": "GCA000186005_01441" }, { "type": "cds", "sequence": "contig_1", "start": 795047, "stop": 795793, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATTAATGAACAACTAAAGAGTGCAAATTATCAAATTTATCAAAATGAAATGAAGCAAATTTTAGATGATTTTTATAGCAAAAATGGAGATTATAAAAATCAAAAAATTCAATTTACATTACCTATTTTTGCTGAAAAGAGTTCATTTATTGACAGTTTATATAAAAGAATAGCCTTCTTGTACAACAACCTAGACAATCGTTTAAATGTTAATTTTGAATATGCTTCAAAAGAAGCAAATAATTACTTTTCTAAATATCAAAACTTTAGTGCAGTTGATAATTCATTTAGTAACTATTTAATTAATTTAATAACAGCAAATAACCAATTCTTTTTAAATTTATTCTTATATTCAAAAGTCAATTTAGAATCAATTAACTATGATAAAAATATCAAAAAAATTATTTCAACAATAGAAGAAACAATTAATAAAGTTGATTACTTTAAAAATTATGAGTTGCAAAAAATTAAAGCAAACAGTTCACTTTTAGATCAAATTTTGCAAAAAAATAATTTGAAATTGAAAGAATTCAAAGCTAGATTTAAAGAAAAAATTCAAGCTTTAAATTTTGATCAACAATTCAATATTTTAAAAAGTATTGACTCATTTTTAGGAATCAGAGTAACTGAAGATAATTTCCTTTTTACTAACGAATACCAAAAATCAATAGTTCAATACTTCTACACAAAACCAATAAATCTAGATGGTTTTACTTATTTTCAAGATATTACTTTTAACTAA", "aa": "MDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN", "aa_hexdigest": "b6e3b16ac68c30c558a6730a4e6cb1bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29635.134500000044, "isoelectric_point": 8.384322929382325 }, "id": "EFPKPENHBP_1453", "locus": "GCA000186005_01442" }, { "type": "cds", "sequence": "contig_1", "start": 795998, "stop": 797077, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0", "UniRef:UniRef90_C4XDX0" ], "nt": "GTGACTCTGAAGGATAATAGTCAAAAAATTTTTAATAGTGATGATGTTAAAGAAAAAGTTGATCAAGCTCTTCAAAGTAGTACTAAAGTTAATGAAGATGGAGCATTAAGCGGTATTAAACAAATTTATTCTGATGAAAATAATAATATAAATTCTAAAGAATTTTTTGAAGCTTTAAATAGTGCAACTAAAATTGAATTTGAATTAAAAGACGATGTTTATTATTCAGATTATAAAGGCAAAAAAACAAATCATAAATTACAAGCCCAAGACTATTTAACTAGCTTTAATTTGAAACAAAATCGTGAACAATTTTCAGCTCTTTTAGACAACTATTCTTTGAAAAATTTAGGCCAAAATAGCATCCAAAATAACAAGTTAATTTTTGAATTAAACGATGCTCAAAAAAGCAAAAATAATAACTTTATTAAACTATTTACAACTCAAGAAATTACTAATAATTTGATCTTCAATCCAATATCAACAGACTACTATAAAAATAATAAAAATAGTTATGGCAAAAATTACCAAAACATCTGTTTTATAGGTTATTATGTTTTAAATAAGAACACTGTTGATGAGCAAATTTTTATTAAAAATAATAACTCTGCTTTAGATAGTTTTAACCAAAATGAAAAGCAAAATATTCTTAATAAAGTGACTATAAAATATAATCCAATTCAAAACGATGAGCCTACCTTTAGATTACAAAGTTATAATGCATTTAAACAAAATCTTTTAAGTGAAGCTTCTTATGATATTTTTAACCAATTGCAAAAAGATGAAATAGATAATTTCTCTAATCTTTTTGGTGTTACTTATACATTATTTAAACCAAATAATAGTAATGTTATAAGCTATTTTTACAATGTTAATCCATATGCTAAAAATAACAAAGATTTCAAATTTAATGAAGCTTTTAGCTTACTATTTTATAATCAGAAATTAAATGAATTAAAAGATCAAAATTTAGTAAATAAAAAATATTATGAACCACATCAAATTGTGTTCAGAAATATTTTAAATAACTGTATTAATCAATATTCATTTATTAAAAATTTGAATCATAATACTTACTGA", "aa": "MTLKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQNDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLNELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTY", "aa_hexdigest": "736004efb93a7cc0682ce9e6cea033de", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 795695, "stop": 797377, "observations": { "mutation": [ 796000, 797176 ], "stop": [ 796000, 797176 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX0", "query_cov": 1.0, "subject_cov": 0.45790816326530615, "identity": 0.997, "score": 688.0, "evalue": 3.88e-243, "gene_start": 1, "gene_end": 359, "reference_start": 109, "reference_end": 467, "reference_sequence": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNPKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIRWKSLGKSKFDHLNKFFYKTTTRFLEFYLAKSITVTLKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQNDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLNELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTYWNSFASQSLNYDSSNNSGILDNQNVNFLDDINRVYDYHFDYKTNKFDNKIIDFNGSDGLIKHSINSKNILDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN" }, "paralog": false, "identity": 0.9964285714285714, "score": 1123.0, "evalue": 0.0, "description": "Internal stop codon at 796000, 797176. Nonsense mutation around 796000, 797176.", "psc": { "uniref90_id": "UniRef90_C4XDX0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX0", "UniRef:UniRef50_C4XDX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0" ], "product": "Lipoprotein" } }, "id": "EFPKPENHBP_1454", "locus": "GCA000186005_01443" }, { "type": "cds", "sequence": "contig_1", "start": 797174, "stop": 797401, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAAATTAAAAAATATGGCAGCTTATTTTTAATTAGTGCAACACTTACTTCTAGTGTCGCTACTCTTTCATGCAACAATATTAGACAAGTTAATCCTAAAGATAGTTATATTAAAAAATATAATGAACCAAACAAAATTAAAGACAATTTTATTATTAACAAAGTTAAAAGAATTGATAATGATTTTGAGAGTTTATTAACTGCACCTTTAATTAGATGA", "aa": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNPKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIR", "aa_hexdigest": "a39414891b0d2d57b81b953746b23222", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8661.062799999996, "isoelectric_point": 10.00628299713135 }, "id": "EFPKPENHBP_1455", "locus": "GCA000186005_01444" }, { "type": "cds", "sequence": "contig_1", "start": 797410, "stop": 797619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTAGCTTCATTAGCTTTCTTTAATATTTATAAAATTGATCAAAGCTTAAATATTGGAAACGTTTTTAAAGATATAGTTGACAATCTAAAAAATGTTGAATATACAATTTTTGTTTCAATATTAGCAAGTTTTTATGTAATATTAGTAAAATTCTTAGGTATAAATTTCTTTATTGCGAGTGTACCTAAATTAGAAAAGTAA", "aa": "MLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILVKFLGINFFIASVPKLEK", "aa_hexdigest": "f37d752acdb7695fd3bab4d7348c5f6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7835.241599999996, "isoelectric_point": 8.006925010681151 }, "id": "EFPKPENHBP_1456", "locus": "GCA000186005_01445" }, { "type": "cds", "sequence": "contig_1", "start": 797698, "stop": 798558, "strand": "-", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1", "UniRef:UniRef90_C4XDX1" ], "nt": "ATGGCATTTAATCATGAGCACCACCATTCTCACGAAAAAGCAGGCCAAAGTCATATTGAATTTGAAAAAAGTCTTTTCACTTTAACTCACCATAAACACTTCCAAGTAAATATCAGCAATAACAAAAGTGAATTTAAACAATACTTAATTCGTTTCTTTAGCAAAAAGATCAACATTGCTTTAGTTTCAATTATTGGAATCTTCTTACTGTTTTTGACTTTAAGTGCTATTTTTAGCCCTTATTCGCCAAAAACTAGTGTCATCGATTCACAACTTGCTTATAATTTGCCTAACTACTTAAATGCAAGTATTACTAAAAAGTTTGCACAAAGTGACCCCTTATATCAATTTATTTTGCAAACTCAAAAAGATCATCCTAATTTAAAAATAGTCATAAATGAAACACCTGATTTTGACTTAGTTAATTTAACTTACAATCCATATCAATTGCTTTATGCCTTAAAAGGCACTAACTATATCTTTTTATATGGTACCAATTCTCAAGGTATAGACCGCTTTAGTTTCTTTATTCATTCATTTGGCATAAGTATTTTAGTAACATTTGTGGCTGCTTTAATCCAATTATTTTTAGGGACCTTTTTAGGTTCAATAGTGGCTTATTATTCAAATAAAAGCAGTGCTAAATTTAGTTACTATTTAATTAGTACTATTAATATTGTGCCTTTCCTTATTGTAGTTTTCTTGATTTTTAAAATAAGTTCATATAACCATCTTAATGCTATATTAATTTTAGGTTTTATTGGTTCCCTTAGCTTCTTTTATTCAAGTTATTCAATAGGGCTTGAAATCAAAAACAGTGAATTTATTTTAGCTTACAAAGCTAATGGCTTGAGTAATTGA", "aa": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSN", "aa_hexdigest": "b20b7d7adee4e54de4dedb54bcbec789", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 797410, "stop": 798558, "observations": { "mutation": [ 797661, 797700 ], "stop": [ 797661, 797700 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX1", "query_cov": 1.0, "subject_cov": 0.7486910994764397, "identity": 1.0, "score": 540.0, "evalue": 5.68e-192, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSNWRIIYKIIFIHCLWRNIALVSDSLSLNMLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILVKFLGINFFIASVPKLEK" }, "paralog": false, "identity": 0.9947643979057592, "score": 740.0, "evalue": 1.59e-273, "description": "Internal stop codon at 797661, 797700. Nonsense mutation around 797661, 797700.", "psc": { "uniref90_id": "UniRef90_C4XDX1", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX1", "UniRef:UniRef50_C4XDX1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "EFPKPENHBP_1457", "locus": "GCA000186005_01446" }, { "type": "cds", "sequence": "contig_1", "start": 798548, "stop": 799180, "strand": "-", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2", "UniRef:UniRef90_C4XDX2" ], "nt": "ATGACTGATTTAGTCCTCTCATTTATTGTCTTTATTTTAGCTGCTATTCCTACTTTTATCTTTGCACCAATAATGCTAATTATTTCAGAAGTCAATGACTTACCAGTTAATTTTATAGAGCCATCAGCTTTTGGTTTTGGTTATACATTGCTTTCTTTAATACTTCCTATTTCACTTTTATCTTTAGGAATCATTGCATTCTTTGCAACCATTACCAAAAATAATCTTGTTCAAATTCTAAAAAAAGATTATGTAATCACTTTAAAAGCAAATGGCTTAAGTACTTTACAAATATTTAATAAAGCTGTTATTAAAAATTTATTTATTTCATTAGTTAACCAAATAGTGCCAATTTTAGTTTTAACTATAAGTTTTAGCTTAATTATCGAACGTATTTTTCAAATACCAGGTCAAAGTGTTATTTTAATTAGCATGTTTGATCAAAAAGAAATTAATGTTATTATGGCTTTAGTATTTTTTAAAGCTTTGCTTTTATTCTTATTAGCTTTTATTTGTGAATTAACTTATGATTTATTGCAAGTTGAAAATGGTTATAATTTTAATTTCAATTTTAACTTCAAAGAAAATAGAGAAAAAGCTAGATTCAGAAAGGAGGCGCATAATGGCATTTAA", "aa": "MTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKARFRKEAHNGI", "aa_hexdigest": "f638ac15bf054f792df274ad072714b3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 798548, "stop": 799480, "observations": { "mutation": [ 799204 ], "stop": [ 799204 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX2", "query_cov": 1.0, "subject_cov": 0.6306306306306306, "identity": 0.99, "score": 350.0, "evalue": 3.49e-119, "gene_start": 1, "gene_end": 210, "reference_start": 124, "reference_end": 333, "reference_sequence": "MKRFFYVERWIFMNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGIWLGYKNNKMTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKDRFRKEAHNGI" }, "paralog": false, "identity": 0.9806451612903225, "score": 566.0, "evalue": 6.23e-207, "description": "Internal stop codon at 799204. Nonsense mutation around 799204.", "psc": { "uniref90_id": "UniRef90_C4XDX2", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX2", "UniRef:UniRef50_C4XDX2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "EFPKPENHBP_1458", "locus": "GCA000186005_01447" }, { "type": "cds", "sequence": "contig_1", "start": 799202, "stop": 799513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTAGCAAATACTTTTTCAAAAAAATTCTACTTACATTCTTAGGTATTTTTATAACAGTTACCCTTGGCTATTTTGTTATAGCTTTATTTATTAATAACTCACAAATAAACAGAGAACCAATTATCAAAAATTATTTCACTTTCTTAGGTTCTATTTTTAGTGGTTTTGGTAAACCATTTTCAACTAATAATAGTGGTTTCAATTCATCGTTAGAATTATTCTTCCATTATTATAAATTTAGTTTACTTTTTGTAAGCATTTCTTTTGTATTTAGCTTTTTTATAGGTGTTTTTGTCGGAATATGA", "aa": "MNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGI", "aa_hexdigest": "4782be8f2d41c46e4e81b958565d33d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11920.856699999988, "isoelectric_point": 9.756339073181149 }, "id": "EFPKPENHBP_1459", "locus": "GCA000186005_01448" }, { "type": "cds", "sequence": "contig_1", "start": 799567, "stop": 799836, "strand": "-", "frame": 1, "gene": null, "product": "Phosphocarrier protein HPr", "db_xrefs": [ "RefSeq:WP_013354854.1", "SO:0001217", "UniParc:UPI0001A8C336", "UniRef:UniRef100_C4XDX3", "UniRef:UniRef50_A0A1H9FWZ8", "UniRef:UniRef90_A0A3P8MEF8" ], "nt": "ATGAAAGAATTTACAGCTTCAATTATAGATCCAATTGGTCTTCACGCAAGACCTGCTAGTGTTGTTTCAACAATTGCTTCAAAATTCAAGAGTGATGTGCAAATTGTTTTAGACAAGACAGGAAAAGTTGGAAATCTTAAATCAATTATGAATGTTATGTCTTTAGGTGTTAAAAAAGGCGACGTAATTACTATTAAAATCAATGGAGCAGATGAAGAAGAAGCATACAAAGCTATTTATGATGCTTTTAAATCAAATGAAATTATTTAA", "aa": "MKEFTASIIDPIGLHARPASVVSTIASKFKSDVQIVLDKTGKVGNLKSIMNVMSLGVKKGDVITIKINGADEEEAYKAIYDAFKSNEII", "aa_hexdigest": "3b24c14dcfdb3c64b760de42af70285b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C336", "ncbi_nrp_id": "WP_013354854.1", "uniref100_id": "UniRef100_C4XDX3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C336", "RefSeq:WP_013354854.1", "UniRef:UniRef100_C4XDX3" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX3", "uniref90_id": "UniRef90_A0A3P8MEF8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEF8", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_A0A1H9FWZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A1H9FWZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1H9FWZ8" ], "product": "Phosphocarrier protein HPr" }, "genes": [], "id": "EFPKPENHBP_1460", "locus": "GCA000186005_01449" }, { "type": "cds", "sequence": "contig_1", "start": 800039, "stop": 800491, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "TTGATCTTAGATGAGCCTGCAGCAAACTTAGATCCAAAAGTTAGAAATGAACTTTATTTTAACTTAAGCAAACTTCACAAACAAGGCAAAACCATTTTTGTTTCTTCTCATATTTTAAGTGAGCTAGAACAATATATTGATAGTCTAACTGTTTTAGATAAAGGTAAAGTTTTATATTCTGGTGACTTAGAAATTTTAAAAAATTCAAACTTTGAAGTATTTATTAAAACTGAACAAATTAATGAAACTATTAAAGCCTTAGAAAAAGCAAAACTAGTTTCATCAAACAAAGAATATCAATGTCGAGAAACGGGTATTGCTGTTGATTTACCTTTCAATAAAATCACAAACATAACAAAAGCATTAATTAGTAGTAATATCGATTTTGAAAACATTGGAAAAAATATTCAAACCTTAAACGAAAAGTTTTTCAAAGTTGAAAAAGCAGAATAA", "aa": "MILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE", "aa_hexdigest": "6b69a40d31f2dd67ac384d48409265bd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 800039, "stop": 800791, "observations": { "mutation": [ 800575 ], "stop": [ 800575 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.49019607843137253, "identity": 0.993, "score": 288.0, "evalue": 3.69e-96, "gene_start": 1, "gene_end": 150, "reference_start": 157, "reference_end": 306, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.996, "score": 479.0, "evalue": 8.11e-174, "description": "Internal stop codon at 800575. Nonsense mutation around 800575.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_1461", "locus": "GCA000186005_01450" }, { "type": "cds", "sequence": "contig_1", "start": 800573, "stop": 800959, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "ATGTCACAAAACGCAATTGAATTTAAAAATGTTACAATGAGTTTTAAAAATTTTAAAGCTTTAGACAATGTGACATTTTCAATCAAAAAAGGTGAATTTCATGGATTTATTGGAGCAAATGGAGCAGGTAAAACTACAAGTTTCCGTTCATTATTGTATTTTTATCCAGAAGTCCAAGGTGAAATTTTAATAGATGGAATTAACTTTAAAAACAAAAAAAGTAGAATCAAAATTGGTTATGTTCCTGAAGTAGCAACATTTCCTAAAAGTCTGAATATTTATGATTACTTAGTTGCTTTAGCTGAATTAAATGGACTTAAAAATGACGAAGCTAAAAAAAGAATTGAAGAATTATTAATCGAGTTAGATGTTAACAAAAAAATATGA", "aa": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKI", "aa_hexdigest": "fefebced50cd257dea0e9a8c79248d32", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 800270, "stop": 800959, "observations": { "mutation": [ 800575 ], "stop": [ 800575 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.41830065359477125, "identity": 1.0, "score": 252.0, "evalue": 1.98e-82, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.9956331877729258, "score": 444.0, "evalue": 3.46e-160, "description": "Internal stop codon at 800575. Nonsense mutation around 800575.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_1462", "locus": "GCA000186005_01451" }, { "type": "cds", "sequence": "contig_1", "start": 801002, "stop": 801472, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCATTAATATATAGAAGTACCATTTTTCTCTCAGCTAATGAAGATCCTTCAAAAGCAAAAAATGAAGAAAGAGAATTCAGAAATTTTGTTGAAGATAATTTAACTTTTGACAAAAAAGAAGAATTATTTAAAAAAACTCGTTACTTAAGCTTTGACTATAACTCTATTGCAAAAAAACTTTTCTTTACAATCTTGGTAAAAAATAATTTATATTCTATAGTTGATGCAATGTCAAAAGTTATTATGAATGATTATCGCGAAACTTTAAAAATAAATCAAAATAAAGAATTCTTTAATTCAAGACTTGAAGAAATTTTTGGCAGCATGCTAATTAATAAAGATTTATTAAAAGAAAACAACATTGATTTGAAATTTTACAAAGTACAAAAAGTTAATATATTTCCATCTTACATGCAAATAATTATTTACTTTTTAATGGCTACACCTTTAGCTATTGGCGGATGA", "aa": "MVPLIYRSTIFLSANEDPSKAKNEEREFRNFVEDNLTFDKKEELFKKTRYLSFDYNSIAKKLFFTILVKNNLYSIVDAMSKVIMNDYRETLKINQNKEFFNSRLEEIFGSMLINKDLLKENNIDLKFYKVQKVNIFPSYMQIIIYFLMATPLAIGG", "aa_hexdigest": "9e6cfaac78431b44398c31123c76e476", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18518.32829999999, "isoelectric_point": 8.940878486633302 }, "id": "EFPKPENHBP_1463", "locus": "GCA000186005_01452" }, { "type": "cds", "sequence": "contig_1", "start": 801716, "stop": 801985, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTATAATGATTTTATTTTAGATGATCCAAAGAGCAACCGCTTTATTCCAATTATGAGTGAAGGAGTTCATAACCAATATTTCTATAAAAGAGATATGGAATCTGTTTTGAATAATTATGATTATGTCACAAATGGAAATCAAAAGATTTATATTATTGATTATGATTTAAGCACAACAGATCGACTTAATCCATATCAAAAACAAGAAACCTTAGAACAAAAAATTACTTATATGGCTCGCAAAAAACACATTATAAAATGA", "aa": "MNFYNDFILDDPKSNRFIPIMSEGVHNQYFYKRDMESVLNNYDYVTNGNQKIYIIDYDLSTTDRLNPYQKQETLEQKITYMARKKHIIK", "aa_hexdigest": "cc82759af5f94e1ac0d146dbe6ee141e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10841.131899999993, "isoelectric_point": 6.715038108825683 }, "id": "EFPKPENHBP_1464", "locus": "GCA000186005_01453" }, { "type": "cds", "sequence": "contig_1", "start": 802089, "stop": 802529, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTTCATTTTCTGCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAA", "aa": "MFISFSALLSKLAFKFSALLIFDIWWYSSGVEKILIMLKSAGVAINTGKVIPSNALDETFEKIIEINEITKVPIIRASKIKAICKSQILPINLVPFRSSDNPKAAKDIIIAENNALIVALITLIKNALTKEDQNSSVLDTGLEYKT", "aa_hexdigest": "d0431b10a5fc5bc7d7afb84b120e6534", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16096.851799999975, "isoelectric_point": 8.990196800231931 }, "id": "EFPKPENHBP_1465", "locus": "GCA000186005_01454" }, { "type": "cds", "sequence": "contig_1", "start": 802484, "stop": 802774, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATTATTAAAGTTAAACTTTAAAACAGCATACAAAGACAAGTCAATATTAATTATTAACTTTTTCCTAGTACCGTTTTTACTTTTAATAGGTACCCTTATTATTCTTATTGTATCTCACACAAATTCAGCTCGAGATATAGTTATTTATTATGGAAGCATGTCACCTATTTTAATTATATTGGCCGCAATTATTACGGGTTCTTATATTTTTAAGATGCCTCAAAATGAAGGCTTAACTTATGTTTTATATTCCAAACCAGTATCAAGAACTGAACTGTTTTGA", "aa": "MGKLLKLNFKTAYKDKSILIINFFLVPFLLLIGTLIILIVSHTNSARDIVIYYGSMSPILIILAAIITGSYIFKMPQNEGLTYVLYSKPVSRTELF", "aa_hexdigest": "14d51bf7c06e3b26995d4a4dfff6635b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10844.998199999989, "isoelectric_point": 9.646807289123537 }, "id": "EFPKPENHBP_1466", "locus": "GCA000186005_01455" }, { "type": "cds", "sequence": "contig_1", "start": 802911, "stop": 803177, "strand": "-", "frame": 2, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354857.1", "SO:0001217", "UniParc:UPI0001A8C339", "UniRef:UniRef100_C4XDX6", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_C4XDX6" ], "nt": "ATGATTACAAAAGAAGAAAAAGCTAAATTAGTTGCTAAGTATGGTGCTAATAAAAAAGACACAGGAAACACATTTGTTCAAATCGCAATTTTAACAGCAGAAATTGAAGATTTGAAAAAACACTTTGCAGCAAATCCTAAAGACAACCACTCACGTCGTGGTTTCTTAGCAAAAATTTCAAAACGTCGTGTTTTATTACAACACCTTAAGTCAAATGACTTAGAAACATACAACAAAGTTTTAGCTGAATTAAACTTAAGAAAATAG", "aa": "MITKEEKAKLVAKYGANKKDTGNTFVQIAILTAEIEDLKKHFAANPKDNHSRRGFLAKISKRRVLLQHLKSNDLETYNKVLAELNLRK", "aa_hexdigest": "ffd16c098c72fee1d0f9208017cda9d8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C339", "ncbi_nrp_id": "WP_013354857.1", "uniref100_id": "UniRef100_C4XDX6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C339", "RefSeq:WP_013354857.1", "UniRef:UniRef100_C4XDX6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX6", "uniref90_id": "UniRef90_C4XDX6" }, "psc": { "uniref90_id": "UniRef90_C4XDX6", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "EFPKPENHBP_1467", "locus": "GCA000186005_01456" }, { "type": "cds", "sequence": "contig_1", "start": 803499, "stop": 803918, "strand": "+", "frame": 3, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_013354858.1", "SO:0001217", "UniParc:UPI0001A8C33A", "UniRef:UniRef100_C4XDX7", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A5IZ35" ], "nt": "ATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAG", "aa": "MPTTNQLVTSGRVSKVRKQKAPALNLSFNTLTKEGRKLPAPFKRGVCTRVATMTPKKPNSAMRKYARVKLSNGMEVTAYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTQDLAGVNNRKQGRSLYGTKKEKAAK", "aa_hexdigest": "7688e7a0833844e3d3b9aa9f6605bfa5", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C33A", "ncbi_nrp_id": "WP_013354858.1", "uniref100_id": "UniRef100_C4XDX7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C33A", "RefSeq:WP_013354858.1", "UniRef:UniRef100_C4XDX7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX7", "uniref90_id": "UniRef90_A5IZ35" }, "psc": { "uniref90_id": "UniRef90_A5IZ35", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "EFPKPENHBP_1468", "locus": "GCA000186005_01457" }, { "type": "cds", "sequence": "contig_1", "start": 803944, "stop": 804252, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "nt": "ATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGA", "aa": "MSRKHSAPIREVLADPVFNSVLVTKLINTIMLDGKKSIAQDILYSAFNIIKEKTNKEPMEVFLAAVENVTPQLEVRTRRIGGTNYQVPTEVSDRRKQTLSLR", "aa_hexdigest": "a0f42759ce6eb781d4da3fa6591175b7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A2W4JVM3", "query_cov": 0.9901960784313726, "subject_cov": 0.8145161290322581, "identity": 0.505, "score": 111.0, "evalue": 1.39e-29, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A920EYB2", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920EYB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "EFPKPENHBP_1469", "locus": "GCA000186005_01458" }, { "type": "cds", "sequence": "contig_1", "start": 804256, "stop": 804411, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "GTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGA", "aa": "MQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANRAFAHFR", "aa_hexdigest": "338a9f9397997fad27c6d53d83c43be9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8431372549019608, "subject_cov": 0.86, "identity": 0.7909999999999999, "score": 73.9, "evalue": 1.41e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 1.5682771657248504e-18, "score": 63.072261810302734, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5905.67, "isoelectric_point": 9.983783531188966 }, "id": "EFPKPENHBP_1470", "locus": "GCA000186005_01459" }, { "type": "cds", "sequence": "contig_1", "start": 804426, "stop": 804581, "strand": "+", "frame": 3, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGGCAAGACAATATAAATTAGAAGATTACCTTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGA", "aa": "MARQYKLEDYLNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMD", "aa_hexdigest": "f4783571c42bba6996e71a462a482f55", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.706, "score": 80.5, "evalue": 3.84e-19, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 804426, "stop": 804646, "observations": { "deletion": [ 804630 ], "mutation": [ 804579 ], "stop": [ 804579 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.98, "score": 105.0, "evalue": 7.9e-29, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.9726027397260274, "score": 135.0, "evalue": 4.55e-43, "description": "Internal stop codon at 804579. Frameshift due to deletion around 804630. Nonsense mutation around 804579.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "EFPKPENHBP_1471", "locus": "GCA000186005_01460" }, { "type": "cds", "sequence": "contig_1", "start": 804834, "stop": 805949, "strand": "+", "frame": 3, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "nt": "ATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCGGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGA", "aa": "MDKAGADFFAAVKSVKERLNGNAVAIQIPIGAEAHFKGLVDLVEMKAYEYDGKPEENAKEIVIPSDLAELAKTKRQELIEAVASYDDDFMMQVLEGVEPDLATLKKVIRKATLTAEFYPAVCGTSFKNKGVKKMIDAVVDYLPSPLDIPATKAHLDDKEVAIPATDDHDFIGLAFKVMTDPYVGSLTFVRGYAGILNKGSYVYNSTKDVKERIGRLIQMHANSRQEIDECYAGDIVAAVGLKSTTTGDTLVAEKSPKYILEKMIFPEPVISQALEPESKDAMEKLALGLQKLAAEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRLKREHGVKAKVGAPQVSYRETITKSAEVEGKHIKQSGGKGQYGHV", "aa_hexdigest": "7bf6df4d164d87d183a46180eabbe2f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00255A9B97", "query_cov": 0.9892183288409704, "subject_cov": 1.0027322404371584, "identity": 0.504, "score": 356.0, "evalue": 1.78e-118, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A379SQ57" }, "pscc": { "uniref50_id": "UniRef50_A0A379SQ57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "product": "Elongation factor G" }, "genes": [], "id": "EFPKPENHBP_1472", "locus": "GCA000186005_01461" }, { "type": "cds", "sequence": "contig_1", "start": 806061, "stop": 806516, "strand": "+", "frame": 3, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "nt": "ATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAA", "aa": "MAGGILAGYPMIDIKATLFDGSYHDVDSSELAYKIAASKALTKAKDLLGTVLLEPIMDVAVVIPEDYFGDVMGDLTRRRGQIEDNETRNDGAHIIRSLVPLKEMFGYATDLRSMTAGRGTYQMQFHHYEKCPKNIADEIIKRRNIQVKDED", "aa_hexdigest": "7c133eebb2053ee313796d038b4a2d0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PQ45", "query_cov": 1.0, "subject_cov": 0.8628571428571429, "identity": 0.7809999999999999, "score": 246.0, "evalue": 1.33e-81, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A3B0PQ45" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "EFPKPENHBP_1473", "locus": "GCA000186005_01462" }, { "type": "cds", "sequence": "contig_1", "start": 807024, "stop": 807161, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1", "UniRef:UniRef90_C4XDY1" ], "nt": "ATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGA", "aa": "MYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIA", "aa_hexdigest": "d889d697825ad7dea3f2ec514fd6c993", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 806925, "stop": 807260, "observations": { "mutation": [ 806976, 807159, 807171, 807216 ], "stop": [ 806976, 807159, 807171, 807216 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY1", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 1.0, "score": 82.0, "evalue": 3.33e-19, "gene_start": 1, "gene_end": 45, "reference_start": 34, "reference_end": 78, "reference_sequence": "MKLVLEEYILTGSILSNWDKIQHSNVDRIGMLNMYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIAWKDGWPSLINNNSNALVQIWFDYYINNIMNLDE" }, "paralog": false, "identity": 0.963963963963964, "score": 203.0, "evalue": 1.92e-69, "description": "Internal stop codon at 806976, 807159, 807171, 807216. Nonsense mutation around 806976, 807159, 807171, 807216.", "psc": { "uniref90_id": "UniRef90_C4XDY1", "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY1", "UniRef:UniRef50_C4XDY1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5066.796700000002, "isoelectric_point": 8.064301872253417 }, "id": "EFPKPENHBP_1474", "locus": "GCA000186005_01463" }, { "type": "cds", "sequence": "contig_1", "start": 807411, "stop": 807647, "strand": "+", "frame": 3, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707", "UniRef:UniRef90_UPI000F03D707" ], "nt": "TTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGA", "aa": "MNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFN", "aa_hexdigest": "7361005b9c6b6c11cf413be80a4df78c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 807384, "stop": 807887, "observations": { "mutation": [ 807645, 807858 ], "stop": [ 807645, 807858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F03D707", "query_cov": 1.0, "subject_cov": 0.46706586826347307, "identity": 0.987, "score": 154.0, "evalue": 1.25e-46, "gene_start": 1, "gene_end": 78, "reference_start": 10, "reference_end": 87, "reference_sequence": "MDTRTIDDYLNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFNWNILHYRDKWSNEVDCILQLKNGDFAAIKIKLSNSYEEQTATGLLRFKKETEEEQKLAKINREKMYKKVPKWLIIISGTA" }, "paralog": false, "identity": 0.9760479041916168, "score": 318.0, "evalue": 1.05e-113, "description": "Internal stop codon at 807645, 807858. Nonsense mutation around 807645, 807858.", "psc": { "uniref90_id": "UniRef90_UPI000F03D707", "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F03D707", "UniRef:UniRef50_UPI000F03D707" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707" ], "product": "DUF4143 domain-containing protein" } }, "id": "EFPKPENHBP_1475", "locus": "GCA000186005_01464" }, { "type": "cds", "sequence": "contig_1", "start": 807696, "stop": 807860, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACTTAAAAATGGAGACTTTGCAGCCATTAAAATTAAATTAAGTAATAGTTATGAAGAGCAAACAGCTACAGGCTTACTTAGGTTTAAAAAAGAAATGGAAGAAGAACAAAAATTAGCTAAAATCAATAGGGAAAAAATGTACAAAAAAGTACCTAAATGA", "aa": "MQLKNGDFAAIKIKLSNSYEEQTATGLLRFKKEMEEEQKLAKINREKMYKKVPK", "aa_hexdigest": "8bd64d598b7c0ce0390fdad9750648b8", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6376.428400000001, "isoelectric_point": 9.646807289123537 }, "id": "EFPKPENHBP_1476", "locus": "GCA000186005_01465" }, { "type": "cds", "sequence": "contig_1", "start": 807993, "stop": 808364, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3", "UniRef:UniRef90_C4XDY3" ], "nt": "ATGGCTTTTATCTTTTATTTGTGCATCTTTATTATGCCAATTTACAAAGGTTTAGAGTATAAATATGACAATAATATAATGAAGCAAACTAAAGATTTAGCGGCGACTGCAGCAGCATTTTGTATGATAGTTGTAATTGTTTATTTAAGTATTACAGTTGAATCATTTCTAATCTTGAAAAAGTTAATAAAATTCCCTGAAGTAACAGCTGAAATTTATTATATGACTTTTCTTTTTCATAATGATCGTACAATGTTCAAAAATTATTTTGAAAGTGAAAAAATTAATCTAGATCCAAGAATTAAAAAAGAAATTATGGACATAGTTTCTAATGAATCAAAAGCCAAAGGAATAAAGATCATTTTTAGATAA", "aa": "MAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKNYFESEKINLDPRIKKEIMDIVSNESKAKGIKIIFR", "aa_hexdigest": "7c54bb72b7a9c82fba4767c6508ee47e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 807993, "stop": 808664, "observations": { "mutation": [ 808421, 808496, 808607 ], "stop": [ 808421, 808496, 808607 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY3", "query_cov": 1.0, "subject_cov": 0.5394736842105263, "identity": 1.0, "score": 237.0, "evalue": 1.11e-77, "gene_start": 1, "gene_end": 123, "reference_start": 106, "reference_end": 228, "reference_sequence": "MLETFNQEAQKYHTEEVKLRRDLSWSSLFAFISLLVAFTITVTFLSLALKNYDYYLSGLNYWYNFFVYIATSSIISLITLILACYFWIKFDRKGVSKYNIIASCLMAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKNYFESEKINLDPRIKKEIMDIVSNESKAKGIKIIFR" }, "paralog": false, "identity": 0.9865470852017937, "score": 427.0, "evalue": 4.26e-155, "description": "Internal stop codon at 808421, 808496, 808607. Nonsense mutation around 808421, 808496, 808607.", "psc": { "uniref90_id": "UniRef90_C4XDY3", "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY3", "UniRef:UniRef50_C4XDY3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14554.295399999986, "isoelectric_point": 9.001543235778808 }, "id": "EFPKPENHBP_1477", "locus": "GCA000186005_01466" }, { "type": "cds", "sequence": "contig_1", "start": 808948, "stop": 810042, "strand": "+", "frame": 1, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "EC:2.5.1.6", "GO:0004478", "GO:0005524", "GO:0006556", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P72871", "UniRef:UniRef90_C4XDY4" ], "nt": "ATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGACCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGA", "aa": "MNLNKQILFTSESVGQGHPDKICDQISDAILDEYLRKDPYSRCAIETMATGNMLIIAGEASSKANVDHKQIAVNVLKKAGQYSDHLRFHLDVKKQSTDISQGVIRANEEIGAGDQGIMFGYATNETRNYMPLAITLAHEIVKRADKLRERGLFKYAKSDMKSQVTLDYTDSKRIKVDTILFSCQHEENFNEKEFKEYIVKNIFEPVLQDYSLKLPVKILINPTGRFVIGGPIGDTGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSAAYACRYVTKNLVAAGCADKIELQVSYAIGVAEPVSILVETFGTEKIAKDKIVSIVHKLFDLRPKHIIESLNLRRPIYQQTSYFGHFGREDLDLP", "aa_hexdigest": "13f77e9da3324e0f50c0b51c052ed3c0", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_C4XDY4", "query_cov": 1.0, "subject_cov": 0.9405684754521964, "identity": 1.0, "score": 715.0, "evalue": 5.39e-260, "valid": true, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P72871", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_P72871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P72871" ], "product": "S-adenosylmethionine synthase" }, "genes": [ "metK" ], "id": "EFPKPENHBP_1478", "locus": "GCA000186005_01467" }, { "type": "cds", "sequence": "contig_1", "start": 810166, "stop": 810282, "strand": "+", "frame": 1, "gene": null, "product": "MarR-family transcription repressor", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5", "UniRef:UniRef50_C4XDY5", "UniRef:UniRef90_C4XDY5" ], "nt": "ATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAA", "aa": "MRKIVRAKNKERAAKRLHKLQLEEARDKRRAAREAEAK", "aa_hexdigest": "aa1de1c111f157ffb37add61c45210a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C342", "uniref100_id": "UniRef100_C4XDY5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY5", "uniref90_id": "UniRef90_C4XDY5" }, "psc": { "uniref90_id": "UniRef90_C4XDY5", "product": "MarR-family transcription repressor", "uniref50_id": "UniRef50_C4XDY5" }, "pscc": { "uniref50_id": "UniRef50_C4XDY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY5" ], "product": "MarR-family transcription repressor" }, "genes": [], "id": "EFPKPENHBP_1479", "locus": "GCA000186005_01468" }, { "type": "cds", "sequence": "contig_1", "start": 810380, "stop": 810559, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5983D", "UniRef:UniRef100_A0AB32XCG2", "UniRef:UniRef50_A0AB32XCG2", "UniRef:UniRef90_A0AB32XCG2" ], "nt": "ATGGTTCTCACTTTTATAACTTTTAGAATTGTGGTTCAAAATATAATACCTATTGATTTACTTGGAAATCTGAATATTGAAATTCAGGTACAAGATATTAAAACTAATGAAACATCTAAAGTAAGAAAATTTAATTTACTTTTTAAAAGTTTGCTAAATAAAAATAAAAGAGCCTTATAA", "aa": "MVLTFITFRIVVQNIIPIDLLGNLNIEIQVQDIKTNETSKVRKFNLLFKSLLNKNKRAL", "aa_hexdigest": "e5535fab7a1ca19bd4e6fb98eb2a65cd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5983D", "uniref100_id": "UniRef100_A0AB32XCG2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5983D", "UniRef:UniRef100_A0AB32XCG2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCG2", "uniref90_id": "UniRef90_A0AB32XCG2" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCG2", "uniref50_id": "UniRef50_A0AB32XCG2" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCG2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6870.1548, "isoelectric_point": 10.282916831970212 }, "id": "EFPKPENHBP_1480", "locus": "GCA000186005_01469" }, { "type": "cds", "sequence": "contig_1", "start": 810592, "stop": 810963, "strand": "-", "frame": 1, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354867.1", "SO:0001217", "UniParc:UPI0001A8C361", "UniRef:UniRef100_C4XDY6", "UniRef:UniRef50_Q5ZZS0", "UniRef:UniRef90_A0A3P8K8J4" ], "nt": "ATGGCAAAATTAACAAAAGAATCATTCATTTCATCATTAAAAGAAATGTCAATTAAAGAAGTTATGGAATTAGTTGAAGCAATGAAAGAAGAATTTGGAATTGACCCATCAGCTGTTGCTGTTGCTGCTGCTCCTGCTGCAGCTGCAGCTGCTGAAGGTCCTTCAGTAGTTACAGTTTCAATTACTTCAGACAATGGTAAAAAATTACCTATTGTTAAAGCAGTTAAAGAATTATTAAACTTAGCTTTAATGGATGCAAACAAATTAGTTTCAAACCTTCCAGCTGTTGTTAAAGAAAACATCAAACCAGATGAAGCAGAAGCTATCAAAGCTAAACTTGTTGAAGCTGGTGCTACAGTTGAAGTTAAATAA", "aa": "MAKLTKESFISSLKEMSIKEVMELVEAMKEEFGIDPSAVAVAAAPAAAAAAEGPSVVTVSITSDNGKKLPIVKAVKELLNLALMDANKLVSNLPAVVKENIKPDEAEAIKAKLVEAGATVEVK", "aa_hexdigest": "ef67351208754f1f940e309ae7ffc49c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C361", "ncbi_nrp_id": "WP_013354867.1", "uniref100_id": "UniRef100_C4XDY6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C361", "RefSeq:WP_013354867.1", "UniRef:UniRef100_C4XDY6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY6", "uniref90_id": "UniRef90_A0A3P8K8J4" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8J4", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_Q5ZZS0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZS0" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "EFPKPENHBP_1481", "locus": "GCA000186005_01470" }, { "type": "cds", "sequence": "contig_1", "start": 810985, "stop": 811494, "strand": "-", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013527046.1", "SO:0001217", "UniParc:UPI0001F59855", "UniRef:UniRef100_C4XDY7", "UniRef:UniRef50_Q98QS1", "UniRef:UniRef90_A0A3P8MDE2" ], "nt": "ATGCAAAAAAGACATGAATCTGCTTTTAGATTAGTTAAAAAAGATACAGTTAAAGAAATTCAAAATAAACTTGAATCTTCACAAGCTCTTGTTATAGCTGAATATCGTGGATTAAGTGTTGCTGAATTGACTTCATTACGTCAAGAAGCTAAAAAAGAAGGTATTGACATTAAAGTTTACAAAAACCGTCTTTTCAAAATAGCTGCTAAAAATCATGGCATTAATGATTTAGATCAACACTTAGTAGGACCAAACATTTTTGCTTTTGGTACAACCGATGCTCTAATTGCTGCAAAAGTTTTAGTTAAATTCTCTAAAGAAAACAAACTTTTGGTTCCTAAAGCTGGTATTTTTGAAGGCAAAGTTATCGATGCTAAAGGTGTTCAAGAAGTTGCTTCATTACCAAACTATGAAGAAGCTCTTACAATTCTTGCTCGTTCACTTATGTCACCATTACAACAACTCTCTCTTTCTCTAAAAATGTTTAGTGAAAAAGAAGAAAACAAATAA", "aa": "MQKRHESAFRLVKKDTVKEIQNKLESSQALVIAEYRGLSVAELTSLRQEAKKEGIDIKVYKNRLFKIAAKNHGINDLDQHLVGPNIFAFGTTDALIAAKVLVKFSKENKLLVPKAGIFEGKVIDAKGVQEVASLPNYEEALTILARSLMSPLQQLSLSLKMFSEKEENK", "aa_hexdigest": "93bac3e43720b579ced83327b01eaf7c", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59855", "ncbi_nrp_id": "WP_013527046.1", "uniref100_id": "UniRef100_C4XDY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59855", "RefSeq:WP_013527046.1", "UniRef:UniRef100_C4XDY7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY7", "uniref90_id": "UniRef90_A0A3P8MDE2" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDE2", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_Q98QS1", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QS1" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "EFPKPENHBP_1482", "locus": "GCA000186005_01471" }, { "type": "cds", "sequence": "contig_1", "start": 811901, "stop": 812452, "strand": "-", "frame": 3, "gene": null, "product": "Hemolysin C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8", "UniRef:UniRef90_C4XDY8" ], "nt": "ATGGCTCAAAATGCTTTAGACTTAGATAGTACCAAAGTTAAAAAACACTACATAAGATTAAAAGATGTATCTATAATTGATGCTAATGCTAATATTCAAGAAGCATTTGAAATTTTTAAAGATACAAACTATAGCCGTATTCCTGTTAAAAAAGATGAAAATTTAATTGGCATTTTACACTTAAAAGATGTCTTCTTTTTACAAAAAGGTAAAGTTATAAACTATTTTAAAACTGTGCCTACTATTAGTGCTAATGCTTCTCTTTCTTCGGCTCTAGAAAAAATGAGGTACCAAAGAGCTCAAATGGCTTTTGTTACACCAAATAACAACTCAAGTGAAATAATTGGAATTATTACAATTGAAGACATTATTGAAGAAATTGTTGGCGAAATTTATGATGAATTTGATGATGAAGAATTAAAAGACATTTTTGAAATTAGTCTTGAACACTTCCATGTCTCAGCTTCTTTAACAATGAAAGAATTAATTAAAAAACTAGAAATAGAATTAGATCTTGAAGAAGAAGAAAATGAATTAACACTTCATAAATGA", "aa": "MAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHK", "aa_hexdigest": "89d118c33660164ee6c9df534fe2acf8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 811718, "stop": 812752, "observations": { "mutation": [ 811903, 812578 ], "stop": [ 811903, 812578 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY8", "query_cov": 1.0, "subject_cov": 0.4255813953488372, "identity": 1.0, "score": 346.0, "evalue": 1.33e-116, "gene_start": 1, "gene_end": 183, "reference_start": 187, "reference_end": 369, "reference_sequence": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTSWLLSTYLGKKFESYNVLISTAVMTPIIVLFGEITPKLIAKSKPEATVKVFCYLLIILYYLFWPITYPISKIGKKIYITNTENDVKNIIDIAQNEGVLEANESIMAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHKWLEKRLGHKLYKNTKYEYNDVYFKVIDLGTNRFKNVRVDIELGNKADILNTDETLTDNEKN" }, "paralog": false, "identity": 0.9941860465116279, "score": 664.0, "evalue": 2.34e-244, "description": "Internal stop codon at 811903, 812578. Nonsense mutation around 811903, 812578.", "psc": { "uniref90_id": "UniRef90_C4XDY8", "product": "Hemolysin C", "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY8", "UniRef:UniRef50_C4XDY8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8" ], "product": "Hemolysin C" } }, "id": "EFPKPENHBP_1483", "locus": "GCA000186005_01472" }, { "type": "cds", "sequence": "contig_1", "start": 812759, "stop": 813010, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGACCCGAAACTGAAATTAGGATTAAGCATATTCGGACTAGTTATTTTGTTAATAGCTAGCGGAATTTTTAGTGCCAGTGAAACTGCTTATACATCCTTAAATGTTGGCAAAATTGAAACAATGGTTGGCAATAAAGAATTTGGCCACAAAATAATCAAAAAACAACATACATTTTTCAACCAAACTTTAAGTACTATTTTAATATGCAACAATATAGTTAATATTGCTGCTTCGGCCTTAACATCCTGA", "aa": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTS", "aa_hexdigest": "4539d5b404e9c43d6236a0c757912a45", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 69, "aa_cov": 0.8313253012048193, "hmm_cov": 1.0, "evalue": 1.9512999877045402e-18, "score": 63.04677200317383, "start": 14, "stop": 82 } ], "seq_stats": { "molecular_weight": 8940.45019999999, "isoelectric_point": 9.302997398376462 }, "id": "EFPKPENHBP_1484", "locus": "GCA000186005_01473" }, { "type": "cds", "sequence": "contig_1", "start": 813097, "stop": 813447, "strand": "-", "frame": 1, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "nt": "TTGATTTCATCAGACTCAAATAGAAGAAATGAAAGTCGGCAAAATGAAATAGCTAAAATTAGCCGTTCATTAAAAACATTAGCCTTAGAATTAAATGTTCCAATTATTGCACTAAGCCAACTTTCCCGTGAAGTTGAAAAACGTGAAGACAAAAGACCTATTATGTCTGACTTACGTGAATCAGGTAACATTGAACAAGATGCCGATATTGTTATGTTCTTATATCGTGAAAATTATTATAAAAAACAAGTCTCACAAGAAACCAAAGATCTCTTTGGAGATATTGGAGAAAAAATAGATATCATTATTGCAAAACACAGAAATGGACCAACAGGAATTTTTCCATTATGA", "aa": "MISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPL", "aa_hexdigest": "454095d752080a09bbd74ecb7cd29104", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001AC6C2CE", "query_cov": 0.9913793103448276, "subject_cov": 0.8455882352941176, "identity": 0.565, "score": 111.0, "evalue": 3.35e-29, "valid": false, "product": "DnaB-like helicase C-terminal domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A7W1AXE1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A7W1AXE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "EFPKPENHBP_1485", "locus": "GCA000186005_01474" }, { "type": "cds", "sequence": "contig_1", "start": 813508, "stop": 814437, "strand": "-", "frame": 1, "gene": "dnaB", "product": "replicative DNA helicase", "db_xrefs": [ "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260", "SO:0001217", "UniRef:UniRef50_C4XDY9", "UniRef:UniRef90_C4XDY9" ], "nt": "ATGCCAAATAATAATCCAAGTAATGCACCAATAAATACATCTCGTCATATTAACTCTAGATATGAGGAGTCTCTTTTAGGCTTAATCTTAACAGACAACAAAAACGCACCTAAAATTGTGCCTTACTTACTTGAAGAAGACTTTGCAATTTTAGAAAATCAAAGACTTTTCTCTGTTTTTAAAGAACTATTTGAAAACAACATTTCGATTAATGAAGAAAACATTTTTACTGTTGCTAAAAAAAGAAACTTTGTTCAAATAACACCTTTATATTTATCTAAACTTTATCATACTGCTGGTTTCTCATCTAATATTCAAACTTACTTAGAAGAATTAGTTAAGTTAACAAAATTACGTTTAATTGAATCACGTTTAGCTTTAGTTCAAAGAAGACTTGATGTTGAAAAAGCAATCAATGAAAAAGATGTTATTTCAGATTTACAAAACCTATTACTTGACATTGATCGTAGTCAAGTTAGTGCAGACTTTTTAACAGCCAAAGAAGTTAGCGATGAATACTTTAAAGATTTAGAAAATCGCCACTCAAGAGATCTAAATGAATTAAATGGACTAGCAACAGGTTACGACAATTTAGACCAAATTACTCAAGGTCTACATGGTGGTGAATTAATTATTATTGCTGCTCGTCCAGCAATGGGTAAAACTGCTTTTGCTCTTAATATTGCTTCAAATGTTGCTAAAAGAAAAAACAGAAGAGCTGTCTTCTTTACGCTTGAAATGTCATCAAACCAACTTATGGGTAGAATTTATAGCTTGAACACAAATGTGCCACTTCAAAAACTAAAACAACCTCAAACAATTACAGATAATGAATTTCTTTTAATCAACAGTTTAAAACAAGATGTAATTGACAAAATGAACTTATTTATAGATGAATCAGTAAATAATGAATTAAATACACTGCTTTGA", "aa": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFSVFKELFENNISINEENIFTVAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLL", "aa_hexdigest": "b6a30272d6c6d868258c3cee7841b824", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 813205, "stop": 814437, "observations": { "mutation": [ 813510 ], "stop": [ 813510 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY9", "query_cov": 1.0, "subject_cov": 0.6518987341772152, "identity": 1.0, "score": 586.0, "evalue": 2.12e-208, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFSVFKELFENNISINEENIFTVAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLLWKCRRLHKVSPIDLIVIDYLQLISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPLWFKMSCGRFINFENEQIAVSSDDDEEEE" }, "paralog": false, "identity": 0.9975609756097561, "score": 786.0, "evalue": 9.37e-290, "description": "Internal stop codon at 813510. Nonsense mutation around 813510.", "psc": { "uniref90_id": "UniRef90_C4XDY9", "gene": "dnaB", "product": "replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDY9", "go_ids": [ "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY9", "EC:5.6.2.3", "UniRef:UniRef50_C4XDY9", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY9" ], "product": "Replicative DNA helicase" } }, "id": "EFPKPENHBP_1486", "locus": "GCA000186005_01475" }, { "type": "cds", "sequence": "contig_1", "start": 814427, "stop": 814882, "strand": "-", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02939", "RefSeq:WP_013527049.1", "SO:0001217", "UniParc:UPI0001A8C365", "UniRef:UniRef100_C4XDZ0", "UniRef:UniRef50_Q4A5Y3", "UniRef:UniRef90_C4XDZ0" ], "nt": "ATGAAAGTAATATTGATTAAAGATTGTGATAAAGGTAAAGCCAATACAATTATTGAAGTTTCAGCTGGTTATGGTAATAACTTCTTAATTGCAAAAGGTTTTGCAGTTGTCTATAATGACAAGACTAAAAAAGAGCTTGAACAAAGACTTAGTGAGCTTACTAAAAATGAAATGGAAAAACGTTCAGAAGCTCTTGAACTTAAAGATCAAATTGAAAAATTAACTTTAAAATATACTTTAGATGCGCATATTGACAATAATGGAAATTTAATTGCTCATGGTGCTGTATCAACCAAAGATATTGTTAAAGATTTAGTCCAAAATGGCTATAAATTAGATAAATACGCAGTTCAAAAAGTTCATTTAGTATCTAATGGATTGCATGATATAGATGTTATTTTATATAAAGATATCATTGCAAAATTGAAAGTAGAAGTGATAATTAATGCCAAATAA", "aa": "MKVILIKDCDKGKANTIIEVSAGYGNNFLIAKGFAVVYNDKTKKELEQRLSELTKNEMEKRSEALELKDQIEKLTLKYTLDAHIDNNGNLIAHGAVSTKDIVKDLVQNGYKLDKYAVQKVHLVSNGLHDIDVILYKDIIAKLKVEVIINAK", "aa_hexdigest": "93fd4b1ac987040e6f87959cbecf40f6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C365", "ncbi_nrp_id": "WP_013527049.1", "uniref100_id": "UniRef100_C4XDZ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C365", "RefSeq:WP_013527049.1", "UniRef:UniRef100_C4XDZ0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDZ0", "uniref90_id": "UniRef90_C4XDZ0" }, "psc": { "uniref90_id": "UniRef90_C4XDZ0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_Q4A5Y3", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5Y3" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "EFPKPENHBP_1487", "locus": "GCA000186005_01476" }, { "type": "cds", "sequence": "contig_1", "start": 815277, "stop": 815777, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "TTGGATGCAATCGGTTCAGCTTTAGGAGTTTATGCTATAGCTAAAACATTTGATAAAGAAGCTTACATTTGCACAATGACTCAAGATGTTACAACTAAAAAAGCAATTGCTAAATACTTTGGTGAAGATAATCCAATTTTCATTAAAGCTCATCAAGCTAACACATTGACTGATGAAAATACACTTGTATTTTTAGTTGATAACTCAGCTCCAAATAGGACTGATAATCCAAAATGTGTTGAAAAAGCTAAAAACACAAATATCTTTATTTTAGACCACCATAGATTAAATTCTTCAGTTGACTTTTGTTCAAAAATTAATCGCCACATTGAGCCTTCAGCTTCAAGTGCTAGTGAAATAGTAACTGAATTAATGATGTTTTTAGAAAAGCAAGTTAAAGTTAAAAAGAACATTGCTCAAATGTTATTAAATGGTATTTATCTTGACACCTTACAATTTCAAAAACATGTTACTTCAAGAACATTTGAAGCAGCAAGTTGA", "aa": "MDAIGSALGVYAIAKTFDKEAYICTMTQDVTTKKAIAKYFGEDNPIFIKAHQANTLTDENTLVFLVDNSAPNRTDNPKCVEKAKNTNIFILDHHRLNSSVDFCSKINRHIEPSASSASEIVTELMMFLEKQVKVKKNIAQMLLNGIYLDTLQFQKHVTSRTFEAAS", "aa_hexdigest": "9b3686e0e379f9225b4e17774aef36eb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 151, "aa_cov": 0.8795180722891566, "hmm_cov": 0.7549668874172185, "evalue": 2.3995287075650266e-19, "score": 66.18602752685547, "start": 1, "stop": 146 } ], "seq_stats": { "molecular_weight": 18592.078499999996, "isoelectric_point": 6.955978584289551 }, "id": "EFPKPENHBP_1488", "locus": "GCA000186005_01477" }, { "type": "cds", "sequence": "contig_1", "start": 815844, "stop": 815999, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "ATGGAACAAGCTAAAAATGCTTTATTACAAGCTCAAAGTCGTGGTGGTGACCAAGTAGCTATTTTCTCAAGAAATAATTTACCTCGTTACTTTGGTTCAACAAGTGAAATCTTGCCAAATATCAACCGTGCAAGAATCAAAGCACTTGCTAATTGA", "aa": "MEQAKNALLQAQSRGGDQVAIFSRNNLPRYFGSTSEILPNINRARIKALAN", "aa_hexdigest": "e3af2c8c559a26432e1e324b3da8177b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 26, "aa_cov": 0.5098039215686274, "hmm_cov": 1.0, "evalue": 1.803740326909453e-09, "score": 34.28099060058594, "start": 2, "stop": 27 } ], "seq_stats": { "molecular_weight": 5644.3451, "isoelectric_point": 10.877960014343262 }, "id": "EFPKPENHBP_1489", "locus": "GCA000186005_01478" }, { "type": "cds", "sequence": "contig_1", "start": 816006, "stop": 816200, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "GTGTGTGATTACAACTTAGTTTATCGTCAATATAATACAAACGGTAAATTTCTTATTGTAACTAACAAGCAAGCTTTAGATAAAATGAGTCAAGTAAACTTTAAGTTTTTTGATGATTTACATAAAACACTAAAAAGTCAAAACATTATTATTTCAGTTTCTGCAGGTTTTGCATATGGAACAAAAAACTTATGA", "aa": "MCDYNLVYRQYNTNGKFLIVTNKQALDKMSQVNFKFFDDLHKTLKSQNIIISVSAGFAYGTKNL", "aa_hexdigest": "68e3dcb4fbc18fedaea0f8016eeccf5f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 62, "aa_cov": 0.96875, "hmm_cov": 0.967741935483871, "evalue": 3.5375637350597386e-18, "score": 62.42011642456055, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 7399.440099999999, "isoelectric_point": 9.41704196929932 }, "id": "EFPKPENHBP_1490", "locus": "GCA000186005_01479" }, { "type": "cds", "sequence": "contig_1", "start": 816537, "stop": 816833, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAACAAAAAATAATTTTATATTCTTTAATAGCAGTTGCTGGCGTTTTCCTAGCTGTAGGGTCAGTAATTTTGTATTTTACTCGTTCAGATATTTATAGCAGCATTTTTGGAATTTTTGGCATATGTCTTTCATTAATAATCGTTGGATTATTTGGAATTTTATCTGTTAAAAGTTTTTTATCAACTCAAGCTATAGTAAAAAAATCTTTTAACAGTTTCATTGATGAAATCATTAGTCATAATGCAATTGGAGTGCTTATTTTTGACAGTGAAGGCCAAATTCTTTGA", "aa": "MNLKQKIILYSLIAVAGVFLAVGSVILYFTRSDIYSSIFGIFGICLSLIIVGLFGILSVKSFLSTQAIVKKSFNSFIDEIISHNAIGVLIFDSEGQIL", "aa_hexdigest": "7b6b56416ddb61cfdc0263c92ea312e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10664.598699999986, "isoelectric_point": 7.878761863708497 }, "id": "EFPKPENHBP_1491", "locus": "GCA000186005_01480" }, { "type": "cds", "sequence": "contig_1", "start": 817086, "stop": 817268, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATATTGACCACAATAATAATGATTCTAAAAAACTTATAAATTCCTTTAATGAAATGCTTGATTTAAATATTGAAAACAAACTCCAATTTTTTATAGATGAATTTAGAAAAACAACAGATGATAAAATACTAAAATTTGTAGTTATCAAAAAAATGAGTAAGAAAAAAAATTCTTAA", "aa": "MANIDHNNNDSKKLINSFNEMLDLNIENKLQFFIDEFRKTTDDKILKFVVIKKMSKKKNS", "aa_hexdigest": "e48f5d5431332c899e8c89359da58c78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7148.2021, "isoelectric_point": 9.34754505157471 }, "id": "EFPKPENHBP_1492", "locus": "GCA000186005_01481" }, { "type": "cds", "sequence": "contig_1", "start": 817575, "stop": 817736, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACATAATGCTTTATCTAGTTAATAATAAATTAACCACTTACAATTCACAGTATATTTTTAGAGAAAAAGACAAACGCTATATCTTTGAAAAAAGCTCCCTAAATGAAATATTAAATAAAAATTTAAATGCAAACACAGGTCAAATTATAACCTGA", "aa": "MKNIMLYLVNNKLTTYNSQYIFREKDKRYIFEKSSLNEILNKNLNANTGQIIT", "aa_hexdigest": "bfacfb99c1df866093beadbb76364c8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6345.262999999998, "isoelectric_point": 9.630754661560058 }, "id": "EFPKPENHBP_1493", "locus": "GCA000186005_01482" }, { "type": "cds", "sequence": "contig_1", "start": 817773, "stop": 817985, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAATTTGAAGCAAATCTAGAATTTTATAACAATTTTTCAGATTGTTTAAACTTTATAGATAGCTTAACAAATGTTTTTCCAAAAGATAACACTTTTTACAAAAAAACCTATTTTACAGTTAATAACGACAATTTGTTCTATATGCAAAACTCAAATCTTTATAAAATAATTTTAAATAAAAGACATCTTGAAAATATGAATACTTGA", "aa": "MEKFEANLEFYNNFSDCLNFIDSLTNVFPKDNTFYKKTYFTVNNDNLFYMQNSNLYKIILNKRHLENMNT", "aa_hexdigest": "7cc9564dcdc57bd2c423cbda59968218", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8537.515099999997, "isoelectric_point": 5.635949897766112 }, "id": "EFPKPENHBP_1494", "locus": "GCA000186005_01483" }, { "type": "cds", "sequence": "contig_1", "start": 818657, "stop": 818821, "strand": "-", "frame": 3, "gene": null, "product": "HTH-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527053.1", "SO:0001217", "UniParc:UPI0001F59859", "UniRef:UniRef100_A0AB32XCG9", "UniRef:UniRef50_C4XEC1", "UniRef:UniRef90_A0AB32XCG9" ], "nt": "TTGAAAAAGATTATATTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCATTAAGAAATAAAGGAATAATGATTAATCATAAAAAGGTTCAAAAATTAATAAAATCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAA", "aa": "MKKIIFDLFNYNKGLYGYRRITFALRNKGIMINHKKVQKLIKSLNLFGKTLRKK", "aa_hexdigest": "cecf8bcc0feec8340518c5607ffeb5a2", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59859", "ncbi_nrp_id": "WP_013527053.1", "uniref100_id": "UniRef100_A0AB32XCG9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59859", "RefSeq:WP_013527053.1", "UniRef:UniRef100_A0AB32XCG9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCG9", "uniref90_id": "UniRef90_A0AB32XCG9" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCG9", "uniref50_id": "UniRef50_C4XEC1" }, "pscc": { "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1" ], "product": "HTH-like domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1495", "locus": "GCA000186005_01484" }, { "type": "cds", "sequence": "contig_1", "start": 818937, "stop": 819071, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5985A", "UniRef:UniRef100_A0AB32XD23", "UniRef:UniRef50_A0AB32XD23", "UniRef:UniRef90_A0AB32XD23" ], "nt": "ATGGAAGATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTTAAATTGGAAAACGCGATAGCAAAAAAAGTGAGCGTTTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MEDKDLLIKQLKEENKILKLENAIAKKVSVLVQLKDSLTKKNSK", "aa_hexdigest": "61d7f2fbdd701153f3a3f94a31e5d7c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5985A", "uniref100_id": "UniRef100_A0AB32XD23", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5985A", "UniRef:UniRef100_A0AB32XD23" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD23", "uniref90_id": "UniRef90_A0AB32XD23" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD23", "uniref50_id": "UniRef50_A0AB32XD23" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD23" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5066.009200000001, "isoelectric_point": 9.574538230895993 }, "id": "EFPKPENHBP_1496", "locus": "GCA000186005_01485" }, { "type": "cds", "sequence": "contig_1", "start": 819344, "stop": 819478, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ4", "UniRef:UniRef90_C4XDZ4" ], "nt": "ATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGA", "aa": "MIQKFRPELVGRFKDISFLDKFNAKQVKALINKKNQRRRNQYLL", "aa_hexdigest": "70c4483868b5529ad3f62102c7b32ddb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 819206, "stop": 819511, "observations": { "mutation": [ 819476 ], "stop": [ 819476 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ4", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 1.0, "score": 86.7, "evalue": 3.64e-21, "gene_start": 1, "gene_end": 44, "reference_start": 47, "reference_end": 90, "reference_sequence": "MDECKFINYINVGYDCSKLILIFQSNVSQDNYSNNKDKNSQKEQEKMIQKFRPELVGRFKDISFLDKFNAKQVKALINKKNQRRRNQYLLWKSYFKFIYRY" }, "paralog": false, "identity": 0.9801980198019802, "score": 203.0, "evalue": 1.35e-69, "description": "Internal stop codon at 819476. Nonsense mutation around 819476.", "psc": { "uniref90_id": "UniRef90_C4XDZ4", "uniref50_id": "UniRef50_C4XDZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ4", "UniRef:UniRef50_C4XDZ4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5394.352700000001, "isoelectric_point": 11.112367057800292 }, "id": "EFPKPENHBP_1497", "locus": "GCA000186005_01486" }, { "type": "cds", "sequence": "contig_1", "start": 819681, "stop": 819950, "strand": "+", "frame": 3, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "nt": "TTGTTGTATAATACAAAAACTATGGATCAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGA", "aa": "MLYNTKTMDQNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLV", "aa_hexdigest": "d1aa701c23c2a49332ae04a3e21d5dd1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0022A714CF", "query_cov": 0.8651685393258427, "subject_cov": 0.8850574712643678, "identity": 0.61, "score": 103.0, "evalue": 3.17e-27, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI0022A714CF" }, "pscc": { "uniref50_id": "UniRef50_UPI0022A714CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "product": "HAD family hydrolase" }, "genes": [], "id": "EFPKPENHBP_1498", "locus": "GCA000186005_01487" }, { "type": "cds", "sequence": "contig_1", "start": 820104, "stop": 820541, "strand": "+", "frame": 3, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5", "UniRef:UniRef90_C4XDZ5" ], "nt": "ATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCAAAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAA", "aa": "MHGIKRSKNFNFNNQKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND", "aa_hexdigest": "e2949967b0f2ab7fb5d976085ac27433", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 819804, "stop": 820541, "observations": { "mutation": [ 819948, 820074 ], "stop": [ 819948, 820074 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ5", "query_cov": 1.0, "subject_cov": 0.48494983277591974, "identity": 0.993, "score": 282.0, "evalue": 6.45e-94, "gene_start": 1, "gene_end": 145, "reference_start": 155, "reference_end": 299, "reference_sequence": "MGSFLFANNIHYFLLYNTKTMDKNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLVWQRTFKKDTANKIQKFAEENKILYVIFDEEGETIYVHNAFDYWINKLFLSKKMHGIKRSKNFNFNNHKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND" }, "paralog": false, "identity": 0.9877551020408163, "score": 474.0, "evalue": 7.01e-172, "description": "Internal stop codon at 819948, 820074. Nonsense mutation around 819948, 820074.", "psc": { "uniref90_id": "UniRef90_C4XDZ5", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ5", "UniRef:UniRef50_C4XDZ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5" ], "product": "COF family HAD hydrolase protein" } }, "id": "EFPKPENHBP_1499", "locus": "GCA000186005_01488" }, { "type": "cds", "sequence": "contig_1", "start": 820534, "stop": 821082, "strand": "+", "frame": 1, "gene": null, "product": "deoxyribonuclease IV", "db_xrefs": [ "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_C4XDZ6" ], "nt": "ATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGCTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTGGGCGCTCCTCAGAATGCTAAAAGAGTTGATACAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAGTTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGA", "aa": "MIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDTKVYKYEEYKANYSNLIKPEDIVVHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHV", "aa_hexdigest": "16a1bf1126283fd3cab12f0ebe39ca55", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 820480, "stop": 821373, "observations": { "mutation": [ 821080 ], "stop": [ 821080 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ6", "query_cov": 1.0, "subject_cov": 0.6127946127946128, "identity": 1.0, "score": 363.0, "evalue": 3.14e-125, "gene_start": 1, "gene_end": 182, "reference_start": 19, "reference_end": 200, "reference_sequence": "MVVCIVILFQVRARKEAIMIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDTKVYKYEEYKANYSNLIKPEDIVVHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHVWDAGYNIKNYQEFKSEINKYNLLKHIKVIHLNDSKNDLNSHKDRHENIDKGFIGIDTLAKFVHDKDFDNVPIILETPIPENGPIYDQEIAALLAYKK" }, "paralog": false, "identity": 0.9932659932659933, "score": 590.0, "evalue": 2.85e-217, "description": "Internal stop codon at 821080. Nonsense mutation around 821080.", "psc": { "uniref90_id": "UniRef90_C4XDZ6", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ6", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "EFPKPENHBP_1500", "locus": "GCA000186005_01489" }, { "type": "cds", "sequence": "contig_1", "start": 821921, "stop": 822241, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGA", "aa": "MYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVR", "aa_hexdigest": "3cf7fa56839314ecec6b0798db4808aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12384.719499999985, "isoelectric_point": 9.474999046325685 }, "id": "EFPKPENHBP_1501", "locus": "GCA000186005_01490" }, { "type": "cds", "sequence": "contig_1", "start": 822698, "stop": 823978, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7", "UniRef:UniRef90_C4XDZ7" ], "nt": "ATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGA", "aa": "MVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDND", "aa_hexdigest": "b08ef718b132cee0c4260278337a4d56", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 822398, "stop": 824005, "observations": { "mutation": [ 822407, 822452, 822614, 822626, 823976 ], "stop": [ 822407, 822452, 822614, 822626, 823976 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ7", "query_cov": 1.0, "subject_cov": 0.6016949152542372, "identity": 1.0, "score": 684.0, "evalue": 1.41e-241, "gene_start": 1, "gene_end": 426, "reference_start": 274, "reference_end": 699, "reference_sequence": "MIINTKLDINIYIKMYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVRWFGSIFKNHYFAWFKSYTRVDYWILRKRIISFVWLTLLFVVLIYHVVLVSFRVENIWYYSTADVKNIYLKGWYYTFTNQADLLDGKSVDYPVAYLNIGFLLDTLFNLFYVVTFSPYLAIISAFLIWTLAWLFLVTLNPIKYFKNINEHKKTIVMVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDNDWVSPILSDN" }, "paralog": false, "identity": 0.9906542056074766, "score": 932.0, "evalue": 0.0, "description": "Internal stop codon at 822407, 822452, 822614, 822626, 823976. Nonsense mutation around 822407, 822452, 822614, 822626, 823976.", "psc": { "uniref90_id": "UniRef90_C4XDZ7", "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ7", "UniRef:UniRef50_C4XDZ7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 49151.044900000095, "isoelectric_point": 4.635867881774902 }, "id": "EFPKPENHBP_1502", "locus": "GCA000186005_01491" }, { "type": "cds", "sequence": "contig_1", "start": 824125, "stop": 824238, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGA", "aa": "MKPIVESVEEEYKEYNEIIFLEVDAEKAQLFREINNR", "aa_hexdigest": "d035d9e1e33c59dcdacd73cadc0fb5e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4517.0309, "isoelectric_point": 4.431986427307129 }, "id": "EFPKPENHBP_1503", "locus": "GCA000186005_01492" }, { "type": "cds", "sequence": "contig_1", "start": 824482, "stop": 824865, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGA", "aa": "MHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKF", "aa_hexdigest": "fee39558eb5b77120edeffab94938226", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15099.314499999984, "isoelectric_point": 9.176897239685058 }, "id": "EFPKPENHBP_1504", "locus": "GCA000186005_01493" }, { "type": "cds", "sequence": "contig_1", "start": 825100, "stop": 826083, "strand": "+", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_O84898", "UniRef:UniRef90_C4XDZ9" ], "nt": "ATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTTAAAACATATGTTAATATCTTTAACGCTCCTTTAGAAGAATTAGAAAAAGTTGTTTCTAAAAAAATAGCTTTAGAAATCAAAAAACTTTATGAAAGTAAGAAATAA", "aa": "MTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTFKTYVNIFNAPLEELEKVVSKKIALEIKKLYESKK", "aa_hexdigest": "ccd5083e682d8cb61b3a97a0c0d70a26", "start_type": "ATG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "5-prime", "pseudogene": { "start": 824800, "stop": 826083, "observations": { "mutation": [ 824863, 824998 ], "stop": [ 824863, 824998 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ9", "query_cov": 1.0, "subject_cov": 0.5696864111498258, "identity": 1.0, "score": 624.0, "evalue": 1.31e-221, "gene_start": 1, "gene_end": 327, "reference_start": 248, "reference_end": 574, "reference_sequence": "MTKEEIITSLKNVSTAPGIYLWKDKNDQVIYVGKAKNLRKRMHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKFWQEKFNKIKSILSFKDNSYINEIKEKMFKEAETEHFELARDLKLSWEYLKKLNEEQIIELKDIHNLDVFAYKTVDNIIYMTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTFKTYVNIFNAPLEELEKVVSKKIALEIKKLYESKK" }, "paralog": false, "identity": 0.9953161592505855, "score": 827.0, "evalue": 8.14e-304, "description": "Internal stop codon at 824863, 824998. Nonsense mutation around 824863, 824998.", "psc": { "uniref90_id": "UniRef90_C4XDZ9", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_O84898", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ9", "UniRef:UniRef50_O84898", "KEGG:K03703", "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_O84898", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O84898" ], "product": "UvrABC system protein C" } }, "id": "EFPKPENHBP_1505", "locus": "GCA000186005_01494" }, { "type": "cds", "sequence": "contig_1", "start": 826479, "stop": 826904, "strand": "+", "frame": 3, "gene": null, "product": "6-phospho-beta-glucosidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE01", "UniRef:UniRef90_C4XE01" ], "nt": "TTGTTCAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAATAATAAAAAAGTTGTTAAATACTTTTTAAATTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTATGCTAAAGTCAAAGAAATAATTAAAAATAATTACAATCAGATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGTGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGA", "aa": "MFKELKRRKIKTIVTLCHYEMPLHLALKYNGFNNKKVVKYFLNYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYAYAKVKEIIKNNYNQIQVGSMVLAMPFYTKSAKVEDQILKMEKE", "aa_hexdigest": "31ca9bdaaede8a489d8725233dc27a84", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 826377, "stop": 827063, "observations": { "mutation": [ 826410, 826902 ], "stop": [ 826410, 826902 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE01", "query_cov": 1.0, "subject_cov": 0.618421052631579, "identity": 0.9790000000000001, "score": 278.0, "evalue": 1.71e-93, "gene_start": 1, "gene_end": 141, "reference_start": 35, "reference_end": 175, "reference_sequence": "MGIKVLRISIAWSRIFPKGDESKPNQKGLEFYLKLFKELKRRKIKTIVTLCHYEMPLHLALKYNGFSNKKVVKYFLNYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYASAKVKEIIKNNYNQIQVGSMVLAMPFYTKSAKVEDQILKMEKEWQNLYFTDVLARGVYPKYLDNYFLKNNIKINKTKEELEVIKNNIVDYIGFSYC" }, "paralog": false, "identity": 0.9824561403508771, "score": 450.0, "evalue": 8.84e-164, "description": "Internal stop codon at 826410, 826902. Nonsense mutation around 826410, 826902.", "psc": { "uniref90_id": "UniRef90_C4XE01", "product": "6-phospho-beta-glucosidase", "uniref50_id": "UniRef50_C4XE01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE01", "UniRef:UniRef50_C4XE01" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE01" ], "product": "6-phospho-beta-glucosidase" } }, "id": "EFPKPENHBP_1506", "locus": "GCA000186005_01495" }, { "type": "cds", "sequence": "contig_1", "start": 827165, "stop": 827266, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1", "UniRef:UniRef90_A0AB32XCF1" ], "nt": "ATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGA", "aa": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSE", "aa_hexdigest": "bf6438b1ab969c940bd1670c9fe5b05a", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 827165, "stop": 827332, "observations": { "mutation": [ 827264 ], "stop": [ 827264 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCF1", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 70.9, "evalue": 1.2e-15, "gene_start": 1, "gene_end": 33, "reference_start": 1, "reference_end": 33, "reference_sequence": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSEWISNNYFRNKVHIIFKSEGGKF" }, "paralog": false, "identity": 0.9818181818181818, "score": 115.0, "evalue": 1.08e-35, "description": "Internal stop codon at 827264. Nonsense mutation around 827264.", "psc": { "uniref90_id": "UniRef90_A0AB32XCF1", "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCF1", "UniRef:UniRef50_A0AB32XCF1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3992.4436000000005, "isoelectric_point": 4.676394081115721 }, "id": "EFPKPENHBP_1507", "locus": "GCA000186005_01496" }, { "type": "cds", "sequence": "contig_1", "start": 827402, "stop": 827554, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTGTTGTTAACTTTATTTTAGTTTGCTTAATATATTTAATTGGATTGTTTCTATTTCCTCTCACTTATTTCAAATGTAAAAACTATGAAACAGATTTAAAAAACAATGTTAAGTTCAATGTAGACTTATATGGAGAAGCTAAATAA", "aa": "MKLVVNFILVCLIYLIGLFLFPLTYFKCKNYETDLKNNVKFNVDLYGEAK", "aa_hexdigest": "70377fa20cdf25e2d7eb34176df54173", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5910.058200000002, "isoelectric_point": 8.638263893127444 }, "id": "EFPKPENHBP_1508", "locus": "GCA000186005_01497" }, { "type": "cds", "sequence": "contig_1", "start": 827646, "stop": 827912, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGA", "aa": "MITLSSLKVALKIKLLSTKAKIKGIIIIAAYITIWVVLTNVFALVVVSRFRKLSHDANAPIPIVAILAAKAIQDNFFILSIIDNCSSI", "aa_hexdigest": "7af78ef7a1ecb8640d0d73e7f2e97d05", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.599199999993, "isoelectric_point": 10.106337928771971 }, "id": "EFPKPENHBP_1509", "locus": "GCA000186005_01498" }, { "type": "cds", "sequence": "contig_1", "start": 828282, "stop": 828773, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "ATGCCTGAAGTAGTTTTAGTAGATAATGAAAAAGAAAAGAAACAAATATTAAAAGATCTTTTAACAAATGTTTATGTTAATGACATTGTTGAGAGACATAAACTTAATAGTACTCAAGATGTTTTAAACATAGTTGAATATCTTGCTTCAATAGTTTCATCTCAATGTAATTCAAGTAGAATTGCCAACTCTTTTAATTCTTTAAATAATAAAAAGACTTCTACTTATCAAATAGATAAAATATTAGATGCACTAGAAGATTCTTTCTTAATAGATAAAATAAGAAAATATGATATTAAAGGTTTAAAACTTCTTTCGGGTCAATATAAATACTATTTTGTTGACAACGGATTAAGAAATGTAGCTCTCAATTTTAGAAATCTAGGACAAGGTCAATTAATCGAAAACGCCTTTTTTAATGCTCTAAAAATAAATTACTTCGATATTAGTTATGGGGCAATTAATCAAAAAAATAATATTAATAATCAATGA", "aa": "MPEVVLVDNEKEKKQILKDLLTNVYVNDIVERHKLNSTQDVLNIVEYLASIVSSQCNSSRIANSFNSLNNKKTSTYQIDKILDALEDSFLIDKIRKYDIKGLKLLSGQYKYYFVDNGLRNVALNFRNLGQGQLIENAFFNALKINYFDISYGAINQKNNINNQ", "aa_hexdigest": "d1f872c9c0f9a504df55fe1e5a861e93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 127, "aa_cov": 0.7361963190184049, "hmm_cov": 0.952755905511811, "evalue": 1.7183570316763527e-13, "score": 46.9279670715332, "start": 27, "stop": 146 } ], "seq_stats": { "molecular_weight": 18749.07219999999, "isoelectric_point": 8.526024436950681 }, "id": "EFPKPENHBP_1510", "locus": "GCA000186005_01499" }, { "type": "cds", "sequence": "contig_1", "start": 828798, "stop": 828980, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTAAATTCATTTCAAGATCAAGATAATATTGATATTTATGTTACTGGCAGTAATTCTAAATTCTTATCAAAAGATATAGCTACTACTTTTGCAGGTCGAGGTATGGAGATTAATGTTTTGCCTTTATCATTTCAAGAATATTTAAACTTTAGTAATCTTGATATAAATGAAGCTTGA", "aa": "MVLNSFQDQDNIDIYVTGSNSKFLSKDIATTFAGRGMEINVLPLSFQEYLNFSNLDINEA", "aa_hexdigest": "e17668e6b138d8966b636308086a5ccf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6750.442699999998, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_1511", "locus": "GCA000186005_01500" }, { "type": "cds", "sequence": "contig_1", "start": 829080, "stop": 829289, "strand": "-", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "nt": "ATGAATAAAGTTATTAAAAGAGACAATTATTTAAATATGCTAATTAATAAAATCAATAACAAAAAAATTAAAATAATTACAGGTATAAGAAGAGTAGGCAAAAGTTTTTTACTCTTCAATTTATTCTATAGTTATTTATTAGCTAATAAGGTGGAAGCTGACCAAATAATAACAATAAAACTTGACCGAGTTGAAAATGAAGAACTTTGA", "aa": "MNKVIKRDNYLNMLINKINNKKIKIITGIRRVGKSFLLFNLFYSYLLANKVEADQIITIKLDRVENEEL", "aa_hexdigest": "178371902963c983f955048731aec3f6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFEF55", "query_cov": 0.9420289855072463, "subject_cov": 0.9154929577464789, "identity": 0.615, "score": 80.5, "evalue": 1.28e-18, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A4Y8VNC6" }, "pscc": { "uniref50_id": "UniRef50_A0A4Y8VNC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1512", "locus": "GCA000186005_01501" }, { "type": "cds", "sequence": "contig_1", "start": 829538, "stop": 829738, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCV5", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_A0AB32XCV5", "UniRef:UniRef90_UPI000FF82D94" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTTCAAATAGAAGAAGTTAGAAACAATTATCAAAACTTTTTATGTTTGTTTAGCGAAGCGAGACTAAGAGTTAGATTGTGTAGAAAAGAAAACATGCATTTTGGCGATGAATTACAAATAGACGCTTGTATCTATGAATCATTTGATAGGACTATGAGTGCATACTATCATTAA", "aa": "MIKIAELLKVQIEEVRNNYQNFLCLFSEARLRVRLCRKENMHFGDELQIDACIYESFDRTMSAYYH", "aa_hexdigest": "39ec9fd6052db5fe43ebc4bb17339f4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.985, "score": 135.0, "evalue": 1.49e-40, "valid": true, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 829538, "stop": 829957, "observations": { "mutation": [ 829879 ], "stop": [ 829879 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCV5", "query_cov": 1.0, "subject_cov": 0.4748201438848921, "identity": 1.0, "score": 138.0, "evalue": 1.12e-40, "gene_start": 1, "gene_end": 66, "reference_start": 74, "reference_end": 139, "reference_sequence": "MEEYTEYIKNKINGIKNVNDATIKSFWKEIRIVDYSHVGYEKQISYQTLVNRLTSLGFLTIFSHKKTRRKVRKMIKIAELLKVQIEEVRNNYQNFLCLFSEARLRVRLCRKENMHFGDELQIDACIYESFDRTMSAYYH" }, "paralog": false, "identity": 0.9928057553956835, "score": 277.0, "evalue": 6.89e-98, "description": "Internal stop codon at 829879. Nonsense mutation around 829879.", "psc": { "uniref90_id": "UniRef90_A0AB32XCV5", "uniref50_id": "UniRef50_A0AB32XCV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCV5", "UniRef:UniRef50_A0AB32XCV5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCV5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8007.170799999997, "isoelectric_point": 6.060423469543457 }, "id": "EFPKPENHBP_1513", "locus": "GCA000186005_01502" }, { "type": "cds", "sequence": "contig_1", "start": 830334, "stop": 830594, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTATTGATTATTTTTTAGATTGCTTAATTATTTCCTACAATAAATTAATAGATGTAAATAAAAAAGATTATTTAGAAAATCAAATAATCGAAATTTTAACTACTAGCTTAATTCCTTTATCAACTAAAGAAATAAGTGAACGACTTGTTTCAATTTCAAGAAGAACATTAGAAAGAAAACTTAAAGTCTTATTAGAACAAAATAAAATCAAAAAAGTTGGTCAATATAAAAATGCTAAATATAAATTAACATAA", "aa": "MPFIDYFLDCLIISYNKLIDVNKKDYLENQIIEILTTSLIPLSTKEISERLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT", "aa_hexdigest": "3ebfb0117e7a39f810d8dc0f7f8bffd5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10177.961699999987, "isoelectric_point": 9.519095420837402 }, "id": "EFPKPENHBP_1514", "locus": "GCA000186005_01503" }, { "type": "cds", "sequence": "contig_1", "start": 830616, "stop": 831380, "strand": "-", "frame": 2, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05", "UniRef:UniRef90_C4XE05" ], "nt": "ATGGTTAAAAAAGAAAAAAGGTTTTTTGATTATTCATTTTTAAAGAACATTATTAATAATGAAATTAACAAAAAATTAAAACAAATTTATGAGTTAAATGGACTTATAAATGATTTTTCTAATATTCCAGAACAAACTAAAAATACTTTAATTAAAAATAGTATTTTTCAAAGTGTTACTTCTTCAAACAAAATTGAAAATATTGTTTCAACTACAAACAAAATTGAAAATATTATTTTTAATAATAAAAAATATACAGGCGAAAACGAATTAGAAATTCAAGGTTATAATGATGTATTAAATATTATTTTAAATAATTTTGATAATATAGATATTACTAAATCAAACATCTTAAATTTACATAATCAATTATATAAATATAGTAATAAAACTTATTTAAAAGGAAAGTTTAAAGAAATGAACAATTATATTTTTTCAATTGACCAAAACAACTATAAAACTGTTATATTTGAACCGGTTGATCATTCTTTGGTTGATGATTACATAACAAAAATATGTGATGAATACAATAAAAGAAATGATGAAAAAATAATTGATCCTTTAATACTAATTCCAACATTTATTTTGGATTTTTTATGTATTCACCCTTTTAGTGATGGCAATGGTAGAATTTCAAGATTATTAACTAACTGTTTATTATTTCAAAATGATTTTATGATTAATAAATACATTAGTTTTGAAAAAATCATAGAAGAAAATTTAAATCAATATTATTTCACTTTAAGAAAAAGCTCAACTAATTGA", "aa": "MVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEENLNQYYFTLRKSSTN", "aa_hexdigest": "ef0bf0dce3874e5ff86f93460df0962a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 830334, "stop": 831389, "observations": { "mutation": [ 830618 ], "stop": [ 830618 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE05", "query_cov": 1.0, "subject_cov": 0.7236467236467237, "identity": 1.0, "score": 472.0, "evalue": 2.27e-166, "gene_start": 1, "gene_end": 254, "reference_start": 4, "reference_end": 257, "reference_sequence": "MIIMVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEENLNQYYFTLRKSSTNWQYSQNDYLPFIDYFLDCLIISYNKLIDVNKKDYLENQIIEILTTSLIPLSTKEISERLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT" }, "paralog": false, "identity": 0.9943019943019943, "score": 669.0, "evalue": 2.12e-246, "description": "Internal stop codon at 830618. Nonsense mutation around 830618.", "psc": { "uniref90_id": "UniRef90_C4XE05", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE05", "UniRef:UniRef50_C4XE05" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05" ], "product": "Fido domain-containing protein" } }, "id": "EFPKPENHBP_1515", "locus": "GCA000186005_01504" }, { "type": "cds", "sequence": "contig_1", "start": 831795, "stop": 832652, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50", "UniRef:UniRef90_UPI000FEE809E" ], "nt": "ATGCTTAAAAGCGCAGGTTTAATTCTAAAAGATCCTGAAACCAATAAAGAAGGAATAACTATAGCAGCTATTTTGCTTTTTGGAAAAGATCAAACTATTATGTCTGTTCTTCCTCAATATCGTACAGATGCTATTTTTAGAGTTAAAAATAAAGATAGATATGATGATAGAGAAATAGTAGCAACCAATTTAATCGATAGCTATGAGAAATTAATTAATTTTGGTGAAAAACACTTAAGCGATATTTTTGTTTTAGATGGTATTGTAAGTGTTAGTGCTCGTAATTTGATACTTAGAGAAATCATTTCTAATACACTAGCTCATAGAAAATATGCAAGCAATATGACAGCTACTATTGTCATTGATGAAAACAATATAACTGTTGAAAATGATAATAGTTCAAGCAGTAAAGAGGAATTAGATCCTAAAACCTTTAGACCAAATCCAAAAAATCCACCAATTTCAAAAGTTTTTAGAGAAATTGGTTTAGCTGATGAATTAGGTTCAGGAATTAGAAAAACTTATAAATATACAAAACTTTATTCAAATAATAAACCTACATTTTTGGATAGTAACATATTTAAAACGATTATTCCTATTCCAAAAATTGCTACATTAAAAGTTGGAAAAATGACCTCTAATATAGATACAGATAATAAAAATATTACAAACAAAAAACAATTAAATATTGAAAGAATTTTCAATTTAGTTAAAGAAAATTCTACAATAAAAAGAAGTGAAATTGCAGCAATTTTAAATTTAACTGAAAAAACTATTTCAAGATATATTAGAGAAATAAAAAACATAAAATATGTTGGAAAAGGTAAAAATGGTTATTGGGAAATTATTGATAAATAG", "aa": "MLKSAGLILKDPETNKEGITIAAILLFGKDQTIMSVLPQYRTDAIFRVKNKDRYDDREIVATNLIDSYEKLINFGEKHLSDIFVLDGIVSVSARNLILREIISNTLAHRKYASNMTATIVIDENNITVENDNSSSSKEELDPKTFRPNPKNPPISKVFREIGLADELGSGIRKTYKYTKLYSNNKPTFLDSNIFKTIIPIPKIATLKVGKMTSNIDTDNKNITNKKQLNIERIFNLVKENSTIKRSEIAAILNLTEKTISRYIREIKNIKYVGKGKNGYWEIIDK", "aa_hexdigest": "247aaeb9d07c8ab815028e24a9562fe0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEE809E", "query_cov": 1.0, "subject_cov": 0.8431952662721893, "identity": 0.996, "score": 541.0, "evalue": 1.91e-193, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_R9RA50" }, "pscc": { "uniref50_id": "UniRef50_R9RA50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50" ], "product": "Helix-turn-helix type 11 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1516", "locus": "GCA000186005_01505" }, { "type": "cds", "sequence": "contig_1", "start": 832881, "stop": 833216, "strand": "-", "frame": 2, "gene": null, "product": "helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF015A0", "UniRef:UniRef90_UPI000FF015A0" ], "nt": "ATGAATAAAGAAGAATTGAAAAAACTTTTAATCCAAGGTGAAGGCGAAGAAATAGAATTCAAAACATGCAAAGATGCCTTATCTAAAAGTGTTTTTGATACCGTTTCAGCTTTTAGCAATAAAAATGGAGGTCATCTAATTTTAGGTATTGGAGACAATGGTGAAGTTTTAGGTGTAGACAAAACTAAAGTTAAAAAAATAGTAAATGACTTCTTGAATTCAATTCATGATTCAACTAAAATTAATCCTCCTTTATTTATTGAACCTGAAGTAATAGATTTTGATGATAAAAAAATAATTTATATTTATGTTCCAGTTGGAGTAAATGTTTGTTGA", "aa": "MNKEELKKLLIQGEGEEIEFKTCKDALSKSVFDTVSAFSNKNGGHLILGIGDNGEVLGVDKTKVKKIVNDFLNSIHDSTKINPPLFIEPEVIDFDDKKIIYIYVPVGVNVC", "aa_hexdigest": "3b9ecac3630c9d99ab28510e6fa7eb9a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 832772, "stop": 833216, "observations": { "insertion": [ 832793 ], "mutation": [ 832811, 832883 ], "stop": [ 832811, 832883 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF015A0", "query_cov": 1.0, "subject_cov": 0.7551020408163265, "identity": 1.0, "score": 217.0, "evalue": 3.89e-71, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MNKEELKKLLIQGEGEEIEFKTCKDALSKSVFDTVSAFSNKNGGHLILGIGDNGEVLGVDKTKVKKIVNDFLNSIHDSTKINPPLFIEPEVIDFDDKKIIYIYVPVGVNVCWHNGRIFERSFEGDVDITNKDELVWKLFQKKKVFVL" }, "paralog": false, "identity": 0.9863945578231292, "score": 283.0, "evalue": 1.71e-99, "description": "Internal stop codon at 832811, 832883. Frameshift due to insertion around 832793. Nonsense mutation around 832811, 832883.", "psc": { "uniref90_id": "UniRef90_UPI000FF015A0", "product": "helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_UPI000FF015A0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF015A0", "UniRef:UniRef50_UPI000FF015A0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF015A0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF015A0" ], "product": "helix-turn-helix domain-containing protein" } }, "id": "EFPKPENHBP_1517", "locus": "GCA000186005_01506" }, { "type": "cds", "sequence": "contig_1", "start": 833790, "stop": 834260, "strand": "+", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANL", "aa_hexdigest": "adc8cbbec5672ec1b725519e74a278c1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 833727, "stop": 834509, "observations": { "mutation": [ 834258, 834279 ], "stop": [ 834258, 834279 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 1.0, "score": 304.0, "evalue": 6.56e-103, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9923076923076923, "score": 501.0, "evalue": 6.38e-183, "description": "Internal stop codon at 834258, 834279. Nonsense mutation around 834258, 834279.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "EFPKPENHBP_1518", "locus": "GCA000186005_01507" }, { "type": "cds", "sequence": "contig_1", "start": 834740, "stop": 834931, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526872.1", "SO:0001217", "UniParc:UPI00000C0690", "UniRef:UniRef100_C4XF95", "UniRef:UniRef50_C4XF95", "UniRef:UniRef90_C4XF95" ], "nt": "ATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAG", "aa": "MKYKEGQLILVNYKFAQKFLSSSVGDDFLYLGLQNRKNLAKRLSQIMNIPKLTPEFHCIAKGS", "aa_hexdigest": "4e10fe405d634def018d3530d31e48fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C0690", "ncbi_nrp_id": "WP_013526872.1", "uniref100_id": "UniRef100_C4XF95", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0690", "RefSeq:WP_013526872.1", "UniRef:UniRef100_C4XF95" ] }, "ips": { "uniref100_id": "UniRef100_C4XF95", "uniref90_id": "UniRef90_C4XF95" }, "psc": { "uniref90_id": "UniRef90_C4XF95", "uniref50_id": "UniRef50_C4XF95" }, "pscc": { "uniref50_id": "UniRef50_C4XF95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF95" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.454599999999, "isoelectric_point": 9.779418754577634 }, "id": "EFPKPENHBP_1519", "locus": "GCA000186005_01508" }, { "type": "cds", "sequence": "contig_1", "start": 835192, "stop": 835365, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGNLGLEHNVLTAND", "aa_hexdigest": "2e7232a416963aea6061be918ee46d1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.762199999999, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_1520", "locus": "GCA000186005_01509" }, { "type": "cds", "sequence": "contig_1", "start": 835483, "stop": 835623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "EFPKPENHBP_1521", "locus": "GCA000186005_01510" }, { "type": "cds", "sequence": "contig_1", "start": 835729, "stop": 836070, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENMFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ca03355bf950c1fafc7488b9f7c761da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13355.14579999998, "isoelectric_point": 9.442893791198731 }, "id": "EFPKPENHBP_1522", "locus": "GCA000186005_01511" }, { "type": "cds", "sequence": "contig_1", "start": 836410, "stop": 836631, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAA", "aa": "MVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ", "aa_hexdigest": "4e375f5225f369e001680770108e444f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 836161, "stop": 836631, "observations": { "mutation": [ 836215, 836374 ], "stop": [ 836215, 836374 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.46794871794871795, "identity": 1.0, "score": 147.0, "evalue": 3.96e-44, "gene_start": 1, "gene_end": 73, "reference_start": 84, "reference_end": 156, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9807692307692307, "score": 295.0, "evalue": 1.61e-104, "description": "Internal stop codon at 836215, 836374. Nonsense mutation around 836215, 836374.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8364.290099999997, "isoelectric_point": 8.664953804016115 }, "id": "EFPKPENHBP_1523", "locus": "GCA000186005_01512" }, { "type": "cds", "sequence": "contig_1", "start": 836631, "stop": 836927, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTCCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGA", "aa": "MGDKKKKILKISLSAVAILLAAIIIFLALAIIFNVKRLKSFKMVSIKDFFAQTKASIYAFPKQFLISYAFLLIASIAGISYLFYKNKLKTSLLKPNKY", "aa_hexdigest": "a7ee8fb1bcb6146dfc81f181ec136612", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11069.498099999988, "isoelectric_point": 10.299291801452636 }, "id": "EFPKPENHBP_1524", "locus": "GCA000186005_01513" }, { "type": "cds", "sequence": "contig_1", "start": 837066, "stop": 837368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGA", "aa": "MNEPESDINSVVLGGVGSGKTQRVLLPNIIYNSHLRYKYRANFCIPDPKKEIIKMVGKKLEKQGYKIYAVDFSDTKKSVGWNPLSFAYKLAHRVPEGHPK", "aa_hexdigest": "6390e01b408d03e3933cb69dff4b3dd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11349.04659999999, "isoelectric_point": 9.68065319061279 }, "id": "EFPKPENHBP_1525", "locus": "GCA000186005_01514" }, { "type": "cds", "sequence": "contig_1", "start": 837453, "stop": 837593, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTGA", "aa": "MWVDNAKLIVNVVAQFLLLLSIDRPDLLPERHFNLQSVASMCNLDT", "aa_hexdigest": "1994a274baad84226fc2088274757087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5254.1116, "isoelectric_point": 4.74869327545166 }, "id": "EFPKPENHBP_1526", "locus": "GCA000186005_01515" }, { "type": "cds", "sequence": "contig_1", "start": 837894, "stop": 838478, "strand": "+", "frame": 3, "gene": "traD", "product": "TraM recognition site of TraD and TraG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1", "UniRef:UniRef90_A0AB32XBH1" ], "nt": "ATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAACTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGA", "aa": "MLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNES", "aa_hexdigest": "a58b5514450262ccad7d519232569fc7", "start_type": "ATG", "rbs_motif": null, "genes": [ "traD" ], "truncated": "both", "pseudogene": { "start": 837612, "stop": 838781, "observations": { "mutation": [ 837657, 838476, 838716 ], "stop": [ 837657, 838476, 838716 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBH1", "query_cov": 1.0, "subject_cov": 0.470873786407767, "identity": 1.0, "score": 343.0, "evalue": 1.9e-115, "gene_start": 1, "gene_end": 194, "reference_start": 95, "reference_end": 288, "reference_sequence": "MSICYINAETNKRYYWAELFKNVKTIANIVPETLTGNLSHTTSALKNFTEDEFIKRLTRETKSFDLHGIVSSNEPYVIFIHYPDHKPSNHFLVSMLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNESWNYQDLPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALAWQKILSFQQKYKNKLSSELVNDFTKSINICLDQMNDIKTAGAKT" }, "paralog": false, "identity": 0.9897172236503856, "score": 728.0, "evalue": 1.37e-269, "description": "Internal stop codon at 837657, 838476, 838716. Nonsense mutation around 837657, 838476, 838716.", "psc": { "uniref90_id": "UniRef90_A0AB32XBH1", "product": "TraM recognition site of TraD and TraG", "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBH1", "UniRef:UniRef50_A0AB32XBH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1" ] } }, "id": "EFPKPENHBP_1527", "locus": "GCA000186005_01516" }, { "type": "cds", "sequence": "contig_1", "start": 838491, "stop": 838718, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGA", "aa": "MPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALA", "aa_hexdigest": "a7bce82dcd65675848f6e9422130e73d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8724.933399999994, "isoelectric_point": 9.221702766418456 }, "id": "EFPKPENHBP_1528", "locus": "GCA000186005_01517" }, { "type": "cds", "sequence": "contig_1", "start": 838861, "stop": 839049, "strand": "+", "frame": 1, "gene": null, "product": "ICEF-IA ORF6", "db_xrefs": [ "RefSeq:WP_013526825.1", "SO:0001217", "UniParc:UPI00000C073C", "UniRef:UniRef100_Q8G8G5", "UniRef:UniRef50_Q8G8G5", "UniRef:UniRef90_Q8G8G5" ], "nt": "ATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAA", "aa": "MKRYFYRIRVKKNLESKKTKSYAVCSNFNEADKIANEIIEKGDYGWVQVERVEKIKHYFKEV", "aa_hexdigest": "536d7d5d695db8bc2e435e625cfadd0d", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00000C073C", "ncbi_nrp_id": "WP_013526825.1", "uniref100_id": "UniRef100_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C073C", "RefSeq:WP_013526825.1", "UniRef:UniRef100_Q8G8G5" ] }, "ips": { "uniref100_id": "UniRef100_Q8G8G5", "uniref90_id": "UniRef90_Q8G8G5" }, "psc": { "uniref90_id": "UniRef90_Q8G8G5", "product": "ICEF-IA ORF6", "uniref50_id": "UniRef50_Q8G8G5" }, "pscc": { "uniref50_id": "UniRef50_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G8G5" ], "product": "ICEF-IA ORF6" }, "genes": [], "id": "EFPKPENHBP_1529", "locus": "GCA000186005_01518" }, { "type": "cds", "sequence": "contig_1", "start": 839051, "stop": 839998, "strand": "+", "frame": 2, "gene": null, "product": "MORN repeat protein", "db_xrefs": [ "RefSeq:WP_013526824.1", "SO:0001217", "UniParc:UPI00000C02A4", "UniRef:UniRef100_C4XF99", "UniRef:UniRef50_C4XF99", "UniRef:UniRef90_C4XF99" ], "nt": "ATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCTAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MQVFNALENEEIINRRNYEKNGTNFELISTKSGLTLAHNVLNDNSQEYYEMNEGAFSGRYYKIAENNQLVGVANYQNDELNGEATIYNTKGDILYKAHFEDGLLNGAVEEYKDGALVKSTMYENGFKTGEEVVYDAKGEVEGKTNFITNNEVKEKEVKEYKEADKEKKAEIRNKNFKEVLKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "0cdba05f663e8efc85d8182828c25163", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A4", "ncbi_nrp_id": "WP_013526824.1", "uniref100_id": "UniRef100_C4XF99", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A4", "RefSeq:WP_013526824.1", "UniRef:UniRef100_C4XF99" ] }, "ips": { "uniref100_id": "UniRef100_C4XF99", "uniref90_id": "UniRef90_C4XF99" }, "psc": { "uniref90_id": "UniRef90_C4XF99", "product": "MORN repeat protein", "uniref50_id": "UniRef50_C4XF99" }, "pscc": { "uniref50_id": "UniRef50_C4XF99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF99" ], "product": "MORN repeat protein" }, "genes": [], "id": "EFPKPENHBP_1530", "locus": "GCA000186005_01519" }, { "type": "cds", "sequence": "contig_1", "start": 840007, "stop": 840246, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526869.1", "SO:0001217", "UniParc:UPI00000C02C2", "UniRef:UniRef100_C4XFA0", "UniRef:UniRef50_C4XFA0", "UniRef:UniRef90_C4XFA0" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEEFKKMEPEKYEKFRKESNKKICNKLFAK", "aa_hexdigest": "9df3ce0167d8cced414b46ed0b0c03a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02C2", "ncbi_nrp_id": "WP_013526869.1", "uniref100_id": "UniRef100_C4XFA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C2", "RefSeq:WP_013526869.1", "UniRef:UniRef100_C4XFA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA0", "uniref90_id": "UniRef90_C4XFA0" }, "psc": { "uniref90_id": "UniRef90_C4XFA0", "uniref50_id": "UniRef50_C4XFA0" }, "pscc": { "uniref50_id": "UniRef50_C4XFA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9481.049399999993, "isoelectric_point": 9.631012535095213 }, "id": "EFPKPENHBP_1531", "locus": "GCA000186005_01520" }, { "type": "cds", "sequence": "contig_1", "start": 840259, "stop": 840975, "strand": "+", "frame": 1, "gene": null, "product": "Mbov_0392 family ICE element protein", "db_xrefs": [ "RefSeq:WP_013526868.1", "SO:0001217", "UniParc:UPI00000C0686", "UniRef:UniRef100_C4XFA1", "UniRef:UniRef50_C4XFA1", "UniRef:UniRef90_C4XFA1" ], "nt": "ATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAA", "aa": "MIEKVFCFKDLYYLTQYALSDISDEEVVAKFNYLAKNIKNNTMRDKYKKQVLSIVDKMSNVDDDINQKIFDKLFYVEDTNEKNLEQYITLLNICNVEDLENIENNSLDLKFSFNDFFGENGYIESEYLFDLVDEDLKQNGINSKYIYSLGDLSRIDTEYVYIDDYLNFHPEDIDDIKKEYIENLASLKLDEIIDLNNKESIDANNKVEITDKTVKEAQKHVAGIVEKESETAKVKNTK", "aa_hexdigest": "7a9c47a621909a378797adedb1d43586", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C0686", "ncbi_nrp_id": "WP_013526868.1", "uniref100_id": "UniRef100_C4XFA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0686", "RefSeq:WP_013526868.1", "UniRef:UniRef100_C4XFA1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA1", "uniref90_id": "UniRef90_C4XFA1" }, "psc": { "uniref90_id": "UniRef90_C4XFA1", "product": "Mbov_0392 family ICE element protein", "uniref50_id": "UniRef50_C4XFA1" }, "pscc": { "uniref50_id": "UniRef50_C4XFA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA1" ] }, "genes": [], "id": "EFPKPENHBP_1532", "locus": "GCA000186005_01521" }, { "type": "cds", "sequence": "contig_1", "start": 840985, "stop": 841536, "strand": "+", "frame": 1, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "RefSeq:WP_013526867.1", "SO:0001217", "UniParc:UPI00000C02C3", "UniRef:UniRef100_C4XFA2", "UniRef:UniRef50_C4XFA2", "UniRef:UniRef90_C4XFA2" ], "nt": "ATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAA", "aa": "MNKVILTGRVVSEDYFKAKTTSGKAVLGFVIAVNNEYGGEPDFLNITAFGLQASNFEKYNKKGDLVEVIGKIKRNAYVDRDNHKQYKMEIVADNIIYSAKKVDRNAEGNDEDIIYKDETKLPKEEKEKVVKARLKKKDEMMEPFKQYIGMDPLCQSVYDKYEEEAFNKDVEVKDETKKHGQTL", "aa_hexdigest": "7876c7aa53c01bbcd9e8e8b7b6e1a631", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02C3", "ncbi_nrp_id": "WP_013526867.1", "uniref100_id": "UniRef100_C4XFA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C3", "RefSeq:WP_013526867.1", "UniRef:UniRef100_C4XFA2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA2", "uniref90_id": "UniRef90_C4XFA2" }, "psc": { "uniref90_id": "UniRef90_C4XFA2", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_C4XFA2" }, "pscc": { "uniref50_id": "UniRef50_C4XFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA2" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "EFPKPENHBP_1533", "locus": "GCA000186005_01522" }, { "type": "cds", "sequence": "contig_1", "start": 841536, "stop": 841733, "strand": "+", "frame": 3, "gene": null, "product": "ICEF-IA ORF13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945", "UniRef:UniRef90_Q8G945" ], "nt": "ATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGA", "aa": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVY", "aa_hexdigest": "37a7ed9202ae80cc53604b2722443644", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 841536, "stop": 841790, "observations": { "mutation": [ 841731, 841755 ], "stop": [ 841731, 841755 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8G945", "query_cov": 1.0, "subject_cov": 0.7738095238095238, "identity": 1.0, "score": 122.0, "evalue": 3.68e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVYWVKKRIEYWKQSKKNSDLH" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.29e-53, "description": "Internal stop codon at 841731, 841755. Nonsense mutation around 841731, 841755.", "psc": { "uniref90_id": "UniRef90_Q8G945", "product": "ICEF-IA ORF13", "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8G945", "UniRef:UniRef50_Q8G945" ] }, "pscc": { "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945" ], "product": "ICEF-IA ORF13" } }, "id": "EFPKPENHBP_1534", "locus": "GCA000186005_01523" }, { "type": "cds", "sequence": "contig_1", "start": 841814, "stop": 842119, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MKKSKKFMKFMAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKYDADVYRNDN", "aa_hexdigest": "5ae4a8cbd911d43c80787cf35874157b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11541.700899999993, "isoelectric_point": 5.83141918182373 }, "id": "EFPKPENHBP_1535", "locus": "GCA000186005_01524" }, { "type": "cds", "sequence": "contig_1", "start": 842135, "stop": 842398, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGSQKDVFAYKDSYHGNK", "aa_hexdigest": "ea19c6d5ae4af4eb3676eadbf877fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.355799999998, "isoelectric_point": 4.955473518371581 }, "id": "EFPKPENHBP_1536", "locus": "GCA000186005_01525" }, { "type": "cds", "sequence": "contig_1", "start": 842399, "stop": 842725, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVRKHYHYYFDMKLDNYYEQFINDFALKGTSKTFKDIAIDGLYKQGMWNNYFDLGKDYSKWFYLAFTRSNVEKYHEGFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "46c6c097a53fd654c69c00bd8f34eab2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13261.713499999989, "isoelectric_point": 5.872456932067871 }, "id": "EFPKPENHBP_1537", "locus": "GCA000186005_01526" }, { "type": "cds", "sequence": "contig_1", "start": 842750, "stop": 843115, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEQVYRNYFKEYLDKATNALLDVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDNQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "013ce869a4b1cb22bf3d6b450ba5ea08", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14177.287199999993, "isoelectric_point": 5.539209938049315 }, "id": "EFPKPENHBP_1538", "locus": "GCA000186005_01527" }, { "type": "cds", "sequence": "contig_1", "start": 843119, "stop": 843325, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "EFPKPENHBP_1539", "locus": "GCA000186005_01528" }, { "type": "cds", "sequence": "contig_1", "start": 843497, "stop": 843688, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0395 family pilin-like conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4", "UniRef:UniRef90_C4XFA4" ], "nt": "ATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGA", "aa": "MKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKIL", "aa_hexdigest": "5087b9cbe03a20493b3e281279e3d943", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 843491, "stop": 843778, "observations": { "mutation": [ 843686, 843722 ], "stop": [ 843686, 843722 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA4", "query_cov": 1.0, "subject_cov": 0.6631578947368421, "identity": 1.0, "score": 117.0, "evalue": 4.48e-33, "gene_start": 1, "gene_end": 63, "reference_start": 3, "reference_end": 65, "reference_sequence": "MKMKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKILWAGIVLILVIVGWGIFELVISIQASGGKTS" }, "paralog": false, "identity": 0.968421052631579, "score": 172.0, "evalue": 2.82e-57, "description": "Internal stop codon at 843686, 843722. Nonsense mutation around 843686, 843722.", "psc": { "uniref90_id": "UniRef90_C4XFA4", "product": "Mbov_0395 family pilin-like conjugal transfer protein", "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA4", "UniRef:UniRef50_C4XFA4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4" ] } }, "id": "EFPKPENHBP_1540", "locus": "GCA000186005_01529" }, { "type": "cds", "sequence": "contig_1", "start": 843778, "stop": 844026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGA", "aa": "MSGFFTRVMEIFKYGPTYVLYLLLFFVSLIVFILLAILLSVVKLATFDLLNFVIFGIMPRQNILDHQSPYMYVRFAIVSAVI", "aa_hexdigest": "594dbf9addb063641ab0662d22f90068", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9502.483199999995, "isoelectric_point": 9.222605323791505 }, "id": "EFPKPENHBP_1541", "locus": "GCA000186005_01530" }, { "type": "cds", "sequence": "contig_1", "start": 844093, "stop": 844404, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGA", "aa": "MRSALVFSVKSLFILLAFQITILLFNLFVQKIITLMIGEGNDIVVAFINGLHRLFYPPKLAGDVKDILIPATTNPFGFTINLDISFGSFVALAKKTEHLNIGN", "aa_hexdigest": "1f530bd3ff05364bbc363b2280c88bc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11381.43519999998, "isoelectric_point": 9.223765754699706 }, "id": "EFPKPENHBP_1542", "locus": "GCA000186005_01531" }, { "type": "cds", "sequence": "contig_1", "start": 844257, "stop": 844697, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAA", "aa": "MNICVKNRFTAPKKAKSICSPRKNTPTDNIFPTYLSFHILTFLPSSSKLKGKIIGNIKYKKNNGNIFPMASIPPKTSGIAINTAPIIDRNAAVIKMDAQLPMFKCSVFFAKATKLPKLISKLIVKPKGFVVAGIKISLTSPANFGG", "aa_hexdigest": "be119545e9f8a00836c1c4d3d042829e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15852.893799999973, "isoelectric_point": 10.454209327697754 }, "id": "EFPKPENHBP_1543", "locus": "GCA000186005_01532" }, { "type": "cds", "sequence": "contig_1", "start": 845224, "stop": 845427, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGA", "aa": "MFKFKAMPKKYSNVVDDKNVRDSSDLNPYSTLVENDVVKNKSFSNKLFQKSEHSNYFSVFKLGGKNI", "aa_hexdigest": "da513e353c81c917ec7655a69dc05219", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7754.720499999998, "isoelectric_point": 9.601872825622557 }, "id": "EFPKPENHBP_1544", "locus": "GCA000186005_01533" }, { "type": "cds", "sequence": "contig_1", "start": 845449, "stop": 845613, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGATGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGA", "aa": "MVLIENFARILDSFKYKFSIVKLDEQIDYRRNLNYINTIQSKKMLNSGGETKEF", "aa_hexdigest": "7eabf1fafeeaa9103dfce5aa5be32540", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.395699999999, "isoelectric_point": 9.098954963684083 }, "id": "EFPKPENHBP_1545", "locus": "GCA000186005_01534" }, { "type": "cds", "sequence": "contig_1", "start": 845668, "stop": 846015, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGA", "aa": "MIETFYLILNAPTLEELDECYSTFIQKAEDYEVISYEKLSGFELLSFLNKLNHFNKSEEDIETFLNESDIYESEYSLDRLFKYEKVEFKSNSLKIDDKLYSVKCAGNIPIRLNNE", "aa_hexdigest": "7329907af2238aaf3f1e5143e18b2cfa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13622.117199999982, "isoelectric_point": 4.459382820129394 }, "id": "EFPKPENHBP_1546", "locus": "GCA000186005_01535" }, { "type": "cds", "sequence": "contig_1", "start": 846121, "stop": 846534, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGA", "aa": "MDAGNLDNSIINRFGSALEEESIAVMIDQIQADNFKLFDSNIFVLAEAENAEELKQNNLKIKNGFTRSNFGINDLTFRQFEGFLDMSNYPLSKLDREYYQITSINNAIGYPLKNLPLNDGNNLLLGNEVGGNDSPIV", "aa_hexdigest": "60571d7151425553be189582d3abe5bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15326.890099999975, "isoelectric_point": 4.264652442932129 }, "id": "EFPKPENHBP_1547", "locus": "GCA000186005_01536" }, { "type": "cds", "sequence": "contig_1", "start": 846538, "stop": 846984, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGA", "aa": "MFSLTSSRANHNVMILGTSGMGKSTLTKKILTSSLAAGNIAIIIDPQAEYVKWANKMGGQIVDLGSGDGTVINPLQVKSFIAREEDDANVNFNTLINNHLLWLEKFFSFIFDDMNEMKKNILQLEIIKLYQSWGIYRLKEVKELKNDG", "aa_hexdigest": "7bdeadcb03d81e2d1d7547110e406fd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 49, "aa_cov": 0.3310810810810811, "hmm_cov": 1.0, "evalue": 6.643206648327057e-08, "score": 28.243488311767578, "start": 9, "stop": 57 }, { "id": "PF01935.23", "name": "DUF87", "length": 108, "aa_cov": 0.722972972972973, "hmm_cov": 0.7777777777777778, "evalue": 2.353833018396146e-10, "score": 37.21625900268555, "start": 12, "stop": 118 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 76, "aa_cov": 0.5135135135135135, "hmm_cov": 0.881578947368421, "evalue": 2.441558326642471e-08, "score": 29.528656005859375, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 16628.021899999992, "isoelectric_point": 6.732430839538575 }, "id": "EFPKPENHBP_1548", "locus": "GCA000186005_01537" }, { "type": "cds", "sequence": "contig_1", "start": 846991, "stop": 847797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ], "nt": "ATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MDDLIKQMKKFVAPKDHHDKEGYTKKVLDMAELLEGKFGRYGTLGALYNGHTNINIENDLVVFKTSNLIDTEGSVNARIGIMVLISLVNGFIFDNAFNNKKRINEYKEKNKIRLISKKEIQKLTRYCSFCIDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "fd4e47e07cfb58f2a4c9ea07298295fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F2982C0", "query_cov": 0.9776119402985075, "subject_cov": 0.9225352112676056, "identity": 0.622, "score": 325.0, "evalue": 4.81e-109, "valid": false, "uniref50_id": "UniRef50_UPI001F2982C0" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2982C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30615.79740000003, "isoelectric_point": 8.78963565826416 }, "id": "EFPKPENHBP_1549", "locus": "GCA000186005_01538" }, { "type": "cds", "sequence": "contig_1", "start": 848066, "stop": 848461, "strand": "+", "frame": 2, "gene": null, "product": "ICEF Integrative Conjugal Element-II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7", "UniRef:UniRef90_C4XFA7" ], "nt": "TTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAA", "aa": "MTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK", "aa_hexdigest": "1e201de3510ce021ade96f9646eff4b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 847778, "stop": 848461, "observations": { "mutation": [ 847787, 848057 ], "stop": [ 847787, 848057 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA7", "query_cov": 1.0, "subject_cov": 0.5745614035087719, "identity": 0.992, "score": 242.0, "evalue": 2.31e-79, "gene_start": 1, "gene_end": 131, "reference_start": 98, "reference_end": 228, "reference_sequence": "MKTWEISQENKKGDKLYVKSSRASKNGSKCISTRLSEPDYVALYERWVKRIKQSGSTPYVELKDIIRNALIKQEKEEIAKDFKNDLFYALRKVSWASLTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK" }, "paralog": false, "identity": 0.9780701754385965, "score": 429.0, "evalue": 1.42e-155, "description": "Internal stop codon at 847787, 848057. Nonsense mutation around 847787, 848057.", "psc": { "uniref90_id": "UniRef90_C4XFA7", "product": "ICEF Integrative Conjugal Element-II", "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA7", "UniRef:UniRef50_C4XFA7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7" ], "product": "ICEF Integrative Conjugal Element-II" } }, "id": "EFPKPENHBP_1550", "locus": "GCA000186005_01539" }, { "type": "cds", "sequence": "contig_1", "start": 848507, "stop": 848968, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGA", "aa": "MHAEARAKNEEKMNKKKMLKLIPLSVLSIAIPSVLNLSLNTNKKLLGYGKDFKAPEEKHKIDTNSLFSNFTSMEDDEIAKNERLQNRYQVKWSQLCDKGLLYAQDQWVGYHKTGPVKNKITSIVSRPTLGSFEVRYEKAYYTNPSNHNSYGWF", "aa_hexdigest": "547e92dcc2bb3430ecf9e1292e14eeb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17690.019999999993, "isoelectric_point": 9.477900123596193 }, "id": "EFPKPENHBP_1551", "locus": "GCA000186005_01540" }, { "type": "cds", "sequence": "contig_1", "start": 849080, "stop": 849331, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGA", "aa": "MEEHKGASFTNLFAEYKVSQKTLEFLNNLETDKDKRVYSGLTDDKNKMFLWGLHDFDCEIEGMYDVRYYNNKVLNNLEYAKVG", "aa_hexdigest": "9bda0e8721a59f6b7453f6ce3f3a7db8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9826.907499999998, "isoelectric_point": 5.06073932647705 }, "id": "EFPKPENHBP_1552", "locus": "GCA000186005_01541" }, { "type": "cds", "sequence": "contig_1", "start": 849374, "stop": 849724, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTCACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGA", "aa": "MNRFEYGVNKKIDQNHYSIDLLSDTLGDFTTSVDGVFKDNGGKSNIEHLNKIFDKEYEFEYASGIKVKYKYDYKIKNEAVGEKNKIDVYIKVLSVKIDDKEIIDKDLDKILNNIED", "aa_hexdigest": "3b51870aa20a7ab6eaf58d735c44552d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13509.014199999981, "isoelectric_point": 5.077279472351074 }, "id": "EFPKPENHBP_1553", "locus": "GCA000186005_01542" }, { "type": "cds", "sequence": "contig_1", "start": 849851, "stop": 850051, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGA", "aa": "MYKRLSGASLASRLTISTSITNPDGSISQIEAKTKANGGDLKNLIKRDEPERVSNKELEKDFVGKP", "aa_hexdigest": "e3d98ceff45638283a1d2f30053658e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.081999999999, "isoelectric_point": 9.453788948059085 }, "id": "EFPKPENHBP_1554", "locus": "GCA000186005_01543" }, { "type": "cds", "sequence": "contig_1", "start": 850127, "stop": 850402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGA", "aa": "MVNKQKVSVLNRIFEKKLIDERVNVNDASKIPFDKNSGSGNGEGDVAKNEYLVEVYRYKKGSNNSGEPEEKYVVIYEVIGNQFKQNIKYYA", "aa_hexdigest": "57d3445016ece3a9d5ba8fc5ce158842", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10439.608, "isoelectric_point": 8.752115058898926 }, "id": "EFPKPENHBP_1555", "locus": "GCA000186005_01544" }, { "type": "cds", "sequence": "contig_1", "start": 850418, "stop": 850561, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGA", "aa": "MDQKQIISPYMLDSKGKELKDKEGKLIVNKNYDPSIDKETGTKKQIV", "aa_hexdigest": "819168f07f3459a3efeb395e0df65970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5396.195300000002, "isoelectric_point": 8.96376476287842 }, "id": "EFPKPENHBP_1556", "locus": "GCA000186005_01545" }, { "type": "cds", "sequence": "contig_1", "start": 850574, "stop": 851038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGA", "aa": "MFSDELKEQLRFVYPNIKITTDKADLGIYAEANVLGKGALRNLVVDKVLESSSRYYKCLLFRKENDKYVEIGRKEQKLIEIPVNNGTSEYSYMSEEGIWLLFTVNEKSISNVNLVLIDAKSEPKSFFLDSIENKIEKINNQSEDYFEAFLDGGN", "aa_hexdigest": "14f3e53a5efaf9d6cffd2a93f9497e12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17724.8618, "isoelectric_point": 4.860722923278809 }, "id": "EFPKPENHBP_1557", "locus": "GCA000186005_01546" }, { "type": "cds", "sequence": "contig_1", "start": 851120, "stop": 851218, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGA", "aa": "MISLYKNFVDQASKFNVETGNEYENIFKDKIT", "aa_hexdigest": "1f15fa2f5e0a93065b5e0a705db0eb3e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3787.208700000001, "isoelectric_point": 4.93575038909912 }, "id": "EFPKPENHBP_1558", "locus": "GCA000186005_01547" }, { "type": "cds", "sequence": "contig_1", "start": 851243, "stop": 851410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGA", "aa": "MNYGDETNEAIKENYRRQVKEIVLNFVKRNIANSAKLKAIGITENEINGGWVIAN", "aa_hexdigest": "81fb4d5b440c05c797eb70b5ddfcd8b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.0062, "isoelectric_point": 8.06539783477783 }, "id": "EFPKPENHBP_1559", "locus": "GCA000186005_01548" }, { "type": "cds", "sequence": "contig_1", "start": 851513, "stop": 852205, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGA", "aa": "MRGTGKYINHSITFNLKNSAYHVYYPPIDLSSLDIQKHFEIEMGKNISNDSKELKEKIRNKLIGLIEEQFNLYKNKTGIEMLLNKDIKVEGLDNVLDLLVQGNKFKDGIDIKIKGINANLISSTKIHIINDTEAPAFDLSLLNDTLVKNPIVISQKEPEKITKDIINKLNEITIASGIEFKKDYLVSLVNASFYLLGLVADIDKLNEAKYEKFKKVYEETKAKVMAEYNT", "aa_hexdigest": "765f71697b058a00437b3fa17058fe4a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26199.983000000033, "isoelectric_point": 6.923068809509277 }, "id": "EFPKPENHBP_1560", "locus": "GCA000186005_01549" }, { "type": "cds", "sequence": "contig_1", "start": 852623, "stop": 852772, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTTCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAA", "aa": "MNKKGFKAGKSKKQVSIKKFKEIQIYKKDRIKAKPVVLSDEEINKLFAK", "aa_hexdigest": "df2560211445a3f631e2ef9afa6784c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5709.8361, "isoelectric_point": 10.22006015777588 }, "id": "EFPKPENHBP_1561", "locus": "GCA000186005_01550" }, { "type": "cds", "sequence": "contig_1", "start": 853638, "stop": 854087, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_C4XFA9" ], "nt": "TTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGA", "aa": "MLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHND", "aa_hexdigest": "d11fea40fd11324cc7c0bad85465fa78", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 853347, "stop": 854288, "observations": { "mutation": [ 853377, 853581, 854085 ], "stop": [ 853377, 853581, 854085 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA9", "query_cov": 1.0, "subject_cov": 0.476038338658147, "identity": 0.993, "score": 280.0, "evalue": 6.88e-93, "gene_start": 1, "gene_end": 149, "reference_start": 98, "reference_end": 246, "reference_sequence": "MYNFYMSLELWRPLKITNPLLEDIFHLNIDTKFKIHPLIIFENTKNKTYYCIRLQTARKSSSKYNLIIDNSSYQTNEYWKNHESVVVTKDIFIIDKELLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHNDWKDLDKTKDNVNIYSFENIGFLSKSSKSESSYIDPYDNKNIEKAKDKVAKEIPIKESKTDKDKTKLK" }, "paralog": false, "identity": 0.987220447284345, "score": 610.0, "evalue": 1.67e-225, "description": "Internal stop codon at 853377, 853581, 854085. Nonsense mutation around 853377, 853581, 854085.", "psc": { "uniref90_id": "UniRef90_C4XFA9", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA9", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17230.18999999998, "isoelectric_point": 8.557549476623539 }, "id": "EFPKPENHBP_1562", "locus": "GCA000186005_01551" }, { "type": "cds", "sequence": "contig_1", "start": 854504, "stop": 855250, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0", "UniRef:UniRef90_C4XFB0" ], "nt": "ATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGA", "aa": "MYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLE", "aa_hexdigest": "d325320591f32ac6814f3d3679059197", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 854330, "stop": 855502, "observations": { "mutation": [ 854384, 855248, 855335 ], "stop": [ 854384, 855248, 855335 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB0", "query_cov": 1.0, "subject_cov": 0.6358974358974359, "identity": 1.0, "score": 491.0, "evalue": 3.51e-173, "gene_start": 1, "gene_end": 248, "reference_start": 59, "reference_end": 306, "reference_sequence": "MIMEGAVKNKMSKTLADNWNAQAEVFKNSEERKIKYNVERKIERDIFIWGETVRIIYYMYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLEWITKQEKNKTVYANAKKFKKYYKENREYIWNSVLVKNYFGGCAETVVGHDLKRFCSKKFATFSLKTIQLSIAKNMETKANLFFI" }, "paralog": false, "identity": 0.9897435897435898, "score": 768.0, "evalue": 5.17e-285, "description": "Internal stop codon at 854384, 855248, 855335. Nonsense mutation around 854384, 855248, 855335.", "psc": { "uniref90_id": "UniRef90_C4XFB0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB0", "UniRef:UniRef50_C4XFB0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0" ], "product": "ISLre2 family transposase" } }, "id": "EFPKPENHBP_1563", "locus": "GCA000186005_01552" }, { "type": "cds", "sequence": "contig_1", "start": 856082, "stop": 856264, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527068.1", "SO:0001217", "UniParc:UPI0001A8C360", "UniRef:UniRef100_C4XFH5", "UniRef:UniRef50_C4XFH5", "UniRef:UniRef90_C4XFH5" ], "nt": "ATGTTATTAGTTATTTTAAAAGAAAACATCAATGACAAACAAAAATATTTAAAAGAATATTTAATAACATTCTTATTAAGTGATATATTAGAACACTATAATGTCAAAAATGACTATAAAATAGAGGAATTATTAAAATTCTTAGCTTCTAGTTCTTCATCTTTAACTAATACTAAAAAATAA", "aa": "MLLVILKENINDKQKYLKEYLITFLLSDILEHYNVKNDYKIEELLKFLASSSSSLTNTKK", "aa_hexdigest": "af8ef68bb503186a0d73662924daf43a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C360", "ncbi_nrp_id": "WP_013527068.1", "uniref100_id": "UniRef100_C4XFH5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C360", "RefSeq:WP_013527068.1", "UniRef:UniRef100_C4XFH5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFH5", "uniref90_id": "UniRef90_C4XFH5" }, "psc": { "uniref90_id": "UniRef90_C4XFH5", "uniref50_id": "UniRef50_C4XFH5" }, "pscc": { "uniref50_id": "UniRef50_C4XFH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7095.2107, "isoelectric_point": 7.947291755676269 }, "id": "EFPKPENHBP_1564", "locus": "GCA000186005_01553" }, { "type": "cds", "sequence": "contig_1", "start": 856957, "stop": 857157, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTTAATGCACAAAATATATATTTAAGTGCTTTAGAAGCAAGCACTAAAGATAATAGTTATAATGATTTGTTAAATAATCTTAATAAAAAAGACTTAACTTTATTAAGATATTGCATAGAAAATAAAAAAAGTATTATTCTTATTAAAGATTTAGCTTTAGTCTTTGGCGTTACTTCTAGAGCTATATCAAAATGA", "aa": "MALNAQNIYLSALEASTKDNSYNDLLNNLNKKDLTLLRYCIENKKSIILIKDLALVFGVTSRAISK", "aa_hexdigest": "520df7bb3923570945a2a691df50f1fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7402.5457, "isoelectric_point": 9.206681632995604 }, "id": "EFPKPENHBP_1565", "locus": "GCA000186005_01554" }, { "type": "cds", "sequence": "contig_1", "start": 857179, "stop": 857325, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGATGATTATGATTTCAAAGGATTTAATTCTTTTGAAGAATATTATAGTAATAACTTAAATGAATATTATCAAGCATTACAAATGGATTTAAGTCCTTTATTTTATAATGGACGTAATAAACTGCCACATTTAGAAAAATGA", "aa": "MLDDYDFKGFNSFEEYYSNNLNEYYQALQMDLSPLFYNGRNKLPHLEK", "aa_hexdigest": "8860f11e0467944d43d5caa6eeffcec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.4092, "isoelectric_point": 4.499567985534668 }, "id": "EFPKPENHBP_1566", "locus": "GCA000186005_01555" }, { "type": "cds", "sequence": "contig_1", "start": 857692, "stop": 857961, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAATTAGAACTATCATTTAAATATACTGATGAGCTTGTCAATTATCTTCTAAAAATTGAAAAATACAAAAGTTCATTAGAATTTTTGTCTTTACCTACAAGAAGTAAACAAAGAATTCAATTTGAAGCCAAAATTAAACAAACTCATTTTTCAACAAGTATTGAAGGCAATGTTTTAAACATTGAACAAGTTAAAAACGTTATAAACAATAAAACACATCAAACTAGAATTAAAGCTGAACAAGAAGTATTAAATTATTGA", "aa": "MKKELELSFKYTDELVNYLLKIEKYKSSLEFLSLPTRSKQRIQFEAKIKQTHFSTSIEGNVLNIEQVKNVINNKTHQTRIKAEQEVLNY", "aa_hexdigest": "3d82860e9b0c0ebfc8c324f315e3afd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10577.044399999992, "isoelectric_point": 9.362888526916507 }, "id": "EFPKPENHBP_1567", "locus": "GCA000186005_01556" }, { "type": "cds", "sequence": "contig_1", "start": 858762, "stop": 859091, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527071.1", "SO:0001217", "UniParc:UPI0001F59884", "UniRef:UniRef100_A0AB32XCI0", "UniRef:UniRef50_A0AB32XCI0", "UniRef:UniRef90_A0AB32XCI0" ], "nt": "GTGCTTATTGATAAGGATGGTAGTATTTATATCGACATTTTAGGAATTGAAAAATTTCCTACTAAAACATTGACTAGAGAAGAACTTAACGAATATCAACTTTTTGTACACAATTTAGGTGTGACAGAAAGCACAATATCAAAACTTCTTTATACAAAAATCAAATCAACAAATAGTTTTTATAACTTAATAGAATCTAAAATTGAAAAATTAAAAAATCTTTTACTCACTCTAAATTTAGACAAAAATAAATTAGAAAGCGAAATAAAAGATTTTACTCATAACATCTGTAAGATCAATAAAAAAATAGGTGAATTAATTCAATTCTAA", "aa": "MLIDKDGSIYIDILGIEKFPTKTLTREELNEYQLFVHNLGVTESTISKLLYTKIKSTNSFYNLIESKIEKLKNLLLTLNLDKNKLESEIKDFTHNICKINKKIGELIQF", "aa_hexdigest": "4baa41c4450eac7e5c49f59b799df526", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59884", "ncbi_nrp_id": "WP_013527071.1", "uniref100_id": "UniRef100_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59884", "RefSeq:WP_013527071.1", "UniRef:UniRef100_A0AB32XCI0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCI0", "uniref90_id": "UniRef90_A0AB32XCI0" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCI0", "uniref50_id": "UniRef50_A0AB32XCI0" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCI0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.667799999981, "isoelectric_point": 7.791342735290527 }, "id": "EFPKPENHBP_1568", "locus": "GCA000186005_01557" }, { "type": "cds", "sequence": "contig_1", "start": 859437, "stop": 860636, "strand": "-", "frame": 2, "gene": null, "product": "RNA (S1 domain)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "nt": "ATGGCAATAGACCCTGCTTTTGTTAATGGTTGCAAAATTGCAATCTTAGATCCTCAAGGTAAGTTCTTAAAAAAAGCTTTAATTTATCCTAACCCTCCTCAAAATTATGTTGATAAAGCTAAAGAAATAGTTAATAAACTATTAGATTCATACAAAGTTAATATTATTGTTATTGGCAATGGAACAGCTTCAAGAGAAACTGAAGAATTTATTGCTAATTTATTAAAAGAAAGATCAAAAGTAAATCCTAATGAAGATATTTCTTATGCTGTTGTTAGTGAAGTAGGAGCTAGTGTTTATTCAGCTTCTAAAGTTGCTATTGATGAATTTCCAGATTTTAGTGTTGAAGAAAGATCAGCTGTTAATATTGGACGTCGTTTCCAAGATCCTCTTAATGAATTAGTTAAAATTGATCCTAAAGCTATAGGGGTAGGTCAATATCAACATGATGTTAATCAAAAAGATTTATCTAAGGCTTTAGACTTTAAAGTTGATAAAGTAGTTAACATGGTTGGAGTTGACTTAAATAGTGCTACTAAAGAAATCTTATGCTACATTTCAGGTTTAACTAAAACATTAGCTCAAAACATTATTGATTATCGTGAAAAAATCAATAAATTTAAAGAAAGAAAAGAACTATTAAAGGTTAAAGGTTTAGGCGAAAAAGCCTATGAACAAGCGGTTGGCTTCTTAAGAATCCATGATTCTAAAAACTTTTATGACCGCACAGCTATTCACCCTGAATCATATCCTTTAGCAATTAATATTGTTAATGCGCTTCAATTAGATCTAAATGATATAGACTTAAAAACTATTGAACAACAAGATGCTAATAAATTAGCTAAAGACTTTAATTCAAATGAATATGATGTTAAGTTAATATTAGATTCTTTATCTAATCCAACTAAAGATATTCGTGATGACAAAGATGGCTACATTCTTAAAAAAGATGTTCTTAAGTTAGAAGACTTAAAACCAGGTATGATTCTAGATGGTTCAGTGCAAAATATTACAGACTTTGGTATCTTTGTTTATATTGGAATTAAGCAAGCTGCTTTAATTCACATTTCAAATATGAAAAGAAATAAAGATCACTACATCAAACACTCTAGCGAAGTAGTTAAAACAGGAGATAACATTAAAATCCAAATCCTTGAAATAGATAAAGAAAGAGAAAGAATTCAAGGCAAATTAATTTGA", "aa": "MAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDANKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLI", "aa_hexdigest": "26d8279fcec8ca17602fadcc6d320034", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PA84", "query_cov": 1.0, "subject_cov": 0.9322429906542056, "identity": 0.541, "score": 412.0, "evalue": 3.05e-139, "valid": false, "product": "RNA (S1 domain)-binding protein", "uniref50_id": "UniRef50_A0A3B0PA84", "kegg_orthology_id": "K06959" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PA84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "product": "RNA (S1 domain)-binding protein" }, "genes": [], "id": "EFPKPENHBP_1569", "locus": "GCA000186005_01558" }, { "type": "cds", "sequence": "contig_1", "start": 860661, "stop": 861584, "strand": "-", "frame": 2, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "KEGG:K06959", "SO:0001217", "UniRef:UniRef50_L0RUW1", "UniRef:UniRef90_C4XFG9" ], "nt": "ATGGATTTATCTAAAAAATTTGTAGCACAAAAATTAAACTTAAAAGAAGACCAAGTTGAAGTTGTTCTTAAGTTAATGGATGAAGGAGCAACAGTACCTTTTATTGCCCGTTATCGTAAAAATGTAACTGGTGGACTTGATGAAGAAGTTATTCAACAAATAAGTGACATGTATGTTTACAATGTTGAATTAAACAAAAGAAAAGAAGCTATTATTGACATCTTAAAAGAAAAAGGATTACTCACACCTGAAATTGAAAATAAATTAAAAGCAGCTGAAACTAAAGCTGAAGTTGAAAACATTTATGAACCATTTAAAGTTGGTAAAAAAACTAAAGCTAGTGAAGCTATTGCTTTAGGTTTAGAACCTTTAGCTAAAGCAATTATGGAAGCAACTGATGAAAGTTTTGATCCTTATAAAGAAGCTAAAAAATACTTAAATGACAAAGTACCAACAGTTGAATTTGCTATTGAGCAAGCTCAATACATTATTAGCCAAATTATGTCTCAAGATGTTGAAAATAGAGAATATGTTAAAAAACAAATTTATGATTTTGGTTTCATTGTAACAAAAGTTAAAAAGAATGCTGAAGATGAAAAAGAAGTATTTCAACAATACTATGATTATAAAGAAAGAATCAAGTTTATTCCTAACCATCGTGTTTTAGCAATTAGTCGTGGTGAAGACAAAAAGATTTTAAGTTATGACATGACTTATAATTTAGCTAAAATTACTTATGACTTAAACAACAAATATTTTAAAATCAAAAGAACAGGCAAGATTTTGAACGAATCATTAAAAGACAGTTTAGATCGTTTGATTTTACCTTCAATTATTAGAGAAATCAAAAGTGATCTTTTTGCTCGTGCAGAAAAAGAAGCAATTAACCTTTTCGCAACTAATCTTGAAACAATGTTACTATGA", "aa": "MDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLL", "aa_hexdigest": "f3778c4d57722586d524453307c6b7d7", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 860358, "stop": 861596, "observations": { "mutation": [ 860663 ], "stop": [ 860663 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG9", "query_cov": 1.0, "subject_cov": 0.425207756232687, "identity": 1.0, "score": 578.0, "evalue": 7e-202, "gene_start": 1, "gene_end": 307, "reference_start": 5, "reference_end": 311, "reference_sequence": "MEENMDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLLWPAVKNKTVMAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDANKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLIWED" }, "paralog": false, "identity": 0.9951456310679612, "score": 795.0, "evalue": 2.3e-289, "description": "Internal stop codon at 860663. Nonsense mutation around 860663.", "psc": { "uniref90_id": "UniRef90_C4XFG9", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_L0RUW1", "kegg_orthology_id": "K06959", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG9", "UniRef:UniRef50_L0RUW1", "KEGG:K06959" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUW1" ], "product": "S1 motif domain-containing protein" } }, "id": "EFPKPENHBP_1570", "locus": "GCA000186005_01559" }, { "type": "cds", "sequence": "contig_1", "start": 861889, "stop": 862722, "strand": "+", "frame": 1, "gene": null, "product": "CTP synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "nt": "GTGACTAAATTTATTGTGACAACTGGTGGAGTTTTATCTGGTTTAGGTAAGGGTGTTACCAGTGCAAGTATTGGAAATTTATTAAAGGCACAAGGCTTTAAAGTCTTTGCTTTAAAACTTGATCCATATTTAAATATTGACCCAGGAGTAATGAGTCCCATTGAACATGGTGAAGTATTTGTAACTGCTGATGGTGGTGAGACTGACTTAGACTTAGGACACTATGAAAGATTTATTGATGTTAAGCTAAGCAAGGAAAGTAACTACACAAGTGGAAGAATTTTTACTCGCATTTTTGAAAAAGAACGTCAAGGACTTTATGGTGGAAAAACTGTTCAAATAGTACCTCATGTTATTGATGAAATTATTAGCATTATCGAAAATACTGCTAAAAAACAAAAACCAGATTTCATGTTAATTGAAATCGGAGGAACTGTTGGAGACATTGAAAGTAATCCTTATATTTATGCAATTAGTAAGTTTGCTAGTTTAAATCCTAAAAGTGTTTTCTTTGCTCACTTAGCATTTATTCCTTATTTAACTGCTTCAAAAGAATACAAATCAAAACCATCTCAAGTTTCAATTGCTTCTTTAAGATCATTTGGAATTAACCCTAATATTTTACTTTTAAGAAGTCAAGGCGAAGTTGATGAAAGAATTATTAATAAAGTTGCTGAAGCTTCATTTTTGGACCCTAATCATGTAATTGGAGTTCCTGATAAAAGCAGTATCTATGAAATTCCAATGTTCTTATATGAACAAAATATTTTAAAAATAATTTACTCACATTTTGGAATTAAAAAACAAATTAATGACAAGGTTTATGATTCATGA", "aa": "MTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFGIKKQINDKVYDS", "aa_hexdigest": "2c924df9d86857f0914b074129c2acee", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387385AD", "query_cov": 0.9855595667870036, "subject_cov": 0.8694267515923567, "identity": 0.6809999999999999, "score": 385.0, "evalue": 2.63e-132, "valid": false, "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_A0A223MAG4", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "product": "CTP synthase" }, "genes": [], "id": "EFPKPENHBP_1571", "locus": "GCA000186005_01560" }, { "type": "cds", "sequence": "contig_1", "start": 862732, "stop": 863499, "strand": "+", "frame": 1, "gene": null, "product": "CTP synthase", "db_xrefs": [ "EC:6.3.4.2", "GO:0003883", "GO:0006221", "GO:0006241", "KEGG:K01937", "SO:0001217", "UniRef:UniRef50_Q98PQ3", "UniRef:UniRef90_C4XFG8" ], "nt": "TTGAATAAATATCACGCAACTAAAAATTATAAAATTAACTTACTTTTAGTTGGTAAATATACTGAATTAGAAGATGCTTATTTATCAATTATTTCTTCATTAAAAATAGCTGCTGTTCATCAAAATGTCGAATTAAAATATGACTTAATTGATGCTGATTTAGTTAACAATAAAAACATTGAAAAATTAACAAAAGCACATGATGCAGTAATGATTTTACCAGGCTTTGGTGCTAGAGGTTTTGAATCTAAAGTTAATGTTGCGACTTACACAAGAGAACACAAAATTCCTACTTTAGGTATTTGTTTAGGTTTTCAAGCAATGTCAGTAGCTCAAGCTCGATTATTAGGCATTAAAGATGCAACCAGCAAAGAATTCGCTGAAAATGGTAAAAAACAAACTTTTGTTTTAACTCCTTTTTATGAAAATGGAGACAAAATGGCTATTGGTGGCACACTAAGACTTGGTGAAGATCAAGTTCAAGCTAAAAATAACACCTTAGCTCAAAAAATTTATGGTTCAAATATTTTCTATGAACGTCACAGACATAGATATGAAATTAATCCTGAATACTTAGAAAAATTACAAGATGATGAATTTACATTCAGTGCAATTCATCCAACTAAAAAAGTGGCTGAAATTTGTGAAGTTAAAAACCATCCTTTCTATTTAGGTGTTCAATATCACCCAGAATTTAGTACTAGAGTTTTAGCTTCAAATCCACTTTTTGATAATTTCTTCCAAGCAATTATTAAAAATCAAAAATAG", "aa": "MNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVATYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK", "aa_hexdigest": "dce53efa9165266ca3dd04c6823f5906", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 862432, "stop": 863499, "observations": { "mutation": [ 862720 ], "stop": [ 862720 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG8", "query_cov": 1.0, "subject_cov": 0.46703296703296704, "identity": 0.996, "score": 512.0, "evalue": 4.45e-179, "gene_start": 1, "gene_end": 255, "reference_start": 292, "reference_end": 546, "reference_sequence": "MNPIVLRRIIVTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFGIKKQINDKVYDSWKVFLNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVATYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK" }, "paralog": false, "identity": 0.9971830985915493, "score": 703.0, "evalue": 8.32e-257, "description": "Internal stop codon at 862720. Nonsense mutation around 862720.", "psc": { "uniref90_id": "UniRef90_C4XFG8", "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_Q98PQ3", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG8", "EC:6.3.4.2", "UniRef:UniRef50_Q98PQ3", "KEGG:K01937", "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PQ3" ], "product": "CTP synthase" } }, "id": "EFPKPENHBP_1572", "locus": "GCA000186005_01561" }, { "type": "cds", "sequence": "contig_1", "start": 863511, "stop": 863609, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATTACAAAAAAACATTAAATATGCCTCAAACTAATTTCGATATGCGTGCTAACTTAACAACTAAAGAGCCAGCATTCCGAAAAGAATGA", "aa": "MAIDYKKTLNMPQTNFDMRANLTTKEPAFRKE", "aa_hexdigest": "77547607ab02993b7910716ce7b0018c", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3804.3769999999995, "isoelectric_point": 9.397443580627442 }, "id": "EFPKPENHBP_1573", "locus": "GCA000186005_01562" }, { "type": "cds", "sequence": "contig_1", "start": 863829, "stop": 864074, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGCTTGAGGAAGCACACTTAAAGAAAAATGAATTAAGTGTTGTTGACTTACGTAAAAAAGCTGCTCTTTATGCTCAAGATCAAGTTAATAAACAAGCTGAACAATTCAAAAGCATGCAACTTTTATGTGACTTTAAAAAATACTACATTACTAAAGATCCTAAATTCGAAGCTCAACAATTAAGACTTTTTAAAAAGATGATTTTTAGTGGTCTTATTTACAAAGGATTAAAACCTGTTTATTGA", "aa": "MLEEAHLKKNELSVVDLRKKAALYAQDQVNKQAEQFKSMQLLCDFKKYYITKDPKFEAQQLRLFKKMIFSGLIYKGLKPVY", "aa_hexdigest": "7ce3774468e2a5fef7a2aaf7e5f48aaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 74, "aa_cov": 0.9135802469135802, "hmm_cov": 1.0, "evalue": 2.5187549395561568e-11, "score": 39.24574279785156, "start": 8, "stop": 81 } ], "seq_stats": { "molecular_weight": 9619.299899999995, "isoelectric_point": 9.576665687561036 }, "id": "EFPKPENHBP_1574", "locus": "GCA000186005_01563" }, { "type": "cds", "sequence": "contig_1", "start": 864405, "stop": 864713, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ], "nt": "ATGCAATATGTAAGCCCACTTAATAAAAATGTATGTCCTGTAGTTATAGGCCACCACGTTACTTTAGAAAGTGGTAGTGGTTTAGTTCATATTGCTCCTCTTTTTGGTGAAGATGACTTCCAAATTGGAATCAAAAACTCACTTAAAATGATTATGCATATTGAAGATGATGGAAAAATTAATAAAGAAGGTGGAGCTTACCAAGGTCAATTTTATGAAAAAGCTAATGCTTTAATTATTGAAGAATTAACTAAATCTAAAAACTTACTTTTAGAAAGCTCAATAACTCACTCATATCCACATGACTGA", "aa": "MQYVSPLNKNVCPVVIGHHVTLESGSGLVHIAPLFGEDDFQIGIKNSLKMIMHIEDDGKINKEGGAYQGQFYEKANALIIEELTKSKNLLLESSITHSYPHD", "aa_hexdigest": "18fec76ff2f8107dfec1b0fcc070f139", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9R0", "query_cov": 0.9901960784313726, "subject_cov": 0.9099099099099099, "identity": 0.545, "score": 114.0, "evalue": 8.22e-31, "valid": false, "uniref50_id": "UniRef50_A0A432F9R0" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11306.75819999999, "isoelectric_point": 5.5876367568969725 }, "id": "EFPKPENHBP_1575", "locus": "GCA000186005_01564" }, { "type": "cds", "sequence": "contig_1", "start": 865217, "stop": 865414, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTATCACTAATACTTACGTCATTTGAGTATTCAGAATTGGCTACTCAAAGACGTAAAATATCAGCTCCATTTTTGCTAATTATTTTAAGAGGATCAACAACATTACCTTTTGATTTAGACATTTTTTCACCTTTAGCATCTAAAACAAAACCGTGTGAAACAATGTTTTTGTATGGTGCTTTACCATTATAA", "aa": "MILSLILTSFEYSELATQRRKISAPFLLIILRGSTTLPFDLDIFSPLASKTKPCETMFLYGALPL", "aa_hexdigest": "1fda9c1fed9763886097d352c8bbd79a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7294.634699999999, "isoelectric_point": 7.899971961975098 }, "id": "EFPKPENHBP_1576", "locus": "GCA000186005_01565" }, { "type": "cds", "sequence": "contig_1", "start": 865464, "stop": 866105, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "ATGCTCGGTAATTTAAATGGTTTTAAATATGACTCAAATCTCAAAAGAATTGGCGTACATGCCTATATTAAAGAGCAATTGGAAAAAGTTAAAGCTCAAGTTATTGAAGCTTATGATGAATATAAATTCATTAACGTTATTAAACTTTTAAATAATTATGTAGTTGAATTATCAAGCTTTTATTTAAACATTTCAAAAGATGTTTTATATGTCGAAGCTAAAGATTCAATTAACCGTCTTATGACTTTAACAAATATTTATGAAATTGTTGATTTTCTAATTATTGCTCTTGCTCCAATTCTTCCAACTACAGCTGAAGATGCTTACAAAAACTTTAATAAAGTAAATAAAGCTGAAAGTGTTCATCTCGAAACTTTAGCTAACCCACTCAAAGTTAACGAACAAGTTTTAACTCAATGGCAAGACTTTTTTAAACTTCGTGATGAAGTAAACTTATCACTTGAAAATGCAATTAAAGAAGGTTTAATTAAAAGAACTAATGAAGCTAAAGTTGTAATTAAAAGCGATAGCGACTTTATTAAAAGTCTTGATTTAAGACAATTATTAATGGTTGGTAAAGTTGAATTTGGAGCTCAAACTTCTGTTTCAGTTTTCAAATCAATTAAATGCCAAAGATGTTGA", "aa": "MLGNLNGFKYDSNLKRIGVHAYIKEQLEKVKAQVIEAYDEYKFINVIKLLNNYVVELSSFYLNISKDVLYVEAKDSINRLMTLTNIYEIVDFLIIALAPILPTTAEDAYKNFNKVNKAESVHLETLANPLKVNEQVLTQWQDFFKLRDEVNLSLENAIKEGLIKRTNEAKVVIKSDSDFIKSLDLRQLLMVGKVEFGAQTSVSVFKSIKCQRC", "aa_hexdigest": "7d34ec40ca24057487bbf102d1303a56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 154, "aa_cov": 0.7136150234741784, "hmm_cov": 0.948051948051948, "evalue": 2.858846932175577e-23, "score": 78.73269653320312, "start": 22, "stop": 173 } ], "seq_stats": { "molecular_weight": 24419.04140000001, "isoelectric_point": 7.664419746398925 }, "id": "EFPKPENHBP_1577", "locus": "GCA000186005_01566" }, { "type": "cds", "sequence": "contig_1", "start": 866767, "stop": 866904, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTAGATAAAAGAAGAGAAGATACACACCAAGAAGCACAAGAAATAATAGATGCAATTCAAGAAGACAATAGTCAAAATAATACTGTTGAAAATGATCCAAAAGAAGAACAAGAAGACAATAAAAAAGAATAA", "aa": "MILDKRREDTHQEAQEIIDAIQEDNSQNNTVENDPKEEQEDNKKE", "aa_hexdigest": "81dcc8efedbbd55ba062378f701c432f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5368.595700000001, "isoelectric_point": 4.316603279113769 }, "id": "EFPKPENHBP_1578", "locus": "GCA000186005_01567" }, { "type": "cds", "sequence": "contig_1", "start": 867029, "stop": 867847, "strand": "+", "frame": 2, "gene": null, "product": "Schlafen AlbA-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5", "UniRef:UniRef90_C4XFG5" ], "nt": "ATGAAATTTGTTGAAACAAATAAAATCGAATTAAAGGAAAAATTAAATGATAACTTAATTAGAGAAATTGTTGGCTTTTTAAACACAGAAGGCGGAACAATTTATATAGGTGTAAAAGATAATGGTGAAGTAATTGGCATAAAAGATGTAAATAAAGCTTTTACTGAATTAAACGAAATTATTTGTGATCAAATTGAACCAAGAGCGGAAAAAGAAGTAAAAAGTGAAATAAAATTTGAAAATAACAAAATGATTATGGGAATAAACGTTCAAAAAGGAGAAAAAGCTATTTATTACATAAAAAAATATGGTTGTTCTTCAAAAGGATGTTTAGTAAGAGTTGGGACAAGCACAAGAGAAATGTCAAATGAAGAAATAGAAGAAAGAAACAAAATTAGATTTTATGATGAGGACAACATAATAAATTATGTTAGTGATTTGGATGATTTGAAATTTTCAATATTAAAAATAAATTATGAACTAAATAATTTACATTTAGATGATAAAACATTTGAAAAAAATTTACATTTAAGAAATAAATTAGGTAGTTACAATAAGCTAGCTGAATTGCTAGCTAATGAAAATAAAATTGACTTGAAATATGTAAAATTTCAAGGAAATGATAAAACAGTCTTTTCAGAAATACAAAAATTCAGTAATAAAAGTATATTGGTTGGTTATTCACAAATTTTAGCAAGATTAGAAGTTGAAAATAAATGCTATGTAGATACTAGTTCAATGCAAAGAATTGAAAAGACTTTATTTGATTTTAATGTAGCTAAAGAGGCTTTATTAAATGCAATTGTACATAATGATTGA", "aa": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFTELNEIICDQIEPRAEKEVKSEIKFENNKMIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLDDKTFEKNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHND", "aa_hexdigest": "e3049b3d076661a01ec2011e18d3155c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 867029, "stop": 868150, "observations": { "mutation": [ 867845 ], "stop": [ 867845 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG5", "query_cov": 1.0, "subject_cov": 0.6126126126126126, "identity": 1.0, "score": 525.0, "evalue": 1.26e-185, "gene_start": 1, "gene_end": 272, "reference_start": 1, "reference_end": 272, "reference_sequence": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFTELNEIICDQIEPRAEKEVKSEIKFENNKMIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLDDKTFEKNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHNDWLSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGKWSVLGIKPEN" }, "paralog": false, "identity": 0.9973190348525469, "score": 735.0, "evalue": 8.96e-271, "description": "Internal stop codon at 867845. Nonsense mutation around 867845.", "psc": { "uniref90_id": "UniRef90_C4XFG5", "product": "Schlafen AlbA-2 domain-containing protein", "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG5", "UniRef:UniRef50_C4XFG5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5" ], "product": "Schlafen AlbA-2 domain-containing protein" } }, "id": "EFPKPENHBP_1579", "locus": "GCA000186005_01568" }, { "type": "cds", "sequence": "contig_1", "start": 867848, "stop": 868333, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13749.12", "PFAM:PF08279.18", "PFAM:PF08220.18" ], "nt": "TTGAGTGGCGTACCAACTATCCAACAATTTGAAAATAGAATTGAAATTATGTCTTATGGTGGATTACCAAAAGGTTTGAAACTTGAAGAATTTTATCAAGGAATAAGTAAGCCAAGAAATCCAAAATTAATGAAAATATTTATAGATCTTAATTTGGCAGAACAAACCGGACATGGCGTTCCACTTATTCTAAAAAAATATGGAAAAATGGCAATTGAAGTAGAAAAAAACTATATCAGGATAACAATACCTTTTAACAAGGAATTGCTACAAAAAAATAACAATTATGAAATTAAAGAAATAAAAGACAAATTAAACAAAATCGAAAATCAAATATTCGAATTAATTCAAAAAAATGAAGAATATACAGCTTATGAATTGTCAAACGAATTAGAAGTTTCTAAAAGAACAATAGAAAGAGCTTTAGCATCTTTAAAAAACAAACTAATTTTAAAAAGAATTGGTTCAAGAAAAACAGGAAAATGA", "aa": "MSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGK", "aa_hexdigest": "664f051e93aff4d5ed5fb5b280f5b65a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13749.12", "name": "HATPase_c_4", "length": 66, "aa_cov": 0.39751552795031053, "hmm_cov": 1.0, "evalue": 2.4659116629184812e-15, "score": 52.635982513427734, "start": 6, "stop": 69 }, { "id": "PF08279.18", "name": "HTH_11", "length": 41, "aa_cov": 0.2484472049689441, "hmm_cov": 1.0, "evalue": 4.607571320578678e-09, "score": 32.40493392944336, "start": 110, "stop": 149 }, { "id": "PF08220.18", "name": "HTH_DeoR", "length": 45, "aa_cov": 0.2795031055900621, "hmm_cov": 1.0, "evalue": 1.552342418073901e-06, "score": 24.176462173461914, "start": 110, "stop": 154 } ], "seq_stats": { "molecular_weight": 18713.68890000001, "isoelectric_point": 9.579115486145017 }, "id": "EFPKPENHBP_1580", "locus": "GCA000186005_01569" }, { "type": "cds", "sequence": "contig_1", "start": 868385, "stop": 868648, "strand": "+", "frame": 2, "gene": null, "product": "prolyl aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "nt": "ATGTACAAAAAATATTTATATCCAAAAATTGAACCTTATCAAAAAGGTTATTTAAAAGTAGATGATATTTATCAAATTTATTATGAAGTTAGTGGCAATCCACAAGGTCAACCAATAGTTTATGTTCATGGTGGTCCTGGTGGAGGAACTAGTGAAGTATGTAGAAGATATTTTGACCCAAAATACTATAAAATAGTTTTATTTGACCAAAGAGGTTGCGGAAAAAGTACTCCAAGTTTAGAGTTAAAAAATAATACAACTTGA", "aa": "MYKKYLYPKIEPYQKGYLKVDDIYQIYYEVSGNPQGQPIVYVHGGPGGGTSEVCRRYFDPKYYKIVLFDQRGCGKSTPSLELKNNTT", "aa_hexdigest": "3de01bb3ebf32601049827166c585ef4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0017C9CCE2", "query_cov": 0.9770114942528736, "subject_cov": 0.9042553191489362, "identity": 0.612, "score": 122.0, "evalue": 8.64e-35, "valid": false, "product": "alpha/beta fold hydrolase", "uniref50_id": "UniRef50_A0A7V9FP21" }, "pscc": { "uniref50_id": "UniRef50_A0A7V9FP21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "product": "prolyl aminopeptidase" }, "genes": [], "id": "EFPKPENHBP_1581", "locus": "GCA000186005_01570" }, { "type": "cds", "sequence": "contig_1", "start": 869180, "stop": 869329, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTGTCGCCCAAGTGGTGCTTATGAACTTCATCAAAAACTAAATAATTCGCATTTAATTATGGTTGAAAAAGCAGCTCATTCACAAAGAGAAGTTAATATAACTAAGGCTTTAGTTAAAATTACAGATCAAATTAGAAAAAAATAG", "aa": "MDCRPSGAYELHQKLNNSHLIMVEKAAHSQREVNITKALVKITDQIRKK", "aa_hexdigest": "7ea3557e6a9d014839986bf3c9b253e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5644.516, "isoelectric_point": 9.652931785583494 }, "id": "EFPKPENHBP_1582", "locus": "GCA000186005_01571" }, { "type": "cds", "sequence": "contig_1", "start": 869338, "stop": 869547, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_129622071.1", "SO:0001217", "UniParc:UPI000FF6D7AB", "UniRef:UniRef100_A0AB32XD63", "UniRef:UniRef50_A0AB32XD63", "UniRef:UniRef90_A0AB32XD63" ], "nt": "ATGGAAGTAAAGGGTTATACATATTTAGCTACATCTATGATGTGGCTTTTTTATTCTAAATTTAAATCAGATGCTTTTACTCCATTTTTACTTTTCATTTTTTCTAAAATAGTTTTAAGTTTATTTAATAATAATTCAATTCTTAATTTGTCTTATGGAAAAGATTATTTTATAGAAAATATAGATAATCCAAAATTTCTTAAAATATAA", "aa": "MEVKGYTYLATSMMWLFYSKFKSDAFTPFLLFIFSKIVLSLFNNNSILNLSYGKDYFIENIDNPKFLKI", "aa_hexdigest": "2a3110a198dad2a49e4108e22362e97d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000FF6D7AB", "ncbi_nrp_id": "WP_129622071.1", "uniref100_id": "UniRef100_A0AB32XD63", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FF6D7AB", "RefSeq:WP_129622071.1", "UniRef:UniRef100_A0AB32XD63" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD63", "uniref90_id": "UniRef90_A0AB32XD63" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD63", "uniref50_id": "UniRef50_A0AB32XD63" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD63" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8182.522899999993, "isoelectric_point": 8.963700294494632 }, "id": "EFPKPENHBP_1583", "locus": "GCA000186005_01572" }, { "type": "cds", "sequence": "contig_1", "start": 869726, "stop": 870211, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527078.1", "SO:0001217", "UniParc:UPI0001F5988B", "UniRef:UniRef100_A0AB32XCW2", "UniRef:UniRef50_C4XFG2", "UniRef:UniRef90_C4XFG2" ], "nt": "GTGAAACTATCGATCAAAGAAATTATCAAGAGAATTAAAATTCTTGAGGACGAATTAATTGTTCTTAATCAAGAAGAAGATAAAGATAATTATGTTATATATTTAAAAGATGAAACAGTTGAAAAATCATCGTATAATTTTAATAAAACTAATAAAAAACGTCAAGAAATTAATCAAGAAATTTTAAGACTTAAAATTATTGTTCAAAAAGCAAACATCAAAACAATAACTAGTTATAAAGGATTAAGTATTTCTGAACTTTTAATTTTATTAGCACAAAAATCTTCTTTAGTTCAAAGATTTGATTTGTTATGTTCTAAAAAGCAAAAAACAAGAAAAACTACCAACGATGGTCAAATTGAATATATTGAATATTTATATGATATTAACGAATTAAAAAATACTAATTTAAAATTAAAACAAGAAATAGCAACAATGCAAGTTGCTATTGATAAAGCTAATATTGAAACATTAATTGAAATATAA", "aa": "MKLSIKEIIKRIKILEDELIVLNQEEDKDNYVIYLKDETVEKSSYNFNKTNKKRQEINQEILRLKIIVQKANIKTITSYKGLSISELLILLAQKSSLVQRFDLLCSKKQKTRKTTNDGQIEYIEYLYDINELKNTNLKLKQEIATMQVAIDKANIETLIEI", "aa_hexdigest": "20c7a4d9986065e034274e9e251fc3fc", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F5988B", "ncbi_nrp_id": "WP_013527078.1", "uniref100_id": "UniRef100_A0AB32XCW2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5988B", "RefSeq:WP_013527078.1", "UniRef:UniRef100_A0AB32XCW2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCW2", "uniref90_id": "UniRef90_C4XFG2" }, "psc": { "uniref90_id": "UniRef90_C4XFG2", "uniref50_id": "UniRef50_C4XFG2" }, "pscc": { "uniref50_id": "UniRef50_C4XFG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18910.837600000003, "isoelectric_point": 8.96382923126221 }, "id": "EFPKPENHBP_1584", "locus": "GCA000186005_01573" }, { "type": "cds", "sequence": "contig_1", "start": 870315, "stop": 870521, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAATCTGCGATAATTTAAGCATGACAACAAATACTAATAAAAATTCTTTAACAACAACCATTCAATTAATATTAGAAGATGGGACATTAGACGGGATTTTGTATCTCAAAACTTTAAGATGGCCTCTAGGAGGAATTATGTTGGTAAGTCCTCGCGATAAGGTTACTAAATTATTGGACGAATATGAAGAATGCAATAATTGA", "aa": "MLICDNLSMTTNTNKNSLTTTIQLILEDGTLDGILYLKTLRWPLGGIMLVSPRDKVTKLLDEYEECNN", "aa_hexdigest": "991e593e6f90afe58f95af4846ab850a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7688.845199999999, "isoelectric_point": 4.563057136535646 }, "id": "EFPKPENHBP_1585", "locus": "GCA000186005_01574" }, { "type": "cds", "sequence": "contig_1", "start": 871080, "stop": 871292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45E34", "UniRef:UniRef90_UPI000FF45E34" ], "nt": "GTGCTAAATGATATAAATTCTCAAGAAATAACAATTTTGCAAATTCCAGCCAAAAAATTTGAAATTTCAGATACTAAAGGAACGAACAAATTTCAAAAAACTAGAAGTGATAATAAATTTGATTTATATATAAATAATGTTTTATTCGAAAAACAAAATAAAATTGACTTTACAGAATATGTAATAGATAAAATTCGTTATTCTTCCTCATAA", "aa": "MLNDINSQEITILQIPAKKFEISDTKGTNKFQKTRSDNKFDLYINNVLFEKQNKIDFTEYVIDKIRYSSS", "aa_hexdigest": "dceb99835d3e0becdfbec2a52cad4861", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF45E34", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9570000000000001, "score": 130.0, "evalue": 2.71e-38, "valid": true, "uniref50_id": "UniRef50_UPI000FF45E34" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF45E34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45E34" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8281.2759, "isoelectric_point": 7.942907905578614 }, "id": "EFPKPENHBP_1586", "locus": "GCA000186005_01575" }, { "type": "cds", "sequence": "contig_1", "start": 871358, "stop": 871588, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1", "UniRef:UniRef90_UPI0001E32FA1" ], "nt": "GTGGTATTTACTAAGGATTTAAATGTTTATGTTCATTTTGCTTTTATTATTTTAGCTATCTTATTCTTAATCTTTGCAACAGTAATTATTAATTATGTTTTCTATAAATATGTTGTTGGTTTACATAAAATATTTAGCGATAAAGATAAAAACAATTCTGATATTCAAAATCAAAAAACAATTGTTGAAGGAATTGAAGTTACTAAAGTTGACTTAGATTCTTTAGAGTAA", "aa": "MVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE", "aa_hexdigest": "76c01552a3dd2aa9b2239952e9e571be", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 871358, "stop": 871831, "observations": { "mutation": [ 871648, 871708 ], "stop": [ 871648, 871708 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FA1", "query_cov": 0.9868421052631579, "subject_cov": 0.47770700636942676, "identity": 1.0, "score": 138.0, "evalue": 2.94e-40, "gene_start": 2, "gene_end": 76, "reference_start": 83, "reference_end": 157, "reference_sequence": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVGWAAYFYSLAISIKSKKFYFTWACFTFPIAISSTALFKYAQVVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE" }, "paralog": false, "identity": 0.9808917197452229, "score": 300.0, "evalue": 1.38e-106, "description": "Internal stop codon at 871648, 871708. Nonsense mutation around 871648, 871708.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FA1", "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FA1", "UniRef:UniRef50_UPI0001E32FA1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8816.216200000003, "isoelectric_point": 5.5337533950805655 }, "id": "EFPKPENHBP_1587", "locus": "GCA000186005_01576" }, { "type": "cds", "sequence": "contig_1", "start": 871706, "stop": 871831, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTAAGTTTTGCATTTTTAACTTTATTTGATCCTAAGAATCCAAACGCAAATATTTTAAATAATTATGTAATTTTATATACTTTTACAGGAATATTATTCTTATTTATTTTAGTTGGTTGA", "aa": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVG", "aa_hexdigest": "f9947ede4f3897ac5b704ebd3b555576", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4668.535199999998, "isoelectric_point": 5.587011528015137 }, "id": "EFPKPENHBP_1588", "locus": "GCA000186005_01577" }, { "type": "cds", "sequence": "contig_1", "start": 872039, "stop": 872521, "strand": "-", "frame": 3, "gene": null, "product": "C4-dicarboxylate transporter/malic acid transport protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG0", "UniRef:UniRef90_C4XFG0" ], "nt": "ATGACTTTTAAAAATAAATTAGAAAAAGTACCTCTTGGTTTAGGAGGACTTGCACTTGGCATAAGTGGTTTAGCTGGTGCTTATAATGGGGCTTTTAATGAATTTAAAAACTCAAGCAATGGAAAATTAATTAATAATGTTTCAATTGGCATTCAAGCTTTTTGGATGTTAATTGCTTTTGCTTTAGCTTTTATTGTTTGCTTAAGATTTATTAAAGCAAGGCACGTATTTAATAAAGAAGCAAAAGCTCCAAATACAGCAGCATTTATACCCACTTTATTAATGACATTTATTTCTATTTTTACTTTTATAGCCTCAATATTAAATACTTATTTAGAAGCTAGTGAAGCTAAAAAATGGTTATATATTCCATTGGTATTTTCATTCATTGCTTTTATATTACAAATAGCTTATTTAATAACTTTTATAGTATTAATTATAGTTCAACATGATTTTAAATATGATGAAGCTTTTGCTTCTTGA", "aa": "MTFKNKLEKVPLGLGGLALGISGLAGAYNGAFNEFKNSSNGKLINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFTFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVQHDFKYDEAFAS", "aa_hexdigest": "8072d51ff5118b8e2ac614d4681ec806", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 871736, "stop": 872521, "observations": { "mutation": [ 871951, 872041 ], "stop": [ 871951, 872041 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG0", "query_cov": 1.0, "subject_cov": 0.4134366925064599, "identity": 1.0, "score": 303.0, "evalue": 1.27e-100, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MTFKNKLEKVPLGLGGLALGISGLAGAYNGAFNEFKNSSNGKLINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFTFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVQHDFKYDEAFASWFIPTVGFVILTFGANNYQNIIPLLFYQILWFIGLLFCFLIFILVAFKVAFVGHKNHQDIPSLAIFCAPAMVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVGWAAYFYSLAISIKSKKFYFTWACFTFPIEISSTALFKYAQVVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE" }, "paralog": false, "identity": 0.9923371647509579, "score": 509.0, "evalue": 2.11e-184, "description": "Internal stop codon at 871951, 872041. Nonsense mutation around 871951, 872041.", "psc": { "uniref90_id": "UniRef90_C4XFG0", "product": "C4-dicarboxylate transporter/malic acid transport protein", "uniref50_id": "UniRef50_C4XFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG0", "UniRef:UniRef50_C4XFG0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG0" ], "product": "C4-dicarboxylate transporter/malic acid transport protein" } }, "id": "EFPKPENHBP_1589", "locus": "GCA000186005_01578" }, { "type": "cds", "sequence": "contig_1", "start": 872981, "stop": 873391, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATTTAATGTCAATTTGGTCAAAGAATCGAGAATTAGTTTACTAAGAAGATTATCAAAAGTAGTTTATAAAGTTGCTAGAATTAGATTCTTTAACAAATTTATTTCATTTAAAGGTGAATCACTAACAATGCCAATTAAAAAAATAGTTCAAAGTTTAAATGATGACTGTACAAACTTTGATAAATTAGCATACAATAATTTTGTATTTTTAAGAAAATATGCTGAAATTGATGAAATAAATAAAAAGATGGAACAAGATTTGTTAAATAAGAAAACAGATTTATTAGCATCTTCAGACGATCCAGTTTCTTTATTACTTTTAATAAATTATAGTATAGGAAAAAATAATAAAAATCTACACTATTTTGAAAATGATGAACAATCTTCTTATTTTAAAGATTGA", "aa": "MNLFNVNLVKESRISLLRRLSKVVYKVARIRFFNKFISFKGESLTMPIKKIVQSLNDDCTNFDKLAYNNFVFLRKYAEIDEINKKMEQDLLNKKTDLLASSDDPVSLLLLINYSIGKNNKNLHYFENDEQSSYFKD", "aa_hexdigest": "73f666d7ac5f54d826365e1116d9d8af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16030.319399999978, "isoelectric_point": 9.254775047302243 }, "id": "EFPKPENHBP_1590", "locus": "GCA000186005_01579" }, { "type": "cds", "sequence": "contig_1", "start": 873617, "stop": 873889, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAACCATTAACGTCATACGATTTAGTTCGTAATTTTGTTTATGGTCAACTAAGTGAAAAAAGTTCAAATGTAGAATCAAACATTAGCAAATTTGACAATTTCTTTAAACCATTATTTATTAGTAAAAGTGAAAGAAAAACACTTGTCTTGGATTCAAAATTGTTTGATAATTACGTTAACTCAATGGCAACTATTAATAACGTATCAATTAATTCAAATGAATTATCAAACAATTTAACCTACAATATTTTTGTAGAAGTTTGA", "aa": "MYSKPLTSYDLVRNFVYGQLSEKSSNVESNISKFDNFFKPLFISKSERKTLVLDSKLFDNYVNSMATINNVSINSNELSNNLTYNIFVEV", "aa_hexdigest": "7c943b39fd45e745b988763a6e7e5306", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10376.561799999992, "isoelectric_point": 6.161540031433105 }, "id": "EFPKPENHBP_1591", "locus": "GCA000186005_01580" }, { "type": "cds", "sequence": "contig_1", "start": 874115, "stop": 874339, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTAATATTAATTGCATATTCTTTATATAAATTGTTTTTACATGCTAAAAATTTGAAATTATTTTTACCAAAAGATCCTGTCGAAAATATGTTTAGATATTACATTGATATAAAAGACAATTCTAATAGTTTATTGCCTAGAAAAATAACAAGTATTTATAACAATATTAGTGAAAGTGATTCATACAATTCTCTTAGCATCGCTTTAGGTGATAATTGA", "aa": "MTLILIAYSLYKLFLHAKNLKLFLPKDPVENMFRYYIDIKDNSNSLLPRKITSIYNNISESDSYNSLSIALGDN", "aa_hexdigest": "5dd3def6fe32bacddafe3c2f02afe84d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8575.815299999995, "isoelectric_point": 7.963795661926269 }, "id": "EFPKPENHBP_1592", "locus": "GCA000186005_01581" }, { "type": "cds", "sequence": "contig_1", "start": 874496, "stop": 874816, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTATTATAAGGAATTAATAAACAAAATTGAATTAAATAATGAAAACACAAATTTAAATTCTTTTAATGAAAATATGGATGAATTATTAAACATTTTAAACGCTAAAAAAGATGATGATATAAGTAATATTTTATATACAAATGAAGAAGCAATTGTTACAAAAGATTTGGATAGAAATAAATTTTATATTGATGATAATGAAGCAATTTATAAAGAATTAGCTCCTTCTTTTGATGTATTAAATATTCAATCATATTTTAATAAACTTTTAAATATGAAGATATCTATACCAGTATTTCAAAGAAGTTACGTATGA", "aa": "MSYYKELINKIELNNENTNLNSFNENMDELLNILNAKKDDDISNILYTNEEAIVTKDLDRNKFYIDDNEAIYKELAPSFDVLNIQSYFNKLLNMKISIPVFQRSYV", "aa_hexdigest": "9aeba6105ae60bbc1a27f0f1694fcf82", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12539.965599999989, "isoelectric_point": 4.467908668518065 }, "id": "EFPKPENHBP_1593", "locus": "GCA000186005_01582" }, { "type": "cds", "sequence": "contig_1", "start": 874982, "stop": 875203, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTTTAGAAGAATTAAAAATATTAGATAATTTTCATGAAAATTATTACAAATTCGACGAAGATTTTATTATTGAATTAAAAGCTTATTTTGGAAGTAACTTAGAAAACGAATTAAACTATAAAAAAGTAGATTTGGAAAAATTTGTATATAAATATATTTTGCGAAATGTTAACAAAAAATTAAAACAAAATTTAATTAAAAAATTAAATAAATGA", "aa": "MEVLEELKILDNFHENYYKFDEDFIIELKAYFGSNLENELNYKKVDLEKFVYKYILRNVNKKLKQNLIKKLNK", "aa_hexdigest": "6a3ddd0d2dca754a56ee1c1e85ca5449", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9023.364699999993, "isoelectric_point": 7.862902641296388 }, "id": "EFPKPENHBP_1594", "locus": "GCA000186005_01583" }, { "type": "cds", "sequence": "contig_1", "start": 875484, "stop": 875840, "strand": "-", "frame": 2, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080B288" ], "nt": "ATGGCTTTTGAAAATAAAAATTATTCAACTATTTATAAGAATCAAAAGATTGATGAATGTCGTATTAACACATTGACAACCAAACAAGATCGTCATCCTAATGTTGGGGTTTTAGATTATGATGCTAATTTAAAAGGTTTTTATAACAAAAGATTTGTAACAAATAGAGAAGCTTACAAAATTATGGGATTTGAAGATAAAGATTTTGAAAAAGTTAATGAATTTAAGAAAAAAAACATAATAACAAAAGAAGCTCTTTATCGTCAAGCAGGCAATTCAATTTGTGTGCCAGCTATAAAAAGTGTATTTCAGTTAATAGCAAAAATCGAAGAATATAATGGAGGTCAAAATGATTAG", "aa": "MAFENKNYSTIYKNQKIDECRINTLTTKQDRHPNVGVLDYDANLKGFYNKRFVTNREAYKIMGFEDKDFEKVNEFKKKNIITKEALYRQAGNSICVPAIKSVFQLIAKIEEYNGGQND", "aa_hexdigest": "fbec0743b0d70735c4ada3083e8f725b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080B288", "query_cov": 0.9152542372881356, "subject_cov": 0.9908256880733946, "identity": 0.519, "score": 107.0, "evalue": 5.47e-28, "valid": false, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI003080B288" }, "pscc": { "uniref50_id": "UniRef50_UPI003080B288", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080B288" ], "product": "DNA cytosine methyltransferase" }, "genes": [], "id": "EFPKPENHBP_1595", "locus": "GCA000186005_01584" }, { "type": "cds", "sequence": "contig_1", "start": 876237, "stop": 876479, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGACTAGTAAAAAAGCTATTAGTAGCTTAAAAAACAAAAGTTATGAACTTAAAAAAGCCCTAGTAGCTGCAAATCTAATATCAAATAATTTTAATGATATTACAAAGATTACTAAAGAACAATTAGATCAGTTACATTTCGATTTAATAACTTATTCATTCCCTTGCCAAGGTCTTTCAAGTGCTAATATGGGTCGTTCGTTAGGAATTAAAAACAACAATTCAACTAGTCATTTAGTTTGA", "aa": "MTSKKAISSLKNKSYELKKALVAANLISNNFNDITKITKEQLDQLHFDLITYSFPCQGLSSANMGRSLGIKNNNSTSHLV", "aa_hexdigest": "486cbdf74bdb9759525870d8ecd6f476", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 40, "aa_cov": 0.5, "hmm_cov": 0.975, "evalue": 9.08071483610214e-10, "score": 34.73849868774414, "start": 24, "stop": 63 } ], "seq_stats": { "molecular_weight": 8846.046199999995, "isoelectric_point": 9.61212329864502 }, "id": "EFPKPENHBP_1596", "locus": "GCA000186005_01585" }, { "type": "cds", "sequence": "contig_1", "start": 877070, "stop": 878167, "strand": "-", "frame": 3, "gene": null, "product": "Dihydrolipoyl dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "nt": "ATGCATGAACGTAAAAAAGGCGTAGTAGATCAAATTTCAAGTAGTGTTAAAAAATTAATGATTGCTTCTAAATGTAAAATAGAAGAAGGCGAAGCAGAATTTGTTGGAGCTCACGAAATTAAAATAAATGGTAAAGTATATCGTGGCACAAATATTATTTTAGCCACAGGCAGTCACTCACGTAGATTGCGTGCTTTACCTGGATTTAAGGTTGGATATGAAAATAATTATGTTCTTTCAAGCCGCGAAGCAATTAACTATGATTCAAAATTGCCTGGATCGGTAGTTATTGTTGGTGGTGGTGTTGTTGGCGTTGAATTTGCTCAAGTATTTGCTTCAGCAGGTGCTAAAGTAACTATTATTCAAAACCAAAACCACTTACTTCCTGGTATTGATCATGATGTAACAAATGAAATTCTTAAACATCTAGAAAAGCATGGGGTTCAAATTATTTACAATGCAACTTCAACAGGTTTAAATAATAAAAAAGAACTTCTTTACGAAATTGGTGGTAAAGAAAGAAAAATTAAAGCTGATGTTTATTTGATTGCTGTTGGACGTATTCCATCAAGTAAAGGTATTGCAGAAGTTGGAGTTAATGTCGGAGTTAGAGAGGAAGTTCTAGTTGATGAAAAAATGAGAACAAATGTTAAAGGCGTTTATGCAATTGGAGATTTAACTGGACAAAACATGTTAGCTCACGTTGCATATCAACATGCTTTAATTGCTGTTGGTAACATTTTAGGTGAAAAAAATGTTCGTTATCACAATAAACCAGTTCCAGGATGTATTTATACAAACCCTGAAATTGCTTTCATTGGTTTAACTGAAGAAGAAGCAAAAAATAAAGGATACAATATTTTTACTTCAAAATACATGTTCTCTTATTTAGGAAAAGCTATTGCAACCAAACAAACTTCTGGTTTTGTTAAATTAGTTGTTGATCGTGAATATGGGCAAATATTAGGAGCTCACATTATTGGAGCTCATGCAACTGACTATATTTCGGAAATAGCACTTGCTATGGAACAAGAAGTTAGTGTCAAAGAATTAGCTTACACAATTCACCCACACCCAACTTATTCGGAAATTATTTGA", "aa": "MHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNPEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEII", "aa_hexdigest": "d8f4e719c5e8344aab4ebb095ab15cdc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A223MB31", "query_cov": 0.8602739726027397, "subject_cov": 0.9661538461538461, "identity": 0.513, "score": 315.0, "evalue": 4.05e-103, "valid": false, "product": "Dihydrolipoyl dehydrogenase", "uniref50_id": "UniRef50_A0A223MB31" }, "pscc": { "uniref50_id": "UniRef50_A0A223MB31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [], "id": "EFPKPENHBP_1597", "locus": "GCA000186005_01586" }, { "type": "cds", "sequence": "contig_1", "start": 878174, "stop": 878275, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATCAACTGATGTTTTAGAAACCTTAACCGATGCTGCTGGTTATGGTATTGTTGGGAACTTAGATAAATTAAAGATAGATCTACAAAAAACATGA", "aa": "MLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKT", "aa_hexdigest": "f965803749b04d3a897180f271242a87", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3565.094500000001, "isoelectric_point": 4.777965354919433 }, "id": "EFPKPENHBP_1598", "locus": "GCA000186005_01587" }, { "type": "cds", "sequence": "contig_1", "start": 878318, "stop": 879226, "strand": "-", "frame": 3, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "EC:1.8.1.4", "GO:0004148", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_A0A449B5U3", "UniRef:UniRef90_C4XFF7" ], "nt": "ATGTACGTATTTAAATTTGCTGATATAGGTGAAGGATTACACGAAGGAACAGTTGGTGAAATCAGTGTTAAAGTTGGTGACAAGATTAAAGAAGGAGATACACTTTTTTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAGAATGAAGACTGGTGATGTAATTCATGTAGGACAAGAAATATTTGTAATTGATGATGGCAAAGGTGGCTCAGTAGCTGCAGCTCCTGCTACTAAAGTTGAAGAATCGAAAAGTACACCTGCTACTTCATCATCTACAAGCTTTTATGTTTTTAAATTTGCTGACATTGGTGAAGGTTTACACGAAGGAACAGTTGGTGAAATTCCTATTAAAATAGGTGATAAAGTAAAAGAAGGCGACACATTATTCTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAAAATGAAAACAGGTGATGTAATTCATGTAGGACAAGAAGTTATTGTTATTGATGCAGGAAGTAAAAATCCTACTTCTTCTTCAGTTTCATCTGCACCAAGTTCCAATAAAGAAGAAGAAAAATGTTTAGTAGGTGATGCTCCAAATTCAAGTGAATTAATAAATCTAGATTTCAGTTCACTAAATTCACCCGCAAAAGAAACTCCTCAACCAGTTGCACCTCAAGCTTCAACTTATGAAGCTAACCAAGAAGAAGAAAGCAATGGTGTTTCATTTACTTCTGAAGGTAAAAAATACGAAGGCAAAGTTGATGAAGAATTTGATGTAATTATTGTAGGTAGTGGTCCTGGAGGATATTTAGCCGCTGAAGAATTAGGCAAAGCAGGCAAAAAAGTATTAATTGTTGAAAAAGAATTCTGA", "aa": "MYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSTPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGDAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEF", "aa_hexdigest": "7bd56a70058c6a0d9ba9ccd993c394c8", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "lpdA" ], "truncated": "5-prime", "pseudogene": { "start": 878015, "stop": 879232, "observations": { "mutation": [ 878176, 878320 ], "stop": [ 878176, 878320 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF7", "query_cov": 1.0, "subject_cov": 0.4092140921409214, "identity": 0.997, "score": 508.0, "evalue": 3.26e-174, "gene_start": 1, "gene_end": 302, "reference_start": 3, "reference_end": 304, "reference_sequence": "MNMYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSRPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGDAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEFWGGVCLNIWCIPTKAMLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKTWVKMHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNTEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEIIWEAARAASLKLYLEENKK" }, "paralog": false, "identity": 0.9876543209876543, "score": 718.0, "evalue": 7.62e-259, "description": "Internal stop codon at 878176, 878320. Nonsense mutation around 878176, 878320.", "psc": { "uniref90_id": "UniRef90_C4XFF7", "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_A0A449B5U3", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF7", "EC:1.8.1.4", "UniRef:UniRef50_A0A449B5U3", "KEGG:K00382", "GO:0004148", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5U3" ], "product": "Dihydrolipoyl dehydrogenase" } }, "id": "EFPKPENHBP_1599", "locus": "GCA000186005_01588" }, { "type": "cds", "sequence": "contig_1", "start": 879309, "stop": 879821, "strand": "-", "frame": 2, "gene": null, "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197", "UniRef:UniRef90_C4XFF6" ], "nt": "ATGACTGCTCTTTGGGACTTACGTTCAAGAATCAAAGATATTGTACTTAAAAGTGATAATGTAAAAATTACATTCTTACCTTATATTATTAAAGCAGCTGCTATTGCTTTAAGAGATTTTCCAGTATTCACTGCTAAATATAATGAAGCAAATCAAACTTTAGATTACCCTGGTGTAATTAATGTTGGTATTGCTGTTGATACTGAAGCCGGTTTAATGGTTCCTGTTATTAATAATGCTGATAATTTAAGTATTGTTAATATTGCTAGTGAAGTAACTAGACTTGCAGGCGCTGCAAGAAATAGAACAATTAAACCTGCTGAAATGAAAGGTGCCGGTTTTACTATTACTAACTATGGTTCAGTTGGATCACTTTTTGGAGTTCCTGTTATTAACTATCCAGAATTAGCTATTGGTGGTGTTGGTGCTATTATTGACAAGCCTGTAGTTAAAAATGGACAAGTTGTACCTGGAAAAGTTATGTACCTAACTGTCGCTGCTGATCACCGTTGA", "aa": "MTALWDLRSRIKDIVLKSDNVKITFLPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIASEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHR", "aa_hexdigest": "b8d0a3d66b2f42876d1e5f0b879e22bb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 879228, "stop": 880121, "observations": { "mutation": [ 879311, 879863 ], "stop": [ 879311, 879863 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF6", "query_cov": 1.0, "subject_cov": 0.5362776025236593, "identity": 1.0, "score": 327.0, "evalue": 3.77e-111, "gene_start": 1, "gene_end": 170, "reference_start": 121, "reference_end": 290, "reference_sequence": "MMKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISGAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNSWSNVAYTNLVHEIDMTALWDLRSRIKDIVLKSDNVKITFLPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIASEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHRWIDGAVIGRFASRIKELLEKPDVLGVY" }, "paralog": false, "identity": 0.9932659932659933, "score": 571.0, "evalue": 1.09e-209, "description": "Internal stop codon at 879311, 879863. Nonsense mutation around 879311, 879863.", "psc": { "uniref90_id": "UniRef90_C4XFF6", "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF6", "UniRef:UniRef50_A0A449B197" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197" ], "product": "Pyruvate dehydrogenase E2 component (Dihydrolipoamide acetyltransferase)" } }, "id": "EFPKPENHBP_1600", "locus": "GCA000186005_01589" }, { "type": "cds", "sequence": "contig_1", "start": 879861, "stop": 880178, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAATCAACCCCTATTGCAAGAGCTATGGCTGCTAAACTTGGAATTGATATTAACTTAGTTCCAGGTTCAGGAATTGATGGCCGTATTCTTATAGAAGATATTCAAAAATTTAAAAACAGTCCTATTTCAGGTGCTGCTCATTCAAACTTTGCACCAGCTCCAGTAGCTGATAATAAAGTAATTACTCAACCTAGTGCAGTAGCTCCTAAAGCTACTCCAGCACCTCGTCCTGCCATTGATCGTTCAGCTCACTCAGAAGCTATTACTCCAATTAGAAAAGCAATTTCTAAAGCTATGACTAACTCATGA", "aa": "MKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISGAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNS", "aa_hexdigest": "4cf7105c98f0f1806126bdfc34695a65", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10904.566699999983, "isoelectric_point": 10.28098278045654 }, "id": "EFPKPENHBP_1601", "locus": "GCA000186005_01590" }, { "type": "cds", "sequence": "contig_1", "start": 880301, "stop": 880891, "strand": "-", "frame": 3, "gene": null, "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "nt": "ATGATTTTAAGAATGCCTATGGGTGGTGGAATTAAAGCTATGGAACATCACTCAGAAGCTTTAGAAGCTATTTATGCTCACATTCCAGGTGTTAAAGTTGTTATGCCAGCCTTTCCTTATGATGTTAAAGGTTTATTATTAGCAGCTTTAAATGATCCAGATCCAGTAGTATTCTTAGAAAATAAGAAAATTTATCGTGCTGGGAAACAAGAAGTTCCAGCTGGCGAATATACAGTTGAAATTGGAAAAGCTAATGTCTTAACTCAAGGTAATGACTTAACTTTAGTAACTTATGGAGCTCAAGTATTTGACTCAATTAATGCTGTTAAAAAATATAAAGAAATCAATCCTAACGCTTCAATTGAATTAATTGATTTAAGAACAATTAAACCTCTTGATACTAAAACTATTGTTGAAAGTGTTAAGAAAACAGGACGTTTATTAGTAGTTCATGAAGCTGTTAAATCATTTTCAGTTTCTTCTGAAATTATGGCTAGAGTTAATGAAAAAGCATTTGAATATCTTAAAGCACCTATGACAAGATGTACAGGTTATGATGTAACTGTTCCTCTTGCAAAAGGCGAAGCATGA", "aa": "MILRMPMGGGIKAMEHHSEALEAIYAHIPGVKVVMPAFPYDVKGLLLAALNDPDPVVFLENKKIYRAGKQEVPAGEYTVEIGKANVLTQGNDLTLVTYGAQVFDSINAVKKYKEINPNASIELIDLRTIKPLDTKTIVESVKKTGRLLVVHEAVKSFSVSSEIMARVNEKAFEYLKAPMTRCTGYDVTVPLAKGEA", "aa_hexdigest": "d40c9db939a38851a1139fa7c3de90c4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A2H6AHZ3", "query_cov": 0.8520408163265306, "subject_cov": 0.8743455497382199, "identity": 0.503, "score": 167.0, "evalue": 3.5e-49, "valid": false, "product": "Pyruvate dehydrogenase E1 component subunit beta", "uniref50_id": "UniRef50_A0A2H6AHZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6AHZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" }, "genes": [], "id": "EFPKPENHBP_1602", "locus": "GCA000186005_01591" }, { "type": "cds", "sequence": "contig_1", "start": 880925, "stop": 881239, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "ATGGCTGAAGAAAAATTAACTTTAAATAATGTTCAAGCAGTTAATAATGCTTTAGACATTGCTATGGCTAAAGACCCCCGTGTAGTTTGTTATGGTGAAGATGCTGGTGTTGAAGGTGGAGTTTTCAGAGCTACAGAAGGCTTACAAAAAAAATATGGTAAAAGTAGAGTATTTGATACCCCTATTTCAGAAGCAACAATTGCTGGTACCGCAATTGGTGCTGCAGTAGCAGGTCTTAGACCTATTGCCGAAATTCAATTCCAAGGTTTTAGTTATCCAGCTATGCAACAATTATTTACTCATGCAGCTAGATGA", "aa": "MAEEKLTLNNVQAVNNALDIAMAKDPRVVCYGEDAGVEGGVFRATEGLQKKYGKSRVFDTPISEATIAGTAIGAAVAGLRPIAEIQFQGFSYPAMQQLFTHAAR", "aa_hexdigest": "0e2861927209bd36f7e5d66cd7ade891", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 94, "aa_cov": 0.8942307692307693, "hmm_cov": 0.9787234042553191, "evalue": 1.113183011324632e-21, "score": 73.55757904052734, "start": 10, "stop": 102 } ], "seq_stats": { "molecular_weight": 11074.48989999999, "isoelectric_point": 5.746047019958497 }, "id": "EFPKPENHBP_1603", "locus": "GCA000186005_01592" }, { "type": "cds", "sequence": "contig_1", "start": 881779, "stop": 881946, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCCGGTTAATATTGTTATTGCAACTCAAATTTCACAATGTGCTGGTATTGCTTATGCACAAAAACAACTTAAAACTGGTGGTGTAGCTATTTCATTTATTGGAAATGGCGGAACTACTGAAGGAGAATTTAGCGAAGGTGTTAATATTGCAACTGTTCAAGAATGA", "aa": "MPVNIVIATQISQCAGIAYAQKQLKTGGVAISFIGNGGTTEGEFSEGVNIATVQE", "aa_hexdigest": "d2b0ffa0a9c9fc647b7662acfb9fff92", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 53, "aa_cov": 0.9636363636363636, "hmm_cov": 1.0, "evalue": 3.3756397138154423e-12, "score": 42.23418045043945, "start": 2, "stop": 54 } ], "seq_stats": { "molecular_weight": 5613.2896, "isoelectric_point": 4.686511421203612 }, "id": "EFPKPENHBP_1604", "locus": "GCA000186005_01593" }, { "type": "cds", "sequence": "contig_1", "start": 882067, "stop": 882201, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGGTACTATCTAGACAACAAGATACATACATGTTGCAATTGCAACGTCAAGGACGTATGCTTACATTTCCACCTAACTTAGGTGAAGAAGCATTACAAATTGCAACAGCAATGGCTATGGAAAAGAAAGACTGA", "aa": "MVLSRQQDTYMLQLQRQGRMLTFPPNLGEEALQIATAMAMEKKD", "aa_hexdigest": "b6971b78d97cddeeb60895f45add9df0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 44, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.0296219833725353e-06, "score": 24.226940155029297, "start": 1, "stop": 44 } ], "seq_stats": { "molecular_weight": 5083.906400000002, "isoelectric_point": 6.0970277786254865 }, "id": "EFPKPENHBP_1605", "locus": "GCA000186005_01594" }, { "type": "cds", "sequence": "contig_1", "start": 882202, "stop": 882351, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAGTACAAATATGTCGATGTTAATAAAATTATGGACGATCCAAATAAGATTATTAGACATTTAGACATAGATGGTAAAGTAATTGAAAAAAATTACAAAGCACCACTAAGTAATGCTGAAGTTTTAGAAGCTTACAAATGA", "aa": "MKTKYKYVDVNKIMDDPNKIIRHLDIDGKVIEKNYKAPLSNAEVLEAYK", "aa_hexdigest": "ccdf004ab974bcd80bdb658e793756f4", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5753.6466, "isoelectric_point": 8.92650203704834 }, "id": "EFPKPENHBP_1606", "locus": "GCA000186005_01595" }, { "type": "cds", "sequence": "contig_1", "start": 882613, "stop": 883251, "strand": "+", "frame": 1, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3", "UniRef:UniRef90_C4XFF3" ], "nt": "ATGAAAAATATTGATTATAAAAGAATTTTTAATAAAGGTATCCCCACAAATATTATTAACTTGTTGACTGAAATATATGAATATAAAGGAAGACAAAACATTTATATCAATAACAAGAATTTAGAAATCCAAAAGCTTAAAAAACAAGCTTTAATTGACAGTGCAGTTTATTCAAATAAAATAGAAAACATCAATATAAGCAAAAAAAGAATTAAAGATATTTTGCTTGAAAATGATGAGTTGCAAAATGAAAATGAAGAAGATATTAAAGGTTATTTTGAATTATTAAATTCTCTATATAATCAAGAAACATTTGAATTATTAAACACTAATTTGATTCTTGATCTTCATTATCAATTGTTTCAAAACTCAAATACCAAAATGAGTGGGCGTTTTAAGTTAAATGATAATTTTATTTTAGAAACAACCTTAGAAGGTACTCAAGAAATAAAATTTATTCCTGTCAAAGCATTTTTTATTAGTGAATATATAAATAATTTATGTAATAAATATAATGAAGAATATAAAAGTAAGAAGATTAATTCATTGCTTCTAACAACAGCTTTTGTTTTGGATTTTATTTGCATTTCGCCTTTTAATCACGGCAATAGCAGAATGTCTAGAATTCTTTTAAATTGA", "aa": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLN", "aa_hexdigest": "6769c60eb1042ae27a758429ca2b00fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 882613, "stop": 883554, "observations": { "mutation": [ 883249, 883360 ], "stop": [ 883249, 883360 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF3", "query_cov": 1.0, "subject_cov": 0.6074498567335244, "identity": 1.0, "score": 414.0, "evalue": 5.27e-144, "gene_start": 1, "gene_end": 212, "reference_start": 1, "reference_end": 212, "reference_sequence": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLNWLLSVGDIKVVQYISLEKIIFENLEKYQNVQQKSSYKWHDNLNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH" }, "paralog": false, "identity": 0.9936102236421726, "score": 610.0, "evalue": 1.08e-223, "description": "Internal stop codon at 883249, 883360. Nonsense mutation around 883249, 883360.", "psc": { "uniref90_id": "UniRef90_C4XFF3", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF3", "UniRef:UniRef50_C4XFF3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3" ], "product": "Fido domain-containing protein" } }, "id": "EFPKPENHBP_1607", "locus": "GCA000186005_01596" }, { "type": "cds", "sequence": "contig_1", "start": 883372, "stop": 883662, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAACTATTATTACTTTATAGAGTTTATTTTAAAAATTGTTTTACAAGCTTATAAAGAATTTGAAAGCCGTTTTACAAGTTTAGAAAATAAAAAAATTGATGTAGCGGACAACATTTTAAAACTGATAGCTCAATATCAAAAAGTAAGTAAAAATGAGCTAATTAATTTACTACCTGAAAGCAGTAAAAGAACAATTGAAAGAAAACTAAATGTCTTATTAAAAGCTAAAAAAATACTTAAAATTAATCAAGGCAAAAATACTAAATACACTATAAAACAGCATTAA", "aa": "MNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH", "aa_hexdigest": "d10838ab1d375a70a886bae245ca4225", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11452.370099999986, "isoelectric_point": 9.838729667663571 }, "id": "EFPKPENHBP_1608", "locus": "GCA000186005_01597" }, { "type": "cds", "sequence": "contig_1", "start": 883753, "stop": 884148, "strand": "+", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "RefSeq:WP_013527087.1", "SO:0001217", "UniParc:UPI0001F59893", "UniRef:UniRef100_A0AB32XCZ8", "UniRef:UniRef50_C4XFF2", "UniRef:UniRef90_C4XFF2" ], "nt": "ATGAAAAGAAAAACAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTTAACAAGAGATGTAAGTGATTTTTATGTTTAG", "aa": "MKRKTINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKNYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMLTRDVSDFYV", "aa_hexdigest": "6aba0f396b5a0295c53662afccb873c5", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59893", "ncbi_nrp_id": "WP_013527087.1", "uniref100_id": "UniRef100_A0AB32XCZ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59893", "RefSeq:WP_013527087.1", "UniRef:UniRef100_A0AB32XCZ8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCZ8", "uniref90_id": "UniRef90_C4XFF2" }, "psc": { "uniref90_id": "UniRef90_C4XFF2", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XFF2" }, "pscc": { "uniref50_id": "UniRef50_C4XFF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF2" ], "product": "Mutator family transposase" }, "genes": [], "id": "EFPKPENHBP_1609", "locus": "GCA000186005_01598" }, { "type": "cds", "sequence": "contig_1", "start": 884405, "stop": 884671, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "b1d2697471a467a119612aa745a6f757", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 9.227899805118591e-09, "score": 32.01397705078125, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10129.612099999993, "isoelectric_point": 8.806848716735843 }, "id": "EFPKPENHBP_1610", "locus": "GCA000186005_01599" }, { "type": "cds", "sequence": "contig_1", "start": 884732, "stop": 884935, "strand": "+", "frame": 2, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3", "UniRef:UniRef90_C4XFB3" ], "nt": "ATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAA", "aa": "MFDYSLEIRTLIYTTNSIENLNSVFRKYTKTKTVFPSDDSLLKILFLASQQIIKNEVIQELEIDLAF", "aa_hexdigest": "2117f64c331567bfe1a192fe98a2620d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 884579, "stop": 884973, "observations": { "deletion": [ 884891 ], "mutation": [ 884669, 884702, 884714 ], "stop": [ 884669, 884702, 884714, 884897, 884921 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB3", "query_cov": 0.8059701492537313, "subject_cov": 0.4122137404580153, "identity": 0.9440000000000001, "score": 99.0, "evalue": 3.02e-25, "gene_start": 1, "gene_end": 54, "reference_start": 52, "reference_end": 105, "reference_sequence": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDKWGKKYPTSISIWRQNWSEISTMFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKKWSNSRIRNWSSILNEFKIIDFENEE" }, "paralog": false, "identity": 0.9236641221374046, "score": 224.0, "evalue": 3.57e-77, "description": "Internal stop codon at 884669, 884702, 884714, 884897, 884921. Frameshift due to deletion around 884891. Nonsense mutation around 884669, 884702, 884714.", "psc": { "uniref90_id": "UniRef90_C4XFB3", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB3", "UniRef:UniRef50_C4XFB3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3" ], "product": "Mutator family transposase" } }, "id": "EFPKPENHBP_1611", "locus": "GCA000186005_01600" }, { "type": "cds", "sequence": "contig_1", "start": 885193, "stop": 885537, "strand": "+", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD77", "UniRef:UniRef90_A0AB32XD77" ], "nt": "TTGAAAAAAGGAGAGACTTTACACAAAATTCTAAACAGTCTCAATTTATCAAAAAACAATGATAATAATCTCGTTTTAGAACATTTAAAAACAACTGATTTTTCTTCAAATTTTATTATTCATTCAGATCATGGCTATCAATATTCATCAAATGATTATGTAAAATTCATATCATCAAAAAATGAAAAAATCTCAATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGA", "aa": "MKKGETLHKILNSLNLSKNNDNNLVLEHLKTTDFSSNFIIHSDHGYQYSSNDYVKFISSKNEKISMSRVGNSLYNREIEYFFSILKTEIFPNFCKRVKLLTFNELKNEIKEFID", "aa_hexdigest": "bae413de12e69e14fcdb5b700d6c2807", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 885193, "stop": 885630, "observations": { "mutation": [ 885535 ], "stop": [ 885535 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD77", "query_cov": 1.0, "subject_cov": 0.7862068965517242, "identity": 1.0, "score": 225.0, "evalue": 2.59e-74, "gene_start": 1, "gene_end": 114, "reference_start": 1, "reference_end": 114, "reference_sequence": "MKKGETLHKILNSLNLSKNNDNNLVLEHLKTTDFSSNFIIHSDHGYQYSSNDYVKFISSKNEKISMSRVGNSLYNREIEYFFSILKTEIFPNFCKRVKLLTFNELKNEIKEFIDWYNNDRFMKKLDLKTPQQMWDVYKNNKFIGV" }, "paralog": false, "identity": 0.9862068965517241, "score": 293.0, "evalue": 1.99e-103, "description": "Internal stop codon at 885535. Nonsense mutation around 885535.", "psc": { "uniref90_id": "UniRef90_A0AB32XD77", "product": "Transposase", "uniref50_id": "UniRef50_A0AB32XD77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD77", "UniRef:UniRef50_A0AB32XD77" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD77" ], "product": "Transposase" } }, "id": "EFPKPENHBP_1612", "locus": "GCA000186005_01601" }, { "type": "cds", "sequence": "contig_1", "start": 885796, "stop": 885975, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCY6", "UniRef:UniRef90_A0AB32XCY6" ], "nt": "ATGATTGATACATATCAAACTATCTTTGAATTAAAAAATATTATAAAATATATTAAAAAAGATTTAAAGTTAAATAAAGTTTCTCTAAAAGCTTTCAAAGGCAAAATAAATGGAACTAATATAAAAAGGTGTGTTATAAGATTCTCTACGTTTTCTTATTTCGTAATTATTTTATCATAA", "aa": "MIDTYQTIFELKNIIKYIKKDLKLNKVSLKAFKGKINGTNIKRCVIRFSTFSYFVIILS", "aa_hexdigest": "a949703136f717b0cbfa0ed1baccf9af", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0AB32XCY6", "query_cov": 1.0, "subject_cov": 0.855072463768116, "identity": 1.0, "score": 113.0, "evalue": 6.05e-32, "valid": true, "uniref50_id": "UniRef50_A0AB32XCY6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCY6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6948.327899999998, "isoelectric_point": 10.074361610412598 }, "id": "EFPKPENHBP_1613", "locus": "GCA000186005_01602" }, { "type": "cds", "sequence": "contig_1", "start": 886535, "stop": 887281, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0", "UniRef:UniRef90_C4XFB0" ], "nt": "ATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGA", "aa": "MYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLE", "aa_hexdigest": "d325320591f32ac6814f3d3679059197", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 886283, "stop": 887455, "observations": { "mutation": [ 886450, 886537, 887401 ], "stop": [ 886450, 886537, 887401 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB0", "query_cov": 1.0, "subject_cov": 0.6358974358974359, "identity": 1.0, "score": 491.0, "evalue": 3.51e-173, "gene_start": 1, "gene_end": 248, "reference_start": 59, "reference_end": 306, "reference_sequence": "MIMEGAVKNKMSKTLADNWNAQAEVFKNSEERKIKYNVERKIERDIFIWGETVRIIYYMYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLEWITKQEKNKTVYANAKKFKKYYKENREYIWNSVLVKNYFGGCAETVVGHDLKRFCSKKFATFSLKTIQLSIAKNMETKANLFFI" }, "paralog": false, "identity": 0.9897435897435898, "score": 768.0, "evalue": 5.17e-285, "description": "Internal stop codon at 886450, 886537, 887401. Nonsense mutation around 886450, 886537, 887401.", "psc": { "uniref90_id": "UniRef90_C4XFB0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB0", "UniRef:UniRef50_C4XFB0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0" ], "product": "ISLre2 family transposase" } }, "id": "EFPKPENHBP_1614", "locus": "GCA000186005_01603" }, { "type": "cds", "sequence": "contig_1", "start": 887698, "stop": 888147, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_C4XFA9" ], "nt": "TTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGA", "aa": "MLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHND", "aa_hexdigest": "d11fea40fd11324cc7c0bad85465fa78", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 887497, "stop": 888438, "observations": { "mutation": [ 887700, 888204, 888408 ], "stop": [ 887700, 888204, 888408 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA9", "query_cov": 1.0, "subject_cov": 0.476038338658147, "identity": 0.993, "score": 280.0, "evalue": 6.88e-93, "gene_start": 1, "gene_end": 149, "reference_start": 98, "reference_end": 246, "reference_sequence": "MYNFYMSLELWRPLKITNPLLEDIFHLNIDTKFKIHPLIIFENTKNKTYYCIRLQTARKSSSKYNLIIDNSSYQTNEYWKNHESVVVTKDIFIIDKELLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHNDWKDLDKTKDNVNIYSFENIGFLSKSSKSESSYIDPYDNKNIEKAKDKVAKEIPIKESKTDKDKTKLK" }, "paralog": false, "identity": 0.987220447284345, "score": 610.0, "evalue": 1.67e-225, "description": "Internal stop codon at 887700, 888204, 888408. Nonsense mutation around 887700, 888204, 888408.", "psc": { "uniref90_id": "UniRef90_C4XFA9", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA9", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17230.18999999998, "isoelectric_point": 8.557549476623539 }, "id": "EFPKPENHBP_1615", "locus": "GCA000186005_01604" }, { "type": "cds", "sequence": "contig_1", "start": 889013, "stop": 889162, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTTCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAA", "aa": "MNKKGFKAGKSKKQVSIKKFKEIQIYKKDRIKAKPVVLSDEEINKLFAK", "aa_hexdigest": "df2560211445a3f631e2ef9afa6784c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5709.8361, "isoelectric_point": 10.22006015777588 }, "id": "EFPKPENHBP_1616", "locus": "GCA000186005_01605" }, { "type": "cds", "sequence": "contig_1", "start": 889580, "stop": 890272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGA", "aa": "MRGTGKYINHSITFNLKNSAYHVYYPPIDLSSLDIQKHFEIEMGKNISNDSKELKEKIRNKLIGLIEEQFNLYKNKTGIEMLLNKDIKVEGLDNVLDLLVQGNKFKDGIDIKIKGINANLISSTKIHIINDTEAPAFDLSLLNDTLVKNPIVISQKEPEKITKDIINKLNEITIASGIEFKKDYLVSLVNASFYLLGLVADIDKLNEAKYEKFKKVYEETKAKVMAEYNT", "aa_hexdigest": "765f71697b058a00437b3fa17058fe4a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26199.983000000033, "isoelectric_point": 6.923068809509277 }, "id": "EFPKPENHBP_1617", "locus": "GCA000186005_01606" }, { "type": "cds", "sequence": "contig_1", "start": 890375, "stop": 890542, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGA", "aa": "MNYGDETNEAIKENYRRQVKEIVLNFVKRNIANSAKLKAIGITENEINGGWVIAN", "aa_hexdigest": "81fb4d5b440c05c797eb70b5ddfcd8b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.0062, "isoelectric_point": 8.06539783477783 }, "id": "EFPKPENHBP_1618", "locus": "GCA000186005_01607" }, { "type": "cds", "sequence": "contig_1", "start": 890567, "stop": 890665, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGA", "aa": "MISLYKNFVDQASKFNVETGNEYENIFKDKIT", "aa_hexdigest": "1f15fa2f5e0a93065b5e0a705db0eb3e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3787.208700000001, "isoelectric_point": 4.93575038909912 }, "id": "EFPKPENHBP_1619", "locus": "GCA000186005_01608" }, { "type": "cds", "sequence": "contig_1", "start": 890747, "stop": 891211, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGA", "aa": "MFSDELKEQLRFVYPNIKITTDKADLGIYAEANVLGKGALRNLVVDKVLESSSRYYKCLLFRKENDKYVEIGRKEQKLIEIPVNNGTSEYSYMSEEGIWLLFTVNEKSISNVNLVLIDAKSEPKSFFLDSIENKIEKINNQSEDYFEAFLDGGN", "aa_hexdigest": "14f3e53a5efaf9d6cffd2a93f9497e12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17724.8618, "isoelectric_point": 4.860722923278809 }, "id": "EFPKPENHBP_1620", "locus": "GCA000186005_01609" }, { "type": "cds", "sequence": "contig_1", "start": 891224, "stop": 891367, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGA", "aa": "MDQKQIISPYMLDSKGKELKDKEGKLIVNKNYDPSIDKETGTKKQIV", "aa_hexdigest": "819168f07f3459a3efeb395e0df65970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5396.195300000002, "isoelectric_point": 8.96376476287842 }, "id": "EFPKPENHBP_1621", "locus": "GCA000186005_01610" }, { "type": "cds", "sequence": "contig_1", "start": 891383, "stop": 891658, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGA", "aa": "MVNKQKVSVLNRIFEKKLIDERVNVNDASKIPFDKNSGSGNGEGDVAKNEYLVEVYRYKKGSNNSGEPEEKYVVIYEVIGNQFKQNIKYYA", "aa_hexdigest": "57d3445016ece3a9d5ba8fc5ce158842", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10439.608, "isoelectric_point": 8.752115058898926 }, "id": "EFPKPENHBP_1622", "locus": "GCA000186005_01611" }, { "type": "cds", "sequence": "contig_1", "start": 891734, "stop": 891934, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGA", "aa": "MYKRLSGASLASRLTISTSITNPDGSISQIEAKTKANGGDLKNLIKRDEPERVSNKELEKDFVGKP", "aa_hexdigest": "e3d98ceff45638283a1d2f30053658e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.081999999999, "isoelectric_point": 9.453788948059085 }, "id": "EFPKPENHBP_1623", "locus": "GCA000186005_01612" }, { "type": "cds", "sequence": "contig_1", "start": 892061, "stop": 892411, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTCACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGA", "aa": "MNRFEYGVNKKIDQNHYSIDLLSDTLGDFTTSVDGVFKDNGGKSNIEHLNKIFDKEYEFEYASGIKVKYKYDYKIKNEAVGEKNKIDVYIKVLSVKIDDKEIIDKDLDKILNNIED", "aa_hexdigest": "3b51870aa20a7ab6eaf58d735c44552d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13509.014199999981, "isoelectric_point": 5.077279472351074 }, "id": "EFPKPENHBP_1624", "locus": "GCA000186005_01613" }, { "type": "cds", "sequence": "contig_1", "start": 892454, "stop": 892705, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGA", "aa": "MEEHKGASFTNLFAEYKVSQKTLEFLNNLETDKDKRVYSGLTDDKNKMFLWGLHDFDCEIEGMYDVRYYNNKVLNNLEYAKVG", "aa_hexdigest": "9bda0e8721a59f6b7453f6ce3f3a7db8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9826.907499999998, "isoelectric_point": 5.06073932647705 }, "id": "EFPKPENHBP_1625", "locus": "GCA000186005_01614" }, { "type": "cds", "sequence": "contig_1", "start": 892817, "stop": 893278, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGA", "aa": "MHAEARAKNEEKMNKKKMLKLIPLSVLSIAIPSVLNLSLNTNKKLLGYGKDFKAPEEKHKIDTNSLFSNFTSMEDDEIAKNERLQNRYQVKWSQLCDKGLLYAQDQWVGYHKTGPVKNKITSIVSRPTLGSFEVRYEKAYYTNPSNHNSYGWF", "aa_hexdigest": "547e92dcc2bb3430ecf9e1292e14eeb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17690.019999999993, "isoelectric_point": 9.477900123596193 }, "id": "EFPKPENHBP_1626", "locus": "GCA000186005_01615" }, { "type": "cds", "sequence": "contig_1", "start": 893324, "stop": 893719, "strand": "-", "frame": 3, "gene": null, "product": "ICEF Integrative Conjugal Element-II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7", "UniRef:UniRef90_C4XFA7" ], "nt": "TTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAA", "aa": "MTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK", "aa_hexdigest": "1e201de3510ce021ade96f9646eff4b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 893324, "stop": 894007, "observations": { "mutation": [ 893728, 893998 ], "stop": [ 893728, 893998 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA7", "query_cov": 1.0, "subject_cov": 0.5745614035087719, "identity": 0.992, "score": 242.0, "evalue": 2.31e-79, "gene_start": 1, "gene_end": 131, "reference_start": 98, "reference_end": 228, "reference_sequence": "MKTWEISQENKKGDKLYVKSSRASKNGSKCISTRLSEPDYVALYERWVKRIKQSGSTPYVELKDIIRNALIKQEKEEIAKDFKNDLFYALRKVSWASLTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK" }, "paralog": false, "identity": 0.9780701754385965, "score": 429.0, "evalue": 1.42e-155, "description": "Internal stop codon at 893728, 893998. Nonsense mutation around 893728, 893998.", "psc": { "uniref90_id": "UniRef90_C4XFA7", "product": "ICEF Integrative Conjugal Element-II", "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA7", "UniRef:UniRef50_C4XFA7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7" ], "product": "ICEF Integrative Conjugal Element-II" } }, "id": "EFPKPENHBP_1627", "locus": "GCA000186005_01616" }, { "type": "cds", "sequence": "contig_1", "start": 893988, "stop": 894794, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ], "nt": "ATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MDDLIKQMKKFVAPKDHHDKEGYTKKVLDMAELLEGKFGRYGTLGALYNGHTNINIENDLVVFKTSNLIDTEGSVNARIGIMVLISLVNGFIFDNAFNNKKRINEYKEKNKIRLISKKEIQKLTRYCSFCIDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "fd4e47e07cfb58f2a4c9ea07298295fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F2982C0", "query_cov": 0.9776119402985075, "subject_cov": 0.9225352112676056, "identity": 0.622, "score": 325.0, "evalue": 4.81e-109, "valid": false, "uniref50_id": "UniRef50_UPI001F2982C0" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2982C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30615.79740000003, "isoelectric_point": 8.78963565826416 }, "id": "EFPKPENHBP_1628", "locus": "GCA000186005_01617" }, { "type": "cds", "sequence": "contig_1", "start": 894801, "stop": 895247, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGA", "aa": "MFSLTSSRANHNVMILGTSGMGKSTLTKKILTSSLAAGNIAIIIDPQAEYVKWANKMGGQIVDLGSGDGTVINPLQVKSFIAREEDDANVNFNTLINNHLLWLEKFFSFIFDDMNEMKKNILQLEIIKLYQSWGIYRLKEVKELKNDG", "aa_hexdigest": "7bdeadcb03d81e2d1d7547110e406fd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 49, "aa_cov": 0.3310810810810811, "hmm_cov": 1.0, "evalue": 6.643206648327057e-08, "score": 28.243488311767578, "start": 9, "stop": 57 }, { "id": "PF01935.23", "name": "DUF87", "length": 108, "aa_cov": 0.722972972972973, "hmm_cov": 0.7777777777777778, "evalue": 2.353833018396146e-10, "score": 37.21625900268555, "start": 12, "stop": 118 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 76, "aa_cov": 0.5135135135135135, "hmm_cov": 0.881578947368421, "evalue": 2.441558326642471e-08, "score": 29.528656005859375, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 16628.021899999992, "isoelectric_point": 6.732430839538575 }, "id": "EFPKPENHBP_1629", "locus": "GCA000186005_01618" }, { "type": "cds", "sequence": "contig_1", "start": 895251, "stop": 895664, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGA", "aa": "MDAGNLDNSIINRFGSALEEESIAVMIDQIQADNFKLFDSNIFVLAEAENAEELKQNNLKIKNGFTRSNFGINDLTFRQFEGFLDMSNYPLSKLDREYYQITSINNAIGYPLKNLPLNDGNNLLLGNEVGGNDSPIV", "aa_hexdigest": "60571d7151425553be189582d3abe5bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15326.890099999975, "isoelectric_point": 4.264652442932129 }, "id": "EFPKPENHBP_1630", "locus": "GCA000186005_01619" }, { "type": "cds", "sequence": "contig_1", "start": 895770, "stop": 896117, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGA", "aa": "MIETFYLILNAPTLEELDECYSTFIQKAEDYEVISYEKLSGFELLSFLNKLNHFNKSEEDIETFLNESDIYESEYSLDRLFKYEKVEFKSNSLKIDDKLYSVKCAGNIPIRLNNE", "aa_hexdigest": "7329907af2238aaf3f1e5143e18b2cfa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13622.117199999982, "isoelectric_point": 4.459382820129394 }, "id": "EFPKPENHBP_1631", "locus": "GCA000186005_01620" }, { "type": "cds", "sequence": "contig_1", "start": 896172, "stop": 896336, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGATGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGA", "aa": "MVLIENFARILDSFKYKFSIVKLDEQIDYRRNLNYINTIQSKKMLNSGGETKEF", "aa_hexdigest": "7eabf1fafeeaa9103dfce5aa5be32540", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.395699999999, "isoelectric_point": 9.098954963684083 }, "id": "EFPKPENHBP_1632", "locus": "GCA000186005_01621" }, { "type": "cds", "sequence": "contig_1", "start": 896358, "stop": 896561, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGA", "aa": "MFKFKAMPKKYSNVVDDKNVRDSSDLNPYSTLVENDVVKNKSFSNKLFQKSEHSNYFSVFKLGGKNI", "aa_hexdigest": "da513e353c81c917ec7655a69dc05219", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7754.720499999998, "isoelectric_point": 9.601872825622557 }, "id": "EFPKPENHBP_1633", "locus": "GCA000186005_01622" }, { "type": "cds", "sequence": "contig_1", "start": 897088, "stop": 897528, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAA", "aa": "MNICVKNRFTAPKKAKSICSPRKNTPTDNIFPTYLSFHILTFLPSSSKLKGKIIGNIKYKKNNGNIFPMASIPPKTSGIAINTAPIIDRNAAVIKMDAQLPMFKCSVFFAKATKLPKLISKLIVKPKGFVVAGIKISLTSPANFGG", "aa_hexdigest": "be119545e9f8a00836c1c4d3d042829e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15852.893799999973, "isoelectric_point": 10.454209327697754 }, "id": "EFPKPENHBP_1634", "locus": "GCA000186005_01623" }, { "type": "cds", "sequence": "contig_1", "start": 897381, "stop": 897692, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGA", "aa": "MRSALVFSVKSLFILLAFQITILLFNLFVQKIITLMIGEGNDIVVAFINGLHRLFYPPKLAGDVKDILIPATTNPFGFTINLDISFGSFVALAKKTEHLNIGN", "aa_hexdigest": "1f530bd3ff05364bbc363b2280c88bc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11381.43519999998, "isoelectric_point": 9.223765754699706 }, "id": "EFPKPENHBP_1635", "locus": "GCA000186005_01624" }, { "type": "cds", "sequence": "contig_1", "start": 897759, "stop": 898007, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGA", "aa": "MSGFFTRVMEIFKYGPTYVLYLLLFFVSLIVFILLAILLSVVKLATFDLLNFVIFGIMPRQNILDHQSPYMYVRFAIVSAVI", "aa_hexdigest": "594dbf9addb063641ab0662d22f90068", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9502.483199999995, "isoelectric_point": 9.222605323791505 }, "id": "EFPKPENHBP_1636", "locus": "GCA000186005_01625" }, { "type": "cds", "sequence": "contig_1", "start": 898097, "stop": 898288, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0395 family pilin-like conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4", "UniRef:UniRef90_C4XFA4" ], "nt": "ATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGA", "aa": "MKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKIL", "aa_hexdigest": "5087b9cbe03a20493b3e281279e3d943", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 898007, "stop": 898294, "observations": { "mutation": [ 898063, 898099 ], "stop": [ 898063, 898099 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA4", "query_cov": 1.0, "subject_cov": 0.6631578947368421, "identity": 1.0, "score": 117.0, "evalue": 4.48e-33, "gene_start": 1, "gene_end": 63, "reference_start": 3, "reference_end": 65, "reference_sequence": "MKMKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKILWAGIVLILVIVGWGIFELVISIQASGGKTS" }, "paralog": false, "identity": 0.968421052631579, "score": 172.0, "evalue": 2.82e-57, "description": "Internal stop codon at 898063, 898099. Nonsense mutation around 898063, 898099.", "psc": { "uniref90_id": "UniRef90_C4XFA4", "product": "Mbov_0395 family pilin-like conjugal transfer protein", "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA4", "UniRef:UniRef50_C4XFA4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4" ] } }, "id": "EFPKPENHBP_1637", "locus": "GCA000186005_01626" }, { "type": "cds", "sequence": "contig_1", "start": 898460, "stop": 898666, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "EFPKPENHBP_1638", "locus": "GCA000186005_01627" }, { "type": "cds", "sequence": "contig_1", "start": 898670, "stop": 899035, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEQVYRNYFKEYLDKATNALLDVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDNQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "013ce869a4b1cb22bf3d6b450ba5ea08", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14177.287199999993, "isoelectric_point": 5.539209938049315 }, "id": "EFPKPENHBP_1639", "locus": "GCA000186005_01628" }, { "type": "cds", "sequence": "contig_1", "start": 899060, "stop": 899386, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVRKHYHYYFDMKLDNYYEQFINDFALKGTSKTFKDIAIDGLYKQGMWNNYFDLGKDYSKWFYLAFTRSNVEKYHEGFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "46c6c097a53fd654c69c00bd8f34eab2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13261.713499999989, "isoelectric_point": 5.872456932067871 }, "id": "EFPKPENHBP_1640", "locus": "GCA000186005_01629" }, { "type": "cds", "sequence": "contig_1", "start": 899387, "stop": 899650, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGSQKDVFAYKDSYHGNK", "aa_hexdigest": "ea19c6d5ae4af4eb3676eadbf877fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.355799999998, "isoelectric_point": 4.955473518371581 }, "id": "EFPKPENHBP_1641", "locus": "GCA000186005_01630" }, { "type": "cds", "sequence": "contig_1", "start": 899666, "stop": 899971, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MKKSKKFMKFMAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKYDADVYRNDN", "aa_hexdigest": "5ae4a8cbd911d43c80787cf35874157b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11541.700899999993, "isoelectric_point": 5.83141918182373 }, "id": "EFPKPENHBP_1642", "locus": "GCA000186005_01631" }, { "type": "cds", "sequence": "contig_1", "start": 900052, "stop": 900249, "strand": "-", "frame": 1, "gene": null, "product": "ICEF-IA ORF13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945", "UniRef:UniRef90_Q8G945" ], "nt": "ATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGA", "aa": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVY", "aa_hexdigest": "37a7ed9202ae80cc53604b2722443644", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 899995, "stop": 900249, "observations": { "mutation": [ 900030, 900054 ], "stop": [ 900030, 900054 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8G945", "query_cov": 1.0, "subject_cov": 0.7738095238095238, "identity": 1.0, "score": 122.0, "evalue": 3.68e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVYWVKKRIEYWKQSKKNSDLH" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.29e-53, "description": "Internal stop codon at 900030, 900054. Nonsense mutation around 900030, 900054.", "psc": { "uniref90_id": "UniRef90_Q8G945", "product": "ICEF-IA ORF13", "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8G945", "UniRef:UniRef50_Q8G945" ] }, "pscc": { "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945" ], "product": "ICEF-IA ORF13" } }, "id": "EFPKPENHBP_1643", "locus": "GCA000186005_01632" }, { "type": "cds", "sequence": "contig_1", "start": 900249, "stop": 900800, "strand": "-", "frame": 2, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "RefSeq:WP_013526867.1", "SO:0001217", "UniParc:UPI00000C02C3", "UniRef:UniRef100_C4XFA2", "UniRef:UniRef50_C4XFA2", "UniRef:UniRef90_C4XFA2" ], "nt": "ATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAA", "aa": "MNKVILTGRVVSEDYFKAKTTSGKAVLGFVIAVNNEYGGEPDFLNITAFGLQASNFEKYNKKGDLVEVIGKIKRNAYVDRDNHKQYKMEIVADNIIYSAKKVDRNAEGNDEDIIYKDETKLPKEEKEKVVKARLKKKDEMMEPFKQYIGMDPLCQSVYDKYEEEAFNKDVEVKDETKKHGQTL", "aa_hexdigest": "7876c7aa53c01bbcd9e8e8b7b6e1a631", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02C3", "ncbi_nrp_id": "WP_013526867.1", "uniref100_id": "UniRef100_C4XFA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C3", "RefSeq:WP_013526867.1", "UniRef:UniRef100_C4XFA2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA2", "uniref90_id": "UniRef90_C4XFA2" }, "psc": { "uniref90_id": "UniRef90_C4XFA2", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_C4XFA2" }, "pscc": { "uniref50_id": "UniRef50_C4XFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA2" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "EFPKPENHBP_1644", "locus": "GCA000186005_01633" }, { "type": "cds", "sequence": "contig_1", "start": 900810, "stop": 901526, "strand": "-", "frame": 2, "gene": null, "product": "Mbov_0392 family ICE element protein", "db_xrefs": [ "RefSeq:WP_013526868.1", "SO:0001217", "UniParc:UPI00000C0686", "UniRef:UniRef100_C4XFA1", "UniRef:UniRef50_C4XFA1", "UniRef:UniRef90_C4XFA1" ], "nt": "ATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAA", "aa": "MIEKVFCFKDLYYLTQYALSDISDEEVVAKFNYLAKNIKNNTMRDKYKKQVLSIVDKMSNVDDDINQKIFDKLFYVEDTNEKNLEQYITLLNICNVEDLENIENNSLDLKFSFNDFFGENGYIESEYLFDLVDEDLKQNGINSKYIYSLGDLSRIDTEYVYIDDYLNFHPEDIDDIKKEYIENLASLKLDEIIDLNNKESIDANNKVEITDKTVKEAQKHVAGIVEKESETAKVKNTK", "aa_hexdigest": "7a9c47a621909a378797adedb1d43586", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C0686", "ncbi_nrp_id": "WP_013526868.1", "uniref100_id": "UniRef100_C4XFA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0686", "RefSeq:WP_013526868.1", "UniRef:UniRef100_C4XFA1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA1", "uniref90_id": "UniRef90_C4XFA1" }, "psc": { "uniref90_id": "UniRef90_C4XFA1", "product": "Mbov_0392 family ICE element protein", "uniref50_id": "UniRef50_C4XFA1" }, "pscc": { "uniref50_id": "UniRef50_C4XFA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA1" ] }, "genes": [], "id": "EFPKPENHBP_1645", "locus": "GCA000186005_01634" }, { "type": "cds", "sequence": "contig_1", "start": 901539, "stop": 901778, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526869.1", "SO:0001217", "UniParc:UPI00000C02C2", "UniRef:UniRef100_C4XFA0", "UniRef:UniRef50_C4XFA0", "UniRef:UniRef90_C4XFA0" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEEFKKMEPEKYEKFRKESNKKICNKLFAK", "aa_hexdigest": "9df3ce0167d8cced414b46ed0b0c03a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02C2", "ncbi_nrp_id": "WP_013526869.1", "uniref100_id": "UniRef100_C4XFA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C2", "RefSeq:WP_013526869.1", "UniRef:UniRef100_C4XFA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA0", "uniref90_id": "UniRef90_C4XFA0" }, "psc": { "uniref90_id": "UniRef90_C4XFA0", "uniref50_id": "UniRef50_C4XFA0" }, "pscc": { "uniref50_id": "UniRef50_C4XFA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9481.049399999993, "isoelectric_point": 9.631012535095213 }, "id": "EFPKPENHBP_1646", "locus": "GCA000186005_01635" }, { "type": "cds", "sequence": "contig_1", "start": 901787, "stop": 902734, "strand": "-", "frame": 3, "gene": null, "product": "MORN repeat protein", "db_xrefs": [ "RefSeq:WP_013526824.1", "SO:0001217", "UniParc:UPI00000C02A4", "UniRef:UniRef100_C4XF99", "UniRef:UniRef50_C4XF99", "UniRef:UniRef90_C4XF99" ], "nt": "ATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCTAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MQVFNALENEEIINRRNYEKNGTNFELISTKSGLTLAHNVLNDNSQEYYEMNEGAFSGRYYKIAENNQLVGVANYQNDELNGEATIYNTKGDILYKAHFEDGLLNGAVEEYKDGALVKSTMYENGFKTGEEVVYDAKGEVEGKTNFITNNEVKEKEVKEYKEADKEKKAEIRNKNFKEVLKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "0cdba05f663e8efc85d8182828c25163", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A4", "ncbi_nrp_id": "WP_013526824.1", "uniref100_id": "UniRef100_C4XF99", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A4", "RefSeq:WP_013526824.1", "UniRef:UniRef100_C4XF99" ] }, "ips": { "uniref100_id": "UniRef100_C4XF99", "uniref90_id": "UniRef90_C4XF99" }, "psc": { "uniref90_id": "UniRef90_C4XF99", "product": "MORN repeat protein", "uniref50_id": "UniRef50_C4XF99" }, "pscc": { "uniref50_id": "UniRef50_C4XF99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF99" ], "product": "MORN repeat protein" }, "genes": [], "id": "EFPKPENHBP_1647", "locus": "GCA000186005_01636" }, { "type": "cds", "sequence": "contig_1", "start": 902736, "stop": 902924, "strand": "-", "frame": 2, "gene": null, "product": "ICEF-IA ORF6", "db_xrefs": [ "RefSeq:WP_013526825.1", "SO:0001217", "UniParc:UPI00000C073C", "UniRef:UniRef100_Q8G8G5", "UniRef:UniRef50_Q8G8G5", "UniRef:UniRef90_Q8G8G5" ], "nt": "ATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAA", "aa": "MKRYFYRIRVKKNLESKKTKSYAVCSNFNEADKIANEIIEKGDYGWVQVERVEKIKHYFKEV", "aa_hexdigest": "536d7d5d695db8bc2e435e625cfadd0d", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00000C073C", "ncbi_nrp_id": "WP_013526825.1", "uniref100_id": "UniRef100_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C073C", "RefSeq:WP_013526825.1", "UniRef:UniRef100_Q8G8G5" ] }, "ips": { "uniref100_id": "UniRef100_Q8G8G5", "uniref90_id": "UniRef90_Q8G8G5" }, "psc": { "uniref90_id": "UniRef90_Q8G8G5", "product": "ICEF-IA ORF6", "uniref50_id": "UniRef50_Q8G8G5" }, "pscc": { "uniref50_id": "UniRef50_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G8G5" ], "product": "ICEF-IA ORF6" }, "genes": [], "id": "EFPKPENHBP_1648", "locus": "GCA000186005_01637" }, { "type": "cds", "sequence": "contig_1", "start": 903067, "stop": 903294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGA", "aa": "MPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALA", "aa_hexdigest": "a7bce82dcd65675848f6e9422130e73d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8724.933399999994, "isoelectric_point": 9.221702766418456 }, "id": "EFPKPENHBP_1649", "locus": "GCA000186005_01638" }, { "type": "cds", "sequence": "contig_1", "start": 903307, "stop": 903891, "strand": "-", "frame": 1, "gene": "traD", "product": "TraM recognition site of TraD and TraG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1", "UniRef:UniRef90_A0AB32XBH1" ], "nt": "ATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAGCTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGA", "aa": "MLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSANGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNES", "aa_hexdigest": "f918f8a0bf6604f81688e687aaff7c58", "start_type": "ATG", "rbs_motif": null, "genes": [ "traD" ], "truncated": "both", "pseudogene": { "start": 903004, "stop": 904173, "observations": { "mutation": [ 903069, 903309, 904128 ], "stop": [ 903069, 903309, 904128 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBH1", "query_cov": 1.0, "subject_cov": 0.470873786407767, "identity": 1.0, "score": 343.0, "evalue": 1.9e-115, "gene_start": 1, "gene_end": 194, "reference_start": 95, "reference_end": 288, "reference_sequence": "MSICYINAETNKRYYWAELFKNVKTIANIVPETLTGNLSHTTSALKNFTEDEFIKRLTRETKSFDLHGIVSSNEPYVIFIHYPDHKPSNHFLVSMLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNESWNYQDLPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALAWQKILSFQQKYKNKLSSELVNDFTKSINICLDQMNDIKTAGAKT" }, "paralog": false, "identity": 0.9897172236503856, "score": 728.0, "evalue": 1.37e-269, "description": "Internal stop codon at 903069, 903309, 904128. Nonsense mutation around 903069, 903309, 904128.", "psc": { "uniref90_id": "UniRef90_A0AB32XBH1", "product": "TraM recognition site of TraD and TraG", "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBH1", "UniRef:UniRef50_A0AB32XBH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1" ] } }, "id": "EFPKPENHBP_1650", "locus": "GCA000186005_01639" }, { "type": "cds", "sequence": "contig_1", "start": 904192, "stop": 904332, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTGA", "aa": "MWVDNAKLIVNVVAQFLLLLSIDRPDLLPERHFNLQSVASMCNLDT", "aa_hexdigest": "1994a274baad84226fc2088274757087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5254.1116, "isoelectric_point": 4.74869327545166 }, "id": "EFPKPENHBP_1651", "locus": "GCA000186005_01640" }, { "type": "cds", "sequence": "contig_1", "start": 904417, "stop": 904719, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGA", "aa": "MNEPESDINSVVLGGVGSGKTQRVLLPNIIYNSHLRYKYRANFCIPDPKKEIIKMVGKKLEKQGYKIYAVDFSDTKKSVGWNPLSFAYKLAHRVPEGHPK", "aa_hexdigest": "6390e01b408d03e3933cb69dff4b3dd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11349.04659999999, "isoelectric_point": 9.68065319061279 }, "id": "EFPKPENHBP_1652", "locus": "GCA000186005_01641" }, { "type": "cds", "sequence": "contig_1", "start": 904858, "stop": 905154, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTTCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGA", "aa": "MGDKKKKILKISLSAVAILLAAIIIFLALAIIFNVKRLKSFKMVSIKDFFAQTKASIYAFPKQFLISYAFLLIASIAGISYLFYKNKLKTSLLKPNKY", "aa_hexdigest": "a7ee8fb1bcb6146dfc81f181ec136612", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11069.498099999988, "isoelectric_point": 10.299291801452636 }, "id": "EFPKPENHBP_1653", "locus": "GCA000186005_01642" }, { "type": "cds", "sequence": "contig_1", "start": 905154, "stop": 905375, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAA", "aa": "MVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ", "aa_hexdigest": "4e375f5225f369e001680770108e444f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 905154, "stop": 905624, "observations": { "mutation": [ 905411 ], "stop": [ 905411 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.46794871794871795, "identity": 1.0, "score": 147.0, "evalue": 3.96e-44, "gene_start": 1, "gene_end": 73, "reference_start": 84, "reference_end": 156, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 8.41e-107, "description": "Internal stop codon at 905411. Nonsense mutation around 905411.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8364.290099999997, "isoelectric_point": 8.664953804016115 }, "id": "EFPKPENHBP_1654", "locus": "GCA000186005_01643" }, { "type": "cds", "sequence": "contig_1", "start": 905409, "stop": 905621, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGGAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAACAATATGCCATTAAGAAACGTAGCACTTGTATTGTTTTCAATATCAATTTTAGTTTTCTTCATATTTGCTAGCCTAGTGAATGTATTAAATATAAGTTTTGCTTGA", "aa": "MGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFA", "aa_hexdigest": "0635f0269fe1de7e3229df2928cd1cab", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 905154, "stop": 905624, "observations": { "mutation": [ 905411 ], "stop": [ 905411 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.44871794871794873, "identity": 1.0, "score": 131.0, "evalue": 1.21e-37, "gene_start": 1, "gene_end": 70, "reference_start": 2, "reference_end": 71, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 7.52e-107, "description": "Internal stop codon at 905411. Nonsense mutation around 905411.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7962.459199999995, "isoelectric_point": 9.601679420471193 }, "id": "EFPKPENHBP_1655", "locus": "GCA000186005_01644" }, { "type": "cds", "sequence": "contig_1", "start": 905715, "stop": 906056, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENMFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ca03355bf950c1fafc7488b9f7c761da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13355.14579999998, "isoelectric_point": 9.442893791198731 }, "id": "EFPKPENHBP_1656", "locus": "GCA000186005_01645" }, { "type": "cds", "sequence": "contig_1", "start": 906162, "stop": 906302, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "EFPKPENHBP_1657", "locus": "GCA000186005_01646" }, { "type": "cds", "sequence": "contig_1", "start": 906420, "stop": 906593, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGNLGLEHNVLTAND", "aa_hexdigest": "2e7232a416963aea6061be918ee46d1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.762199999999, "isoelectric_point": 4.0500284194946286 }, "id": "EFPKPENHBP_1658", "locus": "GCA000186005_01647" }, { "type": "cds", "sequence": "contig_1", "start": 906854, "stop": 907045, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526872.1", "SO:0001217", "UniParc:UPI00000C0690", "UniRef:UniRef100_C4XF95", "UniRef:UniRef50_C4XF95", "UniRef:UniRef90_C4XF95" ], "nt": "ATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAG", "aa": "MKYKEGQLILVNYKFAQKFLSSSVGDDFLYLGLQNRKNLAKRLSQIMNIPKLTPEFHCIAKGS", "aa_hexdigest": "4e10fe405d634def018d3530d31e48fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C0690", "ncbi_nrp_id": "WP_013526872.1", "uniref100_id": "UniRef100_C4XF95", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0690", "RefSeq:WP_013526872.1", "UniRef:UniRef100_C4XF95" ] }, "ips": { "uniref100_id": "UniRef100_C4XF95", "uniref90_id": "UniRef90_C4XF95" }, "psc": { "uniref90_id": "UniRef90_C4XF95", "uniref50_id": "UniRef50_C4XF95" }, "pscc": { "uniref50_id": "UniRef50_C4XF95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF95" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.454599999999, "isoelectric_point": 9.779418754577634 }, "id": "EFPKPENHBP_1659", "locus": "GCA000186005_01648" }, { "type": "cds", "sequence": "contig_1", "start": 907525, "stop": 907995, "strand": "-", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACACTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKHYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANL", "aa_hexdigest": "0ce2f59f08c4a7324d20ec487f422a68", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 907276, "stop": 908058, "observations": { "mutation": [ 907506, 907527 ], "stop": [ 907506, 907527 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 0.9940000000000001, "score": 301.0, "evalue": 5.37e-102, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9884615384615385, "score": 499.0, "evalue": 3.68e-182, "description": "Internal stop codon at 907506, 907527. Nonsense mutation around 907506, 907527.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "EFPKPENHBP_1660", "locus": "GCA000186005_01649" }, { "type": "cds", "sequence": "contig_1", "start": 908589, "stop": 908861, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12" ], "nt": "ATGATCGAACCTAAGGTTTTACTTTTAGACGAACCTGAAGCAAATTTGGATCCTTATGCAAGAAATCAACTTTATCAAAGACTTCAAGCTTTAAAGCAAAAGGGCGTGTCAATAATTTTAGCATCTCACGTTATAGATTCAAATAATGAAATAATTGATTACATTACTATTTTGAACGAAAAGGAAATAGTATTTGAATCAAAAATAAACTCAGTAAAAGAATCATCTAGCATTTATGAAAATTTTAATAAAGAAATATATGATAAGATTTAA", "aa": "MIEPKVLLLDEPEANLDPYARNQLYQRLQALKQKGVSIILASHVIDSNNEIIDYITILNEKEIVFESKINSVKESSSIYENFNKEIYDKI", "aa_hexdigest": "2fc80ea22c636a358b4386f44b814cba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 40, "aa_cov": 0.4444444444444444, "hmm_cov": 1.0, "evalue": 1.3999523887585012e-09, "score": 34.73923873901367, "start": 4, "stop": 43 } ], "seq_stats": { "molecular_weight": 10441.781599999993, "isoelectric_point": 4.774384498596191 }, "id": "EFPKPENHBP_1661", "locus": "GCA000186005_01650" }, { "type": "cds", "sequence": "contig_1", "start": 908965, "stop": 909243, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTAAAATAATAAGAAATTTAAGTATTTTATCACCAATACCAATGGTTTTGAATTCATGTTCAAATACCGATAAGCCTAAAAAACAAGAGGAACAAGAGGAAAAAAAAGTAAAAGAATATCATGTTCCGACTTCTTGCGTAGATTATGACAAAGATGAATTAAAAAATATATTAGAAAAAAATACTAAATTAGTAAAAAATGTTAATACAAATAAAAAAGAAAAATATATTGATTATGATTTCGTTTATGACGTTATACAAAAATGA", "aa": "MKKIIKIIRNLSILSPIPMVLNSCSNTDKPKKQEEQEEKKVKEYHVPTSCVDYDKDELKNILEKNTKLVKNVNTNKKEKYIDYDFVYDVIQK", "aa_hexdigest": "23f0fd9532dbe95eeb41542216cbed66", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10904.566399999992, "isoelectric_point": 8.985297203063965 }, "id": "EFPKPENHBP_1662", "locus": "GCA000186005_01651" }, { "type": "cds", "sequence": "contig_1", "start": 909354, "stop": 909662, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATCAAATACAAATTCGCAATTATTTAACAAAGAAATTCTAGAAAATTCTCTTTTTCAATCAAAATTATCATTTGAATTCATAGTTAAAAAGTTTGACGGCATTACTATTTTTTTGAATTTTTTTATATCTTTTTTGTTTTTTCTTAATTCGATAGCTTTTTCTATTTCTGATTTAATTTTTTTTAAATTTTCGTCTTCACTTAATTTAAAATTTCTATTTGAATATTTTATTTTAGCTTCTTCTTCATTGTTGTTTATTCAATTTAATTCACTTATCAAATCATCTTTAGATGAAAATAAATAA", "aa": "MESNTNSQLFNKEILENSLFQSKLSFEFIVKKFDGITIFLNFFISFLFFLNSIAFSISDLIFFKFSSSLNLKFLFEYFILASSSLLFIQFNSLIKSSLDENK", "aa_hexdigest": "f2c7cd8557c5ee9728189e2de00d6607", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11935.688599999981, "isoelectric_point": 5.25041103363037 }, "id": "EFPKPENHBP_1663", "locus": "GCA000186005_01652" }, { "type": "cds", "sequence": "contig_1", "start": 909781, "stop": 910449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTGGAATAAAAACATCTATTTTTATTTAAATAATTTTATAGACGAAGAAAAAGATATTATTGTTGATAAAAATATATTTAATATGGAAAGTTATGAACAATTTAATAATGTAAAAATAAGAAATCTATATTTAAGAAATTTTAAATATAGTTTTAATACAGGTGGTTTAAATTTAGATTATTTAAATATTGCAAAAAAATTAAAAGTAAAAAAACTTATAATTGATAAGGATTTATCATTTAAAACATCTTTCAAATTATCCTTAAAAAACACAGATTTCGAGGACATAGAAGGTGCAGAATATTTAATTAAAAACTTATATGTTCCGGAATTAAGTGGAGGAAACTCTGATTTATTTATAAAATTATTGAAAAATAATAAAATTCCTAAATCTTTCAAGATTAAATTAAAAGATTATTCGTTTCCACCAGAAGAAAATGATTTATACGTTAATTGTCTTTATCGTTTATTTAATTTAAATTTAGATTTAGTAGATTTATCCGATTCTGATCCTGTAAATATTTTATATTTTGGAAACCCTAAAAATAGTCAAACCGATAATGATGCATTTAACTGTTATACAGATAAAGTAATTTATCCTAAAAAAGTTGAAAAGGTTGAGGGGGCAATAGCTGCGCATAGATTTAAAAATATTAAATTTTGA", "aa": "MSWNKNIYFYLNNFIDEEKDIIVDKNIFNMESYEQFNNVKIRNLYLRNFKYSFNTGGLNLDYLNIAKKLKVKKLIIDKDLSFKTSFKLSLKNTDFEDIEGAEYLIKNLYVPELSGGNSDLFIKLLKNNKIPKSFKIKLKDYSFPPEENDLYVNCLYRLFNLNLDLVDLSDSDPVNILYFGNPKNSQTDNDAFNCYTDKVIYPKKVEKVEGAIAAHRFKNIKF", "aa_hexdigest": "0d24f8feb620baa6388e230457778c91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26123.635300000027, "isoelectric_point": 8.557742881774903 }, "id": "EFPKPENHBP_1664", "locus": "GCA000186005_01653" }, { "type": "cds", "sequence": "contig_1", "start": 910627, "stop": 910869, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTATAAAAATAAATAATTTAACAGCTTCAACGTTAAATCCAATTTATAATATCACACTCGGCAAGGAATATTTTGATGCAAGTCATTACAAAGATATTCTGGACGTTAACACATTAAATTTTACATTTGCTGAAAAAAGTTTAATGGGCTTTAAAACTGTTAGATGCTTAAATGATAATTTTGATGTATTTAAATCGTTAAATATGACAAAAGTAAATCACGAAGGAAAAGAAAAGTGA", "aa": "MTIKINNLTASTLNPIYNITLGKEYFDASHYKDILDVNTLNFTFAEKSLMGFKTVRCLNDNFDVFKSLNMTKVNHEGKEK", "aa_hexdigest": "73d55457139a8fe0920ec00f1f4aa43b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9210.458599999998, "isoelectric_point": 7.853490257263184 }, "id": "EFPKPENHBP_1665", "locus": "GCA000186005_01654" }, { "type": "cds", "sequence": "contig_1", "start": 910924, "stop": 911067, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAACGAATTAAGCTTTACATTTGAATGTAAAGATGGAACTTATTTGTTGAAACCAGATGAAGATCCTTATTTTGCAAAAAATGGTGAATTTTCATCAAGAAATTATTTAACTTACAATATTCAAAAAATTAAATAA", "aa": "MKYNELSFTFECKDGTYLLKPDEDPYFAKNGEFSSRNYLTYNIQKIK", "aa_hexdigest": "80eb07601e6a0004a3c6edc11ee5f710", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5651.2928, "isoelectric_point": 6.104871559143065 }, "id": "EFPKPENHBP_1666", "locus": "GCA000186005_01655" }, { "type": "cds", "sequence": "contig_1", "start": 911149, "stop": 911298, "strand": "+", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCR3", "UniRef:UniRef90_A0AB32XCR3" ], "nt": "ATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAAATTTGA", "aa": "MKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEI", "aa_hexdigest": "f6d7f30bccc956d5602e5994b7946921", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0AB32XCR3", "query_cov": 1.0, "subject_cov": 0.98, "identity": 1.0, "score": 99.4, "evalue": 1.08e-26, "valid": true, "product": "Transposase", "uniref50_id": "UniRef50_A0AB32XCR3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCR3" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_1667", "locus": "GCA000186005_01656" }, { "type": "cds", "sequence": "contig_1", "start": 911313, "stop": 911624, "strand": "+", "frame": 3, "gene": null, "product": "Transposase, mutator type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS1", "UniRef:UniRef90_A0AB32XCS1" ], "nt": "ATGAAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGATGCAAGATTATGA", "aa": "MKKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARL", "aa_hexdigest": "e84299d7f15d54d900b3229c6c5b2f44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 911313, "stop": 911876, "observations": { "mutation": [ 911622, 911802 ], "stop": [ 911622, 911802 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCS1", "query_cov": 1.0, "subject_cov": 0.5508021390374331, "identity": 1.0, "score": 200.0, "evalue": 7.39e-64, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MKKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARLWQERKLETYYPIIYIDAVHFHIVDNNVVTKKATYVIMGINGDGQKEILGLYIGENESAKFWMSVLNALKNRGISKIDIICSQII" }, "paralog": false, "identity": 0.9893048128342246, "score": 365.0, "evalue": 2.97e-131, "description": "Internal stop codon at 911622, 911802. Nonsense mutation around 911622, 911802.", "psc": { "uniref90_id": "UniRef90_A0AB32XCS1", "product": "Transposase, mutator type", "uniref50_id": "UniRef50_A0AB32XCS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCS1", "UniRef:UniRef50_A0AB32XCS1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS1" ], "product": "Transposase, mutator type" } }, "id": "EFPKPENHBP_1668", "locus": "GCA000186005_01657" }, { "type": "cds", "sequence": "contig_1", "start": 911858, "stop": 912073, "strand": "+", "frame": 2, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET7", "UniRef:UniRef90_C4XET7" ], "nt": "TTGCTCACAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MLTDNLKGIQQAIEAVFPNSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "6adb078ae440bcf8fb6cce689e7cc88c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 911858, "stop": 912375, "observations": { "deletion": [ 912293 ], "mutation": [ 912071, 912104, 912116 ], "stop": [ 912071, 912104, 912116, 912299, 912323 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET7", "query_cov": 1.0, "subject_cov": 0.4127906976744186, "identity": 1.0, "score": 144.0, "evalue": 1.38e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MLTDNLKGIQQAIEAVFPNSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDKWGKKYPTSISIWRRNWSEISTMFDYSLEIRTLIYTTNSIENLNSVFRKYTKTKTVFPSDDSLLKILFLASQQIIKKWSNSRIRNWSSILNEFKIIDFENEE" }, "paralog": false, "identity": 0.9593023255813954, "score": 312.0, "evalue": 2.61e-111, "description": "Internal stop codon at 912071, 912104, 912116, 912299, 912323. Frameshift due to deletion around 912293. Nonsense mutation around 912071, 912104, 912116.", "psc": { "uniref90_id": "UniRef90_C4XET7", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XET7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET7", "UniRef:UniRef50_C4XET7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XET7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET7" ], "product": "Mutator family transposase" } }, "id": "EFPKPENHBP_1669", "locus": "GCA000186005_01658" }, { "type": "cds", "sequence": "contig_1", "start": 912134, "stop": 912337, "strand": "+", "frame": 2, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3", "UniRef:UniRef90_C4XFB3" ], "nt": "ATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAA", "aa": "MFDYSLEIRTLIYTTNSIENLNSVFRKYTKTKTVFPSDDSLLKILFLASQQIIKNEVIQELEIDLAF", "aa_hexdigest": "2117f64c331567bfe1a192fe98a2620d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 911981, "stop": 912375, "observations": { "deletion": [ 912293 ], "mutation": [ 912071, 912104, 912116 ], "stop": [ 912071, 912104, 912116, 912299, 912323 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB3", "query_cov": 0.8059701492537313, "subject_cov": 0.4122137404580153, "identity": 0.9440000000000001, "score": 99.0, "evalue": 3.02e-25, "gene_start": 1, "gene_end": 54, "reference_start": 52, "reference_end": 105, "reference_sequence": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDKWGKKYPTSISIWRQNWSEISTMFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKKWSNSRIRNWSSILNEFKIIDFENEE" }, "paralog": false, "identity": 0.9236641221374046, "score": 224.0, "evalue": 3.57e-77, "description": "Internal stop codon at 912071, 912104, 912116, 912299, 912323. Frameshift due to deletion around 912293. Nonsense mutation around 912071, 912104, 912116.", "psc": { "uniref90_id": "UniRef90_C4XFB3", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB3", "UniRef:UniRef50_C4XFB3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3" ], "product": "Mutator family transposase" } }, "id": "EFPKPENHBP_1670", "locus": "GCA000186005_01659" }, { "type": "cds", "sequence": "contig_1", "start": 912664, "stop": 913050, "strand": "-", "frame": 1, "gene": null, "product": "DUF402 domain-containing protein", "db_xrefs": [ "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_D3VRN0", "UniRef:UniRef90_C4XF90" ], "nt": "ATGCCTAAAAATAGTATGTATAATGCCTTGATTTTATTAAAACCAAAAAGTAATTATGTCTATGTAAATTTAGCTTCAAAACCATATTATGAAGATAATACAATCAAATTTGTTGATTTTGATTTAGATGTTAAAAGCTATCCTAAAAAAGATTTTACAATTGTAGATAAAGAAGAATTTCGTTACAATTCTAGACGCCTCAAATATCCTAAAAAACTGATAAATATGATTTACGATAATTTAGAGGAAATAGTTAATAAACATAATAATGGTGAGTATTTCTTTAATCCAGATGTAATTGATTATTACATCAATCAAGCTAAAAAAGACAAATCAATTTATCGTGATTTTAGAGTTAAAAAAGCAAGTAGTTATAGTTTTAAATGA", "aa": "MPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFK", "aa_hexdigest": "44b38b8bc1d95021df16e688c3f81a6c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 912646, "stop": 913341, "observations": { "mutation": [ 912666, 913056, 913080, 913170, 913305 ], "stop": [ 912666, 913056, 913080, 913170, 913305 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF90", "query_cov": 1.0, "subject_cov": 0.5541125541125541, "identity": 1.0, "score": 259.0, "evalue": 3.2e-86, "gene_start": 1, "gene_end": 128, "reference_start": 98, "reference_end": 225, "reference_sequence": "MKNMEYILKGGKWMSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQWNGVKVLRNTPQHYVLFMYKTKVAETDKKGWVYREYVLWFMPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFKWRNKNK" }, "paralog": false, "identity": 0.974025974025974, "score": 451.0, "evalue": 4.21e-164, "description": "Internal stop codon at 912666, 913056, 913080, 913170, 913305. Nonsense mutation around 912666, 913056, 913080, 913170, 913305.", "psc": { "uniref90_id": "UniRef90_C4XF90", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_D3VRN0", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF90", "UniRef:UniRef50_D3VRN0", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRN0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRN0" ], "product": "DUF402 domain-containing protein" } }, "id": "EFPKPENHBP_1671", "locus": "GCA000186005_01660" }, { "type": "cds", "sequence": "contig_1", "start": 913168, "stop": 913302, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAGTTAATAAGGATAATCTTCTTATTAAAGAAGAAAATTTAAAACCTAAAAAAATAGTTTTTCCCTTAATTGGATCAATTATTAATGTCCAAGCTTTTAAATTTAATGGTGAATTATACCGCCAATGA", "aa": "MSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQ", "aa_hexdigest": "a192cefd794e14205ccb8513098c5cb7", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5104.018300000002, "isoelectric_point": 9.601614952087402 }, "id": "EFPKPENHBP_1672", "locus": "GCA000186005_01661" }, { "type": "cds", "sequence": "contig_1", "start": 913289, "stop": 913483, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGATAATGAATTAATCAAAGAATATGAAAAAGATCCATTGCCAGATAGATATTTAACTAGTAGTGATGCACCTAGTGAATTAGATTCAACGAATTCATATAAAATAGAAGCTAGCTTTATAAGCAAAAAACAATCAAATAAAAAATATGGAATATATTTTAAAAGGAGGGAAATGAATGTCTTTAGTTAA", "aa": "MQDNELIKEYEKDPLPDRYLTSSDAPSELDSTNSYKIEASFISKKQSNKKYGIYFKRREMNVFS", "aa_hexdigest": "70ef24e96a18522dcb8d8efb93d804de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7575.3679999999995, "isoelectric_point": 6.205590248107909 }, "id": "EFPKPENHBP_1673", "locus": "GCA000186005_01662" }, { "type": "cds", "sequence": "contig_1", "start": 913508, "stop": 913975, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAAGCTCAATTAGAAGCTGATCGAAACTTTTATAGTAACAATATTTATAAAAGTGAAAATCAACAACTATTTTACAAATTGTTTCTTGAAACAATGGGTTATGGTAAAACTATAGCTCAAGTTAAAAGCAAAATAGATGTTAAAAAATTAATTGCTGGAGACACTGATAGTTATTCAGTTGAAATTGATAAAAATAATATTTTTCAAACAAGAATTAGTGGTTATTTAGATAACAAAAATATCAAAGGATTTTATTTCACAAATAATAAAGATGAAAAAGAGTTTGTTAAAATAAATTATTTAAATGCATTTAAATTTGAGGGAAAAGATAATTTATTAGATACTACTTTTACCTTATCGCCAAATATGGTTTTAAGTGATTTAATTTCAAAACAAGCAGCTTATACAAGTGATTATTTTGATTATAGAAATATAAAAGAAAATTCACCTATTGAATTCTGA", "aa": "MHKAQLEADRNFYSNNIYKSENQQLFYKLFLETMGYGKTIAQVKSKIDVKKLIAGDTDSYSVEIDKNNIFQTRISGYLDNKNIKGFYFTNNKDEKEFVKINYLNAFKFEGKDNLLDTTFTLSPNMVLSDLISKQAAYTSDYFDYRNIKENSPIEF", "aa_hexdigest": "02e5941cf22b06206b414c561ecac536", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18136.189799999986, "isoelectric_point": 6.595448875427247 }, "id": "EFPKPENHBP_1674", "locus": "GCA000186005_01663" }, { "type": "cds", "sequence": "contig_1", "start": 914162, "stop": 914302, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCTATTAAAGATGAAAATGACAAAGTAACTTTTAGAGAAACTAAAGATTTCGATTCAAAATATTTAATAGCCGATTATTCAATAAATAGTTTGCCTTATTATTATTCTCTAGTTGAACTAGTGCCAAGAGAATGA", "aa": "MKSIKDENDKVTFRETKDFDSKYLIADYSINSLPYYYSLVELVPRE", "aa_hexdigest": "574ec02ac4110de8a8b850d17618d522", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.1548, "isoelectric_point": 4.920460700988768 }, "id": "EFPKPENHBP_1675", "locus": "GCA000186005_01664" }, { "type": "cds", "sequence": "contig_1", "start": 914591, "stop": 914797, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGGACCGGAAATAAAATTTTTAGATTCTATAAACGTTAATGACTTTAGTATTATCACTAAAGAATCTGCAGGTATTTATTTGCCAACAATTCAAAGAATTTATTTAAATGGTGCGGCACTAAGTGAAAAAGGAGCAAAATTAAGTGTCAAAGTAAAACAAATACTTTATTCACTTTTTCATGAATACATGCACCATTGA", "aa": "MTYGPEIKFLDSINVNDFSIITKESAGIYLPTIQRIYLNGAALSEKGAKLSVKVKQILYSLFHEYMHH", "aa_hexdigest": "356b010b2c3ef79291ab8d8f2b341330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7746.9088, "isoelectric_point": 8.02297763824463 }, "id": "EFPKPENHBP_1676", "locus": "GCA000186005_01665" }, { "type": "cds", "sequence": "contig_1", "start": 914966, "stop": 915277, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTTTTGTTTCATTGTTTGTTGCTGTTTATTTACTTTTAAATAATAAAAATGAAACAATAAATAAAAATAATAATTTAGTTAAAATAAAAATACAAAGTAAAGAAGATAAAATAGGACTTATAAATCAAGATAAATATATTGAACAATATGCAAAAAGTCCTTCTTCATTAAATAATTTTGACAATGTTACAAATGATTTAAAAAATCAATTTATTGATTTAAAATTTAATGATTATATTTTGGCTAATAACACTGTTTTAAAGTATGATACGAAAAAACATAGCTATAAATATGATAAGAGAAATTGA", "aa": "MCFVSLFVAVYLLLNNKNETINKNNNLVKIKIQSKEDKIGLINQDKYIEQYAKSPSSLNNFDNVTNDLKNQFIDLKFNDYILANNTVLKYDTKKHSYKYDKRN", "aa_hexdigest": "b49c3b105b6b078a6e81cf5fca7a2304", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12145.707299999984, "isoelectric_point": 9.256064414978024 }, "id": "EFPKPENHBP_1677", "locus": "GCA000186005_01666" }, { "type": "cds", "sequence": "contig_1", "start": 915592, "stop": 915807, "strand": "+", "frame": 1, "gene": null, "product": "MAG6790 family protein", "db_xrefs": [ "RefSeq:WP_013354908.1", "SO:0001217", "UniParc:UPI0001E32FAB", "UniRef:UniRef100_C4XF88", "UniRef:UniRef50_C4XF88", "UniRef:UniRef90_C4XF88" ], "nt": "ATGTATAAATTTAAAGCAAAATTAGTTTCAACTCAAGAAGTTGTTGCACAAGCAAACTCACTTGAAGAAATCGAAGGATTAATCCTTGGCTTCCGTCGTAAACAAAAATATGACGAACACACAAGAGCCAACGAAAAAATCCAAATTATACATGTTGAAAGGGATTCTTTAAAAGGTAAACATAAATCAAAAGAAGAAATTCTTAAAGTTGTTTAA", "aa": "MYKFKAKLVSTQEVVAQANSLEEIEGLILGFRRKQKYDEHTRANEKIQIIHVERDSLKGKHKSKEEILKVV", "aa_hexdigest": "3aa7ea5bd06dae26f05da1144afc132b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FAB", "ncbi_nrp_id": "WP_013354908.1", "uniref100_id": "UniRef100_C4XF88", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FAB", "RefSeq:WP_013354908.1", "UniRef:UniRef100_C4XF88" ] }, "ips": { "uniref100_id": "UniRef100_C4XF88", "uniref90_id": "UniRef90_C4XF88" }, "psc": { "uniref90_id": "UniRef90_C4XF88", "product": "MAG6790 family protein", "uniref50_id": "UniRef50_C4XF88" }, "pscc": { "uniref50_id": "UniRef50_C4XF88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF88" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_1678", "locus": "GCA000186005_01667" }, { "type": "cds", "sequence": "contig_1", "start": 915980, "stop": 916219, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAGATTTTTATTAGGTGCTTTAGCTCCAGTTGTTGCTTTAAGCCCTATTGCTATTGCAGTTAGTTGTGGAAAACAACCAACCATTGAAGAACAATTCGCTAAATTAAATAATGAAGGAAAATTAGCCTTTATTAATGCCATTGACTACAAAGGAATTATGTCAATGGATGAAAAAGAAAAATTAATTTCTAAATTAAATAAAGATGCTGCTCAATTTGGATCAATTGTTTGA", "aa": "MKKRFLLGALAPVVALSPIAIAVSCGKQPTIEEQFAKLNNEGKLAFINAIDYKGIMSMDEKEKLISKLNKDAAQFGSIV", "aa_hexdigest": "55e34f3d3ff1294323d022cb6eb7df7a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8567.070599999995, "isoelectric_point": 9.167484855651857 }, "id": "EFPKPENHBP_1679", "locus": "GCA000186005_01668" }, { "type": "cds", "sequence": "contig_1", "start": 916295, "stop": 916669, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCTGTAACCGATGGTTTTGAATATGGTCAAAACTTTAATACAACTAAACAAGTAAATAATGCTGCAACAGATAAATACATTCCAGTTATCTTTATGGACATTGATGAAACTGTTTTACAAAATGACTACACAGAAACATTTGGTATGTTAAATGGTGGTTACAATGGAGACACGAAAGAAAAGAACGACCTTAAAGGAAATAGATTTGCTGTGCCAGGCGCTATTGATTTCATTAACTATGTACAATCAAAAGGTGCTTTAGTTGTTTATAACTCAGATATGAACCAATCTACAGCAGTTAGAGAAGCTGTTAAAAAGAATTTGATAAACTTAGGTGTTAAATATGTTTCAGACTTCCAATTCTGA", "aa": "MKYAVTDGFEYGQNFNTTKQVNNAATDKYIPVIFMDIDETVLQNDYTETFGMLNGGYNGDTKEKNDLKGNRFAVPGAIDFINYVQSKGALVVYNSDMNQSTAVREAVKKNLINLGVKYVSDFQF", "aa_hexdigest": "42ac7cfd0b6ef03e36ecec4ef2d7b217", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13921.399399999984, "isoelectric_point": 4.818377876281737 }, "id": "EFPKPENHBP_1680", "locus": "GCA000186005_01669" }, { "type": "cds", "sequence": "contig_1", "start": 916670, "stop": 916810, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGTTCAATGCCTTATGTAGCAATGGATGGAACTAAAGTTATTACAGACGAAATGACAAAAGATATGTCTAAGGGTGATCTTAAAAGCCTAGCTGAAAGAATGAAATTTACAAAAACTTTTGAAACTACACCATGA", "aa": "MRGSMPYVAMDGTKVITDEMTKDMSKGDLKSLAERMKFTKTFETTP", "aa_hexdigest": "5e824ebab18077695bfcb7de0d322eaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5206.043, "isoelectric_point": 8.064495277404784 }, "id": "EFPKPENHBP_1681", "locus": "GCA000186005_01670" }, { "type": "cds", "sequence": "contig_1", "start": 916961, "stop": 917083, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAGGCGACAACTTTAATGACTTCTTTGATAGAAACTCAAAAGGTAAAGACAATGACAAAAGAACTCAACAATACGTTAAAAACACAGACATGAGAAAATTATTCACAGATTATTAA", "aa": "MRIGDNFNDFFDRNSKGKDNDKRTQQYVKNTDMRKLFTDY", "aa_hexdigest": "b8fbc57b438c5c1af0b50d8b6eb02fc8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4923.374200000001, "isoelectric_point": 9.221509361267088 }, "id": "EFPKPENHBP_1682", "locus": "GCA000186005_01671" }, { "type": "cds", "sequence": "contig_1", "start": 918277, "stop": 918627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGTCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTTTTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALVLPKIFGDLSPFLKYYFNFFIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "097b322b767dca169ee8e2a053647a07", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13641.180399999976, "isoelectric_point": 9.912288093566893 }, "id": "EFPKPENHBP_1683", "locus": "GCA000186005_01672" }, { "type": "tRNA", "sequence": "contig_1", "start": 918826, "stop": 918901, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 78.2, "nt": "GCGTCCTTAGCTCAGGCGGTAGAGCAAATGGCTTTTAACCATTGGGTCGGAGGTTCGAGTCCTCTAGGACGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 918867, 918869 ], "id": "EFPKPENHBP_21", "locus": "GCA000186005_01673" }, { "type": "tRNA", "sequence": "contig_1", "start": 918930, "stop": 919016, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 67.7, "nt": "GCCCAGGTGGCGGAATAGGTAGACGCAAGGGACTTAAAATCCCTCGAACGTTAGTTCGTGCTGGTTCAAGTCCAGTTCTGGGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 918980, 918982 ], "id": "EFPKPENHBP_20", "locus": "GCA000186005_01674" }, { "type": "cds", "sequence": "contig_1", "start": 919177, "stop": 919347, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAATAAAATTTTTAATAGTTTATTATTAACTCCATTAATTATAACTCCTATAACATCTTGTGGTAGTAAGCCAGAAGAAAAAAAGTCATTCCCATTACCCAGATCTTGCACAGATTATGACAAAAAAGAATTGAATAAAATAGTTGAAAAAAATACAAAATGA", "aa": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSFPLPRSCTDYDKKELNKIVEKNTK", "aa_hexdigest": "00075997bb042fceeb69ba3094980dd5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.538000000002, "isoelectric_point": 9.597360038757323 }, "id": "EFPKPENHBP_1684", "locus": "GCA000186005_01675" }, { "type": "cds", "sequence": "contig_1", "start": 919645, "stop": 920469, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF85", "UniRef:UniRef90_C4XF85" ], "nt": "TTGAAAAAAGTCAAAAAAGAAATAGAAAATGAAATTAAAATAAGAGAAAATAAAAGTGATGTTATTTCTTTTAAAAATTTTTGTATTTCTTCAAATTTCTTTTCTTTAGAAACTAATGATTTGCAAAATCCTAAATTTATGAAAAGCATGTTAAGACTTTCTTTGTTGAAAGACTGCAATGTCAACTTTGACTACAGCACTCGAGATACATTCTATTTCTATAAAAGAATTAATATAAAATTATCAGAATTTAATAATGTTTTAATTAGCTCATTCAACAATTCAAAATATTCTAAAAATTACATGTCAGGGATTTATTTCTATTTAAATAATTTTGTAGCAGAGAAACAAAATATTATTGTTGAAAAAAATAATTTTAATTATCGTGAAGCAAATCAATTTCAAAATGTAAAAATAAATAATTTAACATTAAATAATTTTAAACCAGACTTTGAAAACGAATACATTATAGCCAAAAAACTTGAAGTTAATACTATAGAATTTGGTGATGACTTTCTGAATAATGTTCATATAGAAGAAACGTTTAAAAATATAGATTTTAATGACATAAAAGGCGTTCAAAATGTATTAAAAAATTTAGATTGTTCGAAATCGAAAAATAAAGTTTTTCTATTCCAAAAATTAAATAAAATAAATAGAATGCCAGATTCATTTAAAATTAATTTAAGAGAAGAAACATTATCTGATTTTAGTACTTTATGGATGGGCAATTTAGATACCATTTTAGGCGCATGTAAAGATACTCTTGACTTATCAGAATCAAGTTTAATAAAATGTTTATCTTTTGATGATTATGGTATCTGA", "aa": "MKKVKKEIENEIKIRENKSDVISFKNFCISSNFFSLETNDLQNPKFMKSMLRLSLLKDCNVNFDYSTRDTFYFYKRINIKLSEFNNVLISSFNNSKYSKNYMSGIYFYLNNFVAEKQNIIVEKNNFNYREANQFQNVKINNLTLNNFKPDFENEYIIAKKLEVNTIEFGDDFLNNVHIEETFKNIDFNDIKGVQNVLKNLDCSKSKNKVFLFQKLNKINRMPDSFKINLREETLSDFSTLWMGNLDTILGACKDTLDLSESSLIKCLSFDDYGI", "aa_hexdigest": "b2bddd44fc927f4b317fcd6dcfbd543a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 919348, "stop": 920772, "observations": { "mutation": [ 919420, 919570, 920467, 920569, 920623, 920728 ], "stop": [ 919420, 919570, 920467, 920569, 920623, 920728 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF85", "query_cov": 1.0, "subject_cov": 0.4120300751879699, "identity": 0.996, "score": 534.0, "evalue": 1.1e-185, "gene_start": 1, "gene_end": 274, "reference_start": 157, "reference_end": 430, "reference_sequence": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSFPLPRSCTDYDKKELNKIVEKNTKWIENSKTHKLVKYIDYDFFYDVVKKWYCDDNNNLKSDNFYFPVLDKLSVLQKEDDIALVSSYIFSTKKDLQEELDWINNNEKKAIQKYSKRNETLSDEENLKKVKKEIENEIKIRENKSDVISFKNFCISSNFFSLETNDLQNPKFMKSMLRLSLLKDCNVNFDYSTRDTFYFYKRINIKLSEFNNVLISSFNNSKYSKNYMSGIYFYLNNFVAEKQNIIVEKNNFNYREANQFQNVKINNLTLNNFKPDFENEYIIAKKLEVNTIEFGDDFLNNVHIEETFKNIDFNDIKGVQNVLKNLDCSKSKNKVFLFQKLNKINRMPDSFKINLREETLSDFSTLWMGNLDTILGACKDTLDLSESSLIKCLSFDDYGIWGQSFFKTKKIIFPKKIKEIEGQIYPGSFVNIKFWENIGFTSDNFKLFFGRNWRLDEDKQIEIYSFDSSKKFEVLNDAGIFLKDNGEWENKKIRLTIKIDYMESKTLNPIYNITKNMDLFRAENYMDTLDINTFNFELTEKSLMGFKTIRCLKDDFEIFKEKNMEKTTNNNGEEVWYFKNHADYFNVPVNTYPNMEYNKLSFIFECNNGTYTLIPDKVPYFYDGNYLTYNITKIK" }, "paralog": false, "identity": 0.9873417721518988, "score": 931.0, "evalue": 0.0, "description": "Internal stop codon at 919420, 919570, 920467, 920569, 920623, 920728. Nonsense mutation around 919420, 919570, 920467, 920569, 920623, 920728.", "psc": { "uniref90_id": "UniRef90_C4XF85", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XF85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF85", "UniRef:UniRef50_C4XF85" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF85" ], "product": "Lipoprotein" } }, "id": "EFPKPENHBP_1685", "locus": "GCA000186005_01676" }, { "type": "cds", "sequence": "contig_1", "start": 920770, "stop": 920994, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAAAACATTAAATCCTATTTACAATATTACAAAAAATATGGACTTGTTTAGAGCTGAAAATTATATGGACACTTTAGATATTAATACATTTAATTTTGAATTAACAGAGAAAAGTTTGATGGGATTTAAAACAATCAGATGTTTAAAAGATGATTTTGAAATTTTCAAAGAAAAAAATATGGAAAAAACAACAAATAATAATGGAGAAGAAGTTTGA", "aa": "MESKTLNPIYNITKNMDLFRAENYMDTLDINTFNFELTEKSLMGFKTIRCLKDDFEIFKEKNMEKTTNNNGEEV", "aa_hexdigest": "8f8106aa575368676c84cbbdeeb297ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8811.912299999993, "isoelectric_point": 4.732835197448731 }, "id": "EFPKPENHBP_1686", "locus": "GCA000186005_01677" }, { "type": "cds", "sequence": "contig_1", "start": 921049, "stop": 921174, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAATAAATTAAGTTTTATTTTTGAATGCAATAATGGAACATACACATTAATTCCCGACAAAGTACCTTACTTTTACGATGGTAATTATTTAACATATAATATAACAAAAATAAAATAG", "aa": "MEYNKLSFIFECNNGTYTLIPDKVPYFYDGNYLTYNITKIK", "aa_hexdigest": "cfe6e13862270492afbf66182e259e5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.5799000000015, "isoelectric_point": 5.967207527160644 }, "id": "EFPKPENHBP_1687", "locus": "GCA000186005_01678" }, { "type": "cds", "sequence": "contig_1", "start": 921223, "stop": 921447, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAAATATTGATCAAATTAAAGGCAAAACTAAAGAAAAATTATTAGATGAAAAAGATCAAGCTTTTTTATGTAAAGCATTAGCAAAATTAAATTTAAATGTTGATATGAATAAAAACATTTCTGATTACCTATTGAACTTAAACTTAGAAGATGGCTTAGATGCAATGCGTTTTTATGGTTTAAATAAATGCGCAAATTTATTTGAAGAATTGAAATAA", "aa": "MYQNIDQIKGKTKEKLLDEKDQAFLCKALAKLNLNVDMNKNISDYLLNLNLEDGLDAMRFYGLNKCANLFEELK", "aa_hexdigest": "37366898112e2908f1ef2962eab44d26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8586.866399999995, "isoelectric_point": 5.294802284240721 }, "id": "EFPKPENHBP_1688", "locus": "GCA000186005_01679" }, { "type": "cds", "sequence": "contig_1", "start": 921778, "stop": 922107, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02739.22" ], "nt": "ATGAACAAAGACAACTTTTTATTAATAGATGGGAATTATTTATTATTTTCTTCTTTCTATGCTTCATATAATCCGGATAGATTAGAAAATATTATGCGTTCACCACAAGGTTTAACAACTAATGGTGTTCATGTTTTTCTTATGACTTTAAGTAAACTAATAGCATACTTTAAACCTAAATATTTATTTATTGCTTTTGATGCTTATGGAGAAACTAGAAGACATAAAATATTCAAAGATTATAAAGCAGGTAGAACCCAAGCACCAGAAATAATTTTTGAACAATTTAGCTTAATTAAAGAAATTTTGACAAAATTAAATATCAAATGA", "aa": "MNKDNFLLIDGNYLLFSSFYASYNPDRLENIMRSPQGLTTNGVHVFLMTLSKLIAYFKPKYLFIAFDAYGETRRHKIFKDYKAGRTQAPEIIFEQFSLIKEILTKLNIK", "aa_hexdigest": "d88c357d273a1d289f24d6d0aa556f3b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02739.22", "name": "5_3_exonuc_N", "length": 104, "aa_cov": 0.9541284403669725, "hmm_cov": 0.9326923076923077, "evalue": 9.300904913405574e-27, "score": 90.06314849853516, "start": 6, "stop": 109 } ], "seq_stats": { "molecular_weight": 12796.755299999983, "isoelectric_point": 9.486474418640139 }, "id": "EFPKPENHBP_1689", "locus": "GCA000186005_01680" }, { "type": "cds", "sequence": "contig_1", "start": 922122, "stop": 923903, "strand": "-", "frame": 2, "gene": null, "product": "DNA-directed DNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4", "UniRef:UniRef50_UPI00244ECC9B", "UniRef:UniRef90_C4XF83" ], "nt": "ATGGCAATTAGAGATATGGGCAGAGTTATGGGAATTAGCTTAATTGACATCAACCGCATCAGTAAAACTATTAAATCAGATAACTTAACTGATGCTTATAAAAATGATATTGACTTTAAAATTGAAATGGATAATTATCCTGAGTTATATAAACATGCACTTAAAATCGAAGGCTTACCTCGTCAACAAAGTTTCCATGCAGCAGGTTTTATTATTGCTAAGGACAAATTATTTAATTATGTCCCTGTTTGTTTAAGCAATTTAGACAATTACCAACAGGTACAAGTACCAATGAATTACATAGAGAGTTATGGACTTTTAAAAATTGACTTGCTAGGTTTAAGAACTTTAACTGAAATTCAGCTAATTGAAAAAAGTTTAGAAGAAAACCAAAAATTTGATTATTTAGTAAAAAACGATCCTCAAATTTTAAATGATCCTAAAACTTTAACAATGCTCAACTTAGGTTTTACTGAAGGAATATTTCAATTAGAATCTCCGGGCATGAAGAAGACAATTCAAAAAGTTGTCTTGAATTCTTTTGATGATTTATATGCCATTATTTCATTATTTAGACCTGGACCTATGGACTTTATCAATGACTATGCAACTAATAAGAAAAATCAAAAATTAATTAAAAAAATACATCCTGATTATGACAAAGTTGTTGCCAACACATATGGCATAATAGTTTATCAAGAACAAATTATGCAAATAGCTCAAAGTATTGGCGGAATGACTTTTGCTCAAGCTGATTTATTGCGTAAAGCAATTAGCAAAAAAAGTGAAGTTGATATCAAAAAATATAGAAGCATATTTTTTGAAAATGCCATTAAAAAAGGCATAAACGAAAAAATTATTAATGCTATTTATAACAATATTGAAAAATTTGGACTATACGGTTTTAATAAATCTCATGCTGTTAGTTATGCATATTTAACTATGAAAATGTCATACTATAAAGCTCGTTATGCTAACTTATTTTATAGCTCTTTAATTACAAATGCAGCTGGAGCACAAGCTACAATTAATAAATATGTAGCTGAAATTAAGAAACTTAATTTTCCAGTTTATTCGCCAAATATTTTGCATTCAAGTAATAAATGTATAGTAACAAACGATGGCATTTATTTACCTTTTGAAATGATTAAAGGTTTTGGAGCTGAAGGTGTTAATAAAATTGTGGCTGATTTAAATGAAAATGGACCATTTCAAAAGAATTTAATTGATACAATGCTTAGACTTCGTTTTGCAGGTATTAAAGATGCTGCTATGGAAATATTAATTAAAGCCAATGCTTTTAGAGACTTTGGACACATGAAATATATACTATCTTGTGATAATCAAATTAAAGACATTTTTAAGGTTTTATCTTCACATTCAACTTATAAAGAAGCATTAAAAGATATTCAAAAAATGGGTTACTATGATATAGTTTTTGATCAAGAAAATGAACGTGACATAAATTACGAAATGGACTATGAAATAGCTCTTTTAGGTGAATTATACAATGCTTTTAGTACTTCAATTTATGAAGCAAAATACAAAAATAAATTAAATACAATAACCTCTTCAATTCCTGTTTCTGTTGCTGCTGAAGTAGCTGATTTAAGAAGACCAAGAGGTAAAGAATTTAGCGTTTTAGAATTAAGAGATAGTACAGCTAAAATTACTTTCTTTGTTAACAACAAAGTATTGGCAAGCTCACTTCCTCTTAAAAAAGGACAAATAATTGAAGCTACAATTAGTTTCAGTCGCAACAAACATAATTTAATTAGCTGA", "aa": "MAIRDMGRVMGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKNDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIQKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLIS", "aa_hexdigest": "214f59e33a5be4b722ab07b28a5be231", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00244ECC9B", "query_cov": 0.8768971332209107, "subject_cov": 0.9683426443202979, "identity": 0.5, "score": 482.0, "evalue": 4.67e-162, "valid": false, "uniref50_id": "UniRef50_UPI00244ECC9B" }, "pscc": { "uniref50_id": "UniRef50_UPI00244ECC9B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244ECC9B" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 922107, "stop": 924203, "observations": { "mutation": [ 922124, 924173 ], "stop": [ 922124, 924173 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF83", "query_cov": 1.0, "subject_cov": 0.6132368148914168, "identity": 1.0, "score": 1156.0, "evalue": 0.0, "gene_start": 1, "gene_end": 593, "reference_start": 370, "reference_end": 962, "reference_sequence": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGAFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKAIEISELWNPNLIVLDHPRYGYYAQTKDNKYSASKNYYINSSDASLPNSILIKENKLFEASDNETLNYLQKLGNLEETKHYANYFDEVNVDSEIIDRIYSIINECNVIFPKKALHLAPFQNNDEATNEKLFVSLLKAGAKRLASELKNYPDWNKRLEYEFNVIKKLGYINYFLIIQDLVNWAKSQKIAIGPGRGSAAGSLVSYLLNITEINPLRYNLLFERFLNPDRVSWPDIDIDIQDDRRFEVFEYLQKKYGVDKTGLISTFQTIGAKMAIRDMGRVMGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKNDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIQKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLISWKGIQ" }, "paralog": false, "identity": 0.997134670487106, "score": 1367.0, "evalue": 0.0, "description": "Internal stop codon at 922124, 924173. Nonsense mutation around 922124, 924173.", "psc": { "uniref90_id": "UniRef90_C4XF83", "product": "DNA-directed DNA polymerase", "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF83", "UniRef:UniRef50_D3VPK4" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4" ], "product": "DNA-directed DNA polymerase" } }, "id": "EFPKPENHBP_1690", "locus": "GCA000186005_01681" }, { "type": "cds", "sequence": "contig_1", "start": 924690, "stop": 925010, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATACGTCAATTTATACAATACAAGTGAATACACTTTTTTAGATTCTTTAATTAGAATAGATGATTTAGTTAGATTATCAAAAGAACACAATCTTCCTGCAGTAGTTTTAAGTGACCGAAACAATATGTTCGGTTTAGGTGCTTTTATGGAAGCATGCTTAAAATATGATATCAAAGGTATCATTGGAGTGGACTTAGATGTAGACAATTATCGCTTCATAGTCTTAGCGAAAAACTACCAAGGTTACCAAAAAATTAATCAACTAATTTATCAAAAAAGTATTAATAAAGCTATTGAAATTAGTGAATTATGA", "aa": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGAFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKAIEISEL", "aa_hexdigest": "9686477b1aa1f35787b12f70e3cf0973", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12302.101399999989, "isoelectric_point": 6.556229972839356 }, "id": "EFPKPENHBP_1691", "locus": "GCA000186005_01682" }, { "type": "cds", "sequence": "contig_1", "start": 925114, "stop": 925368, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82", "UniRef:UniRef90_C4XF82" ], "nt": "ATGGCAAAAAAGATGAGTAAAGGTGCTATTATTGGTATATCAGTAGGAGCTACAATTGTAGTAGCTAGCACAGTAACCGCTGCTCTTGTGTGTACATTGCATGGAAAAACTGTTTATAAAATTGAATACTCTAAGTATAAAAGAGCTAGAGAAAAAGGCAATATTGGAGACAAAATAGCTTTAGCTACTTTAGAAGGACTTATTGTAACAGGTGAAACAACTTTGAAAGAAATAAATGGAACAAACCAACAATGA", "aa": "MAKKMSKGAIIGISVGATIVVASTVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQ", "aa_hexdigest": "c4fb286dcbf1bd2d53deda2be696dcb0", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 925054, "stop": 925494, "observations": { "mutation": [ 925366 ], "stop": [ 925366 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF82", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 0.988, "score": 155.0, "evalue": 2.94e-47, "gene_start": 1, "gene_end": 84, "reference_start": 21, "reference_end": 104, "reference_sequence": "MFLYLVVKIVKLLYTFRRCIMAKKMSKGAIIGISVGATILVASTVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQWLDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK" }, "paralog": false, "identity": 0.9794520547945206, "score": 276.0, "evalue": 4.82e-97, "description": "Internal stop codon at 925366. Nonsense mutation around 925366.", "psc": { "uniref90_id": "UniRef90_C4XF82", "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF82", "UniRef:UniRef50_C4XF82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8867.319099999995, "isoelectric_point": 9.673819541931152 }, "id": "EFPKPENHBP_1692", "locus": "GCA000186005_01683" }, { "type": "cds", "sequence": "contig_1", "start": 925369, "stop": 925494, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAGCTAAATTTGTAAAAGACACTAGAAAAGAAATAATAGGAAATAAATCAAAAGCTGATTTTCAAAAATTAATTGATGATTTTAAAAATTTTTTAAAAAGTTTAAAAGTAAAAAAATAA", "aa": "MDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK", "aa_hexdigest": "0157d037a33d4ab7c75ab6e9e8358ce0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4829.7037, "isoelectric_point": 9.919701957702635 }, "id": "EFPKPENHBP_1693", "locus": "GCA000186005_01684" }, { "type": "cds", "sequence": "contig_1", "start": 925526, "stop": 925990, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGCAGCTTTTTATGATCGTAGTGAATTCTTAAATTATTCAATTAGAATGCTTGATATTTATGAAGGAACAAAACTCAAAAACATTTTTACAGCTTTGGAAAACAATGCAAAATTTGGTTTAAAATCACTTAACCTATTTTACGAAAAGGTCATTAAAAAAGTTGTTTTCGACAAAAAAATAGTTGATTTATCACAATCAATTAAAAACACATTATTAGGTCGTGAACTAATACTTAATGAGTATTTTTCATCAACATCACTTAAATCATTTGACAATCATGCAATGTTTTTAAAATTTAAAGACGCAAAAGAAATGAATAAATATTTAAAGAAAATTGAACCAACTAAAATTCATTTTGAAACAAGCAAAGTATTCTTAAATTATCTTTCTCCAAAGGTAAATACAAACAAAGTTGACATTCGTTTTGTTTTAACTAAATTAGTTAACAATTATGTCTAA", "aa": "MFAAFYDRSEFLNYSIRMLDIYEGTKLKNIFTALENNAKFGLKSLNLFYEKVIKKVVFDKKIVDLSQSIKNTLLGRELILNEYFSSTSLKSFDNHAMFLKFKDAKEMNKYLKKIEPTKIHFETSKVFLNYLSPKVNTNKVDIRFVLTKLVNNYV", "aa_hexdigest": "bf7090c90e3190d404d3e8e6769c7931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18152.047100000003, "isoelectric_point": 9.631077003479003 }, "id": "EFPKPENHBP_1694", "locus": "GCA000186005_01685" }, { "type": "cds", "sequence": "contig_1", "start": 926082, "stop": 926321, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAATAGAATCACTAAGTGTTTCTTCATTATTTACAAATTGTAATAATTTGAAAATGCTTGATGACATTAGTCAATATCTATTAGGTATATTTACACCACGATATTTTTCAACTAAACTAGTTGGTATTAATTTTGCATATTCAATTTCAACTTGGTTATTGTTAATTTGCCCCACAAAAAGATTTTCTTTGTTTATTATTGCTTTATATTCTTTAATTTTGAAAACATGA", "aa": "MKYKIESLSVSSLFTNCNNLKMLDDISQYLLGIFTPRYFSTKLVGINFAYSISTWLLLICPTKRFSLFIIALYSLILKT", "aa_hexdigest": "8b269059cf18d5face1a4ee872d2143d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9125.817399999989, "isoelectric_point": 9.469454765319824 }, "id": "EFPKPENHBP_1695", "locus": "GCA000186005_01686" }, { "type": "cds", "sequence": "contig_1", "start": 926390, "stop": 926542, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAATTTAAAAGTTAATCTTAAATTAAAAGAATATAACGAATTAAAAAGATTAATATTTGATTTAGACACAAATATGAAAGAAACTATCCAATCAGTTACTAATAAAGAAAAGATTACATTATTAGATTCAACAGCTTTAATGTGA", "aa": "MLNNLKVNLKLKEYNELKRLIFDLDTNMKETIQSVTNKEKITLLDSTALM", "aa_hexdigest": "cfe4bc8845d516f8858587124dd4f538", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5869.8679, "isoelectric_point": 8.034904289245606 }, "id": "EFPKPENHBP_1696", "locus": "GCA000186005_01687" }, { "type": "cds", "sequence": "contig_1", "start": 926708, "stop": 926863, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTATTAAGAAACTTAAACCATATTTTGAAAAATACAAAAGATCACAAGACAAAATTCATAATTTGATGCTATTAGATTGGTTGATAGCTCTTCTTTTAGTTTTATCATCTGTTATTATGGGAGTTTTACTTCATTATTTAAATAATTGA", "aa": "MLFIKKLKPYFEKYKRSQDKIHNLMLLDWLIALLLVLSSVIMGVLLHYLNN", "aa_hexdigest": "c7b85156445c61eadc330b4514ac14f8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6118.452799999997, "isoelectric_point": 9.698381996154787 }, "id": "EFPKPENHBP_1697", "locus": "GCA000186005_01688" }, { "type": "cds", "sequence": "contig_1", "start": 926963, "stop": 927514, "strand": "-", "frame": 3, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "GO:0006412", "GO:0008135", "RefSeq:WP_013354916.1", "SO:0001217", "UniParc:UPI0001A8C2B1", "UniRef:UniRef100_C4XF80", "UniRef:UniRef50_Q98QZ1", "UniRef:UniRef90_C4XF80" ], "nt": "ATGGAATTAGATTTATATTTATTAGATTTTTTAGATGAGGCTGAAAAAGCAATTAATCATTATGCTTTTGAATTATCAAAAATTTCAACTGGACGTGCTAACCCTCAATTAATTAAGGGAGTTAGAATTAATTATTATGATACTTTGACACCTCTTGAAGAACTTGCTTCAATTACAGTACCAGAACCACAACAATTATTAATTAAACCTTTTGACGTTTCATGCAACCATGAAATTGCAAAAACAATTTTAAATGCTAACTTAGGTTTAAATCCTGTTGATGAAGGAAATCAAGTTAGAATAACTTTTCCTCCTCTTACAACTGAAAGACGTAAAGATATGGTTAAGAGTCTTGCAAAATTTACAGAAAATGCTAAAGTTGCAATTAGAAATGCAAGACAAAATGTTAACAAATCTATCAAGGCTGATGAAGAATTATCAGAAGATCAAGAAAAGAAATACTTAGATGAAATTCAAAAAGAAGTTGATAAAAAAATCGAACACATTAACAAAATGACAATCAACAAAGAAAAAGAATTAATGAGCATATAA", "aa": "MELDLYLLDFLDEAEKAINHYAFELSKISTGRANPQLIKGVRINYYDTLTPLEELASITVPEPQQLLIKPFDVSCNHEIAKTILNANLGLNPVDEGNQVRITFPPLTTERRKDMVKSLAKFTENAKVAIRNARQNVNKSIKADEELSEDQEKKYLDEIQKEVDKKIEHINKMTINKEKELMSI", "aa_hexdigest": "256d7dc815b02298fa3ae94b2c0cbff1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C2B1", "ncbi_nrp_id": "WP_013354916.1", "uniref100_id": "UniRef100_C4XF80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2B1", "RefSeq:WP_013354916.1", "UniRef:UniRef100_C4XF80" ] }, "ips": { "uniref100_id": "UniRef100_C4XF80", "uniref90_id": "UniRef90_C4XF80" }, "psc": { "uniref90_id": "UniRef90_C4XF80", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_Q98QZ1", "go_ids": [ "GO:0006412", "GO:0008135" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QZ1" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "EFPKPENHBP_1698", "locus": "GCA000186005_01689" }, { "type": "cds", "sequence": "contig_1", "start": 927514, "stop": 928005, "strand": "-", "frame": 1, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGTTGGCAACTATCATGAATGGTCTAGCTCTTAAAAGTGGATTCGAAAAAGTTGGATTAAAAACTAGAATCCAATCATCAATTGCAATTGATCAAAAAGTTGCAGAAAATTATATTAATGAAAAGACTTTAAAATACCTTGAAGAAGGTGAAGTAGTTATTTTTGTTGGCGGTACAGGCCGTCCTTACTTTACAACAGATACAGCAGCAACATTATTTGCTTCAGAAATCAAAGCCGAAGTTATCTTAATGGGTAAAAATGGTGTTAGTGGAATCTATGATTCAGACCCTAAGAAAAATCCAAATGCCAAGAAATATGATGTTGTAACTTATGATGAAATTTTAGACAAAAAATTACAAGTCATGGACTTAACGGCCACAAGTATGGCTAGAGACAACAATATTAATTTAATTGTTTTCAACATTAAAGAAAAGAATGCTATTGTCAAAGCTCTTGAAGGTAAAATTGAACACACAGAGGTAATTAAATAA", "aa": "MLATIMNGLALKSGFEKVGLKTRIQSSIAIDQKVAENYINEKTLKYLEEGEVVIFVGGTGRPYFTTDTAATLFASEIKAEVILMGKNGVSGIYDSDPKKNPNAKKYDVVTYDEILDKKLQVMDLTATSMARDNNINLIVFNIKEKNAIVKALEGKIEHTEVIK", "aa_hexdigest": "04f05b835647ab07dc625ab87faab339", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 1.0, "subject_cov": 0.9588235294117647, "identity": 0.595, "score": 204.0, "evalue": 7.06e-65, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "EFPKPENHBP_1699", "locus": "GCA000186005_01690" }, { "type": "cds", "sequence": "contig_1", "start": 928063, "stop": 928230, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATACAAACGTATATTAGTAAAACTTTCAGGTGAAGGTTTAGTAAATAAACAAAAAAGTTTAGCTATTGACTATGATTTAGTAGAAGACATTGCTAAACAGCTTAAAAAAATTATTGACAAAGGTGTTCAAGTTAGTGTTGTTATTGGTGGAGGTAACTTTTGA", "aa": "MKYKRILVKLSGEGLVNKQKSLAIDYDLVEDIAKQLKKIIDKGVQVSVVIGGGNF", "aa_hexdigest": "467ce7109c83ee61f555fab9336f2359", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.142000000002, "isoelectric_point": 9.573829078674315 }, "id": "EFPKPENHBP_1700", "locus": "GCA000186005_01691" }, { "type": "cds", "sequence": "contig_1", "start": 929090, "stop": 929491, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAGAAAAGAAACATTTTTCGAACGTTTGACAAGAAAAAATGCCGAACACGAATATAAAAATCAACAAGCAATGCCTAAAAAGAAAAAGCTAAAAGGTATAATCCTTGGAACACTTTTAACATCTGCAATTGCAACAGGTATCATTGTTCCAGTTGTAATTAATTCAACAAAAGTAACATATAAAGACGCACTCAAAGATGAAAGTGCTGTTTTAACATTTACATTACCAGGATCAAAAGAAAAACATTCTATTACAATCAAAGAATTAAAAAATTCAAGTAAAACTGAAGATGCTAAAGATCCAAGTAAATTGTTACAACAAATTAAACGTCTTGCGATGTTTTATCTTTATGATAAAGAAGCAAAAGCCTCAGCTGAATATCAAAGATTATGA", "aa": "MAKRKETFFERLTRKNAEHEYKNQQAMPKKKKLKGIILGTLLTSAIATGIIVPVVINSTKVTYKDALKDESAVLTFTLPGSKEKHSITIKELKNSSKTEDAKDPSKLLQQIKRLAMFYLYDKEAKASAEYQRL", "aa_hexdigest": "52ed278054ab1d2b5f2f7733a813549f", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15118.514799999979, "isoelectric_point": 9.835119438171386 }, "id": "EFPKPENHBP_1701", "locus": "GCA000186005_01692" }, { "type": "cds", "sequence": "contig_1", "start": 929495, "stop": 929629, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACGTGAAGGCAATGAAGAAGAAAATACTTCATTCAAATTAAAATCAATTGAAGAACTTAAAACAAAAGAAAGAAACAAGATTCTAGATATTCAAGAAAACACCAAAAGAACAGCCGGTTATGATAAATGA", "aa": "MSREGNEEENTSFKLKSIEELKTKERNKILDIQENTKRTAGYDK", "aa_hexdigest": "11e3adff4aa866077e77563db5e5371f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5202.721500000002, "isoelectric_point": 6.228837394714356 }, "id": "EFPKPENHBP_1702", "locus": "GCA000186005_01693" }, { "type": "cds", "sequence": "contig_1", "start": 929918, "stop": 930079, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTACAACAAATAGTTATGTTAAAGCATATAAAGATGCATCTGCATTTATTGATGAATACTATAAAAATGATAATCCATATGCATTCACACAATTTACGATTCCAGGTGTTGCACCTACAAAAATAGCTAAAAAAGGCGAAAAAGAATTAGAATGA", "aa": "MSFTTNSYVKAYKDASAFIDEYYKNDNPYAFTQFTIPGVAPTKIAKKGEKELE", "aa_hexdigest": "10cb30245bb335ec7585f92502734142", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6032.693200000001, "isoelectric_point": 6.175977134704589 }, "id": "EFPKPENHBP_1703", "locus": "GCA000186005_01694" }, { "type": "cds", "sequence": "contig_1", "start": 930386, "stop": 930697, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAATTAGTTTATGGATCAACCTCTGTTCCCGAAATTGATTTAAAAGCATTATTTGAAAGTATTCAATCTAAATTAGAAACACTATTCCCTGAATTAGTTCCATTAAAACCAAATTATGATGCTACAGCTTCATTAGAAGAAAAAACTAAAAAAGTTGGAGATTACAACAAAAAACTTCTTGACATCATGAGAGATTTTGATAACAACAAGAAGAGTGGTTTATATGATGACAAATTAAAAGAAGAAATCATACCACTAATTGCTAAAGCTTTTGAAGGTGCTGACAAAAAAATAAGAACATTCTGA", "aa": "MPELVYGSTSVPEIDLKALFESIQSKLETLFPELVPLKPNYDATASLEEKTKKVGDYNKKLLDIMRDFDNNKKSGLYDDKLKEEIIPLIAKAFEGADKKIRTF", "aa_hexdigest": "568dd8127204cced78cae8887d949be6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11740.414299999984, "isoelectric_point": 5.226254463195799 }, "id": "EFPKPENHBP_1704", "locus": "GCA000186005_01695" }, { "type": "cds", "sequence": "contig_1", "start": 930806, "stop": 931129, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAAATCTTAAAAAATGATTTTATTTTAAGCAAAAAATACAAATCAATTAATGCAACAAAATACAATGCATTAGATGTAATTTCTAAAAACTTAAAATCAGATTATTATGTAAATAAAGTTTTATTAGAAGATGCTGATTTTGTTAAATATCTCAAAGAACAAGAAAATATTTATGCACTTGATGCACAAGGCAAAGCAGTAAAAGGTGTTAAATATTCTGACAAAGATATTGAAGAATTGAAAAAATCGAATGAAATTAAATACAGAATTGATGCTGAACAAAACGCTCTGGATGTTAATAAGAAATTAACAACTTGA", "aa": "MLEILKNDFILSKKYKSINATKYNALDVISKNLKSDYYVNKVLLEDADFVKYLKEQENIYALDAQGKAVKGVKYSDKDIEELKKSNEIKYRIDAEQNALDVNKKLTT", "aa_hexdigest": "6d17360b7967e11d7914c9e665b67152", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12401.071199999984, "isoelectric_point": 8.596230506896976 }, "id": "EFPKPENHBP_1705", "locus": "GCA000186005_01696" }, { "type": "cds", "sequence": "contig_1", "start": 931298, "stop": 931462, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAATTATTATTTTCTTTAGGAAGCATTAGCACTTTAGCAGCCTTCCCTATAGCCGCTGTTAGTTGTGGTCGTGTTGTTGAAACTCCAGCTAAAAAAATTCAAGATGATTTCTTAAAAGCTGGCGGCAGAAAAGAAGAAATCAAAAACATTTGA", "aa": "MKNKKLLFSLGSISTLAAFPIAAVSCGRVVETPAKKIQDDFLKAGGRKEEIKNI", "aa_hexdigest": "f1d824eabbbe97e3b76ca09f2e1daa01", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.865599999999, "isoelectric_point": 9.806624412536618 }, "id": "EFPKPENHBP_1706", "locus": "GCA000186005_01697" }, { "type": "cds", "sequence": "contig_1", "start": 932054, "stop": 932182, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTTCGGAAGATGAAAGTAAAAAAATAAGTGACATTGAAAAAATTACTGATAATGCATACGAAAGTCAATTATTAGGCTATCAAGGTATTAACTTAAACTTCAGTAATTACAACTTGAAATGA", "aa": "MKGSEDESKKISDIEKITDNAYESQLLGYQGINLNFSNYNLK", "aa_hexdigest": "28c62bf59b566fa09c777662ec10cfd6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4799.2388, "isoelectric_point": 4.715101432800293 }, "id": "EFPKPENHBP_1707", "locus": "GCA000186005_01698" }, { "type": "cds", "sequence": "contig_1", "start": 932643, "stop": 932978, "strand": "+", "frame": 3, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "RefSeq:WP_013527111.1", "SO:0001217", "UniParc:UPI0001A8C2AC", "UniRef:UniRef100_C4XF75", "UniRef:UniRef50_A0A4P1QFY6", "UniRef:UniRef90_C4XF75" ], "nt": "ATGCAAAACATATTTCAATTAATAATTAACAAAAAAGCAGAAGCAAATATTATCTTTGAAGATGATAAATGTATAGCTTTTTATGACAAATTCCCAATTCAACCCGGACATTTCTTAGTGGTTCCAAAAAAACACAGTAAAAACATTACTGAAGCAGATGATGAAACAGCTGCACATTTAATAAATGTTGCTCGTAAACTTGGAAAAAAACATGTATTAGATAAGAAAATTGCAGGCTTCAAAATAATAATTAATACAGGCGAAGCTGCTGATCAAACAATTTTCCATACACACGTACACGTAATTCCATATCGTGAAAAAGCACAAGATAACTAA", "aa": "MQNIFQLIINKKAEANIIFEDDKCIAFYDKFPIQPGHFLVVPKKHSKNITEADDETAAHLINVARKLGKKHVLDKKIAGFKIIINTGEAADQTIFHTHVHVIPYREKAQDN", "aa_hexdigest": "ae4ed3bd0dadeae14978ab7253fc607f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C2AC", "ncbi_nrp_id": "WP_013527111.1", "uniref100_id": "UniRef100_C4XF75", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2AC", "RefSeq:WP_013527111.1", "UniRef:UniRef100_C4XF75" ] }, "ips": { "uniref100_id": "UniRef100_C4XF75", "uniref90_id": "UniRef90_C4XF75" }, "psc": { "uniref90_id": "UniRef90_C4XF75", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_A0A4P1QFY6" }, "pscc": { "uniref50_id": "UniRef50_A0A4P1QFY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P1QFY6" ], "product": "HIT domain-containing protein" }, "genes": [ "hinT" ], "id": "EFPKPENHBP_1708", "locus": "GCA000186005_01699" }, { "type": "cds", "sequence": "contig_1", "start": 933173, "stop": 933448, "strand": "+", "frame": 2, "gene": null, "product": "DNA-binding protein HU", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74", "UniRef:UniRef50_A0A0D5ZK80", "UniRef:UniRef90_C4XF74" ], "nt": "ATGACCAAAAAAGAATTTGTACAAAAAATTGCTGAAATGTTAGAAGTTCCAGTTAGAGTAGCAGATCAATACTTTGATGCATTTCTATTCGTTTTAAAAGAACAACTTATAGCTGAAGATAAAGTTCAACTTTCAGATTTAGGAACCTTCGAAACAAAAATTAGAAGAGGACGTGAAACAATTAATCCATTTACAAATGAACCAATTCAAGTGCCTGAAAAACGTGTTGTAAAATTCACACCTTCAAAATACTTAAGAGATATTGTAAACTTCTAA", "aa": "MTKKEFVQKIAEMLEVPVRVADQYFDAFLFVLKEQLIAEDKVQLSDLGTFETKIRRGRETINPFTNEPIQVPEKRVVKFTPSKYLRDIVNF", "aa_hexdigest": "752c415395f60aa5b520b05f65e60a9d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32FB7", "uniref100_id": "UniRef100_C4XF74", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74" ] }, "ips": { "uniref100_id": "UniRef100_C4XF74", "uniref90_id": "UniRef90_C4XF74" }, "psc": { "uniref90_id": "UniRef90_C4XF74", "product": "DNA-binding protein HU", "uniref50_id": "UniRef50_A0A0D5ZK80" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZK80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZK80" ], "product": "Viral histone-like protein" }, "genes": [], "id": "EFPKPENHBP_1709", "locus": "GCA000186005_01700" }, { "type": "cds", "sequence": "contig_1", "start": 933461, "stop": 933949, "strand": "-", "frame": 3, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "EC:3.1.21.10", "KEGG:K03700", "RefSeq:WP_013527112.1", "SO:0001217", "UniParc:UPI0001F598D2", "UniRef:UniRef100_A0AB32XCN2", "UniRef:UniRef50_F9UJF5", "UniRef:UniRef90_A0A3P8LHU8" ], "nt": "ATGCAAAAAAATAGAGGAATGTTATTAGAAAAAATAATTAATAAAACAATTGATTACTATGCCAAAAATAAATTAGCTTATATTGAAAAGAAGTCTTTACCTGTTAAGTTTAAGAAGGTTGAAAATGATAATTTGAAATTGGATGAGGCTTATATATTAAAGAAGAGCACAGTTGATTACATCGGTTGCTATAAAGGATTTTTTGTTGCTTTTGAAGCAAAAAGCACAAATGAAAAAAGATTGCCAGCAAATAATATTGCTCGGCACCAAATTAAGTATTTATTGGATATTGAAAGTAATAGTGGATTAGCTTTCTTTATTATATTTTTTAGTTTTTGTGATGAGTTTTATCTTGTTAAAGCAAGTGAGTTAATTCAGCATATGAATAAATCTATTTCTTATGAAGAAATAAAAAACATAGGTTTTAGAATCGAATTAACCTTTCCAGGTATAATCGACTTTTTGCCCTATGTTCAAAATTTTTATTAG", "aa": "MQKNRGMLLEKIINKTIDYYAKNKLAYIEKKSLPVKFKKVENDNLKLDEAYILKKSTVDYIGCYKGFFVAFEAKSTNEKRLPANNIARHQIKYLLDIESNSGLAFFIIFFSFCDEFYLVKASELIQHMNKSISYEEIKNIGFRIELTFPGIIDFLPYVQNFY", "aa_hexdigest": "50c3e1e079c072e9b7eef0e033bbbc0e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F598D2", "ncbi_nrp_id": "WP_013527112.1", "uniref100_id": "UniRef100_A0AB32XCN2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598D2", "RefSeq:WP_013527112.1", "UniRef:UniRef100_A0AB32XCN2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCN2", "uniref90_id": "UniRef90_A0A3P8LHU8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LHU8", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_F9UJF5", "kegg_orthology_id": "K03700" }, "pscc": { "uniref50_id": "UniRef50_F9UJF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UJF5" ], "product": "Holliday junction resolvase RecU" }, "genes": [ "recU" ], "id": "EFPKPENHBP_1710", "locus": "GCA000186005_01701" }, { "type": "cds", "sequence": "contig_1", "start": 934086, "stop": 934424, "strand": "+", "frame": 3, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63", "UniRef:UniRef90_C4XF72" ], "nt": "ATGAAATATAGAAAGATAATATTAGGAACTTTGTTAGCAGGCACAATTGTAATTTCAACAAGTACAATTGCTTTTAATATTCAAAGAACCAAGGTTGCAAAGGTTAAAAGCAATGATCCTGCATCAATAGGATCAAAATTTTGTATCAAAGTATCCGGTGCAGTTGCCTTTGCTAAAAATTATTATTTCGAAAAGCCTATTTATTTAAAAGAATTATTAGATATGGCTCAACCTTATGAAAATGCAGACTTAAGCAAAATTGACTTAAAAAAAGAATTAAAAGAAAATCTTAATATTTACATTCCATATCAAAAAACTAATAACCCTTTTTTGTATTGA", "aa": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKIDLKKELKENLNIYIPYQKTNNPFLY", "aa_hexdigest": "b4bb191edb56bd4a657b021e31468864", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 934086, "stop": 934604, "observations": { "mutation": [ 934422 ], "stop": [ 934422 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF72", "query_cov": 1.0, "subject_cov": 0.6511627906976745, "identity": 1.0, "score": 218.0, "evalue": 3.23e-71, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKIDLKKELKENLNIYIPYQKTNNPFLYWNNLSKEHFKLLVDFGIKKSIVNKILDLREKKNQITWNDLENISGVGKVTLKKLQGILIL" }, "paralog": false, "identity": 0.9941860465116279, "score": 335.0, "evalue": 1.81e-119, "description": "Internal stop codon at 934422. Nonsense mutation around 934422.", "psc": { "uniref90_id": "UniRef90_C4XF72", "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF72", "UniRef:UniRef50_A0A3P8MF63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63" ], "product": "Competence protein ComEA" } }, "id": "EFPKPENHBP_1711", "locus": "GCA000186005_01702" }, { "type": "cds", "sequence": "contig_1", "start": 934751, "stop": 935134, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTGACTTTATATTTAAGTTATTTTTTATCTAAAACAAAAATTTATTATGAAAAAGGTATTTATAAAATTGAAGGATCGATAAGCGTCCTAAGCAAAAAATATTTAGTTATATCAAATGAAAATAATAATATTTTAATTTACACAAGTGATTTAAAAAATAGCACTCCTTTAAGTCTAGGGGACCAAGTAGAAATTCAAGGTGAATTAAATCCTATTGAAATAGACAATAGTAAAATAGCAACATTTTATTTGCAAAATAATATAAATTATTTGCTAAAAAAAACAGTCATTTTAACTGTTATTAAACCAGAATTTTCTGTTGAAGAAAAAATAATGAATTATTCAAAAAATCATGGTGAATTATTTGATTCATATTGA", "aa": "MLLTLYLSYFLSKTKIYYEKGIYKIEGSISVLSKKYLVISNENNNILIYTSDLKNSTPLSLGDQVEIQGELNPIEIDNSKIATFYLQNNINYLLKKTVILTVIKPEFSVEEKIMNYSKNHGELFDSY", "aa_hexdigest": "61abfd4da6f33bf809c7d9d8be2e1711", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14675.736699999985, "isoelectric_point": 5.824996376037597 }, "id": "EFPKPENHBP_1712", "locus": "GCA000186005_01703" }, { "type": "cds", "sequence": "contig_1", "start": 935138, "stop": 935656, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTTTTGGATCCTATGATTCAAAAGAATTAGTATTGAATAAAGTTAATAGACTTAATATTGTTCATTTAATTGTTATTTCAGGTTTGCATTTTGACTTATTATTCTATTTAATTTTGTTGATACTAAAACCTTTGGATAAAATTAAAATCAAAAGTAAATATGTGGCTTTTGCCCTCATATTCTATTATTTAACATTACTTGCTAATCCTGTTTCTGCTTTAAGAGCTTATATTATGCAAGTTGTTAAAAATTCAAATAATCCTTTTAGTTATAACAACAAATTTAAAAAATATGATGGTCTAGTAATTTCAATTTTAGTTTTATTTTTACTTAAAAATAATTACTTGTTTTCTTTATCATTTATTCTTAGTTTTTCATCAACTTTTACAATTATGTTATTAGTGCCAATGGTCAAAAGTAAAAAATTAAACAACTTTTTAAAAACATTAATTTTGATGACTTTAATATATCTTTTTAATATTCCAATGCTTTTATATATTAATGAATATTGA", "aa": "MLVFGSYDSKELVLNKVNRLNIVHLIVISGLHFDLLFYLILLILKPLDKIKIKSKYVAFALIFYYLTLLANPVSALRAYIMQVVKNSNNPFSYNNKFKKYDGLVISILVLFLLKNNYLFSLSFILSFSSTFTIMLLVPMVKSKKLNNFLKTLILMTLIYLFNIPMLLYINEY", "aa_hexdigest": "28fa8e154319eeb45cba1d1d4202ac9a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20042.13240000001, "isoelectric_point": 9.810234642028806 }, "id": "EFPKPENHBP_1713", "locus": "GCA000186005_01704" }, { "type": "tRNA", "sequence": "contig_1", "start": 935962, "stop": 936038, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 69.4, "nt": "GTACCCATAGCTCAACTGGACAGAGTGTCAGGTTACGGCCCTGAAGGTTGCGGGTTCGACTCCTGCTGGGTACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 936002, 936004 ], "id": "EFPKPENHBP_19", "locus": "GCA000186005_01705" }, { "type": "tRNA", "sequence": "contig_1", "start": 936043, "stop": 936117, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 69.0, "nt": "GACACCATAGCCAAATGGCCAAGGCATGAGTCTGCAAAACTCTGATTACGGGTTCGAATCCCGTTGGTGTCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 936092, 936094 ], "id": "EFPKPENHBP_18", "locus": "GCA000186005_01706" }, { "type": "cds", "sequence": "contig_1", "start": 936362, "stop": 937168, "strand": "-", "frame": 3, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2", "UniRef:UniRef90_C4XF70" ], "nt": "TTGGACTTAAATGAAGCTTTAAAAGAATTTGATTATTTAATTTTAGCTGTACCTTCAAGTGCAATTACTTCTGTGTTAGAACAAATAAAGGAAACAATCAAAAATAAAAAAATAAATGTTATTAATGTTGCAAAAGGTATTGATGAAAAAACAAATGAATTTTTTTCAGAAGTTTTAAACAAACAATTTGCGAATAATTTAAAAAATTATTGTACTTTAATTGGTCCTTCTTTTGCTGTTGAAGTTTTTGAAAATGTTTTAACCATGATTAATGTTGTTGGTCCAAAAACAAGATTTTTAAAAAAAGTTTCTGAATTATTTAATAATGACACTTTTAGACTTGTTGTTAATGAAAATGAACACGGTTCAGAATTGTTTGCTGCTCTAAAAAATGTTCTAGCTATTGGAATTGGAATGATTGATTATTTAAGACCATATCGAAATACAATGGCGGCCTTGCTTTCAATTGGTGTTAAAGAAATTCACATGGTTTACAAGAGAATATTTCCTGAATCAAATGACAATATGGGATTTGAATTAGCAGGAATTGGCGATATCTTTTTAACTTGTTCAAGTACAAAAAGCCGTAACTTTTCTTTTGGTAGACAAGTTGCAGAATTTGGCTTGGAAGCAACTTTAAAAAATAATAAGAAAACTATTGAAGGATATCATGCTGCTAAAATATTAGCTAATATTCTAAAGAAAAATAAAGATCTTAAAATTGTATTTTTAAAAAGCATAATTGATATTTTATATAAAAACAAGAAACCTGAATTACTTTTAGATTTTGTTAAAAAATATAACTAA", "aa": "MDLNEALKEFDYLILAVPSSAITSVLEQIKETIKNKKINVINVAKGIDEKTNEFFSEVLNKQFANNLKNYCTLIGPSFAVEVFENVLTMINVVGPKTRFLKKVSELFNNDTFRLVVNENEHGSELFAALKNVLAIGIGMIDYLRPYRNTMAALLSIGVKEIHMVYKRIFPESNDNMGFELAGIGDIFLTCSSTKSRNFSFGRQVAEFGLEATLKNNKKTIEGYHAAKILANILKKNKDLKIVFLKSIIDILYKNKKPELLLDFVKKYN", "aa_hexdigest": "c28994641dc0ad58ef5021e2f010c03b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF70", "query_cov": 1.0, "subject_cov": 0.8072289156626506, "identity": 0.996, "score": 514.0, "evalue": 5.07e-183, "valid": true, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A168RLR2" }, "pscc": { "uniref50_id": "UniRef50_A0A168RLR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "EFPKPENHBP_1714", "locus": "GCA000186005_01707" }, { "type": "cds", "sequence": "contig_1", "start": 937370, "stop": 937522, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCCATCAAGTATTATGAAAGTGATCAAAAAGAAAAAGCTATTAAATACTATAAAGCATATTGTACAATTGCTTTTAAACGCAAAAAAGATTACAGTGATAAAAATTCTAATAACAAAAATGATGATGACACAATAATAGTTAAATAA", "aa": "MKAIKYYESDQKEKAIKYYKAYCTIAFKRKKDYSDKNSNNKNDDDTIIVK", "aa_hexdigest": "238be7c57edffd201e402de07e650815", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6018.782800000004, "isoelectric_point": 9.352766990661618 }, "id": "EFPKPENHBP_1715", "locus": "GCA000186005_01708" }, { "type": "cds", "sequence": "contig_1", "start": 937646, "stop": 937795, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTAGATTTAGAATATGAAAAAGAATTAATTGTTGTTTGTAAAAACTATCATGAAGAAGCACAAGCTTATAAAAATGCCAAAAAGTTCATAATTATTTTAGTAACGCTTTGCTATCTTATTGCTTTTGGACTTTTTGCGGTTTGA", "aa": "MDLDLEYEKELIVVCKNYHEEAQAYKNAKKFIIILVTLCYLIAFGLFAV", "aa_hexdigest": "20f9c44fba52834201648f7fe98c8b53", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5727.751199999997, "isoelectric_point": 5.090181922912596 }, "id": "EFPKPENHBP_1716", "locus": "GCA000186005_01709" }, { "type": "cds", "sequence": "contig_1", "start": 937803, "stop": 938579, "strand": "-", "frame": 2, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "EC:2.1.1.182", "GO:0000179", "GO:0006364", "RefSeq:WP_013527117.1", "SO:0001217", "UniParc:UPI0001A8C2A5", "UniRef:UniRef100_C4XF68", "UniRef:UniRef50_N9V0N0", "UniRef:UniRef90_C4XF68" ], "nt": "ATGTTTCAAGAAAATAAATTAAAAGCCAAAAAAAGATATGGTCAAAATTTTTTAAAAGATCAAAATGTTATTAACAAAATAATTGAATTGATTAAACCACAAGGTGAAAAGATTTTAGAAATAGGCCCCGGAAGAGGTGCTTTAACTAAAATTTTAAATCAACAAGCAAAAGAATTTACTGCTTTTGAAATAGATCAAGATATGGTTGATTATTTACAAAAAAATAATATCTTAAATCAAAATCAAATTGTCCAAGGAGATTTTTTAATAGCTGATTTGAAAAAATATAAAAGCTATGTTGTTGTTGGGAATATTCCTTATTACATAACTAGTGATATTATTTTTAAACTATTAGATTATCGACACAATTTTAAAAAAGCTATTCTTATGGTTCAAAATGAAGTGGCTCAAAGATTAGTGGCTAAACCTAATCAAAATGATTACAGCAAACTTTCAATAACAGTTCAATATTGTGCTGATGTTAAAAAAGAATTATTTGTTAAGAAGACTTATTTTGATCCAGTTCCTAAAGTTGATTCTGCTATTGTTTCTATTACTTTTAAAAATGAAGCAAATGATAATTATGAAAATTTAAAAGACTTTTTCAAATTATGTTTTTTAGCTAGAAGAAAAAAATTATCATTTGCTTTAGCTACAAAATATAGTAATGATAAAATTAAAATTGCATATAATAAATTAAATTTAAAAGAATTAACTAGAATTCAAGAATTAGATTTAAAAACTATATTAGCTTTATACGATTGCTTAGAAAATTAG", "aa": "MFQENKLKAKKRYGQNFLKDQNVINKIIELIKPQGEKILEIGPGRGALTKILNQQAKEFTAFEIDQDMVDYLQKNNILNQNQIVQGDFLIADLKKYKSYVVVGNIPYYITSDIIFKLLDYRHNFKKAILMVQNEVAQRLVAKPNQNDYSKLSITVQYCADVKKELFVKKTYFDPVPKVDSAIVSITFKNEANDNYENLKDFFKLCFLARRKKLSFALATKYSNDKIKIAYNKLNLKELTRIQELDLKTILALYDCLEN", "aa_hexdigest": "8ce331cce275527d49d2396d62fefb7c", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001A8C2A5", "ncbi_nrp_id": "WP_013527117.1", "uniref100_id": "UniRef100_C4XF68", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2A5", "RefSeq:WP_013527117.1", "UniRef:UniRef100_C4XF68" ] }, "ips": { "uniref100_id": "UniRef100_C4XF68", "uniref90_id": "UniRef90_C4XF68" }, "psc": { "uniref90_id": "UniRef90_C4XF68", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_N9V0N0", "go_ids": [ "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_N9V0N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V0N0" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "EFPKPENHBP_1717", "locus": "GCA000186005_01710" }, { "type": "cds", "sequence": "contig_1", "start": 938563, "stop": 938766, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01026.27" ], "nt": "TTGCCAATTGATAGAATATTAACTGAAACTGATGCTCCTTTTCTTTCTCCAGCTCACAAAAGAGGTGAATTAAATTATCCAAATTATGTAATTCAAACAACTCACTTTATTGCAGGTATTAAAAAAATACCAATTGAAAAATTTACTGACCAGATTTTAAAAAATGCAAAGGATTTATTTAAGATAAATGTTTCAAGAAAATAA", "aa": "MPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK", "aa_hexdigest": "6f4369414dd83fa7ff7eea226a90ebd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01026.27", "name": "TatD_DNase", "length": 61, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.413857791908234e-16, "score": 56.94118118286133, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 7738.960099999998, "isoelectric_point": 9.62998104095459 }, "id": "EFPKPENHBP_1718", "locus": "GCA000186005_01711" }, { "type": "cds", "sequence": "contig_1", "start": 938860, "stop": 939336, "strand": "-", "frame": 1, "gene": "tatD", "product": "TatD DNase family protein", "db_xrefs": [ "EC:3.1.21.-", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_C4XF67", "UniRef:UniRef90_C4XF67" ], "nt": "ATGTCAATAAAATATATTGATGCTCATACTCATCCATTAAAAGAATACTATGATGACAATTATCATGTAATTGAAAGAGCTCATGCTAAAGGTTTAGTAGCTTTATTAATTACCGGATGTAATGAACAAGAAAATGAAGAAGTTATTAAAATTGCCAAAAACTTTTCATATACTTTTCCTGTTATTGGAATTCATCCAAATGAATGTCATGGAAAGGTTGACGGCGAAATTATTGAAAAGCAATTAGATGATTCTGTTGTTGCAATTGGCGAAATTGGTTTAGATTACTTTTATACACCAGAAAAGAAAGATATTCAAATTGAATCATTGCATGCTCAAATTCAAGTAGCTAAAAAACACAATTTACCAGTAGTAATTCACATGCGTGATGCATATGAAGATTTGTACGAAATTATTAAACAATATTATAAAGATGTTGTTTTTATGATTCATACATATAGTGGTAACTTATATTGA", "aa": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLY", "aa_hexdigest": "ddfea2fffa4234f3aed689ee36d5ef5a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "tatD" ], "truncated": "5-prime", "pseudogene": { "start": 938563, "stop": 939336, "observations": { "mutation": [ 938769, 938862 ], "stop": [ 938769, 938862 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF67", "query_cov": 1.0, "subject_cov": 0.6147859922178989, "identity": 1.0, "score": 323.0, "evalue": 1.42e-110, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLYWAKKFYELGCYFSFSGVATYKNGTETIEVLDWLPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK" }, "paralog": false, "identity": 0.9922178988326849, "score": 519.0, "evalue": 3.85e-190, "description": "Internal stop codon at 938769, 938862. Nonsense mutation around 938769, 938862.", "psc": { "uniref90_id": "UniRef90_C4XF67", "product": "TatD DNase family protein", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_C4XF67", "kegg_orthology_id": "K03424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF67", "EC:3.1.21.-", "UniRef:UniRef50_C4XF67", "KEGG:K03424" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF67" ], "product": "TatD DNase family protein" } }, "id": "EFPKPENHBP_1719", "locus": "GCA000186005_01712" }, { "type": "cds", "sequence": "contig_1", "start": 939440, "stop": 940384, "strand": "-", "frame": 3, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "GO:0003924", "GO:0005525", "GO:0006400", "SO:0001217", "UniRef:UniRef50_A5IXJ1", "UniRef:UniRef90_C4XF66" ], "nt": "ATGGCACTGCCAGGTGAATTTAGTCGACGTTCATTTTTGAATGGTAAAATGGACTTAATTAAAGCTGAAGCAATTAATGATTTAATACATGCCAAAACATTAAAACAAAGTGAATTAGCCATTAAGAAATTTGATGGCAAAACTTCAAACATGATTAAATCATTTATTGACGAATTAGCCTATTTAATAGGTCAAATGGAAATAAATATAGATTATCCAGAGTATGAAGATTTTGAAAATGTTTTGACAAAAGATTTAATTTCTAGATTGCAAAAATTGCAAGACAAATTGAGCTTAATTATCAAAGAAAGTGAAAACTCAAGATTAATTTTTGATGGCATTAAAGTTGCTATTCTTGGTAAGCCAAATGTTGGAAAAAGTTCAATATTAAATTGCTTAATAAATGAAGATAAAGCAATAGTTACAGATCAGGCTGGAACAACTCGTGATCTTGTTGAAGCTTCATATCAAATTGATGGTTTATTATTCAAATTAGTTGATACCGCTGGAATTAGAAAAACTAATCGAAAAATTGAAAAAATAGGTATTGAAAAATCTTTTGAACAAATTGAAAAATGTGATGTTGTAATTCATGTTAATGAAGCTAATACTTTAGAAAATGATTACGATAAAAAAGTTGAAAAATTAGCTAAACAATTTAACAAACCATTAATTAAAGTAATTAATAAAGTTGATTTATTAAAAAATAATAAAAAGAAAAAGAATGTTATTTATGTCAGTGCTAAAAATAAAAACATTGATGATTTAAAGGAAGCATTAGTTAATATTTTTTCAAAGAATGAAATTAACAATGAAGAATACGTTGTTAATGCAAGACAACTAGCATTGGTTAAAAAGGCTCATTCAAGTATTTCTGATGCAATTGAATCAATAAATAATGGCATTGATGTTGATGTAGTAATTATTGATGTAAGACAAGCTTGA", "aa": "MALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQA", "aa_hexdigest": "d6cd6218eb82f148bc43dd1d7491eff9", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmE" ], "truncated": "both", "pseudogene": { "start": 939356, "stop": 940684, "observations": { "mutation": [ 939442, 940498 ], "stop": [ 939442, 940498 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF66", "query_cov": 1.0, "subject_cov": 0.6977777777777778, "identity": 1.0, "score": 587.0, "evalue": 3.83e-209, "gene_start": 1, "gene_end": 314, "reference_start": 109, "reference_end": 422, "reference_sequence": "MYIMINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIAWFIGNKNFTGENTVEINCHGGVLVTNRILELLLANGARMALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQAWADLVDISGRADNELLLDEMFKNFCLGK" }, "paralog": false, "identity": 0.995475113122172, "score": 842.0, "evalue": 2.7e-312, "description": "Internal stop codon at 939442, 940498. Nonsense mutation around 939442, 940498.", "psc": { "uniref90_id": "UniRef90_C4XF66", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_A5IXJ1", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF66", "UniRef:UniRef50_A5IXJ1", "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXJ1" ], "product": "tRNA modification GTPase MnmE" } }, "id": "EFPKPENHBP_1720", "locus": "GCA000186005_01713" }, { "type": "cds", "sequence": "contig_1", "start": 940496, "stop": 940699, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATGACACAATTGCAGCAATATCTTCAGGTGGTAAGATTAATCAAGCCATATCAATAATCAGAGTTAGTGGTCAAGATAGTATTGAAATAGTTTCTAAAATTTTTAAAGGAAAAATAGGAACAAATCAAACAATAACTTATGGCCATATTTTTGATAATAAAACAAAAGAAATTATTGATGAAGTTTTAATTGCTTGA", "aa": "MINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIA", "aa_hexdigest": "16c3ea807b913ca42a1c14ac7cfdd624", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.255399999998, "isoelectric_point": 6.533664894104004 }, "id": "EFPKPENHBP_1721", "locus": "GCA000186005_01714" }, { "type": "cds", "sequence": "contig_1", "start": 940770, "stop": 941456, "strand": "+", "frame": 3, "gene": null, "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354931.1", "SO:0001217", "UniParc:UPI0001E32FDC", "UniRef:UniRef100_C4XF65", "UniRef:UniRef50_A0A3P8MDF4", "UniRef:UniRef90_C4XF65" ], "nt": "ATGAATAATCAAAATTTTAAGAATTTATTAACTAAATATATTGAATTCTTTTATAGGTATAAAAGCATTTCATTATTAAAAAAGGCAAATAATGAATCACAATTTGATGTTAAATTAAGATTAGTTTTAACACAAAAATTTTTACTGCATTCACTAGGTTTGCAATACAACAAAAAGTTATTTAGAAAATATAAGAACAATAATTTTGAACTTTATAGAGATATAAAAAATGATCAACTAAATTATGATGAGTTAATTAAAGACATAAGCAAAAACAAAAAAGATTTAATTGACAAAAAATTAAACGCTCTTGAGATTTTTGTCAATATTATAAAAAATATTAATCAAGAAAAACTATTTACTTTTGCATTTGAATTTTTTAAATTAAAACCAAAATTTGATATTGATGGAGCTTATAACCATTCACAATTGATTTTAGTTACAAAAAGCAATAAAGAAAATCAAAGATGTATTATTTATGGAATTTTGGGAGATTTATATACCACCAATTCAAAAGATAATAATCATTTTTTAAACGAAAATAAAAATCTTATGAACACAGTTTTTATACCTATATCAATAAAAATCAAAAACAATGAAAATCTTTTAAATTCTCAAAATATACAAAAAATTGATATTGCATTTTTAAACAAAAGAAGAATACAAGAAAAAGAAATAAGAGTATAA", "aa": "MNNQNFKNLLTKYIEFFYRYKSISLLKKANNESQFDVKLRLVLTQKFLLHSLGLQYNKKLFRKYKNNNFELYRDIKNDQLNYDELIKDISKNKKDLIDKKLNALEIFVNIIKNINQEKLFTFAFEFFKLKPKFDIDGAYNHSQLILVTKSNKENQRCIIYGILGDLYTTNSKDNNHFLNENKNLMNTVFIPISIKIKNNENLLNSQNIQKIDIAFLNKRRIQEKEIRV", "aa_hexdigest": "7616135b0e1370eea83caffd82d75333", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDC", "ncbi_nrp_id": "WP_013354931.1", "uniref100_id": "UniRef100_C4XF65", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDC", "RefSeq:WP_013354931.1", "UniRef:UniRef100_C4XF65" ] }, "ips": { "uniref100_id": "UniRef100_C4XF65", "uniref90_id": "UniRef90_C4XF65" }, "psc": { "uniref90_id": "UniRef90_C4XF65", "uniref50_id": "UniRef50_A0A3P8MDF4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDF4" ], "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1722", "locus": "GCA000186005_01715" }, { "type": "cds", "sequence": "contig_1", "start": 941494, "stop": 941817, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGCCTGATCCAAATGCTTTAACAGAAGTTAAATCAACTTCAAGTGCTTCTGGTGAAGAAACTCCAACAGATTCGAAATTAAATAACCTAAGTCTAAAAGCAAAAGCTATTGTTGAAGCTGTTGGTGGTTACGAAAACATTACAACTTTTGCAAACTGTGCAACAAGACTTCGTTATGAAGTTAAAGACTCAGATATTGTTGATGAAGCAAAATTAAAACTTGCGGGATGCAGTGGTTTGATTAGATTAGGAAAGAATTCAATTCAAATTATTGTAGGTGTTGAAGCTGAAAACATTAACAATGAAATTGAATCAAATAAATAA", "aa": "MPDPNALTEVKSTSSASGEETPTDSKLNNLSLKAKAIVEAVGGYENITTFANCATRLRYEVKDSDIVDEAKLKLAGCSGLIRLGKNSIQIIVGVEAENINNEIESNK", "aa_hexdigest": "5af06d39b2de6278ce5dade57c7f8897", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 34, "aa_cov": 0.3177570093457944, "hmm_cov": 1.0, "evalue": 1.8657058093829748e-16, "score": 55.58298110961914, "start": 35, "stop": 68 } ], "seq_stats": { "molecular_weight": 11426.709099999993, "isoelectric_point": 4.8286657333374015 }, "id": "EFPKPENHBP_1723", "locus": "GCA000186005_01716" }, { "type": "cds", "sequence": "contig_1", "start": 941920, "stop": 942264, "strand": "-", "frame": 1, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "nt": "ATGTTTGGTCTTCCTGCTCTAGCATTTGCTTTCTACATGACTGCAGATGATAAAGTGCAAAAGAGAAGAGTAATGGCTATCTTTGGAGCAGCCGCAGTAGTATCAATTCTTTCAGGTATTACTGAACCTATCGAATTTGCATTTATGTTTGTTAGTCCATTATTATATGGTTTCCACTGCATTCTAACTGGTATATTTGCATTTATAACTGGTGCATTTGGAATTCAAATTGGATTTGGGTTCTCAGCTGGTTTAATGGATTACTTATTATCAATTCCTAAATCATTAGCAATTATTAAAGCTAACAAAACAGGTATTGATGCTGTATTAGCTAACCCTGGTTGA", "aa": "MFGLPALAFAFYMTADDKVQKRRVMAIFGAAAVVSILSGITEPIEFAFMFVSPLLYGFHCILTGIFAFITGAFGIQIGFGFSAGLMDYLLSIPKSLAIIKANKTGIDAVLANPG", "aa_hexdigest": "d33d0bc54d09b9fc48e069f20f333793", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0016429E17", "query_cov": 0.8070175438596491, "subject_cov": 0.9019607843137255, "identity": 0.5329999999999999, "score": 91.3, "evalue": 9.15e-22, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0035A088D1" }, "pscc": { "uniref50_id": "UniRef50_UPI0035A088D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "EFPKPENHBP_1724", "locus": "GCA000186005_01717" }, { "type": "cds", "sequence": "contig_1", "start": 942302, "stop": 942526, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCCTTTTGCTTTACCACTTAAGAAAATAAAGATATCACCAAATACAGGTGTACCATCAGGAGTTACATGTTCTCCTAATTGGAATCAGAATAAGTTGTTTGGAATGTGGTGTAATCCAAATGGAATTAATAGACGATTAATAACGCCATATACACCCATAATAGCAGCTGAAGCTCAACGACCTTTAGCTGCACCTGATAACACTTTTGAAAATGCATAA", "aa": "MFPFALPLKKIKISPNTGVPSGVTCSPNWNQNKLFGMWCNPNGINRRLITPYTPIIAAEAQRPLAAPDNTFENA", "aa_hexdigest": "08b603faac097bd854914613f42c5a16", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8155.374299999995, "isoelectric_point": 9.589817237854003 }, "id": "EFPKPENHBP_1725", "locus": "GCA000186005_01718" }, { "type": "cds", "sequence": "contig_1", "start": 942571, "stop": 943248, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTACAAAAACACAATCATCAAGACCTTCATTTGGAAAAAGACTATTAGAAAAACTTGGACGCATTGGTCGGGTATTGATGTTCCCTATTGCAGTTCTTCCTATTGCTGCTATTCTTCTTAGAGTTGGAGCTGATTTACCAACAGTTAAAGCTGGACCTGATATTTCACAAGTCGAAGCTAACTTTGTTAACTTTGTTCAACAAATGATAACAGCAGGCGGATCAGTTGTATTTGACAACTTGTACATTTTATTTGCCGTCGGAATAGGATTTGGTTTTACTAAAGATAACCGTGGTGAAGCTGCTTTTGCTGCTTTAATTAGTATTCTATTACTTTCACTTTTATTAAAAAGTGGTGGATATGTAGATAAAATCTACAGCACATTTAAACTCCCTGGAGCCGAATTTAATGAAGATGGTAAAAAACTTGAAGCTTTTATAGCTGAAGGTAAACCTGCTTCAGAATTTGTTATAGCTAATGGTTTTGCAGGTGTCTTTGGTGGCAAATACAATGCAATAATGTCTGGCAATGTACTTAATGGTATTATTGTTGGATTTATTGTTGCAGCTATTTATAACCATACAAATGATGTTCAATTACCTAAAGTTCTTGGATTCTTTTCGGGTAGAAGATTAGTTCCAGCTCTTGCAATTCTTTCAACTCTTATTTGA", "aa": "MTGTKTQSSRPSFGKRLLEKLGRIGRVLMFPIAVLPIAAILLRVGADLPTVKAGPDISQVEANFVNFVQQMITAGGSVVFDNLYILFAVGIGFGFTKDNRGEAAFAALISILLLSLLLKSGGYVDKIYSTFKLPGAEFNEDGKKLEAFIAEGKPASEFVIANGFAGVFGGKYNAIMSGNVLNGIIVGFIVAAIYNHTNDVQLPKVLGFFSGRRLVPALAILSTLI", "aa_hexdigest": "eab4b7a4d83c5d0a21045785c86786e9", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23917.7978, "isoelectric_point": 9.58169422149658 }, "id": "EFPKPENHBP_1726", "locus": "GCA000186005_01719" }, { "type": "cds", "sequence": "contig_1", "start": 943248, "stop": 943451, "strand": "-", "frame": 2, "gene": null, "product": "DUF4044 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527119.1", "SO:0001217", "UniParc:UPI0001F598D7", "UniRef:UniRef100_C4XF63", "UniRef:UniRef50_A0A3P8MEH3", "UniRef:UniRef90_C4XF63" ], "nt": "ATGGTTTTAATTATTATCTTAGCTTCTATTAAGAACTTCGATGCTGCAGAATCTTTAAAAACAACAAATGCTAAATACGTAGCCATTGTTGCCTTATTTTTCTTTATATCTTTATGTGCTGCAATTTTTGGAACAGTTATTTTATCAATCTTAGATAAAAAAGCTGTTACAAAAAAAGCACAACAAAATCAAGGAGGTAAATAA", "aa": "MVLIIILASIKNFDAAESLKTTNAKYVAIVALFFFISLCAAIFGTVILSILDKKAVTKKAQQNQGGK", "aa_hexdigest": "03fee40d101b04b203a052afb30f3c4b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F598D7", "ncbi_nrp_id": "WP_013527119.1", "uniref100_id": "UniRef100_C4XF63", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598D7", "RefSeq:WP_013527119.1", "UniRef:UniRef100_C4XF63" ] }, "ips": { "uniref100_id": "UniRef100_C4XF63", "uniref90_id": "UniRef90_C4XF63" }, "psc": { "uniref90_id": "UniRef90_C4XF63", "uniref50_id": "UniRef50_A0A3P8MEH3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEH3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEH3" ], "product": "DUF4044 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1727", "locus": "GCA000186005_01720" }, { "type": "cds", "sequence": "contig_1", "start": 943765, "stop": 944466, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013354934.1", "SO:0001217", "UniParc:UPI0001E32FDF", "UniRef:UniRef100_C4XF62", "UniRef:UniRef50_C4XF62", "UniRef:UniRef90_C4XF62" ], "nt": "ATGAAGAAAAACTTATTAAAGTTAACAGGAATTATTGCTCCAGTTATTACAGCTATACCATTTGTTGCTTCATCATGTGGTAGTAAAGATGAAGCAACTTTAGCTGATCTTTTAAAGGAAAAATTGACAAAGGATGCAGATGCTTCTAAATATTCTGCACTAATTACTGACAAGGAAAAATTAAAACAATTCAAGGAATTAGTAGAAGAATTTAAAAAAGAAGGAGTAGATTTAACTGCTAAAGGTAATCCTTCTGATGTAGATAATGCAATTAAAACTATTAAAGAAAAAGCGCCATTAGTTTTAGCTAGAACAATTAAAGCAATGGTTGAAAACGGAAGTGCTTTTATAATTAAAGAAGATGGGATTGCTAGTGCTAAACAAAAACTAGAAAATGGTGATATATTTGCTCAAGTTTATCCTGCACAACATAAAATCGGAAGATCTGCTTATACAATTATTGAATTTACTGAAAAATTTAAGGCATTTTATTTAACTTCAAAAGTATTCGAAGTTGCTAGAATTTCAACATTTTATGAAAATAAATATGGTGGTAAAACTCAACCTCATGCAAACGGAGTAAACTTAGAATTTGTTAAAGGCAAGTTAAATATTAAATTTGTGATTTGTGATTTGCTAAAAGGCGACCAAACTGGTTCAGTTGTTTTAGTAAATAACTTATTATTTGCAGTTGAAAAATAA", "aa": "MKKNLLKLTGIIAPVITAIPFVASSCGSKDEATLADLLKEKLTKDADASKYSALITDKEKLKQFKELVEEFKKEGVDLTAKGNPSDVDNAIKTIKEKAPLVLARTIKAMVENGSAFIIKEDGIASAKQKLENGDIFAQVYPAQHKIGRSAYTIIEFTEKFKAFYLTSKVFEVARISTFYENKYGGKTQPHANGVNLEFVKGKLNIKFVICDLLKGDQTGSVVLVNNLLFAVEK", "aa_hexdigest": "4ad2567d9b653639e138017a546a76b7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDF", "ncbi_nrp_id": "WP_013354934.1", "uniref100_id": "UniRef100_C4XF62", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDF", "RefSeq:WP_013354934.1", "UniRef:UniRef100_C4XF62" ] }, "ips": { "uniref100_id": "UniRef100_C4XF62", "uniref90_id": "UniRef90_C4XF62" }, "psc": { "uniref90_id": "UniRef90_C4XF62", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XF62" }, "pscc": { "uniref50_id": "UniRef50_C4XF62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF62" ], "product": "Lipoprotein" }, "genes": [], "id": "EFPKPENHBP_1728", "locus": "GCA000186005_01721" }, { "type": "cds", "sequence": "contig_1", "start": 944635, "stop": 945051, "strand": "-", "frame": 1, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "ATGACAACAGTTCACGCTTACACAGGTGACCAAATGTTACAAGATGGTCCACACCGTAAAGGTGACTTAAGACGTGCTCGTGCTGCTGCTCAAAACATTGTTCCTTCAACAACAGGTGCTGCAAAAGCTATTGGTTTAGTTGTTCCCGAAGCTGATGGATTATTAGATGGTTCAGCATTGCGTGTTCCTACAATTACAGGTTCATTCGTTGACTTAACAGTTCAATTGAAAAAACAACCTACAGTTGAAGAAATTAATGCTGCATTCAAAAAAGCAGAAAACGAAACAATGAAATATGTAGTTGACCCTATTGTTTCTTCAGACGTTATTAACTCACACTATGGTTCATTATTCGATTCAACTTTAACAAAAGTTAAAGAAGGAAATGGTCAAAGACTTTACAAAATCTTTGCATGA", "aa": "MTTVHAYTGDQMLQDGPHRKGDLRRARAAAQNIVPSTTGAAKAIGLVVPEADGLLDGSALRVPTITGSFVDLTVQLKKQPTVEEINAAFKKAENETMKYVVDPIVSSDVINSHYGSLFDSTLTKVKEGNGQRLYKIFA", "aa_hexdigest": "6b35d1e376449254ce947b31e6efdcc8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 1.0, "subject_cov": 0.8679245283018868, "identity": 0.703, "score": 192.0, "evalue": 1.95e-60, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "EFPKPENHBP_1729", "locus": "GCA000186005_01722" }, { "type": "cds", "sequence": "contig_1", "start": 945316, "stop": 945573, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAGTTGCAATCAACGGTTTCGGTCGTATTGGACGTTTAACACTTCGTCACTTACTTGAAACCCAAAATAAAGAAGTTCAAGTTGTTGCAGTTAACGACTTAACAGATCCAAAAATGTTAGCACATTTACTTAAATACGATACAGCTTTTGGCCCATTGGAAGCTAAGGTTGAAGTTAAAGAAAATGCTATTAAAGTTAATGGAAAAGAAATTAAAGTATTCTGCGAAAAAGATCCAGCTAACTTACCATGA", "aa": "MKKVAINGFGRIGRLTLRHLLETQNKEVQVVAVNDLTDPKMLAHLLKYDTAFGPLEAKVEVKENAIKVNGKEIKVFCEKDPANLP", "aa_hexdigest": "c073492bc640cadb5a1b7ec5609d0565", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9764705882352941, "subject_cov": 0.8469387755102041, "identity": 0.6509999999999999, "score": 104.0, "evalue": 1.29e-27, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 83, "aa_cov": 0.9764705882352941, "hmm_cov": 0.9759036144578314, "evalue": 7.292521442049344e-31, "score": 102.83222198486328, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9498.060999999992, "isoelectric_point": 9.131253623962404 }, "id": "EFPKPENHBP_1730", "locus": "GCA000186005_01723" }, { "type": "cds", "sequence": "contig_1", "start": 946060, "stop": 946260, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACCAATTTTAGTAAGATTATTAAATGGAATTTATGCTGTTAGTTGTGCATTACAAATAGTATTGCCTACAAAAATAAAAGAAGTTGCAAGTGTTTTAAAAGTTAAAAAATTTGACTTTGAAAAAATTAATGATTTCAATAAATTTGATAAAGTTATTTGTGATGAGAAATATTTGTTATTTAATAGATTAAAATAA", "aa": "MEPILVRLLNGIYAVSCALQIVLPTKIKEVASVLKVKKFDFEKINDFNKFDKVICDEKYLLFNRLK", "aa_hexdigest": "609aac8a8f3aae22747e0ef7c4f0caa4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7704.206999999998, "isoelectric_point": 9.23040599822998 }, "id": "EFPKPENHBP_1731", "locus": "GCA000186005_01724" }, { "type": "cds", "sequence": "contig_1", "start": 946285, "stop": 946680, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAATCTTTAAATAATGTTATAGCCCCAGATTATTTATTTGACAAGTATCATCATGAGATGATTAAATACTATTTTGCAACTCAAATAATATTTGGAGAAGATGGTAATTTTAGTGAAGAAAAATTAAAAGAAGTTGTTAATGCAGATTTAATAAATAATTATGGTAATTTAGTTTCAAGAACACTAAAAATGATTCACAATTCTTTCCCAAAAGGTCTGCTTTATAGTGTTAATAGTAAAAAAATACATAAAGACATTGAAAAAGAAATTAAGAATTTCAGTGCTCAATTTCAAAAACATATGGACGCTTTTGAAATTGATAAAGGATTTAAAAAAGCAATTGAATTATCAGCTAAATTAAATAAATATATTGATGAAACAACACCATGA", "aa": "MSKSLNNVIAPDYLFDKYHHEMIKYYFATQIIFGEDGNFSEEKLKEVVNADLINNYGNLVSRTLKMIHNSFPKGLLYSVNSKKIHKDIEKEIKNFSAQFQKHMDAFEIDKGFKKAIELSAKLNKYIDETTP", "aa_hexdigest": "4486b9ec4ed3157aafe97fb5be130175", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15257.295799999984, "isoelectric_point": 7.874249076843262 }, "id": "EFPKPENHBP_1732", "locus": "GCA000186005_01725" }, { "type": "cds", "sequence": "contig_1", "start": 947509, "stop": 947604, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAAAGACTTTTTATATAACTACTCCTATTTATTATGCTTCAGGTCCGCTTCATATCGGACATCTTTATTGTACAATTATGGCTTGA", "aa": "MKKQKTFYITTPIYYASGPLHIGHLYCTIMA", "aa_hexdigest": "47b9a5c2cc8114a57fd64ec4c252bdb6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3591.2688000000007, "isoelectric_point": 9.361470222473145 }, "id": "EFPKPENHBP_1733", "locus": "GCA000186005_01726" }, { "type": "cds", "sequence": "contig_1", "start": 947724, "stop": 948392, "strand": "-", "frame": 2, "gene": null, "product": "Methyltransferase small domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_C4XF58" ], "nt": "ATGAATAAAAAACAAAAATTAGTTAAAAATTCTTTAGGTTTTGATTCTAATTTATATGTTGTGCAAGACAAAGATATGTTCAACTATTCAGTTGACACTATTATGCTTGGCAACTTTGTTTTTTTAAATAATAAAATTAAAAACCTATTAGAAATTGGAGCAAACAATGGAGCTCTCAGTATTTTTATAGCTGAAAGAAATCCAAATTTAAAAATAGATGCAGTTGAAATCCAAACTAAAGCTTGTAAGTTAGCTCAAGACAATATTGAACTTAACAACAAGCAAAATCAAATCAAAATAATTAATCAAGATTTCAATGAATTTTATTTAGAAAAAATTAAAAATGCAGCTAGTAAATATGACTCAATAGTTTGCAATCCTCCTTTTTATACAATGCAAACTAATAAAGTTAGCAAAAATATTAGTGAAGAATTACTAATTGCAACACATGAGTATAAAATTAATTTAGAACAAATTATTTTAGGTTCTTCAAAATTAATTGAGCAAAAAGGCTACTTAACTTTAGTGATTCCAGTTGAACGCTTAGTTGATTGCTTTTGCTTAATGAGAAAATATAATTTTGAGCCTAAAAGAGTTCAATTCATTATTCCAAGAATGCAAGATAAACCTAAATTAGTTTTAGTTGAAGGCAGATATCAAGCTGGTTGA", "aa": "MNKKQKLVKNSLGFDSNLYVVQDKDMFNYSVDTIMLGNFVFLNNKIKNLLEIGANNGALSIFIAERNPNLKIDAVEIQTKACKLAQDNIELNNKQNQIKIINQDFNEFYLEKIKNAASKYDSIVCNPPFYTMQTNKVSKNISEELLIATHEYKINLEQIILGSSKLIEQKGYLTLVIPVERLVDCFCLMRKYNFEPKRVQFIIPRMQDKPKLVLVEGRYQAG", "aa_hexdigest": "bc2ae9df8dc10fd8e62d00e2fbd2b347", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XF58", "query_cov": 1.0, "subject_cov": 0.844106463878327, "identity": 1.0, "score": 432.0, "evalue": 1.84e-152, "valid": true, "product": "Methyltransferase small domain-containing protein", "uniref50_id": "UniRef50_A0A448ZXG4" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [], "id": "EFPKPENHBP_1734", "locus": "GCA000186005_01727" }, { "type": "cds", "sequence": "contig_1", "start": 948395, "stop": 950578, "strand": "-", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "RefSeq:WP_013527121.1", "SO:0001217", "UniParc:UPI0001F598D9", "UniRef:UniRef100_A0AB32XCX4", "UniRef:UniRef50_A5IXZ7", "UniRef:UniRef90_C4XF57" ], "nt": "ATGAATCCGCAAGAAATATTAAATTTTTTAAAAAAACATGATAATTCAACTTTCATTGATATTGCAAAAGTCTTAAGAATAAGTTATAAAGATAACAAAAAATTAACCGAATTACTAAATAAATTAATTGATGAAAGAAAATTAATTAAAAATGGCAAACTCTCAACTTATTCATTAATAGAAAAAATTAAAATCGTCCAAGGAAAAATTCGATTTGCTTCAGAAGGCAAGTATGCTTTTGTAGAAGAAGAAAATGATAATCCTGAAGCTGAAAAAATAAGTTATTTTGTACCTGGTATTCATCTTAATAGAGCATTAGATGGAGACTTTGTTGAAATAACAGTTTTAAAATACTTTCATTCTTTAGATGATAAAACTTTTGCAATTGTTGAAAAAGTTTTAAGCAGAAACAGTGATAAATTCATTGGTATTTTAGGTATGCATGAAGGTTTTCTTTCATTTAAACCACTTAAAAATGACTACAAAAAAATTAATTTTCATATTAAAGAATTGGTTAAAGAAGCTAGACTTCATGATGTTGTTTTAGGTCAAATGGTTTCATTTAAAAATAATATTATTGAGCTTAACATTATCAAAAAAATAGCTAACAGTAATGATCCAATGGCTTATGTTAAAGCCTTAACAGTTATTAGAAATAAACCTGAAGATTTCCCACAAGAAGTTAAAGATTACTTAGTTAAAATTCCAAATAGTATTGCAGATGAAAAACTTAATAATAGACTTGACTTGAGAAAAGAATTAATTGTTACTATTGATGGTAATGACACAAAAGACTTTGATGATGCAATTAATGTTAAAAAGAATGCTGATGGAACTTATGAATTAGGCGTTCATATTGCTGATGTTAGTTATTATGTCAAAGAAGACAGCTCTCTTGATGTTGAAGCATTAAAAAGAGGAACCTCAAGATATTTAGTTAGTGGCGTTATTCCAATGTTGCCTCATAAATTATCAAATGGTATTTGCTCACTTAATCCTAATGAAGATCGCTTTACTCTTAGTGCAATAATGACAATTGATAAAAATGGTGATACTATTGATGCTAAATTTTATCAGTCAATAATTCAATCAAAATATCGTCTCACATATGATAGGGTTAATGAACTTTATGATAAAAATCAAAAATTTGATGACCCGAAATTAAATCGAATGCTCTATGAAGCAGCTGAATTGGCAAAAATAATTAGAAATTATAAAGTCAAAGAAGGCTACATTGATTTTGAAATTGAAGAACCCGAAATTGTCTTAGATGATAAAGGTAAAACAATTGATGTTGTAGTTAAGCCAACTGGTTTTAGTGAAAATCTTATTGAAGATTTCATGGTTAGAACTAATGAAGAAGTAGCTAAATTTTTAGCTAAAAGAAAAATACCTTTTATTTATCGTGTTCATGAAAGCCCTGATAGTGACAAAATTGATTCATTTTTGAGTGTACTTAATGTTTTAGGTATTAAAGTAAACATTGATCGAAATGATATTAATCCAAAAAGTTTTCAAAAAATAATCAACAAAATTAAAGAAACAAGAAATGATGACTTTTTAAAAACAGTTTTCTTAAGAACAATGCAAAAAGCTAAATATTCTACAAATAATATTGGCCACTTTGGTTTAGCAAGTGAACACTATTGTCACTTTACTAGTCCAATTCGACGTTATCCAGATTTAGTTGTTCATAGGATTTTAAGAGAAATTGTTTTTAACAATGATAAAAGTAAAATTACTCACTACAAAAACATTTTAAGTAATGTTGCAGAATTAAATTCAAATAGTGAACAAGAAGCATTACAACTTGAACGTGATACAAATGATTTAAAATATGCTGAGTTTTTCAAAAACAAAATTGGTCAAACAATGAAAGCTCAAATTATTAGTATTTTACGCTTTGGTATGTTTGTCCAATTTGACTGCAAAATAGAGGCTTTAGTTCATATTTCAACATTATGTGATGGTGATTATGAAGTTAATGAAAACTTTACTGAATTAAGAAGTAAACAACACACTTTTAAACTTGGAGACCAAGTAGAAGTGGTTATTGCTGGAGCTGATGAAACTAACGGTAAAATTGATGCAGTGCTTGCAAAATGCTATGGTTCATACTTAGAAGAAATCAAGAAAAAACTTAATTCAAACTCAAATCGCAAACATAAAAAATCAGGTAAATAA", "aa": "MNPQEILNFLKKHDNSTFIDIAKVLRISYKDNKKLTELLNKLIDERKLIKNGKLSTYSLIEKIKIVQGKIRFASEGKYAFVEEENDNPEAEKISYFVPGIHLNRALDGDFVEITVLKYFHSLDDKTFAIVEKVLSRNSDKFIGILGMHEGFLSFKPLKNDYKKINFHIKELVKEARLHDVVLGQMVSFKNNIIELNIIKKIANSNDPMAYVKALTVIRNKPEDFPQEVKDYLVKIPNSIADEKLNNRLDLRKELIVTIDGNDTKDFDDAINVKKNADGTYELGVHIADVSYYVKEDSSLDVEALKRGTSRYLVSGVIPMLPHKLSNGICSLNPNEDRFTLSAIMTIDKNGDTIDAKFYQSIIQSKYRLTYDRVNELYDKNQKFDDPKLNRMLYEAAELAKIIRNYKVKEGYIDFEIEEPEIVLDDKGKTIDVVVKPTGFSENLIEDFMVRTNEEVAKFLAKRKIPFIYRVHESPDSDKIDSFLSVLNVLGIKVNIDRNDINPKSFQKIINKIKETRNDDFLKTVFLRTMQKAKYSTNNIGHFGLASEHYCHFTSPIRRYPDLVVHRILREIVFNNDKSKITHYKNILSNVAELNSNSEQEALQLERDTNDLKYAEFFKNKIGQTMKAQIISILRFGMFVQFDCKIEALVHISTLCDGDYEVNENFTELRSKQHTFKLGDQVEVVIAGADETNGKIDAVLAKCYGSYLEEIKKKLNSNSNRKHKKSGK", "aa_hexdigest": "f010ae905fa2d98e179765669ea1abfa", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001F598D9", "ncbi_nrp_id": "WP_013527121.1", "uniref100_id": "UniRef100_A0AB32XCX4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598D9", "RefSeq:WP_013527121.1", "UniRef:UniRef100_A0AB32XCX4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCX4", "uniref90_id": "UniRef90_C4XF57" }, "psc": { "uniref90_id": "UniRef90_C4XF57", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_A5IXZ7", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXZ7" ], "product": "Ribonuclease R" }, "genes": [ "rnr" ], "id": "EFPKPENHBP_1735", "locus": "GCA000186005_01728" }, { "type": "cds", "sequence": "contig_1", "start": 950582, "stop": 950818, "strand": "-", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "RefSeq:WP_013354940.1", "SO:0001217", "UniParc:UPI0001E32FE4", "UniRef:UniRef100_C4XF56", "UniRef:UniRef50_A0A4Y6I792", "UniRef:UniRef90_C4XF56" ], "nt": "ATGGCAGTTATATTTGTATCAATAATTATCGCTATTTTATCGATCGCAATTATTGTGATTTCATTGCTTATGTCACCAGACAGTAATGGTTTTTCTGGTGCACTTGTTGGTAGTGGTGATTTGGAACTTTTTAAATATTCAAAAGAAAGAGGACTTAAAAAGGTTTTAAAATACTCAATGTTATTTGGTGGAATAGCATTGCTAATTTTAGCTATTATTTTAAGGATTATTGTATAA", "aa": "MAVIFVSIIIAILSIAIIVISLLMSPDSNGFSGALVGSGDLELFKYSKERGLKKVLKYSMLFGGIALLILAIILRIIV", "aa_hexdigest": "9aa2fc4addf0534ccd25b6c256a57b1c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FE4", "ncbi_nrp_id": "WP_013354940.1", "uniref100_id": "UniRef100_C4XF56", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FE4", "RefSeq:WP_013354940.1", "UniRef:UniRef100_C4XF56" ] }, "ips": { "uniref100_id": "UniRef100_C4XF56", "uniref90_id": "UniRef90_C4XF56" }, "psc": { "uniref90_id": "UniRef90_C4XF56", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_A0A4Y6I792", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I792", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I792" ], "product": "Protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "EFPKPENHBP_1736", "locus": "GCA000186005_01729" }, { "type": "cds", "sequence": "contig_1", "start": 951032, "stop": 951964, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55", "UniRef:UniRef90_C4XF55" ], "nt": "ATGAAAAACAAAATAACCAAACACAATATGTGTGAAATTTATGAGTCATTTCGTTTTATTCAACCTGAATATAAAAGTCGTGTAAATTCATTAAAAAATAAAAATGAAAATGACGAATTTTTAATCAAAGCACAAATGGAAAGTGCTATTTCATTGAATGAATATATTGATTCAAAATTAGAAAGAAAAAAGATCAGAATTGCTAATCGTAAGATTTCAAAAATCTCAAGTATCTTTATTCCACTAATTGTTTTCTTCTTAGTAACTGTTTTAATTGCAGTGTCTTATATTGCTTATGTAGCAGCTAACTATATGGTTAAAGATGCTGAAGTTTATACACTATTTGGTATAGCTATATTATCACTTATTTCAGCAGCAATATGCTTTGTAGTTTACTATAGAAAATACACGCGATATAACAATGATAAGGCTAAATATTTTGAAGATAAATTAAAAGAAGAAATCAAAATTGGAGATTTAAAATCTATAGTTTTAAAAAACAAAAATACATTAAATTATCAAGAAGATGTTGCTTCATTAGTTGTTGCTAACGATCTAAAATATCCTGATATTCCTGCAGGCTACAAAGTTGAAGCTTCAAAATACGCTAAACGTTTTACACTATATGGAATGAATGTTTTAGCACAAGATGCTCAACTAGTAAAAATTGACGAAAAGGCTAATGAAACAGCTGAATCAGACAAAAGTATTATTAGTAATAAAGCTAAAAAAATTAAGATTTTTGAATTCTCATTTATGAATAGTCAATTTGACAAAATTTATTTACCATTTAACTTATTTAACAAATTTGAAGTTTTCAAAGCTAAACTTGAACCAATCAAAAGTAACGTTTCTGATTTAGACAAAATCTTCAATATTCATGCAATCAAAAACCCGCTTAACAGTAAACTTTTCGATGCTCTTAAATTATGA", "aa": "MKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYVAANYMVKDAEVYTLFGIAILSLISAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKL", "aa_hexdigest": "39f1f78ea27be4cedf8b794798c5ee14", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 951017, "stop": 952267, "observations": { "mutation": [ 951962, 952103, 952166 ], "stop": [ 951962, 952103, 952166 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF55", "query_cov": 1.0, "subject_cov": 0.7110091743119266, "identity": 1.0, "score": 577.0, "evalue": 1.26e-205, "gene_start": 1, "gene_end": 310, "reference_start": 6, "reference_end": 315, "reference_sequence": "MYGVSMKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYVAANYMVKDAEVYTLFGIAILSLISAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKLWKNNIHFAHTFIPQESGVNYFNFKLTAIFTLKEECEDEFNIQTNAKYWNNPFKVELPETIDDSTKLPMWFIINNFYKMVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI" }, "paralog": false, "identity": 0.9879807692307693, "score": 803.0, "evalue": 3.28e-297, "description": "Internal stop codon at 951962, 952103, 952166. Nonsense mutation around 951962, 952103, 952166.", "psc": { "uniref90_id": "UniRef90_C4XF55", "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF55", "UniRef:UniRef50_C4XF55" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35912.49940000003, "isoelectric_point": 9.37848987579346 }, "id": "EFPKPENHBP_1737", "locus": "GCA000186005_01730" }, { "type": "cds", "sequence": "contig_1", "start": 952193, "stop": 952327, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGACTATATTGCACTTTCAAAAGCAACAGTTGAAAAATATGTTATGCCAATGATATTCTACTTATTTAATGAAATTAATGACACCAAAGTATTTAATGAAAAAACTCTTTCAGATTTATACGAAATTTAA", "aa": "MVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI", "aa_hexdigest": "6448879fe5c36b8893f9243f0f1e2b57", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5241.0548, "isoelectric_point": 4.440682792663575 }, "id": "EFPKPENHBP_1738", "locus": "GCA000186005_01731" }, { "type": "cds", "sequence": "contig_1", "start": 952417, "stop": 953025, "strand": "+", "frame": 1, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "EC:2.7.4.8", "GO:0004385", "GO:0006163", "SO:0001217", "UniRef:UniRef50_Q4A6S7", "UniRef:UniRef90_C4XF54" ], "nt": "ATGAATATTATGAACAATTTAATTAGCAAACGTAAGCCAATAATAATATTTACAGGACCTAGCGGAGTTGGCAAAGGAACAATTGAAAAGTACCTTTTTGATTTTAAAAATTTAAAATTACATTTATCATGTTCAGCAACAACTAGAGCTCCTCGTGAAGGTGAAATTAATGGCATTCATTACTTTTTTATAACAAAAGAAGAATTTGAAAAACACATTCAAAATGATGATCTTTTTGAATACAGTTTTCATTTTGATAATTATTATGGAACACTTTATAGTGAAATTGCAAGAATTCATAGAAATGGCGAAATTCCTGTTTTAGAAATTGAAACCAATGGTGCAAAACAAATATTAGAAAAGGTTTCAAATAACAAGAATTACAAATTGATCACACTTTTTATCTTACCTCCTTCAATTGAAGAACTTGAAAGAAGAATTATTAACCGAAAGACAGAAAGTCAAGAAGCTATTAAAAAACGTTTAGCTAAAGCGCAAGCAGAAATTGCAGAAAAAGGAATATTTAGACATCACATAATTAATGATGACCCTTTAAGAGCTGCAAAAGAAATAAAAGAAATTATAGAGAAAGAATACGGTATCAAATAA", "aa": "MNIMNNLISKRKPIIIFTGPSGVGKGTIEKYLFDFKNLKLHLSCSATTRAPREGEINGIHYFFITKEEFEKHIQNDDLFEYSFHFDNYYGTLYSEIARIHRNGEIPVLEIETNGAKQILEKVSNNKNYKLITLFILPPSIEELERRIINRKTESQEAIKKRLAKAQAEIAEKGIFRHHIINDDPLRAAKEIKEIIEKEYGIK", "aa_hexdigest": "d2c1cadc72c5d57840c14dfdae4cea65", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF54", "query_cov": 1.0, "subject_cov": 0.9528301886792453, "identity": 1.0, "score": 397.0, "evalue": 9.73e-140, "valid": true, "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q4A6S7", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S7" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "EFPKPENHBP_1739", "locus": "GCA000186005_01732" }, { "type": "cds", "sequence": "contig_1", "start": 953025, "stop": 953249, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGGAATAACAAGCGACATGGGAAGAGTAAGGTTGGAAAACCAAGACAAAGCTAGTTACTTTACAAAAGGTAACTATGTTTTATTAATTTTATGTGATGGAATGGGTGGTCACTATGGTGGTGGGATTGCTTCAAGGACAACAATAAATACTTTTAATAAAATTTTTAACAATTCAATTCCAGCTGAAAATTCTGAAATTAAAGATTATGTATCTTGA", "aa": "MEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVS", "aa_hexdigest": "60c8b73bca8efe0ae567b04e59b7b3b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8188.092699999998, "isoelectric_point": 5.636006736755371 }, "id": "EFPKPENHBP_1740", "locus": "GCA000186005_01733" }, { "type": "cds", "sequence": "contig_1", "start": 953283, "stop": 953792, "strand": "+", "frame": 3, "gene": null, "product": "Serine/threonine phosphatase stp", "db_xrefs": [ "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_A0A449B4I9", "UniRef:UniRef90_A0A3P8KWN9" ], "nt": "ATGATTAAAATTAGTGCTAATGATGAAGCAAAACTAGACATGGGAACAACAGTTACAGCTGCCTTACTTAATACAAAAACAAAATTATTATTAATATTTAATATTGGCGATTCACGTACTTATGCTATGACAACTTTTGGTGAATTAAAGCAAATTTCAATTGATCACAACTTATACAATAAGTTAATTAATGAAGAAGGTAAAAGTCCTTATGAGGCTAAATTGAATCGTTTCCATGCTTCATTAACTTCAGCTTTAGGTCCTGATAAAAAAACTAAAATTGAAGTTTTTGACTTAAGTAGTCAATACGAACATGTTTATAGTTTATTAGTCACAAGTGATGGTGTACACGACTTTATCGAAAAACCAAAAATGGAAATGATTTTAAGGGAAAATCTTAATGCTGAAGACATGACTAAAAAATTAGTGGAAGAGGCTTTAGATAATAAATCAACAGATAACTGCTCAGCAGGTATGGTCGTATTAGACAATCTAAAAGAATGGAGTTAA", "aa": "MIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVEEALDNKSTDNCSAGMVVLDNLKEWS", "aa_hexdigest": "f142b7d75a80835e8acb645dae4916c4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 953010, "stop": 953792, "observations": { "mutation": [ 953247 ], "stop": [ 953247 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KWN9", "query_cov": 1.0, "subject_cov": 0.65, "identity": 0.9940000000000001, "score": 326.0, "evalue": 1.49e-111, "gene_start": 1, "gene_end": 169, "reference_start": 92, "reference_end": 260, "reference_sequence": "MRYQIMEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVSWFKKTVDASRDEMIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVKEALDNKSTDNCSAGMVVLDNLKEWS" }, "paralog": false, "identity": 0.9884615384615385, "score": 510.0, "evalue": 1.47e-186, "description": "Internal stop codon at 953247. Nonsense mutation around 953247.", "psc": { "uniref90_id": "UniRef90_A0A3P8KWN9", "product": "Serine/threonine phosphatase stp", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_A0A449B4I9", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KWN9", "EC:3.1.3.16", "UniRef:UniRef50_A0A449B4I9", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B4I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4I9" ], "product": "Serine/threonine phosphatase stp" } }, "id": "EFPKPENHBP_1741", "locus": "GCA000186005_01734" }, { "type": "cds", "sequence": "contig_1", "start": 953794, "stop": 954597, "strand": "+", "frame": 1, "gene": null, "product": "Protein kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5", "UniRef:UniRef90_C4XF52" ], "nt": "ATGGGAATTAAACCACAAAGTAATATATTTAAGAAATATCGTGTGCTATCAACTATTGGTGATGGTGGTTTTAGTCAAGTTTATAAAGTTGAACTTTTAAATGATAAAAGCCCAATTAAAAGAAAGTTTGCACTTAAATATAGTGTTAATAAAAATAAAAATGATGAAGACATAACTCGTAGAAGATTTGAACAAGAAATCACGATATACAAAAAATTAAATACTGAACGTGTGGCATTATACTTTGACTCTTATAGCGATGAATATGAACAATATCTTGTAATGGAATTTGTTGAAGGACAAAATTTAAGAGAAATAATTAAAAGAAATGGTAAATTCACTACAACAGTTGCTGTTAACTATGCAATTCAAATAGCTGAAGGAATTAGTGAATTACATAACTTAGGTGTAATTCACCGTGATATTAAAAGTAATAACATTTTAATTACCAAAGATAAAAATGTAAAAATTATTGACCTTGGCCTAGCTTTAGATGATGAAAGTCAAAGACTTACACAAGAAAACAAAGTAGTCGGTTCTGTTTTCTATATGGCTCCTGAAATTTGTTTAGCTAATTCGAAACCATCTGTCCGTTCGGACATTTATGCTTTAGGCATTTTGTTATTTGAAATGCTAACTGGCTCATATCCTATTTCAGGTAAAGACCAACAAGAAACTTTAAGAATGCAAAGAACAACAAATGTTCCTAACTTATTAAACTTTGTTAATGCACCTCAAGCTTTAGCTAACGTTATCATCAAAGCAACAGCTAAAGATCCAAACAAAAGATATGCAACTATGTGA", "aa": "MGIKPQSNIFKKYRVLSTIGDGGFSQVYKVELLNDKSPIKRKFALKYSVNKNKNDEDITRRRFEQEITIYKKLNTERVALYFDSYSDEYEQYLVMEFVEGQNLREIIKRNGKFTTTVAVNYAIQIAEGISELHNLGVIHRDIKSNNILITKDKNVKIIDLGLALDDESQRLTQENKVVGSVFYMAPEICLANSKPSVRSDIYALGILLFEMLTGSYPISGKDQQETLRMQRTTNVPNLLNFVNAPQALANVIIKATAKDPNKRYATM", "aa_hexdigest": "c48c69eb7c2b6a589beb00b9fb77313e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_C4XF52", "query_cov": 1.0, "subject_cov": 0.8042168674698795, "identity": 1.0, "score": 517.0, "evalue": 2.94e-184, "valid": true, "product": "Protein kinase domain-containing protein", "uniref50_id": "UniRef50_A0A449B4H5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B4H5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5" ], "product": "Serine/threonine protein kinase" }, "genes": [], "id": "EFPKPENHBP_1742", "locus": "GCA000186005_01735" }, { "type": "cds", "sequence": "contig_1", "start": 954601, "stop": 954792, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTCGTGATCTTATGACTTGTCTTGATACTAAAAGAGTTTATGAAAAACCTCTTGATCTTAGAAAAGTTAAACCTAAAAACACAATTCAAGAAAAAGTTAATAGCAAGAAATTTATTATTGCAGGTATTTCAATAATATTAGTTGTTTTGATTATAGCTTTAGTATTGATTGTTGTTTATGTTCACTAA", "aa": "MRRDLMTCLDTKRVYEKPLDLRKVKPKNTIQEKVNSKKFIIAGISIILVVLIIALVLIVVYVH", "aa_hexdigest": "8ad71a6af2053b45477d0ecbc3bec0a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.9175, "isoelectric_point": 10.008668327331542 }, "id": "EFPKPENHBP_1743", "locus": "GCA000186005_01736" }, { "type": "cds", "sequence": "contig_1", "start": 954805, "stop": 955218, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_L0RWK0", "UniRef:UniRef90_C4XF51" ], "nt": "TTGAAAGGCAAAATTTATTCAATTAATTCAGGTAAATATTACATAAATGATTTGCAAAATAAAATGCACATATTACCTGCAGCTGGTGTTTTTCGTCACAAAGAAATAACACCTTTAGTGGGTGATATTGTTGAGTTTGAAGAAGGCCAATATATCAAAGAAATATATCCTAGAAAAAATTCTTTTGTTCGTCCAAAAGTAGCAAACATCGACAATATTGTTGTGGTTATGTCAATTAAAGAACCTGAATTTCAAAGTTACTTAGTTGACAAATATTTAGCTTTTATAGAAAGTAAAAATATCAAACCAATTATCATTTTGACTAAAGATGATTTAGGTTCAACCCCTTACTTTGAACAATATAAAAATATGGGTTATGAAATTTACCAAATAAACTATAAAATAAATGATTGA", "aa": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKIND", "aa_hexdigest": "4ec913c3114f4729477e10c9da87fa97", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [ "rsgA" ], "truncated": "3-prime", "pseudogene": { "start": 954805, "stop": 955521, "observations": { "mutation": [ 955216 ], "stop": [ 955216 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF51", "query_cov": 1.0, "subject_cov": 0.49280575539568344, "identity": 1.0, "score": 279.0, "evalue": 2.7e-93, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKINDWVKIFTKIFDHKTCSLMGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE" }, "paralog": false, "identity": 0.9915966386554622, "score": 473.0, "evalue": 6.88e-172, "description": "Internal stop codon at 955216. Nonsense mutation around 955216.", "psc": { "uniref90_id": "UniRef90_C4XF51", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_L0RWK0", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF51", "EC:3.1.3.100", "EC:3.6.1.-", "UniRef:UniRef50_L0RWK0", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWK0" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "EFPKPENHBP_1744", "locus": "GCA000186005_01737" }, { "type": "cds", "sequence": "contig_1", "start": 955264, "stop": 955641, "strand": "+", "frame": 1, "gene": null, "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "nt": "ATGGGTCAAAGCGGAGTTGGAAAAACTACAATAATTAATAAATTAACCAACAATAATTATGAAACTCAAAACATTTCAAAATCAGTAAATCGGGGTAAACATACCACAAGAATTGTTCAAATAATTCCAATATTAAATGGTGAATTAATTGACACTCCAGGCTTTAGCTCTTTTGATTTAAACATCTCAAAATTAGAACTTGCTTCTTCTTATTCTCAATTTAGAGAGCTAGGAAAATTATGTAAATTTAAATCATGCTTGCATGAAAATGAACCTGAAGAATATTGCAATATAAAACTCAATGTAAAAAACAAAACAATACCAGAATTTAGATATCAAAATTATCTAAAATTATTAAAGGAGATTAAACATGAATAA", "aa": "MGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE", "aa_hexdigest": "54255a8149dd775762fd9cdb3e183f00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013679F51", "query_cov": 1.0, "subject_cov": 0.8865248226950354, "identity": 0.552, "score": 144.0, "evalue": 6.83e-42, "valid": false, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_UPI0013679F51", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013679F51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "product": "ribosome small subunit-dependent GTPase A" }, "genes": [], "id": "EFPKPENHBP_1745", "locus": "GCA000186005_01738" }, { "type": "cds", "sequence": "contig_1", "start": 955634, "stop": 955735, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAAAAATATGTAACACCTTCATTATTAAATGTTGAAAAAGATAAACGTTGTGAAATGGCAACACTATTAGTTAATAATGGAATTAAATGA", "aa": "MNKKEKYVTPSLLNVEKDKRCEMATLLVNNGIK", "aa_hexdigest": "7d565ecce1ab86c360dfabbc5514f727", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3808.4932, "isoelectric_point": 9.360438728332518 }, "id": "EFPKPENHBP_1746", "locus": "GCA000186005_01739" }, { "type": "cds", "sequence": "contig_1", "start": 955751, "stop": 956296, "strand": "+", "frame": 2, "gene": null, "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1", "UniRef:UniRef90_C4XF50" ], "nt": "ATGGATGGCAAATTTGTACCTAACACAGCTATTGAATTAGATGAAATTAAAAACATTAACAAGAATGCACCTAAACACTTTAAAGATGCTCACTTAATGGTTGAAAATCCTCTTGATTACTTAGATGAATTTAGAGACCATGTTGATATTACAACAATTCATTATGAAGCAGTTAATAAAAAAGAATTGTTGCAATTCTTAAAAGATCATCATTCAGCTTACAGTATAGGACTTGCTATTAAACCAGCAACCGAAGTTAAAGAAATAGTTAAAATACTTCCTTATTTACAACTTGTTTTAGTTATGTCTGTAGAACCTGGCAAGGGAGGACAAAAATTTATTGAACATGCTGTTGATAAAATTAGAGAACTTAAACAATTAAGAGACAAAAATGGTTACAAATATTTAATTCAAGTTGATGGTGGAATTAATGGTGAAACTGGACCAAGATGTTTTGCAGCTGGAGTAGATGCAACAGTAGCTGGAACTTATATTGTTTCAGAACCAACTAAGGAAAGAATTGCTAGTGTATTTGGTAAAAAATAA", "aa": "MDGKFVPNTAIELDEIKNINKNAPKHFKDAHLMVENPLDYLDEFRDHVDITTIHYEAVNKKELLQFLKDHHSAYSIGLAIKPATEVKEIVKILPYLQLVLVMSVEPGKGGQKFIEHAVDKIRELKQLRDKNGYKYLIQVDGGINGETGPRCFAAGVDATVAGTYIVSEPTKERIASVFGKK", "aa_hexdigest": "277d2a4d1646201ef23da892ae2f059b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF50", "query_cov": 1.0, "subject_cov": 0.8153153153153153, "identity": 1.0, "score": 366.0, "evalue": 1.31e-127, "valid": true, "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_Q4A6T1" }, "pscc": { "uniref50_id": "UniRef50_Q4A6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [], "id": "EFPKPENHBP_1747", "locus": "GCA000186005_01740" }, { "type": "cds", "sequence": "contig_1", "start": 956414, "stop": 956614, "strand": "-", "frame": 3, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354947.1", "SO:0001217", "UniParc:UPI0001E32FEB", "UniRef:UniRef100_C4XF49", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_A0A3P8MF76" ], "nt": "ATGTCAAGAAGAGACCAATTAACAGGCAAAGGACCTATGGTTGGAAACATTCGTTCACACGCAATGAATGCTTCAAAAAGAAAATTTAACGTTAACTTGCAAAAAGTAACAATGAATGTTAATGGTAAAAAAGTAACTTTAAGAGTTAGTGCTAAAACAGCAAAAACCTTAAAGAACAAAGGTGTTAGTGTTGTTGCCTAA", "aa": "MSRRDQLTGKGPMVGNIRSHAMNASKRKFNVNLQKVTMNVNGKKVTLRVSAKTAKTLKNKGVSVVA", "aa_hexdigest": "67da65a9a58e544d6c8369b3b20a907f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FEB", "ncbi_nrp_id": "WP_013354947.1", "uniref100_id": "UniRef100_C4XF49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FEB", "RefSeq:WP_013354947.1", "UniRef:UniRef100_C4XF49" ] }, "ips": { "uniref100_id": "UniRef100_C4XF49", "uniref90_id": "UniRef90_A0A3P8MF76" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MF76", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "EFPKPENHBP_1748", "locus": "GCA000186005_01741" }, { "type": "cds", "sequence": "contig_1", "start": 956875, "stop": 957258, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48", "UniRef:UniRef90_C4XF48" ], "nt": "ATGTTTAGAACTTTAAAAGAAAGATTTATAACATTAACAGTAATGTTTGCTCTTGCTTCATTAATGACTATTATAGCTATGGCATTATTTGGAGTTTCAAGAATTGTATTCACTACTTTAGGTGGTGTTACATCCTTAGGAATGTCAATAGCAGCATTTATCTTAGCAATTATAGCCTTTGTCTTTGTTCTTATTATTTACTTGCTTTACAATAGAATAATGGCAAAACGTACAGAAGATTTTAGTCGTTTTAGACACACAAAAGCATCACAAATTTTTGTTTTAGTAGCTTTAATTTGTTATGCTGTTGCAGCAGTTACAGCAATTTTGTATTTTGTAATGGTAGCTATTGTTCATAATAATAACTCAAGTACAATTAGTTGA", "aa": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIVFTTLGGVTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTIS", "aa_hexdigest": "673262d270170ab64c1d70a6fb802551", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 956722, "stop": 957258, "observations": { "mutation": [ 956811, 956832, 956877 ], "stop": [ 956811, 956832, 956877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF48", "query_cov": 1.0393700787401574, "subject_cov": 0.7213114754098361, "identity": 0.9390000000000001, "score": 204.0, "evalue": 5.22e-65, "gene_start": 1, "gene_end": 127, "reference_start": 1, "reference_end": 132, "reference_sequence": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIAFATLSGVVIGVRTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTISWETVRAFSIVFEIFFWIGIVIGWLFIILYYVFFCIIVDNITRYRRAQARKK" }, "paralog": false, "identity": 0.9398907103825137, "score": 313.0, "evalue": 1.05e-110, "description": "Internal stop codon at 956811, 956832, 956877. Nonsense mutation around 956811, 956832, 956877.", "psc": { "uniref90_id": "UniRef90_C4XF48", "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF48", "UniRef:UniRef50_C4XF48" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14112.980199999978, "isoelectric_point": 10.363308906555172 }, "id": "EFPKPENHBP_1749", "locus": "GCA000186005_01742" }, { "type": "cds", "sequence": "contig_1", "start": 957271, "stop": 958203, "strand": "-", "frame": 1, "gene": null, "product": "FAD/NAD(P)-binding domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527129.1", "SO:0001217", "UniParc:UPI0001F598DF", "UniRef:UniRef100_C4XF47", "UniRef:UniRef50_C4XF47", "UniRef:UniRef90_C4XF47" ], "nt": "ATGGACAAAAAAGATATTAAAAAATTTGATGTTTTAATTGTTGGATCAGGACCCGCAGGACTAAATGCTGCTCTTTATACCTCTCGAGCAGGACTAAATACAGCTTTTATTGATAATGATGTCTTTGGTGGCAAAATTCAAAAAACAGCTAAGGTAGATAACTACCTTGGAATTCAAGATATAAGTGGTTTTGACTTATCACAAAAATTTTACGAACATGCCTTAAAATATGGCGCAAAACATCTTTATGGCAAGGTTGTAAAAATCAATTCAATTAATGAAAATTTACAAGAAGCAATTCTTGAAAATGGTGAAATTATTCAAGCTAAAAAATTAATTATAGCTTCAGGTATGATTAATAGATATCCTAAAGGTGTTGATAATTATCAAAGACTTGAAGAATATGTAAGCTTTTGTGCTATTTGCGATGGTGCATTATATAAGAATAGAAAAGTAGTTGTATTAGGCGGCGGAAATTCTGCAGTTGAAGAAGGAACTTTTTTAGCTAAATTATGTACTGATGTAACAATAGTTACAAATCAAAATACATTTAGTGCTGAGGAAGAATTGGTTGAGGAACTCAAAAAGCAAAAAAATGCTAAAATTTATTATGATCATGAATTAATTTCTTTAAATGGTGAAAATCAGTTAACTTCAGTAACTATAAGGAATAAAAAAACCAATAAAGAAATTAATATTGTAGCTGATGGCTTATTTGTTTTCATAGGCTTTATACCAAACCAAGATGCATTTAAGCATCTAAAAATAACAACTAAAGAAGGTTTTGTAAAAACCAATGAAGATATGGAAACAAAAGTAAAAGGCATCTATGCAATTGGTGATATCAGAGATAAAAAAGTAAGACAAATTATTACAGCAGCTGCTGATGGTGCAATAGCTGCAAAACACATTTCTGATACATTAAAAAATTAG", "aa": "MDKKDIKKFDVLIVGSGPAGLNAALYTSRAGLNTAFIDNDVFGGKIQKTAKVDNYLGIQDISGFDLSQKFYEHALKYGAKHLYGKVVKINSINENLQEAILENGEIIQAKKLIIASGMINRYPKGVDNYQRLEEYVSFCAICDGALYKNRKVVVLGGGNSAVEEGTFLAKLCTDVTIVTNQNTFSAEEELVEELKKQKNAKIYYDHELISLNGENQLTSVTIRNKKTNKEINIVADGLFVFIGFIPNQDAFKHLKITTKEGFVKTNEDMETKVKGIYAIGDIRDKKVRQIITAAADGAIAAKHISDTLKN", "aa_hexdigest": "41bc8ac8a4d9758835c2e67e1bf6a022", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F598DF", "ncbi_nrp_id": "WP_013527129.1", "uniref100_id": "UniRef100_C4XF47", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598DF", "RefSeq:WP_013527129.1", "UniRef:UniRef100_C4XF47" ] }, "ips": { "uniref100_id": "UniRef100_C4XF47", "uniref90_id": "UniRef90_C4XF47" }, "psc": { "uniref90_id": "UniRef90_C4XF47", "product": "FAD/NAD(P)-binding domain-containing protein", "uniref50_id": "UniRef50_C4XF47" }, "pscc": { "uniref50_id": "UniRef50_C4XF47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF47" ], "product": "FAD/NAD(P)-binding domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1750", "locus": "GCA000186005_01743" }, { "type": "cds", "sequence": "contig_1", "start": 958319, "stop": 958444, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCATTGCGTGAAGACCAATACCACATGTATACAATAACCTCTATTGTATTTATTGGTTTAGGTGGTTTAGCTTTTGTCTTCTTTGAATTCTTCAATCCAACTCACTATGTTCGTGTTTGA", "aa": "MEPLREDQYHMYTITSIVFIGLGGLAFVFFEFFNPTHYVRV", "aa_hexdigest": "7aeeeeb78b8d297c738c663332214a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4886.6017, "isoelectric_point": 5.34811725616455 }, "id": "EFPKPENHBP_1751", "locus": "GCA000186005_01744" }, { "type": "cds", "sequence": "contig_1", "start": 958526, "stop": 958624, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAGCGATGATATTTCAAAAGGCGCTTATCGTTATCCTCTCTTCTTATATGAATCTCTTGCTAACTTAGTTGGTTACATTGTTTTAGTATGA", "aa": "MFISDDISKGAYRYPLFLYESLANLVGYIVLV", "aa_hexdigest": "194cdaf7206032b834f7b6ca8cb56998", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.260400000001, "isoelectric_point": 4.556577491760254 }, "id": "EFPKPENHBP_1752", "locus": "GCA000186005_01745" }, { "type": "cds", "sequence": "contig_1", "start": 958712, "stop": 958828, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCTTTGTACTTGACATGATTTATGTTTATACCAAAAGAGATCTAGTTGACATTCGTAAAGTTGCTTCAATTATTATTCCAACAATTCTAATTGGTCAAGTTATTGGACGTTGA", "aa": "MAFVLDMIYVYTKRDLVDIRKVASIIIPTILIGQVIGR", "aa_hexdigest": "327c3a5344c25d6e7f5528bd1bc1e2af", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4335.2679, "isoelectric_point": 9.518902015686034 }, "id": "EFPKPENHBP_1753", "locus": "GCA000186005_01746" }, { "type": "cds", "sequence": "contig_1", "start": 959142, "stop": 959465, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA", "PFAM:PF07475.17" ], "nt": "TTGGAAGCTAGAGGTATTGGTTTGATTGATATGAAATATACTTATGGAGTTAAGTCAGTAACTGATAATTGTGAAATTGATTTAGTTGTTGAATTAGTAAGTAACCAAACAGAAGAAGCAAAAAATTTAGATCGCTTAGGTCTTTCAGATCTTAAGTTTCCAATTTTAGATGGAGCTTTACCTAAGATTGAAATTCCAGTTAAAGATGGTTCAAGTGCTGCTAGCTTAATCGAAGCAGCCGTAAGTACTTTCTTAGCAAGAAAAGATGGAATCGATGTTTTAAAACAAATACAAGAGAGAGGAGAAAAAGAACATGAATTATAA", "aa": "MEARGIGLIDMKYTYGVKSVTDNCEIDLVVELVSNQTEEAKNLDRLGLSDLKFPILDGALPKIEIPVKDGSSAASLIEAAVSTFLARKDGIDVLKQIQERGEKEHEL", "aa_hexdigest": "481b267776481435217f380f925715d1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF66EA", "query_cov": 0.9065420560747663, "subject_cov": 0.9509803921568627, "identity": 0.546, "score": 103.0, "evalue": 9.78e-27, "valid": false, "uniref50_id": "UniRef50_UPI0021AF66EA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF66EA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07475.17", "name": "Hpr_kinase_C", "length": 100, "aa_cov": 0.9345794392523364, "hmm_cov": 0.98, "evalue": 3.3648606412763887e-25, "score": 84.87510681152344, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11715.26409999999, "isoelectric_point": 4.65905818939209 }, "id": "EFPKPENHBP_1754", "locus": "GCA000186005_01747" }, { "type": "cds", "sequence": "contig_1", "start": 959707, "stop": 959931, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATGTAGATGAGTTTTCAGTTGAGAAAACCGGTATTCTATATTTGTTGCAAACCTTTAAAATTCATTTTTTAGGTAACTCTCTAACCCCTTTTGAAAGAATAACTAAAGGCGGTTCTTCTTTAAGGACTCGAGCTAAAATGTTGATAGCTTCTTCTTTACCTTTTTTTGTAAAGAAAATTGATTCACTCGTACCTCAACAAATAATATTTCTTGAATAA", "aa": "MLDVDEFSVEKTGILYLLQTFKIHFLGNSLTPFERITKGGSSLRTRAKMLIASSLPFFVKKIDSLVPQQIIFLE", "aa_hexdigest": "09569d19dd4af3cdedfd82b24b598542", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.863699999998, "isoelectric_point": 9.222669792175292 }, "id": "EFPKPENHBP_1755", "locus": "GCA000186005_01748" }, { "type": "cds", "sequence": "contig_1", "start": 959907, "stop": 960083, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAAGAAAAATAAATATTAAAGAAATTATTAAGGAATTCGAACTAACAATTTGTAATGAAGAATGTGATCCTATTTACCGAGATGTTAAAGAACCTGCGATTAAAAGATTAGGTTTAGAATTATCAGGTCAAATTGAAAACACTCGTTATTCAAGAAATATTATTTGTTGA", "aa": "MRKRKINIKEIIKEFELTICNEECDPIYRDVKEPAIKRLGLELSGQIENTRYSRNIIC", "aa_hexdigest": "a3394ae51c87ee2d9643be05771a0933", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6925.045299999998, "isoelectric_point": 8.532987022399901 }, "id": "EFPKPENHBP_1756", "locus": "GCA000186005_01749" }, { "type": "cds", "sequence": "contig_1", "start": 961400, "stop": 961573, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGAAGCATTATGATAAACCATGTTTAGGCTTTTTTACCTTAGATTTTATTAAGAAAAATATAGAAGAGTTCAAACGATATAATTGCTTAGTTTTAAGTTATTTGAATCCTGATAATAACAAATATTATAGTCCTTTAAATGATCGTGAATTATTTGATTATGTATGA", "aa": "MSMKHYDKPCLGFFTLDFIKKNIEEFKRYNCLVLSYLNPDNNKYYSPLNDRELFDYV", "aa_hexdigest": "2aca2aa3ffb96819f2a44ba600bd40e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6994.953500000001, "isoelectric_point": 6.528151512145995 }, "id": "EFPKPENHBP_1757", "locus": "GCA000186005_01750" }, { "type": "cds", "sequence": "contig_1", "start": 961574, "stop": 961807, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGCTGATATTACTTTAAATAATGATTCTAAATTAAAAGAATTTAACATTACTCCTTTTTATAAATCAGGAGGAGCAAACTTCAGCGGAACAGCTGAAGCTTTATCAAAACAAAATCCTTTTCTTGATATTAAACCAAGTGAAAAAGCAGCTAAATTTTTAGAAGATACAAGTGATAAACCAAAGTATGATGTCAAATTAGAAAATGATTCAAGTAATGAATTTTGA", "aa": "MTLADITLNNDSKLKEFNITPFYKSGGANFSGTAEALSKQNPFLDIKPSEKAAKFLEDTSDKPKYDVKLENDSSNEF", "aa_hexdigest": "6ffd9497766d16633e2882933a498a2a", "start_type": "TTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8566.393699999999, "isoelectric_point": 4.791549873352051 }, "id": "EFPKPENHBP_1758", "locus": "GCA000186005_01751" }, { "type": "cds", "sequence": "contig_1", "start": 961844, "stop": 962284, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGAAAAATCTTTTGCGAGCACTTGCCCCAATTTATTCCCTACCCTTAGTTACTTTTACAACCTCTTGTAACTTTTCAGTTATTAGTTTCAAGGGTTCAAGTAGCATGATTCAGTATTTAAATGATATTTCTAAAGAATTAGGTTATGAATATGAAATGAATATTGTAGGAGGAGGCAGTGGCACTGGCCTAAGTCAATTAATTCATGGCAAAACTGATTTTGCTTCAATAAGTAAAGATCCACGTTTTGGTTTGAATTCGTCAAAGAAAAAACAAGCTAAAAAAGAATATTTAGAAAATCAACTTAAGACTTTTTTAGTTGCAAAAGAAAATGTTGCTTTTGTACTTAAACTTCCTAAATCAAAGATTCAATTATCAAGTGGAGAAGTTGAAGCAAAAGATTTAAATGTTTATTTTAACAAAAATAATTGA", "aa": "MKKKKNLLRALAPIYSLPLVTFTTSCNFSVISFKGSSSMIQYLNDISKELGYEYEMNIVGGGSGTGLSQLIHGKTDFASISKDPRFGLNSSKKKQAKKEYLENQLKTFLVAKENVAFVLKLPKSKIQLSSGEVEAKDLNVYFNKNN", "aa_hexdigest": "d59e5789d143ab08865f42e83d5e5ab5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16238.613299999986, "isoelectric_point": 9.661248207092285 }, "id": "EFPKPENHBP_1759", "locus": "GCA000186005_01752" }, { "type": "cds", "sequence": "contig_1", "start": 962757, "stop": 963260, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATATATTTAATATGGAAAATGGGACAAACTTATTTAATGCCACAATGGTCTTAAGTTTAATGTGCATTCCAACTATGATCAATTCATTATTATTGCAATTTGAAAAAGTTGATTTATGTCTAAAACTCTCAGGTAGTGCTATTGGTTTAAGTGAATCAAAAGTAATTTATAAAATAATTAAAAAGTCATTAAACAAAAAAATATTTTTAATTTATATTAGCTCTTTTGCCAGAGTTATCGGTGAATCAGCTGCTTTAATGTTTATTTTAAATAGCAATAATTATTCAGAAAATTTTACAAATATTAAGAAATTTTTTAACTCTTCATTGCGTCCTCTTTCAGTATTTATTAATGCTAATTTCTTTGCTGAAAATGGTGGCGATGCAACTAGAAGTTACATGTTTAATTTAGCTTTTGTTCTATTAATTGTGATAACTTTAATTAATGCTGCAATCATTATTATTGTTAATTTAAAAGTCAAAAAACCTAAGTGA", "aa": "MNKNIFNMENGTNLFNATMVLSLMCIPTMINSLLLQFEKVDLCLKLSGSAIGLSESKVIYKIIKKSLNKKIFLIYISSFARVIGESAALMFILNSNNYSENFTNIKKFFNSSLRPLSVFINANFFAENGGDATRSYMFNLAFVLLIVITLINAAIIIIVNLKVKKPK", "aa_hexdigest": "db0b149afdec936373812f7fe8b69273", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18722.1494, "isoelectric_point": 9.791409873962401 }, "id": "EFPKPENHBP_1760", "locus": "GCA000186005_01753" }, { "type": "cds", "sequence": "contig_1", "start": 963477, "stop": 963782, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCAACTTTAGAGCTTTATTTAACACTTTATATATCTCTTTAGTAGCAATTTTAATAACCTTCCCTTTTGCTTTTTTAGTAACAATTTATTTTGTTGAATTTAACAAAAGCAAAAAAGCAAAATTAGTATTTGATAACTTCATTTCAGTTATGACTTCAATGCCTGCTTTACTTTTTGGTGTCTTTGGTTCAGCTATCTTTTTAGGAACATTTCAATTGGCTTTAGGTGGTAAAACCAGTAACTCACTTTTAGCTGGAGCTTTAGCAATTAGCGTTATGTTATTTGCCTTCAGCTGTCGCTGA", "aa": "MGNFRALFNTLYISLVAILITFPFAFLVTIYFVEFNKSKKAKLVFDNFISVMTSMPALLFGVFGSAIFLGTFQLALGGKTSNSLLAGALAISVMLFAFSCR", "aa_hexdigest": "d563540ff53f872dd99ef91fa7232763", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11025.121799999988, "isoelectric_point": 9.899652290344235 }, "id": "EFPKPENHBP_1761", "locus": "GCA000186005_01754" }, { "type": "cds", "sequence": "contig_1", "start": 963918, "stop": 964172, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGCTAAAATCTTTTCAGAAACAACACCATTTATTTTAACTAGCGGACTAAGTAATTCAAAACATATTAGCTTATTATTATTTGGCCAAACATTAACAACTAGAATGAATGCTCAATTATCATTACCTTCTGCTTACTCAATTCCAACTATGTACTACTGTGCTATGATGTCATTTGTGACAATAGTTATCCTCTTTTCATTATCAGAATTTGTGATTCCAAAATTAAGCAAATTAAAAAGAAAACATTAG", "aa": "MLAKIFSETTPFILTSGLSNSKHISLLLFGQTLTTRMNAQLSLPSAYSIPTMYYCAMMSFVTIVILFSLSEFVIPKLSKLKRKH", "aa_hexdigest": "4dec9c3a8855e01ce8fb678273f9d50d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9449.254799999995, "isoelectric_point": 9.921055793762207 }, "id": "EFPKPENHBP_1762", "locus": "GCA000186005_01755" }, { "type": "cds", "sequence": "contig_1", "start": 964340, "stop": 964951, "strand": "+", "frame": 2, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB 3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "nt": "TTGAATCGAATTCATGGGCCACAAGCTGGAAATTATCTAGAAGGTAAAGTTGAATACTTAAATCAAGATCTCTTTAGCAAAAAATTTATTAATGATATTGAATTAAGAAAGCAAGTCGGAATGATATTTCAACAGCCAAGTGTTTTCCCTCTTTCTATTTATGATAATGTTGCTATTGGACTAAGAAATATTGGAATTAGAGATAAAAAAATATTAGACCAAAAAGTGCAAGAAGCTTTAGAAAGAGCTGCACTTTATGATGAAGTTAAAGATAAAATTAAAGAAAATGCAGAAGCATTAAGCGGTGGTCAAAAGCAAAGATTAGCTATTGCTCGAGCAATAGCGCTAAGTCCAAAAGTACTTTTAATGGATGAACCTACTAGTGCGCTAGATCCAATTGCAACTCAAAAAATAGAGAACTTAATTTTAGATTTAAAAAATGATTACACCATTATTTTAGTAACTCACTCAATGGCTCAAGCTCAGAGAGTTAGTGATAAAACTGCATTCTTTTTAGCAGGTGAATTAATTGAAATTAATAGCACTAGAAACATATTTACTAAACCACTTAAAGAAGAAACAAAAGACTACATTATGGGAAAGATAGGATAG", "aa": "MNRIHGPQAGNYLEGKVEYLNQDLFSKKFINDIELRKQVGMIFQQPSVFPLSIYDNVAIGLRNIGIRDKKILDQKVQEALERAALYDEVKDKIKENAEALSGGQKQRLAIARAIALSPKVLLMDEPTSALDPIATQKIENLILDLKNDYTIILVTHSMAQAQRVSDKTAFFLAGELIEINSTRNIFTKPLKEETKDYIMGKIG", "aa_hexdigest": "fe4c4f74239b55db94544137b65ef815", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2N8PY15", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.557, "score": 237.0, "evalue": 8.16e-76, "valid": false, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P0A2V9", "go_ids": [ "GO:0005524", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P0A2V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "product": "Phosphate import ATP-binding protein PstB 3" }, "genes": [ "pstB" ], "id": "EFPKPENHBP_1763", "locus": "GCA000186005_01756" }, { "type": "cds", "sequence": "contig_1", "start": 964956, "stop": 965174, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATAAATTACACTATATTAAAGAAGAGCGAAGATAACTTAAGAAAAATGTTTTTTGATTACTTAGACCAAGCATATCAAATGCATCTTAAATTAACTGAACTTATTTCAATATATCAAAACAAAATTGATATTGAAGGCTTATTTTTAGAAATCAATAATCGTGAAAAACAAAGCGACTTAACTCAAGTAAATACCATTGAAGAATTGCTTTGA", "aa": "MPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELL", "aa_hexdigest": "60f59d4d414ef04a68b2c46a124400e2", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8668.875899999992, "isoelectric_point": 4.781091499328612 }, "id": "EFPKPENHBP_1764", "locus": "GCA000186005_01757" }, { "type": "cds", "sequence": "contig_1", "start": 965277, "stop": 965657, "strand": "+", "frame": 3, "gene": "phoU", "product": "PhoU domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41", "UniRef:UniRef50_UPI0038737223", "UniRef:UniRef90_C4XF41" ], "nt": "ATGGCCAGATTCTTCTATCAAAACAAAAAAATTCCTCAAGAGGTTGTTAATGCTGCTTTAGCTATGAATATAGAATTAAGCAAAATTTTTAAAGAATATAGTAGCCGTCTTCAAGATGAAAAAAGTACTAAAGAATTCTTCAAAGAATTCCAAAAACAAAAAGTAGAATACAAACAATTCTGTGATAAAGAGATCAAAAAAATGGCTCGTAAATTAACCAAAAATGATGAGTATTCTAACGAAATAATTATTAAGTTTTCTTTTGAATTGCACTCATATTATCGTGTAGTTGAAAGACTAGAAAATATCATTGAAAATTTGATTTTCATTCATGATACAGATTTCTTTAGAAAGAAATTGGAAAATGAAGAAATGTTTTAA", "aa": "MARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF", "aa_hexdigest": "db0ebe688e8bd3145b04cc565b6aeec5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0038737223", "query_cov": 1.0, "subject_cov": 1.024390243902439, "identity": 0.635, "score": 158.0, "evalue": 9.57e-48, "valid": false, "uniref50_id": "UniRef50_UPI0038737223" }, "pscc": { "uniref50_id": "UniRef50_UPI0038737223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0038737223" ] }, "genes": [ "phoU" ], "truncated": "5-prime", "pseudogene": { "start": 964977, "stop": 965657, "observations": { "mutation": [ 965172, 965214 ], "stop": [ 965172, 965214 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF41", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 1.0, "score": 242.0, "evalue": 2.29e-79, "gene_start": 1, "gene_end": 126, "reference_start": 109, "reference_end": 234, "reference_sequence": "MMPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELLWDISKDQPLLSHLRWYICVLTSSGDIERICDYLYFMARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF" }, "paralog": false, "identity": 0.9911504424778761, "score": 442.0, "evalue": 6.85e-161, "description": "Internal stop codon at 965172, 965214. Nonsense mutation around 965172, 965214.", "psc": { "uniref90_id": "UniRef90_C4XF41", "product": "PhoU domain-containing protein", "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF41", "UniRef:UniRef50_C4XF41" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41" ], "product": "PhoU domain-containing protein" } }, "id": "EFPKPENHBP_1765", "locus": "GCA000186005_01758" }, { "type": "cds", "sequence": "contig_1", "start": 965866, "stop": 966765, "strand": "+", "frame": 1, "gene": null, "product": "Cytosol aminopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707", "UniRef:UniRef90_C4XF40" ], "nt": "ATGTCACTTTTTACAAAATTAGTAAATAAAGCTACTAAAGATGTTTTATTGCAAGCAACTTTTAAAGATGCTAAAAAGATTGAATATTTAATCGAAAAAAACAACGAAATTACAGAATATCTTAGTGAAAACAAAGCTTATGTTTACTTAGGAGAAGAAAGCAAGTTAGATTTTAGAACTCTTGAAAGATTTTTTAGATCTTTAAGTGAAAATAGCGCACGTAACTATTGTGTTGATTTAGCTTCATTTGCAACAAAAAATGTTTCAGTTGCTGATGTTGTTGAAGCTTTTACAAGAGCAGTTTACTTTGTTAAAGCTTCTTTATACAATGAAAAAGCTAAAGATAAAGATGCTAAAGAATTTACTTTAGTTCCTTATGTAGAAAAAGTAACTAAAGAAGTTGAAGAAAGATTAGAAAAAACATTAATTTTAGCTGAAGCTACAAATTATGCTCGTAACTTACAAGTTACACCACCAAACATTTGCAACTCAGAATATTTAGCAGACCAAATTGTTAAAGATTTCAAACAATACAAAAACTTAAAAGTTACTGTTTTAAATAAAAAACAAATTCAAGACCTTAAAATGGGATTATTACTTTCAGTTAACAGAGGGTCAGTTTATGAACCACGTGTAGTTGTTATCGAATATGCTGGTGATCCTTCAAGTAAAGAAAAAACAGTTTTAGTCGGTAAAGGTATTACTTTTGACTCAGGTGGTTACTCACTTAAGAGTCCAAGAAACATGTTAGGTATGAAATTCGACATGTCAGGTAGTGTTATTGTTGCTTCAACATTAAAAGCTATCGCACAACTTAAACCTAAGAAAAATGTTTCAGCAATTATGTGTATTACAGACAACAGAGTTAATGGTGATGCTTCATTACCTGACTCAGTTTGA", "aa": "MSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSV", "aa_hexdigest": "4c7d84cb938ae47eaed40a3780dfca4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 965836, "stop": 967068, "observations": { "mutation": [ 966763, 966940, 966961, 967006 ], "stop": [ 966763, 966940, 966961, 967006 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF40", "query_cov": 1.0, "subject_cov": 0.6402569593147751, "identity": 1.0, "score": 566.0, "evalue": 6.34e-201, "gene_start": 1, "gene_end": 299, "reference_start": 11, "reference_end": 309, "reference_sequence": "MIKLNNRRLIMSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSVWKAMNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGIWATSEKAWEDISKAADNAHELVWRMPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI" }, "paralog": false, "identity": 0.9878048780487805, "score": 772.0, "evalue": 1.47e-284, "description": "Internal stop codon at 966763, 966940, 966961, 967006. Nonsense mutation around 966763, 966940, 966961, 967006.", "psc": { "uniref90_id": "UniRef90_C4XF40", "product": "Cytosol aminopeptidase domain-containing protein", "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF40", "UniRef:UniRef50_P47707" ] }, "pscc": { "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707" ], "product": "Probable cytosol aminopeptidase" } }, "id": "EFPKPENHBP_1766", "locus": "GCA000186005_01759" }, { "type": "cds", "sequence": "contig_1", "start": 966772, "stop": 966942, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGAATGGTAAAAGCGTTGAAATCAACAACACAGATGCTGAGGGTCGTTTAGTTATGGCTGATGGTTTAGTTTATGGAGCAACCAAACTTCATGCAACACGTTTAATTGACGTTGCTACATTAACTGGTGCTATTCTTATTGCTTTAGGTCAAACCTACACAGGTATTTGA", "aa": "MNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGI", "aa_hexdigest": "6db99d060ca59c84b65830bf83bc4fd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 56, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.0909207776854606e-23, "score": 80.30740356445312, "start": 1, "stop": 56 } ], "seq_stats": { "molecular_weight": 5832.660399999999, "isoelectric_point": 5.498172187805174 }, "id": "EFPKPENHBP_1767", "locus": "GCA000186005_01760" }, { "type": "cds", "sequence": "contig_1", "start": 967012, "stop": 967239, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCATTTGATGAAGCATTCTCAAAAGGAATCAAAGCTTCTAAAGTTGCTGATCTTAAAAACACAGACTTAAGCGGTTATGGTGGATCAAGCTCAGCTGCTATGTTCTTAAAAGAATTTACAGAAGGCGTTGAATACATCCACTGTGATGTCGCAGGTACAGCAGACATTAATGAAGTGCCACAAGGTGTTTTAGTCAAAACACTTACAGAGCTTGCTTTAATTTAA", "aa": "MPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI", "aa_hexdigest": "2494b773a88588688a397ca7310615fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.92, "hmm_cov": 0.974025974025974, "evalue": 2.1663625100215883e-14, "score": 49.7672004699707, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 7940.935899999997, "isoelectric_point": 4.534353446960449 }, "id": "EFPKPENHBP_1768", "locus": "GCA000186005_01761" }, { "type": "cds", "sequence": "contig_1", "start": 967312, "stop": 967545, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTAAAACAAAGTGATGGGATTTTAGAAGTTATAACTGGCCCAATGTTCTCAGGTAAAACAGAAGAGCTTATTAAAAGAATTAAAATTTTAGAAATAGCAGATATTAAAACTTTAGTTCTTAAACCTAAATTTGATACTCGCTTTTCTGAAACAGAAATAGTAAGTAGAACAGGTGCAAAAGTTATAGCTAAAAATATTAAAAAATCAAGTGATATAATGAAATTTTGA", "aa": "MYLKQSDGILEVITGPMFSGKTEELIKRIKILEIADIKTLVLKPKFDTRFSETEIVSRTGAKVIAKNIKKSSDIMKF", "aa_hexdigest": "200ea860849e074e752178a5ca9bc592", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8732.321499999995, "isoelectric_point": 9.646098136901855 }, "id": "EFPKPENHBP_1769", "locus": "GCA000186005_01762" }, { "type": "cds", "sequence": "contig_1", "start": 967663, "stop": 967890, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCTTAAGAAGACCTTTTGGTGTAACTCCTGCAATCTTAGCAATAGCAGATCACATTACAAAGCTAAAAGCGGTATGCATGAAATGTAAATCTAGTGCAGGTTTTAGTTTTAGAAAAACAAACGAACAATCATTAAATTCGCTTGGTGATGATACTTATGAAGCTCGTTGTAGAGTCTGCCACATCAAAGGTGAACTAAAGAAAAAAGAAGACAAAAAATAA", "aa": "MDFLRRPFGVTPAILAIADHITKLKAVCMKCKSSAGFSFRKTNEQSLNSLGDDTYEARCRVCHIKGELKKKEDKK", "aa_hexdigest": "679e689a618fc70ef86a201cca496f56", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8475.851199999996, "isoelectric_point": 9.509747505187988 }, "id": "EFPKPENHBP_1770", "locus": "GCA000186005_01763" }, { "type": "cds", "sequence": "contig_1", "start": 968522, "stop": 968659, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATTTCACAATTAGATGTTGAAAGTATGAATGATTTAAGAATCAATAAAAACTTCAATACATATAATGGCGCCTTGTATGAAAATGCAATAGCTAATAGTTTATACAAACAGGACTTTAAATTATTTTATTGA", "aa": "MLISQLDVESMNDLRINKNFNTYNGALYENAIANSLYKQDFKLFY", "aa_hexdigest": "e7e701ce5ad9e6acd238915b2cca13f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5323.961100000001, "isoelectric_point": 4.8610639572143555 }, "id": "EFPKPENHBP_1771", "locus": "GCA000186005_01764" }, { "type": "cds", "sequence": "contig_1", "start": 968771, "stop": 968896, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAAGTTGAAAGAATTTATGATATGGTTGGCAAATTCTTAGGACGTGAAAACAAGAAATTTGTTTTTAATGATCTTAATTTGAATTTAAAAAGCAAACGCTGTATACCTTCTATAGACTGA", "aa": "MMKVERIYDMVGKFLGRENKKFVFNDLNLNLKSKRCIPSID", "aa_hexdigest": "2baba0b95ee0a5e4699a863967c3e6b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8009, "isoelectric_point": 9.65273838043213 }, "id": "EFPKPENHBP_1772", "locus": "GCA000186005_01765" }, { "type": "cds", "sequence": "contig_1", "start": 969280, "stop": 969480, "strand": "-", "frame": 1, "gene": null, "product": "Archaeal holliday junction resolvase (hjc)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36", "UniRef:UniRef90_C4XF36" ], "nt": "TTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGA", "aa": "MSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIA", "aa_hexdigest": "0f7b71e56878e69c8b68c51f46a7ce5f", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 969244, "stop": 969741, "observations": { "mutation": [ 969264, 969282, 969627 ], "stop": [ 969264, 969282, 969627 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF36", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.985, "score": 125.0, "evalue": 1.78e-35, "gene_start": 1, "gene_end": 66, "reference_start": 88, "reference_end": 153, "reference_sequence": "MYENFSRYNLTMKMPNYKDLNFNIKRELNKLKPKSFEEWKEAKSKVFCLALGKYLTKINSHQPDIFKNYYQVLFYHSFINYKREEELLSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIAWKESTEWKFKEI" }, "paralog": false, "identity": 0.9696969696969697, "score": 317.0, "evalue": 1.78e-113, "description": "Internal stop codon at 969264, 969282, 969627. Nonsense mutation around 969264, 969282, 969627.", "psc": { "uniref90_id": "UniRef90_C4XF36", "product": "Archaeal holliday junction resolvase (hjc)", "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF36", "UniRef:UniRef50_C4XF36" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36" ] } }, "id": "EFPKPENHBP_1773", "locus": "GCA000186005_01766" }, { "type": "cds", "sequence": "contig_1", "start": 970475, "stop": 970624, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6", "UniRef:UniRef90_UPI001004FEA6" ], "nt": "ATGTTTGAAAAAATAAAAATGAACAGATGGCGTTGCCGTTGTTGTCAAACATTAACAAATGACAAAAAAAGAGAAGTTTTTAATTACAAAAATGAAGAATATGTGTTCCCTTATTTTTGTCAAAAATGTTGTCAAAGATTAATTAATTGA", "aa": "MFEKIKMNRWRCRCCQTLTNDKKREVFNYKNEEYVFPYFCQKCCQRLIN", "aa_hexdigest": "c145cb3c93868780b3d52f69aada1530", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001004FEA6", "query_cov": 1.0, "subject_cov": 0.8909090909090909, "identity": 0.98, "score": 112.0, "evalue": 1.11e-31, "valid": true, "uniref50_id": "UniRef50_UPI001004FEA6" }, "pscc": { "uniref50_id": "UniRef50_UPI001004FEA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.3111, "isoelectric_point": 9.21532039642334 }, "id": "EFPKPENHBP_1774", "locus": "GCA000186005_01767" }, { "type": "cds", "sequence": "contig_1", "start": 970694, "stop": 970900, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTACAAAAATAAAATTGTTTTAAATAAAGAAATTGACTATGTTAAAGTTTACTTAGATCAATTTAAAATCTTAGTTGAAAGAAAACAAAAAAGTAAAAAAACTTTTCAACATTATTTAACTTTATTGAAAAGACTTAAAAGATTTGGATCGGTTGAAGAATTACTAGACCAAATGAATCATTATTTAGCTTCTTCAAATTGA", "aa": "MFYKNKIVLNKEIDYVKVYLDQFKILVERKQKSKKTFQHYLTLLKRLKRFGSVEELLDQMNHYLASSN", "aa_hexdigest": "14ae6d86cf0451ed63e4c3bddb6dc20a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8313.694899999997, "isoelectric_point": 9.776324272155762 }, "id": "EFPKPENHBP_1775", "locus": "GCA000186005_01768" }, { "type": "cds", "sequence": "contig_1", "start": 971042, "stop": 971164, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAATATTTAAAAATATTCAATTATGTTGAATTATGGGGAGATCTTAAATTCAAATTAATATTCCAAATTTTAGCTATTACAGGAGTTAGAATTGGAGAACTTGCAAATGTTAATTGA", "aa": "MEEYLKIFNYVELWGDLKFKLIFQILAITGVRIGELANVN", "aa_hexdigest": "a38e85bbfc1cd1836c1105befea360f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.5122, "isoelectric_point": 5.002479362487793 }, "id": "EFPKPENHBP_1776", "locus": "GCA000186005_01769" }, { "type": "cds", "sequence": "contig_1", "start": 971411, "stop": 971785, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGACACTTCTTTATTGTTAATGCTCATTTAAACATTGGAAGAACAGAAGAAGTTGCAAAGCTTGTTGGTCATGTTAACTCACAAACAACAGCAACAACATATTTAAACTTTAGCAATGTTGCTCTTGAAAATATTATGGAACAAGCTCAAATGGAAAATGAAAGTGCTTTTGACTATTCAAAAATTAAAATTAAATATAAAGAACTAAGCGAAGAAAATGCTCAACTCAAATTAACAATTAGTGAACTAAACAACCAATTAGACAAATACAAAAAAGTTTTAGGTGAAACAAAAGAAAATGTTAATTTTACAGAAGTATTAAATAGCAATATAAGTAAATTAAAAAATTTAATTAAAAATATTAAGTAA", "aa": "MLRHFFIVNAHLNIGRTEEVAKLVGHVNSQTTATTYLNFSNVALENIMEQAQMENESAFDYSKIKIKYKELSEENAQLKLTISELNNQLDKYKKVLGETKENVNFTEVLNSNISKLKNLIKNIK", "aa_hexdigest": "556fdf48c55ed646aa196dd5a0660b85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14263.132599999979, "isoelectric_point": 7.916024589538574 }, "id": "EFPKPENHBP_1777", "locus": "GCA000186005_01770" }, { "type": "cds", "sequence": "contig_1", "start": 971845, "stop": 972492, "strand": "-", "frame": 1, "gene": null, "product": "Mem", "db_xrefs": [ "RefSeq:WP_013526945.1", "SO:0001217", "UniParc:UPI00003B49C6", "UniRef:UniRef100_C4XDR0", "UniRef:UniRef50_Q5QGJ3", "UniRef:UniRef90_Q5QGJ3" ], "nt": "ATGAAAGATTTTTTCGCAAACCCAACTAACTTAATTATATTTTTAGGTGGTTTTGTTTCTTTAATAGCTTCTATTACTGGAATCACAACCACTTTCATTAAAACTCATAATTTAAAGCTTCAAAATAATAACCTTAAACAAGAAAATAAAGACATAAGATTTGAACTTCTTCAAACTCAAAAAGAATTGGCTGATAAAAACAATTCACTTTCAAAACTTGAAGTTATGTTTGACAACCAAAGAAAAGAAATTGAAAGACTAAGTGAAGAATTAAAAGCTTTACAAAGTGGCAATGTTGATCCTTTGGTTAAAAAGCTAAAAGAACAAATTGAGAACCTTGTTAATGAAAAGAAACAATTAACTGAACAAGTCTATAATGTTAAAGCTGAATTAAATGAGTCTAAATATCATTCTGGAGCTTATGCAAATGAAATTGTTGAATTGAAAAGGGAAAATAGTGAGCTTAAAGAGCAATTAAAGCTTGAAAGAGCTAAGATTGTTGAAGACATAGCAAATATATTAACTCCCAAAAACAATGAGGCAAATAAGGCTTCTAATGAAGCAATAAAGGAAAATAAAATAGAAGCCCCAAAAGCAGAAACCTTTGAAGCTCCAAAAAATAATGAAAATGTTAGCCTATATATTTAA", "aa": "MKDFFANPTNLIIFLGGFVSLIASITGITTTFIKTHNLKLQNNNLKQENKDIRFELLQTQKELADKNNSLSKLEVMFDNQRKEIERLSEELKALQSGNVDPLVKKLKEQIENLVNEKKQLTEQVYNVKAELNESKYHSGAYANEIVELKRENSELKEQLKLERAKIVEDIANILTPKNNEANKASNEAIKENKIEAPKAETFEAPKNNENVSLYI", "aa_hexdigest": "e970ed109eecf36c29fe7895627855cd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49C6", "ncbi_nrp_id": "WP_013526945.1", "uniref100_id": "UniRef100_C4XDR0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49C6", "RefSeq:WP_013526945.1", "UniRef:UniRef100_C4XDR0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDR0", "uniref90_id": "UniRef90_Q5QGJ3" }, "psc": { "uniref90_id": "UniRef90_Q5QGJ3", "product": "Mem", "uniref50_id": "UniRef50_Q5QGJ3" }, "pscc": { "uniref50_id": "UniRef50_Q5QGJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5QGJ3" ], "product": "Mem" }, "genes": [], "id": "EFPKPENHBP_1778", "locus": "GCA000186005_01771" }, { "type": "cds", "sequence": "contig_1", "start": 972492, "stop": 972602, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4", "UniRef:UniRef50_A0AB32XCS4", "UniRef:UniRef90_A0AB32XCS4" ], "nt": "ATGAAAATTGAAAAAAGAAATTTAGCTAAAAGAATCAAAGAAGCTTCCAAAGCTTTGAAAACAAATACATTAGACAATAAAATTGAACATGATAAGAAAGGAAACAAATAA", "aa": "MKIEKRNLAKRIKEASKALKTNTLDNKIEHDKKGNK", "aa_hexdigest": "b832ac12d69be293f91c2f306a418c97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59902", "uniref100_id": "UniRef100_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCS4", "uniref90_id": "UniRef90_A0AB32XCS4" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCS4", "uniref50_id": "UniRef50_A0AB32XCS4" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.8879000000015, "isoelectric_point": 10.170935249328611 }, "id": "EFPKPENHBP_1779", "locus": "GCA000186005_01772" }, { "type": "cds", "sequence": "contig_1", "start": 973233, "stop": 973442, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTATGCATTTCAAAATGAAAGTTTTAAAAAACTAAAAGACATTTTAGACAACTACGAAAATTTAAAGCAAGCTTTTGAATCCTTTAAAATTGCGCAAGAAAACCAAAATACTAAAAATGTCCAAATCAATTCCACACAAGATAATCAAATAAAAGCTTTAGAAGATAAAGTTGCTACTTTAGAAAATACCACATCTAATGTTTGA", "aa": "MLYAFQNESFKKLKDILDNYENLKQAFESFKIAQENQNTKNVQINSTQDNQIKALEDKVATLENTTSNV", "aa_hexdigest": "60efe6ccc3bc9e3f07fae9f50c636036", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8000.782500000001, "isoelectric_point": 4.927224540710449 }, "id": "EFPKPENHBP_1780", "locus": "GCA000186005_01773" }, { "type": "cds", "sequence": "contig_1", "start": 973476, "stop": 973679, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCATTAAAAGAATTTGTTAACAATAAAGATATTGAATTAGAAAACAAAATAAAAAGCTTGGAAGAATATGACAAATCTCAAACTCAAAATAATATTAATCAAGAAGCGCGTATTAAAAATTTAGAATATAAAACTAATGTTATTAAAAGCAAAAGCGAGATACGACTTGAAGACAATGAGGGAAAATTTCATTTATGA", "aa": "MKSLKEFVNNKDIELENKIKSLEEYDKSQTQNNINQEARIKNLEYKTNVIKSKSEIRLEDNEGKFHL", "aa_hexdigest": "b8d6947ecc42db5bd9d1dded588e4115", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7998.901199999998, "isoelectric_point": 6.583569526672363 }, "id": "EFPKPENHBP_1781", "locus": "GCA000186005_01774" }, { "type": "cds", "sequence": "contig_1", "start": 973731, "stop": 973988, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTTGTTAAAACTCAAAGTGACTCCAAAGCTATTAAAAATATTATTGGAGAAACAAGATTTGAAAACTTCAAAAAAGCTATTTATTTGGAAATTAAATATAGACTTTCAAGTAAAAGTTTTCCAGAATATTCAAATAGTGTTTTTCTGGTTTCTAATAATATTTCACTTAATGGTGAAACAAGAGATTTTGGTTCATTATCAAAATTAATAAATTATGAATCTATTCAATTATTAACATTTCCAAGTTGA", "aa": "MSYFVKTQSDSKAIKNIIGETRFENFKKAIYLEIKYRLSSKSFPEYSNSVFLVSNNISLNGETRDFGSLSKLINYESIQLLTFPS", "aa_hexdigest": "ae7805db04171dbe811287e695ce2f8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9788.01729999999, "isoelectric_point": 9.259674644470213 }, "id": "EFPKPENHBP_1782", "locus": "GCA000186005_01775" }, { "type": "cds", "sequence": "contig_1", "start": 974437, "stop": 974643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1", "UniRef:UniRef90_UPI000FEDB9C1" ], "nt": "TTGATTAAATTAGTTTGTGCTAAAAGTCCTGGAAGATACTATTTAAATAATATTGCTTATGGCCATATAGTAGGAAGATTTATTAGAAAATTTGAAATGTTTCAAGCTGTTCCAATACACCCTATTTTCTCCAATTTAATAGGAACTTCATTATTTACTTTTAGAACAATTAATTCTCTTATTATGAATTTTAAAAGACAAAAATGA", "aa": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQK", "aa_hexdigest": "bd01a20bf3fc41619262206686a58e9c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 974188, "stop": 974643, "observations": { "mutation": [ 974214, 974226, 974274, 974319, 974364, 974433, 974439 ], "stop": [ 974214, 974226, 974274, 974319, 974364, 974433, 974439 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "query_cov": 1.0, "subject_cov": 0.4503311258278146, "identity": 1.0, "score": 141.0, "evalue": 1.06e-41, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQKWVWLEDKNIILNEIVKGVKENVFKGWRAPGFQFISSMARSWATGNVGYQTKAALRWANKKAFTSKTQKTNYWVNHWVKNKYKK" }, "paralog": false, "identity": 0.9470198675496688, "score": 276.0, "evalue": 1.58e-97, "description": "Internal stop codon at 974214, 974226, 974274, 974319, 974364, 974433, 974439. Nonsense mutation around 974214, 974226, 974274, 974319, 974364, 974433, 974439.", "psc": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEDB9C1", "UniRef:UniRef50_UPI000FEDB9C1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7952.441199999995, "isoelectric_point": 10.73645191192627 }, "id": "EFPKPENHBP_1783", "locus": "GCA000186005_01776" }, { "type": "cds", "sequence": "contig_1", "start": 974812, "stop": 975294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B", "UniRef:UniRef90_UPI000FF6197B" ], "nt": "GTGACAAGAGGAATAAGATATGCAGCAAACACACATAAAACTAACAAGTTTTTTCAAATTGCAAAAAATCTTAAAAAAGAGGGTCAAGAAGAATTAAGTGAAAAAATAACAAAAATAGCTGCTAATTATTCTGAATTAGGAGTTGACAAAGCTAAATTTATTGACAATAAATCACTTTTAAAAAAAGAACTAAAATTATTTAATAATGATTCATTTTTGAGAACTTTATCTGATAACAAAAATATTTATTATTTTTTGAATAAAAAGGTTCAAAATATACAAAAAGCTTTCACACATTTAAGATCTATGGTCTCATTATTAACCTCTCCCTCATATTTAGCAAAAAAAGCAGTTGACACTGTATTTAAACACCCAAAAGCCTATTTGAACAAACAACTTGGCAAATTTACCAAATTATTAAAAAATACTTTTAAATCAAAACCAATTAAGAAAGTAGTTAAGAAAGCTTCTAATTATATTTGA", "aa": "MTRGIRYAANTHKTNKFFQIAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDNKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVDTVFKHPKAYLNKQLGKFTKLLKNTFKSKPIKKVVKKASNYI", "aa_hexdigest": "f2b10e432bf332c6d2062da8dfad3aa6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 974874, "stop": 975594, "observations": { "insertion": [ 974940 ], "mutation": [ 975339 ], "stop": [ 974909, 975339 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF6197B", "query_cov": 0.8875, "subject_cov": 0.5867768595041323, "identity": 0.8590000000000001, "score": 218.0, "evalue": 3.07e-69, "gene_start": 1, "gene_end": 142, "reference_start": 104, "reference_end": 238, "reference_sequence": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFVNTFAPGLGLVIEYGLSTALDLGFNAAINYGNINWQDFGISAGINAIPFVTRGIRYAANTHKTNKFFQLAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDQKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVALYLNTQKPIWTNNLANLQNY" }, "paralog": false, "identity": 0.9707112970711297, "score": 451.0, "evalue": 2.14e-163, "description": "Internal stop codon at 974909, 975339. Frameshift due to insertion around 974940. Nonsense mutation around 975339.", "psc": { "uniref90_id": "UniRef90_UPI000FF6197B", "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF6197B", "UniRef:UniRef50_UPI000FF6197B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18511.5323, "isoelectric_point": 10.1827974319458 }, "id": "EFPKPENHBP_1784", "locus": "GCA000186005_01777" }, { "type": "cds", "sequence": "contig_1", "start": 975337, "stop": 975603, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATTCTAACCCAAAACCAAAAACAACCTTGCAATTAAGCCCAAAATCATTTGTTATTAATGATTCAAAAAAACAAATGAATCCTTTTTTGCGAGGTTTTCTTTCATTCCTACATGTTGCTTTGGATATAGGAGCAAGTTTTTTAGGTAGTTTTATTAATACCTTTGCGCCTGGTCTTGGTCTTGTTATTGAATATGGTCTAAGTACTGCCTTAGATTTAGGTTTTAATGCTGCTATAAACTATGGAAATATCAATTGA", "aa": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFINTFAPGLGLVIEYGLSTALDLGFNAAINYGNIN", "aa_hexdigest": "babb1ec3af0953af48148397bc63a708", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9554.99199999999, "isoelectric_point": 9.157943534851075 }, "id": "EFPKPENHBP_1785", "locus": "GCA000186005_01778" }, { "type": "cds", "sequence": "contig_1", "start": 975606, "stop": 975701, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCAAAACTTTTTTGGAATAGTTTGTGATTCAAACTTTGAAGCTATTAAAAAACCAACAACTGCACCAAGTCCAAAACCTGGCTCTTAA", "aa": "MEIQNFFGIVCDSNFEAIKKPTTAPSPKPGS", "aa_hexdigest": "219b1dd5e5d2ebd9958b8645f900d938", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3354.805000000001, "isoelectric_point": 5.934922981262207 }, "id": "EFPKPENHBP_1786", "locus": "GCA000186005_01779" }, { "type": "cds", "sequence": "contig_1", "start": 975867, "stop": 976088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAAAAAAACAGATGAAGAAGTTTATAGTGAAATTGTAAACATGGCATTAAGATTAATGGAACATGAAGATTCAACATTAGGAATTGACGGAATTGAACAAAGCAAAATTGGAATTTTTGTCAAACCAAGTATTTATCAAAAAATAGTCAGATATTCAACACAAGGAAACTTTGTAAAAGAATCAATTCAAGGTAATCCTAAAGACCCAAGAACATGA", "aa": "MTKKTDEEVYSEIVNMALRLMEHEDSTLGIDGIEQSKIGIFVKPSIYQKIVRYSTQGNFVKESIQGNPKDPRT", "aa_hexdigest": "bc0ef6f55afd932b9a9a0218ba8d1c7a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8333.397099999996, "isoelectric_point": 5.746331214904786 }, "id": "EFPKPENHBP_1787", "locus": "GCA000186005_01780" }, { "type": "cds", "sequence": "contig_1", "start": 976371, "stop": 976598, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATCAAATACCAACTTTAAAACAATATTTAACTGCTGAATCTGCTACTTTATTTGAACAAGGAGCTGATAAAGCTTATACCATATCAAATGGTGGTCTAACTAAAATTTCAACTTTATTGAATGCAATTTTAGAAACAAACTTTTTAGGTAGTACATTAACTCCTGCAAGTGATCCAAATTCACCAGTTATTGAATATTTAATTTCTTTAGATCAAGAATGA", "aa": "MSYQIPTLKQYLTAESATLFEQGADKAYTISNGGLTKISTLLNAILETNFLGSTLTPASDPNSPVIEYLISLDQE", "aa_hexdigest": "8f6d975d7cc68fc30c5c7462443b62c8", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8113.025099999999, "isoelectric_point": 4.246691322326661 }, "id": "EFPKPENHBP_1788", "locus": "GCA000186005_01781" }, { "type": "cds", "sequence": "contig_1", "start": 976635, "stop": 976805, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAATTGATTTTGAGAAGATAGATGTAAATTCAATTAATCTAAATAATTTGTTTCAAAGTGTTGAGCAAGTGGCAAAAGAAAATAACATAACTATTAAGACTAAAGAACCTAATTCTGTAAATAATATTAACCAAGATTCTAGTTCTAATAGTTCTGATCCATGA", "aa": "MNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSVNNINQDSSSNSSDP", "aa_hexdigest": "478824230b7a48d6d4a4cf1a37b17b6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6281.705800000002, "isoelectric_point": 4.207586097717285 }, "id": "EFPKPENHBP_1789", "locus": "GCA000186005_01782" }, { "type": "cds", "sequence": "contig_1", "start": 976818, "stop": 977141, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6", "UniRef:UniRef90_C4XDQ6" ], "nt": "ATGCATAAAATTATTAGTGTTTTATCTAGCAAACTAGGAATTGAAGAAAGTGAACTCCTTAAAAAACTTTCATTAAATGAAAATTCTTCTCAAAAGGAGATTGCTTCTTCGCTTGGAGTTTATGAGTTTTTCCAAGAAAAAGATGACTTGACTTCATTCATCTTAAATAAAACTCAAAACCAACATAAAGATAATCAAAATTTAAAAACTGAAAATGAAAAGTTAAAAAATGATTATCAAAGTCTTGAGCAACAATTCAACACCAATTTAGAAAATTATAAAAACTTGTCCGGAAAAGTTAAAGATACTTTTAAAAATGAATGA", "aa": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNE", "aa_hexdigest": "2df2c8230f62cbfa481e4dfd77cc15a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 976611, "stop": 977141, "observations": { "mutation": [ 976637, 976820 ], "stop": [ 976637, 976820 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDQ6", "query_cov": 1.0, "subject_cov": 0.6079545454545454, "identity": 0.981, "score": 173.0, "evalue": 1.93e-53, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNEWNKYKLNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSINNINQDSSSNSSDPWSPYIRNY" }, "paralog": false, "identity": 0.9772727272727273, "score": 315.0, "evalue": 6.92e-112, "description": "Internal stop codon at 976637, 976820. Nonsense mutation around 976637, 976820.", "psc": { "uniref90_id": "UniRef90_C4XDQ6", "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDQ6", "UniRef:UniRef50_C4XDQ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12444.701699999987, "isoelectric_point": 5.738146400451661 }, "id": "EFPKPENHBP_1790", "locus": "GCA000186005_01783" }, { "type": "cds", "sequence": "contig_1", "start": 977354, "stop": 977899, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTGACCAAAAACATGAAGAATTTTTAATGTTTAATAAAGAAAAATTTAATGAAGCTTTTCAATTATCATCAATTCAAACTTCTAGATATACTATGACACCAGAAGAAGCTAGAAAAGAAATTAAAGACTTAGAACCTGGTTTAACTAAAAAGCAAATGTTTGTTCAATTAATTAAAAAAAGCGTTAAAAAAGAAATAAACATTGTTAACGCTTTTGCAAAGGTTCAATTAAACCCAACATATACAGCTAAAATTGCACATGGTGATGAACCTGGAATGATTCATTATACTTATGACACAATTCCTGCTGCATTTAAAATTGTTGATTTAGATGATTTAAATGATTGGTTAGAAGTTGTTAATTTTTCAGGTCTTGATTACAAAAATCTTGACGATAACTATGAATTAATAAATGAAACTTATGTTCCTATTTATACAATTGAATATCAAGTATATGAAAAAGAAGATTTTAAATCAAATATATATGACACTTATTTTAAGTTTCTAAACAATGATTTTATTATCAATAAAAATCAATGA", "aa": "MFIDQKHEEFLMFNKEKFNEAFQLSSIQTSRYTMTPEEARKEIKDLEPGLTKKQMFVQLIKKSVKKEINIVNAFAKVQLNPTYTAKIAHGDEPGMIHYTYDTIPAAFKIVDLDDLNDWLEVVNFSGLDYKNLDDNYELINETYVPIYTIEYQVYEKEDFKSNIYDTYFKFLNNDFIINKNQ", "aa_hexdigest": "e48a24573744bd0463c5248f7d8d07ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21492.101900000005, "isoelectric_point": 4.87601261138916 }, "id": "EFPKPENHBP_1791", "locus": "GCA000186005_01784" }, { "type": "cds", "sequence": "contig_1", "start": 978092, "stop": 978331, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATGACTGAAGAAGAAATAAAAAAATTTAAAGAGACTATTTTTGCTGGAAACAATTTAAAAGATTTTGTTGATTTTAAATTTTACACTTTGATTAACTTATATACAAAGAAAGAAAAATTTTTAAAAACTTTATTTGCAATGAGTTCAGGCAATGTTCCTTCAACATATTGTATTGATAATGGTGAAACTGAAAAACATGAACTTTATTTGCCTTTTTATTTTGATAAAAATTGA", "aa": "MGMTEEEIKKFKETIFAGNNLKDFVDFKFYTLINLYTKKEKFLKTLFAMSSGNVPSTYCIDNGETEKHELYLPFYFDKN", "aa_hexdigest": "ca3d6f91fed08138f14434c30ee4989d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9356.634399999995, "isoelectric_point": 5.774637031555175 }, "id": "EFPKPENHBP_1792", "locus": "GCA000186005_01785" }, { "type": "cds", "sequence": "contig_1", "start": 978383, "stop": 978775, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACTAGATAATAAATTTTATATTTTAGATGCTTTAAATAACCAATATGATTTGGAGCAAAAAAATAATGCTTATTCATATGGAATAGCTCAACCAATTTCTCAAATTGAAAATGAGAAAAAAATAAAATTTGTTTTCATTGAAGAAAAGCCAAATGAAATTAATGTCAGTTTAAGAACTCCATATTTGCACCAAAAAGCTTTAGAAATTGAAAACAATGGTTTAACTAGTGAATTGCCAACAAATGGCCTAGCTTTTGATATGTTTGTTAATCCAATTGAAATTAATGGTATTGACAATAAATACTATTTAAATACTTTGAGCAAATTTTCTCATTTATATTTGAATATTATTTTGAACAAAGAAAATGATTTAATGAAAAACTTTTGA", "aa": "MKLDNKFYILDALNNQYDLEQKNNAYSYGIAQPISQIENEKKIKFVFIEEKPNEINVSLRTPYLHQKALEIENNGLTSELPTNGLAFDMFVNPIEINGIDNKYYLNTLSKFSHLYLNIILNKENDLMKNF", "aa_hexdigest": "050fd94a5c6fe42199a7b8547b95ee1b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15210.12469999998, "isoelectric_point": 5.154751014709471 }, "id": "EFPKPENHBP_1793", "locus": "GCA000186005_01786" }, { "type": "cds", "sequence": "contig_1", "start": 978920, "stop": 979030, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTTATGGAATTAAAATCTTTTTTAGAGCCAAGAACTGGTGAATATATTTTAATAGACAATAAATCACTTTCTTTTGAAAATGGTTCAGTTAATGTAATATCTTGA", "aa": "MDFMELKSFLEPRTGEYILIDNKSLSFENGSVNVIS", "aa_hexdigest": "53b58b1c26164d20f808658d99ee974a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.644200000002, "isoelectric_point": 4.3588346481323255 }, "id": "EFPKPENHBP_1794", "locus": "GCA000186005_01787" }, { "type": "cds", "sequence": "contig_1", "start": 979125, "stop": 979520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTCTTAAAGACATGATCAAACAAGGACTTAAAGATCCTAATGATAGATTTATTCCAATTAATACTGCTATTCAAATGGATGGAACTCCTCCTGTTCAACTTGTGCAAGGCAATAGTCAAGCAATACCATTAATTGAAAAAATAAGATTCAATATTAAACAAATTAAAAAATTTGCTTTCTTAAAAGAAGATTCAAGTGATTCTGGAACTAAAAATATGCATAATGCTGAAGTTCAACAAATTAATAGTGATTTTGAAGATCATATTGAAGCAAAATCAAACTTAAGACAAATGCAACTATTAAACTTTTTCAAAACTTTTGAACCTAGTTTGCTAATTGATAAAGTTTTAATTTTTGGTTCAACTGAATGGCTAAAAAATGAAGCTTGA", "aa": "MNGLKDMIKQGLKDPNDRFIPINTAIQMDGTPPVQLVQGNSQAIPLIEKIRFNIKQIKKFAFLKEDSSDSGTKNMHNAEVQQINSDFEDHIEAKSNLRQMQLLNFFKTFEPSLLIDKVLIFGSTEWLKNEA", "aa_hexdigest": "0dd1a897a9693c93b3eab5c088ec459b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14978.032499999988, "isoelectric_point": 6.10345058441162 }, "id": "EFPKPENHBP_1795", "locus": "GCA000186005_01788" }, { "type": "cds", "sequence": "contig_1", "start": 979812, "stop": 979925, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCAAGTTTTAGATTATTTGCTTGATAGAAATGGTAACTTGGTAAAACTTAAAACAAGAATCAAATTTTATTCATCAAATATAAGTTTCTATATTGATGAAGAGTGA", "aa": "MPAQVLDYLLDRNGNLVKLKTRIKFYSSNISFYIDEE", "aa_hexdigest": "c5530ee6537ad677a5cf30013a1d86db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4394.997, "isoelectric_point": 6.101006507873534 }, "id": "EFPKPENHBP_1796", "locus": "GCA000186005_01789" }, { "type": "cds", "sequence": "contig_1", "start": 980058, "stop": 980189, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAACAATTATTAATAAATTAGAAAATGAAAGCAACAAATATTTTAGTTTAAGGTTTAATAATAAACCTGATTCATATAATCAATTTTTAAAATTTGGAACTCAAACAACTATTAACACTATTTGA", "aa": "MSKTIINKLENESNKYFSLRFNNKPDSYNQFLKFGTQTTINTI", "aa_hexdigest": "642a23fba149adc6f0842031d0c11584", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.690500000001, "isoelectric_point": 9.601614952087402 }, "id": "EFPKPENHBP_1797", "locus": "GCA000186005_01790" }, { "type": "cds", "sequence": "contig_1", "start": 980422, "stop": 980523, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAGTTTTTATTAACAATGGGATAAATACTAAATTGTTACCTGCTTCAAGATTTAAAGGCAAAAAAGGCTTTGGAATAATTGACAGACAAGTATGA", "aa": "MTEVFINNGINTKLLPASRFKGKKGFGIIDRQV", "aa_hexdigest": "c94b5dbbcc53bf80dbeedb9fc5400956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3693.3227000000015, "isoelectric_point": 10.446408653259276 }, "id": "EFPKPENHBP_1798", "locus": "GCA000186005_01791" }, { "type": "cds", "sequence": "contig_1", "start": 980881, "stop": 980973, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTGTTGCTGGTTTTGCTTTTGAAGAGCCACCTGAAAGATATGATTATTTTATGAAAAGTGTTTTGCTCAACAAAAATGGCAATTGA", "aa": "MITVAGFAFEEPPERYDYFMKSVLLNKNGN", "aa_hexdigest": "80388edbdc6eb996ef0a3dcc1fa895a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3481.947400000001, "isoelectric_point": 4.867827796936035 }, "id": "EFPKPENHBP_1799", "locus": "GCA000186005_01792" }, { "type": "cds", "sequence": "contig_1", "start": 980974, "stop": 981189, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAGGAACAGATTTTTTAAAGAAGATCAATTTAAATTTAGCAAAAGAATTAATTGAAAATACTTATTTGCAAAAAGAAGACTTAGCTTTTAAAGAGGGAATGGGAATTATTGTCACAATGTTTTCTAAATATTTTGTTCCTAAAAATGAATTAAGTGATTTGCAATTAAAACAATTTGAAGCTCTAAAAACTAAAGATTATAAATTATGA", "aa": "MYSGTDFLKKINLNLAKELIENTYLQKEDLAFKEGMGIIVTMFSKYFVPKNELSDLQLKQFEALKTKDYKL", "aa_hexdigest": "b97bc9f1af0001dfc0b4eb58d428ffb2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8328.668299999996, "isoelectric_point": 7.8927515029907225 }, "id": "EFPKPENHBP_1800", "locus": "GCA000186005_01793" }, { "type": "cds", "sequence": "contig_1", "start": 981508, "stop": 981852, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCTAAAAGAACCTCCTGTTTTTACAGAAGAACAATTTAAAGAATTAGTTCATTATACTGATTTAGAAGATAATCCAACTTTTGAAGCTTACAAATCTAATGCATTTTTCAAAATCTATTCAGGTGCTAAAGGTGTTTCAAAATCTTTTAGTAGAATGATTGAAACTGTTTATAGATTAGTGAATGAAAAAAATTTTAATAGTTTTTGGTGTAGAAATCAATATAACCACATTAAAGGAACATTAAAACCATTATTAGAAAAAGTTTTAAGTTTTCTTGCTGCTAAAGGTTTAGATTATAGACCTTATATTAGTATTTACAATACTGAAGCTTATTGA", "aa": "MKFLKEPPVFTEEQFKELVHYTDLEDNPTFEAYKSNAFFKIYSGAKGVSKSFSRMIETVYRLVNEKNFNSFWCRNQYNHIKGTLKPLLEKVLSFLAAKGLDYRPYISIYNTEAY", "aa_hexdigest": "aa78d02b5d28666b7ae7201407b4b445", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13488.267699999984, "isoelectric_point": 8.906130027770995 }, "id": "EFPKPENHBP_1801", "locus": "GCA000186005_01794" }, { "type": "cds", "sequence": "contig_1", "start": 982286, "stop": 982585, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526934.1", "SO:0001217", "UniParc:UPI0001F5AD4A", "UniRef:UniRef100_A0AB32XBS1", "UniRef:UniRef50_A0AB32XBS1", "UniRef:UniRef90_A0AB32XBS1" ], "nt": "TTGAATGGAATTAAAAATAATACCTATTACTCAAGCTATAACAAAGAATTTAGTGTTAAGCCAACTTGTTTTTATTCAAATATTAATTTAAATTTGAAAAACAAAAGAAAACCAGGCAATGCTGAACACTATTCAAAAGGTAGTTATTATCAACGCTCAATGATTCCAAAAGAATTAATTAAAGATTTATTTTTAAATGTTTTTGTCCAATTGAAAAAAATTTCTGTATTAAGTAAAGAATATACAAAAAACTATATTTCAAATTCACTAGAGAAAGATCAGATCTCCTTATTTGATTAA", "aa": "MNGIKNNTYYSSYNKEFSVKPTCFYSNINLNLKNKRKPGNAEHYSKGSYYQRSMIPKELIKDLFLNVFVQLKKISVLSKEYTKNYISNSLEKDQISLFD", "aa_hexdigest": "156bf1aca29644cfe6b9ec01445bd65c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5AD4A", "ncbi_nrp_id": "WP_013526934.1", "uniref100_id": "UniRef100_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD4A", "RefSeq:WP_013526934.1", "UniRef:UniRef100_A0AB32XBS1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBS1", "uniref90_id": "UniRef90_A0AB32XBS1" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBS1", "uniref50_id": "UniRef50_A0AB32XBS1" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBS1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11665.21419999999, "isoelectric_point": 9.567897987365722 }, "id": "EFPKPENHBP_1802", "locus": "GCA000186005_01795" }, { "type": "cds", "sequence": "contig_1", "start": 982643, "stop": 982789, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAACTATGAATTTTATAAAACAGTAGAAACCACAAAAAACAAAAAAAGAGATTTTTACTCTAAAGAACAAAAAAGAGTATTAGGTGAAAGTACTGCTGGAGCGACGGCTTATATAATTAATGTCTTTCAACCTAAAGTGTGA", "aa": "MNNYEFYKTVETTKNKKRDFYSKEQKRVLGESTAGATAYIINVFQPKV", "aa_hexdigest": "0ddb1aa4599f82ebcc2d737a532efb01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5637.337600000002, "isoelectric_point": 9.573055458068847 }, "id": "EFPKPENHBP_1803", "locus": "GCA000186005_01796" }, { "type": "cds", "sequence": "contig_1", "start": 982847, "stop": 983053, "strand": "-", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC", "UniRef:UniRef90_UPI0021806FCC" ], "nt": "TTGTATGATGACGGCAATATGAGTTATTTTAAAGCATTACAAAACAATGAAAATTGTGTTATTTATTCTATAGGAATACAAGAACATAAAGATTTAAAGAATTATTATAATATTGACCTTTCATTAAATAATTTTAATTTAATTGACCAACTAAAACTAATTCCAAAACCTGACATTATAATTGCTTCTCCACCTTGTGAGAGTTGA", "aa": "MYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDLSLNNFNLIDQLKLIPKPDIIIASPPCES", "aa_hexdigest": "3e37fdb238bb2c41f499446157961cd2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 982702, "stop": 983086, "observations": { "insertion": [ 982753 ], "mutation": [ 982795, 982819, 982849, 983059 ], "stop": [ 982795, 982819, 982849, 983059 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021806FCC", "query_cov": 1.0, "subject_cov": 0.5354330708661418, "identity": 0.941, "score": 136.0, "evalue": 4.9e-40, "gene_start": 1, "gene_end": 68, "reference_start": 12, "reference_end": 79, "reference_sequence": "MNENKKITIWALYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDFSLNNVNLINQLKLIPKPDIIIASPPCESWSRADCSLAIWKDISEASWKLNNYEFYKTVETKKTKSEIFTLKNKKEY" }, "paralog": false, "identity": 0.8976377952755905, "score": 223.0, "evalue": 9.25e-77, "description": "Internal stop codon at 982795, 982819, 982849, 983059. Frameshift due to insertion around 982753. Nonsense mutation around 982795, 982819, 982849, 983059.", "psc": { "uniref90_id": "UniRef90_UPI0021806FCC", "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021806FCC", "UniRef:UniRef50_UPI0021806FCC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC" ], "product": "DNA cytosine methyltransferase" } }, "id": "EFPKPENHBP_1804", "locus": "GCA000186005_01797" }, { "type": "cds", "sequence": "contig_1", "start": 983090, "stop": 983251, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526932.1", "SO:0001217", "UniParc:UPI00003B49BC", "UniRef:UniRef100_C4XDQ0", "UniRef:UniRef50_C4XDQ0", "UniRef:UniRef90_C4XDQ0" ], "nt": "ATGAATAAAGAAAAACTGATTAAATTTCTTATTGATTTAACAGAAAAAGAAAATACAAAATTAAAATCAGATGTTAAAATTCTAAAATCTAAACTTAAAAAAATAAAAAAAGAATATGAAGAACAAGAAAAAGCTTTTGATGACTTATTAAAAAAACTTTAG", "aa": "MNKEKLIKFLIDLTEKENTKLKSDVKILKSKLKKIKKEYEEQEKAFDDLLKKL", "aa_hexdigest": "b9b9b01faeae9033cd045880907e0c37", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00003B49BC", "ncbi_nrp_id": "WP_013526932.1", "uniref100_id": "UniRef100_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49BC", "RefSeq:WP_013526932.1", "UniRef:UniRef100_C4XDQ0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDQ0", "uniref90_id": "UniRef90_C4XDQ0" }, "psc": { "uniref90_id": "UniRef90_C4XDQ0", "uniref50_id": "UniRef50_C4XDQ0" }, "pscc": { "uniref50_id": "UniRef50_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.590999999999, "isoelectric_point": 9.488601875305175 }, "id": "EFPKPENHBP_1805", "locus": "GCA000186005_01798" }, { "type": "cds", "sequence": "contig_1", "start": 983414, "stop": 984268, "strand": "-", "frame": 3, "gene": null, "product": "SF4 helicase domain-containing protein", "db_xrefs": [ "EC:5.6.2.3", "KEGG:K02314", "RefSeq:WP_013526930.1", "SO:0001217", "UniParc:UPI00003B49A9", "UniRef:UniRef100_C4XDP9", "UniRef:UniRef50_C4XDP9", "UniRef:UniRef90_C4XDP9" ], "nt": "ATGCTACTCACTACAAAACAGTTACAGGAATTTTACAAAATTGAAATGGAAAATATTGCAAAAAATAAATCTCTATTAACTAACTTCAATATCATTGATAATTATGCAAATGGTTTAAAACCTAAACAACTTTATTTTTTAGCTGCAAGACCAGGCGTTGGAAAAACTTCTTTTGCTCTTAACATTATTTTTAATGCTTTGGAAAATTTGAAAGAAGATGAATGCATTGTTTTGTTTAGTCTTGAAATGGACTCAATTGAAATATATTCAAAACTTTTAGCTCTTGCCAATTCTTGTTCACTTAAAGAAGTTGAAACAAATTCTAAGAAAATAGATTTTGAAGATTTAAACAAAAAGAACCTTTTTATATTTGATAGATATAGAAACAATTTTAATAATGAAAAATTAAGTGAAGTGACTCCTGACATTATTTATAAAAGTTTGAATTCTATTGGTTTAAAGATCAAAGCTGTTTTTATTGATTATTTTCAATTGCTTGATAATCAAAAATATTCAAGTGAAAGAGAAAAGTTGTCTCAATGCTCTAAACAATTAAAAGAATTGTCAAAAATATTTAATTGTAATTTTTTTGTTTTATCTCAACTTTCAAGAGAGTATGAAAAGAAAACCAATAATGAACCATCTTTTTCTGATTTAAAAGGAACTGGTTCTATTGAACAAGACGCTGATTTAATTATGTTTCTTTATAATTCTTCAAATAATTTTAATGTTGGCACTTTAATCCCTAACAAGAAAACAATTTTGAACTTATCAATTGCAAAAAATAGAAATGGTGAATTGGTCAAAACAAATATTGATTTTATTCCGCATTTAGCAAAATTTATAGAAAGTTAA", "aa": "MLLTTKQLQEFYKIEMENIAKNKSLLTNFNIIDNYANGLKPKQLYFLAARPGVGKTSFALNIIFNALENLKEDECIVLFSLEMDSIEIYSKLLALANSCSLKEVETNSKKIDFEDLNKKNLFIFDRYRNNFNNEKLSEVTPDIIYKSLNSIGLKIKAVFIDYFQLLDNQKYSSEREKLSQCSKQLKELSKIFNCNFFVLSQLSREYEKKTNNEPSFSDLKGTGSIEQDADLIMFLYNSSNNFNVGTLIPNKKTILNLSIAKNRNGELVKTNIDFIPHLAKFIES", "aa_hexdigest": "4a93e6f8cdf2090e0fd7683161a28ac6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49A9", "ncbi_nrp_id": "WP_013526930.1", "uniref100_id": "UniRef100_C4XDP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49A9", "RefSeq:WP_013526930.1", "UniRef:UniRef100_C4XDP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XDP9", "uniref90_id": "UniRef90_C4XDP9" }, "psc": { "uniref90_id": "UniRef90_C4XDP9", "product": "SF4 helicase domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDP9", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_C4XDP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP9" ], "product": "SF4 helicase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1806", "locus": "GCA000186005_01799" }, { "type": "cds", "sequence": "contig_1", "start": 984754, "stop": 985032, "strand": "-", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8", "UniRef:UniRef50_UPI000FF29B85", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef90_UPI000FF29B85" ], "nt": "ATGAATGAAATAAAAGAATTGTTTAATAACAAAAATGTTAAATCTTATGAAGTTGATGATTTAATTAAATTTATTTCTTTTGCAAAAAGCGGTTTTGAATTTGTGCCAAATGAAACTTTAATATTTTTTGATGAAATTCAAGAACAACCTAATTGCATTACAAGTTTAAAAAGTTTTGCCTTAGACAAAAGATTTGATGTTATTTGCTCAGGTTTTTTATTAGGAATTAATTTTAAAGAACACAATATTAGACACATTGGAGTCGGTTATATTTCTTAG", "aa": "MNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS", "aa_hexdigest": "c7c822c944341a0033cd2393e79e7013", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF29B85", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 186.0, "evalue": 4.78e-60, "valid": true, "uniref50_id": "UniRef50_UPI000FF29B85" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF29B85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF29B85" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 984754, "stop": 985194, "observations": { "mutation": [ 985158 ], "stop": [ 985158 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP8", "query_cov": 1.0, "subject_cov": 0.6301369863013698, "identity": 1.0, "score": 189.0, "evalue": 3.25e-60, "gene_start": 1, "gene_end": 92, "reference_start": 55, "reference_end": 146, "reference_sequence": "MLKRKIDAFLLDWKNDANKKPLIIKGARQIGKTTSILEFAKNNYKYVVYINFALMNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS" }, "paralog": false, "identity": 0.9931506849315068, "score": 290.0, "evalue": 2.18e-102, "description": "Internal stop codon at 985158. Nonsense mutation around 985158.", "psc": { "uniref90_id": "UniRef90_C4XDP8", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef50_C4XDP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8" ], "product": "AAA domain-containing protein" } }, "id": "EFPKPENHBP_1807", "locus": "GCA000186005_01800" }, { "type": "cds", "sequence": "contig_1", "start": 985324, "stop": 985419, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGAAGTTAAACATTTTCCTATTGAACCAGAAAGTAAACAAACAATGGCTGAAATCAAAGTTTACTATGATACAAAACTAAAAAGTTAA", "aa": "MKYEVKHFPIEPESKQTMAEIKVYYDTKLKS", "aa_hexdigest": "1de529985a91ef3d671f0a9d10a6f1d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3762.3519000000006, "isoelectric_point": 8.029940223693849 }, "id": "EFPKPENHBP_1808", "locus": "GCA000186005_01801" }, { "type": "cds", "sequence": "contig_1", "start": 985648, "stop": 985803, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCAAAGTACTATTAGATTCTTAAGACCAAAAGTTAATTTATTATTAGTTGATGGGTTAGTTATCTCAGAAAATACAAGTGATTATTTTACTGATTTAATTTATAAAACAGTTCACAAGAGCTTTCTTGGTGGAGTTGAAAAAGATAAATGA", "aa": "MPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDK", "aa_hexdigest": "2300050d00982d2ac0e47781460d3841", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5827.7034, "isoelectric_point": 8.085769844055175 }, "id": "EFPKPENHBP_1809", "locus": "GCA000186005_01802" }, { "type": "cds", "sequence": "contig_1", "start": 986008, "stop": 986103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATGGTTCAGCTTATGTTTATGACGTTAATAAAGGCTTTGTTGATGATCACGCTACTGGAAAAGGCACATCAAAAGATAAGCCTAATTGA", "aa": "MIYGSAYVYDVNKGFVDDHATGKGTSKDKPN", "aa_hexdigest": "261fd1280e2c1c445ec61071177c4d8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3378.677200000001, "isoelectric_point": 6.505472755432128 }, "id": "EFPKPENHBP_1810", "locus": "GCA000186005_01803" }, { "type": "cds", "sequence": "contig_1", "start": 986131, "stop": 986865, "strand": "-", "frame": 1, "gene": "potD", "product": "PotD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4", "UniRef:UniRef90_Q6GZ00" ], "nt": "ATGAACAACAACAAACCAAAGAACAACAAATTTAAAACACTTATTAAAACCAAAATTTTAACCAAAACTTTTGGTACAAGTGCTCTTGCAGTTGTTGCTGCAGCTGCTTTAGTTGGTGCTTTATCTTATAAATATAGTAACAAAAATAACTTCAAGCCTTCATTCTACAACTATCAATCTTATATTGATCCAAATACCAAAGCAAAAATAAATAAAAACTTCAACTTCAAAGAATTTGCTGAAATTCAAGATTTTACTAAAGCTATTTTAACTAATAAAGCAGCTGCCGGAATTGGCAACGATTCTCAAGCAGTTCAACTTATTAGAGAGGGTAAACTTCGCAAAATTGATTATGCTAAAATGTTTGGTGATCCTAAATTTAATGACCGCAATGAAGTTTTAAAACTTTTAACTGACACAGTAAAAAATCATCTTGAGTCATATAACAAATATTTAACTAAAGATAAATGTATTAATGCCGATGGTTCGCAAGCTACTGAGGATCGTCACTTAATTGACTACTTTGCTCCATACTTTTCACAAGATATGGTAGTAGCTTATAATCCTGCTAAAGTTTTAAATCTTAAAAAAGATGACCCTGAATATCAAAATAAAATCAATGCTCATCAACAAGCAATGCAAAATCACCTAATTAATGAAATAAAAAACAACAAAGGTGAATTTAGACTCATTGATATTCTTAAAACATTAAAAGCTAACAAATATGAACATTGA", "aa": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEH", "aa_hexdigest": "e1bfe170e3e67bb5f4a7d5dea9992b56", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 985828, "stop": 986865, "observations": { "mutation": [ 986010, 986133 ], "stop": [ 986010, 986133 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GZ00", "query_cov": 1.0, "subject_cov": 0.4756335282651072, "identity": 1.0, "score": 476.0, "evalue": 8.75e-166, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEHWEATNAVRDNMIYGSAYVYDVNKGFVDDHATGKGTSKDKPNWYKEHIDHFVELFNHGIGYSVRDTKHINFNGDGQFLLNNLINPDSKTCAGLIYNGDAVDAYFSEDNYATVPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDKWINGPKWNSTTEEWNNIEGYESFTTFDFVGYTPAWKSQYEFVKNNYFVDDKYPQYIASLYGINKEQTIFDVDNTTPKMKYEVKHFPIEPESNQPMAEIKVYYDTKLKS" }, "paralog": false, "identity": 0.9942028985507246, "score": 696.0, "evalue": 8.94e-255, "description": "Internal stop codon at 986010, 986133. Nonsense mutation around 986010, 986133.", "psc": { "uniref90_id": "UniRef90_Q6GZ00", "product": "PotD", "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GZ00", "UniRef:UniRef50_A0A3P8K8Q4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4" ], "product": "Hypothetical lipoprotein (MG045 family)" } }, "id": "EFPKPENHBP_1811", "locus": "GCA000186005_01804" }, { "type": "cds", "sequence": "contig_1", "start": 986981, "stop": 987112, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCATCAATTGTTTTTGCTGGTTTAGTTTCCATTTTCTTATCATTTGATGACTTTATTATTTTACAAACAACTTCAAATGTAAGTACACTTGGAACCAAACTTTATGAAGGTCAATTTAAAGCTTGA", "aa": "MIPSIVFAGLVSIFLSFDDFIILQTTSNVSTLGTKLYEGQFKA", "aa_hexdigest": "583831a1e41804129e9a082136436fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4713.4439, "isoelectric_point": 4.556577491760254 }, "id": "EFPKPENHBP_1812", "locus": "GCA000186005_01805" }, { "type": "cds", "sequence": "contig_1", "start": 987134, "stop": 987271, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGAGGAATAGTAGGTCACACAATTATGGCTTTACCTTATGGTATTACTTTAATGTATCCAAGAAGTGAAAAATTCAATGCTTCATTATTTGAAGCGAGCCAAGATTTAGGTTATTCAAAAATTAGATCATGA", "aa": "MIRGIVGHTIMALPYGITLMYPRSEKFNASLFEASQDLGYSKIRS", "aa_hexdigest": "5a6f6131de25d47b54e7db228b0d492f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5063.871700000001, "isoelectric_point": 9.396283149719238 }, "id": "EFPKPENHBP_1813", "locus": "GCA000186005_01806" }, { "type": "cds", "sequence": "contig_1", "start": 987700, "stop": 988110, "strand": "-", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5", "UniRef:UniRef90_C4XDP5" ], "nt": "ATGGTTTTTGGTTTAACTTATTTAAACTTGCCATACATGATTATGCCACTTTACACAGTCTTTAAAGACATGCCTAAAAACATTCTTGAAGCTAGTGCAGACTTAGGTTACAACAAATTTCAAACATTATTTAAAGTAGTTGTTCCATATAGTTTCAAAGCTATTTTAAGTGGTTTCAGTATGATCTTCTTATCATCAGCAACCACCTTTGTTATTAGTGCAAAATTACTTCCAAATGGTACTCAATTACAAACTATTGGATCTGTAATTAATGATTATTCAAGCCCTTCAAACAAATATGAATTAGCTTTAGGAAGCACACTTGTTTTAGTTGTATCAGCCATATTTATAGGTTGTTACGGATTAATCAATTTTGTTCCTAAAATTATAATTAGACTTAAAAAAAACTAA", "aa": "MVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKN", "aa_hexdigest": "b68dbcb0c8256da2e8bc05779c125563", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 987678, "stop": 988410, "observations": { "insertion": [ 987714 ], "mutation": [ 988116, 988332, 988344 ], "stop": [ 988116, 988332, 988344 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP5", "query_cov": 1.0, "subject_cov": 0.4874551971326165, "identity": 0.993, "score": 261.0, "evalue": 5.3e-86, "gene_start": 1, "gene_end": 136, "reference_start": 137, "reference_end": 272, "reference_sequence": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGIWLIIWRSLKIGLISAILCLIIAFPYAYFIATTKSKYLAIYALSLILSPMIIFTIAKIYAIRGFFLSIFEEDSLNAEWFMVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKTKGGKKHD" }, "paralog": false, "identity": 0.9876543209876543, "score": 452.0, "evalue": 1.14e-163, "description": "Internal stop codon at 988116, 988332, 988344. Frameshift due to insertion around 987714. Nonsense mutation around 988116, 988332, 988344.", "psc": { "uniref90_id": "UniRef90_C4XDP5", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP5", "UniRef:UniRef50_C4XDP5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "EFPKPENHBP_1814", "locus": "GCA000186005_01807" }, { "type": "cds", "sequence": "contig_1", "start": 988342, "stop": 988518, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAAAAATTAAAAACAAACTTGGTTTAAATAAAAGATTACTACTTTTATTACCTTATTTATTTATTGCTATCTTTTTAATAATTTTGCCAATTTTATTAATCGTAATTAATGCTTTAAAACCACAAGATGGCTTTAATGCATCAGCTTTATTAGCTGAAAAAGGAATTTGA", "aa": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGI", "aa_hexdigest": "29f58f503bfeb1c9cc758624beca915a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6428.9259999999995, "isoelectric_point": 10.121810340881346 }, "id": "EFPKPENHBP_1815", "locus": "GCA000186005_01808" }, { "type": "cds", "sequence": "contig_1", "start": 988574, "stop": 988738, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTATGATGAAAAGAAAAAAGACAAGCTTGTTGGTGAAATTATTAACATGGCTTACCGTGGAAGTTATTACTACTTAAAAATTCAACTTGACAATGATGAAATCATTTATGTTGAAACAGCTAAAAAATTCGAAATAGGTGAAAAAGTAAACTTAAGCTGA", "aa": "MAYDEKKKDKLVGEIINMAYRGSYYYLKIQLDNDEIIYVETAKKFEIGEKVNLS", "aa_hexdigest": "eb4577405dac820bbf002ec5575ec7dc", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.2701, "isoelectric_point": 5.277580070495604 }, "id": "EFPKPENHBP_1816", "locus": "GCA000186005_01809" }, { "type": "cds", "sequence": "contig_1", "start": 988901, "stop": 989260, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "nt": "ATGGACCAAGAAGTTAAAGAAATTGTAGAATTAGTCGGACTTCAAGGTAATGAAAAAAGATCTATTAATGAACTTTCGGGTGGTATGAAACAACGTGTTGCTTTAGCCAGATCTCTTGTTATTGAACCTGCTATTCTTTTATTAGATGAACCTCTTAGTGCTCTTGATGCCAAAATTAGACAAAAAATGCAAATATTATTAAGAAGCTTACAACAAAAATTAGGTCTTACATTTATTTTTGTTACACATGATCAAGATGAAGCTCTTGAACTTTCAGATAGAATTGCAATTATGCGTGGTGGAAAAATTGAACAATATGACACGCCAAAAAATATTTATGACTATCCAGTTAATAAATGA", "aa": "MDQEVKEIVELVGLQGNEKRSINELSGGMKQRVALARSLVIEPAILLLDEPLSALDAKIRQKMQILLRSLQQKLGLTFIFVTHDQDEALELSDRIAIMRGGKIEQYDTPKNIYDYPVNK", "aa_hexdigest": "65238b594da9ee4225894c7f760cd10d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021D517A5", "query_cov": 0.9663865546218487, "subject_cov": 0.8273381294964028, "identity": 0.53, "score": 123.0, "evalue": 5.73e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021D517A5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021D517A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_1817", "locus": "GCA000186005_01810" }, { "type": "cds", "sequence": "contig_1", "start": 989366, "stop": 989458, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAGATGCTCTACAAGAAAAATATGAAAGAGAAATGGAAACATTAAAACCAAATACTTTTGCATATCGTCGTAGACAAAAATGA", "aa": "MKKLDALQEKYEREMETLKPNTFAYRRRQK", "aa_hexdigest": "84a39694df3e377086249004b9412e74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3802.3878000000013, "isoelectric_point": 9.867998313903808 }, "id": "EFPKPENHBP_1818", "locus": "GCA000186005_01811" }, { "type": "cds", "sequence": "contig_1", "start": 989480, "stop": 989875, "strand": "-", "frame": 3, "gene": null, "product": "Multiple sugar ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0", "UniRef:UniRef90_A0A3P8MEI0" ], "nt": "ATGATTAGGAAAAATAATACACCTTATATTATTGAATTAAAAGAAGTAGTTAAAGAATTCGATGATAAGGTTGTTTTGGACAATGTTGATTTAAAAATCAATAAAGGTGAATTTGTTACTTTGCTAGGACCTTCAGGATCTGGCAAAACTACAATCCTTAGACTTATTGCTGGTTTTGAAAGAGCAACACGTGGTGAAATTAAATTTCATGGTGTAGATATTAAAGACTTGCCGCCTCACAAAAGAGACCTTTCAACTATTTTTCAAGATTATGCATTATTTCCTCACTTAGATGTTGAAGGAAACATTAAATATGGTTTAGCTCTTAAAAGAAAACCAAAAGAAGTTATTAATGCTAAACATGAAAAATTATTAGTTCAAAAACAAAAACAATGA", "aa": "MIRKNNTPYIIELKEVVKEFDDKVVLDNVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLDVEGNIKYGLALKRKPKEVINAKHEKLLVQKQKQ", "aa_hexdigest": "132d9bd15df8f049e074421499d0a8cf", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 989282, "stop": 989875, "observations": { "mutation": [ 989335, 989368, 989482 ], "stop": [ 989335, 989368, 989482 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEI0", "query_cov": 0.9923664122137404, "subject_cov": 0.6372549019607843, "identity": 0.9540000000000001, "score": 246.0, "evalue": 1.55e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MIKKNNSPYIIELKEVVKEFDDKVVLENVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLNVEGNIKYGLALKRMPKEVINAKHEKLLAQKQKKWEERAKAEMKKLDVLQTKYEREMKTLKKNTFAYRRRQKWLDKSDFNYSYWENYVDQQTENFEKRYLPSLTNQK" }, "paralog": false, "identity": 0.7614213197969543, "score": 285.0, "evalue": 3e-99, "description": "Internal stop codon at 989335, 989368, 989482. Nonsense mutation around 989335, 989368, 989482.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEI0", "product": "Multiple sugar ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEI0", "UniRef:UniRef50_A0A3P8MEI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0" ], "product": "Multiple sugar ABC transporter ATP-binding protein" } }, "id": "EFPKPENHBP_1819", "locus": "GCA000186005_01812" }, { "type": "cds", "sequence": "contig_1", "start": 989996, "stop": 990148, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527148.1", "SO:0001217", "UniParc:UPI0001F59906", "UniRef:UniRef100_A0AB32XDD3", "UniRef:UniRef50_A0A168RBX0", "UniRef:UniRef90_A0AB32XDD3" ], "nt": "ATGAGAATACAAAATTTTAAGCGTGATAAATTTAAATCTAAATTTGTATTATTAAACTTTTGCGAAGATAAAAATCCAGATCTTCCATTTTTTGTTATTGAGTATTCTCAAAACGTGTTTGATTTAGCCTCACTAAGAAGTCAATCGATTTAA", "aa": "MRIQNFKRDKFKSKFVLLNFCEDKNPDLPFFVIEYSQNVFDLASLRSQSI", "aa_hexdigest": "ca5310ae2eb824651c99281c974007b1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59906", "ncbi_nrp_id": "WP_013527148.1", "uniref100_id": "UniRef100_A0AB32XDD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59906", "RefSeq:WP_013527148.1", "UniRef:UniRef100_A0AB32XDD3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDD3", "uniref90_id": "UniRef90_A0AB32XDD3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDD3", "uniref50_id": "UniRef50_A0A168RBX0" }, "pscc": { "uniref50_id": "UniRef50_A0A168RBX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RBX0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6017.886799999999, "isoelectric_point": 8.977689933776855 }, "id": "EFPKPENHBP_1820", "locus": "GCA000186005_01813" }, { "type": "cds", "sequence": "contig_1", "start": 991001, "stop": 991276, "strand": "+", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013527151.1", "SO:0001217", "UniParc:UPI0001F59909", "UniRef:UniRef100_C4XFY2", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_A0A3P8LHZ8" ], "nt": "ATGGCAAATATAAAATCAAAAGTAAAAAGCATTGCTAAGATGGAAGAATTTCGTGTACGTAACAATGCTATGAAAACAAGAGTTCGTAAAGCAATTAGAGTAGCTAGAGAAGCAATTTTAGCTAAAGATAAAAACGCTCAAGAGTTAGTTAATAAAGCTCACTCAATTATTGCTACAGCTGTTCAAAAAGGTGTTTTCCACCCAAATAAAGGTGCAAGAAAATCATCTCGTTTAGACAAATTTGTTAACGAACAAAATGCTAAAAAAGATGCATAA", "aa": "MANIKSKVKSIAKMEEFRVRNNAMKTRVRKAIRVAREAILAKDKNAQELVNKAHSIIATAVQKGVFHPNKGARKSSRLDKFVNEQNAKKDA", "aa_hexdigest": "523ee6cf391377419fa46df72ea79c4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59909", "ncbi_nrp_id": "WP_013527151.1", "uniref100_id": "UniRef100_C4XFY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59909", "RefSeq:WP_013527151.1", "UniRef:UniRef100_C4XFY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY2", "uniref90_id": "UniRef90_A0A3P8LHZ8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LHZ8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "EFPKPENHBP_1821", "locus": "GCA000186005_01814" }, { "type": "cds", "sequence": "contig_1", "start": 991338, "stop": 991481, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTTAGCACTTCAAAGAAAATTGACTCTTCCTGAATGTGCTTTAACTGACGTCTTCTTCTTGCCTCACTTTAACAAACCATTTAATTTCTTCTTAATGCCAATGCTTAAAGCACTTGGACTTAAATATAAAGCTTAA", "aa": "MFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA", "aa_hexdigest": "b0f21f40ec855f6bdf05dc44c1ed6776", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5462.648099999999, "isoelectric_point": 9.746475410461425 }, "id": "EFPKPENHBP_1822", "locus": "GCA000186005_01815" }, { "type": "cds", "sequence": "contig_1", "start": 991512, "stop": 992111, "strand": "-", "frame": 2, "gene": null, "product": "NADH oxidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1", "UniRef:UniRef90_C4XFY1" ], "nt": "ATGCAAAAAAACATGAAAAAAGAAGGTGTTAAACTTGCTCTTGGAGAAAAAGTTGTTGAATTCAAATCAAAAGATGGAAAACATGTATCACAAGTTATTACAGATAAAAGTAAATATGATGCAGATCTTGTAATTATGTCAGTAGGTTTCAAACCTAGAACATATGTATTGAATGATGTTGAAAAATTACCTAATGGAGCAATTAAAGTTAATGAATTCCAACAATCAATTTCTGACGAAAACATCTATGTTTTAGGTGATTCTGCAGCATTAAAACACTGTGTAAGCAACACATATGAACACGTAGCTTTAGCAACTAATGCAGTTAAAACAGGTATTGTAGCTGCATTAAATTTAGCTGGACTTAAAGTGCCATTTCCAGGAGTAGTAGGAACAAATGCTATTAATGTTTTTGGTTGTCACTATGCTGCAACAGGATTTAATGAAGATGCAGCTAGAGCTCATGGATTTGATGTAGCTAGTGAATATTTTGAAGACAATGACCGCTGCGAATTTGCTGGTAAAAGTGAAAAAGTTGGATGCAAAATTATTTTTGACAAGAAAACATTACAAATTTTAGGTGTTCAAATCGGATCATGA", "aa": "MQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGS", "aa_hexdigest": "f32dd76e9dbb953bbcff6925a03c8c50", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 991338, "stop": 992411, "observations": { "mutation": [ 991514, 992360 ], "stop": [ 991514, 992360 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY1", "query_cov": 1.0, "subject_cov": 0.4216101694915254, "identity": 1.0, "score": 389.0, "evalue": 1.02e-132, "gene_start": 1, "gene_end": 199, "reference_start": 216, "reference_end": 414, "reference_sequence": "MNKIKMYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAIWVGGKAKQPDGLFYSSPNILQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGTWPIEPKIPGIELDNILLSKLYQHAQVLVKKANDKKIQNVVVVGAGYIGIELVEAFHNKGKKVTLIDLADRVVPGYLDGEFTDIMQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGSWGQVNHTEVIYMFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA" }, "paralog": false, "identity": 0.9943977591036415, "score": 706.0, "evalue": 5.26e-260, "description": "Internal stop codon at 991514, 992360. Nonsense mutation around 991514, 992360.", "psc": { "uniref90_id": "UniRef90_C4XFY1", "product": "NADH oxidase", "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY1", "UniRef:UniRef50_Q4A5P1" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1" ], "product": "NADH oxidase" } }, "id": "EFPKPENHBP_1823", "locus": "GCA000186005_01816" }, { "type": "cds", "sequence": "contig_1", "start": 992358, "stop": 992507, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAAGAATATGGAGTAAATTTAAAAACTTCACATGAAGTTATCGAAATTGATAGAAAGAAAAAAGAAGTTACAGTTCAAGATTTAGATAGTGGTAAAACCTTTAAAGACACATATGATAAATTGGTTTTTGCAGGTGGTACATGA", "aa": "MQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGT", "aa_hexdigest": "41c581299c451c79791b6a3c1ee58e33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5591.2629000000015, "isoelectric_point": 6.544634819030762 }, "id": "EFPKPENHBP_1824", "locus": "GCA000186005_01817" }, { "type": "cds", "sequence": "contig_1", "start": 992562, "stop": 992741, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAAATACAAATTTTTATTACAGAAAAGAGGAAATATGAAAATTATTGTCATTGGTGCAAACCATGCAGGGACCAGCTTTTTAAGAACATTAAAAACTGTAAATCCTAAAGCAGAAATTACAGCTTATGACCGTAATACAAACACATCATTTTTAGGTTGTGGTATTGCAATTTGA", "aa": "MYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAI", "aa_hexdigest": "0f86ef6916333231590c079f92902330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6593.6998, "isoelectric_point": 10.104017066955567 }, "id": "EFPKPENHBP_1825", "locus": "GCA000186005_01818" }, { "type": "cds", "sequence": "contig_1", "start": 992965, "stop": 993516, "strand": "-", "frame": 1, "gene": null, "product": "EVE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0", "UniRef:UniRef90_C4XFY0" ], "nt": "ATGAAAAAACGTAGTCAATCATCATATGTTGACCTTAACGCTATCAAAGCATCTCTTGAACTTAAAATTAAAGAACTTGAAGACCAAAAAAATGCTTCAGATGAAACAATCAATAGCTTAAAAGAACAATTAGAAAACAAAAAATTCGACTTCATGAAAGTTGAAGAAGAAATTGAAGCTATTAAAAACCAAAAAGATCAAGAAATCAACGCAGTACGTGAAGAAGCTGAAAAGAAAATTGAAGAAGTTAAAGCAGAAGCAGCTGCAACAGCAGAACAACCAGCTTATGATCCAAATGCTGAAGTTCAATACGATCCATATGGAAACCCAATTGATCTAAATGCACAGCCAGCAATGGACCCTTATGGAAATCCATATGTACAAAGTGAAACAATTATGGCAGATCCTTATGGAACAGAAGGCGGATATTTAGTACAACCTGAAGCCTATGGGCAATTAACTCCATATGAAGCTAACTATGGATACCCAACAGGTATGTATGATGGTACTCAATTCTACCCTGTTCAACGAAAATCAGACAAAGGAATTTGA", "aa": "MKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGI", "aa_hexdigest": "90f9bd790fb7a7f1256243aa94c1a3e4", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 992869, "stop": 993816, "observations": { "mutation": [ 992967, 993789 ], "stop": [ 992967, 993789 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY0", "query_cov": 1.0, "subject_cov": 0.5111731843575419, "identity": 1.0, "score": 265.0, "evalue": 6.4e-86, "gene_start": 1, "gene_end": 183, "reference_start": 144, "reference_end": 326, "reference_sequence": "MMKRLNLLWKPKEDETYPWLLKHPKIKEGLAKFKTRQDAVDWFLNFGDEVYIWFQNNEGIFGGQLAVLESENPKDKDSKKLLSIPKVSGFDGGETYNGICEEFSIHPSYLSREPYKSALAKRIPEIDFVLISDPATYFPADLEMKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGIWVGVGIAAAVLLIILILDILVLLSLTNVAQIF" }, "paralog": false, "identity": 0.9809523809523809, "score": 513.0, "evalue": 5.45e-186, "description": "Internal stop codon at 992967, 993789. Nonsense mutation around 992967, 993789.", "psc": { "uniref90_id": "UniRef90_C4XFY0", "product": "EVE domain-containing protein", "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY0", "UniRef:UniRef50_C4XFY0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0" ], "product": "EVE domain-containing protein" } }, "id": "EFPKPENHBP_1826", "locus": "GCA000186005_01819" }, { "type": "cds", "sequence": "contig_1", "start": 994101, "stop": 994586, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGACAGAAAAAAACCAAAACGTCATTTTTATAGATGAATTAGTAAAAAAAGTTAATTTAAATGAAATCAAATTTTTCTTTTTAGAAAAGAGTACTAAACATCAAATAACAATTGAAAAGGAAATTTTAAATCAAAAGAAAATTAGTCATAATTTCGATTTCATATTAAATACTTATAATAAAATAAATAAATTATTAGATGAATCAAAAAACAATAAATGTTATTGTAACAAAATGAATCAAAACAATTTTTTAAGCAAACTTATTTTAAGGCTTGATTATTTTCCTTATGTTTTAAGAAAAGTAATCAAAACTTTAAATCCTTTTTACTTAACAAAATATCTAAAAGAAATGTGTTATTTGATAAATCAAAATCTAAAAGATAAACTTAAAAAAGATTATAGAAACTTCTATCCAATTTTAGAAGCTGTCAAAAATGTATTTGATAATGGACTAAATTTACTTGGAATAGATTTTAAAAATTAA", "aa": "MTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN", "aa_hexdigest": "519a6e33dd9a44f05299dbec9cc1006f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 109, "aa_cov": 0.6273291925465838, "hmm_cov": 1.0, "evalue": 3.550835065356642e-09, "score": 33.0975456237793, "start": 59, "stop": 159 } ], "seq_stats": { "molecular_weight": 19449.599899999997, "isoelectric_point": 9.47048625946045 }, "id": "EFPKPENHBP_1827", "locus": "GCA000186005_01820" }, { "type": "cds", "sequence": "contig_1", "start": 994731, "stop": 995732, "strand": "-", "frame": 2, "gene": null, "product": "arginine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.19", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_A0A3P8KM35", "UniRef:UniRef90_C4XFX9" ], "nt": "ATGGTTATAGTTCATAAATTGAACAAGTTAATTAAAAACATAGTAAATGAACTAAAAGCTGAAAACAAGATTTTCAGTTCTTTTAAGTTTCTTGATGGTGAAAAGAAGTTTTCACTAAAAATGGCTCGTAAAACTAAGGGCCATAACAATAATATTAACCATATTGAATGCGATATTGTTTTTCAACTTAAAAAATTTAGTTTTATTCCTTATATTGAATTAGCAGAAGAAATTAAAAAGAGATTAATCAAAGATTCAATGGTTAAAGATGTGACAATTGATCGTTATGGGCTTTTACATATTGTTTTGAATGAAAAAGATTTAGTTTCAGTGGTTAATGAAGTTATTAAGAAAGCTTATAAATATGGACAAGAAAAGAAAAATAATATTAAAGTTAATATTGAATATACTTCAGCTTCGCCACTTAATTCTTTAACTTTGATGAATGCTAGAAATGCTTTAGTTGGAGAAACTATAGCAAGAATTTTAGAATACTTAGGTTATCAAGTTACTCGAGAATTTTTAGTTTATCCAGGTTTAAAGAAAATGGAAAAAGTAAGCCAAATTGTTCATGAACAATATGTTAACTTGTTTGAATTAAATGACAATTCTATTAAAGTTGACAAGGATATTTTAGAATGTGCAAAGAATATTAAAGCACAAAATGGTGATCTTTATTTAGACATAAATGACACAATTAAAAGAAATAAATTTGATTTGGCAATTGTTTTTTATTTCTTAAATAAATCCAAAAAACAACTTAAAAATTTAGGTGTCTTTTTTGACATAGTGAGCTATTTTGAAGACATTTTGGTCAAAGAAAAGATTGACAAAGTATTAGAATTATTGTCACCTTTTATTTATGATAATGATTCAAATTTAATACTTAATACAAATCTTGATGATAGTAAAGAACGCATTCTTGTTCATAGAAATGGCACGAGTTCATATTTATTATTAGACATAATTTATCATCTTGAAAAATTAAAAAGAGCAGACTGA", "aa": "MVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRAD", "aa_hexdigest": "5e548d637e71a0b64bdabac03ce65b5f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 994428, "stop": 995762, "observations": { "mutation": [ 994733 ], "stop": [ 994733 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX9", "query_cov": 1.0, "subject_cov": 0.6021699819168174, "identity": 1.0, "score": 641.0, "evalue": 1.15e-228, "gene_start": 1, "gene_end": 333, "reference_start": 11, "reference_end": 343, "reference_sequence": "MYLKIYSGSNMVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRADWIIDILDANHTQYSHSLISALKFLNAYNNNIDIAFCENFQIKENNEVKSMTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN" }, "paralog": false, "identity": 0.9954954954954955, "score": 865.0, "evalue": 4.05e-319, "description": "Internal stop codon at 994733. Nonsense mutation around 994733.", "psc": { "uniref90_id": "UniRef90_C4XFX9", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_A0A3P8KM35", "kegg_orthology_id": "K01887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX9", "EC:6.1.1.19", "UniRef:UniRef50_A0A3P8KM35", "KEGG:K01887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM35" ], "product": "arginine--tRNA ligase" } }, "id": "EFPKPENHBP_1828", "locus": "GCA000186005_01821" }, { "type": "cds", "sequence": "contig_1", "start": 995807, "stop": 996172, "strand": "+", "frame": 2, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGTTAAAAATTTATGTCTGCGGTCCAACTGTTTATAATGATCTACATATTGGAAATATGGTTCCAATATTAACTTTTGATTTAATGCTAAAAGCTAGACGAGTTTTAGGAGAAAAATTCACTTTTGTTCACAATATTACTGATATTGATGACAAAATAATCAATCGTGCTAGAGAGCTAAATATAAGCGAAAAAGAATTAAGTCAAAAATATACTAAAGACTACTTGAAATTATTAAAAACCTTGGATGTTGACACTATAAGTCATCTTGAAAAAGTTACTGACAATATGGGCTTAATTATTGAATATGTTAATAAGCAAGTTAAAAATAATAATGCTTATATTGACCAAGAAGGTAATGTTTGA", "aa": "MLKIYVCGPTVYNDLHIGNMVPILTFDLMLKARRVLGEKFTFVHNITDIDDKIINRARELNISEKELSQKYTKDYLKLLKTLDVDTISHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNV", "aa_hexdigest": "37afea71354c967a9ddd4b884bac9645", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.9421487603305785, "subject_cov": 0.8571428571428571, "identity": 0.5, "score": 123.0, "evalue": 5.18e-34, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "EFPKPENHBP_1829", "locus": "GCA000186005_01822" }, { "type": "cds", "sequence": "contig_1", "start": 996536, "stop": 996964, "strand": "+", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC", "UniRef:UniRef90_UPI000F036BAC" ], "nt": "ATGTCAAAATCTTTAGGCAATGTTATTCTTGCAAAAGACTTTTTAAAAGAACATGATCGCGAAATTTTGAAGTTGATTATTTTGAGTTCAAAAATTTCAGCTCCAATTAATGTGACAAATGAATTAATTGAAAATATGTATGTTATTTTGAATAAATACAAAAAATTAATTTTTAAATTCTTTATTAATTTTAAAAATATTGACAAATCAAAAATTAATAAAAATGAAGAATTTATTAAGATAATGAATTCTTTAAAAATAGGCGATTTTTCTGATTTTAATTATTTATTAAATGAACAAATTAAAGAATTCAATAAAACCAAAAATATAAAAAATGCAGAAATAATTTTTAATACTTTAAAAGTTATCCACCCTCAATTAACAATTAAAGCTAACTATAACAAAGCTTTCGAGCTTTTTGACCAATGA", "aa": "MSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQ", "aa_hexdigest": "09abe47dc6b1cdc56047565e701605be", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 996236, "stop": 997042, "observations": { "mutation": [ 996287, 996347, 996497, 996962 ], "stop": [ 996287, 996347, 996497, 996962 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F036BAC", "query_cov": 1.0, "subject_cov": 0.4369230769230769, "identity": 1.0, "score": 272.0, "evalue": 1.17e-89, "gene_start": 1, "gene_end": 142, "reference_start": 158, "reference_end": 299, "reference_sequence": "LSHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNVWFDVKKFENHYGQVANLNLDKMIFEEQNNNKKFEADFALWKKTNVGIQFDSPFSKGRPGWHTECAVLINKHFKNKGLDIHGGGMDLTFPHHENENIQHFALYNQDITKEWIRCGQINYEGEKMSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQWRCFIDKKEYKQADKIRKKLMKLGVY" }, "paralog": false, "identity": 0.9850746268656716, "score": 528.0, "evalue": 5.52e-193, "description": "Internal stop codon at 996287, 996347, 996497, 996962. Nonsense mutation around 996287, 996347, 996497, 996962.", "psc": { "uniref90_id": "UniRef90_UPI000F036BAC", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F036BAC", "UniRef:UniRef50_UPI000F036BAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC" ], "product": "class I tRNA ligase family protein" } }, "id": "EFPKPENHBP_1830", "locus": "GCA000186005_01823" }, { "type": "cds", "sequence": "contig_1", "start": 997088, "stop": 997555, "strand": "+", "frame": 2, "gene": null, "product": "RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "nt": "ATGGAAAAATTATATGTGTGTGGTAGAAATTCAGTTGAAGATGCTTTAAAAGTAAACTTGCCAATTGAAACAATTTATGTCAACTCATTTCAACTCGCACAAAAATTAAAACAAATTACCAAAGCAAAAATAGAAATGAAGGATAATTCATTTTTTGCTAACTATCGTAATGATAACCATCAAGGTGTCATTGCTATTTTAAGAGACTTCCCTATTTATGATCTTGAAACAATTAAAAAAGATAATCCTGAAAATGTTTTAGTTTTAGACCACATTCAAGACCCTCATAATTTAGGTGCAATCATTAGAACTGCTAATACTTGCGGAATTAAGCATCTTATTATTAGTAAAGAAAGATCAGCTAATATTACTTCAACAGTTTTAAAAGTTAGCTCAGGTGGTTTTGTTAATATGAAAATAATTAAAGTTAATAGTATTAGTGCTACCTTAACAAAACTTAAAAAATGA", "aa": "MEKLYVCGRNSVEDALKVNLPIETIYVNSFQLAQKLKQITKAKIEMKDNSFFANYRNDNHQGVIAILRDFPIYDLETIKKDNPENVLVLDHIQDPHNLGAIIRTANTCGIKHLIISKERSANITSTVLKVSSGGFVNMKIIKVNSISATLTKLKK", "aa_hexdigest": "441e77b1e4b25f25cf0d3eb7a60482b8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0011424F0F", "query_cov": 1.0129032258064516, "subject_cov": 0.8440860215053764, "identity": 0.535, "score": 157.0, "evalue": 2.9e-46, "valid": false, "product": "RNA methyltransferase", "uniref50_id": "UniRef50_UPI0011424F0F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011424F0F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "product": "RNA methyltransferase" }, "genes": [], "id": "EFPKPENHBP_1831", "locus": "GCA000186005_01824" }, { "type": "cds", "sequence": "contig_1", "start": 997785, "stop": 998378, "strand": "+", "frame": 3, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "RefSeq:WP_013354970.1", "SO:0001217", "UniParc:UPI0001E33011", "UniRef:UniRef100_A0AB32XDA1", "UniRef:UniRef50_C4XFX6", "UniRef:UniRef90_C4XFX6" ], "nt": "ATGAAATATTTACAAAAATTTTTAGACTTACATCAAACACCCACGCAAGAGTTTGATGAATTTTTACATAGCTTAAAAGACAATCAATTGATTATGATATTGGATTATTTTTACAAAAATGAATTTATCAAAAATATCAAAAGCACTTTAATTAGATTTCCTTATATTCCATTAGAAGCTGAAGATATTTATATTGAATTTTTGCAAACCTATTTAGAAGAAGTTAAAAAGTATAATTCAACTGACAAAAATGTTAAATTTTTAAATTTCTTTTTAAATATCTCCAAATTTTACACATTAAATAAAATTAGATATTGGCTTAGAAAAAAAAGAATTCATAACTCACTTATGACTTCAACTGATGAATTGTTATATGTTTTAGATGAGCATTCTGAAGAACAAATTGAACAAAGAATTAATCAAATTGATACTGAAAACTTTTATCATTTATTAACTGACAAAGATAAAAATATAATTAAAATATTGCAAAATAGTATCAATCAAAAGGACAAATTGATTACACCTAGTAAACTAAAAGAATTTAAAACTAAATTTTTAACTAAATTTAATAATTATTTTCATTTTGCCCATTAA", "aa": "MKYLQKFLDLHQTPTQEFDEFLHSLKDNQLIMILDYFYKNEFIKNIKSTLIRFPYIPLEAEDIYIEFLQTYLEEVKKYNSTDKNVKFLNFFLNISKFYTLNKIRYWLRKKRIHNSLMTSTDELLYVLDEHSEEQIEQRINQIDTENFYHLLTDKDKNIIKILQNSINQKDKLITPSKLKEFKTKFLTKFNNYFHFAH", "aa_hexdigest": "28292666f9e5d3f0def93393b654e5de", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E33011", "ncbi_nrp_id": "WP_013354970.1", "uniref100_id": "UniRef100_A0AB32XDA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33011", "RefSeq:WP_013354970.1", "UniRef:UniRef100_A0AB32XDA1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDA1", "uniref90_id": "UniRef90_C4XFX6" }, "psc": { "uniref90_id": "UniRef90_C4XFX6", "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFX6" }, "pscc": { "uniref50_id": "UniRef50_C4XFX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX6" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "EFPKPENHBP_1832", "locus": "GCA000186005_01825" }, { "type": "cds", "sequence": "contig_1", "start": 998865, "stop": 999116, "strand": "+", "frame": 3, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_A0A4Y6I615", "UniRef:UniRef90_C4XFX3" ], "nt": "ATGATTAGTACAGTTTCTGGAAAAGAAGATAAGGTTGTTGAAGCTTTAAAAAATAGAATTATTAGTGAACAAGTTGAAGATTGCTTTAATCATGAAGCAACACCTGAAGGCGCTTTTAAAATTTTCAAAAAACCAACCTTAACACCTAAAGAATTAGAAAAACAAAAAAATGGTGAAGAATACAAAGTTAAATATGTAAACATGTATTCAGGCTACATTTTTATTAATATGGACATGACAGATAGAGCTTGA", "aa": "MISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRA", "aa_hexdigest": "ed73095dc91506e0802b5751155f2adc", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "both", "pseudogene": { "start": 998829, "stop": 999419, "observations": { "mutation": [ 998859, 999114 ], "stop": [ 998859, 999114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX3", "query_cov": 1.0, "subject_cov": 0.40487804878048783, "identity": 1.0, "score": 168.0, "evalue": 1.66e-51, "gene_start": 1, "gene_end": 83, "reference_start": 13, "reference_end": 95, "reference_sequence": "MEDIMDKNFQWYMISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRAWFIVRNTQYVTGLIGSSGRGAKPTPVSQHQIDKSFKKEIKLQEDFDKGVTHTKFQIGEIVEIIDGPFKGEQGRVLECSEANKKVTVEIEYLGKKTPADFEYKEIKSQEYE" }, "paralog": false, "identity": 0.9846938775510204, "score": 388.0, "evalue": 1.35e-140, "description": "Internal stop codon at 998859, 999114. Nonsense mutation around 998859, 999114.", "psc": { "uniref90_id": "UniRef90_C4XFX3", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_A0A4Y6I615", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX3", "UniRef:UniRef50_A0A4Y6I615", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I615", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I615" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "EFPKPENHBP_1833", "locus": "GCA000186005_01826" }, { "type": "cds", "sequence": "contig_1", "start": 999591, "stop": 1000250, "strand": "-", "frame": 2, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_D3VPP6", "UniRef:UniRef90_C4XFX2" ], "nt": "ATGAGCTATAGAAAAATTACTAAAAAAGATTTACTTGAAAAATATTCTTCAAAAATTAGCATATTAAAAGAATATGCTCAAATGATTTATGATCAAAATCAAGTTATGAATATCACAGGTTTTAAAACACTAGATGATATTTTTGAACAAGGTATACTTGATTCTATTTTAGCTTTTGAAAATTACATGATGCATTATGATGAAAATCTAGAAAACAAAAAAATATTAGATGTAGGTGCCGGTGCAGGATTTCCTTCAATTCCAATTCTAATTGCATTAGATAATAAGTTCAATTTAACTATTATTGAATCTCAAAAGAAAAGATGCAATTTTTTAGAAAAAGTTAAAGCACGATTTAATTTAAGTGTCAATATAATTAATTCAAGAGTTGAAGAGTTTTTTAAATTAACAAATTACTTTGAGATTATTACTGCTCGAGCTGTTGCAAGCATTAAAGTTTTATATTTAAGTGCAGCGCACTTATTAAAAAACAGAGGAAAATTTGTTTTATTAAAAGGCGAAAACTTTAGAGATGAGCTTACTGATATTGTAAATATTGCTAAATACATGGGCGAAATGGGCGGCTTTGAATATGATAATGTTTTAAATGAAACGTCTAGCGTGGTGGTTATTAACAAAGGAAAAATTCCTGATGATTGA", "aa": "MSYRKITKKDLLEKYSSKISILKEYAQMIYDQNQVMNITGFKTLDDIFEQGILDSILAFENYMMHYDENLENKKILDVGAGAGFPSIPILIALDNKFNLTIIESQKKRCNFLEKVKARFNLSVNIINSRVEEFFKLTNYFEIITARAVASIKVLYLSAAHLLKNRGKFVLLKGENFRDELTDIVNIAKYMGEMGGFEYDNVLNETSSVVVINKGKIPDD", "aa_hexdigest": "8aa655d6e513edef9385f9978c75a92d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX2", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 1.0, "score": 425.0, "evalue": 4.05e-150, "valid": true, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_D3VPP6", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPP6" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "EFPKPENHBP_1834", "locus": "GCA000186005_01827" }, { "type": "cds", "sequence": "contig_1", "start": 1000250, "stop": 1001230, "strand": "-", "frame": 3, "gene": null, "product": "Ribose-phosphate diphosphokinase", "db_xrefs": [ "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_013354975.1", "SO:0001217", "UniParc:UPI0001E33016", "UniRef:UniRef100_A0AB32XD72", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_A0A3P8K8X3" ], "nt": "ATGGATAAAAAAAATGTAATGCTTTTTGGTATGCCGAATAGCTTGAAATTAGCAGAAAAAATTTCAAAAATTATTAAAGTTCCTTTGACAGGAATTACTAAAACAACTTTTGCTGATGGCGAAGTTATGTTAGTTAGCGACAAAACAGTTAGAGAAAAAGATGTTTTCGTTATTGCTTCAACATCAAGACCAGTTAATGAAAACTTAATGGAGTTATTCTTATTTATTGACTCGCTTAAAAGAGCAAGTGTTAAAAGTGTTACAGTTGCTTTAAGTTACTATGGATATGCACGTCAAGATCGCAAAGCAAGTGGACGTCAACCTATTGGTGCAAAACTAGTAGCAGACTTATTACAAACAGCGGGTGCTGACAAAGTTATTGCAGTTGATTTACATAACCCTGCAATTCAAGGTTTCTTCAATATTCCTATTGATGATTTGCGTGGTGCTTATACTTTAGCAAAAGCTATTAAAAATACACATGAATCATTTACTGTTGTTTCACCTGACCACGGTGGAACAATTAGAGCAAGAAAACTTGCTGAATTAATTTCAAACGATGTAAAAATTTGTATTATTGACAAACGTCGTATTGGCACAAACCAAACTGAAGTTTTAGGACTTATTGGTGATATTGAAGGTCAAAATGCAGTTATCATTGATGATATCATTGATACTGGAGGAACAATTTTAAAAGCAGTTGATACATTAAAAGCACACGGTGCAAAGAAAATTATTGTAGCTGCAACTCACGGTATTTTTACAAAAGGATTTGAAATTTTTGAAAATAATCCAAATGTTTATAAAGTAATTATTACTGATTCAATTGAAAATGCAGAATTAAGAGATGGTAAATTCAAAAAACTCGAAGTAATAAGTCTTGACAGATTCCTAGCTAATGTTATTGAATCATCAATTAGTGGTAAATCTATTTCTCATTTATATCAACAATTTGCAAAAGAAATTACTTCAAATAAATAA", "aa": "MDKKNVMLFGMPNSLKLAEKISKIIKVPLTGITKTTFADGEVMLVSDKTVREKDVFVIASTSRPVNENLMELFLFIDSLKRASVKSVTVALSYYGYARQDRKASGRQPIGAKLVADLLQTAGADKVIAVDLHNPAIQGFFNIPIDDLRGAYTLAKAIKNTHESFTVVSPDHGGTIRARKLAELISNDVKICIIDKRRIGTNQTEVLGLIGDIEGQNAVIIDDIIDTGGTILKAVDTLKAHGAKKIIVAATHGIFTKGFEIFENNPNVYKVIITDSIENAELRDGKFKKLEVISLDRFLANVIESSISGKSISHLYQQFAKEITSNK", "aa_hexdigest": "8bda781252f5dfa72b5917a58f1def57", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E33016", "ncbi_nrp_id": "WP_013354975.1", "uniref100_id": "UniRef100_A0AB32XD72", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33016", "RefSeq:WP_013354975.1", "UniRef:UniRef100_A0AB32XD72" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD72", "uniref90_id": "UniRef90_A0A3P8K8X3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8X3", "product": "Ribose-phosphate diphosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [], "id": "EFPKPENHBP_1835", "locus": "GCA000186005_01828" }, { "type": "cds", "sequence": "contig_1", "start": 1001411, "stop": 1002526, "strand": "+", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_C4XFX0" ], "nt": "ATGCTTTTTAAAAATGAATATTTTTATATAATTATAAACATGGAAAAAAATATGTTCAATTCGTTGTCTGATATTAAAAATACCTATGACAATTTAGTTAAAAAATTGGATGATCATGAAGTTATCAATAACATCAAGGAATACACAAAAATAAGTAAAGAAGTCAACAAAATTAAGGATATAGCAGAAACCTTTACTAAGTACTTAAATTTACAAAATGACTACAATGATGCAAAAGTAATGATCAATTCAAAAAATGAAGAGGAAGTTCTTTTTGCTAAAAGTATCATTGAAGAAAATGAAGTTAAATTAGCACAACTTGAAGAAGAATTAAAAATTTTAATTCTGCCTAAAGATGAAAACGATGACAAGAATGTTATTATGGAAATTAGAGGTGCAGCAGGCGGTGACGAAGCTAATATTTTTGCTGGCGACTTATTCAAAATGTACACAAAATTTGCAGAAGATTTAAATTTCAAATTAAAAGTTATATCTTCTTCAGCAGCTAGTGTTGGTGGTTTTACTCAAATAGTATTTAGTATTAAGGGTGATAAAGCTTATTCAAAACTTAAGTTTGAAAGTGGAGTGCATCGTGTGCAACGCGTACCTGTAACTGAAAGTGCAGGTAGAATTCATACTTCAACAGCAACAGTTACTGTTATGCCTGAAATTGATGATAGTGTTGATATTGAAATCAAACCACAAGATTTACAAATTGATACATATAGATCATCTGGAGCTGGTGGCCAATCAGTTAATACAACAGACTCAGCGGTTAGAATTACACACTTGCCTACTGGTATAGTTGTTTCATCGCAAGATGAAAGAAGTCAAATAGCAAATAGAGAAACTGCTTTAACAGTTCTTAAAAGTAAGCTTTATGATTTAGAAATTCAAAAGAAACAACAAGAAGAAGCAGGGTATAGAAAACTAGCAGGTCATGGTGATCGGAGCGAAAAAATTAGAACATATAACTATCCACAAGATAGAGTTACAGACCATAGAATTGGATATTCAACAAGTTTAAAACAAGTAATGGAAGGTAAATTATTAACTATTATTGAAGCATTACTAACAGAAGAACAAAATCAAAAAATAAAGGAAAGTGGCTTATAA", "aa": "MLFKNEYFYIIINMEKNMFNSLSDIKNTYDNLVKKLDDHEVINNIKEYTKISKEVNKIKDIAETFTKYLNLQNDYNDAKVMINSKNEEEVLFAKSIIEENEVKLAQLEEELKILILPKDENDDKNVIMEIRGAAGGDEANIFAGDLFKMYTKFAEDLNFKLKVISSSAASVGGFTQIVFSIKGDKAYSKLKFESGVHRVQRVPVTESAGRIHTSTATVTVMPEIDDSVDIEIKPQDLQIDTYRSSGAGGQSVNTTDSAVRITHLPTGIVVSSQDERSQIANRETALTVLKSKLYDLEIQKKQQEEAGYRKLAGHGDRSEKIRTYNYPQDRVTDHRIGYSTSLKQVMEGKLLTIIEALLTEEQNQKIKESGL", "aa_hexdigest": "ca45339f46f8993cc611d5a44d19a4dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX0", "query_cov": 1.0, "subject_cov": 0.9946380697050938, "identity": 1.0, "score": 692.0, "evalue": 4.14e-251, "valid": true, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "EFPKPENHBP_1836", "locus": "GCA000186005_01829" }, { "type": "cds", "sequence": "contig_1", "start": 1002528, "stop": 1003016, "strand": "+", "frame": 3, "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "EC:2.1.1.297", "GO:0006412", "GO:0008757", "GO:0018364", "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4", "UniRef:UniRef90_C4XFW9" ], "nt": "ATGAATAACGAAGAAACATTAATTTTAGAAAAAAAGCGGTATGGTTTGGAACCTAAAATTACTGAAAAAGAATATGAATTATTAAGGCAAAATTATCCAATTCAATTAATAATGGGCTATGTTGAATACTTAAATACTAGAATTAATTTAAGAGAACAAGTTTTAATTCCAAGATATGAAACTGAAGAAATGGTTGATATTTATCTAAAAGAATTTGCAGCTGAAAATCAAGAAGTTTTAGATCTTTGTTGTGGTTCAGGTTTTATAGGTTTAGCAATTAAGAAAAACTTACGTTCAGCTAAAGTAACTTTAGTAGATATTAGTGATGAAGCTATTAAACAAACCATTGAAAATTCTATAGTAAATTTTGGTTTTAATCATGACTTAAAAATTTACCAAAGTGACTTATTTACTAATGTTAAGGGAAGATTTGATGTTATCATTTCAAATCCACCATATTTAAATTGCAATGACAAAAGTTTAGACTGA", "aa": "MNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKEFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLD", "aa_hexdigest": "4d2d5d1b25be6a22fda828c18050ecd3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "prmC" ], "truncated": "3-prime", "pseudogene": { "start": 1002516, "stop": 1003241, "observations": { "mutation": [ 1003014, 1003068, 1003125, 1003164 ], "stop": [ 1003014, 1003068, 1003125, 1003164 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW9", "query_cov": 1.0, "subject_cov": 0.6721991701244814, "identity": 1.0, "score": 321.0, "evalue": 5.56e-110, "gene_start": 1, "gene_end": 162, "reference_start": 5, "reference_end": 166, "reference_sequence": "MAYKMNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKEFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLDWKALSFEPKIALCAPEDGWYFYDKILKKYKNYLKKNGWLIMEINPQHIEKWKQIKGAQIKKDINGKYRFVFIQNI" }, "paralog": false, "identity": 0.979253112033195, "score": 469.0, "evalue": 1.1e-170, "description": "Internal stop codon at 1003014, 1003068, 1003125, 1003164. Nonsense mutation around 1003014, 1003068, 1003125, 1003164.", "psc": { "uniref90_id": "UniRef90_C4XFW9", "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "ec_ids": [ "2.1.1.297" ], "uniref50_id": "UniRef50_A0A3P8KWM4", "go_ids": [ "GO:0006412", "GO:0008757", "GO:0018364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW9", "EC:2.1.1.297", "UniRef:UniRef50_A0A3P8KWM4", "GO:0006412", "GO:0008757", "GO:0018364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KWM4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" } }, "id": "EFPKPENHBP_1837", "locus": "GCA000186005_01830" }, { "type": "cds", "sequence": "contig_1", "start": 1003265, "stop": 1003498, "strand": "+", "frame": 2, "gene": null, "product": "Carrier domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354978.1", "SO:0001217", "UniParc:UPI0001E33019", "UniRef:UniRef100_C4XFW8", "UniRef:UniRef50_A0A3P8MEJ4", "UniRef:UniRef90_C4XFW8" ], "nt": "ATGGAAAAAAATAAAATTAAAGAAATTGTTATTGCAAGATTAGACAAACTTTCAAAGAAAAAGGTAACTTTTACTTCAACACTCGAAGAATTAAAAATTGATTCATTATCACTTGCTGAATTAATTTACGAAAGTGAAATAGAATTCAAAATTAGAATTGAAGATGAAGTGCTTTTAAAAATTAAAAATGTTAATGATATTATTGATGCAATAGAAAATGCTAAGAAAATTTAA", "aa": "MEKNKIKEIVIARLDKLSKKKVTFTSTLEELKIDSLSLAELIYESEIEFKIRIEDEVLLKIKNVNDIIDAIENAKKI", "aa_hexdigest": "612627cfa794abc55c1790cbafd3c523", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33019", "ncbi_nrp_id": "WP_013354978.1", "uniref100_id": "UniRef100_C4XFW8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33019", "RefSeq:WP_013354978.1", "UniRef:UniRef100_C4XFW8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW8", "uniref90_id": "UniRef90_C4XFW8" }, "psc": { "uniref90_id": "UniRef90_C4XFW8", "product": "Carrier domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MEJ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEJ4" ], "product": "Acyl carrier protein" }, "genes": [], "id": "EFPKPENHBP_1838", "locus": "GCA000186005_01831" }, { "type": "tRNA", "sequence": "contig_1", "start": 1003531, "stop": 1003605, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 73.7, "nt": "AGGAGTATAGTTCAATGGTAGAACAACGGGCTTCAACCCCGTGTGTTGTGGGTTCGAGTCCTGCTACTCCTGCCA", "db_xrefs": [], "id": "EFPKPENHBP_14", "locus": "GCA000186005_01832" }, { "type": "cds", "sequence": "contig_1", "start": 1003982, "stop": 1004635, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCATATGGTGTTGAACAAGCTATGTCATCTAAAGCAGACAAACTAGAATTCCTTGAAAAATTAATGTCTGTTGGTGGTGCTAAAATAAAAGAACATGATTCAGCTATTTCCTTTATTAATGCTTATTTATTCAAGATGTTAATTTTCGAAAATGTTAAAGTTTATGAAAGACTTAATCGATTTGACAAAGTAGAATTTACATATGATCAAGTAAATAACAAAGTTGTTGTTAAATTGGTATTTGGATATGAAACACATGTATTTAATATTGATAGCAATAAATTTGACAAGAAAGTTTTAAATGTAATTAAAGTTTTTGCAAACAATGAGTTAAATAAATTTATTGATGAAATTATAAAAAATAATTGCATTAAATTAAAAGATAATAAGTATTATGATGAAAATTTACATGATAGAGATCTTGAAAAAGTATTTGAAATTGATTATACAAAAACGACAGATAAAGGCAACAGATTTAAAGTTAGAATTATTAGTCATAGTTTAAATAATCTTCAAATATCAGTAATAGATACCAAAATGAGAGAAATGTATGGATATAGTTTACCTTATGTAACCGATGGAATTAATTTAGATTTTGATATAGATAAGTTTCTAAGCCCAGTAGCTTCACAACTTAAAGGTATTTGA", "aa": "MSAYGVEQAMSSKADKLEFLEKLMSVGGAKIKEHDSAISFINAYLFKMLIFENVKVYERLNRFDKVEFTYDQVNNKVVVKLVFGYETHVFNIDSNKFDKKVLNVIKVFANNELNKFIDEIIKNNCIKLKDNKYYDENLHDRDLEKVFEIDYTKTTDKGNRFKVRIISHSLNNLQISVIDTKMREMYGYSLPYVTDGINLDFDIDKFLSPVASQLKGI", "aa_hexdigest": "fced11a4ab6d6abd989c03d6f247d26e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25304.79180000002, "isoelectric_point": 6.914542961120605 }, "id": "EFPKPENHBP_1839", "locus": "GCA000186005_01833" }, { "type": "cds", "sequence": "contig_1", "start": 1004783, "stop": 1005295, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAATTAAATTAACACATAATTCAATTAATTCGCCAAAAACCTTTGATCCAAAAATTAATAATAGTTTTCCATTCACAGATTCGAAACTTGAAGAATATGTTAAAACAGACGATTTTAAACACAATTTAGAAGCAAATATTAAAGAAAACTTTTATTTACTTGATTTCATTAAAAAAGAAACTTCTGATAATAAAAAAGATATAAGCAAATTTGTACTTTCTGATTTAGAACTACAAAAAGAATATGATTCAATATTGTTAGATGAAGAATCAAAAAAGATCATTGTTACAAATATTGAAAATTATTTAAATAATAAATTTGATTCTAAATTTAATAAATTTTTAAATTCATATTTCAAACAAGCAGAAATAGCTAGAAAGAACAAAGCGATTAAAGATGATCAAAAATTGTTTAGATATACAATTAAAGAATTCTCAAATATTGATGCCTATCAATCTAAAATTAAAATTCAAGTTGAAATATTCAAAATAGAAGCTAAGGCTTGA", "aa": "MEAIKLTHNSINSPKTFDPKINNSFPFTDSKLEEYVKTDDFKHNLEANIKENFYLLDFIKKETSDNKKDISKFVLSDLELQKEYDSILLDEESKKIIVTNIENYLNNKFDSKFNKFLNSYFKQAEIARKNKAIKDDQKLFRYTIKEFSNIDAYQSKIKIQVEIFKIEAKA", "aa_hexdigest": "1914aaf09749d3d7c07f41e9e9a47405", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.6631, "isoelectric_point": 7.748711967468262 }, "id": "EFPKPENHBP_1840", "locus": "GCA000186005_01834" }, { "type": "cds", "sequence": "contig_1", "start": 1005461, "stop": 1005568, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAGTAATCTTTTTTATACAGTATCAAATAAATTTAAAGCAATAGATTTTACAGATCAAAGTGCTTTAAATGAAATTCAAAGTTATTATGATAATGAATGA", "aa": "MNGSNLFYTVSNKFKAIDFTDQSALNEIQSYYDNE", "aa_hexdigest": "03a5e017bc5a35d1e2d864163aa158e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4068.344, "isoelectric_point": 4.295857048034668 }, "id": "EFPKPENHBP_1841", "locus": "GCA000186005_01835" }, { "type": "cds", "sequence": "contig_1", "start": 1005569, "stop": 1005709, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTTAATAACTTTAAAGATTTAAATCCACTTATTAAATTAGTAGACAGATTGAATAAGTCAATGGTAGCTTATATTAATCCAAATGAAAGTCTTGATACATTTAATGAATCATTAGGACGTCAATTAAAATCTTGA", "aa": "MQFNNFKDLNPLIKLVDRLNKSMVAYINPNESLDTFNESLGRQLKS", "aa_hexdigest": "7abc8d783cdf9c2038af64404423c54f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5357.082399999999, "isoelectric_point": 8.139600944519042 }, "id": "EFPKPENHBP_1842", "locus": "GCA000186005_01836" }, { "type": "cds", "sequence": "contig_1", "start": 1005887, "stop": 1006207, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACGAGCTTTTATATATAATAAAACTAAATTTGTTGTAACTGATTACACAATTAATGGTGATAAAGTAGAATTAAAATTTAAACTTTGTCGTAATTTAGATGATAAAAGTAAAAAGTCATTTTATGAAATATCAACAAAAGAATTCACAAAAGAAATTACTTTAGCTACAGAAAAAACAACAGAAAAAGCATATTTGGACAAACTAATTGATAATTATGACTTTTCACTTCCAGAAGGCTTACAACAATATCATGCCCATAAAGTTCTTTCTCAATTAAAACAAAAACAAATAGGATTTTTTGAACAAAAAAAATGA", "aa": "MERAFIYNKTKFVVTDYTINGDKVELKFKLCRNLDDKSKKSFYEISTKEFTKEITLATEKTTEKAYLDKLIDNYDFSLPEGLQQYHAHKVLSQLKQKQIGFFEQKK", "aa_hexdigest": "17b454cd7f35128e16eb2c479cc310fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12600.306499999988, "isoelectric_point": 9.060338401794432 }, "id": "EFPKPENHBP_1843", "locus": "GCA000186005_01837" }, { "type": "cds", "sequence": "contig_1", "start": 1006298, "stop": 1006450, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCACTACACAAAAAAGACCAACATTTAGAATATCTAAAGAATTAGATGCAATTTTTGACAAAGCAAAAGAGTTTTATCTAAAACCAGAAAACTTCGATATTTACACAGGAGAATTTAAAACACAAGTTTATGGAAAATATAGTTCTTGA", "aa": "MPTTQKRPTFRISKELDAIFDKAKEFYLKPENFDIYTGEFKTQVYGKYSS", "aa_hexdigest": "0a849c2c74d04a35bfc30c682be98b1b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5958.704200000001, "isoelectric_point": 9.003799629211429 }, "id": "EFPKPENHBP_1844", "locus": "GCA000186005_01838" }, { "type": "cds", "sequence": "contig_1", "start": 1006619, "stop": 1006888, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATACTGCAACAGGCGATTTATATATATCGTTTAAATTAAGAGAAAAAGCAAATAATATTTCTGTTGACGGTTATTGTAAAGTTCAAGGTTTCAAACCATTTGATAGTGTTAAAAATCCTGATTTAATAGATGTAGCTAATGCAGCAAAAGCTGTAAATGTTGCGTATGAAGATAAAGGTAGCTTTTCTATTTATGAATATAATGCGAATTTAGTTAAATTTGAAAATTATGATTCATCTATTTATGATTTAAGCCTAGAATCTTGA", "aa": "MDTATGDLYISFKLREKANNISVDGYCKVQGFKPFDSVKNPDLIDVANAAKAVNVAYEDKGSFSIYEYNANLVKFENYDSSIYDLSLES", "aa_hexdigest": "e8eeb3464227932a61b6f2ec77407e02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9949.9192, "isoelectric_point": 4.4735925674438475 }, "id": "EFPKPENHBP_1845", "locus": "GCA000186005_01839" }, { "type": "cds", "sequence": "contig_1", "start": 1007201, "stop": 1007473, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAAAGAAAATTATTCTTAATCCTATCTTCATTAAGTACATTACCTTTTGCTTTTGTAGCAGGTAGTTGTGTTGGTAAAGATAATGGTGGAACAACCGGTAAACCAACTAGTGAAGAAACAAAAGCTGATTTAAATAAACTTGCAAAAAATATTGTTTTTGATGTTAAAAATAAACAAGATAAATTTGCAAAGGATATTAAAAACATATCAAATTTAGTTGTATCAAATTTAGATTTAAATAAATATGAATTAATACATTGA", "aa": "MKNKKRKLFLILSSLSTLPFAFVAGSCVGKDNGGTTGKPTSEETKADLNKLAKNIVFDVKNKQDKFAKDIKNISNLVVSNLDLNKYELIH", "aa_hexdigest": "01c14e5c21a633209d8d8915430d3261", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9955.469499999994, "isoelectric_point": 9.678977012634277 }, "id": "EFPKPENHBP_1846", "locus": "GCA000186005_01840" }, { "type": "cds", "sequence": "contig_1", "start": 1007750, "stop": 1008457, "strand": "+", "frame": 2, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013354980.1", "SO:0001217", "UniParc:UPI0001E3301B", "UniRef:UniRef100_C4XFW6", "UniRef:UniRef50_A0A449B495", "UniRef:UniRef90_C4XFW6" ], "nt": "ATGTCAACACACATCAGCGCAAAAAAAGGTGAAATAGCAAGAATTTGTTTTCTTTCAGGAGACCCTTTAAGATCAAAATTTATGGCTCATAAATATTTAAAAAATGTAAAAGAAGTAAGCTCAGTTAGAAATGAAAATTACTACACAGGAGAATATAGAGGTATCAAAGTTACTTTTGGAGGACATGGAATGGGTATGTTCTCAATTGGAGCTTATGCTCATGAACTTTATAATGAATATGGAGTAGATGTTATTATTCGTTTAGGATCAACTGGTTCATATTATGACGAACACAATGTTATGGATACAGTAATTATTAATAGAGCTTTTAGCGACAACATTTCAATAGCTCAATTAATAAATAAAGAAAATACATATGAATACTTTCCAGACAAAGGTGTAACAGATGAATTAATTAAGGCAGCTAAAAAATTAAACAAAAAAGCACCAGTAGTTTCAGTACACTCAACAGATGTTTTCTATGCAACTAGACCTTTAGAGGAAACTAAGAAAGTATCAAAATGTCAAGTAGTTGAAGCAGAAAGTTATGCGCTTTTTGCAGAAGCTAAAAGAAGCAATAAAAAAGCCGCAACCATTTTACAAGTTAGTGACTCTTTAGCTAAAATGGAATTTAGTGATTATAAAACTAGAGAAACAGGATTTACAGAAATGTTCGAAATAGCTTTGGAAGCATTAAAAAATCTATAA", "aa": "MSTHISAKKGEIARICFLSGDPLRSKFMAHKYLKNVKEVSSVRNENYYTGEYRGIKVTFGGHGMGMFSIGAYAHELYNEYGVDVIIRLGSTGSYYDEHNVMDTVIINRAFSDNISIAQLINKENTYEYFPDKGVTDELIKAAKKLNKKAPVVSVHSTDVFYATRPLEETKKVSKCQVVEAESYALFAEAKRSNKKAATILQVSDSLAKMEFSDYKTRETGFTEMFEIALEALKNL", "aa_hexdigest": "3f221302d8220e1f34074f361ffa8ba9", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3301B", "ncbi_nrp_id": "WP_013354980.1", "uniref100_id": "UniRef100_C4XFW6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3301B", "RefSeq:WP_013354980.1", "UniRef:UniRef100_C4XFW6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW6", "uniref90_id": "UniRef90_C4XFW6" }, "psc": { "uniref90_id": "UniRef90_C4XFW6", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449B495", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_A0A449B495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B495" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "EFPKPENHBP_1847", "locus": "GCA000186005_01841" }, { "type": "cds", "sequence": "contig_1", "start": 1008752, "stop": 1009183, "strand": "-", "frame": 3, "gene": null, "product": "ABC-2 type transporter transmembrane domain-containing protein", "db_xrefs": [ "KEGG:K11051", "SO:0001217", "UniRef:UniRef50_C4XFW5", "UniRef:UniRef90_C4XFW5" ], "nt": "ATGATTTCAACTACAACGACAATTGGTTGTATTACAGTTAGTTTAGGTGCTTCAAGTTTCATGGTTGAAGATATAGAATCAAAAGCCTATAAAGACATTTATGCTTCGCCAGTTAATAAATCTTCAATAATTATTGGAAACGCTATTTCTATTTATGCTGTTACTGTAATTATGTCAACTGTATCATTTATTATTAGTTTTGTGTATGCATTATGCTTTGGAGCAAAATTCTTATCATTTTTACAAATTGTTGAAGTAATTGGATTGATTTTAGTTTCAAGTTTAATAGGAAGTAGTTTTAGCTTTTTAATTGTTTACTTCATTAAAAAACATAATGTTTTTGCAGCTATCAATGCTTTAATTGGATCTATAGCAGGTTTTATAATTGGTGGTTATTCAACAATGGAATCACTGCCTGATGCGCTTCAATGA", "aa": "MISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQ", "aa_hexdigest": "dd84a2f864aefc33be0e99c0a1821b88", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1008503, "stop": 1009396, "observations": { "mutation": [ 1008595, 1008754, 1009186, 1009234 ], "stop": [ 1008595, 1008754, 1009186, 1009234 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW5", "query_cov": 1.0, "subject_cov": 0.48148148148148145, "identity": 1.0, "score": 256.0, "evalue": 5.89e-84, "gene_start": 1, "gene_end": 143, "reference_start": 72, "reference_end": 214, "reference_sequence": "MERRLDNMGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKGWVIEDKSKSAREIMNLWMISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQWIIKCFPASHANVLFRQTLVSPAIDRLISDPTHIEQFKKIMGCFYKFNDYQMQWYVNFIIVAGTSLLLYSIAFGVQFTKKIKN" }, "paralog": false, "identity": 0.9831649831649831, "score": 553.0, "evalue": 1.82e-203, "description": "Internal stop codon at 1008595, 1008754, 1009186, 1009234. Nonsense mutation around 1008595, 1008754, 1009186, 1009234.", "psc": { "uniref90_id": "UniRef90_C4XFW5", "product": "ABC-2 type transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_C4XFW5", "kegg_orthology_id": "K11051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW5", "UniRef:UniRef50_C4XFW5", "KEGG:K11051" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW5" ], "product": "ABC-2 type transporter transmembrane domain-containing protein" } }, "id": "EFPKPENHBP_1848", "locus": "GCA000186005_01842" }, { "type": "cds", "sequence": "contig_1", "start": 1009232, "stop": 1009375, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCAATAATTAAAAGAAATATTAAAAAATATTTCATGGACAAAACTAATGTTTTCTTTTCTTTATTAAGTGTTATTTTAGTCTTCTTTATAGTTGCAGTTTTTTTAGGTAAATCAATTAGTGATGGACTTAAAGGATGA", "aa": "MGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKG", "aa_hexdigest": "047a109a396dc4c8a91c232e14e0c49c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5270.385499999999, "isoelectric_point": 10.121810340881346 }, "id": "EFPKPENHBP_1849", "locus": "GCA000186005_01843" }, { "type": "cds", "sequence": "contig_1", "start": 1009377, "stop": 1009712, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGAAAAAGCTGACTATGTTGTGATTATGGATAATGGAAAAATTGTGGCTAAGAATACTCCTGCAAAACTAAAAGAAGAATATACAAAAGATAGGTTAAAAGTATATCTTGAAAATGATGTAAAGTACTTTAAAACAGTTGAATTGTTAAAAAACAAAGGTTTAAAATTCAAAGAATTTAAAGATTATATTGATATATATTTAGACTCTTCGTTTAAAGCTATAGATTTGGCAAATCAACTTAAAGATTTGATATCTTCTTTCGAAGTTTTAAAGGGTGATATGGATGATGTATTTATCAATATAACTGGAAAGGAGATTAGATAA", "aa": "MEEAEKADYVVIMDNGKIVAKNTPAKLKEEYTKDRLKVYLENDVKYFKTVELLKNKGLKFKEFKDYIDIYLDSSFKAIDLANQLKDLISSFEVLKGDMDDVFINITGKEIR", "aa_hexdigest": "9fb27126814fa2593e4fe5f90b52dca4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12938.804299999985, "isoelectric_point": 5.269167900085448 }, "id": "EFPKPENHBP_1850", "locus": "GCA000186005_01844" }, { "type": "cds", "sequence": "contig_1", "start": 1009773, "stop": 1010297, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "nt": "ATGTCTGAAATAATAAAAGTTGAAAATTTAAAAAAATCATATGGCAAAGTTGAAGCTGTCAAAGGTATTTCTTTCATTGTAAAAGAAGGAGAGCTTTTTGCATTTTTAGGGCCTAATGGTGCAGGCAAAAGTACCACAATTGATACCTTGTGTACATTGAATGATTATAATGAAGGCCAAATAACAATTTGTGGTTTTGATTTAAAGAAACAAAGTGATGAAATCAAAAAACAAATTGGTGTGGTTTTTCAAGATAGTGTATTAGATCGAAAATTAAGTGTTAGAGATAATTTAACAATTAGAGTAGCATATTATTATCGTAAAAAAGCAGATATAAAAGCAGCTTTTGAAAGGGTTGTAAAAGATACTCAAATTGAAGATATTTTGAAAAGAAAATATGAAAAACTTTCAGGTGGTCAAAGACGTCGTGTGGATATAGCTAGAGCCTTATTAAATTATCCAAAAATTTTATTTTTAGATGAACCAACAACAGGATTAGATCCTCAAACTAGAAAGCTTATTTGA", "aa": "MSEIIKVENLKKSYGKVEAVKGISFIVKEGELFAFLGPNGAGKSTTIDTLCTLNDYNEGQITICGFDLKKQSDEIKKQIGVVFQDSVLDRKLSVRDNLTIRVAYYYRKKADIKAAFERVVKDTQIEDILKRKYEKLSGGQRRRVDIARALLNYPKILFLDEPTTGLDPQTRKLI", "aa_hexdigest": "072bbd7f5a004fc9b37f656454934128", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002079168B", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.626, "score": 223.0, "evalue": 3.47e-71, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI002079168B" }, "pscc": { "uniref50_id": "UniRef50_UPI002079168B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "EFPKPENHBP_1851", "locus": "GCA000186005_01845" }, { "type": "cds", "sequence": "contig_1", "start": 1010477, "stop": 1012291, "strand": "+", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "RefSeq:WP_013527161.1", "SO:0001217", "UniParc:UPI0001F5992C", "UniRef:UniRef100_C4XFW3", "UniRef:UniRef50_A0A6M4J8T6", "UniRef:UniRef90_C4XFW3" ], "nt": "ATGGCATATAAAGCACTTTATCGAAAATATCGTCCGAAGTCTTTTGATGATGTTAAGGGTCAAGATCATATTGTGCAAACATTAAAAAATATCATTTTAAATCACAAGATAAGTCATGCATACTTATTTAGTGGACCAAGAGGTGTTGGTAAAACTTCAGTTGCTAAAATTTTTGCTAATGTAGTTAACTGTGGTCATGGTGAAGATTTAACTAAACTTTGTGAAAGTTGTATTGAAAAAGCGGATCAAAATTTTGATGTTATCGAAATGGATGCTGCTTCAAATAATGGTGTTAATGAAATTAGAGATTTAAGAGATAAAATCCAACATTCACCAGCAAGTGGAAACTACAAAGTTTATATAATCGATGAAGTTCACATGCTTTCAAAAGGAGCTTTTAATGCACTTTTAAAAACATTAGAAGAACCACCAGCACATGCAATATTTATTTTAGCAACAACTGATCCTCAAAAAATACCTCTAACAATTCTTTCAAGAGTCCAAAGATATAATTTTAGGAAAATATCAAACCAAGTTCTAATTAACCAAATTAAAGAGGTCTTAGATAAAGAAAAAATTAAATATGAAGATTCAACCATAGCTTATATTGCAAGATTAGCAACTGGTGGTATGCGTGATGCACTTTCAATAGTCGACCAAGCTGCAGCTTATGGTAACGACAAAATATCGCTCAAAGATGTTATGTATGCTTTTGGAATTACTTCAAATGAAAGCTTAATTCAAATTGTAAACTACCTTTATCAAGGCAAAATCAAGGAAGTCTTAATTTTATTTAATAACCTTAAAAATGCGGGAATTGATTCAAATCAATTTGTTCAAAGCTTAATAGAATTGTTCAAGGATTACTTGATTTATGCAAAATCAAATGATGCTAATTTATTAGAACTTTTATCTCTTGAAGAATTAAATACTTTGAAAATTGATGTTAATTTTGCATTCAATTCATCTGAAAAACTTTATAAATTATTCAAAGAATTAATTTTTTCAGAAAACCCATTCCAATTAATCGAACTTTATTTAATTAAACTTAATCAAACTAATGAATTACAAAATAGTAAGGAGCAAACTATGTCAATTGATGTAAACAAAAAGAAACCTAAAAAAGATGACACAATCAAAACAGTTTTAAATCAAACTCAAGAATTAGTACTTGAAGCTAATCGTGACGCTGATGACAATTTTAGCCTAGAACAAGATCTTCTCAGTAGCAATTTTGAAATAGAAGAAGATTATGATGATGGTTTAGTAAGTACAGCAGAAATTCAACTAGATGATGAAATTCAAAATATGCCAAAACCAGTAGTAATCCCTGCTTTTGACTCAAAATACAAAAACTTGAAAACATTCAAATCAAACTTAAGTAAAAAAGATTTAATCAACTTACTTAAACAATCAACATTAACATTTTTAAATAGATACAAATCAAATTTAGATATTGTGATTAACACAGTTCAAAATCCAATTTACAGTGATTTAATTACAGTACTTAAAAAATGTAAAATTGCCGCAGCAGGCCCTAATTACATGATTTTTATCTCAAAAAATGTACCAGAATTAGTTTATTTACAAGAAAATGGTTACAACTCAAATATTCAAAAATTTATTCAAGATTACTTTGAAGATTACAAACACTTATACTGCATTATGAGTGATGATTACAAAGAAACAGCAAAAGATTTTGCAGCTAAAATGAAAGATGCAAAATTTGACACAAGCTTTACAGTACCAGGACCAGTTGTTTTACAAAATGTAAAGAGTCCAACAGGCAAATTATTTGATAAATTCAAAAAATAA", "aa": "MAYKALYRKYRPKSFDDVKGQDHIVQTLKNIILNHKISHAYLFSGPRGVGKTSVAKIFANVVNCGHGEDLTKLCESCIEKADQNFDVIEMDAASNNGVNEIRDLRDKIQHSPASGNYKVYIIDEVHMLSKGAFNALLKTLEEPPAHAIFILATTDPQKIPLTILSRVQRYNFRKISNQVLINQIKEVLDKEKIKYEDSTIAYIARLATGGMRDALSIVDQAAAYGNDKISLKDVMYAFGITSNESLIQIVNYLYQGKIKEVLILFNNLKNAGIDSNQFVQSLIELFKDYLIYAKSNDANLLELLSLEELNTLKIDVNFAFNSSEKLYKLFKELIFSENPFQLIELYLIKLNQTNELQNSKEQTMSIDVNKKKPKKDDTIKTVLNQTQELVLEANRDADDNFSLEQDLLSSNFEIEEDYDDGLVSTAEIQLDDEIQNMPKPVVIPAFDSKYKNLKTFKSNLSKKDLINLLKQSTLTFLNRYKSNLDIVINTVQNPIYSDLITVLKKCKIAAAGPNYMIFISKNVPELVYLQENGYNSNIQKFIQDYFEDYKHLYCIMSDDYKETAKDFAAKMKDAKFDTSFTVPGPVVLQNVKSPTGKLFDKFKK", "aa_hexdigest": "7a64fe6ac682e2a25e81fcfbfc18e131", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F5992C", "ncbi_nrp_id": "WP_013527161.1", "uniref100_id": "UniRef100_C4XFW3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5992C", "RefSeq:WP_013527161.1", "UniRef:UniRef100_C4XFW3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW3", "uniref90_id": "UniRef90_C4XFW3" }, "psc": { "uniref90_id": "UniRef90_C4XFW3", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A6M4J8T6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4J8T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4J8T6" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "EFPKPENHBP_1852", "locus": "GCA000186005_01846" }, { "type": "cds", "sequence": "contig_1", "start": 1012339, "stop": 1012635, "strand": "+", "frame": 1, "gene": null, "product": "Nucleoid-associated protein MBIO_0769", "db_xrefs": [ "KEGG:K09747", "RefSeq:WP_013354985.1", "SO:0001217", "UniParc:UPI0001E33020", "UniRef:UniRef100_C4XFW2", "UniRef:UniRef50_C4XFW2", "UniRef:UniRef90_C4XFW2" ], "nt": "ATGGATCAAGCAATGTTAAGAAAGATGCAAAAATTGCAACAAGAAGTTCAAGAAAAAACTGAACAATTCATGGAAGAAACATTTAAATTAGAAAAACATGGAATTGAAGTTACAGCTAAAGGAAGCAAAAAAATAGTTTCAGTAAAAATCAAAGATTCTATTCTTTTAGACCCAGATGATGCTGAAACTTTAGAAGACTTGTTATCACTTGTGATTAATGAGTTATTTGACACAATTGATGAAGAACAAGAAAAATTAATGCCAGCTATGCCTGGTTTAGGTGGACTTGGCTTCTAA", "aa": "MDQAMLRKMQKLQQEVQEKTEQFMEETFKLEKHGIEVTAKGSKKIVSVKIKDSILLDPDDAETLEDLLSLVINELFDTIDEEQEKLMPAMPGLGGLGF", "aa_hexdigest": "ab897048bca2ffa3e26156e29058872f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33020", "ncbi_nrp_id": "WP_013354985.1", "uniref100_id": "UniRef100_C4XFW2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33020", "RefSeq:WP_013354985.1", "UniRef:UniRef100_C4XFW2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW2", "uniref90_id": "UniRef90_C4XFW2" }, "psc": { "uniref90_id": "UniRef90_C4XFW2", "product": "Nucleoid-associated protein MBIO_0769", "uniref50_id": "UniRef50_C4XFW2", "kegg_orthology_id": "K09747" }, "pscc": { "uniref50_id": "UniRef50_C4XFW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW2" ], "product": "Nucleoid-associated protein MBIO_0769" }, "genes": [], "id": "EFPKPENHBP_1853", "locus": "GCA000186005_01847" }, { "type": "cds", "sequence": "contig_1", "start": 1012638, "stop": 1013228, "strand": "+", "frame": 3, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "KEGG:K06187", "RefSeq:WP_013354986.1", "SO:0001217", "UniParc:UPI0001A8C12E", "UniRef:UniRef100_C4XFW1", "UniRef:UniRef50_N9VD56", "UniRef:UniRef90_C4XFW1" ], "nt": "ATGAAAATTGAAACAATTGAAAAACTTAATCAAGTTTTAACAACAATTCCAGGAATTAGTAAAAAGCAAGCAGACAAAATGTCAAACTTTTTCTTAAAACAAGATCAAGAATATATTGACAATTTAGTTAAGTCAATTAATGATCTTAAAACCAACATTTCATATTGTTCTATTTGTAACTTTTTAAAAGAAGGTGGCAAATGTTTAGTATGTGATGATAAGAGCAGATACAACTTTTTAATGATAGTTGAAAATGCTGCAACTTTAAGAAAAATTGATGACATGAATTTTTACAATGGCTACTACTATGTTTTGCCTTATTTGTTTAATTTAAAGAATAATAAGGAAATTAAAAACTATGATTACAAAGAATTAATAAACTATATTCAACATAAAAAATTTGATGAAGTAATCATAGTTTTGAGCCCTTCTTTAGAAGGCGAAATGACAACAGGTCATTTATTAAAATTGATTAATAGCCAATTTAAAATTAAAGTAACAAGAGCAGCTATTGGCATGCCTATGGGTTCAAATTTAGAATATCTTGATACTTTCACAATCAAACAATCTATTGAAAATAGAAACAAATAA", "aa": "MKIETIEKLNQVLTTIPGISKKQADKMSNFFLKQDQEYIDNLVKSINDLKTNISYCSICNFLKEGGKCLVCDDKSRYNFLMIVENAATLRKIDDMNFYNGYYYVLPYLFNLKNNKEIKNYDYKELINYIQHKKFDEVIIVLSPSLEGEMTTGHLLKLINSQFKIKVTRAAIGMPMGSNLEYLDTFTIKQSIENRNK", "aa_hexdigest": "723c7d4573e29f587df86bd8be7458b4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12E", "ncbi_nrp_id": "WP_013354986.1", "uniref100_id": "UniRef100_C4XFW1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12E", "RefSeq:WP_013354986.1", "UniRef:UniRef100_C4XFW1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW1", "uniref90_id": "UniRef90_C4XFW1" }, "psc": { "uniref90_id": "UniRef90_C4XFW1", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_N9VD56", "kegg_orthology_id": "K06187" }, "pscc": { "uniref50_id": "UniRef50_N9VD56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VD56" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "EFPKPENHBP_1854", "locus": "GCA000186005_01848" }, { "type": "cds", "sequence": "contig_1", "start": 1013248, "stop": 1013502, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02223.23" ], "nt": "ATGTTTATTACTTTTGAAGGACCAGATGGTAGTGGTAAAACTACAATAATTAACCGCCTTATTATTGAATTAATTGAAAAATATCCAAAGCTTGCATTAAATTATGTTTTGACTCGTGAACCGGGTGGCAAAGACATAATTGAAGCAGAAAAAATTAGATCATTAATTTTGGATAAAGATTCAAAGCTTTCTTCAGTTTCTGAAGCTCTTTTATATACAACTAGTCGTCGAATTCATCTTGAAAGAGTAGTATGA", "aa": "MFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVV", "aa_hexdigest": "00831f06906d099994faeb4c3b971296", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02223.23", "name": "Thymidylate_kin", "length": 79, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9113924050632911, "evalue": 2.5476679766592148e-14, "score": 49.67196273803711, "start": 5, "stop": 83 } ], "seq_stats": { "molecular_weight": 9517.996399999996, "isoelectric_point": 8.0078275680542 }, "id": "EFPKPENHBP_1855", "locus": "GCA000186005_01849" }, { "type": "cds", "sequence": "contig_1", "start": 1013530, "stop": 1013886, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_Q4A6Z8", "UniRef:UniRef90_C4XFW0" ], "nt": "TTGTGCGATCGCTATGTCGACAGCTTTTTTGCATATCAAGGTTATGCTCGTAATTTAGGTATTACTTTTGTTAAAAAACTTACTAATTTAATAATTGATAAAACTATGCCTGATATTACAATCTTTTTTGAAATCAAACCTGAAGAAAGTAAAATCAGAAGAGAACAAATAAGAATTGTTAGTGACAGATTAGACAGTGAAACCGAATTATTCCACCAAAAAGTTTACAAAGGTTATCATGAGCTAATTAAAAAAGAAAAAGATAGATTCATTGTTGTTGATGCTACAAAAAGTATTGAAGAAGTTTTAAAAGAATCATTTGAAAAATTAGATAAACATCCAAGATTTAAAAAATGA", "aa": "MCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKK", "aa_hexdigest": "59c1993f650aa9d68c50d1277b0c8e06", "start_type": "TTG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "both", "pseudogene": { "start": 1013242, "stop": 1013904, "observations": { "mutation": [ 1013500, 1013884 ], "stop": [ 1013500, 1013884 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW0", "query_cov": 1.0, "subject_cov": 0.5363636363636364, "identity": 0.992, "score": 228.0, "evalue": 3.12e-74, "gene_start": 1, "gene_end": 118, "reference_start": 97, "reference_end": 214, "reference_sequence": "MIMFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVVWPALKENRLVLCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKKWIKEFK" }, "paralog": false, "identity": 0.9863636363636363, "score": 422.0, "evalue": 5.06e-153, "description": "Internal stop codon at 1013500, 1013884. Nonsense mutation around 1013500, 1013884.", "psc": { "uniref90_id": "UniRef90_C4XFW0", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_Q4A6Z8", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW0", "EC:2.7.4.9", "UniRef:UniRef50_Q4A6Z8", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6Z8" ], "product": "Thymidylate kinase" } }, "id": "EFPKPENHBP_1856", "locus": "GCA000186005_01850" }, { "type": "cds", "sequence": "contig_1", "start": 1013904, "stop": 1014422, "strand": "+", "frame": 3, "gene": null, "product": "DNA polymerase III subunit delta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9", "UniRef:UniRef90_C4XFV9" ], "nt": "ATGGTTTCAAAAAATGTCATAACTATTTTAGAAAACTCAATAAAAAATAACAAACTTAGTCACTGTTATTTACTAAAAGCTCCTTCAGGTTTAAACATCGATGATTCAATTTTATACATGATTAATAAACTTTGCAACTCACAATTGCAATCTTTAGATCTTGAAACAATGCTGCCTAATGTTCAAATTTTTGAAGATAATGGCACTGAAAATAATCTTAAAAAAGATAAAATTGTGGCCGGCTTTGAAGAATCAAGTTTATCTTCATTTGTTGAAGGGCAAAAGAAGATAACTGTATTTAAAAATGTTGAAAATGCTTCTAAAGCTGCTCTTAATTCATTACTTAAAATAATTGAAGAACCAAGTGATAATGTAGTCTTTATTTTAACAACTAATAACATAACAAAAGTTTTAGAAACCATCAAATCAAGATCAATAACAATTAACATTAATGCGCCTTCAAACGAAGAGTTAGCAGAAGAATTAAAAGGTGAAAATTTAAAAGAAGAAGCTATATGA", "aa": "MVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAI", "aa_hexdigest": "b8b186c364a1adcf83121e6898721d43", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1013901, "stop": 1014725, "observations": { "mutation": [ 1014420, 1014630, 1014636 ], "stop": [ 1014420, 1014630, 1014636 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFV9", "query_cov": 1.0, "subject_cov": 0.5620915032679739, "identity": 1.0, "score": 318.0, "evalue": 1.26e-107, "gene_start": 1, "gene_end": 172, "reference_start": 2, "reference_end": 173, "reference_sequence": "MMVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAIWFYSHIFADLTQVKKYTNNESFKLIKDLLEAIEKSIKNPHYLYIFLSRFTKKDSKDTFVFLAMALRFVFSWVWTADAFCRKNYAKLLVKLKNIKFDLFNCFIVIDDFLKIQNTNANFFLQAEKMLIKLVESYA" }, "paralog": false, "identity": 0.9854014598540146, "score": 520.0, "evalue": 9.84e-190, "description": "Internal stop codon at 1014420, 1014630, 1014636. Nonsense mutation around 1014420, 1014630, 1014636.", "psc": { "uniref90_id": "UniRef90_C4XFV9", "product": "DNA polymerase III subunit delta", "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFV9", "UniRef:UniRef50_C4XFV9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9" ], "product": "DNA polymerase III subunit delta" } }, "id": "EFPKPENHBP_1857", "locus": "GCA000186005_01851" }, { "type": "cds", "sequence": "contig_1", "start": 1014814, "stop": 1015338, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal RNA small subunit methyltransferase I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "nt": "ATGCCTAAAATATATATTGTTGGAACACCTATTGGAAACTTAAAAGATATAACTTTAAGAGCTCTTGAAACTCTTAAGGCTGTTGATGTTATTGCTTGCGAAGATACTCGTGTTAGTGCTAAATTATTGAACCATTATGACATTAAAAAACGTTTAATTTCATACAACAAAATCAATGAAAAAGCAAGTGCCCAAGGCCTTATTGATTTAATTGAAAAAGATGACTTAAAAATGGCTTTAATTAGTGATGCAGGTATGCCTTTAGTTAGTGATCCGGGTTTTGAATTAATTAAACAAGCAAGAGAAAAAAATATTGAATTAGAACTAATTCCAGGAGTCAATGCAGCTATTAGTGCTTTTGCTTTTAGTGGACTAAGTAATACCTTTATTTTTATGACTTTTCCTAAAGAAAAAAGTGGCCAAAGATTAGAACAAGTCAAAAGCTTCACAAGTGAACATGCATATGTTTTTTATGTAGCTCCGCATAAATTAGAAAGTTTCTTAAAAGACATTGACTCAGTGTGA", "aa": "MPKIYIVGTPIGNLKDITLRALETLKAVDVIACEDTRVSAKLLNHYDIKKRLISYNKINEKASAQGLIDLIEKDDLKMALISDAGMPLVSDPGFELIKQAREKNIELELIPGVNAAISAFAFSGLSNTFIFMTFPKEKSGQRLEQVKSFTSEHAYVFYVAPHKLESFLKDIDSV", "aa_hexdigest": "620f13f2f9abb04a43e6141dc9760c69", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0010050DA8", "query_cov": 1.0344827586206897, "subject_cov": 0.8181818181818182, "identity": 0.5, "score": 168.0, "evalue": 9.39e-50, "valid": false, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [], "id": "EFPKPENHBP_1858", "locus": "GCA000186005_01852" }, { "type": "cds", "sequence": "contig_1", "start": 1015360, "stop": 1015524, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAGAGCTTACTAAAATACATGAAGAATTTATTTATGCAACCGCAACTGAACTATTAGCAAAAAACAAAACTAGTCCTTTTAAAGGTGAGATAACTTTAGTTATCAAACTAAAAGAAATTAAAAAAGAGAAAATAAATAAATATGCTCACTTAAAATAA", "aa": "MARELTKIHEEFIYATATELLAKNKTSPFKGEITLVIKLKEIKKEKINKYAHLK", "aa_hexdigest": "11e25ee0c2d353e13d5e9c128a343d0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6301.4431, "isoelectric_point": 9.647580909729005 }, "id": "EFPKPENHBP_1859", "locus": "GCA000186005_01853" }, { "type": "cds", "sequence": "contig_1", "start": 1015603, "stop": 1015842, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ], "nt": "ATGAATCATATTAATATTTTTGCTCTTGGTGGGCTTGATGAAAATGGTAAAAACTGTTATGTTTTCGAATTCAATGATGACATTTACATTATTAACTCAGGAACTAAAATTCCAATCAACTCAAATAATGGAGTTGATACTTTAATTCCTTCTTTTGAATACTTAGAAAAACACAAAGATAGAATTAAAGGTATCTTTATTTCTGATGTTAAAAATGAATCGTTTAGTGCATTGCCTTGA", "aa": "MNHINIFALGGLDENGKNCYVFEFNDDIYIINSGTKIPINSNNGVDTLIPSFEYLEKHKDRIKGIFISDVKNESFSALP", "aa_hexdigest": "cf88b56f74275f1fbf1d11bd63f36936", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002201F3D4", "query_cov": 0.9746835443037974, "subject_cov": 0.875, "identity": 0.636, "score": 108.0, "evalue": 1.61e-29, "valid": false, "uniref50_id": "UniRef50_UPI002201F3D4" }, "pscc": { "uniref50_id": "UniRef50_UPI002201F3D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8909.930499999995, "isoelectric_point": 4.8840837478637695 }, "id": "EFPKPENHBP_1860", "locus": "GCA000186005_01854" }, { "type": "cds", "sequence": "contig_1", "start": 1015849, "stop": 1017255, "strand": "+", "frame": 1, "gene": null, "product": "Putative hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84", "UniRef:UniRef90_C4XFV7" ], "nt": "ATGAAAATTCCTAACTTAACAATTTATACTTCAGCATTCAACAAAATCATGATCATGGATCGTTTATCAAAATATAAGATTCCAACAAAAAACTACAAAGTTATGGTTATTAATAAAGTTACACCATTTAGTGACAAACTTTTTGTTAAGCCAATCGATTTAGCTGGTTCAATGCCAGGACACATTGGTTTTGACTTCATCACACCAGATGGAGATATCTTATTTATGTTTAACTTTGTCGAAGGTGACTTAGGAATTTATGGTAAATTAAGCTTTAATGAACTTAAACAAAGATTTACAAAACGTAAAATTTTAGCTCTAGTTGTTGACTCAGGTAAAGCAAACTTTGGTGGTAAGGCTATTGAAAAAATTGGTTTACCAGAAGGTATTAGAGATGTTTTTTTAGAAACCAAAAAGAATGAAAGAATAATAGTTGGTGCTTATGACGAAGAAATGGTAGCTATCCACAAAATTTTAGAATTAGCTCAAGAAACAAGTCGTCCAGTTGTAACTTATGGTAAAACTTATGGCCAAATATTTTATTTAATTAAAAAAGCTCATCCTGAACTTAAGTTACCAGAATTAATTGATTATCGTAATGCTAACAAAGTTAAAAATGCTGTTATTTTAGTAACTGGTGCTACTGAAAGACTTTACTCAAGATTTATTAGAATAACTGACAATAATGATGTTTTTTTAAAACTTCAAAAAAGCGATACTGTTGTAATGATTGCTCCTCCTATTAATGGACTTGAAAGTCTTGAAGCTTTAACTTTAGATGAAATTGCAAGAATTACTCCAAAAATTAGTGATGTTAATGCTAATGAATTTTATAGATGTCGTCCTGCTAAACAAGACTTAATTGATTTAGTTAATGCTCTAAATCCTGAATATGTGATTCCTGTGCAAGGACTTTATCGTTACTTAAATGAAGCTACTATAGCTATAAGTGAAAATACACAAGTGAAACAAAATCATTGTTTAGTTATGCAAAATGGAAAAATTGTTCACTTTATTGATGGCAAAATGTCAACAACCAAGGGCAAAGTTAAAGAAATTGGCGACACTATTATTGATGGTTTTGGTGTTGGCGATATTAGTACTGAGGTTATTAGTGAGCGTGAAGCTTTAGGTCGTGAAGGTGTGATTTTAGTGTCTTCTCAATATAATCCAAAAACAAAATTATTAACTGGTAAATTACAAATAAATTTTGTTGGTGTCATAAACAAAGAGGAAAAAAATGAAGCTAGCGACATAATCAAAAGCACAATTGTAAAATTAATTGAAACAGAAAGTTTTAATGGAATAAAAGATTTCCAAAATGAAGCTCGTCATGCTATTAGAAAAAGAATTTACAAAACTTTTGAAAAAGAACCAATAGTAATTGTAACATTGGCTCAAATTTAA", "aa": "MKIPNLTIYTSAFNKIMIMDRLSKYKIPTKNYKVMVINKVTPFSDKLFVKPIDLAGSMPGHIGFDFITPDGDILFMFNFVEGDLGIYGKLSFNELKQRFTKRKILALVVDSGKANFGGKAIEKIGLPEGIRDVFLETKKNERIIVGAYDEEMVAIHKILELAQETSRPVVTYGKTYGQIFYLIKKAHPELKLPELIDYRNANKVKNAVILVTGATERLYSRFIRITDNNDVFLKLQKSDTVVMIAPPINGLESLEALTLDEIARITPKISDVNANEFYRCRPAKQDLIDLVNALNPEYVIPVQGLYRYLNEATIAISENTQVKQNHCLVMQNGKIVHFIDGKMSTTKGKVKEIGDTIIDGFGVGDISTEVISEREALGREGVILVSSQYNPKTKLLTGKLQINFVGVINKEEKNEASDIIKSTIVKLIETESFNGIKDFQNEARHAIRKRIYKTFEKEPIVIVTLAQI", "aa_hexdigest": "b3d877f488c310ec82246759f5e90521", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFV7", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 1.0, "score": 915.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_A0A448ZZ84" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84" ], "product": "Putative hydrolase" }, "genes": [], "id": "EFPKPENHBP_1861", "locus": "GCA000186005_01855" }, { "type": "cds", "sequence": "contig_1", "start": 1017789, "stop": 1018151, "strand": "+", "frame": 3, "gene": null, "product": "Monocytic differentiation/activation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932", "UniRef:UniRef90_P94932" ], "nt": "ATGAAAAAGTCAAAAAAAATTTTATTAGGATTGAGTCCTATTGCTGCTGTTCTTCCTGCAGTAGCTGTTTCTTGTGGAAACAACGATGAATCCAATATTTCATTCAAAGAGAAAGATATTAGTAAATATACCACAACAAATGCTAATGGAAAACAAGTTGTTAAAAACGCTGAATTGTTAAAATTGAAACCAGTTCTTATTACAGATGAAGGTAAAATTGATGATAAATCATTTAACCAATCAGCTTTTGAAGCTTTAAAAGCTATAAATAAACAAACTGGTATTGAAATTAACAGTGTTGAACCTAGCTCAAACTTTGAAAGTGCTTACAACAGTGCACTTTCAGCCGGACACAAAATTTGA", "aa": "MKKSKKILLGLSPIAAVLPAVAVSCGNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPSSNFESAYNSALSAGHKI", "aa_hexdigest": "ed58f4c0702204caebd15e4aa8c69aeb", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1017789, "stop": 1018347, "observations": { "insertion": [ 1018131 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P94932", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 0.95, "score": 213.0, "evalue": 6.56e-69, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MKKSKKILLGLSPIAAVLPAVAVSCGNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPISNFESAYNSALQPDTKFEYLMASNTNNLLNNTLMLTEKNLKEIKSKSLVSTLILKQSTSDSTHYNSILKNLHLQQAMQLQVD" }, "paralog": false, "identity": 0.9945945945945946, "score": 347.0, "evalue": 3.67e-124, "description": "Frameshift due to insertion around 1018131.", "psc": { "uniref90_id": "UniRef90_P94932", "product": "Monocytic differentiation/activation factor", "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P94932", "UniRef:UniRef50_P94932" ] }, "pscc": { "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932" ], "product": "Monocytic differentiation/activation factor" } }, "id": "EFPKPENHBP_1862", "locus": "GCA000186005_01856" }, { "type": "cds", "sequence": "contig_1", "start": 1018530, "stop": 1018832, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484", "UniRef:UniRef90_UPI00217FF484" ], "nt": "ATGAACACTGTTATTAATAATGTTTTATCTTCAACACCAGCTGATGTTAAATACAACCCACATGTTATCTTATCTGTTGCTGGACCTGCTACATTTGAAACTGTAAGATTAGCAAACAAAGGTCAATATGTAATTGGTGTTGACTCAGACCAAGGTATGATTCAAGACAAAGACAGAATTCTTACATCAGTTCTAAAACACATTAAACAAGCTGTTTATGAAACATTATTAGATCTTATTCTTGAAAAAGAAGAAGGATATAAACCATATGTAGTTAAAGACAAAAAAGCAGACAAAAAATGA", "aa": "MNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKK", "aa_hexdigest": "ae68c9deea6e7b6e28f31007589be251", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1018332, "stop": 1019075, "observations": { "mutation": [ 1018341, 1018830, 1018860 ], "stop": [ 1018341, 1018830, 1018860 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217FF484", "query_cov": 1.01, "subject_cov": 0.40725806451612906, "identity": 0.99, "score": 190.0, "evalue": 2.76e-59, "gene_start": 1, "gene_end": 100, "reference_start": 67, "reference_end": 167, "reference_sequence": "MASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLAANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKKWSHFGTQKEKWIGVAENHFSNTEEQAKINNKIKEAIKMFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK" }, "paralog": false, "identity": 0.9758064516129032, "score": 466.0, "evalue": 2.12e-170, "description": "Internal stop codon at 1018341, 1018830, 1018860. Nonsense mutation around 1018341, 1018830, 1018860.", "psc": { "uniref90_id": "UniRef90_UPI00217FF484", "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217FF484", "UniRef:UniRef50_UPI00217FF484" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11159.751899999994, "isoelectric_point": 7.90738582611084 }, "id": "EFPKPENHBP_1863", "locus": "GCA000186005_01857" }, { "type": "cds", "sequence": "contig_1", "start": 1018941, "stop": 1019075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAGAATTACCAGAAGATTTCGTTAAATATATTAATAGTGACAAAGCTTTAAAAGATGGTAATAAAATTGACAATGTTAGTGAAAGATTAGAAGCAATTATTTCTGCTATTAACAAGGCAGCAAAATAA", "aa": "MFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK", "aa_hexdigest": "e7960324786cd46f128a8fa539f7add7", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4967.6506, "isoelectric_point": 6.125163078308105 }, "id": "EFPKPENHBP_1864", "locus": "GCA000186005_01858" }, { "type": "cds", "sequence": "contig_1", "start": 1019221, "stop": 1019571, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTAAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFKPL", "aa_hexdigest": "a34e56f8fcbc4f5181b851514f9999e3", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13575.125699999975, "isoelectric_point": 10.064046669006348 }, "id": "EFPKPENHBP_1865", "locus": "GCA000186005_01859" }, { "type": "cds", "sequence": "contig_1", "start": 1021525, "stop": 1022628, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "nt": "ATGAACGCAATTGAATTTATTAATATTTCGAAAAGTTTCGGTGTAATCAAAGCTAACCAAGATATTAATATTCAAATTAAAAAAGGTACAATTCATGCACTTATTGGTGAAAATGGTGCAGGTAAAAGTACCTTGCTATCAATTCTTTTTGGACTATATGAACCCGACCATGGAATTATCAAGGTTAATGGTAAAGAGGTTTTAATAAATAATCCAAATGATGCTAATAATTTGGGTATTGGTATGGTTCACCAACATTTTAAATTGGTTGGTGCATACACAAACTTACAAAATATAATTCTTGGTGCTGAAAGTGAATCAAAAACTTTTAAAACTTTGGATTATAAACCATCAATTAAAAAAATTAAAACTATTCAAAGATTATTTAATTTAAATTTTGATTTGAATAAAGACACAGGCACTGAATCAGTTTCAACACAGCAAAAAGTTGAAATCATGAAGATGTTATATAGTGATTCAGAAATTCTAATCTTTGATGAACCAACTGCTGTTTTAACAGATGAAGAAATTCAAGGTCTTCTTGAAACATTCAAATTGTTTAAATCGCAAGGTAAAACAATCATTTTTATCTCTCACAAACTAAAAGAAGTTAAAGCTGTAGCTGATTATGCAACAGTTATACGTCACGGTAAAGTTACTGGTAACTTCGAAGTTGCTAATACTTCTATTGAAGAAATGGCTAACAGAATGGTTGGTGACAATATTGTTATGGCACAAAACCAATCACTTGACGTTTTTGAAGATAAAGACACAGTTTTAGAACTTGAAAATGTATCAACAAAAGGTACAAAATCACTTAAAAACTTAAATCTTAAGGTTCATGCTGGAGAAATTCTAGCTATTGCTGGTGTTGAAGGCAATGGACAACAAGATTTAGAATATGTTGTTAGTGGAATTGTTAAACCTACAAAAGGTAATGTCTACTTAAAGAAAACACCTTTAGTTGAGGAAAGAGTTAAATTCATTCATAAAAATAAAATCAAAAATTTCATTATTTTAAGTTCATTGCTTGCATTATTTATAGCTTTTGGATCAATTTTTTTTGGTGTTAGTCCATTTCAGCAAAATTATTCAAACATTTGA", "aa": "MNAIEFINISKSFGVIKANQDINIQIKKGTIHALIGENGAGKSTLLSILFGLYEPDHGIIKVNGKEVLINNPNDANNLGIGMVHQHFKLVGAYTNLQNIILGAESESKTFKTLDYKPSIKKIKTIQRLFNLNFDLNKDTGTESVSTQQKVEIMKMLYSDSEILIFDEPTAVLTDEEIQGLLETFKLFKSQGKTIIFISHKLKEVKAVADYATVIRHGKVTGNFEVANTSIEEMANRMVGDNIVMAQNQSLDVFEDKDTVLELENVSTKGTKSLKNLNLKVHAGEILAIAGVEGNGQQDLEYVVSGIVKPTKGNVYLKKTPLVEERVKFIHKNKIKNFIILSSLLALFIAFGSIFFGVSPFQQNYSNI", "aa_hexdigest": "d5424ca12f9532327e1233767766894c", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00111B63FE", "query_cov": 0.9700272479564033, "subject_cov": 0.9246753246753247, "identity": 0.64, "score": 435.0, "evalue": 2.43e-149, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A223M8X7" }, "pscc": { "uniref50_id": "UniRef50_A0A223M8X7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "EFPKPENHBP_1866", "locus": "GCA000186005_01860" }, { "type": "cds", "sequence": "contig_1", "start": 1022686, "stop": 1022880, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAAAAATTATTAAATTCTTTAAAACCAAAAAAGATGAAGAGAAACTTAAAGACAGGATTAATTTAAACAATTATTCAGTCTTAGAAAAATCAATGCTTGCTTCAAGTTTTATTCCAAGTGATAGACATAAACATGGTCTAGCATTAGATTACAATATTAAAAATAATACAATATTAAGACGTTTATGA", "aa": "MIVKIIKFFKTKKDEEKLKDRINLNNYSVLEKSMLASSFIPSDRHKHGLALDYNIKNNTILRRL", "aa_hexdigest": "a620a6519f26e53850423a242e50ae21", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7590.869299999998, "isoelectric_point": 9.994485282897951 }, "id": "EFPKPENHBP_1867", "locus": "GCA000186005_01861" }, { "type": "cds", "sequence": "contig_1", "start": 1023043, "stop": 1023327, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCACCACACGACTTTATATTAATTCTTCAACCAACACGTGGACTTGACGTTGGGGCTATTAAAAATATCCATGAAAGAATTCTTAAAGAAAAAGCAGAAGGAAAAGCAATTCTTTTAATTTCTTATGAATTAGACGAGGTTCTTTCACTAGCTGATACTATTGCTGTTATTAACGAAGGCGAAATTAGACTTTCAAAACCCGCAAGAGAATTAACTAGAAAAGAAATCGGTCTTTACATGGCTCAAAAAGAACATCAAACGGAAGGAGGCACACAATAA", "aa": "MNSPHDFILILQPTRGLDVGAIKNIHERILKEKAEGKAILLISYELDEVLSLADTIAVINEGEIRLSKPARELTRKEIGLYMAQKEHQTEGGTQ", "aa_hexdigest": "0f4e1c1824615bf988e90214beb30225", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10521.004699999992, "isoelectric_point": 5.577576255798339 }, "id": "EFPKPENHBP_1868", "locus": "GCA000186005_01862" }, { "type": "cds", "sequence": "contig_1", "start": 1023557, "stop": 1023667, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATAATCCAACTTTAAAACCGATTGAAACAGCTAAACCAGTAAAACCAAATATTACAAAAAATGTTGCAAAGTCAAATTTAATGTTTTTGTCAATAAAGCTATAA", "aa": "MLNNPTLKPIETAKPVKPNITKNVAKSNLMFLSIKL", "aa_hexdigest": "31116f07fdd34202f464adf72bacbc7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4007.8476, "isoelectric_point": 10.301161384582517 }, "id": "EFPKPENHBP_1869", "locus": "GCA000186005_01863" }, { "type": "cds", "sequence": "contig_1", "start": 1023918, "stop": 1024220, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATCCAGAATCATATTCACCTACAACTATAGGTAGTGCTAGAATTGCAATTGAAAATGCAGTTAAATATCAATTTGTTTACTTTGGTATTGGAATGTTTGTTTTAACAGCTATTGTTTTATTTGTGATCTACAACTTCACAACATTAGGTTACAAAATAAACATGGTTGGTAAAAATCCTTCAAACGCTAAATATATTGGTCTTAACGAAAAAATGTTAACCATTATAGTTATGGGATTTTCAGGAGCTTTAGCTGGTTTCGCTGGATTCTACTACATTATTATCAAAGAAGGCTGA", "aa": "MFNPESYSPTTIGSARIAIENAVKYQFVYFGIGMFVLTAIVLFVIYNFTTLGYKINMVGKNPSNAKYIGLNEKMLTIIVMGFSGALAGFAGFYYIIIKEG", "aa_hexdigest": "118af7f5e609274ee04915dd610c8ecf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11045.922399999992, "isoelectric_point": 9.222927665710447 }, "id": "EFPKPENHBP_1870", "locus": "GCA000186005_01864" }, { "type": "cds", "sequence": "contig_1", "start": 1024335, "stop": 1024475, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACAACAAGAGATTTATTTGCTATAAAAGATAAATTAACAAAAGACTTTTTCCCTATTATTACTGGTATAATTGTCTTTATGGCTGCTTTAGCTATTATGCTTTATAAATTCAAACCACTTGGTTTCATGTGA", "aa": "MIYTTRDLFAIKDKLTKDFFPIITGIIVFMAALAIMLYKFKPLGFM", "aa_hexdigest": "94e0e6c73b4c18eca007b21badbcf0dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5348.580299999999, "isoelectric_point": 9.601614952087402 }, "id": "EFPKPENHBP_1871", "locus": "GCA000186005_01865" }, { "type": "cds", "sequence": "contig_1", "start": 1024530, "stop": 1024646, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCAAAAATGCTAAATATAGAGAATATGTTAAAGCTCTTAATGAAGAAAAAAGAAGGAATAGAAAACTCAAAAAAGAATTTGCTTCAATTAAAGAAGAATATCAAAAATGA", "aa": "MKTKNAKYREYVKALNEEKRRNRKLKKEFASIKEEYQK", "aa_hexdigest": "a34dee78871f7ca0cfabb5913749dc8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4777.5102000000015, "isoelectric_point": 10.060952186584473 }, "id": "EFPKPENHBP_1872", "locus": "GCA000186005_01866" }, { "type": "cds", "sequence": "contig_1", "start": 1024695, "stop": 1024862, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATCTATGACGAAATGTCTAAGAAAAAATTCGAATATCTCAAAAAATACAGTGATTTTGGCTTAAACAATCTTAAAACATTAAAAAATCAATATAAAGCTGAAAAACACGAAAGAAAAATTCAATATATTACTAAAAGAGATTTAATTTATACATCTCATTGA", "aa": "MSIYDEMSKKKFEYLKKYSDFGLNNLKTLKNQYKAEKHERKIQYITKRDLIYTSH", "aa_hexdigest": "f2d3fd8a02e7960c808e320debbeaf2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6808.792300000001, "isoelectric_point": 9.619150352478027 }, "id": "EFPKPENHBP_1873", "locus": "GCA000186005_01867" }, { "type": "cds", "sequence": "contig_1", "start": 1024976, "stop": 1025143, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGCTCTAACACTTGCAACCCTTTCAGGTATTTTCTCAGAAAGAGTTGGTATTACAAACATTGCTGTTAACGGGTTTATGATTTTTGGAGCTGCTATGTATGCTCTAATAAGCTTCCTAATGACTGATGTTGTTTTCAAAGGTAAAACATTAAGTCCATGA", "aa": "MFSALTLATLSGIFSERVGITNIAVNGFMIFGAAMYALISFLMTDVVFKGKTLSP", "aa_hexdigest": "e07f8485bfe935da0a934d138da5976c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5864.954900000001, "isoelectric_point": 8.253774452209473 }, "id": "EFPKPENHBP_1874", "locus": "GCA000186005_01868" }, { "type": "cds", "sequence": "contig_1", "start": 1025153, "stop": 1025383, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACTTTGCTTTTGCAGGTATCTTATCCTCGGGATTTGCACTGTTGTTTGGTTTCGCAACGATTAAACTTAAATCAGATCAATCTGTTTCAGGTTTCGCTATGAACATTCTTGCAGTTGGTGTTGCTTCTATCCTTTTATTATTTGTTAAAAAAGCACAACAAGGTGGAGACTACTTCCAAATGCGTGGCCAAACAGAACTTGCTTTAAGTCCTAATGTTGACAACTGA", "aa": "MYFAFAGILSSGFALLFGFATIKLKSDQSVSGFAMNILAVGVASILLLFVKKAQQGGDYFQMRGQTELALSPNVDN", "aa_hexdigest": "71a4c945dcbd207a07abe44b623c494e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8126.381999999997, "isoelectric_point": 8.098147773742678 }, "id": "EFPKPENHBP_1875", "locus": "GCA000186005_01869" }, { "type": "cds", "sequence": "contig_1", "start": 1025777, "stop": 1025917, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTGTTTCAAGATTCGCTGAACTACTCTACACATTGCCATTTATTATCACACTTGGAATTATGATTGCTAAAATGAAAAGCAAGCAAATGGGGCCAGCTGCTGCAGGTCGACCTTATGACAAATCAGAGCGTTAG", "aa": "MKGVSRFAELLYTLPFIITLGIMIAKMKSKQMGPAAAGRPYDKSER", "aa_hexdigest": "dd7652daf9c1160972968470b421fec8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5117.128300000002, "isoelectric_point": 10.11536350250244 }, "id": "EFPKPENHBP_1876", "locus": "GCA000186005_01870" }, { "type": "cds", "sequence": "contig_1", "start": 1025936, "stop": 1027978, "strand": "+", "frame": 2, "gene": null, "product": "Phase variable surface lipoprotein P78", "db_xrefs": [ "RefSeq:WP_013527169.1", "SO:0001217", "UniParc:UPI00000B5B26", "UniRef:UniRef100_O06764", "UniRef:UniRef50_O06764", "UniRef:UniRef90_O06764" ], "nt": "ATGAAAAAAAGAAAATTTATATTTGCTTTATCATCATTAACAACAGCTTTTTTAGCTACACCTCTTATTTCTTCATCATGTACTAATGCTGATAAATTGTCTAATCTATTAGATGATTTAGACATTACTATTCCAGATTCAGAATCTAAAAAACCAAATGAAATCAAAGAATCGGATTTAAAATTTTACTCAAAAAATATGCCAAGTTTTAATAGTGAATATACTGTTGAAATACAAAAAATTCAAAAATATTCTTCAGAACTTGTAGTAACTTTTAGACTACGCGAAAAAAACTCAAACATTGTTTCAAAATACAAAACAATTACATACAAAAATTTCTTAAACTTTGATCATATTAATGAAGCTTTAACAGTACAATATTCTAATTCAAAAAATACTTATGTAAAAGATGTTGCTCAAACAAGTGACATTATCTTTAGAGATATTAAAACAGGATACAAAATTATCGATGTTAAAATTGATAACTCAAGAATTGAAGAATATCAAAAAAATTTATCTGACAAACTAAAAGATATAAATGAATTCTTAACAAAAAATAAATCTGAATATGATGCTGAAAAAGCTAAAGCAAATCCTAATAAGGAAATTATTGAAAAATATGAACGCGCTTTAATTAGAAAAGCTAAATATGAAGATACTGCGGATAAAGATTCATATATCAAATCATTCGAGAATTTAAATATAAAATACAAAATTCAATCAATAAATGATCCTAAAATTGTATCTGATGAATTCAGTCGTTCAATTCCAAACTTCAAAAATGATTCATACATTGATTACTATGTTTTTGATAAAAGTGACACATACTCAACAGTTTCGCATTCATTTCACTCTGTTTCAGATTTAACTGAAGAAATAAAGAAATACATTAAAAAAACTGCTTCACTAGAATATACAGGAAATACAAATGTTTCTGTATCTGAAGTTAAGGCTGATAAATTTAAATTTAATTTTGACACACCAATTGATTTTTCAATAGAAAACATCAAAGTTGAACCTGCTGATTCAGAAGGCAAAGTAAATCTTAAATTTAATATTAAAGCACCTATTAACAAACAAGCAACAACTTTATTAGTTGATGAAAACAAAAAAACAATTGAATTTGATTATGCATGTGAAATAGAATTTAACAAAAAAGATAGTAAAGAAAAATTAAGTGAAATTGAAAAACAAATTAGCTTTACATATGAAAATGCTGCAACAACAGAAGTTGATAAGGCAGAAGTTTCTAAAGTTAAATACACTTTATCTAACCCAAATTATGAAATTACTGATTTAAAAATTATTGGTTATGAATTCAACAATTCAACTATTGTTATTGAATACAAAGCAAAAAGTAAAAGCAGTTCAATTGAACAAGTTTATTCAAGAACAATTAGTGGCTTTTCTAAACTTTCACAATCTAAAATTGATGCAATTTTAGATTCAATTAAATTGAATTATAAACGTAAAAACTTAGTAGCTGCAAATGAAGCGAATAAAGATCATGTATTTATTGAATCAATGGATTCAAACTTCATGATTGAAGTTACAAAAATAGATTCATTTGATCTTAAAGATAAATCAATATTAGTTGAATTTAAATTATATGAAGTTGATGCACTTTCTAAATTAAAACCAGACAACAAAAAACGCGGTTTCAAATTTGTTAAATCATTCACAAAGAAAATTACAGGCTTCTCAGAAAAATCATTGACACAACATATCAATGAAGAAATTCAAAAGGTTACTTGTACTTGTCCAACAGCTTCAAGCAAATTGCCAAGTCAATTAAACATTAATGATATTAAATTTACTAACTTTGATAACAATATTTTTGAAATTGAAAAACCATCAATAATTGTTGCTAATGATCAAAAAGGCGAAGTTGTTATTACATTTAAATTACGTTACAAAATTGGTTATGTACAAAAAGTTTATGACCAACAAAAGAATGAATATGTTGAAAAAGAAATACCTTTCAAAACTGTTTCAGACTCAAAAACAGTTATATTAAATGGTTTTAAAAAAAATAAATTATAA", "aa": "MKKRKFIFALSSLTTAFLATPLISSSCTNADKLSNLLDDLDITIPDSESKKPNEIKESDLKFYSKNMPSFNSEYTVEIQKIQKYSSELVVTFRLREKNSNIVSKYKTITYKNFLNFDHINEALTVQYSNSKNTYVKDVAQTSDIIFRDIKTGYKIIDVKIDNSRIEEYQKNLSDKLKDINEFLTKNKSEYDAEKAKANPNKEIIEKYERALIRKAKYEDTADKDSYIKSFENLNIKYKIQSINDPKIVSDEFSRSIPNFKNDSYIDYYVFDKSDTYSTVSHSFHSVSDLTEEIKKYIKKTASLEYTGNTNVSVSEVKADKFKFNFDTPIDFSIENIKVEPADSEGKVNLKFNIKAPINKQATTLLVDENKKTIEFDYACEIEFNKKDSKEKLSEIEKQISFTYENAATTEVDKAEVSKVKYTLSNPNYEITDLKIIGYEFNNSTIVIEYKAKSKSSSIEQVYSRTISGFSKLSQSKIDAILDSIKLNYKRKNLVAANEANKDHVFIESMDSNFMIEVTKIDSFDLKDKSILVEFKLYEVDALSKLKPDNKKRGFKFVKSFTKKITGFSEKSLTQHINEEIQKVTCTCPTASSKLPSQLNINDIKFTNFDNNIFEIEKPSIIVANDQKGEVVITFKLRYKIGYVQKVYDQQKNEYVEKEIPFKTVSDSKTVILNGFKKNKL", "aa_hexdigest": "a5904c6bb81d6f611ee07803c8fe3e62", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000B5B26", "ncbi_nrp_id": "WP_013527169.1", "uniref100_id": "UniRef100_O06764", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B5B26", "RefSeq:WP_013527169.1", "UniRef:UniRef100_O06764" ] }, "ips": { "uniref100_id": "UniRef100_O06764", "uniref90_id": "UniRef90_O06764" }, "psc": { "uniref90_id": "UniRef90_O06764", "product": "Phase variable surface lipoprotein P78", "uniref50_id": "UniRef50_O06764" }, "pscc": { "uniref50_id": "UniRef50_O06764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O06764" ], "product": "Phase variable surface lipoprotein P78" }, "genes": [], "id": "EFPKPENHBP_1877", "locus": "GCA000186005_01871" }, { "type": "cds", "sequence": "contig_1", "start": 1028282, "stop": 1028371, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGAAATTAATCAAGATGAATACAAGAACAATGTTATTGGAAAAGAAAAAGGCATTTATGTTTTGGTTTTTCACGCATTATGA", "aa": "MLKEINQDEYKNNVIGKEKGIYVLVFHAL", "aa_hexdigest": "fbeb342cc3e90903ea2cbb81877de32e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3406.9455000000007, "isoelectric_point": 6.523149681091309 }, "id": "EFPKPENHBP_1878", "locus": "GCA000186005_01872" }, { "type": "cds", "sequence": "contig_1", "start": 1028387, "stop": 1028506, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCTTCTTTAGAACAATTAGACAAATTAGACAACATTCCAGTTTATCGTGTAGATGTTGATGAAAATACAAAGCTAACTCAAGAAATGGGTGTTAATTCAATGCCAACATGA", "aa": "MFKSSLEQLDKLDNIPVYRVDVDENTKLTQEMGVNSMPT", "aa_hexdigest": "444ad36c6f5862ce8e726c07b9f6df7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4487.048000000001, "isoelectric_point": 4.385492134094237 }, "id": "EFPKPENHBP_1879", "locus": "GCA000186005_01873" }, { "type": "cds", "sequence": "contig_1", "start": 1028833, "stop": 1029087, "strand": "+", "frame": 1, "gene": "ssrA", "product": "SsrA RNA (TmRNA)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "nt": "ATGTTAGTTAAGTGTGATGAAACTAGAGATCGCAAATTATTGATGCATAAAAATGAAATAGTTAGATTGCAGTCTAAACTTCATAAGCTAGGTCATGCAACTATTATTCCAAGCAAAATTTATTTTGATAATCGCTCACGTATTAAGATTGAAATAGCTTTAGTTCAAGGAATGAAAAAAACAGATAAACGTGAAGAAATCAAAAAAAGAGATAATGAAAGATACATCAAAAAAGTCTTGAAAAATGTATATTAA", "aa": "MLVKCDETRDRKLLMHKNEIVRLQSKLHKLGHATIIPSKIYFDNRSRIKIEIALVQGMKKTDKREEIKKRDNERYIKKVLKNVY", "aa_hexdigest": "7f4e784c6ff2976e5520b178335a04d8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P825", "query_cov": 0.9642857142857143, "subject_cov": 0.8901098901098901, "identity": 0.506, "score": 81.3, "evalue": 1.95e-18, "valid": false, "product": "SsrA RNA (TmRNA)-binding protein", "uniref50_id": "UniRef50_A0A3B0P825", "kegg_orthology_id": "K03664" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "product": "SsrA RNA (TmRNA)-binding protein" }, "genes": [ "ssrA" ], "id": "EFPKPENHBP_1880", "locus": "GCA000186005_01874" }, { "type": "cds", "sequence": "contig_1", "start": 1029605, "stop": 1029724, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAGTTTTCTTTCTTCCTGAAGATCCTCAAAACACAATTACAAACTATATTAAGAACACAAATTTTGAATCAAATATTTTAGTTTATGGAAAAGAAGTAGAAACATTAAATTGA", "aa": "MAIVFFLPEDPQNTITNYIKNTNFESNILVYGKEVETLN", "aa_hexdigest": "22f8da0404af7745050b8ab6046c161f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4521.060600000001, "isoelectric_point": 4.251295280456543 }, "id": "EFPKPENHBP_1881", "locus": "GCA000186005_01875" }, { "type": "cds", "sequence": "contig_1", "start": 1029731, "stop": 1029841, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGAATTGAACAAGGAGCAAAGACAAGAAATAGAAAAGTTGTTATAACTCACTCTTCACCTAATCCTGAAGAATATATTGAAACTATCAAATATGTTTCAAGCTGA", "aa": "MNGIEQGAKTRNRKVVITHSSPNPEEYIETIKYVSS", "aa_hexdigest": "cf9bdc96af1bf43440c5738923bdd480", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4077.5335000000014, "isoelectric_point": 8.161713600158691 }, "id": "EFPKPENHBP_1882", "locus": "GCA000186005_01876" }, { "type": "cds", "sequence": "contig_1", "start": 1029866, "stop": 1030072, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00356.27" ], "nt": "ATGAAAAATTTCTCATATAAAAATATTGCTAAAGAAGCAGGTGTCTCTATTAGTACTGTAAGCCGTTACTATCGTGGTGGTTATGTTAGCAAGGAAACTAAAAAAGTTATTGCTAATATCATAAAAGAAAACGATTATTATCCAAATCATGGAGCCAGAACAATTCGTGGCCGTGACACTTCTATCTTTATTATTGTGCCTGAGTGA", "aa": "MKNFSYKNIAKEAGVSISTVSRYYRGGYVSKETKKVIANIIKENDYYPNHGARTIRGRDTSIFIIVPE", "aa_hexdigest": "ec7b210ac875650da08381d424306d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00356.27", "name": "LacI", "length": 44, "aa_cov": 0.6323529411764706, "hmm_cov": 1.0, "evalue": 5.242608966666621e-15, "score": 51.34090042114258, "start": 7, "stop": 49 } ], "seq_stats": { "molecular_weight": 7759.767100000002, "isoelectric_point": 9.791796684265137 }, "id": "EFPKPENHBP_1883", "locus": "GCA000186005_01877" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 1030232, "stop": 1030328, "strand": "+", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 46.4, "evalue": 1.4e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "TTGTAGCGTCAGTAGAACAATAACCTTGTAACCTGGTCAGGTCAGAGATGAAGCAGCCATATCGAGTAGTGTTGTGTCTATTGACTTTTTTTATTTT", "id": "EFPKPENHBP_44", "locus": "GCA000186005_01878" }, { "type": "tRNA", "sequence": "contig_1", "start": 1030527, "stop": 1030600, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 66.5, "nt": "GCGAATGTAATTTAATGGTAAAATGCCAGCTTGCCATGCTGATAATGGGGGTTCGATTCCCCTCGTTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 1030551, 1030553 ], "id": "EFPKPENHBP_15", "locus": "GCA000186005_01879" }, { "type": "cds", "sequence": "contig_1", "start": 1031107, "stop": 1031811, "strand": "-", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "nt": "ATGGCTGTTAACAAAGTGTTTTCAGATTCATTTGAACGTTTCTTAAATGAAAATCCTGCTGAAGCAAAAACTATTGTTCAAAAAACAATTTTTGCTAAAAAATCTCGTCTTGCTGGTTTAGCAGCTAGAGATGCAGCTAGAAGAAAAACTGTTTTTGATACAGGTTCACTTCCAGGAAAATTAGCTGACTGTTCTTCAAAAAATGCTGAAATTAGTGAGCTTTACATTGTCGAAGGTAACTCAGCTGGTGGTTCAGCTAAAATGGGAAGAAACAGAATGACTCAAGCTATCTTGCCTCTTCGTGGTAAAGTTATTAATGCTGAAAAGAATCAACCTAAAAAAGTGTTTGCAAATGCTGAAATTCTTTCATTAATTACAGCACTTGGAACAGGTGTCGGTGAAGAATTTAATATTAATAAATTAAGATATCACAAAATTATCATCATGACCGATGCCGATGTCGATGGTGCTCACATTAGAACATTGTTATTGACGTTCTTCTACCGTTACTTTAGACCTTTAATTGAATACGGATTTATTTATATTGCTCAACCTCCTTTATATAAAATAGCTCAAAACAAATTTATTGGATATGCTTACAACGATGCTCAAAAAGAAGAAATTATGCAAGGCTTAAATCAAAATCAAAAAGTAACAGTTCAACGTTACAAAGGACTTGGTGAAATGGACCCTGAACAACTTTGA", "aa": "MAVNKVFSDSFERFLNENPAEAKTIVQKTIFAKKSRLAGLAARDAARRKTVFDTGSLPGKLADCSSKNAEISELYIVEGNSAGGSAKMGRNRMTQAILPLRGKVINAEKNQPKKVFANAEILSLITALGTGVGEEFNINKLRYHKIIIMTDADVDGAHIRTLLLTFFYRYFRPLIEYGFIYIAQPPLYKIAQNKFIGYAYNDAQKEEIMQGLNQNQKVTVQRYKGLGEMDPEQL", "aa_hexdigest": "b0dd183ac76f8b59f866f16a6b0b59f4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A380DNC1", "query_cov": 0.9914529914529915, "subject_cov": 0.8083623693379791, "identity": 0.586, "score": 276.0, "evalue": 1.96e-90, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A380DNC1" }, "pscc": { "uniref50_id": "UniRef50_A0A380DNC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "EFPKPENHBP_1884", "locus": "GCA000186005_01880" }, { "type": "cds", "sequence": "contig_1", "start": 1032472, "stop": 1032717, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGGCAGGTTTTGCAAACAAAGTAGAAATCACGATTACAAAGGATGGTTACATTGATGTTATCGATAATGGTCGTGGTATTCCTACAGCAGTTCACCCTAAAACTGGTCTTTCAACAGTTGAAACAGTTTTAACGGTTTTACATGCTGGCGGTAAGTTTGACTCTGACTCATATAAAGTTTCTGGTGGACTTCACGGGGTTGGTGCTTCAGTAGTTAATGGACTTAGCGATGACTTTGAAGTTTGA", "aa": "MAGFANKVEITITKDGYIDVIDNGRGIPTAVHPKTGLSTVETVLTVLHAGGKFDSDSYKVSGGLHGVGASVVNGLSDDFEV", "aa_hexdigest": "cdea0663282a34fd64db0bcb153c438c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 1.0, "subject_cov": 0.84375, "identity": 0.58, "score": 102.0, "evalue": 6e-27, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 77, "aa_cov": 0.9135802469135802, "hmm_cov": 0.8181818181818182, "evalue": 1.704822929520493e-11, "score": 40.88747787475586, "start": 4, "stop": 77 } ], "seq_stats": { "molecular_weight": 8347.249399999999, "isoelectric_point": 5.032149314880371 }, "id": "EFPKPENHBP_1885", "locus": "GCA000186005_01881" }, { "type": "cds", "sequence": "contig_1", "start": 1032748, "stop": 1032867, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAAGAATATGATGCATCGAATATTAAAGTTTTAGAAGGATTAGAAGCCGTTAGAGTTAGACCTGGTATGTACATCGGTACAACAGGAATTAGAGGTCTTCACCACCTTATTTGA", "aa": "MSKEYDASNIKVLEGLEAVRVRPGMYIGTTGIRGLHHLI", "aa_hexdigest": "a62aa19c0147e26c6a8c113928cd8d31", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4326.009699999999, "isoelectric_point": 8.230243492126466 }, "id": "EFPKPENHBP_1886", "locus": "GCA000186005_01882" }, { "type": "cds", "sequence": "contig_1", "start": 1033204, "stop": 1033506, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAATAAATCACAAAATGATGAAAAATTAAATCAATCATCAGTCGAAGAACATAATGTAAATGAAGAACATACACAAAAACAAAAAGACAAAGAAGTGATGGACGAGGTTAATGCATTCGAAAGAGACGTTTTACTTAATAATGGTAGAGTCGAAGACAAGCATGTTGAAGAACATCATGAAATGAATTCAATTCATTTAGATGACGTTGTTAACAAAAAAAATACTCAATACAAATACACAAAAATGTCGAATTTTGTGTTGAAATTATCTCGTTTTTATGCTCCTATGCCTCTTTGA", "aa": "MPNKSQNDEKLNQSSVEEHNVNEEHTQKQKDKEVMDEVNAFERDVLLNNGRVEDKHVEEHHEMNSIHLDDVVNKKNTQYKYTKMSNFVLKLSRFYAPMPL", "aa_hexdigest": "add831090532a0044360f9a2be48465e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11884.080499999991, "isoelectric_point": 5.637370872497558 }, "id": "EFPKPENHBP_1887", "locus": "GCA000186005_01883" }, { "type": "cds", "sequence": "contig_1", "start": 1034020, "stop": 1034244, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTTTCTTCTTTGCAATTATTGCAATAATAGGAGGTAGTGCAGGAGTTACTGGAATTATTGGCGAATATATGGCTGTCGTTAAACAAAAAAACTTTGGAACAATTAATGGTTATATCAACTTAGTAATTATTATTGTTTCAGTTGTACTTGGTACATACTTACCAGGAAGTTTATTATTAAATGACATTAATGGTTTTAAAGCAGATTCAGTGACTTGA", "aa": "MLAFFFAIIAIIGGSAGVTGIIGEYMAVVKQKNFGTINGYINLVIIIVSVVLGTYLPGSLLLNDINGFKADSVT", "aa_hexdigest": "8e8453df4bfebabf6b9316a0efcba96b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7738.106599999996, "isoelectric_point": 5.875696754455566 }, "id": "EFPKPENHBP_1888", "locus": "GCA000186005_01884" }, { "type": "cds", "sequence": "contig_1", "start": 1034368, "stop": 1034670, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTACCTTAACAAATTATTCCCTCGTTACAAAATTGTTCAAGTTAAAATTTACTCGCCTCACATGAGTGCTATTAGAGAAGCAATTATTAGTGATAATAAAACTGTTAATAGCTTTACTATCACTAAAGGCGTTGGAGGTTACTCAGGAAATAACACCAAAGTTCTTTCATCAATTACTCTTTACAATCAAGTGCCACGTCTTATTAAGAAAGTTCGGGCCGTTGATCAAAACTCGCTGATTACTATTAGTAATGTTGCTAGTGTTGATGGAAAAATATATCTTCCTGAAAACAAATTCTAA", "aa": "MYLNKLFPRYKIVQVKIYSPHMSAIREAIISDNKTVNSFTITKGVGGYSGNNTKVLSSITLYNQVPRLIKKVRAVDQNSLITISNVASVDGKIYLPENKF", "aa_hexdigest": "b27be12c95e6d88241986559dd2e78de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 53, "aa_cov": 0.53, "hmm_cov": 1.0, "evalue": 7.88063577025533e-14, "score": 47.618621826171875, "start": 39, "stop": 91 } ], "seq_stats": { "molecular_weight": 11222.946299999989, "isoelectric_point": 9.99583911895752 }, "id": "EFPKPENHBP_1889", "locus": "GCA000186005_01885" }, { "type": "cds", "sequence": "contig_1", "start": 1034737, "stop": 1034844, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAACTATATTGAATACAATGGCGACAAAGTTCCTTTATATATAGAAGATAACAAAAAAAATACAACTTTTTTGTTTCTTCATGGACTTAATTCAAACTGA", "aa": "MKLNYIEYNGDKVPLYIEDNKKNTTFLFLHGLNSN", "aa_hexdigest": "2a8e932a89320bde0eb10530101fc8ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4147.662500000001, "isoelectric_point": 6.517181587219238 }, "id": "EFPKPENHBP_1890", "locus": "GCA000186005_01886" }, { "type": "cds", "sequence": "contig_1", "start": 1034880, "stop": 1035014, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCACTTTTTTAGTTTTAATTCTATTTAAAACCTCTTTAGCTACTTCAATTCATCACTCAAGTTTAATTTCTTCTGGTTCTACATTTTTGAAAAATTTACTTCCTGGAAAATTAATAGCCACAACATTGTAA", "aa": "MTTFLVLILFKTSLATSIHHSSLISSGSTFLKNLLPGKLIATTL", "aa_hexdigest": "07f256863019d40f7c953417b06d93ed", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4717.610199999998, "isoelectric_point": 10.301419258117672 }, "id": "EFPKPENHBP_1891", "locus": "GCA000186005_01887" }, { "type": "cds", "sequence": "contig_1", "start": 1035028, "stop": 1035168, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGGTGGCGTGGCTACTGTTTTAGCTAAGGATCCTAAAGTTGTTAGATTAATTATGATGTCAACAATTAATCCTTATATGAAAGAATCTACTTCTTATTCAGTGTTACAATCTGTTATTAAACCTCAAAATAAATGA", "aa": "MAGGVATVLAKDPKVVRLIMMSTINPYMKESTSYSVLQSVIKPQNK", "aa_hexdigest": "9eb0c225c598cdb542dc8fee2cfafe2d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5025.969600000002, "isoelectric_point": 9.873542594909669 }, "id": "EFPKPENHBP_1892", "locus": "GCA000186005_01888" }, { "type": "cds", "sequence": "contig_1", "start": 1035301, "stop": 1035525, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAATTAGATTTACAATACAAAGCAAATTGTCACAAAATGCTCTTTTTAGTAGGTGAAAAGGACAAAATTATTGGTACTAAATATTTTGAAGATTATGCAATTAGTTTAGAAGTTCCAATTTTAATTATGGGTACGGGACACTCGCCAATTAAAGATGAACCAAAAAGAATTGCAAGATTTTTTAATGCATTGATTGAAAGCAAAAAAAGACACTTTTGA", "aa": "MEQLDLQYKANCHKMLFLVGEKDKIIGTKYFEDYAISLEVPILIMGTGHSPIKDEPKRIARFFNALIESKKRHF", "aa_hexdigest": "be5977e579ddc5690897837d38251f35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8656.085799999993, "isoelectric_point": 8.761269569396973 }, "id": "EFPKPENHBP_1893", "locus": "GCA000186005_01889" }, { "type": "cds", "sequence": "contig_1", "start": 1035535, "stop": 1035627, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAAAATGAATCAATTCTTAGAATTTGATGAAGAAAAAACAAAAGAAGAAGATATTCTTCTTGAACTAGAACAAAACGTTGATTAG", "aa": "MNIKMNQFLEFDEEKTKEEDILLELEQNVD", "aa_hexdigest": "a270d6dae8cbc8a6f02061a52e16896c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3686.078700000001, "isoelectric_point": 4.09805736541748 }, "id": "EFPKPENHBP_1894", "locus": "GCA000186005_01890" }, { "type": "cds", "sequence": "contig_1", "start": 1035637, "stop": 1036128, "strand": "+", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XFU3" ], "nt": "ATGGAATACAATCAATTTGAAATGGAACTTAGAAGTGCTTTAACAACAGGCAATGGTACAAAACTTTTATACAAAATAATTGATGCACCTTATAGATTTTTTTCACCTTTGCAACCTTTTAATTACAAAGTTAAATTAGAACAAGCATTCTTAAGAACACAAGAAAACACATATGTAAAATTTATTAAATATTGTGCTGAATATTTAATTACTCAATATGGTTTTAAATTAGAAAAAAGTTCTTTTAAATATACTTTACCACTTAAAGAGGGAGAAGAATTCCCAGAAGAAGTAAAAGTCAATGCAGCTATGATTTTTAGACAAAAAGAAAATGGCGGAGATGAAGAAACAGCTGATATTTGTGTAATTTACTTGAAAAAAAGAGATTACTATGGAGCTAAAGAAGTTACTAAACTTCATGACAATATCATTAAGCAATTAGAAGTATTAAGAATAATTTATCCTAATAATAAAATCAAAGGTATCTTATGA", "aa": "MEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGIL", "aa_hexdigest": "811897c888de565fa2d70d057c393c28", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1035631, "stop": 1036431, "observations": { "mutation": [ 1036126, 1036264, 1036390 ], "stop": [ 1036126, 1036264, 1036390 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU3", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 1.0, "score": 323.0, "evalue": 1.25e-109, "gene_start": 1, "gene_end": 163, "reference_start": 3, "reference_end": 165, "reference_sequence": "MIMEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGILWFLDNDYHKNVNFFNECYDKEHSFETPGFRAYYGAQFFKIFDKEEDWLLVENHIKKFKNSNYDYFLKMPDLDQDEDVLNAMINLNETSWQKLISPDEVYANIRQYIFNERNENSNLFKALKLRDIKRTSVDDDEEKRRILELENKANQNE" }, "paralog": false, "identity": 0.9849624060150376, "score": 533.0, "evalue": 6.43e-195, "description": "Internal stop codon at 1036126, 1036264, 1036390. Nonsense mutation around 1036126, 1036264, 1036390.", "psc": { "uniref90_id": "UniRef90_C4XFU3", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU3", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "EFPKPENHBP_1895", "locus": "GCA000186005_01891" }, { "type": "cds", "sequence": "contig_1", "start": 1036655, "stop": 1036909, "strand": "-", "frame": 3, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "nt": "TTGCCTTCAGAAATTAAGTATCGTGAAATATCATCAGTTTCATATATGGGAGATTTCCAAGCACGTCGTGCAATGATTAGATATAAGAATGAAAATGGTGAAAATGTTTATGCTCATACCATGAATGGTTCAGGTCTTGCAATTGATAGATTAATTGCAGCAATTCTCGAAAATTATCAAAACAAAGATGGTTCAATTACTATACCTAAGAAATTAGTTCCTTATATGGACGGAATAACAAAAATTACAAAATAA", "aa": "MPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK", "aa_hexdigest": "5f51a468b16bf6f300185d105ab8bf5c", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI00387B1930", "query_cov": 0.9404761904761905, "subject_cov": 1.0, "identity": 0.696, "score": 112.0, "evalue": 9.2e-31, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI00387B1930", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B1930", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "EFPKPENHBP_1896", "locus": "GCA000186005_01892" }, { "type": "cds", "sequence": "contig_1", "start": 1036910, "stop": 1037026, "strand": "-", "frame": 3, "gene": null, "product": "Seryl-tRNA(Ser/Sec) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "nt": "ATGCTTGAACAAGCTAAATCGGTTTTAGAAGAATTAGAAATACCTTATAGAGAATTATTATTATGTACAGGTGACTTAGGATTCTCAAGTAGAAAAACTATTGACTTAGAAGTTTGA", "aa": "MLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEV", "aa_hexdigest": "93f8c13faf2742f3e7b9ef82e59c43b7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920HIT7", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.579, "score": 48.9, "evalue": 6.19e-07, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_A0A920HIT7" }, "pscc": { "uniref50_id": "UniRef50_A0A920HIT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "product": "Seryl-tRNA(Ser/Sec) synthetase" }, "genes": [], "id": "EFPKPENHBP_1897", "locus": "GCA000186005_01893" }, { "type": "cds", "sequence": "contig_1", "start": 1037045, "stop": 1037926, "strand": "-", "frame": 3, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q6KIJ8", "UniRef:UniRef90_C4XFU2" ], "nt": "ATGCTAAATTTAAAATACGTTTTAGAAAATGAAAAGAAAGTTAAAGAAGCTTTAAAAAATAGAAATTTCGATCTAAAGCAATTTAATGAGCTTATTAAAGAAGCAAATAAGCGTGGAAAGCTAATGTTTGAAGCTCAAAGCAAGAAAGCTGAATTAACAAAATTTAGTAAAGAATTTGCAGCTAACAAAAACAATAAAACTAAATTAGCTAAATTACAAAAAGAAGTAGCAAATATTAAAAAAGAACAAGTAGCACTTGAAGAAGCTGCTAAAAATATTGAAAAGAAAGTTAATGATATTTTAATCAGAATACCAAATATTTCAATGGAAGATGTGCCACTTGGTAAAGACGAAAACCAAAATAAAGTTATAAAAAATCATGATAAATTAGGACGTGGATTAGTTTCAGGCGTTCAACCTCACTATGTTATAGCTAAAGACTTAGACATTATCGATCTTGAAAGAGCAGTAAAAATGTCAGGTTCAAGATTTGTAATTTACAAAGGTTTAGGTGCTAGATTAGCTAGAGCTATTATGAATTTTATGTTAGATTTACATACCAAAAATGGTTATGAAGAATATTCGGTACCTGTAATAGTTAAACCTGAAATTCTTTTTGGTACAGGTCAATTGCCTAAATTTAAAGAAGATCTTTTTGCTTTAGAAGATGCTCAAAGTTATTTAATTCCAACAGCTGAAGTCCCTTTGACAAACTACTATAATAATGAAATAATAGATTTATCAAAACCTATTAGAATGACAGCATTTACTGAATGTTTTAGATCAGAAGCAGGAAGTAGTGGAAAAGATACAAGAGGTATTATTAGAAACCACCAATTCAAAAAAGTTGAATTAGTAAAAATTGTTAGTGAAAAAGATTGA", "aa": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKD", "aa_hexdigest": "59c88f385cb38c1536d973d3b1ff70af", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 1036742, "stop": 1037926, "observations": { "mutation": [ 1036912, 1037047 ], "stop": [ 1036912, 1037047 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU2", "query_cov": 1.0, "subject_cov": 0.6926713947990544, "identity": 1.0, "score": 526.0, "evalue": 4.9e-186, "gene_start": 1, "gene_end": 293, "reference_start": 1, "reference_end": 293, "reference_sequence": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKDWEAEFNQMLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEVWLPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK" }, "paralog": false, "identity": 0.9949238578680203, "score": 727.0, "evalue": 2.07e-267, "description": "Internal stop codon at 1036912, 1037047. Nonsense mutation around 1036912, 1037047.", "psc": { "uniref90_id": "UniRef90_C4XFU2", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q6KIJ8", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU2", "EC:6.1.1.11", "UniRef:UniRef50_Q6KIJ8", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIJ8" ], "product": "Serine--tRNA ligase" } }, "id": "EFPKPENHBP_1898", "locus": "GCA000186005_01894" }, { "type": "cds", "sequence": "contig_1", "start": 1038188, "stop": 1038337, "strand": "+", "frame": 2, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02914", "RefSeq:WP_013355004.1", "SO:0001217", "UniParc:UPI0001A8C0FF", "UniRef:UniRef100_C4XFU1", "UniRef:UniRef50_P05647", "UniRef:UniRef90_C4XFU1" ], "nt": "ATGAGTTTAAGAACATATCAACCAAACAAGACCCAACACATCAAGACTCATGGTTTTAGAGCAAGAATGGCAACAACAAATGGTAGAAAAGTTTTAGCTGCTAGAAGAGCTAAAGGTAGAGCAAGACTTACAGTTTCAGATGCTAAATAA", "aa": "MSLRTYQPNKTQHIKTHGFRARMATTNGRKVLAARRAKGRARLTVSDAK", "aa_hexdigest": "5c5a01ca21600f52eea0725329a3cdee", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C0FF", "ncbi_nrp_id": "WP_013355004.1", "uniref100_id": "UniRef100_C4XFU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0FF", "RefSeq:WP_013355004.1", "UniRef:UniRef100_C4XFU1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFU1", "uniref90_id": "UniRef90_C4XFU1" }, "psc": { "uniref90_id": "UniRef90_C4XFU1", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "EFPKPENHBP_1899", "locus": "GCA000186005_01895" }, { "type": "cds", "sequence": "contig_1", "start": 1038435, "stop": 1038686, "strand": "+", "frame": 3, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "SO:0001217", "UniRef:UniRef50_A0A449BAF4", "UniRef:UniRef90_C4XFU0" ], "nt": "ATGATTATTTATTACATGCCTAACGATGATTTCAAAATTGGTATAACTGTACCGAAAAAGTTTTGCAATGCAGTTTTAAGAAATTACTATAAACGCCAATTAAAATCAATTATCCATTCTTTGAATTTGTATGGCGAAAAAATGCATTTTGTCTTAATTCCAAGAAAAGATTTTTTGAATTTAATATATGAAGATAAATTGACTATAACTAAAAAATTATTTGAAAAGATTAAAAATGGAAAACAAAAATAG", "aa": "MIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK", "aa_hexdigest": "8dcf24da0d4ca8bfcb2e97075703a42c", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnpA" ], "truncated": "5-prime", "pseudogene": { "start": 1038342, "stop": 1038686, "observations": { "mutation": [ 1038384 ], "stop": [ 1038384 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU0", "query_cov": 1.0, "subject_cov": 0.7280701754385965, "identity": 1.0, "score": 171.0, "evalue": 8.27e-54, "gene_start": 1, "gene_end": 83, "reference_start": 32, "reference_end": 114, "reference_sequence": "MVYKMKKIYRLQKSWEFDAVIKFQKQVLNKYMIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK" }, "paralog": false, "identity": 0.9824561403508771, "score": 228.0, "evalue": 1.54e-78, "description": "Internal stop codon at 1038384. Nonsense mutation around 1038384.", "psc": { "uniref90_id": "UniRef90_C4XFU0", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A449BAF4", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU0", "EC:3.1.26.5", "UniRef:UniRef50_A0A449BAF4", "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAF4" ], "product": "Ribonuclease P protein component" } }, "id": "EFPKPENHBP_1900", "locus": "GCA000186005_01896" }, { "type": "cds", "sequence": "contig_1", "start": 1038829, "stop": 1039116, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCAAGACCTCAACATATACAGGTGCAGGTACTGAATATTACACATCATACGATAAAATTAGTCCTTATGTAACATCACTTGAACCAGCTAAAGCTGATAAAAAAATCGACAAAGATGACAAGGCTTTACAACCTGGTGAATTCAAGAGAATTGTTAAAAATGATTCTACAAACTACCATGCTAGCTACAAAAATCATGCCGAAATACTTAAAAAGTTAAGAGAGCAAACAGGCGGAGATGTTTATGGAAAATATAACTCATATTCATCTTCTATTCAATGA", "aa": "MVIKTSTYTGAGTEYYTSYDKISPYVTSLEPAKADKKIDKDDKALQPGEFKRIVKNDSTNYHASYKNHAEILKKLREQTGGDVYGKYNSYSSSIQ", "aa_hexdigest": "127ac08dfb145a7c55058a67d232ff9e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10740.811799999998, "isoelectric_point": 8.981364631652834 }, "id": "EFPKPENHBP_1901", "locus": "GCA000186005_01897" }, { "type": "cds", "sequence": "contig_1", "start": 1039210, "stop": 1039866, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCGAGTCTGCAACATATAAACCTACCTACACAACACCTTATAGTGAAATTAAATTTATTGACCCACAATTCAATTTAAATTCAATTTTATCTTTAGTTGATCAAAAAAATCCAGATCTTTATGAAGTTAAAAAAGATCGTTTAGAAAATAAAACATTCAAAACCCCTAAAAAAGCAACATTGTTTTCAATAGCTACACCAGACCAATTGAAAGCAAAAAATAAAATTGCTGGTAAGGAACTTAGTGAAATTGAGCTTTCAAAACAATACTATGCTCGTGATGTTTTAGAATTTTTATATAGAAAAACATTTATTGAACAAGTAAATGGTAAAGATAATTATTTTGCTAGAAGATTCAAACAAGAGTTTGGTACCGATAAATACAATGATGTATTAGGCAAAATAATTGATCCTACAAATACTAACAAATATAGTAAAAAACAAGTGGAACTTATTTATGAATATCTAAATATATTAAATGGATATCTTAAAACTACAGGTTTCACAAATATTTATGACGACACTCAATTAACAAAAGTTGAATTAGCTAAAGGAACTGAAAAAGTTAAAATAGCTAAAGAAAATGCTTATCTTTCTCCTGCTCCAAAACTTGTATACACTCATAGTGAACCATTCAAACCTTTTGTTAAATGA", "aa": "MSESATYKPTYTTPYSEIKFIDPQFNLNSILSLVDQKNPDLYEVKKDRLENKTFKTPKKATLFSIATPDQLKAKNKIAGKELSEIELSKQYYARDVLEFLYRKTFIEQVNGKDNYFARRFKQEFGTDKYNDVLGKIIDPTNTNKYSKKQVELIYEYLNILNGYLKTTGFTNIYDDTQLTKVELAKGTEKVKIAKENAYLSPAPKLVYTHSEPFKPFVK", "aa_hexdigest": "1d8db1bc06cbb4e55aa58926633a9700", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25340.63840000002, "isoelectric_point": 9.24284839630127 }, "id": "EFPKPENHBP_1902", "locus": "GCA000186005_01898" }, { "type": "cds", "sequence": "contig_1", "start": 1040131, "stop": 1040244, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAAGACATCAACAAGAAATTGCGGCTTTATATAAAAAGAATAATATAAATCCTCTTGATGCTTTCTTGAATATAATTATTTCCTTCCCTATCTTCATTGCTATGTGA", "aa": "MKIRHQQEIAALYKKNNINPLDAFLNIIISFPIFIAM", "aa_hexdigest": "b7c63e99f55e16ffee088a48f7a7973d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4330.1669, "isoelectric_point": 9.522834587097169 }, "id": "EFPKPENHBP_1903", "locus": "GCA000186005_01899" }, { "type": "cds", "sequence": "contig_1", "start": 1040776, "stop": 1041114, "strand": "+", "frame": 1, "gene": null, "product": "Nucleoside 2-deoxyribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8", "UniRef:UniRef90_C4XFT8" ], "nt": "ATGAAAAAAATACATGCTTATGTAGCTGGTCCATTGTTTACTAGAGCAGAAATTGACTTAAGATACGCAATCGAAGAAACAATGAAAAAAGCACTAAAATCAAAAGAATTAAAAGGCAAAATTGACTTTGACATTTTCAATCCGATTCATCTAAATGAAGAATTAGAACAAAACGGAAAATTAACACCACAAGAAATTTTCAAAAATGACTTAGCAGCAATTCAAAAAAGTAAACTTACAATTCTTGACATTGACAACAAAGATGATGGCACCATGGCAGAATTTGGTTACTTCTTAGCAATGAAAGAAAGAGATCCAGAAGTTAAAATATGTGTTTGA", "aa": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICV", "aa_hexdigest": "5019ddcd36705ab0d43f0b32dc87cfb5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1040776, "stop": 1041249, "observations": { "mutation": [ 1041112, 1041226 ], "stop": [ 1041112, 1041226 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT8", "query_cov": 1.0, "subject_cov": 0.7133757961783439, "identity": 1.0, "score": 223.0, "evalue": 4.14e-73, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICVWMSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYDWLIKAYK" }, "paralog": false, "identity": 0.9872611464968153, "score": 308.0, "evalue": 3.32e-109, "description": "Internal stop codon at 1041112, 1041226. Nonsense mutation around 1041112, 1041226.", "psc": { "uniref90_id": "UniRef90_C4XFT8", "product": "Nucleoside 2-deoxyribosyltransferase", "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT8", "UniRef:UniRef50_C4XFT8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8" ], "product": "Nucleoside 2-deoxyribosyltransferase" } }, "id": "EFPKPENHBP_1904", "locus": "GCA000186005_01900" }, { "type": "cds", "sequence": "contig_1", "start": 1041115, "stop": 1041228, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATTTTAGAGATGTAGCAGATCGCGACATTAGATTAAACAGATTTATCAACGGAATGATTTATGTATCAGATGGTTGCGTAAAAAATCAACAAGAACTATATGATTGA", "aa": "MSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYD", "aa_hexdigest": "bd75a301a6f1d5c4e7105b81e844b134", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4397.880000000001, "isoelectric_point": 4.658887672424316 }, "id": "EFPKPENHBP_1905", "locus": "GCA000186005_01901" }, { "type": "cds", "sequence": "contig_1", "start": 1041327, "stop": 1041497, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAATAATTTAATCGAAAATAAATTAATTGCTTCAATGCGCGGCATAGCATTGGATTCAATCAACAAAGCAAAAGGTGGCCACATTGGTATGGCTATTGGAGCTGCAAATATTACTTATTCATTATTAGGAAAGGAATTAAGATTCTCAAAAGAAGATCCGAAATGA", "aa": "MKNNLIENKLIASMRGIALDSINKAKGGHIGMAIGAANITYSLLGKELRFSKEDPK", "aa_hexdigest": "6d4df5968824f334d0f4f49936664834", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 49, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 2.7434903931214128e-08, "score": 29.43653106689453, "start": 7, "stop": 55 } ], "seq_stats": { "molecular_weight": 6043.0491999999995, "isoelectric_point": 9.775808525085449 }, "id": "EFPKPENHBP_1906", "locus": "GCA000186005_01902" }, { "type": "cds", "sequence": "contig_1", "start": 1041540, "stop": 1042250, "strand": "+", "frame": 3, "gene": null, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "nt": "ATGTCATATTATTCAATTATGCATTTCTTAGGTTTATTAGCAAAAGAAGACATGCAAAATCATAAAAAAATGGACTCAAAAACACCTTCTCATCCTGAGTTAGAATATACACCTTTTGTGGATGCTTCAACAGGTCCTTTAGGTCAAGGAATAGCTATGGCTACTGGTATGGCTCTTAGTCTAAAAGTACTACAAAACAGGTTTAATCGTAAAAATTACAATATTATAGACCACTACATTTATGCGCTTCATGGAGACGGTTGTATTCAAGAAGGTGTGGCTCATGAAGCTATTCAATTTGCAGGTACAAATCAATTAGATAGACTTATTTTAATTCATGATTTTAATAATGCTCAAATTGATACAAAAACCGAGGTTGTTAATAATATTAACTTCATTAAATATTTTGAAGCTTGTCATTTTGCAACCTTTGTAGTTAAAGAAGATAAACCTGAATTAATTCTTGAAGCAATTCAAAAAGCTAAAGCTTCTAAGAAACCTTCATACATTCAAGTTCACACAGCAATAGCTAAAAATACACCTTTTGAAAACTCGCCAAAAGGTCACCATGGAATGTTAAAAGAAGAAGATACTATTGAATTTAAAAAGAAATGTGGTTTAACTTCATTTGTTCCTTTTGAATATGACAAAGAAGTTTATGACTATGGAGAAGAATTATTAAAAGAAAAAGACAAACATTATAAAGAATGA", "aa": "MSYYSIMHFLGLLAKEDMQNHKKMDSKTPSHPELEYTPFVDASTGPLGQGIAMATGMALSLKVLQNRFNRKNYNIIDHYIYALHGDGCIQEGVAHEAIQFAGTNQLDRLILIHDFNNAQIDTKTEVVNNINFIKYFEACHFATFVVKEDKPELILEAIQKAKASKKPSYIQVHTAIAKNTPFENSPKGHHGMLKEEDTIEFKKKCGLTSFVPFEYDKEVYDYGEELLKEKDKHYKE", "aa_hexdigest": "ecb7c1c3978c878450d8790505a99ccc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF16CA3", "query_cov": 0.809322033898305, "subject_cov": 0.880184331797235, "identity": 0.513, "score": 204.0, "evalue": 6.83e-63, "valid": false, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI002FF16CA3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF16CA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1907", "locus": "GCA000186005_01903" }, { "type": "cds", "sequence": "contig_1", "start": 1042398, "stop": 1043132, "strand": "+", "frame": 3, "gene": null, "product": "transketolase-like TK C-terminal-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "nt": "ATGCAAAAAATAGATCAAAATTACAAAAACGTTTTAGGTGGTTCAGCTGACTTAGCCTCAGCTACAAAAGTTAACTTTAGCAAATCTTATGCTGAAGGTGGCAAAAACATTCACTATGGAATTAGAGAATTTGCTATGACTGCTATAAATAATGGAATCTATTTACACTCACATTTAAGAACAATAGATTCAACATTCTTAGCTTTTAGCGATTATGCTAAAGGTGCTCTAAGACTAGGTTCAATTATGAAAATACCTTCAGTTCATGTTTACACTCATGATAGTTACCAAGTTGGGGGTGACGGACCAACTCATCAACCAGTAGAACAATTAGCAATGCTTAGAAGTACTCCAAATTTTAAAGTTATTAGACCTATGGATGAGAGCGAAGTCAAAGCAGCATTCAATTATGCATTAAATCAAAATGAAAATCAAGTAGCTATTATTGGTTGTCGTCAAAATGTTAAATCTTACAATTTACATCCTGAAGGTGAATTGCAACCAGCTTACATTATTCGTCCTGCAAAAAACCATGACATTAGTATTTTGGCTTCTGGTAGTGAAGTTGACTTAGCAGAAAAGGTTGCTAATAAATTAGTTGGAGCAGGCATTAAAGCTCAAGTTATTTCCGTTCCTTTATTACAAGATTTAGTTAATGATGAAAAATTAATCAAAAGCTTAGGCATTGACAAAAAACCAATATATGCTATTGAAGCTTCAACAGATCCATTGTGA", "aa": "MQKIDQNYKNVLGGSADLASATKVNFSKSYAEGGKNIHYGIREFAMTAINNGIYLHSHLRTIDSTFLAFSDYAKGALRLGSIMKIPSVHVYTHDSYQVGGDGPTHQPVEQLAMLRSTPNFKVIRPMDESEVKAAFNYALNQNENQVAIIGCRQNVKSYNLHPEGELQPAYIIRPAKNHDISILASGSEVDLAEKVANKLVGAGIKAQVISVPLLQDLVNDEKLIKSLGIDKKPIYAIEASTDPL", "aa_hexdigest": "5fe999d8aafce3b0e00b39e66143a0c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080BCD6", "query_cov": 0.9590163934426229, "subject_cov": 0.8447653429602888, "identity": 0.581, "score": 247.0, "evalue": 9.65e-79, "valid": false, "product": "transketolase-like TK C-terminal-containing protein", "uniref50_id": "UniRef50_UPI0026B1FBDD" }, "pscc": { "uniref50_id": "UniRef50_UPI0026B1FBDD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "product": "transketolase-like TK C-terminal-containing protein" }, "genes": [], "id": "EFPKPENHBP_1908", "locus": "GCA000186005_01904" }, { "type": "cds", "sequence": "contig_1", "start": 1044610, "stop": 1045050, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13240.11", "PFAM:PF17032.10", "PFAM:PF13248.11" ], "nt": "ATGGAAGTTGTTAACTCAAGCATTGAAGATATTAGTATACCTAAAGAGCAACAAGATGAATTGAACAAAATTCTTAAAAGAAAAGCCGAAGTTGATATCATGGGAGACGACTATGCTAAAGTTAGAGGCTATGACGTTTACCAAGATGCAGCAAACAATTCGGGTTCAGTAGGAACTTTTTTTGGAGCAGGCATTGGTTTAGGACTAAACGAAGGTGCTTCAAATTTATTAAGTGATAGCAAAATCAATAAATCAGCAACCCCAACAGCAGCAGCTGCATTATTAATCACTTGCGGTACATGTGGAAGCAAAGTATCTAAAGATTCTAAATTTTGTTTTGAATGTGGTTCAAAAATCGCAACAAACAAATGTTCATCATGTAATATCGACTTATCGCCAAATGCAAAATTCTGCGTAAATTGCGGAAAGAAGGTAAAATAA", "aa": "MEVVNSSIEDISIPKEQQDELNKILKRKAEVDIMGDDYAKVRGYDVYQDAANNSGSVGTFFGAGIGLGLNEGASNLLSDSKINKSATPTAAAALLITCGTCGSKVSKDSKFCFECGSKIATNKCSSCNIDLSPNAKFCVNCGKKVK", "aa_hexdigest": "98759b982d361b41e92adc86842ff972", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13240.11", "name": "Zn_Ribbon_1", "length": 23, "aa_cov": 0.15753424657534246, "hmm_cov": 1.0, "evalue": 2.97362266301423e-12, "score": 42.3330192565918, "start": 123, "stop": 145 }, { "id": "PF17032.10", "name": "Zn_ribbon_15", "length": 22, "aa_cov": 0.1506849315068493, "hmm_cov": 1.0, "evalue": 3.00081727654685e-08, "score": 30.724994659423828, "start": 95, "stop": 116 }, { "id": "PF13248.11", "name": "Zn_ribbon_3", "length": 24, "aa_cov": 0.1643835616438356, "hmm_cov": 1.0, "evalue": 3.6741106642443376e-09, "score": 32.15855026245117, "start": 96, "stop": 119 } ], "seq_stats": { "molecular_weight": 15387.314299999987, "isoelectric_point": 7.450250434875489 }, "id": "EFPKPENHBP_1909", "locus": "GCA000186005_01905" }, { "type": "cds", "sequence": "contig_1", "start": 1045499, "stop": 1045597, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACAGATCAAAAATTTAAATCAAGAGAAAGATCACTTAGAGAAGAATACATCACAGGACAAAAACCTAAAGATGAAGAAGGGTTGTACTAA", "aa": "MSKTDQKFKSRERSLREEYITGQKPKDEEGLY", "aa_hexdigest": "a36ef443794295c5669fa644944a48b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3878.2836000000007, "isoelectric_point": 8.065011024475098 }, "id": "EFPKPENHBP_1910", "locus": "GCA000186005_01906" }, { "type": "cds", "sequence": "contig_1", "start": 1045600, "stop": 1045872, "strand": "+", "frame": 1, "gene": null, "product": "Zinc ribbon domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527177.1", "SO:0001217", "UniParc:UPI0001F59952", "UniRef:UniRef100_C4XFT3", "UniRef:UniRef50_C4XFT3", "UniRef:UniRef90_C4XFT3" ], "nt": "ATGGAATGCAAATCATGTGGAGCTAATGTTAGAATCACAGATAAATTTTGTTCTTTTTGCCGATCATTAATCGAAGCACCTAAATCAAGCGTTGAAACATCTCAACACACAACTGCAACTACAAGAGTAGTAACAGAATCAAAAGCAATTAATTATGATGTATTGAACTTATTAGTTATAGAAGATAAATATGACGAAATTGATTTTGATTTATCTTCTAACACAGCTGCAAACTCAATGAAAACATATGTAAACAAAGGAGGAAGCATTTAA", "aa": "MECKSCGANVRITDKFCSFCRSLIEAPKSSVETSQHTTATTRVVTESKAINYDVLNLLVIEDKYDEIDFDLSSNTAANSMKTYVNKGGSI", "aa_hexdigest": "f707bf71c682d1f5a5e771740c35a3b1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F59952", "ncbi_nrp_id": "WP_013527177.1", "uniref100_id": "UniRef100_C4XFT3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59952", "RefSeq:WP_013527177.1", "UniRef:UniRef100_C4XFT3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFT3", "uniref90_id": "UniRef90_C4XFT3" }, "psc": { "uniref90_id": "UniRef90_C4XFT3", "product": "Zinc ribbon domain-containing protein", "uniref50_id": "UniRef50_C4XFT3" }, "pscc": { "uniref50_id": "UniRef50_C4XFT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT3" ], "product": "Zinc ribbon domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1911", "locus": "GCA000186005_01907" }, { "type": "cds", "sequence": "contig_1", "start": 1046032, "stop": 1046184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATATGAATTCAATGATTATCTTAAAGTATTAAAAGATAGAGTATTTAATAAAGAAGGAATTATGCTAAGTGACAGCGACCTACTCGACAAAATAAACTCAGATAGAAACATTGAAGTTTTTGCTATTTGTTCTTATGACTTCTGA", "aa": "MSLYEFNDYLKVLKDRVFNKEGIMLSDSDLLDKINSDRNIEVFAICSYDF", "aa_hexdigest": "83b42b3c4ebb68c8614e94e75906e8b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5928.674499999999, "isoelectric_point": 4.375943183898925 }, "id": "EFPKPENHBP_1912", "locus": "GCA000186005_01908" }, { "type": "cds", "sequence": "contig_1", "start": 1046626, "stop": 1046730, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAAATCAATTTAGTATCAAGATTTATTGATAAAAAAATCAAACTTGAACACTTGTCTTTTGAAAATGAAGTAGAAAAACATGATGCATTAAGACAATGA", "aa": "MNEINLVSRFIDKKIKLEHLSFENEVEKHDALRQ", "aa_hexdigest": "1b6b5d68af35965b1a0d7484f9e95523", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.677600000001, "isoelectric_point": 6.028479957580566 }, "id": "EFPKPENHBP_1913", "locus": "GCA000186005_01909" }, { "type": "cds", "sequence": "contig_1", "start": 1046917, "stop": 1047249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGCTCTAAGTGGCAAAACAGTTTTAAATGAAAAATTTAATGAAATTGTTCAACAAAAAATTGAATTAGAAAAGAAATATGGAGCATTTATTTACTTTGATGTAATTGTTAAAGAAGAAACTTTAACCTTAGATAATTGTGACTACCAAGTTATCATGGAAACATTCAAACCAAATGAAATTGCTAAAATTAATTTAGTTCCTAAAGACTATATTCCAGCTTTTGATCCTTTACCAATTAATAAATTAGAAGAATATGCAATGGAATATAATCCGGGCGGTTCAAAAAATTTAGCAAAAGAAGAATTAAATGAAATTAAAAGATGA", "aa": "MLIALSGKTVLNEKFNEIVQQKIELEKKYGAFIYFDVIVKEETLTLDNCDYQVIMETFKPNEIAKINLVPKDYIPAFDPLPINKLEEYAMEYNPGGSKNLAKEELNEIKR", "aa_hexdigest": "5929d454884129dd65bcfe5a2b6dc57d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12746.611199999985, "isoelectric_point": 4.814740180969238 }, "id": "EFPKPENHBP_1914", "locus": "GCA000186005_01910" }, { "type": "cds", "sequence": "contig_1", "start": 1047445, "stop": 1048041, "strand": "-", "frame": 1, "gene": null, "product": "ribonuclease HII", "db_xrefs": [ "EC:3.1.26.4", "GO:0003723", "GO:0004523", "KEGG:K03470", "RefSeq:WP_013355014.1", "SO:0001217", "UniParc:UPI0001E33051", "UniRef:UniRef100_C4XFT1", "UniRef:UniRef50_I5D6C7", "UniRef:UniRef90_C4XFT1" ], "nt": "ATGTTGAATTATGAAAAAGAAAATTATCCTGACACTAAATTAATTGTAGGCTTAGATGAAGCAGGAAGAGGTTGTTGTGCAGGGCCTTTAGTAGTTGGTTGTGTTATTATGCCTCAAAATTTTAACCATCCTTTAATAAAGGATTCTAAACAATTGAGTCAAAAACAACGTGAGGAAGCGTTAGAAATTATTCTTGAAAATGCTATTGAAACAGTAACAACTATTATTAATGCTCAAGAAGTAGATCAACTAAATCCAAAGCAAGCTTCAAGAATTGGCATGCAAAGGTGTATTAATAAACTTAATACAAAACCTGAATTAGTAATTACAGATTTTGAAAAAATTAATGACATCAATATACCTCAAATTAATTTAGTAAAAGGTGACCAATTAAGTATTAATGTGGCAGCAGCTTCAATTTTAGCTAAAGTAACTAGGGACTTAATTATGGTTGGAATTGACAAAAAATTTCCTATGTATAATTTCAAGAAAAATAAGGGCTATTGTACAAAAGAACATGAAGCAGCAATGAGTCAATATGGCATCTGTGAATTTCATAGAAAGTCATATAAAAATGTTAAAAAATATTTGAATTAA", "aa": "MLNYEKENYPDTKLIVGLDEAGRGCCAGPLVVGCVIMPQNFNHPLIKDSKQLSQKQREEALEIILENAIETVTTIINAQEVDQLNPKQASRIGMQRCINKLNTKPELVITDFEKINDINIPQINLVKGDQLSINVAAASILAKVTRDLIMVGIDKKFPMYNFKKNKGYCTKEHEAAMSQYGICEFHRKSYKNVKKYLN", "aa_hexdigest": "4b51a8bd10e0b19c82873a8af19a91ac", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33051", "ncbi_nrp_id": "WP_013355014.1", "uniref100_id": "UniRef100_C4XFT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33051", "RefSeq:WP_013355014.1", "UniRef:UniRef100_C4XFT1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFT1", "uniref90_id": "UniRef90_C4XFT1" }, "psc": { "uniref90_id": "UniRef90_C4XFT1", "product": "ribonuclease HII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_I5D6C7", "kegg_orthology_id": "K03470", "go_ids": [ "GO:0003723", "GO:0004523" ] }, "pscc": { "uniref50_id": "UniRef50_I5D6C7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D6C7" ], "product": "Ribonuclease HII" }, "genes": [], "id": "EFPKPENHBP_1915", "locus": "GCA000186005_01911" }, { "type": "cds", "sequence": "contig_1", "start": 1048095, "stop": 1048655, "strand": "-", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0", "UniRef:UniRef90_C4XFT0" ], "nt": "TTGTTCTCAATTATTAGAAACATTAGATCACTAGCTACTGCTTACAATCCAATTATTTTATCAATAACAATAGTTACTTCAGTTATCACAGTTGTTTTAATTGTTTTCCAATTCTTGTTTGTTAGTCAATTAAGATACTTCAAAGGTGTATTTATTAATGCAAGAAGATTAGAAGAAATCAAACAACTTCAAAAAAAGATGAAAGAATCAGGTATGATGGATGAAAGCAATTTAGCAAACAATCTTTTTGGAATCATGTTAGATGATGTTGATGATGTAAGTGCTAATCAAGATAAAAGCAAATTCAATAAAGAAGAAGATATTGAAGAAGCTGAAATAGTTGATAACAAAGCACATCTTAAAAATAATGAAAAAGAAAATGAAGAAAAAATCAAAAAATTATTAGCATTGCCTAATGCAAAACTTTACAACATAGCAGAAACCCTATACATTAGTGGTTACAAAAAAATGGACAAAGAAGAATTATGCCGCTTAATTGTTCAAATTGTTGATACTCAAAAGGCTAAAGAAGACGAAGAAGCAAACAAAGACAAAAAATAA", "aa": "MFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKMDKEELCRLIVQIVDTQKAKEDEEANKDKK", "aa_hexdigest": "6f9b50f0732cacbd0a937a46baf315c2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1048095, "stop": 1048955, "observations": { "mutation": [ 1048667, 1048694 ], "stop": [ 1048667, 1048694 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT0", "query_cov": 1.0, "subject_cov": 0.577639751552795, "identity": 0.995, "score": 329.0, "evalue": 2.11e-111, "gene_start": 1, "gene_end": 186, "reference_start": 137, "reference_end": 322, "reference_sequence": "MDIGEKMNLFDKKYLQIKSTNKLWALEKERFRPVVILSLLTFFLALACALTSTILLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHTWPSMVLIISWFINLFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKMDKEELCRLIVQIVDTQKAKEDEEANKDKK" }, "paralog": false, "identity": 0.993006993006993, "score": 539.0, "evalue": 1.06e-196, "description": "Internal stop codon at 1048667, 1048694. Nonsense mutation around 1048667, 1048694.", "psc": { "uniref90_id": "UniRef90_C4XFT0", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT0", "UniRef:UniRef50_C4XFT0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "EFPKPENHBP_1916", "locus": "GCA000186005_01912" }, { "type": "cds", "sequence": "contig_1", "start": 1048692, "stop": 1048901, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCGCTGCTTTTAAAGATAATTTAGTTGACTATATTGTAAAAATTAATGGTGGTACAACTCCAAGTTTTAAACCTGAAGATCAAATAATGAGCATGATTATTACAAATGGTTTAGTTACAGTTGGTATGTTAGTTAGCATTATTGTATTTTTAGTTTTTTATGCTCGAACATTTAAAGAAAAATCATTAGCCAACATGCATACATGA", "aa": "MLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHT", "aa_hexdigest": "62d54fabd05170d1bf1e5153377c760e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7685.072599999998, "isoelectric_point": 8.085834312438966 }, "id": "EFPKPENHBP_1917", "locus": "GCA000186005_01913" }, { "type": "cds", "sequence": "contig_1", "start": 1049072, "stop": 1049995, "strand": "-", "frame": 3, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_013527180.1", "SO:0001217", "UniParc:UPI0001F59955", "UniRef:UniRef100_C4XFS9", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_C4XFS9" ], "nt": "ATGTTAAAAATTAGTGTTCAATATAAAGAGAGAATCGATAAGTATATCGGTCAAAATAGTGAATTAAGTCGAAATGATATTAAAGAGTTAATTGAGCAAGGTGCAGTTTATATTAATGACAACGTTCAAGTACATAAATGTAATTACATCGTTCGCGAAGGACAAGAAATTACAATTGTTAAATTACTTAACAAAGAAATTAAAATTGAACCAGTTAATATTCCTCTTGACATTAAATATGAAGATGATGATTTAGTAATAATTAATAAACCAAGTAATCTAGTTGTCCACCCTTCACCGGGGCATTACAATGACACACTAGTTAATGGACTTTTATATCATTTTAAAAACAATTTATCAAATGAAAATGGGTTACTTCGACCAGGTATAGTACACCGTATTGATAAAGACACAAGTGGCTTATTAATAATTGCTAAAAATAACAAGATGCATAACTCATTAACTGACATGCTCAAAAGACATGAAATTGAAAGAAGTTATTTAGCTATCGTTAGAGGCTTAATTGAAAATCCACTTACTAAAATTAATTTACCAATTGCTAGAAATACTGTTGATCGTAAAATGATGTCAGTTCATAAAGAAGGTAAAGAAGCTATAACCTTTGTTCATTTGCTTAAGCACTTTTATTTAGATAAAGAACCAATGTCTTTAGTTAGATGCGAATTAAAAACAGGTCGAACTCATCAAATTAGAGTCCACTTGTCTTACATCAAACATCCAGTTTACGGTGATCCTACTTATGGCAAAAGTGTTGATGAATTTAATCAAAGATTACATGCTTATAAATTAAAATTTATTCATCCACTTACTAAAAAAGAAATTTGTGTTTATGCACCGGTGCCTAAAGAATTTGATGTAGCCGATTATGATTTTAATGAACTATTAAAAAATAATGTTTTATAA", "aa": "MLKISVQYKERIDKYIGQNSELSRNDIKELIEQGAVYINDNVQVHKCNYIVREGQEITIVKLLNKEIKIEPVNIPLDIKYEDDDLVIINKPSNLVVHPSPGHYNDTLVNGLLYHFKNNLSNENGLLRPGIVHRIDKDTSGLLIIAKNNKMHNSLTDMLKRHEIERSYLAIVRGLIENPLTKINLPIARNTVDRKMMSVHKEGKEAITFVHLLKHFYLDKEPMSLVRCELKTGRTHQIRVHLSYIKHPVYGDPTYGKSVDEFNQRLHAYKLKFIHPLTKKEICVYAPVPKEFDVADYDFNELLKNNVL", "aa_hexdigest": "49276f710a7a9fb172f4c119c7722451", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59955", "ncbi_nrp_id": "WP_013527180.1", "uniref100_id": "UniRef100_C4XFS9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59955", "RefSeq:WP_013527180.1", "UniRef:UniRef100_C4XFS9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFS9", "uniref90_id": "UniRef90_C4XFS9" }, "psc": { "uniref90_id": "UniRef90_C4XFS9", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [], "id": "EFPKPENHBP_1918", "locus": "GCA000186005_01914" }, { "type": "cds", "sequence": "contig_1", "start": 1050177, "stop": 1050440, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAACTTATTTAGTCTTTGTTATTCCACCAATTTTAATGATGAAATTAGCAATGAAATATTCAAAAAAATTTGAAAATAATAAATATTTAATAAAATTAATTAACATAATGAATCCTGTTGTAACAGGTATTATTGTTGCCTTGGGTTTTCAACTTTTTATAGCTTCAGTAGCACCTCAAGTTTTCTTTAATAAATCATTTACTAACTATGTAGGAATAAATAATGATACACAAAAAGCACTGTTTTATAGGGGTTGA", "aa": "MFLTYLVFVIPPILMMKLAMKYSKKFENNKYLIKLINIMNPVVTGIIVALGFQLFIASVAPQVFFNKSFTNYVGINNDTQKALFYRG", "aa_hexdigest": "ff114127c916604c6f5209d78b9669e8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10004.98719999999, "isoelectric_point": 9.932079887390138 }, "id": "EFPKPENHBP_1919", "locus": "GCA000186005_01915" }, { "type": "cds", "sequence": "contig_1", "start": 1050701, "stop": 1050907, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGTAATAATTATGGTTCTAATTATTTGTATTTTTGCAACTGTTGAAGCAATCAAACACAAAAAAGAAATCAAAAAACTTAAAGAAAATATTGAAAATAAAGTTACTAATTTAGACAATAACATAGAAGAAAAAATAGAACAAAACTTAGAATTAGAGACCAAAGAAAATGATTTGAATAGTGACAAGAAAGGAGGTCAATAA", "aa": "MPVIIMVLIICIFATVEAIKHKKEIKKLKENIENKVTNLDNNIEEKIEQNLELETKENDLNSDKKGGQ", "aa_hexdigest": "e56a278954b69d1ea5da6add22e1ddca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7834.0481999999965, "isoelectric_point": 5.3715917587280275 }, "id": "EFPKPENHBP_1920", "locus": "GCA000186005_01916" }, { "type": "cds", "sequence": "contig_1", "start": 1050965, "stop": 1051231, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCAGGTCCTAGCGTCATTGAAGCAATTAGTTATATTACTATTAAACTTTTAGGTTTCAAAAAGGGCTTGTTTTTGGCAACTTTTGCAACCTTACCTCATGTTTTAGTTTTCTTTTTAATCTTTTATTTTGTGCAACATATTCCTCAAAAATATTTGTTTGTTATTGAACTTGGTGTTATTGTTGCAATTATTGGATCATTGATTGGTTTTGCTTGGGAATACTTCAAAAAATCAAGAGGCAAAACTAAAATTAGTTTGTGA", "aa": "MLPGPSVIEAISYITIKLLGFKKGLFLATFATLPHVLVFFLIFYFVQHIPQKYLFVIELGVIVAIIGSLIGFAWEYFKKSRGKTKISL", "aa_hexdigest": "1faedfa2cefe365f13dafcceb95d05f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9952.00599999999, "isoelectric_point": 9.853041648864746 }, "id": "EFPKPENHBP_1921", "locus": "GCA000186005_01917" }, { "type": "cds", "sequence": "contig_1", "start": 1051591, "stop": 1051830, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAAAAAATGTTTATCAATTGCAAAGAGAAAAAATATTAAAAAATGCTTTTATTGACAAAAAATTGAGAAAAGAAAATATCGAAAAACTTTACTCAATAATTGTGCAAAATGAAAGTAAAATTAAAGAAGCTTTATCTTTGGACCTTAATAAATCATCACTAGAATCATATTTTAGTGAGATAGGTTTAGTCTTAAAAGAAATCAAATTTAATATTAAAAATTTAAATAAATGA", "aa": "MNAKNVYQLQREKILKNAFIDKKLRKENIEKLYSIIVQNESKIKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNK", "aa_hexdigest": "c0a666b852bfeb01ff9cc9b2984644c7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9301.804799999993, "isoelectric_point": 9.619085884094236 }, "id": "EFPKPENHBP_1922", "locus": "GCA000186005_01918" }, { "type": "cds", "sequence": "contig_1", "start": 1051954, "stop": 1052547, "strand": "+", "frame": 1, "gene": null, "product": "Aldehyde dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS6", "UniRef:UniRef90_C4XFS6" ], "nt": "ATGCCATTAGTTTCAGCTCTTGCAGCAGGCAATAGAGTAATCATTAAACCAAGTGAATTTTCAGTCCACACTTCACAATTATTACAAGAATTAATCAACAATAATTTTGATCCTCAATATGCTTTTGTAGCAGACAGTTCTTTAGAAGTTGCTCAAGAATTGTTGGACTACAAATTTGATTTAATTCATTTCACAGGTAGCACAACAGTTGGAAAAATTATTGCTAAAAAAGCATCTGAAACTTTGACACCAATTACTTTAGAATTAGGTGGGAAGTGCCATTGCGTTGTTGACAAAAGTCAAGACATAATTTATTCTGCTCAAAAAATTATGGCTGGTAAATTGCTTAATGCAGGTCAAACTTGTATAGCTCCTGATTATGTTTTAATTGATAAAAATAGAATTAACGCATTTAAAGCTGCTTGTTATGTTTATCTTGAAAACGTTTTGCAAAATGAAGAATATATTAAAGGTAATTATCCAAAGATAATTAATCAAAAACATTTTGAAAGATTAAAAAAATATTTACCTAAAGATCATAAAATTAATGAAAAAACATTACAAATAATGCCTTATATTTTTGAAACAAGCTGA", "aa": "MPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCHCVVDKSQDIIYSAQKIMAGKLLNAGQTCIAPDYVLIDKNRINAFKAACYVYLENVLQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETS", "aa_hexdigest": "715ef86e2de3a6e144babdfeb0284d00", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1051654, "stop": 1052850, "observations": { "mutation": [ 1051828, 1051927, 1052545 ], "stop": [ 1051828, 1051927, 1052545 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS6", "query_cov": 1.0, "subject_cov": 0.41041666666666665, "identity": 0.995, "score": 386.0, "evalue": 1.4e-131, "gene_start": 1, "gene_end": 197, "reference_start": 153, "reference_end": 349, "reference_sequence": "MRSKFKITYKFNIIFITKLFIYNILRQTGDIMNAKNVYQLQREKILKNAFIDKKLRKENIEKLYSIIVQNESKIKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNKWTKPFHVSTPSHLALGKSEIQYEPLGQVLIISPWNYPFYLSLMPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCPCVVDKSQDIIYSAQKIMAGKLLNAGQTCIAPDYVLIDKNRINAFKAACYVYLENVLQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETSWNDLIMQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEFWIKKIIK" }, "paralog": false, "identity": 0.9899497487437185, "score": 774.0, "evalue": 1.02e-286, "description": "Internal stop codon at 1051828, 1051927, 1052545. Nonsense mutation around 1051828, 1051927, 1052545.", "psc": { "uniref90_id": "UniRef90_C4XFS6", "product": "Aldehyde dehydrogenase", "uniref50_id": "UniRef50_C4XFS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS6", "UniRef:UniRef50_C4XFS6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS6" ], "product": "Aldehyde dehydrogenase" } }, "id": "EFPKPENHBP_1923", "locus": "GCA000186005_01919" }, { "type": "cds", "sequence": "contig_1", "start": 1052560, "stop": 1052919, "strand": "+", "frame": 1, "gene": null, "product": "aldehyde dehydrogenase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1", "UniRef:UniRef90_UPI0002E0F0E1" ], "nt": "ATGCAAGAAGAAATTTTCGGGCCGTTGTTGCCTATTATTCCTTATGATAATTTAGATGATTTATTAGTCAAATTTAAATCACTTCCTAAACCTTTAGCTGCTTATTTATTTACTAAAGATAAGAAAATAATAAAGCTATTTAAAGAACAAGTTACTAGTGGAGCTTTAGTAATTAATGATACCATTATGCATATAGCAAATAGTAATTTACCTTTTGGTGGTGTTGGTGATTCTGGCTATGGCAGAAGTCATGGTTTTGAAGGTTTTAAAGCTATGTCAAATCCAAAAGCTATTTACCAAAGAAAAGGTTTTAATTTTAAATTAAATGAGCATCCTTACAGCAAGAAAAAAGAATTCTGA", "aa": "MQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEF", "aa_hexdigest": "1ba18871fb632d394b157164d06658a4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1052401, "stop": 1052940, "observations": { "mutation": [ 1052545, 1052917 ], "stop": [ 1052545, 1052917 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "query_cov": 1.0, "subject_cov": 0.664804469273743, "identity": 1.0, "score": 242.0, "evalue": 2.76e-80, "gene_start": 1, "gene_end": 119, "reference_start": 54, "reference_end": 172, "reference_sequence": "MQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETSWNDLIMQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEFWIKKIIK" }, "paralog": false, "identity": 0.9832402234636871, "score": 358.0, "evalue": 1.58e-128, "description": "Internal stop codon at 1052545, 1052917. Nonsense mutation around 1052545, 1052917.", "psc": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "product": "aldehyde dehydrogenase family protein", "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002E0F0E1", "UniRef:UniRef50_UPI0002E0F0E1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1" ], "product": "aldehyde dehydrogenase family protein" } }, "id": "EFPKPENHBP_1924", "locus": "GCA000186005_01920" }, { "type": "cds", "sequence": "contig_1", "start": 1053034, "stop": 1053312, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5", "UniRef:UniRef90_C4XFS5" ], "nt": "ATGTTGCCTTATATTATTGTTGGCTACAGTCATGAAAAATTAAAATATTTTTCGCATGTCAATATATCGTTAGGAGTAATCTTAATTATCTTAACTATTTCGCAAGTAGCAGTTTCAACTAAAGCAAATTTCCTTATTTATTTCTTAATAAGCTTTGAAATAATTAACGTTATTTGCATAACTATTAGTTTGATTTTTGGCATTATTTTCTTTTTTGATTATCTACAAAGTTTCAAAGAATCTTTATTAATATTTAAACAATATTTAAAAGAACTTTAA", "aa": "MLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL", "aa_hexdigest": "dcdbed1e550841e45b35f9fdb5193f66", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1053034, "stop": 1053456, "observations": { "mutation": [ 1053366, 1053435, 1053438 ], "stop": [ 1053366, 1053435, 1053438 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS5", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 167.0, "evalue": 1.3e-51, "gene_start": 1, "gene_end": 92, "reference_start": 49, "reference_end": 140, "reference_sequence": "MNKQKRWWMLLSYVIIGLVLLIINVVLVCCWQKGSGVFISLIGQTILVMLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL" }, "paralog": false, "identity": 0.9785714285714285, "score": 258.0, "evalue": 7.23e-90, "description": "Internal stop codon at 1053366, 1053435, 1053438. Nonsense mutation around 1053366, 1053435, 1053438.", "psc": { "uniref90_id": "UniRef90_C4XFS5", "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS5", "UniRef:UniRef50_C4XFS5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10704.786499999991, "isoelectric_point": 7.852652168273926 }, "id": "EFPKPENHBP_1925", "locus": "GCA000186005_01921" }, { "type": "cds", "sequence": "contig_1", "start": 1053885, "stop": 1054451, "strand": "+", "frame": 3, "gene": null, "product": "Glucokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4", "UniRef:UniRef90_C4XFS4" ], "nt": "ATGGCTTTGGCAAATCACAAATATTACAAACAAAAGAAAAGTGATGTAACTCAATTTTTCACAGTATCAACAGGTTTAGGTGCTGGTCTTATTATTAATGATCAAATATATGTTGGTAAAAATCACTTAGCTCAAGAAATCGCGAGAGCACCTTTAGGTACTGATGAAGATGACTCATTTCATTTACTTCCTTATTCAGTGGAATTGTATGCTTCAGGAACAGGCATTGAATTAAGATATAACAAATTAACAGGAGAAAAATTAAACGCTAAAAAAATTTTTGCTTTATATCCAAATAATGAAAATGCAAAAAAAATAATAAATGAAGGTATAGATTCTTTAGCTAGAACTATCTCAACTTCTTTAGCTTTTTTAAATCCAAGTCTTGTTGTTTTTGGCGGTAGTGTTAGTGAACATAATAATTGGTTTATAGAAAAAGCTATTGAAAAAGCTAAAACATATACAATTAAATCTCAATATCAAAATGTAAAATTTGCTTTTGATCCTTATACAGATTTCAGCGCTGTAATGGGTTTATATTGTTTAGCAAAAGATAAATTTAAATAA", "aa": "MALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK", "aa_hexdigest": "bb9384e04443ebc48cca139096dc696e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1053585, "stop": 1054451, "observations": { "mutation": [ 1053639, 1053804 ], "stop": [ 1053639, 1053804 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS4", "query_cov": 1.0, "subject_cov": 0.6394557823129252, "identity": 1.0, "score": 375.0, "evalue": 6.9e-130, "gene_start": 1, "gene_end": 188, "reference_start": 107, "reference_end": 294, "reference_sequence": "MMKIATADIGGTNTRFALFKKNKFWSKVKFETNKNNYRETLNHIAHLCNLHKIECLALCIPGPADYDKGIVLNTPNLPGWVNKNIKKYLFKHTNLKEIITENDANVMALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK" }, "paralog": false, "identity": 0.9930555555555556, "score": 577.0, "evalue": 3.43e-212, "description": "Internal stop codon at 1053639, 1053804. Nonsense mutation around 1053639, 1053804.", "psc": { "uniref90_id": "UniRef90_C4XFS4", "product": "Glucokinase", "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS4", "UniRef:UniRef50_C4XFS4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4" ], "product": "Glucokinase" } }, "id": "EFPKPENHBP_1926", "locus": "GCA000186005_01922" }, { "type": "cds", "sequence": "contig_1", "start": 1055214, "stop": 1055615, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTATGAGTATGACAAACATTAGCAGAAAGAAGAAGATGAAAAAATATTTTTTGAATAGATTTATTTTAGGTAGTGCTTTATCTTTTGCTCCAATAATATCTGCTTCTTGTGTTCAAAAACAAGAAGTTCCTTTTGAAGACAAATTAATTGAATCAAAATATGAAAATAGTTCAATAATTGGCTTAAAGGACAAAGATTTACTTAAATTAAATTTAAATCAAATAAAGTATTTTATCGAGCAAACTAATAAATTAAGATTAAAAAATAATTTTGAACTAAAACAAGTTGTAAAAGAAAATGATAATCTATTTTTGTCAATAAATAATAAAAAATATTCATTAAAAAGTTTTTTAAAATCAGTTAATGATAATTGGGATATATTATCAAAAAATTGA", "aa": "MVFMSMTNISRKKKMKKYFLNRFILGSALSFAPIISASCVQKQEVPFEDKLIESKYENSSIIGLKDKDLLKLNLNQIKYFIEQTNKLRLKNNFELKQVVKENDNLFLSINNKKYSLKSFLKSVNDNWDILSKN", "aa_hexdigest": "8205a24575aa2e93087e14736b5e351d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15622.147399999987, "isoelectric_point": 9.760078239440915 }, "id": "EFPKPENHBP_1927", "locus": "GCA000186005_01923" }, { "type": "cds", "sequence": "contig_1", "start": 1055679, "stop": 1055849, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTTTGAAAATGATAATTCAAATTTTAAAAATTTAGGCTATGTTAATTATGTTAAAAGATTGTTTAAATCAAAATACAACAATTTAGCAAATGCTTCTAAAAATTTACCTGATTTACAAATGATTTTACAAGTTGCAATTTCAGGAAGTAATAGATTTTCAATTTGA", "aa": "MFFENDNSNFKNLGYVNYVKRLFKSKYNNLANASKNLPDLQMILQVAISGSNRFSI", "aa_hexdigest": "062c32007a20141a52ceb6b1ffa0ed39", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6478.3287, "isoelectric_point": 9.775808525085449 }, "id": "EFPKPENHBP_1928", "locus": "GCA000186005_01924" }, { "type": "cds", "sequence": "contig_1", "start": 1056132, "stop": 1056773, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTCAAGATTCTAATCCTAGATATCATGCATTTCAAAAATATGGAAATTTTGAAATCGATTCTTCATTAGAAACAAATGAAAAAAGCAAATCTTTATTTAATGAAATGTTTAAGGAACCAACACTTTCATTTGAAAAAAATATGTTCAATTTTAATGATTTTGATTCATTAATGCACCCTTCAAAACCTTATTTAGGTTTTAATCTTAATTCTTTTATGCAATTCTATAACGCTTATAAAGAAAAGGTCAAAATTAATGTTCCTGATGAAAATTATGAAGCAAAAATAAAAGAAATAAAATTTGACAAAATATTAAATAATTCACATTCAATAGGCAAGGCTATAGTTGAAATTAAAAACAAAAAAACTAATAGAGTACAAGAAGCTAATTGGTTTACTATTGACTTTACATATGCACATAAACATATATTTGATGGCTTTTATATTCAAAATAAATTAGATTGTGATCCTTTAAAAAATGATGAATTCTTCAGCTATTATATAAATAAAGGAATTGGAACAAATAATGAATTAAAATTACCTGAAGGCATTAATGCAGAAGAATTTTATAACACTAATTTAATAGATATTGTTAACTTTATTATTTATCAAAACAGAGATTCATTTAACATTTGA", "aa": "MDFQDSNPRYHAFQKYGNFEIDSSLETNEKSKSLFNEMFKEPTLSFEKNMFNFNDFDSLMHPSKPYLGFNLNSFMQFYNAYKEKVKINVPDENYEAKIKEIKFDKILNNSHSIGKAIVEIKNKKTNRVQEANWFTIDFTYAHKHIFDGFYIQNKLDCDPLKNDEFFSYYINKGIGTNNELKLPEGINAEEFYNTNLIDIVNFIIYQNRDSFNI", "aa_hexdigest": "a3ecff2df08ff550db9451bd6511186c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25323.087500000023, "isoelectric_point": 5.438718605041504 }, "id": "EFPKPENHBP_1929", "locus": "GCA000186005_01925" }, { "type": "cds", "sequence": "contig_1", "start": 1056786, "stop": 1057178, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGTTCTAGAAATAATTCAAAATAAAGCAGAATTTGAAAAAAAAATAAGTACATTAATTTCCCAATATGTCCTTAAATTCTTTATAAACAACAAAAAACCAGGTTATGAAGAATTAATAAAAGAAGTTAAAGTTACTATTGATCCAAATAATGATTATGACGCCTTAAAATATGGCGTCGGTAAGTTACCAATTAGAATAGATTTCATTAATCATAAAAATGAAGATATGATTAAAAACATAAGTGAAAGAAAATTCTTATTAGGCGGTTTTGTTGGTTATGATTTCAGTGATATTGAAGCAAAATTAAAAACATTAACTGATGCTGATTTTGAGAGACCTCTTAAGGATAAAACTCCACCTTATTTAATCAAAAACTAA", "aa": "MNKNSVLEIIQNKAEFEKKISTLISQYVLKFFINNKKPGYEELIKEVKVTIDPNNDYDALKYGVGKLPIRIDFINHKNEDMIKNISERKFLLGGFVGYDFSDIEAKLKTLTDADFERPLKDKTPPYLIKN", "aa_hexdigest": "61d09685d3a716907af0f5a65c5049bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15118.308199999983, "isoelectric_point": 8.630850028991702 }, "id": "EFPKPENHBP_1930", "locus": "GCA000186005_01926" }, { "type": "tRNA", "sequence": "contig_1", "start": 1057214, "stop": 1057303, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 68.1, "nt": "CGGTAGTTGCTCGAGAGGCTGAAGAGGTTGGTCTCGAAAACCAATAATGGTGAAAGTCATTCAAGGGTTCAAATCCCTTACTACCGGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 1057290, 1057292 ], "id": "EFPKPENHBP_17", "locus": "GCA000186005_01927" }, { "type": "cds", "sequence": "contig_1", "start": 1057344, "stop": 1057640, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTGCCCTTAATTTCTATGGTTGGTATTGCAATTATTGCTATACCTGGTGGTATGGTAGCAGCAAGTGTTTTAACTAGTTTTCAAAAGAAAAAAGATAATAAAGAAAAAGCTAATGAGCTTGCTGAAATAATTGATGCAGAAATTGAAAGAAGAGTTAAAGAAAGAATTAAAGAAGAAGAAAAAGAAATGAAACACGATTTATTACATCGTTTTGATGAAACAATTCAAAAAGAAAAAGAATTAAAACACACAAACGAACACGCATCTCATGATCATAAAGTTCATAAATAA", "aa": "MIVPLISMVGIAIIAIPGGMVAASVLTSFQKKKDNKEKANELAEIIDAEIERRVKERIKEEEKEMKHDLLHRFDETIQKEKELKHTNEHASHDHKVHK", "aa_hexdigest": "8fdb3a81a51ce436363347d41ad2629b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11301.969799999988, "isoelectric_point": 6.656209754943849 }, "id": "EFPKPENHBP_1931", "locus": "GCA000186005_01928" }, { "type": "cds", "sequence": "contig_1", "start": 1057848, "stop": 1058117, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTACAAGCGGCAATATTATTTTGATTCTTACATTTTTGCCTTCACTTTATGTGATTAATAATTTCTTGCCTGAAGCTAATCAAAGTAATGCATTAAAAGCTTTTAACGGCTTTAAGTTTTTAAGAATTTTAAGATTAATTATGCTTTTAAATGTTTTTGTAGTTTTTAAAAATATTTCAGAATCTGTATCAAATGAAAAAGTTTTGTTTTTCAATATCTTCTTATTTATAGGTATTTTGATCGTTTTATTTGCTCTTACAGTTTGA", "aa": "MITSGNIILILTFLPSLYVINNFLPEANQSNALKAFNGFKFLRILRLIMLLNVFVVFKNISESVSNEKVLFFNIFLFIGILIVLFALTV", "aa_hexdigest": "155106bb445b572ee9f6aef75110c8be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10128.15609999999, "isoelectric_point": 9.819711494445798 }, "id": "EFPKPENHBP_1932", "locus": "GCA000186005_01929" }, { "type": "cds", "sequence": "contig_1", "start": 1058346, "stop": 1058471, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAGAAACTAACTTAATAAAAAATAAGAAAATAAATGACATTTTTGAAGCAATTGGAATAATTGTTACAGTTGATAACGATTTAAAAACAAACAACAAAAAACATAGAATTACATTATGA", "aa": "MERETNLIKNKKINDIFEAIGIIVTVDNDLKTNNKKHRITL", "aa_hexdigest": "de5678d6c1aca33bd754db253ab59e4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4794.5322, "isoelectric_point": 9.301836967468262 }, "id": "EFPKPENHBP_1933", "locus": "GCA000186005_01930" }, { "type": "cds", "sequence": "contig_1", "start": 1058800, "stop": 1058946, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACATAATTAATACAGCTCGTGGTTTCTTAGAAAAATATCAAGAAGATGCTTTATTTAAAGAATGTATTTTGAATAATGCTGATGCTAGGGATGAAATTTTTGCTTTTGGTTTTTTAAGAGATACAAGAAGCAATTCGTGA", "aa": "MSNIINTARGFLEKYQEDALFKECILNNADARDEIFAFGFLRDTRSNS", "aa_hexdigest": "a403fecf85cf8ba49e1f15ef2b3a0434", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5546.124199999998, "isoelectric_point": 4.7658586502075195 }, "id": "EFPKPENHBP_1934", "locus": "GCA000186005_01931" }, { "type": "cds", "sequence": "contig_1", "start": 1059080, "stop": 1059325, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAGATGAATCTACCAAATAAACTAACAATACTTAGATTGATTTTATTCTTACCTCTTTTAGCTCTTTTTATTACTTATAAGATGATCCAAAATTACATGGATAATTACTATAAAATAGCTGGCCGTTCAATATTATCTGGAATTTTACTTATCTTTTTAGGAGCAATGATTACTGACTATTTTGATGGCAAAATTGCTAGAAAATACAATATGATTACAAGCTTTGGAAAGCTGTGA", "aa": "MTKKMNLPNKLTILRLILFLPLLALFITYKMIQNYMDNYYKIAGRSILSGILLIFLGAMITDYFDGKIARKYNMITSFGKL", "aa_hexdigest": "698cabfcd0d8447851f1d1cf11f03082", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9417.429299999993, "isoelectric_point": 9.99648380279541 }, "id": "EFPKPENHBP_1935", "locus": "GCA000186005_01932" }, { "type": "cds", "sequence": "contig_1", "start": 1059500, "stop": 1059721, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCTTGTTTCAAACGCTTGCAATTATTGCTATTTTAATTATTGCTATAAGCTTTGAATATCCTATTGAATTGGTTGAAAAATATGGCCTAACTTTAGTTCAAAGTGATGCTTGTATTTATGCAATTAACACTCTTACAATTGTTGCATTATTCTTCAGTATTTACTCAGGAATATTGTATGTTAAAAAAATAATCCCTTTCCTAGAAATGGTATAA", "aa": "MKTLFQTLAIIAILIIAISFEYPIELVEKYGLTLVQSDACIYAINTLTIVALFFSIYSGILYVKKIIPFLEMV", "aa_hexdigest": "1da5b8764598b52dae3e7412251de1b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8249.945299999998, "isoelectric_point": 5.002422523498535 }, "id": "EFPKPENHBP_1936", "locus": "GCA000186005_01933" }, { "type": "cds", "sequence": "contig_1", "start": 1059760, "stop": 1060047, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355028.1", "SO:0001217", "UniParc:UPI0001E3305D", "UniRef:UniRef100_C4XFR8", "UniRef:UniRef50_C4XFR8", "UniRef:UniRef90_C4XFR8" ], "nt": "ATGAATAAAGATGACAAAAAAGAAAACAGCTTAGAGATTACAACTCTATACGATGCTGAAAAATTTAGAATTATTAAGAATGAAGATTTAAACAATTTAACTAATGAACAAAGAGAAAAATTAGTTAAAGATGCAATTGATAATGCTAAAAAAAATGAAGCCAATATAATTGCAAATAGGAAGAAAAGATATTTGTCTTCTTTAGCTGTAATTTTAGTAGCTGGAATAATCATTGCAATTACAGTAATTTTGATACTTGCACTAATTATTTACTTACATAAAAAATAA", "aa": "MNKDDKKENSLEITTLYDAEKFRIIKNEDLNNLTNEQREKLVKDAIDNAKKNEANIIANRKKRYLSSLAVILVAGIIIAITVILILALIIYLHKK", "aa_hexdigest": "b47f11197ed18bc1e6b5968d894f0d86", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3305D", "ncbi_nrp_id": "WP_013355028.1", "uniref100_id": "UniRef100_C4XFR8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305D", "RefSeq:WP_013355028.1", "UniRef:UniRef100_C4XFR8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR8", "uniref90_id": "UniRef90_C4XFR8" }, "psc": { "uniref90_id": "UniRef90_C4XFR8", "uniref50_id": "UniRef50_C4XFR8" }, "pscc": { "uniref50_id": "UniRef50_C4XFR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10891.683399999989, "isoelectric_point": 9.362372779846194 }, "id": "EFPKPENHBP_1937", "locus": "GCA000186005_01934" }, { "type": "cds", "sequence": "contig_1", "start": 1060082, "stop": 1060504, "strand": "-", "frame": 3, "gene": null, "product": "NAD-dependent protein deacylase", "db_xrefs": [ "EC:2.3.1.286", "GO:0034979", "GO:0051287", "GO:0070403", "SO:0001217", "UniRef:UniRef50_C4XFR7", "UniRef:UniRef90_C4XFR7" ], "nt": "TTGGATATTAAAGCTGGTAATAAATATGTAATTGAATTACATGGAAATATTAATCGTAATTATTGTTTGCTCTGTGGTAAGTTTTATGATTTAGCTCAATTAATTCATCAAAAAGATAAAGATGGAATTCCTACTTGTAAATGTGGGGGAGTAATAAATCCAGATGTAGTTTTATATGAAGATCCACTAATGGAAGATTCAACTAATGATGCAGCTGAAGCCATTTCAAATAGTGATTTATTAATTATTGCAGGTACTTCATTAAGTGTTTATCCTGCAGCTTCATATATTCATTTTTATCAGGGTAAAAGAATAGTAATTCTAAATAAAGATACTTCACGTTATGAAAATAGTAACAATGAAAATATCTTGTTGTTTAATGAAAATATGAAGGATGTTTTTGAAAATTTAGCTAATTCATAG", "aa": "MDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS", "aa_hexdigest": "92cf2e5d29258527de8c46031b4582df", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1060082, "stop": 1060804, "observations": { "mutation": [ 1060552, 1060648, 1060795 ], "stop": [ 1060552, 1060648, 1060795 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR7", "query_cov": 1.0, "subject_cov": 0.5577689243027888, "identity": 0.993, "score": 281.0, "evalue": 1.52e-94, "gene_start": 1, "gene_end": 140, "reference_start": 112, "reference_end": 251, "reference_sequence": "MVQNFNEKIDKFKNWIKESKHIVFFSGAGVSTASGIPDFRSADGLYSKKFKNMNPESILSRSFWRKNKKDFYEYYFSNIAFDNIKPNIIHETVASWCNKNKCHVITQNIDNLDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS" }, "paralog": false, "identity": 0.9875, "score": 480.0, "evalue": 2.61e-175, "description": "Internal stop codon at 1060552, 1060648, 1060795. Nonsense mutation around 1060552, 1060648, 1060795.", "psc": { "uniref90_id": "UniRef90_C4XFR7", "product": "NAD-dependent protein deacylase", "ec_ids": [ "2.3.1.286" ], "uniref50_id": "UniRef50_C4XFR7", "go_ids": [ "GO:0034979", "GO:0051287", "GO:0070403" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR7", "EC:2.3.1.286", "UniRef:UniRef50_C4XFR7", "GO:0034979", "GO:0051287", "GO:0070403" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR7" ], "product": "protein acetyllysine N-acetyltransferase" } }, "id": "EFPKPENHBP_1938", "locus": "GCA000186005_01935" }, { "type": "cds", "sequence": "contig_1", "start": 1060680, "stop": 1060868, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAATTTTTTAGAATATAAACCATCAGCTGATCTAAAATCAGGAATTCCTGAAGCTGTTGAAACACCAGCTCCACTAAAGAAAACAATATGTTTAGATTCTTTAATTCAATTTTTAAATTTGTCTATTTTTTCATTAAAATTTTGCACCATATACCTCTATTTAATTTTAGCAAAAATATAA", "aa": "MFLNFLEYKPSADLKSGIPEAVETPAPLKKTICLDSLIQFLNLSIFSLKFCTIYLYLILAKI", "aa_hexdigest": "c841bf6945028a5052c8f73c8ce29cab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7041.417999999996, "isoelectric_point": 7.743994331359863 }, "id": "EFPKPENHBP_1939", "locus": "GCA000186005_01936" }, { "type": "cds", "sequence": "contig_1", "start": 1061146, "stop": 1061457, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4", "UniRef:UniRef90_A0AB32XDE4" ], "nt": "ATGAGATTAAGAAATGACAAAGATGCACCAAAAAAGCTTGAAGAATCTAATTTATTGATTACTAAAAGTCAATATCCTATTAGCTTAAAAAATACTGATGTAATTGAAATTGGCATGGGTAAAGGCGAAATGATAACTCAATTAGCTGCTCTAAATCCAAATATAAATTTTTATGGTTTAGAAAAGTTTGCTACTGTAGCTGCTAAAAGCGTAAGAAGAGCTAAAGAATATAAGCTTAATAACTTTAAAATTATTTTAGAAGATGCTAAAGAATTAAAAAGCATTTTTAAAGGAACTTGTGGCACAATTTGA", "aa": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELKSIFKGTCGTI", "aa_hexdigest": "a859b06ff65da50b67809ff42c1d2200", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1061038, "stop": 1061457, "observations": { "mutation": [ 1061127, 1061148 ], "stop": [ 1061127, 1061148 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDE4", "query_cov": 1.0, "subject_cov": 0.7410071942446043, "identity": 1.0, "score": 199.0, "evalue": 3.14e-64, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELKSIFKGTCGTIWLTFSDPWPKARHFKRRLTYKDFLKNINLLWIKILF" }, "paralog": false, "identity": 0.9856115107913669, "score": 270.0, "evalue": 1.43e-94, "description": "Internal stop codon at 1061127, 1061148. Nonsense mutation around 1061127, 1061148.", "psc": { "uniref90_id": "UniRef90_A0AB32XDE4", "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDE4", "UniRef:UniRef50_A0AB32XDE4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" } }, "id": "EFPKPENHBP_1940", "locus": "GCA000186005_01937" }, { "type": "cds", "sequence": "contig_1", "start": 1061565, "stop": 1061759, "strand": "+", "frame": 3, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355031.1", "SO:0001217", "UniParc:UPI0001E3305F", "UniRef:UniRef100_C4XFR5", "UniRef:UniRef50_C4XFR5", "UniRef:UniRef90_C4XFR5" ], "nt": "ATGGAATTTAAAGACAAAATTTTACGTGCTAGAGCTCTTTTAAACTTAACTCAAGAAGAATTAGCAAAACAACTTGGTGTTTCTTTTAACACCATAAATAGATGGGAAAATGGAAAAGTTAAGCCAACAAAAGTTGCTAAATTGAGATTTGATGAATATTGTAGAAAAAATGGTGTTTTGTTGGAGGTTAGATAA", "aa": "MEFKDKILRARALLNLTQEELAKQLGVSFNTINRWENGKVKPTKVAKLRFDEYCRKNGVLLEVR", "aa_hexdigest": "22909f2efd67cb793c157f1fa02e3864", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E3305F", "ncbi_nrp_id": "WP_013355031.1", "uniref100_id": "UniRef100_C4XFR5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305F", "RefSeq:WP_013355031.1", "UniRef:UniRef100_C4XFR5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR5", "uniref90_id": "UniRef90_C4XFR5" }, "psc": { "uniref90_id": "UniRef90_C4XFR5", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_C4XFR5" }, "pscc": { "uniref50_id": "UniRef50_C4XFR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR5" ], "product": "HTH cro/C1-type domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1941", "locus": "GCA000186005_01938" }, { "type": "cds", "sequence": "contig_1", "start": 1061759, "stop": 1062232, "strand": "+", "frame": 2, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "nt": "ATGGAATTTAATGAAGATACAAGAGTAAAATTACCAGCAACGATTCACTTTTTAAAACTTGGTTACAATTATCAAACTGTTAGAAGCCCAGAAATTAATAGAGACAATAACATTTTTGAAGAAAGATTTCATAGAGCAATTGAAAAAATAAATAAGAAAAAATTTGCTAAAGAAGAAATAAATGAATTGATCTCTGATATAAGCAGAAAAATCAAAAATAAGGATTTAGGAAAAGAATTTTATAATCGATTAATTTCGACCGATTACCCTATAAAACTGATAGATTTTGAAAATATTGAGAACAATGATTTTGCAATTGTTAATGAATTAACTTTTAGAAGTAACGCTAATTCTAATTATGATTCATTTAGACCAGATATTACAATTTTAGTTAATGGTATGCCATTAGCATTTTTAGAAGTAAAAGTTAAAAACAACAAAAATGGTATTCAAGCAGAATTTATTAGAATGTGA", "aa": "MEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRM", "aa_hexdigest": "5a44f6c11b8f9e40968808b5677e8983", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_R5XCY4", "query_cov": 0.9872611464968153, "subject_cov": 0.9935897435897436, "identity": 0.568, "score": 167.0, "evalue": 1.4e-50, "valid": false, "product": "type I site-specific deoxyribonuclease", "uniref50_id": "UniRef50_R5XCY4" }, "pscc": { "uniref50_id": "UniRef50_R5XCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "product": "type I site-specific deoxyribonuclease" }, "genes": [], "id": "EFPKPENHBP_1942", "locus": "GCA000186005_01939" }, { "type": "cds", "sequence": "contig_1", "start": 1062275, "stop": 1062523, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATTGTTTCATTTACAAACAATATGGATTATGAAGAATTTAATGATGCTGAGGCTTCACTACTAAAACAAGGCTCTTTCTATACAACACCAAATGGAAAAGACAATACTACTTTTTCAGCTTTTAGAGAAGATGAACCTAACTACCATGCTAACTACTCTTTTGAGGAAATAGACAATGACAAAATAAAAGAAATTTTAATTGATAATAATATTAAAACTTCACATGTTGATAGTGATTGA", "aa": "MLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSD", "aa_hexdigest": "ef0950a75bc7fd9a519371e59a491703", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9492.098799999992, "isoelectric_point": 4.251465797424316 }, "id": "EFPKPENHBP_1943", "locus": "GCA000186005_01940" }, { "type": "cds", "sequence": "contig_1", "start": 1062617, "stop": 1062778, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTATCTTAGCCAAATAAAAGAAATTAAAAATGAAACTTTAAATAAAGTTGAACAATTGCCAATTAATCAAAAACACATTATGAGATATCCTCAATTTTTCGCTACAAGAGCAATTATCAAAAGATTCAAACGAACTAATAAAAATGGAATTATTTGA", "aa": "MMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGII", "aa_hexdigest": "5b21485bac4e6ec319f5f9f95330c7c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6436.6222, "isoelectric_point": 10.509910011291506 }, "id": "EFPKPENHBP_1944", "locus": "GCA000186005_01941" }, { "type": "cds", "sequence": "contig_1", "start": 1062899, "stop": 1063549, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACAAGCTTCTGCAGAATTTGCCAATAGAGGTTTCAATGTTGTTAATGTTGATTCAAAAGAAGCATTCGCTAAAGAATTAAATGAGACAATCGATAGAAATAATAATGGTATTGGCACAATTTGTGTTGTAAACATTCATAAATTTATGGATAGTTCAAAAATGCCAGAAGCTAAAAATGATTACAACATAAGAACTCAAAGAATTTTCTTTATTGATGAAGCTCACAGAAGTTATACAATGCATGGTGAATTTTACAAAAACCTTATGACTTGTGATAGAGATGGAATTTACATTGCAATGACTGGTACACCTTTATTAACTAAAAGAGAAAGATCAAATTTAAAATTTGGAGACTACATTCATAAATACTTTTATGATAAATCAATAGCTGATGGTTATACTTTGAGAATTAAAAAAGAACAAATTAAAACTGAAGCTAGAGCTACAATTATTAGAAACTTAAATATTGAAATTGAAAAATTAGAATCAAGAATTATTTATGAATCAGATGATTATGTTAAAAGCGTTAGCCAATATATAGAAAAAGACTTTATTGATTTTAGATTTATTTGCAGAAATGATTTAACAATTGGTGGAATGATTGTCTGTCGTTCAAATGACCAAGCGAAAAAAATTCATGAATGA", "aa": "MRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHE", "aa_hexdigest": "dc1ee2318cc28d86bb1673fd40c0666c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25291.487800000028, "isoelectric_point": 8.23810863494873 }, "id": "EFPKPENHBP_1945", "locus": "GCA000186005_01942" }, { "type": "cds", "sequence": "contig_1", "start": 1063607, "stop": 1064854, "strand": "+", "frame": 2, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01153", "SO:0001217", "UniRef:UniRef50_C4XFR4", "UniRef:UniRef90_C4XFR4" ], "nt": "TTGCAAGACAACATAAATAAAAATAATCAAAAAACCTTTAAAGAAAGTAATTATCCAGATATTTTAGTTGTTCACTATATGCTTACAACTGGATATGATCTTAAAAGACTTAAGAAGATGTATTTATTAAGAGGACCTAAAGCTCAAAATCTTTTGCAAACTATTTCACGTGTTAATAGACCCTATCGTAGTCCTAATGGCCAAGAATATCAATATGGATATATAGTTGATTTTATTGATATTGAAAAAGAATACAACAATACTATTGATGCTTATCGTAAAGAAATAGAAGAAGAATACGATTTTGAAGAAGAAGACACTTCCCTTTCTGGTTTAATTATTGATAAAACAGATATCAAAAAAAGATATGATGTTTTAAAAAGTGATCTTGAAAAATTTGTTAATAATTACAATTTAGAAACCTTTGTTGACAAAATTAACAATTTTGACAAAGATACTTTACAAAAGATCAAAAAGATTCTTGTTAATATTAAAAACTGTTCAATTGAATTCAAATTATCACAAGCTGATGAATATTCAAAACTCATTAACGATTATGAAATCAATGTGTTTATTAAAGAAGTACAAAAGAAAATTAATTTTTTAAACTTGCGTTCAGATCCTATTGATACAATTCAATTAATAGATAACAAAGAAGTTGTTCAAATCATTTATGATTTTCTTTTAAAAAATATTACTGTTTTAGATTTAGGTAAATTTGATAAAGAAAATAAAATGGAACTTATTAAACAAAAGTTTATGAAAATGCAAAAAGAAATTAAAGAAAACAAAAATACAAGAGATGTTAAATTTATGAATCTTAATGCATTGATTAAGAAATTACTTGATAAATTAGCTGTTGAGGAATTGACTTACGAAGAAGTAGAAGATGAATTAGATTTAATTTACAATTCAACTAAAAAAATAAATGAAGAAAATAAAGAAATATCTAAAGACTTTGGAAATAGTTTGGCTTTTGTCACAACAATGAATATTGTTTTAGCAGAAACTAAATTAGATCGTTCAATTTTAAAGAACTTTTTATTAATGGTTTATGATGAATTAAAAGGCAACATTTCTGACGAAATCCTCATTAAACAAGGCAAAAATGGATTTATTGACTCAACAAAATCTAAAATAACAACTAGATTAATTAAGAAGAATTTATATAAACAAATTAAAGATAATTACGATTATATTTTGAACGAATTATACTTTAATCTGCTTAATTACAAGGAAACACTTTAA", "aa": "MQDNINKNNQKTFKESNYPDILVVHYMLTTGYDLKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL", "aa_hexdigest": "a589002a3b0c88e665bbcaa94bf86ff3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1063307, "stop": 1064854, "observations": { "mutation": [ 1063547 ], "stop": [ 1063547 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR4", "query_cov": 1.0, "subject_cov": 0.4021317829457364, "identity": 0.995, "score": 788.0, "evalue": 7.12e-278, "gene_start": 1, "gene_end": 415, "reference_start": 618, "reference_end": 1032, "reference_sequence": "MMEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRMWEDRLKVDEYRKFFNMLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSDWFQTNLSDTTPCNRFITSIFDKERFLYFIKYGMMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGIIWHTQGSGKTALSAFATKVIKDYWSKKKINARFFFIVDRLDLMRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHEWFKKNSKLTTGLVLSDTDNPLQDNINKNNQKTFKESNYPDILVVHYMLTTGYDVKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL" }, "paralog": false, "identity": 0.996116504854369, "score": 1006.0, "evalue": 0.0, "description": "Internal stop codon at 1063547. Nonsense mutation around 1063547.", "psc": { "uniref90_id": "UniRef90_C4XFR4", "product": "type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR4", "kegg_orthology_id": "K01153", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR4", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR4", "KEGG:K01153" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR4" ], "product": "type I site-specific deoxyribonuclease" } }, "id": "EFPKPENHBP_1946", "locus": "GCA000186005_01943" }, { "type": "cds", "sequence": "contig_1", "start": 1064863, "stop": 1065867, "strand": "+", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "nt": "ATGACTAATTTTACAAAACTTGAAAATAATATAAAATCAATAATCGATGATTTGAAGGGATTGTGTTCAACAAATGGTCTTTCAAATACAGCTGGCGAAGAAATAGTTGTTACTTCTGTCTTTTTATATAAATTTCTAAATGATAAATTCATTTCAAATTTAAAAAAATTTGCAAAAGAATTAAATGTTCCTTTTGAAGAAATAATTAAAAATTCAAACTATATGGATGGATTTTATGATGCTTATCCACAAGATGTTGCATTCAAATATGAAGATACAATTGATTATTTAGTTGGATACATTAATGAAGATGATTTTTATCAAAAATTTGACGATACATTAATTAGAATTTCAGATTATAAACAAAATGAAGCTTTTAATGTTGATACTGCAGAAGGAACTAAAAAGCCACTTTTTGTTCGTCTTAGTACTTTTGTTGAACCTTCAAAAAGAAATAACTTCACAAAAAATGTTTTTTCTTACATTACAAGAGATAGATTTGATTTTGGTGAAGCTTTTAATAATAACTATGATTTCTTTTCAACTATTTTTGAATACCTAATTTCAGATTACAACATTGCAAGTGGTAAATATGCTGAATACTTTACACCTCAAACATTATCAAAAGCTATTGGTGAAATATTAGTTAAAATGTCTCCAATTGAAGATAAAATTTATGAAATATATGACCCAAGTGCAGGTTCTGGATCGCTTGTGTTGCATTTAGCAAATGAACTTGGAGAAGGTAAATTTGGAAATAAGGCTCGTGTTTACACTCAAGATATTTCACAAAAATCATCAAGATTTTTAAGAATAAATATGTTACTTAATGGTTTAAAAGAATCACTAGATAACATAATTGAAGGCGATACCTTACTTACCCCAACACACTATAAAAAAGCAGGAGACGCAACAACAGGTCTTAAACAATTTGATTTTATTACATCTAATCCACCATTCAAAACTGACTTCTCTTCTACTAGAGATAACATTGAATTAATGTGA", "aa": "MTNFTKLENNIKSIIDDLKGLCSTNGLSNTAGEEIVVTSVFLYKFLNDKFISNLKKFAKELNVPFEEIIKNSNYMDGFYDAYPQDVAFKYEDTIDYLVGYINEDDFYQKFDDTLIRISDYKQNEAFNVDTAEGTKKPLFVRLSTFVEPSKRNNFTKNVFSYITRDRFDFGEAFNNNYDFFSTIFEYLISDYNIASGKYAEYFTPQTLSKAIGEILVKMSPIEDKIYEIYDPSAGSGSLVLHLANELGEGKFGNKARVYTQDISQKSSRFLRINMLLNGLKESLDNIIEGDTLLTPTHYKKAGDATTGLKQFDFITSNPPFKTDFSSTRDNIELM", "aa_hexdigest": "871ae26b538784c51a21bc31cda63678", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378PT86", "query_cov": 0.8083832335329342, "subject_cov": 0.9926470588235294, "identity": 0.6779999999999999, "score": 379.0, "evalue": 1.44e-129, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A378PT86" }, "pscc": { "uniref50_id": "UniRef50_A0A378PT86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "EFPKPENHBP_1947", "locus": "GCA000186005_01944" }, { "type": "cds", "sequence": "contig_1", "start": 1065928, "stop": 1066074, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTATCTTTTATTTATTCAACATATTCTTTATTCATTAAAAGAAGGTGGTAAAGCCGCTATAGTTGTACCTACAGGGTTTATTACAGCACAAAGTGGAATTGAAAAAACTATTAGACAATATATTATTGATAGAAAATGA", "aa": "MAIYLLFIQHILYSLKEGGKAAIVVPTGFITAQSGIEKTIRQYIIDRK", "aa_hexdigest": "c27f2ab7268c6135ce0229476ee18b4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5409.3904999999995, "isoelectric_point": 9.600454521179199 }, "id": "EFPKPENHBP_1948", "locus": "GCA000186005_01945" }, { "type": "cds", "sequence": "contig_1", "start": 1066093, "stop": 1066518, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATCAAATATTTTTGCTAATACTGGAACTAACGTATCCGTTTTATTTATAGATAAAACAAATACTAATGGTGAAGTGTTATTAATGGACGCTTCAAAAATGGGTCATAAAGAAAAAGTTAAAGATCTTCAAAAAACAGTTTTAACTCATGATGAATTAAACAAAATAGTTAATGATTTTGTTAATCATAAAGTTGAAGATGATTTTACAATTTCAGTTACCTATGATCAAATAAAAGAAAGAAACTATTCATTATCAGCTGGTCAATATTTTGAAGTAAAAATTGAATATGTTGATATTACTCAAGAAGAATTTGACAAAATGATTGATGACTTTAATAAAGAATTAGAACAATTTACAAAAGATACAGAAGAATTCCAAAAAGAGATGTCTAAAACATTGAAGGAGTTGAAGCTAAAATAA", "aa": "MPSNIFANTGTNVSVLFIDKTNTNGEVLLMDASKMGHKEKVKDLQKTVLTHDELNKIVNDFVNHKVEDDFTISVTYDQIKERNYSLSAGQYFEVKIEYVDITQEEFDKMIDDFNKELEQFTKDTEEFQKEMSKTLKELKLK", "aa_hexdigest": "2384f6fe1c63022b714c5656a9d56b25", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16470.382100000003, "isoelectric_point": 4.8395788192749025 }, "id": "EFPKPENHBP_1949", "locus": "GCA000186005_01946" }, { "type": "cds", "sequence": "contig_1", "start": 1066535, "stop": 1066651, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAAATTAAAAGAATTAGGTATATTTGAAACTGGAAGCACATTAATCAAAAAAATTGGAAATTTTCCTGCTTTTGGAGGCAATGGCATTATTACATATGTGAATAAATGA", "aa": "MEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNK", "aa_hexdigest": "1b63389fc682220f1b547564c3e65f25", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.872100000002, "isoelectric_point": 9.224861717224119 }, "id": "EFPKPENHBP_1950", "locus": "GCA000186005_01947" }, { "type": "cds", "sequence": "contig_1", "start": 1066775, "stop": 1067011, "strand": "+", "frame": 2, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR2", "UniRef:UniRef90_C4XFR2" ], "nt": "ATGGCAAGATTTTATTTTTATTTTCTTTTACATTTAAACTTAAATAAATTTCATATAGGTTCTTCACAGCCTTTATTAACTCAAGGAATTTTAGGAAACATAAAAATAAATATTCCTAGTTTAAATAAGTGCCAAAAAATATCAAAAATATTAGATAACATAGATAATCAAATTGAGCGAAATAATTCTATGGTTCAAAAGTTACAAGTTATGGGACAGGCTATTTTTAATAGGTGA", "aa": "MARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQVMGQAIFNR", "aa_hexdigest": "3080f7494af61fbd6fd5691b983d0d81", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1066520, "stop": 1067002, "observations": { "mutation": [ 1066649 ], "stop": [ 1066649 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR2", "query_cov": 0.9743589743589743, "subject_cov": 0.4418604651162791, "identity": 0.934, "score": 138.0, "evalue": 3.52e-40, "gene_start": 1, "gene_end": 76, "reference_start": 86, "reference_end": 161, "reference_sequence": "MIRINMEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNKWNVDEDAIIIGRVGANCGCVNITNKKSFVTDNALIFKPKEKNMARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQSFEQALIFSKKWGANYAC" }, "paralog": false, "identity": 0.9625, "score": 309.0, "evalue": 7.93e-110, "description": "Internal stop codon at 1066649. Nonsense mutation around 1066649.", "psc": { "uniref90_id": "UniRef90_C4XFR2", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR2", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR2", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR2", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR2" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "EFPKPENHBP_1951", "locus": "GCA000186005_01948" }, { "type": "cds", "sequence": "contig_1", "start": 1067276, "stop": 1067653, "strand": "+", "frame": 2, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR0", "UniRef:UniRef90_C4XFR0" ], "nt": "TTGAAAAAAGGTACTGTAGTTATTTCTATAACAGGAAATATAAGGGTCAGTTATCTTGCAATTGATTCATGTATAAATCAATCTATAGTAGGAATAGAAGAAAATGAGTTATTGAAAATAGGATATTTATATCCTTTTTTAAAGAATAAAATTGAGTTTTTAATAAGATCTTCTACTGGGAACTGTCAAAAACATATAAATAAAAATTTTATAGAAAATTTAAAAATTGTTTTACCACCAAAAAATGTTTTGGACATATTTAATAATTTAACTCAAAATATATATGCTAAAATAAGTCAAATCAGTTTAATGACTAAGAAATTAATAAAATTCAAAAATAAGCTATTACCTTTATTGATTAATCAACAAATTATTTAA", "aa": "MKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII", "aa_hexdigest": "f1bf1e76eae9959ca484347e32675609", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1066976, "stop": 1067653, "observations": { "mutation": [ 1067009, 1067090, 1067165, 1067183 ], "stop": [ 1067009, 1067090, 1067165, 1067183 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR0", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.992, "score": 237.0, "evalue": 1.09e-77, "gene_start": 1, "gene_end": 125, "reference_start": 101, "reference_end": 225, "reference_sequence": "MQVMGQAIFNRWFLQFEHFKKDNKFKYNEDLNLKIPENWEVKKIAEICKIFLGGTPSTKNREYWNGEINWLNSGEVANFPIIDSEKTINEKGLKNSNTKLLKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII" }, "paralog": false, "identity": 0.9777777777777777, "score": 430.0, "evalue": 5.03e-156, "description": "Internal stop codon at 1067009, 1067090, 1067165, 1067183. Nonsense mutation around 1067009, 1067090, 1067165, 1067183.", "psc": { "uniref90_id": "UniRef90_C4XFR0", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR0", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR0", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR0", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR0" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "EFPKPENHBP_1952", "locus": "GCA000186005_01949" }, { "type": "cds", "sequence": "contig_1", "start": 1067677, "stop": 1068477, "strand": "-", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR1", "UniRef:UniRef90_C4XFR1" ], "nt": "ATGTTTCTTTATTATCTTTTAATGTATATGACACCTTATTGGCTTTCATCAACTAATGGATCAATAATGATTCATATTACAAAAGAAACAATGGAAGAAAAAATTGTTGATTTGCCTCCACTAAAAACTCAAAAAAAGATTTCTAAAATATTAGAAAATTTAGACAAGCAGATTGAAAAGAATTTGCATATAGTCAAAAGATTACAAGTTATGGGACAGGCTATTTTTGATATGTTTTTGAATAATGCGAAAGATTATGAAAATATAGAAAGTTTGTGCAAAATAATTTGGGGTCAGTGTCCAAAAGGAAACAACATTTTAAGCGAAAACGTATCAAATAATTTAATGCTTTATGCATCAGGAGCAGGTGATTTAGAGAATAATAAAATATTAATTTCTCCTAAAGCTTTTACTGATAAACCAATTAAAATAATAGATAATAGGACAATTTGTATGTCTATTGCGGGAACTGTTGGTAAAATTGGAATAAGTGATAAAAATATTGCAATTGGGAGAGCTATGGTTGGATTTTATAATGAAAAAAAATTTGGACTAATTTATTTTATTCTAAATAAATATAGTTCATTTTTAAAAAGACAATCAATTGGAGCTATACAAAAAATTATTAACAAAAATCATCTCAATATTGTGAATGTTCCAATATTAACAAACGAAAAAAACAATTTATTAAACGAATTAATAACTAAATGCATGAAATTAGAAAAAAATACTTTAAGTTTGATAAAGCTTAAAGAAAAGTTGATACCTTTATTAATAAATGGCCAAATAGAAATAAATTAG", "aa": "MFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN", "aa_hexdigest": "2772fdf5fd13c481cc24e4e0aff158a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1067677, "stop": 1068768, "observations": { "mutation": [ 1068501, 1068513, 1068576, 1068642 ], "stop": [ 1068501, 1068513, 1068576, 1068642 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR1", "query_cov": 1.0, "subject_cov": 0.7327823691460055, "identity": 1.0, "score": 508.0, "evalue": 3.92e-180, "gene_start": 1, "gene_end": 266, "reference_start": 98, "reference_end": 363, "reference_sequence": "MMLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHIWKIKWDEKRVDKMFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN" }, "paralog": false, "identity": 0.9862258953168044, "score": 712.0, "evalue": 4.66e-263, "description": "Internal stop codon at 1068501, 1068513, 1068576, 1068642. Nonsense mutation around 1068501, 1068513, 1068576, 1068642.", "psc": { "uniref90_id": "UniRef90_C4XFR1", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR1", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR1", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR1", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR1" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "EFPKPENHBP_1953", "locus": "GCA000186005_01950" }, { "type": "cds", "sequence": "contig_1", "start": 1068640, "stop": 1068765, "strand": "-", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826", "UniRef:UniRef90_UPI000FF44826" ], "nt": "ATGCTTGTTAAATTGAAAGATATTGTAACTTTCATTAATGGAAGAGCCTATTCTCAACCTGAATTACAAGACAAGGGAAAATATAGAATAGTGCGAGTAGGAAATTTTTCTGGTAAAAATGAATGA", "aa": "MLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNE", "aa_hexdigest": "7a0be707c383d09657e1b0ce1df1f77d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1068511, "stop": 1068765, "observations": { "mutation": [ 1068576, 1068642 ], "stop": [ 1068576, 1068642 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44826", "query_cov": 1.0, "subject_cov": 0.4880952380952381, "identity": 0.976, "score": 83.2, "evalue": 4.73e-20, "gene_start": 1, "gene_end": 41, "reference_start": 1, "reference_end": 41, "reference_sequence": "MLVKLKDIVTFINERAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHI" }, "paralog": false, "identity": 0.9642857142857143, "score": 170.0, "evalue": 7.94e-57, "description": "Internal stop codon at 1068576, 1068642. Nonsense mutation around 1068576, 1068642.", "psc": { "uniref90_id": "UniRef90_UPI000FF44826", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44826", "UniRef:UniRef50_UPI000FF44826" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826" ], "product": "restriction endonuclease subunit S" } }, "id": "EFPKPENHBP_1954", "locus": "GCA000186005_01951" }, { "type": "cds", "sequence": "contig_1", "start": 1068827, "stop": 1069399, "strand": "-", "frame": 3, "gene": null, "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "db_xrefs": [ "KEGG:K14060", "RefSeq:WP_013527190.1", "SO:0001217", "UniParc:UPI0001F5995E", "UniRef:UniRef100_A0AB32XD76", "UniRef:UniRef50_C4XFQ9", "UniRef:UniRef90_C4XFQ9" ], "nt": "ATGATTTATGGTTATACAAGAGTTAGCACAAAAAAACAAATTGATGGATATGGTTTTGAAATACAACAAAATGAAATATTGAATAAATATCCAAACGCAATAATAATTAATGAACAATTTACAGGAACAAAATTAAATAGACCACTATTTAATGATCTATTAACTAAATTAGAAAAAGATGATATTCTAGTAGTTTCTAAATTAGATAGATTTGCTAGAAATACAGTTGAAGGTATTAAAGTAGTTGAAGCTTTATTTAAAAAGAAAGTAGCAATACATATTTTAAATATAGTCTTATTAGAGGACACTCCAATGGGTCGTTTTTTCTTAACAACTATGTTAGCAGTTGCTGAATTAGAAAGAAACATGATAGTAGAAAGAACACAAAATGGTAAAGAGATAGCAAGACTAAGAACAGATTATAAAGAAGGTAGACCAAAAGTTTATACTGAAGATCTTTTAAGTGAAGCAATTTTGCTTTTAGATTCCTTTTCCTATCGCCAAGTTTCACTTTTAACTGGTATTAGTAAATCAACATTAGTTAGAGCTAAAAAAGCACTAAAAAATAGCTAG", "aa": "MIYGYTRVSTKKQIDGYGFEIQQNEILNKYPNAIIINEQFTGTKLNRPLFNDLLTKLEKDDILVVSKLDRFARNTVEGIKVVEALFKKKVAIHILNIVLLEDTPMGRFFLTTMLAVAELERNMIVERTQNGKEIARLRTDYKEGRPKVYTEDLLSEAILLLDSFSYRQVSLLTGISKSTLVRAKKALKNS", "aa_hexdigest": "6460bc7fc69b56821b5948f0a4cf3906", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F5995E", "ncbi_nrp_id": "WP_013527190.1", "uniref100_id": "UniRef100_A0AB32XD76", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5995E", "RefSeq:WP_013527190.1", "UniRef:UniRef100_A0AB32XD76" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD76", "uniref90_id": "UniRef90_C4XFQ9" }, "psc": { "uniref90_id": "UniRef90_C4XFQ9", "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XFQ9", "kegg_orthology_id": "K14060" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ9" ], "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1955", "locus": "GCA000186005_01952" }, { "type": "cds", "sequence": "contig_1", "start": 1069563, "stop": 1070537, "strand": "+", "frame": 3, "gene": null, "product": "ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_A0A3P8KMA1" ], "nt": "ATGGATTTTAATTTAGATGACTTATTTGGTTCTAATGACAATAAAAGTAAAAAATCAAGTGATCCATTAAAAATTTATGGAAGAAACTTAACTGATTTAGCTGCTCGGAATGAATTAGATCCTGTTATTAATCGTGATGATGAAATCAGAAGAATGATTCGCATTTTGAGTAGGAAAACAAAAAACAACCCAGTATTAGTCGGCGAACCAGGTGTTGGTAAAACAGCTATTGTTGAAGGATTGGCTAGAAAAATAGTTGAGGGTCAAGTACCAGAAAACTTAAAAGGTAAAGATATTTTTGAACTTGACTTAGCTGCTTTAATAGCTGGCGCTTCATATCAAGGACAATTTGAAAAGAGATTAAAAGACGTACTCAAAAGAATTGAAGAATCTAATGGTGACATTATAGTTTTCATTGATGAAATTCACATGCTTATTGGCACAGGTCGAAATGCTGAAGGTGGTATGGATGCTGCTAACATCATTAAACCTTTAATGGCCAGAGGTAAAATGCATTTAATTGGTGCTACAACTTTTGATGAATATAGAAAATATATAGAAAAAGATGCGGCTCTTGAAAGAAGAATGCAAAGAGTGGATGTATTAGAGCCTTCAATTGAAGATACTATTACAATTCTTCGTGGTATCAAAGACCGTTTTGAAAATTACCACAATGTAAAAATTCAAGATGATGCTTTAGTAGCTGCTGCTCGTCTTTCTTCAAGATATATTTCTGATAGATTTTTACCAGATAAAGCTATTGACTTAGTTGATGAAGCTGCTGCAACTATTAAAACAGAAATAAACTTTCAACCTGAAGAATTAGAAAAAGCTAAACAAAAAATGGCTAGATTGAAAATGGAAAAAATAGCCATTTCTAGTGAAGACAAGAAAAAACATGCAGTTAGACTTAAAGAAATTGATGCCGAAATAAAAGATATTGAAAAAACAATAAAACAATTAGAAGATAAATGA", "aa": "MDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDK", "aa_hexdigest": "bee78580c5c23544b9149be44aecdab8", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002AC75C79", "query_cov": 0.8858024691358025, "subject_cov": 0.9896551724137931, "identity": 0.634, "score": 347.0, "evalue": 1.51e-116, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [], "truncated": "both", "pseudogene": { "start": 1069491, "stop": 1070840, "observations": { "mutation": [ 1069530, 1069539, 1070535, 1070781 ], "stop": [ 1069530, 1069539, 1070535, 1070781 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KMA1", "query_cov": 1.0, "subject_cov": 0.43724696356275305, "identity": 1.0, "score": 611.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 324, "reference_start": 25, "reference_end": 348, "reference_sequence": "MIFGTLTLKVLKLWYNWFSIGGEIMDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDKWNFEKQRLEELSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSKWTKIPISKLLESDKEKLLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA" }, "paralog": false, "identity": 0.9888641425389755, "score": 847.0, "evalue": 2.71e-309, "description": "Internal stop codon at 1069530, 1069539, 1070535, 1070781. Nonsense mutation around 1069530, 1069539, 1070535, 1070781.", "psc": { "uniref90_id": "UniRef90_A0A3P8KMA1", "product": "ATPase", "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KMA1", "UniRef:UniRef50_I5D648" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "EFPKPENHBP_1956", "locus": "GCA000186005_01953" }, { "type": "cds", "sequence": "contig_1", "start": 1070565, "stop": 1070783, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTAATTTAAAAAGTAAATTAGATGACGCAAGACATAGATTAAATATTTACCAAAAAGAAACAGATTATGAAAAAGCTTCAAAAATTTTGTATGATGAAATTCCAAGCATTAAGAAACAAATCGAAGAAACAGAAAAAGAAATAGCAGAATCAGGTAACACAATTGTTAAAGATAGTGTTACAAGTGAAGAAATTGCTAACATTGTTTCGAAATGA", "aa": "MSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSK", "aa_hexdigest": "44b4d03dc0ad878358a320492855b9e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8257.164299999997, "isoelectric_point": 5.130139732360839 }, "id": "EFPKPENHBP_1957", "locus": "GCA000186005_01954" }, { "type": "cds", "sequence": "contig_1", "start": 1070829, "stop": 1071716, "strand": "+", "frame": 3, "gene": null, "product": "ATPase, AAA family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "nt": "TTGTTAAATCTTGAAAACGATTTAAAGAAAAGAATAAAAGGGCAAGATGCTGCCATTAGATTAGTTGCTCAAGCTGTATTAAGAGCTAAAGCAAATATTAATGATCCTAATAGGCCTTTAGCAAGTTTCTTATTTACAGGACCTACTGGTGTTGGTAAAACAGAATTAGCAAGAGCTCTTGCATATTCTCTTTTTGATAGTGAAAAACAAATGATTCGTTTAGACATGTCTGAATACATGGAAAAACATAGTGTTTCTAAAATCATTGGTGCTCCTCCAGGATATGTTGGATATGACAGCGGTGGATCATTAACTGAAAAAATTAGAAAGAATCCTTACACAATTCTTTTATTCGATGAAATCGAAAAAGCTGACCGCGATGTTCTTAACATTCTTTTACAAATGATGGATAATGGGGCTATTACAGACTCAAAAGGCCGTGTAATTAACTGTCGTAATTTAATTATAATTATGACAAGTAATTTAGGCTCACAAGAAATACTTAAACATTTAGATCAAATGCCAAGTATTAAAGCTGAATTGCTTAAATTCTTGAGTCCTGAATTTGTAAACAGAATTGATGAAATTGTCAAATTTAATCCATTAACTAAAGATGTAATTAATGAAATTGTTGAACTTGAACTTCAAAAATTAGCTACTAGAATGAAAGATGTTAAAGATGTAAAAATATCATTCACAAGCAAAACTGTTGACTTTGTAGGTTCTTCTTCATATGATGAAAACTTTGGGGCAAGGCCTATTAAAAGATTTATCCAAAATAAAATTGAAAGCTTATTAGCTTACAAGATTATTGATGGCACCATAAGAAGCGGAAAAGCCTATAAAATAGACGTTTTCGCCGGAAACTTTGTTGTTAAAGAAGCCTAA", "aa": "MLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA", "aa_hexdigest": "a3df4c8b16e27c37e6fa183f8abd6563", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F4C5962", "query_cov": 1.006779661016949, "subject_cov": 0.8114754098360656, "identity": 0.529, "score": 324.0, "evalue": 4.09e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_F9PSX5" }, "pscc": { "uniref50_id": "UniRef50_F9PSX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "product": "ATPase, AAA family" }, "genes": [], "id": "EFPKPENHBP_1958", "locus": "GCA000186005_01955" }, { "type": "cds", "sequence": "contig_1", "start": 1072014, "stop": 1072199, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATATTAAAGGTGGACATAAAGTTACAGACAGATTGAATCAATTGAATAACATTCCAAAGAATGAAAGAAAAGTTAAAGATGATGACTTAATTCCAGTATTTGAAATAGCTCAAGAACTTTATGCTCGTGGTTTCTATATTGCTAATATTGACTTGAAAAAATCGGCAGCACATGAATGA", "aa": "MIDIKGGHKVTDRLNQLNNIPKNERKVKDDDLIPVFEIAQELYARGFYIANIDLKKSAAHE", "aa_hexdigest": "95c467d4d1a1aa75eca96e0ba2a7235b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7007.9381, "isoelectric_point": 6.723052406311036 }, "id": "EFPKPENHBP_1959", "locus": "GCA000186005_01956" }, { "type": "cds", "sequence": "contig_1", "start": 1072362, "stop": 1072814, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGCTTAAAAAATATACCAATATTAATATTGATGACATTCCAAAAAATGATCCTAAAGTTTTAAGTTTATTTGCAAGTACTAAAGCTATGAATATTAAACCTGAACAAATTAATAATGAAACAACAGGGGCATTAGGTTTGCCTGAATTCGGAACAAGTTTTGTTCGTCAAATGTTAAAAGAAGCTAAACCAAAATCATTTGCTAACCTTATTTCTTTAGCTGGACTTTCTCATGGTGAAAATGTTTGGTTGAACAATGCTCAAGAATTAGTAAGCAAAAAGAATTTTGAAATAACTGATGTTATTTGTTGTCGTGACGACATTATGCAATTTCTTTTAAAGAAAAAAGTTGAACCTTTATTTGCTTTCAATATTATGGAAAAAGTTCGTAAAGGTAAAGGTTTAACTGATGATGAAGTTAAGAGATTAAAAGAATATAAAGTGGCTGACTGA", "aa": "MLKKYTNINIDDIPKNDPKVLSLFASTKAMNIKPEQINNETTGALGLPEFGTSFVRQMLKEAKPKSFANLISLAGLSHGENVWLNNAQELVSKKNFEITDVICCRDDIMQFLLKKKVEPLFAFNIMEKVRKGKGLTDDEVKRLKEYKVAD", "aa_hexdigest": "fbb2e4c24cab980a61775e214946f624", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 149, "aa_cov": 0.88, "hmm_cov": 0.8993288590604027, "evalue": 3.065057104016397e-23, "score": 78.26101684570312, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 17006.64089999999, "isoelectric_point": 9.001349830627444 }, "id": "EFPKPENHBP_1960", "locus": "GCA000186005_01957" }, { "type": "cds", "sequence": "contig_1", "start": 1072902, "stop": 1073363, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07", "PFAM:PF07733.18" ], "nt": "TTGTCAAAAGATGAAAAAATAACAAGTGGATATGACTTACCTAACAAAAATTGCCCTAAATGTAATATTTTATTAGAAGCTGATGGACAAGGAATTCCTTTTGAAACCTTCTTAGGTTTTAAAGCTGATAAAGTTCCTGATATTGACCTTAACTTCTCAGGCGATATTCAAGGTGATATTCACAATGAAGTTAAACGTATTTTTGGTAATACTCACACTTTAAAAGCTGGTACCATATCAACAGTTGCTCTTAAAACTTCATATGGACATGTTAAAGCATTTACAGAAGAAGCAATGCGTAATTATTCAGAACCATTTATTGACTTTTTAGCCCATAAAATAGAAAAATTGAAAAGAACTACAGGCCAACACCCAGGTGGTATTCTTATTATTCCAAAAGAATTTGATGTCTATGATTTTACCCCAATAAACTATCCAGCCAATGAACCTGATTCTTCTTGA", "aa": "MSKDEKITSGYDLPNKNCPKCNILLEADGQGIPFETFLGFKADKVPDIDLNFSGDIQGDIHNEVKRIFGNTHTLKAGTISTVALKTSYGHVKAFTEEAMRNYSEPFIDFLAHKIEKLKRTTGQHPGGILIIPKEFDVYDFTPINYPANEPDSS", "aa_hexdigest": "ccdd409a814e3b155175f57066507a88", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002285EE07", "query_cov": 0.9738562091503268, "subject_cov": 0.8662790697674418, "identity": 0.617, "score": 204.0, "evalue": 1.03e-64, "valid": false, "uniref50_id": "UniRef50_UPI002285EE07" }, "pscc": { "uniref50_id": "UniRef50_UPI002285EE07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 62, "aa_cov": 0.40522875816993464, "hmm_cov": 1.0, "evalue": 2.1676085547131117e-23, "score": 79.51678466796875, "start": 30, "stop": 91 } ], "seq_stats": { "molecular_weight": 17051.052199999984, "isoelectric_point": 5.4928293228149405 }, "id": "EFPKPENHBP_1961", "locus": "GCA000186005_01958" }, { "type": "cds", "sequence": "contig_1", "start": 1073520, "stop": 1073966, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGCTACAGCTGATGTTAAGTATCTAAATTTATATGACAAAGTAGCTTATGAGGTTTTAGTTTATGCTAAAGGAATTAAAAACTCTCGTCATTACTTATTCAACTATGAACAAGCTAAAAACGGAAATATTCCAATTCCAAATCAAGACTATTTAACCACTGAAGAAATGCTTGAACAATTTGCCTTTTTAAAAGACAAACAATTAATTGAAGACATAGTTATAAACAACACAAATAAAATAGCAGACATGTGCGACAAAATTCAAGTTATCAAAGATAAACTTTATACACCTAAGTTTGAAGACAGCGGTAAAAAACTACATGATTTAGTTTATGAAACAGCATACAAAAAATATGGTGAAAAATTACCAAAAATAGTTGAAGAAAGAATTGAAGCCGAATTAAATCCTATTATTGATTATGGTTTTAGTGTTATTTATTGA", "aa": "MVATADVKYLNLYDKVAYEVLVYAKGIKNSRHYLFNYEQAKNGNIPIPNQDYLTTEEMLEQFAFLKDKQLIEDIVINNTNKIADMCDKIQVIKDKLYTPKFEDSGKKLHDLVYETAYKKYGEKLPKIVEERIEAELNPIIDYGFSVIY", "aa_hexdigest": "10c447d9ea582f3c74b17df5b288700d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17316.68769999999, "isoelectric_point": 5.271327781677246 }, "id": "EFPKPENHBP_1962", "locus": "GCA000186005_01959" }, { "type": "cds", "sequence": "contig_1", "start": 1074051, "stop": 1074407, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGAATTAGGTAAAAAAGGTATTTTAACCTTTAATCAACTTAATTCATATATTAAAGATGATCTTTATAATAAAACCTTTTCATATGAAGTTTCAACAATAGCTAAAAACTATGAAGGTTTAAAAGAACAATATGGTTATTTAACAACAGCTTTAACTAAAAACTTATACAATGGACCTAAAACATTTATGGAAGATTTAGCAGCTCATAGTGAAAATCTTTTATTAGGTTCAGGAACACTAAAAAGTAGATTGCTTGATGTTTATTTTTATTCTTCACATGAAGAATTCATTAAAGAATTAAAACTTTATGACTATATTGAAGTGCCAGCACCTCAAGTGTTTAGCCATTGA", "aa": "MDELGKKGILTFNQLNSYIKDDLYNKTFSYEVSTIAKNYEGLKEQYGYLTTALTKNLYNGPKTFMEDLAAHSENLLLGSGTLKSRLLDVYFYSSHEEFIKELKLYDYIEVPAPQVFSH", "aa_hexdigest": "42cf8305374339e3d414d753f2e741cf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13625.23669999999, "isoelectric_point": 5.308500480651855 }, "id": "EFPKPENHBP_1963", "locus": "GCA000186005_01960" }, { "type": "cds", "sequence": "contig_1", "start": 1074417, "stop": 1075136, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGAACACAATGGATGGATTAATTTTTCCTAAAGACATTGTGGCTTTTGCTGAAAAATTGAATCATTCTGCTGTTGCAATTATGGACGTTGATGGTGCTCAAGGTTATCCTGAATTTTATAATGCAGCTAAAAAATCAAAAGTTAAACCTCTTTTTGGAACTGCGTTTTCAGTTATAAACAAAAGTAATAAATCTATTTTAGGAAACATACCTGAAGGCGAAATTAAAGGTCAAGAATATGTTTCATTCGATATTGAAACCACTGGACTTTGTCCTCGTTTTGCCGTAATTATTGAATATGGTTCATTGCCAATTCACTCAAACATGAAGTATGGGAGAAAAACACAATTTTTCATTAAATGTCAAGAACCAATTAAACCTTTTACAGAAAACTTGACAGGTATTACTAATGCAATGATTGAAAAAAGTGGTTTGGAATTAAAAGAAGCTTTGCAAAAAATTTATAATGACTTAAATGGCAAAATTGCTTTAGCACACAATGCTAGATTTGACTTCAACTTCTTAAAAGAAAAATTTAGACAAGCTAAAATAGAATTTCCAAATGTAACAGTAATTGACACACTTATTGTTTCAAGAATTGTTTTCCCTCAAAACAAAAAACATAAACTTGAAGATGTTGCTACTCGTGTTGGTGTTGAATATGATCCTACAGTTGCTCACCGTGGTGATTATGATGCGAAAGTTTTAGCTGAAGTTTGA", "aa": "MNTMDGLIFPKDIVAFAEKLNHSAVAIMDVDGAQGYPEFYNAAKKSKVKPLFGTAFSVINKSNKSILGNIPEGEIKGQEYVSFDIETTGLCPRFAVIIEYGSLPIHSNMKYGRKTQFFIKCQEPIKPFTENLTGITNAMIEKSGLELKEALQKIYNDLNGKIALAHNARFDFNFLKEKFRQAKIEFPNVTVIDTLIVSRIVFPQNKKHKLEDVATRVGVEYDPTVAHRGDYDAKVLAEV", "aa_hexdigest": "f621a5b29f4ee6a7dffb6ca2973db5d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 142, "aa_cov": 0.5857740585774058, "hmm_cov": 0.8169014084507042, "evalue": 4.779158232531776e-06, "score": 22.941926956176758, "start": 83, "stop": 222 }, { "id": "PF00929.31", "name": "RNase_T", "length": 166, "aa_cov": 0.6652719665271967, "hmm_cov": 0.9819277108433735, "evalue": 1.374979097028622e-26, "score": 90.33883666992188, "start": 81, "stop": 239 } ], "seq_stats": { "molecular_weight": 26810.60410000003, "isoelectric_point": 8.349445533752444 }, "id": "EFPKPENHBP_1964", "locus": "GCA000186005_01961" }, { "type": "cds", "sequence": "contig_1", "start": 1075293, "stop": 1075445, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATTCTCAAGACTATGCTTTAAGTAAAAATGGTAAACACATTTACACATTTTTTCTAACCAACTATAAAGATGCTGTAGAAGTAAAAACAATAAGAACAACATTATTAGAACCTGAACAAATAAATGAGCTTGAAGACAACAAATGA", "aa": "MIYSQDYALSKNGKHIYTFFLTNYKDAVEVKTIRTTLLEPEQINELEDNK", "aa_hexdigest": "51ce6b88c245a52c9ac8810f9f25e88d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5926.615999999998, "isoelectric_point": 5.12309169769287 }, "id": "EFPKPENHBP_1965", "locus": "GCA000186005_01962" }, { "type": "cds", "sequence": "contig_1", "start": 1075695, "stop": 1076174, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATAGTAATAATATGCCTAAAGAAAATAGAAAAATAGAAAATGAAAATTTCATTACATTTTGTAAAGATATTAATGTAAAAGTGACACCTGGATTGGATAAAGTTGAATTAGACGATGTCGAATATGACAGTGTTAAAGACCAATTAAATGTGTCATTTAAATTTACAAATTCAATCAGTGCAAAGGAATTTTTTTCGTTTCTTTCTAGTTTAAAAAATAATAAAGTTTATAACATCAAAGATAAAATTACTTTTGATATTTCACAATATAAAAAAGAAAATTTAATAGATTTTATTAACGAATTAACCTATGAAAAAGCCAAATTCATCAAATTGAGAAATTTGCCAATTGTTAAAAAACTTAAATTTAATGATCAATTTGTAGCAACAATTACAACTATTTCAGCTAAAGAATATGAAGAGTTTAACCCCGTTTTTAAAAACCTTCAAAAAACTTTAAATAAATACGGTTGA", "aa": "MYNSNNMPKENRKIENENFITFCKDINVKVTPGLDKVELDDVEYDSVKDQLNVSFKFTNSISAKEFFSFLSSLKNNKVYNIKDKITFDISQYKKENLIDFINELTYEKAKFIKLRNLPIVKKLKFNDQFVATITTISAKEYEEFNPVFKNLQKTLNKYG", "aa_hexdigest": "b473534416b2e5ad896b977cef3f8210", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18743.2437, "isoelectric_point": 8.950290870666507 }, "id": "EFPKPENHBP_1966", "locus": "GCA000186005_01963" }, { "type": "cds", "sequence": "contig_1", "start": 1076361, "stop": 1076495, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD66", "UniRef:UniRef90_A0AB32XD66" ], "nt": "ATGGGAAAACATTTAAATACTGAAAAATGGATAGAAATTTTTATATTTTTTAAAGAATATTTAGACTATAAAATTACAAAGATGGAATTTAAAATGAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAG", "aa": "MGKHLNTEKWIEIFIFFKEYLDYKITKMEFKMNILERATSRQIF", "aa_hexdigest": "64fe2ec0c087f6ace803cb90d8364e4a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1076250, "stop": 1076495, "observations": { "mutation": [ 1076352 ], "stop": [ 1076352 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD66", "query_cov": 1.0, "subject_cov": 0.5432098765432098, "identity": 1.0, "score": 92.8, "evalue": 7.66e-24, "gene_start": 1, "gene_end": 44, "reference_start": 38, "reference_end": 81, "reference_sequence": "MLKINKKNTTNFVVLNQDKRIRLKSQKDLSLFVLWSFMGKHLNTEKWIEIFIFFKEYLDYKITKMEFKMNILERATSRQIF" }, "paralog": false, "identity": 0.9876543209876543, "score": 159.0, "evalue": 1.39e-52, "description": "Internal stop codon at 1076352. Nonsense mutation around 1076352.", "psc": { "uniref90_id": "UniRef90_A0AB32XD66", "uniref50_id": "UniRef50_A0AB32XD66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD66", "UniRef:UniRef50_A0AB32XD66" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD66" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5532.501499999999, "isoelectric_point": 9.157298851013184 }, "id": "EFPKPENHBP_1967", "locus": "GCA000186005_01964" }, { "type": "cds", "sequence": "contig_1", "start": 1076640, "stop": 1076987, "strand": "-", "frame": 2, "gene": null, "product": "MAG0920 family protein", "db_xrefs": [ "RefSeq:WP_245525948.1", "SO:0001217", "UniParc:UPI0010051079", "UniRef:UniRef100_UPI0010051079", "UniRef:UniRef50_UPI00217F505D", "UniRef:UniRef90_UPI00217F505D" ], "nt": "TTGAAATTCAACAATAATTTAAATGACGAAAATCTTTATGAAAAGAAGATTAATTCAGAAACTAACAAAACACAATTTGATTTTTCATTAGGCCAAAAATATCACTATAACTCCCTTCCAAATGAAAAGTCGTTAGTTTCATTCTTTTTCAGATCAGTCGATTTTTATATTAAATATCCAGAAAATTTTGAAAACTTATCTCCTAAAAAGCAAAGAAAATTAGTATATGCAAATTCAATCTTCGATTTTGAAAATACTAAGCCTGATAAATTAACATATTTTGATATTAATATGTTTAAAGACACATTTATTAAATATCAAATTATCCAGAATAATAAAAATGATTAG", "aa": "MKFNNNLNDENLYEKKINSETNKTQFDFSLGQKYHYNSLPNEKSLVSFFFRSVDFYIKYPENFENLSPKKQRKLVYANSIFDFENTKPDKLTYFDINMFKDTFIKYQIIQNNKND", "aa_hexdigest": "08012eba18b2f42d4acc758c829d58d6", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0010051079", "ncbi_nrp_id": "WP_245525948.1", "uniref100_id": "UniRef100_UPI0010051079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051079", "RefSeq:WP_245525948.1", "UniRef:UniRef100_UPI0010051079" ] }, "ips": { "uniref100_id": "UniRef100_UPI0010051079", "uniref90_id": "UniRef90_UPI00217F505D" }, "psc": { "uniref90_id": "UniRef90_UPI00217F505D", "product": "MAG0920 family protein", "uniref50_id": "UniRef50_UPI00217F505D" }, "pscc": { "uniref50_id": "UniRef50_UPI00217F505D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F505D" ], "product": "MAG0920 family protein" }, "genes": [], "id": "EFPKPENHBP_1968", "locus": "GCA000186005_01965" }, { "type": "cds", "sequence": "contig_1", "start": 1077156, "stop": 1077329, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTGTTCATAGATTCAATTTTAGTCAAACAAAATACCTTTAAAAAAATCTTATATTTTTGTCTAACAGTAAGACCTAATTGCATAAGTAATGAAATAAAACTTCAAGATTTTGTTATCAAAAACCAACAAAAATATCAATTATTCTATATGTTAGGTTCATTGGGCTGA", "aa": "MPLFIDSILVKQNTFKKILYFCLTVRPNCISNEIKLQDFVIKNQQKYQLFYMLGSLG", "aa_hexdigest": "7ab5aaceae9cb010f8c936aed4731a07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6747.0185, "isoelectric_point": 9.343483543395994 }, "id": "EFPKPENHBP_1969", "locus": "GCA000186005_01966" }, { "type": "cds", "sequence": "contig_1", "start": 1077398, "stop": 1077688, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5", "UniRef:UniRef90_C4XFQ5" ], "nt": "ATGCTTCCAGATAGTTCTTTAATCGAAAAATCAATTGACATAAACACAGATGTTGAACAATTGTATCCATCTAAAAATAAATGTTATTTTAAAAGACAAGGTTTTAATGCACTTTATTTTTCAACTTTTAATACTCAAAAATTCAAGTCATACTCATTCGAAAAGCAAAAACTTTTTATTTATATGACTATGGTTCTCAATTACGATGATACAGCATTTGATAATAAATTTGAGCCGCTAAATATTAATATGTTCAAAAATGAAGCAAAAGCTTATAAAATTATTGAATAA", "aa": "MLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE", "aa_hexdigest": "9461ed71d51c23faf2888eab3867f672", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1077398, "stop": 1077988, "observations": { "mutation": [ 1077706, 1077727, 1077751 ], "stop": [ 1077706, 1077727, 1077751 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ5", "query_cov": 1.0, "subject_cov": 0.41739130434782606, "identity": 1.0, "score": 194.0, "evalue": 4.1e-61, "gene_start": 1, "gene_end": 96, "reference_start": 135, "reference_end": 230, "reference_sequence": "MSNFLIFSIIFFSFWIAFINLTPFIILFPKIRWYIYAFGSVNKYCMKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPLWYIVVCLLWYIKQKKWRKFNNMLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE" }, "paralog": false, "identity": 0.9846938775510204, "score": 383.0, "evalue": 5.2e-138, "description": "Internal stop codon at 1077706, 1077727, 1077751. Nonsense mutation around 1077706, 1077727, 1077751.", "psc": { "uniref90_id": "UniRef90_C4XFQ5", "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ5", "UniRef:UniRef50_C4XFQ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11489.03199999999, "isoelectric_point": 7.744960594177246 }, "id": "EFPKPENHBP_1970", "locus": "GCA000186005_01967" }, { "type": "cds", "sequence": "contig_1", "start": 1077749, "stop": 1077955, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAATTAAGAGTTCAAAATTCATTAGTAAATCATTATTGTCTGATGTCTAAAATTCCATTAATTTATGCTTTACTTGCAATCGCATTAATGCTAGAAAGCTTAGTTTTAATGGTTTTTCTACAAGTATATGAAAAGGAATCATTTTTTACAATGTTTGTAACACTTGGCATAATATTTTATGTACCATTGCCCTTGTGA", "aa": "MKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPL", "aa_hexdigest": "73034ecf5c94e4b169ab768fbfd9b822", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.764499999995, "isoelectric_point": 9.301514625549316 }, "id": "EFPKPENHBP_1971", "locus": "GCA000186005_01968" }, { "type": "cds", "sequence": "contig_1", "start": 1078017, "stop": 1078292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGCGTAAGATTAATAAAAGCAATTCAAAAACTAAAAAAGATAATTGAAAAAATTAAAAAATTACTCATATGTGATTATCCTAAATTATCATATAAAGAATTATTAGCTTTTTTACGCAAATATGTTCCTTTTTTAGGGTCATTAACTCTTTGTAAACTTCACAAACTTAATTCAAAATCAGAAAGCTCTAGCATTTCATTAATAGTTTTTATTTCGTTGGTTTCTAAATTTTTAAAAATAAGGTTTTTTCCACCAGTACCTCCCATATAA", "aa": "MNGVRLIKAIQKLKKIIEKIKKLLICDYPKLSYKELLAFLRKYVPFLGSLTLCKLHKLNSKSESSSISLIVFISLVSKFLKIRFFPPVPPI", "aa_hexdigest": "5f1d287fa27a157290594ec3daa0662e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10476.840899999988, "isoelectric_point": 10.203556251525882 }, "id": "EFPKPENHBP_1972", "locus": "GCA000186005_01969" }, { "type": "cds", "sequence": "contig_1", "start": 1078328, "stop": 1078582, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCAAGTATTCCTATGGCATTAAATCGTGTTGCTAGAGTATATGAAATGCTTAAATTATCACAAGCTGCGCCATCAATAGCAACTATGGCTACTGGTGTTGTTGGTTCAGCATTACAAACTCTTGGATCATTTTATGATGTTTACACCGCTTCAAAAGAATTAGATTATTTTAATGAATGTGCTAAAAAGGCATCAGATTTAATTAGTGATTTTTCAAAGAAAATAAAAGATATTAAAAAAATAGGATGA", "aa": "MVSSIPMALNRVARVYEMLKLSQAAPSIATMATGVVGSALQTLGSFYDVYTASKELDYFNECAKKASDLISDFSKKIKDIKKIG", "aa_hexdigest": "739759822f8d92f287065c50fef326ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9127.559099999993, "isoelectric_point": 9.09850368499756 }, "id": "EFPKPENHBP_1973", "locus": "GCA000186005_01970" }, { "type": "cds", "sequence": "contig_1", "start": 1078958, "stop": 1079407, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTATCTACTTTACTAAATACAATTAATGTAACTAACATAACACCATCAGTCATTGTTGCATCAAATGAAAATTTAAATTCAGAGCAACAAAAAATTTTGGAATTATTTTCTCGATTTACAAACGATGAAATTGAAATTTTTAACTATATCTCAAATGATATTATCAATAACAAAAATTCAACTAAAAAAGTTATAAATTCCATTGATGAAATAAAATTAGATAAAATTTATGATTTGTTTATATCTAAAATTAGCCAATCTAGTATAAAAAATAAAGATGAATTATTAAATAAACTAAGAGACAAAAGAGAAATTTTTAAAACTGAATTAATTAAAAAAATAAAAGAACAATCTTTAACTTTTAAAAAAAGCGAAAAAAAATCAAAAATAGAAATCAATTCAATGATAAAAAGAGTCAATTCGTCTTTTGAAAATAAAGATTGA", "aa": "MKLSTLLNTINVTNITPSVIVASNENLNSEQQKILELFSRFTNDEIEIFNYISNDIINNKNSTKKVINSIDEIKLDKIYDLFISKISQSSIKNKDELLNKLRDKREIFKTELIKKIKEQSLTFKKSEKKSKIEINSMIKRVNSSFENKD", "aa_hexdigest": "f4afcd0e1f87f7841e4c727d1bb7c2e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17375.80749999999, "isoelectric_point": 9.397314643859861 }, "id": "EFPKPENHBP_1974", "locus": "GCA000186005_01971" }, { "type": "cds", "sequence": "contig_1", "start": 1079863, "stop": 1080204, "strand": "-", "frame": 1, "gene": "exiS", "product": "ExiS", "db_xrefs": [ "RefSeq:WP_013355043.1", "SO:0001217", "UniParc:UPI0001E3307C", "UniRef:UniRef100_A0AB32XDB6", "UniRef:UniRef50_A0AB32XDB6", "UniRef:UniRef90_A0AB32XDB6" ], "nt": "ATGTGTAAAGTTTCAGATTACAATGTAGCAAAACAGTTAATATTGTTTTTTATTTTACAATTATTTTCAATGTTAAAAGTTCCTACCTATAAAGATTTAAATATAAATATTAAAAAGATTTTGTGGACTAAAAATTATAAAAGCTTTACAGAGTTCCAAAAGGCATATAGTGGCGTTTTTTGTGCTAAATTAGGTCATTATCTAAGTAAGCATAAAGCCAACACCAAAACAAATTTTTTAAATATGTATCAAATTATTTTTTATCAAGGTTTTATTAATTACAACAGAGAAAAGGAATTATTAGATTTATTGACTTATAAAGAAAAGTCTCAGACATATTAA", "aa": "MCKVSDYNVAKQLILFFILQLFSMLKVPTYKDLNINIKKILWTKNYKSFTEFQKAYSGVFCAKLGHYLSKHKANTKTNFLNMYQIIFYQGFINYNREKELLDLLTYKEKSQTY", "aa_hexdigest": "ca41be5796e33309c75e728d0372d953", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3307C", "ncbi_nrp_id": "WP_013355043.1", "uniref100_id": "UniRef100_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307C", "RefSeq:WP_013355043.1", "UniRef:UniRef100_A0AB32XDB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDB6", "uniref90_id": "UniRef90_A0AB32XDB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDB6", "product": "ExiS", "uniref50_id": "UniRef50_A0AB32XDB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDB6" ], "product": "ExiS" }, "genes": [ "exiS" ], "id": "EFPKPENHBP_1975", "locus": "GCA000186005_01972" }, { "type": "cds", "sequence": "contig_1", "start": 1080922, "stop": 1081242, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAATAATATCATCAAAATTAGTTTTTTTTCTTAAAAAATCTTCTAACTAA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSIISSKLVFFLKKSSN", "aa_hexdigest": "920815c94b6ebd0c1000b79640b9735d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12364.57399999998, "isoelectric_point": 9.96186428070068 }, "id": "EFPKPENHBP_1976", "locus": "GCA000186005_01973" }, { "type": "cds", "sequence": "contig_1", "start": 1081498, "stop": 1081770, "strand": "-", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_259637433.1", "SO:0001217", "UniParc:UPI000FF5E58A", "UniRef:UniRef100_C4XFQ1", "UniRef:UniRef50_C4XFQ1", "UniRef:UniRef90_C4XFQ1" ], "nt": "ATGACAAGTACTATTTCGCAATTTGCTTCACAATACCAAATGAGTAATTTACAAAAGTATCGTAGTGTAGAAACAATAATTAATATGGAAAAAGCCATATTCTTGAAAAATCGTCAAGAAGATTTTATAGAAAAATATTACAAGAAGAATGAAAATTCAAGATCTAATAATTACTTTTCAGACAAATTCAAAAGAGTATACAATTGCTTAAGCGGTGTAAGTCAACTGATCATTAAAAATGAGTTTATGAAACAATCTGAAAACAAGTTATGA", "aa": "MTSTISQFASQYQMSNLQKYRSVETIINMEKAIFLKNRQEDFIEKYYKKNENSRSNNYFSDKFKRVYNCLSGVSQLIIKNEFMKQSENKL", "aa_hexdigest": "de9335703414c11ffa78d0d92e3af720", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000FF5E58A", "ncbi_nrp_id": "WP_259637433.1", "uniref100_id": "UniRef100_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FF5E58A", "RefSeq:WP_259637433.1", "UniRef:UniRef100_C4XFQ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFQ1", "uniref90_id": "UniRef90_C4XFQ1" }, "psc": { "uniref90_id": "UniRef90_C4XFQ1", "product": "Transposase", "uniref50_id": "UniRef50_C4XFQ1" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ1" ], "product": "Transposase" }, "genes": [], "id": "EFPKPENHBP_1977", "locus": "GCA000186005_01974" }, { "type": "cds", "sequence": "contig_1", "start": 1081812, "stop": 1082717, "strand": "-", "frame": 2, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_Q98R27", "UniRef:UniRef90_C4XFQ0" ], "nt": "ATGATTTCAACTTATTCAAAATTTTTAAAAAACTTTTTAGCAATACCAACAATTATTGGTAAGAAAACGCCAAAAGAAAAATTTGCTGGCGCATGTTCAACTTATACAGTTGAAGCAATGATGAAGGATGGTCGTGCTTTACAAGCAGGAACAAGCCATTATTTAGCTCAAAACTTTTCAAAACAATTTGAAATTCAATTCAAAACTGAAAACAATGAATTAGATTATGCTTACCAAACTTCATGGGGTGTTTCAACCCGTCTGCTTGGAGCTATTATAATGACTCATGGAGACAATAGAGGAATTATTATTCCACCTCGAGTAGCTCCAATTCAAATAGATGTTTTAGAGCTTTTTGCAAATAAAAATAAAGAAGTTCATACATACTGTGATAATCTTTACAAAGACCTAAGTAGAAAATTTAGAGTAAGACTTGATTCAAGTGACAAAGGTCCAGGATTTAAAGCCTCAAATTCTGAAATTCAAGGTGTTCCTCTTAGAATTGAAGTTGGACCTAAAGACATTGAAAATAAAAGTGTGACAATTGTTAGAAGAGACACCCTTGAAAAGATTAATATAAAACAATCAGAAGTTAAGGATCAAGTTGAAACTCTTTTAAATCAAATCCACGATAACTTGTACTTACAAGCTGAAAAAAGACTAAATGAAAATACAGTCTTTGTAGATAACTACGAAGATTTCAAAAAACAAATTAAAAATCATAAATTTGTTATAGCCCCATTTTGTTGTTTAGATGTGGCTGAAGAAATAATCAAAAATGAAACTGGCGCTTCAACTCGTTGCTTACCTAAAAAGTTTGATAAACCAAATCAAAAACACAAATGTATTTTTGAAAAATGTTGTAAAGAAACCAATAGATATGTAATCTTTGCAAGAGCTTACTAG", "aa": "MISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY", "aa_hexdigest": "2e3c870226400edc3a969c193508d9d3", "start_type": "ATG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "3-prime", "pseudogene": { "start": 1081812, "stop": 1083017, "observations": { "mutation": [ 1082777, 1082822, 1082840 ], "stop": [ 1082777, 1082822, 1082840 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ0", "query_cov": 1.0, "subject_cov": 0.6244813278008299, "identity": 1.0, "score": 604.0, "evalue": 1.44e-215, "gene_start": 1, "gene_end": 301, "reference_start": 182, "reference_end": 482, "reference_sequence": "MIKIMKELEKITPLEQDFAKWYTDVVKQGNLIAYGPVKGTIVFKPNSYGIWEQIQKNLNECFREKGVQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQWANVVRWEKTTNPFLRSREFLWQEGHTCHSNPLEARKFTKGMISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY" }, "paralog": false, "identity": 0.9925187032418953, "score": 801.0, "evalue": 7.51e-296, "description": "Internal stop codon at 1082777, 1082822, 1082840. Nonsense mutation around 1082777, 1082822, 1082840.", "psc": { "uniref90_id": "UniRef90_C4XFQ0", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q98R27", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ0", "EC:6.1.1.15", "UniRef:UniRef50_Q98R27", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R27" ], "product": "Proline--tRNA ligase" } }, "id": "EFPKPENHBP_1978", "locus": "GCA000186005_01975" }, { "type": "cds", "sequence": "contig_1", "start": 1082838, "stop": 1083062, "strand": "-", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "GTGCAAAATGTATATTTGCCACTTTTAATACCTGAAAAATTGTTTAACAAAGAAAAAGATCACATAGCAGGATTTAATCCAGAATTAGCAACAATTACTAAAGTTGGCGACAAGGATTTAGATGAAAAAGTATTTATTAGACCAACATCAGAAGTTTTATTTGCTGATTTATTTAAAAACTCAATTGAGTCTTACAAAGACTTACCTATGATTTATAACCAATGA", "aa": "MQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQ", "aa_hexdigest": "22489394e1143e09c5d48d3cbf0b2e0b", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 0.891566265060241, "identity": 0.527, "score": 87.8, "evalue": 2.74e-21, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_1979", "locus": "GCA000186005_01976" }, { "type": "cds", "sequence": "contig_1", "start": 1083380, "stop": 1083532, "strand": "-", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013355046.1", "SO:0001217", "UniParc:UPI0001E3307F", "UniRef:UniRef100_C4XFP9", "UniRef:UniRef50_P35871", "UniRef:UniRef90_C4XFP9" ], "nt": "ATGCCAAGAGACGGTTTTACATTAGTTTGCGAATCATGTAAAATGGAAAACTACATTAGTAAAAAGAACAAGAAAAATACACCAGAAAAAGTTGAATTAAGCAAATATTGTTCAAAATGTAGAAAACACCAAAATCACAAAGAAAAGAAATAA", "aa": "MPRDGFTLVCESCKMENYISKKNKKNTPEKVELSKYCSKCRKHQNHKEKK", "aa_hexdigest": "4965179e4d6cbe3993b3906007accba1", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3307F", "ncbi_nrp_id": "WP_013355046.1", "uniref100_id": "UniRef100_C4XFP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307F", "RefSeq:WP_013355046.1", "UniRef:UniRef100_C4XFP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFP9", "uniref90_id": "UniRef90_C4XFP9" }, "psc": { "uniref90_id": "UniRef90_C4XFP9", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "EFPKPENHBP_1980", "locus": "GCA000186005_01977" }, { "type": "cds", "sequence": "contig_1", "start": 1083909, "stop": 1084088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGACAATAATGAATATTTGACTGATGGAGAGTTAGCTTTATCAAATAATGAACCAATACGAAGTCAATGTCGTAGTCCATATTCATATTTTACTAAACCAAAAAGAAGAATAGTTTGCGAAGGATATGCAAGAACATTTTCAAGATTTCTAACATTAATAAATATTCCTGTGTGA", "aa": "MIDNNEYLTDGELALSNNEPIRSQCRSPYSYFTKPKRRIVCEGYARTFSRFLTLINIPV", "aa_hexdigest": "e4f011fdaf9d1c7e48010e50eee049db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6902.802399999999, "isoelectric_point": 8.736062431335448 }, "id": "EFPKPENHBP_1981", "locus": "GCA000186005_01978" }, { "type": "cds", "sequence": "contig_1", "start": 1084326, "stop": 1084826, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTAAAGAAAGCTTTAAAACTTAAAAAAGAATTATTAGAATTAAATGAAAAAATTTTTAATGCTTTTGGATTACATGAGGGAAGATTTGAAAGTTATTTAAATGCTTACAAAAATTTCGTTAATATAGGTTTTGTACAATCAAATACAGGATTAACTGCTACAATAAATGGGAATCCTGAACCTACAATAAAATATATTTTAGAGAATTATATATCTCAAGGAAGTCTTCTTAATCTTAATTATAAATTTTTTAACGACATTTTAAGCAATATAGAAAAATCTGTAAAGTACTATAAAAAAGACTTAGACTTATCATTTGACATTAAAGATGCATATGAAGTTGATTTAAACAATTTGGACTTTCTAAAAAGTGCTAAAGCTGTTGATACTCTCACAAAAGAACAAGTTTTAAAAATTAGAAAAGACTTGCTATTTAAAGGAAAAATAACTTCTGATATTGCCGTAACTAATCATAATTTAGGAGATTATTTCTGA", "aa": "MLVKKALKLKKELLELNEKIFNAFGLHEGRFESYLNAYKNFVNIGFVQSNTGLTATINGNPEPTIKYILENYISQGSLLNLNYKFFNDILSNIEKSVKYYKKDLDLSFDIKDAYEVDLNNLDFLKSAKAVDTLTKEQVLKIRKDLLFKGKITSDIAVTNHNLGDYF", "aa_hexdigest": "8a39ac61662c67e5222f7cc6ab116429", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19074.695900000002, "isoelectric_point": 8.552198600769042 }, "id": "EFPKPENHBP_1982", "locus": "GCA000186005_01979" }, { "type": "cds", "sequence": "contig_1", "start": 1084966, "stop": 1085184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGATGGTTTATTATCTCCAGGTTTTGGGGCTGGGCCTGGACCTGGTTCTGGTGTTACAGGTGTAACTGTCGTACTTGGTGTTGAAGGTATATTGCCAGTAACATCATCTCAAAAACATGATGTTGTTATAGTTGGTAGACTAACAATAGCAGTTAGAGTACCTAGTCAATTAAATATTTTATTTTTTTTCATATTTATCTCTTTCTACAATTAA", "aa": "MLDGLLSPGFGAGPGPGSGVTGVTVVLGVEGILPVTSSQKHDVVIVGRLTIAVRVPSQLNILFFFIFISFYN", "aa_hexdigest": "7b7adee789e7d8425142ec843d51200e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7473.685599999998, "isoelectric_point": 6.509280967712402 }, "id": "EFPKPENHBP_1983", "locus": "GCA000186005_01980" }, { "type": "cds", "sequence": "contig_1", "start": 1085779, "stop": 1086273, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGAAACTTTATTGATAGAAACAAAAAGGATCTTGAATTAGTTTTTGATTTAAAAGATGCTAAACAAATTAATATTAATAATTTAAAAATGTATACAGAAAATATTAACTCAGTTTTAAACAAACAATTGAATGTTGAACATTTTAGAAAATCATTAGTTAAAAATGGTGTTAATGTTGCTCCTCTTATCAAATATTATTATGGCACGGATAAATATAACAGCTTAAGAAATTTAGTTTATGATCTAACGGGCGGTTCAAACTATTTTAATGATTTTTGTTTTAAAGGAAACCACTATAAAAATTCAACATCTGTTACATTTTATCAACCAGAAAATAATGTAATAAACATTAAAATAAATCGTTTAGCTTATAGAACTGAAAATGCACAAGAAGAAATTGATATCAATAAATATTTTGAAAAAATTATTTATTCTATTATTTCAAAACAAATGAATGATGAACAAAAAATTAGAGCTATTCACAATTGA", "aa": "MFRNFIDRNKKDLELVFDLKDAKQININNLKMYTENINSVLNKQLNVEHFRKSLVKNGVNVAPLIKYYYGTDKYNSLRNLVYDLTGGSNYFNDFCFKGNHYKNSTSVTFYQPENNVINIKINRLAYRTENAQEEIDINKYFEKIIYSIISKQMNDEQKIRAIHN", "aa_hexdigest": "54a25e6fd275c82fd7a801c307af3c49", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19498.898500000003, "isoelectric_point": 9.24742565155029 }, "id": "EFPKPENHBP_1984", "locus": "GCA000186005_01981" }, { "type": "cds", "sequence": "contig_1", "start": 1086358, "stop": 1086525, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAACAATGATGTTTATGATAAATTTGGTTTGCATGAAAACCGTTATAAAAAATATTTAGTTGCTTACAAAAATTTTATAAATTTATATATTGATAATGAAAATAGCAGAGCTTATATTTTTGATCTTTTAGCTCCAAAACATAATAATTTTTGTTCCTGA", "aa": "MQLNNDVYDKFGLHENRYKKYLVAYKNFINLYIDNENSRAYIFDLLAPKHNNFCS", "aa_hexdigest": "48f75f87877629d512ef708cbdd1566c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6679.4655, "isoelectric_point": 7.863096046447754 }, "id": "EFPKPENHBP_1985", "locus": "GCA000186005_01982" }, { "type": "cds", "sequence": "contig_1", "start": 1087182, "stop": 1087331, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAATGATTGACTCAGGAGAAACAGATACAAAATTAATTGCTGTTCATGCTGATGATTATCGTTTAGATGGTATTAAAAAATTAGAAGACTTACCTGAACCATTTTTAATGCACATTGAAACATTCTTTAGCAACTATAAAAATTGA", "aa": "MKMIDSGETDTKLIAVHADDYRLDGIKKLEDLPEPFLMHIETFFSNYKN", "aa_hexdigest": "8655a983f95376eb1fa7e1c087a748f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 49, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.215019112941612e-15, "score": 51.09521484375, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5731.486999999998, "isoelectric_point": 4.8155927658081055 }, "id": "EFPKPENHBP_1986", "locus": "GCA000186005_01983" }, { "type": "cds", "sequence": "contig_1", "start": 1087416, "stop": 1087574, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAGTAATATTATTGAAATGAAAGTTGAAATTCAAAAGAATTCAAACATAAAATACGAATATGATCGTGCTGACGGAAATATTCATGTAGACCGTATTTTGAGAGGAGATTTTGTCTACCCATGTAATTATGGCTTTATTCCTACAGCTTTAGATTGA", "aa": "MSNIIEMKVEIQKNSNIKYEYDRADGNIHVDRILRGDFVYPCNYGFIPTALD", "aa_hexdigest": "09b23b3bc4c5ae2db9338f44658ea512", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 2.6817974698508896e-14, "score": 49.5887451171875, "start": 8, "stop": 51 } ], "seq_stats": { "molecular_weight": 6080.8351, "isoelectric_point": 5.1192834854125975 }, "id": "EFPKPENHBP_1987", "locus": "GCA000186005_01984" }, { "type": "cds", "sequence": "contig_1", "start": 1087727, "stop": 1087987, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4", "UniRef:UniRef90_C4XFP4" ], "nt": "ATGATTTTAAACTCAGTATTTTATAAAGCAAATAACCCATTTTACGAACAAGGAACACACAAATTAAATGCTCCTTATTTAGCTTTATTTATTATTGGATTATCACTAATAGTAATTGGAATTACATGCTTTTTCTTTTATCCTAAAGCAAAAGACAAGGTTTATTTGTATAAAGAAAAACAAATGGAAGAATATAAAAAAAATAATCCAAAATCTAAAGTAACGAATTATGAAGCAACAGGAATGTATTTACCAGCTTGA", "aa": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPA", "aa_hexdigest": "2af4347964a77378f824706534822d7e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1087631, "stop": 1087987, "observations": { "mutation": [ 1087729 ], "stop": [ 1087729 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP4", "query_cov": 1.0, "subject_cov": 0.7288135593220338, "identity": 1.0, "score": 176.0, "evalue": 1.57e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPAWERIKLFAPLFFGILFVVVGITMIVGKTISTL" }, "paralog": false, "identity": 0.9915254237288136, "score": 236.0, "evalue": 1.26e-81, "description": "Internal stop codon at 1087729. Nonsense mutation around 1087729.", "psc": { "uniref90_id": "UniRef90_C4XFP4", "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP4", "UniRef:UniRef50_C4XFP4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10015.642899999993, "isoelectric_point": 9.301836967468262 }, "id": "EFPKPENHBP_1988", "locus": "GCA000186005_01985" }, { "type": "cds", "sequence": "contig_1", "start": 1088100, "stop": 1088330, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGACTAAAGAATTAAAAAAACCTAAAATAATTTTTATCGATTTGGATGGTACAACAATTGATAAAAGTAGCAAATTTAAAAAAGATATCAGTGAAATTAACCAAAAAGGTTTAGCTAAACTTAAAGAAAAATATGGCTTAGAAATTTTTGTTTCAACTGGCCGCGCAGTAGTTGAAAAAACAAAAATGTTGGCTAGAAAATGTGGCAATGAAAATAATTTTGTTGCTTGA", "aa": "MTKELKKPKIIFIDLDGTTIDKSSKFKKDISEINQKGLAKLKEKYGLEIFVSTGRAVVEKTKMLARKCGNENNFVA", "aa_hexdigest": "e200c3365709a433716dadbf93c62d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 55, "aa_cov": 0.7236842105263158, "hmm_cov": 0.9090909090909091, "evalue": 1.5756191560247932e-07, "score": 27.63774871826172, "start": 11, "stop": 65 } ], "seq_stats": { "molecular_weight": 8578.032499999998, "isoelectric_point": 9.678396797180177 }, "id": "EFPKPENHBP_1989", "locus": "GCA000186005_01986" }, { "type": "cds", "sequence": "contig_1", "start": 1088430, "stop": 1088630, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTATTATTAACTCAAACTTTAAAAATGGTTTGTACTCGAATTCATTTCTTTTTAAAGTTGCTTCAATTTTCAAAAAAGGCAAATGCTACAAACTAAAAGAATTTAATAAAGAAACTGAAGTATTTAAGTTACTTTTTCTTAATTTGTCAAAGAAAAAACTTCATTTATTTGCTGAAGAACTTAAAAAATTATGA", "aa": "MTIIINSNFKNGLYSNSFLFKVASIFKKGKCYKLKEFNKETEVFKLLFLNLSKKKLHLFAEELKKL", "aa_hexdigest": "04644b2c10858d67879e920fa9cb07b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7807.306799999996, "isoelectric_point": 9.903262519836424 }, "id": "EFPKPENHBP_1990", "locus": "GCA000186005_01987" }, { "type": "cds", "sequence": "contig_1", "start": 1088805, "stop": 1088936, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGTAATTGCTATGAAAAACTCTGTTCCTGAATTTAAAGATAAAGCAGATTTTATTAGTCCTTTTACAAATAAAAAAGGTGGTTTATATAAAACTTTAGAAATGTTACTTACAAAGTTTGAATAA", "aa": "MGYVIAMKNSVPEFKDKADFISPFTNKKGGLYKTLEMLLTKFE", "aa_hexdigest": "eff5f802b51b4971c1ac375fb2db53a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4931.786999999999, "isoelectric_point": 9.100824546813964 }, "id": "EFPKPENHBP_1991", "locus": "GCA000186005_01988" }, { "type": "cds", "sequence": "contig_1", "start": 1089163, "stop": 1089375, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTATTGTTGCCACTTTTGTGTGTCAAACTGTATTTTATAAATACAAAAGAGAAGGTGTGGATTACATTTTATATTCAAAACCTTTAAGTAGAACTCAAATATATTTTGCTAATATTTTTGTATCTGCAATTGGTCTATTATTTTCAATAGCAATTATCGAATTAGGTTATTTTATAAGCAGATTGGTTTATCCAACAATTATTTGA", "aa": "MIFIVATFVCQTVFYKYKREGVDYILYSKPLSRTQIYFANIFVSAIGLLFSIAIIELGYFISRLVYPTII", "aa_hexdigest": "2e51f333131899ab4915be886a604b38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8142.654399999993, "isoelectric_point": 9.20616588592529 }, "id": "EFPKPENHBP_1992", "locus": "GCA000186005_01989" }, { "type": "cds", "sequence": "contig_1", "start": 1089607, "stop": 1089801, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTAAAGAAAAAGGCAACAAGAAATTCCTTGAAGAATTAAAAGGCAGATTCAATGAAAATCAACCTATCTTGATGAGTAGATACAACGATGACTTAAAAAATACTTTATTAAATATTACAGAAGATTCAGAAGATATCACAAAATATATTGACAATACTCATAAGTCAGTTTATAACTATTTATATTGA", "aa": "MPSKEKGNKKFLEELKGRFNENQPILMSRYNDDLKNTLLNITEDSEDITKYIDNTHKSVYNYLY", "aa_hexdigest": "5f242073680cef39c575dfd506138f65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7661.503400000002, "isoelectric_point": 5.727744865417481 }, "id": "EFPKPENHBP_1993", "locus": "GCA000186005_01990" }, { "type": "cds", "sequence": "contig_1", "start": 1090159, "stop": 1090398, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAGTTAAAACTTTTTGAATCAATATTTTTAGATAAATTATATGAAAATATTCAAGAGATCAAATTTAACAAATTACCTTATGTTATAGATTACGAGGGCAAAAAGGTATTAGTCTTATCAAAAGTATTTCAAGACTTTAACAAAATATTAAGCTATGTTGAAGAACATCAAAAAGATAAATACAAAAACTTAAAAATTAAAACGTATATTGCAACATATTATCCACAATGA", "aa": "MIDKLKLFESIFLDKLYENIQEIKFNKLPYVIDYEGKKVLVLSKVFQDFNKILSYVEEHQKDKYKNLKIKTYIATYYPQ", "aa_hexdigest": "5d26c0ca6e516fa7d0391693782ee8a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9646.199099999994, "isoelectric_point": 9.002639198303225 }, "id": "EFPKPENHBP_1994", "locus": "GCA000186005_01991" }, { "type": "cds", "sequence": "contig_1", "start": 1090603, "stop": 1090833, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCTTATGTATGGTTTAAATAAAGAACAATATGTTTTAAGTTATCAAGCTTCACCTAATAATTTGGATGAATTAAATGAATTAGACAATGCAGGTGGTATTGAATTAATTAAATTAACACCACTTGAAAGAGATTTAATTGTTGTTACAAGAAAAGATCACATGACAGTTGGCGGATCAATAATTTTATCAATGTTAGCTGCTTTTGCTATGATGGCTGGTGGTTGA", "aa": "MTLMYGLNKEQYVLSYQASPNNLDELNELDNAGGIELIKLTPLERDLIVVTRKDHMTVGGSIILSMLAAFAMMAGG", "aa_hexdigest": "3da7af128e9fc4b9501d01fd990d5ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8301.588199999998, "isoelectric_point": 4.597785758972168 }, "id": "EFPKPENHBP_1995", "locus": "GCA000186005_01992" }, { "type": "cds", "sequence": "contig_1", "start": 1091289, "stop": 1091753, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1", "UniRef:UniRef90_C4XFP1" ], "nt": "ATGTCAAATTTAAAACCTGAAGAAGCAAAAGAAAAAGTAAATTTCTTATTAAAAAAATATCACTTTGATGGACCTGAATTTGAAAAATCAGCAGAGCAACTTTCATCAGGCCAAAAGAAAACAATTTTATTAATGCAAGCTTTATTAAATGATCCTGAAATTTTAATTATGGATGAACCTGCTGCTAATTTAGACCCAAGTGCTAGAGTGATATTTTATGAACAAATAAAAGAATTAAGTTTACAAGGAAAAACTATATTTATTTCTAGTCATATTCTTCTAGAACTTGAAAGATATATCGATTCTTTTACGGTGCTTGCTAAGGGCAAAGTACTAGACAATGGAACAATAAAAGAAAAATTGCACAATGCAAAATATAACAAAAAAATAAATACAAATGATAACAATAAAATAGTGGAATATTTAATTAAAAACCGAATTGATTTTATTGAAAAACATGATTGA", "aa": "MSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHD", "aa_hexdigest": "ce46563bf145cbb5fce075b9339ef461", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1090989, "stop": 1091891, "observations": { "mutation": [ 1091751 ], "stop": [ 1091751 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP1", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 295.0, "evalue": 2.66e-98, "gene_start": 1, "gene_end": 154, "reference_start": 144, "reference_end": 297, "reference_sequence": "MMKTLVKKSKWRIYLEKRANYKATIKRAKNYEYQQPQEDVAISIQHLHKFFKLGHGDFKTALDDVCFDVKKGSFHGFIGDNGAGKTTTIRSILGFYPSKIGKIFINGIDSTNKKAKSFIGYIPEVSLFPRKVKVKEYLLAFAEMSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHDWINAKLDEEQVNKFLQYCLKNNIQIYNLADNKLSLNELYFNAENVE" }, "paralog": false, "identity": 0.9933333333333333, "score": 557.0, "evalue": 4.58e-204, "description": "Internal stop codon at 1091751. Nonsense mutation around 1091751.", "psc": { "uniref90_id": "UniRef90_C4XFP1", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP1", "UniRef:UniRef50_C4XFP1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1" ], "product": "ABC transporter domain-containing protein" } }, "id": "EFPKPENHBP_1996", "locus": "GCA000186005_01993" }, { "type": "cds", "sequence": "contig_1", "start": 1091949, "stop": 1092722, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTCACACAAGATGAATACAACAAAGTTGAATCTAATAAAAATATCATTAATTTTGAAAAAGCAAGAGAAGAAATTACTGAGAAATATCAAATTGAACATCTTGAATTAATTGAGCAATTATTCAAAGAATCATACTTAGGTTTAGACTGTCAATATTACATGAATGATAGTCATCAACTTTCAAGTGAAATCAAATTAGATAATTCAGATGATGATGAAAATGGTGGCGAAAAAGAAGTTGACTTAAATGTCGAAGTTCAAAATGTTGATAATCTTGAACAATTACAAGCAGAAGCTCAAAAAGTTGTTGATTTAGAAAACTTGGCTAACTTTGAAGAAGCTGAAATTGAAGAAAAGAAAAATCAAAAGGTTGAAACTCAAAAGATAGAAGAAGATGCAAAAGAAACAATTACAGAAATTAAGGAAGAAGTTGTTCCTGAAACTAAAGTTGAAGAACAAAATATTAAAGTTGAAGAAGTTAAAAATCCTTCTGCACAACAAGCTCAACCAAATCAACCTAAATTCAATTTAAATAAACCTCAAGATCAATACCAACAAACTTCTAGACTTCGCCAAGTAAATCCAAATATGTATCAAAATGTTCAATATCCAAACCAATATCAAAACTTTCCAAATAAAGGCTATGTTGATCCAAGATTTGCACAATATCCTCCTAGACAAAACCCATATTATCCAAACCAAAATTATACAAACATAAATGTAGCTCAAAGACAACCACAAAATCCTAATAATCCTATAAAACAATAG", "aa": "MDFTQDEYNKVESNKNIINFEKAREEITEKYQIEHLELIEQLFKESYLGLDCQYYMNDSHQLSSEIKLDNSDDDENGGEKEVDLNVEVQNVDNLEQLQAEAQKVVDLENLANFEEAEIEEKKNQKVETQKIEEDAKETITEIKEEVVPETKVEEQNIKVEEVKNPSAQQAQPNQPKFNLNKPQDQYQQTSRLRQVNPNMYQNVQYPNQYQNFPNKGYVDPRFAQYPPRQNPYYPNQNYTNINVAQRQPQNPNNPIKQ", "aa_hexdigest": "1458df4c6c86c3b26ebe3a48c7d19f9c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30352.7891, "isoelectric_point": 4.50729808807373 }, "id": "EFPKPENHBP_1997", "locus": "GCA000186005_01994" }, { "type": "cds", "sequence": "contig_1", "start": 1092807, "stop": 1092953, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATGAAAGCAAAGAAAAAGTATTTGCCAACATTAAATTAGATGGTAAAGCAATTACAGTTAAATCACTTGCATATTTATTAACCTTTAATTTATGTTCACAATTAAACTTAGATATGAATTTAACTGAATCGTTCTACTGA", "aa": "MENESKEKVFANIKLDGKAITVKSLAYLLTFNLCSQLNLDMNLTESFY", "aa_hexdigest": "0c204871d5679cf8e28c8693b158fae8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5489.296399999999, "isoelectric_point": 5.05215663909912 }, "id": "EFPKPENHBP_1998", "locus": "GCA000186005_01995" }, { "type": "cds", "sequence": "contig_1", "start": 1093008, "stop": 1093490, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTCAGATATCCATAACTTCAGAAGCACTAACACAATGGGTGATGATATTGCAGAAGAATTCAAAAAAGATTACAACAATAAATTTGAAGAAATTGTTAAATATGTAAACAGTTTATTGCAAAATAATGTATTTAGCAAAATCAAAAATCAAAACAATTTAGCTTTAAATGCATTCAAAGCTTTAAAACAAACATTAGGTGAAAACAACAAGATAGCTCAAGAATTAGCTTATGCAGGTTGTTCAATTCACTATCAAGAAATTGGTTTTGATCCTTACATTGAAAGTCAATTAGATTTATTAAGAAATAATTCAGATCTTGAAATATTCTTTAAAACAAAAAAACCTAATGAAAGTAGATATTTAATTACTTATGTTCGTAAAGCATTGAAAAACAATAGAAGTAAACTATTTGCATTCTTAATTTACAAAAACTTATTCTCACATGAATACAGCAACCTATTAGAAGAAAAAGGTTGA", "aa": "MYSDIHNFRSTNTMGDDIAEEFKKDYNNKFEEIVKYVNSLLQNNVFSKIKNQNNLALNAFKALKQTLGENNKIAQELAYAGCSIHYQEIGFDPYIESQLDLLRNNSDLEIFFKTKKPNESRYLITYVRKALKNNRSKLFAFLIYKNLFSHEYSNLLEEKG", "aa_hexdigest": "d930a71b45af3f18d3eb8310c2a904c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18801.042700000005, "isoelectric_point": 8.48785915374756 }, "id": "EFPKPENHBP_1999", "locus": "GCA000186005_01996" }, { "type": "cds", "sequence": "contig_1", "start": 1093545, "stop": 1094057, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGTTGGATTCTTAGCAATTGCTATTTATTATGGTAGAAACTATTTATTAGACTTAAAAAAATCAAAAAATATAGTAGTTCAAGAAACTAAAGAAATAGTAGTTATTGAAGATAAAGAAAAACTTGTTAACTTAATTGACAAATCTGGCTTAGATGCTTTGATTATAGAAGATTTAGGCGAATCTAAAGATTATCAATTAATCAAAAGAATTAAAGATATTAACACACTTGTAAACAAAAGAAGACAAAAAAGAATTGAAAAAATTTTAGCTAAACTTGAAAATAAAAAAGAACAAATTGCTAAATTTTATGCATCAAAATACTATGTTAAAAAATGTAAAGCAATTAAATTCTTCTATGTAATTGACAACACACTTGCATCACTAGAAGACAAAGGTAATATTTTTGATTTTTCATTAACTGTTTTACCAATTAGCAAAGAATACAAAAAAGAACAAATCAATAAATTGTTATCACAATCAAATTTAAGTGTTAAAGAATTAAGCTGA", "aa": "MIVGFLAIAIYYGRNYLLDLKKSKNIVVQETKEIVVIEDKEKLVNLIDKSGLDALIIEDLGESKDYQLIKRIKDINTLVNKRRQKRIEKILAKLENKKEQIAKFYASKYYVKKCKAIKFFYVIDNTLASLEDKGNIFDFSLTVLPISKEYKKEQINKLLSQSNLSVKELS", "aa_hexdigest": "f535557940026773864f16059ccecbb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19756.0293, "isoelectric_point": 9.457657051086429 }, "id": "EFPKPENHBP_2000", "locus": "GCA000186005_01997" }, { "type": "cds", "sequence": "contig_1", "start": 1094358, "stop": 1094624, "strand": "-", "frame": 2, "gene": null, "product": "YobI-like P-loop NTPase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527198.1", "SO:0001217", "UniParc:UPI0001F5998B", "UniRef:UniRef100_A0AB32XDD0", "UniRef:UniRef50_C4XFP0", "UniRef:UniRef90_C4XFP0" ], "nt": "ATGTCACAAAATAATAATTCTTCTTCTTTTACAAATCACCAATGTATTAGAAATGGAAACATCCAAAATAAAGTTCAAAATGAAGAACATATTATTGAAACTATAGAGCTTTATTCAACTGAAGACATGAAAAATTCTAATGCTTTCAAGTTATATAAAAAATCACTTGATTTTGCAATGAGTAACAACAATGACAAAGTGAATAATATTGCTATTATAGGTTCTCAAGATGGACAAAATCAAAATGTTATTGAAACATATAAATAA", "aa": "MSQNNNSSSFTNHQCIRNGNIQNKVQNEEHIIETIELYSTEDMKNSNAFKLYKKSLDFAMSNNNDKVNNIAIIGSQDGQNQNVIETYK", "aa_hexdigest": "8f35698b426c44eb60d180eea03e8e97", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F5998B", "ncbi_nrp_id": "WP_013527198.1", "uniref100_id": "UniRef100_A0AB32XDD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5998B", "RefSeq:WP_013527198.1", "UniRef:UniRef100_A0AB32XDD0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDD0", "uniref90_id": "UniRef90_C4XFP0" }, "psc": { "uniref90_id": "UniRef90_C4XFP0", "product": "YobI-like P-loop NTPase domain-containing protein", "uniref50_id": "UniRef50_C4XFP0" }, "pscc": { "uniref50_id": "UniRef50_C4XFP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP0" ], "product": "YobI-like P-loop NTPase domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_2001", "locus": "GCA000186005_01998" }, { "type": "cds", "sequence": "contig_1", "start": 1094973, "stop": 1095374, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33", "PFAM:PF12706.13" ], "nt": "ATGAATCCTACAAGATTAATACCTATTGGTGGAGTTCAAGAAATTGGTAAAGCTGCACTAATAGTTGAATACAAAAACCAAATATTTATTATTGATGCAGGTATCAAATTTGCTGATACAGCTTTAACTGGTATTAAAGGTATTATTCCTGACTATACATATTTGGCAGAAAGATATAAAAATATTGAAGGTCTTTTTATTACTCATGGTCACGAAGACCACATTGGTGGTGTTATTCATTTAATGAAACAAGTAGCAGTTAAAAAGATTTTTGCACCTAGAATTGCTATTCAATATTTGAAATTAAAATTCGATGAAAATAAAATTAAAAACAAGGTTGAATTTATTGAAATTGAAAAAAGTGCTGTACACACTTTTGGCGAATGTTCAGTAGATTTTTGA", "aa": "MNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDF", "aa_hexdigest": "e42671c62ea5b72bd2352300067aefc8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 78, "aa_cov": 0.5864661654135338, "hmm_cov": 0.9102564102564102, "evalue": 4.74284339075561e-10, "score": 36.02237319946289, "start": 19, "stop": 96 }, { "id": "PF12706.13", "name": "Lactamase_B_2", "length": 80, "aa_cov": 0.5714285714285714, "hmm_cov": 0.9875, "evalue": 1.0678261764174447e-08, "score": 31.275922775268555, "start": 55, "stop": 130 } ], "seq_stats": { "molecular_weight": 14972.367099999985, "isoelectric_point": 7.844658088684083 }, "id": "EFPKPENHBP_2002", "locus": "GCA000186005_01999" }, { "type": "cds", "sequence": "contig_1", "start": 1095438, "stop": 1096535, "strand": "+", "frame": 3, "gene": null, "product": "Ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27", "UniRef:UniRef90_C4XFN8" ], "nt": "ATGTGTACCGGTGACTTTAGATTTGATTACAACCCTATTGGTGAAATTTATACTGATTTTGCTCGTTTAGATCAAATTGGAAAAGAAGGCCTAACTGTTCTTTTGTCAGACTCAACTAATGCTATGCGTCCTTTCCACTCGCCTAGTGAAAATGACATCTTAACAGATATTGAAAAATACATGCGTCAAGCTACAAGAAAAACTATTATTACAGCTTTTGCTTCAAACTTAACTAGAGTTACAGCAATTATTGAGTTAGCTGTTAAACTTGGCAAAAAAGTTGCAGCTTTTGGTCGTTCAATGGTTCAAGGAATCAAAATTGGACGTAAATTAGGTTACATTAAAGCGCCTGCAAGTGTTTTTGTTGATAAAAAAGATGTATCAAAAGTTCCTGACAAAGATCTTGTTATTCTTACAACAGGTTCACAAGGTGAACAATTGGCTGCTCTTTCAAGAATGTCTTATGGTAAACACCAAAGCGTAAAAATTGAAAAAGGTGACATGATTATCTTTTCATCAAGTCCAATTCCAGGGAATAGAATGGTTATTGAACTTTTAGTTAATAGACTTTCAAAACTTGGAGCTATTATTAAAGAAAATGGTGTTGATGGTTACTTGCATACCTCAGGTCATGCTTACAAACACGAACATGATAAAATTTTCCAACTTACTAAACCAAAATACTTTTTACCTTACCATGGTGAATTCAGAATGAGTATAGTTCATGGCCAAAGCGCTATTAGAAATGGAGTTAAACCAGAAAATATAATGATTCCTGAAATTGGTCGTGTTTACAACATGATTAATGAAGAAATTACACAAACAGATGAAAAAATAGATTGTGGTCCTATTTATATTGATGGACACACAACCTTGAATTTAAGTGCAAATATACTTAAGGAAAGAGCTAAATTAGCTGATAGCGGTTTTGTAAATATGATCCTAAATATTAACAAAAAAACTAATTCAATAGTTGGCCGTCCAAGTTTAATTAGTCGTGGGAGCTTCTATGTAAAAACTTCGCTTGAATTAGTTGAAGAAGCTAAAAGAATTGCTCATGGTGCTGCTCTTTTCTATATTAAAAATACACCTGATTGA", "aa": "MCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPD", "aa_hexdigest": "a545fda8d8fe4ce8f64bce770d4f7502", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1095138, "stop": 1096814, "observations": { "mutation": [ 1095372, 1096533 ], "stop": [ 1095372, 1096533 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFN8", "query_cov": 1.0, "subject_cov": 0.5667701863354038, "identity": 1.0, "score": 716.0, "evalue": 3.3e-256, "gene_start": 1, "gene_end": 365, "reference_start": 187, "reference_end": 551, "reference_sequence": "MYFYIFIIILHNRSIKVLLAVFLNIKKGDQNMNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDFWTAQHSIPDAFGIRIKTPNGSIMCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPDWNIVDLKQLIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE" }, "paralog": false, "identity": 0.996415770609319, "score": 1092.0, "evalue": 0.0, "description": "Internal stop codon at 1095372, 1096533. Nonsense mutation around 1095372, 1096533.", "psc": { "uniref90_id": "UniRef90_C4XFN8", "product": "Ribonuclease J", "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFN8", "UniRef:UniRef50_Q6KH27" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27" ], "product": "Ribonuclease J" } }, "id": "EFPKPENHBP_2003", "locus": "GCA000186005_02000" }, { "type": "cds", "sequence": "contig_1", "start": 1096557, "stop": 1096814, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTATTGATCGTCTTGAAAATCTTTTCTACAAAGAAAAAAGAAGAAGACCAATTGTTGTGGCAACTATTCTTTTTACAGATGAAGAAAATGAACCAGCATTTGCAACTTCTAAAATTAAATTTAATGTTAAGGGTACAAAAGAAGATGAGAAAAGCAAAAAAGCTACTGCTGCTCTTAAAGAAGCAAAATCAGAAATGTTTGGTGAAAACAGTGAAATGTCAGAAGACATTCATGATGAAGAAGACGAAGAATAA", "aa": "MIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE", "aa_hexdigest": "3f4bd52e31ced8ad13e37a6918a462b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9870.935000000003, "isoelectric_point": 4.824345970153809 }, "id": "EFPKPENHBP_2004", "locus": "GCA000186005_02001" }, { "type": "tRNA", "sequence": "contig_1", "start": 1096895, "stop": 1096970, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 75.9, "nt": "GCACCGTTAGCTCAGTCGGTAGAGCAGCTGGCTCTTAACCAGCGGGTCACAGGTTCGAACCCTGCACGGTGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 1096935, 1096937 ], "id": "EFPKPENHBP_16", "locus": "GCA000186005_02002" }, { "type": "cds", "sequence": "contig_1", "start": 1097272, "stop": 1097658, "strand": "-", "frame": 1, "gene": null, "product": "DUF4064 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355056.1", "SO:0001217", "UniParc:UPI0001A8C3E2", "UniRef:UniRef100_C4XFN7", "UniRef:UniRef50_C4XFN7", "UniRef:UniRef90_C4XFN7" ], "nt": "ATGAATAAAAAAAGTTTTAAATCAACTTTAACTTCAATTGCTATAGCAGCAATTTTATTATTAACACTAACTATCTCATTTTTTGGATTACAAAAAAATATCCCAGAAGCAGTAATAAAAGCGACAAATAACAATTATGTGGCTGGAATTACTAACATTATTTTATTAGTTATTACTCTTGCTCTATGCATTTCTTTTATTGTTGTTGGTTCAATTCAAGCAGCAAAATTAAGTTTTCTTAAAAAAGATCGTTACATTTATGTTTTAGCAATTTTAGGAATTTTCTTTGTCCCTGTTTTCTATGTAGTAGCAGGAATTATTCTTGCTAAAAGAGATTCTATTGTTAATGAATTAGTTGATGAAGAAAATGAAGCAATTGAAGTTTAA", "aa": "MNKKSFKSTLTSIAIAAILLLTLTISFFGLQKNIPEAVIKATNNNYVAGITNIILLVITLALCISFIVVGSIQAAKLSFLKKDRYIYVLAILGIFFVPVFYVVAGIILAKRDSIVNELVDEENEAIEV", "aa_hexdigest": "fb0c22528d5377771443af7c318b9197", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C3E2", "ncbi_nrp_id": "WP_013355056.1", "uniref100_id": "UniRef100_C4XFN7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3E2", "RefSeq:WP_013355056.1", "UniRef:UniRef100_C4XFN7" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN7", "uniref90_id": "UniRef90_C4XFN7" }, "psc": { "uniref90_id": "UniRef90_C4XFN7", "product": "DUF4064 domain-containing protein", "uniref50_id": "UniRef50_C4XFN7" }, "pscc": { "uniref50_id": "UniRef50_C4XFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN7" ], "product": "DUF4064 domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_2005", "locus": "GCA000186005_02003" }, { "type": "cds", "sequence": "contig_1", "start": 1097858, "stop": 1098460, "strand": "+", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "RefSeq:WP_013527199.1", "SO:0001217", "UniParc:UPI0001F5998C", "UniRef:UniRef100_C4XFN6", "UniRef:UniRef50_A0A3P8KMF6", "UniRef:UniRef90_A0A3P8KMF6" ], "nt": "ATGGCAAAATCAAAAGCTGATATTGTCCGTGTTCGTAAGCTACAATTCATCGCTGGCCAAGCCAAACCTGGTCCAGCTTTAGCTGGTGTTGGAATCAATATGCCTGATTTCACACGTGCTTTTAATGACGCAACACGTGATAGAGGTAATGAACCAGTTCCTGTTGAAATTACCGTTTACAAAGATAAATCATTTGACTACAAGTTATTTACAGCTCCAGCTTCGTACAAATTAAAGCAAGCTGCTAAATTAAACAAAGGTAGTTCAAACTCAAAAACAACAATTGCTGCTACTATTACAAAAGAACAATTGAGAGAAATTGCTGAATACAAATTACCAGACTTAAACAAAAATGATGTTGAAGCTGCTATGAGAACTATTGCTGGAACAGCAAGACAAATGGGTATTATTATCGAAGGATTTGATAACGTTGCTGCTAAAAAAGCTGCTGCTGCTAAAGCTGCAAAAGACAACGCAGCTGCTGCAGCTAAAGAAGCCGCTTTAGATGAAGCTCAAAACGAACTAAAAGAATCTAAAGGTAAAACCATTGATGTTAAAACAATTTCAGACGAAAAAGCTGCTGCTAAAGAAGGAGAAGAATAA", "aa": "MAKSKADIVRVRKLQFIAGQAKPGPALAGVGINMPDFTRAFNDATRDRGNEPVPVEITVYKDKSFDYKLFTAPASYKLKQAAKLNKGSSNSKTTIAATITKEQLREIAEYKLPDLNKNDVEAAMRTIAGTARQMGIIIEGFDNVAAKKAAAAKAAKDNAAAAAKEAALDEAQNELKESKGKTIDVKTISDEKAAAKEGEE", "aa_hexdigest": "933d8bed8a9340a5efe79a2164d8641c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F5998C", "ncbi_nrp_id": "WP_013527199.1", "uniref100_id": "UniRef100_C4XFN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5998C", "RefSeq:WP_013527199.1", "UniRef:UniRef100_C4XFN6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN6", "uniref90_id": "UniRef90_A0A3P8KMF6" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KMF6", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_A0A3P8KMF6", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KMF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KMF6" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "EFPKPENHBP_2006", "locus": "GCA000186005_02004" }, { "type": "cds", "sequence": "contig_1", "start": 1098460, "stop": 1099155, "strand": "+", "frame": 1, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_013355058.1", "SO:0001217", "UniParc:UPI0001E33088", "UniRef:UniRef100_C4XFN5", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_C4XFN5" ], "nt": "ATGTCTCTTAAAGGTGGAAAGAAAATAAGAAATGCTCGTGAATCATTTGACAAAACAGTTGCATATGATTTAAAAGAAGCATTAGAAATTGTTAAAAGAACATCATTTACAAAATTTGTTCCTACAGTAGAATTAGTATTCAAATTAAACCTTGACGTTAGAAAAGCTGACCAACAATTGCGTGGATCAGTATTGTTGCCTAACGGTACAGGTAAAAGCGTTCGTGTATTAGTTGTTTCAAACAATCCAGCAATTCAAAAATCATCAAAAGCTGCTGGTGCAGACATCGTTGTTGATGGTCCAGCACTTGAACAAAAAATTAAAGAAGACGAATTTGACTTTGATGTTATGGTTGCTGACCCTTCAATGATGCCTTTATTAGGTAAATATGGTAAAAAACTTGGACCTAAAGGTTTAATGCCAAACCCTAAAACAGGTACAGTTACACCTACTCCTGATAAAACAGTTGCTGAACTTAAAAAAGGTAAAGCTAACTACCGTACAGATAAAGCAGGTATTGTACATACAATCATTGGTAAATTAAATATGACAACAGACCAATTAGCAGAAAATGCTCAAACAATCATTAATTTAATTAAAAAACTTAAACCAGCTACAGTTAAAGGTGCTTACATGCAAAACTTAGTTGTAACATCAACAATGGGTCCTGGTGTTAAAGTTAAAATTGAAAAATAA", "aa": "MSLKGGKKIRNARESFDKTVAYDLKEALEIVKRTSFTKFVPTVELVFKLNLDVRKADQQLRGSVLLPNGTGKSVRVLVVSNNPAIQKSSKAAGADIVVDGPALEQKIKEDEFDFDVMVADPSMMPLLGKYGKKLGPKGLMPNPKTGTVTPTPDKTVAELKKGKANYRTDKAGIVHTIIGKLNMTTDQLAENAQTIINLIKKLKPATVKGAYMQNLVVTSTMGPGVKVKIEK", "aa_hexdigest": "54a24d6dbe97b10a979f8eab9baf07d5", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33088", "ncbi_nrp_id": "WP_013355058.1", "uniref100_id": "UniRef100_C4XFN5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33088", "RefSeq:WP_013355058.1", "UniRef:UniRef100_C4XFN5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN5", "uniref90_id": "UniRef90_C4XFN5" }, "psc": { "uniref90_id": "UniRef90_C4XFN5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "EFPKPENHBP_2007", "locus": "GCA000186005_02005" }, { "type": "cds", "sequence": "contig_1", "start": 1099243, "stop": 1099752, "strand": "-", "frame": 1, "gene": null, "product": "Aminotransferase class V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3", "UniRef:UniRef90_A0AB32XD79" ], "nt": "ATGCCTGGTTTAAGTTGTGTTGTTTTATCTAAAAGAGCTCAACAAGTAGTTAAAGCAAATAAAAACTTTAAATTCTATTTTGATTTATCAGCTTATATTAATTTTTATGAAAGAGAAAAATCAACTCCTTATACTCCAGCTTCAGGTATTTTATTAGCTTTGAATGCTGCAATGAAAGTTATCAAAAACCAAACATTTAAGAATATAAGAAATAATAAACTCAAAATATATGACTACATTAAAAAAGAATTAATTGCTATTGGATTTAAGGATGTAGTTCCTAAAAAGAATATAACTATTGGACTTTTAGTTTTAAATGTTCCAGAAGGTGTTGATGCAATTAAGTTAAGAAATAAAATTGAATATAATCACAATATTTATTTTGAATTAGGTCGTGGAAATAGAAGAAGTAAACAAGTCAGAATTGGAATTCCTAATACTATAGACATGGAAAAAGCTAAAAAACTTGTTAAAGTAATTAAGGAAACTTTAAAGATTAAAATTGATTAA", "aa": "MPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID", "aa_hexdigest": "f1da88a11b553b96b404e2e7f0324978", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1099243, "stop": 1100052, "observations": { "mutation": [ 1099800 ], "stop": [ 1099800 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD79", "query_cov": 1.0, "subject_cov": 0.49415204678362573, "identity": 1.0, "score": 327.0, "evalue": 1.68e-110, "gene_start": 1, "gene_end": 169, "reference_start": 174, "reference_end": 342, "reference_sequence": "MLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDNWGLDVAIATSGKAFCVMPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID" }, "paralog": false, "identity": 0.9962825278810409, "score": 528.0, "evalue": 2.07e-192, "description": "Internal stop codon at 1099800. Nonsense mutation around 1099800.", "psc": { "uniref90_id": "UniRef90_A0AB32XD79", "product": "Aminotransferase class V", "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD79", "UniRef:UniRef50_A0A3P8MEM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3" ], "product": "Soluble hydrogenase 42 kDa subunit" } }, "id": "EFPKPENHBP_2008", "locus": "GCA000186005_02006" }, { "type": "cds", "sequence": "contig_1", "start": 1099798, "stop": 1100355, "strand": "-", "frame": 1, "gene": null, "product": "Aminotransferase class V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3", "UniRef:UniRef90_A0AB32XD79" ], "nt": "ATGCAAACCACGCTTCTACTTTATTATTTACACCAGGTCCTATTAAATAATTATCCAGAAGTTTCTGAGATTTTATCTGAGTCAGTGTTGCATCATAGAAGTGAGTTGTTCAAACACTACATGGAAGAATCAACTAATTTAATTAAAGAATTCTTTAGAACTAAAGAAGCACTTCCATTATTTATTACATGTTCAGCAACTGGGGCTATGGAAGCAGTTGTTGTTAACCTTATTAGTCCTGAAAACAAAGTTTTATTGGTTGAAGCAGGTGACTTTGGTAAAAGATTCCAAATAATTTTAGAAAGATTAATTGGAGCTAATAATCTTGATGTTATTCATTATGAAGATGGTGAAGCATTCAAAGTAAAAGATATAGAAAATAAACTTAAAGAAAATAAATATGATGCATTCTTCTGTACTCATCATGAAACATCAACAGGTGTTTTAAATGATATTGAAGCTATTTCAAAAGTAGTTAAAAAATATGCTAAAGATACATTATTTATTGTGGATACAGTTAGTTCATTTATTCATGAAAATGTTGAGTTTGATAATTGA", "aa": "MQTTLLLYYLHQVLLNNYPEVSEILSESVLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDN", "aa_hexdigest": "120fdba55ab72cf5121941653a3370f9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1099495, "stop": 1100271, "observations": { "mutation": [ 1099800 ], "stop": [ 1099800 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD79", "query_cov": 0.8486486486486486, "subject_cov": 0.4590643274853801, "identity": 0.9940000000000001, "score": 310.0, "evalue": 7.66e-104, "gene_start": 29, "gene_end": 185, "reference_start": 1, "reference_end": 157, "reference_sequence": "MLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDNWGLDVAIATSGKAFCVMPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID" }, "paralog": false, "identity": 0.9922480620155039, "score": 507.0, "evalue": 1.27e-183, "description": "Internal stop codon at 1099800. Nonsense mutation around 1099800.", "psc": { "uniref90_id": "UniRef90_A0AB32XD79", "product": "Aminotransferase class V", "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD79", "UniRef:UniRef50_A0A3P8MEM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3" ], "product": "Soluble hydrogenase 42 kDa subunit" } }, "id": "EFPKPENHBP_2009", "locus": "GCA000186005_02007" }, { "type": "cds", "sequence": "contig_1", "start": 1100306, "stop": 1100434, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGGAAGCAATTTCTTCAAATTTGATTAAAGAACTTGACAATTTTCAAGACTTAATTAATATTGATCATCATTATGCAAACCACGCTTCTACTTTATTATTTACACCAGGTCCTATTAAATAA", "aa": "MRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPIK", "aa_hexdigest": "583c00c14d854c5a84dcb60a1f317854", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4791.398799999999, "isoelectric_point": 5.747695350646972 }, "id": "EFPKPENHBP_2010", "locus": "GCA000186005_02008" }, { "type": "cds", "sequence": "contig_1", "start": 1100636, "stop": 1101058, "strand": "-", "frame": 3, "gene": null, "product": "Nucleotidyl transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2", "UniRef:UniRef90_A0AB32XDA2" ], "nt": "ATGGCAAAAGTTAGAAATGCTGTTATTTTTGCAGCAGGTAAGGGGACTAGAATGACCCCATTAACTCATTATGTTCCTAAACCATTAATAACTGTAAATGATGAATCAATGATTGAAAGAAACATAAAGCATTTACAAGAAATAGGCATTAATGACATCACTGTGGTTGTTGGTTATATGAAAGACCAATTTAATTTAATTAAAGAAAAATATAATGTCAAATTAATTGAAAATGATTTATATGATGAAGCAAACAACATTTATTCTTATGTTGTTTCCAAAAATGCATTTGGAGACACTTTATATATTGAAGGTGATTTGTATATTGAAAAAAATATTTTTCCAGAAATAATTAAAATAATTGAAAACTCTGAAGATTCAATTTGTTTTACTGAAAAATGTACAAAACACAAATCGGAATGA", "aa": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSE", "aa_hexdigest": "723778bd553b296ea32e140b7f45037c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 1100333, "stop": 1101058, "observations": { "mutation": [ 1100452, 1100500, 1100563, 1100638 ], "stop": [ 1100452, 1100500, 1100563, 1100638 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDA2", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 281.0, "evalue": 2.09e-94, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSEWVFDTNNKGYVVKHHNEKDAYNQNIWIGILYLNKKLANEAKEKVDAWFNQQGNKQQYFETFLWTLENKMRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPIK" }, "paralog": false, "identity": 0.983402489626556, "score": 473.0, "evalue": 2.79e-172, "description": "Internal stop codon at 1100452, 1100500, 1100563, 1100638. Nonsense mutation around 1100452, 1100500, 1100563, 1100638.", "psc": { "uniref90_id": "UniRef90_A0AB32XDA2", "product": "Nucleotidyl transferase", "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDA2", "UniRef:UniRef50_A0AB32XDA2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2" ], "product": "Nucleotidyl transferase" } }, "id": "EFPKPENHBP_2011", "locus": "GCA000186005_02009" }, { "type": "cds", "sequence": "contig_1", "start": 1101108, "stop": 1101278, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTCTTTTCGATATTAGGTGATTTATACTTTTCATATGTTAAAAGAATAAATGCGATAAAAGATTATTCAAAAATTTTAATAAGTCATGGTGGTATTTTAGATCGTTTTGATAGTGTTGCTTTAGGAGGATTAACTTTTCTTTTGTTGTTTGTTATATTTATATAA", "aa": "MPFFSILGDLYFSYVKRINAIKDYSKILISHGGILDRFDSVALGGLTFLLLFVIFI", "aa_hexdigest": "16c6ce4dbdaf0ed75b8b19a0bce215ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6359.517399999997, "isoelectric_point": 8.1192289352417 }, "id": "EFPKPENHBP_2012", "locus": "GCA000186005_02010" }, { "type": "cds", "sequence": "contig_1", "start": 1101519, "stop": 1101671, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTGTTGCTTTTATTATTATTGAATATATAACAAGAAATAATATGAGTTTTGAAGATCGTTTGGTTAGAGGAATACTAATGTTTATTTCTCTATACGTAATAGTCAACACTTCAAAATTGATTCAAATAACCATTATACTTGATTGA", "aa": "MISVAFIIIEYITRNNMSFEDRLVRGILMFISLYVIVNTSKLIQITIILD", "aa_hexdigest": "985fba476d0b234caad08870561bcc04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5834.993599999998, "isoelectric_point": 6.045929527282715 }, "id": "EFPKPENHBP_2013", "locus": "GCA000186005_02011" }, { "type": "cds", "sequence": "contig_1", "start": 1101693, "stop": 1101806, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATACTACCTACTGTAGTTTTACCGGTTAATAACTTTACTGAGTTTATTTTTGTTTCTCATCCTTTAGAATTTCATCCTAGTTTTATTCAAGAAATAGCAAAAGAATGA", "aa": "MLILPTVVLPVNNFTEFIFVSHPLEFHPSFIQEIAKE", "aa_hexdigest": "ad9ae4d054a84fca641b04350f50ab53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.0075, "isoelectric_point": 5.015893363952636 }, "id": "EFPKPENHBP_2014", "locus": "GCA000186005_02012" }, { "type": "cds", "sequence": "contig_1", "start": 1101828, "stop": 1101950, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACCTTTAGCAATTTTTGGAAAAAATCATTATGAAGCTAGAATAGTTTCATATATTTTTACTGCTATTGGGATAGGAATACTATGCTATGAATATTTCAAAGCTAATCATTTAAAATGA", "aa": "MVPLAIFGKNHYEARIVSYIFTAIGIGILCYEYFKANHLK", "aa_hexdigest": "887ccf7e2e2e6a56cadadfc2068deb6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4605.4251, "isoelectric_point": 8.932368659973147 }, "id": "EFPKPENHBP_2015", "locus": "GCA000186005_02013" }, { "type": "cds", "sequence": "contig_1", "start": 1102135, "stop": 1103220, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355061.1", "SO:0001217", "UniParc:UPI0001E3308B", "UniRef:UniRef100_A0AB32XDL5", "UniRef:UniRef50_C4XFN2", "UniRef:UniRef90_C4XFN2" ], "nt": "GTGTCTGAAGAATTAGAAAACAAAGAAAAAAAAGTTAAAAAAGATCAAACTTCTACTAAAAAATCAACAGCCAAAAAAATGACCAAAAAGTCTGCAAAAAAGGCTGAAAGAGAAAAAAATAAAAAAGAAGAAACTGGTCTTTTACAAGCTATTATTGAAGAAGAAAAACTTCTTGAAGAACAAGTCGCTGAATTAGAAATTGACAAAAATATCAAAATTACTAAAGGCAATGTTTTTGAGCTTTTAGACAGAGATAGTTCTAAAAAGAATATTATTGTTCCTAAAAAAGTTCGAAAATTATTGACTAAGTTAGCTAAGAAAAAAAGAAAAAAAGATGGCAATGAAAACCTAAAAAATGAAGAAGGTAATGTTATTGAAGTTAAGAACGTTAGCAAATATTATGTTAATGGTAATATTGTTACAAGAGTTTTAAAAGATGTTTCAATTGAAATTAAAAAAGGTGAGCTAATGCTTATTTATGGTGTTAGTGGTGGTGGTAAAAGTACACTTCTAAACCTTATTTCAGGACTTGATAGACCTTCAAAAGGACAAGTTATTGCTTGCGATAATAATCTTCCTTATATGTCTGATAGTCAATTAACTCTATTTAGAAGAAAACATGTTAGTTTCATTTTCCAAAACTATAACTTGTTAGCAAACTTAAATGCTTTTGACAATGTTCAAACGGGAGCTTACTTACAAAAAAATCCTGCTAAAAAAGTTGATGTTGTTGAGTTATTTAAGAAATTTGAAATGGATGAAGAAATATCAAAATTCCCTGCTCAAATGTCTGGTGGTCAACAACAACGTGTTTCAATTATGAGAGCCTTAGCTAAAAATGCAGATATCATTTTTGCTGATGAACCTACTGGTGCTCTTGATGAAGCAACAACAAAAATAGTTTTAAGATTTCTTTACAATATTAATAAAGAAACAGGCGCTACAGTTGTTATGGTTAGTCATAACCCAGTTATGGCGCAAATGGCTGATAGAATTATTCATGTTGTTAAAGGAAGAATTGCAAAAGTTGAAGTTAATCAAAAACCAATTCACCCTGATGATATTGAACTTTTTAAAGAAAAATAA", "aa": "MSEELENKEKKVKKDQTSTKKSTAKKMTKKSAKKAEREKNKKEETGLLQAIIEEEKLLEEQVAELEIDKNIKITKGNVFELLDRDSSKKNIIVPKKVRKLLTKLAKKKRKKDGNENLKNEEGNVIEVKNVSKYYVNGNIVTRVLKDVSIEIKKGELMLIYGVSGGGKSTLLNLISGLDRPSKGQVIACDNNLPYMSDSQLTLFRRKHVSFIFQNYNLLANLNAFDNVQTGAYLQKNPAKKVDVVELFKKFEMDEEISKFPAQMSGGQQQRVSIMRALAKNADIIFADEPTGALDEATTKIVLRFLYNINKETGATVVMVSHNPVMAQMADRIIHVVKGRIAKVEVNQKPIHPDDIELFKEK", "aa_hexdigest": "c33a614a06c283fe2031325307294454", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E3308B", "ncbi_nrp_id": "WP_013355061.1", "uniref100_id": "UniRef100_A0AB32XDL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3308B", "RefSeq:WP_013355061.1", "UniRef:UniRef100_A0AB32XDL5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDL5", "uniref90_id": "UniRef90_C4XFN2" }, "psc": { "uniref90_id": "UniRef90_C4XFN2", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFN2" }, "pscc": { "uniref50_id": "UniRef50_C4XFN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN2" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "EFPKPENHBP_2016", "locus": "GCA000186005_02014" }, { "type": "cds", "sequence": "contig_1", "start": 1103510, "stop": 1103881, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAACAAATTTATAATCCAGATACTGGAATATTAAAAACCAGATTGAATCTAACAAAAGATCAAATTATGAATTTATTAGATTCAACTGAATTAACATTTAATAAAGCTAAATATGATCAAATTAAAAATGCGCCTAAAAACGCAATAGAAAATTATGCTAAATTGTATGATTCTAAACATTATATTGCTTTAGGTACAAGTATTGATTCAAAAGATATAGAAGCTGGATTTACAACCCAAATTGGTTCTACTATAGGAACAATTAGTATTGCAATTATTGCAATTAGTTTCATAATTTCACTTGTTATTCTTATTATTATGTCAACAATTATGATTAGTGAAAATGAGAAAAACATTGCTATTTGA", "aa": "MFEQIYNPDTGILKTRLNLTKDQIMNLLDSTELTFNKAKYDQIKNAPKNAIENYAKLYDSKHYIALGTSIDSKDIEAGFTTQIGSTIGTISIAIIAISFIISLVILIIMSTIMISENEKNIAI", "aa_hexdigest": "7f65b0cc7a1b904e96c718b69efd4c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13690.737799999984, "isoelectric_point": 5.278318977355956 }, "id": "EFPKPENHBP_2017", "locus": "GCA000186005_02015" }, { "type": "cds", "sequence": "contig_1", "start": 1103936, "stop": 1104295, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGTAAATGAAGTTACTTCGAAGAAATATAACATTAATATAGGCGATTTAGTCCAATTTGAAGTTCTTAACCATACAAAACGTTATGCAAACAAAGTGTTGTCAAAAATAGATAAATCATTAAGAAGTAATGTTGACTTAAAAAATCCAAAAGTAACATTTAAAGTTGTTGGTATTAACCCTACATACATTAATAATGAATTAATAACAACAGTTGATGCAGCTAATAAATTAGTTGGCTTAGACAAACTTCCAAATGTTGTTGGATTTAAAGCCTTCAATGGTGTTATGACAAATAGTCCAGCGCCTAGCCAAGTTCTTGATTCAACTTCGCTTTATTCATTAAGTGGTTATTGA", "aa": "MVVNEVTSKKYNINIGDLVQFEVLNHTKRYANKVLSKIDKSLRSNVDLKNPKVTFKVVGINPTYINNELITTVDAANKLVGLDKLPNVVGFKAFNGVMTNSPAPSQVLDSTSLYSLSGY", "aa_hexdigest": "f0482b84fd1f95324d9ceab74f8b55fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13089.918299999987, "isoelectric_point": 9.487763786315917 }, "id": "EFPKPENHBP_2018", "locus": "GCA000186005_02016" }, { "type": "cds", "sequence": "contig_1", "start": 1104539, "stop": 1104694, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATATAACAACCGATAAATATCGTGATGAATTTAGAACACTCTTAATCAATGGTTATACTAAAATTGCTCAAAAAATTAAAGCCGAAAAAGAAAATCCAAGTTTAATTAAACCTAAAGAAAAGAAACCAACCAATTTATCTGATTACTGA", "aa": "MVNITTDKYRDEFRTLLINGYTKIAQKIKAEKENPSLIKPKEKKPTNLSDY", "aa_hexdigest": "fa64605c08a83b0df022737fa660076d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5985.860800000001, "isoelectric_point": 9.522189903259278 }, "id": "EFPKPENHBP_2019", "locus": "GCA000186005_02017" }, { "type": "cds", "sequence": "contig_1", "start": 1104719, "stop": 1104934, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGATACTCAAAAAGCTAAAATTGATAAGATAAGAGACCGTGTTGGTTATGAGTTAGAAAGAACTCAAGAAGACAAAGATAAAGAATTCTTTGTAGATCCTAATACTTATGCTTTAAGCTTAGTAAATAAGGCTAATAAGGAAAAATTAAGCTTCTTTAAATACTATCATTCACCATATGAAAAACAAGGTAGTTTCAAATATGCTTACTGA", "aa": "MPDTQKAKIDKIRDRVGYELERTQEDKDKEFFVDPNTYALSLVNKANKEKLSFFKYYHSPYEKQGSFKYAY", "aa_hexdigest": "31a20d61db17d34b6d3ea38f3af27a1e", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8552.5075, "isoelectric_point": 8.777128791809083 }, "id": "EFPKPENHBP_2020", "locus": "GCA000186005_02018" }, { "type": "cds", "sequence": "contig_1", "start": 1104953, "stop": 1105309, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAGGTATCGCAACCTTATTTGGTGTAGCCAATACAAACGTCTTTAATAGAACTATTAATGATACTTATAAAAATAGAAGTTATACATTTAAAGTTGATTTAGAAAGTCCAACAGTTGAAGGTGGAGCATTCAAACCATTTAATCCTGATAATTTATATAACAACATTTATACTCCTCTTGGTGATATAAATGAAGGTAACAGAGAAATAGGAGATTACTTTAAACCTGGTGCTTCTAGTGTGCTTAATAAAGGAACTAAAAATGGTAGTCCTCAAGATTTAGACTCACATATCTTAAGCCAATTCTCAGTTAATGTTACTGTTGCAGCTGGTGTAGCCGCTGACCCTTGA", "aa": "MLTGIATLFGVANTNVFNRTINDTYKNRSYTFKVDLESPTVEGGAFKPFNPDNLYNNIYTPLGDINEGNREIGDYFKPGASSVLNKGTKNGSPQDLDSHILSQFSVNVTVAAGVAADP", "aa_hexdigest": "f5b39e5df9c5293b34a1be4edd37b25b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12720.910799999987, "isoelectric_point": 4.898975563049316 }, "id": "EFPKPENHBP_2021", "locus": "GCA000186005_02019" }, { "type": "cds", "sequence": "contig_1", "start": 1105337, "stop": 1105684, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTTTAGTTACATCAGTTATTGGTGGAATCTTAGGTTATGTAATTGGTTATAAACTACAACTGATGGTTATGAACATCTTTAGTAGTTATTGGACACTGCCAAAACAAACGATTTCATTTAACTTCTTTACATTATTCTTCACTGTCTTCTTACCATTTATTGGAATGAGCTTATTGATCTTTATAGTTTCATTATTTGCTTTAAGACATAAAGCTATAGACTTAATTAGTGGAGTTAATGAAATACCTCAATCAAAATTATTCAAAACTTATCAAAAAGCAACTAAGAAATTTAATGTTAAAAAACGTTTCTCATTTACATTGGCATTTACAAGTTTTTGA", "aa": "MFALVTSVIGGILGYVIGYKLQLMVMNIFSSYWTLPKQTISFNFFTLFFTVFLPFIGMSLLIFIVSLFALRHKAIDLISGVNEIPQSKLFKTYQKATKKFNVKKRFSFTLAFTSF", "aa_hexdigest": "0ee163dd4830ab23af7e54c735a63c13", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.757899999975, "isoelectric_point": 10.250618171691894 }, "id": "EFPKPENHBP_2022", "locus": "GCA000186005_02020" }, { "type": "cds", "sequence": "contig_1", "start": 1105706, "stop": 1106848, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAATTATTTATTAAGCAAAGATTTTTATGACATTAAAGACCAAGCTCAAATCATTATGTCTTCATTAAATCTTAATGTACCATTACAAATTTTTGGTTTAAGTGAAGCCTTAGTCAATCCTATTTTAAGATTTACTTTCCCTGAAATTACATTATCTTATTTAGCAAGTCAAAAAACAAATGCTAGTTTAGTTAAAGCTAACTTACAATACTTATTGCTCAATAAAATAGATGATTTCGAAAAAATTGTTAGAAAAGGCGAAGACAAATTTTATGCCTTAACTAACATGTTAGGAACTGCATTTAGCAAAAAAGATACCTCACTAATTCCTCTTGACTTAGATGACGAACAAACATTAGTAATGGATGGAGCTACATTTGATAAATTAAAAGATACAAGCTCTTCATTATTTGGAATTGATTTTATTAAATTTGTTAATGAAGCAATGAACACGATAGTTGAACCTAAAGAAATGAAGGACATTGTGTTCTCAAATTCAAATAGTTACTTAGCTAAAGTTAACTATGCATACTTAGCAAAAAATAATAAAGCAATTTATACAGGTAAAATTCCAAGTGATCCTATTGAACTATTATCATTATTAGATAAATTAGATGAAAAATTCATTTTAAATGTTAATGGAACCAAATTTATTATTATTGGTCAAGAAACTACAGTCGACTATATGTACCCAGTTATTGATGAAAATAACTTACAAGTTAATACACAAAACCAATCCTTAGTTTATGTTAACAAATATGGTTTTGACAGAATTCACTCAGGTTACATTGGAAATGTTATTAAGAAAACTCTTTTAGTTAAAAACTCAGCAGCAACTGGTATGAATAATAATGAACTTAAAAAATTCATTACTAAAATAATAGATAACTCAATTGCAGACAGTAACAAACTTCAAAGAGTTTTCTTAGCAAATGAAATTGATCCAATTAACCCTGAAAGGTCTCTTAGAATAACAACTGTTCAAGGAATTATTAAAGCTGTTTCATCAGCTACTATTGCCTTAGTTTCAGTATTCGTTACACTTGTGGCTATTTCAACTATCTTTATTATTAAGAGATATATTTCAAATAAAAACAAAGTTATTGGTATTTTAGTGGCCCAAGGTTATCGTCCATGA", "aa": "MNNYLLSKDFYDIKDQAQIIMSSLNLNVPLQIFGLSEALVNPILRFTFPEITLSYLASQKTNASLVKANLQYLLLNKIDDFEKIVRKGEDKFYALTNMLGTAFSKKDTSLIPLDLDDEQTLVMDGATFDKLKDTSSSLFGIDFIKFVNEAMNTIVEPKEMKDIVFSNSNSYLAKVNYAYLAKNNKAIYTGKIPSDPIELLSLLDKLDEKFILNVNGTKFIIIGQETTVDYMYPVIDENNLQVNTQNQSLVYVNKYGFDRIHSGYIGNVIKKTLLVKNSAATGMNNNELKKFITKIIDNSIADSNKLQRVFLANEIDPINPERSLRITTVQGIIKAVSSATIALVSVFVTLVAISTIFIIKRYISNKNKVIGILVAQGYRP", "aa_hexdigest": "1c27aa503d074da4304bab2b68231356", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42683.90679999998, "isoelectric_point": 8.625112342834473 }, "id": "EFPKPENHBP_2023", "locus": "GCA000186005_02021" }, { "type": "cds", "sequence": "contig_1", "start": 1106966, "stop": 1107235, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATGAATAAATATGCAAATCATGAAGAAGCAAAAAGATTTGTTAAAGATTTAATTGCATTAGCATTGGAACCTGAAATATTTGACATTAACAAAAAAATGTCAACAAATGACAATATACCTTTTGCTGGTGTAACTAACTATGGATTATTAGAATTACTTGAAAATCCCGAAAAATCAAACTTATTTGCTTATGATGTTAATGGCAAATTCTTAAATGACAAAGTTGAACAATTTGCTGCTAAAAATGAAATATATAGAAAATGA", "aa": "MFMNKYANHEEAKRFVKDLIALALEPEIFDINKKMSTNDNIPFAGVTNYGLLELLENPEKSNLFAYDVNGKFLNDKVEQFAAKNEIYRK", "aa_hexdigest": "df9ebb9feec64cabbf409eb29d0c0f55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10334.68269999999, "isoelectric_point": 5.366248893737793 }, "id": "EFPKPENHBP_2024", "locus": "GCA000186005_02022" }, { "type": "cds", "sequence": "contig_1", "start": 1107338, "stop": 1108120, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTCATTAATTAGTGGTTTAAAACCTATTATTAAAAAAATGAATAGCGATCCAACAAATCCTTATTCTAATGTAAAACAAGGCTTAATTAATATTCTATCAAATATTGATTTTAATATTCTGGCTCGTGAATTAAATCAAACAATAACAACACATTATATTGATTACAAAGTAGTTAAATTTAATCCAGACTTAAACAAAGATGAAGAAAAAGTCTTTTCAGTTGTTTTAAATGCAATAAGTCCAAAAGATGGAATTATATCATTCTTAAGATCTATGTTTGGTATGCCTGGATCAAATAGAGCATTTAAAGAAAACTTAATCAAAATGTTCAATTTGTCTGACAAATATAAAGAAATAGAACTACCTAATAGTAGTGTAAAAATTTATGTTCCAGATAATGACGACACAAAAGTAGGTTTCTTTGATTTCTTAAAAATTTTTGCTTCAGGTTTATCAAATACAGAAGCTAATGAATTCAATAACTACAAAATTGAACATGACTTCTTAGCACTTAAATCATCAATTGAAAGTCTTAAAGAGGAAGGCACTCAAATTAATTTAGATTACTTAAAAGAACCTCAAAAAAGATTACTTAAACAATATGGTTTATTTGTCACAAAAACCTATAAAACAGTCATATTAGAAAAAATTAATAAAATATTAAGGTTCATAAAACAAACAAAAGGTGGAACTTCATATATAAGTAATGATGCTAATAAAACAGGTAGTGATTTATTAAATGATTTAAAAAATTTTGAAAACAACGGGACTTGA", "aa": "MNPSLISGLKPIIKKMNSDPTNPYSNVKQGLINILSNIDFNILARELNQTITTHYIDYKVVKFNPDLNKDEEKVFSVVLNAISPKDGIISFLRSMFGMPGSNRAFKENLIKMFNLSDKYKEIELPNSSVKIYVPDNDDTKVGFFDFLKIFASGLSNTEANEFNNYKIEHDFLALKSSIESLKEEGTQINLDYLKEPQKRLLKQYGLFVTKTYKTVILEKINKILRFIKQTKGGTSYISNDANKTGSDLLNDLKNFENNGT", "aa_hexdigest": "d5257342e868eee1ed4996e18030c368", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29618.561300000027, "isoelectric_point": 8.91064281463623 }, "id": "EFPKPENHBP_2025", "locus": "GCA000186005_02023" }, { "type": "cds", "sequence": "contig_1", "start": 1108385, "stop": 1108888, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTTTAAGTCCAAAATCTCAATTAATTTATGATGTTTTAAATCATGGAAAAATTGATTATTTAGCTAATGCAATTGAAAAATATTTATTAAATTTTGATTTAGTTAAACAACAATTTATTACACCTGAGCAAGTTTATCAATTAACAAAAGTTATGAAAAAAGTGTTAAATGATAACAACTTTGCTTCAGTGTTTGCTACTGGCAAAATGAACAAGAAAATATTACCTAAATTAATGTTTGACTTTGTTTATGAGTTAAGTCATTATGAAGGTGGTGATGTTCTCAAATCACTATTCTTTAGCATTCTAGAACAAGCAAAAGCTAAAATAGCTTCTAAATCAACTTTAGTTGAACAAAAAGCTTATTTAGCAGCTGAAGTAAATAACTTATACAAATTACTACAAGATTTAGCTTCTATTGATTTAAACCAATACTTAAGTGCAGAATCATTAGTTAATGTTTCAAATGATCCAAAAATATTTATTAATGCAATTTGA", "aa": "MLFLSPKSQLIYDVLNHGKIDYLANAIEKYLLNFDLVKQQFITPEQVYQLTKVMKKVLNDNNFASVFATGKMNKKILPKLMFDFVYELSHYEGGDVLKSLFFSILEQAKAKIASKSTLVEQKAYLAAEVNNLYKLLQDLASIDLNQYLSAESLVNVSNDPKIFINAI", "aa_hexdigest": "ec3adc94fcbe2a614e966504f7d60dc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19022.938800000007, "isoelectric_point": 7.803462791442873 }, "id": "EFPKPENHBP_2026", "locus": "GCA000186005_02024" }, { "type": "cds", "sequence": "contig_1", "start": 1110443, "stop": 1111135, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1", "UniRef:UniRef90_UPI000FF4EEE1" ], "nt": "ATGGTTAATGGTTTTAATGATTATAAACATGTATCAAAATTGCAAGATATCAATGTTGACTTAAATTTACCAACACAAGGTAGTGCATATAATCAAGGTTACTACATTAATGGTGAAAAATCAGATGATTTCAAAGGGACAAATAAAGACTATACACCAATTGAATATCAAATTGATTATCAAAGTGTAAAAGCTTCTTCATTGCCCGATTCAACTAAAAAAGAATATGAAGACTTTACAAGAAATGTTTTATTTTTTGGCAATGATAAAAGAGAATATTTGCCATTTAATAAAATAGGTAATATTGACCCTTCGGTAAATAGTTATTACTTTAAAAAAGATGACTTATTAAATCTTTACTCAATTTATAAAGCTGAACAAAACATTGAAAATAATAAAAACCCAGACATTGTTTTTGATTTAAAAGACATAAATAATTTAACTTTTGAACTAAAACAAAAAAACCGTTTCTTGAATATTTATGAAAAGAAAGGCGATACTTATGAACAAGTTTTTCTTGAAAAGAAACTTGAAAGAACTAACATTGTTCATTTCGATAAAACCTATAAAATAGGTAATTTAATGAATTTAACTATCAACAATAATGAAATTTATGCAACTCAATTAAGTTCATTATTTATTAATACTGACACAAAGGAAATAACAGCAGACTATTCAAAAGGTAAAAGTTGA", "aa": "MVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNSYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNIVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKS", "aa_hexdigest": "597a5b5683f5512ce0c003bbf20d6f55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 1110143, "stop": 1111261, "observations": { "mutation": [ 1110244, 1110283, 1110445, 1111258 ], "stop": [ 1110244, 1110283, 1110445, 1111258 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "query_cov": 1.0, "subject_cov": 0.49356223175965663, "identity": 0.991, "score": 456.0, "evalue": 8.05e-159, "gene_start": 1, "gene_end": 230, "reference_start": 43, "reference_end": 272, "reference_sequence": "MWRLFKEVFKSLSKNKVIVAGLSILVFLTSAIFTLLSNVSSSMVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNNYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNTVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKSWINQEKGIEIEPKQLAALFNFEPASFDGSTPNGLLFKQKDKTTNLDKFIKYNSSWAEINENLNLQSDWNLEDVFATNPKAIKAQKKLTLNKNNKFTLPIEWIAKEENIVRFLRWNYYTSFVDKDKSKDKWLGAFKTFMEDLIESSKTNPENKKMLDKLTTFSYWRKSKKNIVTPYKSDKTLDLSKKLQKNLLFH" }, "paralog": false, "identity": 0.967741935483871, "score": 713.0, "evalue": 1.85e-262, "description": "Internal stop codon at 1110244, 1110283, 1110445, 1111258. Nonsense mutation around 1110244, 1110283, 1110445, 1111258.", "psc": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF4EEE1", "UniRef:UniRef50_UPI000FF4EEE1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 26928.64820000004, "isoelectric_point": 5.372160148620606 }, "id": "EFPKPENHBP_2027", "locus": "GCA000186005_02025" }, { "type": "cds", "sequence": "contig_1", "start": 1111469, "stop": 1113019, "strand": "-", "frame": 3, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "nt": "ATGAAAAAAAATATGAGTATGATGGGTGCAATTGGAGATGTAAAGAATCCTGCAAGAAAAGTCAAATCAGATAAAACATTTAAAGATGTTGCAGGTAACAAAGAAGCAATTGAAGAAATTGAAGAAATTGTTGATTATCTTAAAAATCCTAAAAAATATGCACTTGCTGGTGCTAGAATGCCACATGGTATTTTACTTGGAGGCCCTACAGGGACAGGTAAAACATTGTTAGCTAAAGCTACAGCAGGTGAAGCAAATGTACCTTTCTACTTTATTTCAGCTTCAAACTTTGTTGAAATGTATGTTGGTTTAGGTGCTAAAAGAGTTAGAACTGTTTTTGCAGAAGCTCGTAAAAATGCTCCAGCTATTATCTTTATTGATGAATTAGATGCTATTGGTCGTACCCGTGGAGCTGGTTTAGGCGGAGGTCATGATGAACGTGAACAAACACTTAACGAACTTCTTGTTGAAATGGATGGTATGACTCAAAATAATGGAATATTAATTTTTGCAGCAACTAACCGTACAGACGTTTTAGACCCTGCTTTAACTCGTCCAGGTCGTTTTGACAGAACAATTACAGTTGGATTACCTGATGTTAAAGAACGTGAAGAAATTTTAGAATTGCATGCTAAAGGTAAAAGATTATCACCTGAAATTAAACTTGATCAAGTTGCAAAAAGAACTCCAGGATTTTCTGGCGCTCAACTTGAAAATGTTATTAATGAAGCCTCATTATTAGCAGTTAGAGCTAACTCAGACGTTATTAGACGTGAAGATATTGATGAAGGTATCGATAGAGTTATGGCTGGACCTGCTAAGAAAAATAGAGTTATTACTAAAGAAGAACTTACAATGGTTGCTTACCACGAAGCCGGTCATGCTGTTGTTGGATTAAAAGTTAAAGGTGGAAACAAAGTTCAAAAAATTACCATTATTCCTCGTGGAAATGCCGGTGGTTACAACTTAATGACACCCGAAGAAGAAAAATATAATGCAACAAAACAAGAATTGATTGCAATGATTACTGGATTTATGGGTGGTCGTGCATCTGAAGAATTAATTTATGGAAAAGACAATGTTTCTACAGGCGCTAGTGATGATATTTCTAAAGCAACCAAAATTGCTCGTAGAATGGTAACTGAATTTGGTATGTCACAACTTGGCCCTATTCAATATGAAAAAGATGAAAGCTCTCCATTCTTAGGCCGTGATTATCTAAAAAGTGCTTCATTTAGTTCTCAAGTAGCCCACGAAATTGATCTTGAAGTTAGAAGAATAATGACAGAAGCAGAAAAGAATGCCGCTAAAATCATTAAAGAAAACAGACAACTTCTTGAATTAATCAAGACAGCTTTACTTGAAAATGAAACAATTGTTGCTGAAGAAATTGAATACATTGAAAAGAATATGAAACTTCCTCCAAAAAGAGAAGCTGACAAAATCAAAACCAAAGTTTATACTTTAGATGAGCTTATCAATGAACACAAAGATGACAAAAAATTAGAAGATTCAAAAGCTGAAACAAAAAAAGATAATTCTAAAAAATAA", "aa": "MKKNMSMMGAIGDVKNPARKVKSDKTFKDVAGNKEAIEEIEEIVDYLKNPKKYALAGARMPHGILLGGPTGTGKTLLAKATAGEANVPFYFISASNFVEMYVGLGAKRVRTVFAEARKNAPAIIFIDELDAIGRTRGAGLGGGHDEREQTLNELLVEMDGMTQNNGILIFAATNRTDVLDPALTRPGRFDRTITVGLPDVKEREEILELHAKGKRLSPEIKLDQVAKRTPGFSGAQLENVINEASLLAVRANSDVIRREDIDEGIDRVMAGPAKKNRVITKEELTMVAYHEAGHAVVGLKVKGGNKVQKITIIPRGNAGGYNLMTPEEEKYNATKQELIAMITGFMGGRASEELIYGKDNVSTGASDDISKATKIARRMVTEFGMSQLGPIQYEKDESSPFLGRDYLKSASFSSQVAHEIDLEVRRIMTEAEKNAAKIIKENRQLLELIKTALLENETIVAEEIEYIEKNMKLPPKREADKIKTKVYTLDELINEHKDDKKLEDSKAETKKDNSKK", "aa_hexdigest": "30c967a6eebb0786c7bc6dbd82f278d3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AS56", "query_cov": 0.9166666666666666, "subject_cov": 0.9594320486815415, "identity": 0.7440000000000001, "score": 694.0, "evalue": 2.57e-247, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A449AS56", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AS56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "EFPKPENHBP_2028", "locus": "GCA000186005_02026" }, { "type": "cds", "sequence": "contig_1", "start": 1113254, "stop": 1113562, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAAACCAAGCATACTGTCAATTATTTTAATTACAGTATTGTCATTCTTAGCTTTATCAATCGTTTCATATTATTTGTTTAAAAGATTTGGACCTAAGCCTCAAATTGTTGACTTAAATCAATTTATGAATGATTTAAAAGCTGCTTCAGCTAGTCAATCAGATGATGTTTATTTTGCAAGTATAGAGTTTGACTCGTATTATGGAACTGCAAAATATGTATATGCTGGTTTAGACTTAAAAGGTAACCGCATATTAGTTGAAAGAATTGCTCAAATTGGAAGATCTAATGCAGCATCATGA", "aa": "MQKKPSILSIILITVLSFLALSIVSYYLFKRFGPKPQIVDLNQFMNDLKAASASQSDDVYFASIEFDSYYGTAKYVYAGLDLKGNRILVERIAQIGRSNAAS", "aa_hexdigest": "e4fc9cd3f6dad3d53c507396bf652e80", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11379.032299999993, "isoelectric_point": 9.221702766418456 }, "id": "EFPKPENHBP_2029", "locus": "GCA000186005_02027" }, { "type": "cds", "sequence": "contig_1", "start": 1114146, "stop": 1114511, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATTTTAGGAATTAGTGGCGGCCCAGATTCAATGCTACTTTTACATGAATATGCTTATAAAAAAGAGCCCATTTTTGTAGTGTGCATTAATTATAATCAAAGGATGGATTCACATAAAGATGTGGAAATTGTAGAAAATTATTGCAAACAAAATTTTATAGATTATCAAATATACAATTTTGACCAAAAAGACTATAAAACAGGCAATTTTGAAGAATGGGCCAGAGACAAAAGAATGGAAATCTTTATTCAAAATTATCATGAAAAGAATGCTTCTAAAATTTTGTTAGCACACCACAAAGATGATTTTTTAGAGTCATTTTTTATGCAAAAAGTAGCGAAAAGAAAACCTTCATTTTGA", "aa": "MYILGISGGPDSMLLLHEYAYKKEPIFVVCINYNQRMDSHKDVEIVENYCKQNFIDYQIYNFDQKDYKTGNFEEWARDKRMEIFIQNYHEKNASKILLAHHKDDFLESFFMQKVAKRKPSF", "aa_hexdigest": "515da2feb5bd3437c7b54be267890d98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14633.543999999987, "isoelectric_point": 6.506780052185058 }, "id": "EFPKPENHBP_2030", "locus": "GCA000186005_02028" }, { "type": "cds", "sequence": "contig_1", "start": 1114513, "stop": 1115073, "strand": "-", "frame": 1, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_013527204.1", "SO:0001217", "UniParc:UPI0001A8C3D9", "UniRef:UniRef100_C4XFM8", "UniRef:UniRef50_Q6KHA3", "UniRef:UniRef90_C4XFM8" ], "nt": "ATGAAATTAATCGTAGGTTTAGGTAATCCAGGAAACCAATATCGTTTTACAAAACACAATATTGGTTTTTTAACTGTCGACAAAATTTGTGCGAACTTTAATTTAACTCTTACAAAAGAAAAATTTAATGGCTTTTATGCAAAAACTGAAGATTTTATTTTAGCTAAGCCAACTACTTATATGAATAATTCTGGTGAATTTGTTCAAGCGATAGCTTCATTTTATAAAATAGATCCTTCAGATATTTTGATTATTCATGATGAAAAGGATTATGAAATTGGTCAAGCAGCAATTAAAGTAGGCGGTTCAAGTGCTGGACATAATGGTGTTCAAAGCATAATTAATGCTCTTAGAAGCAATGAATTTAAGAGGCTTAGAATAGGAATCGGAAGAGATCCTAAAATGGAATTAAAAGATTATGTTTTAAGTAATTTTACAAGTGATCAAATGTTAGTAGTCGAACAAGTTCTAAATGTTGCTGCACAAGCTGCAATATCTTTTGTTTACAATGATATTAAGATAGTAATGAATAACTTTAATGTTTTTCGTAAAGGTAAATAA", "aa": "MKLIVGLGNPGNQYRFTKHNIGFLTVDKICANFNLTLTKEKFNGFYAKTEDFILAKPTTYMNNSGEFVQAIASFYKIDPSDILIIHDEKDYEIGQAAIKVGGSSAGHNGVQSIINALRSNEFKRLRIGIGRDPKMELKDYVLSNFTSDQMLVVEQVLNVAAQAAISFVYNDIKIVMNNFNVFRKGK", "aa_hexdigest": "9457996ec59eb25c1fe2297bb449555a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C3D9", "ncbi_nrp_id": "WP_013527204.1", "uniref100_id": "UniRef100_C4XFM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3D9", "RefSeq:WP_013527204.1", "UniRef:UniRef100_C4XFM8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM8", "uniref90_id": "UniRef90_C4XFM8" }, "psc": { "uniref90_id": "UniRef90_C4XFM8", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q6KHA3", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHA3" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "EFPKPENHBP_2031", "locus": "GCA000186005_02029" }, { "type": "cds", "sequence": "contig_1", "start": 1115140, "stop": 1115313, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64", "UniRef:UniRef50_A0AB32XD64", "UniRef:UniRef90_A0AB32XD64", "PFAM:PF13538.12" ], "nt": "TTGAATTATTATAAAGTTCAAGGTTCTGAATCTGATGTAGTTATCATTTACTTTGACAAAAACATAGCCAAATATATTACTAAAAATCACTTATATACTGCTCTTTCAAGAGCTAAAAAGAAAATCATTATTATGAATGATGTAGATGAATTTAAAGAACAGTACCTAAACTAA", "aa": "MNYYKVQGSESDVVIIYFDKNIAKYITKNHLYTALSRAKKKIIIMNDVDEFKEQYLN", "aa_hexdigest": "8a3a66c0030187053a56bd7e3f5b5554", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B1", "uniref100_id": "UniRef100_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD64", "uniref90_id": "UniRef90_A0AB32XD64" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD64", "uniref50_id": "UniRef50_A0AB32XD64" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD64" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13538.12", "name": "UvrD_C_2", "length": 45, "aa_cov": 0.7368421052631579, "hmm_cov": 1.0, "evalue": 1.0999848812350724e-10, "score": 37.5797119140625, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 6808.7852, "isoelectric_point": 8.89220485687256 }, "id": "EFPKPENHBP_2032", "locus": "GCA000186005_02030" }, { "type": "cds", "sequence": "contig_1", "start": 1115595, "stop": 1115975, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355067.1", "SO:0001217", "UniParc:UPI0001E330B2", "UniRef:UniRef100_C4XFM6", "UniRef:UniRef50_C4XFM6", "UniRef:UniRef90_C4XFM6" ], "nt": "TTGTGTTCAATTAATTACAATTTTGATGATAAAAAAAAGATCATTAATTATTATTCTGAAGATTCACAAGTATTAAAACTTAAAGGCTCATTCCGTGCGAACAAAAATATTGAAGAATTCTATGAAAGTATTTTAGAAAACAAACCATTTCACTTTGATGGTGAAAATGTAATTGCATTTAATCAAAATGATATGGCTAGAAAATACGAAGCTCTCAAATATTATTATAATTATATTGACTCGAAGTTTATCGATTCATCAAAAAATATTAAAGAATGTTTCCCAAATTATAAAGATGAACTTTTTGTTATTTATGCACTTCTAAAAGAATTTTATGGGTGCAATTATCCAGAAGGAAAACCAACTGAGTTGAAAAAGTAG", "aa": "MCSINYNFDDKKKIINYYSEDSQVLKLKGSFRANKNIEEFYESILENKPFHFDGENVIAFNQNDMARKYEALKYYYNYIDSKFIDSSKNIKECFPNYKDELFVIYALLKEFYGCNYPEGKPTELKK", "aa_hexdigest": "de79ea82f2bb0fc4db5e2b84f9c6e752", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B2", "ncbi_nrp_id": "WP_013355067.1", "uniref100_id": "UniRef100_C4XFM6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B2", "RefSeq:WP_013355067.1", "UniRef:UniRef100_C4XFM6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM6", "uniref90_id": "UniRef90_C4XFM6" }, "psc": { "uniref90_id": "UniRef90_C4XFM6", "uniref50_id": "UniRef50_C4XFM6" }, "pscc": { "uniref50_id": "UniRef50_C4XFM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15124.932399999989, "isoelectric_point": 5.89718189239502 }, "id": "EFPKPENHBP_2033", "locus": "GCA000186005_02031" }, { "type": "cds", "sequence": "contig_1", "start": 1116001, "stop": 1116147, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5", "UniRef:UniRef90_C4XFM5" ], "nt": "ATGTTTATAACTAATACAAATGAACGAATGGTTTTAAACAAATTAAAAAATCACAACGTGAATGGTGTTTTAGCTGTTTTTGAATCTTTTGATATCAACAAAATGGTTTATAACAAAATAAAAGGTTATATTTCACATCAAATTTGA", "aa": "MFITNTNERMVLNKLKNHNVNGVLAVFESFDINKMVYNKIKGYISHQI", "aa_hexdigest": "e9c9e18933e5ad8ccf7977c8570a74e9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFM5", "query_cov": 1.0, "subject_cov": 0.8275862068965517, "identity": 1.0, "score": 98.2, "evalue": 3.61e-26, "valid": true, "uniref50_id": "UniRef50_C4XFM5" }, "pscc": { "uniref50_id": "UniRef50_C4XFM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.521000000001, "isoelectric_point": 9.60174388885498 }, "id": "EFPKPENHBP_2034", "locus": "GCA000186005_02032" }, { "type": "cds", "sequence": "contig_1", "start": 1116173, "stop": 1116328, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAAAAAACTTTTATATACAGCAGTTTCAAGAGCTAAAGAAAAATTATTTATTTTAGGCGACTTAGCTTTATACGCTAAAAAAACAAAAACTGAAGATTATAGTAAAATAAAAACATATTTATTTGATTTAATTAAGGAACAAAATTAA", "aa": "MLNKKLLYTAVSRAKEKLFILGDLALYAKKTKTEDYSKIKTYLFDLIKEQN", "aa_hexdigest": "6a4f5b27229a6941212a3248b91eafb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6000.0543, "isoelectric_point": 9.60180835723877 }, "id": "EFPKPENHBP_2035", "locus": "GCA000186005_02033" }, { "type": "cds", "sequence": "contig_1", "start": 1116689, "stop": 1116832, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATAAACTCAGTTGCATTATTAAACGAATTATCATACTTATATCAAACTTATAATCCAGAATACAATTCTGAAAAAATGGTAACCTTGATTCCACTTCATGATGGTCAAGTTGGAGCCAAAACAGTTAATAAATGA", "aa": "MKEINSVALLNELSYLYQTYNPEYNSEKMVTLIPLHDGQVGAKTVNK", "aa_hexdigest": "9eaa5eb767de2ec2b7712779c9ddf157", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.0606000000025, "isoelectric_point": 5.5737112045288075 }, "id": "EFPKPENHBP_2036", "locus": "GCA000186005_02034" }, { "type": "cds", "sequence": "contig_1", "start": 1116962, "stop": 1117555, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGCAAAAATTATATTATGCAGAAAAAGAAGTTCCAACTATAGCACTTAGTTTTATTAAAGAACAAGAAGACTTTATTTTTGAAAAATTAATAACTATAAAAAATAAGATAAATAAAACTATTCCGCAAATAGAAGATGATACATTAACTGCTAAACAACTTGATGCTTTAAACATTGCACTAAATGAACCGATAAGCATAATTTCAGGATTCCCTGGCACTGGCAAAACATATTTAATTAGTAATATTTATGAGTCATTAATTGCCCAAAAAGTCTATAAAAAAGGTGAAATAGAAATTTTGACCCCCACTGGTAGAGCTGCAATTAATATTAAAAATAAGAGTAATAATTTGCCTGCAAAAACGATTCACAACTATTTAAAAATTGATAAAGAAGATGAAGACGTTGTTTATAACAATGATTGTGACAAAACGAAAGTTGTAATTATTGACGAGTTTTCAATGGTTAATTTAAAGATTTTTTATCAACTTTTAAGTCATTTATCCAAGCTTGAAAAGCTCATTATTGTTGGAGATAAAGATCAGCTTCCATGTATTGGACCTGGCAATTTAATGCACGACTTATGTAATTGA", "aa": "MQKLYYAEKEVPTIALSFIKEQEDFIFEKLITIKNKINKTIPQIEDDTLTAKQLDALNIALNEPISIISGFPGTGKTYLISNIYESLIAQKVYKKGEIEILTPTGRAAINIKNKSNNLPAKTIHNYLKIDKEDEDVVYNNDCDKTKVVIIDEFSMVNLKIFYQLLSHLSKLEKLIIVGDKDQLPCIGPGNLMHDLCN", "aa_hexdigest": "a5d67d5ae5ef378a748e56494718cd55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 55, "aa_cov": 0.25888324873096447, "hmm_cov": 1.0, "evalue": 1.5822810310397043e-11, "score": 40.78759002685547, "start": 49, "stop": 99 }, { "id": "PF13245.12", "name": "AAA_19", "length": 138, "aa_cov": 0.6903553299492385, "hmm_cov": 0.9710144927536232, "evalue": 5.379265116549489e-34, "score": 113.59687042236328, "start": 53, "stop": 188 }, { "id": "PF13604.12", "name": "AAA_30", "length": 151, "aa_cov": 0.751269035532995, "hmm_cov": 0.9271523178807947, "evalue": 8.831164653815167e-26, "score": 87.11857604980469, "start": 49, "stop": 196 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 72, "aa_cov": 0.36548223350253806, "hmm_cov": 0.9722222222222222, "evalue": 8.577205669433242e-14, "score": 48.144962310791016, "start": 49, "stop": 120 } ], "seq_stats": { "molecular_weight": 22330.68700000001, "isoelectric_point": 5.688355445861816 }, "id": "EFPKPENHBP_2037", "locus": "GCA000186005_02035" }, { "type": "cds", "sequence": "contig_1", "start": 1117655, "stop": 1118245, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAGTGGGCCTAATAAATATAATAATTACATACTTGAAGGCTACAATATGATTGAAGCAACAAAAGAAGATTTAAAAGTTATGTTGAAGAATCATATTAGAGGTTTTAAAGAAGAAGATCATAATATTTTAAAAGAAAATTTAGGTGAAAATTATTTAGATGTTATTAAAGATACACCTAAAGAAGAAAGAAAAATATTTTACGAATTTATTAAACCCCCTTTGCTTAAAAAAATAGAAGCTTTTATTGAAGAAGTTCTAAACATGGGTAAAGATGAAAGATTTTTTATAACCAATAATATTTACAATTTTTATATTAAATTGAAGCAAAAATTACCTCTTGCAGATGGCGAATCATATTTAGATTTTTTTGATAAAAACAAAGAAAATCATGAAGATCCTTATCTTATTTTGTATATTGAATTAGGATTAAGCTTTGACTCAGTAGACAATTTTGCAAGCTTAATAGGATATTCAAAAGATTGCGAAACTAGATATTATGCAATAGTTTATAAATCTATTTATGATTTTGGAATTCAAGTTAATGATACATATTACAATTTGTCTGATTTACATAAAAATTGA", "aa": "MELSGPNKYNNYILEGYNMIEATKEDLKVMLKNHIRGFKEEDHNILKENLGENYLDVIKDTPKEERKIFYEFIKPPLLKKIEAFIEEVLNMGKDERFFITNNIYNFYIKLKQKLPLADGESYLDFFDKNKENHEDPYLILYIELGLSFDSVDNFASLIGYSKDCETRYYAIVYKSIYDFGIQVNDTYYNLSDLHKN", "aa_hexdigest": "9d758875175af236a0b93ed7cb931a44", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23331.19940000001, "isoelectric_point": 4.96354465484619 }, "id": "EFPKPENHBP_2038", "locus": "GCA000186005_02036" }, { "type": "cds", "sequence": "contig_1", "start": 1118626, "stop": 1118751, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDF1", "UniRef:UniRef90_A0AB32XDF1" ], "nt": "GTGTTTCAAATTTTATCAACTTTTATAGCTACAAATATGTTACACCACTATAATTCAGCAATTAGATTTAAATTTAGTTTCAAAAGAAAAGAAGATTCAATTTCTAAAAACTATATAAAAATTTAG", "aa": "VFQILSTFIATNMLHHYNSAIRFKFSFKRKEDSISKNYIKI", "aa_hexdigest": "492a51a002d70c947a0d391420f221db", "start_type": "Edge", "rbs_motif": null, "truncated": "5-prime", "psc": { "uniref90_id": "UniRef90_A0AB32XDF1", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.976, "score": 81.6, "evalue": 6.67e-20, "valid": true, "uniref50_id": "UniRef50_A0AB32XDF1" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDF1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.7185, "isoelectric_point": 10.118909263610838 }, "id": "EFPKPENHBP_2039", "locus": "GCA000186005_02037" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmopsis fermentans] [strain=M64] [gcode=11] [completeness=complete] [topology=circular]", "nt": "AACATTATTGAGCTCATTTAAATAAGTTTAATATTCATTTTCATTAACTATTTTTTTGTCTTTAAATAAGGATATTATCAAATTTTTAAGTATATATAAAGAGTATTCTTATTTTTAAACACAGTTTTCCACATAGTTATAAACATATGTTAATAAGTTAACTTTTCTACTTTTTATACTATAAATAAGACTTTTAATTGTTGATAAGTTTGTGGATAACTTGTTTAAATAACTAAAAAACTTACTAATTATTTCTTACATTAAAATATAAATAATACTATTTTTATATTATGGCAGAGAATAAAAATATAAAAGATAGTATCACAATGAAAGCAAATACATTAACATTTTTAAAATCTTTAAGTTCAGATGTTAATGACCAAATGTTGTTCAAAAATTTCATTTCAAACTTCACAATTGTAGGCATAGATCAGAACAATCATGTCAAAATTCAAACATGACTACAAAAACCAAGCTTAGAAATATTAATAAAAATGCTAGATGGTAATATTTTAAAAGCTTTAGAAGAAACTTTTGAAAGAGAATGCACATATGAATTTGTTCATATTCCTAAACCTATTGTTAAATTAACAAAAGCCTCAAGCAACAAAACAATTTCTTCTAAAACAATTGAAGAAGAAGTGAAATTAGGCGTTGATTTAAATAAAGAATTAACATTTGACAACTATGTTGAAAGTGATTTTAATAAAGATGCAATCAAATTAGCTAACTATTTAGTTAAAGGTGGAAAAGAATATAATCCTATTTTTATTTATGCTAAAAGTGGACTAGGAAAAACACACTTATTACATGCAATTGGTAACAAATTACAAGAGCAAGGCAAAGTTGTTAAATACATTAATCCAAGCATTTATGCTAGAGATATATCATTCTATTTGCAAGAAAATGATCAAGCAAAATTAAAGAAAATAAAAGATGAATTTGATAATGCTGATTACTTAATATTTGATGATTTTCAAAGTTATGGAGTCGGAAATAAAAAAGCAACAATTCAAACAATTTATAATATTTTAGACTATCGTTTGAACACAAATAAAACAACCATATTTTGTTCGGATAAGCCTATAAATAACTTGAATGCGATGTTTGATGAAAGATTAATTTCTCGTCTTTATATGGGTTTACAACTTGAAATTAAAATTCCACAACAAAGTGATTTATTAAGAGTTTTAGATTACATGCTAGATATTAATGATATGAATCCAGAGGCTTGAGAAAAAGAAGCTAAAAACTTCGTTACAAGAAATTACTCAACATCAATTCGAACATTAATTGGAGCCATAACAAGATTAAAATTCTACAAAATGGAAATGGCCCGGACTAATTCTAAATATACTGTTGCTGTTGTTAATTCAATATTAAAAGACATGCAACAAACAAAAGAAAATATTACACCAGATTCAATAATAGAATATGTAGCAAAATATTACAAAATTCCTAAAAAAGAAATCTTAGGCAAAAGCAGACAAAAAGATGTCGTTCTAGCAAGACACATAGCTATTTATGTTATAAGAACACAACTGGAAGTTCCATTAGAACAAATTGGTCATATATTTGGTAATAGAGATCACTCAACTATTATTAATGCAATTAGAAAAATAGAAACTGAAAGTGATATGCCAGATAAAACAATTAAAAGAACCATATCAATGATTAGTGATGATATCTACAAATTAAAATAGTTATTAACTACATATAAACATTAAAATTAATCAAAAATTTAAAAAATAAGCAAAAAAAACATAAAAAATAAACTTTTTAGAAGTTATCCACAAAATAACAATGCTTATTAATATTATATAAGGAGTTATTATGAAATTACACATTAAAAAACAAATTTTAGATGAAACATTGGAAATTGTTTCAAAGTATACAGATCCTATTAATTCATTGTATGGGTTCAGATGTATATTAATTAAAGTAGATAATGAAAAAGTAACATTTTCAGCATCTAATGGAGTTATAAGTATAGTTAAATCACTAGATGTTGAAGATGTGGATATTAAAGTAGAAGAAACAGGAACATTTTTAATTCAAGCAAATATATTTAAAGCTATTATTAAAAAATTAAGTGAAACAATTACTTTAAGTCAAGAATATTCAACAACATTAGAAATATCACAAGGCAATTCAAATTATTCATTAACTACAAACCCAATCAATTCATTTCCACAAATAGAAGAAAATATTAATTTAAAAAAAATTGAAATCGATACAAATGAATTTAAAAAAGCTATTAGATGCACAGCTTTTGCTGTTAGTCAAGACGCTTCATTAGTATATAAATGTATTAATTTTAAATTTAAAAATAATAAATTAAATCTAACAGCTACAGATGCAGCAAGACTTGCATATTACACAATGGATCTTAAAAGTTCGCAAGTAGATAATGAAGAATTTTCAGTAAACAATAAAGATGTTAAAGATTTAATACCTGCAGATGCATCTAAAAAAGTAACATTATTCTTTAATAGAATAAAAATAGGAATTGAATATAAAAATACAGTTATCACTTCAAGAATAGTTGATTTGCCTTACCCAGATGTTGAAAGTTTATTTGCTTCAATAGATATTGAAAATAAAATATTTATTAAAAAAGAAGAAATAAATGATTTAATTAACAAAGTTTGATTAGGAACAAGTGATAAACAAAATAGATTAGAATTTACAATTAATAAAAATGAAATAACTGTTTTAAATAGATTGAATGAAATCGGAACATCTATTGCAAAAACAGAAAATTTTAAATTTGAAGGAAAAGCTTTTGAATTTGATATTAACTATCATTTCTTAAAAGATGCTTTAAGTGTGTTTGAAGGTGACGTTTTAATTCTATTTGATAAAAGTGTACAAAAAATTTTAGTAGTTTCAGATTCAAATAAAGAATCTAAACAACTAGTAACTCCTATGAGAAGATAATATGAATAATGAAATTTATTTAAAAGATGATGTAATTACACTAGGTCAATTATTAAAGAAAATAGGACTTATTGACACAGGCGGATCAGCTAAAGGTTATTTACTTTCTAATAAAATTTTAATAAATAATAGAACACCAAATGGACGTAGTGCAAAAGTTAAAATTGGTGACATTGTGTGGATAAATAATAAAGTTTTTATGATTAAAAAACTTGAAAAATAATTAGATAATAGATATTTAATATCTTTTATTTTTTTATATATATTAAATCATTTAATAAATACTAAAATGTTTGTTTTATAGTTATTAAAGAAAAAATAAAAAGCGAGAAAATCTCGCTTAATATAATAATAAATTAATTAAATGTTACCTAATATTCATGTAAATACTTTATATTCATCATCGTCAACTGATGTTTTATATTCAAATTTAGTTAAATCAAATTTAACATGATCTCTAGCAACAATTCTAAATGATGCAGCTAATTGTGTATTACCATTTATTCTAAAAACTTTTATGTGGCGAGACATCGGTTGATCTTTATTATATTTATTACCAACAATATGATGTCTAATTAAAGTATCAAATTTTTCATCTTGAACTGTTTCAGTATCAGCTGAAACAAAGTTAAATACATATTCAAAATTGAAGTTGTTTTCCATTGTCATTTGGTTAAAACCATATTTTGTTAAGCCTTGTTTAATAGATGTTGTCATGCCAAATGGACCTGCAACATTCATTTCATTAATTGTGTTTTGATCTACTTGTTTGAACAAAATATTATTTTTTGCCAAGTAAACAATTAACTTCATTTTGTCTTTAAATATCATATTTTCAGTTGAAGATTTAACGTTTTTGTTAAAGCCACTGACTGAAATTGTTTTAGTATTTGAATTTGAATCAAACGAAATGTTTAATTCAATGTCTAATTTACCTTCAGCAACATTAGGTGTTGTTTTATTAATAACAACATTAACTGTTGAATTTATTTTTTTGAGATCATCTAAGTTTGTAATAGATACTTCATTTTTTGTAATATATGAAGACAAGAAGTATTTTTTGTCTAATTGAACATTTTCTTTAATTAATTGGATATTTAATAGCTTAACAATTTTTTCAGCTGTTAAAGTTTCTTTTTTGAAATCATTAATGATGTATTCTTTATTAATAACTTCAGTTTCTTTTGTGAATTTTAAGTTGAATTTAATAATACCTTCTAAATTTTTAACTTCAACAATAGTTGTTTCAAGAGAAACACCTTTAGTTGCAAAATCATAAGCAGTACTTGTTCTTTTGTCTGTGATTGTAATGTTGTTTACATCTGTGAATTCTTTAGCTTCTTTAGCTTTAAATGAATCAGCAATTGTAACTTCCATGTTAGTGATTTCTTCTGCTAATTCGCTACGGTAGAAACCTGAAATTGTAATTGGAACTTTATTAGAGAAAACATCTGGATGTTGAGAATTACTTAATTGAACATTAAATGTAATTTTGTCATTTAAAGTTGTGAAGTCTGAAATTTTAACGCTGTAACCTGAGCCAAAATCTGGGCTAACAATGTTTTCTTGAATGGCATTTTTAGCTTTTGTTGTTGTTTTGTTTTTATAATCAAATTCATTAATTTTTGAATTTACCATTGCTGTCAATTCAGCAGGTGTTTGGATTTTAGCTATAGTAGTGCTACTTTCTTCATAGTGTCTTACATATTTGCCATATACTTTTCCACCTAAGTAGAAACTTAAATAATAGTTTTCATTATCTTTATTTAGTAATACTTTTTTAGCTGAAGTATCATTATCTTCAGAAAATAGATATGCAGTAAAATTGTAACTAAATAATTCAACCCCATCTATTGAAATAGAATTGTTAAATGCATCTAAAGTTGTACCATCAACTAAGCTTTCTAATTTATTTAAAGCTTCAGTTGTTTCAACAAGTTTAATTTTATTAAGTTTTTTTAATGATGTAATTATTTCATTCGTTGTTGGTCTTACTTGAAGTTCTTGTTCACTTCTGAAGAATTTGTTTCCTTTATAAACAAAACTAAAATCAATTTTTCTTTTACCACTTTTGTATTCAGTAGAGCTAGGGCTAGAATTTGTGAATTTAAAAACAAAATCTGAAAAATCTGATGGTGTTTTAAGTCCTTCTATTTTAATTTTGCTTTCAAAAATATCAAAAGCATTTACTTTGTTAGTACCGCCTTCATATGATAATTTAGCTTTTTCAAATTCTTGAACTAAAGCATCATAATGATCACTTTTGTGATATATGTATTCAGTTCTAGTATTAGATTTATTGTTTTCATTGTGAATATTTGTAAGATGAAATTTAATTTCTAACTGATCAGCATTTTCTTTATATACTTCAACAGATTCAAAAACAATTTTTACTGTTGATGGTAAGTTATTAATAAATATTGTTTTGTATGTTGTTGTATTTGGATTTAAAAGTTCAGTTTTTGTTTCTTTTAAAGTTTTAGATCCATCTGAATAAATTTCAATATTTCCGAGTTGATAATTTAAATATTTGTCTGTTAATTTAAAACCATCAATTTGTATAGTTCTTTTATTTGTAGAAACTTCATTTCTAATTTTGTTTTTAATTTTAAATTCAAATTTTAATGTTGAATAAAATTTACTCAAAAGTTTAGATTCAACTATTTCAAAGGTGTTTTGGTCAAAATTAGAGTAAACAATTTTATTTATATTAGCATCTGTAACAAACACATTTAAGTGATCTGGATATTCAGCTTTAATTTGATTCAATACCATATCAGGTGTTAATAAAGTTTTTATTTCAAATGTTTTTTCTTTAGAGAATTTATTTGAATCTTTTTTATCAACAACTTTAAGTGTTAATTCAACTTCACCTTTATTTGCACCTTTTTTAATTGATACAATTTTAAAATTAACTTTAGTATCTACATCTGTAAATTTAAAAATGTCTTTGCCTTTAGCTATAACTTCATCAGCAGTTAAATTTTTATCTAATAAAGTTACTTTGATTTTTTCTAATAAACTATTTAGTTCTGTTTCAGTAAGAGATTTTGGAGTTTCCTTAGGATCTTTATGACATGAAATAGTAGTCAACGGTAAAGTTGTTACTGTTGCAATTGCAGGAACAATAGCCAAACTTATTTTCTTTATTTTTTTCATATTTTTCCTTTCGTTTTTTTATTTTGTCAGCCCTTTAATTATACAAAATTGACTAAAAATGCAAATTTTCTATTTTTCTATAAAAATAGATTAACAAATAAAAAACTATTTTTATTAAGGTGAATATTCAGATTATCAAAGCGTTTTATTTGTTTTTTGAGAACTATAAATTTTTCCATTTTGAGTTTCTAGTCTATATTTAATAATTACAAATTTGTGCATTTGTAATTCATTTTTTTCTTTGTTTTGTGAATTTAAAAGCACTTAGTAGTATTTAAACTATTATTTAAAATTTTAATAATGCCATCAATTTTTCCTTTGTCAAATGTTATAGCATTACTAATTTCTAAAGAAGCTAATTATTATTTTTAAATTTAGATTCATAATCAAATTTTTAGTTTTCTAAAGATCTTTCTATGTCTTCATTTGACTTTTGAAATTTGCCTAAATGTTTATAAGTTTTAGTATTTATTTTTTTATTTTGGTGCCACCAGATATATTGTAAAGTAATTCTCAACAGAATCTTTGTAAATCATTTTTAATTTCCAAATCGACTGTTTTATAGTATTTTAGTTAAAATCATAAGGCAAAATAATTTTAACATTCTGTAGTTAATTTTTCAACATAGTCTTTTCAAAATTGAAATTTTTAACTTCAAATTTAAGTTTACTATTTAATTCATTTAGTTTTTCTTTGATTTGATCATCTGTGTAATAGTCAGGTAATTTATCTTTGGTGTTTTTTTAAAACCACCATTGTATACTTTTGTAAATTTCATGACTTGATCTAAAATTAGAGTGAGAATTGATACCTCAAAAATACTAATTAATTTTTACAATAACATTTGTACTATCATTGTTTCCAATTTAAAACCTTGAAAATAATCTTTTAAAATTTCTCCGAAACTGCCAATTCTAAGTTATCTTTAATGAATTGTTCTTTTTTATAATTTGTGATTTTTTTGTATTTTTTAATAGTCATATTTTTTAATGATACTTTTTCGCCATCACTATAAACATATTTAAGAGAAAAAACAATATAAACAAAATTTGCTTTTTCATAATATTGGACGCTTTGTGTATAACTTGTTTGACATAGCATATGAATTAGGAATGATTTATAAATTTATCTAGATAAGGATTATTATCAAGTTTAAATTTAATTTTTCTATTGCTACTTCATTTACATATTTATCTTTGATTGCTTTGTTATCAGCTAGATAAAGTATTATTTTGATTTGACTTGCTATTTTAATAATTTTATTATTAGTTAAATTAAAAATTAATTCAAAACAAAAAGAGCCAATAAATAAGCTCTTAATGTGTTAATTTTTGTATTTGTTTTTACCCACTTGAACTTCAACATTTGTATCTATTTCAATTTTTCTTGTTTTAATATTGCTTGCATTTAAAATGTGCCTTGCTATTTCTGCGTCTTCAGTGTCATGATATTTGTCATCTTCATAAACTACTTCACTAATGCCTGATTGAGCTATGGTCTTAGCACAACTTGAGCATGGATATAAAGAAGTGAAGATTGTGCAACCTTGAAGATTATTGTTTGTATTTAGAATTGCATTCATTTCGGCATGAACAACATAAGCATATTTAGTTTCTTTTGGACTTTCACCTTCACGGTCTCAAGGGAATTCGTCATCTATGCCTTTTGGCATTCCATTGTAACCTAGAGCAATAATTCTTTTGTTTTCATTAACTACGCAAGCACCGACCTTTGTTGAAGGATCTTTAGATCTTAATGCAGATACTTTAGCTAAAGCCATAAAATATCCGTCTCAATATAATGATTTGTTTTTCATGCAAGAATTATACTTTTAAAAGTAAAAAAGCAAAATTTACTGCAAAAATGGAAAAATAAAACTTAAATCGTGAAATTTAAGCATTATAATAAATCTTTAATTTTGTCCATTTTTTCTCAGAATATTTGCTTTGTTTCTTTCGGGCATTCAACAGCAACATTTGAAAAAGCTTCAGGACCTGTGTGAATTGCAACAACAGGAGCAAGTCAGTTAACTCAAGGGTCTTGGTGGTATTGTTTTTTGAATTCTTCAATAAAAGTTTTTTGTTCTTCTTCTGAAGCACCTGTGTGTATATAAGCAGTAAGAAGTACATTTTTCTTGTCATTGATTTTGAAGTTTTTAGCTTTAGATACTATATTTTTAATAGTGGTTTTTGAAAAAACATGACCGACGCCTTCTTTTTTAAGTTGACCACCATCAACAGCAATTAAAGGAACAATTTTAAATAATTTTGCTATGGCTGCGGCAGCAGGATGCAGTCTTCCACCTTTAACTAAATAATCATTGTATTTAGGTATAAGGGTAATTGATTTGTGGAACCCCCCGTCTTCAATGTATTTAAGATATTCATCCATTTTTGAAGGATCTTTAGCCATTTGTTGTTCGAATCAAATTAAATCTAAAACAATTAATTGAACAATTTGAACCGATTTAATTGTTCTTAATTTTGGAAACTCTTCTTCCATTGTTTTTGAAGTTTGATAAGTACTTGACAAATGATGAGAGATTGGATAGAAAATAATTTTGTCATACTCTTTTGACAATTTTTCAAATAATTCTGTAGTTTTAGCTATATTGATAGCCGATGTTTTTACTTTAGCTTTTTTAGTGTAAAAGTTAAACAAATTAGAGGTGTTGATATCAACACCATCTTCAAATTCCTTCTCATCGATATTTATATGTAGAGGGGTATAAAATCATCCTAGTGCTTCAGCTTGTTTTTTTGTTAAAGCACATGATGAGTCGACAATTATTGCATATTTCATATTTTATATTATATAATAAAAATATTATGGGTATATTTTTTAATTTAAATTCAAAATTTAAGAACACAAAAATCATATATTTTGATACAACAATAAACAAAAAAACTATGGATATTTATGAAGATATGGTTGTTTTTAGAGATGAAAATTTTAATGTAAATTCGGTTAATTTACTGAATGCAAAAAGTAAAAAAAGAGATGAAAAAATATTTGGTTTTTTAAATGAAAATGAAACTAAAAAACTAGTCAATAAATTGCACAAATATAACAAAAAATATTTACTAAAATCAATTAAATACTTTAGAATTGGTAAAATTGTAGAAAGAAAAAAACACCCAAAAAGCGATAGATTATTTGTATTAAAAATAGATTTTGGAAATGAAGTAAAACAAATAATAACTAACACAACATATACACTTGAAAATAAATATTTTACATTCTGTTTACCAGGATCAATTACAGCTAATGGATTAGAAATTTTAGATGGTAAAATTATGAATGTAGAAAGTGATGGAATGCTTTGCTCAGCAGAATCTTTAGATTTAGAAGCAAAAAGCTATAAAGAATTTGAAGAAGGCTTTTTCAATAATGTTTTAGAAGAAGAAAAACAAAAATATTTAGGCAAAGAAATACAAGAATTATTGCCAGAATTAATAAGCAAATAGGAGTAATCATGACAATATTAGAAGATTTAAAATTAAGAGGAATATTAAAGCAAATTAGTAATGAAGATAAGTTTAAAAAACTTGATCCTAAGAAAGTTGCTGTATATTGTGGTTTTGATCCAACTGCGCAAAGTTTGCATTTAGGAAACTATGTTTTAATTTCAGTTTTAAAAAGATTTAAGCAATATGGTTTTAAAGTTTATGGACTTATTGGAGGGGCGACAGGAATGATTGGCGACCCTTCATTTAAAGATAGTGAAAGAGTTCTTTTAGATAATAAAACTTTGAAATTAAATAAAAGTAAAATTAGAAAACAATTAGAAGCTCAAGGCTTAGAAGTAATTGACAACATTCAATTTTATGAAAAAATGAATGTGCTTGAATTCTTAAGAGATGCCGGGAAACTTGTTAATGTTTCATATATGATGACTAAGGATTCAGTTCAAAAAAGAATTGAAAGAGGTTTGTCATTTACTGAATTTTCATATCAAGTTTTACAAGGTTGAGACTTCTTGCAACTTTATAAAAATTACAATATAATGATTCAAGTTGGTGGTTCAGATCAATGAGGTAATTTAACTACAGGTCTTGAAATGATTTCTAAAGTTGAGGGTGATGATCATAAAGCTGTTGTAGTTACTGCAGACTTATTAACTGATGAAAATGGAAATAAAATTGGTAAATCAACAGGTGGTGGCAAACTTTGATTAGATAAAACTATGGCTTCACCATATGAAATGTATCAATACTTATTAAATCAACCAGATAGCAAAGTTGAAACATTTTTAAATTGATTAACATTTTTAGATAATAAAAAAATAGCAAAAATTATGGAAGAACATAATCAAGCACCGTTTAAACATGTAGCTCAAAGAGTAGTAGCTAGTGAAGTAGTTAGAGATATTTTTGGTCAAGATGAATTAGATTCAGCTATTCAAATTACAGAAGTTTTGTTTAACAAAAATTTTGATACAAGCAAACTAAATTTAAAAGATCTTAAATCAATTGAGCAATATTTACCAGTAGTTGAAATTAAGCAAAATCAAAATATTATTGAAACTTTAATTGAAAAAGGTTTTATTGCTTCAAAACGTGAAGCTAGAGAATTTATGGCAACTAAAGCTTTAAAAATAGATGAAGATATAGTTGAAGAAAACTCAGTTTATAAACCAAAAAACTTTTCTAAAAAGTATGCATTCTTTAGAAAAGGAAAGAAACAAACTATACTATTAAAAACTATTTAGTTGATTAAAAAAAGCGGAAAAGACCGCTTTTTTAATTTTGATTACATAAATGTTTCAACTAAAGGAATAATGCCAACTAGTAAACCTGCTGCAAAAGGTGTAGCACCAGGAACAATTGAACCATATACTCAATTTGCTGAAGGAGCTTTAACTACTTCTCCTGTTTTTTCATCTTTTATTCCGTCTTTAAAGAAGAATAATACTGTCATTTGATAAACAAGTCTTGGACTTCAGTCTCTTGCAGGATTAAGTGCAAAACCTGTTACACCACCAAGTGACATACCAATAGACATAATAACTAATGCCACAGGTAGAGGGTCTAAAATAGTAAATTTTGATTTTTCTATTAAGTAAATCATAAGAATTAAAACAGTTGTGCCAACAAATTCATAAAGAACATTTTGTACTCAAGCGTTTTCATGTGAAGCACCTGTGGCACTTGAAGCTTTAAGAAGGGTTAAATCATTTTCTTTAATGTGTTTTCAGTTCATTACATTTAATAAAATTTGAGCAAGTAAGGCACCAACAAATTGCATTATGATCATTAAAATACCATTACCTCACGCCTCACCTGTTGTGTGTTTTCCAAATGAAATTAATCAATATAATGTTACTGCAGGATTCAAGTGACCTAGTCCACCAATTCCAATAGCAGCTAAAGCACCAACAAAAATTGCTAAACCTCAACCTATTGCAATTAATAATCAACCTGCACCTTTACTATGCATATTTTTAAAATTGTTTGCTGCACAAACGCCGTTACCTAGCAGTATTAATAAGAAAGTACCAACTAGTTCTCCAACAATTAATTGTCAACTCATAATATCTCCATTTTTTAATTTAGTGTGCTTCTTTGCACAAAAAAATTATATTGCTTAAATTAATAAAAGTTATCGATATGAAAATATGAATAAAGGTATTATTTACTATTATTTTGTATAATTTATAATAATTTATATGTGCCAATTTCGCTTAGTGGTAAAGCAGCTGACTCGTAATTAGCCGCCATGAGTTCGATTCTCATAATTGGCACCAGATCATTTTAAGTAGCTCAAAAAGCTACTTTTTTGATAAGCATTTTTTCAAAAACGACTGTTTTATAGAATTATTAGTCAAAAATGTATAAAAAAGATTATTCTATTTTTGTAGATTAAGTCTTAATAGTTTTAAATTGTTTCATTAATTTTTTGGAATTCGTTTTTGTTGTTTTCAGGTAAAGATTTTTTCAATTTAATATTATTTCACGTGTAAATAAAATTACATAAAACAATAGATATTAATGCAAAAATAGTCATGGTTATTAAATACTTAGAATATTCATTTCAAGTTCTTTTAGGTTTAAATGTAAATGTTACAAATAATATTGTGAAGAGAATTGATAAATCAAATTGGATTGCTGTTGTTATAAAAGCAGTTGATTTGTATTCTTTTTTCATAATACTTTTAATAAATAAATTAAATAACCTTAAGCTGCAATAAGCATAATATCTATAACAAATTTAAAAATCAAACCTAATGAAACTCCCACAAATATTATGTCAATATTTAAGTTTTCCATTCTATCCGCCAAGAAAAACAAAAATAAATGAAAAAATAATTGATAAAATACCTAAAATTATTCTTTCATCTTGATATTTGTTAGCGAATTTTGTTATTCTAGTTGTTGCATTTGTTTTTAAATCTCAATGATCAATAGTTTTTATAAACATAGGAATAAATTTGCACATATTGATAAAAATATAATAGTTAATATACTTAGCAATGTTGTCGATATAATTTCTCAAACGAAAACAATTTTTGAATTGTAAAGAAGAGTTTATTTTTCTTCTTTTAATTAAGTATTATTCATTTACCTTCTTATTATCCCTTTTTGATTTTAATAATTAAAATTAATTTTTAAAAGAAAATATTTATGAAAAACTTGATGTTGGTTTATATTAAACATTTTTTGACTTTTGAATACAAAAAATGTTTCATCTATGCCTACATATTTAAAAAATGTTTGAAAATGGAGCACTCATAAAAATAAAAAATTTTGGCTAAAAATATTTTTATATAAAATATAATAATTAAGAAAAAGAGGATTAATGAAAAAACATATTGGATTAAGAAAAGAAGCTAAATATGAAGCAGATTTTGATGATAAAAAAATTAAAGCTTATATTGACTATTTAATTTCTTGAATGAAAGAGAGAGTTGCTGATGCTTGTGCTAAAGGAATTGTTTTAGGTATTAGTGGAGGTATAGATTCTGCTGTAGTTGTCGCCTTAGCAAAAAAAGCTTTTGGCAAAAATGTTCTAGGTGTAATAATGCCTGTTGATAGCATGCAAAATGATTTAAAAGATATCAAAGAATTAGAAAAAGCAGTTGATTTAAAATTTATAACTATTGATCTAAAAAATACCTATGATACTATAAAGCAGGCTTTACCGTTAATTAACAGCAATTTAGCTTTAAGTAATATTAAACCAAGATTGCGTATGGCTGTCATATATGCTTTAGCTCAAGAAAACAATTATTTAGTTGCAGGAACAGGTAATAAATGTGAAACTCATATTGGTTATTTTACAAAATATGGTGATGGCGGAAGTGACATTTTGCCTTTAAGTAAACTGTTAAAAAGTGAAGTAAAAATTATTGCAAAGCACTTAAAAGTGCCAGACTCAATTATTAATAAAAAACCTTCTGCCGGTCTTTGAGAAGGACAAAATGATGAAGATGAATTAGGATTCAGTTATAAAGATTTGGATTCTTATTTAATGGATTCTAATGCAAAAATTAGTCAAGAGGTCAAAAATAAAATAGACAAAATGTACAAAAATAGTGAACACAAAAGAAATGTTTCATTACAACCTCAAGAAATTGATACAATATTAAAATAAAAAAATCACAAGGAGATTTATTATGTCAGGACACTCACATTGAGCAGGAATTAAACACAAGAAGGGAGCAAACGATGCTGCCCGTGGTAAGATATTCCAAAAAATGTTTAAAGAAATTTATGTAGCAGCAACAACAGCTGGTGGACCAGATCCAGATATGAACCCAGCACTTAAATTAGCTATTGCAAAAGCTAAAAGTAAAAACATGCCTAAAGCAAATATTGAAAGAGCTTTAGCAAAAGCAAAAGGTGATGGTAAAGATGCATCAGCTTATACAGAAGTTTTATTCAATGCAACAATTGCAGGGGGAGCTACATTTTTAGTTGTTGCCTTAACAGATAATATGAATAGAACAACAAGTTCTATGAAAGCACACTTTAATAGACAAAATGCAGCTTTAGGTAAAACAGGTCAAGTACCTTTTGCTTTCGATCATAAAGGTGTTTTAGAAGTTTCAAAAAACTTAGTTGGAGAAGACGATCTAACTATGGTTGCACTTGAAAATGGTGCCGAAGATTTTGAAGTAACTGAAGATTCTTATATAATTACAACAGCACCAGAAAACTTTTCAAAATGTAAAAGCGCTGTTGAAAGTAACTTAAATATTAGTGATTTTATGCAATGTGAAGTTACATATATTCCTAACACAACAGTTGCTTTTGATAAAGAAAAAAGTGAAAAACTTCAAGAATTTATCGCTCGTTTAGAAGATGACGATGATGTTCAAGAAGTATTTCACAATATTGAATTTGAAGAATAATTATTATCTTTAACTTCAAGTAGAAAGTTAGTATGAGAAAATTTATTAGAGCTCTTGAAAATGGTGCTTTAACAATAGGCACAAGAGAAATAGCAGCTAAATTCTATTTGATCTTAATTGCATTTGCAATTATATTTTTAGTGCCAGTTTTAGTGGCATTAATTTTTCCTTTATTTAAGAAAAGCTTAAGTAAAAAAGGAAGTATATTGCTTTATGCTTTTGTAACTGGTTTCTTTTTAACGATGGCTTTATTTGGATTTTTAAAAGAAGCTTTGGAAATAAGTTCAATTAGTGCTCCGGCATCAATGCCACCAGAAGCTTCGTCAGCGCAGATTAAATGAGCAACATATGGTTGAAACATATTCTTATTAGTTTCAGGTTTAGTTGGTGGATTATTATTTGCACTTGGAATTAGAATGACAGTTAAAAAGATAAGTGAAAAACATTTAGCTAAAAGTAAAGCAAGTGTATTTGTGCATACACATGATATGGTTCATAACCATGAACATGGTGAAAATGAAGAACATTCTCATGATCATGTGGCACAAGAACATAAAATATCAACAGACGAAAAAAATGCTAATAACAAACTAAAAGTAGTTGCATTATTGTTGTTATTAACTCACAGAATACCAGAAGGTTTCTTAATTGGTTATTCATTAAATAACTTATATAATCAGAATATTGGCTCATTAAGTATTGCATTCTTTGTTTCATTTATAATGCACTTAATACCTGAAGAAATGGTTTTTTATTATAGACAACGTGAAATGGGTGTAGGTCGTTGAAAAGCAATTGGAATATCAATAGGTTGTTTATTCTTATTCTTGCCTATGATGCTTTTAGGAATATTCACAGGGGATTATATTCAACAAATTTGACAACTAAGAGCATTCTTACAAGCAATGATAGCTGGTATATTCTTCTTTACAGCTGTGATGGAATTTCTACCTGAGTTTTATCATGCACATCATGACAAAGATTTGTTCAAAAAAGTTATGATTACATTTATGGCTGGACTTGTGGTTTGTGCCTTGATATTAGCATTGCATCAACATGGGACATATAATTAATTTAAGTACTTAGCGAAGTACTTTTATTTTTATTATTTTGCTGTTTTATAGTGTTTTTGGTAATTTTTCTGATACTATTTTTTCATTTTTCCCTCGAATAAAATTGTTTAAGATATGAAAAAAATAATTTAAAAAAATGAACTCTATTGAGTCCATTTTGTGGTCTAAATTATTTAATAAGACCTTCTCATTTTCAGTTTTTAACTTCAGGAATATCTGTACCAACTTCACGAATGTATTTGTTGTGGAATTGGATTGTTTCTTCCATTTTGTCCATGAATTCTTTTGCTTTTGATGATCCGAATACAGCTGCAGCAGCATCGCTAGCCATGTGGAATCTGTCCATGTGACTTAATTGACGAATGTCGAATGATGTTGTAATGTCACCATTTTCTCTATAACCGTGAGCAATTAAATTGTGGTTGTGACGGTCAAAGAAGATGTCTCTTAAAATACCTTCAAAACCATGGAAAGCAAATAATACTGGTTTGTCTTTTGTAAAGATTTCATCAAATTCTTCATCACTTAAACCGCGTGGGTCAATTTTTGGATGTCTTAATTTAAGTAAATCTACAACGTTAACAAATCTAATTTTCATACTTGGATATTCTTTGTTAAGAATTGAGATAGTAGCTAAAGCTTCTAAGTTAGGTTCTGTACCACTAGCAACAACAACAAGATCTGGAGTTTCATTTCTTGAACATGTTGAAGCTCAATCAATAATGTCTAAACCTTTTTCAACCAATGTTTCAGCTTCTTCTACTGAGTAGAATTGGTGTCTTGGTTGTTTTGAAGAAACGATTAAGTTAATTACATTTCTTTCTGTAAATGCTTTATCCATAACGGCTAATAATGTGTTTGTATCAGCTGGTAAGTATTCTCTAATTAGTTCTGGTCTTTTATCTGCCATGTGTCCAATAATACCTGGGTCTTGGTGTGTATAACCATTGTGGTCTTGTTGGAAAGCAGTTGAAGTAGCCATAATGTTTAATGATGGATAATCTTTTCTTCAGTTAATTTCTTTTGCTTTTCTTAATCATTTCATGTGTTGTGTAACCATTGAGTCAACAACTCTTAAGAATGATTCATATGAAGCAAAGATACCATGACGACCTGTTAATACATAACCTTCTAAGAAACCTTGAGCTTGGTGTTCTGAAAGTTGTGAGTCAATGATTCTACCTACTGGACTAAGAGCTTCATCTAATTTAGAATCAATTCTTTCCATTCATTGACGGTTTGTAACTTTGAATAAGTTGTAAAGACGGTTTGATTTAGTTTCATCTGGTCCAAAAATTCTAAAGTTTTCTGGATTTCTTTTTACTAAGTCAGCAAAGTATGTTCCTAATTCAACCATGTCTTGAGCAACAACTGAACCTGGTCTATCAAAGTGAAGAGCAAATTTTCTTCAGTCTCCAAGTTTCATAACTTTAGGATTAATACCACCGTTTGTAATAGGGTTCATGCCCATTCTTCTTTTGCCTTTAGGAGCAATAGCAGCGATTTCTTTTACCAATTTACCTTTTTTATCAAATAATTCTTCAGGACGATATGATCTTAATCATTTTTCAAGTGCATCTGCATGTTCCATGTTTTCTGCATTAATTGGAAGTGGAACTTGGTGTGCACGGAAGCTTCCTTCAATAGCTTCTTTGTTTCATGATTTAGGACCTGTTCAACCTTTAGGTGTTCTAAGAACGATCATTGGTCATACTGGTCTTTTAGCTTTGTCAGCTGGTTTTTTACGAGCTTTTGTTTGAATGCTTTGAATTTCTTCAATAACTGAGTCTAAAGTTTTTGCCATTAATTCATGCATAACTTCAGGTTTGTCGCCTTCAACAAAGTGAGGTTCTCAGCCCATACCTCTAAAGTATTGTTGTAGTTCTTCATTACTTTTACGGCTTAAAATTGTAGGGTTTGAAATTTTTCCACCGTTTAAGTGAAGAATAGGTAATACAGCACCATCATTTACTGGGTTAATAAATGAGTTTGAGAATCAGCTAGTTGCAAGAGGTCCTGTTTCAGCTTCACCATCACCAACGATAGTAGCAGCAATAACATCAGGGTTATCTAAAATTGCACCTGTTGCGTGTGAAATTGAGTAACCTAATTCTCCACCTTCATGAATTGATCCAGGGGTTTCAGGAGCAGCATGTGAAGCTGTTCCACCAGGGAATGAGAAGTATTTAAATAAATGTTGCATTCCGGCTTCATCTTGTGTTACATGTGGGAATAATTCTGTGTAACTTCCATCTAAGTATGAGTTAGATGTCATAACTTGGCCACCATGTCCTGGTCCTTCAATGTAGAACATGTTTAAGTCATATTTGTTAATAACTCTGTTTAAGTGTGCATAAGCAAAGTTTTGTCCAGAAATAGTTCCTCAGTGACCAATTGGGTAAACTTTAACATCATCAGAAGTTAAAGGATGTTTTAAAAGAGGATTATTTCTTAAGTAAATTTGACCAACTGAAAGATAGTTTGCAGCTCTTCATCAAGCATCAACTTTTTCAAGGTATTCTTTACTGTCGAATTCTTTTTTGTTCATTTGATCTCCTTATTTATTTTTTGCATTTGTTAGTCTTTCTAAAGTTTAGGCTAAATTAATAACCAGAAGAATAGAATAGACAAGTTTATACTCTCCCGCTTTTGTTACTTACAAATTGCAAAAAAATTATATATTAAATTTAAAATTTATATTGATAATAAATTATTATTATTTGATTTTTTTAAAAATAACAAAAAATCCTAGCAATGCTAGGATAGAAATAAGTAAAAATTGTATTAGAAACTGCTTTCGTATTCTTTGGTGTCAATATCAAAGTCATCTGAGAAATCGTATTCTTGAGTTTCGCTTTGTTTTTTGTTTGTTTCAACATCATCTTCTTGAAGCAAACCGAAGTAGAATACACTTGATGTATCAACTGAGCTTGATCTTGATGAAAAGTTTGTGGCTATTTGATAAAAGTGTCTAAAATCTTTTGAGTTAAAGAATTCTAAATCCTTTTCAGTAATTTGAGTTAAAGGCTCAAGCATGTATAGTTTGTCACTAACTGCAACATTAGCAGGTTTAGGAACAACCGAAATTTGGTTTGTGTTCATATAAAGAATATAAAGGTCTTTTCTTTCTAAAGTTTTAGCTAAATTAGTATTTGGTTTAATTAAATTTAGACTAACAAATAAGTCAGTGCCATTATTGTTTTTAACATATTTGTTTTTGTTATTTAAATTTAATGGTTGAATTAATCAAACCGAGTTACTTTCATTGTAGGTTGTTTGAGGAATTGAATTTTTCATGATATTTAATGAATTGAAATTCATTCTACGAGCTATTGAGTTAAAGAAAGTATTACGGTAAAGTATTCAGTTAGGATATGTTTTTGAAGTTAAGAATCTTTGATTAATTTCATGCAATTTTTCAAATTTAGCTGAATATACAACAGTTGTATTGCTCATTGAAACTTTTTTAGCTAAATTAGCAAAAATAATAATGTTTTCATTACTAATTCCGTTGTAACCAAGAGTATCTAAATCTACAATGTGATCAATTGTAAATTTTGAAATGTCATTTCTTAATTCTTCATATTCATTTGTTGATAAAAAGTTTTTAGTTAAAACTGTAACAATATATTTGGCTCATTTTTTACTTTGCATTAAGTATAATGGAGCAAAACTTTGTAATAATTTGTTTTCTTTAGTTAATGAAGGATATTTTTTTCTTAAAGCTGGATGAGACATTCTTATGTTAGGGAAATCACCAATTAATAAGTCAATTGGTTCATTAGTTCTTTTCATTAGAAAATCAGCATTTTGAAAACTAACACCAAAAGTAACTTTAAGTTTTATCATTTTAGATAAAGCGCGAGCAATATTTATTGTTTCATTATCTACATCATAAAAAGTAATACTTAGTTTGTCTTTGTCTCCATATTTTTCAATAATTAAAGGTAAGAAAATACCAACACCACATAAAGGATCAACAATCGTTAATTCTTTGCCTCTAATATTTGGAAGTCTTTTGTAAATTGAGTTTAGTAAACTTTCATCTGTATAAAAAGGTTTTTGACCTAATCTCAAGCAGTTTGCATATTGAGCTACTTGGTACATTGATCTAACATCCATAAGTTTTGGATTTTTTTCAATATATTTAATTAAATATTTAACATCTGTTAATTTTTCTTTTTTGATGATATCTTTTATGTTTCTTTCAGTTAGTGAACCAAACATATTTCCTCCTATTTTGTCTTTTTTACTATAAAATAAGCATTTATAATCAATTTTGTGAGTTAAATTATAATTTAATTATAATATATATTTTAGATTAATATTATTAATATTAAAAAAAGTGGTGTAAAAAATTTTATTAGGTAATTTTTGACAAATTTAACTATAAAACAGGACTTTTGGTAAAACGCAAAAACAATTTTAAAAAATATTGCATACAAAATTAATTTTTTCTATTAAAATTAAATATGCAAAAAATGTTTTTATATTATAATTACATTGCTTATGCAAATGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATTTTGCCCCATAGCCAAGCGGTAAGGCAACGGGTTTTGGTTCCGTCATGCGTTAGTTCGAATCTAACTGGGGCAACCATATCGCTGCGTAGGCGATTTTTTTATTTTTATTTTTTTACTTTATAATATATATGTTATGAATAATGATTATGAACAATGAACAAATAAAAGACGATAGCTTTAAAACAACTAACGATGTCTTTGTCAGAAGAAATAGTTTTTTAGGTTACACATTACTTTGATTTGCATTTTGCATGGCAATTAGTTTCGGTACAGCATTTGTATGTACATTGTATATGGAAAAAATTGCTGGCTATTTTGCAAATAATGTTATTGGATGAATAATTGGAGGTATTCTAACAATAATTTTACTTTTTGTATATATGTTTTTAGGACCTAGAATGCATATAGGTGTATCCATACCTATGGTTATGATCATAATGATGGGATTTGGTCTATTTGCGCTTTCTATTCCTCTTTACTATGCAAAAGAAACCATGGTTTGATGAAAATTATATTTAGTATTATTCTTGCCAGCAGGTTTTATGTTTATTATGGGAACTTTAGCAGCAACTAATAAAGTTGATTTAAGTAAAATTTGAATTCCTATTATAGTGGTAGTAGTAACAATGTTGATTTTAGGTATTGTATCATGATTTGTATTTTCAAGTTATATAGTTGCTTTAATTTCAGCATTAGGTGTTTTATTAATGGCACTTTACATGGGATTTGATTGATACTTTATTAGCAAATACAATAAAGTTTATAACAACTTTATGGATGATGAAGAAACCAAAAAAGAAATGATAAAATTATCTCTTTTCTTTGGTTTCAAATTAGCATATGATTATATTTATTGTGTACTTTATTTAATTAGATTATTGTCAAATATGAAAAATTAAAACTAGCCCCTTTTTAGTAAGCTAAGCTTATTTATTGAGCTAGTTTTTTTAATTATTTTTTAAGCTAAAGAAACTTCTGCATTGTAAACAATTGGATCATCCAAAACAGGTTTATTGTTTTCAAATTTAGCTAATTTAAAGCTGAATTTTACAGTATTGCCTTCTAGTTTAAAATCAAGAATTGAAGAAATAGATTCTCCTTTTTTGCTATTATATGTAGGTTTTTTTGCATTTGCAAGTTGTACACTATTTTTTTCAATATGTTCTTTTAGTGCACCTGTTATATTTAATGCAATAACCTTGTCTCCATTGCTTCAATTAATTCTTCCTTTAAAGCAAACAATGTTGTGGACTTTACGATCATATGCAAATGAATATGATTTAGAATCTTTTTTTATGGCTTCAATAGCTTCTTTAATTCTATCTTTGCCTTCTCCTAAAGTAAAGCATGTTTTATATTTGTCTTGGTCAGATGTACTTTTACTTTCAACCTTAGCAACAACTTTTTTAATTTTTGCATCTACAGCTTCAAAATCTTTAACTGTTTTAGCAGCATCGAAATCTTTTTTAATAGCTTCTGCTTCCTTTTTAAGTTCAGCTTTTTTAGTTTCATCAGTAACAGTTTCAATTTTAGCATTTAAATCTTTTAATGCTTGTTCAACTAATGCTTTCTTAGCAGCAACTGGATCAGTAGGGGTTTCTCCACCTTTAGAACCACATCCAGCACTAATTGCTACTGGTGCAAAAGATAAAATTCCTGCAATTGAGCCTAATTTTAAAAATTTTTTATTCATAGATTTTCTCCTCATTATTTTTACTAAATAATTATACTATTTTTTATATATAAAAATTAAAAGTACCACATAAATAAAAAAATGGCGTCCTTGAGTGGAATTGAACCACCCACCCCAAGCTTAGGAGGCTCGTGCTCTGATCCATCTGAGCTACAAGGACATATTTGTATTATATAACAAAAAATAAAAATAAAAAAATGGCGATCACGAGAGGATTCGAACCTCTGACCACGGGCTTAGAAGGCTCGTGCTCTATCCTACTGAGCTACGCGACCACGCTATTTACAAAACATTTATTTTTGCATTTATATATTACCACAATATTATTTTTTTTATTATTTTTTGTTTTAAAAAAATGATAGGGTCTAGTTTTAAAGAAATAGTTAATTTTACCAAAACAACTATCAAACAATGATATTGGTTAAAAATTTATATATAAATTTGTAATTTTTTGTGTAAAAAAATATTGAAATTTTTTAAAAAAATCACATTTTTATATAACTTCATCGTGATAATAAATAGAATAGAAAAGGAATTATTAGAATGAAGAAACTAATTTTACTTGGTTCTCCACTAAGCATCGTATAGTGAACCCCAAATTCCGGACTTTTTGGAAAGTGATTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAAACAAATGAAATAGAGAAATAAAAGGTTATTTAAAATTAATGCTTGATCAAAAGATAAAAATTGTCGAATTATATCTTCGAGAATTTAGTATTTTAGAAATATCTAAAATAATGAATAACTCTTATTCAACATGCTATTCAGTGATAGAGAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATGAATTTAAAGTTATAAAAGTGAGACCACCATTTTAGTGAATTTCACTTTTATAACTTTCATATTAAAAATTAATTAAATTCTTTAATTATTTACTTTATAATTAATTTTAATATTAATAATAAGGAGAAAAACATGGCAAAATACACAGAACAAGAACAAATTCGTCGTGATAAACTAGAATTTTATAAAAAATTTGATGTAGTACCTTTTAAAAAAGCTTATGGTCTAGGTAAATTGAGTACAAGTGATGAACTTAATAAAAAATATGGTCAATTTGCTAGAGAAGAATTAGAAGCTAAAAAAGTTAAGAAAAATATTAGTGGTAGATTAATTACAGCTAGAGGACCTTTCTTAGTATTAAAAGATCGTAAAGGAACAATTCAAGTTTATTTCAATAAAAAAGAAAATCCTGAATTAACTAAAATAGTTGAAACATTTGACTTAGGGGACATTTTATGAGTTAGAGGTCTATTAATGAAAACTCATACAGGTGAAATGACAGTTAGGGCTCAAGATATTCAATTATTAACTAAAGCTCTTAAACCCCTTCCAGATAAATTTCATGGATTAACTGATACTGAAGAAAGATATCGTCACAGATACTTAGATTTAATTACTAATCCAGAATCAAGAAATACATTCATTATGAGAACAAAAATTGTTCAATGAATTAGAGATTACTTTAATAAACTTGATTATTTAGAAGCTGAGACTCCATTTTTGCATGATTATCTTTCAGGAGCAGCTGCTAAACCATTTACTACACATCATAATTCATTGAACCAAGATTTTGTTTTACGAATAGCAACAGAAATTCCGCTTAAAAAATTAGTTGTTGGTGGATTTGAAAGAGTTTATGAAATGGGTCGCATATTCAGAAATGAAGGATACGACACAACACATAACCCTGAATTTACAACTATTGAATTTTATGAAGCTTATTCAAATGTTGAAGGTATGATGAATAGAACTGAAACATTAATTAAAGAACTTTGCAAAAAAATTGGTAAAAGTAAATTTGTAACAAATGGTGTTGAAGTTGATTTATCAAAACCATTTAAGCGTGTAAATATGATTGATGAAGTAAGCAAGAAAACTGGTAAAAACTTTAGAAAAATTACTTTAGAAGAAGCTATTGAAGTAGCTAAAGCTTACAAAATCAAAATCGAAAAATTCTTTACTATTGGACACATTATTAATGCTTTATATGAAGAATTAGTTGAACCAACATTAATTCAACCTACATTCTTATATGGACATCCAATTGAAATTTCTCCTTTAAGTGCTAAGAGTGATGATCCAAGATTTACTGAACGTGCTGAATTATTTATTAACACAAAAGAATATGCCAACATGTATACAGAATTAAGTGATCCAATTGACCAATTAGAAAGATTCGAAAGTCAATTAGAAGAAAAGAATAAAGGTAATGATGAAGCAAGTGATATTGACTATGACTTTGTTGATGCTTTAGAATATGGCATGCCTCCAACAGGTGGTTGTGGAATTGGAATTGACCGTTTAGTTATGTTATTAACTGAAACAGATTCTATTAGAGATGTACTTTTATTTCCCACACTAAAAAGAATCAAAAAATAATAATATTATTTAATGCCCTAGATAAAAGGGTATTTTTTTATCTTTTTTCATAAAAAGCAAAAGTTAAAAATACATCTCTTTTACCAAAACAACTGTTTTATAGGCTTATTAATAAAATAATCAATGAATTATTGTGTGAAAAATTTATAAAAAATATGTATTTTCAAGTGATTTTTATCATTTAAAAAATACAAAAATAACATTATTAAGCATTTTTAAACATAATTTAAATATGCAATAAAAGATTAAAATTGACCAATGAATTGAATCATGTCAGGATAAATCTAATAAAAGGTTTTAAAGTTTTATAAGGATTAACAACTACTACAGCAACAGCTAAAGGCGCATTGTTAATTGCTTTAGTTGCTAGACATAATCAAAAGAAAGAGAAAAACAATAAAACCAGCTAAAGATACATATTTAGAAAATAAACAACAAATCGAAAAATGAACTAATAAATTAGTTAAATATAGCATTGTCAAAGATTCATATCCAAAATATGAGACTGAAATAGTTTCAATATAAAATCTTGCTAATGAATCTATTAAAAAAGTTGCTCAAGATAAAAAATATTTTAGCAAATGGCCCAAATAATCTTTTAAACAATATTAAAAGTTTTGAGTTAGCAAATGTTAGAGCAAAAGAAACTTATGACCAAATTAGACAAATGAGAAAGTCACATTTAGACAAATATTGCCTAACATTGTTACAATTGTTGAATATAGTAAAAAACATGATGTGGCAACTGAATTAAGACATGTTGTAGAGTTAATGAAAGTTGTAGACAATGATCTTTCAACTTATTTCAATAGTTTAACAAGAAATTAATTAAAAGGTAATTTTTGTCCTAACGGTTTATATAAATTAGTAATGGATGATATTGATAAATATAATAATGCAAAAAAGTAGAAACTTTAGGCTCAATAAGCTAAGATTTTTATTTTGCTTGTCAATTTTTTTATACATTTATTTTCTTATAAATGCTTTAAGTTTAAGTGTGTTTTGAATGAAAATATATTTTTTGGTTTTTAAATAAATTAGTTTTAAAAAATGAGAATTTTTCACATTTTACAATCTTTTTAATTTTGTTAATTTTTAGAGATTTAAAAATTTTAAAAATTTGAAAATAATTTTCAATTTATTTATGTATTTTTGCTTATATATATTATAAGGTATCAAGAAAAGTAAAAAAAGCTGTAAAGATTTTCATATAAAAATTTTGGAAAAAACTTTCAAAATACGCAAATTTTATTTATTTTAATTATTAAATTTATTATCTAAAATAAGTTTAGAACCAAAAATCAAAAAGGGTTCTATTGATTACAAAAAAGGAGATTTATGAAGAAAGAAAAATTTGTTAGGACTGTGCTAGGCGATGTGCCTGCTAGTTCTATCGGTATTACTGACTGTCACGACCACCTTATAAAGAACGGTGGCCCTGAAATGGAAGAACACATTGACTTTTTAATGATCGATGTTGAAGCTGCTAAAAAAGAACTTCAAGAATTCGTTGATCACGGTGGAAAAACAATGGTTACAATGGATCCACCTAATGTTGGACGTGATGTATTTAGAATGATGGAAATTGCTAATGCATTCAAAGGCAAAGCAAACATTATTATGTCTACAGGTTTCCACAAAGCTAAATTCTATGACAAATACTGTTCATGATTAGCTAATGTTCCAACAGACGACATTGTTAAAATGTGTGTCGCAGAAATTGAAGAAGGTATGGACGTTTACAACTATAATGGACCAGTTGTAAAACGTGGTAAAGCTAAAGCTGGTATTATTAAAGCTGGTACAGGTTATGCTGCTATTGACCGTCTTGAATTAAAAGCACTTGAAGTTGCTGCTAGAACCTCAATTACAACAGGTTGCCCAATTTTAGTACATACTCAATTAGGTACAATGGCTTTAGAAGTTGCACAACACTTAATTGACTTTGGTGCAAACCCTCGTAAAATTCAAATTTCTCACTTAAATAAAAACCCAGACCCATACTACTATGAAAAAGTTATTAAAGATACAGGCGTTACACTTTGTCTTGATGGACCAGACAGAGTTAAATACTACCCTGACTCAACTCTTGCAGATAACATTAAATACTTAGTAGACCAAGGTCTTCAAAAACATATAACATTAAGCCTTGATGCTGGTAGAATTCTATACCAACGTAACTATGGTTTAACAAAAGGTAAAGAAACATTTGGATTCTCATACTTATTTGATAGATTTATTCCTTTACTTAAAAAAGTTGGTGTTCCTCAAGATGCTATTGATGACATTTTGATCAACAACCCACGTGAAATTCTTGCATTTGATGAACCTCGTAAATATGAACCATCAAAAGTATCAAACGAATTAAAACAACTTAAAAAAAATCTAAAACTAGATTAATTTAAACTTTTATATATTTATTAAAATTAAACTTAACTTAATTAATTATTCATTTATTTTTATCCATAAAAACTTAAAAACATAAAATTACTTATAACTTATATACTTATTTAACTCAAAATAATTCCACTTTTCGGGGGAAGGATATCTTATGTCAGAAAACAAGACAAAAAGATCTTTTAACTGAAAAGCTTTAGTTGGTGCTCTTGTAGTTATCGCTATTTTTGCAGGATTCTATGTTCTTGCTTTAGGTCTCAAAAAATGAGATTTTAAAGCTGGTACATTATTCTTTGCAAAAGACTTATTATTAAATAACTTCTTAGGAATTAACGCTGTATTAATCGGATTAATTGTCTTCATTGGCTATTTAATACTAGGTAGAGGCTTTTCAGACTCACTTATTGGAATGCTTAAAGCCATGATTGGTGTCTTAATGATGCAAATCGGTTCAGGAATGATTGTTGGTTTAGCTAAACCAGTGTTCCTTGCATTCTCTAAATTCGGTAAAGGGCTTACACCACTTGATACATACTTAGGACAAGTTTCAGTAGAAAACTTCCTTAACTCAGTTGGTCAAGGTTTTGCTTCATGATTAGCATATGCCCTATTAATTGGTTTAACAATTAACATTATTTTAATTGCATTAAGAAGATGAACAAATGTTCACTCACTTATGATTACAGGACACGTTATGTTCCAACAAGCAGCTGTTGTTGTTCCAGTTGTACTTGTATTAATGTTCTCAGGACAACAATTTAGAACAGCAGAAGGTGCTATTAATGTAGGCGGCCAAGTCGGTACAATCTTCTTCTCAGGTTGATTACTTGGTACTTACTGAGGTGTAGCTTCATCTTCAACAATTAAAGGTTCAGATGCTGTTACACAAAAAGCTGGTTTCTGTGTTGGTCACCAACAAATGTTCGGTATTGCTATTGCATACCAAATTGGTAAATTCTTCGGTAAAAAAGAAGATTCAGCAGAAACTAAAAAACTTTCAAACAAAGTTAAATTATTAGAAGACAATATCTTCACACAAAGTGTATTGATCTTATTAATTTTCTTAATCCTTATCTTAATTATTCAATTCGCACCTGCAGGTGCATCAGTAAGATTTGGTTTAAGCAATGGACTTGTAAACAAAGCAATTTATGGTTCATGAACACCAGGCGGTGGAGCAGTTTATTGACCAATTAACCTTGTTTTAGGGTCATTACAATTAATTGCTGCTATCCTTACATTGCAAGCTGGTATTCGTATGTTCGTTTCAGAACTTCAACAAGCTTTTCAAGGAATTAGTGAAAAATTAGTTCCAGGATCAGTTGTTGCTGTTGACGTTGCTGCCACATATGGTTTCAGCCAAAACTCAGTTACCTATGGTTTCTTATTTGGTTCAATTGGCCAATTCTTAGCTATGGGTCTTGTAATTGGATTAGCTCAAATTCCAAACTCAAGATTTGAAATTGTTATGGTAGTTCCATTATTCATTACATTGTTCTTTAACTCAGGTTCAATTGGAGTATTTGCAAATGCTTCAGGTGGATATAAAGCTACTATTGCAGTACCAATGATCTTTGGTTTCGTTGAAATTATTGTTGTTGCTCTTGCTTTAAGTATGATTAAAAACTTTGGTGTAATGCACCAAGTAGATCTTGACTTCCAATACTTTGAAAAAACTCTCACAAAAGCAGATGCATTCAAATCATTTACAGATTCATTAAATGCAAATTCTGCTAACTTGTTTATTCAAGGTGATGCTACTAAAGCTGTTTCAGCAGCACAATTAAATGATGCTATTACAAACATTAACAGTCAAGATTCATTTAAAATCTTAAAAAGCTTAAGTGAAGTTACAACAAACTGAACTTTAGGACATGGTAAAAATGCTAAAGCTATATCTCTCCCAGATATGCAATACTTATCAAGATATGTTTCAGACCAAATAGCTAAAGCTAAAGATATGAAAGAAATCTTGAAATTATCTTCATTCCAAAGAACACCATTCTTAACAGGATTTAATGGTATGTTTGACTGAACATGTATCTTTGGTTCAATTATGATTCTTGGTGGATGACACCCATATGCAGCTTACATCGTATTCCCTCTTTACGTTGTTGGTATGATCTTTGCTGCTCAATTCTTTGACTCACACAGACAACACAAACCTACAGTATTCCAAAGAATGCTTAAAGTTAAAGTCGAAACTTTAGAAGATCAAAAGAAAGCTGAAGAAGTTGCTAATGCACCAGTTGAAGAAGTTCCTCAACCAGAAGTTGCAGAACAAGCTGCTTAATTAGGCAAAAAAGTAAAAATAAAGGCAGAATTTTCTGCTTTTATTGCTTATATTAATATAAATTATATATACTAATTATTAAAATTTAGGATATTAACATGAAAGTATTATGTGTTTGTGGCTCAGGTATGGGCACAAGTATGATTATTAAAATGAAAGTTCAACAAGCTATGAAAGAACTTTCAATTCCAGGAACAGTTGAAGCTTTAGGTTTAGGTCAAGGCAAAACAGTTGTAAACAACTTTGATGTTGTTTTGTGTACCTCAAACTTTGTTTCAGAAGTATCAAAAGGCAAAGCAAAAGCTTATGGTTTAAAAAACATCATGGATGTTAATGAAATCAAAGCTGCTCTAAAAGATGCATCAAGCAAGATGTAATAATGGCTGATAAATTAAATTTATTAGATAACTTAATTGTTAATGATTCAATCGTACCTCATGTTGATGTTAAAGATTGAAAAGACGCAATTAAGAAAGCTTGCTTACCATTAGAAAAAGCAAAAGTTATTAATTCTAAGTATTATGACTGCATTTTAGAGTCAATTAAAAAACATGGACCATATTTCATTTTAGTAGATGGTCTTGCTATGCCACATGCTTCAGCAACAAAAGACGCTGTTTTTGGAAACGGATTCTCACTTATTACTCTTAAAAAACCTATCAAATTTGAAGGAGATGAAAGAGAAGTAAAAATCCTTATGGGACTAGCCGCAAAAGATGGTGAAACTCATACATCTGTTGCTATTCCACAAATTATTGCTGTTTTTGAAGATCCACAAAATATTGAAAAAATAGCACAAGCTAAAACAAAAGAAGAAATTATAAATATCATAAAAGGTGTTGATTATACTAAATACCTTAGTTAGGACTTAATATGAAACCAATGTTACAAATTGCTTTAGATAACTTAACAATTGAAGATGCAATTGCAAGTGCCAAAAAGGTTGAAAAATACATAGACATTATTGAAGTAGGAACTATTCTTATTGCTTCAGAAGGTAAAAAAGCTATTAAAGCTTTGAGAGAAGCATTTCCTACAAAAATCATTGTTGCTGATGGCAAAATTGCAGATGCTGGGAAAGTTTTTGGAAAAATGTTTTTCGAAAATGGTGCTGACTTTACAACATGCATTTGTGCAGCTGAAGTGCCTACAATTGTTGAAACAATGAATATAGCCAAAGAATATGGCAAAAATAAAGAAGTTCAAATTGAATTAACTTCAAACTTTACTTGAGATCAAGTTGATGATTGAGCAAAAGTAAAAGTTCCTCAAGTTGTTTGACACCGTTCAAGAGACAGTCAAGCTAGTGGAGTTAAATGAGGACAAAAAGACATAGATGCTGTTGCAAAACTTGCTAAAAAAGGTTTTAAAGTAACTATTACAGGTGGTGTTGCTCTTGAAGATATTAAACTATTTAAAGATATTCCTATTTACATCTTTATAGCTGGAAGATCATTAAGAGATGCTGAAAATCCTGAATTAGCTGCTAAAGCCTTTAAGGACGAATTTGCCAAATATTGAAAATAAAAAAAGACTTCTAGGCATCTATGAAAAAGCTATAAACAACAAATTTAGCTTAGAAGAAAAAATTATAATTGCTAAAGAAGCTGGTTATGACTTTATGGAATTTTCAGTTGATGAAAGTCCTCAAAGATTAGCTCGCTTAGACTGAAGTGATGAAGAAATTACTCATGCTCAAAGCTTACTAATCAAACACAAATTTAACTTTAACTCAATGACCCTTAGTGGTCATCGTAAATATCCTTTTGGTTCTAAAGATCCTAAAATCAGAGCTAGAGCCAAAGAAATAATGACTAAAGCAATTATTTTAGCCAAAAAATTAGGTATTAGAACTGTTCAACTTGCTGGTTATGATGTTTATTATGAACCAGGTGATGAAGAAACAAGAAAAAACTTTATTGAAGGAATGAAATGAGCAGTTACTGAAGCAAGTAAATATTCAGTTATGTTAGCTTTTGAAATTATGGATACTCAATTCATGGGAACTAATTCAAGAGCAATGACTTATATCAACATGATTAATAGTCCATATTTACAAATCTATCCTGATTTAGGTAATATTTATCAATGAAGTAAAAAAGAAGATTTGTATAACGAATTTGAACTTGCTAGAAATCATATAGTAGCTTTCCACTTTAAAGATACTGTACCTGGTAAATTTAGAGATACACCTTTTGGAACTGGCACTGTAGATTTCCCTTATCTTTTAAAAATATTAAAAGATTTAAAAATGAACCAACCTATTATGATAGAAATGTGATCTTTAAATAAAGAAAATGAAACAAAAACACAAGCTATAAAATACATTAAAGATGCTCATGATTTTTATGACAAAATGTGAGCTAAGGTAGGAGGTGACAAATAATGGCAATTGATAAAAAATATGAATCTGAAATCAAAAAACTTAAACAAGAAGTTTATGAAGCTAATATGTTATTAGTTAAATATGGACTTGTTATTCATACTTGAGGTAATGTATCAGGTATAACACAAGACCGTAAATATATGGTTATTAAACCAAGTGGTGTTTCTTATGACACACTTAAAGCTGATGACATGGTCATAACTGATTTAGATAACAATGTATTTGATTCTAAATATCGCCCTTCTTCAGACACACCTACTCATACCTTACTTTATAAAGAAAATCCTGAAATAAAAGGTATTGTTCATACTCACAGTCCTCATGCAGTAGCTTTTGCACAAGCAGGTAAAGATATTCCTTGCTTTGGTACAACACATGCAGATAATTTTTATGGACCAGTGCCATGTGCTAGAGCTCTAACTCCAAAAGAAATTGATGGAGAATATGAACACAATACAGGACTTGTAATAATAGAAACATTTAAAAAACGCAAGATTGACTGAAAAGCTTGTTCCGCAACATTAGTTAAAGAACATGGGCCTTTTGCTTGATCATTCAAATCTCCTAAAGATGCTGTTGACTTAGCCTTAACACTTGAACAAGTGGCAAAAATGGCTATTAATACCTTAATAGTTTCAAATAATAGTGCTCCAACTGCGCAAGAAGCATTGATTGAAAAGCACTATAACCGTAAGCACGGAAAGAATGCTTATTATGGTCAAAATAAATAATATAGACTACTTCATTTTTGATTTAGATGGTACACTCTATGATGATAATAAAAACATCTTAGAATCAACCAAAAACACTTTAAAACAGCTTCAAAAGGAAAATAAAAATATAATAATTGCTACTGGTAGACCTTATTATATGAACTATAAATTAATTAAGGATTTAGATATTAAACTACCAATAATAGCAGTTAATGGGGCTATGATTTATGATCCTATCACTAAAGAAGTGCCTTATTTTCAAGGACTAGACAAAAAAGTTGTTCAAGAATTAATTAACTTTTTAGAAGAGAATAGAATTGATTTTTTAGCTTACGATATTAATGGTATGAAAGGTAATAATTTCTCGAACCCTTATTGATTCGAATTTATGATCTATAAGAATGTAGTGGAAACTAATCCTTATCGTTGAGATTATCAAGAAGGAAGCTTAAAAGAAACTTGTAACCAATTTAACTTTGCTAAAATATTAATCATCACTCAAAACGTTCCACAAGAGAAGATAGACTCCTTACTTACAATTGCAAGCAAATTTCCAAAACAAATATATACGGTATCTTCTCAAAAAGGTGTTATTGACATAATGCCAATAGGAATATCAAAAGGTGAAACATTAAAAAAAATAGCTAAGATTAAAAATATTGATTTATCAAAAGCAATGGCTTTTGGTGATGCTGATAATGACATAAGTATGTTAAAATCTGTTAAAATTTCAGTTGCAATGGGTAATGCAACAGAGAATTTAAAGAAAGTTGCAACTTATGTAACAGATACAAATAATAATGATGGAATTGCCAAAATTATTAAAGAATTAGAGTTAGTAAATGACTAAAATTAAAGTTTATAAAGTTAACATAATCGAAAATAGATACTGATATTGTCCTTCACTTTTTACATTCAGTAGAAGGTTATGAGCTTCAAGACCATTTGCTACTCTTGAAGAATTAATGAGACATTTAGAAATTAAATATAATCCAGGGTATTTTAATTTCAATGGTGATCTAAGATTTAAAGCATTCAATGAGTTACAACGAGTACATAAAGTAGGTATCTCAATTAATCCTTCAGCGGTTAAAGATAAAGACAATTATTTCAAGTTTCATATTAGTGAAAATGTTGAAGTAGTGTTAGATGATTTATCACTAAAATTAATTAGTAAAGGTAGATCGTTTGCCTGTCCAATGCATTTCTTTGATGATTTATACTTAGAATATTTTGATGAGAAAAAAGTAACTAAAAATCAAAAATTGAGATTAACTTGAAGAAAGTATTTCTTCAATATTGAAGTTGTTGAAAAAGAACAAACAGAATAATAATAAAAAAATGAACTTTTGTAGAGTGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTTAAATTTAAAAAAGTAAAAAGAAACAAATGAAATAGAGATATAAAAGGTTATTTAAAATTAAAACTTGATCAAAAGATAAAAATTATCGAATTATATTTTCAAGAATTTAGTATTTTAGAAATATCTAAAATAATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTTTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAACAAACAACTCAAAGCATGTCAAGAAAGGGAAATTGTTTAGACAATAGTCCTACTGAATGTTTATTTAGTGTTATAAAAAGAGAATTTTGATTTGGAGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGGAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGTCCTGTCCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATTTTTGGGGTTCATACCATTGCTTAAAAACAAGGGTTCATTTTTTGATATTATTTTGTTCTAATTAGCCTATAAAAAAATGTAATAAGTAAAAATTGCATTCAATTTTTGTTTAAATATTAAAAACAAAAATTGAATTATTATCGCTTGCTTGAGGGTCTATTTGAGTGTCTTTTTTGACCTTAACTTCTTTTAGGTTTGTACATCCTTTAAATGTATTTGCTTGTATTTGATTCACGTTGGTATTTGTTAAGTCTAATGTTTCTAATGATTCGCACTGTTTAAAGGCATTAGTTTTAATTATTGTTGTGTTATTTGGGATATGAACGGTTTTTAAATTAAAACACATTTCAAAAGCACCTTCAGTGTTTAAATTTACAGTATCTGGAATGTTTATTTCTTCTATTGAATTACAATGGCTAAAAGTTTCTTTTCCAATTATTTGAAGTTGACTGTTGAAGATAATTTTTGTTAGACTTCTACAACCATAAAAAACACCATCACCTAATTTAATCAAATTTGCGGGGAATGTAATTTCAGTTAATGAAACACAATAGGCAAAAACTTTGTTTCCAATAGTTTGTAAAGATTGAGGTAAATGAATTTTAGTTATTTTATCAAAACCATTAAATACTTCATTTTTAATTTTAGTTATACCTTCTGGTAAAGTAATTTCACCCGCGTAGTTTTTATTTTTGTTAAAAGATATTAGTTCATTGTTAACTATTTCAAAATCTTTGGAGTTGTTGTTTGAAGTTTGATTATTACAACTTAATGTGCTTAAAGGTAATAATGGTAAACTTCAAATTAAGTTTGATAAAAGTATTTTCTTTTTCATGTTAGTCCTCCTTTTATCTATATTTTTATTCTACTCTTTTTTGAAATAATAAAAAATGAACTCGCAAATTCGAGTCCACTTTGTAAGTTAAATTATTTAACAAGCATTAATTTGTCAGCACGTTTAGTCATTTTTTCGCCAAGAGCTGTATCAATAATGTGTTTGTGTTCTGATTCATTAAGAATTACAACGATAACATCACTTACTTTATTTTGGTTTGATAAGTATTCAACATCTACTGTACATAACAAGTCACCAGCTTTAACTTTTTTGCCTTGTTTTAATTTGGTTTCGAAACCTTCGCCATTTAAAGCAACAGTATCAACACCAATGTGAATAAGTACTTTAATACCTTCTTCTGTTGTAATACCATAAGCATGTTTTGTAGGGAATACTGTTTCAAGAGTACCTGAAACTGGAGCTCAAATTGAAGCTTTCTTTTCAGCTTTAAGACGAATTGCAATACCATCACCTAAGTATTTATTTGCAAATGTTTCGTCAGGTACTTGACTAAGAGGAATTACATCTCCAACTGCTGGAGCAAAGAATTCAACTGGACGTTCTAAGTCGTTAGATTCGTCAACTCATTTAGTTTCTGTCATTGATGATGTTTCAACTTGAGGTTCAGGTGTAACTGCTGGGGTTTCTTGAACAGTAGGTTGTTCTTGTGTTGGAGCTTCAACTACTACAACTGGTTGTGTTGGTTCGCTTTTTGCTTCAACAACAATGTCATCTAATTCACCTGAAGCTAAAATTTTTCTAATGTTAGCATTCATTTGTTCAGCAACAGGGCCAACAATAACTTGAACGTGTTTATCACCTTCTCATTTAAGTGCAACTGCACCTGCTGCCTTACATTCAACTTCATTTACTAAGTTTCTATCGTTAATATCATATCTTAATCTTGAAGCACAGTTATTTGTGTTAACGATGTTTTTTGGACCGCCTAAAGCAAGAATCATTGCATATTCTCTTGCGTTTCTTGCTGAAGTATCTTTACTTGAAGAATTTTCATCATTTCCATCTTGTTTAGCTTGGAAGTCTTTTTTGGTGAATAATCTAACTGTTTCACCACGACCTGGGGTTGCCAAGTTGAATTTCTTGATGAAGAAGTAAAAACCACCAAGATAAATAAAGAAGTAAGGAACTCCGACTGCTAATGCTCATCAGAAGTTTGTACCTTTCATCACAGGAATAATGCCATAGATGATTAAGTCCATAATTCCACCTGAGAATGACATACCAATGTGAACGCCAAGAACGTTAGCTAACAAGAATGATAATGCACAGAAGAATGCATGGAATCCCCAAAACAATCATGGTGCTAAGAATAAGAATGTAAATTCAATAGGTTCTGTAACACCAGTAATAACTGAAGTAAGAGCTGCAGGAATAACTGTACCAGCTGCCACTTTTCTATTTTCTTTAGGAGCCGCTAAAATCATAGCAACAGCTGCACCCGGTAATCCTAGAATCATGAATGAGTATTTGCCTTGCATGAATCTACCTAATTTAATGCCTAATTCACTAGCAACAAATTTATATAAAGGTAATGTATGAACTTCATGTGCTGGTGTAGCATCTTTTCATGTTATAGTGTTGAAATTTAATTTCATTACACCAATAGCAGCATATGAGTCACCCATTCATTCATTGTGTTTAGAAGCCATCAAAGCTTTTCAACCATTGTACGCAGTTAATGCAGTAGCATCTCCTGAAAAAGTATTTCCTCTTGCTTCTCAATCTTCAAGCGCTTTAAGAATATCACCGCCTGCATTTGTGTATCAAAGAGGTGCATAAAATGCGTGGTGTAAACCAAATGGAATTAATGATCTTTCAATATAACCAAAAACAAATGATTCAATTCCATAAGGTACATGGCCAAGACTATTACCAAAGTAACTAAGACCAATACCAACTCATGGTCATATAAGGAGGAATAAGAATGCTAATGGAATCATAGCAACTATTGTTATTAATGAAACAAAACGTTTTCCTCCAAAGAATGAAATAACAGTTGGAAGTTTAATTGTGTGTAATTTGTTGTATAAGTATGAAACTATTAAACCAACAAAAATACCACCGAAAACTGAAGTTTGAAGAGTTTTGAATCCTAATGAAGAACCAACGACTTGTTTAACTTCAGCACCAAATTTACCTGTCATTCAAAGAATGTTATATCCTTTTAATGTTTCATAGTAAAGTTGTCCTGTTTTATCATCTTTAAGTATTGTTTTACCTATTAATTCACTAGCTTGTTTAGTGCCATCAATATTAGTTTTTTCTATAACACTTTGAATAAATGGAGCTTGTATTGCTGAGAATACTAAGTATCCAACTATTGCGGCGAAGACACCGACTCCGGCTTCGTCGGTAAATGCCACAACAAAGGCAGCGGCAAACAATAGCGGCATTGCTCCAAATATTGGGTCACCTAATAACTTAATAAAGTCACCAATTACAATACCAGCTTTAGCAGCAGATGATAATGGATTGTTGTTAAGAACAATCGCTGATTGACTTGATATTGTTGCACCAACCCCTAGAAATAGACCAGCTATAGACATAACGGAAATTGGTAACATGAAAGCACCAGAGATTTTACTTAGAATCTTACGAAAATTTCCCTTACGGCTATTGTCTTTTGGTTTAGATTTTTTGTTTTCTATACCTGTAGTTTGTGCTGTAGTCATTTTTCTCCTTTCATATACATTTAATAAAATTACCCTAAAATCTAATATTAATAATAAATATTAAGGTTCTTAAATTATAAAAAGAAAATCAATAAAAATAATTTTCAAAATTAACTATGTGAAAAAAATAAGGAATTTATTCCATACTAATTTTTTAATCATAATCTTTTTAAAAAATTGAAAAACTTTAAAAAAATATTTTACTAAAAAGCCTATAAAATAGATAAATTTATCAATTATTAAAAAAGAGGTTAATGTAATTGAAATTTGATTATAAAATGAAAAAATTTTTAGAGAGGTAAAAAAAACTTTCACATAAAGCAAAAATTTTAAATAAAAAAAGGACTTTTTTGAGCCCTTTTTTTACATTGAAGATTAATATAATATTTTGAAATAAAATGAGAAAAGCATCCGCGCACAGCACTTTTCAATTATCTAAAGAAAGGAGTCTATATACTCTTAAGCAACTATCCCTATGGGTATAGTAATTGTCCATCTAAAGGGGACACATCGACATCATTATATACCATTATTGGGATTAAGTCAAGAAAAAAATATACAAAATGGTTGGTACAATTTTCTTTAAAATTTTGAAGATATTAATTTATGCTTTTACTTTACAAAAATTCTTTTTTGTATAAAATAAAGTTAAATTTATATATTCATATAGTAAAGGGGTTAATATGAAAATTTTTAGAATTAAGGAAACTTCACCTTATGTTACTTTAGCTTTAGAAAATATTATTATGCATGATCATGATTTAAAAGGTGATGTACTTTTAATTTATCAACATGATAATGCTATTATTATTGGTAACAATCAAAATGCATATGAAGAAATTAACCGTTCTTTTGTAAAAGAAAATAATATTAAATTAGCTCGTAGAATGTCTGGCGGTGGAGCTGTGTATCATGATTTAGGCAATATTAATTTTAGTTTCATAACTGACTATGATAAAAAAGGCGGTTATGAAAGATTTCTAACTCCAATTATTGCTTTTCTTAAAAGCTTAGGTCTAGATGCTCAATTTAAAGGTCGCAATGATGTTGTGGTTAATGGCTGCAAAATAAGTGGTAACGCCCAATATATTAGTAATAATCGAATAGTAAGCCATGGAACATTATTATTTGATGTTGATTTAACAAAATTATCGAAAGCACTTAATCCTGCAAGAATTAAGTATGAATCTAAAGGCATTCAATCAGTTAGATCTAGAGTAGTTAATATTTATGATGAATTATTAAACAAAATGACAGCTGAAGAATTTATTAGTCGTCTAATTAATTATTTTGTTAAGAATTTTAAAGGAGAATTTATTGATATTGATTATGCAAAATATCAAGAACAATTAAACTCTTTACAACAAAAATTTGCTTCTGAAGAATGAATATATAATAAAGCAGCAACTTTTAAATATAAAAATGGAGCTAAATTTCCTGGTGGAATTTTAGTAGTTAAAGGTAATATTGAAAAAGGTACTATTAAACAATTAGTTTTTGAAGGTGACTTTTTAAGCAAAAAAGATATTCATGAAATTGAACCCTTATTTAGTAATGTAGCTTTAAATGAACAAAGCATTCTAGAAGTTTTAAATCAAATTAAAAACTTGAATGAATATTTTGGAACTGTTACTAAGGAAGAAATTGTTAGCTTGTTAATTGGTTAGGAGGCTAAAATGTATAAAAGACAATCAATTGAACTTTTAGGCGAAACAATTTATTATGAAAGAATAGATTGTTTTCAAAAAGAAAAAAGACCAGCAACTGTTTTACTTGTGCACGGTTTTAATTCTGATTCAAATACCTTTACTAGATTTATTGATTTTGCTGAAGTAGTGGCCGAAAATACACCCGATAATGACGAATTAAGAGAAGAATATAGAAACTTTCATCGTTTCAATTTATATGCTTTAGATTTGCCTGGTTGTGGAAAATCTGGAAAAAATAGTCAAATAACACTTGATTTATATAACAAAATAGTAGTTGAATTTATTAACACAGTTTTAAAAGACCACGAAGTAATTTTAATTTCGCATTCAATGGGTTCAGTACCTTGTTTGTATGCTAAAAAAGAATGCAAAAACGTAACTAGGTTATTTATGGTAGCTCCTTTAAATTATGCTTTAGTTGACGATAAGCAAAAACTAATTAATCTTAAAAAATGATTGATTCCTGCTAATGTTGAAGATGCTAGAAATTCATTAAAAGCATTGGTTTATAATCCTGAAGATTATTATTTAAGAAACTTGGATTATAATGCTGAAAAATTAGCGAGCAAATATAATGAAAACAAAGAAACTTTTTCTTATCTATTAAATGAACAAATTTTAAATAAAGATTATTTATTAAAACATGTAAAGCCTTTATTTGCAAATCCAAATGGTTGTGTAATTATTTATGGTGAAAATGATGAATTTGTGACACCAAAAGAAATTAAAAAAATAGAAGAAGATTATGGTTATAAAATTGTGCAATTAAATGAATGTGGACATGCTGCATTTTATCAAAAATTAAATACCATTTATTGAATAGTTCAAAAAGAATTAAATTATAAATAAAAATAGAACTTTTTTGCAAACTGTTAATTCCAAAAGTGAGACCCCATGTTTTAGAGATCTCACTTTTATAATTTACATTATTTATATTCATTGATAATTATTGAATGTTTAGTAAAATAAGTAATATATATTATATTAAAAGGAAAAATATGAATAGGCAAAAAACTTTATATCAACCTAAAATTTCAATTTTAATTCCCTTGCATAATAGTGCTAAATATTTTAATTCCACTATTAAAACTATTCTTAATCAAAGTTATGAAAATTTAGAAATTTTATTCCATGATGATGCCTCTAATGATGAAACAAGAAAAATCTTAGAAGATTTGACCATTAAAGATTCTAGAGTTAAATATATCTTTTCTGATACTAATATTGGTTCAGGATATTCAAGAAACAAATTAATTGAAGCTTCTACCGGAGATTTCATTTTTTTCATCGATGATGATGATGAATTTTTAAGTCCTAAAATAATAGAACGTTGTGTTAAATATTTAAAGCCTAATTTAGAAATACTTTCAACACAATTTAAATATAAATTTGATGATTTAGGTATTAAAATACCTATCAATAATATTGTTAAAACATTGAAAATCAATCCTTCACCAATGCATTATTATTTGAATACCACAACTTTTGCTTGAGGACATTTCTTCAACAAAAAATTTTTATATGATATTGATGTTAATTTTAGCAAAGTTAAAAAATATGAAGATATTGCAGAAATGGGAAACATTTTTTCCAAATGTAAGAATTTTAAAAGAACTTTGATTTATTCTATAATTTATCATAGAAAAACCAATTCATTAACTTCATTTGATAAAAACTATATCAATAAAATTGAATGTATCAACCAAGCCTACAAATTTAATTTAGAAAACATAGTATCAAATTTAAAAAATGATGTAAGTTTTAAAATTCTTAAAAAAACTGTAAATTCCTTATTTATGGAACATTTAAGTTTGATTAGTGTTTACTATATCAATTTAAAAAATCGAAATTTAAAAAAAGAATTTAAAAAATTCATCAAAGATATTTATCCTTCAATTTTAGAAATATATAATGGTTTTGATGTAAAAATCAAATATCGAAGAATTTCTGATTTACCAGTTTTTGTTACTAAATTTTTATATAAAATACCAAAAGTTAAAAAATAGAAAGGAGAAATAATGACTTATTTATTAATTGGTGGAGCAGGTTATATCGGAAGCCATGTAGCTGAAGTTATAAATAAAGAAAATCAAAATAAAGTAATTATTTATGATAATCTTTCAACAGGATTTAAAGAATTTATTGAAGAAAAAAGTAAATTCATTCATGGAGATATTTTAGATATAAAAACATTAGATTTAACTTTTAAAAAAAATAAAATAGATGTAGTAATTTATTTAGCAGGTTTAATTAAAGTTGGTGAAAGTGTACAAAAACCACTTGACTATTATGAAACCAATATTCAAGGCTTAGTAAATGTTTTAAAAACAATGAAGAAGCATAATGTTAATAATTTGGTATTTTCTTCATCAGCAGCAGTATATGGTAATAACTCATTACACGATGGTTATTTTTTTGAAGAAGACCCAAAAGAACCATGTAGTCCTTATGGTAGAACCAAATATTTTGGAGAACAAATTATCAAAGACTTTGCTAATACAAATAGTGATTTCAAATATACTTTTTTAAGATACTTCAATGTTGCCGGTGCTTCAAAAAGTAAAAGAATTGGTTATTTAACCAAAAATGGTGTTGCACCGACTCATCTTATACCTGCTATTAGTTATTATGCTTTTGGTTTAATTCCCGAATTCAAAATTTTTGGTAATGACTATAACACACCTGATGGGACATGCATAAGAGATTATGTATATGTATATGAATTAGCAGAATTACATTTATTAACTGCAAAAAAAATGATTAAAGAAAAAACTTCATTATATTACAACATTGGTAGCTCAAAAGGCTTTTCAAATCTTGAAGTTGTAAATGCTTTTGAAAAAAATTTAAGCAAGAAATTAAATATTGAATATGGTCTAAGAAGAGCAGGAGACCCTGATAAATTAATAGCTTCAAATAAAAAAATATGTAAAGAATTAAATTATGAAGTAAAAACTAATTTAGATGAAATAATTTCTAGTGAGATTGAATTTAGAAAAGAACATTTATAAAATGTGTGAAAGTTATAAAAGCGAGACCCACCATTTTAGTGAATCTCACTTTTTTTGTTTTGTCCTTGTATAATATAAATTGCATCAAAAGAATTAAAAATAAAAATAATGAATTTATAACTGAATATATACATGTTTTTGTCGAAAAATACGATCTTTTAACATTTATGAATTCGTTAAATAGAAATAAATTACATCCCAATAATGTAAGAATTGAAACAGTTATTAAAGCACTAGAAATGTTTAAAGAATTAAATAACTTTAAGAATATTCATAAATCTTTAAATTTAAAACGCGAAACATTTCAAAAACTATTTGATGACTTATCAGATGATAGAAAAATGATTTCCAAAACCGATAAATTTTTTAAAGGTTTGACGTGCCCACATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTTCAGTTTTAAATAAATACAGAAATTGTAGAAGAATAATAGTTAATCAAAAAATTAAAAATAAACACAAAGATTGAATTAAATATATAAATCAAAATTCAAATGTATATGATAGATTATGCAATGAAAATAGAAAAAATTCTTTAAGAACTTTTTGCCAAAAATCAGTTCGAACATTAATTGATGAAGCTAAAGAAAAATGCTTAAAAATTAAGTTTCCACACTTCACTACAATATACAAAAATATAAACATTTTAATATTTGATATAAATATCTCAATTTTGCCCAATAAGTCTGTAGGAAAGGGAGAAAGGCATAAAAAAGACCCTTCAATTCCAAAGAAAAGAAGAGTCGGATTACATATAAGTTTAAGACCAGAAATTACAACAATGTTCGATTTTGAATTTGATACAGTACATGGCAAAAGCGATGATTTATATCATATTGCTGTTTTAATGAATAGAGCAACACGAAATTTTATATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAATACATATGATTGATGAACATGAATTAAATATTAATACTTTAACCATTGACAATGGTTCAGAAAATTACACTTTAGATCAGATCAACACTATTAAGCAAATATATCATTGCGATGCTTTCAATTCAAGTCAAAAAGGTAGAATTAAAAATTGTAATAAAATGATAAGAAAATATAGACCAAAATCAATTACTTTTGATAATTTAACACATGAATGAATTGAAATAATTAACGAAAAAATAAATAATTTTGTAAGACATGAAGTAGATGATTTTCCATTTGCTATGTCAGCAAATCAATTTGAAAAATTTATTCAAGAAAACAAACATTTATACCCATCATTTTATACAAATTTATACGCTTAATTAAATATTTTAAAAAATAAAACTACCTAACTCAGCTGAGTTATTTTTCCTATTGCTTTATTTCGAATTATTTTTATTTATTAACAAAAATAGCAAAAAAACTTTTATAATATCTTTAGCAATTTGTGTTTAACGGACAAATTATTTTAAAAATCCATAATGGTATGTCTCACTTTTATAAAAATTTTAATAAGATTAAATTAATATTTTACTTCTTAATTTTTGATATAATTTGAAAATTATCTTTTGAATTATTTATATTATATTTTAAATATAATTAAAGAGAAAAATAATAAAATACAATTTACATTTATATACTTAAAACAATATTTTGAAATATTATTTTAGAAATTTGTAGTAAATTTAACAATTAAAAAATTGGATTTTATGTAGTAAGGAGTGGTAATGGTATATCAAAATGTAGTTTATATAGTTATGCCAATTATTGTAGCGATTTTTTTAATTTTTTCATTTCAATATTGATTTGTTTTATTTTTTGGTTTATTTGCTAAGCCAGTGAAATTTAAGACTCAAACTCAAAAGCTAAAATATGGTATAGTCATTTGTGCTAGAAATGAAGAAAAAGTTATTGCTGAGTTAGTAAAAAGCATAAAAAATTCTCAATATGATCAAGATAAACTCCAAACATTTGTGATAGCACATAATTGTACAGATAAAACAGCGGAAAAAGCAAGAAATGCTGGTGCTATTGTTTATGAATATTCTAATTCTAGCGAAAGAACTAAGGGTTATGCATTAAAAAAAATATTTGAATTCATAGAACAAGACTATGGTATACAAAGTTTTGATGGATATCATATATTTGATGCGGACAATATAGTTGATAGTCTATATTTTGAAAAAATGAATGATGCTTTTTTACACTATGATAAAAAAAATGTAATTACTTCATTTAGAAATACTAAAAATTTTGGTAAAAGCATTCAAACAATTAATTATGGACTTATGTTTATGTTCCATACAACCATTGAATCAAACGCTAGAATGAAGTTTAAGATGTCTTCAAAAATTTTGGGTTCTGGTTTTTTAATTAGCAATGAAATGGTTAAAGATGGTTGAAATATAACTATTCCATCCGATGATTCAGACTTTACGGTTGAACAAATTTCAGAAGGAAAAAATGTTAAATATTGTGATGAAGCAATGTTTTATGATGAACAACCTACTAAATTTAAAGATATGTGACGTCAAAGATTGAGATGAGTTTTTGGAAATAGAATTGTTTGAAGAAAAAGATTTAAATCTTTGATAAAAAATATATTTTCATCTAAAAAAGAGGATGATAAAAAAATATCAAAGTCTTCTTCTATTGATATTTTATTTATGATATCACCAATTAGTTTTATAGGTTTTTTAATTTTACTTTTAAATATTTTGTTAGTTTCACTTGGACCTATTTTTGGAGCTAATGCTACTCAAATGTGAATTGATTGAGCAATATCTTTCTCTATAAGTCATGCAATAATATACTTAATATTATTTGTAATTGGTATAATTACATACGCAAGAGGTAGAAAAAGAGTTCAGCGTGTTCCATGGTGAAAAATGATAATATCATTATTTATTTGACCTTTGTTTTTAGCGTTATACTTTCCTCTTCAAATTGTTGCTATATTTAAAAATATCAATAAATTTAATTGAACACAAATTGAACACAACAAAAACATTTAGTATTAAAAACATTGATAGGAGAAATTTATTTATGAAAATAACTATTATTTCAGGTGGATCAGGTAATGATGCACTAGTAAAAGGATTAAAAGCTTTTTACAAAGATAAAAATTTAAGCATTAAAGTTATTGTAAATGCTTATGATAATGGAAAATCTACTGGTGTATGTAGAAAAGTTACTGATACTTTAGGAGTATCAGATATAAGAAAAAATCACTCAAGAATGTATGTGAGTGAATATGGCGATAAAGTGGATAAAAACCTTTTAGAGTTTTATGAAAAAAGATTTGATTTCACTAAAAATAAAGAAGAATTAGAAATTTCTAAACTTTTAGATAAATGAAATTTAAAAGAATATAAAAAATATGTTTCTAACTTTTTCAAAAACCCTAAGGCCAATGAATTTAATTATAAAGACTTTAGTGTTTCAAATATAGTATATTCTCAAATGTATAAAGAATTAGGTTATGAAAAAACAAATAAACATTTTTGCGATAAAATGGGAATTGATGATTTCGTTGTTTTAAATTCATTTGATAATATCTTTATCAAGGCATTAACGGAATCTGGAACAATAATAGATGATGAAGGAAAAACTGTCTTTTGAAACAATAAAAACGATAGAATAATTTCCACCATATATGAAGGAAAAATAAAATATGGTTTAAATCCTAAAGCTATTGAACTTATTGATAATTCGGATTTAATTATTATATCTACTGGTACTTTTTGATCGTCAATTCAACCTACAATTGAATATATGAATTTTTATGATTATATCAATAAGGCAAAATGTCAAAAAATTTGAATCATTAATAATGAAGATGATGGTGATTCATTTGGAGTTACTAATTTAGATTTTATCCATTATATGGAAAGAACCGGTCTTGACTTATCAAATGTAAAAATTTTACTTAATAAAAGTGCAAAAGAAACCTTAAAATTAACCATACCAAACTATAATTTCATAGTCAAAGATATGGGAAATATTAAGGGTAAACAAGAACCAGAAAAAATGGTTAAATCTGTTATTGATACCTATTATGATAAATAATTAAGGAGATAGATGAAATTCGGAATTATACTATCTGCTGGTAATCAAACTAGATTTAAAAGCGCAACTCCTAAAGCCTTGGTTAATGTAAATGGAAAAATTTTACTTGAGGAAAATATTTTGCGAATGTCAAAATATTGTGATCATATTTTTACGATATGTTCTTCCCAAAATGAGCATTTTTTTGAAAAATATAATCATATTGCTATCTCATCAGGTAAGGGTAGTGGAGATGCTGTACTAAAAGCATTATTGGAAATCAAACAAAAATATGGTTTTTCTTCAAATGATACTTGTTTTATATTATGAGGAGATTGTTTAATTGAAGACTTAATTTATAAAAAAGTTGTAGAAAATTATAAAAAAGTTGGTATTGTTCCATGTGTATTTGAAGAAAAACCCTACGTTCAAATATTTCAAAAACAAGATAATAAAATTGATGTTTTATTTTCTAAATTTGGAGAAAATGCATCATCTGGTTTTCATGATTTAAGTATTTTTTATTTTAATTGTGAGGAAATATTAAAATATCTTTTAGATTTTCAAAATAAAATAATAGGTATGGATGGAAATTATGTTCATAAACATGGAAATGAAATGGAATTTTTAGATGTTTTTAATGAAACAGATATAAAAGCTCAAATATTAGAAATCAAAAATTATCAAGATATGTCATTTAACACTATAGAACAATTAAAAAATATAAAATAGTTTTTACAAAAACATCCCGTAACTTTCGGGATGTTGCTACATTGTAATTTATGAGTTTATACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATCTTTTGTCCGCTAGGACAAATAAAAGTGAGGGAATTAACCCTCCCTAAAATATTTGGCAACTTGTCGCTCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCATACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGAAAAGAAGCATATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTCAAATTTCATGGATTTTTATTAGGAATTTTTACCAAAAACCCATATCTAGAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTGCCTTTACTCTTGCCTATAATAAGGTCTATTTCCCAATGGCCGAATTCTTTTCTTTCGTTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTACGCCAACCAATCTTCTAGCAGCGGTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTGACAGTTCACAATTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATCTAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTATTTCGTTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGCTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCTTTGGGTGATTTTTTTTATAAAAAAACTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAGTGAGATCTCTAAAACCTAGGGGTCTCACTTTTGGAATTAACACCGAGATCTAACTAAAATGTTTTGGTTTCTTATTGAAAGATTTTTATATATAATTACATTTATGATAAAAATAGTTAATTTATCAAAAAAAGATAAAAAAAATCGATTGATTTTAAACAACTTAAATTTCAATATTCATGAAAATGAATTGCATGTTTTTTTGGGCAAAAATGGTGCTGGAAAAACAGTTACTTTAGGAATAATTTGTGGATTAGATTCAGAATTTTCTGGAAAAATTTTAAAAGATAATGTAGATATTTACAAATCAAATAAAACTAATAATTATTTGTTTGTTCCAACAGATATTAGATTTCCAAATCATTTAAAAGTTATTGATTTAATTAAATTTTATGTGAATGAAATTTTTGAATTAGAAACAAATGAATTATTTATAAATGAAACATTAAAAGAATTCCATTTAGATGAAAGAAAGGACTATCTTCCTAAAAAATTATCTTCTGGAGAAAAAAGAAAATTAATGCTATTAATATCTAAATTAGTAAAACCAAAATATTTATTCTTAGATGAACCAGAAGCTAATCTAGATCCTTATTCAAGAAATGAATTATTTAAACTTTTGAATATGTTAAAAAAAGAAGGAACTTCAATTTTCGCATCATCTCACTTAATATCTGAAGAATTGCAAGATATAGATAGAATAACAATTTTGCATAACAAAACTATCGTTCTAAGTGAAAAAGTAAAATCAGCAAAAAATTTAAAAATTATTTTTGATAATTTTAAGAGAGGTGGTTATGACTAAAAAAATACTATCTCTAACTTTTAAGTTTAATAAGAAAAACAAATGAGTATATATTGGTTTATCACTTTCTTTTATAATCAGTTTTATATTTTGTTCTTTGTTCTTATTTGATAATTATCTTTTTTATAATTATTTTTGAATGATTTTTCTATTGGATTTTATTATAGTTTGTGTGTCAACATTATTTATTCTGCATTTAACATTTGTCTCAAACCAAAAATCGATATATGAAAACACATTAAATTGTAGATATTTTAATAAACCTAAAATTGTTTTAGCTTGGTCAATATATTCATTTATTTTATTATCCGCATTTTCATTATTAAAGTCTTTTGCAATAAGTTCATTGCATTTATATAGAGACACGAATATAAAAAGTTTTCTACTTTATTTTATAGGCTATTTTCTAAGTAATTTATTGATAATCATTTTTATTATTTTAGTTTTTGCAAGTTTTATATTTATAACAAAATCTTATAAAAAGTCTCTCGTCATTGCAACTTGTATATTACCTTTTTTTACTGTTGTGTCTTTATCATCAAAAACAATAGAAGCGCGAGCAAATAAAAAGGTAGAAATAAACAAGGATATAATAGTTTCTGGTGACAAGTATTACTTTTTATCAGATGATGAGATTAAAAGTAAATATAATTTGAACTTAATTTCATCTATATATTTTTTACCCAAAAATACTATAAATAAGTACTTTGATGAGAATTTCTTGACTTATAAAAATAATTCAGATACAAAAAATTATATATTCTCAACATTCAATTTGTCTATTCTAAAAAATAATAAAAAATTTGACAAAGATAAATTTAGAATTTATCATAACGATGGTGAGTTTTTTATAAAAATGAGTACAAATGAATTGGCTAATTATTTATATAGCAAATTAAAAAAATATTTTGAATCAGGTGGAGTTAATTTAGATAAATTACTTAAAAAAATAAAATCTAATATATGAATAAAAAACAACTTTACTGGAAATGAATACAATTTAGTAATGCATTTACTTGGCAAAGATGATTTTAATTTTCAATACTTGCAAAGAGACTGAAATGATAATTTATGAAATGCTGCAAAATTAAAGTCAAATTTAAACAGTATGTTCAAAATTAATTTCTTTGATTTTTTAAATCATTTATACAGATCAAATCAAACAAAATTTAACACCACAATTAACATGGCTGAATTTTATATTGTTAAAGATAATAATTTAATAAAATACTTAGATTCAAATAGTAAAATAACTGATTCAGATTTAAATTTTATTAGCGAAAAAATGGTGAATTTTGTTGGTGATAAAGTTATTATTGATTTTAATAAATATGCACAAAGTGAACTTGAAAATTCAATAACAACAACAATTACTAAATTCAATTTATATTTTCCTGAAATAGATTCAAAAGAAAAATGAAAAAATCTTGTTTATAGTTCTTCAAATCATTTGTTCATTATTAAACAAACAATTGATAAAATGAATGAACTTGTATCTAATTTAGGAAATTATCAAGCGTTTAAATTTGAAGAAAACGTAAATCCCAAAAACTTTTATGACAATACAAAAATAATAAAACTAAATAATTTGACATATGACCTGATAAACTTATTTTTAATAACATTTGTTATTTTAACACTCAGCATTTTGCCAATAATATTTACTTTTAAAAGGAGAAAACATGAATAAGAAATTAAAAATTAATTTACTATCTATTTCAACAATAATGATGGGGGCATCATTTGTTGTCGCATCATCTTGTGGTTCTAAAGAACAAGAGCAAGAAAAAGTTTTAAGTGATAATGAATATGTTGAGAAACACTCTATAACAAGAGACACAAATATTAAGGAAACAAGATATATTAGTCAAAATGTAAACAAAGTTTTTGACAACCACATACAATTAGAAAATTTTGTTAAAAATAATATAAAATCTGTTGATTTGAAATTTTTAAAAAACTCAAAAAAATTTGATGATATAAATAAAAATAATCCACAATTTATGTCCAATGAAGAGCTTTCATATTTTGATTTTAGCAAGTCAACAAGTAACGAAAAAATGCCGATATTTTTAGGACGAAACGGGAAAGTTTTTACTTCATTAGAAAAAGCAAGAGATTCATATTTTAATATAAATCAAGTTTACAAATTAGGCAATATTTATGCAAACAGTAAAGAGGAACTTTACAATTTCATTTTAAATAAATCAGTAAATAATAAAGATTTTTTGATAGAAGTATTAAATGAAATAAAAAGCAAATTGAATTTTAATTCACCTAACGCAACTATTACAGAATTTTTCAAAAGTCCAAGCGGACAAATTACAAGTTTTAAAAAAGAAGACCTTAAAGATGCAGACAAAAAAAGCAGAATTGAAGATTACATTAAAAATGTTGCAAAGAAATATGTAATCTATTATGATGAAGAAAATAATCCTCACTTAAAAGCGGTTGAAGATTATATATTTGATAGAATATCAAACTCTGAATTTATTAAGAGTAAAATCACTAGAATTAATTCTAATAAAGGATATAACAAATATTATGTAGACAATTCCAAAGATGATGAATTTACTTTGTATGGGCCAAGAATTTTAGAGACTCCTTTTGAAAATATTGAAGAAATAACTGATACAACTAAATGAAGTTCAACAAATAATCATTCTAAAGAAGAAATATTTAATTGATTAAAAATACAAAAGATGAACAGTATTGTCACATCACTTTTCAAAATTGTGTATTTTAATGAAAACATAAAGAAAAATGTTGAAATTACACCATTTACACCTATAACAATAAATGTTGAAAAAGATCATTTAAACAATTTCAAAAAATGTACCAAAAACTTTTGTTTTGAAGGCACTAACCTATACGATTATTTATATAACAAATATGATCTTTTAGCAAAAGGAAAAGAATCTAATTTTCTTTCTAGTTTTTATATATTATATGTTGCGGGTTTAAACAAAATTATAGAATATAAAGCAAATCAAAAAGTGGTTGATGAATATAAAACATATTTTAAAATGATCATAAATGACATTAGCAAAATTTTTGCATCATTATTACCAAAATATTTCATTCATGAAGGAATTAGTGAATCAGAAAACTCAGATAATCAAGTAAATATATCAAAAATTTGAGATATAACAAACGGGGAATTTGACTTAAATTCAGATTCAAATATATTGATTGCTAAATTATTGAAATATAAAATAGCATGCAATTTTTTGGATGTAATTGCAAGTGGCATATTTAATGCAGTATATAGATTTTCAAGTGAAGATTATGATCCAGAATCTCTCATTTTTCCTGAATATGCAATATATGATAATTATGAACATTATTTTGAAATTTATAGTTTAAAGCGATTTAAAAAACCAGTTTTTTCAAAAAATTATGACAAATTTACTAAACAATCATATGATACTAACAACAGCTTACAAGAATGATTTTTAGAAAAGTTAATGTCGACAGTAAAAAACAACTTTGATAATGTGAATGAATTAGTAAAAGATATTCTTCTAAATAATGAAGATTTATTAAACAAAGATAAAAGTTTTTGATTATTAAACTATTATAATAATGAAAAGCTTCAAAATGAAAATTTATTTACTTTTATATGTAGAAAATTTATCACTATTCATCAAAAATATGAACAAATGAAAAAAGATTTGTTGCCAGTAGAAAAAAAATCATTTGCTAAATATTTATTATATGCTTTCATAGAAATAGATAATATAGTTAAAAAAGGTAGTTGGTCAAGCAAAGTTAATCTTTTAGTAGAAACTACAAAATATATTCATTCAATAATAGATGCAAAAGCAGCAGCATTAGAAATAGCTTTAAATGTTGTCGGAGGTCCAGTTTCTGCTTTTGGTGGTTTATTGTCTAACTTTTATGTTAAATTTTACAAAGCATTATTTGGTGAATTTAGCCAATTTAACTATAAATACGTTGTCAATGCAGGAGATAAAGGTAATGTCGATTATATATGGAATGGTGGATTGCGTTATGTTGGTTTATTTCATTGAATAAATGTTGAGTCTGGAATACAACATGCTAAAATATTACCACCTGTAAAAATAATGGACGGTTATACTGAAAGTTTTTATTACTTCAATGGTAAAAAATATAACTTTGTTGATATAGATTTATTAAAAAGAGATGTTGTAAATTACTATTTAAAAGGAAACAACAAAATTATAAACAACTTATTAGAAAAAGATGAAGCTGAAAAGAACATAAAAATTATTTACTCATGAGACAAACAATTAGAACAAAAAATTTCATTTAATGATTATAATAACCCAAAATTTTTAAGAAAATATAATTCTTGAGCTAAAAATGATGGGTTTAATATAATTGAAGCAAATTTTGGTGAACATGAAGATTCTTGATTTTCGTCATTAGTTAATTATAAAAACGGCACACCATTTAGCTTAGCAAAAGAAACAGACGATATAAAAGACATTTTAAATTCAATTAAACAAGCGATCGACATTTTGAATCCTATTTATTATTCAAAAAAACCAATAGAAACAGAAGATGGTTACACCGACAAGCTATTTCCTATTGATCAATTTGATCCAACAAAAAAAGCTTTTGAAGACAAAAAAATACTAGCAAATATTTATAATGAAGATCCATTAAAAAATTGCTTTTTATATTCAGAAATAGGAAACTTTAATATTCAAAGCGATTTGTTAAAAAATAATAGAAACAAATTTATTCCATTTTTAAATTCATTGAATATAAGAACAAAAGAAGTTTCAAATATATCATTTTTTAATAATTCAAATTATCAAGATATGGAATTGTTGCAAACAAAAACACTTTATTATTTTGATTATAAAAACAATAAATACTTCTTTACAAATTTAGACAAAGCGAGAAATTATTTTATCAATGTTATTATTAACCCAAAAATGGAATTATATAATTTAACCAAAAGAACCAAAATTATATTTATGAATAAAGAGTTCTGTACATTGAATGAAGCAGTATCTTGATACAAAAAGAATAATAAAAGAGGCGAAAATGAAAATTAAAAAATTATTTTTATCTTTAATTCCAACAGTTGTTTTGATTCCATCATGCAATAATTCAGCCTCTATGAATTATTATATGCAAAACAATAATTTAAAAAACGCAAGCAAATATGAAATTAATTTAGAAGGACAAAAATACCAATATACTGATATTGAATCATTTATGTCTAATTTAATAAATAGTAATAAAATAAAAGTTGAAAACAAATTAGTAATAGGCAAAAAGAAAAACAATAGTTCAAATAAAATTCTAGATTCTACTATTTTTTATAATTTCGACCCAAATAAACTTAAAAAAGTTTATCAAAATAAATTCCAAAAAACGGTTTATTCAGTTGATGAAGTAATAGAATCGTTTAGAGAAGAAAGCTCATATATTAAAAAATATCTTTTTGCCAATAAAGAATTTGATTCAGAAGAAAAAGTAAAACAGTATATAAAAACTCAACTTAAAAACGGTTTTAATAAATATGTTTATTATTCTAATAATGGGAAAAAATATAATGCTATGAATTGGCAAGATTCATTAGAATTGCTAAAAGATAAATATAATAAAGATAACAATGTGTTTTATCCTATTTTTGATAACAACAATAATGTAATTTATAATAATTTACTTAATTATGAAACAATGAATTCTATTAGTAAAAAAATTCTTGAAAAGTTAATGAAGTTTAAAGTAGAAATAAAATTTGGTTTAGATCTCAATGATGAAAAGTCTTATGACTACAATGAAAAGGATTTAGGTTTTAAAGTAAATGTAAAAAATAATAAATGAAATGTTTATGATGGCAATAAATTGCGCAGAACATTCAATTTAAATGAATTTTACAAATTTTATAAAAATGGAATGAAAAATCATAACGGTTTTTGAGATGCAACTTCAAAAAATGTTGAAAATTATTATGACCACGAAAAAGGAAGATTTGAAGATTGAACTTGGATTGTAAAAGCCAACATATTAAATTTATTTGATACAGAAAATGTCGAATTAATTTTCAATGATATAGCGATGAATAAACCGTATGTAGGCACTATTTGATTAATTGATGCAAATTATACGATTCATAAAAAATTAAAAGTCAAAACATTTGTTGGATATGAAAAAGAGCACTATTATTCAAGACCAGTTAATTCATTTGGGCCAAGAATTAAGGCTTATTATGACCAAAAGAGCACATTTGAAGAATTAAACAACATTGACATTAACAATTTCTTTTCTACTTATCAAATTTCAAATAATAATTTTGATTCGTTATCAATTGAAGAAAAAGAGCAAATTGTTACAGTTTATAAAAAATATATAGAATATATTCTTAAAAATGATGAATGTTTAAATCAAATAAAAAATTATAATGTCATAACTTTTGATAGTTATATGAATAAAAATTTGATAATAAATGGTAATGACTATTCAAAAAAAATTGATTTAGACAAAATAGATTGAGATGATTATAAACCAATTAATTTGAATAAAATATGAGAAGAATTTGATTTTAGTTTTTCAAAAATAAATGATATTAAATTAAATAACAAATATATTTATGATAAATTTTATGCAATTACAGGAAATATTGGATTAATAAATGATGATAAAAGTCAAATTTTAACTGCCAACAACCGAAATTGTTCTTTATATGAAAAAAATAAAAATTCTAATATTTCAAATTTGATTTTTCAAAGAAAAACATGTATAGAATTTTTAAACAAAATAATTTCTTCTAAATCATTTCCAAGTGCTTCTGAACCCGAAGACAAACAAAAACTTTTAAAAGAAATTATTGAAGAACATAAGAATAAAAACAAATTAAATAATAGCTTTTTTGAAAATAATTTGCCCTTACTTATTAATGATAAAAACATTGAATTATTTTCTTTCTATTCAAATAATAATGTTGACATCTTATCAAAAAGATATTCAATACCTTCTAACATACTACTTGAGTTTCCAACTTTTTATTCTAAAGATGATTATGAAAATAACATAGCTCTTTATTTTGACGAAAATTGACACCCTGCATATATTTATCGTTTATCTTCATATACCTTAGAAAATAATGAAGAATTTCCAACAAAGGAAATACTTGATGGGAATTTGAGATATTTACTTAGTAATCAATTAGGGTATAAAGAAGATTTAGTTGCATATGATAATAAAGGTGTAGTAGAAATAATTAACAATGACATTTTCAATGTGTTTGTATTGAAATTTGAAGGCAATGAGTTTTCATTCCTGAGTTATAATGATGCAATTTCATTTTTAAAAGATTACATAATAACAAGATCAAAATTAATATAAATTTAAGATGGCAACATCTTATTTTTATATACAGTATATAACACTTTGTTTTTAAAATAACCAAAATAATATAAAATTAAAACACTATGGGATTTTTTGGATATTTAAAAGAAAAACTTTTTGGTTGAAAAAAGAAAAAAGAAGAAAAGAAAGCAGAAAAAGTTGTTGCTAAGCAAAAAGAATTAGAAGAAAAAGCACAATTAGAATTAATAACTTCAAAGAAGCTTGATAAATATGCTACAGGTCTCTCTAAATCTTCTTCTCTAGGTAAACAAATTTTCAACCTTCAAAATAAACATAAAGAAATAAATGAAGAATTCTTCGATGAACTCGAAGAGATTTTAATCATGAGTGATATTAATGCTAAATTAGTTTATGCAATTATCACACACATTAAAAACGAAGTTAGAGCTAGAGGTTTAAAAGATACTAAAGATATAGGTGAATTAGTTGCTGATCAAATGTTTGTTGTTTATACAAACAAAAGTATTGTTGATACAACTTTAAATTATCAAGACGGGAGATTAAATATCTTTATTTTTGTTGGAGTTAATGGTAGTGGTAAAACAACTTCAATTGCTAAAATAGCCTATAAATATATAAAAGAAGGAAAAAAAGTTTTAATTGCTGCAGGCGATACTTTTAGAGCTGGTGCAGTAGATCAATTAGGTGTGTGAGCAGAAAGAGTAGGCGCTCCTATTGTTAAACCTGTTAAAGAAGGAGTTGATCCTTCATCAGTAGTTTATGAAGCTCTTGAAAAAGCACAAAATGAAAAATATGACTTATTATTAATAGATACAGCTGGTAGATTACAAAATAAAGTCAACTTGATGAAAGAACTTGAAAAAATGTATGGCATTATTGCTAGATTTCAAGATAAAGCGCCACATGAATGCTTATTAGTTTTAGATGCAACAACTGGTCAAAATGGAGTTAGCCAAGCTAGAAGTTTTAAAGAAGTTGCGGCTCCAACTGGAATTATATTAACTAAAATGGATGGAACTTCTAAAGGTGGAATTGTACTTTCAATTAAAGACGAATTTGATTTGAATGTTAAATATATTGGACTTGGCGAAGGGCTAGATGATCTTCAAGAATTTGATTTAGATAACTTCATTTATGAAATGACAAAGGACTTAATTGTAAATGAATAAAGATATTAAAGATATAGAAAATAGAGAGCACTTATTAGAACTATTTGATAAATTTAATTCATTTTTAACTCAAACACAAAAACAAGCTTTTCAATTATATTTCATTGAAGATCTTTCTTATTCTGAAGTTGCCAACATTACAGCAACCACCCGTTCTGGAGCTTATGATAGTGTGAATAAAGCAATTAAAAAATTACTAGATATTGAAGCAAAAATGCAATAAATTAATACCATAAAACTGGTATTTTTTTATTATAAAATATGGAATTGTGTGAAATTTTACCAAAAAAAATATGGAATTGTGTGAAAAAACACATTTTTTATATATAATAAACATGAGGCAAATATGGCTTTATTAAAAAGAAAAATTGATAAATTTTTACAAGATTGAAAATTAAATAAAGAACGAAAACCTCTTATTATAAAAGGAGCTCGTCAAATTGGCAAAACGACATCTATTTTAGATTTTGCAAAAAATAATTATGAAAATGTTGTTTATATAAACTTTATAAGTGATTTAAGATATCAAGGTTGTTTTCAAAAAGGCTTTGATGTTGAGACAATTGTTAAAGAGCTTAGTTTTGTAAATCCAAATTTTCAATTTGTAAAGAATAAAACAATAATAATTTTAGATGAAATACAAGCATATCCGGATGCTTCAACATGTCTAAAATTTTTCGCGTTAGATAAAAAATTTGATGTTATATGTTCAGGATCTTTATTAGGTATTCAATATCAATACATTACCAGCAATAGTGCTGGATACAAAAATGAATATACAATGCAATCTTTAGATTTTGAAGAATTCTTATGAGCAAATGGATATAATGAAAAACAAATAAAGCAAATTTTTAACAATATGAAAGAACTTAAACCTTTCAGCGAAAATGAATTAAAAGTGTTATTTGAAAAATTTAATGACTATATTATTGTTGGAGGAATGCCAGAGATTGTTAAAAATTTCATAAAGCAAAAAAATTTTTCAAATATTTTAAATCAACAAAAAGAATTACTTGAAGTTTATAAGCAAGACATTGGTAAATATGCAGAAAGTTTGGATAGATACAAAATTTTAAAAGTTTATGAAAGTATTTCTCCATTTTTAGGTAAAGAAAACAAAAGATTTATGTTTTCAAAAGTTGAACATAATGCAAGGTTTAGAGATTATTTTTCAACAATGGAATGATTAAAAGATAGTGGCTTAATTAATATTTCATACTTATTAAATAATGTAGAATTTCCATTTTTAGCTAATTATGATTCAAACCAATTTAAAATTTATCACCATGACACTTCTTTATTAATAGCTTCTTTAGATAAAGAAACACAAGATGATTTAAGAATTAAAAAAAATTTAAATACTTATAAAGGCGCTATTTATGAAAACATAGTAGCTGAATCATTAAAAAAACAAGAATATGATCTTTTCTATTGAAAGAATCCAAAAAGCACTATTGAAATTAATTTTATAGTTAGAGATATTGATTATATAGTCCCTTTAGAAATTAAATCTAATAATAAAAAAGCAACAAGTCTTAAAAACTTTTTAGAATTATTCAAAAATAAAAATGTTAAATATGGAATTAAATTTGCTAACAGCAACATCGGATTTAAAAATAATATTTATACCTTTCCATACTTTTGTATTTTTATGCTAAGAGATTTTTTAAAAGATTTAGCTTTAAAAAGAAAATAAAAAAGCCATTTAAGGTTTCCAATTTAATTGAGGGTGAGAAATCGCTCTTTTTTATCTTTTTTCAATTATTAACATTTTATGAAAGTTTAGTTCATCGCCTTTATTTGCTCTATAAAGCGTTTCACTGATGCAATCAAAAACTAAACATTTTCTTTTTACGATATATTCTTTATTATCATTAAATAATGTAATTCTAGTTTTTTTATTGTAAAAAACAATACAATCTTCCTTATAATATCTATTCATTGATGTAGATGAATTTTCATTGTTAATTTTATTTAAATCTTTAAAATTTTTTACTTCAGTTTTTTTAGTATTAACTGATATTTCTAATAATCTTAATTCATCGTCTGAAGTATAAGCAGAGTGTACAAAGTCATTATTAAAATTAATAAAAGATCTATTTTTTAAATTTATTTTTTTAAAACAATTTTTTTCCAAAACATCTGTTTTATAGTTTTTTAAGCCAAAAAGACCATAAGATGAATTGTTTGTTGTTAAAACATACCAAGCTTTAGATGATTTTTCATTTTGTTCATTTTTTAAATAACTTATTTTCATTAATTCATTCTTTTTATTTAAGGTATGCAAAACTATTTGAGGTTCATATTTATCTAATTCAAAAATTTTACTGTTACCAAAATTTGTTAAGTGTTCATTGTAAGAAATTTTGTTATTAGCTTCAAAATTTTCCTCATCTTTAATGTCATAAAGAATTCTATCAATTGATAAACCTTTTTTGCTTACTTCATCAATGTAAAAATGTAATTCTGGAACTTTTCTTCAATTTAAACTTTTAGCCAAAATTGTTCTAATGTATCCAGAAGCATTTTGTAAAGCTTCTAATCCTCTCTTTTCATTTTCGCTAAATGACATATAGATTTTTACATGTGATAAATCACCTGATAATTCGCAATCAACCAATGTAGGATTAATAATATTTGTATTTGTTAAATCATTTGTTATTATTTCTGCTAATAATGTTCTAATTTGTGATTCTCTTCTTAATGTGTTTATACTTTTAGGCATAATATCTCCTTTATTCGCTATGAGCTTTATCTATTAATGATCAATTTTTAATAAGTATTCTTGCTTTTTTAAGTTTACTTGTATTTTCCTTATTATGCTCTAAAACAGCTTCAAATAATAAATCGGTAATTAACATTTGAGCTATTTTTGAACTTGAAGGAACTAACATAGTTGAACTTTGAATTTTGTGATATCTTAATTTAACTGCAATTAATTTATCTTCTTCTTGGCTAGAAGTTAAAACAATAATTGGCGCTTTTTGTTTTTTAGCTTGCTCGATAACAAAGTGAGCTTCCATTGTTTGTAAATTATTTGAGTAAACAATTACAACATCATTTTCAGTAACATGAGCTAAGGATGGAAAGAAAATATGAAAGTCACTATGAGCTATAACAGGTTTACCAATTTTTAATAAATTAGAAACTAAATTCATTGATATTCTTTGACTTGAACCACAACCGTGAATATAAATGTTTTTTGATTTATTAATTATTTCAGCTGCTTTGTTAATATCTTTTAATAGATCTTCATCATAAACATTATCTATTGCATATCTATGACTGGCTGCAATAAATTTATAGTCATTATCTACTTCATTGTCATTTCAAATAGTAGGATAAGTTTTTTCAAATTCTGTTAATTTGTTATTTATTGAAGCAATAAAAGTGCGATAGTCTTTAAAACCATATTTACGAATAAATCTAGAAACTCCCGCATCACTTGATTCAGTATTTTTAGCTAACTTATTTTGTGAAAAGTTAAAATAATTACCTTCAAAATTATTAATAAAATCTCGAATTCTTTTTTCACTTTTAGTTAATTCAACTGTATCATCCATTAATGGACGGCTTATTATTCTTTTGTCATTATTTTTCATAATTGTAATTATATTAAATTTTTTTAAAACTTATATTAAGCGTGGTTATAATAAAAAATTTATTTTTTAATTATGCTTACATTTATTAGTTTTGATAGTATCTAAAACATTTTATTTTTTTGTTTTGTATTTTTATATTTTATATTAAAATAATAAAGCAATAAGCTTGGGTGTCAAGTATTTGCACTTGACAGCAAGTTAAGTTATTGATAAAAAGAAAAATAAGGAGAAAAACCACAATGGTTAAAATTAGATTAAAGAGATTAGGAAGCAAATTTAATGCTTGCTACAAAATTGTGGCTGCTGACTCACACGCTCCTAGAGATGGGAAATATATTGAAGCATTAGGCCAATATAATCCACACTCAAAGGAATTTGTTTTAAATGAAGAAGCAACACAAAGATGAATTGACAATGGTGCTCAATTAACTCAAACCGTTTTCAATTTATTTAGACAACACAAATTAAATGTTAAATTCATGAACAACGCACACAAAGAAGCTACAAAAGCACAAGAAGTTAAAAAACTTGCAGCTAAACCAGCTACAAAACCTGCTTCAAAATCAACTGCTCCTAAAAAAGTAGTTGCTGACAAAAAAGTTGCAACAAAAGCAGCAACAACTAAAACTACAAAAGCTGCTGCTAAACCTTCAGCTAAAACTGTTCGTAAAACTACAAAAAAATAGTTATATCTAGATTATGAAAATAAATTTTTTAACTCTTTTTTCAAATTACTACAAACCATTTGTAGAAGAATCAATTATTTCAAAAGCTATTGAAAAGAAATTGATTGAAATTAATGTAGTTGATTTTAGACTTTTCAGCAAAGATAAACATCACAAAGTTGATGATGAAATTTTTGGTGGAGGTCATGGAATGCTCCTGCAAATAGAACCTATTGATTTAGCACTTGATTCTCTTAAAGAAAGAGGAGGTTACAAAATTCTTGTAACTCCTCAAGGAAAGAAATTTGATCAACAAATTGCTAATGAATTAGCTAAGAAAAAAGAAATAACTTTTATTTCTGGTCGCTATGAAGGCTTTGATGAAAGAATAGTAGACCTTGTTGATGAAGAAATTTCAATTGGTGATTATGTTTTAACCGGAGGCGAATTACCTTCAATGGTAATGGCAGACAGTATCATAAGATTAGTTCCTGGCGTAATAAGAGAAGAAAGTTATGCTAATGATTCATTTCAAAATGAAGGTTTGTTAGATTATCCTCAATACACTAGACCAAGAGTGTACAAGAGCAAAGCTAAAAAAGAAGCTTTAATAGTGCCTGAAGTTTTAGTTAATGGAAATCATGCAGAAATTGAAAAATGGAAAAAAGAAGCTCAATTAAAAAAGACAAAGAAAAATCGTCCAGATATAATCGAAAGGATTAAAAATGAGAAATAAATTAATCGAATTAGTAGAATCAAACCAATTACGTACAGACTTCCCAGAATTCAGAGTTGGAGATAATGTTAAAGTTCACGTTCGTATTCGTGAAGGTGAAAAAGAACGTATCCAAATTTTTGAAGGTTTAGTTATTAGCAAAAAAGAATCAGGTACACGTGAAACATTTACAGTTAGAAAAATTTCTTTTGGTATAGGTGTTAATAGAACATTTCCATTACACTCACCTATGATCGCTTCAATTGAAGTTGTTCGTTCAAATAAAGTACGTCGTGCAAAACTTTACTACATGAAAAACAGAAGTGGTAAAAGTGCTCGTCTTAAGGAAATTAAACGTACAAAATAATTTAACTTTTATTAATAATCAAGTAGCTCAGAAGGCTACTTTTTTTGCTGTTTTATAAGCTTTTTAGTAAAAAAAGCAAAATAAAATTTGTGCCTTTAACACAAATAATATTGATGTTTATAACAATTCATTATTTATTTTTATATTTACTTGATAAAAAACTCAGCCATCAATTCTAACATGAATTAGGTCGCCATCTAAATTATGACTTAATGAAGCATTTCTTTTCTTAAAAACATTTAATTTATCTACAGTTTTACCATCAACTAAAAGTTCAATATATGAAACTCCAACCAATACTTCAATAGTTTTATCTTTATAGTTAACAACTTTATAATTAGTTCCAATACTAAAATTAATAAATCAAATTGTTATTATGAAAAAAACAGCTGATATGATAATGGCTATTATTGAACCAATTGAATTTTTAGTTGTAACAACTATATGAATTATTGGAAATAATGCTCCAGCTAATCCAATACTTATAAAAAGAAATATTAAACTAGCAATTATCATTCCTGTACGTTTCTTTTTAAATTTTTTTCCTTTTTCAGACATATGTCCTTTCTTTATTAAATAATGTTTTTTGTAATTATTTATGATACTTTTTATTATTAATGATCATAAATGAACGATAGATTTGTTCAGCTAACATAACTCTAAACAATTGATGTGGCCAAGTTAATTTTGAAGAATTAATTTTCATATTGCATTTAATTAATTCTTCATTAACTCCATCACTGCCGCCAATTATAAAGGTTAAATTAACATTGTTATTAACTATTTCGCTTAATTCAACTGAATCTAAACTTTTTCCTTTTAATGAAGCATAAATAACAAAACTATTATTATCAACATAAGAATTAATTAATTTTGTTTCCTTGTCTTTCTTGACTTCGATATTTTTTTCCTCACTCAATTCTTTTACTTCAACAACATTTAATTCAGCAAAATGACTTATGTTTTTAGCATATTGATTAAATAAAACTTTAAATTCTGGACTAAGTGAACCAACTGCAATTATTTTAATCTTATGCATTATTCTTTTTTCTTGTAGTTTCTAAATAAAACATAAGCATTTGAATATCAGCTGGATTAATGCCACTGATTCTACTTGCTTGGCCGATTGTTGTTGGTTTAACTTTGATTAATTTTTGTTTAGCTTCTGTAGCAATATTATTAATTTTGTTGTAGTCCAAGTCTTCTGGTATTTTGTAATTTTCTAGTCTTACAGCTTTATTAGCATCACTTTTTTGTTTTTCAATATAACCATGAAGTCTAACCATTGTTGTTAATTCTTCTAGGTATGGAAAATCAGGAATGATATCATAAGGATCAACTGCAGGACGAGCTAAAGTTTGTAATAATGAGATACCTGTTGTGATTCCATATTTTATAGCCACAGGATCTTTTGAAGAAAGATAAGTAGTATGAAGTCTTTGAATTTCTTGGTCAATTAATTCATATTTTTTAATAACCTTTTGATATTGATGTTTGTCAATTAAGCCTATTTTATAGCCATATTCGGCTAAACGAATATCAGCATTATCATTTCTTAAAAGTAATCTATATTCAGCTCTTGAAGTTAGCATTCTATAAGGTTCTTTAGTTCCTTTAGTAACCAAATCATCAATTAAAACTCCTATATAAGCATGGTTTCTTAAAATAACTATACCATCTTTTTTTTCTAAGTTTAAGGCTGCATTAATACCTGCAATTAAACCTTGAGCAGCAGCCTCTTCGTAACCACTAGTTCCATTAATTTGACCTGCAGTATAAAAATTTTTAATGATTTTAGTTTCTAAAGTAGGTTTTAATTGCAAAGGATCAATTGCATCATATTCAATAGCATAAGCATACTTTTGAATCTTACATTTTTGAAGACCAGGAATACTTCTAATCATTAGTTCTTGGACTTCAACTGGCATTGAAGTAGAGAGCCCATTAACATAAGTAATTGAACCATCAGCAGTTTCAGGTTCGAAGAAAATTTGATGTCTTTCTTTTGAAGGAAACTTAACAATTTTGTCTTCAACTGAAGGACAATAACGAGGGCCAATACCTTTGATTATTCCTCCATACATAGCACTTTTAGTTAAATTTTCTTCTATTATTTTGTGAGTTTCAGGAGTTGTATAAGTTAAGTAACAAGCAATTTGACGAGGTAATTTTACATTTGATCTAGATGAAAATGATAAGTTAGTATCACTTAATACTTCTTGTTCTACTTGCGAGTAGTCAATTGAATCTGAAAAGATTCTTGGAGGTGTTCCAGTTTTAAGTCTTTGAATTATAAAACCATGTTCTTTTAATGAAGCACTTAATTTAGGTGTAGTTTTTTCATTATCAGGTCCACTGATTTTAATAACATCTCCTCTAAGAATTCTACTGTCCATATATGTTCCAGTAGTTACAACACAGACCTCAGCTTCAACTATATCGCCATTAGCTAGTTTAATTCCTTTAAATTCTTTTTTACTATTTACTAATATTTCTTCAGCTATATCTTCAATAAGATCTAAGTTAGGTTCTTTTAACACGTATTCTAAAGCTATCTTCATGTATTTTTCTTTGTCAATTTGAGCTCTAAGTGCTCTAACTGCAGGACCTTTTGATTGATTAAGCATTTTCAATTGGATCATGCTTAAATCAGCCCAAAGTCCTTGCATTCCGCCTAAGGCATCTATTTCTCTAGTTATGATTCCTTTGGCTGGTCCACCAATTGAAGGATTACATGGCATCATACCTAAACGTTCCTTATTTAAAGATATCAAAACAGTTTTATGATTTTTGTGTGCTAAGGCAAAAGCAGCTTCTAAACCAGCGTGCCCTCCACCTATAACAACAGCATCATATTTATGTACATTATTACTTTTCTTATTCATAGTTCTTATATTTTAGTTTATTTTTTAAAAAAATATATTATAATAATAAAGCAATTTTAATGGCGGATGTGGTGAAGTGGTCTAACACAGCGGGTTGTGGTCCCGTCATTCGAGGGTTCGAATCCCTTCATCCGCCCCATTTTTTTTATTCCTTTTTTCATATTTTTTTCATAAATTTTAAATAATTAAAATGCTGCTTATTTTACTAATTTAGCTATAAAACAGAGTTATTAGTCAAAACACGGTCAAAAATATTTTATTAATTAAATTTAAAAATTTAAATAATTCAATATAATTAAATATCAAACTTAAGGAGAAAAATATGAAAATAGGAAATTATAAAGTTGCATTACAAGCAATCGAAAAATATAAGAATATTATTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATAAAAAAGAATTATCCAAAGAAAAAAGTTTTTACAGTTGGAGATAATGTTCATACTTTTGATTTTATGAGTTACCACTATGACCCAATTGAAACGGTTGATTTTAAAAATTCATTAGGTATTGTAGTTGATGCTTCAAGTAGTGATCGGATTCAAGATGGACATTTATTAGTAGAAAAAAAATGTACAGCTGCTTTAAGAATTGATCACCATCCAAATGGTTCAGATATCGAATATGATTATTTATGGATCGATGAACACTATGTAGCTGCGGCTGAAATGGTGGCTATGTTAGCTAAAAGTGCAAAATGAGAAATCACTAAAAAAGCTGCCGAACATATTTATTTAGGAATAAACACAGACTCAGGTAGATTCTTATATCCAGATACTTCTGCTAGAACATATAAATTAGTAGCTCACTTAATTGAAAAAGGTGGTTTAGATTCTCAATGAATCTTAAGAGAATTAAACAAAAGAAGTTCAAGAGATATTAAATATACAGGCGAAATTCTTTCAGGATTCAAACAATCGGGCAGAGTTCTATATTTTGAAGTAACAAAAGAAATTATGGATAAATATGGCATGAATGATTTAGAAGCCGCAATATTTGTTAATGAATTAGCAAACATTGATGACAATTCTTGTTGAGCATTCTTTATTCAGCTTGCTGATGGCACAGTTAGATGTAGACTTCGTTCAAATGGTCCATTAGTTAATAATGTTGCTAGATTGCACAATGGTGGCGGTCATGATAATGCTGCAGGTTGCTCAATTAATTCTTGAGATGAAGTACCAGTAGTATTAAAAGAATTAAATGACTGCATTAAAGAATGAGAGGCAAAATAATGGGACACATGAAAATTGGTAGCTTAAAAGAAGCAACAAAATTAATTAAAAAATATGACTCAATAGTTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATTAGAGCTAATTTTCCAGAAAAGAAAGTTTACACAGTTGGCGATAATGAAGGTATGTTTCCATTTTTAAATCTTAAATATGATAAATGTCCTTCAGATAATGTACTTAAAAAATCTTTAGGCATTATTCCGGATGCAAATGGTAAAGAAAGAATTCAATGTCGAGAAATTTTAGATAAAAACTTATTTGCTGAAACACTTAGAATTGATCATCATCCAAATGGTGATGACTTAGATAAATGTACCAGATGAGTTGAATCAGATAGAATTGCAGCAGCTGAAATGGTAGCTGACTTAGCTTATACAAACAAATGAAAAGTAACAAAAAGGGCTGCTAATTGCATTTTCACAGGTATTGTTACTGATTCAGGAAGCTTTAGATATGGTGATACAAATGCCCGCACTCACCTTTTAGCTTCATATTTATATGAACAAGGTTTAGAGGCTGACAAAATATTCCTAGGTCTAGCAGCTACACCTTTAGACGATTTAAAAATTAATCAAACATTGTTAAATAATTTAAAAACAGTTGGTAAAGTTGCTTATACAACTATTGATTTAAAAACAACAAAAGCTTTAGGCAAAAAACCAAATCAAGTTGTTAGAGTAAATACAATTGGGAATATTATTGGTTATCCATTTTGGGTTCAATTCAATGAAGAAGAAAATGGCAATGTTAGAATTGAATTTAGATCAAGTGGTCCTTGTGTTAGAAATGTTGCAGTTAAATGAGGCGGCGGCGGCCACGAAAGAGCCTCAGGAGCTGTTATTAAAAGCTGAAAAGATTTAGATGCAGTTATTAAAGACTGTAATGCGGAAGTTGAACGTTGAGAAAGAGAAGGCAACGACAACCAAGAAAAATAATTTTAAGACTTGCACGAGTGCAAGTTTTTTTGTTGACTTAAAAATCTTGTGATATAATAAAACCATCAAGTGAATATTACAGAGTTGCAACTTGCAACTCTTTGTTATTTTTGTTGGAGGTTTCATGAATTGAAAAGAAATACTTGAAAAACAATTTGGAAGTCAAATTTTAGAAGCAAAAATTATTGAAGAAGATGACTTAAAATTGTTAGACATTAGTGTCAATTATCATGACTTAAATAAGGTTGAAGAAATATCAAAACAACTTAATGAATTTATAGATTCTAATTATCCATCTTTGGATATTGATTCATTATCAATACATTCTCCAGGTGTCGAATTAACCTATAAAACAGATGAATTAGGAAAACACATTGGAGAAATGTTAACTATAAAATTAAATAAAAGTATTGAAAAACAAGACAAATTTAATGGTAAATTATTAGAAGTTTTAGAAGGCAAAATTCAAATAAGTTGAAACAACAAAGGCCAATTTAGAAAAGTATTTATCGACAAAAATAACATAGTAAAAGTAGAAAAATATATAAAGTTTAATTAAGGAGAAAAAATGCCAAGAAAAAGTAAAACAGAAGCACAAGAAACTTTTAATGCAAGTAAAGCTTGATATCAAATTTTGATAGGTTATTTTGAAAAAGAAAAACTTCCTATTGAAGAAGTTATCAAAATTTTTTCAGAAGAAACTACACGAATTATTAACAAAATCTTAGATCCAAATGCTGAAATAGTTTTTGAATTAGACAAAGAAAAACAAGAAGTTCACATTTTTAACAACAAAACATGTGTAACTAAAGATGAAGAATTATTGCTTGATCTTGAAAATCAAATAAGTTTTGTTGGATATAAAGAAGCAAAAGTAAGAATGCCTAAAGTTAAAGAAGATGATGAAATTAAATGAGAAATTGACTTAGGAGTATTAGACAAAAGCAAAAATCCTGAAGCTAAAAAATCATTAAAAATTATCAACTCATCAATTGCACAAGCTATTAAAACTCTTCAAAAGAAAATGGTTTTTGAACAATACTCAAGCAAAATTGGACAAACTGTTAAAGTTGTATTGAATTCAAAAAATTCAAATGGTTCATGAAATGTTCAAATAGTGAACAGTAATGTTAATGCATATTTACCAGCAGGATTAATTAGTACAAAAAGAAAAGCAAACCCAGGTCAATATTTTGATGTTGTTATTGAAAAAGTTTCAGAAGAAACAAAATTGAGTCAAATTGAAGTTTCATTAGACTCACCTAAAATTGTTTGAAATATTTTACAAAACAATATTCCAGAAATTGCTGAGGGTTTAATTGAAGTTGTTGATGTAGTTCGTCAACCAGGAGAAAGAGTTAAAGTTGCAGTTAAATTAGCAGAAGGCGCAAATCCTGATATTGATGTTAGAGGTTCAATTATTGGTGAAAATGGATCAAGAATTGATTTATTAAGTCAAAAATTAGATGGTGAAAAAATTGATGTTATTCTATATGATGAAGATATCAAAAAATATGTTGCTAACTCAATTAGTCCAATTCCAGCTGTTGATGTTATTTTAAAAGATGAAAGAGAAAACAAATATTTAGTTATTGTTGTTCCTGAACACTTAACACCTGCTATTGGTAAAAGAGGGGTTAATGCTTATTTAGCTTCAAGTTTAACAAAAGTTAAATTAGATATTATTTCAATAACTGATGCTAAAGCTCAAGGAATAATTTTTGATGAAACATTAGTTCCTACTATTAGTGCAACCATTAAAAAAGAAGGACCTAAAATTAGATATCAAAAATCTAACAATAATAGAAGATTTAATAAGAAACCTGCTAACAACATTTTTGCTCAATCATTTGATTCAATCGATGTTTCAAACTTTGATCAAGATATTGCTGCTTTCGAATTACATGAACAAGACAGATTGGATTCATTACAAGAAGATGACTCAAACTTCGAAGAATTAGTTAAGAAAATTGAAAGCGAATATGCAGTTGAAAATGAAGTAGAAAACATTAAAAATGTTGAAGTAACAAAACCAGTTGCTCAAGATAACAAAATTTCCGCATCTGACTATAAAAAAGCTAAAGAAGTCGCAAAAGACTTTGTTGTTGATGATGATTTAAAGAATTTTGGAATTGACGATTTTGATTTAAGTGACATTGATTTTGATGAAGAAGAAAACAAATAATTTAACAAAAATTAATTCCAATTTTACTCGGAAGTGCATCATCACAGGTCTTATAAAACCAGTTGAAGAATTGGTTAGATTTGATTTTGATAAAAAAAACAATATAATTACACTAGATATTAATAGAGTCAAAAAGGGACGAGGTTGTTACTTTATACCTACAAGAGAGAATTGAGAAAACTTAAAAAAGCATAAAGGACTTAATCGTGCTTTTAGAACTGCTGTTGATCGTGAAGTATATGACAAAATAGAAAAAGAATTGGAGGACGCAAAATGCCTAGAAAGAACAGAATAAGTAACGTTGATGAAGTCAAGAACCAACTTTCAGATATTAAAACTGAAGTTAAGAATGGTGTTTTCATTTTTACAAACAAAATGCCATTAGGAGAATTTGCACAAAAAATTGGTATGAATGCCAATGATTTGATCAAGTGATTTTTGATGAAAGGAAAGCTTTATCAAATTAACTACGTACTTGATGAAGAAGAAATTGCTGAAGTATGTATGGAAAGAGGACTTGACTTTAGAAAAGAACAAAACATTGATGCTGCAAATTTTTTGCATGCAGTTAAATTTGAAGATGATGAAAAATCATTAGTAAAAAGACCTCCAATTATTACAGTTATGGGTCATGTTGATCACGGAAAAACTACATTAATTGACTTTATTAGAAAAACTAAAGTTGCTGAAACTGAAAGTAGTGGTATTACTCAACATACTGGTGCTTACCAAGTAGTTTACAAAGGCAACAAAATTACATTTATTGACACTCCTGGACATGAAGCATTTACTGAAATGCGTTCACGTGGAGCTAAAATTACAGACATTATTATTTTAGTAGTAGCTGCTGATGATGGTGTTATGCCACAAACTAGAGAAGCTATTGACCACGCAAAAGCAGCTGATGTTCCTATCGTTGTTTTTGTAAATAAAATAGATAAACCAAATAAGGATCTTGAAAGAATTAAAAGAGAATTAGCTGAATGCAACGTTATTGTTGAAGAATATGGTGGTGACGTTCAAATGGTAACTGGTTCTGCTTTAAAAGGTCAAGGTATTGAAGATTTATTTGAAGCAATTAGTTTACTTGCTCAATTATTGGATTTAAAAGCCAACCGTTCAAGATATCCAGTTGGTACTGTTATTGAAAGTAAAATAGATAAAGGTGTTGGTGTTGTTTCAACTTTAATTGTTGAAAACGGTAGCTTATATAAAGGAGACTTTATTGTAGCTGGCTCAAGTTATGGGCGTGTGCGTTCATTAAAAGATGCTACTGGAAAACAAATGGAACATGCTCACCCTGGAACACCAGTTGTTGTTACTGGATTAAATAATTCACCACTTGCAGGTGATAGATTTATTGGTTTTCAAGATGAAAAATTTGCTAAAAAATTAGCTGAAGAAAAAGCTCAAATCGATAAGAACAGAGCTTTATATGACAAGAGCCAAACTTCAGAAATTGAAATTGGCAAAAAGGTATTTAATGTAATCATTAGATCAGATGTTCAAGGAACAGCTGAAGCTATCAAAGGCAAAATAGATGGCATGTGTAATGATGAAGCAATGATTAAAGTTATTGGCGCTCAAGCGGGACAAATAACAAATGCTGACTTATTATTAGCTCAAGCCTCAGATGCAAGGATTATAACTTTCAATGTTAAGCCATCTGCAGCTATTAAGCAAAGTGCAAAACATTCGGGAATTACAATTGTTAATTATTCTGTTATTTACAAAATTATTGAAGATATGGAAGCTCACCTTAAGGGTCAAAAAGCAGTTGTTTATGAAGAAAAGAAAATCGGATCAGCTCATATTATCAAAGTTTTCTTCTATTCAAAAGTTGGAGCAATTGCTGGATGTATGATGGATGAAGGCGTTGTAAAAGCTAATTGTAAAGTTAAAGTTTTTAGAGGCAGAAAAATGATTCATGATGGTGTAATTGAAACACTTAAACGTGAATTAAATGATTCAAAAGAAGTTGAGAAAGGTAAAGATTTTGGTACTCACATTAAGAAGTTTGATGATATCAAGGAAGATGATATTTTAGAATTCTTTGAAGATGTTCCAGTATCTTTATAAGACTATAAAAAAGGCAAATAGGTAAAATTATGGATTTAAAAAAATATATTCGAGATATTGAAAATTTTCCTAAAACAGGCATAATGTTTAAAGATATTTCACCATTATTGGCAAATGGTAAAGCATTAAATTATACAATCACAGAAATGGCAAAATTAGCTCAAGATGTTGATGTTATTGTTGGACCAGATGCGAGAGGATTTTTATTTGGCACGCCTACTGCAGCATTCTTGAAGAAACCATTTATTATGGTTAGAAAACCAGGAAAATTACCTGGTGATGTTATTTCAAAAAGTTACGATTTGGAATATGGAAATAATGTTTTACAAATTCAAAAAGGGTTTATTAAAAAAGGTCAAACAGTTGCAATTATTGATGATGTTTTAGCAACGGGTGGAACAACTAAAGCTATTATAAAACTATTAGAAGAACAAGGTGCAATTGTTAAAAAAGTTATTTTACTTCTTGAATTAGTAGATCTTAATGGTAGACAATTAATCACTAAAGATAATGATATTGAAATAGTTTCCTTAGTTAAATTTTAAAAATAATTTATGTTACATAAATTTAAAATGTATGTAAAAAAGACCACAATGGTCTTTCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCGTCAGGACAAATAAAAGTGAGGGCATTAGTCCTCCCTAAAATATTTGGCGACTTGTTGCTCTTTCTAAAATATTATTTTAATTTTCATATTTTTTTGAAAAACAAATTTCTATTTTTGTTACTTTCTTAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAGTTTCTTCTTCATTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAGTCCATTTGAAAAGAAGCATATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTTAAATTTCATTGATTTTTATTAGGAATTTTTACCAAAAACCCATATCTAGAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTGCCTTTACTCTTGCCTACAATAAGGTCTATTTCCCAATGACCAAATTCTTTTCTTTCATTTATGTTTGTCGGTCTAGATCAAAAAGGTCTTACATAACGTGCACCAACCAGTCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTCCATCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTGACAGTCCACATTTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATATAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGTTATACTATAATTAGTTGTTCAAAGTAAGTATTCAAGAATAATGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCCTTGGGTGACTTTTTTTATAAAAAAACTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAAAGACCACAATGGTCTTTTTTAATTTTTATTATTTTTCAATTTCATCAATGCAAAATTCAAGTGTGTTATTAAGAATTCGAGAAGTAAAAGTTTTAACTTTGTAAGGCTCTCTTTTAAGTTGATCATGGTAGTAAGATTCAAATTCTTTTTTGTTCATGCCACTGAAGAAAAATGTAATTTTATTATTTGCGATTCTGCTTTCGATTATCTCCATTAATACTTGGAGTCTGAATCAGTAGTTATTTTTTTCTAAACCAATTTCGTCAATAATTAATGCATTAACTTTTTTGAGTTTGTTAATTATGTCAATAACTGCATTAGCATTTGTAGCATTGCTACCCATGTTTGAAATTAAGAATGAATATAAAGCACTGGTTGTTAAATAAACTGATGAAATACCTTCAGCTGCAAATTCGTTGGCTATTGCACTAGCAATGTAAGTTTTGCCAGTGTTGTTAGCTCCATAGACATAAATGCCTTTTAGTCCTTTTTGTTTCTTTTTAGCTATTGCAACTTTTAGATGATTAATATATTCAAACATTTCTTTTCTTTGAGCAGAAGAGAGGCGAATACGATCAAGTCTAGCTTCTGGATTAGGTTCGCAAATTTGAGTTAATCATAAATTGATGTGTCGTGTAACAACTGATTTGTATTCATTTTTTGCAGCAATCTTTTTAAAAACTAATTTTTCATTTTTTCTTGAAATAGAAAAAATCCATGGATAAATTTGAGAATTATCCAATGATTCTTTAATATTAATAAATGTAAATAAGTTTTCCAAAATTTCTTCATTTGTAATATTGTTAGCTTGAATTAATGCTTCTAGTTGCGGATGGCCATGCATTATTTCAAGTGCCTTGTTTTCAAATTCAGCCTTGGAATTAAAAAATAACTCATTTTTAATTTCTTCTTTTGCTGCCATAATAATTACTCCATTAAATATTGTTGTTGTCTTCTCTTTGAAGTGATTCTAAATAGGTAGCTTTATATAAATTAATTTTTGAAACTGCAATTCTGTTTTTAACTTTAAGTATGTCATCTAAATAATTTTCAATTGCTTCAAATGAGAAGATTTCTTTTTTAATTAAATCCTTAATAATTTTACCCACATAATTGGTATTTATGCAATTATTTATTTCATTTGAATAAAAGAATACTAAGTTTAAGCAAGGATCACCTAAGCCAGCTGCTCTACTTTCTTTAATTAAATCTAAAACACTTTGACGTGGTATTTTGTGACTAATTTGAGAATAGAAGTATCTTGAATCAGTTTTAAGTATTGCTTCATAAGGATTAGGATAATCAAAAGTATTAAGTGACAATTTGGTTTGAATTTCTTCAACTATTTCTTGAGTTTTAATCATACTTGAGTCAACCTCATGTGAAAAAGTAATAGTTAATTCTTCACTTGGTTCAGGAAAAACTTCTTCAAAAGAAGCTGAAGCATCCAAAAGATTTGAAGCTTTACTTAAATAAATATTTCTTTCACGTCCAACTAATCTTTCAAAGTTTTTGCTACCAATAATACTAATGAGTTTGTTTGCTAGAATTAAATTCTTTTTGAAACCTGTATTATCAAGTGGTTTTTCAATAACAAAAAGTGTTTTGCGATTAAACTCATCTATAAAAGTAGAAATTAAAGATACAGATTCTAATTTTATACGTGCTTGATTTAGTTTATTTACATCCATATTTAATAGATAAGTAAGGCTATCATAGTCATAAAAACCAGTTTCTGGAGATTGAGAAAATGATAGATCTCTAAGGTATTCATAAAGCAAAATTGCATCACTACCTAAAATAGGAGCGTAAAACTTGCGTAAGTTTTTTAAATCTTCTCCACTAACTGATGAAGAATGTTTGATTCTGAAGAATGCGTAAGGAATATTTTTTAGCATTTTTTACCTCCTATTTTTTTGGTCATTTTTGACCTTGTTTGCTCTTATTTTATGAGATAAAAAATAGAAGTTTTAAAAAATCTAAAATATTTTCATTATTAGTGAAGTTATGCCCATAGAAATCCACAATTGGTATAGACCCAAAAAGTTGATTTTTTATCGAAAAAGGGTTGTTTTTTTCATCTATTGCCTTAATTTTAGTTTTATCCACATTACGTTTTTTAAAATTTTTTAACCTATTTTTTTGGCTAGTTTCTAAGCACAAAACTGCAGAAAACAAAGAAATAAATTTAAAATTTTTTGGCAATAAATTTGGTATCTCAACAATGAAAAAATTTCCATCTTTTATTTCTTCAAAAATTAAAGGATAAATGATTTTTTCCAATTCTCCCAAATTATGTGCTTTTTGGTCAATTCAAAGTTTAATTTTTTCCTTACTTATACCATTTTTATCATTTAAAAAATCACCTAATTCTTGATTAATTTTGTGGTAAATTTTGCCATTTTTTTGATATTCACGATTAATAAACTCGTCACAATTAAAAGTTTTAAGACCTAATTTCTTAAAATTTTCTAAAAAAGTACTCTTTCCCACGGCAATTTGACCAATTAAAGCAATCATTTTAGGCTCCCTCCTTTATATATTCTTCTATAGCTAAGTTTGCACTTTTAATATAAGCTCTAGCTAAACCGTTGGCTCCCAACTTAATTCCACCAAAATAGCGAATTACAAAAACAACAACATTATTAATTTTGCGCATAATTAAAAGATCTCTAATTGGTCTGCCGGCTGTACCATTAGGTTCGCCATCATCGCTAAAGCCTGCATTGATCACTCCATTATTTAAAAAGCTATAACCATGACAAATATGAGTTGCTTTCTTGTATTCTTTTCTTAAATTAGCTTCTACTTCTTTTATTTGTTCCTTACTAGTCACATTAAAAGCTAAAGAATAAAACTTTGATTTTTTGATTTCATATAAAAGTGCTTTTTCCATATCTATTATTATATAGAAAGCTGAATAATAAAAAATAAAGTGGACTTACTAAGTCCACAAAAATTATTAGAATTCATCGATTTGATCGATTCTTGCTTGGTGTCTGCCACCTTCATATTCAGTTTTAATGTATTCGTTAAACATAGCTTGTGCTTCAGCAAAAGGTACATATCTACCACCCATAACTAAAACATTAGCATTATTATGTAATTTAGCAAGCCTTGCATCTTCAACTGTAACTACTCTAGCTGCGCGAATGTGTTTATGACGGTTTAAGGCATATGAAATACCTAAACCTGTACCACAAAAACCTAAACCAATTGTGTTTTTATGTTCATCAATATATTTAGCTAGGTTATGCCCTTGTTCAGCATATGAAATACTTTTTGAGCCATCCGCAGGTCCTAAGTCAACAACTTCATAACCTAAACTTTTTACATAATCGCGCATTTCATTTTTTAATTCGCAACCACCATGGTCGCTAGCAAGAGCAATAATATTTTTTTTAGCCATATTAGCCTCCTTAGTGTCTATTTTTAAATGATAAAGCAAAATTCTGGAAAAGTTTGGTAAAAATTTATCTTTTTTCCTAAATTTTTCCATTTTTTGTGTTTGCTAAAGAAAAAAAAGAAAGTTTAAAGTGCTATGAGAATAAAAAAACAAGAAGATATAAAAGACTGTGGTTTATATGTTTTGCAGTATTTTGTGGCAAAACATAATGATGACTACATTGATATTAATTATTTTAAAAATAAGGCAACTTACTCTTCAGAAGGAGTAAATATTTCAACACTTAAAAAATTAGCTTCTGAACACGATATCGATTTAAATAGTTATACAGGTGATTTTGAATCTCTTGAATCTTTGGACAATTCAAATATGCCTATAGCTTTATTATTAAATAGAAAAGGATATAGCCATTATGTAATTTTAGAAAAAATGAAGGACAATTATTTTATTGTTCAAGATCCAGCTTATGGTCGCAAGATGAAAGTTAAAAAGAAAGACTTGGCTAAGGAATTTGCCAATGTGATACTATTTTTTGATCCAAAAGAAGAAAAATCAAAAAATAAAAATAAAAACTATTCACTTGATAATAAAATAAGCACATTATTTTCATTTAAAAAATATACTTATCCTTTATTCATTTCAGCACTTTTAGGATTGTTTTTATCATTTGGATCAACATTTTTTATAAAAATAGTTTTTGATTTTATATTGCCTAATTATCTTAAAAATAATTTGATTATTATTTTTGTATTTTTCCTATGAATTAATATTCTGATGTTTATTAATCGGTTGTTTAAAAATCATGTTATTAAAAAAATAAAAAACGATTTAGAGTTTACTTTAACAAAAGCATTTTTTGACAAAATAAAAAGAGTGCCATCAAATTATTTATCTAAATTAACAAGCAATGATTACTTTAAAAGACTTACTTATATAAAAGAAATATGTGAATTTCAATCTAATTTTGTCTTTACTTTTTTAAGTGAAATATTTTCTTTTATAGGTTCAACAATCTTATTGATTTGAATTAATTATGTTCTATTTTTAATTATGTTTGGTATTGCTATTGTTGTTTTATTAGGTAATGTTATTTATCATTTTTGAATAGAAAAAAACTATCCAGATCTCTTGGAGAAAAATTTAAACAGCATGAAATCAGATATTGATTTTATATATTCCTGAAAAGAAATGGATAATGTAGATTATTCAAGATTTATTTCTAAAAACCAATTTAAAAACATGTTGGAATTTAAGAAAAAAGAATATTCATTTGCTTCTAAAGATGATATTAAAAACTTTTGAAATGATCTTTTTATTGGCAACTTTTCAACCATTATTGTTTTTGCTTCTAGCTTCTTTATTTTAAAAAATAAGTTATCAACTGGTTCACTAATGATGTTCTTAAATGGAATAAATTTCTTTGTTAATCCAATGCTAAATATGAGCTCATTATTACTTTCTCAAACAATGATCAAAAGAAATGTTAATTTAGTAAATTTTGTTTTAAATTTAGACGAAAAAAGCAATTTAGATACTGGTTTATTCATCGATAAAATTAAAAAAATTGAATTAGAAAATGTTAATTTTTATTACGAAAACGGAAAAAATATTTTAAATATTCCTTCATTTACGATTGACCAAAATATTCAATTAAATGGTAAAAATGGTAGTGGTAAAACCACTATGCTAAGTTTTATAAACGGCTGTTTTAAAGGTTATTTAGGAAAAATGAAGATTAATGATATTGATTTTGATTCAATAAATTTGATTAATTATCAAAGTAAAAATATATTGATAAATTCTTCCAGCCATTTACCAGATGTTTCTTTTCTCGAATATATTACAAGTAACAATGAAAATTATAAAAATGAATTGAATAATAATATTCAAAAATATGATCTATACAAAATATTTGAAGAAATGAATTTTAATTTAAATGGCAGCATAATTAGTAATGGAAGCAATTTGTCAAGTGGACAAAAACAACTTTTATTATTACTAAAATTATTTGTAAGAAAATATGACTTAATCATGCTTGATGAAGCCTTTGAAAATATTAATCCAAGTAATTGTGAATTATTGAAAAAGGCTATAAAGAAATATCAAGATGAAGCATTATTTATTGAAATAAGTCACAATAGGAACTATTTATTTAAAACAAAGGAGGTGTGCTTTGAAAAAATTAACAACTGTTTCTAAAACTGTGTGAATATTGTTAATATTGTTAGCTATTGCAACAGTAACTTTAATTGTTTTTATTTGTTTATATCATGTTGACAAAACAATAAGAGTTAAGTTACAAGTTAATGACAAAAAAGACTTAACTTTGTTAGCAAATGATAAGACTGCTTATTTATTAAAGAAAAATCAAAAAATAATAATTAATGTAAATGACCAAATTTTCAATTTAAAAATCCGCAACTTAAAAATTGAAAATCAAACGATTAAAGTAACCTTCTATAATTTGCCTAATAGTTTGAAACTTTTACCTAATACAACTTTAGATGCCACGATATTTGTGGATCGAGCCACTCTATTAAATTTAATATTAAATTCATAATTAATAATATAATTAGAATTATGGTTGAATTAAATGTTGTAAACAAGACTAAAAATAAATTTTTGTTTGAAAAAGAATACAAGCAAATATTAGAAAATTTAGCTAAAGAATTTGGGCTTAATCAAACTGTTTCAGTTGATGTAACTATTACAAATAATGATGAAATCCAAAAACTGAATAAAGAATATAGAAACAAAGATTATCCAACAGATATCTTGTCTTTTGGTTTAGAAGATTGAGAATTATTTGAAAATATGCCAATTTATCCCTTAGGTGAATTAGTAATAAGCCATGAAAAAGTTGAAGCACAAGCTCAAGAGTTTGGTCATTCAATTCGTAGAGAATATTGTTATTTATTTGCTCATGGATTAATTCACTTGATGGGATATGATCATGAAACAGAAGTCGAAAGAGCTGAAATGAATAAACTTGTTGATTTAATATTTGAACCATTAAAAATAAGTAGAGAGGAATAATCTATGGACGTTAAAAAATTAAAATCTTTATTGAAGTATTCATATTGTCCTTGATCTAAATTTAAGGTTGCTGCTATTGCAATTGATGCTGAGGGTAGAGAATGACCTGGGGTAAATGTTGAAAATGCTGCTTTTCCATCTGGCTTATGTGCAGAAAGATGTGCTCTTTTCGGATCAGTTGCTCATGGTGCAAAAGTTGGAACATTTAAAGAAATTCACATTATTTCTTCTGGAGATGATTTTATTTCTCCATGTGCAGGATGTCGTCAAGTAATGACAGAATTTATGCCCGATGATGGTTTAATTTACCAATACAATAAAAATGGCGACAAATGTAGAGTAAATAAAGTAATTGAAATGGTCCCATTTCCTATTAGAACAGAGCAAATTAAATAATGAAAGTATGTTTAGCTAGTATTATCGGCAGACCTAATGTAGGAAAATCATCATTAATAAATCAAATTGTTAAATATGATGTTGCAATTGTAACTAATGTTCCGCAAACAACAAGAGATCAAATTGTCGGCGTTTATAACGAAGATGATTTTCAATTAGTTTTTGTTGATACACCAGGTATCCACAAACCATTAAATTTATTAGGTGAAGCCTTAAATAAAAGTGCTTTTGAATCTACAAAAGATGTTGATTGTTTAATATTTCTTTCTCCTGTTAATGAAAAAGTTGGACAAGGAGATATTTTAATTTTAGATAAGATCAAAAATACCAAGAATAAAATTGCAGTCATTTCTAAAATTGATTTAGCCAAAAAACCAGAAGAAATACAAGAAAAAATTGAAGAATTAAGCAAATACAGTTTTGACAAAATATTGTCTGTTTCAAGAAATAATGAAAACTCAATTGAAGAATTAATTAAAGTTATTAAGCAATATGCCTATGAAGGTGAGCCATTTTATGATGAAGACTATATAACAGATAAATCAATGAGATTTTTAGCAAAAGAGATAATAAGAGAAAGTGCGATTAATCTTTTAAGAGAAGAGTTGCCACACTCAATTGCTGTAGAAGTTATTGATTTTATTGAAGAAGATGATATTAAAATAAATGCCATCATTTATGTCAAAAAAGCTTCTCAAAAAGGAATGGTAATTGGCAAAAATGGTCAAATGATAAAACAAATAGGAACCAACGCAAGAAAGAAAATGATGAGTCAATTTGATTCAAAAGTGGAATTAAATTTAAAAGTTAAAATAGCAAACAAATGAATTAATGATGGTAAAGCTCTTAAAAAATTTGGATATGAATAAATTGTTATAAAATTAACAAAAAAGGAGAAGTTATGAATCGTAAATATTTAGATAAATTATTTAAAGAAAAGAAAATTGATGCTTTAGTTTCTGAAGCGCCTCAAACAAGACTATGATATTCGAAAGTACAAACATCAGATGGATATATAGTAATTGAAAAAAATAAAGCTTATTTATTCGTTGATGGTAGATATATTGAATATGCAAGAAATAACGCAAAAAATGTAGAAGTTATTTTATTACAAGCTGGTACATTAAAAGAATTTTTTGACAAAAAAGCCTATAAAACAGTTGGTTTAGAAAAAGATTACTTAAATTACCAAGTATATGAAAATCTAAAAAATCTTATAAAACCTAAAAAAATCGAATGAGTTTTAGGACAAGAATTAAGAATTCTTAAATCAGCAGAAGAAAATGAAATTATGCAAAAAACTATTGATATTTCATTAGCTGCATATAAAGAATTAATGACATGAGTACAACCTGGAATGACAGAAAAAGAAGTAGCTGCATACTTAAATTATTTAATGAAAAAACATGGATCAGATAAAGAAAGTTTTGATGAAATAGTTGCTACAGGGCCTTCATCAGCTGAACCTCACCACCATCCAACAGATAGAAAATTAAAAGAAGGGGATCTTCTTAAAATCGACTTTGGTGCTCTTTACAAAGGATACAGTGCAGATATTACTAGAACATGTATTTTAGGTGGAGACAAAAAAGCGAATGATCCTAAACAATTAGAAATTTTACAAATAGTTATGGAAGCTGCAAAAGCTGGTCGTGATGCAGTAAGACCTGGAATTAAAGCAAGTGAAATTGATAAAATTTGTCGTGATTACATAACTAAAAAAGGATATGGTAAATACTTTGTTCACTCAACTGGTCATGGACTTGGAATTGATGTACATGAATTACCTAATGTTAGATCAACAAGTGACTATATTTTAGAACCTGGAATGATAATTACTGTTGAACCTGGTATTTATATTGAAGGTTTAGGCGGAGCAAGAAATGAAGATGATGTTCTTGTTACAGAAAAAGGTCGTTATGTATTCAGTAGACCTGAAGAAAAATCTTAGAATTTATTTTTAATAGCTTTAACATTTTGTTAAAGTTATTTTTTTATTGTGGAAATAATATAAAATTAGAAATATGAAATATGAATTAGGCCAAATAATAAAAGGAGTGGAAGCAATAAATTTTACTTATGAAGCATTAGGCTTAGTTAAAGCAGATGATTATTCTATTTTTGTTGAGAATTTATTGCCACAAGAAAAAGCAGATATACAAATTAAAAAATCGAACACAAAATTTGCTTTTGCTATAGTTGTAAAAAGATATAACGATAGTCCAAAAAGAATAAAAGTTTTAAATCAAAAATTAATGCTAAGTGGTTCAACTCCATTAGCAATTTTAAACTATGCAGATCAATTAGAATTTAAAGAAAATGTTGTTAAATATTTATTTCAAAGAAATATCCATTTTAATGAAACACAAAAAATTTTGCCGTGTGAAAAACAATGAAATTATAGAAACAAAATTACAGTTTTTGTTGAATCATCAAAAGGAAAAATCAAATTTGGTTTATATGAAAAAAATACACATAAATTAGTTGAGCAAGATTCTTATGATTTGGCGAATGAGTCTATAAATAAGCTTCTTATGTATTTAAACAAAAATATTAACAATTACAAATGTTTTATAGAAAACGGACAATTTTTAAAACAAATTGTTGTTAGATATTCAGAATCATATGATCAAATGATGTTAGCTTTTGTTCAAGACAAACCATTTGAATTATCTGAAGATTTATTAAAAGATTTAACCTCAAATTTTAAAAATTTAAAAGTTATATTAAAAGAAGAAAAAGTTAAAAAAACAACTTATACTTATTATTCAGAAGCTAAAAACATACAAGACAAAATTAGCAATTTAACTTTTAATATTGATTACAATAGTTTCTTTCAAGTTAACTCGTATCAAACCAATAATTTATATAACTTGCTAGTTAATAATTTGCACCTTGATAAAACTATGAATGTAGTTGATGCATATTCTGGAATCGGAACAATAAGTTTAAAAATTGCAGAAAAAGTTAAAAAAGTTTATGGACTTGAAATAGTAAAAGAAGCAGTTATAAATGCTAAGCAAAATGCAAAATTAAATAACATAAATAATACAGAATTTTATGAAGGCGATGTTTTAAAAACTATTGATAACATTAAAGAAAAAATAGACACAATAATAATAGATCCTCCAAGAGCAGGAGTATCGAAAGAAGTTTTAGAAAAAATATTAAAAATTGAACCAAAACAAATTGGTTACATAAGTTGTAATCCTCACACACTTTGCAGAGATATAGATTTATTAACATTATCAAAAAAATACAAATTAGAATTTTTAAAACCATGTGATATGTTTTGTCAAACACACCATGTAGAAACAGTTGCAATTTTAAAATTAAAATAAAAAAGAAAAGTTGTTTTAAGTGAGAGATATGGACTTAATTTTGACACACCAATAAATATGGAATTAGAATTTCTAGAATTTAATTATTTATTTAAGTCCCCTATTCAAATAGTAAATTATTATTCTATGAAAAAAATGATTAAAGTTGCAAAACTAAATTCAGAATTATTAAAACTTTTGGTATGCAATTAGATAGTGAAAGTATTAAATCTTGATTACAACCTATTAACAATTTCTTAGTTGTAGATTTTCCAAAAACATTTAATAAAGAAATCATTATTGATGAAATAATGGAAGACATTACTTATGATTTATATATTATTTATTCACAAATCTCTATGCTTGCAGCAACAAAAAACAAGACAATTTAATCAAAATAATCAAGCTTTATATATGATATAATTTTTATTATTTTAGTAAGGAGAAACTATGGGAGTTACAACTAAACCAAAGAGTTATTTAGATTTTGTGAATGAAAACTTACAATCATTTAAAGAAATAGTTTCAAACAGTTTTGATTCAGTTATTAAAAATAAAAAGAATATTTTGTCAAAATTAAAAATATTACTAATTGTTTCAATAGTACTATTAGTTTTAGGTGGAATTGGATTAATATTATCTTTCTTTTTCTTCATAAAAACTAATTCAAAAAATACAGCAATATTTGCTGGATTAATTATTTCTGCAATAGTAACAGGAATTGGTTCAATTGTATTATCAGTATTTTTAGGAAAAAGGAAAAAACTTTTTAAAGAACTCAAACACGTTCTTGATAAAGAAAAATTATTTGACAAAGCTTTTGAAAAATTATCATACAAAGCAATCGATCCAAATGACCCAGAACAAGCACAATTATTAGAAAAATTTAAACAAGGCTATGATAAAAATAGAGATTGTTTAGACTGAGTAGATCATATTCCTGGTGCATATTGAATTCCTTCAGATGCTAATGTAGACTCAACTGGAAAAATTTATTTTGTTATGGACAAAAATGAAAATCCATGAGTTATTCAAACTGTTCGTTATCATTGAGTAAGAGAAGAAAGAGACTTAAAAGGAAATATAACAATTAGACATATTTATGATTATGCAACATTATTTGAAGGAATCTTCTATTCTCTTCCTAAATCTAAAAACTTTACTTTAGCAATTGGGAATAGATGCGGATTAACTAATAATTTAAAACAAAATATCAAATATGAAAATCAAGAATTCGACAAATTATTCAAAGCTTATTGTTCAGATTCATTAAAAGGCCACAATATTTACCAACCTTATCCAATGGAGATTACACTTGAACATTGCAAACAAAATAAAAGTTATGTAAAATCGTTTGGTATGCTTTTAGATGATCAAAACATTAAATGTTGAGTACGTCCTCGAGGTTCAATTTTAGAAATTGATATGCCTTCAAACACAATTAATAAAGATAAAATTGTTAAAACTGTATGTAAAGATATTCTTGAAGATACATATTCAGTTTACTGATTATTCTCACTTCTAACAATACCTGGATATTTCAACTAATTTTTAATAAGATGAAGTAATATACTTCATTTTATTTTTTAGTAAAATTTATTTATAAAATTGGAAAGGAATTTATGGCTAAGATTTTTGTACCAAAGACTTTTGCGGAATTTAAAGATACAAATAAACAGTTTGTAAATGACAAAATACAATTAATTATAGACAATAAAAATAAAGAAATTAAGACAAGTTTTTTATATTTAATTACATCAATTGTTTTAATATTTCTAGGAATAATTTTTTGAATTTCAGGCTCACTTTGTTTATTGCTAATAAGTTCTTCTAAATTGTTTTACTGAACAGGATATTTACAAATAGCATTATTCTTAGCTTCAATTTTTGCAGGAACAATTTTATTAATTACATTTTTGAGAAAACAAAATTCGATTGTTACAAATATTGACAAAGGTCTAAATGAAGAAGATATTTACAAGCAAATTTTTAACCAAATTAACTATAAAATAGTTGAGTCGGAATATTTAGAAAATGTAAGTGAACAAGAAGAAAAGAATTTTTTATCAAGAGAATTAAAGAAAAAAAGTAATATAGAAGAAAGAAAAGAATTAACTAAAAAAATAAATGATATTTTAATTAATGAAATACCTTTTAACTGATTGAACAATTTTCCAGATGGTTCTTGAGTGTCTAAATCAGCATTTATTGAAGAAAAAACAGATTCAAAAGGAATAATAGATAATCAAAAAAATCATTGAAATATAACATCAGCTAAATTTTCATGGGTAAGTAATCCAATTGACAAAATGTCAAGTATAAGAGAAAAGGACAAAAGTCAATGAATAATATTTTTTGAAACTAAACTTAAAAAATTTAGCGAATCAAGTAACAAAAACTTTTCTTTCTCATTTGGAAAGAGAAGTGGAATAAACAAATTATTTAAAAACAATATCGGATTTGAAAATAAAAAATTTAATGATATTTTTAGTCCTTATTCAAATGACAAAATAAAATCCTTAAAATTTTATGGACCATATGCAATGGAAGTTTCGGTTAATCACTTTGAACAATATTTTGATAAAAACAAAGATTTTGGATTATTTTTAAAAAGAGATTTAATTAGAGGCTGAATAAAAAGGAATACAAAAATATTAGAATTAAATTTACCAAAATACATAATCAAAAAGCAAAATTTTATTAACAAAGTTGTAAACAATTTGTTAGATCAAATATATTTAGTTTATTGATTTATATCAATGGTTCAAATTTCCAATTATTCATAAAGGAGAAAAATGAAAGAAACATTTAATCAATTTTTATCTCGTTCATATGTTCCTTTGTCTAAACATTTAAATGAACAATTAAATAAAGAAGATTCAGAAAACAAAAAGCTTAGTAAATCTTTCTTATGAAAAATAATAATGGGGTTGTGTTTATTAATTATATTGACTTCAATTTCAATAGTTCTGATGTTGTCATTTTTTGAAAAGTCATATAAGATTGAAATTTTAAAATGGGCTACTGTTGCAATTTTGATTATAACAAATTTGTTTTGCGGAATAAGTTTTACATTTTCAAGAAAGGCAAAAGTTATTGAAAAAAGTCGAAATTTGGCAAAAATTAATCTTAATAATTCATTAAATTATTTATTTAAATTACTTAATTTTGAATACAAAAATGAAGAAAAAAATAACAATATTCCATGAATTAATTATTGCAAAAATCTAACTACAAATGGCGAAAATTCTAAAGTTAAAATTTTAAGAAAAGCACTATTAAACAGTGAAATTGGTCAAAATAATACTTGAAAAATTCAAGAAGTAACATTTGAATCAAATGATAAGAACGAAACATTTTTATTAATTGAAGGAAGTCATAAAAATATGACTGAAAATTCATATATTTCATGTGGAAGTAAAAATGAAATTTATGATTATTTAAAAAAGAAAAATAAAGTTAAATTTATTCATCAAAATGATCAAATTCATTTCAAAACTTCAAGTAAAGAAATATATGAACTAACTGACACAATTAAGACTTTTGAAGAATTTAAATTGAATGAAAAAGATTATGATTTTTAATTGATCCAAAAAATAAAGTGTCTTGCAGTTGAATAAAAATTGAAAATAACTTTTTAAATATTCAAAATAGTTCTGATCCAGTAAAAACAATATTAAATCATCTTTATTTGATTTATGTTTTAACTGAAATAGTAAAACAATTTGCAAAATAAGAAGGTATTGCCTTCTTATGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGATTGTCATGAAGAAATGCAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAATTGTGAACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTATAGGCAAGAGTAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTGGTGCTGTAAACTAATTGTGGGAATGAATTTAAATGAGAGAACAAGCATAAAGCTTGTTTTTTTCTTCTTTCTATCAAACTAAATATAGAAAGGAGGTGAAATTATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGAAAGAGTAAAAAGTATTTAAATTTTGCTGATAAGGCATTGATTTTTTTATCTACTCTATTCAAAATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGATATACAAATTTTACTTTTGTAAAATTAACTTATAAAAATTGAAAAACTAAAAAAGAACAATATTATTACTTAAAAAATGAAGAGGTTTGAAGATTATCTTTAATCTCATCATTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAACTTATTACAAAAATGACTTTGGAATGTAAGGCAGTTGTTTATCAAAATGACTGAAAATTATATAAAAAGTAGGTTATACAAAAATTCACTGATATTTTAAATATCTGTTTTTTAATGTTGCATTTTGAAAAAAACACTTGACATTATTTAACTTTTGATATTTGTCTATTCAAAATTGAAGGAATAAGTTATTAAGACTTTGGAATGTAATGGAAAAGTCTTAATATATGAATCATTGATTCATAAAGTAATCTATTTAACTATGAAAAAGTTTAAATTATCACAAATTACGAGAATTTATTTAAAAATTTGCAAAAAATCTAACAAATCTTGATCAGAAATTAAGAATTTTAAGAATATTTATGATTTTGTGTTTGCTAAATCATCAGAAAAATATTTTTTTATCAATTTAAAACATATAAATGATTCAAAATTTAAGCAAGATTTATGGAATTTTTCGAATTCTTACAGAGTTTATGCTTGTTATCAGATTTATGAAGAAATAAAAAGCATCAGGAGACCTCTTGAACAAGTATAAATCAATTTCTCTCTGCTCTCTCATTAGCCAAGCAGCTCGGCAAGGTAATCAAAAATGGACCGAGCAAGCAAGCATGGAGCAAAATCCCAAACTAAAGTGGAATTTTTAAGCTAAATTGTCAGAGAACTACCTTCAAAAACTCTCTAAAAAATAACCTAAAAAACCACTTGATAATCAAAATGGTGCTTGTTGTTTAGTTTTAGTAGCAATAACAGGTACTATGATGGGAACCTATACAGCAACGCCGATAGTTTATGCTAGTTATCAGATTTATAAAAAATAAAACAATAGGAGAAAAATATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGATTTTTAGATAATGATTATATAAAGAATATTAGATTATTTAATAATTTTTATTATGAACAAGTAGACTTAAGACCTTATTATGGAGCTGATTTCTTTGAAATATTAGATAAAAAAGAAGATTGATTAAAGATAGAAAGTTTTATAAAAAAATTTAAAGAAAACAATCAAAATTATTTTTCGAAAATGCCAGATTTAGATCAATCAGATGAAGCACTTAGAGCATTGCTTTATTTGGAAAAGTCAGAATGAACCAAATTAAATTCAAATAAAGAAATTTATAGAAATATTAAAGAAAATATTTTCAATTTAAATGATAAAAATTCTAATTTATTCAAAGCAATTGCAAAAAGGAACAAATCTTATTAATTATGAAAGAATTAATAATTTATTTAAGTTGAAAATATGAAGGAAATATTTGAAAAATTTATGAATTTTTAAAAACTTACAAAACAGTTGCTCAAGCTAAAATAGATGAAGTATTAAATGAACTAAAACAAAAAGGCATTAAGTATATAACAGCTTTTGATGATAATTTTCCAGAAATATTAAAAAATTATCGCTATTCACCATATGTTCTTTTTTATAAAGGCAATATTAAGATTTTGAAGTTACTGGAAACTCAAAACAAAAATGAAGTTTTCATAGAATGAGATTTTGAAAATAAAAAAATTTTATTCACATCATTAAATAGTAAAGGTAAATCTAAAATCATTTACATACTAGATTGTGGATTTAATCATCTTAACAAAAAAATTAATTATAAAAAAGAGTTGTATATCACACAATTTCCATTTCAGACATTACCTAACAAAAATAATAAAGAATCAAGTGAAAAATTATTAAATTGTTTATTCGATGCATAAGTGCAAAAGGAGGAAATATGTCAAATTTAAGTGAAAAATTAAAAAATTGTAGTGAGCAAGAATTAAAAGATTTCAATTATTTTATAGATGGTATGTTAGAAATGCCATTAATAAATGCTATTGATGAAGTAATTGATTGTCTTGAAAAACCTGATTATTGTGAACATTATGATACTCATTGACAATTTTTAAAACAATCATATATTTTCATAACATATAGAATTGAAGATGATATAAAAGAAACTAAAGAAGTAAAGACATTATTTAAAAAAAATTCTATATTAATTGATTTGATAAAACCAATTGAATTTTGATTAAAAATAATTAAATTGTCTGTTAATTTTTATAAATGCAATGAATGAGATATAAAAGAAACATATATAAGAAAACCAACAATAGATTTTTATCATTACTCGAATTCTTTACATGATGCAATCTATGATGAATTAGAAGCAAGAAAAAAGAAGAATGATTAAATGCGTATTTTGCAAAAAGTTATTGTTATCAGAAAAACCAATTAAATCAGTGTCTAATAAACCAAAAAGACTATAAAACAGTCATTTTAGTAAAATATATAATTTAACACCAATAAATTCTGAAGTTCAAAAATTAATTAATAAATCAAGTGAAAATATTAAATTGTTTATTCACTGCATAAGTACAAAGGAGAAAATATGACTAGAAAAGAATACAAAGAAATTATTAATATGAAATCTAGAACTTTAAAAATGGAATTAGTAATAGATTGATTACTAAAAGTTGGCATCTTCAGTAGAAGAGAAGCTTATTTACTAGATATAAAAAATTTTGAAGTTGAAAAATTAATTAATGAATCTATTAAAAGAAAAACAAATTATAAAAACTGACAAGAATTATTTAAAAAAGAGCTTGTTAGATAAATATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAATATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAAAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAGAAAGGAAAAAAATATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGATCTTTATATGGTTTTAAAGAATTAGAAGAAAGAAAATTATTTGCTGATTTACTTAATTTAAATGGCATTGGTCCTAGAGTAGCATTTAATGTTTTAAATGTTGGATTTGATAAAGCTATAAATTTAATTAGAGATGGCAACATTGAAGAGATTGCAAAAATTGAATATATTAATCCAAGAATTGCAAGAATAATTGTTGAAGAATTAAAAGAAAAATTATCATCAAAATCAAAAGACGTTAAAAAGTTAAAACTATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAAAACAGTCATTTTAGTAAAATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGAGAAGGCTTTAAAATATTCAATTTAAAAAAGTATTCTTTAAATAATCTTAATCAAATTATTAATTACAATAGGCTAAAAACTAATTTTAAATTAGGCAATAGTCTTGTTGAATATATTCTCAATAATTGTAATAATAATCCTGGAATATTAAATGAAGTGTATTTGAAAGCTATTGATTATTTTGCAATAAAGAATAAAGAAATGAATTTAAAACAATTAAAAAAATTATTAAATAAAACAAACAAAAAATATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGAGAAATTTTTCTATCTAAAAATTATGAGTCCATTTTTAAATATTGAAATTCATTAAATAGTTTAAATTTTTTAAAAAATAATAGTGTTGATTTAGTTATTACTTCACCACCCTATTTATCTTTAGTCGATTACACAAAAAGCAATTGATTAAGACTTTGATTATTAGGATTTGAAAAAAATAATTTAAAAAAAGAAATCAAACTTTCAGATTCGCTTGACTTAAAAGAATACACAAATTTTATAAAAAAATACTTAATAAATATTTCATCTAAATTAAAACCAAAAGCTAAAGTTTGTTTAGTAATTGGTGATGTTTATGATTTTGAATTAATAGAAAACATTTGAAAAGAAATTAAAAATGAAGTTCCTTATTCATTCCTAGAAGTAGGAATTGATACCAATACAAATAATTTTAAAACTTCTAAATTACTTAATTCTCGTAAGGGCAAAGCAACTAAAGTTGAAAAAATTCTAATATTAGAAAAACAAAATTAATAACTATAAAAAACAAGGAGAAAATATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGATTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAAAAGAAGGAGTTAATAAAAAATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGAATCAGAAAAAGTGATGGTAAATATCCTAAGAAAAGATATGAAATAGCTTATAGCTTATCAAAGCAATTAGAACTTATTAAATACTGTTTTGATGAAAAAATAATATTAAAAGGTGCTGTCGTTTTCATAAATGATTTTGAAAATAAAAATCCAGATAACTTTTTAATTCGAAAATATGATTCAGACAATGGAATGGACAAAAATTGAGTAAGTTGCTATTATGATAAAGATTTTTTTGAAAAATATAATTCTTTAAAATCTTGAAATGATTTAATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGAAAAGAAAATTCTGACAAAAGAAGAAAGGAAAAAAATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGAATACTAAAATATTGTGGTGAATTAAATTCTAATAAAAAATTTATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAAATATTATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGATTAGTTAATTTTTGATTCCTATTTACTTTAATTCTTTTTTGTATCATATTATTACCTATTTCTTTAGTGCTAGCAATTAATTCAGAAGATGTTTTACTATTAAAAATGTCTTCTAAAGCATTGCTGTGAACTTATACTATCATTAATGTTGTAATTACTGTTTTTGTTTTTTATAGATTTGTTTCTTTATGAATTATTGCATCTAAAATGCATAAGAAACATACATTAGATATACATAACAAAAAACTAGGTATAAAAGCAACATTATGACCATTTATTAAATATAACAAATTAATTAGCTAATTTACTAAAAACACTGTTTTATAGTATTTTTAGATTATCTTTAATTAAAGATTTATTTTAATATAAATATAAAGTATAATATTTATATAGCAAATAAGGAGCAATATGAAAACTAAAAAGATATGATTAAAGACAATATTACCAACAATAACATTACCAATTGTAACTGCTTTAACAGCAACTAGTTGTAATTGGTTTGCTTTTCCTATTAATCCACCAAAACCATCTAAAAACCCAACTATAAAAAAGGAAAATGCAGAAGGTTTTAGTGATAAAAAAGTTATTTCTGCCAAAAAATCTATAAGACCAGAAATTAAAGATTTTAAAAATATTGAGTTTTATATGGGAGATAAAAACTTAGCTAATCATGACGGAACACTATGTGCAAATGTTAAGAATTGATTAGATTTTCAAGTTCCTGGTTTCTTCTACTATGGAATATATTTAAAAACATTAATTAGTGGAAAATATTTAAAAGAAAACTTAAATGAAACAGACCTTTTATCTATTAACACTAGAAAAGAAAGATTAATTAATGAATTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGAAAGAAAGAAGATCTTTTTTTAAGTGATATTAACTTTTGCATTGAAACTTCTTTTAGTAAGAAAACTTGACCTAACTTAACAAAAGATGAATTCATAAATTTCTTTAAAGATAATGCAGTTAGATTTTATGATAGATTAAACAATAAATATGGTTACTCAATTAACTATGAATCAACTCATGGAAAATTGTCATCATTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGAAATTGAAAAGCATATACTAATATAGCTTTTCAACATAAAGCAGACAAACTTTTTGGTTCTAAAGAATTATCTAAAGATGCTCCTTTATATTGATTATTTGAAGCTCAAAATGAAGAATGAGATCATTCACAAGCAAAATTTATTGCTAATGAAGTTATGTATTATTTAAAAGAATTTCTTTTACCTTTAAGTAAATTATTAGAAGTAAACTATGATTTTGAATCTGTCATTTGAAAACAAATAGATTTCTTATATGACGTCGCTAAATTAGATGCTAGAACATTAACAAAATTTGAAACAAAAAATAAACCTGTTAATTATGCATCAGCAACTGATGAAGAATTAACAAATAAATTTAAACAACTCCAAGATACTTATAAAATTTTTGGAGATATCTCAAGCTCAGACTTGAAAATAAAATAAAAAATACAAAATAAATATTTAGTTTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAATTATGTTTAAAGACCTTTTTTGATGGCTTTTATTTAAATTATATCAAGCCCCTTGATTTATATTAATTGGTTTACCCTTATTTATTATTAATAGTTTATCAAAATTAATTAATTTTGTTTCTTTTGATTTTCTTAATTTAATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGAGTATTTACATTTTTTGCAAAATATGGCATTAATGTATTAAACACTGTAATTAGTGGTGGTCAAGAAACAAATGAACTTGGTAATTTAATTTATCAATCAGTTGCTCCTGAGCCTAGATTTATCGATCCATTTTCGATTACTTTTGAAGAATTTATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGAAAACAACAATTCTTTTCAAAAATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGAAAAAAAGAATTAGCAAGTACACAAAAACAAATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAAAAATGAATCAAAACAAAAAAATATCATCATTAAAATTAAATATTTGAAAAAATTTAAATATTTATGATGCAATAATTATTCTTGGAATTTCAGGTTTTTCAGCAGCTTTAGGCTTTGCTAATGGATTGCCTTTGTGAATAAAAATCATTATATTTTTAGTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAACCTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTCAAAATGATTTTGAAAGTCTTAGAAATATTGAAAACATAGATGTCTATTATTTAGTTATCTATGCTAATGAAATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGAGCTTGTGATTTATTTAACAGTGAATCTTTAGTTATTTGAAACTTAAAACCTATTAATGAATCCACTAAACAAAAAATACTTGATAAAGCTATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGACCTTGAATTTCTAATTCTTTAAATGATGGTAATAACTTTTTATTAGGTTTTACAACAACCTCAAGTAGTCCTGTGATTTTTGACTTATTTAAAAAGACAAAAGACAGAACATCATTTTCAATGATAATTTGAGGTGTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGAATAGAAATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGAGAAAAGAATTTTTTAACATCTGCACCTATAGGAAATGGAATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAAGCAATAATACTATAAAACAGTGTTTTTGGAGAAAGGATAGTATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGAAATGAAAGTATTAAAGATAAATGCCCTATAAAAAATGCTAACTGAGAAGAAATATTTGATATTTTAAAATCTAAATTACCATACAAAAAAGATATTGAATTGGTATCTTATTCAGGTATGAAAGATAATTTAAATTCAAAATTAAAACTAAATAAATTCAGTGGTGAAAAAACTCCTAAAGAAGAATTACTTCAATATTGAAAAAATCTTGACATTAATTTAGCACTAAATAATTATTTAAAAAACAAAAGCATTAATAAAAATGAATTTTTTAAATTTGATTTTGAAATTGTAGAAAATCAATATTTAAATATTTATTTAATTAATGAAAATGTTAATCTTAATAAAACAAGTAGATATTTATTGATTAAAAATCAAAAAATAAAGTGAAAACTAAGTTCTGATATTTCTCAGTTAAATTGAAAAGAAAGATTAATAATAAATTATGGATATAGTTACAAAATAAATAATGATGGAACAATACAAATAAAGCAATCTAAATCAAAAAATATAACTTCAAATGAAATTATTTGTGAATATCCACCAACAGAAATTTATTTTGTTTCACCACAAAATTTACAGGAAAAATTTGAAATTAGAATTGAAAGTGAAAATGGCGAAAAAATTGTTACTTTAAACAATAATGAAATTTTTTATAGTGCTATTGATATAAGGAAAATTGTTAAAAATAAATTAAATTTTTCTAATAAGCTTTTCTTAATACATGAAGATTCAAATGGCATTAAAGAAAAAATTCAAATCAAATTTGAAAAAAGTGATATATCAAACACAATTGATTTAAAATATCCGAATTGAAATCCTGATTTATTAGAATACAAACAATTTATAAGCAAAAATGAATTAAATAAAGATGGCAATGAAATTAAAGATGCTAATGGGAATCCTGTACCATCAAAAAAATATGACCCTGGGATCGATCCTAAAACAGGACTTAAATCAGAAATAGTTTGATTAAATTTTAATGAATTTCATGATTTTTTGAAAGAAAACATAAAAAACTATTGTTCTGTCTTAATAACAGAAAAAGAATCTAAATTTCTTTACGAATTCATGAATGATAAAAAATTCCGCTGAGTTGAGTTTTTAATAAATTATGAAATTTTAAAAGAAGAATTTGATCTTTTTGATAGATGAAATAATGAGGGCATTGGCCCATATCCTAATGAGGGGCGCTACATTGGAAAAAATATTCCTTATGATGAACAAGAAAAAAATATCAAAATTCTATATGAACTACGAGATTTATATGCCAGAATACAAGATTCTGGCATTGATAAATCTGAATCAATAAACATAGATAGATATTTCGAATTAAAACAAATCGTAAAAAAATATAAAAATCATTTTGATTCAACTAATTGTAGATTGCCTTTTGGTTCATTTTATTTAAAATATAAAAGTAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTGACTATTAATGCAAATTCAAATATTTTAATAAGAGAATTAAAACAAAACTCAATAGGAAAATGAGAATTTGTAAATGATTTAGAAAATATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGAATAGATAATAATAGTACTACATATACAAAACCTAGTTATTTAACAGAAGAAGCAAATTCTCCATCATTTATCAAAAAATTTTGAAGAAATAAAAATCTTAAAGTTTCAAATGATTTTGAAAAATACTTAGCCAAGAAAAAAATTATTGATTTAAACGCAACAAAAAAAGATCTTGACAAATTAAAATATGATGATATTTTTAATGAGTATAATAATTTTTTAATTGAAAAATCTTTTGAAGAAAATTTTGAAATCAAACCAGAAATTAACATTAACAAATTAAGACAAGATTTTAAAACAAATGAACAATTTTTAAATTATATAAATCAAAAGGATAATGAAGCTAAATTTATTGAAAATTATGTTATCTCAAAAAATAAAGAATTTTTAACAATTAAAAACTGTTATTTTGATTCTAAAAGTAACTTAATTAAAGTTGTTATTGATTTAAAAGAAAATGCTAGCTTAAATAGTTTTTTTATTAGAAGAAAAATCTTTAATATCAATTTAGAATTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGAAATAAAGATGAAAATATTGCAAAATTTACAATAAACTTCAATAATAACTTTAAAACTTATCATCCTTGCACACTCCATGTTATCAACAATGCTCTAGATGACGATTTTAATTGACCAGAAGCAGGCTTAACTGTAAAAGTGAATGTTGATATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGAGATTCTTTCTTTAGTAAAATTGGCAATAATGAATTAAAAATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAGAAAAAATTACTTTCATGACTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGATATAGAAAGAAGAAGAAACGTTTATAGATTAAGCCTATAGGCTTCTTTTTCTATTTCTGTGATTTTACTAAAAATACTATAAAACAGGCTTATAATAAAAATATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGAATTCAAACACTAGCAAAACAACTAAAAGGCAATTTTATTTTGTGTAAATTTCATTTTTTAAGAAAATTTAATGATTTTATTTTCAAAAAGAGAAATCGTGGATTTAACAATTTTATAGATAGCATTCAAAATTTAAGTGGGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAGTATTTATATTAATATTAATATAGAAAGGCAATTAAATTTGCTATTTTTTCCACGCAATTTTCTTTGTAATTTACATTTTTTGATATAAAATATAGTTGATAGAAAAAGGAAATGCCGTTTTCTCTTTTAAATTTCTAACTTATCCCACTAATAATTTACATCACCAAATTCCTAATAAAAATCCATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGATAAGAAGGTATTGCCTTCTTATTTAATTATTATTTGAGATAAACAAAATATTTACTTTTCCATATTGTTTTTGTTTTTGTATTGTAAGCTTTTTTGGAATATCAATCATTAAGAAATTGTCAGTTTCTAAAATAATTAAACCATTTTTAGTTAAAAATTTATTATCAACTATTAATTCAAGAGATTTATTAATCAATTGATATTCCTTGTATGGAGCATCAATAAAAATAAAATCAAATTTACGTCCCGAATTCTTTTGTAAATAATTCAAAACATCATCTTTTACAATATCGATATTTTCAGCTTTTAGTTTTAAAGCATTATTTCTTATTGCTTTAATTGCTTCATTATTATTATCAACAGCTATTGCTTTTACCGCATAGTTGCTTAATGCTTCAAAACTAAAAGCACCACTACCTGCAAAAAGATCTAAAATGCAAGCACCTTCTAAGTTAAATCTTATTGATGAAAAAATAGCTTCACGAACACGATCCATAGTTGGTCTTGTTTCCAAAGTTTTAGGTTGTTCTATATTTAAATGACGATATTTTCCACTGATAATTCTTAACATTTTATTCTCCAAAATTAAAATTGTTACTTACATTATATTTAGTATTTAATACTTAATATATAATATTATTATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTATATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAACCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAATTAATGAGGTATTTATGAGAACTTATGAAGATAATTGAAAGGCATTAGCAACTAATGGATGATGCGTCTTAATTTCCATATCTGTCTTTTTGATAGTCCTTTTGGTCGCAATTGCTATTGGGGCTAAAAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTATGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGAAGTGATGAAGAAATCGAAAGATTGCCTAAAGTTATTGACTGATTAAATTATTGTTTCAACGATGAAGCTTCTTTTGATTTACTTAACAATAGTTACAATTATCCAACAGGCGAAACTATTAAATACTACTTTAAAGGTGTATATAGTAATGATCCTTCAAAAGCAACATGAAATACCAAAGTATTAAAATATGGCAATGTTTATTATTACAAAAAGATGAATTTTGGTGGACCAGATAGTTGGTCTCCTGCAACAACAACATATGCAGAATCACTAGAAAACAAATTTGATTTTAAAACAATGTTCTCATGAAATATTTTTAAATGATCTCAAACTTTAAGAACATTATCGAAAATAGATTTAGATTCTAAATTTGAAGTTAGTGACAGCAAAGTAGAAGACTTTACAAAAATCAAAAAAATAAAAAATAAAGATATTCAATTCAAATTAAATGTTGAAAATGAAGTTGTTAGAAATAAAATTAAATCGATTTTTTACAAATTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTCAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAATTTGGTAAATTACTTGCAAAAAGTTGCAAGTTTTTTATTTTTCATACAAAAATTGATGACTTTAATTGTTTAAATTTTTAAAAAATTTATACAAACGTAAAAATTTAAATTTATATTTAGTATTAACATTAAAAATAGTATTATTAATATATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGAGAAATTGTCGATAACTCAATCGATGAGGCTTTAGCTGGTTATGCAAATGAAATTAGTGTAACTTTAACTAAAAATGGATCAGTCAAAGTCATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATTGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGACCAAACTATGAAATTTTTAAACGCGCAAAATTTAGTTATGAAACTATTGCTGAACGTCTTAGAGAAAGTTGTTTTTTAATTGGTGGATTAAGAATTCACTTCAAAGATGAAATAAGTGATCGTGAAACAGAATTTATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGAATTAATGAAAATAAACCATTAGCCAAAAAAATACTTGAAAAAATTAAGAGAGCTTCAGAATCTAGAGCCGCCGCAAGAAAAGCGAGAGCAGAAGCTAGAAGTACAAAAAATGCTTTAAAAGAAAAACAAATTTTAAGTGGTAAATTAACTCCAGCTCAAAGCAAAGTGCCTGCTGAAAAAGAATTATTCTTAGTCGAAGGTGACTCTGCTGGAGGTAGTGCAAAACTTGGACGTGATCGTAAATATCAAGCAATTTTACCTTTAAGAGGAAAAGTTATTAATACTGAAAAAGCTCGTCTTTTTGAGATTTTAAAAAATGAAGAAATTGGAACAATAATTAATACAATCGGCGCTGGTGTTGGTGACGAATTTGACCTTAAAAAAGCTCAATACGGAAAAGTTGTTATTATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGAGATGAAAATGAATTAAGAGAACTTTTACAGTTACCTAACTTTAAAAATGCTGAAGTACAACGTTATAAAGGACTTGGTGAAATGAATGCTGATCAATTGTGAGAAACCACAATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGAATTAACAAGAATGTTGAATTTAGTTTAGAAGATGATTTTGAAATATAGAAAGGAGCCATTATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGAAATAAACCTTTTAAAAAATCTGCTCGTGTTGTTGGTGATGTTATTGGTAAATATCACCCACATGGTGATAGTTCAATTTATGAAGCTATGGTTCGTATGGCTCAAGATTGAAAAATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAACAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGAAAAGATTTTGGAGTTCACTTAAGTGACTTTGTTGAATTAGTTTCAGGTGAAGAATTAGTTGGCGCAATTAATGTCACTGACTTTAACATACAAAACTATGTTTGCTTATTTACTAAAGAAGCTCAAGGAAAAAGAGTTGTTTTAAAAGAATTTGAAGTTAGTCGTCCAAACAAAACTTTTACAGCTATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAATACTATAAAACAGTAGTTTTAGAAAAATTGAATAATTTTAAAATGGTTCTTTATGAATCATTTTTTGATTTTATTTTATAATTTTAAAAGAGAAAATTATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGATCATTAAGAACACCAAATGTGTTATTTGATGAAACAAAAAATATAGATTGTGACTTTTTAATTTGTGGTCACTCTCATTTTCCTATTTATTATAAAGATGTTAAAACTCATAAAATTTTGTTAAACCCTGGCTCACCTTCATTGCCACGTTGAAATTCAAAACCAAGTTATGCAATAATTACTATCAAAAATGAAAAAAAATATGAAAATGTGGAAGTTATTTTCAAAGAAGTTAACGAAAAAAGTTAGTCTTTGGTAAAATTTGGGTGATATTTATTATATTTTACTTTTCTTACAATTATTACTAACGAAAGGAACAAATGATTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGAAGAAGTACAAAACCAATTTATAAAGCTTTAGGACTTGATACGGTTGTAGCTAACTACAATGGTGCACAAATTCACCACCCTGAAGATGAAAACTTTATTCCTTACATCAAATATTTAGACTTAAACGAAATGCTCTATATTTTAGGAGACCCTATAGTTAAAAATGAAATTAGCAATGTTGCTATTGAGGGACCTGGTTGAGTTCAACTTCAACATAGAGATCCTGATTTAGAAAGAGTTTTTGGATTCTCAAGCAGTGCTAAATTTATCGTTGGTTTAAATTTTGAAAAAATTCCTCTTAAACCAACTGGAATTATTTTTGATGTTAAAGAAACCACAGATGTTGAAGCATTAAGAAAATACTTAAGAGCTAAATATGGTGACTTAGGTGAATTCTCATACTGATCAAAAGGTGAAGGTTTAACTCCAGTTTTTGATATTACTAATGTTTCGGTTAATAAAGGTAGAGCACTTTCATTATTAGCTAGATACTATGGAATTGATTTAGAACACACTGTAGCATTTGGTGATGGTTTCAACGATGTGCCTATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTAGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAATTATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGAGCTGACTTTAATCAAATTCAAGGTGATATTTTACTTGCTTATAAAGCAGAAAGTATTAGCCTTGAAGTAGCTTGTGCATCTATTATTGCAAGAGATTATTTTTTATACTTTATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAATTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAATTAAATTCAACTTTTCTAAAGCAAAAAATAAAAAAATATTATCATTTTTATGCAAATTAGAGACGTGCGGATCTAATTATTTTTTTATTCTTTTTTATTGATTATATATTAATCGAAATTTAATTTTTCTTTTTAGTAAGCAATATTTATTGAGTAATTATTGAGTTAATTTGTTAAAAAAAATTGAAAAATTTAGCACTTTTATGTTGTGAGTGCTAAAAATGTGTTATTATTTATACATATACTAAAACGTATTTGAAAGGAAAATTATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAACTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAAAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGACGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGAGACCATGAAATTGTAGATTGATTAGTTGCTAAAATTAAAAACGATCACAAAATTGACATTCGTGAAAACAAAATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGCGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAGTTAATTTAAAAATAAAAAAGTGCCAAATGCTGGTACTTTTTTATTTTTCAATAACTAAAATAAAGCTTTTATTGAATAAAAGTATACTTTTAATATATAAAATAACCAAAAAAATGAACAAAATTAATTTTTTTAATTTTTTTATGCTTTTTTGCTAGCATTTTACTCTTTTAGAATAAAATAAGAATATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATCAGCATTTCATAAATCATTAAGAGGAAGTGATGTTGATGCAGCACTTTATTATGGAGCTTTAATCTTAAAAACAGGCGACTACATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAACATGAATGATATTAAATGAGAAGAAATCAAAACATTTGAAGATTTAAAATTAGCTAAAAGCAAAGTATATTCAAAGGACTCTGAAATAGTTCAATTACAAACAAAAATTAGAACAGCTTCGCCTGAAGAGAAAAAAGAAATAGGTCAAAAAATTGCAACTTTAAGAAACTTTTATGAAGAAAAATTCAAAGAAATTGAATTTAAAATTGAAGCCGATAAAATCAAAAAAATAATTGAAAGCCAATATGTTGATGTAACTAAACCAGTTGACAATCCTGGTTCATTGCACCCAATTACAATAGTAGAAAATAGACTTAGAGATTGATTTATTCAAAACGGATATTTTGAATCAAATGAAAGTGAAATTGTTAGCGATGTTTATAATTTTCAAAAATTAAACATTCCTGAAGATCACCCTGCTCGTGCTATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGCAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCAACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGAACACTAAAATCACTTCTTTCTTATGTATTTGAAGAAGAAGTTGAACTAAGACTTAGACCAAGTTATTTCCCTTTTACTGAACCAAGTGTTGAAGTTGATGTTTATTATAAAAATGGTTGAATCGAAGTTTTAGGTGCTGGTATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATACAACATCAAGGATGTTAGAGAATTCTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAGCTTCCAAACATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCTTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGAGCAAAACAAGGAGTTTTATTAATTAATACTTGTTTAAGTGTTAATGAAAATCAACCATTAAGCCATAAGAATTTTGGCTGAGAAATATTTGTTATTAATTTAATTAAATACATTTTGAAAAAAAATAAAAATGTCATATTTGGCATCTGAGGAAATGATGCAAAAAGAATATTGCAAATTGTTCAAAATGACTATAAAATAGATGAAAACAAAATTATTTTTACTTCTCACCCTTCCCCTCTTGCATATTCAAAAACAACTAATAGTTTTAAAGATTCAAAGTTTTTTATAAGAGTAAATTCATTATTAAAAAATCCAATTAATTGAGATTTAAGAAAGGATTAATATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATTAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAGAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGCGACTTTGTATTTCAAGATAAAGAAATGGCAGGCGTTGTTTCTCAAGGTATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGATTTGATTACAAGAAAAACTACAAAACTGGAATTCACAAACAAATTCAAAAAGTTGATAATGGTGAAGCGAATGCTTTAAGTTTTCTAGAATGCAGATTCAAACACAAAGACACTAAAATCGAAGATATGTTATTTTTAGCAAAACATAATGTAGAAGCTAAAAATGTTTGAGCAATAGATGTTACAAATTTAAACTTAATTTATACTGGTGCTCCAACTCATGTTTATGATCGCAGCGAAATAGGCGAAGAATTAACTTGCAAAAAATATAGTGGCAAAGTTACTATTTTAGGTAATAAAGAAATTGAAGTAAAAAATGCTTTAGCTATTTATTCAAAAGATAAAGTAGTTTCATTAGCTTCAGTTATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGATTAGGAAAAGTAAACCCTTCATATAATAGTGTAGATGCTTGAGTTTGTGAAGTACATAAAGAAGTAAATAAAGGCTATAAATTATTTGAAAATTATGATTCAACACCATTTAAATCAATTGACTTAACTTTCGAATTAAAACCACATCAATCAATTGATGATAAAATTATTGAAATCAAAAAAGTAGCTTTTATTTTTGAAATTTATCAAATTGATGAATTTGCAAAACCAGAATCAAGAAATGTTACTTTAAGAATTATTGGAAGCAATGAAGAAATTCAAAAAATCAATAATGAATTCAATAATTAGGAGGCATTATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCGGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAACCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGAGCAAAATTTAGAGAAATTGCAGATGCATGCGGTGCTTACTTGATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTATGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGAGTATTTCCTGGATATCAAGGTGGGCCTCTATTCCATACAATAGCAGGTAAAGCTGTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGAAGAGAGCTAGCTGATATTATTGATACTATCTTAAAGAATCATGAAACTATTCTAAAACCAGAAAGTGAAAAGTTATTTAATAGCCTTAAAAAGAAGGTTAGAGATTTCACTAAAAAATTCCCAATCATTACTGTTTATAGATAAAGACGTCAAAAATGCATCTTTATCTTTTTATAAAAAAACTTGCTAAATAGCAAGTTTAATTTTGCATTTGAAGAACAGGCTGATCTTTATTACTATCATCCTCTTGTTCAAGTTGTTTTTTATATTCTTCTTCAAGTTTTGCGGCTTCTGCAAAGAATTTGTGTTCAAGTTTTCTACTTCAAGTAAGTTTAGCTTGATCAATTTTAGGTATAACTTTAGAGACATAAATGTAGCTTCAAATTAATAAAATTATTGAAGCGACTAAAGCATTAGCAATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGAACATTAAGCGCAATTAATCATAAGTTATCAGCTGTAGCAGCTACAAAACTGTAAGCCAAAATTGAATAGATAACTGCAGCTAACATAGTTCAAAGATAAACTTCTTTGCATCTTTTAAAGTTTCTTCGCGTATAGTTATATGACATTAAGCTTCTAGCTCCATCATTAATACCAAAAATAGAAACTAAGAATAATGAAACAATCGGCATACCTTTTGACCAATAATATTGGAATGAAAGTGGTTCCGCAAAAGGAGTTTTGGTTAAGAAAATGTTCATAACAGCAAATGAAATAGCAACACCAAATGATCTTATGAAACCACTAAAACCTAAGGCAAAAGTAGGCAACAATAATTTTTTATTAAATTTAAATTTGAATAAGTGTCCAAGCGAAAGTCAGGTTTTATTCCGCTTGCTATTAATTACCACATAAGCTAAGTAGGCAGTTAAGTTAATTGACCAACCAATAATAGTAGCAACAACACCACCTAATACACCCATTTTACATGGCACAATAAATACTCAGTCTAAAACAACATTTACAATGTTTGCTCCAATAACTCAGCCCATAACATAAGCGTTATAGCCTTCTGCTCTAATAAAGTAAGATAAAAGCATTGAAAGACCTTGAAGTATTGTTCCTGAGGCATAAATATAAACATATTGTTTTGCTCAATGAACACTAAGATGTCTTGAAGCATCATAAAATGCTTGTAGTGTTTCATTGTCTTCTTTAGTCATTAAGGTTAAATTTCTAATATTAGTTTTACCTGCAAAAGCATCAACAAGACCCGATCATATAAAGATAAATGACATTAAGGTTGCTAAGAATGATAAAGCTACTGTCATTCAAAACATAGTTGCTCACAAGTCTCTTCCACTTTTTTCTAATTTTTTAGAAATACATTTTGTGTAATAAATACTTGCACCAACAGGTACTAAAAATACTATTGAGTTAGAAAAAATAATTAAAGGTTGAATTGCAGCTGTGGTCATTGAAACAACCCCATTAGCATCAATTTTTCCTAAATTCAGATCTTTATAACTGGAGTTATTTATGATATCCAAGTATTTCTGCTTCAATTCGTTTGAAGTTAAAGAGTTTAAAGTATCTGGTAAAAATTGAATCATTTCGCTTCAAATATTTCTATTATCAGGAACAAATTTTTGTAAGAAAACTTGATCAATAAAAGTATATAAACCAAACATTAAACTACTTAATAAAGAGGGAATGGCAACTATTCAAATAGCTTTCTTTATTGGAGTATTTGCAAATAATTTTTCTGCTCTTTCCTGTTTAGTTATTTTTGACATTTCAACCTCCTTTATATGAAAAATAAAAATTCATAATGGAAAAATCATACCATTATAGTCATTCTATTAAAAATAAAATTGGTGTTTAATAAAACAAATGGTGCCGACAACTGGACTTGAACCAGCGACCCCATCCTTACCATGGATGTGCTCTACCGACTGAGCTATGACGGCTAATTATGTTTTTTATTATAACAAAAATCATAGTATTTTTAAAAAAATAATAAATATTTATTGGGTAAATTTTTAAAAATATTTGCTATGTATTTCAATATTTTTAAAATTAATATTTTTACGAAAATCACTGTTTTATAAGCTTTTTTGTAAAATTAATCAATCAAAATTTTAATTAACATTTTATATATATAAAAGTTATTTAAAGAAAATTTAGCACTCTCGATATAAATGTGCTAAAATAGAAATATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAGCTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTTAGCAAGTTTACTCAAAATATTTTTGATACAGTATTCAATTTTGGAGGTGGAGCTTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGCGATGATATTTACACTGAAATTGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTTGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATGTTAGAACTTGTAATGTTTGTCAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAATATAAGCCCTAGCGGCTTTTTTTATATAATTAAATCATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGAATCGATTTAAAAAATAAAGTTGATTCATATGACACTCAAAATTTTGATTTATCAAACAATTTTTTCAAGAAAATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAACAAAAGTTGTTTTTTTATTTTTCTTTTATATATAAAAGCCTTTTGATTTTTAAGGAAAAAAGTTGCTTTTGTAATTAAATTTAAGTATAATACTAAATATTAAAATATAATTAGAACTATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGAGCAATAAAAGAAGCAATGTTTAAAGTTGACAATTCTTTGTTCGAATTTAAAAACATTAAACTTCAAAAAGAAAACGAAAGTTGACAACATAAAAATTTTGATATTTCAATTTCACATGAAGATGACTATTTAGTAGCTGTGGTTTTGAAAAAGAAGGAGTAATATGAATTATCATTTAAAAATGTTTCTTACATGGTGAATATGATTATGAAGGATTTATCGGATTAGTGTTTATGCGCGTAAATATCGTCATACTCCTGATTACTATACACCACAACAAAGATACGATTGATTACTTAAAAAAGTTAAAAAATATTTAAAGCTATACAACATTAAAGTTACTGTTGAAGGTTATGACAATTTACCTAAAGCGCCTTGTTTATTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGAAAGAAACAAGAATTCAAAGTTAAGTTCTTGAATCCAATTAAACCAAGTACATTTATGTCAATGGACCCAGGAGCTTTAGCAGATAGAGTAAAAAGTCTTATGGAAAGGGAGCTAGAAAATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGATATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACCAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAATTTAATTAAGGGTGCTATTTTTAGCACCTTTATTAATATTTAAGAAAGGCAAAATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTAGCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGTGACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACAGGTTTTTTTGAAAAAATATATAAATTAAAAAGTGATTTTAATGAAGTTAAAAAACTTGTAGAAATTAATATTCAGCATGATGAAAAATTACACAAAATGGTTGTTACAAATAATGCTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATGAAATTTTTGCATCTATTTTAACTGGCAAAAAACATCAAATTAGATTAAGCATGTCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGACGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGATAAGGAGAATTATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGACCAAATTATGACTGAATGAAAAGTTTTGAACAAGAACTTAAATATGCTTAAAAAATTAAATACAACAAATGTTAAACCATTAAGTCACATCAATGAAGAAGCTTTAGTAGACTTCTTAAGAGAAGATGTTGAAGACACTTCTTATAGTATCAACAAACAAACTGCATTAAACAATGCTATGGACAAAGATCAAGATTACATTATTACAACAAAGGTGGTGAAATAATGAAACTACAAGTCTTAGGTGATTTTGATTTAGCCCAAGCTGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGCTTTAAAAGAAGGCGATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCAACTAAAGATGCAAAAACAACAGCTTCGAGCTTAATTTTAGAAAACTTCAAACCTGGCTACAATGCAACCTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAATAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGAGAAGAAATTATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGAGATAAATTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGAATTAAAAAAGATAACTTACCAATTAATTTAGCTATTGATGCCGATATTTATCAAGACGAAAAACTTCTAAGTCATGCTCTTTGAATGGAAGAGTTTTTAGGAGGTGAACATGAATAATTTTGAAGTAATTATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATAATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGAGAAAGAGAAGCTAGATACAAAAAAGAAAATATTCAAGATATTTACATCAACAGTTTATTAAATGATCTTGATTTAGCAAAATATTTTGATTCTATTAATTATGTTGATAGAGATAAATTATCTAAATTATTTTTTGCTGAAATAGTTTCACTAGCAAATAGCAAAAGCATTCATCCAACTGAATTAAATATTAAAAATGGACACTTATTAAAAGGAATTGAATTAATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGAGTTAATGAAATTATTAGCCAAAATGAAAGTGTTATTGCAGAATATCCTAATCGTGAAGAAAGAGTTCTTAAATTTGTTTTAGGTGCTTTAATGAAAGTTTCAGGCGGACAAGTAAGTCCAATTAAAGCAAATGAAATTTCAGTTAGAATTCTTAAAAACAAATTTAAATAATCAATATAACAACCTTTTGGATGTTTATTAACAATCAAAAGGTGTTTTTTTAATTGTTTTGTGTGTTTCACACATAAAAATGTTATAATGTATGTGTAAAAATCACAAAAACACAAAAAGGAGAAGAAAATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAATATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGAAGAACAACAGTTGATTTAGATTTTATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACATAACCAATAAATGTTAATAATGAAAAGAAAGGAATTAGATTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGAAATTCAAGATTAAAAGACTTTTTTGATATTTATTATTTATTTGAAAATCAAAAAAAATTTATTAATAAAGAAATCTTAAAAAAGGCCATTGACAATGCTTTTAAAGTTAGAAATGCTTCATTTGATAAAACTATTTTTATAGATTTACTAAATGCACTTAAAATTTCAGAATTACAAAATTTACAATGAGAGAAACTTGTTTTATCTAAAAATTGACAAACATCAAAAAGCTTTGGTGAAATTGTAGAAGTAATAATTAAACATTTTGAGAATGATATTGAGAATGTTTAGAATTTTGTGTAAAGTATTTCACTTTCTTAATAGAAAGGAATTGATTCAACACAAAATTTTTTATAGCCCCCCCATTTTTTAAATTCTAGAATTTAGGGTTTGTTTATTATTGAATTATTTAAGATTTTTAAGGACTCCATTTTTTAGATCATTGATGCTATAAAGATGTTCGATGATTTCGAAAGCTTCTTTCAAATTGTTTTTTGCATTTTTTCAACCATTTATTCCATCGGTAATTCAAATGAATTCAAAATTAGTAATTGTTTTTGTAGATAAATAAATTTTTTCATAGCTTCTTGAAACTTCATTAAGTTTAGAACCTTGAGCCGTATAAAAATTTACTTCAATTGCATAAAATTTATGATTTTTAATGATTCCAAAATCAAATCTTTTATTTGCAGTTGTTTCTTGAATATTATTTAAATTAATTCCAAACTTTTCATAGATCTCACTTGAATATATTTGTTTAAAATATGTTTTGTTTTCAACATATCCAGCTTGTTGAATATATTTTTCAACTAGTTTTTCCATTTTAAAACCTGTTCTGTTTTTTCTTGCATTGGTGTTGCTGCCTGCTTGAACACCATCATAATAACCTAAAACATAATCTGGACTTTTATTTTTAAGTCAATCTTTTAGACCTGTTTTTTCAAAAAAGTCATTGTAATCTTTAAAGTCATTTGTAAAATTCTTAAAGTCTAATTCTTTTATTTGCTGATTATCATAAATTTGCAAATAATTATTTCTTACAGCTAATAGACTTGGCAATATTTTTAAAACTTGTTTATCATTATCATATATTTTTTTAAATTTTTCAGAAATATTCTTGTCACCTACAATATTGCTGAATTTAACTAGTTCTTTTCTATAAGCTTGTAAATCTTGATTAATTTTTTCAAAATCTACATAATAGTCATAGCCAACTATTGTTGGTGTTAAATTATTAAATCACTTTTCAAAATCTCTTTTCATTTTACTCCCTATAATTTGTAATTATTACTTCTTCAACTTCTCCTCGTCCACTTCCATTTGAATTTATCATTCTTTTTGCTTTAACAACATTAATATTAAAGTCTGCATATAATTCATTAATATATTTTGTGTTGTGATTACTTAACATTACTTTGACACCTTTTTTGTCAAGTTTTTTAAAAACTTCGGATAATCGTTTTTGTTCTTCTTTTCCAAAACTTTCTGAATTATATGAAGTGAAAGTATTTTTGTTTTCAATGACATCATAAGGAGGATCAAAATAAACAAAATCTCCTTTTTGAGCTTTTTCACAAAATACTTCAAAATCTTCACAGAAAATTTGATTATTTGTAGTTTTTAAAAAGTTTTTTAAATTTTCAAAATTGTCTCTATCAAAGCAATTAACTGAGTTTTTCTTGCCAGAAGGTACATTAAATTGTCCTTTAGAATTTACTCGATATAAACCATTAAAACATGCTTTATTTAAGTATATTGTTCGGGCAGCTTTTTTGTAATTTGGCAGTTTATCGTATTTATCTAATTTGTCCAAATCTCTTATTTTATAGTAATACCCATCATTATGATTGTTTTCATGATTAATTAATTCTTTTTTTAATAATTTAAAATCTTTATCATTTTTAAAGCAATTAAAAACATTAATTAATTCTTTATTAAAATCATTTATTATGAAATTTTGCGGCTTTAGATTTAGTAATAATGCTCCGCCACCAACAAAAGGTTCCAAATAATTGTTATATGTTTTTGGCATTAAACTTTTAAGTTTATCTAATAATTGAGTTTTGCCACCAGCCCATTTTACAAATGGTGAAAGTTTTGTGTTTAATTTTCCAATATAAATATTATTCAAATTCAAAGCATTATATATTTTGCAAATTGTAAAAAACTGAACGCCCTTAATTTGACCTTTTTCAATTTTAGTTAATTGAGATCTTTCGGTGCTACTTAATTCTGCTAATTCTTGTTGTGTCATATTTTTTGCAAGTCTTTCCATTTTAATATTGTCGCCAATTGTTTTAAAAAGTGTTTCCATTTTGTGCTCCTTAAATAAATATATTTTAATTATATCAAATGTGTTGTTTTGTACACAAATTATTTCACTTTTTATTTTAAAATTTTTCAATATCCTCTTGTCTTACCAATTCTAGCTATGTAGCCCTTGTCTTTTAAAAGATCTAAAATTCTTTGAATAGTTCTCGAAGATTTTTTTATTTTTTTAGCTAGTGAACCAGCTGTTTCTTTTGGATTATTTTTTAAAGCCATATATACATAATAAGCAGAATCTGGCAATCAGTATTCTTTAAATTCTTCATCTTTACTAATTAAACTTTTGTTTTCTCTTAAAAATATAAATGCAAAACCATAATTGCCTTTTTGATAAGTAATTTTAGCATTAGTTTTTTCACATTCTTCATATACTCTCTTGAAACCATTATTACAAATTTTAGCTTCATCAACTAAATTAATCATTTTTAAAATTAATGGATTTCTAATAATTGATTCTATGTTTTTATATGCAAAATCTTCTGGAGTGTATTCAATTGGAAATTGACCAGGATTATAGATTTTAATATTACTAGGATGAATATTAATTTCGTGTTCTATTGCAGATTCATATTTTGCATGTGCAAAACTATTAATAATTATTTCCTTTAAAGAATCAATTGGTATTTCTGGAATATCAATTCTTTCTATATCTACAAATTCTGCTTTTCAATTGATTTTAGATTTAACATATTCATTAGCTTTCTCAATTAATTGAAAGATGTTACCAGAAATTCTATTGATATCTAAAAATGTTAATTTTTGATCTGTTGCAAAAACAGCCATTTTTAAAACTATAGGTTCTCTATGCGAAAAAAGATAATGAGCAGCATTTGTTAGTTTTTCATTTTTCATCAAACTTAATTTATTTAAAATGTTTTCAGCATTAAATTCTATTTCATTTAATTTTTTACTTTTTATAGCTTTTTTATAAAACTTTTGAAATGTTTCTAAATCAATATCATCAATTTCATAATCGCTTAATTCACTATCTCAGCTTTTGTTTTTATTGAATTCAAAAAGCTGTCTTAATTGATTAGGACTGAGTATTTTATCCTCATCATCATTTCTAATATAATAGATGCCTTCAACTGAATATGGAGCATCATTCCCACAAAAAGTTACCTCTATTATTTGTTTATTATCAACAATTTTTTTCTCAATAATAGGACTAATTACAGGTTCAATATTTTCATAAATTTTTCTGCAAATATCTCTTAAAGTATTTATGTTGATTTTTTGATCATATACTAATCCATCATCTCCGATGCCAAAATATAATGTGCCTTTTCCGTGTTTATTTAACATGGCACAAATTGATTTCATAGCCTCATTTATTTCATTTGTATTTTTCTTAAATTCAAGTGTTTCGCTTTCGATTCCAAAATTCATTTTTAACCTTTTTTTAACTTTTATAATTACATTATACATAAAAAATTTATTTAAGACAAATTTATGACAAAATTTATGACAAAAAAATAAAAAAATATTTGTTCTTATTGTGATAAAATTAAAATAATTAACATAAAAAGGGACAAAAATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTTATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGAGAAAATATAACAAATAAAATTAGCGAAGAAATGGGTAGTGAAGCTGTTAGATTTTTTAAAAATATTGAAAAACTAAAAATATGAAAAGATGATCCTAGATTAAAAAAAGAACGACGTGGAATTATTGATGCTATTGAAGAATATGAACACAAATTAAGTGATGAAGTTGAAGAATACTTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACGTGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGAGTTGACTATATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGACGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGAGCTAACTACGAATATGATTTATATAATGCTATCGAAAAAGATCAAGCTAGTCCCTCATATACTTCATTAAGTAAGATTTATCATAAGAATTCTATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGAATTAAACTTGGAAAAAAGATATTTAAAGTTAAATAATTTAGTGAGCAAAACATGCTCATTTTTTATTCAAAATTATTGAAAAAATCAAATTTAACATTTTTTATATCGAAAAGTGTAAATTTTGTATTTTTACAACTCAAAATGTTGAAGCAAAAGACAAAATAACTTATTATCCAATTTACATGTCGATGTTTTTAAATGATCAAAATATAAAACTTCCTAAAAATGAAAAACATCAAGATTTAGGCGTTTTAACCCAATATTTATGATTTTTTATTTGAAATATGTTGCATATTTTCTCAATAAAGCTCATTATAAGCAATTTGTCTTATAATTATTTTTATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGATTTTTGGGATCTGCCACTGTGCCTTATTTAGGTTACATTCCTATTGGGCATACTCAAATAACCTTTTTAGCTTTTATAGTTGTTTTTGGAACTTTTCACTTAGATTATGTTGGTAATCTTTCAGCAGGATTATGTTTTGGATTTAGCTCATGAATTTTAGCTTTTATAATGGGGGCGATTAAATATCAAAACTTTGATATCGCCGTTATTCCTAGAGTTTTAATGGCAATATGTGTTTATATGCTGTATGAAATTTTTAATGTTAAGAACAATATAAAATGTTGAAAATTTATTATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATAAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGAATAATTGTTCATCCAATTAATATTATTGGCGAACCTATAATTTGTGGAATGTTAGCCTATTTCACTTTTCCCTTAATAGTATTTTTAAGAAGAAGATTCGAACAAAGATTCAGATATCATTGATAAAAAATATTAAAATATAGATCTAAAATATCGTGAAAAACGGTATTTTTTTAAATACCTAAATTTTTAAACTAGTAAAAAAATGTTTATTAAATTTAATTAATATATTAAAATTAAAATACATTATTAGTTAACAAATAAAATTTAAGGAGAATTATGGCTGAAAATAACAAGAAGTTTACTATTGATGAAAAAAAGAGTGAATTAGTTTACACTCTTGAAATTAATGGCAAAGAATGAGAAAAACTTTACGCTGATGCAAAAGCCAAGGAAGTTAAAAACATTAAAATTCCTGGATTTAGACCAGGTAAAGCACCACAACATGAAATTGAAAAAAGAGTTAATCCTCTTTCTGTAGCAAGTGAAGTAGTTGATCAATACTGAAGAAATAATCACGATCGCTTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGAATCGCTCAACAATTAGAAAAGAATGCTTTATTATTACCACTTAAAAAAGATGAAAAAACAAAAGATGGTGACACTGTAACAATCGATTACAAAGGTTTTATTAAAGATGAACCATTTGATGGTGGTGAAGCTCAAGGTTTTGATCTTAAATTAGGATCACACACATTTATTGATACATTTGAAGATCAATTAATTGGCAAAAAAATTGGTTGAAAAGGTGAAGTTAAAGTTACTTTCCCTAAAGAATATGCAGTTGAAAAACTTAAAGGACAACCCGCTACTTTTGAAGTAGAAATTAAAGATGCAAAAAGAAGAGAAAAAGTTGAATTAACTGACAAAAACTTTGGTACAGTTCGTCTAGCAAAAATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTGTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAATAATTACATATAGGCACATAATAATTAATCAGGCAATTTGCTTGATTTTTTTATTAAACTTTAATTACTTATTTACTAATAACAATATTTATAATAAAATTAATTTATTAATTTAAGGAGAAAAAAATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCGTATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGAATAATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGTTAAGTCTATATGAAGTTCAAAAAGGAAAGGACTTCTTAATCAAATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAATAAGAGGGTTTATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGAAATTATGTTAAGCAATTAGAAACTGAAATTGAATCATTAGAAGAAGCTAGAAATGTTGATCGACAAAAATTAAATAACAAAATAACTGAATTGGAAAACAAACAATTTAATGAATTACTTGATACAAACTTAAGAAAGAAAATCAAAAAAAATGAATAATAATGAGCAAACTTTAATTAATTGATATCCTGGTCACATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGACTTGATTTAAGACAACTAAAAAGTAGAAATCAAATCTTAAATCAATTAAAAGTTTTATCTCAATCTAAGATTAAAAAAGATAAAGAAAAAGGTTTTTTAACTAGCAACATAAAAGTTTTTGTATTAGGAGTGCCAAATGCTGGGAAAAGTACATTGATTAATTTAATGAGTCAAAGGAAAAGTTTAAAAGTAGCAAATTATCCTGGAGTTACCAGAGTTAAAAAATGAGTTATATCAGATAATTTTTACTTTATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGAATCAAAAACTTAAAGAATGTTACTTTTGATTAGGAGAAAGTTATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTGAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGACAAAATAAAGCGGCACCATCAATCACATATACAACTTTACTTTGAATTTTAATTTGTGTCTTTTCAATACTCACAATTCTTTCTCTTTATTTATTAATTTTATTTTTCATTGAATATAGTTTAATCAAAAAAATAGGTCTTAAAAAATCAGAACAAGAAATAGAAGCATCTATAAGAAAATTTGTTAAATTTGGATTTAAAAAATATCCTAAAAAACAAATGGAAATGCTTGAAAAATTCTAAAAAGCCTGTTTTATAGTGCTTTGGTAAAAATTGGCAAAAAAGAAAAATTCCACAAAACAATCATATGATTGTTTTTTTAACTATCTTTAAAGTATAATTTAAATATAAAAAACAAAGGGGGATTTATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTCAAAACATTGCATTATTAGGATCAAATGGAGCTGGTAAAACCACAACAGTTGAAATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAACTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGTCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATAAACTTTATTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTGGCAGCTTGCTTAGAGTACTACATTTAAGGAGAATCATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGAAAAGCATTTGTTGGTCCTTTCTTTGCTTTTGGTTATCCTATAGTTTTTGTAATTATCTTAGGTGCTGTCTTTAATTATGAAATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTCGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTAGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGCTATTTGAACAGCCTTGGTTGCTTTAATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGAGCTGGTTATATTTATAGTGCTATTGTTTTAACACTTGTAAGTATTGCTGTTGGTTTATTTATAGTTTCAGTAAGTAAAAGTATTTTAACTATCCAAGCAATAGGTTCATCATTATTAATTATTTCAATGTTTTTAACTGGACAAGTGTTGCCTATATCTCAAATTATTGGCCAAAAAGTAATGTGATATTTATCATACATAACTCCATTTAAATCGCCAATAACTCAAAACACAATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGAAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGACTTTGAATAGTTGTTTTAGTTGGCTTATCTCAAAAATCATTTACTTGAAATACAAGATAATAAATTATTACATGCTCAGTCATGTAATTTTTTATTATAAAAAATTGGCTTAAACCAATTATTTTATCTTTACTATTTCTTCATTTAATGATTTAAACATTTCATAGCATTTTTAAATATGCAGCAAAACCTTCTTTAATCTTTTTTTCAGCAATTTTGACTTTATAGACTAAATCCATATATTTATTAGCTGTTGTATGGTTTCTTTTTTCAAGATCTTCTTTATCATTAGTTAAATCTATTTGAATTTCAAGTTTATATTTTTTAAACAAATTTTGAACTATTTCTTTTGTTAGTAGTTTTCAATTTTCATTAAATTCTTGTTTAATAAAAGGAAAAACCTTTTTGTTATTATACAAAGCTTGATTTTTTTAAAGCTGCTTATATTTATCAATTTCTTTAGCAGGCTTGTCATACATTCTGTCTTCTTTAATGCATGAAGCACTGGTTAAAAGTAAGGTTGCTAAAGTACTTAATGCACTATTCATTCTTTTCCTTATATATTAATATTATAAAAAGAAAACTTGAGCAATGCTAAATGTGAGAATAATTATCAATTTGTTGCATTAAAACTTTATAATTAAACTAATAAAAAAAGGAGGTTAGTTATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAACCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAAGGAGTTAATTATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGTCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGTGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAGATTTCCGAATAACACTGTTTTATAGTTTTATTAGTTAAAAATGAAATAAGCATTTGAAAGTTTATTTTAATAGTAATATTAGTTGCTTTTTTAAATATAACTTTAACCTTAAATTTCTAACATTTACATAACAAAATATTTGTTAATTTAAAAAAAATATTACCAATCAATAATTCGCAATAATTCACTCAATTAACATTATTGGTTAGCAAATAATTTTTGCAGTAGTAGAATTTGCTTATCCATTGGCTAAATTTTTAGGAATATAATTTGTGCAAAGATTAGAATTTCATTGATAAAAATAAAGTTATTGTTTATAATGTTAAAAACTAATGGAGGAAAATTATGAAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGAGCTAAAGAATTTTTATCAAAAGACTGAGATAAAAACAAGAATACAAAAAAAGTAATTATTTTTAATTTAAAAAGATTTTTAAAATGAACAACTGAAGAATTAAATAAATATCAATAAATAAAAATTGAATTTTAAAAACCGGTATACAATGATACCAGTTTTTTAAAATTTTTTGCCAAATATACTACGTTTAGAAACAGTGTATGAAGACACATAAGTTTTGTAATATGCTTCATTAACTATAATTCCTAAAAACATAAAATATGTTAAGAATGAAACAAATAATGCAATATACATAAATGAGCCTATAATACCATATTTGTCATAGTTAATTAATGATGTTAAAAATCCAAAAATAGTGATAAAGATCATGACTGGAAAGCTAGTAATTAAAACGCCAGGAAATACTGTAGTTCAACTTAATTTAAAGCTAGGACTCATGGTATAAAGTAAAGTTAAGCCAAAGTAAAGCATTATTAATAAATAGAAAGAAAATGTTATATAAAAATATATATTTTTGTGATATGTTGACATATTGTAAGTAAAAGTTTGATAGAAGAATAGATATAAAGCAGCTGCTAAAAATAAGTAAATACTGATACCTAAAACAACAAATAATCCTTTAATTCTATTAGTAAATCAGTTACCTAAATTTTCATGTCCATAAATATAATTTTGACTGTAAATAAATTTGCCAAAACCACTTGAAGCCATTCATAAAGAAGCCAAACAAAGAATAATTAAAGAAGTTAATTGAGCGCCTTTTTTTGTGGTGTCAGGGATCTTAATAATAGAAGTAACACCAGGAATAATTCGAGTTAAAACTTCATTAATAAATAAAGTATGATAACCAGGAATTAACGAAAGTAAAACAATCATAATTGTAATAATAGGAACAAAGGAAACTAAGAAATAAAAAGCAGTTGAAAGTGGAATAAAAACAAATGATTTATCAGTAAATTTTTTGTAAGTTCTATTAATTAATTCGTTTGTTTTGCTTTTGTTTTTTCAACTTAATCTAGGGGTGGCTATTCACAAAATAATCATGATAAAAAACTTAACTATTTTTTCAAAAAATAGTCATTTTTTATTTTCTACAGGCACAATATTACGAGCAAATCAAGATTTTTTTAGACTGTTTCGGTATTCTTTTTTAAGTCTATTTTGATCTAATCCTGTATAAATTTTATTTTGATTATTATTATTTTGCATATTTATAAGATTATATATTAATTAAATTAAGCATAAAGATTAATTTAAATTATTCTAAACCACTTTTGTTTTTGAAAACTAAATTAATTGGACAACCAGTGTAGTCTAAAACTTGTCTAATTTGCTTTTCTAAAAATCTTTCATAGCTAAAGTGGACATATTTTTTATTGTTAACAAAGAAAACAAAAGTAGGAACTGGATCTTGAGTTTTACGAGCAAAATAAATATTTAAACGGCCACCATTATAAGGAGGAGCTGGCTGAATCATTTGTGTTTCTCTGATTAAGTTAGAAAGAACTGAAGGTTTAACATCACGTTCAAGATTTTGTTTAACTTTAATTATTGTGTCAATTAATTTGTCCATTCTTGAGTTTGTTTTAACTGAAACAAAACAAATAGGAACCCAAGGTACAAAATGAAATTTGTTACGCATTTTCTTTTCGAATTCAACCATTGTTTTAGTTTCTTTATTAATTAAGTCTCATTTATTAACCACAATAATTATTGGTTTATTGTTGTCTAAAGCATAGCCTATAATTCTAGAATCGAAGTGTGATAATTCTTGAGTTGAGTCAATTAAAACTAAAGATAAGTTAGATTCGTCAAGTGAACTCATAGCGCGTTTTAAAGCATAATGGTCAACGCTATCTTCAATTTTACTTTTTCTAGTAATACCAGCTGTATCAATGATTTCAAAAACTTCATCTTTGATTTTTACAATACTTTTAACACTATCTCTAGTAGTTCCCGCAATATCAGAAACTATTGAACGGTTTTCATTTGCTAATCTATTTAACATTGAACTTTTACCTGAATTAGGACGTCCAATAATTGAAAGTTTGAATAGTTTGTCACTCTTTTCATCATCAAGTCTAAGATGTTTAAGACATTGGTCTAAGACATCACCAACACCATTACCATGTTGAGCTGAAATAGGAAAAATATGATCCACGCCAAGTGAGTATCATGAATAATTAAACTGTGAAATATTATCTAATTTGTTTGCAACTACGACAATAGGTTTGCCAGATTTTTGAAGCATACTGTAAATCATTAAATCGTCATTAGTGATGTCAGAAGCACCATCAACCATAAAAATAATTACATTAGCTTCTTCAATAGCAATTTTTGCTTGAATTTGAATATGTTCTTGAAAAGGTCTATTTTCGATTTCGATACCACCAGTATCGATAACTTTAATTTCTTTTCCATTTCAAGAAAAGGTTTCATAAAGACGGTCCCTTGTTACTCCAGGACGGTCATAAATTATTGAACTTCTTTTACCAATTAGCCTATTAAATAGGGTACTTTTACCAACATTTGGTTTTCCAATAATGGCTATAACGTTGTTTCTCATTGATTATTTATTAAGTTTTTCTATTGTTTTTTTAACTAGTTCAATAATTTCAACAACAACTGCTTCAAAACCCATATTTGTACAGTCTATTTCTTTTGCTTCTTTAACTTTATGAAGAGGGTCTACTTCACGATTCATGTCTTGATAGTCTCTTAATTTAACTTCTTCAAGAACTTTGTTGTAGTCCATATTAAATCCTAAAGCAATGTTTTGGTTTAATCTTCTCATTGCTCTTTCTTCTGGTGTTGCTGTTAAAAAGATTTTTAATTCTGCATAAGGCATAATTTTGAATGTAGTATCTCTTCCATCCATAATGTAACCTTTACCTTTTTTAGTAATATTTTGAATTAAATTAACAACATATTGACGTACTTTTTTATATGTTGCAATAGTTGAAGATCCATAAGAAATTGAATCTTCTCTTATTTCAACAGAAACATTTTTTTCTCTTAAAAAGATGTTTTCTCTTTCATCTAAAGTGATGATATCTTTTTCTAAAGTTTTATTGATTTCTTCTTCATTATTTCAATCAACACCTAATAATTTTGCATTTAAAGCTAATGCTCTATACACACTTCCACTATTAATAAATGTGTATCCAAGCCTTCTTGCTACTTCTTGTGAAACAGTTGATTTACCTGCACCGCAAGGTCCATCAATTGCAATATTAATCTTTGTAATTTTTGTCATAGTGCTCCTTTAACTAATAAAATATATTTATATTTTAATATATAAATAAGAAATAATAAAAAAATCACGTAAAGTGATTTAAAAATTATTATTTGTATTCATTAATTAATAGCACTGTATCATTTATAATTTGCTTCATATTGTCAATAGGTTCAAGAAAATTTCTTTTAAAATTTTTATATTTTTCTTCAGCTTTTTCAGTTCCTTTTTTGTCTTTTTCATAGGGCAATTGGGAATTTTTGATTAATTTTTCTAATCTATCTACAAAGGTATATAAATCAACACTTTGAAGTTTATAATTTGAGTTAAAAACAATCATAATATTTTTTGCCTTATATTCTCATGAATTATCAGTTATAAATTTTTTAAAATCATTAATTCATTCATTTAATTTATTAATTCTGTTGTTTTTATCTTTAGGAGAAATAACTGTTGATTTTCATTCTCTTAATAAATTTATTGCAGCATGAGTATGTTTTCCATCACTCTCTGAAGAAGGATTTTTAATTGTGCTATAGTCAATTGACGCTTTGTCTTCATCATAATAACGATTTTTATATTCACTTTCAATTTCTTCATTGTATTGGTTTATTCATTTTAAAAAGCGATCTTGATATCATTTTTGTCTAGATGTTTGATTTTCAAAAAAAGGTCAACCGTTTCAGTGCGAATTTTCAAATAAATTTTTAAATTGAGATAATGTAGAATCATTTTTTATGTATTTAGATCAGTCATTTAAAATTGAATTTATTTCATCATTTATTTTCTTCAAATAGGTTACATTTTTATAAAAATTAAAATTAGTATTATCACTAGGAGTAGATGTAAAGTCTTCAAAATATTTTTTGAATTTAGTACTTTCATCAACATTATTAACCATAAATTTATATGCTTTTTCTTCAAAAGCTTTTGAGTTTTTTAATAAAAAGTTTTCAAATGTGAAATCAGTTATTTCTTTAGTTTTTTCATATTCCTTATAATAAAATGATTGATATGACAATTTGTGGTTATCCTTGTCCATAAATTTAACTAATTTATTAAAGTTATTTGTATAAATAGAGACATTTTGTTTTAGATTGCTAACAAAATTATTTTTTTCATATTCACCCATGTCTCAAAAACTACTTTGACATGCAGCTGATGTCAAAGGAGTGGTTGTGGTTAAAACTGTAAAAGAAATTAAAAATTTTAATTTTCTTCTTTTTGACATAATTTCTTTTCTTGCAATGAAATTAAAACTTTAAGATCTTTTATTTCTTTAAGAGTTAAAGTTCGATATGAGCCTTCAGGCATTTTTTCAACTGTTAAGTTAGCAAATGACACTCTTTTAAGATTAATAACTTCATGATTTAATGCTTTGAATAATTCTTTGATGTGATGGTATGAGCCGACATGAAGAGTTACTAAATAACTTTTAGTATCAACTTGGTCAACTATTTGTTTTGAAAACTTACCATTAACAATCAAACCAGCGTTAATTAATTTGAATTCTTTTAAGCTTAAAGGAGAATCAATTCTAACACGATATGTACGTAAAATTTCGTATTTAGGATGTGTTAGTTTATTAATCATTTCTCAATCATTTGTTAATAACAAAGCGCCAGTTGTATCATAGTCTAAACGACCTACAGGAGCAATTCTTTCTGAGTAATTAATTAAATCAACAACTGTTTTTCTGCCCTTAGGATCTTTAACTGTTGAAATTGTCTTTTTAGGCTTATTTAATAGGAAATATACAAAATTTGGTTTTTGTAATGAGATTAGTTGATTGTTAATTTTTATCTCATCGTTTTCACTTGCTTTGTCACCTAATTGAACAATTTTTCCATTAACTTTAACTAGTTTTTTTTCAATATATTCTTCAGCTTTTCTTCTTGAACAATATCCTGTTTGTGCGATTAGTTTTTGAACCCTAATCAATTCTTTCATTGCAAACCTTTCTACCAAAAAATTTATTTCATTCATTTGTCATAAATTTTTTGTTGTTCTTGTGTAAATATTATACCTTTTTCTTGGTTATATTCATAATTGCGTTTTACAACTTCTTTAAATCCTTCATCGAAGTTACTTAATGCTAGTTGTCTAAGCTTTTGAACTCCGCTAAAAGCTCGACCATCACAGATTTTATCTGAGATATATAAAATTTTATCAAGAGGTCCTAATTCTAGATCTAAAGTTGTATGAACTTTTATTGCTTTGACTATATCTTCATTATTTATTTGATAGATTTCTTTAACTCATAAAGCTCCACAAACTTGATGTAAAACATGATTTGGATAAATAGATTTGTTGTTAGCTTTTTTATCATATTGAGCAATAAATTTTCTACTTTCATTTTCACTTAATTCTTTACAAATATCATGAAATAGTCCAGCATAGTAAGCCACTTTTGCATCATATTTTATTGTTTTAGCTAACTCAGCAGCAAAAGTTGCTGCAAAAACTGAATGTTTTGCTCTTTTTGCACTAAGCAAAGCGTGAACTATTTCAAAAGCATATAAAAAGTGACTTCCTATATATTGGTTAACTTTTTCAGGTACCATTCAAAGTTGACCTTTTCTAACTTCAGTCGAACTTTCTTCAAAAAGATCATTGTTTAAAATTTTAACATTGAATTTTTTAGCATTAATTTTATTAAAATTTTTACTTCTTTTAAATACAACGAATTGAGCTGTTTTTGTTATTTCTTCGATATATTCTCATTTATTTAATTTTGGCAATAAATCGCTGCCTATTAAGAAATATAATTCATCATTTGGATATTGTTGTTTGAAATATCTAATTGTTTCAAAAGTATAACTAACGCCACCTTTTTTGATTTCAAAATCATGCACAGCATAATTGCCTTCTAGATTTTCAATAGCTATATTAATCATATTAACTCTGTCTTTATTAGGGGCTACTTTATTATTCTTTTTAAATGGGCTTATCGCTGTTGGTATAAAAAACATTTTGTCTAATTTAAGTGTTTTATAGGCATATTCAGCAATTTTGATATGTCCACTATGGATTGGATTGAATGATCCACCAAATAATCCTATTTTCATAATTCTCCTTAATTTTTATATTAAATTATAAATTCTTTTTTGTAATATCCTCTTGTAAATATCTCATTTGTATATTTTGTTTTTTCTTTTCTTTACTATACTCTGAAGCCAATTTTAATTTTTTGTCACGCAATTGATAATTGACATCGTTAATAATATTTTTAATTTTAATAGTTTGAGAGCTTTCATCTTCAGGAGTTGTAAAAATACTTAGTTGTTTGCCATAATCAAATTCATTTACTAATTTATGTAATCTAACGCCAATACCTCTAATAGGTTCTTCATTTCACAATATTTCAAAAAGTTTACTTGCTTCTTTAAAAATAATATCTTCATCATTAGAAACAACTGACATTGTTTTTTGTTTAGAATGTCAAGTTTTATTTGTGTCTCTTATTGAACATGAAATGACTTCTGGTAAGGTATTTCTATTTTTTGCTCTTTTTGAAACTTTAACACATAAACTTCTTAGAATGAGCAATATTTCCTTGCGATCATCAATATCATATGTTAAAAAAGTAAGTTCATTACCTATATTTTTTAAATCATTATGTTCATAATTTAATTGGTCATTGCCATTGCCTTGAGCTTCAAGAATTATGCCTTTAGTTTTAACACCAAAAATTTTTTGTATTTTAAAATTTTCTCAATTACATTTTGCTAATTTGCCAATAGTATCAATATGCATTCTAATTAATCTCTCAGCTGTTGGAGCACCAATACCGAAAAATTCAATAATTGGTAAAGGCCAAATCCTTTTTTCTATGTCTTCCTCTTTTGTTTCTAGAATTCCAAATGGTTTACCTAAATTAGTTGATAATTTTGCTAAAAATTTGTTGTATGAAATTCCAATTGTTGCAGGAATGTCAAAAGTATTTAATATGTCACTTTGGATACTTTTAGCCAAATCTTTTGGATCTCTATTTTTGATAATATCAGTTACATCAAGCCAACATTCATCAATTGAAAACACTTCGATATTTTTTGTATATTTTTCACTAATAAATTCAAAAATTTTATTTGATAAAGATAAATATAAATCAAAATTAGGTTCAATAAAAACTGTGAGAGGATCTATTTCTAAAATTTTATTTTTAGGTCAGCCAACTTTAGCGCCTTTTCTTTTTAACTCATAACTAATTGAAGAAACTATTGATCTTTTTAATCTTCTACCAATAGCTACTGATTTTCCTTTTAATTCAGGATTAATTGAGCGATGAGCACTTACAAAATATGAATCAAAATCCATGTGAAAAATTGTTTTTTTATTTTCCATCTTGCAATTTTTCCTTTATTGCTCTAGCTAACATTAAAGCACAATTAACGCGATTAAATTGTGTTTTAACATTATCAAAAATAGCTAAAGTTTCTAGCTTTTCAATATCATTTTCATTTAATTTTTCATCTAGTATGAATTTTTCGTAAAGTTCTATCAAACAGATGCTTTCGTCAATTTTTTTACCTTTTAATAAGTCGATTAATAAATCAGTTGAAGCTATAAAAAATGCACAACCTTGACCATCAAATTTAATGTCTTCAATTTGATTATTTTTTATATTTAGTTCACATTCTAAATAATCAGCACAGCTTTCTCCGTGCTTAATAATTTTGTTTTTTAAACTTGAACTTTTGTGTCTAGGTTTCAAATAATGTTCCATTATTATTTGACGTTTTTGATCTTTATTATAAGACAAGGAAATCACCTCCTTCTTTTAATGCTTTAACTAATTTATCAATATCTTTTTTGGTGTTATAAATACCCAAAGAAACTCTTACAAAAGACTTTTCTTTGGTGATTTTTTTAAGATAATGATCACAAAATACACCTGCTCTTACATAAATATTTTTTGTTCCTAAATAATGTGAAACATCATGACTATTATAGTTTTTTATATTGAATATTATAATGTGATTTTTAGGATTGCTATATAGTTTTATGCCTTCAATTTTGCTTAATTCTTGATAAGCATAAAAGCTTAATTCGTCTAAAATTTTTTGAGTTTCACAATAACCAATTTGATTAAAAAAGTTTATTGAAACACTAAACATATTAATTGCTGCTAAATTAGGTGTTCCAGCTTCAAAGCGATCAGCTGCTGTTGACAAAGTATATTGTTTTTCACTGTCAATATTGTTAATTATTCCACCGCCAACAAAAGGTGCTTCAAGTTTTTGTAGAATTTCTTTTTTAACTGCTAAAACTCCAAAACCGGTAGGTCCATAAAATTTATTTGAGCTAAAAGCAATTGCATCACAATTATTTAATGAAACACGTTCATAAACTATTGCTTGAGCCGCATCATTAACTACTAAAGTATTAAATTTTTTAGCCTTTTTGTAAATAGCATTTAAATCAATATTGTTTTCAAAACTATTTGAAACTTGACTTACTGCAACAATTTTAACTTTTGAATTTAAGTCATCTAAAACATTTTGACTTATTTTAACACTAGCCCCAATTTCATTAGCTATTTTAATTCAAGGCGCCATATTTGAAGAATGATTATAAACATCTAAAAGGATAATATCCCCTTTTTTAATTTGATTTTTGATCATTAAAGCAAACTTGTTTAGTGAATCAGTTGCTCCTGAAGTAAATATAACTTCTTGTTCTTGACAAGACAACAAAGTAGCAACTTTTTTACGTAAGTTGTTTATTTTTTGCGCGCTTTCAATTCCTAATTTAGTGTTAATAGTACGGGTACTAATTGACTCATTTAAATAAAAATTGTTAACTGCATCAATAGCCATTTTTGGTTTTAAAACTAATGCACTACTATCCAAATAAGTTATCTTTTTTGCTAAAGGGAATTGTTCTTGAATTAATTTGTCTAATGTTTTCATATTTTAAATTATAAAGTTATATTTAATATTTGTATACAAATGTTACATATAAAAAATAACGTTTTTATAAAAAATATATAATTAGTACAACAACTTAAAAAAGGGAAAAATGAAAGAAGTTAAGAATTAAAATAGAGCAATATCTTACTAATGAAAAAGAATTGCTCTTATTTTTTATAATGTTCAATAATTTTTTTGTATTTTTCTATAACTATTTCAATTGCTTCTTCTCATGAATTAAATAAATTGTTCATATTATTTTTCACATTAATTAGATTATTTTTATCTTTAAAAATTTGTTTAATTTTTTCGTACATATTGTCAACATCGTCTTGGCAAATAAAACTATTTTGTCCATCTATCACATTTTCGGCACATGCTGTGTTTTCAACAACTAAGAGAGGCAACTTTAAAGCTGCAGATTCTCTAACAACAAGTCCATAAGTTTCATATAAACTTGGATAGAAAAAAAGATCATGAGATAAATAAATGCCTTTTAATTCTTCTTGATTTTCTATAGGGCCAGTAAACAATATTTTATCTTTTAAACCTATTTTATAGGCATATTTAACAATTTCCTTGTAATTGATATCACCACCAACCATTGTTAAATGGTAATTAGAATTTTCTTCAACAAGTTTTTTATAAGTTAATAGAACTTTTTTAATATTTTTTTCCCAAACCAATCTTGAAACATATAATAAATTTTTATTATTAGTGTTTATATTTCATTTTTTTATAACTTTATTTTTTAATTCATCTGCATTTTCTGGATAAAAGAAATTTGTCCCTAATTGTACAATTTCACTTTCTTTTTTATATCCATATTCAATTAAAGCTTTTTGCGCAACTTTTGACACAGGTCAAATGTAATCAAACTTATTAAGTTTTTTAATCATTAAATGTGTTAAAAATTTTGCAATTGGCTTAGACTTTGTAAAACGCAAAAATGAATCATAAAACAAAGTATGAAAGCTACAAATAGAGGGAATTTTAAATTTTTTTGCAATTTTATTTGCGATATCTCCGGCATAAAAAGGTGTATGAGAATGGACAATATTAGGTTTGAAATTTTCAATTTCTTTTCAAATTTTTCTACTCAAAATAGTGTTCATTACTTCATAACCACCAATTTTAGCTAATTGTCTAGGTATTCTAATGACAGAATAATTAAGACCTTTATCTTCATCTAATTTCATTTTTTTGTATTCTGGAGCCAAAACCAGAACTTTAACATTTTTTTTAATAAAATTAGAAGCATAATTTTGCATTACCCTGACAACTCCATCTATTACAGGAGTAAAACTATCATTAATTAAAATAACTCTAATTTCACTATTTGTCATTTTTCTTTGCCCTTTCTTTTTTACAAAAAACTTTCATATCATACATTCCTAAATGTTCAGACATTTTGTATTTTTTTGGAATTCTTCAATTTGGACCATAGTTTTGTAAAAATATTGTTTCATAATTTTTTGGCACCCGAATTTTTATATCTTCAAATTTAACATCAATAGTATCAAAAAAATTGATATTTTCATATATGTTAATTTTTGTAGCCTTTTTAAAAGGCCAATTAATTACATGATGAATATTTGAATTTGGTTCATACAAAGTCTCAAGAGCTTTTTGATAATTTGGTTTTTTAAACATTCTTGTTCAAAATAATAAAACTTTAGCAATTTTCATTCCTCATTGAATATCAAAAAACTTTCTTTGAGAATAAGCGTTCAAATAACCCAATTTATTTTTAAGAGATAAATATTTTTTTATTCTTTTTTTAGTTGTTGGTATATCGATAAAAAGATCTAAAAAAGTTACATCTTTTTGAGAATCTTTATCTTTTGAAAATTTAGGGAACATTAAAAAGCTGTTGCCTGTTTCTCAGTCTACAATTAAATTTCTGTAATTTTCCTTTAAAAATTTATATGTTTCAATTGAAATTAATAAATCAATATCATCATCTCAAGGTATAAATCCATTGTGTCTTATTGCTCCTAATGCTGTTCCATACATAGCACAATAATCTAAATTGTGTTTATTACAAATTTCAATAAACTCTTTCAACAGTTCATAAACTTTAGTTTGTTTTTCATTCATAGTTTTTATTATACTTTAAAAATTTTTTGGCAATAATAAAAACTATTATCTTGAAGATAATAGTCTTATTAATTATTTAAATTGCATTTTTGCATTGATCAGTTTCGATATATTCTTTTAGATTGTCATAAGTGTATTCAACCATGTTTTCAACAGCTTCATCAGTATAAGAACCAATGTGAGGTGTTAAAACAAAACGAGGATAGAATTCTAATAATTGGCGAAATGTTTTGTTTTCTATTTCTGCATGCTTTTTAAAGAAAATTGATTTTTCATTGTTCAAAACATCTGTAGCAATAGCTTGAATTTTGCCACTTTTTAGACCTTTTAATAATGCTTCTTCATCTTGAATTTGGCCTCTAGCTGTGTTAACAATAATTACACCTTTTTTCATTTGCTTAATTAATGCATCATTAATCATTTTGTCGTTTTTACCTTTAATATAGGGGCAATGGAAAGAAATGATATCTGATTTTTTAACTAATTCTTCTAGAGTTTTTGTATATTTAACAACTTTTTTGCCTTCTTCTGAAGGATATAAATCGAAGCCTAAAACATTGTTTGTCATAACTTTAAACATTTTAGCAGCTGCAAGTCCTATTTTTCCAACCCCAACTATTCCAACTGTTTTATTTTTTAATTCAGTAGCAAAACCTTCAGGATCAATAGTAAAGTCTTTGTTAAAACCTTTATGTCCAAAATGAAGTGTTTGTCTTGATAACATTAAAGCATGAGCAAAAGCTAATTCAGCAATTGCTGTTGGTGAATATGAAGGAACTCTAGCCATTTTAAAGCCTAATTTATGAGCATATTCTAAATCAATATGGTCTGTTCCGACAGTTCTTGTTAAAAGATATTTAATGCCATTTGCTTTTAATGCATCTAACATTTCTTTATTTATTGAACCATCAACTCTAACAATAACAGCGTCAAAACCTTTTGTTAAATTGACATTTTCCATAGTTAAACTTTCACTATGAATTTCAAGTTTATAACCAAATTTTTTGTTAATTTCTTCAAATATTGGTTTTTCAACATTTCTAACTTTAAAAGTAATAATTTTCATAATTTTTCCTACATAAATACTAATTCTGCAACTACAATTCAAATAGGTAAAAAGATAACTGCAAGTAATGTACTAAATGCTGAAGCAGAGCTTGCTAATCTAGCACATTTATTTTCACTAATTGCAAAACCAACAGCAACTGTAGCAGGTGGTGTAGCTGCAAAAATAACCATTGCAATTCCTACTTCTTTTTTAACTAATCCACCTTTGTTTAAACCTCAGAAGATTAAGAAGATTGCAAGTGGAATAACAATCAATTTAATTAATGAATATATTCAAACTCATTTATCTCCTGCCGCTTCTTTTAATTTACTTTCAGCAAGTGTCATACCAATAGCTAATCAAATAAGTGGTGAACATAAACTACCTAAAATATCAATAAATTTATAAATATATGGTAATGTTGTTTTTCATGTAAATCAACCAACTGGTTTAGTGGTTTTAATATATTCATTATTTGCATTTAACATATAAAATACTTTTTTGTCTGGTGTTAAAACTGTTTTAAAGTTTTCACCAAAAGTAGCTGAATATTTAATAACTACATCACTTCCATTAACTTTAATAGTTTGACTAAAATCTTTATATGTTGCTCCTGCACCTGGTATTAATTGATTCAATCAAAGAATTAATCCAGCAAAAGTTGCAATAATAATTGGGTTTAAAAACATTGATTTTGCTGAAGCTTTAATATTTTCTTTGTTGAATTTTAAACCTTTCATTTGCATGAAGCAGAATGAATATAAGAAAATTCTATAAGGAACATTTCAAATATTTGCTGCTAGAACACCATTATTATTTGAATATAGTTCCTTTATAATAGGCATACCAAAGAATGTTGTTGATCCGAAGATTAATAACATTCAAACAACCATTGCGCGATGACGAGATTCATCAACTGTTTTTGTTTCACTCATTTCACCAATAATAGTGTTTTCTTGAGCATTTTCCACTGATTCTGGTGTTTTATGAGGCATATATTTAGTTCATAATCAAGCCACTAATGAAAGCAAGATATAGAATCCAAATGCAATTCCTAAGATAATACCTTGTTCTTGAAGAAGTCCAAGACTAATATCGCTCATAAAGCCTTTGAGTGCTAAAGCTGGTAAAGCAACTTTCATAACTACGCCAACAAGTGCAGCTTTTCAAGTTGATTTAAATCATTTAACTTTAACCAACAAATAACCTAGTCCGATTATTACAATAGAAGCAATAATTGCGCCTCAAAGATTTACATTAGTTAAAGTTGAAACTAATTTCTTTGTAACATCCATTTTTCTCCTTTAATAATTTGCTCATAAATATTATATTTTTTATATTATAAAAAATGAAATAAATAAAAAGGTTTTGTGGAAATTTTCTAGAAATTGGAATTTTTATAATTGTGAAAATTACTATAAAACAATGGTTTTGGCAAAAAAGCTAATAAAAATACTTTACACTTTACTAAATAAATTTAGCAGTATAAAGTATTTGAATTAATTATTTTGTTCTTGGTTTTGATGACTATTATTATCATTTGTTTCCTCTGCTTTTAATTCTTTCTTTTTCAATTTCCTAAAGATAAATTTATCATCAGGTATTAAAGTTATTATTTGGCTTATTAGAATTAAAGAAGCTAATAAATAGCAATATCAATCATAAAATTCAAGTCTTATCAAATCAAACAATATTGTTCAACCAACATAGGAAATATTTAATCCTGTAGCCATTGGAACACCTATTTTATCAAGTGCATAATAGAATGTCATATATGAAATGCAACCCACAATTCCAGAAGCAACAATTAATAAAATGTTTGTATTAGCAAATAGTTCATCCATTTTTTCATAGATTTTTGCTGATGGTAAAACAATGCTACCTAAAACAACCAATGAAACAACATATCTAAAGAATATAGAAACAAAAGGATCAATAGCATTTTTCATTGATAAGGTTGATAAAAATGCTTCCAAAGCTCAACAAAGTGCAGCTAATATTGCAAACAAGAAACCTCATCCATTTTTAATATCGCCTTTATGAAATTGTAAAATTCCTAAAGTTAAAATACATAAAAGCGAAATTATTGATCCAAATAAACCATTTTTTGTAGTTTTTTGTTTCAAAAAGATAAAAGCAAGCATTGCGCCAACAATAGGATAACAAACTGTAATTGCACTACTTATTCCAGTACCGACATAAACAATACCAAGGACATACATTGTCATACCCATTGGTGCCCCAAAAAGAGAGCTGAGTAATAATATTCATATTTTTTTATTTTTGAAAGCTGCAACAAATTCTTTATGTTTTTTAAATGAAAAAATAATTATTATGCTAACTAAAAAGCCAAATGTATCATTTAAAAAAGTTATAGCTAAAGCTAATTTAGCTGGGCCTCATTCTCCTTGATTAAAAAATGAACCGGAAGGAATTAAATTTGTAAAAACAGAAATTAAAATTCCATTTAATGCTCACATAATCCCTGAAAATAAAGCAACAAATTTATTAAATAAATTTTTATTTTTAACTTTTTTATCGTTTTGAATTTCATTAACTTCTACATTTGAATTTTTATCATTCATATTTCACCTTGTTTACAAATTTTAATGTTGCAGAATAGTAAATTTTGTAATAATCTCTGAAGAATTCATTAAATATTTCTTTATATTCGCACCAATATGTTCATAAGAAACCTATTGAAGCAATTAAAATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGACATAAAACTTTTGGATAATTTTTACTTTTATGATAAGCAAGCATTTTTCTAATTTTAGCTTCATTGTCTAATAAATGTTTTGGAAATCTTTTGTATTTTTCTCCAATTATTGAAATGTAATAGTCCAAAATTTTTAGAATATCAAATTCAAAATCAACTGATAAATTTTGATTATGTAGTTTGCGATAACTATTTAAAGCTAATTCCATTTCTTTATAATTGTGTCCATCAATTGTTTTTGAGTTATTAATTCATTCAGTTATTTTAAAACCATGTAAAGGATCATCAATATTATTTTTGGTTTCAAAATAAATTAAATTTTCAACTAATTCATTGCCTTTTAGTTTTTGATAAATTTTTTCTTCTTGGTATCTATTAATTAAACTCTTTGAGCCATCTTTTGGTATTCTAGCAATATATTTTTTATCTTTTATTTTAAAAGAAAATGAGTTATTAGTCATTCCTTTTTTAACTGGTTGAATATCTAAAATATCTTCAAATTTGGCATTAAAAACTTCTTTAATTTTGTTTAAATAAACATCGTTTATAAAGCAAGGAGCTTTTCTATTAAATGTCAATAAATCTTTGAAATCGTCTATTTCATAGACCTTTTGGTCACAATTGTATTTCTTAAGCTCTATATCTTTTAAGATATCCATCAAAGCAAATTCTCAATAATCATTTTGCTTTGAAATTGGATTTTCAACTCTCTTAATAATTGCATTTTTAAATTTCAAGAAATCTTTTTCATGAATAAATGAAGCACCTGTAATAAATTCAAAATTATCACTTTCATAGATTTTATTTAAGTCTCTAATTCTAAAGTCAACTATGTTTTTATTCTTTTCAATAATTTCTCATTCAGCTTTATTTGTTGTACTACTCAAAGTATTAACCCATGAGTAATTAAATTCCTTATTAAAGATATCATCTAAAAATAAAGTGTCATTGGTTGTAATTAATAAACCACCCTTAATTGTTTTCAAATCTTTAATTGCTAGATATAAACTAAATAATGAATTAGTATCTGTGAATTCTTTGTTGAATATCTTTTTGATATTTTCTTTTTTAATTAAGTCATTTCAAGATTGATAATTGTAGCCAGTTACTATTGTCTTATTTTTATAATCTTTTAAATAAGATAAAAGATGAGGAACAATTTTTAAATCTTTTTTAATTTCTAATAATCCTTTTGGCGTCTTAAGTGAAAGAGGCGTTAGTCTTGAACCAAAGCCAGCAGCTAAAATTATTATTTGAGTATTTTTGTCCATTTTTCTCCTAATTTTGTTATAAATTATATAATCAATGATATTATAACCATATATAAAAACTATAAATTAAGCAAATGTTCAAAGATGCTGAAAAAAATTTTGTATTTTATTAAATATTTGTTTCTCAAAAAATAAATTTAATATATAATAAAAAAGCAATTTATAAATTGAATTAACGTTTAAGGAGGAAGTCATGAGACAAACCACTATTGTAAACAAAGAAAAAGCTGACAAGAAGTGATATGTTGTTGATGCTGAAGGACAAGTTTTAGGTCGTTTAGCCGCTTTTGTAGCTTCTGTTTTAAGAGGTAAAACAAAACCTACATACACACCAAATGCAGATATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAATATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAATTTCACTTTTTTATAATAATATTAAGTTTTGCTCAAATTTTGAGTGAAACTAAAATGCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCATTTCATTAAATTGACCAAAACCCTTGTTTTATAGGGTTTTTTATTTTTTATTTTTTCTCAAAATTTTAATTGCTATATAATATAAAATTAATATGATGATTGATTTATGATGTCGAAATTTTGAGAATCAATTGTTTTAAAAAGGAAAAAAATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGATACTCACTTGCTTATGGTACTCTTTTAGGAGCTACAAGAAATAAAAAACAAATTGCATGAGATACTGATATTGATGTTCTAATTACAGAAGAAACATATGAATTCTTAAAAAAACATTATCCACAATATATTTTGGATAATACAACATCGCATCACTTTCTTAATTTTCCACGTTTTGTTGATAAGATTGATCAAAATGATCCTAACAATATTTATATCGATATGTTCATTATTGTTCCTTCAACATGGAAGAAAGCTAAAAAAATTAATAACTTTTGATACAAATTAAGATCAATGAGAAGAGCTTTAGCTCCAAGAAAATGATTTATTAATATTTTTGGAAATATTTTAATGTTTATTTTTGGCTGAATGTTTTTATTTTTAGTACCACCTTTAGATATTAAAACAACTATTGAAAAAACTAAGGTTTCAGAAAAAGAAAAAGAAATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGATATGGAAAGACATGAAGAATACCAATTCAAACTAAACATCCTATTTATTATGGTTATTATGAATTTAAAAAAGTTCCAAAAAAATATCGAGATTATTACAAAAATGAAAATGCAGGCCAATAACCTGCTTTTTTAATAAAAAAAACTCAAATTAATGAGTCCTTTTTTGGTCTAAAGTGATATGGTGCCCAAGACAGGACTTGAACCTGCACGGATCTCTCCAGTGGATTTTGAGTCCACAGCGTCTACCATTCCACCACTTGGGCAATTATAAATTATTATAAATGAAGTTTTATTTTTAGTAAATAATTTTTCTAATAAAGCAAAAAAAGTATAAAAAAATAGCAGCTCCCTATTTTCCCATCTCTGGTATCGTCGGCACTAAAGGGCTTAACTACTGAGTTCGGAATGGAATCAGGTGGGTCCCCTTCGCTATAACCACTGGATATATTATAACATGCAATTTAAAAAATCAAGCAAAAAAATAAAAAATTTTTTTCAATTTTTTGAAGTAATTTTTTTAGTCAAAAAACTGAGTAAAAAATATCTTTGTTTAATATTAATTTTTATATAATTGTAATGTATTTATTAAGGAAGAGATGAGAAAAAATAATAAACAAAATAATAAAGAAAAAGAAAGCAAATTTAAAAAATTTTGAGAAGAATGAACAATCTTTCCAAAGTGAACTATTAGAAAAATTTCTTTTGTTGGAATCTTAATTGCAATTTCGGTTGTATTTTTAGTTATTGCTTCTTCTCTTGTTCCTATTGCTTCAATTCCAACTTATAAAATAAGTTTTATAGGTTTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGAAAATTAAGCAAAAAACTTGATAAACTAAAATTAAAAAATACTAATGATAAAAAAATACTTAAACTAGAAAACAAAATTATTCTAATTCAAGAAAAAAGAAAAAGTATTCGGATCAATGGAACACAAAATATTTTGCTAAATATTAATTTATTTGTTGCTGTTATTCTTTTAGCTTTAATATCATTATTTATTGTCTGATTAGTTGGCTTTGTAATTCCTGAAAGCATTATTAAAAAAGGTGTTATTTCGAATAGATGAGGGCTTTTAGCAACAATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGATTTAACATGTTTATTATTTATTTTACATACAATATTATTGCTCCTCTTATTTATAAAAATAAAAGTATATCTTATGATAGTTAATAAGTGAACCTCAAATTTCAAACTTTTTGGAAAGGGGTTCATTTTTACGCAATTAAAATTTAAAAAAATAAAAAGGAAGAAAATCTAAAATAAATTTTGATGCTCAAAAGGCAACAAATTTTAAAATTAATATTGAAAAAGTAAATTTTTTATGAGATATTAAAATCACAAAATAAACCTGACAAAGATGAAAATTTGAAAAAGATTATATTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCATTAAGAAATAAAGGAACAATGATTAATCATAAAAAGGTTTAAAAATTAATAAAATCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATCCAAAAGGATGTTCACAAAAAACATTCCAAACTTGTTTTTAGATAAAGAAAATATCGCAAAATATTTCTTAAGATACAAAATTTTTTAGTAATAAATATTTGAAAATACTAGGAACAGATGTTATTGAATTCAAATTAAAAAAATGATAAAAAAGCATATTTTCTTCTGTGGTTGATTTTGAAAATAGTGAGATTTTAGGTTATTCGATTTCTAAATCACCTAACTTAAGAATGGGGGGTAAAATGTTGGAAAACGTAGAAGGGAATGAGCATAGCTTAAACAATGTATTATTACATTTTGATCAAGGATGACAATATACTTATGAAGATTATATAAATTATTTGAAAAAGAAACAAACAATTCAAAGCTTGTTAAGAAAAGGAATCGTTTAGACAATAGTCCCGCTGAATGTTTATTTAGTGTTGCAAAAAGAGAATTTTAATTTTGAGAAAAAATAGATTTAAAAGTTTTAAAGAACTTAAAACTACTTTAGATTATAATAATGACAGAATTGCTAATAAATTAAAAGGACTTAGCCATATTCAATACATGAATAAGTCCAAACATAATTAAATAGTTCAATAATTGAGGTTCAAACTATAATGATTGTCTGTGCAATTATTTTTTTATATGTTGATTTTTTATATGCTTTTTAGAAAAAGTCACAAAAAATATTAAATTCTAAAAACATTACCATTAAATATTATTTATATAAATAAATTAATATTAATTTATAATATTTTAAAATATAATTACTTTATAAGTTTTAAAGGAGTCTGGAGGTTGTATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGAGTAGCAGAAGCTGAAAATAAAAATTTAGATTTCAAAGATCTAGAAACAAATAAAAAAGGCGAATTATATCGTTTATTAACAGTTGATTCACGTTTCGTTCATAAAGGAAATAATGAATGAACAATTAGAAATGGTTTTGAAATTAATAAATAATATTTTGAAAATAGGAGTACAAAATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGAGCTAAAGCAGTTTTATTAACAGCTCAAGAAGAAAATTCACCTGTTATTGTTGCAACAAGTGAAGGCGCAGTTAAATACATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGAAAATCATTAAACTTCGACAAATTAGTTGAAATTAGAGATGCAGCTAAAATTGGTATAGTTTTACATGGCGGTTCTGGTATTCCGGCTGATCAAATTGCTAAAGCAATTAGTTTAGGTGTTACAAAAATTAATGTTAACACCGAATTACAACAAGCAAATCACAAAGCTTTAAGAGCTTATATTGAAGCAGGAGAAGATCTTAAAGGCAAAAACTTTGACCCACGTAAACTTTATGCACCAGGTTATAAAGCTATGTGTGAAACAGTTAGAGCAAAAATTAGAGAATTTGGTTCAAATAACAAAGCTTAGTTAAAATTTAAGAGGAGTTAAAAGCCTCTTTTTTATTTTTCCATTTTTTTAAATTTATTTATAAAAATGACTAGGTATAAATATGAAGAGATATTTGAAACTTTTTTTAAATTTTTTGCCTTTTACTTGTTTATCTGTTGCTGGTTGTCGACAACAGATTATTAATCCACAATTAAATCAAGTTTTTGATGTCAAATGACATTATCAAGTAGTAGATGGTGACACGTTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGAATTTGCAAAGTTTATAGTGCAGAAGGTAATGATTTAGCTTTTGAATTAGTTAAACAAGGATTTGCTAGAGTATCTTATATTTCAATTGATTATCATGCTAAATATTATTGAGTTAATAATAAAGAACAAAAAGAATATTTTTGAAAATTAAACCAGTTGCAAAATGAAGCACAACAAAATAAAAGAGGATTTTGAAAAGAAAAAAATATTAATTTAGTTTTTAATAAGAATTAAAAAAATACGCAAAATGCGTATCTTCTTGTTTGCTTATTAAGCAACAAATTCAGTAATACTTTCGTTTTCAACTACTAAAAAGCGGTCGCCTTTTTTAACACCATATGGTTTAAGAGCATTAAGAGCTTTGCTTTTATCTTTTTTGATTTTTGCAAATAATTCGATTGAATCTAATGATACTCAAACACTAGAAGTAACACCAAATCCACCGATTAGGTTTTCATCATTTAAAATACCAACGATTAATGTATTTGGTCTAAATTTAGCAACTTCACTTAATAATTGACCTGTTCTTGAAAGAACAACAGCAAATCTATAGTCACCATAACGTGTTCTGTTTGCAACGTCATAAGCAATTCTTGAACGTTTTGAATTTGAAGAATTTTTTCTAATTTCTTCTAATTGAACATCATAGTAATTTTTGTCATAAAATTCACTTTCAGCACGTTTGTTAATTTTTGACATAACTGTAACTGCTTCTAAAGGATAGTTGCCTGATGCACTTTCACCTGAAAGCATTGTTGAGTCAGCACCCATTTCTGTTGCATAGTAAACGTCAGTAACTTCAGCACGTGTAGGTTGTGGTGAGTTTTCCATTGAGTCTAACATTTGAGTTGCAACAACAACTGGTTTACCCGCTTCACGACATTTTCTGATCATTATTTTTTCAAAATAAGGAACATCATAGTAAGGAATTTCAAGACCTAAGTCCCCACGAGCAACCATAACACCATCTGATGCTTGAATGATTTCATCAATGTTTTTGGTTCCTAAGTGGCTTTCAATTTTAGAAATGATTTTAACGTGTTTTCCACCATTGTCATCTAATAATTTACGTAATTCTTGGACATTTTTAGATGAGTTAACAAATGATGCTGCTACATAGTTAATTCCGTTTTTAATACCAAATTTAACATCATCAATGTCTTTTTGAGCTAAAAATGGAAGAGAAAAGTCAACACCTGGTAAATTAATACGTTTGTTAGTTTTTAATAAGTGCTTGTTAAGTGTTTTAACAACAACATAACCAGCTTTTCTATCAACTTTAGTTACAATTGTTGATAATTTACCATCGTCAAAGAGAACTTGGTTTCCAACAACTAAGTCTTGAGCCATGTCATAAGCAACAGTTAACTCTTTTTCTGTTCCTTCTACGGTTTCATATTTTTCTTTAGTTGTTCAAACTTTAATTTCTTTGTTTGCTGGAATTTCTTGTTTTCCATCTTTCATTTTTCCTAAACGAATTTCCGGACCCTTAGTATCTAACATAAGAGATATAGGAAGATGTAAATCTTTAGAAATTTTTTGTGCAAGAACAAATTTGTTTAATTGTTCTGCATGGTCTCCGTGGCTAAAGTTAGCACGAACACAGGTTGCACCTGCTTCAATTAATGAACGAAGCATTTTGTAGTTATCACTTGATGGACCAACTGTAATAACTAACTTACTTCTTTTGTCAGTAATGTTCATTTATTCTCCTTAAATTAAAATAAACTTGTATGTATATATTTGTAATACTAAATATTATTTTATATTAAATTTGCAAAATAAATAACATATTTTTTTATTTGAACCATGATTAATAGTAAATTTGTAGTTTCATTATTTTCTTATTCTTTTTTATTAATAAAAAAAGAGCAATTTGCTCTCGATTTATGATTTTTTAAAACCACCTAAAGTTATATGTCCAATATATTCAGGAATTGAAGAAGACTTATAATTATTATTTGAATTTTTAACTCTAATACCAATTGCAAAGGTAGCTTCGCCTTTTGCTGGATCAAATGAAACTAAACCTTCATTTTTATATTTTGTTTTGTCTTTATCATAATCTTCTGTTTGTTTTGTACAATAGAATAATTGGTAAAGGTGACTATTTTTAAAATCATCTTTAAATTTCAATTGAATTCTTTTGTAGTAACCTTTACTTGCATTTTTGTATTCACCGAATTCATCTGTTGAATTATTTATTCATTCAAGTGAAGTTGAAGGTGTTTTAAAGTCAATATTTTTAATTTCATCGTTACCTAAAATAGATATTTCAACATGATTTTTCAATACTTTTTCAAGTTCAAAATAATTAATATCTATTTCTTGAACAATAGGATTTTTTATGTCAATCATTCTTGATTTTATTAAAGATGTTTTATCATTTTTGTCCTTAGGCATTTCCGCTTCCGGCACTATTTTATAGGCATAAAAGTGTAAATTTAATGATTTTTCAGAAGCTTTTGTAAATCACATTCCACGCTTTGTGTCTAATTTATATTCGGGGTTATTGCTTTTTTTATCTAAAGTTCTTCCAATGTCAAATCAACTTTCAATTGTTGAATTATTTTTTAAAATTTCTTCTTTATTTTTGTTTAAATCACTTGAAAGATCTTTTTGTGGTAATGCAGCAAGTAATTCATCAAGACTACTCTTTTCTTTCTTGTAAAAAGTTTGTCTTATTTTATCTCTAATATCCATTTTTCAAGATTTAGGATTACTAATTAATTTTGTGTTCGTTTCAACGACGTCTTTTTCAGTAACTTTTTTAAATCCATTTAAAACAAAAGTTTTTGATGTTCAGTTTTTTATTCTATCACTAATGTTTCTTGTTTTTTTATCTTTATCTTTTTCATTAGCTTTATCTTCTAATGAAACAGTCAAATAGAGTGTACCTGTAAAATCATTTGCATATGAATGGTAGAAAATATTGTAATCTTTATAAAATTTGTCAATTCTATCAAAACGATTATTTCTTAATGAAAATAATTGATTTCTCAATGCCCCTTGAGGAGAAAGATCATCATTTTTTAATCAACCTTTTTCAATATTTCCTTCAAGTTGAGTTCTTCAATAATTTTTTTCATATTCTTTTGTGTTTTTCAAAGTTGGTTCTTTGTTGCTATTGTATGTTTCAAAATAGTAATAATTTGAGTAAGTAAATTCACTTGCTAAGTGATTTGCAATATTAGTTTGGTATAATTTGTCTTTAGGTTTTGCAAGAGAAAAAGTAAAAGAGCTTAGCTGTCTTTCAATACTTTTATTGTCTTTATTAGTTTTAACTAAAGCATAAATAGCCGTACCAATTCCAACTGCAACAACACCTGTTGCTAATGTTCCTAATGTTAATGATAATTTACGATTCATTTTTTTCTCCTATTCAAATATAATTTTTAAATTATATATCAAATATTTGATATTAATGATTTTTACTCATTATAAAGTAATTTTTCTATGTGTTGAACTATATCATCAAGGTTAATATTTTTTTGGCTTGATACCAAGAACACTTGAGCTGATTTTTTAAAAATTTTTGAATTTAATTCATTATGTTTTTTAATTAAAGCCGATTTTTCTTTTTGATTTAATTTATCAATTTTTGTATAAACTATGTCATAATTTCAGTTTAATGAATTTAAAAATGTCAAGGTTTCTAAATCATTTTTAGTAAATCTTGTTCGACTATCTATAAGCAAAAAGATATGTTTTGGAGAAGGTTCATTTTTTAAAAAATCTTTAACCGCAAGATTCATTTTAATGACTTTTTCTTTAGATATTTCAGCATAACCATAACCATGTAAGTCAACTATATATTTATCATTGTTATCTGCAAAATAATTAATTAATTGTGTTCTGCCAGGGTGCTTTGATACAAATGAAATATTTTGATTTGTTATAGCATTTAAAAGTGAACTTTTGCCAACATTTGAGCGACCTCAAAAACAAACTTGAAAGCTGTTTTTGCAATCAAGTCATTGTTTTTTATCTATTGCTGAAGTTATAAATTTTCACATTATTTTTTACCTAATTTTTGTTTATGTCTTAAAAGAGTTGAAGAATAGTCTATGTTTTCATAGTCTGGTACTATAAGTATAGTTTCAAGCTCAGAATTTAAGTGATGATTAGCAGCTGCTAGTACTAGCTCATAGTCATAATCTAAATTATTTCTGGCTGATCTAACTAGGAACTTTACATTTTTTTCTTTAGCAATTTGAGCTATCAAATCATTTTCATTTTTTACCACAGTGACTGTTTTATAGTCTTTTAAGGTTTTTTTGACTGATTGATATCTTTCTTCTAAATTACCTAAATTGTTTTTATCAGGGTTAATTGAAACTATTACTATAAGCTCATCAAAAAGCTTTAGTGCTTTTTCAATTACTGATATATGACCTTTGTGTATAGGATCAAAAGATCCTGGAAAAATTGCTTTTTTAATCATAATTATTTATACAAATTTATTGCATGTTTTGCAATTAATAATTCTTCATTTGTTCTAATTACATATACTTTGATTTCGCTATCTTTTGTAGAAATTAAAGCATAATCTTCAATTTTAGAATTATTCTTTTTGTCATCTAATTTAATCTTTGCAAAATGTAGTTTATCAACTACCATTTGACGCATTGAAGGAGTGTTTTCACCAACACCAGCAGTGAAAACAATAGCATCTAATTTGTTGCCAATTTTGTTTGCATAATTAGCGGTAAAATCAGCTATTTTTTGTGCATACAATTCAAGTGTGAAAATAGCACGTTTATTGCCTTCGTCTGCTGCTTTACATACATCACGCATATCGCTTGAAACACCTGAAACTCCTAAAAGCCCACTTTTCTTGTTAAGAGCTTCTGTGATTTGGTGAATATCTAAACCAAGTTGTTCATTAATAAATTCATGAATTGAAGGATCAATATCGCCTGAACGCGATCCCATCATAATTCCTGCTAAAGGTGTAAGTCCCATTGAAGTGTCAAATGATTTTCTATCTTTAATAGCACATAATGAAGCACCATTTCCAATATGTAAGTTAACTAAATTAACTTTTTCTTTTTTAACTAATTCTTGGATTTTGAGTGTAATAAATTCATGACTAATTCCATGAGCTCCATAACGACGAATTTTTAAATCTTCTGTTCATTGATATGGAATTGGATAAATTGCATTGACATCTGGAATTGTTGTGTGGAATGCTGTATCAAAGTCAACACTCATTTTGGCATGTGGCATAACTTTTTTAAATGCTTTCATGGCTTGAACTGCACCTGGATTGTGAAGTGGTGCATAAATTTTAGCTTTTTCAATTAAATCAATTTCTTTAGTATTCAATTTAACTGTTGTTGTAAAGTAAGGTCCACCATGAACAACTCTAAAGCCAATTAATTCGATTTCTTTTGGATTGTCAATTAAACCAATTTGTGACATTAATTCTAAAATGTTTTCAACAGCCACAGTGTGGTTAGGCATTGTTACATCACGGTTAAATTTTTGACCGTTATATTTGATTGTTATTTTACCTTCTAATAAGGTAATCCTCTCTGCAATACCACTTGCAATAATTTCTAAAGAAGCTTTATCAAAAAGTGATAACTTTATTGAACTACTTCCTGCATTAATAACTAATACTTTTTGCTTACTCATAATATTCTCCTATTTCTCTGCCTGCAATGCAGTGATTAAAATTGTATTAATTACATCTTTTACACTTGAACCTCTTGAAAGATCATTGACTGGTTTTTTAGTACCAACAACAATAGGTCCAATTGCCCCATAATTTCCTAATCTTTGAGCTAATTTATAGCCTATATTTCCAGCATCTAAATCAGGGAAAATTAAAGTATTTGCTGGTTCTTTATAGTAATCTAATTTATATTTAGCTTTTCTAACTTCTAAATCTAAAGCAGCATCTAATTGAATTTCTCCAATAGCTTTAGTACCTTGATAAATGGTATTGAATTCTTCAACAGCATTGTGTACTAATTCACTTTCAGGTGTTTTTGCTGAATAGTTAGTTGAGAAACTTAAGAAGGCTATTTTAGGTTCTATCCCCATGCTTTGAGCAAATTGAGCAGCATTAAGTCCAATTTCTACTAAAGCTTTTTGATCAGGCTTAACATTTACTGAAATGTCACTGAAGAAGTAAGTATTTTTTTCGTTATGTACTACCATAACTGAAGAAATAGTTTTTACTCCTTCTCTAGCGCCTATAGCTTTAAAAGCTGCTCTTAAGATATCTGCAGTTGCATAATTAAGTCCGCCAACTACCCCATCAACTTCACCTGTAGCTAAAAGCATCATAGCATAGAAAGGTCTTGTTTTTAGGTTTCTTTGAGCTTGTTCAATTGTTTCTTTGCCTTTTCTTAATTCAAAATATTTTTGAGCTAAATCATTTTGTTTTTGTTTATCTTTATTAATGTTTATAAACTTAACATTGGTTTTTGACTTAACATCAGTTTCAACTAATAAAGTTAATTCAACATTAGGAAATTGTTCTAATTGTTTAATGGTATCTAGTGCTCTACTATCATCACCATCTATAAAAGCAATTCTTTTTTTAGTTTTCATTGCTTTAATTCTTTTTTCAATTAAAGATGTAAATTTCATGAATACCTCCTATAAAAAAATATTCTTATATTAATTTTATATATATTATTAAATTTAATAATTAAATAATTACAATATGGCAATTTTTATCTTAAAAGGTAAATAAAAAGAACCTTAATTAAGGCCATAAACCAGGACAAAAGAATTAGACTTAAATCTCAAATATATTTAAGTCTTTTTGTACTAGTTTAAAGCATTAAATGAGGTTTTATGGGAAAACATTTAAATATTGAAAAATAGATAGAAATTTTTATATTTTTTAAGGAATATTTAGACTATAAAATTACAAAGACGGAATTTAAAAATGAATATTTTAGAATCAGTCAAAGACAAATATTTGATCGAAAAACAATTAGAAGTATCAAAAACAAATTTAAAAAATACAATTTGGGCATGAGTATAGAATCAAACACAGGAAAATCTCCTAAAAAAGGTAAAGGGTCTGGAAGACCTAGAAAAAATGGATATTGAAGCTGAAAAAAAGAATAGAGAAGAAGAATTAAGAAAGATGACAAGAGATCAACTTGAAATCTTAATAACTGATATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCAATTAAAACAAAGAAAAAAAGATTAAATATGAATATGAAATTAGACAAGCTTTTAAAGATTGTAAAGGTAGATATGGAAGAGCAAAAAGAAAAAGAGAAAATAAGAATACAAATGTTAAATATGAAAATCTATCTGAAAGAGATTATGATGGAAAACATAATGATATATTTGCTACTGTTGTAACATACATTCCATCTCCAATTGATGTTTTAGAAAATCATGTTTATTTATCTGCAATTATTCATCATAGAATTAAAAAAATAGTTAGTTGAAATTTATCAAAAAACAATGATAATAATCTCGTTTTAGAACATTTAAAAACAACTGATTTTTCTTCAAATTTTATTATTCATTCAGATCATGGCTATCAATATTCATCAAATGATTATGTAAAATTCATATCATCAAAAAATGAAAAAATCTCAATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGATATAATAATGATAGATTTATGAAAAAATTAGATCTAAAAACACCCCAACAAATGTGGGATGTTTATAAGAATAATAAATTTATTGGAGTCTAATTTTTTTGTCCTAGTTTAGTGACTTTAGTTTTTTAAACCCAAAAAGTTTGTTGAAAGCCCAAATTTTATGGTTTTTAAATTTATAAAATTATAATCTGTGAATATGTATTATTGATTTAGCTAAATTAGTAAAAAGTGAAATAACTGTCATTGGACCAACGCCACCTGGAACAGGGGCTAAAGCACTTAAATAGTTTTCAGGTATTGTTTCAATATCAACATCACCATGAAGAATTTTTTTGCCATCTACTTCAATTCAGTTTGATCCAACATCAACTACAACAGCACCTTCTTTAATGTTTTCTTTTTTAAGAAGTTTGGCTTCACCAGCAGCAACTACAATGATATCAGCATTTTCAACACCTTTAATGCCTGTGTTTCTATTGTAGGTAGAAACATTGCAACCTTCTTGTTTTAATAGAAATGCTGTAGGTTTTCCAACTAAACTTGAACGACCAATAACAGCAGCTTTTTTGTCAATCATTTTGATATTGTAGCTATTTAATAAATCTAAAATACCAATAGCAGTAGCTGGAACTAAGGTAAAGTCATCCGCTTGATTGTAGAAATTGAATGCATTTCTGGTACATAAACCATCAATATCCTTTTCAATGCGAACGCTATCTAAAATAACTTGTGTGGCAAATTCTTTAGGTAAAGGAAGTTGAATAACAATACCATCAGATTTTTCATTAATAATGTCTAATTTCTTAAGCAAATCTTTTTGTCTAATATCAGCTGGAAAATGATGATGAATTGCTTCTACTCCTAATTCTTCAGCCTTCTTTAATTTGTTTTTGATGTAGTGAGTAGATGCGGGATTATCGCCAACTTGAATAATTGATAAAATTGGTTTTCTGCCTAACTTTTCCTTTATATCCTTAAATTGTGCAATGAGTTCTTTAGTTCTTTTTTGAGCAAGAGTTTTTCCATCTAAGATTTTCATTTTAACTCCTCCTTGTATTTGTCTAATTCTTCTTGATATTTAGCTAGTTTTTGTTTTTCTTCTTCAACTTTAGATGTAGGTGCTTTAGCTATAAAGTTTTGATTACTTAAAATATTGTTTGAACGTTTAACTTCGCTTTCTAAATTAGCTATTTTCTCTTTTAATTTAGCAATGTAAGTGTTTTTTTGCTCGGCTGATTCTTTAATATAAACATTAATATTTCCATCAACAAATAAAGTGTCTTTATTTTCACTAATTTCACTGTTTGATAGTGATTTTAGTGAATTTTTAACAAACTCATTTTGTTTAATATTAGTGTCATAATAAAGCACTTCTTTTTTACTAATATTATTGTCTTCACGATATTTTCTAAGTATATTTACAATAGAAATTATTTGTTCAATTTGTTCAAGATTTTCAACTTTTTGATTCTTTAAGTTTGGAAATTCTTGAGCTAATAATTCTTTGTTAAATAGTACTTTATATAAGTGATCAGTAATAAAAGGTAAAAATGGATGAGCTATTAAAAGTAATGATTTTAGGTTATCTAAAGCAGCTTTTTTATTTGGCATTATTTTTAACAATTCAACATATCATGAAGAAAAATCATTGTAAATAAATTTAGACAATTCTGTACCAACAATTGTGAATTCATAATTTTGCATCGCCTTATCAATTCTCTTTTTAAGAGCAACTAATTTATTATGAATTCAAATGTCAGCAGCTGTTTTTTTATTTTCTTTATCTTCAGGTAATAATTGAATATAACGAGCAATATTTCATAATTTATTACATAATCCTCAAGCTGAAGTTATTTTTTCTTTTGAGTAACGAATATCTAAACCAGGGCTTGTGTTAGTAATCATAAATCATCTTAAAGCATCAGAACCATTTTCATTTATAACTTGAATTGGATCAACACCATTGTTTGATGATTTAGACATTTTTTTACCAAATTGGTCTCTAACAAGGCCATGAATTAATACTCTATCAAAAGGTTTTTGTTTCATGAATTCTAATCCAAAAAAATACATTCTAGCAACTCAAAAAAAGATAATGTCATAACCAGTTACTAAAAGTGAGGTTGGATAATATCTTTTTAAAAGAGTTGTTTTCTTAGGTCAACCTAAAAATGAGAATGGAGCTATACCACTACTAAATCAAGTATCAAGAACATCTTCATCTTGAACTCAACCTTCGCCAGGTGATTCAATTTGTACCTTAACTTTGTCATCTTTATATCAAGCAGGAATTCTATGACCTCATCAAAGTTGTCTACTTATTGTTCAGTCATGAATGTTTTCCATTCATTTTTTGATAACATTAACAAATCTTTTTGGATAAAAGTTAACTGCATCTTTTGATTTTAAGTGGTCTAAAATATCTTGAGCTAATTTGTCCATTTTAACAAATCATTGTGGCATAACAAGAGTTTCAACTGGAGTATTTGAACGTTCTGAATAACCAACATTTGAAGTAATGGTTTCTACTTTAACTAATAAATTATTATCAGCTAAATATTGACCAATTTTTTCTCTTGCTTCTTGACGAGTTAATTTGTGGAATTGACTTTTTGGACAGTTAATTAAACCGTCTTTATCAATAGTTTCATTAATTTGTAATTTGTGTTTTTTGATAATGTCAATATCAACTTCAGCATGAGCTGATAATTTCATTAAGCCACTACCAAATTTCATGTCTGCATATTCATCAGGCACAAATGGAATTATTTGTTTAGTTAAAGGATGAACAATTTGAATGTCTTTAAAATCTTTATATCTTTTGTCTTTTGGATTGTAAATAACAGCAACGTCACTAAGCATAGTTTCAGTTCTAACTGTGGCAACTGTTAAATATTTATTAGAATTCAAGATTGGATACTTGATATAGTACATTTTTTGTTCAGTAGGTTTATTAATAACCTCAATGTTACTTAGAGCAGTTTTTAAAACTGGATCTCAGTTTATTGCTTTAGTGCCATGATAAATTAATCCCTTTTTGTATAAGGTAATAAAAGCTTTTAAAACTGCTTCATTACTTTTAACATCTAAAGTAAATCTTTCTCTTTCATAATCTAAAGCTAAACCTAACGATGCTCATTGTTTTCTAAACAATTCAGCATATTCTTCTTTTCACTCTCAAACACGTTTAAGAAATTTCTCTCTTCCTAAATCATGTCTGTTTAATCCTTCTTTTTGATAAATTGTATTTTCAACTTTGCTTTGAGTTGCGATACCAGCATGATCCATTCCGGCTACATAAAAAACATCATAACCGCTTAATTTTTTGTATCGAATTATTGTATCCTGAATATATGTATCTAAAGCATGTCCAATATGTAATTTACCAGTAACATTCGGCGGAGGTAAAAGAATTGTAAAAGGTTTTTTGCTTAAATCATGTTTACTAAATATCTTTTTTTCTTTTCATTTCTTTGCTATATCACTCTCTATAGAAGAGTGGTCATAAATTTTTTCAAGCTCCATACTGCTCCAATCTTTTTTAACTAAATTAATTAAAAATTAAACTTTAAAAACTTAATTTTTTGTGCATATAAATGCATAATTATATATATAAATTATAATACAAAACCTTAATTTTGGAGGGCTCTTTTTAAGCAAAATTTAAAACTAATTTTTAATAATTGAGTAAAGAATTACTACTAAAGTTAGATCTTTTTATCATCCATTTTTAATCCCTGTAATTTTTCAAAATCACTATAAAACAATTGTTTTAAAAAATAAACCAACTTTAAAAATGGTCTATTTCTTAAGTTCAATAAGCATATTAAGTTCCGTCTTAAATGCTTTTTATTTTTTGTATTTGTTTTCACAAACTCTTGTGATTCAACCAATGAATCAGAAAATTCCATGAATTGCATAACCTAAAACAAAATTAAATATTAAATATAACAATGTAGCTACATAATAATTTTTGCTTCCGTTAATATGTAAAATATTTTCAAACATTTTAACAAATGAAATCTCTTGACGATGACTCATTAAAAAGAAGTCAGGTAAATAATTAGGGAATCATTCTAAGTTATTTTTAGCTGTTTGATAAACAATTTCATTCCCTATAACTGCAAGTGACCATAAAATTGTAAAGATAACAAAACTATTAACAATAAACATTCCCTTTTTGTATTTTGCTCAATTATTAACAACTAAAATCATACCGATAGCTAACATGCAGCCATGATACATCATTGTATGGATTGAAATTAGTATAACTTCATGAAATACATCGCCTGGTCAAAAAATCATAATGAATGCTCCACCATAAATTGTTATGTAAGCTAAAAAGTCAGTTATGATTTCTTTAAATTTAGTTTTCTTTCTTAAAAACAAATAAAGAGGTACAAAATATAAAGGAAGTGAACATATAACAACTGGATAAAGGTTTTGATGATAATCTCATTTCCATTTATATTCTCCTGCTGTATTTTTGACAGCTTTTCCTGCTCATAACATTTGTTTCAATAATTCCATAAAGACTATAATAAAGAATAAAAGTCCAATAGTTAATTTAACTGTTCATTCTTTTCTATTTCTTAAAGCAACACATAAAATGATGCCTAAAATAAGAAATGTTGCTAAAAATGTCAAGTGGAATGCGCTAAAAGCTTCTGGTCTTCCATTTGCAAATTCAGTATTAAATTTTAAATAAAATTTTGACATTGTATCCATGCTTAAAATTATACAAAAAATATTGAATTTGTGTCCCACAAACCTTCTAGGTTTTAAAACATTTACTTTTATGTAATATAATTAGTAATATATAAAAATAAATATAGGAGTTAAAAATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGCTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATATTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCAAGAAAATATGATGAATTTATCGATGCTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGATACAAGAGTTTGATTCCATAGAAATGGAGCTTACCGTGGATCAGTAGATTTAGCAAAAGCAAAAATAATCTTAGATCGCCTTACTCAACAAGCAGTTGAAAAACAAAGAATTATTGAATATCTTGAACAAGAAAATCTTGTTGATAAACCAGAACCTAAAAAAGTAACTAAAAAAGTTATTATTGTTGAAGAACCTAAACCAATTGATCTTAATCAAGAAGCTAAAGAAGCAACATTCTTTGTTCCTAATTCAGATTTAATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTACTGAAGAAATAATGACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGAACAATAATCACAATGTCAGTATTGCTTATTGCCGCTCTTACTATCTTAGTTTTAGTTGCAACCCGAGTAATTTAATTAATTTTTTGAAAATAAAAATGCTCTTATTGAAAGGACATTTTGTTTTTGTTTTCTGGTATCTTATTTGATACTTTTATAATTATAATTAAGCTATATACAGCTAAAGCTAGAACAAAATTTATTACTGGATAAAGCAACGTAATTACTCAGTAGTTGCCATTTAATTTAACACTAAAAATTTTCTCAAATAATAGTGTCAAATGATTATTAAGATGATGACTAATTGCTAATAAATTTGGATAATTTTCTTGTAATTTATTTAAGTCTTTGTGTGCTGTTTGATAAAAAATTTCATTCAATATTACTACTACACTAAACATTATTAGGAACATAAAAAGATTATGAATAATATAATTTCCTTTGTCGAATTTTGAATAATTATTAAAAATTAAAACAATACCAATTAACATTAAAATTCCATGATGAATCATTGAGTGACAATCAAAAAATATCGATTTATTTAAGACTTCATTTGGAATAAATATCATTATTGAAATTCCGCCATAAAGGCTAGTAAATGAAACAAAATCGATCAAAATTTTATTTAGATTTTTGTTTTGATTAATCAAATAAATAGGAAATAAAAAGTAAGGAATTGAACAAAAAAATAAAGGAAAATAAGATCATTTATATTTTGAATATCATACATTATCTTTATCTATTTAACCTAAAGCACTTAGTTGTTTTATTATTTCAAAAGTTACTAAAATACCTAAACAAATCAAAATAAATAGCTTAACTTGTTTATCATTTTTGTTTTTAAATTTGTAACAAATGACACCAGTTAAAATGACAAAAATACCAATCACGATAAGTAAAATCATCCATAAGCTTGTGGAGTATTTGAGAAGGATCACGATATTACTTTATAAAAATTTGCCATGATTTAACAACCTTTGTTCTAATAATATTTTACTAAAAAACCTATAAAACAGTTTTATAGGATTATATTAATATATGTCATCTGATAATACTTGAGTCATTGTCAAGTCAGTTTCAACTTTTTGATTACTATTATTTACTTTTTGAATAGCTTCTAATTCTTGAGTAAATGTTTTATCCAAATTTTTGTTATTTTTAATTTTATTAGGCTGGAAAGCAGCTATCATAATTGTCTTTTCTAATAAAGGATTCTCTAAATCATTTATTTGTGTTTCAATAGTTTTTTCTTGACTCAATTCATCAGTTACTAATAAAGAATTATTTTCATTTTTATGGCCTTGCATTCATAATTTGAAATATTTTGAAGGTGTAATAATGTAGTGCTCTTCATCTACATCATAAATATCACTAAAAACAAATTCTTTTTGACTTCTAATAATTTCTTGTGAAATGGCTTGGTCATTAATTTTACTTTTAATTTCAGGGTTAATTGTGTTATTTAAAACTTCTTTTAAATCTCCAAATTCAACTTCTTTATTATTAGCAATCAAATAGATATAGTCAAACTCATTGTCTTCAACTAATTCTTTATCTTGAGTAACAAAAATAATATTTATTTTACTTTGTTCACATAATTCAAAAGCAGCTCTTAAAAATTCAAATTTAAGTTTTGTATCTTTAATATCAGGATCATCAGCAATAATAATTAGTTTTGGTTTCTTAATAATAGAAGAAATAAAGTTCATTTTTAGTTTGTTAATTTTTGAAATATTTTCATAAGGTCTAATTAAGCTCTTTGTATTGATTGATGATTTTTCAAAAATTTCAATTAATCTGTCAAATTGGTCTAATTTGTGAAGAATTTTATTAGAAACTTTTTTAGCTAAAACTAAACTATTTTTAAGAACAATAACATTATTAAATACAAAGTCAAACATTTCAAATTTTTCAATTATTTTCTTTAAATTAGCACTAAAATTTTTAATGTTTAATTTGTCTTTTTCATTAATTGAACGATTAAAAATGCGTTTAAATCACAATGAAGTTTTTAACTTCATGATTTTTCTTTTTATTTTTGAATTAATCAAAGTCATAGTGTCATAACTGTTTAATAATTTTTGATTTGATTTTAGTGTGTTATAGAATTCTGATTGAATTTTGTTGAATTTTCAATTATGTTCACTTTGAGCATTAGTTAAAATTTCTTTTGCAACTTTGATAGATCTTTCAGGGACAGTATTGAAGTATGTTCCATATGATCTATTTTTAATAGACATTATTTGACTTTGAATTTGATCTTTTGAATCTTTAATTAAGTCAAAATTGCTTTTTGATTTGATTTTATAACTTTCGACATTAAAGTAGAATTCTTCTTTAAGAATCTTTTTAATTTTTTTAATTGAAAAGTTTTTATTGCTGTAAGTTAAATTTCCTAAACTATTGCTAATAAATAATTTGATGGTGGTATCTAGTGTTCTTCTCATTTCATCAAGTTCATCAATTTTTAAATATTTAAAATATTTTTTATATTTTTTAAGTAACTTGTGAGCAAACATTTTAACAAAATATCTTTTGTTTAAAATTTGTCTTTGTGAGCTTTCTTTAGCAAAAAAAGTTGATTTATACAAATCAATTTCGGCACTTCTTTTATATCTAAAAGCAAAGTATTTAATTTTTTGATCTGAGTTGTGACAAATTTCTTTTGCTATTTCATCTAAAGCACAAATATCATGTTTTAAGTCTCTAATTTTTAAGTTGTCTTCCACTCTTTTTTTAGAAGTTTTTCTCATTTTGGTTAAGTAGTAAAGTCTCTTTTTTAAGTGATTTATTTGTTCATCACCCAAGTAGTCAATATTTGATTTAGATGCATTATAAAAGAATTCAAATTTATTAAATACTTGAGAAAACAAGTCTAATATTAAAGTAATTTGTTCACCAAAAAATAAGTCTTGGTAGCTTTCAATTTCTTTAAATCTTGTTTGTAATTCATCAAAGTTATATTCAGCAATATTTTTTGTTGTTTTATTTAAAAAACTATTGAAGTTTCTTTCAAAATCACGAAAAATCGCCATTAATGAGTTAGCTTGTTCTACTAAAATATTCAAAATAACATGACGAACAGGATAATAGTATTTATTAACTGTCTTATTTAATTGGTTAATACGTTCCTTGTTTTCACCGTAATTTTTTTTAAGTTCTTCATATTTGTAAACTAAAGGATATTGTAAATTTTGTTCTTCTTTTTCAGCCATATTGAAAAAGTTAATTTCACTAAAAGCATTATTATTTTTATTGAATTCAATGTTTCAATAATTTGTTCCTGAACCTTTTTTATTATTCTTAGAAAAGAAAGATAAAATGTTCAAATTGTTATTGAAAATTGTATTGTAAAAATTGGAACCAATTAGGTTTCTGTTTTTATCAGTAACTAAAATTGCAGTTTTAAGTTGATCGTAAATCTCAATTTTTTGTAAATCTACATAATCATCTTCAAAATTTAGATTCTTAAAACAATTTTCAATTCCAAATATTTTATTTTTCTTCATATTAATAAAAAGTATATTAAAAAAATGACATTTTTATAGCAAAATATTATATAATATTTTTTGCATGGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGACATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCATATGCTGGCTGAGCCAGCTTTTATTTTTTTAAATTAAGCTGTTTTATAGGCTTATTAGTCAAAATAATAAATAAAAAATATGTTAAAAATTTTTAATAAAAAATTTATAATTTATTTAAAGGAGGCTTATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGACAAACTTATTGAATAAAAGCTTCTATTTCTAAATTAAGTATTAAAAATCATGCTTACTTAAATATTGTTGATGGCGAATATTCATCTAATGCTGCAATATGAGCAAGCAAGATTCCGAATATTGGAATTGATATAACAAGTAACAATAAAGATCTTTATAGTCATGCTTATTTTATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGTTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAAGAATTAGTTAAAAGCATCAAAGAATTATTCTTACAAAAAATACAAGCAAAATTTGAAATGGCTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAAGGAGAAATTATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAATATTAGTCAAATTGAGTTCTTTTTTAGGACTTATTTTTTTAACTTTTTAGAGCTAATTTTTTAACCATTTTTTATTTAAAAAATAGTTATTTTTTAATATTAAAAATTATTTTAATTATTTACTTATTTATAACTATTCGTTTATATATAATAAATAAATATCAGGAGGGAAAAATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGCGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGAACTAAAGAAAATGATGTTCAAATTACACCAAAAATGGCAGAAAACTTGGCAAAAGATTCTATTAAATGACTTAATGTTTATAAACATAGAATATTAGATGCTAATGGCAACTTTATTAAAACTTGAAAAAGCAATGCCAAAAGAGTTGAAGAAATTTATTCAGAAACAAATATTTGACCTTTATTTATTGGGACTTATTTTGTTAAATATAGAAAAGATGAGGTTTGTTTATATGCACCTTATATTTTAAAGGCTGTAAAAATTACAATTAGCAATCAATCAGTTTTCTTAGAAAATGCTGATCAAAACATCATTATTAATGAAAAAGTTAAATATTTACTTGAACAACACAGTCAATTTAATTTACCTATTTTAGTTGACGGCGAAGAAGTTACTTTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTGCTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTATTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGAAATGTATCGAAAAAAATCATTAAATTCTACAATAAAAATTTAAGTAGATATGACATCAATGATCTTGTACAAATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCGGCCAAGTAACTGATTTAATACTCGTGATGAAAATGAAGATGAATATAGTGAAGAAGAAATTACCTCATTATTAGATTGAGCAATTCATAATCACTTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGAGGGTCAATGAGTTGACAATAAAAATGAAGTTGAATGTGAAAAAATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGATTAAACTACATTCAATTAAATGAAAACAGACAAAAAGAATACGCAAATGTAGTAGCAGGCTTAATTTTTAATGATAAAAGCCAATTTATGTATGAGTTAGAAAAATGAATTAAAGAACAAAACTTTTCAAATGAAATCAAAGTTGTCCAAGATTACTGTGTAGGTTCTTATAACATTGATTTAGCAATTTTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAATTATTAAATTACTAGAGTTTTATAGTGCTCTAGTTTTTTTAATTTGCAATTTTAAAAAGTGAAATTTAGGCCTTAAAAAATATCTTTATGTATATTTTTTGCTTGTCTTATTACCTTTTTAATATATACTTTTATTATTGTTATAATAATAAGGGACAGAAAATGAAAAAATTTGTTGCTAATTTTGTATTAAACAATTGAGGAGGATTATCATCAAATCCTGCTTTTAGTAACAAATATGTCGAGTTCATTTATCAAAAGTAATTAATGCTTAAATGTGCTCAAAAAAGGCACATTTTTGTTTGCTTTCTAAATAACAATGCAATATCAACGAAAGGAAAATATGAAAAAACCAAAATTCAATTTTCGTACTCATGTTTGACCTAAATATGTAATTTCTGCAGGTGTGCTTGCTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGATTTATGACCAATGTTTCTTGAGGCCCAGAAATTATTACTCTTAAATCATTCTCAATTGTTAAGGGTGTTGAAATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGAGTTATTGAAGGTAATTGAAAAGAACAATTAAAACAATACACCTTAGATAATATTGAATTAAGCAGATTAATGTGAGCTAATCCATATTTAACTTTAATTCAAGGAAAAGATGAAGAAACTGCTAGAAAGAATTTACTAATTGCTTTAGAAAAATACAAAAATACAGATGAATTTAACATTATTAAAAACATTGCTCAAAATGTTAAATTAGTTCAAAAAACTATTACTAATATTCATAAAGGTTCCAACCCTATTGCTAAAAATGCAAATATTATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGAGTTAAAAATGATAAAGTTGTAAATATTGCTCGTGGTTACAATGAATCACTTGAAAGAATTAACTTATTAAAAGAAGAATTTATAAACAATATTAGAAAAATTGCAAACAAAAATGTTAGCGAAGAAGAAATTAATAATTATGTTAAAGCGGTTATTGCAATTCAACCAACTATTCAAGAAATTACTAAATTAAATGAAACTAAAGCTCAATTAGATGAAGAATTTCAAGAACAAGAAAACTTTAAAGCTCAAGTTGCAAAATTTGAAGAAGAAATTACTGATAAACAAAATAAAGTAGTTCAAAAAGAAACTAAGCTTAGTGAAGTTAAACAAAAAATAAATCAAAAGAATAATGAAATTAAAGATTTAGAAGAACAACTAAAAAGTGATCCTTCAAATCAAGACTTAAAAGATAAACTAGCAAAAACTAAAGAACAACAAGAAAAACTTGTTAAAGAAAGAAATGAATTATTTAAAGAAATTAATAACTTTAAAAACAATATTAATGCAACAAAAAAACAATTACAAAATGTAAAAAACAAGCTTTGAAAATTTATAACTCAAAAAGCTAAAGAAGACAAACTTAAAGAAACAAAAGAAACTATTGATGCTAAGGTTAAATCAACTACTGAAGAAATAGAAAAAGATTCAAAAATTGAAAAGAATCAAACACCTGAAGCCTTAAACGCTTTAAGAAGTTTAGCACAATTAATTCATGATTTTTATGAAGAAAAAGATGTTAAATTACATAAACAATTAGATGAAAGCACAGAAGATTTAGTTGCTCCAGCAGGCAAAAATATTTATACATACAAAAAAGAATTAGCAAATAAATTTGACCTAATTCATTTTGAATATAATGAAAAAATCAAAAAAATTACAACTCCTTATTTTGATGGAAAAGCCTATGAAACAGTAAAATTTGTTCCTTACTCATATTACACAACTGATATTGCATACTTACCAAACCAACTAATAGCTATTGACTCATTAGTTGATATTTCACGTCTTCAACAAATTAATTGAAGAAACTTTTATAACTTAGATGGTTACATTCGCAGCAAAAATATTGATGAAATTAGTAACAATGATGGCTTTTATGTTTATACAAAAAATGTTTCATCACTTGAAGAAGATTTCAACAAAAATAACCCAGATTTAAAATACAAAATAGAATATTCAAAAACTATTAAAAATAGAAAAGAATTACAAAACAAACAAAAACAATTAATGGCTTGAAATACTGAATTTACTGAATTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAATAATGAGAAGAAACTTACAATAAAGTAGCAAACAAATCTTGATATATAAGTACAAATCAACTTATTTTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGTTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGAGAAGATCTAAAAGCAGAATACACACCTTTAATTAAGCCTTACAATGATGCTTTTGAAGTTGATATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGAATTAATGAAATTAATAGTAAACCAATTTGAAATATTGATAAATGAGTTAAAACACTTATCAATGGTCAATTGAGAAGAGCTATTAGAGAACGTACTGATTGATTTAAAAAGGAAATAGAACAAAAATTAAATATAGCAAAAAAAGCTGAAAAATTAGATGAACTCAAAGGAAAAATTAAAAAGGCAGAAAATGAATTAGCGCCTATTAAAGAACGTTTTGATACTAAAAAAGCAGTTTTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTTTAGCTAAAAATTCAGAAATTGGTAATATGATTCTCGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGAATAATAAAAAATGTAAAAGGAATTCATTACCAATTAGAATTAGATAAAAAGGCAAAATTAGAAGAAATTGCAAATCAACTTAAGAAAATTAAAACCTTAACAATTGATTTAATTAAAAAAACAGAAGATATTCTTGGCCACAAAGATGTTGTAAATTGCATTGATAAAATCAATTCATTATTCAATCAATTTGTTATTAAATCATCAGGTTTAATTTCACCTAAATATATTAATGATCCAGTGATTAAAGCTCATTTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGAGCTAAATTTTGCTACTTCACAGCAGTAAAAGTTACTAAAGATCATTTAGATAATCAAGATGCAAATCAAATTAATAATTTAATTTCACAAAATAATGCTGAAATTAATTCTATTAACAAAAGAGAAATAGAATTAGATAAAATTATTAAAGAATATGATCAAAAAATTAAAGCAACAACAGATTTAAATGAGAAAAAACGTCTTAATAAAGAAAGAGGACCTTTTGCTCTTGAAAGAGATAGAATCAGAAAAAAAAGAAATGAAATATCAGCTCACAATACGTATTTAAACAATAGACTTAAAGCTAATTTATTTGATGCCTTTAATAAAGCTAAAACAACTTTTGGACCTTCTGAACTTTTTGTTGGAGCAGACTTACATTTTGATATCACTAAAGCATTAAACAAAGTTCAAAGAATGAGATGAGAATTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGACTTGAAGGTGAACATTTAGTAAGCAAATTAAATGAAAAAGGTGAAATTGAATATACAGTTGAAAATGGTAGATATTTAGGTTTCACAAAAGATAGTCGTGTTAGTTTATGAGCTATTTTAAAAATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGAGGTACTAAATTAAATAAAGACGATATTATTGCAACACTTAAAAACAATAAACGTCGTTTCTTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGACGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGAGATAAAACTGAACAAAGATATGTCTTTAAACCAGGAAGTTTAGAAATACTTAAAGGTATTTTAAAAGATGGCAAAGGCAATATTGTTGAGTTTGAAGAAGTTAATGGTGAATTATTACCTAAAGTTGTTGAAGGCGTTAGAGATGCTAAAGGTAATTATGTAATAATAAACAAAGTTTTAATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGAGTTGAATTAGTCAATAATTTAACTTATAAAGCTTTTGTTAATAATAGAAATAACAACACAGGTGAATTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGATATTCTAAATTTACACCTGAGTTTATTGGAAATAATAAAAACAAACATTTATGATTTGCAAAAGACAAAAATGGTGAAACATTAAAAAAATTACCAGCTTTAGGTGAAATTCATGAAGTTTCAAAAATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGAAATATTGATTATGTAAAAGGCAAAGTTGACTTCAGTAAATTTATTGCTGGTTTAAGGCTTGCGATAGATGAAGATCAAACCCTTGAAGCAAAAGAAAAAGAGGCCTATACAAAACTTATTCATAATAATAATGAGCAAGAAATTGCAAATGAAATTATGTTTAGATTCTCGCATTCTAATTTAGGTATGTTAATGTCAAATATAACTATTCAAGATCTAAAAGATAATAATGATTATGGTTGAATATTTGATCAAAACAACGGTTATGGAGATTTAAGTGTTTCAACACTCAACACAATTAACCCTGACATTAATAAATTTGAAATATCACTTGAACAATACATTAAAACATATGAAGCAAAAGCTCAAGAATGAAGTGCTCAATTAAATCAATTTAACTTATTTGATGCCTTAATTTTTGATGGCAAAACATCATGTTATACTTCTCAAACAGTTGGAAATATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGATTAGACTTATTAATTAATTTTAGAGGTGACAAAAATGGTTCTGGTGTTAGAGATACAATTTTAAACTTTGAAAAAATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGATTCAAAGACCGTTGATATCGTGATTTCTTAGACTTTAAACTTTATGATGATCAAGGTAAAGACATCAAAGATGATACTATCAGAATTAAAGATTTAACTGGAACAGTAGTTAAAACAAGAGCCAGAGCTTACTGACAATATTACATTCAATCTCAAGGTGTTGGAAGAAGAAATATCACCAGCATTTGAAGAGATTCAGATAAAGATGCAGTTGCAATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGAGTTTCAGATTATGCAATTATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGAGATTCAATCTCAGAAAATGGCAAAACTTTCTCACAAGCACCTATATCAATTTATAGAGATGCTGATGGTAACTTTAGACTTAGAGTGCAAGATCAATTTAATGGGGTTATTTAACCAAAATAACTATAAAACAGGAGGTTTAGAAAATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGCGAAAGTAGATGAAGTAATAATGATATAGTAACTGCTGATGATTACATTGATGCAATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGAATTTATGATGTTTTAAATCCTAATTACAAACCATGAGCTTGTCAATTAGAAAATGAGGCTGATAAAGCTGAAGTTGAAAAAATTAAAGAAGAAGCTCTAAAATTAGGCCTTTATTCGGGACGTATGTATTGAAATTATGATAATCAAACAATCTTAGCAGCCATTCCTTATTCACCAGACTTTAACGAAAATGATGAAATTACAACGGTGATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTAGTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGAACCAACAAAAGTTATCGTCCATTTATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGAAACTCATCATTCCCAGTTATTACTTGAACTGCTTTTGGTCAAGGATCATCAAGCTTTGGTGATGCTGTTGAAATTGGTTTTGATCATGACTTTATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGAACTTACCAAAACTACTTTGAAAGCTTAGGTTATGTTTGAGATGATAAAACTCAAAAATTAATTTCAAATAATGCAGCTTTAGTTGATGAAACAATTAAAAGACTAAATATGGAAACAAAACAATGAAACAAAATTCTTGATTTAGCATTCAGAAAAACTTATGTTGATCAAAAAGATGCTAAACATGAAACAATTACTAAATTTACTGAAAGATATTTAAGATTCTTTTCAAACCAATTCAATGAACAAGAAAAAGCAGAAGGTTGAACTGAACAAAGTGCTTTTGGTATTATTACTGCTTTAGAAAAAATTATTCGTGATGCAGCTCCGGTTGTACCATTAATGGAAGTTGATACTTACTGAGAAATTTCAAGAGTTAATGGAACAGAAAGCTTATTCACCTACTCATTACAATTTGCTTATGACGTGGCAAAACCTCCAAGAGCAACCTTGCCTACAGTCATAAAATCTTAGAAAGGAGACAAAATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGATGATCAAGAATTTTTAACATTCAATCTCCTGCTCTAAAAGTGTTATTCAAAATTTCATGAATGATTTTAGAGTTCTTACTAATTGCTTGAGTAGTTATAACAATTACATTCTTCTTAATTAACTCTGTGCCTGGTTCAACTACTATTTCAGCTGGACTAGATGATGCAGCTCGGCAAGCAGCTGAAGCTCAATATGGACTTAACTTGCCGCTTTATCAAAGATATTGAATTTATTTAGGAAACTTACTTCATGGAGACTTTGGAGTTTCACTCTCAGTTTTCCCTGGTGAAAAAATTAATTCATTTGTCTGAGTTAGATTCCTTAAAAGTTTCATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCGAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGAACTCATGCTCTTAAACCTTCATTATTCCCTATTGCTACATTCTTCCCTGTTGTCATCTTTGGTAGCTTTATTGGATCAATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAGAAAGGAAAAACTATGAATTGATTTAAAACTTGAGTTGAAAAGAAAAAACGTCGACAAGAAAGTCCAAACAATATTCATGGTAAGGACTTAGCTCCAAATCCTTTCTTACAACCATTAAACTATAAAAAATGAGAAATTATCGGTAACCTACTTGAATTCCATGAAACCTCTTCAATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGAGCAAGACTGTGATGAGGTTTAAGATATTCATTAGCTTTAGCTTTTGTAGCAACATTTATCCAAGTGTTTGTCGGCTTAGCTATTGGTATAATGATGGGACATTTTAGAGTCTTTGATAAAATAATGACTTTCATTATTAAAATTATTTCAAATGTGCCTTCTATTATTATTCTTATTGTTATTACTATTATTCTTAAACCTACATTTTGAGTTATTGTCTTTGCTTTAACATTTACTTCCTGAACTGGAATTGCTAACCAAATGCGTTCACAAGTTATGCGTGCTAAAAACTTTGAATGAGTTAGTGCTTCAAGAATTCTTGGAACTCCAACCTACAAAATTTTACTTAACTACTTACCAGTAGTTGTACCACTTCTTGTTACTGAAATAGTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAATATGGAAACAAAAGAAAAGAACAGAGGGTATTCAAAAATATTTAGAAAGCCTATAGTTTATCAAAACAAACCAGCGCCGCTTGACTTGTTTGAAGTAAAAGGCAATCCTAAAAACAAATTTTGAAGAAGTTTTAAAGATGCTTGAATTAACTTCGGAAGAAATATAGCTAAAGGTACAAGAAAAATTGGCGAATGATTACATGTTGTTAAAAAGCAAGAAAAAAATAAATTAGATTTTAATCAATACATTGAGAACGCACAAGTTGAAGATGTATTCGGAACACCTACAAAAGTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAACCTAAAATTTTAGTCATGGATGAGCCCACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCAATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGAGGCTTAATTGTTTCAATGCCTGACTTTAATAAAGGTACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCAGTTAGAAATGACTATGCTTTAGGAATAGATTTCGAAGAAGAATCACCTTTCTATCAAATCTCTCCTACACACTTTGTTAAAAGTAATTTGCTTAATGGACAAGCCCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGAAATAAATACAATTCAAAATTAGAATCTATTTATGGTACTGACAAATACGTTGATCAAAAAATATATGATAATTTTGTCCAAAAAGCCTATGAAAATAATGAATTGGTAAAAATTTTAAATACTAAAAGAGATGCTGAATTAGCTTCGAGAGAAGAAAATAACCAAACAAAATCAGGAGCAAATAATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGTACAGATGAAATGCTTAATGCATATCCAAATAAAAAAGCTTTAGTAGAAATAAGAAATTTAGATATTGATTATGGTTTTGGAGCTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGCGAAGTTTTAGGACTTGTTGGTGAAAGTGGCAGTGGTAAAAGTACCACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGAGGTAAAAAATATAAAGAAATAATTAACTTTATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAATTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACACGCTTTGATCAAATAGTTTTAAAAGAAGTAAATAGATTGATAAATAAAATTGATTCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATAACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAGATAATTTAAGTGAAAAAGAATGTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTGTACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATCGCAGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAAGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCAGATAGAATAGCTGTTATTAATAAAGGTGTCTTACTTGAAGTAGGTCCAACTGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCCGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGACAAGATTTAGGAAATAATCACTTTGTGTTAGCTACCGATGAAGAATTAGCTAAATACAAAAGAGATAGAACTAGACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTGAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGAAGATGATGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAAACATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAAATAGTAGAAAAACTTGCTAAAGAAAATGAATAAAAAATCGCAAGCAACTGCGATTTTTTAAACCTAAATTTAAACCATATTTTTAGTTTTTAAAACATAAGTTTTGAAAAGTGTTTTGTCTTCTGAAACATATTTAAATTTAAGTTCTAAATGATTTTTGCTTACAAATTGATAATCAAAAGAAATTTTACTTTGATAATCTGCGTTTAGTTTTTGAAAAACATCTTTCACTATTAAAGGCACAACATTTTCATCAATATATTCTTCGCCTTTTTCACCTATTCTAATGTACTTGTAATAGTTGTTATATTCTAATTTAGGAAATTCTTCAAAAGAATTTATTGTTTCTTGTTTTTCAATTTCCTTATGTTTACTTTTAGCTGCAGATACAACAACAATTGGAGTAACAACAGCTAAAACACCAACAGTACTTAAGAGTGGAATTAAAATTTTCTTTTTCTTAGCCTTATCCATTTTTTACCTTATATCTGTCTAAAGAATAAATTGAAGAAAAGTCTTTTAATTTTAAATATTCTTCAATATCTTCTCCTTTAAATTCATATGTTGAATTTGAATCAAAAATATCATTATTTTCTTCGTATTTTAATTGAATTGTTCCTTCAACATGATCTAATATTTTTCTCTTAAATAATTGGTGATTGTTGATATTTAAAATAATAGGTGAAGAAGTATTTGAATATGCTTTATCATTGAAATAAAGAACATAATTTGTTTTTAAGTTACCTTTGTAATTATATGGAATAAATAGACCTGCTTTTTGACTTTTTGTTGAATTTACTAAATAACCATTGTCATAATCATAATCTATGAAATTTTCGTATGTGCCTGTTTTATAGTCTTTTCACTTAGTTGAATCGTCACTACTTGTTGTGGTTTTAGTGTAGATTTCATTCTTAACTTTTGTAAATAATTCATTTGCAATATTCTTGAAGCCATAATTAGTTAAATTTTGAGAAGCATAAAAACTTTTGCTAATCTTTAAAAGATTTAATAACCTTGGGAATTGTTCATAATATTCTTCTCATGTAATTCCATGTCAAGGTGCAGTATAACCATAAGGTCAGTGATCTGATAAACTACTTTCTTTTGCTTCTTCAGTCTCAATAGTTGCTAATTCATTTTTTCTTTCATATCTATCAAATTCATTAAGTTTAACTATTGAGTTTAAATAATGAATTTTAAGTTCATCTTTTTCTATGTGACTATTGTAACCATTGAAATTAAATTCATATAGTTGAGGTATTTTGAAATATACCTTATTGTCTTTAGCATTGAAAGTATATTCTCTTCTTTCATCATTGATTTTCTTGATTTCTCAATATTGTTTTTCTGTTTTTTCTTTACTTCTAAAGTAAACTCTTAAAATACGGAATTTTTCGTAAGGTAAATTTATAATTTTAGGTGAAAGGATTGTTAATCCATATGCACTTGTTTCTTCAGTAAATTTCGAATACCATTTTTTAACACTAAGTCTTTCATTTAGTAAACTGCTATCCATTGTTTCCAATTTTGTGCAGCTTCTAATTATGTTTCCATCAATTCTTTTTCCATTTATTTCAATATCAAAACCTTCAAAGAATTTTTTATGTAAATTTTTGGGTTGAATAAAAAATATTTTAATTGCAAATCTATCTTCTGTAAAATATCTTTTTATTTCAATATTACCATTTACAATATTGCATGCATCTTTAAAATTTACATCATAGTGATCTTTAGTTAAATAAGATGGAGCATCTAAATATTCTGATTTGTATACATAACGTTCTTGCGAAATATCATGCACCAATTGTTGTGGTGGTTGAGGTGGATTATTCGTTAAAATAATGGTACAAAAACTCCTCTACTGCTTGTTTTTTATACTTATAATAAGTATTTTTGGAATAATAATCTTTGAATCAATTTTTAGATTGACTTTTTAAGAAATCCTCTTTGATAATTTGCGCAAGTTCAGGTTTCATAATAGATAAAATATGCTGACTTTTATTTTTTGGTTTAAAGATGTTTTTAAGTATGTTTGATTTGTTGCCTCCTCCAATTGTCTTTTTGTAAGCTTGCTCATTGACTTTTTCCATTTTAAAGATATTGTGTAAAACACTATTTTGATCTTTATGTGAAATTAAGCCCATAAAATAATCATTAAAAGTTAGATTCATTTTCCTCCTTTCAATTTATATCTTTTCAATATAAATAAAGAAGAGTCAAAAGCGGAAAAAAATAGGAAAAAAGCATGATTTTTCATAAATTTTTCCTATTTTTTAATTTATTTAATAATTTTGTACACAATTTAAAGAAGTTGTACGCCTTCTTTAGCTAAATCATCAATTAATTTTTTAGGTCAAATACCTACTTGAACTTCACCAATGTGTTTCTTCTCTAACAAGAACATTGAAAGTCTACTTTGCCCTATACCACCGCCAATAGTTAAAGGGAAAGTATTATTAATAATATTTTTATGATAGTCTTTAAATTCATCTTGAGAAACATGACTAAATTCAGCTTGTTTTAATAAGGAATCTTTATCAACTCTAATTCCCATTGATGAGATTTCTAAAGCTCTTTTATTAACTGCATCATAAACAATTAAGTCACCATTTAGTTTTCAGTCATCGTAGTCAAAAGCACGTTTTGATTGAGGCTTATTATCTGCTAAAGGATAACCAATTTCATAAATAAATATAGCCTTGTGTTTTTTAGCAAATTTTGTTTCTCTTTCTTCAGGGCTTAATTTTGGATATAAGTTGTAAAGCTCACTACTACTAATAAAAGTTACATTTTCAGGTAATTTTTGACTTAGTTGTGGAAATTCAACTAAAAGTTTGTATTCAACATGACGAATACATTTAAAAATTGTGTTGACTATTTTTTTCAAAAATTCAAGATTACAGTCTTCTTTTTTAATAATTAATTCTCAGTCTCATTGATCAACATAAAAAGAGTGTAGGTAATCTAAGTCTTCTTCTTTTCTAATTGCATTCATATCAGTTCAAATGCCTTCATAAGTACTGAAGTTGTATTTCTTTAATGCATATCTTTTTCACTTAGCAAGAGAGTGAACTATTTCAACAGTTTCATCAAAATTTTTAGGAACAAAAGATACAGGTTCTTCCCCATTTAAGCCATCATTTATTTTGGTATTTCTAGCAACAAATAAAGGGGCTGAAACTCTAGTTAAATGTAATTCCTTGTTTAATTTTTTAGTAAATGCAAACTTCAAATCTTGAATTGCTAATTGAGTTTCTTTAATGTTTAATTTACTCTTGTACATTTTCGTTAGCCTCTGTTTTATAGTTGTTTTGGTCATTTTGTGGAATTGAATTTTCTTGATTATTTTTAGCTTCTTGCATAGCTTTTTTTGTTGTTTTCAAGATGTGTAACATCATTGATAATTGTGATATGTAAATTAACAATGACAATGTTTGTCATAATAGTGTTGAAGTTAAGGCTGGAGTTAATTTTCCTTCTGCAATAATGAGTGATACTCAGTATGCAATTCAAAAAATATTTGTTAATACAAACATTAAAATTAAACCAACTGATAAGCCTTCAATATCTCTCTTTTCAAATGTTTTTAATACTTGTGGTAAGAATGCAAAAGTTGATATAACTGGAATAATTTGAGACAAAATAGCAGTTGTATTAGGATCAAAATTAAGACCATTGCCTTCACTGTTTTTGTGATATAAACCGACAAAACCAACAATTGCACAAATTGTAGTTAAAGTTAATGTTATTCCTAAAACTACAAATTTTCTTTTGTTTCTTTTTACTTTTTCATCATATTTGTACATAAAGAATAAAAGTACACAATAGACATAAGCACACAAAATATTGGCATAAACTACAGCCGCCATTTTAGCTTCTTCCCCTGTGAATGATCCAAATATTATTCAGCTACATAATGCAGCAAAAAATATTCAGAATGAATAGAATTTAACCTCCCCAGTTTTCTTTCTTTTTAATAAATAAACTAATTGAGGAATTGATACACTTATTGTTGCAATACATGCTATCATTCCTACTATTGCTACAAACGCTGCTATGCCATCATTAACATCTTTATTTATAGCAAAAGAACTACGTATAAAATCCATGTTTTAATTATATATTAATAATGTTTATAAAATAATAATTGAAATATTAAAAATGTTAATTCCAAAAGTGAGATCTCTAAAACCAAGGGTCTCTATTTTGGAATTAACACTATTTTTTACAATAATACTTTATTTTATTTTTTAAGTTTTTATTATTTTTCTAAAAAATAATCATAAATTTATTTGGTAATTAAATCGCATAATTTTCAGTTTTATCTACTTTTATTTTAAAATTAAACTATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTTTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAAATTAAAGCTTTTTATTATTATATGGATTGCGGATATAGTCCTTGCAAACCAAATAAAGAATATGTTGAAATGTGCTTAAAAGGATACCAAGACTGCAGTATTAAAAAAGAACAACTTAGAGAGATTTTATACAAGTAATATACAAGTAATATACAAGTTGTGTTATAATCTTTTTATGATTAACAAGGAATATAAGCCTATATTCAAGGTTACAAACAAAATAATTGAATTAATTGGCAAGATTAAAGAGAAATTGTCAAGCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAAGGTGCTGTAAACTAATTGTGGGAATGAATTTAAATGAGAGAACAAGCATAAAGCTTGTTTTTTTCTTCTTTCTATCAAACTAAATATAGAAAGGAGGTGAAATTATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGAAAGAGTAAAAAGTATTTAAATTTTGCTGATAAGGCATTGATTTTTTTATCTACTCTATTCAAAATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGATATACAAATTTTACTTTTGTAAAATTAACTTATAAAAATTGAAAAACTAAAAAAGAACAATATTATTACTTAAAAAATGAAGAGGTTTGAAGATTATCTTTAATCTCATCATTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAACTTATTACAAAAATGACTTTGGAATGTAAGGCAGTTGTTTATCAAAATGACTGAAAATTATATAAAAAGTAGGTTATACAAAAATTCACTGATATTTTAAATATCTGTTTTTTAATGTTGCATTTTGAAAAAAACACTTGACATTATTTAACTTTTGATATTTGTCTATTCAAAATTGAAGGAATAAGTTATTAAGACTTTGGAATGTAATGGAAAAGTCTTAATATATGAATCATTGATTCATAAAGTAATCTATTTAACTATGAAAAAGTTTAAATTATCACAAATTACGAGAATTTATTTAAAAATTTGCAAAAAATCTAACAAATCTTGATCAGAAATTAAGAATTTTAAGAATATTTATGATTTTGTGTTTGCTAAATCATCAGAAAAATATTTTTTTATCAATTTAAAACATATAAATGATTCAAAATTTAAGCAAGATTTATGGAATTTTTCGAATTCTTACAGAGTTTATGCTTGTTATCAGATTTATGAAGAAATAAAAAGCATCAGGAGACCTCTTGAACAAGTATAAATCAATTTCTCTCTGCTCTCTCATTAGCCAAGCAGCTCGGCAAGGTAATCAAAAATGGACCGAGCAAGCAAGCATGGAGCAAAATCCCAAACTAAAGTGGAATTTTTAAGCTAAATTGTCAGAGAACTACCTTCAAAAACTCTCTAAAAAATAACCTAAAAAACCACTTGATAATCAAAATGGTGCTTGTTGTTTAGTTTTAGTAGCAATAACAGGTACTATGATGGGAACCTATACAGCAACGCCGATAGTTTATGCTAGTTATCAGATTTATAAAAAATAAAACAATAGGAGAAAAATATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGATTTTTAGATAATGATTATATAAAGAATATTAGATTATTTAATAATTTTTATTATTAACAAGTAGACTTAAGACCTTATTATGGAGCTGATTTCTTTGAAATATTAGATAAAAAAGAAGATTGATTAAAGATAGAAAGTTTTATAAAAAAATTTAAAGAAAACAATCAAAATTATTTTTCGAAAATGCCAGATTTAGATCAATCAGATGAAGCACTTAGAGCATTGCTTTATTTGTAAAAGTCAGAATGAACCAAATTAAATTCAAATAAAGAAATTTATAGAAATATTAAAGAAAATATTTTCAATTTAAATGATAAAAATTCTAATTTATTCAAAGCAATTGCAAAAAGGAACAAATCTTATTAATTATGAAAGAATTAATAATTTATTTAAGTTGAAAATATGAAGGAAATATTTGAAAAATTTATGAATTTTTAAAAACTTACAAAACAGTTGCTCAAGCTAAAATAGATGAAGTATTAAATGAACTAAAACAAAAAGGCATTAAGTATATAACAGCTTTTGATGATAATTTTCCAGAAATATTAAAAAATTATCGCTATTCACCATATGTTCTTTTTTATAAAGGCAATATTAAGATTTTGAAGTTACTGGAAACTCAAAACAAAAATGAAGTTTTCATAGAATGAGATTTTGAAAATAAAAAAATTTTATTCACATCATTAAATAGTAAAGGTAAATCTAAAATCATTTACATACTAGATTGTGGATTTAATCATCTTAACAAAAAAATTAATTATAAAAAAGAGTTGTATATCACACAATTTCCATTTCAAACATTGCCTAGCAAAATTAATCAAGAATCAAGTGAAAAATTATTAAATTGTTTATTTACTGCATAAGTACAAAGGAGAAAATATGACTAAAAAAGAATACAAAGAAATTAATAATATAAAATCTAGAACTTTAAAAATGGAATTAGTAATAGATTGATTACTAAAAGCTGGTGTATTCAATAGAAGAGAAACTTATTTACTAGATACCAAAGATTTTAAAGTTGAAAAATTAATTAATGAATCTATTAAAAGAAAAACAAATTATAAAAACTGACAAGAATTATTTAAAAAAGAGCTTGTTAGATAAATATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAGTATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAGAAAGGAAAAAAATATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGTATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGATCTTTATATGGTTTTAAAGAATTAGAAGAAAGAAAATTATTTGCTGATTTACTTAATTTAAATGGCATTGGTCCTAGAGTAGCATTTAATGTTTTAAATGTTGGATTTGATAAAGCTATAAATTTAATTAGAGATGGCAACATTGAAGAGATTGCAAAAATTGAATATATTAATCCAAGAATTGCAAGAATAATTGTTGAAGAATTAAAAGAAAAATTATCATCAAAATCAAAAGACGTTAAAAAGTTAAAACTATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAAAACAGTCATTTTAGTAAAATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGAGAAGGCTTTAAAATATTCAATTTAAAAAAGTATTCTTTAAATAATCTTAATCAAATTATTAATTACAATAGGCTAAAAACTAATTTTAAATTAGGCAATAGTCTTGTTGAATATATTCTCAATAATTGTAATAATAATCCTGGAATATTAAATGAAGTGTATTTGAAAGCTATTGATTATTTTGCAATAAAGAATAAAGAAATGAATTTAAAACAATTAAAAAAATTATTAAATAAAACAAACAAAAAATATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACGAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGAGAAATTTTTCTATCTAAAAATTATGAGTCCATTTTTAAATATTGAAATTCATTAAATAGTTTAAATTTTTTAAAAAATAATAGTGTTGATTTAGTTATTACTTCACCACCCTATTTATCTTTAGTCGATTACACAAAAAGCAATTGATTAAGACTTTGATTATTAGGATTTGAAAAAAATAATTTAAAAAAAGAAATCAAACTTTCAGATTCGCTTGACTTAAAAGAATACACAAATTTTATAAAAAAATACTTAATAAATATTTCATCTAAATTAAAACCAAAAGCTAAAGTTTGTTTAGTAATTGGTGATGTTTATGATTTTGAATTAATAGAAAACATTTGAAAAGAAATTAAAAATGAAGTTCCTTATTCATTCCTAGAAGTAGGAATTGATACCAATACAAATAATTTTAAAACTTCTAAATTACTTAATTCTCGTAAGGGCAAAGCAACTAAAGTTGAAAAAATTCTAATATTAGAAAAACAAAATTAATAACTATAAAAAACAAGGAGAAAATATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGATTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAAAAGAAGGAGTTAATAAAAAATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGAATCAGAAAAAGTGATGGTAAATATCCTAAGAAAAGATATGAAATAGCTTATAGCTTATCAAAGCAATTAGAACTTATTAAATACTGTTTTGATGAAAAAATAATATTAAAAGGTGCTGTCGTTTTCATAAATGATTTTGAAAATAAAAATCCAGATAACTTTTTAATTCGAAAATATGATTCAGACAATGGAATGGACAAAAATTGAGTAAGTTGCTATTATGATAAAGATTTTTTTGAAAAATATAATTCTTTAAAATCTTGAAATGATTTAATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGAAAAGAAAATTCTGACAAAAGAAGAAAGGAAAAAAATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGAATACTAAAATATTGTGGTGAATTAAATTCTAATAAAAAATTTATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAAATATTATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGATTAGTTAATTTTTGATTCCTATTTACTTTAATTCTTTTTTGTATCATATTATTACCTATTTCTTTAGTGCTAGCAATTAATTCAGAAGATGTTTTACTATTAAAAATGTCTTCTAAAGCATTGCTGTGAACTTATACTATCATTAATGTTGTAATTACTGTTTTTGTTTTTTATAGATTTGTTTCTTTATGAATTATTGCATCTAAAATGCATAAGAAACATACATTAGATATACATAACAAAAAACTAGGTATAAAAGCAACATTATGACCATTTATTAAATATAACAAATTAATTAGCTAATTTACTAAAAACACTGTTTTATAGTATTTTTAGATTATCTTTAATTAAAGATTTATTTTAATATAAATATAAAGTATAATATTTATATAGCAAATAAGGAGCAATATGAAAACTAAAAAGATATGATTAAAGACAATATTACCAACAATAACATTACCAATTGTAACTGCTTTAACAGCAACTAGTTGTAATTGGTTTGCTTTTCCTATTAATCCACCAAAACCATCTAAAAACCCAACTATAAAAAAGGAAAATGCAGAAGGTTTTAGTGATAAAAAAGTTATTTCTGCCAAAAAATCTATAAGACCAGAAATTAAAGATTTTAAAAATATTGAGTTTTATATGGGAGATAAAAACTTAGCTAATCATGACGTAACACTATGTGCAAATGTTAAGAATTGATTAGATTTTCAAGTTCCTGGTTTCTTCTACTATGGAATATATTTAAAAACATTAATTAGTGGAAAATATTTAAAAGAAAACTTAAATGAAACAGACCTTTTATCTATTAACACTAGAAAAGAAAGATTAATTAATGAATTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGAAAGAAAGAAGATCTTTTTTTAAGTGATATTAACTTTTGCATTGAAACTTCTTTTAGTAAGAAAACTTGACCTAACTTAACAAAAGATGAATTCATAAATTTCTTTAAAGATAATGCAGTTAGATTTTATGATAGATTAAACAATAAATATGGTTACTCAATTAACTATGAATCAACTCATGGAAAATTGTCATCATTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGAAATTGAAAAGCATATACTAATATAGCTTTTCAACATAAAGCAGACAAACTTTTTGGTTCTAAAGAATTATCTAAAGATGCTCCTTTATATTGATTATTTGAAGCTCAAAATGAAGAATGAGATCATTCACAAGCAAAATTTATTGCTAATGAAGTTATGTATTATTTAAAAGAATTTCTTTTACCTTTAAGTAAATTATTAGAAGTAAACTATGATTTAGAATCTGTCATTTGAAAACAAATAGATTTCTTATATGACGTCGCTAAATTAGATGCTAGAACATTAACAAAATTTGAAACAAAAAATAAACCTGTTAATTATGCATCAGCAACTGATGAAGAATTAACAAATAAATTTAAACAACTCCAAGATACTTATAAAATTTTTGGAGATATCTCAAGCTCAGACTTGAAAATAAAATAAAAAATACAAAATAAATATTTAGTTTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAATTATGTTTAAAGACCTTTTTTGATGGCTTTTATTTAAATTATATCAAGCCCCTTGATTTATATTAATTGGTTTACCCTTATTTATTATTAATAGTTTATCAAAATTAATTAATTTTGTTTCTTTTGATTTTCTTAATTTAATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGAGTATTTACATTTTTTGCAAAATATGGCATTAATGTATTAAACACTGTAATTAGTGGTGGTCAAGAAACAAATGAACTTGGTAATTTAATTTATCAATCAGTTGCTCCTGAGCCTAGATTTATCGATCCATTTTCGATTACTTTTGAAGAATTTATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGAAAACAACAATTCTTTTCAAAAATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGAAAAAAAGAATTAGCAAGTACACAAAAACAAATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAAAAATGAATCAAAACAAAAAAATATCATCATTAAAATTAAATATTTGAAAAAATTTAAATATTTATGATGCAATAATTATTCTTGGAATTTCAGGTTTTTCAGCAGCTTTAGGCTTTGCTAATGGATTGCCTTTGTGAATAAAAATCATTATATTTTTAGTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAACCTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTTAAAATGATTTTGAAAGTCTTAGAAATATTGAAAACATAGATGTCTATTATTTAGTTATCTATGCTAATGAAATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGAGCTTGTGATTTATTTAACAGTGAATCTTTAGTTATTTGAAACTTAAAACCTATTAATGAATCCACTAAACAAAAAATACTTGATAAAGCTATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGACCTTGAATTTCTAATTCTTTAAATGATGGTAATAACTTTTTATTAGGTTTTACAACAACCTCAAGTAGTCCTGTGATTTTTGACTTATTTAAAAAGACAAAAGACAGAACATCATTTTCAATGATAATTTGAGGTGTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGAATAGAAATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGAGAAAAGAATTTTTTAACATCTGCACCTATAGGAAATGGAATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAAGCAATAATACTATAAAACAGTGTTTTTGGAGAAAGGATAGTATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGAAATGAAAGTATTAAAGATAAATGCCCTATAAAAAATGCTAACTGAGAAGAAATATTTGATATTTTAAAATCTAAATTACCATACAAAAAAGATATTGAATTGGTATCTTATTCAGGTATGAAAGATAATTTAAATTCAAAATTAAAACTAAATAAATTCAGTGGTGAAAAAACTCCTAAAGAAGAATTACTTCAATATTGAAAAAATCTTGACATTAATTTAGCACTAAATAATTATTTAAAAAACAAAAGCATTAATAAAAATGAATTTTTTAAATTTGATTTTGAAATTGTAGAAAATCAATATTTAAATATTTATTTAATTAATGAAAATGTTAATCTTAATAAAACAAGTAGATATTTATTGATTAAAAATCAAAAAATAAAGTGAAAACTAAGTTCTGATATTTCTCAGTTAAATTGAAAAGAAAGATTAATAATAAATTATGGATATAGTTACAAAATAAATAATGATGGAACAATACAAATAAAGCAATCTAAATCAAAAAATATAACTTCAAATGAAATTATTTGTGAATATCCACCAACAGAAATTTATTTTGTTTCACCACAAAATTTACAGGAAAAATTTGAAATTAGAATTGAAAGTGAAAATGGCGAAAAAATTGTTACTTTAAACAATAATGAAATTTTTTATAGTGCTATTGATATAAGGAAAATTGTTAAAAATAAATTAAATTTTTCTAATAAGCTTTTCTTAATACATGAAGATTCAAATGGCATTAAAGAAAAAATTCAAATCAAATTTGAAAAAAGTGATATATCAAACACAATTGATTTAAAATATCCGAATTGAAATCCTGATTTATTAGAATACAAACAATTTATAAGCAAAAATGAATTAAATAAAGATGGCAATGAAATTAAAGATGCTAATGGGAATCCTGTACCATCAAAAAAATATGACCCTGGGATCGATCCTAAAACAGGACTTAAATCAGAAATAGTTTGATTAAATTTTAATGAATTTCATGATTTTTTGAAAGAAAACATAAAAAACTATTGTTCTGTCTTAATAACAGAAAAAGAATCTAAATTTCTTTACGAATTCATGAATGATAAAAAATTCCGCTGAGTTGAGTTTTTAATAAATTATGAAATTTTAAAAGAAGAATTTGATCTTTTTGATAGATGAAATAATGAGGGCATTGGCCCATATCCTAATGAGGGGCGCTACATTGGAAAAAATATTCCTTATGATGAACAAGAAAAAAATATCAAAATTCTATATGAACTACGAGATTTATATGCCAGAATACAAGATTCTGGCATTGATAAATCTGAATCAATAAACATAGATAGATATTTCGAATTAAAACAAATCGTAAAAAAATATAAAAATCATTTTGATTCAACTAATTGTAGATTGCCTTTTGGTTCATTTTATTTAAAATATAAAAGTAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTGACTATTAATGCAAATTCAAATATTTTAATAAGAGAATTAAAACAAAACTCAATAGGAAAATGAGAATTTGTAAATGATTTAGAAAATATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGAATAGATAATAATAGTACTACATATACAAAACCTAGTTATTTAACAGAAGAAGCAAATTCTCCATCATTTATCAAAAAATTTTGAAGAAATAAAAATCTTAAAGTTTCAAATGATTTTGAAAAATACTTAGCCAAGAAAAAAATTATTGATTTAAACGCAACAAAAAAAGATCTTGACAAATTAAAATATGATGATATTTTTAATGAGTATAATAATTTTTTAATTGAAAAATCTTTTGAAGAAAATTTTGAAATCAAACCAGAAATTAACATTAACAAATTAAGACAAGATTTTAAAACAAATGAACAATTTTTAAATTATATAAATCAAAAGGATAATGAAGCTAAATTTATTGAAAATTATGTTATCTCAAAAAATAAAGAATTTTTAACAATTAAAAACTGTTATTTTGATTCTAAAAGTAACTTAATTAAAGTTGTTATTGATTTAAAAGAAAATGCTAGCTTAAATAGTTTTTTTATTAGAAGAAAAATCTTTAATATCAATTTAGAATTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGAAATAAAGATGAAAATATTGCAAAATTTACAATAAACTTCAATAATAACTTTAAAACTTATCATCCTTGCACACTCCATGTTATCAACAATGCTCTAGATGACGATTTTAATTGACCAGAAGCAGGCTTAACTGTAAAAGTGAATGTTGATATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGAGATTCTTTCTTTAGTAAAATTGGCAATAATGAATTAAAAATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAAAAAAAATTACTTTCATGACTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGATATAGAAAGAAGAAGAAACGTTTATAGATTAAGCCTATAGGCTTCTTTTTCTATTTCTGTGATTTTACTAAAAATACTATAAAACAGGCTTATAATAAAAATATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTAATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGAATTCAAACACTAGCAAAACAACTAAAAGGCAATTTTATTTTGTGTAAATTTCATTTTTTAAGAAAATTTAATGATTTTATTTTCAAAAAGAGAAATCGTGGATTTAACAATTTTATAGATAGCATTCAAAATTTAAGTGGGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAGTATTTATATTAATATTAATATAGAAAGGCAATTAAATTTGCTATTTTTTCCACGCAATTTTCTTTGTAATTTACATTTTTTGATATAAAATATAGTTGATAGAAAAAGGAAATACCGTTTTCTCTTTTAAATTTCTAACTTATCCCATTAATAATTTACATCACCTTTAAAAATGCTTTAAAAGCCTATAAAACAATTGAAAAAGTTAAAAATAACAATGTAGAAAATTTTTTAAAAATACATAAAATTCTTATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGATACAATAATGATATTAGTGATGTTTTAATAAAAAGTTGTGCTTTTCATTATGAATTAGAATTTATTCATCCTTTTGAAGATGGTAATGGAAGAATAGGAAGATTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTTATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATGAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGCCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTAGAACAACAAAAAGTAATAATTATTGGTAAATTAAACTCACCTAAAGTAAAATATAAATTAGTGGAAAATAATAAGGAGTAACTAATGAGCAAATTTATAATTGATAAAAGTTTTTTTGAACTTTTTCCTCAAGCTAAACTTGGAGTAGTTTTAATTAATGATATTAAAAGTAATTCAAACAGTCCAAAAGAGCTGCAAGACTATTTAAGAAAAAGTACTTGATTAGCTAAGGACCACTTAACTTCTGCTATTTTTTCAGATAATGAAGTTGTTAGAATTTATCGTGATGCATATAGTAAATTTAATTGTATGAAAAAAGCAAGATCAAGTGTTGAAATGATGCTTAAAAGAGCAAGTAAAGACGCACAATTTTCTTCAATTAACCCTTTAGTTGATATATACAATAGTGTTGGCTTACGTTTTGCATTTCCTTGTGGCGCAGAGGATATTAATAAATTTAGTGGCAATTTAATTTTAGGCATTACCAAAGGTGGAAATGAATTTTATAGAATTAATAGCACTGAAAATGAACCTGCTTTAGCAGGAGAGTTATGTTATTATGATGATAAAGGTGCTGTTTGTCGTAATTTTAATTGACAAGATGGAGAAAGAACCAAAATTGATGAAAATACTAAAAAAGCCTTTTGTGTAATTGAATTATTAGATACTAATAGATTAAATGATTTAAATGAAGCACTAAAAACATTAGCTCAATTATTTAATAAATATTTAGGAGCAAAATGTACTATTCATTTATTAGACATTAACAATTCAAAAATTGAATTAATTTAACAAATTGTTATATTTGCACTTAAAATATGCTTAAAATAAGTGTGGATATAACACTTTTTATAATTTTTTTTGTTCTACTTACACTTAAATGTGATAAAATTTAAGTGTGAAAATAACAGGAGGTAAAATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAAAAATAAATTGAAATATTTGTGTTCCTTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGAAAAAAATATTTAATCAAAAATGAATTTACTTTAAATATTACTTTTAAAAATTTAGTTTTAATGATTGTTGAATTTTTAACTGAAATTTTTGAATAAAAAAGAGTTCACCATTTAAAAGTGAGCTCTAATTTTTTTACAACTTAATTATTTAATGTTGTAGAATGCTTCACGACCTTCAAATTCATCAGCACCATCTAATTCTTCTTCAATAGCTAATAATCTATTGTATTTTGCAATTCTATCTGTACGTGACATTGATCCAGTTTTGATTTGTCCTGTGTTTAATGCAACAGCAACATCAGCTAATGTTGTGTCTTCTGTTTCACCTGAACGGTGTGAAACAACACAAGCCATTCCAGCTTTTTGAGCCATGTTAATAGCATCAATTGTTTCTGTTAATGAACCAATTTGGTTTAATTTAATTAAAATTGCATTCATTGCTTTTTCTTTAATAGCACGCGCTAATAATTTAGGGTTTGTAACTGTTAAGTCATCACCAACGATTTGAATTTGGTGCCCGTATTTAGTTGTCATTTTTGTAAAACCAGCTCAGTCATCTTCTGCTAAACCATCTTCGATTGAGATAATTGGGTATTTAGCAATTAAAGTACCATAGTATTCAATCATTTCATCTGTTGTGTATTCAAGTTTAACATTTGTAAGTTTTTCAAAACCTGGGTGTTTAGCTTCAATAGCTTTTTTAAGTTTTTTGAATACATATTTTTTGCTTTTTTCGTCGTAAATTTCACTTGAAGCAGCATCCATTGCAATAGCAACAGCATCTTTACCGCTTGTAGCTGGTTTGTAACCTGCAGCCTTAATAGCTTGAACTAAGTAGTCAAGAACTTCTTCATGACTTTTGCAGTCAGGTGCAAATCCACCTTCATCACCAACTTGTGTTCCGTGTCCTGCAGCTTTTAATAATTTTGCTAAGTTATGGAATACTTTGTTTGCCATTTGTAATGCTTCACGAATGCTTTTAGCACCAACTGGCATAATCATGAATTCTTGGAAGTCAACTGTGTTTGATGCGTGAGCTCCACCATTCATAACATTTAACATAGGTAATGGTAATACGTGGGCATTTGTACCACCAATGTATCTATATAAAGGAAGTTGTAATTCATCTGCGGCTGCTCTAGCTACTGCTAATGAAACTCCTAAGATAGCGTTAGCACCAAATTTTGATTTTGTTGCTGTGCCATCTAATTTAATCATTTTCATGTCGATTGCACGTTGATCTGTAACTTCCATTCCTAATAATTTAGGTGCAATATCTTTGTTAACATGATCAACTGCGGTCATAACACCTTTACCACCAAATCAGTTTTTTTCGTATTTGGTTCCTTGGTCTCTTAATTCAAGAGCTTCTTTTGAACCTGTACTTGCTCCTGAAGGAACATTAGCTAAACCATATCCGCCTGCTTCTGTTCATACTTCAACTTGAACTGTTGGGTTTCCACGTGAGTCTAAAATTTCACGTGAATGAATCTTGTAAATTGCTGACATTTTTCTCCTTAATTTTAAATTTTAATTTTATGTTTTATATTATACAAAAATATTTATTACTATTAACAATGAAATTTTGCTAACTATTTTTTATTATGGAAATATTACTCATAATTTTCATTAATTACAAAATGTCAATATAATTGTTAAAAATAGGAATTAACAAAATAGATAAATTTATTTTGAAAAAAATCTTAATATATATAATATTGATATTAATTAAATAGGAGAAAAATGGCGAAAAACCCAATTATAAATAAATGAAGCAAGAACTCATCTAATTATGATGACTTTATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGAGGCGATGGTAGACCATTTAACCAAGTTGAAATTGCGAGAATTTTATTTAAATTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGAAAATCTTTACTTAAGAAACCAGATGCCAATTTTGCAATCAGTCAAGATGGCTCAGGAATTAATCTTTCTGTTCGCTACAAAAAAGTTTTTAAATATTTTAAACCTGATGAAATTGCGGCTTTATATTTAAACTTTTTAATAGAAGATGATCCAATTTTTGATAAAGCAAACTTTGCTAATAGTTACATTATTAAATCTCTTGGAGCTGGCAGTTTAACAAGTTTAATAGCTTTAAAACATAATATCGGAGTAGAAGAAACTCCTAAAGTTTCTGGAATTTGAGAAATCGCTAATAGAAATAGTATTAAGAACAAAAAATTACTTTTTGCTTTTACAAGATTTAGTCAATTTGCTCCTTTTAATCGTTTTTTCAATGGCTTTGACGCTAACTTATTTATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACCAATGTTGTAATTTTAGAAAGAGAACTTATGGATGGAATTTTAGAATTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGAACATTTATTAAATGATTCCTATTTATTATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTCAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGAAATGATCTGATGATTAGCTCGCTGATTTCGACAATAGTGCAAAATGTTACACCTAAATCAATTGGTGGCGATTTAGCTACCTATTGATACTTAAGAAGAAAAGGAGTTAAACGTTCAACACTTTTACCAGTTGTTGTAACTAATACTTTCTTATGACAAGTAACTAATGTTATTATAACTGTTTTCTTCTTACCTTTTGGTATTTGATTCTATAAAGACTTCTTTACTAATATTGATAATCCACAAGTAAAAGTTTTCTATGCATTCTTTATTACAGGATTATTCATGGACTCAACTTTTGCTTCGTTCTTCTTCATTTTGGCATTTAACAAAAAAATCCAAAATTGATTATTAAAAGTATTTATTAAATTCCTTGAATGACTACCATTTGTTAAAATTTATGATCCTCTTTTAGTTAAGGCTAAATATGAATATGAATTTTATGAAATGCGGCAAGGGATGAAGAAAACATTTAAAAAATGATATCAATTCGCAGAAGTAATAATTTGAAAATTATTGCCTAACTTCTTCTCGCCAATAGCCTTCTTTATGAAAGCTTCTGGAGTATTGCAAACTAATCTAAAAGGTGGCTGATACTTTAATGTTCTTGTTTCAAATACAATTATTCGGATAGGAAACTCAGTAAGTATAACGCCTGGTGGTCAAGGCACAAATGAGATCTTTACTAATATGATTTATAAAACTATTATGAAGGATCTTAGCAATGCAGGTGAATGAGTTTACCGTGGAAAAGATTATGGACTAAATCAAATTGGTAACGCTAAATTAATGACAACTATTGGCTCAATTTGAGGAACAGTTTTAGGTTCAATTCTTTCTGCTATTTATTTGATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAGGTACAATAGATGCCTATTTTTTTGTACAAGCCTGTTTTATAGTGCTATTGGAGAAATGACGCGATTTAAAAAAGCAAAAAATTTTAAACAAATTAATAAGACAAAAAAAGTTCAAAAATTATTTTTGTAAATTTTTAAAAAATATATGTAAAAGCATTTTATTATGCTAAAATATTAAATGCTATTTATAAATATTTATAAAAGTTATATTAGTAAATAGTATGGATAGGATGCAGATGAAAGACTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAATATTACATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGAATTCAAACTCCAGTTAAAGAATTCGACAAACCATTCTTAATGGCTGTTGAAGACGTTTTCACAATCACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACTGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAATTTAATTACAATACACATAATAATATTTTGAAATTGCAGGGTAGTTTACCCTGCTTTTTTATATATCTAATCAAATCTTTTTGAAACTTTTAATATATAATTAAAAATAATATTTAAGGTACATATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGTAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAGCATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCATATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTGTTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCTTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCAGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACACTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGTTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGATAATCATCCAGAAATTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAGATTAAATGAAATTAATGATTAATAAACATTTTTTTGCATCATCTAAACTTTTACAGTCTCTTAATCCTTTTTCTGCCCCCCCTTTTTTTTAATTAAATCAAGAGAGACGGGAGAGGGATTAAGATTTCTTGCATAGCAATAACTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTTCACAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTCTATTAAGAAATAGAGAGACTTTACACAAAATTCTAAACATTCTCTAATTTAACTCCATTACCATCATTTACTTCATTAAATTCTTTTGCGAAATTTTCAGCACTAAAAGTAGTGGTGTCATTGTTGTACTTTTCATTTATAAATTTTATAAATGCATCATATATTTTTTCAATATGTTCATCAAGGGTTAATGGAGAGATTGAATGGGCCACAGCTAGAATTTTATATTTTATTTTTAATTTGTAAAGTATTTGTAAATCTTTATTTATTTGTTTTACTATATTTCTTTTAATATTTTTTATTTTTTTAATATCGGTTTCTTGATTTTGTTCAAAGTACTTAAAGAAATTATGTGCTACAGCAGGACTATCTTTAATTTCAAAAATATTTTTATTTTTAAATACAAAAAAGTCTTTAATACCAGGTCAAATATCATTAATGTTAATTATTTTTTCAAAATCAGCAGCATGCATTTTGGTATTTCCAAAACTTATTTTATATTTGTTATTATTATCTACACTTAGAGTTGCATCTTCAAAATAACGCGGTTCTTTAAAGATAAGTTTTGGCAATTCTTTTTTAATTTTTTCATCTATAAAATCTTTTAATTTTATATAAGGCCAAGTATTGCTTTTAACACTTTCATCTTTAACATAATTAATTATAGAATCTTTGATTTTTTTAATGTCATTATATAAATAATATTCTTTTCATTGATTTTTTCATGAATCAGTTGTTTTCATACGTTCTGAGTATAAAAGTATTTCATTTTTTCTTTTTTCAATATAATCGTTGATTGCTAAGTCAAGCAACGTTTCACATTCATTATCTACATTATCAAAAATAATTTCTGATTTCTTTGTGATTGCATATAACACATTGTTGTAATTATTGTTTTCAATTTCGTCCCAATTGAAATATTTTTTTGAAAAATGTCTAAATTTCTTGATTTTTAAAGTAATTTGTGGTTTATTATTTTGATTTGAAACTTCAAATTCAAAGTTTTCGTTTGATAAAGTAGATTCAAATATTGATTTATTCAAGAATTCATTATCAAACAAGAAATTATATATTTTGTCTAAATTTTTTATTATATCTTCGTTAAATAGTAAATGAGAAATACTTTCTTCACTTCATTCCGAATTTTCAAACATTATAAATTTAGAGTTTTTTACATTTTTAATGACATCAAGACTATTAACATCAAATATGAATTGATTGCCACCTTCAAGTAAATTTTTTATTATTTCATATAATGATTTTTTGCCTTCATTAAAATCCTTGAATTCATCTTTAAAAATTCTTTTTAATCTGTTAATCTCTTTTTCGTTTTTGTCTGCAAATAATAATCCATCTTGTAAAATTTGTCTTATTAATCATTGTCATTTAGTAATTTTTTCATCATATTTTAATTCTTTAATTCCAGTTTTTTCACCATTAGAACGAATTTTTCTTTCATTTAAAGAAATCAAGAAAAATCTTTCAAATTCTTTTTCATGAGTATTTGGATCTTCTGCTCCTGTATCATTTAATTTAATATCTTCAACTCTTATAATTTTTTCATCATTTTTTTCATAAGTTGATATATTAGTTTCACTTGATAGATTTGGTACTGTATTACTAATATTTTTAGCCAAATGATTTTTCTTAATCACGTTTAACACATAATAATTTTCTAAATTGTCTATTTTGCTTTGTAATTGGGAAACAAATACATTTCAATCTCCGGCACATTCAAAATAGTTAATACTTTCATATTGATTGTTAACACTATTAAAATATTGGAATTCTTTATATTTTTTATTCACTATTCGTTTATTACCCGAATTATTATTGTAATAATCAATTAAATTTGAAAGTTCAAGCTGAGTGTCTTCATTAGCAATTTGTGTATCGCAAATATATTTTACAAATTTTTCAGAAACTTCATTTGTTGAATTAATTTCGAATAATCCAAAATGTTTGTTTTCATAATTTGATTTGAAAAATAAAGTTAATAATTTTTTTAAGTTTTGAACATCAGTGAACTTAAATTCCTTTGTGTTATTGTCATCGATTTTTAATTTTAAACTTTTTTCCTTTATACTTCATTGAACACTAGGGAAGCTTTGTTTATTAAGAATTATTTTAAAGTTTTGAATTAAATTGTAATCTTTAAATAAATTTTCTAAATCTTTATCAAAGTTCTTTTGTTGATTACCATTAAATAGATTTCTTGCATTAATCAAAGTATATAAATTGTTTTTTATAGTTTTTCATTCGTTTATAGATAGTTGATTACCATCATAAGATATGTCACCTATAGTAGAATCTCCTGTTAAAAATTCTAGCGATTTAACAATTTTTACTTTTTTTCCTCGATAATTTTCATTTGCAAAGATATCACTTAAAGAGATGTTAGCTGTTTTTGAACTATTTGCTATTTTTTCTTTAATTCTGTTGTAAATTTCAATTATAATTTCGTCTTTATTTGTTGCTGTAACTCATTTATCATATTTATATTCTAAAAATCAAATCGAACTATTTAAATAATTTTTTAATTCGTTATATTTTTTAAAACTATCAAAGTCAGAAACACCTGATGGTTTTAATGTTTTTGATTCAAGAGTTACAATTTCTATATTTTTATCTTTGTATTCTGAAGGATAATTTACACCAGCTTTTATATTCAATTTTGATAAATCACATTCATTATCAATTTCAGAAATATAAGCATTAGTCAATTTACTTTGAATATCATTTTTTATTTTTTCAATTATTTCTTTTCTACATTTTTCATAATCTAAATCTTGTAAAGGATATTTAAAGATATTATCTTTTTCTTTAAATAATTCATCAACTATTTTGTTGATATTGTTTGTAGCAAAAAAACCTATTGTATTAAATTCATAAATTTTGTTTACAATATCATATGTTACGCTTATTTCTTTATTTAAATTATTAATGTCGAGATATGTTGTATTATCTTTTTTAAAACCAATATTTTTTAATGAAATATTAAATGTATATTTATCATTTATAAATGAATCAAAAAAATTTGAATTAGTACCGTAAAAGTAATTTGTTATTTTATCGAATTTCTTATCAACTGATAATTTTGCAACTTCTGAATCATAATAAAATGTTTTTTTGTTGTAATCCGCATTTGAAAGTATATTTTCAAAACGACTTATGAATTCTAATTTTGAATTTGAAATGTATTTTTTAATAAAAGTTTGAAATACAATATTTATATTTTTTTCATCTTTTATTCCACAATATTCCTTAACTATATTTAAAGTATTGGCATTGTTTAAATCCGGCATTATGATAAAGTAATTATCGGTCAATTTGAATAATTTTGCAGAAATTCAACTTGTAATAACATTGTTATTTTGATTAGTTATAACACTTAAAGGATCTATGTTATTAAATTCATATTTTCATATAGGCATTTTAAGTTGATTTTTTATTTTATTTCAATTAGTTGCTTTATTTGAATCTACAAAGAATAGACCAAAACTATTTAGGTTACAAATAAAATCACTAAAATAATTATCATCTTTTTGAAGTTTATTTGTTAAGTATCCTAGTTTGTCATAATAATTTAATTTAAATTCTTCATTATTTTTTTTAAATATTTCTTTTGTAAATGGTGTTTGACCATTAAAGTTAATACTTAAAAATTTTGCAAAAGACAATCTAATAGTTTTTGTTGGAATATCTACAATGTAATCAACAATTTTGTAATTTACATTATCTAAATTTGAAGTGTAATATCCAACATTACTGGTTTGTCCTTCGTATTGTGATTTTATAATATTTTTTATATCTGAATATAAAGCATTATTTGCAAATAGTAGATCATTTAATTGAGCAATTGCATTTGAACGACCACCATTGTTTGCTCATTCTGTTAACTGTTTAACAGTAATATCAACTCTGTTTTTAATTCTGATTTCTTGATTGTTTTTTCCATTAATCCCTAATTCATAGATACTTTTTTCATTATCTCTTCCATAAAAATTAATAACTATTTTTTCATATTTTGTATTGTTGTCTTTTGGATTTGAAAAGCTTAAATTAGCATTATATTTTTTAATGTATTCTTTTAATTCATCATTTTTATAAAATGTAATGTAATTTTTAACACTATCTTCAAATCATTCCTTACTTTTATCAATTTCTAAAGCCATTGCTAATTTTTTAAGATCCTCATCATCAAGTGTTCATTCATTATCAACAATTTTTCTTTTGACTTTAAATGTTTTATCAACTACTTTAATTTCAAAGTTATATAAATTAGTGTTTGTGTATAAGCGGATTTCGCCTTCTTTTGAAGACTTTCAATATTCTTTAAACAAATTATAAACAAGGTCATTTTTATCTACTTTTTCAGGTACTTTGTATAAAGTTTTTTGGTCTTCAGAAATGAAATATAAGTTAATCAATGGTGTATTTGGATTAGAAGAAATTAGTTTAGTATTATATTTTTTATTTTCTGAGTAAGATTCAATAAACTCATCACAACCTGGTTCACCTTTAATAGTACTAAATTTTTCAGGAGGTAATAATTCATTTTTAATATTATTTGAAAATATTTTTCTATTGAAAATGTCATCTAATTTATTATTCTTTAATAATGACTCAACTTCTTCGGCATGTTCTTTATTAGTTTTATCGATTGAAATATCAAAAATCTTAATTCTTGGAGAAATATCATATCTAATTTTAAAAGTATTGTTGGTATTTTTTAATTCAGCTTTATTTGATTCATTGCCAAAAACTGTCGAATTATTAAAATCAGAATTTTTTAATAGTTCACTACAATAAAATGGATAATCAATGTTTGTTTTTTTATTGTTTTCCATTTTAAATGTTTCTTCTTTAATAAAACCTTTAGAAATAAATAATTGTTTTAAATTAATTTCTCAAATGTCATTTTTTATTTTTTCAACTATTGATTTATCATTTGAACTTATATAAATGTTGTTTATTTTTAAAATAACCACATCATATTCTGAATTGCATTGACCTTTAACAAGCTCAATTCTAGGCATCTTTTCTCCAAAGTTTCTTAGTTTAGCTTTGAATCTAGTTTCAATTTCATTTTTTATTGTTGAAAAATTAATTTTTGACTCATCGCTTTCATTTTCTCAATCAATCATTCCGCTTCTAAAGTAAAAATGCAAGTCAAAGTTAACTTGTCTACTTTCTCTAGGCATAAAGTTCAACATAATAGTTTTATTACCTGCAAACTTATCGCTATAGGTAATAAATTTGTTGTTAATTTTTGCACCATATTCATCTATTCTTCAGTCTTCATTTTGGCAAATTTCATTAATAAATTTGTTGTCTTTAAGTAAAGACAATACTTGTTCTCAAGTGCTTCCTGATTTAACTTTTATTGAATCAATGAACACATCTTCATTATTTTTGTTTCTATATTTTAGAGTTAGTTTTGTTCAGCTATTTACATTGTAAAAAGCAGCAGCACTAACTACTCCAAGTGTTGTTAACGAGAGTGCAGTTGCCACTGTCGCTATAACTTTTTTTCTTTTGTTTTTAATCATAATTTCTCCTAAAAATAATTCATATATAAATTATATACTATAATTTTTTCGTTTTTATTAGATATTATTAAGTATTAAATTTGAAAAATATTTATATTTTTCTAATTCAATTGTTTTATAGTTGTTTTTATTTAAAAAATATTTAAAAATATATAGAAAAAGATTTTTTAATGATTTTGTATTAAAAAAACTAAAATTTGAAGCCATTTTTTTACTAAAAAATCTGTTTTATAGCCTTTTTGGTTAAAAATAAAAACAACCAAATATTTGTCGCAAAAATAAATAATTTATATTAATATATATTTTAATATAATATAATCATATTTAATATATTAAATGGAGGAATAAAACATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGAAACGAAGGTGAGGCTAAATATGATATTCGTGACTGAAGTGAAGATCACTTACGTTCAAGCAAGGGCATTACTCTTAGTTTAGAAGAATTAAAACAATTAAAAGTTTTGATTGACAAAGAGCTAGTAAGAATTAAATAATTATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAATAAAGGAGATAATATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGAGACTTAAAGAAAATTGCTAATCCAAGTGAAATATTCTTTGTAGCTGATGCTTTAAGTGGACAAGATATTTTAAATGTTGCTGAAACTTTTAATAATAAATTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAATGCATAAAACTGTTGACCATCACCACCCTAAAGGTTTCTTTTCCTATAGTGGAAATCATTTGAATTTCACAGGCTTTTCACAGGCATTCTTTTATATAATAGCTTTTTTGTCTATAACTTGTCTTTTACTAATTTGACTGTTTCATAGACAAATTAAACAAAAGTACTTAGTTAAAATTAAATTATTTTATTTACAAAAAAGAACATTTTGACTATTATTTGGCTTGATTATTTTATTAGGAATGGTAATTCATATTATTATATTAGCTGCTGATAAATTTCATCGAGCTTGAGAATATTTGCCTTTTCATTTTTGCCGAATTTTGATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGATATTATTGAGATTACTTGTTTGCACATGTTTTTGTATTATTGATGCCCGCAGTAATGTATGCTCTCAGCAATAACAAAATTAGATTTAAAGATTCAATTTTTACAGTTATTTTCTTTTCAACACTTTCATTAACAATGTTTTTAATTAACTGAATAACTTATACATATAGTAAAAAATTAACTTGAAAAACAATGAATTACTTTTATTTAGGGCCTAATGAATATAATGGTTTTAGAGACTTTATGGGACCGCTTTCAAAATGACCTTACAGTCTTTTTACTTATATATTTCTCGGCATTTTAGCAGTTATTATCTCAACAATTTTTTACTGTTTTCAAGACAAAATTCATTTGGCTAAAGTTGAAAATAAACGAGTTTTAAAATGAATCAAAAGTGAAAATTGAAAAATATATCGCTCTTCATTTAATACAAGAAACCAAAAAAATGATGAATTAAATTCTAGTTCTAAAGACTTAGAAAACTGTCAAGATAATAATGATTCTATTGAAAATAATAAAGAAAATATTAGTCAAACAGTTGACTAAAAATTAATTAAAAAAGAATCAATTTTGAGCTCAAAAAAATCTCTTAAAAAAATTGATTCTTAAAAAATGAATGTAATATAATACTATTTATGTTAGTAGTTTATGACATGCTTTTTGTGTACTTTTAAAGAAAAATACACTGGAGATCTTATGGATAACCAAAAAGAAAAAAGAGTGCATATAATCTTTTGAAGTTTAACTTTGATTTTAGGTTTATTATCACTTGCTTTTTTTAATTATAAAAAAACTAAATTTGCTGTAATGCTTGGCTATTGAATTGGCACAAGTTGTGGCTTTTTAGCATACGAACTAAGAGAGTTATTATCTTTTAAAATTATACAAACTAGTAAAAATAGAGCAATTATGCTTGGTTTCTTTGGAACAATATTAAATATGCTTTGAATTGGATTATTGCTTTTTGCAATTTTCAAAATTAATTCAAATGCGCTTAATAGTCCTTACACAAAAACCATTGAAAAAAATAGTTTTTGACCAATAAACATCATTGCTTTTATTCCTGGTTTTATGACATTCTATTTTTCTCTTTTCATTGGATATTTAAGTTCTGAAAAAGACAAAAAAGTTCAAAATAACTAGAAAAGGAGGTCAAGTGGAAAAACTGACACAAAACTTGTGAAAATGAAATATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGAATCTGAACACAAATTCCTGGCCTACTAGGTGAATGATTCTTTATTGGATCATTAATTACACCATTTTTACATATGTATTTTGATATGTTTGGTGCAATAATTCAAGCTTTAGTTTTCTGTACATTAACAACAGTTTACTGATCAAAAGAAGCTGTAACAAATGAAAACAAGAAACAACAAGATGCTCAAGAAAAAGCAGAATCTGAAAAATCAGTTGCTAATGTTGAAAACAAAGCTGTTGCTTGATCAAATTCAAGCAGCGTATATTAAAAATTAGGAGGTATTTATTATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAATTTAATAATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAATTTTTCGTATTTATATATAAAACAATTAATTTAGTAAAAATATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGAATGGCCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAGGAGGTAAAAATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAATAAAATTTGCTAAAACAACTGTTTCATAGTTATATAAGAAAAAATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAATTTAGAAATTAATGGACCATTTCACAAGGTTCATTATTTTTTATTTTTTTGTTTCTTAAAATATATTTTTATACTAAAAAAATCATTGCTTTAATGAATAAAGTGCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCATATTGAAAGCACAGGTTTGATACAATGAAAACAACGTATCAAGCCTTTTTTTGTTGCTTTTTATAAGCTTTGTACGGCCTTTTTATTAAAAATAGGTATCATTTATCCTGGTTTCCACTAAAAACAAAACAGTGGGCCAGGATTTTTTATTTATTTGACATTCTTACAAAAAAATGAAAGGCCGCTATAAAAAAACGATAGGTTCTTCATATTCGTTAAGTGTTTTTACAATTTTTTTACTTTTAATATAACTGTGAAACGATAGATTTTCATAATAAAAATTATTCGAGAAAAATATGGAAATATTCAGATATTGAAGAAAGCGTTCTACAATAGCGAGAAGATCCTCAGGGTGGTTTCCTTGGCTTCGTTGCTTATCGGATCAGTAACAGGTGTCGTTTGCTTTTACTTTATCCAGTCGATTATACCAGTTGCAAATGTTGCATTGGACATTGAAAGTTATAAAAATTATCAAAAATGTTGTTTTTGGGCAAAATAGTGATATGATAATAGCATAAAGAAGCTTACTTTTAGTATAAATTTTGTTGAAATTTTGACTTATTTTTAGTGCTTCTGTTATAAAAATCACTACCCAATATTTTAGCTTTTTTATTAAACTATTGAATTTTTTTACCTTTTATAAATACGGAGGAAGCCTTATGGCGATAGAAACTAAAGTAATTAACACAAGTATGTATGGAGTATTTACCCAACAAGAAATAATCGACCATTACACTTGTTTTGGATGACAAACTATAAGTGTTACCGATACCAGAGTAACTATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGAAAAGCAAGCATAATCAATGTTAAATTATTAATTATTTTGTTACTTTTATTCATTGTGGGTGGAGTATTTTACATACTTAACAAAATGAAATGAAGAAAAATTTATAGAAGAGAATCAAATGCTATGCTTGATGCAGTTGAAAAAGCTAGAACATTATATTATGCATATAATTAATGTTTAATTTATAGAACTACACAAGTAATACTGCTTTATGTGCTGCCTATAAGGCCGCACGTTTTTATTTAATGCTATAAGAAACGAGATGTAATTATATTATTTCCAACGATGCTAAGCAATATATATTTTGATAGTGGTATAGTCCTCATTGAACATATATTCATAGTTTGAATCTCTTAAAAATTCTTCAATATATGCTTTTATAGTTTCAGTTCCTTTGCCGATAATGGCTAATATGTAATTATTGCTAAATTCTGAATTATCTTTAGCATTAGCAATTGCAAATTGAATTTCTGCTACTGCTTCATAAATATTTAACCCATGTAAGTCGATGACATTTGATTCTGCTTTATCATAATTGATTATTTTTTTATAGTTTCTCTTATTTTTTCTACTCATAATTTTCTTTCTGGCAATTTGGACAATAATAAGTCACTCTTTGAGGTAATACCGCTTGTTCAATTTTCTCGCCACATACTTCACATGGTTTATTAGTTTTTAAATACACTTTTAAAAAGTTCTGGAATTGGCCTTCAACCGCATTCAGTGATGAATAATCGTTAATTGTAGAACCACCTAATTCAGTAGACTTATCAAGAATTTGCTTAGCATTATTTATTATTTCTTTTCACTTAAATAATGGATTTTATTCGCTTTTGTTCAAGGGCTAATTTTTGATTCAAAAAGAATTTCATTAGCGTGTATATTGCCTATTCCTAAAATGTAAGACTGATCTAATAAAAAAAATTTTATTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTCTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAATAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGAAACAGATGTTACCGAATTTAAATTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATAAATTTTAGGACCAGAGAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAGAAATGTTAGAAAATGTAGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATATTTGGGGTTCATACCATTCATACCAAGGGTCTCACTTTTGAAATTAACAATTTATAGAATTAACATTTTTTTGTTTCTTAAAATATATTTTTATACTATAATATCAATACTTTAATGAATAAAGTGCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCATTTGATTAAATTCACCGTTTCTTCCTTATTGGAAGATTTTTTATTATAAAAAATAGCCTAAGCTAGGCTACTTATAAATTTTTAATCAACCATTTTGAATCAAGGAACATTTACTTCTTTGATTTCTAAAGGACAAAGCAATTTTGTTTGTTTGTTTTTATTAACATTATCTGTAATTAAAACAATAACATCACTACAAATTTTACTATTTCTTAATTTCTTAAGATCAACAGTTGCTAAGAGTTGTCCTCTTTCAACTTTATCATTTTGTTTAAGATTTGTTTCAAAGCCTAAACCATTTAATTTAACTGTATCAATACCAATATGTACTAAAACTTCAAGACCATTATCATTTGCTATACCATATGCATGTTTAGTTGGATATGACATAATTAATTTTCCATTAATTGGTGCATATACTTCTAATTTGTTTAATTTTTTATCAATTTCAATTGCATAACCACAACCTAGCAAACTAAAGGTTTGATCACTTAAATCTTCAATTGATTTTCTTGTACCTTTGCAAGGTGTATAAACTATATTTTCATCAACAGTTTTTTTAACTTCAACCGGTTTTTCTTCAAATAAGTTGACATCACTTTTAACTTCAATTTTTTTATTTTTAAGTGCTTCTTCTTCTAAGAATGGTGCAGCTTCTTGAATATCTAAATTTAATTTTTCACTAATTTCACCAAATTTAGCTTGCACATGTCTTTTTCCAATAAAAATAACTTCACTTGCCCCAACTTTTTTAAGAGCTTCTTCATTTACTAATGAAGTATCTTTAACATCATATCTAAGACGAACAGCACAGTTATTAAAACCTGTAATATTTTCAATTCCACCCATAGCATTAACTATGTCTCTTGTAAGTTGTGAAATTTCAACAAGCTTCCTAACTTCCTTAATTTTTTGTTTATTGTCTTTACGACCAAAAACTCTTTTAATTCAACTAACAAATCCCATTTTGAATTATCTCCTTGAAAATTAAATCTTATACGTAAGATATACTTAATTAATATTAATATAAAAGTTATCTTTTGAGATTATCTTTAGATTAATAAATTAATATTATATGAAAACATTAACATTAATAACAATAATAATTTGAAAAACATGAATTTAAAAATTAAGTTGTTTCATATAATTAATAAATAAATTGATTAAGTTATATTAATTTAATTAATATGTTTTGTTAGGAGAAAAAATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGCCGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGATTACAAAGATCAATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGAGAAGAATTAATTAGCAATGAAAATGTTATTATTACTTCTCACCAAGCATTCTTAACAGATGTTGCTTTAACTCAAATTGCTAAAATCACTTTAGAAAATGCTGATATGGCACAAAATAAGGACTTTTCTAAAGCATTAGTAATAATGGAAAATGGAAAAGTTAAAAACGGATAGAAAATAATCCTAATAAATAAAATAAAATTTGTATAAGCCTGTTTTATAGGCTTTTTTGTAAAACTTATTTATTGAAAAAGGAGAATATATGTTTAATTATTTTAATTTAAAAAATCGTCAGGCTGCAGTGCAGCCTACTAAAGCGATTCACTGATTTAGTCACTTATTTAGTGAACTAATTGGAACGATTTGAATTTCACTTGGTTTAGCAGGTTTATCAATTATTATTAAAGATAAAGTTGTTGAACATTACTTATTACACAATGTTATTGTTGGCTTTTTTGCTGGCTTTGTAGTTGTCGGATCATGCTTAATTTTCTTTGGCAGATGATCAGTTGATTTAAACCCAGTAGTTACACTTTATCGTTGATTTAATGGTACTAATAAAACTGGTTATGTTATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGAAGTGGTGGTGTTTGAATCTTCTTTGGTGAAATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGAATGGGTATTCTGCTAGGTTCAGCTGCCATTAACCCTGTTCGTGGTTTAGCTCAACAAGTACCTACCTTATTCTTTGATATGAAACACTTAAGTGCTATGGCTCAATATGACATTCACATGGGTACTTGAGCAATGGTTTTAGGTAACTTAATGTCACCAGTCTTCCATGCATTCATGCAAGGATTCACTGAAAAATATGGTAATCCAGCTTTACTTTGAATGTTAAATTACAAAAATAATAAAGCTAGTCATTATGTAAAAGAAACACAAACAAAAGAAGAAAGCGTTAAAAAAAACAATTAATTTCAAGATAAACTTAACAACCTAGTTATGTTTATTTTTTATTATTTATGCTTCTATATAATAAAAAACAAACAACAATATACCTTCTTTATTTATATACAATAGAGCCTGCATCTGTGTGAAAAATTTTTCAAAAAGTTTTTGCATTCGAAAAAAAGCGTATATAATTATTGCGTTATGTTAAAAAATTGAAACTTTATTCGTATTGAAAAGAATATCAACATTATCAGAACAATTATGATGATGTCTAATTTCTAATTCAATACCTCTTAAAGATCAAAATGTAATATAAATTTTGTAGTTTTTAAGCAGGTATGCAACAGAAACCTGCTTTTATAATAGACGCTATAGAGGAGCTCTTAGTAATAGTATATTAGTTTAGCAGGCATGTGTTGAGACTATATAAAAAGGATATAAGAGCCGAACTGGTGATTGTGGCGGCAATTACTGGTGGTAACAATGCATAACAGTTTGTTACTCTTTGGACCTCAAACCATTGGTGCTATAGCTTTGCAAAAATAAATAATTTCTTTAATTATTATTTTGTAGAGTATTTAGTGCTTTACAAAATTTTTATTTAATCATAACATTAATTATTATTAAAAAAGATATTAATATATCGAAAGGAAATTATGAATAAAAAAAGAAGATTATTATGATCTTTTGCCCCATTAGCTACATTAGCTGCTTTTGCGCCTGTTGCAATCAGTGCTAGTTGTGGAAGAAAAGGCCGTGTAATACCTAATCTTGACTTAATTAAAGGGCAAACTAACTGAAGTGTTGTAAACTCTAAATATACTGAAGATTCATACACTACAGACCTTTCAACAACTTATGGTGGTTTCTTAAATAGATTAGAAGAAGAAACAGGAGCTTATTTACTTACAACAAGAAGTTTCGGAAATTGCAAAATTGTTCAAGCTGGTCCAAAAGGGCATGAGCGTAAATTTGTAAAAAGTCCTTCATATTGAAGATACTCATTAGAATATGCAGATGCTGTTGTTGTTACAAAAGCAGATGGTACAGAAGCTGTATTTGACTCAGATGAAGCTGAATTAAAACAAACTCCAGAACATCCTGAAGATAAAGCAAAAAATATACCTGCACACTTTGATTTAGCTAACTATCATGCTAGATCAGCAAATGCTAGAAGTGTTAATAGTGCTGAATTTGAAAAAGCTCTTAAAGGAGCTAAAAAAATCCAATTGCGTGTAAGAAAAGGTGCTAAATGAGTTGATTCATTAGGCAATGAAACAAAATATGATGTTGTTGGTGATGACTTCTACATCTCATGACTTAGAACTTACACACTTTCAACTGGTGTACGTAATAGTTACTTAAAAGGTACAAAATATGAAGACAAAGTAGCAGAACTTGATAAACTTGCAAGCGGAATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGAAATGGTAAAAATGGAAGTAAAAGTAAATCAAACTTATTAAGTGCTTTATCTGAGCTTCCAGAAGATAACTTATTAAGAGTTGCTGGTGTATATACTTATGGTGCTAGTGAAAAAGACATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTTCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGAAATGCTGTTGCTAACACATTATCAAACAATAAAACTAAACCTTGACTTGCTGGTGTTGCGCCTCATGGTAAAATTGGCGGTTCAGATCAAGATACAACAGCTGCAGCGAAAAGACCTGTTGACGATTATGAAAACTTAAACACATTCTACACAGTTGATTCTAATGGTGAAATTGGTATAACAGTTACACCTACAGAAAATGCTACAATTGCAAACACTGCAAGTACAACTGAAGAAAAATACAAATCAGCTAAATTTAATGAAGTTAAAGCTGCTATTAAAAAAATTCTAGATGATTATGAAGCTACATTGCCAGCTGGTGAAAAATTAGAAGTTGAATGAACTGTTCCAAATAGATATATAAACTGAAATCCAAGTAACATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGAGGTGATTCATTATTTGGTGGATCAATTGCCAAAATTATTAGTTGAGGTTATGATATCGATAACATCGGTTCAGGTATTGATGGTATTACAAACAGTGGATTTATCGGAGCATTATTCTCAATTGGTACAAGTGCAAAATTACAAAACAAATTACAAAAAGCTTTTCCAACATTAGTTCAAGCTTCAAAAGAACTTGTTGAATTTGCTTTAAAATCAGAAAATAATTTTAAATTTGGTTTTAACTTACATGACTTCAATAAAATACCAACAAAATTTTCAAATGATTTAGCTGAAAACCTTCACCACTTAAAATGAGATTCTAAAGAAAACAAATTTGTTTTTGACGAAAAAGAAAACTTCACAATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTAACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACTTTAATTAATAGTGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGATTAGCAGATATAATTGTTAAAAATGTAAAAAAATAGTGTAAATATATATTTACAAAAAAACTATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGAACTATTTTAATTTCATTACCAGCTTTCGTTATTAGTGCCATTTTAGGTGTGGCAATTGGCGTTTTAGCTGGATATAAAAGAGGAACAATTTGAGATACGTTGATAAACATTTTCATTTTTATCTTCGTGGCTGTTCCTTCATTTGTTTTAGCTCCATTCTTCCTTAATATCTTTAACAAATTAGGATTTGGCCGAACATTTGTTTCACCATATGAAACAGATAGTTCATGAGCTAAAACAATTAAATCAATTATTCCTCCAATTACATTATTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTCTATTAAGTGGAAGTATTATTATTGAAAGATTCTGATTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGATTTGAAGTTATTAGAGCTTCAAGATTCCGTAGAAAAGCAGAAAAAGAACTATTCAAGCAACAAGATCAAGAATTATTAATGAAAGGAGCGAACTAAAACATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGAGAAGGCTATCATTATATTGAGCCTTACTTAAAAGATGTCATAGTTAATAGTAAAGAAAAATTACTTATATATAATGCCTATGATTTTTGAAAAGCTTATTATATTTGATCACGTGCTTTAAAAATTAATCCTTTAAAACCAGAAGATATAGAAAACATCAAAGCTTCAATGCCACATTTAAATACTCTTTTTGGTACAACAGTTAAAGAAGCAGATATTTGAACAGGCGCTTGATTTGGTGCAATTAGCTCAATTAAAGTTGCTTTAATTGTTGCAACTATTGAGGTTGTAATTGGTGTTGCTGTTGGTGCTATTCTTGGTTTCCATGCTGGAAAATGACTTGACACTGTAATGATGAGGGTCATTGAAATTTTCCAAGCACCACCTTCAGTTATTTGATTATTGCTATTCATTTCTGCTGCTGGAACTAGTGAATGAGTCTTGATTGCTGGATTGCTTTTTGTTGGTTGAACTTGACCTATTGGTGCAACTAGAATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGATTAATGTGACTTCCAACTCTTATATTATTGACACTTTCAGTTAGCTTACAATTTATTGCTATTGGTCTTCACGATGCTTTAGACCCAAGAGTTATTAAAGTAAAAGGATAAAATTATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAATTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATATTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGACAATCAATTAGAGGAACATATATTGGATATATCCACCAAGATCCTCTCACAAGCTTAAACCCTACTAGAAAAATAGGTCTTCAATTATTGGATGCACTTAATCTAAATAAAGAATGAAAAGGCAAGAGCTACAAAGACAAAAGAGCCTATTTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCGTCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGAGCTTTAATTTCTGCTATTCCAGAAAACTCACAATCAAGACTTTACTCAATCAAAGGAACACCACCTGATATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGATTATTACATCCAGATAGTCCAAAAGTAGAAAGACCAGAAGAACTTGAATATAGACTAAAAAGCTTTAGAAAGGTATTTAAAGATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGATTTGATATTAAAGAAAACTTCTTTTATGTATTTAAACAAAGAGCTTTAGAATTAAAGCTAAAAAATCTAGAATCAATTAATCTTTTAGCTGAAAAATTCTTTCCATATTGAATTGAAAAATTCAAGAAGATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCACAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGAGTTAAACCATTTAATATCAACAGTTTTTCATTAGCAACAAACAAATCAATTGATGAAGAATACAATTCAAAAATAGATCATAAACCATACGATGGACATAGCGAGATCCCAAATAATATCGAATATACAGATGAAATTAAAATAGACGAACCTACATGTGAAATAAAAATATCTTCAAATTTTGACTTCTCTAAACCTATAGTTGAAGTTGTAGAAACTAAAGTTTCAACAACCAAAACAACTAAAAAAAGAAGTACTTCTAGAAAAGCTTCAAGTGCTAAAAAAACAACAAAGAAATAATTTTTGTTAAAATTTCATTACTATGAATTCAACAAGAGAATATTTTAGAGAAGCTTTTACTTGAAAAAAACTTCTGCACTTATTTATTATTTTATTAATATCTTTAATAGCAGGTGTTTCACTTTATTTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAAAAATCTAAAATGCACTAAGTGTGCATTTTTTGTTTATAAATAAAAAACAAAAATATAATATAATTAAAATAACTTTTTGAAAGGAGATAAAATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGATGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGAAAAATATTTAATCAAGCTGCAACAAATGCCAAGGAAAAAGTATGAAGAATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAATATATAGAAGACTTTTTCTTAATTGAATTAGTCTTTTTTATATGTTTTTTCAAAATTTCTATTGAAAAAAAGTGGTCAAATGACCATAAATTTTCAATAGAAATAATCAAAAATAGTAAAAAGTGAAAATTCTACTGAAAAAAAATGGTCAAATGACCAGAAATTTTCAATAGAAAATAATAATTTAGTATAATTTACATGAGGTTTTTATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACTGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGAAAAGAATATTGTGAATTTGGTGGTATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGTCTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGATGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGACTTTTTATTAAATGATAACATTTTTTAAAAAAGCTTATAAAACAATTTAATTAGTCAAAATTAAAATTGCAGATTTTACTGTCTGCAATTTTAAATTCTTGTTTGATAATTATTTTTATCATAGTTAATTTTTGCAGTCATTTTTTCTCCTTTTTTATATAAAAAATTATAAATTAAAATTTTGATTTTTGGAAAAAATGGTATTTTGAAAATAATAAAAAACAAAAAGCACTATAAAATAGTACTTTAAGTAAAATGTATGTGATTAAAAATTATTGTATTTTTTAAACACACTGTAAACTGCATCAACTGCTAAATTGAATGCTGTTTTGTGACTAAAATTATCAATATCAGGATTCTTTTCTGATTTCTTTAAAAACAATTCTTTTGTTTCTTTTCAAATTCTGTCATATTTTTTAATTTCAGCAACTTTATCACCAGTATATTCATAGTCATAAACCTTTGTTCAATTGTAAACTATTTTATTAGCTTTATCTTTAGATCTAACTAGAACAAATCTTTCATTATTTTTATTTTCGCTGTATAAAGCAATATCAATTGAAAAATTTTTGAAATCAGTAAATTTTAAATTGAAACTTTTTGAATTTTCAACAATTTTAGTACTAAAACCTAATGGACAAATATGTTTTTGTAGCTTTTCAAGTATATATTTGTTGAAATCCGCACTTTCTAAATTAATAGTTTTTTTAGGTTTTAAAATAATGTTGAAGTCATATGCAAGTGTGTCATCAACAATAGCTAACATTTTTTGACCAAATGGAAAGGTTTTTTCAACATAAAAACTAATTTCGCTTTTTTGATTGCACATTTCTTCACAAATATCGTCTAATTTATGTGCAACTCTCGTAATTAAATCTTTATTAATATTGTTTGGGAATTTTACTTTAATTAAATTTAAATCTGAAAAATAAACTGCTTTAGCCATAATCTTCCTCTTTTCTAATTTCTTTCATAATATCAATTATAAATGAAGAAAAAGTAAATTATTTAATTTTGCAAAAATATCTATAAAATAACCATATTAGTAAAAATTTAGTTCTTAAATAAACCTTAAAAAATGTTAATTTCAAAAGTGAGACCTCTAGGTTTTAGAGATCTCACTTTTTAGATTGTCCTTTATAATATAAATTACTTAAAAAATAAAGAAAGGACACTTAAATTATAATTTACGGTGCAAATAAAAATGGTTATTGAAAGGCATTAATAAAATAATAGTTTAAAATATATTATTAATAAAGGAAGAAAATGAGTTATTTACAAAAGTTATTAGATAAATATTGCCCAGATGGATATGAGTGAGTTAAATTAGAGGATGCAGTTGAAATTTTTGATAACAAAAGAATTCCTATAGCTCAAAATAAAAGAATAAAAGGAAAATTTCCTTATTATGGTGCAAATGGTATTCAAGATTATGTAAATGATTTCATTTTTGACGGTGAATATATTTTAATAGGCGAAGATGGTAGTGTTATTGATGGGTTGAATCACCCAATTTTAAATTATGCAACAGGCAAATTTTGAGTTAATAATCATTCGCATGTTATAAAAGCAAAAGAAGAATTTTTAAATAGATTTATCTACCATTTTTTATCTATTTTAGACATTTCTGATATTGTAAGAGGCACACCACCAAAAATGACAAAAGGTAATTTATTAACTATTTTAATACCTAAAATTCCCTTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATACTAGAAGCGGAGCTGAAAGCGGAGCTGAAAGCGGAGTTGGAAGTGAGGGGGAAGCAATTTGATTTTTGAATAAACAAATTGTTAAATTTTACCAACTTTGATAAAAATAATTCAAAAGAATTACAATCTATAGGTTGTTTTATTAGTGGTTTAAGATCAAAAAATAAAGATAGTTTTGTCAATGGAAACCAAAGATACGTTTCTTATTTGGATGTTTTCAATAACAAAGAAATTAATTATTTACCTAACAATTTTGTAAAAATATTTGATGATGAAAATCAAAACGATCTTAATTATGGCGATGTTATTTTTTGCGGTTCATCAGAAAATTTTGATGAAACAGGTTATGCATCTGTTTACACTATAAAAAATGATGAAAAAGTTTATTTAAATAGTTTTTCATTTATTTTTAGATTTAAAGATAACAATTTATTTTTGCCTAAATTTTCTAAATATTTTTTTAATTGTAAAGACTTTAGAGACTTACTTCTCAAATGTATAAATGGTGTAACTAGATTTAATTTATCAAAAGAAAAGATGTCAAAAATAAAAATACCTATTCCTCCTATTGAAACCCAAAACAAAATAGTTTCAATACTTGATAAACTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAAAATAAAATTTATTTGAAAATAAATGTTTTTTATATAATTATTTAGAATAAAAGGACTTATTATGAGAGAATTGATAACTTTAAAAAAGAGTAGAAAAACGCTAACCAATAGGTTAGCTTTTGCTTTCTTTCTTTCTCTCTCTCTAATCTATTAAAAAATATTATTTTTAATTAGTATCCAGCACACACTGGATGTTTTTTAGTTACCTTTTATTCAATTAATTTGTATATAAAAGGAGAAAAATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAATTTAACCTCACTTGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAAGTTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGAGCTGCATTTAAAGGATGTACATCATTAACAGAAATTAATCTTCCTGAAAATGTAAAATTCATTGGTAAACATGCATTTTTAAATTGTTCAAATCTTGCAAAAGTTGAAATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAACAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGAGATAATAAAGATATTAAATCTAAAGCTTTCTATGGTTGTGATGTGTTAACTACTGTAACATTTGCTTCAAAAGTTAAAAATATTTTAAAAGAAGCGTTCTTTGCTTGTCCTCTTTTAAAAGAAGTTTCTATACATAAAGGAACAGACTGAAAAACAGGACTTATGAACAAAAACAAAGCTTTTGATGAAACTATTACAACTGTTAAAGTAATTAATTAATAATTTTAGGACTTGCAAAATTTGCAAGTTCTTTTTTATGCTTATGTTTTCATAATTTTTAATTATGTGAAAAATTATTGTGCTTAAAAATTAAATTAAATATTAAAATATAAATAATTAAAAATTTAAGGAGAAATATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGAGACTGAGCAAGTAAAAACGACGTAACTATTTTTATTACAGCTGATCATGGTAATGCAGAAATTACTGAAGATGAAAATGGTAAACCAGCTACAAAACATACAAGTAGTCCAGTTATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAAAACAAAAATTACGATTCTTTAAAAACATAAATATCATAAATTTGAAATGCTCTTTTTTTATAATGAGCATTTTTTATTTAAATTTAATTATTAAAGTTTTTTAAAAAAATAAATTAACTTTATAATTGAAATATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGAAATAAAAGTGGTGAAATTAACCAAGATCTCCATAAAATAGAGCTTTTTGGAACAAGCGATGAAATAAAAAAAGCTTATGAAGAACTTAAAAAATTAAAGTTAAAACTCAAAGTTATTTACTTTGGAACACACATCGAAATTACTCCAAAAGGTGTTGATAAAGGCTATGGTTTAAAATGAATTTGTGAAAACATTTTTAATTGCAATTTTGATTATGTTATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAGAAATAGGCTATATTCAGCTTATTTTTTTAATATTAATATTAGATTGTAAAAAAATAAATTAAATAAGTAATCAAAATTGAGTTTTAATATATAATTTTGTTAAGCAAATTATATAATTTGTCTTATTTTAATTAAAATAAAAATTTAAGAAAGGTAATAGGTTACTTATGAATTATTACAACGATTTTGATATGAATCAACAACCTAAAGCGAAATTCTGAAGACCTAACGAATGAAATGCATCATTGGTGCAACCTAAGAAATGATGATACTTTGGCGTTATTGGACCAATTATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGATTAGGCAAAAGTCAACAAGCATTTAAAGCTGCTAAAGTTTTCTTTATAATGACACTTATATTGTTCCCATTCTTGCTTATTTGATGTTCAGTTTTAAACTCAAGAATGGATCCACCAGAACCTGAAGAAGTATTTAATCCTTATTCAGCTGCTAATGGTTATGGATTTAATCCAGCATGAGCCTCACAAACTCGTACATTTGCCTTAACAGGCCAATATACAGGTTCTTATGCTGCTCAACCACAACCTCAACAAACACAACAATTTGGTCCACAACCAAATCCACAAGCAACTGGTATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGAATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAAAAATTTAAACAATATTAAAAAAACTTGCAGACGTGCAGGTTTTTTTAATTTGCCTATAAAATAGAGCTTTTAGAAAAACAAGCTTAATTAATTTTCACTATATAAAAAGTTAATAAACATTATTAATATTGATTTTTAAATATTAGTATAAAATTAAAGTATTATGGGTTTTAAATTTAAATCAACAGAAATGCGCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGCTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGAAAAGCTGTTACAGAAAAAATTAAAGAGTTATATGAAATAGGACAACCTGTTTTAGTTGGTACTGCTCAAATTGAAGATAGTGAAATCTTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGAAGAGAACAAGCTATTCGAAATAGCAATGATGATACTGATTATATCAATTGATATGAAAAAGATTTAGTGCTTGAAATTGTTGATATTTATTGACAAAACCATATTGACACAATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAATGTATAATAATCATGAGCCGACATTGAGGTTAAAACTTTAATGGTGAAGTCCTAAAAAGGACTTTTTTTTATTGTTATTTAGACCAAAAATGCCAAAAATTGGCTAAAAATAAGCTTTTTATAACTTTTTTCAAGAATAGATAATGTTATAAAGTATAATAAAAATATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGACAAGTTCTTTTAAGTGTTTTAATTGGCTTAATTTTTGGTTTAGGACAATTCCTTTTAGTCCAAATAACAGGACTTTATAACTTAGGTTTAGATGCTTTCTGTCAATCAGTTGCTCGGATTGCAAAACACTTTATTACTGATGATAACTGAAAAAATAATATATATAACCTTTTATTCTGAACAGTTAACTTATTAGCTAATATTCCTTTATTTATATTAGGTTATAAAAAAGTTAATAAGCGTTTTGGGTTTTTAACAATAATTTTTATGACTACTGCTACTATTTTTGGGTTGATAATTGGTTTCATACCAGGTAGTGAAAATTGATTTATTTTTACAAAAATTAAAGCAGAAGAATTAACATGAGATGAGGCAAAAGGCGGCAATTTAACAATCTTTGTTTATGCATTAGTTTGAGCATTTTTACAAGCAGGAATAGCAGCGGCTTTATTAATAATTAACTCAAGTACAGGCGGAAGTGATGTCTTAGGAGTTTATTGATCACACAAAAAATTTAAAGATGTTGGTGCAAGTTTCATTTTTGTTCACTTAGCATTTTTAACAGTTGCTAATTTAATAGGAACTTATATTCCTTTAATTACGCCAAAAGGATCTTTTAGAGAAGGCGCTTATAAACCAGAATATTTCTTTAATCCTTCATTTATTGCTGGTATTGTTATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAGTTGTCTTTTATTTTTTAAATTTTAGTATTCCAATTATTTTTTTAAATTATAATTTTTTATTATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGAACAAGTTTAGTATGAAAAGATAGTGACAAACTGATTGAGTCCTTCTGAGTTTTAGTGATTTATAATGTGTTATTCTGGCTGTTAATATTAGTAGCTAATATTCCTTTATTTGTTTTAGCATATAAAAAAATTAGCAAAAAATTTGGTTTCTTATCTATTATTTTCTTAGTTGTAGCTTGTATTACAGGTTTTTGTATTGGTTTAATTCCAACCTCTGAGCAATGATTTATGATTTCAAAAATTGATTCAGCTAATTTAACTTGAGAAACAGGTAAATTTAAAGATTTTTCAATTTTCTTATATGCAATACTATGAGCATTTTTACAAGGAGTATTTGCTTCAGCTTTATTAATTATTGGTTGTTGTACAGGAGGAGCTGATATTCTAGGAGTTTATTGATCACACAAAAAATTCAAGGATATTGGTGCAAGTTTTATACTTGTTCACTTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAAAAATCGGGAACCCCGATTTTTATTATTAAATTTTATTATTTTTTGCCTTTGTGGCTAATAGCATCTCTAACACGGCCTGTTGTTTTGTGAATCAATACAGAACCACCACGTTTTTTAGCAATTTCATTTGCTAATTCAACAGCTTCTTTTTGAGTTTTAACAATTTTTGTAGGTCTTGAGTTCCCAACACCTTTAACTTGTCAACCTAAGCCTTTAGGAGTTACGTGGTATACTGTTGCTTCTTTGTTTTTTGTTTCTGCCATAATTTTCCTTTCATTGTTTTTATTAAGTGTATTAATATTATAAAGAAAAAAACAACAAATTTAAAATATTTTTTTAAAGAGCATTTTTTCCAATAAGCCTATTTTAAAAAGCTATAAGTCACAATTTATTTTTGAACATAAAACCTAATTTTTTAAACTAGTCTTCAATTTTAGCTAAGAAGTCTTTAGATTTTACATTATTTTCTTTAATTGAAAATATAATGTTGACTTTGCCATTTTTATCAAGAACTGTATATAAAAATCCATGAGAACCTGCATTTTTACCTGTAATTTGTTTTGAAGGATGTGTTATAACTTGAGATGGTTTTTTCAATCCAGTTTTTTTGTAAGCTTCAATTGCTTCTTTTGTTAATTTAAGCGAAGTTAGATTAATTGGTTTTTCCTTTTTATTATTACCTAATTTTTTTAAAGTAAAAGTATAATTTTTTGGATTAATATCAATTCTCATGTTTTTGTTGTTCATGTATTCTTTAATTTTAGCTAATTGAGTAGCATATCCAGGATCAGTTTTGTCATAGGTGAATATTTTGCCTTCTTCTGCCAGCTTTCTTAAATCTTCATAAGGATTTGCTGGAACTTTACCTTGTAATTCAACATGAGTAAAGTATTCTTTATCAATGTTTGTATTTTTGTAAGTTACTTTAACTCTTAATTTTCCTGCTTTTTGTAAAGCAAGTTTTTCGGCATTATTTTTTGGTCAATCAACAGTTACGCCAGTTAATGTGAAAATGTCATTTGTTGTTGTAGGTACATTTAATTTAGTTTTTTCATAGATGATGTCATTATCTGTAGCCTTTGTAACATCACAAGGGTTAGGTAATGAAAGATTAATTTCAGCTAAAAATGTATCTTTTAATTCTACAAATGATTTTTCTGCAAAAGTTAATTCAAATTTCTTTTCTTTTGGAGTTGATTTAGTTGTGTATGAAACCGTAATAGTTAATTTACGTGTTGCATCATCAAAATTTAATGTCATTAAATTATTTAAATTAATTGTTGTGGCTTGAATATTCAATTTTTCAAGATTTTCTTTATTGAATTCTTTTTCTTCATTTGTATCTGGATCAGTGTAGTAAAGTTTTACTAAATTTTGTTCTTCTTTAATTTCTGAATAATAGTTCTTTTCACTAATTTTAGTTTTTAAAGTACCGATTAAGTTTTCAAATTTGTCTTCTGAATATGGAGCTGGTTTTTTGAAATCATTAATTTCATATTCAACTTCAACATTTTCTTGACTTTTATCTTCAAAACTTAAAGTTAATTTCAATTTTGCTTGACCTAAATTATTATTAGCAACAATTAATTCTGTTTTATTAATTGTAATCTTTTCTTCACTTAGATATTCGCTTAAGGTTTTACCACTTGGAGTTGTGGCTGTAACTTGAGCCAATAAATCACTTGTGTGAGCTGCAATATATTCTTCAATTGTTTTACTTGCTTTTTCATTTAAGTCAACATTTGTTAATTTAGCCATTTTTTGTTGAATGGTTTCTTTTGTAACAAAACCAGTTATTTCTTTGTTGTATGCATATTCTGGTTGATTTCATTGTTGTCATTTTGCAACTAATTTAACTTTTCCTTCAAAATTGTCAACAACTTCTAATTGATATTTAATGTAATTTAAACCTTCCATGTTTTTAAATACAGATAAATCATTTTGATAATCTGCATTTGTGAATGTAATTTTTTCTTCTTTTTCTTGATCAGTTAGTGCATTAAATTCTTCAACATATTCTTGTGCAGTTTTAGTTGATTTATCTTTTAAATCCACATTAAATGCTGCTTCTAATTCACTATTAACTTTAATTTCTTTAAAGCCTGCAACTACTTGACTTCAAACATATGTTTCAGTTTTAGTGGCGTCTTTTCAACCAAAAGTAATATTAATTGATAATCATCCTGAATAAGGTTCAGTTTCACCTGATGAGGTATCAGCTTCTTTATTTTTAATTTCAATACCTTTTTCATTAAATACTTTTGTTACATCAACTTGTGTTTTATTTTTGAAGTATCTTAGATCTGTAATTGTATAGTTGAAAACATTGAAATCTTTTTTGTTTTCTAAAACATCAATAGTATCAAAAATACCTTCTAATGTTGCTTGTAACGATTCAGTTTCTTTTTTGAATCCAGTTAATTTGATTGTAATTTCTTTTGATTTAGTTTTTGCTTTATTTTCAAAAGTTGCTTTAATATTTGCTTCACCTTTTGAAGCACTTACTTCTTCTATTTCTATGCTTTTAATTGATATTTCATATTCTTTTAAGAAAGTAGCTAAATCTTTGCTATTTGAAGTTGCTATTGACAATTTAGCTTGTAATTTAGCTAGATCATTAAATTGATTTTTGTATTCTTTAACTGTGTATTTGTCTTTTTCTGCTAAGTCCAAACCAGTTATAAATTTGATGTTTTCGTCAAATTTGTCAGTATCTACATAAAATCCTACAACCTTAATTGTGAATTTTTTAATTTCTTTATTTCCTTCTTTTTGAGTAAGGAAAATTTCTAATTCACCCTTAGCTTTATCTGCTTTTAAGTTGTCAATTATATATGTATATTTCTTTGTTGTACTTTCAAATTTAATATCTTCTTTTTTAATTTGATCAGCTTCTTTTGTAGTTGTGCTAATCTTATATACTTTGTTTGTAGAGTCATAATCAGCATTCTTAATATCTGCAATAGACAAAAGTTTAAAGTCTTCATCTGCTTTAATTTCTTCTTTTGTTGTGTTTTGACATTGAGATGAAATTAAAGGTAAAACACCTGCTGTTACGCTTGAAAAAATAAGACTTAATTTAATTTTCTTCTTCATATATTTCTCCTTCATAAGAATAAATTCTTATGCTAATAAATTATCTTAAATTCCTATTATTTTTTAAATAAATGGAAAAAATATCCACATTTTTCAAAATTAATGAAATTAATAAATTTCTCATTTTTTAAAGGAATTTACCAAAAACACTATAAAACAGCATTATTGGATAAAAGCGGTATAAATTGTGAAAATTTTATGTGGAATAACTATGTTAAAATTACTTTTTTTGTTACCAAAAATAATATAATTCTATAGCAATAAATTTTATAAGAGAGGTGCAAATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAAGCAATTCAAGAAAGAGTTGTTAAATTAACTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGAGTTATTAGATACAAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGTAGGTGGAGGTACATCAACAAGCGAAACAAGAAAAATAGAAGTCAAAGCTAAAGCAAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAATCAAGTCAAGAAAGAGTTACTAATTTAATTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGACTTATTAGATATCAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGGAGGCGGAGGAACAACAACAGGTGACTATGTTTATGATAAAAGTAATAATTATTATTCTTCTTTAGAAGGTTTAAGAGGAGTTGATTTATTTAATGCTTTATTAAATTTACAAAAACAAAAAAGAAGCAAACTTACAAATTATGCAGATTTAGTTAATAGAATTTATAGAAATGCATTTATAGATAAATATTTCGAAAAAGACAACACTATTCTTGATGTATATTCAGAAAATCCAAATGGAAAAGACCCTTATGTTTTTAAAGTTGATGAATATGATGGAAGAGGCGGAAAATCAACAGGAAAATCATATGGTAATGGAGAAGGATCTATGTTTAATCGAGAACATATGATTCCTCAGTCATGATTTAATAAAGATGAGGCAACAAGATGTGATGCTCATTTTGTTTGACCTACAGATAAAAATGTAAATGCTTGAAGAGGTAATGATCCTCATGATAATATTAGTAGTCCATCTTGAACTTCTTTAAATGGAACTAAAGGGAAAGATGGTGTTGGTTGTGAACCTATTGATTACTTTAAAGGAGATATAGCAAGAGCATATTTCTATTTCAATGTTACACACCATAATGGAACACATAATAAAGGTGAAAGAGTATATACTTCAACTTTTCCTTTCTTTGTAAAACACTATTTAGATGTATATTCTAAATGAGCAACAAATGATCAAGTTGACTTAATCGAAATTGACAGAAACAATAAATTAGATGAAATCTACGGTGGTTTAAGAAATCCATTTATTGATTATCCTGATTTAATTGAATTAATTTGAGGCAATGATACAACTAAAAAATTTGAAAATCGTGGTGTAATCGTTAGTGTAAAAACACCTTAAAATAATATAAATTCAACAAGCAAGAAATAATTTGCTTGTTTTATTTTTATCTAAAAAGCCTATAAAATAGTTGATTAGAGTAATAACACAAAAAATTTATTTTAATTAATTAAAATACTAAATTAAATCTTGCTATTTGAGTTTATAATTAAAATATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAAAAATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGAGCGGTAAATTTTATAAAAGACTTAAAATTAGAATTAAATAAAGTTAATGATATTGAAGGCTTTTTAGATTTAGTTTTAACTCAAAAAAATCAAAAACTAATTAAACAAAAATTAAGTCAAATATTCAAATTTGTTAACTTAGATGGCAATGTAAAAGACATTATTATAATTTACGATTTAATCTGTTTTATAGGTTTATTAGTAAATTTAACAAACACAGAATTTGAACTTTTACCATATGATGCATGTAATATTATTTCGTAAATAAGGAGGTTTTTTATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGAGCAACGCTTTTAGAAAAACTTAAAAAAGAAGGATTCTTAAGAGTTAAAGTTGATGGTGAAATAAAAAACCTTGAAGATGAAATTAAATTAGAAAAAAATATTAGACATACTATTGATATAGTAATTGACCGTGTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGAAGAAGTATATTTGAAGGTGGAATTAAATATTTTGAAAACACAGTCAATACTCAAAATTTAGAGTGACAAGAATTTGAAGTTTTATTAGATCATTACAAAATTGATAAAAATTGTCCAATTGAAGATTTAGGTAAAAAAGACTTAGAAATCATTAAATATGGATCAAAAGAAGAACTCGACTTTGTTGTTGTTTCTGAAAGTGGAAATAAATGAAGAAAACACAAAGTTATCGAAGGTATTTTGACTAAAGTCCAAAGACTTTATATAGAAACAACTAGCGAAAGAATTCGTGACTGACTTAAAAAATACATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATTAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAAATCACAATTTTGAAGAAGCACAAGCTGCTTTTTTTATTTTTCTAATTTGCTAAATTTTGACCAATTAACCTATAAAAAAGCCTTAAAATTCAAAAAATAAATTTTAATTTTTCTTAATTTAAGAAGGCTTGAAAAATTTTGAAAAAATAATACATTTTTTTTCTTGGTATTTTAGATTTTATTGGTAAAATATAAACACATTTGCTTTTTTATTTAATATTAAATATTTATTATGCAAAAAACCTTTTTATGGTATAAGCTGACAAAAAAGCAAATTTTATTATGAGAAAAAAGGAGTATTACTGTATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGAAACGTTCCTGACTTTTTAGTTACAAGTAAAGAATCATTCGAATGATTCAAAAAAACTGGTATTGAAGAATCTTTAAGAGAACACTACCCAATTACAGCGACAAACAAAAAAGTTTCATTAGAATATATTCCAAATTCTGCAGATTTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTGGAAGTTTTATCATCAATGGTAGCGAAAAAGTTATCGTTAGTCAATTAATTAGATCACCTGGAGCTTACTTTGGACGTGGCGTTAGAAATAAACAATCTGATGACCTTTTCAACAAGGTAGAAATTTTACCTCGTATTGGTTCTTGGGTTGAAATCTCTCACAAGGTTACAACTAAAACACCAGATAGCGTAAAAATTAAAATCGATAAAAACAAAAATGTTAACTTAGTAACTTTCTTAGGTTCACTTGGTTTAGATCGCCAAATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGAATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGATCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGATTAATTGATCCTATTACAGGTAACAAATTAGATAAACCAGTTTCAGTTGGTGTTATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGAGCTATTGAATCTTATGGTGCAACAAATGTTCTTCAAGAAATCTTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGATCAAATGGTGAAGTAACTAAACCAGAAACAATTAACTATAAAAGTTACAAACCAGAACGAGATGGTCTTTTTGATGAATTAATTTTTGGACCAACAACTGACCACAAATGTCCTGTTTGTGGTACTAAATATAAAAGAAGTGATGAAGGAACTCAATGTTCAAAAACTCCTTTATGTGAAAAATCAAAGGCTACAATTTTAGCTAAAATTTCACGTCGTAGCCGTATGGGTCACATTCATTTACAAAATCCTGTTGTTCACTTTTGATTCTTCAAAATTGATCACTCAATTATTTCTAAATTACTTGGTTTAAGAGTTGCAAAAAGTTCTAAAACAGTTTCAAAAACAGATTTAGAAAAACTTATTTACTACAAAAGCCACATTGTTTTAGAAAGTGGTGGCATTAAAGCTCTTCAAAAGAATGCAATTATTGATATTGCTGAAGCTGCAGTTATTTATCGTGATGCTTTACTTGAAATTAGAAGCAAATATTCTGAAGAATCAGAAGAATATGAAATTATTAATGAATCACTAGAAGAATTAGAATCATATGCAACTAGTAAAATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGTCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGAGAAGAAACCGATGCTCCTATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGACGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGAATTATTAAAGAATTAATTAAAGGTGACAACCAAATTAACTCAATTAAAGCTGGTAAAAAAGCTGTTGAAAATTTAGATCCTATTATTTGACCTTATGTTGAAAAAGCAATTCAAGGTCGTCCAGTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGTAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAATGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGCGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGACATACAGAAAACTTAGTACGTATAATAAAAGAAGAATACGAAAAAATTAATGAAGAATTAATGATTGAATATCGTAAAGTTGATCAAGAAATTGGCTGAAGAGTTAGTGATTATACAAAAATGCTTGAAAGAGTATGATTTAGATATACAAACTTTGTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGAGCCGAAATTAAAGACTCAATTGAAAAAGACTTAAAAGAAGTTACAAAAACTGATCCAGATAACCCATTATTTATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCACGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTGTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGAGGTAAACCAGCTGTTATTGCAGAACACTATGGCAAGATTACAAAAATAGAAGATAAAAAAGATACTTTAGGTAATGCTAGTGATTATCAAGCTATTACTCTTGAATATGAAGATGAAGAAGGCAATACACAAAGCAAAATTTATGCTGCTCGTAAAGATCGTCGTCTTAGAGTTAAAAAAGGTGATAAAGTTATCCCTGGACAAAAAATTGTTGAAGGACCTATTATCCTTAATGAATTACTTAAAGTTGCTGACACTCGTTCAGTTCAAAACTACTTATTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAATAAATAAAAAATAAATAGCTTTTACCAAAAATATTGTTTTATAGTACTATTAGTAAAATTTAGCTATTTATTTTTATTTTTTTGTAAAAAATACTCTTTTTGACTAATCAAGTTATTTTATAGTATTTAAAAAAACAAATTAAATTTGGTATATAATTAAAGTCCGGTAATATTTAAAATATTGCCGGGTGAATTTAGGCGCCCTTCACTCAGGCGCAAAAAATTATTGTTATCATTTATTTCATTGAAAAATATTTTTATCGATTTTTATAACTTTTATTAAATTACAATTAATTACATTTTCACATATCATTTATTTATATTTGGTAGTTTATTAAATCATAATGTTTAAAAAACTCTTTTTAAAGTGTTGTTAGGGAATTTTAAATTCATATCAATATGCTCCTTTCTGTGGATTTCTTTTTGTTTTAAAATAGAAATTTTGCATAATAAGAAAATCTTTAAAATTTCTTATATATATTGTTTTTTGTTAATATTATTATCGGGTATACATTTTTTATCCTCCTTTTAATATTATATTTATATTATGAATAATACGCGAAACAGGCGTGGCCTGTTTCGCTTTTATTATTTTTTTAGTATAATAAGGTATATTTATTACTTATAAATAAATTTAAGTATCTTAAATTTAATTAGGAGTCATTTATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGAAATTTACTTTTAAATTATGATCCAAATAAACCTTTAGACCCAAAAGATTTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGCGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGAAAACAAAATTTGAAGTTATTAACTCCAAATATTTTTGATATACACCCTTCAAGTTTTGGCTACAAAAAAATGGCCATGGAAGTATTTGTTAAATTAATTAATCCATCTCGTGATATTAATAAATACCATGAAAATAAAATTGAATGAGATAATAAATACTTACAAAGCGACCAAGATTCTTTTGAATACCAAATCGAAGTTAAAAATCCATTTGAAAAAATCAAAACTATTTTCAAAAATAATTATGATGGTTACTTAAAAGAAAAAGATAGTTTCTTCTTAGCTTATGAAAATCAATTAAATGAAGGCAATTATATTAATAGAGTTTTAAACAATAATGGTTTAGCTGATTTCTTATTTGTTGATTTAATTAACTCAATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGATTCTTTAATGGTCAATTTATGGCTAAATTTTTATATGATGTTCAAAAACAATTATTTGAACAAGACTGAGATCAAGATAATTTACCAGGAGCTAAAGACTATCAATTTAAATACCTTTTAGAAGCAATTAAAACACAAACTTTAAAAGAAGAAAGAATTATTGAATTTATTACAAGTTTTTGTTCAAGTTCAGTAATTACAACTCAAAAAGATGAATTAAAAACCATTTTAGAAGAAATCTTTTCAAATATTGTTGCATATGAAGTTAAATATGAAGACATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTTGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGACAAGAATCAACATTTAATATTATTAAAATTGTTGCTAAATCTAACCTATACAAAAATCGATCAACAATTTCACAATTAATCAAAAATATCTTCAATTACTTAAATACTAAAAACTCATTTGCTAAGAGAATTGTTGATCTATTTTATGACAAAAATCAAGCAAAAATATCAGAATATATCTCAAAAGAAAATCTTACTAGTTTATTCGAAAAGGTCTTTAAAACAGAAGAATTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGACTTGCTAATAAAGGAAGTGTTGACTATATTGTTCTTAAGGCTTCAAAATTTATTCTTGATGTTTCACAACAAAGTTCAATCAAAGGAATACTAACTTCATTAATTTATGAAGCATTATCTAAAGAAAGAAATTTAGTTAAAGATATTGATCAACAAGAAATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGAGAAAAGAATACAGTTAAATTAATTAAAATTGCAATTACTAAAAAAACAATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGACTTGAAGCTATAAGCAAATTCATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGAAGAAAAGAACACTGAACTAATAGTGAAAATGATGACATTATTATTCAAAAATTCATTCGTTCATTTGGTATTGGATTACTTAAAACAAATATTTTAGATGATATTGTAGATAGCATTTTTGAAAGATTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGAAGATTTTATAGCTTTTTAGCTTCAGTATTGTATTCAAACACACCAAGTGGATTGTTTTGAAATGGAACAAGTTTGACAGCTGAAGCTTACTTAATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGAACTGGAACTCTTCATAGTCGCTCAAACAGTTTAAATGACTCTAGATATGGTAGTGATCATGTTCTAACTTACATTTATTACAAAGACAAAAAAGATTCTAAATTTACAAACAAAAAATTCAAGCAAGCATTAATTGAATATATGCATCAAGGTTACATGCCTTATAAATAAAAATAGAAAGAAGATATTATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGAGCAAATGTCGCAATAGGCACTAAAGCAACTTTAATTACTAGTGACAATAAAAGATATAGCGGACTTTTCGGACACACTTCAGTTCACATTATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAATATTTGTGTTAAATAACTTTTACTTTATAATATTAAATAAGTTATCACAAAAACGAGGAGGTTAATATGGGTACAGGTTCATGAATCGGATTAGTTATTGGTATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAATAATTGTAATAATTTGCAAGCAAAATAAAATATTTGCCCTAGTTAGTGAGCAAATATTTTATTTTTTCTTTTTAAAGAATCATCTTCTTTTTGGTCTTTTGTTTTCTTCTCCTTCAACACTACGAGCATAGTGACATTTAGGGAAGTTAGCACAACCAACAAATTTTGTATCATTTCTTTTATTATTACGAACAATTAATTCACCGCCATCATCTGGACATTTTTCATTTAAATATTCAAGTGCAATAGCAATTAATTCTAATGTTTCAGTTGCATTTTCATAAGTCTTATTAAAACGTTTTCAAAAATCATACATAATAGGTTGAACAGTAGTTTTATTTGAAGCAATTAAGTCTAATTGTTCTTCAACTTCAGCTGTATAGCCTTCATTAATAATATCTGGAAAACCATCTAATAATTTACTTAAAACTAAACGGCCAAATTCTGTAGGATGTAAACTTTTATCAATATTTTCAAAATATTTTCTTTCCAAGTTTACTGCAACAGTAGTTGCAAAAGTTGAAGGACGTCCAACTTTAATATTATCTAAAGCTTCAATTAATGAACCTTCAGTGTAACGAGCTGGTGGTTTAGTTTCATGATTTGTTATTTGATATTCTAAAACATCCACTTTTTGTTGTATTTGATAATTAGGATCTTCAATATCTTCATTTTCATTTTTAACAAGATAGTAACCTGGGAATATGGTTTTACTTACATTAGCTTTAAAAGTTAACTTATTTTTTTTAAAGGTATAAGTTTTATTAGCTTTAATTGGTGGATTAATTAAAGACATAAGTGTGTGTTCATAAATTAGCTTATAAACAATGTATTCACTATTATCTAAATTAAATTTAGCTTTAGCAACTTCAGGCAGTAAGTTTATATCAGTTGGTCTAATAGCTTCATGGGCATCTTGATCGCCTGCAAAGCCTTTGATTTCTTGAAGAATATATTCTTCACCGAATTTATTTTTAATATAACTTCTAGCAATATTTAAGAAATGTTGACTCAATCTAGTACTATCAGTTCTAGGATAACTAATCAAACCACCATCACCATAACCTTCATAAAGTTTTTGAGCTGCAATTTGAGTTTGTTTAGAAGTTAGCGGACTTTTCTTATATAAAACTGCTTGTTTAAGTGGCGTATATTTAGCTAAAGTTTTTCTTGCTTCTTTAATATCTTCTACTTCTAAAACATTAATTGGTTCAATGCTAATTTGTTCTTTAACTTTTTCTAATTCTTCAGGATAAACTCAGTTTTTTTCTGCTGCTGGATGTTTATCTATATAAATAGAAGCACTTAATGTTTCATTTAATTTAGCTTCAACTTTGTGATAACCACGAGGCACAAATGCATTAATTTCATCTTCACGATCAACTACTAATTTAAGAGCAATTGATTGTACACGTCCTGCACTTGGAGTACGAGGACTATTTGAAATTTTCTTTTGCATTAATTGGCTTAATTTGAAACCAATAATTCTATCAAGCATTCTTCTTGCTTTTTGTGAATTAATTAAATTTTGATCTAGTTCTGTTGGATTTTGAATTGCTTTTTGAATAGCTTCTTTTGTTATTTCATTGTATTTAATACGGTAGTATTTTTTCTTATTTTTATTACGATCTTTTTCAATTAAAAAGTTAACTATGTGATCTCCAATTGCTTCTCCTTCTCTATCAGGGTCAGTTGCAATATAAATAACATCGGCTTTTGCTATGGCTTCTTTAAGGTTTTTAATAACATTTTTTTTACTATTGTCTAATGAGTAAAAAGGCTCTCAATTTTCTAAATCAATTCCTAAACCTTGTTCGCCTTTCCTAGTACTCATAATAGCGATATGACCAACAGAAGCAATCACATCATAATCGCTTCCTAAATATTTTTTAATTGTCTCAACTTTGTTTGGAGATTCCACTATAACTAAATTATTCATAACTTTAATTTATCACAATCTTGCAATCTTGTTATCATTTTTTTAGCTATTTTTTTGCCTTATTATATTTAATATTAAAGTATTAATATTAGAGTCAAAAATATTTAAAAATTTATTTACACAAAAATTTCACTATTGAGCATTTAACTTAATTTGCCTATTTTATAGTGCTTTTAGTCAAAACTTCAAATTTACGAATTTATATTTTTAAATATAATTTATTTAACATAATTCTTTTTATTGAAAAATAGTTATTTTTTTACTAGCTAGTAATTAAATTATATATATAATATAACAGAGTATTTTATTAGGATAATAATACTCCTTGGTCACTTAAGACCCTGAAGTCCAAAAGGAGAAAGAATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGAAGACATTTAGCAATTAACTTAGATTCTGAAAGAGGATATGGGAAAGTTAAAAAAGAAAGCCATAGAGCAAAATATGAAGAAAAGAAAGAATTCAAAAAATCAAATAAATCAGCTGAAGCTTGTGAAAAAGTAGCTGAAGAAAAAGCTGCTAAAAAACCTAGATCAGCTAAAAAAGAATCACAAGAAAAAGAAGTTAAACCACGTCAAAGTAAAGCTAAAAAAGTTGAATAGTTAATTAACTTCTCGTAAGGAGAAAGCATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGAAGAAATTCAGCAGAATTTTTAAATAACTATGCTTCAAAAGGAGCTCTTGTTTCAATTGAAGGTTCATTTACTTCATCACAATATGAAGGACAAAATGGAACAGTTTGATCATATGAAGTTACAGTTGACAATATTTCATTACTTGAACCTCGTTCAGTAAGAGAACAAAGAACAGGCAAAAGTGGAGAATATTCAGCAACAACTCCTTCATTTAATCAAGCAAATTATCAACAATCTAAAAATCAAAATACAACTAAAAAAGCAGCATCAAGCCAAACTAAAAATAATTCAAAACAAAATGAAAACCAATTTGGTGGATTTTTACTTGATGGCGAAGATGAAAATGAAGATAGCTATCATACATTTTCAATTGATGTTGATGAAAATTAGTAAGGAGAATCATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAATTTAATTATTTATAAATATTAATTTAATCACCTAACTAGGTGATTTTATTTTACTTATTCACTTAAAAAATAAGTTATATAATTGAAACACTATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGAACTAAAGAAGCAAATGATTTAGCCTTAAAATATTGACCTGGAGCTTATTCTATTATTACTAATAACCAAGGTTTCAGAATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGATTGCGATAAAAAAACTGCCTAAGGCAGTTTAAATTAAATAGTTAATTCTAAATCTTTTGAAACAGCTTCATAACTTTCTTTATCATTAAGATAAAGCAAGTTAAGAGCTGCTTTTTGTTTAGCTAAAATAACATCAGATTGAGCTTTTGTTAATTCATTTGAGCCAGTTGAAATTAAAGTTTTTTCTTTAATTGATTTGTAAAATTCTTCTGCTTCTTTTTCTGTAATTTTGTTTTTAATTAACAATGAAAAAGTAAATTCAATAATTGCGTCTAAAAGAGAATTACGAACATTTGTTTTTGCTAAATCAAAAATTTCATAACCATCTTTTTGTTTATTTTCTATTGCATTTCTTAATAGATTAGCTTTATCTGGAGGTAACATTGCTAATTTACTTTTAATAAAATCATTTACTTGTGTATCTAATAAAATAGGTGGTAAATCAAAACCATATTTATTTCCAACTGTTTCAATTGCTTTTTTCATGTAATCTAATAAATAACTTGAAGCCATTTCATTTTTCAAATTCATTGACAATTGATTATAAAGATCATCAAGAGTATTAATGCCTTCAATTTTGATTTCTTTTATGTTGTCATTTGTTAATTTAGTTTTAATTGGACGAAAAACTTTAGTAACTGTAATTTTAATGTTTTCATTTTCTGGTGTATTTACTGAAAATGATTCATTAACTTTACGATTCATTAATTGACTATTAATTGAGTTAGGATTTTCATCTTTTACAATTTTTTTATCTAAATCTTTACGTGACAATATTTCTTTTTTGTCTTTAGAAACTGAAATATTATATCTAACATAATCATTTTCTTTTGATTGGTCTTCAACTTCTTTAAGTAAAGGATAATCATCAACAAATTTTTTGTAAATTTCATCAATAGCACCTTTTGTCACCATTTCATATTTACCATCTGGTTTAATATTTATGTCAATTTTATCGAATTCAGCTTTTGGATAAATTACAATTGTAACAACAGCTTTAGCTTTATCTTTAGTAATATCTTCGACAACAACAATAGGCGAAAAAGCTAAACAATTTGGATATTTTTCTTTTAATTCTTTACCCATTGATTCAACTATTTTATTTGTATCTGATTTAAAAGCTTCTTCAATTAATTGGTCAGGTGAAATTTTCTTGCCTTCTTTAATGCTTTCCATTAAAAAATTATTTTGAATTTGTTTTCATTCTTGGTCTTTAAATTCAATGGTTTTGGTTTCTAAAACATATTTAATCATTTTTCCTCTTTTCTTTATTTATTATATTTAATAGTTCATGATTTTTTCATATTCATCTAGTGTTCCATGATAAATAAAACTTTTATCAGGAGCAATTTCAACAATAACATTAGCTACTTGGTTAACTAAAGCTCTATTATATGTTGTTACAATAACACCACCCTTATAAGCTTTTAATCCTGCAATGACACTGTCAATGCTTTCAGCATCAAGGTGATCTAAAGGTTGATCTAGAATCAAGAAGTTAGATTCTAAAAGCATCATTCTTGAAAACATCAATCTCGCTTTTTCTCCACCTGAAGTTACTTTAACATTTTTAAATACTGAATCATTTGAAAATAACATTCTTCCTAAGAAGCCTCTCATTCTTTGATCACTAACATCTTTGGTTTCTTCAGTTGAGTTGTATGGAGGTCATTTAGCAATTCATTCTAGAATTGTTATATCTTCTTTAAAATATTCTTTATTTTCATTTGGATAATAAGTGTGTTTTATTGTTTGGCCCCATTTTATAGTGCCTTTTGTTGGTTTTATCTTACCAATTAAGCATTCTAAAAATCTAGTTTTAGCAATATCATCTTCACCAATTAAAACCATTTTTTCACCACGAGCTAAAGTGAATGAAACATTTTGGAATAGTGTATTTCCTTTTTCATCAACATAAGTCAAACCTTCAACAGTTAGAATGTCTTTACCAGGTTCACGGTTAATGTCTCAATTAATATAAGGATATTTACGATTAGAAGGTTTAATTTCATCAAGAGTAATTTTTTCTAAAGATTTTTTTCTACTGGTTGCTTGTTTACTTTTAGAAGCGTTGGCGCTAAAACGAGCAATAAATGCTTTAAGTTTTTCAATTTGTACTTCTTTTTTAGCATTTGATTGTTTCATTAATTCTCTAGCTAACTCACTACTTTCTTTTCAAAAAGTGTAATTACCTGTATAAATTTTTGCTTCATTGTAATCAATGTCAACTATGTGAGTGCATATGTTATCTAAAAAGTCACTGTCGTGGCTAACTACTATAACAACATTTTGATAATCTGCTAAAAAGTTCTCTAATCATTTAATTGCACGCAAGTCAAGATTGTTAGTAGGTTCGTCCATGATTAAAATATCAGGATTACCAAATAATGCTTTGGCTAATAAAACTTTAATTTTTTGATTAGCAGTTAATTCTGACATTTTTGATTCTCATTTATTTTTTGGAATTTGTAAACCTGACAATAATTCTTGAGCATCATTTTCAGCAGTTCAACCACCTAATTCGCCGAATTTTTCTTCTAATTCTCCTGCCCTTGTATAGTCTTCCATTGTTGCTTCAGGATTTGCATAAATAGCATCTTTTTCTTGTTTGATTTTATATAAATCAGTATTTCCCATAACTACAACATCGGTTACATTCATATTGTCATAAGCATTATGATCTTGGCTTAAAACACTAAGTCTTCGGCTTTTTTCAATTATTATTTGGCCGCCGGTAGCTTCTATTTCACCAGCTAAAATTTTTAAAAATGTACTTTTGCCAGCTCCATTGGCTCCAATTATTCCATATGTATTTCCAGCTGTAAATTTTAAATTAACATTTTCAAATAATTTTTTGTCGCTAAAAATTTTACTTAGATTATTTACTTCTATCATTTTTCCTCCTAAAGCATAATAATTATTTCAATTATAAATTAAAATAAATTTTAAATTTAAAGTTAATAAAAAAAGCTACAAACTATACTGCAATAGCTGCATCTCTAGCAATTACTAAAAATAATTGAGGTGCATTTAGTTGTAAAAGTTTTAAAGCTATTTTTATATTAATTAAATCTTCTTTTACAGTGTTAACATTTTTGTTTTCATTTCTATTGTAATAGTTGTTTGATTGAAATAAAATATTTTCGCTTAGATTTAATTCTTGATTTACTTCTGTGTCATGGACTTTTATTTTTTCTAATGTGTAAACTAATTTTTTAACAATGTCTTCAACAATTTCTCTTCTAGCACTATTAATCGCATTATCTATTATTTGTTTACCATATTGATCTTTTTCTTGAATACTTTTTTCAATAGCTGCTCGATTATTTGGATTTACTTTTTCTAAATATTGATGAGTATAATCTGATATTTTGCTATTTATAAATTCATCAGGAATAATAATTGTATTAGTATTTTCTAATTCACTAATTAAGCTGTCAAAATATCTCATAACTTCTTTAATGGCATTTTGTTTTTTAGTCATTGAAAAGAAAAAGTTTTTTAAAGTAATTAGATTTGTAACATAAGGTATTTTAAGAAAATAAATATTTTGATTGGTTAGTGGCATCGAAACAGTCCTTTTGATTTCAATTACTTGCATTTCTCATTTTCTACCTAAAGGATCATTGATGCTAAAAATTTCACCATATCTATGATTAAGAATTGAATTATTAATAGTAAAGTTGTTTGTGTGGTTAGCTATTATAAGAACATTTTTATCATCTTTCATTAAGATATTATTTTGAAATAATTTAATGTTTAACTTAACTAAGTCGTTTTGTTTAATAAAATCATTTACTGAATTTTTAAATGTGTAATTTTTAATTGTATTTTCAAAAATTGCACCAATTGAATTTTCATCAAAAAAGTTGTAAATATAATTTACTGGAGTATTTTTATAGTTAATTTTTTTAAGCTCTTCAAAAGGATAATAAGCAACCACAATTTCCGCACTAATAGTGTTATTTGAAATAATATCTTTTCTAATTGCTGGACTAGCAATATAGGCTAAAGCATCACTTTTTAAAATTTCATTTGAAATTAAGTTATATATTTTTTTGCATTTATAATCAAAAGATTTTTCTAATAAAGACATTGAATCAATTTTAGTTCTGTCAATTTTTAAATCTCTAACAGTATTATTTATCATTGCCTCAATTTCATCTTTATTCATTTTAATATTTTCTCTTTTTAATAAATAATTGATCATTCTTCCTCCAAATCATTTAAAAAACCTAATATTTCATTCTTATAATATTTTAAATTATTTATTAAAATTTAATATTAAATAATATATAAACCACATAAAAAATCTCTTTTTAAAATAATTTTTTTACCAAAAAACCTGTTTTATAGTGTTTTTTGTATTTTTGAATTAAAAAAATAAAAATTAAAATATTTCTTTTTTTCTTAATTTATAAGCTTAAAAATTGCTAAAAAAATATTTAAAATTTTTATTTACAAAAAAACTTTTTAATATATAATAATAAGGCATAACTTAACAAATGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCATTTGGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCATTTTGGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCATGCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCATTTATATGCTTTGCTATAGTTAGAAAAGTTTTCTTTTTTTATTTAGATGACTTTTGCAGGTGCAATTCCTTCCAAAAAGCTAGAAAAAGTTGCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCAAATGCTTTAGCACTTAACTTTTTAATAACATCAACTTTGAATGGTTGGTGTTTTTTTATTAATTCAGCCATATCTTTTAAATAATTTCTTATATTCTCTCTTTCAAAAACTTTACGACTTCCAATTAAATAGAAATTGATATTATTGTCTGGACTAATATTTCATTTAATATAAGAATTGTTGCCACAAAAAATATCAATTGTTATATTTTTTTCAAAATAATCAACTTGCAAAATTTTCAAGTTGTGTCTAATTGCTGCATTTTCAAACATTTTAAAATTAATTATCTTATTTTTCATTTTGCTCCTTTTTATTAACTTATCTTTTTTGTTTATAAAAATGCTAAAAACTGGAAAAATTCAGGAAAAAGAGATCAAAAATACCAAATTTTTCCAGAGATAAAGTTTTTTATTTTCTATTAAAAAGGTTATTTTGAAAGGAGATTAAATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGATCTTTATATGGATTTAAAGATTTCAAAGAACGCTTATTATTTATGGACTTAATTAATTTAAATGGAATTGGACCAAGAGTTGCTTTTAACATCCTTAACCAAGGATGAGAAAAAGTTGCATCTTTAATTGCTTTAGGTAAAGTAGATCACCTAATTGAAATTCCTTATATTAATCCAAGAATTGCTCGAATTGTAGTTGGCGAATTACAAGACAAATGAGCTAAAATAACTAATACTCAAGATGCACAAAAACAAGTTAAAAATTCAAATTTATTAAGTGAAGCTAAAGAAACATTAAAAATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGATATCATCTTTATAGATGAAATTCATAGCATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAGTGAATTAATTTTATTTTATTAATATTAAAATTTATATTTGATATATAATTGAAAAGTTATGTGATATAAACTTAAGCCTTTCATGAGAAAAATCTTTAGTTGAAGCAACTGAAAAAGATGAGTTGTTTTAAGTATTACATTAATTTCCACAATCATTGCTATCGTTTGTGGAAGTCTTTTCTATATTTCTAAGAAGCCAAACTTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGATACAAGGCTACTGAATATATTACCAAAAATAATAAAGATCCAAAAAAAAGAAAAATATTGATGTGACTTAATCTAGATGCTTTAGTTAAATTTGCAAAAACTGAATATGCATCAGATTGAAAAGAAGCGGGAGAAAATCCATATAATTTTGTTCATATTGGTAATAAAGCGTTAAATGATAAAGGAAAACCAAACGCTTTAAAAGAATATGAGTTTAAAGGAACAAACTACTTATTAAGTGAAGCTGGTCTTAGGGAACCATTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGACTTCTTGGAATTAGACAAAAGAAAATATTAGCCCCTAAAAAAGAAAATAAATTTAACAAATTTGATTTTGTTAAAAATTCAAAATGATTTGGAATTGGTTCATTAATATTTATTGTTGCAGGAATTATTGTTTTTGCAGTATTTGCTGGAATTAATAAAGATTTCTGAGCAGGATTCAACAGATCTATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGAGGAATTGATCCGAATAAAATTACATTCCAAATAGTGCCTATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGAACTTATTCAATTGCAGCAGTATTTGGCTTATTACATGATGTTTTAATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGACTTTGAATTAAACTTGAAACTAAACGTCAACTTTGTATCAAAAAACGTGCAGATAATAGCTACTGAGTTTTATCTGGATCAGAAGAACAATTATTCCAAGGTATTAATGATTTTACAGCTTAAAAAGAGACAGGCTAAAGCCTGTTTTTTATTTGCCTTCTTATAAAAAATGCTTTTATTTTTATAATTTTTTTATAAAATTATAAGGCACACCAATAAGGAGAAATATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGATTTTTAAATCAAAAAAGCCAAATACCTCCTATTTATAGTGCAAAAAAGATAAATGGTGTTCGTAGTTATAATTTAGCTAGAAATGGCCAAAAGGTTGAGTTAAACACTCAAAAAATAGAAGTTTTTAACTCAAAAATTTTAAATTTTGATTATCAAAAACAAGAGTTAACTATTGAGTTAGAAGTTTCTTTTGGAACATATATTCGTTCCTTAGCAAATGATGTTGGAATAGCATTCAATACTTATTCCTACATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTAATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAATTATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGATATTCACCAAAAATGAATATTAATACATGATTCTTATCACCTCATCAAGCCAAAATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGAGCAACTGGTGTTTTTGTTAGTCCTAAAGGAATAACCAAATCACACACTTTAGATTGATTAGCTAAATATTTAAAACTAGATTCTAAAAAGAATTTAATTGCTTTTGGCGACAGTTCAAATGACTATGAAATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAATTTAATTTATAATTGAAATATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAGTAAAAAAACAAATTACTTTTATTTTGCATTACTTTTTGCATCTAAAATAAATATTTGGTTTTTTATTTTTATTTATTATAATAATAAATATTTATTTAAGAGGAGAACTATGGCTGTTGGAATTATTGCTGAATATAATCCATTTCACAATGGACATATTAAACAAATAAACTGAGTTAAAGAACATTTTCCAGGTGAAAAAATTGTTGTTGTTATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGAGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGAAAATTTCAAAAAATAATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTTGAAGCATGTACTTCAAAAAGATATACAGCTTCAAGAATCCAAAGAATAATAGCTTGAATTTTAAATAGAAAGTGAAAGAAAACTTCAAATCGAAAAGTTAAAAAAATTATTAAAATTCATAAAAAAGAAGAAACACAAAATATTGAATCAAAGAGTAGATAAAATTAATTTTTCTTTTTTATTGCATTTATTTTTTTAATTAGGAAATTCTAATAAAATTAAAGTATATTCAAGTAGAAGGAGTTTTATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGAAAAAACATCAAAGAAATATTTGATGCAGATTTTTTATTAAAATATCGTACTTTGCAAAAAGAAGGGCTTAACTCTATAAACTGTTTAGCTCAAGATTTTATTAATGAATGAGAAACAAATCAAAAAAGATTTGAAACAAGTGATTCGCTATTACCTGTTGATTTATTTAAAAATATTTATTCATATTTTGAAGAAAAACAAGATTTACAAATTCAAATAATTGATAACTTACATAAAGATATTAATCAATTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGAGATCATCAAAAAGAAAATGAAATTTTACTTAAAAATTATCAAGAAGAATTCAAATCTGAAATCAAAAATTATGAATTTGAAATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGACTTGCAAAAAGTAATAAGAATGAAAAAGTTGAAAATACAAATTATGCAAATAATTTACAAAATAAATTAAAAGATAAAATTTTAACCTTAAAAGCATTAAATATAGAAATTAGCCAATTACAAGAAGACTTAAAAACTGTTAAAGAATTTATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAATTGAGCCAAATCACTATTTATATGGAACTAGAAATCAAGTGCTCAAGTGAATTGAAAAATATAATTTAGTTAATCCGGTTTTAGCTCAAGAATTAGAATATCAAAAGATAGCTAAAAACACAAAAGATTCACCTTTTGATGGACTTAATGAAATACCTGAAGGTGTTGATTACACTCAAGTTATGGATATTAGTATGTTCACTTCTGAAATTAAAGTTTCAAAAACAACTTTCGATTTAAACAAAAAAAGTAAGGAAAAATAATGCTTAAGCTTTTTTTTACAAGTCAGCATTATTTTCTTTCAAATTTTTTTAATTTTGCTCGTTAGCAAATTCTTTGTATTTACTATCAACATCTTGAACTTTGTTATGAATATCTTCATACCATTTTTCAAATTCTTTACGATATATTGATGAAATAAAAATATGACCATTGCATTTTTTTGATTAATAATATCAATTTTGTTTATATTTTCTAAAAATTAATAATTAGCTCATTTTTAAAAAGTATGAAAATTAATTCTAAATTTTTTGAATTCATTTTTTTCTAGATCGCTTTTGCTATTAATAGTTTTTAGAAATGTTTTAAGATAACTTTTTTCCTTGTTATATTCTTCTTTAACAACTAAAATTGGCTTACAACTAGCAGCCAAAACTGGAGTTAATAAAGCAAGTGAACTAATTGTATCTAAAATCAATTTTCTTATTCTCTTCATACTTCTATCATAGCAAATATAATAATAAAGGGAAATTTGATATTATCAAATGCAAAAAATACTTGTTATAATTAATAAATCACAATAAGGAGAATTATGCCAATTTGAGGTCAAATATTATTAGTACTTTTTTCATCAATATTTTTATCAACAATTTTTGTTGTTTTAACATATAAATTCTTTTTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGAATTGAATCAAATAAAGGATATGAAGAAGAATTTACTTTTTATAATAAAGCATATGAATATCAAATCGATTTTTTTGATTTTGATTTAAATAGTGCAAAATATATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAGCCCTTACTTTTTTAAATAAAAATCTCACAATTCTTGTGTCATTTTGTCATCACAATTTTCATCATTACCATTTTTTAAAATATATTCATATCAATTTTTATGCAATTGTTGAAAACTTTGAAGTAATCTTGTTGCATATTGATATATTTTTCTATTTCTTAGTGATACTGGACCAGGTTTTAAAGTATCTACTTGAACAATATATTTAGAAAAATTCACTAAACTTTCTCTTAATTCTTCATATTTTTCTTCTTGAATATCTTTTTTATTTTCAAATTCTTTGACACATTCTTTTGATTTCACTATCTCATTATTGAATTTATTTTCAATAGCTTTTAAATTGCAGCCACTTGCTGAAATAGTCATTGAAATTGGAATAGCAAAAGAAGAAACAATTAAAATATTTTTGATTTTTTTTCTTTGCATTTCTAACCTTTTTTTGTTACGAAATTAAGTGTTTTTATTATTTATTAAGATAATATTATAACAAATTTCAACATAATGAAAAGAGTTGTGAAAAACTTATGAGAATTTTTCAAAAATTATCTTAAACATTTAAATTTGTATATTAATAAAATATAATTTTCTCGTGATAAATAATATAGCTCAATATACCATAGTTATCCATAACTATACATTCTAAAATTTATTTTACTTATATTTATTTTTGTTTTATTTGTTATTTAGCAATATTTTTTATCATTAAAATACATTTTATTTGAAAGGAAGTCAAAATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATATAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGATTAATTCCTATTGCTAAAAGCTCAATTAACTTAGGCGCTAATGGTCCTGTTCTTTCATACGTAATTGAAAGTATGCTTGCAAAAGGGGCTAAAATTGATCCAAGTTGAAAAGATAAATGAGATAAAATCAAAAAATCAGGTTTAAAAGATAGAGCAGCCGTTATTGAAAACTGAGGTGCTGTTAAATCAGATGTTAGTGCATTAAAATTAAATGAGTTAACAATTAAAGAATCAACATTCTCAAATATTTACTCATTATTAGAATTTTGTGACAAAGCACAAAAAATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGAATTACAACAGTTGTTGAAGAAGATGGCGAAAGAAAAATAAGTTACAAATCATTTGTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGGTATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGAAATGAAAACAATAATAAATTTGCCCAAATTGTAAAACATGATGATGGAACATATCGTATTGGTGGTCACTTAAATAAAATTAAAAAAGACACTGAAAAACTTGAAAATTATGAATGAAAAGCAAAAACCGCAGATATTTACAACAAATTAGCAGGTTATAGTCAAATTCCTACTGGAACTAAAAAAATTAGTTCACCAAGTGGAACAAATGTTTATGCTTTCATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGATTTGTAACATCAGGCGCAACTGAATTAGAATATAAAGATCAAGAAACTGGAAATGTAATTAAAAAAGTAATAAAACCTTCTTTAATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGAAGAAATGTTTATGGTAACAAAAGCCCAGGACAATATAAAGATCAAATAATTGACGCTGTAGTTGCTTCAAACAACAAATTATTTAATTAATTTTAATTAAAAAAACACAATAAATTAGATAGAAATATCTAGTTTATTTGTATAAACTTAAAAATTTATTTTTAAAAAAGGACTTATTATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGAAAAGAATATGATAAGTATGACATTTCAAAAATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGAGATCAAATGGCAAAAGAAGGTTTAAATTACTATGATGGCGAAATCGTAAGTTGAAATGATGGAGATACTCCTAAAGTCAAATATTATGATCCAAAGAAAAAAGAGATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGAGGCGAAAAAAATGGACACAAGTATGTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAGGGCTTTAAGATTAAATTAATATTATTAAATTAAAGGAAAAAATATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGACAAAACAAAGTTTTAGATAGAAAAACAGAAGCTATCATTAATATTAGAAATATTTATCTTAAATCATTAGGTGCTAGTGATTTAGAAATTAAGCAAATTAATGAAGCTTGAAAAATTTACAAATCAATTTATAGAGAAACACAATCAGAATTAGCTAATTATCGTGTTGAATTACGTGAAAAATTTGATAAAGCGTTTACTGATTATAAAATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAACAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGAATTAGAGATATTCAACAAAAATTAGGTATTACTACAGTATTCGTTACACACGACCAAGAAGAAGCTATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAACTTTTGAGCAAAATTAGCTATCAAGTTTTCTTGATTAACTACTTTTAGTGCTCAAAAGCAAGCTAATAGAAAAGAAGAAGCTCTAGCTGAATCAGTAGTTGAAAAGCGTACTCCTGCTTACAAACCATTGATTTTGTTAGTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGAGCAAGAGGTTTTTGACAAACAATATTCTTCTTACCTTATATAACAAATATTGTTGCTGTGTCTTTATCATTTATTCAATTTTTTGAAAATAATGGTTTATTCAACACAATTTTCAATTTAGGAGAAATTCCTTGATTAAATGATAAATCACCAAAAGCAATTATTTCTTTCTATCCATTCCTTGCAATGGTTGTTCAAGGTATTTGATCAGGGCTAGCATTTAATGTTTTAATTTTTACAACAGCAATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGAAACAAAATTAAAGATTCAGAAGTGATGATCAAATATCAAAATAAGAAAAAACAAAGACAAAACAGCGCAACAAGTTAGAGAAGTCTCTGCTTTTGGAATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGAACAAACTATTCAACCGCTGCTAATACAAAAGCTAATGGTAATAGTGATTATTGATCAGCATTTGCATTAACTTTTGCCAATGTTATTTTCTCGATTATAGTTAAAATATTTATTACATTATTTGCAGGTTATGCCTTCTCGCTTAAAAAGTGAAGAGGTAAAAACATATTATGAGCTATTTTAATGGGATTATTAATTTTACCTGAAGTTGCTCTTCTTTCAGGACAATATAAAGTTGTTACTGAATGAAACAATGCAATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGAAACTCATACTTGTGACCATCTCTTGCTCTTGGAAGCGACTACTTCATTATTTCTACATGATTATTCCATGTTGGTCGTGATGAAAGTAGTGGTGTTCCTATTATTCTTCAAAGTATTAAAATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAGGAAGTGAAATTATGAAAAAAGCAAATATAAAAACCAAAACAATAATTTGATTAATACTCGCTATTATTGCTTTTATACTTTTAATCATATTTTCAGTTGTATTGCATAATACTATTCAAGTTTTAAAATTATCTGAATTTATTAGTTTCGACAAAGACTATCTACATGAAGCTATGTCACAATATTCATTTGCAATTGCAATAATTACATTTAGCTCAATCATAATTCTAATAGGTACTTATATTTCATATGCAGGATTCAAAAGTTGAAAATATAATGCAACTATTTAAGGAGAATAATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGATTACAAAAAAATGCTAAAGATAAAACAAGTAATCAATTAGAATTGGCTTACAACTTTTTTGCTCCACATCGTGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGAAAAATTTTTAGCTTATCAAACTTTTTTGATAACTTAAGAAAAAAATTCGACCCAACTAACCCAGTCGATGAAGAATTCACAAAAGATAAATTAATTACATATGATGCATTTCCATCTGATTGAACATTTGATTTACCTAACACTGTTACAAAAGATGATGTAGATAAAATGTCTATTTACTTAGAAAATGGTGTTAAAGAACTTAAATTTACATTGACAAAAATAAAACAATATTCTAATATCAAATAATCAAAAATAAATTAGGCCTTGCGCCTAATTTTTTATTTAGCCCTGTTTTATAGTGCTTTTGGTAAAAAAAGCAAACTTATTTATTCTTCTTCTTTATCATCATTTTCATCACTTAAATCTTCAATCATAGGCATATTCATTGCAACTTTATCAATGTCATAATCTTCTTTTATAATACCTTTTTTTCGTGCACTATCAATAGAATTTTGAATTCTTTTCTTTACTCTATCGACTATTTTTAATGCTCTATTTAATTCAACTCCTGTTTTATCATTTTTCTTGATACTTTGTTCAATTTTGGTATAAATATTATCTTCACTTTGACGAATAAATAAGAATGCATCTAAAAGAATGTCAATATTTTGTTTTATGTTAATTCCATAATTTGCATAAGCAATGGTTTGAATAATCATCAAAATTGTACTTGGTCCAGCCAACATAATATCATGATCTTTATTCAATGCTGAAACCAATTCATTGTCACTAACTGCTAAAGCAAAAATAGCTTCAGATGGTAGATACATAATTGCTTGCTTGGTAGTTACATTTTTAATAATGTATTTACTTTTAATACTTTTAGCTTGTTCGAGAATAACTTTTCTAAAGTTAATTTGAGCTTTAATTCTTTCTTCTTTAGAGTCTGCATCTTGATATGCATTAAAAGAATCAAGTGGAAACTTACAATCAATAGGCAAGTAACGATCATTTTGAGAGTTAGCTTCTCCATTAGGCATTTTAATAACAAAGTCAACTCTTTCGCTATTTGGGTTCATATCTTCAAAATCAACAGCAGCAGCTTCATCTAATCCTTTTTTAGCAATTTTCTTTAAATCAACTTGTGCAGCTCACAAGTCTTTTGATAAATGTTCATTCAAAATACTCTTTAAATTAAACTCACCATAATTACCGCGACTTTTTGTTCCATCAAAAGTTTTTTGTAATGAATTAACTGAATCTTGTAAGGTCTCAAAACGTACTAAATTCTTGTCAAGATTTTCCATGTTTTCTTTAATATTTTTAAAGTTTTCTTCCATCTTTTTAGCTACTTCATTTTCAAATTTTTCATCAATAGCACCTTTAATTATTTGCACTTCCTTAATTACTAAATCATCAAACGATTTCATTTTTTTGTCAATTTCTTCTCTTAAATCACTAAAGTTTTTATTAATCATGTTGAATGAATTGGCAATTTTTTCATCAACATTTTTATTGAATTTGACAAATTGACTAGACAAATCTTCAGTTATTTGATTTTTAATATTATTTGTCATTTTGTCTTGATCGATAACTTCTGTCTTATTTTTTTTATATAAGATGAAACTAACGACAATACTACTAATAGCTAAAATCATAGTCAATATTACTACAATTAGAATTCCTATTTCCATATTTTTTCTCCTTTAATTAATTGGTTTTTTAAATTATATAATTTTTAATTATATTTTGATTAAAATAACAACTTAAAAGCTCAAAAATTGCCACAATAAATATTATTTTTTTGTTTTTGCAAGACTCAAAAAGTTTTACAATTTAAAATTGTATTGATTTTAAAATCTAGAAAGAATTTATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGATTAAATAAACCAATTTGCTATGGTGCTTTAGATGACCATAATAAGTTGCTAGGTTTTGCTGAAGGTTATTTAGAAGAGTGAAATAATCGTTTTAGAATTACTAACATTTTAGTATTTGATGAAAATAGTAGACATAGTGGTATTGGTTCTAAATTAATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAGAAATTTTGCTATATAATAGAAATATAGTTTTTGGAGGTAAGATATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGAGTTTGTGCTATTTGTGATGCGCCTAATGTTCTTTATGAAAATCATTTTATTGATGATAAGGTTAAATACAGTTCATTTCCAATTATACCTTTAGTTGGCGGTAAAAATAGAAATGAAAAAATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAAAAAGGCAATAAAAAAATCAGCTTTTGAAAGTTGATTTTTTATTATTCTTCTTTTTCAATTTTATGCTTTTGAGCCTTTTTTATAAGCACAAAGAGCAAAATTTGAAGTTTCAACTTATCTCATCAAACAGTGTTCGAAATGTTTTCGAAATTGCAACACATTTGATATTCAATAACCAATTTACCTTTAACATCATCTCAGTAAAAAATCATTTGATGTTTCTTATTGTTAATTAATTTAAAAAGCATGTATTTATGATCATTTCATACTCGATATGCATTAAAAGCTGTAAATCATCTTTTGTAATTTAATGAGAAATATTTAATTAAATTTTCTAGTTTATCAAAAGAATATTTGTATGATTTTTTGCTAATTTTAATCTTGCCAACTCTTTCAGCTAATTGATTGTATTTGAATGAAAGTAAGTTGTTTCGATTTGTATAACTATTTAGCATTCAAATTAAACAAATGAAAAAGTAATAGTTATTAAATTGAGTTGAATAACGAGGATTAGCTATAAATTTGTTATCAATATAAGCAAATAAACAGCTTTCATTTTTTAATTGTTTATTTATATTTTTTAAATCATAGAAATAATATTTAACTTTATATTGTTTTAATAATTCTAATATTTGATAAGTAGTATTTTGTGGAACAACAACATAAGAATTACTAATATCAATTCCATTACGTTTGATTTCTTCTAAAAAGAAATCTAAATAAATTAATACTAGTTCATGAGAATTAAGTCATTTAAATTTGTCATTTAAACGCAAGCAAACATTTAAGTTGCTGTTGATATCAAAATGAAAAATATTTTGAATTTTGTTTTCACGATATCATGAGCTAGCTGCAAAAAATGTTAATCATTTTAGGTTTAAAAGCGAAATATTTTTATTGTTTGTAATGTATTTATTTTGATAATTATTTAATAGTTCTGTACCAATACTTTGACTAAATTTATTGTCACTAGCAAAGTAACTTAATGTTTTATATCTTTGTTTAACATTAACGAAAGCTTTAAGAATTTGTTCTTTTGAAGCTAATTCATCAATCAGTTCAAATTCATTAACTAGTTTAGGATCTTTGATGACAATTTCTTTATTTTGCCATTTGTTTAATTGATAATAATCATAAATTTTTTGTAAGTTTTCAGAGGTCAAAATTTCAAAGTTTTTATCAAATATTTGAATTGTAATATAGTTATCATTTTTAATATTTTTAAATTTGATTAAAAAGTCATATGAAGCTTTTTTATAGGCATATTCATCTAAAATCAAACTAATTGAAAAGTTATTGTGATACTTAAAAACATTATGATTATTTTTGATAAAATAAGAGCTAAAATATTGAATATTTTCATTATAAAATTCTTGACCTTCAAAGCTTAATAAAATGTTACTATTGCTTTTTAAAATAGTTTTATTTATAGCTTGCAATAGTCGCAAGATTGATGAGAGTTCTATTTTTTCTTTGTTATTTGTTTTAAATAAAAAGGTATCATTGCGAATTAAAAGATTATCGTTTGAAACGATAATTTGCTCTTTTAATTGTTGATTATTTGTTTGCATAAAATTACCTATTATTTATATTAAAGATTTTTGATTATTCTTCATTGTTTGAATCAATATCAATTTCATCGTTTGAGTTATCATCTAAGTTGATTTCTTGACTTTGTTCATAAATGCTTTGAACAACAGAAGTTGCAGCCATAGTTGCTTCTGGATCTGTTTTTTCATCAATTTTAATGATTTCAACAGCTTGAATTTCTTCACGCTTTTTAAGGTTAATTATTTTAACACCTTTAGCTGCCCGACCAGTTTGGCTAATTTGGCTAATAGCTGTTCTAATTGTTAAACCACTTGTTGTAATAATTAAAAGTTCATCTTGAGGATTTACGAATCTAGCAAAAACAATGTCGCCTGCTAATTTGCTATTAATTCCTTTAACACCTTTACCACCACGTTTAATTAATCTAAATAGTGAGTGGTGAGTAAGTTTTCCAAAACCTTTTTTACCAATAGTTAAGATTAATTCACCTTCTTGACTTGTTGAAGTGCTTGAAACTGTTTGATGGCTATCAAGTGTTATACCTTTAACACCAGTTGCAGTTCTACTAATTGGTCTAAAGTCAATAGCTTGGAATTTAACAATTCTTTCTTCATCATTTGCTAAAAGAATTAAGTCTTCATCAGTAATAATCATTGCTCTAATTAATTCATCGCCTTCACGTAAGTTGAAAGCATATTTACCATTAGCGTTAATTCTTTCGTATTCAGTTAATCTAGTTTTCTTGATAATACCATTTTTAGTTGCTGTAAATAAGTAGTGATCTTCATCGTAAGTGTCTACTGGAAGCATTGAAACAATTCTTTCACCTTCTTTAACACGAAGTGAAGGAATAATATTAATGAATGCTACACCTTTACTTTGACGAGATAATTCTGGAATTTCATGAGCTCTAACACGGTAGACACGAGCATGATTTGAGAATAATAGTAAGTCGGTGTGAGTTGTAGTTGTTAATATTGAGGCAATATCATCATCGTCATATGTTTTCATAGTACCAATACCAACTCCACCACGACGTTGAGTTTTGTATTCTTCTAAGTCCATTCTTTTTACAAAGCCTTTGGCGCTGGTTGTAATAACAATGTCACGTTGAGGAATCAAGTCTTCATCAGTAATAGTACCTTGAATTTCATAGTTAATTTGTGTGCGACGTTTGTCATTGTATTTTTCTTTAATTTCAGTTAATTCTTTAACAATTAATTTAATTAATTCACTATGGTTAGCCAAAATAAATTCAATTCTTTCAATTTCTTTTCTTAACTCTGCCATTTCTTCAAGCATGTTTTCAATAGCAAGTCCTGTAAGACGACCTAAACGCATGTCAACAATAGCTTTTGTTTGTTTTTCAGTTAAATTAAATCTTTTTGCTAAGTTAGCTTGAGCTTCACCATCTGTTTTAGATTTCTTAATAATTGCAATAACTTCATCAATATTTTCAACTGCAATTTTAAGACCTTCTAAAATGTGAAGACGGTCTTGAGCTTTAATTAAATCAAATTTTAATCTTCTAGTCACTACTTCTTCTTGGTGTCTTAAGTAAACTTCTAAAGCTTGTTTTAAGTTTAAAACTTTAGGTTCACCATCAACTAAAGCAACAAAGTTAACATTGTAGTTTACTTGTAAGTTTGTTTGACGATAAAGCTTGTTTAATAAAACATGAGGATTAACATTCTTTTTAACGTCAATAACAATTCTAATACCTTCACGGCTACTTTCATCACGTAAGTCGGCGATACCTTCGATTTGTTTATCTTTAACTAATTCAGCTATTTTTTCAATAATTGCAGTTTTCTTAACTTCATAAGGAATTTCAGTAACAATAATTTTGCTTTTACCATTTGAGTATTCTTCTATTTTAGCTATTGATCTGATTGGAATGGATCCCTTGCCTGTTAAGTATGCATCTTTAATACCTGCATTGCCTAAAATCATAGCTCCAGTAGGAAAGTCTGGACCAGGTAAAAATTCCATTAAGCCATAAATATCAATTTCTGGATTGTTAGCTAAAGCAATAGTTGCATCAATAGTTTCACCTAAGTTGTGAGGTGGAATACTTGTTGCCATACCAACAGCAATACCACTACCACCTGAAACTAAAAGGTTAGGGAATCTTGATGGTAATACTGAAGGTTCTTTTTCACTAGCATCATAGTTATCAACAAAATCTACTGTATCTTTTTTAATACCTTCAAGCATTTCAGCAGCTAATTTGCTCATTCTTGCTTCAGTATAACGCATCGCAGCAGCTTCATCACCATCAATAGATCCAAAGTTACCGTGACCATCAACTAAAGGATAACGCATACTAAAATCTTGCGCCATACGAACCATAGCTTCATAAACTGAACTGTCGCCATGTGGGTGGTACTTACCTAAAACGTCCCCGACAATACGAGCGCTTTTTCTATGTTGCGATCCAAATGTAATTCCTAATTCATTCATATCAAAAAGAATACGTCTATGTACTGGTTTAAGTCCATCACGAGCATCTGGTAAAGCACGAGAAACAATAACACTCATTGCATATTCTAAGAATGAGTTTTTCATTTCTTTGTGAACTTCAACTGGATGAATACCATCTTTAGGTGCATCTAAAAGTTGTGGTTGAACTACATAGTTGTCTTTATTTTGAGGAACTTCTTCTTCCTCCCCTTCAATAACTTTAGTTTCTTCTTTTTGTGGTTTAAAAGCAACAAAAAGCTCTTCATCAGGAATGTAATCTTCATCTAATTCTTCGATTGCATTTTCTTTTTCTTCTTCTTGATTAAGTTTGCTTTTGTATTCTTTTTCTTTATCTTGTTCTTTTGAACTCATAAAATACCTCCTTCAAACTTTTAATATATAAATATATTAAAATATTATAGCATTAAAATGCAGTTTTTTTAATAAATTTTTTATTTTTTTATTTTCATTTTTTATGGCTTTTTTACATTAAAATTTTAAAACTATATTTTTACTAAATTAGCTGTTTTATAGGCTTTTAATAGAAAAATCAATATAAATTAAGAGAAAAAATTAAAAGCTAATTATGTTTGCTTTAATTAAAATAAAATATAATTAAATAGTTTAAATTATAGAAAGGATTAAAAAATGGAAAATATAAACACAGAAAAACATAAAAAAATGGACTTCAAAAGTTGATCAAAATTGAGAAAATCAACATTTATTGTGGGTATTATTGTTCTAGCAATTTGTGTGTTAACTTCTACTTTAAGTTGAGTTAATATCTCAATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGAAAAAAAACTCAAACTTTTACTTATATTTCTAATTATTTTTTAGCTATAAGTATGATCCTTTTTGGATACTTTTATCACAATAAAAGAATTCTAAAGACTTTCTTTTTAAGTGTAGTTTACATTAGTATTACTTTTGCTATTTATTGAACTTTAATTTTTCCAAAAATATTTGGATCAGAGTATGGTTCCTTTAGATTAGCTTTAAGCACAATTGTACATGCAATTAATCCATTAATTGGAATAGTCATATTGATTTTAAATCGTAAAAATATTACACTTGATAAAAAAGTTATGTACATGTCTAATTTAGGTGTTATTGGTTATTTATTATTTGCCTTTGGCACTTTTTGAATTGGACTAAGTTATTCACAAAACTACAAAAATGATGTAGCAAATAAACTAAATCAATATGTGGATAGTGATATTGTAGTTTATGGCTTTTTAAACTTTATTGAACCATTTTTTTATCGCGGAGGTAATATTGCAATAGTTATTACATTAGACTTATTAATGATTATAATAGCAGTCTTTGTACCTATTGGCTTAAGTTATTTTTGAAAAGCAGTATTAAGAATAAAAATTGAAAAAAATAATGAAAAAGATAGTTTATTAGAAACGAAAAGTGATGAAAAAATAGCAGAAGATATTAATTAAAAAAATATTAGGTTTTTAAAACTTTTAATATAAAATTAAAGAGCATATAACAGCAAGTATTCATAAAAGAAAGATAATAAAGTTTCAGATTCGATTTGAAAGTCGGTTTGCTGATTAAACCAAAATCATTAGAACTTTATTAATTCATTTAGAGAATACTCTCTTCTTGTTTTATGCAGAAAGAGGAAACTTATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGATTAACAATCAAAGGTTTTGAAGTGTCATATGACCGTTTACCAGACAGAAGTGAATTACATCCAGAAATTAAAGAAAACTTTATCGTTGAGTTGTACTCAAAATAATAATTAAAGGAGTAAGCTTATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAATTTTACTAATAAGAGTTTTTAATACTCTTATTTTTTATTTTTGGCACTTAAAAGATTTATTGACATATAAATTTATTTAAAAAATTACCTAAAGCACTATAAAATAGGTCTTTAAAACACAAAGTTAATTTAGCACTCTTTTTAATAATTACTATTAATATTATTAAATTAAGATATAATTAAATTATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGACAAACAATTGAAAGTGAAATTGATGAAGACGAAAATATAAAAATAGCAATTTGTGATGACCACAGTACCTTTATTAGTAAGAAACTAGAAACACAAAGCAAAATTAAAGAAATAGCACTTGTCGGCACTAGCCGAATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACGGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAATTCAAAATAGGTCAGTGATTAAAGTCACTACTTATTTTTTATTAAAACAACTATAAAATAGTTATTTAAGTAAATACAACTTTAATTTTTTTAATTATTTTTCAATTTTTTTATGAAAATAGTATTCAAATATATTAAGCACTAATAAAAATAATATATAATTAGATTATGTTTAAAAAATCATGTAAAGATGCTTGAATAGTTGTCAAACGTGGCAATATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAAAACAAGAAATTGCAAAGTGTAGAGTGAACCCCAAATTCCGAACTTTTTGGAAAGGGGTTCATTTTTATGTAATTTTAATTTAAAAAAAGAAACAAATGAAATAGATATATAAATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAATAAAAGCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATTCAAAGGTGATGCTCATAGAAATATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGGAACAGATGTTACCGAATTTAAGTTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAACGCAATTTAAAGCATGCAAGAAAAGGAAATTGTTTAGACAATAGTCCTACCAAGTGTTTATTTGGTGTTATAAAAATAGAATTTTGATTTGGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACCACTTTAGAAGAATATATTTCATATTATAACAATGATAGAATTGTTAATGAATTAAAAGGACTTAGCCTTGTTCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATGTTTGAGGTTCATACTAGTGTAGCTTTGTAATTTTTTTACCTTTTTTCACAAATTTTTCCATTTTCGCCTTTATTTATTACCAAAAAGAAAAGTAAAAAATGAAAGGAGGACTATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGAGATAATTCTCCAGCAACAAATGGAAATATCTTTTATGCGTGATAAAAAAGAGCAGGGCAAAAGCCTTGTTTTTGCTTTTTATTTAGCTAAAATTATTCAAAAAATATTTTGTTCATTATTTTAATAATGTGGTAAAATGAATAGGCAATATTTAATTGCAGAAAGAAGAATCTAATTCTCACCTGATGGCCAATAGCCTTGGGTTTATGCTACAATTAAAACCTAATTTTAAGTTTTATGTAGTAGAGAGTGGGAAACCACTTTTACTTTTATTTTTAAAAATTTATCTATAAGGAGTTTATTATTCAACCACAAGGAAAAATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAAAATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAATGCTTAACTTGAAGTTATTTAATTTATAATGAAAGGAAATTATCATGAGAGTTAAAGGCGGAACAGTTACAAGAGCAAGACGTAAAAAATGACTAAAATTAGCAAAAGGATATTTTGGCCACAAATCAATCGGTTACAAAGTTGCAAAACAAGCAGTTGTTAAATCATGAACATATGCTTTTAGAGACCGTAAACAAATTAAACGTGAATTCAGAAAATTATGAATCGCACGTATCAATGCAGCAACTAGAGCTGAAGGTTTATCATATTCAAAATTTATTAATGGATTAAAAAGAGCTAATGTTACAATTAACCGCAAAATGCTTTCAGAATTAGCTATTAGCGAACCTAAAACATTTGCAATGTTAATTAAAATAGCTCGTGATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCCCAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAACAATTGAACTAAAACAAGAATTTAAAAAGCTATTTTCTATTAAATCAATACTAGAAAAAATCAAATTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATGGTGAAGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGCATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCCGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGAAATTGATCTAAAATTTCTACAATGCTTGATTATTCTCTTGAAATAAGAACGCTTATTTATACCACTAATCCAATTGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAAGGGTTTTCACATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTGATTTTGAAAATGAAGAATAATTTTTTTAAATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTTAGGCATTATCTTATTTTATTCTGCAATATTATTAGGTGTTGATCTAAAGTTAATTCCTTTCTATTGAGAAAGGGAGAACTTTACACAAAATTCTAAACAATCTTTATTTTTTTAATTCAAATATTAAATTTCATTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTGCAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGACGAATTATTTTTCAAAATGTATTTCTCAGTGATTGCAAATTCTGATTTTCAGTACATTCTATTTCATATGTAGTATGAAGCATTAAAGTTTCCTAATGGGTTATCAAGAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGTTCACCATTATGTTTTAATAAGAAAGTAGTTGAAGCATGATCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGCACTATCATATTTTTCATTTGAATTGTAAGCAGATGATCTATAGTAAGGACCATTGAAATCACTTTCAGTTGGATTTCCATCTGGACCTGGAAGAGTGGGGAATGGTTCTTCTTCGCCATGAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTTTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTATTGTATTTAATGAAATCGAAGGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCTTTGAAAATCATTTTTAAACATTATTTTAGAATCTGATGTTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTCTTCTCATATTCTATTAATTTGGCTAAAGTATATAGATCATCATGTCCACTTCAAATATCATTTTGTATTGCTTCTAAATAATCACGTGAATTCATATTAGGATATTTTTGATTTATATATTTAACTGCTGGTGTGTTACCAAAAAGCCTACGGTTAATAATTGTATGTCAAGGCACATAATATTCATTTTCAATTTGTTCACCTTGTTCAATTTCACTAGTACTATTTGTTATATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTTCAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTGCCTAGAGCTTTAGGTGTAGTTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTCTGAGACAGACGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTGAACGGCTAATAAATAATCATTTTCTAAATAATTTTTAATTGAATTAATTTTTGCAAAATCAACGCCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACAGCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAATAACACTAAAGTTTTCTGTAGAATTAACATTTTTATAAATTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCTACATTTGCTATTGCTGCTAATTTTGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAATAAAACCATTGATTAAATTAATTTTTTCAAATAGTCCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTGTTTTCAATTGAAGATATTTCTTTAGTTGATAAATCTGGTTTGTTATTATTAATATCTTCAATTGTTTTTTTAGCATCTTCGAATTGGTTTAATGTTTTTAAATTAGCAAAAACATTTGATAAGAAATCAGCAATTTTTTTATCTGCATTGCTAATAAGTTTTGAAATTTCCTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCATTTTTAATAATTTCGAATATATCTAAATAATTTGTTTCATATTCTTTAAGATTTGAAATTACTTCATAGAATGTTGAAATATTATTAATTTTGTTATCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTTGTTTGTATATCAATTATTGATTTATAAGAAGTTTCGAATAAATTAAATTCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAAGGCTTTTTTAAATCCATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATAATCATTAATTAATTTGTTTCTACTTTCAATATACTCATCTCATTTAAAAGAACTCAATTCAATTTTAGATTTTTGTACAAATTGATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTAAATTGAATCAACATTTGAAATATAAGCATTTAAAAATTCTTTAATAACAGACTGAATAGAAGTTGTCAAATCATTTTCAGCGTTTAGAATAAATTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAATAAATGAGTTAAATTCATTAAGAGCATTAACAATAGGCGTTAATGATTTGCTGCTTAAGTCTAAAATTTGAGATTTGTTAAATAATTTTGTACTTTCAATTGTTTTGACAAAAGTGTTAACATAACGATTTATATATTCCTTTGTATTTCCATATGATTGTTCTAATGTTTTACATGCCTTAATAATATCTTTTATAATAACACTTCTATTTACTAAAACACCACCATTTTCTAAAATAACGTCATAATTATTAGAGTCAAATCTTGATAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTATCTATGTTTGACATGAATTTTTTAATTAAGCCGTCTTTTTTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCCTTATTAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTACTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAGTGTTTTGATAATCTTTAATTTTAAGTTCTAAAGAATTTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCAGTTTTTAAAGTTAATAAATCATTACTACGAACAACAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTACCATTTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTATGTTTAACAGTTCTCATATAACTTTTGTTACCAAAGTGGTGACCAAGATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAGTCAATTTGAGTTTGACACAAACTTAGTTCATTTCAAAGACTCGAAAGTCATGCTTCATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAGGTATTTAATATTGTTTTCAATTCACAACTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCACCAACAATCCCGGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAATATAAAAAATTAAATTTTTGGGACATAAGGAAAATTATGTCTTTATATTAAAATATTTATTAGACATTTTTGGAGGTCAAAAAACATGAAAAAGAATAATAATTTTCTTGATATTTTTTTGAATAATATTTACTCAATGGTTCAAAAAAACCAAAGAAGTTATCAAACTTTTAAACAATATAAAAGGTATTTATCATCCTTAAAAAATTGAAACAATGCGAAGCAATTAATTGTAAGGATGAATCGTGAATTAGCAAATAATAAAATTTCAAATAATACTAAATTAACTAAAAGAAACATATTTTCTTCTTTTTTAAATTGATTTTGCAAAATCACTAATACAAATATGGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGGCAAAAGACAAGCTATACCTGAAAAGGAATTAATTAAACTTGTTGAAGAATTAAAAAGCTACAAAAACACCAAATTTAGTATCATTTTTCGTATTTTTCTTTCAACTGGTATTAGAGTTGGAGAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAGAGAATTGAAGTTTTATTATTAAAACATTAAAAAACAACAATCCTAGATACTTTAGGATTCCATCAAAATTAGAGATGCCTTTAATAGGTAATCTTAGAGAAGATATATTAGAAAATATAGATAAATTACGTATTTCTGAAAAATCAATTAAAAATATGTTTTATCTTTTTAAAAAACATATTAGTGAAAAATATCCTGATTTTAAATATAAAATCTCGGCTCACGTCTTAAGACATAATTATGCAACTAAAGCTTTTTATAATTTAAAAAATAATGATTTAGTAGCCAAATTATTAGGACACACAAATTCAAATATTACTCAAAAAACTTATATTGATTATGATAAAAGTATCATGGATGAGTATGCTATTTTAGCCATACAAGATCAAAAAATAGGCTTAAATTCTATGCAATTATTAAATATAAATAATAATTTAGAGCTTACAATTATCAAATTAGAGAAAGAAAATTGCAGCTTAAAAGAAGAAATTGAGATGCTAAAAAATAAAATAAAAAAACTAAAAAAATAATGTTTATCATATTTAAATAAAATTTTAAAAAATTCTGCATTTTACTAAATCCATTATAAAATAGCCACTTTGGTAAAATCAAATTAATAAAAATTGCTATTTAATAATGATAAAATAAAAATATTAATATTTATTATTAATAAAAATAATGCTATAATTATATTGAAATTAATTTAAATATATGTTTGCATTTTACTTATGCAACTATAAAAAAGGATTTTAAGGACATAATTATCGACATAATTTTCCTTATGTCCGGAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGAATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAATATAAAATACCTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAAAATCAAGCATGACTTTCAAGTCTTTGAAATGAACTAAGTTTGTGCCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATATTGGTCACCACTTTGGTAACAAAAGTTATATAAGAGCTGTTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAGGAATATCAAAACACTTTAAGTAAAATTGAAGAAGAAGCACTTTCAAGCAACATAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCCGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAAAAAAGATGGTTTAATTAAAAAATTCATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGCGGTGTTTTAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTTAAAACAATTGAAAATACAAAATTATTTAATAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTCGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCAAAACAAATTATTGATCAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGAGATAAGTATATTGAAGGCAGAAACAAATTAATTAATGAATATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATGGATTTAAAAAAGCCTTTGTTAATATTAAATCAAGATTAAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAACATTCTTTGAAATTATTTCAAATCTTAAAGAATATGAAACTAATTATTTAAAAATATTTGAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTCAATAACTTTGCTTCGAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCGAATCCAAAAATTGCTGATTTCTTATCAAAAATTTCTGATAATTTAAAAACATTAGACCAATTCGAAGATGTTAAAAAAATAATTGAAGATATTAATAATAACAAACCTAATTTATCAACTAAAGAAATATCTTCAATTGAAAATAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTATTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAACGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTATTGTTCAAGCTGATAAAGACAATAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCGAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGATTGCAATTAAAAATAATCAAATTTCTAAATTAGATAGTCAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAACATTGATTTAATTAAAAATTATTTAAATGAAACAGATTATTTATTAGCTGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATTTGACTCACAAAATAATGTAAATTGAGATGAAAAATCAAAAATTTGAACTAAACCTAAAGTTCTAGGTAAAAAACATCTATATGACCATATTTCAGATTTAGATACTAGAGGATACTCAAAAGGTAACACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCCGAATATTCATGACACCGATATGGAGTAGATAAAGGAAATACTACTAGTGAAATCGAACAAGGTAAACAAATTGAAAATGATCATTATGCCCCTTGACATACAATTTTTGAAGTTCAAGAACTTGGTAAACCGGGTAAACCAACAGTTAAATATATAAGTCAAAAATATCCTAATAAATCTTATATTGATTATGCAGGACCAATACATGATGATATTTGAAGTGGACATGATGATCTATATACTTTGGCCAAATTAATAGAATATGAGAAAACAATAACTGAAAAAATGCTTTTAAATATCAAAAAAATATCAGATTCTAAAATAATGCTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGAAAGATGAGAAAGTTGAAGCGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTTGAGTTTATAGCAAAGAAAAACCATATAAAACTTTTAAAACTCCAGGTAAACAAAATCCAGATGAAGAAATAATAAATGGATTCAACGGTCCTCACTATAGATCATTTCTTGGAAAATCAGATGAAAAATATGATAGTACTAACCCACTATTTGGATATATGCCATTTTCTAATAAATTATTCTCATATTTAAGAACAGTAGACCATTCTTCAGCTCATTTCTTATTAAAACATAACGGGGCTCCTATTTCATGAGTAAATTACATCTTTGAATTTACTCTTGATAACCCACTAGGAAACTTTGATGCTTCATACTACATATGAAATAAAATGTACTGAAAATCAGAATATACAATCACTGAGAAATACATTTTGAAAAAATCATCGCCTTCTGAAACAGTTTCAATTTGAGATAAATGATCAAATATCTTTACTGCAACAAGTAATAATACATACAGCAAACTTATTTGATAAATTAATGATATTTAATATTATATTGAACCCAAAATTCCTTTTTCAAAAAAAAGGGGGTTCATTTAATATTTAAATTAAAAAAATATTATGACTATTCCATACAAAAAGTATTATTGAAATTGCTTAGAATTTTGTGTAAAATCTCTCCATTTTTCAATAGAAAGGAATTGACTTTGCGTCAACACCTAATAGTATTGTGGAACAAAATAAGATAATGCTTAAAAAACATTATCTTATTTTTAAAATTAAAATTTTAAAATAGGAAAAAGATCTTGCTATGCAAGAAATCTTAATTCCTCTCCACTCTCCCCTTTATTCAATTAAAGGAGAGACCTTCTTTGAAACTAAAGAAGCAAAGCTTTTAAAATTTGAAGAAAAATTATTCTTCATGGGACATCACACAAAATTTTTTACAGTCCCGGTTCACATACCATTTTAGCTTAATTTTAAATAAATAAAAACTTGCACAAATTGTGCAAGTAAATTTCTAATAAATATCTTTGCTAACATCTTTTAAGAAGAAACGATAATTAGTTGTACTTGTTATGTAGTAATCATGTTTTTGATTTTCATCATAAATAGCAATATTAAATGTAATATGTGGCTTTTGATCAATTGAACGACCTACTACATTTTTATTTCCAAAATAGTGCGTATTAGGATTATTTTTTAACGTTTGTAATTTATTGTTTATTTCTTCTAATATTTTTTTAGCTTCTTGTTCTTTGCTTGAAGCTGAATTCATTCTATTTGTAATATTAGAAACTATTTGGTTATATTGTTTTTTTGCATTATCATCAATTATGTGATTTTTATAAGCAACAAAACTTTCAATATTTAAACAATAAGTATATGGAGTATTTGCTAAAAGAGAATATCTAGAATCTAATACATGTTTGAATGTATTCATGATATCTACATATGAAGTACTTTTGTTTGTTTTTGAGAAATGATATTTGAAAATTGGGAAGTAATATTGATGATTTATACCTTTATTTTGTCAATTTCCATTTCACATATACATATTTTCTGAGTATAAAGTATCAGCCATGAATTGCATATTTTCAGCTACAAATTCAATAAATTGAGTGTTATAACGTTTATCATATGCATAACCTGGACAAGGAATTCCTACTGTACTTTCATTTTCATAGAAACTATATAAAGTTTGTAATCTATAACTATATCTAAAGTTGTTTTTAGGTAAAATAATTGTTGTTTTTTGACCTTTAGCATCTTTAAATATTGTTTCATCTCTTAATAAAGGAATTACAACTTGTTGAGAAAATAAATTGCCATCTGGAATAATAACTTTGTTGAAGTTAAATAATTCACCATATTTAGCAACACCACTATAGCTTAATTCTTTTGCAACTGTTGAATCTGTAAAATATTTATTAACAGCTTCTTTATTTTTAACATTTTCGCCTCTATTTCAGTCAAAAACATATTTAGCAACTTTAATGTTTTGATTTTTTGATTTAAATTCATTTACATAATTTAAAGCAGTAAAGTCAAGCGTTTGAGTTTTTTCATCAAAAATATATGATCATAATTGTAAACCACTATTAGCTGCATAAATTGAATTTTTGTCATTAAGATGATCTTTAACATATTGAGCAAATCCACCAACTAACATAACTGTTCTATAACCATCATCAAATTTTTGATTTGAATCATTAATAGCAAGCATTTCTTCATCTAAAGCCATGTCATAACCCATTAAGATATTTCGTTCTCAATCAATTTTGTTATTGTTATGTCTCATTCTACGATTTCCAGGATCTTTACCTAAATCTCTATATAAACTATCCATTACATTTGAATATTTAATGTATCGAGCTAGGGCTACATTGCCAAAGGTTTCAGCATGTTTGTATAGAGTTTCAATACTTGCATATCATCTATATTTATTAATTTTTTCATCTTCATTATTGAATGATACTTGACCTAGTAATTTACCATTTAAATCAACAAAGTATTTGTGGTAGTTATCTACTAATAAACTAGTCATTTTAGCATTATTTGATGAATTTAATCTTTCAAAGAAGTATTTTTCGTTTAATTCTTTATATTGACGATTATGCATGTCAATTGAATTTTGAATTTCAACTAAAGCTTTATTTGGCCCATTAAATGAGTTTTCAACAAATAATTTGTATTCTTTATTATGTTTAGTTTCAACATCTTTAGCTTTATATCTTGCTTCATAATATAATTCAGTTAATTTTTTAAGTTCTTCAAAATCACCATTTTCAATATCATCTTTAATAATAGAATAATCATTTATTAATTGTTCAACATGACTTAATAAATCACCTATTTTGTGTTTATTAACAGTTGCTAAATTAGCAGGATAATTAGCATTAAATTTATCTAAAGAATATTTATTTGTTTTTAAATTGTTTAATACATCAATTTCTCTTTGGTTTACAATGATATCATTTTGAGGATCACTAGATAATAATCAAGGTCTAACATTTGCTACAACAGGAGTAACATTTAGACTCATTAATTGTTTATATTTATTCAGTGTTTGAGTTCTAAATTCATTGTAAGTATTAAGTGTTTTTTGACTTAAGTTTGCAGCGCTATTTTTTACTTTATCTTCTGTTTTATAGGCTTTTTTGCTATTTGCAATTAAAGCATCAACTTCACTAATTTCTTTATTAATGTCATTTAAATTTGTGCTTGCAAATTTTATTGAAGCATGTTCATAAATATTTACTAATTGTCTAAGTTCTTCAACATAGTTTCTTAATTTTTCAATATTTTGTTCATCAAATTCACGCAATTTATTTGTTATTATTTGATTATTATTTGACATTAATTCTTTAATTCTAGCTGTTTCAGTTTCATATCATTGAGCATCTTCAGGAACATTTCCACTAATTAATTCACTTTGTTTTTTGTTTTTGAAATTGTTAAGTATTGTTTCAATAGATGTTTTTCCAGAAGCTAAGTTTTGTTCTTCTTTTTTGAAGTTATTGTAAGCTTCAACATCTTTGTATCCGCTAGGTTCATTGTTATATGAATTTTCAATTGCTTGAATAGCTTTTTCAAAGTTTGTTGCTTCAACAACATATTTAAGGTATTTACCAATTTTTATCATTTCACTGTCGGCATTTCATTTATTTTGAATTGCTTGATGTTTTGTTGTAAGTGTTGCAACATCAGCATCATAATTAGTAATATTTTGTGAACAGAAGTTAACTATATCTGCCTTGAATTCATTAAATTTAGTTGTAACTTTATCTTTTAATTCTTGATTAGTTATTTCATCTAATTTGATGTCATATTTGTTTAAGAATTTAGTTTCAACAAATACTTTATTTTCAATAAATTTAATGATGTTTTCGTCTTTATTTGGTACATCTTTATATTTGTTATAAACAGTATCATATTCACTTTGAACAAAACCTATGGTATTGATTTTTTGAACTAAATCATCAATTTCATAAATTACTTTTTGTAATGAAGCTTTATATTGAACTAAGCTATCACCAATTGATTTAGTAAGATCATTTGATAATGATGAATAGCCTTTTAGTTTTGCTAAGTCTGCATTAATTTCTGATTTAAGGTTTTTTAAATTAATAATTGCAGTAGTTTTTTGTGAATTTTTCAATTCTTGACGAGCAGTATTTAATGCATCATTTAAATGATTAGTAGCATCTTCAATTTTAGTTTTTGATTTGTGATCAATATTGTTTTCAATAATTCGATCATAATTTTGTTTAGCAGTATTTAGTGCAGTTAAAGCCGTTTGCTCTTCACTAGTTATTGTGCTTGTTGTTTTTTTATCATTTAAGAATTGAATAATTCCATTAGCTTCATCTAGTTTTTGAAGATTTTCATTTTTAGCTTATTCTCATGCTCTTTCATATTCATTAGTTTTTGTGATTGCGTTATCTTTAAATTGGGTTGCTCTTGCAATTAATTGATCTAATTTTTCTATAGTTTTATCACTAGAATTATCGATTTTTACTAAGTATGCAAGGTTACTATTATTGTTCATTATTGTATCGATTTTTGTTTTAATTATTTTAATAATTTCATCATTTGATTGTTTAATTCCATGCAAAATAGAACCTTCATATTTACCGTCAGCAACTTTAGGACTTAAGAATCCATCAGTTTCATTTGCAATAGTTTTAAGTCGGTTAATTCTATTGTTGAATTCAGTATTAATTTTTGTAAGAATTCTATTGCCAAGAGCTTTAATAGCTACAAGTTTTTCATTTGCATCTTTAATTGTTGTAATTAAAGTTTTTAAGTCATTAACACCATTATTAGCTTTTTGTTTAACTGGTGCAATTGTTTTTTTATAATCATCTTTGTCAGCTAAAAAGACACTTGAATTATTTGAAATAATGTCATCTATTTTTTGATATTTAGCATCTTTTACTTCATTAATAATTTCCTGTAAAGCTACATTAAGATCTGCTTCATATTGATTTATTTCATTTATTACTTTTGTATATTGACCACTAGCAGATTTTTCAGCTTTTAAGTATTTTAGTGTCATTGAACTAAAGTGATTTTCTAAATTATTATTTGCATCATTTATTAAAGCTTTAGTTAAAGGCAATTTACTATCAGCATCTGAATAATTAGCATATGAATTAATTTTGTCAAAGTTTAATCTTAATTCTGCAAGAATGTCATTTCTTTCATTTTGTGTAGCGGCTAATCTATTTTTTAAATCATTAAGTTTACCTTCAATTTCTTCATAATCTCTTTTTACTTCTGAAAGTTTGATTACTTTATTTAAATTATTTGGATATTTATTTTTTAAAATAGCTAAAGAGTTTAAAAAGTCTAAATGTAAACTTGTTGTAATGTCTTCATATAAATCGCTGTAAATAGAATTATTAATTGCTTCTAGTTGATCTTCATATTCTTTAATAGTTGCATTAATTAAGTTACATTTTTTATTGACAACTTCATTAATTAATATTTCAATTAAGTCATTACTATTTTTAATTTCAATTAAAGCATTTTTAAGAGTTTTAAGATCATTGTTATTTTGATCAATTAATTCTTGATATTCTGCTTTCTTTTGATTATTTAATTTAGTAATCGAATCTGAATAGTTATCAACTTTATTAATACCAGTTAATTCATCATTAGCGGTAGTCAATTCTTGAGCTTTATTATTTGATTTTTGAATTTGTTCTTTTAATTTATTACGATAAGCAGCTGCAGCTTCATTTTTGTAATTATCTAATGATTGTTTGTAACCATCTAGAATATCTTTAATAGTTTGTAAATCATTAATGTTTTTGTTTTCTCATTCTTGATTTTTAATTTTGCTATATGTATTTTCATAATATTCTTGAATGGTTTTTATTGAGCTATGAGTTGCTAAAATGTGTTCATTAAATTCATTTGGAAGTGTATCAGCTTGTACTTTTAAATCATTCATTAATTCACTAATTTGATTTCTTAAATTAGTATTTTCTTGTTTAAATTCTGCTTTTTTGTTTTGAATTTCTTTTAATTTTGCTTCTGCTTTAGTTATATTATTTGTTAAGTTATCAACACCTACATCTTTGATTTAATCTTTATTATTAGCAGCGTTTTTAACTTCATTAAATAAGGCTTGAGAATTATCAAAAAGTTGTTGAATTTCTCGAACTTGAGCAGCAGTCATTCATGTTTCATTTTTTGCTTTTTCTTCTTCAACTTTTTGATTTGTATCTTGAGCTTTATTATTAACAGTTGCTACTGCTGTATTAATTAAATCGGTTAACTCTTTAATTAATGCTTCACTTTGAGTTTTTGTTTGATTTAAAATAGCAATATCAGTTGAGTCTTGATTGTCTTCTACAGCTTTTAAATTGGTTACAAAATTAGCTGGAAGAGTTAAATTGTTTGGATTGTTTCTAATGTATTGTACTAATTCTTTTATTGCTGAAATATTTTCTTTAATTTTGTTGTTAAGAGCTGTTTTATAGTTGTTTACAACATTTTCTAAATCTTGAATTGCAACTTTAGAATCATTAATTTTAGCTGTTATATTACTATTATTTTGATCTTTAGAAACTTCTTTTAATAATTCATTTTTATTATTTGCTGCTTCAAGTTTAGGTTTCACTTTTGTGTTATAATCCGCTATTGTAGCAGGTGTAATATTTAAATCATTTAGTTGTTTTTGAAGCTCAGCAAATTTGTCTTTATTTGTATTATATTGATCATTTGCTTGTCGAATGAGAGCTTCACGATCAGTTTTAATTTTAGTTAAATTACCAATTAATTTTTCTAATTCAACTTTAGTATTAACAATTGATTTTTGGCTTTCTTTAATTAAGTTAGCTCGTGCTAATTTTGTAATTCTATCAGCATCTTCATCAGCAGTTGAATTAGCTTCAAGATTTGTAATTTGAGCTCCATAATAATCTTTTGAAGATAATGATTTTAATGAATCTTTTAAATCGTTATATTCATTTAATTTATCTTTTAATGTATTTTGTTTTGCAACTATGTCTTCTGTTCTTTTTTCTTCAATAGCATCAATTAATTTTTGACCAAATTGAACATTGTGATTAATTGCATTTTCATCATCGCCTTTAACAATATTTTTCATTGAATCTAATATAGATTTTAAATCTTTTTTGGAAGCAGCAGTTGAACTATTTTCATTGTAATCAATTTTCTCATTTTGCATTAAATTTGAGTAATATGAATTATCTAAATTGTCTAAAGAATTTCTTGAATTTAAATGATTTTCAACCTTTTCAATTGATGATTGTAATTTATTTTTAGCAGCTGCTAAACTATTGTTTTTATTCAATACTTCTTGATATTTTGCTTCAAGATCTTTATCTAATTGTTGAAGTTTTGTTAAATTAACTTCTTCTAATTTTGAAGTTTCAATAGCTTCTTTTGTTGAATTAAGAAGTGCTTGAAGTTGATCGATATTTGTTGCTGAAGGATTGTTAGTTATATATTCTTCTACTGTTTTAATTGTATTATCTAAATTAGTTTTAGCTTGATTCCATTTTGTTTCATAATAATTAACTAATTGTTTAATTTCTTCTATTTTAGCTTTAATTTTAGTTATATGTTCTTTAGATGTTTTAAGATTTTTTTCTACACTTAATTTTCCAGATTCTTTGTATTCTTTTGCTTTTTGATAAACTGAATTAGCTTCAAAAGCAGGATTTAAAACTAAGTCATTGTCTTGATATTTGTCTTTACCTAATTTGACGATTGTTTCTTTTTTATAGTCTTTTTTGATTGCTTCTAATTCTTCAAAAGCACTATCGAATTTCTTTATAAATGCGTTTCATTCTGGAATTTTAGTGCTTGTATATTTTCCATATACATCATTGTTTTCTTTTACTAAATCTTCTAATTCTTTAATAGTAATAGTTGTACTATTGTCAATAGCATTTTTAATTTTTTCCAAACTTTCATTAATTAGTTTAATTGTTTTTGGAAAGACATCTTGATGTTCTGGACCATTGATGCTGTTTGAATAAATTTGTCAATTTTTTCTAGCTTCATCAAGTTTAGACAATAGGAATGTTCTATATTGATCTTGTGAATTTTTGATAACTTCTTTTAATTCTATAGTTGTATTTTTTACAAGACTTAGCATTGCTCGATCTTTATTTAAAGTTTTAACAGCTTTAATGCCTTCTTCAAGTGTTTTGAAGAAATCATCAGTATTGTTATAGTGTTCATCTGTATAATGTTTGATTAAATTTTCTGCTTCTTTTAAAGCCTTTTCATATTCTTTTAATGCTTGAATTCTTCTATTTTTAGCATCTAGAATATTATCATTTACAAATTTAACTCCATTAGTTAATTGTGCATTATAAATAGCACGCATTTTTTCATAGAAAATACGATAAATTTCTTCTTGTTTCGCTTCAATACCTTTAATATGTTCTGAACGAATTTGAGATATTAAATTGTCCATAATAATATCTAAAGCCATTTTGTAATGGTCATCTAATTTAAAGTAATTTTCATAATCAGCATTAACTTGCATTGGAATTGTAAAGTTATTTTTTAAATCGCCATCTAATAATGAATTTCAGTAGCTATTAGTTGCTAAGTTTGAGTGAGCTGCAACTTTTTGATTCATTTCATTATCTTTTGTATATAAAGTTTTTAATAATTCAAAAGCAGTTTTAACATCAATAGTTGAGTTTGTAAGTTTCGCTTTATTATCATCTATATATTGTTTGTAGATTTCTGAATCTTTGAATAAATTGTAAACATTTGTCACAATTGTTTTGTAGTTATTACGTGAAGTATTGATTAAAGCTTTTTGCTTTTGAACATAATTATCAAAATCAATAAGTGCACTATTAACAATTTGAGTTCCAATTGAGCTTAAACGAGTTGCTTGTTCTGCTAAAAGTGAGTCAACTTTAGTTTTGATTTCACTATATTTATCTTTTGAATTTGAATATTGATCTAATCCTGCATCTAGAGTTGCGATAAGTTCATTAGCTTTATTTATATTTTCATTAAAAATTGCTTCATATTTATCAAAATCTTTTTCTAAAGAATTATATTTTTGAACAAAACCTGGTGTAGTTAAATAAGCTTTAAATAAATCTATTTGTTTATTAACTAAAGCTTTGCTTTCATTTGTTGTTTTAATTTTTTTAATTAAATATTCGATAAATTGTGACAATGAATCTTTGTCATAAGTTGAGTCTATTACATCTTGTTTTATTTTGTGTAATTTTGCTTCTAAGGCTGTTTTATAGGCTAGTAAGGAAGCTAGAAGTTTATTATTAGCTTCAATTATTGCTTTTGGTGTAATAGCATCAATTGTTTGAGTATTTTGAACTAAAACTTCTAAGTTTTCAATTTCACTTAGAATATTATCTTCATTAAGTGTAGCATCAAAAGTAGCTTTACGAGCTTTAATATCATCGATCAAATATTTGTAATCGCTTGATTCTAACAATTTAGTTTTTGAATCACTTTGTGTGTCAGTTAAGATAGTTTTTAAAGCATTAATAAACTCTTCTTTTTTAGTTTTTAAGTTAGAAATTTCATCATTAATTTCTTTAATATTTGTTGAAGCAAGTTTAATTTTTGCTTGATCAATAGCTTTGTTAAATTCAATTATTGGATTGTTATAATTTGTTTCATCTTTAGCTTTATAGTTATCTCTTGTAACTTCAGCATCATTAATTGTTGCTTTTAAATCTACAGTATTTTTATCTAATAAGTCTTTTTCATAATTAACTTTTAATGTATCAACATGATCTGCTGCTTTAATTGTTGCATCATTTAAAATTTTGTCGATTTCATTAATATTTTTAGTAATTGAAACTAAAACATTTTCATATTCAGCAACAATAGCAACTGTAACTTTCGATTTAGCTACTTCAGCTTCTTTTTTAAGAGTTTCGTATTTTTCTTTTAAATCTCTTAAGTTATTTCTTGCTTGAATTTCTGAACTTGATGTTCCTTGTTTTACTAAATTACTTAAGTAAAATTCAAAAGTTTTTTCATTATTTTTATATTCTTTATTTGCTTTTTTAACTATATTATTTTCGAGATTATCATTTGTAATATGACCATTTAAAACGAAAACAACTCTAACAGCATCTTGACCATTAGATTGAATGAAAGAATAACCTTTAAATGAAGCTTTTAATGAACTTGGTATTTCGTTGTAAATAAAATGTTTATTTTTAGAAATATATTTTTTAAGATTTTCAGAATCATTTTTAGGCATATTATCTAAATATTCTTTTAATTTAGATTTATCTTCAAATAGCTCTTTTTCTTTTAAAATAGTACTTTTTTCATCAAACTTCTTGAAACCACTTATTGGAACAATTTTTGACTTTTTAACTTTTGTATTTTTATTTGTAATTTCAATTTTGTAGTATGCAGTTGATTTTAGAACATTTGATCCTTCTAGAGAAACATTAATTTCATAATTAGAATCTTTTTTGTATTCAACATTTGTAATTTTTGCATCACTAGCATAAATTTCACTTACATTTTGTCCTTTAATTTCTAAATTTTTTAGAAAATCATCATCAGACATTTTTTTAGTATTTTGTGTTTTTATTGCAATAATAGTTAATAGAATTGAGCCAGTTATAGTAGCTGTTGCAATAGCTCATCCTAATCCTTTTGTTGCTTTTGAAATAGTTTTTGTAGACATATTTCCTCCTTAAAACAATACTTTTCTATATTTTTTTACTAAAAAATTATATATTAAAATATATAAAAATTAATATTTTGAATCAATAAAACAAATAAAAAAATATATTAACTAGTTTAATAAATTATAACTTTTGAAAACTTATAGGCTAAATTATATTTTCAAAAGTTAAATCGAAGGATGCTAAATTATAGTTTCAACCTTTTTTATTGTTATAGTTAAATTGAAATGAATGTTAAAAATATATTTGTTAGTTTCTTCCTTTTAAAGATATTTTGGTAATTTCAACATAAGCTTTTTCAAGCTTAATTTTATTAAATCATCATTAGAAAGTAAAGAATCATTTAATTCATTTTTAACACTATTGCTAAAATATTTAAGATTGAAATTAACAATATTTTCTGCTCTCAAATTATTATTTTCTTGATATAAAAATATTTGTGTTTTTTTACTATTAAATACGGTTTTTAAAATATTTCGTTCAACACATTCGTAGTTAGTAGCGCCTTTATTTTTTAAATCTTGTAATACATATTTAAATTTTTTGGCACTTTCTGCTAGGCCCAAAAATTTATCATTAGCAACTATTGAAAACATATCATTTTAGTAGCATTAATTTGAATGAATCCATCTATTTCAAAGTGGTTTTCGTCAGTTCTTACCTTTTCATAAATACCATCATCAAAATATAAATAATAACTTAAAAATGCACTACTTTTTTATCATCATAACTATATTTAGTTTTAAAAATAACCTTTTTGTCATTATTTTTGAATAAGTCATATTTAGGCAATTTGCTTGTATATAAATATCTACTTGTATAAGCTTCATTTGTATCTTCAAAACCTTGTCTAAATCTTGAAGCAAAATAATTTTTATCTTTAAAAATATTTTCTCTCGAGTCTACAGAAACAAGTTTTACATATTTGAAATAAGCTTGTAAAAATTCTTTTTAGAAAATAAATTTTCTATATCTTTTAAATTTAATTCTTTACTTAAAGCTAAAATCATTAGGTTTTTAACTTTTTATATTTATTGTCATTTAAATAAACTCTTATTTTTAAATTTTTAGTTCTTTATTAATTAAATTTAAATTAAGATTAATTTTGCTCTGAAATAAGTTTAAATTATCAACATATTGATCTAAAGTTATAACGTTATTGGCCGATAATTTTGAAGAATCATTAACTCCAAAAAATAATTCTTCAAAAGATTTTGTTAATTTAACTATTTTGAAATTATTAATTTTTACTTGTTTAATTTTTGATTTAACTTTAGCATTGCTTTTCAATTGTAAGTAAAAATTAAATGTTACTTCACTATTATTTTTTATAATTGAATTTATTTCTAATTTAACTTCATATTTAGCCGGATCTAATTTTTTTATTCAATTTATTTAAATCTTCAACTAATTTTTTAGCTAACTTTTTGATAGGTTTAACTGATTCAGGCTTCTTTTCTTATTCTTCACAAGATGTGCTCATTGCAATAACCTTAAAGGTAAAAAAGCACTTAAATGCCTTAAAAACTTATTTTTTTCATAATAAAATTTTTCTTCCAATGTTTAATTGTGGTATTCATTATAAATGAAGATTTAACAAAAGCGAATAAATTATTTTAAAAAAATAAGAGAATAAATTGGTATGAACCCCAAAAATTGGACTTTTTAATTATGTTTGGACTTATTCCTGTATTGGACAGGACTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTCCTAAAGCAGTTTTAAATTCTTTAAAACTATTAAATTTCTTTTCTTCTCCAAATCAAAATTCTCTTTTTATAACACTAAATAAACATTCAGTAGGACTATTGTCTAAACAATTTCCCTTTCTTGACATGCTTTGAGTTGTTTGTTTTTCTTTCAAATAATCAATATAATCTTGATGAGTGTATTGTCATCCTTGATCAGAATGTAATAATACATTTTTTAAGCTGTGGCCATTCTCTTCTACGTTTTCTAACATTTTACCAACCATTCTTAAATTAGGCGATTTAGAAATCGAATAACCTAAAATCTCTCTGTTTTCAAAATCAACTACAGGAGAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTGTTCCTAGTATTTTCAAATATTTATTATTTGAAAAATTTCTTTTGTATCTGAAGAAATCTTCTGTGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTGTGAGCATCACCTTTGAATGAAGAATATTTATTTTTTCTTCTTAGCGTTTTGCCGAAAATATTCATTGCTTTCATTAATTTTTGAACTTTTTTATGATTGATTATTATTCCTTTATTTCTTAAAGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAAAATAACCTTTTTTAAATTTTCATCTTTATCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAGTTGACTTTTTCAATTTAATTTTTTCTAATATTAATTTAATAAAAAATAGCTTTTTAAATTCTTGCCTTAGTTCAATTGTTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAGGCGCTCACTTTTTTTGCTATCGCATTCTCCAATTTGAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATTTTCTATTTTATTTTCAATATTGATTTTAAAATTTGTTGCCTTTTGAGCATCTAAATTTATTTTAGATTTTCTTCCTTTTTTCTTTTCCATAGCAAAAGAATTATAACCAAACTTTTTGTATTTTTCTATTACTGAATAGCATGCTGAATAAGAGTTTTCCATTATTTTAGATATTTCTAAAATACTAAATTCTTGAAAATATAATTCGATAATTTTTATCTTTTGATCAAGTTTTAATTTTAAATAACCTTTTATATCTCTATTTCATTTGTTTCTTTTTACTTTTTTAAATTTAAATTGCATAAAAATGAACCCCTTTCCAAAAAGGGAACTAATATAAAAAGGTGTGTTATAAGATTCTCTACGTTTTCTTATTTCGTAATTATTTTATCATAATCCATTTTGTAGCAAAGGAAAATACTGCATGTGAAGAGCATGCAGTTTCCTTTTGCGCGCACACGCGCTACTTGAATAGTACAAGATAAAGACACTACAAAGCTATTTACCATAGCGTGTTTTATCTTGAAATGATTTATTCATAGAATAAATCAAAAATTAATATTTTCATATTTTCTAGGCGCATGCACTTTTTTCGTTTTTGCCTAAATTCGCATGCACTTTTACATGCACTTTAACTTGTATATTTTTATTTTAAAAATATATTAATACTGCTTTTGCGCATGCACTTTGTTAAAAATAATGCTTAAATGAAAAATAAGTTTGCTTTTGTTTCCATATTTTTTGCAATGCTTAACTGTATTGTTTTTAGTGAAAATGTAGCAAATTTCTTTGAGCAAAACCTCTTTAAATCGTGCCCAACTACCGTTTCAGCACAGCCTCCAAAGTAGTTTTTAACTAGCACTGAGTTTCAAATATATTCTCTATTTTCTTTATAATATTTCTTGAACTTTTTAGCATTAGCATATACAGTTTTATTTTTCTCTTGCTTTGTTATTCACTCTAAAACACTGTCAAGTTCTTTCTCAAATTCATAAATTAATCCTTTAGAAAACAAGTTGTCTAACACGCGATAAACATCTTTATAATCCCTAAAAAATCTCTTATTACTTACATTAATTTTTTTGCTATATCCAAAAGCTTTAAACATATTTGCTTTGAAATGAAATGGATCATAAACTCTCGTTGATTTTAAATTATCAGCTATTGTAGTTATGAATTTTGCACCATCTCCAGAGACAATAATTTCACAATTTTCTTTATATAAACTGTTTAAACTTTCTCTAATTTTGTTTTCAAAATCTTCTTCACTATTGAAGTTTTTAAACTCACTTGCAAGTGGTAAAAACTGCATAATAGAGGTCTTATTTTCTATGGCTTTTACACTACTTCCGCTAAGCAAAGTATGTATAGTCGCCATACGCACTCTAACCTTTATTTTTCTACCTTTAAAAGACACTCTAATATAAGCATCATCAATATCTATTTGTATAGTCTTGTACTCTAGATTTTTATAGTCTTTTAAAGTAAAATTATTTAATACTTCTTTCTTTAATTGTTCCCTATGGTAATAATAAACGTGATAAGGAATAAGTAGCTTACTATTATCACCTTTTGAAGCGTATATATTGTTTTCAACACATTTTTCGACGCACTCTTTATAATATTTATTTCTACCGTTATCAGCTAATCCTTCACTCGGATAAATAACTAGTCTTTTGCCTGTAGTTTTATCTTTATACATGTAGTAAATAATTCTTACTGTCTCGCCCCAAATAAAAATATCTCTTTCTATTTTTCTTTCAACATTATATTTAATTTTACGCTCTTCGCTATTCTTAAAAACTTCTGCTTGAGCATTTCAATTGTCAGCTAAAGTTTTACTCATTTTGTTCTTCACAGCGCCCTCCATAATCAATTAAAAACGACGTTTTCACGCCGTTAATAATTTTATATGTTTTACTTCAGCTTCGTTTTGTCTTTGTCTGTTTTGCTTTCTTTTATTGGTATTTCCTTAGCTACTTTATCCTTAGCTTTTTCGATATTTTTATTATCGTATGGATCAATATAGCTTGATTCACTTTTGCTACTTTTAGATAAAAAACCTATATTTTCAAAAGAATATATATTAACGTTATCCTTCGTCTTATCTAAGTCTTTTCAATCATTATGAGCAATTTCTCTAATAAAATAATTACTAGTTTCTTTATCTAATTCATACTCAAAATCATTTTGATTAATTATATTTTTCTCTTTACCAATACATAATTTAATATTTTTTAAAGCTTCTTTTGTATATTTTGTATTAGAAGTGTTTGTGTTAATTAGGAATTGGGATTTTGAAAAATTATTAGCAAAATAGTTTTTTGTTGCACGCTTAACTTTATTACTATTATTAGACAATGTAGATAAAAGCTGATTATTTATTAACTCATCGTTAGTATATAAAACTAAATTGTGCTTTAAATTATTTGATATCTTTATTATATTTAAATCGGGAGGAATTGCATTTATACGGCGACTTAGGTCATCGACTATCAATGATTTATCATTTTTATTTAATACAGAAGTATCTTGATAAATTGCTTTATCTATATTACTTTCAAGCAATTCCTTATCAATAATAAATATATCCTTAGTAACTACTACACTTTCATGATTCTTTCAATATTCGTTGGTTTGATAAGAGCTGTTATCAATTATTAAATTATATTTGCTAGAACTTTTTCTTGCCGTTTGAAGTCTTATACAATAATAAGTTTTATTTTTTGTATTTTCAAAAATTATCAAAGGATGAATTTTAAATTTTGTATCGATATTTAGATGAAAAATATCTTCTAATAAAGGATTTGTAATTTTTAATGGTCTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAGAGGATTAAACCTCTTTTTAAGACGAACTAGAGTCTAAGGCGTATCATCAAGCGCTTAAAGATAAAATATGAGCTGCTTGCTTGTTCGATTACTCATATAACAAAATCCTTCTTAGACTATTGTTTAAATTATACTACAAATAAATAAAAAAATTAATAATTTTTCAAATCTTATTTGCATTGTTTACTCTTAAACAAATTGATAATCTTTTACTAAAAAATTATAAAAGGGTGGGCTTGAATTTTTCCGTTATTTAAAACTTTGCCATCAATTGCGGTATATACCATATTTAAATAATCTTCAAATACTTGCACTTGTACTGGAGTTCTTAAATTGTCAAATTTTCATTCGTTAGCTTTGTAAGACTTAACACATAGCTTAATGTTTTTTAATCTTATTTTCATTTCTCTTACTATGAAATATAATAGGAAATTTGATTTAAATAATTCCATCATATTGCTAATTCAAGTAAGATAGTATTTTATTTTTCTTCTTATTTCTTTATATCAAAACTCTATAAATTGTATATGAGTCATTAAATGACTGCAGCCATGTTTAGAAGAATTGACACAACTCATTAATTTGTTAATATCATAACTTAATGGTAAGTCTGACATATCACTAACCAAAGCTCACAAATCATTTAAATCTTTGTCACTACAATTTTTTAATGTTTCTCTTGAAATTAACATTTTTATTTTCCTTTCTTTATTTTTTAATATATAATAAATTTGCGGTTGTTCGGTTCAGGTTTAAGAGCAATCGCTCTTTTTTATTTAATTAAACTGAACAACAATGATAAGTAAAACAAAAGGTGAATGGCAAAATCCACTCACCTTTCCTTATTATTAAATATGAAATAATAAATCTAGTGATTAGCAAAAGGTTCAGGATTTTTACTTATCACAATTATTATATCGGGAATATATAACAATGTCAAATAAATTTATGAAAATTTTTCACGTTATAATTTAACTATGAAAATGCCAAGCTATAAAGATCTTAATTTTAATATCAAGAGAGAATTAAATAAATTAAAACCTAAAAGCTTTGAAGAATGAAAAGAAGCTAAATCAAAAGTTTTTTGCATTGCTCTTGGTAAATATTTAACCAAAATTAATAGCCACCAACCTGATATATTTTTTATTTTGCAAATAATTTATTAATTTCTTCATCGCTAAGAACAACCGGCTTAGCCTTAATTCTGTCTTTTTTATAGATTTGTATTTCTTTGAATTTCTTAATTGCAACTTGTTTCTTGCTCTTGCCAGCTTTAAAGCCTTTTTTATTCAAGTATCATCTAATACCATATATGCCACCAACAGCAAGACCTAAGGCAAGCATAACCGATACAATTCAAATTCAATAATTACTTTTATGGCTTGCTTTTGTTTTATCAACATCTAAATCTTCACCATTTGGCTTTTTATGCTTATCGTTTTGTATTTCTTTAAATCATAATTTATCATCAAAAGCATTATAATTTGCCTCAATATTATTCACCACTATAGCATAGGCAAAATTAATATTATTGCCTTCTTTAACTGGTAATAAAACAAAATACTTGCCTCTAGCTTTATAATCTAAGCTTTTATTAAAGTTATCGTTTTGTATTTCTTTAATTGCCTTTTCTAAAGCTTCGTGGTACTTCTTAAATAGCTCAGTATCATTTATTTGATACGGAATAGGAACTTTATGTCCTTCAATTTCTCAAGTATTATATTCAGCCATTACCTTTGCTTTAGTTTCTTCATAAACTTTTTTGAATTTTTCATATTTAGCTTCATTTAATTTATCAATATCAGCTACTAAACCAAGTAAATAGAATGAAGCATTTACAAGTGAAACTAAATAATCTTTTTTAAACTCGATACCTGAAGCAATCGTTATCTCATTTAACTTATTTATAATATCTTTGGTAATTTTCTCAGGTTCTTTTTGCGATATAACAATAGGATTTTTTACTAGTGTATCATTAAGTAGTGATAAATCAAATGCTGGTGCTTCAGTATCATTAATAATATGAATCTTAGTGCTTGAAATTAAATTAGCATTAATACCTTTTATCTTAATATCAATACCATCTTTAACTTATTCCCCTGTACTAGTAAATCTAAAACGTTATCTAGTCCTTCTACTTTAATATCTTTATTCAATAACATTTCTATGCCAGTTTTGTTTTTGTATAAGTTGAATTGTTCTTCTATTAGCCCTATCAACTTATTTCTAATCTTTTCTTTTAATTCTTTTGAATCATTACTAATATTCTTTCCCATTTCAATTTCGAAGTGTTTTTGAATGTCTAGTGATGATAAATCAATAGGAGGATAGTACACGTGGTACGCACTATTTTTCAAATTAAATGTTATGCTGTGATTTATGTATTTACCCGTTCCACGCAAAGTAAAAGCTCATCCCTTACCGTCATTATCTGGTACATTAATATCTTTAATATAAACTTTTAAAGCCCTATCCAATCAACGCTTTTCGCTTTCTGGCGTATTTCAATTAGCTATTACCCAGCCACCATTAATTTCATTTTCAGTTATTCCAATTGCCTTTAATTTAGCACTATTAGCAATATTTCTTTTAACAAAATTAAGCACGATTTCTTTAACTTGTCTTCTATAATTTTCTTTAATAGCTTCATTAGTTTCATCTCCATAATTCAAGCCATTAAGATTTTCTAATTTACCTCAAGTTATTTTATCTTTAAAAATATTTTCATACTCGTTTCCTGTTTCTACATTAAACTTTGACGCTTGGTCTACAAAGTTTTTATATAGTGAAATCATCTTGTCATATCCTAATTTACCCAAGTCATCATTTTGGATACCACAAGCTTCATTTAAGTAATTTTGAAATAGTGTAGTTATTCAATTGCCACCATCTAAAAATGCTTCAAAATAATCTTCACTTTGATTATTAATTTTTTCTATCTTGTTTTCAATTGAATCCAAGAAAAAACTTTTTGGCTCGCTCTTTGCATCAATTAATACCAAATTAACATTTGATATTGATTTTTCATTTACAGTAAATAATAGCCAAATGCCTTCCTCAGACATATAACTATATTCACTTGTCCCATTATTTACCGGTATTTCAATTAGTTTTTGTTCTTTCCTACCAATCTCAACGTATTTGTCATTTTCTTTTCTAAACAACAAACACTTATAGTATCTTGAAGATGACTCTAAAACTTTATCAACCACCAAGTTTCTTAATGCCCCCTTACCAAGCACATTAGCTTCAGCATAGATTCCAAGATCTGCTTTATCTGTAGTTATTTTAATATTAGGATATACAAAACGAAGTTGTTCTTTTAATTCATCACTAAACAACTTATTTTTAATTCAAACAATCTGCTTTTTAGTTCCTGTTTCTTTATCAATACTTGGATCATAATTCTTATTTACTATTAATTTGCCTTCTTTATCCTTTAACTCTTTTCCCTTTGAATCAAGCATATATGGAGAAATAATTTGTTTTTGATCCATATTCTTTTCCGGCTCTCAAGCATAATATTTTATGTTTTGTTTAAACTGATTTCCAATTACTTCATAAATAACTACATACTTTTCTTCCGGCTCTCTACTATTATTTGAGCCTTTCTTGTATCTATAAACTTCAACAAGATATTCATTTTTTGCAACATCGCCTTCGCCATTACCGCTTCCCGAGTTTTTATCAAAAGGTATTTTAGATGCATCATTAACATTAACTCTTTCATCAATTAATTTCTTTTCAAATATTCTATTTAAAACACTAACCTTTTGCTTATTTACCATTAAAACTTCAGTTTCATCCTCTGAAGCTTTAAAAGAAATATTAATTGGCCCTCTAACTACATACCTTCCACCAAATCATGGCTTACCGACAAAATCTTTTTCAAGTTCTTTATTGCTTACCCTTTCAGGTTCGTCTCTTTTAATTAGATTTTTTAAATCGCCACCATTAGCTTTTGTCTTTGCTTCTATTTGACTTATACTTCCATCAGGATTAGTAATAGAAGTTGATATAGTTAACCGTGAAGCTAACGAAGCTCCACTAAGTCTTTTATACAAATCTGATTCTAAAATATTTACCTTTAATGAATCAAAAACTTTAAAATTACCTAAAACGATATCTTTTAAAACCCTTAAAAATTGATTCCACTTCCCAACAGCTTCAGCTTTAATGTTTTCTTTTGTTCAGTCTTCAATATTATTAAGAATCTTATCCAAGTCTTTATCGATAATTTCCTTATCATCAATCTTGACTGATAAAACCTTAATATAAACATCAATTTTGTTCTTTTCACCAACAACTTCGTTTTTTATCTTGTAATCATATTTGTATTTAACTTTAATTCCACTAGCATACTCAAACTCATATTCTTTATCGAATATTTTGTTTAAATGTTCTATATTCGATTTGCCTCCATTGTCTTTAAAAACTCCATCAACTGATGTAGTCAAATCGCCCAAGGTATCGCTTAATAAATCTATAGAGTAGTGATTTTGGTCAATTTTCTTATTTACCCCATATTCAAATCTATTCAAACAATTATCACTTGAAACACACTCTTTTAAAGCCTCTATGAATCAGCCAACTTTAGCATATTCTAGATTATTTAATACTTTATTACTATAGTACCTTACATCATACATTCCTTCAATTTCACAATCAAAATCATGAAGCCCCCACAAAAACATTTTATTTTTATCATCAGTTAGCCCACTATACACCCTTTTATCTTTATCAGTTTCAAGATTATTTAAAAACTCTAATGTTTTTTGAGAAACTTTATATTCAGCAAACAGGTTAGTAAAACTAGCACCTTTATGTTCTTCCATAAAGTTGTTTTTGTCTCATTTTATTAATTGTTCTATAAAATTAGAAGCAAAAATTACTCAATTCTCTTTTTCACGAGGATGCAAATAATTTAGATAATATTTATCATCGTATCAAAACCAGCCATAACTATTATGATTACTAGGATTTGTGTAATAGGCTTTTTCATAACGCACTTCAAAAGAGCCTAAAGTTGGCCTAGAAACTATTGACGTAATTTTATTTTTTACTGGGCCAGTTTTATGATAACCAACCCATTGGTCCTGTGCATACAATAATCCTTTATCACATAGTTGTGACCATTTTACTTGATACCTATTTTGTAGTCTTTCATTTTTAGCAATTTCATCATCTTCCATACTAGTAAAATTGGAAAATAAACTATTAGTATCAATCTTATGTTTTTCTTCAGGAGCTTTAAAATCTTTTCCATAACCTAGAAGTTTTTTATTAGTATTTAACGATAAATTAAGTACTGACGGAATCGCAATACTCAATACACTCAATGGAATTAATTTTAACATCTTTTTCTTATTCATTTTTTCCTCATTCTTCGCCCGTGCCTCCGCGTGCATATTAGTTATGCCCGTGTGCGCACGTGTGAATGTTTAAAGACTATTTTATTTTTTAAAAAACTCACTTAAATCAAAATCACCAAAATCTTCATCCTCTTTAGCATTATTTAATTCTGAAGCTTCAAGTTTTGCATTTTCTTTTTCAAACACTTTTTCAATTTCATTGTCTTCGAATTGTTCTGCAAACTTGCTTAATGAATTGTCTTCATTTCTTACCTTTTTAATTACCTTCTTATACCATGCAGAAGCCGATTCATTCATTCTTTCACGCAAACGCTTTGCATATTCTGGCTCTTTAAGCAAATTATTGTTAGGTTCATCTAACATTCCTTTATCGCTTATAAGAGCATTTAACAACATATTGACTTTTAAATCAAGCAAAACCAAATCAATATTTTGCTTGTTAATTTCATTTATAATCTTGCGATAAAATGGAGTCAAAGACGCTCAAGATACTTTTCTTAATGCATAAAATAAATCATTTTTGAAATCTTTAGCTATTTCCTCTTTCTCTTGTTTAATTAAAGCGTTGCGAATTATATCTTTAAGCTCTACATACGGAGTAGAGCCACTTTGTTTAATTCTTTTTACCCATCTTTCGTATAACGCCACATAATCTGGTTCACTAAGACGCGTAGAAATGCATTTAGAGCCGTTTTTTGAGGCTCTAGAGCTTTTTACATATAATTTATCACCTTTTTTATTTTCTTGACTAATTTCTCATGTTTTCATCTTCCCCCTTTTCAAAGAATAATTCCTTTTCCTTTGGATTGTAATGTAGATTAATTTTGTATCTAAGTGCATTACTCATACTTGCTAAAACTTTCCCCACTCTTCTTCCTGAAATAAACTTAACTTCAGAATCTAGTAATGGAGCTTCAGCCGTTTTGTATAACTTTTTAACCGCCTCAATATCATCTCCTACTAACCCTAGAAAAAATGAATATTGACAGTTCCCCGTAATCTTTTTAGCAATTTGTTCAACTGCCGTGCTTGAAGCATAATCACTAGGATTTTGCGTGGTATGTATTGTCCCGCAATCAAGCTTACGCACAAGCTTTGTAGTATCTGATATATAGTTCAAAGTAGTTATATTCTTTTCACTAATATACAAGTGTTCTTCATCAATACAAAATGAACAATAACGTGTTAACTTTTGTATTTCTTTCTTGCTAATTAAACGAATCTTATTTTTTTCTTTATATTCGTTAATTCTTTTCTTGTTATTAAAAGCATTATCAAAGATAAAGCCATTCACCAAGCTAATTAAAACCATAATGCCAATTCTTGCATTTACGCTACCTTCAGTATCGATTAAATTACTTGTTTTAAAAACAACCAAATCGTTTTCAATGTTGATATTGGTGTGACCATTATATAACGCACCCAAAGTTCCATATCTTCCAAATTTACCCTCTAATAATTCAGCCATATCTAAAACCTTTTTAGTATAGCCTTCTTTATCGTGATGGTCTTTAGGAGCTACAAACTTTTTCATTTGCTTAATCAAATCATCCATAGTTGGTCATCCATCGTTTTTGAGTTCTTTAACTTCCTTTAATCTATAAATGCCCCAACTTTGATATAACTTAATAATTTCAAGTTGCAAAATGTTCTTCTTCATTTCATTCATATCATCAAAAATGAAACTAAAGAACTTTTCTAACCAAAGCAAATGATTATTAATTAAAGTATTGAAATTAACATTGGCATCATCTTCTTCACGCGCAATAAATGATTTGACTTGTAATGGATTAATAACCGTTCCATCTCCAGAACCTAAATCAACTATTTGGCCTCCCATTTTATTTGCCCATTTAACATATTCAGCTTGCGGATCAATAATAATTGCTATATTCCCCGCAGCCAGTGATGAAGTAAGAATCTTCTTGGTTAAAGTACTTTTGCCCATTCCTGAAGTTCCCAATATCATCACATTATGATTTGCTCTACTTGAAGTCAAGCTAAACATATTTCATACAATAGGACTATCATTTCCTCCCACTTCATTTCCAAGCAACAAGTTGTTTCCGTCATTAAGTGGCAGATTTTTCAATGGATAGCCTATTGCGTTATTTATACTTGTGATTTGATAGTACTCTCTATCTAATTTGCTTAATGGATAATTAGACATATCTAAAAATCCTTCAAATTGTCTAAAGGTCAAATCATTAATTCCAAAATTAGATCTTGTAAAACCATTCTTAATTTTCAAATTATTTTGCTTCAATTCTTCAGCATTTTCCGCTTCAGCTAAAACAAAAATATTTGAATCAAATAACTTAAAATTATCCGCTTGTATTTGGTCAATCATAACCGCAATCGATTCTTCCTCTAATGCTGAACCGAATCTATTAATAATTGAATTATCCAAATTACCAGCATCCATATTTTTCTTATTCGCTTTATCGATTATCTTATTTACAACATTTGGATCGTTATATGGAAAATTGTTTCACACAACTGTTCCCGATACATCGAATAACTTCTTTAATCACTCGTTATTCAAGCGTATTGGAATGTTCCCAGCACATTTAACGCTATACAACTTATCATCAATTTTTAATGAATTGCTTTTAAACTCCACCTTTTCATACTTGAACAAGCGGTCCAAACTATATTCACTTTCGTAAATATCACTTTCATTTAAAAACGTTTCAATATCTTCTTCGCTTTTATTAAAGTGATTAAGTTTATTCAAAAATGATAATAACTCAAAGCCACTTAGCTTTTCATAGCTAATAACTTCATAATCTTCAACCTTTTGAATAAATGTCGAATAACACTCATCCAATTCTTCTAATGTTGGAGCATTTAAAATTAAATAAAATGTTTCAATCAAATTGCTTTTAACTTCATTCTTAAAATCAAGTTCATTAGCTTTAAAATAACTATTTCAAAACTCTTTTGTTTCTCCACCGCTATTTAACATCTTTTTACTTTGAATCGTGTTTATATAATTTAAATTACGTCTGTAATCAATTTGTTCGTCTAATTTCACTATGCTAAATTTATATTTAAAACTATCCAATATTCTTGCAAAGTTTTCAATCAAAACCACTTTAGTATTTTCATTTTCAGCTCATATATTTTTCCCACCTAATTTAAAAACACTAAAGTAATTACTATGTTCACTCTTTTGAAACAACTTATTTAAAAATGACTTGTTTTTAACAACATCGTTTTCTACTAATGTTGAATATGGATTTAAATCAGAACTATCTCTTACATTCTTGTCATCCACCACATTAGAATACTTCTTTGGCATCGCCTTAAATTTAAACATTCTAATTAATAATTCATATATCTTGCAATTATTCTTATGTGTTGGTAATATCAAAATAAACAATAAAACCAAAAGTACACTAAAAACAATTCATTTATAAGTCTGCTTAACTGGATACAAAGCAACAAATGAAATAAATCAAGATATACAAGTTATTACAAAAAACTCGATAATATCTTTTCAACTAAATCTTCTTGTGGCTTTTGCCTTCTTATATTTTAATCGTTTAGGTTGTAGCACATTTCTCCCTTCTCATTATTCTTTAGGTTTAAGGAATAATCCTTTTCAGCCCATCTTCTTTACATTTTCAGCAAGCTGTGCTCCACCTTGAGCAGCTCTACCCGTTTTTGCAGCACTATTTCCCGCAGCTGAAGCCGATTTAATAGTGTTTTTACTACTTACACTAGTTCCAATAAATGAAGTTATAATTTCGCTTATTTCGCTGTATTTTAACGCTGTAGCACCATAAATAATTATTGAAGCTAAGGCTTGTAATACTCATCCAAAACCATTTGTTGATGATGCAATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCACCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAAAACAACCTATGCAAGCCATTTATAAAAGCAACAACAATATCATTCCCTTCGCCAATCATCAATGTAATTATTTTTTGTACAAATAGATTAAAAAGTAAAATAGTAATTTGAAATGCTAAAAGTATAAACAAACTTTTAACACTAAATACCAACGCACTTCTCATTAGTGATACACTCTTTTCGCTTTCACTAAAACTGAACTTTATAATCAGCACAAAAAACATCACCAGTCATATAACCGCCGAAACTATCGCAAATCTAACATACATATATGGGCTTTGATGGTCCAAAATGTTTTGTCTCGGCATTATGCCAAAAATAACAAAGTTTAATAAATCGAAAGTTGCTAGTTTAACAACCGATAATAGAATCGCTAATAATATAAAAACAATTAGCGATACAAAAAATAGCAATAAATACAATACATATGTTGGACCATATTTGAAAATCTCCATCACACGGGTAAAAAATCCACTCATTAGCTAGTCTTTCCCCCGCTTGCTTGAATACTAATAACTAGTTCAAATATTCCTCAACCCACTATAACTAGAATTAATACAATTCCAGCTCACAAAATCTTTTTATTGTTTTGTTTTCTTTTTTGTGGATCATCTTCATGTGAGTTTTGTATCCCCGCCACAATCGAGGTAATAATAACGCTAATAACAGCCACCACAGCCACAAGTCCAAATATGATATGTCCAACTTTCTGTACTTGCTCTGTTAAATTACTTAATCCTTCTGTATCAATAGGTTTCATTTTTAATTTCTCCTTTTTGTCTTAATAAATTAATACAAAAAAACTAATAGAAAAACATCTATTAGAAAAATAGTTCTTTGCACACATTATTATGTAGTAAAGTTAGCATAATTTTTAATCTAAAGTGCTTGTATCCAAGCTTCCATTAACTCTATTATTCCAAATTGCAATTCAGCTATGAAACTTACTTTTAGCTTCTTTTTTATATTCAGCCTCTTGCATTTTATTCATAAATTTAGTGTTTAATTCAAGTTCTTTAAAACTTTTATCTTTAGCTTTAAAAACAGTATTTGTAAAAAAGTTTATGTATTCGTAAATTGGAGCATATGGTGTATTGCCTGACTTTTGATTATCATATTCAGTAAAACTATATGGCACTTCTCAATCATTAGCCCCATACATAGGCATTAGCACATCATATAGTGTTCTATACGCCCACACGTAGCTATATTCATAATCAGCAAATTGACTATCACCAATAGCTACTGGAAACATCTTAACTAAGGTTTTTTTCTCTTTGCCTAATTCTTCCATTTCCACACCAAAGACTGCTCCATATTTAAGAACATTAAGCAATGCATTTGTTGCTTTATCCAAGTGCTCTTTAAAATAATTTCTATATATTGGTTCAATTTCAAACCACTTCTTATTTCCAGCTTGAATAGTTCCAAACATTTGCAATAACTCTAAATACTTTTTATAAGCTTCGTTATTGACTAACGTTAGCTTAACCATCTTACCAGCCATCCAAGCAAAACTACTTAAATCATTTCAAAGATTATAATCATCACCCGTAGGCAATTGCATTATGAAATCTTTGTTTTGTTTTGCAGTCTCATAAATCTTAAAGCTTTCATGGTATTTTTCAACATTACTTCTTGTAAAAGCTAAATAAAACCATTTTGAATAATCGCTTCCTAAGTCGAAATAATTATTCCATATTCCTTGTTTATATAAACCATCAATAGCAATATCTTTAAATGTCTTGCTTGTGCCTTTAAGAGCAAAATCATTGATGAATTGTTTATAGTAATTATCAAGGTCCATATCAAAATAATAGTGATAGTGTTCTCTTACTTCCTTTTGGTCCTCAAACACTCATTTATTACCATGATAACTATCCTTATAGGCAAATACATCTTTTTGCGGTCCTTCTAAATAACTAGCCATTGAATTAATATAATTCTTTGAATCCACAAGACCTAAATCTTTATATTCAGGATTCAAAAAGTTTCTCATCATATTATTCAACTTCACCGGCGCGCTATAATCAACATCGAATAAATCTTTAATTTCGTTTAATTCAGGTTGGCTAGTATACCAGTCTTTATCCAAGTCTAAACGAACATTGATTTTAGGCATATACTCGTTCTTTTTTCAATTATCATTTCTATACACATCGGCATCATGTTTTTGCTCATTATGCAATACTTGTGCAAAATAATATTGATTGTCCTTCTTTAGAAAATCTTTTGGATTCTTTTCTGCATCATAATGTACATAGCCATCCACATCGCTCCAATCCGTGCTTTCTCCGCCACTAGGGTTTGGATTAGGGTTCGGATTTGGATTTGGAGGTTGATGTGGCTCAAAAATAGGACCAAAACAACTCGTCGTTAAACTTGCTAAAGGAACTATCGTTCCAATTGCCATAAACTTCATAAACTTTTTACTTTTTTTCATAATAATACCTTTACATAATAATTTTAATGCAAATCACTATTCTTCTTACTTTGTTTTCAGTATTCTATTCGTTTTTTCACTCAATATACTGGATATATGAAAGTCGCCATTCCAAGAAATAAAATTATTCCTTCGATAACTCCATACTTAGTTTCTGTCAATTCTTTATCAACATACATTCCATTAACGGCCACAAAAGATAAACTTAAAATCATCAGCATTAATATCCAGAATACTATGTAAATAATAAAAAACTTAAATTTCAAATAGTTTTTCATTACAATGTTTGACCGTGTTTTTTAGTTTCATCTTTTACTTCAACATCTTTGTTAAAAGCTTCTTCTTCGTATTTATCATAGACGCTTTGACACAATGGATCCATACCAATATATTGTTTAAAAGGTTCCATCATTTCATCCTTTTTCTTTAGTCTCGCTTTAACAACCTTTTCTTTTTCCTCCTTAGGTAATTTTGATTCATCTTTATAAATAATATCTTCATCATTACCTTCAGCGTTTCTATCAACTTTTTTAGCACTATAAATAATATTGTCAGCAACAATTTCCATTTTGTATTGCTTATGATTATCTCTATCCACATAAGCATTTCTCTTTATCTTTCCAATAACTTCGACTAAGTCGCCTTTTTTATTGTACTTTTCAAAATTGCTTGCTTGCAAACCAAAAGCTGTAATATTTAGAAAATCAGGTTCCCCACCATATTCATTATTTACAGCAATCACAAACCCTAAAACAGCTTTACCACTTGTTGTTTTAGCTTTAAAGTAGTCTTCACTTACTACTCTACCCGTTAATATAACTTTATTCATCTGCCTCCTTTATTTTGTATTTTTAACTTTTGCTGTTTCACTTTCTTTTTCTACAATACCAGCAACATGTTTTTGAGCTTCTTTAACAGTTTTATCAGTTATTTCAACCTTATTATTTGCATCAATACTTTCTTTATTATTTAAATCAATGATTTCATCTAATTTTAAAGAAGCTAAATTTTCTATATATTCTTTTTTTATGTCATCAATATCTTCTGGATGAAAATTTAAATAATCATCAATATAAACATATTCTGTATCGATTCTACTTAAATCACCAAGTGAATAAATATATTTACTGTTTATTCCATTTTGTTTTAAATCTTCATCTACTAAATCAAATAAATATTCACTTTCTATATAACCATTTTCACCAAAAAAATCATTAAATGAGAATTTTAAATCTAAACTATTATTTTCTATATTTTCTAAATCTTCGACATTGCATATATTTAATAAAGTGATGTATTGCTCCAAATTTTTTTCATTTGTATCTTCAACATAAAACAATTTATCAAAAATCTTTTGATTTATATCATCGTCTACATTAGACATTTTGTCTACTATAGATAATACTTGTTTTTTATATTTATTCCGCATAGTATTATTCTTGATATTTTTTGCTAAATAGTTAAATTTAGCTACAACTTCTTCATCTGAAATATCACTTAATGCATATTGAGTTAGATAATACAAATCCTTAAAACAAAAAACTTTTTCAATCATTCTTTTTCCTTTCTATTTAGCAAAAAGCTTATTGCATATTTTTTTATTGCTTTCTTTTCTAAATTTTTCATATTTTTCTGGTTCCATTTTTTTGAATTCTTCAAGATATTTTTTAGCTAAGGTACTTTTGATACCATTTTGTTTTAAATCTTCATCTACTTTTCTTTTACTAATCATATTTTCTCCTTGTCTTATTTAATAATTTTTCACTTGATTCTTTTTAAATAAATTATTAATTCTTTCATAATTAATAAGATTTGTTCCTTTTTGCAATTGCTTTAAATAAATTAGAATTTTTATCATTTAAATTGAAAATATTTTCTTTAATATTTCTATAAATTTCTTTATTTGAATTTAATTTGGTTCATTCTGACTTTTCCAAATAAAGCAATGCTATAAGTGCTTCATCTGATTGATCTAAATCTGGCATTTTCGAAAAATAATTTTGATTGTTTTCTTTAAATTTTTTTATAAAACTTTCTATCTTTAATCAATCTTCTTTTTTATCTAATATTTCAAAGAAATCAGCTCCATAATAAGGTCTTAAGTCTACTTGTTCATAATAAAAATTATTAAATAATCTAATATTCTTTATATAATCATTATCTAAAAATCATAAAGCACCCTTAATATTCATTTTTGGGTAAATAATTTTTAAGGCTTCTAATTGTTTAATGATATTCTCACAAGTTTTTTTCATTGCTTTATGTGAATAGCAATCTCTCTTTCTTAAATAAACAACAAACATATCTGTTGGTTCATCTTTTGTTGAATTATTATTCAATCTAAAAATGAAAGATGCATTGATTATTACTTGTTCAGGAATGTCTCCTAAGTGTTGTCCCTCTTTAAGAGGCAAATTGTATCTAAAATTGCTTCTTTGTAAACTAAAACCATATTGAGTAATTAAACTTAAAGCACAATATTGAATAAATTTGTCATAAATTCTTTCTTGATGCTTCAAAAATGATTGTTTAATTTTAGTCTTAACATCAAATGGGTTGGTTGGTGAAACAAAATTAAAAGGTTTATTGATTATTTCTTTTAAAAGTTTTAATGTAGGGTCATTTGTTAAGGCGCTTTTAAGTTCTGCTTCAAATTGACTATATTCCATATTTTTCTCCTATTGTTTTTAATTCTTCAAGATATTTTTTAGCTAAGGTACTTTTGATACCATTTTGTTTTAAATCTTGATCTATTTTTTGTTTTGATTCTTCACTTAATAAAATTTCATCAAATTTATTGTTTTTAATCTCATGCATAATTTTTCCTTTTCCTTTCTTCTTTTCCATAGTTCCTCCTATTTTTTAGCCTTTGACTTTGGATTTTCAATTTTTGTTTCTTTAGCAATTTCTTTATTCATAGCTGCATTTTGTGCTTTTTCAATATTCGCATTATCATATGCGTCAGCTATAATCTTTCCACTTGCTAAATCCTTTTGGTATTGGCTCATCACAGCCTCTTTAGTTGAAACAACAATTTCTTTCATTGCTTCATTAAACGTTTTTGTTATCTCTTTAGCAACACCATCGCTCAACTTAAAGTTCTTTGCAATTTCCGGCTTTATCTCATTTTTAGCTAAAGCTTTTTCTTTTCTTTTATCAAAGAAATATTTAATAGCAGTTATACCTATTACTAAAGGCAAACCGCCCACTAAATATGCTTGAAGACATAAGAACCCTTTTAATGCAAGAGCAGGTAATTTCTTCAAAACTTCCTTAAAGTTCTTGTTTCTTATTTCCGCTTTCTTTTCTTTATCAGCTTCTTTATATTCTTTTACCTCTTTTTCTTTTACTTCATTATTAGTAATAAAATTAGTTTTTCCTTCTACTTCTCCTTTAGCATCATATACAACTTCTTCGCCAGTTTTAAATCCATTTTCATACATTGTGCTTTTAACTAGCGCGCCGTCTTTATATTCTTCAACAGCCCCATTTAATAAACCATCTTCAAAATGTGCTTTATAAAGAATATCGCCCTTAGTGTTATAAATTGTTGCTTCACCATTTAATTCATCATTTTGGTAATTCGCAACGCCAACGAGCTGATTATTTTCAGCAATTTTGTAATAGCGTCCACTAAAAGCTCCTTCGTTCATTTCATAGTATTCTTGACTATTATCATTTAAAACATTATGTGCTAAAGTCAAACCACTTTTTGTACTAATTAATTCAAAATTAGTTCCGTTCTTTTCATAGTTTCTACGATTAATAATTTCTTCGTTTTCTAATGCATTAAATACTTGCATATTAAACCTCCTTAAAATAATGTTTAATCTTTTCTACTCTTTCAACTTGCACCCAACCATAATCTCCTTTTTCTATAATTTCATTGGCTATCTTGTCTGCCTCATTAAAGTTAGAGCAAACTGCATATGATTTTGTTTTCTTAGATTCAAGATTCTTTTTGACTCTAATTCTGTAAAAATATCTTTTCATTCGCCTCCTTCTATGTTTTAGCTCCAGCTGTTTTAATATCATTCATCTGGTCTAAACATATATTTATCGACTTCGTGAAATCGTTCACTAGTTCACTACTTAATTTGTTTTTGTATTTTTGTTGGAAACTTAATATCTTTTGTCAAGCAAGTGCTAATTTAGGCCATTCGCTTATCTTGTCTTTATACTCGCTAAGCTCCTTAATACTTCCTCTTGCATTTTGCATTTCCAAAATAAGTTTCTTTTGAACTTTCTTATCATTAAGTTCTTTATTGAGGCTTTCCTCTCTAGCTTTCTTGGCTCTTGAACTATCATTCAATTGGTCATAGTTTTTAATAGGATTTCTAACCGGTAATTCAACATCTGGCAAGTCTTGATAATTTCAAGATTCATTCACAGTGTTTTCTGTTTCGTCACATTCAAAAACATAATTATCATTACTAAAATATTTGTAAGCTAATTTTGTTTTTACTATCATAGGAAGAAAACCAATTTTTTGAATAATGGCATATTCTTTATTCTTCGCTTTAATTTCCGCCACACTCATTAAAGGCTTTTCTTCCACCGATTCACTTTCTGAAGATGAACCACCATTAGTTGATGTTGAAGATGATTTTGAAGTTGATTTTTTAATAACTTCTTTTTTGCCTAAAGAATTAGATAAATCGCTTAAAGTTTTTTCATCAATAGAGTTCAAGAAATAAGTAAATTGCAAATTACTACGAATAATGCTATCCACATTATGATTAGTGCTTGTATTGTATTTTTTAAGCTGATTGTAATCTTGCAATACAAGTTGGAAAAATACATTTCTACTACGCGATATTGTGACCTTATTATCTAGATTCAATATTGGAGGCAGGTTCCCCGCTTCATCTAACATGAAGAAGAACTTCCGTTCAAGTTTCAAGTCTTCCTTACTATTAGCGCAATCAATTAAAGCTTGATATATTTGGTCAATAAGCATACTTACTAAGAAGTGATTACTTGGCTTATGGTCTGGATAGTGAATGAAGATGACATAAGGTTCATTCGAAGATACAATACCATGTAAATCAAAACTCTTTGTTTCTCTTGTTAATCTCTTTATAAACTCATCTTCAGTAAAGTTTTTTAAAGCACTCGTAGTATGTGATAAATTACCCGTTAATGTTTCAGGAACAATATTTGCAATTGTCTTAACATTTTTAAATAATTCAGCTCAATAATACCTTTTATTTGTTTCTGCATTTATATAGCAAATTGAGATTCATTTTTGTTTAGGATTTTAAGTGTCCAAATTACACATTGAAGCTACCGATTGCAAATTGAAATGCCTTTCTGGTAATAAATCCGGCCTATCAATTGAAAGCAACAATAGAAATTGAGCCACAACATTAACAATCAATTTAGCATTATCAACCCACATAGGATTACCATCGCTATTATTCCCTCATTTTAGCTGTTCAACAACATTATTAATTTCACTTATAGCTTCATTCATGTTGTCCTCTCATTTAGGATGCCCTTCCGGCACTCTATGAGCTAGTTTATAAGCAAATGATAATGGATTCCAACCAACACTTTTCTTTGTATCGCTAAAATCTACCGCATATATTTTGTATCCTTGCTTTTCAAGCTTTTTACCAACCATTTTGATAATTTCTTTTTTTGGATCCGGAACACAGAAATTAGCTCTATATTTATATCTTAGATGTGAATTATAAATAATATTAGGCAATAGTACACGTTGTGTTTTACCTGAACCTACACCCCCCAAAACAACTGAGTTAATATCACTTTCAGGTTCATTCACTCATCATTTATTTAGATTTGAATCGAACTTAATTACCCAATTAGCGTTGTTTTGATTTCCCACATAATTTGGTGCAACTTTAAACTTCTTATCGAAAGTTTCATTACTTACACCGCTATTATCAATTTCATTTCTATATCAATACTTATTAGGTTTTAATAATGATGTTTTAAGTTTATTTTTATAGAATAAATAACTTATACCCGCTATCGATGCAATTAATAAAAATGCGTATGAAATTAAAAACTGTTTTGGGAATGCATAAATGCTTGCTTTTGTCTGTGCAAAAAAGTCTTTAATTGAAACCATCTTAAAACTTTTCAAACGCTTAACATTAAATATAATGGCTAGAGCTAGGAAGATGATAATTGCTGCAAGAAGTATTGCAACAGCACTTAAAGATATTTTTAATATTTTCTTTTTCTTATCTCCCATTATTGACCTCCGCCTTCAGCCATAAAAATATCAAGCTTTTGCACAATAATTCTTTTAGCTAACTTTTGCATTGGAATTGCTTTAGCTATGCAAAGGCATTCTTCAATTATTTGACAATAGCCTTCTACGCTTAAGCCTAAATGTTCAATAAACTTCTGGTCGCATTTATATTTCATTCTTATAGGCTTTATTAATAACATCATGCAAAGGAATACAACCATTAATAATGAAGACGACACCGTAATAGAAATATTTCAAGCAAAACTTATATTTAATACATTCACTAGGCTAGCAAATATGAAGAAAACTAAAATTGATATTGAAAACAATACAAGTGCTACGTTTCTTAATGGCATATTGTTTTTCAAAATCTTTTTATAATGAAGTAAATTGATACCCGTTATAACATCATTTCAATCTTCCGTTTCTATAATTTTTTTAACTATCTTAGATATATTGTTTCCCATAATAAAATCCTATATTTTGTAATAAGTCATAATGTTTTCGTATTCTTGAATGCTTTGAGCTTCAAAAATCTTCTTAGCTCTATTAATCAATCTTCAAGAATCATCATTTACATTCAAGTTATAAAAGAATAATTTTGTAAAGCTTTTAGCGCTCTTAGCATTTCTTGATTTTAGAATTGCTTTTATTATCTTGTTCCCTAGCTGTCTAGAAAACTCATCTTTTTCAGTTCTCAAAACATTATTGATTCTCTTTTTTAAAAACGTTGAATTCGTTTCATCCTTTAGTTGCATAACTAAATCAAATGCCTTTTGATAGTTATCCATCTTTCCTTTAAAATCATCATCATATTTATATAAAAATGTTTCTATCTTGTTTATCGCTTCTTTAACTTCTGGTGAAGCGAGTTTATAAGACTTATTGAACCTATTCTCCATTTCAGCTCTTACATAACAGCAAGCCTCAATAAAGTCTTTGTAATCTTGGCTTTGAAAATTCTCAAGCGTTTGTTCTGTCGAAGATAAAACATCTTTTCTGGCATCTCATAACTTACTTGTTAAATCCTTAAGATTTTGATAATCAAAGTTTTTAATAAGCTTGTTTGTAATCTTCATTCTTAGCATTTTTATATCGTCGATGTTTAATTTTCTTTTTGCTCTTTTTTTAGTCTTTTCAAAAAACATAAAATGGATGTGTGGATTATCTGTATTACCATGTATTGAATAATAAGAACTAAGGTTATCTAAATCAAAACCGCTAATGCTATAGAAGTTTCTAAACTCCTCTTTAACTAACTCAGCTCAATCATTAGCAGTTAAAACATTATGTTCTAAACCTAAATCACCTAAGTTTAAAATGCCTTCATAAAATGTTTGTTCATCAATACTCTTTAATTCTTCCTTAACGTTATCGATATTTACATCAGCACTAGAATCGTTTAACATATCATAAACACCAGAATATTGGCTACCCATTTTAGACTTAAGATGATTTCTATATCAGTTAATCTTTTCTTCTCTACTTACTTCCTCTAAAAGCATTCTATCTTTTTCACTATCATCAAAAGGACTAAATGCTACACACTTATCTCCTCTTGCAAAATAATCAATAACTGCCCCGCTTGATACTCAACTATTTAAATCATGACAATAGCCTAAACCAGTTTTATAGTTAGTCATTTTAGATACATAGTTGAACTTCATATAAACATCCATTCTCCCTCCTCTTTAACTAACTAACTTCCTTCTTTGTATTTAATTTCTTTCTCCTTCGTATTAATTCATTACATTCATTAATACTCCTAATTATTTATCGACCTAATTTGGACATCATCAGCATACTTATTCTAAAATGTCTTTAAACCCCAAAAAGGTTAAATATGAAAGATAACACACGAGTGTGCGTCCATAAGATTTTCTAAAGCAAGCAGTAATGAATAAACCGCAGTAATAATTGAAGCTTATTCATTACTACTCCTCATTTGCTTTATATCTGCATTACAAGATACCTTTGAGTCTTAAAGCTTAAATGCTTCAAGTTATCAACACTTCTTTGAATAATAAATAACTCATCAGTTCTCAAACCTAAAACCAAACATATATTTTTCAAGAATCTGTAAGCCGTAGAACCTTTTAGCCCTAAAAAGTTATTTTGTACAATGACTTTCATCTTTCTACTTTTAACAAACTTTTTAGTTCCAAGTTCTTTTGATTTTCATACAAGCGTTTTATAAGCTCATAAATTAGCTAATCCAAACAACAAGAAAATCTTCCAGTCTTTATCACCACTAATAACAATGAACTTTCTATCGTCATTAGTTAGCTTAAACTTCTTAATTTTCACAGCGTTTCTAACTTTTAAAATTAGCCCTATATCTGTTAAAAACTTTACCGCTCTTTGCAAGCTTGTTTTCTTGAAGCCAAAAGCCACTAATTCATAAAAATTTACATAGCGTTTTTTAGCCCCAAGAAACCTTAAACAGACATACACTTCAAAAGCAACTTTTTCAGTTCTCAAAATCTTATAGTAGCTTTCACCGAGTACTATCTTTGTTTTTAAAATTTCACTAACTGTATCTTGGCTTAACTTGTCATAAACCTTCTTGCCAAAATAATTTTTTCTCCATTCCAAGTTAGCTTCAAACTTTCTAACTTCGTTTTCGCTTCCTTCATTTGCAATTAACCTTAGTTCGCCGTATACACTCATAATTTCTCCTTTTTTTTGAACATATAACAGCGACTGTGGTATAATATATACAACAGGTCGAACGGGTAGTAAGTGTTCTAGACGCTGTTATTTTTTTATACTTTTGATCATTTTTTTGAAAAGTATATTAATTTAGCAGAATACGAAACTAAATAAAAATATGAATTAATGTTTATGAGTACTTTTATAAATAATCAAAAGTGAAATAAAAAAAGTGTTCAACACACTTCTTTCTATCACTTCCCCAAAAAGTCCGGAATTTGGGGTTCACTCTAAATCCCTTACTATTTTTTCTTATCAATATTAGTTTTAAGAATTGATAAAAATGCTTCTTGAGGAACTTCAACAGTACCAAGCATTTTCATTTTTTTCTTACCCGCTTTTTGCTTTTCTAAAAGTTTTTTATATCTTGAAACATCAGCTGCATGAAGTTTTGAAGTAACATCTTTACGATAAGCTTTAATAGTTTCTCTAGCAATAATTTTACTTCCAATAGTTGCTTGAACAGGTACTTCAAAGTTTTGTCTTGGTATAGCTTCTTTTAATTTTTGAGTTAAATCACGAGCTTTACTATAAGCTGAATCTTTATGAGTAATAATTGAAAAAGCATCAATTTTATCACCATTTAAAAGTATGTCTACTTTTACCAAATCACTTGTTTTATAGCCTATTAGGTCATATTCAAATGATGCATAACCTTTAGTTAGACTCTTAAGTCTATCAAAGAAATCAAAGATAATTTCGCCTAGTGGCATTTCATAAATAAGCCTTGATCTATTAGCATCAATATAGTCCATATTGATATAAATTCCACGTTTATTTTGACATAGTTCCATAACATTTCCTATGTATTCATTAGGTACCAAAATCTCAGCTTTAATGTAAGGTTCATCAATATGATCGATAAAAGTTCGATCAGGTAAAAGTGCAGGGTTTGATATTTTTTTAATGCTGCCATCTGTCATATGAACATCATATTCAACTGAAGGACTAGTTGCTATAATACCGATTTTATATTCACGATTTAATCTCTCTTGCAATATCTCCATATGTAACATTCCAAGGAATCCAACTCTAAAACCAAATCCTAAAGCTTTAGAAGTTTCTTGTTCTCAAGTTATTGAGCTATCTGAAAGAGCTATTTTTTCTAAACTTTCTTTAAGTTCCATATAGTCGCGAGTATCAATTGGATAAAACCCAGTATAAAGCACAGGTTTTTTCTTTTTGTAACCTGGCAGTGCTTTTTCAGCTGGTCTATTAACTAAAGTTATAGTATCTCCAACGCTTACTTCTCTAGCATCTCTAATAGTAGCTGCTACATATCCAACTTCACCTGCTTCAAGTCTCTTTTTCTTAGTTTCTTTTGGACTTCTAACTCCAAGTTCACTAACAAAATATCTTTGTCCATTCGACATAAACATAAATTCATCGCCAACTTTTAATTCACCTTCAAAAATTCTTATAAGCATAACTACACCACGATATTCATCGAAGTACGAGTCAAAAATTAAAGCTTTAAGAGGTTTAGAATCATCAGCATCTTTAGGTGCTGGAATATATTTTTCAATTGCAGCTAAAACTGCTTCACAATTTAAACCAGTTTTAGCTGAAATGCAAACTGCATTATCAGTTGGTAGACCAATCACTTCTTCTATTTCATTTTTTGTGGCTTCAATATCAGCTGAAGGTAAATCAATTTTATTAATAATAGGAATAATAGTTAAATTATTTTCAAGAGCTAAATAAACATTAGCTAAAGTTTGAGCTTCAATTCCTTGAGTAGCATCAACTAATAATAATGCTCCTTCACTAGCAGCTAATGAACGTGAAACTTCATAAGTAAAATCAACGTGACCTGGTGTATCAATTAAATGGAATACGTAATCTTTATACTTAATTTGTACAGCATTTAATTTAATTGTGATTCCTCTTTCACGTTCTAAATCCATTTGGTCCAAAAATTGATCATCCATATCATGGTCACTAACTGTATTAGTAATTTCTAGAATTCTATCAGCAAGCGTACTTTTACCATGATCTATGTGAGCAATAATTGAAAAATTTCTAATTTTGCTTTTGTCCATTATCTTCACCTATTTAAGTAAGCTAAATTTTATATTAAATAAATAATAATATAATATATAATTTTTGAAATAAAAAAAGGAGTAAAAATGAAAGTTTTAATTAAAAAAGGACATACTAACATTTCATATCGAGATGAAAATAAGTTTATTCAAGAAAAAATTTTAAATAAATTTAATCACAAATTAGAATACAGTTTATTAAAAGATTTTAGTTTTACACCAAAACTTATTAGCCAAAATGACAAAGAAATCACTTGAGAATATATTGATGGTAAAGAATACTGAAATGATGATAATAATATAAAGGAAGTAGCTCAAAATTTAAAAATTTTACACAATTCAAAACTTAATTTTCCTGCTTCTAATCATGCAGCTAGAATTAAAGAATATCGCAAAATTATTCAAGAAAAGAAACTTAAAATTAAAGTGTTGAATGATTTTTATAAATTTATTAATATGACACTTTCAAAAATGAGAAAAGATACTCCTCTGCATAATGATCTCTGATTTTTTAACATGATAGTTAAAAATAAAAAAGTATATTTTATTGACTGAGAATATGCTTCGCTAGGTGATAAACATTTTGAACTTGCTTATTATATTGAAGCAAATGAGTTAGACAAAAAACAAGAAAAATTATTTTTAGATACCTATGGCGAATATAATTTAGTTTATCTTTTAAGACACAAAATATTGGTTAATTATATCGTTGTTTTATGAGCAAATGCTCAAAAAATATTGCCCTTTGAAACTACTAAATATGAGCAAAGAATTTATGAATTATATAAACAATTTAAGGACCTGCTTGCAAGCTAAAGCACTATAAAACAATTGTTTTAGTGAAATTAGATCATTTTACAACCTAAAAAAATATTAAATAATTTACATAGCAAAAAACAAGAAAAGTTGTATAATAATTACATAGTTTTATATATTAAAAACTATAAAATTTGATTAAAAAATGAGGTGTTTATGTCAGATAAAGGCAAAAAAATTAAATACGCTTCTGTCATTACTTTGTCAATTATTTTAGCAATATTTACTGTTTTATTAATTATTGTTGCTAAAGGAAGTTGAATGGCAAAAGTAAGTGAAAAAAGTGGAGATGCAGGTAAGTTTTTAATAGCAAACAGACTTGTAACAAATGGAATTTTTGCAGACTTTTCAAACTTCACAAGTAATTTTTCAATAATTAAAAGCGTAGTTGAATCAGACACAATGGTTTATATAGCTTGTGGAATCTCAGCATTGTATTTTATCATTTATATTTCATGAATCTTTACAGTGTTAGCAGTTACAATAAAAGCATTCGTAAACAAAAACTGATTTATAGCTCCAATTATGCTTGCTGTATTTGTAACAATGTTAGTTTGATTAGTATTCCAATTCTTTGGTGGAACATTCAAAATGACAAAAGAAAGCGATGAAAAATTCATCGGTGGAGTAATACCATTTATCCAATTCTTTACACACATTGGTGTTGTGGTAGTTTCTATTGTTGGTATTATTATTGAAAGCGTTAAAGGTAAAGAAAAGAAAAATGTTTGAGAAACAACAAATTCTTATAATGCTGAACAACAATAATTTTGATATTAATAAAAATAAGAGTGGTTATGCCACTTTTTTATTTTGCACTTGATTTTTGAAAAAAATAATTATAAATAATGCACTTTTTCAAACAATGATTTATTATTAATATTAAGTTTTATATTTATAATTAATGTACATATATAAATAGATGAGGTGGTCAATATCATGTCAAACAATGAAGAAAAACAATATCAAAGTATTCTAGACAATTTACTAGATGTTAGTCCAAATGACAGTGCAGTATTTACTAGAGTAAATAACAAATTTGCTTTTGATTTTTATACTATTTTTGGACCTGAAAGTTTTAGCAAAATTTACAACAATAAAAACTTTGACATACCTATTCTTGAAGTTAATTTGATCAGAATTTGTAATTTACTTGAAGAAGCAAATACATATGAAGAAATTATTAAAATTCTTGAAGAAAACAATATTGAAGTAAGTGAAAAACGTAATAGTCAATTAAAGAAAAATCCTCTTGGAATTAAAAATAACATTATTAATGAATTAAAAGTTAATTTTCAAAAACAATCATTAAAATGAAAGTTATTCTTAAATAAAGCTAATGAAATTAACACTGAAACTAACATTTGACCTATGCACTTAGGTTTCTTATTTGTTAAATTAGTTATTGACGATAAAGCTGTTTATGGTCCTTTATTTTTAAAAGAAGTTTATTTAGAAATTAGAAATGCTAGACCATATTTAGTTTCAAATGGTGACATTAAAATTAATGAAAAATTGCTATTCTTATTAAGTAATGCAGGTTTTGATTTAAATATCTCGGAAAACTATGGTGATTGATCAATTAAAGAATTAATTAAATATTTAAATGAAGAATGAAAAGATATTTACAACTTTAACTTAGACTTAAATCAACCTTTCAAACGTTGAATTCCCGAAGCTATTGGCAACCAAGCGATGGAATTTGAACCAGGCGTAGTTTTAGGCTTATTCCAACCAAGTGGTGGCTATGCCAGAAATAGAATGCTTGAAATAATTAAGAATAAAGAATTGAACAAAATTTTAGATATAGAATTTAACAAAAACATCTATAAAAATAGGGTAAAACAAACAATTTACAACCCTAAAACATCTATTTTTAAAATTACACCTACCAACTATTCTCAAGATAAAGCTATAGCTTCAAGCTTAAATCAAAACACAATTATTTGAGGTCCTCCAGGTACAGGAAAAAGCCAAACTATTGTTAATATTTTGACTAACCTTTTAGTTTATGGCAAAAATTCTTTAGTTTGTTCTCAAAAGAAAGCGGCTCTTGAAGTTATTAAAAATAGAATGGGCGCTTTAAAAATCTTTTGTCTTTTCATGCTTAATTCAAAAAACATGAACAAAAAGAATTTCTACTTGCCTCTTAGAGAATATTTAGATTATTTAGAAAACTATGATGATAATCCTAATTTAAAACCATTAAGAATCATTAACCAAAAAGATATTCGTTTTGTTAACAATATAGCTAATTTCAGTAGTGATCCACGTTTTGTTAATATCACTCAAGCTTTAGCTAAAGTTCATAATAATTGATCATCATTTTCACAAGAAATCTGAAATGCAATTTTAAAATTGCCTAAAAATATTTTATATCCAGCCGAATTTCACTTTGATGATTCAAAAGCTTTAACTAATGACATGCTTAGAATTAACAAAATGAAATTCAAATTCTGACATTTCAAAAACTGATTAATTAAATTAACAGCCCAAAAAATGTTTAACACATTTAAAGAATTTCAAGGAAATTTAAATGATTCAGTTCAAGTTTTAAAAAACTTAAAGCAAGAAGACTTTGAATTTTTAAATGATTTAATAAACATTTTGCCTTCTAATAATACTCAAGAAACTAGCGATGAAAATGAATTAAAGAAATTTATTGCTCAAGGTATTATTAAACGAATTAACAAATTTACTGATGAAGAAAAAGATCAATACTCAGAATTTGCAGCCACAATTAGATTAGGAACATTAGAACCTTACAAGTTTATTAAGCGTTTTCCTAATATGATTAAAAAGATTTTCCCTATTGTAATTGTGACACCTGAAGCTGACTTAAGTGCCTGAAATAAAGGCGAATTTGACTATGCAATCCTAGATGAATCAAGCCAAATTTTCCTTGAAAAAGGTTTACCAGTATTATATTTAGCAAAAATTAAAATTCTTGCTGGTGATGATCAACAAATGAAACCAAGTAACTGATTTGGTGTCAGAGTTAGTGATGACGAAAGCATTTATGGAAAAGTAGATAGCCTTCTAGACTTTGCTAAAAGTCTTGGAGTTTATAGTGTGCTTTTAGACAAAAACTATCGTTCAAATTATGCTTCACTGATGACTTTTAGCTCTAAATACTTCTACAAAAGTACTTTGGATGTTATCGATTCAGCTAATATGGAAGCTAATTTCAAGCCAATTGAAGTACATGAAGTTAATGGCCTTTGAGAAAATAATCAAAACACAGTTGAAGCTGATTTAGCAATCAAACTTATTAAAGAAAATATTAATAAATACAACAAAATCATTTTGTTGTGCTTCAATGCTAAACAACAAGATTCAATTACCACAAGCATTTTCAAAAATCATCCTGAATTAGAAAAAGCAATTAATAATGGAAACTTGTTATTAAGAAACATTGAAAATATTCAAGGCGATGAAGCTGATTTAGTTATAGCTTCAGTAGCTTATGATAAAGATACAGCAATTCACTCAACTTATGTTGGACGTCCTGGCGGTATGAATGCGTTAAATGTGGCAGTTAGTCGTGCTAAGGATAAAATGATTGTTATTAAATCACTTAAATCTAGTGACTTAACTATAATGACAGGTAATGAAGATGCTATTATGTTCAAACATTGACTTGAATTCTTAGAATTAAGTGACATTGAAAGAAAAAACTTCTTACACTTAGAAGATGAAGAACAAAATAATAATCAATTGCAAACCATTGAAACTGAACCAGTTGAAGTTGAGAATTCATTATTAAATCAAGAAATCAAAAGCACAATTCTTGAAGCAATTAAAGATAAACCTTATCTAAAATTAATTCCAAATGAAACACTTGGAACACTTAAAGTTGATTATGTTTTAAGAAGAAAACATGAACCAATAATGACATTTATGATCGATGATTACAAATATGTTAATAATCCTGAAGACTTTGTTATTTTTAAAGACTTGTGTAAATTTATTAGATCTAAAAAATATATTGTTTATAAACTAGATCGAATTTTATGAGAAGATAAAAAAGAAGAAATTCTTGAAACTATTAATAGTATTGAACCAGTTGATATGACTCAAGTAGTTGAAGTAACTAAAGAAATACCAACAACTCAATTAGAAATTCTTGAAGCAACAAACCAAGAAATTAAAGAAGCAAATGAAGTTATTGAAGAAGAAAATCAAGTTAAAGCAATAATAATTAATGAAAATAGTCAAGTAATTAACGTTAATGAAGAAGACTTTATCAGTCAAGAACACTCAGATACTATTATAGTTGAAGAAATTAAAGAAGTACCAGTTTTAGATAATGAAAAGAAAGATGAAACTATTGAAGAAGTTTTAGAAAAAATTAGCCAAGATCGTGAAAACACCAAAACTCAAGAAATTGAAAAATCTAAAGAAAATACAAAAGTTGTTGAAGCTATCAAAAAAGAAGAAACAGCTCAAGACACAATTACTTTGAATGCAGAAAAAACTCAAGAAGTTGGTTTTATTAAAGATGAAGCAACTATGGTTATTGAGCTTAATGTTGAAGAACAAAATGAACAAATTCAAAGTGTTTCACATTCGACTGAAAACACTATAAAACAGGGAGAAACAAAAAATATTGAAGCTGAAAATGCAATTGAAGAACAAGAAAAAACACTTGATCAATTAATCGAAGAAGGCGATAAAATTAAACAAAGTGATTATCTTGAAAGAGAAGAAGTTCCAGTTAAAGATTTTGAAGAATCAACAAAGGAATGAGAATCAATTTTAACTCAAGAAATTGAAACTAAAGAACAAACGGAAACTCAAAATTTAAATGCTAATTAATAATATTATCTATTAATTTGAGACTGTTTAGAATTTTGTGTAAAGTTTCTCCATTTCTCAATAAAAAGGAATTAACTTTACATCAACACCTAATAATATTGTTGAACAAAATAAGATAATGCCTAAAAACATTATCTTATTTTAAAAATTTGTTAATTTCAAAAGTGAGACCCCTAGGTTTTAGAGATTTCACTTTTGAAATTAACAACTATTAAATGCATCTCTAATTTTGATGAAATCCAAAAGTATATAGGATTGTTGTTTTTCAATGTTTTAATAATAAAACTTCAATTTTTTTCTAATTTATCAATGTTTTTAATAAATTCTTTATATTATCCAACTCTAATACCAGTTGAAATAAACACACGAAAAATGATACTAAATTTAGTGTTTTTGTGGCTTTTTAATTCTTCAACAAGCTTAATGAATTCCTTTTCAAGTATAGCTTGTCTTTTGTCTTTTTCTTCTTTTATATATAGAAGTTCATTTTTAACATCTGTATTTGTATTAGTGATTTTGCAAAATCAATTTAAAAAAGAAGAAAATATGTTTCTTTTAGTTAATTTAGTATTATTTGAAATTTTATTATTTGCTAATTCACGATTCATCCTTATAATTAATTGATTCACATTGTTTCATTTTTTTAAGGATGATAAGTACCTTTTATATTGTTTAAAAGTTTGATAACTTCTTTGATTTATTGAACCATTGAGTAGATATTATTCAAAAAAATATCGAGAAAATTATTATTCTTTTTCATGTTTTTTAACCTTTAAAGTGTCCAATAAATATTTTAATATAATGACATAATTTTCCTTATGTCCCGAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAGCATAAAATACTTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAATAAAGATGGTTTAATTAAAAAATTCATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATTGAAACAACAGTTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGAGACAAGTATATTGAAGGCAGAAACAAATTAATTAATGATTATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATTGATTTAAAAAAGCCTTTGTTAACATTAAATCAAGATTAAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAGCATTCTTTGAAATAATTTCAAATCTTAAAAAATATGAAACAAATTATTTAGAAATATTTAAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAAAATTGTTGATTTCTTATCAAAAGTTTTTGCTAATTTAAAAACATTAAGCCAATTTGAAGATGTTAAAAAAACAATTGAAGATATTGATAATAACAAACCTGATTTATCAACTAAAGAAATATCTTCAATTGAAAACAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTCTTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCAAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTACAAACACTAATTTAATTAAAAATTATTTAGAAAATGATTATTTATTAGCTGTTAAAGATTTAAGAGAATATTTCATAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATCTGTCTCACAAGATAATGCAAATTGAGATGAAAAATCAAAAATTTGAACTAAACCTAAAGTTCTAGGCAAAAAACATCTATATGACCATATTTCAGATTTAGATACTGGAGGATACTCAAAAGGTAACACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCCGAATATTCATGACACCGATATGGAGTAGATAAAGGAAATAGTACTAGTGAAATCGAACAAGGTAAGCAAATTGAAAATCAATATTGAAGGCCTTGACATACAATTATTGAACCTCCAGAAGATAATAATCTACCACTAGATTATAGTATACCAACAGTTAAATATATAAAAGAAGCATATCCTAATAAAAATAATGGCGATTATTTAAAAGCAATACAAAATGATATTTGAAGTGGACATGATGATCTATATACTTTAGCCAAATTAATAGAATATGAGAAAACAATAACTGAAAAAATGCTTTCAAATATCAAAAAAATGTCAGATTCTAAAATAATGTTTAAAGATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGAGAAAGATGAAGTGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTAATGTTTATAACAAAAAAACAGGAGAAGAATATCCTATTGGAGTATTACCAAGTAATTTCTATGGTCCTCACTATATAACAAAACGTGGAAAATCAGATGAAAAATATGATAGTACTAACCCACTATTTGAATATATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGAACATAATTCAGCTTTCATATTACAACATAATGGAGCTCCTATTTCATGAGTAAATTACATTTTTAAATTTACTCTTGATAACCCATTAGGAAACTTTGATGCTTCATACTACATATGAAATAAAATGTACTGAAAATCAGAATATGCAATCACCGAAAGATACATTTTGAAAAATAAATCTTCTGAAACAGTTTCAATTTGAGATAAATGATCAAAAATCTTTACTGAAACAAGTAATAATACATACAGCAAACTTATTTGATAAATTAACGAAATTTAATATTATGCTGAACCCCAAATTTCTTTTTCAAAAAGGGGTTCATTTATTATTTAAATTAAAAAATATTATTAAGACTATTTATAATTTTGTGTAAAGTCTCTCACTTTCTCAATAGAAAAGAATTGACTTTACATCAACACCTAATAATATTGTAGAACAAAATAAAATAATGCTCAAAAAACATTATCTTATTTGGTATGAACCCTTTTCCAAAAAGTCCGAAATTTGGGGTTCACTATAATTTTTAAACTTCGAATTTTATTTTTTTAATTTGTTAAAATTAAGATATGAAAAATTTAAAGTCAAAGTTACTTCAAAAAAATATTTAAAATTTTTACCTGAAAATGAAACGTATAGATTCATAGTCGATTATATCGACAATAAAACAGAAATTTTTTTAAACAACTCTTCAATTGAATTAACTGAAAAAATGTTTGTTTCATAGCTAACATTAACTCGTTTTGCTCGAGCAGCTAGTTTTATTAGTTTTAAATCTTTACAAATCTATATTACTAAAAGAACAATAGTAACAGATGATTGAACAAGAATCAATGTTGGTTCAAATTTAAGTATGCATGAAATAAGTAAAAACATCTTTGCTTACTATACTTCAAGCTTACAAAAAACTTATGAGTCAATTAACAAAGAAGAAATTAAAGAGTATTGCAATTTATTAAGCGAAACTAAAAATCACATATTTTTTGGTATTGGGCAAAGTGAAAAAGTAGCTAGTTATTTACGTGAAAATTTAAACAAAATTAGACTTACTTCATTACCTATTAACAATATGCATGATTTTTTTAACATAGTTTATGTTTAATGGGTCCAGATGCTTTTGTTACAGTTATTTCGCATTCTTTTGAAACAGCTGAAGTTATAAAAGTTTGTGAAATTTTGGAAAACAAGAACATTAATTATGCTTTATGAACTCAAAGCACAAGTATTGCAAAATTAAATGCTTTAAGTATTTTAAATTTAACAAATTTAGCTCAACATATTAAAACTTCTGCTTCATTAGGCTCAAAAATTAGTGTTTACTTTTTAACAGATTTAATCTACTTTTATTTAATCTTTAGTTTAGATCCTCAACTTGATAAATTTTATAAGATTAATTTTAAAAGAGATTTCTGAAATAGTCAACAAAGAAAAAAATATTCAAAAACCAGTGAAAAAACAGTTAAAATTATTAAAAAAAGGCAAAAAAGTCAGTAACTTAGAAAATCCTTTTAATAAATATTAATTTATTAGATTAATGCAACTAGTTTGCATTTTTCTTTTTTAATTTTTGACATTTTTCAGCCAACTTATTTTTTTAATATATATATTATTTATTAAAAATGCAAACAATATATTTAATATATAGTATTATTATTTAGATTAATATATAAGGAGGAAAAAAGATGTTAAAAGTTTTAGATCATCCGCTTATTTCAATTAAGCTGACTAATATGAGAGATAAAGAAGCAAATCACTCTCGTTTTAGAAGAAACTTAAATGAAATCGCATCATTAATGGTGTATGAAGTTTTAAGAAACTATAAAACTAAAGCTAGAAAAATAGTGACACCTTTAAATCAAGAATATGTAGGAGCTACTTTTGATAAAGAAATAGTTATTGTGCCAATTTTAAGAGCTGGGCTTGGAATGTTAGATGGCTTACTTGAATTAATGCCTGAAGCTAGAGTTGGTCACATTGGACTTTACCGCGATGAAGTAACTCATCAAGCTCATGAGTATTTTTACAAAATGCCAGAAGTTAAAAAAGATAGCTACATTTTTGTAGTTGATCCAATGCTCGCTACTGGCGGTAGCGCAGTTGATGCTATTAAAAAATTAAAATCAGATGGCTTTACTAATATTCATTTAGTGTGCTTAGTTGGAGTTAAAGAAGGAGTAGCAGCTGTTGAAAAGGCTTTTGGTAAAGACTTTGAAATTTATTTAGCTGCTCTAGACAAAAAACTCAATAATCAAAAATATATTGAACCTGGTTTAGGCGACGCAGGAGACCGTATTTTTGGAACAAAATAAAAGTATTTATTATGGCAAAAGTATTGTTAAAAAAATAGTCCAAACTGGAGTTTTATTAGCTTTAGCAATTGTAGTTAATATTACTTGCTCAAGCTTTATGAAATTTCCTTTAGCGCCTTTTTTAAAGTTTGATTTTGCACTTGTTGTCGTTACTTTTAGCGCTTTAGCAATTGCACCTTGATCAGCTTTAGTCTTAATATTACTTTTAGCAATAATAGGCCCTGCCTATGGCAGCAATGGTTATGATCCTATTGGTTTATTAGGACATTTCCTTTTAGCTTTAACTCAGCTAACTTTTGTATTAACTTTTTATTTTCTATATAAGTTTTTTAATAAAACAATTTATCAAAATTCAATTCGAAAACAAATAGATTTTTATATTGCTGAATTATCTTTAGCAGCAGTCATTACAACTTTGTTTTTAACTTTTGTTAACGTTTTTATTACAACACCTTGATACTTTAAAGCTTTTGGCGCATTAAAAAATGAACCTGCCACAATTAATTCAATGATAAAAAATTGAGACAAATTCAAAGGTCTATTTCTTAATATAAACAACTATTATTTAGGCACTAGCACTATTTTTGCTCTCTTCAATATTATTAACTTAAGTTTAAATGCTTTAATAATTTTTATCTTTTTAGCCTTCAATATTAAAGCTAAACTTTGAAAATCCGATTTACTTGAAATCAATAAAAAGAAACATTTTAAAAATATTTTTATTCAAGCTTGTTATGCAAAATTAAATGATAATCACAAATAATTTAGACATCATTTTAGGAGTCAAAATGTCAACATATAATCAACATAAAGTCGAAAAAAAATGACAAAATTATTGAGCTGAAACTCAATATTTTGAACCACAAAAAACAACCAAGAAACCAAAGAAATATATCTTAAGTATGTTCCCTTATCCAAGTGGAAATATTCATATGGGGCATGTTAGAAACTATGCAATCGGCGATGTTTTAGCACGTTATTATCGTCGTAAAGGTTTTAATGTTTTACATCCTTTTGGTTGAGATGCTTTTGGTTTACCAGCTGAAAATGCAGCTATTAAAAATGGCATTCACCCTAAAGATTGAACATACAAAAACATTAAAAATATGAACCCTAAATTAAAGGATTTGGGAATCTCTTTTGCTTGAAATTATGAATGTATTACAGCTGATGAAATTTATACAAAATGAGAACAATTGATTTTCATTAAGATGTGAAAAAAAGGTTTAGTATATCGTAAAAAAGCATTATTAAATTGATGCGAAAATGATCAAACAGTTTTAGCAAATGAACAAGTTGAAGATGGAAAATGTTGACGTTGTGGCAATATTGTTGTTCAAAAAGAAATGGAACAATATTACTTAAAAATTAGAAACTACGCAGAAGAATTACAAAATGATTTAGAGTTATTAAAAAATCATTGACCTGACAAAGTTTTAATGATGCAAAAAAACTGAATTGGCTATGAAACAGGCTTTTTGGCAAAATTTGACATAGTTGAAAATAATCAAAATCACTTTAGTAATATTGATATTTTTACAAAAAACAAAGATATTTTAGTAACCATGAATTTTATAACTATTGGTGCAAATCATGGCCTTGTTAAACAATTAATTGAAGAAAATAAATTAAGTGATAAAGAAATTGAAAAACTTAATTTAATTAAAGCAAAAGCTACAGCAAAAGATTTTTCGCAAAAATTATGTTTAGCTTTACCACTTCAAGCAATTAATCCAGTAAATAATCAAAAATACAAAATTTATGTTAGTGATTTTTGTTCTCTCGGAGAAAAAAATAGAAGTCAAATTATCGATACTACTAAAGTTAAAAGTCATGAAGAATTTGCCAAATTTAATAACTTAGAAATTACTAATTATGCAAATAACAAAGCTGATTTTAGTAAGCTTGAAAACTCGAGTAAAATCAACTTACAAGATTGAGGAATTAGTCGCCAAAGATATTGAGGCGCTCCTATTCCTATGATCAATTGTGCTAAGTGTGGTTTAGTTCCTGAAAAAGAAGAAAACTTACCAGTTACTTTGCCTTACAAAGTCAACTTTACTACTAGTGGCAACCCACTTAAAACAAATAATAAATGATTGGAAACTAAGTGCCCTAAATGTCACTCAAAAGCAACCAGAGAAACTGATACTTTTGATACCTTCTTTGAATCAAGCTGATACTTTTTAAGATATACGACACCACCAGCTAAAAGAGAAAGCCATATTTTTGATAAAGTTCAATTAAAATACTGAAATAATGTTGATGAATACATCGGTGGAGTTGAACATGCTATTTTGCACTTACTGTATGCACGTTTCTTTACTAAAGTTTTAGCAGACTTAAAAATGGTTGACTTTAGAGAACCTTTTGATAATTTATTAACTCAAGGAATGATTCTTAAAGATGGACTTAAGATGTCTAAATCAAAAGGCAATGTTGTTAGCCCAGATGAAATGATTAAAAAATATGATGCTGATACTATGAGGCTTTTTGTTCTTTTTGCAGCACCGCCTGAAAAAGAACTTGAATGAATTTCCTCAGGTATTGAAGGTTGTCATAAATTTATTAAAAGATTAATAGCAAGAAGCAAAGAAGTTAAGGCTAAAGACAAGTTAGCTGATATTAAAAATCTAAAATTAAACAATGAAGAAAAGAATGCTAGAAGAAAACTTTATCAAGGTTTAATTAAGCAAGAAAACTTGTTTAGTGATAGACGTAATAATTATGCTTTTAATACTATAATTGCTTGAGCTATGGAAACTTTCAATGCTTATGATAAGATTACTAACTCATTATTAATTAAAGAAATGTACTATGTTTTATTAAACATTTTGGAACCATTTATTCCACATTTAGCTTGAGAATTAAGTGAGCAATTCTTTAAGCTTAAAAACCTTAAGGACTTTAGTGTTGATAATAGTGCTTTAGAACATGATGAAATCACTTATGGAGTAACAATTAATGGCAAAATGAGAGTTCAAATTACAGTTGACTTAGCAAACAATAACAAAGATTATGTTTTAAATTTAGCTAAAAATGAAGCTGCTAAATGATTAGAAAATCAAGTTGTTATTAAAGAAATTTTTGTGCCAAATAAAATTGTTAACCTAGTTATAAAGGCTAAATAGAGCCAAAAATCACAAATAATTCCTAATTTTATTTATTTATTAAATAAATGTTAAGAATAGATTAAGAAAATATAAAAATATTTGGCACTTTAGGCATAAGAGTGCTAAAATATAAATTATGTATTTTAGAAGGAGACCATATGAAAGAAAAAATCTTACTGTTGGACGTCTTAGATAAAGAATATTTATCTGACGCAGTTTATAATCATGAAACAAAAATTTTAGAAATAAAAACAAAAAACCAAAGTTTTAGTGATCAATATTTAAATCACTTGTTTAATTATTTTGAATCAATTAGTCATGATGAAAAGAAAGAAAAAAATGACCAAGCTAAATATTTATTTATTGGTTATTTTGACTACGCAAAAAACAAATATGATGGCAGCGATGAAAGCAGAAAAAAAATAGTTACAAAAAGTAACAATGTAAACAAATATTGTTCTTATGTTAGAGTGCTTGATTTAAATATTGAATACAAACAAGATTCAAAAGAAATCGATAGATATAAACTTAAAGTAAAAGGAATTGGACGTTATAAAATTACTCATTTAGAAGATAATAGTGAAAAAACACCTGAAAATTCATATAATGTTGAATTTGCAGTTGCTTCAAAAAGTTCTTATGAAAATAAATTAAGTAAAGATTCAGTTAAAAAATTAAATGTATTAGATAAAACTATTACAAACTTAGTTGAAAAAGGATTATGAAGCAAAATTTTAGATCATAATTTAAGCTTAAAAGAATCAGAAGGAGCACAAAGTAAATGAATGGAAGAAAGCATGTTTAACACGCCTCGTTGAGATGAAGATTATGATGAATCTGGCGAAAATATTGTTGAAGCCCTTTTAATTTATTTTGCTTTAAATAGCCTTTATAAGATGGATATCTTTAACTTAGATTCAGTTAATGACCATATTGATTACTTGTCATTATTACTTTATTCATATCGTAATTTTGGTTGAACTCCAAAAGAAAAAGGGATCATTAATTCACTTAAATTTTTTGACGAAAACTATACTAAAACAAAAAGCATTAACACAAATTCAAAAGCAGCTGTAAAATACTTTGAAACAGAAGCAATTTTTAGAGCTAATGAAATTAAAGAAGTTGCAAAAGAATACAACGAAGAAAATGAAAAAACTAAAAAAGTTCAAGAAAACAAAAAAAACTTATTAACTAAAAAACCTAAGAGTGAAAAAGATTCTAAAGATCCTATTGCTGAAGCTTTATTAGAAAATGAAATAAACCAAAAAGTTAAAAGTAATTTAGAAAAACAACAAAAAGAATTTCTTTTAAGAGAAAAACTTAAAGCTATTAAAGAAAGTCTTTCAGAAAATGATTCAGAAGGTGATGAAGAAGATGACTCATTTTCAAAACTTATTCATGATCCTAAATTAAAACAAATTTATCCTGAAAGTGTTTTAAAAATTATCTCTACAGAAAAGGAAAGACTTAAAGGTATGATGTCAGGAACACCAGATGCTAATGTTGCTGCTACTTACATCAGCACATTAAAGAAAATGCCTTGAAGAAAAATAGAAGTTGAAACTTTAGACATCAAACGTGCCAAAGAAGTTTTAGATAAAAATCACTACGGCTTAGAAAAAGTTAAAGAACGTATTATTGAATACTTATCATTAATTATTAACCATAAAAACTTATCTAAAAAATCAGACCAAGACAAATTAATTGATATTGACAAAAATTATCAAATTGATATGTCCTTATTTAAAGAAGATGAAAAAAATGCTAAATTACAAACAGAATTTAACAATGTTCCTATTCTTACTTTAGTTGGTCCTCCAGGTACTGGTAAAACCTCTTTAGCCAGAGCTATTGCTGAAGCGCTTAACAAAAGTTACATTAAAATTAGTCTTGGTGGTGTGCATGATGAAAGTGAAATTAGAGGTCACAGACGTACTTATGTAGGTTCAATGCCAGGTAAAATTATTAAAGCTATCCAAAGAGCTGGTGTATCTAACCCACTTATTTTATTGGATGAAATTGACAAAATGTCATCTGATTATAAAGGTGATCCAGCTAGTGCAATGCTTGAAGTATTAGATCCTGAACAAAACACAAAATTCCAAGATAACTATCTTGAACATGAATATGACTTATCAAAAGTTATGTTTATTGCAACTGCTAACTACTACGAAAATATTCCTGCTCCTTTAATTGACCGTGTGGAAATTATCGAATTAAACTCATATACAATTAATGAAAAAATTAAAATTGCACGTGAACACTTAATTGATTCTACAATTCAACAAGCAGGTCTTAAAAAAGAACAATTCAATATTGATGATAAAACACTAGAATTTATTATCAAACACTATACAGCTGAAGCTGGTGTTCGTGGACTTAAGAGAGATTTAGATAAAATTGCTCGTAAAATAGTTACAAGAATTGTTGAAGGTGAAAAAATTGCTAAATTTACAATAACAAAAGATATTGTTACTGAAATGTTAGGTACTCCTAAATTTACAGATCACCAAGATACGAAAGAACCTCAAGTTGGAGTTGTTAACGGTTTAGCTTACACTTCAATAGGTGGAACAACATTGCAAATCGAAGTTTCAAGCTTCAAGAGTAAAAATAGCGGTATCAAATTAACCGGTTCGCTTAAAGATGTTATGAAGGAATCAGCCAATATTGCTCTTTCATATGTAAGAGCTAATGCTGAAAAATTTGATATTAAAGATTTTGCTTTTGATGAAAGAGAAATTCATATTCACGTTCCAGAAGGAGCTGTTCCTAAAGATGGGCCTAGTGCTGGTGTAACATTTACAACTGCTTTAGTTAGTGCTTTAAGTAATAAACCTGTACCTCAAGATATTGCAATGACAGGTGAAATTACTTTAAGAGGTCGTGTTTTTGAAATTGGTGGATTAAAAGAAAAATCATTTGCTGCATATAAAAAAGGAATCAAAACTGTATTTATTCCAAAAGACAATGAAAAGAATTTAGCAGATATTCCTGAAGAAGTTAAAAAAGTAATTACCTTTATTCCAGTTGAAACTTATGAAGAAATTTATAATCACATTTTTAAAGATCAAAAGCCTACAACTGATAAAAGCGATTCGACTAAAAAGTCTATAAAACAGGCAAATAAGAAATAATTTAAATGACTTATATTCGTTGAATATAAGTTTTTTTAATACCCTCATATTTTTTAAAAAGTAACAACTAATGTATAATATTTAAGATGAAAGAAATGCAAATTATAAGTTATGTTTTATTATCAATAACTTTATTATTAGCTATTATCGGAACAATTTTTGGAACAAATTTTTATTGAAAAACTCCTAAGCTTAATAAAGTTTTTAGCTATAGAACAAAATTATCATTAAAAAATCATGAATACTTTTATTATGCAAATCACACTTTTGGAAGAGGTTTGTTTTCTCAAAGTATAGTCATTTTAATATTGTCTTTTATTTCTTGCAGCATTGTTTTTTCACTTAAAGATTATCATTATTTATTCATAATAATTTTTGCAATTTGATTTTTGAGTTTCTTTTCAATCATTATTTACATTGAATTAAAACTTAAAAAAAGAGACAAAATAATTACAGATTTATAAAAAATCCATAAAAAATAGAAGCATGTAACAATGCTTCTAATATTTATTTTTAATGCGTTCGTAATTCTTTTTGTTTTTGTTGTAATAAGCTTCTAAAAGATCTTTTTGACTGTAACCTAATAATTTAGCAACCCCTAAAGTTAAAGCTAATATTTCTCCAATAACATATTTATTGATGTTATTTGTTGCTGAAGAAATTGTTTGGAATAATAAGCAAATTTGTTTATTAACATCCTTACTCATTATTAATGGTGTGATGTTAGGATCAACTTCATATTGATAAGCAAAAGAGCCAACAAAGTGCAATATATCAGCAAATTCTTCTAAAGCTTTGTCTTTATCAATTTGTTTTTTTGCTTTTCAATATTTGAATGTTTGGACTTCATTTGCAAATTCTGCCGTTTCAACAAGTAAAGCTAATAATTTCATGTAGTGAAATTCTTTAATTTTGCTTTTTGAAGTCTTTGCATCAATATCATTACGAGCTGCAATAGCTTTGTCAAGATCTTTTTGCATTGTAAATATTTTTGTTAAATTCATGTTTCAATTATACAACAAGTTTAAAAGCGCAATAAAAAAGCTAAATTATTATTTAGCTAAATATTTGCTTCCTTTAATATATCTAAAGAGTGAGTTGCTTCTTTTGATAAAGTATCTTTGCTATTTAAATATGAGTTAAGTATTTCTTTACTTGCTTCAAAATTAAAAGCTGATAATAATGTTAAAAATTTCCAAACAAAAATTTTATTATCAATATTTTGACCATAAACATCAATTAGCTCATTTAAAAGTTTTTCAGTAGCTAATTCTTTTTTATTAAAACAAATGTAACCAATTGCATCAATAACTTCTTGTTTTTGTTCAATTGTTCCTTGCTTAAGAGTTTCAAGTAAAAGAGGAAAAATTGAAGTATGCATTTTAGCTAAAGATTTGGCAATAATGTCTCTTGGTAGTGGATAAGAACTTTTATTACTAATATATTTAGGCAATTCTTTAATTTGGTTATTACCAATTTTGCTCAAATATTCAATCATTTTATTAGCTGTAAAAGCATTACCTGTTTCTAAAGTTTCACAAATAGCGATTTTAGTATAAAGTTTGTCTTCTTTTTCTAATCGTTTTAATAATGCATCTACACTTTCTTCATCCATAGGAGATAAATTATAAGCCGCAGAAGATCTAACTCATGCATATTCATGTTGCAATAATTCTAGTAATTCTTTTTTGCTTTTGTTTTCAAATTCTGCAATATCTTCTTGATCAACATAACCTCTTGATAATCATTGTTTTTTATTTTCTACTGGTTTCATAAGTATCCTTTTGATATAAAAATGAGCCTTAATTTGGCTCATATATTATATAATTTTATTATTATTTTTCTTCTTGTTTTTCTACTTCTTTTTTAACTACAATAACTTCATCTTTGAAGAATTGTACATTTGCTTTTTTTCTAAGTAGTATAGCAGCAACAAAGGAAACAATTCAAATAAAGCAACCAATTATAAATAAAATCATAAATGTCTTATCATTTCATTCTGAAGTGTAAATTGCTAAGAAAATTGAAAATACTGTTATTGTAACTCAAACTGCACCTAGTGCTAATCCAACAATAAGTGTAAATAATGAAAAAGTTAGTGCAGATGATCGAGTATAACCATTAGTCGCAATAACAATACTTTGAATAATAGCTAAAAGAATTAAAAATCCAATAAAGCTACATGCAATAATACTGCAAATAACTATTCATTTAGTATAGCTTAATTCTTTTGCTTCCAATTTTTCATTGTTTACTTTGTTTAAAGATTCTGTTTTGTTTTCTGTTGACATAGATCTCCTTTTCATTTAAACTCAAAAACATCTATTTTCCGATGCAATTTAATTTTATATAAATTTAAACAAAATAAAAAAATTTTTTAAAAAAAGACACTCACATAAGAATGTCTTTAAAATTAATTCTATTCTGCTTTTGAGTCAATTGATTCATCTGATTTAAAATAATTGAAGTAGTTTTTATTTTCTTTTTGAGCATCAGAAATATAAATAAATGCTCCGATGAATGTTAATATTCCAACGAAGAATCCTATAATTAAGATTATCATTGATTTTTTATCTTCTAATTTGCTTGCTTGAATAGTTATAACAATATGTAATACAAATGAAGCAACGCTTATAGGAAGTATTGATAAAATTAATGCAGTAGGGATAATAATTTCTTTTCAGTTATTTTTAATATATTCATTTGTTTCTTCTCCACTTTTAAATATTTTAACTGCAACATCAATAAACATAAATATTGCAATTCCTGTTATAACAGCGCTTAAAAGTGATAAAACAATCAAAGCAATAGCTGAACTTTTAGGATTTTTTCTATGCATTTTTCTCCTTTAAAAATGTAATTTTATTACCTTAGTATTATAAAAAATAAATTTTAATTTTGAAGAAATATTTTGTTAAATGTTTCAATATGGTTCATTAATATGAACTAATTCAATTTTAACTCCAGGGAATTTTTCTTTGATTATTTTATATGCATCATCTGCGAAAACTTGTTCATCAAGGTGAGAAATTTCTAAAATATTGGCTTTGGTTTCTTTATAAACTATTCAGTCACTTCATTTAATATCGCTTGTAATAATAAGATCATAATTTAAATTATTTATAGCATTAACTTCTGAAATATATCCAGCCCCTGAAAGGAAAGCAATTTTACTAATTTCATTGTTTCATAGGTCTTTATGTAAATTTGTACGCATAGCTTTGAAGCCAAAAGTTGTTTTAATTTTTTTAACTAAATCATTAAAAGTAGTTTTGTAATTTACTAAATTAGGATAATTATGTTCATAATTTTGGCGATAATTTTCAAGTCCTAATTTTTTTATGATTTGATATGAAGTTCCATAGTATTGGTTGTCATAGTTAGTATGAAAAGCTAAAACATTAATTCTTTTTTCTTTGCATTTTTGATAAATTGATCTTTTGTATGGAGCTTTGATATCTTCATCATCTCAAGATGCTTCAAATTTAAAAGGGTGATGAATGATGATTAAATTAGCATCTATTTCTAAAGCTTTTTCGAAAACTTCTTTGGTTAAGTCAATAGCACAAACTATACTACGTAAAGGTTCTGATAAATTGAATTTAATAGAAAAACCACTTGGGTCTCATTCTTCTTTAAGATCAAGTGGTCACTTTTCTAATAAATATTTTGTTACTTTTCTAATTTGCATTATTTAATGAAATCTTTCAAGTGACGTCCTTTACGTGTGCTACTTTTAAGTTTTCTTAAAATTTTGTTTTCAATTTGACGAATTCTTTCTTTAGAAACATTTCTTTCTTCTGCTAATTTTTCAAGTGAGTGAATTGGGTATTTATTTCCATTTTCGTCAACTCCAACTCCGAAACGTTTGCAGATTAAAATTCTTTCATCATCATCAAGTGATTTTTCAAGAATTGAATTTAGTGTTTCCACTAATTCTTCTTGTGAAGCATAATCAACTGGATTAACAACACTTTCATCTTTAACAAAGTCACTAAATGATGAATCATTTTCTTTACCAATTTGTTTGTCTAAAGAAATAGGGTCAATGTTGATTTTTCTAATGTATCTAACTTTTTCAGCGTCATAGCCATTGCCATATCTTTCAGCAATCATTTCATCAGTTGGTTCATAACCTAATTCTTGTTGCAATTCACGTTCAATTTTAGTGATTTTGTTAATAGTTTCAACCATGTGAACAGGTACTCTGATAGTTCTAGCTTGATCAGCTACAGCGCGAGTTATTGCTTGACGAATTCATCAAGTTGCATATGTTGAGAATTTAAAGCCTTTTGAAACGTTATATTTTGAAACTGCTTTTACGATACCTGAGTTACCTTCTGAGATTAAGTCAATAAAGCTAAGTCCTCTATTTTTGTATTTTTTAGCATTATTGATAACTAAACGTAAATTACGTTTGATAAGTTTATCCCTAGCACGTTTTCCTCTAAAGCCACCTTTTTCCATTTCTTGAGCAAGTTTCTTTTCTTCTTCAATGGTTAAAAGTTTTCCATATTTACCAATTCAACGCATGTATCATTTAACAATATCATTAGTTTCGGTAAGTTTGTTGGTTAAATCTTTATCTTTAATTACTTTGTTATCATTAAGGTTAATTTCTTCATTTTCATAAGATTCAAGTTCAAGTGAATCTTCATCTTCATTATTTTTACTTTTTTTCGATTTTTTCTTACTGCTTAATTCTTCTTCGTCTTCATCTTCGTCATCTTCATCATAACTTGAATCAACATAGTCATCTTCAATAATGTATTCTTGACTTTCATCATCGTCACCATATGAACCAACTAAATTGTAGTTATCATCTTCATCTAAATCTTCGTCATATAAATCACTTTTTTTGTTTTTAACATCCATTTCATCGGTGTTTTCGTCTTTTTTGCTTTTTTCTTCGAATTCTAATTCTGAGTCTAATTCATCTAAACTAACATCGTCTAAATCGCCCTCGTCGCTTTCATCAAGAATTAAGCCTGATTCAAAAAGTTGGCCTAAAACTTCGTCCATTGATTCATCATCAATTTCAACTTTAATTGAATCTAAATAATCATAAACTTCAGACTGACTTATTTGTTTTTTCTTTAAGCTTTTTGCATGTTTTTTAATCATTGAGATTATTGATTTAATTTCATTTGCTTTATTCATTTTCTTCCTTTCTTTTTTGCATTTCTCTTAATTTTTCTTTGTGTTCATCATACATTTTTTTGATACTGCTGATTTGTTTCAAAACTTCAGGATTGCTTGTGTATTTATTATAAGCATTATCTGATTCCTTTAATGCTCGGCGATAAACATCTTTAAAGTTTTGTTTAATAATTTTATCCTCTTTGTATTTTTTTGAAAATTGATTTAAATCGATATCAACATTTTCCTGTTCTAAGTCTTTGATATATTCCTTTAATTTAGCAACCTTAATACTTTTACTTTCTCTAAATGTTTGATAAAGTTCTTTTTTTAAATCTTCATTATTTAATTTACCGAAAAACATTAAGTCTTTGTGTGTATCTTCTCTTTCTTTAAATAGCTTAATTAATTCAGTGTGATTAAGTAATAAATAGAAAAGATTGTTAATTCAATAATCGCTTAATGGCACTTCGTTATTTAAAGGGTGATTAAAATTATTGACATTATTGCTGTATTCAACTACTTGATTATAATTTTGTTCATCATAAGCATAAGGGGGAATATTGTTTTCTAATAATTGATATTCACCTGAGTCTTCTGGAAATTGTTGTGGTGCATAAATTTCTTGAGAATCATAAGGATCAGGCAACTTAAAGTTGTGTTTTACTTGAATGCTAAAACCATAATCGGTTTGAATTTTGTTTTTGTAGAATTCTTGAACATTAATTTCTTGGTGTTCAAGGAAGTTAACAAAGTCATTGCAAAAAGCTTTAACATTTTTGATTTCATTGTTTGGCGAAACACTGAGATTATATTTCTTGCTTAAATTTTCATAAATAAAATCTAATGCAGCTGGAGCTTCTTTAATTAACTTGTGAAGAATATCGGCACCATAACTATTAAATAATTCATCGGGGTCATATTTAGTTGTGTTACGTACTATGTAAGTGTCAATACGATGGTCTAATAAAAACTTAGCACTTTTTAGTGAAGCTTTTTGACCAGCATCATCACCATCTAAAAAGATGACTACTTTTTTGTTTTTTAGTAATCGGAAATGATTATTGGTTAAAGCTGTACCCATTAAACCAACAGCATTATAGATTTCAGCTTTAAAAAGTGCAATAACATCAAAAAATCCTTCAGTAATTATGATTGAATTGTCTTCGCTTTTACTATTAGCTTGTCAATAGTTATATAAAAGAGAATTCTTTTTGAAAAGTTTAGTTTCATTACTATTGATATATTTAGGTTTAGTATCATTGTCTAAAGCACGAGCACTAAATCCAACTATTTCACCTTGTTCATCTTTAATAGCAAAAGTAATTCTATTCTTAAATGCGACACTTTTGTTATGACTATTAATTAAACTTGCTTTGACTAATAAGTCAGGAATATTTTCTAACTCATTTGCAAAAATTGAATTGAAACTTTCTTCTTCACTATAACCAATATCAAAAGCCTGAATGATTTCACTAGTTAATGCTCTTTTATGAATAAAATCTTTTAATTCTTTGTTTTTTCTTTTTATTAATTCTAGTTTAAAAAGTGAATTAGCTTTGTTGTTGATTTCAATTATTTTTAAGTCTTCTTCATTGTAACGACTTATTGATTTTTGGAATGAACTTAAGTCAACATTGATATTACTACGATTAGCTAAATATTCTAGTGATTCTTGGAAAGTTCATTTTTTGTATTTCATTAAAAATGTTAAAACATTACCACCAACTTGGCAGACAAAGCATTTAAATATTTGCTTTTTAGGGTCGACATTCATTGATGGATTTGTGTCTTGGTGAAAAGGACAAATGCAAACAAAACTACGACCTTTTTTGCTTAAAGTAACAAATGAGTTGATAACATCAACAATATCATTACTTGCAATAATTGAGTCAACTATTTCATGGTTATTATTACTCATTTGTTACCTCCGTTTTTTTCTAAAACTATTGTTTTATAGTTCTTTTAGTAAATTATTAATTAGTACAGTTTTTTGTTTCATACTGTCTCTGTCTCTAATTGTTACAGTATTTTTTTCAAGTGAATCAAAGTCTATTGTAACACAATAAGGTGTACCAATAGCATCTTGACGACGGTATCTTTTGCCTATTGATCCAGTTTCATCATAAGTTACAGATAAGCCTGCTTTTAGTAATTTTTCATAAACTTCTGTTGCTTTATCATTTAGTTTTTTAACTAAAGGTAAAACTGCAACTTGATAAGGAGCTAAATTTTTGTGTAGATGTAAAACAACTCTTGAGTCATCTTTTTCAAGTTTTTCTTCGTCATATGAATCAATTAAAATTGCTAACATTAAACGGTCAAGTCCTATTGAAGGTTCAATAACAAAAGGAACAAATTTTTCGTTTGTTACAGGGTCAACATATTCTAAGTTTTCACCAGTAGCTTTCATATGAGCATTTAAATCATAGTCAGTACGATTAGCTATTCCTAAAAGTTCACCTCAGCCAAAAGGGAAAAGATATTCAATGTCACTTGTGCCTGAAGAATAATGACTTAGTTCTTCTTTTTCATGTTCTCTGATACGAACATTTTCTTTGCTTAAGCCTAAATATTCAGCAAATTTTTCAGCTTTTTTAATGTAGTATTTGTAAAGTTTGTTTGCTTCTTTTGGATGGCAAAATACTTCAAGTTCCATTTGTTCAAATTCACGAGTACGGAAAATAAAGTTACCTGGTGTTACTTCATTTCTGAAACTTTTTCCAACTTGCCCTATGCCCATAGGTAATTTTGAACGCATGCTTCTTTGTACGTTTTTAAAGTTAACAAAAATACCTTGAGCAGTTTCAGGACGTAAGTAAATTTTGTTTTTGGTTTCTTCTGTAACTCCTTGTTTGGTTTCAAACATTAAGTTAAATTTTCTAATATCGCTTCAGTTAGTTTTAGCACCATCGTATTCTTTAAGATTCTTTTCAAGGAATTTTTTGATTTCATCATTAGTCATTTTTTCTGGAATAATGTTAGGATCAATCTCTTGAACTAATTTGTCAGCACGATATCTTTTGCCATTAACTTTGTTTTCAATTAAAGGATCATTAAAGTTAATAACATGACCTGAAGTTACTCAAACTTGAGGATTCATTAAAATTTTTGAATCTAGTAAATGGTTGTTTTTTTCTTTTTTAATAAATTCGCTTTTTCAAGCAGCTTCAATATTATCCTTTAAAAGTGAACCAAGAGGACCATAGTCTCAAGTATTAGCTAAACCACCATAAATTTCACTACCTTGATAAACAAATCCACTAGCTTTTAAATGATTTATTAAAGTAGTCATATTTTTTTCATTATTCTTCATAAATATCTCTCCTTTTATTAATTGAATAATAGTAAATAATTACAAAAAATGCATCTAAGTGCATTTCTTGATAATTAAATTAAGCACCAAAATTAAAGAATGTATAACCACCTGCTAATTGAATAATAGCAAGAATATCTAAGGTTAAAAGACCTGCAAGAATTAGTACTAATAATAAGATTAAGAAGTAGAATCCTGCTGAACGTTTTTTCTTAGTTGAAACATTGTTTGATGATTCTTCTGATTCTAGAGCAACTGGTTCATTACCTCTAATTCCTGTGTAGAAATTATCTTCAGGTTGAGCTTCTTTTTCTTCAATTACTTGAATAGGTTTTTCTTCAACAACTCTAACAGCAAAGTCTCTTGTTTTAGGTTTTTCAGCTTTTTTATGGTCAATTAAGACAAAATCATTGTCTTCAACTTTTTCTTTTTTAGCTTCTTCAGGAGCTAAAACAATAAGCGGTTCTTCTTTAATTGTTGGTTGTTTTTCTTCAACTGGTTTAACTTCTTCAACTACAGCTTCAGCTTCAACAACTGATTCATCTTTAACTTCTTCAACTACTGGAGGCACTTCAGTAACTATAGTTTCAGTTTTAGGTTGTTTTTTAGTTTTAGAAGATTTTTCAGCAGCTAAAACAGCTTCTTCCATTTCAACTGCATCTCTTTCTGCAGATTGAATTGTTATTGTTTTAGTAGCATCTGATTCATTAATAACTGTTACAGCAAATTTGTTTCTAATTGCTAAATATGGAATTGCATATTCATATTCTTCATAAGGAACAAATCCAACATGATAAAGATCAACTAAAACATATGAAGTAGCTCATGAGATTTCTTGTTTTTGCTTAGTTTCTTTATTTAGGAAATAACCGCGGTTAGCTGGTGTATCTTCTAATTTAGGATCTAAGACTAATAAACTAAGTAATGATTCAAAAGTTGTACTTAATTCAGCATGCATTTTTTGAACTAAATCAGCATGTGGGAAGTTAGGATTTGCTTCAACTTCTAATAATTGTTTATTGAAGTTAGCATAGTTTTTCTTAATATTATTGTAATCAGCTTCACTAATATTTTGTTTTGGAGTTGTTTCAATAATATTTTTAATTTTTTCTGTGTATAAAGCAACACCACCTACATAGTCATCAAAACTTAATGTGATGTAGTTCATGTATGTTACACCTTCAAGTGGCTTGTAGTTTTGTCTTAAAAGTTCCATTTTACGAGCCATTTCATTGAAGTTCATATCTTTTTTTGCTAATTCTTGTTTAAGAAGTTCAAGTTCTTTGTCTGCTTCAGCATTGTTTTCATGAATTTGAGTTAAAAGAATATTAATTTGTTCTTGGAATTGTGCTCTAATAGCATCAACGTCAACAACATCTGATTTCTTTTTCTTTTTAGCAATTTCTAATTCAGGTGGAAAGTAGGTTGCTGGATCAGAAATTAATACAAAATCAAGATTTCTTAATCTTCTTTTTGCATCATAATCATCGTGTTGGAATGTTGAAGGATTAATTCCAATTTGACGACAGTTACCTTCATATGTACCGCCACCATCAAAACCAGCAGTTTTAATGTAGTAGCTTCATTGTCCATCTTCACAATCACAAGTTAATTGACCTTCAAAAATCTTTTGATCATTTTGATATCAAACAGCTGTTTCATACTTAAGTAACATGTATCATTCAAGAGCTTCTTGTCTTGTTTTAAATTTTGCTAATCCAATTTTGATTTTTGGATGTTTTAGTAATCAAGGATAAATTGGGTCCTTTTTAACTTCATATGTACAGTTAAGTCTTTTCATATTGATTCCTCGCTTATTTTTTACTAATTAGCCAATAAATAGTGTATTTATTGAAAATTTTTTTTACTATTTTTGGCCTCAAACAATAGTATTTTATTATTGTATTAATATTATAAATTAATATTTAATTTTATAATGCAATTAGTTCAAAAAAATTAGCTAAAAATAAATATTTTTTATTTTTGACCAAAAAAATTGCTAAAAAATCAAATATTTTTGCATTTTTTCTTATTTTTTTCCATTTAATTATTAATATAAACATAAAGTTTAAGTATAATAAAAACACCATGAATCATAAAGGCGATATTTTATTCGGAAAAATAAAAAGTGTAAGTAGTGAAGGACTTACAATTTTATGCAATAATGATTATTCTTATTTTATTCCTCGTGATTTGATAACTGACTGAAACAAAATTAACTTGCGTCATGAATTTAAGATCGGCGAAAAAATTAATTTTATAGTTGAAAAAGTTGATTATCACAGTCATAGTGGTATTGGTAATTTTAAAATTAATCATGCCACCTTTTCACGCAGTCCATTTAAAGAAAATTTAAGCAATACAAAACATGGTTTTGAAAACTTGAAAAACCAAATTGATCAAGAAATCAAAAATTATCATAAAGAAGGTAATAATGGCAACAATTAATTTGAAAGCTTATAATTATTCACCTGATTTTAAAAATGAAAGTTTGATTAATCGTGGCAATAACTTGATTAAGGATGTTAAATTAAAAAATATCGAGGGTTTTGAACATTTTGGTTTTCATGAATTAGCTTTAAATTTTAGTAATTTTAATGTTGATGAATTAAACAATTTTGTACGCTTAATAAAAAAGCAAGAAATTAAAGAAATTTTGATTATGACAACAAGCAAAATAATTGATAATTATCAAGCTGGAAATGACTTTTTATTTAAGTATGATTTATTAAAAAAATACAAGTTAAAATTTAATTTTATTAATGTTGATAATTATGAGCAAGATTACTATGCTAAGTTTGAAGAGTATAAGAAAAAATTTGAACAAAAAGGCATTGCTTTAATGTTTGGATCATTTACTAAATTTAGTGAAGAAACAATTAATTTTATTAAATTATTAATGAATCATTTTCAATTAAATCAAGGTTATTATCGAGCACTTGAAAGAGTTTTTTTAATCTCAAAAGAATCATTAGAAAAACAATTGGAATTTTTAAGTATTCCCCTTCAAAACAGACTTATTAGTCCAAATATCCTCTCAAATAATTTTGCTTTTTTTAGTGAAATTAATATGTTACTTTTGTTGCTTCAAGGAGCTAATATTAATGACTTATCTGAAGGCTATTCTTCAGGTTGCACAAACTGATTAAGTAGTAACTTAGAACAAAACAAAGCATTCCAATATGCCTACATTAGGTATAGCTTAAAAGGTGATCGAAAAAATGATCTTTTAATTGGAACTGATGAAAGATTTAAAGGTATTTTGAAGCTTTATAGCACAATAAATAATAGTCAATATTTAAAGTCAAATAATGTGACAATGTATGCAATTTTTAGTCAAGATATTTATACTTATGGACAATATTTAATTGATAATAATCGCAACTTTTTTACCTCAATTTTTAGCATTGAAAATGAAAAAATTGACTTTAGAATTAGTGATGAAATTAATAATACTCAAGATGGTTTAGAGAACCATAAAGATAACCGCATAAGCAGCTATTACAAAGTAGCAAAAAAAGGTTATTTGACAACACTTTCAAATGTTGCATCATTACCAGTTTTTGATATTTCATTGGAAAGTTCAAATGAATTAAATTTAGGATACTTAGTTGGATTTCTATATTGAAGTTATATTTTTGAATGTTACTTAAATAAATTGAATCCTTTTGATATATAAAAATTGTAATAATTTCCATATTTTTTAATTAAAAGAAGATAGTTAACAAAAGAAAATATTAAAAATGTAGAATAATTAAAGAAATTATTAAGTTAAGGAGAAAAAAATATGAAAGCAGTTAAAGTTAATTTACATAATTGTGTTGATGAAAAAACATTACTTTCTTATCAAGACAAAGTTAAACAAATTCATGATTCAATAATGAACAAAACTATTGCAGAAAAAGATTGATTAGGTTGATTAGAATTACCTAACAAAGGCAAAAGTGAAGAAGTTAAAAGAATGAAAAAGATAGCTGAACTATGAGAAAGCAAAAACATTGAAGTAGTTGTAGTTATTGGTATTGGTGGGTCATATTTAGGAGCTAAAAGTGGCTATGAATTTATGTATGGACCTTACAGCATTAACAAACCAAAAATGGAATTATTATTTGCTGGAAATAGCATTAGTAGTGAAAGCTTAGTAGCAGCATTAAAATATGTTGAAAACAAAAAGTTTGCAATTAATGTTATTTCAAAAAGTGGAACAACTTTAGAACCTTCTATTGCTTTTAGAGAATTTAGAAAATTATTAGAAGCAAAAGTTGGAGCAGTAGAAGCTAAGAATTTAATTGTGGCTACTACTGATGCTAAAAAAGGCGTTTTATACTCATTAGCAACTCAAAAAGGATATGAAAAATTAATTATTCCTGATGATGTTGGTGGACGTTTTAGTGTTTTAAGTCCAGTTGGTTTATTCCCATTCATTTGTGCGGGAATTGATATTGAACAAATTCTTAAAGGTGCTCAAGAAGCTAATAAAGATGCAAATGATTTAAGTTTAAAAACAAATGATGCTTATCGTTATGCGGTTGCAAGACATGTTTTAAGTAAAAATTACACCATGGAAATGATGATTTCTTATGAGCCAAAAATGCAATATTTTGCAGAATGATGAAAACAATTATTTGCTGAAAGTGAAGGGAAAGATGGCAAAGGATTATTCCCAACAAGCGGAATTTTTTCAACAGACTTGCACTCACTTGGTCAAATGATTCAAGATGGAAATAAAGTATTATTTGAAACAATTTTAGTTTTAGATAAACCAAAATACGATATTACTTTTAAAGCTGATAATGAAGATTTAGATAAACTTGTTTATTTAGATAAAAACAAATTGCATAATGTTAACATGACAGCTTTTACAGCAACTTTAGATGCTCACATGAACACAGGTAAAGTTCCAAACATTGTAATGAATTTTGCAAAATTTGATGAATACACATTAGGTTACTTATTCCAATTCTTTGAAAGAGCTTTAGCTATTAGTGCTTATCTTTTAGGTGTTAATCCATTCAACCAACCAGGTGTTGAAGTTTATAAAGCTAATATGTTTAAAATTCTTAAATAATAAAAATTTAAATTATAAGTCTTGTTAAAAGGCTTATTTTTATTCTAAATTTTATTCAAAAAGCCTAAAATGGTGGAACACATTCCACTGAATTTGGTGGAATGTGTTCCACCATTTTAGGAATTTTTAATAGACTCTAATAACTTTTAATATATAATATTAATAAATAAAAAGAAATATTTATGAAATATAAAAAAGAAATGTATTATGAAAGATTATTAGATCCAATAATAAGAAAATATTTAAAAGTTTTTGGAGCAATTTGCATTCAAGGACCTAAATGATGTGGCAAAACATGAACATCACAATTTCACTCAAGCAGTTCATTTTATGTTGGAGATTCTAGAAATAATTTTGAAAATAGACATTTAGCAAAAATAAATCCAACTAAAATTTTAGAAGGAAAAACACCAAGACTAATTGACGAATGACAAGAATATCCACCTATTTGAGATGTAGTTAAATTCGAAGTAGATAACCGTGGACTTAAAGGTCAATTCATTTTGACTGGTTCTTCTACTCCTGAACATAAAGGCCTATTTCATAGTGGTATTGGTCGAATTGCTACATTACAATTGAGACCTATGAGCTTATTTGAATCTAAAGATTCTTTAGGACAAATTTCATTAAAAGATTTAATAAATGACAATTTTGAAAATACAACTTATGAAGGTATTGAATTAAATAAAATTATTAATTTAATTATTAGAGGTGGTTGACCTGACAATATTGAAGTTGATGATAAATACTCTTATTTAGCGGCAAGTAATTATGTAAACACTTTTTTAACTGAAGATTTAGATAAAATTAGTGATACTAAATTTGATAAAAATAAATTACAAAAATTATTAAAATCATTAGCTCGAAATAATGCGATAACAGTTAGCATTAATAAACTAATTGATGATATTAAAACAAATGAAAATGAAACAATTGATAAAAAAACTATTTATGCATATTTAGATATTTTAAATAGATCTTTTATTGTTGATAATTTGCAACCATTTAGTTTGAATATTAGATCCAATTTAAGAATTAAACAAAATGAAAAAAGACTTTTTGTCGATCCTTCTTTAGCAGCTGCGTTATTGAATGTTAATAATGAGAGTTTGCAAAATGATTTAAATACTTTGGGTTTTTTATTTGAAAACCTTGTCTTAAGAGATTTGAAAGTTTATGCTCAAAGTAATGAGTGAAATATTTATCATTATCAAGATTATAATGATAATGAATTAGATGCTGTTATTGAATTAAAAAATGGCGAATGAGTTGCAATTGAAATTAAATTAGGCTGAGAACAAGTTGATAAAGCTTCTGAAAAATTAGTTAGATTTTCTAACAAATTAGAAAAACAAAAGGCAACAAAACCTAAAAAATTAATCGTAATTACAGGAACAGGGAAAAGTGCTTATTTAAGAGAAGATAATGTTTATGTTGTGCCTATTGGATTGTTAAAAAATTAAGAAAAAAGAATATTGCTAATTAAAGCAATATTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGAGGTTATTTTGCTTTATATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGTAAAATGCGGACTGTTAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTGATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTCATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGCGAAAAAAGAGAGAAACAAAGCGCTGCTAGAAGACTGGTCGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAATGAAAGAAAAGAATTTGGCCATTGGGAAATAGACCTTATTGTAGGTAAGAGCAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTTTAGATATGGATTTTTGGTAAAAATTCCTAATAAAAATCCACGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTTAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGAAGTTCAAATGAAAATTTCAATGATTTAGTTAGAAGATTTTTTTAAGAAAAAGACTAATTTTGATGATATTACTGAAGAAGAAATTTTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGTTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGCAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGAAGGGTTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATTGACATGCAATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAATATTGCTAATTAAAGCAATATTCTATTTTTAATTAACAACATTTGCATTCTTTGCGGCATTTTTCTTTGCAACATGATGAACAACATGAATTTTTGCAGCAATCTTTTGTTGTACATTTAGTTGCACAGTCTTTTACTTCACAATTTTTTGTGCAACAATTTTTCTTTGAACAAGCCATAATTTAACCTCCAATGCATATATATTAAATTATATTCTTTTTTTAATATATAATTAAAACATTATGCCAGAATTACCAGAAGTTAGAAGTGTGGTCAAAGATTTAAGACCTAAAGTTGTTAATAGAAAAATAGTTAAAATTGATATCTTACATCCTAAATTAATTAAAGAGGTAAGTGTTGAAGAATTCAAGAATTTTTTAATTAATGAAACATTTTTAGATGTCAATAATTTAGCTAAACATATTATTTTTTCTTTAACAAATAATAAATATTTATTAAGCCACTTGAGAATGAGTGGAAAGTATTTTACACATTATAAATATCGTCCAGCAACAAAACATGATTACTTAATTTTTCATCTTGATGATAATAGTTGTATTTATTATAATGACTCACGTCAATTTGGCACTTTTCATATAAAAACTAAGGGCACTTTATATACAACTAAACCTTTAGATAAAGTTGCAAAAATTCCTTCAGAAACAAATATTAAAGAGTTATTTAATAAAATTAAAAATAAGAATATACCTATAAAACAGCTTCTTTTGGATCAAAGCTTTGTTTCAGGTATTGGAAATATTTATGCTAATGAAACTTTATTTGCCACTCAAATTAATCCTTTGACGCCTTCAAAAAATATAACTTTTGAACAGTTAGAAAAAATTATTAAAGCAGCTGCAAAAATTATGGATCAAGCTACTGAATTAGGTGGTTCAAGCATTGACACATATACTTCAGTTGATGGAGTTAAAGGTCAATTTCAAGATTTTTTACAAGTTCATGGACACTTTAATGATGTATGTAAAAGATGTAAAAAAGCTAAAATTAATAAAATTTTTATTAACAAAAGAGGGACTTATTATTGTCCAAATTATCAAAAATAAAAAGCACTTCTCAATAAATGAGAAGTTTTATTTTAATCTTTTAGTAGACTAATTGGAATAATAAGTATTCCATCTGTTATTTCATATGCTCTATCTTCTGCTGTTAAAATAATCATAAGATCCGGTTCTCTTTGTCCATTTTCTTTTATCAGCTGTTTTACTTTTAATAAATTATTTTTCGCTTTTTCAATTTGATTTCCACCTAATTTACATTCTATCAATGCATATCTGCCATCATTTAAGTGCAAAACAAAATCAATTTCAAGACCATATCTATCGTGATAATATGATAATTCTCCATTTAAACTTGATGAATATATTTTTAAATCTCTAGAACACAAATTTTCAAATACAAAACCATATGTTCGTAAATCCTTTATTAAATCTATATATGAAATACCTAAAGAACTTGTTGCTAAAGAAGGATCTACAAAAAGTCTCTTGTTTGTACTTCTAATTGCTGAAGCTGACCTAATTTTAGGGTTTCATGCAGTTATATTATCAATAACATTTAAATCTTCAAGTTTTGTAGTGTAATCATAAAAAGTTGTTTTTGAACCTTCATCTTTAAAACTACAATCTTTTATTAAAGTTTTTGAAGTAGCTAAAGTAGCAACATTTCTTGCATAACTTTTCAATATTCTTCTAGTAATTTCAGGGCTTCTTTTTATGTTATCAATTTTACTTATATCTACATTGCAAATTTGATTGACATAATCTTTAGGAATTTCGATTTGATTTTCATATTTTTTTAAATTTAGAGTACTTGGCCATCCTCCTCTGCAAATTGCTTTTGCTAAATCTTCAAAAGATAAATTTGATTTTAAACCATTTACTTTAATTTTTTTATCAAATAAATCTTGTAAAGATATTTTTCCACTAGATTCTCCAGTTTCATATAAACTCATTGGATACATTTTCATTGTATGTATTCTACCAGTACCAGTATGCATAGTATTTAATTTATTGTGTGTACTTGAGCCTGTTAAAATAAATAACCCCTTTTTTCCCATTGCTGAATCAACATAAGTTCTTACGGCATCTCACATAATTGGAAAGTCTTGTCATTCATCTATTAATAATGGTTTTTCTCCTTCTAAAAGTAATGAAGGTTTATATTCAATTGTTTCTTTGTAAGTTTTTAAATTGTCTGGATCTTGTAATTTAATTAATGTTTTTGAAAATTTTTGTGCAGTAGTTGTTTTACCACATTGTTTAATTCCTTCTATGTTTATAGCACCAAAAATTTCAAGATTTTTTTTTATTTGTTTTTCTATTATTCTTCTTTTATATTTCATAGCAACCTCGTAAACATTATAACAAAAAAATAATTTGCCGAACACTTTTGTGGTAATTTGCTGAACACTTTTGTGGTAATTTGCTGAACACTTTTGTGGTAATTTGCCGAACACTTTTGTGATAAATAATATAAAAAGAACAAAAAAATGAGCATTTAATTACTCGCTTTTTTGTTCTTGAAGATTATTTTCTGGTTCTTTTTCTAAAACTCTAATAACTTCATGAGAGTCATTAAAGTTGCAATTGCTCCATAAATCCTCTACATATTTTGATTGATAATTTTCTAAATCAAATTCTTCTAAAACATATCATTTTTCAATAGCTTTTATCAAATAATAATTAATTATTGGGGCTACTATTACTAATACTCAAAATCCTAAATATAAGCCTAATTCAACTCATTTACTAATAGAGATGTCTTTACCATAGAAAACACTGCCTAGTGCTTTATCATTATTTACTATTCCGTCAATTGTTGCGCCTATATAAGCAACAATTGCCAATAATGATCCTACAATTGCAAAAATTGAAAAGATTCAGAATATAACTGTATTCATCTTTTTAATATTCAATGTTTTTTTGTGTAAATACAAAACAATAATAACAGCATAAAAATTGAAGAAGATAAATGTTGGCAAATTGCTTTAACCATCTAATATGTTATCGCCACCTAAAACTCAGATGCAAATTGAAATAATTAGTCAAATAGCACTATAAATAAGACTTATAATTAAAATCATCATTCTTTTAAGATTCATTTTGTTTCTTATTTTAGTAGTATTAATTATTATTTTTAAATTAATAGCATTATTAATATTATGTGCATATGAATTGGTAAAATCATTTAATACTCCAACAACAGCAATAAATAAGAATAGACTAAAAATAATGCTTAAACTTTGCACTAAATGTTGATTTGTTCTTTGATCAATTGTATTTTTAATTATTTGTTCAACAACTCCACTATTATGTAAAATTGCAAAAATAGCAATTAAAGAATAATTTATTATAACTGTAATCATTCCAACAATAGCAACAGTTGGTAAGTTATCTTTCTTTAAGTTATTAGCAGTAGATCCAACTGATAAAAATGTATAGTTAAAGCTGATGAAATAACAAAATTTTTTATTACCTAATTGTGTAGCTCAGTTGCTGATACCATTTGTTTTAGTATTTTTAAGTTGGTCAGTTTTGTTAAAACTTAAAGCATTATAAAGTGAAATTAAATTAACCCCGCTAAAATTCAAGTCATTAATCAACTTAAATTATCTTCACTAGTAGCTTTTTTATTAATTAATTTTTAAAAAATATCCCAAACCAACAACAGTTCCTATTAGCATAGTAAGTATAAAAAAACCAATTTTTTTAGCATTTTTATATGTGAACATTGCTTTAGCTCTTTTCTATGATTAATCAAAAATATTTTTTAGCAATAATAAAAATTATTTGTTTTTATTGTAAAATAAATTCATGAATATTTTTTATAGTATTTTGTTAAATTTAACTTTAATCTTAATTGGATATTTTTTACTTGGATCATTTAATACAAGCATTATATTATCCAAATGAAAGAAAAAAGATGATATTAGACTGCATAATTCAAAAAATGCGGGAGCGACTAATAGCTTAAGGACTTATGGTAAAAAGTTTGCAGCAACAGTTTTTGTTATTGATGTTTTAAAAACTTTTATTCCAACAATAGTTTTAGCAGCAATTTTAAATCATGCTTGTTTTGACTTTGCTCAAACTTATTACATGAGCCCACAATCTTTAGCTTTAGGTGTAATCATTGGACATATTTTTCCAGTTTACTTTAAATTTAAAGGTGGTAAAGGTGTAGCTTGTACAATTGGTTTAATTGCCACAATTAATATTGTCTTTTTATTAATGGCTTCTATTATCTTCTTTATAGTTGTCTTAACTTCTAAATATGTATCTTTAGCTTCAATATTAACAGCGGCAATATTAGTGCCCTTTGCTTTTATTCCTTGAATGACTAGTGGCATTTTAGGATATTGAACAAACTTTGTGTATGAATATCACAATTGCTTTGTAACAAGTTTTATCTATTTAATAGCTGCAATATTAATTATTGCAATGCATCATAGTAATATTACTCGTTTAATTAATCATACAGAAAGTAAATTAGGCCAAAAAAAAAGCGAAGATAATGGTATGAACCCCAAAAATTGGACTTTTTAATTATGTTTGGACTTATTCCTGTATTGGACAGGACTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTCCTAAAGCAGTTTTAAATTCTTTAAAACTATTAAATTTCTTTTCTTCTCCAAATCAAAATTCTCTTTTTATAACACTAAATAAACATTCAGTAGGACTATTGTCTAAACAATTTCCCTTTCTTGACATGCTTTGAGTTGTTTGTTTTTCTTTCAAATAATCAATATAATCTTGATGAGTGTATTGTCATCCTTGATCAGAATGTAATAATACATTTTTTAAGCTGTGGCCATTCTCTTCTACGTTTTCTAACATTTTACCAACCATTCTTAAATTAGGCGATTTAGAAATCGAATAACCTAAAATCTCTCTGTTTTCAAAATCAACTACAGGAGAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTGTTCCTAGTATTTTCAAATATTTATTATTTGAAAAATTTCTTTTGTATCTGAAGAAATCTTCTGTGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTGTGAGCATCACCTTTGAATGAAGAATATTTATTTTTTCTTCTTAGCGTTTTGCCGAAAATATTCATTGCTTTCATTAATTTTTGAACTTTTTTATGATTGATTATTATTCCTTTATTTCTTAAAGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAAAATAACCTTTTTTAAATTTTCATCTTTATCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAGTTGACTTTTTCAATTTAATTTTTTCTAATATTAATTTAATAAAAAATAGCTTTTTAAATTCTTGCCTTAGTTCAATTGTTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAGGCGCTCACTTTTTTTGCTATCGCATTCTCCAATTTGAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATTTTCTATTTTATTTTCAATATTGATTTTAAAATTTGTTGCCTTTTGAGCATCTAAATTTATTTTAGATTTTCTTCCTTTTTTCTTTTCCATAGCAAAAGAATTATAACCAAACTTTTTGTATTTTTCTATTACTGAATAGCATGCTGAATAAGAGTTTTCCATTATTTTAGATATTTCTAAAATACTAAATTCTTGAAAATATAATTCGATAATTTTTATCTTTTGATCAAGTTTTAATTTTAAATAACCTTTTATATCTCTATTTCATTTGTTTCTTTTTACTTTTTTAAATTTAAATTGCATAAAAATGAACCCCTTTCCAAAAAGTCCGGAATTTGGGGTTCACTCTATAATTCAGATGATACTGAAGTTGTTTTAATTAGTGAAAAAGAAGAACATGAATCTACTAAATAAGTAGATTTTTATTTTTTTGTGTTATACTAAATATGAAATTGACAGAGGTATTTGCTTATATAACAAGCAGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTGAATTATAAATAGTTTTTTCTCTAATCCTTTTACTCTGTCGTGAAAGGATTTTTTTATGAAAAAAATATATAAATTTATTGTAGCAAGTTCAAGTATTTTTGCTTTAGGAAGTATTAGTGCTGGTTGCCAATATCAACCTAATATTGAAGAAATAAAAATTAAAATGGAAGGTATTAAATATGCAGATAAAGAAAAGTTAGATGCATATACAAAAGTATTTAATCAAGTTTTTAATATGAAAATGAAAGAAGCAAATTTAAATCAAAAAGTTGTTTCGTTTCATGATTCAGGAAATGGTGAAAGCGCAACACTTAAAGAAGTTAATTTAAACAAATTTCAAGTGGCTTTTCCCGGAGCTTGAAGATATTATCAAGAATATTTGAATAATAAAAATAAAAATGTTGTAGCTTTTATGCAAACAAAAACTAGAGCTTTTAAAACAAAAAGTAGTTTAAATTACAATGATCAAAGTCTTTATGATCCAAGTAAAAGTAATAACTTCGCAACTTTACAAGCTCAATATGAAAATGAATTATTCAATAGTGAAGGTGGCTATGTAAATTGAAGTGACAAAAGCATGGGCGGCTTTGTTGATTATAAATTTTTAAATATGTATGAAGCTGATAAATATGTAGATCATCAAAGAGGATGTATTTGAATTTATGGTACTAATGAAGAAAGAAATTTAATCAAAAAAGCTTGAAATGATAAAGATTGAGAAACTTTTTATAAAAAAGGAATTTATTATGGAAGTAATGATAGTGCATCAAAATATCATTTACCTCAAGCATTATTAAAAAAACAATTTAATTTAGACAAAAACTTTACACTAGATTCTTTTGCAAGTTCAAATTCCAAATATTTTAAAAAAGGAAAAGCTAAAGAATTGGGCAAGGATAATTCTTCAAATTATCATATAGCTTTTGATAATGAGAATTCTTTTGCTTATAGTAATCATGGAAATAAAGCATTAGAAAATAGAGATTTTTATGTTAAAGATAAAAATAAAACTTATGAAATGTTAACTTTAACTGATCCTATTCCTTATAATGTGGCTATAGCAAATAATTCACTAAGTAAAAATGTATTAGAATTATTAGCTCAAACTTATATTGAATTAGCAAATAAAAATAATAATTATGTTGGAAATTTATGAGGTCTAAATCATTATTCAGTTATTAGTAATGAACAAACAGAATTTATAAATAGAATAGAAGCAGTTATGAAACAATAATATGAAAACATACAAAATTGAATTTAAAAATGTAACTTTAGCTTATCAAAATGAAGTGGTCTTAAGCAATTTAAATATCATTTTTGAAAGTGGCAAAACTTATGGAATTTATGGGCTTAGTGGGGTTGGGAAAAGTACTTTGATTAAAGCTATTTTAGAACCAAAATTAATAGCTAAAGGGCAAATATTAATTAATGATCAAGATATCAATAAAATTGATCAAAAGACCTATAAAACAGTTAAAAAAGATATTTCTTTTTTATTTCAAGAGCCTTCTTTAATTGAGACAGATGATGTCATTGAAAATATTAAAAGAAAGTCAAATAATATCTATAATAATTGATTTTACAAAGTTTTTAGAATATTAACTAAAGAACAAAAAAATAAGCTAGCAAAAATCTTAGAATATTTAGATATTTTTGATTATATTAATACACCAGTTAAAGATCTTAGCGGTGGTCAAAAACAAAGAGTGGAACTAGCCTCAATCTTTTTTGAAAATAAAAATATTATTTTGGCTGATGAACCAACAAGTAATTTAGATTTTATTAATACTAAAAAAATATTAAAATATTTGCAAAAATATGTTCATGATAATAATGCATTATTATTATGTAACATTCATGATGTTACTAATTCGCTAGAATATATGGATTATATTTTATTATTAAAAAATAAGAGACTTGTTTTTATTAAACAAGCTAATAATATAAGCGAAAAAGAATTTCAAGAACAATATTAAAGTCTAACTAGTTTAGGCTTTTTATTTTGAATTTCAATCAAATAGTTATGCTTTTGTGATATTTAATTAAGGTTGTAATTAATTCGATGGAAATTAAACAAAGTCAAAAGATTAATATTAAAGCTCCAACTTTTTGTCATTCATTTTTGTTTTGGTAATAATTTAATCTAGCGCCTAAACCAACAGCTGCAAAAGTTCCATAATTAATAATGTTTCTAAAGATTAATTCGAATCTAAAAGCTGCATAACTATAAAAATCTTTAATTACTCTAGGTAAAATAAATTCAAAATATAGAGTTATTTTATTTTTATTTAATAATAAATAATTGCCAATTAATTTTTGATCAATATTATTAATTGATTCAGTATAAAATTTACTCATTGAACGCATTGTAGTAAAAGATAAAGCTAAAATTAAAATGACTTCTCTTGAATAATAAAGATTAATTGATAAATAGAAAAATAAGGTTATTGGTATAGTTCTAAAGAATGCTAAAAGCATTTTACTTATTATTTTCACAAATTTATTATTTGTTTTATCGTTTGCAAAAAAAGAAGCAAAAATTGAAAAGATAAAAGCTAAGAAAATGCTGGCAATAGTAATTTTGATAGTAATTAGTAATTCTTTGAAAATAGAATAACTTTCGCTTTTCAAAATTGAATAATTTGCTTTATAAGTTAATTCACTTCATCTACTTTTAAAAATATCTAAATTAAATTGATATTTAATATTAATAAGTGTAATAACACTCGTTAAAAAAATTAATGAAAAACTAATTATTAAATATAGTTTAACTCGAACTAAACCTTTTATTTTATTGTTAATTTCTAATTTAAGTGGATTAAAGATTAAGTGATTAATTATTAAACTATAAATTTCTAATATTAATAGTATTAGTGCTAAATAAAGAAGTGGAATTGCAATAACGCTATAGTTACCACTTTTAAGAGGATTATTGATTAATTCTCCAATTCCAAAAAGTCCTAAAGTACTTAAAACACTGCTTCATCTAATATTTGATTCAAATGAATAAAGAAATAACACTATTACTTTATTCTTATTACGATAAGAAACATTATTAATAAAAGAAGCAAATTTATTTTGTCCTAGTAAAATTGAATTTCAATAAAAATTAACTTTACTGTTTTCTCATAATTCACATAAATATTTATGCAATCAAAGTCAAGTAAATCAAGTCAATATCAAAGAAGCTGAAAGCAATCTATCAAAGCTTGAAGTAAAAAGAAATACAAAAAAGAATATTGGTAAAGTTCTTAATAGCGTAATTATTATTTTACTTATTATTGGTAATATTTTATTTTTATGTAAATTTCAAGAAGCTAAATAGCTTGTTACTAAAGCTAAAAGAAAACCAAGTAGACTTCCTAATGCAGTATATTGAATGGTACTTCATAAAAAACTTAAAGATAAAATTCATAAATTATTTGCGGGATATGAAGAGTTTATATTATTAAAAGTAAATAATCTTTGTAGATCATTTTTTAATTGTTTTACTGAATTATTGTCCATAATTTTTCAATCAAAAGATAATAATGAATATAAACAAATTATTAGAATAAGCAAAATAAAAGTTACTTTAACTCATCTTTTGAGTTTTTTATAACTTTCATTTTTGCTATTAACATAAATATCAAATAATCATGAATAATGATATTTTTTGTTTTCTATTTGATTTGTTTGCAAAATAAAATCCTTTCATGATAGAGTAAAAGGATTAAAACACTTGAATTCAAAATTAATTGAACTTGCTCCGCAGGTATTAACCTGATCAGCTCTAAGAGTTTTTCTCAATCCACCTGTTTTATAGGTAAATACCTCTGTCAATATATTTATATTTTACCATTAAATTAATAAATAAGCTGTGCTTTTAGAAAATAATTTTTACCTTTTTTGGTGCTTTTTATGGTTATTTTTAAGGCATAATTTTCCTTATGTCCCAAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGGATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAATATAAAATACCTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCGCTTTGGTAACAAAAGTTATATGAGAGCTGTAAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTGGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAAAAAAGACGGCTTAATTAAAAAATTCATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAAGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTAACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCCTATAATTCAAATGCTGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCTAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGAGATAATTATATTGAAGGCAGAAACAAATTAATTAATGAATATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATGGGTTTAAAAAAGCTTTTGTTAATATTAAATCAAGATTAAATGAATTTAATTTAACATTAGAAGCAAACAAAACAACATTAAATGAATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTGATAATATTTCAACATTCTTTGAAATAATTTCAAATCTTAAAGAATATGAAACAAATTATTTAAAAATATTTGAAATTATAAAAAATGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAATATTGCTAATTTCTTATCAAAAGTTTTTGATAATTTAAAAACATTAGACCAATTCGAAGATGTTAAAAAAATAATTGAAGATATTAATAATAACAAACCTAATTTATCAACTAAAGAAATATCTTCAATTGAAAATAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTATTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCAAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATCTGTCTCACAAAATAATGCAAATTGAGATGAAAAATCAAAAATTTGAGCTAAACCTAAAGTTTTAGGCAAAAAACATCTATATGATCGTATTTCAGATTTAGATACTGGAGGATACTCAAAAGGTAATACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCTGAATATTCATGACACCGATATGGAGTAGATAAAGGAAATACTACTAGTGAAATCAAACAAGGTGAAGAAATTGAAAATGATAATTATAAACCTTGACATACAATTATTAACCGTAGTCTTACTGGTAACCAACCAGCAGTTAAATATATAAGTCAAAAATATCCTAATATGAAATCAAATGGTTTTTTACAAGCAGTAGGTGATGATATTTGAAGTGGGCATGATGATCTATATACTTTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAACGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATATAACAATCCAACAAGCAATGGATATTATGAAGGAATATGAGAAAGATGAAGTGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTTGAGTTCATAACAACTATGGAGAATCATATACTCTTCCGGGTCCAGATGGAAATCCAACAGAGAGTGATTTTAATGGTCCTTACTATAGAGCAGCTGCTCATACATCAGATGAAAAATATGATAGTACTAACCCACTATTTGAATATATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGACCATGCTTCAAATTCTTTCTTATTAAAACATAATGGTGAACCTATTTCATGAGTAAATTACATCTTTAAATTTACTCTTGATAACCCACTAGGAAACTTTGATGCTTCATATTACTTATGAAATAGAATGTACTGAAAATCAGAATATGCAATCACCGAAAGATACATTTTGAAAAATAATTCGTCTTCTGAAACAGTTTCAATTTGAGATAAATGATCAAATATCTTTACTACAACAAGTAATAATACATATAGCAAACTTATTTGATAAATTAATGAAATTTAATATTAAATGAACCCCAAATTTCTTTTTCAAAAGGGGTTCATTTAATATTTGAATTAAAAAAAGAAAGATTGTTTAGAATTTTGTGTAAAGTCTCTCCCTTTCTCAATAGAAAGGAATTAACTTTAGATCAACACCTAACAATATTGTAGAACAAATAAGATAATGTCTAAAAAAAACATTATCTTATTTTTAAAATTTGAATTTTAAAATAGGAAAAAAGATCTTGCTATGCAAGAAATCTTAATTCCTCTCCCCTCTCCCTTAATTCATTTAAGGGAGAGGCCTTCTTTGAAGCCAAAGAAGCAAAGCTTTTAAAATTTGAAAAAATTATTCTTCATTTTCAAAATCAATTATTTTAAATTCATTTAAAATGCTAGATCAATTTCTAATTCTTGAATTACTTCATTTTTTTATGATTTGTTGAGATGCTAGAAAAATAATTTTTAAAAGACTATCATCAGATGGGAAAACCCTTTTTGTTTTTGTATATTTTCTAAATACACTATTTAAATTTTCGATTAGATTAGTAGTATAAATAAGCGTTCTTATTTCAGGAGAATAATCAAACATTGTAGAAATTTCAGATCAATTTCGTCTTCATATTGAAATAGATGTAGGATATTTTTTGCCTCATTTATCTTCAAAAATATCCAAATTATCCATTGCATTTTTTACATCATTAGATTGATATACAGATTTTAAATCTTTGCAAAATTCTTTCATATCTTTGTAGTTAACATATTTAACAGAATTTCTAATCATATGTACAATGCAACGTTGTTGCTTACTATCAGGAAAAACAGCTTCTATAGATTGTTGTATACCTTTTAAATTATCTGAACAAATAATATTAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGCAGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGTCCATCACCATTAATACCCATAATAACATATGTTGCTTTCTTAGTTACAACATTATTATCTACAACATGAAAATGTACTGCATCAATATAAATTATTGGATAATATGTTTCAAGTTTTCTTTCTTGTCATAATCTTACATCTTCGACAATTTCATTAGTTAATTGGCTTATTTGTGCTGGAGAATAATTAACGCCATATTTGCTAAACATAAAATCACTTACATCTCTTGTTGGCATTCCTCTAGAATATAAAACAAATATCATATCAACTAATTCAAAAATATCACGTTCATACTTTTTGATGATTATTGGTTCAAATTTTGCTTCACGATCTCTTGGAATGTGACCATATTGACTATTAACATTTTTCTTAGAAAAACCATTTCTATAATTTTCTAAAATTTTTCTGTTATTTCATCAATAACAGGATTTATTGGTTCTCTTTTCATAAATAAAAAATCCTTTCTTGATAATATTTTATCTATTAAGAAAGGATTTTAAAACTTTACACAAAACTTTTTGTAGTCCAACTTAAAAATAATAATTACTTTTTGTATGGATTAGTCATAATATTTTTTTAATCCAAATATTAAATGAACCCCTTTTTGAAAAAGGAATTTAGGGTTCATTATAATATTAAATTTCATTAATTTATCAAATAAGTTTGCTATATGTATTATTACTTGTTGTAGTAAAGATATTTGATCATTTATCTCAAATTGAAGCTGTTTCAGAAGATTTATTTTTCAAAATGTATCTTTCGGTGATTGCATATTCTGATTTTCAGTACATTCTATTTCATATGTAGTATGAAGCATTAAAGTTTCCTAGCGGGTTATCAAGAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGAGCTCCATTATGTTTTAATAAGAAATGAACTGAATTATGGTCTACTGTTCTTAAATATGAAAATAATGGATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTTTCATATTTTTCATCTGATTTTCCATTTTCTGTTCTATAGTAAGGACCATTGTAATCACTTTTTACTAGATTTCCATCTGGACCCGGAAGAGGACCGTATCAATTCCCTTTTTCTTCATCATAAACTATAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTGTTGTATTTAATAAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCCTTGAAAATCATTTTTAAACATTATTTTAGAATCTGATATTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTTTTCTCATATTCTATTAATTTGGCCAAAGTATATAGATCATCGTGTCCACTTCAAATATCATCATTTACTGCTTTTATACAATCTGCTCAATCTTTATCAGGATATGTTTGTTTTATATATTTAACTGTTGGTTTACCATGATCACCAGGGTCAAAAATTGTATGTCAAGGCTTATAAGGTTCATTTTCAATTTGTTTACCTTGTTCAATTTCACTAGTACTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAAACTTTAGGTTTAGCTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTAAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTTAACAGCTGATAAATAATCATTTTCTAAATAATTTTTAATTAAATCATTATTTGTAAAATCAACACCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACACCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAACAGCACTAAAGTTTTCTGTAGAATTAACATTTTTATAAGTTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCCACATTTGCCATTGTTGCTAATTTCGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATCGATGAAATAAAATTATTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAATTTGTTTCATATTTTTTAAGATTTGAAATTATTTCAAAGAATGCTGAAATATTATTAATTTTGTTGTCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTAGTTTGTATATCAATTATTGATTTATAAGAAGTTTCAAATAAATTAAATCCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAATGCTTTTTTAAATCCACTTTTTATTGAATCTAATTCATTTTTGCTATTAACATATTCATTAATTAATTTGTTTCTGCTTTCAATATACTCATCTCATTTGAGAGAATTTAATTCAATTTTAGATTTTTGTACAAATTCATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTCGATTGAATCAACATTTGAATTATAAGCATTTAAAAATTCTTTAATAACAGATTGAATAGAAGTTGTCAAATTATTTTCGTCATTTAAAATAGCTTGAGTTTTGTTAATAAATTCGTCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAACAAATGAATTAAATTCATTAAGACCATTAATAATAGATGTTAATGATTTGCTGCTTAAGTCTAGAATTTGAGATTTGTTAAATAATTTTGTATTTTCAATTGTTTTGACAAAAGTATTAACATAACGATTTATATATTCTTTTGTATTTCCATATGATTGTTCCAATGTTTTACATGCCTTAATAATATCTTCTATAATAACACTTCTATTTACTGAAACACCGTTTTTTTCTAATATAACGTCATAATTATTAGAGTCAAATCTTGACAATAAAATCTTTTTAACAGCATTGATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTAGATATGTTTGACATGAATTTTTTAATTAAACCATCTTTATTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCATTATCAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTGTTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAATGTTTTGATAATCTTTAATTTTGAGTTCTAAAGAATCTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGTGGTTTTTAAAGTTAATAAATCATTACTACGAACAATAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTATCATCTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTGTGTTTAGCAGCTCTTATATAACTTTTGTTACCAAAGTGGCGGCCAACATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAATCAATTTGAGTTTGGCACAAACTTAGTTCATTTCAAAGACTAGAAAGTCATTCTTGATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAAATATTTTATGCTGTTTTCAATTCACAATTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCGCCAACAACCCCAGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAACATAAAAAAATTAAATTTTCGGGACATAAGGAAAATTATGTCTTAAAAATAACCATAAAAAGTACCAAAAAAAGGCAAAAATTATTTTCTAAAAATACTATAACTTATAAAAGTAAGACATACCATTAAAGATTTTTAAAATTTTGAAAAAAATTTTATTCTTCATTTTCAAAATCAATAATTTTAAATTCATTTAATATGCTAGATCAATTTCTAATTCTTGAATTACTTCATTTTTTTATGATTTGTTGAGATGCTAGAAAAAGAATTTTTAAAAGACTATCATCAGATGGGAAAACCTTTTTTGTTTTTGCATATTTTCTAAATACACTATTTAAATTTTCGATTGGATTAGTGGTATAAATGAGCGTTCTTATTTCAAGGGAGTAATCAAACATTGTAGAAATTTCAGATCAGTTTTGTCTTCATATTGAAATAGATGTAGGATATTTTTTGCCTAATTTATCTTCAAAAATATCCAAATTATCCATTGCATTTTTTGCATCATTAGATTGATATACAGATTTTAAATCTTTGCAAAATTCTTTCATATCATTTAACAGAATTTCTAATCATATGTACAATGCAACGTTGTTGCTTACTATTAGGAAAAACAACTTCTATAGATTGTTGTATACCTTTTAAATTATCTGAGCAAATAATATCAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGTCGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGCCCATCACTATTAATACCCATAATAACATATGTAGCTTTCTTAGTTACAACATTATTATCTACAACATGAAAATGTACTGCATCAATATAAATTATTGAATAATATGTTTCAAGTTTTCTTTCTTGTCATAATCTTGCGTCTTCGACAATTTCGTTAGTTAATTGACTTATTTGTGCTGGAGAATAATTAACACCATATTTGCTAAACATAAAATCATTTACATCTCTTGTTGACATTCCTCTAGAATATAAAGCAAATATCATATCAACTAATTCAGAAATATCACGTTCACACTTCTTGATGATTATAGGTTCAATTTTTGCTTCACGATCTCTTGGAATGTCAATTTCCATTTGGCCATATTGACTATTAACATTTTTCTTAAAAAAATCATTTCTATAATTTTTTTTGAACTTTATTATCATTTTTTCGTAACCTATAAAATTATCAAATTCTTTGTTCATCATTTTTTGGATTGTATTTTGAAATACTTCTTTCATAGCCATATTAAGATCACCAGTAGTAACTTGGTTTTAATTTTCTAAATTTTTTTGTTATTTCATCAATAGCAGGATTTATTAACCTTCTTTTCATAAATAAAAAAATCCTTTCTTAATTGATAAAATATTATCAGGAAATGATTTTAAGACTTTACACAAAATTTTTTATAGTTCCCAATTTTAAAAAACAGGCAAGACCTGTTGGTGATGTAAATTATTAGTGGGATAAGTTAGAAATTTAAAAGAGAAAACGGCATTTGGTATGAACCCCAAAAATTGGACTTTTTAATTATGTTTGGACTTATTCCTGTATTGGACAGGACTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTCCTAAAGCAGTTTTAAATTCTTTAAAACTATTAAATTTCTTTTCTTCTCCAAATCAAAATTCTCTTTTTATAACACTAAATAAACATTCAGTAGGACTATTGTCTAAACAATTTCCCTTTCTTGACATGCTTTGAGTTGTTTGTTTTTCTTTCAAATAATCAATATAATCTTGATGAGTGTATTGTCATCCTTGATCAGAATGTAATAATACATTTTTTAAGCTGTGGCCATTCTCTTCTACGTTTTCTAACATTTTACCAACCATTCTTAAATTAGGCGATTTAGAAATCGAATAACCTAAAATCTCTCTGTTTTCAAAATCAACTACAGGAGAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTGTTCCTAGTATTTTCAAATATTTATTATTTGAAAAATTTCTTTTGTATCTGAAGAAATCTTCTGTGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTGTGAGCATCACCTTTGAATGAAGAATATTTATTTTTTCTTCTTAGCGTTTTGCCGAAAATATTCATTGCTTTCATTAATTTTTGAACTTTTTTATGATTGATTATTATTCCTTTATTTCTTAAAGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAAAATAACCTTTTTTAAATTTTCATCTTTATCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAGTTGACTTTTTCAATTTAATTTTTTCTAATATTAATTTAATAAAAAATAGCTTTTTAAATTCTTGCCTTAGTTCAATTGTTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAGGCGCTCACTTTTTTTGCTATCGCATTCTCCAATTTGAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCGGAACTAATATAAAAAGGTGTGTTATAAGATTCTCTACGTTTTCTTATTTCGTAATTATTTTATCATAATCCATTTTGTAGCAAAGGAAAATACTGCATGTGAAGAGCATGCAGTTTCCTTTTGCGCGCACACGCGCTACTTGAATAGTACAAGATAAAGACACTACAAAGCTATTTACCATAGCGTGTTTTATCTTGAAATGATTTATTCATAGAATAAATCAAAAATTAATATTTTCATATTTTCTAGGCGCATGCACTTTTTTCGTTTTTGCCTAAATTCGCATGCACTTTTACATGCACTTTAACTTGTATATTTTTATTTTAAAAATATATTAATACTGCTTTTGCGCATGCACTTTGTTAAAAATACTGCTTAAATGAAAAATAAGTTTGCTTTTGTTTCCATATTTTTTGCAATGCTTAACTGTATTGTTTTTAGTGAAAATGTAGCAAATTTCTTTGAGCAAAACCTCTTTAAATCGTGCCCAACTACCGTTTCAGCACAGCCTCCAAAGTAGTTTTTAACTAGCACTGAGTTTCAAATATATTCTCTATTTTCTTTATAATATTTCTTGAACTTTTTAGCATTAGCATATACAGTTTTATTTTTCTCTTGCTTTGTTATTCACTCTAAAACACTGTCAAGTTCTTTCTCAAATTCATAAATTAATCCTTTAGAAAACAAGTTGTCTAACACGCGATAAACATCTTTATAATCCCTAAAAAATCTCTTATTACTTACATTAATTTTTTTGCTATATCCAAAAGCTTTAAACATATTTGCTTTGAAATGAAATGGATCATAAACTCTCGTTGATTTTAAATTATCAGCTATTGTAGTTATGAATTTTGCACCATCTCCAGAGACAATAATTTCACAATTTTCTTTATATAAACTGTTTAAACTTTCTCTAATTTTGTTTTCAAAATCTTCTTCACTATTGAAGTTTTTAAACTCACTTGCAAGTGGTAAAAACTGCATAATAGAGGTCTTATTTTCTATGGCTTTTACACTACTTCCGCTAAGCAAAGTATGTATAGTCGCCATACGCACTCTAACCTTTATTTTTCTACCTTTAAAAGACACTCTAATATAAGCATCATCAATATCTATTTGTATAGTCTTGTACTCTAGATTTTTATAGTCTTTTAAAGTCAAATTATTTAATACTTCTTTCTTTAATTGTTCCCTATGGTAATAATAAACGTGATAAGGAATAAGTAGCTTACTATTATCACCTTTTGAAGCGTATATATTGTTTTCAACACATTTTTCGACGCACTCTTTATAATATTTATTTCTACCGTTATCAGCTAATCCTTCACTCGGATAAATAACTAGTCTTTTGCCTGTAGTTTTATCTTTATACATGTAGTAAATAATTCTTACTGTCTCGCCCCAAATAAAAATATCTCTTTCTATTTTTCTTTCAACATTATATTTAATTTTACGCTCTTCGCTATTCTTAAAAACTTCTGCTTGAGCATTTCAATTGTCAGCTAAAGTTTTACTCATTTTGTTCTTCACAGCGCCCTCCATAATCAATTAAAAACGACGTTTTCACGCCGTTAATAATTTTATATGTTTTACTTCAGCTTCGTTTTGTCTTTGTCTGTTTTGCTTTCTTTTATTGGTATTTCCTTAGCTACTTTATCCTTAGCTTTTTCGATATTTTTATTATCGTATGGATCAATATAGCTTGATTCACTTTTGCTACTTTTAGATAAAAAACCTATATTTTCAAAAGAATATATATTAACGTTATCCTTCGTCTTATCTAAGTCTTTTCAATCATTATGAGCAATTTCTCTAATAAAATAATTACTAGTTTCTTTATCTAATTCATACTCAAAATCATTTTGATTAATTATATTTTTCTCTTTACCAATACATAATTTAATATTCTTTAAAGCTTCTTTTGTATATTTTGTATTAGAAGTGTTTGTGTTAATTAGGAATTGGGATTTTGAAAAATTATTAGCAAAATAGTTTTTTGTTGCACGCTTAACTTTATTACTATTATTAGACAATGTAGATAAAAGCTGATTATTTATTAACTCATCGTTAGTATATAAAACTAAATTGTGCTTTAAATTATTTGATATCTTTATTATATTTAAATCGGGAGGAATTGCATTTATACGGCGACTTAGGTCATCGACTATCAATGATTTATCATTTTTATTTAATACAGAAGTATCTTGATAAATTGCTTTATCTATATTACTTTCAAGCAATTCCTTATCAATAATAAATATATCCTTAGTAACTACTACACTTTCATGATTCTTTCAATATTCGTTGGTTTGATAAGAGCTATTATCAATTATTAAATTATATTTGCTAGAACTTTTTCTTGCCGTTTGAAGTCTTATACAATAATAAGTTTTATTTTTTGTATTTTCAAAAATTATCAAAGGATGAATTTTAAATTTTGTATCGATATTTAGATGAAAAATATCTTCTAATAAAGGATTTGTAATTTTTAATGGTCTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAAGAGGATTAAACCTCTTTTTAAGACGAACTAGAGTCTAAGGCGTATCATCAAGCGCTTAAAGATAAAATATGAGCTGCTTGCTTGTTCGATTACTCATATAACAAAATCCTTCTTAGACTATTGTTTAAATTATACTACAAATAAATAAAAAAATTAATAATTTTTCAAATCTTATTTGCATTGTTTACTCTTAAACAAATTGATAATCTTTTACTAAAAAATTATAAAAGGGTGGGCTTGAATTTTTCCGTTATTTAAAACTTTGCCATCAATAGGTAGTTTGAAAATATTTTCTAATAATGGGTTATTAATTTTTAAAGGTTTTCATAAATCTAAAGACATAATAAAATTATACAAAAAAAGAGGTATAAAACCTCTTTTTAAGACGACCCAGCCTAGAGTAATTGTAGCTACACGCTAAGCTTGCTACTGTTCGATTACTTACGTGTATCTCTCTAGGTTCATATGTCTACATTATATAGTAAAAAAACAATATTTTATAAAATTCTTAACTTCACCCATATTTTCTTAATAATGTTTTAGTTAAAAATATAGTGCCTAACATTTTTTTATTTTGCAAATAATTTATTAATTTCTTCATCGCTAAGAACAACCGGCTTAGCCTTAATTCTGTCTTTTTTATAGATTTGTATTTCTTTGAATTTCTTAATTGAAACTTGTTTCTTGCTCTTGCCAGCTTTAAAGCCTTTTTTATTCAAGTATCATCTAATACCATATATGCCACCAACAGCAAAACCTAAGGCAAGCATAACCGATACAATTCAAATTCAATAATTACTTTTATGGCTTGCTTTTGTTTTATCAACATCTAAATCTTCACCATTTGGCTTTTTATGCTTATCGTTTTGTATTTCTTTAAATCATAATTTATCATCAAAAGCATTATAATTTGCCTCAATATTATTCACCACTATAGCATAGGCAAAATTAATATTATTGCCTTCTTTAACTGGTAATAAAACAAAATACTTGCCTCTAGCTTTATAATCTAAGCTTTTATTAAAGTTATCGTTTTGTATTTCTTTAATTGCCTTTTCTAAAGCTTCGTGGTACTTCTTAAATAGCTCAGTATCATTTATTTGATACGGAATAGGAACTTTATGTCCTTCAATTTCTCAAGTATTATATTCAGCCATTACCTTTGCTTTAGTTTCTTCATAAACTTTTTTGAATTTTTCATATTTAGCTTCATTTAATTTATCAATATCAGCTACTAAACCAAGTAAATAGAATGAAGCATTTACAAGTGAAACTAAATAATCTTTTTTAAACTCGATACCTGAAGCAATCGTTATCTCATTTAACTTATTTATAATATCTTTGGTAATTTTCTCAGGTTCTTTTTGCGATATAACAATAGGATTTTTTACTAGTGTATCATTAAGTAGTGATAAATCAAATGCTGGTGCTTCAGTATCATTAATAATATGAATCTTAGTGCTTGAAATTAAATTAGCATTAATACCTTTTATCTTAATATCAATACCATCTTTAAACTTATTCCCCTGTACTAGTAAATCTAAAACGTTATCTAGTCCTTCTACTTTAATATCTTTATTCAATAACATTTCTATGCCAGTTTTGTTTTTGTATAAGTTGAATTGTTCTTCTATTAGCCCTATCAACTTATTTCTAATCTTTTCTTTTAATTCTTTTGAATCATTACTAATATTCTTTCCCATTTCAATTTCGAAGTGTTTTTGAATGTCTAGTGATGATAAATCAATAGGAGGATAGTACACGTGGTACGCACTATTTTTCAAATTAAATGTTATGCTGTGATTTATGTATTTACCCGTTCCACGCAAAGTAAAAGCTCATCCCTTACCGTCATTATCTGGTACATTAATATCTTTAATATAAACTTTTAAAGCCCTATCCAATCAACGCTTTTCGCTTTCTGGCGTATTTCAATTAGCTATTACCCAGCCACCATTAATTTCATTTTCAGTTATTCCAATTGCCTTTAATTTAGCACTATTAGCAATATTTCTTTTAACAAAATTAAGCACGATTTCTTTAACTTGTCTTCTATAATTTTCTTTAATAGCTTCATTAGTTTCATCTCCATAATTCAAGCCATTAAGATTTTCTAATTTACCTCAAGTTATTTTATCTTTAAAAATATTTTCATACTCGTTTCCTGTTTCTACATTAAACTTTGACGCTTGGTCTACAAAGTTTTTATATAGTGAAATCATCTTGTCATATCCTAATTTACCCAAGTCATCATTTTGGATACCACAAGCTTCATTTAAGTAATTTTGAAATAGTGTAGTTATTCAATTGCCACCATCTAAAAATGCTTCAAAATAATCTTCACTTTGATTATTAATTTTTTCTATCTTGTTTTCAATTGAATCCAAGAAAAAACTTTTTGGCTCGCTCTTTGCATCAATTAATACCAAATTAACATTTGATATTGATTTTTCATTTACAGTAAATAATAGCCAAATGCCTTCCTCAGACATATAACTATATTCACTTGTCCCATTATTTACCGGTATTTCAATTAGTTTTTGTTCTTTCCTACCAATCTCAACGTATTTGTCATTTTCTTTTCTAAACAACAAACACTTATAGTATCTTGAAGATGACTCTAAAACTTTATCAACCACCAAGTTTCTTAATGCCCCCTTACCAAGCACATTAGCTTCAGCATAGATTCCAAGATCTGCTTTATCTGTAGTTATTTTAATATTAGGATATACAAAACGAAGTTGTTCTTTTAATTCATCACTAAACAACTTATTTTTAATTCAAACAATCTGCTTTTTAGTTCCTGTTTCTTTATCAATACTTGGATCATAATTCTTATTTACTATTAATTTGCCTTCTTTATCCTTTAACTCTTTTCCCTTTGAATCAAGCATATATGGAGAAATAATTTGTTTTTGATCCATATTCTTTTCCGGCTCTCAAGCATAATATTTTATGTTTTGTTTAAACTGATTTCCAATTACTTCATAAATAACTACATACTTTTCTTCCGGCTCTCCACTATTATTTGAGCCTTTCTTGTATCTATAAACTTCAACAAGATATTCATTTTTTGCAACATCGCCTTCGCCATTACCGCTTCCCGAGTTTTTATCAAAAGGTATTTTAGATGCATCATTAACATTAACTCTTTCATCAATTAATTTCTTTTCAAATATTCTATTTAAAACACTAACCTTTTGCTTATTTACCATTAAAACTTCAGTTTCATCCTCTGAAGCTTTAAAAGAAATATTAATTGGCCCTCTAACTACATACCTTCCACCAAATCATGGCTTACCGACAAAATCTTTTTCAAGTTCTTTATTGCTTACCCTTTCAGGTTCGTCTCTTTTAATTAGATTTTTTAAATCGCCACCATTAGCTTTTGTCTTTGCTTCTATTTGACTTATACTTCCATCAGGATTAGTAATAGAAGTTGATATAGTTAACCGTGAAGCTAACGAAGCTCCACTAAGTCTTTTATACAAATCTGATTCTAAAATATTTACCTTTAATGAATCAAAAACTTTAAAATTACCTAAAACGATATCTTTTAAAACCCTTAAAAATTGATTCCACTTCCCAACAGCTTCAGCTTTAATGTTTTCTTTTGTTCAGTCTTCAATATTATTAAGAATCTTATCCAAGTCTTTATCGATAATTTCCTTATCATCAATCTTGACTGATAAAACCTTAATATAAACATCAATTTTGTTCTTTTCACCAACAGCTTCGTTTTTTATCTTGTAATCATATTTGTATTTAACTTTAATTCCACTAGCATACTCAAACTCATATTCTTTATCGAATATTTTGTTTAAATGTTCTATATTCGATTTGCCTCCATTGTCTTTAAAAACTCCATCAACTGATGTAGTGAAATCGCCCAAGGTATCGCTTAATAAATCTATAGAGTAGTGATTTTGGTCAATTTTCTTATTTACCCCATATTCAAATCTATTCAAACAATTATCACTTGAAACACACTCTTTTAAAGCCTCTATGAATCAGCCAACTTTAGCATATTCTAGATTATTTAATACTTTATTATTATAGTACCTTACATCATACATTCCTTCAATTTCACAATCAAAATCATGAAGCCCCCACAAAAACATTTTATTTTTATCATCAGTTAGCCCACTATACACCCTTTTATCTTTATCAGTTTCAAGATTATTTAAAAACTCTAATGTTTTTTGAGAAACTTTATATTCAGCAAACAGGTTAGTAAAACTAGCACCTTTATGTTCTTCCATAAAGTTGTTTTTGTCTCATTTTATTAATTGTTCTATAAAATTAGAAGCAAAAATTACTCAATTCTCTTTTTCACGAGGATGCAAATAATTTAGATAATATTTATCATCGTATCAAAACCAGCCATAACTATTATGATTACTAGGATTTGTGTAATAGGCTTTTTCATAACGCACTTCAAAAGAGCCTAAAGTTGGCCTAGAAACTATTGACGTAATTTTATTTTTTACTGGGCCAGTTTTATGATAACCAACCCATTGGTCCTGTGCATACAATAATCCTTTATCACATAGTTGTGACCATTTTACTTGATACCTATTTTGTAGTCTTTCATTTTTAGCAATTTCATCATCTTCCATACTAGTAAAATTGGAAAATAAACTATTAGTATCAATCTTATGTTTTTCTTCAGGAGCTTTAAAATCTTTTCCATAACCTAGAAGTTTTTTATTAGTATTTAACGATAAATTAAGTACTGACGGAATCGCAATACTCAATACACTCAATGGAATTAATTTTAACATCTTTTTCTTATTCATTTTTTCCTCATTCTTCGCCCGTGCCTCCGCGTGCATATTAGTTATGCCCGTGTGCGCACGTGTGCATGTTTAAAGACTATTTTATTTTTTAAAAAACTCACTTAAATCAAAATCACCAAAATCTTCATCCTCTTTAGCATTATTTAATTCTGAAGCTTCAAGTTTTGCATTTTCTTTTTCAAACACTTTTTCAATTTCATTGTCTTCGAATTGTTCTGCAAACTTGCTTAATGAATTGTCTTCATTTCTTACCTTTTTAATTACCTTCTTATACCATGCAGAAGCCGATTCATTCATTCTTTCACGCAAACGCTTTGCATATTCTGGCTCTTTAAGCAAATTATTGTTAGGTTCATCTAACATTCCTTTATCGCTTATAAGAGCATTTAACAACATATTGACTTTTAAATCAAGCAAAACCAAATCAATATTTTGCTTGTTAATTTCATTTATAATCTTGCGATAAAATGGAGTCAAAGACGCTCAAGATACTTTTCTTAATGCATAAAATAAATCATTTTTGAAATCTTTAGCTATTTCCTCTTTCTCTTGTTTAATTAAAGCGTTGCGAATTATATCTTTAAGCTCTACATACGGAGTAGAGCCACTTTGTTTAATTCTTTTTACCCATCTTTCGTATAACGCCACATCATCTGGTTCACTAAGACGCGTAGAAATACATTTAGAGCCGTTTTTTGAGGCTCTAGAGCTTTTTACATATAATTCACCTTTTTTATTTTCTTGACTAATTTCTCATGTTTTCATCTTCCCCCTTTTCAAAGAATAATTCCTTTTCCTTTGGATTGTAATGTAGATTAATTTTGTATCTAAGTGCATTACTCATACTTGCTAAAACTTTCCCCACTCTTCTTCCTGAAATAAACTTAACTTCAGAATCTAGTAATGGAGCTTCAGCCGTTTTGTATAACTTTTTAACCGCCTCAATATCATCTCCTACTAACCCTAGAAAAAATGAATATTGACAGTTCCCCGTAATCTTTTTAGCAATTTGTTCAACTGCCGTGCTTGAAGCATAATCACTAGGATTTTGCGTGGTATGTATTGTCCCGCAATCAAGCTTACGCACAAGCTTTGTAGTATCTGATATATAGTTCAAAGTAGTTATATTCTTTTCACTAATATACAAGTGTTCTTCATCAATACAAAATGAACAATAACGTGTTAACTTTTGTATTTCTTTCTTGCTAATTAAACGAATCTTATTTTTTTCTTTATATTCGTTAATTCTTTTCTTGTTATTAAAAGCATTATCAAAGATAAAGCCATTCACCAAGCTAATTAAAACCATAATGCCAATTCTTGCATTTACGCTACCTTCAGTATCGATTAAATTACTTGTTTTAAAAACAACCAAATCGTTTTCAATATTGATATTGGTGTGACCATTATATAACGCACCCAAAGTTCCATATCTTCCAAATTTACCCTCTAATAATTCAGCCATATCTAAAACCTTTTTAGTATAGCCTTCTTTATCGTGATGGTCTTTAGGAGCTACAAACTTTTTCATTTGCTTAATCAAATCATCCATAGTTGGTCATCCATCGTTTTTGAGTTCTTTAACTTCCTTTAATCTATAAATGCCCCAACTTTGATATAACTTAATAATTTCAAGTTGCAAAATGTTCTTCTTCATTTCATTCATATCATCAAAAATGAAACTAAAGAACTTTTCTAACCAAAGCAAATGATTATTAATTAAAGTATTGAAATTAACATTGGCATCATCTTCTTCACGCGCAATAAATGATTTGACTTGTAATGGATTAATAACCGTTCCATCTCCAGAACCTAAATCAACTATTTGGCCTCCCATTTTATTTGCCCATTTAACATATTCAGCTTGCGGATCAATAATAATTGCTATATTCCCCGCAGCCAGTGATGAAGTAAGAATCTTCTTGGTTAAAGTACTTTTGCCCATTCCTGAAGTTCCCAATATCATCACATTATGATTTGCTCTACTTGAAGTCAAGCTAAACATATTTCATACAATAGGACTATCATTTCCTCCCACTTCATTTCCAAGCAACAAGTTGTTTCCGTCATTAAGTGGCAGATTTTTCAATGGATAGCCTATTGCGTTATTTATACTTGTGATTTGATAGTACTCTCTATCTAATTTGCTTAATGGATAATTAGACATATCTAAAAATCCTTCAAATTGTCTAAAGGTCAAATCATTAATTCCAAAATTAGATCTTGTAAAACCATTCTTAATTTTCAAATTATTTTGCTTCAATTCTTCAGCATTTTCCGCTTCAGCTAAAACAAAAATATTTGAATCAAATAACTTAAAATTATCCGCTTGTATTTGGTCAATCATAACCGCAATCGATTCTTCCTCTAATGCTGAACCGAATCTATTAATAATTGAATTATCCAAATTACCAGCGTCCATATTTTTCTTATTCGCTTTATCGATTATCTTATTTACAACATTTGGATCGTTATATGGAAAATTGTTTCACACAACTGTTCCCGATACATCGAATAACTTCTTTAATCACTCGTTATTCAAGCGTATTGGAATGTTCCCAGCACATTTAACGCTATACAACTTATCATCAATTTTTAATGAATTGCTTTTAAACTCCACCTTTTCATACTTGAACAAGCGGTCCAAACTATATTCACTTTCGTAAATATCACTTTCATTTAAAAACGTTTCAATATCTTCTTCGCTTTTATTAAAGTGATTAAGTTTATTCAAAAATGATAATAACTCAAAGCCACTTAGCTTTTCATAGCTAATAACTTCATAATCTTCAGCCTTTTGAATAAATGTCGAATAACACTCATCCAATTCTTCTAATGTTGGAGCATTTAAAATTAAATAAAATGTTTCAATCAAATTGCTTTTAACTTCATTCTTAAAATCAAGTTCATTAGCTTTAAAATAACTATTTCAAAACTCTTTTGTTTCTCCACCGCTATTTAACATCTTTTTACTTTGAATCGTGTTTATATAATTTAAATTACGTCTGTAATCAATTTGTTCATCTAATTTCACTATGCTAAATTTATATTTAAAACTATCCAATATTCTTGCAAAGTTTTCAATCAAAACCACTTTAGTATTTTCATTTTCAGCTCATATATTTTTTCCACCTAATTTAAAAACACTAAAGTAATTACTATGTTCACTCTTTTGAAACAACTTATTTGAAAATGACTTGTTTTTAACAACATCGTTTTCTACTAATGTTGAATATGGATTTAAATCAGAACTATCTCTTACATTCTTGTCATCCACCACATTAGAATACTTCTTTGGCATCGCCTTAAATTTAAACATTCTAATTAATAATTCATATATCTTGCAATTATTCTTATGTGTTGGTAATATCAAAATAAACAATAAAACCAAAAGTACACTAAAAACAATTCATTTATAAGTCTGCTTAACTGGATACAAAGCAACAAATGAAATAAATCAAGATATACAAGTTATTACAAAAAACTCGATAATATCTTTTCAACTAAATCTTCTTGTGGCTTTTGCCTTCTTATATTTTAATCGTTTAGGTTGTAGCACATTTCTCCCTTCTCATTATTCTTTAGGTTTAAGGAATAATCCTTTTCAGCCCATCTTCTTTACATTTTCAGCAAGCTGTGCTCCACCTTGAGCAGCTCTACCCGTTTTTGCAGCACTATTTCCCGCAGCTGAAGCCGATTTAATAGTGTTTTTACTACTTACACTAGTTCCAATAAATGAAGTTATAATTTCGCTTATTTCGCTGTATTTTAACGCTGTAGCACCATAAATAATTATTGAAGCTAAAGCTTGTAATACTCATCCAAAACCATTTGTTGATGATGCAATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAAAACAACCTATGCAAGCCATTTATAAAAGCAACAACAATATCATTCCCTTCGCCAATCATCAATGTAATTATTTTTTGTACAAATAGATTAAAAAGTAAAATAGTAATTTGAAATGCTAAAAGTATAAACAAACTTTTAACACTAAATACCAACGCACTTCTCATTAGTGATACACTCTTTTCGCTTTCACTAAAACTGAACTTTATAATCAGCACAAAAAACATCACCAGTCATATAACCGCCGAAACTATCGCAAATCTAACATACATATATGGGCTTTGATGGTCCAAAATGTTTTGTCTCGGCATTATGCCAAAAATAACAAAGTTTAATAAATCGAAAGTTGCTAGTTTAACAACCGATAATAGAATCGCTAATAATATAAAAACAATTAGCGATACAAAAAATAGCAATAAATACAATACATATGTTGGACCATATTTGAAAATCTCCATCACACGGGTAAAAAATCCACTCATTAGCTAGTCTTTCCCCCGCTTGCTTGAATACTAATAACTAGTTCAAATATTCCTCAACCCACTATAACTAGAATTAATACAATTCCAGCTCACAAAATCTTTTTATTGTTTTGCTTTCTTTTTTGTGGATCATCTTCATGTGAGTTTTGTATCCCCGCCACAATCGAGGTAATAATAACGCTAATAACAGCCACCACAGCCACAAGTCCAAATATGATATGTCCAACTTTCTGTACTTGCTCTGTTAAATTACTTAATCCTTCTGTATCAATAGGTTTCATTTTTAATTTCTCCTTTTTGTCTTAATAAATTAATACAAAAAAACTAATAGAAAAACATCTATTAGAAAAATAGTTCTTTGCACACATTATTATGTAGTAAAGTTAGCATAATTTTTAATCTAAAGTGCTTGTATCCAAGCTTCCATTAACTCTATTATTCCAAATTGCAATTCAGCTATGAAACTTACTTTTAGCTTCTTTTTTATATTCAGCCTCTTGCATTTTATTCATAAATTTAGTGTTTAATTCAAGTTCTTTAAAACTTTTATCTTTAGCTTTAAAAACAGTATTTGTAAAAAAGTTTATGTATTCGTAAATTGGAGCATATGGTGTATTGCCTGACTTTTGATTATCATATTCAGTAAAACTATATGGCACTTCTCAATCATTAGCCCCATACATAGGCATTAACACATCATATAGTGTTCTATACGCCCATACGTAGCTATATTCATAATCAGCAAATTGATTATCCCCAATAGCTACTGGAAACATCTTAACTAAGGTTTTTTTCTCTTTGCCTAATTCTTCCATTTCCACGCCAAAGACTGCTCCGTATTTAAGAACATCAAGCAATGCATTTGTTGCTTTGTCCAAGTACTCTTTAAAATAATTTCTATATACTTGTTCAATTTCAAACCACTTCTTATTTCCAGCTTGAATAGTTCCAAACATTTGCAATAACTCTAAATACTTTTTATAAGCTTCGTTATTAACTAACGTTAGCTTAACCATCTTACCAGCCATCCAAGCAAAACTACTTAAATCATTTCAAAGATTATAATCATCACCCGTAGGCAATTGCATTATGAAATCTTTGTTTTGTTTTGCAGTCTCATAAATCTTAAAGCCTTCATGGTATTTTTCAACATTACTTCTTGTAAAAGCTAAATAAAACCATTTTGAATAATCTTTTCCTAAGTCGAAATAATTATTCCACATTCCTTGTTTATATAAACCATCAATAGCAATATCTTTAAATGTCTTGCTTGTGCCTTTAAGAGCAAAATCATTGATGAATTGTTCATAGTAATTATCAAGCTTCATATCAAAATAATAGTGATAGTGTTTTCTTACTTCCTTTTGGTCCTCAAACACTCATTTATTACCATGATAACTATCCTTATAGGCAAATACATCTTTTTGCGATCCTTCTAAATAACTAGCCATTGAATTAATATAATTCTTTGAATCCACAAGACCTAAATCTTTATATTCAGGATTCAAAAAGTTTCTCATCATATTATTCAACTTCACCGGCGCGCTATAATCAACATCGAATAAATCTTTAATTTCGTTTAATTCAGGTTGGCTAGTATACCAGTCTTTATCCAAGTCTAAACGAACATTGATTTTAGGCATATACTCGTTCTTTTTTCAATTATCATTTCTATACACATCGGCATCATATTTTTGCTCATTATGCAATACTTGCGCAAAATAATATTGATTGTCCTTCTTTAGAAAATCTTTTGGATTCTTTTCTGCATCATAATGTACATAGCCATCCACATCGCTCCAATCCGTGCTTTCTCCGCCACTAGGGTTTGGATTAGGGTTCGGATTTGGATTTGGAGGTTGATGTGGCTCAAAAATAGGACCAAAACAACTCGTCGTTAAACTTGCTAAAGGAACTATCGTTCCAATTGCCATAAACTTCATAAACTTTTTACTTTTTTTCATAATAATACCTTTACATAATAATTTTAATGCAAATCACTATTCTTCTTACTTTGTTTTCAGTATTCTATTCGTTTTTTCACTCAATATACTGGATATATGAAAGTCGCCATTCCAAGAAATAAAATTATTCCTTCGATAACTCCATACTTAGTTTCTGTCAATTCTTTATCAACATACATTCCATTAACGGCCACAAAAGATAAACTTAAAATCATCAGCATTAATATCCAGAATACTATGTAAATAATAAAAAACTTAAATTTCAAATAGTTTTTCATTACAATGTTTGACCGTGTTTTTTAGTTTCATCTTTTACTTCAACATCTTTGTTAAAAGCTTCTTCTTCGTATTTATCATAGACGCTTTGACACAATGGATCCATACCAATATATTGTTTAAAAGGTTCCATCATTTCATCCTTTTTCTTTAGTCTCGCTTTAACAACCTTTTCTTTTTCCTCCTTAGGTAATTTTGTTTCATCTTTATAAATAATATCTTCATCATTACCTTCAGCGTTTCTATCAACTTTTTTAGCACTATAAATAATATTGTCAGCAACAATTTCCATTTTGTATTGCTTATGATTATCTCTATCCACATAAGCATTTCTCTTTATCTTTCCAATAACTTCGACTAAGTCGCCTTTTTTATTGTACTTTTCAAAATTGCTTGCTTGCAAACCAAAAGCTGTAATATTTAGAAAATCAGGTTCCCCACCATATTCATTATTTACAGCAATCACAAACCCTAAAACAGCTTTACCACTTGTTGTTTTAGCTTTAAAGTAGTCTTCACTTACTACTCTACCCGTTAATATAACTTTATTCATCTGCCTCCTTTATTTTGTATTTTTAACTTTTGCTGTTTCACTTTCTTTTTCTACAATACCAGCAACATGTTTTTGAGCTTCTTTAACAGTTTTATCAGTTATTTCAACCTTATTATTTGCATCAATACTTTCTTTATTATTTAAATCAATGATTTCATCTAATTTTAAAGAAGCTAAATTTTCTATATATTCTTTTTTTATGTCATCAATATCTTCTGGATGAAAATTTAAATAATCATCAATATAAACATATTCTGTATCGATTCTACTTAAATCACCAAGTGAATAAATATATTTACTGTTTATTCCATTTTGTTTTAAATCTTCATCTACTAAATCAAATAAATATTCACTTTCTATATAACCATTTTCACCAAAAAAATCATTAAATGAGAATTTTAAATCTAAACTATTATTTTCTATATTTTCTAAATCTTCGACATTGCATATATTTAATAAAGTGATGTATTGCTCCAAATTTTTTTCATTTGTATCTTCAACATAAAACAATTTATCAAAAATCTTTTGATTTATATCATCGTCTACATTAGACATTTTGTCTACTATAGATAATACTTGTTTTTTATATTTATCCCGCATAGTATTATTCTTGATATTTTTTGCTAAATAGTTAAATTTAGCTACAACTTCTTCATCTGAAATATCACTTAATGCATATTGAGTTAGATAATACAAATCCTTAAAACAAAAAACTTTTTCAATCATTCTTTTTCCTTTCTATTTAGCAAAAAGCTTATTGCATATTTTTTTATTGCTTTCTTTTCTAAATTTTTCATATTTTTCTGGTTCCATTTTTTTGAATTCTTCAAGATATTTTTTAGCTAAGGTACTTTTGATACCATTTTGTTTTAAATCTTGATCTATTTTTTGTTTTGATTCTTCACTTAATAAAATTTCATCAAATTTATTGTTTTTAATTTCATGCATAATTTTTCCTTTTCCTTTCTTCTTTTCCATAGTTCCTCCTATTTTTTAGCCTTTGACTTTGGATTTTCAATTTTTGTTTCTTTAGCAATTTCTTTATTCATAGCTGCATTTTGTGCTTTTTCAATATTCGCATTATCATATGCGTCAGCTATAATCTTTCCACTTGCTAAATCCTTTTGGTATTGGCTCATCACAGCCTCTTTAGTTGAAACAACAATTTCTTTCATTGCTTCATTAAACGTTTTTGTTATCTCTTTAGCAACACCATCGCTCAACTTAAAGTTCTTTGCAATTTCCGGCTTTATCTCATTTTTAGCTAAAGCTTTTTCTTTTCTTTTATCAAAGAAATATTTAATAGCAGTTATGCCTATTACTAAAGGCAAACCGCCCACTAAATATGCTTGAAGACATAAGAACCCTTTTAATGCAAGAGCAGGTAATTTCTTCAAAACTTCCTTAAAGTTCTTGTTTCTTATTTCCGCTTTCTTTTCTTTATCAGCTTCTTTATATTCTTTTACCTCTTTTTCTTTTACTTCATTATTAGTAATAAAATTAGTTTTTCCTTCTACTTCTCCTTTAGCATCATATACAACTTCTTCGCCAGTTTTAAATCCATTTTCATACATTGTGCTTTTAACTAGCGCGCCGTCTTTATATTCTTCAACAGCCCCATTTAATAAACCATCTTCAAAATGTGCTTTATAAAGAATATCGCCCTTAGTGTTATAAATTGTTGCTTCACCATTTAATTCATCATTTTGGTAATTAGCAACGCCAACGAGCTGATTATTTTCAGCAATTTTGTAATAGCGTCCACTAAAAGCTCCTTCGTTCATTTCATAGTATTCTTGACTATTATCATTTAAAACATTATGTGCTAAAGTCAAACCACTTTTTGTACTAATTAATTCAAAATTAGTTCCGTTCTTTTCATAGTTTCTACGATTAATAATTTCTTCGTTTTCTAATGCATTAAATACTTGCATATTAAACCTCCTTAAAATAATGTTTAATCTTTTCTACTCTTTCAACTTGCACCCAACCATAATCTCCTTTTTCTATAATTTCATTGGCTATCTTGTCTGCCTCATTAAAGTTAGAGCAAACTGCATATGATTTTGTTTTCTTAGATTCAAGATTCTTTTTGACTCTAATTCTGTAAAAATATCTTTTCATTCGCCTTCTTCTATGTTTTAGCTCCAGCTGTTTTAATATCATTCATCTGGTCTAAACATATATTTATCGACTTCGTGAAATCGTTCACTAGTTCACTACTTAATTTGTTTTTGTATTTTTGTTGGAAACTTAATATCTTTTGTCAAGCAAGTGCTAATTTAGGCCATTCGCTTATCTTGTCTTTATACTCGCTAAGCTCCTTAATACTTCCTCTTGCATTTTGCATTTCCAAAATAAGTTTCTTTTGAACTTTCTTATCATTAAGTTCTTTATTGAGGCTTTCCTCTCTAGCTTTCTTGGCTCTTGAACTATCATTCAATTGGTCATAGTTTTTAATAGGATTTCTAACCGGTAATTCAACATCTGGCAAGTCTTGATAATTTCAAGATTCATTCACAGTGTTTTCTGTTTCGTCACATTCAAAAACATAATTATCATTACTAAAATATTTGTAAGCTAATTTTGTTTTTACTATCATAGGAAGAAAACCAATTTTTTGAATAATGGCATATTCTTTATTCTTCGCTTTAATTTCCGCCACACTCATTAAAGGCTTTTCTTCCACCGATTCACTTTCTGAAGATGAACCACCATTAGCTGATGTTGAAGATGATTTTGAAGTTGATTTTTTAATAACTTCTTTTTTGCCTAAAGAATTAGATAAATCGCTTAAAGTTTTTTCATCAATAGAGTTCAAGAAATAAGTAAATTGCAAATTACTACGAATAATGCTATCCACATTATGATTAGTGCTTGTATTGTATTTTTTAAGCTGATTGTAATCTTGCAATACAAGTTGGAAAAATACATTTCTACTACGCGATATTGTGACCTTATTATCTAGATTCAATATTGGAGGCAGGTTCCCCGCTTCATCTAACATGAAGAAGAACTTCCGTTCAAGTTTCAAGTCTTCCTTACTATTAGCGCAATCAATTAAAGCTTGATATATTTGGTCAATAAGCATACTTACTAAGAAGTGATTACTTGGCTTATGGTCTGGATAGTGAATGAAGATGACATAAGGTTCATTCGAAGATACAATACCATGTAAATCAAAACTCTTTGTTTCTCTTGTTAATCTCTTTATAAACTCATCTTCAGTAAAGTTTTTTAAAGCACTCGTAGTATGTGATAAATTACCCGTTAATGTTTCAGGAACAATATTTGCAATTGTCTTAACATTTTTAAATAATTCAGCTCAATAATACCTTTTATTTGTTTCTGCATTTATATAGCAAATTGAGATTCATTTTTGTTTAGGATTTCAAGTGTCCAAATTACACATTGAAGCTACCGATTGCAAATTGAAATGCCTTTCTGGTAATAAATCCGGCCTATCAATTGAAAGCAACAATAGAAATTGAGCCACAACATTAACAATCAATTTAGCATTATCAACCCACATAGGATTACCATCGCTATTATTCCCTCATTTTAGCTGTTCAACAACATTATTAATTTCGCTTATAGCTTCATTCATGTTGTCCTCTCATTTAGGATGCCCTTCCGGCACTCTATGAGCTAGTTTATAAGCAAATGATAATGGATTCCAACCAACACTTTTCTTTGTATCGCTAAAATCTACCGCATATATTTTGTATCCTTGCTTTTCAAGCTTTTTACCAACCATTTTGATAATTTCTTTTTTTGGATCCGGAATACAGAAATTAGCTCTATATTTATATCTTAGATGTGAATTATAAATAATATTAGGCAATAGTACACGTTGTGTTTTACCTGAACCTACACCCCCCAAAACAACTGAGTTAATATCACTTTCAGGTTCATTCACTCATCATTTATTTAGATTTGAATCGAACTTAATTACCCAATTAGCGTTGTTTTGATTTCCCACATAATTTGGTGCAACTTTAAACTTCTTATCGAAAGTTTCATTACTTACACCGCTATTATCAATTTCATTTCTATATCAATACTTATTAGGTTTTAATAATGATGTTTTAAGTTTATTTTTATAGAATAAATAACTTATACCCGCTATCGATGCAATTAATAAAAATGCGTATGAAATTAAAAACTGTTTTGGAAATGCATAAATGCTTGCTTTTGTCTGTGCAAAAAAGTCTTTAATTGAAACCATCTTAAAACTTTTCAAACGCTTAACATTAAATATAATGGCTAGAGCTAGGAAGATGATAATTGCTGCAAGAAGTATTGCAACAGCACTTAAAGATATTTTTAATATTTTCTTTTTCTTATCTCCCATTATTGACCTCCGCCTTCAGCCATAAAAATATCAAGCTTTTGCACAATAATTCTTTTAGCTAACTTTTGCATTGGAATTGCTTTAGCTATGCAAAGGCATTCTTCAATTATTTGACAATAGCCTTCTACGCTTAAGCCTAAATGTTCAATAAACTTCTGGTCGCATTTATATTTCATTCTTATAGGCTTTATTAATAACATCATGCAAAGGAATACAACCATTAATAATGAAGACGACACCGTAATAGAAATATTTCAAGCAAAACTTATATTTAATACATTCACTAGGCTAGCAAATATGAAGAAAACTAAAATTGATATTGAAAACAATACAAGTGCTACGTTTCTTAATGGCATATTGTTTTTCAAAATCTTTTTATAATGAAGTAAATTGATACCCGTTATAACATCATTCCAATCTTCCGTTTCTATAATTTTTTTAACTATCTTAGATATATTGTTTCCCATAATAAAATCCTATATTTTGTAATAAGTCATAATGTTTTCGTATTCTTGAATGCTTTGAGCTTCAAAAATCTTCTTAGCTCTATTAATCAATCTTCAAGAATCATCATTTACATTCAAGTTATAAAAGAATAATTTTGTAAAGCTTTTAGCACTCTTAGCATTTCTTGATTTTAGAATTGCTTTTATTATCTTGTTCCCTAGCTGTCTAGAAAACTCATCTTTTTCAGTTCTCAAAACATTATTGATTCTCTTTTTTAAAAACGTTGAATTCGTTTCATCCTTTAGTTGCATAACTAAATCAAATGCCTTTTGATAGTTATCCATCTTTCCTTTAAAATCATCATCATATTTATATAAAAATGTTTCTATCTTGTTTATCGCTTCTTTAACTTCTGGTGAAGCAAGTTTATAAGACTTATTGAACATATTCTCCATTTCAGCTCTTACATAACAGCAAGCCTCAATAAAGTCTTTGTAATCTTGGCTTTGAACATTCTCAAGCGTTCGTTCTGTCGAAGATAAAACATCTTTTCTGGCATCTCATAACTTACTTGTTAAATCCTTAAGATTTTGATAATCAAAGTTTTTAATAAGCTTGTTTGTAATCTTCATTCTTAGCATTTTTATATCGTCGATGTTTAATTTTCTTTTTGCTCTTTTTTTAGTCTTTTCAAAAAACATAAAATGGATGTGTGGATTATCTGTATTACCATGTATTGAATAATAAGAACTAAGGTTATCTAAATTAAAACCGCTAATGCTATAGAAGTTTCTAAACTCCTCTTTAACTAACTCAGCTCAATCATTAGCAGTTAAAACATTATGTTCTAAACCTAAATTACCTAAGTTTAAAATGCCTTCATAAAATGTTTGTTCATCAATACTCTTTAATTCTTCCTTAACGTTATCGATATTTACATCAGCACTAGAATCGTTTAGCATATCATAAACACCAGAATATTGGCTACCCATTTTAGACTTAAGATGATTTCTATATCAGTTAATCTTTTCTTCTCTACTTACTTCCTCTAAAAGCATTCTATCTTTTTCGCTATCATCAAAAGGACTAAATGCTACACACTTATCTCCTCTTGCAAAATAATCAATAACTGTCCCACTTGATACTCAACTACTTAAATCATGACAATAGCCTAAACCAGTTTTATAGTTAGTCATTTTAGATACATAGTTGAACTTCATATAAACATCCATTCTCCCTCCTCTTTAACTAACTAGCTCCCTTTAGCTATGCAATGAAACTCAGGAGTAAGTTTTGGTATATTCATAATTTGAGAAAGTCTTTTAGCTAAGTTTTTACGATTTTGTAATCCCAAATAGAGAAAATCATCTCCAACTGATGAAGATAAAAACTTTTGAGCAAACTTATAGTTAACTAAAATTAATTGTCCTTCTTTGTATTTCATTTCTTTCTCCTTCGTATTAATTCATTACATTCATTAATACTCCTAATTATTTATCGACCTAATTTGGACATCATCAGCATACTTATTCTAAAATGTCTTTAAACCCCAAAAAGGTTAAATATGAAAGATAACACACGAGTGTGCGTCCATAAGATTTTCTAAAGCAAGCAGTAATGAATAAACCGCAGTAATAATAATTGAAGCTTATTCATTACTACTCCTCATTTGCTTTATATGCGTATTACAAGATACCTTTGAGTCTTAAAGCTTAAATGCTGCAAGTTATCGACGCTTCTTTGAATAATAAATAACTCATCAGTTCTCAAACCTAAAACCAAACATATATTTTTCAAGTATCTGTAAGCCGTAGAACCTTTTAGCCCTAAAAAGTTATTTTGTACAATGACTTTCATCTTTCTACTTTTAACAAACTTTTTAGTTCCAAGTTCTTTTGATTTTCATACAAGCGTTTTATAAGCTCATAAATTAGCTAATCCAAACAACAAGAAAATCTTCCAGTCTTTATAACCACTAATAACAATGAACTTTCTATCGTCATTAGTTAGCTTAAACTTCTTAATTTTCACAGCGTTTCTAACTTTTAAAATTAGCCCTATATCTGTTAAAAACTTTACCGCTCTTTGCAAACTTGTTTTCTTGAAGCCAAAAGCCACTAATTCATAAAAATTTACATAGCGTTTTTTAGCCCCAAGAAACCTTAAACAGACATACACTTCAAAAGCAACTTTTTCAGTTCTCAAAATCTTATAGTAGCTTTCACCGAGTACTATCTTTGTTTTTAAAATTTCACTAACTGTATCTTGGCTTAACTTGTCATAAACCTTCTTGCCAAAATAATTTTTTCTCCATTCCAAGTTAGCTTCAAACTTTCTAACTTCGTTTTCGCTTCCTTCATTTGCAATTAACCTTAGTTCGCCGTATACACTCATAATTTCTCCTTTTTTTTGAACATATAACAGCGACTGTGGTATAATATATACAACAGGTCGAACGATTGGTGGTGTTCTAGGCGCTGTTATTTTTTTATACTTTTGATCATTTTTTTGAAAGGTATATTAATTTAGCAGAATACGAAACTAAATAAAAATATGAATTAATGTTTATGAGTACTTTTATAAATAATCAAAAGTGAAATAAAAAAAGTGTTCAACACACTTCTTTCTATCACTTCCAATAAATCTTTATTTTCTATTTTATTTTCAATATTGATTTTAAAATTTGTTGCCTTTTGAGCATCTAAATTTATTTTAGATTTTCTTCCTTTTTTCTTTTCCATAGCAAAAGAATTATAACCAAACTTTTTGTATTTTTCTATTACTGAATAGCATGCTGAATAAGAGTTTTCCATTATTTTAGATATTTCTAAAATACTAAATTCTTGAAAATATAATTCGATAATTTTTATCTTTTGATCAAGTTTTAATTTTAAATAACCTTTTATATCTCTATTTCATTTGTTTCTTTTTACTTTTTTAAATTTAAATTGCATAAAAATGAACCCCTTTCCAAAAAGTCCGGAATTTGGGGTTCACTCTAATTTCCTTTTTCTATCAACTATATTTTATATTAAAAAATGTAAATTACAAAGAAAATTGTATGGAAAAAATAGCAAATTTAATTGCCTTTCTATACTAATATTAATATAAATACTAATATGTCTGAATAAGAGTATTTTGAATTTTATTATCATTCAACAATAAAGCTTCTATTGTTTTTTGAGAATATAATGCAAAGCTTTTGTTTAGTTTTCTTTTAATATAAACAGATATGTGTCCTTCTATTAGTGAACTATAATCATTATTAATGATTCCACTTTCAAGTTTAGATGTGTATCTTATCAAACTATTTAAATTGTATCTATCAATATCGCTCAAAACAAAAGAGTCTCTAAAATAGAGATAAATTGTATTTAATATATCTAACAACTTTCCATAATCTCTACTGTTGTAAGCTTCTGATATTTCTTTTTCGATTTTTCACCCACTTAAATTTTGAATGCTATCTATAAAATTGTTAAATCCACGATTTCTCTTTTTGAAAATAAAATCATTAAATTTTCTTAAAAAATGAAATTTACACAAAATAAAATTGCCTTTTAGTTGTTTTGCTAGTGTTTGAATTCAATGAGCACCATCCCCTAAAATATAAACATTAAAGCTATTATAATCTCCATAATTTTTTGATATTGCTTCTTTAATTGTCTTTGATAAACATTTGCCAGAAATCATTTTAGAAGATGGCAAAATATTCATTATTTTATTCAAATTAACTATTTTATTATTCTTCAATTGATATATGGTTAAAAATCTTATTTTTAGTTTTTGTTTTGTTCTTCCTTGATTTGAATTTATAAATGTATCATCGATACCTATAAAAATGTTATTGTTTACTAATTCATTCTTGTAGTTTTTTAGACTGTTTATTTTATCTAAATTTATTTGTTTTACAATTTTATATATTTGAGTTTTGCTTACATTGTATTTGCTCGCAATTCTGGAATAAGTGATTCTATCTTTAACAGCATAATTTTGAATTCTTTCTTTTTCATCTTCACATATAGATGATCCAAAATTTAAATAATCAGGATAAAATGTATGCAACTTATTTGTTGTTTTATCTTTATATCTTTCTACTTTCAATTTAAATGTAAAATTTTCAAATCTCACAATTCTTATAATCACATCATGAGCTTTATATTTACTTTTTCTTTCATCACTATATTTATAATTAATTAATTGGTTAATCATATTTTTAATTAAGTTAATAGCAATGAATTTATTTAATGAATTTTCATCATTCATAATTAATGAACTTAATGAAAAATTCAAATTACTTTCATAATAATTTTGCTTATCTTGTAAATTCATATTTTTATTATAAGCCTGTTTTATAGTATTTTTAGTAAAATCACAGAAATAGAAAAAGAAGCCTATAGGCTTAATCTATAAACGTTTCTTCTTCTTTCTATATCATATTGCAATAACTGTTGCAATAATTCCAGCAACACTTAAAATAATTACTGGAATTAGCCATGAAAGTAATTTTTTCTTGTTTTTAGATTTAATTTCTAAAGCTTCTTTTAACATTTCTTCTGTAAAAATATTAATTAATTTAAATTCTTTATCTCCACTTATTAAATTCGATTCTTCTTTTAATTGAATCATCATTTTTAATTCATTATTGCCAACTTTACTAAAGAAAGAATCTCACTTTTGGTTTTCTAATAATTTAAATTCAATTAATTTGATTATTTCTTTAGCAGGATAATTTTCTTTTTTGAGAATATCAATAATTTTTTCATTTAAAGCTTTTTTGAAATCATTTAAATTCTTTTCACTTAAAGTAATAACTTCAAAATTTAAATCTTTTAAATTTAATTTTTTGTTTGCTGATGAATGAGTATTTATAACATTAACTGTATAAGGATTTTTCAACATAAAATGCTTGCCTTTTAAAGTTAAGGTTGTTCCGATAGACAAATCTAAATTATTGAAAAATGAATCAGTAAAATCAATAGTATATAACTTATCAATTTCAAGCTTTGGAGCTCTCTTTTTAAATTCTTCTATTATTTTTTCTCTAACTTGTCTAATAGCCTCATTTTTAATTTGAGCCATTTTAACAGGGTCTTTTTTATTTTCCATATCAACATTCACTTTTACAGTTAAGCCTGCTTCTGGTCAATTAAAATCGTCATCTGGAGCATTGTTGATAACATGGAGAGTGCAAGGATGATAAGTTTTAAAATTATTGTTGAAGTTTATTGCAAATTTTGCAATATTTTCATCTTTATTTCAATAAATGTCATCGTCTTTTGGATTAATTATTTTGTTTAACTCTTCATCATTGAACGATATATTAATATCATCTATTGTGATTTTTTTGTAAAGAGATTGATCAATTTTTTCATTGTATTTGATTAAATAATTCTTTAATAGTTCTTTAATGTATTTTTTGATTTCTTCTTTATCTTTTAAACCAAAAACATTTAAAGTTCCAGGATTAAAATTCAATAAATAACTATAATCTGATTTAAGTAAACTTACATTGTATGTATATGTATCATTATTAGCTATATAATAGTTTTTGTCTTTTGATATAGCTTTAACTATTATTTGATTTTCATCACTATCTTTCAAAAGTTTTAATTCGATAATATTCAAGTCAATGTCTTTGTTATTTATATCTTTAGCAGATTTAATCAAATTATTAAGATTATTATTAGTTAACTCATTAACAAATACATTAAATGGTATATGTTCTTCAATATTAATACTATTAAATTTAATAAAAATAGGTTTTAATTCTAAATTGATATTAAAGCTTTTTCTTCTGATAAAAAAACTATTTAAGCTAGCATTTTCTTTTAAATCAATAACAACTTTAATTAATTTGCTTTTTGAATCAAAATAGCAGTTTTTAATTGTTAAAAATTCTTTATTTTTTTAGATAACATAATTTTCAATAAATTTAGCTTCATTATCTTTTTGATTTATATAATTTAAAAATTGTTCGTTTGTTTTAAAATCTTGTCTTAATTTGTTAATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTATCAAGATCTTTTTTTGTTATGTTTAAATCAATAATTTTTTTCTTAGCTAAGTATTTTTCGAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTCTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAACTAGGTTTTGTATATGTAGTACTATTATTATCTATTCAAACAATTTTAAATTTATTAATATTTGTTTTATCTAGTGATATGTCATATATTAAATAAAAACCATTTTTAGTTAATTTGATTTCTTTCCCAGGAGCTGAATTAGATATTTCCATATTTTCTGAATCATTTACAAATTCTCATTTTCCTATTGAATTTTGTTTTATTTCTCTTATTAAAATATTTGAATTTGCATTAATAGTTAAATATTGAGTCTTAATAGCTTCTGCTAATACAGATGAGTTATTATTTTTATATTTTAGATAAAATGAACCAAAAGGATTTGAAGCAAAGTTTGAATCAAAATGATTTTTATATTTTCTTAATTGTTTTAAATTTGAATAACTATCTTTGTTTACTGATTCGTCTTGAAGAAAGTCAATTTTATTTATTTTGTCATATAAATCTATTAGCCCATATAGAATTTCGATATTTTTTTCTATTTCTTCATCAGGAACATTTTTACCAAAATAATATCCATCGTAGGGATATGGGCCAAACACCGATTCATCTCAGGTGTGTCATTTATCATATTCATGTTTTAAACCTTCATATCAACTTAAAAATAAATTTCAACCAAATTCTTTATCATTTACAAATTCTCAAAGAAATTTAGATTCTTTTTCTGTTAGTATGGCATAACAATATTTTTCTAAGTTATTTAGCAAAAAATTATTTAATTCATCTATTTTTAAATTTAAGAGTTTTTTTATTGTTTCAGGGGCTAAAATTTTATATCCTAATAAGTCGCTTGAAATATAATCAAAATTATCAAAAGCAATATTATGTAATTTTAAATAATAACTAACTCATTTTTCTAAATTTATTATGTTTTTTCAGTCAATGTAAAAGTCATTTGCATAAAATAAGTCGTTATCTAATTTAAGTTTTGGGTTTCAGTTTAATTCATTAAAATTTAATCACACAATTTCTGATTTTAGTCCTGTTTTAGGATCAATCCCAGGGTCATATTTTTTTGATGGTACAGGGTTCCCATTAGCATCTTTAATTTCATTTCCATCTTTATTTAATTCATTTTTGCTTATAAATTGTTTGTATTCTAATAAATCAGGATTTCAATTTGGATATTTTAAATCAATTGTGTTTGATATATCGCTTTTTTCAAATTTAATTTGAATTTTTTCTTTAATGCCATTTGAATCTTCGTGTATTAAGAAAAGCTTATTAGAAAAATTTAATTTATTTTTAACAATTTTCCTTATATCAATAACACTATAAAAAATTTCATTATTGTTTATTGAAACAATTTTTTCACCATTTTCACTTTCAATTCTAATTTCAAATTTTTCCTGTAAATTTTGTGGTGAAACAAAATAAATTTCTGTTGGTGGATATTCACAAATAATTTCATTTGAAGTTATATTTTTTGATTTAGATTCTTTTATTTGTATTGTTCCATCATTATTTATTTTGTAACTATACCCATAATTTATTATTAATCTTTCTTTTCAATTTAATTGTGAAATATCAGAACTTAGTTTTCAATTTATTTTTTGATTTTTAATCAATAAATATCTACTTGTTTTATTAAGACTAACATTTTCATTAATTAAATAAATATTTAAATATTGATTTTCTACAATTTCAAAATCAAATTTAAAAAATTCATTTTTATTAATGCTTTTGTTTTTCAAATAATTATTTAGTGCTAAATTAATGTCAAGATTTTTTCAATATTGAAGTAATTCTTCTTTAGGAGTTTTTTTATCACTGAATTTATTTAGTTTTAATTTTGAATTTAAATTATCTTTCATGCCTGAATAAGACACCAATTCAATATCTTTTTTGTATGGTAATTTAGATTTTAAAATATCAAATATTTCTTCTCAGTTAGCATTTTTTATAGGACATTTATCTTTAATACTTTCATTTCAATTATTAAAACTATCTTCATTTTTAGAATTAGTGCTTGATATCAAAAATACTCCAGAAGCTGGAATTGTAGATGAAATACCAAGCAAAAAATTTTTTAATTTTTTCATACTATCCTTTCTCCAAAAACACTGTTTTATAGTATTATTGCTTAAATGTTACTTAAATCACCAGATTTGTAAATCATATCTTTTTCGAATTGATTGTAATAAATACTAATTTGAAATCTTGTTTTATTATCTATTGAAAACATTCCATTTCCTATAGGTGCAGATGTTAAAAAATTCTTTTCTCAATTTGTTAATTCATTTTTTGATTTGTACATTTGACTAATAGCATTGACACCATTTTGATTTTGTCTAAAAATTAATGTGTATTGTATGTTTTCAACCATCCCTTTAGTTAAGTTCATAACTTCATCACTTAATTCAAAATCGCTTGGATTTTGTGTAGTTAGAATGACAGAACCATTATATTTTCTTATTGTTTTGACTGTGTCAAATAAAAATTCTAATGATGACATATTATTAGCATTAATAAATCTATGAGCTTCATCTACAATAAGACAAATTTTTCTAGTTTTGTTTTCAAAAAAGTTATTTGATATTCTGTTTCTAACATATGAGAGTATCAAATATAATGATGCTTGTCCCTCATTTGAAGCAATGTTATTAATTAAGTTATAAGTATCTATTACTGTTAAAGTTTTATTAAAATTAATATTTGTATGCCCATTATAAAGATTTGAAAAAATGCCATTATTATTGAAATCTTGATCAAAAATTATTGACAATTTATTTAATGAATCTTTGTAATAAAGCTCTGCTTTTTTGTCTTTAAATTTATATGATTTTAAAAATTTGATATAGTCTTTCATTGTTGGAAAATCTGTGTTTTTTAATTTTTGAATATTTTTTATCTTATTAGATAAATAAAATTCATTTAATAAAAAATTAGCTACTCTTAAATCTCTTGCATCTAAATCTGGATAAAGTAATGAGAAAAATTCTTTAACCATTTTTGTATGATTACTAATTAATAATTCATCACTGACTTTTATATTATCTTTTTCAATTTCATAATTGATTTGTAATGGATTAATTTTTGTTTCTAATCCTTTACCCATTTCTATTCAGTTACCATCTAATTTTTTGCATAAGTCTTTGTATTCTCTTTGAGGGTCAATGATAATAACTTCATTTCCTACTACAAATTGATGCAAAGCTATTTTAGAGGTAGTTGCTGATTTACCTGAACCAGGCACACCTCAAATTATCATTGAAAATGATGTTCTGTCTTTTGTCTTTTTAAATAAGTCAAAAATCACAGGACTACTTGAGGTTGTTGTAAAACCTAATAAAAAGTTATTACCATCATTTAAAGAATTAGAAATTCAAGGTCAACCATATACTATATTTCTTGAACTCATTTCAATTCCATTATTTAGAAAATCATCTTTGATAAAAATTGAATTTTGCAATGCTTGCTTTTGTAAATAATGCATTTTATAAACATTTGCAAATGCTTTTTTAGCATTTATATCATTTTTAGTTTCTAAATCTTTTATTCCCTCTAAGGTTTTACTTTGATTTACAAAAATGATATTAGAATCAAATAAATTCATTTTTTGAACATTAATAAAATCTACTAACTCATCTAGAACTTTTTGTTCTACACTGATTTTTTGCATTTTAAAATTACTTTTAGTTTCAGCGTTTGCTTCATTTAATGACATAGCTTTATCAAGTATTTTTTGTTTAGTGGATTCATTAATAGGTTTTAAGTTTCAAATAACTAAAGATTCACTGTTAAATAAATCACAAGCTCAACCTTCATTAATTTGTAAAGAGTTAAAATCAACTAAAGTTTGAAATGATAAATACTCATTATCAACTTTTATGTATTTGGAATTAAATTCAACTCTTTCTGGTGCTAATAAAGAAATTAATTTGTATTCTTTGTCACTTTGAAGTTTTTTAATTACTTTATCATTTAACTTTTGACCTTGCATTTTCATATCAAGTTCTTTTGTTAATTCAGTATCCAATTCTTCAACAAAATAATTTTGTTTGTCACTTTTTGAAATATTTTTTTTAAAAATCATATTAGCAAAGTCTATTAATTCCATACCAGTAACAACATTAAGTGGTATTTTTGTAGTTTCAAAAGACTTAATAAATTCATTTTCATATTGTTTAAGTTTATTCATTTCATTAGCATAGATAACTAAATAATAGACATCTATGTTTTCAATATTTCTAAGACTTTCAAAATCATTTTGACTATAAATGAAATGTTTCTTTACAACATCATTGGTTTTTACCAAATCAATTGTTTTATAGAGATTTTCATTAATTTTAATATTGTCATCAATGTTAACTGAAGAAGGTTTTTTTACAAAAGTGAATTTTGTTTCAATTGAATTTAAAACATTAATTAGTTGAGAAAAAAACGCCACCTTATCATCCCTATTATTAGAATAAATATTATTACCATAAAACTTAAAAACTGTAAATAATCCTTTTCCTACTGATGAAAATGTTTCAACATATTTATTGTCAATTATTCTTTTAAATGGTATTAAAAGTTTTGTGTCATTTGAACTGTTTAATTTATATTTTTTTACTGAAGATTTAAATTTTAATATTCTTAGTATTAAAACATATAATCTACAGTTATGTTTTTTGCTTGGAATAATTAACCAAATTAAAGAACAAAAAGCCACTAAAAATATAATGATTTTTATTCACAAAGGCAATCCATTAGCAAAGCCTAAAGCTGCTGAAAAACCTGAAATTCCAAGAATAATTATTGCATCATAAATATTTAAATTTTTTCAAATATTTAATTTTAATGATGATATTTTTTTGTTTTGATTCATTTTTATTTTTTCCTTTCCATGCTTGTTATTATTCTTTTAATGGATGATTCATTTTCAATTAACTCACTTAATTCTTTGCTTATTTCGGATGTTTTTTTAAGGTCTTTTTCACCTCGAAGAGTTTTAGACAATTGATCCATTTGTTTTTGTGTACTTGCTAATTCTTTTTTTCAGTCATTTAGAGTTTCGGGTTTTTTAGCTTTTTTTGCGGCACCTATAGTCTGTGGAGTGGTTTCTGGTTTTTTTGTTTGATTATTATTTTGCATTATTATAATCAACTCAGCATTTTTTGCGTTCCCTGTAGGATGATAATCTGTTTGCGAAGTGGTTTCAGGTTCTGAAGATGAATTATTCTTTGAATTAGATTTACGTTTTGAAATTTTTTTTGTTGCTTTTGCAGCTAATTTTTTGGTAAATTTCCCTAGATAACTTGTATATTCAACTCCGAATATGCCAAACATAACTTTATTTAATGATAATGAAGTAATTAATGCACCTGTGTAAACAATCAAGGCACAAATGCCACTATCAATACCTGTTGCATCTGACATAGGCTTAATGATTTTTGATGCGCCACTAAAAAATAAGACCATAAACGAAATTGATAAATTCAAAATCATAATTGCTAACATTTTTGAAAAGAATTGTTGTTTTCAAACTTTTAATTTTTTACCACCATCTTCAATAGAAACAACACTAATTAAAGGAGCTTGAATAAATAAGAAAAAAGCTTGATAAACATTGATACTTATATTGATTGTTATATTGCCAACAATATATAAAGCAAAAATAGCTATGAAGCTATTTAAAAATAAGCTCCCCATATTTGAATTTAATAGACTCAACTCATCTTTCATAAATTCTTCAAAAGTAATCGAAAATGGATCGATAAATCTAGGCTCAGGAGCAACTGATTGATAAATTAAATTACCAAGTTCATTTGTTTCTTGACCACCACTAATTACAGTGTTTAATACATTAATGCCATATTTTGCAAAAAATGTAAATACTCAAATTAAAATTGGTATTACTATTATAAAAACTAAATTAGGTATTATAGCTTTCAAACTTGCTTTAAATGATTGAAAGCTTTCTTTACTTTTATTATAAGAAAGCTTAAAAGCACTAATTGCTAAGATTATAAAAAATAAACAAATGCTAAATATAGCTATGCCAATAAATGCACCAGGCAATACCTTTAAATCTTCATAACTATTAATATTAGAAAGTGGTTTACCAAAAATCATTAAATTAAGAAAATCAAAAGAAACAAAATTAATTAATTTTGATAAACTATTAATAATAAATAAGGGTAAACCAATTAATATAAATCAAGGGGCTTGATATAATTTAAATAAAAGCCATCAAAAAAGGTCTTTAAACATAATTATTTCAATGAATTAGAAATTAAACCACATAGTCCAGGCCCCAAAATTAATAATGCTAAAAGAACACCACAAGAAATTAAAGAACCATTTAGTGCCTTTTTTAGCTTTGCAGGAGTATTGTTGCCTTTTGAAGTAAATGCAGCATTCATAGCAATTGTGATTATTGATGTTACAAATGCAATTGCAAACAAAATTCCAAAAATAATAGCAGAGTATTTTACAAATTCATCAAGTACATAAGTCGATGTATTTTTTAATACTGATATTCCTGGATTATTGTTTGCATAATATAAAGATGTTATTTTCATTTTTTCCTTTCCAAAAGCAAAAAATGCACTATCAGAAATAGTGCTTGTATTTTTCTTGCTTTTTATCAAACTAAATATTTATTTTGTATTTTTTATTTTATTTTCAAGTCTGAGCTTGAAACATCTCCAAATATTTTATAAGTATCTTGGAGTTGTTTAAATTTATTTATTAATTCTTCATCAGTTGCTGATGCATAATTAATAGGTTTATTTTTTTGTTCAAATTTTGTTAATGTTCTGGCATCTAATTTAGCGACGTCATATAAGAAATCTATTTTTTCTCAAATATCATATCCTAGGTTATATTTTACTTCTAATAATTTACTTAAAGGTAAAAGAAATTCTTTTAAATAATACATAACTTCATTAGCAATAAATTTTGCTTGTGAATGATCTCATTCTTCATTTTGAGCTTCAAATAGTCAATATAAAGGAGCATCTTTAGATAATTCTTTAGAACCAAAAAGTTTGTCTGCTTTATGTTGAAAAGCTATATTAGTATATGCTTTTCAATTTCACGATCCAAAACCAGAATCAATAATAGGTAATAATTTATTTAATGCATTTTTTGATTTCTCTAAAGCTCTTAAAACTTCAGGTTTTAAATCTTGCAATTGTTCTTTTAATGTTTTATTAGTATCAATTTTTAAGTTATACAAGTTTTTAAATAAATTAATCATTGATACATATAAGTTGATTTGATTACCTATTGTGTTGTATGGTTTACATAAATTTTCAAAATGCTTTACTCTATAAAAAGCTCTTGTTGCTTCTTCCATCATTAGATAAATTTTATCTAGTGCTTGAATTTGTTTTGAGTTCAATGATGACAATTTTCCATGAGTTGATTCATAGTTAATTGAGTAACCATATTTATTGTTTAATCTATCATAAAATCTAACTGCATTATCTTTAAAGAAATTTATGAATTCATCTTTTGTTAAGTTAGGTCAAGTTTTCTTACTAAAAGAAGTTTCAATGCAAAAGTTAATATCACTTAAAAAAAGATCTTCTTTCTTTCATTTTAAGTCTGGATCTTTATAATTTTCCATAAAATATCCTAAAGCATCTACACATTCAATGTAATTTTTATAAATATATGTTGGACTTTTATGGTTTTTTTCTACTTCTTCTTTGTTGTCTAAAACTTCAAATTTTTTATTTTCAAAATATTTCAATTCATTAATTAATCTTTCTTTTCTAGTGTTAATAGATAAAAGGTCTGTTTCATTTAAGTTTTCTTTTAAATATTTTCCACTAATTAATGTTTTTAAATATATTCCATAGTAGAAGAAACCAGGAACTTGAAAATCTAATCAATTCTTAACATTTGCACATAGTGTTACGTCATGATTAGCTAAGTTTTTATCTCCCATATAAAACTCAATATTTTTAAAATCTTTAATTTCTGGTCTTATAGATTTTTTGGCAGAAATAACTTTTTTATCACTAAAACCTTCTGCATTTTCCTTTTTTATAGTTGGGTTTTTAGATGGTTTTGGTGGATTAATAGGAAAAGCAAACCAATTACAACTAGTTGCTGTTAAAGCAGTTACAATTGGTAATGTTATTGTTGGTAATATTGTCTTTAATCATATCTTTTTAGTTTTCATATTGCTCCTTATTTGCTATATAAATATTATACTTTATATTTATATTAAAATAAATCTTTAATTAAAGATAATCTAAAAATACTATAAAACAGTGTTTTTAGTAAATTAGCTAATTAATTTGTTATATTTAATAAATGGTCATAATGTTGCTTTTATACCTAGTTTTTTGTTATGTATATCTAATGTATGTTTCTTATGCATTTTAGATGCAATAATTCATAAAGAAACAAATCTATAAAAAACAAAAACAGTAATTACAACATTAATGATAGTATAAGTTCACAGCAATGCTTTAGAAGACATTTTTAATAGTAAAACATCTTCTGAATTAATTGCTAGCACTAAAGAAATAGGTAATAATATGATACAAAAAAGAATTAAAGTAAATAGGAATCAAAAATTAACTAATCAAAAAATTCTTTTTTTAGTAAAGTTTGTTATTGGCACTTGTATAGTGCTTTCTAAAGTATAAACTGTGCTTGGAATTTGTTTTGGGATTGTATTATTTTCTTTTGAAAAAACTTCAGAATTTTGATCTTTTTCTTGCATAATATTTATAGATTTTTGCTTATTTTAAAGATAAAATCTTTATCGTTTTTTAAATCTTCACTAACAAATTTCAAGCATTTTTCATTTACACTTACAGCTTTAACCATAAATTTTTTATTAGAATTTAATTCACCACAATATTTTAGTATTCATTCTTTGTTCTTGATTAATCTTAAAATTAAGCTTTGATTCTTTAAAATTTCATCTGGTGCTAATTTAATAACTGCCGGGTGAATATTAAAAGCTTTAAAACAGACATTTAAATCTTTTTTAAGTGAATCAGGTAAATACCTGAAAATATATGGATTTCTTTCTATCAATTTTAAAATCATTTTTTTATTGTTTTTACATTTATCAGAAAAATTATCAAAAATTTTTGTATCTCCTGATTGAATAAAAGAAGCTTTTATAACATCATCATCTTTTTGTAATTCTTGAGATGAATAGTAATAAGCTCTTCCATTATTTTTTACAGCTTTTAACACTATTTCTTTATCATTTTTAAATTCTGGAAGACAATACTCAATTCTCATTCCATCATTTGTGACAGCTTTTAGTAAACTTTCTCTGTCTGTATAATATCTAAATTTTTTCATTTTTTTCCTTTCTTCTTTTGTCAGAATTTTCTTTTCAATATCTTAAAAGTTTTAATCTATTGGAGTTTTTATCATTTGAAAATATATATTGTCTAATTTGTTCTAATTTAGAATCATCTTTAAAAAGGAAATTGTAAAATTTGATTTTGTTTATTTTTTTAAAGATTTCAAAAACATTTTTATCTTTATCAAAATCAATTCCTTTAATAAATAAGTTTTTAAATTCTTTTTTGAAATTAGTTGAATATGTCATTAAATCATTTCAAGATTTTAAAGAATTATATTTTTCAAAAAAATCTTTATCATAATAGCAACTTACTCAATTTTTGTCCATTCCATTGTCTGAATCATATTTTCGAATTAAAAAGTTATCTGGATTTTTATTTTCAAAATCATTTATGAAAACGACAGCACCTTTTAATATTATTTTTTCATCAAAACAGTATTTAATAAGTTCTAATTGCTTTGATAAGCTATAAGCTATTTCATATCTTTTCTTAGGATATTTACCATCACTTTTTCTGATTCATAACACAGTTAAGCAAGAAGTATCACCATTTTTTCTAAAAATAAAATCAAAGTTAATTGGCACTTCTATGTTTTTATATTCCTTGCCTTTTAAAATTGTATTTATATCTTTGGCAGTTGTTTTTTCAATAGTATATTTGTTTTCTTTTAACACATTTTTAATTTCTTCTTTTAAAAACTTGAAGTACTCAACATTTTGAAGTCTATAATATTCTAAATTTATAATTTCTTTACTATTATAATGTTTAGATTTAGATAAAAATTTATTTGGTTTTCTCATAATCAAATCAATTAAACTTTTGACTCTTCTTGATTTAAAAACATCTAATAATTTTTCTTTGAATTTATTGTATGTCATTTTTTATTAACTCCTTCTTTTATGCTTCAATAAGCTTGTCTCCTTTTTTGTTTATTCTTACTAATTTTGGCGCTTTTGATCTTTCGCCATTTGTTAAACTAGTTATTTTTTCAACATATTCAAGTTGCTTTTGTAAATTCAAAAATGGATAACTTTCATCTGTTGCACATTTAACAAAATCTTTTAAATTTTTTACATGTATCTTAAAAATATGACTTAGGTCAATATAAGATGAAATTTCTTTTACATCATCTAATTCATCTTTTGAATTTACTTGATTATTTCTTATGTCAATTAAGTTTTCTTTATCATTTTTTACCTTAGCATTTTTTGGATAACATGCAACAAAAAACAATCATTCATCATCTTCTGCCATAAATAGAATAGAATTAACTTTTAATTTTAGATAATCTTCAATAATTGCTCGACCTTTAATTTTTAATGGTATGGTTGATAACATATTAATTTTATTATTTGGTGTGTTCATATTTTCTCCTTGTTTTTTATAGTTATTAATTTTGTTTTTCTAATATTAGAATTTTTTCAACTTTAGTTGCTTTGCCCTTACGAGAATTAAGTAATTTAGAAGTTTTAAAATTATTTGTATTGGTATCAATTCCTACTTCTAGGAATGAATAAGGAACTTCATTTTTAATTTCTTTTCAAATGTTTTCTATTAATTCAAAATCATAAACATCACCAATTACTAAACAAACTTTAGCTTTTGGTTTTAATTTAGATGAAATATTTATTAAGTATTTTTTTATAAAATTTGTGTATTCTTTTAAGTCAAGCGAATCTGAAAGTTTGATTTCTTTTTTTAAATTATTTTTTTCAAATCCTAATAATCAAAGTCTTAATCAATTGCTTTTTGTGTAATCGACTAAAGATAAATAGGGTGGTGAAGTAATAACTAAATCAACACTATTATTTTTTAAAAAATTTAAACTATTTAATGAATTTCAATATTTAAAAATGGACTCATAATTTTTAGATAGAAAAATTTCTCATTTAATTTTTAATCTTTTAATTAAATTATCGAATATGTCAACATTAGGTTTAGTTAATTTATTTATTTCAGAATATTTTTTAACATAATTTGTAGACATTGAAATAGTATTTGGCATATTTAACGAAAAATAAATTGTTGTACCATCTTTTTTCATTGGACCGTGCATAAGTCCTAATGATAATGCAAGTATCAAATTATCAATATCTTTGTTATTTTTCCAATTAATGCCAATTTCATTTTTTAGAAAAATTAGTTGTTTAAGTGTAAAATCACTATAAAAAACTTTTAAATCATTATATTTTTCACTCGTTATATTTATAGACCTTGGCAAAAGCAAATACTTGTTTTTTAATTCTTTTATTCGTTTTTCAATCTTTTCTATATTTGATATATTTATAAGTTTGGATTTTGACAATACAAATGCATAAGGGTTTAAATCATTACCTACAAATTTTCTATTTAATTTTCTTGATGCATAAGCTGTTGTGCCTCTTCCTGAAAAGTTATCCATAACTGTATCATTTTCCTTGGTATATTTTTTTATAAAATATAACGGTAATTCAACAGGAAACATAGCTAAATAAGAAGATATTGAATTAAAACTATTAGGACTGTATTCCTTGTTTAAATTTGCTTCACAAATAAACATTTTTTTATCTATATCTTTAAAAAACTTAATTGATTTTCTCATATTTTTTGTTTGTTTTATTTAATAATTTTTTTAATTGTTTTAAATTCATTTCTTTATTCTTTATTGCAAAATAATCAATAGCTTTCAAATACACTTCATTTAATATTCCAGGATTATTATTACAATTATTGAGAATATATTCAACAAGACTATTGCCTAATTTAAAATTAGTTTTTAGCCTATTGTAATTAATAATTTGATTAAGATTATTTAAAGAATACTTTTTTAAATTGAATATTTTAAAGCCTTCTCATTTTGAGGAATCAATTTGATTTGAAGAATTTATTGTCCCTATAATCGAAAAATTTTTAATCTTAAAACGCTTGTTTGAATAACATATTTTTTCAACTTTATCTAGATTTATCGAGTTATAAATATCAAATAATAATTTTTTGAGCTCTGGTTTTAATTTATTAATATCTTCAATGAATAAGATTGCATTATTTTTTAAATTTGCTAGTATGTTTAACAGTTCAAATTCATCAACAATATTATTACTAGTTATAGGTTGCAAACTTCTATCCTTAAATTGAGAAATTATATTCGCTAAAGAATATTTGCCATATCCCTTTGGAGCAACAAAAATCATATTTGGAATTTTTTTACTTTCTTGAAGCAATATAATCAATGATTTTATTAAATCTTTTTGTCCTCTAAACTCTTTAAAATTTATTGGTGTTAAATTATACATTTTACTAAAATGACTGTTTTATAGTGTTTTTGGTTTATCAGACATTAATTTAATTGCTTCTTCTAGTGAAAATTCTATGTCTTGATTTAAATTGATTTTTCTTACTACATCATCAATAAGAGAGTCTTTAAAACCTAACATTTTCAAAGTTTCATTGATTTCTTTTGATGATTTATATGTTGTTACATAATTTTCAAACATAGTTTTAACTTTTTAACGTCTTTTGATTTTGATGATAATTTTTCTTTTAATTCTTCAACAATTATTCTTGCAATTCTTGGATTAATATATTCAATTTTTGCAATCTCTTCAATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAATCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAAGTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAACACATTTTTAATTCTTGATTTAATTTAATACTTTTGTTATCTGGAAACGTAACATAGTATCCAACATTATCTGATTCAAAAATTAGATTTTTTCCATCTTTGTAAACTATTTTTCCTTTTCTATATAAAACCATATTTTTTTCCTTTCTAATCAAAACTATCTAAGTAGTTTTCATATTCAGACAATATATTTATAAAAATTCTTTGTATTTTGAATGAAATATTTTCAGGAGATTTTTTGAAGCCATAACAATATTTTTCATAAATGTAATCCTGATATTCTTTTTCTTCAATCTGATTATTTCCAAGTAATTTAAAAATTTTTTTAATTTTTTCCATTAATTCATTTTTAATTCTTTGGAATTTGAAATTCTTTTTAAAATCATTACTTTTATTCAATTTAAATGCTTTTAGTAATTCATAATATATATTTAGTTGAAGTAAATTATTTTCTAATCTAAGAGTTTTTAATAATTCATAATCAAATAAAACCTGATTGATTTTTAATAAATTAATATCAGATATACCAAATCCAAAATATTTATTAATACTTTGCAACTTTGCAATGTCAAAATTGATTATTGTTTTTGAATCTATTAATTTGCTAATTGATTCCATATTTATCTAACAAGCTCTTTTTTAAATAATTCTTGTCAGTTTTTATAATTTGTTTTTCTTTTAATAGATTCATTAATTAATTTTTCAACTTTAAAATCTTTGGTATCTAGTAAATAAGTTTCTCTTCTATTGAATACACCAGCTTTTAGTAATCAATCTATTACTAATTCCATTTTTAAAGTTCTAGATTTTATATTATTAATTTCTTTGTATTCTTTTTTAGTCATATTTTCTCCTTTGTACTTATGCAGTAAATAAACAATTTAATAATTTTTCACTTGATTCTTGATTAATTTTGCTAGGCAATGTTTGAAATGGAAATTGTGTGATATACAACTCTTTTTTATAATTAATTTTTTTGTTAAGATGATTAAATCCACAATCTAGTATGTAAATGATTTTAGATTTACCTTTACTATTTAATGATGTGAATAAAATTTTTTTATTTTCAAAATCTCATTCTATGAAAACTTCATTTTTGTTTTGAGTTTCCAGTAACTTCAAAATCTTAATATTGCCTTTATAAAAAAGAACATATGGTGAATAGCGATAATTTTTTAATATTTCTGGAAAATTATCATCAAAAGCTGTTATATACTTAATGCCTTTTTGTTTTAGTTCATTTAATACTTCATCTATTTTAGCTTGAGCAACTGTTTTGTAAGTTTTTAAAAATTCATAAATTTTTCAAATATTTCCTTCATATTTTCAACTTAAATAAATTATTAATTCTTTCATAATTAATAAGATTTGTTCCTTTTTGCAATTGCTTTGAATAAATTAGAATTTTTATCATTTAAATTGAAAATATTTTCTTTAATATTTCTATAAATTTCTTTATTTGAATTTAATTTGGTTCATTCTGACTTTTCCAAATAAAGCAATGCTCTAAGTGCTTCATCTGATTGATCTAAATCTGGCATTTTCGAAAAATAATTTTGATTGTTTTCTTTAAATTTTTTTATAAAACTTTCTATCTTTAATCAATCTTCTTTTTTATCTAATATTTCAAAGAAATCAGCTCCATAATAAGGTCTTAAGTCTACTTGTTCATAATAAAAATTATTAAATAATCTAATATTCTTTATATAATCATTATCTAAAAATCATAAAGCACCCTTAATATTCATTTTTGGGTAAATAATTTTTAAGGCTTCTAATTGTTTAATGATATTCTCACAAGTTTTTTTCATTGCTTTATGTGAATAGCAATCTCTCTTTCTTAAATAAACAACAAACATATCTGTTGGTTCATCTTTTGTTGAATTATTATTCAATCTAAAAATGAAAGATGCATTGATTATTACTTGTTCAGGAATGTCTCCTAAGTGTTGTCCCTCTTTAAGAGGCAAATTGTATCTAAAATTACTTCTTTGTAAACTAAAACCATATTGAGTAATTAAACGTTTAGCACAATATTGAATAAATTTTTCATAAATTCTTTCTTGATGCTTCAAAAATGATTGTTTAATTTTAGTTTTAACATCAAATGGGTTGGTTGGTGAAACAAAATTAAAAGGTTTATTGATTATTTCTTTTATAAGTTTTGATGTGGCATCATTTGTTAAGGCTCTTTTAAGTTCTGTTTCAAATTGACTATATTCCATATTTTTCTCCTATTGTTTTATTTTTTATAAATCTGATAACTAGCATAAACTATCGGCGTTGCTGTATAGGTTCCCATCATAGTACCTGTTATTGCTACTAAAACTAAACAACAAGCACCATTTTGATTATCAAGTGGTTTTTTAGGTTATTTTTTAGAGAGTTTTTGAAGGTAGTTCTCTGACAATTTAGCTTAAAAATTCCACTTTAGTTTGGGATTTTGCTCCATGCTTGCTTGCTCGGTCCATTTTTGATTACCTTGCCGAGCTGCTTGGCTAATGAGAGAGCAGAGAGAAATTGATTTATACTTGTTCAAGAGGTCTCCTGATGCTTTTTATTTCTTCATAAATCTGATAACAAGCATAAACTCTGTAAGAATTCGAAAAATTCCATAAATCTTGCTTAAATTTTGAATCATTTATATGTTTTAAATTGATAAAAAAATATTTTTCTGATGATTTAGCAAACACAAAATCATAAATATTCTTAAAATTCTTAATTTCTGATCAAGATTTGTTAGATTTTTTGCAAATTTTTAAATAAATTCTCGTAATTTGTGATAATTTAAACTTTTTCATAGTTAAATAGATTACTTTATGAATCAATGATTCATATATTAAGACTTTTCCATTACATTCCAAAGTCTTAATAACTTATTCCTTCAATTTTGAATAGACAAATATCAAAAGTTAAATAATGTCAAGTGTTTTTTTCAAAATGCAACATTAAAAAACAGATATTTAAAATATCAGTGAATTTTTGTATAACCTACTTTTTATATAATTTTCAGTCATTTTGATAAACAACTGCCTTACATTCCAAAGTCATTTTTGTAATAAGTTATATTCTCATAACTAGCCAACGAAAGCATTTAAAAGAATGTGAATTAAGGTCATATTCTCTAACATATTCGAAAGCTTGTAAATCTGAGCTAATTACATCAATTCCAAGAACAGCAACAACTGATTTAAAAAAGACATAGCCTTGATTTTTTGACAAATTTAAAAACTTATTATTAATTAAAACTTTATTATCAGTATTTACTTCATATTTGTTTTTAGACACTTTTTTAGACTTATATGTTAAAAACAAAAATTTAGGTATAAATTTCAATGATGAGATTAAAGATAATCTTCAAACCTCTTCATTTTTTAAGTAATAATATTGTTCTTTTTTAGTTTTTCAATTTTTATAAGTTAATTTTACAAAAGTAAAATTTGTATATCAAGAATCGTTTTTGTTGATGGTTTTAATCAATTTACTTTTAAGCAATTTTTTGTATGCAAAAAATAATGAACTTCTAGCAAAACCATATTTCATTAATCCTGTTAATTCTACAAATTTTCTTCTAGCTCCTGAATATATCAAAAAAATATAAACATAAAAACATACCTTTGAATTCATTGATACTAATTCATCAAAAATTCTTTTTGATATTGTTATTTTCATTTTGAATAGAGTAGATAAAAAAATCAATGCCTTATCAGCAAAATTTAAATACTTTTTACTCTTTCACATTTTTTCAAATAAAGATTTTGAACTAATCTTTTTGAACAAAGATTCAGTTTTAGTTTCAGAAATTTTAGAATTTGCCGTTTTCTCTGGTAATAATTTTTTATTAATTATTATTTGTTTTTTTTCTGATTCTAACTTGTCATAATTTAATCCATAGTTTTGAAAGAATGTTGAAAAAGTATCAAAATTTCCTGTATAAGACATAATTTCACCTCCTTTCTATATTTAGTTTGATAGAAAGAAGAAAAAAACAAGCTTTATGCTTGTTCTCTCATTTAAATTCATTCCCACAATTAGTTTACAGCACCGACCTGTTTTTTAAATTAATCTGTTTTATAGTTGTTTTGGTAAAATACCAGTTTATTCAAGTTTTGTTCCATCAGCTAAATCTATGGCTACAAAAATGTCTTTAATTTTACTTTTGCCAATTGTATATCTAGTTATTTCTTGTAAAACATTAACAGCTTCTTGACATCTTCTTTTAGCATCTTCAGCATAGTTAAAACCAGCATTTAAAAAGTTGTCATCTTGGATTTTGTTAATAAAATTAGTAAAATCATTTTTTGCTTTTTCTCATATTTCTGCTAATTTTTTATGACCGCTTTTTTTAAGTTCTTCTTCAATAACTAGAACATCTTTTTCAAAGTTTTCTTTAATTTCTTTTCTTAAAGTTAATATGACATTGTATAACTCATAGATCATATTTCCAATTGCATGTGAGTGATTATGACCACTGTTATTACTTGAACTACTATAATTTGAAACTAATTCTTGATTGCCTTTTTCTTTTCAAGCTTTTGGATGTAAATTAGCAATAATATTTGAAATTGATTTATAGTCCGTACTCAAAACAGTTTCTCAGTCTTCATTTAAAATTTGTTTATTTTTTTCAAAATATTTATGCTTAAGAATATAGAATCTACTTCTTTGTTGTTGATTTTCATCGTCTTCTGTTAAACCATCTAATAATCTTGAATCATTGAATATTTGAGCGTTAACATCTAAAGCTAAATAAAGCATTTTATCGAAGAATTGGTAAAATTTGTCTTTTCCACTTTGACTTGTAAAAAAGCTCAATTTGTCTTTAAAATTAACTGCTGATTCAACTTCTTTCATTAAAGTATTTAATTCAACATTATCACTATTTATTATTCCGGTTTGACTGCTGCCAATTATCATATCCATCATGTCACATATTTGAGCTATGTTTTCTTGATTTTTCGATAATGTCATATCTAATGATTTGTCATACAAAACATTATTGTCATATTCAAAAGACAAATTAATTCTAGCTTTATTGATTAAGTCTAAATATTTTTCGATTTTATCGACAACTGGATTATTATGAATATTTTTTTTTGATCATTTATTTGTTGAATTTAATGTGCAACCCGCTGAAATTATTAAGTTTGGTGCTATTATTGTAGGCAACAAATAAAGTAATATTTTTTTTCTTTTGATGAATGATTTATTCATAAAAACTCATCTCTACACTAAAAATTAGTATTTTTTATACATTTATTATATATTAATCACTCAAAAAAACGCAAAAAAGAAATTTTTGCCTTTGAAGGTGTTAAAATATAGTTGCAGCCTTGAATAAGCTGTCTACACAAGCACAGGTTCGAATTTTATAAAAAAATTAGAAATGAGCTGTGTATTATAATTCGATTTAATTTTTCTTTTTTCCCTTAAAGAAAAATTGAAACAAGCGTGCGGAGTATGCTATTTAATTTACAAAATTATTTATCATTTTTTTAATACCCTTTCTATTCTATTTTTTCATCACATTTACTTACCTTTATTTTTTCTGTCTATTTTTTTATGTAAAGGTTAAAAATTTAAAACACGCTTCTCTGCACTTTAGTGTTTTAAACAAAATATTATATTAATAATAATGCAGGTTAACGCCTGTTTTTTTATTATATAATTTAACAAATTAATAAATAAGGAGAGCTATGAATGCTTTAGATATTAAAAAGAAAATTATTGAATTAAGTAATAAATTAAACCAATGAAATAAAGAATATTATCAAGATAATAATCCTAGTGTTAGCGATTTTGAATATGATAAAGCTTTACAAGAATTAGAACAATTAGAAAAAAAATATCCTCAATTTATTTATGTTAATAGTCCTACTCAAAAATTAGGATCTTTTGCTGATAATAAATTTAAAAAAGTAGTTCATCAAAAACCAATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGAAATAAAATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAGTTATTATTTTCATAATATTTAAAATAATTTTTGAGTATTTAACTAAAATACTTTGAGTTTTTATATAATTTAATCAATTATTTAGGAGTTATTATGGATATAAGAAAATATGTTGTTATTGGTAACTGAAAAATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGAGCTATTGGAACAGGTAAAGTTGCAACTCCTGAAATTGCTCAAGATGTTTGCCAATACATTCACTCAATTACAAGTAAAGAATTAGTTGTTCAATATGGCGGTAGTGTAAATCCTAAAAATATTAGCGATTTAGCAGCACAAAAAGACATCAACGGATTCTTAGTTGGTGGTGCAAGTTTAAATGTTGATTCATTTATTAAACTTATTACTTTAGGCAAATAATTTAGATTAAAAACAGCCCTCTTTGCAAGGACTTTTTTAATTGCGATTTTGTATTAAAACAACTGTTTTATAGTGTTATTAGTGAAATTATTAAATTTTATTTTTGATTAATTTCGCTGATAATTCATCAACAATAATTGTTACATCTTTATGATTTTGTAAAAAGCTGGCTGGACAATTTGAATTTACTGGTTTAAGTAAAGTATCATAAATTGCTTGAGCTTTTTTAGGCCCGGTTGCTAATAACATAATTTTTTTACTTTTTAAAATAGTTCCTATTCCCATAGTTACTGCTTTACGAGGAACATCTTTTTCACTAGCAAAAAATCTTTTGTTTGCATTAATTGTGCTTTCAGTTAAATCAACTACTCTTGTAATATCTTGTTCTTGACTATCTGGTTCATTAAATGCAATATGACCGTTTTCGCCAATACCTAAAATAGTAAAATCAATACCTCCAAGTTTTTCAATTACTTGTTCATAGCTACCATTTTGATGTTCTTTTTCATATTTTATGACATCAGGAAAATTGATATTTTCTCTTTTAACATTAATGTGATTAAACAAATTATGATCCATAAAATAATGATAAGAATGTTCATTTTTTAAATCTAATCCAAGGTATTCATCCAAGTTAAAAGTAACCATTTTACTAAAATCAATTTTACCTTTTTGATAATCTTCAATTAATAATTTATATAAATCTAAAGGTGTTGAACCTGTGGCAAAGCAAATTCTTGAATCTGCTTTTTTAGTAATTTGATTCTTAATTAAACTTAATGCTTTTTGTGCTACTGCTAAAGCATCTTTTTCAATATATACTTTCATTTTTACTCCTTATTATTTGACTTTTTGCCCATCAATATAAACTTCTTTAATATCTAAAGTTTTTAAATCCATTAAAACTAAATCTGCTAATTTATTTGCTTTAATTTGTGCTCGATCTTTTAAATTATGAGCTTTAGCTGCATTATATGAAGTTAATTTAACAATATCACGATAATCTAAATCTAATGAAGCTAAATTTTTAAATGAATCATGTAAGCAAATTCCACTTCCAGCTATAGTTGTTGTGCCCTCTAAATAAATTGTTAAACCATGTTTTTCAATATTAATTCCACCTGAAATGCTTTTACCATCAGGTCCATAAGCAGGTCGAATTGCATCGCTAACACACATTAAGTGGTTTATATCTTTATGATTAAGCGTAAAAACTATACTTTCTTGACAAATGTGATGAAAATCAACAATTAATTCACTATAAACATCATGTCTTTCTAATGAAGCTTCTAAGATTCCCGGTTTTCTTGAATCAATTCCACTCATAGCATTTCATAAGTGACAAGTATTAGTTGAACCCAAATCATAGTATTTCATAGCTTGATCATAGCTTGCAGCTGAGTGACCAATTGAAGGCATAATGTCATTTTGAACCATAAAAGGAACTAATTTTTCTTCAACCATTAAAGGATCATAACTAATTTTTTTCAATTGATGATTTGAACTATTTAGCATTTCTAAAATATCTTCTCTAGTTGCTCTTCTTAATCATTCTAATTTGTGAGCTCCTTTTTTAGCCTCTCCAATAAAAGGACCTTCAATATGAATACCAATGTTTTTAGCACCTTGACATTTTGCTAAAGCAATATTTTTTAAAGCAATCTGAATATTTTCTCAACTTGCTGTCATTGCTGTAGGCATAAAACTAGTAATTCCATTTCTAGCTAAATTTAAACTAATATCTTCAACTGCTGCACTACTATCCATTACATCCTTATTCATAAAACCATGAATATGAGTGTCAATAAAGCCTGGAACTAATAAATATTGAGCCTTATTTTTAGTTGGAGTTATTTTAGTAATTTTTTTACTAAACTCAATGTCAGCACACTCAATAATTTGGTTGTGATTAACTATTTTAACGTTTTTTATTAGCATTTTTACCTCATTTGTTTTTTAATAATTTAATTATATTACTTAACTTTTTTTGCCTAAATTAAAAGCAAAAAATGGTAAATAAATTACACTTATTTGCTTTATTTTTGAAAAACCTTTTCAATAAGGTTTCATTTTCACAAATTTTTATTTTTTCCTCTATTTATATGTACATAAAGCATTTAATTGTTTATAATATAAACATATCTTTTAAATAATTGAGGAGTTATTATTTATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGAGCTGAATTAGGCGATGTATTTATTAATGATGCTTTTGGTACAGCTCACAGAGCACATGCTTCTAATGTTGGTATTGCATCAAACATTAAAGAAAGTGCTATTGGTTACTTAATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGAAATGGACCTATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTTCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAGTTTAAAAATAATAAAAAATGCGTAAAGCTCTGTTTTATAGGCTTAAACGCATTTTTACTTTTCTAAATATTCATTCATTTCAAGAGCTAACTTACGTGCATAATCACCAATAACTAATGAAATTTTATTAGTGCTAATCATAATTCCTGTAATGAATTTTGTATCTTGCAATTTATCCATTTTTATTAATGTTTTATCTTTGATTAAAACTTTAATATTTGAAATGCTTGCATTTACTTCTTCTATGTTTTCTTTGCCACCAAGGTTGTCTACTAATTCAACAACTTTGATATTTGAAGGTAGTTTAATATTAACTCCTTCTTTTTTAATCTTTTGTTCTTTTTTATTTTTTAGGTGTCAATATAATCAACAAAAACCTAAAGTTACAATGGTTAAAAGAACAACTAAAAATTTATCTTTTTTACTCATATTTTTATTATACAAAATAAAATTTAAAAGCAAATTAATAACTTTGAACTAAATTTAATTTATTACATTTCTCTTTATCTTTTACAAAGATAATAAAATTGTTGTAAAATAAAAAATAGTTATATATGCTTAAATAAAAAAAGAGGTAGTTATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGACTTATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAAAATGGCAAAAGATAAAAAAATATTACAAAAGTGTTAAACCTGAAGAAATTTATATCTGTGGAATAGACACAGATTGTTGTGTTCAAGCAACAGCAGTAAACTTATTTGAAAAAAGGATTAGACCAATAGTTTTAAGCAATTATTGTGCTTCAAATGGCGGTCCTGAATCTCATGAAGCAGCTCTTAAAACATTATCAAGAGCAATAGGCAAAGAAAACATTATTCAACTATAAAACAGTTGTTTTAGAAAAAAGAAGGTATATATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGAAGAGGTAACAGTGACAATATCCAATTAACAAGAATTTATGGAACAAGTTGAAGCAAAAAAGAAGAATTGGATAACTATTTAGCAATATTAGCTGATAGAAAAGAAAGAGACCACAGAAAGCTTGGTAAAGAAATGAAAATTTTTGCTATGCATCCTTTATGCGGCCAAGGTTTTCCAATTTGACTTGAAGATGGAATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGAAATAGCGCTGAAAAAATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGATTAGCGCCAAAACAAATTACAATTATCCCTGTTAATTTAAATGAAAATTTAGATTACTCTAGAAATATTTTCAATAGATTATGAAATTGTAATTTCAGAGTCAAGATTGATGATCGTGATGAAAGATTAAATAAAAAAATTCGTGAGGCTCAAATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAGTAAACAAAAGAAAAAAGAAGTTAAAACATGAAGACCATTAGTCAATGTATTATTTACATTGCTCTTTTGTGTTGTTTTTCCTTTTGTTTGATGATTATTTGCAACTGTTGATTATCATAATCAAAAAATTACAAATTTAGCAATTTGTATTTCGGTAATTGTTATCTATTCACTTTTAATAATAGGATTAAATATTTTGCTTCATTATTTTAAAGTTTTAAATATTAGAAGTTATAACTTTAATATTCCAATTCTTTCAGTTTTCTTGTGAATTATTTTAACTTCATACATATCTAAATTTAATATTTATGCTCGTGTTGGAGTAGCTATTGCAGTAGTAGTTGTAATTACTTTAATAGTTAACTTTACAACAGGTAAAATTGAAGATAATATGGAGAAAAAAGCTAAACAATTAGAAGAAGAAAAACTTAAAAAAGAAATTGAGGATTTGAACAAATAATACTATAAAACAGTACTATAGGTAAATTCTAATAAAAAAATTATCAATAAATAAAACTAATTTATAATATTATTAATTAGTAGAGGAGTTTAGTAATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAATTATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAAAAGTGGGATTTCTTTTTATTTAATTAAAAAGCAAAATAAAAATAGCTTGTTAGGCTATTTACTATTTTTTGATTGTGTTTGATTATTTTCCTGATTATTATCTTTATTTTGTTCTTTTTCACCTTCAAAAGTTAAATCACTTTCTAAAGGCTTGTTGACAATTTCTTCTTTGACTGATATTTCAAGTCCAAAAGGAACTAATAATTGTAAAGCTAAGTATATTAAATAAAAGTCATCCATTAATTTATTTGTTAAGGTTTCAATAATATCTTCTTTATTAATGGTTTTGGCTTCAAAGATTAAATCAATTAATTCAAAAGGAATGTCAAATCAAATAAATGCTTCTGTATTTCTAATTAAGAATGATAAATTATTTAATAAATATTTTTTATTCGCTTTAGTGTTAGGAACACTAATTTGGCTAATAGTCAATTCATTGAAAATTTCATTTAAGAATTCTAAATTATTTTTTTGATCTAATCAAGCTTTTAAAACAGCATGATTATTTGATTTAAGTAAAAGTTTTTCGTAAGCTTTTGGTAATTCAACGCTTGTGTAATCACTTGGTGTATAGAAGTTATCTTTATCAAAGAATGACACATTTACTTCGGGATTTAGTTCTCTAAGTTTAACGGCAATTCCGAAGCGTTTAACACTTAGTTTTTGTTCGATTAATTGTCCATATGTTCTGCTTTTAGCTTTAATTAATTGTTTCTTAAGTTTATGATTAATTGAGGCTAAATCATGTCAAATGTTGCCTTCGTAGTAAACATTTTGCATCTTTAAATATTGGTTATCAGTTAAAGCTTCATATTGTTCATATTTTTTATGGATTGGAGCAAAACCAAATTCAAAATTTTTGAAATTATGTGTGTTTTTATAAAAATTGATTAAGTCATAATTTTTGTGAATTTTGTTATTTTCATCAACGATATTTTCTTTGTAACCAAGAGTAAAGAAGTTAAATAATTTTTTGATTAATTTATATTTATCAAATTTGGTAATAACATCTTTCAGCACATATTTTTTAGCTTTTTGTCCTATTAAAAGAACAATTAAGCCAGGGATAGTTAAAGTTAATGAAATACCAAATAGCATGCCAAAGAATCATTCACTATTGTTTTCGAAAAATGTTTCTTTTTTGCGAAAGTAAAGAGCAAAAAGGGCAATGGTTGTTATTAATAATAAAAGACCTAATAAAAATATTCCAACGCCACTTCATAAACCGCCTTTAGCTCAATTAATCCTTTTGCTTTTCTTTTTATCAGCATAATAATTATTAATTAATTGAGTAGTTAATTTGTTTATTGTTGGACGTTTAGAACGAATAAAAGCTTCTTTTGAAATTAACTTATTTGATACTGACATTTGACCTCATTTCTATACGTATTATTATAATATTAATTATTTAATTTATTTATTATTTTGTTGTTTTTAGTATAAAAAACTTTTGCCTAAGCAAAAGTCAAAAATGGTAATTTTATTAATGTATATTTAGTACCCTTCTTTTGAAGATTTACCTTCAACAATGGCAACTGAACGGCTTGTTCCGAAACGGTTTGCGCCAGCTTGAGCCATGTTGATCATGTCATCTTTGTTGCTGATTCCACCAGCAGCTTTGATTAATAATTTGTCTCCACAAACACTTTTCATGATTTTAACATCTTCAAGTGTTGCGCCTCTTGTTGAGAAACCTGTTGAAGTTTTAATGAATTCAGCATCAGAAGCCATAACGATTTCAGTAGCTTTTCTAATTTCAGCTTCTGTTAATAAAGCTGTTTCAATAATAACTTTTAAAACATGTTTGCCGCATGCTTTTTTAACCGCTTTAATGTCTTCTAAAACATAGTCATAGTCACCCATTTTCATTTTTCCAATTTGAATAACCATGTCAATTTCATCAGCACCAGCTTTAATAGCTTCTTTAGCTTCAAATGCCTTAGCACTTGTAAGCATAGCACCTAAAGGGAAACCAATAACTGATGTAATTCCAATGTTTGTTTTAGTTAATTTTTCTTTGCAGTATGCAACTCATGATGAATTAACACAAACAGTTTTGAAGTCATATTTCTTGGCTTCATCTAATAAATTATCAATTTCTTTATTTGTCGCATCAGCTTTTAAATAAGTGTGATCAATCATTTTGTTGTAATTAATTTGCATAATTTTTTCTCCTAATTTTTTATTATTTCAACTTAGCAATTATAATCTTATTTTCAACTTTTGAATTGCCAATTTTGTAACCTGTTTCTAATTCTTTAACTAAATTCTTGTCAATCGGATTTGAAGAATAAAGAGTAAATAGTTTTTGCCCTTTAGTTACTTTATGATTTGTTTTAGCGTGAAGAGTAATTCCTGCTTCAAAGTCAATACTATCTTCTTTTTTAGCACGGCCGGCGCCAAGTTTCATAGCTATAATACCGAAAACTAATGAGTCAAAAATATCTAAGTAACCATCTTTAGCAGCAACAATATCTAAAGAATGTTTTGGACTTCAAAATTTAGGATCTTGTAATGCTTTAACATCACCACCTTGAAGTTTGACAAATTCATAGAATTTTTCAAGGGCTTTGCCATTTTTAATAACTTCTTCAACTTTTTTAAGCCCTTCAGCATGAGACTTAACTAATTTGGCTTGTTCTAAAATAGTTGCGCATGAAGAATAAACTAATTCATTGAAATCTTCTGGACCTTTGCCTTGAAGAGTTCAAATAGCTTCTAAAACTTCATTTTTGTTTCCAATTTCACAACCAATTGGACGGTTCATGTTGGTAATTTCTGCACGAACATCAACGTTTAATTCTTTACCAATTGAAATCATAGTTTTAGCTAATTCTTTAGCTTGGTCTTCATTTTTCATGAAGGCGCCTTTACCGCATTTAACATCTAATAAAATAGCATTTGAACCTGTAGCTAATTTTTTAGACATAATACTTGAAGCAATAAGAGGAATTGAGTTAACACAAGCAGTAACATCTCTTAAAGCATAAAGTTTTTTATCAGCTGGTACTAATTCTTTTGATTGACCAATAACTGAAATATTATGTTTTTTAACTTGTTCAATAAATTGACGTTCAGTTAATTCAACATGAAATCCTGGAATAGATTCAAGTTTATCAATTGTTCCACCAGTGTGGCCTAAACCTCTACCACTCATTTTCGCTACTGGAGCACCTAAAGCGGCACAAATAGGAGCAACTGCTAAAGTAGTTTTATCTCCAACTCCACCTGTTGAGTGTTTATCAACTTTAATCCCTGGAATTTCACTTAAATCAATAACTTCACCTGAGTGCATCATGGTTTTAGTAAAAGTTGCTAATTCTTTTTTTGCCATTCCTTGGAACATAACTGCCATTATAAAAGCAGCCATTTGGTAATCAGGTGTATTACCTGATACATAAGAAGTAATTAAGAATTCAATTTCTTGAGTTGTTAATTCTTTACCTTCTCTTTTCTTTTCGATTAAATCAACGACACGCATTATTTAACCTCCCTTATTTTGCAACACCTAAAGCAATTTCCATCATGTTTTTGAAAGCTGTTTGTCTTTCTTCGGGTGTTGTTTCGTCATTTGTAATTAAGTTGTCACTAACTGTTAAAAGACATGCTGCATTTTTACCTAATTTTTCAGCATTAGTAAATAAAGCATATGATTCCATTTCAACACAAAGCGCATGTGTTTCATCGATTCTTTGTTGAAGAGGAACTGATGAATAGAATACATCTGAAGAGTGTACACGGCCTAAAGTTAATTTAATGTTCAAATTTTTAGCCACTTTTTCAATTTCTTTATTTAATTTAGCACTAGGTAAAGCTACATGGCTTTTGTCTCCTAAAACTAAACGACGATAACTAGCACCATCTGCATAAGCTTCAGTTGCTAAAATCGTTTCATATAGTTTGATTTCTTTTTTGTATGAACCAGTTGTACCGATTCTAATAATGTTATCTACATTGTAGAATTTGAATAATTCATATGAATAGATCCCAATTGATGGACAACCCATTCCACTTCCAGCGATTGTTACTGGACGACCTTTATATGTACCTGTATACATGTACATGTTTCTGACAGTATTAACAAGTCTATATCCTTTGTCTAAAAATGTTTCAGCAATAAATTTAGCTCTTAAAGGATCTCCTGGCATTAAAACTGTCTTAGCAATATCACCTTCTTTGGCATTAATGTGTGGTGTCATAAAATCTCCTTAAATTAATGTTAAATAAGTACTTTCAAGTTTGGTTTATATTTAATTTCAAGTACATTATTGGACATCAACTAATAGAAATCTGATAAAGTGCGATTTAAGTAGTATTAGCGATTTAGCTTAATATTTTCTATATTAAACATCCGGTTATAATTTTAACATATATTAAATTTAAAATATATAAATATCACTATTAGAGCTATTTATTTGATTTTTTTAAGCTAAAGGTGTCATTTTTTCATATTTTAGTTGCCTAAAAAATACAAAAATAAAAATATTTTCATATTTGTTAATTATCAGGCTAAATAATATTTTTATTTAGAAACTACTTTTTATATAATTAGTTAGATATTTAGATACTTTTTTTGTCGAGGTGAATTATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGAGAAACTAGACCTAATGTTTTAGGACACATTCAACGTGGTGGTAATCCTTCAGCTCAAGAAAGAATTCTAGCTTCACTTTTTGGAATTAAAGCTGTTGAATGTTTACTTGAAGGTAAAAGCGGTGTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCTCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAAATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAATCAATTATAATGTCATTTATTTGGCATTATTTTAATTTTTTAAGCAAATTTTTCATTATTTTTTTTAGAAAAATATTTAAGCTTATTTTTTGAATAATAAACCTATAAAACAATGTTTTTGGTAAATTACACTATTTGCTTTAAACTAAGATTTTTAAAAAAATGTTTTTGTTTTTTTAATAATAAATTATTATTATATAGGCGCATTTAGAGAAAGGGGCAAATTATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTATATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGAAGTAGTGATGTTTTTTATGTATTTAAAGAAGCAGATAAAGAATTCCTAAATAAAATTTATTATCGTTATGAAACTTTTGAACAATTCGAATTATATGAAGATCAAAAAGACATTAAAAAAGTAGCCAAAATTGAAGTTTATTTTGAAAACACTCCAAATGTTGGCGATTTAATTAAAGAACTTGAAAAACTTGATTGCCAAGTAATTGTGACACATATGAATCTTGAAATTATTTCTAAAGGAGCTTCAAAAGGAAATGCTATTCTTTGACTTCTAAAACATGTATTAAAAGGATATAACCCAGAAGAAGTTATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAGTCAGTGTACTAGTTTTTTTAATATTTTGAATATTTTTAATAAAAAATAAGCAAATTTGTCAAAAAACCTGCAATATTACATACTTTTTTTACAAAATTAATCAAAAATTAATCAAAATTGTTAAAAAAGTCTGTCATTATGACAAATTAATTGTATAGTTTCTTATATAAATATAAGGAGTTGACTATGACTATTGAAAGAGATAAATATTTAAATGGTCTTTTAGAATGAAAAGATAAAGACATTGTAAAAATAGTAACTGGTCCTCGAAGAAGTGGCAAAACTTTTTTATTATTTAATCTTTTTGCTTCTGAATTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAAATTAAAGTTAGAGAAAAAAATAAAGATAATCAATTAATCACCAAAAACTTAGAAATAGATTTTGCGGCTTCGAAATATGGTGAAGAACTTTATGTTCAAGTAGTTGATAATTTAGAAAATTTTGACATTTTAAAAAGAGAAATTAAACCTTTTTTAAAATTAAAAAATAATTATCCAAAATTTATTTTATATCGCACTAAAAATATTGACATTGACAATGTAGATGGTATCAAATAAAAAGTATTTGTGATTTCTTATTAGAAAAATAATTATTTATATATAATTAAAAAACTTAATTTAATATAGGAGGAACTATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGAAAAGTTATTGAAGCAAATAAAACTACTCCAGTAGCTGAAGTTGAAGAAGTTGAAATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGAGATGATTTAATTAAAAAAGGAATTGAACCAGGTGATACTGTTCGAATTTTAGATTATGAATTCGAATGAGATGGCGAATTTTAGATAAATTTTTAATTAAGTTTAATTGATTGATAGTATTAAAAATTAAAAACATTTTAAATGTAATATAATTTTATAGAATTATTTTAAGGAGTCCTATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGATTAAATAACGATTTATTAAACAACTTTAAAATCATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAAATTTGATTTAAAAATGACAGCATTACACTGTCATTATTTTTTTAAATATTTTTTATCAAAAATTCCAATATAAGGGAGGTTTCTCATTTTTTCATCATAATCCAAACCAAAACCAACTAAGAATTCATTTGGCACGATAAAGGCTGATTTATCAGCTTTCAATTCAACTTTACGGTTGTGTGGTTTGTCTAAAAGAGTTATTATTTTAAATGAACGAGGTTTACGAGATAAAAGAATTTGCTTGATTTTATCAAGTGTAATTCCTGAATCAATAATGTCTTCAACAATTAAAACATCTTTATTTCTAATATCTTGAGCCAAGTCCATAATGATTTTAACACTACCGCTACTATTGCCTGCTCCTGCATAACTAGAAGTAGTCATAAAGTCTAAAACATGGTCAACAGTTATATCTTTTATTAGCTGAGCTAAAAATGGGATTGATCCTTTTAAAAGACCAACAATGATTAAATTGTCTGAGTCTTTGTATTGTTGATTAACTCAAGTTGCTAATTCTTTGATTTTAGCTTCAATTTCTTCTTGAGTATATAAAACCTTTTTAATTCGATAGTCAATATTTTGCTTATTCATTATACCTCTTTGATTTTGAGAAGTCATAGCTATCTATTATATTAATAGCTTCTTGTAAAACTTCTTTTTCACTTTTGTTATTACTATTAATGATAACATAAGGTATCTTAAATTTTTCAACTAATCTTATATAAAGCTTTTTATATAAAGAATGAAGTAATTTAAAGTATGTTTCATTTTGAGCATAATTATCAACTTCTGAAGCGCGACCTCGAGCTAATAAACGAGCTTTGAATGCATTAAAATCAATATCAATAAAGATCGCCAAATCCGGATTATCTTCAGGATCAACTATTTTTTCAAACATAGCATCAAAACCTGCTTGATATTTAGGATCTTTATCCATTAAAGTGACAACAGCAAAAACATAGTGTTCGATGTTGAAACGATCTAAAAAGATATGATTGTCTCTAAAATTTAAGTTATTATTTTTAAATTTAGCTACTGTTTTTTTAAATGTGTCTGATAAGCTTTCAATAATATATGATTGAAAACCAATGTGAATATTAGGTTGTTTTTCATACATTCATTTTAAAAATGTATTAAAAACTGGGTCATTTTCTTGAAATTCTTCTAATAAAACTGATTTTTTATAATGTTTATGTAAGCCTTTAGCAAGTGTACTTTTACCACTGCCAATCATGCCACTTATACCAATAATCATTGTAAGTCCTATTTATTGTAACGTTCCATTTTACTGAAATCGTAATTGTTAATAACTTCAACAGCTTTTTCAAAAACTTGTTTTTCAGTTAAATTATCTGTTTCAATAATAACATAGTTTAAATCATATTTTTTAGCTTGTTTAATAAATAATGGTTTATAAAGATCATAAAGTTCTCTAAAGTAATCTTTGTTTTTATTTCAGTTTTCAGTTTCAACTTCTCTACCTCTTTCAAATAATCTTTTCTTGAAAGTGTCAAAAGTCATATCTAGAAAAATTGCTAAATCTGGAGTTTCATCTTTAGTAATAAGATGAGAGAAAAGTGCATCATAACCTTCTAAATATTTTTTACCTTTAGGTCTTAAGTTAACATTAGCAAAAATGTAATGTTCAATACTAAAACGATCTAAAAATATGTGATTATCCTTGTATTTATAGCCTTTTTCGCTAAATTTTTTAAAACAATCTGCTAATTTAGATGTGTGGTTTTCCACTACATAAGATTGAAAACCAATTGTTAAATTAGGTTGTTTTTCATAAAGTCATTTTAAGAATGTATTGAAGACATCATCGTCCTCTTCATATTCTTTTAACATCATTGAATTTTTGTAGTGTTTAACTAAATTTCTTGTTAGTGTGCTTTTACCACTACTAATCATTCCGCTTATACCTATAACCATAATAATTACCTCTTTCTTTATTTGTTTTTAGTCTAAAAAGTTAATAAAAAAACAATACAAAACTGATTCATGTATTAATCATTTAAATGAAATCAATTGAATAGTCAACTATTTGTATTGAATTGATAAGATTTTTATACTGTATTTATTAGGCACATCAACTTCAACTGTTGCGCCTTCATCACCTTCCATGATAGCTTGTGCTACTGGTGATTCATTAGAAATATTACCTTCCATTGGATTAGAATCATGAATACCCATGATTTTAACTGTAATTTCTTTATTTGTTTTTAAGTTAAGATATTTAACTGTTGCTCCGATACCTGCTTTTAGTTTACCTTTTTTGTTTTGGTTTGTTTCAATAACTTCAGCATTTGCTAAAATGCCTTCAAGTTCAACAATTCTTGCTTCAACAATACCTTGACGATCTCTGGCTGCATCATATTCAGCGTTTTCTGAAAGGTCACCTTGTGCTCTAGCTTCTTTTAAAGCTGCTTGAATTTCAGGTCTTTCAACTTTAATTAAGTGTTCATATTCTGCTTGATATTTTTCTAATGTTTCTTTAGCTAATATGATTTTTTCTTGCTTTTTACCTGCCATCTATCCCTCCTTATATGTTTAATAATAGTTATTTTACAATATATTAAGCTTTATTTTATAAAATAAATTTATTTTTTGTCCATTATTTGAGAAAAGCTAAAAAACGATAAATAATCGATAAAAAATCAACTTTTTAGTGATCTAAATTTAATTAAATATTTAAGTGGAATTTTTTCTTCTTTATATAGAAAAAAGATTTTTAATATAGAATATTAATTATGCATAAGAAAGGAATGAATATGAAAGTTGAATACTTAAGTGAAGCAAAAGTCGAAGAATATAAATGACTTAAAAATTTTTCGAGTAAAGTTCTTATTTTTTGAACAAAATAAGATATTTCAGGAATTAAGGGTTTTAACTCCCATTCGAAAAACTTCAAATTACATCATCCAATTTCAAAATGCCTGAAGTATGTTCGCGAATTTTTTTGATTATTGTTGTTGCTTTCATAATGAATAAATTAATTATTTAGTTTAGTTTTTGTTAAAAAAAGAACATTTTCGTTACAAAAAAGTCCTTAGTTTTCATTTTTTATTTCAGTTTTACTTTTTACTATATATAAGAATCCGCTTTTTACATAACTTAATTCATAAACAATTTTATTAAGTTTTAAATGTTTAGTTAAAACTCTATAACCACTTTTTGTATTTGAATTTTGTAATACAACAAGCATCCCTTTTTCACTTAACATTGGATAATATTTGTCAAATAATTCTTGATTTGTTTCAGTTGAATTAATTGAAAAAATCAATTTGAATTTCTTATCTTGAGTTTTTAATTCATTAACATTAATAAATGAAGGTTGATCTTTAAAGTATTTAGGTAATTCTAAAACAGCTTTATTTCATAAACTTACATTCATTTTTTGTTTTTCTAAACAAATTTGAGCTTGTGATTTATTTTTAAATAAAGCTGTTGGATATTCTAACTTACTTCCAACAAGTAAAATCTCTTGAACATCATTTAAATAAATAGTATTAATAAAGTTTTCTAAATCATAATCATCAAGATTACTTTTAAAAAATTCTTTTAATTCTTTAGGGAATTCTCCTAATTCTTCACCTCTGATTTTCAAAATAGCTTCATGAGCTTTTTGAGCTTCTTTGGTATTTATTTCATTACGAATTTTTCTAGTTTTAACTAGTAAAATAATATAAGTTATAAAGGCAATAATCACAAAAACAATAGCAGAAACAATTAAGCCAATTTTTGCACCTTTACTTAAATTGATTACAGCACTAATCATGTAATTCTACACCTCCATAATTTAAAAATTCTTGCAAGATTAAAACAGCTGACAAAGTATCTTTATGAGCTTTTCTTTTTTTAATAGAAAGTTTTGCTTCTTTTAAAATATTAGTAGCAGCTATAGTAGTTCCATATTCATTAACTTGATAAATTTTGTAGTCTTTAAAAATACCTTTTAGTTTATGAGCAAAAGATTCAAAAAGCACTGTTCTTTCACTACTTGTATTATTAGATCTTAAAGGCTTTCCTAATACCAATTTATCTATTTTATATGTTTTTTGGTAAATTTTGATTTGCTCAATAATTGCATTAAAATCATTTTCTTCAAAATGTAATGTTTCAAGTGCACTAGCTATAATCATGCCTTCATCACTAATTGCAAAACCGCAAGTTTTAGTGCCTAAATCTAGAGCTAAAGCTCGCATTAAATAAGTTCCTTAATAATATTTTCAAGATCTTTAGTATAGTCAACTTTACCCATTGAAATAATTGAACTTCCACCACCACGACCATTGGTTTTTGAAGCTATTTGTTGGAATAATTTGTTAGCATCAAGCACTTTAGCGGCCACACATAAAAGAATTTGATTATTACTTTTAGCACCTAAAACAATTATAGCTTCAGGAAATTTTTCTCTAAGCGTTGCAGCAGCAACTTTAACTTGGGAACTATCAAAATCAATGTTTAAATAATAAGGGTATTTTTTGTTTTTAGTTAATTGAACACTATCAAAATCAAAGGTTATATTGCTTTTGTTTTTGATTAATTCTTTGATATCTTCATGAACTTTATCAATTGTTTTTTTAATGTTTGCACATTCAATTTCAACATCTTTATTAGGTGTAAATTTAATTTGATATTTACTATCAAAGCTTTGAGCTTTTTTAACTAAAGCATCTAATTGTTCTTTTAAACTTTGATTTAATTCTGTTAAATATTGTTTAACATATTTATGAGAAGCTACAGCTCTTATTCTAAATACACCAGCAGTTTTTTTCTCAACTTTAGTAATTTTAAAGTCTTCTAAAATTCCGGTTCTATCTAAGTGAGTACCACCGCAAAGGTCTTTTGTAATACCTTTAAAGTCAACAATACGAATGTTATTAGGATCCATGTATTCACTTTCGTCAATAGTCATAATTGCGCCCATTTCTTTGGCTTTTTTAATGTTTGTTACAATATATTCACGTTCAACATTTTGTTTAATTCAACCATGCATTATGTCTTCGATTTTTTTAATTTGTTCATCAGTTGGTTTTTCATCAGCTGGAAAGTCAAATATTAAACGTTCTTCGGTGATATCTGAACCAAGTTGATCAATATGAGGTCCAAGTACTTTAACTAAAGCACGGAATAATAAGTGAGTTGCTGAGTGACCTCGAGCCATACCAATTCTTACTTCTTTATCAACATAACATTCGACAGTATCCTTATTGTTAATTTTGCCTTCAATTTTATGAATATGGTTACCAAATTTGTCTTTAAAAACATCTAAAATTTTGATTTTATTTTTACCTTGTAAAATGAAACCATGGTCATGATTTTGACCACCACTAGTAGCATAAAAAGGTGTTTTATCTAAGATCAAATAACCTTCACCATTTAATGATTTTACCTCTTCCTCATTATTTAAAAGTTTAAGAATTTTTGATTTACTTTCTAAGTCTGTATAACCAATAAATTCACCGATTTTGCCTTTAATTAAGGCTAATGAGTTAATTACTTTATCCATTCCGTTAACTTTTTCACCCCTGCTTAAGTTGGCGTGTCTTTCCTTAGCTTCTTCAAAAGCTTTAAGATCAACTTTAATATTTTTCTTAGCTAAAATTTCAACAGTTAACTCAATTGGAAAGCCATAAGTTTCAAGTAAATTAAAAGCATCTTCACCTGAGAAAATTTTAGTTTTATTATCCATAAATTTTTCAAGTAATAATTTACCTTTTTCAATAGTTTGATGGAAAGCTAATTCTTCTTCTTTAATGGCTACTACAACAGGTTTTTTGTCATATTCATAAGGTAATGAATCTTGTACTACATCTACTAATTTATGTAAAAATAGACCTTTAACTCCTAGTTGCATTCCTTTGTAAATTGAACGTCGAATAAGTCTTCTAATAATGTAACCACGGCCAACATTTGAAACTTTAGCTCCATCAGCTATAGCATTAACCACTGTTCTAATGTGGTCTGCTATAACTTTAAAGTTAGTGTTGATTTCAGTTTGCTTTTTATCTTTTTTAAAGTAGTTATCAATGTCATATTTAACTGTGGCATATTTTTCTATTTCTTTAATTATGTTAATAAAAAGATCGCTGTCATAGTTAGTTGGAGCATCTTGCATAATTGAAGCAATTCTTTCAAGTCCTGCACCAGTATCAATATTTTTTTGTTTTAATTCAGTATAGTTATTTTCACCATCATTATTGAATTGACTAAAAACTATATTTCAAATTTCGATGTAACGGTCATTTTCAATATCTTTTTTAAGCAACTCTAAACCACGTTTGTTATATTTAGGTCCTCTATCATAAAAGATTTCTGTATCGGGTCCACAAGGTCCACTACCAACATCTCAGAAGTTTGTATCTCTAGTTCCTGGAATTAAGTGTGATTCTTCAACTCCTTGTTCCATTCAGTATTTTTTAGTATCCAAATCTTCTGCAAAATAAGTCATGTAAAGTTTTTTAGGGTCTATTTGTAATTCATTAGTTAAGAATTCATAACCAAAAGCGATAGCTTCTTTTTTAAAGTAATCGCCAATTGAAAAGTTACCTAACATTTCGAAAAATGTGTGGTGTCTTGAAGTTACACCAACGTTTTCTATATCATTAGTTCTAATTGCTTTTTGACTGTTAGTTAATCTATTTCTAGGCGGTTTTTTCTTACCTGAAAAATAATCTTTAAGTGTTGCTACACCTGAGTTAATTCAAAGCAAAGAAGGATCATTAACTGGAATTAATGATTTGCTTTCAACACGCAAGTGACCTTTTGATTCAAAATACCTTAGTCACTTTTCTCTAATTTCTTTTGATGTTAATTTTTCCATAGTTATCTCCTAAATGTAATCTTTTTTAATATTGTTTAAGTATATTTCTTCAATTATTGCTCCGCCTAAGCACTTGTCTTGATCATATAAAACAACTTGTTGACCGGGGGTTACTGCTTGAGCTTTTGATGGATAAAATACTTTAATTTTATTATTTTCTAAGAATTCAACTTTAACTTTAAGATCTTTTTGACGATATCTAAATTTAGCACTTAATTTGTTTTTATTAAAGCGATGATGATTTAGACATAAGTTTGAAGCTATTAGCATATTACTTTCTAAATAACTAGGATCACTAGCTGGTGCAGCATAAATAATATTGTTTTTGACATCATGACCGCAAACATAGTGAGCTTCACTTTTGCCGCCTAAATTTAATCCTTTTCTTTGACCGATAGTGTAATAGAAACAACCATCATGAGTTCCTAAAACTTCTTTAGTTTTAATGTCTAATATTTTTCCTTTTTGAGCTGGAATATAGTTTTGTAAGAATTTTGTAAAATGTCTTTCGCCAATAAAACAAATACCTGTTGAATCTTTTTTTGTAGCTGTAGCTAAGTCTAATTTTTTAGCTATTTTTCTTATTTCTTCTTTAGGATAATCAGCAAGAGGCATTAAAACTTTTTCTAGTTGTTGATTAGTTAATTGAGCTAAAAAATATGTTTGATCTTTATTTTGATCTTTCGCCCGATAAAGTTTACCATTCTTAACTTTGGCATAATGACCCATGGCAATATAATCAGCATTGAGTTTTTCAAAAGCATATTTTAAGAAAGCATCAAATTTAATATATTTATTGCATAAAATATCAGGATTAGGTGTTCGACCTTTTTTATATTCTTCAATAAAGTTTTCAAAAACATTATCTCAGTATTCTTTGACAAAATCAACACGATGAAGCTTAATACCTAATTTATTTGCTACTGCTAAAGCATCTTGATAATCTTGTTCTTGAGGGCAAATATCTTGACTAATATTTTCATTGCCTAAAAAATCATTATTAACATATGAATCTCAATTCCGCATAAAAAGACCTTCAACATCATAACCTTGTTGAAGTAATAAATATGCAGCTACTGAAGAGTCTACTCCTCCAGACATTCCTAAAATAACTTTCTTTTTATGCATATTAATCCTTATATTAAATATTAGTTTTAATTATATTATTAATTTTTATATTAATAATCATTTTTTAAGAAATGTCAAATAAAACCACACAAGTGTGTGTCTTATAATTATCCTTCAATTCACCTAGGTGGGTAATTTGTTTAGTTTTTAGCATTTTATTTTATTTCTATAATTTTATTGTGAATTTCATTTTCGATAAAGCTTTTCCGCTATTTTCTATAGTAAATACTTGAGCAAACTCATCATAGCTATCTTTAACATTTTATTTTAAAATATCTTTTAATGAAGCCATTTTTACTCTGTTTCTATTAACATCTTCATCAGTTAATTTTTGTATTAATTTTTTTAAAATAAATCTTTTAAATGTTATTAGAATTTTATATCTAATAAAATTACTAAATAGACTGTTTTATAGTTAAATTGGACAATTTTGAGTATATTTTTCAAATCAATATTAATCAAAAAATAAAACAATCAAAATTAAACATTCAAACAGTTGATATATAAGTTTAAGTTAATTAATATTGCCTATTTTTAATTTAGTTTTTTATTGTCCCTTTCTATTTTTAATCAAAATATAATTATTATTTTTTCTGAATCTATTAAAAAAACATAATTTTTTGACTCTTTAATAGCGAATTAGAATTATAAAAAAGAAGCACTATTGAGTACTTCCTAAAATTATTTTGAATTACATTTTGTGTTTTGAACTAACACCTAAAAGTCTTAATCCGTTTTCTAAAACAATATTAACAGCACGAGCAATAGTTAAAGAATTCATTTCATTCTTGCTTCCAATAATTTTTTCTGTATTTGAGTATCATGAGTTAAATTCTTTGGCAACTCTAATTAAGTATTGTGTTAGTAAGTGTGTTAAGTACTTAGTTGCTGAACTAATGATAACTTCTGGAAATTCAAGCAATGTCGAAACTAATTTTTTATCAAGTTCGTTGTCAATATTTGTTGCTTTAAGTTTTGAAACATCTAATTTAGATTTCTTAAGCAATTGGTATGAACGAGCATTTGCATATTGAATTAAGAAGACAGGGTTGTCATTTGATTTTGAAGTTGCAATGTTAATGTCGAAGTCAAGTTTTGAATTATATGTTCTATCTAAAAGAATGAAACGAGCACTGTCTACTCCAACAAGGTCAACAAAGTCTCTTAATCAAAAACTTGTACCTTTACGTTTTGACATTTTGTATTCTTGACCATCTTTCATAAGTCTTACTAATTGTAAACAAAGAACAATTAATTTGTTTGGGTCGTAACCTAAGTCTTGCATTGCTATTTTCATTCTAGTGATGTAACTTAAGTGGTCAGCGCCTCAAATGTTCATAATAATTGGATCTTTGCCTGAGTTTTTGAATTTTTTGTCATGGTAAGCAATATCTGGTGCAAAGTAAGTAAAATCACCATTGCTTTTAATTACAACACGGTCTTTATCATCACCATATTTTGTTGTTCTAAGTCATGTAGCACCATCTGCTTCAAAATGGTTTTTAAGTTTTTTAATTGTGTCTAAAATTCTTTTACTGTTGTTTGCATATAAAGTTTTTTCACTAAATCAAATATCAAATCCACATTTAAATTTTTTAAGATCAACTTTAATTTGTTTTAAGAAATTAGCCACACCTTCTTTTTTAAATACTTTTAAAGCTTCATCATTTAATTCTTTAAGATCTTTAAATTTATCTCCATGTTTTGTTCTAATTTGTTCAGCATTTCAAACAATGTCTTGACCTCCATAGCAGTCTTCTGGCATAGGGAAATCTGGTTCATAAAATTGTTTATATCTTGCAAATATACTTTGAGCTAAAGTGTTAATTTGGTTACCACCATCATTGATGTAATATTCACGTAAAACATTTAAACCTGCGAAAGTACAAATGTTGTATAGTGCTGATCCAATAGCAGCATTACGTGCATGACCAATGTGCAAGTATCCAGTAGGGTTTGCTGAAACCCATTCGATGTTTAGATTTCCTTTTAATTTACCACGACCAAAGTTTTCATTTTGTTTGATAATTTGGTTCAAACTTTTAATAATTGTTTGATCATCAATGTAAAAGTTTAAAAATCCAGGAGCTGCAATTTCTACACTTTTTAGACCTAATGAAGTTAGTTTAGGCTCTAATTCTTTAACAATTTTTTTGGCAAGTTCCATAGGATTTTTGCCTTTGTTTCCTAATGCAACGTTAGAAGCGAAATCGGCTTTGACTTTAGGTTCAATTAAGAGATCGTCCTTTTCAATCCCTAATTTGTTTAAAATTGGTTTTAATTCTTTAATAATTTTGCTTTTAATAATGTTTTTATCCATAATGTCAATATTATAACAAATTAATAGTTAAAATATATGCCTATTTTAATATTTTATTTTTTTGTAAAAATAAGTATTTTTTAAGGTTGTTTTTGCTAATTAAGCTTTTTTATAGGTTAATTGAAAAAACAAAAAACTTCACACAATAAATAATGGAGATTTTTATATGTGCAAAGTTTTTCTTTTAATTTAATTGAATGAAAAGTTTTTTTGATGTTACTTATTAAAAGTAATCTTTCAATATTGCATCTTTATATGATTCAACTTCATTTCATAAGTCATTTAAAATAGTATTTATTTCTATTATTTTTTTGTCTTTAATTAATTCGCTGTAGTAATTTTCAGATTTCATTTTTTCGCCAAGTTTTTTAGCTTTATCTCTTTTAGCAATAATTGCAGAATCAGTTATTTTTGTAGTTTTTTGATACTCTTCATAAGAGTCATTTATTTTGGCTAAATATGCTTTAATGTTTTCAAATTTAACTTTAGCAGCTTCTAAGTATTTTTCTTCATCAGTTTTACCATCTCCACAAGAGACACAAACAACTGATACAGGTAAAAATGAAGCAACTGCTAAACTTGATATTAATAGTTTTTTCTTAAACATACTTTCTCCTTATAAATTAAGCTTGAACTTCAACTGGTTTTTGTTTAGTTAAAGTAGCTTCTTCTTTTGACAATTCTGCTAATTTTTGTGCTTTGTATTCTTCATAAGTTTCTAAAGAACCATATTTTTTCTTGTTAATTGCATCTTCAATAACTGCAGGAAGGAATGTTAACATTGCAAATATTCCTAATACACATACTAATGCAACTCTAGAAATAATTAAGTTTTTGTACATTTCAGCTCTTGAAGCATCAATAGCTTTTACATATTCTTCAAGTGTTGCATTTCCTAGACTACTGTCTACTGAATAGTCAACATAATCTTTGTTAACGCCTGCAAGTAAGAACATATCAGTAATAGGAGCTAAAATAGTTACAGCTAAACTAAATCCAACTAAGATAACTGAAATTCATGCAAATGGTAAGAAGTATTTTTTCTTATTTTTAATTTCAATTTTGTTTGTTTTTCTATTTTTAATTCCGCCATAAATTGCAACAGCAATAAATCCGAATGTAAATAAAGCTGTTCAGTTAGCCATCAAGTCGCAGAAGGCATATAGTCTTGCCATTGATTGATGCGCATCACTACTATAAATTGTATAATCTGAGTTATCAGCAATATATGCTAAAGCACCAATTGTAGTAAAGATAATAACAACAGGAATTGTGATTACTAGTGAGTATATAATACCAACATATGGTTTGTTAGGATTTAATTTTTTGTGTCATTTTTCTCAGAATGGTAAGTCGCCTGTAGCTAATAAATCTTCTACATAACGAGGAGCTCACATTGAGAAACCATTAATAATACCTAGAACACCAATTGAAATACAAATGTTAATTAAACCAAAAACAATTCGACCAGCTTTTTCATTTTTGAATAAACTACTCATGTATCCATTGAAGCCAAAGAATGATCCACCGTTAAGTGACATTGAAATAGCAACAATTAAGTAAATTAAGGTTGTGATTCCTAAACCAATGAAAATTGCTAAAGGAGTCTTTTTAGGTTCTTTCATTTCACTTTGAATTCCAGCAGCGACATAGAATCCATCATAAGCAAAGAAGATAGCAGCAACAGCAAGGAATACACCAAGACCAGCTCCTAATTTAGGTAAAGCAGTAACACTTTTACCTGATTTAATTGCTTCTGCAAATGTTTGATCTGGAGCAGGCATTGTTCAGTTAACTTCGTTTAAACCACCATTTCCAGTGAATGCTAATACAAATCCCATAATAGCAATAAATACTAATGGTATAAATTTAACACTTAAAACAACAATGTTTTGAACACTACCAACTTTTGATCAAATTGCAGGTACTGTTAAGAAGTAAACAGTCATTAATAAGCTAATTGATAATCAAATAAGTCAGTCAGCATTTGTTCCGAATGTTAAAGCATCTTTATTCATTAATGCCCCAACACCATCTTGAAGTGACATGATTACATACATAGGCATAAATAAGTATGTAAGAGGTAAATAGATATAGGTCATAAAGTCTTTTGAAGCGCGGAAAATCCGTTTGCTGTTAAAGACTTTACATCAGCTCATAAGTGACAAGTTGTCATTTTTAACTGATGAAATTTCAATCAATGATAAAGCCATTGCAATAACCGCAAATGATGCAATTATTCAACAGAAAATGGCTAGAATCAAACTACTGTGCGAGCTTTTTAAAACAGCACCTGATTTAAAGAAGATACCAGCACCAATTGATCCACCTATAACGATCAACATAGCAGCTATAAAGGAAATCTTCTTTTCTTTTGTTTGTTTTTCTTCCATTATTAAATTTCCTTTTTAGTTTTTTAATAAAGTTTTATTTTTTATTTATTGAATTATCCTTTGATAATTCTTGTACCACTTTTGCCTTCGATAGCTTGTGATACTTTGTTTAAGTCAGCAATAACAGCTACATTTTTTGGATTTTCGTTAACAAAAGCAATAGCAGCTTTAACTTTAGGAAGCATACTTCCTGGAGCAAATTGTTCTTCTTTGATGTATTGTTCAAGTTCAGAAACAGTTACTTCTTTTAAAGCTTTTTGGTTTGGTTTACCAAAGTTAACACAAACATGTTCTACTGCTGTAAGAATCACAAATAAGTTTGCTTCAACTTTTGTAGCCAATTTACTTAAAGCGAAATCTTTGTCGATAACGCCATCAACACCTTTATATTGTCCTTTTTCGTCGATAGTAGGTATGCCACCACCACCACCAACTATAACAACATTATCAGCGTCAAATGTTTTTCTAATTGTTTTCATTCCAATAAAGTCAAGAGGAAGTGGTGAAGGAACAACTTTTCTAAAACCACGACCAGCATCTTCTTTAATGGTTGAATTAGGATTAGCCAATTCAGCTTCTTCTAAAGTTTTATAGAAAGGACCAACTGGCTTTGTAGGGTTTTTAAATGCTTTATCTTTAGCATCAACTATTGTTTGAGTCAAAAAGTATAAAACATCTTTTTCAATTTTTTTCTTTCTCAATTCATTGGTTATCGCTGTGAGCATGTGATAACCAATGTAGCCTTGAGACATACCACCTGCTTCTGCAAAAGGTACTAATGGAGTTTTTTCATTAACTTTTTTTGCATCAGCAAAGGCATTGAAGATCATTCCAACTTGAGGACCATTACCATGACCAACAAGCACTTGGTGACCTTCTTTAACTAAATCTGCTATTTTTTTAGCAGGTAATTTAACTAATTCTTTTTGTTCTTCAGGATTGTTTCCTAAAGCGTTTCCACCTAAAGCTATAACAATTTTTGACATAATTTAAATCTTTTAAATTTTAGTAATTGTACTGTTTTATAAGACTATTGGGAGAATTAGTAACCAATAGTTGCTAAAACAACAGCTTTAATTGTGTGTAAACGGTTTTCAGCTTGATCAATTGATCTGTTGCATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCTCCAGTTGCAACTACTGGGTATTTTTTACCAAATTTTTCTTTGATTTCAGCTGAGAATAATGTGTGGTCATCGTGGAAAGCTGGTAAGCAGTGTAAGAAGATAACGTCTTCTTTTGCAGCTTTAATCATTTTCATATCAACTTGGAATGCTCCTAATTCTTTGATACGAGGTTCAAATAATGAGAAGTCTTCTCCTAATGATACTCAAACATCTGTATAGATTGCATCTGCATCTTTAGCAGCTTTGATTTTGTCGTCTTCAAATGTAATTGATCCACCATTACGTTTGAATAATTCTAGACATTTTTCGTAAACTTCTTTGTAACCTTTTCCAGTTTTTACAATTTTTGCTTGTTCTTTAGGTCCGCATAGAACACAGTTCATTCCTGTAAAAGCAGCACCAATCATTATTGAACGAGCAACATTATTTTTGATGTCTCCAGCAAAGACAATTTTTTTGCCTTTAAGATCACCTTTGAATTCCTTAATTGTCATATAGTCTGCCAACATTTGTGTTGGGTGTTCGGCGTCTGTAAGTCCATTTCAAACTGGAACTCCAGAATATTTAACTAACGCATCAACATCAGATTGTTTGAATCCTCTGAATTCGATACCATCATAAAATCTTCCTAAAACTTGTGCAGTGTCTTCGATAGATTCTTTTTTACCAAAGTTAGAACCTGCAGGTCCGATATATGTGCAAGATGCTCCTAAGTCAGCTGCTGCCACTTCAAAGGCGCAACGTGTTCTAGTAGAGTCTTTTTGGAACATGATAGCAATGTTTTTTCCAACAAGTGGACGATTGTTAGCATGTAACCCTTGTCTTTTTGATCTTTTTAAATCAATAGCAAGGTCAATAAGTGCATTAATTTGATCTGTAGTGAAATTTAGAGCTGAGTCTAAACTCCTACCTTTTAAGTTTAATGGCATATTTTCTCCTTAAATTAATTAATAAATTTATTTTTTTGCTAAATTGGCACTACGGCGCAAATTGAGCTGATAAAATTTTATCACTATGATTTATTTTTTCTTAATGTCTTCTCTAATAAGAGGCATTGACATACATCTTGCTGAACCCATGCCTAATGAAAGTTGGTTTCCTTCGAATGATAAAACTTTAACTTTAGCTGCTTTAAGAGCTTTTTCAGTTTTTTCATTTCTTGAATATCCAACTACAACACTAGGTGCAATTGTCAAGTAGTTTGTACCATCGAAGTGAGTTTCGATATCAATATCTAATTGACTTGCTCCTTTACCTGCGATAGGAATTAAGATAGGTTTCATACCAATAATTTGTTCAAGAATTTCTTCTAATGAACCTTCAATTTCGTGTCATACAAAATCAGGGTCATTTAAGTCGATTCTTCAAACTTTTAATACTGATAACATGTTTGGTGAGTATAAGAATTTATTCTTATCTAACATTGTTAATCATGTATCTAAGTGCATTAAGTTTGGCATAGGAGGAACATTGATTGCATAAATTCTTTTGAATTTAACTTCTTTGTCCGCTGCTATTTTACGAGCCATAACGTTAATAGCTTCTTTGTTAGTTCTTTCGCTAACGCCTACTACTAATGTATCTGCTGAGTAGATAAATACGTCTCCACCTTCAATTCTTCCTTCATCTTTTCTGTCGAATCAACGAGGTACTGTATTGTAATCAAGGTTATTGTCAAAGATAAATTCTGAGAATATTGTTTCTCTTTGTCTTGTGACATATTTCATGTGGTGTAATGAAATACCATTACCAACTGATGCAAATGGATCACGTGTAAAGTATAAGTTTGGCATAGGGTCAACTACAAGATCTCTATCTAATTCGATACCAAGTTCTTTTTTGTCGATACCAGCCATCATAACTCTAACCATTTTTTTAGATGATTTAGCTTTAAGTTCAAGAATGTATTCTTTTACTTTAGCTCTTAATGTAGCATCTAATTTTGGTTCGCATTCATCTAATCATCTTTCAATTAATGATTGCTTAACTGATTCGCTAACCAAGTCAAAAGTTTTAGCGAATAATTCGTCCAATTGAATAACTTTAATTCCGTTATCTTTTAATAATTGAACAAATCTTTTATGTTCCGCAATTGCAGAATCTGGTTCCAAAATTGCTGAAAATAATAGTTCATCGAGGCGAGAAGGCGCGATGCGCCTAATTTCGTCTCCAGGTGTGTGAACAAGTACTTCCTTAAGTTTTCCGTATTCACTGTAAACATTAATTTTTTTCATAATTAATCTCCCGATAAAATAAATTTTCATGAATATTAACATATTTGTTAATAAATCAATTTTTGCAATGATTTGTAAGCGTAGCTTATATTTCTTTTAGATATAATACGTTAGTCATTGCATATAAATTATAATTTTTTAATAGGTTAAAAAGGCAAAATTGTGTTAGTTATGGAAATTTTCTCACATTTTGATACATATAATAATATTAATTCTATTTTTAAGTATTAAATAAGCAATAAAAAAGCAAAAAACATAGATATTTATTCGATATCTATGTCTTCTTATTTGTATATTAGTTGTTTTTGATTACTAATTCTAAGCTATTAGGATCAAAATCATAATATTGGTTTTCCTTTAATTTATACTCGTTTTCGTAAATTTTAGGAAATACACTAGCTACTATTTCCATAATAAAATCATATGTCATAATCATAGCCATAACATCATTTTCACAGTATTCTTTTAAATATTCAATTTTAGCTTCTCAAGCTTTGTCACCAATGATGCCTAATTTACGACTAATTGATTCTTCTAAAGCCATTGATCCATTTTGGATTTCAAGAGTTTTATAAGGTTTAATAATATGTCTTAATTTAAATTTATTTTCAGTGATAATGTGTTCAATTTTTTTAATACTGGTTTTGCCTCATAATTCATAAATGAAAGTTGAAAATCTTTCGTCTTTATAGTAAGTTGCTAAATTTTCAACGTTAAAAGGAATACTATTTTTAGAAACTGAAGTATATAAAATTTCTTTATTTAAATTTTCACTATGAAATTTAGCTTGAGGGACATAATTAGTTTTAAAAACATAATAATTTTTATTAGCATCCATGCCATAACGTTCATCGAATTTATTCTTAGATATTGGTTTAAAACAATCTAATAAGTCAATAGTATGTTCATTAACATAAGTAATTAATTGTTCTAATTTTTCCATATCGAGCTTATTTATAGATAAATAGTGTTCCATGTCTTTTTTATGTTGACTATCAGCATAATAAAGTTTAGCTAATTCTATAAGTTCATTGTTTCTGGTATTTTCATAACCTTTGTTATAAACAATGTAGTAGTCAGCTTTATTTGAATAAACAGCAATAATATTGTCTAGAAGATCATAAATCGTGATATTTTTAGGGTCTTTAACAATATTATTTTTATTTGTTATTTGTCCATTAATTGTTTCAATAATTGAAACTTGGTGAGTGATTTGAATATAAGGATTCAAGTAGTCAATTATTGGAAATACTGAGCTAAGACCTTCATAGTCATATCAAATAATTCTTTTGTCTTTTTGGTGTAATAAAGATAATATTTCTAAAACAAAAGATGAAAAGTTATTATGGAATGATCTTTTTTCCTTAACTTTTTCTCAAAATTGATTTAAGTATTCTTGATCAAAAGTTTTTAAACATTTTTTGACAGCAGGAGAATATTGTAATTCAAGAGGTGTAACAGCTACTCTAACTTTATTCATTAAATCTTTGTTTTTACCATAAAACATATCTGAATCCGGCAACATAATTTGATTATCAAATGAAAATGTTGGTTTAGTTAAATAATAAGCTTCTACAATTTTACTTATTACTTCTTTAAAGTCTCCAAATTTACTTCTTGGATCTTTGCTCATAAATTCTAAAGAAGTTTTTGGCATTTTGCGTGCACTTGGTTGAGTGAAAATATCTTCTTGAAGTGCGGCAAAAAGGTAATCTCCATGAACATTAGCTTCATTTTTTGAAGCAAAATAAGAACCATCATTAATTGTGTAACCAAGATCACTTCTTAAAGCCTTTTTGTCCATTTTGTTTTTACCACAACTTGAAGCTTCAATTAATCTTAAGTTTAGTTTATCTTTTCTAAAAACAAAACCATTTTGAGTATAGTCATCAAAAATAATATTTTGAATTTTTTTAACTTCAACTTTGGCTTTTTTAGCTACTTGATATAAGTAATTAGCTTCTAAATAATCTTTATTAGTTGTTTTAGAATTCATTCTTTTAAAATAAATAATTTTATTAACTTTATCATAAGCAAAAGGTTGGGCTTTTATTGTAAATTCTTCTTTATTTTTGCTTAAAGCTTTAAAAGAAAAAACAGGATTAATAATGTATTTAATTTTGCTGTCTTTTAAAGCTTCCTGTGTGTTAATTGTAGCTTCTTTAACGTCTGCTACTTTTTTGAATATGGCTGTTTCATAGTTGTTTTGGACTTTTCAAATGTTATTTTTTAAGAAGTCGATATATTGAGATTCAATCTTATCATAAGCTATTAAATTAGCTCCTTTAAAGTCTCAAGGAGTGTTTTCTGCTTGATAAACAAAATCACTTCAAAATGTTTCTTCTTCACCTTCTTCACTTTCATCATCGCTAAATTCAGCAAGGTTTAAAGAATGATAATTTTTAATTAAAGGCAATGAAAAGTTGTTAGCATCTTTATTTTCTAAATCACTATCTAAAAGATTGTGTCAAATGTAGTATTTTTGCTTAACAAAAAATTTGTAATATTGGCCAAAATTAATTATTTTTTTAATTGAATTCATAATCTTCATCAACTTTTCCTAATGATTTTCAACGATCAAACAATTCTTTTCATTCAGCTTCATTATGGCATCTTAAATAATCAACTAAATCACGATCTTTAGTTAAATTGTATTTAGGAGATTCACCCAATTGTGAATATCTTTTTCAAAATTCAATTATGGTATTTGCATCATCTTCAGCCATTTTTTTATCAACTTTAAAAGGATAAGTTGCAAAATATCTTTCATTAATTGGAAAGTTTTCAGGATCGGCGTAGTCTTCTTCTTTTAAGAATGCAGCTGCAATCATATATTTGTCATAGCCTAATAAATAAGCATAAAGTTGGGCTTGTTTTTTGTAATCAAGAGGTACTTTATTATTGGGAGCATCTCAATCAACCTTTTTCTTTTCTTGAACAGTTTTAACTTCAACTACAATATTTTTTGAAGGAATAAGACCATCAGGAACACCACCTAAAATGTCGTGTTTGCCTTTAAAATAGTCATATCCGACATTTGCTGCTTCAATATGAATTACTTTTGTTGGTTGACTATCAGCACTAACTTTACCTCTTTTAATATCTTTTTGATGCATTTCATTAAATTTATTTTGCATAAGTTCAAAAACTTTAGGTTCTATAGCTACCCCGGCATCTATATATTTAGTTTGTAACACTGGCATACTTAATCTTGACATAAAACAAAAAGCTTGGAAATGACTTTTGAAGCTATCTGTTTCTAAGACGTTACCAATTGAGCTACCACCAAATTTTTTGAAACCATTAAAAGTTCCTAAACTATTTAAAAGTGTGTTATGAAAATTGGTTGTTAATTTAATTGTTTGTTTTCTTTCATCTAAAATGTAATCTTGTCCATTATAGAATTTTCTTTTTGTTAATTTTTTAACTCTGTTCATTTTTATCTCCTTTTAGTTTGTTGAATTTTCTTCTTTATATATTTTGTCTAATCAATATTTTATAGCAAAAATTAAGTCTCTAAAAGTTTCTATTCTTTTTTCAACTTTAGAATCATTGTCATCTATTGATTTTTTATAAAATAAATTATTGCAAAGTTCTCGTTGTAAAAATCGATGAACAACATTATTTCAAAGAATTGTTAAGATTCTTGAATCTTCAAATGAATCATGCATATTCAATTTTTCAAATTTATATTCTTCTTGAAAAAGTATATTTATTTTTTTATCTTTATTTTTTAATTCTTCTTTTAATTCTTCACTAAGTAAAGCATCATCATAAGTACAAAAATATTTTGCCACATTAAATAATGTTTGTCTATACATTGTTTCTTTTTCTTTACTAATTAATTTTTTAATTATCATTGTAAAATTGCAAACATCAATATAATTGACTAATCGACTTTCAACATATTTTTTACCTACAATTTTTTTCAAGTTGTTTAAATCGGCTTTAATGCCATAAGAAATTAAGATTTTGTTAGCATCTAAAATATTTTGAACTTCTTCTCTTGTTATTAAATTTTGACTTTGATCTTGATAAATATTGCTATATTGATCTAAAATGAAACCATCAATTTGGAAAAACTTACGGTTGTCTTTAACTACACCTTTGTAAAAGTTTTTTCTTTCTTTTTCATTAAATTCTTTATTGTTTAAGTTTGATATAAAAGAAGAATATTGAACTGGATAGTAACTAATTGCCACTTTATCATTTAATTTTGTTAATCTAGATTCAAAATCGAAAAATAAGAAGTTTTTATTGTTGAATATTTTTTGATCTAAAAATTCATCGTCGATTCTAATGACATCTTCAACATTAATTTTTTCTAATTCTTTAACACTTGTTACATTTGTTTTATTTTCTTCACATTCAGATATTTTTTGAATAATAGCTTTATAAGGCATTTTATGTCAATTTTTATTTTTTGAAACTGTATAAACAAAACTATATTTAACTAAATCGTTTTGTCTTTTGTTTGCTTCATTTTGTTTTTCTTGTTTAGTTTTAGTTTGTACTTTGAAACCAAATATTTTTTTAATTACAGAATATGATTTTTCTTTATCAGTTTTATTTTTATCTTTTGGATAATAAAAGAATTTAAGTTCAAAGAAATCATTAGTATCAATTTCATCTCCAACTATTAAAGAAATAAATTGATTATTATCTTTATCAGCCGTTAAGCTTTTATTAAATATGTAGCCTTGTGTTTCATAACATTGTTTTTTAGAAAAAATACTTGGGTTATTTTCTAATTCTTCAATAACTTCGATAAAAGTCTTTTTAATTGTTCCGTTTATATTGCTCATATGTCTCCTTTTTTACTAATAATACTATAAAATAGTATTTTTCAATGATAATGGAATAAATAATCATATATGAGCATATGTTAAAAAATCTACCATTAAGGTAGACTAATTTAAATTATTTTAATTTTAAAGCTTTATTTAAAAGTTTAATAACTTGTTCTTCACTATCTAATTCAATAGCTTGAGCAGCCAATTCTTCCATGTCTTTTTTGCTTAATTGAGATACCAATTCTCTTGAAGCTAAAACATTACCAGCAGACATACTAAATTCATCTAAACCTAAACCTAAAAGTAAAGGCAATGCGCGTCTATCTCCGGCCATTTCACCGCACATACCAGCCCATTTACCATGTTTGTGAGCACCATCAATAACCATTTTTACTAATCTTAAAATAGAAGGGTTAAGCGGTTGATATAGGTATGAAATAGTTTCACTCATTCTATCTGCAGCCATTGAGTATTGAATTAAGTCATTAGTTCCAATCGAAACAAAGTCTGCATATTTACAGAATTGATCGCTTAATACAGCAGCAGCAGGGGTTTCCATCATAAGACCTACTTCAATATCCTTTTTAGGAGCAATTTTGTGTCCTGCTTTTTTAACTTCACTATAAGCTTCTTCATAAAGTTTTTTAGCTTGCAAGAATTCTGGAATGTTTGTTATCATTGGGAACATAATAGCAACTTTACCATAAGCACTTGCTCTAACTAAAGCTCTAAGTTGAGTTTTAAAGATTTCAATTTCTTTAAGACAAAGTCTTATTGCACGATAACCTAAGAATGGGTTCATTTCGTGAGGGAATGTAAAGTATTTAAGAGTTTTATCACCACCAATATCTAGTGTTCTAATAACAACTCTTTTACCTTTCATTCTTTCAACTACTTCTTTATAAGCAGCAAATTGTTCTTCTTCTGTTGGTCAGTGTTCATTATCCATGTATAAGAATTCTGAACGGAATAACCCAATTGCTTCAGCATCATTGTCAATAACTGCATCAACATCTTTTGGACGTCCAATATTTGCAGCTAATTCAACATGATGTTTATCTTTAGTAATTGATTTTTTACCTTTGTATTTAGCAATTGTTTCTAAATATTTAATGTATTTAGCTTCTTCTTTTTTATAGTGTTCTGATTCTTCTTTTGTAGGATCAACAATTACTTCGCCTTTGATTCCATCAATAATAATTGTTTGACCATTTTTAACTGATTCTAAAACATTGTTTGTTCCAACTACTGAAGGAATGTTCATGCTTCTAGCCATAATTGCTGTATGACTTGTTGGTCCACCAATGTTGGTTGCAAAACCTTTAACATATTTTTTATTTAATTGAACAGTTTGAGAAGGACTTAAATCATAAGCCACAATAATTACTTCTTTATCAATAGCAGTTAAATCAGGTTCTTCAATGTCATTTAAAATGTAAAGAAGTTTTTTCATAACATCTTTAACATCAGCAGCTCTTTCTTTCATGTAGGCATCGTCCATATTTGAAAACATTGTTGCCATTTCTTCATAGAAAGCTTTTGTAGCATATTCTGCTGTTTCTTTATTGTCTTTGATTCTACCTGTAATTGTTTCTAAAGCTCAAGGGTCATTAATAATTTGTAAATGCGCATCAAAAATAGCAGCTTGTTCAGGAGTTTTAGCTAATTTTTTAGTAGCTTCAACTTTTTCACCAGCTTTTTTAACAGCATCATTATATAGTTTTAATTCATGATCTGCATCCTTAGCTTTTTCAGTAATATTGACTGGTAGTTCTTCAATTTTAAAAACTTTTGCAACCGCGATTCCACGACTTGCTCCGATTCCTTTAATTGTCATTTTGACCTCCATTTTATTTAAATATGTGTGTAATTTAATTATATAAACATAAAGTCTTTTATGGCTTTTTAACTATCTTAATAAAAATTAAATAGGTAATCATTTCCACAAGCAAAACACATTTTTTTTAAAAGCATTTTACATTTTAAATTTTTCATAAAAGCCTGTTTTATAGTTTTTTTAGTTTAAATTGTTAATTTTGAAAAAGTGAAAAATAAAAATCCGCTTACGCGAATTATTATCAGATTTTGACTCTTTTTTCAGGTGCTAAATACATCTTATCACTAGGTTTTGTTTTAAATGTTACATGAAATTCATCTAAGTTTTTAGCTTGAATATTTGCTCTTAAATCCTTAGGCGCATGAACATCAGCAGCTAAAAGCATTTTAGCATATTCAGGTTTTTGTTTAGCTCTTCAAACTGTAGCTCAGTTTGTAAAGAATTCTTTTGATGAATGATTTAATTCTTTTTGACTAGCTTCTAATGCACATCTAATTCCACCAGCATCAGCAATATTTTCAGATACAGTTAATTTACCATTACATTTACCAAATTCTGAATCTTGGCCTTCAAAAAGATCAACCATAGCTTGTGAAAGCTTATTGAATTTTTTGAAATCTTCATCTTTTCATCATGAATTTAAATTACCTTTTTCATCAAATTTAGCTCCATTATTATCAAAAGCATGTGAAATTTCGTGAGCAATAACAGCTCCAATTCCACCATAGTTAGCTGAAGAACTTTGGGTTAATGAGTAGAATGGTTTTGCTAAAATCCCAGCAGGGAAAACTATATGATTCATAAAAGGATTATAGTAAGCATTCACTTCAGCAGGTGACATACCTCATAAATTTTTATTAATTGGTTGTTTGTATTGACTAAAGTTATATTCGCTTTCAATTGCATTAAGATTTAATGCATTAACTACTAAATTACCTTCATGTTTATATGGAGTTATAACCATTAATTCATAAAAAGGTCTAAGTTCACTAGGATAGCCAACATGAACACCTAAAGCATTTAATTTAACAATGGCTTTATCTTTAGTTTCTTGTGAAAGTCAGCTGTTGTTTCTTAAACGGTCTTGATAAATTTTGATCATTTTTTGAACCATATATTCAACATTTCTTTTAGCTTCAGGACCAAAATTTTCAAGTGCAAATCTTAAACCAAAAGGAATTTTAAAGTATGAATAAGCTAAGTAGAAAGCACTTTTAGCTGCACTTGAAGCTTTATCAATTCCACTTACTGCACGGTTAAATTCTCCTGCAGTAATTCTAGTTTTATTGTCCAAAAGATTTGAAAATCTAATTAAGTTTCTAAGATACATTCAGCATTTAAATGTTTCAAATGTTCTTGAGTTATATAAGCTATTTAAACCTTTCATAAATTTAGGATAAATTACAACAACTTTTTCGCTAACTTCTTGTTTAATTAATCTTTCTGCTATTTCTTTAAGATCAAAAACTTTTGAATGTTCATTAATTTCATCAATTGTAAAAGGATTATATAAAGAGGGATAGTCAGCACTTTCAACTGCACTCATTGTGTATTTAACTAATGAGTTATCAAATTCTAATGTATTAGAAATAATATCTTCAATTTTATTTTTATCTTTAAAATAAGCAGCTAATAATTTTCTTGACATGTTTTTAAATACTTCTAAAAGCGGTTGTTTAGCTGGATGATTGTCATCATAATATGATTTTTCTGGCAAAATTAAACTTGGTCCACTAAAACCTAAAGTTTGTAATTCGGTATTTTTAAAATCTTGCATTACTTCAAAATCAAAAGGCAAGTGGAATTTTCTTAATTGATAAAAATCAAAGTTTTCAATTAAATCATCTCATGATTGCATTTCTCAAATTTTATTTAAGTAAGGTTTAAGTGGTTTAACTTTAGATTTATTTCTAAAAGTAAAATCACTAGCCATTGAATAAAGATTTGTGAAGTTTAAAATTGCTCCAACTTCTAATTCACCTGCATCACGAGTTCTAACTAAATCACTAGCCATCTTTCTTAAAATTCTTTCATTGCGGTTGTGAATTTCTAAAAATGCTCCTGTAGCACTTTTATCTCCAGGAATTTTTGCTTTTTCTAATCATTTTTGATTGACTGCAAAATATAAATCATCTTTAATGTTATTTTTTTTCATTAGTTTCTCCATTTCTATATCTAATATTATATTTAATAAAAGATTTTAATTAATATATTTGCTTTATTATTTTTCTTTGATATTAGCTAGCATATACAAACAAGCTGTTTCAGCACGTAAGATTGTTTTGCCTAATGAAGTAATTTTAATATTGTTTTCTTTAGCTAATTCTATTTCTTCAGGACTAAAACCACCTTCAGGTCCAACTATTAAAATTGAACTAGTTGGATAAGTGATAATGTTATTTTTTTGGTCTTGATTTTCATAAGCTAAGTACTTTTGCATAGTAGGATAAGTCTTAATAATTTCTTCTAAATTCATAATTTTAGTTAAATAAGGAATGATGTTTCTAAAGCTTTGTTCAGCTGCATTTTTAATGATTTCATTAAACCTATTTTGCTTACTGTTGAATTCATATTTTAAGATTTTTGCATCAGTATATTTAGAGGCAAAAGGAATAATTGTAGTAACTCCTAATTCAGTAGCTTTTTGCAAAATTCATTCGAATCTTTTAATTTTAATAATTGGTGCAGCAAGAATAATTGGCTTATTAAATTCATGATTTATATCTAATTTTTCGATAATTAAAGCTTTATTATTTTCTAATTTGCACTTATAAAAAATGTCTTGATAGTTACATAAAAATTCATCTTTTTCAACACGTGCAACTTTGATGTGATTTAATATCTCTTTTGATAGTTCAAAATGATCTTGATCACTTTTTTTAGTAACAAAAAATCTATGCATTTTATTCCTTTCATTTAACTAAAACAACTGTTTTAAAGCAATTTAAGTAAAATTATATTTTTAATTTTTAAATTAATTTATATTAATATTTTAGCAAAAAAATAAAAAGAGGATTAAGATAACCCCCTTCTTTTCTATTAATTTATAAAATACAACTTTAACTATTAATGATTTTCATCAAAGAAATTTGGCATTACATCTTCATTTTCATCTTCATAGTTTTGAGCAGTATAACTCATTATACGAGTATGATGTGATCCATTAATTTGTGTATATGTATTTTGCATATTTTGCTCATAATGTTCACTAACATTTCTTTGCAATAATTTTGGTACCGCATAAGTATTTGTTGAAACATTTGCATTAAGATTTGAAGCAATAATACTAATTCTGAATATTTCTTCACGATCTTGAGTATCGATATATTGAAGTCCGATAAAAGTTTGAGCTTCAAGGTTATTTGCTAAATACTCATCAATTCGATTTTTTGCTGTTTCAATGTCATCTAAACTTGCTCTTTTATCACACTGAAATAAAATAGCAACTTTTTGACAATTTTTAATTTCACTTTGAAAAAGTGGAGTCATTAAAGCATCATCAACTGCTTTAATAGCTCGGTCTTTGCCAAAGCCATTACCAATTCCTATAATTGTTTCTTTTCCATCTAATAAAACTTGACGTAAATCATTGAAATCTAAATTAATAAACCCAGTTTCAAAGATAATATCACGAATTATTTTGATTGAATTTTTCAATGTATAGTTAGAAACTTTAAAAGCTTCTTGAACTGGTAAGTTTCTGTAATTTTCAACCAATTTTTGGTTTGAAATTACAATATAACTATCAACAACTTCACTTAATTTTTTCAAGCCTTCTTGAGCAATTAAGCTTTTATGTTTTCCTTCAATTAATTCAAAAGGAGTGGTTACTACTGCCACTGTCAAAATGCCCATCTTTTTGGCAGCTTCGGCAATCACTGGTGCTGCTCCAGTTCCTGTTCCGCCGCCTAAGCCAGCTGAAATAATAACAATATCTGTGTTTTCTAATGATTTTTGAATTTCTTTAATGCTTTCTAGTGCGCACTCACGTCCTACTTTAGGGTCCCCACCAGCACCAAAGCCTCTGGTAGAATCACCTAAAAGTATTTTATTTGGACATAAGCTTTTAACCAAATCTTGACGATCTGAGTTAGCAACTAGTAATTCAACATTTGGCAAGTTTTCATCAAGCATCAAGTTAATGGCGTTATTACCTGCACCACCAACACCAATGACTTTTAGTTTAACTTCCAAATCTTCAGCATTTAAGTCCATAGGCAACCTCCTTATATTGTTTTATTTAATTTTGTCTTATTTTTGAGCTCTTTGTGATGGCAATATTCATGAGAATAATTTGTTTCAAAAACTTTTTGGTTTGTATTCTGGTAAATTATTCAAAGTATTAACCATGTCTTCACAATTGATTTTTTCAGGATTTAGTAAATTAATAATGCCTAAAAGGCTATTAGGTATATTGAATAAAGTTGAGACGTTATCGTCTAGAATTGCAACATTCATTTCGGGATATAATTTGTTAAAACGATCCATAAAGATTTCTGAGTTTTTGCAGTTTAAAACCAACTCATCAACTTTTTGATTATTTTGGTCTAAAAACGCTTTAACTTTATGAATAGCTTGGTCAAATAAAGAAATCACTAGTCTATAAGCTTTATAGCCTTCAGTTTCATCATTTAAAACTTGGTAAACTTTTCAATTAGCTACCACAGCTTCAAGTAACAAAATTGTATCTTCTTTACTTTTATTTAATTCTTTTGATAAATAATTAACAAAGTTTTTAAAGTCAATGTTAGATTTTTCATAGAAAAATGTTTTAGCATTCATAGAGCCATTTACTACTAGTGCATTTCAGTCAAAGTGGAATAAGTATTTAGCTTTATCTTTTTCTTCTAAATTTGCACTAAGAGCTTGAGATTCTAAAAGTAAAGCATCAAGTTTCAAATTACATTCTTTAATAATGTTAATAATTTTTGCAAGTCAAGTATCTTTTGTAATAACAAAAAGTCCTTGATTAATATTTAATTTTAGAGCTTGTTTGTTTCAAGGAAAAGTACTGTAGCTTTTAACAACATCATTGTTAATTGTTTCATATAAATATATGTTTGAATTAATCAATTCGTATGTTGAACTTTCTTTTTGTTCTTCAAGTTTATTTTTAACTTCTTTTCATACTTGTTGATCAACTGTATTACTTGGAACATCAAAAGTCAAAGTTTGGTTAACAAATTTTAAACTTTTGTATAAACTATCTTTAACTATAAAAGAATATTTAACTACATCATTTTTCTTGGTAGCAACTTTAGATTTCAAATATTTTTTGAAATTCGTAATAAATTCTTTAATCTTATTCGCTTCAAAGTTTTTGGTTTCTAGATATGTTTGAAAAACTGGAAAATATTTAGAATTAATATCTTCCACTACTGTCACATCAAGTCTAGTTTTTTTAAGAAAGAAATTTACATAAATTTTTTTCATAATTACCTCCAAAATAGTCTTTTTAGCAAAAAAAGATAGATTAAAAAAATTAAAAGCATTTTTTCAAAACTCTTAACTTGGCGCTACGAGAGCGTTTATTAAGTTCTAACTCATTATTGGTTGGAAGAAATACTTTTACTTTATATTGCTTCTCTTCATTAATTGGCATTTTTGCTGGTAATTTACTTTTAATTAGTGAACCAAAAAAATGTTTAACAATGCTGTCTTCCAATGAATGAAAAGTTATAACGGCTAAAGAAGTATTAGGTTTTAATAGTTCAATAGCATCTTGTAACATTTGCTTAAGGGAATCAAATTCATTATTAACTTCAATACGGATAGCTTGGAACACTGCTTTATTAGGATTTTTTAGTTTCACTAATTTTGCAGGATAAGCATCTCGCACTACTTGAGCTAATTCCAAAGTGGTAGTTATTGGCCGCTTACTAATGATGGCCTTCGCAACCTGACGAGCAAGTTTTACCTCAGCGTAGTTTCTTAATAATTGTTCCAATTGACTTTCACTATAATTATTAACTACATAGTAGGCATCAAGCTTTTGACTTTGATCCATGCGCATGTCAAGACGTGCATTTTTGTTATAAGAGAAACCTCGTTCATCATTATCAACTTGTGGACTGCTAATACCTAAATCAGCAATGATTCCATCTACTTGGCTAATGTTCAATTTATCTAATTCACTTTTTATATTTTTGAAGTCAGAATGAATTAGTTGAAAATTAGTTGATTTTATTTGGCTGAGACGTTTGCGACTTTCTTCTATCGCAAAAGTGTCCTTATCAAAAGCAATCAAACGACCAGTGTCAAGTCTCTTTAAAATCTCGCTAGAATGTCCACCCATGCCCAAAGTTAAATCAACATAAATACCATCTGGTTTTATATTAAGGGCATCAATAGACTCTTGCAGTAAAACAGAAAAGTGTTGATTCTCCATTTTATTTACCACTTCTTTCAAGAATTTTTTGTGCTGCTGAAGATATGTCACTGTCATTAAAGTTTTGACAATATTGATCAAATTTTTCGCTGCCTCAAAGTTCTACAAGATTACCTGAGGCTACAAAAATGGCTTCTTTTTGGATAGCAGCTTTGTCGAGGAATTGTTTTGGAACAACGAAACGTCCTTGGTTGTCAAGTTCAATTTCTTGAGTATTGCTCAATCAAAATCTTCTAACAAAACGAGCATTTGTATCAAAAGAGTTTTGTTGATTTAGAACTTCAACGAATTTATTGAATTCTTTTTCGCTTCTAATTTCAACAATGCCTTCAAGTCCGACAGTTAAATAAATTTTGCTTCCTAAATCGTCACGCAATTTAGATGGTAAAGCTAATCTTTTTTTGTCGTCAATGTTTCTTTTGTATTGTCCAAACATTTTCCACTTCCTTCCACTATCCACCACTATTTTACACTATATTAAATACATGTGCAAGAAAATATTTGAATTTTTTCATTAATTTAAACAGCTTTTGCAAGCAACTTTTGAAGGGGCTATAACTAAAAAATGCGTTTTTCAGCGAATGTTCGCTGAAAAACGCATTTATCGAGTATAAAACCTTCAAAAATTTTGTGAAAATTAGGCAAAAAATTATTTTTTAGAATATGTGGAGGACTTTCCCTAGTCTTCGACATTTAAGAAACCAAAAGCATTGGTTACATCTTCAACCTTACCTAGAATTGTAATAAGATCACTTTTTTGTAAAACAGTTACCCCAGTAGGTAAAATACTTTCAGTTCCTCTCTTAATTAAAACAACTGAAATTCCTAAGTTAAAGAATTTTAATTCTTTGATTGGTTTATTGATTAATTTTGGATTATTTAAAATAGTAGTACCCATAACGAAGCCATCGCCAATTTCTTGTAATGTTTGAGAATAACGGCTAAAATTTGGATTAGCTGCTAAAAGAGCAGTACGAACACCGGCTTCATATTCAGGTCTAACAATAACATTAACTCCAATTTGTTTTAATACACGTGCATGTCTTTTACTTGTTGCACGAGCAATAATATTATTAACTTTTAATTCGAGTAAAGCAGCCACAATTTCAATATTATCAGGAACAGCAACAACAACAGTTCCAATTTCTTGAAGATTAAGTGCTCTTAAAGCTTTCATATCACCTGCATCAGCAATAACAACTTTTTGTGCTAAATCAGCATATGATTTTAAAACTTCTTCATCTTTATCTATTAATAATAAAGGACAATTCATTTTAGCAAGTTGGCTTACAACCGCACTACCAAAACGCCCTGTTCCAATAACACAGATATATTCATTTTTTCTTTTACGAGGCATAATTTACTCCTTTGTGTTTTTATTAACTTATGCTATAAATTATAAAAGATTTTTATTTTTTTATAACTAATATTTTAACTCCAAAAATTTGGCAAACTTTTTTAAATAAAAAAGAAAATAGTGCTCTATTTATTAATAATAAATAAATTTCATAATTTTAAAATTAAGTTAAAATATATGTTATGAAAAATTCAAAAATTGCCGCTTGATGAAGAAAAAGCAAAATTAGACAATTTTTTGCAAACTATAGAGTTTGAAGATTAAATATCACCAAAGTTAAATACATCTTTTTAGCTTATCTTCTTACTGTTATTATTGCAACACCTTTCCTTTTAAGTCCTTGAACTCATAAAGATAGTAGTGTTAAAGTTAGTTTTTGAGATGCTCTTTTTACAACATCTAGTGCTTTCAGTGATACAGGCTTAGTCACTAAGACAACCTATAATACTTGAAATATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGAATAATTTTTAGTTTTGGTTTAACTTTCTATTTCTATTATAATGAACCAAAAGCTTATAGCGATTTTGATAAATATGGAGATTATATTTCACCTTATAAAAATTGGGGCCTAAGTTTTAGATATGGTTTCTTCCATTGTATTAGTGCCTTAAATAATGCAGGCTTTGACATTATTGGTAAAAATTCACTTATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGAAGACTATTTACAAAAATTAGTTTATTAACTTACTTTGTCACAACTTTAGTAGCCTTTATCTTAATTATTTCCTTTGAAGGTACTTTTAAAAATGATAATTATTTTTGAAGAAGTGATGATAAGAATTTAGGCAATAAATGAGAAAAAGCTTGAGCATTATTATTTACAACTTTTTCAACTAGATCAGCAGGATTTTCAACTATTAATTTTAGTAAACTAACCTCTACTTCATTAATCATTTGTTCAATTTTAATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGAGGTAAAAAGAAAAATTATTCTAACAAATATGAATATATTTCAGAGCCTGTAGCAATTGGATAAAATTATGCAAAATAATAATTTATTAGATTTAGGAATAAAAACAGAAAAACTTGAAAGATGAGCAAGCTATAGCACAAATAAAAAATATAGAATATTAGTTTCAGTTTTTTCAACCTTTTTATTACTAACAATAGTTTTATGTTTAATCTTTATTTTTATCTTTAAACATGAAACTAAAGTCTTAATTTCTTTAAGTATTGTTGCTTCAATAGCTTTAATAATTTGATTCTTATTTTTAGCTCCTTTTACTTACTTAATGATCACTTCATTTTGAACCTATAGAGCTATAAAACAGCCAGATAAACCAATTTATAGAAATTATAAAGAAGCTAATTGGTGAATTAAAATACAATTAAATTATGCTAATTTTGGCTTTAAAATATTTAACAAGAAAGCTTTGCATTTAACTAAAGAAGAATACAAGCTTTTTGTCAATTTTTACATGAATGTAAAATAATAGTTTTTATATTTTGCATTCTTTTTTATATAATCTAATTATTATGAAATTAAATTTATTTGCGTCTGAATACAAATCATTGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGATTAAAGATGATTAATGAATGAAATAATTTAAAAAAACACGATTAGCATAATCTTACTAACGTGTTTTTTTATCATTATTTTTTATTGCTTAAATAGTCAACAAATTGTTTTGTTATAAAAGTTGGTCTAGTAATTGCTGAGCCAACAACTACACCATTTGCACCTATTTTATAGGCTTTTTGGACCATTTCAGGTGTATTAAATCCGCCTTCAGCAATTATGTATTTTTTGTACTTTTTATTTAATTCAACTACTTCTTTTAGAAATTCAAAATCATTGTAACTATTAGGTTTATTTAATGTGTCTTTTGTGTAGCCTCTAAGAGTAGTTGAAACAATATCAAAATCTAATTTAAAAGCATTTTCAACATCTTCAATTGAAGAGCAATCTGCCATTAATAACTGATTATGATTATTTTTCTTGAAATATTGAACTAAGTCTTCCAAACTTTCTTTTGGTCTTTTTCTTAAAGTTGCATCTATTGCAATTATTGGACATTTACTTTCAATTAAGAATTTTAAATCATCTAAATCAGGAGTAATAAATACTTCAGAATCTTTAGACATTTTTTTAGTAATACCAATAACAGGAATGTCTTTAGGTACTATTCTTAAGACTGGTTCAATATTTTCTCTTTGGCTAATTCTTATGCCTTGAGCACCACCTAATAAAACAGACTTAACCATTCTTTCGATTACACCAGGACCATACATTGGCTCATTTTCCAATGCTTGACAAGAAACTATAAACAAATTATTTTTAAGACTCATCACTTAATTTTTCGTTAATTTTACTTGCAACTACAGTTGCTTTTCCGCCAAGTATTACTTGAACTGATAAATTATCATGAACTAAACCAAATGATCCATATGGTACAAAATCTTCTGCTTTAATATCAACATTTTCTTTTAATTCAATTCTTAATCTAGTTGCACAAGCAGAAACATTGTTTAAGTTTTTAAGACCGCCTAATTTATTAACAATACCTTCAATATTAGCATCGTCAAATACTTTTTCTCCGCCTGCTTGTTCAATTAATTTTCTATCAGCTTCTTGTTGTTTTAATTCTTGATATTGTTTTTTATTAATTAAACCAATATTATTTCCAAGACGACCTGGAGTTGCGATTTTGCCATATTTAATTCAGAAGAAGAATGTTACAAAGGCAACACCTCCTAAAACAAGAGCTCATAAGAATGCAAAGTAGAATGCAGTACCTTTAGCAACTGGAATAACACCATAAATAATTAAGTCAATGAAACCACGAGCAAAACCAACACCAACGTGAGCTCCTGTTCATTCCATTAATGCATATGCTAAACCACTAAGTGGTACATAAATAGCATAATAAAGTTGAGGAGCAATAAACAAGAATGTAAATTCAAGAGGTTCTGTAATACCTGTTAAGAATGCAACACCCATTGCTGAACCTATTGTTATCGCTACATTTTTACGATTTTCTTTTTCAGCTAAAAGAATCATAGCTAAACCAATACCCATACATGTTCCAATGTAGGTTGGATAGTCTTGGGTAAATCTTCCGGCATTAACTCCAAGAGTTTTTTGGAATCAAGGGAATAATTTAATATTATCAATGCTATTGTAATTTAATTGATTAATGTAGTTTCAGATGTTTTGGTCACCAGTAATAACATCTTTTCCTTTAGAAAAACTCTCTAATGAATTTTTTAAATCAGTAGCTACTTTTGAAGAAATTTTACCAGCTGTTACAGCCGCATCAATCATTTCCTTAGATAACACACCACCAAATTCAGTTTGATATGCTAAAACAATTGGAATGTGGTGTAAACCAAAAGGCATTAAAGCACGGCCTAATATACCATAAATAAATCCATCTAAACCTATTGGTGATCTTTGCATTAGTTTACCTAATTCATAAATTGCTAAACCAATTCATGGTCATAATAATAAGAATACAATAGATACACCAAAAGAAATAGGTACTAAAATAATTGGCACTAATCTAATTCCTGAGAAGAATGAAAGTGCAGGTGGTAATTCAATTTTAGAGAAATAATTAAATGTATATGCAGTGACAATTCCAATAACCATACCACCAAAAATACTTGTATTTAATGATTTCATTCCTAAGTTACTTCCTACAAGTCCAGCAACTGGTTTGTGGAATCAAAAAATACTTTTAACAATATCAACACCATCAAATTGGATGAATGCTAATTGAGAACTAGTAAAGACAAGGTAAGCAATAGCAGCCGAGAATCCTGCTCCACCTTTGTCTTTTGAAAATGTGATCGCTATGGCAATACAGAATAAAATAGGTAAGTTTCCAAAAATAACTTCAGATACACCTTTGAAAATATTAGCAGTTGTTTGACCAGCACTATTAGTTGTATTTGCACCAATAGCTCCACCGATTCCTAATAATAAACCAGCAATAGGTAAAATTGCAATAGGCAACATTAAACCTCTAGCTAGTTGTTGAAATCAATCTTTGATTTTTCCTTTTTTAGGATTGCTCTTAATTTCTTTATTAGGAATTTTAGCTTGAGCTTTATCCATAAGCTAACCTCCATATTATTGAAAAATGAATTTTTTTCACTTTTCACCATAATTATATTTAGATATAAATATAAGAATATTAATTTGTAAAATCCTTACAAAAGAATACCAAAATTTTGTATATTTTTTTCACCCTAAAAGACAAATTTAAAGGATTTTAAGACATTTTTTACACTTATTTTGCATTCATTTTTTATTAGTATAAAATTAGATAAGATAAGGAAGTGCAACATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGGTTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGGTCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAGACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATAGTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAAAAAGCGATAAAAATTGAAAATAAGAAGCAACAAAAAACAAGAAAAAAATATTTTTGTAAAAGTCTTGTTTTTTTGTTTTTTTATTATAAAATAATAATGAATTTTTATAAATTCAAATTTGATCAAATTTAAATTAAACACACTATTATGGATTCACTTGGGTGTGCCTGGAGAAAGGGTGCTAGGTGTTAGAAATAATAGGAAGTATAACAAACTAAAGGAAAATTTTATATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGAAATCCTCGTATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAATAAATAGCTACTATGTTACTGAAAGATGACTTGGTGGTACATTAACTAATTCTTCAACAATTTTCAAAAGAGTTAAAACTATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAGGAGTTACTATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGAGACATTGAAAAAGCTATTACATGATTAAAAAGTAATGGAAAAATTAAAGCTGCTAAAAAAGCTAACAGAGTTTCAGCTGAAGGTTTAGTTGCTATTGCAGCTAATGCTACTAAAGCTGTTATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAGCTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAATTATTTTTTAAAGTTACCATTTGACTTTTCAAATGGTACTTTTTTTGTTTTTAAAAATTATTTTAAAATCAAAAAAGTTATACACAAACAATCAACATAAAAATTGATATTGGATTTTGACCAATTAAACTTATTTAAAGGTCAAAAAGTAAAATAGACAAAAACTTATGCACAATTTTCAAATTAATACTTTTTTGGAAAAATGGAATCATTTTCTTTTATACAATTAAATCATGGAAACAAAAATAACGACGAGTAACGAACAGTTACCAATATTGAAACCTATTTTATTAGCATCAGATTTCGTCGAAAAGATCAAAGGAGATAAGTTATATTCAGATGTGATGATGCTAATAGCACAAGATGAGGATTATGTCTATTTTGTCTCATGTTTAAAAGAATGAGAAAAGAATTATGAATTGGAACCTCGTTTTATTAAAATATATGAGGAGGAAGGCATAATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAATTTTTCTATACACTACGATATTTTGAATATCGCTTTTGGTATTGGGCGATATTTTTTTGTAAAAAAATGAGCCTAATTCAGACTCATAATTCTATTATTCGTATTGGTGCATATCAACATTAAATTTACTCATTACTTCATAAATTTCATCAGCATTTTTGGCTTTCATAATTTTATTATCCAAAGTATTATCTAAAGCTAAAATACTAATCATTTGTAAAGTATCAATTTGTAAATTACGATTATTTTTACTTAATACTAAAGCAAAAACTAAATTAACATTCTTTTCATCATCAGCTTTTCAATCATTAACCCCATCATTATTCATAGTTAAAATACATAATGTGTCTTTTTTAACTGTTGAGCTTAATGCTTGACAAATTGCAATGCCACCGCCTATTGCAGTATTCATTAATTCTTCTCTATATAAAAAAGCTTCATATATTTTCTTTTCATCACTAACAAAGTTTTGTTCATTTAGTTGATGTGATAAAAAGTGCAAAACATCATCTTTTTTATCAAAATTTGAATGTACAAAAATAGAATTTTTGTTTAAAAAATTATTGAATTTCATTTTAGTTGTTCTTTCTATTATTAAATTTCTCTTTTTTCTAATCTAGGCATGATACTTAAAGAGAAAGCTTCAGGACCTGTATGAACTGCGATGGCAGAAGATGTTCATCTTTCTGTATCTATTTTAATATTTTTTTCTTTTGAAACTTTACGAACAGTTTTATTAACTTCTTCATCAATTCCGTGAATTCAGTCAATTTTGAAGTTTTCTGTTAATGTTTTGAGATCTTTTGCTTTAACGAAAGCTCCTAATTTTTTAAATATTTGAATTATTGCACCCTTAACACCACGAGCTATTCCAGCTGTTGAAACATGTTGTTTAATAAACTTAACATAAGGATAAATTCTTAATTTAGACATTGTTTTTAAAACGAATTTTTTAAAGCTATTATTAACTCTACCGCCCTCAATCAAATAATTAAGTTTTATTGGCAAAACGTAAATTATTGAATTGTTTTTTATTCATTCTAATTCTTCAAATACTTTGTCTAGTGTTTGATATTTTTCATAAACTTTTTTAATATGATTAATAATTTCATTGTATTGGTCACCAACTCAGCAGTTATCATAAACGTGAAAGTTTTTATATTCTTTGCAAATTGTTGCAATGTTGTTGCAACTTGAAGATAATGATGAACCAATTGGGAAGTAAATTACTTCATCATATTCTTTAGTCATTTTGTCCACTGTATCTAAAACAGCTTTAACATTTGGCATAGATGATTTAACTGATTTTCCTTGATCAAGTTTATGCAATAAATCTTCTTGGTCTAAGTCAATACCATCTTTATAAACTACACCATCAATATCTGTTAAAAAAGGAAAATAACCATAACCTTTTTTAAGAATTAGTTCTTGCGGTTCACTAATAAAGCCGTCAACAATAAAACCTATTTTTTTCATTATTGACCTCCAGTCTTTAAATAAAAATACTTATCTATTATAATATACAATTATAAAATTTTATAAATAATTTTAATATCATTATATCATTAATTTTTATGCATTTTTATTTTAATTTTTTAACATTTTTAATTTAGACTAAAATGCCTATAAAACAATAAAAAAAGAAAAAACACATGGTTCATCTTTTTTCTATTTGCAAACATATTGTACATCTAATGTTTTTACCATAAGTTCATCTAAGCCTTCTGGTAAGTTTTGATAAATAATAACATGATTTTTAATAGTGCTAATTGTTTTAACGAAGTTTAAAAGTTTTATGTATAAAATTTGATCTTTTTCAATGTATTTGCAAATCTTTTCCGATATAACAAAAACCTTGATATTTCCGATATTAACATAGTTAGTTAAACGGTCATTTATCTTTTCGATATATAAACCTAAGTTAATGCCTTCTTTCAAATTACTAAAAGCATTGTTAATTAATCGATAATTAAATCCGTTTTGTAATGGTTTAATTAATAAAGTTGTTTTTGGTCGATCATATGTATTTAAATTAATTAAGTCATATTCATTTGTTTCCAAAATAATGTTTTCATCTTCTTCACAATTTTCCATTAAATATTTGTTTCATTTATTGGTATAAATCAAACGATATCACGAAACATTATTAAAGAATTCTGAATCTTGGTCAGAGTAACCTATGACTCTATTTTTTAAAATTGCAACTTTGCTTTGACGTTTAGTTTTATATGAAATAATTGGAATGTTTTCTTTTATATATTGTTGCAATTCATTTTTCTTTTCATAATCAAGCATCATAGATTTAAATTCTTGATAATGGTTGTCATGTTTTGTATCTGAAATTAATCAAGAAGAATATTTATCAAATTTGCGTTTGTTATTTAAGTGTTGTATTAAGTATTTTGATTTATCAACTAATTCTTGAACATCATCAACACTTTTGAAAGTGTTAACACTTAAAAAAGCAAAGCTATTGAAAAATGGATTAATAATTTTTCTTTCATGTAAGAATTTTAATCTTTTAAGTGCTTTTTTAGTGTAGTGGTTTAGCCAGAATGACGAATTGGTTTCAAAGATTAATAATATTAATCCGTTTTTAACGTAGTAATATCCAAACATATTATTAAGCTCAAATATTCTCAAAATATCTTCAATGTCTGATTTACTAATGACATTATTGTAGAGAAATTCATTGATGCCCAAAGCATAAGCCAAAATATATTTTTCCTTATTTAAAGCATTATATAAATCTTCAGATTTTTTAATAAATTTAAATGTTGTTGAAGCACTTCTTTTTGGTCTTGCGTTTCAGTGGATTGTTAAGTAGTATCGATTATTTCCAAGAGGGAAGAATTTTAAAAGAATTTTTTCTTTAGTCATTTTAATGTTTAGAAGTTTTAAACCTTGAGCATTTTGATGTGTTCGATAGCCTTTAGAACTCATCTTGCAGTAAATATTAAATCTTTTATCATTATTGTCGTTTTGTGAAAGATATTCAGTCAAGTTTTTTTGGCTCTCTTCATCTAAGTAAGAAAGAAAGACATCAAAGTCGACATATTGATTAGCTGAAAGACCTTGAAGACGTGAGTCCATGTTTAAAGATCCACTTAAGCTTTTAGAACTCAAGCGAATTATTTTTTTTTGTCCAAAGTCTAATTTAAAGATTGCTAAACCATTTGTTTTGACTTTATGAACATGATTTAAGTAAATAAAAACTATAATAAAGATGTCTATTAAAATAACACATGCTATTATAATTGCTATTCCCAATAATAATTGATCCATAATGCTCCTTTTTACTAAAATAGCTGTTTTATAGGTTTATTTGTTAATCATCTGTATTTTTTAAAAGTCGAATTGAAGCGTGATTTTTAGCTAAAACTTTGATTGTTTTTTGTCCTGTGAATCTAACGCTAATTGTATCGTTTTCAACAGCCACAACTTGGCCTTCGCCAAAGAAGGTGTGGCTAATAATGTCACCTACTATAATTTTTTTGTTCAAAGCTTTTATTTGTTTTTCGTCAACTATTTCATCAAAATCAAGGCCAACAGAACCTGTTTTAAGAATAAAGTTTTTAAGTTCAATTCCCATTTCACGAATAAAACGTGATGGTTTCTTTTCTAAATCTGTACCAATTAAATATCCACGACTATCGCTAATAAATAATCTATTTCTTGCTCTGGTAATTGCCACATAAGCAAGTCTTCTTTCTTCTTCTAAAGCATCATCTGAGTTTGATTTTAATGATTTATAGTTAGGGAAAATTTGATCACTCATTCCCACTAAAAAGATATTGTCAAATTCTAATCCTTTAGCAGAGTGGACTGTCATAAGTGTTACATAATTTGTAAAGTTATCATATTCATCACCTGCTGAAAGTAGTGAAACTGATTCTAAGTATTCTTTAATACTCTTATTTGGATTCTTCTTTTCTCAAGTTACTATTGAGCGAATTAATTCAGCAACATTATCTTTAGCAGTGCCACGAAGTGAGGTTTCTTCTTCTATATGTTCATAGTAGCCAATTTCCTTTAAGAATTTATCTAAAACAACACTAATTGAGTTGCTTTTTAAAGCTTGACGATAAAGGTTGATTTTGTTAAGAAACTTAACAATGTTGTTTTTAACATTTAAAGGTACTGGCAATTCCTTGTAATATTTAATTAAATATGAATAGAGAGTTAATTTTTTATTATTTGCTGCTTCTTGTAATTTTTTAAGAGTTGCTTCTCCGATTTTACGGTTTGGAACATTAATTATTCTATTTAATGAAACATCAGAGCCATCATAGATAACTCTTAAAAATGCTAGAGCATTCTTAACTTCAGTTCTTTGGAAGAATTTTTGACCATTAAAAATCTTGTGGTTAATATTTTCATTAATTAATTCTTCTTCGAAAGGACGCGAATAATAATTTGAACGGTAAAAAATAGCAATATTTTTAAGCTGAATTTTTTGTTTCTTTAATTCATTAATTTTTTGGACCACTCATCTAGCTTCAGCTTCGACACTAAAAGCATGAATAAATTCTATTGGTGTTCCTTGTTCACGATTTGTTATTAAATCTTTACTAAAACGTTTTTTGTTGTGTTTGATTAATTTGTTAGCTGCATCTAAAATTGTTTTAGTTGAGCGGTAATTTGTATTTAAAATAATCGTTTTAGTATTCTTGAAATCCTTATCAAAATCAAGGATCAAATTAACATCAGCTCCTCTTCAACCATAAATAGTTTGGTCAGGATCACCAACAATTGTTAATTGAGCATTTGAAGATAAGTATTTAACAATATCATATTGGATAGTTGAAGTGTCTTGGAATTCATCAACTAAAACATATGAATATTTTTTACTTCAAATTTTTGCCACTTCAGGGAAGTTGTCAAATAATTCCTTAACTTTGATAATTAAGTCATCAAAATCGAGACATTTTTTGCTTGTTAAATATTGATTATATTTAGAATAAATTTTTCCAACGAGTGCATTAGATCTATGCTTTTCTTTAGCATCTAATTCTTTAACAATTTCGTCTTCACTAAAGCCTTGGTTCTTGGCTCATGAGAAAATCTTCATAACATTTTTGTATGAAACATCTTCATTTGAAATGCCTTCAGCTTTATAAAGTTGAGCAATAATTGTCTTCTTATCTGTTTCATCAATAATTTGGAAGTCATTACTTAAATTAACATGAATAGCTTCCTTCCTTAAAATCTTTGAGCTTAAAGAGTGGAAAGTACAAATATCGATTTGATTAGCTTCTTCGCCGACGTATTGTTTAACACGTTCGCTCATTTCGTTCGCAGCTTTATTAGTGAAAGTCACTGCTAAAATTGAACGTGGGCTAATTCCAAGTTCATTAATTAAATAAGCCACCTTCCTAGTTAAAACTCTAGTTTTACCACTTCCAGCTCCAGCGATGATTCTTAAAGGACCATCAAAATACATTAAAGCTTCTTTTTGTTCAGCATTTAATGAATTCAAAATTTCATCTCGTTTGCTCATATAAGTCTCCTCCTATTTTTTAATGTAAATTACAGCTTCATTATTACTTTTGAATGGTAACAGTTTGATTTCATCTAAATAGCTTGATTGTGAGTAATGTTTTAAGTATGTTACTCTGGTGTTTGTTGAACGATCAAAAAGTCCAAGTCTGGTTCTTAAAGCCATTTCCATAATGTAATTAATTAAGATTAAAGTGAATCAAAAGCCAGATAAAAATATGATTAGTTTTGAGGCAATTTGAAAACGATAATTGCCTTCATGTTTTTTAATTATGAATTGACTAAAGTCATTAAATTCATTTGGCATAACTAGTGTTATTGGGATTAAAATTATTAAAAATCAGAAAATTAAAGCAAATGCATTTCTTCTTAAAGGTAATAATTTTAAAGCTAAAGGAGTCATCTTTTTAGCAGGTATTATTTGTACTCTAAAAATCAGCATTCCAATTGTTCTACTACTATAAAACAATGGAATTAGTAAAAATAGCACAAGTAAAATAACTGTTGAAAATATAATAAAAGAATAAAAGTTAATTGCCTTGACAACACCTTTATGGACCATTAAAAAGTAATAATTTAAAATTGCTAAAGTAACAAAAAGTCCGAAGTCAAATAAATTAATTAATAGTCGATATCAAACATTAATATTTTTATGAAATTTCATTTAGAATCCTATAAATCATCCAAAATATCTTCTCATTTTTTAACTTTAGGTAGTATTTTTAAAAAGTTAGCTTCAGGATTATTTTTAATAACATAAATATTAGTATTAAAGAATAGACTATTGTCTAAATGTTCTTTTTCTAAGTATTTGTAAAGCTCATTAACAAAACGATCTGTACGTTTTTCATTGTAATTAATTCTGTCTTTAATAACATCTTTTGATCAGAATCTTAGTCTTCAGGTATTTAATAAAGCACCTAGAAAAATTTGCAGAAAATACATGCGAGCATAAGCTATTTCTTGCTCAAATTTTAAGTTATTAGTTTTAACATAGTTTTCAATTGCTTTAAATTGTTCAATAAATGTTTTAGGATTCAATCACATTGATGAAGGAATAAATTCGCGAACAATTCTTTTGTTTGTATAAAGATAACTTTGTGCTTTAAGTAGCATAAAATAGTTTAGTTCGATACAAAACTTACTATCATTAACAACAATAGAACGGTGTTTTAAAAATTGGTTAATTAAAGATTTTTTGAAAACTTTATTAAAGATAAAAGGATATGAATATGCTAAAACTTGATGTTCTTCTTGAATGTTATAAACTTTTTCTTCTTCTAATCTTGGGTTTGGTTTTCATTTAACTGTGCCAACCAATCTTGGACGATATTCTAAAACATCTGGATTAAATTTTTCAATATAGTTTGTTAATTCTTGTGCAAAGTAAGAACGCATTGAGTTGTCACTATTAATAATTGTGGCATAGTTTCCTTTAACTAAGTGAAAAGCACAAACTATATCACTTTGCATAGTTCTCTGACGATTATTAGTAATAAATTTTAAACGTGTGCCAAAGAATGCTAAATATCTTTCAATAATAGAATACATTTTAGCTGAAGGGTTTCTTAAAGCTAAAATTAATTCAAAATCTTGATTATTTTGATCTTTCATACCTTCTAAAATATTTTTTAAACTTTTAGCGCTATTTATTCCTGGTGAAATAATTGATAATTTCATTTTACTCCTTTACATTTTAATATTTCCAGATGTTCTTGGATATGGAATAACATCTCTAATATTGTCAACACCTGTGACATACATTACTAATCTTTCAAAACCAACTCCAAAACCGCTTGAACCACTATCGCCAAAACGACGCAAGTCTAAATATCATTGCAATTCTTCTTGACTGATTCCTACTTCTAAAGCTCTTTGAAGTAATTTGTCATATCTAACTTCTCTTTGGCTTCCACCTATTAATTCGCCAATTCCTGGCACTAATAAGTCAAAAGCTGCTACAGTTTCATTGTCATCATTTTGTTGCATATAGAATGCTTTAAAACTTTTTGGAAAATTAATTACAGCTACTGGTCCATTAAAGATTTTTTCTGAAATATATCTTTCATGTTCAGTTGCTAAATCAAGTCCGAATTGAATATCTTGATTTTCAAATTGGTCTTTAACTTTAGCCAATTCTTTAATTGCGTCGCGATAGTCAATAACACTAAGTTTTGTTTTTAAAAAGTGATTTAATCTTTCTAAAAGACCTTTGTCAACTGTTTCATTTAAAAATTTAAATTCAGCAGGATGTTTTTTAATTGTATTTTTGATAACAGATTTTAACATGTCATCAGCTAATTTGATTGCATCCTTTAATTCATAGAAAGCAACTTCAGGCTCAATCATTCAAAATTCTGCTGCATGTTTTTTGGTATTTGAGTGTTCTGCTCTAAAAGTAGGTGCAAAAGTATAAACTTTTTTAAATCCAATTGCATAACTTTCAGCATGCAATTGTCCTGTAACTCCTAAAGTAGCTTTTCTAGTCCCAAAAAAAGGATCTTTTGAATTTCCATCATTAACTACAAAAGTTTCTCCAGCTCCTTCACCATCATTGCTGGTAATAATAGGAGCATTGAAGTATAAGTAACCGTGTTCATCAAAATATTTATTAACTTCTTTAGCTAACGTTGATCTTATTAACATTGTAGCTCTTAAAAGATTTGTTCTATGTCTAACATGAGGAATATCTCTTAAGGTTTCTAAATTGATTCCTTGTTTTTGAATTGGAAAGTCTAAATCAGCATCTCTTAATAATTTAACTTTAGTAGCGACTAACTCTAAAGGTTGTTGAGCTTTAGGAGTTAAAGCTACTATTCCTTTAACTTCAACTGCAGCACCTAAATGAATTTGATCTAATTTGTCAAAGTCAAATTTTTCGCCTTTGCAAGTTACTTGTAATGATTGAACAGTTGAACCATCATTAATACTAATAAATCTAATTTTTTTGTTACCTCTATTTGATGTAACTCATGCTTTAATTTCTAAAGTTTTGTTTTTAAATTCTTTACTCTTCTCAAATAAACTTTTTATGTCACTTGTTGCCATATCCACCTCTAATTAATACTAAATTTATTTATTATTTATTAATAATAATTTTAGCATAGAATATTAATATTAATATTAAATGAGTTTAAAAAACTTCAAAAAGTTGGTTCTGATAACAAAAAAAATTTGCAAAATAATTTAATTTAAAAATTTATCTATATAATTGCATAGGTTTATATTTAAAAAATAATAATTATTTTTTGGCCAATAATACTGTTTTATAGGCTAATAAGCAATTTTAAGTACATTATTAATTTATATTTTATTTAATTTATGAAGGGACAAAAATATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGAATTTTAGCTTTAAGTGGTTTAGTATTTTTCATTGTAGTATATGCAAACAAAAATGCTAGAACTAACTTAGGACCTGACAACGGTTGAATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGATATGTTTACGTAATTTTCCAATTAGTAGCAATAGCTGTTTATGTTGTACAAACTTACTTATATTATCATTTCAAAAATAAAGCTAAAAACGTTCAAAGTGAACAAGAAGTTCAAATTGAAACTAATAACTAAAATTCAAAATGATTATGAGGCTTTTAAGCCTCATTTTTATTTTTAAAAAATTAATTTAGTATAAAATAAAAAAGATTAACGTAATAAAAAAATGGAGGCAATTATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGAATTTTTGCAGGTCTAAATATGCTTAAATTAAAAGCTACTAAAAGTCTAAATGTTGAAAATTTCGAATTTAGCCAAAATTACTTTTTATTTTGAGACAAGTTAGAAAAATCAAATACATTTTTACAATTAATGATTGATAATCCTAAATTAAAATATGAAGATCGTCTTTTTGCTTTCTTAATGGAAGGCAGTGTTAGCGATGGTGGATATTGAGAATGAGTATCAGGCTTAATTAAAAAATATGGAGCTGTTCCTAAAATCGTTATGCCTGAAACATTTAACTCAAGCAATACTCACGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGCGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAATCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAAGATAAAAAATTTATCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAACATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTGCTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAGTCACTTAATGTTACAATGGATGTAATTAAAAAAGCTGTTATTGCTTCATTAAAAGATAATGAGCCAGTTTGATTTGATTGTGATGTTGATGCTTATAGCGATCGTAAAGGTGGAATATTTGATATTGATCTTTATGAATATGAAGAAATGTTGAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCAAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGAGAAATTGAAAACTCATGAGGAGAAGATGTTGGTAAAAAAGGTTACTTCTCAATGAGTGATAGATGATTTGATGAATATGTTTTTGGAGTTATTGTAGATCCTAAATATGTAGATAAAGTTGCTCTTAAAGGTTTAGAAAAACCTGCTATTGAATTAGAGCCTTGAGATCCTTTATCATAATAAATTATTAGTATAAAAAAATAACCAATAAGGCTGTTTTATAGTTTAATTGGTTATTTTTTTAAGTTTGTCTTTTTTAAGTTTAATTGCTAGATAAATAGTAATTGAAATAAGTATTATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGACAATTAGTGGCATGTATAATCATTCTAGCCTCCTAAATTAAAACCATTAAAAAGGCAAAAGCAAATCCAAAAATTAATAATGAAGAGATAATATCAGTAAATGTATTAATTAATGAAGCTGATATTGAACTTGGATCTTTTTTAATTTTAGCTCAAATTATTGGAAATGCAGCACCTAGTAATTTTGAAATTGTTAAAGCTAATCATAAAGCAATTGAAGAACCTATTATTATTGCTCAATAAATCATTTTGTTAGTTGACATTAAATCTCTTGTAGCTGCAAAATAAATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGAATTCCGCTATTACTTGTAGCATTATTAATTACTGGTATAAGGGCTGCAAAAGAAATTGTTGCTAATGAAATTTCGGCTACATCTTTAATTTTTTTAGCAATAGTTCATGTTCCAAAATACACTATAATTTGGGTCACTGTGGCAAAAAGAAGTAGTAATATTAATCATAAAATTCTTGATTTAATTATTTTATATCATGGAGTTTTTAAGTATTCACTTTCATTAACTTCATGGCTACTAATACCTGCCATTTTGTATAAATCTTCTGTAGCTTCTTCTTTAATAACATCAATAACGTCGTCACTTGTAATCATTCCAACTAATCTTTTTTCATGGTTAACTACAGGTAATACTGACATATCGTGTTCACTAAAAACTTGGGCTGCTACTTCGGTTTTATCATGAGCTGAAACATAAGTTACAGGATTATAAATATCATCAATTTTAGCATTAGGATCAGCAAAAACTATTTCTTCTAGTGTTAAAGCACCAATTAGTTTATTTGTTTCATTAACTACGTAATAGTAATGCACTAATTCAGCATTTTTCTTATGATAATCACGTCTAATTTTATGTAAAGCTTGTTCGCAAGTATATGAATTACCAATTGAAGAAATGTCTATTGACATTATGCTTCCAACTTCATCATCTTCATAGCTTAAAAGTTTATTTAATTCAGCTCTTTTTTCTTGAGGTGTATAAGCTAAGATTTTGCTTGTGACATTCGAAGGTAATTCTTCTAAAACATCAGCTAGTTCATCACTTTGTAATTCTTGTAGTAGTTTCATACCTCAATCTTCTGTAAAACTTTGAGCTAAATTAGCTTGGACTTCTTCATCAAGGTAGCTAAATAATTCAGCAGCTTCACTAGTTTTAAGTAAAGGCAAAAAAGTTAGCTGTTCTTCTAACTGTAATTCATTTAATAAGTTTGCAACATCAGCCATTGGATTATTATCAATAAATTCTCTGGCTTTAGTTACATTTTTAGTTTTAATAATTTCATGTAGTTCGTTTTGCAAACTAATCTCTTGACTTTCGCTCATTTTAATCCTTTAAGTATTTAACTAGTTGTTCTTGGAATTGTTCAAGACGAATGTTGTAGAAAGTACCATTTTTAACAAATTTAACATCCCCTGTTTCTTCACTAACAATTATTGTGGTAGCATCACAAAGTTCACTAATTCCCATAGCAGCTCTATGACGAGCACCATATTGATTATCAATTGATTTTCTGGTGATTTTATAAAAAGTAGAAGCATAATAAATTTTATTATCTCTTATAATTACAGCTCCATCATGCAATGGACTATATTTATTGAATATTGAAATAAGTAAGCTACTACTGATATTAGCATTTAAAATTACCCCATCAGTTCTCAAATTGTCAATATTATCATTGTTTTCAATTGTAATTAAAGCTCCAATTTTATTTTTTGATAAATATTCAACAGCTTCACGTAGTTGGTTAATTAATCTAATTTGACTACTTTGACCTAATTTTTGGAAATGACTTCTAGCCATTTTGTTTTTAATTGCATTAACTATAAGTGGCATTATTGAAATTAAAATTAAAGTAACTAATAATGCAACAATTACATAAAAACCGATTTTAGATTCTAAACACATATTTTGCTCCTAAGCATAAACTCTTGAAATTAAAACAGCTAGTAGTGTGCAAAGTAAAATAATTAAAACTGATAAGCTAGCTCCTAAAACTTTAAGAATAATTTTTTGCTTTCTAACTCTAGGATCTTCTTTCTCATGTTTATTTACTAAACACATAAATTCTTGGCATTCACCAGTAATAGGTTTTTCATCTTCAATCAAAGCTGGATTAGCTAATTCAGCTTGATTTTCATTATAAAGTTTTAATAGTAATTCTCTTCTTTTTTGAATTCTTTCAGCACATGTTGCTAGATCTCTATCAATAATTGCCATATACACCTCTTTCAATTGATATTAAATTTAATACTAATAATTAATATTTTATATTATATTCCATTAAAGTGAAATATATTAGTTAGGAAGTAACTAATTTTATTCAACAGCTTCAATAATTAATTTAACTTTTGGTCATATTTTTAATTTAGTGGCATATTTTTATAACAAATTATAATTAGGTTTATTATTTTTATATTTTTTGATAATTTTGTTATTTTAAAAGTGAGACACTAAATTTTAGAGATCTCACTTTTAAAATAATAGAGAACAGCTATTTTTCATTAACAATATTTAATTTTTTTAATTTCAAATTTCTTTTATTTGTTATTTTCTTAGTAAATAATTTAAAATGTTTTTTAGCTTTGACAAAAATTGTTTTTAGATAAAAGAATTTAAAATATGTTTTTTCGCTATCAAATAATTTTTTGTTAATTTCGTCTATCTGTTTTTTGATATTCTTTATATATAATTGATTTTCTTCTGAATTTTCTTTTATAACGATAGAAATTGTGTAAATAAAATTATTTTTAAAATTCGATAATTTTTCAATTAAATATGAATTTGCATAAAAATGATTTTTTTCATAAAAAGGATCGATTTTTAATTTTTCTTTTTTTCTTTCATTTAAATACAAATCTTTTTTAAAAAATATTCTTACAAATGAAACTGTAAGATAAACAAAAGTTATTCAAAATAAAATTACTCATTTATTAAATGTTTTAAGAACTGGAATTTCATTATTTAAAAAAAGTGCTAAAATAATAGGCAATAAGAACAAAGCAAAATTTCAAAAACTATCTAAATAATAAAATGTCAATTTCAATTTTGTTTTTTCAATATTATTTATTTTTGTTCAAAACCAAATTTTTTTATTATATTTCGCGCCTAATATCTCATGTAAATCAATATTTGATTCATCATTTTTTTCACAATAAGCATCAAAAAAACTAAAAAATTCTATATCTTCACAACTATTCCTAATAAAATCTATAATTTTAAATTTGTTTTGTAATTTAATATTAAAATGTTCATTTTTCCATTGAAAAATAATAAATAAATTGTTTTTATTTTCTACCAAAATTTTTTTTACTATACTAATATCCAAAATAATTCTCGGAAATTCTTTAAATATAAAAAAATTCTTTTTTTGGAAACTTGTATATGCACTCTTTAATGTTTTTCATATTTTTCAATTGCAATTATTTTTATTGTCTGCAATTTTTATTGTTAATTCGTTGTTTTCTAAATTATTTTTTGATTCTAAATATAATTGATTGTCTTGTTTTAAAAAACTAAATTTTATTATTCAAAAAACTAAATTTAATATAAGTAAAACACTTAATATAAGTATAGCAATTGTTCTTAAAAAAATGTCATTCAAATAAATAAATACATTTCAAATGAGAACGGAAAAGATGAGCAAATTAGAAACTAATAGTAAAAATATATAAATTTTGAAGAAAAGATTGTAATTAATGTGAATTTTTGAATCTTTTTTAAGTGTAAAGATGATAGTTAGAATATATCTTGTAATAATAATTATTAACGATAATATTAACGATAATATTAAAGAAATTAAAATATATTTAGTATTAATCTCGCTTAAATTTTTCATAGTCTAATTATAAACTAATTGAATAAGTCGCCTTTACTTTTTATATAATTTGCACAAAATATCTATAAAAAAGGCTAATTGATAAATTATAAGTTGTAACTTATTATGGTTTAGTTGCCATTTTTTCTTGAGCAGTTTCTTTCAATTAAGTTTTTATTTTTAAGTTGATTAATAATTTTTTTAATATAGCCTTCACTTAAAGATAAAGCACTGCTTAATTGGTTTATTGTTATATTTGATTCTTTTTCTATAGTTTTAATTACTTTAATTTGATTATCATTTAAATCCTTTTTATTTTCATTTTTATTTATTCTTTTAAACTCTTTACGATTAAAAGGAATTTTTACTCAAATTGTATTTTCATCAATTTCAAATGCTTCTTCGTCATATTTTTTGACAATTAAAGGCACTCCATATCCAGTTTGTTCAACTAGATCTAATTGCATAAAAATTTTTTGTAATTTTTCATTTACTGGTCTACTTACGCCTTTGAAAAATTCCTCTTTAGTCATATTTCTAGAAATTCCACCATTTGATTCTATTTGAATATAGTCTTTGAATATATTAACTTGAGGTGGATATTTTTCATCTCAATGATTATGAATGCATGCATTTAGTCATGCTTCTCTAAAACACTCAAAATCGAATAATTTTTGTTTACTCCTGGTTGCAGTTGACAAATCTACATAAGTATCATTAATAGAACCTACATAATTTAAAATGCTCTCAATTGCATAAAATAAACATTTATTTCCATATTCGTTTCTTTTCAACAAAACACTTTTATCATTATCTTTAAAAGTATTAACTTTAATTGAAATATCATTATTATTTGATAATAAATAAGACATTAAATTGTATTTATTATTTTTAGTCTTTAAATCAAAATTTTTTTCGAAAGTATCTTTATTTACTGTTAAATTTTTAATATTAGCATATAGCAAAAAACTATTAAATGTCAAATCTTGATCTTTGTTTTCAATTTCACAAATATTTATTTTAAAAACTGATTTTTGCACAAAATCTACTAATTTATCTCTATTAAGTTTTTTATCTTCTTCTCCAATACGAATATAATATTTATCATAAGCTGAATAAGGTTTTTGTTCTCCTTCAGCTTCAACTTTAATTACATTTTGGTTATCAATAAATTCAATCGAAATTCTAGGCATTATTTCTGGCTTAACATTGTTTGAAATAGCTTGAGAAATATCTCTCATGGATTTATTACAAATATCTAAGTCAACAATTGTATGATCATCTTTTAGACTAAAATAAATTGTTCCCGTTCCTCTTTTATTGAGCATTGCGGTTAAAGAAATAATCGATTCTTTTTAATTCACAAGTTGATTTTTTGTATTCAACTTCTTCACTCTCTCATGGCATTTTATTCATGTTTATCTCCTTATAATTTAATTAAAAGCTATTTTTTATTTATCACTTTATTTGCTACTTTATTTATCACTCTTTTAAAAATTTGAAATAAAAATTTAAAAACTAAACTTAAAATTGATTCTTTATTACAAAAATAATTAAATATAATATTTAAAAAAATTTATAATAAAAGATATCATTAATTTAACTAAAGATAGAAGGGAGTCAAGATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTCTAAGTGCCTTTTTTGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAGTATGAATGAAGTAAGTTTACTATTTTGAAATATAACAACTAGTGGTACTAGTCAAAGCATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGGGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGAGGACTTGTTTTTACAACCACAACTAATGTTTATTTATCAATAGCTATTTACATTATTATTCCTTTTTTAGTAGGAGCAGCAGTTTTTGCAATTTTTAAATTATTTCCTTACTATCGTAAAGAAAACTGAATTTTAGATCAACTTAATGAATTATCAAAAGAAGATATTAATGCTATTGCTTTAATTAAATCATATAACCTTGAAGAACCTCGTTATGAAAAATACTTTAATAAAAATCAGGAATGATTTACTATTTATCGTAAAAATGTTGTTTTTGGTTCATTTGCTTGACCAATAATTAATGGATTTGTGCTTTTTGGCAATGTAGCTATTTTTGCCCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTTACAGCGAATTTAAGCATGATTTCAATGGGCGTTTTTAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAGTAACTAGTGGGGCTATTGAATTTAAGAATGTTAATTTTGCCTATCCTATCTATAAAAAAGGCAAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGCTAAAAAATTGGAATTATAGGTAAAACTGGAAGTGGCAAAAGTACACTTGTTAAACTTTTAACTCATGAATATTTACTAGATAAAAAAGATGGCGAAATCATTATTGACTCACACAATATTAATGAAATAGATACCAATAACTTTTATGAAAAAGTTACTCAAGTTTATCAAAAACCAATGATTATTTCTGGCTCAATTAAAGAAAATATTACTTTTTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAGAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCTTTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAAGAAAACTTAAAAGATAGCACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAAATCAAATCTTTGTTCTTGATAAAGGACAAATTGTTGCTAGCGGTAAAAACAGTCAACTATTAAACACAAGTTCTATATATAAAGATATATGACAATCTCAAGAACAAAAAGTAGGTGAATAATGCCTCGATTTAGTAATTTAAGATTTAGACAAAAAGGTGAAAAGAAAATTAGCTTTAAAATTTTCTTCAAATTTTTAAGCAATTTTCAAATTTCAAAAAGTGTTTGAATTTTTACAGTGCTTTTTGGTCTTTTAGTAGCTAGCTTAACTAATATTGCTTCTTGATTAATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATAAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAGCTTAGTGCTCGCCTTGGTCGGAATTTAAGGCTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGTTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCATTAACTAATGATATTCAAAATATAACTGAAGCAATCTGAGACATTTTAATTTCAGTTATATCAATAATATTTGAATTTAGCTTTATTACAATTATGATGTTTATTTATGTTCCACTTTTAGCAGCTATTATTTTAGCAATTGTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGATTCCATTTTAGTAAAACTATTAATTTAATTATTGTAATTGCTGCTAGTGCTGCCTTTGTTTACTACAAAGTTCCAACAGGTGGCATTAAACCTTTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGAAGAAACTATATTGATACTATTTTACAAGAAACTTATATTTTTAATGATACAGTCAAAAATAATTTAACTGTTGTTAATCCAAATATAAGCGATAAAGACTTTAATGACATAGTTGAAAAAACTTCAGTAGCCGAATTCATTAATTTAATGCCTGAAAAATTTGATACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGGCAAAAACAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAATATTGACACAATAACAGAATTAAAAGTGCAACATGCAATGAAATACTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAGTACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGACCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTAAATGATACAAAAATTGATTAGTAAAAATAGTCAGCTGCAATACTTTTGCAGCTGATTTTAATTTTTATTTAGAATATCTAAATCAAGTAAAAATTGTTTGATTCCATAAATTTTATAACCATTAGTATCAGTATCAAAAATAGGGTCATCTTTAGTAATAATAATTTTTTTAAAATTATCATTGATGTTTTTAAAAGGTCTTTTTTCTTGAATTTCTTTATTTTGATCATACATATTTAAACAAGATTGAATATAAACTTTTTGATTACCTTTTTGAGCAATAAAATCTACTTCTAATTGAACTTTCTGATTAGCATTAGGTCTTATTTCTACTATTCCAACATTAACATTGTAACCTCTTAAAAGCAATTCATTATAAATGATATTTTCCATTAAATGATTTTCTTCATTTTGTGCAAAATTGATTTTAGCATTTCTTAAACCTATATCTTCAAAATAATATTTGTATGAAGCATTAATATACTTTTTACCTTTAATATCATATTTTTTGACTTCATTAATTATGAAAGCATTTTTTAAATTTTCAATATATTTTCTAATTGTTGCATCAGAAATGTTTGTTTTCTTAATTGATTTAAAAGTTAATGATAATTTATAAGGATTAGTGTATGAACCTATATTAGAAGCTAAAATATTGATTATGTCATCTAAGTTATCAATATCTTCTATTTTATTTTTTTCAACTATATCTATTAAATAAGTTCTTTTGAACTCATTTACCAAATAATTAGTTTTAATGTTGGCTTCTTTCATGTTTTGAATTAATGGCAAACCTCCATAAATTAAATATGATTTTCATAACTCATCAAGGTTTTTAAATTCTTTGTGTGAGGCTATTTCATAGAAAGATAAGGGAAAAACATGAAGCGAAATCGATCTACCTCTAAATTCTGTAACTACATCATTAATTAAGAATTTAGAATTGGAGCCAGTGACAAAAACATTAAGTTGTTGATTATTATTAAGTTGATTTAAAACAGGCACAAAATCTTTTAAAAGTTGCACTTCATCAATTAAAAGAAAATACTTTTTTCGATCTTTAATTTTACTTTTTATAAATTCCAAAAAATTATTAGGATCACAATAAGAATGATTTTCTTGATTTTCTAAATTAATTTCAATAATATGATTTTGATCAACTTTTAACTCATTTATTAAATATTCCTTGAATAAATTAAATAACAAAAACGACTTTCCACTTCTTCTTAAGCCTGAAATTATTTTAATAAGAGGTATATCAATCATCTCTTTAAGTTTTTCTAAATATATATCCCGTTTAATTACCATAATTGCCTTTCATAATGAAACTTTGACACTTTTGTAAAAGTTTCTTCCTACATATATTTTACATTATTATGTAACAAAAAAGAAGATTATAAAATTAATTTTTTACACTTTTGTAAAAGTTTCATTATGAAAAAAATAAAAATCAAAACTAGGCTATTAACCCAGTCTCAATATATGGTGCCCGAGACAGGACTTGAACCTGCAAGCCGTTAAGCACTAGATCCTTAGTCTAGCGTGTTTGCCAATTTCACCACTCGGGCATAATAAGTAAATTTATTTTACCAAAAGATTAACTTTTTAGTTAAATAAATTTTTACTTTTTTTATATTTCTTTTAAATCTTTAAATAGCTTGGCTATTCCACTATTTTCAAAAGAAGGCCCTATATAATGTGCTACTTCTTTTACCTCATCCATTGCATTTTCCATAGCAATGAATGTTCCTATCTCAGGTAAAGCATTTAAATCATTTCCGCTATCACCAATGTGAGCAGCGTTTTTAGGATCTATTTTTAATAAGTCACAAACAAATGCTGCACCTTTACCTTTGGTTGCATTTTTATGTGTGATTTCAATTGAGTAACCTTTTGAAACTATATGAAATGCAAGTGAAGGATATTTTTTAGCTAAAAAGTTCTTAAAATTTTTAGTAGCTATTTCATCCTTACCAAAAGTTAAAATTTTAGTTACATTAGTTTTTTGACCTAAATCACTATATTTCAATTTTTCTAATTTAGCAGTTCAAGGGCGCATGTAGGCACTTGATTCATAGTTACAATAGATTTTATTTTCGCCATTAAAAATAAAAAACATTTCTTCTTCTTTTAAAAGCTTAACTATTTCATCAACTTGATTTTGTTCCATCTCATATCTAGCCAAAACTTTATTTTTCTTATCAACTATTAAAGCTCCATTTTGACAAATGGCATAAGGGCACTTTAACAATTTAACTAAGGATAAAACAAATTCGCTATTACTTCTACCTGTTGAAATAATAACTGGTTTAGTTTTATTTAATTTAACAACAGCTTTAACATTAGCACTTGAAAGAGTTTCTTCACCTTCTGGTAAGTCTAATAATGTTCCATCTAAATCTATAAAATAAGCTTGAATTTCTTTTTTCATAATTTTAATTATATATAAAGATTTTATATATGAAACAAAAAAAATATATCTATAATTTCCAAGCTTTCAGTCATTGACTAATAATGCTGTTTTATAGATATATTGGTAAAATATGTTCATTTAAAAATTAATTTTTTGCTTAATTAAACTATTTTAATTTTCTTTATTTTTAGTTTAATCTTCACCATCATAATCACTATTTTTATTTTCGACTTCTGTTATAGCAGCAGCATAGCAATAGAATCAATCTGCATTTCTATTAAATATTTTTGAAAAATCATTCATTTTTTCTAAATATATATTTTGAACGTCTTTTCAAAGATCGCACTCTTTACATTCTTCTACTCTTTTCATTAAAGAATTTGTATTAGGAACATTATATCAAAATCATCTTTCTTTTTTGGCATCTGATTTTTTAAAATGTGCGAGTTGAATTCAATATTCTGGTCCAAATTCAAAAACAATATTTAAATTAATTATAAAAGGAGTATCTTTATAATCATCAATTGTTAGATTTTCAGCTGTTAATGAAAGAGAATGATCTTTTGATATTTCGCAGCCAAGCTGAGTTAGTGATTCATCAAAATTCTTTTTTACAAATTCTCTTATCTTTTTACAGTCATATAAACTGTTGCCTTCAAAAAAAGCATAAACAATAAGGTTATAATCTATTATAATTACGCCTTTATCATTTTGAACTATTAATTCTTCGCTTCCACTGCCAAATAGTTCTTTTTGAACTCTAATATATTTTTGTTTGTTGATTCTTTTGATCAAATCTTTAATCAAAGTTGAACCAAATGATTCAATGTTTTTAATTAAAGATTTATTTTTTACAAAACTGTACAAATTTACCTCCTATACTTATTAATATTATCTAATTAAATTTTATTAAATTTCTTTTTCTTTTTTTATAACTTTATTAACTTGACAAATTGTTAAGAATTTTAAATAAGACAATAATGCAATAGTTAAACCTAAAACATAATAAGATTCAACCTTAAATGAATTAACTAAATCGCTAGTTTTAGAATCACTTATTTCTTTAGTTTTATTTAGATAAGGTAATACTTTTGTTATAGAAGTATAAATATCACTAAATAACTCAAATTTGGGATTATTGAAAATAAAACATGGAACAAATTGTTCATTTCAATACTCGTGACAATAATCTTTAATGTTGATACATTTATTTAAAGCATCTTCTTTACTATACGCTAATGATATAAAGTTTGATAAAGTACAATAAAGTGCTTTATAAAGATAAATATAAAATTCTAAAAGCTTTATAGTTTCACTGTCATTACTTGAATAAAAAGGTTGAAGATCTTTAACAAAAATTTTATTAATTTTTAAATCTTGAAATTTAGCTAAAAATTCATTATATTTTTCTTTTGCCAACTTTTGGAAACTATTATTTGAAACAGTTTCAAAATTGTAATTTTCAGTATCTTGATAATCAACATATTTATTTTTGAATACTAAAATATCCACTTTATTTAATTTGTCATTTAATGCTTTAGCAATTGAGTCTTGTAAAGACTGAATAAATTTATAAAATGAATGCTTAATTAAATAATCAATTGCTTCACTATCTTCATTGTTGTCTTTACTATTCTTATTCTTTTCATATTTGTTAGCATTATTAAATCAGATTTTAAATGCTTCTAAATTAGTAGCATTATTTAAACCTTTAGCGAAAAATAGATAATAATCTACATGTCAAATTCAAAATGAAAATTGATTATCTTCTAATCACATTAGGTTTCTAAATCATTCTTCTTTGCCTAATTCTTTTTCAGCTTCTTTTTCACTTTCATATTTAAGTCTAAAAGCATAATATTTATTATCATAACGAGGCAAGAAATAAATTCTTATTTTCTCATTAGTATTAGTAACTTTCTCTTTGATATATTTTTTTAATAATTCATAATAATATTCATCAAAAGCAGTCCAATCATCTAAACGAATTTTCTTTTCTTGATCTTGACTATAATCATATAAATATTCATTAAATTGCATTTGTGTAATTGTTATATCTAATTTCATAATCTCCTTTATTAGCTATTTAATTATATAAAAGGAGTATTCAATAATGTATATTATTGGAAATTGTGCAATAAAAACATATCCTAGATATTCTAAAATATGCTAAAAATATTATTTTGTATTTTTAAGTGCATCTTTTTTAAGACTATTGATTGTTAAGTATGAGAAGTATTCTTGATCTAAACTTCATATTCAAAAGCAGAATTATTAATACTATTTTGATTTAAATAATCTAATAAAATTAAGTTAATGCTTGCTACTAGTAATAAGTAACCATAGACTATTTCAAATTTTGCATTCTTAGAAGAAGTTATTACTTGATATTTTCTAATTAAGCTCTTATAACCTTGGCCATCAACAATGTAATAAGGGAGAAAATCGATATTAACATTTTTGAACATTTTGTAATTCAAAATATCTCAAATATTTTTATTTTTTAATTCTTTAGTGAGTTTATTGTCATTGTCATATAAATTGGTAATTATTTTTTCTTTAAAAATAAAATTAGTAAATTCATTATTCAAAGAAGTTGTTTCTCCATTTATAACAAATAATTTTAATTCTTCTTTTTGGCTTTTTAGTCTATAAACAGCAATCTTGTTTACATTAGGAAATTTAGTTGTTAAATAGTCTCTAACTTTTAGTATTTGTCTTCTTTATAGACTTTTCCGTCATTTTTACCTATATTTTCATTTATCTCTTTTATGGTTATATTTATGTAAAATATTATTTCCGAAAGTGAGGGTGGTAAGTTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTACAATATTATTAGGTATTAATGTAAAGTCAATTCCTTTCTGTTGAGATAAAAAGAGACTTTACACAAAATTCTAAACAATTTCTTTGAATTATTTTATTAAGATTGGCATTCTTCAATTATTGGCAATTCAGTAAGCCTTTTACCATTAAATTGGAAATATTTAGGTCCTTGATAAGCTCCACTACTATTAACTTTACCTTTTCTAGTATAAATTTCTCTTGTTAATCCAGACAATGATCAACTTTGATTTTCAAAATATACAGTTTTATTTGATTCAACAATTGCTTTTATGTTTGAATTTTCTATGAATGAAACAACATCTCCATCTTTTAAACCTCTACTATAAAAGTCAAATGGTTCACCTCTTTTTGTTTTTTCTTTAGTATGTACTTCGACATTATCACCCATTAATTCTAATTTAGCTTCATTTCCTAATAATTCCTTAATATCAAGTAAAATGTCATAAGCTTTTTCAGGTGGTATATTAAAAAATTCTCTATTTTTGTTTATTCTTAATTTTGAAATTCGATCAATACTTTTGTGAATCATTTTTTCAACTTCATTATATTTTTCAGTTTGTAAAGTGGCATAGATTTCATAAGGAAGTGGAACAGCTGTATTATATAATTCACGACCTCTAACTTCAGGCATTCTACTGCTTTTACCAATTTTAACCCAATCATCTCTAAAACTTGGGTTAGTTAAAACATAAACATATCCTTTTTCTGGCATTTTATCTCCTTTGTAACATTATCAATAATTTTACAACTTAAGGTGGGGTTTAAAAAGTATTTACTTCATCCAAAGAATTATAAAACTATTATATATATCTTTAAATATGAATAATCTAGTATTTGTAACTAGAAGTAAGAAGTATTTGTAAATTTAGCTTTTCATTTCAATATCAAGTTTTGTTAAAACGATAGCGCTTTAGGCTAATACCATAATCATTATTATGAGAAGATAGGCAAAAATGTTAAGGATATTACTGATGAATTACCTTTCGAAATACCTGAAAATTGAACTTGAGCAAGGCTTAAAACAGCATATGTTTTTATTGAAACTGGTGTTAAAAAATTTGATGGATTAAAAAAATATTATTCAACTGGCTCGATAACAAAAGACAATTATGTTCCTGAAGGTGAATATTCATATAATAGTAAACCTTCAAGAGCAAATAGAATACATTTTTTAGGTGAATTAATTGATGCAAAAATGCAAAAAACTTTAAAATGCGCAATTATAAATAATGAATTAAATGGTTCATTATTTTCAACTGGTTTTTATGGTTTTAAATCTATTTATAATAAAATAAAATATTTAAAACTGTTTATAGAGTCTCCTTATTATCAAATTTTAAAAGATTCTTTTTGCAGTGGAGTAACTCAAAAATCAATTAATGATGAAAAATTGCTAAATATTTTAATTGCTATTCCGCCAATAAATGAACAAGAAAAAATCATTAACAAATTAATATCATTAGATAAATTTATGAAGAAATATTTAGAAAAAGAAAATCAATTATTCAAACTAGATTCTGAAATTAAAGACAAATTACAAAAATCTATCTTACAATATGCAATTCAAGGAAAATTAGTTAAACAAGATCCTAATGATGAACCAGCATCAAAATTATTAGAAGCTATACAAATTGAAAAAAACAAACTAATTAAAGAAGGAAAAATAAAGAAAGATAAACATGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGCTCAAAAGTTATAAATATAACAAACGAAATACCTTTTGAAATACCAAAAAATTGAGTCATTGTTAAAATAAGTAACATTTCTTTTAGGATAGATAAAAAAAATATAATAATAAAAACTAAACAAATATTATCAACAGGCAAATATCCTATAATTACACAAGGCCAAAAATTTATTGAAGGATATACAAATAATGTAAATAATATTTTTAAAGTTAAAGAACCGATTATAATCTTTGGAGATCACACAAAAACAACAAAATTTGTAGATTTTAACTTCGTTCCAGGAGGCGATGGTACTGTTTTTTTGAAACCTTTAAAAATTAATCCTTTATTTTTTTACTACCTCGTGAATTATTTATCTAAAAAAATAAGAAATAGAGGATATGCTCGACACTATATTTTGTTAAAAAAAGAAATTATTCCAATTCCAAACATCAATGAACAAAATCAGATAGTTTCAAAAATTAAAAAAGTTTTTTATTTTATCAATTGTTTATAAAAAATATGGAATATATTTTAAAAGTTGTCTATAGTAGTTTGTTATGCATAGATTAACTGAAAATTTCTGCTAATTTGATAGCTCATTAATTAAAAAAATGTAGCCAAACTTCGCAACTTATTTATAAAAATAATATTTTAAATATTAACTTTTTATAAATTATATAATGTTAAGTACTATATTTATTTGTCAAAATAAACTAAAAATTATTTACATTAATGTTAATATATTTAATACTTGGTAAATTTTTTTTGAAATTTTTCTTTCATTTTTTATTGATGGAATTGGAATTAACATATTTAATAATATATTTGAATTAACATTATTAATATTTGTTCCATTAACATTTTTTCTTATTTCACTTCTATAATATTCGCTCATAAAAATTAATTTTAAATAATCAAAAATATTATTAATATTAGGTCTTATTATTCTTAAGAAAGCTCCAATATAAACATTATCAAGTTGTTCTTCGACAAATGCTGGCTTTCCTATTAAGTTCTTACTTCCTGTCGAAGCCATAATAACAATGTCATTGTAATGTAACATTTTTATGTTGTTTTTATAAAAAACAGGTAAAAAAATGTCATCATCGAATAATATTAATCTGCTGTTTTGAATATTTCCACTTCGAATTATACGAATGTAGTTTTTATCCATTTTAGTTAAAATATCGTTTTTGTCATAAGATATTCCTGAAATAATTTTAACCAAATTTAACATTCTTGTTCAAGCTCATTTTTTGGGTATTTCAAATGGTATTTCATTTGTAATATTTATAACTTTTGAACCAACCTTCTCATAATAATTTTTAATAGAATAAAAATCCCTATTCGAAATAAGGATTTTTTAATTATCAAGTGATAATTTTGTCTACAATTTCTTGTTGTTCATTTGAAGTTTTTCTTAAATAAATTCTTGTTGTTTCAATTGATTCATGACCCATCAAGTCAGCTAAAAAAGATATATCGTTATATTTTTCTAAAAAGTTTTTAGCGAATCTATGTCTAAATGAATGAGGATAAACAACTTTTGGGTTTATGCCAAATTTTAAAGCATATTTTTTTAATTGTTGAGAAATGCCTCTAGTTGTTATTGCTTTTCCGAATCTATTCAAGAAAATATATCCTGAATTTAAACATTTGTTTTCTAATCAAATGATTGCTTCTTTTTTTAATTTTAAAGGAATGTAAATTCTTCTAGCTTTTCCACCTTTGCTATAAATATCAATATACCCAAGTTTTACATGTTCGACTTTAATTTTTATAAGTTCACTCACTCTTGCACCTGTTGCAGCTAAAAATCTAACAACAAAATATCAATCGAGTGATTCTTTTTTTAGTTTTCTTTTTAAAAAAACGTAATCAGCATCACTAATTACGTTGTCAATAAAAGTTTTTTGTTGAACTTTTATAAATTTTAATTTTAAAGTATCTTTTTTTATAAATTCAAGATACCTATTTAAAGCTTGGATTTTTAAATTAACTGAATTAGGTTTATAGTTTTCTATAAGATATGTTTTGAATAACAATAAATTTTTTTTATTAATTTCTTCATATTTTAAATTAAATAGTTTAATTGCCGATTTGTAAGCATTTATAGTATTTTTAGTCAAATTTTGTTTAACTAAAAAGTCTTCAAATAATAATATAATATCACCTCCATTTTTTAATATTGCAGACTTTTTAATTATATGTTTGATTGATATTTTCGATACATAATCATTATTATGAGAAGATAGGCAAAAATATTAAAGATATTACTGAAGAATTGCCTTTTGAAATACCAGAGAATTGAGCTTGAGTTAGACATAACAACATTTTTGAAATAATTGGTGGTTCTCAACCTCCTAAGAGCAAATTTATAGAACATGAAAAACAAGGATATATTAGACTTTATCAAATACGTGATTATGGTGAAAATCCAAATCCAGTTTACATACCCTCAAAATTTGCTTTTAAACAATCAGAAAAAAATGATATTTTGTTGGCCAGATATGGAGCATCAATTGGTAAGGTATTTTTTGCAGAAAATGGCGCTTATAATGTAGCTTTAGCAAAAGTTAAAAAAATGTTTATTAATGATTGAATAAACAAAGAGTTTATGTTTATTTTTTATAAATCAAGTATTTATCAAACATTAGTTAAAAATAATTCAAGAAGTGCGCAAGCCGGATTTAATAAAGACGATTTAAAAAATCTTTTTATGCCAATTCCTTCTTTAAATGAATCGTCAAGAATTGTAAGTAAATGAAACGATTTAAATAAATTAATTAATGAATATGAAAATAAAGAAAATCAACTATTTAAACTTGATTCCAAAATTAAAGATAAATTGCAAAAATCTATTTTGCAATATGCAATTCAGGGTAAATTAGTTAAACAAGATCCCAATGACGAACCAGCTTCTAAATTATTAGAAGCTATACAAATTGAAAAAAATGAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAATAAATTGAGCTTGAACTCGTTTTAAAAATATCGCAAATTTGGTATTAGGTAAATCACCAGAAACAAATAATATTAATTATTGAAAAAATGGTGTAATAAATTGATTCACAATTGCAGATATGAAAGATAAACAAATTATTGAAGATTCTAAGAAAAAAAATATCTCTCCAAGCAAAAAAAGAAATATTTAATAATCAAATGTCCAAAAAAGGAACTTTATTATTGAGTTTTAAATTAACAATAGGTAAAACATCAATAATTAATCAAGATTCAGTTCATAATGAAGCAATTGTTTCAATAAATTTTTACAAAGATAATAATATAACAAAAATGTTTCTATTAATATTTTTAGGATTATTGATCAATAATTGTGAAAAAATTAATGCTATCAAAGGAAAAACATTAAATAAGGAAAAATTGCAAAAAATGTTAATTCCTATACCCCCTATCAAAAATCAAAATAATATTTTATTAATAACTAATAAAATTATCGATCTTTTTAAGTTTTAAAATGATTTCTTTTTGTTCTTTTATGTTTGGAATAGCCAAAAGTAAATTATTGAAAATGTCTATATTTAAATGTGGTATTGCAGCTCCTTTTTTATTTTTCTTGAATAATTCTTTATAAAATTTTAATAACATTAAAACATATTCCTGATCTATTTTGTTATTTATTTTTAATAGTTTAAATGTACTACCCATATAACCAGTTTGGTCTAGAACAAATGTTTCACCTGAATTCTCACCATCAACCAAAATAACAATATCTCCTTTATTTATTAAAACCCCATTATTAATAATTTTCGGTTTAATAATTTTTCTTAGTACCTTTGCTTCTAGATAAGGGTAATTTCCATTTTTAGAAGCTTCATTTTTTGTCAATTTTAAAATGTTTTTTTGTCTAACCCAAGCCCATTTTTTTGGTATTTCGAAAGGTATTTCATTTGTAATATTTATTACTTTCGAACCAATCTTCTCATAATAATTTTTATCTTCACCTTGAAAAATTAAAGATTCTTGTTTATCTTTCTTTATTTTTCCTTCTTTAATTAGCTTATTTTTTTCAATTTGTATAGCTTCTAAAAGCTTAGAAGCTGGTTCATCATTAGGATCTTGTTTAACCAATTTTCCCTGAATTGCATATTGCAAAATAGATTTTTGCAATTTATCTTTAATTTCGGAATCAAGTTTAAATAGTTGATTTTCTGTTTTTTTATATAACACCTAAATAGTATTCTAATAAATAGTCAAGACCTGGTATTAGCCAGGTTTTTTTGTTCAAATAGGTGAATGAGTTTACTTTTCAAAATACTTTTATTTTAGGAAAATTCTTTTATATTTAAACTTAACTTAAAAATTATTTATTATCTTACTTCATATTGTATGTGGTTTATATAAATGTATTATACATTTATATCAAACCACCACACCAACTAAGGAAATCAAATCAATAATTTTTTCCTATGCTTTAACCTATACACCGAATTTTCCTAAAGAAACAGGGAATATATAGTGATATACTTCCCAAAATTAAAATTTCAGGACTATATATACCTCCCCCTTTGAAAAAAAAGACCCACCGGAGTTTTTAATGCCCTTGTTATTTTAGAAAGGAAAAATATATGCTACTCACTACAAAACAGTTACAGGAATTTTACAAAATTGAAATGGAAAATATTGCAAAAAATAAATCTCTATTAACTAACTTCAATATCATTGATAATTATGCAAATGGTTTAAAACCTAAACAACTTTATTTTTTAGCTGCAAGACCAGGCGTTGGAAAAACTTCTTTTGCTCTTAACATTATTTTTAATGCTTTGGAAAATTTGAAAGAAGATGAATGCATTGTTTTGTTTAGTCTTGAAATGGACTCAATTGAAATATATTCAAAACTTTTAGCTCTTGCCAATTCTTGTTCACTTAAAGAAGTTGAAACAAATTCTAAGAAAATAGATTTTGAAGATTTAAACAAAAAGAACCTTTTTATATTTGATAGATATAGAAACAATTTTAATAATGAAAAATTAAGTGAAGTGACTCCTGACATTATTTATAAAAGTTTGAATTCTATTGGTTTAAAGATCAAAGCTGTTTTTATTGATTATTTTCAATTGCTTGATAATCAAAAATATTCAAGTGAAAGAGAAAAGTTGTCTCAATGCTCTAAACAATTAAAAGAATTGTCAAAAATATTTAATTGTAATTTTTTTGTTTTATCTCAACTTTCAAGAGAGTATGAAAAGAAAACCAATAATGAACCATCTTTTTCTGATTTAAAAGGAACTGGTTCTATTGAACAAGACGCTGATTTAATTATGTTTCTTTATAATTCTTCAAATAATTTTAATGTTGGCACTTTAATCCCTAACAAGAAAACAATTTTGAACTTATCAATTGCAAAAAATAGAAATGGTGAATTGGTCAAAACAAATATTGATTTTATTCCGCATTTAGCAAAATTTATAGAAAGTTAATTATAATTATGTTAAATAATAAGTGTGATTTTTGCCACAAAAGATTTCAACCAAAAAAAGAAAGATATTTTTGAGGCAATTTAGTAGTATGTTCAGAAGAATGCTTAGAACAAGTTTCTTGTTATGCAAGTGAAATTGATTTAAATTGTGAGGGAGAAAATAATGAATAAAGAAAAACTGATTAAATTTCTTATTGATTTAACAGAAAAAGAAAATACAAAATTAAAATCAGATGTTAAAATTCTAAAATCTAAACTTAAAAAAATAAAAAAAGAATATGAAGAACAAGAAAAAGCTTTTGATGACTTATTAAAAAAACTTTAGTTTATGAATAAAAATAAAAAAATAACCATATGAGCTTTGTATGATGACGGCAATATGAGTTATTTTAAAGCATTACAAAACAATGAAAATTGTGTTATTTATTCTATAGGAATACAAGAACATAAAGATTTAAAGAATTATTATAATATTGACCTTTCATTAAATAATTTTAATTTAATTGACCAACTAAAACTAATTCCAAAACCTGACATTATAATTGCTTCTCCACCTTGTGAGAGTTGAAGTAGAGCAGACTGTTCACTAGCTATTTGAAAAGATATTAGCAAAACAAGTTGAGAATTGAATAACTATGAATTTTATAAAACAGTAGAAACCACAAAAAACAAAAAAAGAGATTTTTACTCTAAAGAACAAAAAAGAGTATTAGGTGAAAGTACTGCTGGAGCGACGGCTTATATAATTAATGTCTTTCAACCTAAAGTGTGAATAATTGAGAATCCTTTTTAGTCTTTTATTTGAAAATTTCTGAAATTTTACCATAACTTGAATGGAATTAAAAATAATACCTATTACTCAAGCTATAACAAAGAATTTAGTGTTAAGCCAACTTGTTTTTATTCAAATATTAATTTAAATTTGAAAAACAAAAGAAAACCAGGCAATGCTGAACACTATTCAAAAGGTAGTTATTATCAACGCTCAATGATTCCAAAAGAATTAATTAAAGATTTATTTTTAAATGTTTTTGTCCAATTGAAAAAAATTTCTGTATTAAGTAAAGAATATACAAAAAACTATATTTCAAATTCACTAGAGAAAGATCAGATCTCCTTATTTGATTAAAAGGGGATTATAGGGGTTATTTATATAAGTATAAAAATACTTATATTAGAGAGATATATATGAGCTACACTCATTATATATATCTCTCTCTAAGAATATAAGAATGCATTACATACTTAGAAAGGAAAAGAATGGATATTTTTAAAAAAATGGAAATTGCTGAAAAAGATAAAATTGAGAAATTATTACTCAACATAGATAAATTCATTTGAGATTGTAATTCCAAAGGTTGAACTAAATCAAAAAACAAAAAAGTAATTTCTTTATACAAAAAAACTCTTGAAGAAATTAAAAAAAATGAAAAATATGATTGGCTATATGTTCCTATTAAATCACAAATCTATGAATTTGCAGATCATAATTTAAAGTCTGACATATTTTTGTGATTTAGTCACACCAAAATTTTATGTGGCTATACATATTTGAAAGATAAATGAAATTTCTAAAAGAACCTCCTGTTTTTACAGAAGAACAATTTAAAGAATTAGTTCATTATACTGATTTAGAAGATAATCCAACTTTTGAAGCTTACAAATCTAATGCATTTTTCAAAATCTATTCAGATGCTAAAGGTGTTTCAAAATCTTTTAGTAGAATGATTGAAACTGTTTATAGATTAGTGAATGAAAAAAATTTTAATAGTTTTTGGTGTAGAAATCAATATAACCACATTAAAGGAACATTAAAACCATTATTAGAAAAAGTTTTAAGTTTTCTTGCTGCTAAAGGTTTAGATTATAGACCTTATATTAGTATTTACAATACTGAAGCTTATTGAGATTATGAAGATGGTGGAAAAGGAAGAGCTATATTTTTTGGTAATTGAAAAAATGTTCAAAGTTTCCAAGGTGTAACTCTTTCACAAAAAGATTTTGCATGGGGTGAATTAGTAATAGATGAACCAATTGAAAAAGAGCTAACTGAAGAATTAGAAGAAATTTATAAAATTCAAGCAAGTAATTTAGAAATATTAATAGCTAATACTGTTTTAAGATCTAAAAATATTGAAGGGTTTAAAACTAATGTAACTTTTCTTTATAACATTTTCACAGTAGATCACTTTTTAATTAAAGATTTTCATAATCCTATTTTACCTTTGTATTCAGGAACAGATTTTTTAAAGAAGATCAATTTAAATTTAGCAAAAGAATTAATTGAAAATACTTATTTGCAAAAAGAAGACTTAGCTTTTAAAGAGGGAATGGGAATTATTGTCACAATGTTTTCTAAATATTTTGTTCCTAAAAATGAATTAAGTGATTTGCAATTAAAACAATTTGAAGCTCTAAAAACTAAAGATTATAAATTATGAATGATTACTGTTGCTGGTTTTGCTTTTGAAGAGCCACCTGAAAGATATGATTATTTTATGAAAAGTGTTTTGCTCAACAAAAATGGCAATTGAGATAGAAAAAAATGCAAAATAATCAATATTAAACAATTAGAAACAAAATTAGAAAATGAAGAAATTTGTGGACTCTTTTATGGTTTTGATTATGGTTTAACTGATAATTGTGCTCTTGTTGTTGTTTTGTTGTTAAGTGGAGCAAGAATAATAGTATTAGACATTTTTGAAGACATTAAAAAGCTCTTACCAAAAAACAAGAGAAGAGAAAATAAAGCTATTTACAATAAAGTTGCTTTAATTGTCAAAAAATGAAATGAGTATTTTGATAAATACAATTTTAAATTCTATAACTCATGATTATTAGGCAATTTTGATTCTTTCTTATATGGAGATAATGTCCACTCTTTGCAATGAATGACTGAAGTTTTTATTAACAATGGGATAAATACTAAATTGTTACCTGCTTCAAGATTTAAAGGCAAAAAAGGCTTTGGAATAATTGACAGACAAGTATGACAAAAAAACATATTTGAAAATGGTTTAGTTGAGTTATTACCTAAAGCTAAATCATTATTAACTCTGTTATGTCAACAAGTAATTGATAAAGAAGATCCTAAAAATCCTAACCAAAGAAATGAAAGAATTAACAAAAAAATTTATGATGTCATCAATGCTTTTGAAATGGCCAACACCTTACAAAATTGTGAATATAAAAACTATTTATTTAATAAAGAATTAAAGGAGAGAAATGAGCAAAACAATTATTAATAAATTAGAAAATGAAAGCAACAAATATTTTAGTTTAAGGTTTAATAATAAACCTGATTCATATAATCAATTTTTAAAATTTGGAACTCAAACAACTATTAACACTATTTGAAGCAATTTACCAAAAATTTTTAAGAATAATGTAGAAGTTAAAGACTATTTTACAATTTCAAAATTGCAAGCTTTGCAAAAATTAGAGCAATATCTTTATTGTGAGGGAGTTGCTGCTCTAAGAAATAATTGAATGCCTGCTCAAGTTTTAGATTATTTGCTTGATAGAAATGGTAACTTGGTAAAACTTAAAACAAGAATCAAATTTTATTCATCAAATATAAGTTTCTATATTGATGAAGAGTGAATAAGAATTAAAAATAAAGTTGAATTTAAATATGATTTACTTTTTGATCCTATAAAAAATGTTCTATTAAATGTTGAACAAGAAAAACAAGCTAAAGCTTTATTAGATTTTTATGACTATGAAACTCTTCCATTTTATAACCAAATTCCTTATCAAATTTTCTTTAATAATCATAATGCTTTCCCCGATTTGTTTAATGTTAATTTATATTATTTTGATATGCTAGATGAAGACTTAAAAATTGCTGCAAATGATTCAAACTTATCTGCTCCTTGAATTTATGTCAATGATATGAATGGTCTTAAAGACATGATCAAACAAGGACTTAAAGATCCTAATGATAGATTTATTCCAATTAATACTGCTATTCAAATGGATGGAACTCCTCCTGTTCAACTTGTGCAAGGCAATAGTCAAGCAATACCATTAATTGAAAAAATAAGATTCAATATTAAACAAATTAAAAAATTTGCTTTCTTAAAAGAAGATTCAAGTGATTCTGGAACTAAAAATATGCATAATGCTGAAGTTCAACAAATTAATAGTGATTTTGAAGATCATATTGAAGCAAAATCAAACTTAAGACAAATGCAACTATTAAACTTTTTCAAAACTTTTGAACCTAGTTTGCTAATTGATAAAGTTTTAATTTTTGGTTCAACTGAATGGCTAAAAAATGAAGCTTGAAAATATTCTGCAAATTTAAATGGCTCAATTATAAATCAAACACCTTTAATCAATAATAACTCAAAAGCTTTAGAAGAAAGTGAGGAATTATAAAATGGACTTTATGGAATTAAAATCTTTTTTAGAGCCAAGAACTGGTGAATATATTTTAATAGACAATAAATCACTTTCTTTTGAAAATGGTTCAGTTAATGTAATATCTTGAACTAAAGACAAAATCAAAAACATTGAATTATATTGTTCTGTTGAATCTCAAAATAGAATTGATATTGAAAAAGATATTTATGATGTCATAAAGTTAAGCATTGATTTCGAACAACCTATTTTTAAATATAATCAGAGTGCAATTTTGAAACTAGATAATAAATTTTATATTTTAGATGCTTTAAATAACCAATATGATTTGGAGCAAAAAAATAATGCTTATTCATATGGAATAGCTCAACCAATTTCTCAAATTGAAAATGAGAAAAAAATAAAATTTGTTTTCATTGAAGAAAAGCCAAATGAAATTAATGTCAGTTTAAGAACTCCATATTTGCACCAAAAAGCTTTAGAAATTGAAAACAATGGTTTAACTAGTGAATTGCCAACAAATGGCCTAGCTTTTGATATGTTTGTTAATCCAATTGAAATTAATGGTATTGACAATAAATACTATTTAAATACTTTGAGCAAATTTTCTCATTTATATTTGAATATTATTTTGAACAAAGAAAATGATTTAATGAAAAACTTTTGAGGTGCTATTAATCAAATTGATAATGTTAGACTTAGATTACAAAATAATTTCATGGGAATGACTGAAGAAGAAATAAAAAAATTTAAAGAGACTATTTTTGCTGGAAACAATTTAAAAGATTTTGTTGATTTTAAATTTTACACTTTGATTAACTTATATACAAAGAAAGAAAAATTTTTAAAAACTTTATTTGCAATGAGTTCAGGCAATGTTCCTTCAACATATTGTATTGATAATGGTGAAACTGAAAAACATGAACTTTATTTGCCTTTTTATTTTGATAAAAATTGAGAAATAGCAAGTAATTTTTATGATTTAGTTTATGAAAATAATTTGATAAAAAGCATAAAACCAAAAGCAATAAAAAGTCCAAATAAGCTTTATCAAATATCTGAACCACTAATTCCATTAAATATTCAAGAAGTCAATATTTCTGATAATTGAAAAGACTTTGATTTTAATAATTCAAAATATAAAGTCAAAATGTTTATTGACCAAAAACATGAAGAATTTTTAATGTTTAATAAAGAAAAATTTAATGAAGCTTTTCAATTATCATCAATTCAAACTTCTAGATATACTATGACACCAGAAGAAGCTAGAAAAGAAATTAAAGACTTAGAACCTGGTTTAACTAAAAAGCAAATGTTTGTTCAATTAATTAAAAAAAGCGTTAAAAAAGAAATAAACATTGTTAACGCTTTTGCAAAGGTTCAATTAAACCCAACATATACAGCTAAAATTGCACATGGTGATGAACCTGGAATGATTCATTATACTTATGACACAATTCCTGCTGCATTTAAAATTGTTGATTTAGATGATTTAAATGATTGGTTAGAAGTTGTTAATTTTTCAGGTCTTGATTACAAAAATCTTGACGATAACTATGAATTAATAAATGAAACTTATGTTCCTATTTATACAATTGAATATCAAGTATATGAAAAAGAAGATTTTAAATCAAATATATATGACACTTATTTTAAGTTTCTAAACAATGATTTTATTATCAATAAAAATCAATGATTAAAAATTGCAAATTCTTTAGGTTTCGTTCCACAAAACTTTATATGCAAACAAAAGAAAAGAATAATTATTTTTGGTTTATTTTTACCTAATGAAATTTATATTAATAACCAAAAAATAGAATTACCTTATTATCACAATTTAGGTAACTTAACAATTAACTATATTTAATTTAAGGGAACTGCTGCCCAAAAGACGGAAGAAAGGAAGCTATGCATAAAATTATTAGTGTTTTATCTAGCAAACTAGGAATTGAAGAAAGTGAACTCCTTAAAAAACTTTCATTAAATGAAAATTCTTCTCAAAAGGAGATTGCTTCTTCGCTTGGAGTTTATGAGTTTTTCCAAGAAAAAGATGACTTGACTTCATTCATCTTAAATAAAACTCAAAACCAACATAAAGATAATCAAAATTTAAAAACTGAAAATGAAAAGTTAAAAAATGATTATCAAAGTCTTGAGCAACAATTCAACACCAATTTAGAAAATTATAAAAACTTGTCCGGAAAAGTTAAAGATACTTTTAAAAATGAATGAAACAAATACAAATTGAATGAAATTGATTTTGAGAAGATAGATGTAAATTCAATTAATCTAAATAATTTGTTTCAAAGTGTTGAGCAAGTGGCAAAAGAAAATAACATAACTATTAAGACTAAAGAACCTAATTCTGTAAATAATATTAACCAAGATTCTAGTTCTAATAGTTCTGATCCATGAAGTCCTTATATTAGAAATTATTAGAAAGGAAATTATATGTCTTATCAAATACCAACTTTAAAACAATATTTAACTGCTGAATCTGCTACTTTATTTGAACAAGGAGCTGATAAAGCTTATACCATATCAAATGGTGGTCTAACTAAAATTTCAACTTTATTGAATGCAATTTTAGAAACAAACTTTTTAGGTAGTACATTAACTCCTGCAAGTGATCCAAATTCACCAGTTATTGAATATTTAATTTCTTTAGATCAAGAATGAAAAACTAAAGAAGAATATACAAAAAACCCAAGAGCTCAAAAAGCAAGATATGCTAAAAAAGAAATCCGTTGAAATGAATCTCATGTTCAAGATATTCAATTAAATAAAGTTGATTTATTAATGAATGTTCCTGACATGCTTGTTCAAAAAGCAAGTGACGAAATTTCAAAATGAAAGAGAAAAGTTGACAAACAAAATTGACTAAAAGTTGAAGAACACATTAAAAACTATGCAACAAAAAAACAAAATGGACAACCTGATTCAATTGTCACTGAAATTGATTTGACTAAAAAAACAGATGAAGAAGTTTATAGTGAAATTGTAAACATGGCATTAAGATTAATGGAACATGAAGATTCAACATTAGGAATTGACGGAATTGAACAAAGCAAAATTGGAATTTTTGTCAAACCAAGTATTTATCAAAAAATAGTCAGATATTCAACACAAGGAAACTTTGTAAAAGAATCAATTCAAGGTAATCCTAAAGACCCAAGAACATGATTATTAAGTGATTTCCAAATGATTGCAGGAATTAAAGTCAAACCTTCAACATTATTAAAAGAATTTGAGGCTGTTGCTTGAACAAGTGATTCTGCTGGTTCTGGAAGCAGATTAATTTCTCTTAAAACTGACGAAGTTCCAAAATTTAGTGATAGATTTGTGGCTATGGAAATTCAAAACTTTTTTGGAATAGTTTGTGATTCAAACTTTGAAGCTATTAAAAAACCAACAACTGCACCAAGTCCAAAACCTGGCTCTTAATAATGTTAATTTATTCTAACCCAAAACCAAAAACAACCTTGCAATTAAGCCCAAAATCATTTGTTATTAATGATTCAAAAAAACAAATGAATCCTTTTTTGCGAGGTTTTCTTTCATTCCTACATGTTGCTTTGGATATAGGAGCAAGTTTTTTAGGTAGTTTTATTAATACCTTTGCGCCTGGTCTTGGTCTTGTTATTGAATATGGTCTAAGTACTGCCTTAGATTTAGGTTTTAATGCTGCTATAAACTATGGAAATATCAATTGACAAGACTTTGGAATTAGCGCTGGAATTAATGCTATTCCTTTTGTGACAAGAGGAATAAGATATGCAGCAAACACACATAAAACTAACAAGTTTTTTCAAATTGCAAAAAATCTTAAAAAAGAGGGTCAAGAAGAATTAAGTGAAAAAATAACAAAAATAGCTGCTAATTATTCTGAATTAGGAGTTGACAAAGCTAAATTTATTGACAATAAATCACTTTTAAAAAAAGAACTAAAATTATTTAATAATGATTCATTTTTGAGAACTTTATCTGATAACAAAAATATTTATTATTTTTTGAATAAAAAGGTTCAAAATATACAAAAAGCTTTCACACATTTAAGATCTATGGTCTCATTATTAACCTCTCCCTCATATTTAGCAAAAAAAGCAGTTGACACTGTATTTAAACACCCAAAAGCCTATTTGAACAAACAACTTGGCAAATTTACCAAATTATTAAAAAATACTTTTAAATCAAAACCAATTAAGAAAGTAGTTAAGAAAGCTTCTAATTATATTTGAATTGATCATAGTTCTTGATTAGAAAGAATGTCAATAAATAAAGTGAACAACCCTTGAGATATTAAAGCTATAAATGCTGTTCTCTATTTTAAGAAAAATGCAACAAAAAGCAAAAAAAATCCTAAAGGAAAAGAACCTGTTAGATTATGAAATAAGAGCATAGATGAATTGATTAAATTAGTTTGTGCTAAAAGTCCTGGAAGATACTATTTAAATAATATTGCTTATGGCCATATAGTAGGAAGATTTATTAGAAAATTTGAAATGTTTCAAGCTGTTCCAATACACCCTATTTTCTCCAATTTAATAGGAACTTCATTATTTACTTTTAGAACAATTAATTCTCTTATTATGAATTTTAAAAGACAAAAATGAGTGTGACTAGAAGACAAAAACATAATCTTAAATGAAATTGTTAAAGGAGTTAAAGAAAATGTTTTTAAAGGTTGAAGAGCACCAGGATTTCAATTTATTAGCTCTATGGCTAGATCATGAGCAACTGGCAATGTAGGTTATCAAACAAAAGCCGCCTTAAGGTGAGCAAATAAAAAAGCTTTTACTAGTAAAACTCAAAAGACTAATTATTGAGTTAATCACTGAGTAAAGAATAAATATAAAAAATAGGAAAGGAGTTAAATTAATGGAAGAATTAAACAAATTAGAATTAAATTTGAATGAATTTAAATCAAAATATTGATATGAACAAAATTTAACTATTCCTTATGTTTTTAAAAAACAAAATGAAAATTTAAATGAAGTAGATTTTTTTAATAAATTTGCTTATTCAATATATACAACAATAGATTTAATCATAGGTCAAAAAATGAGTTATTTTGTTAAAACTCAAAGTGACTCCAAAGCTATTAAAAATATTATTGGAGAAACAAGATTTGAAAACTTCAAAAAAGCTATTTATTTGGAAATTAAATATAGACTTTCAAGTAAAAGTTTTCCAGAATATTCAAATAGTGTTTTTCTGGTTTCTAATAATATTTCACTTAATGGTGAAACAAGAGATTTTGGTTCATTATCAAAATTAATAAATTATGAATCTATTCAATTATTAACATTTCCAAGTTGAGTTGATTTAGAAAGTGACATTGATTTTATTCAAGCTGATTTTGAAGCTTTATTGAAATCATTAAAAGAATTTGTTAACAATAAAGATATTGAATTAGAAAACAAAATAAAAAGCTTGGAAGAATATGACAAATCTCAAACTCAAAATAATATTAATCAAGAAGCGCGTATTAAAAATTTAGAATATAAAACTAATGTTATTAAAAGCAAAAGCGAGATACGACTTGAAGACAATGAGGGAAAATTTCATTTATGAGGTAACGGTTTCGGATACAAAAATAATAACGATGTGCTTTATGCATTTCAAAATGAAAGTTTTAAAAAACTAAAAGACATTTTAGACAACTACGAAAATTTAAAGCAAGCTTTTGAATCCTTTAAAATTGCGCAAGAAAACCAAAATACTAAAAATGTCCAAATCAATTCCACACAAGATAATCAAATAAAAGCTTTAGAAGATAAAGTTGCTACTTTAGAAAATACCACATCTAATGTTTGAAAAATAGCTGAAATAGATAAAAATTTAATTTCTTTATATAATCAAATAAATAGAGAAAAAGACAAAAAATGAATAAGAGTCGGTTTAGTTGGAGAAAGAGAATTTGAATATGGCAAAGAGCACACAATTTCATTAGATAAAAAAAGGACTAATCTAAATTATGAATTTGACCTAAAAAAATTTAAAATAGTAACTTATATTAATTATGCAGGGAAAGAGAATAACTTTATTACATATTTAAATTCTGGTGATGAATTAACTTTTTGTCAGCCTGTGGTTTATGATGATATCGGCTCAAGTAGTGCATTAGCAATATGAAATATTAAAATATTAAATGAGTCTTGTGAATTTAAAATTATACAAGTTGGCAAAATTTTTAATAATGAAACACCTAGTGATTTGCGTTTTTGAAAAATAGACTTATTTAGCTGAGAAAGAACATATTAATGATTAAAAGATTTTTCTTATGACTATTAAGCATTTTGCTTATATTATTAATTATTTCAATATGTTTTTTAGTCTTCACCATTGTTAAACCTGAAAGCGCAATAAGTCAAGAATTAGCTTCATGATTAAAACACCTATTTAGAATAAAAATTGGAGGTTAGAATAAAACTCTATATTTTAAAAATGAAAATTGAAAAAAGAAATTTAGCTAAAAGAATCAAAGAAGCTTCCAAAGCTTTGAAAACAAATACATTAGACAATAAAATTGAACATGATAAGAAAGGAAACAAATAATGAAAGATTTTTTCGCAAACCCAACTAACTTAATTATATTTTTAGGTGGTTTTGTTTCTTTAATAGCTTCTATTACTGGAATCACAACCACTTTCATTAAAACTCATAATTTAAAGCTTCAAAATAATAACCTTAAACAAGAAAATAAAGACATAAGATTTGAACTTCTTCAAACTCAAAAAGAATTGGCTGATAAAAACAATTCACTTTCAAAACTTGAAGTTATGTTTGACAACCAAAGAAAAGAAATTGAAAGACTAAGTGAAGAATTAAAAGCTTTACAAAGTGGCAATGTTGATCCTTTGGTTAAAAAGCTAAAAGAACAAATTGAGAACCTTGTTAATGAAAAGAAACAATTAACTGAACAAGTCTATAATGTTAAAGCTGAATTAAATGAGTCTAAATATCATTCTGGAGCTTATGCAAATGAAATTGTTGAATTGAAAAGGGAAAATAGTGAGCTTAAAGAGCAATTAAAGCTTGAAAGAGCTAAGATTGTTGAAGACATAGCAAATATATTAACTCCCAAAAACAATGAGGCAAATAAGGCTTCTAATGAAGCAATAAAGGAAAATAAAATAGAAGCCCCAAAAGCAGAAACCTTTGAAGCTCCAAAAAATAATGAAAATGTTAGCCTATATATTTAAAAAACCTTTATAATATAATTGTGAGTAACATAGCAAATGGCAGTCGCCATTTGTTTTTATTACTTAATATTTTTAATTAAATTTTTTAATTTACTTATATTGCTATTTAATACTTCTGTAAAATTAACATTTTCTTTTGTTTCACCTAAAACTTTTTTGTATTTGTCTAATTGGTTGTTTAGTTCACTAATTGTTAATTTGAGTTGAGCATTTTCTTCGCTTAGTTCTTTATATTTAATTTTAATTTTTGAATAGTCAAAAGCACTTTCATTTTCCATTTGAGCTTGTTCCATAATATTTTCAAGAGCAACATTGCTAAAGTTTAAATATGTTGTTGCTGTTGTTTGTGAGTTAACATGACCAACAAGCTTTGCAACTTCTTCTGTTCTTCCAATGTTTAAATGAGCATTAACAATAAAGAAGTGTCTTAACATGTGAGCAGAGATTTTTATGCCTCATTCTGGCTCTAATTCATGAACATAATGTTTAAATAAATTGAATTTATTTTGAATGGTTTTAATGTTTAAACCTTTTTTAAAGAAATCAATATTGTCAATAATGTCTTGTCTTAAGTTTGCAAAATAATGATTTGTTTCAGGTTTTGGAATTGTGAAAAGTCTAGTGTTATTTTTCTTTTCTGTTTCAATTCTTTGAGTGAAATTATTTTTAACTAATAAAGTTCAATTAACATTTGCAAGTTCTCCAATTCTAACTCCTGTAATAGCTAAAATTTGGAATACTAATTTGAATTTAAGATCTCCCCATAATTCAACATAATTGAATATTTTTAAATATTCTTCCATACTATATGCTTGTCTAGTTCCACCAACTTTTTTAAATGAATCTAAGTCTAGTTTAGTATTGATATAAACTCCTGTATATTCTGATAATCATTTGATAAAAGTAGCATAAACTAATCTTGTACTATATTTAGTGTTTTCTGCTCAATTTGAAGAAGCTAAATAATGATTCATTTGGTCTAGTAATTCTTCAACTGATCCAAATCTTTTAAGTCTTTTCAATAAAGTTAAATAATGTTGAAAAGTTTTTTTACTTTTTTGTTTTCTTTCAACTAAGATTTTAAATTGATCTAAGTAAACTTTAACATAGTCAATTTCTTTATTTAAAACAATTTTATTTTTGTAAAACATGATTACTCCTTTTTTCTAAAACAACTATAAAACAGTTATTTTAGTTAATTTTATTTATGTATTTTTATTCAATTAATTAATCTTTGACAACATTTTTGACAAAAATAAGGAAACACATATTCTTCATTTTTATAATTAAAAACTTCTCTCTTTTTGTCATTTGTCAAAATTTGACAACAACGGCAACGCCATCTGTTCATTTTTATTTTTTCAAACATAATAGTTCCTTTTCAAATTTAATCATTTGCCTTTTCCCTTTCTTTCAATTTGCCATCAATTGCGGTATATACCATATTTAAATAATCTTCAAATACTTGCACTTGTACTGGAGTTCTTAAATTGTCAAATTTTCATTCGTTAGCTTTGTAAGACTTAACACATAGCTTAATGTTTTTTAATCTTATTTTCATTTCTCTTACTATGAAATATAATAGGAAATTTGATTTAAATAATTCCATCATATTGCTAATTCAAGTAAGATAGTATTTTATTTTTCTTCTTATTTCTTTATATCAAAACTCTATAAATTGTATATGAGTCATTAAATGACTGCAGCCATGTTTAGAAGAATTGACACAACTCATTAATTTGTTAATATCATAACTTAATGGTAAATCTAACATATCACTAACCAAAGCTCACAAATCATTTAAATCTTTGTCACTACAATTTTTAAATGTTTCTCTTGAAATTAACATTTTTATTTTCCTTTCTTTATTTTATAAATCTGGATACTTTCTAAATTTTTCATAAATTTTATCTTGTGTTTCCTTTAAACTTTTTAATTCTGCTTCTAATTCTTGTATTTTCTTTTCTTTTTGAGCTTTTCTAGCTCCTCTTAAATTAGAAAGAGAATTTATATTTTTTCATAATCTTTCTTCAACAATATCTATTCTTTGTTCTGTTTGTTTTAGAAATTTCATACCATTTCTTTCAAAATATTCTTTTTCACTTATTAACATTTTTATTTTCCTTTCTTTCTTTTTTAATATATAATAAATTTGCGGTTGTTTGGTGTTCAATTAAAGGCAAATAACCGCCTTTTTATTTTGTTTAAACCAAACAGTAATGATAAGTAAAAACAAAAGTGAGCTTAAAACCTGCTCACTTTTTATTATTAATATAGAAATAATAAATATAGTGATTTGTAAAAAGTGTTCAGGTTTTTACTTATCACAATTATTATAGCGGGAATATATAAGAATGTCAAATAAATTTATGAAAATTTTTCACGTTATAATTTAACTATGAAAATGCCAAACTATAAAGATCTTAATTTTAATATCAAGAGAGAATTAAATAAATTAAAACCTAAAAGCTTTGAAGAATGAAAAGAAGCTAAATCAAAAGTTTTTTGCCTTGCCCTTGGTAAATATTTAACCAAAATTAATAGCCACCAACCTGATATATTTAAAAATTATTATCAAGTTCTTTTTTATCATTCATTTATTAATTATAAAAGAGAAGAAGAATTATTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGAAAAGAGTCAACTGAGTGAAAATTCAAAGAAATTTAACATTTTTTAACATATTTAAAATTTTGATATAAAATAAAGTTGAAAAGTGAATTGTTTTATTCACTAAAAATCTTAAATACCAGATATTGACGGGAATATATAGTGGGAATATATAAAGGAATATATTTTTATATAATGTCAATTGTGTTAACAATTTGTTATATTTAACTATAATTTTTAATTGTTCTTCAATAGAGGGAACTGGAATAAAAAACAATTTTATGAGCTCTATTGAAATATTATCATTTGTAGAATTTTTATTTTTTCTGATAATTTTTGATATTAGTTCACTTTTCATAACAATATCTACATAATCAGGTAACATTTCATTTAATATTTTTATATTGCCAACTCTTTGATTTACAAGAATTTTTTCTTTTAATTCTTTAATTAATAAAGATTTACCAACTGTTCCACCTGTCATACAAATAACAATGTTTTTATTATTTAAAAAATAATCAAACATTTTACTTTCATATTTATAATAAACAATATTGTTATTTTTTAAACCACGTTCATCAAAATCAGATATTCTTAATATTCTTATACCATTTTCCTTTGACACAAATTCTGAACTTTTAAAAGCAAATCCTCCATTGATAATTGAAATATTTTTTAATCTTACTCACATTCAATTCTCTGGTATTTCAAAAGGCAATTCTTCAGTAATATCTTTAATATTTTTGCCTATCTTCTCATAATAATGATTATCCTCACCTTTAAAAATGAAAGATTCTTGTTTATCTTTCTTTATTTTTCCTTCTTTAATTAATTTTTGTTTTTCTTGATAAATTCTCTCAATAAGTTTGCTAGCAGGCTCATCATTTGGATCTTGTTTAACTAATTTTCCTTGAATAGCCAATTGAAGAATAGATTTTCTCAATTGTTCTGCTGTAAGTCTATTAATCTTCATTTAAAATCCTTGTTATTTCTGCTAGTATTTTATCTATTTCATCATTTAAAGTTTTTCTTTTTTCTCGATATTGAGAGATTAGTTCCATTGGTTCTAATATTTCTTCTTCTTCATGAGGAAATTGGCATAAATCTAAATCATAATTTCTATTAATAATTTCTTGTTTTGAATATTTTTTAGCCTTTGGGAATTCATCAATTATTATTTCTTCTCTATTATCTCATCATTTAATGACTTGATCAAAATGTTCTAATTGTATTGGTTTCGTTTTAGAAAAGTTTTTATATCCTTCTGGCATATCCATTCTATAGAATCAAACTTCTTCAGTTGGATGAGTGTGATCAAAAAACAAAATATTTGTTGTTATTGAAGTATATGGAGCAAAAACACTATGCGGCATTCTAATAATGGTGTGTAAATTAAATTCTTTTAATAATTTAGTTTTTATTGCAACTTTTGCATTATCTGCTCCAAATAAAAAACCATCAGGCAATATAACTGCACAACGTCCTTTTTTCTTTAAACGATACATAATAACGTTCATAAATAAATCTGCAGTTTCACTACTTCTTAAATCAGCTGGAAAATTATTTTTTACAGCTTCTTTTTCTGAACCACCATATGGGGGATTCATTAAGATAATGTCGAATTTATCAGCTTCAGTATAATCTTGTACTCTTTTTTCTAAAGAATTATCATGAAATATTTTTGGTTCATCTACATCATGAATTAACATATTAGTAATACATAAAAGATATGGCAATGGTTTTTTCTCAATGCCATAGACTGAATTATCATATAAGTTTTCATCTTCAACTGTTTTCTTTTGATCCTCTAAATGCTTCAATGAAGATGTTAAGAATCCACCTGTTCCGCAAGCAAAATCTGCAATTTTTTCTCCTAATTTTGGATTAATCATTTTAACCATAAAATCAGTTACTGCTCTTGGTGTATAAAATTCACCTGCATTTCCAGCACTTTGCAAGCTTTTTAAAATAGTTTCATATATAGTACCAAACTCATGTTTTTCTTTATAGTCAGCAAAATCTAATTCATTAATTACATTAATAACTTGTCTTAATAAAACACCGTCCTTCATATAATTTTGTGCATCTTCAAAGACAAATCTTGCAATTTTTTGTTTTAAAATAGTATGTTCATTAATTCCATAAAAAGCTTGTTCGCCATTGCTCAGTGTAACAGTTTCTCCTTTTAAAAAAGGAAAAAGTTTATTATTAACTAAATCTAATAGCTTTTCGCCTGTAATTACATTGTTGCTATGATCATCAGTTGCTCATGTGCTTCATTTTAGTTCATTTGGTATTAAGCTTTTATAATCTTTATCTATGAATTCTCATTCTCTTTCTTTTGCATCATATACTTTAAGAAAAAACATTCAAGTCATTTGACTAATACGTTGAGCATCGCCATCAACACCTGCATCGTTTCTCATAATATCTTGAATTCTTTTAATTAAATTTCCAATAGCCATTTTGCCAATTCTCCTTTTTTATACTATATAAAGTGCTTTTTGAATTTCTTTAACAGTCTGAATATATTGTTCTTTTCCACCAAAAAGTTTAGCAATTTTTAAAGGACCGCCAAATTGTTCAAATTCTTTTAATCTCAAATTATTGTTATCTGTTAATTCATCAATGACTTCTTCTTGATATTTATTTATAAGAATTTGTAAAATTTTTCTACATGTTTCAACATTTTGTCTTTCTATTATTTGAACAACTTTTTTATTATTTACTCTTTCAGATCTTTTTAGTAATTTTGATCCATATGCAATGCTACAGATTAAATCGAAATCATCAAAATCTCTTGGATAAATATTTCTTAAGTTTTCAATTAAAATGCCTTTGCTCATTAATTCTTGAATTATGGTAGATCTTGTTTTATTACTAGTTCAATATTTAACAAAATCTTGAATAGTGTTATAGTTTTTTAAAACATTTCTTTTTACAAAATCTTCAATAGGTTCAATAATTAATTTTCCTTCATCATCATAATAGTTTACTTTTTTGTAGATTAATTCAACATCTACACCATTAACATATATTTTCTTTCTTCTATTGCTTTTATTTTGTTCTGAGTTTTCTTTTCAGACTAATCCCTCATCAACTAATTCATATTCTTTTTTTGTTTCTTGTTTTGCTTTTTTATTTCTAAGAATTTCAGGATTAAAAAATTCTTCTTGGATTGCTGGGCCATTAAAATCAGGATCAGCAAATAATTTAGTAGCATTTTTAAAGTCTAAAATAGTGAAATACAATTTGTTTTTATGTTTATCTTTAGGATCATCAAATTCTTTAATTCTGGTGCCTCGGCCAATTATTTGTTTAAATTTAGTCATACCATTTTCACCAAAAGTATTATCAAGTGCAATAACACGACACAATTTACAATTGACACCTGTAGTCATAAGTTCACTGGTTGTAACTATTGTTGGATATAGCTCATTATCATCAATGAAATTATCTAATTGTTTTTTACCTTCTTCATTGTCACCAGTAATACGAACAATATATCTATGATCTTTGGCCATCATATCACTATTGTAGTGAACTAAAGCTTGTCTCATTCTCTCAGCATGTTCTATGTCGTCACAAAATACAATTGTTTTTGCATATCTATTGTTAATTGATTTTAAATAATCGGTAATTTTTTTAGCTACCTCCTTTGTTCTTTCTTCTATTACAATAGTTTTATCAAAATCCTTTACATTGTATTCATTGTCTTCCACTATTTCACCATATTTATCTCTTTTATCTTTTTCTGGTCTATAGCCAAAAACATCTGTATTTATACCAAATTTAACAACTTTGTAAGGAGCTAAATAACCATCTTCAATACCTTCTTTAAGTGAATATGTATAAATTGGTTCACCAAAATAATCTATATTTGATACTTCAGCAGTCTCTTTAGGAGTTGCAGTTAATCCAATTTTAGTAGCAGAATCAAAATAATTTAATATTTCTCTTCATTTAGAATCTTCACTTGCACTACCTCTGTGACATTCATCAACAATTATTAAATCGAAAAAGTCTGGTTTAAAGCTTTCTTTTAATTTTTTGAGTGAATCTTCGGAGCCATTTTCAGATAATTGTTGATATAGAGATAAATAAATTTCATAAGATGTGTCCATATTTCTTTTTGAAACTTTAACCATACTATTTTTGAAAGGTTTAAAATCCCCTATTATAGTTTGATCAATCAAAATATTTCTATCAGCTAAATATAAAATTTTCTTTTTTAAACCAGATTTTCACAATCTCCAGATTATTTGAAAAGCAACATATGTTTTACCTGTGCCAGTGGCCATAACTAGTAAAATTCTATCTTTTCCTTTAGCAACAGCGTCTACTGTATCATTTATGGCTCTTTTTTGATAATATCTAGGAATATGAGAATTTTGTCCATAATAATAAGGAGTGGAAATTATTTTTTCTAAAGCCTTATTTTCCTTGATTTCTTCACCATATGTCATCTCAAATAATTCATCGGGCGTGAAAAATTCATCTAACCCTATTGTTTTCTCTATTCCTGTTTTCATATCATAAAATCGAAAATTGTTACCATTTGATGTAAAAACAAAACGAACGCCCAACCTTTTAGCATAATTTTGAGCTTGGAGTAATCCGTCGCCTTCTTTATGATCTTCACCTTTTGCTTCAATAATTGCGAGCGGTGAACCATCTCCATTGTAATTCAACAAATAATCAATTCTTTTTGCTTTTAGTTTTTTAGCCTCACCATTTTCGAAAATCACTTTTCCAGCAGAAATATAATATTCCATATTTATGAAATTTTTATCTCAACCAGCTCTTTCTAAAGCTGGTGTTATAAAACGTAATTTTACTTCTTCTTCATTTATAGTATTTTTTTTATTCATCTTAATAACCTCATTTAAATAAATTCCTATAATATATAATTATAAGAAGTATTATTATTAATATAATTTTATCATTTAAAGGACAAAATTAGAATGATAATTTTATTAAAACATTTAAAATATAAAGAGAATATACAAAATTATCAACACAAAAAAACTTTTTATTTTGTAATAAAATAACTTGTTTTAAATTATTTTTTTGAAATTTTCTATTTTATAGTAACAAAAACATCAAATGGTTAGCCACACTAACCAGTTCATTTTTTTCTTATTCAATTTAAAAAAAGATAAAATAAATATATGAAAAATTCAATAGATCAAAAAAGTAAAATAAAGGCTTATAACTTATTTGACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCCGAAAGTAATTTGGAGCAAATAATAGATAAATATATTGAAATGAATATAGCGCATCCATTTCTAGAAGGCAATGGACGAAGTACTAGAATTTGATTAGACTTAATGCTAAAAAAGAATTTACAAAAAGTTGTTGATTGAAGTTTAATAAATAAATTTGATTATTTACAAGCTATGCAAGAGAGCCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGGATTGATTATTCTTACTACTATGAAGAGTAATCTTTTTTTATGTTTCAATTTATTTAAAAGACCTGTTTTATAGGTTTTTTAGTAAAAAATCAAATATTAAATAGTATCTAAATACATTTGATTATTTATGTCTTCTAAAGGTTCTCTAATTTCCTTTTTAGGTACTAGTCAATGGCTTGAAAATAAGAAATTTAAAATAGTTAAATTTATAATTCCGGTAACTATTATTCAGTCCATTCTAATTTCAACATTTGAATAATCATCAAATTTGAAATTATTGTCAACTAAATAATTAATTTGGTCATATTTGTATCATAAATCTCGATAAAAAGGATTGCCATAAACAAAGTAAATTAATAATGACTCATCAGTAAATTCACTAAGATAAAACAACATATCATGTCAAGGTTTACCACCTAAAGAACTTTTAGATATATCTTCATTTCTAACTAAATCACATAAATTAGCAAGAATCATATTATTTATATCCATTAAATCTTTATATAATCTGGCATATTTAATATTTGCTAAATTACCTTTTAGTTTATATCTATAGTCACTTTTGTCACCTACTTCTTTATTTTTAATTGCTTCTTTAACTTTGTCGAATAATTCCTTAGGTTTAATTTCTTTCATACAATTAATTGTTTGAGTGTAATACTTTTTAAAGTTTTTGATGAAGAATTCAGTATTAAATTCATTACATTTAGCTTCTTTATTACTATCAAATTTATTAAGAACTGCTCATTTTATATGACTATATTCAATTTTGTCTAATGTGTTTTCTTCTAATTTCAAGTAAGAATTTAAAAGCTTTTTTATTTCTGAATTAGCAATTTGTCCACTATTAAATTTAGTTTCACTTTTTTTGTGTTGATCATAATATCAAGAACCAAATTCTTTTAAATCTTCTTCATAAAAAGTGTAATCTAAAAATAATTGTTCTGTTCATTCTGGAATTAAATGAATTTCAACATCCTTGTCACTAATTACATTAACACGTGGAATATCTATTTGAAAGTTTTTTCTTCCTAAATCAGCTTTTAAAGTTTTTTCATTATTTAGAAATTCAATAAAGTCTTCTCTGATAGTTAAACTATTTGCTCAATTAAGATATCAAATACTTTTACCTTTTGAATCACAAAGTTGATAAGCCACTACTTTAGATGTTTTTTTGTTGCCTATTTCGCGTTTTATATAATTTCTAACTTCTTCATACATTTCTAATTCATATTCTTCCATGATGTCGCAACCAACTTCATCATTAATGTCATCTGAAGTTATAATATTAAATAGTTTTTTCATAATTTACCTCTCTTTTTATATAAATTTTAAATAAATTGCAAAATATTTTTTAGAAAAATACATAAATAATCAAAATTTAAAAAACAAAAATTTAGATTTTGGATTATTTTAATTTGATAAAATAAAATTATGAAAAGAATAAAGATATTTGACAAAAAATTAGTAGAAAATTATAAAGATACATATTCTTATTTAATTAAATGAATTGAAGAAAATCCGCATGAATTTTTAGCTTGCTCTCAACATCAAATTACAGAAAAGATTTATGTATCTCAATCTTCATTATCTAGATTTACAAAATTTTTAGGTTTTGAATCATATCGTGAATTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAGTTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCCAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATAAATTCGATCCATGATTTTAAGGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGATAAAAGCAATTTTAATTTTCAAATATTGACAAGAAACGAAAATGTTTCTTATGCAAATTGCACCTCACCAATAATTTATGATTATATTCCTGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTCATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAATTAGTGAATAAAAAATTCAAAAATAGTGAAAAAAATTCGGTTTTTTTCATATTTTTTTGCATTTTTTGACTTTTTATATTTCATTACTGAAATTAAATTCCTAGAAAAAAGAGGTCAATATATATATTGATAGAATAAAGTTGAGGAGGGCAAGTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTAAGTAATAATTACAACCGTAATTTTGCTTGACTTTCATTAGCTATTTTTGCAATCGGATGAGTTTTGTGAGAATTCGCTATAGCTAAAAGTGGTAATAAAAATAAAGCTTATGCAGCTATTGCTTTTATAGTTGTTGGAAGCACTTTAAGTTTAAATATCATTAACTATATTTGCACTTTTATTGCATTAATCATAATTGTGCTTTTAACAGTTACGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGCTGATTTAGAAGACAACAAGAAAAAAATACAAGAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGACGGAATTTAAGGAGATATTATGAGATTTAAAACAACATATGCAGTTTCAGCAAATGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGCGGAAAGCTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACGCCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTCTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTTAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGCCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAAGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGATTACAATTTTAGAACCTAACAATATGTGAAACACAATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGTCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGAAGTATTCACTCAGGAGTAGGAACAAGTAACTATGCTTTTATTTGAGCAATGGGTGGAGAAAATCTTGACTATACAGATATGCAAGGTGTTAAAACAATAGATCTAAAATAGTGTTTAGAAATCTAAATAATTCATTAATCTATGAAAAAGAATTCAATGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGAACAGATGGATTTTGAACAGGAATTCTTTGATTAGCTTATGAATACACTAATAATAATAAATATAAAGAATTAGCACTTGAGCATGTCAAATGATACAAAAAAAGAATTAAAGAAAAAATATTGGTTGATCATCATGACATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGAGGAAATATTGGGGCAAATGATAATTATCGTTTAATTATTGACTGCTTATTAAATATTCCTTTATTATATTGAGCTTCTGATTATGATAGAAAAAATTATTTCGATAAAGCATTAAATCATTTTAATACTTCTTTGCATACTGTTATAAGAGAAGACGGAACAACATATCATACATACTTTTTTGATGCTAAAACAAACAAACCTTTATGTGGTAAAACTAGACAAGGTTATAGTGATGACTCTTGCTGAGCAAGAGGACAAGCGTGAGGTATATATGGAATTCCACTTACATTAAAATATGCAAAAAATAATTCAAAAATAGATAAAAACGCTTATCAAATTTATCAAAAAGTGTTAAATCAATTTTTAAATAATTTGCCTCAAGATGATTATGTTTGTTACTGAGATTTAATTTTTAATGAAAAGGCAGATCAATCACGCGACTCATCAGCTAGTGCTATAGCAGTTTGTGGTATTTTAGAAATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGACATTCAAAAAAAGGTGTTGATGAAGGAAATATTTGAGGTGATTATTATTACTTTGAAGCACTAATGAGACTATATAAAAAGGATTGAAATCCTTATTGATAAAAGGGAGATTTTATGGCAAAAATTGTTCAATGTAGAGTTGATGAAAGATTGATGCATGGACAAGCATCTATTTGAATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGACCTAAAGCTGCTGATTGACAATTAATTTACATTGTTGTTAAAAGTATCGATGATGTTTATAAATTAGCTTTAGGAAAATTACCAATTGAAGAAATAAATATTGGTAATGTTCATACAAGAGAAGATCGTGTAAGAATTACACCTTCAGTTAATCTAAGTAAAGATGAAAAAGCAAAAATTAAAGAAATGATTGATTTAGGAATTAAGTTTAATACTGCAAGCCTTCCTGGTGTAACTAAAGGTTTAGTTGACCAAAATAAATTAATTAGTTAGGTAGGACAAAATGGAAATTTCAATTATTCAAGCTATCTTTATCGGAGTTTGAGCCGCGGTAGCAATAACAGGTACTATGATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGATTAGGCTTTGGTATTTCACAAGGTGGTGTTAGACCGCCAGATCCAATTGCACCAGGTATTTTTGCCACAGTTGTTGCAATTGTTGAAAAACACGGAAGCGCACAACCTATTTCAATTGGGGATGCTGGTATTTATATTGGATATTCAATTCCAGTGGGAATCTTAATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGATTAAGAACCGGATTAAGAGTTGCTGGTGGTATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAATAACAAAGATTTTGTGCAAGTTAATCAAGCTCAAACTAAAAAGCTTTCAATTTGAACTTACATTATTGTTGCTTTAAGAAGTTATTTTCTTCAATCAGGCTTTAACTATGCTAACTTTCAAGGTTTAGGCTATGCCAACATAATTTATCCTGCTTTAAAGAAAATTTATGGTTATGGAACTCAACAATTCAAAGATGCTTTACTAAATAATATAGAATTCTATAATACTAACCCTCAATGTATTCCGCTTATAACTTCAATTCACATTCAACTTTTAAACAATGGGACAAGTTGAGCAGATGCAAGAACTATAAAAATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATTGGTTTTGCTCAAAGCGGAAATATACTAGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATCAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTTTAGACAACATTATGCCACTATTTGCTTCAGGAATATGAGTTATCTTTATTTACTTTATGGTAACTAAATATAAATGAAGTCCATATAAAGTTATTGTAGTAACTATTTTAGTTGGTGTTACTGCTGGAGTATTTGGAATTTTAGGTGTTTAATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGGTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAATTATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGACGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAATGGGAGACTATCATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAGGACAAAGTTGTTATTATTACAGGTGCAAACATGGGATTAGGTTTAGCTTATGCTAAAGCTTTATCAAATTGTGGCGCCAAATTAGTTATTAGTCATTTTGACACAAATACAAAAGAAATAGAAAACTTTTGTGCTAAAAACAAAACAGAATTATTGCTTGTTCAAGGTGACTTAACTAAAGAAGCAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGCACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGAAATAAAGTTATTGATATAAATATGAATGCTGTTTGATTCTTAAGTCAAAAAGTAGCTCAAATAATGAAAAAGCAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGAGCAAAACCAGAAGAAATTGCCGGTGCTGTTGTATTCTTGTGTTCTAAAGCAAGTGACTACATCTCAGGAGCAATAATTCCTGTTGATGGTGGTTTCTTATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGACTAAAATCAGGAGCTAAAGCAATAAGTGTTGGCTCTAACTTGTTAGCTGGACAAAGTGAAAAAGAAAAACTTGAAAGTATTAAGAAATATTTAAAAATAGTAAATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGAAATAACAAATATGCTGATTTTAAAAAAGAAATTGATCCATTTTTAAATTTGTCTGATATTGTTTTTGGTTGAATTTCAGAACAAAATTATTGCAAAAGTAATAGTTCAATAAGTGCAAAAGATTTTGACAAATTAACCTGTTTAATAGATAAAAAATACAAAAATATCGAATTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTAAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAAATTGAAAGGTAAATATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATGGATCATCCTCTTGCTAACTTATATTATGCTGGGACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAGTAAAAGCTAAAAAAATTTTTGATCGAGAATTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGTCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCTGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGCAAAATTAAAAAATATTATTGAGAATGTTTTAGATGATTTTGCAAAAAATTATTATTACAGCGGACAACAACAATATGTTAACTGATGATTTTATGAAATCGGTATTCCTAAGGATTTAACAAAATTATTAGCAGTTTTAAATAACGTTTTTCCTAAGGAAAAAATAGAAAAATGAAAAGCTGGTGTTGATTACTTTTTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAGCCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGACGGTTTTTATGTTGATGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTCCTATATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCCTAGAAATAAGGAAGATTGAGAATTTTATGAAAATAATTATTTTGATCAAAGAAAAGAACCAAGCTCAACAAATGAGCATTCAGAAAGCTCAGACAATGCTTTAGTTGGTGTTGATTTACATAAAGAAAACGATGGTTTAGTATTTACAAAAAATCAAGATAGATATGTTTACAAACATGATGATTTTATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTTCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGACAAGCAGTTAATACATTCAAAATTCCTGGCACAACTTCATACATCTCCTCTGCTTACGATAATATATATGATTATATTTTTCCATCTTATATGCCAGTCGATATTGAAAAATGAAAAGAAATAGGTGAGGTCGAAATCAAAGATAAAGAAGGAAATCCAATTCTTGATGCAAATGGTAAACCAGTTAAAAAAACAGGCAAAGAACTTTATGATGAGTATCAAAAATTTATTAAAGAAGATTTGAAACAAAAAGAAAGAGACATTGAATTTCAAGTTTCAACAAAAACTAATAAATCATTTAATAATGGAATAATTTGAAACAAAATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACGATTGAAAATAGAATGATCATTGATAAAAACACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAAAGGAACAAGATTTATTTATTTGAAAAAATAAATTAAATGAACAAAATGGATATTTCATATTAGGAAATAATAAGGCAAAAGTTGAATATTTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGACAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTCATAGCTTTAAAATATCAAAATAAAGATTCCTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATATGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCTGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGATTTAGATCTAAAAATTAATTTATGAAACCCTACTACAATGATTATTAAGAAAGTTCAAAATTCTTCAATTTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTATCAATTTAAGCGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGATTTACATTAACAAATTAAAGGAGTTATTATGAAAAAACATAAAATTATATTTGCTTTAACTCCATTCACTCTCATGCCTTTAACTGCAATAAGTTGTAGTATTGAGAGTTCAAATGATTATGAAAACGCTAATAAGTGATCTAAAGAATCAAATAAACCTGGAATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGTGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTGGTTCAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTTAGATGAAACAACTACAAATTTTGACCAAAATTCTTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGAAATAAAGGTGTGCAATATTTGCCTTCATGAATACAATATGATGTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAACTAACGCTATTCAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAGTTAGAAAGGAAACAATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATACTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGAGATGAAGAAAAAGGAAAAGTTTCTTTTGAAATAGCAAATCTAAAAAGAAAACCAAGTACAGTAATAGAAAGTGAAATCGAAATAAAAAGACCTCAATTAGATTCTTCAATAAAAATTAATTTAACTTTAGAACCAAATGATGCTGAGAAAAAATTAGTTATTAAATGAAAAATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGATTTAATAAATCAATTAAACTTCTTGAAGCTGAAAGTAAAACTAATTCACATATTGAAGATATATATCCAGCTGTTCAATCACTTAATTCAATTTTAGCTTCTTATGATCAATTAGAAAATGATGAAAAAACTAAAGCTGATGAAATTATTCCTGCAACAATAAATGCTTTAGCTGAAAAATATTTTGGAGCTAACCAAGTTATTTATAAAGAACATTATTTCTATCAAGAAAAAATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGAGCTAATAAAATGTATTCAATAACTGTTTCTAAATATGTTATAGCTTTAGCTAATAAAGATAAAGACGAAGCAAAAAACGTTCTAAAAGATTTTTGAGAATGAACAAAAGATAAAAATGCAGCAAATATTTCATTAAAAGAAAAATCAAGTTATTACTTTAATTTTGACAATAAAGACAATAACAAGAGATTGTATAATTTTTCAAAACAATTCGATATGCTTAGAAATATCAATAGATTGTTAACTAAAAATGTTAAGAATACTGAATGAGGTACATTATTTGATTCTATTAACAATTACATTAATAATGAATTAATTGACTGAATCAAAGATACTTCAAGCTTAGTTAAACTTTCAACAGGAATTCAATTAATTGGCTCATTTATTAAATTCTTAGAAACTGAAAAAGATAATGTAAGATTAGAAACACTAAAAACTAATCTATTAGCAAAAGAATTAAAAAATATAACAGGCTATGATCAAAAACAAGGCGGTTGATCATGAGCTTGAGATGCAGTACATAATTTACTTGCTTAAAATATTTTAATATCACATTCATTTGTGATATTTTTTTAAACTTTTATTATAAAAAACCAGCCTTTACTAAAAGCTGGATCATAATATTAAAAACTAACTAATTTTCTTTTTAACTAATTCAAAATATACACGAATAATTTTCGATAGTATTCTCTTTTACTACTGCTTAAGTCTGAAGGTAAGTTATTAGTTGCTTTTTGAGTTATGCTTTCATATATTTTTTCTTGTTCTAATATTTTTTATCAATACAACTTAGAATTGAGAATCAATCCTCCATACTTAAACTTTGAGCATAGATTTCACTTGGTTTTTTTGTTATTTTTAAAGTTCTTTCTTTTTGTTTAATTTACTAAAAATATATTAGTTTATAAAACTAAAAAATAAAAACTTAATTAAATTTTTTAGTAAGTAATCACAGAAATTACTGCATTATTTCTTAACTTTAATTTATAATTAAATAACTAAAAAATAATAAGGATGGTCCAAATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGAGGTGTTAGAATTGATACTTCTAAAAATATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAAAAAATAATTTTTACTTAAATATCTATAAAACAGGCTTATTGGTCTGTTTTTAAATTTAATTTTTATTAAAAAATGTTAAATAATTTAATAATAAAGTATAATTTAAATATAATTTTTTTGAGAAGGGAGCTTGTAATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGAGTTTTAGCAACAATATTCCTTATTTGTACAATTGTCTATGCAGTTAGATCTAAATAATAACTAAGCTTGCAAGTGCAAGCTTTTTTATATTCTTTTTCTTAGAAAAATAAAGCTTTCTAGTATAATTTTGATACATATTTTAAAACTAAATGAAAAAGGAGAATTTATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGAGAAATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAATAATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAATAATAAGAAGTTCTCAGTAAGGGAGCTTTTTTATTTTGCTTTGATGATGCAAAATAAATGTTAAATAAAAAATAAGAGCAATTAAGCCCTTATCAACTTTTTAATTAATACAATTTCACACTAACATCTTGTCTATCAGCTTCTTTAGCTTGGAATAATAGAATTGTTGTTAATCTCATACTTGAAGCAACAACACTTTTTGGATATGTAAAGATTTCTTGGTTAAATGTTGTGGCTGAAGTATTGTAAATTCTTCTAGCTGAAGCAATGTTGTTTTCACATTCAACGATTTCATCATTTAAACCTTTAACTAATTCAGCACTCTTAAGTTCTGGATATTTTTCGAATTGAGCCATAAATCTTGTTAAGAATGATTCTGTTTGACTTTCAAAGTTTTGACGTACTGCCATAGCATCTGCTGAAGTATTTCCTGATTGTTCTCGAATTGTAGCAACTGCTGAACGGTATGCTGCAACTTGTGTCATAACATCTTTTTCGAATTTCATGTGTGAACTTACTGATTCAACTAACTTAAGAATTGTTCCTGCTCTTTTAGCCAATTGAGTATCAATTAATGAAGCATCGCTACTTATTTTCATTTGTTGTCTTCTAAAATAATTTACTCAAGAAATATGAATAAAAATTGGAATTAAGAAAATAAATGATATGTATCAAATAAATTTTCCAAAAAAGTTTACTTTAACTGGTACTTCTGTACTCATTGAACGTGGTTCATAGCCTTTTTCATTAATAGCACGACTGTTTTCACGATTGTATAAATTAGCCATTTTTTCTCCTTCTATATAATTATTTTTGTGTTCAATACATAAAAATTATATAAGTTTTTATAGTTATTAAAAACAAAAAGTTAAGCTATGCCAGCTTAACTCGTTTTTTAGATTCATATTGATAATCAGTGGCAAATAAATAACCATCTTTAAATTTTAATTTAGGTACATTTTTAATTTCTTTTTTGGTTGCAAAAAAGATCTTAATTCTTTTATTATCAATAAAAGTGTAAGCACCAGGATTCATCTCATAAGCTCTAATAATATTGATTGCTTCTTGCATGGTTAAGGACTCATTTAAAAGAGCTTTTTCTTTTTCTAATTTGGGACTTAAAGTTACTTTACTTTCATCTTGTTTAATTCTTTTAAGTTTGTTTTGATCTAAATCATTTAATCATTGCACAATGTTATCTGCACTAAGCTTTGAAAGCTTATTAAATAAAGTACTTGCTGTGTCATATTCATCTATTTTAATGGCTTTTTGGACAAAAACATCACCTGCATCCATTTGTTTAATCATTTCCATTAGACTAATACCAGTTTCTTGATCATTATTTAAAAGTGAATATTGAATTGGTGCTGCTCCACGATATTTTGGAAGTAAAGAACCATGAATATTTAGACTTAATTTTTTAGCAATATTTAAAACACTTTCAGGAATATATTGACCAAAGGCACAGGTAATTAAATAGTCATAATTTAAAGTTTGTAATTCATCTTTTATTTGTGATATTTTTTCTGGTTGAAAACATTTAATGTTATATTTTTGAGCTAATAATTTTGTTGGCGTTGGAGCTAATTTATGACCTCTGACACTTGGGCGATCAGGTTGCGAAACAATTGCAACAACTTCAAAGTTGTTGATTAATTTTTCAAATATTTCAACTGCAAAAGCAGGAGTTCCGGCTAATAATAATTTCATATTTTAATTATATAAGGAAAAATAAAAAGTGGAATTAATCCACTTATTTTAATTATTCTTTTGTTCTATGTTCAATGGTTGCTGGTTGATTTGCACTTTTTAAGAATAAATGTAAGAATGTTGAAACTAATGTTACTGCAAATGTTACAAATAAAAAGAATGCAAATGCAACGTGAACGTTAGCATATGATTTATCATCTAATGATCTTGGTCCTGTTACACTTCAAATATTCGCAATAATTCAGCCAACATAACAAATAATTGTTAAAACAACTGCCGCAAATACCAAAATATTTAAAATAATGCTAATTACATTTTTCGAAGCTATTGATTTTACAAATGCTTTAATTGCATAAACAAGAATAGCAATCATTGTAATATGAATGATTATATGTGCTAATAATGAAACAACATAAATAATTTTTACATTATTGCTATCATGTTCAAATATACCTTTAATCACATCAAAATTTTTAAATCATAAGAAGAATCCATAATTTAATCCAGAATGATATATTGCTTCGTATTCATTAAAACTAGATTTTGTGTTTTTTACGATAATTTGATGCAAGTCAACAGCTGATTTTACTATTAAACCAAAGATAACTAAACATGAAATTGCTAAACCAAATAGAATTCATGATGTTTTAGTTAATTTTGTTCTTTTTGTGTTTTCCATTTAATGCCTCCATATAAAAATTGTATAAGCAAAAATTTGAAAATAAAAACAAAAAAATAAAAATAATTTTGACTAAAATGTATATAAAACAAGTAAATAATAGAATTATTTAATAAAAAATAAGGCTTTTTAAAACCTTATAATTTTTTTAAGCTACAACCTTTTTACGACGTTTAACAATAAATCTTCAAAGAGCAACTAGAGCTAAAGCAAGAGGTAAAAGAATTGTTAAAGCAACTACTACTAGATAAATTCTAAGTCACATTTTGTTACGATCTTTTTTGCTTTGCTCAACTTGTTCATGAATTTCAACAGAACGTTCACCGAGAAAATCACTAATGTCAGCTAAACGTTTTTTATCAAATAATCTTATTAAAATATACGCCGCTATTGAAGAAGCCATAATAATAACACTAATAATACAGAATAGAATTGTGTTGCCAAAACCTCTATCAAGTCATTTAGCTCATTCAAAATTGATTTTTAATGTATCAGTTTCATAATATCAACGATTTGACATATAAAGTCCATAAACTATTGCTGTAATAATGCCTAGATATGTTAAGACAAAAACAAGCATTCAAGTTAAATTAACATCTTTAAGTACTATCTTACGATAAGCAATATGAAAAGTACTACTTGAAGTGTAATCACCATTACTTACTGCATCACGATATCTTTGAACTGTGTGAGATCATTGAATATTTTCAATCAGATTTTTAAAACCAATTGCCATTGAGATAAAGGCACTTGATCCAAAAAGTACAAGGTAGCCAACTAATCTTTGTTCTTTGTTGAAGATACCAACTTTAGCATAGACTAATGTGAAGAGAATAATTGAAGCAGCCATAATTACAAATGAAATAGAAAGGATTAAAATCTTAAGCATTTGTTCAGCACGGATGACGCGATAAACACGAGGTGATATTTGATTTTTTGGATCACGATAAACACGACTACTTACACTATTGAATTTGTAGTCGTGGTTATTTTGAACAATTTCGATACTTGCTGAATTTTGAATAGGTTGATATTTTCTCATTCTTGGCCTTTCAATTTTAGTACTCTAATTATAAATGATTATTCGCTATATTTATTAATATAAATTTTAATATTTTATAATTATTTTCAATAATTAAATTTGTGAGATTTTTCTCAACAATTTTTCATTTAAAATTGATGGATTTTACTAATAAAGCTTTTTTATAGATTGATTGGGCAAATATATAAAAATTGTGCCTATGATTAAGCACAATAATTATTTGATTATTATTTGTTTTTGTTTGAAGGTTCTAATTCTTCTTTTTGTTCGTCTGCAACAAGTTTTTTAGCATCTGCTAATTGAATGCTGAAGAAGCTTGTAACACCACGGTTTTGCATTGTTGCACCAAATAATGTGTCAACTCTTGACATTGTTCCATGACGGTGTGTAATAACCAAGAATTGAGTTTTTTCTTTAAGTTGTTGAAGGTATTCTGCATAACGAACAACATTAGCTTCGTCAAGTGCAGCTTCAACTTCGTCAAGGATACAAAGAGGTAATGGACGAGCTTTAAGAATACCAAATAATAATGAGATAGCGATAAGTGATTTTTCACCGCCTGAGAATAATTTAAGGTTTTTAATATTTTTTCCAGGAGGTTGAGCTTCGATTTCAACACCTGATTCTAAAATATCTTTAGGGTTGCTGAAAAATAATTTTGCAGTACCACCACCGAACATTGTTCTAAAGACATTGTTAAATTCATCATTAACATCATTAATAATGTTAGTTAGACGAGTGATAATGATTTTATCCATGTCTGCAATAGCGCTTTCAAGTGTTTCTTTTGCAGCACTTAATTCTCTTTCACCTTCGGCATATTCATTGTAACGTTTTTCTTCTTCAACTAATTGTTCAAGAGCTTCTAAGTTAACGTTACCTAAGCCTTCAATTTCTTCTCTTAATTGACGAACAATTTCTTCAGCTTGATCACGATTCATTTCTAAGTGATGAACTTTTTCAGCTGCTTCAAATGTCATTTTGTATTGAGAAGCAAGTCTTTCTCTACTTTGTTCAAGTAAAGTTTTTGATCTTTCTCTTTCACCAATTTTAGCTGAGAAAGCATCTGTTAATTTACGACGTGTAGCTTCATAATCATTTTTAAGAGCTAAAGTTGAATTGATTTCGGCAGCAATTGTATTTACACGTTCATTTTTAGCTCTGAATTGTGATTCAAGAGCAATTTTTTGACTTTCTAAAACTTCAATTGAAGCTTCAGGGCTTGATGCTGAAACACCTTCTTTAACTTTGAATTCTTTGTTTGTAATATTTTGAAGTTTTAATTGTAATTCGTCGTAAAGACCTTGTTCACTGCTCTTTTGGGTTTTTAGACTAGTAATTTGTTGTTTTAATTGGCTGTCTAATTCAAGAGCACTACGACGATAGTTTCTTATTTCATTGATTTTTGATTCATCAGCTTGAACTGCTGCTCTCATACCTGGAACAATATTTTCAAGCTCTTTAATTTTTTCATCAAATCCAAGTAATGATTGAGCTGTATTTTTAGCTCCACCAATAATCACACCGCCTGAACGAATGGTGTCACCATCAAGTGAAACTACCATGTAACGACGTTCGAGAATACTTGAAATTTTGTTAGCATTTTCAATGTCGCTAGCAACAATAACATTACCTAAAAGGAATTTATTAAGAATTTCATATTGAGGATCAATTGTTACTAAATTACTTGCAACATCAATAAATCCAGGGTGTCCTTGGATTGCTAAAAGATGATCATCACGAATCATTTTTGGTTGAATTGATGTGAGTGGAATAAATGTAGCACGACCACCATTATTATCTTTTAAGAAGTTAACAGCTTTAACTGCTGTTTCAGCTTTATCAACCACTAAGTGTTGTGAAGCATTAGCTAAAATTGAGTCAATAGCAGGAATATATTCTGTTGTAACTTTAATTAAATCAGCTACAGTTCCTTTTAGTGCTTTTCCAAAGTAAGCTTTATTTTCTAAAATGTTTTTGGTCCCTTTAAATAATAAAGAGTTACTTTCTTTTTGTTGGTTAAGCAAGTTTAACTGTGTTTCAATTTTTAATAAGTCTGTACGTTTTTTGTTAATTTTAATGTTCATTAAATTAATTTCTTCGTCATATTTTGAAATTGTTTTTTGGTTTTCAGCAATTTGGTTTGCTTGATTTTCAATTTCACGAGCGTAGTAATTTAATTTATTTGCATGTCTTGTAAGCATAGTTTTGTATGCTTCGATTTGTTCAGCTTGGTTTGCTTGAATTTCACCAGCAGCAATTAATCTTTCACGTTCATTTTCTTTAGCTAAAGAAATGTTTAATTTGTTTAATCTTTCTTTAACTGTTTCATATTCAATTGAAAGTTCTTTTAATTCGGCTTCAACTTCTGATCTGAATTTAGTATTTTGAGTAATAGCAGCTTCAGCTGATGAAATACGAGCATCAAGATCATTTTTAGTTTCTTGAACACCTTCAAGTTCAGTTACAAGTCTTTCGTACATATCACCAAAAGTTTTGATGTTGTCTACTAAAAGACCAACTTCAACAGCTTTTAATTCTTCACTTTTAGCAATGTAAACTTTAGCTTTTTCTGCTTGTTTTCTTAATGGAATTAATTTTCTTTCAATTTCACTAATAACAAGTTTAATTTGGTCTAAACCTTCTTGAGTTTTAGCCAATTTGCTTAAAGCTTCTTTTTTACGAAATTTGTATTTTGAAACACCAGCAGCTTCTTCAAAAATAGACTTTCTTTCTTCAGGTGTAGCTTCAGCAATATCACTAACTGTACCTTGAGAAATAATAGCTAATGAACTTTTACCAATACCGCTTTCCATAGCAATATCTTTAATATCTTTTTGACGACATGGTTCGCCATTTAAGAAATATTGGTTGTTTCCATTTCCTCTTTCAAGCACACGACTAATTGAAATAGTTTCATGCGGGATTGAACTAATACCATTTCGATTATCAAAAGTTAAAGTAACTACTGCTTTATCCATAGGTTTTGCAGTTTTAGAACCAGCGAAGATAACATCATCCATGTTATCACCACGAAGTTCTTTAGCACTACGTTCACCTAAAACTCATTTGATAGCATCGTTAATATTTGATTTACCAGAACCATTAGGGCCAACAATTCCGGCTACTCCACCATCAAATTTAAGCACAATAGGATCAGCAAATGATTTGAATCCGTGGGCTTCAACTTTAATGAGTTTCATATTACCTCCTTTATTTTTTGAACCACTCAATTCTTGATTTTTTGACGGTTCATAATTATAATTTTAATATTAATATCTAATTTAATTGTAAAAATTTTTATTTTTTCATTTTATGCAATGCATCTAGTGCAGCATTTTCTTCGGCTTCAGCTTTGCTTTTACCAAAACCAATACCATAAATTTTATTGTCGTGAACTGCGTGAGCTTCAAATTCAGTTGGACTTTTTTCACTAACTATATACATAACAGCTAGTTTACTGAATGATTGAATTTGTTCTTGAAAAGCAGTTTTAGCATCTTTAAGATGCTTGATGTCAAAGTCTCCAATTTTGATATCAAAAACAGTTTTGTTTAAAAATTCTTTGACTACTTTTAGATTTGTATCCAAATAAATAGCAGCAATAAAGGCTTCAAAAATGTCAGCGCCAACTTTAACTGAGCGTTTAGCATCTTTACTCATTTGACCAGGTGCAGTTAGTAAAAAATCTTTTAATCCAATTTTAGTGCTAATTTCATTTAGTGACTTTGTGCAAACTAATTTAGAACGAATTAAAGTCATATTACCAGCACTTAAATTTGGAAATTTTTTGAATACAAAATCTGACGAAAGATACTGTAAAATAGCGTCTCCGAGAAATTCAAGATCTTGATAATTGCCAACTCTTTCACTCACATTATTTTTGTTTTGATTTTTTTTGAGTCCAAAATATGAACCGTGAGTTACAGCTTGACGATAGTATCCTAAATTGTGATAATTGATGTTTCAAGCTTTTAAAAATTCTTGAATAGTTTGTTTAATTTCATCCATTATTTATTATTTTTCTTTCTACTCTTCATTAATATTTAAGTCTTTTTTAACCTCAGATAAAATGTGGTTATCTAAAGCTAATTTTATTTGATTAAGTGCTCCATAGTAAGCTTTTTCATCACTACTTCCATGGCTTTTAATTACAACACCATTAACCCCAGCGATTCAAGCAGCTCCAACATTACGATAATCAAGAGTTTCAGCTACATCTTTAAATGCACCACGAGCTAAAAGGTAACCTAATTTTCTAATTGGTTTCAATTTAATTTTGCTAGTTAATAAATTTTTAAAACTTAAAATTGCGCCTTCAAGACTCTTAAGAATTAAGTTACCACCATAGCCATCGATTACTGCTACATCACAAACACCATTTAATAAATCACGAGGTTCTTGAAAGCCAATGTAATTTAATTGATGTTGTTCTTTAAGTTTATTATTTGCTTCTTTAATTAATTCTGTTCCTTTATAGTCTTCAGTTCCGATGTTAATTAATGCACAACGAGGTGCTAAGTTTTTTAATAAAACACGATCAAAAGAATTTGCAATTTTAGTTCATTCAACTAAATAATCACTTGTGGTTTCTAAGTTAGCTCCAACATCAAGAAGTAAGAATTTACGTCCTGTGATTGTAGGCATTAAGGGCATAAATGCTGCTCTTGAAATGCCTTCTAATCTTTTAACTTTAAAAGTAGCAGCTGCAAGATATGTGCCAGAGTCTCCTGAAGAAAGTACAGCATCTGCTTTTTTATTTGCTACTAAATCAAGTGCAACATTCATTGAGGTATTTTCTCTTAAAGATTGGCGAATGTTTTTAACATCGCTTGGCACTGATGAGTTGTCAATTATTTCTAAATTTTCTGGCATGTTTTTGCCATAATATTTTTCAATTTCAGCCTTAGGACCAACTAAAATAATTGCATAATTTTTGTTGGTTTTTAAAAATAATTTAGTTGCTGCTAAAGCTGAATTAATGCCGTTGTCATTGCCATTAATATCAAATGCTATTTTGTACATTTTGCTCCTTATTTTATTCAAAACCAATAATGAAGTGGTAGTTAGGTTGAGCACCATTCACTACTTCAACTTCAACATCATAATGGCTTTCTAAAAAGTTTTTAATTTCGTTAGCATCAACTTCACTTGCATCATTTCCATAGTAAACTGTAACAAGTTCATGATCACTGTTAATTGATTTTTTAATCAATTTTTTAGCTGCATCAATGTAGTTATTTTCACTAACTAAAATCTTGCCATCAGTAATGCCAATGAATTGACCTTCTTTGATTTTAACATTGTCAATTTTTGTGCTTCTAATTGCTTGGGTTACTTCAGCTGTGGTTACAAGCTTAATAGATTCAGACATTGTTTCACGGTTTTCTTTAGGACTTGATTCAGAATTAAAGTTCATTAAAGCTGTTAATCCTTGAATTTGACTTCTAGTTTGAATAATAAATACCTTTTTATTTGTAATAGCTTGAGCTGCTTGTTCAGCTGCTAGGAAAATGTTTGAGTTATTAGGTAAAATGAAAACGTTTTTGCTATTTACTGAGTTAATTGCATCAATAATATCTTGAGCTGAAGGGTTTTGTGTCTGACCACTTTCAATAATAGCGTCAACTCCAAATTCTTTCATTCTTGCAATAATTCCACTGCCTAAGTTACATGAAATAATGGCACATTTTTTGTTTGATTGTTCATCATTTTGGTTAATAATAACGTTTTTATTACGACTTTCACTTGCTTGACGAGTCATATTTTCTGACTTTATTTTAATAAATTCACCATATTTTTGACCAAAGTTTAACATATCACCAGGTTTTAATGTATGACCATGAACTTTAACAATTTTTTCATCTTGAACTACAACTAAAGAATTAGCTTTTTTAAGTAATGATTTTTCAAAATTAGCTTTATCAAAGTCTTTTTCGTCATTTAATTCAATAATGAATTCAGTACAATAACCAAATTCGCCATCATAAACTTCTTTTGAGCTGATAAAAGTATCAATGTTTTCTTCTGATTCACTAATTTTAATGGCTTCAGCATTTAATGCTGCTTCCATACCACTAATAATTGTATATAATCCTTCACCACCTGAGTCAGTTACGCCAACTTCACGTAAGATCTTTAATTTGTTAGGAGTATTGTCACATGCTTTACGAGCTGCATTTTTAGCTAATTTGAAAAATTCTTCTAGTGAAGTTTTATTATTTACTTCTTTTTCTAATGCTTCTGTTGTTTCTCTAATAACTGTTAAAATGGTACCTTCAACAGGTTTTAAAACTGATGAATAAGCTTTTTTAGTCGCTTGCTTAAATCCTTCTAAAAGTCCTTTAATGTCAACGCTTTCAACTTCTTTAAACGCAATTGCAAAACCTTTAAAAATTTGGCTTAAAATAACACCTGAGTTTCCGCGAGCTCCTAAAAGCATATTTCTTGAAATAGTGTTAGTTACTTCACCTAAGTGATTTGAGTTATTTAAAGCCAAAGCATCAGCAGCAGCTTGAGCTGTTGATGACATGTTAGTTCCTGTGTCGCCATCTGGAACAGGAAAAACATTTAAAGCGTCGATCTTATTTTTAGAGTTAATAATGTTATTAGCTCCGGAAATAAAAAGTTGACAAAAAAGCTTACCGTCTAAAATCTTAGTCATTTGCAAGACCTCCTATAAATACATTTAATTTGCCTAAATGTTCCTTTTTCTTATTGAGTTCGTATCTTAAGTGAGAACTAATTGAGTTAACAACATTTTTTGCATTAGCATATTTTAAAATAATAATTGTAGCGCTGAAATGCCATTCATTGTTTATATTTTGAATACTTAAATATTCTTGGCCTAAATTAGGACATTGTTCTGCATTTTCACAGATTCCATAAACCCCCGGCGTTGATTCAAGAATATTCAAAAAAACTTTTCTAATTTCTTCTAAAGTCATTCTTACTCCTTGTGATAATTAAAAGATTATTTATAATCAAGTAAAACTATTTTAACAAAAAAGAAAAAATACAATAAATAAATATTAAAATTAATATTAAATATAGTAATTAAATTAATTTATTAGGAGAATTATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGAACCAAAGAATTAAAATCAATTTATTACATGTATAATGATTTAAAAATTTTCTTGCAAATTTGTATTTCAAAAGTAAATCAAACTATTTTTTATGAGCTAAATAAATACCTTGAAGAAAATGGAATTTATCTAAATTAAACTTTTAAATTTTAATGAAAACAACTATAAAACAGGACTTTTGGTAAAAATCTTGTTTTATATTTTTTAATTAATTTAATTTTTTGTAATAATAAAAACATTTTTTAATATAATTAAAATAGTTATTTTTTAAAATGTAATTTTCTGCAAAAAATAACTTGAATTTTAGGAGTTAAAATCGCATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGAAAAAATAAAAATATTGAAGGCGATTATGTCAATGCCAGTGCTAATTACTTAAGTAAAAGAGTTAGCGGCCGTGACAAAACTATTATTAATATTGCAAAAATTAAAAAAATGTAAATAAAGTATTAAAATAATAAAGATTAATTTACATTCACAAACATATATTAAATAAATAAAGGAGAAAAATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGAAAAAAATTAGGAATTGGAAATATCACAACTTTAGAAGGTAATTATCGTTACAAAGATCAAAAAGCTTACATGACAAGAATTCAAGAAGTTTCAAATGCAAAAGATACTCTTGCAGGTCACTGAGAAATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGAATAGGTTTAGACAACTTATATCGTTATGGCAAAGCAGCAAGACAAATTTGTTCAAGCAAACCAGAATGAAATGTTGGACGTATTATTGTTAGACCATTTATTGGTGAAAAAGGAAAATACACAAGAACATTCAACCGACATGACTATGCAAACCAACCTCGTCCAATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACTTGACTCAAAATTAGGTAAATTAATTAATGCTATGAAAGAAGACGACTTATTAATTATAACAAGTGACCACGGTAATGATCCTCTTTATCCAGGATTCAACCACACTCGTGAATTACTTCCTTTAACAATCTTTTCAAAAAGATTCAAAAAACCTAAAGTTCTTGAAGATGTTCTTGGTTTAGGAACAAGTGGAAATATTGTTGCCCGTAACTGAGGTGTTAAAACTATTGAAGAAACAGGTGACGATATCTTCGATCAATTAGTTTAATATTAAAAATAATACTTGCTTTTGGGCAAGTTTTTATTTTGCTTTTTTGCAATAAATAAAATCAATAATATACTAAAATATTTATATATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTAAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGATTAACAAAAGCGAAAAGTGTAGTTGAAAAATACAAAAAAGGAAATAAATAATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATTAATTAATTCATTTCTTTTTTAAGGATATTGAATTTAAACAAACCGATGATCAATTAGCTTTATATCTTGATGAAAGAATTAATTTTTTAGAAGATCTTTTTTCAAGAACTAGACGATGATATGAAATAATCAAAAACAAAATCAAGAAATTAGAAAAATAAGTTAAGAAATAATACTTATTTCATCTAATATTGCATATAATTAAAAAGTATGAATTTTATTTATGGCTCAGAAAATTTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAATTTTTTGTACAATAGCACCTATTTAATTTATATTCAAATATAAAATAAAGTAAATGAAAACAAAAGAAAAATTATCAAAAAATAATATCTCAAGGAATTACATAAAAAATTAATTGAAAATAATTTTATTTCTAAATTATAGAGAGGAATTTGCATCAATCCACTAGAAGTTATTGACCAATATTATTTTTATGAACTCAAATATAAAGAACATTGTTTTTTTAGAAAATAGTGCAATGTATTTGCAAAACATAAATGATACTTTATATTACAAAATCGAGGCAAATTTTAAGCATGACTATAATACTTCAAAAGTAAATAAAGATATTAAGGCTCATCGCTTAGTTGAATGAAAACTTAATTTAGGAAAAGAAAAAGTTAAAACCATTTTTGGAAATTATGTAACAACATATAATAAAGAAAGAATTATTTGCGATTTAGTTGAAAGTATTGATGATTACGATCTTGAAACAAGACCGTAACTTTTAAAACATCTAAAATTTAAATCAAAATTATAAAAGATTATTTGATTATGGTAAAAAAATGGGTATTATAAAAAAATTATGAAAGTTTGAAAAAAATTAAAAAACAGTTTAAAAGAATATGGCAATCAATAAAGATAAGCGAAAATATTCAATTGTTGAATTTAAAAATGAGGGTTCTAAACTAAGGGACCTCATTTTTGCAACTTGAATTTGAAGTTTAAACAAAATTAACAAAAAAATGATAAAAATTGATATAATAAATCAAGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAATGCTAAAACACTTGCGCGAGCAGGCGTTTTTTATTTTATTTTTATACAACAACTGTGCCTAATTAAATATATAATTGAATAAAAATATTAATATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAATTGTAAAAATTGATAGGGGAATATATGTAGATATTGATACTGCAGATGATGAACATTATGTATTTCAATTGAAATACAAAAAGTGCATTTTTTTTGAATTAGTGCAATGTTTTTGCAAAAAATTACCAATGTTTTATATCATAGAACAGAAGCAAATTTTAGTACAAATTACAATACATCAAAAATTGATAAGAAAATTAAATGCCATAGAATTATAGATTGAAAACTAAATTTAGGAGTTGAAAAAGTTAAAATAATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAATATTGTATTTACTTGAATAACTATAAAACAGCTAAATTAGAAAAATTTTAAACTATCCAGTTTGTTAAATTATTATTAAAAAAGTAATTTTAATGTATCATTTAATAAGTATTTTAAAAATATAATTTTTAAATAAACAAAGGAGAATATTATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGAGTGTTTCATGATGGAATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAAGCCTATTTTATAGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCATATCAAATTTTGATCAAGCAAGCTATAAGCTTGTTTTTTATTTAACTACTTGATTTTGAAAATTAAAAAATAATAATTTGAACAATTTAACTTAAATTTTAATATAATTGATTTATGCAAAGTAATAATTGACAAGCAAAAAATGGTGCTAATAATCAACAAGCACAAGATAATTTTAAAAACAAAGAAAAACCAAAGCCAAAAGAAAAAATTAAATTTAGAGGAACTAAATTTAATTTTAAAAAAGTTTTAATAAGTATTATTGTCTTCATTTTATTTTTTTTGATTAGTTTAAGTTCATTGATTACTTTCTATGTATTTAAAAATGAAACGATGCAAATAACATCACTTTTCTTATTATCTCTATTTGGTTTTATATTGTTAGTTTTTTGAATTATTCTTTTAATAGAATTATCAACAAAGAAATGATGATATTATTTCACTCTAACAATGTTTGGACCTTTTACCTTATTTATTATTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGAAAAAGAGATACAACTACTTTTAATGTAGAAGAAAAAATACAACAAAATAATTCACAAAAAAATTCTGAAGAAGAAGCAATAATTGTTACTAACTTCCTTGAAGATAACGAAGATTAAAAGCTGCACTTAATCAGTTTTTTTATCGCATTAAAAAACCTAGACAAATCAAGTTGTCTAGGTTTTTTAAGCATGAACTAAATTGATTTTATTAGCTTGTTTGCTTTAATGAAAATAATTATTTTCTTAATTTCATTTGCAATAGTTAAACCTAAAGCTAAAGGTAATGACATTCAAATTAAGATACCATTGCTTGCATAAATGCATTCATACATTCTAAATACTTTAGCTACTTGAGGCACTAATGCAACAAATAAAGTTGAGAAAGTTGAGAAGCTAGCAGCTAAAACAACTGGTCAGTATTTTTTAATATTGCTAATAAAAATACTTTTATCACTCATTAAATTAATACTATTTAATGAAGCTGCAACACACATTGTAATAAATGCTGTTGTTGAAGCGATTTTAACTAAATTAGCTGGATCATTTTTAATAGCTCACTCAGCTCCAAGTGCATAAGAAATAAGTGATAATAAACTTACTAAAGCACTTTGTCAAAGTAATTTAATTCCCATTCCTCGAGCAAAAATACTTTCGCTTTTTGAAAATGGTTTACGTTTCATAACATCTTTTCCACTATCAACAAAACCAAGTGCAATAGCTGGAAGTCCATGAGTTAATAAATTAATTCATAATAATTGGCTAGCTCCAAAAATGTAAAATCCATCTGGATAATAATCTTTATATGCTAAGAAGAAAGCGACCATACCAATAAGCATTACTAAAATTTCTGTTAGTGAGCTAATTAATAAGTTAAGTATCACTGTTTTGATTTTGTCAAAAATTAAACGACCATTTCTTACTGAATTAACAATAGTATTGAAGTTATCGTCAACTAAGATTAAATCAGCTGCTTGTTTTGAAACTTCAGTTCCTGTAATTCCCATAGCACAACCAATGTCACTAGTTTTAAGAGCGGGAGCATCATTAACACCATCACCAGTCATAGCAACTACTTTTTGGTGTGATTGTCATGCTTGAACAATTCTTAATTTGTCTTCAGGATTAACACGAGCATAAACTGAAATATGTTCAACTTTAGCTCTTAATTCTTCTTCACTCATTTTGGCTAAATCTTTGCCTTCAAGACAAGTATCGCTTTCTTGATAAATGCCTAAATTTTGAGCTATAGCTCTTGCTGTAGTTACTTGATCACCTGTGATCATAACAACTTTAATACCAGCACCTTGAGCTTCTCTAATTGAATTTGCAACCTCTGCCCGAGGTGGATCAATCATAGCAACAAGGCCAACAAATTCTAGATCATTTTCGTCCTTAAAGTCTATTTTACTGTGCTTTACGACTTTTTTAGCAACTGCTAAAACACGATAAGATTTGTCAGCTCATTGGTGATTAATTTCTTTAATATCATCAATAGTTAAATTATTACATCTTTTTAATAAAACATCAGGAGCACCTTTAGTAACTAAAAGCATTCTATCTTTTTCTTTGAATACTACAGAGTGCATTTTACGATTTGAATCGAAAGGCAAAGATGCTAAAATTGGATATTCTTTAAGTAATTCTTCTTTTTTATAACCATAAGAATAAGAGTATCTTAAAAGGCCTGTTTCAGTGGGATCACCTACTTCTTTTCATTCATTATTAGCTTCTAAAGTAATAAAAGCATCGTTACATAAAGCTAAATATTTAGCAAGCGGCCTAAATAATTCTTCTTTCTTGTTTGCACTTCAAAGAGTTCTAAAACGATAAGCATCAACTACTGTCATTTTATTTTCTGTTAGTGTTCCTGTTTTATCACTACAAATAATTGAAGCAGAACCTAGGGTTTCAACTGCTAAAAGGTTTTTAACCAACCCTTTTTGTTTGGACATCTGACTAACACCAATTGATAATAGAACAGTGGTAAAAGTAATAAGGCCTTCTGGAATAGCAGCAACTGCAAGTGAGATTCCAGTGACTAAAGCATTAGTATAAACTTGTGAATTTGTTCAATTTCCTGAGTGAACATTATTTAAAATAATTTGTAAAATAAAGCTTACGAATAAAAGGATAATTCCACCATAACCAAATCATTTGCTTAATTTATTAAGTTTGATTTGTAGTGGTGTTGCATTTTTATTTTGTTTTTGAATCATTGAATTAATTTTGCCAATTTCAGTTTCACTAGCTGTATGTTCAACTACAAAAGTTGCCCGTCCATTAATTACATAAGTGCCTGAGTAAACTAAGTGTTTGTTGTTAGCTAAGATTTTGTCATCAGTTGCATTTAAATTTGCGACTTTACTTACTGGTAAACTTTCACCAGTTAATGAAGCTTCTACAACTTCTAAGTTTGAAGCTTCAATTAAGCGACCATCAGCATTAATTGTATCTCCTGCAGCTAAAACAACTAAGTCACCAGGCACTAATAAATTAGCGGGAATAATTTGGACTTTGCCATCCCTAATTACTGTAGCATTGACCTGATTAACTTTTTCAAGGGCTCGCACCGCTTGATCACTTTTGACCTCTTGATAAGCTCCTAACATACTATTTAAAATAATAACTAGCATAATAATTCCAGGTTCAACATAAGAGATAATTAAATCGGTTGTGCTTTTTCCGCCTTGAAAATGTTCAACAATTGCCAAAGTTAAACTAATTGCTGCCGCTATGATTAAAAGAATAACCATTGGATCAACAAATTGTCTAAAGAAAGCAACTATTGGATTTAGTTTTTTCTTTTTAATTAAAGCGTTTTCACCATACTTTGCAAGGTTTGCTTCAACTTCTTTTGAAGTCAAGCCTTGATAATTGGAATAGTCTTTTTTGTTCATCTTTTCTCCTTAAATAAACAATGTTTTTATTATATATTAATTAATATAATATAGTTTAAAAAACTTGTTAGTTGCTATTAAAAAATATAAAAAACACTAATTCTTAAAATTAATGTTCTTCTTATCTTACTTTTTAAGTTTTTATTGTTTTCTCATTTCTTTGTGTATCTAGCTCTTCTATTTGCTTTTAATTCGTTAAATCTTATAATTATATCTCGAGTTCTCTCAACTATTTCATTTCAACGTCTTTCATTTTCTTTAGGATCTTCATTCAATTTTGGAAAGAAGCAACAATCTTCATCATGCTTTACTTCTTCTTTATTGTTTTCATTAACTTTGCCATTTTTGATTTTTTTTACATTTTACATATTAATACTTTCCTTTTAAATTATTCATAATAATTTTATTTAAATCTTAAAAAAATAAAAAAATGGCGGAACAGAGAGGATTCGAACCTCCGCGGGTGTTGCCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCGACTTGAGTACTGTTCCACAAGTGCTTTTTATTATATAACAAAAGTTTAATAAATGAAAATAAAAAGCAGGCTAATTGCCTACTTGTCTTTAAAGAATGTCATTAGGATCTAAAATTAAATCAAAATATATAGGGGAGTGATCACTAATTGCATGAAAGATATATTTTAAATCTTTATCGTAAGTTTTTTTTCTAATAGTATCAACATATGTTTTTCATTCTTCTATTGTTTGATAATCTTTGAGAATATTATCTTTAAAAATATCGTATAAAGGAAAGAAGTTTGCGTTTTCATATTTTAAATTGGTTCTAGTAAAAATCTTGTCATAAGGTTCAGAATATTTTCCCCATGTACTATTACTCAATGAACTCTTGTGTTGTTTTGAGTCAGTTAACAGTGCTTTATAATATTGATCTTTTAGTATTTCACTAAAAGCAGTTGCTTGATTTCCTAGTTTTATGTTGGTATCGCCCACAAAAATAAGATCATCATTGTCACCATCTTGTTGATCAAATCAATCCATAAGGTTCTTTAAGTTGTAAGCTTCATCAGCTTCTTGAGCGCCTTGCTTACTTAATAAACTTGATTTTGATTCGCCACTTTTTGCTGCTCCTGGACTATCTAAATGATCTATAACAAAAGTGAAATTATTTTGAATTGGTCCTAAAGTTTTAAATTTAACTCCCATTGGAGGTCTAACATAACCAAGATTATTGTTTTCTTCATTAACAACAAAAGTGCTATTGTCATAACCTAATCATTCCTTGCCATTAATAAATTTTTCAATAGCAATCTTATCTTTTTTATAAATAAAGGCTGGACATTCATGTTGTTTTGAAGTTGGGGCTAATTTAGGATTGTGAATAGTATTATCAGAAGCTATTAATTCTCAATTGTTGTTTCCCGTAAATTTTTTTAAGTATTCACAAAATTTTTGTGCTGAAGCTTTTGTTTTATTTTCAATAAGCCCAATAACATCAAGATTTAAATGTTCAATAACACTAGCTAAACCATATTTTTTGTATTCTTCAGATGTTTCATATAAATTTAAAATATTCCACATTCCGATACGAATTTTAGATTTAGCTACTTTAAAACCAGTAATTTCAAATACCTGAATATCACTAAAAGTATTGTCTTTATTATTTATTTGAAAATGAACTTTGACTGAAGTTGGTAAGCACTCTAATTTGTTATAAGTAATGTTGTATTTTGTTGAGTCAATGTTTGCTACTTTTAGTTGAGTATAATCAATGACACTATTACTAAAAATGTTTTGTTTATTATTAACATCAAAAACAATTTTTTTAGCTATTTCATTTAATTCTTCAACTGTTTCGCCACTAATATTTTTGCTAACTTTAAAGCCTGTTAATATATATGTAAACTCATTACTTTCTAAGTTATAATTTTTATTTTTTAATGTAAAAGTTACTTTAATTTCATTGTTAGTTTTAGCTCAATTTTTAAAAATAACTTCTTCTGTGTTTTTATCAAAACCACTATAAAACAAGTCTTTTAGTAAAATTTCAGAGGTTAATTTTTCAGCTATTTTTTCAACTTCAACCTTAACATTTTTAACGGATTTATTAAGCTTATCAACAATTTCTTGTTTGTTAATTTTATTAGCTTTAAAACCTGTGAAAGTGATTTTAAAACTTTTACTTTTTAGATTATTTTTTTCATCTTTTAATACAAAATTAACAGTTATTGTACCTTCTTGATCATCTTTTTTTATGCTTTTAACTTCAACTAAATATGTTGCGCAATTGTAAAATTTTAATTCATTTTTAATCTCTTCATCATTTAAATTTTTAACATCAATTTGAGCTTTTTTACTTGCTTCAAGATTATCTTTATCTCATCAAATTTTGATTTTTTCAACCGCATTATTTAAGTCATTTTGAATTTCATCTTTGTTTTTTTCTTGTGTTTTATTCATTTCAAAAAAGCTACATTGAGTTGATAAAAAAGGTAAGGATATTAAAGAAAAAGACCCTACCAATAATTTAATAAATTGTTTTTTCTTCATAATATAAACACCACATTCTTATATATAAAATTATATTAAAAAAATTAAAATTGTTTAAATTTTTATTTAGTTGACTATTTTCATATTATTTCCAAAAGTGGGGGTGGTAAGTTTAAAGAAAAATGCATTTTCTTAAATTGACTGCTTAAATTTGATTTATTCTAAAAGTCATCATTATGAATTTTGTGTTTGAATCATCTTTTGTCATAATGTTATTTGTCTCAATATTTTCTTCTTTTTATTTGTATAAAAAGCTGAAAAAATAGAATTTGTAGAACAACTATTCCACCAATTAATAAAGATATTTTTTTCATATTTTTTATTTTGACATTTCTTTTTTGCAAATACATGATTATTTGAATAACTAGTATATAAAATATTTTTTGCTTTATTCAAATGCGAAATAAACAAAAAATAAAAAAGATAAACAACTAAAGAAAATTGTTTTTTAAGAATTATTTAATTCTTTTTTTCTTTTATTGTTTAAACTTTTTATAATTTATTAATTTGTGAAAAAATTAAAATTCTTTAACTTTTTCATTAATAAAACTTTTAATCTTGTCTGCAATTTCAGGTTCTTCTAAAGCATAATCAATGTTTGCTTTAACAAAGCCTTCCATTGATCCAAGATCATATCTGATGCCTTCAAATTCATAGGCATAAATTTTTTGTTTATATTTTTTCATTAATTCATCAAATGCATCAACTGCTTGAATTTCATTTTTGCCATCATATTCAATTCTAGATAAGATATCTAAAATTTCTGGATTAAAGACATAACGTCCAAGTATAGCTTTATTTGATGGTGCTTTATCAGCCTTAGGTTTTTCAACTGCACCATTAATTTCAAAATATTTCTTATCTTTATCTTTCAAGTTTATTGGATTAACAATTCCATATTTATGAATATATTCTTTTGCTACTGATTGTACACCAATAACATTTGCTCCAGTTTTATTATAAAACTCGATTAATTGCTTAATAGCAGGAGTTTTTGATTTAATTAAGTCATCACCTAAAATAATAGCAAACGGCTCATTGTCAATATATTCTTTACAACTTGCAAGTGCATGACCTAAACCAAATTGTTCCTTTTGAATTACTCTAGTAATACATGAAGAGCGATTTGTTCTTTTAACTTTTTCTAAAATATCTTTTTTACCTTTGATAATTAATTCTGTTTGTAAATCTTGATCAACTGAGAAAAATTTTAATAAATCCTTTTTCCTTTCACTTAAAACAATTAAAATTTCCTCAATACCTGAATCAAGTGCTTCATTAACTAATCTATCAATAACAGGTGTATCAAGAATAGGCACTAATTCTTTATGAATAATTTTTGTCATAGGTAAAAATCTTGTTCCTCAGCCAGCTGCAGGAATAATCAATTTTTTAATTTTTTTAATTTCATTCTCTTTTGTTGTCATGTTCCTAATTATACTATATTGTTTTTATTTTGGAATGCTTCTCTAGCAATGTAAAAATTTGAAAAAAATAAAAAATCAGTCTAATGACTGACTAAATATTTTGGTGCTTAAACATATGGCGATCTTAAAGGGACTCGAACCCTTAACCTTTGGGGCCGAAACCCAACACTCTGTCCATTGAGCTATAAGATCAAGTTAATTAACTAACTCTATTATAAGTGATTATTTAATATATGTCTCAATTTAATTTTTTCTTTAAAAATTTTACTATCTCGCAGTCTACAGCTTCTATTGCATTAATTGTACTGAGTTGCCTAGTTGCTTCGTTACAACTAATTTTGCAACTAAAAATATAAAATCATCTGAAAAACTTTGTAATCTTTTGGCTTCATTATTAACTAAATAGTGTCTCAGTTTCTTCTATTATTTAATCATGAACCTTTACCACCTACTCTTAATGTATAACCTATTTCTTTATTTACTTCTGCATTGTCAAAAACTCAATTAAGTTTTTTGGTTAATGGTATTGGTTTTGGAAAAGTGAAATTATATTTAAAATCTTTTCTTATTCCAAGCATAAATAATCTTGGTCTATGCTGTTGCAAATTAAAATCAAAAGCTTTAACTATTTAAAAATGAAAATTGTAATCTAATTCATTCAATGTATTTTGGATAATTTAAAAACTTTTACCATTATTATAATTTTTTAATTCTCTAACATTTTCTAGAAAAATAATTTTAGGTTGCTTTACTTTTATTATTTCAGCTATATTAAAAAATAAAGTACATCTTGTATCTCCAAAACCTTTTTTATAGCCTATTTAGGAAAAAGGCTAACATGTAAAACTTGCACAAAGTACATTATGATCAGATATATTTTTGGATTTATTAAAGTTATATCACCTGCAAAAAAATTAGTTGGTTCATTATTTTCATTGAATAAATTTTTGCTATTTTTAAAATTTGTCGCATATGCTTTTCTTGCATTTTTATCTCATTCTGATGCGAAAACGCGTTGACCACCTAAATTGTTCATAGCAATATGAAATCCACCTATTCCATCAAATATGTTAATAAATTTAAAGTTTAATTGTTCCTTTTTATTAATCGTTATTAATAATTTAACATTATTTTTTTATAAATAAAATCAGTTTTATCATAAAAAGGAATAAAAAAACTTCGTCAAGACGAAGTACATTAAATAATGATATGGTGGCTCCGGCAGGAATCGAACCAGCGACACACGGAGCTTCAGTCCGTTGCTCTACCAACTGAGCTACAGAGCCATAATGGCGGTCCAGACGGGAATTGAACCCGCGATCTTCTCCGTGACAGGGAGACGTATTAAACCACTTTACCACTGGACCAATTGGTGGCGGAGGCTGGACTCGAACCAGCGGCCTCGAGGTTATGAGCCTCGCGAGATACCAAACTTCTCCACTCCGCGATGTGATATTTTTTTATTTAGGTGTTCATAAAGAACAAATGGCGGGTGATGAGGGATTCGAACCCCCGCGGGCCGTGAAGCCCCTGCTAGTTTTCAAGACTAGTCCCTTCAGCCAGGCTTGGGTAATCACCCTTGGTGGACCCAACTGGATTCGAACCAGTAACCAACCGGTTATGAGCCGGTTGCTCTGCCGTTGAGCTATAGGTCCAAAGCAGTTAGGATTTTCTCCATATAATGGTAGCGTCGATGAGAGTCGAACTCATGACCTTCCGGGTATGAACCGGATGCTCTAACCAACTGAGCTACAACGCCATAAATGGTGGAGGATATGGGATTCGAACCCACCACCCCTTGCGTGCAAGGCAAGTGCTCTAGCCAAATGAGCTAATCCCCCACATGGTGAAGAAGACAGGATTTGAACCTGCGACCACTACGTCCCAAACGTAGCGCTCTGCCAAGCTGAGCTACTTCTCCTACTTTCAATGCTTCACTTTCTAAATCAATAAGATAATAAAAAGCATATATATTATATACTAATTTGTCTTTTTGTAATAGAATAATTTATTTTTTTTATAATAATTTTTTTTAACTTATAAAGCTTATTTATAGTGTTTTTAGTCAAATTGTTAATTGTTTTCAAAAAAGAATTTTATTTTTATTGCTTAAGAAATTAAAATAGTCATTTAAATAAATTGCTTATTTTTCACAAAAATTCCATTAAGCTTTTGAATATCTAAATGTTTGCTAAAATATATATAATAATTTGATAAGGATGAACAATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTTGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGATATAAATTGAGGATAAAAAAGGATTGATTTGATCTTTCAATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGATGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATCAAGTGGCTTGATTAAAAGAATTTGTAGAGCAATTCAAAGACTAATTAAAATAGTATTAAAACTATTTTAATTTTTTTACTTTTTTAATTGTTTTATAGGCTTTTTGGTAAAAAAATGAAATAAAAAATGTCACACTTGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAGCTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAAGTCTCACAGTCAAGCACACTTCTACCTTTGCGCTCTACATACGGTTTCTGACCGTACTGAGTGTACCTTTGAACGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCGTCACGCACTGTCCACCCACCTGATGATGATGGCATGTTAGAAACTCAATATACCAAGGGTGGTATTTCAAGGATGACTCCACGAGAACTAGCGTCCTCGCTTCAAAGTCTCCCACCTATCCTACACATGTTAGACCAAGTTCCAATACGAAACTGTAGTAAAGCTCCATGGGGTCTTTTCGTCTTGATGCGGGTACCCAGCGTTTTCACTGGGACCATAATTTCACCGAGTCTAGTGTTGAGACAGTTGAGAGATCATTGCGCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCATTTCAACGCTTCGCAAATGCTAACGCATCCACTTAACCTTCGGGCACTGGGCAGGCTTCACCCCCTATACATCACCTTGCGGTTTAGCAGAGAGCTGTGTTTTTGATAAACAGTTGCCCCTCATAATTTACTGTGGCCTAAGTGTTACCTTAGGCGCCCCTTCTTGCGAACTTACGGGGTCATTTTGCAGAGTTCCTTAACACTAGTTTTCTCGCTCGCCTTAGAATACTCATCTCGGGGACGTGTGTCCGTTCTCGGTACAGGTTTCCATAATATTAAGTTTAGAAGCTTTTCTTGGAAGTGTGGAATTATCTAATTCGGTTTGACCCTATGCATCACGCCTCCCGGTTATAGACTGCGGATTTGCCTACAGTCACCAGTTAACGCTTACCCCACAATCCAGTAAGTGGTAAGATTATCCTCCTCCGTCACTCCATCACTATTATAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCGTCTTAGGTCCTGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCGTAGATTCTCACTACGAATCGTTACTCATACCGGCATTCTCACTTCCTAGCGCTCCACCAGTCCTCACGGTCTGACTTCATTGCCCTAGGAACGCTCCTCTAACGTAAATATAATTACCCGCGGCTTCGGTATCGTGTTTTAGTCCCGTTAAATTGTTGGCGCAAGGTCTCTTGACTAGTGAGCTATTACGCACTCTTTAAAAGGTGGCTGCTTCTGAGCCAACTTCCTAGTTGTTTATGAAACCTCACAACCTTTCTCACTTAACACGATTTTGGGACCTTAGCCGGCGGTCTGGGTTGTTGCCCTCGCGAGCCGGGACGTTAGCACCCCGGTTCCGACTGCATAGCAATACATAGCGGTATTCGGAGTTTGATTATAGTCAGTACCCCTAGGCGAGGCCATTCCATATTCAGTGCTCTACCACCACTACTTAACACTACACGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCACTATTCACAAGTCATCCGGGCACTTTTCAGCGTACTACGGTTCGGCCCTCCGCTTGGGGTTAGCCAAGTTTCAGCCTGCTCATGAATAGATCACATGGTTTCGGGTATATAACAACATACTAAGACGCCCTATTAAGACTCGATTTCTCTACGGCTCCGCTTTTTTCTGCTTAACCTCGCATACTGTCATAACTCGCCGGTCCATACTGCAAGATGTACGCCATCACCCATAAACGGGCTCTGACTAATTGTAAGTAAGTGGTTTCAGAATCTATTTCACTCCCCTCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGGGTTCCACGTGCCCCGCCCTACTCAGGATACGATCAGAAGACTTAACAATTTCACATACGGGGGTATCACCCTCTATGCCGCTTCTTCCCAAAAGCTTCTGCTATCATTAAGTTTTGTAACTTCATGTAGATCGTCCTACAACCCCCATTGCTGGGTTTGGGCTCTTTCTCGTTCGCTCGCCGCTACTAAAGAAATCATTGTTTATTTTCTCTTCCTCTTGCTACTAAGATGTTTCAGTTCACAAGGTGTCTCACTCAAGTTCCTATGAATTCAGAACAAGGCAACTAGGCATTACCCTAGTTAGGTTTCCCCATTCGGAAATCCCCGTTTCATAGCATATTTCCGGCTCCACGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTTCAGACCCAAGGCATCCACCACAAACTCTTCCTTATTTAAAAGTATTTTCCTATTGTTATAGTTTGTTATGATGTGTATTTTAAGACATTTCAATGAATCATAATTAATATGATTTTCTCGATGAAATCAAAACAAATTAACCATTTAATTTAATTTTGATGTCGTTGTAAATATCAAAAAATTTACTATTCAGTTTTCAAAGAACAAATGAGAGAAAAAAATATTGTTCTCTCAAAACTAGATATAAAGCTTTAGACCCATAAAAAAGCCACATAACAAAAAATATATAAATAAAATAGGTCTAGTGAAATAAGGTTAATAGTACTTTAAAAAGTACTCTTTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACCCCAGTCACCAGTCCTGCCTTAGGCAGTTTGTTTATAAACCGACTTCGGGCATTACCAGCTCCCATGGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGTAGCGTAGCTGATCTACGATTACTAGCGATTCCGACTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGACCGGTTTTTTGAGGTTTGCTCCATGTCACCACTTCGCTTCTCTTTGTACCGGCCATTGTAGCACGTGTGTTGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCGATTACTCGGGCAGTCTCCTTAGAGTCCTCAACTAAATGGTAGTAACTAAGGATAAGGGTTGCGCTCGTTGCAGGACTTAACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCATTCTGTTAACCTCCACTATGTCTCCATAGCTTTGCAGAAGATGTCAAGAGTGGGTAAGGTTCTACGCGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGATCCCCGTCAATTCCTTTAAGTTTTATTCTTGCGAACGTACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGCCGATGAGTTCCCCATCAGCTAATGATCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGTATATGTCCAGTTAGCTGCCTTCGCCATCTTGGTGTTCTTCCTTATATCTACGCATTTCACCGCTTCACAAGGAATTCCGCTAACCTCTACACAACTCTAGCCTGCCAGTATCCAACGCGTTTTGGGGTTGAGCCCCAAAATTTAACGCCAGACTTAACAAACAACCTACAGACGCTTTACGCCCAATAATTCCGGATAACGCTTGCAACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGTTGCTTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTGCGCACTCTATGGTTTATAGATTGCTTTTTAATTATATATAATAAAAAATTAAAGAGAAGAAAATTTTTTTATTTTTTTTCAAAAATTTTGGCAAGTTAATTTTAAGAGCAAAAAAACTAATTTTTTGCCTATAAAATAAGATATTTTGGTTACTTTAATAATTTATTTAGTTAGCTAAATATTGCAAAAAATATGAAATAAAAAAGCAAGGTTACTCCTTACTTCTTTATATTATTAATAGAAATACTTTTTTATAGACTAACAATAGTTAGGTTGCAAACTATAATAAAGTAACTTTTCCACCTTTAGCTTCGATTGCATCAATAGCACTTTTTGAAGCTGCATGAAGATTAACATTGAATTTTTTAGTTACTTGACCATTACCCAATAATTTAACAGGCATTTGTCTTTTAATCAAATTAGCTTTGAATAATGCTTCTAAGTCAATAGTTGCATTTGCTTTAAAGTTGTTTTCTAAATCAACTAAATTAATAATTTGGTATTCGATGTGGTTAACATTGGTAAATCCACGTTTTCCAATACGACGGAATCAAGGTGTTTGACCACCTTCAAATCCTAGTCTGTGACCCTTACGTTTGTTTTGTCCACTTTGACCTTTACCAGCTTGTTTACCTTTTCCAGCAGCATGTCCGCGACCAACACGGTGTTTTTCAACACGTGAGCCAGCGGTAGGTCTTAATGATTGTAATGTTGAGTTCATTTTAGTCCTTTCCTTTATGTAAATAAATTTTTAATTTTACTTAGTCTAAATTAATTAGGCTAAAAGATCTTTAACATCTTTACCACGAATGTCAGCAATTTGTTCTGGTGTTCTTAAAGCTTGAAGAGCTTTTAAAGTTGCTCTAACAATGTTTGCTTTTGAACGTGAACCATAAGTTTTGGTATAAATATCGGTGTAACCTGCAAGTTCAACAACTGCTCTAACTGTACCACTAGCAACAATACCTTTACCTTTTGTAGCAGGTTTAAGCATAACTCTTGAAGCTAAGAATTTAGCATGAACTTCATGAGGTACTGTGATTTTGTTTTGAATTGGAACTGTAATAAGGTGATTTTTAGCATCTTTAACTGCTTTTTTGATTGAGTCAGGAACTTCGTTTGCTTTTCCATGCCCAAAACCAACACGTCCTTTTTTGTCACCAACAACTACAAAAGCTGCAAATGAGAAACGACGTCCACCTTTAACAACTTTAGTTACACGTGAGATATTAACAACTTTTTCGCTGAATTCATTGTCGCTAGCATCAAAGTGATTACGACGGTCACGGCGATTGCCTTTTTCGAATTTACCTTGTGGACGAGAGTTTTGAGAAATTTTTTGACCTTGAGGTCTTTTTTCTCAAATTTGTTTTTTATCTTTGTTTTCAGCTTTAGCTTTAGGAGCTACAACTTCTTTAGTTCCTTGAGCTTGGGCTTCTTTAGGTTGTTCTTTATTAACAACTTTCTTTACTTCTGCTTCTGCCATAATTAGAATTTAACTCCTTTAGCTATACTTCTAACACCTTCTGCGAAGGCTTTAACACGTCCATGATAAATGTAACCTGAACGATCGAATACTATTTCTTTTAATTTAAGGGCTGCAATTTTAGCACCCATTGCTTCACCAGCTTTAGTTGCTGAAGCAATGTTTCCGTGGTATTTAACTTTAGCTTTAGGATCAAGTGTTGAAACACTTGCTAAAGTGTGTCCTGTTGTATCGTCAATTAGTTGTGCATAAAAGTTTGTATGAGATTTGAATACGCAAAGACGTGGTTTAGTAGCAGTACCTGAAATACTTTGACGTTCACGTAAGTGTTTAACTTGACGTGCTTGATTACGAGATAATGTAGCCATAATATTTTTTCCTTTCAACTAAACTACTATTTAGAAGCAGTTTTTCCTTCTTTAATACGAATGTATTCGCCTTTGTATGAAATCCCTTTACCTGAGTATGAGTTAGGACGACGAACATCATGCACAACAGCAGCAAATTGACCAACTTTTTCTTTGTCAATACCACTAATAATGATTTCAAGAGGTTTTGGAACTTCAACTTTAACACCTTCTGGTACACTTAGACTAACTATGTGGCTGCAACCAACAGTTAATTCTAAAGTTTGACCATTAAGTGCAGCTTTGTAACCAACACCTTTGATTGCTAATTCTTTTTTGAAACCTTTTGAAACACCTTCAAGCATGCCTTGAATTAAAGCATTTGTTGTTCCGTGTAATTGTTTTGTGTGTTTTTCTTCATTAGCACGGAGGGTTGTAATTTGATTATTTTCAATTTTAATTGCAATAAGTGGGCTGAATGTTTTTTCTAATTGGCCTAATGAACCTTTAACATTTAAGGTTGTACCATTTAAATTAACTTCAACACCTGCAGGGATATTTAATACTCTATTTCCAACACGAGACATAAAGCACCTCCCTATCAAATGTAGGCAATGATTTCACCACCCACATTTTCCTTACGAGCTTGTTTTTCAGTCATCATTCCTTTTGAAGTTGAAATGATTGCTGTACCATAACCTGATAAAATGGTAGGTAATTTGTCAGCTGAAGTATAAACTTTAAGACCTGGTTTTGAAACACGTTTAAAGTCAACAATTGCACTTTGGTTGTTTTTGTATTTTAATGAAACTTCAAGTACTTTGTCTTTACCTTGGCCTTTTGTAACAAAGTTTGTAATGTAACCTTCATTTTGAAGAATTTCTAGAATTTTAGCTTTTTTATTTGAAAAAGGAATAGCTACAGTTTTGAATTTACGTTGATTAGCGTTTTTGATTCTAACAATCATATCTGAAATTGGATCTGTGATAAACATAATTATCAACTCGCTTTCTTCATGCCAGGAATTTTTCCTTCATGAGCTAAATTACGGAAACAAATACGGCAAATCTTGTATTTTCTTAAAACTGCGTGTGGACGTCCACATAATTCACAACGTGTGTAAGCACGTGTTGAGAATTTAGGATGTTTCTTTTGTTTTGCTTTTAATGAAACTTTAGCCATATTATCTATCTCCTTTAATAAATGGAATGCCGATTAATTCTAATAAACTTCTAGCTTCTTCATCTTTATTTGTGCTTGTGATTAATTGAACATCAAGTCCCTTGATGTGACGAATTTTGTCAAATTCGATTTCTGGGAAAATAATTTCTTCTTTAACACCTAATGAGTAGTTTCCTCTACCATCGAATGCTTTAGGGTTGGCACCACGGAAATCACGAATACGTGGCATTGCAACATTAATTAATTTTTCTAAAAAGTCTCACATACGATCACGACGTAGTGTAACTTTACCACCCATAGGCATGCCTTCACGAAGTTTTCATGAAGCATTTGATTTTTTAGCTCTGGTTTGGAATGGTTTTTGACCTGAAATTTGAGTAAGTTCATTAAGAACTTCTTCGATAGCTTTAGAGTTTGTTACTTCTTTACCTGCTGTCATATTCAAAACAATTTTTTCTAAACGTGGTACTTGCATAACTGATGAATAGTTATATTTTTTCATTAATGCAGGTACAACTGTTTTTGTGTAATAAGCTTTTAAATTCATAATTAAATTTCCTTCTTAGTTTTACGAGCAACTCTAACTTTAGTGTTACCTTTAACTTTAAAACCAACTTTTGAATATTGAGCAGCACCACTTTTGGTAGCTTTTTTAGTAACGATTGCAAGATTTGAAATGTGAATAGGAGCTTCAACGTTCTTGATTGAACCTTCTGTATTACTTTGACTTGGTTTTACGTGTTTAGTTACTACGTTTACATCTTTTACATATGCACGGTTTGTTTTAAAATTTGTTCTAATAATACGACCAATTTTACCTTTATTGCTTCCTGCAATAACAATAACTTCGTCGTTCTTCTTAAGTTTTAATTTCATAATTACCTTCTTTATTTATTATTAAAGAACTTCAGGTGCTAATGAAACGATTTTTAAATAACCACGGTCACGTAATTCACGAGCAACAGGTCCAAACACACGAGTACCTCTTAAAGTACCATCATCTTTAATTAACACAACTGCATTATCGTCGAAACGAATATGTGTACCATTTTCACGGCTAATTCCATAAACACTTCTAACAATAACACCTTTAACTACTTGTCCTTCTTTTACTAAACCATTAGGTAAAGCTTTTTTAACTGAACAGATAATAACGTCACCGATATTAGCTGTTTTCTTTCTACTTCCACCTAAAACTTTGATTACACCAACTTCTTTTGCTCCAGAGTTATCTGCAACATTTAATTTAGAAAGTGCTATAACCATTATTTTTCTCCTTCAACTGCGTGTTGTTTAATTTCAACAAGACGGAAGTGTTTGGTTTTTGAAAGAGGACGTGTTTCCATAATTGTAACGATGTCGTTAACTTTAGCTAAGTTTTTTTCATCATGAACTGCGAAACGTTTAACAACTTTAAAACGTTTTGAGTAAAGACTATGAGCTTTATAACTTTCAACTTCAACGATGATAGTTTTATCACCACGTGCTGAAGCAACACGACCTTGAAGGGTCTTACGTGAACTTCTTTCCATTATTTAGCTCCTCCTTTAGATTCGTTAATTTTTAAAACTGTTAATACTTTAGCAATGTCACGACGTACTTCTCTAATTTTGTGACTGTGATCTAATGTCCCAGTTTTGTTTTGGAAACGATAAATAAATAATTGTGCTTTTAAATCGTCAAGTAATTTACGGAGTTCAGCATTATTTTTAACTTTAATATCTTTATAAAGCATTATTGTTGACCTCCTTGTTCTTTAGCTACTATTCTTCAGCTTACAGGTAGTTTGTGACCACCTAAACGTAAAGCTTCTCTTGCAATTTCTTCGCTAACACCTGATACTTCGAACATCATTGTGTTAATTCTAACAGGTGTATATCATTTTTCAGGACTACCTTTACCAGAACCCATACGAACACCGATAGGTTTTGATGTTTTTGAGAAGTGAGGGAAGATTCTAATGAAAACTTGACCTTCACGACCCATATAACGAGTGATAGCAATACGAGCACTTTCTATTTGACGTGCATCGACTCAAGCTGAGCTTACAGCTTGTAAACCAAAGTCACCATAAGCAACTGTATTACCTTTGAAGGCTTTACGTTTGTCATGACGTACTAAAAATGGTTTTCTATATTTAGTTCTTTTTGGTTGAAGCATCTTTTGACCCTCCTTCCAAAATTTCGCCTTTTGAAATTCACACTTTAACGCCGATTACACCATAAGTAGTGTGAGCATTTGCGTGTGCATAATCAACATCTTGTCTTAATGTGTGAAGTCTCATTTCTCCACGTGAATAGCCTTCTGTTCTAGCCATATCAACACCATTAAGACGACCTGAAACTGCAGTTTTAATACCTTTAGCTCCAGCTTTTAATGCATCATTAATAATTAATTTTTGTGCAATACGAAAGCTTTCACGATTTTCAAGTTTAGTTGCAATAGCTTCAGCAGCTAAACGAGCATTAAGTTCAGGATTTTTAAGTAAAACAACTTGTAAATTAATATCAAGATTTTTGTTTTTTAAGTATTTGTGTAAATCAACATTAATTTTGTCGATTGTACTTCCACCTTCACTAACTAACTTATTTGGTGTAGCTGAGTGAATGTAAACTGTTATTTTATTGGCTTGATTTCTTTTGATTTCAACTTGTCCAATTTGGTATTTACGTACTAATTTATCGAAGAATTTGTAAATTTTAGCATCTTCTACTAAGTATTTTCCATAGTTTGCTTTTTCAGCAAATCAAGATGTATTACGCGCTTTTGTAATTCCGTAACGGAATCCATTTGGATTAACTTTTTGTCCCATAATTTACTACTTTCTTTCTTCTAATGTAATTGATAAATGTGATGTACGTTTAAAAATTGAATAAGCTGTTCCTCTTGAGTGAGGTTGGTATCTTTTAAGTGTTGGTCCTTCATTAACTAAGATTTCTTTAACAAATAATTTAGATGCATCCATTCCGTGGTTGTTTGTAGCATTAGCAATTGCTGAGTTAATAAGTTTAATAAATAATTCTGAAGATTTCTTGTTTGTATTTCTTAAAATACCAAGAGCTTCACGCACGTCTTTAGATCTAACTAAGTCAGCAACTAAACGTGCTTTACGTGCACTAACACGTTGAATTTCAACATGAGCTTTAGCTTGTTGAACAACTGGTGTATTTTGTGCCATAACTATTTCTTACCTTTGTCAGCACCGTGACCTGAGAAGGTTCTTGTAGGTACAAATTCACCTAACTTGTGACCAACCATGTCATCTGTTACATAAACATCAATAAATTGCTTGCCATTGTGTACTTGGAATGTTAAACCAATGAAACTTGGAAAGATGGTTGAACGTCTTGATCAAGTTTTAATTGGTTTTTTTGGCGCTTTGCCTTCAACAATAGCATCTACTTTTTTAAGTAAATGTTCGTCTGCAAAAGGTCCTTTTTTAAGACTACGAGCCATTATTTAGCATCCTTTCTTCTTCTTACGATAAGTTTGTTTGAAGATTTCTTAGTTTTTCTTGTTTTAACTCCAAGAGCTTTTTTACCTCAAGGAGTAAGAGGAGCTTTACGACCAACAGGTTGTTTACCTTCACCACCACCATGTGGGTGATCCACAGGGTTCATAACTGAACCACGAACTGTTGGACGAACACCTTTATAACGGTTTCTACCAGCTTTTCCTAAATCAACCAATAAATGTTCTTCGTTACCAACTGAACCGATAGTTGCACGGCAACGAGCTAAGATACGACGCATTTCACCGCTTTTTAGACGTAAAACAACATATTTACCGTCGTCATCTTTACCTAAGATTTGTGCAGATGTACCTGCTGATCTAGCAATAATACCTCCACCACCTGGTTGCATTTCAATGTTGTGTACAATTGTACCTTCTGGAATGCTTGAAAGTGGTAATGCATTACCTACTAAAATGTCAGCGTGTTCACCTGAAATAACGCTTTGGCCTACTTTAATGCCTTCAGGCGCTAAAATATACCTTTTTTCTCCATCAGCATATGCTAATAAACAAATATTAGCTGAACGGTTAGGATCATATTCAATGGTCTTAACTACTGCTGGAATATTGTCCTTATTACGTTTGAAGTCAATTAATCTGTAAAATCTCTTAACACGACCACCCTTGTGACGTACTGTAATCTTACCTTGGTTATTGCGACCAGCACTATATTTTAATAGTGTAAGTAAAGATTTTTCAGGAGCATGTCCACTTAAGTTTTCTGTGTAATGTAGAGATGACATATTACGACGTCCATTCGTAGTTGGCTTGTAATGTTTAATTGCCATTTCTAATTTTCTCCTTGCTTAAAAATAGATGAGGAAATTTTTCTTTTAAGCAAAATTTAATCCTCTAATAATTATTTTGAACTCTTCTTAGCAGCTGCAACTTTTTTAGCAGCAGGTTTTGAAGTAGTTGATTTTGTAGTTTTTTCAGCTTTTGAGCTGTCTGCTTTTTTGCTTTCAGCTGCTTTAGCTTTTTTAGCAGCAAGTTTTTTAGCAATTTCTGCTTCTTTAGCTGCTGCTTGTTTTTTATCTTCAGCTTTTTTAGCTTTAACTTTAGCTTCTTTTTTAGCATCTTGAGCTTCAGCTTCATTTGGATAATAAACGATTTTATCTCCTTCAGCTAAAGTAATCATTGCTTTTTTGTAACGATTTGTAAAACCGTTGTAACGACCTACTCTTTTAGGTTTTTTGTCAACTTTTAAAATGTTAACTTTAACAACTTTAACTTTGAAAATAAATTCAATTGCTTCTTTAATTTGATATTTGTTTGCAGCTCAATCAACTTCTCATGTGTATGTGTTTGAACTTTGTAAAATGTTGGTTTTTTCAGTGAGAACTGGTCTATGTAATACTTGAGTAATTTCCATTATTTAATTCTCCCTTCAAAGTTTTTAAGACCTTCATTTGAAAGAACTAATACATCTGCTCAAACAAGGTTTTCAACACTTACTGAGTTTGGTTTAACACATAAAACATTTTGTAAGTTAGCTGTTGATTTGTAAACAGTTTCATCTGTTGTTACCACTAAAATGTGTTTTAAGTTTTCAATTTTTAATGATTTAAGTTTGTTAACAGCATCTTTAGTTGAGATTTTTGACATTTTAAAGTCATCAATAACAACTGATTTTTTGTTTGAAAGTAATGTAAGAGCTGATAAGAATGCAGCACGACGTACTTTCTTGTTAACTTTTAATGAGTAGTTACGTTCTGGAGTAGGACCAAATGCACGTCCACCACCAACTCAAATAGGTGATCTTGTTGAACCTGCACGAGCACGTCCTGTTCCTTTTTGTCTTCAAGGTTTTTTACCACCACCGCTTACTTCTGAACGATTTTTAACATCATGAGTACCTTGACGTCTTGATGCTCTATCAGCCATAATGGTGTCAAAAATTGCTTGTTCATAAATTTTTGGTGATGCAAATAATTCTTTTGGTAAGGCTTTATTGTCAAAGTGAAGCAAGATAACTTTTGTAAATTTTTCTTGTTCAAGTTTAGCATCATTTAATTTAAGTGAAGCAACTTTTGCTCTTGAAGATGCATAAGTTTTAGCTTTTCTTTTAGCAGCTTCTGCTGCAGCTTTTAATTCATCTAAAGCTGCTTGTGCTTCTTTTTCTGCTTTTGCTTTTGCAGCAGCTTCTTCTTTAGCTAATTTAGCTTTTTGAGCAGCTGTTTTAGCAGCGGTTTTCTTTTTAGCACTTTCTTCTTTAGCTTCTTGAGCTTTTAATTCAGCTTTAGTAGGCTTTTTAGGTTTTAAGTTTTTAGTTAAATCAATTTCAACTGTAACTTTTTTTCTTGAAGTTGTTTTCTTTTCAGCTGTTGCTTTAGGAGCAGCGGATTTTTTAGTTGTTGTAGTAGCTTTTTTAGCAGTAGTTGTTGATTTTTTAGCTGTAGAAGTTGATTTAGCAGTAGTAGTTTTCTTAGCAGTTGCTACTTTTTTAGTTATAGTTTTCTTTTCTTCACTAGCTTTAGTAGTAGCTTTTTTCTTTGTTTCAGCCATTATTTATCTCCTTCTGCTTTTTTACTTTCTTCAGCTGCTGCAGCTGCATCAGCTTCAGCTTTTTGGATAAGAGGTTCTAATTCACTTGAATGCATACCAACTGTAACTTCAATGTTGTATTTTTTAGCTCTTTCAAGCAATTCATTCATTTTAAGAACTTCTTTAATATCAACAAGTTTAATTGCATCATGATTTGGTAAGCCTTTAACAGCTTCACGAATCATAACATATGAATTTTTAGCCCCAGGAATTGATCCTTTAACTAACATAATGTTGTTTGCTACATCAACTTTAACAATTTCAAGATTTTGAACTGTTGTTCTAACAGCTCCTAAGTGACCTGGCATTGTCATACCTTTGAAAACACGGTTACCTGAGATGTCCCCAAGTGATCCTGTTTGTCTAACAGGTTGACTACCACCACCACCACCGTGTGATTTAGGTCCAATGTGTTGGTTTCATCTTTTGATTGTACCGGCAAAACCTTTACCTTTTGATGTACCAGTGATATCTACTAATTCACCAGCTTTAAAAATGTTGGCCTTAATAGTTTGACCTAATTCATAGCCTTGCATGCCACGAATTTCTTTAACGTAGCGCTTAGGTGTTGTTTTAGCTTGAGCAAATTGACCTTTAAGAGGTTTATTGCTTCTGTGTTCTTTCTTTTCACCAACAGCAATTTGGGTTGCAACATACCCATTTTTGTCAGCTGAAAGAACTTTAGTAACAACATTTGGTTGCACTTCTATAACTGTAACTGGAACACTAGCTCCAAGTTCAGTAAAGATTTGTGTCATACCAATCTTCCGTCCTAAGATTCCTTTCATAATTTTTTCTCCTTAAATTTTGTTTGTAATTTAACAACCGTCGCTTGTTGTCTAGCACAAGTCGGATTTTGTTATTTTGTTGAGATTTGAACTTCAACACCTGCTGGAAGTTCTAATCTCTTAATTTGGTCAAGAGCTTTATCGTTTGGGTTTAATAAAACGATCAAACGTTGAAAAGTTCTTGATTCAAATTGTTCACGAGATTTTTTGTTAACGTGAACTGATCTTAAGATAGTCACAACTTCTCTTTTTGTTGGAAGAGGAACTGGACCACTTACTTTAACTGAGTTTTTGGTAGCAACTTCAACAATTTTCTTTGCTCCAAAATCAACTAAAGCATGATCGAAACTTTTAACCTTGATATTGATTTTTTTCATAGTTGTCTCCTTTGTTCATATACGAACTTTACTCCACATAAATTACTTGAAATGTCCTCAACAACTCTTCTTGGCATTTCAACAACCTTATGCTTCTATGTAATAGTTGCTTTTCAATTATAAATGAAGACTTAAAATTTCAAAGAAAAATATTAAATATTTTATTATTAAGTATAAATGCTAAGTAAATAAAATCAGCTAATAAATTAAAAAGTTAATTTTTACTTATTTTTTAGCTTATAAACTCACTTTTTGGTTTTTTGTGCAAATTTTAAAAATGTTGATTTTTTAGCCAAAACATCAATAAGTTATTAAGTAAAAACTTGAAACACCGAAACAAGTTGTTAAAATTAATTTAAACTACTTTTTAATGCTTTTTGTTATTAAAAAAGTGATTTTTTAAACTATACCACATCTCTATAAAATATAAAGATAAAAATAATTAATATATGTATATAAAGAAAATTTGAATTTTTTTGTTTAGGTGTATAAAAAACAGATATATAATGTAGCTGAAGATTAATCACATTCTATATATAATATTTTGAAAAAAATATTTTTGTTTTATAACAATCTATCATTCTTAAAAATAATTAAAAAAATTAATAATAAAAAGGAAAATAACTATTATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTCTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACTCAGATCAAAATATACCTAACATTACAGTTAGCGCTAGTGAAAAAGGTTTAGGCTACTATCCAAAAACAATACAAAGATTAATTAAAGGCAAAAAAAATAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAATGTTTATCATGATGATATACACAAATTTGATAGACTTGAGAAAGTTTACTTTTGAGATGGTCAATTAGTAATTGATGGTTACATTAAAGATTTAGAAGTTATTAAAAAAGTCAGTTCAGGTGAAGTAGCTCACGAAATTAAAAACAAAAGTTTTGAAGAACAAATTGAAATCATTAAAAAATTATGTAAAGTTAATATTGAATTTCATCCCGATTCTGCTTATGACTACAAAAGTGATGAAGCAACACATTCTCACCCTGATACTTTACACTATGTTTTTGTACGTATTAAAAAAGATAACCCTGCTGATAGTAGCATGGTTTCTCGTGATATCCAAGGCTTTGAGCCTAATGCTAAAGTCGATCCAAAAAAATGACTAGGAGTTTATGCTTCTGGAGGTGTTCAAGTTAAAGAAGGTGAAATTGATCACGTTGTTGGACATAATGCGGCAGGTGACATTGCTGATAAAAAAATAGGTTCATTTGTATTTCGTCCAAGCGTTAAAGATGAAGGCAAAAAAATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGAGTTGAAAAATCAATCAATATTGTTGGCTGAGGAAGCAACTTTGAAATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGAAACAATATTCAATATCGTCATGACTTTGAAAAATTATCAATTGTCAAAGAAAAGACAAAAGCAAATGATGCAGCTAATACACTTACAATAACATTGAAAATTGAAAATATATTTACTAAAGAAACTAAAGAATATCCAATTACAATTACAGGTTTCTGAAATGACGGAAAAGAGTATAAAAAAGGCTTTTTCGACAATAAAGTACTATTAAAAGACTATATTAATGAAGATGAATATGATTTCGATTTAAAAGATAAATTTTTAAGTGGATTATTTAGTTTCGATAAAATTCAAGACCAACTTAAATTCCTAAAAGATAGAAAAATAATTAATGAAGCAAAAGATTTTGATGGGGCTAATGAAGAATTTACAACATTTGCTTCAAAAGATTACAAATACTACTTTGATAAAGAAAAAACAAACAATTTAGAAAAGGTCAACAAAGATAAAAGAACGTTAGAATTAGTATTAGTTAGAGAAAATTTAAAAAATCCTTCAGATAAAAAAGAAGAGATTATTAAAATACATCAATTTTTAAAAGATTCCAAAGATGAAGTAGGTGCATTAATAAGTGCTTCAGGACATATTAAATATAAAAAATATTTCACTCCAACTAAAGACGCCAAAAACAAAAAAGCAAGCGATTTATATAAAGAACTTGAAAAGAACAATTTTGAATTCACAACTAATTTAACAAGTTTAATGTATAAATGAGGTATCTATTTTATATCAAATAATATTAAAAATAGAGATTATGATGACAGAAGTCACTTACATTTAGTTTTAGAAGTAACTGAAAAAGCAACAAAAAAAACCAATGTTATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGAGAAGATACTGCAAATAAAAATAAAACTTCAGGTCAAAAAATGAAACTTTGAGTAGATTACCTAAAAAATAATAAATTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTAAAGAACACTAAAACAAATGAAGAAGAAAATGTTTATATTCTATTTTGCAAATTAGCCTAAAATCCTTTTCTATCGTTTTTCTAATAATTAATGAGTCATAATTGACTCATTTTTTATATCTAAAAAGCAGCAAAAACTGCTACTTTTTGGATTAGATAAATTATTGTCAATCGTCACATTTGTAGGTTTCATCATGGTTGTAATTTTGGTTATTTGATTGTGAATTGTTACCTGTTGTTTGATTTTGATCTATATTAGATTTGCGTTGTTGGTTCACATTTTGATCTTGTGTTTTTCTATTTTTAAGATATTTTTCAAAATAGCATGAAACCACCAATGACTAATAAAATAATTCCTGAAACTAATGTTCCTATTCTTTGAGTACTATCAAATGTTTTTCATTTAACTCAAGCAGCAATAATTCCTACAAAACCACAAATTACACACAAACCACCAAAATTCAAAAACCAAATTTATCAAAAAAATGATTTTAGAGCACTACAGGTTGAATTTTCAACTTCTTGTTTAAGAGCATTTGAAAATGCATCTTTGAAACTTTTTGCTATTATTTGCCCACTTTTCTCTTCTAATTTCAATTGTCATATAATAAAATTATTTAAATATAACCATATTTATTTCACATAAAAATACTCTAATGAGTATAAATGTATTATTATTTATATGAAAAAACATTAAACTATGTTATTTTTGGTAAAAAAGCCTCAATTTTCACAATTTTTTTCAATTTTTTTAGTAATTTAAGCATAAAAGGCAAGTTTTAATTGAAAGGATAAAATCAACATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCAATTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGATTCGCATATCAAGAAAAGAACATTGCGCAGGGTAAAAAAGCGTTAAAAGATATACTTTTAGAAAATCCAGAATTCAAAAAAGAAATCGAAAATCAAATTTTTGGTAACGAAGTCAAAAATGTTTCTAAATAACTTTAAGCCCTATTTTATAGGGTTTTTAGTTTAAATATAAATTATGGAATTTAAAAAAATTTATTGGCGAAGATAACAAAATTAATTTTTGGGTTGAAAAATTTTCTATGTAACATATAAATATTAATAACGAAATCATATGTAAAAAACAAAAGCAAAAAAATAAATAATTATTATTGCAACATCTATTGCATGATAATATGCATTCTTAGTCCCATCAAATCATTCATGGATACAAGCATCTATTTGTAATTCATCACTAAAATGCATGTTTTCTTTTCTGCATAATCTAACTCTTAGTCTTACTTCGCTAAACAAGCCTAAAAAGTTTTTATAATTGTTTCTAACATCTTCTATTTGGACTTTTAACAATTCAGCAATTTTAATCATTTTTTTACTTTTCTTCTCGTTTTCTTATAGTTAAAACTCCTAAGCTTGTTAATCTGTTAACTAAAGTTTGATAACTAATTTATTTTTCATAGCCTTCATGAGAATAATCTCCAACTTTTATTTCTTTTCAAAAAGATTTGATTGTTGCATCATTAACATTTTTGATTCCGTTAGTTCTATTTTTAGAAACAATTTGAATAGCATTGGTTTTTCTATTTAAAAAATCTCTAATATGGCTTTTGTATCTTTGAATAGATATAATTGATAAACCAACTTTTTTAGCAATTTGTCAATTACTCAAATTCATTCAAGGTGCAATTAATTGATAGTTTTTTGTTTGCTTTTTCCAATTTTGCTGGATTAGAACAAAAACTAAATACTAAATCAATAAATACATCCTATTTTACAATTATAAATGTCAAATTGTTTTGTATTGAAGGTATGACAAACTATTTTAGAATTTATATCCCACTTGTTATTTCTTATTACTTTTATATTTTAATAATATAATAATTTTAATAATATATTTTGATTTTTATCAGTAGAATGAGGTCATTAATATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGAGCTAAAGATGGAATTTTTTCAATAATCAATAATCCTGAATTAGTTAGACCTGCTAATATAGATGAAGGATATCCAGGAAATGGATCAATAGTTTATAAATGTAATAATGGAACTACTTTAAGAGTTACTTCATTAATGGGAATTACTTTTAATAAACTATTAGCCCCTTGAACTCAAGAATATGCCAATAACTTTTTTGATACTATAGATAACATCATTGAATATGGAGAAAAAACTGATTTTCACTTTGTTGACTTTCACGCTGAAACAACAAGCGAAAAATATGTTTTAGGTCTTTATTTAGATGGCAAAATTGATGGTATTTGTGGCACTCACACACATGTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAAAAAATGATATAACTAGTTCAAGCTTTACATAGGTAAAGCTGCAAGTTAGTCAAAGAGCTTGGCTATTAGACTAATATATGTGTTACTAATGAACAAGACAAATGTGCTTGAATCAATGGACCAACATTGAGGGTGTAGACTCTTGCATTGTATAGTAAGTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAATCCTTTAATAAAGCTATGGCTTTTATTTTGCATAATGTGATTTTTCACCAACTTTGACATTAAAACAAATTTTGTATTCTCCTGATTTTTCTATTGTTTTATAATCACTTACAAAAAATCTATTTGAATATTTTTCTAAAGCTGTTTGTAATAAAATTATATTAAAAATGCTACTTTTACTTAAAATTAAAAAGTATTCATTAATTTGATCGCTTGATTTTTTAATTTTTCTAAAGCTATTTCTTTAGTTACTTTTGAAGTATCTCACGTCCCTTTCCTCCGAGTCTCCTTTTTTCTTAGTGCCACAACCTTCACTAATTGTTGGTAATAAAATTGTTGAAATTGTAGGTGTTAAAACTGAGAATTTCAATCAAAAATTGTGCTTTTTATTTTTATTTTTCGTTGTACTTTTTTCTATTTTTTTACTTTAAAAATAAATATTATTTTTTTAAAACATTATGTTATATAACGCAAAAGAAAATAAAAAAATATCAGAATTTTAACTAATTAATTCTGATAATTTCTAGTTGCATTTTGTCTTAAACAACTGTTTTACAGTATTTTTTGATTTTATTTAGCTTTTAATTCATTTAACATTTTGTTGTCAAAAGCTTTGACAAATTCTGTAATTAAATCAATTGTTGCTTGAATATCTACTAATGAACAAACGCCTATTGGTGAGTGTAAATAACGTTGAGGTAAACTTAGAGTAATTGTAGGAACCCCACCTTTACCATATTGTAAACTAGCTCCATCAGTACCGCCACCTTCGGCTATATATTTATAAGCTGGAATTTTGTGTTTTTCAGCAATTTTTACTAACATGTTAACTAATTTAGGATCTGTAATAACAGCACCATCTTGTATTAAAAGAGCTACACCTTTACCTAAAACTGGAGTTCCTTTAATACATCCTGTCGTATCATGACTTGCTCCTGTATCAATTGCAAAAGCAACATCTGGTTCAATAATTGAAACTGAAGTTTTAGCACCTCTTGTTCCAATTTCTTCTTGTACTGTACCAACAATATATGTTTCATTCGGTAAATCTAAATCTTTAATATTGTTTGCTAAAAAGTCAATAACTGTAACACCAGCTCTATTATCCATTGCTTTACCAGCAATTAAATTATTAGGCATATCAATTGCTTCACCTGACATATAAATTCTGTCGCCTACTTCAATACCTCATTTTTCAGCTTCTTCTTTTGAATTGAATCCGCAGTCAACAAACATTTCTTTTGTTGTAGGAACTTTAGCACGTTTTTCAGCTTCCATAATATGAATTGATGTATGACCAAAAATACCAAAAATTTCTTTATTGTCTCTATTTGTTACCACACGACATTTTGTCCCAATAACAGCAGCTGGTCAAACACCTCCAACAACTGAAACTAATAAGTTACCATTTGGTTCGATTGATCTAACTAAATAACCAACTTCATCCATGTGTGCAGCAATCATAATTTTTGGTGCATTTTTAACTTTTGTTTTATTTTTAATAATTAAAGAACCAAAACCATCTCTTGAATATTCAAAATTTTTTGAAGCTGTATTCTTCTTTAAAGCTTCAACAACAGGTTCTTCATATCTACTCATAGCTTCAATAGCCATGTATTCTTTTAATCTTTTTGCTAATTTATCATATTTAGCCATTTTTAAACCTCCTTAAAAAATTACTATATGAATTATATAACTAATTTATTTTTGCTAGGTCTATTTTAAGGCGGTAAATTTTTAATTATTTTTTGCATCTTATTTATAGGTCTTTAAGAAAATTAGTTTTAAACTAAAACAGCAAATTTTTATATTTTAGTATCATTTTTAAAATCTTTAATAATTTTTATACATTAAACACAAGCATGAATGCTTGTCGCATTTTTCTTTTTTATAAATTTTTCTAAATATTAGTATACTTATTTCACTTCGGATCGCTAATAATACATAGTATTATAATTTATATACCAAAACGAGAATTGTGAAAAAGAGAAACAAACTAAAGGCTTTAACTATTTTATCAACTGCTTTTGCAACAAGTATTGTTGCTTTTGCTGCTTCATGTAATAATGAAGATAAACAAGTTTATTTTACTTTAAATCATAATTACAACCAAAAACAGCGAAACATATAACATTTTGTCAAATAACTATCGATACTATAATGATTTTGGAATGAAATTAGCTAAAGAAGAAAATTAAGTTTAATATTAAACTAAAGATGCAAACTTTGCATAAAAAAATTAACATCTTTTTATGCAAATTATTAATCACTTTTTTCTAAAATGCCTATAAAACTGAGATTTTAGGCAAAATAGACTATTAATTTTTTACTTTTAGAGTCAATTTTTTGCTTGCAAAAATTATTCTTATTTTATGTAGCAAAAACTATATTATTTTGTTATAATAAATAATATTTATTATTTAAATGGGAGGGAGGTTATTATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGAGATAGCTTCTTCAATCCTTTTTCATTGATCTTGATTTTACTTTCAATAGTTTCATTAATTACTGACATAATACTTCCTTTAACTAAAGGTGAAAAATCCGAACCTTGAACTGTGTCCATTATTTTAACAATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGATTATTAATTAAAATCATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGTGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAAAGTTTTAAAATATGGATTCTTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAATGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGTAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTATGATTTTGCTTGTGGAACAATTCCTTGAGATAAAGTCGATAAAGAACTAATTACTAAACCACGGAAGTGAAATGCTAAAAGCATATTGCGCTTTATGCTTTGGTTTGGACCAGTTTCTTCTATTGTTGATATACTTTCTTTTATTCTTCTTTATTATGTTTTCATTCCTTATGCCCATCCTTCTGTAGCCAAAGATTCAGAACAATTCAAGATGCTATTTTGAACAGGTTGATTCATAATTTCAATGTGAACTCAAAGTTTAGTAATTCACTTCATTAGAACTGAAAAAGCTGCATTTATTCAATCAAAGCCAGCCTTTATATTATTATTCTTTTCAATATTTGGAAGTGTATTAATTACTATTACTCCTTATATTCCTGGCCTTAATACAGCTTTAAAAGTAACACAACTTAATCCTTGATTCTATGGCTTTTTAGTAGGTTCAATTGCTTTATATATTTCACTTGTTTTAATTGTTAAAAAAATCTACATCATAAAATACAATGAGTTACTATAATTTACTAAAACATCTATAAAACAATAGAATTTAAAAAACGGTTACCCGTTTTTTATTTTTGCTTTTAGTTAACTCCAGTAGTAACAAATAATTTTTGAAGAGGTTTACATGGTTTTATAGAAAGCGTTAATAAATAATGCCTTCGCCAACTCCAACTCAATATAAATGAATAACATATCTATTAGAAACATTTGTATGTTTTTCAAAATTAATGGTTAATCATTCTCTGTTATCTTCAAAGAATTGTAGAAATTCTTCTGATGTTCATCTTTGATAAGTTAGTGTAACTAATCTTGAACTAGCAACTTTGTAATTGGATGTTTTTTTCTTCAATATCAAAAACAATGTAATTGTCTTTTTCTAATCCAACTTCCATTAATGGAGTAAGATAATCCTTATAATCAGAATCTGAAAAAACATTTATATTTGCATCTCATTCATTATGATTTGGAACAGGAAAATTGAAAAATATTTTTTTAAAAAATAAGTCCCTCAAGACTTATCAAAAATTAATTTTATGTTTATTAATTGTGTCTGAATATTCTTTAAAATTAGGATAATAAAATTCACCCAATTTATAAAATTCTTCAGAATGATCCATATGAAAGTAGTGGCATAATTCATGAACTATCACATAGTCAATATATTTAGGATCAAGTGCCACTAAAAACAAACTATAACTTAGCTTACTAAAAACATTATATTTTTTGTGGTGTGTACCTCAAGCACTTTTTTTCATTCTAACATAAAAATAAGGGTTATTAATTTGCATTAAAGCACTATAAACAAGCTGTTTTTTAGAAATATAGTCTTCTAAAAATTTAGTTAAAGTTTTTTCGAAAACCTTTAATCTTTTATTTTGATCACTGGCACATTTTATTGAGCATCTAATAGTTAAAATATTGTTTTCTTTATCATAAACCCCATCACTAGTATCGCGATAATGAAAGCTTCATTTTAATAATGTTTCTTTGCCAAAAAAACTAATGTAGTGTTCTTCAACTCCGCTAGTTAAATTTAAAACTATAGCATTATTTAATCTTTTATTTGCTTTTTTAAAAGCTTTAATAAGTAGTCTATCTAAATTATGAAAATTATAATCAGCCTCACTAATATGTCTAAAACTAGTGACCTCAAATACTTTTTGGTCACTATTAAATCTAACGATTATATTTTCACAACGGACATTAACTTTTGAATAAACTGGATAACTAACATCATCAATTATGATGTGTTTAATTAAAACTAAATTATTTTTTCTTTGAGTCATTTTTGTTTAAGATCTTTCCAAAACGTTTAATGTCTTTAAGTGAAGCACTTCTATTTGAACTCACTGATTCATTGATTAACATTAATTCATCTTTATTTAAGTTTCGATCTTCACTCATATCTTTAGCCACATCTTCAAGTAAGTTAGCTACTTGATCTTCAGTTGTTACTAAAGCTCATGATTCTAAGTTAGCTATATCAAAAGTAGTATTTGAAGGCAAGATTACCAATGAAAATACTGGGAAGTTAAAGCCACACATTTTGTAAAAGTGTTGAATGTGTTTAAAGTTTTGAATAAGCGGATTTTTAAATTTCAAAACTTTATTGCTTTTGCTTCTTAACTCTAATTCAGCATTAGTAGCTTCACCACTAAGATGGCCAACATAATATTTAACTTCAATAATAAAAACTGCCTTATTACTAATTAAAATACCATCAAGTTCAAATATTTGACTTTGTCCATATTTAAATAAACCACCATTTAAGTATTTGTAGTTTTTATCTTTAGCAATAGTTAGAAGTTTATTTCTAATTTTTGCTTCAAAACGAAAACCAATTGAATTATTGCGTTTGCTTTTAATAAGTTTGATAATTAAAAAAGTTAACACTAGCACAATAATTAATAGTGTTAAAGGAATTCCAACTGATAGTCCAATTATCATTTCTCTACTTGGTTTAATCATATTTATAATTATATTTAAAATTAGAATTTGTAAAGCAAATTAATAATATAATTAAGAAAAGTGAGTAAGGAGGCATTTATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAACAGCTTTTTGAGAAGATAGTGAAGCAAAAAGCTGAATAACAAAATTAAAGGAAGCAGATTATGTTGTAATTAATAGTCCAATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGATATACTTTTGGAGATCATGTTAAAATGCTTGAAGGAACATTTAACTTTTTAGGAGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATGCTTTAATTTAACTATAAAACAGAGCAATTGGTATAAATTGCTTTTTTATTTGCAATAAAAAATTTCTCGTTTTCATGAGAGAAATTTTTAATCTTGTTTTTTTAAAATAACTTCATTGATAACAAAGAATTTATAAACTTTTTCTAGAATTGCATTAACCATTTTGTAAAAGTTTTTTTCATCTTCAAAATAATATTTAGTTATTTCAACTGATTCATTAATTATTATTTTAGGTTCAATTGACATAAGTTCATGAGCACCATTAATTAAAATGGCTCTAATTAATGGACTAATTCTTTCTCAGCTTCAACCTTCTTTTAAAAATTGACTGACTGCTTTTTTATAAAAATCATAGTTTTTTGCTATCTTTTCAAGATGCGAAAATTGTTCATTACTTAAGTTACTTTCTGAATCAAATAATTCATTGACATTGATTTTTTGATCCATTAATTCATAACGGTAAAGAACATTAATTATACCTAAACGAAATTGACGTCTGTTTTGCATGTTTTTATTTTATAACAAAAGTTATAATTGCAAAATATTTAAATAAAAAGAAGCAACAAGTGCTTCACTAAAATTATTCTAATTCTTCAATCGAAACATTGAATTCACCAATTAAATCATCTTTAGGGCCAAATAATTCATATTTGAATTTTTTAATATTGCCTTCAATAACAACTTCATGTAAAAATGGTTTTAAAAAGAATTGTGTGCCATCTACTTTAACAAAAGAGTTAGGAGCTTTTTGACCTTTTAACATTGTCAACGTTGACATTCCTGTAAAAATATTTACTCTTTCACTATTGATTTCTATACGGTTAGATTCACCAATTGAAGGCTCTTTAAAATGATATGTATGATATTGTAATTGCTTTGGATCTTCATCATCTAAAAATAAATCATATTCTGCATCAGCTTCAAAAGAAACTATTTTAGGTTCTTGGTTTTTAATTTTATGATCTTGAATTGAAGTAAATCTTATTTTCATTTTGCTAACCTTTCTTTAATTAAGTCTTCGCCATATAAATAAATTGCTTTAGCTAATTCAGGACCATGTTCAATGTAAGTGGTTGCTAGACGTATTGGCATAAATAGATTTTTGCCTTTTTTACCTGTAATTTCTTTTGTTTGGTCAATTGCAGCTTGAATATTTTCAATAGTAAAAGTGTTTTTATTTAATAAATCTTTAAAAGTTAAAATTACATCATGATTTTCAGCTTCGACAACTGGTTTTTTATTTGTTGGTTTTAAATATGGCTGCATATCTTCGATTAATTGCGAAGTTGTAAATACACTTTCTTTAAATGTATCAACAAATAATTTGTTTCATTTTGTTGCTTTTAAATTAAGTTCTTTAAGTAACTTAGAAGTTGGTTCTTCTTTTATATATTGTTTAGAAAATCATTGCATCTTTTTGATGTCAAATTTTGAAGGGCTTTTGCTTAGTCTTGCTGGGTCAAATTTTTCAATAATTTCTGCTTTTTTCATAACTTCAACTGTATCTGGTGAACTTCAACCAAGCAAACTTAAAAAGTTAAATACACCTTCAGGATGGTAGCCTTCATTACGATAATCTTCAATAAATTGTTTTAAACTAGTATCACGTTTTGAAAGTTTTTTACCTTCCATATTAGTAATAACAGTTAAGTGGCCAAAGTAAGGAGCTTCTCAATTTAAGGCTTGATAAATTGCTAATTGTTTTGGAGTATTAGTGATGTGTTCTTCGCCACGTAAAACATGGCTTATTTTCATATCGTGGTCATCAACTACAACTGCAAAGTTGTATGTTGGATAGCCATCACTCTTTTGAATAACTCAGTCTCCGATATCATTTGAATTAAAGCTGATGTTACCTCTAACTAAGTCTTCTCATTCATAAGTTACATCCTTAGGCATACTTAAACGAATTGAGTAAGCTTTAGCTTTTTCTCTTTTGGTTTTTTCAGCTTCGCTAATTTTTAATCAATTACGATCATATCTAAATGAAGCAATACCTTTTTTGTTTGATTCTTCATGTTGAGCTTCTAATTCTTCGCTAGTGTCATAAGCTTTATAAGCTAAACCTCTTTTAACTAATTCATCAGCAATTTCTTGGTATCTATCTAATTTTTCGCTTTGGCGATATTTACCACAAGCCTTATTTGGTTTAAGTGGGCTTTCATCTGGAACTATTCCAAGTCAAGCTAAGTTTTCTAATTGACTTCTTTCACCATCAGCTACATTTCTTTTAACATCAGTGTCTTCTAATCTGAAAACAAAATCACCTTTAAAGTGCTTAGCATATAAATAGCAAAATAATGCTGTTCTAGCACCTCCAATGTGTAAGTAACCTGTTGGACTTGGCGCATATCTGGTTCTTATTTTTTTCATTCTAACCTCTTATTTATGACTTAATTTGTATATTAATTAATATTATATAGAAAATGTTTATTTTAGTATCTAAAAGGCAAAAAAAGAAAATTAAAAAAGATATCAAAGTAGAGTGAACCCAAATTCCGAACTTTTTGGAAAGGGGTTCACTCTAAATTTCTAATTAATTTTAATTTCATTGATTTCTCCTTATAAATATAATTTGCTAAATTATTCAATAATTTTAATAAAAAAGCCTACTAATTAATAGTAAGCTTCGATAAATTTTAAGCTTTCGAATTAGAATTTAAAATAGATTTGCAGTCGACAAAACATTGCTAATTACATCTAATGATTCATTCAAATTATCAAAAGGTCTGTTTGGAATAATCGGAGCAAATTTTGATAAATTAGGATTTTTAAATAGTTCGTCTTTATTCTTTATTATTCCATTAAGTCCAAGAAAAATTCCTTTTGCAATTTCATTATTATCATAACCACTGATTTTTTCTTTAATTTTTTCTATTGATAAGTTTTGTTTTTTCAAAGATTCATCAACTTTACTTTTGATTTCAGGATTTTCATTTAATTTATCAATCAATTTGTTAATTGTTTCTTTTATATTATTAACCTTGTTATTTATTTCTTTAACTTTTTTAGGATCATTTAATTCTTCTTTAATATTTTTTGCACTTTGATTATCTTCAGCTTTAAGATAAATATTAATTGCACTTGTCATCTTTTTATATTCATTAGTTAAATAATCATTAAAGTTTCCTGTAGGATTAATTGATTTATAAACTTCAACTATTTTTTCATATTCAGATTCTTTATTAAAATATGTAGCTTTAAGTAATGCAAGACCGTCCTTAGCTTTAAAATCTAAAATTTTTTCAGGTCCATATTCACTTACGTTATTTGCATCAAAATCACTTTGAAGCGCATTTTTAAATGATGCTAATTGGATAATTTTTTCATATGCGTTTTGACAATTTTGAAATTTTTCTTTTTCACTTATTTCATTATTTGGTTTATTGTTTTTAGGCATCGGACTTTCAAATTCAAAGAATTTTGTTGAATTAACATCTTTTTGATTTTTGCCACATGAAACAATAGGAACTAACATGATTGGTGAAGTTAACAATGCTCCAAATTTAAATATTTTTTTTAATTTCATAAAAACCTCCATTTTTAAACACAATTAAATTATATTATTTATTTTACTTTTTTAATTTATCTAATTGTTCTTTTTCTTCTTTAGTTGTTTTTGATAATTCTTTTATTTTTTGTATTGTTTTATTTAAAACATTAATTGAATAATTTCAATTGTTAAGAATTTTTAAAACTTTACTTTTAAAACTCTTGTAAGCTATAGATAAATATCAAGCTTGAACCATTTCTACATAATAATCATTATTGTTAGCTGTATTCATAATTAAATCTAAATAACTTAATTTAGAATCATCTTTTAAATAATATTTCTTAAGCATAACAATAGCAAAACGATTTGTATATGAATTATTATCTCTTAGTCAATCTTTAATATGACTTAAAAAATCTTTTTTATTTTTGTCTTTTAGTTTAAATTCAAAAATATCAACAATAGCTCAATTGTCTAATTTTTTTGCATATTCAACTAAATATTTTATTTGCTTTTTATAATCATTAATTTCTTTAATAAAAAGACCATGAAGTATATTTTCCTCAAACAAATAATGATAGGGGTTTCACATAAATTCTGATACTTTTGTAGGTATTTCTTTATATCATTTTTTAGCTAAATCTTTAAGTATTGGAATTCTAACTCCTAATAATTTTGCACTTTCAATTTTATAAATTTGCAAGCAAAATTCTTTATATTTTTGATCAATATAAGGTATTAATTTATAATAAAGTTTTTCTTCTTTTCTACATCTTAATTCATTCATTTTAATACCTTACTTTTTTTCTTTAAAAATTATACTTTAAATAAAATATTTTTTTGTAATATGTTAAATATTAAACCTATAAAAAATATTGCCAATTTTACTAAAAAGACTATAAAACAATTGAATTAAAAATTATTCTTCAATAAAACTTTAAAAAATATATTTATCATTTAGGGACATAAGTTAAAAATTATGTCCTTTAATTTTTGGGACATAAGGATTAAATTATGTCCCTATTTTTTGGTCAAAAACGTTAGTTTACTTTTATTATTTTCGGGACATAAGAAAAAAATTATGTCCCGAATTCTTATAGGACATAATTTCTATTTCTAATTTATTTCTCAACTTTTTGAGCTTGTTTTACTTTTCTTTTTAAGTAAACTCCATTACCAATTGGAATACCTTTTCATCTTCCTAATTCGCTTGGATCAACTTTTTTTCTTAATGCTCCTAAAATAAATGCGCCAGCAAAAGCACCTACAACAAGAGCTAATAAATATCAATATCATCCTTGTGCAACAACGAATACAAAGATTCCGCCATGAGGAGCAGGAATTGAGCATCCTAATCCAGCTGCCATTCCACCAGCTAATGTACTTGCAAAAATTGATGAACCAATAACTCTCAAGGGATCACTAGCTGCATAAGGAATTGTACCTTCAGTAATAAATGCCATGCCCATTAAGTAGTTAACTTTTGAATCTTTTCTTTCTTTAGCAGTAAATTTTTGAGGGAATAAGTCAGCTGCAATAGCGATTACAAGAGGAGGAACCATACCACCAATCATAACAGCTGCCATAATTCATGGTTGATTGATTCCATCAGGATCTACTGAAGCAACACCAATAGTATATGCAACTTTGTTAACTGGACCACCCATATCAACTGCCATCATACCAGCAAGTAAAGCTCCCATTCCAATTTTAAAGCCAATTGAAATTGTGTTATTTTTAACAACATAGTTTAAACCTTGTTGAATGTAGTAATTTAAATATGAAAGTGGTGTATTAATTACAAAGAACATAATAACAGCAATAAGTAAGATACCAAATACTGGATAAATTAACATTGGTTTAAGTCCATCTAACGATTTAGGTAATCATTTGAAGCATCATTTTAATCCTAATACTAAGAATCCGGCAATGAAACCACCAATTAAGGCTCCTAAGAAGCCTGCATTAGAACCAGTCTCATAAACCGCAAACTGTGTACTAGATGCCATGGCTCCAGCTACCATACCGACTGCTAAGCCTGGTCTTCCTGCAATTGAATGTGCAATAAAACCAGCAAACACTGACATCATAAAGCCAAAAGCAAATCCACCAGCATTTTTAAAGAATTTTGAAAATGGAGTATATTGTCCAAAATCTTTTCCTACTGCTTCTTTTCCTAAAAAAGAGTCAAATAAGAAGGCTAGAGCAATTAGAATACCGCCACCAATAACAAATGGAAGCATATGGCTTACACCGTTCATTAAATTCTTATATATTTGTTTTCCAAAACTTAATTTTTCTTGATTTGTATTTTCATTTGAAGAAGAATCTGATTTATATACAGCTACTTTTTCATCTATTATTTCTTGTATTAATTCTTTGGGTTGTTTAATTCCTTTTGTTACAGTTGTTTGAAGCAATCTTTTACCATTGAATCTTGTGATTTCAATATTTGTATCGGCAGCAACAATGACACCATAAGCTTTTTGAATATCTTCATTTGTCAAAACATTTTTAGCTCCACCTTAACCTTGAGTTTCAACTTTGATTTGATAACCCATTTCCTTAGCTGCATTTTCTAAAGATTCTTGAGCCATGTATGTATGAGCAATACCAGTTGGACAAGCTGTAACAGCTACTATAAATTTTTCACCATTATTAGTTTGTTCAAATTCATTCTGATCAACTTCTTGTTCTTTTGTTTCAATTAATTTTAGAAATTCTTTGTTGCTTTTAGCATTAATTAATTTTTCAATAAATACTTTATCAACTAACATTTTGCTCAATTTAGCCAAAACTTCTAAGTGTAAGTTTTCACTATCTGTTGTTTCTGGAGCTGCAATTAGAAATAGCAATTTAACAGGTTTATTATCTAAAGATTGATAATCAACTCCGTCTTTTAAAACAAGTGCACTTAAGTGAGGTTTCTTAACGAAAGATCCGCGTCCATGAGGCAATGCTAATTCATCGCCCAATCCAGTTGTTGTTTGTTTTTCTCTTTCCAAAACCGCTGTCAAATAGGCGTTTTTGTCAATTATAATGTTATCACCCATCAAATTAACTGCTAATTCAATGGCTTCTAATTTATCTTTTGCTTTTGCATTAATATCAATATTTTTTAGAGTTAGAAGTTCTTTTATTTTCACTTTTGACCCCTTTCAAGTAATTCTAATATTGCACTTTTATTAGCTATTTCTTTATCAAAAGCAGTTGCTGCTCCACAAGCTATTGAGAATTCTAAAGCTTGTTTATAATCTTTATTTTTTAAATAATTATCAATAAATCCAGCAATCATGCTATCTCCTGAACCAACTGTACTTACAACTTTTCCTTTATATGTAGCACTATAAAAGGTTTGATTATCTTCACTAAATAAATAAGCTCCTTCAGCGCCACAAGACACTAACACATTTCTGGCACCTAATTTTTTAAGTTTTTCAACATGACTTTTTATGTCTTCTTTAGTTTTAACTTTTTCATCAAATAATTCTTCAAGTTCTTCTAAATTAGGTTTAATCAAAAATGGTTTGTATTTTAATGTATTAAGTAGAAATTGTTTAGTTGAATCTACTATTATATAAATTCTTTTGTTTTTGAGTTTTTCTAAAATTAATTCATAAACATTTGTGTCAACATTTTTTGGAACATTGCCAGCCAAAATTAAAGTATCATTATCTTTAATATTAAAAGCAATCTTATCTAATAATTGTTTTAAATTTTGATCACTGATTTTAGGTCCATTAGCATTAATAGCTGTTTCTTTAGCTTTGCCATTTAGTTTTACATTAATTCTTGAATTACCTTCAATATTACAAAAATCTGTTTTAACTTTTTTATCTTTTAAGTAATTAATAATATATTGACCAGTAAAACCACCGACAAAACCTAAAGCTACATTTTTAGTTCCTAAATTATTTAATAGTATTGAAACATTAATTCCTTTACCACCAACTGTATAATTTTCACTTTTGGAGCGATTAATATCTCCTATATTTATTTTTCCCTCTAAAGACATTGTATAGTCAATAGCTGGTGAAAAAGTCACTGTGTAAATCATCTATCCTCCTCAATTTAATTCTATTTAAAAAACTACAAAAAAGCACAGAAAAACACAAAAAGTTACAAAAAACAACAAAAAATAGGCATAAAAGCCTATAAATTTGTAGAATTTTGTAGAATTTTCCACATTTTCTCACATTTTATACTTCTATGATATTCTCAAGATTTAGATCTTTTTTCTTGTAATTAGTTATCAATGTGATGTTTTTATTATTGGTTGCAAAAGTATATTTAAATTCAGAATTTATTTTGGTTTTGTCTAACAAAATATAACTTTTTTTGCATTTTAAAATTGCTTCTTTTTTAAATAAACTTTCATTAATTTCAGGTGTTGTAAAACCTAGATTATTGCTATATCCATTTGCTCCAATAAATCCAATATCAAAATTAAAGTTTTTCAAATTATCTAAAGCTAATGATCCAACAACATTAAACGTATTACTTTTTAATAATCCACCTATTAAAATAGTTGTAACGTTTGAATGACTTTTTAATTCTGTAGCAACATTAATTGAATTCGTAACTATAGTTACATTCTTGTTATTAATTAATTTAGCTAATTGATGAGTTGTTGTTCCTGAGTCAATAAAAATTAATAAGTTATCTTGTACAAATTCATTTGCTTTGGTAGCTAATTTGATTTTTGCATCTAATTCAATATTACTTCTTGATTCATATTCAACATCATTTGCTGAAGAATTATTATTTAATGAATAAGCTCCGCCATGAACTCTTTTTAATAATTTTTGTTTATCTAATTCAGCAACATCTTCGCGTATTGAAGAAATGCTTGCTTGAGTTAATTTTGCAAGCTCTTTTAGTGAAGCAGCACCTTTTGAATTTACTTCTTTTAAAATAACATCTAGTCTTTGTTCTTTAAGCATAATTTTCCTTTATTTATATTTTAATAAATATATTTAAAAAATAAAGTTTTTAACTTTTAAACTAATTATTAAACATTTCTTTTAATTTTAGGGACATAAGGATTAAATTATGTCCCATTTTAAAATCTACAATTTGGAACATAAGAAAAAAACATGTCCCTAAATTGTTTAAGGACATATCTATATTTTTATTCATTTTAGGACATAAAAAAATTTATGTCCTTCTTTATTATTTGTCTTCTTTTTTAATTTCTAAGAATAATTCATCTAATTGTTTTTTATCAACACTGCCTGGAGCTCCTGTAAAAACATCTTGGTTTTTAGCATTCTTAGGAAATGCAATAACATCACGAATTGTTTTTTGACCTGTAATAATCATTGTCAATCTGTCAAGTCCTAAACCAATTCCACAGTGAGGAGGAATACCATAATCAAAAGCTTTTAAGAATCAATCAAATCTTGATTGTTGTTCTTTTTCAATCATACCAATTAAATCAAACATTTTCTTTTGTGTTTTTTTATCAAATATTCTTGCTGAACCTGAACCTAATTCAAAACCATTTAAAACTAAGTCATATGAAAGCGCTCTAACATCCTTCATTTCAATTTTGTCTAATTCTTCTAGTGAATGCGCAAATCTAGTAAAAGGGTGATGGGCTGCTGCTCAAGTATTATTTTCTTCATCATATTCAAACATAGGTCAGTCTGTAATTCAAGAAAGATTTCATTCCTTTCTTGCATAATTAAATCTTTCATTTAATTCAACTCTAACTGCACCTAAAGCTTGAGATGCGTGATCATAAGTATTAGCAACTATAAAGTAAGAACCATTTTTGTTTTTGTTATTTTTGACTAATTCTAAAGCTGCATCTTTAACTTTGTTTGCAAAGTTGGTGTGAACTATTTCACCATTTTCATAAACAAAGTAGAATAGAATATTTGCTTTATTTTTCTTAGCAATTTCTTCTAAGTCTTTAAAGTCTTTTTTGGTAATTAAAGTATCTACAAAAAGCATTCTTTTACTTGGAGCATATTTAATGATATTAAAGTCTGTATTAAGGCAAAAGTCATTGATGTCAACTATTTTATAATCATATCTTAAGTCAGGCTTATCTGTTCCATAATCTCTTAAAGCATCAAAATATTTAACTCTTGGAAATGGAGTCTTGATTTTAATTCCTATTGATTTAAAAATGTGTTTGAATAATTTTTCAATGTATTTTTGAAAGTCTTCAACATTTAAGAATGAAGTTTCAATATCTAATTGAGTAAATTCTGGTTGACGATCTTTTCTACTATCTTCATCTCTGAAACATCTTGCAAATTGATAATATTTTTCAAAACCTGAACACATTAAAAGTTGTTTAAATAATTGGGGGCTTTGAGGTAAAGCTCAAAATTCATCTCTATTTCTAGTAGGTACTAAAAAATCTCTAGCACCTTCAGGTGTACTTTTAGCAAGCATTGGAGTTTCAATATCAATAAAATCATTTTGGTGCATGAATTCTCTAACAGCAAACATAATTTTATCTTTAGTGATAATGTTTTGTTGCATTACTGGACGACGCAAATCTAAGAAACGATATTTCATTCTGGTTTCTTCTTTAACATCAATATCATCTCTAATTGCAAAAGGTAATTCTTCATTTGCTTCAGAAAAAATTGTGTATTTATCAACTACTACTTCAATATCACCAGTAGCTAGATTAGGGTTCTTTTGTTTTCTTAAAACAACCTTCCCTATTACTTCCATTGAACTTTCTTTTGTGACGTTTAAGTCTGTTTTAAAGACACATTGGGTAATTCCATAGCGGTCTCTTAAGTCAATAAAAGTCATTTCTCCAAAACGCCTTTTATTAGCTACTCAACCATGTAAAGTTACTTTTTTACCTTTGTCTTTAGCACTTAATTCATTGTTCTTTATATATTTACTTTTCATTTTTTATTCCTCGTCTTCATCTTCTTCAGCTATTAAATCGCTTAGTAAATTATGTTCTGCTAAAAAGTCTAATAAGTCTGCATAACCATCTTCATTGTATTGGAACATAATACGATCATTATCGCTTAAACTTTTAGCAACAAAGGTTTCACGACCTTGAATGTTGTCATCAAAAATCACAACATCAGCTTGTAATTTTTGAGCCTTATCAAAGATTTTTTTGCTTTTAACTACTTCTTTAATAAATTCAATTTTGCTTCCATAATTTCTTAGCTCATTAGTTAATGAAAATAAAATGTCTAGATTTTTTTCACTGGTTGTGGCTACTAAAATGTTTATTTTTTCAGCATCATTATTGTCTTTGTTTTCATCATTAATAATGTCAGCTGCTCGATCTACTCCAAAACCAAAACCGCAAGCATATAATTGAGGTCCACCTAATTCTTTGATTAAGTTCGAATATCTTCCACCGCCAATTATTGTACTTTGACTTCCTGATTCTTTTGAAGTTGAAACAAATTCATAAACAACTTCATCATAATAATCAAGTCCTCTAACTAGAGAATAATCAACTTGATATTTGATGTTGTTGAAGTCTAAAATTGAAGTTAATTTATTAAAAAATTCAGTGCTGTTTTTGCTTAAGTATTTATTAATTTTAGGAGCTTTTTTAACAAAATCTTTTTGCCTTTCTTCTTTATCATCAAGAATTCTTAAAACATTATTTTCAAGTCTTTCAAGACTAATTGGATTTAATTGATCTTTATATTTAGCAAAATATTTTTTTAATTCTTCTTGATATCTAGTTCTGGTTTCTTCATCACCAATTGAATTAATTTTTAAAACACATTTAACATCAAGACATTGTAATAGTGAAGCTGCTGCAATAATTAATTCAGCATCATTATATGGATTAATAACTGTATCAACTATTTCCATCCCTGCTTGATTAAATTGTCTATAACGTCCTTTTTGTGGTTGTTCGTAACGAAACATTGGACCATAATAAGCAAACTTTGTATTTTTAAGTGTTGCATATCATTTGTTTTCAACTAAAGCTCTAACAAAACCTGCTGTTCCTTCTGGTCTTAGTGCAATATCACGATCACCTTTGTCTTTAAATTCGTACATTTCTTTTTTAACTATATCGCTACTTGCAACTGAACGACGATAAAGAGTGGCTGATTCAATAATTGGAGTTTCAATTAAATGGAAATTATAATTTCTTGCATAATTAACAAATGCACTTCTAATTAATTCAATCACATTATTTTCTTGTGGACCAAAGTCTCTTGTTCCTTTAATTCGGTTAAACATTTTATGCCTCCTAAAATAAATACTCAATTTAATATTAAATATTATATATAAAAGTTAAATAAATGTTATGTTTTTAGATTTAATAAAGTTAATATTTGCTTTTTTACTAGCTTTTAGTATCTTATTTTTACAATTTATGTACTTTTGATAAAATATAAAATAACTATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTACTGCTATGCTAATAGTAATTAGTGGCAGCAATGGTGCTGGTATTTATTTTCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATCTTTTGTTGAATTTTTGGTGCTTTAGCAATTATTGCTATGGCTTTAGCTTTAGTTGAAATTACTAGTGCTAAAAGTCATAATTTATCTTTAATAGGATGAAATCATACTTTTAATAATCGGCATGTATTTAATATGTCAAAAAACTTTATGACTTACTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTACAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCGTTTGTGATTGGCACTAAATATGATTGAATGATTTGAACAGGAATTAGTTTATTGATTGTAATTTATTTTTCAATAGTACCAGCTCTTTATACTAAAGCAGGTGATATTCAAAATAAAATTATGTTAGCTGTTAAATTTTTGCCTTTATTCTTTATTATCATTATTGGTTTTGTTTTGGCTTTTAGTTCAAAAGGTGAAGTAAATCAAACTCATTGGAATTTACAAGATCAACACTTTGATGTTAGATTGGGAAGTTCAATTGCTTCTTATAAATCTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATCTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCCATTTCAATGAGTATTAATGGTGGTTCATTTATTAAAATGAAGGATTATTTAAGTAACTGATGAGGTTCAAAAGCTGGCAGAATTATCTTTGGTATTTTAAACATTTGTATAGCTATAGGAATCTTAGGAATCATTAACAATTTAGCAATGTGATTGCCTCGCTATATTGAAGATTTATTGGCTTTAGGTGAATTACCTTTTTGATATAAACTTAGATTTAAATTAAATAAAGAGAGACCTAAAATTGGTGTAATTTATAGTTTATTTCTTTGTATTCCTTTAATTATTTTGTTTTCAGTTTTTGGAGCATTATGTTATTTTTCAAATGAAAATTACAACATCTTTAATAGCTTAAATAGTGATAAAACTATTAAATCAGATGCATTAAATAAGTTATATAACTTTACTGATTTAGTGGCTAACTGAACATCTTTAATTGTTTATACTTCAATTGCTACAGCAATACTTGGTGGCATTAAAAATAGAAAACATAATTTTGTAAAAGCACAAAAGAAAAAATACTTTTTAGTATCGGCTTACTTTATTGTTACAGTTACTTATTTATCTGTAATAATCACAACAATCATTCCTTTTATTGATTTATTTTTATTGATAGGTTTTGACAGGGAATACTTTAAAACAGTCGAATTGGAAAAATTTGCAAAAGATAATACTTACATAATTAGAAATGAAAATACATTCTTTAATCAACTGCTTAAAGGCAGGATTTTATGTGTAGTAACATTAATAATTTATTTACTTATTTCAATATTTCCAAGTGTTTTTGAAAAGATATATTTCAAGAAAATGTTCAAATCATGAGATCTTTATTTAGCTTGGAAACAAGAAACACTTGAAAAAGGAATAGTTGTTTAAAAATAAAAAATATCACTTAATAGTGATACTAATAATTAGCTCTCATAATGAGAGTTTTATTTTATTTAAAATCTAATAATTGATCTCTGTAATACTTAAATTGTTTACTTCTTAATTCTATTTCGGCAGGTAAACCTAAATTAATTTCTTGAGAGTATTCTGATAATTTGTCAAGAATTGAAACTATCTTGTTTTGGGTTTCTAAAGGAGGAATAGGTATATTTAATTCCAAAATATTTTTCATAAACGGATGCTTTATACTACCACCTCTAAAATTTTTTCTAATTAAATCAAGATTTAAAAGAAAGTAATAATATAAAAATTTTGTTGCAACTTCATTTTTATTTAGAACTTGTCCAATTCTATTATCTCCCGTAACAAATTTACCTTTATGATATTTTATATTTGCCTCTCCACCTCCGGGAATTAACAACACTTCACCTGAATATATATCTTTTTTATAATTTTTACTATTAATTTTTAAATTTTCTTCTTTGCCTGTGGTAATAATTTTTACATCGCCTTTAGGATCATTATAATTTTCTATTTCTTTATAAAATAAATATTTAAAATTAGATGTTTTACTTTGTTTAAATTTTTCAACTTCTTGGAAGTTTTTATCTCAGAATGTTATTTCTCATAATTTTTTTAATTTGTACTGTTTGAAATTAAAAATTTTTGTTAAAGTGAAATCAAATTGCTTCCCTCTCGCTTCCAACTCCGCTTTCAGCTCCGCTTTCAGCTCCGCTTCTAGTATACGAAATCTCTCCAAGATTTCGACAATTTTTTCTTGAATTTTTAGAGGAATTAAAGGAATTTTTAGTTTTTTCAAAATTTCTGAATTAAGGTTTGATATTGTCCCCGGTGAGGATTGTTGAAGAAAAAATGATTGTGAATAATTAGATAAAAATCAATAATATAAAAATTTTGAAGTTACATTTTTTTCAAAATTTGCTATTAATAACCAGCCATCATGAATACAACCTTTTATATCAGCAATCACTGGTTTTCCAAAAGACATTGAATTAGATAAAATTAAATCTCCTTTATCAACAACAACAGATTTTTTGCTACCTTTTTCGTTGATAAATTGTTTAGTCTTTTTTAAATAAATTTTTCCTTCTTCTATATCTCCAATTTTTATTCATGGATAACCTTCTTTAGACAAAAAAGAAGATATAGGTCTAGGAGAGGCACCTCTCCTAATGCTTGATATTTCGCCCAATGTAACCCACTCATATCCATCTGGACAATACTTATCTAATAACTTTTGTAAATAACTCATTTTCTTCCTTTATTAAAAATATATTTTAAACTATTATTTTATTAATGCCTTCCAATAACCATTTTTATTTGCTCCAACTCTTTTAATAAAACCATGTTTTTTTAAAAAATTAATATTGTTGTCTATTGCTGTTGTGCTTAGATTTAATTTTTCTGCTAATAAAGCTTTAGTAATATGTGGATTTTTAATAATTTCCGATAATATCTTTTTTCTTATTTGATTAAGAATTTTTTTAGCTGTTTCTTCATCTATCTCTTCAACTTCAATTTCAAATTTTACAGCATTAATTCTATTAAAAGGAATTGTAACTTTAATAAATCCATCACCAAAATAAATAGCTTCTTTGCCATAAACTTTTACTATTGCAGGTACTCCACGTCCTGATTTTTCACTAATTCTTAATTGAAGAAAAATTTCGGCTAACTTTTCATTAACTGGTTTTGATTCTCCTTTGTAAAAACCTTCTATTGTTTGTTCAGGTCCTAAATTTCCCATAGAATCAATTTCAATTCTATCTTTAAATATAGAAATCATTGGAGAAGCTTTTGAAGTTCATTTGTTATGTAAGATGGCATTAATTATTGCTTCCTTGTAAACATTAGAATCAAATAAAGGTACTTCTTTTCTTGTGAGAACTCTGTTTCTTTCATCAGCTTGATAAATATTTACTACATTTCCAAATCTAATCAAATCATCTAGTGTATAAAGTAAATTAGTAAATCCAAATTCATCTACTGTATATAAAGGGGTTGTTTTGTCATAACCTCCAAAAATAGAAACTCTAACTGGAAATCCAGAATTATCTGACAAAAGCTGAGCAAGTAAGTTATACTCACCTTTTTCGGTATAAAATTCAAGGTTTTGTCTAAATGTTTCTTCTTTTAATTGAATTCCTCTTGACCCATAAAATCCAAATAATTTATTAAATGTTAAATCTTGAGTATTGGCTTTAGTGTTTTGAATTGTTTCATGGCCAAAAATTAAAACTCAAAATAATTTTCTTTCTCTTTCAGGAAATTTCATTAATTTTTCTTTGCTTGATCCAACTCGAATATATCTTTCATTTTTTCAAGCTGTTGGAGTTTTATGAGCTGCAACTATTTTTAAAATAACCAATCTTTTGCCTTGATAATTGAATTCGCTAAATTCAATATTAATTCTTGGACTGAGTTGTCTTTCTAAATAATGAATTAAAGGCTCATTACTCTTTGTATGACTATCATAATTAAATTGTGTACCAACAATTTTATGAGTTTTATCTTCAATACCTCAAACTAAATATCCATAGTCTCTGCCTTCTATAGCGCATGAATTTGAAATAGCAGAAATATAAGAGCCTATTTCATCTTCATCAGCTCTATTAACTTTAAATTCAAATCACTCCTTTTCAAATTCATAAGAAGTTAATTCTTTAATTAATTCATTATTTGTCATGTTTTTTTTCCTTTTATTTTATCGATTTTATTACCAACTTTATTACCCACCTTTAGTATATCAAAATTTTAATTAAAAGTCAATTAAATAGTAAAAAAATGATTAATAAAAATAAACCGTATTTGCCTATAAAACAGGCTTTTTGGTTTATTTGAATTGATTTTCATATAAATTAAAGTAATAACCTTTTGCTTCCATTAATGATGCATGATTTCCTTGTTCAATAATTTGACCATCATCAACCACTAAAATTAAGTCCGCATTTTTAATAGTACTTAAACGGTGAGCTATAACTATTGAAGTACGATCTTTCATTAATTCTTCTTGTAAAGCTTTTTGAATAATTTGTTCAGTATTTGAATCAACATTACTTGTGGCTTCATCTAAGATCAATACTTTCTTTTTACCCAACAGTGCCCGAGTAATTGATAACAATTGACGTTCACCTTGACTTAAACTTTGACCACTATTTTCTATTACAGTGTCATAACCTTTTTCAAGTTTTTGAATAAAGTTATGAGCTGAAGTTAAACTAGCCATTTCATATACTTCTTGGTTTGTGATTTTGTCTGAAGCTACTTTTAAGTTGTCCATAATGGTTTCATTAAACATAAATGAATCTTGTAATACAACTGACATTAAGTCTCGTAAGTTTTTCTTAGTAATTTTTGTTAATTCAACACCATCAATTTTGATTGATCCTTCTTCATATTCATAGAATTTACTAAGTAAATTGATTATAGTTGTTTTACCAGCACCGGTAGGACCAACTATGGCAATAATTTGACCTGGTAATGCTTCAAAAGTTGCATTTTTAAGTTGATATTTACTACTATTTTTATCATATCTGAAGCAAACATTTCTAAATTCAATGTGGCCTTCAATATCTTTTAATTCAATAGAATTTTTGATTTTGGTTGGAAGTTCTAGTTCTAATAAACGGAAAACACGTTTTGTTGAAGCAAGTCCAATTTGAATTGAAAAAATAACATTGAAGAAAACTTGTAAGGTACCTGTATAGTTTCAAAGCAGTGATGTATAAGCTATCATGAAACCTGCATCAGCTTTACTAAAAAAACTAAAAATACTTAAGGTAGGGACATTTTTAATATTAAAGATAATGGCAAAGAGTGAGATTAAAAGAACTAAAAGGTTGGTTGAAATAATAAATCAAGGATCAAAAAATTTAATACAAACATCGCCTTTGAATGAGTGTCAATAAATATCATGAGTTATTTTTTTGAAATTCTTTTCTGCTGTTTCTTGACGATCAAAAGTTTGAGTGATTTTGCTATTAGTTAGCATTTCTTCAACATAAGCATTTAAATCACCAAAGTCTTGTTGTACAAGAACATATGATTTTCTAGCTTTTGAAATCATGAAGTAGCCAAGTGAGAAGAATAAAACTGATAAAGGAATTACTATTGCTGTTAAAGTAAAACCGACAAAAGCCATTGCTAATATTGAAAGAATGACATTGAAAATGTTATTTCAAGTTTCATTGAACATTTGAACAAATGAGTTTGCTAAGTTATTTATATCATTGATTAATACTGAAATTAAGTCACCACTTTTTTCTTTATCATAAAAGCTAACTGGCATTTCAATAAGTTTTTCCATTGCTACTTTTCTCATTTTTGTAGCAGCTTTATAAGCTATGGCAATTAATATCCGACTTTGAATAAACCGTACTATTGCATAACAAATAAAAAATATTGCTAAAATTGAGTTTCAAATAATAAAGCCTTTAACATCGAATGCTCCTGGAATAACTTTACCACTAGCATCAAACATTTTTTGGCTGAAAAAGAGACTAATAATAACACCAGTTAAAGTAGTTCCTCCAACATAGAAAACAGCATTTCAAAAGCAAATAAAGCCACCAATAATGACTAATTTTTTATCTTTTTTGTCTAAGAAATCATAAACTAATTTAAACAGTCTTCAAGTTGAAAATTTTTTTTCTTTTTGTTTATTTTTAGCCATATTGATTATCTCCTTTCTATTGTTCTAGCTGTGTTTCATAGATCTTTTGGTAAAAATCGCAGGTCTTAATTAAGTGACCGTGTTTACCTTGATCCATTACTGCTCCATTAGTTAAAACCATAATATTGTCAGCATTTTTAATAGCTCCAATTTTTTGGCTAATTAATATTGTTGAACAGTCATAATTATTTTTAATATTATTAATAACTTTTCTAGTTGTAATATTGTCTAGTGCACTTGTTGAATCATCTAAAATTAAGATTTTAGGTTTTCTTAATAAACTACGAGCTATTGAAATTCTTTGTTTTTGACCACCGCTAAGGTTTCTAGCTCCTTGAACAACTTTGTGATCTAAACCATCTTCAAATTTATTAACAAAATCATCAGCACAAGCATTTTTAAGTGCTTCACGCATTTCTTCATCAGTTGCATCAGGTTTAGCTCAAAGTAAGTTTGATCTAATTGTTCCAGTGTAAAGTAAAGCTTCTTGGTAAACTATGCCAACAGTTTCATGAAGGTTTTTGGTATTAATTTCTTTAACTTCTTGGCCTCCAATTTTAATTGAACCTTCATCATAAACATAATTATTTAAAAGTAAGTTAATAAATGTACTTTTACCACTACCTGTTGGTCCAATAATACCTAAAGTTTGTTTATAAGGTAATTTAACATTTATATTCATTAGTGAATATGAAGGGCTGGTTTCATAGTAACGAAATGAAAGATTTTGAACTTCTAGATCAAAATTATTTGTTAATTGTTTACCATCTTTAATAAATAAAGGATCTATTTTGGTTTCTAAAATTTCATTAATTCTACCTGCTGAAACACGAGCTCTAAACATTGATGTTAAGAATTGAATGGTCATTAAGAGACCAAAGCTAATTGAAAATAAGTATTCTATAAAAATATTAACTTTAACAAGTGAATCTAAAGTTGCAACTTCAGTATACATTCTATGACCTGCAAAAGCATAGATTCCAATAATTAATAAGTTAATTGAAGCATAAAAAATCGGTACAGCAAAAGCAAAAATCATATTTGTTTTGAATTGCACTTTATATCATCTGTGGTTGTATTCACTAAAACGTTTTTTTCTTGTTTCTTCTAAGTTAAAAGTTTTAATAACTCTTGCTCCTAAAATATTTTCATCAGTTGCTTTAGTAATTTCTTCAACAACTTTTTGTGTTTTCTTTAAAAGTGGATTAGTAGTTAAACCAATAACAAGTACTAGTACAAAAATTACAGGTACAACAGCTAAAACACTAAGTGACATTTTTGGATCGTTTAAAATTGCTAAAACTCCACCACCAATTACTAAAAAGAAACCTCTAATTAATATACTTGAACCATTAACAAGGAATTCTCAAAAGATTGCAACGTCATTTGAAACTCTAGTAATAATACTTTCTGGTTTTAAATCAGCAATATTTTTAAGTGATAATTTTTGAATTTTATTAAACAAAGCATTTCTAAAAAAGCGGCTACTTTGTTCAGCTGGTCACACAATCATAAGTGTTGAAGTAAAAGTTAAAGTGGCTGCTAAAATTGTTTGACAAACAACAGCAATACTTAAATATGTAATTAAATCTTTACATGTTCCTCTAACTTCTCAACGACCATTAAAGAAGGATAATGTCTCAACTTTTGAATAATCATCAACGAATAATAATCTAATAAACTGAGAAATAATATTAGGCAAAAGCAAAGTAAAAATAATATTAATAAAAACAAGCAATGAACCAAAAAGAAATGCTAATTTAATCTTAATTGGTAGCATTTTAATTAACTTAAACATTATTCCTCCTTGCAATATATAATAAATACTTTTTCTATTATAAAACTTTTTAATTATTAATATATATAAAATATATTTAAAATATTAGAATTTATATTAAACTATAATTATTAAATATTTCAAAAGAAATGAGGACTAAATATGAGTGAAAAATCATGTGGATTAGTAATTTTTAGACAATTTACAAAAGGTTGAAAAGTTTTGATTGCTAAGCAAATAAATAATATGTGAAGCTTTGTTAAAGGACATGTTGAAAACAAAGAAAGCGAAATTGAAACTGCACTAAGAGAAGCTAAAGAAGAAGTTAACTTAGAAAATTTTCAAATTTTCAAGGAAACTAGAATGAAAGAAAAATATGTTTTACCTAATGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATGCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGATGAAAAAAAGAATAAATAAAGAATCCTTTATTAAATACTTGAAAGGATTCATTTTTTAACATTAATTATTATTAAAACAAAATAGGTTTGATTAAGTCTCAAATTAATTTAGAAGTGTCTTGAACTAATAAAGTTGCTTTTTGGAATAAATTATTATTACCGTTTGTTATTGCAATGGATTTAATGTTAGCCTTATTTAAAGCTTCAACTCCAACAACTGCATCTTCAATTCCAACACATTCTTCTTTTTTTAATTTAAGTTGTTCTTGAGCTAAAAGAAAAATATCTGGTGCAGGTTTTCCTTTTTTAACTTCAACAGGATTAACAATAACATCAAAATAATTTATAACTCCGAGTTTTTCTAAAATCAAAGGAGCATTGAGACTTGAAGAAGCTATTGCAATTTTAATCTTATCTTTTTTTAAGTCTTTTAAAAATTGTTCGATGCCTTCTAAAAGGCTATCTTTATTAATATATTTTTTTAAATAACTTAAATATAATTCATTCTTTTTAGTACATAATTCATTGATTTTTTGATCATCAAAATTATGCTTAAATTTCTTTAATTCAAATATTGCAAGTAAAGTATCTCTACGAGGCAAGCCTCTTAATTTTTCATTTTCTTCTTTGCTATAATCAATACCTAATTCATTGACTATTTCTTTTCAAGCTTTGTAATGTAATTTAGCTGTATCGGTAATTACTCCGTCTAAATCAAAAATTATTCCTTTAATCATTTTTCACCTCGAAAACTTGCTTTTCATTTGTAATTTCACAGTCCATACCATTAACACATATTTTTAACTTAGAACCTTTATTTATAGTATTAATGGTAAATTTGTCCTTGTTGATTTCTACTAAAAATGGCTCATTTTTGTAAATTGCTTTATAAACTAAACTATTTCAATTATTTGGCAATTTTGGAGCTATATGGAGCTTTTTATTAAATCATCCTAAACCACCAAAACCAAAGACAATCATTTGTCAGATTGCTGCTAAAGATCCTGCATGAATACCTGCATTTGAAGTGTGCATTCATGGACCAAAGTCAATATTAATACCATATTTAAATAATTCATAAGCTTTTTCAATTTTATTTAATCTAATAGCTTCAATACAATAAGTTGCTGCCGAAAGTGATGAATCATGTGTGGTTATTGCTTCATAGTAATCAAAGTTTTTTTCTCTAATTTCTTTATTATATAACTCAGGGAATAAATAAGTTAAGAGAATGACATCGGCTTGTTTTACTAATTGAGAGCCTAATAATCTATGTCCTTCAGAAGTATTGAATAATTTTTTACCAGCATCTCCAAGCATTTGAAATGGTCTAACATTTTTTAAAGGTAAGGATAAGAATGTATCATTTTCTGCAATAATTTTATCTTTATTAGGAGCTTGAATTTTAAGACCTTTAATGACTTTAGTCATTTTTGAAAAATTAATTTTATAAGGTATTCTTTTTAAAATACAGTTAAGTAAGCCTGGTGTTTGTGCTTGCAATTTGCGATATATTGAAATTGCCAATTTCATATTAAATAAGGCCATTTGGTTGATAAATGCATTATTATTAATGTTTCCTTTATATTCATTTGGACCCATTACATCTTTAATTTCATAACTACCATCTGGTTGTATTTCTGCACGGTTTGTGTAAAAGTAAGCTGTATCAATTATCATTTCATAACCTATTTTTTTCATGAATTCTTCATCATTTGTAAAATGATAATATTGATAAACTGCATAGGCTATATCAGCTGAAACATGGATCTCTTGTCTTCTACTTGCAATCGGTACTTGTTCACCTGTTACAACATCAGTTTGGCCTCAATAAGGACAAACTTCACCATCAGTAGGTCAAGCCATTTCTCAAGGATATTGAGCACCTAATAAATGACTTTCTTCATTTCTTCGTTTTGTTTCGAAAGCTTTATTTCTAGCTCCTTTTATCCCTTTATAACGATATGTTAGTAATCTTTTAACAACACTTGGTCTAGTAAAAAGATAATTAGGATTAATAAAGAATTCAGTATCTCAATATGTGTGCCCTTGGTAACCTTCACCTGAAAGTCCTTTGGCTCCTACATTTAAGTTAGTTGAATTTTTAGGCACAAAATTATTTAAGTGAAAAATTGAAAAGTTTAATGCTAAAGCATCATATTTACTTTGTTCATTACCTTCGATTTTGACATAAAATTGATTTCAAACTTTTTGCATTGCAGCTGTAGAATTTTCTTTTAATGAAGCATATGTATTTTTTAATAAGTAGTTAAAAGTTAAATCTGATTTAGCCAAAACTTCACTATTAGTTAATATTTTTTCTTCATCATCAATTGATGTATGAACTGACATTAATTTTTCTAAAACTAATTCTTCGTTTGCTTTTAATTCATTTTCGATTTTAAAGTAAACTTTGCGGCGATCAGTTTGAATTTGATATTTGTCATTACTGCCTAAAATTAACTTATTATTTTTTAAAAGTCTTGTAAACATGTGGTGCACAACAAAACGATTTGAATTAGTTGTTATTTGTTGCATCTTCAAACTTTCGGTTGTCACACGGTTTTTAAGACCTTCTTCAAAGTGTTGAGTACCACTGTTTGTTACTTGTCCATTAATTCCTGGTAATAATTTAACCTTAACAATTTCATCTTTAGAATCTAAAATTTTAATTGTAATTTTTTGAGCATAAATGTTTCTTATTTCTTGAGAAACAAAACGTTCAAAATTTAATTCAATTGTAGCAAAGTCTCTTTTGGCAATGACTTTACGACTTAAAATTCCATTTTTAATATCTAAAGTTTTAGTATATTGATCCTTTTCATTAACTTCAAAAATTTGATTATTAAAAGAAATAGGAGTTTGAATTAAATCAGCTAAATTAGCTAATTCAGGAACTTCTCCTTTAGATGTTCTATTAAAAATACCATTAACAAAAAAGTCTTCTTTGTTATATTCTTGAACTTCTTCATCAACACTTCTAATACCTAAATAACCATTACCTAGTGAAAAAATACTTTCACTTTTTGCTGTTATATTACGGTCAAATTTAATCTGTGAAATCTGATTATTATCAGGGTCATATTCAATATATTCCATAATTACTCCTTATTTAATTTCTAACATAATCGACTCAAAAGGTCTTAATTTATCAGTAACTTTTTTGTTATCAATATATGTTGATAATATTTGTTTTCCTTGAACATTTTCAACTACTTTTTGATGTTTTGTCATATTAATTAAAATAACTATTTTTTGCTTATTAAATGTTCTTGTAATCTGTATTATTCCACTGTTTTGTAGGTCTATTTGACTTTTTCCTAATACTAAAATGTCATGATATTTTTTAGATCTTAGGGCTATAATTTTTTTGTAAAAATTAAGAATACTATTTTTGTCTTCTAAATTATTTTGAACACTAATTCATTGTGCTCTTTGATTTGTTTTAATTCAAGTTTTTTGGCCTTCATTAAAACCATTGTTTACTGCATAATTATCTCATTGCATCAAAGTGCGGCCTGAGTCACGACTGTTGATATTGCAATATTTAATAAATTCGTATTCACTGTAAATTTTCTTTTTATCTACTAACTCACTAAAGGCATTAAATATGTCACAATCTTTAAATTCTTCTCTTGAGTTAAATTGATTATTTAATAAACCAATTTCTTCTCCATAATAAATACAAGGAATACCTTTTAGCCCAAAAAGCATTAAAGCATGACTTTTAGCACTTTCTTTATGAAATATGTTTTCATCTCCTCATCTAGAAATACTTCTTGAAGTATCATGATTTGATAAAAAATTGGTTGTCATACTTGGTAAAACTTTTGTTGAATGTTGAAATGGTTGTTGTTGATAAACAAACTCTTTGTAATCTCAATTTGAGTCATAACCATTTCTACCTGTTTCTTTTCCTCAACCAATTCATCATCAAGAAAAGTTGTAATAGTTATCAGCTACTTTTTTAGATCCTGTTCCATATTTTAATAATTCTTCTAAATTAATTCCACTAGCTTCACCTAATGTATAAGCATCTTTTTTATCACTAAAAGCTAACTTATTAAACTTAAGAAGCATGTTGTCTGCACCTTTACATCATGCAAAACAAGAATTAGTTAAAGTTTCTTCAAAGTTTTTTGAAACGTGTTTAATAGCGTCAAGTCTAAAACCTCTAACACCTAAGCCATATCAAAAATCAATAACATCTGACATAGCTTTAACTGTATCTTGATGATTTCAGTTTAAATCCACTTGTTCTTTAGCAAAAAGGTGAAAATAGTATTTTTTAACACTTGGAACATATTCTCAAGCTGAACCACCAAAAATACTCTTTGCTTCTTTTTCTTCTTTATTTAATTCTTTTCTTCAAATGAAATAATTGTGTTCAATATTATCTTCTGATTCACAAGCTTTTTTAAATCATTCGTGTTCATTTGAAACATGATTTAAAACAATATCCATGATAATATCAATATTTCTTTTTCTAGCTTCAGTAGTCAAAAGTTTGAAATCTTCTAATGTTCCAAATTGTTTTCAAACACTTTTGTAATCTAAAACATCATAACCAGCATCCACAAAATTAGTGTCATAAATTGGACAAAGTCATATCGCATTTATACCTAAGTCTTGTAAATAATCTAGTTTTTTTGCTATACCTAATAAGTCTCCATCGCCATCATTATTTGAGTCATAAAATGAACGAGGAAATATTTGGTAAATAATTTTATCTTCTAATTTTATTGTTTTCATATCTCTCCTATTTTTTAATTAATATATATGAACAATGCCCCAATATTTCTTTCGTATTAAAGATGCCTATTTTTGAGCAAAATAAAACTTTGTATTTTTTTAAATCAATTGTTTTATAGTTAGATTGGTTAAAATTGTGAATGACTCTTACTTCTTCTTTTTCATTAAATTTAATTGAATAATCGACAACATTTTGATCTTTAATGTCTCAATTAAATTTCAAGTATTTGTTGATTTCTTCACTTGAATTTAGTCTGAAATATTTAGTACTTTGTCTAAATTTCAAGAGTCTAATAAATCAACGATAAAGCTCTTTTTTAATTCAAGGATTTTCCAAATTATTTCATTTTAGTCCGTTAGTATAATCTGTCGTTTTATAAGAATTAAAATGGTATTTATTTTCATCTGAATTTAATTCAAAATAATCTTTGGCATATGAATAAAATGACTTATGAAAATCTTGACCACTTTTATCAGCAGGTTTACTTTGAGCTAATTCAGTACCTGCTAAAAAGAATTGACGTCCTTGTGTTGTAATTTGCAAAATCAAAGCTTGGCGATATTGGTCTAATAAATCTTTTATTTTTAAATCTTTATTTAAAGATTCTGTAATAATACGATCTCATAATGTTCCACCATCATGACAAGCAACATAAGCTAAGTTATTTCCAACATGTTTAGTAAATAAATCATTTGAGTTTTTTGAATACTTAAAATCATAACTAGGTCATTTGTAATTATTAATATTGCCTATTACACATGAACGATATTTGGCAACCTTTTCATTATTTACTTTAGTAATCAATCCTGGTCCAGACCCAAATTCATCTCAAGTAATTATTGACCTAAGTGAATCATTAAAGTAACCAAATGAATGATTATTATCACTAATTCCTTTAACATAAGAATCTTTGAAATCTAAATCACTAAATTGTCAAGCTTCACCATGGATTACTAAATTAGGTTTTATTTTTCTTAAGTTTTTAAAACAATAATCAAGTGTTTCTTTATCAATAAAACTTGATAAATCAAAACGGAAACCATCAACATCATAATATTCAACAAAATGTTTTAGTGATTCATAAATTAGTTTTCTAACCATATATCTTTGACTTGCTAAAGGCGGATAAATAACAGGTTTTACTTTAGCATTATCTCGATAATAGTAACCAGGCAGAATATTATCATAAATTGAATTTATCATCATATGGTTATAAACTACATCTAAAATAACTCCGATTTTACTTTTGTGTGCTTTATCTACAAATCTTTTAAATTCTTTAATTCTATTATAAGGATCACTTGGATTAGTTGAATAAGAACCATTGATACTAAAGTAATTTAAAGGATCATATCCCCAATTGTAATTAGTAGCTCAACCTTTGCCTTCTCCTTTTTTAAGCATTTTTATATTGTTTTCATTTAATGAATAAGTTGAATGAATTGGCAAAAGCTGCACATGAGTTATATTTAAATTTTTTAAATATTTAAAAATATCATGTTTTAATGTTGCATCAAAAGTACCTAATTTTTCTTTTTCTTTTGCTTTCTTTAAACTAGTAAAATCACGTATGTGAAGCTCATAAATGATAGGGTCTACTTGACAATTAAATTCGGTTTTAAGAAGGTATTTTATTATGCCACATTTTGATTTATTATCTAGATTAACTATTGCGCATTTTGCAATTTTATCTTCTTTTGCTTCTCAATCAAAAGGAGCTAAAGAATAAGCATAAGGATCTAGTGCAACAGTTTTAGAATTATCTGGATGAGTGATTAAAAATTGATAAAATTTGCCTTCATATTTTTTTTCAATTTTTACAACTCAAACAGTTTCTTCTTTTTCCATAGAAAAACTAATTGTTTCATTAATATCTGAATAATTTTGAAAAATTAATAGTTTTACATCTAAAGCTAAAGGCTGTCAAAATTTTATTTCAATATGATTTTTATGGAATTTTGTTCCTAAATCAGCTTTTTTATAAGCATATTTAGCATCAAAGTCCTTAAAAAATTCTTTAGTTTGTCCTTTTATATTCATAATAATTAAATTTTAAAATTATTTTAAAATAATGTTTCAGAATGCAAAATAATCTTGATGTATCAAGTGTAAAAGTCAATATAAAAAAGCATAAATAAAAAGCACTTTTTTTACAAGTACTTTATTTAATATTTTACAAATATAATTATGATTTTAATGAAAATTCTTGATGGAAAAATCTAAAGTATTTAGGATTAATATAATACTTTCCAGCTACAGAAATGCTATCTTCATCATAGCCAAAAAATGAAACAACTACCAAGCTTTCATATCCAAATTTTTTAACATTTTCATAAGATAATGAATAAACCATATTATTTAAACCATCGCGCTCAATTAATAAGTCGGTTAAAGATTTATTAAAATTAATTTCCTCATCAACTTCAATATTTTTACAGCTTACATAAATTTCAGAAGTTGCTAAAACCTCATTATCTTTATAATAAGTTTTTTCAAAAGTTCTAAAACCTTGAACAAAGAGAATTCTTTTATCTACCATTCATTGAGGTAATTCCTTGCTTTTTATTTCTTTTCCTTCTTGAGCTGTTGCTCCTAAAAGATAACTAATTGGTTTAATTAAGTTGTGAAAGTTTTCAGCAACAAAATGTCCTCTTTTTGAAATTGAATAGATAGCTCCTAATGCTTCTAATTTTTGATATGCTTTAACAACAATACTTCTTGAACAATCAAAACGCATCATTAAAGAATGCTCACTTGGCAAGATTTTGTTTGTTGGTACTTTTTGAGTTTTAATCAAATCAATTAAATAATCAATAATCACTTGTGTTTTCTTCTTTTCTTGTAAATCCATAATATAAATATTATACACACCTTTAAATTTTAAAAAAATAAAGCTATTTTTTTGTTAACTTATAAATACCACAAGCATATTTTTGCATTATTATTCCTCATGGCATATTTTTGCATTTATATTCATATAATGCTTCAAAGTGATAATTCCTGCTAATCTTTTGAAGAACATTATTTTGACATTTATGATTGTAAATAACAAACATTAAATAATTCTTATCATCATTAAGATCTTTACGGACAATAACATTTGAATAATCTTTACTGTCTACTACTAATCTTGGATCAACTAAAGTTTCTATATTAGTTAAACTTAAAAAAGAATAATCTTTTCTTAGCTTATTAATTAAAGCAATCATATTATAGATACTATTTCCTGGTGCTATTTCATTTTCAACATAATCAAGCGAATTAGATACATTTAAAAATAAGTTTGATTGACAAGCGTCTAATTTCTTTTCGTAAATAGCTGATATTTCTTGATGGTTTTTTCACTTCATTGGTTCTCTTAAACCAGGATCATCAAAGCTTCTAGTTCCTGCATATGCTAATTCATCACCATAATAAATTATTGGTTTAGCAGGCAAAGCTAAAAGATAAAGCAAAGCAGCTCTAATACTGTCTTTTTCATCTTCTGTGATTTTAGGATAGTTTCTAATGTTTTTAACTTGGCGTCGATAATTGTCTAATCATCTAAAAGTATCATGATTATCTAAAAAAGGAATTCAGGGTGCTTGAAATTTTGTATTTTGGTATTTAGCAACAAGGCTTTGTAAATAAGAAAAATCTAAACGCACATCATCATTATCTCTAAAATGTTCTCATCCATCATAAGTAGAGTCTAGTGCTTTTTGATTATTATAGGTAAAATATTCAAAAGCTTTTAATGCTTCACTTAATCACTCACCCATCATAAACACTTCTTCACGATTTTGCTTTTGAGTGATTTCCTTGCTTGCTTGTCTTAAAAGATGAAATATTTTTGCTTCATTAAAATTGGTTTTAGTTTCGCTTTCAGAAGAAAAGTATTCACTAAAGGCATCATATCTAAAACCATCAACACCTAATTTAGTTCAAAATTTTTGAATAGCACAAAGTTCATTAATTACATCTGAATTATTTAAGTTTAAATCAGGCATACCGCCTCAAAAGCGACCAATAAATTTTTTATTTGTAGCTTTAAAATTTTGATCAATGTTTTTATATCTTTTTCTTAATTTTTCATTATCAACTCTTGCATCTAAATCATTTTTAAGTGGTTTGAAGTTATAATAATTTTCATATTTTTTGTCACCTTTTAAAGCTGCTTGAAATCAAGGATGTTCATAAGAAGTGTGATTAAAAACTAAATCTAAATAGATTCTTATACTTTTTGAATGAGCTAATTTTAGGAATTTTTTAAATGCTTCCATGCCGCCTAATTGAGGTGCTACATCACAGTAATCAATAACTGAATATCCATGATAACTTGATGAAGGATGAATTGGACTAAGTCATATTTGGTCAATACCTAAATTTACCAAATAATCTATTTTATTGATTAATCCGTAAAAATCACCAATGCCATCATTATTACCATCCGCAAAATTGTAAACCAAAATTTGATAAATAACATTGCTGCTTAATTTTGATTTATTAATACTGCAAGTTTCAATTTTTTGATCTAAATTTAAGTAAGTTAAATTTGGATTTTTTATTAAATATTTTGCATCATCTCAAGATACATAATTTTGGTTTGGATGTTTTTTAATGAATTTTTTAAGTTTCATTTTTTTAATAAAGTTCATAAATTTTTCTTTTAATTTGTTTGTTTTCATAATAATAATTTTAACTTTTTTAAATATTAAAATATTTTTTAAATAAAAATCTTGATGTATCAAAAAAGATGCCGAAGCACCTTTTTAATCATTATATTAATTGTTTTTCTAAGTCCTTAGTATCAATTTTGCTTTTAGCAATAATTTGGTCATTTTTAACATATCCATATTTTGTTTTAGCAACAATTAACAAAATAAAGTTTGTTAGTATAACGAACATTCCAATTCATGCATAAGTAATGTTCATTTTATCTAAAAATTGATATATAGCATATTCTGAACCGCTTAATTCTTTAAAGTCAATAACTTTGTTAAGTTTATTAGGACTTAGTGAGATTGCAAAAAAGAATAAACAAAAAGAGGTTAATTGGAGAAATATAAGATAAATAAAGTATTTGTCTGCATTACTTTTATTTGAGAAAAGTCGAATTACTGCAATACAATTAATGATTAAAGATATGAAGAGAATATTTATATTAATCATTATTAATGCAGGAAGGAAATTTTTAAGACCATAAGAAGGAGAGTTTGCAAAAGAACTATTGTAAATCTTTTCTTTAGTTGACTTGATAGCAGCAGGATCTACATCTCGAAGTCAATTTAATTCTTCATCTATTCTTTTTAAGAATAAAAAGAGAAAAGCAAACAATATTGCTATTGCAACTATTGTAATAATAATTGATAATACTAATAAAGATTTACGAGCTAAATAATTTGTTTTAGATTTTTTTAATTTTTGGTTAACTTTGAACATTAATTAATCCGTTTTGTTGTTTCCGCATCGAAGATATGGATTCTAGGTTCATAAAATTCAATGTAAACTTTTGAATAAAGCTCGTAAGTATCTGAATTGCTTGCTGTAATTGTAAGTTCGATATCGTCATTATCCAATTTAACAGTTACAAGTTGTTCTTTACCAATTAATTCAATATTTTTAATAGTTGCATGAATTGAATTTGATACTTCAGAAGCATGAATTTTGATGTCTTCACTTCTAAAACCAATTTTTAATTTTTTGTCTTTTAATTGTTCCAAATATTCTTTTGGGGCTTTAATTTTAATGTCTTTTTGAGCACTAACAATTTTGCCGTTTTCGCATGCTGCTTTGAAAACATTCATGGTTGGAGAACCAATAAATCTAGCTACAAAGACATTAGCAGGTTTGAAGTATAAGTCACGGCCAGTACCATTTTGTTGAATTGAGCCATCATTGAAGACAACAATTTGATCACCCATTGTCATAGCTTCAAGTTGGTCATGAGTTACATAGATACTGGTTGCGCCAAGCATTCTGTGGATATTAACAATTTCACGACGCATACTTTCACGAAGTTTTGCATCAAGGTTAGAAAGAGGCTCATCCATCAAGAACACTTGAGGTTTACGAGCAATAGCACGACCTATTGCCACACGTTGTCTTTGACCACCAGATAAGTCACGTGGTTTTCTGTATAAGTAATCTTCTATTTTTAGAATTTTAGCAACATCCTTAACACGTTTGTCAATAACGTCTTTTCTTTCTTTGGCTATTTTTAAACCAAAAGCAATATTATTGTAAACGTTCATGTGAGGATATAAAGCATATGATTGAAAAACCATTGCAATATTTCTTTCACTAGGTAAAAGGTTATTATATCTTTTGCCATTGAATAATAAGTCACCTTTTGTAATTGAATTTAATCCCGCAATCATTCTAAGTAGTGTAGTTTTACCGCAACCACTAGGTCCTAAAAAGATACAAAATTTACCTGGTTGAATATCTAAATTAATATTTTCAAGTGTATAATTTTCATTACCTTCATATTTTTTAGATAATCCTTTTAGTTCAATATGAGCTCCTTTTTCAATATTTCTAAGCTCACCGATTTCATCTAGCATTTTGTCCAAATTAATTGTTTCAAAGGATAAATCGTCAAAATCTTCATTTTGATTTTGAGTATTATTCAAGTCTTTATTTTTCTTTTTAAATCACATATTTTATCCTTTCACAGCTCCTTCAGATAAGCCACCAACAATGTATTTTTGTAAGTACATAAATAGACAAAAGGCAGGAAGAGAAGCTAGTATTGAGCCAGCTGCATAAGCACCAGCATTTTTATGTTTTTCTTCAGCATTAAGGAAAATATCTAATCCAACAGCTAAAGTTTTTTCTTCGTTACTTATTAAAACAAATTTCGGTAAGATAACATCCGTAAATGGAGTTAAGAATGATCATAGAGCAGTCATAATTAAAGCAGGCATAATAACTGGTAGAAGTATTTTGAAGAATAATCCTCAGTTAGAACATCCATCAACTCTAGCTGAGTCATCTAATTCTCTTGAAACTGTATCTAAATAACTTTTTAGCATAAAGGTATTTGAAGATATAGCTCCACCAGAATAAATAATAACCAGCATTAATATTGAATTAATTCCTAAATCATTCCCCATCCGCACAATAATGTAAAGACAAATAAGAGATGAAGTGGCAGGAATCATTTGCAACATCATAATAATAGTTAATGAGTGTTTTGAACCAGCGAATTTAAATCTTGAATAAGCATAAGCATTCAAAGCAACACAAATGGTTGAGATAACCATAGTTAAGAATGCTAGCAATAATGTATTACCATATCATCTTAAAAAATAGCTTCGTTCACTTGTAAATAAATAAGTAAAGTTGTCAAAGCCAAATTTAAAAGGTGTTATGGCTACAACCCTTTGGTTGTTAACATTTAGTGAGGCCACAATTAGTGAAACCGCAGGGAAAAGCACAATCAAGGATCAACAAATTAAGATTAAGTAATTAAAGAACAGTCAAACTATTTCCATTGGTGTTGGAGGTTTAGTATCTGATAAGTTATAGTTGATACGAACTTTTTTGTTGATAACTTTTTTAGTTGAAATGTTATCAACAATTGAATCATAAATTTTGTTTTCACTATTCATTATTAGTCCCTCCTTGTCATAGATTTAATGAATCCTCTAGCTGCTACAGCGATACTTAATAGAGCTGCAAGAGTTGTCAAAGCTGCTGCAAAAGCTTGATCAGATTCAATTTTTACTGCACCAGTGGCTAGTTTATAGATAAATGAAATTAGAATATCAGTTGCACCTTCTTGATAAGCAGTTGGTTTAGCAAACGCAGGGCCTCCGCCACTAAAGATACTAATAGTTGTGAAGTTGTTGAATGCTCCAACAAATTGACCAATTAACATTGGGGCAATTGCTAATAATAAAGAAGGTAGTGTTAGCTTAAAGAATTTATGATAATTTTTGGCTCCATCAATAGAAGCAGCTTCATAAATATCTTTTGGAATTGCTTGTAAGTTACCAGTAACTAACATGAAGATAAAGGCATAAGCTATTCAAGTTTGAACAACAATTAAGATAGCTCGAGCTGAGGTTATGTTGTTAAGTCATTCAATAGGTCTATTAACAAAATGAAGTTTTAATAGAATCATATTAATATAACCTGTTGTTCCATTAACAAAAGCACTTCTTAAGAAGTACAACATAACGAAAGCTGGAATTGCTCAAGGAATAATAAAGATTAATCTAAAGAATTTTCTTCCTTTAAGACGATTATTATTTGCTAATATCGCTAAAATAATACCGAGTGAAATAGGACAAAGAGTTGAAGCGATTGTTCAAATAAATGTTCAACCAATAATTCTTTCTAGAGCTGTTAGAATTCCATTATTACGATAAATTCATCAAAGTCCTCATTGTTTCAAGCCAACTCATGAACTGGTTTGAGTAGGGGCTTGATGCATGTATCCATAGTCAGTAAATGAAATTAGAACTGAAGTAATAATAGGAGCTAGAACAATAAATAACATTAAGATTCAACCTGTAATTGAAATCATTCAAGGGAAGCCGCTTGTATTTAATCATCTTTTTGTATATGATCATTTACTTGGACGTGACCCTTCTTCCATTAATTTAGCTGTTCTAAATGCAATAATTGAACAAACTGTAAAGTAAATTAATACTATTGTTAAGATAATAACAGATATGACACCACCAAAAAGGTAACGCCGAGCATCTCTAAATATGCCCATGCTAGAATTGAAGTGTCGAGCTCCTAAGTCAATGAAACTCAGAATCCCTTCCATACCATCAATACCAGTAGTATAAGCTCCGAAACTAAAAGGAATAAAAATTGCATACAATAATGTGGTTACTAAAAGTAAAAGAATCCCTTTAGTTTTTTGTTTGAAGAATATCAATTCTGAAAGTCCAGGGAAAATAAAACTAATTATTGCTGCTAATCATTTAATATTACGTGTACATTCAATTGGTACAGTTTTAACAGCTTCATTAACTTTACTTTCATAAATTTTTGAAACAACCTTCATTTCGGCTTGTCTTCTAAAGAATCAAGTTGATAAAATTTCTTTATTTTTAAGACTGTTAGTTTGAAGTTTTAATTCATATCTTTTTTCATTATAATCAATCTTTGCTTCCATTTTACTATGTTTAAAAGCTTGTTTTGATATTTCTTTATTTTTATATCTTAAAGCTGTTTGCTTAACTGTTTCAAGATATTGATTATAAAGTTGTTTTTTCTTGTTAGAAACTAATTCTTTTTCGCTTTGATATTGTTCTTTGTTTCTAACTTGTTCTTTTAAAATGTTAGAATTTTCATTTGAGCTAAGATTTAAAGAATTTAGTTTTGTTTCATAAATAGCTAAAACTTTTTCATATAAGAATTTTAGTCTTACTTGCTCAGCATTTATTGCTCTTTCTTTTAAGGCTAAAGCTAATTCTTTTTTATAATTTCCTTCATAAGAATATTTTTCATAAATTGCCGATTCTTTGAAATAAAGTTCAGATTTAAGCAATCATTCAAATTCTTTTTTTAGGCTTTTTGTGTTAAAAAAACCAAAGAATAATTTTTTAAATATTTTAGCTAAAGCAGATGTTTTTTTGTCATTATAGACAAGTTGTTTATTTTTCTTAAATGACTGTAAAACAGATTTTTTGGTAATTTTTATAGATTCATCTTTATTAGAATCTATAAAGTTATTTTTAGCTAAAAATTCTAAATATTGTTGGTAATTTCTAGTAGCAACTAAATCATCGATTTTCTTTAGTTGTTGCTTGAAATTTTGATTTACAATCTTAATTTTTGATTTTCCATTTTGCTTTATTTTTTTAATTTTTTCAATATTGCCTTTTTCAACACGTTTAATCTCATTTTTGTGTTCTTCGATTTTAGCATATGCTTCTTTTTTATATGCTTTTACTTCTTGTTTTTGTAATTCTGCTTTTTGATTATATTCATATTCTAAAGCAAGATTTTTTTGATTTGTTTCTAATTTAATGTTGTATTTGTTTGCTTTATAAATCTTTAATTCAATAAATTTATTATTTTGATATTTGTGGTTTAGTGAATTACGTTTTTCAATTAATTCTAAACGACGTTTTTCCAATTTTTGATAAAAGTTTTGGAAATAAGATTTTGGGTCTTCTAAAGTATTTAATATTTTTAGTTCTTGTTTATGCAAATAGTTTTTAATTTTATTGATGTCAAAATCAAGATCTGAATTATGTTTAAGATATTTTAGAGTTATATTGAATAAAGCATATTTAGAAAGAATTTTATTTTCTTCTTTAATTGTTTTTTGCTTTAATTCTTCTAATAATTCATTCTTCTTTTCATCAATGTCAGCTGTTAGTCTTAGTGATTTTTGAAATTCTAGTACATATTTTTTAAGAGCCTTTTTTTCTTTTATTAACGCTTTAATTTCATACTTAATCATTGAGATTTCGTTATCAGCAAACGATATCTTTTTAATAGCATCTTTAATAGCTTTAATTTTATTTGAATAAGCCGCATATAAACTTGAAAGTTCTCTTTTTAAGTTATCTTGTAGTTTTGTTCTAGCTCGTAAAAATAAATTTTTGTTAATCTTCATTTGTGAATTAATTTTATCGATACGCCTATTAACAACATTTCTAACATGATGTTTATAGGCTTTTAAGGTATCTTGTTCTTCAGAAAGAATTGTGTCAAATTCTTCTCCATATCAGTTAAAAAGTTTTAATTTTTCCATATTAAATGTCCTTTATTCAATGTGTACCATTCATAATGGTGCATTCTTCGAGCTAAATGTAATTTGACTGTTAAAAAGATTAGATAAGAAAGTATTATTACACAAAAGATTGTAATAAATACTCCTATAATAATTTCTTGTTTTAATAAAACTCTAGTGCCTCTAAATCAAGGCAAAGCTCCTAAAAGAGCAAATACTAAAATTCATATAATTAAAAACCCATAGCCTGCTTTTAATTTATGCCTTAATAGAACAATGTAAATTAGAAAGCAAATTAATGCTAATGAGTTTGCAATATAGGAAACAATTTTAGTTATTGTCACTTGTTTAGCAATTTGTGCTTTTTCTTCATCGCTAAGACTTGTCTCTATAAATTTAGAACCATAAACAAAAGGAATTAAGACTCAAAAAATCATTAAGACTGTAATTGCTGCTAAACAAGAAACTAAAAAGTAAAAACTAAATTTGTCTATATTTAATTTGGGTTTAATGTATGGATTTTGATCCATTATTCAAATCTAATAACAGCTGTCGATTCAGTTGAACTATTAATTGTGTCAATTTTTTCTTTGTAAATTGCTGTTAATTTTTCATACATCACTTTTTTAAATGCGTCAAGTGTTTTAGTTGTTGTTGCAATTTCAGAATATAATAATTTGTTTCCAAATACAGCATCATTTCATGAACTTCATGCAACATTACCAAATTGAGGATTTGATTGGTCAATACGTCCTGGTTGGTATTGAATTACATTCAATCCACCATCAGCTAATGATTTAGTTGAAGATAATGTTTGATAAATTAAGTCAACTGTTTGATTTTTTCTATATTGTTCAACAATTTCAATAACTTTAGAAGCTGGCACTTTAGTGCTTTTATCTTTTGCTGGAAATTGAAATTCTTTAGTTAATTTGTCACCAAGATTTCTAACTTCAGCTTGGTTAATGTATCTTGCGTAAAGATATGCTTTAACTGCTTTTGTAATAACTTCATCAGCTACTGCTTTTTTAGATAAAAGTTGTGAAACTTTTTTAGCTTCAGCAATTTTTGCATTGATTAAATCTTGCAATTTGTTTTCTTTTAATGCTGTTTTGAATTTTTCGACAACAGCATCGTATTCTTTACCATTGTCACCATCAGCACCAAAAATGATTTTTTCAAGCTTTCTAATATGGAACGAATTGTCACCTTGTTTTGCATTTGCAAATAATTCATTTAAAACATTTGCTTCGTTTAATTTTGTAAAAGCTACTTGTCAAGTTTGGCCATTACCTTGTAAATCCGAAAGTTTTTCCAAACCTAAAATTGCTGCTAATGCATTACGTAATCCAGTACCTTTTTCAGTATATTTATCACGAATATCTTTGAAATTCATGCCTAATTTAGATTCTAAAGTACTGTTTAACACAGCATGTACTGGTAAATTGTTAGCTGAATACATATTTGAATCCGCAAGTGGGTCATCATTCGCTGTATTTTCTCAAGTACTTGCACTTGGAGAATCGCCTACATTTGATAAATTATTAATTACTTGAATTGATGAATCATAATCTCTATTAAATTCAGCAGCATCTGTATATTTCAATTTGCTCATTAATTCTTGTAATTTTGCATTTTGAGCGGCTGAATCTTGATCAACTTTTGTTTTTAATTCTTTTTGTAAGTCTACAATAAATGGAACTTTTGAATCGCTCTTGAAGTATTCATAGAATGATTGAGAATCAAATACACATTCAAGAAATTCTGCAATAGCTTCTTTTCTTGCTTTTGATTTACCATTATTTCTAATGTTTACTAAATATGATCAAGAACTTGAAGCTTGACGGTATGTATAAGCGTCATTTAATTTGCCTGCATTAAAGAATGTTTTGGCATTACCTGATTTTTGAGCTACTTGAACATCTCAAGTACCAATTAAACCATATTCTAGTTTGCCTTCTTTCATTAATTCAAATACTTTGTTATTTACAGCGCCCATGTCATTACTTAATAATGCTTTTAAGTCATCTTGTTTAATATTTCTTTTGCCTCATACTGAAGTAGCATATGCTCCTGAGTCTAGGATATATGCAGCTTCATATATAGGAAAGAAGAATTCTGACATTAAATCAACTGCTTTTTTGAATTCAACATGGATAGGATTTTCTGTGTAAAGACCTGTTGAAATTTTTCCATTTTTTAAGAAAAGTAATTTCTTCATCATATCTTCAGCTTTGTATTCATGAGCTACAGCGCCTAAAATACCATTACCATATCAGAAGTCTTGGAATCTTAAAAATGCACGACCTTGTTTTACTAATTTTAGCAATGTATCATTTTCAGGTTTAGCTAAGTCTTTTTTAACATCTGCTAAAGATTTTGTTGAAGCAACTATAACACCTTCAGTGTTGTGTCTAATACCAAAAAGTTTTTCAGCTGGTAATTGAGTAATTTTTTCAATACCGACTACTGAACCAAATGATCTAAGTTGTTTCTTTTCTTCTTCAGTTGCTTTAACTTTTTTAGCAATACGATCTAATAATCCTGGTGAAAATTGATCTAAATCAGCAACTGCATTAGATGCAACTAAAGTTGTAATTCTATCATGTGGTGCATAAAAAATATCTGGCACAATTTTTTCATCTGTTGCCCCTGTAGGCATTTCTAAAGCACCTCAAACATCTTTAGCTATTGTTTTAATTTCATAGCCATTTTGGCCTGATTTTGTTTTCTTAAACAATTCAATTGCTTTGTCATAGAATTTTTGTTGAACGCCATCAACTGCAATTAAAATTGTTTTGCTTTCAGTAGTACCACAACTAATTGTAGCTAATGCTGTCACTGGAGCTGCTAAGGTTGCAAAGCCAATTGCTATTTTTTTAAAAGTTTTCTTAAAATTACTCATAATTTCTCCTTGTTAGCTAACTATTCAAAAACGATTAGATTTTTTTCATAAGCAACTATTGAAAAGATAATTGCTAGTGGACTAAAAAAAGTTAAGACAAATATTGTTGCAATAATTCCGCTTCATTTGTAAAATTCGGTTTTATCCAAAATATAGTAAACATGAATGCAAACGAATATTGCAAATATATAAGAAAGTAATGCAGCTGAAAATAAAATTCTATAAGTATAACTTTTTATTTCGCTAACTATGTTTTCTAAATATTTGCCTTCAAAAGTTCATTTATTTTTGCCTAGTAAATACAAAATTAATATTTCTAAAATCAAAATAATGATTTGAGTAAAAACTAATAACATGTATTCAAGAATTTTAGGTAATGATCTTCTTTCATTTGGTCATTTGCCTAAAAAAAGTCATCTCATTCTAGTTTTAAATGAAACTTTTGTACTTTTTTTGTTTTCTAATCTCCTGTTTTTTTGCATAATTAACTAAATAAATTATATTTTAAAATTAAAATATATGCCTTGTCAGTGTGCAATTCTTGATTAAATATAGGAACATTACCCTTGACCACTCAAGATAATAAAAAATTCAAAAAACAACAAATTTTAGTGTAAAAAATTTCTTTTTGCTGTTTTTTGTGCATTTTATATATTCTTATTCTTAATCTAACTTAATCTAATTGTGGAAATTTTCTCATATATGAGATTTTAATTTTTAATAAGTTAATTCAACTAACATTAAACCAGTATTCCCACTAAAAGTTATTTTTTCATTGTTTATCGAAGAATTATTAGAAAAAATAACATTAATTTTAGTTGGAGCATTCTTAAAGACTAGATTTCATTCTTGACCATAAGCTGTATGATAATAAATTGAATTGACTAAAACTAAAAACTTTTTATCTTTTTGCTTTGCTTTAACTAATTTATATCCAAGTCCTTTATAATCATAACCGGGTTTAAAGAGATCATCTAAATTAGCATAAGTATTTTCTTTTTCAGCAAACATTTTTGGATTTTCTTTACGATAATTAATTATTTTTTTGGCTAAATTAAAATTAGATTTTTTATTCTTATGAGCTTGTTCAATCGAATTCTCATTCTTGGATAAATTCAAAAAGATATGACTAGTTTCAATACCCGAACGTCTTTCATAAAAATCTACTTGGTTTTTTTATTACTTCATTTAAAAGCTTCTCTTAAAAAGGCATCAGAATGTGCTTGTGGGCCACCTCTCATATTTAATTCATTACCATTATAAAGAATTGGATTGCCACTTTGAATTAATAGGTTACTTAATCCATAACTCATTACATTTAAAACTTTAAAATCATTTTGCTTAATTTCGAGATAAAAAGTTTTAACTCCAAAAATCAATTGGTTATTATAGTAGCGCTTGTTAATTCATCTATCTTCATCATGGTTATCTAAGAATGGTAATCATGTTCTAATATGACCTTCAACTTCATGTTCTTTTAAAATTTTTTCATAATCTTTTTTTGAAGTTTCAATAAAAAAACCATTTTTAAAATGAGAACCATCAATTACAATATTTAAGCCAATTCTTTCTTCATAAGGTTTTGTTGTATCATTAATTGCAAAATATTTATGAGCTTTACTTGGTTTATTTCATCATTCACCAAAGAAAAATATATCATCGCTTGATCTTTGATCTTTAGTTTCTTTTATTACTTCTCTTAAGGCTAATCTTTGCTCATTAAATAATTTTCTCTTTTCTAAGCCTGTTGCGTCTTTAAATTCATGTTTATTTTCTGAATTCATAATGTAATAAAAAGCATCAAATCTAAAACCGTCAACTCCTTTTTTAACTCAGAATTTCATAGCATTAATAATTTCTTTTCTTGTTTTGGAATTCTTTAAATTTAAATCAGGCATACCGCCTCAAAATTCAGCAACATATCTTTTATATGAAGACTTTTGATTAGAATCTATATTGTAAAATAAATTTCTTGTACTAGAATCATCAACACCATATTTCTTTTCACAATTATATTGGAAGTTGTAAAAATTATAAAATTTGTCAAATTCGTCTTTGTTTTGAAGACCTGCTTGGAATCATGGATGCTCAAATGAAGTATGATTAATGACTCAATCCATAATAACTTTTATGCCTTTTTTATGGGCATTTATTAAAAATTGATCAAAGGCTTCCATGCCACCTAATTCAGGAGCAACATCTGTATAATCAATTACATCATAGCCATGATATGAGCTGGCTGGATGTATTGGTGAAAGATATAAAGTATCAATTCCTAAATTGCTAAAATATTCTAAATTTTCATTTAGTCCAATAAAGTCGCCAATACCATCATTATTGCCATCTGCAAAAGAATAAATTGTTAATTGATACATAACATTTGAAGGCTTAATAGATTTGTTAAAAGGCACAATAAATTTTGCATTTTTAAATTCACCTTCATAAGACAAATTCTTTATATTTTTTCAACAAATTTTTCATTGTTTCACAATTAATAATCTTTTTTAACACTTGTGCAACTTGCACTTAAACATAAAGCGATAGGTGCAATTAAAGAAAAATATGCTAATTTTTTTCTTGTTTTTTTACTCATTTATACCTTCTTTAATACAAATATTATATTAAAATAAATTATCTTAATAGTACAAGCTTGCAAATCAAATAAAGAAAAATTACCAAAATGCTTATAAAACAATGTTTTTAGTAATTTTAAGTTATTTTAAAAACTTAATTCAACTAACATTAATCCTGTATCACTATTGAAAATAATTAAATATTTTCTATTTTAGAATTATTAGCTGCAATTACATTAATATTAGTTGGTGTGTTTTTAAATTTAGTTTCTAATTGTCAAAATTGTTTGTATGATTTTTTATTGTGTTAGCTAAAACAAGTATTTTATTGACTGCTTTTTTATCTTTAAGATAGTATTCTGTATCATTGCCATAGTTTCTAAAATTATCTTTAAATATGCCATCTAATTTGAATTTTGGATAAACATATTTTGTTAATTCAAAAGCAAGACCATAGGTTTTAGAGATCTCACTTTTGAATTAACAAAATAAATATAAAATTTCCAGCTTGCGCTGGATTTTGCCAGCTTACGCTGGAGTATTTTCTTTTGCATTTTTTCACTTTCATATGAAAAAAATGATTTTTAAATTAAAATTTAAGCAGACAATTTAAGAAAATGCATTTTTCTTAAAACTTGCCGCCCTCACTTTTGGAAATAATAAAGTAATAAATTTTGATTCTACTTTTATTATATTAATATATAAATTAATATAATTTATTGCTTGATATATCAATGTCAAAAGCAAATAAATAAAAAAACTAAAAAGCCTGTTTTATAGACTTTTTAGTAAATTTTGAGTAAAAATAATTAATATGAAATTTTGCTATTTTTTTCCACAAATTTTTCTTTTAATAAAATGGCCACAGAAAAAATTGGATTCATTATTATTGAGTAAATTGGGATAGTAATAAGGCTTTTTAAAAGGATTGGAATAGTGATTAAGTAATAATGCTCACCAGTATAATTTAATGACTTTCTGCCAGCATAAAGATAATTGTAATAATTAATGTAAACATAAGGCCCAATTAGTCATCTAAAAATGATCATAATAAAAATAATAATACTGAAGACAAATAGATAATCAAATCATCTTTTTTTCACTTTTTTATTCTTAGCAAAAAGCAATTGAAAAATAATAAAAATAAATGCGATAACACTGAAATTAAAATATATTAAGAGCATTCATAAAACTGCATTTTTAGGTAAATATTTTAGCTTCTTAATTCCTTTAATATGATAACCTTTATCATTTCCACTATTTATAACAACATAGAGTAATAAAGCGAAACCAACTGAAATTACAATTATTGGAAAAATAATTCTAAATAAAAAACTAGCACGATAAAAAAAGTAATAAAAGGAGCTTAAAATACAAATGCCTATTGGAATCAATGCATAAAGATAATACCAAGTTCCAATTGTACCAGTTAAAAGCATCATAAAAGTATCAGCTAAAAATGATATTAAAGATCCTTTAAGTGGTCCAAAAAAGATTCCATATAATATAAAAAAAATTGCATCAGTTTCTAAGGGTATTATTTTTAAAATTGTAAACTTAGAAATTATTGCAACAACAAAATGAATTGCCAATAATATACCTGCTAAAGCAATATCTAAAACACTAAGACGAAATGACTTAGCGAAGTGTTGCTTAAACTTTTTGCCAAATGATAATTCATTAAAAGATTTGTCATCTTCATTGGCTGCAATATTATTGGTAAAATTCTCATTTTTTTGCATATTTTGAATTATATGTTAAAAATTTTTATTTGTTATTATATTATATATATTATGAACAAAAAATTAGAATTTGGAACAGCTGGAATTAGAGGAATTTTAGGTTCTGGTGAAGACCATTTAAATGTAGCTCATGTTCAAAGAGTTATTCATGGTTTTGCTAAATATTTAAAAAACAAATATAGCCACATTAATCCAATTACTGTTGTTATTGGACGTGATAACCGTAGATTCTCAGCTACATTTGCAACCTTAAGTGCTCAAATTTTAAGCCAATATGACATTAAGGTTATTTTTCCTCTATACTTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGAATTGGAATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGACGATTCTTGACTTGCACTAAGACCATCAGGAACAGAACCAAAAATCAAATTTTACATTTTTGCATTTGGTAAAGATCAACAAGAAGCTCAAGCAAAATTTGATAATTTTGCAAGAATTATAAAAGAAATAAAATAACCATAATATTAAAGCAAAAGGATTAGCCTTTTGTTTTATTTAACTGTTTTATAGGCTTATACAAAAAATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAACCACTTATGCCTTCAACAACAGTGTAGCCATGTCAATAGTTAATTTTCTTAAAGTACGCTAACACTAAATCTGGTGAAGGCCCACAAGAAATTTCAACTGCAACTTTTTTATATTTAGGATAAATAAAGTTAATTAAGAAGTTAACAACCAATATATAGAAGAATGTTGTAAATTCTCTCATTCCGAAGAATACTCTGTTACCTGAATCTTCAATAATTTGAGTTCCAACACCATTTGTTAAAATTTTTGCATAGTTATGATGTGGTTGAGCAAAACCAAAAATGATTAAGAATGTTATAGTCATTATGATTGAAATAAGACTTAAGATATTGCCAACACTTTTTTGTTTTTTAGTTGAGAAGTAATAAGCAACAATATCTGTGCCGCCAGTTGATCCGCCATTTTTTCAAGCAATAGCAATTGCTGTTCCAGCACAACCTGAACCAATAGCTCCATAAGCAAAAATTGGTCAAGAATTAGGATTTTCTAAAGTTACACTTCCAACTTTTATTTCTTTAGTTCAACCAGGTGCAAAATTAATATGTTCATGTAATCAGTCTAAAACTATTTTATTTTCTCCAATTGGAACTGAAGTTAATATCAAATTAGTTAATATTTGAAATAGCATAAAAGCTAGTGTCATCAAAGTAAAACTTTTCTTAATCTTAAATCCAAATCCTAAAAGAAGCGGTAAGTTAAAAGCTAAGTAAATTAAAGCAAAATAAGGCTTAGTACTCGGTGCAATTAAGTTAATTAATAAGGGAGCTCCACTAAGTCCACTAGGAATAGTTTCACCTCTATTTAAGAATACTTGCACACCAAAGTTAAAGACCAATGCTGATAAAAAGATAAAAACTAAACGACGAGCATAGAGTTTAGCAATTCTTTTCCTAACTTCCTTGTTTTTAGGATTATCTTTTTCATAATTATGACGGACCATTTCAAGACTGTCAAGAGATTTTTCATCAAGAATTTCCTTTTTAGGTTCTTGGATTTGATTTTTTGCTTCAAAAACCATATCTTTAACTTCTTGAATAGCTTCTTTACTAACTTCAAGGTTATGCACTTGTTCTAGTTTGGTAGTATCTTTTTCGTTGTTATTCATAATATTAATTTTATATTAAATATTTATTTTTTTAATTAATATTTTTGTTATATAATAACCATGTTACTTTTGTAACCATTCTGAACAGGTTTAAGAGCTTATAAGCCACCTTAAAGATGAGCCACAAAATTCAGGAGGTATAGCATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGCCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAACCCTAAATCAACTCATAAGAGAAAATTGGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAGGATTATAACATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCGGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAATTTAGATAAATATGCACATTTTTATGATGTGCATATTTTATTTATTTGAGTGTTTTATAGGTTTTTAAGAAAAAACAGGACTTGCCTGTTTTAACTTTCTTTAGCAAATTTTCTCTTTTGAAAAATCATTGCAATAATTGAAATAACAATTGAACAACCTGCGACAATAATTGTTAAAATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGAGCTATTGATGTTACAAAGTAAACAATGACTGTAGCTGTTGAAAGAATAAAGAATAAAGCAATTGCAACTAAAGCAATAATTGAGCCACCAATTAATGAAATTGAAGTTGAATTATGAAAAATGTTATTTATAAATTCTTTACAAGCAGCAAATCCATCCTTCATTGAAATAGCAACTGCGCACATATTTCAAGTGCCAAAGAATCCTACTCCAAATTCAGTTGTTGCTGGTTTGGCTTCTTCTTTGATGATCATTTCAGGTTGCATACCAAAAATGAACAATAAACAAAAGCCAACTACAAAAGCTAATAATACTATTAGCGGAATAAATTTAGTTCTACTATACATTATTATTTTCCTTTTTTTAATTACTATAATTATTTTATAGTATATATTTTAATTTTTTAAAAAATAAACAAGACTTTAAAACAAGACCACTATGGGTCTTGTCTAAATTTAGGCAGATTTTATTTCTTTCCTCTGTAACCAAAGTCAATAACAGCACGTCCTAAGAATTCACCTTTTTGAAGTTTTTCAAATACTTCTGAAACTTCTTCTAGTTTAATAACTTTTGTAACTTCTGATTTAACTTTTCCTTCTGCAGCATATTGAAGTGATTCTTGTAAATCAAGTCTTGTACCAACGATTGATCCAGCTAATTCTTTTTCGAACAATACTGTTCAGAATACTGAAACGCCAATGTTGTCTTTACCAAGTTTGTCTTTTGATGGAAGGCCTACTAATACTTGACGTCCTCCACGACGAAGCATTTCCATGCCTTGTTCACCAGCAATTGTAGCAACTGATGTGTTAATTACAGCGTGAGCTCCACCACCAGTCTTTTCAATAAGTTTTTGAATAAAGTTTGGATCTTTCTTAGAGTTGAATGCAAATTCTGCTCCTGATTTTAAAGCTAATTCGCATTTTTCATCTGTTAAGTCGATACCAATAGGTCTATATCCCATTGCTTTAGCATATTGAATTGCTAATTGGCCAAGTCCACCAACACCGATAACAACCATTCAGTAACCTGGACGAGCTTTAGCTCTTTTAATTGCCTTATAGGTTGTAACACCAGCACATACTACAGGTGCTCCAGTAATTAAATCTAATCCTTCTGGAACAATTCCTACATAATCTTCGTGTCCAATTGCATATTCAGCAAATGATCCATCAAATGTGTAAGCTGACATGTTTTGTTTAGGACAAAGTGTTTCTTCACCTTGTAAACATCATTCACATAATCCACAAGCATCATGTAATCATGCTAGACAAACTCTGTCACCTTTTTTAAGACGTGTACATCCTGGACCAAGTTTTTCAACAATACCGATACCTTCGTGTCCTGGAATTAATGGATATTTAGGTTCTACCAATCAGTCATAGTTTGCAGCGTGTAAGTCTGTGTGGCATACCCCTGAAGTTTCCATTCTAATAAGAACTTGTTTAGGCCCTGGTTTTGGAATTGGAACTTCCTTAACACTTCATTGTTTTGGTTTTTCAACAACAAAAGCTTTCATAGTTGTTTTTGCCATAAAATCTATCTCCTTAAAAAAATAATAGTAATTTTTTAATGTTTTATAAAACATTCATGGGAACATTCCCATAATTTAATTCTATATTTTTTTTAAAATAAAATTGCCATTTGAAAAAAATACTAAAAGTTTTCTACAATAGAGTGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTTAAATTTAAAAAAGTAAAAAGAAACAAATGAAATAGAGATATAAAAGGTTATTTAAAATTAAAACTTGATCAAAAGATAAAAATTATCGAATTATATTTTCAAGAATTTAGTATTTTAGAAATATCTAAAATAATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTTTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAACAAACAACTCAAAGCATGTCAAGAAAGGGAAATTGTTTAGACAATAGTCCTACTGAATGTTTATTTAGTGTTATAAAAAGAGAATTTTGATTTGGAGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGGAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGTCCTGTCCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATTTTTGGGGTTCATACCACAAAAAACAAAGATTTTTATTTTTTTTAATAAATTATGTAAAAAATGGCTAGGTATATATAGGAGGAGAAATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAAGAAAAAAATTAATCAAGCAGAAATAGATATAGTATTAAAGGAGCTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACGAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAATTTTTGTCGTCTAAAATTTCATTAATTGCTTGACCAATAGCGCCAGTTAAATTAGAACCTATAACTCTATCGGCAGCTACTTTTGCTTCTTCAGTGGCATTTCCCATTGCAAAACCAATGTGAGCATTTTTTAACATTTCAATATCATTGCCACTATCACCAAAAGTATAGAGCTCATGAACATTAATTTTTAAATAGTCTAGAAGTGAACTTAAACCATTTCACTTAGTTACACCTAAAGGATTAACATCAGTGTAAATTGAGTTTGTTAAGAAAACTTTTTGTTTATCTTTTAATTTATTTTGAAAATACTTTGTGGTTTCTGCTGCAGTAATTAATGGATTTCTTAATGCCATTTGAGTAACTTTACTATTATTATCTTTAAGAACTTTTTCTAAATCTGAATATGGTTTTAAGAAATTTGAATTTTTATCTGTTAATTTTGTTTGATCTTTAACTCATTCTGGTAATATATTTTTATCACTATTTCTAGATCATGCACCATTAAATTCAGGAGTATCAATAGTTAAAATTAAATCATGAGCTTGAGCATAATCAAAAAGATCATTAAATATATCTTTATTAACTTCGCCTAAAACTTTATAATAATCTTTTTCAACATCATAAAATAATGCACCATTTGAACATACAAAATAATTAATGCCTTTAATTGATTTTAAAAGTGGTAAAGTTTTTTGCAAACCACGACCAGTACAAATGATATTTGCATGTCCTAAATTATATGATTTTAAAATTGCTTCTTGAGTTTCTGGATGAGTATTAACTCCATCTGTTAATAATGTGCCATCCAAGTCAAAAGCAAACACTTTTCTTTTGTTTTGCATAATGCGCTCCTTACTATATTCATTAATTATATTATTTTACCATTTTTTTCAATCTTTTTTAAAAAAGACTATTAAGGCAAATTATAAACCAGGACAAAAGAATTAGACTTAAATCCCAAAAAGATTTAAGTCTTTTTGTACTAGTTTATTATTCTAAAACATCTGTTTTATAGGCAAAAAATAAAAAAGCATGAATGCTTTAATATTTGAAATATGTGAACTTGTATCAGAATGGTAAGAAATCATATTTTGCACTTTGACTAAATGCCACAAATAGAATCAAACCAACTATCAATATAAAGAATAAAATATCTAATCAACCTATTCTTAATTTACGATATCTTGTTCTTTTTGCATAAGGATCATAACCCCTAGTTTCCATAGCATTTGATAAGTCTTCCGCTTTAGCAAAAGAAGTAACAAACAATGGAATAATTAATGTTGTAAATGATTTAACTTTTTCTTTAATTTTTCCATTCTTAAAGTCAACTCCACGACTTGCTTGAGCTTTCATTATTCTTTGTGCTTCTTCAAAAAGAGTAGGAATAAATCTTAATGCAATCGAAATAATCATAGCAATTATGTGAGTTGGAATGAATAGAAGTTTAAATGGAAACATCAAGTCTTCAAGTGCTTTAGTAAGTAGTATTGGTTTGGTTGTAATCAATAATAAAGAAGTAACCATAATCATAATGTAAATTCTTATAATTAATGCTCCAGTTCTTGCTAAAGCTTCTAAAGTTAAGGCATATTCACTATGGCCACCTCAGGGTATTATTCTTGTATAGTCCGAGTCAGGTTTTAACTTCATAGTATAGATATTAACAAAGAGAATAAAGAAACCAATAATTAGAGGCATAATCATTAATCTAAATAAAGGATATATTTTTCTTGAACCAATAACAAATGCAATCATTAGAGGTACTAATAAAACAGCTAAAGAGACAAAGTAATTAACAATAAAAGTTAAAACAATATAAATAATATTGATTATCAATTTTAACCTTGGATCAATTTTATGAATTATTGTTTCATGTGGATAATAACGACCAATAGGAGATTTCATTATACACCTCCTTTCATTCTTTTATTTAAGAATGCAGCTAGTTCATCAATAGATGTAACTTTAGGCACACTAATGCCTCTTTCCTCTAATTTAGAAACGAATTCTAATAATTTTGGAGGTTCCATATTGTTTTCTTTTAAGAATTTTGTATTCCTTAATACTTCATAAGTATCACCATCTTTAACAACTTTTCCATCTTTAAAAATTAATACTCTTTTTGCTACTTCTAAAACATTATCCAAATCATGTGTAATAATAATAACCGTTTTACCTTGTTGATGAAGTTTATTAAAAATTTCTAAAACTTCTCTAACTCCAACTGGATCTAATCCAGCTGTTGGTTCATCAGCAATTAAAATGTCGGGTTCCATAGCTAAAATTCCAGCTAAAGCAACACGCCTTTTTTGACCACCACTTAAACCAAAAGGTGATTTATTCAAGTAACTTTCATCCAAACCTACTAACTCAAGATATTTTTTAGCTTTTTCATAAGCTTCAGTTTTATGCACACCAAAGCTTCTTGGTCCAAAAGCAATATCATCTTTAATAGTAGATTCAAACAATTGGTATTCAGCAAATTGAAATACTATTCCAACTATTTTACGAATATCTTTAGATTTCTTAACTCTTTTTTGTCTTGCTTTAACAATATATGAATCAATAAATTTTTCTTTTTCTTTTGTTTTCTTATTTATTTTTTCATTTTCAAAAATTCATTCAATTGAACCATTTCATGGCAAAATAAGAGCATTTAAATGCTCTATGAATGTAGTTTTACCTGAGCCAGTTTGTCCAATTATTCCTATATATTCGCCTTGATTGATTGTTACATTAATACCACTTAATGCTTTAAATTCCATAGGAGTTCTACGACTAAAAATATGTTCTAAATCTTTAACTTTTATTTGCATAGTTGATTTAATAACTCCTCTTCATTATATGTTGATTCAATATTTTCAATTTTTTCACTGAGCTTATAAATAAAAGGTGAATCAATTTTAGCTAATTCAATGATTTCATGATTTTTTAAAATTTCTTTAGGTGAGCCACTTGCAACAACTTGACCTTTAGAAAAAACTAAGCAATAATCAGCTTGAATAGCTTCATCCATATCATGAGTAATTGAAATTAAAGTTTTTTGACGAGTTTTTTGGATTTCTCTAATAATTTTTAAAACTTGACTTTTACCTCTTGGATCAAGCATTGAAGTTACTTCATCAAAAATTATGATTTCAGGATTTAAAGCTAAAACAGAAGCTATGGCAACGCGTTGCTTTTGACCTCCTGAAAGGAATTGAGGTTCTCTTGTCAAATAGTCCTTCATTCCTACTTTTTCAGCTAAATCATCAATAATAGGTTTCATTTCTTTTTGTGGAATGCATTGATTTTCTAAACCAAAGGCAATATCATCTTCAACACTAGCGCAAACAAATTGGTTATCAGGGTTTTGGAAAATAATGCCAATTTTCTTTCTTATTTCTCTGATTGTTTTTTTAGAAATAACTGTTCCATTTATTTCAATAGAACCTGAAGAAGGTTTATACAAAGCAACAAGCAATTTACTGAATGTACTTTTACCTGATCCATTGTGACCTAATATAGCAACATATTTACCATCTGGAATGGTAAAATTAAGCCCATTGAGAGCTTTTGTTTTTGATTCTGGATAAGAAAAAACTAGGTCCTGTACTTTAATCATAAAAATATTATATTATGAAATGATTTTTTTAAAAACCAATAAAATATTTTTTATTTATAAAAAACAAAAATGTTTAATTTTTATTATTTCTAAAATACTAAATTCTTGAAGATATAATTCAACAATTTTTATCTTTTGATCAAGATTTAATTTTAAATAGCCTTTTATTTCTCTATTTCATTTGTTTCTTTTTTAAATTTGAATTGCATAAAAGTGAACCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAATGTGCCTTATTGTGTGATTAAAATAATCAAATTATGTATGTTGAATTTAGTCTAAAACAGCTATAAAACTGTCTTATTGGTAAAAATTATTATTTAGTTACTTTAGCATCTTTTTTGCTTTTTGTTGCTTTTGATGCTGTAGTTTTTGTGTCTTTGTCTTTTGATGTTTTTTCTGCTTTGTTTGTTTTAACTTTGTCGCCTTTAACTAATTCAATAATAGCCATAGGTGCATTGTCTCCATCACGTGAAACCAATTTGTAAATACGTGTGTATCCACCATTACGGTTTAAGTAACTTGGAGCAATTTCTTCAAATAAAACTCTTAAAACATCTTTACCATCTGTGTTTTTAATTGTACGTAAGAAACTTGCAGCAATACGACGGTTTGCTAAAGTTGGGTTTTTTGCTTTGGTAATTAATTTTTCTGCATGGCGACGTAATTCTTTAGCACGAATAAGTGTTGTAGTTAAACGTCCGTGGATGAAAAGTTCACTAACTAAGGTACGAATAACACCTTTTCTTCATTTAGTATCACGACTATAAATTTGTTTTGGGTTAGCCATTATTCTTCTCCTTTACTTAGTTCATATCCATATTCTTTTAAACGTTGTTGAATTTCATCAAGTGATTTACGACCAAGGTTTTTAACTTGTTCTAATTCTTCAAGTGACATTTCAGCTACTTGAGAAATCTTTGTTTTACCAATTCTTTTAAGTGCATTCAATGAACGTACCGAAAGATTTAATGAATTGATATCAACATCATTGTCGTTTGTTTCTTGAGTTTGTTCACTTTCTTCTGCAAATACATTTACTTTCATTTCATCAACATTACCAATCAATTTGAAGTGACCAACTAAGATTTCACAAGCTTGTTGTAATGCACTCTTAGGATCAACTGTTCCATCTGTTTGGAATGAGAAGGTTAATCTTTCTTCAATTTTGTTTGATGATGAGTTTAATTCTTCAACTGTGTAGTTAACTTTTTCAATTGGACTAAAGTTACTATCTGTAGCAATAAAAATTCCGTTTTTGATTTTTGATTCCATTCTAGTTGTAATTGAAGAGTTATTAAGTAATTTCTTATTTTCTTCATTAGAAATAAAGCCACGACCTGGTCTTAAATATAATTCAATATTTAATTTGTGTTTACCATTTGCTGATAAGTGAGCAATTTCTAATGATTTGTTTAAAATTTCAATACTTGAGTTGTTTACTTCTAATAATCTTGAAGTAACTTCTCCTGGTTGATCAGCTTTTAATGTTACTTTAATAATTTCGTCATCTTTAACAAATTCTGGGTTGTATTGGAATTTTACTTTTCTTAAATTCATAATAATTGTGGCAACGTCTTCTACGCAACCATCAACTACTTGGAATTCATGATTAACATCTTCAATTTTGATTGCAAATAATGCTAATGAAGTAATGTTTGAAAGTAAAATACGACGAAGAGCAACACCTAATGTTTGACCCATACCACGTTCAAGTGGTTGAAGAGTAAATGCAGTTACATTTGAAGTTGAATTATTTAATTTAGTAACCTTTTGGTACTCTAATTTTGTCATTTTTTCCATAATTATTCAACTCCTTTACTATTTTTTAGCTTCACGTTTAAGAATACGTTTTGGAGGTCTTGTTCCGTTGTGTGGAACTGGTGTAACATCTCTAATTTCTGTTACTGTGATACCGGCAACTTCAATTTGTTTTCTAGCAGCGTCTTTACCTGCACCTAAACCTTTTAATTGAACTTTTACGCTTTTAATACCATGTTCTTTGGCAGCTTCAGCAGCTGCTTGTGCAGCTAAACCAGCTGCATAAGGGGTTTTCTTTTTGGTCCCTTTGTAACCAATAGCACCTGAAGAACTTCATGCAATTACGTTTCCTTTTTCGTCAGCAAAGGTAACAATAGTATTTTGGTTTGTTGAGTGAATGTGCGCTACACCATTTGTTATATTTTTCTTTTTGGTTTTTCTAGCCATAATTAATTCCTCTTCCTATCTATTATTTAGTTGATTTTTTACCTGCAACTGTTTTACGAGGACCTTTACGTGTACGAGCATTCTTTTGTGTACATTGACCTCTAACAGGTAAACCTTTTCTGTGACGAATACCACGGTAGCATTTGATTTCCATTAAACGTTTGATGTTTAAACTGGTTTCACGACGAAGATCACCTTCAGTTGTGTATTCTTTAGCTACTTCACGAATTGCTGATAATTGTTCTTCTGATAAATCTTTAACTCTAAGATTTTCATCAATTTTTGCTTTAGCACAAATTTCTTGTGCTCTAGTTCTTCCTATACCATAAATGTATGTTAATGAGATAACAACACGTTTATTGTTAGGAATTTCAATATTTAAAATTCTAGCCATTTAATTCCTCTTATCCTTGTCTTTGTTTGTGTTTTGGTTGTGAGCAAATTACCCGAATAATTCCTTTACGTTTGATAACACGACAATCTTTACACATTCTTTTTACACTAGCTCTAACTTTCATATAATTCTCCTATAAATCTATTTGTGACGATATGTTATTCGTCCTAAAGATAAATTGTAAGGACTAATTTCAACATCGACAGTATCTCCAGGCAAAATACGAATGTGATTTACACGCATTTTTCCTGAAATGTGAGCTTTAATGAGCATGCCGTTTTCAAGTTCAACATCATATTCATCTGTTGAATAAGCTTCTTTGACAACAGCTTTCATTTTGATTGCGTCTTTTGCCATTTAGATTCCTTTCGTTAATATAATACCTTTGCCATCTTTAATTAAAACAGTGTGTTCGTAGTGAGCTGCATTTAAACCGCTAGCGCTAACAACGGTTCATTTATCTTTTTTAACTTTGATTCTAGCATTTTGTTGTAAAATCATTGGCTCAATACAAATTACCATTCCATCTTTTAATAATGGTCCACTACCAGCATGGCCATCATTAAATACATAAGGGTCTTCATGAACTTCACGTCCAATTCCATGCCCACAGTATTGACTTGGTGTATATAAATTATTTTGTCTTATTACTTGACCAATAGCGTGACTAACATCTCCCACTCTTGCACCAGGTTTAATCGCATTAAAACCTGCATAGAATGATTCTTGAGCCACTCTAATTATTTTTTTATCACTTTCATTGATATTACCAACACCTTTAGTGAAGGCACTATCGCTGTTCATTCCTTTGTAAGTACATCCCATGTCGACTTTAACTATGTCGCCATCTTGAATAATGTAATCACTTGGAATTCCATGGATCAATTCTTCATTTATTGAGATGCAACAGGTTGCTGGAAAACCATATAAGCCTTTAAAAGCTGGTTGTGCACCTCTTTTTACTATTTCATTAAAAGCGACTGAATCCACTTCTTTTAATGAAACCCCTGGTCTTACAAAGTCATAAACTATTTGTTTAACTTCTGCCAGGATTTTGCAGCTTTGAGTAATATTAATAATTTCTTGTTCATTTTTAATAGTTATCATAAGCAGCTTCTTTCTACTTTTAGCAAATTAGTTTTTTAATTTCGCTAAAGATTCAACAATATTTTTTGCTATAGTTTCTGGATCACTTGTAGCATCAAATGTTAATAATAAATTATATTTTTTGTAATAATCAAGCAATGGTGCTGTTTGATCATGATAAACTTTTTGACGTACTTTAATAGCTTCAATTGAGTCGTCTTTTCTTAAGATTAATTCAGCTCCACATTTATCACAATATTTGCCTTTAGTTGAAGGCATAAAATCAATATGGAAACTTGCTTTACATTTAGGACATTGTCTACGTCCATTTAATCTTTTTAAAATAACATCTTCAGGAGCACTTAATTCAAGAGCTACATATTTAAAGTTTGAAATACTATCTAAAAAGTTAACTTGGTCAATAGTACGTGGATAACCATCAAGCATAATAATCTTTTTAGCTTTGATTAAAGCTTGAATTTTATTTTTAACAATAGTATTTGTAATGTCGTCTGGTACATATTGTCCTGCTTCAACTATCTTTTTAACTTGTAAACCAAGTTCACTTTGTGAAGCTATTTCTTCACGAAAGATATTACCAGTTGAAACATGTACTAGTCCATATTTTTCAGCTATTATAGCAGCCACTGTACCTTTTCCTACACCAGGAGGCCCCATAAAAACTATATGTTGTTTAATTTTACTTATCATAATAAGCCATCTCCATTTTTGTATTTATCATATTCTTTTGAAACGTCTTCAGATTTTTGATCATTTGTTGCAAAAGGATCATTTTCTTGATATTTGTTTTGGAAGTTAAGCACATCTGCAGCTTCTTTTGAAAGACGTTTTTGTTTTGCAAGATGTTGACTTTTACGGCGTGCCATGAATTGTTGAATAGTTTCAAGAGCAACTGATACTAAAATCATCATACCTGTACCACCAAAACTTACTATAGCTGGAATTTGTAATCATAAAATCATTATGTATTGGAATGAAGCTAAGAATACTAAATAGAAACCACTGAAGGTGCAAAGTCTTAAAACAACACCTATTAAATAATCTTCAGTTTCTTCTCCAGGTCTAATACCAGGAATAAATGTTGAGTTCTTTGCAAAGTCTTCTGAAATCTTGTCAATTCTTGATTGTTGCAATCCAATTATGTAACTAAAGAAGAATGTAATTAGAATCAATAATGACAAGCCTAGTGGTTGAGTGAATTGCATATTGGCTTCAATTCATAATTTGCCAGAATTACCTTTTGGAAGTATTCTAGATATCATAATTGGGAAGCTGACTAGCATTGAGGCGAAGATTATTGGCATAATTCCACCGGGATTTAATTTAATTGGCAATTTACCCATTTCATTAATATTTCTACTACGTCCAGCACCAATTTGTTGAATAGGTACATGTCTTTCAGCATTGTAAACTAAAGCAATAATAAATAACAATGCAAAGTAAACAAATAAGTAAGTTAAGAATTTTAATGCTCCAACAAAAATTGCTGCTGTACTTTCACTACCAACAAATAAGCTAAATGCTCCTTTAAATTGACTTGGTAATTGGAATGCAATACCAACAAAAATAATTAAGCTTGTACCATTTCCTAAACCTTTATCTGTAATTTGTTCAGCAATGAATAATGAGAACAATGATCCACCAATTAAGATTAAAGGCAATATTAAGTAGATTGTAACTTCTCGAGGGAGTTTTTGAATTGGAGTAAATCCAATAAATGGATTGTCACCTAAACTAAGTGATTTAGTAAGCAAGATAGCTTGAGGGAATGCAATAAACAAAGTCAAAATTCTTGTAATGATATTTAATTTTCTCCGACCTTGTGGTCCACTTTGACTAAGTTTTTGAATTGGAGGGAAAAGTTTGGTTTGTAAAAGAGACATTATCAAGCTGGCATTAATGAATGGACTAATTCCAAGTGCTGCAAGTGAGAAGTTTTTAAGACCTCCACCGCCAACAAGGTTTAATGTATTTAAGAATGGGTCATCATTAATATTTACATCAGATAATTTAACAAAAGGAGCTTTGATTGTTGTCATTAAAATATAGATTGATAACAACAATAATGTATAAAGCAATTTTTTAGTAATTTCTTTAGTACTTCAGAAAACTATTCATTTGTTTTTGAAGTTATAAAAAAATTTTGAAATCTTAAATCCTACATTTGAGAAAAATTTCTTCATATATTCAACCCTCACTTTCATTGCAAAAACCAATTCAACTTTGTCTCTTATAAAATACTTTATTATTATATAATAAACTACCAAATTTTATAGTAAAAGTTTAATAAAAAAACTTATATTTTTTTAGCCTTAAAATAAATAAAAAATATTTTAGTTTTTTAGTATTTTAAAAAAATAAAAAAATTTCACCAAATCCTTTGTTTTATAGGCTTTTTGGTAAAAAAATGAATAAAAAAATGTCACACTCGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAGCTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAAGTCTCACAGTCAAGCACACTTCTACCTTTGCGCTCTACATACGGTTTCTGACCGTACTGAGTGTACCTTTGAACGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCGTCACGCACTGTCCACCCACCTGATGATGATGGCATGTTAGAAACTCAATATACCAAGGGTGGTATTTCAAGGATGACTCCACGAGAACTAGCGTCCTCGCTTCAAAGTCTCCCACCTATCCTACACATGTTAGACCAAGTTCCAATACGAAACTGTAGTAAAGCTCCATGGGGTCTTTTCGTCTTGATGCGGGTACCCAGCGTTTTCACTGGGACCATAATTTCACCGAGTCTAGTGTTGAGACAGTTGAGAGATCATTGCGCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCATTTCAACGCTTCGCAAATGCTAACGCATCCACTTAACCTTCGGGCACTGGGCAGGCTTCACCCCCTATACATCACCTTGCGGTTTAGCAGAGAGCTGTGTTTTTGATAAACAGTTGCCCCTCATAATTTACTGTGGCCTAAGTGTTACCTTAGGCGCCCCTTCTTGCGAACTTACGGGGTCATTTTGCAGAGTTCCTTAACACTAGTTTTCTCGCTCGCCTTAGAATACTCATCTCGGGGACGTGTGTCCGTTCTCGGTACAGGTTTCCATAATATTAAGTTTAGAAGCTTTTCTTGGAAGTGTGGAATCATCTAATTCGGTTTGACCCTATGCATCACGCCTCCCGGTTATAGACTGCGGATTTGCCTACAGTCACCAGTGAACGCTTACCCCACAATCCAGTAAGTGGTAAGATTATCCTCCTCCGTCACTCCATCACTATTATAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCGTCTTAGGTCCTGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCGTAGATTCTCACTACGAATCGTTACTCATACCGGCATTCTCACTTCCTAGCGCTCCACCAGTCCTCACGGTCTGACTTCATTGCCCTAGGAACGCTCCTCTAACGTAAAATAATTACCCGCGGCTTCGGTATCGTGTTTTAGTCCCGTTAAATTGTTGGCGCAAGGTCTCTTGACTAGTGAGCTATTACGCACTCTTTAAAAGGTGGCTGCTTCTGAGCCAACTTCCTAGTTGTTTATGAAACCTCACAACCTTTCTCACTTAACACGATTTTGGGACCTTAGCCGGCGATCTGGGTTGTTGCCCTCGCGAGCCGGGACGTTAGCACCCCGGTTCCGACTGCATAGCAATACATAGCGGTATTCGGAGTTTGATTATAGTCAGTACCCCTAGGCGAGGCCATTCCATATTCAGTGCTCTACCACCACTACTTAACACTACACGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCACTATTCACAAGTCATCCGGGCACTTTTCAGCGTACTACGGTTCGGCCCTCCGCTTGGGGTTAGCCAAGTTTCAGCCTGCTCATGAATAGATCACATGGTTTCGGGTATATGACAACATACTAAGACGCCCTATTAAGACTCGATTTCTCTACGGCTCCGCTTTTTTCTGCTTAACCTCGCATGCTGTCATAACTCGCCGGTCCATACTGCAAGATGTACGCCATCACCCATAAACGGGCTCTGACTAATTGTAAGTAAGTGGTTTCAGAATCTATTTCACTCCCCTCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGGGTTCCACGTGCCCCGCCCTACTCAGGATACGATCAGAAGACTTAACAATTTCACATACGGGGGTATCACCCTCTATGCCGCTTCTTCCCAAAAGCTTCTGCTATCATTAAGTTTTGTAACTTCATGTAGATCGTCCTACAACCCCCATTGCTGGGTTTGGGCTCTTTCTCGTTCGCTCGCCGCTACTAAAGAAATCATTGTTTATTTTCTCTTCCTCTTGCTACTAAGATGTTTCAGTTCACAAGGTGTCTCACTCAAGTTCCTATGAATTCAGAACAAGGCAACTAGGCATTACCCTAGTTAGGTTTCCCCATTCGGAAATCCCCGTTTCATAGCATATTTCCGGCTCCACGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTTCAGACCCAAGGCATCCACCACAAACTCTTCCTTATTTAAAAGTATTTTCCTATTGTTATAGTTTGTTATGATGTGTATTTTAAGACATTTCAATGAATCATAATTAATATGATTTTCTCGATGAAATCAAAACAAATTAACCATTTAATTTAATTTTGATGTCGTTGTAAATATCAAAAAATTTACTATTCAGTTTTCAAAGAACAAATGAGAGAAAAAATATTGTTCTCTCAAAACTAGATATAAAGCTTTAGACCCATAAAAAAGCCACATAACAAAAAAATATATAAATAAAATAGGTCTAGTGAAATAAGGTTAATAGTACTTTAAAAAGTACTCTTTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACCCCAGTCACCAGTCCTGCCTTAGGCAGTTTGTTTATAAACCGACTTCGGGCATTACCAGCTCCCATGGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGTAGCGTAGCTGATCTACGATTACTAGCGATTCCGACTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGACCGGTTTTTTGAGGTTTGCTCCATGTCACCACTTCGCTTCTCTTTGTACCGGCCATTGTAGCACGTGTGTTGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCGATTACTCGGGCAGTCTCCTTAGAGTCCTCAACTGAATGGTAGTAACTAAGGATAAGGGTTGCGCTCGTTGCAGGACTTAACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCATTCTGTTAACCTCCACTATGTCTCCATAGCTTTGCAGAAGATGTCAAGAGTGGGTAAGGTTCTACGCGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGATCCCCGTCAATTCCTTTAAGTTTTATTCTTGCGAACGTACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGCCGATGAGTTCCCCATCAGCTAATGATCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGTATATGTCCAGTTAGCTGCCTTCGCCATCTTGGTGTTCTTCCTTATATCTACGCATTTCACCGCTTCACAAGGAATTCCGCTAACCTCTACACAACTCTAGCCTGCCAGTATCCAACGCGTTTTGGGGTTGAGCCCCAAAATTTAACGCCAGACTTAACAAACAACCTACAGACGCTTTACGCCCAATAATTCCGGATAACGCTTGCAACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGTTGCTTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTTTGTCGCATTATTTTGTAAATGCTTTAATATGATAACACATAAATTAATATTTGCAAAACATTTTTTTGAAAATTTTTTTATTTTTTTACAAAGTATTTTAGAAATTATTTTTTTATGCTAATTTAACTTTTTTAAATTATGAAAAAATAAAAAAAGTGCCTTATTTTTAAAGCACATCTCTTATATATAAATAAAGAAAGGAATTTTTTAGAATTTGATTATTTCAATATTTTTTTCTAAGCATTCTTTTTCTAAGTCATTAATCATGTTTAATTCTTCTTCTAATTTAAGAGCTTCTTGAACATTAGGTTTAGTTACTGGTTCTTTAGGAGCAAAAAGCTCACAGGTTTCATTACATTTAGTAATTGAAAGATCATAGGTTTGAATTTTTTTAGCTAAATTGATTGTTTCAATTTTGTCATTAGTTAATAGTGGCCGCAAAATTGGCATGTTAATATCATGACTAATTGTATAAAGTGATTCTAAAGTTTGTGAAGCTACTTGACCTAAGTTTTCGCCATTAGAAATGGCTAGATTTTTTTTACTTTGAGCTAATTTAGCAGCAATACGATAAAAGCTCCGACGCATAAGAGTAATTTTATAAGCTTGATTTGAAACTAAAGCTATGTAGTTCATTAAAGTAGAATAGTTAGCTAAATAAAAAGTGCTTGTGTTTTGATATTTAGTTAATATTTTGATTAATTCTTTCATTTTATTTACTGTTGCTTGATCAGTTTGAGGAGGTGTAATAAAAGATAAGAAATTAACTTTAAGACCTCTTTTCATAAGTTGAAAAGCAGCAACAGGACTATCAATTCCACCACTCATTAAGTGCAATACTTGCCCACTAACACCAACAGGGAGGCCGCCTAAAGCTTGGACAAAATCACTAAAAACATAAGTAAATTTGCCTCTAACTTCAATATAAACAATAAAGTCTGGATTATTAACATCAACTTTTAAATTTTGGTTGTTTTCTAATAAAAAACCGCCAACTATATTGTTAATTTGACTTGATTGGTATTGAAAACTTTTATTGTTTCTACGAGCAGCAACTTTAAAAGTTCCATTTTTATTTTTTGCTAAATTTAAAGCTTGCTTTTTAATTTCTTCAATATCATTAGCACAAATAATAACAGGTGAGTAAGAAGTAATACCAAAAATGTATTGCAATGCATCTAAATTTTCTTGACTATAAGGCAAAAACATACGGTCAAATTCAACCTCTAATTTATCTTTAGTTTTATGACGAATATTTGAAGCTAAAGTATCAATAAAATTAACTCTATTTTTGCCTTTTAGAGTCAATTCACCATATCTAATTAAAATCTTATTGTACATATTTTTTTCCTTTTTTCATGCAGGTTGCTAGTAGTGAGAAAGCTTCATCAAATTTCTTAGCTCGCATGTGTTTATTGACTGAAATTAAGAGTTCATTAAAGTCTGGATTATTAAATCGATAATGCATGCTTTTTAACATGAGCTCTTCTGTCATTTTCTTATATAAATAAGCCTTAAACGTCATTTTGAATAGCTTTGTAAAGATGTTGTAAAAGTGGTTTCAATTTTTAGATTTGAAGTTAATTTTTTTACTTTGTTCAAAGTCTTTTTTGACTTTTAATAAAGAAACAATTAAAAGCTTAATATCGTTTTCTAATTCTAAACTTTGTTCTAATACTGTGCTTTGAACAAATTGAATTTTAAGGCTTCAAGTTTCCAAAATTTTTAAGTAGTAAGCATTGTAATTTTTTTGATAAGTATCGAGGTTAAAAATCTCAATTTTATCATTGCATTTTTTGATTAATTCCATTATGCCATGAAGTAGTGTTTGCATCTCCATTAAACTAACATTGCCATTAGCTTTATTAGCTTTATTAAAGCTATCATATTGGTTTCTAAGTTGTAACAATTCTTGTGAAATCTTGGTTACAAAATTGCTGTCAAAATCATCTAAATCAATTGAAATATAGCTTAATTTTTGATCAAGCTCATTAATCATTTTGCCTATGTTTTCTAATTCATTATTTATGAAATCTTGAAACTCAGCATTAAGAGCAGTTTTCTTGTTTAAAGTATAATAATCAGCATAGATTTTTTTGATTCTTTCTTGCACATCTTGATAAGGTAATTTTAATCATTCAGTTGCTAAGTTTTCTCTTTCGTCAATTAATTGTTTGATAGCTAATTTGTCTTTTGTTTCAAAAGAGAGCTTGCTTAAGATTTCAGGTAAGTGGTTAAAAAGACTTCATTCGATATTAACAAAGTGGTCCACTTTGCGAGCAAAAATATTGATTCTTTTTTCATGTTGATTTAAAGCATTTGAAATACTATCAATTTTATGAATAAGTTTATTTTCTTCTCATTCAATTGTAGTGTTACTTAATTGTTTTAATTCATTAGTTAAATAATCATAACTATGATTTAAGCAATATTTATTTTTTTCTAAATGTTCTTCAATTTTTCTTAAGATTCTAAATGATTCATTGGTTACTATTTCAATAACATTTCAATGTTTATTTAAGTTCTTAGTTTTTTCATCAAATTGTTTATGCATTGAAATGCATTCTTCATATTTAGGCAAAATTTCTAAGTAAAGTTTTTTAGCTTCTTTAAGTTTATAAGCTTTAGCCGCTTTTCTTAATGCATTATATTTATCATTTAAATCAACAATTATTTTTTTTAATTTGTTGTCTAAACTTTTTAATTGATTTAAAGCAGCTTTATATGTTTCTTGAGTATTTGATAAAGTAATAAAACGTTGAATTGTTGCGTTACGTCCTGTTAGATCATTTTTAATATTTTTAGTTGAATCATCTAAAAATTTAATGTATTTATTAAGATTAGTTTTTCTAATATAAAGGAAAATAAAAATGCCCAAAAGACCTAAACAGGCCAAAATAACTATTGGTACAATAATTTTAATTACTTGCATTTTTCTCCTTAATTAATAATTATATTTTATATTAATATCATTTTTATTTATCAAAGTAGCATTAAAGAATAATAAAAAAATGGGATTGCCCATTTAATTATTATTAAGCATTACGCATATCTAAACTTTTGTAACCTTCAACTTTTTTACCTTTATAAAATCCACAAGATTCACATACACGGTGTTGTTGAATTAATTGAGAACAGTTTTTGCATGCAACAAGATTTTGAACAGGTAAAGCATCATGGGTACGTCTTTTGTGTTTACGTTGTTTAGATGTCTTACGTTTTGGAACTATAGCCACGATTCCTCCTTATACAATTTTTATATTAGCTAATAAATGTATTCTAATTATATGTTAAAAAATTAAAATGTATAGCAATTTATTAAAAATTTTTTTTGTAGTGTAACTAACATTTTATTGCTTTTTAAAGCAATTATTTTCTTGGCCTATTTTACAGTACTTTTAGTTAAAAGTAATATCTTGAAAATAAGTAAAACCATCTAGATTTATTGGTTTTGTGTAGAAGTATTGAACTATTGATTTTTGGTATTCGTTAGTAAAAAGGAAATTATCTTCAGTTACTCTGATTCCTAAAAATGAGTCAATACTTTTTAAAATATTGAATTGTTGATCAAAATTTAAAGCTTGAATTTTTTCTTTAAATCTAGCTTTGAATTCTTTCAATTTCAAATTATTTTTTTGCAAAATTTGATCTAAAAGTGAACTGTTTGCTTTAATTTTTTGCAACTCATAATTTTTAAAGTAATCAACTTTATTAATTGTTTCTTCTATTGTTGAAATAATTTTTTTGATATTTTTATCATAGTTAATTGATTCTAAATTGACTTTTGAATATAAGAATAAATTTAAAAAGAATTGGTTATTTGCTGTTATTAAATTAATTAAATAGTTACTAAATGAATTATCAACTGCACTAAAGTTTTGATATTTAGAAAAGTAATTATTTGCTTCTTTTGAAGCATATTCAAAATTAACATTTAAACGATTGTCTAGGTTGTTGTACAAGAAGGCTATTCTTTTATATAAACTGTCAATAAATGAACTCTTTTCAGCAAAAATAGGTAATGTAAATTGAATTTTTTGATTTTTATAATCTCCATTTTTGCTATAAAAATCATCTAAAATTTGCTTCATTTCATTTTGATAAATTTGATAATTTGCACTCTTTAGTTGTTCATTAATATCCAAAATATTTTTGCTATTTATTGAATGTTTAATTAAGCCATCAGATCCATTAAAATCAATTATTTTATTATCAAATTTATTAGTTTTATAATCAAAATGATAATCATAAACTCGATTAATGTCATCTAAAAAGTTAACATTTTGGTTGTCTAAAATACCACTATTATTGCTGCTGTCATAGTTTAAACTTTGTGAAGCAAATGAATTTCAGTAAGTATTATGATTCAAATTTTTAATAAATGAATATTGATTAATACAGTTATTTAAAATATTTCTGAACACAATTTGATGTGGTTCATAATATTTTTTATTTACTAAATTTTGATCTTTTAATTCATTTAATTTCTGATTATAAAATAGTAAGCTAAAAGCTTCATTAAATTTGAAATCTTTGTTATTTTTAGCATATGGATTAACATTGTAAAAATAGCTTATAACATTACTATTATTTGGTTTAAATAATGTATAAGTAACACCAAAAAGATTAGAGAAATTATCTATTTCATCTTTTTGCAATTGGTTAAAAATATCATAAGAAGCTTCACTTAAAAGATTTTGTTTAAATGCATTATAACTTTGTAATCTAAAGGTAGGCTCATCGTTTTGAATTGGATTATATTTTATAGTCACTTTATTAAGAATATTTTGCTTTTCATTTTGGTTAAAACTATCTAAAGCAGAGTTATTATTTTTAATAAAAATTTGCTCATCAACAGTGTTCTTATTTAAAACATAATAACCTATAAAACAGATGTTTTGGTAATTTTTGCCATAACTATTTTTATTATTTTTATAGTAGTCTGTTGATATTGGATTGAAGATCAAATTATTAGTAATTTCTTGAGTTGTAAATAGTTTAATAAAGTTATTATTTTTGCTTTTTTGAGCATCGTTTAATTCAAAAATTAACTTGTTATTTTGGATGCTATTTTGGCCTAAATTTTTCAAAGAATAGTTGTCTAAAAGAGCTGAAAATTGTTCACGATTTTGTTTCAAATTAAAGCTAGTTAAATAGTCTTGGGCTTGTAATTTATGATTTGTTTTTTTGCCTTTATAATCTGAATAATAAACATCGTCTTTTAATTCAAATTCAATTTTAGTTGCACTATTTAAAGCTTCAAAAAATTCTTTAGAATTTATATTATTATTTTCATCAGAATAAATTTGTTTAATACCGCTTAATGCTCCATCTTCATTAACTTTAGTACTACTTTGAAGAGCTTGATCAACTTTTTCTTTAACATCATCACTATTAAAAATTTTTTGACTATTATCCTTCAGAGTCACTGTTATTGATTTAGCTAAATAGAATTCTAAAAATCTAGTTGTAGTTTTATAAAAGAATTTGTTTAAGTGATCAAATTTACTTTTGCCTAAACTTTTTCATCTAATTAAAGGTGCAGTTAATAAACTCTCAAAATCATTATCAATTCTTTTAACTTTGTTAATAATAAAATTGTCTTTAATTTTGTTTGGTTCATTATATTTTTTAATATAACTATCTTTAGGATTAACTTGTCTAATATTGTTGCATGAAAGAGTAGCGACACTAGAAGTAAGTGTTGCACTAATTAAAAATAAGCTGCCATATTTTTTAATTTTTCTTTTCATACTATCCTTTACTTTTCTAATTTAGGTACACTCGCAATAAAGAAATTTATACCTAAGAATTTTACTAATATTACATAAAAACTTGCTAATATTGAAACAAAAATTGTATATTCAACATTTTTTAGATTGTCAACTATATCTTTAAAAACGTTTCCAATATTTAAGCTTTGATCAATTTTATAAATATTAAAGAAAGCTAATGAAGCTAAAGCTAACATATTAAGTGATAATGAATCAGAAACTAAAGCAATATTTCTTCACAAGCAGTGAATGAAAATAATTTTGTAAATTATTCTTCAATTACTCAAGCCATTAGCTTTGTAAGCTAAAATAAATTCACTGTTTTTGATTTCAAGCCCTATTGAATAACTTGAATAAAAGAAGCTAAGGGAACCAATAAAACCTAAAATTAATATAGCATTAAGATGGTTATATGAACTTATTTTAAAAATCAAGAAAACTACAATAAGGAAAGGCACAATATTAATAGTACTAATTAAATAGTAACTAAATTTAGCACTGCTTTTATTTGAATAATAAGCCACTATTGAACCTAAAAAGGTCCCTAAAAATAATTGGATTAAAGCAGCCACAAATGTTACTAAAATACTTATGCCAAATGAATGAATAAAGAAACTAAAGCGGTCTATACCTTGAGAATTGGTACCATATAAAAAGATATAGTTAGTGCCTTTTAAGGCATAAAGCAATTGATATGGATTGTAAGTTAAATTAACTAAGTCAAAATCAGGTGTTTCATTTATGACTATTTTTAAATTAGGATGATCTTTTTGAGTTTGCAAAATAAATTGATATAAGGGGTCACTTTGTGCAAACTTTTTAGTAATACTTGCATTTAAGTAGTTAGGCAAATTATAAGCAAGTTGTGAATCGATGACACTAGTTTTTGGCGAATAAGGGCTAAAAATAGCACTTAAAGTCAAAAACAGTAAGAAGATTCCAATAATTGAAACTAAAGCAATGTTGATCTTTTTGCTAAAGAAACGAATTAAGTATTGTTTAAATTCACTTTTGTTATTGCTGATATTTACTTGGAAGTGTTTATGGTGAGTTAAAGTGAAAAGACTTTTTTCAAATTCAATATGACTTTGGCCTGCTTTTTCGTGAGAATGGTGGTGCTCATGATTAAATGCCATTATGCGCCTCCTTTCTGAATCTAGCTTTTTCTCTATTTTCTTTGAAGTTAAAATTGAAATTAAAATTATAACCATTTTCAACTTGCAATAAATCATAAGTTAATTCACAAATAAAAGCTAATAAGAATAAAAGCAAAGCTTTAAAAAATACTAAAGCCATAATAACATTAATTTCTTTTTGATCAAACATGCTAATTAAAATAACACTTTGACCTGGTATTTGAAAAATACGTTCGATAATTAAGCTAAAACTTATAGTTAAAACTAAAATTGGCACTATTTGGTTAACTAATGAAATAAATAAATTTTTAATAACAGCTTTATTAAATATTTGTAAAGTACTTAAGCCATTTGCTTTTAAAGTGATTACATAATCTTTTTTTAGAATTTGAACAAGATTATTTTTGGTAATGGTTGCAAAGAATGCAATGATTCCTAAAGATAAAAGTGAAATAGGAAGTATTAAAGAAAGCAATGTATAACCAAAACCAAAAGCTGATGGCTCTATAAAATTAACTGGTAAGTCATTGACTTCTGAAATAATTAGCATTATTGGTGCAAAGATAAAAGTAGGAATAGCAGCTAAAATAAAGACAATAAATGAGAGGACTAAATCAGTCATTTTATTGTTTTTATAGCCTAATCATATTCCGACAAAAACACCTATAAAAAAGCTAAATACAAAAGAAATGCTTACAAAAAGTAAACTAAATTTATAATAATGGAAGAATAATTCTAACGATGAATTGAAACCACTATTATTAGTTGAAAATGGTTTACCAAAACCACTAAAAATAGAACCTAAGAAAGTGAAATAATTTTTGATAATTGGTTCTCTGTTTATTTGTGAGTTATTAATAAATAAAGCTATAACAAAATAGCCAAGGGTAACTGTTATAAAAATACCTAAGAATGTAAGTAGAATTTTTTTGAAAAAGTATTTGCTAAAATTCATAAAAATTCATCTTTCTACATAAAAAAACCGCTTAATGGCGGTTTCTTATTACATTAAATAATTTCATTTGATTTAAAAGCATCATAAATAGCTTTGTATGCTTCTTCTTCATCTGCTCCATTGATTTTAATAGTAATTACGTCGCCTTTTTTAACACCTAAAGACATAACATTCATAATTGATTTAAGATTTCCAACTTTTCCTGTCTTGTCTAAAACAATTTGCACATCACTCTTGAATTTTGAAGCAATTGTTGAAACAACACTAGCAGGTCTTGCGTGAAGACCAATTGGATCTATAATTGAAGCTGTAAATTCTTTCATGTTTATCTCCTTATTAATTTAATATTTCAAAATATATCTTCTTAAATATCTAAAGTTGCCAATAAATATTATTAATATTATATACTAATTCTTATATATACTTATAATATTACTACAAATGTCATTTTTAAAATATAAATTTTAAAGAATTTTTTAGTGGTGTTTTTAACTTATAAACCTTTAAAACAGTTTGTTTGGTAAATTATTCTGCTTTTTCAACTTTGAAAAACTTTTCGTTTAAGGTTTGAATATTTTTTCCAATGTTTTCAAAATCGATATTACTACTAATTAATGCTTTTGTTATGTTTGTGATTTTATTGAAAGGTAAATCAACAGCAATACCCGTTTCTCGACATTGATATTCTTTGTTTGATGAAACTAGTTTTGCTTTTTCTAAGGCTTTAATAGTTTCATTAATTTGTTCAGTTTTAATAAATACTTCAAAGTTTGAATTTTTTAAAATTTCTAAGTCACCAGAATATAAAACTTTACCTTTATCTAAAACAGTTAGACTATCAATATATTGTTCTAGCTCACTTAAAATATGAGAAGAAACAAAAATGGTTTTGCCTTGTTTGTGAAGTTTGCTTAAGTTAAAATAAAGTTCATTTCTAACTTTTGGATCTAAGTTTGCTGCAGGCTCATCTAAGATCAATATTTCTGGATTATGAATTAAAGCTTGGACTAAAATAACTTTCTTTTGTTGACCTGAAGATAAATTTTTACCTATCTTTTTTCATATTTTTTTGTTAACATCTAACTCGATTAATAATTCTTCAATTCTTTTTTTAGCTTCGTCATTTTTAAGTCCATTTAATTCAGCTAAAGCAACTAAGTAATCATAAATATTCAGACTTTTAGGAAATGTTGCTACTTCAGGAACATAACCAATTTTGATTCTACTTTTTTTGTTTTTAAAGTTAATTCCATCTATTAAAATTTCACCTTGGACTTCTGGATAAAAATACAATAATGAACGGAAACTTGTAGTTTTACCTGCTCCATTTGCTCCAATAAATCCATGAAATTCACCTTTTTTGATTGAAAATGTCACATTGTCTAAAGCTTTAAAATTTTTAAAACTCATTGTAACATTTTTAAATTCAATTGCGTTTTGTGACATTTTTTCCTCCTATTTTAAAAAGTTAGTTTTTTTGTAAATAAATCATCCGCCAATAGCTAAAGGTGTAGCCATTAAAAAGTAAATAATTATTTGCATGTAAGATGGAAATATATTAACTTTTTGTACTTTGTAAAATTTCAAATCAATGTTGTTTTCTTTTAATAAATCTTTATTAATTAGCATGCTGCCAAAAATTTCTTCAAGTCTTGAATTAAAGAATTCTTTATTTTGATTTATTTTTAAAGTTTCGCGATAATCATTCATAATAACTTTTGACATTGCATCAACTATAGAATATAAATTATTTTTTACCAAGATTGTAAAGAAAAGTTTTTTTGCAATAGAGTTATAGTCAAAGCTTAAGTAACGAGTTTTTTTAAATAATTCTTCTTTTTTGTCAAAAGTTAAATTATCTTCAACAAAATTTCTGAATTCTCTTTCTTCATTTTTTGCTTTTGAAGGATCTTCATTAGCTGAGAGAAAAATGGTACTTCTATATATTAATGGCACCATTTCATTGTAAAGTCTTTGAGTTGCAAAATTAATTAATGTTTCATATGTTTCAAGAGTTTTGGTTGAGTCTTTACCATCAAAATTTGCTTTAGCATTGACAATTTCAGTTTTGAAATCGGTTTTTAAATTTTCAATTACATTAACAAGACCATTAAATAATTTTTGACTAAAAACATCTGGATTTGCTTTTAAAATTTGGGCTTCTTTTTCAAAACGATAAACTCTATCAGTATATTCATCATTTCATTTTATAATGTGTTTTTTGCGAGCCATATAAGTAATTTTTTGTTCTAAGGTTTCTTGTTTTTGATATGGATTAAGTCGATCTGTTGTGCTTAAATCATAATCAATAATATAAATCTTTTGATTTCCATTTGTGACATAATCATAATTATTCAAAACAGATTCCATATCTCTTTTATAGAAATATTGGTTATGAACTCCTTCACTCATAATTGGAATAAAGCGGTTGCTCTTTGGATCATCTAAAATAAAATCATTATAAAAGTTCATTGATCAAAAGTTCATATTTAAACCATTAGATAAATTGACAACTGAAAAATTATGTTTAAATAAATTTGAGCTTGAAGTTACATAATCATTAATAACAAATTTTTTGTTTATTTCATTTTCTGCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAAGTTAAGCCTTCATTTTGAGGCATCTTAAAAATATAAGAACCCGTAATAATTGCGGCCAATATAATTAAAATAGGTGACATGCTTCCATAATAAATAACTATATCTCGAGCTGAATTTGTGTGAGATACAATAAGAATAATAAGGGTACCTATTAAAAGTAAAAACGGTACTAGGAAAAAGTTAATAATTAATATTGACTTGTCTTTGTATGCTGTTTTAAAGTTTAACTTTAATAATTTTCCCATTTTTTCCTTTCATATTTGTATGCTACTATTTTACTAAATTATTAATAAAATAGTCTAATTTGGAAAAAATAACCACAAATTTGAATAAAAAAATGTAGCTTTCTTGCTACACTAATTATTAGAATGAAAATTACTACTATTTTCTTAAGTTTAATTCAGCTAAAACTTTGTTGTATGTTTCTAAGTCATTTGACTTAAGGTGTTGTAATAAAACACGACGTTTTGAAATTTTTGCTAAGAAACCACGACGTGAGTGGTTGTCTTTAGGATTTGCTGCAAAGTGTTTTTTCAAATCTTCAATTTCTGCTGTTAAAATTGCGATTTGAACAAATGTGTTTCCTGTGTCTTTTTTATTAGCACCATACTTAGCAACTAATTTAGCTTTTTCTTCTTTTGTAATCATATATCGTCTCCTTATATATGTTCTAACCTAGCTAAAATCTAATATCTTAACCAAGTATAAAATTTGAATGCATTTTTTAATGCCTATAAATAATAGCACATTAATAATAAAAGCAAAATAAAAACATTTTGTGACCTAATCGTATTATTTTCAAAAGTTAGATTTTTAAAACCTGGATGTTTCACTTTTGGAATTAACAATAATTAGTAAAAAATATTAAAAAATTTTTGAAACACACGGCAAAGTTGTTATATAATAGAAATCACTATATTTCATTTATAGTATTTAGGACATTTATTAAATAAGAGAAAGGAAACAAAAATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAGCCTACTAATATAGAAGGAGAAAATTATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGATTAGTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGATAATATAAGATATTATGGCAAGACAATATAAATTAGAAGATTACCTTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGAATGGCCCAAGAACAAGAACGTGGTATTACTATTACTTCAGCTGCTACAACAGCATTCTGAAAAGGAAAAAGAATTAACATTATCGACACCCCAGGACACGTTGACTTTACAGTTGAAGTTGAACGTTCACTTCGTGTATTAGACGGTGCTGTTACCGTTTTAGATGCTCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCTACAAACTACAAAGTTCCTCGTTTAGTTTATGTAAACAAAATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCGGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGAATCAAATTTGAACCAAACCATGACAAAGGTTTTGAATTCGTAGACAAAATTGTTGGTGGTAAAATTCCTAAAGAATACATTAAACCAATTCAAAAAGGCCTTGAAGACAAAATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAATAATACAAAAAATGAACTCATAAACAAAAAACCGAGTTCATTTTTTTATATCATAGATATAAGGCAAATTAGAATAATCTAAAATGTATATTTTTCCAGAATTATGAATAATTTGATTAATAAAAGGAGTAAGTTATGACAGATAAACTTTTTGAAAAAAGCAATATTTGCCTAGACTTGTTGACCAAAAAATAAAAGATTCCTTAGAAGTTATTGGAGGAATATTTTTGGTTGGCCCTAAATATTGTGGCAAAACATGAACAGGAACTAATTTTACTAAAAGTCAAATAAACATACTAAAAAACAATTTAGACTATGTATTTGATTTAAACAATACCCTTCAAGGAAAAACACCTCGACTTATAGATGAGTGACAAATTAGACCAGAAATTTGAAATGCTGTAGTCAATGAAATTAGTGTTAGAAGAATATATTTTAACTGGATCAATACTAAGTAACTGAGACAAGATACAACACTCTAACGTTGATCGTATCGGAATGTTAAATATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGAAAAGATGGATGACCTTCTTTAATTAACAATAACTCAAATGCTCTAGTTCAAATTTGATTTGATTACTATATTAACAATATAATGAATCTTGATGAATAATATTTTAAAGCTAGAAAAATAGACTTTGACAAATACAATTTATTTCTTAAATCACTAGCAAGAAATGAAGGTCAAACAATCAAATTAAGTGCTATTGCAAATTATATAAATAATGTTGCTAAAATTGATACAAGAACAATTGATGATTATTTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGAAATATTTTACATTATAGAGATAAATGGTCAAATGAGGTAGACTGTATTTTGCAACTTAAAAATGGAGACTTTGCAGCCATTAAAATTAAATTAAGTAATAGTTATGAAGAGCAAACAGCTACAGGCTTACTTAGGTTTAAAAAAGAAATGGAAGAAGAACAAAAATTAGCTAAAATCAATAGGGAAAAAATGTACAAAAAAGTACCTAAATGATTAATAATAATTAGTGGGACAGCTCAAAAGGCATATCAAAGAGAAGATAAAATTTTTGTAATTCCATATAAAATGCTTTGTGAATAATTTTGCTTTATAAGCCTATAAAACAGCCAAAAATAAAAAAGACCATTATCTAAAAATGATCTTTATTCCTTTGGCTTTTGATTCATTAGAAACTATGTCCATAATTTCTTTTTTAATTCTTGGATCTAGATTAATTTTTTCACTTTCAAAATAATTTTTGAACATTGTACGATCATTATGAAAAAGAAAAGTCATATAATAAATTTCAGCTGTTACTTCAGGGAATTTTATTAACTTTTTCAAGATTAGAAATGATTCAACTGTAATACTTAAATAAACAATTACAACTATCATACAAAATGCTGCTGCAGTCGCCGCTAAATCTTTAGTTTGCTTCATTATATTATTGTCATATTTATACTCTAAACCTTTGTAAATTGGCATAATAAAGATGCACAAATAAAAGATAAAAGCCATTAAACATGAAGCAATAATGTTATATTTTGAAACGCCTTTACGATCAAATTTAATTCAAAAATAACAAGCTAAAATAAGCGTAATTAATGAAATTATTGAACTAGTTGCAATATAAACAAAAAAATTATATCAATAATTTAAACCACTTAAATAGTAATCATAATTCTTTAATGCTAAACTCAAAAAAGTTACAGTAATAGTAAAAGCTACTAATAAAGAAATAAAAGCAAAAAGAGAACTTCAAGACAAATCTCTTCTTAATTTAACTTCTTCTGTGTGATATTTTTGAGCTTCTTGATTAAAAGTTTCAAGCATTTTGTATACCTCTTAACTAATTTTATAAAAAATAATTTTTTTTAATTGAATTTAATTTATTTTAGTGTATTCTTATATTAATTAGAAGCCAAAAATAGGCTTCAAAATAATAAATATAAAGCATAAAGAGTTGCTGAGCTAGCTGGCTTTGACGCATGCCAACCTGCATTTGTAAGGTGGACAGTGAGGGTTTATTAACTAAACTAACATGTTTAATTATTGAGCTAATAACCTCTAACTTTTTATGCAACAAAAGTGGAGGTTTTTTATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGACCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGAGAAAAATTAGACAAAGTTGAAGCTATCAAAAAATTAATGAAATAAATTATTTCAAAATTAAAAAATAATGTAAAATAAATACACTTATATTTTTGATTTTAATACACTAAGGAGGAACATCTTATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAATAATCATAATAATAAGCAAGGAACAATTTTGTTCTTTGTTTTTTTATCCTTTTTTAGGAAGATATTATTAATTTTGAATTAAGTTTTTTAAACTTGGATGGTTCTCACTTTTATAACTTTTAGAATTGTGGTTCAAAATATAATACCTATTGATTTACTTGGAAATCTGAATATTGAAATTCAGGTACAAGATATTAAAACTAATGAAACATCTAAAGTAAGAAAATTTAATTTACTTTTTAAAAGTTTGCTAAATAAAAATAAAAGAGCCTTATAAAAAGGCTCAAATATTATTAATTAAGTATGAAATTATTTAACTTCAACTGTAGCACCAGCTTCAACAAGTTTAGCTTTGATAGCTTCTGCTTCATCTGGTTTGATGTTTTCTTTAACAACAGCTGGAAGGTTTGAAACTAATTTGTTTGCATCCATTAAAGCTAAGTTTAATAATTCTTTAACTGCTTTAACAATAGGTAATTTTTTACCATTGTCTGAAGTAATTGAAACTGTAACTACTGAAGGACCTTCAGCAGCTGCAGCTGCAGCAGGAGCAGCAGCAACAGCAACAGCTGATGGGTCAATTCCAAATTCTTCTTTCATTGCTTCAACTAATTCCATAACTTCTTTAATTGACATTTCTTTTAATGATGAAATGAATGATTCTTTTGTTAATTTTGCCATGATTAATTTCCTTTCGTAAAATTATTTGTTTTCTTCTTTTTCACTAAACATTTTTAGAGAAAGAGAGAGTTGTTGTAATGGTGACATAAGTGAACGAGCAAGAATTGTAAGAGCTTCTTCATAGTTTGGTAATGAAGCAACTTCTTGAACACCTTTAGCATCGATAACTTTGCCTTCAAAAATACCAGCTTTAGGAACCAAAAGTTTGTTTTCTTTAGAGAATTTAACTAAAACTTTTGCAGCAATTAGAGCATCGGTTGTACCAAAAGCAAAAATGTTTGGTCCTACTAAGTGTTGATCTAAATCATTAATGCCATGATTTTTAGCAGCTATTTTGAAAAGACGGTTTTTGTAAACTTTAATGTCAATACCTTCTTTTTTAGCTTCTTGACGTAATGAAGTCAATTCAGCAACACTTAATCCACGATATTCAGCTATAACAAGAGCTTGTGAAGATTCAAGTTTATTTTGAATTTCTTTAACTGTATCTTTTTTAACTAATCTAAAAGCAGATTCATGTCTTTTTTGCATGAGTATCCCCCTTTCATAATTTAATATGCAACACGGGACAATAATAATGTGATAGAAAAAAAGTAATTCTACACTCGGTAACGAATTAAGCTTTCGCTGTTACTGTCTTTATGTATTGCGTTTTTATTATATTAATAAATTTAAAAAATCAAAGCAATTTTTTTACTAATTTTAGCCAGTTGATTTTAATAGAAAAAATAAAAACAGCAAGTAAGCTGCTTTATTAATTTTTTTCATTATCAGTTAAGGTTTCATCAGTATTTAAAATGTCTGCTTTATTACCTAATTCAATATCTACACGGACATTTTTGAATCTGTTAGTTCCTAAGTCTATAACTTTAAAGTAAACATCATTGTATTCATATTTTGTATTTTTATAAAGTTTGTGGCCGAGTCTTTTTTCTAATCATTTATGAAGTGTTAATTCATTTTCTTCTTCTTCAAGATCTAATTCTATTTCTAGTTTTTTAATTAATTCTTTCATTGTTAAAGAAGCTGAGACATGGAAGTGTTCAAGACTAATTTCAAAAATGTCTTTTAATTCTTCATCATCAAATTCATCATAAATTTCGCCAACAATTTCTTCAATAATGTCTTCAATTGTAATAATTCCAATTATTTCACTTGAGTTGTTATTTGGTGTAACAAAAGCCATTTGAGCTCTTTGGTACCTCATTTTTTCTAGAGCCGAAGAAAGAGAAGCATTAGCACTAATAGTAGGCACAGTTTTAAAATAGTTTATAACTTTACCTTTTTGTAAAAAGAAGACATCTTTTAAGTGTAAAATGCCAATTAAATTTTCATCTTTTTTAACAGGAATACGGCTATAGTTTGTATCTTTAAAAATTTCAAATGCTTCTTGAATATTAGCATTAGCATCAATTATAGATACATCTTTTAATCTTATGTAGTGTTTTTTAACTTTGGTACTATCTAAGTCTAAAGCATTTTGAGCCATGATGCTTTCATTAGCCTCTAAAACGCCTTCGTTTTGCGCAATATCAATAATATTTTTAACATCATTTTCTGTATTTGTTATATAGATTTTTTTACCAATCTTGCTTATTGGATAGGTAATTGGTCAAAACAAGTAATATAAAATAATTAACAAGTAGCAGAAAACTTTAACTGTCGCTTCAGGTTTACTTTTGGCTATTAATTTAGGTGTAATTTCTCCAAATAAAACAATTATTGGAGTCATTACAGCAGTTGAAATTAAAACGTTATATGATTCGAATTTTTTACCTAAGTAAGTTGATAAGAGTCAGGATGTTAAGGCCGAAGCAGCAATATTAACTATATTGTTGCATATTAAAATAGTACTTAAAGTTTGGTTGAAAAATGTATGTTGTTTTTTGATTATTTTGTGGCCAAATTCTTTATTGCCAACCATTGTTTCAATTTTGCCAACATTTAAGGATGTATAAGCAGTTTCACTGGCACTAAAAATTCCGCTAGCTATTAACAAAATAACTAGTCCGAATATGCTTAATCCTAATTTCAGTTTCGGGTCCATGATTATTCCTCTTCTTCGTCATCATCACTTGAGACGGCAATTTGTTCATTTTCAAAATTAATGAAACGACCACATGACATTTTGAATCATAATGGAAAAATTCCTGTTGGTCCATTTCTGTGTTTTGCAATAATGATATCTATTTTTTCTCCAATATCTCCAAAGAGATCTTTGGTTTCTTGTGAGACTTGTTTTTTATAATAATTTTCACGATATAAGAACATAACAATATCGGCATCTTGTTCAATGTTACCTGATTCACGTAAGTCAGACATAATAGGTCTTTTGTCTTCACGTTTTTCAACTTCACGGGAAAGTTGGCTTAGTGCAATAATTGGAACATTTAATTCTAAGGCTAATGTTTTTAATGAACGGCTAATTTTAGCTATTTCATTTTGCCGACTTTCATTTCTTCTATTTGAGTCTGATGAAATCAATTGTAAGTAGTCAATAACAATTAAATCAATTGGACTAACTTTGTGTAAACGACGACATTTTCAAAGCAGTGTATTTAATTCATTATTTACTGATTCATCTATAAATAAGTTCATTTTGTCAATTACATCTTGTTTTAAACTGTTGATTAAAAGAAATTCATTATCTGTAATTGTTTGAGGTTGTTTTAGTTTTTGAAGTGGCACATTTGTGTTCAAGCTATAAATTCTACCCATAAGTTGGTTTGATGACATTTCAAGCGTAAAGAAGACAGCTCTTCTGTTTTTTCTTTTAGCAACATTTGAAGCAATATTAAGAGCAAAAGCAGTTTTACCCATTGCTGGACGAGCAGCAATAATAATTAATTCACCACCATGTAGACCTTGAGTAATTTGGTCTAAATTGTCGTAACCTGTTGCTAGTCCATTTAATTCATTTAGATCTCTTGAGTGGCGATTTTCTAAATCTTTAAAGTATTCATCGCTAACTTCTTTGGCTGTTAAAAAGTCTGCACTAACTTGACTACGATCAATGTCAAGTAATAGGTTTTGTAAATCTGAAATAACATCTTTTTCATTGATTGCTTTTTCAACATCAAGTCTTCTTTGAACTAAAGCTAAACGTGATTCAATTAAACGTAATTTTGTTAACTTAACTAATTCTTCTAAGTAAGTTTGAATATTAGATGAGAAACCAGCAGTATGATAAAGTTTAGATAAATATAAAGGTGTTATTTGAACAAAGTTTCTTTTTTTAGCAACAGTAAAAATGTTTTCTTCATTAATCGAAATGTTGTTTTCAAATAGTTCTTTAAAAACAGAGAAAAGTCTTTGATTTTCTAAAATTGCAAAGTCTTCTTCAAGTAAGTAAGGCACAATTTTAGGTGCGTTTTTGTTGTCTGTTAAGATTAAGCCTAAAAGAGACTCCTCATATCTAGAGTTAATATGACGAGATGTATTTATTGGTGCATTACTTGGATTATTATTTGGCATTAATTATCACTTCTACTTTCAATTTTGCAATGATATCTTTATATAAAATAACATCTATATCATGCAATCCATTAGATACTAAATGAACTTTTTGAACTGCGTATTTATCTAATTTATAGCCATTTTGGACTAAATCTTTAACAATATCTTTGGTTGATACAGCACCATGAGCAATTAAATTTCCATTATTGTCAATATGCGCATCTAAAGTATATTTTAAAGTTAATTTTTCAATTTGATCTTTAAGTTCAAGAGCTTCTGAACGTTTTTCCATTTCATTTTTAGTAAGCTCACTAAGTCTTTGTTCAAGCTCTTTTTTAGTCTTGTCATTATAGACAACTGCAAAACCTTTTGCAATTAAGAAGTTATTACCATAACCAGCTGAAACTTCAATAATTGTATTGGCTTTACCTTTATCACAATCTTTAATCAATATTACTTTCATTTTTCACACTCACTATCGCTTGTTTAATATTATCTACAAATGTCAACAAGTCTTCTTTTGAAACTGCAGCTGCTGTCCCAAAGTGTCCACCTCCACCAACAGCTTCGCAAATAATTTGAACATTTGTATCAATACCTCTGGCACTTAATTTATATGATTTTTTGTCTTCACTTTTAGCTACAACAAAACTTGCTTTACGACCACTAATTCTAAGAATTTCTTCAGCTGCTATAGAAATAACATCATTCGAAAGTGGAATATCTTTGTAAGCTAAATAGAATCCTGGTTTTACTTCTTCTAAGTTTTTTAATACTTGATCAACTTTTTCATAAGTGGTTGCATCAATTTTTAATGAATTAGAACTTTCAGTTGAATCTGCGCCTCTATTTTTAAGTCAACTTGCTGCTTCAAATGTTCTTGAAGTAACATGTTTTTGAAATTGTAAGGTGTCAAGATAAATACCATTTAATAACATTTGAGCAATGTTCTTTTTAACTTTAACTTGCTTTTCTAAAAACATCATTAATTCAGTTACTATTTCACTAGCACTTGAAGCTGAAGGCTCAATGTGGCGATTAATTTTTGAACAAAAGTCAACTGAAGAATTTAATCTATGGTGGTCTAAAATAAAGATATTTGTGTTTTTAGCTTTTTCAACACATTTTGGATTATCAGTCCTATTTGGAGCTGAGTTATCAACTAAAAATACAAGTGTATTTTCATCAGTCAATGTGTTAGCTTGATGAGCTTTAATGAAAATTGGATTATCTTCACCAAAGTATTTAGCAATTGCTTTTTTAGTTGTAACATCTTGAGTCATTGTGCAAATGTAAGCTTCTTTATCAAATGTTTTAGCTATAGCATAAACTCCTAAAGCTGAACCGATTGCATCCAAGTCTGCATGTGCATGACCATAAGCAATAACTCTTTTAATTTTTGGATCTAAAAGTTTCTTTTCAATTCAATTAGCAAGTGCTTTGATTCTTGCACGGTTGATATTTGGCAAGATTTCACTTGTTGAACCAAAGTAACGAGGTAAATTATTTCTTGAGAAAATAGCTACTTGGTCACCACCACGACTTTGAGCTTGTAATAAAGCATTTTTAGCTTGTTCCATTTTTTCTCATAAGTTTTTTGTTCCATATGCAAAACCTGCAGAAACTGAAATAATAATGTTTTGACTTTTTAGTGTTTTATGTAAATCATCAAAAAACTTAAAGTTTACTTGACTCATTTTATCTAAAGCTTGCTTGTTAGTTACAATAAGAAATTTACCGTTTGTATTATATTGACGATAAACTAAGTTGTAATCACACACTAAGTTATCTAATACTGTAACAACTTCTTTAGTTACATTATAAAGTTGTTCTTCAGAAAGAATTGATTGATAAAGCTGATAGTTATCAATTTCAATTTCACCTAAAACTGTTAATTCATCTTCATATAATTGAAGAGTTCTTTGTTCAAGAGTATTGTCCTTAATACTTAAACAATTTTCAAGTGGTCAAATATTTACTGTATATGAAAAATCTTTGTAATTAAAATCAAAAGAAATGTTATTAGAATCAAAATTAATATTAAATTTCTTGAAAAAATCAGTTAATTTTGATCCGACTCATTTTCTACCAAAACGGTTTTTAATAAATTTACTTGTTCAAAGAATTTGGCCTTCACTGTCAAAAATAAGCACTCCAATTGCATTATGACTAATGATTTCATCAATGAAACTGTTAAAAGATTTTTTTACTATAGCTTGAGTTGATAAAAAACTTTTAACAGATAAAATTCCAAATAATCCAACGATTATTAATGAAAGACATATGCCAAAAATTCCAAAAATGCTGCTATAAATATCTGAACGAGTAAAATACAAAATTACTGACCCTACAGCTAGGAAAACGCCAGCAACTGCTATTAAAGAATATAAAATTATTTTTTGTTTTAAGTTCATAATATAAATTATATATTAAGTATTAATATTCATTTGTAATATATAAAATTTTTGACTTAAAATAAAAATAATCATGAAAAAAACAACAAAAAACTAAAAATGCTATTTTATAGTTCTTTTACTTTTAATTATATTTCTTTTCAATGTTTGAAAGCAAGCTTTTTATGTCTTGCATTTTGTCTTTAAATGTTTCTTGAGTTTCTTCAAATTTGTATTCATTCAAAATAAATTGATAGTCATCTTTGTCAATTTTTAAGAATTTTTTTTCTTACTCATTTTTTTGATAACTACAAATTTTAGTATTTTATCATCTGTTGTTTTTCTAAATTCATCTATAAAAAATTGGAGTTTGTTTTCAATATTTAAATCAAGCATTTCATTAAAGGAATTTATAAGTTTTTTAGAATCATTATTATTGTGGTCAATATTAGCCAAAAGCAGAAAATCAGATTTTGATCTAGAAATAACAAAATGATATTCATAATCAGAGTCATCAAAATTGTAATTAGTTCGAAAAACACCTTTGATGTATTTTTCTTTATTTAAAAATTCAACAATGATTTGTATTTTTTTATTATTTATTAAATTATTTTTTGATATAGAAATTATTAATTTATCATATTCAAGATTTTGTAAAAAGTAATCATTATTTTTTAAGATATTTCCATTATTATCAAATACTAAAAAGTCAAAAGAATTAGCAGTTTCTTTTTCAATTAATAATGGGTTATTGAAGTCGATTCAGGTTATAATTTGACCTGTGTTTGCATTTAAATTTTTATTTAATATTTCATTTAGGGAGCTTTTTTCAAAGATATAGCGTTTGTCTTTTTCTCTAAAAATATACTGTGAATTGTAAGTGGTTAATTTATTATTAACTAGATAAAGCATTATGTTTTTCATTCTATTAATTAAGAAATTTAATTTATTATTTTCATGTCAAGTATTCATATTTTCAAGATGTCTTTTATTTAAAATTATTTTATAAAGATTTGAGTTTTGCATATAGAACAAATTGTCGTTATTAACTGTAAAATAGGTTTTTTTGTAAAAAGTGTTATCTTTTGGAAAAACATTTGTTAAGCTATCTATAAAGTTTAAACAATCTGAAAAATTGTTATAAAATTCTAGATTTGCTTCAAATTTTTCCAATCTTTTTCAATACAATTCATAACTAGAAACAAAGTTATTAAAAGCAAGAACAAAGTTGGTATGAACCTTAAATATTGGACTTTTAATTATGTTTGGACTTATTCCTGTATTGAACAGGGCTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTTCTAAAGCGGTTTTAAATTTATTTTCTTCCCCAAATCAAAATTCTCTTTTTACAACACTAAATAAACATTCAGCGGGACTATTGTCTAAACAATTTCCTTTTCTTAACAAGCTTTGAATTGTTTGTTTCTTTTTCAAATAATTTATATACTCTTCATGAGTATATTGTCATCCTTGATAAGAATGCAATAATACATTGTTTAAGCTATGTCAATTCTCTTCTACGTTTTCCAACATTTTACCCGCCATTCTTAAGTTAGTTGATTTAGAAATCGAATAACCTAAAATCTCTCAACTTTTAAAATCAACCACACGAAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTATTCCTAGTATTTTCAAATATTTATCACTAAAAAAATTTTTTTGTATCTGAAGAAATATTTTGCGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTTTGTGAGTATTGCCTATGAATGAAGAATACTTATTTTTTTCTTAATGTTTTGCCAAAAAGATTTAATGATTTTATTAATTTTTGAACCTTTTTATGATTAATCATTATTCCTTTATTTCTTAATGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAATATAATCTTTTTCAAATTTTCATCTTTGTCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAATTGACTTTTTCTAATATTAATTTAATAGAAAAATAACTTTTTAAATTCTCGCCTTAGTTCAATTATTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAAACGCTCACTTTTTTTGCTATCGCGTTTTCCAATTTAAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATCTTCCATTTTATTTTCAATATTGATTTTAAAATTTGTTATTTTTTTAAATTTAAATTGAATAAAAATGAGCCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAAATGACAAAATTAAAAACATTTTTTAAATATTTTAGATGAATGCAAATTTATCAATTATATAAATGTTGGATATGACTGTAGTAAATTAATTTTAATTTTTCAAAGTAATGTTAGTCAAGATAATTATTCAAATAACAAAGATAAAAATAGCCAAAAAGAACAAGAAAAAATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGAAAAAGCTATTTCAAATTTATTTATCGATATTAAGAAATCAGATAAATTTTATGATGATGAATTGAAGCTTAAAATTTATGCTATTAAAAATGATGGTAGTGGCAAAGTATTCAGAACCAAAATTTACTAATTTTAAGAATTTTTCAGAGCTTATTTATAAAAAATAGGCTCTTTTTTATTTGCAAATAATATCCACTATTTTTTGTTGTATAATACAAAAACTATGGATCAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGACAAAGAACATTCAAAAAAGATACAGCTAATAAAATTCAAAAATTTGCTGAAGAAAATAAGATTTTATATGTAATTTTCGATGAAGAAGGCGAAACAATTTATGTCCATAATGCTTTTGATTATTGAATCAATAAATTGTTTCTTTCTAAAAAAATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCAAAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGCTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTGGGCGCTCCTCAGAATGCTAAAAGAGTTGATACAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAGTTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGAGATGCAGGTTATAATATTAAAAATTATCAAGAATTTAAGAGTGAAATTAATAAATATAATTTACTAAAACATATAAAAGTTATTCATTTAAATGATTCAAAAAATGATTTAAATTCACATAAAGATCGCCATGAAAATATCGATAAAGGTTTTATTGGAATTGATACTTTAGCTAAGTTTGTTCATGATAAAGATTTTGATAATGTGCCAATAATTTTAGAAACTCCAATTCCTGAAAATGGACCTATTTATGATCAAGAAATAGCTGCTTTATTAGCTTACAAAAAATAACAAAAACACCGAGAAATTCTCAGTGTAATTTTGTGCTTTTTTACCGTATTTTTACCATGAATAGCTTTTGCATTATTTAATGTACTATTTGCAATTTCATTAATTAATTTATAGTTTTCATGATCACTAGTTTGATGAATTTAAATTAATTCATTTAACTTTTGAATTTCTCCATTCAATCATTCCTTAGTTTTTTTAATTTCATCACTTTCAATATTAGGCATTTCAGGTTTCTAAGCGCATCCTGTTGATATTGCTATTGTTGGTAAAATTGTTGTTGAAATAGAAATAGCTACAACAAAAAATGTTTATTTTTTTATAATAATTTGCCTCTATTTTTTTCTATTATCACAATATAAAATGTATAAAAATTTAATTCTTTTCATAATAATATGAAACAGTTTTTGCAACAATTTATTGAAAAAATTACCATATTAATAAAACTAAAAAAATTAAAAATAATGAAATATTGAAATGTTTTATTTACTAATAAAAAGGTTACACATTAATAATAAATACTAAATTAGATATTAATATTTATATAAAAATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGATTTGGTTCAATATTCAAAAATCATTATTTTGCTTGGTTTAAATCTTATACAAGAGTTGATTACTGAATTTTAAGAAAAAGAATCATCAGCTTTGTTTGGTTAACATTATTATTTGTAGTTTTAATTTATCATGTGGTTTTAGTTAGCTTCCGTGTTGAAAACATTTGATATTATTCAACAGCTGATGTTAAAAATATTTACTTAAAAGGTTGATACTATACTTTTACAAATCAAGCTGATTTATTAGATGGTAAAAGTGTTGATTATCCAGTTGCTTATTTAAATATTGGTTTTTTACTTGATACATTATTTAATCTTTTTTATGTAGTAACTTTTAGTCCTTATTTAGCAATAATTAGTGCATTTTTAATTTGAACCTTAGCTTGATTATTCCTTGTAACATTAAATCCAATTAAATATTTTAAAAACATTAACGAACACAAAAAAACAATTGTTATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGAGTTAGTCCAATATTAAGTGATAATTAGGAGTAAAAATGAAACAAGTTGCATGAAATGAAGTACAAAATATGGTTGCTAATAGCAACAACGAAAACAATATTTACTTTATAACCTTTACAACTTCATGATGTCCAGATTGTAAAGTTATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGAAAAATTTTACATGTTCCAACATTTTTAATAATGAAAAATAACAAAATCATTCAAAGAGGAGAAAACTATTATCCAAAAGAAATTCTTTGTGATTGAGTAGAAAAAGTTTTAGAATAAAAAATGACTAAAGAAGAAATAATTACTTCATTAAAAAATGTTTCAACAGCACCTGGTATTTATCTCTGAAAAGATAAAAATGATCAAGTTATTTATGTTGGAAAAGCAAAAAACTTACGCAAAAGAATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGACAAGAAAAGTTCAACAAAATTAAATCCATTTTAAGTTTTAAAGATAACTCTTATATTAATGAAATTAAAGAAAAAATGTTCAAAGAAGCTGAGACTGAACATTTTGAATTAGCTCGTGATTTAAAACTTTCCTGAGAATATCTTAAAAAGCTTAATGAAGAACAAATTATTGAATTAAAAGATATTCACAATTTAGACGTATTTGCCTATAAAACAGTTGATAACATAATTTATATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTTAAAACATATGTTAATATCTTTAACGCTCCTTTAGAAGAATTAGAAAAAGTTGTTTCTAAAAAAATAGCTTTAGAAATCAAAAAACTTTATGAAAGTAAGAAATAAGCAAGTTTATTTTGCTTGTTTTTTTAATCTATTTTTTATTTACAATAAAATATAAATAAGAATAATAAGGAGATTTTATGAGTTTTAGTAAAAAGTTTTTATGAGGTACTGCTTTTGCAGCTAATCAAATTGAAGGAGCTTATAATGAAGAAAATAAGGGTTTAAATGTCCAAGATGTATTACCTAAAGGTGCTTTAGGTCATATTGATTTAAATTTAAAAGAATTTAATATTAAAAGAAAAGCTATTGATTTTTATCATAAATATTAAGAAGATTTAGATCTTTTAAGTGAAATGGGAATTAAAGTTTTAAGAATTTCAATTGCTTGAAGTCGAATTTTTCCAAAAGGTGATGAAAGTAAACCTAATCAAAAGGGTCTTGAGTTTTATCTTAAATTGTTCAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAATAATAAAAAAGTTGTTAAATACTTTTTAAATTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTATGCTAAAGTCAAAGAAATAATTAAAAATAATTACAATCAGATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGTGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGACAAAATCTTTATTTTACTGATGTTTTAGCTCGCGGAGTTTATCCTAAATATTTAGATAATTATTTTTTAAAAAATAATATAAAAATTAATAAGACTAAAGAAGAACTGGAAGTGATTAAAAATAATATAGTAGATTATATTGGTTTTAGTTACTGTTAATTCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTTTTTAAATTGTCCTTTATAATATAAATTGCTTAAAAAATAAGAAAGGACACTTAAATTATAATATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGAATTTCTAACAATTATTTTAGAAATAAAGTTCATATTATTTTCAAAAGTGAGGGTGGTAAGTTTTAAAAAATTATATATGTGATTATGTTAATAATTTAACAAGTTCATTCAAATTTTAGTTAATTTATTGAATTCTTATTTAGCTTCTCCATATAAGTCTACATTGAACTTAACATTGTTTTTTAAATCTGTTTCATAGTTTTTACATTTGAAATAAGTGAGAGGAAATAGAAACAATCCAATTAAATATATTAAGCAAACTAAAATAAAGTTAACAACAAGTTTCATAAAATAAACAAAGATAACTCATTTAACACTTCCTCAAGCTATTATGCTACTTCCTCTTCATCTAGGATAAAAACAATCCATATGATAATAAGTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGAAATACTCCTATTTGATAATTTGAATAAACAAATTATAAAATAAAGAAATAAAAACTTTATTTAAATATTTAAAAAGCTTAATAAAAATTAGATAAGTAATTATGTTAAATACTTTTTTTAAAATTCTTTAATTATGTCAGGATTTTCTAAATAACTTAATAAAGAAACATGAGTTATTCCATCTTGATCTATTCAGTTATTTTTTTCGCTATAATCAACAATAACTTTTCTAAAGTTGTTGTTAGTTAAAAAGAATGGTCTCAATTCTCTTTCTTTAACTTCTTGATTGTTTAAAGAAGTTGTTACTTGAAAATATACTTTTGTATTGAAATTTTGTGCTATAAAATCTATTTCATATTGATTATTAATTCATTGATTATTAATATTATTTTTTTGATTAATTGCCCCATAACTAATATCGAAGTAATTTATTTTTAGAGCATTAAAAAAGGCGTTTTCGATTAATTGACCTTGTCCTAGATTTCTAAAATTGAGAGCTACATTTCTTAATCCGTTGTCAACAAAATAGTATTTATATTGACCCGAAAGAAGTTTTAAACCTTTAATATCATATTTTCTTATTTTATCTATTAAGAAAGAATCTTCTAGTGCATCTAATATTTTATCTATTTGATAAGTAGAAGTCTTTTTATTATTTAAAGAATTAAAAGAGTTGGCAATTCTACTTGAATTACATTGAGATGAAACTATTGAAGCAAGATATTCAACTATGTTTAAAACATCTTGAGTACTATTAAGTTTATGTCTCTCAACAATGTCATTAACATAAACATTTGTTAAAAGATCTTTTAATATTTGTTTCTTTTCTTTTTCATTATCTACTAAAACTACTTCAGGCATACCACCATAAAGAGAATATAAATTTCAAGCTTCATTTATATCAAGATTACTAAAGTTTAAATATTCTTGAAATGATAAAGGCAAAACATTAATCTCCATACCTCGACCTGCAAAAGTAGTAGCTATATCTTTTGATAAGAATTTAGAATTACTGCCAGTAACATAAATATCAATATTATCTTGATCTTGAAATGAATTTAAAACCAATTCAAACTTTTTGACATTTTGAATCTCATCAATTAAAAGAAAATATTGTTTATCGTTAACAATTCTATCTTTAATGTATTTGTATAACTTTTGACGATCTCAAAGTTCTTCATTTTCAACTCGGTCAAGTTTTATTGTTATTATTTGGTCAGCTTCCACCTTATTAGCTAATAAATAACTATAGAATAAATTGAAGAGTAAAAAACTTTTGCCTACTCTTCTTATACCTGTAATTATTTTAATTTTTTTGTTATTGATTTTATTAATTAGCATATTTAAATAATTGTCTCTTTTAATAACTTTATTCATTTCTAACTCCTTATAAAAATTATACTATTTTCTTAAAAAGTTAGCAATATTGCCAATGTTTTAAAAAAATTACTTACTTGTTTATTAGTGATATTCTATTTCACATATAGGTGGAAATGCTAAGTGATAAATTTTTAAAGATTTGACATGTCCGCATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTCCAGTTTTAAATAAATACAAAAATTGTAGAAGAATAATAGTTAATGATAGTATGCACTCATAGTCCTATCAAATGATTCATAGATACAAGCGTCTATTTGTAATTCATCGCCAAAATGCATGTTTTCTTTTCTACACAATCTAACTCTTAGTCTCGCTTCGCTAAACAAACATAAAAAGTTTTGATAATTGTTTCTAACTTCTTCTATTTGAACTTTTAACAATTCAGCAATTTTAATCATTTTTCTTACTTTTCTTCTAGTTTTTTTATGACTAAAAATGGTTAAAAATCCTAAGCTTGTTAATCTGTTAACTAGAGTTTGATAACTAATTTGTTTTTCATAGCCTACATGAGAATAATCTACAATTCTTATTTCTTTTCAAAAAGATTTGATTGTTGCATCATTAACATTTTTGATTCCGTTAATTTTGTTTTTAATATATTCTGTGTATTCTTCCATAAAATCATTAACTGCATAATTTAAATTTTTCTTGATTGCATTTTTATTTAATCTATTTTTAGAAACAATTTGAATAGCATCGGTTTTTCTATTTAAAAAATCTCTAATATTGTTTTTGTATCTTTGAATAGATCTAATTGATAAATCAACTTTTTTAGCAATTTGTTAATTACTCAAATTCATTCAGGATGCAATTAATTGATAGTTTTTTTGTTTTTCCGATTTTGTTGGATTAGAACAAAAACTAAATACTAAATTGATAAATAGCTCATATTTTACAATTATAAATGCTAAATTGTTTTGTATCGAAGGTATGACAATCAATTTTAGAATTTATACTTATAAAATTAAAATGATATTTTTTACATATATTGAATTATGTTAATTTATATTTAGCATTTTTATATTGACCAACTTTTTTGATTTTATTTTGTTCTAATAAGACTTTAAGTTTTCTTTCTAATGTTCTTCTTGAAATTGAAACAAGTCGTTCACTTATTTCTTTAGTTGATAAAGGAATTAAGCTAGTAGTTAAAATTTCGATTATTTGATTTTCTAAATAATCTTTTTTATTTACATCTATTAATTTATTGTAGGAAATAATTAAGCAATCTAAAAAATAATCAATAAAAGGCAAATAATCGTTTTGGCTATACTGTCAATTAGTTGAGCTTTTTCTTAAAGTGAAATAATATTGATTTAAATTTTCTTCTATGATTTTTTCAAAACTAATGTATTTATTAATCATAAAATCATTTTGAAATAATAAACAGTTAGTTAATAATCTTGAAATTCTACCATTGCCATCACTAAAAGGGTGAATACATAAAAAATCCAAAATAAATGTTGGAATTAGTATTAAAGGATCAATTATTTTTTCATCATTTCTTTTATTGTATTCATCACATATTTTTGTTATGTAATCATCAACCAAAGAATGATCAACCGGTTCAAATATAACAGTTTTATAGTTGTTTTGGTCAATTGAAAAAATATAATTGTTCATTTCTTTAAACTTTCCTTTTAAATAAGTTTTATTACTATATTTATATAATTGATTATGTAAATTTAAGATGTTTGATTTAGTAATATCTATATTATCAAAATTATTTAAAATAATATTTAATACATCATTATAACCTTGAATTTCTAATTCGTTTTCGCCTGTATATTTTTTATTATTAAAAATAATATTTTCAATTTTGTTTGTAGTTGAAACAATATTTTCAATTTTGTTTGAAGAAGTAACACTTTGAAAAATACTATTTTTAATTAAAGTATTTTTAGTTTGTTCTGGAATATTAGAAAAATCATTTATAAGTCCATTTAACTCATAAATTTGTTTTAATTTTTTGTTAATTTCATTATTAATAATGTTCTTTAAAAATGAATAATCAAAAAACCTTTTTTCTTTTTTAACCATAATAATTAATTATAAACTAAAATATTTTTTACGACATTTTTTAAATTTTGTCGCTAAAGTTAATAATGTTGTCGTAGAAGTCTAAAATGACTTTGAAAAACATTTTCGAGGGGCATTTTTCAAAAAACGTGTTTCTAATTCTTGCCAAACAATAAATAATTTTGTAAATAAATGGTATTAAATACCAAAATGAAGCACTTTTTTATTAATGTTATGTAAAAAACAAACAAATTTGTGGTTTTATATTACGATATATTACATTTTAATTAAACAAATGATTTTATGTTTTTAAAAAAATCCAAATTTTATCAATAAAAAATGTCCTAAAAAAGTCACTTTTTCGAGGGACATTTTCGAGGGACATTTTCGAGGGACATTTTCGAGGGACATTCTTTTGTTAGGTATTATAAGGGCCTATTTATCAATAATTTCCCAATAACCATTTTTACCTTTTCCAACATATTTTATGTTTTTTATTTCTCTAATATATCTTGAAATAGTTTTTTCAGTTAAATTTAAAATTGCTGCAATTTCACTTCTTTTTATTGTAGAATTTTCTTTAACTAAATTGAAAATTCTTTCAATATTTAATTGTTTTTTGTTTGTAATATTTTTATTATCTGTATCTATATTAGAGGTCATTTTTCCAACTTTTAATGTAGCAATTTTTGGAATAGGAATAATCGTTTTAAATATGTTACTATCCAAAAATGTAGGTTTATTATTTGAATAAAGTTTTGTATATTTATAAGTTTTTCTAATTCCTGAACCTAATTCATCAGCTAAACCAATTTCTCTAAAAACTTTTGAAATTGGTGGATTTTTTGGATTTGGTCTAAAGGTTTTAGGATCTAATTCCTCTTTACTGCTTGAACTATTATCATTTTCAACAGTTATATTGTTTTCATCAATGACAATAGTAGCTGTCATATTGCTTGCATATTTTCTATGAGCTAGTGTATTAGAAATGATTTCTCTAAGTATCAAATTACGAGCACTAACACTTACAATACCATCTAAAACAAAAATATCGCTTAAGTGTTTTTCACCAAAATTAATTAATTTCTCATAGCTATCGATTAAATTGGTTGCTACTATTTCTCTATCATCATATCTATCTTTATTTTTAACTCTAAAAATAGCATCTGTACGATATTGAGGAAGAACAGACATAATAGTTTGATCTTTTCCAAAAAGCAAAATAGCTGCTATAGTTATTCCTTCTTTATTGGTTTCAGGATCTTTTAGAATTAAACCTGCGCTTTTAAGCATTTCCTCATCATTTAAGTTTTTTCACAAATGATTTTGATTTCTAATAATTGCCATTTTTCTTGCTTTATTAATAACTTTTTTGTCTAAATCATTAATACTTAAATTAGGATAAACTTTGTTTACAAAACAAATACTTTTTTTCTTTTGAAATAGTTTTCAAACTAATTCATCTTTATTAGTGATATCAACATCACCTTCAAAAGATCTTTCAAAGATTCTACCATTATGTCAACAAACATTTACTCCAACTGGAACATAAATATAAATTATTTTTTTATCATCAAAATCTATTACTTCAGGTTCAATAAATAAAGGAGGATTAATTTTAGTTGAATCATGAATTGAATTCAAGAAGTCATTTACTATTTTTTTAACTTTAGTTTTGTCTACACCTAAAACTTCACCATTGTCTCCAATACCTAAAATTAGATGACCTCCATTTTTATTGCTAAAAGCTGAAACGGTATCAAAAACACTTTTAGATAAGGCATCTTTGCATGTTTTGAATTCTATTTCTTCGCCTTCACCTTGGATTAAAAGTTTTTTCAATTCTTCTTTATTCATAATATCTCCTTATATTAAATATTTTATATTATTTATACAAATCTATCATCTAAAAGAAAATCATATAAACCTATTACTAAAATATTATCTTTAATAAAAGATTTTCCTCTAAAGTTCTTAACAATTAGTGATTGTAAAGTACCTTTTTCAACTTTTTTCTAATGGTCTTATTTTTCTTTCTAATACACCAGGATTATTAATGTCATAACAACATTGAATATAGACTTTAGTTATTGAATTTGTCACTACAAAATCAATTTCTAATTTTTCTATTTTTTGTCTTTAGTTCAAGTTTTCGATTACACCAGCATTAACATCGTAACTTTTTCTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAATTCATATTTTTATTTAGTTTCGTATTCTGCTAAATTAATATACCTTTCAAAAAAATGATCAAAAGTATAAAAAAATAACAGCGCCTAGAACACCACCAATCGTTCGACCTGTTGTATATATTATACCACAGTCGCTGTTATATGTTCAAAAAAAAGGAGAAATTATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGAGCTTATAAAACGCTTGTATGAAAATCAAAAGAACTTGGAACTAAAAAGTTTGTTAAAAGTAGAAAGATGAAAGTCATTGTACAAAATAACTTTTTAGGGCTAAAAGGTTCTACGGCTTACAGATACTTGAAAAATATATGTTTGGTTTTAGGTTTGAGAACTGATGAGTTATTTATTATTCAAAGAAGCGTCGATAACTTGCAGCATTTAAGCTTTAAGACTCAAAGGTATCTTGTAATACGCATATAAAGCAAATGAGGAGTAGTAATGAATAAGCTTCAATTATTATTACTGCGGTTTATTCATTACTGCTTGCTTTAGAAAATCTTATGGACGCACACTCGTGTGTTATCTTTCATATTTAACCTTTTTGGGGTTTAAAGACATTTTAGAATAAGTATGCTGATGATGTCCAAATTAGGTCGATAAATAATTAGGAGTATTAATGAATGTAATGAATTAATACGAAGGAGAAAGAAATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAGTTAGTTAAAGAGGAGGGAGAATGGATGTTTATATGAAGTTCAACTATGTATCTAAAATGACTAACTATAAAACTGGTTTAGGCTATTGTCATGATTTAAGTAGTTGAGTATCAAGTGGGACAGTTATTGATTATTTTGCAAGAGGAGATAAGTGTGTAGCATTTAGTCCTTTTGATGATAGCGAAAAAGATAGAATGCTTTTAGAGGAAGTAAGTAGAGAAGAAAAGATTAACTGATATAGAAATCATCTTAAGTCTAAAATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGAGCTGAGTTAGTTAAAGAGGAGTTTAGAAACTTCTATAGCATTAGCGGTTTTAATTTAGATAACCTTAGTTCTTATTATTCAATACATGGTAATACAGATAATCCACACATCCATTTTATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGAGATGCCAGAAAAGATGTTTTATCTTCGACAGAACGAACGCTTGAGAATGTTCAAAGCCAAGATTACAAAGACTTTATTGAGGCTTGCTGTTATGTAAGAGCTGAAATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGAAGATTGATTAATAGAGCTAAGAAGATTTTTGAAGCTCAAAGCATTCAAGAATACGAAAACATTATGACTTATTACAAAATATAGGATTTTATTATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGAAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAACAATATGCCATTAAGAAACGTAGCACTTGTATTGTTTTCAATATCAATTTTAGTTTTCTTCATATTTGCTAGCCTAGTGAATGTATTAAATATAAGTTTTGCTTGAAATATTTCTATTACGGTGTCGTCTTCATTATTAATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTCCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGATATAGAAATGAAATTGATAATAGCGGTGTAAGTAATGAAACTTTCGATAAGAAGTTTAAAGTTGCACCAAATTATGTGGGAAATCAAAACAACGCTAATTGGGTAATTAAGTTCGATTCAAATCTAAATAAATGATGAGTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGAGAGGACAACATGAATGAAGCTATAAGCGAAATTAATAATGTTGTTGAACAGCTAAAATGAGGGAATAATAGCGATGGTAATCCTATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTGAAATCCTAAACAAAAATGAATCTCAATTTGCTATATAAATGCAGAAACAAATAAAAGGTATTATTGAGCTGAATTATTTAAAAATGTTAAGACAATTGCAAATATTGTTCCTGAAACATTAACGGGTAATTTATCACATACTACGAGTGCTTTAAAAAACTTTACTGAAGATGAGTTTATAAAGAGATTAACAAGAGAAACAAAGAGTTTTGATTTACATGGTATTGTATCTTCGAATGAACCTTATGTCATCTTCATTCACTATCCAGACCATAAGCCAAGTAATCACTTCTTAGTAAGTATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAACTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGAAATTATCAAGACTTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGACAAAAGATATTAAGTTTCCAACAAAAATACAAAAACAAATTAAGTAGTGAACTAGTGAACGATTTCACGAAGTCGATAAATATATGTTTAGACCAGATGAATGATATTAAAACAGCTGGAGCTAAAACATAGAAGAAGGCGAATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAATATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCTAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAGGAGGAACTATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAGAAAGGAAAAAGAATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAAAGGAGGCAGATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGAGTGAAAAAACGAATAGAATACTGAAAACAAAGTAAGAAGAATAGTGATTTGCATTAAAATTATTATGTAAAGGTATTATTATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGAAAAAAGAACGAGTATATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGAGTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGAAATGATTTAAGTAGTTTTGCTTGGATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGAGAAGTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGAATTGCAATTTGGAATAATAGAGTTAATGGAAGCTTGGATACAAGCACTTTAGATTAAAAATTATGCTAACTTTACTACATAATAATGTGTGCAAAGAACTATTTTTCTAATAGATGTTTTTCTATTAGTTTTTTTGTATTAATTTATTAAGACAAAAAGGAGAAATTAAAAATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGAGCTGGAATTGTATTAATTCTAGTTATAGTGGGTTGAGGAATATTTGAACTAGTTATTAGTATTCAAGCAAGCGGGGGAAAGACTAGCTAATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGACTGGTGATGTTTTTTGTGCTGATTATAAAGTTCAGTTTTAGTGAAAGCGAAAAGAGTGTATCACTAATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGAGCATCCATTTTAATAACTGCAGCGTTTCTATCAATTATTGGAGCGGTGTTTATTGCTATTCCACTTGTTTTAGGGGGGATGGATGCCATAGGAAAGATATTTCCGTTATTCTTCTTGTATTTAATATTTCCAATTATTTTACCTTTGAGCTTGCTTGATGATGGCAAGAAAGTAAGGATATGAAAGGATAAGTATGTGGGAAATATGTTATCGGTGGGGGTATTTTTCCTAGGACTACAAATACTTTTTGCTTTCTTCGGTGCTGTAAATCTATTTTTAACGCAAATATTCATTGCATCATCAACAAATGGTTTTGGATGAGTATTACAAGCTTTAGCTTCAATAATTATTTATGGTGCTACAGCGTTAAAATACAGCGAAATAAGCGAAATTATAACTTCATTTATTGGAACTAGTGTAAGTAGTAAAAACACTATTAAATCGGCTTCAGCTGCGGGAAATAGTGCTGCAAAAACGGGTAGAGCTGCTCAAGGTGGAGCACAGCTTGCTGAAAATGTAAAGAAGATGGGCTGAAAAGGATTATTCCTTAAACCTAAAGAATAATGAGAAGGGAGAAATGTGCTACAACCTAAACGATTAAAATATAAGAAGGCAAAAGCCACAAGAAGATTTAGTTGAAAAGATATTATCGAGTTTTTTGTAATAACTTGTATATCTTGATTTATTTCATTTGTTGCTTTGTATCCAGTTAAGCAGACTTATAAATGAATTGTTTTTAGTGTACTTTTGGTTTTATTGTTTATTTTGATATTACCAACACATAAGAATAATTGCAAGATATATGAATTATTAATTAGAATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGAGCTGAAAATGAAAATACTAAAGTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGATGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGAAATAGTTATTTTAAAGCTAATGAACTTGATTTTAAGAATGAAGTTAAAAGCAATTTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGATTAAAGAAGTTATTCGATGTATCGGGAACAGTTGTGTGAAACAATTTTCCATATAACGATCCAAATGTTGTAAATAAGATAATCGATAAAGCGAATAAGAAAAATATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGAAATATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGACCAACTATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAGTCAAGAAAATAAAAAAGGTGAATTATATGTAAAAAGCTCTAGAGCCTCAAAAAACGGCTCTAAATGTATTTCTACGCGTCTTAGTGAACCAGATGATGTGGCGTTATACGAAAGATGGGTAAAAAGAATTAAACAAAGTGGCTCTACTCCGTATGTAGAGCTTAAAGATATAATTCGCAACGCTTTAATTAAACAAGAGAAAGAGGAAATAGCTAAAGATTTCAAAAATGATTTATTTTATGCATTAAGAAAAGTATCTTGAGCGTCTTTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAAAATAGTCTTTAAACATGCACACGTGCGCACACGGGCATAACTAATATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGATACGATGATAAATATTATCTAAATTATTTGCATCCTCGTGAAAAAGAGAATTGAGTAATTTTTGCTTCTAATTTTATAGAACAATTAATAAAATGAGACAAAAACAACTTTATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGATTCATAGAGGCTTTAAAAGAGTGTGTTTCAAGTGATAATTGTTTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTCACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGAACAAAAGAAAACATTAAAGCTGAAGCTGTTGGGAAGTGGAATCAATTTTTAAGGGTTTTAAAAGATATCGTTTTAGGTAATTTTAAAGTTTTTGATTCATTAAAGGTAAATATTTTAGAATCAGATTTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGATTTGGTGGAAGGTATGTAGTTAGAGGGCCAATTAATATTTCTTTTAAAGCTTCAGAGGATGAAACTGAAGTTTTAATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGAGAGCCGGAAAAGAATATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGAATTAAAAATAAGTTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGAATAACTACACTATTTCAAAATTACTTAAATGAAGCTTGTGGTATCCAAAATGATGACTTGGGTAAATTAGGATATGACAAGATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGAGGTAAATTAGAAAATCTTAATGGCTTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGAAATACGCCAGAAAGCGAAAAGCGTTGATTGGATAGGGCTTTAAAAGTTTATATTAAAGATATTAATGTACCAGATAATGACGGTAAGGGATGAGCTTTTACTTTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGAGAAATTGAAGGACATAAAGTTCCTATTCCGTATCAAATAAATGATACTGAGCTATTTAAGAAGTACCACGAAGCTTTAGAAAAGGCAATTAAAGAAATACAAAACGATAACTTTAATAAAAGCTTAGATTATAAAGCTAGAGGCAAGTATTTTGTTTTATTACCAGTTAAAGAAGGCAATAATATTAATTTTGCCTATGCTATAGTGGTGAATAATATTGAGGCAAATTATAATGCTTTTGATGATAAATTATGATTTAAAGAAATACAAAACGATAAGCATAAAAAGCCAAATGGTGAAGATTTAGATGTTGATAAAACAAAAGCAAGCCATAAAAGTAATTATTGAATTTGAATTGTATCGGTTATGCTTGCCTTAGGTTTTGCTGTTGGTGGCATATATGGTATTAGATGATACTTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTTCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAAAAAAATGTTAGGCACTATATTTTTAACTAAAACATTATTAAGAAAATATGGGTGAAGTTAAGAATTTTATAAAATATTGTTTTTTTACTATATAATGTAGACATATGAACCTAGAGAGATACACGTAAGTAATCGAACAGTAGCAAGCTTAGCGTGTAGCTACAATTACTCTAGGCTGGGTCGTCTTAAAAAGAGGTTTTATACCTCTTTTTTTGTATAATTTTATTATGTCTTTAGATTTATGAAAACCTTTAAAAATTAATAACCCATTATTAGAAAATATTTTCAAACTACCTATTGATGGCAAAGTTTTAAATAACGGAAAAATTCAAGCCCACCCTTTTATAATTTTTTAGTAAAAGATTATCAATTTGTTTAAGAGTAAACAATGCAAATAAGATTTGAAAAATTATTAATTTTTTTATTTATTTGTAGTATAATTTAAACAATAGTCTAAGAAGGATTTTGTTATATGAGTAATCGAACAAGCAAGCAGCTCATATTTTATCTTTAAGCGCTTGATGATACGCCTTAGACTCTAGTTCGTCTTAAAAAGAGGTTTAATCCTCTTTTTTTGTATAATTTTTATATGAGTTTAGAACTATGAAGACCATTAAAAATTACAAATCCTTTATTAGAAGATATTTTTCATCTAAATATCGATACAAAATTTAAAATTCATCCTTTGATAATTTTTGAAAATACAAAAAATAAAACTTATTATTGTATAAGACTTCAAACGGCAAGAAAAAGTTCTAGCAAATATAATTTAATAATTGATAACAGCTCTTATCAAACCAACGAATATTGAAAGAATCATGAAAGTGTAGTAGTTACTAAGGATATATTTATTATTGATAAGGAATTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGAAAAGACTTAGATAAGACGAAGGATAACGTTAATATATATTCTTTTGAAAATATAGGTTTTTTATCTAAAAGTAGCAAAAGTGAATCAAGCTATATTGATCCATACGATAATAAAAATATCGAAAAAGCTAAGGATAAAGTAGCTAAGGAAATACCAATAAAAGAAAGCAAAACAGACAAAGACAAAACGAAGCTGAAGTAAAACATATAAAATTATTAACGGCGTGAAAACGTCGTTTTTAATTGATTATGGAGGGCGCTGTGAAGAACAAAATGAGTAAAACTTTAGCTGACAATTGAAATGCTCAAGCAGAAGTTTTTAAGAATAGCGAAGAGCGTAAAATTAAATATAATGTTGAAAGAAAAATAGAAAGAGATATTTTTATTTGGGGCGAGACAGTAAGAATTATTTACTACATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGAATAACAAAGCAAGAGAAAAATAAAACTGTATATGCTAATGCTAAAAAGTTCAAGAAATATTATAAAGAAAATAGAGAATATATTTGAAACTCAGTGCTAGTTAAAAACTACTTTGGAGGCTGTGCTGAAACGGTAGTTGGGCACGATTTAAAGAGGTTTTGCTCAAAGAAATTTGCTACATTTTCACTAAAAACAATACAGTTAAGCATTGCAAAAAATATGGAAACAAAAGCAAACTTATTTTTCATTTAAGCAGTATTTTTAACAAAGTGCATGCGCAAAAGCAGTATTAATATATTTTTAAAATAAAAATATACAAGTTAAAGTGCATGTAAAAGTGCATGCGAATTTAGGCAAAAACGAAAAAAGTGCATGCGCCTAGAAAATATGAAAATATTAATTTTTGATTTATTCTATGAATAAATCATTTCAAGATAAAACACGCTATGGTAAATAGCTTTGTAGTGTCTTTATCTTGTACTATTCAAGTAGCGCGTGTGCGCGCAAAAGGAAACTGCATGCTCTTCACATGCAGTATTTTCCTTTGCTACAAAATGGATTATGATAAAATAATTACGAAATAAGAAAACGTAGAGAATCTTATAACACACCTTTTTATATTAGTTCCCTTTTTCTTAATAATTCAAAGTAAACAATGTTTTCTATGTTCGACCAAAGTCATCGAATTTAAAATCATTTAAAGCATTCTTTAAACCATTAATATCATATTGAGTAACTTTTTTAAAAATAAATGATTCTTAATAATAGTTACAATAAGTATCTATTGTTCTACTATCAATTTTAAAATTGTTATTACTATTTATAAATTACTTATTTTTTAGTATTAGTTAAAGATGAAGAACTAGAAGCTAAGAATTTTAATAATTCCTCTATTTTATAGTCATTTTTGACATTATAGTGTTCTAATATATCACTTAATAAGAATGTTATTAAATATTCTTTTAAATATTTTTGTTTGTCATTGATGTTTTCTTTTAAAATAACTAATAACATACCACCTTATTTATAATAATCATCAAAATATAAATCATATTCATTTTCTTTTAAATTGAAATATTGAACTATTTCTTTAAATGAATATGGACAGATTTTTATTTCTTCACCGCGACCTATAAATTCTATAGCTATATCATTTGAAAGAAATTTTAAGTTACTACCAGTAACATAAATGTCAACTTGACTTTTGACTAATCTAAAAGTGAATTTAATAATAACGGAAAGTCTTTAATGTTTTGAATTTCATCAAGTAATAAATAATATATTCCTTGATCTTTAATTAATTTTTTAATATGATTATATGCCTTAGTGGCATCTAATAATTCTTTGGTTTCAAGGTTATCAAATAATAAAGAAATGATATGATTAATATCTACGTTATTTTGAATTAAGTAATTATAAAAAAATTATTTAATAAAACACTTTTGCCACTTCTTCTAATACTTCTAATACCAGTAATAACTTTAATTAATCCATTGTTTTTATATTTGATTAATTAATCCAAATAAAAATAATGTAATTTTGTGAAATTTAACCACTCCATTACATTATAGTTCAATTTATTTTTAATCTATAAATCCTAAATCACTAACTTTAAATTTTGCATACTTTTTATTTAATTTATAGGAAGCAATTCTTTTAGTACCACTATTTGGAACTAAAATATCTTTGTCTATTCAGTCTTTAGTTCATTTTGATATAGCTCTAGAAGTAACGCCAAAGACTAAAGCTAAATCTTTAATAAGAATAATACTTTTTTTATTTTCTATGCAATATCTTAATAAAGTTAAGTCTTTTTTATTAAGATTATTTAACAAATCATTATAACTATTATCTTTAGTGCTTGCTTCTAAAGCACTTAAATATATATTTTGTGCATTAAGAGCCATAATTTTACAAAAGTAAGTTATTCATTTTTCTAAATGTGGCAGTTTATTACGTCCATTATAAAATAAAGGACTTAAATCCATTTGTAATGCTTGATAATATTCATTTAAGTTATTACTATAATATTCTTCAAAAGAATTAAATCCTTTGAAATCATAATCATCTAACATTAATGAATAAGTAGCTAGTGCTCTAGCACTACGACCATTACCATCTTCAAAAGGGTGAATTGTAACAAATGCATAATGAATGATAGCTGCTCTTATTGCACTTGGTAAATGTTGATTTTCATTTATTCAAGTTATTAATTCATCTATTAGAATATTTATATCCTTAGCTTCTGGTGGTATATATTCAGGACTTTTAGTAACTGAATCATATACAGCAAAAAGTACTCCAAGAGGAGTAGGTTGTCGAAAATCTATTTTGGTAATTCTAGAACTTTTAGCTTCAATAATATTATGTAAACTAAAAATAAAATCTTTAGTTATTTTTATTTTAGCTTTTTTACTTCTTTCTAAGAAACTTAAAGCTTCTCAATAATTTAATACTTCTTGTTCAGCTTTAATTCTAGTTTGATGTGTTTTATTGTTTATAACGTTTTTAACTTGTTCAATGTTTAAAACATTGCCTTCAATACTTGTTGAAAAATGAGTTTGTTTAATTTTGGCTTCAAATTGAATTCTTTGTTTACTTCTTGTAGGTAAAGACAAAAATTCTAATGAACTTTTGTATTTTTCAATTTTTAGAAGATAATTGACAAGCTCATCAGTATATTTAAATGATAGTTCTAATTCTTTTTTCATGTAAATATTATACCAAATAAGGAACAAATAGAGAACAAACAAGGAGCCGTTTCGTTCTTAATTTATTAAATAAAAGTACAAAAAATCTTCCCTAAATAATGAGAAGATCTTTTCTATTATTGTTTATCTCTAACAAATGTTATGACAAGACATACTTTTCAATTCATTCAATAATTTACTGCTATAACTTCTCAACCTTTACTTGCCATTTCATTCATTAAATCTTCAGCTTTTGATGGGTGTTCTGTTAAAATTTTATATTCTTTCATATAGACTCTCCTTTATTAATATTTTAGTATTGTTTTAACAACTAATAATATTATATATTAGAGCGAACCTTTTTCCAAAAAGTCCGGAATTTGTGGTTCACTATAAGGTTATTAGAACTAATAAAAGAAAAGAATTTTTTGTTTCTGAAAACACCACAACCCCTTTAACTAATACTTTAATGGAAAAAAATAAAAGTAGAATCTTCAAGTTCTCCTACTTTTAAAGTACAAGTTGAAAGATCTTTCAAAACAGCTCAAGCTAATTATTTACCAATACTAAATGGTAAAAATTTAGAAGAATCTAATAAAATTGCAAATGACCTTAACAACTACTTTAATGAAAAACTTAGAAAAAATATTGAAGTAAAAGAAAATCATTTTGGAAAAGTAATAAATGGTGATTATAAAATCATTGAGAGATTTATAAGAATAGTAACCGCTAACACAATACAATATGATTACAAATATTATTATCCTTACAATCCAGAAAATGGTAAAAGAGTTTTTCTAAATGATAGATCAAAAGTATATGTGCTTATTGATAAGGATGGTAGTATTTATATCGACATTTTAGGAATTGAAAAATTTCCTACTAAAACATTGACTAGAGAAGAACTTAACGAATATCAACTTTTTGTACACAATTTAGGTGTGACAGAAAGCACAATATCAAAACTTCTTTATACAAAAATCAAATCAACAAATAGTTTTTATAACTTAATAGAATCTAAAATTGAAAAATTAAAAAATCTTTTACTCACTCTAAATTTAGACAAAAATAAATTAGAAAGCGAAATAAAAGATTTTACTCATAACATCTGTAAGATCAATAAAAAAATAGGTGAATTAATTCAATTCTAATTTCAATTATTTCTTTCAGATGACAAAAATATACTCTTTTTAAAAAGAAAGAGTTAGTTTTTTGTACTAAAAAATTAAATTTTTAATTTTAAACACTGTTTTATTTTTTTAACTTTTCCTATAAAAAAACATGTTTTTTTGAAAAAAATTGACAAAAATTTAATAATTTTGTATCATTAAATTAATGTCAAAAAAATATGAAAAAAAGTATGACAATCCGTTTTAAATTTCACATTTTGCTTTGTTAAATTTTATAAAAATTCATATATGCTATTATGTTAAAAAATAAGCAATATTGCCAATGCTTTGACAAAAATTGCTTATTTGATTTGATTATTAATCTTCTCAAATTAATTTGCCTTGAATTCTTTCTCTTTCTTTATCTATTTCAAGGATTTGGATTTTAATGTTATCTCCTGTTTTAACTACTTCGCTAGAGTGTTTGATGTAGTGATCTTTATTTCTTTTCATATTTGAAATGTGAATTAAAGCAGCTTGCTTAATTCCAATATAAACAAAGATACCAAAGTCTGTAATATTTTGCACTGAACCATCTAGAATCATACCTGGTTTTAAGTCTTCTAACTTAAGAACATCTTTTTTAAGAATGTAGCCATCTTTGTCATCACGAATATCTTTAGTTGGATTAGATAAAGAATCTAATATTAACTTAACATCATATTCATTTGAATTAAAGTCTTTAGCTAATTTATTAGCATCTTGTTGTTCAATAGTTTTTAAGTCTATATCATTTAGATCTAATTGAAGCGCATTAACAATATTAATTGCTAAAGGATATGATTCAGGGTGAATAGCTGTGCGGTCATAAAAGTTTTTAGAATCATGGATTCTTAAGAAGCCAACCGCTTGTTCATAGGCTTTTTCGCCTAAACCTTTAACCTTTAATAGTTCTTTTCTTTCTTTAAATTTATTGATTTTTTCACGATAATCAATAATGTTTTGAGCTAATGTTTTAGTTAAACCTGAAATGTAGCATAAGATTTCTTTAGTAGCACTATTTAAGTCAACTCCAACCATGTTAACTACTTTATCAACTTTAAAGTCTAAAGCCTTAGATAAATCTTTTTGATTAACATCATGTTGATATTGACCTACCCCTATAGCTTTAGGATCAATTTTAACTAATTCATTAAGAGGATCTTGGAAACGACGTCCAATATTAACAGCTGATCTTTCTTCAACACTAAAATCTGGAAATTCATCAATAGCAACTTTAGAAGCTGAATAAACACTAGCTCCTACTTCACTAACAACAGCATAAGAAATATCTTCATTAGGATTTACTTTTGATCTTTCTTTTAATAAATTAGCAATAAATTCTTCAGTTTCTCTTGAAGCTGTTCCATTGCCAATAACAATAATATTAACTTTGTATGAATCTAATAGTTTATTAACTATTTCTTTAGCTTTATCAACATAATTTTGAGGAGGGTTAGGATAAATTAAAGCTTTTTTTAAGAACTTACCTTGAGGATCTAAGATTGCAATTTTGCAACCATTAACAAAAGCAGGGTCTATTGCCATAACTGTTTTATTTTTAACAGCAGGTCATAGTAACATTGTTTCAAGATTAGTTGCGAAAAGGTTAATTGCTTCTTTTTCTGCACGAGCAAAAAGATCACTTTTGATTTCTCTAATAATTGAAGGTAAAATCAAACGATCTAAACTGTCTTTTAATGATTCGTTCAAAATCTTGCCTGTTCTTTTGATTTTAAAATATTTGTTGTTTAAGTCATAAGTAATTTTAGCTAAATTATAAGTCATGTCATAACTTAAAATCTTTTTGTCTTCACCACGACTAATTGCTAAAACACGATGGTTAGGAATAAACTTGATTCTTTCTTTATAATCATAGTATTGTTGAAATACTTCTTTTTCATCTTCAGCATTCTTTTTAACTTTTGTTACAATGAAACCAAAATCATAAATTTGTTTTTTAACATATTCTCTATTTTCAACATCTTGAGACATAATTTGGCTAATAATGTATTGAGCTTGCTCAATAGCAAATTCAACTGTTGGTACTTTGTCATTTAAGTATTTTTTAGCTTCTTTATAAGGATCAAAACTTTCATCAGTTGCTTCCATAATTGCTTTAGCTAAAGGTTCTAAACCTAAAGCAATAGCTTCACTAGCTTTAGTTTTTTTACCAACTTTAAATGGTTCATAAATGTTTTCAACTTCAGCTTTAGTTTCAGCTGCTTTTAATTTATTTTCAATTTCAGGTGTGAGTAATCCTTTTTCTTTTAAGATGTCAATAATAGCTTCTTTTCTTTTGTTTAATTCAACATTGTAAACATACATGTCACTTATTTGTTGAATAACTTCTTCATCAAGTCCACCAGTTACATTTTTACGATAACGGGCAATAAAAGGTACTGTTGCTCCTTCATCCATTAACTTAAGAACAACTTCAACTTGGTCTTCTTTTAAGTTTAATTTTTGTGCTACAAATTTTTTAGATAAATCCATATTTTCCTCCAATTAATTAGTATGTTAATTATACATATAATTTGCATAAAAGTAATTTAAAAATGTAATCATTTTTGACTAAAAAACCTGTTTTAATAATTGATTAGAAAATAATTTGTAAATAATGATTTCAAATCACAATTTTTTTCTTTTTTTTAACTAAAACAACTATAAAACAGTTATTTAGGAAAAATCAGTGAAGAACAAAAATAAACAAAAAAATTATTTTATTATTAATATAAATATTTAATATTATAATTAAGTCTATAACTATTTAAATCCTATTGTATTAAGGAGAATTATCGTGACTAAATTTATTGTGACAACTGGTGGAGTTTTATCTGGTTTAGGTAAGGGTGTTACCAGTGCAAGTATTGGAAATTTATTAAAGGCACAAGGCTTTAAAGTCTTTGCTTTAAAACTTGATCCATATTTAAATATTGACCCAGGAGTAATGAGTCCCATTGAACATGGTGAAGTATTTGTAACTGCTGATGGTGGTGAGACTGACTTAGACTTAGGACACTATGAAAGATTTATTGATGTTAAGCTAAGCAAGGAAAGTAACTACACAAGTGGAAGAATTTTTACTCGCATTTTTGAAAAAGAACGTCAAGGACTTTATGGTGGAAAAACTGTTCAAATAGTACCTCATGTTATTGATGAAATTATTAGCATTATCGAAAATACTGCTAAAAAACAAAAACCAGATTTCATGTTAATTGAAATCGGAGGAACTGTTGGAGACATTGAAAGTAATCCTTATATTTATGCAATTAGTAAGTTTGCTAGTTTAAATCCTAAAAGTGTTTTCTTTGCTCACTTAGCATTTATTCCTTATTTAACTGCTTCAAAAGAATACAAATCAAAACCATCTCAAGTTTCAATTGCTTCTTTAAGATCATTTGGAATTAACCCTAATATTTTACTTTTAAGAAGTCAAGGCGAAGTTGATGAAAGAATTATTAATAAAGTTGCTGAAGCTTCATTTTTGGACCCTAATCATGTAATTGGAGTTCCTGATAAAAGCAGTATCTATGAAATTCCAATGTTCTTATATGAACAAAATATTTTAAAAATAATTTACTCACATTTTGGAATTAAAAAACAAATTAATGACAAGGTTTATGATTCATGAAAAGTATTTTTGAATAAATATCACGCAACTAAAAATTATAAAATTAACTTACTTTTAGTTGGTAAATATACTGAATTAGAAGATGCTTATTTATCAATTATTTCTTCATTAAAAATAGCTGCTGTTCATCAAAATGTCGAATTAAAATATGACTTAATTGATGCTGATTTAGTTAACAATAAAAACATTGAAAAATTAACAAAAGCACATGATGCAGTAATGATTTTACCAGGCTTTGGTGCTAGAGGTTTTGAATCTAAAGTTAATGTTGCGACTTACACAAGAGAACACAAAATTCCTACTTTAGGTATTTGTTTAGGTTTTCAAGCAATGTCAGTAGCTCAAGCTCGATTATTAGGCATTAAAGATGCAACCAGCAAAGAATTCGCTGAAAATGGTAAAAAACAAACTTTTGTTTTAACTCCTTTTTATGAAAATGGAGACAAAATGGCTATTGGTGGCACACTAAGACTTGGTGAAGATCAAGTTCAAGCTAAAAATAACACCTTAGCTCAAAAAATTTATGGTTCAAATATTTTCTATGAACGTCACAGACATAGATATGAAATTAATCCTGAATACTTAGAAAAATTACAAGATGATGAATTTACATTCAGTGCAATTCATCCAACTAAAAAAGTGGCTGAAATTTGTGAAGTTAAAAACCATCCTTTCTATTTAGGTGTTCAATATCACCCAGAATTTAGTACTAGAGTTTTAGCTTCAAATCCACTTTTTGATAATTTCTTCCAAGCAATTATTAAAAATCAAAAATAGGAGGTCAATATATGGCAATTGATTACAAAAAAACATTAAATATGCCTCAAACTAATTTCGATATGCGTGCTAACTTAACAACTAAAGAGCCAGCATTCCGAAAAGAATGAGAAGACAATAAAATTTATGAAAAAGTATTAAATAAAAACAAAAAGAATACACCTTTCATTTTGCATGATGGTCCACCATATGCTAATGGTAATTTGCATATCGGACATGCTTTAAATAAAATCCTTAAAGATATTGTAATCCGTTATAAATCTTTAAGAGGCTTTTATGCTCCATATGTTCCAGGTTGAGATACGCATGGTCTTCCTATTGAACACAAAATGCTTGAGGAAGCACACTTAAAGAAAAATGAATTAAGTGTTGTTGACTTACGTAAAAAAGCTGCTCTTTATGCTCAAGATCAAGTTAATAAACAAGCTGAACAATTCAAAAGCATGCAACTTTTATGTGACTTTAAAAAATACTACATTACTAAAGATCCTAAATTCGAAGCTCAACAATTAAGACTTTTTAAAAAGATGATTTTTAGTGGTCTTATTTACAAAGGATTAAAACCTGTTTATTGATCACCTTCAAGTCAATCAGCATTAGCTGAAGCTGAAGTTATTTATAAAGATATTAAATCACCTTCAATTTTTGTGGCATTTGAAATTAAAGATAATTTAGGTAGCCAAAAAATTAATGTTAAAGATAACTTAGTAATTTGAACTACTACACCTTGAACTTTACTTGCTAATGCTGCTGTAGCAGTTGGCCAAGATATTCAATACTCAAGAGTAGAACATGATAAAAAAGGCTATATTTTTGCTTCTGATTTAGTTGAAAAAGTAGTTAGTCAATTAGGCTGAAAAAACTACAAAATCACTAACACTTTTAATAGCCAAGAAATCTTAAATATGCAATATGTAAGCCCACTTAATAAAAATGTATGTCCTGTAGTTATAGGCCACCACGTTACTTTAGAAAGTGGTAGTGGTTTAGTTCATATTGCTCCTCTTTTTGGTGAAGATGACTTCCAAATTGGAATCAAAAACTCACTTAAAATGATTATGCATATTGAAGATGATGGAAAAATTAATAAAGAAGGTGGAGCTTACCAAGGTCAATTTTATGAAAAAGCTAATGCTTTAATTATTGAAGAATTAACTAAATCTAAAAACTTACTTTTAGAAAGCTCAATAACTCACTCATATCCACATGACTGACGTACTCATAAACCAATTTTATTCAGAGGAACACCTCAATGATTTGTTTCAATAAATAAAATTAAAGATCAAATTTTAAGCCAACTTGAAAAAGTTAAAACTTATCCAGATTGAGCTAAAAAACGTTTAAGCAACATGATAGTTGATAGAGATGACTGAACTATTAGTCGTCAAAGAACTTGAGGTGTGCCTCTTATCATTTTCTATGATAAAAGTAAAAAACCAGTTTTAAACGAAGACATTTTTGATCACATTATTAAGCTTGTTGAAAAACACGGAACTGATATTTGATATCAAAAAACTGCTGATGAATTATTACCTAAAAAATACCAAAACAAAGGTTATACAAAAGAAAACGACATCATGGATGTTTGATTCGACTCAGGTTCAACATTTATGGCAGTTAATATTGATAAAGGTTTAGAAACTCCTTATGATTTATATCTTGAAGGTAGTGACCAATTTAGAGGCTGATTCAACTCTTCTTTAATTAACTCAGTAGCTTATAATGGTAAAGCACCATACAAAAACATTGTTTCACACGGTTTTGTTTTAGATGCTAAAGGTGAAAAAATGTCTAAATCAAAAGGTAATGTTGTTGATCCTCTTAAAATAATTAGCAAAAATGGAGCTGATATTTTACGTCTTTGAGTAGCCAATTCTGAATACTCAAATGACGTAAGTATTAGTGATAATATCATTAATCAAAACAGCGAAATTTACCGTAAAATTAGAAACACAATTAAATTTATGCTCGGTAATTTAAATGGTTTTAAATATGACTCAAATCTCAAAAGAATTGGCGTACATGCCTATATTAAAGAGCAATTGGAAAAAGTTAAAGCTCAAGTTATTGAAGCTTATGATGAATATAAATTCATTAACGTTATTAAACTTTTAAATAATTATGTAGTTGAATTATCAAGCTTTTATTTAAACATTTCAAAAGATGTTTTATATGTCGAAGCTAAAGATTCAATTAACCGTCTTATGACTTTAACAAATATTTATGAAATTGTTGATTTTCTAATTATTGCTCTTGCTCCAATTCTTCCAACTACAGCTGAAGATGCTTACAAAAACTTTAATAAAGTAAATAAAGCTGAAAGTGTTCATCTCGAAACTTTAGCTAACCCACTCAAAGTTAACGAACAAGTTTTAACTCAATGGCAAGACTTTTTTAAACTTCGTGATGAAGTAAACTTATCACTTGAAAATGCAATTAAAGAAGGTTTAATTAAAAGAACTAATGAAGCTAAAGTTGTAATTAAAAGCGATAGCGACTTTATTAAAAGTCTTGATTTAAGACAATTATTAATGGTTGGTAAAGTTGAATTTGGAGCTCAAACTTCTGTTTCAGTTTTCAAATCAATTAAATGCCAAAGATGTTGAAATCATTTCATGCCTTCACAAATTAAAGAGGAACTTTGTCCTCTTTGTCACAAGGTAGTTTTTAATGAGTAAAGAATACAATGGCTATGAGCCAGAAAAAGAACCATTAAAAAATACAATTAGTAAAAAATGAAATGATTTTATTAACTACTGCAAAAATAACAAAAAGAAAATCTTAATTGCTTTTGGAATATTTTTTGCTGTTGCTTTTGTTGCCATTCTAATTGATCAATTAACTAAAACCTTCTTATTCGAATGAAATGAAGAACATAATGGTGGCAAAATAGGAAAAGTTTATTATGAAGGAACTTTACTCGGAATTAGATCTGTAGGTCACTATGGAGTAACCTTCATTCCAAGTGTTGAAAATAAAAAAGGTGCTTTAATCTTTATTCAATTTTTAAGTATCCTAATTTTACTTAGTTTATTATTAGTACCACTTTTATCAAGACACTTAACCACTATTATTATTTGTGCAATTATTTGAGCCGGTGACTTTGGAAACATGTTAGACCGCTTTATGTTTAATATGATGGTTAAAGATATTTTCTATGTTCCTTTTATGGAAAAGTGAACTGGCAAAGTATTAGGAACCTTTAACTTCGCAGACTTATGTATTGTTTTAGGCTGCATCTCATTAGTACTCTTCTTTGTGATTGAAATGATATTAGATAAAAGAAGAGAAGATACACACCAAGAAGCACAAGAAATAATAGATGCAATTCAAGAAGACAATAGTCAAAATAATACTGTTGAAAATGATCCAAAAGAAGAACAAGAAGACAATAAAAAAGAATAATATATAGGCTTTAACATGATGTTAAGGTCTATTTTTTATGGCGACATTTATACCGACAAATAAACCGACATTTTACTTGGCGGTTTAAATTAAAAAGTATATAATTTTCATAAAGAGGTAAAAAATGAAATTTGTTGAAACAAATAAAATCGAATTAAAGGAAAAATTAAATGATAACTTAATTAGAGAAATTGTTGGCTTTTTAAACACAGAAGGCGGAACAATTTATATAGGTGTAAAAGATAATGGTGAAGTAATTGGCATAAAAGATGTAAATAAAGCTTTTACTGAATTAAACGAAATTATTTGTGATCAAATTGAACCAAGAGCGGAAAAAGAAGTAAAAAGTGAAATAAAATTTGAAAATAACAAAATGATTATGGGAATAAACGTTCAAAAAGGAGAAAAAGCTATTTATTACATAAAAAAATATGGTTGTTCTTCAAAAGGATGTTTAGTAAGAGTTGGGACAAGCACAAGAGAAATGTCAAATGAAGAAATAGAAGAAAGAAACAAAATTAGATTTTATGATGAGGACAACATAATAAATTATGTTAGTGATTTGGATGATTTGAAATTTTCAATATTAAAAATAAATTATGAACTAAATAATTTACATTTAGATGATAAAACATTTGAAAAAAATTTACATTTAAGAAATAAATTAGGTAGTTACAATAAGCTAGCTGAATTGCTAGCTAATGAAAATAAAATTGACTTGAAATATGTAAAATTTCAAGGAAATGATAAAACAGTCTTTTCAGAAATACAAAAATTCAGTAATAAAAGTATATTGGTTGGTTATTCACAAATTTTAGCAAGATTAGAAGTTGAAAATAAATGCTATGTAGATACTAGTTCAATGCAAAGAATTGAAAAGACTTTATTTGATTTTAATGTAGCTAAAGAGGCTTTATTAAATGCAATTGTACATAATGATTGATTGAGTGGCGTACCAACTATCCAACAATTTGAAAATAGAATTGAAATTATGTCTTATGGTGGATTACCAAAAGGTTTGAAACTTGAAGAATTTTATCAAGGAATAAGTAAGCCAAGAAATCCAAAATTAATGAAAATATTTATAGATCTTAATTTGGCAGAACAAACCGGACATGGCGTTCCACTTATTCTAAAAAAATATGGAAAAATGGCAATTGAAGTAGAAAAAAACTATATCAGGATAACAATACCTTTTAACAAGGAATTGCTACAAAAAAATAACAATTATGAAATTAAAGAAATAAAAGACAAATTAAACAAAATCGAAAATCAAATATTCGAATTAATTCAAAAAAATGAAGAATATACAGCTTATGAATTGTCAAACGAATTAGAAGTTTCTAAAAGAACAATAGAAAGAGCTTTAGCATCTTTAAAAAACAAACTAATTTTAAAAAGAATTGGTTCAAGAAAAACAGGAAAATGATCTGTTTTAGGAATAAAACCAGAAAATTAGAAAAAGGAATACAATTAAATCATGTACAAAAAATATTTATATCCAAAAATTGAACCTTATCAAAAAGGTTATTTAAAAGTAGATGATATTTATCAAATTTATTATGAAGTTAGTGGCAATCCACAAGGTCAACCAATAGTTTATGTTCATGGTGGTCCTGGTGGAGGAACTAGTGAAGTATGTAGAAGATATTTTGACCCAAAATACTATAAAATAGTTTTATTTGACCAAAGAGGTTGCGGAAAAAGTACTCCAAGTTTAGAGTTAAAAAATAATACAACTTGAGATTTAATCAATGATATGGAAGCCATTAGAAAAGAATTAAAAATAGATAAATGAATCCTTTTTGGTGGCTCATGGGGCACTACTTTATCATTATCTTATGCAATTAATCATTCAGATAAAGTTGATAAACTTATTTTAAGAAGTATATTTTTGGGACGTCAAAGTGACATCGACTGATTATATCAAGAAGGAGCTAGCTACTTTAAACCTGAAGCTTATGAAAGATACACATCATTTTTAAGTGATAAAGAAAGAAAAAATATAGTTAATGCTTATTATAAACATATACATTCAACAAATAAAGAATTAAGACATAAAGCATTAATTGAATGAACGAGATGAGAATCATCTTTAGTGTTGCTTAAAGAACAACCATTTAAAGAACCTAAAAAACCAAAGTGAATATATGAAATTTCATTAATTGAAAATTATTATTTCTATAATAAATGTTTCTTCGAAGAAAACTATATTTTAAATAATATTAACAAAATTAAAGATGTTGAAACTTACATTGTTCACGGTCAATTTGATTTGGACTGTCGCCCAAGTGGTGCTTATGAACTTCATCAAAAACTAAATAATTCGCATTTAATTATGGTTGAAAAAGCAGCTCATTCACAAAGAGAAGTTAATATAACTAAGGCTTTAGTTAAAATTACAGATCAAATTAGAAAAAAATAGTTTTATTTTTATATTTTAAGAAATTTTGGATTATCTATATTTTCTATAAAATAATCTTTTCCATAAGACAAATTAAGAATTGAATTATTATTAAATAAACTTAAAACTATTTTAGAAAAAATGAAAAGTAAAAATGGAGTAAAAGCATCTGATTTAAATTTAGAATAAAAAAGCCACATCATAGATGTAGCTAAATATGTATAACCCTTTACTTCCATATTTGAAGTTTTTAAATAATGCTTTAATTTTTATGAATAATTTTTACACAAACAATAAACATTAAAAATATATTAATAAAATAATAAGCACTAACTAAAACTATTAAACACTTTCAATAACAATAAATCTAAATTGTAATTTTAAGTATAAATAGCATTATTAAGGAAAGATAAATTATTATATTTCAATTAATGTTTCAATATTAGCTTTATCAATAGCAACTTGCATTGTTGCTATTTCTTGTTTTAATTTTAAATTAGTATTTTTTAATTCGTTAATATCATATAAATATTCAATATATTCAATTTGACCATCGTTGGTAGTTTTTCTTGTTTTTTGCTTTTTAGAACATAACAAATCAAATCTTTGAACTAAAGAAGATTTTTGTGCTAATAAAATTAAAAGTTCAGAAATACTTAATCCTTTATAACTAGTTATTGTTTTGATGTTTGCTTTTTGAACAATAATTTTAAGTCTTAAAATTTCTTGATTAATTTCTTGACGTTTTTTATTAGTTTTATTAAAATTATACGATGATTTTTCAACTGTTTCATCTTTTAAATATATAACATAATTATCTTTATCTTCTTCTTGATTAAGAACAATTAATTCGTCCTCAAGAATTTTAATTCTCTTGATAATTTCTTTGATCGATAGTTTCACATTTGCTCCTTTATATATTATTATGATATAACATTTTCTATATTATGCAAACAAAATGAATTAGGTTATTAAAGTTGAAAGTTATAAAAAAAGTAACATTTTTTTGCTAATCTGCGATAATTTAAGCATGACAACAAATACTAATAAAAATTCTTTAACAACAACCATTCAATTAATATTAGAAGATGGGACATTAGACGGGATTTTGTATCTCAAAACTTTAAGATGGCCTCTAGGAGGAATTATGTTGGTAAGTCCTCGCGATAAGGTTACTAAATTATTGGACGAATATGAAGAATGCAATAATTGAGGAATATATTTATTGATTGCAAAAGATCAAGTTTATGTAGGACAATCTTCAGAATTGAAACAAAGAATTGAACAACATTTATTAGGAAAAGATTGATGAGAAAAAGTTTTCTTATTTACTACATCAAATAATTCATTAGACAAAAGTGGAATCGATTATTTAGAATCAAAACTAATTGATAAGGCAAAAGCGAATAATAAATTAAGTTCAGATAATAAAAAAAATGGAAACAAAACTAATATTGATAGATTCCGAGAAACAGAATTAAAAGAATATTTAAATGAATTAATTTTCTTATTAGACTTTATTAATATTGATGTTTTTAACGATAATACAAACAAGAAAAATAATTTAGAAAATGTAAATTTATCAAATATTATTAAACCCAAATCAGAAGAAGAAATTAGAAGTCGAAGTAAACAGGAAATATTTTCTTATATTGAAACAAAAACAAAAATTGATTTAAAAAATAATACTTACTATAGTAAATATTATGATCGTAAAAATTATTATTGATTTACTTTAAAAAATAATTCAGTTAATAGTGATTTAAAATTAGTGCTAAATGATATAAATTCTCAAGAAATAACAATTTTGCAAATTCCAGCCAAAAAATTTGAAATTTCAGATACTAAAGGAACGAACAAATTTCAAAAAACTAGAAGTGATAATAAATTTGATTTATATATAAATAATGTTTTATTCGAAAAACAAAATAAAATTGACTTTACAGAATATGTAATAGATAAAATTCGTTATTCTTCCTCATAAGAATACATTTGAACCACTCTATGTGCTTTTTTTAATAAAAAAGTGTCCAACTAAGAACACTTAATTTACTCTAAAGAATCTAAGTCAACTTTAGTAACTTCAATTCCTTCAACAATTGTTTTTTGATTTTGAATATCAGAATTGTTTTTATCTTTATCGCTAAATATTTTATGTAAACCAACAACATATTTATAGAAAACATAATTAATAATTACTGTTGCAAAGATTAAGAATAAGATAGCTAAAATAATAAAAGCAAAATGAACATAAACATTTAAATCCTTAGTAAATACCACTTGTGCATATTTGAATAAAGCTGTTGAACTTATTTCGATAGGGAATGTAAAACAAGCTCAAGTGAAATAAAATTTTTTAGATTTTATTGAGATAGCTAAAGAATAAAAATATGCAGCTCAACCAACTAAAATAAATAAGAATAATATTCCTGTAAAAGTATATAAAATTACATAATTATTTAAAATATTTGCGTTTGGATTCTTAGGATCAAATAAAGTTAAAAATGCAAAACTTAATACCATTGCTGGAGCACAAAAAATTGCTAAAGATGGAATATCTTGATGATTTTTGTGGCCTACAAAAGCGACCTTAAATGCTACTAAAATAAAGATTAAAAAGCAGAATAATAAACCTATAAATCAGAGTATTTGGTAAAAAAGTAAGGGTATTATATTTTGATAATTATTAGCTCCAAAAGTAAGGATTACAAAACCAACTGTTGGAATAAATCAAGAAGCAAAAGCTTCATCATATTTAAAATCATGTTGAACTATAATTAATACTATAAAAGTTATTAAATAAGCTATTTGTAATATAAAAGCAATGAATGAAAATACCAATGGAATATATAACCATTTTTTAGCTTCACTAGCTTCTAAATAAGTATTTAATATTGAGGCTATAAAAGTAAAAATAGAAATAAATGTCATTAATAAAGTGGGTATAAATGCTGCTGTATTTGGAGCTTTTGCTTCTTTATTAAATACGTGCCTTGCTTTAATAAATCTTAAGCAAACAATAAAAGCTAAAGCAAAAGCAATTAACATCCAAAAAGCTTGAATGCCAATTGAAACATTATTAATTAATTTTCCATTGCTTGAGTTTTTAAATTCATTAAAAGCCCCATTATAAGCACCAGCTAAACCACTTATGCCAAGTGCAAGTCCTCCTAAACCAAGAGGTACTTTTTCTAATTTATTTTTAAAAGTCATGAATTAATTTTATATTAAAATAAAATTCTAGTTAGCATTTTTGAAATTTTTCAATGTTTCATTATCAAATTCAAAAACTTTATAAATTTGCTCTCACATAACTTTTGATCTACTTATCATTATTTGTTTTTGTTCGTCTAAATTTTCTCAATGCTTTTGAATAAATTCTTTATCCTCTAGGTTATGTTTTTCGATTGTTTTATCCTCTAGGTTATGTTCTTCGTTTTCTTCATCCTTTAGGTTTTGTTTTTTGGTATTGTAATCAAATTCTAATGATAATAATTTATCAAATATATAATCGCCAAATTTATCACTAAATTTCTTATTTCCTTTTGAACTATTTGTTTTTTTGCTAGCTATATGGGCATTTCCTAAAAGGTTTAGATATTTTCTTTCTTGGTCTACTTCTCCACTTTTATTTCTGAAAACTGGTGTTAAATGTTCTCAAGATAAATTTGTTCAATCTTTAAAATAAGAAGATTGTTCATCATTTTCAAAATAGTGTAGATTTTTATTATTTTTTCCTATACTATAATTTATTAAAAGTAATAAAGAAACTGGATCGTCTGAAGATGCTAATAAATCTGTTTTCTTATTTAACAAATCTTGTTCCATCTTTTTATTTATTTCATCAATTTCAGCATATTTTCTTAAAAATACAAAATTATTGTATGCTAATTTATCAAAGTTTGTACAGTCATCATTTAAACTTTGAACTATTTTTTTAATTGGCATTGTTAGTGATTCACCTTTAAATGAAATAAATTTGTTAAAGAATCTAATTCTAGCAACTTTATAAACTACTTTTGATAATCTTCTTAGTAAACTAATTCTCGATTCTTTGACCAAATTGACATTAAATAAGTTCATTTTGTCAAAAATAGATCAAAGTAAAGGGATATAAATTGTTTTGCCTTTTCCTATGATAAAGTTAACTAAAAACTTCATATTTTCAAGATTGTCATAATCTCAATCATATTTATTTTTAGAATTTCTAATCAATTTGTTTTTAGCAAAATAACTAAAAATTTCAACCGTTTCACATAATTTGTCGATTAGTTCTGAATAATTAGAATATTTTTTAGATAATTTTCTTCAAACTTCTACAAAAATATTGTAGGTTAAATTGTTTGATAATTCATTTGAATTAATTGATACGTTATTAATAGTTGCCATTGAGTTAACGTAATTATCAAACAATTTTGAATCCAAGACAAGTGTTTTTCTTTCACTTTTACTAATAAATAATGGTTTAAAGAAATTGTCAAATTTGCTAATGTTTGATTCTACATTTGAACTTTTTTCACTTAGTTGACCATAAACAAAATTACGAACTAAATCGTATGACGTTAATGGTTTTGAATACAAGTTAATTCTTTGAAATAATTCATAGTTTTTAGGAGTTTGTTCTTGGTTTGTGGTAATTGTTGTTAAAAATGTTTTTTGATAAAATGCTTGAACAAAATTTTTAAATTTTTCTTCTGCATCGTTAATGTTTGTAAAAAAGTAATCGTATAACTTCTTAGTAATAGCTTTTAAATTTTTATAAATATTAGTTTTATAAAATTCTGAATTTTTACCTTCGGCTGCATTTCAATTATCACCTAAAGCGATGCTAAGAGAATTGTATGAATCACTTTCACTAATATTGTTATAAATACTTGTTATTTTTCTAGGCAATAAACTATTAGAATTGTCTTTTATATCAATGTAATATCTAAACATATTTTCGACAGGATCTTTTGGTAAAAATAATTTCAAATTTTTAGCATGTAAAAACAATTTATATAAAGAATATGCAATTAATATTAATGTCACTGTTCTTTGTTGTCCATCGATTATTTCTTTAACCTGATTATTTGCTGTCTTTTCCCATCAATAAACAATTCCTAAATTTAAAATTTCTTTTTGATTGGAATTATTAGATGAAATATTAATTAAATCTCCAACTATTCTTAAAACGTATTCTTCATTTCATACGTAACTTCTTTGAAATACTGGTATAGATATCTTCATATTTAAAAGTTTATTAAAATATGATTGAATATTTAATACATCAAAAGAAGGAGCTAATTCTTTATAAATTGCTTCATTATCATCAATATAAAATTTATTTCTATCCAAATCTTTTGTAACAATTGCTTCTTCATTTGTATATAAAATATTACTTATATCATCATCTTTTTTAGCGTTTAAAATGTTTAATAATTCATCCATATTTTCATTAAAAGAATTTAAATTTGTGTTTTCATTATTTAATTCAATTTTGTTTATTAATTCCTTATAATAAGACAAATCTCTTTCATTTAATTCTGATCATTTTGAAAAAGGATTATTTCTTTTTTTGTTTTTTGAAAAAGAAAGTCAGCTAAAAATAATTGAGTTGATTATTGATTTCTCATCAAAATTTCCTAATTCAACTTCTTCATATTCTACGTTATTGTCTTCAAATTCTTCAATTCATTTATTTAATTTTTTAATTAAATTTTGTTTTAATTTTTTGTTAACATTTCGCAAAATATATTTATATACAAATTTTTCCAAATCTACTTTTTTATAGTTTAATTCGTTTTCTAAGTTACTTCCAAAATAAGCTTTTAATTCAATAATAAAATCTTCGTCGAATTTGTAATAATTTTCATGAAAATTATCTAATATTTTTAATTCTTCTAAAACTTCCATTGTTTGTCTTACTGTTGAAGCGCTATAGAATTCTTTTTCTTTTTCGCCTTCATCATAAAGAAAAATAAACTTTCTATCATACTCATATAGACAAACACTTAATTTATTGTTACTTCTATCAACTAATTCAAATTTAACATCTTTTATATCTGCAAAAGCATTAAAAATAATGTCTCAATCTCATTTTAAAATTGTTCTTTTAATATAGCTTTCAACTGATGTTCCAATTTTTCAAAAATTAAAAAACTTTTTTTCTTTTAAAAATTCTATATGTTTACTACTAATCATTTTGACCTCCATTATATTCTTCGATTTTTGCTATTAACTGAAATACACTTTTTATAGCTGGCACACAAATTGAATTGCCTGCTTGACGATAAAGAGCTTCTTTTGTTATTATGTTTTTTTTCTTAAATTCATTAACTTTTTCAAAATCTTTATCTTCAAATCCCATAATTTTGTAAGCTTCTCTATTTGTTACAAATCTTTTGTTATAAAAACCTTTTAAATTAGCATCATAATCTAAAACCCCAACATTAGGATGACGATCTTGTTTGGTTGTCAATGTGTTAATACGACATTCATCAATCTTTTGATTCTTATAAATAGTTGAATAATTTTTATTTTCAAAAGCCATTCTTTCGCGACTTTGACTTTTTTTGATTAAAGCTTCTTTTGATTCTTCTTCTTTTTTATTTTCTAAATCGAAAATTTTTGCAAATTCTTTTCATTTATTTTTAATCTCTCTTTCTTTGCCAAAATTAAGTAACTCATCTAAATAAGATTCATCATTTTCTTTAAAAGGAACTTTTAAATCATTTCTAACACTTAGTGCAAAAACTCTTTTACGATGTTGAAGTGAACCATGATCTTTGCCGTTTAATACTGCTGTAAAAGTTCTATATCCAAGTTTATTTAATTTCTTTTTTCATTCGTTATATTCTTCTTTATATTTAGTCACTAATTGTTGGACATTTTCAAGTAACAAATATTTTGGTTTGTTCTCTAATAATTCAATAACGCGTGCTATTTGTCAAACTAAATGACTAGTTGAATTGTTGTTTTTAATTCCTAACGAACGACCCATATTAGCACTTGAAAGACCTTGGCAAGGGAATGAATAAGTTATTAAATCGAAATGTAACTGATCTAATTGTTCTTTAGTAATCTTTGTAATATCATTAAAATTATTTGATATTAGATTTGCAGCTACTAGGGCTTTTTTAAGTTCATAACTTTTGTTTTTTAAGCTACTAATAGCTTTTTTACTAGTCATACTAAAAGTTCTAGACTTTAAATATTCATTAATTTTTGTTTCATCTTCTAATTTATTTGAATTCAGAATTTTTTCTATTTTTGTTTCATCAAGGTTGTGATGAATAGCTGCATAAGCAATGGTTGCTTGTATATCTCAATCGCATGTTGCAACGATTTCAAAATTTACTTCTTTTTGTTTTTTATTTAATCAAGTAATGGCTTTGTGTTGAGCACCAATACCAGAGAATGTTTCAAATATTCTTATTTTTTGCATAATAAAAAATACCTGGGGAAGGTATTAATCATTTGATAAAAAAACAAAAAGAGAGCAATTAAAAATTGCTATTTTAAATAGATCAGAGAGAGAGAGAGAGAGTAAGGCATATTCTTTTCCTTTCAAAAAAATATCTTTTGTTAGATTAATCTATTCTTGTTTGAATATATTAATTATACTATATTTTGTTTGTTTTAGAATTAAGAAATTAATCTAAAAATAAATAAAAAAGGTACATTGATTTTTACCAAAGTGCCTTTTTTATTATTAAATTAGTAAAATTATTTCTTGTTTTCTTCTAAGTAAAGTTTAAGTGAAGCAGCTCTTGCAGCTTCTCAAATAATTTCCGAATAAGTTGGGTGTGGGTGAATTGTGTAAGCTAATTCTTTGACACTAACTTCTTGTTCCATAGCAAGTGCTATTTCCGAAATATAGTCAGTTGCATGAGCTCCAATAATGTGAGCTCCTAATATTTGCCCATATTCACGATCAACAACTAATTTAACAAAACCAGAAGTTTGTTTGGTTGCAATAGCTTTTCCTAAATAAGAGAACATGTATTTTGAAGTAAAAATATTGTATCCTTTATTTTTTGCTTCTTCTTCAGTTAAACCAATGAAAGCAATTTCAGGGTTTGTATAAATACATCCTGGAACTGGTTTATTGTGATAACGAACATTTTTTTCACCTAAAATGTTACCAACAGCAATTAAAGCATGTTGATATGCAACGTGAGCTAACATGTTTTGTCCAGTTAAATCTCCAATTGCATAAACGCCTTTAACATTTGTTCTCATTTTTTCATCAACTAGAACTTCCTCTCTAACTCCGACATTAACTCCAACTTCTGCAATACCTTTACTTGATGGAATACGTCCAACAGCAATCAAATAAACATCAGCTTTAATTTTTCTTTCTTTACCACCAATTTCGTAAAGAAGTTCTTTTTTATTATTTAAACCTGTTGAAGTTGCATTGTAAATAATTTGAACCCCATGCTTTTCTAGATGTTTAAGAATTTCATTTGTTACATCATGATCAATACCAGGAAGTAAGTGGTTTTGGTTTTGAATAATAGTTACTTTAGCACCTGCTGAAGCAAATACTTGAGCAAATTCAACGCCAACAACACCACCACCAACAATAACTACCGATCCAGGCAATTTTGAATCATAGTTAATTGCTTCGCGGCTTGAAAGAACATAATTATTTTCATATCCAACCTTAAATCCAGGTAAAGCACGCAATCTACGTGAGTGACTGCCTGTGGCTAAAATAATATTTGTGCCACGATATACTTTACCATTTATTTTAATTTCGTGAGCTCCAACAAATTCTGCTTCGCCTTCTTCTATTTTACATTTAGAAGCAATCATTAATTTTTTAACACTACTTGAAATTTGATCTACTACGCCTTTTTTACGTTCATGCATTTTGACTCATGTTTTTTGTAGATCTATCTTTAATTTATCTAAGTTCCCAACAATACCATAACCAGCAGCATCGGTTAAGGTTTCTAAAACATCAGTTGATTTAAGCATTGCTTTAGTAGGAATACATCCTATATTAAGACATACACCACCTCAGAATTCTTTTTCAACAATTAATACTTTTTTGCCTGCTTTGCCTAATTCTTCAGCGGCTAAATATCCTCCAGGACCACTACCTACAATAATTACATCAAATTCTTCATCAACTTTGCCTTCGTATTTTTTACCTTCAGAAGTAAATGAAACACCATTGCTTTCTTCTTCTTGGTTAGCTTCATAAGTTGAAGCTTGAGGTGCAACTGGTTGAGGAGTTTCTTTTGCGGGTGAATTTAGTGAACTGAAATCTAGATTTATTAATTCACTTGAATTTGGAGCATCACCTACTAAACATTTTTCTTCTTCTTTATTGGAACTTGGTGCAGATGAAACTGAAGAAGAAGTAGGATTTTTACTTCCTGCATCAATAACAATAACTTCTTGTCCTACATGAATTACATCACCTGTTTTCATTTTAATTTCTTTGATTATTCCATCTACTGGAGAAGGAATTTCAGAAGCAACTTTATCAGTTTCTACTGAGAATAATGTGTCGCCTTCTTTTACTTTATCACCTATTTTAATAGGAATTTCACCAACTGTTCCTTCGTGTAAACCTTCACCAATGTCAGCAAATTTAAAAACATAAAAGCTTGTAGATGATGAAGTAGCAGGTGTACTTTTCGATTCTTCAACTTTAGTAGCAGGAGCTGCAGCTACTGAGCCACCTTTGCCATCATCAATTACAAATATTTCTTGTCCTACATGAATTACATCACCAGTCTTCATTCTAATTTCTTTGATTATTCCATCTACTGGAGAAGGAATTTCAGAAGCAACTTTATCAGTTTCTACTGAAAAAAGTGTATCTCCTTCTTTAATCTTGTCACCAACTTTAACACTGATTTCACCAACTGTTCCTTCGTGTAATCCTTCACCTATATCAGCAAATTTAAATACGTACATATTAATACACTCCTAAAACATCTGGTTTTTCTAATAATTCTTTGATTCTTGAAGCGAATCTTCCAATTACGGCACCATCAATTCAACGGTGATCAGCAGCGACAGTTAGGTACATAACTTTTCCAGGTACAACTTGTCCATTTTTAACTACAGGCTTGTCAATAATAGCACCAACACCACCAATAGCTAATTCTGGATAGTTAATAACAGGAACTCCAAAAAGTGATCCAACTGAACCATAGTTAGTAATAGTAAAACCGGCACCTTTCATTTCAGCAGGTTTAATTGTTCTATTTCTTGCAGCGCCTGCAAGTCTAGTTACTTCACTAGCAATATTAACAATACTTAAATTATCAGCATTATTAATAACAGGAACCATTAAACCGGCTTCAGTATCAACAGCAATACCAACATTAATTACACCAGGGTAATCTAAAGTTTGATTTGCTTCATTATATTTAGCAGTGAATACTGGAAAATCTCTTAAAGCAATAGCAGCTGCTTTAATAATATAAGGTAAGAATGTAATTTTTACATTATCACTTTTAAGTACAATATCTTTGATTCTTGAACGTAAGTCCCAAAGAGCAGTCATATCAATTTCATGAACTAAATTTGTATAAGCAACATTACTTCATGAGTTAGTCATAGCTTTAGAAATTGCTTTTCTAATTGGAGTAATAGCTTCTGAGTGAGCTGAACGATCAATGGCAGGACGAGGTGCTGGAGTAGCTTTAGGAGCTACTGCACTAGGTTGAGTAATTACTTTATTATCAGCTACTGGAGCTGGTGCAAAGTTTGAATGAGCAGCACCTGAAATAGGACTGTTTTTAAATTTTTGAATATCTTCTATAAGAATACGGCCATCAATTCCTGAACCTGGAACTAAGTTAATATCAATTCCAAGTTTAGCAGCCATAGCTCTTGCAATAGGGGTTGATTTAATTTTCATTATTTCTCCTTATAAGACTATAAAACAAGTCTTTTAGTAAATTAAATGGTCTTTAAATATTAAAATTTAAATTCCATAACTTCTTTTATTTTTGCAAGAATTTTGTCTTCATTGATGCACATTCATGCTTCGCCTTTTGCAAGAGGAACAGTTACATCATAACCTGTACATCTTGTCATAGGTGCTTTAAGATATTCAAATGCTTTTTCATTAACTCTAGCCATAATTTCAGAAGAAACTGAAAATGATTTAACAGCTTCATGAACTACTAATAAACGTCCTGTTTTCTTAACACTTTCAACAATAGTTTTAGTATCAAGAGGTTTAATTGTTCTTAAATCAATTAATTCAATTGAAGCGTTAGGATTGATTTCTTTATATTTTTTAACAGCATTAATTGAGTCAAATACTTGAGCTCCATAAGTTACTAAAGTTAAGTCATTACCTTGAGTTAAGACATTAGCTTTTCCAATTTCAACTGTATATTCGCCAGCTGGAACTTCTTGTTTCCCAGCACGATAAATTTTCTTATTTTCTAAGAATACTACTGGATCTGGATCATTTAAAGCTGCTAATAATAAACCTTTAACATCATAAGGAAAGGCTGGCATAACAACTTTAACACCTGGAATGTGAGCATAAATAGCTTCTAAAGCTTCTGAGTGATGTTCCATAGCTTTAATTCCACCACCCATAGGCATTCTTAAAATCATGGGCACGGTGAAACGTCCTCTTGAACGGTTTCTTCATCTAGCTGCATGAGTAAATAATTGTTGCATAGCTGGATAACTAAAACCTTGGAATTGAATTTCGGCAATAGGTCTAAGACCTGCTACTGCAGCACCAATTGCGGTACCAGCAATTGTTGCTTCTGAAATAGGGGTATCAAATACTCTACTTTTACCATATTTTTTTTGTAAGCCTTCTGTAGCTCTGAAAACTCCACCTTCAACACCAGCATCTTCACCATAACAAACTACACGGGGGTCTTTAGCCATAGCAATGTCTAAAGCATTATTAACTGCTTGAACATTATTTAAAGTTAATTTTTCTTCAGCCATGATTATTTGCCTCCTTTAGCTTGTCAGAATTTAATAGCTTCTTCTCTTTGCTCTAATAAATCATCATCAAGCTTTTCATATGTATAGTCAAAAATATCTTCTAATTTGGTATTGATGTCTTTCAAGCTTTCTTCATAAGTTTTCTTAACTTGTTCAAGAGCATCGGCTCAAATCTTTTCTTTTTCTTTTTCAGTAATGATTTTTTTGTCTTTCATATATCTTTCGATGCGATGCATTGGTTCTCATTTTTCATTTTCTTTTTCGTCTTTTTCACTACGATAAAGTTTTGGATTGTCACTAGTTGTGTGAGGGCCTTGACGTCATGTTAAGAATTCAACAATAACTGGTTTGCTTTCTTTTCTAGCATAATCAATTGCTTCTTTAATTACTTCATAAGAAGCTAGAAGGTCATTACCGTCAACTCTAACACCAGCACAACCAAAAGCATGAGCTTTAGCTGCAATAGTAGCTGATCTACTTTCTTCTGAGTTAGGTGTTGAAATAGCTCATTGGTTATTGTTTACACAAAATACTGCGGGTCATTCTTGAACAGTTGCAATATTAACACCTTCGCTAAATTCTCCTTCAGTAGTTCCGCCATTTCCAATAAATGAAATAGCTACACCACCAGTTTTAAGTTGTTTTTGTGCATAAGCAATACCAGCACATTGTGAAATTTGAGTTGCAATAACAATATTAACCGGCATTACATTAACATCTTTAGGATGGTTGCATCCTCATTCTTGACCATTTCAGTAACGAATTAAGTCAATCATAGGAACGCCTAAACGAAGCATAGCAGCATTAGATCTAAAAGCTGGAACAAATCAGTCTTTCTTTTCCATAGCCATTGCTGTTGCAATTTGTAATGCTTCTTCACCTAAGTTAGGTGGAAATGTAAGCATACGTCCTTGACGTTGCAATTGCAACATGTATGTATCTTGTTGTCTAGATAGTACCATTCATTTGTAAGCTTCTAAAACTTCAGCATTACTTAGTGGTGCTTTGTAATTTTTTTCAATTACTTTACCATCTATGTCTAAATGTCTAATAATCTTATTTGGATCGTCCATAATTTTATTAACATCGACATATTTGTACTTTGTTTTCATAAAGATACTTCCTTTCTTGAGTCAAAATTAAGTCTTTTATAATTAAGACTATAAAAATTTTATTATTTATATGAAAATATTTCCAAAAATATATTTATATAGCATTCTTTTTGTATCTTTTATGGTAATTTTTTCATTTATTTTTCTTATTCTTGATTTTTTGAAAACTTTTTTGTTAATTTTGAAGCAAAAAGTGAAATTTATTTTTTTCATTTTAAACGCCAAAAATGATATAATGGCTTATAAAAGGTAGCAAAAAAAATGAAAAATATTGATTATAAAAGAATTTTTAATAAAGGTATCCCCACAAATATTATTAACTTGTTGACTGAAATATATGAATATAAAGGAAGACAAAACATTTATATCAATAACAAGAATTTAGAAATCCAAAAGCTTAAAAAACAAGCTTTAATTGACAGTGCAGTTTATTCAAATAAAATAGAAAACATCAATATAAGCAAAAAAAGAATTAAAGATATTTTGCTTGAAAATGATGAGTTGCAAAATGAAAATGAAGAAGATATTAAAGGTTATTTTGAATTATTAAATTCTCTATATAATCAAGAAACATTTGAATTATTAAACACTAATTTGATTCTTGATCTTCATTATCAATTGTTTCAAAACTCAAATACCAAAATGAGTGGGCGTTTTAAGTTAAATGATAATTTTATTTTAGAAACAACCTTAGAAGGTACTCAAGAAATAAAATTTATTCCTGTCAAAGCATTTTTTATTAGTGAATATATAAATAATTTATGTAATAAATATAATGAAGAATATAAAAGTAAGAAGATTAATTCATTGCTTCTAACAACAGCTTTTGTTTTGGATTTTATTTGCATTTCGCCTTTTAATCACGGCAATAGCAGAATGTCTAGAATTCTTTTAAATTGACTATTAAGTGTTGGTGATATTAAAGTCGTTCAGTATATAAGTTTAGAAAAAATCATTTTTGAAAACTTGGAAAAATATCAAAATGTTCAACAAAAAAGTTCATATAAATGACATGATAATTTGAATAACTATTATTACTTTATAGAGTTTATTTTAAAAATTGTTTTACAAGCTTATAAAGAATTTGAAAGCCGTTTTACAAGTTTAGAAAATAAAAAAATTGATGTAGCGGACAACATTTTAAAACTGATAGCTCAATATCAAAAAGTAAGTAAAAATGAGCTAATTAATTTACTACCTGAAAGCAGTAAAAGAACAATTGAAAGAAAACTAAATGTCTTATTAAAAGCTAAAAAAATACTTAAAATTAATCAAGGCAAAAATACTAAATACACTATAAAACAGCATTAAAAGAAAAAATGGGACTGTAAAAAATTTTGTGTAAAGTTCTAAAATCTTTTCTTGATAGACAATAAATTATCAAGAAAGGATTTTTTATTTATGAAAAGAAAAACAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAATTATAGAAATGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTTAACAAGAGATGTAAGTGATTTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGATAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATGGTGAAGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGAAACTGATCTGAAATTTCTACAATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTAATTTTGAAAATGAAGAATAATTTTTTCAAATTTTAAAAGCTTTGCTTCTTTGGCTTCAAAGAAGGCCTCTCCCTTAAATGAGTTAAGGGAGACGGGAGAGGGATTAAGATTTCTTGCATGGCAAGAACTTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTGCAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTTTATTGAAAAAAGGAGAGACTTTACACAAAATTCTAAACAGTCTCAATTTATCAAAAAACAATGATAATAATCTCGTTTTAGAACATTTAAAAACAACTGATTTTTCTTCAAATTTTATTATTCATTCAGATCATGGCTATCAATATTCATCAAATGATTATGTAAAATTCATATCATCAAAAAATGAAAAAATCTCAATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGATATAATAATGATAGATTTATGAAAAAATTAGATCTAAAAACACCCCAACAAATGTGGGATGTTTATAAGAACAATAAATTTATTGGAGTCTAATTTTTTTGTCCTAGTTTAATTTAAGAAAATGCGTTTTTTTAAAACTTACCACCCTCACTTTTGAAAAAAATACAAGAATTCGATTAAAAATAAATACTTTTTTAAAATAAATTATTTATTATAATTATTTTACTTATGCAGAATAAGAAAAAGGAAAATGGAATAATGATTGATACATATCAAACTATCTTTGAATTAAAAAATATTATAAAATATATTAAAAAAGATTTAAAGTTAAATAAAGTTTCTCTAAAAGCTTTCAAAGGCAAAATAAATGGAACTAATATAAAAAGGTGTGTTATAAGATTCTCTACGTTTTCTTATTTCGTAATTATTTTATCATAATCCATTTTGTAGCAAAGGAAAATACTGCATGTGAAGAGCATGCAGTTTCCTTTTGCGCGCACACGCGCTACTTGAATAGTACAAGATAAAGACACTACAAAGCTATTTACCATAGCGTGTTTTATCTTGAAATGATTTATTCATAGAATAAATCAAAAATTAATATTTTCATATTTTCTAGGCGCATGCACTTTTTTCGTTTTTGCCTAAATTCGCATGCACTTTTACATGCACTTTAACTTGTATATTTTTATTTTAAAAATATATTAATACTGCTTTTGCGCATGCACTTTGTTAAAAATACTGCTTAAATGAAAAATAAGTTTGCTTTTGTTTCCATATTTTTTGCAATGCTTAACTGTATTGTTTTTAGTGAAAATGTAGCAAATTTCTTTGAGCAAAACCTCTTTAAATCGTGCCCAACTACCGTTTCAGCACAGCCTCCAAAGTAGTTTTTAACTAGCACTGAGTTTCAAATATATTCTCTATTTTCTTTATAATATTTCTTGAACTTTTTAGCATTAGCATATACAGTTTTATTTTTCTCTTGCTTTGTTATTCACTCTAAAACACTGTCAAGTTCTTTCTCAAATTCATAAATTAATCCTTTAGAAAACAAGTTGTCTAACACGCGATAAACATCTTTATAATCCCTAAAAAATCTCTTATTACTTACATTAATTTTTTTGCTATATCCAAAAGCTTTAAACATATTTGCTTTGAAATGAAATGGATCATAAACTCTCGTTGATTTTAAATTATCAGCTATTGTAGTTATGAATTTTGCACCATCTCCAGAGACAATAATTTCACAATTTTCTTTATATAAACTGTTTAAACTTTCTCTAATTTTGTTTTCAAAATCTTCTTCACTATTGAAGTTTTTAAACTCACTTGCAAGTGGTAAAAACTGCATAATAGAGGTCTTATTTTCTATGGCTTTTACACTACTTCCGCTAAGCAAAGTATGTATAGTCGCCATACGCACTCTAACCTTTATTTTTCTACCTTTAAAAGACACTCTAATATAAGCATCATCAATATCTATTTGTATAGTCTTGTACTCTAGATTTTTATAGTCTTTTAAAGTCAAATTATTTAATACTTCTTTCTTTAATTGTTCCCTATGGTAATAATAAACGTGATAAGGAATAAGTAGCTTACTATTATCACCTTTTGAAGCGTATATATTGTTTTCAACACATTTTTCGACGCACTCTTTATAATATTTATTTCTACCGTTATCAGCTAATCCTTCACTCGGATAAATAACTAGTCTTTTGCCTGTAGTTTTATCTTTATACATGTAGTAAATAATTCTTACTGTCTCGCCCCAAATAAAAATATCTCTTTCTATTTTTCTTTCAACATTATATTTAATTTTACGCTCTTCGCTATTCTTAAAAACTTCTGCTTGAGCATTTCAATTGTCAGCTAAAGTTTTACTCATTTTGTTCTTCACAGCGCCCTCCATAATCAATTAAAAACGACGTTTTCACGCCGTTAATAATTTTATATGTTTTACTTCAGCTTCGTTTTGTCTTTGTCTGTTTTGCTTTCTTTTATTGGTATTTCCTTAGCTACTTTATCCTTAGCTTTTTCGATATTTTTATTATCGTATGGATCAATATAGCTTGATTCACTTTTGCTACTTTTAGATAAAAAACCTATATTTTCAAAAGAATATATATTAACGTTATCCTTCGTCTTATCTAAGTCTTTTCAATCATTATGAGCAATTTCTCTAATAAAATAATTACTAGTTTCTTTATCTAATTCATACTCAAAATCATTTTGATTAATTATATTTTTCTCTTTACCAATACATAATTTAATATTCTTTAAAGCTTCTTTTGTATATTTTGTATTAGAAGTGTTTGTGTTAATTAGGAATTGGGATTTTGAAAAATTATTAGCAAAATAGTTTTTTGTTGCACGCTTAACTTTATTACTATTATTAGACAATGTAGATAAAAGCTGATTATTTATTAACTCATCGTTAGTATATAAAACTAAATTGTGCTTTAAATTATTTGATATCTTTATTATATTTAAATCGGGAGGAATTGCATTTATACGGCGACTTAGGTCATCGACTATCAATGATTTATCATTTTTATTTAATACAGAAGTATCTTGATAAATTGCTTTATCTATATTACTTTCAAGCAATTCCTTATCAATAATAAATATATCCTTAGTAACTACTACACTTTCATGATTCTTTCAATATTCGTTGGTTTGATAAGAGCTATTATCAATTATTAAATTATATTTGCTAGAACTTTTTCTTGCCGTTTGAAGTCTTATACAATAATAAGTTTTATTTTTTGTATTTTCAAAAATTATCAAAGGATGAATTTTAAATTTTGTATCGATATTTAGATGAAAAATATCTTCTAATAAAGGATTTGTAATTTTTAATGGTCTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAAGAGGATTAAACCTCTTTTTAAGACGAACTAGAGTCTAAGGCGTATCATCAAGCGCTTAAAGATAAAATATGAGCTGCTTGCTTGTTCGATTACTCATATAACAAAATCCTTCTTAGACTATTGTTTAAATTATACTACAAATAAATAAAAAAATTAATAATTTTTCAAATCTTATTTGCATTGTTTACTCTTAAACAAATTGATAATCTTTTACTAAAAAATTATAAAAGGGTGGGCTTGAATTTTTCCGTTATTTAAAACTTTGCCATCAATAGGTAGTTTGAAAATATTTTCTAATAATGGGTTATTAATTTTTAAAGGTTTTCATAAATCTAAAGACATAATAAAATTATACAAAAAAAGAGGTATAAAACCTCTTTTTAAGACGACCCAGCCTAGAGTAATTGTAGCTACACGCTAAGCTTGCTACTGTTCGATTACTTACGTGTATCTCTCTAGGTTCATATGTCTACATTATATAGTAAAAAAACAATATTTTATAAAATTCTTAACTTCACCCATATTTTCTTAATAATGTTTTAGTTAAAAATATAGTGCCTAACATTTTTTTATTTTGCAAATAATTTATTAATTTCTTCATCGCTAAGAACAACCGGCTTAGCCTTAATTCTGTCTTTTTTATAGATTTGTATTTCTTTGAATTTCTTAATTGAAACTTGTTTCTTGCTCTTGCCAGCTTTAAAGCCTTTTTTATTCAAGTATCATCTAATACCATATATGCCACCAACAGCAAAACCTAAGGCAAGCATAACCGATACAATTCAAATTCAATAATTACTTTTATGGCTTGCTTTTGTTTTATCAACATCTAAATCTTCACCATTTGGCTTTTTATGCTTATCGTTTTGTATTTCTTTAAATCATAATTTATCATCAAAAGCATTATAATTTGCCTCAATATTATTCACCACTATAGCATAGGCAAAATTAATATTATTGCCTTCTTTAACTGGTAATAAAACAAAATACTTGCCTCTAGCTTTATAATCTAAGCTTTTATTAAAGTTATCGTTTTGTATTTCTTTAATTGCCTTTTCTAAAGCTTCGTGGTACTTCTTAAATAGCTCAGTATCATTTATTTGATACGGAATAGGAACTTTATGTCCTTCAATTTCTCAAGTATTATATTCAGCCATTACCTTTGCTTTAGTTTCTTCATAAACTTTTTTGAATTTTTCATATTTAGCTTCATTTAATTTATCAATATCAGCTACTAAACCAAGTAAATAGAATGAAGCATTTACAAGTGAAACTAAATAATCTTTTTTAAACTCGATACCTGAAGCAATCGTTATCTCATTTAACTTATTTATAATATCTTTGGTAATTTTCTCAGGTTCTTTTTGCGATATAACAATAGGATTTTTTACTAGTGTATCATTAAGTAGTGATAAATCAAATGCTGGTGCTTCAGTATCATTAATAATATGAATCTTAGTGCTTGAAATTAAATTAGCATTAATACCTTTTATCTTAATATCAATACCATCTTTAAACTTATTCCCCTGTACTAGTAAATCTAAAACGTTATCTAGTCCTTCTACTTTAATATCTTTATTCAATAACATTTCTATGCCAGTTTTGTTTTTGTATAAGTTGAATTGTTCTTCTATTAGCCCTATCAACTTATTTCTAATCTTTTCTTTTAATTCTTTTGAATCATTACTAATATTCTTTCCCATTTCAATTTCGAAGTGTTTTTGAATGTCTAGTGATGATAAATCAATAGGAGGATAGTACACGTGGTACGCACTATTTTTCAAATTAAATGTTATGCTGTGATTTATGTATTTACCCGTTCCACGCAAAGTAAAAGCTCATCCCTTACCGTCATTATCTGGTACATTAATATCTTTAATATAAACTTTTAAAGCCCTATCCAATCAACGCTTTTCGCTTTCTGGCGTATTTCAATTAGCTATTACCCAGCCACCATTAATTTCATTTTCAGTTATTCCAATTGCCTTTAATTTAGCACTATTAGCAATATTTCTTTTAACAAAATTAAGCACGATTTCTTTAACTTGTCTTCTATAATTTTCTTTAATAGCTTCATTAGTTTCATCTCCATAATTCAAGCCATTAAGATTTTCTAATTTACCTCAAGTTATTTTATCTTTAAAAATATTTTCATACTCGTTTCCTGTTTCTACATTAAACTTTGACGCTTGGTCTACAAAGTTTTTATATAGTGAAATCATCTTGTCATATCCTAATTTACCCAAGTCATCATTTTGGATACCACAAGCTTCATTTAAGTAATTTTGAAATAGTGTAGTTATTCAATTGCCACCATCTAAAAATGCTTCAAAATAATCTTCACTTTGATTATTAATTTTTTCTATCTTGTTTTCAATTGAATCCAAGAAAAAACTTTTTGGCTCGCTCTTTGCATCAATTAATACCAAATTAACATTTGATATTGATTTTTCATTTACAGTAAATAATAGCCAAATGCCTTCCTCAGACATATAACTATATTCACTTGTCCCATTATTTACCGGTATTTCAATTAGTTTTTGTTCTTTCCTACCAATCTCAACGTATTTGTCATTTTCTTTTCTAAACAACAAACACTTATAGTATCTTGAAGATGACTCTAAAACTTTATCAACCACCAAGTTTCTTAATGCCCCCTTACCAAGCACATTAGCTTCAGCATAGATTCCAAGATCTGCTTTATCTGTAGTTATTTTAATATTAGGATATACAAAACGAAGTTGTTCTTTTAATTCATCACTAAACAACTTATTTTTAATTCAAACAATCTGCTTTTTAGTTCCTGTTTCTTTATCAATACTTGGATCATAATTCTTATTTACTATTAATTTGCCTTCTTTATCCTTTAACTCTTTTCCCTTTGAATCAAGCATATATGGAGAAATAATTTGTTTTTGATCCATATTCTTTTCCGGCTCTCAAGCATAATATTTTATGTTTTGTTTAAACTGATTTCCAATTACTTCATAAATAACTACATACTTTTCTTCCGGCTCTCCACTATTATTTGAGCCTTTCTTGTATCTATAAACTTCAACAAGATATTCATTTTTTGCAACATCGCCTTCGCCATTACCGCTTCCCGAGTTTTTATCAAAAGGTATTTTAGATGCATCATTAACATTAACTCTTTCATCAATTAATTTCTTTTCAAATATTCTATTTAAAACACTAACCTTTTGCTTATTTACCATTAAAACTTCAGTTTCATCCTCTGAAGCTTTAAAAGAAATATTAATTGGCCCTCTAACTACATACCTTCCACCAAATCATGGCTTACCGACAAAATCTTTTTCAAGTTCTTTATTGCTTACCCTTTCAGGTTCGTCTCTTTTAATTAGATTTTTTAAATCGCCACCATTAGCTTTTGTCTTTGCTTCTATTTGACTTATACTTCCATCAGGATTAGTAATAGAAGTTGATATAGTTAACCGTGAAGCTAACGAAGCTCCACTAAGTCTTTTATACAAATCTGATTCTAAAATATTTACCTTTAATGAATCAAAAACTTTAAAATTACCTAAAACGATATCTTTTAAAACCCTTAAAAATTGATTCCACTTCCCAACAGCTTCAGCTTTAATGTTTTCTTTTGTTCAGTCTTCAATATTATTAAGAATCTTATCCAAGTCTTTATCGATAATTTCCTTATCATCAATCTTGACTGATAAAACCTTAATATAAACATCAATTTTGTTCTTTTCACCAACAGCTTCGTTTTTTATCTTGTAATCATATTTGTATTTAACTTTAATTCCACTAGCATACTCAAACTCATATTCTTTATCGAATATTTTGTTTAAATGTTCTATATTCGATTTGCCTCCATTGTCTTTAAAAACTCCATCAACTGATGTAGTGAAATCGCCCAAGGTATCGCTTAATAAATCTATAGAGTAGTGATTTTGGTCAATTTTCTTATTTACCCCATATTCAAATCTATTCAAACAATTATCACTTGAAACACACTCTTTTAAAGCCTCTATGAATCAGCCAACTTTAGCATATTCTAGATTATTTAATACTTTATTATTATAGTACCTTACATCATACATTCCTTCAATTTCACAATCAAAATCATGAAGCCCCCACAAAAACATTTTATTTTTATCATCAGTTAGCCCACTATACACCCTTTTATCTTTATCAGTTTCAAGATTATTTAAAAACTCTAATGTTTTTTGAGAAACTTTATATTCAGCAAACAGGTTAGTAAAACTAGCACCTTTATGTTCTTCCATAAAGTTGTTTTTGTCTCATTTTATTAATTGTTCTATAAAATTAGAAGCAAAAATTACTCAATTCTCTTTTTCACGAGGATGCAAATAATTTAGATAATATTTATCATCGTATCAAAACCAGCCATAACTATTATGATTACTAGGATTTGTGTAATAGGCTTTTTCATAACGCACTTCAAAAGAGCCTAAAGTTGGCCTAGAAACTATTGACGTAATTTTATTTTTTACTGGGCCAGTTTTATGATAACCAACCCATTGGTCCTGTGCATACAATAATCCTTTATCACATAGTTGTGACCATTTTACTTGATACCTATTTTGTAGTCTTTCATTTTTAGCAATTTCATCATCTTCCATACTAGTAAAATTGGAAAATAAACTATTAGTATCAATCTTATGTTTTTCTTCAGGAGCTTTAAAATCTTTTCCATAACCTAGAAGTTTTTTATTAGTATTTAACGATAAATTAAGTACTGACGGAATCGCAATACTCAATACACTCAATGGAATTAATTTTAACATCTTTTTCTTATTCATTTTTTCCTCATTCTTCGCCCGTGCCTCCGCGTGCATATTAGTTATGCCCGTGTGCGCACGTGTGCATGTTTAAAGACTATTTTATTTTTTAAAAAACTCACTTAAATCAAAATCACCAAAATCTTCATCCTCTTTAGCATTATTTAATTCTGAAGCTTCAAGTTTTGCATTTTCTTTTTCAAACACTTTTTCAATTTCATTGTCTTCGAATTGTTCTGCAAACTTGCTTAATGAATTGTCTTCATTTCTTACCTTTTTAATTACCTTCTTATACCATGCAGAAGCCGATTCATTCATTCTTTCACGCAAACGCTTTGCATATTCTGGCTCTTTAAGCAAATTATTGTTAGGTTCATCTAACATTCCTTTATCGCTTATAAGAGCATTTAACAACATATTGACTTTTAAATCAAGCAAAACCAAATCAATATTTTGCTTGTTAATTTCATTTATAATCTTGCGATAAAATGGAGTCAAAGACGCTCAAGATACTTTTCTTAATGCATAAAATAAATCATTTTTGAAATCTTTAGCTATTTCCTCTTTCTCTTGTTTAATTAAAGCGTTGCGAATTATATCTTTAAGCTCTACATACGGAGTAGAGCCACTTTGTTTAATTCTTTTTACCCATCTTTCGTATAACGCCACATCATCTGGTTCACTAAGACGCGTAGAAATACATTTAGAGCCGTTTTTTGAGGCTCTAGAGCTTTTTACATATAATTCACCTTTTTTATTTTCTTGACTAATTTCTCATGTTTTCATCTTCCCCCTTTTCAAAGAATAATTCCTTTTCCTTTGGATTGTAATGTAGATTAATTTTGTATCTAAGTGCATTACTCATACTTGCTAAAACTTTCCCCACTCTTCTTCCTGAAATAAACTTAACTTCAGAATCTAGTAATGGAGCTTCAGCCGTTTTGTATAACTTTTTAACCGCCTCAATATCATCTCCTACTAACCCTAGAAAAAATGAATATTGACAGTTCCCCGTAATCTTTTTAGCAATTTGTTCAACTGCCGTGCTTGAAGCATAATCACTAGGATTTTGCGTGGTATGTATTGTCCCGCAATCAAGCTTACGCACAAGCTTTGTAGTATCTGATATATAGTTCAAAGTAGTTATATTCTTTTCACTAATATACAAGTGTTCTTCATCAATACAAAATGAACAATAACGTGTTAACTTTTGTATTTCTTTCTTGCTAATTAAACGAATCTTATTTTTTTCTTTATATTCGTTAATTCTTTTCTTGTTATTAAAAGCATTATCAAAGATAAAGCCATTCACCAAGCTAATTAAAACCATAATGCCAATTCTTGCATTTACGCTACCTTCAGTATCGATTAAATTACTTGTTTTAAAAACAACCAAATCGTTTTCAATATTGATATTGGTGTGACCATTATATAACGCACCCAAAGTTCCATATCTTCCAAATTTACCCTCTAATAATTCAGCCATATCTAAAACCTTTTTAGTATAGCCTTCTTTATCGTGATGGTCTTTAGGAGCTACAAACTTTTTCATTTGCTTAATCAAATCATCCATAGTTGGTCATCCATCGTTTTTGAGTTCTTTAACTTCCTTTAATCTATAAATGCCCCAACTTTGATATAACTTAATAATTTCAAGTTGCAAAATGTTCTTCTTCATTTCATTCATATCATCAAAAATGAAACTAAAGAACTTTTCTAACCAAAGCAAATGATTATTAATTAAAGTATTGAAATTAACATTGGCATCATCTTCTTCACGCGCAATAAATGATTTGACTTGTAATGGATTAATAACCGTTCCATCTCCAGAACCTAAATCAACTATTTGGCCTCCCATTTTATTTGCCCATTTAACATATTCAGCTTGCGGATCAATAATAATTGCTATATTCCCCGCAGCCAGTGATGAAGTAAGAATCTTCTTGGTTAAAGTACTTTTGCCCATTCCTGAAGTTCCCAATATCATCACATTATGATTTGCTCTACTTGAAGTCAAGCTAAACATATTTCATACAATAGGACTATCATTTCCTCCCACTTCATTTCCAAGCAACAAGTTGTTTCCGTCATTAAGTGGCAGATTTTTCAATGGATAGCCTATTGCGTTATTTATACTTGTGATTTGATAGTACTCTCTATCTAATTTGCTTAATGGATAATTAGACATATCTAAAAATCCTTCAAATTGTCTAAAGGTCAAATCATTAATTCCAAAATTAGATCTTGTAAAACCATTCTTAATTTTCAAATTATTTTGCTTCAATTCTTCAGCATTTTCCGCTTCAGCTAAAACAAAAATATTTGAATCAAATAACTTAAAATTATCCGCTTGTATTTGGTCAATCATAACCGCAATCGATTCTTCCTCTAATGCTGAACCGAATCTATTAATAATTGAATTATCCAAATTACCAGCGTCCATATTTTTCTTATTCGCTTTATCGATTATCTTATTTACAACATTTGGATCGTTATATGGAAAATTGTTTCACACAACTGTTCCCGATACATCGAATAACTTCTTTAATCACTCGTTATTCAAGCGTATTGGAATGTTCCCAGCACATTTAACGCTATACAACTTATCATCAATTTTTAATGAATTGCTTTTAAACTCCACCTTTTCATACTTGAACAAGCGGTCCAAACTATATTCACTTTCGTAAATATCACTTTCATTTAAAAACGTTTCAATATCTTCTTCGCTTTTATTAAAGTGATTAAGTTTATTCAAAAATGATAATAACTCAAAGCCACTTAGCTTTTCATAGCTAATAACTTCATAATCTTCAGCCTTTTGAATAAATGTCGAATAACACTCATCCAATTCTTCTAATGTTGGAGCATTTAAAATTAAATAAAATGTTTCAATCAAATTGCTTTTAACTTCATTCTTAAAATCAAGTTCATTAGCTTTAAAATAACTATTTCAAAACTCTTTTGTTTCTCCACCGCTATTTAACATCTTTTTACTTTGAATCGTGTTTATATAATTTAAATTACGTCTGTAATCAATTTGTTCATCTAATTTCACTATGCTAAATTTATATTTAAAACTATCCAATATTCTTGCAAAGTTTTCAATCAAAACCACTTTAGTATTTTCATTTTCAGCTCATATATTTTTTCCACCTAATTTAAAAACACTAAAGTAATTACTATGTTCACTCTTTTGAAACAACTTATTTGAAAATGACTTGTTTTTAACAACATCGTTTTCTACTAATGTTGAATATGGATTTAAATCAGAACTATCTCTTACATTCTTGTCATCCACCACATTAGAATACTTCTTTGGCATCGCCTTAAATTTAAACATTCTAATTAATAATTCATATATCTTGCAATTATTCTTATGTGTTGGTAATATCAAAATAAACAATAAAACCAAAAGTACACTAAAAACAATTCATTTATAAGTCTGCTTAACTGGATACAAAGCAACAAATGAAATAAATCAAGATATACAAGTTATTACAAAAAACTCGATAATATCTTTTCAACTAAATCTTCTTGTGGCTTTTGCCTTCTTATATTTTAATCGTTTAGGTTGTAGCACATTTCTCCCTTCTCATTATTCTTTAGGTTTAAGGAATAATCCTTTTCAGCCCATCTTCTTTACATTTTCAGCAAGCTGTGCTCCACCTTGAGCAGCTCTACCCGTTTTTGCAGCACTATTTCCCGCAGCTGAAGCCGATTTAATAGTGTTTTTACTACTTACACTAGTTCCAATAAATGAAGTTATAATTTCGCTTATTTCGCTGTATTTTAACGCTGTAGCACCATAAATAATTATTGAAGCTAAAGCTTGTAATACTCATCCAAAACCATTTGTTGATGATGCAATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAAAACAACCTATGCAAGCCATTTATAAAAGCAACAACAATATCATTCCCTTCGCCAATCATCAATGTAATTATTTTTTGTACAAATAGATTAAAAAGTAAAATAGTAATTTGAAATGCTAAAAGTATAAACAAACTTTTAACACTAAATACCAACGCACTTCTCATTAGTGATACACTCTTTTCGCTTTCACTAAAACTGAACTTTATAATCAGCACAAAAAACATCACCAGTCATATAACCGCCGAAACTATCGCAAATCTAACATACATATATGGGCTTTGATGGTCCAAAATGTTTTGTCTCGGCATTATGCCAAAAATAACAAAGTTTAATAAATCGAAAGTTGCTAGTTTAACAACCGATAATAGAATCGCTAATAATATAAAAACAATTAGCGATACAAAAAATAGCAATAAATACAATACATATGTTGGACCATATTTGAAAATCTCCATCACACGGGTAAAAAATCCACTCATTAGCTAGTCTTTCCCCCGCTTGCTTGAATACTAATAACTAGTTCAAATATTCCTCAACCCACTATAACTAGAATTAATACAATTCCAGCTCACAAAATCTTTTTATTGTTTTGCTTTCTTTTTTGTGGATCATCTTCATGTGAGTTTTGTATCCCCGCCACAATCGAGGTAATAATAACGCTAATAACAGCCACCACAGCCACAAGTCCAAATATGATATGTCCAACTTTCTGTACTTGCTCTGTTAAATTACTTAATCCTTCTGTATCAATAGGTTTCATTTTTAATTTCTCCTTTTTGTCTTAATAAATTAATACAAAAAAACTAATAGAAAAACATCTATTAGAAAAATAGTTCTTTGCACACATTATTATGTAGTAAAGTTAGCATAATTTTTAATCTAAAGTGCTTGTATCCAAGCTTCCATTAACTCTATTATTCCAAATTGCAATTCAGCTATGAAACTTACTTTTAGCTTCTTTTTTATATTCAGCCTCTTGCATTTTATTCATAAATTTAGTGTTTAATTCAAGTTCTTTAAAACTTTTATCTTTAGCTTTAAAAACAGTATTTGTAAAAAAGTTTATGTATTCGTAAATTGGAGCATATGGTGTATTGCCTGACTTTTGATTATCATATTCAGTAAAACTATATGGCACTTCTCAATCATTAGCCCCATACATAGGCATTAACACATCATATAGTGTTCTATACGCCCATACGTAGCTATATTCATAATCAGCAAATTGATTATCCCCAATAGCTACTGGAAACATCTTAACTAAGGTTTTTTTCTCTTTGCCTAATTCTTCCATTTCCACGCCAAAGACTGCTCCGTATTTAAGAACATCAAGCAATGCATTTGTTGCTTTGTCCAAGTACTCTTTAAAATAATTTCTATATACTTGTTCAATTTCAAACCACTTCTTATTTCCAGCTTGAATAGTTCCAAACATTTGCAATAACTCTAAATACTTTTTATAAGCTTCGTTATTAACTAACGTTAGCTTAACCATCTTACCAGCCATCCAAGCAAAACTACTTAAATCATTTCAAAGATTATAATCATCACCCGTAGGCAATTGCATTATGAAATCTTTGTTTTGTTTTGCAGTCTCATAAATCTTAAAGCCTTCATGGTATTTTTCAACATTACTTCTTGTAAAAGCTAAATAAAACCATTTTGAATAATCTTTTCCTAAGTCGAAATAATTATTCCACATTCCTTGTTTATATAAACCATCAATAGCAATATCTTTAAATGTCTTGCTTGTGCCTTTAAGAGCAAAATCATTGATGAATTGTTCATAGTAATTATCAAGCTTCATATCAAAATAATAGTGATAGTGTTTTCTTACTTCCTTTTGGTCCTCAAACACTCATTTATTACCATGATAACTATCCTTATAGGCAAATACATCTTTTTGCGATCCTTCTAAATAACTAGCCATTGAATTAATATAATTCTTTGAATCCACAAGACCTAAATCTTTATATTCAGGATTCAAAAAGTTTCTCATCATATTATTCAACTTCACCGGCGCGCTATAATCAACATCGAATAAATCTTTAATTTCGTTTAATTCAGGTTGGCTAGTATACCAGTCTTTATCCAAGTCTAAACGAACATTGATTTTAGGCATATACTCGTTCTTTTTTCAATTATCATTTCTATACACATCGGCATCATATTTTTGCTCATTATGCAATACTTGCGCAAAATAATATTGATTGTCCTTCTTTAGAAAATCTTTTGGATTCTTTTCTGCATCATAATGTACATAGCCATCCACATCGCTCCAATCCGTGCTTTCTCCGCCACTAGGGTTTGGATTAGGGTTCGGATTTGGATTTGGAGGTTGATGTGGCTCAAAAATAGGACCAAAACAACTCGTCGTTAAACTTGCTAAAGGAACTATCGTTCCAATTGCCATAAACTTCATAAACTTTTTACTTTTTTTCATAATAATACCTTTACATAATAATTTTAATGCAAATCACTATTCTTCTTACTTTGTTTTCAGTATTCTATTCGTTTTTTCACTCAATATACTGGATATATGAAAGTCGCCATTCCAAGAAATAAAATTATTCCTTCGATAACTCCATACTTAGTTTCTGTCAATTCTTTATCAACATACATTCCATTAACGGCCACAAAAGATAAACTTAAAATCATCAGCATTAATATCCAGAATACTATGTAAATAATAAAAAACTTAAATTTCAAATAGTTTTTCATTACAATGTTTGACCGTGTTTTTTAGTTTCATCTTTTACTTCAACATCTTTGTTAAAAGCTTCTTCTTCGTATTTATCATAGACGCTTTGACACAATGGATCCATACCAATATATTGTTTAAAAGGTTCCATCATTTCATCCTTTTTCTTTAGTCTCGCTTTAACAACCTTTTCTTTTTCCTCCTTAGGTAATTTTGTTTCATCTTTATAAATAATATCTTCATCATTACCTTCAGCGTTTCTATCAACTTTTTTAGCACTATAAATAATATTGTCAGCAACAATTTCCATTTTGTATTGCTTATGATTATCTCTATCCACATAAGCATTTCTCTTTATCTTTCCAATAACTTCGACTAAGTCGCCTTTTTTATTGTACTTTTCAAAATTGCTTGCTTGCAAACCAAAAGCTGTAATATTTAGAAAATCAGGTTCCCCACCATATTCATTATTTACAGCAATCACAAACCCTAAAACAGCTTTACCACTTGTTGTTTTAGCTTTAAAGTAGTCTTCACTTACTACTCTACCCGTTAATATAACTTTATTCATCTGCCTCCTTTATTTTGTATTTTTAACTTTTGCTGTTTCACTTTCTTTTTCTACAATACCAGCAACATGTTTTTGAGCTTCTTTAACAGTTTTATCAGTTATTTCAACCTTATTATTTGCATCAATACTTTCTTTATTATTTAAATCAATGATTTCATCTAATTTTAAAGAAGCTAAATTTTCTATATATTCTTTTTTTATGTCATCAATATCTTCTGGATGAAAATTTAAATAATCATCAATATAAACATATTCTGTATCGATTCTACTTAAATCACCAAGTGAATAAATATATTTACTGTTTATTCCATTTTGTTTTAAATCTTCATCTACTAAATCAAATAAATATTCACTTTCTATATAACCATTTTCACCAAAAAAATCATTAAATGAGAATTTTAAATCTAAACTATTATTTTCTATATTTTCTAAATCTTCGACATTGCATATATTTAATAAAGTGATGTATTGCTCCAAATTTTTTTCATTTGTATCTTCAACATAAAACAATTTATCAAAAATCTTTTGATTTATATCATCGTCTACATTAGACATTTTGTCTACTATAGATAATACTTGTTTTTTATATTTATCCCGCATAGTATTATTCTTGATATTTTTTGCTAAATAGTTAAATTTAGCTACAACTTCTTCATCTGAAATATCACTTAATGCATATTGAGTTAGATAATACAAATCCTTAAAACAAAAAACTTTTTCAATCATTCTTTTTCCTTTCTATTTAGCAAAAAGCTTATTGCATATTTTTTTATTGCTTTCTTTTCTAAATTTTTCATATTTTTCTGGTTCCATTTTTTTGAATTCTTCAAGATATTTTTTAGCTAAGGTACTTTTGATACCATTTTGTTTTAAATCTTGATCTATTTTTTGTTTTGATTCTTCACTTAATAAAATTTCATCAAATTTATTGTTTTTAATTTCATGCATAATTTTTCCTTTTCCTTTCTTCTTTTCCATAGTTCCTCCTATTTTTTAGCCTTTGACTTTGGATTTTCAATTTTTGTTTCTTTAGCAATTTCTTTATTCATAGCTGCATTTTGTGCTTTTTCAATATTCGCATTATCATATGCGTCAGCTATAATCTTTCCACTTGCTAAATCCTTTTGGTATTGGCTCATCACAGCCTCTTTAGTTGAAACAACAATTTCTTTCATTGCTTCATTAAACGTTTTTGTTATCTCTTTAGCAACACCATCGCTCAACTTAAAGTTCTTTGCAATTTCCGGCTTTATCTCATTTTTAGCTAAAGCTTTTTCTTTTCTTTTATCAAAGAAATATTTAATAGCAGTTATGCCTATTACTAAAGGCAAACCGCCCACTAAATATGCTTGAAGACATAAGAACCCTTTTAATGCAAGAGCAGGTAATTTCTTCAAAACTTCCTTAAAGTTCTTGTTTCTTATTTCCGCTTTCTTTTCTTTATCAGCTTCTTTATATTCTTTTACCTCTTTTTCTTTTACTTCATTATTAGTAATAAAATTAGTTTTTCCTTCTACTTCTCCTTTAGCATCATATACAACTTCTTCGCCAGTTTTAAATCCATTTTCATACATTGTGCTTTTAACTAGCGCGCCGTCTTTATATTCTTCAACAGCCCCATTTAATAAACCATCTTCAAAATGTGCTTTATAAAGAATATCGCCCTTAGTGTTATAAATTGTTGCTTCACCATTTAATTCATCATTTTGGTAATTAGCAACGCCAACGAGCTGATTATTTTCAGCAATTTTGTAATAGCGTCCACTAAAAGCTCCTTCGTTCATTTCATAGTATTCTTGACTATTATCATTTAAAACATTATGTGCTAAAGTCAAACCACTTTTTGTACTAATTAATTCAAAATTAGTTCCGTTCTTTTCATAGTTTCTACGATTAATAATTTCTTCGTTTTCTAATGCATTAAATACTTGCATATTAAACCTCCTTAAAATAATGTTTAATCTTTTCTACTCTTTCAACTTGCACCCAACCATAATCTCCTTTTTCTATAATTTCATTGGCTATCTTGTCTGCCTCATTAAAGTTAGAGCAAACTGCATATGATTTTGTTTTCTTAGATTCAAGATTCTTTTTGACTCTAATTCTGTAAAAATATCTTTTCATTCGCCTTCTTCTATGTTTTAGCTCCAGCTGTTTTAATATCATTCATCTGGTCTAAACATATATTTATCGACTTCGTGAAATCGTTCACTAGTTCACTACTTAATTTGTTTTTGTATTTTTGTTGGAAACTTAATATCTTTTGTCAAGCAAGTGCTAATTTAGGCCATTCGCTTATCTTGTCTTTATACTCGCTAAGCTCCTTAATACTTCCTCTTGCATTTTGCATTTCCAAAATAAGTTTCTTTTGAACTTTCTTATCATTAAGTTCTTTATTGAGGCTTTCCTCTCTAGCTTTCTTGGCTCTTGAACTATCATTCAATTGGTCATAGTTTTTAATAGGATTTCTAACCGGTAATTCAACATCTGGCAAGTCTTGATAATTTCAAGATTCATTCACAGTGTTTTCTGTTTCGTCACATTCAAAAACATAATTATCATTACTAAAATATTTGTAAGCTAATTTTGTTTTTACTATCATAGGAAGAAAACCAATTTTTTGAATAATGGCATATTCTTTATTCTTCGCTTTAATTTCCGCCACACTCATTAAAGGCTTTTCTTCCACCGATTCACTTTCTGAAGATGAACCACCATTAGCTGATGTTGAAGATGATTTTGAAGTTGATTTTTTAATAACTTCTTTTTTGCCTAAAGAATTAGATAAATCGCTTAAAGTTTTTTCATCAATAGAGTTCAAGAAATAAGTAAATTGCAAATTACTACGAATAATGCTATCCACATTATGATTAGTGCTTGTATTGTATTTTTTAAGCTGATTGTAATCTTGCAATACAAGTTGGAAAAATACATTTCTACTACGCGATATTGTGACCTTATTATCTAGATTCAATATTGGAGGCAGGTTCCCCGCTTCATCTAACATGAAGAAGAACTTCCGTTCAAGTTTCAAGTCTTCCTTACTATTAGCGCAATCAATTAAAGCTTGATATATTTGGTCAATAAGCATACTTACTAAGAAGTGATTACTTGGCTTATGGTCTGGATAGTGAATGAAGATGACATAAGGTTCATTCGAAGATACAATACCATGTAAATCAAAACTCTTTGTTTCTCTTGTTAATCTCTTTATAAACTCATCTTCAGTAAAGTTTTTTAAAGCACTCGTAGTATGTGATAAATTACCCGTTAATGTTTCAGGAACAATATTTGCAATTGTCTTAACATTTTTAAATAATTCAGCTCAATAATACCTTTTATTTGTTTCTGCATTTATATAGCAAATTGAGATTCATTTTTGTTTAGGATTTCAAGTGTCCAAATTACACATTGAAGCTACCGATTGCAAATTGAAATGCCTTTCTGGTAATAAATCCGGCCTATCAATTGAAAGCAACAATAGAAATTGAGCCACAACATTAACAATCAATTTAGCATTATCAACCCACATAGGATTACCATCGCTATTATTCCCTCATTTTAGCTGTTCAACAACATTATTAATTTCGCTTATAGCTTCATTCATGTTGTCCTCTCATTTAGGATGCCCTTCCGGCACTCTATGAGCTAGTTTATAAGCAAATGATAATGGATTCCAACCAACACTTTTCTTTGTATCGCTAAAATCTACCGCATATATTTTGTATCCTTGCTTTTCAAGCTTTTTACCAACCATTTTGATAATTTCTTTTTTTGGATCCGGAATACAGAAATTAGCTCTATATTTATATCTTAGATGTGAATTATAAATAATATTAGGCAATAGTACACGTTGTGTTTTACCTGAACCTACACCCCCCAAAACAACTGAGTTAATATCACTTTCAGGTTCATTCACTCATCATTTATTTAGATTTGAATCGAACTTAATTACCCAATTAGCGTTGTTTTGATTTCCCACATAATTTGGTGCAACTTTAAACTTCTTATCGAAAGTTTCATTACTTACACCGCTATTATCAATTTCATTTCTATATCAATACTTATTAGGTTTTAATAATGATGTTTTAAGTTTATTTTTATAGAATAAATAACTTATACCCGCTATCGATGCAATTAATAAAAATGCGTATGAAATTAAAAACTGTTTTGGAAATGCATAAATGCTTGCTTTTGTCTGTGCAAAAAAGTCTTTAATTGAAACCATCTTAAAACTTTTCAAACGCTTAACATTAAATATAATGGCTAGAGCTAGGAAGATGATAATTGCTGCAAGAAGTATTGCAACAGCACTTAAAGATATTTTTAATATTTTCTTTTTCTTATCTCCCATTATTGACCTCCGCCTTCAGCCATAAAAATATCAAGCTTTTGCACAATAATTCTTTTAGCTAACTTTTGCATTGGAATTGCTTTAGCTATGCAAAGGCATTCTTCAATTATTTGACAATAGCCTTCTACGCTTAAGCCTAAATGTTCAATAAACTTCTGGTCGCATTTATATTTCATTCTTATAGGCTTTATTAATAACATCATGCAAAGGAATACAACCATTAATAATGAAGACGACACCGTAATAGAAATATTTCAAGCAAAACTTATATTTAATACATTCACTAGGCTAGCAAATATGAAGAAAACTAAAATTGATATTGAAAACAATACAAGTGCTACGTTTCTTAATGGCATATTGTTTTTCAAAATCTTTTTATAATGAAGTAAATTGATACCCGTTATAACATCATTCCAATCTTCCGTTTCTATAATTTTTTTAACTATCTTAGATATATTGTTTCCCATAATAAAATCCTATATTTTGTAATAAGTCATAATGTTTTCGTATTCTTGAATGCTTTGAGCTTCAAAAATCTTCTTAGCTCTATTAATCAATCTTCAAGAATCATCATTTACATTCAAGTTATAAAAGAATAATTTTGTAAAGCTTTTAGCACTCTTAGCATTTCTTGATTTTAGAATTGCTTTTATTATCTTGTTCCCTAGCTGTCTAGAAAACTCATCTTTTTCAGTTCTCAAAACATTATTGATTCTCTTTTTTAAAAACGTTGAATTCGTTTCATCCTTTAGTTGCATAACTAAATCAAATGCCTTTTGATAGTTATCCATCTTTCCTTTAAAATCATCATCATATTTATATAAAAATGTTTCTATCTTGTTTATCGCTTCTTTAACTTCTGGTGAAGCAAGTTTATAAGACTTATTGAACATATTCTCCATTTCAGCTCTTACATAACAGCAAGCCTCAATAAAGTCTTTGTAATCTTGGCTTTGAACATTCTCAAGCGTTCGTTCTGTCGAAGATAAAACATCTTTTCTGGCATCTCATAACTTACTTGTTAAATCCTTAAGATTTTGATAATCAAAGTTTTTAATAAGCTTGTTTGTAATCTTCATTCTTAGCATTTTTATATCGTCGATGTTTAATTTTCTTTTTGCTCTTTTTTTAGTCTTTTCAAAAAACATAAAATGGATGTGTGGATTATCTGTATTACCATGTATTGAATAATAAGAACTAAGGTTATCTAAATTAAAACCGCTAATGCTATAGAAGTTTCTAAACTCCTCTTTAACTAACTCAGCTCAATCATTAGCAGTTAAAACATTATGTTCTAAACCTAAATTACCTAAGTTTAAAATGCCTTCATAAAATGTTTGTTCATCAATACTCTTTAATTCTTCCTTAACGTTATCGATATTTACATCAGCACTAGAATCGTTTAGCATATCATAAACACCAGAATATTGGCTACCCATTTTAGACTTAAGATGATTTCTATATCAGTTAATCTTTTCTTCTCTACTTACTTCCTCTAAAAGCATTCTATCTTTTTCGCTATCATCAAAAGGACTAAATGCTACACACTTATCTCCTCTTGCAAAATAATCAATAACTGTCCCACTTGATACTCAACTACTTAAATCATGACAATAGCCTAAACCAGTTTTATAGTTAGTCATTTTAGATACATAGTTGAACTTCATATAAACATCCATTCTCCCTCCTCTTTAACTAACTAGCTCCCTTTAGCTATGCAATGAAACTCAGGAGTAAGTTTTGGTATATTCATAATTTGAGAAAGTCTTTTAGCTAAGTTTTTACGATTTTGTAATCCCAAATAGAGAAAATCATCTCCAACTGATGAAGATAAAAACTTTTGAGCAAACTTATAGTTAACTAAAATTAATTGTCCTTCTTTGTATTTCATTTCTTTCTCCTTCGTATTAATTCATTACATTCATTAATACTCCTAATTATTTATCGACCTAATTTGGACATCATCAGCATACTTATTCTAAAATGTCTTTAAACCCCAAAAAGGTTAAATATGAAAGATAACACACGAGTGTGCGTCCATAAGATTTTCTAAAGCAAGCAGTAATGAATAAACCGCAGTAATAATAATTGAAGCTTATTCATTACTACTCCTCATTTGCTTTATATGCGTATTACAAGATACCTTTGAGTCTTAAAGCTTAAATGCTGCAAGTTATCGACGCTTCTTTGAATAATAAATAACTCATCAGTTCTCAAACCTAAAACCAAACATATATTTTTCAAGTATCTGTAAGCCGTAGAACCTTTTAGCCCTAAAAAGTTATTTTGTACAATGACTTTCATCTTTCTACTTTTAACAAACTTTTTAGTTCCAAGTTCTTTTGATTTTCATACAAGCGTTTTATAAGCTCATAAATTAGCTAATCCAAACAACAAGAAAATCTTCCAGTCTTTATAACCACTAATAACAATGAACTTTCTATCGTCATTAGTTAGCTTAAACTTCTTAATTTTCACAGCGTTTCTAACTTTTAAAATTAGCCCTATATCTGTTAAAAACTTTACCGCTCTTTGCAAACTTGTTTTCTTGAAGCCAAAAGCCACTAATTCATAAAAATTTACATAGTGTTTTTTAGCCCCAAGAAACCTTAAACAGACATACACTTCAAAAGCAACTTTTTCAGTTCTCAAAATCTTATAGTAGCTTTCACCGAGTACTATCTTTGTTTTTAAAATTTCACTAACTGTATCTTGGCTTAACTTGTCATAAACCTTCTTGCCAAAATAATTTTTTCTCCATTCCAAGTTAGCTTCAAACTTTCTAACTTCGTTTTCGCTTCCTTCATTTGCAATTAACCTTAGTTCGCCGTATACACTCATAATTTCTCCTTTTTTTTGAACATATAACAGCGACTGTGGTATAATATATACAACAGGTCGAACGATTGGTGGTGTTCTAGGCGCTGTTATTTTTTTATACTTTTGATCATTTTTTTGAAAGGTATATTAATTTAGCAGAATACGAAACTAAATAAAAATATGAATTAATGTTTATGAGTACTTTTATAAATAATCAAAAGTGAAATAAAAAAAGTGTTCAACACACTTCTTTCTATCACTTCCAAATAAATTTATTATTAGGCAGAAATGGTCAAGGAAAAACTCTTCTACTTAATATTGCTTCAATGCAAGACGATAATTTTACTGGAGAAATATTTTACGAAAACGAAAACATAAAAAATTGTAAAGAATGAAAAAATATTTTTTTCTTACCAACAGATTTTAATTTTCCAGTTTTTTTAACTTCTTATAAGTTTATAAAAATTTATTTAAAGCTTTTTTCAACAAAAATTTTTGGCAAATTTGAAATTGAAAATAAATTTAAAGAATTCGGTTTATTTGAATGCATAAATAAAAACCCAAATAAATTGTCTAGTGGGCAAAAAAGAACATTATTGTTTTTACTAATCAATATGATCGAACCTAAGGTTTTACTTTTAGACGAACCTGAAGCAAATTTGGATCCTTATGCAAGAAATCAACTTTATCAAAGACTTCAAGCTTTAAAGCAAAAGGGCGTGTCAATAATTTTAGCATCTCACGTTATAGATTCAAATAATGAAATAATTGATTACATTACTATTTTGAACGAAAAGGAAATAGTATTTGAATCAAAAATAAACTCAGTAAAAGAATCATCTAGCATTTATGAAAATTTTAATAAAGAAATATATGATAAGATTTAAAATAAATGACAAAAAATATGATTTCTTAACATACAACGACATTTTAACTTTTTTTAAACATTTTGTTTTACAAAATATAAAAATAAATTAGAAAGGATTTCAAATGAAAAAAATTATTAAAATAATAAGAAATTTAAGTATTTTATCACCAATACCAATGGTTTTGAATTCATGTTCAAATACCGATAAGCCTAAAAAACAAGAGGAACAAGAGGAAAAAAAAGTAAAAGAATATCATGTTCCGACTTCTTGCGTAGATTATGACAAAGATGAATTAAAAAATATATTAGAAAAAAATACTAAATTAGTAAAAAATGTTAATACAAATAAAAAAGAAAAATATATTGATTATGATTTCGTTTATGACGTTATACAAAAATGATATATTGAAAAAAATGGAAATCAATTAACAGATGTTTTTTATTTTCCTGCTTTTAATGCAATATCAATACATCCAGAATATACTAATGATAATGTTAAATTATTTTCTAGTTATTTATTTTCATCTAAAGATGATTTGATAAGTGAATTAAATTGAATAAACAACAATGAAGAAGAAGCTAAAATAAAATATTCAAATAGAAATTTTAAATTAAGTGAAGACGAAAATTTAAAAAAAATTAAATCAGAAATAGAAAAAGCTATCGAATTAAGAAAAAACAAAAAAGATATAAAAAAATTCAAAAAAATAGTAATGCCGTCAAACTTTTTAACTATGAATTCAAATGATAATTTTGATTGAAAAAGAGAATTTTCTAGAATTTCTTTGTTAAATAATTGCGAATTTGTATTTGATTCCAAATGTTCTAGTATTTTTTTTCATGATAGAAATTTTGGTAGTTGAGAGCTTAAGGGAAGAATTAATTTAAGTGAATTTAATAATGTTAAATTAAATTATTTAGACAATTCTAAAGATGGTTTGTCTTGGAATAAAAACATCTATTTTTATTTAAATAATTTTATAGACGAAGAAAAAGATATTATTGTTGATAAAAATATATTTAATATGGAAAGTTATGAACAATTTAATAATGTAAAAATAAGAAATCTATATTTAAGAAATTTTAAATATAGTTTTAATACAGGTGGTTTAAATTTAGATTATTTAAATATTGCAAAAAAATTAAAAGTAAAAAAACTTATAATTGATAAGGATTTATCATTTAAAACATCTTTCAAATTATCCTTAAAAAACACAGATTTCGAGGACATAGAAGGTGCAGAATATTTAATTAAAAACTTATATGTTCCGGAATTAAGTGGAGGAAACTCTGATTTATTTATAAAATTATTGAAAAATAATAAAATTCCTAAATCTTTCAAGATTAAATTAAAAGATTATTCGTTTCCACCAGAAGAAAATGATTTATACGTTAATTGTCTTTATCGTTTATTTAATTTAAATTTAGATTTAGTAGATTTATCCGATTCTGATCCTGTAAATATTTTATATTTTGGAAACCCTAAAAATAGTCAAACCGATAATGATGCATTTAACTGTTATACAGATAAAGTAATTTATCCTAAAAAAGTTGAAAAGGTTGAGGGGGCAATAGCTGCGCATAGATTTAAAAATATTAAATTTTGAAAAAATGTTGGATTTAATGCAGACAATTTTATTTATTTCTTTGACGAGCGAATGAATACAGATGATTGCAAAACACTTGAATTTGAAGAATTTGAAAATGTCTCAAAACCATTTGAAATGTTAAACAACGCGTTTGTTCTTTGACCTAATGGTAACAAATGAAATAAACCTTATGTATTGACTATAAAAATAAATAATTTAACAGCTTCAACGTTAAATCCAATTTATAATATCACACTCGGCAAGGAATATTTTGATGCAAGTCATTACAAAGATATTCTGGACGTTAACACATTAAATTTTACATTTGCTGAAAAAAGTTTAATGGGCTTTAAAACTGTTAGATGCTTAAATGATAATTTTGATGTATTTAAATCGTTAAATATGACAAAAGTAAATCACGAAGGAAAAGAAAAGTGATTTTTCAAAAATCATGCTGATTACTTTAATGTTCCAGTTTATGAATACAGAAGAATGAAATATAACGAATTAAGCTTTACATTTGAATGTAAAGATGGAACTTATTTGTTGAAACCAGATGAAGATCCTTATTTTGCAAAAAATGGTGAATTTTCATCAAGAAATTATTTAACTTACAATATTCAAAAAATTAAATAATGGGACTGTAAAAAATTTTGTGTAAAGTTCTAAAATCCTTTCTCAATAAATAAAATATTATCAAGAAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAAATTTGATAATTTTATGGGTTATGAAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGATGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATATTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGCATTAATGGTGATGGACAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCACAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGGAACTGATCTGAAATTTCTACAATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTAATTTTGAAAATGAAGAATAATTTTTTCAAATTTTAAAAGCTTTGCTTCTTTGGCTTCAAAGAAGGCCTCTCCCTTAAATGAGTTAAGGGAGACGGGAGAGGGATTAAGATTTCTTGCATGGCAAGAACTTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTGCAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTTTATTGAAAAAAGGAGAGACTTTACACAAAATTCTAAACAGTCTCAATTAACCTCTATTTATTTTTATTTCTTCATTTAAAACTATAACTACTTGCTTTTTTAACTCTAAAATCACGATAAATTGATTTGTCTTTTTTAGCTTGATTGATGTAATAATCAATTACATCTGGATTAAAGAAATACTCACCATTATTATGTTTATTAACTATTTCCTCTAAATTATCGTAAATCATATTTATCAGTTTTTTAGGATATTTGAGGCGTCTAGAATTGTAACGAAATTCTTCTTTATCTACAATTGTAAAATCTTTTTTAGGATAGCTTTTAACATCTAAATCAAAATCAACAAATTTGATTGTATTATCTTCATAATATGGTTTTGAAGCTAAATTTACATAGACATAATTACTTTTTGGTTTTAATAAAATCAAGGCATTATACATACTATTTTTAGGCATAAATCATAAAACATATTCGCGATATACTCAACCTTTTTTATCAGTTTCAGCTACTTTAGTTTTATACATAAATAGAACATAATGTTGCGGTGTATTTCTTAAAACCTTAACACCGTTTCATTGGCGGTATAATTCACCATTAAATTTAAAAGCTTGGACATTAATAATTGATCCAATTAAGGGAAAAACTATTTTTTTAGGTTTTAAATTTTCTTCTTTAATAAGAAGATTATCCTTATTAACTAAAGACATTCATTTCCCTCCTTTTAAAATATATTCCATATTTTTTATTTGATTGTTTTTTGCTTATAAAGCTAGCTTCTATTTTATATGAATTCGTTGAATCTAATTCACTAGGTGCATCACTACTAGTTAAATATCTATCTGGCAATGGATCTTTTTCATATTCTTTGATTAATTCATTATCTTGCATTGCACCATCATTATTTAGATCTTGTCAGAATTCAATAGGTGAATTTTCTTTTATATTTCTATAATCAAAATAATCACTTGTATAAGCTGCTTGTTTTGAAATTAAATCACTTAAAACCATATTTGGCGATAAGGTAAAAGTAGTATCTAATAAATTATCTTTTCCCTCAAATTTAAATGCATTTAAATAATTTATTTTAACAAACTCTTTTTCATCTTTATTATTTGTGAAATAAAATCCTTTGATATTTTTGTTATCTAAATAACCACTAATTCTTGTTTGAAAAATATTATTTTTATCAATTTCAACTGAATAACTATCAGTGTCTCCAGCAATTAATTTTTTAACATCTATTTTGCTTTTAACTTGAGCTATAGTTTTACCATAACCCATTGTTTCAAGAAACAATTTGTAAAATAGTTGTTGATTTTCACTTTTATAAATATTGTTACTATAAAAGTTTCGATCAGCTTCTAATTGAGCTTTATGCATTGTTCTTGATCAATCAATTGAATAACTATTGGCATAGGTCAACAAATTATTTGAATCTTTAAAAGTAACACCATAAAAAGATTCAATTATATCTAAATCATCTATAGTATCTGATTTAATTATTTTTTCATATAAATAATTATAAGGATTTGATGGATCATTAAATGGTATGTAAGCATATTTTTGTCATTCTCTTGGCACTAGTTCAACTAGAGAATAATAATAAGGCAAACTATTTATTGAATAATCGGCTATTAAATATTTTGAATCGAAATCTTTAGTTTCTCTAAAAGTTACTTTGTCATTTTCATCTTTAATAGATTTCAATTTATTTAAAGCTTCTTTGAATTTTTTATTATCGTTTTCATTTGATAAACTTCACAAGTCATTAAGACTTAGTTTATAAGGAACAAATGAATTGTCTCCTGGAAACCATGGATTTATTGAGTTTATTCATGTATCATCATAATTTAATAATTCCTTAAATTGATTAGCAAATTTTTTATATCAAAAATCACTTTTTACTGAAAATTCTAAATTTGAACGATAAGGAATTGTAATTGCTTTATTATTTTTAACATCTTCAATTGAACCATATGAAGCATAACTGTGTGCTCAATGGTGCATGTATTCATGAAAAAGTGAATAAAGTATTTGTTTTACTTTGACACTTAATTTTGCTCCTTTTTCACTTAGTGCCGCACCATTTAAATAAATTCTTTGAATTGTTGGCAAATAAATACCTGCAGATTCTTTAGTGATAATACTAAAGTCATTAACGTTTATAGAATCTAAAAATTTTATTTCCGGTCCATAAGTCATTCTTGAGTAGAAATAATTAGCTAATAAAGCTAAACCATAAGGGCCTAAAAGGAAGCGTGGTTTTTTGTTTTTATCAACGCCATAAGATTTGTCAATAAATTTGACATTGTTAATAGGATCAATATATACTCAACTGTTATTTTCAAATTTAAACGTTCCATTTTTAACTCAATTTCTCTTATCATATTTATAGCTATGTTTTTTCGTATCATACTTTAAAACAGTGTTATTAGCCAAAATATAATCATTAAATTTTAAATCAATAAATTGATTTTTTAAATCATTTGTAACATTGTCAAAATTATTTAATGAAGAAGGACTTTTTGCATATTGTTCAATATATTTATCTTGATTTATAAGTCCTATTTTATCTTCTTTACTTTGTATTTTTATTTTAACTAAATTATTATTTTTATTTATTGTTTCATTTTTATTATTTAAAAGTAAATAAACAGCAACAAACAATGAAACAAAACACAAAAAGATTGACAATGATATTAATGTTCAAAATAATGCATTGTTGCGCTTTTTTGATCCTGATCGCTTAATATTTTGAGGTGGGATCATGATAACCTTTCATAAAAAACTTACATATTAGTATATTTAACTAACATTTTTATTATAAATGATTAAATACCATATTTTTATAATTTTTGAAAATAATATTCAACAATTTAACCAAAAGTAATAACAATTCCATAAATAAGTGTTAAATTTTGCACAAACTAATTTTTGGTACTTCATACTTGATATAATAAAAATAGGTGGTTACAATAAACCACAAAAGGAGATTTATGTATAAATTTAAAGCAAAATTAGTTTCAACTCAAGAAGTTGTTGCACAAGCAAACTCACTTGAAGAAATCGAAGGATTAATCCTTGGCTTCCGTCGTAAACAAAAATATGACGAACACACAAGAGCCAACGAAAAAATCCAAATTATACATGTTGAAAGGGATTCTTTAAAAGGTAAACATAAATCAAAAGAAGAAATTCTTAAAGTTGTTTAATTACAATTAAATATAGGGGCTAAAGCTCCTTTTTTATGTGTCATTTTTTTCATAAAAGCACTTTTGAAGTCTTATTTTAATATAATTGTAATGTTAATATTGAGCTTGGTTGCTTATTTTTTTAGCCTTTTTTCAATATTAATTAAAAGAATAAAAAAATAGAGGAGAACTAATGAAGAAAAGATTTTTATTAGGTGCTTTAGCTCCAGTTGTTGCTTTAAGCCCTATTGCTATTGCAGTTAGTTGTGGAAAACAACCAACCATTGAAGAACAATTCGCTAAATTAAATAATGAAGGAAAATTAGCCTTTATTAATGCCATTGACTACAAAGGAATTATGTCAATGGATGAAAAAGAAAAATTAATTTCTAAATTAAATAAAGATGCTGCTCAATTTGGATCAATTGTTTGATACATTAAATCAGTTGAAGCAAGAATTGCAAAAGAAGCTGAATATTCTAGAGCAAAAGCTGTTTTTGACAACTTAATGAAATATGCTGTAACCGATGGTTTTGAATATGGTCAAAACTTTAATACAACTAAACAAGTAAATAATGCTGCAACAGATAAATACATTCCAGTTATCTTTATGGACATTGATGAAACTGTTTTACAAAATGACTACACAGAAACATTTGGTATGTTAAATGGTGGTTACAATGGAGACACGAAAGAAAAGAACGACCTTAAAGGAAATAGATTTGCTGTGCCAGGCGCTATTGATTTCATTAACTATGTACAATCAAAAGGTGCTTTAGTTGTTTATAACTCAGATATGAACCAATCTACAGCAGTTAGAGAAGCTGTTAAAAAGAATTTGATAAACTTAGGTGTTAAATATGTTTCAGACTTCCAATTCTGAATGCGTGGTTCAATGCCTTATGTAGCAATGGATGGAACTAAAGTTATTACAGACGAAATGACAAAAGATATGTCTAAGGGTGATCTTAAAAGCCTAGCTGAAAGAATGAAATTTACAAAAACTTTTGAAACTACACCATGAAGAACATGAACAAATTCAAATGCAGCTTATGTATTAGGCAAAAAAGTTTACAAAACAGACCGTATGAATGGTTTAGACGACAATGCAACAGGATGAAACTTAAAAACTGTTGATTCTAAATCAGGTGATGCTGTTAGATTGAGAACACTTATGAGAATAGGCGACAACTTTAATGACTTCTTTGATAGAAACTCAAAAGGTAAAGACAATGACAAAAGAACTCAACAATACGTTAAAAACACAGACATGAGAAAATTATTCACAGATTATTAATGGTGCTCCAGGATTAAGATTTGTTAAAAAAACAGATGGTAAATATGGAGAATTTCAAAAAACAACATACTGACAAGGTTACAGTATGGTTCCAGGAAATAGTGAATATGGTGGATGATTAGATTCATTCGGATACAGTGAAACTTACAAAAACATCTATGATGAATTGAAGAAAATATTAGCTAATCCTAAATATCAAGCTGAACGTACAATTGCAGATATTTACAACGAAAACTAATAAATCAATATTAATATCAAGCTTATTATTGAGCTTGATAGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAAAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCAACTTTTTTGGGTTATTTTGCTTTAGATGCAATTGAAAAAATAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAAATGTGGACTGTCAAATTAACAATTGAAAAAATAAAGAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAAGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCCGCTAGAAGACTGGTTGGTGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAATGAAAGAAATGAATTTGGCCATTGGGAAATAGACCTTATTGTAGGTAAGAGCAAAGGAACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATGCGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAAAAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGACTAATGCCCTCACTTTTATTTGTCCTGACGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAAATTACAATCTAGGTATCAAGCTTATTATTGAGCTTGATATTTTTTACTTTGCAAAAATAAAAGCATCCTATTGAAAGGATGCTTGGTCAATTACTTGAACTGGTACGTCCTAGAGGACTCGAACCTCCGACCCAATGGTTAAAAGCCATTTGCTCTACCGCCTGAGCTAAGGACGCATATTTGATGCCTAACGAAGTAGACAACTGGTACCCAGAACTGGACTTGAACCAGCACGAACTAACGTTCGAGGGATTTTAAGTCCCTTGCGTCTACCTATTCCGCCACCTGGGCATTTGCTAAAATGCTCTTTAATTATAAAGCAAAATAAAATAAAAACAAATATTTTTTAAAAATAAAATTTTGTGTTTTCATCTATATTTTTTGCTTTTAAAATTATAAAAAAGGAGTAAATTATGTTTATAAGTTATATTCTTAATAAAAGAGGTTTAAAATGAAAAAAAATAAAATTTTTAATAGTTTATTATTAACTCCATTAATTATAACTCCTATAACATCTTGTGGTAGTAAGCCAGAAGAAAAAAAGTCATTCCCATTACCCAGATCTTGCACAGATTATGACAAAAAAGAATTGAATAAAATAGTTGAAAAAAATACAAAATGAATTGAAAACTCTAAAACACATAAATTAGTAAAATATATTGATTATGATTTTTTCTATGATGTTGTAAAAAAATGATATTGTGATGATAATAACAATTTAAAATCTGATAATTTTTATTTTCCAGTACTAGATAAATTGTCAGTTTTACAAAAAGAAGATGACATTGCTTTAGTTTCAAGTTATATATTTTCAACAAAAAAAGATCTTCAAGAAGAACTAGATTGAATTAACAATAATGAAAAAAAGGCAATTCAAAAATATTCTAAAAGAAATGAGACACTAAGTGATGAAGAAAACTTGAAAAAAGTCAAAAAAGAAATAGAAAATGAAATTAAAATAAGAGAAAATAAAAGTGATGTTATTTCTTTTAAAAATTTTTGTATTTCTTCAAATTTCTTTTCTTTAGAAACTAATGATTTGCAAAATCCTAAATTTATGAAAAGCATGTTAAGACTTTCTTTGTTGAAAGACTGCAATGTCAACTTTGACTACAGCACTCGAGATACATTCTATTTCTATAAAAGAATTAATATAAAATTATCAGAATTTAATAATGTTTTAATTAGCTCATTCAACAATTCAAAATATTCTAAAAATTACATGTCAGGGATTTATTTCTATTTAAATAATTTTGTAGCAGAGAAACAAAATATTATTGTTGAAAAAAATAATTTTAATTATCGTGAAGCAAATCAATTTCAAAATGTAAAAATAAATAATTTAACATTAAATAATTTTAAACCAGACTTTGAAAACGAATACATTATAGCCAAAAAACTTGAAGTTAATACTATAGAATTTGGTGATGACTTTCTGAATAATGTTCATATAGAAGAAACGTTTAAAAATATAGATTTTAATGACATAAAAGGCGTTCAAAATGTATTAAAAAATTTAGATTGTTCGAAATCGAAAAATAAAGTTTTTCTATTCCAAAAATTAAATAAAATAAATAGAATGCCAGATTCATTTAAAATTAATTTAAGAGAAGAAACATTATCTGATTTTAGTACTTTATGGATGGGCAATTTAGATACCATTTTAGGCGCATGTAAAGATACTCTTGACTTATCAGAATCAAGTTTAATAAAATGTTTATCTTTTGATGATTATGGTATCTGAGGGCAATCATTTTTCAAAACTAAAAAGATTATCTTTCCTAAAAAAATAAAAGAAATTGAAGGACAAATTTACCCTGGTTCTTTTGTAAATATTAAATTTTGAGAAAATATCGGTTTTACATCAGACAATTTTAAATTATTTTTTGGCAGAAATTGACGATTAGATGAAGACAAACAAATTGAAATCTATAGCTTTGATAGTTCCAAAAAATTTGAAGTTTTAAATGACGCAGGTATTTTCTTAAAAGATAATGGCGAATGAGAAAACAAAAAAATTAGACTTACTATAAAAATAGATTATATGGAATCAAAAACATTAAATCCTATTTACAATATTACAAAAAATATGGACTTGTTTAGAGCTGAAAATTATATGGACACTTTAGATATTAATACATTTAATTTTGAATTAACAGAGAAAAGTTTGATGGGATTTAAAACAATCAGATGTTTAAAAGATGATTTTGAAATTTTCAAAGAAAAAAATATGGAAAAAACAACAAATAATAATGGAGAAGAAGTTTGATATTTTAAAAATCATGCAGATTATTTCAATGTTCCAGTTAATACTTATCCAAATATGGAATATAATAAATTAAGTTTTATTTTTGAATGCAATAATGGAACATACACATTAATTCCCGACAAAGTACCTTACTTTTACGATGGTAATTATTTAACATATAATATAACAAAAATAAAATAGCATAAAGCTATTCCTCATTTTTAAGAGTATCTTATTGATATTCTTTTTTTATTTCAATTCTTCAAATAAATTTGCGCATTTATTTAAACCATAAAAACGCATTGCATCTAAGCCATCTTCTAAGTTTAAGTTCAATAGGTAATCAGAAATGTTTTTATTCATATCAACATTTAAATTTAATTTTGCTAATGCTTTACATAAAAAAGCTTGATCTTTTTCATCTAATAATTTTTCTTTAGTTTTGCCTTTAATTTGATCAATATTTTGATACATGTTTTCAATTGAATCAAAAGTCTGAATTAATTTAATTGCACCTTTGTGTCCAATGCCATTAATTCCTCTTAAATTGTCAGAAGAATCTCCTGCTAAACCTTTATAATCAACTACTTGACTTGGTTTAATACCGTGAATAGTTTCAAAATTGTTAATGTCTTCTAATTCATAAGAAACTTTGAAATTTTCTTTAACTACTTTAATTACACTAGTATTAAGATTAACTAATTGTAAAAGGTCTTTGTCTTTAGAAAAAATTAAATTATTGCAATCTTGATTTTGAGCTAAAGTTGCAATTAAATCATCCGCTTCATCACCAACTTGTTCAAATCATTTGATATTTAATTTTGTCAAAATTTCTTTAATTAAGCTAAATTGTTCAAAAATTATTTCTGGTGCTTGGGTTCTACCTGCTTTATAATCTTTGAATATTTTATGTCTTCTAGTTTCTCCATAAGCATCAAAAGCAATAAATAAATATTTAGGTTTAAAGTATGCTATTAGTTTACTTAAAGTCATAAGAAAAACATGAACACCATTAGTTGTTAAACCTTGTGGTGAACGCATAATATTTTCTAATCTATCCGGATTATATGAAGCATAGAAAGAAGAAAATAATAAATAATTCCCATCTATTAATAAAAAGTTGTCTTTGTTCATTATTGAATTCCTTTTCAGCTAATTAAATTATGTTTGTTGCGACTGAAACTAATTGTAGCTTCAATTATTTGTCCTTTTTTAAGAGGAAGTGAGCTTGCCAATACTTTGTTGTTAACAAAGAAAGTAATTTTAGCTGTACTATCTCTTAATTCTAAAACGCTAAATTCTTTACCTCTTGGTCTTCTTAAATCAGCTACTTCAGCAGCAACAGAAACAGGAATTGAAGAGGTTATTGTATTTAATTTATTTTTGTATTTTGCTTCATAAATTGAAGTACTAAAAGCATTGTATAATTCACCTAAAAGAGCTATTTCATAGTCCATTTCGTAATTTATGTCACGTTCATTTTCTTGATCAAAAACTATATCATAGTAACCCATTTTTTGAATATCTTTTAATGCTTCTTTATAAGTTGAATGTGAAGATAAAACCTTAAAAATGTCTTTAATTTGATTATCACAAGATAGTATATATTTCATGTGTCCAAAGTCTCTAAAAGCATTGGCTTTAATTAATATTTCCATAGCAGCATCTTTAATACCTGCAAAACGAAGTCTAAGCATTGTATCAATTAAATTCTTTTGAAATGGTCCATTTTCATTTAAATCAGCCACAATTTTATTAACACCTTCAGCTCCAAAACCTTTAATCATTTCAAAAGGTAAATAAATGCCATCGTTTGTTACTATACATTTATTACTTGAATGCAAAATATTTGGCGAATAAACTGGAAAATTAAGTTTCTTAATTTCAGCTACATATTTATTAATTGTAGCTTGTGCTCCAGCTGCATTTGTAATTAAAGAGCTATAAAATAAGTTAGCATAACGAGCTTTATAGTATGACATTTTCATAGTTAAATATGCATAACTAACAGCATGAGATTTATTAAAACCGTATAGTCCAAATTTTTCAATATTGTTATAAATAGCATTAATAATTTTTTCGTTTATGCCTTTTTTAATGGCATTTTCAAAAAATATGCTTCTATATTTTTTGATATCAACTTCACTTTTTTTGCTAATTGCTTTACGCAATAAATCAGCTTGAGCAAAAGTCATTCCGCCAATACTTTGAGCTATTTGCATAATTTGTTCTTGATAAACTATTATGCCATATGTGTTGGCAACAACTTTGTCATAATCAGGATGTATTTTTTTAATTAATTTTTGATTTTTCTTATTAGTTGCATAGTCATTGATAAAGTCCATAGGTCCAGGTCTAAATAATGAAATAATGGCATATAAATCATCAAAAGAATTCAAGACAACTTTTTGAATTGTCTTCTTCATGCCCGGAGATTCTAATTGAAATATTCCTTCAGTAAAACCTAAGTTGAGCATTGTTAAAGTTTTAGGATCATTTAAAATTTGAGGATCGTTTTTTACTAAATAATCAAATTTTTGGTTTTCTTCTAAACTTTTTTCAATTAGCTGAATTTCAGTTAAAGTTCTTAAACCTAGCAAGTCAATTTTTAAAAGTCCATAACTCTCTATGTAATTCATTGGTACTTGTACCTGTTGGTAATTGTCTAAATTGCTTAAACAAACAGGGACATAATTAAATAATTTGTCCTTAGCAATAATAAAACCTGCTGCATGGAAACTTTGTTGACGAGGTAAGCCTTCGATTTTAAGTGCATGTTTATATAACTCAGGATAATTATCCATTTCAATTTTAAAGTCAATATCATTTTTATAAGCATCAGTTAAGTTATCTGATTTAATAGTTTTACTGATGCGGTTGATGTCAATTAAGCTAATTCCCATAACTCTGCCCATATCTCTAATTGCCATTTTAGCACCAATAGTTTGAAAAGTTGAAATAAGTCCAGTTTTATCTACTCCATATTTTTTTTGTAAATATTCAAAAACTTCAAAACGACGATCATCTTGAATATCTATATCAATGTCAGGCCATGAAACTCTATCAGGGTTTAAAAATCTTTCAAAAAGTAAGTTATATCTTAATGGATTAATTTCAGTTATATTTAAAAGATAAGAAACAAGTGAACCTGCTGCACTACCACGACCAGGTCCTATTGCTATTTTTTGACTTTTAGCTCAGTTAACTAAATCTTGAATAATTAAAAAGTAGTTAATATAACCAAGTTTTTTAATGACATTAAACTCATATTCAAGTCTTTTGTTTCAGTCTGGATAATTTTTTAATTCACTTGCTAGTCTTTTAGCGCCTGCTTTTAAAAGTGAAACAAAAAGCTTTTCATTGGTAGCTTCATCATTATTTTGAAAAGGAGCTAAATGCAAGGCCTTTTTAGGAAAAATAACATTGCATTCATTAATTATTGAATAAATACGGTCAATTATTTCGCTATCTACATTAACTTCATCAAAATAGTTGGCATAGTGTTTAGTTTCTTCTAAATTGCCTAATTTTTGTAAGTAATTTAGAGTTTCATTATCACTAGCTTCAAAAAGTTTATTTTCTTTTATTAATATAGAGTTAGGTAATGAAGCATCTGAAGAATTAATATAGTAGTTTTTACTTGCACTATATTTATTATCTTTAGTTTGAGCATAGTAACCATACCTTGGATGATCTAAAACTATTAAATTGGGATTTCATAATTCACTAATTTCAATAGCTTTATTAATACTTTTTTGATAAATTAGTTGATTAATTTTTTGGTAACCTTGGTAGTTTTTCGCTAAGACTATGAAGCGATAATTGTCTACATCTAAGTCCACTCCAATGATACCTTTGATATCATATTTTAAGCATGCTTCCATAAAAGCACCTAAACCGAACATATTGTTTCGGTCACTTAAAACTACTGCAGGAAGATTGTGTTCTTTTGATAATCTAACTAAATCATCTATTCTAATTAAAGAATCTAAAAAAGTGTATTCACTTGTATTGTATAAATTGACGTATTTTTTCATATTTTAATTATATTTATTTAATTAATAAAGCTAAGTAAAAGAATTATTTTTATATTTAGTTGTTAAAATAGTAAAATTATTATATACATTTCGGAGGTGTATTATGGCAAAAAAGATGAGTAAAGGTGCTATTATTGGTATATCAGTAGGAGCTACAATTGTAGTAGCTAGCACAGTAACCGCTGCTCTTGTGTGTACATTGCATGGAAAAACTGTTTATAAAATTGAATACTCTAAGTATAAAAGAGCTAGAGAAAAAGGCAATATTGGAGACAAAATAGCTTTAGCTACTTTAGAAGGACTTATTGTAACAGGTGAAACAACTTTGAAAGAAATAAATGGAACAAACCAACAATGATTGGATTTAGCTAAATTTGTAAAAGACACTAGAAAAGAAATAATAGGAAATAAATCAAAAGCTGATTTTCAAAAATTAATTGATGATTTTAAAAATTTTTTAAAAAGTTTAAAAGTAAAAAAATAATAGGCTGATAATACCTATTTTTTGTTTGCTTTTAGACATAATTGTTAACTAATTTAGTTAAAACAAAACGAATGTCAACTTTGTTTGTATTTACCTTTGGAGAAAGATAATTTAAGAATACTTTGCTTGTTTCAAAATGAATTTTAGTTGGTTCAATTTTCTTTAAATATTTATTCATTTCTTTTGCGTCTTTAAATTTTAAAAACATTGCATGATTGTCAAATGATTTAAGTGATGTTGATGAAAAATACTCATTAAGTATTAGTTCACGACCTAATAATGTGTTTTTAATTGATTGTGATAAATCAACTATTTTTTTGTCGAAAACAACTTTTTTAATGACCTTTTCGTAAAATAGGTTAAGTGATTTTAAACCAAATTTTGCATTGTTTTCCAAAGCTGTAAAAATGTTTTTGAGTTTTGTTCCTTCATAAATATCAAGCATTCTAATTGAATAATTTAAGAATTCACTACGATCATAAAAAGCTGCAAACATATTTGAAATTAAAAACAACTCAAACACTTCTTTTAGTTTAACAATATTTCATTTCTTTCATTTTGACAAAATAAGTGCTAAGTCACTCAATATGAAATATAAAATAGAATCACTAAGTGTTTCTTCATTATTTACAAATTGTAATAATTTGAAAATGCTTGATGACATTAGTCAATATCTATTAGGTATATTTACACCACGATATTTTTCAACTAAACTAGTTGGTATTAATTTTGCATATTCAATTTCAACTTGGTTATTGTTAATTTGCCCCACAAAAAGATTTTCTTTGTTTATTATTGCTTTATATTCTTTAATTTTGAAAACATGATTTCTAGTCTTTTTGTCTGAACTAATAAAGTGAAGTTTATTTAATTCACGATAAAAGTTTTGATCTAATCACATTAAAGCTGTTGAATCTAATAATGTAATCTTTTCTTTATTAGTAACTGATTGGATAGTTTCTTTCATATTTGTGTCTAAATCAAATATTAATCTTTTTAATTCGTTATATTCTTTTAATTTAAGATTAACTTTTAAATTATTAAGCATATATTTGTAACTCTTCAAATAGTTGTAATCAAATGTTATTAAAAAGTTATAAAGGAAAAATCTCTTCAATATCAAATAAATAGATATTCCAACTACAAAAATACAATACAATGAATACAAAACAACTGTTATTACAAAACTATTACTTTTAATGAAAGGAAGTTTTCAATTATTTAAATAATGAAGTAAAACTCCCATAATAACAGATGATAAAACTAAAAGAAGAGCTATCAACCAATCTAATAGCATCAAATTATGAATTTTGTCTTGTGATCTTTTGTATTTTTCAAAATATGGTTTAAGTTTCTTAATAAAAAGCATATATCCTCGTTTTCTTAATGAAATTATATCAATTTATTATTTAGATAATAAATTCTGCAAAATTTTTCTTAAAAATGAAAAAAGCCCTATAAAACAGGCTTATATGCTCATTAATTCTTTTTCTTTGTTGATTGTCATTTTGTTAATGTGTTCGATTTTTTTATCAACTTCTTTTTGAATTTCATCTAAGTATTTCTTTTCTTGATCTTCTGATAATTCTTCATCAGCCTTGATAGATTTGTTAACATTTTGTCTTGCATTTCTAATTGCAACTTTAGCATTTTCTGTAAATTTTGCAAGACTCTTAACCATATCTTTACGTCTTTCAGTTGTAAGAGGAGGAAAAGTTATTCTAACTTGATTTCCTTCATCAACAGGATTTAAACCTAAGTTAGCATTTAAAATTGTTTTTGCAATTTCATGGTTGCATGAAACGTCAAAAGGTTTAATTAATAATTGTTGTGGTTCTGGTACTGTAATTGAAGCAAGTTCTTCAAGAGGTGTCAAAGTATCATAATAATTAATTCTAACTCCCTTAATTAATTGAGGGTTAGCACGTCCAGTTGAAATTTTTGATAATTCAAAAGCATAATGATTAATTGCTTTTTCAGCCTCATCTAAAAAATCTAATAAATATAAATCTAATTCCATTATTTAATTACCTCTGTGTGTTCAATTTTACCTTCAAGAGCTTTGACAATAGCATTCTTTTCTTTAATGTTGAAAACAATTAAATTAATATTGTTGTCTCTAGCCATACTTGTGGCCGTTAAGTCCATGACTTGTAATTTTTTGTCTAAAATTTCATCATAAGTTACAACATCATATTTCTTGGCATTTGGATTTTTCTTAGGGTCTGAATCATAGATTCCACTAACACCATTTTTACCCATTAAGATAACTTCGGCTTTGATTTCTGAAGCAAATAATGTTGCTGCTGTATCTGTTGTAAAGTAAGGACGGCCTGTACCGCCAACAAAAATAACTACTTCACCTTCTTCAAGGTATTTTAAAGTCTTTTCATTAATATAATTTTCTGCAACTTTTTGATCAATTGCAATTGATGATTGGATTCTAGTTTTTAATCCAACTTTTTCGAATCCACTTTTAAGAGCTAGACCATTCATGATAGTTGCCAACATACCAATGTAGTCTGCTCTATTTCTAGGTATTCCATTCTTTTCAGCAGAAGCACCTCTTCAAAAGTTACCTCCACCAATAACAACACTAACTTGAACACCTTTGTCAATAATTTTTTTAAGCTGTTTAGCAATGTCTTCTACTAAATCATAGTCAATAGCTAAACTTTTTTGTTTATTTACTAAACCTTCACCTGAAAGTTTTACTAATATACGTTTGTATTTCACTTTTTTTCTCCTACAAATTTAACTTTAATTTTTTATATTATAAATTAATATCAAATAATTGTGAGTATTTTTCTTTACAAATATTTTGTAAAAAGCAAAAATACATAAAAAAATATAGAAATTAAAATCTATACTTCTTTTAATCATTTATTTTTTTATTTTATTTTTAAAAATTGACTAAACACAGGAAAATATTCATCATTGATTTTTGCCAATTCGACTGTTTTTCAGTTTAAATCAGAAAAAAATCTAAAATCGTCATGGCAAGAACTCAAGACAATTAATCTTAAAAATCTGTCTTCTTTAATTGTATTAATTAGCTCTATTAAACCTAATATTTTTGACTTACTTCCAAATATTTTTGTTGCTAAATTTATCTTATTTTTTCCTCAAAAAAGCAGTCTATTATGAGTTTTAAAACTATATAAATTTTTGCATTTTTCTTTACTTGTGCCAACAATTAAATCAATATAAATTCTTTTATTTTGGCATTTAAAAAATGGACTTAAATCTTTTTCATTTGTTTCATCATTTGTTAGAAATATTGAATTATTAAAATATTTGAGGTCAAAAAAATCTTTTCACCACAAAGAAATACGAAAGTTATCAGGTGTAACTTTTTCATTTTTAGAGATTACTCTTGCTGTCATTGGGCTTAAAGAATATGGAATTTTGTTAAGTTCTAATAGTTTTTTAAATTCATTTCAAGCAATCCAAAGCTCATCTTTTGTCATAAGAATAATTATAAATAAACTTTGAAAAAAGCATTGTTTTTTTTACATTTACTATATAATAAATTAGATTATAATTTTTATATCAATATTAATTTTAAATATATATTTAAATATATGGAGGAAGCATGGCTAAAAGAAAAGAAACATTTTTCGAACGTTTGACAAGAAAAAATGCCGAACACGAATATAAAAATCAACAAGCAATGCCTAAAAAGAAAAAGCTAAAAGGTATAATCCTTGGAACACTTTTAACATCTGCAATTGCAACAGGTATCATTGTTCCAGTTGTAATTAATTCAACAAAAGTAACATATAAAGACGCACTCAAAGATGAAAGTGCTGTTTTAACATTTACATTACCAGGATCAAAAGAAAAACATTCTATTACAATCAAAGAATTAAAAAATTCAAGTAAAACTGAAGATGCTAAAGATCCAAGTAAATTGTTACAACAAATTAAACGTCTTGCGATGTTTTATCTTTATGATAAAGAAGCAAAAGCCTCAGCTGAATATCAAAGATTATGAAATATGTCACGTGAAGGCAATGAAGAAGAAAATACTTCATTCAAATTAAAATCAATTGAAGAACTTAAAACAAAAGAAAGAAACAAGATTCTAGATATTCAAGAAAACACCAAAAGAACAGCCGGTTATGATAAATGAGAAGAAAAATTCAACAAAATTTTAGTTTCAAATTACAATGGAGCTAAAAACATTGATGAAGCAGTTGACTATCAAGTTTTTAAAGCTATTGAAAAAGATGCTTTAAGACGTTTTGAACTTTCAACAGTTGATATCAATAAAATAGTAAATCGTAAAGCTAACCAAAAGATCTATGAATTAGATAAAGATGGAAATCCAGATACAAGCAAAGTTTTATTTGACACTGGTCAATTAGTTTTTAACTACTACAAAGAAAACGAAAACTATTTCAAAATTAAAGATGATTACATGTCATTTACAACAAATAGTTATGTTAAAGCATATAAAGATGCATCTGCATTTATTGATGAATACTATAAAAATGATAATCCATATGCATTCACACAATTTACGATTCCAGGTGTTGCACCTACAAAAATAGCTAAAAAAGGCGAAAAAGAATTAGAATGAACAATTGACAGAACAGCTTTAAGAAAATTATTATATTTCTACCCAGCTGATGAAAAACACGGAAAAATTCAATCTTATGAAAAAATCTTTACAGACTTTAAGCCATATTCACATTACTCAAAGATTATCACAGATGCTAACAGTACAACTAATTTGCCTCAAGAAATTGTTGACTATACAACATTTTTAAGTTGAATATCAGCCGACGCTGATGAATACAAAAATAATTTTGGAACTAAGGGAATTCTTTCTCTAACCAATTTATTAAAATCAAATAATGATGAAGTTATTAAAGGTTTAATAACAATGCCAGAATTAGTTTATGGATCAACCTCTGTTCCCGAAATTGATTTAAAAGCATTATTTGAAAGTATTCAATCTAAATTAGAAACACTATTCCCTGAATTAGTTCCATTAAAACCAAATTATGATGCTACAGCTTCATTAGAAGAAAAAACTAAAAAAGTTGGAGATTACAACAAAAAACTTCTTGACATCATGAGAGATTTTGATAACAACAAGAAGAGTGGTTTATATGATGACAAATTAAAAGAAGAAATCATACCACTAATTGCTAAAGCTTTTGAAGGTGCTGACAAAAAAATAAGAACATTCTGAAAAGTTAAAGGTTTAGATAATGCTTACCTTTATTTAAATAGTAAAGGTGCTACAATAGTTTATTGTCAATCATTAACAGATGCTGATCAAGCTAAAAACAGAGCTAAAATGCTTGAAATCTTAAAAAATGATTTTATTTTAAGCAAAAAATACAAATCAATTAATGCAACAAAATACAATGCATTAGATGTAATTTCTAAAAACTTAAAATCAGATTATTATGTAAATAAAGTTTTATTAGAAGATGCTGATTTTGTTAAATATCTCAAAGAACAAGAAAATATTTATGCACTTGATGCACAAGGCAAAGCAGTAAAAGGTGTTAAATATTCTGACAAAGATATTGAAGAATTGAAAAAATCGAATGAAATTAAATACAGAATTGATGCTGAACAAAACGCTCTGGATGTTAATAAGAAATTAACAACTTGAATTAAAGAACGCGCTAAAAATGAAAAAGATAAAGACTTGTTATTTGAAGGCGGTTACAAAGTATACTTTACAAACAATAACAACAGCAAAAAAGATGAAGTAACAAAAATTATTAGTGAAAAAATTAAACAAATTTTGAAAGTTTTTAACTAGGAAGGAGAGTGCATAATGAAAAATAAAAAATTATTATTTTCTTTAGGAAGCATTAGCACTTTAGCAGCCTTCCCTATAGCCGCTGTTAGTTGTGGTCGTGTTGTTGAAACTCCAGCTAAAAAAATTCAAGATGATTTCTTAAAAGCTGGCGGCAGAAAAGAAGAAATCAAAAACATTTGAAGACAAATAACAATTGCTTCTTTATATAAGATAAACGTAACTAATTCATATAACACTGATAACTCTGAATACATAAAAACATTTAATGATACTAATTCACAACTTTATAAAGATTCTTTAGAAATTTTTAAAGTTTATGCTGAAGATAAAGTTTTCAAAAATGGAGCTGATGGTTATTACTTTTATGAAAAATCATTAGATTGAAAGAAAAAAAATTACTTTACTAAAGATCAAGAATCAAAAATATCAAATTTATTAAAAGTTGGCGAAATTCCTAATGAAGAAATTTTTAGAATTTTTTGAAATGCTGAAAAAACAGGAATTAGAGCCGAAATTCAAAAAATACTTTTAGTCCTAAAGTACTTTGAAATAAGTAATAAAGAACAATTAGCCAAACTTGATGACAAATTTAAATACAATTCATCATTCTTAAAATACGAATTAGAAAACTATAATTTAACTAGATATGCAGTTCAAAAGAAACCTGTTCAACTTTGAAAGAAAACTGCAAAAACTAAAATTACATCTGAAGAAGATTTCTTTGTTACTCAATTAAAAACAATTAAAAGTATTGACACATTTAATACCTTCATGAAAGGTTCGGAAGATGAAAGTAAAAAAATAAGTGACATTGAAAAAATTACTGATAATGCATACGAAAGTCAATTATTAGGCTATCAAGGTATTAACTTAAACTTCAGTAATTACAACTTGAAATGAGATGAAAAGAATTTACAAAATGCTGAAGCTAACAAACTTTATGGTTTCTATACACCTATTGAAGATAGACTAATTTTACAAGATGATGAATTATTTAAAAAAGGTTACAGTCCATATAAAGGCTCAAAATCAGACAAAGAAATTGTTGCTGCTTACTTAAACCAAATTGCCCCTATTGGACAACCTGAAGTTGAATTACCAAAAGAATCAAATGAAAAAGAAACTGAAAAAGTTAAACTTCTTTCATTCAAAGGCACAATCTTCCAAAATCATCTTAAAAAATTAGCGATCTTATTTGCTTTAAAAGATTCTTCATTAGTTGATACAGCAGAAACTGCATTCACTAAATTAGGTTACAAACTTAAAGTAGAAAATGATGCATTAAAAGAAGTACTTAAGGGTGAAAAATTTGTTGAAGGCGAAAAAGACAAAGACTCTAACAAAAAATAAGGAGTCAATTATGCAAAACATATTTCAATTAATAATTAACAAAAAAGCAGAAGCAAATATTATCTTTGAAGATGATAAATGTATAGCTTTTTATGACAAATTCCCAATTCAACCCGGACATTTCTTAGTGGTTCCAAAAAAACACAGTAAAAACATTACTGAAGCAGATGATGAAACAGCTGCACATTTAATAAATGTTGCTCGTAAACTTGGAAAAAAACATGTATTAGATAAGAAAATTGCAGGCTTCAAAATAATAATTAATACAGGCGAAGCTGCTGATCAAACAATTTTCCATACACACGTACACGTAATTCCATATCGTGAAAAAGCACAAGATAACTAAAAAGATGGCTTAATAAAAAAGTCATTTTTTATTTTTTAGTTTTTAACTAAACAAAGTATGCTAAATTATTTAATTTTATAAAAGCTATATGCAAAAGTGTATTTTTGACTTTTTGTACTATAAATAAGTAAAAAAATATAAAATAATAAAAAATATAGTATGATTATTATGCTATAAATCAAGAAAGGAAGATTATGACCAAAAAAGAATTTGTACAAAAAATTGCTGAAATGTTAGAAGTTCCAGTTAGAGTAGCAGATCAATACTTTGATGCATTTCTATTCGTTTTAAAAGAACAACTTATAGCTGAAGATAAAGTTCAACTTTCAGATTTAGGAACCTTCGAAACAAAAATTAGAAGAGGACGTGAAACAATTAATCCATTTACAAATGAACCAATTCAAGTGCCTGAAAAACGTGTTGTAAAATTCACACCTTCAAAATACTTAAGAGATATTGTAAACTTCTAATATCAACAATCACTAATAAAAATTTTGAACATAGGGCAAAAAGTCGATTATACCTGGAAAGGTTAATTCGATTCTAAAACCTATGTTTTTTATTTCTTCATAAGAAATAGATTTATTCATATGCTGAATTAACTCACTTGCTTTAACAAGATAAAACTCATCACAAAAACTAAAAAATATAATAAAGAAAGCTAATCCACTATTACTTTCAATATCCAATAAATACTTAATTTGGTGCCGAGCAATATTATTTGCTGGCAATCTTTTTTCATTTGTGCTTTTTGCTTCAAAAGCAACAAAAAATCCTTTATAGCAACCGATGTAATCAACTGTGCTCTTCTTTAATATATAAGCCTCATCCAATTTCAAATTATCATTTTCAACCTTCTTAAACTTAACAGGTAAAGACTTCTTTTCAATATAAGCTAATTTATTTTTGGCATAGTAATCAATTGTTTTATTAATTATTTTTTCTAATAACATTCCTCTATTTTTTTGCATGAAACACCTCAAATTAAAATTAACTTTTTTATGAAAAACATATCAAAAATGGAAAAATTTAGGAAAAATAGACTTAAAATACCAAATTTTTCCTAGAATCAACCAAAAATTAAAAAAAGCTAAAGTATAAAATGGAATGAAATATAGAAAGATAATATTAGGAACTTTGTTAGCAGGCACAATTGTAATTTCAACAAGTACAATTGCTTTTAATATTCAAAGAACCAAGGTTGCAAAGGTTAAAAGCAATGATCCTGCATCAATAGGATCAAAATTTTGTATCAAAGTATCCGGTGCAGTTGCCTTTGCTAAAAATTATTATTTCGAAAAGCCTATTTATTTAAAAGAATTATTAGATATGGCTCAACCTTATGAAAATGCAGACTTAAGCAAAATTGACTTAAAAAAAGAATTAAAAGAAAATCTTAATATTTACATTCCATATCAAAAAACTAATAACCCTTTTTTGTATTGAAATAACTTAAGTAAAGAACATTTTAAATTGTTAGTGGATTTTGGCATTAAAAAATCAATAGTTAACAAGATTTTAGATTTAAGAGAAAAGAAAAATCAAATAACATGGAACGACTTAGAAAATATTTCGGGAGTTGGCAAGGTAACCTTAAAGAAATTACAAGGAATTTTAATTCTGTAAGTCTTTTTCTTTTTAGTTTACTCTCACCTGTTTTATTACACTTAGTAATAAATGCTCAAGAAACCAAAATATTTTGAATACTATTATTTTTAATTTATGTTACTTTCTTAGCTTTAATTAAGCCTAAATATTTAATTTTAGTAGCAATGCTTTTGACTTTATATTTAAGTTATTTTTTATCTAAAACAAAAATTTATTATGAAAAAGGTATTTATAAAATTGAAGGATCGATAAGCGTCCTAAGCAAAAAATATTTAGTTATATCAAATGAAAATAATAATATTTTAATTTACACAAGTGATTTAAAAAATAGCACTCCTTTAAGTCTAGGGGACCAAGTAGAAATTCAAGGTGAATTAAATCCTATTGAAATAGACAATAGTAAAATAGCAACATTTTATTTGCAAAATAATATAAATTATTTGCTAAAAAAAACAGTCATTTTAACTGTTATTAAACCAGAATTTTCTGTTGAAGAAAAAATAATGAATTATTCAAAAAATCATGGTGAATTATTTGATTCATATTGAAGGTTGTTAGTTTTTGGATCCTATGATTCAAAAGAATTAGTATTGAATAAAGTTAATAGACTTAATATTGTTCATTTAATTGTTATTTCAGGTTTGCATTTTGACTTATTATTCTATTTAATTTTGTTGATACTAAAACCTTTGGATAAAATTAAAATCAAAAGTAAATATGTGGCTTTTGCCCTCATATTCTATTATTTAACATTACTTGCTAATCCTGTTTCTGCTTTAAGAGCTTATATTATGCAAGTTGTTAAAAATTCAAATAATCCTTTTAGTTATAACAACAAATTTAAAAAATATGATGGTCTAGTAATTTCAATTTTAGTTTTATTTTTACTTAAAAATAATTACTTGTTTTCTTTATCATTTATTCTTAGTTTTTCATCAACTTTTACAATTATGTTATTAGTGCCAATGGTCAAAAGTAAAAAATTAAACAACTTTTTAAAAACATTAATTTTGATGACTTTAATATATCTTTTTAATATTCCAATGCTTTTATATATTAATGAATATTGAAATTACTTCGGATTATTACTAGGAATAATTATGGCTCCATTATTTCAATTTTTTCACTTGTGTTCTTTATTATTTTGATGAAGTCCAACAATGCTGAGTTGAATGTATTACTTGTTAGATAATTTGTTATATTACTTAGTTGAATACACTTTTGCTTTAAAAATAAATATAAGTATCAATTGATGAATATTAATCTTTTGAAGTAGCTTTTGATATTTAACAATCTTTTTTATGAAGCTAAAATCTAATATAAAAATTTGAAAAATAAAAACAGCCTAAGCTGTTAAGGGTCATCACTGATGAAATGGCGTACCCAGCAGGAGTCGAACCCGCAACCTTCAGGGCCGTAACCTGACACTCTGTCCAGTTGAGCTATGGGTACAAAATGGAGACACCAACGGGATTCGAACCCGTAATCAGAGTTTTGCAGACTCATGCCTTGGCCATTTGGCTATGGTGTCATCTGCAAATAATTATATAATATACGACTAAAACTAATTATACAATTATATTTTTTTATTTAAAGAAAAAAATAATTACTATTTTTGTCAATTAAAACTGCAAATTCTCCATTAATAAATTTAATATTTTTTGCTTTTTAATAATACCACAAAAAATATTTATTTTGTATTTTATTTATAGACTTGGTATTTTAAGCCTTAAAATTGTTTTTCAATTTTGATAAAACAACTATAAAACAAACTTTTAGTTATATTTTTTAACAAAATCTAAAAGTAATTCAGGTTTCTTGTTTTTATATAAAATATCAATTATGCTTTTTAAAAATACAATTTTAAGATCTTTATTTTTCTTTAGAATATTAGCTAATATTTTAGCAGCATGATATCCTTCAATAGTTTTCTTATTATTTTTTAAAGTTGCTTCCAAGCCAAATTCTGCAACTTGTCTACCAAAAGAAAAGTTACGGCTTTTTGTACTTGAACAAGTTAAAAAGATATCGCCAATTCCTGCTAATTCAAATCCCATATTGTCATTTGATTCAGGAAATATTCTCTTGTAAACCATGTGAATTTCTTTAACACCAATTGAAAGCAAGGCCGCCATTGTATTTCGATATGGTCTTAAATAATCAATCATTCCAATTCCAATAGCTAGAACATTTTTTAGAGCAGCAAACAATTCTGAACCGTGTTCATTTTCATTAACAACAAGTCTAAAAGTGTCATTATTAAATAATTCAGAAACTTTTTTTAAAAATCTTGTTTTTGGACCAACAACATTAATCATGGTTAAAACATTTTCAAAAACTTCAACAGCAAAAGAAGGACCAATTAAAGTACAATAATTTTTTAAATTATTCGCAAATTGTTTGTTTAAAACTTCTGAAAAAAATTCATTTGTTTTTTCATCAATACCTTTTGCAACATTAATAACATTTATTTTTTTATTTTTGATTGTTTCCTTTATTTGTTCTAACACAGAAGTAATTGCACTTGAAGGTACAGCTAAAATTAAATAATCAAATTCTTTTAAAGCTTCATTTAAGTCCAAAGTAGCTTTTACATTAAAAGGATTATTAAATAAAGCATCTTTAAAATATTTACTATTATTTCCTTTGTTAATTGAATCTACTTCACTTGAATCAATTCCTCACATTAAAACCTTGTTATTATTTTTACTTAAAACAGTTGCGAGTGCTGAAGCTCAGGCACCTGTTCCGATAATTGTTACTTTTTTCATAATGCTCCTAAATTATTTAACTATTATTGTGTCATCATCATTTTTGTTATTAGAATTTTTATCACTGTAATCTTTTTTGCGTTTAAAAGCAATTGTACAATATGCTTTATAGTATTTAATAGCTTTTTCTTTTTGATCACTTTCATAATACTTGATGGCTTTCATTATTAAAATTCAACCAGATAATTGCAAAGTTCCAAATAATGTGAACACTGTGAAACATAAGAAAATTGCAACTTGTGCGCCAACATTAAAATAACCAAAGCCAAAAACCATTGAAAGTGTAAATCAAACCGCAAAAAGTCCAAAAGCAATAAGATAGCAAAGCGTTACTAAAATAATTATGAACTTTTTGGCATTTTTATAAGCTTGTGCTTCTTCATGATAGTTTTTACAAACAACAATTAATTCTTTTTCATATTCTAAATCTAAGTCCATAAATCTCCTAATTTTCTAAGCAATCGTATAAAGCTAATATAGTTTTTAAATCTAATTCTTGAATTCTAGTTAATTCTTTTAAATTTAATTTATTATATGCAATTTTAATTTTATCATTACTATATTTTGTAGCTAAAGCAAATGATAATTTTTTTCTTCTAGCTAAAAAACATAATTTGAAAAAGTCTTTTAAATTTTCATAATTATCATTTGCTTCATTTTTAAAAGTAATAGAAACAATAGCAGAATCAACTTTAGGAACTGGATCAAAATAAGTCTTCTTAACAAATAATTCTTTTTTAACATCAGCACAATATTGAACTGTTATTGAAAGTTTGCTGTAATCATTTTGATTAGGTTTAGCCACTAATCTTTGAGCCACTTCATTTTGAACCATAAGAATAGCTTTTTTAAAATTGTGTCGATAATCTAATAGTTTAAAAATAATATCACTAGTTATGTAATAAGGAATATTCCCAACAACAACATAGCTTTTATATTTTTTCAAATCAGCTATTAAAAAATCTCCTTGGACAATTTGATTTTGATTTAAGATATTATTTTTTTGTAAATAATCAACCATATCTTGATCTATTTCAAAAGCAGTAAATTCTTTTGCTTGTTGATTTAAAATTTTAGTTAAAGCACCTCTTCCGGGGCCTATTTCTAAAATCTTTTCACCTTGTGGTTTAATCAATTCAATTATTTTGTTAATAACATTTTGATCTTTTAAAAAATTTTGACCATATCTTTTTTTGGCTTTTAATTTATTTTCTTGAAACATTTATCTTAAATAAATCCTTTGCATTTTTTAAAATCTGGTCAGTAAATTTTTCAATTGGTATTTTTTTAATACCTGCAATAAAGTGAGTTGTTTGAATTACATAATTTGGATAATTTAATTCACCTCTTTTGTGAGCTGGAGAAAGAAAAGGAGCATCAGTTTCAGTTAATATTCTATCAATTGGCAATCAATCTAAAACTTCAATAGTTTCAGTTCCATTTTTATAAGTTGCCACTCCGCTAAAAGAAAAGTAACAACCTAATTCGTAAAACTTTTTGGCTCAATATAAGTTACCACTATATGTATGAATCATAAAAACAACATCTTTATAATATTGTTTAATAATTTCGTACAAATCTTCATATGCATCACGCATGTGAATTACTACTGGTAAATTGTGTTTTTTAGCTACTTGAATTTGAGCATGCAATGATTCAATTTGAATATCTTTCTTTTCTGGTGTATAAAAGTAATCTAAACCAATTTCGCCAATTGCAACAACAGAATCATCTAATTGCTTTTCAATAATTTCGCCGTCAACCTTTCCATGACATTCATTTGGATGAATTCCAATAACAGGAAAAGTATATGAAAAGTTTTTGGCAATTTTAATAACTTCTTCATTTTCTTGTTCATTACATCCGGTAATTAATAAAGCTACTAAACCTTTAGCATGAGCTCTTTCAATTACATGATAATTGTCATCATAGTATTCTTTTAATGGATGAGTATGAGCATCAATATATTTTATTGACATAGTTGTTCCTTTCTTATTTCTATTTTCCTAAACAAAAGTTTTTAAACATTTCGTCAAGCAATAATTCATTGTCAGCTCTACCTGAAATATCAACTAAATCAGCTCAAGCTTGTCTTACATCAATAATTACTACATCAACATCAATGCCATTATTTATTGATTCAATTGCATCAGAAATACTTGAATGAGCCTTTTTAACCAATGCTAGTTGTCTTGCATTAACAACGTATTCTTCATTGTTAATTTCATTCTTTGAAAAAATATTAACTAATGCTTCCTTTAAATCATCAATGTTTTTATTTTTAGCACTGACATAAATAACATTCTTTTTCTTTTTATTATTTTTTAATAAATCAACTTTATTAATTACTTTAATTAATGGTTTGTTAAATTGTTTAGCTAATTTTTCAACTTTTTTATCGTAATCATTTTCTAAAGTATTAGCTTCATTAACATGAATTACAACATCACATTTTTCAATTTGTTCAAAAGATTTTTCAATACCTATTTTTTCAATTTTTCGATTAGTTTTTCTAATTCCAGCGGTATCAACTAATTTGAATAATAAACCATCAATTTGATATGAAGCTTCAACAAGATCACGAGTTGTTCCAGCCTGATCTGTAACTATTGCTTTATCTTCATTTATTAAGCAATTTAATATTGAACTTTTTCCAACATTTGGCTTACCAAGAATAGCAACTTTAATGCCATCAAAAATTAATCTTGAGTTTTCACTTTCTTTGATAATTAAGCTCAATTTGTCTTGCAATTTTTGCAATCTAGAAATTAAATCTTTTGTCAAAACATTTTCAAAATCTTCATACTCTGGATAATCTATATTTATTTCCATTTGACCTATTAAATAGGCTAATTCGTCAATAAATGATTTAATCATGTTTGAAGTTTTGCCATCAAATTTCTTAATGGCTAATTCACTTTGTTTTAATGTTTTGGCATGTATTAAATCATTAATTGCTTCAGCTTTAATTAAGTCCATTTTACCATTCAAAAATGAACGTCGACTAAATTCACCTGGCAGTGCCATTCGAGCACCATTAGCTAATAATAACTCAAGAATTCTATTGGTTACTAATACACCACCATGGCAATTAATTTCAACAGTATTTTCGCCTGTAAAATTTTTATTTCCTATAAATCAAGCAATTAAAACTTCATCAATAATTTCTTTTGTTTTATTATCAAAAATATGGCCATAAGTTATTGTTTGATTTGTTCCTATTTTTCCTTTAAAAATTTTAGAAACTATTTCAATACTATCTTGACCACTAACTCTGATTATTGATATGGCTTGATTAATCTTACCACCTGAAGATATTGCTGCAATTGTGTCATTTATCATAATGTAAATATTTTACATTAAATTTAGATTTTTGTTTGTATCAATAAATTGACAAATAAAGGAGAAAAAAATGAATAATCAAAATTTTAAGAATTTATTAACTAAATATATTGAATTCTTTTATAGGTATAAAAGCATTTCATTATTAAAAAAGGCAAATAATGAATCACAATTTGATGTTAAATTAAGATTAGTTTTAACACAAAAATTTTTACTGCATTCACTAGGTTTGCAATACAACAAAAAGTTATTTAGAAAATATAAGAACAATAATTTTGAACTTTATAGAGATATAAAAAATGATCAACTAAATTATGATGAGTTAATTAAAGACATAAGCAAAAACAAAAAAGATTTAATTGACAAAAAATTAAACGCTCTTGAGATTTTTGTCAATATTATAAAAAATATTAATCAAGAAAAACTATTTACTTTTGCATTTGAATTTTTTAAATTAAAACCAAAATTTGATATTGATGGAGCTTATAACCATTCACAATTGATTTTAGTTACAAAAAGCAATAAAGAAAATCAAAGATGTATTATTTATGGAATTTTGGGAGATTTATATACCACCAATTCAAAAGATAATAATCATTTTTTAAACGAAAATAAAAATCTTATGAACACAGTTTTTATACCTATATCAATAAAAATCAAAAACAATGAAAATCTTTTAAATTCTCAAAATATACAAAAAATTGATATTGCATTTTTAAACAAAAGAAGAATACAAGAAAAAGAAATAAGAGTATAAGAAAACTTGCTAATAAGCAAGTAATCTTTTATTTATATTATTTATTTGATTCAATTTCATTGTTAATGTTTTCAGCTTCAACACCTACAATAATTTGAATTGAATTCTTTCCTAATCTAATCAAACCACTGCATCCCGCAAGTTTTAATTTTGCTTCATCAACAATATCTGAGTCTTTAACTTCATAACGAAGTCTTGTTGCACAGTTTGCAAAAGTTGTAATGTTTTCGTAACCACCAACAGCTTCAACAATAGCTTTTGCTTTTAGACTTAGGTTATTTAATTTCGAATCTGTTGGAGTTTCTTCACCAGAAGCACTTGAAGTTGATTTAACTTCTGTTAAAGCATTTGGATCAGGCAATTGGTTAGCACCACGACCTGGAGTTTCAAGTTTAAGACCTTTAATTGTATAGTAAGCAACAAAGTAATATGTAGCTGCACATGCAACACCAATTACTAAGATTCAACCAGGGTTAGCTAATACAGCATCAATACCTGTTTTGTTAGCTTTAATAATTGCTAATGATTTAGGAATTGATAATAAGTAATCCATTAAACCAGCTGAGAACCCAAATCCAATTTGAATTCCAAATGCACCAGTTATAAATGCAAATATACCAGTTAGAATGCAGTGGAAACCATATAATAATGGACTAACAAACATAAATGCAAATTCGATAGGTTCAGTAATACCTGAAAGAATTGATACTACTGCGGCTGCTCCAAAGATAGCCATTACTCTTCTCTTTTGCACTTTATCATCTGCAGTCATGTAGAAAGCAAATGCTAGAGCAGGAAGACCAAACATCATAACTGGAAAGAATCCTGATTGGAAGATACCAGCTGTGTTTCCTTTTGCTTTACCACTTAAGAAAATAAAGATATCACCAAATACAGGTGTACCATCAGGAGTTACATGTTCTCCTAATTGGAATCAGAATAAGTTGTTTGGAATGTGGTGTAATCCAAATGGAATTAATAGACGATTAATAACGCCATATACACCCATAATAGCAGCTGAAGCTCAACGACCTTTAGCTGCACCTGATAACACTTTTGAAAATGCATAAATACCATATCCAATTCATGGAAAAATAGCAGCATATGCAATGCCTCAAATAAGAGTTGAAAGAATTGCAAGAGCTGGAACTAATCTTCTACCCGAAAAGAATCCAAGAACTTTAGGTAATTGAACATCATTTGTATGGTTATAAATAGCTGCAACAATAAATCCAACAATAATACCATTAAGTACATTGCCAGACATTATTGCATTGTATTTGCCACCAAAGACACCTGCAAAACCATTAGCTATAACAAATTCTGAAGCAGGTTTACCTTCAGCTATAAAAGCTTCAAGTTTTTTACCATCTTCATTAAATTCGGCTCCAGGGAGTTTAAATGTGCTGTAGATTTTATCTACATATCCACCACTTTTTAATAAAAGTGAAAGTAATAGAATACTAATTAAAGCAGCAAAAGCAGCTTCACCACGGTTATCTTTAGTAAAACCAAATCCTATTCCGACGGCAAATAAAATGTACAAGTTGTCAAATACAACTGATCCGCCTGCTGTTATCATTTGTTGAACAAAGTTAACAAAGTTAGCTTCGACTTGTGAAATATCAGGTCCAGCTTTAACTGTTGGTAAATCAGCTCCAACTCTAAGAAGAATAGCAGCAATAGGAAGAACTGCAATAGGGAACATCAATACCCGACCAATGCGTCCAAGTTTTTCTAATAGTCTTTTTCCAAATGAAGGTCTTGATGATTGTGTTTTTGTACCAGTCATTATTTACCTCCTTGATTTTGTTGTGCTTTTTTTGTAACAGCTTTTTTATCTAAGATTGATAAAATAACTGTTCCAAAAATTGCAGCACATAAAGATATAAAGAAAAATAAGGCAACAATGGCTACGTATTTAGCATTTGTTGTTTTTAAAGATTCTGCAGCATCGAAGTTCTTAATAGAAGCTAAGATAATAATTAAAACCATAAAAACAAGAAACACCATTCAAAGTGAAAAAATAATCCACTTGTTTCGAGTCATGTTTTTGAACATAATTTTTCCTTTCACGTATATACGTTAGTGGAACCTTAATACATAGTTCCATATATATTTTAATATTTATATTAATTAGAACAATTTATAACGACACATAAATTTGATTAAAAAGCCTTAAAAATAGGCATTTTTTATTATTTTGACTCAAAATTTGTGGAATTTTTCACATCATTAAGTGCTGAAAAAACCAAAAAAAATGCGAATTTTGGACTACTTTCCAAGATTCGCAAAATATTATTTTTGATTATTTTTCAACTGCAAATAATAAGTTATTTACTAAAACAACTGAACCAGTTTGGTCGCCTTTTAGCAAATCACAAATCACAAATTTAATATTTAACTTGCCTTTAACAAATTCTAAGTTTACTCCGTTTGCATGAGGTTGAGTTTTACCACCATATTTATTTTCATAAAATGTTGAAATTCTAGCAACTTCGAATACTTTTGAAGTTAAATAAAATGCCTTAAATTTTTCAGTAAATTCAATAATTGTATAAGCAGATCTTCCGATTTTATGTTGTGCAGGATAAACTTGAGCAAATATATCACCATTTTCTAGTTTTTGTTTAGCACTAGCAATCCCATCTTCTTTAATTATAAAAGCACTTCCGTTTTCAACCATTGCTTTAATTGTTCTAGCTAAAACTAATGGCGCTTTTTCTTTAATAGTTTTAATTGCATTATCTACATCAGAAGGATTACCTTTAGCAGTTAAATCTACTCCTTCTTTTTTAAATTCTTCTACTAATTCCTTGAATTGTTTTAATTTTTCCTTGTCAGTAATTAGTGCAGAATATTTAGAAGCATCTGCATCCTTTGTCAATTTTTCCTTTAAAAGATCAGCTAAAGTTGCTTCATCTTTACTACCACATGATGAAGCAACAAATGGTATAGCTGTAATAACTGGAGCAATAATTCCTGTTAACTTTAATAAGTTTTTCTTCATAAATTCTCCTTTAATTCTATATATCTATTATATCAAAATGCTAAAAAGTTAAAATGGCAAATAAAAAAGCACTCATATTGAGTGCCTTGAAAAAAATGATTATTTTAGTTTTGCAAAGTATGACAATGTTCTTACTAATTGTGAAACGTATGACATTTCATTATCGTATCATGCAAAGATTTTGTAAAGTCTTTGACCATTTCCTTCTTTAACTTTTGTTAAAGTTGAATCGAATAATGAACCATAGTGTGAGTTAATAACGTCTGAAGAAACAATAGGGTCAACTACATATTTCATTGTTTCGTTTTCTGCTTTTTTGAATGCAGCATTAATTTCTTCAACTGTAGGTTGTTTTTTCAATTGAACTGTTAAGTCAACGAATGAACCTGTAATTGTAGGAACACGCAATGCTGAACCATCTAATAATCCATCAGCTTCGGGAACAACTAAACCAATAGCTTTTGCAGCACCTGTTGTTGAAGGAACAATGTTTTGAGCAGCAGCACGAGCACGTCTTAAGTCACCTTTACGGTGTGGACCATCTTGTAACATTTGGTCACCTGTGTAAGCGTGAACTGTTGTCATGTAACCGTTTAATAAACCAAATTTGTCTACTAAAACTTTAACAACTGGTGCTAAACAGTTTGTTGTACATGAAGCACCCGAAATAATTTCATCTTGTTTTGTTAAGGTTTCGTGGTTTACGTTGTAAACGATTGTTTTAACATCTTTACCAGCTGGAGCTGAAATAACAACTTTCTTAGCACCTGCTTCGATGTGTTTGTGTGCTAATTCTTTTTTAACAAAGAAACCTGTACATTCTAAAACAATGTCAATTCCTAATTCACCTCATGGTAAGTTAGCTGGATCTTTTTCGCAGAATACTTTAATTTCTTTTCCATTAACTTTAATAGCATTTTCTTTAACTTCAACCTTAGCTTCCAATGGGCCAAAAGCTGTATCGTATTTAAGTAAATGTGCTAACATTTTTGGATCTGTTAAGTCGTTAACTGCAACAACTTGAACTTCTTTATTTTGGGTTTCAAGTAAGTGACGAAGTGTTAAACGTCCAATACGACCGAAACCGTTGATTGCAACTTTTTTCATCTTTCTCCTTAATTAAAAAAATGTAAATTTATAAAAAGCTTTTTATACGCAATAGCGTTGTATTCATATGAATAAATACATTTTAATTATATATTTTTTTAAATAAAAAAATCATTTAAGTAAGCAAAAAAAATGAATCTTGTGCAAATGCTCTCACAAATAAAAAATAAAAGGCTTTGAGCCTTAAATTATTAACAAATAGTATTGCCTTCATAGGTCTTAATTTCTTCACTAAAATATTTAGCTAAAGATTTTCATTGTAAAATTACTTCTTCTTTTTTAGCAAACTTCTTATATTTTTCTAATGTACTTCATCTTTCCATAAAAATGAATGTATTATCATTTACTCAGTAGCCATCAAAAGATAAGTTGTATTCTTCTTTTTTAACTTCATCAATTCATAAACTAACAGTTTTTTCAAAAATCGATTTGCTTTCTAACTTGATTTTTACTTTTCTAAAAATTGCATAAATCATTCTTCCTCCTTTATTTTAATCTATTAAATAACAAATATTTCTCATCACAAATAACTTTATCAAATTTATTGAAATCATTAATTTTTTCAAAGTCAAATTTTTTAACTTTTAAAACACTTGCAACTTCTTTTATTTTTGTAGGCAATACTATTTGTAATGCACAACTAACAGCATAAATTCCATTTAATAATCTTACTAAAATTGGTTCCAATTCTTCTAATTTATCAGTTAATTTTCATGGTGTTGTTTCATCAATATATTTATTTAATTTAGCTGATAATTCAATTGCTTTTTTAAATCCTTTATCAATTTCAAAAGCGTCCATATGTTTTTGAAATTGAGCACTGAAATTCTTAATTTCTTTTTCAATGTCTTTATGTATTTTTTTACTATTAACACTATAAAGCAGACCTTTTGGGAAAGAATTGTGAATCATTTTTAGTGTTCTTGAAACTAAATTACCATAATTATTTATTAAATCTGCATTAACAACTTCTTTTAATTTTTCTTCACTAAAATTACCATCTTCTCCAAATATTATTTGAGTTGCAAAATAGTATTTAATCATCTCATGATGATACTTGTCAAATAAATAATCTGGGGCTATAACATTATTTAAAGATTTGGACATTTTTAGTCCATCTTTATCTCTTAAAAGTCCATGACTAACAATATGATTTGGTAATTTAATACCTAAACAATTTAAAACAATTGGTCAGTAAATAAAGTGAAATCTTGCTATTTCTTTGCCTAAGATATGCACAATTTCGTCACCATTTTTTCAGTATTTAACAAAGTCATCTTTTGGTTTATTAAAGTCGAAACCAAGTTTTGTTATGTAGTTAAATAAAGCATCAAATCAAACATAAATTGTATGTTTAGGATCATCAATTGTTGGAATGGCTCATTTAACATTTGTTCTTGTAACTGATAAATCTTCTAAGCCTTCTTTAACAAAATTGTTTGTCATTTCATTAACTACTTTTTTAGGCAATAAGAAGTCTGGATGTTTTTTGTTATATTTGCTTCATCATTTTTGAACTTCATTCATTTTGAAGAAATAACTTTCTTCTTCCATATTGATTAATTCATGTTTTGATGAAGGATGATAATACTTACCATCTTTATAAACAGCTTGATTTTTTGTCAAAAATTCTTCATCTTGAACTGAATAAAGTCCTTGATATGAACCTTTATAAATTAAACCTTTTTGTAAAAAATAAGAAAATATTTTTTGAACAGCTTGTTCATGAAATGATTCTGTTGTTCTACTGAAATAGTCATAATCGATTTCTCATTTAGCTCACATTTCTTTGTAACTTTTAACTAAATCATCAACATATTGTTTTGGTTCTTTTTTAGCAACTAAGGCTTTTTCTTGGATTTTTTGACCATGTTCATCACTTCCGGTTAAAAGCTTAGCATCATAACCTAAAACTCTTTTATAGTTTCTAATAACTCAAGCCATAATTGTACAATAAAGATGTCCGATATGAAGCGGACCTGAAGCATAATAAATAGGAGTAGTTATATAAAAAGTCTTTTGTTTTTTCATGTTTATTTACCTTCTTTAACTTTAATTGGTTTGTACAATTTTTTAATATTTTCTAAATATTCATGTTGCTGTTTGTCATTAGGATCATGCAAGTACAAGTTAGGCAAGAAATGAACACCTCAACCAGCTTGATATCTGCCTTCAACTAAAACTAATTTAGGTTTATCTTGCATTCTTGGAATAATGAATTGAACTCTTTTAGGCTCAAAATTATATTTTCTCATTAAGCAAAAGCAATCAACTAAGCGTTCAACTGGAATCACTAAAGTTAAGTAGCCTTTTTGCTCAATTAATTTTGAAGAACCTAAAATAATTTGTTCTAAATTAATTTTATACTCATGTGTTGCAATTAGTAATTCTTCACTAATATTTTTGCTAACTTTATTAGTTTGCATTGTATAAAAAGGAGGATTGCAAACTATTGAGTCATATTTACTAGCTGCATTTTTAATTTTTTCTAAATAAAATTCATTGAAATCTTGATTAATTATTTTGATTTGATTTTGCTTGTTGTTAAGTTCAATATTGTCTTGAGCTAACTTACAAGCTTTAGTTTGGATTTCAACTGCATCTATTTTTAAATTTGGATTTCTTTCAGCTATAAAAATACTGAGAGCTCCATTGTTTGCTCCAATTTCTAATAGGTTTTTAATTTTATTATTTAAAAAAACAAAGTTGCCAAGCATAATAGTGTCAACTGAATAGTTGAACATATCTTTGTCTTGCACAACATATAAATTAGAATCAAAACCTAAAGAATTTTTAACTAATTTTTGTTTTTTATTCATAATTATTTACCTGATTTTTTATGTTTGCGATTTGAGTTTGAATTAAGTTTTTTCTTGATTTCTTCTAAGTATGAACCATAGCATTTTGCAAGCACTGCATCAATTTTACCGTTAGTTTCATCAGCTCCAGCAATAACCACTTCTACTTGGTCTCCAAGTTTAAAAGTGTGTTGTTTACTTCTTAATTCAGTAAAGTTTTCATTAACTTCATAATCACCATCACATAATGTTGAAATATGAACTAAAGCCTCTATTTTGCAGTCAAATTGGACAAACATACCAAAGCGTAAAATACTAATAATTTGAGCTTTCATTGTTTGACCAATTTTGTTTTTGAAAAACTCAGCATATTTTAAATCATTTGTATCACGTTCAAGTTGTAATGCTTCTTGTTCACTATTTGAATTTAATTCTGCAACATTACTTAAAATGTTTTTGTAGTGAGTAATTTTACTTTTATCATTGTTAAAAACAATTTCTCTTAAAATCCTATGAACAACTAAATCTGGATAACGTCGAATTGGACTAGTAAAGTGACAATAGTGTTCACTTGCTAAACCAAAGTGGCCAATATTATTTGTAGAATATTTAGCTTTTTGCATTGTTCTTAAGAAAACTGTTTTTAAAAAGTCATCATTTCTTGTTTCTTTAATTTTGTTGATTATTTTTTGAAAACTTTTTGGATTAATATCATTTCGATCAATGTTTACTTTAATACCTAAAACATTAAGTACACTCAAAAATGAATCAATTTTGTCACTATCAGGGCTTTCATGAACACGATAAATAAAAGGTATTTTTCTTTTAGCTAAAAATTTAGCTACTTCTTCATTAGTTCTAACCATGAAATCTTCAATAAGATTTTCACTAAAACCAGTTGGCTTAACTACAACATCAATTGTTTTACCTTTATCATCTAAGACAATTTCGGGTTCTTCAATTTCAAAATCAATGTAGCCTTCTTTGACTTTATAATTTCTAATTATTTTTGCCAATTCAGCTGCTTCATAGAGCATTCGATTTAATTTCGGGTCATCAAATTTTTGATTTTTATCATAAAGTTCATTAACCCTATCATATGTGAGACGATATTTTGATTGAATTATTGACTGATAAAATTTAGCATCAATAGTATCACCATTTTTATCAATTGTCATTATTGCACTAAGAGTAAAGCGATCTTCATTAGGATTAAGTGAGCAAATACCATTTGATAATTTATGAGGCAACATTGGAATAACGCCACTAACTAAATATCTTGAGGTTCCTCTTTTTAATGCTTCAACATCAAGAGAGCTGTCTTCTTTGACATAATAACTAACATCAGCAATATGAACGCCTAATTCATAAGTTCCATCAGCATTCTTTTTAACATTAATTGCATCATCAAAGTCTTTTGTGTCATTACCATCAATAGTAACAATTAATTCTTTTCTCAAGTCAAGTCTATTATTAAGTTTTTCATCTGCAATACTATTTGGAATTTTAACTAAGTAATCTTTAACTTCTTGTGGGAAATCTTCAGGTTTATTTCTAATAACTGTTAAGGCTTTAACATAAGCCATTGGATCATTACTGTTAGCTATTTTTTTGATAATGTTAAGCTCAATAATATTATTTTTAAATGAAACCATTTGACCTAAAACAACATCATGAAGTCTAGCTTCTTTAACCAATTCTTTAATATGAAAATTAATTTTTTTGTAGTCATTTTTAAGTGGTTTAAATGAAAGAAAACCTTCATGCATACCTAAAATACCAATGAATTTATCACTGTTTCTGCTTAAAACTTTTTCAACAATTGCAAAAGTTTTATCATCTAAAGAATGAAAGTATTTTAAAACTGTTATTTCAACAAAGTCTCCATCTAATGCTCTATTAAGATGAATACCAGGTACAAAATAACTTATTTTTTCAGCTTCAGGATTATCATTTTCTTCTTCTACAAAAGCATACTTGCCTTCTGAAGCAAATCGAATTTTTCCTTGGACGATTTTAATTTTTTCTATTAATGAATAAGTTGAGAGTTTGCCATTTTTAATTAATTTTCTTTCATCAATTAATTTATTTAGTAATTCGGTTAATTTTTTGTTATCTTTATAACTTATTCTTAAGACTTTTGCAATATCAATGAAAGTTGAATTATCATGTTTTTTTAAAAAATTTAATATTTCTTGCGGATTCATAAGTTATACAATAATCCTTAAAATAATAGCTAAAATTAGCAATGCTATTCCACCAAATAACATTGAGTATTTTAAAACCTTTTTAAGTCCTCTTTCTTTTGAATATTTAAAAAGTTCCAAATCACCACTACCAACAAGTGCACCAGAAAAACCATTACTGTCTGGTGACATAAGCAATGAAATCACAATAATTGCGATCGATAAAATAGCGATAATTATTGATACAAATATAACTGCCATAATTCTCCTTTCAACCTAAAAACTTCTTATTATTATTAATATTAAGATTATAAAGGAAAAGTTGCAAAATTTATGTTAATTTTTAGCATAAATATTATTATTCTAAATTTTAAGTTAATTTTTCTACTAATTTATTGTATTTTTTATAGTGCTTTTTAATATTTTTAAAATTATTCAATATAATTATGAAGATAAAAAATATATGGAGTTAGTATGAAAAACAAAATAACCAAACACAATATGTGTGAAATTTATGAGTCATTTCGTTTTATTCAACCTGAATATAAAAGTCGTGTAAATTCATTAAAAAATAAAAATGAAAATGACGAATTTTTAATCAAAGCACAAATGGAAAGTGCTATTTCATTGAATGAATATATTGATTCAAAATTAGAAAGAAAAAAGATCAGAATTGCTAATCGTAAGATTTCAAAAATCTCAAGTATCTTTATTCCACTAATTGTTTTCTTCTTAGTAACTGTTTTAATTGCAGTGTCTTATATTGCTTATGTAGCAGCTAACTATATGGTTAAAGATGCTGAAGTTTATACACTATTTGGTATAGCTATATTATCACTTATTTCAGCAGCAATATGCTTTGTAGTTTACTATAGAAAATACACGCGATATAACAATGATAAGGCTAAATATTTTGAAGATAAATTAAAAGAAGAAATCAAAATTGGAGATTTAAAATCTATAGTTTTAAAAAACAAAAATACATTAAATTATCAAGAAGATGTTGCTTCATTAGTTGTTGCTAACGATCTAAAATATCCTGATATTCCTGCAGGCTACAAAGTTGAAGCTTCAAAATACGCTAAACGTTTTACACTATATGGAATGAATGTTTTAGCACAAGATGCTCAACTAGTAAAAATTGACGAAAAGGCTAATGAAACAGCTGAATCAGACAAAAGTATTATTAGTAATAAAGCTAAAAAAATTAAGATTTTTGAATTCTCATTTATGAATAGTCAATTTGACAAAATTTATTTACCATTTAACTTATTTAACAAATTTGAAGTTTTCAAAGCTAAACTTGAACCAATCAAAAGTAACGTTTCTGATTTAGACAAAATCTTCAATATTCATGCAATCAAAAACCCGCTTAACAGTAAACTTTTCGATGCTCTTAAATTATGAAAAAATAATATTAATTTTGCTCATACTTTTATACCTCAAGAATCAGGTGTTAATTACTTTAACTTCAAACTTACAGCAATCTTTACTTTAAAAGAAGAATGCGAAGATGAATTTAATATTCAAACAAATGCAAAATACTGAAACAATCCATTCAAAGTTGAATTACCTGAAACAATTGATGATTCAACAAAATTACCAATGTGATTTATTATCAATAATTTCTACAAAATGGTAGACTATATTGCACTTTCAAAAGCAACAGTTGAAAAATATGTTATGCCAATGATATTCTACTTATTTAATGAAATTAATGACACCAAAGTATTTAATGAAAAAACTCTTTCAGATTTATACGAAATTTAATAGATTAAATAGTGCCCTCAAGGGCATTTTTTAATTTTTGCTAATTTTAATATTAAAAATTATTATTAATATTAGTAAAAAAATATATAATGAATATTATGAACAATTTAATTAGCAAACGTAAGCCAATAATAATATTTACAGGACCTAGCGGAGTTGGCAAAGGAACAATTGAAAAGTACCTTTTTGATTTTAAAAATTTAAAATTACATTTATCATGTTCAGCAACAACTAGAGCTCCTCGTGAAGGTGAAATTAATGGCATTCATTACTTTTTTATAACAAAAGAAGAATTTGAAAAACACATTCAAAATGATGATCTTTTTGAATACAGTTTTCATTTTGATAATTATTATGGAACACTTTATAGTGAAATTGCAAGAATTCATAGAAATGGCGAAATTCCTGTTTTAGAAATTGAAACCAATGGTGCAAAACAAATATTAGAAAAGGTTTCAAATAACAAGAATTACAAATTGATCACACTTTTTATCTTACCTCCTTCAATTGAAGAACTTGAAAGAAGAATTATTAACCGAAAGACAGAAAGTCAAGAAGCTATTAAAAAACGTTTAGCTAAAGCGCAAGCAGAAATTGCAGAAAAAGGAATATTTAGACATCACATAATTAATGATGACCCTTTAAGAGCTGCAAAAGAAATAAAAGAAATTATAGAGAAAGAATACGGTATCAAATAATGGAATATGGAATAACAAGCGACATGGGAAGAGTAAGGTTGGAAAACCAAGACAAAGCTAGTTACTTTACAAAAGGTAACTATGTTTTATTAATTTTATGTGATGGAATGGGTGGTCACTATGGTGGTGGGATTGCTTCAAGGACAACAATAAATACTTTTAATAAAATTTTTAACAATTCAATTCCAGCTGAAAATTCTGAAATTAAAGATTATGTATCTTGATTTAAAAAAACTGTTGATGCTTCACGTGATGAAATGATTAAAATTAGTGCTAATGATGAAGCAAAACTAGACATGGGAACAACAGTTACAGCTGCCTTACTTAATACAAAAACAAAATTATTATTAATATTTAATATTGGCGATTCACGTACTTATGCTATGACAACTTTTGGTGAATTAAAGCAAATTTCAATTGATCACAACTTATACAATAAGTTAATTAATGAAGAAGGTAAAAGTCCTTATGAGGCTAAATTGAATCGTTTCCATGCTTCATTAACTTCAGCTTTAGGTCCTGATAAAAAAACTAAAATTGAAGTTTTTGACTTAAGTAGTCAATACGAACATGTTTATAGTTTATTAGTCACAAGTGATGGTGTACACGACTTTATCGAAAAACCAAAAATGGAAATGATTTTAAGGGAAAATCTTAATGCTGAAGACATGACTAAAAAATTAGTGGAAGAGGCTTTAGATAATAAATCAACAGATAACTGCTCAGCAGGTATGGTCGTATTAGACAATCTAAAAGAATGGAGTTAAAATGGGAATTAAACCACAAAGTAATATATTTAAGAAATATCGTGTGCTATCAACTATTGGTGATGGTGGTTTTAGTCAAGTTTATAAAGTTGAACTTTTAAATGATAAAAGCCCAATTAAAAGAAAGTTTGCACTTAAATATAGTGTTAATAAAAATAAAAATGATGAAGACATAACTCGTAGAAGATTTGAACAAGAAATCACGATATACAAAAAATTAAATACTGAACGTGTGGCATTATACTTTGACTCTTATAGCGATGAATATGAACAATATCTTGTAATGGAATTTGTTGAAGGACAAAATTTAAGAGAAATAATTAAAAGAAATGGTAAATTCACTACAACAGTTGCTGTTAACTATGCAATTCAAATAGCTGAAGGAATTAGTGAATTACATAACTTAGGTGTAATTCACCGTGATATTAAAAGTAATAACATTTTAATTACCAAAGATAAAAATGTAAAAATTATTGACCTTGGCCTAGCTTTAGATGATGAAAGTCAAAGACTTACACAAGAAAACAAAGTAGTCGGTTCTGTTTTCTATATGGCTCCTGAAATTTGTTTAGCTAATTCGAAACCATCTGTCCGTTCGGACATTTATGCTTTAGGCATTTTGTTATTTGAAATGCTAACTGGCTCATATCCTATTTCAGGTAAAGACCAACAAGAAACTTTAAGAATGCAAAGAACAACAAATGTTCCTAACTTATTAAACTTTGTTAATGCACCTCAAGCTTTAGCTAACGTTATCATCAAAGCAACAGCTAAAGATCCAAACAAAAGATATGCAACTATGTGAGATATGCGTCGTGATCTTATGACTTGTCTTGATACTAAAAGAGTTTATGAAAAACCTCTTGATCTTAGAAAAGTTAAACCTAAAAACACAATTCAAGAAAAAGTTAATAGCAAGAAATTTATTATTGCAGGTATTTCAATAATATTAGTTGTTTTGATTATAGCTTTAGTATTGATTGTTGTTTATGTTCACTAATAAGGAGAGAATTTGAAAGGCAAAATTTATTCAATTAATTCAGGTAAATATTACATAAATGATTTGCAAAATAAAATGCACATATTACCTGCAGCTGGTGTTTTTCGTCACAAAGAAATAACACCTTTAGTGGGTGATATTGTTGAGTTTGAAGAAGGCCAATATATCAAAGAAATATATCCTAGAAAAAATTCTTTTGTTCGTCCAAAAGTAGCAAACATCGACAATATTGTTGTGGTTATGTCAATTAAAGAACCTGAATTTCAAAGTTACTTAGTTGACAAATATTTAGCTTTTATAGAAAGTAAAAATATCAAACCAATTATCATTTTGACTAAAGATGATTTAGGTTCAACCCCTTACTTTGAACAATATAAAAATATGGGTTATGAAATTTACCAAATAAACTATAAAATAAATGATTGAGTAAAAATATTTACCAAAATATTTGATCATAAAACTTGCTCTTTAATGGGTCAAAGCGGAGTTGGAAAAACTACAATAATTAATAAATTAACCAACAATAATTATGAAACTCAAAACATTTCAAAATCAGTAAATCGGGGTAAACATACCACAAGAATTGTTCAAATAATTCCAATATTAAATGGTGAATTAATTGACACTCCAGGCTTTAGCTCTTTTGATTTAAACATCTCAAAATTAGAACTTGCTTCTTCTTATTCTCAATTTAGAGAGCTAGGAAAATTATGTAAATTTAAATCATGCTTGCATGAAAATGAACCTGAAGAATATTGCAATATAAAACTCAATGTAAAAAACAAAACAATACCAGAATTTAGATATCAAAATTATCTAAAATTATTAAAGGAGATTAAACATGAATAAAAAAGAAAAATATGTAACACCTTCATTATTAAATGTTGAAAAAGATAAACGTTGTGAAATGGCAACACTATTAGTTAATAATGGAATTAAATGAATTCACTATGATGTTATGGATGGCAAATTTGTACCTAACACAGCTATTGAATTAGATGAAATTAAAAACATTAACAAGAATGCACCTAAACACTTTAAAGATGCTCACTTAATGGTTGAAAATCCTCTTGATTACTTAGATGAATTTAGAGACCATGTTGATATTACAACAATTCATTATGAAGCAGTTAATAAAAAAGAATTGTTGCAATTCTTAAAAGATCATCATTCAGCTTACAGTATAGGACTTGCTATTAAACCAGCAACCGAAGTTAAAGAAATAGTTAAAATACTTCCTTATTTACAACTTGTTTTAGTTATGTCTGTAGAACCTGGCAAGGGAGGACAAAAATTTATTGAACATGCTGTTGATAAAATTAGAGAACTTAAACAATTAAGAGACAAAAATGGTTACAAATATTTAATTCAAGTTGATGGTGGAATTAATGGTGAAACTGGACCAAGATGTTTTGCAGCTGGAGTAGATGCAACAGTAGCTGGAACTTATATTGTTTCAGAACCAACTAAGGAAAGAATTGCTAGTGTATTTGGTAAAAAATAATGGTTGCTTTATTGCAACTTTTTTTATACAAATTAAGCAAAAAAGGAATAAAAAAATATGTTGACTAAAACAACATATAATTTTTATTATTTTGCCTTATTATAGGCTTTTGGTAAATTAGGCAACAACACTAACACCTTTGTTCTTTAAGGTTTTTGCTGTTTTAGCACTAACTCTTAAAGTTACTTTTTTACCATTAACATTCATTGTTACTTTTTGCAAGTTAACGTTAAATTTTCTTTTTGAAGCATTCATTGCGTGTGAACGAATGTTTCCAACCATAGGTCCTTTGCCTGTTAATTGGTCTCTTCTTGACATAAGTATTGCTCCTTTCCTAATTTACGATATTCGTTAAATGTATACAAGTAAGTTAATTATACATTATATGTATTTTTATAAAAATGTTTTTTTAATTATTTTTTAGCCTATTTTTTTCTTGCTTGAGCTCTTCTATATCTTGTAATATTGTCAACAATAATGCAGAAGAATACATAGTAAAGAATTATAAACAATCAGCCAATTACAATTCCAATTCAGAAGAAAATTTCAAAAACAATTGAAAATGCTCTTACTGTTTCTCAACTAATTGTACTTGAGTTATTATTATGAACAATAGCTACCATTACAAAATACAAAATTGCTGTAACTGCTGCAACAGCATAACAAATTAAAGCTACTAAAACAAAAATTTGTGATGCTTTTGTGTGTCTAAAACGACTAAAATCTTCTGTACGTTTTGCCATTATTCTATTGTAAAGCAAGTAAATAATAAGAACAAAGACAAAGGCTATAATTGCTAAGATAAATGCTGCTATTGACATTCCTAAGGATGTAACACCACCTAAAGTAGTGAATACAATTCTTGAAACTCCAAATAATGCCATAGCTATAATAGTCATTAATGAAGCAAGAGCAAACATTACTGTTAATGTTATAAATCTTTCTTTTAAAGTTCTAAACATATTGTCCCCTTTCTAATTTTTTAATGTATCAGAAATGTGTTTTGCAGCTATTGCACCATCAGCAGCTGCTGTAATAATTTGTCTTACTTTTTTATCTCTGATATCACCAATTGCATAGATGCCTTTTACTTTTGTTTCCATATCTTCATTGGTTTTTACAAAACCTTCTTTAGTTGTTATTTTTAGATGCTTAAATGCATCTTGGTTTGGTATAAAGCCTATGAAAACAAATAAGCCATCAGCTACAATATTAATTTCTTTATTGGTTTTTTTATTCCTTATAGTTACTGAAGTTAACTGATTTTCACCATTTAAAGAAATTAATTCATGATCATAATAAATTTTAGCATTTTTTTGCTTTTTGAGTTCCTCAACCAATTCTTCCTCAGCACTAAATGTATTTTGATTTGTAACTATTGTTACATCAGTACATAATTTAGCTAAAAAAGTTCCTTCTTCAACTGCAGAATTTCCGCCGCCTAATACAACTACTTTTCTATTCTTATATAATGCACCATCGCAAATAGCACAAAAGCTTACATATTCTTCAAGTCTTTGATAATTATCAACACCTTTAGGATATCTATTAATCATACCTGAAGCTATAATTAATTTTTTAGCTTGAATAATTTCACCATTTTCAAGAATTGCTTCTTGTAAATTTTCATTAATTGAATTGATTTTTACAACCTTGCCATAAAGATGTTTTGCGCCATATTTTAAGGCATGTTCGTAAAATTTTTGTGATAAGTCAAAACCACTTATATCTTGAATTCCAAGGTAGTTATCTACCTTAGCTGTTTTTTGAATTTTGCCACCAAAGACATCATTATCAATAAAAGCTGTATTTAGTCCTGCTCGAGAGGTATAAAGAGCAGCATTTAGTCCTGCGGGTCCTGATCCAACAATTAAAACATCAAATTTTTTAATATCTTTTTTGTCCATTATTGCACTAACTCTGTTATTGGCTTACTTTTTTAGTTTTAAAAATTCTTGTTTTTTCAATTCATTGGATATATTTTTCAGGATGAGCATAACCATATTGGAATCTGCCCTTTCTTCAAACACGAACATAGTGAGTTGGATTGAAGAATTCAAAGAAGACAAAAGCTAAACCACCTAAACCAATAAATACAATAGAGGTTATTGTATACATGTGGTATTGGTCTTCACGCAATGGTTCCATTGCTAAACGGACAAGACCATATCACACAAAGTATAATGAAGCATTTGAACCTGGTTTTAATAAACCAAATTGGTTAATAATTCATACTAAAACAATGTAACCAACTAAGTTAGCAAGAGATTCATATAAGAAGAGAGGATAACGATAAGCGCCTTTTGAAATATCATCGCTAATAAACATGTTTTGGGCAAAATTTCTTCCTCAAATTTCACAACTTGCTCCAGTAGGATCTATTTTTCCATAAACCTCATGGTTAGCGTAATTACCTCAACGTCCAATAACTTGACCAATTAGAATTGTTGGAATAATAATTGAAGCAACTTTACGAATGTCAACTAGATCTCTTTTGGTATAAACATAAATCATGTCAAGTACAAAGGCCAAGACAACACCACCTTGGATACTTAAACCACCTTCTCAGATTTTGTATCAATTCTTCCAGTTATAGTTTGGATTATAAATTGCTTCTTGGACTAAGTCTCAAAGACGGGCACCAATAATAGCGGAAGGAATTGTAATTAAAATAAGAGTTAAAAGAATTTCTCATTTATATTTTTCTCTTTGTCAAAAAATAACAATTGTCAAAATAGAAGCCAACATACCTAGCATCATAGTTAATGAATAAACTTGAATTGGTCAATTACCAATATGAAATAATGGTGTATGAGTATTCGCTGGAATTGGCGATAATTCATATTTATAATTCATGTTCTTTTTCTCCTCTCTCTTGTATTTGTTTTAAAACATCGATTCCATCTTTTCTTGCTAAGAAAGTACTTACGGCTGCTTCGATTAAGCTAGCAGCACTTGAACCATCTTTAACTGGAATTTCAATCTTAGGTAAAGCTCCATCTAAAATTGGAAACTTAAGATCTGAAAGACCTAAGCGATCTAAATTTTTTGCTTCTTCTGTTTGGTTACTTACTAATTCAACAACTAAATCAATTTCACAATTATCAGTTACTGACTTAACTCCATAAGTATATTTCATATCAATCAAACCAATACCTCTAGCTTCCAAAAAGTTTTTAGTAATAGCAGGACTTTTACCATAATAGGTATTACCAACAAGCTTTACTAAAACAGCATCATCACTAACAAAAATATGACCTCTCTGAATTAATTCTAGGGTTGCTTCACTTTTACCTGAACCACTTGGTCCAACAATTAAAACTCCAACACCACCGATTAAAACTAAACAACCATGAACTTGAACTTCTTCGGAATATTTTTCACTTAAATATGTTCCAATAGAAGCTGAAATGCTTGATGTAGATGAGTTTTCAGTTGAGAAAACCGGTATTCTATATTTGTTGCAAACCTTTAAAATTCATTTTTTAGGTAACTCTCTAACCCCTTTTGAAAGAATAACTAAAGGCGGTTCTTCTTTAAGGACTCGAGCTAAAATGTTGATAGCTTCTTCTTTACCTTTTTTTGTAAAGAAAATTGATTCACTCGTACCTCAACAAATAATATTTCTTGAATAACGAGTGTTTTCAATTTGACCTGATAATTCTAAACCTAATCTTTTAATCGCAGGTTCTTTAACATCTCGGTAAATAGGATCACATTCTTCATTACAAATTGTTAGTTCGAATTCCTTAATAATTTCTTTAATATTTATTTTTCTTTTTCTCATATATTGTCCTTTTTGAAGCTATTAGCTATTTAATATATGCTTTAATATTTTATATTAATTTTAATATATATTAATTATTTTTTAAAAATGTTACATTTTTGTAAATCCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTTTTTAAATTGTCCTTTATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGCGAATGAATTTCTAACAATTATTTTAGAAATAAGGTTCACAAAAATCAAATTAATTTGGATACTTCTAAAGATATTTTGAATCATTTAAGAGCTAGAACTTCAATACAAAATATTTCAAAATTTGTTAATTTTAGTACAATGCTATACGTAAATTTATTGACAACTGTGCTTCGGATTTTAGACATTCCAGAAGAGATAAAAAACAAACTTTTGAGATGTGCAAAGTGTAATGAGTAGGGTCATAAAGTAAAGATTATTTCTTTAGAAAGCAAAATTAAAAATCTCAATTCTTACAGATATAAAAGAAATTGTTTTTGTAGCGAAAATATTATAAATAAATAAAAACATTTATTAAGCAACATAAAACTTTATAAATGAATATAATTGCAAAAAAGAAAGCAATGAAATCAAGAAGAAAAATACTATTTTAAAAATCAATTTAAACAATAATAGATAAAATGAATCTAGATGAAAACTGCAATAAATTTATTAGAAAATATATTCCAAAAGGAAAACATTAGACAATTTATCAAACAAATATATTGCATGAATTCAAAATGAAACTAACTCATTCATAAAACATAAAATTAAAGAATATCCATATGCAATGAGTGCAAATAATTTTTCACCTTAAATTATTATGAATAAACAATACGACCTTCAATGATTTTTTAATTTAAAATTTAAGCAGGCGATTTAGAAAAATGTGTTTTTTTAAACTTACCACCCTCGATTTTGGAAATAATAAAAATGTTACGTTTTAGTTTGAAAAAATAAATACTATTTTGAACTAATTCAACTGTTTTATAGTATTTTAAGTAAAATTTTAAAAATAAAATTAGCAATTTTTTGTTAATCAAATTTTGTATCTGGTTTGACTTTATCCATGATTTCTGGTCATTTAAATTCTGACATGTCTCTTAAATTAAAATTTGTAATTATTTCATCATTTGTTGGAAAAGTTAATGCATTTTTTGAATAAACTTTTTGGATTTTTGCTTTGTTTTTTGGATCAAATATTGTATATAAAAACTTCAATTCTTTTTTATAATTTTTCTTTAAACCTATGACTAAATAGAAACCTCTATTTCATACATAATCAAATAATTCACGATCATTTAAAGGACTATAATATTTGTTATTATCAGGATTCAAATAACTTAAAACTAAGCAATTATATCGTTTGAACTCTTCTATATTTTTCTTAATAAAATCTAAGGTAAAAAAGCCTAAACATGGTTTATCATAATGCTTCATTGACATTCAAAATTCATTACTTGAATCATTTTCTAATTTGACATCATACTTTGGTTTATCACTTGTATCTTCTAAAAATTTAGCTGCTTTTTCACTTGGTTTAATATCAAGAAAAGGATTTTGTTTTGATAAAGCTTCAGCTGTTCCGCTGAAGTTTGCTCCTCCTGATTTATAAAAAGGAGTAATGTTAAATTCTTTTAATTTAGAATCATTATTTAAAGTAATATCAGCTAAAGTCAATTGTTCTTGACCACCAAAAGATTCTAATAATATTCTTCAATTATTTTTGTTAAAATAAACATTTAAATCTTTTGCTTCAACTTCTCCACTTGATAATTGAATCTTTGATTTAGGAAGTTTAAGTACAAAAGCAACATTTTCTTTTGCAACTAAAAAAGTCTTAAGTTGATTTTCTAAATATTCTTTTTTAGCTTGTTTTTTCTTTGACGAATTCAAACCAAAACGTGGATCTTTACTTATTGAAGCAAAATCAGTTTTGCCATGAATTAATTGACTTAGGCCAGTGCCACTGCCTCCTCCTACAATATTCATTTCATATTCATAACCTAATTCTTTAGAAATATCATTTAAATACTGAATCATGCTACTTGAACCCTTGAAACTAATAACTGAAAAGTTACAAGAGGTTGTAAAAGTAACTAAGGGTAGGGAATAAATTGGGGCAAGTGCTCGCAAAAGATTTTTCTTTTTTTTCATGTCATTATTATAAATATTTTTCTTTTTTTATTTTATTTTTTCTTGTTTTTGTTAACATAAAGTTAATTGGTGAGACATGCAAAAAACACTGAAGAATGAAAATAAAAATAAATCTTTAAAATATATTTCATTAGTGATGAGCCTTTTAGCTCTTGCTGTTTTAGTTACCTTTGTTGCTTTTATCTTCTTTAAATCATTATTAAGCTGAAAAGAATTTGGTGTAAAAATCTTTTATTTTGATTATAAAATCGCAGGAGTTAAAGGTGGTTTATATTTCCCATTATTAACTAGTTTGATAATTTGCATATTGGTTTTATTTATTAGTTGTCCTATTTCTTTGAGGGTTGCAATCTTAATAATTTATCGAAATAAAAACTATAAAGCTTATCGAATATTTAAATTTATAGCTTTAATTTTAAGTGCTTTGCCTTCTGTTTTATATGGGCTTTTTGGTTTAGTGCCTTTGACTTGAATGAACAAAAATATATTTAATATGGAAAATGGGACAAACTTATTTAATGCCACAATGGTCTTAAGTTTAATGTGCATTCCAACTATGATCAATTCATTATTATTGCAATTTGAAAAAGTTGATTTATGTCTAAAACTCTCAGGTAGTGCTATTGGTTTAAGTGAATCAAAAGTAATTTATAAAATAATTAAAAAGTCATTAAACAAAAAAATATTTTTAATTTATATTAGCTCTTTTGCCAGAGTTATCGGTGAATCAGCTGCTTTAATGTTTATTTTAAATAGCAATAATTATTCAGAAAATTTTACAAATATTAAGAAATTTTTTAACTCTTCATTGCGTCCTCTTTCAGTATTTATTAATGCTAATTTCTTTGCTGAAAATGGTGGCGATGCAACTAGAAGTTACATGTTTAATTTAGCTTTTGTTCTATTAATTGTGATAACTTTAATTAATGCTGCAATCATTATTATTGTTAATTTAAAAGTCAAAAAACCTAAGTGATTAGACAAGTTTAAAACATGACAAGAAATCAAAGATTTCCTGTTGAAAAAAGATAAAAATTATAAAACTATAAATATTTTATATACTATAAACAAGTGGTTTTGGGAAATTGTTGCTTTAACTTTTGTCTTAATATTTTACTCGTGATTTGCTATTGATCTTTTTTATCGAGTCTTAAATTATTTAGCTTTAGGCAAAATTGATGCTGAAACAAATGTGGGCAACTTTAGAGCTTTATTTAACACTTTATATATCTCTTTAGTAGCAATTTTAATAACCTTCCCTTTTGCTTTTTTAGTAACAATTTATTTTGTTGAATTTAACAAAAGCAAAAAAGCAAAATTAGTATTTGATAACTTCATTTCAGTTATGACTTCAATGCCTGCTTTACTTTTTGGTGTCTTTGGTTCAGCTATCTTTTTAGGAACATTTCAATTGGCTTTAGGTGGTAAAACCAGTAACTCACTTTTAGCTGGAGCTTTAGCAATTAGCGTTATGTTATTTGCCTTCAGCTGTCGCTGAATTAAAGAAGCTCTAGAAATAGTTGATCAAAGTGTAGTAACTAGTGCAGCTGCTTTAGGACTCAGCGAAAGTTCAATTTTATTCAAAATAAAATTACCTAAAATTTGGCCTAATCTTTTAAATATTATTATTTTAATGCTGGCTAAAATCTTTTCAGAAACAACACCATTTATTTTAACTAGCGGACTAAGTAATTCAAAACATATTAGCTTATTATTATTTGGCCAAACATTAACAACTAGAATGAATGCTCAATTATCATTACCTTCTGCTTACTCAATTCCAACTATGTACTACTGTGCTATGATGTCATTTGTGACAATAGTTATCCTCTTTTCATTATCAGAATTTGTGATTCCAAAATTAAGCAAATTAAAAAGAAAACATTAGGAGTAAATATGGAAAAAAATACAATTTTTAAATTAGAAAGTGTTAACTTTTGATACAACAAAAAAGGTAAACAAATTCTAAAAAATATCAATCTAGAATTCAAAGAAAACGAAACAGTTGCTTTAATTGGGCCTAGTGGTTGTGGCAAGAGTACAACTATTAAACTTTTGAATCGAATTCATGGGCCACAAGCTGGAAATTATCTAGAAGGTAAAGTTGAATACTTAAATCAAGATCTCTTTAGCAAAAAATTTATTAATGATATTGAATTAAGAAAGCAAGTCGGAATGATATTTCAACAGCCAAGTGTTTTCCCTCTTTCTATTTATGATAATGTTGCTATTGGACTAAGAAATATTGGAATTAGAGATAAAAAAATATTAGACCAAAAAGTGCAAGAAGCTTTAGAAAGAGCTGCACTTTATGATGAAGTTAAAGATAAAATTAAAGAAAATGCAGAAGCATTAAGCGGTGGTCAAAAGCAAAGATTAGCTATTGCTCGAGCAATAGCGCTAAGTCCAAAAGTACTTTTAATGGATGAACCTACTAGTGCGCTAGATCCAATTGCAACTCAAAAAATAGAGAACTTAATTTTAGATTTAAAAAATGATTACACCATTATTTTAGTAACTCACTCAATGGCTCAAGCTCAGAGAGTTAGTGATAAAACTGCATTCTTTTTAGCAGGTGAATTAATTGAAATTAATAGCACTAGAAACATATTTACTAAACCACTTAAAGAAGAAACAAAAGACTACATTATGGGAAAGATAGGATAGAATTATGCCAATAAATTACACTATATTAAAGAAGAGCGAAGATAACTTAAGAAAAATGTTTTTTGATTACTTAGACCAAGCATATCAAATGCATCTTAAATTAACTGAACTTATTTCAATATATCAAAACAAAATTGATATTGAAGGCTTATTTTTAGAAATCAATAATCGTGAAAAACAAAGCGACTTAACTCAAGTAAATACCATTGAAGAATTGCTTTGAGACATTTCAAAAGACCAACCTTTATTAAGCCACTTGCGTTGATATATTTGTGTTTTAACTTCTTCAGGAGACATTGAGAGAATTTGTGATTACCTCTATTTTATGGCCAGATTCTTCTATCAAAACAAAAAAATTCCTCAAGAGGTTGTTAATGCTGCTTTAGCTATGAATATAGAATTAAGCAAAATTTTTAAAGAATATAGTAGCCGTCTTCAAGATGAAAAAAGTACTAAAGAATTCTTCAAAGAATTCCAAAAACAAAAAGTAGAATACAAACAATTCTGTGATAAAGAGATCAAAAAAATGGCTCGTAAATTAACCAAAAATGATGAGTATTCTAACGAAATAATTATTAAGTTTTCTTTTGAATTGCACTCATATTATCGTGTAGTTGAAAGACTAGAAAATATCATTGAAAATTTGATTTTCATTCATGATACAGATTTCTTTAGAAAGAAATTGGAAAATGAAGAAATGTTTTAAAAGACTCTAGAGTGAGCTTTAAATTCCAGTCTTTTTGGAAAGAAATTCATTCTAATTTAATTTTAAATTTTTACTTATTCCAAAAAAGGAATAAGTTTTTTTATGTAACCATTAACATAGGCAACCAAAATTTTTGTACTCTTTTAATACTGCCTTTTTAATATATTGTAAAATATAAATAATTAAATTAAATAATAGGAGGTTAATTATGTCACTTTTTACAAAATTAGTAAATAAAGCTACTAAAGATGTTTTATTGCAAGCAACTTTTAAAGATGCTAAAAAGATTGAATATTTAATCGAAAAAAACAACGAAATTACAGAATATCTTAGTGAAAACAAAGCTTATGTTTACTTAGGAGAAGAAAGCAAGTTAGATTTTAGAACTCTTGAAAGATTTTTTAGATCTTTAAGTGAAAATAGCGCACGTAACTATTGTGTTGATTTAGCTTCATTTGCAACAAAAAATGTTTCAGTTGCTGATGTTGTTGAAGCTTTTACAAGAGCAGTTTACTTTGTTAAAGCTTCTTTATACAATGAAAAAGCTAAAGATAAAGATGCTAAAGAATTTACTTTAGTTCCTTATGTAGAAAAAGTAACTAAAGAAGTTGAAGAAAGATTAGAAAAAACATTAATTTTAGCTGAAGCTACAAATTATGCTCGTAACTTACAAGTTACACCACCAAACATTTGCAACTCAGAATATTTAGCAGACCAAATTGTTAAAGATTTCAAACAATACAAAAACTTAAAAGTTACTGTTTTAAATAAAAAACAAATTCAAGACCTTAAAATGGGATTATTACTTTCAGTTAACAGAGGGTCAGTTTATGAACCACGTGTAGTTGTTATCGAATATGCTGGTGATCCTTCAAGTAAAGAAAAAACAGTTTTAGTCGGTAAAGGTATTACTTTTGACTCAGGTGGTTACTCACTTAAGAGTCCAAGAAACATGTTAGGTATGAAATTCGACATGTCAGGTAGTGTTATTGTTGCTTCAACATTAAAAGCTATCGCACAACTTAAACCTAAGAAAAATGTTTCAGCAATTATGTGTATTACAGACAACAGAGTTAATGGTGATGCTTCATTACCTGACTCAGTTTGAAAAGCAATGAATGGTAAAAGCGTTGAAATCAACAACACAGATGCTGAGGGTCGTTTAGTTATGGCTGATGGTTTAGTTTATGGAGCAACCAAACTTCATGCAACACGTTTAATTGACGTTGCTACATTAACTGGTGCTATTCTTATTGCTTTAGGTCAAACCTACACAGGTATTTGAGCAACAAGCGAAAAAGCTTGAGAAGATATTTCTAAAGCTGCAGACAATGCGCATGAATTAGTATGAAGAATGCCATTTGATGAAGCATTCTCAAAAGGAATCAAAGCTTCTAAAGTTGCTGATCTTAAAAACACAGACTTAAGCGGTTATGGTGGATCAAGCTCAGCTGCTATGTTCTTAAAAGAATTTACAGAAGGCGTTGAATACATCCACTGTGATGTCGCAGGTACAGCAGACATTAATGAAGTGCCACAAGGTGTTTTAGTCAAAACACTTACAGAGCTTGCTTTAATTTAATTTGAATAATAAAGTCCATATCTATTAAATTACTTATTGGACTTTTTATTTACTAACTTGGAGGAAAAAACAATGTATTTAAAACAAAGTGATGGGATTTTAGAAGTTATAACTGGCCCAATGTTCTCAGGTAAAACAGAAGAGCTTATTAAAAGAATTAAAATTTTAGAAATAGCAGATATTAAAACTTTAGTTCTTAAACCTAAATTTGATACTCGCTTTTCTGAAACAGAAATAGTAAGTAGAACAGGTGCAAAAGTTATAGCTAAAAATATTAAAAAATCAAGTGATATAATGAAATTTTGAGACCCTTCTTATAAAGCTGTTGCTATTGATGAAATTAACTTTTTAGATGAAGGCATTCTTGATGTTATTGATGAATTAGTTTTAAAAGGAGTTAGGGTTCTTTGCTCGGGACTTGATATGGATTTCTTAAGAAGACCTTTTGGTGTAACTCCTGCAATCTTAGCAATAGCAGATCACATTACAAAGCTAAAAGCGGTATGCATGAAATGTAAATCTAGTGCAGGTTTTAGTTTTAGAAAAACAAACGAACAATCATTAAATTCGCTTGGTGATGATACTTATGAAGCTCGTTGTAGAGTCTGCCACATCAAAGGTGAACTAAAGAAAAAAGAAGACAAAAAATAAAACAAATTGCAACTCATATAAAGAGCTGCAATTTTTATATATATCTATAAAACAGTTATTTCGGTAAAATTTCTACTTAAATAAATGTTCAAATTTGTCTACTTTAATAATGGCACTATCTAATTGTTTGTCTAATTTTTTATCAGCGCCTTTAACATATTGAAGTCTAAAATAATCCCTTTTAGCAATTCTTTTAGCCTCTTCTCTATTATAGGCATCTCATTCTTCTTGAGTATATTTTTGTCTTAATAAATATGATTTCATGGCTAAATCTCCTTCTTGCTATATGTATCATTATAAAGGCAAAAAGTAGATTTGAAAAACAGATTTTTTACTTCTAAAAACTCTTTTTTAATATATTAAAAAAGAAAATTTATAAATTTTTTTCAATATGTTTTATAAACTCTTTTAATTTAAAAGCTAAGAAATATGGAATGGTATAAATGTCATTTTGAAAACTTATATTGTTTCTTGAAAGTTTAATACCAAAAGCAACATTTTTATTTCTAAACTCACTTAAAAATTTATTTAAAGAAGAACTATCTCCTTTATTAGCTTTTACTTCAATAGGAATAACATTGTTAAAAGTTTCAACAACAAAATCTACTTTAGCTATACCTTTGTTGTTCTTTCAATAAAATAATTTAAAGTCCTGTTTGTATAAACTATTAGCTATTGCATTTTCATACAAGGCGCCATTATATGTATTGAAGTTTTTATTGATTCTTAAATCATTCATACTTTCAACATCTAATTGTGAAATAAGCATACCTGTGTCAAAGTGATATATTTTAAATGAGTTTTCAATAAAGTTTGTTTTAATTGGCTTTTCAATATTTTTTAATTGATAACAGTAATTAATAACTCCAATATGTTTTAATCAGTCTATAGAAGGTATACAGCGTTTGCTTTTTAAATTCAAATTAAGATCATTAAAAACAAATTTCTTGTTTTCACGTCCTAAGAATTTGCCAACCATATCATAAATTCTTTCAACTTTCATCATTTCTTCATAATCTAAATATTGTCCAAAATCATCTTTATAAATATTTTGTAATTCTTTTTGATATTCAAAAACTTTTCCAAAATTACTTTTTGTATTGGGATTAACTAAAATTTATCAATAATATAAGGAAGGCCACCAATAACTAAAAATTCATCATATGATTAAAAACACTTTGCAGCATATTATTTTCAAAAGGTTTTTGTTCTATCAAATGTTTAAAAATATATTCCCTTATATATTCCTACTATATATTCCCGTCAATATCTGGTATTTAAGATTTTTAGTGAACAAAACAATTCACTTTTCAACTTTATTTTATATCAAAATTTTAAATATGTTAAAAAATGTTAAATTTCTTTGAATTTTCACTCAGTTGACTCTTTTCATGCAATTAGTCCTTGAAAGGCCTTAGATCTTAAGAATGAATGCAAATTCTTCAATTCTAAAGCTTTTGGTTTGTTTAACTTAACTTGAATATAAATCTTCTCATTTCCTTTTGTTGCCACTATATCAATTTTATATATGCCATCCATTTTAAGATTTGTTCTCTTAGCTTGGTAACCTTTAGCTTTTAGAATAGACAATAATTCTTCTTCTCTTTTATAATTAATAAATGAATGATAAAAAAGAACTTGATAATAATTTTTAAATATATCAGGTTGGTGGCTATTAATTTTGGTTAAATATTTACCAAGGGCAAGGCAAAAAACTTTTGATTTAGCTTCTTTTCATTCTTCAAAGCTTTTAGGTTTTAATTTATTTAATTCTCTCTTGATATTAAAATTAAGATCTTTATAGCTTGGCATTTTCATAGTTAAATTATAACGTGAAAAATTTTCATAAATTTATTTGACATTGTTATATATTCCCGATATAATAAGTGTGATAAGTAAAAACCTGCAAACTTTTTACTAATCACTAAATTTATTATTTTCATAATTAATAATAAAGAGTGAGTGGGTTTTGAACTCACTCTTGTTTTTACTTATCATTGTTTGTTCAGTTTGCTTAAAATAAAAAAGCGGTCATTCCCCTTTTATTGCAAACGAACAACCGCAAATTTATTATATATTAAAAAATAAAGAAAGGAAAATAAAAATGTTAATTTCAAGAGAAACATTAAAAAATTGTAGTGACAAAGATTTAAATTATTTGTGAGCTTTGGTTAGTGATATGTCAGACTTACCATTAAGTTATGATATTAACAAATTAATGAGTTGTGTCAATTCTTCTAAACATGGCTGCAGTCATTTAATGACTCATATACAATTTATAGAGTTTTGATATGAAGAAATAAGAAGAAAAATAAAATACTATCTTACTTGAATTAGCAATATGATGGAATTATTTAAATCAAATTTCCTATTATATTTCATAGTAAGAGAAATGAAAATAAGATTAAAAAACATTAAGCTATGTGTTAAGTCTTACAAAGCTAACGAATGAAAATTTGACAATTTAAGAACTCCAGTACAAGTGCAAGTATTTGAAGATTATTTAAATATGGTATATACCGCAATTGATGGCAAATTGAAAGAAAGGGAAAAGGCAAATGATTAAATTTGAAAAGGAACTATTATGTTTGAAAAAATAAAAATGAACAGATGGCGTTGCCGTTGTTGTCAAACATTAACAAATGACAAAAAAAGAGAAGTTTTTAATTACAAAAATGAAGAATATGTGTTCCCTTATTTTTGTCAAAAATGTTGTCAAAGATTAATTAATTGAATAAAAATACATAAATAAAATTAACTAAAATAACTGTTTTATAGTTGTTTTAGAGAAAAAGGAGTAATCATGTTTTACAAAAATAAAATTGTTTTAAATAAAGAAATTGACTATGTTAAAGTTTACTTAGATCAATTTAAAATCTTAGTTGAAAGAAAACAAAAAAGTAAAAAAACTTTTCAACATTATTTAACTTTATTGAAAAGACTTAAAAGATTTGGATCGGTTGAAGAATTACTAGACCAAATGAATCATTATTTAGCTTCTTCAAATTGAGCAGAAAACACTAAATATAGTACAAGATTAGTTTATGCTACTTTTATCAAATGATTATCAGAATATACAGGAGTTTATATCAATACTAAACTAGACTTAGATTCATTTAAAAAAGTTGGTGGAACTAGACAAGCATATAGTATGGAAGAATATTTAAAAATATTCAATTATGTTGAATTATGGGGAGATCTTAAATTCAAATTAATATTCCAAATTTTAGCTATTACAGGAGTTAGAATTGGAGAACTTGCAAATGTTAATTGAACTTTATTAGTTAAAAATAATTTCACTCAAAGAATTGAAACAGAAAAGAAAAATAACACTAGACTTTTCACAATTCCAAAACCTGAAACAAATCATTATTTTGCAAACTTAAGACAAGACATTATTGACAATATTGATTTCTTTAAAAAAGGTTTAAACATTAAAACCATTCAAAATAAATTCAATTTATTTAAACATTATGTTCATGAATTAGAGCCAGAATGAGGCATAAAAATCTCTGCTCACATGTTAAGACACTTCTTTATTGTTAATGCTCATTTAAACATTGGAAGAACAGAAGAAGTTGCAAAGCTTGTTGGTCATGTTAACTCACAAACAACAGCAACAACATATTTAAACTTTAGCAATGTTGCTCTTGAAAATATTATGGAACAAGCTCAAATGGAAAATGAAAGTGCTTTTGACTATTCAAAAATTAAAATTAAATATAAAGAACTAAGCGAAGAAAATGCTCAACTCAAATTAACAATTAGTGAACTAAACAACCAATTAGACAAATACAAAAAAGTTTTAGGTGAAACAAAAGAAAATGTTAATTTTACAGAAGTATTAAATAGCAATATAAGTAAATTAAAAAATTTAATTAAAAATATTAAGTAATAAAAACAAATGGCGACTGCCATTTGCTATGTTACTCACAATTATATTATAAAGGTTTTTTAAATATATAGGCTAACATTTTCATTATTTTTTGGAGCTTCAAAGGTTTCTGCTTTTGGGGCTTCTATTTTATTTTCCTTTATTGCTTCATTAGAAGCCTTATTTGCCTCATTGTTTTTGGGAGTTAATATATTTGCTATGTCTTCAACAATCTTAGCTCTTTCAAGCTTTAATTGCTCTTTAAGCTCACTATTTTCCCTTTTCAATTCAACAATTTCATTTGCATAAGCTCCAGAATGATATTTAGACTCATTTAATTCAGCTTTAACATTATAGACTTGTTCAGTTAATTGTTTCTTTTCATTAACAAGGTTCTCAATTTGTTCTTTTAGCTTTTTAACCAAAGGATCAACATTGCCACTTTGTAAAGCTTTTAATTCTTCACTTAGTCTTTCAATTTCTTTTCTTTGGTTGTCAAACATAACTTCAAGTTTTGAAAGTGAATTGTTTTTATCAGCCAATTCTTTTTGAGTTTGAAGAAGTTCAAATCTTATGTCTTTATTTTCTTGTTTAAGGTTATTATTTTGAAGCTTTAAATTATGAGTTTTAATGAAAGTGGTTGTGATTCCAGTAATAGAAGCTATTAAAGAAACAAAACCACCTAAAAATATAATTAAGTTAGTTGGGTTTGCGAAAAAATCTTTCATTATTTGTTTCCTTTCTTATCATGTTCAATTTTATTGTCTAATGTATTTGTTTTCAAAGCTTTGGAAGCTTCTTTGATTCTTTTAGCTAAATTTCTTTTTTCAATTTTCATTTTTAAAATATAGAGTTTTATTCTAACCTCCAATTTTTATTCTAAATAGGTGTTTTAATCATGAAGCTAATTCTTGACTTATTGCGCTTTCAGGTTTAACAATGGTGAAGACTAAAAAACATATTGAAATAATTAATAATATAAGCAAAATGCTTAATAGTCATAAGAAAAATCTTTTAATCATTAATATGTTCTTTCTCAGCTAAATAAGTCTATTTTTCAAAAACGCAAATCACTAGGTGTTTCATTATTAAAAATTTTGCCAACTTGTATAATTTTAAATTCACAAGACTCATTTAATATTTTAATATTTCATATTGCTAATGCACTACTTGAGCCGATATCATCATAAACCACAGGCTGACAAAAAGTTAATTCATCACCAGAATTTAAATATGTAATAAAGTTATTCTCTTTCCCTGCATAATTAATATAAGTTACTATTTTAAATTTTTTTAGGTCAAATTCATAATTTAGATTAGTCCTTTTTTTATCTAATGAAATTGTGTGCTCTTTGCCATATTCAAATTCTCTTTCTCCAACTAAACCGACTCTTATTCATTTTTTGTCTTTTTCTCTATTTATTTGATTATATAAAGAAATTAAATTTTTATCTATTTCAGCTATTTTTCAAACATTAGATGTGGTATTTTCTAAAGTAGCAACTTTATCTTCTAAAGCTTTTATTTGATTATCTTGTGTGGAATTGATTTGGACATTTTTAGTATTTTGGTTTTCTTGCGCAATTTTAAAGGATTCAAAAGCTTGCTTTAAATTTTCGTAGTTGTCTAAAATGTCTTTTAGTTTTTTAAAACTTTCATTTTGAAATGCATAAAGCACATCGTTATTATTTTTGTATCCGAAACCGTTACCTCATAAATGAAATTTTCCCTCATTGTCTTCAAGTCGTATCTCGCTTTTGCTTTTAATAACATTAGTTTTATATTCTAAATTTTTAATACGCGCTTCTTGATTAATATTATTTTGAGTTTGAGATTTGTCATATTCTTCCAAGCTTTTTATTTTGTTTTCTAATTCAATATCTTTATTGTTAACAAATTCTTTTAATGATTTCAATAAAGCTTCAAAATCAGCTTGAATAAAATCAATGTCACTTTCTAAATCAACTCAACTTGGAAATGTTAATAATTGAATAGATTCATAATTTATTAATTTTGATAATGAACCAAAATCTCTTGTTTCACCATTAAGTGAAATATTATTAGAAACCAGAAAAACACTATTTGAATATTCTGGAAAACTTTTACTTGAAAGTCTATATTTAATTTCCAAATAAATAGCTTTTTTGAAGTTTTCAAATCTTGTTTCTCCAATAATATTTTTAATAGCTTTGGAGTCACTTTGAGTTTTAACAAAATAACTCATTTTTTGACCTATGATTAAATCTATTGTTGTATATATTGAATAAGCAAATTTATTAAAAAAATCTACTTCATTTAAATTTTCATTTTGTTTTTTAAAAACATAAGGAATAGTTAAATTTTGTTCATATCAATATTTTGATTTAAATTCATTCAAATTTAATTCTAATTTGTTTAATTCTTCCATTAATTTAACTCCTTTCCTATTTTTTATATTTATTCTTTACTCAGTGATTAACTCAATAATTAGTCTTTTGAGTTTTACTAGTAAAAGCTTTTTTATTTGCTCACCTTAAGGCGGCTTTTGTTTGATAACCTACATTGCCAGTTGCTCATGATCTAGCCATAGAGCTAATAAATTGAAATCCTGGTGCTCTTCAACCTTTAAAAACATTTTCTTTAACTCCTTTAACAATTTCATTTAAGATTATGTTTTTGTCTTCTAGTCACACTCATTTTTGTCTTTTAAAATTCATAATAAGAGAATTAATTGTTCTAAAAGTAAATAATGAAGTTCCTATTAAATTGGAGAAAATAGGGTGTATTGGAACAGCTTGAAACATTTCAAATTTTCTAATAAATCTTCCTACTATATGGCCATAAGCAATATTATTTAAATAGTATCTTCCAGGACTTTTAGCACAAACTAATTTAATCAATTCATCTATGCTCTTATTTCATAATCTAACAGGTTCTTTTCCTTTAGGATTTTTTTTGCTTTTTGTTGCATTTTTCTTAAAATAGAGAACAGCATTTATAGCTTTAATATCTCAAGGGTTGTTCACTTTATTTATTGACATTCTTTCTAATCAAGAACTATGATCAATTCAAATATAATTAGAAGCTTTCTTAACTACTTTCTTAATTGGTTTTGATTTAAAAGTATTTTTTAATAATTTGGTAAATTTGCCAAGTTGTTTGTTCAAATAGGCTTTTGGGTGTTTAAATACAGTGTCAACTGCTTTTTTTGCTAAATATGAGGGAGAGGTTAATAATGAGACCATAGATCTTAAATGTGTGAAAGCTTTTTGTATATTTTGAACCTTTTTATTCAAAAAATAATAAATATTTTTGTTATCAGATAAAGTTCTCAAAAATGAATCATTATTAAATAATTTTAGTTCTTTTTTTAAAAGTGATTTATTGTCAATAAATTTAGCTTTGTCAACTCCTAATTCAGAATAATTAGCAGCTATTTTTGTTATTTTTTCACTTAATTCTTCTTGACCCTCTTTTTTAAGATTTTTTGCAATTTGAAAAAACTTGTTAGTTTTATGTGTGTTTGCTGCATATCTTATTCCTCTTGTCACAAAAGGAATAGCATTAATTCCAGCGCTAATTCCAAAGTCTTGTCAATTGATATTTCCATAGTTTATAGCAGCATTAAAACCTAAATCTAAGGCAGTACTTAGACCATATTCAATAACAAGACCAAGACCAGGCGCAAAGGTATTAATAAAACTACCTAAAAAACTTGCTCCTATATCCAAAGCAACATGTAGGAATGAAAGAAAACCTCGCAAAAAAGGATTCATTTGTTTTTTTGAATCATTAATAACAAATGATTTTGGGCTTAATTGCAAGGTTGTTTTTGGTTTTGGGTTAGAATAAATTAACATTATTAAGAGCCAGGTTTTGGACTTGGTGCAGTTGTTGGTTTTTTAATAGCTTCAAAGTTTGAATCACAAACTATTCCAAAAAAGTTTTGAATTTCCATAGCCACAAATCTATCACTAAATTTTGGAACTTCGTCAGTTTTAAGAGAAATTAATCTGCTTCCAGAACCAGCAGAATCACTTGTTCAAGCAACAGCCTCAAATTCTTTTAATAATGTTGAAGGTTTGACTTTAATTCCTGCAATCATTTGGAAATCACTTAATAATCATGTTCTTGGGTCTTTAGGATTACCTTGAATTGATTCTTTTACAAAGTTTCCTTGTGTTGAATATCTGACTATTTTTTGATAAATACTTGGTTTGACAAAAATTCCAATTTTGCTTTGTTCAATTCCGTCAATTCCTAATGTTGAATCTTCATGTTCCATTAATCTTAATGCCATGTTTACAATTTCACTATAAACTTCTTCATCTGTTTTTTTAGTCAAATCAATTTCAGTGACAATTGAATCAGGTTGTCCATTTTGTTTTTTTGTTGCATAGTTTTTAATGTGTTCTTCAACTTTTAGTCAATTTTGTTTGTCAACTTTTCTCTTTCATTTTGAAATTTCGTCACTTGCTTTTTGAACAAGCATGTCAGGAACATTCATTAATAAATCAACTTTATTTAATTGAATATCTTGAACATGAGATTCATTTCAACGGATTTCTTTTTTAGCATATCTTGCTTTTTGAGCTCTTGGGTTTTTTGTATATTCTTCTTTAGTTTTTCATTCTTGATCTAAAGAAATTAAATATTCAATAACTGGTGAATTTGGATCACTTGCAGGAGTTAATGTACTACCTAAAAAGTTTGTTTCTAAAATTGCATTCAATAAAGTTGAAATTTTAGTTAGACCACCATTTGATATGGTATAAGCTTTATCAGCTCCTTGTTCAAATAAAGTAGCAGATTCAGCAGTTAAATATTGTTTTAAAGTTGGTATTTGATAAGACATATAATTTCCTTTCTAATAATTTCTAATATAAGGACTTCATGGATCAGAACTATTAGAACTAGAATCTTGGTTAATATTATTTACAGAATTAGGTTCTTTAGTCTTAATAGTTATGTTATTTTCTTTTGCCACTTGCTCAACACTTTGAAACAAATTATTTAGATTAATTGAATTTACATCTATCTTCTCAAAATCAATTTCATTCAATTTGTATTTGTTTCATTCATTTTTAAAAGTATCTTTAACTTTTCCGGACAAGTTTTTATAATTTTCTAAATTGGTGTTGAATTGTTGCTCAAGACTTTGATAATCATTTTTTAACTTTTCATTTTCAGTTTTTAAATTTTGATTATCTTTATGTTGGTTTTGAGTTTTATTTAAGATGAATGAAGTCAAGTCATCTTTTTCTTGGAAAAACTCATAAACTCCAAGCGAAGAAGCAATCTCCTTTTGAGAAGAATTTTCATTTAATGAAAGTTTTTTAAGGAGTTCACTTTCTTCAATTCCTAGTTTGCTAGATAAAACACTAATAATTTTATGCATAGCTTCCTTTCTTCCGTCTTTTGGGCAGCAGTTCCCTTAAATTAAATATAGTTAATTGTTAAGTTACCTAAATTGTGATAATAAGGTAATTCTATTTTTTGGTTATTAATATAAATTTCATTAGGTAAAAATAAACCAAAAATAATTATTCTTTTCTTTTGTTTGCATATAAAGTTTTGTGGAACGAAACCTAAAGAATTTGCAATTTTTAATCATTGATTTTTATTGATAATAAAATCATTGTTTAGAAACTTAAAATAAGTGTCATATATATTTGATTTAAAATCTTCTTTTTCATATACTTGATATTCAATTGTATAAATAGGAACATAAGTTTCATTTATTAATTCATAGTTATCGTCAAGATTTTTGTAATCAAGACCTGAAAAATTAACAACTTCTAACCAATCATTTAAATCATCTAAATCAACAATTTTAAATGCAGCAGGAATTGTGTCATAAGTATAATGAATCATTCCAGGTTCATCACCATGTGCAATTTTAGCTGTATATGTTGGGTTTAATTGAACCTTTGCAAAAGCGTTAACAATGTTTATTTCTTTTTTAACGCTTTTTTTAATTAATTGAACAAACATTTGCTTTTTAGTTAAACCAGGTTCTAAGTCTTTAATTTCTTTTCTAGCTTCTTCTGGTGTCATAGTATATCTAGAAGTTTGAATTGATGATAATTGAAAAGCTTCATTAAATTTTTCTTTATTAAACATTAAAAATTCTTCATGTTTTTGGTCAATAAACATTTTGACTTTATATTTTGAATTATTAAAATCAAAGTCTTTTCAATTATCAGAAATATTGACTTCTTGAATATTTAATGGAATTAGTGGTTCAGATATTTGATAAAGCTTATTTGGACTTTTTATTGCTTTTGGTTTTATGCTTTTTATCAAATTATTTTCATAAACTAAATCATAAAAATTACTTGCTATTTCTCAATTTTTATCAAAATAAAAAGGCAAATAAAGTTCATGTTTTTCAGTTTCACCATTATCAATACAATATGTTGAAGGAACATTGCCTGAACTCATTGCAAATAAAGTTTTTAAAAATTTTTCTTTCTTTGTATATAAGTTAATCAAAGTGTAAAATTTAAAATCAACAAAATCTTTTAAATTGTTTCCAGCAAAAATAGTCTCTTTAAATTTTTTTATTTCTTCTTCAGTCATTCCCATGAAATTATTTTGTAATCTAAGTCTAACATTATCAATTTGATTAATAGCACCTCAAAAGTTTTTCATTAAATCATTTTCTTTGTTCAAAATAATATTCAAATATAAATGAGAAAATTTGCTCAAAGTATTTAAATAGTATTTATTGTCAATACCATTAATTTCAATTGGATTAACAAACATATCAAAAGCTAGGCCATTTGTTGGCAATTCACTAGTTAAACCATTGTTTTCAATTTCTAAAGCTTTTTGGTGCAAATATGGAGTTCTTAAACTGACATTAATTTCATTTGGCTTTTCTTCAATGAAAACAAATTTTATTTTTTTCTCATTTTCAATTTGAGAAATTGGTTGAGCTATTCCATATGAATAAGCATTATTTTTTTGCTCCAAATCATATTGGTTATTTAAAGCATCTAAAATATAAAATTTATTATCTAGTTTCAAAATTGCACTCTGATTATATTTAAAAATAGGTTGTTCGAAATCAATGCTTAACTTTATGACATCATAAATATCTTTTTCAATATCAATTCTATTTTGAGATTCAACAGAACAATATAATTCAATGTTTTTGATTTTGTCTTTAGTTCAAGATATTACATTAACTGAACCATTTTCAAAAGAAAGTGATTTATTGTCTATTAAAATATATTCACCAGTTCTTGGCTCTAAAAAAGATTTTAATTCCATAAAGTCCATTTTATAATTCCTCACTTTCTTCTAAAGCTTTTGAGTTATTATTGATTAAAGGTGTTTGATTTATAATTGAGCCATTTAAATTTGCAGAATATTTTCAAGCTTCATTTTTTAGCCATTCAGTTGAACCAAAAATTAAAACTTTATCAATTAGCAAACTAGGTTCAAAAGTTTTGAAAAAGTTTAATAGTTGCATTTGTCTTAAGTTTGATTTTGCTTCAATATGATCTTCAAAATCACTATTAATTTGTTGAACTTCAGCATTATGCATATTTTTAGTTCCAGAATCACTTGAATCTTCTTTTAAGAAAGCAAATTTTTTAATTTGTTTAATATTGAATCTTATTTTTTCAATTAATGGTATTGCTTGACTATTGCCTTGCACAAGTTGAACAGGAGGAGTTCCATCCATTTGAATAGCAGTATTAATTGGAATAAATCTATCATTAGGATCTTTAAGTCCTTGTTTGATCATGTCTTTAAGACCATTCATATCATTGACATAAATTCAAGGAGCAGATAAGTTTGAATCATTTGCAGCAATTTTTAAGTCTTCATCTAGCATATCAAAATAATATAAATTAACATTAAACAAATCGGGGAAAGCATTATGATTATTAAAGAAAATTTGATAAGGAATTTGGTTATAAAATGGAAGAGTTTCATAGTCATAAAAATCTAATAAAGCTTTAGCTTGTTTTTCTTGTTCAACATTTAATAGAACATTTTTTATAGGATCAAAAAGTAAATCATATTTAAATTCAACTTTATTTTTAATTCTTATTCACTCTTCATCAATATAGAAACTTATATTTGATGAATAAAATTTGATTCTTGTTTTAAGTTTTACCAAGTTACCATTTCTATCAAGCAAATAATCTAAAACTTGAGCAGGCATTCAATTATTTCTTAGAGCAGCAACTCCCTCACAATAAAGATATTGCTCTAATTTTTGCAAAGCTTGCAATTTTGAAATTGTAAAATAGTCTTTAACTTCTACATTATTCTTAAAAATTTTTGGTAAATTGCTTCAAATAGTGTTAATAGTTGTTTGAGTTCCAAATTTTAAAAATTGATTATATGAATCAGGTTTATTATTAAACCTTAAACTAAAATATTTGTTGCTTTCATTTTCTAATTTATTAATAATTGTTTTGCTCATTTCTCTCCTTTAATTCTTTATTAAATAAATAGTTTTTATATTCACAATTTTGTAAGGTGTTGGCCATTTCAAAAGCATTGATGACATCATAAATTTTTTTGTTAATTCTTTCATTTCTTTGGTTAGGATTTTTAGGATCTTCTTTATCAATTACTTGTTGACATAACAGAGTTAATAATGATTTAGCTTTAGGTAATAACTCAACTAAACCATTTTCAAATATGTTTTTTTGTCATACTTGTCTGTCAATTATTCCAAAGCCTTTTTTGCCTTTAAATCTTGAAGCAGGTAACAATTTAGTATTTATCCCATTGTTAATAAAAACTTCAGTCATTCATTGCAAAGAGTGGACATTATCTCCATATAAGAAAGAATCAAAATTGCCTAATAATCATGAGTTATAGAATTTAAAATTGTATTTATCAAAATACTCATTTCATTTTTTGACAATTAAAGCAACTTTATTGTAAATAGCTTTATTTTCTCTTCTCTTGTTTTTTGGTAAGAGCTTTTTAATGTCTTCAAAAATGTCTAATACTATTATTCTTGCTCCACTTAACAACAAAACAACAACAAGAGCACAATTATCAGTTAAACCATAATCAAAACCATAAAAGAGTCCACAAATTTCTTCATTTTCTAATTTTGTTTCTAATTGTTTAATATTGATTATTTTGCATTTTTTTCTATCTCAATTGCCATTTTTGTTGAGCAAAACACTTTTCATAAAATAATCATATCTTTCAGGTGGCTCTTCAAAAGCAAAACCAGCAACAGTAATCATTCATAATTTATAATCTTTAGTTTTTAGAGCTTCAAATTGTTTTAATTGCAAATCACTTAATTCATTTTTAGGAACAAAATATTTAGAAAACATTGTGACAATAATTCCCATTCCCTCTTTAAAAGCTAAGTCTTCTTTTTGCAAATAAGTATTTTCAATTAATTCTTTTGCTAAATTTAAATTGATCTTCTTTAAAAAATCTGTTCCTGAATACAAAGGTAAAATAGGATTATGAAAATCTTTAATTAAAAAGTGATCTACTGTGAAAATGTTATAAAGAAAAGTTACATTAGTTTTAAACCCTTCAATATTTTTAGATCTTAAAACAGTATTAGCTATTAATATTTCTAAATTACTTGCTTGAATTTTATAAATTTCTTCTAATTCTTCAGTTAGCTCTTTTTCAATTGGTTCATCTATTACTAATTCACCCCATGCAAAATCTTTTTGTGAAAGAGTTACACCTTGGAAACTTTGAACATTTTTTCAATTACCAAAAAATATAGCTCTTCCTTTTCCACCATCTTCATAATCTCAATAAGCTTCAGTATTGTAAATACTAATATAAGGTCTATAATCTAAACCTTTAGCAGCAAGAAAACTTAAAACTTTTTCTAATAATGGTTTTAATGTTCCTTTAATGTGGTTATATTGATTTCTACACCAAAAACTATTAAAATTTTTTTCATTCACTAATCTATAAACAGTTTCAATCATTCTACTAAAAGATTTTGAAACACCTTTAGCACCTGAATAGATTTTGAAAAATGCATTAGATTTGTAAGCTTCAAAAGTTGGATTATCTTCTAAATCAGTATAATGAACTAATTCTTTAAATTGTTCTTCTGTAAAAACAGGAGGTTCTTTTAGAAATTTCATTTATCTTTCAAATATGTATAGCCACATAAAATTTTGGTGTGACTAAATCACAAAAATATGTCAGACTTTAAATCATGATCTGCAAATTTATAGATTTGTGATTTAATAGGAACATATAGCCAATCATATTTTTCATTTTTTTTAATTTCTTCAAGAGTTTTTTTGTATAAAGAAATTACTTTTTTGTTTTTTGATTTAGTTCAACCTTTGGAATTACAATCTCAAATGAATTTATCTATGTTGAGTAATAATTTCTCAATTTTATCTTTTTCAGCAATTTCCATTTTTTTAAAAATATCCATTCTTTTACTTTCTAAGTATGTAATGCATTCTTATATTCTTAGAGAGAGATATATATAATGAGTGTAGCTCATATATATCTCTCTAATATAAGTATTTTTATACTTATATAAATAACCCCTATAATCCCCTTTTAATCAAATAAGGAGATCTGATCTTTCTCTAGTGAATTTGAAATATAGTTTTTTGTATATTCTTTACTTAATACAGAAATTTTTTTCAATTGGACAAAAACATTTAAAAATAAATCTTTAATTAATTCTTTTGGAATCATTGAGCGTTGATAATAACTACCTTTTGAATAGTGTTCAGCATTGCCTGGTTTTCTTTTGTTTTTCAAATTTAAATTAATATTTGAATAAAAACAAGTTGGCTTAACACTAAATTCTTTGTTATAGCTTGAGTAATAGGTATTATTTTTAATTCCATTCAAGTTATGGTGAAATTTCAGAAATTTTCAAATAAAAGACTGAAAAGGATTCTCAATTATTCACACTTTAGGTTGAAAGACATTAATTATATAAGCCGTCGCTCCAGCAGTACTTTCACCTAATACTCTTTTTTGTTCTTTAGAGTAAAAATCTCTTTTTTTGTTTTTTGTGGTTTCTACTGTTTTATAAAATTCATAGTTATTCAATTCTCAACTTGTTTTGCTAATATCTTTTCAAATAGCTAGTGAACAGTCTGCTCTACTTCAACTCTCACAAGGTGGAGAAGCAATTATAATGTCAGGTTTTGGAATTAGTTTTAGTTGGTCAATTAAATTAAAATTATTTAATGAAAGGTCAATATTATAATAATTCTTTAAATCTTTATGTTCTTGTATTCCTATAGAATAAATAACACAATTTTCATTGTTTTGTAATGCTTTAAAATAACTCATATTGCCGTCATCATACAAAGCTCATATGGTTATTTTTTTATTTTTATTCATAAACTAAAGTTTTTTTAATAAGTCATCAAAAGCTTTTTCTTGTTCTTCATATTCTTTTTTTATTTTTTTAAGTTTAGATTTTAGAATTTTAACATCTGATTTTAATTTTGTATTTTCTTTTTCTGTTAAATCAATAAGAAATTTAATCAGTTTTTCTTTATTCATTATTTTCTCCCTCACAATTTAAATCAATTTCACTTGCATAACAAGAAACTTGTTCTAAGCATTCTTCTGAACATACTACTAAATTGCCTCAAAAATATCTTTCTTTTTTTGGTTGAAATCTTTTGTGGCAAAAATCACACTTATTATTTAACATAATTATAATTAACTTTCTATAAATTTTGCTAAATGCGGAATAAAATCAATATTTGTTTTGACCAATTCACCATTTCTATTTTTTGCAATTGATAAGTTCAAAATTGTTTTCTTGTTAGGGATTAAAGTGCCAACATTAAAATTATTTGAAGAATTATAAAGAAACATAATTAAATCAGCGTCTTGTTCAATAGAACCAGTTCCTTTTAAATCAGAAAAAGATGGTTCATTATTGGTTTTCTTTTCATACTCTCTTGAAAGTTGAGATAAAACAAAAAAATTACAATTAAATATTTTTGACAATTCTTTTAATTGTTTAGAGCATTGAGACAACTTTTCTCTTTCACTTGAATATTTTTGATTATCAAGCAATTGAAAATAATCAATAAAAACAGCTTTGATCTTTAAACCAATAGAATTCAAACTTTTATAAATAATGTCAGGAGTCACTTCACTTAATTTTTCATTATTAAAATTGTTTCTATATCTATCAAATATAAAAAGGTTCTTTTTGTTTAAATCTTCAAAATCTATTTTCTTAGAATTTGTTTCAACTTCTTTAAGTGAACAAGAATTGGCAAGAGCTAAAAGTTTTGAATATATTTCAATTGAGTCCATTTCAAGACTAAACAAAACAATGCATTCATCTTCTTTCAAATTTTCCAAAGCATTAAAAATAATGTTAAGAGCAAAAGAAGTTTTTCCAACGCCTGGTCTTGCAGCTAAAAAATAAAGTTGTTTAGGTTTTAAACCATTTGCATAATTATCAATGATATTGAAGTTAGTTAATAGAGATTTATTTTTTGCAATATTTTCCATTTCAATTTTGTAAAATTCCTGTAACTGTTTTGTAGTGAGTAGCATATATTTTTCCTTTCTAAAATAACAAGGGCATTAAAAACTCCGGTGGGTCTTTTTTTTCAAAGGGGGAGGTATATATAGTCCTGAAATTTTAATTTTGGGAAGTATATCACTATATATTCCCTGTTTCTTTAGGAAAATTCGGTGTATAGGTTAAAGCATAGGAAAAAATTATTGATTTGATTTCCTTAGTTGGTGTGGTGGTTTGATATAAATGTATAATACATTTATATAAACCACATACAATATGAAGTAAGATAATAAATAATTTTTAAGTTAAGTTTAAATATAAAAGAATTTTCCTAAAATAAAAGTATTTTGAAAAGTAAACTCATTCACCTATTTGAACAAAAAAACCTGGCTAATACCAGGTCTTGACTATTTATTAGAATACTATTTAGGTGTTATATAAAAAGATAATTAATATCTTCATCTTTATATCCTTTTGCTCAAAGGTATTCTTCAAAATCCATAGGTTTCATTTCATACTAAGAAATATAACCGACTCCAATGTGTCTAATATTGTGTTCTTTAAAATTAATTCCTAATAAAAAACCTGAGCAAATAACATCAAATCTTTTGTCTAAGGCAAAACTTTTTAAACTTGTAATGCAATTAGGTTGTTCTTGAATTTCATCAAAAAATATTAAAGTTTCATTTGGCACAAATTCAAAACCGCTTTTTGCAAAAGAAATAAATTTAATTAAATCATCAACTTCATAAGATTTAACATTTTTGTTATTAAACAATTCTTTTATTTCATTCATTAAAGCAAAGTTGATATAAACTACATATTTGTAATTATTTTTAGCAAATTCTAAAATAGAAGTTGTCTTGCCTATTTGTCTTGCTCCTTTAATTATTAATGGTTTTTTATTGGCATCATTTTTTCAATCCAACAAAAAAGCATCTATTTTTCTTTTTAACATAGGACCTACTTAATAATAATTTTAATTATATTTTACTTTTTTCCGTACTTTTTAAACAAAATCTTTACTTTTTCTTAAAAAAGAGCATAAAAAATCCTTAGATTTTGCATCTAAGAATCTTAAATTTAATTAACTTTTTAGTTTTGTATCATAGTAAACTTTGATTTCAGCCATTGTTTGTTTACTTTCTGGTTCAATAGGAAAATGTTTAACTTCATATTTCATTTTTGGTGTTGTATTATCAACATCAAAAATAGTTTGTTCTTTATTTATTCCATAAAGACTAGCAATATATTGTGGATATTTATCATCAACAAAATAGTTATTTTTAACAAATTCATATTGTGATTTTCAAGCAGGAGTATAACCTACAAAGTCAAATGTTGTAAATGATTCATAACCTTCTATATTATTTCATTCTTCAGTAGTACTATTTCATTTTGGACCATTGATTCATTTATCTTTTTCAACTCCACCAAGAAAGCTCTTGTGAACTGTTTTATAAATTAAATCAGTAAAATAATCACTTGTATTTTCTGAGATAACTAACCCATCAACTAATAATAAATTAACTTTTGGTCTTAAGAATCTAATAGTACTTTGAGGCACTGTGGCATAGTTATCTTCTGAGAAATAAGCATCAACTGCATCACCGTTATAAATTAAACCAGCACATGTTTTCGAATCAGGATTAATTAAGTTATTTAAAAGAAATTGTCCATCTCCATTAAAGTTAATATGTTTTGTATCTCTAACACTATAACCTATACCATGATTAAATAATTCAACAAAGTGGTCAATGTGTTCTTTATATCAATTAGGCTTATCTTTTGATGTGCCTTTTCCAGTAGCGTGATCATCAACAAAGCCTTTATTAACGTCATAAACATAAGCTGAACCATAAATCATATTATCTCTTACAGCATTTGTTGCTTCTCAATGTTCATATTTGTTAGCTTTTAATGTTTTAAGAATATCAATGAGTCTAAATTCACCTTTGTTGTTTTTTATTTCATTAATTAGGTGATTTTGCATTGCTTGTTGATGAGCATTGATTTTATTTTGATATTCAGGGTCATCTTTTTTAAGATTTAAAACTTTAGCAGGATTATAAGCTACTACCATATCTTGTGAAAAGTATGGAGCAAAGTAGTCAATTAAGTGACGATCCTCAGTAGCTTGCGAACCATCGGCATTAATACATTTATCTTTAGTTAAATATTTGTTATATGACTCAAGATGATTTTTTACTGTGTCAGTTAAAAGTTTTAAAACTTCATTGCGGTCATTAAATTTAGGATCACCAAACATTTTAGCATAATCAATTTTGCGAAGTTTACCCTCTCTAATAAGTTGAACTGCTTGAGAATCGTTGCCAATTCCGGCAGCTGCTTTATTAGTTAAAATAGCTTTAGTAAAATCTTGAATTTCAGCAAATTCTTTGAAGTTGAAGTTTTTATTTATTTTTGCTTTGGTATTTGGATCAATATAAGATTGATAGTTGTAGAATGAAGGCTTGAAGTTATTTTTGTTACTATATTTATAAGATAAAGCACCAACTAAAGCAGCTGCAGCAACAACTGCAAGAGCACTTGTACCAAAAGTTTTGGTTAAAATTTTGGTTTTAATAAGTGTTTTAAATTTGTTGTTCTTTGGTTTGTTGTTGTTCATTTTTTATCCTTTTTGCTTTTTTATTACTTCTTTTAATCATGTAAGCTTTATAACCAATATAGCAAGCATTAGCAACAATAGTAACAAATAATAAGCAAGCTCCAACTACTAAGCCTCAAGCTTTAAATTGACCTTCATAAAGTTTGGTTCCAAGTGTACTTACATTTGAAGTTGTTTGTAAAATAATAAAGTCATCAAATGATAAGAAAATGGAAACTAAACCAGCAAAAACAATTGATGGAATCATGTAAACAAAATAAGTTTTGAATCATGATCTAATTTTTGAATAACCTAAATCTTGGCTCGCTTCAAATAATGAAGCATTGAATTTTTCACTTCTTGGATACATTAAAGTAATACCATAAGGTAAAGCCATAATTGTGTGACCTACTATTCCTCTAATCATTCCTTCTTTAGTGTTAGCTAAAGTTCCGAAAAGTGCTGTGAATACTAAAACTAAACCAATAGCAGTAATGTTATCTGGGTTAATAAAGGGTATATTATTAACAGCTCTAACTGCTCTTGAGTAGTTTTTATTTTTTTGTTTTCACATTGCAAACACTGTCACAAGTGAAATACTAATAACTAATAAAGATACAAAGAAAGCAATAATAATTGAGTTAATTAAAGCTAGTTCTCTACCTTCATCAAAAAAGTTTGTTCAAGCAGTATTAGTAAAACCATTTCATGAAAAGCTCAAGAAACCTTTATCACTTGGCTTATTAAAACTAAAGATAACTGCGAACACTAAAGGAATATAAGTTACAGCTAAAATAATGTAAATGTAACTTCTTTTAATAAAGTTTCAAAATTTAGTCATGTTTCTTACCTCCTTTAGTTTTTTTTAAGTCTAATTATAATTTTAGGAACAAAATTGATTAATCCGTAACAACCTATAAATATGGCTGATACAACTAAAACAAGTGTGCTTCCTAAAGCTAATTCATATTTGTTTGAAGGGCTTGAATAATCATTAATTACAGATCCAATAGTTTGTAATTGAGTACCATTTGGAAGTAATTTTGCACTAATAACAAAGGTGGTTGCTGATGATAAGAAGATCATACTGAAACCACTTAAAATAGCTTTGAAACTATATGGAACAACTACTTTAAATAATGTTTGAAATTTGTTGTAACCTAAGTCTGCACTAGCTTCAAGAATGTTTTTAGGCATGTCTTTAAAGACTGTGTAAAGTGGCATAATCATGTATGGCAAGTTTAAATAAGTTAAACCAAAAACCATAAATCATTCAGCATTCAAACTGTCTTCTTCAAAAATTGAAAGGAAGAAACCTCTAATTGCATAAATTTTAGCAATTGTAAAAATTATCATAGGACTAAGAATTAAACTTAAAGCATAAATTGCTAAATATTTGCTCTTAGTTGTAGCAATAAAATAAGCATATGGAAAAGCAATAATTAAACATAATATTGCTGAAATTAATCCAATTTTTAAACTACGTCAAATTATTAATCAAATTCCTTTTTCAGCTAATAAAGCTGATGCATTAAAGCCATCTTGTGGTTTTAAAGCATTAATTACGATTAATAAAATTGGCAAAATTATTAAAAAGATAGCAATAAATAAATAAGGTAATAAAAGTAGTAATCTTTTATTTAAACCAAGTTTGTTTTTAATTTTTGAATTCATTTGTTTTGTAATCCCATTTGCTATCTTTATTCATTAAATGAATTGAGTCAATTGTTCAGCTTAAGTTTACTTTTTCACCTATTTCGAATTTTTTAGCTGTTTCAACATAAATGATTTCATCATTGTCAAGTTGAATTTTTAAGTAGTAATAACTTCCACGGTAAGCCATGTTAATAATTTCACCAACAAGCTTGTCTTTTTTCTTTTCATCATAAGCCATGATATCGATGTCTTCTGGTCTAATTAAAACATCAACTTCAGTTTGGTTTGGAAATTCATCTTCATCATGAATGGTTTTAAATTTACGTTCTCACATTGTAACGCTGCCATCTTCATTAAAGATTCCATTGTAAATATTTGAGTCTCCAACGAATGATGCAACTCATTTATTAACTGGATAGTCATAAATATTTTTTGGCGTGTCATATTGTTCAATTTTTCCACCACGCATAATTGCAATTCTATCTGAAAGTTCAAGAGCTTCATCTTGATCATGTGTAACAAAAATAAATGTAAGACCTAATTTTTGTTGTAAGCTTCTTAATAATATTTGCATTTTTTGTCTAATTTTGGCATCAAGAGCACTAAGAGGTTCATCTAATAAAAGAATAGCAGGTTCAATAACAAGAGATCTGGCTAAAGCAACACGTTGTTTCATACCACCCGAAAGTTCATTAATAGATCTTTTTTCATTACCTTGAAGTCCGACTAATTCTACAATTTCTTTAACTTCTTGGTCCATTTCTTCTTTTGTTATTCTTCTAGTTAAATAACGTTTTTCAAAATTTTCAGTTTGTTGTTCAACATAGTTTTCTCAATATGAGTAATTGAAGTCTGATTTATCTAATCATTTTTGTCTACGACGATATGCAAAAGTATTTGGTTTTAATGTTTCCATTTCTCTTTCATATTTTTCTTGTAGAGCATCTAATTTTTTCATTTCAGCTTGAGCTTTTTCTTCTCATTGTTTTTGTTTTTGAACTAATAATTTTTCATGTTTAGCATTAATAACTTCTTTTGGTTTTCTTTTAAGAGCTAAACCATATTTAATGTTTCCTTCAACATCTAAGTGAGGAAATAATGCATAATCTTGAAAAATAGTTGAAAGGTCTCTTTTGTGAGGCGGCAAGTCTTTAATATCTACACCATGAAATTTAATTTCACCACGTGTTGCTCTTTCAAAACCAGCAATAAGTCTAAGGATTGTAGTTTTGCCAGATCCTGAAGGTCCTAGCAAAGTAACAAATTCACCTTTATTGATTTTTAAATCAACATTGTCCAAAACAACCTTATCATCGAATTCTTTAACTACTTCTTTTAATTCAATAATATAAGGTGTATTATTTTTCCTAATCATAAATTCTCCTAAAAAATAAAATATAAATTTTTTCTAAATAATCTGTTTTATAGCGTTTTTGGTAATAAATAAATTTAAAATATTTTATTTTGGCGTCAAATCAAAGTTAACTAAATTTAATTAAATCGATTGACTTCTTAGTGAGGCTAAATCAAACACGTTTTGAGAATACTCAATAACAAAAAATGGAAGATCTGGATTTTTATCTTCGCAAAAGTTTAATAATACAAATTTAGATTTAAATTTATCACGCTTAAAATTTTGTATTCTCATAATGAGAATAATTATAATAATAAAAAATATTTTTGTTTAAAAATAATAAAAATTTTCATTCTTTGTTTTCTGCATTTTTTATTCATTATTTTCTTTTGTTTTTTTAAAACTAAATAGCATTTCTATTATTACGTTTTTAAGTCTTTAATGATGTTATTTTATTTTTTATAAAAATCTAAGTAAGGAGAAATTATGAGCATTAAAAATAAGAGAGAATATGGTTTGGTTTCATTATTCTTAATACAATTATTTTATTATTAGAATTATCCTTAATAATATTGATTGCAATAAAAGGAAAAATAGGCTTTATGTTATACACCATTTTTGCAATATCTATACCATATGTTGTTTTAGGTTTTTTATATAATGATTTAATACAGGAGTAACATTGTTTATACAATTAATTACAATTGCTTTAGTTATATTTTTAGCATTAAATACAGCAAGTGGGCCAATTTCAAAAGATAGTTCTCATAAGAAAAACAACTCAAATTTTATTTTCACTATTGCCTACTTACTTTTGTCTTTTAATTTTTATATTAGTTAGAGTATTAATATAATTGTCAAAACCTGTGAAATCAAAAATTAATTATTTCAAAAGAAATGATTTTGAAGCAAATGATACAGAATTTGAATCAACTTTTATTGATTTATCAGATAAAAAATAATAACCTAATAATTTTTGTTAATTCCAAAAGTAAGATCTCTAAAAGTTAACTGTTTCACTTTTGGAATTAGGCAACTTAACATCTTTAAAAATAATGTTTTGCATTAATTATATTTAATGTATAATAAACATGATTTTTTCTGTTAGTTATAACTAACAAATTTATGAAAAAAGAGGAGGCTATTATGGCAAATATAAAATCAAAAGTAAAAAGCATTGCTAAGATGGAAGAATTTCGTGTACGTAACAATGCTATGAAAACAAGAGTTCGTAAAGCAATTAGAGTAGCTAGAGAAGCAATTTTAGCTAAAGATAAAAACGCTCAAGAGTTAGTTAATAAAGCTCACTCAATTATTGCTACAGCTGTTCAAAAAGGTGTTTTCCACCCAAATAAAGGTGCAAGAAAATCATCTCGTTTAGACAAATTTGTTAACGAACAAAATGCTAAAAAAGATGCATAATTAGCTTCTGAACTATAAATTAAAAAACCAGAAACTTCTGGTTTTTTGTTTGCTTTAATCATTAAGCTTTATATTTAAGTCCAAGTGCTTTAAGCATTGGCATTAAGAAGAAATTAAATGGTTTGTTAAAGTGAGGCAAGAAGAAGACGTCAGTTAAAGCACATTCAGGAAGAGTCAATTTTCTTTGAAGTGCTAAACCAAACATGTAAATAACTTCTGTATGATTTACTTGACCTCATGATCCGATTTGAACACCTAAAATTTGTAATGTTTTCTTGTCAAAAATAATTTTGCATCCAACTTTTTCACTTTTACCAGCAAATTCGCAGCGGTCATTGTCTTCAAAATATTCACTAGCTACATCAAATCCATGAGCTCTAGCTGCATCTTCATTAAATCCTGTTGCAGCATAGTGACAACCAAAAACATTAATAGCATTTGTTCCTACTACTCCTGGAAATGGCACTTTAAGTCCAGCTAAATTTAATGCAGCTACAATACCTGTTTTAACTGCATTAGTTGCTAAAGCTACGTGTTCATATGTGTTGCTTACACAGTGTTTTAATGCTGCAGAATCACCTAAAACATAGATGTTTTCGTCAGAAATTGATTGTTGGAATTCATTAACTTTAATTGCTCCATTAGGTAATTTTTCAACATCATTCAATACATATGTTCTAGGTTTGAAACCTACTGACATAATTACAAGATCTGCATCATATTTACTTTTATCTGTAATAACTTGTGATACATGTTTTCCATCTTTTGATTTGAATTCAACAACTTTTTCTCCAAGAGCAAGTTTAACACCTTCTTTTTTCATGTTTTTTTGCATGATGTCTGTAAATTCACCATCTAAGTATCCCGGAACAACACGATCAGCTAAGTCAATTAAAGTAACTTTTTTGCCTTTATTGTGAAATGCTTCTACTAATTCAATTCCAATATATCCAGCACCGACAACTACAACATTTTGAATCTTTTTATCATTTGCTTTTTTAACTAAAACTTGAGCATGTTGATATAGTTTTGATAAAAGAATGTTGTCTAATTCAATGCCTGGAATCTTTGGTTCAATAGGTCATGTACCACCTGCAAAAACCAATTTATCATATGTGTCTTTAAAGGTTTTACCACTATCTAAATCTTGAACTGTAACTTCTTTTTTCTTTCTATCAATTTCGATAACTTCATGTGAAGTTTTTAAATTTACTCCATATTCTTTTTGCAAAATATTTGGTGAAGAATAAAATAATCCATCTGGTTGTTTAGCTTTTCCACCTACTCAAATTGCAATACCACAACCTAAAAATGATGTGTTTGTATTACGGTCATAAGCTGTAATTTCTGCTTTAGGATTTACAGTTTTTAATGTTCTTAAAAAGCTGGTCCCTGCATGGTTTGCACCAATGACAATAATTTTCATATTTCCTCTTTTCTGTAATAAAAATTTGTATTTGTACATTTTTATTTTATTTAAAAATGTTTTGTTTTAAGTGTAATATGAAAAAAATATTTTTTTACCATAGTTTTATTATTAATAAAAAAACTTGCATTTTTTGCAAGTTAATTTGTATTAAATTTAATGATTATTAAAAGATTTGAGCAACATTTGTTAAGCTAAGTAACACTAATATATCAAGAATTAAGATAATTAACAACACTGCTGCAGCAATACCAACACCTACTCAAATTCCTTTGTCTGATTTTCGTTGAACAGGGTAGAATTGAGTACCATCATACATACCTGTTGGGTATCCATAGTTAGCTTCATATGGAGTTAATTGCCCATAGGCTTCAGGTTGTACTAAATATCCGCCTTCTGTTCCATAAGGATCTGCCATAATTGTTTCACTTTGTACATATGGATTTCCATAAGGGTCCATTGCTGGCTGTGCATTTAGATCAATTGGGTTTCCATATGGATCGTATTGAACTTCAGCATTTGGATCATAAGCTGGTTGTTCTGCTGTTGCAGCTGCTTCTGCTTTAACTTCTTCAATTTTCTTTTCAGCTTCTTCACGTACTGCGTTGATTTCTTGATCTTTTTGGTTTTTAATAGCTTCAATTTCTTCTTCAACTTTCATGAAGTCGAATTTTTTGTTTTCTAATTGTTCTTTTAAGCTATTGATTGTTTCATCTGAAGCATTTTTTTGGTCTTCAAGTTCTTTAATTTTAAGTTCAAGAGATGCTTTGATAGCGTTAAGGTCAACATATGATGATTGACTACGTTTTTTCATTTCAAGATCTGCTGGGAAGTATGTAGCAGGATCTGAAATTAATACAAAGTCAATTTCTGGAATTCTTTTAGCTAAAGCCGATTTGTAAGGTTCTCTTGAAAGATATGATGGGTGAATACTAAATTCTTCACAAATACCGTTATATGTTTCACCACCATCAAAACCTGAAACTTTAGGAATTGATAATAATTTTTTAGAATCTTTGTCTTTTGGATTTTCTGATTCTAATACTGCTAATTGTCCACCAAAAATACCTTCGTTATTTTGAAATCAAATGTAAACTTCATCTCCAAAGTTCAAGAATCAGTCTACAGCATCTTGACGTGTTTTAAATTTTGCAAGCCCTTCTTTAATTTTAGGGTGTTTAAGAAGTCATGGATATGTTTCATCTTCTTTAGGTTTTCATAAAAGATTAAGTCTTTTCATAATTTATTTCCTCGCTTGTCCTAATAATCTATATATTAAGTATTTGAATTATAATTAATTATTTAAATTTTGTACAAATTTTTATAAAAAAAATAGTAAATTCAGCATATTTTGACACTTTTTGCACTAAAAAAGCACTTTCTTTATTGAAAATGCCTTTAATTTTTAAAATCTATTCCAAGTAAATTTAGTCCATTATCAAATACATTTTTGACAGCTTCTAAAATTGGATAGAAGTTTCTATAATCTTTTTTAAGTTTATCTTTTAGATTTTGATTTATCAAATAACACATTTCTTTTAGATATTTTGTTAAGTAAAAAGGATTTAAAGTTTTGATTACTTTTCTTAAAACATAAGGAAAATAATCAAGCCTTAAAATAAGTTTGCTTAAAAAATTGTTTTGATTCATTTTGTTACAATAACATTTATTGTTTTTTGATTCATCTAATAATTTATTTATTTTATTATAAGTATTTAATATGAAATCGAAATTATGACTAATTTTCTTTTGATTTAAAATTTCCTTTTCAATTGTTATTTGATGTTTAGTACTCTTTTCTAAAAAGAAAAATTTGATTTCATTTAAATTAACTTTTTTTACTAATTCATCTATAAAAATGACGTTTTGGTTTTTTTCTGTCATACTTTTAACTTCATTATTTTCTTTAATTTGGAAGTTTTCACAAAAGGCAATATCAATATTATTATTGTAAGCATTTAAGAATTTTAAAGCAGAAATTAAAGAGTGTGAATATTGAGTATGGTTAGCATCTAAAATGTCAATAATTCAGTCTGCTCTTTTTAATTTTTCAAGATGATAAATTATGTCTAATAATAAATATGAACTCGTGCCATTTCTATGAACAAGAATGCGTTCTTTACTATCATCAAGATTTGTATTAAGTATTAAATTTGAATCATTATCATAAATAAAAGGTGACAATAATTCTAATACTTTGTCAATCTTTTCTTTGACCAAAATGTCTTCAAAATAGCTCACTATGTCAAAAAAGACACCTAAATTTTTAAGTTGTTTTTTGGATTTATTTAAGAAATAAAAAACAATTGCCAAATCAAATTTATTTCTTTTAATTGTGTCATTTATGTCTAAATAAAGATCACCATTTTGTGCTTTAATATTCTTTGCACATTCTAAAATATCCTTGTCAACTTTAATAGAATTGTCATTTAATTCAAACAAGTTAACATATTGTTCATGAACAATTTGGCTTACTTTTTCCATTTTCTTTAAACCTGGATAAACTAAAAATTCTCGAGTAACTTGATAACCTAAGTATTCTAAAATTCTTGCTATAGTTTCTCCAACTAAAGCATTTCTAGCATTCATCAAAGTTAAAGAATTAAGTGGCGAAGCTGAAGTATATTCAATATTAACTTTAATATTATTTTTCTTTTCTTGTCCATATTTATAAGCTTTCTTAATAACTTCATTAACCACTGAAACTAAATCTTTTTCATTCAAAACAATATGTAAAAGCCCATAACGATCAATTGTCACATCTTTAACCATTGAATCTTTGATTAATCTCTTTTTAATTTCTTCTGCTAATTCAATATAAGGAATAAAACTAAATTTTTTAAGTTGAAAAACAATATCGCATTCAATATGGTTAATATTATTGTTATGGCCCTTAGTTTTACGAGCCATTTTTAGTGAAAACTTCTTTTCACCATCAAGAAACTTAAAAGAACTGAAAATCTTGTTTTCAGCTTTTAGTTCATTTACTATGTTTTTAATTAACTTGTTCAATTTATGAACTATAACCATATTGCTCCCTGAATATATCTTTAAATATATTTATTTGTATTTAATATTTTAACTTATTTATTATAATTTAACAAATGTTAAAAATTTATGTCTGCGGTCCAACTGTTTATAATGATCTACATATTGGAAATATGGTTCCAATATTAACTTTTGATTTAATGCTAAAAGCTAGACGAGTTTTAGGAGAAAAATTCACTTTTGTTCACAATATTACTGATATTGATGACAAAATAATCAATCGTGCTAGAGAGCTAAATATAAGCGAAAAAGAATTAAGTCAAAAATATACTAAAGACTACTTGAAATTATTAAAAACCTTGGATGTTGACACTATAAGTCATCTTGAAAAAGTTACTGACAATATGGGCTTAATTATTGAATATGTTAATAAGCAAGTTAAAAATAATAATGCTTATATTGACCAAGAAGGTAATGTTTGATTTGATGTTAAAAAGTTTGAAAATCATTATGGTCAAGTAGCAAATTTAAATCTTGACAAAATGATTTTTGAAGAGCAAAATAATAACAAAAAATTTGAAGCAGATTTTGCGCTTTGAAAGAAAACTAATGTTGGAATTCAATTTGATTCACCTTTTTCAAAAGGTCGCCCAGGCTGACATACTGAATGTGCGGTTTTGATTAATAAACACTTTAAAAACAAAGGTTTAGATATTCATGGAGGCGGAATGGATTTAACTTTTCCTCATCATGAAAATGAAAATATTCAACACTTTGCTTTATATAATCAAGACATAACTAAAGAATGAATTCGTTGTGGACAAATTAATTATGAGGGCGAAAAAATGTCAAAATCTTTAGGCAATGTTATTCTTGCAAAAGACTTTTTAAAAGAACATGATCGCGAAATTTTGAAGTTGATTATTTTGAGTTCAAAAATTTCAGCTCCAATTAATGTGACAAATGAATTAATTGAAAATATGTATGTTATTTTGAATAAATACAAAAAATTAATTTTTAAATTCTTTATTAATTTTAAAAATATTGACAAATCAAAAATTAATAAAAATGAAGAATTTATTAAGATAATGAATTCTTTAAAAATAGGCGATTTTTCTGATTTTAATTATTTATTAAATGAACAAATTAAAGAATTCAATAAAACCAAAAATATAAAAAATGCAGAAATAATTTTTAATACTTTAAAAGTTATCCACCCTCAATTAACAATTAAAGCTAACTATAACAAAGCTTTCGAGCTTTTTGACCAATGACGCTGTTTTATAGACAAAAAAGAATATAAACAAGCTGATAAAATTAGAAAAAAATTAATGAAATTAGGAGTTTATTAACATTTTTTGAATTACTATTTTTTAGGAAAAATTGAGGTGCTAAATATGGAAAAATTATATGTGTGTGGTAGAAATTCAGTTGAAGATGCTTTAAAAGTAAACTTGCCAATTGAAACAATTTATGTCAACTCATTTCAACTCGCACAAAAATTAAAACAAATTACCAAAGCAAAAATAGAAATGAAGGATAATTCATTTTTTGCTAACTATCGTAATGATAACCATCAAGGTGTCATTGCTATTTTAAGAGACTTCCCTATTTATGATCTTGAAACAATTAAAAAAGATAATCCTGAAAATGTTTTAGTTTTAGACCACATTCAAGACCCTCATAATTTAGGTGCAATCATTAGAACTGCTAATACTTGCGGAATTAAGCATCTTATTATTAGTAAAGAAAGATCAGCTAATATTACTTCAACAGTTTTAAAAGTTAGCTCAGGTGGTTTTGTTAATATGAAAATAATTAAAGTTAATAGTATTAGTGCTACCTTAACAAAACTTAAAAAATGAAACTATTGAATTTATGTTTCAGCTTTAGATGAAACTGCTCAAAAAGTAAATCAAGTTACTTTTAATAAACCAAGTGTTTTAGTAGTTGGCAATGAAGGAGATGGTGTTAGCAAATCAACTCTAAATGAAGCTGACCAAGTGATTTACATTCCTCAAAAAGGAAGTGTTCAATCAATGAATGTTTCAGTTGCAACTGGTATTTTGTTATATGAATTAACAAAGGACTAACATGAAATATTTACAAAAATTTTTAGACTTACATCAAACACCCACGCAAGAGTTTGATGAATTTTTACATAGCTTAAAAGACAATCAATTGATTATGATATTGGATTATTTTTACAAAAATGAATTTATCAAAAATATCAAAAGCACTTTAATTAGATTTCCTTATATTCCATTAGAAGCTGAAGATATTTATATTGAATTTTTGCAAACCTATTTAGAAGAAGTTAAAAAGTATAATTCAACTGACAAAAATGTTAAATTTTTAAATTTCTTTTTAAATATCTCCAAATTTTACACATTAAATAAAATTAGATATTGGCTTAGAAAAAAAAGAATTCATAACTCACTTATGACTTCAACTGATGAATTGTTATATGTTTTAGATGAGCATTCTGAAGAACAAATTGAACAAAGAATTAATCAAATTGATACTGAAAACTTTTATCATTTATTAACTGACAAAGATAAAAATATAATTAAAATATTGCAAAATAGTATCAATCAAAAGGACAAATTGATTACACCTAGTAAACTAAAAGAATTTAAAACTAAATTTTTAACTAAATTTAATAATTATTTTCATTTTGCCCATTAAAAATGTTTATTAAATATTAATAAAAGAAGCAAAAATAAGTTATAAAATAAAATTTGCTTTTTTTTAATTATTCTTTATAATTTAGATTATGCAAAAAAGAAAAGTGACTCTTTCTTGTGAAAATTGCCAAAAACTTAACTATTCAACAAATAAAAGTTTAAGCAATCCTAAAAGAATAGAGATTAAAAAATTTTGTCCAAACTGTAGAATGCACACAATACACAAAGAGGAAAAATAATGAAAAAGGAAAAAGAAGTCAAAACTAAAAAACCTAAAAAGTATCTATTCAGACGTTTTGTAAAAGAAATGAAAAGAGTTAGATGACCTTCTGTAAGAAAGAATTGAATTTCTTTTGCTCAAATAGTTATTTTTACATTGATATTTACATTGTGTGCTGTTTTACTTGGCGTTCTTTTCTCACTTATTCTTACTAAAGCAGGAGTTAAATAATTGGAGGATATAATGGATAAGAACTTTCAATGATATATGATTAGTACAGTTTCTGGAAAAGAAGATAAGGTTGTTGAAGCTTTAAAAAATAGAATTATTAGTGAACAAGTTGAAGATTGCTTTAATCATGAAGCAACACCTGAAGGCGCTTTTAAAATTTTCAAAAAACCAACCTTAACACCTAAAGAATTAGAAAAACAAAAAAATGGTGAAGAATACAAAGTTAAATATGTAAACATGTATTCAGGCTACATTTTTATTAATATGGACATGACAGATAGAGCTTGATTTATTGTACGTAATACTCAATATGTAACAGGTTTAATTGGATCATCAGGCCGTGGAGCAAAACCTACTCCAGTTTCACAACACCAAATTGATAAAAGTTTTAAAAAAGAAATCAAATTACAAGAAGACTTTGACAAAGGCGTTACACATACAAAATTCCAAATTGGTGAAATTGTTGAAATTATTGATGGACCTTTCAAGGGTGAACAAGGTAGAGTTTTAGAATGTTCAGAAGCAAATAAAAAAGTAACTGTAGAAATCGAATATTTAGGTAAAAAAACTCCAGCTGATTTTGAATACAAAGAAATTAAAAGTCAAGAATATGAATAATTTTTTAACTAGCAAAGAGCTAGTTATTTTTGACTTATTTAACTATAAAACAATTTAATTGATACAAAAGAGCATAATAAAAAAGCCTAATTAATAGACTTTATTTTAACTTTACATTTTGTTAATTTTAGTTCATGAATGTGGTCAATCATCAGGAATTTTTCCTTTGTTAATAACCACCACGCTAGACGTTTCATTTAAAACATTATCATATTCAAAGCCGCCCATTTCGCCCATGTATTTAGCAATATTTACAATATCAGTAAGCTCATCTCTAAAGTTTTCGCCTTTTAATAAAACAAATTTTCCTCTGTTTTTTAATAAGTGCGCTGCACTTAAATATAAAACTTTAATGCTTGCAACAGCTCGAGCAGTAATAATCTCAAAGTAATTTGTTAATTTAAAAAACTCTTCAACTCTTGAATTAATTATATTGACACTTAAATTAAATCGTGCTTTAACTTTTTCTAAAAAATTGCATCTTTTCTTTTGAGATTCAATAATAGTTAAATTGAACTTATTATCTAATGCAATTAGAATTGGAATTGAAGGAAATCCTGCACCGGCACCTACATCTAATATTTTTTTGTTTTCTAGATTTTCATCATAATGCATCATGTAATTTTCAAAAGCTAAAATAGAATCAAGTATACCTTGTTCAAAAATATCATCTAGTGTTTTAAAACCTGTGATATTCATAACTTGATTTTGATCATAAATCATTTGAGCATATTCTTTTAATATGCTAATTTTTGAAGAATATTTTTCAAGTAAATCTTTTTTAGTAATTTTTCTATAGCTCATTATTTATTTGAAGTAATTTCTTTTGCAAATTGTTGATATAAATGAGAAATAGATTTACCACTAATTGATGATTCAATAACATTAGCTAGGAATCTGTCAAGACTTATTACTTCGAGTTTTTTGAATTTACCATCTCTTAATTCTGCATTTTCAATTGAATCAGTAATAATTACTTTATAAACATTTGGATTATTTTCAAAAATTTCAAATCCTTTTGTAAAAATACCGTGAGTTGCAGCTACAATAATTTTCTTTGCACCGTGTGCTTTTAATGTATCAACTGCTTTTAAAATTGTTCCTCCAGTATCAATGATATCATCAATGATAACTGCATTTTGACCTTCAATATCACCAATAAGTCCTAAAACTTCAGTTTGGTTTGTGCCAATACGACGTTTGTCAATAATACAAATTTTTACATCGTTTGAAATTAATTCAGCAAGTTTTCTTGCTCTAATTGTTCCACCGTGGTCAGGTGAAACAACAGTAAATGATTCATGTGTATTTTTAATAGCTTTTGCTAAAGTATAAGCACCACGCAAATCATCAATAGGAATATTGAAGAAACCTTGAATTGCAGGGTTATGTAAATCAACTGCAATAACTTTGTCAGCACCCGCTGTTTGTAATAAGTCTGCTACTAGTTTTGCACCAATAGGTTGACGTCCACTTGCTTTGCGATCTTGACGTGCATATCCATAGTAACTTAAAGCAACTGTAACACTTTTAACACTTGCTCTTTTAAGCGAGTCAATAAATAAGAATAACTCCATTAAGTTTTCATTAACTGGTCTTGATGTTGAAGCAATAACGAAAACATCTTTTTCTCTAACTGTTTTGTCGCTAACTAACATAACTTCGCCATCAGCAAAAGTTGTTTTAGTAATTCCTGTCAAAGGAACTTTAATAATTTTTGAAATTTTTTCTGCTAATTTCAAGCTATTCGGCATACCAAAAAGCATTACATTTTTTTTATCCATTATATAAATTCCTCATTAAAATGACTTTTATATTTATATTTTAAACTAAAATTAATTAAAATGAATCTTATGGTAAATTATTGTTGAAAATATCATTTTTAAAAGTTTAAATTTTATTTATAAACCTATAAAAAAGGCTTTTAGCTTTATTTTTAGACTGATTATTAGCTATAAATAAATATGCTTTTTAAAAATGAATATTTTTATATAATTATAAACATGGAAAAAAATATGTTCAATTCGTTGTCTGATATTAAAAATACCTATGACAATTTAGTTAAAAAATTGGATGATCATGAAGTTATCAATAACATCAAGGAATACACAAAAATAAGTAAAGAAGTCAACAAAATTAAGGATATAGCAGAAACCTTTACTAAGTACTTAAATTTACAAAATGACTACAATGATGCAAAAGTAATGATCAATTCAAAAAATGAAGAGGAAGTTCTTTTTGCTAAAAGTATCATTGAAGAAAATGAAGTTAAATTAGCACAACTTGAAGAAGAATTAAAAATTTTAATTCTGCCTAAAGATGAAAACGATGACAAGAATGTTATTATGGAAATTAGAGGTGCAGCAGGCGGTGACGAAGCTAATATTTTTGCTGGCGACTTATTCAAAATGTACACAAAATTTGCAGAAGATTTAAATTTCAAATTAAAAGTTATATCTTCTTCAGCAGCTAGTGTTGGTGGTTTTACTCAAATAGTATTTAGTATTAAGGGTGATAAAGCTTATTCAAAACTTAAGTTTGAAAGTGGAGTGCATCGTGTGCAACGCGTACCTGTAACTGAAAGTGCAGGTAGAATTCATACTTCAACAGCAACAGTTACTGTTATGCCTGAAATTGATGATAGTGTTGATATTGAAATCAAACCACAAGATTTACAAATTGATACATATAGATCATCTGGAGCTGGTGGCCAATCAGTTAATACAACAGACTCAGCGGTTAGAATTACACACTTGCCTACTGGTATAGTTGTTTCATCGCAAGATGAAAGAAGTCAAATAGCAAATAGAGAAACTGCTTTAACAGTTCTTAAAAGTAAGCTTTATGATTTAGAAATTCAAAAGAAACAACAAGAAGAAGCAGGGTATAGAAAACTAGCAGGTCATGGTGATCGGAGCGAAAAAATTAGAACATATAACTATCCACAAGATAGAGTTACAGACCATAGAATTGGATATTCAACAAGTTTAAAACAAGTAATGGAAGGTAAATTATTAACTATTATTGAAGCATTACTAACAGAAGAACAAAATCAAAAAATAAAGGAAAGTGGCTTATAAAATGAATAACGAAGAAACATTAATTTTAGAAAAAAAGCGGTATGGTTTGGAACCTAAAATTACTGAAAAAGAATATGAATTATTAAGGCAAAATTATCCAATTCAATTAATAATGGGCTATGTTGAATACTTAAATACTAGAATTAATTTAAGAGAACAAGTTTTAATTCCAAGATATGAAACTGAAGAAATGGTTGATATTTATCTAAAAGAATTTGCAGCTGAAAATCAAGAAGTTTTAGATCTTTGTTGTGGTTCAGGTTTTATAGGTTTAGCAATTAAGAAAAACTTACGTTCAGCTAAAGTAACTTTAGTAGATATTAGTGATGAAGCTATTAAACAAACCATTGAAAATTCTATAGTAAATTTTGGTTTTAATCATGACTTAAAAATTTACCAAAGTGACTTATTTACTAATGTTAAGGGAAGATTTGATGTTATCATTTCAAATCCACCATATTTAAATTGCAATGACAAAAGTTTAGACTGAAAAGCATTAAGTTTTGAACCTAAAATTGCATTATGTGCACCTGAAGATGGCTGATATTTTTATGATAAAATCTTAAAAAAATACAAGAATTATTTAAAGAAAAACGGTTGATTAATTATGGAAATTAATCCTCAACACATTGAAAAATGAAAACAAATTAAGGGAGCACAAATTAAAAAAGATATCAATGGTAAATATCGCTTTGTATTTATTCAAAATATTTAGCTTTTTTAATTTATAATAAGAACATGGAAAAAAATAAAATTAAAGAAATTGTTATTGCAAGATTAGACAAACTTTCAAAGAAAAAGGTAACTTTTACTTCAACACTCGAAGAATTAAAAATTGATTCATTATCACTTGCTGAATTAATTTACGAAAGTGAAATAGAATTCAAAATTAGAATTGAAGATGAAGTGCTTTTAAAAATTAAAAATGTTAATGATATTATTGATGCAATAGAAAATGCTAAGAAAATTTAATTTTTGTGTATAATTATTTTTAGCTTATTAATAGGAGTATAGTTCAATGGTAGAACAACGGGCTTCAACCCCGTGTGTTGTGGGTTCGAGTCCTGCTACTCCTGCCATATCAGAATGACCTTTTTGCAACATTTGTTGCTTTTTTTATACAAAAAATCTAACCAAATGGTTAGACTTTTTTGATTATTATTTATTTTGGAATGATATTACTAATTTTTGATTTTTTCCTTTTATACGGAAAATAACTTCATATTCATCATACGTTTTTCTTACTGCATCAATTAAAACTAAATTATCAAGCACTTGTTGTTTAAGATGAGGATTACTTTCAATTTCAACATTTTTAAATTTTATAAATGATTTTATTTTAACTATTAATTCATTTAATTTTTCATGAGAATCAATTGAATAAAAATCTGTTTTATCTTTAATTAATGCAAATAATTCTTTTTGTTGAGCAACTGTAAAATTTTCAATTTCAGCTCAAATACCTTTAAGTTGTGAAGCTACTGGGCTTAGAAACTTATCTATATCAAAATCTAAATTAATTCCATCGGTTACATAAGGTAAACTATATCCATACATTTCTCTCATTTTGGTATCTATTACTGATATTTGAAGATTATTTAAACTATGACTAATAATTCTAACTTTAAATCTGTTGCCTTTATCTGTCGTTTTTGTATAATCAATTTCAAATACTTTTTCAAGATCTCTATCATGTAAATTTTCATCATAATACTTATTATCTTTTAATTTAATGCAATTATTTTTTATAATTTCATCAATAAATTTATTTAACTCATTGTTTGCAAAAACTTTAATTACATTTAAAACTTTCTTGTCAAATTTATTGCTATCAATATTAAATACATGTGTTTCATATCCAAATACCAATTTAACAACAACTTTGTTATTTACTTGATCATATGTAAATTCTACTTTGTCAAATCGATTAAGTCTTTCATAAACTTTAACATTTTCGAAAATTAACATCTTGAATAAATAAGCATTAATAAAGGAAATAGCTGAATCATGTTCTTTTATTTTAGCACCACCAACAGACATTAATTTTTCAAGGAATTCTAGTTTGTCTGCTTTAGATGACATAGCTTGTTCAACACCATATGCACTCATTAAGTTAATAAATTGTGTTTGAATTTCTTGTGAAATACCAATTGTTGCATCTTTTAATTCAGTTGATTTTGTGCTTAAACTTGGACAATGATTTTCTCTAATTTCTGAATCATGTTGATAAACAAATTCAAATTCATCTACAACTTTTCAAGCCTTAGCTTCTATTTTGAATATTTCAACTTGAATTTTAATTTTAGATTGATAGGCATCAATATTTGAGAATTCTTTAATTGTATATCTAAACAATTTTTGATCATCTTTAATCGCTTTGTTCTTTCTAGCTATTTCTGCTTGTTTGAAATATGAATTTAAAAATTTATTAAATTTAGAATCAAATTTATTATTTAAATAATTTTCAATATTTGTAACAATGATCTTTTTTGATTCTTCATCTAACAATATTGAATCATATTCTTTTTGTAGTTCTAAATCAGAAAGTACAAATTTGCTTATATCTTTTTTATTATCAGAAGTTTCTTTTTTAATGAAATCAAGTAAATAAAAGTTTTCTTTAATATTTGCTTCTAAATTGTGTTTAAAATCGTCTGTTTTAACATATTCTTCAAGTTTCGAATCTGTGAATGGAAAACTATTATTAATTTTTGGATCAAAGGTTTTTGGCGAATTAATTGAATTATGTGTTAATTTAATTGCTTCCAAGTCAAAATGATTATAGTCTGGTTCATATAATTCTAAATCTTCAAAAATTATTCTTGTATTTAAATTAAAATTATTTGATGAATCATAATATGTTTCAAATTTACAATTAGGAGCTAATTTAGCATAATTGCCTAAACTTCCACCAGAATCATAAGCATTAATATCTCATTCATTATCATAATAACTTTGAATTTCATTTAAAGCACTTTGATCTGTAAAATCTATTGCTTTAAATTTATTTGATACTGTATAAAAAAGATTACTACCATTCATTCAAGATTTTAATTGACGTCCTAATGATTCATTAAATGTATCAAGACTTTCATTTGGATTAATATAAGCTACCATTGACTTATTCAATCTGTCTACTAATTTAATAAGTGGATTTAAATCTTTAAAGTTATTAAATTGCAAGGTTCTAACTTTACTTATAACTCTTTGATTTTTATTGCTTCCATCTTTTTGATAACTAAATGTTACATCCGCTTCAAATGAATTAACATCTTTTTGACGATAATCAATAATATTTAAATAAACGTTTTCACTTTTTAAATCTGGTCATTCTTTTTTTAATTCAGCAATTGCATTTTTTCATTTTTTTTGTTCAAAAAATCCTATTTGTTTTTGTTTTAATTGAGAAAGAACTTTATGGGCATGATATTGTTGTAAGCCTTCTGGAAGTGAAAAGTCATAATTATCAATTAGTTTGTCCAAATATGCTTTTTCTGTTGTTTTTTCTGTAGCTAAAGTAATTTCTTTTGTGAATTCTTTTGTTGATATTTCATAAAATGACTTTTTACTTTTATCATCTAAATTACGACAAAGTTTAAATTTTAATTCTACTTTATCACCATTAATTGTGTAATCAGTTACAACAAATTTAGTTTTATTATATATAAAAGCTCGTTCCAATATGTTTCAACTATTAAGTGTTGTAATTGGATTAGATAAATCTTTTTTTGCTAATGATTCTAGTGCTGATTTTGTAAGATCATTAATGAGTCAAGAACTATATTTTCCATAAACTTGTGTTTTAAATTCTCCTGTGTAAATATCGAAGTTTTCTGGTTTTAGATAAAACTCTTTTGCTTTGTCAAAAATTGCATCTAATTCTTTAGATATTCTAAATGTTGGTCTTTTTTGTGTAGTGGGCATTGTTGCAAATACTCCGCTTGGTGTAACAGATAAATTTTGAGATTTTTTATTGTATAAAGCAGGTTTAAAACCATCAATAACAGTAACTTTTTTTATTAAACTATTTGTATTTTTATTTCAAAAACTTCAACTAATAATCACAGTTGAAACATTATAAATATTTAAAGTTCAAGATTCTAGGCTTAAATCATAAATAGATGAATCATAATTTTCAAATTTAACTAAATTCGCATTATATTCATAAATAGAAAAGCTACCTTTATCTTCATACGCAACATTTACAGCTTTTGCTGCATTAGCTACATCTATTAAATCAGGATTTTTAACACTATCAAATGGTTTGAAACCTTGAACTTTACAATAACCGTCAACAGAAATATTATTTGCTTTTTCTCTTAATTTAAACGATATATATAAATCGCCTGTTGCAGTATCCACTAGTTTGTTGTCGTAGTTGATATTTATTTCAAATAAGTCCTCATTAAAACCTGTAGCAACAAAATCACTAGCTTCAGTCTTATCTACTAAAACATCTTTTTTAAGAGCTGGATTATAATAATCAATTTTAACTTTTTTTACTTCTTTAAGTAAACTTTCTGAAGGATTATTAGCTGGAACTTTCCCTGGGTTTGAAGTAGTAGCAGATTTTTTGAATCCAGTTATTTCATAGGTATTTTCGTCACTTACAAGATTTGCAGAATTCTTAATTTTAAAGTAAATTTTTATTGAAGTTTCTTGCGCTTCAATTCTTCAATGTATTAATTCATATTTATTTAAATCTAAATTTGATACAACTAAATTTGATATGTTTTTAATATCCTTTGCAAATTTATCTTGTTTATTTTTAACATCAAAAACAATATTTTTTGCAAGTTTATTTAAATCAGCTTTTGTTTCTTCACTAGTTGGTTTACCGGTTGTTCCACCATTATCTTTACCAACACAACTACCTGCTACAAAAGCAAAAGGTAATGTACTTAATGAAGATAGGATTAAGAATAATTTTCTTTTTTTATTTTTCATGATTTCCTCCATTTTTTCTAAAAATCATTTAACTAACTAATTATTAGTTAACTAAATTATAATGTATTTTTGCAAAAAGTTTAAATTTTGTTTAAAAAATAAGTTTTTTTTGCACTTTTTGAATAAATCATTCTTTTAAAGCTTTTTAAAATTTCACTCATTTTTTGGCTCTTTAAGTAATTAATTTCACCTTATAAAAAAATAAGCATATTTTTTAAGAAAAAGTTAAAATTCTTGTTTTAAAATAATCATATATAAACAAAATAAGGAGTTATTATGTCAACACACATCAGCGCAAAAAAAGGTGAAATAGCAAGAATTTGTTTTCTTTCAGGAGACCCTTTAAGATCAAAATTTATGGCTCATAAATATTTAAAAAATGTAAAAGAAGTAAGCTCAGTTAGAAATGAAAATTACTACACAGGAGAATATAGAGGTATCAAAGTTACTTTTGGAGGACATGGAATGGGTATGTTCTCAATTGGAGCTTATGCTCATGAACTTTATAATGAATATGGAGTAGATGTTATTATTCGTTTAGGATCAACTGGTTCATATTATGACGAACACAATGTTATGGATACAGTAATTATTAATAGAGCTTTTAGCGACAACATTTCAATAGCTCAATTAATAAATAAAGAAAATACATATGAATACTTTCCAGACAAAGGTGTAACAGATGAATTAATTAAGGCAGCTAAAAAATTAAACAAAAAAGCACCAGTAGTTTCAGTACACTCAACAGATGTTTTCTATGCAACTAGACCTTTAGAGGAAACTAAGAAAGTATCAAAATGTCAAGTAGTTGAAGCAGAAAGTTATGCGCTTTTTGCAGAAGCTAAAAGAAGCAATAAAAAAGCCGCAACCATTTTACAAGTTAGTGACTCTTTAGCTAAAATGGAATTTAGTGATTATAAAACTAGAGAAACAGGATTTACAGAAATGTTCGAAATAGCTTTGGAAGCATTAAAAAATCTATAATGATTTTTACTAAAAAAACTATAAAAAAGCCGCATAGCGCGGTTTTTAATTTTTGATTTTTTTAGTGAATTGAACTCCAAAAGCTATTGAATATAGCAATAGTGAAGTGCCTGCTACAATTATGAAATTAACATATCATTGCATTTGATAATCGTTAAATTTGTAAAAACAACCCATTATTTTTTTAAATTGTTCTATGTGTGTTGGATCACTAATTAGACGATCAATAGCAGGTGAAACTAAGGTTTGTCTAAATAAAACATTAGCATGCGAAGCAGGAAAGCATTTAATAATTCATTGAAGCGCATCAGGCAGTGATTCCATTGTTGAATAACCACCAATTATAAAACCTGCTATAGATCCAATTAAAGCATTGATAGCTGCAAAAACATTATGTTTTTTAATGAAGTAAACAATTAAAAAGCTAAAACTACTTCCTATTAAACTTGAAACTAAAATCAATCCAATTACTTCAACAATTTGTAAAAATGATAAGAATTTTGCTCCAAAGCATAATGCATACACAAAACTAATAATAAATGATACAGTTGACATAATTACAGTAACAGCATAAATAGAAATAGCGTTTCCAATAATTATTGAAGATTTATTAACTGGCGAAGCATAAATGTCTTTATAGGCTTTTGATTCTATATCTTCAACCATGAAACTTGAAGCACCTAAACTAACTGTAATACAACCAATTGTCGTTGTAGTTGAAATCATTCATAAATTCATTATTTCTCTAGCGCTTTTGCTTTTATCTTCAATCACTCATCCTTTAAGTCCATCACTAATTGATTTACCTAAAAAAACTGCAACTATAAAGAAGACTAAAATAACACTTAATAAAGAAAAGAAAACATTAGTTTTGTCCATGAAATATTTTTTAATATTTCTTTTAATTATTGCACCCATATTATCTAATCTCCTTTCCAGTTATATTGATAAATACATCATCCATATCACCCTTTAAAACTTCGAAAGAAGATATCAAATCTTTAAGTTGATTTGCCAAATCTATAGCTTTAAACGAAGAGTCTAAATATATATCAATATAATCTTTAAATTCTTTGAATTTTAAACCTTTGTTTTTTAACAATTCAACTGTTTTAAAGTACTTTACATCATTTTCAAGATATACTTTTAACCTATCTTTTGTATATTCTTCTTTTAGTTTTGCAGGAGTATTCTTAGCCACAATTTTTCCATTATCCATAATCACAACATAGTCAGCTTTTTCAGCTTCTTCCATATAGTGAGTAGTTAAAAATATTGTTGTTTTATTTTTATTTTTTAATTCATAAATTAATTCTCAAATAAGCTTTCTAGTTTGAGGATCTAATCCTGTTGTTGGTTCATCTAAAAATAAAATTTTTGGATAATTTAATAAGGCTCTAGCTATATCCACACGACGTCTTTGACCACCTGAAAGTTTTTCATATTTTCTTTTCAAAATATCTTCAATTTGAGTATCTTTTACAACCCTTTCAAAAGCTGCTTTTATATCTGCTTTTTTACGATAATAATATGCTACTCTAATTGTTAAATTATCTCTAACACTTAATTTTCGATCTAATACACTATCTTGAAAAACCACACCAATTTGTTTTTTGATTTCATCACTTTGTTTCTTTAAATCAAAACCACAAATTGTTATTTGGCCTTCATTATAATCATTCAATGTACACAAGGTATCAATTGTGGTACTTTTGCCTGCACCATTAGGCCCTAAAAATGCAAAAAGCTCTCCTTCTTTTACAATGAAAGAAATACCTTTGACAGCTTCAACTTTGCCATATGATTTTTTTAAATTTTCAACTTTTATTATTTCAGACATAAAAACCTCCTTAAAGTTATAAAGCATATTAATTATAAAAAAAATTTTTATAGTGTGGCATGGTTTTTACATTTTAGAAAACTTTTTTGCGTTTATTTTTAAGGCTTTTAATTTTTAATATATGATTATAAATATATAAGTAATTTAATGTTAATATTAATTATTTGGAAAGGGGTATTATGGCATATAAAGCACTTTATCGAAAATATCGTCCGAAGTCTTTTGATGATGTTAAGGGTCAAGATCATATTGTGCAAACATTAAAAAATATCATTTTAAATCACAAGATAAGTCATGCATACTTATTTAGTGGACCAAGAGGTGTTGGTAAAACTTCAGTTGCTAAAATTTTTGCTAATGTAGTTAACTGTGGTCATGGTGAAGATTTAACTAAACTTTGTGAAAGTTGTATTGAAAAAGCGGATCAAAATTTTGATGTTATCGAAATGGATGCTGCTTCAAATAATGGTGTTAATGAAATTAGAGATTTAAGAGATAAAATCCAACATTCACCAGCAAGTGGAAACTACAAAGTTTATATAATCGATGAAGTTCACATGCTTTCAAAAGGAGCTTTTAATGCACTTTTAAAAACATTAGAAGAACCACCAGCACATGCAATATTTATTTTAGCAACAACTGATCCTCAAAAAATACCTCTAACAATTCTTTCAAGAGTCCAAAGATATAATTTTAGGAAAATATCAAACCAAGTTCTAATTAACCAAATTAAAGAGGTCTTAGATAAAGAAAAAATTAAATATGAAGATTCAACCATAGCTTATATTGCAAGATTAGCAACTGGTGGTATGCGTGATGCACTTTCAATAGTCGACCAAGCTGCAGCTTATGGTAACGACAAAATATCGCTCAAAGATGTTATGTATGCTTTTGGAATTACTTCAAATGAAAGCTTAATTCAAATTGTAAACTACCTTTATCAAGGCAAAATCAAGGAAGTCTTAATTTTATTTAATAACCTTAAAAATGCGGGAATTGATTCAAATCAATTTGTTCAAAGCTTAATAGAATTGTTCAAGGATTACTTGATTTATGCAAAATCAAATGATGCTAATTTATTAGAACTTTTATCTCTTGAAGAATTAAATACTTTGAAAATTGATGTTAATTTTGCATTCAATTCATCTGAAAAACTTTATAAATTATTCAAAGAATTAATTTTTTCAGAAAACCCATTCCAATTAATCGAACTTTATTTAATTAAACTTAATCAAACTAATGAATTACAAAATAGTAAGGAGCAAACTATGTCAATTGATGTAAACAAAAAGAAACCTAAAAAAGATGACACAATCAAAACAGTTTTAAATCAAACTCAAGAATTAGTACTTGAAGCTAATCGTGACGCTGATGACAATTTTAGCCTAGAACAAGATCTTCTCAGTAGCAATTTTGAAATAGAAGAAGATTATGATGATGGTTTAGTAAGTACAGCAGAAATTCAACTAGATGATGAAATTCAAAATATGCCAAAACCAGTAGTAATCCCTGCTTTTGACTCAAAATACAAAAACTTGAAAACATTCAAATCAAACTTAAGTAAAAAAGATTTAATCAACTTACTTAAACAATCAACATTAACATTTTTAAATAGATACAAATCAAATTTAGATATTGTGATTAACACAGTTCAAAATCCAATTTACAGTGATTTAATTACAGTACTTAAAAAATGTAAAATTGCCGCAGCAGGCCCTAATTACATGATTTTTATCTCAAAAAATGTACCAGAATTAGTTTATTTACAAGAAAATGGTTACAACTCAAATATTCAAAAATTTATTCAAGATTACTTTGAAGATTACAAACACTTATACTGCATTATGAGTGATGATTACAAAGAAACAGCAAAAGATTTTGCAGCTAAAATGAAAGATGCAAAATTTGACACAAGCTTTACAGTACCAGGACCAGTTGTTTTACAAAATGTAAAGAGTCCAACAGGCAAATTATTTGATAAATTCAAAAAATAATTTTTAGTTTATACTAAATTTTATTTATACACTTTTAAGGAGAAATTATGGATCAAGCAATGTTAAGAAAGATGCAAAAATTGCAACAAGAAGTTCAAGAAAAAACTGAACAATTCATGGAAGAAACATTTAAATTAGAAAAACATGGAATTGAAGTTACAGCTAAAGGAAGCAAAAAAATAGTTTCAGTAAAAATCAAAGATTCTATTCTTTTAGACCCAGATGATGCTGAAACTTTAGAAGACTTGTTATCACTTGTGATTAATGAGTTATTTGACACAATTGATGAAGAACAAGAAAAATTAATGCCAGCTATGCCTGGTTTAGGTGGACTTGGCTTCTAATAATGAAAATTGAAACAATTGAAAAACTTAATCAAGTTTTAACAACAATTCCAGGAATTAGTAAAAAGCAAGCAGACAAAATGTCAAACTTTTTCTTAAAACAAGATCAAGAATATATTGACAATTTAGTTAAGTCAATTAATGATCTTAAAACCAACATTTCATATTGTTCTATTTGTAACTTTTTAAAAGAAGGTGGCAAATGTTTAGTATGTGATGATAAGAGCAGATACAACTTTTTAATGATAGTTGAAAATGCTGCAACTTTAAGAAAAATTGATGACATGAATTTTTACAATGGCTACTACTATGTTTTGCCTTATTTGTTTAATTTAAAGAATAATAAGGAAATTAAAAACTATGATTACAAAGAATTAATAAACTATATTCAACATAAAAAATTTGATGAAGTAATCATAGTTTTGAGCCCTTCTTTAGAAGGCGAAATGACAACAGGTCATTTATTAAAATTGATTAATAGCCAATTTAAAATTAAAGTAACAAGAGCAGCTATTGGCATGCCTATGGGTTCAAATTTAGAATATCTTGATACTTTCACAATCAAACAATCTATTGAAAATAGAAACAAATAAAACTTAAGGGAGGTTAATTATGTTTATTACTTTTGAAGGACCAGATGGTAGTGGTAAAACTACAATAATTAACCGCCTTATTATTGAATTAATTGAAAAATATCCAAAGCTTGCATTAAATTATGTTTTGACTCGTGAACCGGGTGGCAAAGACATAATTGAAGCAGAAAAAATTAGATCATTAATTTTGGATAAAGATTCAAAGCTTTCTTCAGTTTCTGAAGCTCTTTTATATACAACTAGTCGTCGAATTCATCTTGAAAGAGTAGTATGACCCGCTCTAAAAGAAAACAGACTTGTTTTGTGCGATCGCTATGTCGACAGCTTTTTTGCATATCAAGGTTATGCTCGTAATTTAGGTATTACTTTTGTTAAAAAACTTACTAATTTAATAATTGATAAAACTATGCCTGATATTACAATCTTTTTTGAAATCAAACCTGAAGAAAGTAAAATCAGAAGAGAACAAATAAGAATTGTTAGTGACAGATTAGACAGTGAAACCGAATTATTCCACCAAAAAGTTTACAAAGGTTATCATGAGCTAATTAAAAAAGAAAAAGATAGATTCATTGTTGTTGATGCTACAAAAAGTATTGAAGAAGTTTTAAAAGAATCATTTGAAAAATTAGATAAACATCCAAGATTTAAAAAATGAATTAAGGAGTTTAAATAATGGTTTCAAAAAATGTCATAACTATTTTAGAAAACTCAATAAAAAATAACAAACTTAGTCACTGTTATTTACTAAAAGCTCCTTCAGGTTTAAACATCGATGATTCAATTTTATACATGATTAATAAACTTTGCAACTCACAATTGCAATCTTTAGATCTTGAAACAATGCTGCCTAATGTTCAAATTTTTGAAGATAATGGCACTGAAAATAATCTTAAAAAAGATAAAATTGTGGCCGGCTTTGAAGAATCAAGTTTATCTTCATTTGTTGAAGGGCAAAAGAAGATAACTGTATTTAAAAATGTTGAAAATGCTTCTAAAGCTGCTCTTAATTCATTACTTAAAATAATTGAAGAACCAAGTGATAATGTAGTCTTTATTTTAACAACTAATAACATAACAAAAGTTTTAGAAACCATCAAATCAAGATCAATAACAATTAACATTAATGCGCCTTCAAACGAAGAGTTAGCAGAAGAATTAAAAGGTGAAAATTTAAAAGAAGAAGCTATATGATTTTATAGCCATATTTTTGCTGATTTAACACAAGTAAAAAAATATACAAATAATGAATCATTTAAACTAATTAAAGACTTGCTTGAAGCTATAGAAAAAAGTATTAAAAATCCTCATTATTTATATATTTTCTTATCTAGATTTACAAAAAAAGATAGCAAAGATACCTTTGTATTTTTGGCTATGGCTTTGCGTTTTGTTTTCTCTTGAGTATGAACGGCTGATGCTTTTTGTAGAAAAAATTATGCTAAATTATTAGTTAAATTAAAAAACATAAAATTTGACTTATTCAACTGTTTTATAGTAATAGACGACTTTTTAAAGATTCAAAATACAAATGCAAATTTCTTCTTACAAGCTGAAAAAATGTTAATTAAATTAGTGGAGTCTTATGCCTAAAATATATATTGTTGGAACACCTATTGGAAACTTAAAAGATATAACTTTAAGAGCTCTTGAAACTCTTAAGGCTGTTGATGTTATTGCTTGCGAAGATACTCGTGTTAGTGCTAAATTATTGAACCATTATGACATTAAAAAACGTTTAATTTCATACAACAAAATCAATGAAAAAGCAAGTGCCCAAGGCCTTATTGATTTAATTGAAAAAGATGACTTAAAAATGGCTTTAATTAGTGATGCAGGTATGCCTTTAGTTAGTGATCCGGGTTTTGAATTAATTAAACAAGCAAGAGAAAAAAATATTGAATTAGAACTAATTCCAGGAGTCAATGCAGCTATTAGTGCTTTTGCTTTTAGTGGACTAAGTAATACCTTTATTTTTATGACTTTTCCTAAAGAAAAAAGTGGCCAAAGATTAGAACAAGTCAAAAGCTTCACAAGTGAACATGCATATGTTTTTTATGTAGCTCCGCATAAATTAGAAAGTTTCTTAAAAGACATTGACTCAGTGTGAGGCAATCAAGCTCAATTATTTATGGCTAGAGAGCTTACTAAAATACATGAAGAATTTATTTATGCAACCGCAACTGAACTATTAGCAAAAAACAAAACTAGTCCTTTTAAAGGTGAGATAACTTTAGTTATCAAACTAAAAGAAATTAAAAAAGAGAAAATAAATAAATATGCTCACTTAAAATAAAATTATTCTTATATAATTAAAGTCAACTTAATTGTTTATTATTTTTCACATTAAATGATTATTATAAAAGGAGATAATATGAATCATATTAATATTTTTGCTCTTGGTGGGCTTGATGAAAATGGTAAAAACTGTTATGTTTTCGAATTCAATGATGACATTTACATTATTAACTCAGGAACTAAAATTCCAATCAACTCAAATAATGGAGTTGATACTTTAATTCCTTCTTTTGAATACTTAGAAAAACACAAAGATAGAATTAAAGGTATCTTTATTTCTGATGTTAAAAATGAATCGTTTAGTGCATTGCCTTGATTATTAATGAAAATTCCTAACTTAACAATTTATACTTCAGCATTCAACAAAATCATGATCATGGATCGTTTATCAAAATATAAGATTCCAACAAAAAACTACAAAGTTATGGTTATTAATAAAGTTACACCATTTAGTGACAAACTTTTTGTTAAGCCAATCGATTTAGCTGGTTCAATGCCAGGACACATTGGTTTTGACTTCATCACACCAGATGGAGATATCTTATTTATGTTTAACTTTGTCGAAGGTGACTTAGGAATTTATGGTAAATTAAGCTTTAATGAACTTAAACAAAGATTTACAAAACGTAAAATTTTAGCTCTAGTTGTTGACTCAGGTAAAGCAAACTTTGGTGGTAAGGCTATTGAAAAAATTGGTTTACCAGAAGGTATTAGAGATGTTTTTTTAGAAACCAAAAAGAATGAAAGAATAATAGTTGGTGCTTATGACGAAGAAATGGTAGCTATCCACAAAATTTTAGAATTAGCTCAAGAAACAAGTCGTCCAGTTGTAACTTATGGTAAAACTTATGGCCAAATATTTTATTTAATTAAAAAAGCTCATCCTGAACTTAAGTTACCAGAATTAATTGATTATCGTAATGCTAACAAAGTTAAAAATGCTGTTATTTTAGTAACTGGTGCTACTGAAAGACTTTACTCAAGATTTATTAGAATAACTGACAATAATGATGTTTTTTTAAAACTTCAAAAAAGCGATACTGTTGTAATGATTGCTCCTCCTATTAATGGACTTGAAAGTCTTGAAGCTTTAACTTTAGATGAAATTGCAAGAATTACTCCAAAAATTAGTGATGTTAATGCTAATGAATTTTATAGATGTCGTCCTGCTAAACAAGACTTAATTGATTTAGTTAATGCTCTAAATCCTGAATATGTGATTCCTGTGCAAGGACTTTATCGTTACTTAAATGAAGCTACTATAGCTATAAGTGAAAATACACAAGTGAAACAAAATCATTGTTTAGTTATGCAAAATGGAAAAATTGTTCACTTTATTGATGGCAAAATGTCAACAACCAAGGGCAAAGTTAAAGAAATTGGCGACACTATTATTGATGGTTTTGGTGTTGGCGATATTAGTACTGAGGTTATTAGTGAGCGTGAAGCTTTAGGTCGTGAAGGTGTGATTTTAGTGTCTTCTCAATATAATCCAAAAACAAAATTATTAACTGGTAAATTACAAATAAATTTTGTTGGTGTCATAAACAAAGAGGAAAAAAATGAAGCTAGCGACATAATCAAAAGCACAATTGTAAAATTAATTGAAACAGAAAGTTTTAATGGAATAAAAGATTTCCAAAATGAAGCTCGTCATGCTATTAGAAAAAGAATTTACAAAACTTTTGAAAAAGAACCAATAGTAATTGTAACATTGGCTCAAATTTAAAATCATAAGTTATAAATTTAGAATTTAAGCAAAAAATTCAAAAAATAATTTACAGAATACTTTTACTAGCCTTATTTATAAGTATAAAAGTATTTTTTTTATATATAAAAAGTTAAACATAAACTTTGTTTTGCAAAAGCATTTTTAATAACATTTTTTAAAATATAAAAGCCTATTTAGCTTAAATATAAATATCTATGTTTTATGCGTTTATGATGTTCTATGACGCTTTTTTTAAATAACCAATAATTTATCTTTTTAGTTGTTAATTCAGTTAAAAATGTGACGCAATTTTCATTTTTAATATAAAAATTAGTAAAAAATTTTAAATTTTTTTAATCTCATATTATTAATAACAAATTTAATATTGCCACATTATAATTTTGCACATATAAACTAAAAAATGAAATCACAAAACAAATTGCAATTTTTTCTTATTTTTTGCAAAATTTGCCAATAAAAACCAATTTTTGAGATATTTAAGCAAAATATCTATATAATGTTCAATATACAATTAAAAATAAGGAGATTATATGAAAAAGTCAAAAAAAATTTTATTAGGATTGAGTCCTATTGCTGCTGTTCTTCCTGCAGTAGCTGTTTCTTGTGGAAACAACGATGAATCCAATATTTCATTCAAAGAGAAAGATATTAGTAAATATACCACAACAAATGCTAATGGAAAACAAGTTGTTAAAAACGCTGAATTGTTAAAATTGAAACCAGTTCTTATTACAGATGAAGGTAAAATTGATGATAAATCATTTAACCAATCAGCTTTTGAAGCTTTAAAAGCTATAAATAAACAAACTGGTATTGAAATTAACAGTGTTGAACCTAGCTCAAACTTTGAAAGTGCTTACAACAGTGCACTTTCAGCCGGACACAAAATTTGAGTACTTAATGGCTTCAAACACCAACAATCTATTAAACAATACATTGATGCTCACAGAGAAGAACTTGAAAGAAATCAAATCAAAATCATTGGTATCGACTTTGATATTGAAACAGAGTACAAGTGATTCTACTCATTACAATTCAATATTAAAGAATCTGCATTTACAACAGGCTATGCAATTGCAAGTTGATTAAGTGAACAAGATGAAAGTAAAAGAGTTGTTGCATCATTTGGTGTAGGTGCATTCCCAGGTGTTACAACATTTAACGAAGGTTTTGCAAAAGGCATTCTATACTACAACCAAAAACATAAATCAAGTAAAATTTACCACACATCACCTGTTAAATTAGACTCAGGTTTTACTGCTGGTGAAAAAATGAACACTGTTATTAATAATGTTTTATCTTCAACACCAGCTGATGTTAAATACAACCCACATGTTATCTTATCTGTTGCTGGACCTGCTACATTTGAAACTGTAAGATTAGCAAACAAAGGTCAATATGTAATTGGTGTTGACTCAGACCAAGGTATGATTCAAGACAAAGACAGAATTCTTACATCAGTTCTAAAACACATTAAACAAGCTGTTTATGAAACATTATTAGATCTTATTCTTGAAAAAGAAGAAGGATATAAACCATATGTAGTTAAAGACAAAAAAGCAGACAAAAAATGAAGCCACTTTGGAACTCAAAAAGAAAAATGAATCGGTGTCGCAGAAAACCACTTCTCAAATACAGAAGAACAAGCAAAAATTAATAACAAAATTAAAGAAGCAATTAAAATGTTTAAAGAATTACCAGAAGATTTCGTTAAATATATTAATAGTGACAAAGCTTTAAAAGATGGTAATAAAATTGACAATGTTAGTGAAAGATTAGAAGCAATTATTTCTGCTATTAACAAGGCAGCAAAATAATTAATCAAAAAAATGCTGGAAAATATCCAGCATTCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCAATAGGACAAATAAAAGTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTAAACCCCTTTGAAAAGAAGCATATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTCAAATTTCATGGATTTTTATTAGGAATTTTTACCAAAAACCCATATCTAGAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTGCCTTTACTCTTGCCTATAATAAGGTCTATTTCCCAATGGCCGAATTCTTTTCTTTCGTTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCGCCAACCAATCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGACTTTTCTTAATAAACCTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTTTTCAATTGTTAATTTGACAGTTCACAATTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATCTAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGCTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCTTTGGGTGACTTTTTTTATAAAAAAAACTTCACCCATTATAGATTCTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAATGCTGGAAAATATCCAGCATTTTTTATTTTAAATATGAAAAAAGTATATTTTTTTGTTAATTTTTGAAGAAATTAGATAAAACAGTTTTTCCGTTTTTGTCTTCAAATAAGATAAATAAGAGAAAAAAGGTTGTAAAACTGCCTAAGAAAAAAAGAGTTGGAAAACATAAAAGTTAAAGACCAAATTATTTAACTATTTATGACTTTGAGATGGATACGGTTCATGGGTGTAGAATGGATGAATATTTCATTTTGATTTTAATAAATAGATTGACTAGAGAAGTACACTTTAAAAAAAGATAAAAAAATTCTTTATCTACCAAAATAACTTTATAGGAAATCATATAAGAAAATAGCCTTAAAATCAATACATTAACTATTGATAATGGACCTGAAAATTATAGGCTAGATGAAATCGATGCCGTGGGCCAAATATATCATTGCGATGCTTTTTGTTCTTCATAAAAAGGTCGTGTTGAAAACTACAATAAATTTATTAGAAAATATATTCCAAAAGGAAAAACATTAGACAATTTATCAAATAAATATATTGCGTGAATTCAAAATGAAACTAACTCATTCATAATAGATAAAATTAAAGAATATCCATATGCAATGAGTGCAAATGATTTTGCAATTTAAGTTATGATGAACAAACAATATAACCAACAATGATTTATCAATTTATATCCAAATATTTAAAAATAAAAAATAAATATAAAATTACTAGCTTTCGCTGAATTTTGCTAGCTTGCATTAAAATATTTTCTTTTGCGTTTTTTTCGCTTTCAGTCAAAAAATGACTTTTTAATTTAAAATTTAGCAATCGATTTAGAAAAATGTTTTTCTTAAAACTTACCACACTCACTTTTGGAAAAAATAATAAACTATATTTTTAACATATTATTTCTTATTTATCTATTAATTGATATACTTAAATAGTTAATTTTATTATTTATATTAATATATAAAAATATATATAAATATGAGTCTGAAACAAAATTAACTAAAAATTTTATTTAAGAAAAGGACAAAGATGAACGCAATTGAATTTATTAATATTTCGAAAAGTTTCGGTGTAATCAAAGCTAACCAAGATATTAATATTCAAATTAAAAAAGGTACAATTCATGCACTTATTGGTGAAAATGGTGCAGGTAAAAGTACCTTGCTATCAATTCTTTTTGGACTATATGAACCCGACCATGGAATTATCAAGGTTAATGGTAAAGAGGTTTTAATAAATAATCCAAATGATGCTAATAATTTGGGTATTGGTATGGTTCACCAACATTTTAAATTGGTTGGTGCATACACAAACTTACAAAATATAATTCTTGGTGCTGAAAGTGAATCAAAAACTTTTAAAACTTTGGATTATAAACCATCAATTAAAAAAATTAAAACTATTCAAAGATTATTTAATTTAAATTTTGATTTGAATAAAGACACAGGCACTGAATCAGTTTCAACACAGCAAAAAGTTGAAATCATGAAGATGTTATATAGTGATTCAGAAATTCTAATCTTTGATGAACCAACTGCTGTTTTAACAGATGAAGAAATTCAAGGTCTTCTTGAAACATTCAAATTGTTTAAATCGCAAGGTAAAACAATCATTTTTATCTCTCACAAACTAAAAGAAGTTAAAGCTGTAGCTGATTATGCAACAGTTATACGTCACGGTAAAGTTACTGGTAACTTCGAAGTTGCTAATACTTCTATTGAAGAAATGGCTAACAGAATGGTTGGTGACAATATTGTTATGGCACAAAACCAATCACTTGACGTTTTTGAAGATAAAGACACAGTTTTAGAACTTGAAAATGTATCAACAAAAGGTACAAAATCACTTAAAAACTTAAATCTTAAGGTTCATGCTGGAGAAATTCTAGCTATTGCTGGTGTTGAAGGCAATGGACAACAAGATTTAGAATATGTTGTTAGTGGAATTGTTAAACCTACAAAAGGTAATGTCTACTTAAAGAAAACACCTTTAGTTGAGGAAAGAGTTAAATTCATTCATAAAAATAAAATCAAAAATTTCATTATTTTAAGTTCATTGCTTGCATTATTTATAGCTTTTGGATCAATTTTTTTTGGTGTTAGTCCATTTCAGCAAAATTATTCAAACATTTGATTAATGTGAGCTATAGGCATTAGTTTCTTTGCTCTATTTGCTGTTGGTTTAAGTATCTTGATAGTAAAAATTATTAAATTCTTTAAAACCAAAAAAGATGAAGAGAAACTTAAAGACAGGATTAATTTAAACAATTATTCAGTCTTAGAAAAATCAATGCTTGCTTCAAGTTTTATTCCAAGTGATAGACATAAACATGGTCTAGCATTAGATTACAATATTAAAAATAATACAATATTAAGACGTTTATGAGATCCAAAATATCAAACTTTTGGTTTCATAAAACCTAAAGCTGTTAAAAAAGAAACAGCTGAAGTTATTGACAAATATGACGTTCGTGGTGCTAGAGAAGGTTATTCAACTTCTAGATCACTTTCTGGCGGTAACCAACAAAAATTCATTGTTGGTCGTGAAATGAACTCACCACACGACTTTATATTAATTCTTCAACCAACACGTGGACTTGACGTTGGGGCTATTAAAAATATCCATGAAAGAATTCTTAAAGAAAAAGCAGAAGGAAAAGCAATTCTTTTAATTTCTTATGAATTAGACGAGGTTCTTTCACTAGCTGATACTATTGCTGTTATTAACGAAGGCGAAATTAGACTTTCAAAACCCGCAAGAGAATTAACTAGAAAAGAAATCGGTCTTTACATGGCTCAAAAAGAACATCAAACGGAAGGAGGCACACAATAATGAAAGAAACAAAAGAAAAATTATCATTTTCTTCTAAAGTAAGAACAGGCTGAAATAAATTTAGATCATTTATTACACTTGAAGATCAACAAAAAGGAAGAAGAAAATTATACTCATCATTATGAGCTGTTGTTTTCGGATTAGTTGTTGCTAGCATCTTCTTCATGATTCATCAAAGCATGGAAGGACGTAGTGCAGGACCATTCGACTTTGTTATAAAATTATGAAATTATAGCTTTATTGACAAAAACATTAAATTTGACTTTGCAACATTTTTTGTAATATTTGGTTTTACTGGTTTAGCTGTTTCAATCGGTTTTAAAGTTGGATTATTTAACATTGGTATTTCTGGTCAAATGCTTTTACCAGCAATCTTAGTATTCAGTTTTATAGCTAAAGCTAGAAACTTTAATGTTGACGCAGGATTCTTATGATTAGCAATTATTTTGTTCATAATCGTAGGATTTGCAACAGGTGCAATCGCTGGATTATTAAAAGCATATTGCAAAATTCACGAAGTTATTTCTACTATTTTCATTAACTGAATTGTTGCCTTTATATCAGGATGATTATTTAGTTTAAATGGGAAAATGTTTAATCCAGAATCATATTCACCTACAACTATAGGTAGTGCTAGAATTGCAATTGAAAATGCAGTTAAATATCAATTTGTTTACTTTGGTATTGGAATGTTTGTTTTAACAGCTATTGTTTTATTTGTGATCTACAACTTCACAACATTAGGTTACAAAATAAACATGGTTGGTAAAAATCCTTCAAACGCTAAATATATTGGTCTTAACGAAAAAATGTTAACCATTATAGTTATGGGATTTTCAGGAGCTTTAGCTGGTTTCGCTGGATTCTACTACATTATTATCAAAGAAGGCTGAATTGTCGATTCATTTGGTAGCAACCCTATTGCAATTGGTTTTGAATCAATTGCTGTTTCACTTATTGCATTAAACCACCCTATTGGTATAACATTATCCGGATTTTTCTATTCAATGATTTATACAACAAGAGATTTATTTGCTATAAAAGATAAATTAACAAAAGACTTTTTCCCTATTATTACTGGTATAATTGTCTTTATGGCTGCTTTAGCTATTATGCTTTATAAATTCAAACCACTTGGTTTCATGTGAAAATACGGAACGCTTTGAATCAAAAAAGAATACTGAAAAGAATATGGTTCATACATGAAAACCAAAAATGCTAAATATAGAGAATATGTTAAAGCTCTTAATGAAGAAAAAAGAAGGAATAGAAAACTCAAAAAAGAATTTGCTTCAATTAAAGAAGAATATCAAAAATGAGTTGATTGTAAAATAACTTCACTTAAAAGCGCAAGTGATGATACTATTATGTCTATCTATGACGAAATGTCTAAGAAAAAATTCGAATATCTCAAAAAATACAGTGATTTTGGCTTAAACAATCTTAAAACATTAAAAAATCAATATAAAGCTGAAAAACACGAAAGAAAAATTCAATATATTACTAAAAGAGATTTAATTTATACATCTCATTGAGACAAAGTAAAAGAAAATTGAGCTAAAAAAAGAGATATGAAAAAAAGCAATCAAGCAAGTAAAGGAGGTGAATAATGTATAGTTGAATATTAATTTTAACTACAACCATTTTTATGTTTTCAGCTCTAACACTTGCAACCCTTTCAGGTATTTTCTCAGAAAGAGTTGGTATTACAAACATTGCTGTTAACGGGTTTATGATTTTTGGAGCTGCTATGTATGCTCTAATAAGCTTCCTAATGACTGATGTTGTTTTCAAAGGTAAAACATTAAGTCCATGATTCCAATTATTGTACTTTGCTTTTGCAGGTATCTTATCCTCGGGATTTGCACTGTTGTTTGGTTTCGCAACGATTAAACTTAAATCAGATCAATCTGTTTCAGGTTTCGCTATGAACATTCTTGCAGTTGGTGTTGCTTCTATCCTTTTATTATTTGTTAAAAAAGCACAACAAGGTGGAGACTACTTCCAAATGCGTGGCCAAACAGAACTTGCTTTAAGTCCTAATGTTGACAACTGAAAAAACATTATTTCACTTAAACTATTCTTAGCTTTAATTATTGCTGGTGGTTCTTGATTCTTCCTTAACAAAACTAAATGGGGTTTAAGATATAAAGCTATAGGTGAAAATCCTCAAGCAGCAGACGTTGCTGGTATTAACGTTTTTAAATACAAATGATTAGGTATTGCATATTCAGGTTTTATTGCTGGTTTAGCAGGCGCTATATTTATTCAATGTAAAACAAACCAATTCTCACTATACATCGAAGTTGGAGGTTTAGGTTTCTTAGCTCTAGCTATTATGATTACTAGTGGTTGAAAAATTAGTTGAACATTATTGATATGTATCATTTTCTCATTTATCTATGGATTCTCATACTTCGGAATTTCATGACTTGAAAGATTCGAATCTATGAAAGGTGTTTCAAGATTCGCTGAACTACTCTACACATTGCCATTTATTATCACACTTGGAATTATGATTGCTAAAATGAAAAGCAAGCAAATGGGGCCAGCTGCTGCAGGTCGACCTTATGACAAATCAGAGCGTTAGCTATATAAGGAGATTATTATGAAAAAAAGAAAATTTATATTTGCTTTATCATCATTAACAACAGCTTTTTTAGCTACACCTCTTATTTCTTCATCATGTACTAATGCTGATAAATTGTCTAATCTATTAGATGATTTAGACATTACTATTCCAGATTCAGAATCTAAAAAACCAAATGAAATCAAAGAATCGGATTTAAAATTTTACTCAAAAAATATGCCAAGTTTTAATAGTGAATATACTGTTGAAATACAAAAAATTCAAAAATATTCTTCAGAACTTGTAGTAACTTTTAGACTACGCGAAAAAAACTCAAACATTGTTTCAAAATACAAAACAATTACATACAAAAATTTCTTAAACTTTGATCATATTAATGAAGCTTTAACAGTACAATATTCTAATTCAAAAAATACTTATGTAAAAGATGTTGCTCAAACAAGTGACATTATCTTTAGAGATATTAAAACAGGATACAAAATTATCGATGTTAAAATTGATAACTCAAGAATTGAAGAATATCAAAAAAATTTATCTGACAAACTAAAAGATATAAATGAATTCTTAACAAAAAATAAATCTGAATATGATGCTGAAAAAGCTAAAGCAAATCCTAATAAGGAAATTATTGAAAAATATGAACGCGCTTTAATTAGAAAAGCTAAATATGAAGATACTGCGGATAAAGATTCATATATCAAATCATTCGAGAATTTAAATATAAAATACAAAATTCAATCAATAAATGATCCTAAAATTGTATCTGATGAATTCAGTCGTTCAATTCCAAACTTCAAAAATGATTCATACATTGATTACTATGTTTTTGATAAAAGTGACACATACTCAACAGTTTCGCATTCATTTCACTCTGTTTCAGATTTAACTGAAGAAATAAAGAAATACATTAAAAAAACTGCTTCACTAGAATATACAGGAAATACAAATGTTTCTGTATCTGAAGTTAAGGCTGATAAATTTAAATTTAATTTTGACACACCAATTGATTTTTCAATAGAAAACATCAAAGTTGAACCTGCTGATTCAGAAGGCAAAGTAAATCTTAAATTTAATATTAAAGCACCTATTAACAAACAAGCAACAACTTTATTAGTTGATGAAAACAAAAAAACAATTGAATTTGATTATGCATGTGAAATAGAATTTAACAAAAAAGATAGTAAAGAAAAATTAAGTGAAATTGAAAAACAAATTAGCTTTACATATGAAAATGCTGCAACAACAGAAGTTGATAAGGCAGAAGTTTCTAAAGTTAAATACACTTTATCTAACCCAAATTATGAAATTACTGATTTAAAAATTATTGGTTATGAATTCAACAATTCAACTATTGTTATTGAATACAAAGCAAAAAGTAAAAGCAGTTCAATTGAACAAGTTTATTCAAGAACAATTAGTGGCTTTTCTAAACTTTCACAATCTAAAATTGATGCAATTTTAGATTCAATTAAATTGAATTATAAACGTAAAAACTTAGTAGCTGCAAATGAAGCGAATAAAGATCATGTATTTATTGAATCAATGGATTCAAACTTCATGATTGAAGTTACAAAAATAGATTCATTTGATCTTAAAGATAAATCAATATTAGTTGAATTTAAATTATATGAAGTTGATGCACTTTCTAAATTAAAACCAGACAACAAAAAACGCGGTTTCAAATTTGTTAAATCATTCACAAAGAAAATTACAGGCTTCTCAGAAAAATCATTGACACAACATATCAATGAAGAAATTCAAAAGGTTACTTGTACTTGTCCAACAGCTTCAAGCAAATTGCCAAGTCAATTAAACATTAATGATATTAAATTTACTAACTTTGATAACAATATTTTTGAAATTGAAAAACCATCAATAATTGTTGCTAATGATCAAAAAGGCGAAGTTGTTATTACATTTAAATTACGTTACAAAATTGGTTATGTACAAAAAGTTTATGACCAACAAAAGAATGAATATGTTGAAAAAGAAATACCTTTCAAAACTGTTTCAGACTCAAAAACAGTTATATTAAATGGTTTTAAAAAAAATAAATTATAAGGCTCATAAAGCCTTATTTTTTATGCTTTAGGTTTAAAATAAATTTAAAAAAACTCAAATATTAAATTTAAAACTTATTTGAAGATTAATATTTGACTTCGAAAAATCAAAAAACAAAAACGAATATTTTCTTTTCAAAACTAATTTTTGCAAAATTTTTAACTAATTTTTAAAATGTGGTAAAAATTACAAAAAGTGCATTTTTGATTAAAAAATAGGTTAAAAAATAATTTTTTATTTTTTAACAAAAGATTAAAAATATGTTAAGATTAAGTTACTTAAAAAAATGAATAAGGAGATTTTATGTTAAAAGAAATTAATCAAGATGAATACAAGAACAATGTTATTGGAAAAGAAAAAGGCATTTATGTTTTGGTTTTTCACGCATTATGATGTCCTCCATGTCGAATGTTTAAATCTTCTTTAGAACAATTAGACAAATTAGACAACATTCCAGTTTATCGTGTAGATGTTGATGAAAATACAAAGCTAACTCAAGAAATGGGTGTTAATTCAATGCCAACATGATTCATTTTTAAAAATGGAAAAATGATGGAAAAAGTTATGGGCTATGTACCTTATGATCACCTTAAAGCAAAAGTTATGGAATACAAATAAAATAATTAAATAGGCTAGTCCTATTTTTTATTTATAATATTTAAACTATGAAAATAATAAGCGACAACAAACGCGGAATGCACAACTATAAAGTAATTGACAAATATGAAGCCGGCATATCTTTAATGGGTTGAGAAGTTAAGAGCGCTCGAGCTAATACTGTTAGTTTATTGAATTCATATTGCTTTTTTAGAAAAGGCGAAATTTTCTTGTGCAATGCACAGTTCAAACAATACATGTTAGTTAAGTGTGATGAAACTAGAGATCGCAAATTATTGATGCATAAAAATGAAATAGTTAGATTGCAGTCTAAACTTCATAAGCTAGGTCATGCAACTATTATTCCAAGCAAAATTTATTTTGATAATCGCTCACGTATTAAGATTGAAATAGCTTTAGTTCAAGGAATGAAAAAAACAGATAAACGTGAAGAAATCAAAAAAAGAGATAATGAAAGATACATCAAAAAAGTCTTGAAAAATGTATATTAAAATGACGCTAAAGTGCGTCATTAATTTTTTAATTATCGAATAAAATTTTTGGTTTATAAATTTTGTTTTCAATTGTGTTATCAACGTCACAACTATAAAGCATTTTTTCAATTTCTAATCCAACTGCAGGATAATCAATAAAGATTTTAGCTTTATATTTGTGTTGATAGTCATAAATTGAAACTCAACCAATATCAGTTAATCTTAAATTACGATCGCCTGATGAAATTAAGTTAATAAATACTTCATGAGTTGAGCAAACAACATTAACCAAATTGTTATTTCTTAAGTAATTTAAGAAAAATTGTACTTCTTTATTATTTTTGTTTTTAAGTGAATAGATTTCATATTCTACATTAAGCTTCTTGCAAGCATTTTCAAAACCTATACAACGAAGTCTTTTTTGTTCTTCAGTTAACTTAACATCTTCAACAAAAACAACCTTTTCGCCTTTATCTATATAGTTTATGAATCTTGAAGTTAAATGATGAAATGAGTTCTCTTCATCAATTTTTATTCAATTTAATGTTTCTACTTCTTTTCCATAAACTAAAATATTTGATTCAAAATTTGTGTTCTTAATATAGTTTGTAATTGTGTTTTGAGGATCTTCAGGAAGAAAGAAAACTATTGCCATAGGTTTTCAGCTTGAAACATATTTGATAGTTTCAATATATTCTTCAGGATTAGGTGAAGAGTGAGTTATAACAACTTTTCTATTTCTTGTCTTTGCTCCTTGTTCAATTCCATTCATAATTTGAGTATATGAGTTTTCATATCACTCAGGCACAATAATAAAGATAGAAGTGTCACGGCCACGAATTGTTCTGGCTCCATGATTTGGATAATAATCGTTTTCTTTTATGATATTAGCAATAACTTTTTTAGTTTCCTTGCTAACATAACCACCACGATAGTAACGGCTTACAGTACTAATAGAGACACCTGCTTCTTTAGCAATATTTTTATATGAGAAATTTTTCATATATTAATTATATAGAAAAAATTAAAATGTGAAAATTTCCACAAAAAAATTTATATTTTTTTTAAATAGTGTATATGAATAAGCAAAAAACGATCTTTAGACCAATAAAAAAAGCCTGACTTAGTCAAACAATTTGAAATAAATAAATTTTTGGTATAATTGTAGCGTCAGTAGAACAATAACCTTGTAACCTGGTCAGGTCAGAGATGAAGCAGCCATATCGAGTAGTGTTGTGTCTATTGACTTTTTTTATTTTTCTAAAGCTACTAATATTTCATATTCTTTAATTAGCTTTTGATAGATCTTTTCTTCTTCATTTTTCAAATGCCTAAATTGTTAATAAATATTTCACTTGGTTTATGTCTTTTCATACTTCAATTTTAAATAAAGTTGACTCAAAATAAAAAATGTTTTGATTTTTAAAAAATGTGTTTATAATATTAAAAGCAAAAACGCGAATGTAATTTAATGGTAAAATGCCAGCTTGCCATGCTGATAATGGGGGTTCGATTCCCCTCGTTCGCTCCATTTATTCTTTATGAATGCCACACCTGTCTTCGACAGGTTTTTTATTTAAAAATCTGTGCAAAAACTAAATCGTTATTTTTTGATTTGACCTTTAACATTATATTTTTTAAATTAAAATTTATATATATTCTTATGTTAAATTAAAAGTTAATAAGTTTATTTTTTAAAATATTTTTTTAATATATTTTAAAAGATAATAAGTATTAATTCTAAAAGTGAGACTTCTAGGTTTTAGAGATCTCACTTTTAGAATTAATAAAACAATGAGAAAATTTATCAAAAATTTTTTGTATATGTAATTATGTTAAATAAAAATACCACAAATGTGGTACTTAAAATAATTATTAAATGTCAATATTCTTTACATATTTAGCGTTTTGTTCAATGAATTCACGACGAGGCAACACTTCATCTCCCATTAATGTTGTAAATGCTCAGTCAGCTCTTGCTGCATCTTCAATTTGAACTTGAAGCATTTTTCTATTAACTGGATCCATAGTTGTTTCTCAAAGTTGTTCAGGGTCCATTTCACCAAGTCCTTTGTAACGTTGAACTGTTACTTTTTGATTTTGATTTAAGCCTTGCATAATTTCTTCTTTTTGAGCATCGTTGTAAGCATATCCAATAAATTTGTTTTGAGCTATTTTATATAAAGGAGGTTGAGCAATATAAATAAATCCGTATTCAATTAAAGGTCTAAAGTAACGGTAGAAGAACGTCAATAACAATGTTCTAATGTGAGCACCATCGACATCGGCATCGGTCATGATGATAATTTTGTGATATCTTAATTTATTAATATTAAATTCTTCACCGACACCTGTTCCAAGTGCTGTAATTAATGAAAGAATTTCAGCATTTGCAAACACTTTTTTAGGTTGATTCTTTTCAGCATTAATAACTTTACCACGAAGAGGCAAGATAGCTTGAGTCATTCTGTTTCTTCCCATTTTAGCTGAACCACCAGCTGAGTTACCTTCGACAATGTAAAGCTCACTAATTTCAGCATTTTTTGAAGAACAGTCAGCTAATTTTCCTGGAAGTGAACCTGTATCAAAAACAGTTTTTCTTCTAGCTGCATCTCTAGCTGCTAAACCAGCAAGACGAGATTTTTTAGCAAAAATTGTTTTTTGAACAATAGTTTTTGCTTCAGCAGGATTTTCATTTAAGAAACGTTCAAATGAATCTGAAAACACTTTGTTAACAGCCATTCTGGCATCTTTGTTTCCTAATTTTCCTTTTGTTTGGCCTTCGAAAATAGGGTTTGTATGTTTAACTGAGATAATAGCAACAATACCTTCTCTAACGTCTTCACGAGTAACTTTGTCTTCTTCAGTTTTGATTAATCCACATTCAGTTCCATAATTATTTACTAAACGAACTAAAGCATCATAAAAACCGCTTTCGTGTGTTCCACCTTCAACTGTAATAATGTTGTTAGCATAAGAAACTACATTTCCTGTAATTCTTTCATTATATTGAACAGCTACTTCAACCTGAACTGCTGGAGAATCTTGGAATGAGTATTCACCTTCAGCATAAATAACATCGGGATGAATTAATTTTTGACCTGTATTTAATTCTTTAACATAATCAACAATGCCACCTTCAAAGTGATATTCTTCTCTTGATTTGTCTCTTTCATCATTAACAATAATTCTTAGGCCTTTGTTAAGATATGCAATTTGTTTAGCATGATCAACAATAACACCTTTATCTCATTCGTTTTTGTCCATAATGCTAAAGTCTGGATGGAATTTAATTCTTGTTCCTGTATCTTGAGGATCTACTTCGTTTAATATTGTAAGATGTTGAAGAATTTTTCCACCATTGTTAAAATGAGCATAGTGTAATTTACCATCACGTTTAACTCAAACTTCAAAGTCATCGCTAAGTCCATTAACTACTGAAGCACCAACCCCGTGAAGTCCACCAGAAACTTTATATGAGTCAGAGTCAAACTTACCGCCAGCATGTAAAACCGTTAAAACTGTTTCAACTGTTGAAAGACCAGTTTTAGGGTGAACTGCTGTAGGAATACCACGACCATTATCGATAACATCAATGTAACCATCCTTTGTAATCGTGATTTCTACTTTGTTTGCAAAACCTGCCATAGCCTCATCAACAGAGTTATCAACTATTTCTCAAATAAGGTGGTGAAGACCTCTAATTCCTGTTGTACCGATGTACATACCAGGTCTAACTCTAACGGCTTCTAATCCTTCTAAAACTTTAATATTCGATGCATCATATTCTTTAGACATATTGCCTCCAATTCTTTATATTTTTTAATATAAAGATTATACCTTAAAAAGGTGAAAATTTTATGGAATTTTTCATAAATTTTTTTTAATTTTATTTTTATAAATTTCAATAAATAAAATTTTTTATTTATTTAATTAAAAGACTATTCTATAGGCTTTTTCGTAAAAAATGACTATTTTAATAAAATTAAAAATATTTAAAAATAACTAAAAAATTGCACTTACAATTATTACAATTAATTAAGGTTTATAAATTTAATAAAAAATAATGTAAAATACTATAAGTGCTTTTGCGCGTTTTTTTAAAATTTTTGAATAATTAAAAGAAAGGAGTTTATGCCTAATAAATCACAAAATGATGAAAAATTAAATCAATCATCAGTCGAAGAACATAATGTAAATGAAGAACATACACAAAAACAAAAAGACAAAGAAGTGATGGACGAGGTTAATGCATTCGAAAGAGACGTTTTACTTAATAATGGTAGAGTCGAAGACAAGCATGTTGAAGAACATCATGAAATGAATTCAATTCATTTAGATGACGTTGTTAACAAAAAAAATACTCAATACAAATACACAAAAATGTCGAATTTTGTGTTGAAATTATCTCGTTTTTATGCTCCTATGCCTCTTTGAAAACTAGGTCTAATCACAGCTGGTTTTGCAATTGTTTTTGGTATTGTCGGAGTTTTCTTTGTTAAAAACCCAGGAATTTATAACTTTGGTTTAGCAGCTTTTGGACAAGCAATTTCAAGATTGACTAACGTTCTTTTAAGATCAAACCCAAACATTAATGACACTATTTATAACGTTATTGACCACGCCTTGTTCTGAATTCTTTATCTTGTTTTGAGTATTCCAATTTTTATATTTGGATGAAAAAAAGTCGGTAAAGTTTTTACTATATTAACACTAGAATTTCTAGTTGTAAGTAGTTTAGTTTCATTTGGTTTAGGTCAAATACCAGGGGCTAACTCAGTTTATATTATTGGTAACTTTTCACACAGTGATATACCAGAAGAATTAAGAAAAGCAGTAGCAGACAAATATTGACAAGGAAAAGAACAATTATGATCACTAGTTCCTCTTCAATGAAATGATGGAGGTAACATAATTGCTCAAATAATTTTTTCTGTTGCTTATGGTTGAATGCTTGCTTTCTTCTTTGCAATTATTGCAATAATAGGAGGTAGTGCAGGAGTTACTGGAATTATTGGCGAATATATGGCTGTCGTTAAACAAAAAAACTTTGGAACAATTAATGGTTATATCAACTTAGTAATTATTATTGTTTCAGTTGTACTTGGTACATACTTACCAGGAAGTTTATTATTAAATGACATTAATGGTTTTAAAGCAGATTCAGTGACTTGAGAAAATACAACAGTAGAAAAAGTTGAAGAAATTCTTAAATCTCTAAAAACGTTACCTTGAAAACCATCGCTTTACTTCTCGCCTAACTTTATATCAACATTTATTTGTAACATTATTTTTGTTATGTACCTTAACAAATTATTCCCTCGTTACAAAATTGTTCAAGTTAAAATTTACTCGCCTCACATGAGTGCTATTAGAGAAGCAATTATTAGTGATAATAAAACTGTTAATAGCTTTACTATCACTAAAGGCGTTGGAGGTTACTCAGGAAATAACACCAAAGTTCTTTCATCAATTACTCTTTACAATCAAGTGCCACGTCTTATTAAGAAAGTTCGGGCCGTTGATCAAAACTCGCTGATTACTATTAGTAATGTTGCTAGTGTTGATGGAAAAATATATCTTCCTGAAAACAAATTCTAAAATTACAGCTGCGGCTGTTTTTTTATTTCAAAAATAAAAGTAATATTTAATATAATAATAAATACTATGAAATTAAACTATATTGAATACAATGGCGACAAAGTTCCTTTATATATAGAAGATAACAAAAAAAATACAACTTTTTTGTTTCTTCATGGACTTAATTCAAACTGAAAATACATGAGACCATTATTAGATTACACACATAATTACAATGTTGTGGCTATTAATTTTCCAGGAAGTAAATTTTTCAAAAATGTAGAACCAGAAGAAATTAAACTTGAGTGATGAATTGAAGTAGCTAAAGAGGTTTTAAATAGAATTAAAACTAAAAAAGTGGTCATAGTAGCTCACTCAATGGCAGGTGGCGTGGCTACTGTTTTAGCTAAGGATCCTAAAGTTGTTAGATTAATTATGATGTCAACAATTAATCCTTATATGAAAGAATCTACTTCTTATTCAGTGTTACAATCTGTTATTAAACCTCAAAATAAATGAAGCTCATTTATTGGCAAAGTTATTTCTAAAATAGCTTCTAAGTTTAAAAAAACTGAAAAATTAATTGAAAGTTTTACTCGACAAGGAGCTTGATTTAACTTACTTGAAAAATATGTTTTGAATCCTGAATATATGGAACAATTAGATTTACAATACAAAGCAAATTGTCACAAAATGCTCTTTTTAGTAGGTGAAAAGGACAAAATTATTGGTACTAAATATTTTGAAGATTATGCAATTAGTTTAGAAGTTCCAATTTTAATTATGGGTACGGGACACTCGCCAATTAAAGATGAACCAAAAAGAATTGCAAGATTTTTTAATGCATTGATTGAAAGCAAAAAAAGACACTTTTGACAAAAATTTATGAATATTAAAATGAATCAATTCTTAGAATTTGATGAAGAAAAAACAAAAGAAGAAGATATTCTTCTTGAACTAGAACAAAACGTTGATTAGGAGATTATTATGGAATACAATCAATTTGAAATGGAACTTAGAAGTGCTTTAACAACAGGCAATGGTACAAAACTTTTATACAAAATAATTGATGCACCTTATAGATTTTTTTCACCTTTGCAACCTTTTAATTACAAAGTTAAATTAGAACAAGCATTCTTAAGAACACAAGAAAACACATATGTAAAATTTATTAAATATTGTGCTGAATATTTAATTACTCAATATGGTTTTAAATTAGAAAAAAGTTCTTTTAAATATACTTTACCACTTAAAGAGGGAGAAGAATTCCCAGAAGAAGTAAAAGTCAATGCAGCTATGATTTTTAGACAAAAAGAAAATGGCGGAGATGAAGAAACAGCTGATATTTGTGTAATTTACTTGAAAAAAAGAGATTACTATGGAGCTAAAGAAGTTACTAAACTTCATGACAATATCATTAAGCAATTAGAAGTATTAAGAATAATTTATCCTAATAATAAAATCAAAGGTATCTTATGATTTTTAGATAATGACTATCACAAAAATGTTAACTTTTTTAATGAATGCTATGATAAAGAACATTCATTTGAAACACCTGGTTTTAGAGCTTATTATGGTGCACAATTTTTCAAAATTTTTGATAAAGAAGAAGACTGATTATTAGTTGAAAATCATATTAAAAAATTTAAAAATAGTAACTATGATTACTTCTTAAAAATGCCAGATTTAGATCAAGATGAAGATGTTTTAAATGCTATGATTAATTTAAATGAAACATCTTGACAAAAATTAATTTCACCTGATGAAGTTTATGCAAATATTAGACAATATATTTTCAATGAAAGAAATGAAAATTCTAACTTATTTAAAGCTCTAAAACTTAGAGATATTAAACGAACTTCAGTAGATGATGATGAAGAAAAAAGACGCATTTTAGAATTGGAAAATAAAGCAAATCAAAATGAATAGATTTTTCTAATCAATCTGTTTTATAGGTAAATTAAACAAAAAGCCAGACACTTCAAATGCCTGGTTTTTCTTATTATTATTTTGTAATTTTTGTTATTCCGTCCATATAAGGAACTAATTTCTTAGGTATAGTAATTGAACCATCTTTGTTTTGATAATTTTCGAGAATTGCTGCAATTAATCTATCAATTGCAAGACCTGAACCATTCATGGTATGAGCATAAACATTTTCACCATTTTCATTCTTATATCTAATCATTGCACGACGTGCTTGGAAATCTCCCATATATGAAACTGATGATATTTCACGATACTTAATTTCTGAAGGCAATCAAACTTCTAAGTCAATAGTTTTTCTACTTGAGAATCCTAAGTCACCTGTACATAATAATAATTCTCTATAAGGTATTTCTAATTCTTCTAAAACCGATTTAGCTTGTTCAAGCATTTGATTAAATTCAGCTTCTCAATCTTTTTCACTAACAATTTTTACTAATTCAACTTTTTTGAATTGGTGGTTTCTAATAATACCTCTTGTATCTTTTCCACTACTTCCTGCTTCTGATCTAAAACATTCAGTAAATGCTGTCATTCTAATAGGTTTTGATAAATCTATTATTTCATTATTATAGTAGTTTGTCAAAGGGACTTCAGCTGTTGGAATTAAATAACTTTGAGCATCTTCTAAAGCAAAAAGATCTTCTTTAAATTTAGGCAATTGACCTGTACCAAAAAGAATTTCAGGTTTAACTATTACAGGTACCGAATATTCTTCATAACCATTTTTGGTATGTAAATCTAACATAAAATTCATAATAGCTCTAGCTAATCTAGCACCTAAACCTTTGTAAATTACAAATCTTGAACCTGACATTTTTACTGCTCTTTCAAGATCGATAATGTCTAAGTCTTTAGCTATAACATAGTGAGGTTGAACGCCTGAAACTAATCCACGTCCTAATTTATCATGATTTTTTATAACTTTATTTTGGTTTTCGTCTTTACCAAGTGGCACATCTTCCATTGAAATATTTGGTATTCTGATTAAAATATCATTAACTTTCTTTTCAATATTTTTAGCAGCTTCTTCAAGTGCTACTTGTTCTTTTTTAATATTTGCTACTTCTTTTTGTAATTTAGCTAATTTAGTTTTATTGTTTTTGTTAGCTGCAAATTCTTTACTAAATTTTGTTAATTCAGCTTTCTTGCTTTGAGCTTCAAACATTAGCTTTCCACGCTTATTTGCTTCTTTAATAAGCTCATTAAATTGCTTTAGATCGAAATTTCTATTTTTTAAAGCTTCTTTAACTTTCTTTTCATTTTCTAAAACGTATTTTAAATTTAGCATTTTGCCTTTCCGCCTATTTTATAGGCTTATAAATCAAAAAATAACCTTAAAAATTCTTCCTTAATTTCATAAAATTATCTTACTTAAATAATACAACAATATTAAATTTTTAATACTTTTTTACAAAAATGATTTAAGACAATAATTTCTTTTGCATTTTTAATAATTAATATATAATTAAGGCACATTTTATTGAGATTTAAGTATTAATATTAATATTTGATAAAATGCATGCATAAAAAAATAATTTAAGGAGGAAATATGAGTTTAAGAACATATCAACCAAACAAGACCCAACACATCAAGACTCATGGTTTTAGAGCAAGAATGGCAACAACAAATGGTAGAAAAGTTTTAGCTGCTAGAAGAGCTAAAGGTAGAGCAAGACTTACAGTTTCAGATGCTAAATAATTAGTTAGTTTATAAAATGAAAAAAATTTATCGTCTTCAAAAAAGTTGAGAATTTGACGCTGTCATCAAATTTCAAAAGCAAGTTCTAAATAAATACATGATTATTTATTACATGCCTAACGATGATTTCAAAATTGGTATAACTGTACCGAAAAAGTTTTGCAATGCAGTTTTAAGAAATTACTATAAACGCCAATTAAAATCAATTATCCATTCTTTGAATTTGTATGGCGAAAAAATGCATTTTGTCTTAATTCCAAGAAAAGATTTTTTGAATTTAATATATGAAGATAAATTGACTATAACTAAAAAATTATTTGAAAAGATTAAAAATGGAAAACAAAAATAGATCAGACAAATTCAACTATTTTTCAGATTCTAATTCCGAAAAAGAAAAGAAAAAGAATCTCTGAAAGAAAATATGATACTGAACCAAAATAGTTTTATACACCTTGTTATTTGGTTTAACTATGACAGGATGTATTCAGACTATGGTTATCAAGACCTCAACATATACAGGTGCAGGTACTGAATATTACACATCATACGATAAAATTAGTCCTTATGTAACATCACTTGAACCAGCTAAAGCTGATAAAAAAATCGACAAAGATGACAAGGCTTTACAACCTGGTGAATTCAAGAGAATTGTTAAAAATGATTCTACAAACTACCATGCTAGCTACAAAAATCATGCCGAAATACTTAAAAAGTTAAGAGAGCAAACAGGCGGAGATGTTTATGGAAAATATAACTCATATTCATCTTCTATTCAATGATTCTATGACACTCATAATGCTGATGGTAAATGAAAAGCAAACTATTACGATTCTGCAATAGTTAAAGGTGAAAATGGTAAATATCTTTTTGATATGAGCGAGTCTGCAACATATAAACCTACCTACACAACACCTTATAGTGAAATTAAATTTATTGACCCACAATTCAATTTAAATTCAATTTTATCTTTAGTTGATCAAAAAAATCCAGATCTTTATGAAGTTAAAAAAGATCGTTTAGAAAATAAAACATTCAAAACCCCTAAAAAAGCAACATTGTTTTCAATAGCTACACCAGACCAATTGAAAGCAAAAAATAAAATTGCTGGTAAGGAACTTAGTGAAATTGAGCTTTCAAAACAATACTATGCTCGTGATGTTTTAGAATTTTTATATAGAAAAACATTTATTGAACAAGTAAATGGTAAAGATAATTATTTTGCTAGAAGATTCAAACAAGAGTTTGGTACCGATAAATACAATGATGTATTAGGCAAAATAATTGATCCTACAAATACTAACAAATATAGTAAAAAACAAGTGGAACTTATTTATGAATATCTAAATATATTAAATGGATATCTTAAAACTACAGGTTTCACAAATATTTATGACGACACTCAATTAACAAAAGTTGAATTAGCTAAAGGAACTGAAAAAGTTAAAATAGCTAAAGAAAATGCTTATCTTTCTCCTGCTCCAAAACTTGTATACACTCATAGTGAACCATTCAAACCTTTTGTTAAATGAGGTGATTCATGAAAACGCTGAGGTCCATTCTTTGGATTATTCGTTCACCCTATTGCTTGATTAACAGGATCAATGCGTGAAAATATGCCTTCACTTGCTGGTTGAAGTACAATATTTGTTATTATTATTGCTGTTGTTATAACTCGTCTGCTTGCTTTAGCAATTACTTGAAAATCTCAAGTTAATCAATCTAAACAAGAAGATATTAAAGCTAAAAAAGCAAAAATTGATGCTAAATATGCTGACTTCAAAGGCAATAAACAAATGAAAATAAGACATCAACAAGAAATTGCGGCTTTATATAAAAAGAATAATATAAATCCTCTTGATGCTTTCTTGAATATAATTATTTCCTTCCCTATCTTCATTGCTATGTGAAGGGTTATTCAAAGTATTCCAGAAATGAAATCAACTCATTGACTAGGTATGGACTTTGCTGCAACTAGCTGAAAACGTTTATTTGCTGGTGAATGAGCTTACCTTGGAATATTAGTTGTTGCTTTAGCAGTTCAATTCTTCTCGCAATTTACTCCACGGTTACTGAACAGGAAAAAATTCAAAGAAAGAACAACTATCGAAGAAGAAAAAGCATTGAAGAAAAGTAATAAGACACAAAACATTATTATGATTGTATTCTTATTCATTACTTTAATGTTTAGTTGTGGTGTCCAAGTGTATTGAATCTTCAGTGGGCTTTGAAATATCATCCAAACAATTTCAATTCACTTCTTCAAAAAATCTGCATATTATAGAAGAAAATATGCAAATGCAACCGTTTAAAAATAATCATAAAACCAGATCATTCTGGTTTTATTTTTACTCTTTTTTAAAATAATTTATTAAATATTTTAAGAGTCGACTTTTTAAAATATAATAAAGAAATAAACATAAAAAAAATAGGAGAATAATATGAAAAAAATACATGCTTATGTAGCTGGTCCATTGTTTACTAGAGCAGAAATTGACTTAAGATACGCAATCGAAGAAACAATGAAAAAAGCACTAAAATCAAAAGAATTAAAAGGCAAAATTGACTTTGACATTTTCAATCCGATTCATCTAAATGAAGAATTAGAACAAAACGGAAAATTAACACCACAAGAAATTTTCAAAAATGACTTAGCAGCAATTCAAAAAAGTAAACTTACAATTCTTGACATTGACAACAAAGATGATGGCACCATGGCAGAATTTGGTTACTTCTTAGCAATGAAAGAAAGAGATCCAGAAGTTAAAATATGTGTTTGAATGTCAGATTTTAGAGATGTAGCAGATCGCGACATTAGATTAAACAGATTTATCAACGGAATGATTTATGTATCAGATGGTTGCGTAAAAAATCAACAAGAACTATATGATTGATTAATTAAGGCATATAAATAGAAGGGCTTAGGCTCTTTTTTCTTAATAAAAAAGGCTTTATTTAATTTATAATAGATATATAAAATTTTAGGAGACTTATGAAAAATAATTTAATCGAAAATAAATTAATTGCTTCAATGCGCGGCATAGCATTGGATTCAATCAACAAAGCAAAAGGTGGCCACATTGGTATGGCTATTGGAGCTGCAAATATTACTTATTCATTATTAGGAAAGGAATTAAGATTCTCAAAAGAAGATCCGAAATGAATCAATCGCGATCGCTTTGTTTTATCAGCTGGACATGGTTCAATGTCATATTATTCAATTATGCATTTCTTAGGTTTATTAGCAAAAGAAGACATGCAAAATCATAAAAAAATGGACTCAAAAACACCTTCTCATCCTGAGTTAGAATATACACCTTTTGTGGATGCTTCAACAGGTCCTTTAGGTCAAGGAATAGCTATGGCTACTGGTATGGCTCTTAGTCTAAAAGTACTACAAAACAGGTTTAATCGTAAAAATTACAATATTATAGACCACTACATTTATGCGCTTCATGGAGACGGTTGTATTCAAGAAGGTGTGGCTCATGAAGCTATTCAATTTGCAGGTACAAATCAATTAGATAGACTTATTTTAATTCATGATTTTAATAATGCTCAAATTGATACAAAAACCGAGGTTGTTAATAATATTAACTTCATTAAATATTTTGAAGCTTGTCATTTTGCAACCTTTGTAGTTAAAGAAGATAAACCTGAATTAATTCTTGAAGCAATTCAAAAAGCTAAAGCTTCTAAGAAACCTTCATACATTCAAGTTCACACAGCAATAGCTAAAAATACACCTTTTGAAAACTCGCCAAAAGGTCACCATGGAATGTTAAAAGAAGAAGATACTATTGAATTTAAAAAGAAATGTGGTTTAACTTCATTTGTTCCTTTTGAATATGACAAAGAAGTTTATGACTATGGAGAAGAATTATTAAAAGAAAAAGACAAACATTATAAAGAATGATTACATCTTTATGATAAATATAAAAAAGAATATTCTCAAGAAGCTAAAGAATTAGAAGATTTATATAATAAAAAAGAAAAATACAATTTTGATGATATTGAATTTAATGAAAGTAATGCAGCAACTAGAAACTATTTTGCTCCTATAATGCAAAAAATAGATCAAAATTACAAAAACGTTTTAGGTGGTTCAGCTGACTTAGCCTCAGCTACAAAAGTTAACTTTAGCAAATCTTATGCTGAAGGTGGCAAAAACATTCACTATGGAATTAGAGAATTTGCTATGACTGCTATAAATAATGGAATCTATTTACACTCACATTTAAGAACAATAGATTCAACATTCTTAGCTTTTAGCGATTATGCTAAAGGTGCTCTAAGACTAGGTTCAATTATGAAAATACCTTCAGTTCATGTTTACACTCATGATAGTTACCAAGTTGGGGGTGACGGACCAACTCATCAACCAGTAGAACAATTAGCAATGCTTAGAAGTACTCCAAATTTTAAAGTTATTAGACCTATGGATGAGAGCGAAGTCAAAGCAGCATTCAATTATGCATTAAATCAAAATGAAAATCAAGTAGCTATTATTGGTTGTCGTCAAAATGTTAAATCTTACAATTTACATCCTGAAGGTGAATTGCAACCAGCTTACATTATTCGTCCTGCAAAAAACCATGACATTAGTATTTTGGCTTCTGGTAGTGAAGTTGACTTAGCAGAAAAGGTTGCTAATAAATTAGTTGGAGCAGGCATTAAAGCTCAAGTTATTTCCGTTCCTTTATTACAAGATTTAGTTAATGATGAAAAATTAATCAAAAGCTTAGGCATTGACAAAAAACCAATATATGCTATTGAAGCTTCAACAGATCCATTGTGATATAAACTTGGAAAATATAACAAAATTGATGCCTTTTTAGCTGATGGCTATGGTTATTCAGCTGATGGTTTTGAAGTGTACAAAATCAAAGGATTTAGCTTAGATAATATTCACAAAAAAGTTTTAGAGTTCTTAAAATAAAAAGAATAAACACAAAAAAAATATTTAAGAGATTTCTTTATTTATTTAACATGGATACATATAATTTTTTTCACTAATAAATTTTAGATACGGCTTATTTATAGGTAAATTTTTCAAAACTTAGTTGTTTTGATTTTGTATTGATATATAATAAATTAACACAAAAAAAATATAAAAATAAAGGGAGAAAAAATGACATCTACAAAATCAAGAACAGTTTTAACTATTCTTTCATTCTTCCTTGGATGATGTGGAGTTGATAGATTCTACTCTGGTGAAGTGGTTCTTGGATTTTTAAAATTGGTAACAGTTGGTGGATTAGGAGTATGATATCTTGTCGACTTTATTCTAGCTATTACTGGCGCACAAATAGATGGTCAAGGATACATTATTAGCGATTGAAGTCTTTCATCTACACCTGTAAACGGAGGACCAAGAATCGAAACTGATGAAGGTCCAAAAGACAAACACAGCTCATAATTTCAAGCTATTAATGAACTTTATTCTATTAATTGGAATAGAGTTCATTTTTTATTCGAAAGTTATTAATCTTATATTTAATGCTTTCTTTAATTTTTAATTTTTTTGAATAAATTGAATTATATTCAAACATATATAGTGTTATGTAAAAAAAATAAATGATTTTTCTGTCATTTTTAACCATTTTTTGCAAAAATTGTAATAAAATTATAAAGTAACATAATAGGAGGAATAAATGTCAGCAAAATTTTATCAAGTTATAGAAAATCAAGGGATTGGCACACAAAATTGATTAATTTGAAAACATCCAGAGTGTGAAGTAAATAACAATTCAAAATTAATCGTTAGACCAGGTCAAGTTGCCATAATTGTTCAAAGCGGACAAATGATTAAGCTTGTTGAACCAGGAACAAGTAATATTGATAGCGATTGATTCCCTATATTAAAAAATAAGACAAAAGAATTTTGAAATAAAAACCCATATCCAATTGAATTTTATTTCGTTAACACAAGATTAAAATTAGACTTTCTTTGAGGAACAAATTCGCCTATTCCACTTAGAGACCCTAAATATGGATTGCTTTTATATTTAAGAGCAAGAGGTCAATTTGGTTTAAGAATTACTAAATATGAATACTTATATAGAACATTAAATGGATCATTTGATTCAAGTAGAACAATTTATTACGAATTCATAGACAGAATCTTAAGAGGCTGAATTAATCAAAACATTAAAAAAATTATTTCAAAATTCATTCTTGAACACAAAATACCTTTTGATGAAATAATCGCATCAATAGATAATATTAATGAAGATTTCAAAGAAGCTTTAAAATCTGAAACACAAAAATTAGGTATGGAAGTTGTTAACTCAAGCATTGAAGATATTAGTATACCTAAAGAGCAACAAGATGAATTGAACAAAATTCTTAAAAGAAAAGCCGAAGTTGATATCATGGGAGACGACTATGCTAAAGTTAGAGGCTATGACGTTTACCAAGATGCAGCAAACAATTCGGGTTCAGTAGGAACTTTTTTTGGAGCAGGCATTGGTTTAGGACTAAACGAAGGTGCTTCAAATTTATTAAGTGATAGCAAAATCAATAAATCAGCAACCCCAACAGCAGCAGCTGCATTATTAATCACTTGCGGTACATGTGGAAGCAAAGTATCTAAAGATTCTAAATTTTGTTTTGAATGTGGTTCAAAAATCGCAACAAACAAATGTTCATCATGTAATATCGACTTATCGCCAAATGCAAAATTCTGCGTAAATTGCGGAAAGAAGGTAAAATAAAATGAATAACAACAATCAACAACAAGAATTAGTTGAACCTAAAACAACAATCTTTAATGATCGTGAAACTATTAAAAAAAGATGACCATGAATGCTCGCTTTTGGATTAATATTCATCACAATGATTTTAATTCCAATTGGTGTTAAGAAATTTCCATTTCATGACAAAAGCACCGACATGTTGTATTGATATATTGGTGTTAAATGCTTCGCTTTATTGTTTTTAATTTTCGGTTTTGTACACACAACATTCTTCAAAATAGCTAGATCATTCTACTATAGATTAATGGTAATAGTGGCCCTTTATCAAGTTGTGCCATTTATATTAAGATTTTTTGGTTACTTACCTAATTTCAACAATATCAACATTTGATTATCACTTTACTTTGTTGTATCACTTATTATCTTTATCGTTCCATATGCTACTTTAACATTTATAAGCAACAAAATGAGTAAAACAGATCAAAAATTTAAATCAAGAGAAAGATCACTTAGAGAAGAATACATCACAGGACAAAAACCTAAAGATGAAGAAGGGTTGTACTAATTATGGAATGCAAATCATGTGGAGCTAATGTTAGAATCACAGATAAATTTTGTTCTTTTTGCCGATCATTAATCGAAGCACCTAAATCAAGCGTTGAAACATCTCAACACACAACTGCAACTACAAGAGTAGTAACAGAATCAAAAGCAATTAATTATGATGTATTGAACTTATTAGTTATAGAAGATAAATATGACGAAATTGATTTTGATTTATCTTCTAACACAGCTGCAAACTCAATGAAAACATATGTAAACAAAGGAGGAAGCATTTAAGAACTTGAGAAAAGTTATGTTTTAGCAAATAAAATCGATTACTATGAATCACTTGACAAAGATGAAATCTTAAAAACTTACTCAGAAAAAATTAAGAAATATTCCGGTAAAGATATTTGTTGTTTAAAAAGAAGTGAATTACAAGCCTTAGAAAGAAAAATGAGTTTATATGAATTCAATGATTATCTTAAAGTATTAAAAGATAGAGTATTTAATAAAGAAGGAATTATGCTAAGTGACAGCGACCTACTCGACAAAATAAACTCAGATAGAAACATTGAAGTTTTTGCTATTTGTTCTTATGACTTCTGAGACAAAAAAACTGTTGAATTAGATAGACAGAACAAAGTCGAATATGAACAAAGAATAAAAAGAATTAATAACAAAAAGAAAACAGCTAAAGTCATTATATGAACTATTGTTGGTATTTTTATTTCTATATTTATAATATGTTTTTTAGTTGCCTTAGGTGTAACAATTAGATCTTCTGTATCAATTGCTTCAACATCAAGCAGTTATTACTAAAAACAAATTGACATCTTTTTAAAAGGTGTCTTTTTTTATTAACTCCAAAGCAAAAAATAAAACCTAAACTAGTACAAAAAGACTTAAATATTTTTGGGATTTAAGTCTAATTTTTTTGTCCTGGTTTATTATTAAACTCAATTTAGTTTACTATCATGTAATAGTTGATCTAAATAAAGCAGGGAAGAATTTAGACATAATTTTTTTACCATAAATTACCTCCATAATTCATTGTCTTAATGCATCATGTTTTTCTACTTCATTTTCAAAAGACAAGTGTTCAAGTTTGATTTTTTTATCAATAAATCTTGATACTAAATTGATTTCATTCATTTTAATTGCTTCTAATTCTAAGTTTATTTTTGAAATATAAGCTATCTTTTTTATGTCTGTTTCATAGTCATTATTATTAACTTTATTTCATCTTTCAATAGCATGGTCTGAAACCATAATATCAAAAGTTTCTATTGCTTTATCTCTTATTTCTCTAAAGAATTTTGCATAAACATCATGCTTATATCATCTTTTAATTTCATTTAATTCTTCTTTTGCTAAATTTTTTGAACCGCCCGGATTATATTCCATTGCATATTCTTCTAATTTATTAATTGGTAAAGGATCAAAAGCTGGAATATAGTCTTTAGGAACTAAATTAATTTTAGCAATTTCATTTGGTTTGAATGTTTCCATGATAACTTGGTAGTCACAATTATCTAAGGTTAAAGTTTCTTCTTTAACAATTACATCAAAGTAAATAAATGCTCCATATTTCTTTTCTAATTCAATTTTTTGTTGAACAATTTCATTAAATTTTTCATTTAAAACTGTTTTGCCACTTAGAGCAATTAGCATAGGTTTTGAAATTGGTTTTGAGTGTTTAATTTGTTCATTCGTTGTTAATTTAATTCAATCAAATTGAGGATAATTTTTATCCAAAGTTGTAATACTTGATTTTACTAAAGCTTTCATATTAACTCCTTTTATAATTAACTCAATTTCTTATTAAATATAGCATAAAACAATGGTTTTGGTAAAAAATTAATTTGTTTAATTCAAATATTTTTTAACATTTTTATATGACTTTCTATGAAATTCACAGATGCCATATTGACTCATTGCTGCTTCATGTTCTTTTGTACAATAGCCCTTATTTTTCTTGAAATTATACATAGGAAATTTTTTGTCAATTCCAACCATAATTAAGTCCCTAGTTACTTTAGCTAAAATTGAAGCTGCTGCCACATTAATACTTAATTGGTCACCTTTTACTAAATTAATTTGAGGTATATTGATGTCATTAATTTTTTCAAAATCTGTAATTACTAATTCAGGTTTTGTATTAAGTTTATTAATACACCTTTGCATGCCAATTCTTGAAGCTTGCTTTGGATTTAGTTGATCTACTTCTTGAGCATTAATAATAGTTGTTACTGTTTCAATAGCATTTTCAAGAATAATTTCTAACGCTTCCTCACGTTGTTTTTGACTCAATTGTTTAGAATCCTTTATTAAAGGATGGTTAAAATTTTGAGGCATAATAACACAACCAACTACTAAAGGCCCTGCACAACAACCTCTTCCTGCTTCATCTAAGCCTACAATTAATTTAGTGTCAGGATAATTTTCTTTTTCATAATTCAACATAATCAAAATTATACAAAAAAAGCAAAACTTGAATTCTGCTTTTTGTATTGAAATTATTTTTTGTCTTTGTTTGCTTCTTCGTCTTCTTTAGCCTTTTGAGTATCAACAATTTGAACAATTAAGCGGCATAATTCTTCTTTGTCCATTTTTTTGTAACCACTAATGTATAGGGTTTCTGCTATGTTGTAAAGTTTTGCATTAGGCAATGCTAATAATTTTTTGATTTTTTCTTCATTTTCTTTTTCATTATTTTTAAGATGTGCTTTGTTATCAACTATTTCAGCTTCTTCAATATCTTCTTCTTTATTGAATTTGCTTTTATCTTGATTAGCACTTACATCATCAACATCATCTAACATGATTCCAAAAAGATTGTTTGCTAAATTGCTTTCATCCATCATACCTGATTCTTTCATCTTTTTTTGAAGTTGTTTGATTTCTTCTAATCTTCTTGCATTAATAAATACACCTTTGAAGTATCTTAATTGACTAACAAACAAGAATTGGAAAACAATTAAAACAACTGTGATAACTGAAGTAACTATTGTTATTGATAAAATAATTGGATTGTAAGCAGTAGCTAGTGATCTAATGTTTCTAATAATTGAGAACAAGTTTATAAATCAAGAAATGATTAAAACCATTGAAGGTCATGTATGCATGTTGGCTAATGATTTTTCTTTAAATGTTCGAGCATAAAAAACTAAAAATACAATAATGCTAACTAACATACCAACTGTAACTAAACCATTTGTAATAATCATGCTCATTATTTGATCTTCAGGTTTAAAACTTGGAGTTGTACCACCATTAATTTTTACAATATAGTCAACTAAATTATCTTTAAAAGCAGCGAGCAAAATTGTTGAAGTTAAAGCACAAGCAAGTGCTAAAAAGAATGTTAAAAGAGAAAGAATTACAACTGGTCTAAATCTTTCTTTTTCTAGAGCTCAAAGTTTATTTGTGCTTTTAATTTGTAAATATTTTTTGTCGAATAAATTCATTTTTTCTCCTATATCTAATTAATATATTATAAAACATTATTTTTTAATAGTTCATTAAAATCATAATCGGCTACATCAAATTCTTTAGGCACCGGTGCATAAACACAAATTTCTTTTTTAGTAAGTGGATGAATAAATTTTAATTTATAAGCATGTAATCTTTGATTAAATTCATCAACACTTTTGCCATAAGTAGGATCACCGTAAACTGGATGTTTGATGTAAGACAAGTGGACTCTAATTTGATGAGTTCGACCTGTTTTTAATTCGCATCTAACTAAAGACATTGGTTCTTTATCTAAATAAAAGTGCTTAAGCAAATGAACAAAGGTTATAGCTTCTTTACCTTCTTTATGAACTGACATCATTTTACGATCAACAGTATTTCTAGCAATTGGTAAATTAATTTTAGTAAGTGGATTTTCAATTAAGCCTCTAACGATAGCTAAATAACTTCTTTCAATTTCATGTCTTTTGAGCATGTCAGTTAATGAGTTATGCATCTTGTTATTTTTAGCAATTATTAATAAGCCACTTGTGTCTTTATCAATACGGTGTACTATACCTGGTCGAAGTAACCCATTTTCATTTGATAAATTGTTTTTAAAATGATATAAAAGTCCATTAACTAGTGTGTCATTGTAATGCCCCGGTGAAGGGTGGACAACTAGATTACTTGGTTTATTAATTATTACTAAATCATCATCTTCATATTTAATGTCAAGAGGAATATTAACTGGTTCAATTTTAATTTCTTTGTTAAGTAATTTAACAATTGTAATTTCTTGTCCTTCGCGAACGATGTAATTACATTTATGTACTTGAACGTTGTCATTAATATAAACTGCACCTTGCTCAATTAACTCTTTAATATCATTTCGACTTAATTCACTATTTTGACCGATATACTTATCGATTCTCTCTTTATATTGAACACTAATTTTTAACATAGAGTAATTATATATTATAATTAAAAATTCAAAATTGTTTTTAACCTAGTCAAGGTTCAAAAATAATTAAAGCTAAAACTATGTTGCTTAAGATCAAAGCGACCACAGGGACTTTTTTAAGATAAAGAGTAGTTGAAAGTATCACTCCAACAGGCACATAACAGTAAACAGCAATTCGACGTCAACCCCTATAAAACAGTGCTTTTTGTGTATCATTATTTATTCCTACATAGTTAGTAAATGATTTATTAAAGAAAACTTGAGGTGCTACTGAAGCTATAAAAAGTTGAAAACCCAAGGCAACAATAATACCTGTTACAACAGGATTCATTATGTTAATTAATTTTATTAAATATTTATTATTTTCAAATTTTTTTGAATATTTCATTGCTAATTTCATCATTAAAATTGGTGGAATAACAAAGACTAAATAAGTTAAAAACATAACTATAAATCCTCATCATTCTTTATTAGCATAGAAGTATCCCGTAACTAAAGCAAACTTAGTAGATAAAATTCCAGGTGTTGCATTACCAACAGCAAATACTTGGCTAATATCACTTTCAGTAATATTACTTCCAAATCATTCTCTAAATAATCCTCAGAATCAACTAAAAATAGGCATAAATATTTGCCCACCGCCAAAAACAGATAAGCTAATTAATACCAAAAAAGGTAGGGCAACAAGTAAAGCAAGTCAAGCCATTATTGACCTCCTTTCTTGTCACTATTCAAATCATTTTCTTTGGTCTCTAATTCTAAGTTTTGTTCTATTTTTTCTTCTATGTTATTGTCTAAATTAGTAACTTTATTTTCAATATTTTCTTTAAGTTTTTTGATTTCTTTTTTGTGTTTGATTGCTTCAACAGTTGCAAAAATACAAATAATTAGAACCATAATTATTACAGGCATATTTCATGGAGTTGGTATAAAGAAACTAAAGGCAAAGGTTATTAAAAATAAAATTAATCACAAACTAATTTTAGTTTTGCCTCTTGATTTTTTGAAGTATTCCCAAGCAAAACCAATCAATGATCCAATAATTGCAACAATAACACCAAGTTCAATAACAAACAAATATTTTTGAGGAATATGTTGCACAAAATAAAAGATTAAAAAGAAAACTAAAACATGAGGTAAGGTTGCAAAAGTTGCCAAAAACAAGCCCTTTTTGAAACCTAAAAGTTTAATAGTAATATAACTAATTGCTTCAATGACGCTAGGACCTGGAAGCATATTTGTAATAATTACATTTTCATCAAATTCTTTTTCATTTAATCATTTATATTTATCAACAGCATACTTTTTAATAACAGGCATCAAGGCATTGCCACCCCCAAAACCTATGAAAGTAATAAATAAAATAAACAAAAAAACATTTCAAAATGTTGGTTTTTTTGTATATTGAGTTGCTTGTTGATTTTGATTATTCATATTTTGAATTATACTTTTATTTTTAAATTTTTTTAGTGCTTTTTTTGTGTACAAAAAATTTAATTTAAATAAGATCAAAATTTAAAATAACTTACAAATTTAATATAATTTTTATAACAAAATTATTTATTTATAATATTCTTAGACAAACAGGAGATATTATGAATGCAAAAAATGTTTATCAATTGCAAAGAGAAAAAATATTAAAAAATGCTTTTATTGACAAAAAATTGAGAAAAGAAAATATCGAAAAACTTTACTCAATAATTGTGCAAAATGAAAGTAAAATTAAAGAAGCTTTATCTTTGGACCTTAATAAATCATCACTAGAATCATATTTTAGTGAGATAGGTTTAGTCTTAAAAGAAATCAAATTTAATATTAAAAATTTAAATAAATGAACTAAGCCTTTTCATGTTTCAACGCCTTCTCATTTAGCTTTAGGAAAAAGTGAAATTCAATATGAACCTTTAGGTCAAGTATTAATAATTAGTCCTTGAAATTATCCTTTTTATTTGAGTTTAATGCCATTAGTTTCAGCTCTTGCAGCAGGCAATAGAGTAATCATTAAACCAAGTGAATTTTCAGTCCACACTTCACAATTATTACAAGAATTAATCAACAATAATTTTGATCCTCAATATGCTTTTGTAGCAGACAGTTCTTTAGAAGTTGCTCAAGAATTGTTGGACTACAAATTTGATTTAATTCATTTCACAGGTAGCACAACAGTTGGAAAAATTATTGCTAAAAAAGCATCTGAAACTTTGACACCAATTACTTTAGAATTAGGTGGGAAGTGCCATTGCGTTGTTGACAAAAGTCAAGACATAATTTATTCTGCTCAAAAAATTATGGCTGGTAAATTGCTTAATGCAGGTCAAACTTGTATAGCTCCTGATTATGTTTTAATTGATAAAAATAGAATTAACGCATTTAAAGCTGCTTGTTATGTTTATCTTGAAAACGTTTTGCAAAATGAAGAATATATTAAAGGTAATTATCCAAAGATAATTAATCAAAAACATTTTGAAAGATTAAAAAAATATTTACCTAAAGATCATAAAATTAATGAAAAAACATTACAAATAATGCCTTATATTTTTGAAACAAGCTGAAATGATCTTATAATGCAAGAAGAAATTTTCGGGCCGTTGTTGCCTATTATTCCTTATGATAATTTAGATGATTTATTAGTCAAATTTAAATCACTTCCTAAACCTTTAGCTGCTTATTTATTTACTAAAGATAAGAAAATAATAAAGCTATTTAAAGAACAAGTTACTAGTGGAGCTTTAGTAATTAATGATACCATTATGCATATAGCAAATAGTAATTTACCTTTTGGTGGTGTTGGTGATTCTGGCTATGGCAGAAGTCATGGTTTTGAAGGTTTTAAAGCTATGTCAAATCCAAAAGCTATTTACCAAAGAAAAGGTTTTAATTTTAAATTAAATGAGCATCCTTACAGCAAGAAAAAAGAATTCTGAATCAAAAAAATAATTAAGTAGATTTTACTTGGAATACTATAAAACAGCATAATAAATCACACTTAGTGTAAAAAATCAGTGTGATGTTTTTTAAAATTTTAGTTAGTAATCTATTTAAAGTTCTTTTAAATATTGTTTAAATATTAATAAAGATTCTTTGAAACTTTGTAGATAATCAAAAAAGAAAATAATGCCAAAAATCAAACTAATAGTTATGCAAATAACGTTAATTATTTCAAAGCTTATTAAGAAATAAATAAGGAAATTTGCTTTAGTTGAAACTGCTACTTGCGAAATAGTTAAGATAATTAAGATTACTCCTAACGATATATTGACATGCGAAAAATATTTTAATTTTTCATGACTGTAGCCAACAATAATATAAGGCAACATTACTAATATTGTTTGACCAATTAAAGATATAAAAACTCCACTACCTTTTTGTCAACAACAAACTAATACAACATTAATTATTAATAAAACTAAGCCAATTATTACATAGCTTAATAGCATTCATCATCTCTTTTGTTTATTCATTTTTACTCCTTAATAATTATATTTAAAATTTTTATTTTTATAGCCTTGTATGTTCAAGTTATAAAAAAATTACGTAAAAATTAAATTATAATTTTAGAAGGAGATAAACAATTATGAAAATAGCAACAGCAGATATTGGCGGAACAAATACAAGATTCGCTTTATTTAAAAAAAATAAATTTTGATCTAAAGTTAAATTTGAAACTAATAAAAATAATTATCGTGAAACTTTAAATCATATTGCTCATTTATGCAATTTGCATAAAATAGAATGCTTGGCTTTATGTATTCCAGGACCAGCCGACTATGACAAAGGAATAGTTTTAAATACACCTAATTTACCTGGTTGAGTTAACAAAAACATTAAAAAATATTTATTTAAGCATACTAATCTAAAAGAAATAATTACTGAAAACGATGCTAATGTTATGGCTTTGGCAAATCACAAATATTACAAACAAAAGAAAAGTGATGTAACTCAATTTTTCACAGTATCAACAGGTTTAGGTGCTGGTCTTATTATTAATGATCAAATATATGTTGGTAAAAATCACTTAGCTCAAGAAATCGCGAGAGCACCTTTAGGTACTGATGAAGATGACTCATTTCATTTACTTCCTTATTCAGTGGAATTGTATGCTTCAGGAACAGGCATTGAATTAAGATATAACAAATTAACAGGAGAAAAATTAAACGCTAAAAAAATTTTTGCTTTATATCCAAATAATGAAAATGCAAAAAAAATAATAAATGAAGGTATAGATTCTTTAGCTAGAACTATCTCAACTTCTTTAGCTTTTTTAAATCCAAGTCTTGTTGTTTTTGGCGGTAGTGTTAGTGAACATAATAATTGGTTTATAGAAAAAGCTATTGAAAAAGCTAAAACATATACAATTAAATCTCAATATCAAAATGTAAAATTTGCTTTTGATCCTTATACAGATTTCAGCGCTGTAATGGGTTTATATTGTTTAGCAAAAGATAAATTTAAATAACATTTTTTTCTAAAAATACTTTAAAATAGAATTATTGGTAAATCAATAATTTTTTTAAATCATAATTAATGAAATTTTTACTATTAATTTGTATAATTTTAAAGTAATCATTAAATTAAAAATGATGTAGAGAAGAGATGAATTAAATGAAAAAATTCAAGTGACTTTTAGTTCCATCAGTTATTGGTGTTTTATCTACACCAATGATTTCAGTAAGTTGTTTAGGAAATCAAGAAAAATGAATAGCTTTACAAGACTTTGTTGACAATTTAAAAGAAGGAAAAGATTATAATTTTTCAATTTCTACTGACGACTTAAAAAAAGAATGCAAAGCTATAGCTGATGAATGAACAAATAATCCAGAATTCAAGAAGAAATATGCAAAAGATGTACTACCTCCTTCTACAAGACAATTAATTGATAATCCTTCTGCAACAATAAATCCTGAACCATCAGAATTTACTGCTGAATATTTATGAATAAGAGGATATATAAAAATTAAATCTAAAAATCCTAAGTATAGATTCGATGTTCTGGAAGTTTATGACAATGGAACATTAAGAAATGCTAGAGCTAGGGTTGGTTTTAAAATTGTCGATGTTTCTGATGAAAATTATTATCAAAAGATTAATACTAATAGCTCGCCTATTAATATAACAAATACAAGAAGTTATGGTAGTGAGCACTTCCATGTTGATTTAACTAAAATAAGAGAAAATGAAAGAACATTATTGAAATTATATGCATTTAATCTAAAATTAAATGGTTTTTATGAGTATGACAAACATTAGCAGAAAGAAGAAGATGAAAAAATATTTTTTGAATAGATTTATTTTAGGTAGTGCTTTATCTTTTGCTCCAATAATATCTGCTTCTTGTGTTCAAAAACAAGAAGTTCCTTTTGAAGACAAATTAATTGAATCAAAATATGAAAATAGTTCAATAATTGGCTTAAAGGACAAAGATTTACTTAAATTAAATTTAAATCAAATAAAGTATTTTATCGAGCAAACTAATAAATTAAGATTAAAAAATAATTTTGAACTAAAACAAGTTGTAAAAGAAAATGATAATCTATTTTTGTCAATAAATAATAAAAAATATTCATTAAAAAGTTTTTTAAAATCAGTTAATGATAATTGGGATATATTATCAAAAAATTGAGAAATTGTTACAAATAAAAATAATAGCCTTATTAGAAAAGGCAACAATAGTACTGACGTAAATGTGTTTTTTGAAAATGATAATTCAAATTTTAAAAATTTAGGCTATGTTAATTATGTTAAAAGATTGTTTAAATCAAAATACAACAATTTAGCAAATGCTTCTAAAAATTTACCTGATTTACAAATGATTTTACAAGTTGCAATTTCAGGAAGTAATAGATTTTCAATTTGAAAAAATCAACTAAGTTCAAACTTATTAAGAATTGAAAATATTTTTGATGAAAATGCTATTTTAAATCAATTCAAAGAATACTTCGAAAAACAAGCAACTTTTTACTTAAAAGCCTATAAAATAGTTAATAAAGAAAATAAAGAATTTGAAAAATTAATTATTACAAGTAAAGAAAAAGAAGAGTACAAAGGCAAGAATATTTTAAAAATAAAAGTAGATTTATTAGATAAAAATAATCAAAGTATATTAAATAATGATCAAAAAAATAAAGTCTTTTATTTGATGGATTTTCAAGATTCTAATCCTAGATATCATGCATTTCAAAAATATGGAAATTTTGAAATCGATTCTTCATTAGAAACAAATGAAAAAAGCAAATCTTTATTTAATGAAATGTTTAAGGAACCAACACTTTCATTTGAAAAAAATATGTTCAATTTTAATGATTTTGATTCATTAATGCACCCTTCAAAACCTTATTTAGGTTTTAATCTTAATTCTTTTATGCAATTCTATAACGCTTATAAAGAAAAGGTCAAAATTAATGTTCCTGATGAAAATTATGAAGCAAAAATAAAAGAAATAAAATTTGACAAAATATTAAATAATTCACATTCAATAGGCAAGGCTATAGTTGAAATTAAAAACAAAAAAACTAATAGAGTACAAGAAGCTAATTGGTTTACTATTGACTTTACATATGCACATAAACATATATTTGATGGCTTTTATATTCAAAATAAATTAGATTGTGATCCTTTAAAAAATGATGAATTCTTCAGCTATTATATAAATAAAGGAATTGGAACAAATAATGAATTAAAATTACCTGAAGGCATTAATGCAGAAGAATTTTATAACACTAATTTAATAGATATTGTTAACTTTATTATTTATCAAAACAGAGATTCATTTAACATTTGAAATAATAAAAAAATGAACAAAAATAGTGTTCTAGAAATAATTCAAAATAAAGCAGAATTTGAAAAAAAAATAAGTACATTAATTTCCCAATATGTCCTTAAATTCTTTATAAACAACAAAAAACCAGGTTATGAAGAATTAATAAAAGAAGTTAAAGTTACTATTGATCCAAATAATGATTATGACGCCTTAAAATATGGCGTCGGTAAGTTACCAATTAGAATAGATTTCATTAATCATAAAAATGAAGATATGATTAAAAACATAAGTGAAAGAAAATTCTTATTAGGCGGTTTTGTTGGTTATGATTTCAGTGATATTGAAGCAAAATTAAAAACATTAACTGATGCTGATTTTGAGAGACCTCTTAAGGATAAAACTCCACCTTATTTAATCAAAAACTAAACAAAAAAACTTGCGATTGGCAAGTTTTTTTCATCTGGCCGGTAGTAAGGGATTTGAACCCTTGAATGACTTTCACCATTATTGGTTTTCGAGACCAACCTCTTCAGCCTCTCGAGCAACTACCGTTAAAAAAATTATAAATAAAAACTCAAATTAATGAGTAAATTATTTATGAACTTTATGATCATGAGATGCGTGTTCGTTTGTGTGTTTTAATTCTTTTTCTTTTTGAATTGTTTCATCAAAACGATGTAATAAATCGTGTTTCATTTCTTTTTCTTCTTCTTTAATTCTTTCTTTAACTCTTCTTTCAATTTCTGCATCAATTATTTCAGCAAGCTCATTAGCTTTTTCTTTATTATCTTTTTTCTTTTGAAAACTAGTTAAAACACTTGCTGCTACCATACCACCAGGTATAGCAATAATTGCAATACCAACCATAGAAATTAAGGGCACAATCATTTTTGTAAAACCACTATAAGGAATATAGTCTCCATAACCAATAGTTGTTAAGGTTATGGCACTAAAGTATAAGGCATCTCCAAAGTTTTTTACAATGTTGGTGTGTTCTGTATAAAACTTAATTTTTTCACTTTCAAATTTAGTTGGATTAGCTTTAACTTCAGGTAATTCTCTAACTAAATTATTTACATATTCTGTTTCTGTATATCAAACTGTAAGAGCAAATAAAACGATCAAAATACCTATAAATAAGAAGATATTGAAAAACAAAACTTTTTCATTTGATACAGATTCTGAAATATTTTTAAAAACTACAAAAACATTTAAAAGCATAATTAATCTTAAAATTCTTAAAAACTTAAAGCCGTTAAAAGCTTTTAATGCATTACTTTGATTAGCTTCAGGCAAGAAATTATTAATCACATAAAGTGAAGGCAAAAATGTAAGAATCAAAATAATATTGCCGCTTGTAATCAAATATTTTAAATATGAAACTCCTATATTTTTACTTTTCTTGTCTCTTGCTGGATAAGTGATTCAATGTATTAATCAGTCAACAACAAAAACAAAAAATGAAGAAATTTCAATAAATTTAATTGCTTTATCAAAAGTATTTTTATGATTTGAATACTTTAATAAAATAAAGCTAACAAATCCAAATAGAACAAAAAGTGCAATGAAAACTAAATAAAGTGTTCTAAAAATTCATAATGTAATTCTATGTTTTTTGTTGTTTGTTTTTAAATCGTTATCAACTGTAACAATTATTCCAATTGCTTCAAAAATGTCATTTATTTTCTTATTTTTTATTAAGTTAGTTTCTCTTTCCATAATGGAAAAAATTATAAAACTAAAATCAATATTTTGTTTATAATTAATATACTTATTTTACTTATACATTTATTTTATAGGAGGTTATATGAAGGGTGGCGTAACAATGTGGATAATACTTGGTATCTTAGTTGCTGTTTTAGCCAGCTTCTTTGTATGAAGTGCTGTTAAAGAAAAGATCAAGAAAAAGAAAAGAAAGAAAGAAGAATTACAATTCAAAAAAGATACTGAACAACAATCTAGATTACTTATTTTTGCTTTAAAAATATTAGTTGACAAAAATGAAAAATATTTAAAAGAATTTGAACCATCAATTGGTCAATACAAAATGTCAAACATAATTAATACAGCTCGTGGTTTCTTAGAAAAATATCAAGAAGATGCTTTATTTAAAGAATGTATTTTGAATAATGCTGATGCTAGGGATGAAATTTTTGCTTTTGGTTTTTTAAGAGATACAAGAAGCAATTCGTGAAGTAAAAGATGTGCTGATAAACTTCAATGAATAGAAGAAAAATTTAATTCATATAACATTTCTCTTTATGAAGATGAATATCAAAGAGTGGAAAAAAGAGTTAATGATTATTATGATGCGGAGTTTTTAAAAGATGACAAAGAAGATGAATCTACCAAATAAACTAACAATACTTAGATTGATTTTATTCTTACCTCTTTTAGCTCTTTTTATTACTTATAAGATGATCCAAAATTACATGGATAATTACTATAAAATAGCTGGCCGTTCAATATTATCTGGAATTTTACTTATCTTTTTAGGAGCAATGATTACTGACTATTTTGATGGCAAAATTGCTAGAAAATACAATATGATTACAAGCTTTGGAAAGCTGTGAGATCCTTTAGCTGACAAAATTATTGTCACAAGTACATTAATTTTTTTAGCTGCTTTTAAATTCATTCCTTTATGAATTGTTATAGTTTTTGTAGTTCGTGATTTGATAGTGGATGGTTCTCGTGTAATAATGGCTGAAAAGAAAATTGGGGTTGAGGCTTCAATTTGAGGAAAGATGAAAACCTTGTTTCAAACGCTTGCAATTATTGCTATTTTAATTATTGCTATAAGCTTTGAATATCCTATTGAATTGGTTGAAAAATATGGCCTAACTTTAGTTCAAAGTGATGCTTGTATTTATGCAATTAACACTCTTACAATTGTTGCATTATTCTTCAGTATTTACTCAGGAATATTGTATGTTAAAAAAATAATCCCTTTCCTAGAAATGGTATAAAAAAGCTGCTTTGGTCAAAAAGCAACTTTTTTATATAATTATTTTTTATGTAAGTAAATAATTAGTGCAAGTATCAAAATTACTGTAATTGCAATGATTATTCCAGCTACTAAAATTACAGCTAAAGAAGACAAATATCTTTTCTTCCTATTTGCAATTATATTGGCTTCATTTTTTTTAGCATTATCAATTGCATCTTTAACTAATTTTTCTCTTTGTTCATTAGTTAAATTGTTTAAATCTTCATTCTTAATAATTCTAAATTTTTCAGCATCGTATAGAGTTGTAATCTCTAAGCTGTTTTCTTTTTTGTCATCTTTATTCATGATAATTTTATTTTATCAAAATTGTTTCATTTAACTATGAATTAGCTAAATTTTCAAAAACATCCTTCATATTTTCATTAAACAACAAGATATTTTCATTGTTACTATTTTCATAACGTGAAGTATCTTTATTTAGAATTACTATTCTTTTACCCTGATAAAAATGAATATATGAAGCTGCAGGATAAACACTTAATGAAGTACCTGCAATAATTAATAAATCACTATTTGAAATGGCTTCAGCTGCATCATTAGTTGAATCTTCCATTAGTGGATCTTCATATAAAACTACATCTGGATTTATTACTCCCCCACATTTACAAGTAGGAATTCCATCTTTATCTTTTTGATGAATTAATTGAGCTAAATCATAAAACTTACCACAGAGCAAACAATAATTACGATTAATATTTCCATGTAATTCAATTACATATTTATTACCAGCTTTAATATCCAAGTTATCAATATTTTGTGTAATAACATGGCATTTATTTTTATTGCATCAGCTTGCCACAGTTTCATGAATAATATTTGGTTTAATGTTATCAAAAGCTATATTTGAAAAATAGTATTCATAAAAATCTTTTTTATTTTTTCTTCAAAAACTTCTTGAAAGAATACTTTCAGGATTCATGTTTTTAAATTTTTTAGAATATAAACCATCAGCTGATCTAAAATCAGGAATTCCTGAAGCTGTTGAAACACCAGCTCCACTAAAGAAAACAATATGTTTAGATTCTTTAATTCAATTTTTAAATTTGTCTATTTTTTCATTAAAATTTTGCACCATATACCTCTATTTAATTTTAGCAAAAATATAATTTATGTTTTTGCCAGCTTGACTTCATTTTGCTTCATAGCCTGTCATAACATTATCTTTAGCATATTTGCTTTTATGAAAATCTGTACCATATGCTATTATGTTTCAATCGTTTTCTTTAAAGCTTTCTAGCGAAAATCCATAAAGTTTATCATTGTCTGATTTGAATTTTAGAATAGTATTTTTATCCATAATAAGTTTATATTTTTCAAAAAATCTTTGTAAGTTAATCTTCTTTTAAAATGTCTAGCCTTAGGTCAAGGATCACTAAAAGTTAATCAAATTGTGCCACAAGTTCCTTTAAAAATGCTTTTTAATTCTTTAGCATCTTCTAAAATAATTTTAAAGTTATTAAGCTTATATTCTTTAGCTCTTCTTACGCTTTTAGCAGCTACAGTAGCAAACTTTTCTAAACCATAAAAATTTATATTTGGATTTAGAGCAGCTAATTGAGTTATCATTTCGCCTTTACCCATGCCAATTTCAATTACATCAGTATTTTTTAAGCTAATAGGATATTGACTTTTAGTAATCAATAAATTAGATTCTTCAAGCTTTTTTGGTGCATCTTTGTCATTTCTTAATCTCATGTTTTCTATTTTACTATATAAAGGAAGTTAAATATTAATTTAATATTTATAAAAAAATAAAATGTTATAATTTGTTATAAGTTGTTAGCGAATGGAGGCAAAAGATAATGGAATTTAAAGACAAAATTTTACGTGCTAGAGCTCTTTTAAACTTAACTCAAGAAGAATTAGCAAAACAACTTGGTGTTTCTTTTAACACCATAAATAGATGGGAAAATGGAAAAGTTAAGCCAACAAAAGTTGCTAAATTGAGATTTGATGAATATTGTAGAAAAAATGGTGTTTTGTTGGAGGTTAGATAATGGAATTTAATGAAGATACAAGAGTAAAATTACCAGCAACGATTCACTTTTTAAAACTTGGTTACAATTATCAAACTGTTAGAAGCCCAGAAATTAATAGAGACAATAACATTTTTGAAGAAAGATTTCATAGAGCAATTGAAAAAATAAATAAGAAAAAATTTGCTAAAGAAGAAATAAATGAATTGATCTCTGATATAAGCAGAAAAATCAAAAATAAGGATTTAGGAAAAGAATTTTATAATCGATTAATTTCGACCGATTACCCTATAAAACTGATAGATTTTGAAAATATTGAGAACAATGATTTTGCAATTGTTAATGAATTAACTTTTAGAAGTAACGCTAATTCTAATTATGATTCATTTAGACCAGATATTACAATTTTAGTTAATGGTATGCCATTAGCATTTTTAGAAGTAAAAGTTAAAAACAACAAAAATGGTATTCAAGCAGAATTTATTAGAATGTGAGAAGATAGATTAAAAGTTGATGAATACAGAAAATTTTTTAATATGTTGCAAATTGTTTCATTTACAAACAATATGGATTATGAAGAATTTAATGATGCTGAGGCTTCACTACTAAAACAAGGCTCTTTCTATACAACACCAAATGGAAAAGACAATACTACTTTTTCAGCTTTTAGAGAAGATGAACCTAACTACCATGCTAACTACTCTTTTGAGGAAATAGACAATGACAAAATAAAAGAAATTTTAATTGATAATAATATTAAAACTTCACATGTTGATAGTGATTGATTCCAAACAAATTTATCAGATACAACTCCTTGCAACCGTTTTATAACTTCAATTTTTGACAAAGAAAGATTTCTATACTTCATTAAATATGGAATGATGTATCTTAGCCAAATAAAAGAAATTAAAAATGAAACTTTAAATAAAGTTGAACAATTGCCAATTAATCAAAAACACATTATGAGATATCCTCAATTTTTCGCTACAAGAGCAATTATCAAAAGATTCAAACGAACTAATAAAAATGGAATTATTTGACATACTCAAGGTTCAGGCAAAACAGCACTAAGTGCTTTTGCTACAAAAGTAATTAAAGATTATTGAAGTAAAAAGAAAATAAATGCAAGATTCTTCTTTATAGTTGATAGATTAGACTTAATGAGACAAGCTTCTGCAGAATTTGCCAATAGAGGTTTCAATGTTGTTAATGTTGATTCAAAAGAAGCATTCGCTAAAGAATTAAATGAGACAATCGATAGAAATAATAATGGTATTGGCACAATTTGTGTTGTAAACATTCATAAATTTATGGATAGTTCAAAAATGCCAGAAGCTAAAAATGATTACAACATAAGAACTCAAAGAATTTTCTTTATTGATGAAGCTCACAGAAGTTATACAATGCATGGTGAATTTTACAAAAACCTTATGACTTGTGATAGAGATGGAATTTACATTGCAATGACTGGTACACCTTTATTAACTAAAAGAGAAAGATCAAATTTAAAATTTGGAGACTACATTCATAAATACTTTTATGATAAATCAATAGCTGATGGTTATACTTTGAGAATTAAAAAAGAACAAATTAAAACTGAAGCTAGAGCTACAATTATTAGAAACTTAAATATTGAAATTGAAAAATTAGAATCAAGAATTATTTATGAATCAGATGATTATGTTAAAAGCGTTAGCCAATATATAGAAAAAGACTTTATTGATTTTAGATTTATTTGCAGAAATGATTTAACAATTGGTGGAATGATTGTCTGTCGTTCAAATGACCAAGCGAAAAAAATTCATGAATGATTTAAAAAGAATTCTAAATTAACTACTGGCCTTGTTTTATCAGACACTGATAATCCATTGCAAGACAACATAAATAAAAATAATCAAAAAACCTTTAAAGAAAGTAATTATCCAGATATTTTAGTTGTTCACTATATGCTTACAACTGGATATGATCTTAAAAGACTTAAGAAGATGTATTTATTAAGAGGACCTAAAGCTCAAAATCTTTTGCAAACTATTTCACGTGTTAATAGACCCTATCGTAGTCCTAATGGCCAAGAATATCAATATGGATATATAGTTGATTTTATTGATATTGAAAAAGAATACAACAATACTATTGATGCTTATCGTAAAGAAATAGAAGAAGAATACGATTTTGAAGAAGAAGACACTTCCCTTTCTGGTTTAATTATTGATAAAACAGATATCAAAAAAAGATATGATGTTTTAAAAAGTGATCTTGAAAAATTTGTTAATAATTACAATTTAGAAACCTTTGTTGACAAAATTAACAATTTTGACAAAGATACTTTACAAAAGATCAAAAAGATTCTTGTTAATATTAAAAACTGTTCAATTGAATTCAAATTATCACAAGCTGATGAATATTCAAAACTCATTAACGATTATGAAATCAATGTGTTTATTAAAGAAGTACAAAAGAAAATTAATTTTTTAAACTTGCGTTCAGATCCTATTGATACAATTCAATTAATAGATAACAAAGAAGTTGTTCAAATCATTTATGATTTTCTTTTAAAAAATATTACTGTTTTAGATTTAGGTAAATTTGATAAAGAAAATAAAATGGAACTTATTAAACAAAAGTTTATGAAAATGCAAAAAGAAATTAAAGAAAACAAAAATACAAGAGATGTTAAATTTATGAATCTTAATGCATTGATTAAGAAATTACTTGATAAATTAGCTGTTGAGGAATTGACTTACGAAGAAGTAGAAGATGAATTAGATTTAATTTACAATTCAACTAAAAAAATAAATGAAGAAAATAAAGAAATATCTAAAGACTTTGGAAATAGTTTGGCTTTTGTCACAACAATGAATATTGTTTTAGCAGAAACTAAATTAGATCGTTCAATTTTAAAGAACTTTTTATTAATGGTTTATGATGAATTAAAAGGCAACATTTCTGACGAAATCCTCATTAAACAAGGCAAAAATGGATTTATTGACTCAACAAAATCTAAAATAACAACTAGATTAATTAAGAAGAATTTATATAAACAAATTAAAGATAATTACGATTATATTTTGAACGAATTATACTTTAATCTGCTTAATTACAAGGAAACACTTTAAGGAGAAAAATGACTAATTTTACAAAACTTGAAAATAATATAAAATCAATAATCGATGATTTGAAGGGATTGTGTTCAACAAATGGTCTTTCAAATACAGCTGGCGAAGAAATAGTTGTTACTTCTGTCTTTTTATATAAATTTCTAAATGATAAATTCATTTCAAATTTAAAAAAATTTGCAAAAGAATTAAATGTTCCTTTTGAAGAAATAATTAAAAATTCAAACTATATGGATGGATTTTATGATGCTTATCCACAAGATGTTGCATTCAAATATGAAGATACAATTGATTATTTAGTTGGATACATTAATGAAGATGATTTTTATCAAAAATTTGACGATACATTAATTAGAATTTCAGATTATAAACAAAATGAAGCTTTTAATGTTGATACTGCAGAAGGAACTAAAAAGCCACTTTTTGTTCGTCTTAGTACTTTTGTTGAACCTTCAAAAAGAAATAACTTCACAAAAAATGTTTTTTCTTACATTACAAGAGATAGATTTGATTTTGGTGAAGCTTTTAATAATAACTATGATTTCTTTTCAACTATTTTTGAATACCTAATTTCAGATTACAACATTGCAAGTGGTAAATATGCTGAATACTTTACACCTCAAACATTATCAAAAGCTATTGGTGAAATATTAGTTAAAATGTCTCCAATTGAAGATAAAATTTATGAAATATATGACCCAAGTGCAGGTTCTGGATCGCTTGTGTTGCATTTAGCAAATGAACTTGGAGAAGGTAAATTTGGAAATAAGGCTCGTGTTTACACTCAAGATATTTCACAAAAATCATCAAGATTTTTAAGAATAAATATGTTACTTAATGGTTTAAAAGAATCACTAGATAACATAATTGAAGGCGATACCTTACTTACCCCAACACACTATAAAAAAGCAGGAGACGCAACAACAGGTCTTAAACAATTTGATTTTATTACATCTAATCCACCATTCAAAACTGACTTCTCTTCTACTAGAGATAACATTGAATTAATGTGACAAAATACAGAAAGATTTTTTGCTGGTATTCCTAAAGTTCCCAAAACTAAAAAAGATTCAATGGCTATTTATCTTTTATTTATTCAACATATTCTTTATTCATTAAAAGAAGGTGGTAAAGCCGCTATAGTTGTACCTACAGGGTTTATTACAGCACAAAGTGGAATTGAAAAAACTATTAGACAATATATTATTGATAGAAAATGACTAAAGGGTGTGATTTCAATGCCATCAAATATTTTTGCTAATACTGGAACTAACGTATCCGTTTTATTTATAGATAAAACAAATACTAATGGTGAAGTGTTATTAATGGACGCTTCAAAAATGGGTCATAAAGAAAAAGTTAAAGATCTTCAAAAAACAGTTTTAACTCATGATGAATTAAACAAAATAGTTAATGATTTTGTTAATCATAAAGTTGAAGATGATTTTACAATTTCAGTTACCTATGATCAAATAAAAGAAAGAAACTATTCATTATCAGCTGGTCAATATTTTGAAGTAAAAATTGAATATGTTGATATTACTCAAGAAGAATTTGACAAAATGATTGATGACTTTAATAAAGAATTAGAACAATTTACAAAAGATACAGAAGAATTCCAAAAAGAGATGTCTAAAACATTGAAGGAGTTGAAGCTAAAATAAATTAATAAGGATAAATATGGAATATAAATTAAAAGAATTAGGTATATTTGAAACTGGAAGCACATTAATCAAAAAAATTGGAAATTTTCCTGCTTTTGGAGGCAATGGCATTATTACATATGTGAATAAATGAAATGTTGATGAAGATGCAATAATTATTGGTAGAGTTGGAGCGAATTGTGGTTGTGTAAATATCACAAACAAAAAAAGTTTTGTTACAGATAATGCTTTAATATTTAAACCTAAAGAAAAAAATATGGCAAGATTTTATTTTTATTTTCTTTTACATTTAAACTTAAATAAATTTCATATAGGTTCTTCACAGCCTTTATTAACTCAAGGAATTTTAGGAAACATAAAAATAAATATTCCTAGTTTAAATAAGTGCCAAAAAATATCAAAAATATTAGATAACATAGATAATCAAATTGAGCGAAATAATTCTATGGTTCAAAAGTTACAAGTTATGGGACAGGCTATTTTTAATAGGTGATTTTTACAATTTGAGCATTTTAAAAAAGATAATAAATTTAAATACAATGAAGATTTAAATTTAAAAATTCCTGAAAATTGAGAAGTTAAAAAAATTGCTGAAATTTGTAAAATTTTTCTTGGAGGAACCCCTAGCACAAAAAATAGAGAATATTGAAATGGCGAGATAAATTGACTAAATTCTGGTGAGGTAGCCAATTTTCCAATAATAGATAGCGAAAAAACAATAAATGAAAAAGGTTTAAAAAATTCAAACACTAAATTATTGAAAAAAGGTACTGTAGTTATTTCTATAACAGGAAATATAAGGGTCAGTTATCTTGCAATTGATTCATGTATAAATCAATCTATAGTAGGAATAGAAGAAAATGAGTTATTGAAAATAGGATATTTATATCCTTTTTTAAAGAATAAAATTGAGTTTTTAATAAGATCTTCTACTGGGAACTGTCAAAAACATATAAATAAAAATTTTATAGAAAATTTAAAAATTGTTTTACCACCAAAAAATGTTTTGGACATATTTAATAATTTAACTCAAAATATATATGCTAAAATAAGTCAAATCAGTTTAATGACTAAGAAATTAATAAAATTCAAAAATAAGCTATTACCTTTATTGATTAATCAACAAATTATTTAAAAAAGTCCTATCAATAGATAGGGCTAATTTATTTCTATTTGGCCATTTATTAATAAAGGTATCAACTTTTCTTTAAGCTTTATCAAACTTAAAGTATTTTTTTCTAATTTCATGCATTTAGTTATTAATTCGTTTAATAAATTGTTTTTTTCGTTTGTTAATATTGGAACATTCACAATATTGAGATGATTTTTGTTAATAATTTTTTGTATAGCTCCAATTGATTGTCTTTTTAAAAATGAACTATATTTATTTAGAATAAAATAAATTAGTCCAAATTTTTTTTCATTATAAAATCCAACCATAGCTCTCCCAATTGCAATATTTTTATCACTTATTCCAATTTTACCAACAGTTCCCGCAATAGACATACAAATTGTCCTATTATCTATTATTTTAATTGGTTTATCAGTAAAAGCTTTAGGAGAAATTAATATTTTATTATTCTCTAAATCACCTGCTCCTGATGCATAAAGCATTAAATTATTTGATACGTTTTCGCTTAAAATGTTGTTTCCTTTTGGACACTGACCCCAAATTATTTTGCACAAACTTTCTATATTTTCATAATCTTTCGCATTATTCAAAAACATATCAAAAATAGCCTGTCCCATAACTTGTAATCTTTTGACTATATGCAAATTCTTTTCAATCTGCTTGTCTAAATTTTCTAATATTTTAGAAATCTTTTTTTGAGTTTTTAGTGGAGGCAAATCAACAATTTTTTCTTCCATTGTTTCTTTTGTAATATGAATCATTATTGATCCATTAGTTGATGAAAGCCAATAAGGTGTCATATACATTAAAAGATAATAAAGAAACATTTTATCGACGCGCTTTTCATCTCATTTTATTTTTCATATATGATAATGAAATATTGTTTTTTCAGATTTCCAAATTTCAGGGCCGAAATTACAAGCTCATTTATATAACAAATCGCCATTTTCACAATATTTATCTTCATTCAATTCCATATCTGAATAAAATCATTCATTTTTACCAGAAAAATTTCCTACTCGCACTATTCTATATTTTCCCTTGTCTTGTAATTCAGGTTGAGAATAGGCTCTTCCATTAATGAAAGTTACAATATCTTTCAATTTAACAAGCATAATTTGCCCCTCACTTTTTTGAGAAAATTAATGCTTGCTCAAAAGACTGTAACTTTTGAAACTAGCTATTTTTTAGTGCTTTTTTAGCTCTAACTAATGTTGATTTACTAATACCAGTTAAAAGTGAAACTTGGCGATAGGAAAAGGAATCTAAAAGCAAAATTGCTTCACTTAAAAGATCTTCAGTATAAACTTTTGGTCTACCTTCTTTATAATCTGTTCTTAGTCTTGCTATCTCTTTACCATTTTGTGTTCTTTCTACTATCATGTTTCTTTCTAATTCAGCAACTGCTAACATAGTTGTTAAGAAAAAACGACCCATTGGAGTGTCCTCTAATAAGACTATATTTAAAATATGTATTGCTACTTTCTTTTTAAATAAAGCTTCAACTACTTTAATACCTTCAACTGTATTTCTAGCAAATCTATCTAATTTAGAAACTACTAGAATATCATCTTTTTCTAATTTAGTTAATAGATCATTAAATAGTGGTCTATTTAATTTTGTTCCTGTAAATTGTTCATTAATTATTATTGCGTTTGGATATTTATTCAATATTTCATTTTGTTGTATTTCAAAACCATATCCATCAATTTGTTTTTTTGTGCTAACTCTTGTATAACCATAAATCATTTTTTTACCTCGCTTTGCAATTAACACATAATTAAATAGTTAATATTAATTCTTAATTTTTGAGCTCAAAAAGCAATAAATAATATTTTAAATAATTTTTGGCACTCTCACCCTTAAAGTGCTAAAATTATGATATAATTGATTTTCTATAGGAGGTGAAATTATGGATTTTAATTTAGATGACTTATTTGGTTCTAATGACAATAAAAGTAAAAAATCAAGTGATCCATTAAAAATTTATGGAAGAAACTTAACTGATTTAGCTGCTCGGAATGAATTAGATCCTGTTATTAATCGTGATGATGAAATCAGAAGAATGATTCGCATTTTGAGTAGGAAAACAAAAAACAACCCAGTATTAGTCGGCGAACCAGGTGTTGGTAAAACAGCTATTGTTGAAGGATTGGCTAGAAAAATAGTTGAGGGTCAAGTACCAGAAAACTTAAAAGGTAAAGATATTTTTGAACTTGACTTAGCTGCTTTAATAGCTGGCGCTTCATATCAAGGACAATTTGAAAAGAGATTAAAAGACGTACTCAAAAGAATTGAAGAATCTAATGGTGACATTATAGTTTTCATTGATGAAATTCACATGCTTATTGGCACAGGTCGAAATGCTGAAGGTGGTATGGATGCTGCTAACATCATTAAACCTTTAATGGCCAGAGGTAAAATGCATTTAATTGGTGCTACAACTTTTGATGAATATAGAAAATATATAGAAAAAGATGCGGCTCTTGAAAGAAGAATGCAAAGAGTGGATGTATTAGAGCCTTCAATTGAAGATACTATTACAATTCTTCGTGGTATCAAAGACCGTTTTGAAAATTACCACAATGTAAAAATTCAAGATGATGCTTTAGTAGCTGCTGCTCGTCTTTCTTCAAGATATATTTCTGATAGATTTTTACCAGATAAAGCTATTGACTTAGTTGATGAAGCTGCTGCAACTATTAAAACAGAAATAAACTTTCAACCTGAAGAATTAGAAAAAGCTAAACAAAAAATGGCTAGATTGAAAATGGAAAAAATAGCCATTTCTAGTGAAGACAAGAAAAAACATGCAGTTAGACTTAAAGAAATTGATGCCGAAATAAAAGATATTGAAAAAACAATAAAACAATTAGAAGATAAATGAAATTTTGAAAAACAAAGACTTGAAGAATTGTCTAATTTAAAAAGTAAATTAGATGACGCAAGACATAGATTAAATATTTACCAAAAAGAAACAGATTATGAAAAAGCTTCAAAAATTTTGTATGATGAAATTCCAAGCATTAAGAAACAAATCGAAGAAACAGAAAAAGAAATAGCAGAATCAGGTAACACAATTGTTAAAGATAGTGTTACAAGTGAAGAAATTGCTAACATTGTTTCGAAATGAACTAAAATTCCAATTTCAAAATTACTTGAAAGTGATAAAGAAAAATTGTTAAATCTTGAAAACGATTTAAAGAAAAGAATAAAAGGGCAAGATGCTGCCATTAGATTAGTTGCTCAAGCTGTATTAAGAGCTAAAGCAAATATTAATGATCCTAATAGGCCTTTAGCAAGTTTCTTATTTACAGGACCTACTGGTGTTGGTAAAACAGAATTAGCAAGAGCTCTTGCATATTCTCTTTTTGATAGTGAAAAACAAATGATTCGTTTAGACATGTCTGAATACATGGAAAAACATAGTGTTTCTAAAATCATTGGTGCTCCTCCAGGATATGTTGGATATGACAGCGGTGGATCATTAACTGAAAAAATTAGAAAGAATCCTTACACAATTCTTTTATTCGATGAAATCGAAAAAGCTGACCGCGATGTTCTTAACATTCTTTTACAAATGATGGATAATGGGGCTATTACAGACTCAAAAGGCCGTGTAATTAACTGTCGTAATTTAATTATAATTATGACAAGTAATTTAGGCTCACAAGAAATACTTAAACATTTAGATCAAATGCCAAGTATTAAAGCTGAATTGCTTAAATTCTTGAGTCCTGAATTTGTAAACAGAATTGATGAAATTGTCAAATTTAATCCATTAACTAAAGATGTAATTAATGAAATTGTTGAACTTGAACTTCAAAAATTAGCTACTAGAATGAAAGATGTTAAAGATGTAAAAATATCATTCACAAGCAAAACTGTTGACTTTGTAGGTTCTTCTTCATATGATGAAAACTTTGGGGCAAGGCCTATTAAAAGATTTATCCAAAATAAAATTGAAAGCTTATTAGCTTACAAGATTATTGATGGCACCATAAGAAGCGGAAAAGCCTATAAAATAGACGTTTTCGCCGGAAACTTTGTTGTTAAAGAAGCCTAAATTTAAATAAGTATTGTTACAAAACAGTACTTATTTTTTGATCAAAAAAAGACCTTTAAAAGGTCTTAATTTTTAAAATAATTTCATTTGGTCAGTATCGTCAAGACCTTTAAAAATGTTAAGTTTATCCATTTTTTCAATTAATGTTTTATTAACTGAAGTACGGCGAATAACATCTTCTTTTGAAGTAAAATCTTTAGCATCTCTAGCTTCTACTAAAGATACTGCAGCTGCATCACCTAATCCATCAATTGATTTAAAAGGAGGAATTAAACAACCGTGTTTATTGTCAATAATTCATTCATGTGCTGCCGATTTTTTCAAGTCAATATTAGCAATATAGAAACCACGAGCATAAAGTTCTTGAGCTATTTCAAATACTGGAATTAAGTCATCATCTTTAACTTTTCTTTCATTCTTTGGAATGTTATTCAATTGATTCAATCTGTCTGTAACTTTATGTCCACCTTTAATATCAATCATACTTTCAATTTCAACCGCTTTAGCATGGGTTGCAAAATAACAAGCATAGTGAGCTAAAGGATAATAAAGTTTAAATCAAGCAATTCATCAAGCCATTAAAACATAAGCGGTAGCATGGGCTTTGGGGAACATGTACTTGATTTTCTTCATACTTTCAATCATTCAGTCAGCCACTTTATATTCTTTTAATCTCTTAACTTCATCATCAGTTAAACCTTTACCTTTACGAACTTTTTCCATAATATTGAAAGCAAATAAAGGTTCAACTTTTTTCTTTAAAAGAAATTGCATAATGTCGTCACGACAACAAATAACATCAGTTATTTCAAAATTCTTTTTGCTTACTAATTCTTGAGCATTGTTCAACCAAACATTTTCACCATGAGAAAGTCCAGCTAAAGAAATAAGGTTAGCAAATGATTTTGGTTTAGCTTCTTTTAACATTTGACGAACAAAACTTGTTCCGAATTCAGGCAAACCTAATGCCCCTGTTGTTTCATTATTAATTTGTTCAGGTTTAATATTCATAGCTTTAGTACTTGCAAATAAACTTAAAACTTTAGGATCATTTTTTGGAATGTCATCAATATTAATATTGGTATATTTTTTAAGCAATCTAATAATTGTTGGATTATCATGACCAAGTAAGTCAAGTTTTAAAATGTTGTCATGAATTGAATGGAAGTCGAAGTGACTTGTTAATCAAGAAGAATCAGGTTCATTGGCTGGATAGTTTATTGGGGTAAAATCATAGACATCAAATTCTTTTGGAATAATAAGAATACCACCTGGGTGTTGGCCTGTAGTTCTTTTCAATTTTTCTATTTTATGGGCTAAAAAGTCAATAAATGGTTCTGAATAATTACGCATTGCTTCTTCTGTAAATGCTTTAACATGTCCATATGAAGTTTTAAGAGCAACTGTTGATATGGTACCAGCTTTTAAAGTGTGAGTATTACCAAAAATACGTTTAACTTCATTGTGAATATCACCTTGAATATCGCCTGAGAAGTTAAGGTCAATATCAGGAACTTTATCAGCTTTAAAACCTAAGAAGGTTTCAAAAGGAATTCCTTGTCCATCAGCTTCTAATAAAATATTACATTTAGGGCAATTTTTGTTAGGTAAGTCATATCCACTTGTTATTTTTTCATCTTTTGACAATTCAAAATATTTACATTTGTTGCAAAGATAATGAGGAGGTAAGGGATTAACTTCAGTAATACCTGCAAGGGTTGCAACAAATGAGCTACCTACAGATCCCCGACTACCAACAACAAAACCATTGTCAGTTGATTTTTGAATTAAGATATGACTAATTCAATAAATAACACTAAAACCATAATCAATAATAGGATTTAATTCGGCTTCAATTCTTTCTTCAACTATTTTTGGTAATTTTTCACCATATTTTTTGTATGCTGTTTCATAAACTAAATCATGTAGTTTTTTACCGCTGTCTTCAAACTTAGGTGTATAAAGTTTATCTTTGATAACTTGAATTTTGTCGCACATGTCTGCTATTTTATTTGTGTTGTTTATAACTATGTCTTCAATTAATTGTTTGTCTTTTAAAAAGGCAAATTGTTCAAGCATTTCTTCAGTGGTTAAATAGTCTTGATTTGGAATTGGAATATTTCCGTTTTTAGCTTGTTCATAGTTGAATAAGTAATGACGAGAGTTTTTAATTCCTTTAGCATAAACTAAAACCTCATAAGCTACTTTGTCATATAAATTTAGATACTTAACATCAGCTGTAGCAACCACTATTTTTTTAGCTTTTATTGCTTCTTTAATAATTTCTTTTAAAGAAATTTCTAATTGTTCTTGAGTAATAAATTCATTATCAATTCAATGGCTAAACACTTGAGGTGCTGGCACTTCAATATAGTCATAAAGTTTTAATTCTTTAATGAATTCTTCATGTGAAGAATAAAAATAAACATCAAGCAATCTACTTTTTAGTGTTCCTGAACCTAATAAAAGATTTTCACTATGAGCTGCTAAATCTTCCATAAATGTTTTAGGTCCATTGTATAAGTTTTTAGTTAAAGCTGTTGTTAAATAACCATATTGTTCTTTTAAACCTTCATAGTTTTTAGCTATTGTTGAAACTTCATATGAAAAGGTTTTATTATAAAGATCATCTTTAATATATGAATTAAGTTGATTAAAGGTTAAAATACCTTTTTTACCTAATTCGTCCATTAAAGCCACTCAAACTTCAGCTAAAACTTTCGCATCATAATCACCACGGTGAGCAACTGTAGGATCATATTCAACACCAACACGAGTAGCAACATCTTCAAGTTTATGTTTTTTGTTTTGAGGGAAAACAATTCTTGAAACAATAAGTGTGTCAATTACTGTTACATTTGGAAATTCTATTTTAGCTTGTCTAAATTTTTCTTTTAAGAAGTTGAAGTCAAATCTAGCATTGTGTGCTAAAGCAATTTTGCCATTTAAGTCATTATAAATTTTTTGCAAAGCTTCTTTTAATTCCAAACCACTTTTTTCAATCATTGCATTAGTAATACCTGTCAAGTTTTCTGTAAAAGGTTTAATTGGTTCTTGACATTTAATGAAAAATTGTGTTTTTCTCCCATACTTCATGTTTGAGTGAATTGGCAATGAACCATATTCAATAATTACGGCAAAACGAGGACAAAGTCCAGTGGTTTCAATATCGAATGAAACATATTCTTGACCTTTAATTTCGCCTTCAGGTATGTTTCCTAAAATAGATTTATTACTTTTGTTTATAACTGAAAACGCAGTTCCAAAAAGAGGTTTAACTTTTGATTTTTTAGCTGCATTATAAAATTCAGGATAACCTTGAGCACCATCAACGTCCATAATTGCAACAGCAGAATGATTCAATTTTTCAGCAAAAGCCACAATGTCTTTAGGAAAAATTAATCCATCCATTGTGTTCATTTTTGAGCTAACATGTAACTCAACTCTTTTTTTAGCTATGTCTTTATTTTCTTTCTTTTCTGTTAGTAAACTTTTTGCTTTTTCATAGAAATCTAAGAAGACAAATTTGTTTCTAGCACCAAAAGTACTTTCAGAAACTGTTCCATAAATTGTTACTCATTTGTTGTCTTCAAGCTCATTTATTTGTTCAGGTTCTAATAATGTTGTTCTTATTGTTTTTACTTCTACAGCATCTTTATAGTTGGTTAGAAAAAATGTGTAAATGTGTTTACCATTTTTACTTAAAGCATAGTCTTGAGAATAAATCATTCCAGTAAATGAAACAGCAGTTTTATCATCTAACGAAGATATGTCTTGGAGCTCAACTTTTTCATAGCTTTTTCTTCTTCATCTATTCGAATTATTTGGAGTTGTAACTCTTTCATTTTCTTTGGTAAGTTTTTCTTGGTTTTCTTGTCATTTTTTCATAAATTCTAGAGTTCTTTTGTTTTCTTCCTCAATAACACCGTTGTCAACTGAAACCATGGTTTTTTGTTCAAAATTAATTTTTAAATCTTTTCAACCGTATTTATTTAAAGTTTTTTGAAGGTTTTTAAAAACGGGGTTAAACTCTTCATATTCTTTAGCTGAAATAGTTGTAATTGTTGCTACAAATTGATCATTAAATTTAAGTTTTTTAACAATTGGCAAATTTCTCAATTTGATGAATTTGGCTTTTTCATAGGTTAATTCGTTAATAAAATCTATTAAATTTTCTTTTTTATATTGTGAAATATCAAAAGTAATTTTATCTTTGATGTTATAAACTTTATTATTTTTTAAACTAGAAAGAAACGAAAAAAATTCCTTTGCACTGATTGAATTTGTAAATTTAAATGACACATTTAATTGGTCTTTAACACTGTCATATTCGACATCGTCTAATTCAACTTTATCCAATCCAGGTGTCACTTTTACATTAATATCTTTACAAAATGTAATGAAATTTTCATTTTCTATTTTTCTATTTTCTTTAGGCATATTATTACTATTATACATATATTTTTTTATAAAAATTTAATTATGTAAATTAATTTATTTTAATAATTTTTTAGCCTATTTTTAAGCTTTTTTATGTTAAAAATTAACAAGAAAAATACCACTAATTTTGTGGTATTAAACCAGGACAAAAGAATTAGACTTAAATCCCAAAAAGATTTAAGTCTTTTTGTACTATGAAGTTTTATGGGAAAACATTTAAATACTGAAAAATGGATAGAAATTTTTATATTTTTTAAAGAATATTTAGACTATAAAATTACAAAGATGGAATTTAAAATGAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGATGGCTAATGCCCTCACTTTTATTTGTCCTATCGGAAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGGATTAGCCTTTAAAGCTAATCATTTTTATTATTCTGGATAATTTGATATTTAATAAATGTGTCTTTAAACATATTAATATCAAAATATGTTAATTTATCAGGCTTAGTATTTTCAAAATCGAAGATTGAATTTGCATATACTAATTTTCTTTGCTTTTTAGGAGATAAGTTTTCAAAATTTTCTGGATATTTAATATAAAAATCGACTGATCTGAAAAAGAATGAAACTAACGACTTTTCATTTGGAAGGGAGTTATAGTGATATTTTTGGCCTAATGAAAAATCAAATTGTGTTTTGTTAGTTTCTGAATTAATCTTCTTTTCATAAAGATTTTCGTCATTTAAATTATTGTTGAATTTCAATCATTTGGTTGTTTTTACAAATATGTTTATCTGTATAAGGATAAAACAAATTACTAAAAAAGTGCTACCGCCGAATATAATTCAATAGTATGCAAGCGAATCATAGTATTTGATATTAGGCTTTCATAATAATCCTACTAGCAAAGCAATAAGTATTTCTATTAACAGTCAGCCCAATGAACCTAACATATAGAATAATTGATATTTTTGTTGGTTTTTGATAACAAAATCTTGAAGTTTTATTTCATTACTTATGCAATTAGGTCTTACTGTTAGACAAAAATATAAGATTTTTTTAAAGGTATTTTGTTTGACTAAAATTGAATCTATGAACAAAGGCATAAGTGTAATAAAACATATAATAATCACTAATCAAATCATTTTTAACTTTCTAAACAATGAATAATATATTATTCAATAATTTTATAAGCTTTTGCTTCATTTTTGAACATATTAATATTTAGCGGCTCAAATTTATTATCAAATGCTGTATCATCGTAATTGAGAACCATAGTCATATAAATAAAAAGTTTTTGCTTTTCGAATGAGTATGACTTGAATTTTTGAGTATTAAAAGTTGAAAAATAAAGTGCATTAAAACCTTGTCTTTTAAAATAACATTTATTTTTAGATGGATACAATTGTTCAACATCTGTGTTTATGTCAATTGATTTTTCGATTAAAGAACTATCTGGAAGCATATTATTAAACTTTCTTCACTTTTTTTGTTTTATATATCATAGTAAGCATACAACAATATATCACAAGGGCAATGGTACATAAAATATTATGCCAAGTGTTACAAACATTGTAAAAAATGATTCCTTTTCATATACTTGTAGAAAAACCATTAAAACTAAGCTTTCTAGCATTAATGCGATTGCAAGTAAAGCATAAATTAATGGAATTTTAGACATCAGACAATAATGATTTACTAATGAATTTTGAACTCTTAATTTTCTTTTCATGCAATATTTATTTACACTACCAAAAGCATATATATATCATCTAATTTTAGGAAACAAAATAATGAATGGCGTAAGATTAATAAAAGCAATTCAAAAACTAAAAAAGATAATTGAAAAAATTAAAAAATTACTCATATGTGATTATCCTAAATTATCATATAAAGAATTATTAGCTTTTTTACGCAAATATGTTCCTTTTTTAGGGTCATTAACTCTTTGTAAACTTCACAAACTTAATTCAAAATCAGAAAGCTCTAGCATTTCATTAATAGTTTTTATTTCGTTGGTTTCTAAATTTTTAAAAATAAGGTTTTTTCCACCAGTACCTCCCATATAATAAGGACCAGTTTGTTCGCTTTCATGTATAACAACTCATCCTATTTTTTTAATATCTTTTATTTTCTTTGAAAAATCACTAATTAAATCTGATGCCTTTTTAGCACATTCATTAAAATAATCTAATTCTTTTGAAGCGGTGTAAACATCATAAAATGATCCAAGAGTTTGTAATGCTGAACCAACAACACCAGTAGCCATAGTTGCTATTGATGGCGCAGCTTGTGATAATTTAAGCATTTCATATACTCTAGCAACACGATTTAATGCCATAGGAATACTTGAAACCATACCTCATATTGGTATGCTATAATTTGACGATTGAAATAGCTCAATAAATTTTTGAGAATTTTTTAGAGTCTCGTTTAAATAATATATTCTATCAGATAATGTAATTGCGGCAATTGATAGCAAAGATGCTGACGCTGATGCTACTGATGCAGCAATTGCACATGGTGGACAGAAAAAGCTAGCTATTCATAATCCAGTTGCTGATGCTGCTGATATGGCAGATAATGAAACACAAGCGATTTTTCATTTTTGCAAGAACGCAATATGATCTATTGTTTTTTTAATTGAATCATTAAGGTTAATTAAATGATTATTCAATAACTCATAATCTTTTAAAATAAATTTAGATGAAATTGTACCGTCATCATTTATTTTTCAATCTTTATTTTCAAAAGACGAATTGACTCTTTTTATCATTGAATTGATTTCTATTTTTGATTTTTTTTCGCTTTTTTTAAAAGTTAAAGATTGTTCTTTTATTTTTTTAATTAATTCAGTTTTAAAAATTTCTCTTTTGTCTCTTAGTTTATTTAATAATTCATCTTTATTTTTTATACTAGATTGGCTAATTTTAGATATAAACAAATCATAAATTTTATCTAATTTTATTTCATCAATGGAATTTATAACTTTTTTAGTTGAATTTTTGTTATTGATAATATCATTTGAGATATAGTTAAAAATTTCAATTTCATCGTTTGTAAATCGAGAAAATAATTCCAAAATTTTTTGTTGCTCTGAATTTAAATTTTCATTTGATGCAACAATGACTGATGGTGTTATGTTAGTTACATTAATTGTATTTAGTAAAGTAGATAATTTCATAAATTATATTAGCAAAATGCCACTCTCCATTTTATTATAAGGTTTTTAGATGCTACGACATTTCATATTTATAAATAATAAGACAACGATCCAAAACAAACATTAGATATTTATATAACTGCATTGGGAAATTGTTATTAATATTTTTAATAAATATTACTATAAAACAGCACTAAAAGTATTATAGAATATATATAGTCCTTAACTTTAACTTCCTAAATTAATAACTGTATATATTCTTAATTAGATTATAACATGAAAAATTAAACTAGGTGTAATAATTGATTAGTTTCTGTTTTAAATACTCATTTATTAGATTGTTTAAATGCTAATACAATTTTATAATTGTGATTATTCCCATATGCTTTAAATTAATAAAATGATTGCTGGGTTTAACTTGTATTAAATAAGTAATATTATCGTAGCAATTAAATCAATGCCTATAATCGCGTCTATTAATATGTCTGAGACTTTTCTTTATAAGTCAATAAATCTAATAATTCCTTTTCTCTGTTGTAATTAATAAAACCTTGATAAAAAATAATTTGATACATATTTAAAAAATTTGTTTTGGTGTTGGCTTTATGCTTACTTAGATAATGACCTAATTTAGCACAAAAAACGCCACTATATGCCTTTTGGAACTCTGTAAAGCTTTTATAATTTTTAGTCCACAAAATCTTTTTAATATTTATATTTAAATCTTTATAGGTAGGAACTTTTAACATTGAAAATAATTGTAAAATAAAAAACAATATTAACTGTTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAATTTTTTTTATAAAAAAGTCACTCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCAATGTTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTCATTGAATTTTGAACTGAAAAGATTTATTAAGAAAATTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTGTAGGCAAGAGTAAAGGCACACATTGTCATTTCCTTAGTTTTACCGAAAGAGTCTCTAGATATGGATTTTTGGTAAAAATCCATGAAATTTAAATTTGTGTTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATACGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAAACTAATTTTGATGATATTATTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCAATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGATACCACTAATTTTGTGGTATTTTAAAATTATATTAAGGTTTTATAACTGCACGGGGAGTATAGTCCAAATAATAAATTAAAAATTCGTCAATAGCATTTTTTCTATTTTTATAATATGTCGATTTTGAAAAATAATCCTGGTATCATAACTTGTTTTCAGATTGTTTCATAAACTCATTTTTAATGATCAGTTGACTTACACCGCTTAAGCAATTGTATACTCTTTTGAATTTGTCTGAAAAGTAATTATTAGATCTTGAATTTTCATTCTTCTTGTAATATTTTTCTATAAAATCTTCTTGACGATTTTTCAAGAATATGGCTTTTTCCATATTAATTATTGTTTCTACACTACGATACTTTTGTAAATTACTCATTTGGTATTGTGAAGCAAATTGCGAAATAGTACTTGTCATATTACCTTTCTTTTTTAAATCAGGTAAATAAAAGTATATTGCTAGTAAGCTCTTGCAAAGATTACATATCTATTGGTTTCTTTACAACATTTTTCAAAAATACATTTGTGTTTTTGATTTGGTTTATCAAACTTTTTAGGTAAGCAACGAGTTGAAGCGCCAGTTTCATTTTTGATTATTTCTTCAGCCACATCTAAACAACAAAATGGGGCTATAACAAATTTATGATTTTTAATTTGTTTTTTGAAATCTTCGTAGTTATCTACAAAGACTGTATTTTCATTTAGTCTTTTTTCAGCTTGTAAGTACAAGTTATCGTGGATTTGATTTAAAAGAGTTTCAACTTGATCCTTAACTTCTGATTGTTTTATATTAATCTTTTCAAGGGTGTCTCTTCTAACAATTGTCACACTTTTATTTTCAATGTCTTTAGGTCCAACTTCAATTCTAAGAGGAACACCTTGAATTTCAGAATTTGAGGCTTTAAATCCTGGACCTTTGTCACTTGAATCAAGTCTTACTCTAAATTTTCTACTTAGGTCTTTGTAAAGATTATCACAGTATGTATGAACTTCTTTATTTTTATTTGCAAAAAGCTCTAAAACATCTATTTGAATTGGAGCTACTCGAGGTGGAATAATAATTCCTCTATTGTCTCCATGAGTCATTATAATAGCTCCAAGCAGACGGGTTGAAACACCCCATGAAGTTTGGTAAGCATAATCTAATTCATTGTTTTCAGTTTTGAATTGAATTTCAAATTGTTTTGAAAAGTTTTGAGCTAAATAATGGCTTGTTCCTGCTTGTAAAGCACGACCATCCTTCATCATTGCTTCAACTGTATAAGTTGAACATGCGCCAGCAAATTTTTCTTTTGGCGTTTTCTTACCAATAATTGTTGGTATTGCTAAAAAGTTTTTTAAAAATTTTGAATAAGTTGAAATCATTCCTTTTGTAAATTTTCTAGCTTCAAGCGGATTACTATGACATGTATGTCCTTCTTGTCATAAGAATTCTCTACTTCTTAAAAATGGGTTGGTTGTTTTTTCTCATCTAACAACATTAGCTCATTGGTTATAAATCATAGGTAAGTCTTTGTAAGACTCAATTGAGTTTTTAAATAAATCAGCAAATAAAACTTCTGATGTTGGTCTAATAAATACTTTTTCATCTAAATCCTTGTCGCCAACTTTAGTAATTGTTGCTAATTCTGGATTAAATCCTGCTATGTGATCTTTTTCTTTGTTAAACAATTTTTCAGGTATTAAAAGTGGCAAATATACATTTTGCACACCTTTTTCTCTAAAACATTCATTTAAATTTTTTTGAATTTGTTCTCAAATTCCATATGAATTGGGTTTAAATACAATTGTTCCTTTTACAGGCCCATAAGCAATTAAATTCCCTTGTTTAACAACATCTGTATATCACTTAGCAAAATCTTGTTCTAATGGAGTAATTTTTTCTAGTTCTTTCATAATTTTAATTATATTAAAAAGTTAATTTTTGGCAAGTAAAAAGGTTGAAAAACTAACTTTTTTAAATTTTTGGTGTTTTTTAAATTAAAAAACTTGCTTATTAATTTAGCAAGCTTCTTATATATTATTTTTTATTTCTTTTCTTTGTGATTTTGGTGTTTTCTACATTTTGAACAATATTTGCTTAATTCAACTTTTTCTGGTGTATTTTTCTTGTTCTTTTTACTAATGTAGTTTTCCATTTTACATGATTCGCAAACTAATGTAAAACCGTCTCTTGGCATATTAATCCTCCTAAAAGTTCAAAATTCTTTTTATATTATATAAAGAAATTAGTTTTTTGAAAATATTTTATTTCATAAATTAATTTACATGTATGTAATCTAAACCTAAACTTTGACGATATGCAGCAATTTTTTTAATACTTTGATCAAAATCTCTTGCAGTATTTCCAGTATTTTTTATTTGATCTCAAGTTAATAAATAATAAGTAAATCTATGTGGCATCATTTTTTTACCTTCAAAATAATTTTTCATTGAAGGGCCCATTGTTGGATCATTTCAAGTTGGATCTAAATATAATTCTCGCCCTCCAAGTCTTACCATATTTCAAGCATGACCTTCTTGCCCACTTTAATTAGCAGCTTGACCGGTTACATATCACACAGGAATATTTATTAATGTTAGAAATCTTGAAAATGTTCTTGCATATCCTTCGCAAACTATTCTTCTTTTTGGTTTAGTAAAATATGAATATGGACTACGACATTGACTTCGTATTGGTTCATTATTTGATAAAGCTAACTCTCCATCAGTCAAATATTCATTATTGTCAATCACTCAATTATGTATTGCTCTTATTTTTTGTTCATCAGTCATTTTTTTAGAAATTATTTTATAAATTACTTCTGTCATCAATGAATTTAGTTTAACTTCTTCAGAAGCACTATCTGTTCTATATCTAAATTCTGATTCTTTTATATTTATTTTTTGACGCGTGTAAGTAATACGTTCAAGGATTTTGTCATTAAAATAATTGCTACCACCAGTTATGTCATATCAAGTTGAATTTAAATCTCAGAAATAATCTCCTAAATTATGATTAGTTACGGCAATATCAGAAGTTATTTTTCCTTTAAATAGCAAGTCTTTTCTAATTTTTAAAACTTGTTCTTTTGTGAGAGTATCAACAGCTTTAGCACTTTTTAGAAAGTCCAAATTGTTTAAATCAACTTCATATGCATCTTTAATGTCAAATGATAAGTCTAAGTCTTTTTTATAGTACTTTACAGATTTTTCTATATTGCTTAAAATGTCGTTAAAAAATTTATAATTAAGATTAAGAAGACTTCCTTGAGATATATAATTCTCTAAAATATATTTTATTGTAGGTTCAGGATTCCCATTTATTGTAGCAGTTAATCCTGTATTTGATTGTACAAAACCTATATTAACGAAATTTTTGTAAGCATTTAAATAACTTTCAAATCTTCCCTCATGTAATCCAAAAGCATTAAAAATTTTTTCATTTAATTCTAATAATTCTTTTTTAAGTTTTAAAGCTTTCTTTACTAACAAGTCAACTTTGGAACTTGTTGTATCTTTTACAGTTTCTACATCACTAACAAAATATTTTACCTTTGATAAATATAAAGCTTTTTCAACTTCATTTGCTTCTTTTTCTTCTTTTGTATTTTCTTTTAATCCATATTTATAGATGCTAGATGGTTTATTATCTCCAGGTTTTGGGGCTGGGCCTGGACCTGGTTCTGGTGTTACAGGTGTAACTGTCGTACTTGGTGTTGAAGGTATATTGCCAGTAACATCATCTCAAAAACATGATGTTGTTATAGTTGGTAGACTAACAATAGCAGTTAGAGTACCTAGTCAATTAAATATTTTATTTTTTTTCATATTTATCTCTTTCTACAATTAAATAATAACAAAATTTTAATTAAATATCTTAAAAGTGAACAATAAAAATGCAACCATAAGGCTACAAATTTTATTAAACATATTCATTAGCTTTAAAATAAGGAAGACCTAAGTTAGTTCTTAATTCATATAAATCATGATAACTATCATATAGTTCTCTGGTTATTTCTATCTTTTTATTATTTTTATTAATATATGTTTCATATTTAATATCTTTTCATTCTTTTAATAAATAGTTATTATTAATTGGACTAATTTCTTTTGAATTTACATTGTATTTTTCTTTATATTCATTATAAGAAGGATCATCTCAAGTACAATCTACAAAATATTCTTTACCATTTAATAAAACTAGGTTTCAAGCATGGCCCTCATCATCTTTTAGTTTGCCATCAACATAAGTTTTAACTGGTCCAGTTATATATCAAGATGGAATATCTAAAAGAGTCATAAATCTTTGAAATGTTCTAGCATAAGCGTCACAAACTATTCTTCAATTTCCAATATAAGCATATGGACTACGACAAGTTTGAGCTCATTGTTTGTCACAATATACCATCTCATCATCTCTTAAGTAGTCATTATTTAAAATGATTCAATTGTGAATAGCTCTAATTTTTTGTTCATCATTCATTTGTTTTGAAATAATAGAATAAATAATTTTTTCAAAATATTTATTGATATCAATTTCTTCTTGTGCATTTTCAGTTCTATAAGCTAAACGATTTATTTTAATGTTTATTACATTATTTTCTGGTTGATAAAATGTAACAGATGTTGAATTTTTATAGTGGTTTCCTTTAAAACAAAAATCATTAAAATAGTTTGAACCGCCCGTTAGATCATAAACTAAATTTCTTAAGCTGTTATATTTATCCGTGCCATAATAATATTTGATAAGAGGAGCAACATTAACACCATTTTTAACTAATGATTTTCTAAAATGTTCAACATTCAATTGTTTGTTTAAAACTGAGTTAATATTTTCTGTATACATTTTTAAATTATTAATATTAATTTGTTTAGCATCTTTTAAATCAAAAACTAATTCAAGATCCTTTTTGTTTCTATCAATAAAGTTTCTAAACAAGTCATAATTTTTGTAGAATAAATCTTTATTATTGACTATTCGATCAAAAGCACCTCTTTTGTATTTGTCTAAATAAAATGAAATTCAGGAACAAAAATTATTATGTTTTGGAGCTAAAAGATCAAAAATATAAGCTCTGCTATTTTCATTATCAATATATAAATTTATAAAATTTTTGTAAGCAACTAAATATTTTTTATAACGGTTTTCATGCAAACCAAATTTATCATAAACATCATTGTTTAATTGCAATAATTCTTGTTTAATTGCTCAAGCATCTTTAATAATTTTATTAACTTCAGGATCTGAGCTAGCTTCAAAATTAGTTATCTCAATATCTGAAACAAATTTCTTTATTTTTAATAAAGATAATTCTTTTTCTTTAGAATTGATTTCAATTTCTTGAGCAGTATTTGGTTTTAAATAATATTTGTAAATACTGCCATCATCTTCTCATTCACCTTCAGGCACAAAATTAGGAACACTTAAACTTGGCTGTTTATAATCTTCTCAGTTGTCATCATCGTTTGAAGGTTTTTGTGGTTCAGCTGAATTATTGCAGCTTCAAGAAGAAAATGATGACATAAGTAATAAACATATAATTGGACCAAATGACATTAATATTTTTTTCTTCATCAATCCTCCTATTTTAATTATATTAAAATTTATAAATAATATTTAAAATTCAAGACTTTGAAATCAACAAAAATAAACTAAAACGCCTGTTTTAAAGGCGTTTTGGAAAATTAAGCTTCATATTTTTCTGGGTGTTCTTTCATCATTTTCTTGATGAATTCTTTTTTAGGCATCTTGCCATATTTAACCATTAATTCTTCACATTCTTTTAATTCTGCTAAGGCTCATTTTTCGCCTTCAAATCCTTGAACTTTTGTAGCTCCTTTTTTCTTTCAATTTTTATAGTTGCTAAAGAATGTTTCAATGTGCATTAAAAATGGTTCAGGTAAGTCTTCTAATTTTTTAATACCATCTAAACGATAATCATCAGCATGAACAGCAATTAATTTTGTATCTGTTTCTCCTGAGTCAATCATTTTCATAGCACCAATAATTCTTGCATTAAGAACAACACCTGGTGTAAATGTTTCTGGAGAGTAAAGTAAAACATCTAACTCATCGCCATCTCAATCTAAAGCTGTAGGAATAAAGCCATAATTACATGGGTAGACAAAATCTCCTCTCAAAATACGGTCTACATGAATATTTCCGTCAGCACGATCATATTCGTATTTTATGTTTGAATTCTTTTGAATTTCAACTTTCATTTCAATAATATTACTCATGCTATTATTATAGTTAAATAAATTAAAAAATATAATAAAAAGCAGCCTAAGCTACTTTATAATGTCGAAATAGTTTTGCCAACAATCATTGTTATACCAACAACAACAAATAAGATCCCGAAAAATAATGGTGCAAATAATTTAATTCTTTCTCAAGCTGGTAAATACATTCCTGTTGCTTCATAATTCGTTACTTTAGATTTTGGATTATTTTTTTTATATTCTTCCATTTGTTTTTCTTTATACAAATAAACCTTGTCTTTTGCTTTAGGATAAAAGAAAAAGCATGTAATTCCAATTACTATTAGTGATAATCCAATAATAAATAAAGCTAAATAAGGAGCATTTAATTTGTGTGTTCCTTGTTCGTAAAATGGGTTATTTGCTTTATAAAATACTGAGTTTAAAATCATTTAAGTCCTTTTTTAATGTTAAAAGTATAACAAAAATAAAAATATTTTGTACATTCTTTTATTTGTGTTTTTAATAATCAAAATTTAATATCTTTTATATAATAAAAGCATTATGACTAAAGAATTAAAAAAACCTAAAATAATTTTTATCGATTTGGATGGTACAACAATTGATAAAAGTAGCAAATTTAAAAAAGATATCAGTGAAATTAACCAAAAAGGTTTAGCTAAACTTAAAGAAAAATATGGCTTAGAAATTTTTGTTTCAACTGGCCGCGCAGTAGTTGAAAAAACAAAAATGTTGGCTAGAAAATGTGGCAATGAAAATAATTTTGTTGCTTGAAATGGGGCTAAAGTTTTAAAAGATGGTCAAGAAATTTTTTCAGCAGCTATAGATCAAGAAGTAGTAGAACAAATTTTTGAATTAGCTGAAAAATGAAAAATGACAATTATTATTAACTCAAACTTTAAAAATGGTTTGTACTCGAATTCATTTCTTTTTAAAGTTGCTTCAATTTTCAAAAAAGGCAAATGCTACAAACTAAAAGAATTTAATAAAGAAACTGAAGTATTTAAGTTACTTTTTCTTAATTTGTCAAAGAAAAAACTTCATTTATTTGCTGAAGAACTTAAAAAATTATGACCAAAAGATCTTAATGTATCTATATCAGGATTTAAAAATGAATTTTTAGAAGTTACATCATTTTTTGCTTCTAAGGGTAGTTCAAATAAATACTTAGCTAAAAGTTTTGGAGTAGATGCTGAAGATTGCTGACATGTTGGTGATTCACAAAATGATTCAACAGCTAGTGGACATATGGGTTATGTAATTGCTATGAAAAACTCTGTTCCTGAATTTAAAGATAAAGCAGATTTTATTAGTCCTTTTACAAATAAAAAAGGTGGTTTATATAAAACTTTAGAAATGTTACTTACAAAGTTTGAATAATTAAATTTAATATTAATTTATAATTTAATTTATGAAAGCATTTTTTAATTTACAACTAAAAATATATTTTAGACACGCATCAAGCTATGTCGTGCCTTTAATTTTCGGTATCTTTTTTATCATTATTAATGTTGTTCTTAAATGAAGTGCTGCAAGTCCAAAAGTTTTTGAAACTCTTTTATATTCGGTGAACTTCTTAACAAATATTAACCATTTTTCAATTTTTATGATTTTTATTGTTGCCACTTTTGTGTGTCAAACTGTATTTTATAAATACAAAAGAGAAGGTGTGGATTACATTTTATATTCAAAACCTTTAAGTAGAACTCAAATATATTTTGCTAATATTTTTGTATCTGCAATTGGTCTATTATTTTCAATAGCAATTATCGAATTAGGTTATTTTATAAGCAGATTGGTTTATCCAACAATTATTTGAAAAGAGGTCTGAGGTGCTTCAGGTGCTCACTTATTAGCAATAACACTAACAGCTCTTTTTGCTCTAGGAATTGCTAGCTTAGTACACTCAAGTGTGGGTTTAAGGATGTTTCAAATTTTAATAGGTGCTATACCTTTTCTTATAACAATTATTTTCGGTGTTGTTAAGTTTGCTTGATCTGTAGATAACATTAAAAGCTTAACTAAATCAACAAATAATGTTATAGCGACCTTGCCTTCTAAAGAAAAAGGCAACAAGAAATTCCTTGAAGAATTAAAAGGCAGATTCAATGAAAATCAACCTATCTTGATGAGTAGATACAACGATGACTTAAAAAATACTTTATTAAATATTACAGAAGATTCAGAAGATATCACAAAATATATTGACAATACTCATAAGTCAGTTTATAACTATTTATATTGATTAAACATTGAAAACTATTTCTCATTACAAAGCGGTTGCTTTGATCATCGTATTAATTCATTTAACAAAAAAATTAGTTATTACAAAATAAATCCACTTAAAAGTGACGGTACTATAGATTATCGAATGTATGAAAAATATGGCTATCATTTAGACAACAATATTAAATTAAATATCTGAAATGTTGTTTATCAAGATAACAATAAAAATTCTATAAAAGAAGTTAAATCAAAAAATCTTTTTGGTGTTAGTTATGATGCAAATTCAATTAAAAATAAATTAAGTAAAGGCTACTTAACTAAAGAAGATTTATATGAAAGTTTTTATAAAACTTATAAAGAAGAAACAATTGAACCAATGATTGATAAGTTAAAACTTTTTGAATCAATATTTTTAGATAAATTATATGAAAATATTCAAGAGATCAAATTTAACAAATTACCTTATGTTATAGATTACGAGGGCAAAAAGGTATTAGTCTTATCAAAAGTATTTCAAGACTTTAACAAAATATTAAGCTATGTTGAAGAACATCAAAAAGATAAATACAAAAACTTAAAAATTAAAACGTATATTGCAACATATTATCCACAATGAATCGATTTCTTTGAACAATTTAATGATGAAAAAGTAGCAAAACTTAATGAATTTTTTGTTGCTGTTGCAGAGCTACTCACAATACAACCTAAAAAACCAAAATCTAATTCAATTGGTGAAAGAATTAGATATGAAAAGAAACTTAAAATATATCAAGAAAAATTAGAAGAAAACAAAAAACTTTTTAAAAAAGTTGGTTACTTAATGACTCTTATGTATGGTTTAAATAAAGAACAATATGTTTTAAGTTATCAAGCTTCACCTAATAATTTGGATGAATTAAATGAATTAGACAATGCAGGTGGTATTGAATTAATTAAATTAACACCACTTGAAAGAGATTTAATTGTTGTTACAAGAAAAGATCACATGACAGTTGGCGGATCAATAATTTTATCAATGTTAGCTGCTTTTGCTATGATGGCTGGTGGTTGAGCAATATTTGTTAGAAAGAATTTTAAATAATGAAAACACTAGTTAAAAAAAGTAAATGAAGAATTTATCTTGAAAAAAGAGCAAATTATAAAGCTACAATCAAGAGAGCTAAAAATTATGAATATCAACAGCCTCAAGAGGATGTTGCAATTTCAATTCAACATTTACATAAATTTTTTAAACTTGGACATGGCGATTTTAAAACTGCTTTAGACGATGTTTGTTTTGATGTTAAAAAAGGATCATTTCATGGTTTTATTGGTGATAATGGTGCCGGTAAAACCACAACTATAAGATCAATACTGGGATTCTATCCTTCTAAAATAGGCAAAATATTTATCAATGGTATTGATTCAACAAACAAGAAAGCAAAATCATTTATTGGTTATATTCCTGAAGTTTCTCTTTTTCCTAGAAAAGTAAAAGTAAAAGAATATCTTTTAGCATTTGCTGAAATGTCAAATTTAAAACCTGAAGAAGCAAAAGAAAAAGTAAATTTCTTATTAAAAAAATATCACTTTGATGGACCTGAATTTGAAAAATCAGCAGAGCAACTTTCATCAGGCCAAAAGAAAACAATTTTATTAATGCAAGCTTTATTAAATGATCCTGAAATTTTAATTATGGATGAACCTGCTGCTAATTTAGACCCAAGTGCTAGAGTGATATTTTATGAACAAATAAAAGAATTAAGTTTACAAGGAAAAACTATATTTATTTCTAGTCATATTCTTCTAGAACTTGAAAGATATATCGATTCTTTTACGGTGCTTGCTAAGGGCAAAGTACTAGACAATGGAACAATAAAAGAAAAATTGCACAATGCAAAATATAACAAAAAAATAAATACAAATGATAACAATAAAATAGTGGAATATTTAATTAAAAACCGAATTGATTTTATTGAAAAACATGATTGAATTAATGCAAAATTAGATGAAGAACAAGTTAATAAATTTCTTCAATATTGTTTAAAAAACAATATTCAAATTTATAATTTAGCTGATAACAAATTAAGTCTAAATGAACTTTATTTTAATGCCGAAAATGTTGAATAAATAATGAATAAAAATAGAACTGAGAGATTAAAACTCAGTTCTATTTTTTTACGAAAACTATTGTTTTATAGGATTATTAGGATTTTGTGGTTGTCTTTGAGCTACATTTATGTTTGTATAATTTTGGTTTGGATAATATGGGTTTTGTCTAGGAGGATATTGTGCAAATCTTGGATCAACATAGCCTTTATTTGGAAAGTTTTGATATTGGTTTGGATATTGAACATTTTGATACATATTTGGATTTACTTGGCGAAGTCTAGAAGTTTGTTGGTATTGATCTTGAGGTTTATTTAAATTGAATTTAGGTTGATTTGGTTGAGCTTGTTGTGCAGAAGGATTTTTAACTTCTTCAACTTTAATATTTTGTTCTTCAACTTTAGTTTCAGGAACAACTTCTTCCTTAATTTCTGTAATTGTTTCTTTTGCATCTTCTTCTATCTTTTGAGTTTCAACCTTTTGATTTTTCTTTTCTTCAATTTCAGCTTCTTCAAAGTTAGCCAAGTTTTCTAAATCAACAACTTTTTGAGCTTCTGCTTGTAATTGTTCAAGATTATCAACATTTTGAACTTCGACATTTAAGTCAACTTCTTTTTCGCCACCATTTTCATCATCATCTGAATTATCTAATTTGATTTCACTTGAAAGTTGATGACTATCATTCATGTAATATTGACAGTCTAAACCTAAGTATGATTCTTTGAATAATTGCTCAATTAATTCAAGATGTTCAATTTGATATTTCTCAGTAATTTCTTCTCTTGCTTTTTCAAAATTAATGATATTTTTATTAGATTCAACTTTGTTGTATTCATCTTGTGTGAAGTCCATTAAGATTTCTCTATTTATTACAACTTCTGAATTTTCTTTATTAGTTAATGTATCAACAATAGCATTTGCTACAAATTCTCTTGATCAGTAGAACGATTCAGTTAAATTCATATCTAAGTTTAATTGTGAACATAAATTAAAGGTTAATAAATATGCAAGTGATTTAACTGTAATTGCTTTACCATCTAATTTAATGTTGGCAAATACTTTTTCTTTGCTTTCATTTTCCACTATTTTAAATAATCTATTGTATTGTTGTTTTTTAATTGAATTAAGTTTGTATAATCAACCTTTTTCTTCTAATAGGTTGCTGTATTCATGTGAGAATAAGTTTTTGTAAATTAAGAATGCAAATAGTTTACTTCTATTGTTTTTCAATGCTTTACGAACATAAGTAATTAAATATCTACTTTCATTAGGTTTTTTTGTTTTAAAGAATATTTCAAGATCTGAATTATTTCTTAATAAATCTAATTGACTTTCAATGTAAGGATCAAAACCAATTTCTTGATAGTGAATTGAACAACCTGCATAAGCTAATTCTTGAGCTATCTTGTTGTTTTCACCTAATGTTTGTTTTAAAGCTTTGAATGCATTTAAAGCTAAATTGTTTTGATTTTTGATTTTGCTAAATACATTATTTTGCAATAAACTGTTTACATATTTAACAATTTCTTCAAATTTATTGTTGTAATCTTTTTTGAATTCTTCTGCAATATCATCACCCATTGTGTTAGTGCTTCTGAAGTTATGGATATCTGAGTACATATTAAAATCATTAATTAATACTTTGTTTAATTTAACGTCTTCAATTTGTTTTATTCAGCTTAATTCTTTAACACTTAAATTTGATTGTGATAACAATTTATTGATTTGTTCTTTTTTGTATTCTTTGCTAATTGGTAAAACAGTTAATGAAAAATCAAAAATATTACCTTTGTCTTCTAGTGATGCAAGTGTGTTGTCAATTACATAGAAGAATTTAATTGCTTTACATTTTTTAACATAGTATTTTGATGCATAAAATTTAGCAATTTGTTCTTTTTTATTTTCAAGTTTAGCTAAAATTTTTTCAATTCTTTTTTGTCTTCTTTTGTTTACAAGTGTGTTAATATCTTTAATTCTTTTGATTAATTGATAATCTTTAGATTCGCCTAAATCTTCTATAATCAAAGCATCTAAGCCAGATTTGTCAATTAAGTTAACAAGTTTTTCTTTATCTTCAATAACTACTATTTCTTTAGTTTCTTGAACTACTATATTTTTTGATTTTTTTAAGTCTAATAAATAGTTTCTACCATAATAAATAGCAATTGCTAAGAATCCAACTATCATTCCAAATAAAATAAATGCAACTGGTAAGAAGAATCCTAAATGGTTTAAATCAAATGATTTGCTTCAACCATGAGCAATAATTTTTCTTGCACTAAAGCTAAAAAATACTACTGATAATAAAATGAAAATACTTGTTAAAGCTGTTCATAATACAACATGTTTTACACTTGTTTTATTATTTGCTTGATTAACATTCTTTTTAGTAACTTGGCCTGAAATTTGATTAATGATTTCTGATTCTAATAATTCATTATTTGTGTTTGCTAATGAAACATTAGCTATATTAAAATCTTTTCTACTTTATTTATATGTTTCAATAACATTTTGATTTTGTCCATCTTGAGAACCTATAATAGCAATATTATTCACTTTGTCATTGTTGTTACTCATTGCAAAATCAAGTGATTTTTTATATAACTTGAAAGCATTAGAATTTTTCATGTCTTCAGTTGAATAAAGCTCTATAGTTTCAATAATATGTTCTTCATTTTGAACTTTATTTTGGATGTTTCCATTTCTAATACATTGGTGATTTGTAAAAGAAGAAGAATTATTATTTTGTGACATCACCACCTCCTTATATTAAGTAAAAAAATTATACCTTATTAATTGTATTAATAATTAAATTGAAAAATAATTTATATGCTTTTTTAAACAAAAATTATTCTAGTTTGTCCAAAAATTATAAATTTTTGACAAATAATACTATTTGATAAGACTATTGGCAGAATGTAAAATTTCAAATAATTTTTTTATACTTTTTAATAATAGTTTGCTTATTCTTTGGTATTATATTTACATAACTATTAATAGTAAACTGTATTGTATTTCTACATCTTTATAATAATCCTTCATAATAGAAGCATAAAGGTACTTTTAGCAGTTTTTTTAAACATTAAAAAAGGAGATCAAAACATGAATCCTACAAGATTAATACCTATTGGTGGAGTTCAAGAAATTGGTAAAGCTGCACTAATAGTTGAATACAAAAACCAAATATTTATTATTGATGCAGGTATCAAATTTGCTGATACAGCTTTAACTGGTATTAAAGGTATTATTCCTGACTATACATATTTGGCAGAAAGATATAAAAATATTGAAGGTCTTTTTATTACTCATGGTCACGAAGACCACATTGGTGGTGTTATTCATTTAATGAAACAAGTAGCAGTTAAAAAGATTTTTGCACCTAGAATTGCTATTCAATATTTGAAATTAAAATTCGATGAAAATAAAATTAAAAACAAGGTTGAATTTATTGAAATTGAAAAAAGTGCTGTACACACTTTTGGCGAATGTTCAGTAGATTTTTGAACAGCTCAACACTCAATCCCAGATGCTTTTGGTATTAGAATCAAAACTCCAAATGGTTCAATAATGTGTACCGGTGACTTTAGATTTGATTACAACCCTATTGGTGAAATTTATACTGATTTTGCTCGTTTAGATCAAATTGGAAAAGAAGGCCTAACTGTTCTTTTGTCAGACTCAACTAATGCTATGCGTCCTTTCCACTCGCCTAGTGAAAATGACATCTTAACAGATATTGAAAAATACATGCGTCAAGCTACAAGAAAAACTATTATTACAGCTTTTGCTTCAAACTTAACTAGAGTTACAGCAATTATTGAGTTAGCTGTTAAACTTGGCAAAAAAGTTGCAGCTTTTGGTCGTTCAATGGTTCAAGGAATCAAAATTGGACGTAAATTAGGTTACATTAAAGCGCCTGCAAGTGTTTTTGTTGATAAAAAAGATGTATCAAAAGTTCCTGACAAAGATCTTGTTATTCTTACAACAGGTTCACAAGGTGAACAATTGGCTGCTCTTTCAAGAATGTCTTATGGTAAACACCAAAGCGTAAAAATTGAAAAAGGTGACATGATTATCTTTTCATCAAGTCCAATTCCAGGGAATAGAATGGTTATTGAACTTTTAGTTAATAGACTTTCAAAACTTGGAGCTATTATTAAAGAAAATGGTGTTGATGGTTACTTGCATACCTCAGGTCATGCTTACAAACACGAACATGATAAAATTTTCCAACTTACTAAACCAAAATACTTTTTACCTTACCATGGTGAATTCAGAATGAGTATAGTTCATGGCCAAAGCGCTATTAGAAATGGAGTTAAACCAGAAAATATAATGATTCCTGAAATTGGTCGTGTTTACAACATGATTAATGAAGAAATTACACAAACAGATGAAAAAATAGATTGTGGTCCTATTTATATTGATGGACACACAACCTTGAATTTAAGTGCAAATATACTTAAGGAAAGAGCTAAATTAGCTGATAGCGGTTTTGTAAATATGATCCTAAATATTAACAAAAAAACTAATTCAATAGTTGGCCGTCCAAGTTTAATTAGTCGTGGGAGCTTCTATGTAAAAACTTCGCTTGAATTAGTTGAAGAAGCTAAAAGAATTGCTCATGGTGCTGCTCTTTTCTATATTAAAAATACACCTGATTGAAATATTGTAGATTTAAAACAATTGATTATTGATCGTCTTGAAAATCTTTTCTACAAAGAAAAAAGAAGAAGACCAATTGTTGTGGCAACTATTCTTTTTACAGATGAAGAAAATGAACCAGCATTTGCAACTTCTAAAATTAAATTTAATGTTAAGGGTACAAAAGAAGATGAGAAAAGCAAAAAAGCTACTGCTGCTCTTAAAGAAGCAAAATCAGAAATGTTTGGTGAAAACAGTGAAATGTCAGAAGACATTCATGATGAAGAAGACGAAGAATAATGATAAAAAAATTAGCAATTTGATTGTTGCTAATTTTTTAATAACACTATAAAACAGTGCTTTTGGTCAAATAAATGATTTGGTACACCGTGCAGGGTTCGAACCTGTGACCCGCTGGTTAAGAGCCAGCTGCTCTACCGACTGAGCTAACGGTGCTTAAGTGCATTTATTATATATCAATTGTTTTTTTCTCAAAATATTTTTACAAAAACAATGAGTAAAAAGTGCAATTTCAATAGAAATGAAAACAAATAAATATAAGATTTTTTGAATGGTGGATCTCACTTTTATAACATTTGTTGCTATTGAATAACTTAACATTTTTTGTCATACTATATAATTAGTTGCTTAAAGTAAGTATTCAAGAACAGTGCATTCATTTCATGACAGTTGTCAAATACTCTTTTGGTGACTTTTTTATAAAAAAGTTGAATAAAAAAATGCAATAATGCACTTTTTAAACTTCAATTGCTTCATTTTCTTCATCAACTAATTCATTAACAATAGAATCTCTTTTAGCAAGAATAATTCCTGCTACTACATAGAAAACAGGGACAAAGAAAATTCCTAAAATTGCTAAAACATAAATGTAACGATCTTTTTTAAGAAAACTTAATTTTGCTGCTTGAATTGAACCAACAACAATAAAAGAAATGCATAGAGCAAGAGTAATAACTAATAAAATAATGTTAGTAATTCCAGCCACATAATTGTTATTTGTCGCTTTTATTACTGCTTCTGGGATATTTTTTTGTAATCCAAAAAATGAGATAGTTAGTGTTAATAATAAAATTGCTGCTATAGCAATTGAAGTTAAAGTTGATTTAAAACTTTTTTTATTCATTTAGTTTCTCCTTATTATTTACAATAAAATTATACAAAATATTATTTCATTTCTAAAAGGTTAAATATGCAAAATTAATTGTTTTCATTTTTTAAACTTTTGTATATAATAAAAAAGCAATTATAAATAATTGCCTCTAACTAGTGGGAGAGAAAACAAAAAGTATCATTAGTACCACACTTATCGAAAGGATATTACAATGGCAAAATCAAAAGCTGATATTGTCCGTGTTCGTAAGCTACAATTCATCGCTGGCCAAGCCAAACCTGGTCCAGCTTTAGCTGGTGTTGGAATCAATATGCCTGATTTCACACGTGCTTTTAATGACGCAACACGTGATAGAGGTAATGAACCAGTTCCTGTTGAAATTACCGTTTACAAAGATAAATCATTTGACTACAAGTTATTTACAGCTCCAGCTTCGTACAAATTAAAGCAAGCTGCTAAATTAAACAAAGGTAGTTCAAACTCAAAAACAACAATTGCTGCTACTATTACAAAAGAACAATTGAGAGAAATTGCTGAATACAAATTACCAGACTTAAACAAAAATGATGTTGAAGCTGCTATGAGAACTATTGCTGGAACAGCAAGACAAATGGGTATTATTATCGAAGGATTTGATAACGTTGCTGCTAAAAAAGCTGCTGCTGCTAAAGCTGCAAAAGACAACGCAGCTGCTGCAGCTAAAGAAGCCGCTTTAGATGAAGCTCAAAACGAACTAAAAGAATCTAAAGGTAAAACCATTGATGTTAAAACAATTTCAGACGAAAAAGCTGCTGCTAAAGAAGGAGAAGAATAATGTCTCTTAAAGGTGGAAAGAAAATAAGAAATGCTCGTGAATCATTTGACAAAACAGTTGCATATGATTTAAAAGAAGCATTAGAAATTGTTAAAAGAACATCATTTACAAAATTTGTTCCTACAGTAGAATTAGTATTCAAATTAAACCTTGACGTTAGAAAAGCTGACCAACAATTGCGTGGATCAGTATTGTTGCCTAACGGTACAGGTAAAAGCGTTCGTGTATTAGTTGTTTCAAACAATCCAGCAATTCAAAAATCATCAAAAGCTGCTGGTGCAGACATCGTTGTTGATGGTCCAGCACTTGAACAAAAAATTAAAGAAGACGAATTTGACTTTGATGTTATGGTTGCTGACCCTTCAATGATGCCTTTATTAGGTAAATATGGTAAAAAACTTGGACCTAAAGGTTTAATGCCAAACCCTAAAACAGGTACAGTTACACCTACTCCTGATAAAACAGTTGCTGAACTTAAAAAAGGTAAAGCTAACTACCGTACAGATAAAGCAGGTATTGTACATACAATCATTGGTAAATTAAATATGACAACAGACCAATTAGCAGAAAATGCTCAAACAATCATTAATTTAATTAAAAAACTTAAACCAGCTACAGTTAAAGGTGCTTACATGCAAAACTTAGTTGTAACATCAACAATGGGTCCTGGTGTTAAAGTTAAAATTGAAAAATAATCCAAACAATTAAACTTCCTATTTAGGAAGTTTTTTGTATAAAAAAATAAGCCAAACTTTGACTTATTTTATTGTTTTATAGTGCTTTTAATCAATTTTAATCTTTAAAGTTTCCTTAATTACTTTAACAAGTTTTTTAGCTTTTTCCATGTCTATAGTATTAGGAATTCCAATTCTGACTTGTTTACTTCTTCTATTTCCACGACCTAATTCAAAATAAATATTGTGATTATATTCAATTTTATTTCTTAACTTAATTGCATCAACACCTTCTGGAACATTTAAAACTAAAAGTCCAATAGTTATATTCTTTTTAGGAACTACATCCTTAAATCCAATAGCAATTAATTCTTTTTTAATGTAGTCATATATTTTGAGTTTATTATTTCTTATATTCTTAAATGTTTGGTTTTTGATAACTTTCATTGCAGCATTCAAAGCTAATAAAATACCTGAAGCTGGAGTATAAGGAGTTGATTTTTCTCTTTCATAAAAATTAATATAAGCTGATAAATCAAAATAGAATTTAAAGTTTTTATTTGCTTTAACTACTTGTTGAGCTCTTTTAGATAAAACAACACAACTTAAACCAGGCATTACACAAAATGCTTTTCCGCTTGTTGCTATAGCAACATCTAAACCTCAATTATCAAACTCAACATTTTCATGAATAAATGAACTAACTGTATCCACAATAAATAATGTATCTTTAGCATATTTTTTAACTACTTTTGAAATAGCTTCAATATCATTTAAAACACCTGTTGATGTTTCATGATGAGTACAGAAGAATGCATCATATTTATTTTCTTTAAGTTTATTTTCTATATCTTTTACTTTGAATGCTTCACCATCTTCATAATGAATAACATCAAGATTATTAGCTCCAATTAATCTTTCTAAAATTATTTGGAATCTTTTACCAAAGTCACCTGCTTCAACCAATAAAACTTTGTTTTCAGGACTAATAAGGTTAACAACAACTGCTTCCATAGCCCCAGTTGCTGAACATGTAATAAATAATGGAAGTGCTTCTTTAGTTCTAAAGAATTCTTTAATTAAATTAGTTGATTCTTCCATGTAGTGTTTGAACAACTCACTTCTATGATGCAACACTGACTCAGATAAAATCTCAGAAACTTCTGGATAATTATTTAATAGGACCTGGTGTAAATAATAAAGTAGAAGCGTGGTTTGCATAATGATGATCAATATTAATTAAGTCTTGAAAATTGTCAAGTTCTTTAATCAAATTTGAAGAAATTGCTTCCAATCTCATTTTGTTTTCTAAAGTTCATAAAAATGTTTCAAAATATTGTTGCTTATTTCCTTGTTGATTAAATCATGCATCAACTTTTTCTTTTGCTTCATTAGCTAATTTTTTATTTAAATATAAAATACCAATTCAGATATTTTGATTATATGCATCTTTTTCATTGTGATGTTTAACTACATATCCTTTATTATTTGTATCAAAAACTCATTCCGATTTGTGTTTTGTACATTTTTCAGTAAAACAAATTGAATCTTCAGAGTTTTCAATTATTTTAATTATTTCTGGAAAAATATTTTTTTCAATATACAAATCACCTTCAATATATAAAGTGTCTCCAAATGCATTTTTGGAAACAACATAAGAATAAATGTTGTTTGCTTCATCATATAAATCATTTTCAATTAATTTGACATTATATTTTTCTTTAATTAAATTAAATTGGTCTTTCATATAACCAACAACCACAGTGATGTCATTAATGCCTATTTCTTGTAAATGCTTTATGTTTCTTTCAATCATTGATTCATCATTTACAGTTATTAATGGTTTAGGAACATAATGAGTTAATGGGGTCATTCTAGTCCCCTTACCTGCTGCAAAAATAACAGCATTTCTAACTTTTGCCATAATTACCTCCAAACATTTATTAAATTATATATTTAAAATAAAAATTGATTTATATAAATATAACAAACAACAAAAGAAAAGTTAATCCTCCTAAAGCAACACTATCAAAACGATCTAAAATACCACCATGACTTATTAAAATTTTTGAATAATCTTTTATCGCATTTATTCTTTTAACATATGAAAAGTATAAATCACCTAATATCGAAAAGAAAGGCATTAAAACAGCAATTGATATTTGTGCTTCTAATTTTCCATAGAATAAATTAAGCCCTAATATTAATGCTAATCCAATTCCAATTCCACCAATTAAAGCAAAAATAAAACCTTCTCAAGTTTTTTTAGGTGAAACATTTGGAGCAAATGATTGTTTGATTCATTTTTTGCCTGCTAATGTTCCTCCAACAAAACCAAAGATATCACAAGTAGAAGGAATAACAAATAATAGGATGGTATATCTTCAATCAAGTATAATGGTTATTTGAATCAATTTTGAAGTGTTGACTATTACGTATAGAGAAATAAACATTAGTATTCCTCTAACCAAACGATCTTCAAAACTCATATTATTTCTTGTTATATATTCAATAATAATAAAAGCAACAGAAATCATTAATAAAACAACTGGTGTAAATCATTCTTTTGCTATTTCTTGAATAAAACTAGGATGAAATTCTAAAGGATGAGAAACAAAAATAAACTCAGTAAAGTTATTAACCGGTAAAACTACAGTAGGTAGTATAAGCATAATAAAAATAGCAATAACATATCATTTTAAATGATTAGCTTTGAAATATTCATAGCATAGTATTCCTATCCCAATAGCAGTAAAAATATATGAAACTATTCTAGCTTCATAATGATTTTTTCCAAAAATTGCTAAAGGTACCATTCAACTTAGCAATACTACTACGAAAATAAAAGCAGGTAGTATTCTTGATTTAAAAAATTTCATGTTAATAATTATACATTTTTTTAAAATAAAAAAATATTAAAAGTTTGTATGAAATATTCCTTTTTTATAGTCATTTTGGTCAAAAAATATCCTTTGCATTAAGCAAAGAATATCTAAATTATTATTTTTCTTTAAAAAGTTCAATATCATCAGGGTGAATTGGTTTTTGATTAACTTCAACTTTTGCAATTCTTCCTTTAACAACATGAATAATTCTATCAGCCATTTGCGCCATAACTGGGTTATGACTAACCATAACAACTGTAGCGCCTGTTTCTTTATTAATATTGTAAAGAAATCTTAAAACTATTTTTGTTGTTGCTTCATCAAGAGCACCAGTAGGTTCATCAGCAAAAATGATATCTGCATTTTTAGCTAAGGCTCTCATAATTGAAACACGTTGTTGTTGACCACCAGACATTTGAGCAGGGAATTTTGATATTTCTTCATCCATTTCAAATTTCTTAAATAACTCAACAACATCAACTTTTTTAGCAGGATTTTTTTGTAAGTAAGCTCCCGTTTGAACATTGTCAAAAGCATTTAAGTTTGCTAACAAGTTATAGTTTTGGAAAATGAAACTAACATGTTTTCTTCTAAATAGAGTTAATTGACTATCAGACATATAAGGAAGATTATTATCGCAAGCAATAACTTGTCCTTTTGAAGGTCTATCAAGTCCTGAAATAAGGTTTAGAAGTGTACTTTTACCACCACCACTAACACCATAAATAAGCATTAGCTCACCTTTTTTAATTTCAATTGAAACATCTTTTAAAACTCTTGTAACAATATTACCATTAACATAATATTTGCTAACGTTCTTAACTTCAATAACATTACCTTCTTCATTTTTTAGGTTTTCATTGCCATCTTTTTTTCTTTTTTTCTTAGCTAACTTAGTCAATAATTTTCGAACTTTTTTAGGAACAATAATATTCTTTTTAGAACTATCTCTGTCTAAAAGCTCAAAAACATTGCCTTTAGTAATTTTGATATTTTTGTCAATTTCTAATTCAGCGACTTGTTCTTCAAGAAGTTTTTCTTCTTCAATAATAGCTTGTAAAAGACCAGTTTCTTCTTTTTTATTTTTTTCTCTTTCAGCCTTTTTTGCAGACTTTTTGGTCATTTTTTTGGCTGTTGATTTTTTAGTAGAAGTTTGATCTTTTTTAACTTTTTTTTCTTTGTTTTCTAATTCTTCAGACACACTATTTTCCTTTCAATAAATCTACTGGTTTCATTTTACTAATACTAATTCAGGTAGCAAATGATGTAATAATAAATATTCCAAAAATTACTAAAGCTGTTAAGAATATATGTAGTGGCGTAAGCACTAATGGCAAGGCAATTGACGAGGAGGCCAGCAAGAACTTGTTAAATGCAAATATCATCAGTATTACTATTGGTATCGCCAGAGCGATGGCCAGAACTATAAATGGAATAAAGACACTGAAGAACATACTAATTTTTTCTTTTTGTGTATATCCAAGAATAGATCAAATAGCAATGTTTTTCTCATTTTCACTAATCATAATTGTTGACATAATAATAAGAATAACAAGTGAAATTATGAAACTAATTGCAATAATTGCAATACTAATTGTTCCTATAGTAGAACCAATTTGGGTTGTAAATCCAGCTTCTATATCTTTTGAATCAATACTTGTACCTAAAGCAATATAATGTTTAGAATCATACAATTTAGCATAATTTTCTATTGCGTTTTTAGGCGCATTTTTAATTTGATCATATTTAGCTTTATTAAATGTTAATTCAGTTGAATCTAATAAATTCATAATTTGATCTTTTGTTAGATTCAATCTGGTTTTTAATATTCCAGTATCTGGATTATAAATTTGTTCAAACATTGATTTAAGTGTGTTGTCATCGATACCGCTTAAATCAAAACCATCATATCCCGATCAATAACCACTTAATGAATAAAGCGAAGTTGAATCAAGAACTTGGCTAGGCGCTGGACTATTTGTCATAACACCATTGAAGGCTTTAAATCCAACAACATTTGGAAGTTTGTCTAAGCCAACTAATTTATTAGCTGCATCAACTGTTGTTATTAATTCATTATTAATGTATGTAGGGTTAATACCAACAACTTTAAATGTTACTTTTGGATTTTTTAAGTCAACATTACTTCTTAATGATTTATCTATTTTTGACAACACTTTGTTTGCATAACGTTTTGTATGGTTAAGAACTTCAAATTGGACTAAATCGCCTATATTAATGTTATATTTCTTCGAAGTAACTTCATTTACAACCAAAGGATAAATGTTGTTTTTGACTTTATAGTTATATAAATCATTGTATAAATTATTACCATTTTTATCTACAAGTTTTATGTATTTACTATCTTTTTGATAACCATAAATTTTGAAGTTATTATCTTTGTTTGCAACTTTTACATAAGTATAAGTTTCATCATGATTTGGGTCAATATAAACACCACCAAAACTAATAAAGTAATCTTGAATAGTTGGACCACTTAAATCACTTTTATCAGTTAATCAGTAATCAGATAAATTGGTTGGTTTCTTTTCTTTAGGTTTAATTAAACTTGGATTTTCTTTTTCGGCTTTAATTTTTTGAGCAATTTTAGTATAACCATTGATTAAGAGTGTTCTAAATTCATCACGATATTTATCGGTTGTTATATTTACCATTTGATAATTTGCATTATCTTTATCTCAGTAAGCATATTTGAAACTACCTTGTTTTTCATATGGTGAATGATAGTATTTAAAGAAGCTTAATTTTTCCTTATTAGCCTTATTTACTAAGCTTAAAGCATAAGTATTAGGATCTACAAAGAATTCTTTATCTTTGTCTTCTTGAGTTCTTTCTAACTCATAACCAACACGGTCTCTTATCTTATCAATTTTAGCTTTTTGAGTATCTGGCATACCATTGTAAGCTACTTGTCAAGGGTCAGCGGCTACACCAGCTGCAACAGTAACATTAACTGAGAATTGGCTTAAGATATGTGAGTCTAAATCTTGAGGACTACCATTTTTAGTTCCTTTATTAAGCACACTAGAAGCACCAGGTTTAAAGTAATCTCCTATTTCTCTGTTACCTTCATTTATATCACCAAGAGGAGTATAAATGTTGTTATATAAATTATCAGGATTAAATGGTTTGAATGCTCCACCTTCAACTGTTGGACTTTCTAAATCAACTTTAAATGTATAACTTCTATTTTTATAAGTATCATTAATAGTTCTATTAAAGACGTTTGTATTGGCTACACCAAATAAGGTTGCGATACCTGTTAACATAACACTGACTGTAAATGAAATTAGTTTTCAAAAACTTGTAAATGCCAATGTAAATGAGAAACGTTTTTTAACATTAAATTTCTTAGTTGCTTTTTGATAAGTTTTGAATAATTTTGATTGAGGTATTTCATTAACTCCACTAATTAAGTCTATAGCTTTATGTCTTAAAGCAAATAATGAAACTATAAAGATCAATAAGCTCATTCCAATAAATGGTAAGAAGACAGTGAAGAATAATGTAAAGAAGTTAAATGAAATCGTTTGTTTTGGCAGTGTCCAATAACTACTAAAGATGTTCATAACCATCAGTTGTAGTTTATAACCAATTACATAACCTAAGATTCCACCAATAACTGATGTAACTAAAGCAAACACTGTAAAGCTTAGTGATATTTGTCATGGACGATAACCTTGGGCCACTAAAATACCAATAACTTTGTTTTTATTTGAAATATATCTCTTAATAATAAAGATAGTTGAAATAGCCACAAGTGTAACGAATACTGAAACTAAGGCAATAGTAGCTGATGAAACAGCTTTAATAATTCCTTGAACAGTTGTTATTCTAAGAGACCTTTCAGGGTTAATTGGATCAATTTCATTTGCTAAGAAAACTCTTTGAAGTTTGTTACTGTCTGCAATTGAGTTATCTATTATTTTAGTAATGAATTTTTTAAGTTCATTATTATTCATACCAGTTGCTGCTGAGTTTTTAACTAAAAGAGTTTTCTTAATAACATTTCCAATGTAACCTGAGTGAATTCTGTCAAAACCATATTTGTTAACATAAACTAAGGATTGGTTTTGTGTATTAACTTGTAAGTTATTTTCATCAATAACTGGGTACATATAGTCGACTGTAGTTTCTTGACCAATAATAATAAATTTGGTTCCATTAACATTTAAAATGAATTTTTCATCTAATTTATCTAATAATGATAATAGTTCAATAGGATCACTTGGAATTTTACCTGTATAAATTGCTTTATTATTTTTTGCTAAGTATGCATAGTTAACTTTAGCTAAGTAACTATTTGAATTTGAGAACACAATGTCCTTCATTTCTTTAGGTTCAACTATCGTGTTCATTGCTTCATTAACAAATTTAATAAAATCAATTCCAAATAATGAAGAGCTTGTATCTTTTAATTTATCAAATGTAGCTCCATCCATTACTAATGTTTGTTCGTCATCTAAGTCAAGAGGAATTAGTGAGGTATCTTTTTTGCTAAATGCAGTTCCTAACATGTTAGTTAAGGCATAAAATTTGTCTTCGCCTTTTCTAACAATTTTTTCGAAATCATCTATTTTATTGAGCAATAAGTATTGTAAGTTAGCTTTAACTAAACTAGCATTTGTTTTTTGACTTGCTAAATAAGATAATGTAATTTCAGGGAAAGTAAATCTTAAAATAGGATTGACTAAGGCTTCACTTAAACCAAAAATTTGTAATGGTACATTAAGATTTAATGAAGACATAATGATTTGAGCTTGGTCTTTAATGTCATAAAAATCTTTGCTTAATAAATAATTGTTCAAGAATTTTTGAATTATTCCTAAATAAATACCATCAGGATATTCAGAAGAATTACTGTAAAGTTTGCTTGCTGTAATATAACCTAATTGTTGTAATATTAATAATTTATTATTTTGAATTCATTTTCTATATATTTCATTTTTAGCAGCAAATTGTTCAACTTTGTCATTTAAGAATTTGCCATTAACATCATAAGCAAATAAGTTTGATTTTTCGGGATTTTCAAGTAATTCTAATAATCCATAGTTAGTTACACCAGCAAAAGGTATATTGTCATTTGTTGACATTTTTTTGTTAATGTCAAATATTTCAGGTTCCAATGCTAATGCAATTAAATCTTTAACAAATCTTTTTGCTTCTTCATGATTTGCATATTTATTCATAAACATTTGAATTCATGGATTATAAATTGAAAATGTTTGAGCTCCTAATGCATATTCATTTTGAACAACTGGAGTTGTTAATGAAGATAATAATTTTTTAATTAATGATCAAGTCCCGTTGTTTTCAAAATTTTTTAAATCATTTAATAAATCACTACCTGTTTTATTAGCATCATTACTTATATATGAAGTTCCACCTTTTGTTTGTTTTATGAACCTTAATATTTTATTAATTTTTTCTAATATGACTGTTTTATAGGTTTTTGTGACAAATAAACCATATTGTTTAAGTAATCTTTTTTGAGGTTCTTTTAAGTAATCTAAATTAATTTGAGTGCCTTCCTCTTTAAGACTTTCAATTGATGATTTAAGTGCTAAGAAGTCATGTTCAATTTTGTAGTTATTGAATTCATTAGCTTCTGTATTTGATAAACCTGAAGCAAAAATTTTTAAGAAATCAAAGAAACCTACTTTTGTGTCGTCATTATCTGGAACATAAATTTTTACACTACTATTAGGTAGTTCTATTTCTTTATATTTGTCAGACAAATTGAACATTTTGATTAAGTTTTCTTTAAATGCTCTATTTGATCCAGGCATACCAAACATAGATCTTAAGAATGATATAATTCCATCTTTTGGACTTATTGCATTTAAAACAACTGAAAAGACTTTTTCTTCATCTTTGTTTAAGTCTGGATTAAATTTAACTACTTTGTAATCAATATAATGTGTTGTTATTGTTTGATTTAATTCACGAGCCAGAATATTAAAATCAATATTTGATAGAATATTAATTAAGCCTTGTTTTACATTAGAATAAGGATTTGTTGGATCGCTATTCATTTTTTTAATAATAGGTTTTAAACCACTAATTAATGAAGGATTCATTTTAATTAATAAATTGTAAAGTAAACTATCACTATTATCTAAATTAATTGAAGCAGCTATATCTAAATTATCAACTAATAATTTAAGACCTTCTTTTAATGTTTTTTGGTCTAATGAATTGAATAATCATTTGATTAATAAACCTGATGAAAGTTTTTGAGTGTATGTTTTACCATTATGAACTACTTTTTTATCACTTTCATTTTTATATCATTCATGCGAGTATTCACTAAATTTTTTAAAATCTATTGAATCAATTATTTTTCAAATTGCATTAATAAATATTTTTGGATCATTTGAAACATTAACTAATGATTCTGCACTTAAGTATTGGTTTAAATCAATAGAAGCTAAATCTTGTAGTAATTTGTATAAGTTATTTACTTCAGCTGCTAAATAAGCTTTTTGTTCAACTAAAGTTGATTTAGAAGCTATTTTAGCTTTTGCTTGTTCTAGAATGCTAAAGAATAGTGATTTGAGAACATCACCACCTTCATAATGACTTAACTCATAAACAAAGTCAAACATTAATTTAGGTAATATTTTCTTGTTCATTTTGCCAGTAGCAAACACTGAAGCAAAGTTGTTATCATTTAACACTTTTTTCATAACTTTTGTTAATTGATAAACTTGCTCAGGTGTAATAAATTGTTGTTTAACTAAATCAAAATTTAATAAATATTTTTCAATTGCATTAGCTAAATAATCAATTTTTCCATGATTTAAAACATCATAAATTAATTGAGATTTTGGACTTAAAAAAAGCATTGGGATATATGAAACATTTTGGAAAGTAGAGTCTTGTTTTACTCAACCATTACCACTTGTTTTAATAAATTTGGTTGCAATAGTTAATTGATATTTTTCCATTCATTTTGACAAAAGACCCTTATCCATACCATTTTGTGGCATTTTTTCTGTATAAACAACTTCTCATGTCTCAGGGCTTACATATTTATTGTTTACATCTTTTTGTCTTCTAACGATTTTGTATTTGCTTCCAAAAAATGCAACACCAAGATTAGTTAAATTATAATCTGGAGTTTTCGAACCATCTTTATTTTGATCGTGTGGAATAAATTGAGTTAAAGGAACAATTTTTGCATTTTTTGTTTCTTTTAATTCACCTGTTTTTTGGTTGTAGTCAATTAATTTTCCAAAATGATATGCAGGTCTAATATACTCAGGATTAGGATATAAGTTACGTCCAATTGATTGTAAAACTAAAGAAGAAACATAAGGTGTAAGTTGATGAGTTTTATAAATGTTGTTCTGGTTTTCGGTTGCTTTATTTAAAATTGAAGGATCATAATATTCATCATATAATTTACCAACATTTAATTTAACACCATCAACTACATAATTCTCATCACCTGTATTAATAAAGTGGAAAACATTTTGTTTTCCTGTTTCCTCATTAATTCCATCAACAGTTAAAGTTTTACGAAGTCCGATATTATCTTGTTTAACTTTTGCTACAATTAAATCAATGATTTTTCTTTTTGTTGATTCATAAGCTTCATTTTTAATAATTGCAAATCTTTCAGATTTAATGTTTTCATTGTTAATTTCATCTAAAAATCTAACTTTATCTTTTAAGAATTGTGATTCTTTTTCAATTTTATTAATTGTTCTAGGAGTGTCTTTTCCAATTAAATATTTTTGACCATCTGGTCTAATTCTTGAATCTTTTGTATATAACTCTAATTGAGTTGTTTCAAAATAATTAAAACTTGAGCCTAATGGAATAACCGATTCTTTTGTAATTTTGTTACTTAAATCTAATGTTTTGTCACTTTTGTATGGGGTAACTATATTCTTTTTACTTTTTCTTCAATATGAAAAAGTTGTTAATTTATCTAACATTTTTTTGTTTTCTGGATTTGTTTTTGAAGACTCAATTAAGTCTTCCATAAATGTTTTGAAAGCACCTAATCATTTATCTTTTGATTTATCTTTATCAACAAAACTTGTGTAGTAGTTTCATCTTAAAAAACGAACTATGTTTTCTTCTTTAGCAATTCATTCAATTGGTAAAGTAAATTTATTATTTTTGTTTAAAGATAATTTCTTTTGAGCTTTTATTGCTTTAGGGTTGGCTGCAAAAACATCTTCAAGATTTCAGTCAGGTTTTAAATTTAAGTTTTCATTTATTTCAGCTCAACTTGGATTATATTTAATAAATTTATCTAAATTGGTTGTTTTATCTTTTTGTTTAAAAAGTAAGCCATTTGGAGTACTGCCATCAAATGAAGTAGGTTCAAAATTAAATAATGCAGCAAGTTGTTTAGGTTCTATTTCAATACCTTTTTCTTGGTTAATTCAACTTTTACCTTTTGAATAGTCTGCTGTTATTTCCTTTGTGTCAGTATTAATAAATAATGAACTTAATTGAGTTGCATAAATTTCATTATTGTTGATAGTTAAATTCATTAAATTACCTATTTTATAGGTTTTATCGAAATGAACAATGTTAGTTCTTTCAAGTTTCTTTTCAAGAAAAACTTGTTCATAAGTATCGCCTTTCTTTTCATAAATATTCAAGAAACGGTTTTTTTGTTTTAGTTCAAAAGTTAAATTATTTATGTCTTTTAAATCAAAAACAATGTCTGGGTTTTTATTATTTTCAATGTTTTGTTCAGCTTTATAAATTGAGTAAAGATTTAATAAGTCATCTTTTTTAAAGTAATAACTATTTACCGAAGGGTCAATATTACCTATTTTATTAAATGGCAAATATTCTCTTTTATCATTGCCAAAAAATAAAACATTTCTTGTAAAGTCTTCATATTCTTTTTTAGTTGAATCGGGCAATGAAGAAGCTTTTACACTTTGATAATCAATTTGATATTCAATTGGTGTATAGTCTTTATTTGTCCCTTTGAAATCATCTGATTTTTCACCATTAATGTAGTAACCTTGATTATATGCACTACCTTGTGTTGGTAAATTTAAGTCAACATTGATATCTTGCAATTTTGATACATGTTTATAATCATTAAAACCATTAACCATTGAAGAGCTAACGTTTGATAGCAAGGTAAAAATTGCTGAAGTTAAAAAAACTAAAATGCTTAGTCCAGCAACTATAACTTTATTTTTAGAGAGAGATTTAAAAACTTCTTTGAATAATCTTCACATACTTTCTCCTAATAATTAATATGTATTATTATAATAAAAAATATTTAATATTAAAGATTTAATTTTTTATAGAAAATTACAAAAATTAATTTTTGTAAAAATTATTAATCAATAGATTATGTTTCTATTTTTAGAATTATCTTTTTTATAGGATTTTTAGTCAAAATTTCAAATAAAAACTTGCTCTTTTAAAGCAAGTAATTATGATTATTTTTTAGAATTATCTTTTTTTGTTTCAGCTTTTGAATCTTCTAATTTTTTGTCATCTTTGTGTTCATTGATAAGCTCATCTAAAGTATAAACTTTGGTTTTGATTTTGTCAGCTTCTCTTTTTGGAGGAAGTTTCATATTCTTTTCAATGTATTCAATTTCTTCAGCAACAATTGTTTCATTTTCAAGTAAAGCTGTCTTGATTAATTCAAGAAGTTGTCTGTTTTCTTTAATGATTTTAGCGGCATTCTTTTCTGCTTCTGTCATTATTCTTCTAACTTCAAGATCAATTTCGTGGGCTACTTGAGAACTAAATGAAGCACTTTTTAGATAATCACGGCCTAAGAATGGAGAGCTTTCATCTTTTTCATATTGAATAGGGCCAAGTTGTGACATACCAAATTCAGTTACCATTCTACGAGCAATTTTGGTTGCTTTAGAAATATCATCACTAGCGCCTGTAGAAACATTGTCTTTTCCATAAATTAATTCTTCAGATGCACGACCACCCATAAATCCAGTAATCATTGCAATCAATTCTTGTTTTGTTGCATTATATTTTTCTTCTTCGGGTGTCATTAAGTTGTAACCACCGGCATTTCCACGAGGAATAATGGTAATTTTTTGAACTTTGTTTCCACCTTTAACTTTTAATCCAACAACAGCATGACCGGCTTCGTGGTAAGCAACCATTGTAAGTTCTTCTTTAGTAATAACTCTATTTTTCTTAGCAGGTCCAGCCATAACTCTATCGATACCTTCATCAATATCTTCACGTCTAATAACGTCTGAGTTAGCTCTAACTGCTAATAATGAGGCTTCATTAATAACATTTTCAAGTTGAGCGCCAGAAAATCCTGGAGTTCTTTTTGCAACTTGATCAAGTTTAATTTCAGGTGATAATCTTTTACCTTTAGCATGCAATTCTAAAATTTCTTCACGTTCTTTAACATCAGGTAATCCAACTGTAATTGTTCTGTCAAAACGACCTGGACGAGTTAAAGCAGGGTCTAAAACGTCTGTACGGTTAGTTGCTGCAAAAATTAATATTCCATTATTTTGAGTCATACCATCCATTTCAACAAGAAGTTCGTTAAGTGTTTGTTCACGTTCATCATGACCTCCGCCTAAACCAGCTCCACGGGTACGACCAATAGCATCTAATTCATCAATAAAGATAATAGCTGGAGCATTTTTACGAGCTTCTGCAAAAACAGTTCTAACTCTTTTAGCACCTAAACCAACATACATTTCAACAAAGTTTGAAGCTGAAATAAAGTAGAAAGGTACATTTGCTTCACCTGCTGTAGCTTTAGCTAACAATGTTTTACCTGTCCCTGTAGGGCCTCCAAGTAAAATACCATGTGGCATTCTAGCACCAGCAAGTGCATATTTTTTAGGATTTTTAAGATAATCAACAATTTCTTCAATTTCTTCAATTGCTTCTTTGTTACCTGCAACATCTTTAAATGTTTTATCTGATTTGACTTTTCTTGCAGGATTCTTTACATCTCCAATTGCACCCATCATACTCATATTTTTTTTCATCAAGTATCTGTAAAGGAAAAATAATAAAAGAATAGTAATAAATGTTGGAATTAATGAAGTAAGTAAACTTCTTAATAATGAAGGTTGTCCATCGGGAATAGGTTTAGCTAAGTAAGATCCACCTTTTGTTAGGACAAAAGTTTTTAAGAATTCAAAAAAAGTTAATCCTTTAAAACCAGCTTTATTACCTAAGACATTGGCTAATCCTTCTCCAATAATTTCATTAGTTGAAGATCATGATGCTGCATTAGATCTTCCAATTTGAGCAATTCTTTCAACTAATATGCGGTTACCTTTTAAGTCTAAACCAGCATATACATATTTTGCAGTTCCATAATACGAGTCAAACTCTATACTTGCAAAATAAACATCATCTGATTGACTAGCTGAAGCAGCTTTTAAATCATTCATAAATTGATTTAAGTCAACAATTTGAGGCTTAGGTCCAAATCTTTTAAACAAATAATATGAAACGATTGATAAAGCTAAGAATGACAATACTGTAATTAAAATAATTGACAGTATGCTTGGTTTTTTTTGCATAAATGCTCCTTTCTTAATTTGGTTAATAAATAAAACAAATTATACATTAATATTTATATTAAATAGTAATTTTACTTTTTAGTAATAATTTTCCTTGCGACTTTTGCAAATAATATTGACTGTTTAAAATAAAAATAGGATTATTTGTATTATCTGCAATTACATAATTAACAAGATTTTTAATCTTGCCTGAAGTTAAATTGATTTTATCTTCGCAATGAGCAAAAATAAATTTTCTAATTAATTGCTCTTGAATTTCATAATCAAGATCTTTATAATAATTAACCTTGAATTCTATTTTTTTTCAGCTTGCAAACGCTTTATTAAATTCTTTTTCTAATCATTCTAATTCTTTGTTTTTTTCATTAACTTCATCAATAATTTTTTGTTTTTCTTCGTTAGTTAATTTCTTTAGTTTTAATCTAAATTTATTGCGTGTATAAATAGGCAATTCATTGGTGTAATCAATTGCATAATCTAATTTTAGTTTTTTACATTTTTCTAAAATTTCATCTTTAAAATACTTATCAATAAAAGGTCTTTCAATTAAACAGCCTTTATAAATATTTGTTTTTTTAATGCCTCAAAATGAAGGTTTTCTTTTCGCTACTTTTTGCATAAAAAATGACTCTAAAAAATCATCTTTGTGGTGTGCTAACAAAATTTTAGAAGCATTCTTTTCATGATAATTTTGAATAAAGATTTCCATTCTTTTGTCTCTGGCCCATTCTTCAAAATTGCCTGTTTTATAGTCTTTTTGGTCAAAATTGTATATTTGATAATCTATAAAATTTTGTTTGCAATAATTTTCTACAATTTCCACATCTTTATGTGAATCCATCCTTTGATTATAATTAATGCACACTACAAAAATGGGCTCTTTTTTATAAGCATATTCATGTAAAAGTAGCATTGAATCTGGGCCGCCACTAATTCCTAAAATATACATATTATTTACCTTTACGAAAAACATTAAAGTTATTCATTACTATCTTAATATCATTGTAAACAAAAGATATTGCAGCTTGTGCAGCAACATTTAGAACTTGTTCGACTACTAACATTTGATCACTTGTAAAATTACTTAAAACATAATCTTTTAATTCCATTTTAGGATCTCTTCCGATTCCTATTCTAAGCCTCTTAAATTCATTGCTTCTAAGAGCATTAATTATGCTTTGAACACCATTATGTCCAGCACTTGAACCGCCTACTTTAATTGCTGCTTGACCAATTTCATAATCCTTTTCATCATGAATAATCAAAATATCTGAAGGATCTATTTTATAAAATGAAGCTATCGCTTGAACAAATTCACCAGAATTATTCATATAAGTAGTTGGCTTAGCTAAAATAAAATCTTCAGTTTTTGCATAAAAGCCATTAAATTTTTCTTTTGTAAGAGTTAAATTAAAGTTCGCACAAATTTTGTCGACAGTTAAAAAACCAATATTGTGTTTTGTAAAACGATATTGGTTTCCTGGATTACCTAAACCTACGATTAATTTCATATGTTAATTATAAATTATTTTTATTTTTAAATTCAAAATTCATATTTGTTAAAACATTAATTTCTTTTAGTTTAGGTACTGTTCTTTAAATTCATCTACATCATTCATAATAATGATTTTCTTTTTAGCTCTTGAAAGAGCAGTATATAAGTGATTTTTAGTAATATATTTGGCTATGTTTTTGTCAAAGTAAATGATAACTACATCAGATTCAGAACCTTGAACTTTATAATAATTCAAACAATAAGCTGATAAAAAATCTTCACTTTTTTTATTGTAAATGATCTTTTTTTCTAAGTCATCTTTGTAGAATCTTAATTCAATTAGATCTCTTTTATAATTTTCTAAAGCTTGACCTAATTCACTATTATAAATGTTTAAATGATACTTATTTACATTTTGAATAAAAGCATAAACAAAGGAACTAAATAAATTTTACATTACACCTTGGAAATAAAAATCAATATTATTTAATTTGTGAATAACATCTCTAACTTGTGAATTTAAAGGAAACAAAGCAACTACTTTTTCAACTCAGTTGGTTTTCCTTCTGGATAATTGCACCCATAAAATTCTTTTAGAAGTGCATAAATAACAAAAAGTTCATCTTTATAATTTGGGAAACATTCTTTAATATTTTTTGATGAATCGATAAACTTCGAGTCAATATAATTATAATAATATTTGAGAGCTTCGTATTTTCTAGCCATATCATTTTGATTAAATGCAATTACATTTTCACCATCAAAGTGAAATGGTTTGTTTTCTAAAATACTTTCATAGAATTCTTCAATATTTTTGTTCGCACGGAATGAGCCTTTAAGTTTTAATACTTGTGAATCTTCAGAATAATAATTAATGATCTTTTTTTTATCATCAAAATTGTAATTAATTGAACACAATGTGCCAATTTTAAGATCTTCTAATTCAAATTTGATGTGAAATATAACCTTTTATTTTGTTATAAACCATTTTGTTGATATCAAAAGATTCAAAAACAGCTAAAACACCATTCACGTTGTGATTTTTTAATTTGTTTAAAACCATTCGTTCATTTGTATTAGTTATAAACATTATGAAAATTATAATAAATTATTTATTAATTTTGTTCCTTAATTAAATCAAATAAATATGTTTTTATTTTACTATAATCTTCAGTTTTTGTTTTTTTAGCGTATAAAGCTAAGTCGCCTAAAATAAATAATTTTTCTTTAGCTCTTGAAACTGCTGTATATAAAAGTTTTTTATTAAGCATTCTTGAATAATCTTTAAAAACCGGAATTACAACATGTGGCGCTTCTGAACCTTGAAATTTATGAACAGTTAAAGCATATGCTAAAGATAAATTAATTGCTATTTCTGATTTTACATAATTAACTGTTTTAAATTTATTTCCACCTTGTGGATATTCAACACTAACAATTTTGTCATCTATTATTTCTTTTATTATTCCGATCTCACCATTAAAAACATTTTTTTCATAATCATTTATTAATTGGATAACATTGTCAAATAAATAGAATTTTGTACCTTCTCAAGATTGGATATAGTTGTCTTTACTTTGTTTAAATTTAGGCAACTTATATGTGTAATTATTTAGTTTTTCTTTTTGCAATCATTTATTAACTGTTTTGGCTCCAACTTGACCATCATGAAGTGGAATCAAGGTTACCATTTTTTCAGAATTGTATTCTGGATTATAAGTTTGATATAAGTATGATAATTCGTTTAATAATGCAACTGAGTTTATTTCTTTCATTTGAATTGTGTTTGTACTTCCTATTTCAACTTCTTTAACTTTTGAATCAATAATACTTAAAAAGTGTTCGCAAATTTCATCACTTTCAGCTCTTTGATTTTCAATTAAAAGAGTTTTAGGAATAAAATCTCAATTACATAAGTCGTGCATTAAATTGCCAGGTCCAATACATGGAAGCTGATCTTTATCTCCAACAATAATGAGCTTTTCAAGCTTGGATAAATGACTTAAAAGTTGATAAAAAATCTTTAAATTAACCATTGAAAACTCGTCAATAATTACAACTTTCGTTTTGTCACAATCATTGTTATAAACAACGTCTTCATCTTCTTTATCAATTTTTAAATAGTTGTGAATCGTTTTTGCAGGCAAATTATTACTCTTATTTTTAATATTAATTGCAGCTCTACCAGTGGGGGTCAAAATTTCTATTTCACCTTTTTTATAGACTTTTTGGGCAATTAATGACTCATAAATATTACTAATTAAATATGTTTTGCCAGTGCCAGGGAATCCTGAAATTATGCTTATCGGTTCATTTAGTGCAATGTTTAAAGCATCAAGTTGTTTAGCAGTTAATGTATCATCTTCTATTTGCGGAATAGTTTTATTTATCTTATTTTTTATAGTTATTAATTTTTCAAAAATAAAGTCTTCTTGTTCTTTAATAAAACTAAGTGCTATAGTTGGAACTTCTTTTTCTGCATAATATAATTTTTGCATTTCAATTAGTTTGTTTATAGAATTAATAAATATATTATCAATAGCAAGCTTTGAAATTGATAACTTATTATTTAGTATTAACTCATTTGTAAATTCAAGTCAATTTTTATGTAAATCAGACAAATTGTAATATGTATCATTAACTTGAATTCCAAAATCATAAATAGATTTATAAACTATTGCATAATATCTAGTTTCGCAATCTTTTGAATATCCTATTAAGCTTGCAAAATTGTCTACTGAGTCAAAGCTTAATCCTAATTCAATATACAAAATAAGATAAGGATCTTCATGATTTTCTTTGTTTTTATCAAAAAAATCTAAATATGATTCGCCATCTGCAAGAGGTAATTTTTGCTTCAATTTAATATAAAAATTGTAAATATTATTGGTTATAAAAAATCTTTCATCTTTACCCATGTTTAGAACTTCTTCAATAAAAGCTTCTATTTTTTTAAGCAAAGGGGGTTTAATAAATTCGTAAAATATTTTTCTTTCTTCTTTAGGTGTATCTTTAATAACATCTAAATAATTTTCACCTAAATTTTCTTTTAAAATATTATGATCTTCTTCTTTAAAACCTCTAATATGATTCTTCAACATAACTTTTAAATCTTCTTTTGTTGCTTCAATCATATTGTAGCCTTCAAGTATGTAATTATTATATTTATTAGGCCCACTTAATTCCATTTCATATAAATAACCAACTTGTAAAAAGTCATCGGAACTAATTCGAGCATAGACAACAAATAATTGGTTTTCTTGAGATTTAAATTCAAAAAGAATTCACTTTTGAGCCAATCCTTTTCTTATTTTTTTTATTGTTCCTTTTACTTTTATTGTTGTTTTTGCTTGCATATTATTAAATTATATTGAATAATATAAAAATAAATATGCTTAATTTTAAAAAATAAAATAATAAAAACAAAATATAAAATCCACTTTCTAGTTTCATATGCTTGGTAGTTTGTACTTATTATTTTAAACGATGAATTCACTATAAAGATAATTCTTTAGATCTAAATTATAAGTTTTATTCTTATTTGATCACAAATTACCATCATTAACTACTAAATTTTTATATAGTTTTTAGAAATTGAATCTTCTTTTCTTTTGAAACTAAATTTAAATCTAATTGCTGAATTATAGTGGTGTAACATATTTGTAGCTATAAAAGTTGATAAAATTTGAAACAC", "length": 1118751, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000186005.1|CP002458.1", "orig_description": "HMT-607 Mycoplasmopsis fermentans M64" } ], "run": { "start": "2026-01-27 08:29:52", "end": "2026-01-27 08:37:59", "duration": "8.12 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }